LOCUS       DAYAPA010000001        68693 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.001, whole genome shotgun sequence.
ACCESSION   DAYAPA010000001 DAYAPA010000000
VERSION     DAYAPA010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 68693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 68693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68693
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.001"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(365..1087)
                     /locus_tag="ACGI59_000001"
     CDS             complement(365..1087)
                     /locus_tag="ACGI59_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFG9737817.1"
                     /translation="MITIKNLSKSFGDLNVLKNIDLEIAKGEIMVIVGPSGSGKSTLL
                     RCMNLLEIPTGGEIIFEGKNLVDKKTNIDEVRQNIGMVFQNFNLFPHKTILDNITLAP
                     IKLKKMTKEEAEKKAEILLSRVGLLDKKDSYPSQLSGGQKQRIAIARALAMEPDMMLF
                     DEPTSALDPEMVNEVLDVIKELAKEGMTMAIVTHEMGFAKEVADRVIFIDGGSILEDN
                     TPEEVFGNPKHERTKAFLAKVL"
     gene            complement(1146..1823)
                     /locus_tag="ACGI59_000002"
     CDS             complement(1146..1823)
                     /locus_tag="ACGI59_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFG9737818.1"
                     /translation="MSLDFSFLSRFGTSFLEGTGVTVSISLVALCFGFIIGIIICMAK
                     ISKSKVLRAISSIYIEVLRGTPLLVQIYIVWFGLPQLGIRFPMLFGIPSEFIASAFAL
                     SVNSGAYVAEILRSGIQSVDNGQMEASRSLGLNYWSTMRYIIIPQAIKNILPSLANEF
                     ITLVKESSIISVIGVVEIMRTADIVKNAAFRALEPLIVAAAIYFVITFTLSRLVGLLE
                     KKLSVSN"
     gene            complement(1851..2666)
                     /locus_tag="ACGI59_000003"
     CDS             complement(1851..2666)
                     /locus_tag="ACGI59_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFG9737819.1"
                     /translation="MKVFKKLLSLGLVLGLTLSLVGCSGGGEKTKLEQIKDNGKLVVG
                     TSAEFPPFEFHKVVDGKDSIKGFDIMLAEEFAKELGVKVEIKDMSFDGLIGALNADQV
                     DIVLAGMSPTPEREKSVDFSELYYLSRNAVIVKDADIDKVKTEDDLKKLRVGVQAGSI
                     QEEYVVNTLKMTTTKSLKAIPDLITELKNGNIDAVVTNEAVSLINVKKYDGIKMANTE
                     VGKDVTEGMAAAIKKSDNNKDFIELLNKKIKELQDGKKIEEFLNEASTEAASN"
     misc_binding    complement(2892..3102)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(3694..5760)
                     /locus_tag="ACGI59_000004"
     CDS             complement(3694..5760)
                     /locus_tag="ACGI59_000004"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFG9737820.1"
                     /translation="MVELIITTNIDIRKLNENQLEAVEHIDGPCMILAGPGSGKTRVI
                     TYRIANMVVNKNIAPTRILAISFTKASSIEMKNRALNLSDDIRLNKVTYGTFHSVFFK
                     ILRYFERYNLDSIFDEKSKRMTIKAILKSLNIENADDDENIGQVINEISYVKNELMDK
                     NEFNSEVLTKDEFLKVYNLYEEQKSKVNKIDFDDMLIRTYYLLLNNKSALEMVRNVYK
                     YILIDEFQDINKVQFEVLKLICSPLNNIFAVGDEDQSIYGFRGARPDFLLEFEQYFNN
                     TKKIILDINYRSKSEIVHTANRLIDKNKNRYEKIIKCSQGDGGSVTYISPHDSEEEAL
                     YIAREIIDEIQKDYVEYSDFAVIYRTNIQSRALVDVFMDMRIPFVVKDSVITIYDHWA
                     SQDILAYLRIGINPKSNKDWLRIINKPFRYISKDSINMVKDEKDFITALINKCNLHPK
                     QVKTINDLEIDLSYLNTLNPKNAISYIRTSLDYDRYVLDYCSNRKIKTNGLVEILNEL
                     ESSATNFNTVTEFLEHIDRVKTELTENNKNKQTEGVIFTTMHSAKGLEFRNVYIIGVN
                     EGTIPHEKSYDICKEEKKEEQLEEERRLMYVAITRAEEKLCISSTLNKYGKKVDKSLF
                     INDIKSPTKKEIDSIGVGDKVYHKKFHEGEIIKKDGIMFTIKFKDRERILDLKTCLLK
                     NIIYTI"
     gene            complement(5837..8866)
                     /locus_tag="ACGI59_000005"
     CDS             complement(5837..8866)
                     /locus_tag="ACGI59_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFG9737821.1"
                     /translation="MENNLRKNNSIMKKMIFPLIIVMLIQTSLFCATILWGGTIKKLN
                     DNSFDILNERVINRKNYIQNEMLQRWSNTSETEATINSSVKKVLNEKNALIEDVGNNE
                     EINKDIIKSISNDLIYLLRKNSVTGAFIILSDKNINDKSIDSKGEISRTGLYIRDLDP
                     KFNPNDNSDLLIERGSSDIARSFGISMDSYWEPKFSFKSENEQDGDDFFYKPFRAAID
                     NPNVSSSNLGYWGSKFLLTNAGDRLMTYTVPLIYEDGTVYGVLGIDLSVNYLRELLPY
                     SEINGNKKGNYILGVDRNNDMAFENVISNGYSLKEKFWDGLHSNLEQKESYKNIYKIK
                     NKDNECGNGNIYASVQYLDLYNSNTPFENERWALLGIIEEKSLLSFSKRVEKLVIISI
                     AISLLIGLIGIYIASRLFTKPIVLLANKVRNSNPSKPVNLDKINISEIDELSSAIELL
                     SANVADSSSKLSQIIEMVDMPIGAFELYKNAENVFCTKEFFSVFTTEIKFEEYGYMEK
                     NKFLKKLRGLDKYLQESYETENTYIYKFESKDKKLKWIRMNIFEDELKVLGVVVDVTK
                     EFIEKRKIEYERDFDILTNLLNRRAFHSQIQEKFKNKDELKIAAFIMWDLDNLKYIND
                     TYGHDAGDEYIRCAADILKKFISWNGIVSRLSGDEFYVFIYGYESKDSIRDIINSVKK
                     QMNNTLLKLHDGTEFKIRASAGISWYPDDSTNYDELMRYADFAMYQIKNTIKGEVSEF
                     DINIYNKDSFLLSNREELNKLIDGQLVEYAFQPIVDCKGKVFAYEALMRPKIKTFSSP
                     IDVIRLARSQSKLYQIERITWFKALKSFKKYKEKFGDCKLFLNSIPNNNLSKVDFEEI
                     EDLYGEYLNRVVLEITENDKINEDFIEKKKAYIDHWNIEIALDDFGTGYNGDAVLLYI
                     MPDYVKIDMSIVRGIDKDENRQKILKNLISYSNERNIKVIAEGVETKGEMETLINLGV
                     DYMQGYYISKPSFIPEDISDKIKKELKICHENRNDVV"
     gene            complement(8919..10379)
                     /locus_tag="ACGI59_000006"
     CDS             complement(8919..10379)
                     /locus_tag="ACGI59_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFG9737822.1"
                     /translation="MNRCKFAKLIITSMIVLLLTGCSLKKEDNYKLDPENPTSIQIWH
                     YYNGQQKMAFDKLVKEFNDTVGAEKGIIVEAFSQGNVVDLSEKVLDSANKKVGSEDVP
                     NIFAAYSDTAYEMDKLGLLVDMDKYISKEELNEYIDEYVEEGRINSKDEFKLFPIAKS
                     TEILMLNKTDWDKFAKATNSNINDIKTIEGLVETSKKYYEWTDSLTKEPNDGKAFFGR
                     DALANYIIIGSKQLGVEIFSVKDGKVTLNIDEKVMKKLWDNFYVPYINGYFASYGKFR
                     SDDAKTGDILALVGSSSGAAYFPDRVMLNDNESYNIETSVFQAPRFKGEGKYAVQQGA
                     GMAVTKSTKEEEYASVEFLKWFTDKEPNIKFSIASGYLPVKKKTNDLDVIKEVMKKDN
                     LSLSDKVEGSIASSIEQINSCNLYTNKAFDGGTKARQILEYSMLDKAKEDSNKINELV
                     KSGTSKKDAVAQFNNDKNFKTWFESFKAELENAIKK"
     gene            complement(10471..10845)
                     /locus_tag="ACGI59_000007"
     CDS             complement(10471..10845)
                     /locus_tag="ACGI59_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9737823.1"
                     /translation="MGNKNKNTKYKDKTRERYNKKVENLNKEKLNKINKQSKKVDKID
                     DIINKSSIVLCVLLVVFSLLYLFKLVQLNIIMILLVITVAIQQGLVSFGAFRNKNYVK
                     ALFSIFVIVCFLIAFKYVVNPV"
     gene            complement(11021..11761)
                     /locus_tag="ACGI59_000008"
     CDS             complement(11021..11761)
                     /locus_tag="ACGI59_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HFG9737824.1"
                     /translation="MLYDEDEKEENNERWLLTYSDLITLLMIFFVIMYSMSNVDAEKY
                     KQLSQSLNSAFGGSSGVIEGGNSKIEPVVEPGSNDLDTLQNAKFKKIGEEIQKYLNEN
                     GMANSVSLRVQDRGLVISLKDTILFDTGKAIVKDNSRDKIIQIGKMLNEMNSYMRVEG
                     HTDNMSIKNSEFKSNWDLSVMRATNVVQLLIDNAGIAPDKLSAVGYGEFRPIAENSSE
                     EGRSKNRRVDIVLVDSKYDNVENVSKDK"
     gene            complement(11782..12570)
                     /locus_tag="ACGI59_000009"
     CDS             complement(11782..12570)
                     /locus_tag="ACGI59_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein"
                     /protein_id="HFG9737825.1"
                     /translation="MTTLIFFIVGIASLVVAFVIDGGHVGALFVFSSALVVFGGTIGA
                     VGASTPFPVFKKSIKIMLLAFKNKKPNNIENIIYFKEIATKARKEGLLSIDKEISEND
                     ELDPFIRKGLELMVDGVDAVTIRNILESQIQITSKRHKEGIAIFDSAGGYAPTLGIIG
                     TVMGLVHILGNLSSNPDALGPQISVAFLATLYGVGSANLIWLPIASKLKAINQQEVSE
                     RLLVIEAILSILEGDNSNLMTEKLKIFLNSKELIELEEIGGRAE"
     gene            complement(12658..12748)
                     /locus_tag="ACGI59_000010"
     tRNA            complement(12658..12748)
                     /locus_tag="ACGI59_000010"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12712..12714),aa:Ser,seq:gct)
     gene            13007..13771
                     /locus_tag="ACGI59_000011"
     CDS             13007..13771
                     /locus_tag="ACGI59_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFG9737826.1"
                     /translation="MLPQERYEKILSILKSDNIIKINDIVQMFDISIETARRDLAHME
                     SIGLLKRVYGGAIPVIASSVELNYVKRKKINSNDKKQIAIKCSEFINTNDTIFMDTGT
                     TVLEVSKNLKDKKNLTVITNSILVANELMNTNIKVYVLGGYLRETEGSFSGPLTLSAI
                     EQFNVDKAILGAGGITLENGISDYHLEEALVRKKMIERSRKSIVVVEPPKFGINSFAS
                     VAPIEHIDTIITSASIDHNIIIEFSQKGIPLVIADN"
     gene            complement(14052..15179)
                     /locus_tag="ACGI59_000012"
     CDS             complement(14052..15179)
                     /locus_tag="ACGI59_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HFG9737827.1"
                     /translation="MPFDKIILYIMAVGVLLGALDRIIGNKFGLGERFEEGFNSMGPL
                     ALGMVGIVCLAPVISDVLGPIIIPIFNLCGADPSMFATILANDMGGYPLAMELAHNKE
                     AGMLAGLVVSSMLGCTLVFSIPVGLGLIEYEDRPFFAKGLLIGLITIPFGGIIGGLIA
                     GFNLSMVIINMIPVIILAVILAVILAVGLKFNSTLMINGSLIFGKFISILITIGLGAA
                     AFQEITGIVLIKGMTPIREGIQVIGSIAIVLLGTFPILHLLVKVLNKPLTIVGGKLGM
                     DATSAAGLVFTLANSIPVYKMMKDMSPKGKVVNTAWLVCATAALGDHLGFTAGVVPEM
                     ITPVVIGKLVGGVLAIALAILMTKDLTLEEEQSIKMKKDAV"
     gene            complement(15220..16746)
                     /gene="glpK"
                     /locus_tag="ACGI59_000013"
     CDS             complement(15220..16746)
                     /gene="glpK"
                     /locus_tag="ACGI59_000013"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFG9737828.1"
                     /translation="MKYVLAIDQGTTGTRAVLINEKSEFVFSDYMEHTQIYPQSGYVE
                     HDPIEIWNNTKTVSNNVLQQAFENGINESDIKGIGIDNQGETVMLWDKNTGKPLYNAI
                     VWQCRRTYDYVENLKNIPGLENKIIDKTGLIIDPYFSGTKIKWVIDNVKGVKEKIKNG
                     EVLAGTLDSWLIWKMTGGKSFLTDVSTAARTMLFNIRDMDWDRELLDIIGVPRSILAD
                     IMPTSGDFGTTDKEAFCGVSIKITASMVDQPAALFGNGCFEPGMVKNTYGTGCFLYMN
                     IGEELNIAGNGILTTVAWKIGDKVTYAYDGGVYIAGAAIQWLRDGIGVIKNYSETDDM
                     ANSISSTGGVYFVPAFAGIAAPYWDQYARGTMVGITGSTKKEHIVRATLESVALQVKD
                     VVDSMNLASGKKIKMLRVDGGITKNNFTMQFQSDILGIPIEIAENPETTVLGVGYMAG
                     LSCGVWNSIDEIRSEFTISKKYIPNMTTQKREEILLGWNQAVKRAMNWENDTKKVCAN
                     "
     gene            17039..18136
                     /locus_tag="ACGI59_000014"
     CDS             17039..18136
                     /locus_tag="ACGI59_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HFG9737829.1"
                     /translation="MKKTYLFSPGPVMVTDKVRNSLLHYDICHRGNEFMDLFKDTQQK
                     INKLYNATSNYYSLIVSGSGTSVNECVLSSIFDKEDAVLLVSNGVFGERLEEILSTYN
                     VKTYKPNFEWAEYPDLDILEKYIIENSDIKVIAMVFHETSSGMINPVPELGKLAKKYN
                     KIFFVDAISASAGEYIDVEEFNIDIITGVGGKALGAFPGSAYLCAKESILQNIKENQC
                     KNVYLNLYKHYKVAKSSSQTPNTPNVTLIFALNEALTEFLEDDSKIERYKECSAILRY
                     GMEELGLTFLLPDKYMSNTVTSVFLPKEINVNEFILGLERESGYVVYPGKGKFLDANI
                     FQVANMGEIYPEDCYKFLDILKSKLEIKKVI"
     gene            complement(18627..19139)
                     /locus_tag="ACGI59_000015"
     CDS             complement(18627..19139)
                     /locus_tag="ACGI59_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9737830.1"
                     /translation="MKVKKFKSGIVMALTVMAIGASLTNVSAMELNKSKEFETKYNEM
                     KRDFQEEYGKEDKFNITLDDEFTKEEVNGAKGIIKTTNKMNNEVVVLNYFDEMKSGIE
                     FELKPKAEKNAILRDEFQQGYGKEDKFKVVLDDEFTKEEVNGAKGIIKTTDKETGEVI
                     VYNYFDELEK"
     gene            19692..20573
                     /locus_tag="ACGI59_000016"
     CDS             19692..20573
                     /locus_tag="ACGI59_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4300 family protein"
                     /protein_id="HFG9737831.1"
                     /translation="MKKRLIIMILSVVLVLSSILTIFAYSNIKYNNNKLIYSNMIDKK
                     TQNSVKEILKENKINEKDIDTFIKSVNNYNKLQVKILQNNINISKSGYSSINAKQVPY
                     NLEKLQDNWVKKFPDYMDVNCRITAFRLFKDFINSNKKFTGDSIDLNVDLDTIMNNKD
                     AKFSTKDVEKFINFFSAIPAKDTDDTIKIAEQIKNEWKKRKISFKNNKNISIINGFLR
                     YPETKNVFIGHTGICIKTKNGILFLEKYGVTSPYQVTKFKNKEDVKNYMFNRLKMSEG
                     EIELPDPIIMENDKLMK"
     gene            complement(20886..21665)
                     /gene="hisJ"
                     /locus_tag="ACGI59_000017"
     CDS             complement(20886..21665)
                     /gene="hisJ"
                     /locus_tag="ACGI59_000017"
                     /EC_number="3.1.3.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ"
                     /protein_id="HFG9737832.1"
                     /translation="MKDGHIHSPYCPHGSKDDFEKYVQRAMDVGITEMTFTEHFPYAN
                     GFRDPAPENDSCMSIEDLPKYFNDVKKLKEKYEGKIKINVGSEVDFIEGYEEGIKENL
                     NKYGKELEDSLLSVHIIKIDDDYYCVDYSVEEFQNLIDKLGSIEAVYNKYYETLIKAV
                     NSDLGIHKPKRIGHLNLVRKFNQVFPYDYKGNKILEELIKLIKEKGYELDYNVSGNRK
                     EYCKEPYIDGYLLDLVKKYDIPLVLGSDSHCAEEINKYDII"
     gene            complement(22211..22561)
                     /locus_tag="ACGI59_000018"
     CDS             complement(22211..22561)
                     /locus_tag="ACGI59_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter"
                     /protein_id="HFG9737833.1"
                     /translation="MIFDKEIVRYHHGHHDHDHCHEHTHGDICHEHPHDHAHDHDHEH
                     SHEESSESKDEKTLKILLVHWINHNETHEEGFREWVEKARAIGKEETAKSIEKAIEYM
                     EEANKMLLEAKKHM"
     gene            complement(22577..22993)
                     /locus_tag="ACGI59_000019"
     CDS             complement(22577..22993)
                     /locus_tag="ACGI59_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3842 family protein"
                     /protein_id="HFG9737834.1"
                     /translation="MIIAVIDGMGGGIGAQIVSSLREELPTYVEIYALGTNSIATSSM
                     MKAHANKGATGENAIVVSAKKANIIVAPISVIIPNSMMGEVTCNISEAIADSEALKIL
                     LPIMPENVELVGLEGKPLALLVKDSVNLIKKEFNIK"
     gene            complement(23012..23203)
                     /locus_tag="ACGI59_000020"
     CDS             complement(23012..23203)
                     /locus_tag="ACGI59_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CooT family nickel-binding protein"
                     /protein_id="HFG9737835.1"
                     /translation="MCESSAFICKSNNELEKVMENVVNIDPCDGKIYLTDLLGEQKII
                     DGIIKEIRLMDHKIIIQEN"
     gene            complement(23354..24169)
                     /locus_tag="ACGI59_000021"
     CDS             complement(23354..24169)
                     /locus_tag="ACGI59_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoate A/B protein ligase family
                     protein"
                     /protein_id="HFG9737836.1"
                     /translation="MNQWRVIHNKSYEGAMNMAIDEAIFTAYKKGHNKPTLRFYTWEP
                     ACLSIGYFQKLEDEIDLDKCRCMNIDYTRRITGGRAVLHDNELTYSIIIGEDNPLIDK
                     SINLSYRYISEGLVKGLNLSGIETDNLNRGERISRENLSAACFNAHASYEVTINNKKV
                     IGSAQSRKDGVLLQHGSIILDFDVEKLFKLIKTKTPELKERAMKFTAKKASGIENEIG
                     RKIDIDILQKNIVKGLAEQFNVEFVEGDLTDYEKQLVKELYEKYKNEEYNKKR"
     gene            complement(24216..25154)
                     /locus_tag="ACGI59_000022"
     CDS             complement(24216..25154)
                     /locus_tag="ACGI59_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFG9737837.1"
                     /translation="MIRLSVGTAIELGILNKKSDIPPTTAYIMIGEKCINKCSFCSQS
                     IESSTRKDKLSRVIWPEFSKEEILDALKAYKGKNIKRICIQSMASEEAHNSVLDFINY
                     ISGKIDMPISLSAKLENDEQIKKFFSAGVDKIGIAIDAANKELYEKIKGNNYDEKLKF
                     ITEMSKSYPNKISTHIIVGMGESHEDIYNLYTYLKENDVTISLFAFTPVRGTKMEKIN
                     QPSIESYRRVQLMSYMINKGYPKEYFKFKNGYLNSIKLDNDILKDINKGYPFEIRGCK
                     DCNRPYYNERPGSTIYNYSRPLNQSEIDLAIREINL"
     gene            complement(25151..26023)
                     /locus_tag="ACGI59_000023"
     CDS             complement(25151..26023)
                     /locus_tag="ACGI59_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFG9737838.1"
                     /translation="MNKNLSEKMKIAFDIKLKNFGNKIEFAYPNQTLALSTTSNQCSL
                     KCAHCNGHYLNNMVPIEEYEEKVQSRNITSFLLSGGCSYEGDVPINTHINTIKNLKEQ
                     GYRLNAHLGLMDKDSIVELCKYLDIVSFDLVFDDETIREVYKMKKSKEDYIEVYNTIQ
                     EHTEVAPHICIGLKGGQIKGEYEIIEYLQKNPPNKLTFIVLIPTKGTEYENVEPPELE
                     GVADILCEARINLPDTEINLGCMRPRGVYRKELDQLSIMCGVNRIVLPSRSAKNKAIE
                     MNMTINECKECCVL"
     gene            complement(26048..27976)
                     /gene="acsV"
                     /locus_tag="ACGI59_000024"
     CDS             complement(26048..27976)
                     /gene="acsV"
                     /locus_tag="ACGI59_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="corrinoid activation/regeneration protein AcsV"
                     /protein_id="HFG9737839.1"
                     /translation="MIKVSFTPNNKEVYCNEGDILLEVARNADIFIDAPCNGNVSCGK
                     CKVKLLNGKVDTEKTRHITDDEWEQGYILACCTKVISDIEIEVPSKVSSSMHGMKIEG
                     SNKKEDREIFERAKKIIEEHNLQFKTNIKKKYIEMEEPNLDDNISDVDRLERYVRNNL
                     GYNEIDFRLDILRKMPTVFRKSDFKVTITYVQKQKKLTIINIEQGNKENSLYGVAIDI
                     GTTSVVVCLVDLYSKEVVDKASSGNAQIKYGADVINRIIYSTKKNGLETLHKAIVEET
                     INPLLKTIYERNGIDKEDVVTLVAAGNTTMTSLFLGVYTDFLRQEPYIPPFLKSPKLM
                     GENVGLFVNDSAYVYLAPSVASYVGGDITAGVLSAGIWSSEENVLFIDLGTNGEIVFG
                     NKDYMMSCACSAGPAFEGGGISCGMRASAGAIEKVIIDKDTLEPTLKIIDECAPVGIC
                     GSGIIDLICQMITKGVIDRRGKIYRDLDNKRVRFNEHEIGEYVLAFKEEFDLENDIVV
                     NEVDIDNFIRAKGAIYSGAYTLVDSLGMDFSILDRVYIAGGIGNNLDIENSIIIGLLP
                     DIDREKFTYIGNSSLVGSYLALISKDAKNKLEEIGNQITYVELSVYPSYMDEFISACF
                     LPHTNIEQFPTAKKLLEE"
     gene            complement(28088..28465)
                     /gene="gcvH"
                     /locus_tag="ACGI59_000025"
     CDS             complement(28088..28465)
                     /gene="gcvH"
                     /locus_tag="ACGI59_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HFG9737840.1"
                     /translation="MKLLPELKYSKDHEWVKVIDGDVVYIGITDYAQDQLGEILFVET
                     PEVEDTVTKGVDFGVVESSKVASDLISPVNGEVLEVNEKLEDEPECINEDPYENWILK
                     VKLADVAELDTLLSDKEYEAGLE"
     gene            complement(28552..30678)
                     /gene="acsB"
                     /locus_tag="ACGI59_000026"
     CDS             complement(28552..30678)
                     /gene="acsB"
                     /locus_tag="ACGI59_000026"
                     /EC_number="2.3.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit alpha/beta"
                     /protein_id="HFG9737841.1"
                     /translation="MNLYNIIFTGSEQALGAAQAMLAEAIEKNGKEHKVAFPDTAYSL
                     PCIYAATGQKMNTLGDLEGALEVVKSLINRTHLLEHAFNAGLATALAAEVIEALKYST
                     MDAPYSEPCAGHITDPIIRSLGVPLVTGDIPGVAVVLGECPDAESAAKVIKDYQSKGL
                     LTFLVGKVIDQAIEAGVKMGLELRVIPLGYDVTSVIHVVSVAVRAALIFGGLTPGDLN
                     GLLEYTANRVPAFVNAFGPLSELVVSAGAGAIALGFPVITDQTVLEVPMNLLTQKDYD
                     KIVATSLEARGIKIKVTEIPIPVSFAAAFEGERIRKSDMFAEFGGNRTEAWELVVKKE
                     ATEVEDHKIEIIGPNIDEVDADGVLRLPLAVIVKIAGKNMQEDFEPVLERRFHYFLNY
                     IEGVMHVGQRDMAWVRISKDAFDKGFRLEHIGEVLYAKMLDEFESVVDKCEITIITDA
                     EKVSELKGEAIAKYNARDERLASLVDESVDTFYSCNLCQSFAPAHVCVVTPERLGLCG
                     AVSWLDAKATKELDPTGPCQPIEKGECLDDRTGVWNSVNETVNQISQGAVESVTLYSI
                     LEDPMTSCGCFECICGIMPEANGFVVVNREFASVTPVGMTFGELASMTGGGVQTPGFM
                     GHGRHFISSKKFAYAEGGPERIVWMPKELKDYVADKLNATVKEMTGIENFCDMVCDET
                     IADDSEGVLAFLEEKGHPALAMESVM"
     gene            complement(30735..31541)
                     /gene="acsE"
                     /locus_tag="ACGI59_000027"
     CDS             complement(30735..31541)
                     /gene="acsE"
                     /locus_tag="ACGI59_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase/acetyl-CoA
                     synthase methytransferase subunit"
                     /protein_id="HFG9737842.1"
                     /translation="MEKFMIIGERIHCISPSIRKALAERDPAPILKRAKEQLEAGAHY
                     IDFNIGPAERDGEEIMTWGIKLLQSEFNNVPIALDTANKKAIEAGLKVYDRTNAKPII
                     NSADAGSRFDLIDIAAEYEAMVIGLCAKEGIPRDNDERMAYCQEILEKGLMLGMEPTD
                     ILFDPLCLVIKGMQEKQVEVLEAIKMMTEMGLLTTGGLSNVSNGCPKHVRPVLDSAFL
                     AMAMANGFSSAIMNPCDPELMKTVKSCDIINGASLYADSFLELNEGGFAF"
     gene            complement(31591..32958)
                     /gene="acsC"
                     /locus_tag="ACGI59_000028"
     CDS             complement(31591..32958)
                     /gene="acsC"
                     /locus_tag="ACGI59_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit gamma"
                     /protein_id="HFG9737843.1"
                     /translation="MALKALDIFKLTPKKNCKDCGFPTCMAFSMKVASGAVEVGKCPH
                     MSDDAIAKLSEATAPLMKALKVGAGASEYELGGETVLFRHEKTLVSRNRYAVSFCTCM
                     SDEAVDAKIANMKKVDYVRIGEQMKVEMAVLEYCGDKDAYLKLIDKIKGSGLEVAYIL
                     ACDDAQVVKEAVEVLKDARPMVYGATKENYKDMIEVVKGASLPLGVKAGSLEELYETV
                     ELIQAAGYKELVLDVTGENIKDTYTNAIQVRRTALKEQDRTFGYPSIVFANRLSNSNP
                     MMEVALSSIFTIKYGSIIVIDDISYAKALPLFALRQNIYTDPQRPMRVEPKIYPINNP
                     DENSPVLVTVDFALTYFIVAGDIERSKVPVWLVIPDAGGYSVLTSWAAGKFGGNSISA
                     FIKESKVEEVTNCKDLIIPGKVAVLKGDIEDNLPGWNVVIGPEESMELPKFLKGYQEK
                     ACQTN"
     gene            complement(32989..33933)
                     /gene="acsD"
                     /locus_tag="ACGI59_000029"
     CDS             complement(32989..33933)
                     /gene="acsD"
                     /locus_tag="ACGI59_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit delta"
                     /protein_id="HFG9737844.1"
                     /translation="MAFKMSTQKYSGKISEVEVGIGEKAIKLGGENVLPFYSFDGEVG
                     NSPKIGIQISDVYPESWTDSYKELYKDVANCPVEWAKYVEANTQADFICLKFDGSDPN
                     GLDKSVDECADVAKAVIEAIKLPLVVAGSGNHEKDGKLFEKLAQTLDGHNCLFMSAVE
                     DNYKGVGASAGMAYAHKVGAESSVDINLAKQLNVLLTQLGVKGENIVMNVGCSAVGYG
                     YEYVASTMDRIRLAAFGQNDKTLQMPIITPVAFEVGHVKEAIAPIEDEPDWGCPEERT
                     IAMEVSTAASVLVGGSNAVILRHPKSIETIKELVNALA"
     gene            complement(33953..34723)
                     /locus_tag="ACGI59_000030"
     CDS             complement(33953..34723)
                     /locus_tag="ACGI59_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase accessory protein
                     CooC"
                     /protein_id="HFG9737845.1"
                     /translation="MGYNIAVAGKGGTGKTSLTGLLIDYLVKDKKGPVLVVDADANAN
                     INEVLGIEVEATIGEIREEVNQREKLGNAFPGGMTKAQYLQFRLNSIIEEGEGYDLLV
                     MGRSEGEGCYCFVNGILREQVNKISGHYKYLVMDNEAGMEHLSRKVTRHVDTLLLVSD
                     CSRRSIQAVARIRDLAEELKLSVGRILLIVNKVPNGVMNDGVKEEIEKHNLELIGVVP
                     MDELIYEYDSTGIPLVNLPEDSKSKVAMKEIFAKLELK"
     gene            complement(34779..36164)
                     /gene="lpdA"
                     /locus_tag="ACGI59_000031"
     CDS             complement(34779..36164)
                     /gene="lpdA"
                     /locus_tag="ACGI59_000031"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFG9737846.1"
                     /translation="MKIVVVGGGPGGYVAAIKASMLGADVTVVEKRRVGGTCLNAGCI
                     PTKALLASSGVLNTVKEAKDFGIEIDGTIKPNFTAIMERKNKVVNQLISGIEFLFEKR
                     GVNLVNGFGKLIDKNTIEVTKDDGTVETIKADKIILANGSVPVVPRMFPYDGKVVITS
                     DEVLGLEEIPESMLIVGGGVIGCEIGQFFRALGTEVTIVEMVDQILLNEDKDVAKQLL
                     RQFKKDKIKVITGIGVQTCEVVDGKAVATLSNGKVIEAQYALVCVGRRPNLDNSGVED
                     IGIEMERGKVVVNEHLETNVEGIYAIGDIIDTPFLAHVASKEGIVAVENALGKTKVVD
                     YRAIPRCVYTEPEVAGVGKTEKQLEAEGVEYNVGQFDFRGLGKAQAIGHFQGFVKVIA
                     DKETDKIIGAAVVGPHATDLLTELSLAVHLGLTVEQVGDAIHPHPSLSEGLMEALHDV
                     HGECVHSVPKL"
     gene            complement(36208..37089)
                     /locus_tag="ACGI59_000032"
     CDS             complement(36208..37089)
                     /locus_tag="ACGI59_000032"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HFG9737847.1"
                     /translation="MSLLRETLESGKFAVTTEMAPPKGTDLSHLIECAKPLVGRVHAA
                     NVTDFQSAVMRATSLATCKLLKDAGLEPVIQITGRDRNRIAIQGEMLSAGVFGINNLL
                     ALTGDHTSVGDHPQAKGVFDLDSVGILQTAETLMAGTDMAGNKLKGSPDFYLGASVTP
                     EYSPIEVQLLKMKKKIKAGAKFFQTQALYDINTMRKFRELTRDMDCKVLAGIVPLKSP
                     GMAKFMTANVPGIFVPDEQIERLRAAGKENWVSEGIKMAGELIKQLKEEDLCDGVHIM
                     AIGAEENIPAILDAAGL"
     gene            complement(37124..37798)
                     /locus_tag="ACGI59_000033"
     CDS             complement(37124..37798)
                     /locus_tag="ACGI59_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HFG9737848.1"
                     /translation="MIISENKPLEEVLGYLKDFDKLVLVGCNQCAATCKSGGEEEVLK
                     MKETLEGEGKKILGYVMLDPACNLLKSKKDLKALKEETKEADAVLSLACGDGTQTIVK
                     NLKDKPVYPANNTLFIGEVQRVGEYEEACKACGDCELGWTGGICPVTMCAKGLMNGAC
                     GGAKNGKCEVNSENDCAWIKIYERLEAIGQLDNLAEIRPPKDYSKQNNPRSLSAKKKK
                     EAAANS"
     gene            complement(37835..38707)
                     /locus_tag="ACGI59_000034"
     CDS             complement(37835..38707)
                     /locus_tag="ACGI59_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HFG9737849.1"
                     /translation="MEGMSTKGQIIKGKPVADKISEELIKEVDLLIKEGINPKLTIVR
                     VGARSDDLSYERGALKRCQNIGITTEVLELAEDITQEEYIDVLKRVNDDKNVNGILCF
                     RPLPKHLNEEVIKYVIAPEKDVDCFSPINSAKVMEGDKSGFPPCTPTAVVEILKHYNV
                     DLKGSKVTVLGRSMVVGKPVSMLLLSEHATVTICHSKTKNLSGVAAEADVLIAAIGRA
                     KMVDESFVKDGAVVIDVGINVDEEGNLCGDVDTNAVLDKVSMITPVPAGVGSVTTSIL
                     AKHVVKACKLQNNK"
     gene            complement(38799..39431)
                     /locus_tag="ACGI59_000035"
     CDS             complement(38799..39431)
                     /locus_tag="ACGI59_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HFG9737850.1"
                     /translation="MKIADKTCVDFVEVLSSKEAVPGGGGAAALVGAIGMALGSMVCN
                     LTIGKKKYAEYEESVKEILSKAGKLEKDLLKMIDDDAECFLPLSKAYGLPKETEEEKR
                     IKAETMEKALKVACEVPLNIVRVCYEAIKLHEDLVDKGSRLAISDVGVGVQCLRAAIL
                     GGQLNVVININSIQDKEYVNKVKTEVDKLVEDGVKICDEVYAKVEKALGK"
     gene            complement(39536..41212)
                     /locus_tag="ACGI59_000036"
     CDS             complement(39536..41212)
                     /locus_tag="ACGI59_000036"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014355320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HFG9737851.1"
                     /translation="MGFKSDIEIAQEAKPQDIREVAKKLGLGEDDVELYGKYKAKVDY
                     NLLKRETGKKAKLILTTAINPTPAGEGKTTTTIGVADAFAKLDKNVLVALREPSLGPV
                     FGVKGGAAGGGYAQVVPMEDINLHFTGDFHAIGAANNLLAAMLDNHIHQGNALRIDPK
                     KITWRRCVDMNDRQLRNIVDGMGKKGDGAVRQDGFDITVASEIMAAFCLASDISDLKE
                     RLGNIIVGYSYEGEPVTARQLKANGAMAALLKDALKPNLVQTLEGTPSFVHGGPFANI
                     AHGCNSVIATRMAMHFADYVITEAGFGADLGAEKFLDIKCRMANLKPDAVIIVATVRA
                     LKYNGGVAKADLNNENLEALEKGLPNLLKHVENITQVYGLPAVVAINRFPLDTEAELK
                     LVEDKCKELGVNVALSEVWAKGGEGGIAVAKEVLRLLDEEENNFRFCYEDDLSIKDKI
                     NAIATKIYGADGVDYTPEADKEIANLEKLGFTKVPVCMAKTQYSLTDDQTKLGRPTGF
                     RITVRQATISAGAGFIVALTGEIMKMPGLPKVPAAEKIDVDENGVIAGLF"
     gene            complement(41461..42240)
                     /locus_tag="ACGI59_000037"
     CDS             complement(41461..42240)
                     /locus_tag="ACGI59_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFG9737852.1"
                     /translation="MKIAITGKGGVGKTTFSSMLSRMFAEDGYRVVAVDADPDANLAL
                     ALGFPKEVYESIVPISEMKKLVSDRTAASVGSFGKMFKMNPKVDDIPENFCKEYNGVR
                     LLTLGTVDSGGTGCVCPEHVLLKRLCSHLILQNKDVVVMDMEAGIEHLGRGTAQGVDA
                     FIVVVEPGERSLQTYRKVKKLGHDIGVNKVFVVGNKIRNKEDEEFIIQNLEDGESLGF
                     IYYNQDVIDSDRANQSPYDSSETTKEQIKAIKDKLMSLKDK"
     gene            complement(42550..44469)
                     /gene="cooS"
                     /locus_tag="ACGI59_000038"
     CDS             complement(42550..44469)
                     /gene="cooS"
                     /locus_tag="ACGI59_000038"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_function: GO:0043885 - anaerobic carbon-monoxide
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006091 - generation of precursor
                     metabolites and energy [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic carbon-monoxide dehydrogenase
                     catalytic subunit"
                     /protein_id="HFG9737853.1"
                     /translation="MDEKMLTIDLNSQKMIAKAREEGVETMYDRKEGFKAQCGFGLQG
                     VCCRICGMGPCRISPKTPRGLCGADEHTIVGRNFARMVAGGTAAHSDHARDIAHTLAL
                     ADPNGNYKIRDEAKLITLAKEWDVETEGRDIYDVAHEVAEIALMEFGKPFGTLRFIKN
                     APEPRQKIWKEYAIEPRAIDREIATIMHSTHIGCTGDIDSLIHMSLRTSMADGWGGSM
                     IGTRLSDILFGTPVPRRTEANLAVLEENKVNIILHGHEPALSEMIVLASEEPDLVALA
                     KEVGADGINLAGMCCTGNEITMRHGVKIAGDFHQQELAIITGAVEAVIVDVQCIFPAL
                     ARVADCYHTKFVTTSPKAKITGSTYIEFREEQALDDAKAIVKEAILNFKNRDKSKVLI
                     PELKSGATVGYSVEAVVNQLDRVVNSHIDPAGTVKPLTDCLKSGVLRGAAGVVGCNNA
                     KGVSNEAHVTIMKELIKNDIIVVTTGCGASAAAKFGLMESDAAEKYAGKGLATVCKLV
                     GIPPVLHMGSCVDISRILDLVGAAANYLDMDMCDLPVVGIAPEWMSEKAVAIGCYVVA
                     SGIDTYLGIMPPIAGSSRAVDILTSELKDKVGATFTVNTNPKELAATIIEDIEKKRVH
                     FEALVEEKMAEKAEA"
     gene            complement(45076..45987)
                     /locus_tag="ACGI59_000039"
     CDS             complement(45076..45987)
                     /locus_tag="ACGI59_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HFG9737854.1"
                     /translation="MIKKLDDILEQLKGGEKIVLSVAAAHDKEVLMAIKDAVERNIIT
                     PILVGNEGKIREISKEIGFDLSGIKIVDKDDIKECAEIAVKLVSSKEADFVMKGLLDT
                     SVILKEVLNKDYGLRTDSLLSHVMVYELEKYHKLLITTDGGMNIAPDYEQKAKILKNS
                     IKAAKALGMETVKVACLAAKEKVNPKMQATVDADMLAKACKEGEFGENVIVEGPLAFD
                     LAVSKEASEIKGFKSEVSGDVDIILVPTIEVGNGIGKAFTYMADSKSAGIIMGAKAPI
                     VLVSRADSHESKLYSIAYGAIVAKNMK"
     gene            complement(46103..46681)
                     /locus_tag="ACGI59_000040"
     CDS             complement(46103..46681)
                     /locus_tag="ACGI59_000040"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HFG9737855.1"
                     /translation="MNINEFLTHINQRKPVSSKSEIHEFMVKLSNEAMKITSKLNSSY
                     HEPEEVRSLFSELIGKKVDESFFMFPPFYTDCGKNITIGKNVFINSSCHFQDQGGIEI
                     GDNTQIGHNVVLATLNHGIAPEKRGTTYPSPITIGKNVWIGANVTVVPGVTIGDNAII
                     AAGAVVTKNVAENTIVGGVPAKLLRVIDEIPE"
     gene            46907..47830
                     /locus_tag="ACGI59_000041"
     CDS             46907..47830
                     /locus_tag="ACGI59_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFG9737856.1"
                     /translation="MEIRVLRYFLAIAREESISGAATFLHITQPTLSRQIMELEEELG
                     VKLLVRGNRKITLTNEGRLLHKRAEEIISLVDKVELEFDNPNEIISGDIYIGSGETEA
                     MRLIARTAHHFHQKYPNVRYHLFSGNADDVTEKLDKGLLDFGILIEPANIQKYDYAQI
                     PATDVWGLLMRKDSALASQSTINPKDLCNIPLIISRQTLVDKAISKWLQDSNEKLNII
                     ATYNLVYNASLLVEEGLGYALCLDRLVNTTENSNLCFRPLSPPLEAKLNIIWKKHQIF
                     SKAAEKFLLQIQKDFSSIMLDTSKLDTPKEK"
     gene            complement(48002..49144)
                     /locus_tag="ACGI59_000042"
     CDS             complement(48002..49144)
                     /locus_tag="ACGI59_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFG9737857.1"
                     /translation="MDFKIEKYLLKKAEELAFITIKHGGEFKLKSYEVPKGGLDVPIK
                     NEVLVKGIKEKTAQDKLNSMSIADAMIYIIGIDSKFKNNAEYEKFLNALSKDIDLDLK
                     SYMGYMSRKYFEIGEHTDSLIYIKAFITMYPDDLDAMYNYAIVCQEIAKQYQKDMDDK
                     AMNAFLLEAMAKLEKVIDVDENFALGYYHLGYHYYNQGQYLKTKLTWEEALRLGLDAD
                     LVAEVQENLGKMDFKVQYEEGYTLVFQGKFKEGLEKLLPLEEEHMDWWNLLFMIALGY
                     KGMGEIEQAKMYLEKILIIKPNQVDTIVELGLCEAYKNNLDKAIEYFEQAAKIKEDPE
                     ILCNLGMAYLNNGDIDDATYYIERAYELNPQDEITIACLRELRINK"
     gene            complement(49162..50916)
                     /locus_tag="ACGI59_000043"
     CDS             complement(49162..50916)
                     /locus_tag="ACGI59_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HFG9737858.1"
                     /translation="MKILLTTLNSKFIHTNLAIRYLKEFVRDLIEVNMKEYTINNDLD
                     YILKDIYKNEYDIILFSTYIWNVGDIVKLCDNLKKIRPNTKIALGGPEVSYDSYEAMK
                     KYDFVDYILYGEGELIFRDLVLHLQGKMEINDVNGLVYRQGNEIIVNKPMELLQDLDE
                     IPSPYENLNPKEYENRIVYYESSRGCPFNCQYCLSSTIPGLRYFSLDRIKSDLKALID
                     ARVSQIKFIDRTFNANRKVAMEIMDFLMKNDNGYTTYHFEVTAYLIDDKMLEFLADCK
                     EGLFQFEIGVQSTNEKTLDAVGRRDDFKKLSHVVQTVASYRNIHQHLDLIAGLPYEDY
                     KSFENSFNDVFNLGIEHLQLGFLKMIKGTGMRKVADEHGFKYKDYAPYEFLYNNYISY
                     EETLKLKDIEDILERYYNSKNFVLSMRYIIGRFYKKSPFKFFEVFAKYFDENGYFDLA
                     QGKNQLYKILMDFYNEKINIDNDVFNDILKYDYISLGKTSNIPQFFNKLDVDDFKNRC
                     HVFLQDNDNLSTYLPSFVDKPAKHIIKYVHFEPFRFNVVDLKNDINTEIREVENVVLF
                     EYDDKKIFEKSKTHKVEI"
     gene            51052..51258
                     /locus_tag="ACGI59_000044"
     CDS             51052..51258
                     /locus_tag="ACGI59_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9737859.1"
                     /translation="MELIEKVSINSLSKRDLLLIIKSLEYTNENTQISDFIDLRNNIV
                     KELCFLTDTKEKDFLDYLEKNSTL"
     gene            complement(51263..52186)
                     /locus_tag="ACGI59_000045"
     CDS             complement(51263..52186)
                     /locus_tag="ACGI59_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9737860.1"
                     /translation="MNDELLKKVYTSSFSSASRALKDLTIINTKSLIDDKTQRCVYID
                     ENRLRDELIYYRFYGEKIGNINFLNILLPLILSNTNIQKSEDEVIKLIKKYVIYFKKE
                     ELLFDYLLGSVVYNSIMHNLINNSKIEYVELLQSIKDKIIGFSIELDKSDVVKFQMAR
                     IKAIQLIDKYIDLKSEDYDEESILLNVLNVLYDVYMEDRTVENEGINSIKKSILSILG
                     EDSKLNEDNIDFIFSMSEYVVKLRKYKIGVKAYNKSIDPRSLIRLEEGNTIIDPIFNQ
                     ITVMSKTFNDNILSIKINSKSGIYILKFKKV"
     gene            complement(52440..54140)
                     /gene="argS"
                     /locus_tag="ACGI59_000046"
     CDS             complement(52440..54140)
                     /gene="argS"
                     /locus_tag="ACGI59_000046"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFG9737861.1"
                     /translation="MQDFKVAISNCLKEKIEDLSKEEIEALIEVPPNKDMGDYAFPCF
                     KLAKVFRKAPNMIASELAESIEPSGEITKVIQLGGYVNFFVNKSQLAETVIKKVLDEK
                     ENYGHSDFGKDKTVIVEYSSPNIAKPFHIGHIRTTVIGNALYKIYDSQGYKTIRINHL
                     GDYGTQFGKLIVAFKKWGEKEVVESNPIPELLKLYVRFHDEAEQHPEMEDEARAWFNK
                     LENGDEEAQELWQWFRNESLKEFNRVYKLLDIEFDSLAGESFYSDKMNRVIELLEEKN
                     LLKESKGARIVDLEEYKMPPALITKNDGSTLYMTRDLAAAIYRKETYDFDKCIYVVGS
                     QQNLHFQQWFKVIELMGYDWAKDLIHVGFGMVALEEGTMSTRKGRVVFLEDALNQAID
                     KTKEIILAKNPNAKNVDEISKQVGVGAVVFQELSNSRIKDYTFSWERTLSFDGETGPY
                     VQYTHARCCAVLRKAEVEVTSDIDYSLLADEDSAEVLRVIESFNKNILLALKKNEPHI
                     VTRFMLDLAQAFNKFYHDNPILVENLEIRKARLALVLATKQTLENSLKLLGMHAPERM
                     "
     gene            complement(54602..55015)
                     /locus_tag="ACGI59_000047"
     CDS             complement(54602..55015)
                     /locus_tag="ACGI59_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HFG9737862.1"
                     /translation="MILVSACLIGINCKYSGDNNDNEKVKEYLKGKEFTLVCPEQLGG
                     LSTPRPPAEIVDGKVITKDNKDVTENFVRGANETLKIANLYVCSEAILKEGSPSCGCN
                     LIYDGSFSGKKIPGKGITARLLEEEGIKVKSEKDL"
     gene            complement(55083..57461)
                     /locus_tag="ACGI59_000048"
     CDS             complement(55083..57461)
                     /locus_tag="ACGI59_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HFG9737863.1"
                     /translation="MNEKSLRVLEYNKIIDLLKKKASSSLGLKYIENLVPNTDFVEVK
                     SMLEETSEAQSIIIKRGSVGLEGIHDIEDKVKRAYIGASLDPGSLIMIADTLRVARRL
                     RNSLSSSDEEDFNYPIIQSLSNSLYVYKDIEDQIYNAIISEVEISDNASSTLRDIRRR
                     IAQKNQSIRSKLNSIISSTTYQKYLQDAIISLRGDRFVVPVKSEYRSQVAGIVHDQSS
                     SGATLFIEPMTIVEMNNELRQLKLGEQEEIERILSELSAMVGEVSEDLISNQEILGRL
                     DFAFSKGKLSIQMRGIEPTLNEDKYLNIKNGRHPLLDKKKVVANTIYLGRDFHTLVIT
                     GPNTGGKTVTIKTVGLFALMTQSGLHIPADYGSSMCVYDNVFADIGDEQSIEQSLSTF
                     SSHMTNIVSILQNVTADSLVIFDELGAGTDPVEGAALAIAVLEDINSVGAKCIATTHY
                     SELKNYALTKPGVENAAVEFDIETLSPTYKLLIGVPGKSNAFEISRKLGLSDYVISRA
                     KEYINTENIALEDVLQNVEKNRIKAVEDREEAERLKEEIEKLKVEYDEKLEKLVSQRD
                     KMIEKAKSEAFSIIRQAKEEVDIIIKELRSLEQERASKEKNRKIEELRKELTSSMGSL
                     QPTVKSMIVPKVSNKEIKDLKPGEEVKVITLNQNGSVVSVDKKRKEAVVQIGIMKMTL
                     PFKSLQKTRKDVSTNVTKSTRNIIRSKSGSVKNEVDLRGLNLEEAIMEVEKYLDDAYV
                     AGLESVTVIHGIGTGVLKAGLQDILRRNRHVKSQRGGQYGEGGAGVTIVKLK"
     gene            57780..59231
                     /locus_tag="ACGI59_000049"
     CDS             57780..59231
                     /locus_tag="ACGI59_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HFG9737864.1"
                     /translation="MGNVLEGLKPESVFKNFEKISQIPRGSGNEKGISDFLLSFGKNL
                     GLETIQDESLNIIIRKPATKGYENCPGVVLQGHMDMVCEKEKNVEHDFLKDPIKLRID
                     GDMIYATGTTLGADNGIAVAMGMAILEDNTLEHPALEVLVTVNEEDGMNGADALDPSL
                     IKGQYILNMDSEEEGYLLVSCAGGKTCVVSLPVEYKEVKGDKQGLLVEVTGLLGGHSG
                     MEIVLQRANANKAIARVLSVLNVDYELASVDGGTKHNAIPREAKCVIAVNKADVESAK
                     KQINDILTAFKHEFTTSDPGMTYSVAETSVDKVLTKDCKEKVVQMSCLTPHGVQSVSL
                     DIEGLVESSTNFAIIETKESTIEFLTSVRSSVMSIRDEIADRIRLLAQALGANYDLIA
                     QYPAWEFKKGSKLEKICSETYEKLTGKVPTVMALHAGLECGLLLDKLPHAEAISIGPD
                     MFDVHTPNEHVSIPSVANVWDYVIEILKSMNQY"
     gene            complement(59435..61948)
                     /locus_tag="ACGI59_000050"
     CDS             complement(59435..61948)
                     /locus_tag="ACGI59_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFG9737865.1"
                     /translation="MEDCVVNLKKLLLLIIVITSIFTSCIFSFGINAIGNDNRTIRVA
                     YPIQKGLTEIDEQGNYFGYTYEYLLEIAQYTGWNYEFVQVPGEINESITKLMKMLENG
                     EIDIMGGMLYNEPMQKIYNYAGNSYGVANTVLQALYENTDIDNIMSSQDVKKIRIAVI
                     ENSDTRLKELDEYCKMNLMSPELVYCKTNDEQLEALKSGKADVLLNVSMNPLEGVRTV
                     AKFSSKPFYFITPKGSTNLTQKLNSAINNIEQKDPYFSTTLYEKYFTPENNQMFLSDE
                     ETDYIKETEKLKVGVLLNKPPFQNIDKDTGELRGISIDLLNHICDKTGLEFELVPVKS
                     QEDLENMTKKREIDIVAGMIYDYEYSREHNIVMSRPFLSTQYTMMINNKLNENSLDGK
                     RVALAKGINYYGQFEENIVWYDNLEECIEAVNKGYADYTYGEGYSMQYYMNQPKYKNV
                     RLVPQTYKAKSICFGIIKKDKQELLNIFNKAIISISSEKMQSIVYQNTTYRQDFSLLY
                     IMQQNPIETIFIVASLLICIIGFMAFGLHTRTSMNEKISLELKKHTQLYEMTSEYFFE
                     YDYKQDKLIISMPEKGIETYYGLDKNDKESENGNIENERDKIRKEYNKKFFDVIKSQE
                     NGTTEMYSIMLDDTFHWIRVTSKIIYNDRKIPMYVIGKICNIDSEKEEKNRLLDKAQR
                     DSLTNIYNAAYIRKLTAQNLLNLSTEKNGALLIIDIDYFKSINDTYGHLLGDKVLIDV
                     AKVLDESFAKNDIVGRLGGDEFSVYINDIINKEELINKCNDVYNHIHLIKLPNGESLS
                     ISMGVVIAKPGQEYDELYQIADNALYDSKRQGRNRFKIV"
     gene            complement(62171..62992)
                     /locus_tag="ACGI59_000051"
     CDS             complement(62171..62992)
                     /locus_tag="ACGI59_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFG9737866.1"
                     /translation="MIDINLEKCVGCGMCESDCLVNAIKVKDDKAKVKNILCINCGHC
                     MAICPTDAIEMQGFDKNEVIEYNRESFELEPEKLLNFIKFRRSIRQYKDIEVEEEKIK
                     NIVEAGRYTPTGGNRQPIRYILVKEKLKEVKELAIQGLYNLALDTDDNDPVRSIYKNT
                     FKKMYKRYKENGNDSLFYDAPLLMVVVGDMSLGGSAYVDGGLAASNMELMAYSQGLGI
                     CYNGFFVMASNVEPKIKELLGMSENEAVITSFILGYPDVKYKRTVNRNTAKFEVR"
     gene            complement(63510..63986)
                     /locus_tag="ACGI59_000052"
     CDS             complement(63510..63986)
                     /locus_tag="ACGI59_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFG9737867.1"
                     /translation="MKLTTICYIEKDDKTLMLYRNKKKDDIHEGKYVGVGGKFEQGET
                     PEECVIREVKEETGLTLKSLSYKGLITFPKFKDEEDWYMFLYFSDEFEGELSEKDLND
                     CKEGNLIWVDNDKIFDLNMWEGDRLFLNWAKTGNIFSAKIVYDNGKLKDYNVSFLD"
     gene            complement(64020..64598)
                     /locus_tag="ACGI59_000053"
     CDS             complement(64020..64598)
                     /locus_tag="ACGI59_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFG9737868.1"
                     /translation="MREEALEILFKYLETDRMRKHCYAVEAVMRELAKRLEPEKEEEW
                     AVAGLLHDLDSDIVGRKPGEICEGHATTTVELLKKENFGDEEMYRAILGHHDGMGVTR
                     TSLMEKAIYAADPITGFITAIALVYPDKKLTSVKTKSVIKRMKETRFASNVNRDAMRS
                     IEDIGIPFDEFAELSLNAMKNISDVLESAENN"
     gene            complement(64702..67284)
                     /locus_tag="ACGI59_000054"
     CDS             complement(64702..67284)
                     /locus_tag="ACGI59_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFG9737869.1"
                     /translation="MKKIELLAPVGSFDSLKAAVQNGANAVYLGGKDFSARASANNFD
                     RNELIEAVKYSHIRGVQVFVTVNTLIKQYEIEDFVEYVKFLYDIDIDALIVQDIGMAR
                     LIKKLLPDFELHASTQMVAHSLEDVKYLQSVGFDRVVLARELNVDEIKWICENTTVDI
                     EVFVHGALCVCYSGQCLMSSMIGNRSGNRGRCAQPCRQKYELIDINTGEVVKSNGDYL
                     LSPRDLSTIEDLDKIIEAGVLSLKIEGRMKKPEYVATVVSGYREAINEFVDKKKISIS
                     EEIINNLYTIFNRKFTKGYILGEVGKDIMNSNRPNNQGLYIGKVLDYNRKNKRLRVML
                     ENTLKKGDGINLGGGTIGRIIKDGEITTIAHKGDLIELDFVGEAKRNQLIYKTSDSEL
                     MDRVQATFQQDKEFIKTNIRAKVSIKLGKKPELFISDFCGNKSVVEGDKIVEKAIKVA
                     LSKEKIETQIQKLGNTPYKLKEIEIDLDEGVSMPISVLNQMRRDCIELLDEERIKIKD
                     RKFKKNRLKYQPNRVNKQKKLDTVPKIRVKVKNLEQLEAVLNYNVDLIYYEDINTLSK
                     ALQMVSSISIDSSISMDLYTSSSLGIPKELMGSNTNNIYTLSNTSKIDTLNKNHSNTA
                     VTDKKLIYSAPRIVRNKEYKQFEILDNIKGINKVQIGNWGSMEYFKKKDLNIDCYLNA
                     FNSESINHFKEEGASTVCLSQELNLNEIKQTLKYTDVEIEAVIYGYAPMMVTEYCPMG
                     VVVRDCKKDKRVAKCKESSYALRDFTDASYRITQDIFCRSTIYNSKVTCMLDNLYELE
                     EIGIDVFRIDFTVEDSEMVKKVIEAHIEVLSNNYKLGKRATDLYKKLDEIGTVAGHYY
                     KGVE"
     gene            complement(67854..68537)
                     /locus_tag="ACGI59_000055"
     CDS             complement(67854..68537)
                     /locus_tag="ACGI59_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HFG9737870.1"
                     /translation="MTSKSQMSESFFLCSLLAITGGFLDIYTYVVRGKVFANAQTGNI
                     VLFGLNIAEGNVKESFYYLIPILAFMLGVFIAEKIRKYFTNNKTKIHWRQVIIIIEMI
                     TILIVSFIPRGRFNMFVNVAISFICSMQVESFRKVNGNSFATTMCTGNLRSATETLFH
                     YIHTKNIFMIKKSLQYYAIIIFFISGAILGTFFTKIFVEKSILICFFILAVVFGIMFI
                     NKDDSFNEN"
BASE COUNT        21450 a        12809 c         7509 g        26925 t
ORIGIN      
        1 agttaaattt ataagcttac ttgttataaa aataactttt ataatttatt tcaaaaaaaa
       61 tattgatttt tgtgtcgcat tttgttataa taaattaagg ttatccccct ggtaacccta
      121 gttagttaat atatctattc ccaataccct ttcataagga tatattttat atttattaaa
      181 aatcggatta gtaacggaaa ttaattcggt ggaaaaaaag agcttacccc gagctctttt
      241 ttttctgcaa aaaatcgtaa aaataaagtt aatattaata taactttaat tttaaaatta
      301 atattaacca tcactaaaaa aggatgtatt gaatttctaa tatcaataac atccttttta
      361 tatattatag cacttttgct aaaaatgctt ttgttctttc atgttttgga tttccgaaaa
      421 cttcttctgg agtattatct tcaagtatac ttcctccatc tataaaaata actctatctg
      481 caacttcctt agcaaatccc atctcatgag ttacaattgc cattgtcata ccttctttag
      541 caagttcttt tataacatcc aaaacttcgt ttaccatttc tgggtcaaga gcagatgttg
      601 gctcatcaaa caacatcata tctggttcca tagcaagtgc tctagctatt gctattcttt
      661 gcttttgtcc tccagataat tgtgatggat atgaatcttt tttatctaaa agaccaactc
      721 tactcaacaa aatttctgct tttttttctg cttcttcctt agtcatcttt tttaatttaa
      781 ttggtgctaa tgttatatta tctaaaatag tcttatgtgg gaataaatta aagttctgaa
      841 acaccatacc tatattttgt ctaacctcat caatattagt ttttttatcg accaaatttt
      901 ttccttcaaa tattatttct ccacctgtag gtatttctag taaattcata catctaagaa
      961 gtgtactttt accagaacca cttggaccta ctataaccat tatttcgcct ttagcgattt
     1021 ctaagtctat attttttaat acatttaaat ctccaaaaga tttacttaaa ttttttattg
     1081 taatcatatt atcactttcc tttttactat tctatgtttt ccctaaatgc taaacatgct
     1141 ctcatctagt tagacacaga tagtttcttt tctaataatc caactaatct tgatagagta
     1201 aatgttataa caaagtaaat tgctgctgct actattaatg gctctaatgc tctaaatgct
     1261 gcatttttta ctatatcagc agtacgcatt atctcaacaa ctcctattac agatattatt
     1321 gaagattctt ttacaagtgt aataaactca tttgctaatg aaggtaatat attttttata
     1381 gcttgaggta ttattatata tctcatagtt gaccaataat ttaatcctaa tgatctactt
     1441 gcttccattt gtccattatc aactgattgt atacctgatc taagtatctc tgccacgtat
     1501 gcaccagagt ttactgataa tgcaaatgca ctagctataa attcacttgg tataccaaac
     1561 aacataggga atcttattcc taattgaggt aaaccaaacc atactatata tatctgtact
     1621 aataatggtg tccctcttaa aacctcaata tagattgatg atattgctct taataccttg
     1681 ctctttgata tttttgccat acagataatt atacctatta taaatccaaa gcataatgcc
     1741 acaagagaaa ttgatactgt cacacctgtt ccttccaaaa aagaagtccc aaatctactt
     1801 aaaaaactaa aatctaaact cataatttca tctcccacat aactttctta ctaatttgaa
     1861 gcagcttcag tagatgcttc atttaaaaat tcttctatct tcttgccatc ttgtaattct
     1921 tttatctttt tgtttaataa ttctataaaa tctttgttat tgtcagattt tttgattgct
     1981 gctgccatac cttctgttac atctttccca acttcagtat ttgccatttt tattccatca
     2041 tattttttaa catttattaa agaaactgct tcattagtaa caactgcatc tatgtttcca
     2101 ttttttaatt ctgtaattaa atcaggtatt gctttcaaag attttgtagt tgtcattttt
     2161 aaagtgttaa ctacatattc ttcttggata cttcctgctt gaactccaac tctaagtttc
     2221 tttaaatcat cttcagtttt tactttgtct atatctgcat ctttaactat aactgcattt
     2281 ctacttaaat aatataattc tgaaaaatca acactctttt ctctctctgg tgttggagac
     2341 atacctgcaa gaacaatatc aacttggtct gcatttaaag cacctattaa tccatcaaat
     2401 gacatatcct taatttcaac ctttacacca agctcttttg caaattcttc agccaacatt
     2461 atatcgaatc cttttattga atcttttcca tctactacct tatgaaattc aaatggagga
     2521 aattctgcac tagtaccaac aactaatttt ccattgtctt ttatttgttc taactttgtt
     2581 ttttctcctc caccactaca tcctactagc gataatgtca atcctaatac taatcctaaa
     2641 cttaataatt ttttaaatac tttcatttta actacccctc tcttttaatt ataatttccc
     2701 tcagatttta atacttaaat gtttcgagtt acaaaaaaag tctctcagtc tttaatgact
     2761 aagagacgac tttaccgcgt taccactctt attgataaat acttatatta ctttcaaaat
     2821 ttgtgactgt tagcatttaa gctgtatgga tacttttatt tttaaatcaa aaaagtccct
     2881 tagctttaca gctaagagac gaattatatc cgcgttacca ctctttttga taagtaagta
     2941 cttatcctct caatccctta aggcgggtaa accaattatc atactaaagt ttctgataat
     3001 ctcctccaga gctctcttca catagacttc attgctaggc ttacaccaac ccctaactcg
     3061 ctgtaaatcc atatatgcta ctttctctat cattggattt gtttccttaa attgttaata
     3121 caattgtact tccttataat atttttgtca ataagttttt tattttttta ataattttta
     3181 cttgtaaatt atctaaatac tactgtttat atttattgat attagttttg taattataaa
     3241 tagtatattt catattttta gatatttctt aaataatact aaataatatc tttcacattg
     3301 ttgctttttc ttaaacaata ctaaataata tctttcacat tgttgctttt cttaaattac
     3361 actaaataat atctttcaca ctgttgcttt ttctttaata atactaaata gtatctttca
     3421 cactgtttct ctttctttaa taatactaaa taatatcttt cacattgttg ctttttctta
     3481 aattacacta aataatatct ttcacattgt tgcttttctt taataatact aaataatatt
     3541 ttccacattg ttgctttttc taaacaacta taaataatat attaaaccct tatttctttt
     3601 tctaataagt taaataatac attttacaca tatctatttg tcttgcacat aataaataat
     3661 acattatatt tctatctaaa tttagattct aaattaaata gtataaatta tattcttcaa
     3721 taaacaagtt ttcaaatcca agattcgctc tctatcttta aactttattg taaacataat
     3781 tccatctttt ttaattattt ctccttcatg aaatttttta tggtatacct tatcccctac
     3841 accaatactg tcaatttctt ttttcgttgg agattttata tcattaataa ataatgattt
     3901 atcaactttc ttaccatact tatttaaagt agagcttata cataactttt cttcagctct
     3961 tgtaatagca acatacataa gtcttctttc ttcttctaat tgttcttctt ttttctcttc
     4021 cttacaaata tcatatgatt tttcatgagg tattgttcct tcatttacac ctataatata
     4081 aacatttcta aattctaatc cttttgcact atgcatagtt gtaaatatga caccctcagt
     4141 ctgcttattc ttgttatttt ccgttaactc agtctttact ctatcaatat gctctaaaaa
     4201 ctctgtaaca gtattaaaat tagttgctga actctcaagt tcatttaata tctctacaag
     4261 tccattagtc tttatctttc tgttgctaca ataatctaaa acatatctat cataatcaag
     4321 actagttcta atataagata ttgcattttt aggatttaaa gtatttaaat aacttaaatc
     4381 tatttccaaa tcatttattg ttttaacttg tttaggatga agattacact tatttattaa
     4441 tgctgttata aagtcttttt catccttaac catatttata ctgtctttgg atatatatct
     4501 aaaaggcttg tttataattc tcaaccaatc tttatttgat tttggattaa tacctattct
     4561 taaataggct agtatgtctt gacttgccca atggtcatat attgtaatta ctgagtcttt
     4621 tactacaaat ggaatcctca tatccataaa tacatctact aatgctcttg actgtatatt
     4681 agttctataa attactgcaa aatctgaata ctctacataa tctttttgta tttcgtcaat
     4741 tatttccctg gcaatatata aggcttcttc ctctgaatca tgcggagaaa tataggtaac
     4801 acttcctcca tccccctgac tacacttaat gattttttca tatctatttt tatttttgtc
     4861 gataagtcta tttgcagtat gaactatctc acttttagac ctgtaattta tatctaatat
     4921 tattttttta gtattattaa agtattgttc aaattcaagc aaaaaatctg gtctagcacc
     4981 tctaaatcca tatatacttt ggtcttcatc tccaactgca aatatattgt ttaatgggct
     5041 acaaattaac tttaaaactt caaactgtac tttatttata tcttgaaatt catctatcaa
     5101 tatatattta tacacatttc taaccatttc tagtgcagac ttattgttca aaagtaaata
     5161 ataagttctt ataagcatat catcaaaatc tattttatta actttagatt tttgttcttc
     5221 gtataagttg tatactttta aaaactcatc ttttgttaat acttctgaat taaattcatt
     5281 tttatccata agttcatttt ttacatagga aatttcattt ataacttgtc ctatattctc
     5341 atcatcatca gcattctcta tatttaaact ttttaggatt gccttgattg tcattcgctt
     5401 tgacttttca tcaaatatac tgtctaagtt gtatctttca aagtatctta gaattttaaa
     5461 aaatacagag tgaaaagtac cataggttac cttattaagt cttatgtcat cacttaaatt
     5521 caaagctcta tttttcattt ctattgaaga tgccttggta aaacttattg ccaagattct
     5581 tgttggtgct atgtttttgt ttacaaccat attggctatt ctatatgtaa ttactctggt
     5641 cttacctgaa ccaggccctg caagtatcat gcaaggccca tctatatgct caactgcttc
     5701 taattgattt tcatttaact ttcttatatc tatatttgtt gtgattatta gctccaccac
     5761 cttgatttat aattatatta ttttcttttt aattataaac taaaagttca taaaaataaa
     5821 tttaattttt cctctattaa accacatcat tcctattttc atgacatatc ttgagttctt
     5881 ttttaatttt gtcgcttata tcttcaggta taaatgatgg tttactaata tagtagcctt
     5941 gcatataatc tactccaaga ttaattagtg tttccatttc accttttgtt tctacacctt
     6001 ctgctatcac tttaatattt ctttcgttag aatatgagat aagatttttt aatattttct
     6061 gtctgttttc atctttatca atacctctta caatagacat atcaatcttt acatagtctg
     6121 gcattatata taatagtaca gcatctccgt tgtaacctgt cccaaagtca tctaaagcaa
     6181 tttctatatt ccaatggtct atatatgctt tctttttctc aataaaatct tcattaatct
     6241 tatcattctc tgttatttct agtactactc tatttaaata ttcaccatat aaatcttcaa
     6301 tttcctcaaa atctactttt gacaaattat tattaggaat agaatttaag aacagtttgc
     6361 aatcgccaaa tttttctttg tattttttaa atgatttaag tgctttaaac catgtaatac
     6421 gctcaatttg atataattta gactgtgaac gagctaatct aatgacatct attggcgaac
     6481 taaaagtttt tattttaggt ctcataagtg cttcatatgc aaaaacttta cctttacaat
     6541 ctacaatagg ctgaaatgca tactcaacta attgaccatc aattaacttg tttaattctt
     6601 ctctattact caataagaat gaatccttat tatatatatt aatatcaaat tcactaactt
     6661 cacccttgat tgtattttta atttgataca tagcaaaatc tgcgtatctc attaattcat
     6721 catagttggt tgagtcatca gggtaccagg aaattcctgc tgatgccctt attttaaatt
     6781 ctgttccatc atgaagtttt aataaggtat tattcatctg tttcttaaca gaattaataa
     6841 tatctcttat actatcttta ctttcatatc catatataaa tacataaaac tcatcacctg
     6901 aaagtctaga tactatacca ttccatgata taaacttctt aagtatgtct gcagcacacc
     6961 taatatattc atcaccagca tcatgtccat atgtatcatt aatatatttt agattatcta
     7021 aatcccacat aataaatgct gctatcttaa gttcatcttt attcttaaat ttttcttgta
     7081 tctgtgaatg aaaagctctc ctatttaaaa gattcgttaa aatatcaaaa tctctttcat
     7141 attctatctt ccttttctca ataaattctt ttgttacatc aacaacaaca ccaagaacct
     7201 ttagctcatc ttcaaagata ttcattctta tccacttaag ttttttatct ttagattcaa
     7261 acttatatat atatgtattt tctgtttcat aactttcttg caaatattta tctagtcccc
     7321 ttaatttttt caggaactta tttttttcca tatatccata ttcttcaaat ttgatttcag
     7381 tagtaaacac gctaaaaaat tcttttgtac aaaaaacatt ctctgcgttt ttgtataact
     7441 caaaagctcc aattggcata tctaccattt caataatctg tgataatttt gacgaagaat
     7501 ctgcaacatt tgcactgagc aactctatag ctgaagataa ttcatctatt tcagatatat
     7561 ttatcttatc aagatttact ggttttgatg gattactgtt tctaactttg ttggctaaaa
     7621 gaacaattgg ttttgtaaat aatcttgatg caatataaat acctattaga cctattaata
     7681 aagaaattgc tatagatatt ataactaatt tctctaccct ttttgaaaat gacaaaagac
     7741 ttttttcttc aataataccc aaaagagccc aacgttcatt ttcaaatgga gtattggagt
     7801 tatataaatc taagtattgt acactagcat aaatgttgcc attaccacat tcattatctt
     7861 tatttttaat cttatatata tttttatacg attccttctg ctctaaatta gaatgtaaac
     7921 catcccaaaa tttttctttt aatgagtatc catttgaaat tacattttca aaggccatat
     7981 cattatttct atctacacct aaaatataat tacccttttt attcccattt atctctgagt
     8041 atggaagtaa ttctcttaaa taatttacac ttaaatctat tccaagtact ccatatacag
     8101 ttccatcttc ataaataagg ggaactgtat atgtcataag cctatcacct gcatttgtca
     8161 ataaaaactt acttccccag tatcctaaat tagaacttga tacatttgga ttatcaattg
     8221 cagctctaaa tggtttataa aaaaaatcat ctccatcttg ctcattttca cttttaaatg
     8281 aaaacttagg ttcccaataa ctgtccatag atattccaaa acttcttgct atatctgatg
     8341 aacctctctc aattaacaaa tcagaattat catttggatt aaacttaggg tctaaatccc
     8401 ttatatacaa tcctgtccta cttatttcac ctttactatc tattgactta tcgttaatat
     8461 ttttgtcact taggattata aaagctcctg taacagaatt ttttctcaaa agatatatta
     8521 aatcatttga tatgcttttt ataatatctt tgttaatttc ttcattgttg cctacatcct
     8581 caattaaggc atttttttca tttaatactt tttttacaga ggaattaatt gtagcttccg
     8641 tttcacttgt atttgaccat ctttgtaaca tttcattttg tatataattt ttacgattaa
     8701 ttactctttc atttaagatg tcaaacgaat tatcatttaa tttcttaata gttcctcccc
     8761 ataaaattgt ggcacaaaat aaacttgttt gaattagcat aacaataatt aggggaaata
     8821 tcatcttttt cattattgaa ttgttttttc ttagattgtt ttccataccc attccaccta
     8881 tacctttgtg ctcgtcaaca cactaactgc caattcatct atttttttat agcattttca
     8941 agctcagcct taaaactttc aaaccaagtt ttaaaatttt tatcattatt aaattgtgct
     9001 actgcatcct tcttagaagt tccacttttt actaattcat ttattttatt tgaatcttct
     9061 ttagctttat caagcattga atattcaagt atttgtctag ctttagttcc accatcaaat
     9121 gctttattag tatataaatt acaactgtta atctgttcaa tagatgaggc tattgaacct
     9181 tcaactttat cagacaaact caaattatct ttcttcataa cttcttttat aacatccaaa
     9241 tcattcgttt ttttctttac tggtaagtat ccagaagcaa tagagaattt tatattaggt
     9301 tctttatctg taaaccattt taaaaattct actgatgcat attcttcttc tttagtagat
     9361 tttgttacag ccattcctgc accttgttgt acagcatatt ttccttctcc tttaaaacgt
     9421 ggtgcttgaa aaacagatgt ttcaatatta taactctcat tatcatttag cattactcta
     9481 tctggaaagt atgctgctcc tgaactagaa cctacaagag ctaatatatc acctgttttt
     9541 gcatcatcag aacgaaactt accataagaa gcaaaatatc catttatata tggtacatag
     9601 aaattatccc atagtttttt cataactttt tcatcaatat ttagcgtaac tttaccatct
     9661 ttaacagaaa aaatttctac tcctagttgt ttacttccta taataatata atttgctaaa
     9721 gcatctcttc caaaaaatgc tttaccatca ttaggctctt tcgttaaact atctgtccat
     9781 tcataatatt tttttgatgt ctctactaat ccttcaattg tttttatatc atttatattt
     9841 gaatttgtag cttttgcaaa tttatcccaa tcagttttat ttaacatcaa aatttcagtt
     9901 gattttgcaa ttgggaaaag tttaaactca tccttagaat ttattctgcc ttcttctaca
     9961 tattcgtcta tgtattcatt taattcttct ttacttatgt acttatccat atctactagt
    10021 aaccctaatt tatccatttc atatgcagta tctgagtatg ctgcaaatat atttggtaca
    10081 tcttcagaac caactttttt atttgcagaa tctaaaactt tctctgataa atcaactaca
    10141 ttaccttgac tgaatgcttc aacaataatt cctttttcag cacctacagt gtcattaaat
    10201 tccttaacaa gcttatcaaa agccattttc tgctgtccat tataataatg ccaaatttgt
    10261 attgatgtcg gattttctgg gtctaactta taattatctt ccttctttaa actacatcct
    10321 gtaagtaata aaacaatcat tgatgtgata attaattttg caaacttaca cctattcata
    10381 aaatctactc cttcaaatta taaaatttat aatagcatta aaactgtaac ttcactattt
    10441 atattaatta tagaacgttt attgatatat ttaaacagga tttacaacat atttaaaagc
    10501 aattaagaaa catactatta caaatataga aaataaagct tttacataat ttttatttct
    10561 aaatgcacca aatgacacca atccttgttg aatagctact gtaattacta aaagtatcat
    10621 tattatatta agttgaacca gtttaaataa gtaaagcaat gaaaacacta ctagaagtac
    10681 acataataca atactagatt tattaataat atcatcaatc ttatctactt tcttagattg
    10741 cttattaatt ttatttaatt tttctttatt taaattctca acttttttat tatacctttc
    10801 tcttgtttta tctttatatt tagtattttt atttttattt cccatttatt ttccatcctc
    10861 tataaatttt ttaattgagc ttatacatta tttaaatatt atatatttaa gataccatat
    10921 attcctacaa aaaggtaact tactatgtat ctatttttga ttttcgcata aaaaagccat
    10981 ctcctttaga attaattctg tattgagaca gctaatttaa ctatttatcc tttgaaacat
    11041 tttctacatt atcatattta ctatcaacta aaactatatc aactcttcta ttttttgacc
    11101 taccttcttc agatgaattt tctgctattg gtctaaactc accatatcct acagcagata
    11161 atttatctgg tgctatacct gcattgtcaa taagaagttg aacaacattg gttgctctca
    11221 taactgataa atcccaattt gacttaaact ctgaattttt tatagacata ttgtctgtat
    11281 ggccttctac tctcatgtaa ctattcattt catttaacat cttacctatt tgaataatct
    11341 tatctctaga attatccttc acaatagctt tccctgtatc aaatagtatt gtatctttaa
    11401 ggcttattac taatcctcta tcttgaactc ttaatgatac tgaattagcc ataccatttt
    11461 cgtttaaata cttctgaatt tcttcaccaa tttttttaaa cttagcattt tgtaaagtat
    11521 ctaaatcatt acttcctggt tcaactactg gttctatctt gctattacca ccttcaataa
    11581 ctccagaaga accaccaaaa gcagaattta gagattgaga taattgttta tatttctccg
    11641 catcaacatt actcatagaa tacattatta caaagaaaat cataagtaat gttatgaggt
    11701 ctgaataagt taaaagccat cgctcattgt tttcttcttt ttcatcttca tcatacaaca
    11761 taaacattcc atccttttat attattcagc tctaccacca atttcttcaa gttcaattag
    11821 ttcttttgaa tttaaaaata tttttaattt ttctgtcatt aaattagaat tatcaccctc
    11881 aagtatggat aatattgctt ctatcaccaa aagtctttca cttacttctt gttgatttat
    11941 agccttaagt tttgatgcta ttggtaacca tattaaattg gcagaaccaa caccatacaa
    12001 agtagccaag aatgcaacag aaatttgagg tcctaatgca tctggattac ttgaaagatt
    12061 acctaaaata tgtactaatc ccataactgt acctattatc cccagtgtag gtgcatatcc
    12121 acctgctgaa tcaaaaattg ctattccttc tttatgtcgt tttgatgtta tctgtatctg
    12181 tgattccaat atatttctta tagtaactgc atccactcca tctaccataa gttcaagccc
    12241 ttttctaata aaagggtcta attcatcatt ctcacttatt tctttgtcaa tactaagaag
    12301 cccctctttc ctagcctttg ttgcaatttc tttgaaatag attatatttt ctatgttatt
    12361 aggcttttta tttttaaaag ctaataacat tatctttata gactttttaa acacagggaa
    12421 tggagtcgaa gctccaacag ctcctatagt tccaccaaac acaacaagtg ctgatgaaaa
    12481 tacaaataat gctccaacat gtcccccatc aataacaaaa gcaactacca aagatgctat
    12541 ccccactatg aaaaaaatta aagttgtcat ctattccctc ctttctttag ttatacatat
    12601 ctaatcattt atgacattat aaaaagacta tctttaataa aaatagcctt taataattgg
    12661 cggagaagga gggattcgaa ccctcgcacc ggttacccag cctaatccct tagcagggga
    12721 tcctcttgag ccacttgagt acttctccat atctctatac taataaatta tataataaaa
    12781 tcatatttta gtcaattgtt ttataaaatt tgagttattt tgaattatta aacatctatt
    12841 tttacttttt tgcttaaatg taagttttaa atatggtaaa attatcttgc ataacactta
    12901 taatattatt tgataaatat acatacatta acaaatatac ctaaactctg acttaattgt
    12961 aagttatgcc aaaactaaaa taatactagt ccaaggagga tattttatgt taccacagga
    13021 aagatatgag aagattttaa gtattctcaa atcagataat ataattaaaa taaatgacat
    13081 agttcaaatg tttgatatat caatagaaac tgcacgaaga gatttagccc atatggagag
    13141 tatcggtctt ttaaaaagag tttatggtgg tgctatacca gttatagcaa gtagcgtaga
    13201 acttaactat gttaaaagaa aaaaaataaa ctcaaacgat aaaaaacaga ttgctattaa
    13261 atgtagtgag tttataaata caaatgatac tatttttatg gatactggaa ctactgtact
    13321 tgaagtttca aaaaatttga aagataaaaa gaatctaact gtaataacca actcaatttt
    13381 agttgcaaac gaacttatga atactaatat aaaagtatat gtgcttgggg gctatcttag
    13441 agaaactgaa ggttcttttt ctggtccgct tacactgtca gctattgaac aatttaatgt
    13501 tgataaagct attttaggcg ctggaggtat cactcttgaa aatggaatct ctgactatca
    13561 cttagaagaa gcacttgtta gaaaaaaaat gattgaaagg tctagaaaat ctatagtagt
    13621 tgtcgaacct cctaagtttg gtataaattc atttgcatca gtagctccta tagaacatat
    13681 agatactatt attacctctg ctagtataga ccataatata ataatagagt tttctcaaaa
    13741 aggaatacct ttagtcatag ctgataatta gcttatatga ttgattttta gttccttgat
    13801 gtaataaatt aaaattaagg agccaaataa ttatttattt agctccattt atgtctaaac
    13861 tatactttat attttaatta tgtaactact ttcagttagc ttatcatttt tagttatttg
    13921 attgccattt tatttaattt ataagtttta gttattcgat tattttttaa ttgtctgtct
    13981 ttttaattta tttttagtta tttaaactcc tcctttaaaa taatttaaat ttgacttaag
    14041 tattgtttat tctacactgc atctttcttc attttaatac tttgttcttc ttccaaggtt
    14101 aaatcttttg tcataagtat agcaagtgca attgccaata ctcctccaac caattttcca
    14161 attacaacag gtgtaatcat ctctggaact actcctgctg taaatcctaa atggtccccc
    14221 aaagctgctg tagcacaaac cagccaagca gtatttacta ctttcccctt tggactcata
    14281 tctttcatca ttttatatac tggaatacta ttggctaaag taaaaactaa tcctgctgca
    14341 ctagtggcat ccatacctaa ttttccaccc actattgtaa gtggtttatt aagaacttta
    14401 actaataaat gtaatattgg aaaagtacct aataacacga ttgcaatact tccaattact
    14461 tgaatacctt ctcttatagg tgtcatgcct tttataagta ctatacctgt tatttcttga
    14521 aatgctgctg ctcccaaacc tatcgttatc aatatagata taaattttcc aaaaattaat
    14581 gacccattta tcataagagt agaattaaat tttaaaccta ctgccaatat tactgccaat
    14641 attactgcca atattatcac tggtatcata tttataatta ccattgataa gttaaatcct
    14701 gctattaatc ctcctataat acctccaaag ggtattgtta tcaagcctat cagtaaccct
    14761 tttgcaaaga atggtctgtc ttcatattct ataagaccta gtcctactgg aattgaaaat
    14821 actaatgtac atccaagcat ggatgacaca accaatcctg caagcattcc tgcctcttta
    14881 ttgtgtgcaa gttccatagc caatggatac cctcccatat cattcgctaa aatagttgca
    14941 aacatagatg ggtcagctcc acagagattg aagattggta ttattatagg acctagtaca
    15001 tctgaaatta ctggagctaa acaaactata ccaaccattc ctaaagccaa tggacccatt
    15061 gaattaaacc cttcttcaaa ccgctcgcca agtccaaatt tatttccaat tattctatct
    15121 aacgctccta aaagcactcc cacagccatt atgtataata taattttatc aaagggcatt
    15181 tttaattcct cctatacatg taaaatactc tattgtaact taattagcac acaccttttt
    15241 ggtatcgttt tcccaattca tagctctttt tactgcttgg ttccatccaa gtaatatttc
    15301 ttctcttttt tgagtagtca tatttggaat atattttttg cttattgtaa attcacttct
    15361 aatttcatca atactgttcc agacaccaca agaaagacct gccatatatc ctactccaag
    15421 tactgtcgtc tcaggatttt ctgcaatttc aattggtata cctaatatgt cggattgaaa
    15481 ttgcattgta aagttatttt tagtaattcc accatctact ctcaacattt ttattttttt
    15541 ccctgaggct aagttcatag aatcaacaac atctttgact tgaagtgcaa cagactcaag
    15601 agtcgctctt actatatgct cttttttagt acttcctgtt atcccaacca ttgtacctct
    15661 agcatattgg tcccaataag gtgctgctat acctgcaaat gctggtacaa aatatacacc
    15721 tccagtactt gatatggaat ttgccatgtc atcagtttct gaataatttt ttataactcc
    15781 aatgccatct ctaagccatt gtattgcagc acctgctata tacactccac catcataagc
    15841 atatgtaact ttatctccta ttttccaagc aacagttgtc aaaataccat tacctgctat
    15901 atttagttcc tcacctatat tcatatatag gaaacatcct gtaccataag tatttttaac
    15961 cattcctggt tcaaaacatc cattgccaaa taaagctgct ggttggtcta ccattgaagc
    16021 agtaattttt atagaaaccc cacaaaatgc ctccttatct gtagttccaa aatcaccact
    16081 cgtaggcatt atatctgcta aaatacttct aggtactcct ataatgtcaa gcaactctct
    16141 atcccaatcc atatctctta tattaaaaag cattgttctt gctgctgtag atacatcagt
    16201 taaaaaagat tttcctcctg tcattttcca tattaaccaa ctatctaatg ttcctgcaag
    16261 tacttctcca tttttaattt tttcttttac accttttaca ttatctatta cccatttaat
    16321 ttttgtacct gagaaatatg ggtctataat taacccagtc ttatctatta tcttattttc
    16381 taaacctggt atattcttta aattttccac ataatcataa gttctcctgc actgccacac
    16441 aatagcattg taaagtggtt tacctgtatt tttatcccat aacattactg tttcaccttg
    16501 attatctata ccaattcctt ttatatcact ttcattgatt ccattttcaa aagcctgttg
    16561 taatacatta ttactcacag tttttgtatt attccaaatt tctatagggt catgttcaac
    16621 atatccagat tgtggatata tttgagtatg ttccatataa tcgctaaaaa caaactctga
    16681 tttttcgttt atcaatactg ctcttgttcc tgttgttcct tggtcaattg ctaatacata
    16741 tttcacttta aatgtcctcc tttattttca gaattaataa attctttttt attttttgaa
    16801 tatataatct attcaagtta tttatatttt atatcatcat gtggatttat gcaatattca
    16861 ttacaatatt ttttgttatt tttaattata gtgattgaaa aagtattttt attgaaatat
    16921 tgttgcaata tttattattt tattctataa tctagttaaa ataacacata atattttaat
    16981 atttacacat aatcagtcat tcaaaaattt aaatttaact ttttaaggag gctttacaat
    17041 gaaaaaaaca tatctattca gtcctggacc agtaatggtt acagataaag tcagaaattc
    17101 acttcttcac tatgatatat gccatagagg aaatgagttt atggatttat tcaaagatac
    17161 acaacaaaaa atcaataaac tatataatgc aacttctaat tactactcat taattgtatc
    17221 tggctctgga acatctgtaa atgaatgtgt actatcttct atctttgaca aagaagatgc
    17281 agttctgcta gttagtaatg gtgtttttgg agaaaggcta gaagaaatat tatctactta
    17341 taatgtaaaa acatataaac ctaattttga atgggcagaa tacccggacc tagacatttt
    17401 agaaaaatac ataattgaaa actctgatat aaaagtaatt gctatggtgt tccatgaaac
    17461 tagttcagga atgataaatc cagtccctga actaggaaaa cttgctaaga agtataacaa
    17521 aatatttttt gtagatgcaa taagtgcctc agcaggtgaa tatattgatg tggaagagtt
    17581 taatatagat ataataacag gtgttggtgg caaagctctt ggagcatttc caggttctgc
    17641 atacctttgt gctaaagaga gtattttaca aaatattaaa gaaaatcaat gtaagaatgt
    17701 gtatcttaat ctttataaac actataaagt tgctaaatca tcttctcaaa caccaaatac
    17761 acctaatgtt actttaatat ttgcccttaa tgaagcttta actgagtttt tggaagatga
    17821 cagtaaaata gaaagataca aagaatgttc tgctatttta aggtatggta tggaagaatt
    17881 gggtctaaca tttttacttc cagataagta tatgtctaat acagtaacat ctgtatttct
    17941 acctaaagaa atcaatgtaa atgagtttat attagggcta gaaagggaaa gtggctatgt
    18001 tgtctatcca ggaaaaggca aattcctaga tgctaacata ttccaagtag caaatatggg
    18061 agaaatatat ccagaagatt gttataaatt cttagatatc ttaaaatcaa aattggagat
    18121 aaaaaaagtt atataacagt ttgtttgtaa aatcctacaa tatatcagta taaattaaat
    18181 agactgattt ttttaaattt tcctataata aattatattt tatatttttg tataagggtg
    18241 aactataata tgtaaagttc acccctattt caaatctttt tccttcaata taatcttttt
    18301 cttttaattt attttatatt tttgacgcaa tcgtttttta ttcaatcatt ttatattctt
    18361 gatacaatca ttcttacact taattatttt ttgtttttga cacaatcgtt ttttgttgaa
    18421 tcattttata ttcttgatac aatcattctt acacttaatt attttttgtt tttgacacaa
    18481 tcgtttttta ttcagtcatt taatattttc aacacaatca ttcttacatt ctattattgt
    18541 ttgtttttga tacaactgtt tttattcagt catttaatat tctcaacaca attacctttt
    18601 cattcaatca aagtaatctt taagcattat ttttcaagct catcaaagta attatatacg
    18661 ataacctcac ctgtctcttt atctgtagtc tttataatgc ccttagctcc atttacctct
    18721 tcttttgtaa attcatcatc taacacaact ttgaatttat cttcctttcc ataaccttgt
    18781 tgaaattcat ctctcaaaat tgcatttttt tctgcttttg gcttcaactc aaattcaatt
    18841 ccagatttca tttcatcaaa ataatttaat actacaacct cattattcat tttattagtt
    18901 gtctttataa tccctttagc tccatttact tcttcttttg taaattcatc atccaatgtt
    18961 atattaaatt tgtcttcttt tccatattcc tcttgaaaat ccctcttcat ttcattgtat
    19021 ttagtttcaa actctttact cttgtttaat tccatagctg atacatttgt caatgatgct
    19081 ccaattgcca ttactgttaa tgccattact ataccacttt taaatttttt tactttcata
    19141 tcttacctcc taaattaaat gttctcaatg aaattatata aagaaaatcc taagttttac
    19201 agtttcattc ttgtaactaa aattactatt atgtaaccaa actttttgct tcaactattc
    19261 cttaaaagtt gcttttttga ggaatagttg aagttaaata attagatttt atctatattt
    19321 atagatttaa aatcaaattt atccatataa ataaatactt atttttgcaa aataaaaatt
    19381 tttaattact ccaatccttc ttatttactt aaaatcctat gataagaaat ttaaaagtct
    19441 ttaccttctt taacaataat ttatttactt ttaacataca aaatatagag agtatatcaa
    19501 aactatttta ctgaatattc taaaaataag taccccctat tagtatatat atttacatat
    19561 taaaaaaaag ttttaaataa agttatattt tttacaacag ttttgtaatt aaaaacatat
    19621 tgaaggtaaa tttaaaggta ctatatacta taaatatgtc gacacaataa atttaaaaag
    19681 gagaactaaa tatgaaaaaa agattaataa taatgatatt gtctgttgtc ttagtactat
    19741 caagtatact tacgatattt gcatattcaa atattaaata taataataac aaattgatat
    19801 attctaatat gatagataaa aaaactcaaa atagtgtaaa ggaaatccta aaagaaaata
    19861 aaattaatga aaaagacata gatacattta ttaaatcagt caacaattat aataaattgc
    19921 aagttaaaat acttcaaaat aatataaata tatctaaatc tggatattct tcaatcaatg
    19981 caaaacaagt tccatataac ctagaaaaat tgcaagataa ttgggtgaaa aaattccctg
    20041 attatatgga tgtaaattgt agaataactg cttttagact ttttaaagat tttataaatt
    20101 caaataagaa atttactgga gactctattg atttgaatgt cgacttggac actataatga
    20161 ataataaaga tgctaaattt agcaccaaag atgtagaaaa atttatcaac ttcttttctg
    20221 caataccagc taaagataca gatgatacaa tcaaaattgc agaacaaata aaaaatgaat
    20281 ggaaaaaaag aaaaatatct tttaaaaata ataaaaatat atctattata aatggatttc
    20341 ttcgttatcc agaaactaaa aatgtattta taggtcatac aggcatatgt attaaaacta
    20401 aaaatggcat tctattttta gaaaaatacg gtgttacatc accttatcaa gttactaaat
    20461 ttaaaaataa agaagatgta aaaaattata tgtttaatag attgaagatg tcagaaggtg
    20521 aaattgaatt acctgaccct ataataatgg aaaacgataa acttatgaaa taataacttt
    20581 aaacgcaaaa tagtatttat aattactaag tcaatataat agagaaggtc tatagatttg
    20641 tgtaaataca agcttatcta caatcttata taagaatgtt tatataaata agatagatta
    20701 attctaagtc ctattattct attgaatttt attattattt tatttagatt ctattgttat
    20761 tttacttaga acttattatt attttgttta agttctatta ctattttcaa tcatataaat
    20821 ttacaaaaga ctgtacataa atttatttta tacaaactta tctacagtct tttgtgtgaa
    20881 tatatctaaa taatatcgta tttatttatt tcctctgcac agtgagaatc agaacctaaa
    20941 actaatggaa tatcgtattt ttttaccaag tctaataagt atccatctat ataaggctct
    21001 ttgcagtact cttttctatt tccagaaaca ttataatcaa gttcataacc tttttcttta
    21061 attaacttta ttaactcttc aagaatttta tttcctttat aatcgtatgg aaatacctga
    21121 ttaaactttc taactaaatt taaatgacct attctttttg gtttatgtat acctaaatct
    21181 gaatttacag cttttatcaa tgtttcataa tacttattat aaacagcttc aatacttcct
    21241 aatttgtcga ttaaattttg aaactcctct acactgtaat ctacacaata ataatcatca
    21301 tcaatcttaa ttatatgtac tgataaaaga gaatcctcta attcctttcc atatttattt
    21361 aaattctcct ttatcccttc ttcgtatcct tctataaaat ccacttccga cccaacattt
    21421 atcttgattt taccctcata cttttctttc aacttcttga catcattaaa atactttggt
    21481 aaatcttcaa tactcataca actatcattt tcaggagcag ggtctctaaa tccatttgca
    21541 tacggaaaat gttctgtaaa tgtcatttct gttattccaa catccattgc tctttgtaca
    21601 tatttttcaa aatcatcctt acttccatga ggacaataag gtgaatgtat atgaccatct
    21661 ttcataaata attcactcct tttttaaaga aaaaatctaa tataaatgca taaatctttt
    21721 aacttcaaaa taatattttt aatcatgcta atttttatta tatctaatat tagagtaatt
    21781 ttaaactgcc ttttaagtat cttatcatta gtactatttt caacttaaca ttaatattat
    21841 tttttaactt aacattaata ctatctttaa atagtaatac tcaatatata attacttaac
    21901 ataaaataac tcaaattgct tgatgaggct tttgcagtat ttaaagaata tataattatt
    21961 taacattaaa taatccaatc ttgtaataaa atttactcat taaagtatat tacagaaaat
    22021 acctcaaaat aaacttttta gttcatgata tactcttttt tgcatgaaat tagatatatt
    22081 tttatcattt caacaatgaa agggaggctt atatccatct ttagacaacc ccttagataa
    22141 aatttttaat aattaaacat cacaaaatat aaaaatcgct tatatcaaac gtataagcga
    22201 ttaattatat ttacatatgt tttttagcct ctaacaacat tttattagct tcttccatat
    22261 actctatagc cttttcaata ctctttgctg tttcttcttt tccaatagct ctagcctttt
    22321 caacccactc tctaaatcct tcttcatgag tttcattgtg atttatccaa tgtactaaaa
    22381 gtatttttaa agttttttca tccttagact ctgatgattc ctcatgacta tgttcgtggt
    22441 cgtggtcgtg agcatggtca tgtggatgtt catgacatat atctccatga gtatgttcgt
    22501 ggcagtggtc atggtcatga tgtccatgat gatatcttac aatctcttta tcaaaaatca
    22561 ttagttattc cccctgttat tttatattaa actctttttt tataaggttt acagaatctt
    22621 ttacaagtag tgctaatggt ttaccttcta atccaactaa ctccacattt tctggcataa
    22681 taggtaaaag tatcttaagt gcctcactgt ctgcaatagc ttcacttata ttacaagtaa
    22741 cttctcccat catagaatta ggtattatta cagaaatagg tgcaactatt atatttgctt
    22801 ttttagctga tactactata gcattttccc ctgtagctcc tttattagca tgagctttca
    22861 tcatagaact agttgctata gaattagtcc ctagtgcata aatttctaca tatgtaggta
    22921 actcttctct caaagaagaa actatctggg ctccaatacc tccacccatt ccatctatca
    22981 ctgctattat catatgaaat tcccctatta attaattttc ttgtattatt atcttatggt
    23041 ccatcaatct tatttcttta attatcccat ctattatctt ttgctctcct aataaatccg
    23101 ttaaataaat ctttccatca caagggtcta tattaaccac attttccata accttttcta
    23161 actcattatt acttttacaa atgaaagctg atgattcaca catataaatc tcctcctaat
    23221 atgattttta ttaaaaatca aaaaaagatt cccttaggta tttcaataca ccaaagaaac
    23281 cttcatgatt tcagtttttg tatattatta taccttaagt atatcacttt aatttatgta
    23341 aagtcaatta aagttaacgc tttttattat actcttcatt cttgtatttt tcatatagtt
    23401 ctttaactaa ttgcttctca taatcagtca aatcgccctc cacaaactca acattaaatt
    23461 gttcagctaa tccctttact atgtttttct gtagaatatc tatgtctatt tttctgccaa
    23521 tttcattttc tattccactt gcttttttag ctgtaaattt catagctctt tctttcaatt
    23581 ctggtgtctt agtttttatt aatttaaaca atttttctac atcaaaatcc agtattattg
    23641 aaccatgttg taataaaaca ccatcttttc tactttgagc acttccaatt accttcttat
    23701 tattaatagt aacttcatat gatgcatgtg cattaaaaca tgcagctgat aaattttccc
    23761 tacttattct ctcgcctcta tttaaattat ctgtttctat accacttaga ttcaaccctt
    23821 taactagacc ttcacttata tatctataag ataggttgat gctcttatcg attaaaggat
    23881 tatcttctcc tataattata ctataggtta gctcattatc atgtaataca gctcttccac
    23941 ctgtaattct tctagtatag tctatattca tacatctaca cttatctaaa tctatctcat
    24001 cttccaattt ctgaaaatat cctatactca aacaagctgg ttcccatgta taaaatctaa
    24061 gagttggctt attatgtcct ttcttatatg cagtaaatat agcttcgtca attgccatat
    24121 tcattgctcc ttcatatgat ttattatgta taactctcca ctgattcatg attattacgc
    24181 ctccattttt agtataaata tatcctagaa atcctctata gatttatttc tcttattgct
    24241 aaatctatct cactttgatt taaaggtctt gaatagttgt atatagtact tcctggtctt
    24301 tcattatagt atggtctatt acaatcctta caacctctta tttcaaaagg atagcctttg
    24361 tttatgtctt ttaaaatatc attgtcaagc tttatactat ttaagtatcc atttttaaac
    24421 ttaaaatact cttttggata acctttattt atcatataag acattaactg tacccttcta
    24481 taactttcta tgcttggttg atttatcttt tccattttag ttcctctaac tggagtaaat
    24541 gcaaataaac taatcgttac atcattttcc tttaaataag tatataaatt gtatatatct
    24601 tcatgacttt cacccatacc aactattata tgtgtactta ttttattggg atatgatttt
    24661 gacatttcag ttataaattt taacttttca tcatagttat ttccttttat tttttcatat
    24721 agttctttat ttgctgcatc tattgctatt cctattttat caactcctgc tgaaaaaaac
    24781 ttctttatct gttcatcatt ttctaatttt gcagatagtg aaattggcat gtctatctta
    24841 ccacttatat agtttataaa gtctaaaaca gagttatgtg cttcttcact tgccattgat
    24901 tgaatacata ttctctttat attttttcct ttataagctt ttaaagcatc taatatttct
    24961 tcttttgaaa actctggcca aattactctt gataatttat cttttctagt actactttct
    25021 attgattgag aacaaaaact gcatttattt atgcattttt ctccaatcat tatatatgct
    25081 gtggtaggag gtatatcgct ttttttgttt aatatcccaa gttctattgc tgtcccaact
    25141 gatagtctaa tcataaaacg caacactcct tacactcatt tattgtcata ttcatttcta
    25201 tagccttatt ctttgcagac cttgatggta atactattct attgactcca cacattatag
    25261 ataattggtc tagctctttt ctatacactc ctcttggtct catacaacct agattaattt
    25321 cagtatctgg taaattaatt ctagcttcac ataaaatatc tgctacccct tctaactcag
    25381 gaggttctac attttcatac tcagtccctt ttgttggaat aaggactata aatgtaagct
    25441 tattaggagg atttttttgt aggtattcta taatttcata ttcacccttt atttgtcctc
    25501 cttttaaacc aatacatata tgtggtgcta cttctgtatg ttcttgtatt gtattataga
    25561 cttcaatata atcttcttta ctctttttca tcttataaac ttctcttatt gtctcatcat
    25621 caaacactaa atcaaaagaa actatatcta aatacttaca caactctaca atgctatctt
    25681 tatccataag acctaaatga gcattcaatc tatatccttg ctcttttaag tttttaatgg
    25741 tattaatatg agtatttata gggacatctc cctcataact acaaccgcca ctcaatagaa
    25801 agctagttat attccttgat tgtacttttt cctcatattc ttctattggc accatattgt
    25861 ttaagtagtg accgttgcaa tgggcacatt tcaaactaca ttgattgctt gtagtactaa
    25921 gagctagtgt ctggttagga taagcaaact ctattttatt tccaaaattc tttaatttaa
    25981 tatcaaatgc tattttcatt ttttcagata gattcttatt catagtttat tccttcttaa
    26041 gtacatttta ctcttctagt aatttctttg ctgttgggaa ctgctctata ttcgtatgtg
    26101 gtaagaaaca tgcagaaata aattcatcca tataacttgg atatacacta agttcaacat
    26161 aagttatttg attacctatt tcttcaagtt tattttttgc atctttactt ataagcgcta
    26221 agtaagaacc aacaagtgaa ctattcccta tataagtaaa tttttctcta tcaatatcag
    26281 gaagtaaccc tataattatt gagttttcta tgtctaggtt gtttcctata ccacctgcta
    26341 tataaaccct atctaatata ctaaaatcca tacctaaact atctacaaga gtgtatgcgc
    26401 ctgaatatat agctccttta gctctgatga aattatctat atcaacttca ttgactacta
    26461 tatcattttc taaatcaaat tcttctttaa atgctaatac atactctcct atctcatgct
    26521 cattaaatct aactctctta ttatctaaat ccctgtatat tttacctctt ctatctatta
    26581 cacctttagt aatcatttga cagattaaat caattatacc agaaccacat ataccaactg
    26641 gtgcacattc atcaataatc tttaatgttg gctctaaagt atctttatca ataattactt
    26701 tctctattgc tcctgctgat gctctcattc cacagcttat ccctccacct tcaaaagcag
    26761 gtcctgctga acatgcacag ctcatcatat aatctttatt tccaaataca atctctccat
    26821 tagttcctaa atctataaat aaaacatttt cttcacttga ccatatccct gctgataata
    26881 cacctgctgt tatatctcct cctacataac ttgcaacaga tggagctaag tacacatatg
    26941 cactgtcatt tacaaaaaga ccaacatttt cacccataag ttttggagat tttaaaaatg
    27001 gtggtatgta aggttcttgt cttaagaagt ctgtatacac acccaagaat aaacttgtca
    27061 tagttgtatt tccggcagcc actaatgtaa ctacatcttc cttatctatt ccatttcttt
    27121 catatattgt ttttaatagt ggatttatag tttcctcaac tatagcttta tgaagtgtct
    27181 ctaatccatt tttcttagtt gaatatataa ttctatttat aacatctgct ccatatttta
    27241 tctgtgcatt tccagaagat gctttatcta ctacctcttt tgagtatagg tctactaaac
    27301 aaacaactac tgaagttgta ccaatatcta ttgcaactcc gtataaggaa ttttctttat
    27361 ttccttgttc tatatttatg attgtaagtt tcttttgttt ttgaacatat gttattgtaa
    27421 ccttaaaatc actctttcta aatacagttg gcatttttct aagtatatcc aatctaaaat
    27481 ctatttcatt atatcctagg ttatttctaa catatctttc caatctatct acatcactta
    27541 tattatcatc taaatttggt tcttccattt ctatgtattt cttttttata tttgttttaa
    27601 attgaaggtt atgttcttca attatttttt tagctctttc aaaaatttcc ctatcctctt
    27661 ttttattact accttctatc ttcataccat gcatagaaga tgatacttta gaaggaactt
    27721 caatttctat atctgatatt acctttgtac aacaagctag tatataacct tgctcccact
    27781 catcatctgt tatatgtctt gttttttctg tatcaacttt tccattcaat aattttactt
    27841 tacacttccc acaagacacg tttccattac aaggagcatc tatgaatata tctgcatttc
    27901 ttgcaacctc tagtaatata tccccctcat tacaataaac ctctttattg tttggtgtaa
    27961 aacttacttt tatcatgtta tccctcccaa tttgttctat atatgataaa aggaaagtat
    28021 agctaaattt gcgtgcactc aaattttact atactttccc caattataat aatatcttaa
    28081 tctttaatta ttctaatcca gcctcgtatt ctttatcact taataacgta tcaagttcag
    28141 ccacatcagc tagttttact tttaatatcc agttttcata tgggtcttca tttatacatt
    28201 ctggctcatc ttctaatttt tcattaactt ccaatacctc accattaaca ggagatatta
    28261 aatcagaagc tactttagaa gactcaacta ctccaaaatc tactccctta gttacagtat
    28321 cttctacttc tggtgtttca acaaacaata tttctcctaa ttgatcttga gcatagtctg
    28381 ttatacctat atatacaaca tctccatcaa ttacttttac ccactcatga tctttagagt
    28441 attttaattc tggtaataat ttcatttcga tagaccccca atattatatt attgattaaa
    28501 ttacttaaat catattaata ttatatcata ttattttaaa ttttccatca attacattac
    28561 actttccata gctaatgctg gatgaccttt ttcttctaag aaagctaata ctccttcaga
    28621 atcgtcagct atagtttcat cacaaaccat atcacagaag ttttctattc cagtcatttc
    28681 tttaactgta gcatttaatt tatcagctac ataatctttt aattcttttg gcatccaaac
    28741 tattctttct ggtccacctt ctgcataagc gaatttttta gaagatataa aatgtcttcc
    28801 gtgtcccata aatcctggag tttgaactcc tccacctgtc atagaagcaa gttctccgaa
    28861 agtcatacca actggagtaa cacttgcaaa ttctctgtta actacaacaa atccatttgc
    28921 ttctggcatt ataccacaga tacattcgaa gcatccacaa gaagtcattg ggtcttctag
    28981 tatagagtat aatgtaacag attcaactgc accttgagat atttggttta cagtttcatt
    29041 tactgaattc catacacctg ttctatcatc taagcactcg cctttttcta ttggttgaca
    29101 aggacctgtt gggtctaatt ctttagtagc cttagcatct aaccagctaa ctgctccaca
    29161 aagaccaagt ctttcaggag taacaacaca aacgtgagct ggtgcaaatg attgacataa
    29221 gttacaagaa tagaaagtat caacactttc atcaactaat gaagctaatc tttcatctct
    29281 agcattgtac ttagctattg cttctccttt taattcagaa actttttcag catctgtaat
    29341 tatagttatt tcacacttat caactactga ttcaaattca tctaacatct tagcatataa
    29401 aacttctcct atatgctcaa gtctaaatcc tttatcaaat gcatctttag agattcttac
    29461 ccaagccata tctctttgtc caacgtgcat tacaccttct atatagttta agaagtagtg
    29521 gaaacgtctt tcaagaactg gctcgaaatc ttcttgcata tttttaccag ctattttaac
    29581 tattacagca agtggtaatc ttaatacacc atctgcatca acttcatcta tatttggccc
    29641 tattatttct atcttatggt cttcaacttc tgttgcttct ttcttaacaa caagctccca
    29701 agcttcagtt ctgtttccac caaactcagc aaacatatcg ctctttctaa ttctctcacc
    29761 ttcaaatgct gctgcaaatg aaactgggat aggtatttca gtaactttta tttttattcc
    29821 tctagcttct aatgaagtag ctactatctt atcgtaatct ttttgagtta ataggttcat
    29881 tggaacttca agaactgttt ggtcagttat aactgggaat cctaaagcta ttgctccagc
    29941 acctgcagat acaactaatt cacttaatgg tccaaatgca ttaacaaatg caggaactct
    30001 attagctgtg tactctaata atccgtttaa atcaccagga gttaatccac cgaatattaa
    30061 tgctgctctt actgcaacag atacaacgtg tataacagaa gttacatcat atcctagagg
    30121 tataactctt agctcaagac ccatttttac tccagcttct atagcttggt ctataacttt
    30181 accaactaag aaagttagta aacctttaga ttggtaatct tttataactt ttgctgctga
    30241 ttctgcatct ggacactcac caagaacaac tgcaacacca ggtatatctc ctgtaactaa
    30301 tggtacacca agtgatctga ttatagggtc agttatatga cctgcacatg gctcactata
    30361 tggagcatcc atagttgaat attttaatgc ttcaattact tctgctgcta aagctgtagc
    30421 taaaccagca ttaaaagcat gttctagtaa atgagttcta tttattaaag attttactac
    30481 ttctaaagca ccttctaagt cccctaaagt gttcatcttt tgtcctgttg cagcatatat
    30541 acaaggtaat gaatatgctg tatcagggaa agcaacttta tgctcttttc cattcttttc
    30601 tatagcttca gctaacatag cttgagccgc acctaaagct tgttctgacc ctgtaaagat
    30661 tatattatat agattcattc tatttccccc tttaatattt acacctattt tttattaact
    30721 ataccctaaa aaccctagaa agcaaatcca ccttcattta actctaagaa agagtctgca
    30781 tataaagatg ccccgttgat tatgtcacaa gattttacag ttttcattaa ttctgggtca
    30841 catggattca ttatagctga actaaatcca ttagccattg ccattgctaa gaatgcacta
    30901 tctaaaacag gtctaacatg cttaggacat ccattagata cgttagataa tcctcctgta
    30961 gttaaaagtc ccatctcagt catcattttt atagcttcta aaacttctac ttgtttttct
    31021 tgcatacctt ttataactaa gcataatggg tcaaatagta tatcagttgg ctccattcct
    31081 aacattaagc ctttttctaa tatttcttgg cagtaagcca tacgctcatc attatctctt
    31141 gggatacctt cttttgcaca taaacctata accattgctt cgtattcagc tgctatatct
    31201 attaaatcaa atcttgagcc agcatcagca gagtttatta ttggttttgc attagttcta
    31261 tcataaactt taagtccagc ttcgatagct tttttattag ctgtatctaa tgctatagga
    31321 acattgttga actcagattg aagtaatttg attccccatg tcattatttc ttcgccatct
    31381 ctttcggcag gtcctatatt aaaatctata tagtgtgctc ctgcttctaa ttgctctttt
    31441 gctcttttta agattggagc tgggtctctt tctgctaatg cttttcttat tgaaggtgat
    31501 atacaatgta ttctctcacc tataatcata aatttttcca tttataatgt ccccctttaa
    31561 atttaatatt aatagttagt taatatttta ttagtttgtt tggcatgctt tttcttggta
    31621 tccttttaag aacttaggta attccataga ttcctctggt cctataacta cattccatcc
    31681 tggtaagtta tcttctatat cacctttaag tactgcaact tttcctggaa tgataaggtc
    31741 tttacaatta gttacttctt ctaccttact ttctttaatg aaagcagaga ttgagtttcc
    31801 accaaattta ccagcagccc aagatgtaag aactgaatat ccacctgcat ctggtattac
    31861 taaccatact ggaacttttg atctttctat atcaccagca actatgaagt aagttaatgc
    31921 aaagtcaaca gtaactaata ctggagaatt ttcatctgga ttgttgattg gataaatctt
    31981 tggttcaact ctcattggtc tttgtgggtc agtgtatatg ttttgtctta gtgcaaataa
    32041 tggtaatgcc ttagcataac taatatcatc tattactatt atagaaccgt attttatagt
    32101 gaatattgat gataaagcaa cttccatcat agggtttgaa ttagataatc tatttgcaaa
    32161 tactattgaa ggatatccaa atgttctatc ttgctctttt aaagctgttc ttctaacttg
    32221 tatagcatta gtgtaagtat cttttatgtt ttctcctgtt acatctaata ctaattcttt
    32281 atatccagcg gcttgaatta attcaactgt ttcatataat tcttctaagc taccagcttt
    32341 tacacctaat ggtaaacttg ctcctttaac tacttctatc atatctttgt agttctcttt
    32401 agttgctcca tataccattg gtctagcatc ttttaatact tcaacagctt ctttaactac
    32461 ttgtgcatca tcacaagcta aaatataagc tacttctaat ccactgcctt ttattttatc
    32521 tattaatttt aaatacgcat ctttatctcc acaatactct aaaacagcca tttcaacttt
    32581 catttgttca ccaattctta catagtcaac ttttttcatg ttagctattt tagcatctac
    32641 tgcttcatca ctcatacaag tacagaatga aactgcatat ctatttctac ttactaatgt
    32701 tttttcatgt ctaaataata ctgtttctcc acctagttca tactcagatg caccagcacc
    32761 aacttttaaa gctttcatta aaggtgcagt agcctctgat aattttgcta tagcgtcatc
    32821 agacatatgt gggcatttac ctacttctac agctcctgaa gcaaccttca tagagaaagc
    32881 catacaagtt gggaatccac aatccttaca gtttttcttt ggtgttaatt taaatatatc
    32941 taaagctttt aatgccatta ttttttcccc ctaaccttaa caatttaatt aagctaatgc
    33001 gttaaccaat tcttttatag tttctattga ttttggatga cgaagtataa ctgcgtttga
    33061 accacctact aaaacacttg ctgcagttga aacttccatt gctatagttc tttcttctgg
    33121 acaaccccaa tctggctcat cttctattgg agctatagct tctttaacat gacctacttc
    33181 aaaagctact ggtgttatga taggcatttg taatgtctta tcattttgac caaatgctgc
    33241 aagtctaatt ctatccatag tagatgcaac gtactcatat ccataaccaa ctgctgaaca
    33301 tccaacattc ataactatat tttcaccttt aacgcctagt tgagttaaca atacgtttaa
    33361 ttgtttagct aggtttatat caacagaaga ttcagctcct actttatgtg cataagccat
    33421 accagctgat gctcctactc ctttgtagtt atcttctact gctgacataa ataagcagtt
    33481 atgtccatcc aatgtttgag ctaatttttc aaataattta ccatcttttt catgatttcc
    33541 tgaaccagct acaactaaag gtaattttat tgcttcaatt actgccttag ctacatcagc
    33601 acattcatca actgacttat ctaatccatt tgggtcagaa ccatcaaatt ttaaacaaat
    33661 aaaatctgct tgtgtattag cttcaacata tttagcccac tctactggac aattagctac
    33721 atctttgtat aattccttat atgaatcagt ccagctttca ggataaacgt ctgatatttg
    33781 tatacctatt tttggagaat tacctacttc tccatcaaaa ctataaaaag gtaatacatt
    33841 ttccccacct aatttaattg ctttttcccc tattcctact tcaacttctg atatttttcc
    33901 agaatatttt tgagtagaca ttttaaatgc catatttttt tcccccttaa gcctatttta
    33961 attctaattt agcaaaaatt tctttcatag ccactttaga ttttgaatct tcaggtaaat
    34021 taactaaagg aatacctgta gaatcatatt catatatcaa ttcatccata ggtacaacac
    34081 cgattagctc taggttatgc ttttcaattt cttctttaac accatcattc ataaccccat
    34141 ttggaacttt atttacaatc aaaagtattc ttccaacact taactttagt tcctcagcta
    34201 aatctcttat tctagcaaca gcttgtatgc tacgtcttga gcaatcacta accaataaaa
    34261 gtgtatcaac atgtctagtg acttttctac ttagatgttc cataccagct tcgttgtcca
    34321 tgactaagta tttgtaatga ccagatattt tgtttacttg ttctctaagt atcccattta
    34381 caaaacagta acatccttca ccttctgacc tacccattac taataagtca tatccttcgc
    34441 cttcttcgat tatagaattt aatctaaatt gaagatattg tgcctttgtc ataccaccag
    34501 ggaatgcatt gcctaacttt tctctttgat ttacttcttc tcttatttct cctattgttg
    34561 cttcaacttc gatacctaac acttcattta tgttagcatt ggcatcagca tctacaacta
    34621 atactggtcc ctttttgtcc ttaaccaaat aatcaatcaa aagccctgtg agacttgttt
    34681 taccagttcc accttttcct gcaacagcta tattgtatcc cattttaaga cttccccctt
    34741 aaatctagcc acagagcctt atgtactctg tctgtctatt ataatttagg tacagaatga
    34801 acacattctc catgtacatc atgaagtgct tccattagcc cttcagataa acttggatgt
    34861 ggatgtattg catcccctac ttgttctact gttaatccta gatgaacagc aagtgacaac
    34921 tctgtcaata aatctgtagc atgaggtcct actactgctg cacctattat cttatcagtt
    34981 tctttgtcag ctataacttt tacaaatcct tggaaatgtc ctatagcttg tgcttttcca
    35041 agacctctaa aatcaaattg acctacattg tattctacac cttcagcttc aagttgcttt
    35101 tcagtcttac caacaccagc aacttcaggt tcagtataaa cacatcttgg aattgctcta
    35161 taatctacta ccttagtctt acctaaagca ttttcaactg ctacgatacc ttctttagaa
    35221 gcaacatgtg ctaggaaagg agtatctatt atatcgccta ttgcgtatat accttctaca
    35281 ttagtttcaa gatgttcatt aactactact tttcctcttt ccatttcaat tcctatatct
    35341 tctactccag aattgtcaag gttaggtctt ctaccaacac atactaatgc atattgtgct
    35401 tctataacct ttccattaga aagagttgca acagccttac catcaactac ttcacaagtt
    35461 tgtactccaa tacctgtaat aaccttaatt ttatctttct taaattgtct aagtagttgt
    35521 ttagctacat ctttgtcttc atttaatagt atttggtcaa ccatctcaac tatagttact
    35581 tctgttccta aggctctaaa gaactgccct atctcacatc ctataactcc accacctact
    35641 attaacatag actcaggtat ctcttcaagt ccaagaactt catcactagt tattacaact
    35701 tttccatcat atgggaacat tcttggtaca actggaacag aaccatttgc tagtattatc
    35761 ttatctgctt ttatagtttc tactgttcca tcatctttag ttacttctat agtgttttta
    35821 tcaattaatt ttccaaaacc attaactaag tttactcctc ttttttcaaa taagaactct
    35881 ataccactta ttaattggtt tacaacttta ttttttcttt ccattatggc agtgaagttt
    35941 ggcttaattg taccatctat ttctatacca aaatcctttg cttcttttac agtattcaat
    36001 actcctgaag aagcaagaag tgctttagtt ggtatacatc ctgcatttaa gcaagttcct
    36061 ccaactcttc ttttttcaac aactgttaca tctgcaccaa gcatagaagc ttttatagct
    36121 gctacatatc ctcctggtcc accacctact actactatct tcatctttaa ttcccccaaa
    36181 cctaatataa tctacaatct tgcaatacta taaacctgcg gcatccagta tagcaggtat
    36241 attctcttct gccccaattg ccattatatg aacaccatca cataaatctt cttcttttaa
    36301 ttgtttgata agttcccctg ccatttttat tccttcactt acccagtttt cttttccagc
    36361 tgctcttaat ctttcgattt gttcatctgg aacaaatatt cctggtacat ttgcagtcat
    36421 aaatttagcc attcctggtg attttaaagg tactattcct gctaatacct tgcaatccat
    36481 atcccttgtt aattctctaa actttctcat tgtatttatg tcataaagtg cttgtgtttg
    36541 gaaaaattta gcccctgctt ttattttctt tttcattttt aataattgaa cttctattgg
    36601 agaatattct ggagttactg atgctcctag ataaaaatct ggagaacctt ttaacttatt
    36661 accagccatg tccgtacctg ccattaaagt ctcagcagtt tgaagtatcc ccacagaatc
    36721 taaatcaaac actcccttag cttgtggatg gtctcctacg ctagtatggt ctcctgttag
    36781 tgccaacaga ttgtttatcc caaatacacc tgctgacaac atttctcctt gtatagctat
    36841 tctatttcta tctctccctg ttatttgaat aactggctct aatccagcat cttttaataa
    36901 tttacatgta gcaagagaag ttgctctcat aactgccgat tgaaagtctg ttacatttgc
    36961 tgcatgaact cttccaacta acggctttgc acattctatt aaatgtgaaa ggtcagtccc
    37021 ttttggagga gccatttcag tagtaactgc aaattttcca ctttctaatg tttctcttaa
    37081 taagctcatc tctaatcccc cttttaacct tctaaaaaaa cttttatgaa tttgctgctg
    37141 cttctttctt tttctttgca cttaaacttc ttggattatt ttgcttagaa tagtcttttg
    37201 gtggtcttat ttctgctaag ttatctaatt gtcctatagc ttctagtctt tcatatatct
    37261 ttatccaagc acaatcattc tctgagttta cttcacactt accattttta gctccaccac
    37321 aagccccatt cataagacct ttagcacaca tagttactgg acatatccca cctgtccaac
    37381 caagttcaca gtctccacaa gccttacagg cttcttcata ttctccaact ctttgtactt
    37441 cccctataaa caaagtattg ttggctggat aaactggctt atcttttaaa ttcttaacta
    37501 ttgtctgtgt accatctcca catgctaaag ataaaactgc atcagcttcc ttagtctctt
    37561 cttttagagc ctttaaatcc tttttagact ttaaaaggtt acaagcaggg tcaagcatta
    37621 catatcctaa gatttttttg ccttcacctt caagagtttc cttcattttt aaaacttctt
    37681 cttccccacc acttttacaa gttgctgcac attgattaca accaactaaa accagcttgt
    37741 caaaatcttt taagtaccct agcacttctt ctaatggttt attttcagaa attatcatat
    37801 ctacttcccc cctaaacctt ccacatatat atatctattt attgttttgt aatttgcaag
    37861 ccttaactac atgttttgca agtatagatg ttgtaactga accaaccccc gctggaactg
    37921 gagttatcat tgatactttg tctaaaactg catttgtatc aacatctcca cataaatttc
    37981 cttcttcatc aacatttata ccaacgtcta taactaccgc tccatcttta acaaaacttt
    38041 catcaaccat cttagctcta ccaatagctg ctattagcac gtctgcctct gctgctacac
    38101 ctgatagatt tttagttttt gaatgacata ttgtaacagt tgcgtgttca cttaataaaa
    38161 gcattgatac tggctttcca actaccattg atcttccaag aactgttacc ttacttcctt
    38221 ttaaatcaac attatagtgt tttaatatct caactactgc tgtaggtgta catggtggaa
    38281 atccactttt atccccttcc attaccttag cagagtttat tggactaaaa caatctacgt
    38341 ctttttccgg tgcaataaca tattttataa cttcttcatt taagtgtttt ggaagaggtc
    38401 taaaacataa tataccatta acatttttgt catcattaac tctctttaat acatcaatat
    38461 attcctcttg tgttatatcc tcagctaact ctaacacttc tgttgttata cctatgtttt
    38521 gacatctctt tagtgctcct ctttcataag ataaatcatc acttcttgcc ccaactctaa
    38581 ctattgttag tttaggattt ataccttctt taattagtaa atcgacttct tttattaatt
    38641 cttcactaat cttatcagct acaggttttc cttttataat ttgtccttta gttgacattc
    38701 cttccataca ttcacaactc ccctacaagc aaacttaaat taatttattc tatataccac
    38761 atattaataa catagtatct actttcgtca tttaaaaatt attttccaag agctttttca
    38821 actttggcat aaacttcatc acaaattttt acaccatctt caactaactt atctacttct
    38881 gtttttacct tattaacata ttctttatct tgtatagaat ttatatttat aactacattt
    38941 aattgtccac caagtatagc agctcttaaa cactgaactc ctacaccaac atcacttata
    39001 gcaagtcttg aacctttatc aactaaatct tcatgtaact taatagcttc atagcaaact
    39061 cttactatgt ttaatggtac ttcacaagca acttttaatg ccttttccat tgtttctgct
    39121 tttattcttt tttcttcttc tgtttcttta ggaagcccgt aagcttttga taatggtaag
    39181 aaacattctg catcatcatc tatcattttt aataaatctt tttctaactt tcctgcctta
    39241 cttaatatct cttttacact ttcttcatac tcagcatact tcttcttccc tattgtaagg
    39301 ttacatacca tactacctaa agccatacct attgcaccta ctagtgcagc agctccccca
    39361 ccacctggta ctgcttcttt ggaagatagt acttctacaa aatcaacaca agttttatct
    39421 gctattttca tgataatcct ccttgaaagg atgtaggaaa atatctcaaa ttgttcgaga
    39481 tattttacga actattttaa gatatctcct actctttatt tataaattaa tttccttaga
    39541 ataatccagc tattactcca ttttcatcaa catctatttt ttctgctgct ggaacttttg
    39601 gtaatcctgg catcttcatt atttctccag taagtgcaac tataaatcca gctcctgctg
    39661 aaattgttgc ttgtcttaca gttattctaa atcctgttgg tctacctaat ttagtttggt
    39721 catcagttaa agaatattga gtcttagcca tacatactgg aactttagta aatccaagtt
    39781 tttctaagtt agctatttcc ttatcagctt caggagtata atctacacca tctgctccat
    39841 aaatcttagt agctatagca tttatcttgt cttttataga taaatcatct tcatagcaga
    39901 atctaaagtt attttcttct tcatccaata atcttaaaac ttctttagct actgcaattc
    39961 caccttctcc accttttgcc catacttcag aaagagcaac attaacacct aattccttac
    40021 acttgtcttc aacaagttta agttcagctt cagtatcaag tgggaatctg ttgattgcaa
    40081 caactgctgg taatccatat acttgagtta tgttttcaac atgttttaat aagtttggaa
    40141 gtcctttttc aagagcttct aggttttcgt tatttaagtc tgctttagca actccaccat
    40201 tgtattttaa tgctcttaca gtagcaacta ttataacagc atctggtttt aagtttgcca
    40261 ttctacattt tatatctaag aatttttctg ctccaaggtc agcaccgaaa ccagcctcag
    40321 ttattacata atctgcaaag tgcatagcca ttcttgtagc tataacagag ttacatccat
    40381 gagctatatt tgcgaatggt ccaccgtgta caaatgatgg tgttccttca agagtttgaa
    40441 ctaggtttgg ttttaaagca tcttttaata atgcagccat agcaccatta gcttttaatt
    40501 gtctagctgt tacaggttca ccttcgtagc tgtatccaac tattatgttt cctaatcttt
    40561 cttttaaatc agatatatca cttgccaaac agaacgctgc cattatttca gaagcaacag
    40621 ttatatcgaa tccatcttgt ctaactgctc catcaccttt tttacccata ccatcaacga
    40681 tatttctaag ttgtctatcg ttcatgtcta cacatcttct ccaagttatt ttcttagggt
    40741 ctattctaag tgcatttcct tggtgaatat ggttatcaag catagcagct aaaagattgt
    40801 tagcagctcc tatagcgtga aaatctcctg tgaagtgtaa gtttatatct tccataggaa
    40861 caacttgtgc atatcctcca ccagctgctc cacctttaac accaaatact ggtcctagag
    40921 atggttctct taaagcaact aatacatttt tgtctagttt agcaaatgca tcagcaacac
    40981 ctatagtagt agtagttttt ccttcacctg ctggagttgg gtttatagct gtagttagta
    41041 ttaacttagc ttttttacca gtctctcttt ttagtagatt gtagtcaact tttgctttgt
    41101 atttaccata aagttctaca tcatcttcac ctagtcctaa tttttttgca acctctctaa
    41161 tatcttgagg cttagcttct tgagctattt caatgtcaga tttaaatccc ataactgaat
    41221 ctcctcccac aacctaaata gatttaaaat ataatttttg taaaaattgt attaatatgt
    41281 aaaaattata ttttataaga taattataca tgtaaaatta cttaaaaact agctactaag
    41341 ctattaaaat attcaaactt aatagctagt tatttatcta attacttact tattacctat
    41401 ctattgccta tgtctaacct ttaaccaact taaaatccca aatcaaaatt tctatctatt
    41461 ttatttatcc ttaagtgaca ttaatttatc ttttattgct ttaatctgtt ctttcgttgt
    41521 ttcactagaa tcatatggag attgattagc tctatcagaa tcaataacat cttggttata
    41581 ataaataaat cctaaagatt ctccatcttc taagttttgg attataaatt cttcatcttc
    41641 cttatttcta attttatttc ctactacaaa tactttattt acacctatat catgtccaag
    41701 tttcttaact tttctataag tctgtagact tctctctcct ggttctacaa ctactataaa
    41761 agcatctaca ccttgggctg ttcctcttcc taagtgttca attcccgctt ccatatccat
    41821 aaccactaca tctttatttt gtagtattaa atgtgagcag agtctcttca aaagaacatg
    41881 ctctggacat acacatccag taccaccaga atctacggtt cccaatgtaa gtaatcttac
    41941 tccattgtat tctttacaga aattttcagg gatatcgtct actttaggat tcatcttaaa
    42001 catttttcca aatgaaccta cagaagcagc tgttctatct gaaactaatt ttttcatttc
    42061 tgatataggt actattgatt catatacttc ttttggaaaa cctagtgcta aagctaaatt
    42121 agcatcaggg tcggcatcaa cagcaacaac tctgtatccg tcttcagcaa acatcctaga
    42181 gagcattgaa gaaaaagtcg ttttacctac tccaccttta cctgttattg ctatcttcat
    42241 aatccctacc accttttatt actatttgct ttttagtagt taattttaca gttaaatatt
    42301 ttttatactc tatattttta tacactaaaa ataaactaca gctagtcctt acaggaacca
    42361 attaaattta ccctactagc ttttcaaagc agcatgtata gttttattat tctttaatat
    42421 tgtttatatt ttatttattc agtatattca ttatattttg atgttcttta ctttaatatt
    42481 tgactttatt caactttatt caaccagaaa cttcttataa aaacgtttat tctataactt
    42541 cactattttt taagcttcag ccttttctgc catcttttct tcaactaatg cttcaaagtg
    42601 aacacgtttt ttctctatat cttctattat tgtagctgct aactcttttg gatttgtatt
    42661 tacagtaaat gtagctccaa ctttatcttt taattcacta gttaatatat ccactgctct
    42721 tgaagaacca gctattggag gcattattcc taaataagtg tctataccag aagctacaac
    42781 atagcatcct atagcaactg ctttttctga catccactct ggagctatac ctacaactgg
    42841 aaggtcacac atatccatat ctaagtaatt agctgctgct ccaactaaat ctagtatacg
    42901 acttatatca acacaagaac ccatatgtag tactggtggg attcctacta atttacagac
    42961 agttgcaaga cctttacctg catatttttc agccgcatca gattccatta aaccaaactt
    43021 agctgctgct gaagctccac aacctgtagt aactactatt atgtcgtttt tgattaattc
    43081 tttcattatt gttacgtgag cctcatttga aactccttta gcattgttac atccaactac
    43141 accagctgct cctcttaata ctccagattt taaacagtct gttaatggct taactgttcc
    43201 tgctgggtct atatgagagt ttacaactct gtctaattga tttactactg cttctacact
    43261 atacccaact gttgcgcctg attttaattc aggtataagt acttttgatt tatctctatt
    43321 tttaaagttt aaaatagctt ctttaactat tgctttagca tcatcaagtg cttgttcttc
    43381 tctaaattca atataagttg aacctgttat cttagctttt ggagaagttg ttacaaattt
    43441 agtatggtaa caatctgcaa ctctagctaa tgcagggaat atacattgaa cgtcaactat
    43501 tacagcttca actgctccag ttattattgc aagttcttgt tgatggaagt ctcctgctat
    43561 ttttacacca tgtctcattg taatctcatt acctgtacaa cacatacctg ctaagtttat
    43621 tccatcagca ccaacttctt tagctaaagc tactaagtct ggctcttctg atgctaaaac
    43681 tatcatttct gataatgctg gctcatgtcc atgtaatatt atgttaactt tattttcttc
    43741 taatactgct aaattcgcct ctgttcttct tggcactgga gttccaaata agatatcgct
    43801 tagacgagta cctatcatag aaccacccca accatcagcc atagaagttc taagactcat
    43861 gtgtattaaa ctatctatat caccagtaca tcctatatga gtagagtgca ttattgttgc
    43921 aatttctcta tctatagctc ttggctctat agcgtattct ttccatattt tttgtcttgg
    43981 ttctggtgca ttttttataa atcttaatgt cccaaaaggt tttccaaatt ccattaatgc
    44041 aatttctgcc acttcatgtg ctacatcata gatatctcta ccttctgtct ctacatccca
    44101 ttctttagct aaagtaatta acttagcttc gtctcttatt ttatagtttc catttggatc
    44161 agctaatgct aatgtatgtg ctatatctct agcatggtct gaatgggcag ctgtcccacc
    44221 agcaaccatt cttgcaaagt ttctacctac tatagtatgc tcatctgctc cacataaccc
    44281 ccttggagtc ttagggctta ttctacatgg tcccattcca catattctac agcaaacacc
    44341 ttgaagacca aaaccacatt gtgccttaaa gccttctttt ctatcataca ttgtttctac
    44401 gccttcttct ctagctttag ctatcatctt ctggctgttt aaatcaattg ttaacatttt
    44461 ttcatccata ttcctatccc ctttttttga tttttaaaat cccccaggtg actgacttca
    44521 tgtcataaaa taataaaata aatgctttct aagttgatta tatactaatt ttcagtgctt
    44581 tgctttagat ttaaatattt ttttaacaaa attagataat acataaaaat tttattttct
    44641 acacttaatt taagttgatt ttatcgcttg tttttggcta tttgttaata tctaaaccta
    44701 cttttttgat acttcagttt aattaaatta tgttttgatt tttttaaatc aaatttgaaa
    44761 tttttctagt catttttata ttattgcaga ttgtttgtgt atttattatc tttcactact
    44821 gtttggactt attacagatt tttactccct aaacttaatt agtaaaaact tcttagaata
    44881 gcattttttg gactaatttt tttgaatttt tttatttttc ctactttaat attaattata
    44941 gcttcaaatt gaacatttgt atatattcat ttgaagctat aattattttt tatataaaac
    45001 ttttttaata cagcattaat ttagacattt tgactatttt ttctacttta attcatttat
    45061 ttcaagcgat ttatcctatt tcatattttt agctacaata gccccatatg caatagaata
    45121 taatttagat tcatgtgaat cagctcttga cactaaaact ataggagctt ttgcacccat
    45181 aattatacct gctgatttag agtctgccat atatgtaaag gctttcccta ttccattacc
    45241 aacctctatt gttggaacca atatgatatc tacatctcca gatacttcac ttttaaaccc
    45301 ttttatctca ctagcttctt ttgaaacagc taaatcaaaa gccagaggac cttctacaat
    45361 tacattttca ccaaattctc cttctttaca agcttttgct aacatatctg catctacagt
    45421 tgcttgcatc ttaggattta ctttctcttt agctgccaaa catgcaactt ttacagtttc
    45481 cattcctaaa gcttttgcag cttttataga attttttaat atctttgcct tttgttcata
    45541 atcaggagct atattcatac ctccatctgt agttataagt aatttatggt atttttctaa
    45601 ctcatatacc ataacatgac ttaataaact atcagttcta agaccatagt ctttatttaa
    45661 cacttctttt aatatgacag atgtatctaa taatcctttc attacaaagt cggcttcttt
    45721 acttgatact aatttaactg ctatttctgc acactctttg atatcatcct tatccactat
    45781 tttgatacca cttaaatcaa atcctatttc ttttgatatt tctcttattt tgccttcatt
    45841 tccaaccaat ataggagtaa ttatatttct ttctacagca tcctttattg ccattaaaac
    45901 ttctttatca tgagctgctg ctacagagag tacaattttc tctcctccct taagttgttc
    45961 taaaatatca tctaattttt ttatcaaaag taacccctcc cttatatata tttaactaaa
    46021 taataccata tttgctatac cttttctcaa tttatttttt atgttataaa gaatttatat
    46081 aactaaaaga cagtttaata ttttattctg ggatttcatc aataactctt aaaagctttg
    46141 caggaacacc acctacaata gtattttctg caacattttt tgttacaact gctccagcag
    46201 cgataattgc attatctcca atagtaactc ctggaactac agttacatta gcaccaatcc
    46261 atacattttt tcctattgtg attggagaag gataagttgt accacgtttc tctggtgcaa
    46321 taccatggtt aagtgtagcc aataccacat tatgcccaat ttgcgtatta tctccaatct
    46381 caatcccccc ctggtcttga aaatggcaac tgctatttat aaaaacattt tttcctattg
    46441 tgatgttttt tccacaatct gtataaaatg gtggaaacat aaaaaatgat tcatctacct
    46501 tttttccaat taactcagaa aataaactac ggacttcttc tggttcatga taagaagaat
    46561 ttaacttact tgtgattttc attgcttcat tactcaactt aaccataaat tcatgtattt
    46621 ctgatttact agaaacaggt tttctttgat tgatatgtgt taaaaattca ttgatattca
    46681 ttttatacat cctttcaata tgtgctttaa tttatttgta cattctaaat aatattttaa
    46741 taaaaattaa tttaatttat ttttattaaa atatcattgc ttcttttagt gtaatataca
    46801 attatatcta tatcaaatat ctatagtaaa tacttaacta tacttaaaaa gcatataaat
    46861 tgatataatt atataataga taagttaata ttgaggaggg ataattatgg aaataagagt
    46921 attaagatat tttttagcaa ttgcaagaga agaaagtata tctggtgcgg caaccttttt
    46981 acatataaca caaccaacac tttctagaca aattatggaa cttgaagaag agttgggagt
    47041 taaattattg gttcgaggaa atagaaaaat caccttaaca aatgaaggta ggcttttaca
    47101 taaaagagca gaagaaatta tatcattagt tgataaggtt gaattagaat ttgacaatcc
    47161 caatgagatt attagtggtg atatatatat aggaagtggt gaaactgaag ctatgcgact
    47221 tatagctaga actgcccatc attttcatca aaaatacccc aatgtaagat atcacctttt
    47281 tagtgggaat gctgatgatg tgactgaaaa attagacaag ggactgcttg attttggtat
    47341 tttaattgaa cctgcaaata tacagaagta tgattatgca caaattcctg ctactgatgt
    47401 atggggttta ctaatgcgta aagatagtgc ccttgcatca caaagcacaa taaatccaaa
    47461 agatttatgc aatatacctc ttattatttc aagacagaca cttgttgata aagcaatttc
    47521 taaatggctt caagacagta atgaaaaact aaacattatt gcaacataca atttagttta
    47581 taacgcttct cttttggtag aagaaggtct gggctacgct ctgtgtttag atagactcgt
    47641 caatactaca gaaaacagta atctttgttt tagacctcta tctcctcctt tagaagctaa
    47701 attaaatatt atatggaaaa aacatcaaat attttcaaag gcagcagaaa aatttttact
    47761 tcaaatacaa aaagattttt cttctatcat gttggataca tctaagttgg atacacctaa
    47821 agaaaaataa atcataataa aaggtgagag tgtctcaaaa tgaactttgc actgtataag
    47881 gcactctttt ttatacaaag taaaatatat gttcatcatt tcagcactaa aaaagaggct
    47941 tatctctatt ttgagatagc cccacttaac caataaaatt aaatacaact taacaatatc
    48001 cttacttatt tattctaagt tctcttagac aagcaatagt aatttcatct tgtggattta
    48061 attcataagc tctttctata tagtaagttg catcatcaat atcaccatta tttaaataag
    48121 ccattcctaa attgcaaagt atttctgggt cttcctttat cttagccgct tgttcaaaat
    48181 actctattgc tttatctaaa ttattcttat aagcctcaca aagccccaat tccactatag
    48241 tatccacttg attaggcttt attatcaata tcttctcaag atacatctta gcctgctcta
    48301 tttcgcccat acccttatat ccaagtgcta tcataaacag caagttccac caatccatat
    48361 gttcttcttc taatggaagt aacttctcta atccttcctt aaacttacct tggaatacaa
    48421 gtgtatatcc ttcttcatat tgaactttaa agtccatctt gcccagattc tcttgtacct
    48481 cagctactaa atcagcatct aaccctagcc ttaacgcttc ttcccaagtc agcttagtct
    48541 ttaaatattg accttgatta taataatggt agcctaagtg ataataacct aatgcaaaat
    48601 tctcatcaac atcaattacc ttttcaagct tagccatggc ttcaagtaag aatgcattca
    48661 ttgccttatc atccatatct ttttgatact gtttagctat ctcttggcaa acaatcgcat
    48721 agttatacat tgcatctaaa tcatctggat acattgttat aaatgctttt atatatatta
    48781 aagaatctgt atgttcacca atctcaaagt attttctcga catatatccc atatacgatt
    48841 tcaaatccaa atcaatatct tttgataaag catttaaaaa cttctcatac tctgcattgt
    48901 ttttaaactt actatctatt ccaattatat atatcattgc atctgctata gacattgaat
    48961 ttaacttatc ttgagcagtc ttttctttta tcccttttac taatacctca ttttttatag
    49021 gtacatctaa tccacctttt ggcacttcat agctttttaa tttaaactct ccaccatgtt
    49081 ttatagttat aaaagctaat tcttctgctt tttttagcaa atatttttct atcttaaaat
    49141 ccattttatc ctcctaaata cttaaatctc tactttatga gttttagatt tctcaaatat
    49201 ttttttatca tcatactcaa ataagactac attttctact tctcttattt ctgtatttat
    49261 atcatttttt aaatccacaa cattaaatct aaatggctca aaatgaacat attttattat
    49321 atgtttagct ggtttatcaa caaaactagg taaatatgtt gataagttat cattatcttg
    49381 taaaaataca tggcatctat tcttaaaatc atccacatct agcttattaa aaaactgtgg
    49441 tatatttgat gttttaccca gtgatatata gtcatatttt aatatatcgt tgaatacatc
    49501 attatctatg ttgatttttt cattatagaa atccatcaat attttatata actgattctt
    49561 tccttgtgcc aaatcaaagt agccattttc atcaaaatac ttggcaaaaa cctcaaagaa
    49621 tttaaaaggg ctttttttat aaaatctgcc tataatatat ctcatagata acacaaagtt
    49681 tttagaattg tagtatctct ccagtatatc ttctatatcc tttagcttca aagtttcttc
    49741 atagcttata taattattgt ataaaaactc atatggagca taatccttat atttaaaacc
    49801 atgctcatct gcaacttttc tcatacctgt ccctttaatc atctttaaaa agcctaattg
    49861 caaatgctct atacctagat taaatacatc attaaaagaa ttttcaaagc tcttataatc
    49921 ttcataaggc agtcctgcaa ttaagtctaa gtgctgatgt atatttctat aagatgctac
    49981 agtttgtaca acatgagaca actttttaaa atcatccctt ctacctactg catcaagtgt
    50041 tttttcattc gttgactgaa cacctatttc aaattgaaaa agaccttctt tacaatcagc
    50101 caaaaactca agcatcttat catctatcaa atacgctgta acttcaaaat ggtaagttgt
    50161 atatccatta tcatttttca taaggaaatc catgatttcc atagccactt ttctatttgc
    50221 attgaatgtc ctatctataa actttatctg tgatactctt gcatctatta aagcttttaa
    50281 atcactcttt atcctgtcta aactaaaata ccttagacct ggaatagtag atgataaaca
    50341 gtattggcag ttaaaagggc aacctcttga tgattcataa tacactattc tattttcata
    50401 ttcttttgga ttcaagtttt cataaggact aggaatctca tccaaatctt gaagtaattc
    50461 cataggctta ttcactatta tctcattgcc ttgtctatat acaagtccat ttacatcatt
    50521 tatttccatc tttccttgca agtgaagtac taagtctcta aaaattaatt ctccctctcc
    50581 ataaagtata taatccacaa aatcatactt tttcattgcc tcataactat cataactcac
    50641 ttccggtccg cccaaagcta ttttagtatt aggtctaatt ttttttagat tatcacaaag
    50701 tttaactata tctcccacat tccagatata tgttgagaat aatattatat catattcatt
    50761 tttatatata tcctttaaaa tatagtccaa atcgttattt atcgtgtatt ctttcatatt
    50821 aacttctatc aaatctctta caaattcttt tagatatctt atagccaaat ttgtatgaat
    50881 aaacttggaa tttaatgttg ttaataaaat tttcaaatat ttctcacctc tttttaagat
    50941 tatactgtct ttttgtaccc cttataggtt aaaatagtat atgtaaagtc taaataatat
    51001 ctatataatt aatttattaa tttcaagtaa tgtgaggaaa ggaagttttt tatggaatta
    51061 attgaaaaag tatctatcaa ctctttatca aaaagagatt tactacttat tataaagtct
    51121 cttgaataca ctaatgaaaa tactcaaatt agtgatttca ttgaccttag gaataatata
    51181 gtaaaggaat tatgtttttt aactgacact aaggaaaaag attttttaga ttatctagaa
    51241 aaaaatagca cactctaata acctaaacct ttttaaactt taatatataa atacctgatt
    51301 tagaatttat ttttatgctt aatatattat cattaaaggt tttcgacata actgttattt
    51361 gattaaatat agggtctatt atagtattac cttcttcaag tcttataaga cttcttgggt
    51421 ctatgctttt attatatgcc ttaactccaa ttttatattt tctcaattta acaacatatt
    51481 cggacataga aaaaataaaa tctatattat cttcatttaa tttactatct tcacccaaaa
    51541 tggataatat agattttttt atgctattaa ttccttcatt ttctacagtt ctatcttcca
    51601 tatatacatc atatagaaca tttaaaacat ttaataaaat actttcttca tcataatctt
    51661 cagatttcaa atcaatgtac ttatcaatta attgtattgc tttaatcctt gccatctgaa
    51721 actttacaac atctgatttg tctaattcta tagaaaatcc tattatcttg tctttaatac
    51781 tctgtagtaa ttcgacatac tctattttac tattatttat aagattgtgc ataatggaat
    51841 tatacaccac agaacctaat aaataatcaa ataaaagttc ttctttttta aaataaataa
    51901 catacttttt tattagttta ataacctcat cttcactttt ttgaatatta gtatttgata
    51961 aaattagtgg taataaaata tttaaaaaat tgatatttcc tattttttct ccataaaatc
    52021 tatagtaaat aagttcatct ctcagtctat tttcgtctat atacacacat ctttgcgttt
    52081 tatcatctat taaggatttt gtatttatta tagttaaatc tttaagagcc ctacttgcac
    52141 tactaaaaga agatgtgtag acttttttta gtaattcgtc attcataaat atatcctttc
    52201 tgaaaaattg aatgtaaata aattgttatt aaaaaaaaag agacagcccg tttaagaagc
    52261 tgtcatatat agggggatag tagtgtacaa aaatcattgt aatctactat ctatatatta
    52321 ttatacgata ctactttttt atatgtaaag catcagtttt cgacatccct atattttaca
    52381 gttatacaaa ttgcgtacta agttcaattt tattcttttt atttcaacat tttatcatgc
    52441 tacattcttt caggagcgtg cattcccaac aatttcagag aattttctaa agtttgtttt
    52501 gttgctaaaa ctaatgcaag tcttgctttt cttatttcca aattttctac taaaattgga
    52561 ttgtcatgat aaaatttatt aaacgcttga gctaaatcta gcataaatct agtcactata
    52621 tgtggctcat ttttctttag agcaagtaaa atattcttat tgaatgattc tataactcta
    52681 agaacttctg cactatcttc atcagctaat agactataat ctatatcaga agtcacttct
    52741 acttctgctt ttcttaaaac tgcacagcat ctagcatgtg tatactgaac atatggtcca
    52801 gtctcaccat caaaactaag cgttctttcc caagagaagg tatagtcttt aattctacta
    52861 ttagataact cttggaatac tactgcccca acacctactt gcttagatat ttcatccaca
    52921 tttttagcat ttgggttttt agctagtata atttccttag ttttatctat agcttgattt
    52981 aaagcatctt ctaaaaatac tactcttcct tttctagtcg acatagtacc ttcttcaagt
    53041 gcaaccatac caaatccaac atgaattaaa tcttttgccc aatcatatcc cataagctca
    53101 attaccttaa accattgttg gaaatgtaag ttttgttgag aaccaactac atatatacac
    53161 ttgtcaaaat cataagtttc ttttctatat attgcagcag ctaaatctct tgtcatatat
    53221 aacgttgagc catcattctt agtaataagt gctggtggca ttttatactc ttctaaatca
    53281 acaattcttg ctcctttaga ctcttttaaa agatttttct cttctaatag ttcgataact
    53341 ctgttcattt tatcagaata aaaactctct cctgctaaag aatcaaattc tatatctaaa
    53401 agtttataca ctctgttaaa ctcttttaaa ctctcatttc taaaccattg ccataattct
    53461 tgtgcttctt cgtcaccatt ttctaattta ttaaaccatg ctcttgcttc atcttccatt
    53521 tctgggtgtt gttcagcttc atcatggaat cttacgtata attttagtaa ttctggaatt
    53581 ggattagact ctactacttc tttttctccc cactttttaa aggccactat taacttacca
    53641 aactgagttc catagtcacc taaatgattt attcttatag tcttgtatcc ttgcgaatca
    53701 tatatcttat ataaagcatt acctataact gtagttctta tgtgccctat atggaaaggt
    53761 tttgctatat ttggagaaga atattcaact attacagttt tatcttttcc aaaatcacta
    53821 tgtccgtaat tttctttttc atctaaaact tttttaatta cagtttctgc taattgagat
    53881 ttatttacaa agaaatttac atatccgcct aattgtatta cttttgttat ctctccactt
    53941 ggttctatac tttccgccaa ttcacttgct atcatgtttg gagcttttct aaacactttg
    54001 gctaatttaa agcatggaaa agcatagtcc cccatatcct tatttggtgg aacttctata
    54061 agtgcctcta tctcttcttt cgataaatct tctatttttt ctttaaggca atttgatatt
    54121 gccaccttaa aatcttgcat tctgttgctc ctctctacat tgttcctggt ttatattgtg
    54181 cttatctctt tatgttaaat caaatttcgt gttttttcaa gtatttcatt acagtgtaaa
    54241 acactttagt aattatgttt tctgtatata ttaaaaatac tataattgca gtttttaatc
    54301 acactattat tgatttttta taagactttt ttctatttta ttgtttcttg tactatccag
    54361 ttgactttat atactttttt taatctttta tctattttca tacgtcaatg ctaattttat
    54421 atacttttct tttaatcttt tattaatttt tatacatcaa tgttaacttt atataagttt
    54481 ttttaatctt ttatctgttt tcatacatca atgtcagctt tatatacttt ttttactttt
    54541 ttatctattt ttatacatca atattagctt tatatacttt ttttaatctt ttatctattt
    54601 tctataaatc tttttcactc ttaactttta ttccttcttc ttctaatagc cttgctgtta
    54661 tacctttccc tggtatcttt ttacctgaaa aactaccatc atatattaga ttacatccac
    54721 aagaggggct accctctttt agtattgctt cgctacatac atataaattt gctattttta
    54781 gagtttcatt agcccctctt acaaagtttt ctgttacatc cttattatct ttagtaatta
    54841 cttttccatc aactatctca gcaggtggtc ttggtgtaga tagaccacct aactgctctg
    54901 ggcatacaag tgtaaattct ttgcctttta aatattcctt gactttttca ttatcattat
    54961 tatcgccact atatttacaa tttattccta ttaaacatgc agataccaat atcataaaaa
    55021 tgtgctccta tacaaattct taatatattc tcatacaaat tactaatgta ttcttataca
    55081 aattatttta atttaacaat tgttactcct gctccacctt ctccatattg accacctctt
    55141 tgtgatttta catgtctatt tcttcttaaa atgtcttgta gccctgcttt taatactcct
    55201 gtacctattc catgtattac tgttacagac tctaaacctg ccacatatgc atcatcaaga
    55261 tacttttcta cttccattat agcttcttct aggtttaacc ctctaaggtc aacttcattt
    55321 tttacactac cagattttga ccttataatg tttctcgttg atttagttac atttgttgaa
    55381 acatctttgc gtgttttttg taaagactta aaaggtagag tcatcttcat tataccaatt
    55441 tgcactactg cttcttttct ctttttatct actgaaacta cactaccatt ttgatttaaa
    55501 gttattacct taacttcttc acctggcttt aagtctttta tctccttatt tgataccttt
    55561 ggaactatca tactttttac tgttggctga agacttccca tagagctggt caattctttt
    55621 ctaagttctt ctatcttacg attcttttct tttgaagctc tttcttgttc aagacttcta
    55681 agttctttta ttataatatc tacttcttct ttagcttgtc ttataattga aaatgcttct
    55741 gatttagctt tttcaatcat cttatctctt tgactgacca atttttcaag cttttcgtca
    55801 tactctactt tcagtttttc aatctcttct tttaatcttt cagcttcttc tctatcttct
    55861 actgctttga ttctattctt ttcaacattc tgtaacacat cttctaaagc tatgttttct
    55921 gtatttatat attctttagc acgtgaaatc acataatcac ttagcccaag ttttctagaa
    55981 atctcaaacg catttgattt tccgggaaca cctataagta acttatatgt tgggcttaaa
    56041 gtttctatat caaattcaac tgctgcattt tctacacctg gtttagtaag agcataattt
    56101 ttaagctcac tatagtgagt ggtagctata catttagctc ctacagaatt tatatcttct
    56161 aatactgcta ttgcaagggc agctccttca acagggtctg ttcccgctcc taactcgtca
    56221 aatataacta aagagtctgc tgttacgttt tgtaatatag atactatatt cgtcatatga
    56281 gatgaaaatg ttgatagact ctgttcaata ctctgctcat ctcctatatc tgcaaataca
    56341 ttatcatata cacacatact acttccataa tcagcaggta tatgaagtcc actttgtgtc
    56401 ataagtgcaa atagacctac agtttttatt gtaacagtct taccacctgt gtttggacct
    56461 gttatcacca atgtatggaa gtctcttcct aaataaattg tatttgctac taccttcttt
    56521 ttatccagca gtggatgtct tccatttttt atgtttaaat atttatcctc atttaaagtt
    56581 ggttctattc ctctcatctg tatagaaagt tttccctttg aaaatgcaaa gtccaatctt
    56641 ccaagtatct cttgattaga aattaagtct tcactaactt ctccaaccat agcagaaagc
    56701 tctgataaaa ttctttctat ttcttcttgc tcacctagtt ttaactgtct caattcattg
    56761 ttcatctcta ctatagtcat tggctctata aaaagtgtcg caccagaaga tgactggtca
    56821 tgcactatac cagctacttg cgacctatat tcagatttta caggtactac aaatctatca
    56881 cctcttagag aaattattgc atcttgtaag tacttttgat aagtagttga acttattatt
    56941 gaatttaatt ttgacctaat ggattgattt ttttgggcta ttcttcttct gatatctcta
    57001 agtgtacttg aagcattgtc agatatttct acttcactaa ttatagcatt gtatatctgg
    57061 tcttctatgt ccttgtatac atatagagaa tttgatagag attgtattat tggataatta
    57121 aaatcttctt catcactact agataagcta tttcttaatc ttctagccac tcttaaggta
    57181 tctgctatca taatcagact tcctgggtca agagaagcac ctatataagc tctctttacc
    57241 ttatcttcta tatcatgaat accttccaac cctactgaac ctctctttat aataattgat
    57301 tgagcttcac tagtttcttc tagcatagat ttcacttcta caaaatcagt atttggtact
    57361 aaattttcta tatattttag acctaaagaa gatgaggctt tcttctttaa caaatctatt
    57421 attttattgt attctagaac tcttaacgat ttctcattca ttttctatat tcaccatcct
    57481 cgatatttta acttttttat ttaattttaa aaatgtatgt tttcaagcat ttccaataaa
    57541 aactgcaagt tttatttctt tttcatttct aaaattatta tattttttct cttttagata
    57601 tattttacac atattttgta ttatactatg tatgtagcta tctatatatt atacataaac
    57661 atgctttatt tgtaatataa tctgttaaaa aatcttattt aatattttta aaggagttta
    57721 ttttagctta taaaaaaaaa tagctactta tatagataaa ttaaggagga acatgagtca
    57781 tgggaaacgt attagaagga ttaaaaccag aaagtgtttt taaaaacttt gagaaaatat
    57841 ctcaaattcc tagaggttct ggaaatgaaa aaggcataag tgatttttta cttagctttg
    57901 gtaaaaattt aggtcttgaa actattcagg atgaaagctt aaacataata ataagaaagc
    57961 ctgctacaaa aggatatgaa aattgtccag gtgtagttct tcaaggacat atggatatgg
    58021 tttgcgaaaa agaaaagaat gttgaacatg attttcttaa agaccctata aaattaagaa
    58081 ttgatggtga catgatatat gctactggaa ctactctagg agctgacaat ggtatagctg
    58141 ttgctatggg tatggctata ttagaagata atactttaga gcatcctgca ctagaagttt
    58201 tagttactgt taatgaagaa gatggtatga atggtgcaga tgcactagac cctagcttaa
    58261 ttaaaggtca atatatccta aatatggact ccgaggagga aggatactta ttagttagct
    58321 gtgctggtgg taaaacttgt gttgttagtt tacctgttga atacaaggaa gttaaaggtg
    58381 acaaacaagg attattagtt gaggttactg gactattagg tggacactct ggtatggaaa
    58441 ttgttttaca aagagcaaat gctaacaaag caatagctag agtattaagt gtattgaatg
    58501 ttgattatga attagcttct gttgatggtg gtactaagca caatgctata cctcgtgaag
    58561 ctaaatgtgt aatagctgtt aataaagctg atgttgaatc tgctaaaaaa caaataaatg
    58621 acattttaac tgcatttaaa catgagttta ctacttctga ccctggcatg acttactcag
    58681 tagctgaaac ttctgtagac aaagttctta ctaaagattg taaagaaaaa gttgttcaaa
    58741 tgtcttgctt aactcctcat ggagttcaat cagtaagtct tgatatagaa ggattagttg
    58801 aaagttctac taacttcgct ataatcgaaa ctaaagaatc tactattgaa tttttaactt
    58861 ctgttagaag ttctgttatg tctattagag atgaaatcgc tgatagaata agacttttgg
    58921 ctcaagcttt aggtgctaat tacgacctta tagctcaata cccagcttgg gaatttaaaa
    58981 aaggctcaaa attagaaaaa atatgcagtg aaacttatga gaaattaact ggtaaagttc
    59041 ctactgttat ggctctacat gctggtcttg agtgtggatt attacttgat aaacttcctc
    59101 atgctgaagc tatctctata ggaccagata tgtttgatgt tcatactcca aatgaacatg
    59161 ttagtatccc ttctgtagct aatgtttggg attatgttat tgaaatatta aaatctatga
    59221 accaatatta aatttaagtt ttgattctaa gtagatttta attttaagta atctatataa
    59281 aattaactct gtaaattaat actactgtta gtataatatt tactttcaat attaacttat
    59341 agaaataaag cctaatgata gataataaca tgtatcatta ggctttattg taatttaaat
    59401 attattaagc tttattctgt tttaaaatta attcttatac tattttaaat ctatttcttc
    59461 cttgtctctt agaatcataa agagcattat ctgctatttg atacagttca tcatactctt
    59521 gaccaggctt tgctataact acccccatac taatagataa actttctcca tttggcaact
    59581 taatgagatg aatatgattg tatacatcat tacatttatt aataagttct tccttattta
    59641 ttatatcatt gatataaaca ctaaattcat ctccacccaa acgacctact atatcatttt
    59701 ttgcaaaact ttcatcaagt acctttgcaa catcaataag tactttatct cctaacaagt
    59761 gaccataagt atcattaatc gacttaaaat aatcaatatc tataatcaat aaagcaccat
    59821 ttttttcagt tgataaattc aacaaatttt gtgcagttaa ttttctaata tatgctgcat
    59881 tataaatatt ggttaaacta tccctttgag ccttatctaa caatctattc ttttcctctt
    59941 tttcagaatc aatattacat attttcccga ttacatacat tggtattttt ctatcattat
    60001 aaattatttt tgaagtaact ctaatccaat gaaaagtatc atcaagcata atagaataca
    60061 tttcagttgt tccattttct tgtgatttta ttacatcaaa aaacttttta ttatattctt
    60121 tccttatttt atctctttca ttttctatat tgccattttc actttcctta tcatttttat
    60181 ccaaaccata ataagtctca ataccctttt ctggcataga aattatcaat ttatcttgct
    60241 tataatcata ctcaaaaaag tattcacttg tcatttcata caactgagta tgttttttga
    60301 gttctaaaga tatcttctcg ttcatacttg tacgagtatg caaaccaaat gccataaatc
    60361 caataataca aattaataaa ctggctacaa taaaaatagt ctcaattgga ttctgttgca
    60421 taatataaag caacgagaag tcttgtctat atgtagtatt ttggtagaca attgattgca
    60481 ttttttctga tgaaatactt ataatagctt tattaaatat attcaataat tcttgtttat
    60541 cttttttaat tataccaaaa caaatactct ttgctttata agtttgagga actagacgca
    60601 catttttata cttaggttga ttcatatagt actgcataga ataaccttcg ccataagtgt
    60661 aatctgcata gcctttatta acagcttcta tacactcttc taaattatca taccaaacta
    60721 tattctcttc aaactgacca taataattaa tacctttggc tagagctaca cgttttccat
    60781 ctaaactatt ctcatttaat ttattattaa tcatcatagt atattgagta gaaagaaaag
    60841 gccgagacat aactatatta tgttcacgag agtattcata atcataaatc attcctgcta
    60901 caatatcaat ttcacgcttt ttagtcatgt tttctaaatc ttcttgagat tttacaggca
    60961 caagttcaaa ttctaagcct gttttatcac atatatgatt taacaaatca attgatatac
    61021 ctcttaattc acctgtatct ttatctatat tttgaaatgg tggtttgttt aaaagtactc
    61081 ctacttttaa tttttctgtc tctttaatgt aatcagtttc ttcatcagat aaaaacattt
    61141 gattgttctc tggtgtaaag tatttttcat ataaagtagt tgaaaaatat gggtctttct
    61201 gttcaatatt attaattgct gagttaagct tttgagttaa atttgtacta cctttaggag
    61261 taatgaaata aaatggtttt gatgaaaatt ttgctacagt acgtacacct tctagtgggt
    61321 tcatactaac attaagaagt acatctgctt taccactctt tagtgcttct agttgctcat
    61381 catttgtttt acaatatacc aattcaggtg acataagatt cattttacaa tactcatcta
    61441 actctttaag tctagtatca gaattttcta ttacagcaat acggattttt ttcacatctt
    61501 gtgaactcat tatgttatca atatctgtat tttcatataa tgcttgtagt actgtatttg
    61561 ctactccata actatttcct gcataattat atattttttg cattggctca ttgtataaca
    61621 tacctcccat tatatcaatt tcaccatttt ctaacatctt cattaatttt gtaatagatt
    61681 catttatctc tccaggaact tgaacaaatt cataattcca tccagtatac tgtgcaattt
    61741 ctagtaaata ttcatatgta tacccaaaat agttaccttg ctcatctatt tcagttaaac
    61801 ctttttgaat aggataagca acacgaatgg tacgattatc attacctata gcgttgatac
    61861 caaaagaaaa tatgcaagaa gtgaatatag atgtaattac aataattaat aaaagtaatt
    61921 tttttaaatt cactacacaa tcctccatat agttaagatt tacagtataa attactgcat
    61981 aaacttgtag acatgtaaaa atagattagt atataaaagg atatataaat ctacttagaa
    62041 atttaaatat ccttaatata tacaatatat ttaaattata actcatttat atgtctatta
    62101 caacaaattt accctgctaa atacctactc tatatcccat aattttacaa gttgtagtga
    62161 agtttaagtt ctatctcacc tcaaatttag cagtgtttct atttacagtt cttttatatt
    62221 tcacatctgg ataacccaat ataaatgaag tgataacagc ttcgttctcg ctcattccta
    62281 acagttcttt tatctttggc tctacatttg atgccataac aaaaaatcca ttataacaaa
    62341 ttcctaatcc ttgagaatat gccataagtt ccatattaga tgcagccagt cctccatcta
    62401 cataagcact tccacctaga gacatatctc ctacaactac cataagtagt ggggcatcat
    62461 aaaataaaga atcatttccg ttctctttat atcttttgta cattttctta aatgtatttt
    62521 tatagataga acgtacaggg tcattgtcat cagtatctaa tgccaaatta taaagacctt
    62581 gtattgccag ttctttcact tcttttagct tttcttttac taatatgtat cttataggct
    62641 gtctattacc acctgttggt gtgtatctac cagcttccac tatgtttttt attttttctt
    62701 cttctacctc tatatcttta tattgtctta tacttcttct aaatttaata aaattcaata
    62761 acttttcggg ttctaattca aaactttcac gattgtattc tattacttca tttttatcaa
    62821 atccttgcat ttctattgca tctgttgggc aaattgccat acagtgacca cagttaatac
    62881 agagaatatt tttaaccttt gctttatcat ccttaacttt tattgcattt acaagacagt
    62941 ctgattcaca catcccacag cctacacatt tttctaaatt tatgtctatc attccaaatt
    63001 attcctcctt ttaaaaaatt tactttagta taaaacaaat agttgtctaa gtcaattttt
    63061 atttttttaa caatatacta gccttaacta cactttaatg gagaaaaata tacataaatt
    63121 ggaacttaaa ttagaactta ttcaaagtaa aagataaaag aagtagatgt ctaaaagtga
    63181 actttttagt taattagata ttctttattg tatgaaatca agtggatatt tatcatttca
    63241 acaataaaaa aggcttacct ctattttgga tatgtccctg tcaagtagac agactaaaaa
    63301 aaggctatct ttatgcactg agatagcccc ttttcccaac tactattgtt tatcttaata
    63361 ttagtcaata aatttaattt gctataacct gtaagttaca ttttaatttg atgtaacttt
    63421 aaattacatt tttaagtaat atctttaaat tatattctta aaatatatca ttaaactaca
    63481 ttttaattaa tctaaaaact atatcttaat taatctaaga aacttacatt ataatctttt
    63541 agtttcccat tatcatatac aatctttgca ctaaagatat ttccagtctt agcccaattt
    63601 aagaacagcc tgtcaccttc ccacatattt aaatcgaata ttttatcatt atcaacccaa
    63661 attaaatttc cttctttaca atcatttaaa tctttttcag ataattcacc ttcaaactca
    63721 tctgagaaat acaaaaacat ataccaatct tcttcatctt taaattttgg aaaagttatc
    63781 aaccctttat atgacaatga tttaagagta agaccagtct cttcttttac ttctctaatt
    63841 acacattctt ctggagtctc tccttgctca aactttcctc caactcctac atacttacct
    63901 tcatgtatat catctttctt tttatttctg tacaacatca aagtcttgtc atctttttct
    63961 atataacaaa tagttgtaag tttcatgtat ctcgccttgc tttctcttta tattctcaac
    64021 taattatttt cagcactttc aagcacatca cttatattct tcattgcatt aagagaaagc
    64081 tctgcaaatt catcaaatgg aatccctata tcttctatac ttctcattgc atctctattt
    64141 acatttgaag caaatctcgt ttctttcatt cttttaataa cagacttagt ctttacactg
    64201 gtaagttttt tatctggata aactaaagct atagcagtta taaacccagt tatagggtct
    64261 gctgcatata ttgctttttc cattaggcta gttcttgtaa ctcccattcc atcatgatga
    64321 ccaagtattg ctctatacat ttcttcgtca ccgaagtttt cttttttaag cagttctact
    64381 gttgttgtag catgtccctc acatatttct cctggctttc ttccaactat atctgaatcc
    64441 aaatcatgta aaagacctgc cactgcccac tcttcttctt tttctggctc tagtcttttg
    64501 gctaattctc tcataacagc ttcaacagca taacaatgtt ttctcattct atcagtttct
    64561 aaatacttaa ataaaatttc taaagcttct tctctcattt tcaccaatcc ccctgttttt
    64621 tcacatatat gatattttta tatattaata cacataaatt caagttttta tatatattca
    64681 tgtatatttt tcatgttata tctattctac acctttatag tagtgtcctg caactgttcc
    64741 aatttcatct aatttcttat acaagtctgt agcccttttt ccaagtttat aattattact
    64801 caatacctca atgtgagctt caataacctt tttaaccatc tcactatctt caactgtaaa
    64861 atctatccta aatacatcta ttccaatttc ctctaactca tatagattgt ccaacataca
    64921 agtaaccttc gaattgtaaa ttgtacttct acagaatata tcttgtgtta tcctataact
    64981 tgcatccgta aagtctctta aagcataact actttcctta catttagcaa ctctcttgtc
    65041 ctttttacaa tccctaacaa ctactcccat aggacagtat tctgtaacca tcataggagc
    65101 atatccatat attactgctt caatttctac atctgtatat tttaaagttt gtttaatttc
    65161 atttaagttt agttcttgtg acaaacaaac tgtacttgct ccctcttctt tgaagtggtt
    65221 tatactttca ctattgaatg catttaagta gcaatctata tttaagtctt ttttcttaaa
    65281 gtattccata gagccccagt taccaatttg aaccttatta attcctttta tattatccaa
    65341 tatctcaaat tgcttgtact ctttatttct aacaatcctt ggtgctgaat agattaattt
    65401 tttgtcagtg actgctgtat tagagtggtt cttattcaaa gtatctattt tacttgtatt
    65461 tgacaaagta tatatgttat ttgtatttga acccattagt tctttaggaa tacctaaaga
    65521 tgaagaagta tataaatcca tagaaatact tgaatctata gaaatacttg agaccatttg
    65581 taaagctttt gataatgtat ttatatcttc ataatagatt aaatctacat tataatttaa
    65641 tactgcttct aattgctcca agttcttaac tttaactcta atcttaggaa ctgtatcaag
    65701 ttttttctgt ttatttactc tatttggctg gtattttaat ctattcttct taaattttct
    65761 atcttttatc tttattcttt cttcatctaa aagctcaata caatcccttc tcatctgatt
    65821 taacacactt ataggcatac ttacaccttc atccaggtca atttctatct cttttaactt
    65881 atatggtgta tttccaagct tttgaatttg tgtttctatt ttttctttac ttaaagccac
    65941 ctttatagct ttttcaacta ttttgtctcc ttctaccacc gatttatttc cacaaaaatc
    66001 acttataaaa agctctggct ttttacctag tttaatactc acttttgcac gtatattagt
    66061 ttttatgaac tctttatctt gctgaaatgt agcttgtact ctatccatca attcgctgtc
    66121 agaagtctta tatataagct gatttctctt tgcttctcca acaaaatcta attctatcaa
    66181 atccccttta tgtgctattg tagttatctc tccatccttt attattcttc caatagtacc
    66241 tccacctaag ttaattccat cacctttctt aagcgtattt tctagcatta ctctaagtct
    66301 tttgttcttt ctattataat ctaaaacttt tccaatatat agcccttgat tattaggtct
    66361 attagagttc attatatcct taccaacttc acctaatata tatccttttg taaactttct
    66421 attaaatatg gtatacaaat tatttattat ttcctcagaa atacttattt ttttcttatc
    66481 tacaaattca ttaatcgctt ctctatatcc tgaaaccaca gtagctacat actctggctt
    66541 tttcattcta ccttcaattt ttaaagataa tacacctgct tcaattattt tatccaaatc
    66601 ttctatagta cttaaatctc taggactcaa caaataatct ccattacttt ttactacttc
    66661 tcctgtattt atatctatca actcatactt ttgtctacat ggctgtgcac acctacctct
    66721 atttcccgac ctattgccta tcatactact cataaggcat tgacccgagt aacatacaca
    66781 taaagctcca tgtacaaaaa cttctatatc tacagttgta ttttcacaaa tccattttat
    66841 ctcatcaaca tttaattctc ttgctaaaac aactctatca aaccctacac tctgaaggta
    66901 ttttacatct tctaaggaat gtgctaccat ttgagtgctt gcatgtagtt caaagtcagg
    66961 taatagtttt tttataagtc ttgccattcc tatatcctgt actattaaag catcaatatc
    67021 tatatcgtat aaaaatttta catactctac aaaatcttct atttcatact gttttatcaa
    67081 tgtatttaca gtaacaaaga cttgcactcc ccttatatga gagtatttaa cagcctcaat
    67141 cagctcattt ctatcaaagt tatttgcaga tgctctagca ctaaagtctt taccacctaa
    67201 atacacagca tttgctccgt tttgaactgc tgcttttaat gaatcaaaag agcctactgg
    67261 tgcaagtaat tctatttttt tcatattttt ctcctctatt tatacttgtt aggttaatat
    67321 aattatatct ataacttaaa tatttgtatt aaatttatat atttcttact atattttaaa
    67381 ttcttcaatc aatttatata ttagttattt atatttggtt catttgagtt aacatataca
    67441 ttatttacta tatttagtat atttctatta acttatatat tgattactgt attttaaatt
    67501 cttcaattaa tttatatact gcttactaaa tttaaaacat tttaattaat ttgtacactg
    67561 tttactatac ttgaaatact tctattaatc tatatattgc ctactatact taagacattt
    67621 taattaattt atacactgtt tactatactt gaaatacttc tattaatcta tatattgcct
    67681 actatactta agacatttta attaatttat acactgctta ctatacttaa aatacttcta
    67741 ttaacttata cattgcttac tatattttaa attctttaat taatctatac aatatttgct
    67801 atatataaag tatatcaaat aacagcttca tttttttgta aatttacaga aagttaattt
    67861 tcattaaatg aatcatcttt attgataaac ataattccaa agaccactgc caaaataaaa
    67921 aaacatatta gtattgattt ttccacgaaa atttttgtaa aaaaagttcc taaaatagct
    67981 ccagatataa agaatataat aatagcataa tactgtaaac tcttttttat cataaaaata
    68041 ttcttagtat gtatataatg aaataaagtt tctgtagcac ttcttaaatt tccagtacac
    68101 attgtcgttg caaaactatt cccatttact tttctaaaac tctctacttg cattgaacaa
    68161 ataaaagaaa tagcaacatt tacaaacata ttaaatcttc ctctaggaat aaatgaaaca
    68221 ataagtattg taatcatttc aattataatg ataacctgac gccaatgaat ctttgtctta
    68281 ttatttgtaa aatactttct tatcttctcg gctataaata cacctaacat aaatgccaaa
    68341 ataggaatca aataataaaa tgattccttt acattacctt ctgctatatt aagcccaaat
    68401 aaaactatat ttccagtttg tgcattagca aagactttac ctcttaccac ataagtataa
    68461 atatccaaaa atcctcctgt tattgccaac aaagaacata aaaagaatga ttcagacatc
    68521 tgactcttgc ttgtcatata taaacctctc gctttctaat gatagataca aaatcaaaat
    68581 cattttagat tctatttttc attgatataa aaaaattatt tatatttaaa tattgtctat
    68641 atttatacat tatttgtatt tatatagcat tcatatttat ttatatttaa aca
//
LOCUS       DAYAPA010000002        68461 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.002, whole genome shotgun sequence.
ACCESSION   DAYAPA010000002 DAYAPA010000000
VERSION     DAYAPA010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 68461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 68461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68461
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.002"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            39..155
                     /gene="rrf"
                     /locus_tag="ACGI59_000056"
     rRNA            39..155
                     /gene="rrf"
                     /locus_tag="ACGI59_000056"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            275..466
                     /locus_tag="ACGI59_000057"
     CDS             275..466
                     /locus_tag="ACGI59_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HFG9737871.1"
                     /translation="MKKVALILAIIGALNWGAIGILGTDLIGSIFGGTYEMVSRIIYF
                     IVGLAGLYLIPSLMSDDRE"
     gene            621..2030
                     /locus_tag="ACGI59_000058"
     CDS             621..2030
                     /locus_tag="ACGI59_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFG9737872.1"
                     /translation="MFIINELNKIVNEEVISFHMPGHKKGQVYKKLGYKDILKNLYKM
                     DTTEILGTDNLHFPEGIIKNSQKKASRVFKSDYTCYLVNGSSCGIQSAIMSVCSPKSK
                     IITNRDCHQSVINGCILGDIDIEYVPCEISEETNVLKGISISNVIDIIDKNLDAKAVL
                     LTYPTYYGMTYDLEYICNYAHSKKMVVIVDEAHGAHLGLSERLPKTALEQGADIVVQS
                     THKTLPAFTQSSMIHIQGDRVDIDKILNMLRITESSSPSYLLLSSLELAVDIYENKGK
                     ELMEELLNNIITFKNKVDSNINIYDTNDKTKIFISSKNMGLTGYELENILRNKYNIQV
                     ELSNYYGVLLICTIGNTVEDFSSLETALNNIVKEDFKFEKLENKKYPVVIPKKILTPR
                     EAFYKTKKSVKIYDSIGKVCGECIVPYPPGISVISPGEVISKEIIDYLMFCHSKGMIV
                     SGLKDVDLNFIQIIDLEDI"
     gene            2161..2850
                     /locus_tag="ACGI59_000059"
     CDS             2161..2850
                     /locus_tag="ACGI59_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFG9737873.1"
                     /translation="MKGKLIIIESGSDASGKATQTEILYERLKKEGYMIKKVEYPNYE
                     SPSSALVKMYLKGDFGKNASDVDPYIASTFFTADRYASFKTDWEEFYNEGGIIIADRY
                     TTSNMVHQASKMDICDREEYLNWLADYEFNLFKIPQPDCVIFLDVPIEFSKKLMENRK
                     NKITGEEKKDIHESDIEYLEKSYNNALYIADKYDWKKINCVLDNKLRSIESIHNEIYK
                     IVLDSIKPMER"
     gene            2853..3788
                     /locus_tag="ACGI59_000060"
     CDS             2853..3788
                     /locus_tag="ACGI59_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFG9737874.1"
                     /translation="MYFENIIGQDFAKKYLTNSIKKNKLNNAYMFEGMDGIGKKKFAD
                     ELSKLLLDYENLENSPDYVLIKPDGNSIKIAQIRNLQSDIVIRPHKDYKIYIINNAEK
                     MTVEAQNALLKTLEEPPNYAIIILVTNNKESLLETIKSRCDIIKFSPIPIEDLKRYLI
                     NTGIEEERAQLLAIFSRGSIENALNLSQSSEFSMMREDIQQYIQIMLDKNIVEILNIP
                     NNMEKYRGKIIALLDMMINYFRDIILLKENVNKNMLINVDKLVFIQNMSGKISYSQLS
                     KIIDIIEDAKSKIKSNCNFNISIQVMSLNIYEVIK"
     gene            3785..4678
                     /locus_tag="ACGI59_000061"
     CDS             3785..4678
                     /locus_tag="ACGI59_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HFG9737875.1"
                     /translation="MIKIVGIRFKSAGKIYYFDPVDFNIEQDMDVVVETARGLEYGKV
                     VVGPKDMDESELVSPLKPIIRIATEEDKKVYLENKEKAKETFELCQQKIKEHELTMFL
                     IDCEYTFDRNKLIFYFTAEGRIDFRELVKDLAAIFKTRIELRQIGVRDEAKSIGGLGP
                     CGRKLCCSSWLGDFQPVSIKMAKDQSLSLNPTKISGICGRLFCCLKYEHDVYSEAIEK
                     MPVVGALVQSDDGKGKVVEVNPLLEQIKVEFQDKTVKIYTREDIKILREPKKCDGCGG
                     KCKDNDGLDEATLRELKKLED"
     gene            4756..5502
                     /locus_tag="ACGI59_000062"
     CDS             4756..5502
                     /locus_tag="ACGI59_000062"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HFG9737876.1"
                     /translation="MQLKDSERIDDLQLKGLKIIQDTNGFCFGIDAVLLANFTKIKKD
                     AKVVDLGTGTGIIPILIAGKSEAKKIIGVEIQEDVYEMATRSIKLNGLEERVEIINED
                     IKYIDKVLDINGYHVVTSNPPYMHIDGIKNPNDKKAISRHEVRCNLEDVIRAASRLVM
                     PRGKFFMIHRPTRLVDIITLGRKYKLEPKVIQFVHPRPQKAPNLVLVQFVKDGRPELK
                     ILDPLYVYGEDGNYTKELKAIYNNKDIGEL"
     gene            5503..6336
                     /gene="rsmI"
                     /locus_tag="ACGI59_000063"
     CDS             5503..6336
                     /gene="rsmI"
                     /locus_tag="ACGI59_000063"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFG9737877.1"
                     /translation="MSGKLYICPTPIGNLEDITYRTLRTLKEVDLIAAEDTRHSIKLL
                     NHFEISKPLTSYHEHNKDSKGDYLINKLIDGENIALISDAGMPGISDPGEEIIKQAIQ
                     NNIEIEVLPGATAFVTALVGSGMDTHRFVFEGFLDRDKKVRKAQLEELKEESRTIIFY
                     ESPHRLKDTLKDMLKILGNRNISINRELTKKYQEIIRENIETTIKIFEEKEIRGEFVL
                     IVEGFHGEKSKKENYDGLTDREYVLKLIESGMNKKDAIKIVCKDRKLKKDVVYKQVLD
                     L"
     gene            6359..7063
                     /locus_tag="ACGI59_000064"
     CDS             6359..7063
                     /locus_tag="ACGI59_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing metalloprotein"
                     /protein_id="HFG9737878.1"
                     /translation="MEIGKIIIESLKREDTHKIFIELDNKHELEKIIPKISCMKSVGE
                     CKYHVVNCFEHSINALKELEIVLNDKDFFPIHLRKHVSNYLNTYIEEGINKLHVLKLG
                     TFLHDIGKPDSMTLDETGRVHFTNHEKIGAQIIDNMGIKLELSTETYRLISKYVRYHM
                     ILLSLYKKNDLSRKELINVFNLVDEDTIGVIMLGYADIVSTVKLLGKSGEVSVLKTYM
                     EYILTNYLYKSNYTHV"
     gene            complement(7251..7679)
                     /locus_tag="ACGI59_000065"
     CDS             complement(7251..7679)
                     /locus_tag="ACGI59_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007287587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HFG9737879.1"
                     /translation="MDVTDYRIIEILQDDGRISMKDLGKIVGLTSPAVSERVKRLEES
                     GVIEGYKAIVNPDSLGRVIKAFIHISLPSNGYTEFIESAAKDPRIVECHHITGDDCLL
                     LKVIVKDMYELENVIDTIKKIGSTKTSVILSTPIQAKSIL"
     gene            complement(7716..9146)
                     /locus_tag="ACGI59_000066"
     CDS             complement(7716..9146)
                     /locus_tag="ACGI59_000066"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFG9737880.1"
                     /translation="MRNLTLLTDLYQLTMLNGYFEKNIHEDIVVFDMFFRKNACDGGY
                     TIVCGIDQVVEYIDNLHFSDEDLEYLKNLNLFSDKFLKFLKEFKFTGDIYAVEEGTIM
                     FPNEPLITVKAPLYQAQLIETALLTIVNFQSLIATKASRVCFAAQGDPVFEFGLRRAQ
                     GPDAGIYGARAAVVGGCAGTANVLAGKMFDIPIIGTQAHSWVQKFDNELEAFQAYADV
                     YPDKCLLLVDTYDVLNSGVPNAIKVFKNISEKGYKPMGIRLDSGDLAYLSKEAKKQLD
                     NAGFSDISITASNDLDEYTITSLKAEGATINSWGVGTKLITSFDSPSLGGVYKLAASC
                     EKGVLEPKIKISENPEKINNPGYKKVIRIYNEDNKAEADLIMLHDEVIDESKPLEIFH
                     PTYTWKTKVFTNYKVKELLKPLYIKGRCKYNKKAVLEIKNHVQYELSTIWEQYKRLSK
                     PHIYKVDLSRNLWYLKTQMIDSKKVL"
     gene            9278..9862
                     /locus_tag="ACGI59_000067"
     CDS             9278..9862
                     /locus_tag="ACGI59_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein A"
                     /protein_id="HFG9737881.1"
                     /translation="MIIIGKIWFYMVSIGIIGSLISKNMGELNKVILSETSKGIEFAI
                     SLAGIMALWMGIMNIAKESGLIEKIGQKLNPIMRRLFPSIPKGHKAMSYIVMNIALNM
                     LGAGNGATAFGLKAMSELQTLNNKKDTATNDMIMFMVINISSIQIIPFTIIKLRMDMG
                     AQNPSEIIFTTLFATIISTAVAIITCKFFQRRYR"
     gene            9878..10399
                     /locus_tag="ACGI59_000068"
     CDS             9878..10399
                     /locus_tag="ACGI59_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein B"
                     /protein_id="HFG9737882.1"
                     /translation="MEIFSNLLIPIIILYIVIYGKYKKIDVYDSFVKGAIDGLKAAWD
                     ILPYIIGIFLAIGIFKTGKGLDMLEWLFTPIANMMSIPKELIGLISVKPLSGSGALGM
                     YSELANRVGIGSLVEKMGATIVGSSETIFYTMAIYYGSLKIKNTRHTLSCAMISHVAG
                     VIAAVFICYVIFV"
     misc_binding    10465..10711
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            10795..12732
                     /gene="metG"
                     /locus_tag="ACGI59_000069"
     CDS             10795..12732
                     /gene="metG"
                     /locus_tag="ACGI59_000069"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFG9737883.1"
                     /translation="MSKPSFYVTTPIYYPSGGLHIGHTYSTVAADTIARFKRFCGYDV
                     KFLTGTDEHGEKIQKKAIEQGMSEIEYLDGMIKDIKALWNTMDISYDDFIRTTEKRHT
                     DIIQKIFTKLYEQGDIYKGEYEGRYCTPCESFWTESQLLEGNKCPDCGRETYLVKEES
                     YFFRLSKYEDRLKELFKDDSFCFPAARKNEMVANFLDKGLEDLSVTRTTFDWGIKVPF
                     DEKHVIYVWVDALCNYITALGYMTDNDEEFKKYWPANVQIVGKEIVRFHTIIWPALLM
                     ALGLEVPKQVFGHGWILFADDKMSKSKGNVVYPEPIIERYGIDTLKYFLLREFAFGQD
                     GSYTHRNFVTRINYDLANDLGNLISRTVAMVEKYNNGIIPTAKVSTDFDADLKEQAVS
                     TRENFEAEMDKMQFHEALESVWKLVRRTNKYIDETMPWALAKDETKKDELDTVLYNLC
                     ESIRIIATLINPIMNETANKIYEHIGIKGQDDITTWESTKTFGLIGENVKVFKGEPLF
                     PRLDVEKEIEELTKMFSGKPPVEEKPLEHKEEITIDDLDKIELRVGKIISCEKHPKAN
                     KLLVSQVKIGPETRQIVSGIAEYYKPEDLVGKEVTVVCNLKPVKLRGVESQGMILAAG
                     DDGDPYVLPFTQGAKDGCEVR"
     gene            12762..13532
                     /locus_tag="ACGI59_000070"
     CDS             12762..13532
                     /locus_tag="ACGI59_000070"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFG9737884.1"
                     /translation="MLFDSHAHLNDESFDEDRDELIGSLKDKGVDLVVNPGADIETSI
                     TAIELAKKYDFIYSAVGVHPHDVSKLDDTAIETLRKLATENEKVVAIGEIGLDYYYDY
                     SPREEQKEWFKKQIELANELKLPIIIHDRDAHGDTFEIIKNTKNPEIGCVLHCYSGNV
                     ELAREYVKMGCYISIPGTVTFKNNKKTREVVREIPLERLFIETDSPYMSPEPHRGKRN
                     NPSQVSFVADKIAQEKGISYEEVCRVTKENAKKFFNIK"
     gene            13870..14301
                     /gene="phnG"
                     /locus_tag="ACGI59_000071"
     CDS             13870..14301
                     /gene="phnG"
                     /locus_tag="ACGI59_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HFG9737885.1"
                     /translation="MYTVDSTKILINMNLEKLEKIYSMIKDTSSISIIKEPNLATVMV
                     RANESVKNTTFNLGEILVTECSVKVDESLGYGIVSENNNKKAIYLAVIDAVLHSNNSK
                     FDELKNYINKSVYEENLRYEQEIIDEFSLINKTKVQFNAMD"
     gene            14316..14891
                     /gene="phnH"
                     /locus_tag="ACGI59_000072"
     CDS             14316..14891
                     /gene="phnH"
                     /locus_tag="ACGI59_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HFG9737886.1"
                     /translation="MVNYTQEIYRKMLDSASFPGKLNNIDNINIENNTNLLNGAIGIA
                     ITLLDQEVTFYIENYTQKDIKNIRALTMSNQIDYRKSDYIFLDINSEVDILQIKIGSF
                     EYPDESATIVHQVNDLSTYSQTKYIKLDLSGPGIKSKNSIYINGIREEFIERLSSINK
                     DYPIGIDLILVDKKGEIAFIPRSSKLSWEVL"
     gene            14894..16009
                     /locus_tag="ACGI59_000073"
     CDS             14894..16009
                     /locus_tag="ACGI59_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-phosphorus lyase complex subunit PhnI"
                     /protein_id="HFG9737887.1"
                     /translation="MAYVAVKGGEEAILQAKNILEFYRVKGDSLPIDVKQIEEQMSYA
                     VDKVMSEGSLYNKELSALAIKQAQGDILEASFYVRALRSTMPRITYSEPINTEEMRIK
                     RRISATFKDIPGGQYLGATRDYENRILNVELLEEDEKKNRHFRDSFINELETVDEIPE
                     FTKISNIFRKENLLQEEVCENKLEEVIDDITRNSLRFPVSRAAKLQALTRGESGVVSG
                     FAYAIVRGFGDEHPYISELRTGNVFIKIKHPIYEDEEITIGDMLVTECEIVSKASQNK
                     TENSKNNSEDKNKMSLGYGLCMGNNENKAISMAILDKSLEPNSEKLAQDEEFVLLHID
                     GIDSLGFISHFKLPHYVDFKADVERIKKFEEVSLGDI"
     gene            15999..16868
                     /locus_tag="ACGI59_000074"
     CDS             15999..16868
                     /locus_tag="ACGI59_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HFG9737888.1"
                     /translation="MIYNYGFLDENTKKEIRRKILKAISIPGYQVPFGSRELPIAKGW
                     GTGGLQLTLSLIGKNDVVKVIDQGSDDSTNACNIRSFIASVSNVETTKDTLEATLIQT
                     RHRIPEEKLKSNQILIFQVPIPETLRIVEPSEVETRRMHSEEDYSRMWVYLYEDIVRF
                     NDISIAVEYPCKVNDRYLMNPSPIPRFDIKKLNMSDNLFLFGAGREKRIYAIPPYTKV
                     EPLEFEDYKFEEEKFEGKYCSLCNSTNTFLDEVYDSDTNEKYYSCSDTSYCEKVRLKN
                     NSIDVTIGGTWNE"
     gene            16861..17727
                     /locus_tag="ACGI59_000075"
     CDS             16861..17727
                     /locus_tag="ACGI59_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9737889.1"
                     /translation="MSKINDSKRLENIDKQVLIGRNINLIYGDGCEKCFESTGYESNN
                     ICKYCGSIVACNNVNLELYEGEVLGIVGESGSGKSTLLKILFFQEHANSGEVYISHYN
                     KDTNILELSDQKKRYIKNHFMGIVYQNPHLGLNLDFSSGGNIAEKLLMANLYNVEKIR
                     DRGKELLIKTNIPVERIDHKPKYFSGGMQQRVQIAKALSNNPPILLLDEVTTGLDVSV
                     QAEVLDLIREIQREFKISMIVVSHDFDVIKMLADRTIVMKNGSVIESGLTDQIMEDPQ
                     HPYTQQLINSLL"
     gene            17768..18481
                     /locus_tag="ACGI59_000076"
     CDS             17768..18481
                     /locus_tag="ACGI59_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HFG9737890.1"
                     /translation="MKETILEVNNFSKDFELHALNKTITACSNISFEVSKGEFLGIIG
                     KSGAGKSTILKSIYKTYIPTTGEIIFNSEIYGNVDLAKIGDREMINLRKKEIGYVSQF
                     LKTLPRITAIELVVHSLIESGFEKDCSYDMAKDILNQFEIKENLWDAYPNNFSGGEKL
                     RLNLAQAMVKKPRLLLLDEPTASLDNQSKIYVKEKMLELKSQGTTMIGIFHDIEFMET
                     VIDKTFTMTKGTISESGVA"
     gene            18482..19651
                     /locus_tag="ACGI59_000077"
     CDS             18482..19651
                     /locus_tag="ACGI59_000077"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HFG9737891.1"
                     /translation="MIKLIHNANIVLEDMILENGHLIIEGDTIKKVSNDKIDYAFDFN
                     EIDEIIDAKNLYVIPGIIDIHSDAIEKEIEPRPSTLLPFNMAFYELDKKLPANGITTV
                     YHSISLGDGVGVRSIDNSLKMIKNIDSYKNIDSKSINHKVHLRYEVLYYEGLEKVLEL
                     LDENKIDYLSIMDHSPGQGQYTNPTFYKEYATKVWGVTENYVDTWLDDLVNLHDNLDW
                     NKIANIIGIAKTKNINVASHDDDTLEKMDFIKNIGINVSEFPITLEVAKHSKKLGVLT
                     CLGAPNIVRGKSHNNNLKAMDAILEDCCDIVCSDYLPSAMIKSMCIVAERINNLNKAV
                     SLFTSNPAKAVGIYDERGSIKENKKADLVLVDIDSEYPKVVNTIVNGKTVYKREV"
     gene            19655..20491
                     /locus_tag="ACGI59_000078"
     CDS             19655..20491
                     /locus_tag="ACGI59_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFG9737892.1"
                     /translation="MLTELHCHTSISDNNFTTEEIIKKAAEKNINILAITNHDTLSGL
                     DEALVLGEHYGITIIPGIEISAYDYKNRKRVHILGYNIDLNSQEIKNLCNPMVCDRHK
                     ASVKMVNKIIDLGYKISLEDVKKYSSKTGIFKQHIMKALIDKGYTDNIYSSLYKELFH
                     RGNGKVYVSLKYVDYRDAIKAIKNSGGICVLAHPGQMDNFSAIEEMVKVGLDGIEVYH
                     PSHNKELEKESLSYAKKYNLVVTGGSDYHGFYSQHNYELGSKSLNKEDLFKFRLALGG
                     VL"
     gene            20492..21487
                     /locus_tag="ACGI59_000079"
     CDS             20492..21487
                     /locus_tag="ACGI59_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9737893.1"
                     /translation="MSFLQVKNVGKSYGQVKVLKDISIDIEKGEFICLLGPSGCGKST
                     LLRIIAGLEDKHGGKIIINDKDMTNSPPESRNFGIVFQSYALFPNMNVYKNIAFGLEN
                     KNISKSNIDKKVKEVLEVVELSGYEKKYPSQLSGGQQQRVALARAIALEPDFLLLDEP
                     LSALDAKVRLKLREQIRSLHRKLGITTIMVTHDQEEALCLADKMVVMNRGEIIQVGTP
                     KEVYKNPETPFVADFIGTINFIDDGINKIAIRPEDIKVESNRDSKDKDIKVGEILDIE
                     FRGFNYRITVEYRSKQMKLDVVSKVAEQMKLCIGSKINFKIPKEGIVQYKSEGCA"
     gene            21505..23193
                     /locus_tag="ACGI59_000080"
     CDS             21505..23193
                     /locus_tag="ACGI59_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033225 - ATPase-coupled
                     2-aminoethylphosphonate transporter activity [Evidence
                     IEA];
                     GO_process: GO:0033223 - 2-aminoethylphosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     permease subunit"
                     /protein_id="HFG9737894.1"
                     /translation="MITSKNKRRISDSTIQKYFLIFSVIVLIVFILFPLVSLLKNAFM
                     DTSGNFIGISNFSKYVENPSLIASFKNSIFVSTVSSLISLCLAFIYSYAITRTNIKFK
                     EFFKTMGMFPLFAPTMLYGISLIYLFGNKGIFTTMGLEVPLYGPLGIIISQILFTFPQ
                     AFLILSVALSMADYRLYEAADSLGASEFKKFCKITIPGIKYSLLSSFFVSFILAFTDF
                     GAAKVVGGNYNVLATDIYKQVVGQFNIPMGATVSMVMLIPVLIAFTLDKMASKKQGMT
                     ITAKSMPYKIKENKFRDRVFLIVCSFITLLIFILIFVSVIGSVIKLWPYNLSFTMEHY
                     TFKGILGSGIKTYLSSLKISTLTAIFGTIFVFFSAYMIEKVDKFPKLRQTAYLLSMIP
                     MALPGLVLGISYITFFNSASNPLNFLYGSTAILVIVNVVHFYSVCFITSNSSLKMLDK
                     EYELVAKSINIPFYKVFFNITVPMSITSILEIVVYYFVNSMVTVSALIFLYTPQTQTA
                     SISILKLDEIGYIGPSAAMAVLVLLTNIIVRLLYEFVTKKLKNNTQKWQQKDVA"
     gene            23205..24272
                     /locus_tag="ACGI59_000081"
     CDS             23205..24272
                     /locus_tag="ACGI59_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033225 - ATPase-coupled
                     2-aminoethylphosphonate transporter activity [Evidence
                     IEA];
                     GO_function: GO:0033226 - 2-aminoethylphosphonate binding
                     [Evidence IEA];
                     GO_process: GO:0033223 - 2-aminoethylphosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HFG9737895.1"
                     /translation="MKFKKLVSLGVLSTLLLSVITGCSTDSSGSDSKSDSKTKGDLTV
                     YTAIEEDSIEPYLATFKEKYPDIKLNIVRASTGDITARLLAEKDNPQADVVWGVAATS
                     LLVADDQGMLEPYAPKGSEEIESAFKDDKEVPSWVGIDAWMTGITVNTKELSDKNIPV
                     PSSYEDLIKPEYKGLISMPNPSSSGTGYLTVSALIQIMGEEKAWQYMDKLHENIGVYT
                     QSGSAPATSAASGEYPIGISFGYRGIKLKEEGYPVEVVFPKEGSGWDIEANALVKKDN
                     IKEASKVFLDWAISKDAMNEYSKNYAVTTISTGNPIPEGFPKKPLEQMIDNDLKSAAK
                     NREDILNKWISKYDGKTEKES"
     gene            25036..26103
                     /locus_tag="ACGI59_000082"
     CDS             25036..26103
                     /locus_tag="ACGI59_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033225 - ATPase-coupled
                     2-aminoethylphosphonate transporter activity [Evidence
                     IEA];
                     GO_function: GO:0033226 - 2-aminoethylphosphonate binding
                     [Evidence IEA];
                     GO_process: GO:0033223 - 2-aminoethylphosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HFG9737896.1"
                     /translation="MKFKKLVSLGILSTLLLSVITGCSNVSSGSNLKSNNKTKGELTV
                     YTAIEEDSIDTYLATFKEKYPDIKLNVLRLSVGGITARLLAEKDNPKADVLWGVAATS
                     LIILDDKEMLEPYAPKGFDKVNSAFKDDKKVPSWVGIDAWMTGITVNTKELKAKNISA
                     PSSYEDLIKPEYKDLISMPSPASSGTGYLTVSELIQIMGEEKAWQYMDKLHKNIGVYT
                     QSGSAPAILAASGEYPIGISFGNRGIKLKGEGYPVEVVFPKEGSGWEIESNALVKKDN
                     IKEASKVFLDWAISKDAMNEYSKNFAVTTIDTGNPVPEGFPKNPLEQMIDNDLKSAAR
                     NREAILNKWIEKYDGKTEKES"
     gene            26295..26825
                     /gene="rnmV"
                     /locus_tag="ACGI59_000083"
     CDS             26295..26825
                     /gene="rnmV"
                     /locus_tag="ACGI59_000083"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HFG9737897.1"
                     /translation="MIKEIIVVEGRDDVTAVKRAIDAELITTGGFGFPKGVMERIKAA
                     NERRGVIIFTDPDFAGEKIRKKIASEVPGCKHAFLPREEAKKDGDIGIENATPQSIIA
                     ALNKVRTENTEKRDEFKQVDLIRNGLIGNEEASLRRDALGKILGIGYGNAKQFLNRLN
                     NYGVEREEFIDALKQI"
     gene            26996..27865
                     /gene="rsmA"
                     /locus_tag="ACGI59_000084"
     CDS             26996..27865
                     /gene="rsmA"
                     /locus_tag="ACGI59_000084"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFG9737898.1"
                     /translation="MDRLSSHNATKEVVQKYNFKFSKSLGQNFLIDSNIIDKILSGAR
                     ITRGDNIIEVGPGIGTLTREMGKIAEKVVAIEIDRNLIPILKDTLSDLDNTEVVNQDI
                     LKVDIQELVKDKLNGGPVKLVANLPYYITTPIVMKFLEEDIPVTDIVVMVQKEVADRM
                     NAIPGTKDYGALSIAVQYYCDTEIVAKAPRHMFIPQPNVDSTVIGLHVRDKRKYDVHN
                     EDIFFKTVKASFGQRRKTLLNSLGGLGFLNKDEIREVLKEANIDEKRRGETLSIEEFS
                     VLSNIINTKVSSK"
     gene            27931..29193
                     /locus_tag="ACGI59_000085"
     CDS             27931..29193
                     /locus_tag="ACGI59_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9737899.1"
                     /translation="MGSKRKFKRDYIILEAKDINFRYKERVLPKAFAKVEVNDEKAII
                     ALYVENLKYVKDGYKAVAIKDDHGLIDLGNIILNEQGRGEFVLDLEDYDDEIKGIALL
                     YERNIPLIGFKGNKLENYEDILFQEVEEEEYEEYEELEEEDEEIEENEESDRDEDSEE
                     EYIIEEYDEDEYEDYWEDDEDIEEGELKARTEVEDSDDEEIEEFNNYKEIENYNRVKV
                     QEEYIDIEYDEEEEEEEVFEVNREEDRFVSSKPTKSKKVQKDQNNIEKIKRANGVSQG
                     ESGNKQTSTLLMPRQIKKGLKLFKEVKPFVTDYIDKTRWWKIEINPTTLCGYTMPYLG
                     YLNVLNYTMYSDAVLHSYKYRHYLFGVQYDEYNKRKHYIYAIPGNRNEQPDNGSTGFL
                     RYQACDNRTNSLGYWLCFIDSRQRNIVR"
     gene            29344..30459
                     /locus_tag="ACGI59_000086"
     CDS             29344..30459
                     /locus_tag="ACGI59_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HFG9737900.1"
                     /translation="MINEQRILNEFLELVQIDSLSLKEGNVAKVLVKKLEEIGCSVVI
                     DNAGEKANGETGNIIATLKGNKEGKKILFSSHMDTVTPGIGVKPIVDEANGIIKSDGT
                     TILGSDDKAGIAAILEGLRYINENNIEHTDIQVVFSICEECGLVGAKNLDYGKIDSEY
                     AFILDSGGSPGEIIVKAPAQDVINVKILGKTAHAGLEPEAGISAIMVAARAIENMNLL
                     RIDEETTANIGIINGGTATNIVTGEVNIVAEARSLKENKLDVQTKHMVETFEKAAKDF
                     GAQIEIDVNRAYTPIDVSEDSEIIKLAKKAFSNLGIEGHTESTGGGSDTNILSKNGIE
                     AITLGIGMKNAHTLSEHIAIKDLYDSAKMVVEIIKEA"
     gene            30645..31541
                     /locus_tag="ACGI59_000087"
     CDS             30645..31541
                     /locus_tag="ACGI59_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFG9737901.1"
                     /translation="METRYEEANKITIQSILWNIVLTIIKVIAGVIGNSSAMIADGLH
                     SASDIISSIGVLIGNYVSSRPGDREHNYGHEKAETLVSFVLSILLIFVSITIGIEAIK
                     SLFNLDALSVPSILPLVVSVISILIKEYQYRITIKVAKKINSPALKADAWHHRSDALS
                     SVAAFIGIGGSILGFKPLDPIASVVVAIFVAKVGISILISSVNELMDVSVDEEEIKEL
                     KFIVADTEGVKNLGDIKTRKHGAMAYVDLTICVDENLTVKQGHDIATKLEKHIIKHME
                     FVKGITVHVEPCTNCQGNKCNN"
     gene            31611..32408
                     /locus_tag="ACGI59_000088"
     CDS             31611..32408
                     /locus_tag="ACGI59_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9737902.1"
                     /translation="MSKKRKLKKKAAILIGVVVFFIIYFISFRIGINMEKDKSEVPKK
                     VEKEEEDNRSLRTQIKEKKKVYISDKNIENIKIEEDMWDEIKLFFSDFKKVRNTTSSY
                     EAVYEGYSDDGVKFSTDLNFFRVYTVNKEEYYKVPVSSKDAFKKLLDESIYTSFDLLS
                     HYKDWKEVTITHGKEIKKVHRWKFDDLAHKMISKRVVGKVQPEKSKERSDYNFTINIK
                     GDNFDVKVDTMGKDYVKVTSKKNVSYYEVHTSLFDYLKNEIFEIGKK"
     gene            complement(32463..33815)
                     /gene="murC"
                     /locus_tag="ACGI59_000089"
     CDS             complement(32463..33815)
                     /gene="murC"
                     /locus_tag="ACGI59_000089"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFG9737903.1"
                     /translation="MNIHFIGIGGISMSALAEICINKGYQVSGSDSNESYLLDKLRDQ
                     GATIYIGQKKDNISDDVNMVVYTAAVHPDNEELVAAKEKNKLVMNRATFLGQIMREYK
                     NSIAVSGTHGKTSTTSMLSTIFEYADLDPTILVGGNLSMIGGNVKIGNSNHFITEACE
                     YVDSFLNFNPKISIVLNVEEDHLDYFSGIDEIKASFNKFGKLLPPEGYFIINGDDENI
                     DDILYDVKATIIKYGRDSDNDAVIKDIHFDNSGHGIFRIEYEGRDLGEFELSVYGLHN
                     IYNASSAIMAALVSGIDLETIRKNIKIYKGVGRRFETKGYYKNALVVDDYAHHPTELK
                     ATLAAAKKLKKSTLWCIFQPHTYTRTKSLLGEFSEAFYAADKVIITDIYAAREKDPGD
                     IHSKDLVEKLYQNNVDAIYIKEFEDIVKYLRENVKDNDLVITAGAGPIYKVADLLVEK
                     "
     gene            34034..34912
                     /gene="purR"
                     /locus_tag="ACGI59_000090"
     CDS             34034..34912
                     /gene="purR"
                     /locus_tag="ACGI59_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0045982 - negative regulation of purine
                     nucleobase metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HFG9737904.1"
                     /translation="MKFKRTERIGAIVKILSDNPNKIYTLSYFTNQFNAAKSTISEDL
                     LVVKNVFEKLHLGKVITISGAAGGVKYIPKTSIAENAEFLMELCERICDKSRILSGGF
                     LYLIDLIYDPTIAAKIGKIFASNIEYVDADYVVTMETKGIPMALMTAKAMNLPLVIIR
                     KDIKVSEGPTLSMTYVSGNSSKVESMSLPRKALKPDSKVIIIDDFMRGGGTIKGMVDL
                     MNEFGAEVIGTGVFISTTNPSEKMVKDYISLIQLDVNGDKIVVEPNLKTFKDEYRNED
                     LDEEEDSEFEFEIDED"
     gene            35068..35346
                     /gene="spoVG"
                     /locus_tag="ACGI59_000091"
     CDS             35068..35346
                     /gene="spoVG"
                     /locus_tag="ACGI59_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030435 - sporulation resulting in
                     formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HFG9737905.1"
                     /translation="MKITDVRVRKLTEEGKMKCIVSITFDNLFVVHDIKVIEGHNGLF
                     IAMPSRKVGEGNFRDIAHPINAEMRQVLEDAVLQAYHEALVQWEVAAE"
     gene            35559..36938
                     /gene="glmU"
                     /locus_tag="ACGI59_000092"
     CDS             35559..36938
                     /gene="glmU"
                     /locus_tag="ACGI59_000092"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFG9737906.1"
                     /translation="MNFKAIILAAGKGTRMKSKYPKVIHKVCGKEMVNHIIDVSKKSG
                     VKDTVVILGHEADVVKEKLAEEIIIAMQTEQLGTGHAVKMAKEYINDEDTIVVLCGDT
                     PLIKEETLKRLFEYHIENKYHATVLTTRVGNPTGYGRIIRDKKGDLLKIVEQKDANSE
                     EKMISEINSGIYCFNGKSLREALDLLNNNNSQGEYYLTDTAKIMRDKGLKVGAFAGST
                     IEELMGVNSRVELSKAEEIMRRRINESHMVNGVTIIDTNSTYIESDVMIGNDTIIYPG
                     VMLQGKTRIGSDCIIGMNSSITNSEIGDGTEIKNSTIIDSKVGENSTVGPYAYLRPKS
                     DLGNNVKIGDFVEVKNAIIEDGSKASHLSYIGDAHVGKNVNIGCGVVFVNYDGKNKFK
                     SIVKDNAFIGSNSNLVAPVVVEEKGYIATGSTITHDVPDGALAIARERQVIKEGWVEK
                     KNQKDDQSK"
     gene            37033..37983
                     /locus_tag="ACGI59_000093"
     CDS             37033..37983
                     /locus_tag="ACGI59_000093"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0002189 - ribose phosphate
                     diphosphokinase complex [Evidence IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFG9737907.1"
                     /translation="MNTSGSEIKIIAGNSSKELAQKIADYIGVSVLDCEVGTFSDGEI
                     CVNMNETVRGCDVFVVQSTNSPVNDNLMELLILIDALKRASAGRITAVIPYYGYARQD
                     RKAKARDPITAKLVANLITAAGADRVLTMDLHAAQIQGYFDIPLDHLLGGTILANYFN
                     EKKIEDLVVVSPDLGSVTRSRKFANTLNGEVPIAIIDKRRPKANVCEVMNLIGDVKGK
                     NVILLDDMIDTAGTIVNAANALKEFGAKDVYACCTHGVLSGPAIERIANSEISELIVL
                     DTIQLPEEKRIDKIKIKTVAPLFGDAIRMIFSNESVSKLF"
     regulatory      38821..38906
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            39068..39589
                     /locus_tag="ACGI59_000094"
     CDS             39068..39589
                     /locus_tag="ACGI59_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HFG9737908.1"
                     /translation="MKLKKNKKGFTLVELLVVIAIIGILAVVAVPALFSNINKAKVAS
                     VESDYSSVKSAALSYYSDTNKIPVTPDGQTGLSVLETYMESLPDKADIGGKYKLIKVG
                     NKLVLQIGTNTEGVTLTEAQSAKLLSDIGENKIYTSATTNSLGNPLTSNTKIDNNVLY
                     IVLIDNTVMDTTK"
     gene            39740..41416
                     /locus_tag="ACGI59_000095"
     CDS             39740..41416
                     /locus_tag="ACGI59_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HFG9737909.1"
                     /translation="MAKKVRIGDKLVEKGYITEEQLKWALSEQKNSGKRLGEFLVQEG
                     LIDSNLLISVLKELLDIESIFLEGTEIDTLATKMVPENICKRYTVFPFKIDGNKICLA
                     MSDPQDREAVQDVRRMSGKDVEIFISSTEDINKAIGHAYAHSEINKAMTEYNKNRTGG
                     VRETVILEEDVNAAPIVRLVNNILENAVRMEASDIHIEQSENYMRVRFRIDGMLREYM
                     RMNSAPYKAVISRIKIMSDINISEKRIPQDGRIYLKVDNKPIDFRVSTMPTNRDEKIA
                     MRVLDKSNFMVSKEVLGIDEHGSKIYDELINTPYGLILVVGPTGSGKTTTLYSMLNQL
                     NTENRNLLTIEDPIEYELPGVNQSQINEKAGLTFASGLRAFMRQDPDIIMVGEIRDTE
                     TAEIAIRASLTGHLVLSTLHANTAVGAISRLLDMDVESFLITSSVLGVISQRLTRKIC
                     EHCKVSYEADIGEKKALGIDVNESVTIYRGKGCERCNNTGYKGRLGIFEMLEITPEIK
                     ELIDSSANQREILKMARKQGMVSLKEDIVKKVLNGKTTVEEMIRIILMTD"
     gene            41428..42636
                     /locus_tag="ACGI59_000096"
     CDS             41428..42636
                     /locus_tag="ACGI59_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HFG9737910.1"
                     /translation="MKSFKYTVIKEDGKKQTDVIEANDFNEARNLLRKRNLRVIEIKE
                     SKNKNIRLSSRKRKKDLGADQISHFCRQFAIIVSSGINSISGLETLARRSTNITLREE
                     INRIVAEIKVGSTIADSMLSSKSKFPKLLGAMVATGEATGKLDEVLKSMASFYASEHR
                     VKQKLRNAATYPLIVLISSFIMIFIFTTFMVPKMINSITTVGATLPLITRIVMGFGMF
                     MKKFWIIVLLVIILFIYQFKKYLKTSVGRAHKDRVINKIPVLGKGINCMVATRFSRAL
                     YLFVSTGYPLVQGLDYIIDSVNNTMAEKILASAKDGITRGEGLAENLERYTYFDSVLV
                     QMIAIGEQTGELENISRQMAEFYEYESEIYLNRMASMIEPVLIIAVGIIVAILVVSIF
                     MPMLSIYDAM"
     gene            42651..44363
                     /locus_tag="ACGI59_000097"
     CDS             42651..44363
                     /locus_tag="ACGI59_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HFG9737911.1"
                     /translation="MNKVYVSYYGDSISIVEGRYKHDKDKFYVKNFNVFSIEDIAQDF
                     SKEKYALLRYALENVKLKSRHVVFCLNTRDVILKPQKLPNMDKKDLDGFMKLEMDEMM
                     ALESDEYVFSYEVSSETPDNSDGDESSLNLIIGAIQKEEVNAIVEIMHEFDLVLDRID
                     TLSTAYLRMLKNLDYNDIMVTNISDNSTIINIYKKDTLFISDNIPIKITAKDMDMQLL
                     NVVEESKGLMNYYSSRNFGKVTDTILILGKRYSNKLIYETFSSAFSSYVSQGLTEIMD
                     ISDMITGDIDEDDINSIVELLGSMIEPKGKRDFENINFLPMDILRKQIKAKQMKNILK
                     IAPIVILILAIPYLCLIFSNNLANKELENVNGEIRNIESAYYQIGNIEKMIQSKSEEI
                     KVYDMLIGKKVKWENLLDDIDKNTPSKVELTSLSTTYQNLGTNNKADNSKSNKTTTDK
                     SSNNETKSSTKSDSNGDKTDNDLNEDKTTNVSEALTGNSETNSNNTDKDDKTPLYDKI
                     PNVINIEGNAKDSSYVGQFLYKLKGLYYFKDVKLHSIKSNAEDSNYTFSMTLYLKEGV
                     LTNE"
     gene            44356..45264
                     /gene="pilO"
                     /locus_tag="ACGI59_000098"
     CDS             44356..45264
                     /gene="pilO"
                     /locus_tag="ACGI59_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HFG9737912.1"
                     /translation="MNKDIFKKDIKDIFKMEVSLKGILIKVIILLAILSYGYFGLYPQ
                     YQKYIATKNSVKQATEKLNIYKEKTALMPKYEKQLEEVESELKEKSKNLLYDMQDGLF
                     LIGLDKKMKAYNIDLTSYDVGNVKEYEHFYGISTTISVRGDYRNVKKLMAYLEEQKNV
                     TQILDYTMTSYIEPEKEEEETTNNKDLIKNVNINKDTKIYWVEGENKWHLYKDCPLIK
                     EGEILKNDKLLDRSTNYLCNTCVLRAATEKEAKKEVVAPKAEGVVDATFKFIMYTKEN
                     TTMILDTDNPSSWRPGKYNPFKSSAK"
     gene            45291..45857
                     /locus_tag="ACGI59_000099"
     CDS             45291..45857
                     /locus_tag="ACGI59_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HFG9737913.1"
                     /translation="MSLYKNNEKGLTLLEVIIAVFILTIVLSISYKVFNGITSAVKKQ
                     QIITDAQVNINLINKYLNRDLENCKELTKTGSGNNYEYNIEMPDNVVKYEVSIETKKN
                     TEVYSVTRIQNNTIDTENEVREEIIYNQPLVQNNKEMKETPFKIEKQTGKSIYTVSIY
                     YNESVQESHKNSNLNNKTYTFDVMSRIG"
     gene            45894..46421
                     /locus_tag="ACGI59_000100"
     CDS             45894..46421
                     /locus_tag="ACGI59_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HFG9737914.1"
                     /translation="MSKKNSERGFSLIEVLVAMAIMGIVLFAFFNIINTNNKANTKND
                     TDITSLNYVQSEIENLREKIKSGEFDFDSLDKLEDGTVVYEKLIDKSKKVVYDKVLSE
                     GDVSLYDTPYEKITTIKDEDGNLIDKGNITNKIKTIVEDKSGQIYKIAVTGKSMNDYS
                     SKKEVKIVTEIFKDK"
     gene            46442..47980
                     /locus_tag="ACGI59_000101"
     CDS             46442..47980
                     /locus_tag="ACGI59_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9737915.1"
                     /translation="MRKWNKFKSERGAALVLVLVVVALLSIVGLIFSNQIANRIKSTK
                     TTNEGIQAKYLAETCVENSIDKAYEKLYDELEKMDNEFKSENQEKSISRSKLRDISDE
                     DFNNQDEENIEAERLGYMNNINFYLNKASSDLEKASMELKKLYDLDMLDYRDIEYVDA
                     NIISHRDSILEICKNYTSGDISKINEYILKEDIDSTTLIEAKLVNNDILLKMFLEENK
                     IENEHLNSAFSHTYKALDNISLAMQNMIEYRHTFHIDEPKVEVSNGIPDSQQYYELIQ
                     NPIINSMEYIWNSKWDTLEKLLEILPNQTQGFNSLRVHLRNNVRKFEKLSDNISSGKK
                     NTAKNFLKYKELLYEISDQCNQLKSMSYEKIPVKYDNMALITTFDYIQNELLAEIKCR
                     LKELKPQEIDKTEGITIKIPFYKADYDMTKEGWPKLKENGSGAELSLMVTGDKDGIKE
                     VEVTDGKKNIIGLGVEENSNSKYKVDAIVNFNLNIDTNVVGNYDIKDKILINHDISSY
                     KKVN"
     gene            48080..49162
                     /locus_tag="ACGI59_000102"
     CDS             48080..49162
                     /locus_tag="ACGI59_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HFG9737916.1"
                     /translation="MYIYDLLEQGIRLNASDIHITVGTNPVARVKGGFVKLSEQILTS
                     EVTMQMAKDIAGESMFKVIEEHGEADFSASLKTGERFRVNAYRQKGNYAIAIRTITAE
                     IPTFEKLGLPESIKSFTEKHKGLVLVTGPTGSGKSTTLASMINIINEKQQKHIITLED
                     PIEYVHHHKQSLVNQREVGTDTESFHSALRAILRQDPDVILIGEMRDPETVSIALTAA
                     ETGHLVFSTLHTVGAAKTIDRIVDMFQPSQQQQIKTQLSTVCEGVVSQQLLPTADGKG
                     RIAAIELMFATPAIKNLIREGKTYQIPNMIQTGVKVGMKTMDQDLMELYKNGKITKDM
                     ALSRCTDQEFMTRMIGGVNYNGYYNR"
     gene            49143..49925
                     /locus_tag="ACGI59_000103"
     CDS             49143..49925
                     /locus_tag="ACGI59_000103"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFG9737917.1"
                     /translation="MDIIIVNLYVFMVGIVFGSFFNVCIFRIPEGISISRPPSHCMSC
                     NTRLKPIDLIPVLSYLMSGRKCRYCYEKISSRYAIVELITGLLFLTVFMLYGISTSTI
                     YYLVLVSLLIIITFIDIDHFIIPDKILVFGAIFSMIFNLLFKEIPVKDSILGVFICGG
                     SVWIIVLLIEFVIKKECMGGGDIKLFAMLGFYMGVKNGLLTALLSVYVGAVYGICVII
                     ISRIKGKEYNSVIPYGPFISIGALITILCGKQLLELYFSMVI"
     gene            50207..50677
                     /locus_tag="ACGI59_000104"
     CDS             50207..50677
                     /locus_tag="ACGI59_000104"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HFG9737918.1"
                     /translation="MVLIPFLIIISIVDYYTIYVYDITIVSGIIVQGIIFLVFFNIVQ
                     FDYINHIFGLLIGFIIPYIIAKLTKGLGYGDVGLYSLCCFALGNNYGFYVIVLSFILA
                     FLFFIILYITKIFKDVDIRKQIPFTPFISLSTILIIFTEYNIFNFYYNLLYKFI"
     gene            50691..51251
                     /gene="pth"
                     /locus_tag="ACGI59_000105"
     CDS             50691..51251
                     /gene="pth"
                     /locus_tag="ACGI59_000105"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFG9737919.1"
                     /translation="MYVVVGLGNPGKKYEKTRHNVGFDVIDILAKEYNISVTKIKHKA
                     LIGEGRVGTEKVLLVKPQTYMNLSGETLIDIYKYYKVDLSNIVVVYDDIDLEVGKIRI
                     RKKGSGGTHNGMKSITKCLGSNDFPRVRVGVSKPEAGQDLADFVLSRFRKEESDNINE
                     ALEKAADAIDSIIRENIDMSMNKYNG"
     gene            51268..54654
                     /gene="mfd"
                     /locus_tag="ACGI59_000106"
     CDS             51268..54654
                     /gene="mfd"
                     /locus_tag="ACGI59_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFG9737920.1"
                     /translation="MNDVFLYPLQNSKEYKDIINCIKNTKGSLLVNGLLPVQKPHISY
                     SIFNDLSRQMIFITSSDLEAKKVYEDLSFYMEDKVEYLGFQDIYFYHLDAKDRNEEAK
                     KLKVLLKLANKEKIILVTSIEAVLRKYIPKQVLLDSVSHYKVGDSLDLEKLTEKLVSL
                     GYERVSKIEGFGQFSIRGGIIDVFSLEYTNPIRMELFDDEIDSIRTFDVYSQKSIDKL
                     QQFSITPSREFIYPEKTTDALVKLKKETTKNTDEGTFQNIDYISSKTYFEGVENYIDY
                     IYPEENKSIFTYLADDAIVFINDITRLKERCENYINEFRENYKLNLERGLAIKNQGKL
                     LYHYTDLEYLVKDKSVVLNSLLPKSINNFSIKSIINFESREVPTFNGKVDLLVEELNR
                     LKYNGYKIILATNTLERANKLGKDLLDKGLETTISKDRDIEIKSSQVIIVPAHINSGF
                     QYKSIKFVVITDNEMIGVYKRASKTSNKKVKKGKKIESFLDLSVGDYVVHENSGVGRY
                     TGIEQITVNAIKKDYMKIVYQGGDNLYVPIDQMDKVQKYIGAEVEKVKLNKLGTNEWT
                     KAKAKVKKEIEDMTKDLIELYAKREKIQGYKFSKDTPWQAEFESLFPYQETEDQLKAI
                     EETKKDMESSKVMDRLVCGDVGYGKTEVAIRSIFKACMDQKQVAVLVPTTILAQQHYN
                     TFKERFENYPLRVEVLSRFKTPKQQKQIIEDAKKGLVDILIGTHRIISKDINLPNLGL
                     VVIDEEQRFGVKHKEALKKIKSTVDVLTLSATPIPRTLHMSLSGIRDMSVIEEPPQER
                     HPVITYVTESKESVIQDEIERELSRGGQVFFVYNRVEHIEEMASMIQKLVPDARVAVA
                     HGRMTSKSLENIILGFLNKDYDVLVCTTIIETGMDISNANTMIIYDADKMGLAQLYQL
                     RGRVGRSSRQGYAYLLYEKDKTLSEIAEKRLKAIREFTEFGSGFKIAMRDLEIRGAGN
                     ILGSQQHGHMAVIGYDLYVKMLNDAIKKVKGEPIVEEIDVEIDLSVNAYIPDNYIKDE
                     LIKIEMYKKIASIENKEDMLDIQEELEDRFSDIPKPVQTLLTIAYIKSLCKILKIEKI
                     RQLKDEILLVPITKYRTKQKIGYNIVTELEELLEKMCKVK"
     gene            54685..55680
                     /locus_tag="ACGI59_000107"
     CDS             54685..55680
                     /locus_tag="ACGI59_000107"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFG9737921.1"
                     /translation="MKKVITLVIAMILVVSVTACSSSKGETVATVEGTKISSDEFKKT
                     IALYKDSMEQTYGKDIWDKEVEKGVKYKDKFKDLILDQLITTEVIYSQAKKDNLLPKK
                     EDVEKSFKELKDAMGKDEKYKEQLKKLGIDDEFLRDQQEKDLAMQNYQSNFAKKTKIS
                     DEEMKKYYDTHKDEFKKDEVEASHILLKTVDDNNKPLSDKEKAEAKKKAEEALKEVKS
                     GEDFAKVAKKYSQDTSASDGGKLGFFSRGQMVAEFEDAAFSMKKGEVSDLVETQYGYH
                     IIKVTDRINEQTSFEDAKETIKDQLLKNKYQEQIEKLTKEAKVEKDEKVINKITI"
     gene            55804..56343
                     /gene="spoVT"
                     /locus_tag="ACGI59_000108"
     CDS             55804..56343
                     /gene="spoVT"
                     /locus_tag="ACGI59_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein T"
                     /protein_id="HFG9737922.1"
                     /translation="MKATGIVRRIDDLGRVVIPKEIRKTLRIREGDPLEIFTAKDGEV
                     ILKKYSPIGELNEFSQEYAETLAEVLGYGVLITDLDSIIAVSKLPKKDYKEKSISDEL
                     EAIISERTVKYSKDKNILPLFKEDQKEYTSQVIMPIISASGDCIGSIVVVSKDKDTIA
                     ESEEKSLKIAANFLGKQVQ"
     gene            56463..58076
                     /locus_tag="ACGI59_000109"
     CDS             56463..58076
                     /locus_tag="ACGI59_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFG9737923.1"
                     /translation="MNNNKNKDSFLKGALILGLAGVIVKIMGGFFRIPLGNMIGSEGM
                     GYYQAAYPVYTLFLTLATAGFPTAVAKLVSEKVAIGNFKGANKIFKVSHTVLFITGII
                     SFCILFFGADYIVTNVMKNPGALYSMKAIAPALLFVPAMSAYRGYFQGRQDMTKIAVS
                     QVAEQFFRVVLGLTLAYLLMKSLGQTFGAAGAISGATIGSIASMLYLVFAYMLGRKER
                     RAEIDASQRFKDERVSYIFKKLLTVAIPITIGASVMPLVNMIDNVIVIRRLMEAGFTY
                     KVANSMFGQLTGMAMATINLPAVITTAMSMSLVPAISKAYALGNKSKARKDTKSAVKV
                     TLLIVLPCAFGMASLAIPIMGLLFPHEPSSVGTILFTLTPCVLFLGLIQTLTGIIQGM
                     GKPIVPVIALCVGMLCKIVISYTLTGIPDINVLGSAFGTVTAYFVAAMINLLYVKKHM
                     NVNFSKKEFIIKPFITVMTMFIMVKLSYGALVGFLGNSISTIIAICVGGIVYVVVILG
                     IGGIKKEEILTMPKGDKLYKLLKKVKLIR"
     gene            58183..59631
                     /gene="mazG"
                     /locus_tag="ACGI59_000110"
     CDS             58183..59631
                     /gene="mazG"
                     /locus_tag="ACGI59_000110"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HFG9737924.1"
                     /translation="MGKISIVGLGPGDYSLISQGVLESLINSPKVFFRTEKHPIIDKL
                     KETIQYTSLDYFYEKEENFDNVYLKISEFIIEESAKGDLVYAVPGHPRVAEKTVSIIE
                     RLANEKHIAVEILPSMSFVDAMFNYLAIDPSEGFKLVDAFEIENSYIDLDTSMIITQV
                     YDKFIASNVKLKLMEHYDYNQEVCIVNGAGVKDLESKKFIKLCELDRNENFFDYLTSL
                     YIPKSSKKMYNTVHDLEIIVNTLRSPSGCEWDKKQTHQSLKNSVIEEAYELCNAIDNN
                     DIDEMVEELGDVLLQVIFHCQIGNEEGYFDLKEVVNGICKKLIHRHPHIFSGAKLDMS
                     KFEKTWEELKKEEKGESTITDGLRRIPKHLPALMKASKVQHKAALVGFDWDNVEDVFK
                     KIEEEYQELLDEHRVGNIKYIKEELGDLLFSIVNLARFLDIDSGEALNCTTEKFINRF
                     DFIEKSAMNLNKKLEDMTLEEMDEFWNQAKKQ"
     gene            59720..59998
                     /locus_tag="ACGI59_000111"
     CDS             59720..59998
                     /locus_tag="ACGI59_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HFG9737925.1"
                     /translation="MNKAELVSKMAEKSGLTKKEAEAALNAFMSSVQDALVNNEKVQL
                     VGFGTFETRERAARQGRNPRDPEQVIDIPASKAPVFKAGKGLKDIING"
     gene            60070..60312
                     /locus_tag="ACGI59_000112"
     CDS             60070..60312
                     /locus_tag="ACGI59_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFG9737926.1"
                     /translation="MRLDKFLKVSRIIKRRTVAKDACDKGIVTINGKVAKSSSEVNIG
                     DTLEIQFGEKKMKFKINEVKEHVLKNDAKEMYEILE"
     gene            60374..60628
                     /locus_tag="ACGI59_000113"
     CDS             60374..60628
                     /locus_tag="ACGI59_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YabP/YqfC family sporulation protein"
                     /protein_id="HFG9737927.1"
                     /translation="MEQNITLKDRSKLVISGVEHIYSFNDKRVELKTSVGEMVIEGEN
                     LDMSKLSIDENIISIDGTINSMVYAKPKKPQESFLKKVFR"
     gene            60629..61162
                     /gene="yabQ"
                     /locus_tag="ACGI59_000114"
     CDS             60629..61162
                     /gene="yabQ"
                     /locus_tag="ACGI59_000114"
                     /inference="COORDINATES: protein motif:HMM:TIGR02893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0034301 - endospore formation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex biosynthesis protein YabQ"
                     /protein_id="HFG9737928.1"
                     /translation="MIPFTQDVSIFYATIYGGILIGVLFDFYRGLRGNFKFINYFAII
                     FDVLFWFLATVIIFVTINLTEFFDLRYYHFVALFIGFILYYNTISKIVLSIINKIIRF
                     VRNSFKKVTHYIVSFLNNLYYVIIYSLHLLFDIIFYIPNIFIATRKSIKRKSNKKLKN
                     KKKSKPKKKKNKTKKRV"
     gene            61168..61452
                     /locus_tag="ACGI59_000115"
     CDS             61168..61452
                     /locus_tag="ACGI59_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HFG9737929.1"
                     /translation="MNLRKKFSGQIIVISLFLGISIFSMMTGFVFEYTKAKEYKKEIA
                     SLNKQLKKTEIQINSLKKDEKSYEGDLEDIARKRLNMVKPNETVYVDINR"
     gene            61565..62485
                     /locus_tag="ACGI59_000116"
     CDS             61565..62485
                     /locus_tag="ACGI59_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA family phosphatase"
                     /protein_id="HFG9737930.1"
                     /translation="MKVGAIDIGTNSMRLLIADYRDGNFINRKKFVNTTRIGYGVDSK
                     GYIKEDAIKRNIKALKEFSDKCNDEKCEKIYCMGTSALRDSKNGDYFVKLAKEETGID
                     VDIVTGIEESNLGFKGVLEGIQGDDTIIVIDIGGGSTEFIVGDSEGIIFNKSENVGAL
                     RMTERFLKHDIIEDEEFEAMSSFIYGEIDKTLNHIKDKGLKTLIGIGGTITSLSAMNQ
                     ELEIYSMEKIHNSKIHKKEIEEILQKLIKMTLSDKKNLKGLQEKRADIITAGVKILNI
                     IMEKLEIEEITVSEYDNLEGLICQKSKKMY"
     gene            62575..64947
                     /gene="spoIIE"
                     /locus_tag="ACGI59_000117"
     CDS             62575..64947
                     /gene="spoIIE"
                     /locus_tag="ACGI59_000117"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein E"
                     /protein_id="HFG9737931.1"
                     /translation="MQRSVAVVKNKTVKPNVIKLISGYIDFTTAAFAVMGFLLSRSII
                     VDSVAPLGIAFFICASKIDKYKIPVFISTLLGILLSSNDFGNVAKYAVCLTIFMAMNK
                     KLKLSDSIAKVAFIGAIILLPISMGQAFFSNRYVYGLLIAGVEAIIMFISIYVFSYGI
                     GLMANVNNRMSVRTEEAISISLLLTFSIMGIGDISLFGISIRTILSTVLILLTSIVGG
                     ATMGASSGVIIGVASILNNMTSAVYMGIYSFSGLISGAFNKINKYFCILGYILSWTII
                     YLYTSGITSNMMQLRDILLGCLIVLVLPERLFNKIEKLIKSNVASNEIVYDYIMRSKN
                     LTNSRLNSIYKTYDDLADTFDKIREKDKVLDQRDIANVIDMIHNDECKSCSMRRMCWE
                     SRFNHTYTMIYNILEKIEEKGELSLNDIPKNFRKECMKPESIVKISNHYYKMFVLDYD
                     WSVKFSESRKLIANQIRSISKSIKSLSQDLEGDIMLDIEKEKNIYEQLERYDITVDKV
                     SYLTKSNSEFEISIEKKTCHDGCMCEDKIVNIISDLVGENMSVRKIGCHCLGGKCKAT
                     FVKSQKYKAVTEVSAMSRDGHILCGDNYTYMEINDGKYMMAISDGMGKGKKAYEESSA
                     TIDILEKMIDAKIKDEIVIDTINNMLLLKSSEEMFSTLDLGILDLKRGCLETIKMGAC
                     STYIKREDGEVDLISSSSLPVGILSDVKIDRKNVKVKEGDYVIMVSDGIVDAGRNNNL
                     GDNWLIYFLKNIETTNPKEISNLILDRALELQALQIEDDMTVLVTKICTK"
     gene            65298..67190
                     /gene="pepF"
                     /locus_tag="ACGI59_000118"
     CDS             65298..67190
                     /gene="pepF"
                     /locus_tag="ACGI59_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HFG9737932.1"
                     /translation="MNRKLCKAIIPLFISIFCMLIISIKADQGYQKNNDRAITVMNNN
                     DTEDDAEKKQEIDYEKTCWNLQSLFKSDDQWKKELKSFDKDTKELKNYIGKVTKSPTH
                     LSFALNIKEKLDIRLNKLSAYVKLKQDTNKNSYKYLDMNDSMSKSYGNYMGICSDLEL
                     EILKLSNKDYKKIISNKNINRKYGAYLRDIRRNKVHYLDDKSEDILSKVSSISSLPSQ
                     VYELFRNMDRKTNLTPAQYASELESSDRENRKKAYQDELITYNDNINTIAGLITGQVK
                     KNIFYSQERGYESSLEMYLESDNVDEKVYNNLIRTVNSNMDSLHKYIDLRKKVLKLDK
                     VHSYDMFVPMVKAVDNNINYEKAQSIVYSALSPLGKEYNDVVYKAFNEKWIDVYSHEN
                     KVSGGYCLSVYENHPYILLNYNNSLGSVSTLSHELGHAVYEYLSSKNQNYFNSNPSIF
                     THEVASTTNEALLYEMLIKEAKGNDEKAYYITQYLDLIKDTLYTQTMYAEFEKTIHEL
                     VEKGKSVNALVLNDVWGQLLKKYYGQSYELDQLSKVGWARIPHFYNSFYVYKYATGCS
                     AGVSFAQDILKNGSDNYMSFLKKGSSDYPINLLKSSGINLTNTKPIENTIKKFDSLVA
                     ELEKLL"
     gene            67304..68269
                     /gene="galU"
                     /locus_tag="ACGI59_000119"
     CDS             67304..68269
                     /gene="galU"
                     /locus_tag="ACGI59_000119"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFG9737933.1"
                     /translation="MQVKVKKAVIPAAGLGTRFLPATKAQPKEMLPIVDKPTLQYIIE
                     EAVASGIEEILIITGRNKKSIEDHFDKSVELELDLEKKGKKELLEVVQNISNMINIHY
                     IRQKEPKGLGDAIYCARHFIGDEPFAVMLGDDIVDNDVPCLKQLTDAYEEYRTTILGV
                     QKVNQEDTNKYGIIEAKNIEGRVYKVKDMVEKPESGKAPSNIAILGRYIITPEIFDIL
                     KDLPPGKGGEVQLTDALKILSKKEAMYAYNFEGKRYDVGDKLGFLEATVDFALKKEDL
                     KEDFIKYLKHVCSEFDKNNNVLYNNTDEKIDLIEVNEARELEIQK"
BASE COUNT        27665 a         7333 c        12384 g        21079 t
ORIGIN      
        1 tattaaaaac ttaatagaaa tattaagcat aaagattatg tggttacaat agcagagagg
       61 atacacctgt tcccattccg aacacagaag ttaagctctc tagcgctgat ggtacttggt
      121 gggaaactgc ctgggagagt aggacgtagc cacgtaattt tttttatttt ttgtcaaaaa
      181 aaacaaaaaa tacgtatttc ttttttcatt tcgggaaaac taaacacagg aattatggac
      241 tttataaaaa tttaattctt gaggagtgat ataagtgaaa aaagttgctt taattttagc
      301 tataataggt gcgcttaact ggggtgcaat tggtattcta ggtacagact taataggtag
      361 tatctttggt ggaacatatg aaatggtaag tagaataata tacttcattg taggtttagc
      421 tggattatac ttaataccaa gtttaatgtc tgatgataga gaataattta tgcttttatg
      481 ttaaatacct cattctaaat gtttagaatg aggtatttaa ttttataaaa atattaattt
      541 gtttcttttt taatggtaaa atagataaga attgaagttt taaaaagaag tattatgtaa
      601 aaaaggagta ctattaatat atgtttatta ttaatgaatt aaataagatt gtaaatgaag
      661 aagtaatatc ttttcatatg cctggtcata aaaagggaca ggtttataaa aagttaggat
      721 ataaagatat actaaaaaat ttgtataaaa tggatacaac tgagatatta ggaacagata
      781 atttacactt tccggaagga attataaaaa attcacaaaa aaaagcatca agagtattta
      841 aaagtgatta tacttgctat ttagtaaatg gtagtagctg tggaatacaa tctgcaataa
      901 tgtcagtatg tagtcctaaa agtaaaataa taacaaatag agactgtcat cagtctgtta
      961 taaatggatg tattttagga gatatagata tagagtatgt gccttgtgag ataagtgaag
     1021 agacaaatgt cttaaaaggg atatctataa gtaatgtgat tgatataata gataaaaact
     1081 tggatgcaaa agcagtactg cttacttatc ctacatatta tggaatgaca tatgatttag
     1141 aatacatatg taattatgca catagcaaaa agatggtagt gatagtagat gaagcacatg
     1201 gtgcgcattt agggcttagc gagaggcttc ctaaaacagc tcttgagcaa ggcgcagata
     1261 tagttgtaca gagtacacat aaaactcttc cagcatttac acaatcgtca atgatacata
     1321 tacagggaga ccgagttgat atagataaaa tattgaacat gttaagaatt acagaatctt
     1381 caagcccttc atatttatta ttatcttctc tggaattagc agtagatata tatgaaaata
     1441 aaggaaaaga attaatggaa gagcttttaa ataatattat aactttcaaa aataaagttg
     1501 attcaaatat taatatatac gacacaaatg ataaaacaaa aatatttata tcatctaaaa
     1561 atatgggttt aacaggttat gaactagaaa atattttaag aaacaaatat aacatacaag
     1621 tggagttatc taattattat ggagttcttt taatatgtac aataggaaat actgttgagg
     1681 atttctctag tttagaaaca gcattaaaca atattgtaaa ggaagacttt aaatttgaaa
     1741 agttagaaaa taaaaaatac cctgttgtta ttccaaagaa aatattaaca cctagagaag
     1801 cattttataa aactaaaaaa agtgttaaaa tatatgacag cataggtaaa gtttgtggtg
     1861 aatgtatagt tccataccca cctggaataa gtgtaatatc tcctggtgaa gtaataagta
     1921 aagaaataat agactatcta atgttttgtc attctaaagg catgattgtg agtggattga
     1981 aagatgtaga tttaaatttt atacaaataa tagatttaga agacatttaa aaattatgat
     2041 ataaaagtag atagaagtag atagtttaaa attgtgaaga gaaataaata gactgcttat
     2101 atagagctat ctaataaaag tactaaaaaa gtaaaaagtg tttgtatgga ggatatgaaa
     2161 atgaaaggta agttaataat tatagagagt ggttcagatg caagtggaaa ggctactcag
     2221 acagaaatat tatatgaaag attaaaaaaa gaaggctata tgattaaaaa ggtagaatat
     2281 ccaaattatg aatctccatc atctgccttg gtgaaaatgt acctaaaagg agactttggt
     2341 aaaaatgcat cagatgtaga cccatatata gcatcaactt tttttacagc tgataggtat
     2401 gcatcattta aaaccgattg ggaggagttt tacaatgaag gtggaataat tatcgcagat
     2461 agatatacaa cctctaatat ggtacatcag gcctctaaaa tggatatttg tgatagagaa
     2521 gagtatttaa attggttagc tgattatgaa tttaatttat ttaagatacc acaacctgat
     2581 tgtgttatat ttttagatgt gcctattgaa tttagtaaaa agcttatgga gaatagaaaa
     2641 aataaaatta caggggaaga aaaaaaggat attcatgaaa gtgacataga gtatttagag
     2701 aaatcttaca acaatgcttt atatatagca gataaatatg attggaaaaa aataaattgt
     2761 gttttagata acaaattaag aagtattgaa agtatacata atgaaattta taaaatagtt
     2821 cttgattcaa taaaacctat ggagagataa atatgtattt tgaaaatatt atagggcaag
     2881 attttgcaaa gaaatattta acaaattcta taaagaaaaa taaacttaat aatgcgtata
     2941 tgtttgaagg aatggatgga ataggaaaaa agaaatttgc agatgaatta tcaaaactat
     3001 tacttgatta tgaaaatctc gaaaatagtc cagactatgt actcataaaa ccagatggaa
     3061 atagtataaa aatagctcaa atcagaaatt tacaatcaga tatagtaata agacctcata
     3121 aagactataa aatatatata atcaataatg ctgaaaaaat gactgtggaa gctcaaaatg
     3181 cacttttaaa gacgctagag gaacctccaa actatgctat aataatctta gtcacaaaca
     3241 acaaggaatc tttattagaa actataaaat ctagatgtga tataataaag ttctcaccta
     3301 taccaattga agacttaaaa cgttatttga taaatacagg tatagaagaa gaaagagctc
     3361 aattattggc tattttttcg agaggaagta ttgagaatgc tctaaatcta tctcagtctt
     3421 ctgagttttc aatgatgaga gaagatatac aacagtatat tcagataatg ttagataaaa
     3481 acatagtgga gattttaaat ataccaaata atatggaaaa atatagaggt aagattatag
     3541 ctttattaga tatgatgata aactattttc gagatatcat cctattaaaa gaaaatgtaa
     3601 acaagaacat gttaataaat gtagataagc tagttttcat tcaaaatatg agtggaaaaa
     3661 ttagttattc tcaattgtcc aagattattg atataataga agatgcaaag agtaaaataa
     3721 aaagcaattg caatttcaat ataagcatac aagttatgtc tttaaatata tacgaggtga
     3781 ttaaatgata aagatagtag gtataaggtt taaaagtgct gggaaaatat attattttga
     3841 ccctgttgat tttaatatag agcaagatat ggatgtagtt gtagaaactg caagaggctt
     3901 ggagtatggg aaagtagtcg ttggtccaaa agatatggat gaaagcgaat tagtttctcc
     3961 tttgaagcca attataagaa tagctacaga agaagataaa aaagtatatc tagaaaataa
     4021 ggaaaaggca aaagaaactt ttgagttgtg ccaacaaaaa ataaaagaac atgagttaac
     4081 aatgttttta atagattgtg agtatacatt tgataggaat aagttgattt tttactttac
     4141 agcagaagga agaatagatt ttagagaatt ggtaaaagac ttagctgcta tatttaaaac
     4201 tagaatagaa cttagacaaa taggtgtcag agatgaagct aaatctatag gaggtctagg
     4261 gccttgtggt agaaaacttt gttgttcatc ttggttaggt gattttcaac cagtttcaat
     4321 aaaaatggca aaagaccaaa gtctatctct aaatccaaca aaaatatctg gaatatgtgg
     4381 gagattattc tgttgcttaa aatatgagca tgatgtatat agtgaagcaa tagaaaaaat
     4441 gcctgttgta ggtgcattgg tgcaatcaga tgatggaaaa ggaaaagttg tagaagtgaa
     4501 tcctttatta gaacaaatta aagtggaatt tcaagacaag acagtaaaga tatacacaag
     4561 agaagatata aaaatactta gagagcctaa aaaatgtgat ggctgtggtg gtaaatgtaa
     4621 ggataatgat ggattagatg aagccacact aagagagcta aagaaattag aagattaaat
     4681 ttaaggtagg gcttttccta ccttttttaa agcaattaat gcttaaagtt attaatattt
     4741 gaggagaatt tatatatgca attaaaagat tcagaaagaa tagatgattt acaattaaaa
     4801 ggccttaaaa ttatacaaga tactaatggt ttttgttttg gtatagatgc agttttatta
     4861 gccaatttta ctaaaataaa aaaagatgcc aaagtagtag atttgggtac tggaactgga
     4921 attataccta ttttgatagc tggaaaaagt gaagctaaaa aaattattgg cgtggaaata
     4981 caagaagatg tatatgaaat ggcaactcgt tctataaaat taaatggatt agaagaaaga
     5041 gttgagatta taaatgaaga tataaaatac atagacaaag tcttagacat aaatggttac
     5101 catgtagtca catcaaatcc tccatatatg catattgatg gaataaaaaa tccaaatgat
     5161 aaaaaagcaa tatcaagaca tgaagtaagg tgcaatttgg aagatgtaat tagagctgca
     5221 tccagattgg ttatgcctag aggaaaattc ttcatgatac atagaccaac aagattggtt
     5281 gatataataa ctttagggag aaaatataaa ttagaaccaa aggttattca atttgtgcat
     5341 ccaagacctc aaaaagcacc gaacttagtt ttggttcaat ttgtaaaaga tggaaggcca
     5401 gagcttaaaa tattggaccc attgtatgtt tatggagaag atgggaatta tacaaaagaa
     5461 ttaaaagcaa tatataataa taaagatata ggagagttgt aaatgagtgg gaaattgtac
     5521 atatgtccaa caccaatagg aaacctagaa gatataacat atagaacttt aagaacatta
     5581 aaagaagtag acttgattgc agcagaagat acaagacata gtataaaact tttaaatcat
     5641 tttgaaatct caaaaccact aactagttat catgaacata ataaggattc aaagggagat
     5701 tatttgataa ataaactaat tgatggtgaa aatatagctc tgataagtga tgcaggtatg
     5761 cctggtatat ctgacccagg agaagagata ataaaacaag ctatacaaaa taacatagag
     5821 atagaagtat tgccaggtgc aacagccttt gtaacggctt tagttggctc tggtatggac
     5881 acacatagat ttgtttttga gggcttttta gatagagata aaaaagttag gaaagcacaa
     5941 ttggaagaat taaaggaaga aagtagaact ataattttct atgaatcacc acatagattg
     6001 aaggatacac taaaagatat gcttaaaatt cttggtaata ggaatatttc tataaataga
     6061 gaacttacaa aaaaatatca agaaataata agagaaaata tagaaactac cataaaaata
     6121 tttgaggaaa aagaaataag aggagaattt gtattaatag tagagggttt tcatggtgag
     6181 aagtcaaaga aagaaaatta tgatgggctt acagataggg aatatgtttt aaaactaatt
     6241 gaaagtggta tgaacaaaaa agatgcaata aaaatagttt gtaaagatag aaagttaaag
     6301 aaagatgtgg tttataaaca agttttagat ttataaaacc tgaatctgag gtgattttat
     6361 ggaaataggg aaaattataa tagagagttt aaagagggaa gatacacata aaatatttat
     6421 agaattagat aataagcatg aattagaaaa aataattcct aaaataagct gtatgaagtc
     6481 tgtaggtgaa tgtaaatatc atgtagtaaa ttgttttgaa cattctataa acgcattaaa
     6541 agaattagaa atagtattaa atgataagga tttttttcca atacatctaa gaaaacatgt
     6601 gtcaaactat ttaaacacat atattgaaga aggaataaat aagttacatg tattaaagct
     6661 gggaacattt ttacatgata taggtaagcc tgacagtatg accttagatg aaaccggaag
     6721 agttcatttc acaaatcatg aaaaaatagg tgctcaaata attgacaata tgggaataaa
     6781 gttagagtta tcaacagaga catatagact tataagtaaa tatgtaagat atcatatgat
     6841 acttttgtct ctatataaga aaaatgattt gagtaggaaa gaattaatta atgtatttaa
     6901 cttagtagat gaggacacta taggtgtaat aatgcttggg tatgcagata tagtgtctac
     6961 agtaaaatta ttgggcaaga gtggagaagt cagtgtgtta aagacatata tggaatatat
     7021 tttaacaaat tacttgtata agagtaatta tacacatgta taaatttagt taatataatt
     7081 gagttttaga tgattaattg aagtttgaaa tatacggatt tcaaaaacat aaaagaagtt
     7141 gtctttgttg taaaattatg aaagacaact ttttttatat caaaaattga aattttatta
     7201 aggatactta atggtctatt aattaataaa ccattagtat ttaatttata ttacaatatt
     7261 gattttgctt gtattggcgt tgatagtata acagaggtct tagtagaacc aattttcttt
     7321 atagtatcaa tcacattttc taactcgtac atatctttta caattacttt taaaagtaag
     7381 caatcatcac ctgttatatg gtgacattct acaatcctag ggtcctttgc agctgactca
     7441 ataaattctg tatatccatt gcttggaaga gaaatatgaa taaatgcctt tataactctg
     7501 cctaatgaat ctgggttgac aatagcttta tatccttcta taacaccaga ctcttctaat
     7561 cttttgactc tttctgaaac tgcaggagaa gttaaaccaa ctatttttcc taagtccttc
     7621 atagaaattc tcccatcatc ctgtaaaatt tctatgattc tgtaatctgt aacatccata
     7681 aataaacagt cccccttaaa taaaaataat taatgctata aaactttttt agaatctatc
     7741 atttgtgttt ttaaatacca aagatttcta gataaatcaa ccttataaat gtgtggtttt
     7801 gaaagtcttt tgtattgttc ccagatagta cttaattcat attgtacatg attttttatt
     7861 tcaagaacag cttttttatt atatttgcat ctgcctttta tatacaatgg tttaagaagc
     7921 tctttaactt tgtaatttgt aaataccttc gttttccatg tataagtagg atggaagatt
     7981 tctaaaggtt tagactcatc tataacttca tcatgtaaca taatcaaatc tgcctctgcc
     8041 ttattgtctt cgttatatat tcttataact ttcttgtaac caggattatt tattttttct
     8101 ggattctcag aaatctttat cttaggttca agaactcctt tttcacaaga tgcagctaat
     8161 ttataaactc ctccaagaga aggtgaatcg aaagatgtaa tgagctttgt acctactccc
     8221 catgaattta tagtagctcc ttctgcttta agtgatgtta tggtatattc gtctaggtca
     8281 ttagatgcag tgatgctgat atctgaaaag ccagcattat ctaattgttt tttagcttct
     8341 ttagataaat aagctaaatc tccagagtca agtcttatac ccataggttt gtatcctttt
     8401 tcagaaatat ttttgaatac ttttattgca ttaggaactc cactgtttaa tacatcataa
     8461 gtatctacaa gtagtaggca tttatctgga taaacatcag catatgcttg gaatgcttca
     8521 agttcattat caaatttttg tacccaactg tgagcttgtg taccaattat aggtatatca
     8581 aacattttcc ctgctagtac gtttgcagtt ccagcacaac caccaactac agcagcccta
     8641 gccccatata tccctgcatc tggaccttgt gcacgacgta atccaaattc aaatacaggg
     8701 tctccttgag ctgcaaagca aactctagag gctttagtag caataagaga ctgaaaatta
     8761 actattgtta acaatgctgt ttcaataagt tgagcttgat ataaaggtgc cttaacagta
     8821 attaatggct cattagggaa catgatagtt ccttcctcaa cagcatatat atctcctgtg
     8881 aatttaaatt cttttaaaaa ttttaagaac ttgtcagaaa ataaatttaa gttttttaag
     8941 tactctaaat cttcatctga aaaatgaaga ttatctatgt attcaacaac ctgatcaata
     9001 ccacaaacta tagtataccc tccatcacaa gcgttttttc taaaaaacat atcaaataca
     9061 acaatgtctt catgaatatt cttttcaaaa tagccattta acatagtaag ctgatataaa
     9121 tcagtaagaa gtgttagatt tctcattatt tctctcctta attattatta aatattaaaa
     9181 attgtaaaaa ctcccacact tatactaaaa cataatatag tacaactttc aagtataaag
     9241 tattacctaa ctatcataaa attatttaaa gaggaggatg attataatag gaaaaatatg
     9301 gttttatatg gtatcgatag gaataatagg aagcttgatt tctaaaaata tgggtgaatt
     9361 aaataaagtt atattaagtg aaacatcaaa aggtatagag tttgcaataa gtctagctgg
     9421 tatcatggca ctctggatgg ggataatgaa tatcgcaaaa gaatcaggtt taattgaaaa
     9481 gataggtcaa aaactaaatc ctataatgag aagattattt ccttctattc caaaaggtca
     9541 taaagcaatg tcttatatag ttatgaatat tgctttaaat atgttgggtg ctggaaatgg
     9601 tgcaacagca tttggtttaa aagcaatgag tgagcttcaa actttgaata acaagaaaga
     9661 tacagccact aatgatatga ttatgtttat ggtaatcaat atatcatcta tacaaataat
     9721 tccatttacc ataatcaaac taaggatgga tatgggggct caaaatccaa gtgaaataat
     9781 ttttacgact ttatttgcaa ccataatatc tacagcagta gcaataataa catgtaaatt
     9841 ttttcaaaga agatatagat aaaggggatg tagatacatg gagatatttt caaacttact
     9901 tataccaata ataattctgt acatagtaat ttatggtaaa tataaaaaaa tagatgtgta
     9961 tgatagtttt gtaaaaggag caattgatgg attaaaagct gcatgggata tcctaccata
    10021 tataataggt atatttttgg caataggaat attcaaaact ggtaaaggtc ttgatatgtt
    10081 agaatggcta tttacaccaa tagcaaatat gatgagtata ccaaaagaat taataggtct
    10141 aataagtgta aaacctttat ctggaagtgg tgctttaggg atgtacagtg aacttgcaaa
    10201 tagagttgga ataggaagtt tagtagaaaa aatgggtgct acgattgtag gttcttctga
    10261 gactattttt tacacaatgg caatatacta tggaagtctg aagataaaaa atactagaca
    10321 tacattatca tgtgctatga ttagccatgt tgcaggtgtc atagcagctg tttttatttg
    10381 ctatgtaata tttgtataat ttgttgacaa tatgggaaaa attttataat atagtgatta
    10441 tatagaaaca aatagtaata caaattacaa gctatgaaaa gaagagtaaa taaaggtttt
    10501 ttctatcaga gagctaggtt aggtgaaagc tagtgtcaaa agctttattg aagatggtct
    10561 tggagctttt tatctgagca agtgtgctag ggtaaaacgg ttggacacgt tatagtctac
    10621 taaggtatct ataaaatgtt tctacatttc atgtgaaaca taactattat agatgaatta
    10681 gggtggtacc gtgaataatc tcgcccctat gttaactcat aggaggcgat tttttattat
    10741 ttaaatgaaa gaatcatatg attacgaacg ataaaaaatt aggaggtaaa taacatgagc
    10801 aaaccgagtt tttatgtaac aacaccaata tactatccaa gtggaggatt acacatagga
    10861 catacttatt caacagtagc tgcagataca atagctagat tcaagcgttt ctgtggatat
    10921 gatgtaaagt tcttaacagg aacagatgag catggtgaaa aaattcaaaa gaaagccata
    10981 gaacaaggaa tgtcagagat agagtatctt gatggaatga taaaagatat aaaggcctta
    11041 tggaatacta tggatatatc ttatgatgat tttataagaa ctacagaaaa aagacataca
    11101 gacataatac aaaagatatt tactaaacta tatgagcaag gtgatatata taaaggtgaa
    11161 tatgaaggta gatactgtac tccttgtgag agtttctgga cagaatctca attactagaa
    11221 ggaaataaat gccctgactg tggaagagaa acatatttag taaaagaaga atcttatttc
    11281 tttagattat ctaaatatga agacagatta aaagagttat ttaaagatga tagtttctgt
    11341 tttcctgctg ctagaaaaaa tgaaatggtg gcaaactttt tagacaaagg attagaagat
    11401 ttaagcgtaa ctagaacgac ttttgactgg ggtataaaag ttccttttga tgaaaaacat
    11461 gtaatttatg tgtgggtaga tgctttatgt aactatataa cagcattagg ttatatgact
    11521 gataatgatg aagaatttaa aaaatattgg cctgccaatg ttcaaatagt aggtaaagaa
    11581 atagtgagat tccatacaat aatatggcca gcgcttttaa tggctttagg tctagaagtt
    11641 ccaaaacaag tatttggaca tggatggata ctatttgctg atgataaaat gagtaaatca
    11701 aagggtaatg tggtttatcc agaacctata atagaaagat atggaataga cacacttaag
    11761 tacttcttat taagagagtt tgcatttggg caagatggaa gttatactca tagaaatttt
    11821 gtaacgagaa taaattatga tttagcaaat gatttaggaa acttaataag tagaactgta
    11881 gctatggttg aaaaatacaa taatggtata ataccaacag caaaagtttc aactgatttt
    11941 gatgctgact taaaagaaca agctgtttca actagagaaa actttgaagc tgaaatggat
    12001 aaaatgcaat tccatgaagc attagagagt gtatggaaat tagttagaag aactaacaaa
    12061 tatatagatg aaactatgcc ttgggcacta gctaaagatg aaacgaagaa agatgaatta
    12121 gacacagttt tatataattt atgtgaatct ataagaataa tcgcaacact aataaatcct
    12181 ataatgaatg agactgctaa taaaatatat gaacatatag gaataaaagg tcaagatgat
    12241 ataactacat gggaaagtac aaaaacattt ggtcttattg gagaaaatgt aaaagtattt
    12301 aaaggtgaac ctttattccc aagattagat gtagaaaaag agatagaaga attaactaaa
    12361 atgttctctg ggaaacctcc agtagaagaa aaacctttag agcataaaga agaaattact
    12421 attgatgatt tagacaaaat agagcttaga gtagggaaaa taataagctg tgaaaaacat
    12481 ccaaaagcaa ataaattatt agtatcacaa gttaagattg gaccagaaac aagacaaata
    12541 gtatcaggaa tagctgaata ctataagcca gaggatttag taggaaaaga ggttacagtg
    12601 gtatgtaact taaaaccagt taaattaaga ggtgtagaat ctcaaggtat gatattagca
    12661 gcaggtgatg atggagaccc atatgtttta ccatttacac aaggggctaa agatggatgc
    12721 gaggttcgtt agtaggtaat taaatagtaa ggagattaaa gatgttattt gactcacatg
    12781 cacatttaaa tgatgaaagt tttgatgaag atagagatga acttattggt tcattaaaag
    12841 ataaaggtgt agatttggtt gtaaatccag gagcagatat agagacgtcg ataacagcaa
    12901 ttgaacttgc gaaaaaatac gactttatat attcagcagt tggggtacat ccacatgatg
    12961 tatcaaaact agatgataca gcaatagaaa ctctaagaaa acttgcaact gagaatgaaa
    13021 aagttgtagc tattggagag attggattag attattatta tgattattct ccaagagaag
    13081 aacaaaaaga gtggtttaag aagcaaattg aattagctaa tgagctaaaa cttccaataa
    13141 taatacatga tagagatgca catggagata cttttgagat aataaagaat actaaaaatc
    13201 ctgaaatagg atgtgtactt cattgttata gtgggaatgt agagttagct agagaatatg
    13261 ttaagatggg ttgttatata tcaattccag gcacggttac ttttaagaat aataaaaaaa
    13321 ctagagaagt agtaagagaa atacctctag aaagattatt catagagact gattcaccat
    13381 atatgtcacc agagccacat agaggaaaga gaaacaatcc ttcccaagta tcttttgttg
    13441 ctgataagat agcccaggaa aaagggatat cttatgaaga agtatgtaga gttacaaaag
    13501 aaaatgcaaa gaaatttttt aatataaaat agacaaatta aatcttataa tgatatatat
    13561 aataagtcat tataagtata ggatgagagt gcctcaaaat gagtttttta gttcatgagg
    13621 cactcttttt tgtatgaaat caaatatatt ttcatcattt caacaatgaa aaagaagttt
    13681 atcatatttt gagatagttt gtttttattt atttaattca tattttaaaa tatattttct
    13741 taacattgtt aaaaaagaat ttacaagaga ttcattagaa ttaactcaac tctaatattc
    13801 acttaactta agttgtttat tatattatta aagagaagta aacgaaaggt agattaagga
    13861 ggttgaattt tgtatacagt tgatagtact aagatattga taaatatgaa cttagagaaa
    13921 ctagaaaaga tatactcaat gataaaagat acatcaagta tttctataat taaagaacct
    13981 aacttggcta cagtaatggt aagagctaat gaaagtgtaa aaaacacaac atttaattta
    14041 ggagagatat tagttactga atgtagtgta aaggtagatg aaagcttggg ttacggaata
    14101 gtttcagaaa acaataataa gaaagccatt tatttagcag ttatagatgc agttttacat
    14161 agcaataaca gtaaatttga tgagttaaag aattacataa ataaaagtgt atatgaagag
    14221 aacttaaggt atgaacaaga gattattgat gaatttagct tgattaataa aacgaaagtt
    14281 caattcaatg ctatggatta aataggagga gaatcatggt aaattataca caagaaatat
    14341 atagaaagat gctagatagt gcttcatttc ctgggaaatt aaataatata gataatataa
    14401 atatagaaaa taatacaaat ctattgaatg gagcaattgg tatagcaatc actttattag
    14461 accaagaagt taccttctat atagaaaatt atactcaaaa agatataaaa aatataagag
    14521 cacttacaat gtcaaatcaa atagattata gaaaatcaga ttatatattt ttagatatta
    14581 attcagaagt ggatatatta cagattaaaa taggttcttt tgaatatcct gatgaaagtg
    14641 caactatagt acatcaagta aatgatttat caacgtactc tcaaactaag tatataaagc
    14701 ttgatttgag tggtcctggt ataaaatcta aaaatagtat atatataaat ggaattagag
    14761 aagaatttat agaaagatta tctagtataa ataaagatta tccaatagga atagatttga
    14821 ttttagtaga taaaaaagga gaaattgcat ttataccaag gtcatcaaaa ttaagttggg
    14881 aggtattata gaaatggctt atgtagcagt taaaggtgga gaagaagcca tattacaggc
    14941 taaaaatata cttgagtttt atagggtaaa aggagatagc ttgcctatag atgtaaagca
    15001 gattgaggaa caaatgtctt atgctgtaga caaagtaatg tctgaaggaa gtttatacaa
    15061 caaggaacta tcagcattgg ctataaaaca agctcaaggt gatatattag aagcctcttt
    15121 ctatgtaaga gctttaaggt caacaatgcc tagaattaca tattcagaac ccataaatac
    15181 agaagaaatg agaataaaaa gaagaatatc agctactttt aaagatatac caggaggtca
    15241 gtatcttggg gcaactagag actatgaaaa tagaatattg aatgtagaat tattagaaga
    15301 agatgaaaaa aagaatagac attttagaga tagttttata aatgaattag aaacagtaga
    15361 tgaaatccct gaatttacaa aaatatctaa tatatttaga aaagaaaatt tattacaaga
    15421 agaagtttgt gaaaacaaat tggaagaagt tattgatgat attacaagaa atagtttaag
    15481 atttccagtt tctagggcag caaagttaca ggcattaact agaggtgaaa gcggagtagt
    15541 atcaggtttt gcttatgcta ttgtaagagg ctttggtgat gaacatccat atataagtga
    15601 acttagaact ggcaatgtgt ttataaaaat aaaacatccc atctatgaag atgaagaaat
    15661 aacaataggt gatatgttgg ttactgaatg tgaaatagtt tcaaaagcat cgcaaaataa
    15721 gactgaaaat tctaaaaata attcggaaga taaaaataaa atgtctttag gatatggtct
    15781 ttgtatgggg aacaatgaga ataaggctat atctatggct atactagaca aatctttgga
    15841 gccaaatagt gaaaaactag cacaagacga agaatttgta ttgcttcata tagatggaat
    15901 agattcatta ggatttatat ctcactttaa gcttcctcac tatgtagatt ttaaagcgga
    15961 tgtagaaaga ataaaaaagt ttgaggaggt aagtttaggt gatatataac tatggttttt
    16021 tagacgaaaa cacaaaaaaa gaaataagaa gaaagatatt aaaagcaata tcaatacctg
    16081 gttatcaagt tccctttggt tcaagagagt tgcctatagc taagggatgg ggtactggag
    16141 gtttacaatt aacactatca ttaattggta aaaatgatgt agtaaaagta attgaccaag
    16201 gaagtgatga ttctacaaat gcttgtaata taaggagctt tattgcaagt gtaagtaatg
    16261 tagaaaccac aaaagataca ttagaagcta cattgattca aacgagacat agaataccag
    16321 aagaaaaact taaatctaat caaatactaa tatttcaagt accaatacca gaaaccctta
    16381 gaatagtaga acctagtgaa gtagaaacaa gaagaatgca ttcagaagaa gattatagta
    16441 ggatgtgggt atatctttat gaagatatag ttagatttaa tgatatatct atagcagttg
    16501 aatatccatg taaagtaaat gatagatatt taatgaatcc atcacctata cctagatttg
    16561 atataaaaaa attaaatatg tcagacaatt tattcttgtt tggtgcaggt agagaaaaaa
    16621 gaatttatgc tattcctcca tatacaaagg tagaaccttt agagtttgag gattataagt
    16681 ttgaagagga aaagtttgaa ggaaaatact gtagtttatg taacagtaca aatacatttt
    16741 tagatgaagt atatgacagt gacactaatg aaaagtacta tagttgctca gatacaagtt
    16801 attgtgaaaa agttagatta aaaaataaca gtatagatgt gacaatagga ggtacttgga
    16861 atgagtaaaa taaatgactc taaaagatta gaaaatatag acaagcaagt attaatagga
    16921 agaaatatca atcttattta tggagatggg tgtgaaaagt gttttgaaag cacaggatat
    16981 gaaagcaata atatatgtaa gtattgtggt tctatagttg catgtaataa tgtaaattta
    17041 gaactttatg aaggagaagt ccttggaatt gtaggtgaat ctggttcagg aaaaagcact
    17101 ttattaaaaa tactgttttt ccaagaacat gcaaatagtg gagaagtata tatatcccat
    17161 tacaataaag acacaaatat actagagctt tcagaccaaa aaaagagata cataaagaat
    17221 cattttatgg gtattgtgta tcaaaatccc caccttggtt taaatttaga ctttagtagt
    17281 ggtggtaata tagcagaaaa attattgatg gcaaatttat ataatgttga aaaaataaga
    17341 gatagaggca aagaactttt aataaaaact aatatacctg tagaaagaat agaccataaa
    17401 ccaaaatact ttagtggggg aatgcaacaa cgagttcaaa tagcaaaagc tctttcaaat
    17461 aaccctccaa tattgctatt agatgaggtt acaacaggtt tagatgtttc tgtgcaagcg
    17521 gaagtattgg atttaataag agaaatccaa agagaattta agatttctat gatagttgta
    17581 tcccatgact ttgatgttat aaagatgctt gctgatagga caattgtaat gaaaaatgga
    17641 agtgtaattg agtctggtct tacagaccaa ataatggagg acccacaaca cccatatact
    17701 cagcaactta taaattcatt gctataaata tcgtttgtaa ataagcaaaa tgggggtttt
    17761 taataaaatg aaagaaacta tacttgaagt aaataatttt agtaaagact ttgagttaca
    17821 tgcattgaac aagactataa cggcatgtag taatataagt tttgaagttt caaaaggtga
    17881 gtttttagga ataataggta aatctggagc aggtaagtct accatattaa aaagtatcta
    17941 taaaacatat ataccaacaa ctggagaaat aatttttaat tcagaaatat atggtaatgt
    18001 agacctggct aaaattggag accgagaaat gataaatctt agaaagaaag aaataggata
    18061 tgtatcacag tttttaaaga cacttccacg aataactgca attgaactgg tagtacattc
    18121 tttaattgag agtgggtttg aaaaggattg ttcgtatgat atggcaaaag atatacttaa
    18181 ccaatttgaa ataaaggaaa atttatggga tgcgtatcca aataattttt ctggtggaga
    18241 aaaattaaga ttaaatttag ctcaagctat ggttaaaaag cctagacttt tgttacttga
    18301 tgaaccaact gcatcacttg ataatcaatc taaaatatat gtaaaagaaa aaatgttgga
    18361 gcttaaaagt caaggaacta caatgatagg aatttttcat gatatagagt ttatggaaac
    18421 tgttatagat aagaccttta ctatgacaaa agggactata agtgaaagtg gggtagccta
    18481 gatgataaaa ctaattcata atgcaaatat tgtattagaa gatatgattt tagaaaatgg
    18541 tcatttaatc atagagggag atacaataaa aaaagtatca aatgataaga ttgattacgc
    18601 atttgacttt aatgagatag atgaaataat agatgcaaaa aacttatatg taatacctgg
    18661 tattatagat attcacagtg atgccattga aaaggaaata gagccgagac caagtacatt
    18721 attaccattt aatatggcat tttatgaact agataaaaaa ctacctgcaa atggaataac
    18781 tacagtttat cattctatat ctttagggga tggagtagga gttagaagta ttgataactc
    18841 tttaaagatg attaaaaata ttgattcata taaaaatata gatagtaaga gcataaatca
    18901 taaagttcat ttaagatatg aagtgttgta ttatgaaggt ttagaaaagg ttttagaact
    18961 attagatgaa aataaaatag actacttatc aataatggac cattctccag gacaaggtca
    19021 gtatacaaat ccaacatttt ataaagagta tgctactaaa gtgtggggag ttacagaaaa
    19081 ttatgtagat acatggttag atgatttagt aaatctgcat gataatttag attggaataa
    19141 aatagctaat attataggta tagccaaaac taaaaatata aatgttgcat cacatgatga
    19201 tgatacatta gaaaaaatgg atttcataaa aaatataggc ataaatgtat ctgaatttcc
    19261 tataactctt gaagttgcaa aacattcaaa gaaattgggt gttctaactt gtttaggtgc
    19321 accgaatatt gtaagaggta aatctcataa taacaattta aaagcaatgg atgctatatt
    19381 ggaagattgt tgtgacattg tgtgttcgga ttatcttcca tcagctatga taaagtctat
    19441 gtgtatagtt gcagaaagaa taaataattt aaataaagca gtcagtttat ttacctcaaa
    19501 tcctgctaaa gcagttggta tatatgatga aagaggtagt ataaaagaaa ataaaaaagc
    19561 agatttagtt ttagtagata ttgatagtga atatccaaag gttgtaaata ctattgtaaa
    19621 tggaaaaaca gtttataaaa gggaggtata aagattgcta acagagttac attgtcatac
    19681 aagtatatca gataataatt ttactactga agaaataata aaaaaagcag cagaaaagaa
    19741 tataaatatc ttagcaataa caaatcatga cacattatct ggattggatg aagctcttgt
    19801 gcttggagag cattatggaa taacaataat accaggtata gaaatttcag cctatgatta
    19861 taaaaataga aaaagagttc atattcttgg atataacata gatttaaaca gtcaagaaat
    19921 aaaaaatttg tgcaatccaa tggtatgtga cagacataaa gcatctgtta agatggtaaa
    19981 taaaattata gatttaggat acaaaatatc tttggaagat gtaaagaaat attcatcaaa
    20041 gactggaata tttaaacaac atataatgaa agctcttata gataaaggat atacagataa
    20101 tatatattca agtctatata aagaattgtt tcatagagga aacggcaagg tatatgtatc
    20161 tttaaaatat gtggattaca gagatgcaat aaaagctata aaaaattctg gtggaatttg
    20221 tgtgttagct cacccgggtc aaatggataa ttttagcgct attgaagaga tggtaaaagt
    20281 aggattagat ggaatagaag tatatcatcc atctcacaac aaagaactag aaaaagagtc
    20341 gctttcttat gctaagaagt ataatctagt ggtcacagga ggctcagatt accacggatt
    20401 ttattctcaa cataattatg aacttggaag caagagctta aataaagaag atttatttaa
    20461 gtttaggtta gctttaggag gggtattata aatgtctttt ttacaagtaa aaaatgtagg
    20521 taaaagttat ggacaagtta aggtgttaaa agacatatct attgatattg aaaaaggaga
    20581 gtttatttgt ttactaggtc caagtggttg tggaaaaagt acactgctta gaataattgc
    20641 agggcttgag gataaacatg gtggaaaaat aataataaat gataaggata tgactaattc
    20701 accacctgaa agtagaaatt ttggtatagt ttttcaatca tatgcattat ttccaaatat
    20761 gaatgtttac aaaaacatag cctttggact agaaaataaa aacataagta aatctaatat
    20821 tgataaaaaa gttaaagaag ttctagaagt tgtagaatta agtggatatg agaaaaaata
    20881 tccatcacaa ttatcaggtg gacaacaaca aagggttgca ttagctcgtg cgattgcttt
    20941 agagccagac tttttacttc ttgatgaacc tttatctgca ttagatgcaa aagtaagact
    21001 taaacttagg gagcagatta gaagtttgca tagaaaacta ggcattacaa ctataatggt
    21061 aacgcatgac caagaagaag ctctttgttt agctgataag atggttgtta tgaatagagg
    21121 agaaataata caagttggaa cgccaaagga ggtatataaa aatccagaaa caccttttgt
    21181 agctgatttt ataggaacaa taaattttat agatgatggt attaataaga tagctattag
    21241 accagaagat ataaaagtag agtctaatag agattctaaa gataaggata taaaagtggg
    21301 agaaatttta gatatcgaat ttagaggatt taactataga attacagttg agtatcgttc
    21361 taagcagatg aaattagatg tagtttccaa agttgcagaa caaatgaaac tgtgtatagg
    21421 ttctaaaata aactttaaga tacctaaaga aggaattgta caatataaat cagagggatg
    21481 tgcttaatag ctaggggtga ataaatgata acatcaaaaa ataaaagaag aataagtgat
    21541 agcacaatac aaaaatattt cttgattttt tcggtaattg tactaatagt atttatatta
    21601 tttccattag tatcattatt aaaaaatgca tttatggata caagtggaaa ttttatagga
    21661 atttcaaact ttagtaaata tgttgaaaat ccaagtctta tagcatcatt taaaaattca
    21721 atttttgtat ctacagtctc atctttaata tcactatgtt tagcatttat atattcatac
    21781 gcaataacaa gaacaaacat aaagtttaaa gaatttttta aaactatggg aatgtttcca
    21841 ctttttgcac caactatgtt atatggtatt tcacttatat acctttttgg aaataaggga
    21901 atctttacaa ccatgggtct ggaagtacca ctatatggtc cattaggaat aattatttct
    21961 cagattctat ttacatttcc acaagctttt ttaatattat ctgtagcact ttcgatggct
    22021 gattacagac tttatgaagc agcagatagc ttgggtgcta gtgaatttaa gaaattttgc
    22081 aaaataacaa ttcctggtat aaagtactca ttacttagtt cattctttgt atcctttata
    22141 cttgcattta cagattttgg agcagctaag gtagttggag gaaactacaa tgtactggct
    22201 actgacatat ataaacaagt agttggacaa tttaacatac caatgggagc tactgtttct
    22261 atggtaatgt taattcctgt actaatagca tttacattag ataagatggc aagtaagaaa
    22321 caaggtatga ccataactgc aaagtctatg ccatataaga ttaaggaaaa taagtttaga
    22381 gatagagtat ttttaatagt atgtagtttt ataacattgc taatttttat tttaatattc
    22441 gtttcagtta taggttcagt tataaagtta tggccatata atttaagctt tacaatggaa
    22501 cactatacat ttaaaggtat tttgggaagt ggaataaaaa catatttaag cagtttgaaa
    22561 atatcaactt taacagcaat atttggaact atatttgtat tttttagtgc ttatatgata
    22621 gaaaaggtgg ataagtttcc taaattaaga caaacagctt accttttatc aatgatacca
    22681 atggccttac ctggcttagt tttaggtata tcatatataa ctttttttaa ctcagcaagt
    22741 aatccattaa attttttata tggaagtaca gcaatattag ttatagtaaa tgtagttcat
    22801 ttttattcag tttgttttat aacctcaaat tcatctttaa aaatgttgga taaagagtat
    22861 gaattagttg caaaatccat aaatattcca ttctataaag tattttttaa tataacagtt
    22921 ccaatgtcta taacatcaat acttgaaata gtagtatatt attttgtaaa ttcaatggtt
    22981 actgtttcag cattaatatt tttatataca cctcaaactc agactgcatc aatatcaata
    23041 ctaaagttag atgagatagg atatatagga ccatcagctg ctatggcagt attagtgctt
    23101 ttaacaaata taatagtaag acttttgtac gaatttgtaa caaagaaatt aaaaaataac
    23161 acacaaaaat ggcagcaaaa agatgttgca taggggaggt aaaaatgaaa tttaagaagc
    23221 tagtttcttt aggagtatta tcaacacttt tattaagtgt aataacagga tgtagtactg
    23281 attcatcagg ttcagattca aagtctgata gtaagacaaa aggggattta acagtttaca
    23341 cagcaattga agaagatagt atagagccat atttagctac atttaaagag aaatatccag
    23401 atattaaatt aaatattgtt agggcatcaa caggagatat aacagcaaga ttattagcag
    23461 aaaaggataa tcctcaagca gatgttgtat ggggagttgc agccacaagc ttattggttg
    23521 cagatgacca ggggatgtta gaaccatatg ctccaaaagg ttctgaagaa atagagtctg
    23581 catttaaaga tgataaagaa gttccttctt gggttgggat agatgcttgg atgacaggta
    23641 taacagtaaa cactaaagaa ttaagtgata agaacatccc agttcctagt tcttacgaag
    23701 atttaataaa gccagaatat aaaggtctta tatctatgcc taatccatct tcttctggta
    23761 cagggtactt aacagtatca gcccttatac aaattatggg ggaagaaaaa gcatggcagt
    23821 atatggataa gttacatgag aatattggtg tatacactca atctggttca gctccagcta
    23881 catcagcagc ttcaggagaa tatcctattg gtatatcttt tggatataga ggtataaagt
    23941 taaaagagga aggctatcca gtagaagtag tatttccaaa agaaggttct ggatgggata
    24001 tagaagcaaa tgctcttgtg aaaaaagata atataaaaga agcatcaaaa gtatttttag
    24061 attgggctat aagtaaagat gcaatgaacg aatactctaa aaactatgca gttactacta
    24121 tttctacagg aaatccaata ccagaaggat tccctaaaaa gccattagag caaatgatag
    24181 ataatgattt aaaatcagca gcaaaaaata gagaagatat actaaataaa tggatatcaa
    24241 aatatgatgg taagacagaa aaagagagtt aagtaaaact atacaaatgg agtaaaaaga
    24301 aaaatttttt ttaagtagat tttattatta taatataagt tgagaataat ataggtcaag
    24361 aaaagtgaat attctataaa aatgtataga gtaagtatac cattcagttt gaatggtata
    24421 ttttttattt ttagagatta atttagaaat agatatacta cagtttaaaa tagtatattt
    24481 taatttcatt attaaagtat agatgttata tataaagtaa ataagatatt aaataattat
    24541 aaactaaagt aaataaaata ttgagcgatt attaaaaaga tatatatata atttaatttg
    24601 agcagtttgt tatatattat aaaaaaagag aaaaataaaa ggtaaattgt ttctggataa
    24661 cttaaaattg gaagtaagaa tatttaacta gagaagaatt atttaagttt aggttagctc
    24721 taggagtagc tgattttata ggaacaatag attttatgga tgatgatata aacatggtag
    24781 caattagacc agaagatata agggtagaat ttaatagaga tttcaaagaa attatagacc
    24841 tcaaactcag accgtatcga tataaaaact aaagttagat gatatagtgt tgtagatagg
    24901 accatcagct gttatggcag tattagtgct tttaacaaat ataatagtaa aacttttgta
    24961 tgaattgata ataaaaaaat taaaaaccaa gacataaaaa tggcaacaaa aagaagttga
    25021 ataagggagg taaaaatgaa atttaagaag ttagtctctt tgggaatatt atcaacactt
    25081 ttattgagtg taataactgg atgtagtaat gtttcatcag gttcaaactt aaagtctaat
    25141 aataagacaa aaggagagtt aacagtttat acagcaattg aggaagatag tatagataca
    25201 tacttagcta catttaaaga gaaatatcca gatattaaat tgaatgtact tagattatca
    25261 gtaggaggta taacagcaag attattagca gaaaaagata atcctaaagc agatgtgtta
    25321 tggggtgttg cagctacaag tttgataatt ttagatgaca aagaaatgct agagccatac
    25381 gctccgaaag gctttgataa ggtaaattct gcatttaaag atgataagaa agttccttct
    25441 tgggttggaa tagatgcttg gatgactggt ataacagtaa atactaaaga attaaaagct
    25501 aaaaatatat cagctcctag ttcttatgaa gatttaataa agccagaata taaagatctt
    25561 atatctatgc ctagtccagc ttcttcagga acaggatatt taacagtatc agaactcata
    25621 caaattatgg gtgaagaaaa agcatggcag tatatggata aattgcataa aaatattggt
    25681 gtatacactc agtctggttc agctccagct atattagcag cttcaggaga atatcccatt
    25741 ggtatatctt ttggaaatag aggtataaag ttaaaaggtg aaggttatcc agtagaagta
    25801 gtatttccaa aagaaggttc tggatgggag atagaatcaa atgcacttgt gaaaaaagat
    25861 aatataaaag aagcatcaaa agtattttta gattgggcta taagtaaaga tgcaatgaat
    25921 gagtattcta agaattttgc agttactaca attgacacgg gaaatccggt gccagaagga
    25981 tttccaaaga atccattaga acagatgata gataatgatt taaaatcagc agcaagaaat
    26041 agagaggcta tactaaataa atggatagaa aaatatgatg gaaagacaga aaaagagagc
    26101 taagccttgc aataaaattt agtacaaaaa gcgcctagaa tataagtttt atatctaggc
    26161 tcttttgata tatttattta aaatgcatat ttctatataa aaataaatag tttagtacct
    26221 tgattaaatt aaataaaaac cttaaaataa agagatgtaa agaaaaacta aaaacgaata
    26281 aaaggtgaat ttatatgata aaagaaataa tagtagtaga gggtagagat gacgttacag
    26341 cagttaagag agcaatagat gcagaactta taactacagg tggatttgga tttccaaaag
    26401 gtgtaatgga gcgtataaaa gctgctaatg aaagacgtgg tgttataata tttacagacc
    26461 cagattttgc aggtgaaaaa ataagaaaaa aaatagcatc agaagttcca ggatgtaaac
    26521 atgcatttct accgagagag gaagctaaaa aggatggaga tataggtatt gagaatgcta
    26581 ctcctcaaag tataatagca gctctgaata aagttagaac tgaaaataca gaaaaaagag
    26641 atgaatttaa gcaagttgac ttaattagaa atggtcttat aggtaatgaa gaagcatcac
    26701 ttagaagaga tgcacttgga aaaatacttg gaattggata tggaaatgca aaacaatttt
    26761 taaatagatt gaataattat ggagttgaaa gagaagaatt tatagatgca ttaaaacaaa
    26821 tctaaacgta atgtttaatg tatgaaaaat atgtatataa aataaattgt ggaattacgg
    26881 ttaatttaag atgaattatt tataatatac accttatttt aaatcaatgt aaatacatat
    26941 aaataagtat aattaataaa attaaatatc aaaataagtt aaaaaggagt aaataatgga
    27001 tagactatct tcacataatg caacaaagga agtagtacaa aaatataatt ttaaattttc
    27061 aaagtcttta ggtcaaaatt tcttgataga tagtaatata attgataaaa tcttatctgg
    27121 agctagaatc acaagaggag ataatatcat agaagtagga cctggtatag gaactcttac
    27181 tcgtgagatg ggtaaaatag cagaaaaagt agtggctata gaaatcgata gaaatttaat
    27241 tcctatattg aaagatacct tgtcagattt agataataca gaggttgtaa atcaagatat
    27301 actaaaagta gacatacagg aattagtaaa agataaatta aatggtgggc cagttaagct
    27361 tgtagcaaat cttccatatt atataactac tccaatagtt atgaaatttt tagaggaaga
    27421 catacctgta acagacatag tagtcatggt tcaaaaagaa gttgcagata gaatgaatgc
    27481 tattccgggt actaaagatt atggggcatt atctatagct gttcaatact attgtgatac
    27541 agagattgtg gcaaaagctc caagacatat gtttatacca caaccaaatg tagattccac
    27601 agttataggt ctacatgtaa gagataaaag aaaatatgat gttcataatg aagacatatt
    27661 ttttaaaaca gtaaaagcat cctttggaca aagaagaaaa actcttctta attcgttggg
    27721 aggtcttggt tttcttaata aagatgaaat aagagaagta ttaaaagaag ctaatataga
    27781 cgaaaaaaga aggggtgaaa ctcttagtat agaagagttt tcagttcttt caaatataat
    27841 aaatacaaaa gtatcttcta aatagaaggt acttttttta attttgttaa catatattgt
    27901 ttagagaaac tgtgttaagg ggggattgca gtgggttcta aaagaaagtt taaaagagac
    27961 tatataatac tagaagctaa agacataaac tttagataca aagaacgagt actgccaaaa
    28021 gcttttgcaa aagttgaagt aaatgatgaa aaagcaatta ttgcattata tgtagaaaat
    28081 ctaaaatatg ttaaagatgg gtataaagca gtagcaataa aagatgacca tggattaata
    28141 gacttaggaa atataatttt aaatgaacaa ggaagaggtg aatttgtttt agatttagaa
    28201 gattatgatg atgagataaa aggtattgca ttattgtatg aaagaaatat acctttaatt
    28261 ggatttaaag gaaataagct tgaaaattat gaggacatat tatttcaaga ggtagaggaa
    28321 gaagaatatg aagaatatga agagttggaa gaagaagacg aagagataga ggagaatgag
    28381 gaaagtgata gagacgaaga tagtgaagaa gaatatatta tagaagaata tgatgaagat
    28441 gagtatgaag actattggga agatgatgaa gatattgaag aaggagagtt aaaagcaaga
    28501 acggaagtag aagattctga tgatgaggaa atagaagagt tcaataatta taaggaaata
    28561 gaaaattata atagggttaa agtacaggaa gaatatatag acatagaata tgatgaagaa
    28621 gaagaggaag aagaagtttt tgaagtaaat agagaagaag ataggtttgt ttcctcaaaa
    28681 cctactaaat caaagaaagt tcaaaaagat cagaataata ttgaaaaaat aaaaagagct
    28741 aatggagtaa gtcaaggaga gagtggaaat aaacaaacca gtacattact tatgccaaga
    28801 caaataaaaa aaggtttgaa attatttaaa gaagtaaaac cttttgtaac agattatata
    28861 gataagacaa gatggtggaa aatagagata aatcctacaa cactgtgtgg atatacaatg
    28921 ccttatttag gatatttaaa cgtattgaat tacactatgt atagtgatgc agttttacat
    28981 tcatataaat acagacacta tttatttgga gttcagtatg atgaatataa taagagaaaa
    29041 cattatattt atgctatccc aggaaataga aatgaacagc cagacaatgg aagcacagga
    29101 tttttaagat atcaagcttg tgataataga acaaatagtt taggatattg gctatgtttt
    29161 atagattcta gacaaagaaa tatagttagg taaaaataat aaaaatgaaa aaatttagac
    29221 tcaattctta ttttactgaa aaaattaatg aaatgtagta caaaacttta aaaactattg
    29281 ataatattta aatattacac tacaatatat cttgaaatac ctattatatt atggaggaaa
    29341 attatgataa acgagcaaag aattttaaat gagtttcttg aattagtaca gatagatagt
    29401 ttatctttaa aagaggggaa tgttgctaaa gtattagtta aaaaattaga agaaataggt
    29461 tgttcagtag taatagacaa tgctggtgaa aaagcaaatg gagaaactgg taatataata
    29521 gctacattaa aaggaaataa agaaggaaag aaaatattat ttagttcaca tatggataca
    29581 gtaactccag gtataggggt aaaacctata gtggatgaag caaatggaat aataaaaagt
    29641 gatggtacta caatattagg ttctgatgat aaagcaggta tagcagctat attagaaggc
    29701 ttaagatata taaatgaaaa taacatagaa catactgata tacaagttgt attctctata
    29761 tgtgaggaat gtggattagt tggagctaaa aatttagact atggaaaaat agattctgaa
    29821 tatgcattta tattagatag tggtggttct ccaggtgaaa taatagtaaa agctcctgca
    29881 caagatgtaa taaatgttaa gatattagga aagactgctc atgcaggtct tgaacctgaa
    29941 gctggaataa gtgctataat ggtagctgcc agagcaatag aaaatatgaa tttacttaga
    30001 atagacgaag aaacaactgc taacatagga attataaacg gtggaacagc tacaaatata
    30061 gtaacaggag aagttaatat agttgcagaa gccagaagtc taaaagaaaa taaattagat
    30121 gttcaaacta aacacatggt agagactttt gaaaaagctg cgaaagattt tggagctcaa
    30181 attgagatag atgttaatag agcatacaca ccaatagatg ttagtgagga ttctgaaata
    30241 ataaaacttg caaagaaggc tttttcaaac ttaggtatag aaggtcatac tgaatctaca
    30301 ggaggaggta gtgatacaaa tatattaagt aagaatggta tcgaagctat aactttagga
    30361 attggtatga aaaatgctca tacactaagt gaacatatag ctataaaaga cttatatgat
    30421 tctgctaaaa tggtagtgga gataataaag gaagcataaa tttaaataat agtgtataat
    30481 atatatatcg cctagcttaa gctaggtgat ttttcatttt atagaagatt gataatctct
    30541 attgaaagca aagagtatac atttgcatat aagagagtgt ttaatttatg ttcaaatgta
    30601 ggttcttaat aatatttaga ttgtttcata agggaggaat aaatatggaa acaaggtatg
    30661 aagaagcaaa taaaataaca atacaatcaa tactttggaa tatagtatta acaataatta
    30721 aggttatagc tggtgttatt ggtaattcta gcgctatgat tgcggatgga ttacattcag
    30781 catcagatat tataagttct ataggagtac taataggcaa ttatgtatct tctagacctg
    30841 gagatagaga gcataattat ggtcatgaga aagcagagac attagtatca tttgtgttat
    30901 caatattgct tattttcgtt tctataacaa taggtataga ggcaattaaa tctttattta
    30961 acttagatgc attaagtgtt ccatcaatat taccattagt agtatcagtt atttcaatcc
    31021 tgataaaaga gtatcaatat agaataacaa taaaagttgc taaaaagata aattcaccag
    31081 ccctaaaagc tgatgcttgg catcacaggt cagatgcttt atcttcagta gctgctttta
    31141 taggtatagg tggttctatt ttagggttta aaccattaga cccaattgcg tcagttgtag
    31201 tggctatatt tgtagctaaa gtaggaatta gtatacttat aagttctgtt aatgaattga
    31261 tggatgtttc agttgatgaa gaagagataa aagaattaaa atttattgta gcagatacag
    31321 aaggtgttaa aaatcttggt gatataaaga ctagaaaaca tggagctatg gcatatgtag
    31381 atttgactat atgtgtggat gaaaatttga cagttaagca aggtcatgat attgctacca
    31441 agcttgaaaa acatataata aaacatatgg aatttgtaaa aggtataaca gttcacgttg
    31501 aaccatgtac taattgccaa ggaaataagt gtaataatta attaaaactt aaaaaaaagt
    31561 gtaaaaagtt atataattgt tttatgatta taaactctag gggtggttaa atgagcaaga
    31621 aaagaaaact aaagaaaaaa gctgcaatat taataggagt agttgttttc tttataatat
    31681 actttatatc ctttagaata ggaataaata tggaaaagga taaaagtgaa gtgccaaaga
    31741 aggtagaaaa agaagaagaa gataataggt ctttaaggac tcaaataaaa gagaagaaga
    31801 aggtttatat atcagataaa aatatagaaa acataaaaat tgaagaagat atgtgggatg
    31861 aaataaagtt atttttctca gattttaaga aagtaagaaa tactacttct tcttacgaag
    31921 cagtttatga aggttattct gatgatggag ttaaattttc tactgacctt aactttttta
    31981 gagtatatac tgtaaataaa gaagaatatt ataaagtacc agttagttct aaggatgctt
    32041 ttaagaaact tttagatgag agtatatata cttcgtttga ccttttaagt cattataaag
    32101 attggaaaga ggttactata actcatggaa aagaaattaa aaaagttcat agatggaaat
    32161 ttgatgattt agcacataag atgatttcaa agagggttgt aggaaaagtt caacctgaga
    32221 aaagtaagga aagaagtgac tataatttta ctataaatat aaaaggtgat aattttgatg
    32281 taaaagttga tacaatggga aaagattatg taaaagttac ttctaagaaa aatgtatctt
    32341 actatgaggt tcatacaagt ttatttgatt accttaaaaa tgagatattt gaaattggaa
    32401 agaaataaga ctttaagaag gctatcaaat tagatatttg atagcctaaa ttattttata
    32461 tattattttt caaccaacaa gtctgcaacc ttataaattg gaccagcacc agcagttatt
    32521 actaaatcat tatccttaac attttcacgt aagtatttta ctatatcttc aaattctttt
    32581 atatagatgg catctacatt gttttgatat aatttctcaa ccaagtcctt agaatgtata
    32641 tctcctgggt ctttttccct agcagcatat atatctgtta tgataacttt atcagcagca
    32701 tagaatgcct ctgaaaattc acctaaaaga gattttgttc tagtataagt atgtggctgg
    32761 aagatacacc ataaagttga tttttttaat tttttggcag cagctaaagt tgcttttaac
    32821 tcagttggat gatgtgcata atcatctact actaaagcat tcttatagta ccctttagtt
    32881 tcaaatcttc ttccgacacc tttataaatt tttatatttt ttcttatagt ctctaagtca
    32941 ataccagaaa caagagctgc cattatggca gaagaagcat tgtatatgtt atgaagacca
    33001 tatacagaaa gttcaaattc tcctaaatct cttccttcat attctattct aaatattcca
    33061 tgaccactat tgtcaaagtg aatatctttt attacagcat cattgtcaga atctctgccg
    33121 tattttataa tagtagcttt aacatcatac aatatgtcat caatattttc atcatcgcca
    33181 tttattatga aatagccttc tgggggtaat aattttccaa atttgttaaa agaagccttg
    33241 atttcatcta ttccagagaa ataatcaaga tggtcttctt caacatttaa aactatagat
    33301 atctttgggt taaaattaag gaaactatct acatattcac atgcttcagt tataaagtgg
    33361 ttggaattac ctatcttaac attaccacct atcatactta agttaccacc aactagtata
    33421 gttgggtcta agtctgcata ttcaaagatt gtagataaca tagatgttgt agaagttttt
    33481 ccatgtgttc cagaaactgc tatagaattt ttatattccc tcatgatttg gcctaaaaaa
    33541 gttgctctgt tcattaccaa tttatttttt tctttagcag ccacaagttc ttcattgtct
    33601 ggatgaactg ctgctgtata aacaaccata tttacatcat ctgatatatt atcttttttc
    33661 tgaccaatat aaatagttgc cccttggtcc ctaagtttgt ctaataggta tgattcgttt
    33721 gaatccgaac ctgaaacttg ataaccttta ttgatacata tttcagctaa tgcactcatg
    33781 ctgataccgc caattcctat aaagtgtata ttcataataa aaccaccttt ctttttaatt
    33841 aagtcctaca atatgaacta tttctgtggt ataataatat aatgttcaat attttataat
    33901 taatatgcat ataatattat atgtatttta tttttgaata taataaaaca taagtaatat
    33961 tataccacaa taagaattgt tatgcattag ttagtttaac gttatataaa ggtacttgta
    34021 ggaggaaata ataatgaaat ttaaaagaac agagagaatt ggagcaatag ttaaaatact
    34081 gtctgataat ccaaataaaa tatatacttt aagttatttt acaaatcaat ttaatgcagc
    34141 aaaatcaaca ataagtgaag atttattagt tgtaaaaaat gtatttgaga agctacattt
    34201 aggtaaagtt ataactattt caggagcagc aggaggagta aaatatatac ctaaaacttc
    34261 tatagcagaa aatgcagagt tcttaatgga attatgcgag agaatatgtg acaagtctag
    34321 aatactatct ggtggttttt tatacttgat tgacctaatt tacgacccta caatagctgc
    34381 taagatagga aagatattcg catcaaatat tgagtatgta gatgcagatt atgttgtgac
    34441 aatggaaaca aaaggtatac ctatggcact tatgactgca aaagctatga atttacctct
    34501 agttataata agaaaagata taaaggtatc agaaggtcct acacttagca tgacatatgt
    34561 gagtggaaac agttctaaag ttgaaagtat gagtttgcca agaaaagctc ttaaaccaga
    34621 tagtaaagtt ataattatag atgatttcat gaggggtgga ggtactataa aaggaatggt
    34681 agaccttatg aatgaatttg gtgcagaggt tataggtact ggagtattca tatctacaac
    34741 gaatccttca gaaaaaatgg taaaagatta tatttcattg attcaattgg atgttaatgg
    34801 agataaaata gtagtagaac caaacttaaa gacttttaaa gatgaatata gaaatgaaga
    34861 tttagacgaa gaagaagatt cagaatttga atttgagata gatgaagatt aataaaaaaa
    34921 atatttagaa aaatatgtaa aaataaaaag aggatatccc tagttgttca tagaattatt
    34981 taaaaaacag tttatattaa tttattccag cgataataat tttaatataa attaatttct
    35041 gtcaaaatca aagggggata ttttggtatg aagataactg acgtaagagt aagaaaatta
    35101 acagaagaag gtaagatgaa atgtatagtt tcaataactt tcgataattt gtttgtagtg
    35161 catgatataa aagttataga aggccataat gggctattta tagcaatgcc tagcagaaaa
    35221 gtaggcgaag gaaatttcag agatatagct catccaataa atgcagaaat gagacaagtt
    35281 ttagaagatg cagttttaca agcgtatcat gaagcattag ttcaatggga agtagctgct
    35341 gaataataat aaatatataa aatggagaga gcccgaacat ttaggctctt tttaaatgta
    35401 gaatttctat taaaatatgt actttttagt aaaaaagtgc tatattatta gtgataatgt
    35461 atatgttgta tacaaattgt tagataaatg tattataatg tatattgatt aagagaataa
    35521 tacgaataaa attaaattag attgaatgga gtgtatcaat gaactttaaa gctataatcc
    35581 ttgctgctgg caaaggaaca agaatgaagt ctaaataccc aaaagttata cataaagtat
    35641 gtggaaaaga aatggttaac catattatag atgtttcaaa aaaatcaggg gtaaaagata
    35701 ctgtcgtgat tttaggacat gaagctgatg tagttaaaga aaagcttgca gaagaaatta
    35761 taatagccat gcaaacagaa caacttggga caggacatgc tgtaaagatg gctaaagaat
    35821 atattaatga tgaagataca atagttgtac tttgtggaga tactccactt ataaaagaag
    35881 agacactaaa gagactattt gaatatcata tagaaaataa atatcatgct acagttctta
    35941 caacaagagt tggaaatcca acaggttatg gtagaataat aagagataag aagggtgacc
    36001 ttcttaaaat agtggaacaa aaagatgcaa attcagaaga aaagatgata agtgagataa
    36061 actctggaat ttattgtttt aatggaaaga gtcttagaga agcactagat ttgctaaata
    36121 ataacaattc acaaggtgaa tattatttaa ctgatactgc taaaataatg agagataaag
    36181 gattaaaagt tggtgcattt gcaggttcaa ctattgaaga acttatgggc gttaattcaa
    36241 gagtagaatt atcaaaggca gaagaaatta tgagacgaag aataaatgaa tctcatatgg
    36301 taaatggagt aactataata gacactaact ctacctatat agaatctgat gttatgatag
    36361 gcaatgatac tataatatat ccaggtgtta tgttacaagg aaaaacaagg ataggttcag
    36421 attgtataat tggaatgaat tcatctataa caaattcaga aataggtgat ggtacagaaa
    36481 taaaaaattc aactataatt gatagtaagg ttggagaaaa ttctactgtt gggccatatg
    36541 catacttaag accaaagagt gacttaggaa ataatgtaaa aataggcgat tttgtagaag
    36601 taaaaaatgc tattatagaa gatggctcaa aagcatctca cctttcttat ataggagatg
    36661 cacatgtcgg taaaaatgta aatattggat gtggagtagt atttgtaaac tatgatggta
    36721 aaaataaatt taaatcaata gtaaaagaca atgcgtttat aggttcaaat tctaatttag
    36781 ttgcaccagt agttgtagaa gaaaaaggat atatagcaac aggttcaaca ataacacatg
    36841 atgtcccaga tggtgctttg gcaatagcaa gagaaagaca agttatcaaa gaaggatggg
    36901 tagaaaaaaa gaatcaaaaa gacgaccaaa gtaagtaatt atgtagttta ttattttggc
    36961 taaaaaattt agctcgaaat tattataaat tatattcatt agtattttaa aattttcagg
    37021 agggttacat aaatgaatac tagcggaagc gagattaaaa ttattgcagg taattcatcg
    37081 aaagagttag cacaaaaaat agctgactat ataggtgtat cagtactaga ttgtgaagta
    37141 ggtacattta gtgatggtga aatatgtgta aacatgaatg agacagtaag aggttgtgat
    37201 gtgtttgtag tgcaatctac taacagccca gtaaatgata acttaatgga gttattaatc
    37261 ttaattgatg cgttaaagag agcatcagca ggtagaataa cagcagttat tccttactat
    37321 ggatatgcaa gacaagacag aaaggctaag gcaagagatc caatcactgc taaattagtt
    37381 gcaaatttaa taactgctgc tggagcagat agagtattaa caatggattt acatgcagct
    37441 caaatacaag gatattttga tataccatta gaccacttac taggtggaac aatattagca
    37501 aattatttta atgaaaagaa aatagaagat ttagtagtag tatctcctga cttaggaagt
    37561 gttactagat caagaaaatt tgctaatact ttaaatggag aagttccaat agctataata
    37621 gataaaagaa gaccgaaagc taatgtatgt gaagttatga acttaatagg tgatgttaaa
    37681 ggtaaaaatg ttatactatt agacgatatg atagatacag caggaacaat agttaatgct
    37741 gcaaatgcac ttaaagagtt tggagcaaaa gatgtttatg cttgttgtac tcatggtgtg
    37801 ttatcaggtc cagctattga aagaattgct aattcagaaa taagtgagtt aatagttctt
    37861 gatactatac aacttcctga agagaaaaga atagataaga taaagataaa aacagtagct
    37921 cctttatttg gagatgctat aagaatgata ttctctaatg aatcagtaag taaattattc
    37981 taatttattg tatttatgag agttgtttaa aaggatataa accttttgag caactctttt
    38041 ttgattttat acttatggaa tgttattttt ttcttgttag aaaggtatat attattgtaa
    38101 aaagagatta tagtttaaaa ttatgtatat ttaagtaaaa taaacctaat aaagggtata
    38161 ttattcaaaa tattatgaat ataaaaaata ttacaaaaaa gtattgaaaa aattcagatt
    38221 atgtagtatt atgtaaatat aaataaagaa agaggtgttt ccaatgggaa ggttagaaga
    38281 tgaaccccta ataattaaat gattcagtca caatctaaag tgaatataaa aatctattga
    38341 aagtttattt ataagaattg aaaatagtat ctattctatc tttagtgttt gtgagagggt
    38401 tctggattat ataagattac tgtaaagagt aattttgcac aataagcata tctgtaaatg
    38461 aagagtttcg tttatataat gattgtggct gaatcataac tataatagat agaatgttta
    38521 taatagatga ttttataaaa tactatattg taatagcttt tagctttcaa ataaatatta
    38581 gcaaaggatg gaaatgacaa tgataaaact aaatgataat ttataaatat ctccttacga
    38641 tatataataa ttgtagggag atgtttcttt ttttgtatag cataattttt ctagaaattc
    38701 tgtttcaata tgtaaaaagt tggtatatat aaatagtata aacagtgttg ctaaaatatg
    38761 gtagatatta tataatataa gtggatatgt atatacatta tgagttatat aatttgaatt
    38821 taataaaata gaaacgttga tttatgttct gtaatgtggg caccttggag catattgagt
    38881 tagtggtgca accggctatg aaattgtatt tatttataaa tactattatt ttcatagctt
    38941 tttttattta aaaaatggtt atatgttaga aataaatttg gttagtacgt ttaaataatt
    39001 ttacttagat ttttaaccag atatattgta agtaagtatt aattaaaaaa tttaaggggg
    39061 aataaaaatg aagttaaaaa agaataaaaa aggtttcact ttagtggaat tattggtagt
    39121 aattgcaatt ataggtatat tagcagtagt ggcagttcca gctttattta gtaatataaa
    39181 caaggctaag gtagcaagtg ttgagtctga ttatagttca gttaagagtg cagcattatc
    39241 ttattattca gatactaata agataccagt tacaccagat ggtcaaactg gtttaagtgt
    39301 tttagagact tatatggagt ctctgcctga taaagctgat ataggtggaa aatataaatt
    39361 gattaaagtt ggtaataaat tggtattaca gataggtaca aatactgaag gagttacatt
    39421 aacagaggca caatcagcaa agttattgag tgatataggt gaaaataaaa tatatacaag
    39481 cgctacaaca aatagtttgg gaaatccatt aacaagtaat acaaaaatag ataataacgt
    39541 tctatatata gtacttatag ataatactgt gatggacaca acaaaatagt taaaacaata
    39601 tttttataaa aatattgtaa atttaatgaa aaatataata taaaagacag gaagaaaatc
    39661 ttcctgtttt tttatactat tttctttaat gttcaattat gaatgatgat ataattgaga
    39721 tagtaaagga gtgaaacctg tggctaaaaa agttagaata ggagacaaac tggtcgaaaa
    39781 aggatatata acagaagaac agcttaaatg ggcactatca gaacaaaaaa acagtggaaa
    39841 aagactagga gaatttctag tacaagaagg gcttatagat agtaacttac taataagtgt
    39901 attaaaagaa ctcttggata tagaaagtat atttttagaa gggacagaaa tagacacttt
    39961 agctacaaaa atggttccag aaaatatatg caaaagatat actgtatttc catttaaaat
    40021 tgatggaaat aaaatatgtc tagccatgtc agacccacaa gatagagaag cagttcaaga
    40081 tgtaaggcgt atgtctggta aagacgtaga gatatttata tcaagtacag aagatataaa
    40141 caaagcaata ggtcatgctt atgcacattc agaaataaat aaggcaatga ctgagtacaa
    40201 caaaaataga actggtggag taagagaaac agtaatactt gaagaagatg taaatgctgc
    40261 tccaatagta cgattagtaa ataacatact agaaaatgct gttagaatgg aagcaagtga
    40321 tatacatata gaacaaagtg aaaattacat gcgtgttagg tttaggatag atggaatgtt
    40381 aagagaatat atgaggatga actcagcacc atataaggca gtaatcagcc gtatcaagat
    40441 aatgtcagat ataaatatat ctgaaaagag aataccacag gatggtagaa tctatttaaa
    40501 agtagataat aaacccatag attttagggt ttctacaatg ccaactaaca gggatgaaaa
    40561 gatagctatg agagttcttg ataagagtaa ttttatggtt agtaaagaag tactaggaat
    40621 agacgaacat ggctctaaga tatatgatga attgataaac actccttatg gtcttatatt
    40681 ggttgttggc cctactggaa gtggtaaaac cactacattg tattcaatgt taaatcaact
    40741 aaatacagaa aatagaaatc tattaacaat agaagaccct atagagtatg aattacctgg
    40801 tgtaaatcaa tctcaaataa atgaaaaggc agggcttact ttcgccagcg gacttagagc
    40861 ttttatgcgt caagacccag atataataat ggttggagag ataagagata ctgagacagc
    40921 agaaatagca attagagctt ctcttacagg acatttagtt ttaagtacat tacatgcaaa
    40981 tacagcagtt ggagcaattt cacgtctttt agatatggat gtggaaagtt ttttaataac
    41041 ttcatctgtt ttaggtgtaa tatctcaaag acttacacga aaaatatgtg agcattgtaa
    41101 ggtttcatat gaagcagata ttggagaaaa aaaggctctt ggtattgatg taaatgaatc
    41161 agtaactatt tatagaggaa aaggttgtga aaggtgtaat aatactggat ataaaggacg
    41221 tctaggtata tttgaaatgc ttgaaattac tccagaaata aaagagttaa tagattcatc
    41281 tgcaaatcaa agagaaattt taaagatggc tagaaaacaa ggtatggtta gcttaaaaga
    41341 ggatatagtt aaaaaagttt taaatggaaa gacaacagta gaagaaatga ttagaataat
    41401 tttgatgact gattaaagga tgatattatg aaaagtttta aatacacagt aataaaagag
    41461 gacggaaaaa agcaaactga tgtgattgaa gccaacgatt ttaatgaagc tagaaatctt
    41521 cttagaaaga gaaatcttag agttattgaa atcaaagaaa gcaaaaataa aaatataaga
    41581 ttatctagta gaaagagaaa aaaagactta ggagctgacc agataagcca cttttgtaga
    41641 cagtttgcta ttatagtatc ttctggtata aatagtattt ctggattaga aacattagct
    41701 agaaggtcaa ctaacattac attaagagaa gaaattaatc gtatagtggc agaaataaaa
    41761 gtaggttcta ctattgcaga ttcaatgctt tcatcaaaat caaagtttcc aaagttactt
    41821 ggtgctatgg tagctacagg tgaagctaca ggtaaattag atgaagtatt aaaaagtatg
    41881 gcttcatttt atgcaagtga acacagggtt aaacaaaagc ttagaaatgc tgcaacatat
    41941 ccattaatag ttttgatttc ttcatttata atgattttta tatttacaac atttatggtg
    42001 cctaagatga ttaattcaat tacaacagta ggagcaactc ttccacttat aacaagaatt
    42061 gttatggggt ttggtatgtt tatgaagaag ttttggataa tagtactttt agttattata
    42121 ttatttatct atcagtttaa aaaatatcta aaaacgtcag ttggaagggc tcataaagat
    42181 agagttataa ataagatacc tgttttagga aaaggaataa attgtatggt agctacacga
    42241 ttttcaagag cattgtatct atttgtatca acaggatacc cattagttca agggcttgat
    42301 tatattatag atagtgtaaa taacaccatg gcagagaaga ttttagcatc agctaaagat
    42361 ggaataacaa gaggagaagg cttagcagaa aatttagaga gatatacgta ttttgactct
    42421 gtgttggtac agatgatagc tataggggag cagacagggg aattagagaa tataagtaga
    42481 cagatggcag aattttatga gtatgaatcc gagatttatt taaataggat ggcctcaatg
    42541 atagagcctg ttttaataat tgcagttggt attatagttg cgatactggt tgtatcaata
    42601 ttcatgccaa tgcttagtat atatgatgcc atgtaggagg gaaaggacaa atgaacaagg
    42661 tttatgtatc atattatggc gattctattt ccatagttga gggcagatat aaacacgata
    42721 aggataagtt ttatgtaaaa aattttaatg tgttttcaat agaggatatt gcacaagatt
    42781 tttctaagga aaagtatgct ctattgagat acgcattaga aaatgttaag ttaaaatcaa
    42841 gacatgtagt tttttgttta aatacaagag atgtaatttt aaaaccccaa aaacttccta
    42901 atatggacaa aaaggattta gacggattta tgaagctaga gatggacgaa atgatggctt
    42961 tggagtcaga tgaatatgta ttttcgtatg aagtatctag tgaaacacct gataattcag
    43021 atggcgatga atcaagttta aatcttatta ttggagctat acaaaaggaa gaggtaaatg
    43081 caattgtaga gataatgcat gaatttgatt tagtattaga tagaatagat acattatcaa
    43141 cagcttactt aagaatgctt aaaaatttag actacaatga tataatggta accaatataa
    43201 gtgacaatag cacaattata aatatatata agaaagatac tttatttatt agtgataata
    43261 tacctataaa gataactgct aaagatatgg acatgcaatt attaaatgta gtagaagaat
    43321 caaaaggtct tatgaattac tattcgtcaa gaaactttgg taaggttacg gatactatat
    43381 taatattagg aaaaagatat tccaataaat taatttatga aacttttagc tctgcatttt
    43441 caagctatgt ttctcaaggc cttactgaga taatggatat atctgatatg ataactggag
    43501 atatagatga agatgatata aattcaattg tagagctttt aggaagtatg atagaaccta
    43561 aagggaaaag agattttgaa aatataaact ttttaccaat ggatatactt agaaaacaga
    43621 taaaagctaa acaaatgaaa aacatactga aaattgctcc aatagttatt ttgattcttg
    43681 ctatacctta tttatgttta attttttcaa ataatttagc aaataaggaa cttgaaaatg
    43741 ttaatggtga aataagaaat attgaatctg catattatca gattggaaat atagaaaaaa
    43801 tgatacagag taaaagcgaa gaaataaagg tatatgatat gttaattggg aaaaaggtaa
    43861 agtgggaaaa tttattggat gatatagata aaaatactcc aagtaaagtt gaactaacaa
    43921 gtttatcaac aacttatcaa aatcttggaa ctaataataa agctgataat tcaaaaagta
    43981 ataagactac aacagataaa tcaagtaaca atgaaacaaa atcaagtacg aaaagtgata
    44041 gtaatggaga taaaacagat aatgatttaa atgaagataa gactacaaat gtgtcagaag
    44101 cattaactgg aaatagtgag actaatagta ataacacaga taaagatgat aaaactccat
    44161 tgtatgataa gataccaaat gttataaata tagaaggaaa tgctaaagat tcctcttatg
    44221 taggtcaatt tttatataaa ttaaagggat tatactattt caaggatgtt aaattacata
    44281 gcataaaaag taatgcagaa gattctaact atacatttag tatgacatta tacctgaaag
    44341 aaggtgtatt aactaatgaa taaagatata tttaaaaaag atataaaaga tatatttaaa
    44401 atggaagtta gtctaaaagg gattcttata aaggttataa ttttattagc tattttatca
    44461 tatggttatt ttggacttta ccctcaatat cagaaatata ttgctacaaa aaattctgta
    44521 aaacaggcta ctgaaaaatt aaatatatat aaagagaaaa ctgcacttat gccaaaatat
    44581 gaaaaacaac ttgaagaagt tgaaagtgaa ttaaaagaaa aaagcaaaaa tcttttatat
    44641 gatatgcaag atggtctttt tttaataggt ttagataaaa aaatgaaggc ttataatatt
    44701 gacttaacca gttatgacgt tggaaatgtt aaagaatacg aacattttta tggtatatca
    44761 acaacaataa gtgttagagg tgattataga aatgtcaaaa aattaatggc ttatttagaa
    44821 gaacaaaaaa atgttactca aatactagat tatacaatga cttcatatat agaaccagaa
    44881 aaagaagagg aagaaacaac aaacaataag gatttaatta aaaatgtgaa cataaataaa
    44941 gatacaaaaa tatactgggt ggaaggcgaa aataagtggc atttatataa ggattgtcca
    45001 ttgataaaag agggagagat tctaaaaaat gacaaacttt tagataggtc tactaattat
    45061 ctatgtaata cttgtgtact tagagctgca acagaaaaag aagcaaaaaa agaagtcgta
    45121 gctccaaagg cagaaggtgt agtagatgct acatttaaat ttataatgta cactaaagag
    45181 aatactacaa tgatacttga tacagataat ccatcttcat ggagaccagg aaaatataat
    45241 ccatttaagt ctagtgcgaa ataaagctaa gtaagcttta aagataggtg atgagtttgt
    45301 acaaaaataa tgaaaaaggt cttactttat tagaagtaat aatagctgtt tttatactga
    45361 caatagtttt aagtatttct tataaagtgt tcaatggtat aacatcagca gtaaaaaagc
    45421 aacagattat tacagatgct caagtaaata ttaatctaat taataagtat ctaaatagag
    45481 atttggaaaa ctgtaaagaa ctaactaaaa ctggttcagg taataattat gaatacaata
    45541 tagagatgcc agataatgtt gtaaaatatg aagtttctat agaaactaaa aaaaatacag
    45601 aggtatattc tgtaacgaga atccaaaaca atacaattga tacagaaaat gaagttagag
    45661 aagagataat ctataatcaa ccactagttc aaaacaataa agaaatgaag gaaacaccat
    45721 ttaaaataga aaagcaaact ggtaaatcta tttatactgt aagtatatac tataatgaat
    45781 cagtgcaaga aagtcataaa aatagcaatt taaataacaa aacttataca tttgatgtta
    45841 tgtcaagaat aggataataa gaatttatta aaaaataagt aggttgataa aatatgagta
    45901 aaaagaatag tgaaagagga ttttccttaa ttgaagtgtt agtagctatg gctattatgg
    45961 gaattgtact atttgcattt tttaatatta tcaatactaa taacaaagca aacactaaaa
    46021 atgacacaga tataacttcc ttaaactatg tccaaagtga aatagaaaat ctaagagaaa
    46081 agataaaaag tggagaattt gattttgata gtttagataa actagaagat ggaactgtcg
    46141 tatatgaaaa attaatagat aaatcaaaaa aagttgtata tgataaagtt cttagtgaag
    46201 gtgatgtgag cttatatgat actccatatg aaaagattac aacaataaaa gatgaagatg
    46261 gtaatttaat cgacaaagga aatattacaa ataagataaa gactattgta gaagataaaa
    46321 gtggtcagat atataaaata gctgtaactg ggaaaagcat gaatgactat tcaagtaaaa
    46381 aagaggttaa aattgtaact gaaatattta aagataaata attttacttg ggggtgagat
    46441 tttgaggaag tggaataaat ttaaaagtga aagaggggca gctttagttt tagttttggt
    46501 tgtagtagca ttgctttcta ttgttggatt gatattttca aatcagatag ctaatagaat
    46561 aaaatctaca aagactacta atgaaggtat acaagcaaag tatttagcag agacatgtgt
    46621 ggaaaatagt attgataaag catatgaaaa attgtatgat gagcttgaga aaatggataa
    46681 tgagtttaaa agtgaaaatc aagaaaaatc catatctagg tcaaaattaa gagatatatc
    46741 agatgaagat tttaataatc aagatgagga aaatattgaa gcagaaagac ttggatatat
    46801 gaataatata aatttctatt taaataaagc ttcttcagac ttggaaaaag ctagtatgga
    46861 attgaaaaag ctatatgatt tagatatgtt ggactataga gacattgaat atgtagatgc
    46921 aaatattatt tcacatagag attctatctt agaaatttgc aagaattata cttctggaga
    46981 tattagtaaa ataaatgaat atattctgaa agaagatata gactcaacta cattgattga
    47041 ggcaaagtta gtgaataatg atattctttt aaaaatgttt ttagaagaaa ataaaattga
    47101 aaatgaacat ttaaattcag ccttttcaca tacttataag gcattagata acatttcttt
    47161 ggcgatgcaa aatatgattg agtatagaca tacgtttcat atagatgagc caaaagtaga
    47221 agtatctaat gggattccag attctcaaca atattatgaa ctaatacaaa atcctataat
    47281 aaatagtatg gagtatatct ggaatagtaa atgggataca ttagaaaaac tattggaaat
    47341 tttaccaaat caaactcaag gatttaattc tttaagagtt catttgagaa ataatgtaag
    47401 aaaatttgag aagttaagcg ataatatatc tagtggtaaa aaaaatacag caaaaaattt
    47461 cttaaagtat aaagagttat tgtatgaaat atcagaccaa tgtaatcaac taaaaagtat
    47521 gtcttatgag aaaatacctg ttaaatatga taacatggct ttgataacaa cttttgacta
    47581 tatacaaaat gagcttttag cagagataaa atgcagatta aaagaattaa aaccacaaga
    47641 aattgataaa acagaaggta ttactataaa aatacctttt tataaggctg actacgacat
    47701 gacaaaagaa ggatggccta aattaaaaga aaatggttca ggggcggagt taagcttaat
    47761 ggtaactggt gataaagatg gaataaaaga agtagaagtt acagatggta aaaaaaatat
    47821 tattggttta ggtgttgaag aaaattcaaa ttcaaagtat aaggttgacg ccattgtaaa
    47881 ctttaatcta aatatagata caaatgtagt tggaaattat gatataaaag ataaaatttt
    47941 gattaatcat gatataagtt catacaaaaa agtaaactaa atataagaag caacttagta
    48001 taaataaata ataaaataat tttattattt actactaaaa aaataagtaa actaatagaa
    48061 gatatggaga gtaaatatta tgtatattta tgatttatta gagcaaggta taagactaaa
    48121 cgcatcagat atacacataa cagttggaac aaatcctgta gctagagtaa aaggtggatt
    48181 tgtaaaacta agtgaacaaa tattgacatc agaagtaaca atgcaaatgg caaaagacat
    48241 agcaggggaa tctatgttta aagtaataga agaacatgga gaagctgatt tttcagcttc
    48301 tttaaaaaca ggagaaagat ttagggtaaa tgcatatagg caaaaaggaa attacgcaat
    48361 agcaataagg actataacag cagaaattcc aacatttgaa aaattaggcc ttccagagag
    48421 tataaaatct tttactgaaa agcataaagg attagtttta gttacaggtc caacaggaag
    48481 tggtaaaagt acaacacttg caagtatgat aaatataata aatgaaaaac aacaaaaaca
    48541 tataattaca cttgaagacc cgatagaata tgtacatcat cataaacaaa gtttagtaaa
    48601 tcaaagagaa gtaggaactg atacagaaag ttttcattca gcccttagag ctattttgcg
    48661 tcaggaccca gatgtaatac taataggaga gatgagagac cctgaaactg tttcaatagc
    48721 attaacagca gcagaaacag gtcatttagt attttcaaca ctacatacag tgggggcagc
    48781 taagacaatt gatagaatag tagacatgtt tcaaccatcc caacagcaac aaataaaaac
    48841 acagttatct acagtttgtg aaggtgtggt atcacagcag ttacttccta cagcagatgg
    48901 gaaaggcaga atagcagcaa ttgaattgat gtttgctact ccagcaataa aaaacctgat
    48961 aagggaaggc aaaacatatc aaattccaaa catgatacaa actggagtta aagtaggtat
    49021 gaaaactatg gaccaagatt taatggaact ttataaaaat ggaaagatta caaaagatat
    49081 ggcacttagt aggtgtactg accaagagtt tatgacaaga atgataggtg gagtaaacta
    49141 caatggatat tataatcgtt aatctatatg tatttatggt gggtatagtg tttggaagtt
    49201 tctttaacgt ctgtattttt aggattccag aaggtatatc tatttctaga cctccttctc
    49261 attgtatgag ttgtaatact agactaaagc caatagactt aataccagtg ttaagctatt
    49321 taatgagtgg cagaaagtgt agatattgtt atgagaaaat ttctagtaga tatgctatag
    49381 tggaattaat tacaggactt ttatttttaa cagtatttat gttatatgga atatctacat
    49441 caactattta ttatttggtt cttgtgtctt tactgattat aattacattt atagatattg
    49501 accactttat aataccagat aaaatattgg tttttggagc tatattttct atgattttta
    49561 accttttatt taaagaaatt ccagttaagg attcaattct aggtgttttt atttgtggag
    49621 gaagtgtctg gattatagtt cttttgattg aatttgtaat aaaaaaagag tgtatgggtg
    49681 gaggagatat caaactattt gcaatgctag gtttttacat gggtgttaaa aatggtcttt
    49741 tgacagcctt actaagcgtt tatgtgggag cagtctatgg aatctgtgta ataattataa
    49801 gtagaataaa aggaaaggaa tataattctg ttataccata tggacctttt atatctattg
    49861 gagcattaat aacaatctta tgtggaaagc aattacttga actttatttt tctatggtta
    49921 tatagatgat agtcaagtta aataaagttt taatttaaaa aaatgaatta aagtatttaa
    49981 taaggacaat attattatta accctattac taaagtgcta caaactatgg agtatataat
    50041 aataatttcc ttattttttg cacatttgat aaataaatct ctatgtgttt tttgtgatag
    50101 agaaaagaaa aatttataca atacgttttt tatttacata ataactgtag ttttatctgt
    50161 attaatatat ctaaaatttg ggattagtgt agaatgtgta caatatatgg ttttaattcc
    50221 atttttaata attatatcca ttgtagatta ctacacaata tatgtatatg atataacaat
    50281 agttagtggt ataatagttc aaggtataat ttttttggtc tttttcaata tagttcaatt
    50341 tgattatata aatcatatat ttggactatt gataggcttt attatacctt atattattgc
    50401 taaactaaca aaaggattag gctatggaga tgtaggtctc tatagcttat gttgctttgc
    50461 attgggaaat aattatgggt tttatgtgat agttttatca tttattttag cgtttttgtt
    50521 ttttattatt ttatacataa ctaaaatatt taaagatgta gatataagaa agcaaattcc
    50581 ttttacacct tttatttcct tgtcaacaat tttaataata tttacagagt ataatatatt
    50641 taatttttat tataatttgt tatacaaatt tatataacag gaggtaagaa atgtacgtag
    50701 tagtagggtt aggtaatcca ggaaagaagt atgagaagac tagacataac gttggatttg
    50761 atgttataga tattcttgct aaagaataca atattagtgt tactaaaata aagcataaag
    50821 ctttgattgg tgaagggaga gtaggtactg aaaaagtatt attagttaaa ccacaaactt
    50881 atatgaattt aagtggagaa acattaatag atatatataa atattataag gttgatttaa
    50941 gtaatatagt agtagtatat gatgatatag atttagaagt tggaaagata cgtataagaa
    51001 aaaaaggaag tggaggaact cataatggca tgaaatctat aactaagtgc ctaggttcaa
    51061 atgactttcc aagagtgaga gtaggtgtat ctaaaccaga agctggtcaa gacttagcag
    51121 actttgtact ttctagattt agaaaagaag aatctgataa cataaatgaa gcattagaaa
    51181 aagcggcaga tgctattgac tcaataataa gagaaaatat agatatgtct atgaacaaat
    51241 ataatggata gtgagaggga gatttaaatg aatgatgttt ttttatatcc tttgcaaaat
    51301 tcaaaagagt ataaagatat aataaactgt ataaaaaata caaaaggctc attattggtt
    51361 aatggacttt taccagtaca aaaacctcat atatcgtatt ctatatttaa tgatttatca
    51421 cgacaaatga tttttataac aagtagtgat ttagaagcaa agaaagtata cgaagattta
    51481 agtttttata tggaagataa agttgaatat ttaggttttc aagatattta tttttatcac
    51541 ttggatgcaa aggacagaaa cgaagaagcg aagaaattaa aagtattgtt aaaactagca
    51601 aataaagaaa aaatcatttt agtaacttct attgaggcag ttttaagaaa atacatacca
    51661 aaacaagttt tgctcgatag tgtatctcat tataaggtgg gagatagctt agacttagaa
    51721 aagttaactg agaaattagt ttcattggga tatgaaagag tttcaaaaat agaggggttt
    51781 ggtcaattta gtataagagg tggaattatt gatgtatttt cacttgaata tacaaatcca
    51841 attcgtatgg aattatttga tgatgagata gattccatac gaacttttga cgtatattca
    51901 caaaaatcaa ttgataaatt gcaacaattc tctataactc catctaggga atttatttat
    51961 cccgaaaaaa ctacagatgc attagttaaa ttaaaaaaag aaacaactaa aaatactgat
    52021 gaaggtacat ttcaaaatat agattatata tcaagtaaga cttattttga aggtgtagag
    52081 aactatatag attatattta tccagaagaa aacaaaagta tatttactta tttagcagat
    52141 gatgcaatag tatttattaa tgatataacg agacttaaag aaagatgcga aaattatata
    52201 aatgagttta gagaaaatta taaattaaat cttgaaagag gtctggctat taaaaaccaa
    52261 ggtaaattgt tatatcacta tacagattta gagtacttag taaaagacaa gtctgtggta
    52321 ttaaactcgc tattgccaaa gtctataaat aacttttcta taaaatctat aataaatttt
    52381 gaaagtagag aagttcctac atttaatgga aaggtagatt tgcttgtaga agaattaaat
    52441 cggctgaaat ataatggata caaaataatt ttagcaacaa ataccttaga aagagcaaat
    52501 aaattaggaa aagatttatt ggacaaaggc ttagaaacga ccatttctaa agatagagat
    52561 attgaaatta aatcttcaca agttataatt gtacctgcac acataaacag tggttttcag
    52621 tataaatcta taaagtttgt tgttataaca gataacgaaa tgataggtgt ttataaaaga
    52681 gcgtctaaga ctagcaataa aaaagttaaa aagggaaaga aaatagaatc ctttttagat
    52741 ttaagtgttg gagattatgt agtccatgaa aatagtggtg taggtagata cacaggaatt
    52801 gaacaaatta cagtcaatgc tattaaaaaa gattatatga aaatagtata tcaaggtgga
    52861 gataatctat atgtgccaat agaccagatg gacaaagtac aaaaatatat tggtgcagaa
    52921 gtagaaaaag ttaagttaaa taagcttgga acaaatgagt ggacgaaagc aaaagcaaaa
    52981 gttaaaaaag aaattgaaga tatgacaaaa gacttgattg agctttatgc aaaacgtgaa
    53041 aaaatacaag ggtataagtt ttcaaaagat acaccttggc aggcagagtt tgaatcgttg
    53101 tttccatatc aagaaacaga agaccaatta aaggctatag aagaaactaa aaaagatatg
    53161 gaatcaagta aggtaatgga taggctagta tgtggagatg ttggttatgg aaaaacagag
    53221 gtagctatta gaagtatttt taaagcttgt atggaccaaa aacaagttgc tgtattggtt
    53281 ccaacaacaa tattggccca acaacattat aatacattca aagagaggtt tgaaaattat
    53341 cctttgagag tagaagtttt aagtaggttt aaaacaccaa aacaacaaaa acagattatt
    53401 gaagatgcta aaaaaggatt agtagatatt ttaattggaa cacatagaat aatatccaaa
    53461 gatataaatt taccaaactt aggtcttgta gtcattgatg aagaacaaag atttggagta
    53521 aaacataaag aagctttaaa gaaaattaaa tctacagtag atgtacttac actttcagca
    53581 actcctatac caagaacatt acatatgtct ttaagtggaa taagagacat gagtgttata
    53641 gaagaacctc cacaagaaag acatcctgta ataacatatg ttacagagag taaggaaagc
    53701 gtaattcaag atgagataga gagagaatta agtagaggtg gacaggtatt ttttgtatac
    53761 aatagagttg aacacattga agaaatggca agtatgattc aaaaactcgt accagatgct
    53821 agagtggctg ttgcacatgg aagaatgact tctaaatcat tagaaaatat aatcctagga
    53881 tttttaaata aagattatga tgtactagtc tgtactacaa taattgaaac tggtatggac
    53941 atatcaaatg caaatacaat gattatatat gatgcagata aaatgggatt ggctcaactt
    54001 taccaattga gaggtagggt tggaagaagt tcaagacaag gatatgcata tcttttgtat
    54061 gaaaaagata aaactttaag tgagatagcg gagaagagat taaaagctat cagagaattt
    54121 acagagtttg gttctggttt taaaatagca atgagagacc ttgaaataag aggagcagga
    54181 aatattttag gttctcaaca acatggtcat atggcagtta taggttatga tttgtacgtt
    54241 aaaatgctta atgatgcaat taaaaaagtc aaaggtgagc ctattgtgga ggaaattgat
    54301 gttgaaatag atttaagtgt aaatgcatac attccagata attatatcaa agatgagttg
    54361 attaaaattg aaatgtataa gaaaatagct tctattgaaa acaaagaaga catgttggat
    54421 atacaagaag aacttgagga tagattctct gatataccta aaccagtcca gacacttctt
    54481 acaatagctt atataaaatc gttatgtaag atattaaaaa tagaaaagat aagacaatta
    54541 aaagatgaaa tattattagt tccaataaca aagtatagaa ctaaacagaa aataggttat
    54601 aatatagtta cagaattaga agaattatta gaaaagatgt gcaaggtaaa ataacttgta
    54661 aatttaagaa gggatgtttt aaatttgaaa aaagtaataa cattggtaat agctatgatt
    54721 ttggtagtat ctgtgactgc ttgcagttct agtaaaggag aaactgtggc aacagttgaa
    54781 ggtacaaaga tatcatcaga tgaatttaaa aaaactatag ctctttataa agattctatg
    54841 gaacaaactt atggaaaaga tatatgggat aaggaagttg aaaagggtgt aaaatacaag
    54901 gacaagttta aagatttaat tcttgaccaa cttataacta cagaagtaat atattctcaa
    54961 gctaaaaaag ataacttact tcctaaaaaa gaggatgttg aaaaaagttt taaagaatta
    55021 aaagacgcta tgggtaaaga tgaaaaatat aaagaacaac ttaagaaatt aggtatagat
    55081 gatgagtttt taagagacca acaagagaaa gatttagcaa tgcaaaacta tcaaagtaac
    55141 tttgctaaaa agactaaaat atcagatgaa gaaatgaaaa aatactatga tacacataaa
    55201 gatgaattta aaaaagatga ggtagaagct tctcatattt tattgaaaac agtagatgat
    55261 aataacaagc cactttcaga taaggaaaaa gctgaagcta agaaaaaggc tgaagaggct
    55321 cttaaagaag ttaaatcagg tgaggatttt gcaaaagtag caaaaaaata ttcacaagat
    55381 acttcagcaa gtgatggtgg taaattagga ttcttttcaa ggggtcaaat ggttgctgaa
    55441 tttgaagatg ctgctttttc tatgaaaaag ggtgaagtat ctgatttagt tgaaactcaa
    55501 tatggatacc acataattaa agtgacagac agaataaatg agcaaacatc atttgaagat
    55561 gctaaagaaa ctataaaaga ccaactgttg aaaaataaat atcaagaaca aatagaaaaa
    55621 ttaactaaag aagctaaggt agaaaaagat gaaaaagtaa taaataaaat cacaatctaa
    55681 gacttaaaaa aagagtaata tgcttaaaaa agctatatct ggataagata tagctttttt
    55741 tgtatatttt tcttaacaat ggtaaaaata aacttaaagt aatttatgga gggattaaaa
    55801 aatatgaaag caacaggtat agttagaaga atagatgatc ttggaagggt agttatccca
    55861 aaagagataa gaaagacatt aagaattaga gaaggagacc ctttagagat attcacagca
    55921 aaagatggag aagtcatact taaaaaatac tctccaatag gtgaattaaa tgaattttcg
    55981 caagaatatg ctgaaacatt agctgaagta ctaggatatg gagtgttgat aacagactta
    56041 gattctataa tagctgtgtc taagcttcct aaaaaagatt ataaagaaaa aagtataagt
    56101 gatgagttag aagcaataat aagtgaaaga acagtaaaat attcaaaaga taaaaacatt
    56161 ttaccattat tcaaggaaga ccaaaaagaa tatactagtc aagttatcat gcctataatt
    56221 agtgcatctg gtgactgtat aggttcaatt gtagttgttt caaaggataa agatacaatt
    56281 gcagaaagtg aagaaaaatc tttaaaaatt gctgctaact ttttaggaaa acaagttcaa
    56341 taattttatc ataaagacct tttacatgaa ggtcttttga agtttttgtg taaaatattg
    56401 aatatagcat taaaaatatg gtatattaaa gtttgttata taaacagtat ggaggttatg
    56461 ttatgaacaa taacaaaaat aaagactcct ttctaaaagg agcccttata cttgggctag
    56521 caggtgtaat cgtaaaaata atgggtggat tttttaggat tccccttgga aacatgattg
    56581 gttcagaagg aatgggatac tatcaggctg cttatcctgt gtatactcta tttttaacat
    56641 tagctactgc tggatttcca acagctgtgg caaaattggt ttcggaaaaa gttgcaattg
    56701 gaaattttaa aggtgcaaat aagatattta aggtatcaca tacagtttta ttcattaccg
    56761 gtataatatc attttgtata ttattttttg gagccgacta catagtaaca aacgtaatga
    56821 aaaatccagg tgcgttatac tctatgaaag ccattgctcc agcattacta tttgttcctg
    56881 caatgtctgc atatagagga tattttcaag ggagacaaga tatgactaag attgctgtgt
    56941 ctcaagttgc agagcaattt tttagagttg tattaggtct tactttggct tacttgctta
    57001 tgaagagtct aggtcagact tttggtgcag caggtgctat atcaggggct acaataggtt
    57061 caatagcatc tatgctttat ttggtgtttg cttatatgct agggagaaaa gagcgtagag
    57121 cagagataga tgcaagccaa agatttaaag atgaaagagt gtcttatatt tttaaaaagc
    57181 ttttaactgt tgccattcct ataacaatag gagcatcagt aatgccactt gtaaatatga
    57241 tagataatgt tattgtaata agaagactta tggaagcagg ctttacatac aaagtggcaa
    57301 attccatgtt tggtcaatta acaggtatgg caatggccac aataaattta ccagcagtca
    57361 taacaactgc aatgagtatg agcttagttc cagcaatttc taaagcatat gcattaggaa
    57421 ataagtcaaa agctagaaaa gatactaaga gtgcagttaa ggtaacttta cttatagtat
    57481 tgccttgtgc gtttggtatg gcttctttag caataccaat aatgggtctt ttatttccac
    57541 atgaaccatc ttctgtagga actattttgt ttactcttac tccatgtgta ttatttttag
    57601 gattaataca gacattgact ggtataattc agggaatggg aaaaccaata gtacctgtaa
    57661 ttgctttatg tgttggtatg ctatgtaaaa ttgtaataag ttatacttta acaggtatac
    57721 cagatattaa tgtactgggt tcagcatttg gtacagttac agcatacttt gtggcagcca
    57781 tgattaactt actatatgtt aaaaaacaca tgaatgtaaa cttttctaaa aaagaattta
    57841 taataaagcc atttataaca gttatgacaa tgtttataat ggttaagttg agttatggtg
    57901 ctttagttgg attcttagga aacagcatat ctacaataat tgcaatatgt gttggtggaa
    57961 ttgtatatgt agtagtaata cttggaatag gtggaattaa aaaagaagag attctaacta
    58021 tgccaaaagg tgataaatta tataaattgt taaaaaaagt taaacttata agataatata
    58081 ggaatatata tgcaaataaa ggaaatacta tacaaatagt aaatctcctt atttgtataa
    58141 taatataaaa ataaagtgaa atttgctaat aggagtgatg ttatgggtaa gataagtatt
    58201 gtaggtcttg gaccaggaga ctattctctt ataagccaag gtgtgttaga atctttaata
    58261 aatagcccta aagttttttt tagaacagag aaacatccaa taattgataa acttaaagaa
    58321 actatacaat acacttcttt agactacttt tatgaaaaag aggaaaattt tgataatgtt
    58381 tatttgaaaa tatctgaatt tatcatagaa gaatcagcaa agggtgacct agtgtatgct
    58441 gttcctggtc atccaagagt tgcagagaag actgtaagta taatagagag attagccaat
    58501 gaaaagcaca tagcagtaga aatattgcct tctatgagct ttgtagacgc tatgtttaac
    58561 tatttggcta tagacccatc tgagggattt aaactggtag atgcttttga aatagaaaat
    58621 tcatatatag acttggatac aagtatgata ataactcagg tatatgataa atttatagca
    58681 tctaatgtaa agttaaaact tatggaacat tatgactata atcaagaagt atgtatagta
    58741 aatggagctg gtgtcaaaga tttagaaagt aaaaagttta taaagctttg tgaattagac
    58801 agaaatgaaa acttttttga ctatttaact agcttatata tacccaaaag ttctaaaaag
    58861 atgtataata cagtacatga cttagaaata atagttaata cacttagaag tccatctgga
    58921 tgtgaatggg ataaaaaaca aacacatcaa tcattaaaaa attctgttat agaggaagca
    58981 tatgaacttt gtaatgcaat tgataataat gatatagatg aaatggtaga agagttaggt
    59041 gatgttcttt tacaggttat attccattgt cagataggaa atgaggaggg ctactttgat
    59101 ttaaaagaag tagtaaatgg catatgtaaa aaattaattc atagacatcc tcatatattt
    59161 agtggtgcaa aattagatat gtctaagttt gagaaaactt gggaagaatt aaagaaagaa
    59221 gagaaaggcg aaagtactat aacagatggc cttagaagga taccaaaaca tttaccagct
    59281 cttatgaagg ctagtaaagt tcaacataaa gcagcacttg tcggctttga ttgggacaat
    59341 gtagaagatg tatttaaaaa aattgaagaa gaatatcagg aattacttga cgaacacagg
    59401 gtcgggaata taaaatacat aaaggaagag ctaggcgatt tattattttc gatagtaaat
    59461 ctagctagat ttttagacat agactcagga gaagccttga actgtactac agaaaaattc
    59521 ataaatagat ttgactttat agaaaagagt gcaatgaact tgaataaaaa attagaagac
    59581 atgacacttg aagagatgga tgagttttgg aatcaggcta aaaaacaata aaattgtgga
    59641 aggaaatttg tcaaaaatct agaactttta tcacaaagat agaagtctat attataaaca
    59701 tttttaggag gtaaatatcg tgaataaagc tgaattagta tcaaagatgg cagaaaaaag
    59761 tggattaaca aagaaggaag cagaagctgc gttaaacgca tttatgagtt ctgttcaaga
    59821 tgcactagta aataatgaaa aagttcaatt agttggattt ggaacatttg agacaagaga
    59881 aagagctgct agacaaggaa gaaatccaag agatccagag caagttatag atataccagc
    59941 ttctaaagca ccagttttca aagctggaaa aggattaaag gatataataa atggataatt
    60001 agaaatcttg tgaaaaagtg tggataaatt tgccacactt tttctataga accaaggaag
    60061 gtgataatta tgagattaga taaattttta aaagtatcta ggattataaa aagaagaact
    60121 gttgctaaag atgcatgtga taaagggatt gtcactataa atggaaaagt tgctaaatct
    60181 tcttcagagg tgaatattgg agatacatta gaaatacaat ttggagagaa gaagatgaag
    60241 tttaagataa atgaagtaaa agagcatgta ttaaaaaatg atgcaaaaga aatgtacgaa
    60301 attttagaat aaaattaata gtcctctagt atattaatag tagtattatt gaaatattta
    60361 ctggggggat attatggaac agaacataac tttgaaagat agatcaaagc tggttatttc
    60421 gggagtcgag cacatatact cttttaatga caaaagagta gagctcaaaa cttctgtagg
    60481 cgagatggta attgaaggcg aaaacctaga tatgagcaag ctaagtatag acgaaaatat
    60541 aattagcata gatggaacta taaattctat ggtatatgca aagcctaaaa agccacaaga
    60601 gagtttcttg aaaaaggtat ttagatagat gatacctttt actcaagatg taagcatttt
    60661 ttatgctact atttatggtg gaatactcat aggggtgttg tttgattttt atagagggct
    60721 aagagggaat tttaaattca tcaattattt tgctataata tttgatgtat tgttttggtt
    60781 tctagctacg gttataatat tcgtaacaat aaaccttaca gaattttttg atttaagata
    60841 ttatcatttt gtggctttgt ttattggttt tattttatac tataacacca taagtaaaat
    60901 tgttttatca attatcaata aaataattcg ttttgtgaga aattctttta aaaaagttac
    60961 acattacata gtcagttttt taaataattt gtattacgtt ataatctatt cactacacct
    61021 actatttgat attatatttt atataccaaa catatttata gcaacaagaa aaagtataaa
    61081 aagaaaatca aataaaaaat taaagaacaa gaaaaaatca aaacctaaga agaaaaaaaa
    61141 taaaactaaa aaaagggtgt aggaaaaatg aacttaagaa aaaaattttc aggacagatt
    61201 attgttatca gtttattttt aggaatatca atttttagta tgatgacagg atttgtattt
    61261 gagtatacaa aagctaagga atataaaaaa gaaatagcaa gtctaaataa acaattaaaa
    61321 aagactgaaa tacaaataaa cagcttaaag aaagatgaaa aatcatatga aggagactta
    61381 gaggatatag caagaaaaag attgaatatg gtaaaaccaa atgaaacagt ttatgtagac
    61441 ataaatcgtt gattaaaact tgctaataag tttacagatg tattgatttt aaaatttaag
    61501 atttaataaa aaagtcattc agttaaggat ggctttttgt ttttattgag gagggaattt
    61561 cataatgaaa gtaggagcta tagatattgg gacaaactca atgagattat tgatagcaga
    61621 ttatagagat gggaatttta taaacagaaa aaaatttgtt aatacaacta gaattggtta
    61681 tggtgtggat agtaaaggat atataaaaga agatgctatc aaaagaaata taaaagcatt
    61741 aaaagaattt tctgataagt gtaatgatga aaagtgtgaa aaaatttatt gtatggggac
    61801 atctgctctt agagacagta aaaatggaga ttactttgtt aaattagcta aagaagaaac
    61861 tggaatagat gttgatatag ttacgggaat agaagaatca aatttgggct ttaagggtgt
    61921 tttagaagga atacaaggtg atgatactat tattgttata gatattggag gaggttcaac
    61981 agaatttatt gtaggtgatt cagaaggaat tatatttaat aaaagtgaga atgtaggtgc
    62041 tcttaggatg acagagaggt ttttgaagca cgatattatt gaagatgaag agtttgaagc
    62101 tatgagttca tttatatatg gtgagattga taaaacgtta aatcatataa aagacaaagg
    62161 attaaaaact cttataggta taggaggtac gattacatca ttatcagcaa tgaatcaaga
    62221 attagagatt tactctatgg aaaaaataca taatagtaaa atacacaaaa aagaaataga
    62281 agaaattcta caaaaattaa taaagatgac attaagtgat aaaaagaact taaaagggct
    62341 acaagaaaaa cgtgcggata ttataactgc tggtgtaaaa atattgaata taataatgga
    62401 aaaattagaa atagaagaaa taaccgtaag tgaatacgat aatttggaag gcttaatatg
    62461 tcaaaagtca aaaaaaatgt attaatattt tttgattttt aaaaccgatt atgacaaaaa
    62521 aaattactcc tctttgttac aattcttaat ataaacaaaa gaggaggttt ttttatgcaa
    62581 agaagtgtag cagttgtgaa gaataagact gtaaaaccaa atgtcataaa attaataagc
    62641 ggatatattg attttactac ggcggcattc gctgtgatgg gatttttact tagtaggtct
    62701 attatagtag actctgtagc tcctttagga attgcctttt ttatatgtgc atcaaagatt
    62761 gataagtaca agatacctgt attcatatca acattattag gaatattgtt atcatctaat
    62821 gattttggaa atgtagcaaa atatgctgtg tgccttacaa tatttatggc tatgaataaa
    62881 aaattaaaat tatcagattc aattgctaag gttgcattta taggtgcaat aatactactt
    62941 ccaatctcca tgggacaagc tttcttttca aataggtatg tatatggtct tcttatagca
    63001 ggagtagaag caataataat gtttatatct atatatgtgt tttcttatgg gataggttta
    63061 atggccaatg tcaacaatag gatgtctgta cggacagaag aagccatatc aataagttta
    63121 ctgctaacct ttagcataat gggaatagga gatatatctc tgtttggaat atcaataagg
    63181 accattttat cgaccgtatt gatattgctg acctcaatag taggtggagc tactatggga
    63241 gcatctagtg gtgtaattat tggtgtagca tccattttaa ataatatgac atcagctgtt
    63301 tatatgggga tatattcttt ttcaggatta ataagtggag catttaataa aataaataaa
    63361 tatttctgta tactaggata tattctaagt tggacaataa tatacctata tacttctggt
    63421 attacttcta atatgatgca attaagagat atactattag gttgtttgat agtactagta
    63481 ttgccagagc gattatttaa taagattgaa aaattaataa aaagtaatgt ggcttcaaat
    63541 gaaatcgtat acgattatat tatgagaagc aaaaacctaa ctaatagcag attgaacagt
    63601 atttataaaa cttatgatga ccttgcagat acatttgata agataagaga aaaagacaaa
    63661 gtgctagacc aaagagacat agctaatgta atagatatga ttcataatga tgaatgtaaa
    63721 agttgtagta tgagaagaat gtgctgggag tcaagattta atcatacata cactatgata
    63781 tataacattc ttgaaaaaat tgaggagaaa ggggagttaa gtctaaacga tatacctaaa
    63841 aattttagaa aggaatgtat gaaaccagag tcaatagtga aaatatcaaa tcactattac
    63901 aaaatgtttg tattagatta tgattggagt gtgaagtttt cagaaagtag aaagttaata
    63961 gcaaatcaga taagaagtat ttcaaaatcc attaaatcct tatctcaaga tttagaaggc
    64021 gacataatgc tagatataga aaaggaaaaa aatatatatg aacagttaga aagatatgat
    64081 ataacagttg ataaagtaag ttacttaaca aaaagtaaca gtgagtttga aatatcaata
    64141 gaaaagaaaa cttgccatga tggatgtatg tgtgaagata aaattgtaaa tataatttca
    64201 gacctagtag gggaaaatat gtctgttaga aaaataggat gtcactgttt aggaggtaaa
    64261 tgcaaggcaa cattcgttaa atctcaaaaa tataaggctg ttacagaagt ttcagcaatg
    64321 tcaagggatg ggcatatcct ttgtggagac aattacacat acatggagat aaatgatggt
    64381 aaatacatga tggctataag tgatggtatg ggaaaaggta aaaaagcata tgaagaatct
    64441 tctgccacta ttgatatact ggaaaaaatg atagatgcaa aaattaaaga tgagatagtc
    64501 attgatacta taaataatat gttgttgtta aagtcttcag aagagatgtt ttcaactctg
    64561 gatttgggaa ttttagattt aaaacgaggt tgtttagaaa ctataaagat gggagcgtgt
    64621 tctacttata taaaaagaga ggatggagaa gttgacttaa tatcgtcatc atcattacct
    64681 gtgggtatat tatcagatgt aaaaattgat agaaaaaatg taaaagttaa ggaaggagac
    64741 tatgttataa tggtttctga tggaattgta gatgcaggaa gaaataacaa tttaggagac
    64801 aattggttaa tatacttctt aaagaatata gagactacaa atccaaagga aatttcaaat
    64861 ttaatattgg atagagcatt agagttacaa gcgttacaaa ttgaagatga catgactgtt
    64921 ttagttacga aaatatgtac aaaataaggt ttacaagatt atgtgtataa gatgtggata
    64981 acttgtgtat aatatgttaa tagacttttg aaaaaagtat agttattaac aaaaataaca
    65041 tataaatcaa caaaatttgt tgataagtat ggcagaaatg ttaataaaat gtaaaaaaag
    65101 aaaaaacaaa agctattatc aaaagagatt catgttgaaa taactaaatt tctaagtaaa
    65161 gaaaacctgt gaatatgttg attattatgt ggataactat taattcgata tttatttata
    65221 tagtatgaat aattgaataa tatatctaaa aaaagccatc ctattattaa gtaaacttat
    65281 tgaaaggatg gctttttatg aatagaaaat tgtgcaaagc aataatacct ttatttattt
    65341 ctattttttg catgttaata ataagtataa aagctgacca aggatatcaa aagaataatg
    65401 atagagcaat tactgtcatg aataataatg atactgaaga tgatgctgaa aaaaaacaag
    65461 aaatagacta tgaaaaaaca tgttggaatt tgcaatcttt gtttaaaagt gatgaccaat
    65521 ggaaaaaaga acttaagagt tttgataaag atactaaaga gttgaaaaat tacataggta
    65581 aagtaacaaa atcgccaaca catttgtctt ttgccttaaa cattaaagaa aaacttgata
    65641 taagactaaa taaactttct gcctatgtaa aattaaaaca agatactaat aaaaattcgt
    65701 ataagtactt agatatgaat gatagtatga gtaaatccta tgggaattat atggggattt
    65761 gttcagattt agagttagaa atactaaaat tatcaaacaa agattacaag aaaattattt
    65821 caaataaaaa tataaatagg aaatatggtg catatttaag agatataaga aggaataaag
    65881 tacattattt ggatgataaa tcagaagata ttctaagcaa agtatcttca ataagtagtc
    65941 ttccaagcca agtatatgaa ttatttagaa atatggatag aaaaactaat ttgacaccag
    66001 ctcaatatgc gagtgaatta gagtcatcag atagagaaaa tagaaaaaaa gcttatcaag
    66061 atgaacttat aacttacaat gataatataa atacaattgc aggtttaatt actggtcaag
    66121 ttaaaaagaa tattttttat tctcaggaaa gaggttatga gtcttcttta gaaatgtacc
    66181 tggaatctga taatgtagat gaaaaagtat acaataattt gataaggact gtaaatagta
    66241 atatggacag tctacacaaa tatatagatt taagaaaaaa agtattaaaa ttagataagg
    66301 ttcattcata tgatatgttt gtgcctatgg ttaaggctgt tgataataat attaattatg
    66361 aaaaggcaca aagtatagta tattcagcgt tgtcaccttt aggaaaagaa tacaatgatg
    66421 tagtatataa agctttcaat gaaaaatgga tagatgttta ttcacatgaa aataaagtaa
    66481 gtggtggata ctgcttatct gtttatgaaa atcatccata tatactttta aattataata
    66541 attcattagg ttcagtttcg actttatcac atgagcttgg acatgctgta tatgaatatt
    66601 taagttctaa gaatcagaat tattttaatt caaatccgtc tatatttact cacgaagttg
    66661 catctacaac gaatgaagca ttactttacg agatgcttat aaaagaagct aaaggcaatg
    66721 atgaaaaagc ttattatata acacagtatt tagacttgat aaaagatact ctttatacac
    66781 aaactatgta tgctgaattt gaaaaaacta tacacgagtt agtagaaaaa ggaaaaagtg
    66841 taaacgcatt agtattaaat gatgtttggg gacaacttct taaaaaatat tatggacaaa
    66901 gctatgaatt agaccaatta tctaaggtcg gatgggctag aatcccacat ttttataata
    66961 gtttctatgt ctataaatat gctacaggtt gtagtgcagg agtaagtttt gcccaagaca
    67021 tcctaaagaa tggttcagat aactatatga gctttcttaa aaagggaagt tcagattatc
    67081 caataaactt gttaaaatca agtggcataa atttaactaa tacaaaaccc atagaaaata
    67141 caatcaaaaa atttgatagt ttagtagcag aacttgaaaa gctattataa acattattgt
    67201 aattcacatg gtaaatatat cgaattaatg ataaaatata ctaaaagttt aaagtcctat
    67261 caagatagga ctttttattt attaattagt gggagatgat tctatgcaag ttaaagttaa
    67321 aaaggctgta ataccagcag caggtttagg aactagattt ttgccagcta caaaggcaca
    67381 accaaaagag atgttaccaa tagttgataa gccaactcta caatacataa tagaagaagc
    67441 tgtggcctca ggaatagagg aaatactaat aataacaggt agaaataaaa aatctattga
    67501 agaccacttt gataaatctg tagaattgga acttgattta gagaaaaaag gtaagaaaga
    67561 attgttggag gtagtacaaa acatatctaa catgattaac atacattata taagacaaaa
    67621 agagccaaaa gggttgggag atgcaattta ttgtgcgagg cattttatag gtgatgagcc
    67681 gtttgcagta atgttagggg atgatattgt agataatgat gtaccttgtc taaaacaact
    67741 aacagatgca tatgaggaat atagaactac aatattgggt gttcaaaagg taaatcaaga
    67801 agatactaat aaatatggga taatagaagc aaaaaatata gaaggtagag tttacaaggt
    67861 aaaagacatg gtagaaaagc cagagtcagg aaaagctcct tcaaatattg ctattttagg
    67921 tagatatata ataactcctg aaatctttga tattcttaag gatttaccac caggtaaagg
    67981 tggagaggta caacttacag atgctttaaa aatattatct aagaaagaag ctatgtatgc
    68041 atacaatttt gaaggcaaga gatatgatgt tggggacaag ctaggatttt tagaagctac
    68101 tgttgacttt gctctaaaga aagaagacct taaggaagat tttataaaat atttgaaaca
    68161 tgtatgtagt gaatttgata aaaataacaa tgtgttatac aataatacag atgaaaaaat
    68221 agatttaatt gaagtcaatg aagctagaga acttgaaatt caaaaataat taatgaaagg
    68281 tgatgtatgg tggcaaaatc aagtcagaaa agaattaata gaataatagc tgttgggatt
    68341 gtgggaatcc tcgtaataac gagtataaca gctgttttga gtatgattgc aactatgtaa
    68401 taagataaat gacagcagtt gatatacata aatataaaaa ccatatataa cttgatgtag
    68461 t
//
LOCUS       DAYAPA010000003        64622 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.003, whole genome shotgun sequence.
ACCESSION   DAYAPA010000003 DAYAPA010000000
VERSION     DAYAPA010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 64622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64622
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.003"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(<1..442)
                     /locus_tag="ACGI59_000120"
     CDS             complement(<1..442)
                     /locus_tag="ACGI59_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine rich domain-containing protein"
                     /protein_id="HFG9737934.1"
                     /translation="MQTEWNFGYNGSPQSVILKPGKYKFECWGSSGGINNSSWYTDAK
                     GGYSKGEITLKKQTTLYVYVGESGFASSSTSNNTKSGFNGGGKGYLNQQIMGTYYSMY
                     GGGATDIRLVGGAWDNEQGLLSRIIVAGGGGGSYYPYTGGAGGGL"
     gene            complement(457..1482)
                     /locus_tag="ACGI59_000121"
     CDS             complement(457..1482)
                     /locus_tag="ACGI59_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFG9737935.1"
                     /translation="MAIDKSYYTIITDVGKAKIANASVTGNKVGFVKIQLGDGGGSEY
                     TPTESQTALKNVVWEGNIGNTTTDETAPNCIILESLIPSNVGGFMIREIGYLDDENNL
                     IAISKYKECYKPSIEQGAVVDMKVKTVLIVSNVNNIELKIDPTIIFATLKDIQDLETK
                     IGTVNTKIDTTKIELTSNIETAKTELNTRIDTENEKQNIKIDNIIAGGVNVSHTHIIE
                     VADWTLNNETNMYEVTINHPLLTKRILIALYDEIGEALTPNARAIDDNSILVRNEENI
                     KMYVYLINGNAETHFINATVDDNRVSEMTTYSSKKIEDRFLNLEEKVNGGISSIATSV
                     NELITYC"
     gene            complement(1494..2111)
                     /locus_tag="ACGI59_000122"
     CDS             complement(1494..2111)
                     /locus_tag="ACGI59_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HFG9737936.1"
                     /translation="MDKEINLINYLPQVLQDKEEYIKVFNAENKEIKTLHDKLKDLSN
                     DQFLEDLTISGIKRWEKIMSINPKSNETLEDRRFRIFSKYISKLPYSERFLRNWLDSI
                     VGEGNYELTINNSTYNIHLESDARNQDWFEEVHSFVSSIKPCNMSLDYTRVLVSKDNY
                     MNFGITTLMGQEITIYPWSPPDIETYGEIDILTGNGVGYQEVTIF"
     gene            complement(2104..3153)
                     /locus_tag="ACGI59_000123"
     CDS             complement(2104..3153)
                     /locus_tag="ACGI59_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFG9737937.1"
                     /translation="MFEFMTFENIIKRMLDSVPSTLDKREGSIIYNALAPVAIELSET
                     YIAMDELLDQTFVDTASYYYLEKRCKERGITPLEATNTIAKGVFNIDIPLDSRFNLGE
                     YNYVAIERISECIYKMKCETAGPIFELGQLIPIEYIDKLETAELTEILINGEDEESED
                     SLRQRYYDSLNSQSFGGNMQNYKDEVNKIQDVGGVKVYPVWNGGGTVKLVIINSNFKV
                     PSSDLVNLVQEEIDPLQNQGEGLGLAPIGHRVTVEGVTSTTINISAEITYKSGYTWEN
                     IKLIAEEVIDDYLNELNMSWEDEENLIVRISQIETRLLSIDGVLDITNTMTNDVKSNL
                     TIDSNSIVVRGEVVG"
     gene            complement(3153..3572)
                     /locus_tag="ACGI59_000124"
     CDS             complement(3153..3572)
                     /locus_tag="ACGI59_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HFG9737938.1"
                     /translation="MLPTDNIDYDIEDVSIINFDVRQEPSKTFKLNIEKSKIDGICDD
                     VEALKQTIFLILNTERYQHLIYSWNYGVELNDLIGEPISFVIPELERRIKEALIQDDR
                     IENVDNFEFENIKGKVNCKFMVHTKYGNIKAERVVSV"
     gene            complement(3577..3837)
                     /locus_tag="ACGI59_000125"
     CDS             complement(3577..3837)
                     /locus_tag="ACGI59_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2577 domain-containing protein"
                     /protein_id="HFG9737939.1"
                     /translation="MSQDLLQIIKKAAMDAVETSNPMQIAFGTIESVNPLVVKIEQKA
                     SFEESFLIQTDTFKRYTDKKIGDKLVLIRMQGGQQYLILDRM"
     gene            complement(3851..5824)
                     /locus_tag="ACGI59_000126"
     CDS             complement(3851..5824)
                     /locus_tag="ACGI59_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFG9737940.1"
                     /translation="MADELVLANDRDVRLVIAHWEDFYEPVVLDGITWEIERRGTPSK
                     LEFTIVMDDILEFCEGNSVRLYYKGIGIFYGYIFQKKRDKENHIKIVAYDQLRYFKNK
                     DTYVYSNKTASELVKMLAKDFNLKYNVIEDTKYKISRIEENKTLFDMILTALDDTLRE
                     KKEMYVLYDEFGRITLKNVASMKLDTVMNNDVIEDFDYNSSIDSDTYTKIKLVRDNEE
                     SGKRDVYIAQDSTHMRSWGILQLFDTVDKNMSEAEIKQKCDILLKLYNKKTKSLSLKN
                     VLGDIRVRAGCLVPVFLDLGDIDLQNYMLVEKVKHTFENNSHFMDLTLVDGDEFASYS
                     SSSYSSGNTNNKDEKKNGPAQSTTSKEDNDMINKLNKVFKNKLSNTGSIFVKYSNAYK
                     VNAALMAAISIHETGNGSSSLCKNKNNFFGMKGMSFGSVDEGIKRGISNLSRNYIHMG
                     RKTLESIRDKYAPLYDSPLNKHWVPGVNKFYKQITGNAYSSNSAGTGVRSNEEAEKNL
                     KDITYQVQNSNNANTSTNNNSKVNKVIQEAKNQLGKPYKWGGNGPKSFDCSGLMVWAF
                     KRGAGINLKRVSADQSNDSRGKLLCNINDVKAGDLVFFAYNQGKGNVHHVGLCIGNDK
                     FIHSPNTGDVVKISSLSGRQKQKHDFARARRFF"
     gene            complement(5817..6518)
                     /locus_tag="ACGI59_000127"
     CDS             complement(5817..6518)
                     /locus_tag="ACGI59_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFG9737941.1"
                     /translation="MAYDFYLDGVQLPIPPPKLEIKVTNKNKTVDLINTGEVNILKKE
                     GLSEISFEAEFTHNKLPFYRGTFRDVQFFLSKLELLKTDCKPFQFIVSREMGGKVLFN
                     TNMKVSLEEYNIVEDADNGSDVKVAIKLKQYRDYSTKKLVPATPEKTNYGRTPTPVMK
                     PKEFRPDSSNKPNGKTYTVKAGDSLWSICQKQLGNGSLYKKVYELNKTMMDKANKGKK
                     VPKYTIYKGQVLKLG"
     gene            complement(6534..8876)
                     /locus_tag="ACGI59_000128"
     CDS             complement(6534..8876)
                     /locus_tag="ACGI59_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFG9737942.1"
                     /translation="MATIQTSIRIFDGMTPAFRHMNNAMNIVLSSFEQLQRTSSNAID
                     ANSIRTAREELARAEAGFDRLEQQIRESDNQQRRLNEDINKGASSTDRLVGSAKKLAA
                     TYLGIRTLGGLGNLSDQMTSTNARLSMINDGQLSDGGLNKMIFQSAERSRASYLDTAK
                     IVSRIGMNAGKAFSSTKEIVGFAEQLNKKFVIAGASTEEMNSALLQLTQGLSSGVLRG
                     EELNAVFESAPNIIQSIADYLDVDIGKIRGMASEGMLTADIVKNSLLAAAEQTNAEFE
                     KMPYTLGQIFTSVKNNAVMVFGAIQKKIEDTVSSRGFRTFITDVKDSLYVLGAVGFNV
                     FSGFINLLSSPAFQNFFNVMIVGTSLVVQGLGWIITQALSVANVFSRNWSIISPIILG
                     VVSAISTYIIALGIMQAVTIAGTVANWMSWTSEVIRQRGILGTARALAIGKAEQWGFN
                     AAIYACPIFWIVLGIIAVISAVFILVAVYNHFAGTSISAISLIVGAWYWLCAVVYNVI
                     VGIVNAINICVVEIANIFRTGLYAVQCFFIDMANAGLKAGVNLDKAFDKFATNLANGI
                     IKAVNIAVKGLNWLVQQINKIPGIDLPQMKEFQKVNTVIGTKTTFKPIQKPPEPKAWK
                     PELVEYKNLKSEFIKGYDVGHQLQNKFKDTFDISKIAEKAKKDLGLDDLWDKKYGLGD
                     GLGSAGLNSPLSDAAKGAKDTAGNTAKMAKTMDKSQEDLKYLRDIAEQETINRFTGVN
                     IKIDMNNTNNISKDADVDGIVNVLTEKLNDAMVVSAEGIV"
     gene            complement(8943..9734)
                     /locus_tag="ACGI59_000129"
     CDS             complement(8943..9734)
                     /locus_tag="ACGI59_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HFG9737943.1"
                     /translation="MGLFSKKNKKPCCICGSEKGLMPSIEGENFCTACNCKYIDFSEN
                     ILKVTSIMKMMSNSEGMKKFIEVEKKNLKLLEKFTETKSINSSISFDEEQNLLKISYK
                     NRNQILVEKIIKFDDILEFELLEDGETIVKGGLGRAITGGVLFGGTGAVVGGITGKKT
                     SRKVVEIFKIKITVKDINNPIEYINLINSKVKTNSSIYQKAFSDAQEILSILSIITKY
                     NVMEDKKKSISSSTADEILKYKNLLDMEAITQEEFDAKKKELLNL"
     gene            complement(9996..10436)
                     /locus_tag="ACGI59_000130"
     CDS             complement(9996..10436)
                     /locus_tag="ACGI59_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFG9737944.1"
                     /translation="MGDLNAFLSQNAIKVENRKYVASERFIGEDGKAIEWELKAIDSD
                     RDRQLRKDSTIRVPILNKKGKATGQYTSETDFNTYTLKLCVETIVFPDLHDAELQNSY
                     GVMGAEELLTTMLTPGEYTDLSSEVGEVNGFDRTFEDKVEEAKN"
     gene            complement(10441..10698)
                     /locus_tag="ACGI59_000131"
     CDS             complement(10441..10698)
                     /locus_tag="ACGI59_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9737945.1"
                     /translation="MIKIKDKYEIKDSISFDYSNKRPLEERVSEMYKKAGKYLIDISD
                     KLATDTIDGSSLKPIIIKFEINEAGVATIEKQTKYLVMEVE"
     gene            complement(10751..11221)
                     /locus_tag="ACGI59_000132"
     CDS             complement(10751..11221)
                     /locus_tag="ACGI59_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFG9737946.1"
                     /translation="MAQQIKARDTISASKAECFVTIKGKRYNFMQAINLEAKMEKNKS
                     EIPILGSTTKGNKSTGSKYSGNATFYYNTSIFRELLYEYKETGEDIYFDIQITNEDPT
                     SSVGRQTIILEDCNMDSGIIAKFDADGEYLDEDMDFTFENWKLVEKFNIANGME"
     gene            complement(11243..12553)
                     /locus_tag="ACGI59_000133"
     CDS             complement(11243..12553)
                     /locus_tag="ACGI59_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath family protein"
                     /protein_id="HFG9737947.1"
                     /translation="MALGGGTFVTQNKVLPGSYINFISAKRATSSLSDRGIVAMPLEL
                     DWGIDEEVFQVTSDDFEKYSVKYFGYDYTHEKLKGLRDLFKNIRLGYFYKLNKGVKAS
                     CTIAIAKYSGIRGNDLKIIVTTNIDDNAKFDVVTLLDNKKVDIQVAKVITDLQDNDYI
                     TWKKDATLEATAGLTFTNGTNGEAVTGAEYQAFLDKIESYSFNALGCLATTTEIKSLF
                     VEFTKRMRDKVGAKFQTVLYKKSDADYEGVVSVENKIKDKDLVESSLIYWATGAIAGC
                     DINKSNTNKKYDGEFDVGVNYTQIQLEEALKSGKFIFHKVGDEVHVLEDINTFVSFTD
                     DKNDDFSSNQSVRVLDQIANDIATLFNTKYLGEVPNDKSGRISFWNDVVKHHEQLQNM
                     RAIEDFKADDVSVEPGSDKKTVVVSDAVKVISAMSKLYMTVSVS"
     gene            complement(12554..12739)
                     /locus_tag="ACGI59_000134"
     CDS             complement(12554..12739)
                     /locus_tag="ACGI59_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9737948.1"
                     /translation="MIILSKTLNKEDDYKFTKEQIVNSKKYVNRKDLLNAILKENELY
                     SFSEVEKIINNFMKGVS"
     gene            complement(12723..13160)
                     /locus_tag="ACGI59_000135"
     CDS             complement(12723..13160)
                     /locus_tag="ACGI59_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6838 family protein"
                     /protein_id="HFG9737949.1"
                     /translation="MLNNIIDGISIKLDKSFGNEYTIYSEDVEQGINEPCFFIVPLNP
                     SKVSYPSGRELKKNSFDVHYFPKSNDKSFEINEVAEMLLEELEYIEIDGDLVRGTNMN
                     FEIVDNVLHFFVDYNYFTIKSNDTDKMDTVELFGGLKRGDNFE"
     gene            complement(13153..13578)
                     /locus_tag="ACGI59_000136"
     CDS             complement(13153..13578)
                     /locus_tag="ACGI59_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HFG9737950.1"
                     /translation="MARWGSVDFREFKRACRRMEKFTKIDLDKFCKDAARELAARLLG
                     KVIRRTPVDTGFLREGWSGIAYARSLPVYKSGNNYIIEVINPTEYASYVEFGHRTKNG
                     KGWVKGQHFLTISEMELQGQIDKIIEKKLLILLKGVFDA"
     gene            complement(13578..13922)
                     /locus_tag="ACGI59_000137"
     CDS             complement(13578..13922)
                     /locus_tag="ACGI59_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9737951.1"
                     /translation="MDKTRKAIEMLYRDKCTIVEYQPIKDPVTKRTNNKEVIVLENQP
                     CKLSYKNITSTTDEKVAKLVQTIKLFISPNINIKAGSKLIINDKEYVRSGESAIYPNH
                     QEIILELLKDKA"
     gene            complement(13925..14275)
                     /locus_tag="ACGI59_000138"
     CDS             complement(13925..14275)
                     /locus_tag="ACGI59_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HFG9737952.1"
                     /translation="MEYKIIKIKRLLGLNESDSSKDTILEFILEDVEEIVKNYCNVPT
                     IPEQLNSTILRMAIEMYKNESLGSEDVALGSISSISEGDTSISYRSSASEFKESLLKD
                     YKAQLNRYRKIRWK"
     gene            complement(14278..15336)
                     /locus_tag="ACGI59_000139"
     CDS             complement(14278..15336)
                     /locus_tag="ACGI59_000139"
                     /inference="COORDINATES: protein motif:HMM:NF039452.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFG9737953.1"
                     /translation="MNKNKSLKMNIQLLAGNEGGTKLSDIIVPSLFNPYVINRTMEKS
                     ALLQSGIITNNSEFDKLASQASPLVNMPFFEDLTGESEQIIEDVDLQNNKITSNADVA
                     VIMRRAKMWSATDLSAALAGKDPAGAIAELVSGFWARDMQKELIAILKGVFGTTKAVG
                     ETVAETRLASNILDISSSSGNAAKWSGSAFIDAQQKLGDAKETLTAVAVHSAVEAELR
                     KQNLIETVQPSNSGPIYFYQNKRVIIDDGCPYEGSGSNIVFTSYLFGEGAIALGNGNP
                     VGFVATEIDRDKKKGSGVDYIINRKTFIMHPRGVKFTNENMAKTEGPSRAELSDATNW
                     KPVYEPKQIRMVAFRHKI"
     gene            complement(15353..15943)
                     /locus_tag="ACGI59_000140"
     CDS             complement(15353..15943)
                     /locus_tag="ACGI59_000140"
                     /inference="COORDINATES: protein motif:HMM:NF018515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage scaffolding protein"
                     /protein_id="HFG9737954.1"
                     /translation="MDWLKELLEGIKVEDNKIDVASLQKSIEKKIKETTVTQEDYTNL
                     ETQLNTANETIKKFEGGMTKEDVENLKDKHNNDLEKLKKDMEISKKEYELKGKLKDLG
                     VVDADYIIYKQGGTEKFNFDNEGKVIGLEDTIKVYKENSPHLFTGGKPNYIPKGGDDY
                     KGSNPWLEKSFNLTEQSKIYNDNPALAKELMTAAGK"
     gene            complement(16007..16303)
                     /locus_tag="ACGI59_000141"
     CDS             complement(16007..16303)
                     /locus_tag="ACGI59_000141"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9737955.1"
                     /translation="MFKKDTRGYIKRHIGKPNKSSVRDIISVLIIIVGIALGFIVGLS
                     IIIANVLDVANMIDTNTFTCMKLVFNLAGMIAGYLVGRIICFVLSLTGIFIGSK"
     gene            complement(16322..16810)
                     /locus_tag="ACGI59_000142"
     CDS             complement(16322..16810)
                     /locus_tag="ACGI59_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015981491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9737956.1"
                     /translation="MAKFVKKPVEVEAFKLGYDVEPKWFIENDRVCNFIKEKCINGHV
                     SCDLETLEGTMRANKGDYIIQGVKGEIYPCKADIFEMTYQKVEYRGKNKLSTEMTLDS
                     TNFQENIKNATKELEFFIETLEKADDKIDKLAEKMNKCDCKVDIDKIVKQLGECLRES
                     IE"
     gene            complement(16812..17057)
                     /locus_tag="ACGI59_000143"
     CDS             complement(16812..17057)
                     /locus_tag="ACGI59_000143"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9737957.1"
                     /translation="MLKLYILSIIVFCTGLYLFKMKIDSNEELIEILKNKKVRRKCNF
                     IFMALFPLLNFILGVIFIISSLLVSNEDIIKNLKGDK"
     gene            complement(17128..17376)
                     /locus_tag="ACGI59_000144"
     CDS             complement(17128..17376)
                     /locus_tag="ACGI59_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9737958.1"
                     /translation="MDNKFKNLQHLIDSIDLGLDIEFDLYNKPYNISIGDDDTRFITL
                     CPNGDTKYYKNGKDMVDNYNIDGKLLKDLWKDIQIVNM"
     gene            complement(17378..18913)
                     /locus_tag="ACGI59_000145"
     CDS             complement(17378..18913)
                     /locus_tag="ACGI59_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HFG9737959.1"
                     /translation="MKHNDYWRKRFEQLEEAQNNKSVKYYLELEKQYKLAINSIEKDI
                     LAWYNRFAKNEGISLLEARKLLNTRELEEFKWSVEEYIKYGKENAINQKWMKELENAS
                     ARVHITRLEALKLQIQQQVEVLYGNELDGIDKLMRHIYTSGYYHAAFNVQQGVNVGWS
                     LMNLDTNRINKVISKPWTSDGLNFSERIWGKHRPALINELHTKLTQSIIRGENPKNLV
                     NDFSKRFNVSKSQAKNLIMTESAFFASASRKDCFNDLDVEKYEIIATLDLKTSNICRE
                     LDGKVFDMKDYQVGVTAPPFHCHCRTTTAPWFEDEEGYRAAKGEDGKTYYVPSNMKYN
                     EWYEKHIEGRLGKEKADTLRKMQLNESKDRKQFEEYKKVLGNEIPSKFDEYQHMKYND
                     TIRYEEAKKLYKDVNWQVKNQRNLTSGSVHSVPFESKPNSVFDNYKDGKLIQRRYYGN
                     TGKPRLDLDLTDHRNPKQHKIVPHKHDWLADENNHDKVKREKDMELTKAHKIANKDIL
                     KGE"
     gene            complement(18903..20297)
                     /locus_tag="ACGI59_000146"
     CDS             complement(18903..20297)
                     /locus_tag="ACGI59_000146"
                     /inference="COORDINATES: protein motif:HMM:TIGR01538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0046798 - viral portal complex [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0051027 - DNA transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFG9737960.1"
                     /translation="MELDVIKKLIEQTNSKHSNFVKKADEAEKYYKNENDIIRDRSPN
                     NIGKVNTTNNPLRNADNRIPFNWFGFLVNQKISYLFTYPPTFDVGDDRVNSKITDILG
                     DRYPKEAKTLGKNASIYSKAWLHIWVDDNNNFQYANIDPRQIRAVYSSDLNRKLLAVL
                     REYKKTDDKGKEYVIYEYWTDECCYTYQNKDGNSNINGLEILNKFIEKNLDNKLETQT
                     NVYKHNFGEVPFIEFLNNDLEVRDLDNVKHLIDVYDKVYSGFVNDIEDIQEVIFVLTN
                     YGGADLTEFLKGLKEYKTIDLQSSGADDKSGLSTITINIPIEARDSLLKTTEKQIYVQ
                     GQGVDPKPENFANTSGVALKFLYTLLELKAGLMETEFRLGFAKLVRMICRHLGYSPKR
                     VLQTWTRNMIQNDLELAEICSKSVGIISEKTNLKNHPLVDNAEEEEKQIKKENSQQEY
                     DDLIPNQDGGIDET"
     gene            complement(20302..21531)
                     /locus_tag="ACGI59_000147"
     CDS             complement(20302..21531)
                     /locus_tag="ACGI59_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HFG9737961.1"
                     /translation="MIDELYHSKQLEVLNFALNNDYFMLINYGAKRTGKTIIDNDLFL
                     LELRRVRKIANELGIKLPQYILAGADLGALQRNVLSELTNKYDIEFKFDKHNRFVLFG
                     VQVCCFGHSKTNDLGRIRGMTSFGAYINEGTVANEEVFNEIKSRCSGEGARILVDTNP
                     DQPEHWLKTNFVDKTDGKVIQSFHYKLDDNIFLSERYRANIKKSTPSGVFYDRDINGL
                     WVSADGLVYQDFNKDIHYISKDKLNDINFVRYFAGVDWGYEHFGAIVVIGEDDKGNLY
                     LLEEHSAQHKEIDYWIDKAKSIKEKYGNIKFYCDSARPEHLAAFKRNGIKAFNANKAV
                     LSGVEAVAKRIKTNTLFVVHENVNLFRKEIFMYAWNKNTGEPIKKWDDVLDALRYAIY
                     TDSLGTGIKVLTPNGRR"
     gene            complement(21524..22210)
                     /gene="terS"
                     /locus_tag="ACGI59_000148"
     CDS             complement(21524..22210)
                     /gene="terS"
                     /locus_tag="ACGI59_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit"
                     /protein_id="HFG9737962.1"
                     /translation="MNEKADLAHEDYLKGLKYKEIAEKYNVSLSTVKSWATRYWKQKG
                     CNQSKKVATKKRGAPIGNKNATGPPGNKNAEKFGFFSKYLPEETQDLINEIKDKDKFD
                     ILWEQITIQYAAIIRAQKIMYVKDKEEMVKELKKYESTENGEKIEYEFQFAWDRQASF
                     LNAQSRAMSELRSLIKQYDEMIHKDWNLATEEQRARVEVLKSKINNQESKEDKLDRYF
                     DKLESVIKND"
     gene            complement(22277..22444)
                     /locus_tag="ACGI59_000149"
     CDS             complement(22277..22444)
                     /locus_tag="ACGI59_000149"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9737963.1"
                     /translation="MSDGKICVSCQRTIADVATELTIAYMENASLTPEKIAEIYKLFY
                     KNANEAFNGKF"
     gene            complement(22588..23265)
                     /locus_tag="ACGI59_000150"
     CDS             complement(22588..23265)
                     /locus_tag="ACGI59_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HFG9737964.1"
                     /translation="MLKILQDKNVKVMWSKNGEEVWFNANDVGEELGIVNIRDTLRNI
                     DREYKKKFNESTVGDSYTRNFKDKLPNFGTTFVTEEAVYNMSFRSNKPEAKLFTKWVT
                     QTLKQIRIHGYYIATEKDQEWLDIRAEGKKVRKDFTDEIQEFVYYATSQGSNKPQMYY
                     KHFTELVRKKLGIPKGVKRDELNQSELFDIQALERIISMKLPKLIDKDMNYKEVYKKI
                     KELIEMI"
     gene            complement(23788..24270)
                     /locus_tag="ACGI59_000151"
     CDS             complement(23788..24270)
                     /locus_tag="ACGI59_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9737965.1"
                     /translation="MNKKTLFQEVEGRLYNYKKLESQIRIKDIYIKKLENEFCGCKAQ
                     SYEEKTGPTYNISSSVENEVIKREEDLNRLKEDKKTLEIEKETIECALTSLNSFETEF
                     FNEMYMNNEKINMDYMSNAMHIDRSHCFRIRKRIVCKIIDMLYPKIKEFELPIFSWKA
                     "
     gene            complement(24382..25107)
                     /locus_tag="ACGI59_000152"
     CDS             complement(24382..25107)
                     /locus_tag="ACGI59_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antA/AntB antirepressor family protein"
                     /protein_id="HFG9737966.1"
                     /translation="MNEIMTNENLRVVADDLVTVYETDTGEKIVFARELHNNLEVKRQ
                     FIDWIEDRIKQYGFKENEDYSVFHKNMKNSTGGRPSKEYVLKLDVAKELAMVQNNNKG
                     REIRRYFIRLEKLLNRTLSNSQLSQINTIVNESLLKMEVKHNIQIEQLKKECSEYYRP
                     TSKTKYDISSYIKERLGISKVNEEFELVKKRTLLALGADKWEDIPKDVLLNSLNLIDE
                     SIRIIKSERKTNQISFFEKDNFC"
     gene            complement(25122..25529)
                     /locus_tag="ACGI59_000153"
     CDS             complement(25122..25529)
                     /locus_tag="ACGI59_000153"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0000400 - four-way junction DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFG9737967.1"
                     /translation="MKVFLVIDGEPVGKERPRMNSITKRTYTPNKTKNYEDLIKWLYQ
                     SKVKHYFEGYIKMTLRCYYSIAKSNSKKVKEQKRNNVLRPSKKPDIDNVVKIIADSLN
                     EIAYKDDTQIVEVVASKYYSDKPRVEVILEDII"
     gene            complement(25666..25896)
                     /locus_tag="ACGI59_000154"
     CDS             complement(25666..25896)
                     /locus_tag="ACGI59_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3850 domain-containing protein"
                     /protein_id="HFG9737968.1"
                     /translation="MIHELKILPQYFKEVVNGNKNFEVRKNDRGFKKGDLLVLQEFDG
                     EKYTGLEIRKEITYLLDNSNYLQDGYVVLGIK"
     gene            complement(25919..26188)
                     /locus_tag="ACGI59_000155"
     CDS             complement(25919..26188)
                     /locus_tag="ACGI59_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9737969.1"
                     /translation="MITTQMYKINNEIYLNNICLEENKEITIWVEKNNTHELIWLKIA
                     NVNGKLAIFVQDIENAAIKEWQGRIAYERVISQIEDEQVEKGEID"
     gene            complement(26287..26460)
                     /locus_tag="ACGI59_000156"
     CDS             complement(26287..26460)
                     /locus_tag="ACGI59_000156"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9737970.1"
                     /translation="MNKCEDCKYRAYEEDGEVYFYSDGSYEGGICREEYCSRGYEKKF
                     KNNGENCKSFKKL"
     gene            complement(26557..26952)
                     /locus_tag="ACGI59_000157"
     CDS             complement(26557..26952)
                     /locus_tag="ACGI59_000157"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9737971.1"
                     /translation="MDLPDNVIEIDILRINRNNDKKCKCKNREFIVDTVNKSVHCSEC
                     GFRVEPYDALYELAMNFERVNGQVNTLLKQRKEIASYKPHLIVFKRLESKYRGKKMLP
                     VCPICGEAFYFENLTSWINKDFYERRKNK"
     gene            complement(26955..27674)
                     /locus_tag="ACGI59_000158"
     CDS             complement(26955..27674)
                     /locus_tag="ACGI59_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase family
                     protein"
                     /protein_id="HFG9737972.1"
                     /translation="MKYIASFSGGKDSAAMLLLILEKGLKLDDIVFIDTGLEFKEIYD
                     IIDDFEKRINFKITRIKAEKTFEEYFYTVNEKGKRKGQIWGFPYTLGAWCNSRLKLAP
                     ANKYFNKLGEHKRYIGIAYDEPSRYKRLEKNCIAPLFEEKMTESDCLKYLEEKDFYYE
                     IHHRFKRTGCYLCPKQSLESLKTLRKYYPILWQKMLKFDRDSPVPFRANGVTVNDLEK
                     RFSNEDIENERQISFFSKGVV"
     gene            complement(27679..27918)
                     /locus_tag="ACGI59_000159"
     CDS             complement(27679..27918)
                     /locus_tag="ACGI59_000159"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9737973.1"
                     /translation="MKKYKIKFEEKVTLEHEIIVEIPEEISINDICHCIENKCQRVYD
                     IADYIREFNGRQIDFTEDTCGETEMLVESFRKCKE"
     gene            complement(28014..28262)
                     /locus_tag="ACGI59_000160"
     CDS             complement(28014..28262)
                     /locus_tag="ACGI59_000160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9737974.1"
                     /translation="MDKLYICSSQGVEEVEILEGTKGRFKVGSNSQYLRVINKNVLDV
                     KSASYVASLNRDTAINIWNDEISKEIERLKGFLYTENI"
     gene            complement(28262..28435)
                     /locus_tag="ACGI59_000161"
     CDS             complement(28262..28435)
                     /locus_tag="ACGI59_000161"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9737975.1"
                     /translation="MTNFEMIKSLDEDGMANFFGSTDCICEYCIYEGEPCGYICYESP
                     EGFKEWLYMEVEE"
     gene            complement(28428..28586)
                     /locus_tag="ACGI59_000162"
     CDS             complement(28428..28586)
                     /locus_tag="ACGI59_000162"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9737976.1"
                     /translation="MDDRLEICKKMFPIITKNTRLLCHYCDGRNICSYDEEKAHELLR
                     NEEGAKND"
     gene            complement(28689..29138)
                     /locus_tag="ACGI59_000163"
     CDS             complement(28689..29138)
                     /locus_tag="ACGI59_000163"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9737977.1"
                     /translation="MSLIKYRGYDFENEKWIYSETIKWSDAVDCLFMLKENCEWQKVS
                     NVGVFSKCWSGNNEEIYEGDILKEPYNTKNKYEYGIVRQENYALELYVEWHYLKQFEG
                     KWEEITSKATIMNSKKYVVVGNECENLEEIKKEFLERKEMLENEHLG"
     gene            complement(29125..29256)
                     /locus_tag="ACGI59_000164"
     CDS             complement(29125..29256)
                     /locus_tag="ACGI59_000164"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9737978.1"
                     /translation="MEVSRTEYTIKRAKELYDNGEDIFIAIDKAREEYEEMIKSEFN"
     gene            complement(29256..29621)
                     /locus_tag="ACGI59_000165"
     CDS             complement(29256..29621)
                     /locus_tag="ACGI59_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9737979.1"
                     /translation="MLKVEKYFNGSVADNIFEDDLTLRNYLALYCCVHGVRKNGELVF
                     PTSEKMLIEFNVDKNRKKKKKGSKVKLINAKTGEEKIFDSIDSAACFLRLQSQAVYQT
                     IKKKTKTRSGWKAEYIKEE"
     gene            complement(29635..29892)
                     /locus_tag="ACGI59_000166"
     CDS             complement(29635..29892)
                     /locus_tag="ACGI59_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9737980.1"
                     /translation="MIIIRSQDRLDLMRVNRVEISNKQVCAVFEEESNAREIGRYKSE
                     ERAIEVLNMIQNAVIAGTKFDIINKDGVRCNKEKVFEMPVE"
     gene            complement(29879..30289)
                     /locus_tag="ACGI59_000167"
     CDS             complement(29879..30289)
                     /locus_tag="ACGI59_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HFG9737981.1"
                     /translation="MELKFREWNKNGKEMYSYDEMVCYSKNLLREVYSGVYLPKNNEN
                     FEVMIYTGLKDCDGKEIYEGDIVLCRSIFFTEFAGEVKFKDGCFIAVNEMSGDCFRLS
                     ELAIVKIDGNIYEDLSKLNDIRRCDEGEIRDDNN"
     gene            complement(30364..30771)
                     /locus_tag="ACGI59_000168"
     CDS             complement(30364..30771)
                     /locus_tag="ACGI59_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFG9737982.1"
                     /translation="MNTITLVGRLVADAELKYLPNSGTPKITFSMAVDRRFKDKNGNK
                     ITDFIQCEQLGKHVENLVQYLVKAKPVYAIGELNIYNYKDENGCWKSITKVNVNALEL
                     LSSKNDNNAKQEYVLPGLDPQGFQAIDDDDIPF"
     gene            complement(30787..30960)
                     /locus_tag="ACGI59_000169"
     CDS             complement(30787..30960)
                     /locus_tag="ACGI59_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HFG9737983.1"
                     /translation="MARIYAQRSGSLNEQDRLELLRLLGKAGYTVKIAREKQNSKTTY
                     TYFVEYTEEQEEK"
     gene            complement(31020..31901)
                     /locus_tag="ACGI59_000170"
     CDS             complement(31020..31901)
                     /locus_tag="ACGI59_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFG9737984.1"
                     /translation="MGIIRVSKDKDNPYVVLNKTCLEDVKLSWQAKGLHSYLISKPDH
                     WKIYVNDLYKRSKNGRDATANILRELIENGYITRTPCRDSNTNKMLGGYDYQVYEIPL
                     ENPQKLKSRKTDFPETGFPGNRVSRKPENTEVVSNDFKVNNDITTIVINEQPQQDKTT
                     YIKKYFEKYIGVITPNNFIELMSYLDDGMEADVIIRAIDEAISNGVKNYKYVKTILNN
                     WIEAGVKTNLELTEYQNEFERKKKNKQEKKQSNHKNVNTPNVSKNKFHNFNETFTQYS
                     PDELDDIIKKSQKVKFK"
     gene            complement(31916..32500)
                     /locus_tag="ACGI59_000171"
     CDS             complement(31916..32500)
                     /locus_tag="ACGI59_000171"
                     /inference="COORDINATES: protein motif:HMM:NF016302.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ERF family protein"
                     /protein_id="HFG9737985.1"
                     /translation="MEINNIYIKLMNVRVKFSKLNLKKSGENKFANFKYFELADFLPQ
                     ATGLLEEAKLCPIVTFTNEYATLTLINGENPSEQIVFTSPMRDLQLKGSNELQALGGI
                     ETYQTRYLYIQLLNITESDTFDATSGKNEAKSNSNNRILTDKQLSRLYAIASNANVDK
                     ESLKEKVFKRFGKEIKDLTKQEYDTICNAYENKQ"
     gene            complement(32510..33019)
                     /locus_tag="ACGI59_000172"
     CDS             complement(32510..33019)
                     /locus_tag="ACGI59_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siphovirus Gp157 family protein"
                     /protein_id="HFG9737986.1"
                     /translation="MSTLYELTTDLLEIEEGLTETTGNETEKLEEIKEIIKQEIQNKN
                     TRIVSVILNIDSDINSIDSEIKRMQELKKIKKNALDRLKSNIKECMELLGTKKVETFL
                     GNISIRKSAGSLVIEDEEKIPAIYKTVEQVVKVDKNSIKDFIKKGHEVEGCRIEYGTT
                     LTIPKAKKE"
     gene            complement(33103..33420)
                     /locus_tag="ACGI59_000173"
     CDS             complement(33103..33420)
                     /locus_tag="ACGI59_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9737987.1"
                     /translation="MKSLIIVRNAVEQQLNRANLEINKNEQLYTKLRKKEERNISEEI
                     ELSNALREKSVNERLKIFAESLLEIIDTQIEIKEYEENEDCKIFQLILEELERDRPID
                     VQI"
     gene            complement(33433..33660)
                     /locus_tag="ACGI59_000174"
     CDS             complement(33433..33660)
                     /locus_tag="ACGI59_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9737988.1"
                     /translation="MKILKVKFPTKILKAGKAIRVTCARFGFESDCIITQSQEFELTV
                     VYFDKELDDLVQSSITIDDAISYDYCIEMLN"
     gene            complement(33762..34091)
                     /locus_tag="ACGI59_000175"
     CDS             complement(33762..34091)
                     /locus_tag="ACGI59_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFG9737989.1"
                     /translation="MSVALQFIDTKDLVQELMRRDDTTDIIKMFLDREGIKRMELMTI
                     EEFCEYLKISDVTARNMAREAMITKDFIALKIGRKYMIDRISFEEFIMKNAMKDKDVM
                     KKRKGVI"
     gene            complement(34161..34952)
                     /locus_tag="ACGI59_000176"
     CDS             complement(34161..34952)
                     /locus_tag="ACGI59_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HFG9737990.1"
                     /translation="MNNLQVIERNNERVLTTQQLADVYETDARNISNNFNNNKDRFIE
                     GKHYFLLQGDDLKNFKGIHTEYENLKFTSKMYLWTERGANRHCKILDTDKAWEQFDNL
                     EETYFKVKQHKPTCIEDVLIESLKEMKDLRLQVNQANSIALEAKTEVETIKDVVSLDS
                     NSWRTNTHQLIARIAKKQGGFEHINMLRTESYELLNKRFGVDLHRRLINKRRKMAEEG
                     VSESKREKVNNLDVIQDDKKLIEGYVAIVKDMALKYGISSDLSKN"
     gene            complement(34945..35181)
                     /locus_tag="ACGI59_000177"
     CDS             complement(34945..35181)
                     /locus_tag="ACGI59_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFG9737991.1"
                     /translation="MNKLKDFRIQKGLSRNQLGAELGLTSRYIAFLENGERVPSLNTA
                     VKIAAYFNTSIEYIFLKNNCTKSTFKEKGAEKYE"
     gene            35326..35691
                     /locus_tag="ACGI59_000178"
     CDS             35326..35691
                     /locus_tag="ACGI59_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFG9737992.1"
                     /translation="MIGYRIKELRKEKDITQKELATFLGLTPKMISFYEKEERFPPHD
                     IILKLSDFFNVSTDYLLGKVNVKNIDNLSELELIENLNFSDDIKEALKLISELSPSSQ
                     EKMFKIAKVFLEEELNEKK"
     gene            complement(35706..35846)
                     /locus_tag="ACGI59_000179"
     CDS             complement(35706..35846)
                     /locus_tag="ACGI59_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9737993.1"
                     /translation="MEKSKKLLIDTGDTLNKLRENNSEKLEKYIELLKEYYKQENKKE
                     RE"
     gene            36343..37791
                     /locus_tag="ACGI59_000180"
     CDS             36343..37791
                     /locus_tag="ACGI59_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005634 - nucleus [Evidence IEA];
                     GO_function: GO:0003712 - transcription coregulator
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAP domain-containing protein"
                     /protein_id="HFG9737994.1"
                     /translation="MSILNLFRKNKNKNNVDCNEKSNESKQSYNDSTTLSLECNSNSP
                     HNIEVKNEINKVLNVSCNDTTNLYELEKQAREKSDALYDIHTNSINKFNPQNNILETD
                     TKPLTSIEKSFLKYIIGENIYEPYIATYWTYEYNINYSYLISKFFNMDYLKISNYIED
                     LTKLTVSELKEILKSNNIKSTGKKAELIERIEKEISCKDLSNFFNSSNKYYALTDKGK
                     ELLKDVRKSVTKNTDLEDQCLELIYIDKYEEAYDLICKYESSKNIQRGININWENHKI
                     TPMKIESYKAIKELDINLKDTLLDNIIKSSYILCDMLGNNSKTSILVKRLVGEKIDNI
                     EINNAINSINDVIYNHSTITYNANDSINNKNLHYMDIVKNNKSIPFEVNNKNYNFKIN
                     ENEIYFFNILKIKMYENELKNNFVFDRMSDGAFNVFDTSDNFIGKVKLQGRKKWIMYT
                     KNEFGSEHIYGELIHLIDGIDAWIKYSKEYLK"
     gene            38062..39726
                     /locus_tag="ACGI59_000181"
     CDS             38062..39726
                     /locus_tag="ACGI59_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HFG9737995.1"
                     /translation="MNNENEQLKSDLLINDEKIKNLNFKIPWYYSLWTISILILSTFS
                     TYSISFIVAIIFLFKRNKIMKKHKDSISILLSDVEKINNKYILLNDEIKMKEKHFEDL
                     CESNENKLKELSNLLDKKKVEIDKFDSENQDKFKLIEELKIEKERLDNLIKDKNILKD
                     NINTLNSHLEELKNEREELRDINTTLKNKKEELKRLSEELIQTEDEVLLQSFGLYNPK
                     YDFENSDEYMEKLKEIREMQKLLIRNKTGVKYSDSWTVDGSVQKGRTMTNQNIKTALK
                     LFNSECDIAMSKVSFKNIDSIEKRIRKAFTDTNKLNTSNKVSIKENYLNLKIDELYLY
                     YEYLQMKEEEKEEQRALREQMKEEALVQKEIENQKRKLKKEELQFKNELLRLKSTIPE
                     DENDKLEWEQKINSIEEKLALLSKDLDDVLNREQNTRAGHVYIISNIGSFGENIYKIG
                     VTRRLDPTERINELSSASVPFKYDIHATIFSEDAPKLESALHKAFDNKRVNKVNNRKE
                     FFKVTLDEIRTEVEKNFDKTVEYTKLAEAQEYRQTLKIQELNNKLA"
     gene            40240..41358
                     /locus_tag="ACGI59_000182"
     CDS             40240..41358
                     /locus_tag="ACGI59_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFG9737996.1"
                     /translation="MNIKSAFIRKRGEKFHVYVEYIEETTGKIKQKSYGSYEKKKDAE
                     KHLIEIKSTINNNKFITPNKTTLVERCYKYIMTNEKNWSPYTVINRKSWVKNYIEPFF
                     KDTILIDVNPSLLQIFIDKNFDGSAPSTAKVRYNFLSSVLKEAYRLKEISENPCDFVK
                     LPAQNVTYEIEIYNREETLLLIEKLKDSLIEIPILLMLLLGLRIGEVAGLRWSDVNLD
                     NSIININQILIYANSKITFKEPKTAKSKRALSVPKELVEKLKIEKLKQNKMKLQGTLI
                     NENNLVCLNTNFNAWIPTALSKTFHNFIKRNNLRNIRVHDLRHTNASLLLLGGTNMKV
                     VSERLGHTDIKITMNRYSHVLEEMDKEASDNLSKLLFK"
     gene            complement(41509..42189)
                     /locus_tag="ACGI59_000183"
     CDS             complement(41509..42189)
                     /locus_tag="ACGI59_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFG9737997.1"
                     /translation="MQTKILIIDGDKDNCKKLKGFLEEKGISIDLAYNCEEAIGKIFS
                     NKYDLIFLEIILSDGDGWTLCKKIRNVTTCPIVYMTYINEDQSILNALNSGGDDYLIK
                     PLNLEILYAKVKAILRRMNSYVNNNENNTKLEEYNRTSGVIKLEDKIIKLTPTENKLL
                     NFFIENPEKTLTTKEIYEHVWMNEYLEDNYSVVVAVNGLRKKIEKDYKNPQKIITIRG
                     AGYYFNKE"
     gene            complement(42239..44254)
                     /locus_tag="ACGI59_000184"
     CDS             complement(42239..44254)
                     /locus_tag="ACGI59_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFG9737998.1"
                     /translation="MKKYNILKGTLIVLIVSLLAITGLNLLYKYDNKYTYKSIRASNG
                     ILTIDNDTFTKGKLVFLIDDWEFYNHKIFKPNDFTEQDIKPEYVFIGQYPDFSMRKGT
                     QSPYGNATYRMRIKNEGKDKLLSLELPEIFSASEVWINGEMVSKLGDVGTEKYRPKIK
                     NSVVSFIAKKNTEIIINVSNFSHYYSGLYYPPALGETKDISNMMFYRLLFYSIICFTT
                     FAIAIFSLSVWFLSNKSKIYLYFGCMCVFFAIHVSYPFVHLLGLPLVSFTYAVEDLSY
                     FMVVLCIICINGSISKIEENKVYRFLILPLGITMSIISIVIPIILLPYDMYFVNIYGK
                     LIDIYKYIVFIYILMSSLVSIYKKTCSEHISSYILVSVNTVFGISILFDAVTSNRFEP
                     IFTGWQTEYCGFVIVILFSVMMINRNRIIIKENEKLTRNLEHEVEVRTNELTTLLNER
                     KRFLADVAHDLKAPVSAIQAFIDLIKVGNIHVDEETRTYLYAINQKSNEVQNRVRSLQ
                     EFTSQDKSIGISEKICLNNFIKEIYENNLPDTQACGVNFETYVLEKKIYAKINKEMLT
                     RAFENLIYNALTFTPFDGKIILSMDKEDGFAIIKISDNGKGIPPENIPKVFDRFFTER
                     DNNEPKGQGLGLYIVKSIIREHGGEIFVDSELGKGTVFTIKLSLYFL"
     gene            complement(44326..45003)
                     /locus_tag="ACGI59_000185"
     CDS             complement(44326..45003)
                     /locus_tag="ACGI59_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFG9737999.1"
                     /translation="MYNLLVVDDDVDILNINQIYFSNIGFNVYTSETAKDAMDIVESI
                     VLDCIILDISLPDIDGYKMCNDIKEKVNIPIIFLSNYEHEEEKVRGFLAGGDDYITKP
                     YSLKELELRIYARMRQYKNITSVPNVLKFSALVINVNSRKVTFDGESIDLTTMEFEIL
                     LFLAENKEQVFSKIEIYNQVWKMPDIGDQHTVQVHIAQMRKKINSLSREHQYIQTVWG
                     KGYKFVP"
     regulatory      complement(45583..45666)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            complement(45899..46741)
                     /locus_tag="ACGI59_000186"
     CDS             complement(45899..46741)
                     /locus_tag="ACGI59_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFG9738000.1"
                     /translation="MFKKKIGVLLASTMLVGALAVGCSSNSGGSGDSGDSSKVSVSGS
                     TSIGPLMETIGEKFQEKNQGVSVEVQQIGSSGGIKNAIEGTSEIGMSSRDLKDEEKSA
                     GLKETKIAIDGIALITNTANSVKNLTMDQVKGIYTGKITNWKEVGGNDAPIVIVSREE
                     GSGTRDGFQEIVGFDSEELTKDAQISDGSGNIKTTVEGNENAIGYISFGYVDEKVNSL
                     KINDVEATDANVIANKYPISRPFILVNKEDGLSEKGKALIDYILSDEGQALVSEKGFI
                     KVNQ"
     gene            complement(46993..48777)
                     /gene="pepF"
                     /locus_tag="ACGI59_000187"
     CDS             complement(46993..48777)
                     /gene="pepF"
                     /locus_tag="ACGI59_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HFG9738001.1"
                     /translation="MGTDRSTIEEKYKWKIDKMYSSKEEIEKDISKVKNLIQEVKEYK
                     GKLSESKENLYKVLNISENASRIVQNLYVYTHMKQHEDTRINDNQGRATKTEMLSTDL
                     GVATSYIVPEILAIEENKLSEYLKDEKLSFYAKYIKDILRDKPHTLSEREEEILAATS
                     ELSTIPENVYDMLAYADMEFPEIEDEEGNKVKLSHSNYSLFIKSKNSRVRKDAFEGEF
                     STYEKYKNTFASTLYGGIKSEIFYAKTRKYNSAIEASLFSDDVSLDVYNNLISAIDKS
                     IPNLNKYVELKKKFLGLNEIHMYDLYVPLTDKFDMDIPYDKAQDIILEALKPLGEEYL
                     KVIKSAFDEGWIDVYDNEGKKGGAYSWGSYDSHPYILMSYKNDLNSLFTLIHELGHSV
                     HSHYSRTSQPFLYSDYKIFVAEVASTLNELLLINYLLENSKSKDETIYLLNYYLEQFR
                     TTVHRQTMFAEFEKIVHQRVESGEPLTADEFTNIYYKLNEKYYGKSAIVDKQIGIEWA
                     RIPHFYSNFYVYKYATGFSAASALSQQILSEGSTAVDRYINFLKSGGSEYPLEQLKSA
                     GVDMTKKESIEEALNVFAELVNKLEKEL"
     gene            complement(48843..49508)
                     /locus_tag="ACGI59_000188"
     CDS             complement(48843..49508)
                     /locus_tag="ACGI59_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFG9738002.1"
                     /translation="MIDIGSKEIAIRLLLALIIGGIIGLEREKIRQFAGFRTHILIAI
                     GSCIVSITSIQLFFDYNLYTNADPARMPAQVLSGIGFLGAGAILKNKGGVKGLTTAAG
                     MWATACIGIAVGYGYYELAILGGLFVMIALFVLRIINTFLFSQEKNVLILKLSNLDSI
                     PFLYDKFEKNQLIVRNMEIECRNEEYWKVNFFVSYAKRIHLHDVMKEINMIEGVINVD
                     YLD"
     gene            complement(49659..49820)
                     /locus_tag="ACGI59_000189"
     CDS             complement(49659..49820)
                     /locus_tag="ACGI59_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl-phosphate phosphatase Spo0E family
                     protein"
                     /protein_id="HFG9738003.1"
                     /translation="MANNELEALKNEIEAVREEINTYIEYPEIFQEELVESSKKIDIL
                     INKYMYLSK"
     gene            complement(49898..50407)
                     /locus_tag="ACGI59_000190"
     CDS             complement(49898..50407)
                     /locus_tag="ACGI59_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HFG9738004.1"
                     /translation="MKTRKMTFLGLMIGYSLALYVLETYIPNPLIVIFPGAKLGLSNI
                     ITLISLMLLGVKDTVIIVTIRVILSSIFAGPLSYLLFSIGGAYLSLLFMYLASKIKDL
                     SLIGISIIGAIGHNIGQLIVASLIIENILAIGYLPFMLMASLVTGMFVGLVSRYTIPK
                     LKHFYSKFY"
     gene            50613..50831
                     /locus_tag="ACGI59_000191"
     CDS             50613..50831
                     /locus_tag="ACGI59_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HFG9738005.1"
                     /translation="MTVYDLKIGEKGFINSIDGDIKLSKRLLALGCINSTEIEVKKVA
                     PLGDPIIIRFRGFDLAIRKSDAKNISLQ"
     gene            50849..52795
                     /gene="feoB"
                     /locus_tag="ACGI59_000192"
     CDS             50849..52795
                     /gene="feoB"
                     /locus_tag="ACGI59_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HFG9738006.1"
                     /translation="MINVALLGNPNVGKTTVFNLLTGSNQYVGNWPGVTIEKKEGFLG
                     KEIKVVDLPGIYAMDTFSNEEKVSKSYLENEDVDVIVNVVDASNLSRNLYLTTQLMQF
                     NKPIVILLNMLDIAESKGVNIDAKKLSEELGVIVVPIIAKKKNGVDRIEEEIKGATQK
                     AFIYQKNFGSETETYKKLESILSRCTKTTYTQKKSISDKIDNIVLNPILAYPIFIGVL
                     FLLFKFTFDWVGGPLQEGFASLIEAYISAPVSDMLSNSSPWFKSLIVDGIIGGVGGTL
                     PFFPLIFTLFFGISLFEDSGYMSRTAFLMDKVMRKVGLSGKAFIPIVMGMGCSSPAIM
                     ATRTLESEKDRRVTALIAPLMTCGAKLPIYALFVAIFFPHNAALVTTSLYLVGIVMAI
                     VVALFLNKTAFKTEAEPFILELPEYRIPTIDALIKNTWNKSKGFLIRVVTVMFAMSVV
                     IWGLSSFNVFGYTEDINVSFLATLGHIISPIFKPLGFDDWRTSVAILSGLGAKEIVVN
                     SLNILYGNLTVVLPTIFNGVTAYTFLIFTALYTPCIAALATMKKEYGNKMMFTSFAYQ
                     FILAWIMAFIVKSIGGVLFMGNSIIEALIGGIIIVLALFILFNKFKSTKNGNVCSGCS
                     ICSGCPSANSCSSKEDSNKKNNAH"
     gene            complement(52974..54029)
                     /locus_tag="ACGI59_000193"
     CDS             complement(52974..54029)
                     /locus_tag="ACGI59_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFG9738007.1"
                     /translation="MTIQDYMLETPVRMREIISNADSLFNEVKRTNLKKIIITGSGTS
                     YHSGVQVQPYLQNLLDIDVVKMYPFMITEDTFKFDNENTLVVGVSQGGSSYSTYNAMK
                     LAEDKGCKIASMAGCKNALIDEISDYILTVNCGEEKSGAKTKGYYCTKLNLMLLGLQI
                     AREKGIISSEKYNEEINKILDAINRFEAVYKLSKQWIERNKEKLVNSKEIRIIGHSDI
                     YGDTLEAALKLLETMRIPVTGYEFEEFIHGIYNAINSDSTIFILDTGKEPRVTKMIDV
                     LSGWTENVFAIGRDVTENDKNLKIDITDNPYYQTFNFIVPIQLICGEIPTLRGVDPSV
                     PKDTRFHMKLGSKKLNK"
     gene            complement(54077..54877)
                     /locus_tag="ACGI59_000194"
     CDS             complement(54077..54877)
                     /locus_tag="ACGI59_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFG9738008.1"
                     /translation="MNKLTKKELRSMFWRSFALQGAFNYERMQNLGYCYSMLPAIKKL
                     YNKKENQAKAIERHLEIFNTTTVVVPAILGITAAMEEENANNPEFDESSISAVKTALM
                     GPLAGIGDSLFWGTFRIIAAGVGVSLAKEGNIFGPLLFLLLYNIPAFALRIFGLKYGY
                     QVGVNSLERIQREGLMEKIMSMATTVGLFVVGGMVATMLSITTPLKFNLNGAEVILQD
                     ILDKIIPNMLPLTFAFVIYYMLKRKVSVTKLTIGTIVTGIALHAIGLL"
     gene            complement(54870..55661)
                     /locus_tag="ACGI59_000195"
     CDS             complement(54870..55661)
                     /locus_tag="ACGI59_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFG9738009.1"
                     /translation="MLVQATMLAIIAGVGILDGRIFGQSMLDRPIVTGMLVGLVLGDI
                     KSGIMIGAQLELIWMGIAGIGAATPPDVVTGGVLGTAFAILSGNGAEVALAVAVPVAV
                     LAQSLGVLVRIINSYFSQKAVFYAKQADFKKVTIMMWLPVILFFLSTSVPTFLAIMIG
                     ADKVTALINAIPKVILDGLGVAGTLLPAVGFALLMDMLLSKKMSVFFFIGFLLASYGG
                     LDITAIALFGVCVAFVLNFYINNKENNQNPQLAINTLTEGEIDFE"
     gene            complement(55701..56123)
                     /locus_tag="ACGI59_000196"
     CDS             complement(55701..56123)
                     /locus_tag="ACGI59_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFG9738010.1"
                     /translation="MKKFLIATHGELSRELVDTSKLIIGSLTNIEYFCMTTDKSADDA
                     EKEIRSILSNNKDSEEFVVLTDVFGGSVANICTNLLLQGFKFELLTGVNLPMLLSILL
                     LVEEDTKLIVKSGLEEAKNGIVYVNELLENQNKIKNVM"
     gene            complement(56139..56618)
                     /locus_tag="ACGI59_000197"
     CDS             complement(56139..56618)
                     /locus_tag="ACGI59_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFG9738011.1"
                     /translation="MVSLMRIDDRLIHGQVAYGWATSLGINVILVANDEAKNDQMKAM
                     SLNLAKPSNVTLYIRGVEESGEIVEKFASSKKSNVLVLVKNTKDALVVAKNSGGVLKE
                     INVGGLRYEEGKKKLTDLVAVDDDDINNFKQIGDLGIDIEFRMLPRDKKKQLSDLIK"
     gene            complement(56750..59506)
                     /locus_tag="ACGI59_000198"
     CDS             complement(56750..59506)
                     /locus_tag="ACGI59_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFG9738012.1"
                     /translation="MRDEILSFVNECVLFNNFDELKKESGIITEDIVKKFNLSRTQSS
                     RLLNDLVKSDNLIKINSRPVIFLPKNKINDVVGPTKKSIYENLDELKNECKLLKQETV
                     FNSIIGYDNSLKEVIEQIKTAVLYPNGGLTIMLTGESGVGKTFLAKIIYKYSVSSNVL
                     EKNSPYNVLNCAQYYNNLELLSSLLFGHVKGAYTGANESRAGLIEQSDGGVLFLDEVH
                     RLNSEGQEKLFTFMDTGTFSRIGENGVIRKARVRLIFATTESLSEFLQTFLRRIPINI
                     YVPNIEERGTIEKRKIVEHFIYEESKVLGLPIEITQITLNAILKIKFKGNIGECKNVI
                     KYACGRAYAKNQRRNGNIFVTLQDLPRNLYIENVDIFQFQSGKSKSIIFSPKQFSIEM
                     SNGRNKRNIIYQSFIKCLDYFDRLEKNEITKEKFSKKSSNLMIRLMDNLVFSNNDENN
                     NVLMELIISIMQDIFRNLEVNNNIKYDGNNVLAFSSYLYLKDESYQLSDEYKALENKL
                     LIYLKKESYKEYSIISKISTLITNRLDMYLNNVDIIIMIIFIKSTIIRMKFNQVNATI
                     IAHGYATASSMANVCNRMLGKNIFTAIDMPIDATIADVSKQMSRYLEGDRIKNGLIVL
                     FDMGSLNLIYEDLKNKINSPMLFIDQISTISALEVGNLLMQGKNIEEIAEIMDEETKP
                     NIQLYYPQKNKEYAIISSCFTGIGTAIKIQHLLSESLIGIVDVKIIPYDYDSLVSMGD
                     KEPVFEMYDVMAIVGTANPNVNGVDFILLEDIISGKGEDDVFRIFSRVVENEKIKNIN
                     DSIVKNFSLIRVIDSLTILDSKKIIERIEEGINAIENIQKKKLSNDKKISLYVHLSCM
                     IERLVRQTEIEEYTDMDLLIKNHHSEVKLIKNAFSVLEKSYSVQIPISEIGYIYNIIY
                     GLPQ"
     gene            complement(60104..60907)
                     /gene="proC"
                     /locus_tag="ACGI59_000199"
     CDS             complement(60104..60907)
                     /gene="proC"
                     /locus_tag="ACGI59_000199"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFG9738013.1"
                     /translation="MKTLGFIGSGNMGSAMIGGIVQSGLVKAEDITVSDLSQVALDKV
                     KEKFGVNTTTDSKVAVTNSDVVIVALKPNICEKALTPLKELIDANKIIVSIAAGKTIE
                     SLESFIGSDKKIVRVMPNTPALVGEGMSALCKNSNVTDEELNMIKALFESFGEAEIVS
                     EYLMDTVTGISGSSPAYVFMFIEAMADAAVLAGMPRQQAYKFASQAVMGSAKMVLETG
                     KHPGELKDMVCSPAGTTIEAVKVLEEEGFRAAVIKSIVACIDKSKDMSK"
     gene            complement(61232..63601)
                     /gene="hypD"
                     /locus_tag="ACGI59_000200"
     CDS             complement(61232..63601)
                     /gene="hypD"
                     /locus_tag="ACGI59_000200"
                     /EC_number="4.2.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019471 - 4-hydroxyproline metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-4-hydroxy-L-proline dehydratase"
                     /protein_id="HFG9738014.1"
                     /translation="MARGTFERTKKLREESINAEPHISIERAVLMTEAYKKYEGSVEI
                     PVLRALSFKHYIENRTLSINDGELIVGEKGDSPNGAPTYPEICCHTMEDLEVMHNRDI
                     INFSVSEEARKIHKEEIIPFWKKRQTRDKIINAMTPEWLAAYEAGMFTEFMEQRAPGH
                     TVCGDTIYKKGFLDLKKDIEARLKELDFLNDLDAYNKKADLEAMAIACDAMVILGKRY
                     AEKARQMAEEETDEAKKKDLLLIAETCDVVPAHKPETYHQAIQMYWFVHIGVTTELNI
                     WDAFTPGRLDQHLNPFYERDVENGILDRDRAQELLECLWVKFNNQPAPPKVGITLKES
                     STYTDFANINTGGINPDGQDGVNEVSYIILDVMDEMKLIQPSSNVQISKKTPQKFLKR
                     ACEISRKGWGQPAFYNTEAIVQELMEAGKTIEDARLGGTSGCVETGCFGKEAYVLTGY
                     MNIPKILELTLNNGYDPISKKQIGIETGDPRNFQSYEELFEAFKKQLHYMIDIKIEGN
                     AVIENICAKHMPCPLMSTIVDDCIEKGKDYQRGGARYNTRYIQGVGIGTITDSLTAIK
                     YNVFDKKKFDMDTLLKALDANFEGYEAILNLVSNKTPKYGNDDDYADEIMQEIFNAYY
                     NEVTGRPTVCGGEYRVDMLPTTCHIYFGEIMGASPNGRLCAKPVSEGISPEKGGDTNG
                     PTAVIKSCAKMDHIKTGGTLLNQRFAPSVVQGEKGLDNMANLVRAYFNMDGHHIQFNV
                     FDKNVLLEAQKNPQDYKDLIVRVAGYSDHFNNLSRTLQDEIIGRTEQTF"
     gene            complement(63606..64514)
                     /locus_tag="ACGI59_000201"
     CDS             complement(63606..64514)
                     /locus_tag="ACGI59_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019471 - 4-hydroxyproline metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-4-hydroxy-L-proline dehydratase activase"
                     /protein_id="HFG9738015.1"
                     /translation="MNPLVINLQKCSIHDGPGIRSTVFFKGCPLECVWCHNPESQTYT
                     KQVLYNEERCSKCEACINICPHKAIYKGETKICLDQDKCEFCETCLDYCVNNAREIVG
                     QEYSVRDLVKEIEKDRIFYEESGGGVTLSGGEVMAQDMDFICGVINMCKSKGIHVAID
                     TCGYAKSENYERVAKCADLFLYDIKLIDEDKHIKFTGKSNDLILKNVKILSELGANIN
                     IRIPLIVGVNVDDENLEVKKMIEFLKPLNIQAVSLLPYHNIGKHKYDKIYKKYEGEEL
                     QRPSEEKLEEIKRLFEASNFNTKIGG"
BASE COUNT        20382 a        11600 c         6986 g        25654 t
ORIGIN      
        1 ctaatcctcc tcctgcacca ccagtataag gataatatga acctccgcca ccacctgcga
       61 caattatacg agatagcaaa ccttgctcat tatcccaagc accaccaaca agtcttatat
      121 cggtagcacc accaccgtac atagaataat aagtacccat aatctgttga tttaaataac
      181 ctttaccacc gccattaaaa ccacttttag tgttattact cgtagatgaa gaagcaaaac
      241 cactttcgcc gacgtaaaca tataatgtag tttgtttttt taatgtaatt tcacctttag
      301 aatatccgcc tttagcatca gtataccaag aagaattgtt gatacctcca gaagaacccc
      361 agcattcaaa tttatatttg ccaggtttca atataacact ttgtggcgaa ccattgtacc
      421 caaagttcca ttctgtctgc attttctcac tctcctttaa caataagtta tcaactcatt
      481 tacacttgtt gcaatactag atatcccacc atttactttt tcttctaaat taagaaatct
      541 atcttcaatt ttcttagacg aataagtggt catttcagat actctgttgt catctacagt
      601 tgcatttata aaatgagttt ctgcatttcc atttatcaaa tatacgtaca ttttaatatt
      661 ttcttcattt ctaacaagaa tactattatc atcaatagcc ctagcgtttg gtgtaagtgc
      721 ttcaccaatt tcatcatata aagctattag tattctttta gtcaatagtg gatgatttat
      781 agttacttca tacatattag tttcattatt taaagtccaa tcagccacct ctataatatg
      841 tgtatgagat acatttacac cacctgcaat aatattatca attttaatat tctgtttctc
      901 attttctgtg tcaattctag tgtttaactc tgttttagca gtttctatgt tgcttgttaa
      961 ttctattttg gttgtatcaa ttttagtatt aacagtacct attttagttt ctaagtcttg
     1021 tatatctttg agtgttgcaa agattattgt tgggtcaatt ttaagttcta tattatttac
     1081 attagataca atcagcacag ttttaacctt catgtctact actgcccctt gttctataga
     1141 aggtttataa cattctttat atttagaaat ggcaattaga ttattttcat catctaaata
     1201 ccctatttct cttatcataa atccgcctac attacttggt ataagactct ctagtattat
     1261 acaatttggt gctgtttcat ctgtagttgt attcccaata ttaccttccc aaactacatt
     1321 ttttagagct gtttggcttt ctgttggagt atattcacta cctccaccat caccaagttg
     1381 aatttttaca aatccgactt tattgcctgt aacacttgca tttgctatct ttgcttttcc
     1441 tacatctgta attatagtgt aataactttt atctatagcc aatatatcac ctcctaaaat
     1501 attgttacct cttgatatcc aactccattt cctgttagta tatcaatttc tccataagtt
     1561 tctatatctg gcggactcca agggtatata gttatttctt gacccattag ggttgttatt
     1621 ccaaaattca tgtaattatc tttactaact aacactctag tgtaatcaag actcatgttg
     1681 caaggtttga tgctacttac aaaagaatgt acttcctcaa accaatcttg atttctagca
     1741 tcactttcaa gatgtatgtt ataggtagaa ttatttatag ttaattcata attgccttct
     1801 ccaactatac tatctaacca gttcctaaga aatctctctg aataaggtaa tttacttata
     1861 tatttactaa aaatcctaaa ccttctatcc tctaaagtct catttgactt gggatttata
     1921 gacattattt tttcccatct ttttatacca cttatagtta ggtcctctaa aaactggtca
     1981 tttgataggt cctttaattt atcatgtagt gtttttattt ctttgttttc tgcattaaat
     2041 acttttatat attcttcttt atcctgcaaa acttgtggca agtaatttat tagattaatc
     2101 tctttatcca actacctcac ctctcactac tatgctgtta ctatctattg ttagattaga
     2161 tttaacatca tttgtcattg tgtttgtaat atctaacact ccatctatac ttaacaaacg
     2221 agtttctatc tgagatatac ggactattaa attttcttca tcttcccaac tcatgttaag
     2281 ttcatttaaa tagtcatcaa ttacttcttc tgcaattaat tttatattct cccaagtgta
     2341 accactcttg tatgttatct ctgctgatat atttatagtt gtacttgtaa ctccttcaac
     2401 ggtgactcta tgacctattg gcgctaaacc aaggccttct ccttggtttt gtagagggtc
     2461 aatttcttct tgaactaaat taactaaatc actagatgga actttgaagt tagaattaat
     2521 tattactaac ttaacagtac ctccaccgtt ccaaactgga tatactttaa ctcctccaac
     2581 atcttgtatt ttgttaactt catctttata attttgcata tttccaccaa atgattgtga
     2641 atttaaacta tcataatatc gttgccttag actgtcctct gattcttcat cttctccatt
     2701 tatcagtatt tctgtcagtt ctgctgtttc taatttgtct atatattcga taggtattag
     2761 ttgccccaac tcaaaaatag gtcccgcagt ctcacatttc atcttatata tacattcaga
     2821 tattctctca attgctacat agttatattc tcctagatta aacctagaat caagtggaat
     2881 atctatgtta aaaactcctt ttgcaattgt attagttgct tcgagtggtg taattcctct
     2941 ttccttacat cgtttctcca agtagtaata actggcagta tctacgaatg tttggtctag
     3001 taattcatcc atagcaatat aagtttcaga taactctata gcaacaggag caagagcatt
     3061 atatattata gaaccttctc tcttgtcaag cgtgctaggt acactatcta gcattctttt
     3121 aattatattt tcaaatgtca taaattcaaa cactaaacac tcaccaccct ctctgctttg
     3181 atatttccat atttggtatg gaccataaat ttacaattga cctttccttt tatattttca
     3241 aactcaaaat tatctacatt ttcaatccta tcatcttgaa ttagtgcttc tttgattcgt
     3301 ctttcaagtt cggggattac aaaggatata ggctctccaa taaggtcgtt caactcgact
     3361 ccataattcc aactatatat tagatgttgg tatctctctg tgtttaaaat taaaaagatg
     3421 gtttgtttta atgcttccac atcatcacaa ataccatcta tcttagattt ttctatattt
     3481 aatttaaagg tcttacttgg ttcttgcctt acatcaaaat taattatcga tacatcttct
     3541 atatcatagt caatgttatc tgttggtaac atttcatcac atcctatcta aaatcaaata
     3601 ttgctgtcct ccttgcatcc taattaagac taatttatct cctatctttt tatctgtata
     3661 tcttttaaat gtatctgttt gtattagaaa agattcttca aaagaggctt tttgctctat
     3721 cttaactact aaaggattaa cactttctat agttccaaat gcaatctgca ttgggttgct
     3781 tgtttctact gcatccattg cagctttctt tattatttgt aataaatctt ggctcatatt
     3841 atcacctcac ttaaaagaat cttctagctc ttgcaaaatc atgtttttgt ttttgtctac
     3901 cacttaaact gcttatttta actacatcac cagtatttgg tgaatgtatg aacttgtcat
     3961 ttcctataca tagtccaacg tgatgtacat ttccttttcc ttggttgtat gcaaagaata
     4021 ctaaatcgcc agcttttaca tcatttatgt tgcataatag ttttcctcta ctatcatttg
     4081 attggtctgc tgaaactctt ttgagattta tacctgcacc tcttttgaat gcccacacca
     4141 taagaccact acagtcaaaa ctctttggac cattaccacc ccatttgtaa ggcttgccaa
     4201 gttgattttt tgcttcttga ataactttat ttactttact attattgttt gttgatgtat
     4261 tagcattgtt gctattttga acttgataag ttatatcttt taaattcttt tctgcttcct
     4321 cgttgcttct aacacctgta cctgcactat tagaactata tgcatttccc gttatttgtt
     4381 tataaaactt atttactcct ggtacccaat gtttattaag aggactatca taaagtggag
     4441 catatttgtc tctaatactt tctaatgttt ttcttcccat atggatatag tttcttgata
     4501 aattacttat acctcttttt attccttcat ccacagagcc aaaagacatt cctttcattc
     4561 caaagaaatt atttttattt ttacaaagtg aagaacttcc attaccagtt tcatgtatag
     4621 aaatagcagc cattaaagct gcattgactt tgtaagcatt agaatattta acaaatatac
     4681 ttcctgtatt tgataactta tttttaaata ctttatttaa tttatttatc atatcattgt
     4741 cttctttact tgtagtactt tgtgcaggac catttttctt ttcatcttta ttattagtat
     4801 ttccactact atatgagctt gaagaataag aagcaaattc atctccatca acaagagtca
     4861 aatccataaa gtgcgaatta ttttcaaatg tgtgctttac tttctcaact aacatataat
     4921 tttgtaaatc aatatctcct aaatctaaaa aaacaggtac taaacaacct gctcttactc
     4981 taatatctcc taacacattt tttaaactta atgacttagt tttcttatta tatagtttta
     5041 gaagtatatc acatttttgc tttatctctg cttcactcat gtttttatct actgtatcaa
     5101 atagttgtag tattccccaa cttctcatat gtgtagaatc ttgtgcaata tatacatctc
     5161 tttttcctga ctcctcgttg tctctgacaa gtttaatctt tgtgtaagta tcactatcta
     5221 ttgatgaatt atagtcaaaa tcctcaatta catcattgtt cataaccgta tcaagtttca
     5281 ttgatgcaac attctttaat gttattcttc caaactcatc atataaaaca tacatttcct
     5341 ttttctctct tagagtatca tctagtgctg ttagtatcat gtcaaagagt gttttatttt
     5401 cttctatcct agatatttta tacttagtat cttctatgac attgtatttt aaattaaaat
     5461 ctttagccaa catttttaca agttcacttg cagttttatt actatataca taagtatctt
     5521 tattcttaaa atatcttagc tggtcgtaag caacaatttt aatgtgattt tctttatctc
     5581 ttttcttctg aaatatatat ccatagaaga tacctattcc tttataatac agccttacag
     5641 aatttccttc gcaaaactct aatatatcat ccataactat tgtaaattct aacttagaag
     5701 gtgttcctct tctttctatc tcccatgtga taccatcaag gacaactggt tcgtagaaat
     5761 cttcccaatg tgcaataact agccttacat ccctatcatt tgccagaact aattcatcag
     5821 ccaagtttta acacctgccc tttgtaaatt gtgtatttag gtactttttt acccttgtta
     5881 gctttatcca tcattgtttt atttaactca tatactttct tatataatga accattacca
     5941 agttgcttct ggcagattga ccaaaggcta tcccctgctt ttactgtata tgttttacca
     6001 tttggcttat tggatgaatc tggtctaaat tcttttggtt tcatgactgg agtgggagtc
     6061 ctaccatagt ttgtcttttc aggagttgca ggaactaatt ttttagttga gtaatctcta
     6121 tattgcttta actttattgc aactttaaca tctgaaccat tgtctgcatc ttctactatg
     6181 ttatattctt ctaatgacac tttcatatta gtgttaaata gtacttttcc acccatttcc
     6241 ctcgatacaa taaattgaaa tggcttacaa tcagttttta gtaattctag cttacttaaa
     6301 aagaattgaa catccctaaa agttccacga taaaaaggta atttattatg tgtaaactct
     6361 gcttcaaaac ttatttcaga tagcccttct ttttttaata tgtttacttc tccagtattt
     6421 atcaaatcaa ctgtcttatt tttatttgta actttaatct ctaactttgg tggaggtatt
     6481 ggtaattgta ctccatctaa ataaaaatca taagccacga ttacacctcc tctctaaact
     6541 attccctcag cagatacaac catagcatca ttcagttttt ctgttagtac attaactata
     6601 ccatccacat ctgcatcttt acttatgtta ttagtgttgt tcatgtcaat ttttatattt
     6661 acccctgtga atcggtttat tgtttcttgc tctgcaatgt ctctaagata ttttaagtcc
     6721 tcttgacttt tatccatagt ctttgccatt tttgcagtat tgcctgctgt gtcctttgct
     6781 ccttttgctg cgtcactcaa aggtgaattt aatccagctg aacccaatcc atcaccaagt
     6841 ccgtattttt tatcccaaag gtcatctaga cctaaatctt tttttgcttt ttcggctatc
     6901 ttgctaatgt caaaagtatc tttaaatttg ttttgtaatt gatgtcctac gtcatatcct
     6961 tttataaatt ctgactttaa atttttgtac tctactaatt ctggtttcca tgctttaggc
     7021 tcaggcggtt tttgaatggg tttaaatgtt gtttttgtac caattacagt atttactttt
     7081 tgaaattctt tcatttgtgg taaatcaatt cctggaattt tattaatctg ttgtactaac
     7141 caattcaatc cttttacagc tatattaaca gcttttataa taccatttgc tagattggta
     7201 gcaaatttat caaaagcttt gtctaaatta acacctgcct tgagtcctgc attagccata
     7261 tcaataaaga aacattgcac tgcatataat ccagttctaa agatatttgc tatttcaacc
     7321 acacagatat ttatagcatt tactatccct acaattacgt tgtaaacaac tgcacataac
     7381 caataccaag ctccaactat aagactgatt gcagaaatac ttgtgcctgc aaagtgattg
     7441 taaactgcta ctaatataaa tacagcagat attactgcaa ttatacctaa aactatccaa
     7501 aaaatgggac atgcatatat agcagcatta aatccccatt gttcagcttt gcctatcgct
     7561 aaagctctag ctgttcctaa aattcctctt tgtctaataa cttcagatgt ccacgacatc
     7621 caatttgcta cagttccagc tatagtaact gcttgcataa taccaagagc tattatatag
     7681 gtactaatag ctgaaactac acctaaaatt ataggtgaaa ttatactcca atttcgcgaa
     7741 aatacattag caacactaag tgcttgtgtt attatccaac ctagcccttg tacaactaaa
     7801 ctagttccaa caatcatcac attaaaaaaa ttctgaaaag ctggactact cagtaaatta
     7861 ataaatccac taaatacatt aaacccaact gctccaagta catataatga gtctttaaca
     7921 tcagttatga aagttcgaaa tcccctgctt gaaactgtgt cctcaatttt cttctgtata
     7981 gctccaaata ccataactgc attatttttt acactagtaa agatttgacc tagcgtataa
     8041 ggcatcttct cgaactctgc attagtctgc tctgctgctg caagtaatga gttttttaca
     8101 atatctgctg ttaacattcc ctctgatgcc attcctctta tttttcctat gtccacgtcc
     8161 aaataatctg caatcgattg gatgatgtta ggtgctgact caaacacagc attcagttcc
     8221 tcacctctta atacaccaga actcaaccct tgggttagct gtaacaatgc cgaattcatt
     8281 tcctcagtac ttgctccagc aattacgaac tttttattta gttgctctgc aaaacctaca
     8341 atttcttttg tactgctaaa cgccttacct gcgttcatgc ctattcgtga aactattttt
     8401 gcagtatcta agtaagatgc acgagacctt tcagcagatt ggaaaatcat cttatttaat
     8461 cctccatctg agagttgacc atcatttatc atactaagtc tcgcgttagt acttgtcatc
     8521 tggtcgctta aatttcctag acctcctaac gttcttatac ctaagtaggt tgctgctagc
     8581 ttctttgcac ttccaactaa tctatctgta gaacttgcac ccttatttat atcctcatta
     8641 agccttcgct gttgattatc tgattctctt atttgttgtt ctagtctatc aaagccagct
     8701 tctgcacgtg ctagttcttc tctagctgtt ctaatactat tagcatctat agcattgcta
     8761 gatgttcttt gtaattgctc gaatgaactt aatacaatat tcatagcatt attcatgtgt
     8821 ctaaaagcag gtgtcattcc gtcgaaaatt cggatagatg tttgtatagt tgccattttt
     8881 aacctccttt cttttttaac ataatataag cacttactta tttttaagta agtgcttata
     8941 tattataaat ttagcaattc ttttttctta gcatcaaatt cttcttgtgt aatagcttcc
     9001 atatctaaca aattcttata ttttaatatt tcatcagctg tagaactaga tatagacttt
     9061 tttttgtctt ccattacatt atattttgta attattgata gtattgataa tatttcttga
     9121 gcatcagaaa aagctttctg ataaatagaa gagtttgttt ttactttact attaattaaa
     9181 tttatatatt caattggatt atttatgtcc tttacagtta tttttatttt aaatatttct
     9241 acaacttttc tacttgtttt tttacctgta attcctccaa ctacagcccc tgtacctccg
     9301 aataagactc ctccagttat agcacgacct aaaccacctt taactatagt ttctccatct
     9361 tccaaaagtt caaattctaa tatatcatca aattttatta ttttctctac taatatttga
     9421 tttctatttt tatatgatat ttttaaaaga ttttgctctt catcaaaaga aattgaagaa
     9481 tttatacttt ttgtctcggt gaacttctct aacaatttta aatttttctt ttcaacttct
     9541 ataaactttt tcattccttc tgagtttgac atcattttca taatactagt tacttttaaa
     9601 atattttcag agaaatctat gtacttgcaa ttacaagctg tacaaaaatt ttcaccttct
     9661 atagatggca taagtccttt ttcgcttccg caaatacaac aaggtttctt attcttttta
     9721 ctaaacaatc ccatttattt aatcccccag tacaattttt aaatatatta tactatatta
     9781 gtaaaatttt tacaccagag attatcttct tctacctctc tttgcatctt tgtcagcttt
     9841 tttagcttct tctttttctt cttctacttt gatatctata gaagcagcaa caaatgcttt
     9901 ttcatcaact ggtaaatcca tatattcatg cggtttccat ttaaacttat gaaggcaata
     9961 atgagctata ttagaatcat aatcaccttc ataaattagt tttttgcttc ttctacttta
    10021 tcttcaaaag ttctatcaaa accatttacc tctcctacct cacttgaaag gtctgtatat
    10081 tcgccaggag tcaacattgt tgttaataac tcctctgcac ccattacacc ataactattt
    10141 tgtaattctg catcatgtaa atccggaaat actatagttt ccacacataa tttcaaagtg
    10201 taagtattaa aatctgtttc actagtgtat tgtcctgtag ctttcccttt tttatttaat
    10261 ataggtactc ttatagttga atcttttctt aattgtctgt ccctatctga atctattgct
    10321 ttaagttccc attcgattgc ttttccatct tctcctataa acctttcact tgccacatac
    10381 tttctattct ctacttttat tgcattttga cttaaaaaag cgtttaaatc tcccatattc
    10441 ttattctacc tccatcacca aatattttgt ttgtttttct attgttgcaa caccagcttc
    10501 atttatttca aattttatga ttattggctt taatgacgaa ccatcaattg tatctgttgc
    10561 taacttatct gaaatatcta taagatattt tcctgccttt ttatacatct cgctgacacg
    10621 ttcttccaaa ggtcttttat tgctataatc aaaagaaatt gaatctttta tctcatattt
    10681 atccttaatt ttaatcatct cctattctta taaataaaaa atacacatac ataaatcata
    10741 aatgtgtatt ttactccata ccatttgcta tattaaattt ctcaactaat ttccaattct
    10801 caaaagtaaa atccatatct tcatctaaat actccccatc agcatcaaat ttagcaatta
    10861 tgcctgagtc catattgcaa tcttccagta ttatagtttg acgacccact gaacttgttg
    10921 ggtcttcatt ggtaatttgt atatcgaagt aaatatcctc accagtttct ttatactcat
    10981 acaacaattc tctaaatata gaggtattat aataaaatgt tgcatttcct gaatatttac
    11041 ttcctgttga tttatttcct tttgtagtac tacctaatat aggtatctca ctcttattct
    11101 tttccatttt agcttctaag ttaatagctt gcataaaatt atatctttta ccttttatag
    11161 ttacaaaaca ctctgcctta gatgcactta ttgtatctct tgcttttatt tgttgtgcca
    11221 ttattattct cctttcttat tgttaactaa ctgaaacagt catataaagc ttactcatag
    11281 cactaataac ttttacagca tcacttacta caacagtctt cttgtcgctt ccaggttcta
    11341 cagaaacatc atcagctttg aaatcttcta ttgctctcat attttgcagt tgttcatgat
    11401 gcttaactac atcattccaa aacgagatac gaccagattt atcatttggt acttcaccca
    11461 agtactttgt attaaataaa gtcgctatat cattagcaat ttggtcaagt actctaacac
    11521 tttggttact tgaaaaatcg tcatttttat catctgtaaa tgatacaaaa gtatttatgt
    11581 cctctaacac atgaacttca tcaccaactt tatgaaatat aaatttacca ctctttagtg
    11641 cttcttcaag ttgtatttga gtgtaattta caccaacatc aaactcacca tcatactttt
    11701 tattagtatt agatttattt atatcgcatc ctgctatagc tccagtagcc caataaatca
    11761 agctagattc aactaaatct ttatctttaa tcttattttc tacagacact acaccttcat
    11821 aatctgcatc acttttctta tatagtactg tttgaaactt tgctcctacc ttatctctca
    11881 ttctctttgt aaattctaca aataaacttt taatctctgt tgttgtagcc aaacatccta
    11941 aagcattaaa tgaataactt tctattttat ctaagaaagc ttgatattct gctcctgtaa
    12001 ctgcttctcc attagttcca tttgtaaaag ttaatcctgc tgtagcttct aatgttgcat
    12061 ccttcttcca agtgatatag tcattgtctt gtaagtctgt aatgacttta gctacttgaa
    12121 tatctacctt cttattatct aaaagtgtta caacatcaaa cttagcatta tcatctatat
    12181 ttgttgtaac tattatcttt aagtcattac ctcttattcc actatactta gctatagcta
    12241 tagtacaact ggctttaacg cctttattta atttataaaa atatcccaac cttatatttt
    12301 tgaataaatc tctcaagcct ttcagcttct catgagtata atcatatcca aaatacttca
    12361 ctgaatactt ctcaaaatca tcacttgtga cttgaaatac ttcttcatct atgccccaat
    12421 ctaactctaa aggcattgca acaatacctc tatccgataa tgaactggtt gcccttttag
    12481 ctgagataaa atttatatag ctacctggta atactttatt ctgtgttaca aatgttcctc
    12541 ctcctaaagc catctaactc actcctttca taaaattatt tattattttc tctacctctg
    12601 agaaagaata taactcattt tcttttaaaa ttgcatttaa taagtctttt ctatttacat
    12661 actttttaga attaactatc tgctctttag taaacttata gtcatcttct ttattcaatg
    12721 ttttactcaa aattatcacc tctcttcaaa ccaccgaata actctactgt atccatctta
    12781 tctgtatcat tactttttat agtaaagtag ttataatcaa caaagaaatg aagaacatta
    12841 tctacaattt caaagttcat gtttgtacct ctgactaaat ctccatcaat ttctatatac
    12901 tctaattcct ccagtagcat ctcagctacc tcatttattt caaatgattt atcattactt
    12961 ttaggaaaat aatgtacatc aaaagaattt ttctttaatt ctctgccact tggatatgat
    13021 actttgcttg gatttaaagg aacaataaaa aaacaaggtt catttatacc ttgctccaca
    13081 tcttcgctat aaattgtata ttcatttcca aacgatttat ctaatttaat agatattcca
    13141 tctataatat tattaagcat caaacactcc tttaagcaat attaatagct ttttctctat
    13201 aatcttatca atctgacctt gtaactccat ttctgaaata gttaaaaaat gttgtccttt
    13261 aacccatccc ttaccattct tagtcctatg cccaaactca acatatgaag catattctgt
    13321 tggattgata acctctatga tgtaattatt accactttta tacactggaa gtgacctagc
    13381 ataagctatt ccactccaac cttctcttag aaatcctgta tcaactggtg ttcttctaat
    13441 tactttacca agcagtcttg ctgctaattc tcttgctgca tctttgcaaa acttatctaa
    13501 atcaatcttt gtaaatttct ccattcttct acaagctctt ttaaactctc taaaatcaac
    13561 actgccccat ctagccatta tgccttgtcc ttaagtaatt ctaagataat ttcttgatgg
    13621 tttggatata tagctgattc tccacttctt acatattctt tatcatttat aataagtttt
    13681 gaacctgctt taatgtttat gtttggagat ataaagagtt taatagtctg tacaagttta
    13741 gctacttttt catctgtagt tgatgtaata tttttatatg aaagcttaca tggttgattt
    13801 tctaatacaa tcacttcttt attgttagtt cgttttgtta cagggtcttt gattggctga
    13861 tactcaacta tagtacattt atctctatat aacatttcta ttgcttttct agtcttatcc
    13921 atcattattt ccacctaatt tttctgtatc tatttagttg tgctttataa tctttaagta
    13981 aagattcctt aaattcacta gctgaacttc tatatgaaat agatgtgtca ccttctgata
    14041 tagaagaaat agagcctagt gcaacatctt cgcttcctag actctcattt ttatacattt
    14101 ctatagccat tcttaaaata gtactattta attgttctgg tatagtaggt acattacaat
    14161 agtttttgac tatttcttct acatcttcta gtataaactc tagtattgta tctttagaac
    14221 tatcactctc atttaaccct aagagtcttt taatttttat tattttatat tccatatcta
    14281 tatcttatgt ctaaaagcta ccattctaat ttgtttaggt tcataaacag gtttccaatt
    14341 agttgcatct gatagttcag ctcttgaagg accttctgtt ttagccatat tttcatttgt
    14401 aaacttaact cctctagggt gcatgataaa agtctttcta tttataatat aatcaacacc
    14461 agaacctttt ttcttatctc tgtctatttc tgtagctaca aatcctactg gattaccatt
    14521 tcctaaagct attgcacctt caccaaataa atacgaagta aatactatat tagaaccgct
    14581 tccttcataa ggacaaccat catcaattat aaccctttta ttttgataaa aatatattgg
    14641 accactatta gaaggttgaa cagtttcaat taaattttgt tttcttagtt cagcttcaac
    14701 agcactgtgt actgcaactg ctgttaaagt ttcttttgca tcacctaact tttgttgtgc
    14761 atcaataaac gctgaaccac tccattttgc agcattacca gaacttgaag aaatatctag
    14821 tatatttgat gctaacctag tttctgctac tgtttctcca actgccttag tagttccaaa
    14881 tactccttta agaatagcaa ttagttcttt ttgcatatct ctagcccaga aaccagatac
    14941 taattcagca atagcacctg ctgggtcttt tcctgctaaa gcagcactca aatctgtagc
    15001 actccacatt tttgctcttc tcataattac tgcgacatct gcatttgaag ttattttatt
    15061 attttgcaaa tcaacatctt caattatttg ttctgattca cctgttaaat cctcaaagaa
    15121 tggcatattt acaagtggac ttgcttgaga agctaattta tcaaactcag agttattagt
    15181 tataattcca ctttgtaata atgcactttt ttccattgtt ctatttatta catatggatt
    15241 aaacaagctt ggaacaatta tatcacttag ctttgttcct ccttcattcc cagctaataa
    15301 ttgtatattc atttttagac ttttattttt attcatattt acctccttta tgttatttac
    15361 cagcagcagt cattaattct tttgctagag ctgggttatc attatatatt ttgctttgct
    15421 ctgttaaatt aaaacttttt tctaaccaag gattagaacc tttataatca tctccacctt
    15481 taggaatata atttggttta ccaccagtaa ataaatgtgg agagttttcc ttataaactt
    15541 ttattgtgtc ttctaaacca attacttttc cttcattatc aaaattaaac ttttcagttc
    15601 ctccttgttt ataaattata taatcagcat ctacaacacc taaatctttt aatttgcctt
    15661 tcaattcata ttcttttttt gagatttcca tatccttttt tagcttttct aaatcattat
    15721 tatgtttatc ttttagattc tctacatctt cttttgtcat acctccttca aactttttaa
    15781 tagtttcatt agctgtatta agctgtgttt caagatttgt ataatcttct tgagtaactg
    15841 tagtctcttt tattttcttt tctatagact tttgaagaga agctacatca attttgttat
    15901 cttctacttt tattccttct aataattctt ttaaccaatc catattttaa atatctcctt
    15961 tcatttttac ataataaaaa agccttttaa aagactttta agtaacttat ttactaccaa
    16021 taaaaatacc tgttagcgat aatacaaaac agattattct tcctactaaa tatcctgcta
    16081 tcatgcctgc taaattaaaa acaagcttca tacaagtaaa agtattagta tctatcatat
    16141 tagctacatc taatacattt gctataatta tacttaaacc tacaataaag cctaaagcta
    16201 tcccaacaat tataataaga actgatataa tatctctaac acttgattta tttggctttc
    16261 caatgtgtct ttttatatat cctcttgtgt cttttttaaa catattttca ccccccaaaa
    16321 tctactcaat actttctctt aggcattccc ctaactgttt tacaatctta tctatatcaa
    16381 ctttacaatc acatttattc attttttctg ctagtttatc aattttatca tctgcttttt
    16441 ctaaagtttc tataaaaaac tctaattcct ttgtggcatt tttaatattt tcttgaaagt
    16501 ttgttgaatc taaagtcatc tctgttgata atttattttt tcctctatat tctacttttt
    16561 gatatgtcat ttcaaatata tctgctttac atggatatat ttctcctttt acaccttgta
    16621 taatgtaatc ccctttatta gctctcatag taccttctaa tgtttctaag tcacaactta
    16681 catgtccatt aatgcatttt tcttttataa aattacaaac tctatcattc tcaataaacc
    16741 atttcggttc tacatcataa cctagtttaa aagcttctac ttcaactggt ttcttcacaa
    16801 atttagccat attacttatc cccctttaaa tttttaatta tatcttcatt gctaactagt
    16861 aaagaagata tgataaatat cacacctaaa ataaaattaa gtagtggaaa taaagccata
    16921 aaaataaaat tacactttct tctcactttt ttatttttaa gaatctcaat caattcctca
    16981 ttactatcaa tcttcatttt aaataaataa agccctgtac aaaacacaat tattgataaa
    17041 atatataatt taagcatttc aaatcaccct ctcaataaat ttttacataa taaaagcacc
    17101 tactaattta taacttagca agtgctttta catatttact atttgtatat ctttccataa
    17161 atcctttagt aatttaccat caatgttgta attatcaacc atatccttac catttttata
    17221 atactttgta tctccattag ggcaaagagt aataaacctt gtatcatcat ctccaataga
    17281 tatattatat ggtttattat ataagtcaaa ttctatatct aatcctaaat caatagaatc
    17341 aattaaatgt tgtaaattct taaatttatt atccatttta ctctcctttc aaaatatctt
    17401 tgtttgctat tttatgagct tttgtaagct ccatatcctt ctctcttttt accttatcat
    17461 ggttattttc atcagctaac caatcatgtt tatgaggtac aattttatgt tgctttgggt
    17521 ttctatggtc agttaagtct aaatctaatc taggttttcc tgtattacca tagtatcttc
    17581 tttgaattaa tttaccatct ttgtaattat caaatacact atttggtttt gattcaaatg
    17641 gcactgaatg aacacttcca ctagttaaat ttctctgatt cttaacttgc caatttacat
    17701 ccttataaag ctttttagct tcctcatacc ttatagtatc attatacttc atatgttgat
    17761 attcatcaaa tttagaaggt atttcatttc ctaatacctt tttatattct tcaaattgtt
    17821 ttctatcttt actctcattt agttgcattt ttctaagagt atcagctttt tcttttccaa
    17881 gtctaccctc tatatgtttc tcataccact cattatactt catattagat ggtacataat
    17941 atgtttttcc atcttctcct tttgctgctc tgtaaccttc ttcatcttca aaccaaggag
    18001 ctgttgttgt cctacaatga caatgaaatg gtggagctgt aactccaact tgataatcct
    18061 tcatatcaaa tacttttcca tctaactctc tacatatatt tgatgttttt aagtctagtg
    18121 tggcaataat ctcatacttc tctacatcta aatcattgaa acaatctttt cttgaagctg
    18181 atgcaaagaa agctgattca gtcattatca aattcttagc ttgtgattta gatacattaa
    18241 atctcttaga aaagtcattt actaggttct ttggattttc acctctaata attgattgag
    18301 ttagcttagt atgtaactca ttaattaaag caggtctatg tttgccccaa atcctttcac
    18361 taaaatttaa tccatcacta gtccatggtt tagagataac tttatttatt ctattagtat
    18421 caagattcat taaactccaa ccaacgttta ctccttgttg aacattaaaa gctgcatgat
    18481 aatatccact tgtataaata tgtctcatta atttatcaat accatcaagt tcatttccat
    18541 aaagcacttc cacttgttgc tgtatttgta actttaaagc ttcaagcctt gttatatgaa
    18601 ctcttgcact agcattttct aactctttca tccacttttg atttatagca ttttctttac
    18661 catatttaat atattcttct acactccatt taaactcttc tagttctctt gtatttagta
    18721 gtttcctagc ttctaataaa gatattcctt catttttggc aaatctgttg taccatgcta
    18781 atatatcttt ttctatacta tttatagcta gtttatattg cttttctaat tcaagataat
    18841 attttacact tttgttattt tgagcttctt ctaattgttc aaatctcttt ctccaataat
    18901 cattatgttt catctatgcc accgtcttga ttaggaatta aatcatcata ttcttgttgg
    18961 ctattttctt ttttaatctg tttttcttct tcctctgcat tatctacaag cggatgattt
    19021 tttaaattag tcttttctga tattattcca acactcttag agcatatctc agctagttct
    19081 aaatcatttt gaatcatatt cctagtccaa gtttgcaaaa cccttttagg agaatatcct
    19141 aaatgtctac atatcattct tactagttta gcaaacccta atctaaactc tgtttccata
    19201 agtcctgctt ttaattctaa caaagtgtac aagaatttga gtgctacacc acttgtattg
    19261 gcaaagtttt cgggtttagg gtcaacacct tgaccttgga catagatttg cttttctgtt
    19321 gttttaagaa gtgaatctcg agcttcaatt ggaatattta ttgtaattgt acttaaccca
    19381 ctcttatcat ctgcaccact actttgtaaa tcaatagttt tatattcttt aagtcctttt
    19441 aagaactctg ttaagtctgc acctccatag ttcgtaagaa cgaaaataac ttcttgtata
    19501 tcttcaatat cattcacaaa accgctataa accttgtcat atacatcaat gaggtgttta
    19561 acattatcta aatctcttac ctctaaatca ttgtttagaa attcgataaa tggaacttct
    19621 ccaaaattgt gtttatatac attagtttga gtttcaagtt tattatctaa atttttctct
    19681 ataaatttat taagtatctc caatccattg atattactat ttccatcttt attttgataa
    19741 gtataacaac attcatctgt ccaatactca taaattacat attcttttcc tttatcatca
    19801 gtctttttat attctctaag tactgcaaga agctttctgt ttaaatctga tgaatatact
    19861 gctcttattt ggcgagggtc tatattagca tattgaaagt tattattatc atccacccaa
    19921 atatgtaacc atgctttaga atatatacta gcatttttgc caagtgtttt agcttctttt
    19981 gggtatctat cgcctaaaat atcagttatc tttgaattta ctctatcatc tccaacatca
    20041 aatgtaggtg gataagtaaa cagatatgat attttttggt taactaaaaa accaaaccaa
    20101 ttaaatggta ttctattatc tgcatttctt agtggattat tagttgtatt tacttttcca
    20161 atattattag ggcttctatc ccttataatg tcattttcat ttttatagta tttttcagct
    20221 tcatcagctt ttttaacaaa gttactatgt ttactattag tttgttcaat taactttttt
    20281 attacatcta attccaattt atcaccttct tccatttggt gtaagtactt taattcctgt
    20341 tcctaaggaa tctgtataga tggcatatct aagtgcgtct aatacatcat cccacttttt
    20401 aattggctca cctgtatttt tattccaagc atacataaaa atttctttcc tgaataaatt
    20461 aacattctca tgaactacaa ataaagtatt agttttaatt cttttagcta cagcttcaac
    20521 gccagacaat acagctttat tagcattaaa agcttttata ccatttcttt taaatgctgc
    20581 caaatgttcg ggtctagcac tatcacaata aaactttata tttccatact tttcttttat
    20641 acttttagcc ttatctatcc agtaatctat ttccttatgt tgagctgaat gttcttctaa
    20701 taaatataag ttacctttat catcttctcc aattacaaca atagctccaa aatgttcata
    20761 tccccaatca acgcctgcaa agtatcttac aaaattaata tcatttaatt tatccttaga
    20821 aatataatga atatccttat taaaatcttg ataaactaat ccatctgctg acacccataa
    20881 accatttata tctctgtcat aaaaaacgcc actaggagta gacttcttaa tatttgctct
    20941 gtatctttca cttaaaaata tattatcatc taatttgtaa tgaaaagatt gaataacctt
    21001 gccatctgtt ttatctacaa agttagtttt taaccaatgc tctggttggt ctgggttagt
    21061 atctacaagt attctagcac cttctccact acatcttgat ttgatttcat taaatacttc
    21121 ttcatttgca actgtacctt catttatgta tgccccaaac gaagtcatac ctcttattct
    21181 gcctaaatca tttgtttttg agtgtccaaa gcaacatact tgaactccaa acaatacaaa
    21241 tctattatgt ttgtcaaact taaactcaat gtcatactta tttgtaagct cacttagtac
    21301 atttctttgt aaagctccta aatctgctcc tgctaagatg tattgaggaa gcttaatacc
    21361 tagctcatta gctatctttc taacccttct aagttcaagt aagaataaat cattgtcaat
    21421 tattgttttt cctgttcttt tagcaccata attaattagc atgaagtagt cattattcaa
    21481 agcaaagtta agaacttcaa gttgtttact atgatataat tcatcaatca ttttttatca
    21541 cactttcgag tttatcaaaa tatctatcaa gtttatcttc tttactttct tgattattta
    21601 tctttgattt taatacttct actcttgctc tttgctcctc tgtagctaaa ttccaatcct
    21661 tatgaatcat ttcatcatat tgtttaatta aactccttaa ctcactcatt gccctactct
    21721 gtgcattaag aaaagatgct tgcctatccc atgcaaattg aaattcatac tctatctttt
    21781 caccattttc tgtgctttca tatttcttta attctttaac catttcttcc ttgtctttaa
    21841 catacattat cttttgtgct cttattattg ctgcatattg tattgttatc tgttcccaaa
    21901 gaatatcaaa tttatcttta tcctttatct cattaattaa gtcttgggtt tcttcaggta
    21961 agtattttga gaagaaacca aatttttcag catttttatt acctggtgga ccagtagcgt
    22021 ttttattacc tatgggtgca cctctctttt tggttgcaac tttttttgat tggttgcaac
    22081 ctttttgttt ccagtatcta gttgcccatg attttacagt cgataaactt acattatact
    22141 tttcagctat ttccttgtac ttaagccctt ttaagtaatc ttcatgagct aaatctgcct
    22201 tttcattcat accaccacct cgtttgtttg tcgttttggg aacaaaaaaa gaactctggt
    22261 tagagttctt aatcttttag aatttaccat taaacgcttc atttgcattt ttataaaata
    22321 acttatatat ttcagcaatt ttttctggag ttaaagaagc attttccata tatgctattg
    22381 taagctctgt agctacatct gcaattgttc tttgacaaga aacacatatt tttccatcag
    22441 acataatagt cacctcctta taaagtatat aattcaactt caaaggtcaa tatccttcaa
    22501 aaactattcg acaactacaa aataattcta aataatacac ttatttaatt tattatataa
    22561 aaaaagacca tccatcaaga cagtcattta aatcatttct attaactctt taatcttttt
    22621 atatacctct ttataattca tatctttatc tattagctta ggtaatttca tagatataat
    22681 tctttcgagt gcttgtatat caaacaattc actttgattt aattcatccc ttttcacacc
    22741 ttttggtata cctaattttt ttcttacaag ttcagtaaaa tgtttataat acatctgagg
    22801 tttattactt ccttgactag tagcataata aacaaactct tgtatttcat ctgtaaaatc
    22861 ttttcttacc tttttaccct ctgctcttat atccagccat tcttggtctt tttctgtagc
    22921 aatgtaataa ccatgtattc taatttgttt aagtgtttgt gtaacccatt ttgtaaataa
    22981 ctttgcttct ggtttattac ttctaaatga catattgtac acagcttctt ctgtaacaaa
    23041 agtagtaccg aagttaggta atttatcttt aaagtttcta gtgtaggaat ctccgacagt
    23101 agactcatta aatttctttt tatattctct atctatattt cttaatgtat cacgaatatt
    23161 tactatgcct agttcctctc ctacgtcatt tgcattaaac caaacttctt ctccattttt
    23221 ggaccacatt acttttacat ttttatcttg taaaattttc aacatactac tacctcctaa
    23281 attagttatt aataaagggt gctcaacttg aacaccctta ttttctgtta tcagagttac
    23341 tgatatctaa aacttattaa atttttgtat aaaaaagacc tagaaattaa tctaagcctt
    23401 tttaatgggg gatacatatt attaagggag caagttctag gaatcgaacc tagattaaac
    23461 cactacctgc atggtgagtg aggttaccaa gccccacccg attttttaga tttctgaatt
    23521 aagatacaaa actgtatgag attttaatct taattcaaat acttaatata gtgtatcaat
    23581 agattttgaa catagttaga attgaactaa cagcgtcctc acgccctgcc tagtctgttc
    23641 atattgctag attagccctt taaactaacc tagcaattat ttagttttga gagggaaatc
    23701 tttcatttcc acaatattat tatctcatgg ttttgccaat aaaaagtctc acgatagtct
    23761 ccaaaaagtc tcaaaatagt ctcattttta agctttccat gaaaaaatag gcaactcaaa
    23821 ctcttttatt tttggatata acatatctat aatcttacaa actatcctct ttcttattct
    23881 aaagcaatga cttctatcta tgtgcatagc attagacata taatccatgt ttatcttttc
    23941 attgttcatg tacatttcat tgaagaactc tgtttcaaag ctatttagac ttgttaatgc
    24001 acattctata gtttcttttt caatttctaa tgttttctta tcctctttta atctatttaa
    24061 atcttcttct ctcttaataa cttcattttc aacacttgaa cttatattat aagtaggtcc
    24121 tgttttttct tcataacttt gagccttaca tccacaaaat tcattttcta attttttaat
    24181 atatatatct tttattctta tttgactttc tagtttttta tagttatata atctaccttc
    24241 aacctcttga aatagtgtct ttttattcat acttccacac tcccatcaat tttttatgtt
    24301 tttatgttat aataatattt gtatataaaa gttttatatt tttgacaagt ggagtgtgaa
    24361 agcactcctt ttttcttttt attaacagaa attatctttt tcaaagaaac taatttgatt
    24421 tgtttttctc tcagatttta taattctgat tgattcatct attaagttta atgaattaag
    24481 taatacatct ttcgggatat cctcccattt gtcagcacct aatgccaata gagttctttt
    24541 cttaactaat tcaaattctt cattaacttt tgatatacct agtctttctt ttatataaga
    24601 agatatatca tatttagttt tagaggttgg tctataatat tctgaacact cttttttaag
    24661 ttgctctatt tggatattat gtttaacttc catcttaagt aatgattcat tcacaatagt
    24721 attaatttga ctaagctgtg aatttgataa ggttctattt agtaactttt ctaatcttat
    24781 aaaatatctt cttatttctc ttcctttatt attgttttgt accatagcaa gttcttttgc
    24841 tacatcaagc ttcaatacat attcttttga aggtctccct ccagttgagt ttttcatatt
    24901 tttgtgaaaa actgaataat cctcattttc cttaaatcca tattgtttaa ttctatcttc
    24961 aatccagtct ataaactgtc tcttaacttc taagttatta tgtagttctc ttgcaaaaac
    25021 tattttctct cctgtatcag tttcataaac tgtaactaaa tcatctgcta caactcttaa
    25081 attttcattt gtcataatct cattcatatt tatagtcctc cttaaataat atcttctaat
    25141 ataacctcaa cccttggctt atcactatag tatttactag ctacaacctc aacaatctgt
    25201 gtatcatctt tataagctat ctcatttagt gaatcagcta taatcttaac cacattatca
    25261 atatcgggtt tcttgctagg tcttaacaca ttatttcttt tctgctcttt aactttttta
    25321 ctgttacttt tagctataga atagtaacat cttaaagtca tttttatata accttcaaag
    25381 taatgtttaa ctttagattg atatagccat tttattaggt cctcgtaatt tttagtttta
    25441 ttaggtgtat aggtcctttt agttatagag ttcattctag gtctttcttt gccaactggc
    25501 tctccatcta ttacaagaaa aactttcatt ttttcacctt cttagccttc tttctacatt
    25561 ccttacaaca ataaacatcc ttagattttt cctcaagata aaataatttg ccacaccaac
    25621 tgcatcttct tcgtttcata aactcacttc ctagtcacaa aacatttatt ttattcctaa
    25681 aacaacatac ccatcttgca gataattgct attatcaagt aaataagtaa tttctttgcg
    25741 tatctcaaga cctgtgtatt tttctccatc aaattcctgc aacaccaaca agtcgccttt
    25801 tttaaaacct ctatcatttt tcctaacttc aaaatttttg ttcccattta caacttcttt
    25861 aaaatattga ggtaatattt ttaattcatg tatcataagc tcacaccctt taaaagtttt
    25921 agtctatttc ccctttttca acttgttcat cttctatttg cgatatcact cgttcgtatg
    25981 ctatacgacc ttgccattct tttattgcag cattttcaat atcttgtaca aatatagcta
    26041 acttgccatt tacattcgct atttttaacc atataagttc atgtgtgtta ttcttttcta
    26101 cccatatggt aatttcctta ttttcttcca aacatatatt atttagatat atctcattat
    26161 ttatcttata catttgagta gtaatcataa tctcgctcct tttaaaagtt aattttctca
    26221 aaaatttttt tatatttttt aataaaatat agcaatacca atgactctat tttatttttt
    26281 atggtcctac aattttttaa aacttttaca gttttctcca ttatttttaa actttttttc
    26341 atatcctctg ctacaatact cttctctaca tatacctcct tcataacttc catctgagta
    26401 aaaataaact tctccatctt cctcataagc tctatatttg caatcttcac atttattcat
    26461 tttattacct cctatttttt aaaaactccc ttttataagt caaagtaagt ctatagactt
    26521 ctagtttcat tcacaaactt accttgacca attattttat ttattttttc ttctttcata
    26581 gaaatcttta ttaatccatg aagttaaatt ttcaaaataa aatgcttctc cacaaatcgg
    26641 acatactggt aacatttttt tacccctgta tttactttca agcctcttaa aaactatcaa
    26701 gtgaggttta taactagcta tttcttttct ctgttttaat aatgtgttta cttgcccatt
    26761 tactctttca aagttcattg ctagttcata tagggcatca tatggttcta ctctaaatcc
    26821 acattctgaa caatgaacag atttattgac tgtatctact ataaattctc tattcttaca
    26881 cttacatttc ttatcattat ttctattaat tcttaatata tcaatttcga taacattatc
    26941 tggtaaatcc atttctaaac cactccttta ctaaagaaac ttatctgtct ttcattttct
    27001 atatcttcat tactaaatcg cttttctagg tcattcactg tgaccccatt agctctaaaa
    27061 ggtacagggc tatctctatc aaacttaagc atcttttgcc acaaaatggg atagtatttt
    27121 cttagtgttt ttaggctctc taagctttgt ttagggcaca aatagcaacc agttctcttg
    27181 aatctgtgat gtatctcata gtaaaagtct ttttcctcta gatacttcaa acaatcactt
    27241 tcagtcattt tttcttcaaa caatggtgct atacagtttt tttctagtct tttatatctg
    27301 ctaggttcat cgtacgcaat tccaatatac ctcttatgct ctcctagttt attaaaatat
    27361 ttattagcag gagcaagctt taatctgcta ttacaccatg cccctagagt atacggaaaa
    27421 ccccatattt gccctttgcg tttgcctttt tcatttacag tataaaagta ttcctcaaaa
    27481 gttttttctg ctttaattct tgttatttta aagtttattc ttttctcaaa atcatctatt
    27541 atatcataaa tttctttaaa ttctagtcct gtatctatga aaactatgtc atctaattta
    27601 agaccttttt ctaatattaa aagaagcatt gcagccgaat cttttcctcc actaaaactt
    27661 gcaatatact tcataaaatc actccttgca ttttctaaat gattcaacta acatttcagt
    27721 ctctccacaa gtatcttctg taaaatctat ttgtctccca ttaaattctc ttatataatc
    27781 tgctatatca tatactcttt gacatttatt ctctatacaa tggcaaatgt catttatact
    27841 tatttcttct ggtatttcaa ctataatttc atgttctaaa gttacttttt cctcaaactt
    27901 aattttatat tttttcattt taaatactcc tttatttcgt ttttgagagt tacaaaacac
    27961 ttcaacaata atttatacta aaagacattt tgcaactctc taaactgttt taattagata
    28021 ttttctgtat ataaaaatcc ttttaatctt tctatttctt tgcttatttc atcattccag
    28081 atgtttatag ctgtatctct atttaaagaa gcaacataac tagcactttt tacatcaagt
    28141 acatttttat taataactct taaatattga ctattactcc ccactttgaa tctacctttt
    28201 gttccttcta gtatttcaac ttcttctact ccttgtgaac tgcatatata aagtttatcc
    28261 attattcttc tacctccata taaagccatt ctttgaaccc ttcgggactt tcataacata
    28321 tataaccaca aggttctccc tcatatatac aatactcaca aatacaatct gtacttccaa
    28381 aaaaattagc cattccatcc tcatccaaac tttttatcat ttcaaaatta gtcattttta
    28441 gcaccctctt catttctcaa taattcatga gctttttctt catcataact gcaaatattt
    28501 ctaccatcac aataatgaca aagtaatcta gtatttttag taatgatagg aaacattttt
    28561 ttgcaaatct ccaatctatc atccattctt atacacctct tttgttatca caattttcac
    28621 actctttaag attcaatcta tactcataaa ctctaccagc tataaaacta attcctatca
    28681 gtagcacact agccaagatg ttcattttcc aacatctcct tacgttctaa gaactctttt
    28741 ttaatttctt ccaaattctc gcattcatta ccaactacta catatttttt gctattcatt
    28801 atagttgctt tacttgtaat ttcctcccat ttaccttcaa attgctttaa ataatgccat
    28861 tcaacatata attcaagagc ataattttct tgtcttacaa ttccatattc atatttattt
    28921 ttagtattat agggttcttt caatatatct ccctcataaa tttcttcatt atttccagac
    28981 caacatttag agaatactcc aacattagat actttttgcc attcgcaatt ctcttttagc
    29041 ataaataaac aatctactgc atcactccac tttattgttt cagaataaat ccacttctca
    29101 ttctcaaaat cataacctct gtacttaatt aaactcactt ttaatcatct cctcatattc
    29161 ttctctagcc ttatctatag caataaatat gtcctctcca ttgtcataca actcttttgc
    29221 tcttttaatt gtgtattcag tccttgaaac ttccattatt cctccttaat atattcagct
    29281 ttccagccac ttcttgtttt agttttcttt ttaattgttt ggtaaactgc ctgactctgt
    29341 agtcttaaaa aacacgctgc actatctata gaatcaaata ttttttcttc accagttttg
    29401 gcattaatca actttacctt tgaacctttc tttttctttt ttctattttt atcaacatta
    29461 aactctatta gcattttttc agaagttgga aatacaagct ctccattttt tcttactcca
    29521 tgaacacaac aatatagtgc taagtagttt ctaagagtca aatcatcttc aaatatgtta
    29581 tctgcaacag aaccattaaa atatttttca acctttaaca tttcagctcc ctccttattc
    29641 aactggcatt tcaaacactt tttctttgtt gcatctaacc ccgtctttat ttataatgtc
    29701 aaacttagtt cctgcaataa cagcattctg tatcatgttt aatacttcaa tagctctttc
    29761 ttctgattta tacctaccta tttctctagc atttgattcc tcttcaaaca ctgcacatac
    29821 ttgtttatta cttatttcaa ctctattaac tctcattaaa tctaatctat cttgacttct
    29881 aattattatc atccctaatc tccccctcat cacatcttct tatatcattt aacttactta
    29941 aatcttcata tatattccca tcaatcttaa ctattgcaag ttcagataat ctaaagcaat
    30001 ctcctgacat ttcattcact gcaataaaac aaccatcttt aaattttacc tcccctgcaa
    30061 actctgtaaa aaatatactt ctacataaaa caatatcgcc ttcatagatt tcttttccat
    30121 cacaatcctt taaacctgtg tatatcataa cctcaaaatt ttcattgttt tttggcaaat
    30181 aaacaccact ataaacctct ctaagcaaat ttttagaata gcacaccatt tcatcataac
    30241 tatacatttc tttaccattt ttattccatt ctctaaattt taactccatc tttcatccct
    30301 ccaatatttt tcaactccta ggaagtaata ttgtataact accccctaga ctatttaact
    30361 taattaaaaa ggtatatcgt catcatctat tgcttgaaaa ccttgtgggt ctaatcctgg
    30421 tagtacatat tcttgtttag cattattatc atttttacta gaaagtagtt ctaaggcatt
    30481 tacattaacc ttagtaatag atttccagca accattttca tctttgtaat tatatatatt
    30541 taactctcca atagcataga caggcttagc tttaacaaga tattgcacta gattctctac
    30601 atgttttcct aattgttcac attgaataaa atcagttatt ttatttccat ttttatcttt
    30661 aaaccttcta tctactgcca ttgaaaaggt tatttttgga gtacccgaat ttggaaggta
    30721 cttcaattct gcatctgcaa ctaatcttcc aactaaagtt attgtattca tttaactagc
    30781 ccccttctat ttttcttcct gctcttctgt atactcaaca aagtaagtat aagttgtctt
    30841 gctattttgc ttctctctag caatctttac tgtatatcca gctttcccaa gtaatcttaa
    30901 taattccaat ctatcttgtt cgtttaaaga accacttctt tgtgcatata ttctcgccat
    30961 tttatacctc ccctttctag gaagtaatat attgatattt acttcctaga agtataattt
    31021 tatttaaatt taaccttttg acttttctta attatgtcat ctagttcgtc aggtgaatat
    31081 tgagtaaagg tttcattgaa gttatgaaac ttatttttac tcacattagg agtattcaca
    31141 tttttatgat tagactgctt cttctcctgt ttattctttt tcttcctctc aaactcattt
    31201 tgatactctg taagttctaa attagttttt acacctgctt ctatccaatt gtttaatatt
    31261 gtctttacat acttataatt cttaactcca tttgatattg cttcatcaat agctcttata
    31321 attacatcag cttccattcc atcatctaag taactcatta actctataaa gttattagga
    31381 gtaatcacac ctatatattt ttcaaagtat ttttttatat aggtggtttt atcttgttga
    31441 ggttgttcat taataacaat agtagtaata tcattattta ctttaaagtc attacttact
    31501 acttccgtgt tttccggttt ccgagaaacc cggtttccgg gaaatccggt ttccgggaaa
    31561 tcagtttttc gggattttag cttctgagga ttttcaagtg gtatctcata tacttgataa
    31621 tcatatcctc caagcatctt attagtatta gaatctcgac aaggtgttct tgttatatat
    31681 ccattttcta tgagctccct taaaatattt gctgtagcat ccctcccatt tttacttctt
    31741 ttatataaat cattaacata gattttccaa tggtcgggct tactaatcag atatgaatgt
    31801 aaaccttttg cttgccagct taattttaca tcttccaaac aagttttatt taaaaccaca
    31861 tatggattat ctttgtcttt gcttactctt ataatcccca caatatcacc tgcccttatt
    31921 gcttattttc ataagcatta caaatagtgt catactcttg ttttgttaaa tcttttatct
    31981 cttttccaaa tcttttaaat actttttctt tcaaactttc tttatcaaca tttgcattac
    32041 ttgctattgc atataaccta cttaattgtt tatctgttaa aattctattg ttagaattac
    32101 ttttagcttc atttttgcca ctggttgcgt caaaagtgtc actttcagtt atattaagta
    32161 actgaatata taaatatcta gtttgatagg tttctatacc tcctagtgct tgtaattcat
    32221 tagaaccttt aagttgcaag tctctcattg gagaagtgaa tacaatctgc tctgatgggt
    32281 tttctccatt aattaatgtt agagttgcat attcatttgt aaaggtcact atagggcata
    32341 gcttagcttc ttcaagtaat ccagttgctt gtggtagaaa gtctgctaac tcaaaatact
    32401 tgaagttagc gaacttattt tctccacttt tctttaaatt caacttacta aatttaactc
    32461 ttacattcat caatttaatg taaatattat taatttccat ggtcctcacc tactcttttt
    32521 tagcttttgg aattgttagt gtagttccat attcaatcct gcaaccttcg acctcatgac
    32581 cttttttaat aaagtctttt atactatttt tatctacttt tacaacttgc tctactgttt
    32641 tatatatagc aggtatcttt tcttcatctt ctatgactaa gctacctgct gactttctta
    32701 tacttatatt tcctaaaaat gtttctactt ttttagtacc aagtagttcc atacattcct
    32761 ttatattgct ttttaatctg tcaagagcat tcttcttaat cttctttaac tcttgcattc
    32821 tcttaatctc tgaatctata gagtttatat cactgtcaat gtttaatatt actgaaacta
    32881 tcctagtgtt tttattttgt atctcttgtt ttattatttc ttttatttcc tctagttttt
    32941 cagtttcatt tcctgttgtt tctgttaaac cttcttctat ttctaataaa tctgtagtta
    33001 attcatataa agtactcata atttccctcc gtttgtgcta taattagctt aattaaattt
    33061 tgatatattt atttgaattg agccacggca atggctcttt tcttatatct gaacatctat
    33121 aggtctatct ctttcaagtt cttctaaaat taattgaaat atcttgcaat cctcattttc
    33181 ttcatattct tttatttcaa tttgtgtatc tataatttct agtaatgact cagcaaatat
    33241 ttttaatctt tcgtttacac ttttttctct taaagcatta ctcaattcaa tttcttctga
    33301 tatatttctt tcttcttttt ttctaagttt tgtataaagt tgctcgtttt tatttatttc
    33361 taaattagct ctatttagtt gttgttctac tgcatttctc actataatta aacttttcat
    33421 gattaatccc ccttaattta gcatttcaat acaataatca tagcttattg catcatctat
    33481 agttatagac gattgtacta aatcatctaa ttctttatca aaataaacta ctgttaattc
    33541 aaattcttgt gattgagtta ttatacaatc gctttcaaaa ccaaatcttg cacatgttac
    33601 tctgattgct ttacctgctt taagaatttt tgtaggaaat tttactttaa gtattttcat
    33661 atcactatcc ccttattgta ttttttaaga ccttcaaaac ttgctttctt attgtattgc
    33721 ttacagaatt gtacataagc tatcagtact cttacattca actaaatcac cccctttctt
    33781 tttttcatta catctttatc tttcattgca tttttcataa taaattcttc aaatgatatt
    33841 ctatcaatca tgtactttct tcctattttt aaagcaataa aatcttttgt tatcatggct
    33901 tctcttgcca tgtttctagc tgttacatca gatattttca agtactcaca aaattcttct
    33961 atagtcatta actccattct tttaattcct tctctatcta aaaacatctt gatgatgtct
    34021 gttgtatcat ctcttcgcat taactcttgt actaagtctt ttgtgtctat gaattgtaat
    34081 gctacactca ttttcaaacc tccttctcat attaattgaa tatcctgtat ttagttttca
    34141 aggtactgtc atgatttaac ctaatttttg cttaaatcac ttgatattcc atattttaaa
    34201 gccatatctt ttacaatagc cacatacccc tctattagct tcttatcatc ttgtattaca
    34261 tctaaattgt taactttctc tcttttagat tcagatacac cttcttctgc catttttctt
    34321 cttttattga ttaatcttct atgtaggtca actccaaatc tcttatttaa taattcataa
    34381 ctttctgttc taagcatatt tatatgttca aaaccacctt gtttttttgc tattcttgca
    34441 attagttgat gtgtatttgt tctccaacta tttgagtcta atgaaactac atcttttatt
    34501 gtttcaacct ctgtctttgc ttctaaagca atgctatttg cttgattaac ttgaagtctt
    34561 aaatctttca tttcttttaa actttctatt aatacatctt ctatacaagt tggcttatgt
    34621 tgcttaactt tgaaatatgt ttcttctaag ttatcaaact gctcccaagc tttgtcagta
    34681 tccaatattt tgcagtgtct atttgctcct ctttcagtcc aaagatacat ttttgaagta
    34741 aattttaggt tttcatattc tgtatgaata cctttaaaat tttttaaatc atcaccttgc
    34801 aataaaaaat aatgtttacc ttcaataaat ctatctttgt tattgttaaa attgttgctt
    34861 atatttcttg catctgtttc atatacatct gctagttgct gtgtagttaa aactctttcg
    34921 ttatttcttt ctattacttg taagttattc atatttttca gctccttttt ctttaaatgt
    34981 acttttagta cagttgtttt tcaaaaaaat atactctatt gaagtattga aataagctgc
    35041 tatttttaca gctgtattta gagacggtac tctttcgcca ttctctaaga aagctatata
    35101 cctacttgta agacctaatt cagctcctaa ctgatttcta cttagtcctt tttgtattct
    35161 aaaatctttt agtttattca ttttttcacc ttcttttact ttatcttatg tactaatagt
    35221 acagtactat tagtacattg tcaatagctt ttttaaaaaa aatgagctat aattgataat
    35281 taagaactaa tagttcaata taaattaaaa aggtggctaa ataacatgat tggatataga
    35341 ataaaggaat taagaaaaga aaaagatatt actcaaaaag aacttgcaac ttttttaggt
    35401 cttactccta aaatgatttc tttttatgag aaggaagaaa gatttcctcc acatgacata
    35461 attttaaaat tatcagattt ttttaatgta tctacagatt atttgcttgg aaaggtcaat
    35521 gtaaaaaata tagacaatct tagtgagtta gagctaattg aaaatctaaa tttctctgat
    35581 gatataaaag aagctttaaa actaattagc gaattaagtc cttctagtca agaaaaaatg
    35641 ttcaaaatag caaaagtatt tcttgaagaa gaacttaatg agaaaaaata agaaagagaa
    35701 gaaaactatt ctctttcttt tttattttct tgtttataat attcttttaa taattctatg
    35761 tacttttcta acttctcact attattttct cttaatttgt tcaatgtatc ccctgtgtca
    35821 attagtagtt tttttgattt ttccacacgc cttcccccta ctatcagaac ttacgttctt
    35881 atttttagtc aaaattccct aatgaatttt aataaaatat aactatgtat tttcatttct
    35941 aaaaatattt ttagattatt caaaattttc ttggataatt atcttactta cataataata
    36001 cttttgttaa atatatgcaa taaaaaaagg ggaattgtaa caaaaaaatc gaattttgta
    36061 gagtcttggt tattttttcc attcctgtgt attattaatc gtctttttaa ttataatttt
    36121 ttgtattttc ttgtttatat aatatttttt attatttaat tttaaaatct tcgttttact
    36181 ttttatacca tataaatata gtattctttt caaaactatt taattctatg ttttttccta
    36241 atatacatag catttataca gaaagtttta tatacttaaa ttgttattgt cacaaagtag
    36301 aatacactag aaattaatta aatacaaaag gagtaatttt ttatgagcat tttgaatcta
    36361 tttagaaaaa ataaaaacaa aaataatgtt gattgcaatg aaaaatcgaa cgaatcaaaa
    36421 caaagctata atgatagtac tacactttca ctagaatgta attctaattc acctcataac
    36481 atagaagtaa aaaatgaaat taataaagtg ctgaatgtaa gttgtaatga tacaacaaat
    36541 ttatatgaat tagaaaaaca agcaagagaa aaatcagatg ctttatatga tatacatact
    36601 aatagtatta ataagtttaa cccacaaaac aatatattag aaacagatac taaaccactt
    36661 acatcaatag aaaaatcttt cctaaaatac ataattggag aaaatatata cgagccttat
    36721 atagcaacct attggacata tgaatataat attaactatt catatttaat ttctaagttt
    36781 ttcaacatgg attatcttaa gatttcaaat tacattgaag accttactaa gttaacagtt
    36841 tcggaattga aagaaatatt aaaatctaat aatattaaat caactggtaa aaaggctgaa
    36901 ttaatagaaa ggatagaaaa agaaatctca tgcaaagatt tatctaattt ttttaatagc
    36961 tccaacaaat attatgcttt aacagataaa ggaaaagaac tgttgaaaga tgtgcgtaaa
    37021 tctgttacta aaaatactga cctagaagac cagtgtttag aattaattta tattgataaa
    37081 tatgaggaag catatgactt aatttgtaaa tatgaatcct ctaaaaatat ccaaagagga
    37141 attaatataa actgggaaaa tcataaaatt acacctatga aaattgagtc ttacaaagcc
    37201 ataaaggaat tagatataaa tttaaaagac acacttttag ataacataat taaatcatcc
    37261 tatatacttt gtgatatgtt aggaaataac tccaaaactt ctatattagt aaaacgtttg
    37321 gtaggagaaa aaattgataa tatcgaaatt aataatgcta taaatagtat aaatgatgta
    37381 atatacaatc actctactat aacatataac gcaaatgata gtattaataa taaaaattta
    37441 cattatatgg atattgttaa aaataacaaa tcaataccat tcgaagttaa taataaaaat
    37501 tacaacttca aaattaatga aaatgaaatc tactttttta atatacttaa aattaaaatg
    37561 tacgaaaatg aattaaaaaa taattttgtc tttgatagaa tgtcagatgg agcatttaat
    37621 gtatttgata cttcagataa cttcattggt aaagttaaat tgcaaggtag aaaaaaatgg
    37681 ataatgtaca cgaaaaatga atttggttca gaacatattt atggcgaatt aattcactta
    37741 atcgatggta ttgatgcttg gattaaatat tcaaaggagt atctaaaata ataaaagagc
    37801 tacttaaaat gattaaaact aattttaagt agcttttcct tatgttcaaa atactgataa
    37861 catactttta taagaatgtt atattatttt tataatttct gttaacatac ctttataatc
    37921 caactaaatt cttttaaata actaccattt tgttttattt ttaccttttt ttgcaaatat
    37981 atggaaattg cacataaaat tttatatact taaattatta ttatcataaa gtagaacaaa
    38041 aaataaaaaa ggagttttga tatgaacaat gaaaatgaac agttaaaaag tgatttactc
    38101 atcaacgatg aaaaaatcaa aaatcttaat ttcaagatac cctggtatta ttctttatgg
    38161 actatatcca tactaattct ttctacgttt tcaacatact ctatatcatt tatagttgca
    38221 ataatattct tatttaaaag aaataagata atgaaaaaac ataaagatag cattagtata
    38281 ctattatctg atgtcgaaaa aatcaataat aaatatattt tattaaatga tgaaattaaa
    38341 atgaaagaaa aacactttga agatttatgt gaatcaaatg aaaataagtt aaaagaatta
    38401 tccaatctat tagacaaaaa gaaagttgaa attgataaat ttgattcaga aaaccaagat
    38461 aaatttaaac ttatcgaaga actaaaaata gaaaaagaaa gacttgataa tcttataaaa
    38521 gataaaaata tacttaaaga taatattaat actttaaatt ctcatttaga agaattaaaa
    38581 aatgaaaggg aagagcttag agatataaac acaactttaa aaaataaaaa agaagagcta
    38641 aaaagattat cagaagaatt aatacagact gaagatgagg ttctgcttca atcatttgga
    38701 ttatataatc caaaatatga ttttgaaaac tctgatgaat atatggaaaa attaaaagaa
    38761 ataagagaaa tgcaaaaact attaataaga aataaaacag gtgtaaaata ttctgattct
    38821 tggactgttg atggaagtgt tcaaaaaggt agaactatga ctaatcaaaa tattaaaaca
    38881 gctcttaaac tcttcaatag tgagtgcgac attgctatgt caaaagttag ttttaaaaat
    38941 attgattcta tagaaaaaag aatacgaaaa gcatttactg atacaaataa attaaatact
    39001 tcaaataagg tttctataaa agaaaactat cttaatttga aaatagatga gctttattta
    39061 tattatgaat accttcaaat gaaagaagag gaaaaagaag aacaaagagc tttgagagaa
    39121 caaatgaaag aagaagctct tgttcaaaaa gaaattgaaa atcagaaaag aaaactaaaa
    39181 aaagaagaat tacaattcaa gaatgaatta cttagactaa aatcaactat cccagaagat
    39241 gaaaatgata aattagaatg ggaacaaaaa attaattcta tagaagaaaa actagctttg
    39301 ctatcaaaag atttagatga tgtattaaat agagaacaaa atacaagagc aggacatgta
    39361 tacataatct ccaatattgg tagttttgga gaaaatatat ataaaatagg agtaactaga
    39421 agattagacc caactgagag aataaatgaa ttaagtagtg catcagttcc ttttaaatat
    39481 gatatacatg cgactatatt tagtgaagat gcacctaaat tagaatcagc tttacataaa
    39541 gcttttgata ataagagagt taacaaggta aataatagaa aggaattctt caaagttacg
    39601 cttgatgaaa taagaacaga agttgagaaa aatttcgata agactgtgga atatacaaaa
    39661 ttggcagaag cacaagaata tagacaaaca ttaaaaatac aagaattaaa taataaatta
    39721 gcttaaatat attcaaacaa ttatacttta ataaaatatt atttttgaat atctatacca
    39781 atttgtgata taataatagc aaggagataa aatttacttt ttaaaaggtg aatttctcta
    39841 attgattaga atttaaaagt tatttcttta aatcaccctt attggcgttt gggtgatttt
    39901 ttattttgtc atatatgaaa gctgatacaa taccagctac tatactcaat aaaaagttaa
    39961 ttaacatata aattcacctc cttcctatat tggaatttgg cgtttatatg agaaaatcac
    40021 ccttcgcttt tcaattttat tttccttgct aaaaatatta taacatataa ttcttacata
    40081 ttttacctat atattacttt tttaaagatt cgtctatata tttctgtata catctactct
    40141 atcatcgaat agaatttata acttagattt gatataataa aaccatagtt attcaatata
    40201 ttttaaacat gaataatact tataaaggag tgtacatata tgaatatcaa atcagctttt
    40261 ataagaaaaa gaggggaaaa atttcatgta tatgtggagt acatagaaga aacaactggc
    40321 aaaataaagc aaaaaagtta tggaagctat gaaaagaaaa aggatgctga aaaacattta
    40381 attgaaataa agtctactat aaacaataat aagtttataa ctccaaacaa aacaactctt
    40441 gtagagagat gctataagta cataatgaca aatgaaaaaa attggtctcc ttatacagtt
    40501 ataaatagga aatcttgggt taagaattat atagaacctt tttttaaaga tacaatattg
    40561 atagatgtaa accctagcct tcttcaaatc tttatagata aaaattttga tggttctgca
    40621 ccatcaactg caaaagttag atataatttt ctatcttctg ttttaaaaga agcttatagg
    40681 ctaaaagaaa tatctgaaaa tccttgcgat tttgtaaaat tgccagctca aaatgttaca
    40741 tatgaaattg aaatatataa tagagaagaa acgctattgt taatagagaa gttgaaagat
    40801 agtttgatag agatacccat tctcttaatg ttgctgttag gtttaagaat tggagaggta
    40861 gctggtctta gatggtctga tgttaattta gataatagta taataaatat taatcaaatt
    40921 ctcatatatg caaatagcaa aataactttt aaagaaccaa aaactgcaaa atcaaaaaga
    40981 gcgttatctg ttccaaaaga gttagttgaa aaactaaaaa tagaaaaatt aaaacaaaac
    41041 aaaatgaaat tacaaggtac acttataaat gaaaataact tagtatgtct aaatacaaat
    41101 tttaatgctt ggataccaac tgcattaagt aaaacttttc ataactttat taaaagaaat
    41161 aatttaagaa atattagagt acatgattta agacacacaa acgcaagttt gcttttgcta
    41221 ggaggtacaa atatgaaagt tgtttcagaa agattaggtc atacagatat aaaaataact
    41281 atgaataggt actctcatgt gttagaggaa atggacaaag aagcttctga taatttaagt
    41341 aaacttctat ttaaataata aaaactgact gtcagttaaa tgtcagttga acctaaaaaa
    41401 ataggttttg tcagttaaat gtcagtcagt tagtttataa taatttcttt taaataccgc
    41461 taaaactgca cgttttaata cttatttatg tttgtttata tgtatttatt attctttatt
    41521 aaaataatat cctgctcctc ttattgttat aattttctga ggatttttat aatctttctc
    41581 tattttcttt cttaatccat tcactgcaac taccacacta tagttatcct ctaagtattc
    41641 attcatccat acatgttcat atatctcttt agtagtcaaa gtcttttcag gattctctat
    41701 aaaaaagttt aacaacttat tttctgttgg agtcaatttt attattttat cttctaactt
    41761 tattactcca ctagttctat tatattcctc taattttgta ttattttcat tattatttac
    41821 atatgagttc attcttctta aaatagcttt tacttttgcg tataatattt ctaaatttag
    41881 aggctttatt aagtaatcgt ctccaccaga atttaaagca tttaaaatgc tttggtcttc
    41941 atttatataa gtcatataaa caattggaca agtagttaca tttctaattt ttttacaaag
    42001 agtccatcca tcaccatcac ttagaattat ttctaaaaat attaaatcat atttattgct
    42061 aaatattttt ccaatagctt cttcacaatt atatgctaaa tctatactta ttcctttttc
    42121 ctccagaaat ccttttagct tcttgcaatt atccttgtct ccatctatta tcaatatctt
    42181 agtttgcatt ataatcactc ccaaatatca ttattctttt atattaaata agctattatt
    42241 ataaaaaata tagacttaat tttattgtaa aaacagttcc ttttcctagt tctgaatcta
    42301 caaagatttc tccaccatgc tctcttataa tggatttgac aatatatagc cctaaccctt
    42361 gcccttttgg ctcattattg tctctttctg taaagaatct gtcaaatacc tttggtatat
    42421 tttctggagg tatacctttt ccattatcag aaattttaat aattgcaaat ccatcttctt
    42481 tatccattga taaaattatc tttccatcaa atggagtaaa ggtcaaagca ttataaatta
    42541 aattctcaaa tgctcttgta agcatctctt tatttatctt tgcatatatt tttttctcca
    42601 atacgtaagt ttcaaagttt acaccacatg cttgtgtatc tggcaaatta ttttcatata
    42661 tctctttaat aaaattattc agacatattt tttcactaat accaatgctt ttatcttgtg
    42721 atgtaaattc ttgcaaacta cgtactctat tttgtacttc atttgacttt tgatttatag
    42781 cataaagata ggttcttgtc tcttcatcta catgaatatt accaaccttt atcaaatcta
    42841 taaatgcttg tatagccgaa acaggtgcct ttaaatcatg tgctacatct gccaagaatc
    42901 ttttacgttc atttaacaat gttgtaagtt cattagttct cacttcaact tcatgttcaa
    42961 gattccttgt aagtttttca ttctctttta ttattattct attcctgttt atcatcataa
    43021 cagaaaataa aataacaatt acaaacccac aatattctgt ttgccaccct gtaaatatag
    43081 gctcaaatct attactcgta actgcatcaa ataatatgct aattccaaaa acagtgttta
    43141 ctgaaactaa aatataagaa cttatatgct cagagcatgt ctttttatat attgaaacca
    43201 gcgaagacat taatatatat ataaatacaa tatatttata tatgtctatt aacttcccat
    43261 atatgttgac aaagtacata tcatatggaa gaagaataat tggtatcact atagatatga
    43321 tactcattgt aattcctaat ggtaaaatta aaaatctgta cactttattt tcttctattt
    43381 tggatatact tccatttata caaataatac ataaaactac cataaaatat gacaaatctt
    43441 caaccgcata tgtaaagctt accaaaggca gtcctaacaa atgtacaaag ggataggata
    43501 catggatggc aaagaaaaca cacatacatc caaaatataa gtaaatttta cttttgtttg
    43561 aaagaaacca tacagacaat gaaaatatag ctattgcaaa agttgtgaag caaattatac
    43621 tataaaaaag taatcgataa aacatcatat tacttatgtc ctttgtttct cctaatgcag
    43681 gcggataata caaaccacta taataatgtg aaaaattcga tacattgatt ataatttctg
    43741 tatttttctt tgcaataaaa cttactactg aatttttaat tttgggtcta tatttttctg
    43801 ttcctacatc tcctaattta gaaaccattt ctccatttat ccatacttca cttgcggaaa
    43861 aaatctcagg aagctctaaa cttagtagct tatccttgcc ttcatttttt attctcatac
    43921 gataagtagc attcccatat ggtgactgag tcccctttct catagaaaaa tctggatatt
    43981 gacctataaa aacatattct ggttttatat cttgctctgt aaaatcattc ggtttaaata
    44041 ttttatgatt ataaaactcc cagtcatcta tcaaaaagac aagtttacct ttagtgaaag
    44101 tatcattatc tatagttaaa attccattac ttgctctaat agacttatat gtatatttat
    44161 tatcatattt atacagaaga tttaatcctg ttattgccaa cagagagact atcaatacaa
    44221 tgagagttcc ttttagtata ttatactttt tcattgtatc aaccacatct ctttcatagt
    44281 agtattaaat aaaacaagaa ctatttatta attacagtat ctcacttatg gtacaaactt
    44341 atatcctttt ccccatacgg tttgaatgta ttgatgctcc ctgcttaaag aattaatttt
    44401 ttttctcatc tgtgcaatat gaacctgcac tgtatgttga tctccaatat caggcatttt
    44461 ccaaacttgg ttatatatct ctatcttcga aaatacttgt tctttgtttt cagctagaaa
    44521 caataatatt tcaaattcca tagtagttaa atctatactt tctccatcaa aagttacttt
    44581 tcttgaattt acatttatta ctaacgctga aaactttaac acattaggaa ctgatgtaat
    44641 atttttgtac tgcctcatac gagcatatat tctaagttca agttctttta aactataagg
    44701 ttttgttata taatcatccc ccccagctaa aaatccccga actttttctt cttcatgctc
    44761 ataattagat aaaaatatta taggtatgtt aaccttttct tttatatcat tgcacatttt
    44821 atatccatct atatctggca atgaaatatc caaaattata cagtctaaaa ctatactctc
    44881 tactatatcc atagcatctt tagcagtctc tgatgtatat acattaaaac ctatattaga
    44941 aaaataaatt tgatttatgt ttaaaatatc aacatcatca tctacaacca gtaaattata
    45001 cattacacca ctccattcaa aggtatttta attataattc caagttttat agttattaca
    45061 aattataatg ttttattttc atatataact ggtattatta ttttataata tctttacaaa
    45121 ctagactata atacttaagt atttttgaat aattcttgaa tattgtatta aacattaaga
    45181 ttcattatta aatactaatt aaagtctccg attgttggag aatggagctt aaagtcattt
    45241 tcacgtataa atttattatt tttctagaaa cttgtctatt ggctagtttg agtattgatt
    45301 tatatgattt atatggttat aatataaaat aaattgtatt attttggtaa atatattgtt
    45361 acaaaaggtt tatattctgc atctcaatgt ttataaaaac tttattttct tattataata
    45421 ctattattct tttaaaaaaa tttccctttt ccaacaaatg gctactttaa aagccaaaat
    45481 tttacctatc aataaaaatc ttaacgacta atactcttat tttcaaattt tgcaacatat
    45541 ttgttaattt taaaacaaaa aaagactatg caatataaaa attatattca tagccggttg
    45601 caccactaac tccatatatc tctagatgcc caataataag atatacttct cagtttctaa
    45661 aatattttta ttaaaaatct tttatatatt ttcataaatt tcttttttat tctaacattg
    45721 taatattatt ttgtcaatat ttccatattc ttagagataa gatactaaaa tacctatttt
    45781 atctaaagat atttatattt ctacattcaa taatagacaa taccctgcaa gttaaagcat
    45841 catttttatg agcttatctc acagggtatt atatgtttac tttatattat aactcttgtt
    45901 attgattaac tttaataaat cctttttctg aaacaagtgc ttgtccttca tcacttaata
    45961 tataatctat taaagcttta cctttttctg acaaaccatc ttctttatta actaatataa
    46021 atggtcttga tattggatat ttattagcta ttacatttgc atcagttgct tcaacatcat
    46081 ttatttttaa actatttact ttctcatcaa catatccaaa tgatatatat cctatagcat
    46141 tttcatttcc ttcaactgta gtttttatat ttcctgaacc atcacttatt tgtgcatctt
    46201 tagttagttc ttctgaatca aaccctacaa tttcttggaa tccatctcta gttcctgaac
    46261 cttcttctct tgatactatt actattggtg catcatttcc tcctacttct ttccagttag
    46321 taatctttcc tgtatatata cctttaactt ggtccattgt cagatttttt actgagtttg
    46381 cagtattagt tatcaatgct ataccatcta ttgctatttt agtttccttt aatccagcac
    46441 ttttttcttc atcttttaaa tctcttgaac tcattcctat ttcagatgtt ccttctatag
    46501 cattttttat acctccagaa gaaccaatct gttgtacttc tacactaaca ccttgatttt
    46561 tttcttgaaa cttttctccg atagtttcca taagaggacc tattgaagtt gaacctgaaa
    46621 cagaaacttt gcttgaatct cctgaatcac ctgaacctcc tgagttagaa ctacatccta
    46681 ctgctaaagc tccaactaac attgtactag ctaaaagaac gcctattttc tttttaaaca
    46741 ttttacttac ccccgcttat attttaattt tttgttgtca tttttctata ttttaataat
    46801 acagattctt tgttaaatga atattagagc agggttaagt aaatgtaaat attgttaaat
    46861 acatgtaaaa acttaatttt atatgattta ttcttaatta gattaacttt ttaaagttac
    46921 tttaggcaga tataaacata atacaactca tattatcaga agtcttcatt gaagctatca
    46981 acctatcaca tcctataatt ctttttctag tttattaact aattcagcaa atacatttaa
    47041 ggcttcctct atagattctt ttttcgtcat atctacacca gcagatttta attgttctag
    47101 tgggtattct gaccctccac tttttaagaa gtttatatat ctatctactg cagtacttcc
    47161 ttctgaaagt atctgttgac ttaaagcact tgcagcagaa aaccctgtgg catacttata
    47221 tacatagaag tttgaataaa aatgaggtat tctcgcccat tctattccta tttgtttatc
    47281 tactattgca gattttccgt aatacttttc atttaatttg tagtatatat tagtaaattc
    47341 atcagcagta agtggttctc cactctctac cctttgatgt acaatttttt caaattcagc
    47401 aaacatagtt tgtctatgaa ctgtagttct aaattgttct aaataataat ttaataagta
    47461 tatagtttca tccttagact tagaattttc taataaataa tttataagaa gtaattcatt
    47521 taatgtagat gcaacttctg caacgaatat cttatagtcc gaatataaaa atggttgact
    47581 tgttcttgaa taatgactgt gaacagagtg ccctaattca tgaattaaag tgaatagaga
    47641 attaaggtca tttttatagc tcattaatat ataaggatgt gaatcataac ttccccaaga
    47701 ataagctcct ccttttttac cttcattatc atatacatca atccaccctt catcaaaagc
    47761 actttttatg accttcaagt attcttctcc taaaggttta agtgcctcta atatgatatc
    47821 ctgtgcttta tcatatggta tatccatgtc aaatttatct gtcaaaggaa catacaaatc
    47881 atacatgtgt atttcattca atcctaaaaa tttcttttta agctcaacat atttatttag
    47941 attaggaata cttttatcta tagctgatat aagattattg taaacatcta aacttacatc
    48001 atctgaaaat aaagacgctt ctattgctga attatacttt ctagtttttg cgtaaaatat
    48061 ctctgatttt attccaccat aaagcgttga ggcaaatgta tttttatatt tctcatatgt
    48121 tgaaaattca ccttcaaaag cgtcttttct aactctacta ttcttacttt ttataaataa
    48181 agagtagttt gaatgagaaa gcttaacctt attcccctcc tcatcttcta tctcaggaaa
    48241 ttccatatct gcatatgcta acatatcgta tacattttct ggaattgtcg ataattctga
    48301 tgtagctgca agtatttctt cctctcgttc actcaaagta tgtggtttat ctctcaatat
    48361 atcctttata tattttgcat aaaaggatag cttctcatct tttaagtatt cacttaactt
    48421 attctcttct atcgccagta tttctggaac tatataagaa gtagccacac ctaaatcagt
    48481 agataacatt tctgtcttag tggctcttcc ttgattatca ttaattctag tatcttcatg
    48541 ttgtttcata tgagtatata catataaatt ttgaacaatc ctagatgcat tttcagagat
    48601 atttaatact ttatataagt tctccttact ctcagaaagc ttccctttat attcttttac
    48661 ttcttggata agatttttta ctttacttat gtctttttca atttcctctt tactggagta
    48721 catcttatct atcttccatt tgtacttttc ttctattgta cttctatcag ttcccatatc
    48781 atacacctct attttaattt gttttatatt ttattcactt tacaaaatat attgtatata
    48841 ttttagtcca aataatctac atttattact ccttctatca tatttatttc tttcataacg
    48901 tcgtgtaagt gtattctctt agcataagat acaaaaaaat taaccttcca gtattcttca
    48961 ttacgacatt ctatttccat atttcttacg ataagttgat ttttttcaaa tttatcatat
    49021 aaaaaaggta tagaatccaa attgcttaat tttaatatca atacattctt ttcttggcta
    49081 aataaaaacg tattgattat cctaagcaca aataatgcta tcattacaaa taatccacct
    49141 agaattgcca attcataata accatatcca actgctatac caatacatgc agttgcccac
    49201 atcccagcag ctgtagtaag cccttttact ccacctttat tttttaaaat tgctcctgca
    49261 cctaaaaaac caattccaga taatacttga gcaggcattc ttgctgggtc agcatttgta
    49321 tacaaattat aatcaaagaa taattgaatc gaagttattg aaacaataca agaacctatt
    49381 gcaattagta tatgtgtcct aaatcctgca aactgtctta ttttttctct ctctaagcct
    49441 attattcctc ctattattag tgctaatagt aaccttattg ctatctcctt acttcctata
    49501 tcaatcataa tataaaaccc tcctgactta attacaactt atgtcctaca gataatttac
    49561 acatcagaaa tactatttta tatcttaata ttattattaa tatatgtaaa atatagccat
    49621 ctgttaattt ttgatacaaa aaaacccaca actgtgggct atttgcttag gtacatgtat
    49681 ttatttatta atatatctat tttcttactt gattctacta actcttcttg aaatatttct
    49741 ggatattcta tgtatgtatt tatctcttcc ctaactgctt caatctcatt ttttaacgct
    49801 tctaattcat tgttcgccat aataatcacc actattacaa atttgtatat ttttatatac
    49861 attttatcaa acaatattat attttgtcaa tattatgtca ataaaactta ctataaaaat
    49921 gctttaattt aggtattgta tacctggata ctaagcctac aaacatacca gttactaatg
    49981 atgccattaa cataaatggt aagtacccta tagcaagtat attttctatg attaaacttg
    50041 caactattaa ttgacctata ttatgcccta ttgcacctat aatgcttatc cctattagtg
    50101 ataaatcttt tattttactc gctaaataca taaataaaag actcagataa gctcctccta
    50161 tactaaatag tagatatgat aaagggcctg caaatattga tgacaaaatt accctgatag
    50221 taacaattat aacagtatct ttgacaccta acaacattaa tgaaattaaa gttataatat
    50281 tgctcaaacc caattttgcc cctggaaata taactataag aggatttggt atatatgtct
    50341 ctaatacata taaagctaaa ctatacccta tcataagacc taaaaatgtc atttttctag
    50401 ttttcattaa gttaactcct ccaaatttta tctatcatct tacattataa tacaaaatat
    50461 ataaaaacac ctaatttaga ctaaaatatt tttaatctat ttagaataaa tattagttat
    50521 ttcaatgtgc aattacctct ataaatcttg actttttgta tcatacttgt tacaatttta
    50581 atgataatga atttcattag gaggtgttaa taatgactgt ttacgattta aaaataggag
    50641 aaaaaggatt tattaatagt atagatggag atattaaatt atctaaaaga ttgcttgcac
    50701 ttggatgtat aaatagtact gaaatagaag ttaaaaaggt tgccccactt ggagacccaa
    50761 ttataattag atttagaggc tttgaccttg cgattagaaa atctgatgct aaaaatattt
    50821 ctttacaata atagaggagg agctacccgt gattaatgta gcattattag gaaacccaaa
    50881 tgtcggtaaa actactgttt ttaatctact tacaggttca aatcaatatg taggtaactg
    50941 gcctggtgtt actatagaaa aaaaagaagg atttcttgga aaagagataa aggttgtcga
    51001 tttacctggt atatatgcaa tggatacttt ctcaaatgaa gaaaaggttt ctaaatcata
    51061 tttagaaaat gaagatgttg atgtaatagt aaatgtagtt gatgcttcta atttatcaag
    51121 aaacctttac ttaacaactc aacttatgca atttaacaag cctatagtta tattgcttaa
    51181 tatgttagac attgcagaat ctaaaggagt taatatagat gccaaaaaat taagtgaaga
    51241 acttggagta attgttgttc caattatagc aaaaaagaaa aatggtgttg ataggataga
    51301 agaagagatt aagggagcta cgcaaaaagc ttttatatat cagaaaaact ttggaagtga
    51361 aacagaaaca tataaaaaat tagaatccat tttatcgaga tgtacaaaaa ctacatatac
    51421 acaaaagaaa tctatcagtg ataagataga caatatagtc ttgaatccta tattagcata
    51481 tccgatattt attggtgtat tgttcctctt atttaaattt acctttgact gggtcggagg
    51541 accactacaa gagggatttg ctagtcttat agaggcttat atttctgcac ctgttagtga
    51601 tatgttatct aactctagcc catggtttaa atcacttata gttgatggaa taattggagg
    51661 tgttggtggt actttaccat tcttcccttt aatatttaca ttattctttg gtatatcgtt
    51721 gtttgaagat agtggatata tgtcaagaac agcattttta atggataaag ttatgagaaa
    51781 ggttggtcta tctggtaaag cttttatacc tatagttatg ggtatgggat gttcttctcc
    51841 agctataatg gcaactagaa cattggaaag cgaaaaagat agaagagtta cagctcttat
    51901 tgcaccactt atgacttgtg gagcaaagct acctatatat gcactatttg tagctatatt
    51961 ctttccacat aatgctgctt tagtaactac atcactgtat ttggtgggta tagttatggc
    52021 aattgtagtt gcattattct taaataaaac agcctttaaa acagaggctg aaccatttat
    52081 attagaatta ccagaatata gaatccctac tatagatgca cttattaaaa atacttggaa
    52141 taaatcaaaa ggtttcttga taagagttgt tactgtcatg tttgcaatgt cagtcgtaat
    52201 ttggggactt tcttcattca atgtttttgg atatacagaa gatataaatg taagcttctt
    52261 ggctactcta ggtcatatta tatctcctat attcaaacct cttggttttg atgattggag
    52321 aacttcagtt gctattttaa gtggtcttgg agcaaaagaa atcgtagtaa actctttaaa
    52381 tatactatat ggtaatctaa ctgttgtctt accaacaata tttaatggag ttactgcata
    52441 tacattcttg atatttactg ctttatatac tccatgtata gctgcacttg caactatgaa
    52501 aaaagaatat ggcaataaaa tgatgtttac atcatttgca tatcaattta tactagcatg
    52561 gataatggct tttatagtta aaagtatagg tggagtacta tttatgggta attcaataat
    52621 agaagctttg ataggtggaa taattatagt ccttgcactc tttattctgt ttaataaatt
    52681 caagtctaca aaaaacggaa atgtctgtag tggatgttct atttgttctg gatgtccaag
    52741 tgctaattct tgctcaagta aggaagacag caataaaaaa aataatgcac actaatatat
    52801 aaatatttta gtttaaaata agataaaagt gggtgtctca aaattaactt tttagttcat
    52861 gaggcactct tttttataga aaatcaaata tattttcatc atttcaacag taaaaaagag
    52921 cttatctagt aaaaaagagg cttatctcta ttttgagaca acctcttttt ttgttactta
    52981 ttcagtttct tgctacctaa cttcatgtgg aacctagtat cttttggaac acttgggtct
    53041 acacctctta atgtaggtat ttccccacat attagttgta taggtactat aaaattaaat
    53101 gtttgatagt aaggattatc tgtgatatca atctttaaat ttttatcatt ttctgtaaca
    53161 tctctaccta tagcaaatac attttccgtc caaccagaaa gtacatcaat catcttagtt
    53221 actctaggtt cttttcctgt atctagtata aatatagtag aatcactatt tatagcatta
    53281 tatataccat gtataaactc ttcaaactca tacccagtta caggtattct cattgtttcc
    53341 aataatttta atgctgcttc taatgtatct ccatatatat cagagtgacc tattattctt
    53401 atttctttag aattaactaa cttttctttg tttctttcaa tccattgttt agagagctta
    53461 tacactgctt caaatctatt aattgcgtct aatatcttat ttatttcctc attgtacttt
    53521 tcactagata ttataccttt ttctctagct atttgaagac ctaataacat taagtttaac
    53581 tttgtacaat aatatccttt tgttttagct ccagattttt cttcaccaca atttactgtt
    53641 aatatataat cagaaatctc atctattaaa gcattcttac aacctgccat agatgctatt
    53701 ttacagccct tatcttcagc taacttcata gcgttgtatg tagaataact actcccacct
    53761 tgagatacac ctactactag ggtattttca ttatcaaact tgaatgtatc ttcagttatc
    53821 ataaatggat acattttcac aacatctata tctaataaat tttgtaaata tggttgtact
    53881 tgcactcctg agtgatagct agtacctgaa cctgttatta taatcttttt tagatttgtt
    53941 ctcttaactt cattaaataa gctatctgca ttagatataa tttctctcat tctaactggt
    54001 gtttctaaca tataatcttg tattgtcatt ttatacacct tccttatcta tatattcaat
    54061 aaattataat taatttctat aacaaaccta tagcatgtaa agcgattcct gttactatag
    54121 tgcctatagt taattttgtt acacttacct ttctctttaa catgtagtat attacaaatg
    54181 caaaggttaa aggtaacata ttaggtatta ttttatctag tatatcttgt aaaattactt
    54241 ctgcaccatt taagttgaat ttcagaggag tagttatact caacatagtt gcaaccattc
    54301 ctcctacaac gaataaacca acagttgtag ccatagacat tattttctcc ataagtccct
    54361 ctctttgtat tctttcaaga gaatttaccc ctacttgata accatactta agtccaaata
    54421 ttctaagggc aaatgctggt atattgtata atagtaagaa taatagtggt ccaaagatat
    54481 taccttcttt tgcaagtgat actcctactc ctgctgctat aatcctaaat gttccccaga
    54541 ataatgaatc accaattcct gcaagaggtc ccataagagc tgttttaaca gcactaattg
    54601 aactttcatc aaattctgga ttattagcat tctcttcttc catagctgct gttataccta
    54661 atatagcagg aactactact gtcgttgtat taaaaatctc caaatgcctt tctattgctt
    54721 ttgcttgatt ttctttctta ttgtatagct tctttattgc aggtaacatt gaataacagt
    54781 aacctaagtt ttgcattctt tcgtaattaa atgcaccttg aagagcaaat gaacgccaaa
    54841 acatacttct taattctttt tttgttaatt tattcaaagt caatctctcc ttccgttaaa
    54901 gtattgattg ctaattgagg attttgatta ttttctttgt tattgatata aaagtttagt
    54961 acaaatgcta cacatactcc aaataaagct atagcagtta tatctaatcc accataagat
    55021 gctaacaaaa accctataaa gaaaaataca gacatctttt ttgaaagtaa catatccata
    55081 agcaatgcaa aacctactgc tggcaacaat gttcctgcaa cacccagtcc gtctaatata
    55141 actttaggta ttgcatttat caatgctgta actttatcag caccaatcat aattgctaaa
    55201 aaggttggaa ctgatgtact taaaaagaat aatattactg gcaaccacat cattatggta
    55261 actttcttaa aatcagcttg tttagcatag aaaactgcct tttgagaaaa gtacgaattt
    55321 attattctaa ctaatactcc aagagattgt gctaagactg ctactggtac tgctactgct
    55381 aaagcaactt ctgcaccatt acctgataat atagcaaaag ctgtacctaa tacacctcct
    55441 gttactacat ctggaggagt agctgcacct attcctgcaa ttcccatcca aattaattca
    55501 agctgtgcac caatcataat tcctgatttt atgtctccta aaactaatcc tactaacata
    55561 cctgtaacta ttggcctatc aagcatagat tgaccaaata tacgcccatc tagtatacca
    55621 actcctgcta taatagctaa catagtagct tgtactaaca tattgaacct ccattttaac
    55681 ttagtttaat ttacattaaa ttacattaca tttttgattt tgttttgatt ttctaataac
    55741 tcatttacat aaacaattcc attttttgct tcctctaatc ctgatttaac tattagtttt
    55801 gtatcctcct caactaataa aagtatagat aaaagcattg gtaaatttac tcctgtcaat
    55861 aattcaaatt tgaacccttg taaaagtaag tttgtacata tgtttgccac acttccacca
    55921 aaaacatctg ttaagacaac aaattcttca ctatctttat tatttgataa tatacttcta
    55981 atttctttct cagcatcatc tgctgattta tccgttgtca tacaaaaata ctcaatatta
    56041 gttaatgaac caattattaa tttacttgtg tcaacaagct ctctggacaa ttctccatgt
    56101 gttgcaatta gaaatttttt cattctcttt ctcctaaatt attttattaa atctgatagt
    56161 tgtttctttt tgtctctagg taacattcta aattctatat caatccctaa atcacctatt
    56221 tgtttaaagt tatttatatc atcatcatca acagcaacta aatctgtaag tttcttcttc
    56281 ccttcctcat atcttaatcc tcctacattt atttctttta atactcctcc tgaattttta
    56341 gctacaacta aagcatcttt ggtatttttt actaacacta atacattgct tttctttgaa
    56401 gaagcaaatt tttctactat ttctccagat tcctctaccc cccttatata taaagttaca
    56461 ttgctaggtt tcgctaagtt cagtgacata gctttcattt ggtcattttt tgcttcatca
    56521 tttgcaacta gtattacatt tatacctagt gatgttgccc atccatatgc aacctgacca
    56581 tgtatcaatc tgtcatcaat tctcatcaaa gataccattt tccccaaatc ctccatcata
    56641 cattaaaatt tttattgttt atactctcat ttatctataa aagcaaaaag tatgccaaat
    56701 actggcatac ttttttaaat ttcattcact tattttgtaa tcttgtattt tactgtggta
    56761 gaccatagat aatattataa atatatccta tctctgaaat tggtatttgc acactataac
    56821 tcttttctag cacactaaat gcatttttta ttagttttac ttcactatga tggtttttga
    56881 ttaacaaatc catatcagta tactcttcaa tttcagtttg tctaactaat ctttcaatca
    56941 tacaacttaa gtgaacatat aaagaaatct ttttatcatt tgaaagtttt ttcttttgaa
    57001 tattttctat agcattaatt ccttcttcta ttctctctat tatctttttt gaatctaaaa
    57061 tagttagact atctattact ctaattaatg aaaaattctt tacaatagaa tcatttatgt
    57121 tctttatttt ttcattttct actactctgc taaatattct aaaaacatca tcttctcctt
    57181 ttcctgatat tatatcctct aataatataa aatctacccc attcacattt ggattggcag
    57241 ttcctactat agccatcaca tcatacattt caaaaactgg ttctttatct cccatagaaa
    57301 caagactatc ataatcataa ggtattattt tgacatcaac tattccaatt aaactttcgg
    57361 aaagtagatg ttggattttt atagcagtac ctatccctgt aaaacaagat gatattatag
    57421 catattcttt atttttttga ggataataca attgtatatt aggtttagtt tcttcatcca
    57481 taatttctgc aatctcttca atgtttttac cttgcattaa taaatttcct acttctagtg
    57541 cagaaatagt ggatatttgg tctataaata acattgggct atttatctta ttctttaaat
    57601 cttcatatat aagattaaga gagcccatat caaataacac aattaaacca ttctttatcc
    57661 tatctccctc taggtatcta gacatttgtt ttgatacatc cgcaatagta gcatctatag
    57721 gcatatcaat agctgtaaat atatttttac ccaacatccg attacataca ttcgccatac
    57781 tactggcagt agcatatcca tgtgctataa tagtcgcatt aacttgatta aatttcattc
    57841 ttataattgt tgatttaata aatataatca ttatgattat atctacatta ttaagataca
    57901 tatccaatct attcgttatt aatgttgata ttttacttat tatagagtat tctttgtaac
    57961 tctctttttt taaatatatt agtaatttat tttctaaagc tttatactca tcagacaatt
    58021 gataagattc gtccttcaaa taaagataag acgaaaatgc caatacatta ttcccatcat
    58081 attttatatt gttattaact tctaaatttc taaatatatc ctgcattatc gatattataa
    58141 gctccataag tacgttatta ttttcatcat tatttgaaaa gactaaattg tccattagtc
    58201 taatcattag attagaactc tttttactaa atttttcctt tgttatttca tttttttcta
    58261 gtctatcaaa gtaatctaaa cattttatga aactttgata gattatattt cttttatttc
    58321 ttccatttga catttcaata ctaaattgtt taggagaaaa gataatgctt ttgcttttac
    58381 cactttggaa ttgaaatata tctacatttt ctatataaag atttctagga aggtcttgaa
    58441 gagtaacaaa aatatttcca tttcttcttt gattttttgc ataagctcgt ccacatgcat
    58501 attttattac atttttacat tcacctatat tccccttgaa ttttatcttt aaaatcgcat
    58561 ttaatgtaat ttgagttatc tctataggca aaccaagtac cttactttct tcataaataa
    58621 aatgttctac aatctttctt ttttcaatag tccctctttc ttctatattg ggaacataaa
    58681 tatttatggg tattcttctt aaaaaggttt gtaaaaattc agatagactt tctgttgtag
    58741 caaatattaa tcttacccta gcttttctaa ttactccatt ctctccaatc ctagaaaacg
    58801 tacctgtatc cataaatgta aataactttt cctgaccttc actatttaat ctatgtactt
    58861 catctaaaaa taaaacacca ccatctgatt gttctattag acctgctcta gattcatttg
    58921 ctccagtata tgctcctttt acatgcccaa ataatagact agacaataac tccaaattat
    58981 tataatactg cgcacagttt aacacattgt atggagaatt tttttctaat acattactgc
    59041 ttacactata tttgtatatt atcttggcta aaaaagtttt tcctactcca ctttcacctg
    59101 tcagcattat tgtaagtccg ccatttggat aaagtacagc ggtttttatt tgttcgatta
    59161 cttcttttaa gctattatca tatcctatga tactattaaa cacagtctct tgctttaata
    59221 atttacactc atttttaagt tcatctaaat tttcataaat actttttttt gtaggtccta
    59281 ctacatcatt tattttattt ttaggtaaaa aaataacagg tctactgttt atctttataa
    59341 gattatcaga ttttactaaa tcatttaata atctacttga ttgagttcta cttaaattaa
    59401 acttttttac tatatcttct gttattatgc cactttcttt tttaagttca tcaaagttgt
    59461 taaataatac acattcattt acaaaactta aaatttcatc cctcatgttc aacctccaat
    59521 acaaatatca ataatactat aatattagta tatcatttat atatttatga aaaagataat
    59581 tatattccta tcttgattct cttttgtaag tttgtaaaat caaacttttt ataagtataa
    59641 aatctagatt ttttaacata atctcaaggt tcttatcttt ccatatatct gacatagaat
    59701 ttatataaga atatatttat tgtatttatt ataataaaaa gtaaaacatt ataggtattt
    59761 taaaacttat tagcgaagaa aatatttgta acttttatta ccttatgaat attatttcaa
    59821 atttaaaaag gctataaata aatttatttt taaataaatc tacttataac ctcacaataa
    59881 tacttataaa ttttattttt tcacaatgat atatctcaaa tatagttaaa aatatatcta
    59941 tttaattatt aatatttaag taataaatta tcactcaatc attaaattcc tttttatagt
    60001 aaattactct tattttactt ataccaaata catttatgta tagatataaa taagcactaa
    60061 aaaaacaatt caagaaacca tatagtaaac ttaattacat ctgttatttg ctcatatctt
    60121 tagacttatc tatacaagca acaatagatt ttataactgc tgctctaaag ccttcttctt
    60181 ctaatacctt aacagcttct atagtagttc ctgctggaga acaaaccata tcttttaatt
    60241 cacctggatg ttttccagtt tcaagaacca tcttagctga ccccattact gcttgagatg
    60301 caaatttata agcttgttgt cttggcattc cagctaatac tgctgcatct gccatagctt
    60361 ctataaacat aaatacatat gcaggagaag aaccactaat acctgtaact gtatccatta
    60421 aatattctga tactatttct gcctcaccaa aagattcaaa cagagcctta atcatattta
    60481 actcttcatc tgttacatta ctatttttac aaagagctga cattccttcg ccaacaaggg
    60541 ctggagtatt aggcattact cttactattt ttttatctga acctataaaa ctttccagac
    60601 tttctatagt tttacctgct gctatagata ctattatttt atttgcatct attaattctt
    60661 taagtggagt taaagccttt tcgcatatat taggtttaag tgctactatt accacatcag
    60721 aatttgtaac agctaccttt gaatcagttg tagtatttac tccaaatttt tcttttactt
    60781 tatctaaggc aacttggctt aaatctgata cagttatatc ttctgccttt actaatcctg
    60841 actgtactat gccacctatc attgcacttc ccatattccc tgaaccaata aatcctaaag
    60901 ttttcatatc aaatctcctt ttttctatta gttttatttt aaagtagcta aattgacttt
    60961 attttaactg cttaaaatta tcttattttt ataaaatata accttattat aactataata
    61021 aattattctt ttgtatgtat tacttactaa tatgtgtatg atttactaaa tataaattta
    61081 acgatttttt cattaaactt aacgagctaa cttcctattt agaaagttag ctcataatta
    61141 atctaaaatt ttaaaaatgg aatcaaacca tttatacttt attatatcag ttacgatttt
    61201 atatagtaag aagttttatt tacaagcaaa cctagaatgt ttgctcagtt cttcctatta
    61261 tctcatcttg tagtgttcta cttaaattat tgaaatggtc actatatcct gcaactctaa
    61321 ctattaagtc tttataatcc tgaggattct tttgagcctc taacagtaca tttttatcaa
    61381 atacattaaa ttgtatatga tgaccatcca tattaaagta tgctcttact agattagcca
    61441 tattatctaa ccctttttct ccttgaacta ctgatggtgc aaatctttga tttaataatg
    61501 ttccaccagt ttttatgtgg tccatcttag cacaagattt tataactgct gtaggtccat
    61561 tagtatctcc acctttttca ggtgaaattc cctctgaaac tggttttgca caaagtctac
    61621 catttggact tgctcccata atttctccaa agtatatatg acaagttgtt ggtaacatat
    61681 ctactctgta ttctccacca caaacagttg gtctaccagt tacctcattg taatatgcat
    61741 tgaatatctc ttgcattatt tcatcagcat aatcatcatc atttccatat ttaggagtct
    61801 tatttgaaac taaattaagt atagcttcat atccttcaaa attagcatca agtgctttaa
    61861 gcaatgtatc catatcgaat tttttcttat caaatacatt atattttatt gctgttaaac
    61921 tatctgttat agttcctata ccaacacctt gtatatatct agtgttgtat cttgctccac
    61981 ctctttggta atccttgcct ttttctatac aatcatcaac tattgtagac ataagtggac
    62041 aaggcatgtg ttttgcacat atattttcta tcacagcatt tccttctatt tttatgtcaa
    62101 tcatataatg taattgcttt ttaaatgctt caaatagttc ctcataagat tggaaatttc
    62161 tagggtctcc agtttcaata cctatttgtt ttttactaat tgggtcatat ccattattta
    62221 atgttaattc aagtattttt ggaatgttca tatatccagt tagaacatat gcttctttac
    62281 caaaacatcc agtttcaaca caaccactgg ttccaccaag tcttgcatct tctatagttt
    62341 ttccagcttc cataagctct tgaactattg cttcagtatt ataaaatgct ggttgacccc
    62401 atccttttct tgatatttca caagctctct ttaagaattt ttgaggagtc tttttactta
    62461 tttgaacatt tgaactaggt tgtatcaatt tcatttcatc cataacatca agtattatat
    62521 aactaacttc attaacacca tcttgaccat ctggatttat tccaccagtg tttatatttg
    62581 caaaatctgt atatgtgcta ctttctttta atgttatacc aacttttggt ggtgctggct
    62641 gattgttaaa tttaacccat aaacattcaa gtaattcttg tgctctatct ctatctagta
    62701 ttccattttc tacatctctt tcatagaaag gatttagatg ttggtcaagt cttcctggag
    62761 taaaagcatc ccatatatta agttcagtag taacccctat atgaacaaac caatacattt
    62821 gtatagcttg atgataagtt tctggtttgt gagctggtac aacatcacaa gtttcagcaa
    62881 ttagtaataa atcttttttc ttagcttcat ctgtttcttc ttctgccatt tgtcttgctt
    62941 tctcagcata tcttttacct aatataacca ttgcatcaca agctattgcc atagcttcta
    63001 aatctgcttt tttattatat gcatctaaat cattaagaaa atctagctct tttaatcttg
    63061 cttctatgtc tttctttaaa tctaaaaatc cttttttata tattgtatct ccacaaactg
    63121 tatgtcctgg agctctttgt tccataaact ctgtaaacat acctgcttca tatgctgcta
    63181 accactcagg tgtcattgca tttattatct tatctcttgt ttgtctcttt ttccaaaaag
    63241 gaattatttc ttctttatgt atttttctag cttcttctga tacgctaaaa tttattatat
    63301 ctctattgtg cattacttct aaatcttcca ttgtatgaca acaaatttct ggatatgttg
    63361 gtgctccatt aggagaatca cctttttcac ctactattaa ttctccatca tttatgctaa
    63421 gagttctatt ctcaatataa tgcttaaatg ataaagcacg aagtacagga atctctacac
    63481 ttccctcata ttttttataa gcttctgtca taagtactgc tctttcaata gatatgtgtg
    63541 gttctgcatt aatactttct tctcttaatt ttttagttct ctcaaaagtt cctcttgcca
    63601 ttttattacc ctccaatctt agtattgaaa ttacttgctt caaacagtct ttttatttcc
    63661 tctaattttt cttctgatgg tctttgtagt tcttctcctt catacttttt ataaatttta
    63721 tcatacttgt gtttaccaat attatgatat ggaagaagac ttactgcttg tatattcaaa
    63781 ggctttaaaa actctatcat ttttttaacc tctaaatttt catcatctac atttactcct
    63841 acaattaatg gtattcttat atttatgtta gcccctaatt cacttaaaat cttaacattt
    63901 ttcagtatta agtcatttga ttttcctgta aattttatgt gcttatcttc atcaataagc
    63961 ttaatatcat ataaaaacaa atctgcacat ttagcaactc tttcataatt ttctgacttt
    64021 gcatagccac atgtatcaat tgctacatgg attccctttg atttgcacat gtttattact
    64081 ccacaaatga agtccatatc ttgagccatt acttctccac ctgatagagt tactccacca
    64141 cctgattctt catagaaaat tctatccttc tctatctctt taaccaaatc tcttacagaa
    64201 tactcttgac caacaatctc tcttgcattg tttacacaat agtctaagca agtttcacaa
    64261 aactcacact tatcttggtc taagcatatt tttgtttcac ctttgtaaat tgccttatga
    64321 gggcatatat ttatacatgc ttcacatttt gaacatcttt cttcattata caaaacttgt
    64381 tttgtatatg tttgactctc tggattatga caccaaacac attcaagtgg acacccttta
    64441 aaaaatactg tacttcttat ccctggtcca tcatgtatgc tacatttttg taagtttata
    64501 actaatggat tcatgatttt tcacccctaa ataatatcca ttccattttt tcatttttat
    64561 gcaattcatc ttcatagaat atacctatga aaagcatttc tacaattaca taatactata
    64621 tt
//
LOCUS       DAYAPA010000004        61555 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.004, whole genome shotgun sequence.
ACCESSION   DAYAPA010000004 DAYAPA010000000
VERSION     DAYAPA010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 61555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61555
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.004"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            246..767
                     /locus_tag="ACGI59_000202"
     CDS             246..767
                     /locus_tag="ACGI59_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFG9738016.1"
                     /translation="MDIAKLILNPARLRILQYIRLYGSVRTSDIVKYLNDIPRATVYH
                     HVKILEENNMIEVIKENRVRGTIEKVYALKEYTTSMEGETFVALSTAFYVGLMQEMNE
                     YFSRKEQDHKKDNVFFSSALLYMSDNEYENLLKSIADLLKPYIEQKPKSNLKLRKLSI
                     ISSPPVADNDKTK"
     gene            801..1577
                     /locus_tag="ACGI59_000203"
     CDS             801..1577
                     /locus_tag="ACGI59_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFG9738017.1"
                     /translation="MKFHEFGNKNRPHIMLIHGGGNAWWNYLRQAEVLSNKYHVILPT
                     LDGHGEEYETTYISTEDTADKLISYIDENCSGHLFALCGVSLGGQIVMELLSRKPNIT
                     KKAIIDGSICYPRPLMARFCIAGIRFFGGLLFSEKACRFQIAMMPKLLPENMQYPDKI
                     KAYYMTDMPHVRKETFYNMYRTYMMNYTLKENVKKTTAQVMYWYGEKEMKCVKKSAKL
                     FQSYVPSCEIYEAKSYNHGYLSLYLPNEWLEIAEPFFQKE"
     gene            1919..2872
                     /gene="gpr"
                     /locus_tag="ACGI59_000204"
     CDS             1919..2872
                     /gene="gpr"
                     /locus_tag="ACGI59_000204"
                     /EC_number="3.4.24.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GPR endopeptidase"
                     /protein_id="HFG9738018.1"
                     /translation="MISVRTDLALEASEMCEKSQEGSSIPGVKIETKELENCIVTKVE
                     VIDRQGAEIMNKDIGKYITLESNLMKFDDDESREEMISYLKEELVDIFGQDKNKKTLV
                     IGLGNRNITSDALGPKSVSKTLVTRHLFKNYNKDYDDDFTEVSALSPGVMGVTGIETS
                     EIVKSLVEKVKPDRVVAIDALASRKMERVNSTIQISTAGISPGGGVGNTRKSLTKETL
                     GVDVIAIGVPTVVDAATLTIDVLDMAIDNLIAQSEETESFYEMLKKLKEEEKYHLIKD
                     SLDPYDKNLIVTPKDIDDTIENLSIIISEGLNRSLHPGRLV"
     gene            2890..3909
                     /gene="spoIIP"
                     /locus_tag="ACGI59_000205"
     CDS             2890..3909
                     /gene="spoIIP"
                     /locus_tag="ACGI59_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061783 - peptidoglycan muralytic activity
                     [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein P"
                     /protein_id="HFG9738019.1"
                     /translation="MFKKCIKVVTLTFILVCILPGKSLALNQDDFLKFLVNSSYPEAK
                     VEGNDTENKKNNKNKETSKENKEESKEGNTKSKDASKVDNKKESEKEYIKLYVGKENV
                     PDIESKNSDTTETNTTSSSDYKDDLRVTKENPRILIYHTHGCETYSNSPDGNYHSRDK
                     KNSVMEVGSALTSALDSKGWGVVHTTKYHDYPSYNNSYASSLKTIQSILPKYNSVDIA
                     IDLHRDARDLTNPATKEKDHLKYTTMINGERVSKFFFVVGGKNTNRKQLRALAEDITA
                     FAEKKYPGLVSPIVEKDYARFNQFAVKNHMLVEIGNNATSVEESKATTKYLAEILDEY
                     FKQKN"
     gene            3940..4191
                     /locus_tag="ACGI59_000206"
     CDS             3940..4191
                     /locus_tag="ACGI59_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9738020.1"
                     /translation="MSRLEKNIESKSKLRKYKYIMRILFLITMIICTSVCVFIVDSNA
                     KSMLGMKQYDFPSIKFDITKFDFDIPNFDIHEIIKKFNK"
     gene            4473..6278
                     /gene="lepA"
                     /locus_tag="ACGI59_000207"
     CDS             4473..6278
                     /gene="lepA"
                     /locus_tag="ACGI59_000207"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFG9738021.1"
                     /translation="MDNKQSRTRNFSIIAHIDHGKSTLADRLIQQTGLVSERDMKSQL
                     LDNMDLERERGITIKLQNIRLMYKAKDGNEYYLNLIDTPGHVDFNYEVSRSLAACEGA
                     LLVVDAAQGVEAQTLANVYLAIDQDLEILPIINKIDLPSARPEEVKNEIEDLIGLDSS
                     EAPLISAKTGLNIEDVLEDIVKNVPPPKGDNEAPLKALIFDSYYDAYKGVVAYVRVFE
                     GTVKKGMTIKMMNTNKKFEVTEVGVMAPGQTELSELSAGDVGYIAASIKDIRSCRVGD
                     TITDSNNPTEEPLPGYKKATPMVYCGIYPGEGEKYENVRDALEKLQVNDAALEYEAET
                     SAALGFGFRCGFLGLLHMEIMQERLEREFNLDIITTAPSVIYRVTKMDGEVVMIQNPA
                     NLPEPSEIKMIEEPIVKGDIIVPKDYVGVVMELCQERRGNMLNMEYIDDRRVMLHYDL
                     PLNEVVYDFFDALKSRTRGYGSLDYEVKGYVASTLVKLDILINKEQVDALSFIVHETR
                     AFPRGKAMCEKLKGEIPRHQFAIPIQAAVGNKVIARETISALRKDVLAKCYGGDISRK
                     KKLLEKQKEGKKRMRQIGSVEVPQKAFMSVLKLDE"
     gene            6427..7764
                     /locus_tag="ACGI59_000208"
     CDS             6427..7764
                     /locus_tag="ACGI59_000208"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HFG9738022.1"
                     /translation="MINIEIPKKVDYIIKELEKNGYEAYIVGGCVRDCLLERVPNDWD
                     ITTSARPEVVVELFEKTIPTGIQHGTVTVMIEHEPFEVTTYRIDGNYSDGRHPDSIEF
                     TNNIVKDLSRRDFTINSIAYNSKTGLVDPFNGYEDIQNKYIRCVGNPVDRFEEDALRM
                     LRAVRFSAQLNFKIAEGTKQSIHKKADLIKNVSIERIQTEFNKILVSDSSKLNLLKST
                     GLLKFIIPEICELEDVTQHNPYHIYDVQKHTLIATEVIEDELYLKLTMLFHDLGKKVT
                     KTTDKNGVDHFYTHSRESVKIAKKILKRLKYDNYTINKVLILIQYHDYRIEPKRKIIK
                     KLLNKLEDVELFEDLIKVNWADTLAKNPKYAKQKILNLIECEKEFKHIINQKECFNLK
                     DLAINGKDLISIGIKPGKDIGYILNKMLEIVINNPELNEKEILKEKALNIYTF"
     gene            7997..9310
                     /locus_tag="ACGI59_000209"
     CDS             7997..9310
                     /locus_tag="ACGI59_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFG9738023.1"
                     /translation="MHTQLQKTIGLSAALSTVVGMVIGSGVFFKPQAIYTTTNGAPGL
                     GIIAWLLGGFITITAGLTATEISAAIPKTGGMMIYIEEIYGEKLGFLTGWMQTVLFFP
                     GTSAALGVIFAQQASELLGMSPNNMANVLPIAIGVILFLALLNIIGSSLGGKVQTVAT
                     IGKMIPLILIIVFGFIKGQSSEVLNPFVGDGVNASNALGQVLIATLFAYDGWINVGAI
                     SGEMKSPEKDLPRAIVGGLSLVMAVYIIINIAYLWVVPASELATVTSPATLVATRLFG
                     NIGGKVITVGILISVFGTLNGYLLTGSRIPYTLAEMGTLPASKTLLKVNSGGSPVNSI
                     LLITVLACVYALSGQFNLLTDLTIFSIWVFYVLTFIGVMRLRRQKPDLHRPYKVPLYP
                     IIPIIAILGGLFVIINQILTSTVISLGGIFITLLGLPVYYYMKKR"
     gene            9482..10657
                     /gene="hemW"
                     /locus_tag="ACGI59_000210"
     CDS             9482..10657
                     /gene="hemW"
                     /locus_tag="ACGI59_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFG9738024.1"
                     /translation="MLGLYVHIPFCVKKCKYCDFNSYKMDIDSKKRYIEDLKIEMELY
                     SNKLYKDNKYKNKECCSLNKNDKITSIFVGGGTPSILTSDEIREVFISIKEMFDIDEN
                     AEITIECNPGTLTLEKLKTMKEIGINRLSIGLQAIQEKHLNFIGRIHTYEEFEKNYKD
                     ALSVGFKNINIDLMYSLPNQTLCDWKETLEKVVHLNPTHISAYSLILEEGTELYNMYE
                     SNKFELIDENVDIEMYEYTINYLKSKGYNQYEISNYSKEGYNCEHNILYWECEHYIGI
                     GAGASGYINENRYNNVESLEDYHLSLVKREKPIQENEILSEKDMIEEKIFMGLRMNKG
                     IKFEDFKKKFGIDFREKYNKQIEMLLARKLINQSFEGIQLTQKGREISNSVFIEFME"
     gene            10760..11779
                     /gene="hrcA"
                     /locus_tag="ACGI59_000211"
     CDS             10760..11779
                     /gene="hrcA"
                     /locus_tag="ACGI59_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HFG9738025.1"
                     /translation="MELNERKLNILKAIVKDYIETAEAIGSRTISKRHDLGVSAATIR
                     NEMADLEELGYLIQPHTSAGRVPSEKGYKLYVNSLMSKSELDDNDKILIEQCMNHNIN
                     HIKELIHETSKLLSQLTNYTTVAVTKSLINQSVIKHIQLVAMNDNNILLIVVTDKGDL
                     KKANLTTNVYLDQSKLNLISDNLTRKLLGKSITDLDDNLIAFIKYEISEYSGLIDELL
                     NALNSNMKEEDFSLSLNGATNIFSYPEFNDVLKAKSFLNMLEKKETIADIIKSKGIQK
                     DNLNIIIGSDNDCELAQDCSIVTATYNVDRDLVGRISFIGPTRMDYARIYSIINYMSL
                     LINRK"
     gene            11809..12429
                     /gene="grpE"
                     /locus_tag="ACGI59_000212"
     CDS             11809..12429
                     /gene="grpE"
                     /locus_tag="ACGI59_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFG9738026.1"
                     /translation="MDKKNEQQEVREENDTSINQESETQVELEEEVVNEECETSSEKT
                     DEKEVDDENVTDINSKLAEKKLQDELDELNDKYQRLQAEYANYRRRTQQEKETIGVFA
                     NEKIITELIPVIDSMERALDACEDKEDTMYKGISLVHKQLIDTLVKFGVEEIEAESKE
                     FDPNLHLAVMQESVDGVEANQIVMVLQKGYKLGTKVVRPSMVKVSC"
     gene            12521..14368
                     /gene="dnaK"
                     /locus_tag="ACGI59_000213"
     CDS             12521..14368
                     /gene="dnaK"
                     /locus_tag="ACGI59_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFG9738027.1"
                     /translation="MGKIIGIDLGTTNSCVAVLEGGEAQIIANSEGMRTTPSVVAFTK
                     DGERIVGEPAKRQAVTNADKTITSIKTHMGTDYKVNIDGKSYTPQEISAIILQKLKSD
                     AESYLGQTVTEAVITVPAYFTDAQRQATKDAGRIAGLDVKRIINEPTAAALAYGMDKL
                     DQEKKILVFDLGGGTFDVSILEIGDGTFEVLATAGNNRLGGDDFDQIVIDYLAEEFKK
                     AEGVDLRNDKMALQRLKEAAEKAKKELSSTMSSNINLPFITATAEGPKHLNIDLSRAK
                     FEELTRGLVEKTMEPTKTALQDAGLSTGDIDDVLLVGGSTRIPAVQEAVKKFIGKEPH
                     KGINPDECVAAGASIQAGVLAGDVKDLLLLDVTPLSLGIETMGNVMTKIIERNTTIPT
                     KKSQIFSTAADNQTAVDIHVLQGERSMAYDNTTLGRFQLTDIPPAQRGIPQIEVTFDI
                     DANGIVNVSAKDLGTGKEQKITITSNTNLSEAEIEQKIKEAEMNAEADKQKKEKIEAF
                     NQAESTIYQTEKTLNELGDKISSGEKEDIEKAIADLKAVKDNQDATAEELKKATDEVM
                     TKFQKVSQEMYQKAAQEQQAAQGAEQAQDNGPKDDNVVDADFKEVDEDK"
     gene            14579..15733
                     /gene="dnaJ"
                     /locus_tag="ACGI59_000214"
     CDS             14579..15733
                     /gene="dnaJ"
                     /locus_tag="ACGI59_000214"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFG9738028.1"
                     /translation="MSTKRDYYEVLGISKGAEAQEIKKAYRKLAMKYHPDRNPGDKEA
                     EEKFKEINEAYEVLSDDTKRKTYDQFGHDGLNGQGGFGGQGGFGGQGFGGFEDMFGDI
                     FGDMFGGSFGGGRARRRGPQRGADIRQSVTISFEEAAFGKKMSIKVNRSEECEECNGT
                     GAKPGTSKKTCSTCNGTGQVRTVQRTPFGNIASSRPCSACNGTGEVIESPCSKCHGTG
                     NTRKVKTIEVDIPAGIDDGQMIKLSGQGEVGEKGAPRGDLYIVVNVKSHPLFTRDGND
                     IYFEMPITFVQATLGDEIEVPTLDGKVKYSVPEGTQTGTVFRLKEKGIPRIRGNSRGD
                     QYVKVVVEIPKKLNDKQKELLREFAKECGSNVHEKKKTFGQKIEDMFKKK"
     gene            16699..17634
                     /locus_tag="ACGI59_000215"
     CDS             16699..17634
                     /locus_tag="ACGI59_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFG9738029.1"
                     /translation="MYYIGVDIGGTGIQAGVVDNYGKIIFRSECKTVIEKGFEGILND
                     IKIMIYKLLEDNKLTMSDIKSIGFGVPGFINKEGLVTCVNLKWNKKAFNKELKRRFPD
                     VEIHGENDATVAALGEAKFGSMKGANVGVLYTLGTGVGGGIVINQKVFSGAHGLGSEI
                     GHQIIGENYFNCNCGNNGCVETFCSATAIIKYSQKLIEEGEKSRILDLAEGNLENVNA
                     KMVFDAYRENDLVAIKVINRFKEYLAKTFANTINSLDPEIISIGGGISKSSDIILDGI
                     EDLVRKFVLYKTEDIATITCATLGSDAGIIGAAFL"
     gene            17670..19043
                     /locus_tag="ACGI59_000216"
     CDS             17670..19043
                     /locus_tag="ACGI59_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFG9738030.1"
                     /translation="MGKTNARRTLFLIAIGSGTMLNPLNSSMISLALHSIQNDFHISF
                     TTVSWIVSAFYLASAIAQPVSGKIGDLIGRKVLFLSGLMLVILSASIVPLVQSFFILI
                     FIRVIQAIGTSTLYPSGVALIQNNIKERQSSALAVLTIFASTTAGLGPTLGGLLLDLG
                     GWHAIFLVNIPVVLVSICLGYFLFPKEVKEKKSIKETLKNVLSRLDIIGILLFSIAMI
                     FILLFLLSMKESFNLEQLVFGIILMCLFIWHELRTKLPFIDIKLFVSNPKLSKVYLQF
                     IILNFFNYILFFGLPSYFQDALHYSAKSTGLFMLFMSGIGIFISPLTGKWIDKSGTRF
                     PLVTSSIFMFISAVLLSVVFIHIPVIGKGIILSLAGISYGVGNVALQSSMFEESPRDS
                     IGTASGLFQTSRYLGSILATVLLGMVFEVNITSEQFQILGYVMVVLGTISFLLNFILK
                     KELRKVE"
     gene            complement(19231..19998)
                     /locus_tag="ACGI59_000217"
     CDS             complement(19231..19998)
                     /locus_tag="ACGI59_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HFG9738031.1"
                     /translation="MEELKDFLERQLNKKINIYPYENQKLDASSHLVTFNNAIYVIDF
                     LDKNNLDNLKGNFYLIYQSHIDFEYLKNIFYNLFEDINIIQHNGFFIVNSKYNLDINV
                     TTQNIIETETYQSTYIFYLGELDSKADFDFRLQLCSDLLPHIIKDNAENKFLNLFDFI
                     RYKTLDLINEDNILNKLIDFNKIKSIDEELLYTGIKFINNDLNISKTSTSMFLHRNTL
                     VYRLEKINEILGFDLKNFENAMIFYLSVKSYFLYKKI"
     gene            complement(20028..21137)
                     /locus_tag="ACGI59_000218"
     CDS             complement(20028..21137)
                     /locus_tag="ACGI59_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9738032.1"
                     /translation="MSEVILKNISKLYSNGFNAVKNINIDIKDKEFIVLVGPSGCGKS
                     TTLRMIAGLEEISEGELYIGDKLVNNIEPKDRDIAMVFQNYALYPHLSVYENMAFALK
                     LRNLPKDEIDKKVKEAAKILDLLPLLNKKPKTLSGGQRQRVALGRAIVRNPKVFLMDE
                     PLSNLDAKLRTAMRTEITKLHQQLGTTFIYVTHDQVEAMTMADRIVVMKDGVVQQIAT
                     PQDVYDYPANIFVAGFIGAPQMNFIDVILIEENDEIYAQNEHIKLKLNKEKHYDLIKD
                     NYINNEVVIGIRPEDIHVEDTFIKSNPDTCFKSRIEIKELMGAETYAHLKLGENTITI
                     RFDSKNRINVGDDLTLSVDNSRVHIFDKETTLAIR"
     gene            21395..22132
                     /gene="cas6"
                     /locus_tag="ACGI59_000219"
     CDS             21395..22132
                     /gene="cas6"
                     /locus_tag="ACGI59_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HFG9738033.1"
                     /translation="MSRISIFMESCNGKFPNENSMLSVSFIKNILNAENKDFAKNIFN
                     YGEERKNNKQIKDINTAIYIPNLIRSKNELLVDGDIIFNISFYNYSMFSKLYNGILKV
                     KELEYKGYRFKIKNIKIHKEHEIKENGVIFKTMSPIIVKNKEGKYLDVEDSNYIECLN
                     YIANLTLESIRGSGLRKPLEFIPINFKKRVLKEKIRGFKVREYYYINAYAGTFFLKGD
                     MEDLNALYKMGIGYRRTENAGMVDILK"
     gene            22137..23612
                     /locus_tag="ACGI59_000220"
     CDS             22137..23612
                     /locus_tag="ACGI59_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9738034.1"
                     /translation="MREIKAKFYRSGDIVRDIGIVYFYELLMDLKNELEKNDYKLKFT
                     CELNRNYLSLESAEYIDPKYISDYILENRLFKVFKNGKKETLEKIFSNIDNLTYVNFI
                     EELDKSSATAKQKEDIKKDFKNRRFPYVRNSGKYGLNTSLENMETNVKRIVDTVIKSN
                     IEIDEIDKLDLTQYEEKDSVCNVCNINKTTKLDIDLRERKDSKYNFLFRGAEKSGFKR
                     SGQVESNICFECEFFNLMCLLYINLKRPMVFAYTNDLRELAFLNHKIMLKRKMYSDKS
                     FYKKLLHEKISSIRLYSFDIDTNKGIILKFDSIIEYKELLKKIELIDIIDNYNFSREP
                     GNTRNLGKRMIDNGNLSNLKELLLDNISVLKQSTGTSREMDFVSSSYNIGLYIKLCWI
                     VDGGEEYKMKNYMESNLIYSRVGKDLFNKMTDESRRKNFSMKVIQLLKSNDRTQLFQT
                     IMHVLVSNGILIGEGFVEGLMQSSELELNTNVGLFIQEIMK"
     gene            23632..24645
                     /locus_tag="ACGI59_000221"
     CDS             23632..24645
                     /locus_tag="ACGI59_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DevR family CRISPR-associated autoregulator"
                     /protein_id="HFG9738035.1"
                     /translation="MNEKRVKGVTLTILTESPVPLSYDQGYGNYTPIKKEQYREKIHA
                     KTSIATITYDLRRMLHQEYGWNLSNIVFGKKGNIYPSIKKNVGCVDENGLETDVFGYL
                     IPLKDKGSISKASPLRIIPFRSLNPYKGSTQLITNRGFLSSEFGRKYYDEKEENEVPR
                     DENFPTSQALAIEETLSDYYVYTITLELDRIGVVEVEDGKLLLPEERKFMSKELREKA
                     VKDILDAITVFTRNIKHSSVLLKPLAVIGGAFDKVVPFFWDDVDYNADSGEINLEGVI
                     ETIESYSLKESNTILAINDRLKISNKKELNKFNLSKYPVKEIKNLADRLEIGEDNMWY
                     LKE"
     gene            24661..25458
                     /gene="cas5"
                     /locus_tag="ACGI59_000222"
     CDS             24661..25458
                     /gene="cas5"
                     /locus_tag="ACGI59_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas5"
                     /protein_id="HFG9738036.1"
                     /translation="MKVLQCKLKQPTAHYRDPKVFQNEYISTLNLPSKTTIMGMITYL
                     CDRRLNSDIDIGIIGTHHHRELEFSRGENIDFWNEYIKMGKGKDKEKFLLKGNYYDYY
                     KEHKAQNSILNYEVLKEVELTIFISCKDDEELEFIRKKLESPCKYANLGRKEDFVIPS
                     EKGCFVKEVKLKEVIPMNTRDAIKENIKLKNTYVRVDLRDKENVETIINQGVLIALPH
                     KYKDLEANRDDRVYEFCHYIYVDNDGIYPKNIKVNVCIDTKEVFTWL"
     gene            25485..27893
                     /locus_tag="ACGI59_000223"
     CDS             25485..27893
                     /locus_tag="ACGI59_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HFG9738037.1"
                     /translation="MKNIMQKEILAKSYNLRTNQQEQTLVEHIKDLFEVLESILELNL
                     YSDKDVEILKICCALHDLGKINSIMQQKMEINNKISYSCSEEERKKLESDKKSLKKIA
                     RHNIFSGAFLKDILEKMNLSEEDKLYIYKSIMLHHGNYEDYMRLSISKVQEEIYEYIE
                     KGILEKEEFNLKDIESYINSILNVDFKFGEDVLDYDYIDKLSESMFIDSDYNNGQIDD
                     SVLNYRKFKYILYKGMLNLIDHSASSRQKGIKFYNDFTDEEIDNMILNEIYKSQGNLE
                     KNNIEFNTIQKRLRELSGRNVLTVAFTGSGKTAADYRKTFKRKFFLVPNKISAESFYR
                     KNIFQNKNDLDTRSSNDYIGLLHGDINLYSENEDNGEHDFVLTLRDIDLSINFCKPCV
                     IATVDQLLLSMFKFPGYEKIFAAVKNASITVDEVHLLEPKMFLILIYFMQFACKYLDV
                     DFHLMTATLPKSYKEQMINKGIIFQEESNENVTDKGEIVFIESNKEEEICEGKDVKVS
                     FIKEKEIKSIVEDALENKQKILIIKNTIDSVNRTYEFLKENLSDKYGGVDIDVLHSRF
                     KFKDKKEKYSKILNGEGDIWISTQSVEISLDLDFNIIISDLATMDSLIQRMGRCNRNN
                     KYEYGNFYILPSEDKIYDNKLKSTTKNILKDILKTNSIFTMGIRKTILDDYYDNSVVK
                     KYFEENFISCDKEIKNIYGVNKEIFDGLDLIFNFEPYKNIVDSKKEAAKIFRDVDVSY
                     KIILEEDFYKEDRELQQDSIQVSGFIFNRLYYYGLISKVEGYMVLKSSSKFEYNSTVG
                     LILK"
     gene            28334..29281
                     /gene="prmA"
                     /locus_tag="ACGI59_000224"
     CDS             28334..29281
                     /gene="prmA"
                     /locus_tag="ACGI59_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFG9738038.1"
                     /translation="MNNWIEVTIKTTTEAVEPITNILYEQGAGGAVIEDPKDFLFQKK
                     NELDWDYVEEEVFKKNEEDDVLIKTYVSEEKNVMEFVEIIKQKVLGLKDFGIDIGEGS
                     VSLDQVNEADWANAWKAYYKPTKVGQRVVVKPTWEDYAMQEGDLIIELDPGMAFGTGT
                     HETTSMCIRELEKYVNKDSKVFDIGCGSGILAIAAAKLGAKEVVAVDLDEVAVKVAKE
                     NVLENKVEKSVSVMHGNLTDVIKDKADVIVANIIADIIKILAKDVQNFMKEDAIFISS
                     GIILDKVEEVKESLIENGFEIVEVQKLGEWSAIVSKLKK"
     gene            29300..30058
                     /locus_tag="ACGI59_000225"
     CDS             29300..30058
                     /locus_tag="ACGI59_000225"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFG9738039.1"
                     /translation="MDRFFVEKNNINLQDKTCTIEGEDVKHISKVLRCKLGEKLEICD
                     KNNNEYICEIMNIDKSIVNLEILEKVDINRESELKVRLYQGLPKAPKMEIILQKLTEV
                     GVEEIILVQTKRSVVKVDDKKEDKKFERWERIIYEAAKQSKRGKIPKLRGVLSFKEAL
                     EDMKKNSVNICPYENERTVSIKHALKKCDSNIDSVGIFIGPEGGFSEEEIEQIQKNNC
                     NVVSLGPRILRTETASVVASTIALYELSDLGGEK"
     gene            30060..31358
                     /gene="mtaB"
                     /locus_tag="ACGI59_000226"
     CDS             30060..31358
                     /gene="mtaB"
                     /locus_tag="ACGI59_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0061712 - tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HFG9738040.1"
                     /translation="MKKVAFYTLGCKVNQYETEAMLELFEKDGYEQVNSEEYADVYVI
                     NTCTVTHMSDRKSRQYIRRVKKKNPDAIIAVVGCYSQVSPEEILDIEEVNLVMGTNDR
                     RKIVEEIKKINSSKKVSTVDDIMKVKAFEEIEISQTNGKTRAFMKIQDGCDRFCTYCI
                     IPYARGRVRSRDIDSIVDEVKKLANNGYKEVVLTGIHVASYGKDLKDRDIKLLDVIKQ
                     INQIEKIERIRLSSVEPILFTDEFVNEVLKMDKVCPHYHLSLQSGCDETLKRMNRRYT
                     TLEYKTIVDRLRSKMPDVAITTDVIVGFPGETNEEFKKTYEFLKEIELSQMHIFKYSP
                     RKGTPAATMENQVDPQMKHFRSEQLLNLSKVNFNKFATKFIGRELDVLFEQNIEGNKY
                     EGLTSNYIRVVVESDKNIQGQILKVKINDVKDEYVEGILL"
     gene            31401..31751
                     /locus_tag="ACGI59_000227"
     CDS             31401..31751
                     /locus_tag="ACGI59_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HFG9738041.1"
                     /translation="MDCIFCKIANGEIPSTKVYEDDRVLAFNDLNPVAPYHILVVPKK
                     HYDSLIDIPDKEMDIVSHIHVVINKIAKEKGFDQTGFRVINNCGSDGGQEVKHLHYHI
                     LAGKKLPNYEAGQN"
     gene            31914..32093
                     /gene="rpsU"
                     /locus_tag="ACGI59_000228"
     CDS             31914..32093
                     /gene="rpsU"
                     /locus_tag="ACGI59_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFG9738042.1"
                     /translation="MSEVRVRENETLDSALRRFKRQCAMSGIMSEVRKREHYDKPSVK
                     RKKKAEAARRKNAKK"
     gene            32123..32566
                     /locus_tag="ACGI59_000229"
     CDS             32123..32566
                     /locus_tag="ACGI59_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HFG9738043.1"
                     /translation="MSLKQKLQEDLKSSMKNKDTVRKSVVTLIRASIKQYEVDNRVEL
                     DEDGIIDVIAKQLKQRRDALVEFEKAGREDLIKETEGEIEVLKEYLPQQLSEEELEEI
                     VKSTISEVGATSMKDMGKIMSVIQPKVKGRADGKLINKLVKQNLQ"
     gene            32677..33174
                     /locus_tag="ACGI59_000230"
     CDS             32677..33174
                     /locus_tag="ACGI59_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HFG9738044.1"
                     /translation="MIISVKEIRNFLINIFIPLIIGYLSSILTMIISGTDISTYYLQL
                     TKPSFAPPAFIFPIVWTILYILMGISSYLILRKGYNLPKVRDAIFYYGLQLVLNFIWS
                     ILFFGFGLRFTALIDIIIMILVSIIMISKFSKIDKRSGYINLIYLMWLVYAGFLNYFI
                     WFINK"
     gene            33367..33930
                     /gene="raiA"
                     /locus_tag="ACGI59_000231"
     CDS             33367..33930
                     /gene="raiA"
                     /locus_tag="ACGI59_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFG9738045.1"
                     /translation="MQIIVSGRQMKLTDGIKGYVDGKLSRLEKYLDPESEVKVTVSAK
                     KDRQKVEVTIIPINGQIIRAEDVEDDLYAAIDIVCDKLSRQVVKYKTKVKDKVQNNKS
                     IRFENLDFIDNSSEFDDYDYDDEEDENIVIERRKKFNVKPMSSEEAILQMELVGHNFY
                     MFRNQDNFEINIVYKRKAGGYGLIEQD"
     gene            34143..34379
                     /locus_tag="ACGI59_000232"
     CDS             34143..34379
                     /locus_tag="ACGI59_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YabP/YqfC family sporulation protein"
                     /protein_id="HFG9738046.1"
                     /translation="MLEISTDLQVNQPVITVTSNTFISIENYLSILEYEVDLIRIKTK
                     VKTIKISGDKLSLKYITDSEIGIKGIIYNVEYVD"
     gene            34392..35735
                     /gene="yqfD"
                     /locus_tag="ACGI59_000233"
     CDS             34392..35735
                     /gene="yqfD"
                     /locus_tag="ACGI59_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YqfD"
                     /protein_id="HFG9738047.1"
                     /translation="MISFIRGYYVIVVEGVGLEQFLNHLIRNGISVYNVTRIKNTKME
                     FHIDRQDIKDFKNVYRGSKFDIKVKQKTGVPFIIKRVYKHKGMWICALVSLFLLMSTS
                     QFVTDVYIQSPEGIKKEALRNELYKVGVRPGVYKKSIDRKEVRDHMMSKFNDVAYLSI
                     NVKGTNIFVTVTKKAESLKSTDQSNYCNVIALKNGIIEKVIPRSGKSVIKSGDIVQKG
                     DVLLNGANTKSIPEVWASTFYESTKKASYVDTVNKKTGEKKNIYTLSFYDKEFTMRKN
                     IKYKDYVVENKEKKLSIGNYTFPIKIKTSTFYETKKVEVKRNKEELKKELSEKALKEL
                     EYIIPASARIIDVKHNFKVNKNMLEYLITVQTSENIAKIYPLSKSEAERFIKEESKPD
                     EGEEEVPSNPEKRPLNDIRNEFDEDNKDNKDQNNSDENNSNQNSNNSQNNNSNNN"
     gene            35674..36789
                     /locus_tag="ACGI59_000234"
     CDS             35674..36789
                     /locus_tag="ACGI59_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFG9738048.1"
                     /translation="MKIIVIKIVIIVKIIIAITTNKIKLKILGGLIQLIIQKKFTIAD
                     GNFERELFGNFDENVKLIEKTLNIDVILREGNIILIGEEKNVDSALKLMNELHQTVSN
                     GKHLDKQSISYSLSLLLEGSEQKIKELEGTIVITQRGKAVQPKTLGQKEYIKLIENND
                     ITFGVGPAGTGKTYLAVAMAVKAFKRDEVSRIILTRPAVEAGESLGFLPGDLKDKVDP
                     YLRPLYDALFEMLGADKFNKYLERGTIEVAPLAFMRGRTLDNSFIILDEAQNTTSEQM
                     KMFLTRLGFGSKAVVTGDVTQTDLPQNKKSGLIQATEILKGVPGIGSIMLTDRDVVRH
                     ELVQRIIRAYEKHDKREEFKKEERKKAKMQEKTFKRK"
     gene            36846..37307
                     /gene="ybeY"
                     /locus_tag="ACGI59_000235"
     CDS             36846..37307
                     /gene="ybeY"
                     /locus_tag="ACGI59_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFG9738049.1"
                     /translation="MDLILDDRQDKLEVSEELIEKIKDIIIECLDYEGYDDNYEVSLS
                     FVDNKEIHELNREYRGVDRVTDVLSFPLLSDDFEDVELEEESLGDIVVSLERALEQSI
                     EYNHSFEREVCFLICHSMFHLLGYDHDTDENTKEMREKEEHILNKLNITRE"
     gene            37313..38032
                     /locus_tag="ACGI59_000236"
     CDS             37313..38032
                     /locus_tag="ACGI59_000236"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - ATP-dependent diacylglycerol
                     kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFG9738050.1"
                     /translation="MKPEKTRQGIIKAFNAAIEGILYTFKFERNMKIHYLGSVAVLII
                     SLFFNFSKLEMIMLLMSICLVVVAEMFNTAIEKAVDLVTDEYHVLAKIAKDVAAGGVL
                     VAALNSVVVGYILFYDKLTDISGILIYKIRESELHITLICILLVLIAVVVVKALTSTG
                     TPLKGGMPSGHAALAFAIATAITLMTERVVASTLAYIMAVLVAQSRIEGKIHTFWETI
                     AGALLGVLIAILVFQLGMFYN"
     gene            38151..38552
                     /locus_tag="ACGI59_000237"
     CDS             38151..38552
                     /locus_tag="ACGI59_000237"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFG9738051.1"
                     /translation="MDNIELLRLAEDARQHSYAPYSGFRVGAALLTKSGKVYTGCNIE
                     CASLGGTNCAERTAIFKAISEGDKDIYKIAIASDNSENNEQTYPCGICRQVIIEFGSD
                     IKIITGYTKGEVFEHSIKDLLPNYFSGNDLK"
     gene            38616..39509
                     /gene="era"
                     /locus_tag="ACGI59_000238"
     CDS             38616..39509
                     /gene="era"
                     /locus_tag="ACGI59_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFG9738052.1"
                     /translation="MFKSGFVSIVGRPNVGKSTLMNNVVGEKIAIMSDKPQTTRNTIQ
                     AVYTDEEMQIVFLDTPGIHKPKNKLGEFMVKAATEAFKNVDLILFVVDDSKKIGPGDR
                     KIIEDLRSVKTPIILVVNKIDQLDQKDELFDIIKMYDREGIFKEIVPISALKGKNTDT
                     LIKVIQNYLEEGPKYFPDYMITDQPERVLIAELIREKVLHYLNDEIPHGVAVEIEKMK
                     ARNDKEIVDVSAVIYCERDSHKGIIIGKNGRKLKGIGKSARQDIELLLGSQINLQLWV
                     KVKENWRNLQNYINNFGYNDK"
     gene            39521..40900
                     /gene="mgtE"
                     /locus_tag="ACGI59_000239"
     CDS             39521..40900
                     /gene="mgtE"
                     /locus_tag="ACGI59_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HFG9738053.1"
                     /translation="MDRKLDASQDLLYEVKSLIDNNKVLELRELIEEYHIIDIFDIME
                     NLEEDMKIQLFEVLPLDMASSILEEGSVEFFISILSKLDVEHSKNILELMSLGDMADK
                     LSELEEEEREHIINLLNQENADYVKELLFYDEDSAGGTMTTGYISINKDMTALEAIDH
                     MREEAEEAETIYYIYVVDDEEKLVGVLSLRELIIARDANIVEDLMSENIISVYVDEDR
                     EEAVRLVSKYNLIAIPVVDRQEKLKGIITVDDIIDVMEEEATEDMYKFAGSSEHEREV
                     AEKENPTLREQIISALRGRLPWLIITLVGGLLASLILSNLDYIMNPVYAPLVFFIPVV
                     IGMGGNIGTQSSSVTVITLSNKDLNFSNVVREGIVGIITGLLCSIITGIVIYFVMRKL
                     DIVLIVSISLFINMVLGATIGAFMPVLLKKMDADPSTVSSPIISTALDITGIAVYFII
                     TTALLSKIV"
     gene            40973..41128
                     /locus_tag="ACGI59_000240"
     CDS             40973..41128
                     /locus_tag="ACGI59_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqzL family protein"
                     /protein_id="HFG9738054.1"
                     /translation="MNNISWEVFKKTGSIDAYLYFCDCKNIHEEIEEVREKKEDDDNP
                     EHPGDST"
     gene            41094..41867
                     /gene="recO"
                     /locus_tag="ACGI59_000241"
     CDS             41094..41867
                     /gene="recO"
                     /locus_tag="ACGI59_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFG9738055.1"
                     /translation="MIILNTQGIVLKAIRYKESDIILTLFTRKLGKVSAIAKGAKKNK
                     SSLLSSSQLFSYSNFTLKKQGNMYKVTQSEIIKSFYNISYDIEAFSYATYITKLVENS
                     ILENQTNNRLFILLAQTLYLYTQDNTDNRFITAAFELKFLDYIGFKPIVNKCINCECK
                     ILQNSVFNIYEGGILCSKCSKLFDNNIKLDLTTISLMEYVLRNDILTCSKAKVSKYIT
                     HELENILDKYLKVYVDNINFKSLHVLQSVKNNKGVDNDE"
     gene            41860..42459
                     /locus_tag="ACGI59_000242"
     CDS             41860..42459
                     /locus_tag="ACGI59_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4342 domain-containing protein"
                     /protein_id="HFG9738056.1"
                     /translation="MSSNITIEMVDSVFERVPGATYAEAKEALVMCNGDVIEAVIYLE
                     NKKNTCNCKAKSAKETMEEVFGKDGENIKFQLKELLRKSSVVRIIVEKDGKTMMNIPL
                     TVGVVGLAFVPLATLVGLSAAVISKYRIKVQNEEDGEIVDLGELNEEKLHVLKNMITN
                     AAKDVKDVVVDKKEDDKDVTADLMKEESEKNKEDEDENK"
     gene            42619..43497
                     /gene="glyQ"
                     /locus_tag="ACGI59_000243"
     CDS             42619..43497
                     /gene="glyQ"
                     /locus_tag="ACGI59_000243"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFG9738057.1"
                     /translation="MNFQEMILALQKYWSKQGCIMMQPYDIEKGAGTMNPNTFLRSLG
                     PEPWQVCYVEPSRRPADGRYGENPNRLYQHHQFQVILKPSPDNIQELYLESLKEIGID
                     PSEHDIRFVEDNWEAATVGAWGLGWEVWLDGMEITQFTYFQQVGNIECELETGEITYG
                     LERLAMYIQEVDSVYDLKWNDKITYGEVFNKAEYENSMYAFELCDADMLFNLFDIYEK
                     EALRLMENGLVIPSYDYVLKCSHAFNTLDARGAIGVSQRASFIGRVRNMAKTVAETFV
                     KQREEMGFPLLKDGDK"
     gene            43497..45563
                     /gene="glyS"
                     /locus_tag="ACGI59_000244"
     CDS             43497..45563
                     /gene="glyS"
                     /locus_tag="ACGI59_000244"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFG9738058.1"
                     /translation="MKNYLLFEIGVEEMPSRFVGSTLEQLKNNLSKLFDENRIAFDKI
                     NAYGTPRRLTLVVEGLSDRQNDLEEEIKGPAKKIAVDAENNLTKPALGFIKSKGLSES
                     DIYFKQVGKEEYIFATIKQDGKETSEVLKDILPETIKSVVFPKAMRWGGKNLKFARPI
                     RWIVALLNDKVLEFDLEGIISSNVTKGHRFLGESTIEVDSIEDYFEKLEKNYIVLDQN
                     KRKEMIRKQCIEVANSLGGEVEFDEDLLEEVTYLVEYPTAFYGEFDVDYIKLPKEVVI
                     TPMKQHQRYFPVLKEGKLLPNFIAVRNGDSYRIDNVKAGNEKVLEARLADALFFYRED
                     TKRNLESYIEKLKTVVFQVKLGTIYDKTLRLEKLSADILDSLGLVDEKADTIRAAKLS
                     KADLVTGMVGEFDELQGFMGKEYAKVGGENDVVSEAIFEHYLPRFAGDILPKTNAGVA
                     LSIADKLDSIAGFFAIGIQPTGSQDPYALRRQALGILSILMDRKVAVDIKVLIEHALD
                     NYSELDFNKEEVVEQIMNFFNERVKNLFKDLGIRYDVVDAVLSSDINDVSDMHMRALE
                     LNNWLEKDELVEMLTAFNRVSTLAQKAESDKIDESLLKEDAEIKLYNEFKQVKVKVNE
                     LLKDKKYGIALDEFASLRPVVDNLFDTVMVMDNDEAIKNNRLALLKQVYDTMLGICDL
                     SKIVYK"
     gene            complement(45729..47276)
                     /locus_tag="ACGI59_000245"
     CDS             complement(45729..47276)
                     /locus_tag="ACGI59_000245"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HFG9738059.1"
                     /translation="MDNFKKILLNELPNFKEYSSKFLNGEINKMQYKGFSGGYGVYAQ
                     RDKKSFMIRLRVSAGVLSQYQLNKIYEIAIKHNLDKIHLTTRQAIQLHDLSINSIVDI
                     MEEGIKNDIFTRGGGGNYPRNVGLSPLSGVDPSEVFDVTPYAVATDKYFIKNATTYHL
                     PRKLKVSYSNCYTDTAHCSIQDLGFVATLKNDEPYFRVFLGGGLGKQPKVALELDELI
                     KPKDALYCVEGMIKFFMDYGNYENKNRARVRYMVESLGEKLFLEKFKEYYKLEKENGS
                     LELNIEEIDYSKPGVKIDIQDSRLIPQKQDGLYTVYIHPVGGILLTKDLSTLLKELDN
                     IENPMIRLGMTEGLYILNLNGNEAKRILEISKTISCNSQLEQSISCIGVPICQMGIQN
                     SQKMLHEIIDYFRLQNNKEILNKAPKLYISGCLNSCGVHQVGSIGLCGKKKNVDGIST
                     DAFELFVGGSFEIGKTRLGKSLGDFKASDIPEMLYKISDASSGNFYEWVNSNDNILNK
                     ITDKYKI"
     gene            47556..48323
                     /locus_tag="ACGI59_000246"
     CDS             47556..48323
                     /locus_tag="ACGI59_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFG9738060.1"
                     /translation="MGEIINALDRALDIILLLYHEKREMGITEISKAMGVYKSTVHRT
                     LVTLENKGFVIQNAENGKYWLGINLYAIGMVVGEKMSLTEIVKPYTKKLNQEFNEVVN
                     VSILEERAQDSPRSIIIHKEYGSNQLLSVNPSVGSSSECYCSAVGKCLMAFNDSIDFE
                     KYRKTPIHKYTEHTIDNWDDMMLFLAKIKEQGYAIDDEELEHGLTCIGAPILDKNNKA
                     IAAISLSGPTIRMREGDFEYKIKRVIETAKSISELFR"
     gene            48606..48803
                     /locus_tag="ACGI59_000247"
     CDS             48606..48803
                     /locus_tag="ACGI59_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFG9738061.1"
                     /translation="MEKVKLSVERCKGCYLCIEACKKDAISLSDYTNKKGFVTIKVDE
                     EKCIQCGACYTMCPDLVFEIL"
     gene            48832..49908
                     /gene="vorB"
                     /locus_tag="ACGI59_000248"
     CDS             48832..49908
                     /gene="vorB"
                     /locus_tag="ACGI59_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HFG9738062.1"
                     /translation="MQSLLIKGNEALAEGAIRGGCRFYAGYPITPQSEILEMMAWRQK
                     EVGGVFIQGESEIASVNMVMGASALGARAMTSSSGPGFSLKQEGIAYWVSAGLPAVVA
                     CVQRYGIGDGFIGAGQDNYWQAVKGGGNGDYHLIVLAPNSVQENMDMAYESFELAEKY
                     MHPVLILSDATIGQMVEPCIAPPMKEYDMDKAPWAAKGTPVGEKNKVITDITYFEDQD
                     TWQKGYIEKMRKVQEDEQRWEEIEIEDAEVVFVAYGISSRIAEGAVRDARAKGMKLGL
                     IRPKTLWPYPRKAFKKLSDSLKGLVTVEMNMMAQMKEDVIIACDNRFPVYSVATAQYM
                     AEVNQLIDFAQQIIDGKAKQEEVF"
     gene            49911..50630
                     /locus_tag="ACGI59_000249"
     CDS             49911..50630
                     /locus_tag="ACGI59_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HFG9738063.1"
                     /translation="MAKKAPAIMMCENMFCGGCGHSIVDRIIGEVLTEMDICQDTIIC
                     SDIGCNHMFQFSMNVDVVVPPHGKMGAAMAAMKRVRPDKYIFTIAGDGGAYAIGLGET
                     MSAATRNDNVIFLVMNNNMFAMTGGQMAPTTIEGQYTASTPAGRNYADHGSTFDVVKV
                     MGNLDIAYLARGTITNPKEINTTKRYIKKALEKQKEKKGFCLIEVLVPCPTNWGLKPV
                     DAMKRIENELVPKYPLGEFID"
     gene            50643..51182
                     /locus_tag="ACGI59_000250"
     CDS             50643..51182
                     /locus_tag="ACGI59_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HFG9738064.1"
                     /translation="MLNEIICAGFGGQGVLTAGKIIMYVAYKAEKKITWFPSYGNEMR
                     GGSANCNVVVSDEKIANPCVKNPDILLGMNSVSVDKYESLMKKNGYMFVNESLVSKDR
                     KYREDINVIKVPVTEIAQNLGNEKAANICMLGAMVKSLDLFTKEEFESGMCEYFENQG
                     KGKFNSKNVEAFEAGYDYE"
     gene            51339..52397
                     /gene="buk"
                     /locus_tag="ACGI59_000251"
     CDS             51339..52397
                     /gene="buk"
                     /locus_tag="ACGI59_000251"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HFG9738065.1"
                     /translation="MKVLVINPGGTSTKISVFQDENEILKKNITHTREDLKNFSKVFD
                     EYDYRKQLIVDILSSENHSINSFDAVVGRGGLMKAIKGGTYTVSEEMIEDMRNEINGE
                     HASNLGALLAKTIADEIGVQSFVVDPVSVDEFDDVSRITGISDIEKASWLHALNHKAV
                     SRKIAQKLGGKYEDYNFIVAHLGSGISIVAHKQGKMIDGSGGRSDGPFSPERSGGLLA
                     YPLIELCYSGKYTHDEMVAKVSSIGGMYDYLGTKDMIEIENRIKEGDEKAKLIMDAFI
                     YQVAKEISMYGASLSGNVDRVILTGGISHSEMVVKGVAEKVSYLAPIEIVPGEMEMEA
                     LALGALRVLNGEEIAKTY"
     gene            52415..53308
                     /locus_tag="ACGI59_000252"
     CDS             52415..53308
                     /locus_tag="ACGI59_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HFG9738066.1"
                     /translation="MLKDFNNLLEEAKKLEPVIVSVAVAEDKEVLKAVKSATDLNIIK
                     PILVGDKDKIESISREIGLKGHEIIGCQNPDESVKKAVEIVKNKEAKVLMKGLVNTSV
                     YMRAILNKETGLRTGRLLSLLAVYELPQYHKLLYCTDSGINVSPNLNQKKDIMTNALL
                     TMKSIGMENPKTAILTANEMVDPKIQSTVDANLLVEMEKSGEIPKCIVEGPIAFDVAF
                     DAHAAEHKGIKSNIAGDVDLLVFPNIEAGNILGKSWLRFNKAKWAGIVLGATNPIILG
                     SRSDTAEIKINSIALACLASQ"
     gene            53338..54546
                     /locus_tag="ACGI59_000253"
     CDS             53338..54546
                     /locus_tag="ACGI59_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFG9738067.1"
                     /translation="MKYAKRMDMVKASAIRDSQKKIAQKVASGGTVISFAAGLPDPNL
                     FPIEEISEITQKVLNERGKFALAYGPTKGEDELLDLLVKRMKEEENIDTKAENIIITT
                     GSQQGIALSAMILLEKGDVVVTENPSYLGAINAFRPYECDFLGVDTDEEGMVTDDLDK
                     LLSENSNIKVIYVIPTFQNPTGKTWSLQRRKDFMEVVNKYDVIVIEDNPYGEIRFTPE
                     PLPTLKSLDTKDKVLYLGSFSKVLCPGFRVAWMCGNVDIIDKAELLKQGIDLQSNQFS
                     QVQVIEFLKKYNLNEHIEKIRAEYKKRCLLMLDAMKKHFPEEAKYTEPDGGMFIWVEL
                     PESINVDDLLDKAIDAGVAYVSGESFFANNGPKNTMRLNYTTMSEEQIVKGVEILAEV
                     IKKELNYACE"
     gene            54570..55829
                     /locus_tag="ACGI59_000254"
     CDS             54570..55829
                     /locus_tag="ACGI59_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFG9738068.1"
                     /translation="MGEKENGIKFGKLMFQLVIIAISWELVYIIPFIQYTLYDPILKA
                     LECTNTQLGFLLTIYGLGNIFGAPIGGWLADRFDYRKIILGSVFLNGVVSFLFAFNMN
                     YPFAVATWVGCAITSLVMNYPSMVKILRVIGKDNQGKVYGFNEAMVGVSGVVMGAIFL
                     YIYTCFATPTLGMRWVMISLGILSIAMCPVLWFVIKDVDIKEEREEAGEKMSAGDFIT
                     VLKSPNTWLVGISIFCVYSFTVTMSYFTPYITSVLGGSVALSGALAIIRQHGLKLFGA
                     PFGGYCADKLKSPTKVLLPIYVFGIAVIVLFLVLPASTPMTIFIALTFVVGILGYMGK
                     GIYYAVQDEVKVPVKYSATTIGIAAALGFSPDVFQFALIGHWIDTYGNKGYTYTFIFQ
                     IAILVIGILSCLYILKVKKRRASKLEA"
     gene            56474..57937
                     /locus_tag="ACGI59_000255"
     CDS             56474..57937
                     /locus_tag="ACGI59_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HFG9738069.1"
                     /translation="MTDLVNTINGIVWSPVLVAMCLCIGLYFSLKLKFFQIRDVKEMF
                     TLLLEGDSSHVGISSFQGFATSMAGRIGTGNIAGVAVAIAMGGPGALVWMCIMATLGS
                     ATAFVESTLAQIYKDEHDGQYRGGPPYYFEKGLGWKKCSVLFSIVAIISYTFFMPGTQ
                     SNTLYLAMNQAFGFSKMTIAVASSIALAIIIFGGIKRIGLFAEKVVPIMGGAYLIMTL
                     VIIITNISLVPEAIVTMFKSAFGFGSVFGGMLGSAISWGVKRGIYSNEAGEGSGTYGA
                     AAAEVSHPAKQGLVQAFSVYVDTLLICMATGIMIVITGMYNVTGVCTALPGVEPGPQY
                     VQSAINTLHPQLGDIFIAIVLLLFTFTTLLAFSYMMETNVSYLVTRIKAEKHEKYIIN
                     VCRFMLIACAAMCCFIEPLTSWALGDICIGVLTYINLIAIFTLKKPAIKAYYDYIRQK
                     KMGIKRSDRTFNPIELDIRNAEFWENRYLKEDKHELN"
     gene            57993..59129
                     /locus_tag="ACGI59_000256"
     CDS             57993..59129
                     /locus_tag="ACGI59_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFG9738070.1"
                     /translation="MKERIIQVADSKKEKILGLCQSLYDEPEIALQEYKSAKKISEFL
                     REEGFDVEENLAGMATAFKATKKNGDGPKIAFIAEYDALPGNGHACGHHLIASMGVGA
                     GIALSSILDTYKGEVSIIGTPAEETGDGKPYLIEHGVFDGYDAAMMIHPNSKTCVTPE
                     IIAIGGLDFIFTGKASHAGAKPYNGINALDAVVLLYNNINALRQQLVDGTRIHGIILE
                     AGTAANVIPDMGKVRLEIRAKEQNYFDEVVEKVKNCARGAAIATGCELEFYHFEPTCQ
                     GLNENKVLVDIFTKIMEEFGIYEDEQVMLGSTDMGNLSQIMPCIHPLMKFSENGEELH
                     TKEFLEASINSYAKDRVIDGIKILALTGFNLFENPELLKKMKEE"
     gene            59365..60480
                     /locus_tag="ACGI59_000257"
     CDS             59365..60480
                     /locus_tag="ACGI59_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family autoproteolytic
                     acyltransferase/hydrolase"
                     /protein_id="HFG9738071.1"
                     /translation="METLEVRNIELSGTNYEIGYRLGELVANMPEIIEGQINKSNLVS
                     KKEEKEMIELFDKYCPGLNEELQGFADAIQVNCNQILYYTMTYLKPGCSQVALAPELT
                     ENGHVLFARNFDFSHNMEDFVLCKTKVNGKYAHIGTTIMQFGRGEGMNECGLGVSQSS
                     CGIPVGNSDGLRKPAIVGLQFWAVIRYLLENCKDVDEALEYLKDMPIAYNINLLLADK
                     SGNIALVETLDGKKEVNIINSLESKREYFLHSTNHTHIDKLHKLDPQSMKNSIHRYKL
                     IKEYINKSKKIGEKELMNLLSSKYPNGLSCNYYNDFFGTLKSIVMDLNIGKFNILWGG
                     LENKWESYYLKNDIKSITQRININIEKAPSDFFDFIE"
     gene            60632..61501
                     /locus_tag="ACGI59_000258"
     CDS             60632..61501
                     /locus_tag="ACGI59_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HFG9738072.1"
                     /translation="MDYLKYFEKAVMYIENNLHENITVNDVAKETGYSYYHLTRLFKS
                     MFGESVGSYIKKRRLVSSTKELLYSDKKVIDIAIGSGFESSEAFSRAFKSIYKVSPIE
                     YRKNRIDVFVGKKKKLELDFMRHLVGNITIKPVIKEIEKIKVIGIKDKVILENNSLPD
                     LWEKFRKVHHIVPNTLPSRRVFGICEAISEIHLVSESMEFNEIIGLEVNSYDVIPNSF
                     VSKTIKEGKYAIFTHTGSLDNLDKTYEYIWGTWFLSSKEELDIRDDFEVYDERFLGPS
                     NVNSQIDIYIPIK"
BASE COUNT        24486 a         6480 c        11197 g        19392 t
ORIGIN      
        1 gaaaaattat aaaaatataa atttatatgc ttaattaaga taattttttt cattagtatg
       61 tatgatagat gcatcaaaca atataactaa tatgttagaa ggttatatgt aattaatgtc
      121 agtgtttata tagaatagca tttgtagcat ttattatagt tgtaattact atttatatca
      181 tagacttgaa tagtctaaaa aaatagacta taatattttt atagactaaa gaaagggctg
      241 ataagatgga catagcaaaa ctaattttaa atcctgcaag gcttcgtatt ttacaatata
      301 ttcgacttta tggcagtgtt cgtacatctg atattgtaaa gtatttaaat gatattcctc
      361 gtgcaacagt ttatcaccat gtaaaaattt tagaagagaa caatatgatt gaagtaatta
      421 aagaaaatcg tgtgcgtggt actattgaaa aggtttatgc actaaaggaa tatacaacat
      481 ctatggaagg agagaccttc gttgcacttt ctacagcatt ttatgtaggt ctgatgcagg
      541 aaatgaatga atactttagc agaaaagagc aagaccataa aaaggataat gtatttttct
      601 cttcagcatt actatatatg agtgataatg aatatgaaaa tcttcttaaa agtatagctg
      661 atttattgaa accatatata gaacaaaaac ctaaatcaaa tctaaaactt agaaaacttt
      721 caattatatc ttctccacct gtagctgata atgataaaac aaaataacat gcaacccatt
      781 tttataaaag gaggttatat atgaaatttc atgagtttgg aaataaaaac agaccacata
      841 ttatgttgat tcatggggga gggaatgcat ggtggaacta tttgcgacaa gcagaagttt
      901 tgtctaacaa atatcatgtg atacttccta cattggatgg tcatggagaa gaatatgaaa
      961 caacatacat ttctacagag gatacagcag ataagttgat atcatatata gatgaaaatt
     1021 gcagtggaca tcttttcgct ttgtgtgggg tatcacttgg tggtcaaatt gtcatggaac
     1081 ttttatctcg aaaacctaat attactaaaa aagcaattat tgatggtagt atttgctacc
     1141 caagacctct tatggcaagg ttttgtattg ctggtattcg attttttgga ggacttttgt
     1201 ttagtgagaa ggcttgtcgc tttcaaattg ctatgatgcc aaaattgtta cctgaaaata
     1261 tgcaatatcc agataaaata aaagcgtatt atatgacaga tatgccacat gtaagaaaag
     1321 aaacattcta taatatgtat cgtacttaca tgatgaacta tacacttaaa gagaatgtta
     1381 aaaaaaccac agcacaagta atgtactggt atggagaaaa agaaatgaag tgtgtcaaaa
     1441 aatctgcaaa actgtttcag tcttatgtgc cctcttgtga gatttatgaa gctaagagct
     1501 ataatcatgg gtatttatca ctttatcttc caaatgaatg gttggaaatt gcagaaccat
     1561 ttttccaaaa ggaataaagg tgttaggtga ttattgtata attattgctg tttttttata
     1621 tagtaaataa attaggtata aatataaaaa attattttag ttaatatatt agtgaaagct
     1681 taataggaaa attataaata gattaaaggt agcacctata taaatagttg ctaccttttt
     1741 ctgtttgtaa ataaatgata gataagaagg tttactgaca aatcactgac taaacccaat
     1801 tttcttacat tgaaagcctt aattttactt gttttaaata aatttacaat ttcttgttat
     1861 aaaatactaa attttgttaa caattataat tagcatttaa tttatactgg aggtttatat
     1921 gattagtgta agaacagatt tagctttaga agctagtgaa atgtgtgaaa agtctcaaga
     1981 aggaagtagt attcctggtg taaaaataga aactaaagaa ttagaaaatt gcattgtaac
     2041 taaagttgaa gtaatagaca ggcaaggcgc agagataatg aataaagata taggaaagta
     2101 tataactctg gaaagtaatc ttatgaagtt tgatgatgat gagtcaagag aagaaatgat
     2161 aagttattta aaagaggagt tagtagatat atttggccaa gacaaaaata aaaaaaccct
     2221 ggtaattggt cttggaaata gaaatattac ttctgatgcg ttaggaccaa agagtgtatc
     2281 aaaaacactt gtaactagac atctttttaa aaattataat aaagactatg atgatgattt
     2341 cactgaggta tcagctttaa gcccaggagt tatgggtgtt actggaatag aaactagtga
     2401 aatagtaaaa tcattggttg agaaggttaa gccagataga gtagtagcaa tagatgcatt
     2461 ggcttcaaga aaaatggaaa gagtaaactc tactatacag atatcaacag caggaatatc
     2521 acctggtgga ggtgttggca atactagaaa atctctaact aaagaaactt taggcgtgga
     2581 tgttattgca ataggcgttc caacggtggt tgatgcagca actcttacaa tagatgtctt
     2641 agatatggca atagacaatt taatagctca atcagaagaa acagaaagtt tttatgaaat
     2701 gttgaaaaaa cttaaagaag aagaaaaata tcatctaata aaagattcgt tagacccata
     2761 tgacaagaac ttaatagtaa caccaaagga tatagatgat acaatcgaaa atctatctat
     2821 aataataagt gaaggcttaa ataggtcttt acatccaggt agattggtat aaaataagga
     2881 ggagcaatta tgtttaaaaa atgcattaaa gtagtaacct tgacttttat attagtatgc
     2941 attttaccag gaaaatcatt agctttgaat caagatgatt ttttaaagtt tttagtaaat
     3001 tcatcttatc cagaagctaa agtcgaagga aatgatacag aaaataaaaa gaataataaa
     3061 aataaagaaa caagtaagga aaataaggaa gaaagtaaag aaggaaacac taaatctaaa
     3121 gatgcaagta aagttgataa taaaaaagag agtgaaaaag aatatataaa actttatgtt
     3181 ggtaaagaaa atgttccaga tattgagtca aaaaattcag acacaactga aactaacaca
     3241 acatctagtt cagattataa ggatgattta agagtaacca aagaaaatcc aagaattttg
     3301 atatatcata ctcatggatg tgagacttat tcaaattcac ctgatggaaa ttatcattca
     3361 agagataaga aaaattcagt aatggaagta ggaagtgcat taactagtgc tttagatagc
     3421 aaaggatggg gtgtagtcca tactactaaa taccatgact atccatctta taataactct
     3481 tatgcaagta gcttaaaaac aatacaaagt atattaccta aatataactc agtggatata
     3541 gcgatagact tacatagaga tgctagggat ttaactaatc cagccactaa ggaaaaagac
     3601 catttaaaat atacaactat gataaatgga gagagagtat caaagttctt ctttgttgta
     3661 ggaggtaaaa atacaaatag aaaacaactt agagctttag cagaagatat aactgctttt
     3721 gctgagaaaa agtatcctgg tctagtatct ccaatagtag aaaaagacta tgctagattc
     3781 aatcagtttg cagttaagaa tcatatgtta gtagaaatag ggaataacgc aacaagtgta
     3841 gaagaatcta aggctactac aaagtattta gccgagattt tggatgaata ttttaaacaa
     3901 aaaaattaga gctttaagtt ataattagga gcatgctaaa tgagccgatt agaaaaaaat
     3961 atagaaagta aaagtaaact aagaaaatac aagtatatta tgagaatctt atttttgatt
     4021 actatgatta tatgtacatc agtttgtgta tttatagtgg acagtaatgc aaaaagtatg
     4081 ctaggaatga aacaatatga ttttccaagt attaaatttg atataacaaa atttgatttt
     4141 gacataccaa attttgatat acatgaaatc ataaaaaaat ttaataagta gtaataagtg
     4201 aataaggttt attgattaaa ataagcctta ttttagttat atatttttaa tgtataaaat
     4261 aaaaattcat taactgtatt ttataggatt tactttaagt ttatgatatt atataatatt
     4321 tagtgttaga cttgataatc cacatataat gtggataatt ttgtttagtg ccaattagtt
     4381 tatattcaaa aattttaaat ttggtatata aagagttttt agataatgta aaataataac
     4441 tataagaata atcaagagga gggaatttta aggtggacaa taaacaaagt agaactagaa
     4501 attttagtat aattgcacat atagaccatg gaaagtctac cttagctgat agattaatac
     4561 aacaaactgg gcttgttagt gaaagagaca tgaaaagtca attacttgac aatatggacc
     4621 ttgaaagaga aagaggaatc actataaagc ttcaaaacat aagattaatg tataaagcga
     4681 aggatggaaa tgaatattat ttaaatctta tagatacacc tggacatgta gactttaact
     4741 atgaggtatc aagaagttta gctgcatgtg aaggagcact attagtagtt gatgcagctc
     4801 aaggtgttga ggcacaaact ttagccaatg tttatcttgc tatagaccaa gatttagaaa
     4861 tattaccaat aataaataaa atagatttac caagtgcaag accagaggaa gtaaaaaatg
     4921 aaatagaaga tttaataggg cttgattcaa gtgaagctcc tcttatttca gctaaaactg
     4981 gtttaaacat agaagatgtt ttagaagata ttgtaaaaaa tgttcctcca cctaaaggag
     5041 ataatgaagc tccattaaag gcattaatat tcgattctta ctatgatgct tacaaaggtg
     5101 ttgtagctta tgttagagta tttgaaggaa ctgttaaaaa gggtatgaca ataaaaatga
     5161 tgaataccaa taaaaagttt gaggtaactg aagttggtgt tatggctcca ggtcaaactg
     5221 aacttagtga attatcagca ggtgatgttg gttatattgc tgctagtata aaagatataa
     5281 gaagttgtcg tgttggtgat acaataacag attcaaataa tcctacagaa gaacctttac
     5341 caggatacaa aaaagctact ccaatggtat attgtggtat atatccagga gaaggagaaa
     5401 agtatgagaa tgttagagat gctcttgaaa agcttcaagt aaatgatgct gcacttgaat
     5461 atgaagctga gacttcagca gcactaggat ttggttttag atgtggtttc ttaggtcttt
     5521 tacatatgga gataatgcaa gaaagacttg agagagaatt taaccttgat ataattacta
     5581 cagctccatc tgttatatat agagttacaa aaatggatgg agaggttgta atgatacaaa
     5641 atcctgcaaa cttaccagaa ccatctgaga taaagatgat agaagagcct atagtaaaag
     5701 gagatataat agttcctaaa gactatgtgg gtgttgttat ggagctttgc caggaaagac
     5761 gtggaaatat gttaaatatg gaatatatag atgatagaag agtaatgctt cactatgacc
     5821 ttcctctcaa tgaagttgtt tacgatttct ttgatgcttt aaaatcaaga actagaggtt
     5881 atggttctct tgattatgag gttaaaggtt atgtagcatc tacacttgta aaacttgata
     5941 tattaatcaa taaagagcaa gtggatgcac ttagttttat tgtccatgag acacgtgctt
     6001 tccctagagg aaaggcaatg tgtgagaaat taaaaggaga aattccaaga catcagtttg
     6061 ctataccaat acaagctgct gttggtaata aggttattgc aagagaaaca ataagcgctc
     6121 taagaaaaga tgttcttgct aaatgttatg gtggagatat atctcgtaag aagaaacttc
     6181 ttgaaaaaca aaaagaaggt aagaagcgta tgagacaaat tggttctgtt gaagttcctc
     6241 aaaaagcatt tatgtctgtg cttaaattag atgagtaaat ccttataaat ttagataaag
     6301 atatttaaaa gctagagtga ttttagaatt atttctaaat tagtctagct tttttatggt
     6361 atattttaag taaactcata gagaatataa gtaagaatat taaagaagaa taaaaaggtg
     6421 atatttatga ttaatataga aattcctaaa aaagtagact atataattaa agaattagaa
     6481 aagaatggtt atgaagctta tatagttgga ggatgtgtta gagattgttt attagaaaga
     6541 gttccaaacg attgggatat aacaactagt gctagaccag aagtggtagt agaattattt
     6601 gaaaaaacaa ttccaactgg tatacaacat gggactgtaa cagttatgat tgaacatgaa
     6661 ccatttgaag tgacgactta tagaattgat ggaaattata gtgatggtag acatccagat
     6721 tcaatagaat ttacaaataa catagttaag gacttgtcaa gaagagattt tactatcaat
     6781 tcaattgcat acaattcaaa gacaggatta gtcgacccat ttaatggcta tgaagatata
     6841 caaaataagt atatacgttg tgttggaaat ccagtagata gatttgaaga agatgcactt
     6901 agaatgctta gagcagttag attttcagca caactcaatt ttaaaatagc tgaaggtact
     6961 aagcaaagta ttcataaaaa agcagatttg ataaaaaatg tttctattga aagaatccag
     7021 acagagttta ataaaatatt agtatctgat tcatctaaat taaacttatt aaaatcaaca
     7081 ggtttgttga aatttataat tcctgaaata tgtgaattag aagatgttac tcaacacaat
     7141 ccatatcaca tctatgatgt acaaaaacat actttaatag ctacagaagt aattgaagat
     7201 gaactgtatt taaaacttac aatgttgttt catgatttag gtaaaaaagt aactaaaaca
     7261 actgataaaa atggtgttga ccatttttat actcatagta gagaatctgt aaagattgca
     7321 aagaagattt tgaaaagact taagtatgat aattatacta ttaataaagt tttaatatta
     7381 atacaatatc atgattatag aatagaacct aaaaggaaga taattaaaaa gttattaaat
     7441 aaacttgaag atgttgaatt atttgaagat ttaataaaag ttaattgggc tgatacactt
     7501 gcaaaaaatc caaagtatgc aaaacaaaag atattaaatc ttattgagtg tgaaaaagaa
     7561 tttaaacata taataaatca aaaggagtgc tttaatctta aagatttagc tattaatgga
     7621 aaagatttga tatctatagg tataaaacca ggtaaagata taggatatat tttaaataag
     7681 atgcttgaaa tagtcataaa taatccagag ttaaatgaaa aagaaatact aaaagaaaaa
     7741 gcattaaata tatatacatt ttagattcaa atataaaaaa tttaattact ttaaaaaaca
     7801 tagaataaaa tacttattta gataaaataa ataattttta gagaatattt tagaagttac
     7861 gattgtatga tttttaaatg cataggcatt tactctagga ctatagacta atataaaaaa
     7921 aaactttggg aatactaaaa ccataaattt ataaaattaa tattttatat actgtaaaac
     7981 agtgaggagg gacagtatgc atactcaact acaaaagact attggtcttt cagcagctct
     8041 ttctactgtt gtaggtatgg ttataggttc gggagttttt tttaagccac aagcaatata
     8101 cactacaact aatggagctc caggtctagg tattatagcg tggcttttag gtggttttat
     8161 aacaataact gctgggctta cagctacaga aatttcagct gctataccaa aaactggtgg
     8221 tatgatgatt tatatcgaag aaatatatgg agaaaagtta ggttttttaa ctggatggat
     8281 gcaaactgta ttatttttcc caggtacatc agcggcatta ggagtaatat ttgctcaaca
     8341 agcatctgaa cttttaggaa tgagcccaaa taatatggca aatgtacttc ctatagcaat
     8401 tggagttata ctatttttag cgttattaaa tataataggt tcatccttag gaggaaaagt
     8461 tcagacagta gccactattg gaaagatgat acctttaatt ttaataatag tctttggatt
     8521 tattaaaggt caaagtagtg aagtattaaa tccatttgta ggagatggtg taaatgcatc
     8581 aaatgcactt ggacaagtat taatagctac tttgtttgca tatgatggtt ggataaatgt
     8641 tggagctata tcaggagaaa tgaagtctcc agagaaagat ttaccaagag caatagttgg
     8701 tggtttatca ttagttatgg cagtttatat tataataaat atagcttatc tttgggtagt
     8761 accagcaagt gaattagcaa cagttacttc cccagctaca ttagtagcaa caagattgtt
     8821 tggaaatata ggtggaaaag taataactgt gggtatatta atatcagtat ttgggacatt
     8881 gaatggatat ttattaacag gttcaaggat accttataca ttagctgaaa tgggaacttt
     8941 accagcatct aaaactcttt taaaagtaaa ttctggaggt tctcctgtaa attcaatact
     9001 acttataact gtgttagcct gtgtatatgc attatctggt caatttaatc ttttgacaga
     9061 cttaacaata ttttcaatat gggtatttta tgtattgact tttataggtg ttatgaggct
     9121 tagaagacaa aagccagatt tacacagacc atataaagtc cctttatatc caataatacc
     9181 aattatagct atattaggtg gactgtttgt aataataaat caaatattga cttcaacagt
     9241 catatcttta ggcggaatat ttataacact attaggttta ccagtttatt attatatgaa
     9301 aaaaagataa ctacttaatt taagttagat aaatttgggt aaaggtgaaa attaactttt
     9361 atccaaattt tttatactgt tatacaatat aagtattata tattgcttga tgtgctattt
     9421 tgtttgattt ataatttagt aattgatgtt ttaagataat ttaagaaatg aggagtgaaa
     9481 gatgctagga ctttatgtac atattccatt ttgtgtgaaa aaatgtaagt attgtgattt
     9541 taattcttat aaaatggaca tagattcaaa aaaaagatat atagaagact taaaaataga
     9601 aatggaatta tatagtaata aattatataa agataataaa tataaaaata aagaatgctg
     9661 tagtctaaat aaaaatgaca aaataacgag tatatttgta gggggaggta cacctagtat
     9721 actaacctct gatgaaataa gagaagtatt tataagtata aaagaaatgt ttgatataga
     9781 tgagaatgca gaaataacta tagagtgtaa tcctgggaca ttaacattag aaaaattaaa
     9841 aactatgaaa gagattggca taaatagact tagtataggg ctacaagcta ttcaagaaaa
     9901 gcatttgaac tttataggaa gaatacatac ttatgaagaa tttgagaaaa actataaaga
     9961 tgcattaagt gtaggtttta aaaatattaa tatagattta atgtacagtc ttccaaatca
    10021 aactctatgt gattggaagg aaactcttga aaaagttgta cacttaaatc caacacatat
    10081 atcagcttat tcccttatac tagaagaagg aactgaacta tacaatatgt atgaaagtaa
    10141 taaatttgaa cttatagatg aaaatgtaga tatagaaatg tatgagtata caataaacta
    10201 tttaaagtca aaaggatata atcaatatga aatatcaaac tactcaaaag aaggctataa
    10261 ttgtgagcat aatattttat attgggaatg tgaacattat atagggattg gagcaggagc
    10321 ttctggctat attaatgaga acagatacaa taatgtagaa tctttagaag attatcattt
    10381 aagtttggta aaaagagaaa aaccaataca agaaaatgaa attctttctg agaaagatat
    10441 gatagaagaa aagatattta tggggcttag aatgaataaa ggcataaaat ttgaagattt
    10501 taaaaaaaaa tttggaatag attttagaga gaagtataat aagcaaatag aaatgctatt
    10561 agctagaaag cttataaatc agagctttga aggtatacaa ttaactcaaa aaggaagaga
    10621 aatatctaat agtgtattta tagaatttat ggaataattt ttaaggtgtt gacaaaataa
    10681 atttataatg gtatataata tatataatct ttttagcact cgaacttagt gagtgctaac
    10741 agctaggagg tataataata tggaattaaa tgaaagaaaa ttaaatatcc tcaaagctat
    10801 agttaaagat tatatagaaa cagctgaagc tataggttct agaacaatat ctaaaagaca
    10861 tgatttaggt gttagtgctg ctaccataag aaatgaaatg gctgaccttg aagaattagg
    10921 gtatttaatt cagcctcata cttctgcggg tagagttcca tctgaaaaag gctataagtt
    10981 atatgtaaac tctcttatga gtaaaagcga attggatgat aatgataaga ttcttattga
    11041 acaatgtatg aatcataata taaatcatat aaaagaattg attcatgaaa cttctaagct
    11101 attatcgcaa ctaacaaact atacaactgt agcagttact aagagtttaa taaatcaaag
    11161 tgttataaag cacatacagt tggttgctat gaatgacaat aatattttac ttatagttgt
    11221 aacagataaa ggggacttaa aaaaagctaa ccttacaact aatgtatatt tagatcaatc
    11281 taagttaaat ttgatttctg ataatcttac aaggaagctc ttaggtaaaa gtataacaga
    11341 tttggatgat aatctaattg cttttattaa atacgaaata agtgagtatt ctgggcttat
    11401 agatgagctt ttaaatgctt tgaactcaaa tatgaaagaa gaagattttt ctctatcatt
    11461 aaatggagct actaacatat ttagttatcc agaatttaat gatgtattaa aagcaaagtc
    11521 atttttaaat atgttggaaa aaaaggaaac aatagctgac ataattaaat caaagggaat
    11581 acaaaaagac aatctaaata taataattgg tagtgataat gattgtgaac tggcacagga
    11641 ttgtagtata gttacagcaa cttataatgt agatagagac ttggttgggc gaataagctt
    11701 tataggccct accagaatgg attatgctag aatttactct ataataaatt atatgagttt
    11761 attaattaat agaaagtaaa gaaattttaa attgcgaggt gtaaagtctt ggataaaaaa
    11821 aatgagcaac aggaagtaag agaagaaaat gatacttcta ttaaccaaga atcggaaact
    11881 caagtagagt tagaagagga agttgttaat gaagaatgtg aaacatcaag tgaaaaaact
    11941 gatgaaaaag aagttgatga tgaaaatgta acagatataa attctaagtt agcagaaaaa
    12001 aaattgcagg atgagttgga tgaattaaat gataaatatc aaagacttca agccgaatat
    12061 gcaaactaca gaagaagaac tcaacaagag aaagagacaa taggcgtatt tgcaaatgaa
    12121 aaaataataa ctgaattgat accagttata gatagtatgg aaagagcctt agatgcatgt
    12181 gaagataagg aagatactat gtataagggt ataagccttg tacataaaca attaatagac
    12241 actttagtaa aatttggagt agaagaaata gaagcagaat ctaaggaatt tgaccctaat
    12301 ctacatctag cagttatgca agaaagtgta gatggagttg aagcaaatca aatagtaatg
    12361 gtacttcaaa aaggatataa gctaggaact aaagttgtaa gaccttcaat ggttaaagtt
    12421 tcttgctaag tataaattaa aggttaaatt atttaaccta atataaaata ttaaaataag
    12481 taaaacttaa ttatataatt actcgaggag gattttagtc atgggaaaaa taataggaat
    12541 agatttagga acaacaaatt catgtgttgc agtgttagaa ggtggagaag cacaaataat
    12601 agcaaatagt gaaggtatga gaactactcc atcagtagta gcatttacaa aagatggtga
    12661 aagaatagtt ggagaacctg caaaaagaca agcagttaca aatgcagata aaacaataac
    12721 ttctataaaa actcatatgg gaactgatta taaagtaaat atagatggaa aatcatatac
    12781 tccacaagaa atatcagcaa taatattaca aaaattaaaa tcagatgcag aaagttactt
    12841 aggacaaaca gtaacagaag cagttataac agtaccagct tactttacag atgctcaaag
    12901 acaagcaact aaagatgctg gtagaatagc agggcttgat gttaagagaa taataaatga
    12961 gccaacagca gcagctcttg cttatggtat ggataaatta gaccaagaaa agaaaatatt
    13021 agtatttgac ttaggtggag gaacttttga cgtatctata cttgaaatag gagatggaac
    13081 ttttgaagtt ttagctacag ctggtaacaa tagattaggt ggagatgact ttgaccaaat
    13141 agtaatagat tatttagcag aggaatttaa aaaagctgaa ggtgtagatt taagaaatga
    13201 taaaatggct cttcaaagat taaaagaagc agcagaaaag gctaagaaag aattatcatc
    13261 aacaatgagt tcaaacataa acttaccttt cataactgct acagcagaag gaccaaaaca
    13321 cttaaatata gatttatcaa gagctaaatt tgaagaatta actagaggtt tagttgaaaa
    13381 aactatggaa ccaactaaga cagctttaca agatgcagga ctttctacag gtgatataga
    13441 tgatgtatta ttagtaggtg gttctacaag aataccagca gtacaagaag ctgttaagaa
    13501 gtttatagga aaagaacctc ataaaggtat aaacccagat gagtgtgttg cagcaggtgc
    13561 ttctatacaa gctggagttt tagcagggga tgttaaagac ttattattac tagatgttac
    13621 tccattatct cttggtatag aaactatggg taatgtaatg actaagataa tagaaagaaa
    13681 tactacaata ccaactaaga agtctcaaat attctcaact gctgctgata accaaacagc
    13741 tgttgatata catgttcttc aaggtgaaag aagtatggct tatgacaata caactttagg
    13801 aagattccaa ttaacagata taccaccagc acaaagagga atacctcaaa tagaagttac
    13861 ttttgatata gatgcaaatg gtatagttaa tgtttcagct aaagatttag gtacaggaaa
    13921 agagcaaaaa ataactataa catcaaacac taatttatca gaagcagaaa tagagcaaaa
    13981 gataaaagaa gcagaaatga atgcagaagc tgacaaacaa aagaaagaaa aaatagaagc
    14041 attcaatcaa gctgaatcta ctatatatca aactgaaaaa actttaaatg aacttggaga
    14101 taaaataagt tcaggtgaaa aagaggatat agaaaaagct atagcagact taaaagctgt
    14161 aaaagataat caagatgcta cagcagaaga acttaagaaa gctacagatg aagtaatgac
    14221 taagttccaa aaagtatctc aagaaatgta ccaaaaagca gctcaagaac aacaagcggc
    14281 acaaggtgca gaacaagcac aagacaatgg acctaaagat gacaatgtag tagatgctga
    14341 ctttaaagaa gtagatgaag ataaataagc tatataatat gtaacactaa atgataattg
    14401 gaacatatat gatttgtgaa aatcatatag atgttaaaga tatatggaca atatacatca
    14461 taaagaatat aatatatcat agattatata acttaaagta gaatataaga attaatgata
    14521 gcttgtagtt tattattcta caagctatcg ttttgtaaaa tgaaggtggt gtcaattttt
    14581 gagtacaaaa agggattatt atgaagtctt aggtatcagt aaaggtgcag aagctcaaga
    14641 gataaaaaaa gcttatagaa aattagcaat gaagtaccat ccagatagaa atcctggaga
    14701 taaagaggcg gaagaaaaat ttaaagagat aaatgaagct tatgaagttc tatctgatga
    14761 tacaaagaga aaaacttatg accagtttgg acatgatggg ctaaatggtc aaggcggttt
    14821 tggtggtcaa ggaggatttg gcggtcaagg ctttggtggt tttgaagata tgttcggaga
    14881 tatatttggt gacatgtttg gaggaagctt cggaggtgga agagcaagaa gaagagggcc
    14941 tcaacgtggt gcagatataa gacagagtgt tactatatct tttgaagaag ctgcatttgg
    15001 taaaaagatg tctataaaag taaatagaag tgaagaatgt gaagaatgta atggaactgg
    15061 agcaaaacca ggaacatcaa agaaaacttg ttcaacttgt aatggaactg gtcaagttag
    15121 aactgttcaa agaactccat ttggtaatat agcaagttca agaccatgta gtgcttgtaa
    15181 tggaactgga gaagtgattg aatctccatg tagtaaatgt catggaactg gaaatacaag
    15241 aaaagttaag actatagaag tagatatacc tgctggtata gatgatggtc aaatgataaa
    15301 actttctggt caaggtgaag taggagaaaa aggtgctcca agaggagact tatatattgt
    15361 agttaatgtt aagtcacatc cactatttac tagagatgga aatgatattt attttgaaat
    15421 gccaattaca tttgttcaag caactttagg tgatgagata gaagtaccta ctttggatgg
    15481 aaaagttaaa tatagtgtgc cagaggggac acaaactgga actgtattta gacttaaaga
    15541 gaaaggaatc cctagaatta gaggtaattc aagaggagac caatatgtta aggttgtagt
    15601 tgaaattcct aaaaaattaa acgataagca aaaagaacta ttgagagaat ttgcgaagga
    15661 gtgtgggtcg aacgtccacg aaaagaagaa aaccttcgga caaaagatag aagatatgtt
    15721 taagaaaaag tagttaaagt agccaatata taatcatatt tttaaaatct tagttattta
    15781 taagattgtc aaaggagata ttatgatttt tatattggct atttttgtat aaaaaataaa
    15841 tgtgcataaa ataaaactga atataaataa ttttctgaaa aatacttgtc ctaaaatata
    15901 taatatgcta taatataatc aaataaaaga aaggggtgat tccagtggga acggatgtgg
    15961 cactgccaaa tgatgaagaa aactttattg taatatacaa aataaaatta gatactttta
    16021 gtgctagatt aagtactgga aaaactcatg agctgggata attattgtaa ttacctagac
    16081 tcaaactttt agtagtaaaa tagtaatatt atatagacaa ggtatctaat ttttaaatct
    16141 tttgtatgaa ttaaaatttt tataaagggt atatctgatt agaatgtaat tagggaaacc
    16201 tagttgtgaa ttacttttag tatatacatg tgtaaagaaa tatgtatatg gtattggtta
    16261 ttgtgctaga tgtgtatgta aaggaatatg tgtctagttg ggtatatgta aagaaatata
    16321 cttataatct atgaaaaatt tttagtataa attgctaaaa cattgagatt gttttttata
    16381 gaaagttagt ttataccaac taaaaatgtg cttaagattt gtttgatgtt taattataaa
    16441 taaggttgta gataaaaaaa taagtaaaga caaaggttta aaatacttgc tttaaatata
    16501 gtttgttttg aacacttgcc aaattaaaat aattaataaa atagacatct tctcatagta
    16561 aaatatactg tgagaggatg tttttgcttt gttcataaac ttttatgaaa aatttgcata
    16621 gtgtcacatt ttaatatact atatatataa taaaatatag ataaaattaa atgattcaaa
    16681 agagaaaggt gaaaagagat gtattatata ggtgtagaca taggaggaac aggtatacaa
    16741 gcaggagtag ttgataacta tgggaaaata atttttaggt cagagtgtaa aacagtaatt
    16801 gaaaaaggct ttgaaggaat acttaatgac ataaaaataa tgatttataa gttgttggaa
    16861 gataataaac ttactatgag tgacataaaa tctattggat ttggtgttcc aggttttata
    16921 aataaagaag gattagtaac ttgtgtaaat ttaaaatgga ataaaaaagc ttttaataaa
    16981 gagttaaaaa gaagatttcc agatgttgaa attcatggag aaaatgatgc aacagtagca
    17041 gcacttggag aagcaaagtt tggaagtatg aaaggtgcta atgttggagt attatatact
    17101 ttaggaactg gagtaggtgg aggaatagta attaatcaaa aagtcttttc tggagcacat
    17161 ggattgggtt cagaaatagg tcatcaaatt ataggagaaa actattttaa ttgtaattgt
    17221 ggaaataatg gttgtgtaga aactttttgt tcagcaactg caattataaa gtattctcaa
    17281 aaactgatag aagaaggaga aaagagtagg attttagatt tggcagaggg aaatttagaa
    17341 aatgtaaatg caaagatggt gtttgatgca tatagagaaa atgatttagt ggcaattaaa
    17401 gtaataaata gatttaaaga gtatttagca aaaacttttg caaacacaat aaattcttta
    17461 gacccagaaa ttatatctat aggaggaggc atttctaaat ctagcgatat aatacttgat
    17521 ggaatagaag atttggtaag aaaatttgta ttatacaaaa cagaagatat agctacaatt
    17581 acatgtgcaa ctttaggttc agacgcagga attataggag cagctttttt ataatgtaaa
    17641 tgaactgctt attggaggcg tgatgatata tgggtaaaac aaatgctaga agaacgcttt
    17701 ttttgatagc aataggttca ggaactatgc tgaatcctct taattcttct atgatttcac
    17761 ttgctttaca tagtatacaa aatgattttc atattagttt tacaactgta tcttggattg
    17821 tttcagcatt ttacctagct agtgctattg cacaaccagt ttcaggtaag ataggagatt
    17881 taataggtag aaaggtctta tttttgagtg gtttgatgct tgtaatttta tcagcatcca
    17941 tagtaccact tgttcaatca ttctttatat tgatttttat aagagtaatt caagcaattg
    18001 gaacaagtac cctatatcca tctggagttg ctcttataca aaataatata aaagaaagac
    18061 aatcttctgc attagcagtt ctaaccattt ttgcttcaac tacagcagga ttaggtccga
    18121 cacttggagg attattgttg gacttaggag gatggcatgc tatatttctt gtaaatatac
    18181 ctgttgtttt ggtttctata tgcttagggt attttttatt cccaaaagaa gtaaaagaaa
    18241 agaaatctat aaaagaaact ttaaaaaatg ttttgtcaag attagatatt attggcatac
    18301 ttctattttc aattgcaatg atttttatat tactattttt gctatctatg aaggagtctt
    18361 ttaacttgga acaacttgta tttggtataa tactgatgtg tctttttatt tggcatgaat
    18421 tgagaacaaa attgccattt atagatatta agctttttgt aagtaatcct aaattgtcaa
    18481 aagtttactt gcaatttatt atattaaatt tctttaatta tattttgttt tttggtcttc
    18541 ctagttattt tcaagatgcc ttacattata gtgcaaagtc gactggatta tttatgctat
    18601 ttatgtctgg tataggtata tttatatctc cattaactgg aaaatggata gataaatctg
    18661 gaactagatt tcctcttgtt acatcatcaa tctttatgtt tattagtgca gtacttcttt
    18721 cagtagtttt tatccatata cctgtaatag gaaagggtat tatattatca ttagcaggta
    18781 ttagttatgg agttggtaat gtagccctgc aatcctctat gtttgaagaa agtcctcgtg
    18841 acagtatagg gacagcttct ggtctgtttc aaactagtcg ttatttaggt tctattttag
    18901 caactgtttt acttggaatg gtatttgaag taaatataac atcagaacaa tttcagatat
    18961 taggttatgt tatggtggtc ttaggtacaa taagtttttt attaaatttt atattaaaga
    19021 aagaattaag aaaagtagaa tagtggattt atggatttaa taagtctaat aactgataag
    19081 tttaaaaata ttgtttgtaa aacataactg gtttgatatt tattaattct aagttttgaa
    19141 gcatagagta ctcgataagt atacaatatt ttgaattaaa attaagcaga aatttgaact
    19201 agtctttcaa atctctgctt aatttagtag ttaaattttt ttatagagaa aataagattt
    19261 gacacttaga taaaaaatca ttgcattttc aaaattcttt aaatcaaacc caagtatctc
    19321 attaatcttt tccaatctat atactagcgt gtttctatgt aaaaacatac tagtagatgt
    19381 cttagaaata tttaaatcat tatttataaa ttttattcct gtatatagaa gttcttcgtc
    19441 aatagattta atcttattaa aatctattaa tttattgagt atattatcct cattaattaa
    19501 atctaaagtt ttatatctaa taaagtcaaa caaatttaaa aacttatttt cagcgttgtc
    19561 ttttataata tgaggtagta aatcagaaca taactgtaat ctaaaatcaa aatcagcttt
    19621 actatctaat tcaccaagat aaaaaatata agtactttga taagtttcag tttcaattat
    19681 attttgtgta gttacattaa tatctaagtt atatttagaa tttactataa aaaaaccatt
    19741 gtgttgtatt atatttatat cttcaaataa attataaaaa atatttttta gatattcaaa
    19801 gtctatatga gattggtaaa ttaaataaaa gttgcctttt aaattatcga gattattttt
    19861 atctagaaaa tcaataacat aaatagcatt attgaaagta actaaatggc tactagcatc
    19921 taacttttgg ttttcatatg gatagatatt tattttttta ttaagctgtc tttctaaaaa
    19981 atcttttaac tcttccataa agtctcctta aaaataagat tatattctta tcttatagct
    20041 aaagtagttt ctttgtcaaa tatgtgtact ctactattgt caactgaaag tgttaagtca
    20101 tcaccaacat taattctgtt tttactgtca aatctaattg ttatcgtatt ttcgcctagt
    20161 tttaagtgag cataagtttc tgctcccata agttctttta tttcaattct tgatttaaaa
    20221 caagtatctg gattagattt aataaaagta tcttctacat gaatatcttc tggtctgata
    20281 cctataacaa cttcattatt aatataattg tcttttatta aatcatagtg tttttcttta
    20341 ttcaatttaa gcttaatatg ttcattttgt gcataaattt catcattttc ttctattaat
    20401 attacatcaa taaaattcat ctgtggtgct ccaataaatc ctgcaacaaa tatattagct
    20461 ggataatcgt atacatcttg tggagttgca atctgctgaa ctactccatc cttcattact
    20521 acgattctat cagccatagt catagcttca acttggtcgt gtgttacata gataaatgta
    20581 gttccaagct gttggtgtag cttagttatc tctgttctca tagcagttct aagttttgca
    20641 tctaagttgg atagaggttc atccattaag aataccttag gatttctaac tattgcacgt
    20701 cctaaggcaa ccctttgtct ttgtcctcca gataatgttt taggtttttt atttaaaaga
    20761 ggtagtaagt ctaatatttt agcagcttct ttaacttttt tgtctatttc atctttaggc
    20821 aaatttctaa gtttaagtgc aaatgccatg ttttcgtaaa cagataagtg gggatataaa
    20881 gcatagtttt gaaaaaccat agctatatct ctatcttttg gttctatatt attgacaagt
    20941 ttatctccaa tgtatagttc tccttcagag atttcttcaa gaccagcaat cattctaagt
    21001 gtagtcgatt taccacaacc agatggtcct actaaaacga taaattcttt atctttaatg
    21061 tctatattta tattttttac tgcgttaaat ccatttgagt aaagttttga tatatttttt
    21121 aaaattactt ctgacataat atcctccttg atatttaaat tttaattata tagtatcaaa
    21181 tttttttatt atattaaatg tgtaaaatga ctaaatgtat ttgtttaata tgtatattta
    21241 gtttttagca aagaataaaa ctaacagtag aatatattta aaaaattaaa aaacaaaatt
    21301 aaatttatta tcaaaaaatc attttatgat atgttttatt tttatattac aaatagagaa
    21361 taatgaatta tactagaaag taggaggtga aaatatgagc agaatttcaa tttttatgga
    21421 atcatgtaat ggcaaatttc caaatgaaaa ttcaatgttg tcagtttcct ttataaaaaa
    21481 tattttaaat gcagaaaaca aagattttgc taaaaacata tttaactatg gggaagaaag
    21541 aaaaaacaat aagcaaataa aggatataaa tacagccatt tacataccaa atcttataag
    21601 aagcaaaaat gagttacttg ttgatggaga tataatattc aatataagtt tttataatta
    21661 ttctatgttc agtaaattat acaatggaat tttaaaagta aaggagctag aatataaagg
    21721 ttatagattt aaaataaaaa atataaaaat acataaagaa cacgagataa aagaaaatgg
    21781 tgttatattt aaaactatgt ctcctataat agtaaagaat aaagaaggta aatatttaga
    21841 tgtagaagat tcaaattata tagaatgttt aaactatata gctaatctaa ctctggagag
    21901 tataagaggt agtgggctta gaaagccatt ggaatttata cctattaact tcaaaaaaag
    21961 agtgttaaaa gaaaagattc gtggatttaa agtgagagaa tattactata taaatgctta
    22021 tgcaggaaca ttttttttaa aaggagatat ggaagattta aatgcactgt ataaaatggg
    22081 aattggatat agaaggacag aaaatgcagg aatggttgat atattaaagt agcgaggtga
    22141 gagaaataaa agctaaattt tatagatcag gtgatattgt aagagatatt ggcattgttt
    22201 acttttatga attactaatg gatttaaaaa acgaactaga aaaaaatgat tataagctaa
    22261 aatttacttg tgagttaaat agaaattatt tatctttaga atctgcagaa tatatagacc
    22321 ctaagtatat atctgattat atattagaaa atcgactttt taaggtattt aaaaatggga
    22381 aaaaggaaac cttagaaaaa atatttagta atatagataa tttaacgtat gtaaatttta
    22441 ttgaagaatt agataaatct tctgcaactg ctaaacaaaa ggaagatatt aaaaaagatt
    22501 ttaaaaacag gaggtttcca tatgttagaa atagtggtaa gtatggatta aacacgagtt
    22561 tagaaaatat ggaaacaaat gtaaaaagaa tagtagatac tgtaattaaa tcaaatatag
    22621 agatagatga aatagataaa cttgatttga cacaatatga agaaaaagat agtgtatgta
    22681 atgtttgcaa tataaataaa acaacaaaac tagatattga tttaagagaa cgaaaggact
    22741 ctaagtataa ctttttattt agaggtgcag aaaaatcagg ctttaaaaga agtggacaag
    22801 ttgaaagtaa tatttgtttt gagtgtgaat ttttcaactt aatgtgtctt ttatatataa
    22861 atttaaaaag accaatggtt tttgcatata cgaatgattt aagagaatta gcatttttaa
    22921 atcataagat aatgcttaaa agaaaaatgt attcagataa atcattttat aaaaaacttt
    22981 tgcatgaaaa aataagttca attagactat atagctttga tattgatacg aataaaggta
    23041 taattctaaa gtttgattct attatagagt ataaagaatt attaaaaaaa attgaactta
    23101 tagatattat agataattat aatttttcaa gagagccagg taatactaga aatttgggta
    23161 aaagaatgat tgataatggg aatttatcaa atttaaaaga attattactt gataatataa
    23221 gtgttttaaa gcaatcaact ggaacttctc gagaaatgga ttttgtatct agctcatata
    23281 atatcggttt atatataaaa ttgtgttgga ttgtagatgg aggggaggag tataaaatga
    23341 aaaactatat ggaaagtaat ttaatatatt caagagtagg taaagactta tttaataaaa
    23401 tgacagatga atccagaaga aaaaattttt ctatgaaagt gattcaacta cttaagtcta
    23461 atgatagaac tcagctattt caaactataa tgcatgtgtt agtttctaat ggaatcttaa
    23521 taggagaagg gtttgtagaa ggtctaatgc agagttctga attagaatta aatacgaatg
    23581 taggtctatt tattcaagaa atcatgaaat aaaaaagggg gaaaatttaa aatgaatgaa
    23641 aaaagagtaa aaggagtaac attaactata ctgacagaaa gcccagttcc actttcatat
    23701 gaccaagggt atggaaatta tacaccaata aaaaaagaac aatatagaga aaaaatacat
    23761 gcaaaaacta gtatagccac aataacatat gatttaagaa gaatgctcca ccaagaatat
    23821 ggttggaatt taagtaatat tgtttttgga aaaaaaggaa atatatatcc atctattaag
    23881 aaaaatgtag gatgtgtaga tgaaaatgga ttagagactg atgtatttgg ttatttaata
    23941 ccacttaaag ataaaggttc tataagtaaa gcttctccac ttagaataat accttttagg
    24001 tcattaaatc catataaagg ttcaacacag ctaataacaa atagaggctt tttaagtagt
    24061 gaatttggta gaaaatatta tgatgagaag gaagaaaatg aagtacctag agatgaaaac
    24121 tttccaacat cacaagcatt agcaatagaa gagactttat cagattacta cgtttataca
    24181 ataacattgg agcttgatag aataggagta gtagaagtag aagatggaaa gttactttta
    24241 ccagaagaaa gaaaatttat gagtaaggag ttaagagaaa aagcagtaaa agatatatta
    24301 gatgcaatta ctgtgtttac aagaaacata aaacattcat cagtactatt aaaaccactt
    24361 gcagtaatag gtggagcttt tgataaagtt gtaccatttt tttgggatga tgtagattat
    24421 aatgctgata gtggtgagat aaatttagag ggagtcatag aaactataga aagttatagc
    24481 cttaaagaaa gtaacacaat acttgctatc aatgatagat taaaaatttc taataaaaag
    24541 gaattaaata agtttaattt aagtaaatac cctgtaaaag agattaaaaa tctagcagat
    24601 agattggaaa ttggagaaga taatatgtgg tatttaaaag agtaggaggt tttttgaatt
    24661 atgaaagtat tacaatgtaa acttaagcaa ccaactgctc actatagaga cccaaaagta
    24721 tttcaaaatg aatatataag tactttaaat ttaccatcta agaccacaat aatgggaatg
    24781 ataacgtatt tatgtgatag aagattaaac tctgatatag atataggaat aattggaaca
    24841 catcatcata gagaactaga attttcaaga ggagaaaaca ttgatttttg gaatgaatat
    24901 ataaaaatgg gaaaaggtaa agataaagaa aagtttttgc ttaaaggaaa ctactatgat
    24961 tactataaag agcataaagc tcaaaatagc atattaaact atgaagttct taaagaagta
    25021 gaactgacta tatttatttc ttgcaaggat gatgaagaac ttgaatttat tagaaaaaag
    25081 cttgaaagtc catgtaaata tgcaaattta ggaagaaaag aagattttgt aataccaagt
    25141 gaaaaagggt gttttgtgaa agaagttaag ttaaaagagg ttatacctat gaatactcgt
    25201 gatgctataa aagaaaatat aaaattaaag aatacatatg taagagttga cttaagagat
    25261 aaagagaatg tcgagactat aataaatcaa ggtgtattga ttgcattgcc tcataaatat
    25321 aaagatttag aagcaaatag agatgataga gtttatgagt tttgccatta tatatatgtt
    25381 gataatgatg gtatatatcc taaaaatatt aaggtaaatg tatgtattga tacaaaagaa
    25441 gtttttacat ggctttaaat tattttgaaa gataggtgtt tatcatgaaa aatatcatgc
    25501 aaaaagaaat attagctaag tcatacaatt taagaactaa tcaacaagaa caaacattag
    25561 ttgaacatat taaggattta tttgaagtat tagaatctat attagaatta aatctatatt
    25621 cagataaaga tgtagagata ctaaaaatat gttgtgcttt gcacgattta ggaaaaatta
    25681 attcaattat gcaacaaaaa atggagatta ataataaaat atcgtatagt tgctcagaag
    25741 aagagaggaa aaagttagag tctgacaaaa aatctttaaa aaagatagca agacataata
    25801 tattttctgg agcattttta aaagatatat tagaaaaaat gaacttgtca gaagaagata
    25861 agttatatat atataaaagt ataatgttac atcatggaaa ctatgaagat tatatgaggt
    25921 taagtattag taaagttcaa gaagaaattt atgagtatat tgaaaaaggc attttagaaa
    25981 aagaagagtt taatctgaaa gatatagaaa gttatataaa tagcatttta aatgtagact
    26041 ttaaatttgg tgaagatgta ttggattatg attacataga taaattaagt gaaagtatgt
    26101 ttatagacag tgattataac aatggtcaaa tagatgattc agttttaaat tatagaaagt
    26161 ttaagtatat tttatataaa gggatgctga acttaataga ccatagtgca tcaagtagac
    26221 agaaaggtat aaaattttat aacgatttta ctgatgaaga aattgataat atgattttaa
    26281 atgagatata taagagtcag ggtaatttgg aaaaaaataa tatagagttc aatactatac
    26341 aaaaaaggtt gagagaactt tctggaagaa atgttctgac tgttgcattt acaggttcag
    26401 gaaaaacagc tgcagattat agaaagactt ttaaaagaaa attcttttta gtgccaaata
    26461 aaatatcagc agaaagtttt tatagaaaaa atatttttca aaataaaaat gatttagata
    26521 caagaagtag taatgattac attggattac ttcatggaga tataaactta tattcagaga
    26581 atgaagataa tggagaacat gattttgtat tgacattaag ggatatagat ttgagtataa
    26641 acttttgcaa accttgtgta attgcaacag ttgatcagtt attacttagt atgtttaaat
    26701 ttccgggata tgaaaaaata ttcgcagccg taaaaaatgc atctataact gttgatgaag
    26761 tacatttatt ggaaccaaag atgtttttga ttttgatata ttttatgcag tttgcatgca
    26821 agtacttaga tgttgatttt cacttaatga cagctacact tccaaaatca tataaagagc
    26881 aaatgataaa caagggaatt atttttcaag aagaaagtaa tgaaaatgtt actgataaag
    26941 gagaaatagt ttttattgag agcaacaagg aagaagagat ttgtgaaggt aaagatgtaa
    27001 aggtttcttt tataaaggaa aaagaaatca agtcaatagt tgaagatgct ttagaaaata
    27061 aacaaaaaat tcttataata aagaatacta ttgattcagt caatagaaca tatgagtttt
    27121 taaaagaaaa tttatctgat aagtatggtg gtgtagatat agatgtttta catagtagat
    27181 ttaagttcaa agataaaaaa gagaaatata gtaaaatatt aaatggagag ggtgatattt
    27241 ggatatcaac tcaatcagta gaaatatcct tggatttgga tttcaatata ataataagtg
    27301 atttagcaac tatggatagc ttaatacaaa gaatgggtag atgcaataga aataataagt
    27361 atgaatatgg aaacttttat attctaccta gtgaagataa aatttatgac aacaaactta
    27421 agagcacaac taaaaacatc ttaaaggaca tattaaaaac taactctatt tttacaatgg
    27481 gaattagaaa aactattttg gatgattact atgataatag tgtagtcaaa aaatattttg
    27541 aagagaattt tataagttgt gataaagaaa ttaagaatat ttatggtgta aataaagaaa
    27601 tttttgatgg attggattta atttttaatt ttgaacctta caaaaatatt gttgatagta
    27661 agaaggaagc agctaaaatt tttagagatg ttgatgttag ttacaaaatt attctggaag
    27721 aagattttta taaggaagat agagagttac aacaagattc gattcaagta tcagggttta
    27781 tatttaatag attatattat tatgggttaa taagcaaagt agaaggatac atggtactaa
    27841 aatctagtag taagtttgag tataatagta ctgtaggctt aattttgaag tgagcatatg
    27901 tttaaatata atataccttt agacattgaa aatacaaaaa gtactactgt aagctcactg
    27961 taaaaactat tttttaaaag gaaaaataag ttgtgagata gagtgtttac aagtgtatat
    28021 agataataaa tttgaggttt aagattaact aagtgggttt caggtacatc gcgaaaataa
    28081 ttataagttg gagattttgt atgtttaaga ttaactaagt ggatttcaaa tattgatata
    28141 gtagatataa tagaagtttt attattaggt ttaaaattaa ctaatcggat ttttaaatat
    28201 tctgactatt atataaggat attattagat tgaatttaaa accaataata taaaacaaca
    28261 aaatattata tttatgatat aataaaagtt atggaaaaac aggaatatta ctatagagaa
    28321 gagaggtaaa cttatgaata attggataga agtaactata aaaacaacaa cagaagcagt
    28381 tgaacctata acgaatatac tatatgaaca aggagctgga ggagctgtaa tagaagaccc
    28441 aaaagacttt ttattccaaa agaaaaatga actagactgg gattatgttg aagaagaagt
    28501 atttaaaaag aatgaagaag atgatgttct tataaaaact tatgtttctg aagaaaaaaa
    28561 tgtgatggaa tttgtcgaaa taataaagca aaaagtttta ggattaaaag attttggtat
    28621 agatataggt gaaggaagtg tatcattgga ccaagtaaat gaagctgatt gggcaaatgc
    28681 atggaaagca tactacaaac caacaaaagt aggacaaagg gtagtagtaa agcctacatg
    28741 ggaagattat gctatgcaag agggggattt aatcatagag cttgaccctg gaatggcatt
    28801 tggtacaggt acacatgaga ctactagtat gtgcattaga gaattagaaa agtatgtgaa
    28861 taaagattct aaagtatttg atattggatg tggtagtgga atccttgcaa tagcagcagc
    28921 taaattagga gcaaaagaag ttgtagctgt agatttagat gaagtggctg taaaagttgc
    28981 aaaagaaaat gttcttgaaa ataaagttga aaaaagtgta tctgtaatgc atggtaattt
    29041 aactgatgta ataaaagata aagcggatgt aatagttgca aatataatag ctgatataat
    29101 aaagatactt gcaaaagatg ttcaaaactt tatgaaagaa gatgctatat tcatatcttc
    29161 aggtataatt ttggataaag tagaagaagt taaagaaagc ttaattgaaa atggatttga
    29221 aatagttgaa gttcaaaaat taggtgaatg gtcagcaata gtttctaagc ttaaaaaata
    29281 agggatatgg tgacgtataa tggatagatt ttttgttgaa aaaaataata taaatctaca
    29341 agataaaact tgcactatag aaggtgaaga tgtaaaacat atttcaaaag ttttaagatg
    29401 caaattaggt gagaaacttg aaatatgtga caagaataat aatgagtata tttgtgagat
    29461 aatgaatata gataagagta tagttaattt agaaatttta gaaaaagttg atataaatag
    29521 agaatcagaa cttaaagtaa ggctatacca gggacttcca aaagctccaa aaatggaaat
    29581 aatacttcaa aaattaactg aagtgggtgt agaagaaata atattagttc aaactaagag
    29641 aagtgttgtt aaagttgatg acaaaaaaga agataaaaaa tttgaacgtt gggaaagaat
    29701 aatttatgaa gcagcaaagc aaagcaaaag aggtaagata ccaaaattaa gaggtgtttt
    29761 aagttttaaa gaagctttag aagatatgaa aaaaaatagt gtaaatattt gcccttatga
    29821 aaatgaaaga acagtttcaa taaaacatgc actaaaaaaa tgtgattcta atatagatag
    29881 tgttggaatt tttataggtc cagaaggtgg attttctgaa gaagaaatag aacaaataca
    29941 aaaaaataat tgtaatgttg tatcattggg gcctagaata ttaagaactg agacagcttc
    30001 tgttgtagca tcaacaatag ctttgtatga actaagtgat ttaggaggag aaaaataaat
    30061 tgaaaaaagt agctttttat acacttgggt gtaaagttaa tcaatatgaa acagaagcta
    30121 tgctagaact atttgagaaa gatggttatg aacaagtcaa tagtgaagag tatgctgatg
    30181 tttatgttat aaatacatgt acagttactc atatgagtga tagaaaatct cgtcaatata
    30241 taagacgagt taaaaagaag aatccagatg caataatagc cgttgtagga tgttattctc
    30301 aggtatctcc ggaagaaatt ttggatatag aagaagtaaa tttagtcatg ggtacaaatg
    30361 atagaagaaa gattgttgaa gaaattaaaa agataaattc aagtaagaaa gttagtacag
    30421 ttgatgacat aatgaaagtt aaagcttttg aagaaataga aatcagccaa actaatggaa
    30481 agactagagc atttatgaaa atacaagatg gatgtgacag attttgtact tattgtatca
    30541 ttccttatgc ccgaggacgc gttagaagta gagatattga tagtatagtc gatgaagtta
    30601 aaaaattggc taataatggc tataaagagg ttgtattaac aggaatacat gttgcctctt
    30661 atggaaaaga tttaaaggat agggatatta aacttcttga tgtaataaaa caaataaacc
    30721 agatagaaaa aatagaaaga attaggctta gttctgttga accaatttta tttacagatg
    30781 aatttgtaaa tgaagtttta aagatggata aagtttgtcc tcattaccat ttatcacttc
    30841 aaagtggatg tgatgaaact ctaaaaagaa tgaatagacg atacacaact ttagagtaca
    30901 aaactattgt agatagattg agaagtaaaa tgccagatgt ggcaataact acagatgtaa
    30961 tagtaggttt tcctggagag acaaatgaag agtttaaaaa aacgtacgag tttttaaaag
    31021 aaatagagct ttcacaaatg catattttta aatattcacc aagaaaagga actccagctg
    31081 caactatgga aaatcaagtt gaccctcaaa tgaaacactt tagaagtgaa caacttttaa
    31141 atttaagtaa agtaaatttt aataaatttg caactaaatt tatcggccgt gagttggatg
    31201 ttttatttga acaaaatata gaaggcaata aatatgaggg attaacttca aattatataa
    31261 gagttgttgt tgaaagtgac aaaaatatac aaggtcagat attaaaagtg aaaataaatg
    31321 atgtaaaaga tgaatatgta gaaggaattt tgctataaca tgtagaaatt ataatattta
    31381 taagatagga ggtggaaaga atggattgta ttttttgtaa aatagcgaac ggagaaatac
    31441 cttcaactaa agtttatgaa gatgatagag tcttagcttt taatgacttg aacccagttg
    31501 ctccatatca tatattagtt gtacctaaaa aacattatga tagtttaatt gatattcctg
    31561 ataaagagat ggacattgtg tcacatattc atgttgttat taataaaata gctaaagaga
    31621 aaggttttga ccaaactgga ttcagagtca taaataattg tggaagtgat ggagggcaag
    31681 aagttaaaca tttgcactat catattttag ctggtaaaaa gcttcctaat tacgaagcgg
    31741 gacaaaacta aactaaatta ttgaaaaatt gagttccttc ttgataaaaa tacttgataa
    31801 aagtaaaaat ttagtttata ataaacaaag tgttaggttg taatgcatat tatgcaaaaa
    31861 gaacatagtt acagtcccag cataattgcg ctattggagg gagggaaaaa gaaatgtcag
    31921 aagtaagagt tagagagaat gaaacattag acagcgcttt aagaagattc aagcgtcaat
    31981 gtgcaatgtc tggcatcatg tcagaagtga gaaaaagaga gcactatgat aagccaagcg
    32041 ttaagcgtaa gaaaaaagct gaagcagcaa gaagaaaaaa cgctaagaaa tagtagtaaa
    32101 taagaataaa gaggtgaagg ggatgtccct taagcaaaag ttacaagagg atttgaaatc
    32161 ttcgatgaaa aacaaagata cagtgagaaa atctgtagta actttaataa gagcttctat
    32221 aaagcagtat gaggttgata atagggtcga gcttgatgaa gatggaatta tcgatgtaat
    32281 agcaaaacaa ttaaagcaac gtagagatgc attagttgaa tttgaaaaag ctggaagaga
    32341 agacttaata aaagaaacag aaggtgaaat tgaagtttta aaagagtacc tacctcaaca
    32401 attaagcgaa gaagagttag aagaaattgt aaaatctact atatccgaag taggggcaac
    32461 ttctatgaag gatatgggaa aaataatgtc agttatccaa ccaaaagtta aaggaagagc
    32521 tgacggaaaa cttataaata agttggtaaa acaaaactta caatagattg atatagccta
    32581 gagagaaatc tctaggcttt ttatagtttt tgggaataac gagtcatatt tgagcatatc
    32641 tataatcaaa gaaaattttg aaagaagggg aaaaatttga ttatatcagt aaaagagatt
    32701 aggaattttt tgataaacat atttatacca ctaataatag gttacttaag tagcatctta
    32761 acaatgatta tcagtggaac agatatttca acctattatt tacaactcac aaagccaagc
    32821 tttgcaccac cagcttttat ctttcctata gtttggacta tattgtacat attgatgggt
    32881 atatcttcat acctaatatt aagaaagggt tacaacttac ctaaagtaag agatgcaatt
    32941 ttttattatg gattacaact tgtattaaac tttatttgga gtatactatt ttttggattt
    33001 ggacttagat ttacagcttt gatagatata ataataatga ttttggtttc tataataatg
    33061 atttcgaagt tttctaaaat agataagaga tctggataca taaatttgat ttatttaatg
    33121 tggcttgttt atgcaggatt tttgaactat tttatatggt ttattaataa atagaatgta
    33181 aagattttgt aaattaccct gtacttttaa taatatcata acaaacgaaa aataaataga
    33241 ctattaaaaa taatcatttt catatacaat aatattaatt taaatacaat aaatttataa
    33301 aaattgtatt taaagattaa catattaaag tattagaatt ggaaaggctt gaagggagag
    33361 gtttttatgc aaataatagt atctgggaga caaatgaaat taactgacgg aataaaagga
    33421 tacgtagatg gaaaattaag taggttagaa aaataccttg atcctgaatc agaagtgaaa
    33481 gtaacagtaa gtgcgaaaaa ggatagacaa aaagtggagg ttacaataat tcctattaac
    33541 ggtcaaataa taagagcaga agatgtagaa gatgacttat atgcagcaat tgatattgtt
    33601 tgtgacaaat taagtagaca agttgtcaaa tataaaacta aagttaaaga caaagttcaa
    33661 aataataaga gcataagatt tgaaaatctt gactttatag ataatagcag tgaatttgat
    33721 gattatgatt atgatgatga agaagatgaa aatatagtaa tagaaagaag aaagaaattt
    33781 aatgtaaaac ctatgagttc tgaggaggcg atacttcaaa tggaactagt aggacacaat
    33841 ttctatatgt ttagaaacca agataatttt gaaataaaca tagtgtataa gcgtaaagct
    33901 ggtggatatg gtttaataga acaagattaa taaaaattgt atacgtttag attttatata
    33961 agaaaaaata aaaaggtaat catgttgatt ttttatcaat atgattacct ttttcttgtt
    34021 taaataatat attagatatt aggtatttat tatataaaaa ttttaaaact aatctgttac
    34081 aacatcaata aagtgcataa atacctattt caagcataaa attaattaag ggggtaatgc
    34141 ttatgttaga aatatccact gatttacaag taaatcaacc agttattact gttacaagta
    34201 atacgtttat tagtatagaa aattatctat caatattaga atatgaagtt gatttaataa
    34261 gaataaagac taaagttaaa acaattaaaa tatctgggga taaactttca cttaagtata
    34321 ttactgatag tgagataggt ataaaaggga ttatctacaa tgttgagtat gttgattagg
    34381 aggagataca cttgataagt tttataaggg gatactatgt tatagttgtg gaaggagtag
    34441 gactcgaaca atttttaaat catcttataa gaaatgggat aagtgtttac aatgtaacta
    34501 gaataaaaaa tactaaaatg gagtttcata tagatagaca agatataaaa gactttaaga
    34561 atgtttatag aggaagtaag tttgatataa aggtaaagca aaaaactggg gtccctttta
    34621 ttatcaaaag agtttataag cacaaaggta tgtggatttg tgctcttgtt tctttatttt
    34681 tattgatgtc tacatcacag tttgtgacag atgtatatat acaatctcct gaaggtataa
    34741 aaaaagaagc tcttagaaat gagctttata aggttggagt aaggccagga gtctataaga
    34801 agagtattga tagaaaagaa gttagagacc atatgatgtc aaaatttaat gatgtggcat
    34861 atttatctat caatgtaaaa ggaacaaata tatttgttac tgttacaaaa aaggcagaat
    34921 ccctaaaatc tacagaccaa tcaaattatt gtaatgtaat tgctttaaaa aatggtataa
    34981 tagaaaaggt aatccctaga agtggtaaat cagttattaa atctggagac atagtccaaa
    35041 agggagatgt cttattaaat ggagctaata ccaagtcaat accagaagta tgggcaagta
    35101 ctttttatga gtccacaaaa aaggcaagtt atgtagatac tgtaaataaa aaaactggag
    35161 aaaagaaaaa tatatataca ctaagttttt atgataaaga gtttacaatg agaaaaaata
    35221 taaaatataa agattatgtt gttgaaaata aagaaaaaaa actatcaata ggaaactata
    35281 cttttccaat aaagataaaa acgagtactt tttatgaaac aaaaaaggtt gaagtaaaaa
    35341 ggaataaaga agagttaaaa aaagaattat ctgaaaaagc tttaaaagag ttagaataca
    35401 taatcccagc ttcggctaga ataatagatg taaaacataa ctttaaggtt aataaaaata
    35461 tgttagaata tttaataaca gttcaaacta gtgaaaatat tgctaaaatt tatcctctta
    35521 gtaagtcaga agcagaaagg tttataaagg aagaaagtaa accagatgaa ggtgaagagg
    35581 aagttccttc caatccagaa aaaagaccat taaatgatat aagaaatgag tttgatgagg
    35641 ataataagga taataaagac caaaacaatt ctgatgaaaa taatagtaat caaaatagta
    35701 ataatagtca aaataataat agcaataaca actaataaaa ttaaacttaa aatcttagga
    35761 ggactaattc aattgataat acaaaagaaa tttacaatag cagatggtaa ctttgaaaga
    35821 gagttatttg gcaactttga tgaaaatgta aaattaatag agaagacttt aaatatagat
    35881 gtaatactta gagaaggcaa tataatttta attggagaag aaaaaaatgt agattctgca
    35941 ttaaaactta tgaatgaatt acaccaaact gtatcaaatg gtaagcattt agataagcag
    36001 agtatttctt attcattatc tcttttgctt gaaggaagtg aacaaaaaat aaaagaactt
    36061 gaggggacaa tcgtcataac tcaaagggga aaagctgttc aacctaaaac tttaggtcaa
    36121 aaagaatata taaaacttat agaaaataat gatataactt ttggtgttgg acctgctgga
    36181 acaggaaaga cttatcttgc ggttgctatg gcagtaaaag cttttaaaag agatgaagtt
    36241 agtagaatta tacttacaag accagctgtg gaagcaggag agagtctagg ttttttacca
    36301 ggagatttaa aggacaaagt tgacccatat ttaagaccac tatatgatgc cttatttgaa
    36361 atgttgggtg ctgataaatt taataagtat ttagaaagag gtactattga ggttgcacca
    36421 cttgcattta tgagaggtag aacacttgat aattcattta taatattgga tgaagctcaa
    36481 aatactactt ctgaacaaat gaaaatgttt ttaactagac ttggatttgg ctctaaagct
    36541 gtagttacag gagatgtaac tcaaacagat ttaccacaaa ataagaagag tggtctaata
    36601 caagctacag agattcttaa aggtgttcca ggtattgggt ctataatgct aacagataga
    36661 gatgttgtaa gacatgagtt ggtacagaga ataataagag cttatgagaa gcacgacaaa
    36721 agagaagaat ttaaaaaaga agaacgtaaa aaagcgaaaa tgcaggaaaa gactttcaaa
    36781 agaaagtaaa agtgtagtaa tatagatata tagcattatt aatgaataaa aagtaggtgt
    36841 agtgaatgga tttgatacta gatgatagac aagataaatt agaagtaagt gaggagttaa
    36901 ttgaaaagat aaaggatata atcatagaat gtttagatta tgaaggatat gatgataatt
    36961 atgaagtgag tctttctttt gtagataata aagagattca tgaattaaat agagaatata
    37021 gaggagttga tagagtgact gatgttttat catttcctct tttaagtgat gattttgaag
    37081 atgttgagtt agaagaagaa tccctaggtg acatagtagt atctcttgaa agagccttag
    37141 aacagagtat agagtataac catagttttg aaagagaagt atgtttttta atatgtcata
    37201 gtatgtttca tcttttaggt tatgaccacg atacagatga aaatacaaaa gaaatgagag
    37261 aaaaagaaga acacatatta aataagttaa acataacaag ggagtagttt ttatgaaacc
    37321 agaaaaaact cgtcaaggta taattaaagc atttaatgca gctatagaag ggatattgta
    37381 tacatttaaa tttgaaagaa atatgaaaat acattattta ggttcagttg ctgttcttat
    37441 aataagtttg ttttttaact tttcaaaatt agaaatgata atgttactta tgtccatatg
    37501 tttagttgta gttgctgaaa tgtttaatac agctatagaa aaagctgttg atttagtaac
    37561 tgatgaatat catgttcttg ctaagatagc aaaagatgtt gcagctggag gcgttctagt
    37621 ggcagcacta aattcagtag tagttggata tattttgttt tatgataaat tgacagatat
    37681 atctggaata ttaatttata aaataagaga atcagaatta catataacct tgatatgtat
    37741 attattggtt cttatagctg ttgtagttgt aaaagcattg acttctacag gtactcctct
    37801 aaaaggagga atgccaagtg gtcatgctgc cttagcattt gctatagcaa ctgcaattac
    37861 acttatgact gaaagagttg ttgcctctac tcttgcatat ataatggctg tattagttgc
    37921 ccaaagtaga attgaaggaa aaatacatac attttgggag actattgctg gagctttgtt
    37981 aggtgtgttg atagctatat tagtatttca gcttggaatg ttctataatt aaaataaatg
    38041 tacataaaat atttaaaata aaacaaatct gtgctaatta aatttttatg atataatgta
    38101 gagtattaaa taaaaataac ttttaattat tgtatacagg aggagttgtt gtggataaca
    38161 tagaattgtt aaggttggca gaagatgcca gacaacattc ttatgctcct tactcaggtt
    38221 ttagagtagg tgcagctctt ttaactaaga gtggtaaagt ttatactgga tgtaatatag
    38281 aatgtgcatc attaggtgga acaaattgtg cagaaagaac agctattttt aaagcaatat
    38341 cagagggtga taaggatatc tataaaatag ctatagcaag tgataactca gaaaataatg
    38401 agcaaacata tccatgtgga atatgcaggc aggtaattat agagtttggt tctgatataa
    38461 aaataataac gggatatact aaaggagagg tgtttgagca tagtataaag gatttgttgc
    38521 caaactattt ctctggaaat gatttaaaat aaatttaaaa gtataaataa atttaagtaa
    38581 ttaaaacaag aagataaaaa gttaggagac agttaatgtt caaatcaggt tttgtcagta
    38641 tagtgggtag accaaatgta ggaaaatcta ctttaatgaa taatgtagtt ggagaaaaaa
    38701 tagctataat gagtgataag cctcaaacta caagaaatac aatacaagca gtatatacag
    38761 atgaagaaat gcagatagta ttcttagata caccaggtat tcacaaacca aaaaataaat
    38821 taggtgaatt tatggtaaag gcagcaacag aagcttttaa aaatgtagat ttaatattat
    38881 ttgtagttga tgattctaaa aaaataggtc ctggagatag aaagataata gaggatttaa
    38941 gaagtgttaa gacaccaata attttagttg taaacaaaat agaccaacta gaccaaaaag
    39001 atgagctatt tgatataatc aaaatgtatg atagagaagg tatttttaaa gaaatagtac
    39061 caatatcagc attaaaagga aagaatacgg ataccttaat aaaagttata caaaattatt
    39121 tagaagaagg gccaaagtat tttccagatt atatgattac agaccagcca gaaagagtat
    39181 taatagcaga acttataaga gaaaaagttc ttcactatct taatgatgaa atacctcatg
    39241 gtgtagctgt tgaaattgaa aaaatgaaag cgagaaatga taaggaaata gtagatgtat
    39301 ctgctgtaat atactgtgaa agagattcac ataagggaat aataatagga aaaaatggta
    39361 ggaaactaaa aggaataggt aagtcagcaa gacaagatat agaattgctt ttaggttcac
    39421 aaataaatct tcaattatgg gttaaagtca aggaaaattg gagaaattta caaaattata
    39481 taaataattt tggatataat gataaataaa ggtgggttat atggatagga aactggatgc
    39541 ttcccaagat ttattatatg aagtaaaatc attaattgat aataataaag tattggagtt
    39601 aagagaatta atagaggaat atcatataat tgatatattc gatataatgg aaaatttaga
    39661 ggaagacatg aaaattcaat tatttgaagt acttccttta gatatggcat cttctatatt
    39721 agaagaaggt agtgtagagt tttttattag tattttatct aaactggatg tagaacactc
    39781 gaaaaatata cttgaactta tgtctcttgg agatatggca gacaagttga gtgaacttga
    39841 agaagaagaa agagaacaca taataaatct tttgaatcaa gagaatgccg attatgtaaa
    39901 agagttacta ttctatgatg aagattctgc tggtggaaca atgaccactg gatatatttc
    39961 tataaataaa gatatgacag ctcttgaggc tatagaccat atgagagaag aagctgaaga
    40021 agctgaaact atttattata tatatgtggt tgatgatgaa gaaaaactag ttggagtatt
    40081 gtcattaaga gaattaataa tagctagaga cgcaaatata gtagaagatt tgatgagtga
    40141 aaatataatc tctgtttatg tagatgaaga tagagaagaa gcagtaagat tagtatctaa
    40201 atacaattta atagcaattc cagtagtaga cagacaagaa aaattaaaag gaataataac
    40261 agtagatgat ataattgatg taatggaaga agaagcaact gaagatatgt ataaatttgc
    40321 aggaagttca gaacatgaaa gagaagttgc ggaaaaagaa aaccctacat tgagagagca
    40381 aataatatca gcacttagag gacgattacc ttggttaatt attaccttag ttggagggtt
    40441 gttggcttcg ttaatacttt ctaacttaga ctatataatg aatcctgtat atgctccatt
    40501 agtatttttt atacctgtag taattggtat gggtggaaat attggaactc aatcatcatc
    40561 tgttacagta ataacattgt ctaataagga tttaaatttt agcaatgtgg ttagagaagg
    40621 tattgtaggt attattacag gtcttttgtg tagtataata acaggtattg taatttactt
    40681 tgttatgaga aagttagaca ttgtattaat tgtttcaata tcattattta taaatatggt
    40741 tttaggtgca acaataggtg cgtttatgcc tgttttatta aaaaaaatgg atgcagaccc
    40801 atctacagtt tcatcaccaa ttatatctac agctcttgat ataactggta tagcagttta
    40861 ctttataata acaacggcat tattgtcaaa aattgtttaa taactttatg tatgatttat
    40921 cctcttctta ctaaaaatat aagtataaat gtaagaaggg ggttttttaa aaatgaacaa
    40981 tatcagttgg gaagttttta aaaaaacagg tagtatagat gcatatttat atttctgtga
    41041 ttgcaaaaac atacatgaag aaattgaaga agtaagggag aaaaaagaag acgatgataa
    41101 tcctgaacac ccaggggata gtacttaaag ctataaggta taaggaaagt gatataatac
    41161 ttacactatt tacaagaaag cttggaaaag tatctgcaat agcaaaagga gctaaaaaaa
    41221 ataagagctc tttgctttca tcatcacagt tattttctta tagcaatttt accttaaaaa
    41281 aacaaggtaa tatgtacaag gttactcaaa gtgaaataat aaaaagtttt tataatatat
    41341 cctatgatat agaggcattt tcttatgcta catatattac aaaattagtt gaaaattcta
    41401 tacttgaaaa tcagactaat aataggctat ttatattact tgcgcagact ctgtatttgt
    41461 atacacagga taatacagac aataggttta taacagcagc attcgagtta aaatttttag
    41521 actatatagg atttaaacct atagtaaata aatgcattaa ttgtgaatgt aaaattttac
    41581 aaaattctgt gtttaatatc tacgaaggtg gtatactatg tagtaagtgt agtaaacttt
    41641 ttgataataa tataaaatta gacttaacaa ctataagtct tatggaatat gtattaagaa
    41701 atgatatttt aacatgtagt aaagctaaag tatcaaaata tataacacat gaacttgaga
    41761 atattctaga caagtatttg aaagtttatg ttgataatat taattttaaa tcattacacg
    41821 tattgcaaag tgtaaaaaat aataagggag tggataatga tgagtagtaa tataactatt
    41881 gaaatggttg attcagtctt tgaaagagtt ccaggagcaa cttatgcaga agcaaaagaa
    41941 gcattagtaa tgtgcaatgg agatgtaatt gaggcagtaa tatatttaga aaataaaaaa
    42001 aatacttgta attgtaaagc aaaatctgct aaagaaacta tggaagaagt atttggcaaa
    42061 gatggagaaa atataaagtt tcaattaaaa gagcttctta gaaaaagtag tgtagtgcga
    42121 ataatagttg aaaaagatgg aaaaactatg atgaacattc ctttaacagt tggagttgtt
    42181 ggattagctt ttgtaccatt agctacttta gttggattat cagctgctgt tataagtaaa
    42241 tatcgtataa aagttcaaaa tgaagaagat ggagaaattg tagatttagg tgaattgaat
    42301 gaagaaaaac ttcacgtttt gaagaatatg ataactaatg cagcaaaaga tgtgaaggat
    42361 gtagtagttg acaaaaaaga agatgacaaa gatgtaactg ctgaccttat gaaagaagaa
    42421 tctgaaaaga ataaagaaga cgaagacgaa aataaataga tataaagtga cttttaaaaa
    42481 atgaagagaa gataattata agaattatca ctaacattga caaattttat agaatttgat
    42541 attctaaaag ttgtagaaga actattaaaa tagcctttcg tgtagacgag aggctttatt
    42601 gttataggag ggatttttat gaattttcaa gagatgatac tggcgttgca aaaatactgg
    42661 tcaaagcaag gttgtatcat gatgcagcct tatgatattg aaaaaggtgc aggaactatg
    42721 aatcctaata cttttttaag atcattagga cctgagcctt ggcaagtatg ctatgtagag
    42781 ccttcaagaa gaccagcaga tggaagatat ggtgaaaacc caaacagact atatcaacat
    42841 catcaattcc aagttatact aaaaccttct ccagataata tacaagaatt atatttggaa
    42901 agtttaaaag agattggaat tgacccaagt gagcatgaca taagatttgt tgaagataac
    42961 tgggaagctg ctacagttgg tgcttgggga cttggatggg aagtttggct agatggtatg
    43021 gaaataactc aatttacgta ttttcaacaa gtaggtaata ttgaatgtga gcttgagaca
    43081 ggagaaatta catatggatt agaaagatta gctatgtaca ttcaagaagt tgatagtgtt
    43141 tatgatttaa aatggaatga taaaataact tatggtgaag tatttaataa agctgagtat
    43201 gaaaactcaa tgtacgcatt tgaattatgt gatgcagata tgttgtttaa tctgtttgat
    43261 atatatgaaa aagaagcatt aagacttatg gaaaatggcc ttgtaatacc ttcatatgac
    43321 tatgtgttaa aatgctctca tgcatttaat acattggatg caagaggtgc aataggagtt
    43381 agtcaaagag cttcatttat aggtagagtt agaaatatgg caaagactgt tgcagaaact
    43441 tttgtaaaac aaagagaaga aatgggattc cctcttttaa aggacggtga caaataatga
    43501 aaaattatct tttatttgaa atcggtgttg aagaaatgcc atcaagattt gtaggcagta
    43561 cactagaaca attaaaaaac aatcttagta aattatttga tgaaaataga atagcttttg
    43621 ataaaataaa tgcgtatggg acaccaagaa gacttacttt agtagtcgaa ggattgagtg
    43681 atagacaaaa tgatttagaa gaggaaataa agggtcctgc taagaaaatt gctgttgatg
    43741 cagaaaataa tttaacaaaa ccagcattag gatttataaa aagcaaagga ttaagcgaat
    43801 ctgacatata ttttaaacaa gttggaaaag aagaatatat atttgcgact ataaagcaag
    43861 atggaaaaga aacttcagaa gtactaaaag acatattacc agagactata aaatctgtag
    43921 tattcccaaa agctatgcgt tggggaggaa aaaatcttaa atttgcaaga cctataagat
    43981 ggatagttgc attgttaaat gataaagtat tagagtttga tttagaagga ataatatctt
    44041 ctaatgtaac aaaaggtcat agatttttag gtgaaagtac aatagaagtt gattctatag
    44101 aagattattt tgaaaaatta gaaaaaaatt atatagtttt agaccaaaac aagagaaaag
    44161 aaatgataag aaaacaatgt atagaagtgg caaattcttt aggtggagaa gttgaatttg
    44221 atgaagattt acttgaagag gtaacttact tagttgaata tccaactgct ttttatggag
    44281 aatttgatgt ggattatata aaactaccaa aagaagttgt tataactcct atgaaacaac
    44341 atcaaagata ttttccagta cttaaagaag gaaaactgtt acctaacttt atagctgtta
    44401 gaaatggtga ttcttacaga atagacaatg ttaaagctgg aaatgaaaaa gttttagaag
    44461 caagacttgc agatgcttta tttttctata gagaagatac taagagaaac ttagaaagct
    44521 acatagaaaa attaaaaact gttgtattcc aagtaaaact aggaactatt tacgacaaaa
    44581 ctttaagact tgaaaaatta agtgctgata tacttgatag tttaggttta gtagatgaaa
    44641 aggcagatac tataagggct gctaaattat ctaaggcaga tttagttact ggaatggtag
    44701 gagaatttga tgaattacaa ggttttatgg gaaaagaata cgcaaaagtt ggaggagaaa
    44761 atgatgttgt aagtgaagct atttttgaac attatcttcc aagatttgca ggagatatac
    44821 ttcctaagac taatgcagga gttgccttat caatagctga taagttggat tcaatagcag
    44881 gtttctttgc tataggaata caaccaacag gttctcaaga cccatatgct ttaagaagac
    44941 aagctttagg tatacttagt atacttatgg acagaaaagt tgctgtggat ataaaagtac
    45001 ttattgaaca tgctttagac aattattctg aattagattt taataaagaa gaagttgttg
    45061 agcaaataat gaatttcttc aatgagagag taaaaaatct atttaaagat ttaggaataa
    45121 gatatgatgt tgttgatgct gttttaagtt ctgatataaa tgatgtttct gatatgcata
    45181 tgagagcttt agagcttaat aattggctag aaaaagatga attagtggag atgcttactg
    45241 catttaatag agtttctact ctagctcaaa aagcagaaag tgataaaatt gatgaatctt
    45301 tattaaaaga agatgctgaa attaaacttt acaatgaatt taaacaagta aaggtaaaag
    45361 ttaatgaatt attaaaggat aagaaatatg gtattgcttt agatgaattt gcatctttaa
    45421 gaccagtagt tgataattta tttgatacag ttatggttat ggataatgat gaagctatta
    45481 agaacaatag acttgcttta cttaaacaag tatatgatac tatgctagga atttgtgatt
    45541 tatctaaaat tgtttataaa taaaatttaa actatttata agaaataagg ctgtctcaaa
    45601 atagagatag tcttattttt cattgttgag atgatgaaaa tatatttgat ttcatagaaa
    45661 aagagtgtct cataaactaa aaagttcatt ttgagacacc catgcttttt gtaagtaaat
    45721 ataataaatt atattttata cttatcagtt attttgttta aaatattatc atttgaattt
    45781 acccattcat aaaaattgcc tgatgatgcg tccgatattt tataaagcat ttcaggaata
    45841 tcactagctt taaaatctcc aagtgatttt cccaatctag tcttacctat ttcaaaactg
    45901 cctccaacaa ataattcaaa tgcatctgta gatataccat caacattctt ctttttacca
    45961 cataaaccta tgctaccaac ttgatgaaca ccacatgaat ttaaacatcc tgatatatat
    46021 aacttaggtg ctttatttaa aatttcttta ttattttgta atctaaaata atctatgatt
    46081 tcatgtaaca ttttctgact attttgtatg cccatttgac aaattggaac acctatgcaa
    46141 gatatacttt gttctaattg agaattacaa ctaatagttt tacttatttc taatattctt
    46201 tttgcttcat ttccatttaa atttaaaata tataatccct ctgtcattcc tagtctaatc
    46261 ataggatttt caatattatc taattcttta agtaaagtac ttaaatcctt tgttaacaaa
    46321 atacctccaa ctggatgtat atatacagtg tataaaccat cctgtttttg aggtattagt
    46381 ctagaatcct gtatatctat tttaacccca ggtttagagt aatctatttc ttcaatattg
    46441 agttcaagac taccattttc tttttctaat ttatagtatt ctttaaattt ttctaagaat
    46501 aatttttcac caagtgattc gaccatatat ctaaccctag ctctgttttt attttcataa
    46561 tttccataat ccataaagaa tttaatcata ccttcaacac aatataaggc atctttcggt
    46621 tttataagtt catcaagttc taaagcaact ttgggttgtt tacctagacc tccacctaag
    46681 aaaactctga aatatggttc atcatttttc aaagtagcaa caaatcctaa gtcttgtata
    46741 ctacaatgag cagtatctgt gtaacaatta gaataagaaa cttttaattt tctaggtaaa
    46801 tggtatgtag tagcattttt gataaaatat ttatcagtag caactgcata tggagtaaca
    46861 tcaaagactt cactagggtc aactccagat aatggagata atccgacatt tctaggataa
    46921 ttaccaccac cacctctagt gaatatatca ttttttattc cttcttccat gatatcaaca
    46981 attgaattta tagataaatc gtgtaattgt atagcttgtc tagttgttaa gtgtatctta
    47041 tctaagttgt gtttaatagc tatttcatag attttattaa gttgatactg agataaaact
    47101 ccagcagata ctctaagtct tatcataaaa gattttttgt ctctttgagc ataaacacca
    47161 tatccaccag aaaatccctt atattgcatt ttatttattt ctccatttaa aaattttgaa
    47221 gaatattctt taaaattagg tagttcattt aacaatatct ttttaaaatt atccattatt
    47281 tcacctcatt gttagtcttg tataaaaata tatctattgt ataatagata tatatattat
    47341 tatctagtca ggataaaaat ataacagaat aattctatta aaagtaagaa taataaggac
    47401 atagtgagta ttttgttttt attttacaaa ttaagtataa tatttttgtt ttataaaata
    47461 taattaagat aaatagataa ttttgtaatt ttaatatata atatttagta gagtgtggga
    47521 gtagcactta atatttgaag gaggagtttt tgtatatggg agaaataata aatgcattag
    47581 atagagccct agacataatt ttgctgttat atcatgaaaa gagagaaatg ggaataactg
    47641 aaataagcaa ggctatggga gtatataaaa gtactgtaca tagaactttg gttactcttg
    47701 aaaacaaagg ttttgttata caaaatgcag agaatggtaa gtattggcta ggaataaatt
    47761 tatatgcaat aggtatggtt gtgggagaaa aaatgtcatt gactgaaata gtaaaaccat
    47821 atacgaaaaa attaaatcaa gaatttaacg aagtagttaa tgtatctata ttagaagaaa
    47881 gagctcaaga ttctccaaga agtatcataa tacataaaga gtatggttca aatcaattac
    47941 tttcagtaaa tccatccgtt ggttcaagta gtgaatgtta ttgttcagca gttggtaaat
    48001 gtcttatggc atttaatgat tctatagatt ttgaaaaata tagaaaaaca ccaatacaca
    48061 agtatacaga acatacaatt gataattggg atgatatgat gcttttttta gcaaaaataa
    48121 aagaacaagg atatgctata gatgatgaag aactagaaca tggactaact tgtattggag
    48181 cacctatctt agataaaaat aataaagcta ttgcagccat aagtttgtca ggtccaacta
    48241 taagaatgag agaaggggac tttgagtata agattaaaag agttatagaa actgcaaaaa
    48301 gtatatctga actttttaga tgatttttaa tctcgataat aaaaataggc tatatttaaa
    48361 ttgttatttg taaatatcag tttaaatgta gcctattttt ctttaatcta aaattaaata
    48421 ttcttaaaat ttaaaatgtt attgaaatat ataagcgaat gtgttatctt ttaattaagc
    48481 aataaaaata gaacaaggtt ctatattata gaacaaaatt gatgcatata tagtatttat
    48541 acaaaatata aatattatat atttaaaatt aagtaaaaat attacataaa ggggagattt
    48601 gagaaatgga aaaagtcaag ttgagtgttg aaagatgtaa gggatgctat ctttgtattg
    48661 aagcttgtaa aaaagatgca ataagtcttt ctgactacac aaataaaaaa ggctttgtaa
    48721 ctattaaagt cgatgaagaa aaatgtattc aatgtggcgc ttgttataca atgtgccctg
    48781 atttagtatt tgagatatta taaattattt ttattagagg ggagaaaagg tatgcaaagt
    48841 ttattaatta aaggaaatga agctttagca gaaggtgcta tacgtggtgg ctgcagattt
    48901 tatgcaggtt atccaataac gccacaatca gaaatattag aaatgatggc atggagacaa
    48961 aaagaagttg gaggagtatt tatccaaggt gaatcagaaa tagccagtgt aaatatggtt
    49021 atgggagcaa gtgcacttgg agcaagagct atgacttctt catctggtcc aggattttca
    49081 ttaaaacaag aaggtattgc atattgggta tcagctggtt tgccagcagt tgtagcatgt
    49141 gtgcaaagat atggtatagg tgatggattt ataggagctg gtcaagataa ttattggcaa
    49201 gcagtaaaag gcggtggaaa tggagattat catttaatcg tgttagcacc taatagtgtt
    49261 caagaaaata tggatatggc atatgaatct tttgaattgg ctgaaaaata tatgcatcca
    49321 gtacttatac tttctgatgc tacaataggt caaatggtag aaccttgtat agcaccacca
    49381 atgaaagaat acgatatgga taaagctcca tgggctgcaa aaggaacacc tgtaggagaa
    49441 aaaaataaag taataactga tataacatat tttgaagacc aagatacatg gcaaaaaggg
    49501 tatatagaaa aaatgagaaa agttcaagaa gatgaacaaa gatgggaaga gatagaaata
    49561 gaagatgctg aagtggtatt tgtggcatac ggtatttcat caagaatagc agaaggtgca
    49621 gttagagatg ctagagcaaa aggaatgaaa ttaggtctaa taagaccaaa aacattgtgg
    49681 ccttacccaa gaaaagcatt taagaaatta agtgattcat tgaagggact tgtaacagtt
    49741 gaaatgaaca tgatggcaca gatgaaggaa gatgtaatta tagcatgtga taatagattt
    49801 ccagtatatt cagtagcaac agctcaatat atggcagaag taaatcaatt aatagatttt
    49861 gctcaacaga ttatagatgg aaaagctaag caagaggagg tattttaatt atggctaaaa
    49921 aagcaccagc aataatgatg tgtgaaaata tgttctgtgg aggctgtgga cactctatag
    49981 tcgatagaat tattggagaa gtattaacag aaatggatat atgtcaagat acaattatat
    50041 gttcagatat aggttgtaat catatgtttc aatttagtat gaatgtagat gttgtcgttc
    50101 cacctcatgg taaaatggga gcagcaatgg cagctatgaa aagagttaga ccagataaat
    50161 atatatttac aatagctgga gatggtggag cttatgcaat agggcttgga gaaactatgt
    50221 cagctgctac tagaaatgat aatgtaatat ttcttgttat gaacaataat atgtttgcaa
    50281 tgacaggagg gcaaatggct ccaactacta tagaaggtca atatacagct tctactccag
    50341 cgggtagaaa ttacgcagat catggttcaa catttgatgt tgtaaaagtt atgggaaatt
    50401 tagatatagc atatctagca agaggaacaa taactaaccc taaagaaata aatacaacaa
    50461 aaagatacat caaaaaagct ctagaaaaac aaaaagaaaa gaaaggtttt tgcttaatag
    50521 aagtgcttgt accatgtcca acaaactggg gattaaagcc agtagatgct atgaagagaa
    50581 ttgaaaatga acttgttcca aagtatccac ttggagaatt tattgattag ggaggaaata
    50641 atatgttgaa tgaaattata tgtgctggtt ttggaggaca gggagttctt acagcaggaa
    50701 aaattataat gtatgttgca tataaggcag aaaagaaaat tacatggttc ccatcttatg
    50761 gaaatgagat gaggggagga agtgcaaatt gcaatgttgt tgtaagtgat gaaaaaattg
    50821 caaatccttg tgtaaaaaat ccagatatac tattgggaat gaacagcgta tcagtagata
    50881 agtatgaaag tttaatgaaa aaaaatggtt atatgtttgt caatgaatca ctagtaagta
    50941 aggatagaaa gtacagagaa gacattaatg ttataaaagt acctgttact gaaatagcac
    51001 aaaatttagg caatgaaaaa gctgctaaca tttgcatgtt aggtgctatg gtaaagagtt
    51061 tggatttatt tacaaaagaa gaatttgaaa gtggtatgtg tgaatacttt gaaaatcaag
    51121 gcaaaggtaa attcaattct aaaaatgtag aagcatttga agctggatac gactacgaat
    51181 gaatatttta aatatttaaa ataattattg aaatacttat ttttatatgt tagtatttaa
    51241 ttaataaaat atagaacgtc gttctggaca atgggacgaa gtctttaggt cggaatacaa
    51301 gaacggcgtt ctgtataata gaactagggg gtgttaaatt gaaagtttta gtaataaatc
    51361 ctggtggtac atccacaaaa atttctgttt tccaagatga aaatgaaatt ttgaaaaaaa
    51421 atataaccca tacaagagaa gatttaaaaa atttttctaa agtatttgat gaatatgatt
    51481 atagaaaaca actgatagta gatattttaa gtagtgagaa tcatagtata aattcttttg
    51541 acgcagtagt tggtcgtggt ggacttatga aggctataaa aggtggtacc tatactgtga
    51601 gtgaagaaat gattgaggat atgagaaatg aaataaatgg ggaacatgca tcaaatttag
    51661 gtgcattatt agctaaaact atagctgatg aaataggagt acaatcattt gtagtagacc
    51721 cagtttctgt agatgaattt gatgatgtat cgaggattac aggaatatca gacatagaaa
    51781 aagcaagttg gttacatgca ttaaaccata aagctgtttc aagaaaaatt gcacaaaaac
    51841 tagggggaaa atacgaggat tataacttta ttgtagctca tttaggttca ggaatatcga
    51901 ttgtagcaca taaacaaggc aaaatgattg atggaagtgg tggtaggtct gatggtccat
    51961 tttctccaga aaggagtggt ggattacttg catatcctct tatagaattg tgttactccg
    52021 gaaaatatac tcatgatgaa atggtcgcaa aagtaagttc gattggggga atgtacgatt
    52081 atttaggaac aaaggacatg atagaaattg aaaacagaat caaggagggt gatgaaaagg
    52141 caaaacttat aatggatgca tttatttatc aagtagcaaa agaaatttct atgtatggag
    52201 cttcactttc agggaatgta gatagagtta tcttgacagg agggatatct cactcagaaa
    52261 tggtagtcaa aggagtagca gaaaaagttt catatctagc tccaatagag atagtaccag
    52321 gagagatgga aatggaggca cttgctctag gggcacttag agtacttaat ggagaagaaa
    52381 tagcaaaaac ttattaatat ttaggagaaa gagtatgtta aaagatttta ataatttatt
    52441 agaagaggct aaaaagcttg aacctgttat tgtaagtgtt gctgttgcag aagacaaaga
    52501 agtattaaag gctgtgaaat cagcaacaga tttaaacatt ataaagccaa ttcttgtagg
    52561 agacaaggat aaaatagaaa gtatatcaag agaaattgga ttaaaaggtc atgaaataat
    52621 aggttgtcaa aatcccgatg aatcagttaa aaaagctgta gagatagtta aaaataagga
    52681 agctaaagtt cttatgaagg gattagtaaa tacaagtgtc tatatgagag ctatattaaa
    52741 taaagaaacg ggtcttagaa caggaagatt attaagtttg cttgcagtat atgaattacc
    52801 tcaatatcat aaacttcttt attgtactga tagtggaata aatgtatcgc caaacttaaa
    52861 tcaaaaaaaa gatataatga caaatgcact tcttactatg aaaagtatag gcatggaaaa
    52921 tcctaaaact gcgatactta ctgcaaatga aatggtagac cctaagatac aatctacagt
    52981 ggatgccaac ctacttgtag agatggaaaa atcgggagaa ataccaaagt gtatagtgga
    53041 aggtccaata gcatttgatg tagcatttga tgcacatgca gcagaacata aaggtattaa
    53101 aagtaatatt gctggtgatg ttgatttatt agtatttcca aatatagaag cagggaatat
    53161 attaggaaag tcatggctta gatttaataa agctaagtgg gcaggtatag ttttaggagc
    53221 aacaaatccg ataattttag gttcacgttc tgacacagct gaaataaaaa taaattcaat
    53281 agcattagcg tgcctagcat cacaataaga aattattatt aaggaggaaa aagtattatg
    53341 aaatatgcaa aaagaatgga tatggtaaaa gcttcagcta taagagattc tcaaaagaaa
    53401 atagctcaga aggtagcaag tggtggaact gttatatctt ttgctgcagg gcttcctgac
    53461 ccaaatttat ttccaataga agagataagt gaaataactc aaaaagtact taatgaaagg
    53521 ggaaaatttg ccctagcata tggacctact aaaggtgaag atgagttgtt agatctatta
    53581 gtaaaaagaa tgaaagaaga agaaaatatt gatacaaaag cagaaaatat tataataact
    53641 actgggtcac aacaagggat agcattaagt gctatgattt tattagaaaa gggagatgta
    53701 gtagttacag aaaatccaag ttacttaggt gcaataaatg catttagacc ttatgaatgt
    53761 gattttttag gagtagatac agatgaagaa ggaatggtaa cagatgattt agacaaatta
    53821 ttatctgaaa attctaacat aaaggtgata tatgtaatac caacatttca aaaccctaca
    53881 ggaaaaacat ggtctttaca aagaagaaaa gattttatgg aagtcgtgaa taaatatgat
    53941 gtcatagtaa tagaggataa tccttatgga gaaataagat ttacaccaga acctctacct
    54001 acacttaaat ctcttgatac taaagataaa gtattgtacc ttgggtcatt ttcaaaggta
    54061 ttatgtccag gatttagagt tgcttggatg tgtggtaatg tagacatcat agataaggca
    54121 gaacttttaa aacaaggtat agatttacag tcaaatcaat tttcgcaagt acaagtaata
    54181 gaatttttaa agaaatacaa cttaaatgaa catatagaaa aaataagagc agagtataag
    54241 aaaagatgtc tgctaatgtt agatgctatg aagaaacatt tcccagagga agctaaatat
    54301 acagaaccag atggaggaat gtttatatgg gttgaacttc ctgaaagtat aaatgtagat
    54361 gatttattag ataaagccat tgatgctgga gttgcttatg tttctggaga gtccttcttt
    54421 gcaaataatg gtccaaaaaa tactatgaga cttaactata caacaatgtc agaagaacaa
    54481 atcgttaaag gtgttgagat acttgcagaa gttataaaaa aagaactcaa ttatgcttgt
    54541 gaataaatat aaaaagtgag ggtttaaata tgggagaaaa agaaaacggt ataaagtttg
    54601 gaaagttaat gtttcaactt gttattatag caatatcttg ggaattagtt tatattatac
    54661 catttatcca atatacatta tatgatccaa tcctaaaagc attagaatgt acaaacactc
    54721 aactaggttt tctacttaca atttatgggt taggtaatat atttggagct cctataggag
    54781 gctggttagc agatagattt gattatagaa agataatttt aggttctgta tttcttaatg
    54841 gagtagtatc atttttattt gcatttaata tgaactatcc atttgcagtt gctacttggg
    54901 taggttgtgc aatcacatca ttagttatga actatccttc tatggtaaaa atacttagag
    54961 ttataggaaa agataatcaa ggtaaggttt atggatttaa tgaagctatg gttggagttt
    55021 ctggagtagt aatgggagca atattcttat atatttatac atgctttgca actcctacat
    55081 tgggtatgag atgggtaatg atatctttag gaatattatc aatagcaatg tgtccagtat
    55141 tatggtttgt aattaaagat gtagatatta aagaagaaag agaagaagca ggtgaaaaaa
    55201 tgtctgctgg tgattttata acagtactaa aatcaccaaa tacttggcta gtaggaataa
    55261 gtatattctg tgtatattca ttcacagtta caatgtctta ttttacacca tatataacat
    55321 ctgtacttgg aggttctgta gcattatctg gtgcattagc aataataaga cagcatggtc
    55381 taaaattatt tggagcacca tttggaggat attgtgcaga caaattaaaa tctcctacaa
    55441 aagtactttt accaatatat gtatttggta tagcagtaat agttttattc ctagtattac
    55501 ctgcatcaac tccaatgact atattcatag ctttaacatt tgtagtaggt atattaggtt
    55561 acatgggaaa aggtatatat tatgcagtac aagatgaagt aaaagttcca gttaaatatt
    55621 cagcaactac tataggtata gctgcagctt taggattttc accagatgta ttccaatttg
    55681 cattaatagg acactggata gatacttatg gcaataaagg ttacacatat acatttatat
    55741 tccaaatagc aattctagtt attggaatac tatcttgttt atatatatta aaggttaaaa
    55801 agagaagagc aagtaaatta gaagcttaaa tattcaattt ggtaattaag tatatataaa
    55861 tacaatactt attttgatga atgtgattgt ataaataaca taaaaaatat caatagatta
    55921 agttttataa taatgtttag agaataaagt ataaaaatca aaatgttatc aagttttcta
    55981 ggttttaaca taaagttacc cctgaaggtg taaaattgag tggcaattat tatctatttt
    56041 cttacctaca taaacctatt taatttaaat tctaagagat tttcaaaata gatggtagtt
    56101 gtactcaatt ttaaacctca taataaatac gaaaatttaa aatctaacta gatactattt
    56161 aattatatat taataaatat atattgtact atttatataa taataatttc aggcttttca
    56221 ttatatgtat tccaatttat attgtttgat tattattcaa acacacgtcg ttaaaaattt
    56281 tataaattta tgtttagata tgattaatac tcttgatttt agaaataaat atttgtagat
    56341 tgtagattaa agataatata aataaataga aggtagattt tttagatata ttaatataaa
    56401 gattatataa aatattgaaa tatagctgtt taaattattg tctataaata taaaatgaat
    56461 ggtggtgagt taagtgaccg atttagttaa caccattaat ggaattgttt ggtcacccgt
    56521 attagttgca atgtgtttgt gtataggatt atatttttca ttgaaattaa aattttttca
    56581 aataagagat gtaaaagaaa tgtttactct cttgcttgag ggagatagtt ctcatgttgg
    56641 tatatcatca tttcaaggtt ttgctacatc tatggctgga agaataggga ctggaaatat
    56701 agctggtgta gctgtagcaa tagcaatggg aggtccagga gcattagtat ggatgtgtat
    56761 aatggctaca ttaggttcag caactgcatt tgtagaatct actttagctc aaatatataa
    56821 agatgagcat gatgggcagt atagaggagg acctccatat tattttgaaa aaggtcttgg
    56881 gtggaaaaag tgttcagttt tattttcaat agttgcaata ataagttata cattttttat
    56941 gcctggtaca caatcaaaca ctttatatct agcaatgaat caagcatttg gattttcaaa
    57001 aatgacaata gcagtagcat cttctattgc attagcgata ataatttttg gaggaattaa
    57061 aaggattggt ttatttgctg aaaaagtagt acctataatg ggtggagcat accttattat
    57121 gacattagta ataattataa caaatattag tttagttcca gaggctattg taacaatgtt
    57181 taaatctgca tttggatttg gttcagtttt tggaggaatg ctaggttcag caatatcttg
    57241 gggagtaaaa agaggtatat actcaaatga agcaggagaa ggttctggga cttatggtgc
    57301 ggcagcagca gaagtaagtc atccagccaa acaaggatta gttcaagcat tttctgttta
    57361 tgtagacaca ttattaatat gtatggctac aggtattatg attgtaataa caggaatgta
    57421 taatgttaca ggtgtttgca cagcactacc aggtgtagaa ccgggaccac aatatgtaca
    57481 gagtgcaata aatacattac atccacaatt aggtgatatt tttatagcaa tagttttatt
    57541 attatttaca tttacaactt tattagcctt ctcatatatg atggagacta atgtatctta
    57601 cttagtaact agaattaaag cagaaaaaca tgaaaagtac ataattaatg tctgtagatt
    57661 tatgcttata gcatgtgcag caatgtgttg ttttatagaa ccacttacaa gctgggcatt
    57721 aggtgatata tgtataggtg tattaacata tataaattta attgccatct ttacactaaa
    57781 aaaaccagct ataaaggcat attatgatta tataagacag aagaagatgg gaataaaaag
    57841 gagtgataga acttttaacc ctatagagtt agatataaga aatgctgagt tttgggaaaa
    57901 tagatactta aaagaggata agcatgagtt aaattaaagt tataataaaa tataaaaatt
    57961 actaaataaa tttagtggga ggtaagattg gtatgaaaga aagaataata caagtggctg
    58021 attcaaagaa agaaaaaatt ctagggctat gtcaatcttt atatgatgaa ccagaaattg
    58081 ctttgcaaga atataaatct gctaaaaaaa tatcagaatt tctaagagaa gagggttttg
    58141 atgttgagga aaacttggca ggtatggcta cagcttttaa agcaaccaag aaaaatggag
    58201 atggtcctaa aatagcattt atagcagaat atgatgctct tccaggcaat ggacatgcat
    58261 gtggacatca tttaattgca tctatgggag ttggagctgg aatagctctt tcaagtatac
    58321 tggatactta caagggagaa gtaagtataa ttggaacacc tgctgaagaa actggagatg
    58381 gaaagcctta cttgatagaa catggtgtat ttgatggcta tgatgcagca atgatgatac
    58441 atccaaattc aaaaacttgt gtaacacctg aaattatagc aataggaggt ctagatttca
    58501 tatttactgg aaaagcatct catgcaggag ctaagccata taacggaata aatgctttag
    58561 atgcagtagt actactatat aataatataa atgcactaag acaacaatta gtagatggaa
    58621 cgagaataca tggtattata ttagaagcag gaactgctgc aaatgtaata cctgatatgg
    58681 gaaaagtaag attagagata agggcaaaag aacaaaatta ctttgatgaa gtagtagaaa
    58741 aagtgaaaaa ttgtgctaga ggtgctgcaa tagccactgg atgtgaactt gaattttatc
    58801 actttgaacc aacttgccaa ggattaaatg aaaataaagt tttggtagac atatttacaa
    58861 agattatgga ggaatttgga atatatgaag atgaacaagt aatgttaggt tcaacagata
    58921 tgggaaattt aagtcaaata atgccatgta tacatccttt gatgaagttt tctgaaaatg
    58981 gagaagaact acatacaaaa gaatttttgg aggcttctat aaattcatat gcaaaagata
    59041 gagtaattga tggtataaaa atattagctt taacaggatt taatttattt gaaaatccag
    59101 aattattgaa aaaaatgaag gaagagtaga acttagacta tttttaatta tactctttaa
    59161 aataagaaga gtgtctcaaa atgaactttt tagtttatga gacactcttt tttgtatgaa
    59221 attaaatata tttttattat tttaaacatg aaaaagaggc ttatctgttt ttatatgacc
    59281 ttttttaact tgtagcacaa aatatcatga atgattacaa ttcgaatgat aaaatcgatt
    59341 ataagatata taggaggggt acatatggaa actttagaag taagaaatat tgaattgagt
    59401 ggtacaaatt atgagatagg atacagacta ggagaattgg ttgctaatat gccagagata
    59461 atagaaggtc agattaataa atctaatctg gtaagcaaaa aagaagaaaa agagatgata
    59521 gagttgtttg ataaatattg tccaggtctt aatgaagaat tgcaaggttt tgctgatgca
    59581 attcaagtga attgtaatca aattttatat tatactatga cttatcttaa accaggttgt
    59641 agtcaagttg cattagcacc agaattaact gaaaatgggc atgtactatt tgcacgtaat
    59701 ttcgatttct cacacaatat ggaggatttt gttttatgta aaactaaggt aaatggaaaa
    59761 tatgcacata taggtactac cattatgcaa tttggaagag gtgaaggtat gaatgaatgt
    59821 ggcttaggtg taagtcagtc atcatgtggt attcctgtag gcaattcaga tggtttaaga
    59881 aaaccagcaa ttgtaggttt acagttttgg gctgttatta gatatttgtt agaaaattgc
    59941 aaagatgtag atgaagcatt agaatattta aaagatatgc caattgcata caatattaat
    60001 ttattgcttg cagataagtc tggaaacatt gcacttgtgg agactttaga tgggaaaaaa
    60061 gaagtaaata taatcaacag tttagaaagt aaaagagaat attttttaca ctctactaat
    60121 catacacata ttgataaact tcataaatta gatccacaat caatgaaaaa ttctattcat
    60181 agatataaat taattaaaga atatataaat aagtctaaaa aaattggaga gaaagagtta
    60241 atgaatttat tgtcttcaaa atatccaaat ggcttatctt gtaattacta taatgatttt
    60301 tttggaactt taaaaagtat agttatggat ttaaatatag gaaagtttaa tattttatgg
    60361 ggtggactag agaataaatg ggagagctac tatttaaaaa atgacattaa atctataaca
    60421 cagaggataa acataaatat tgaaaaagca ccatctgact tttttgattt cattgaataa
    60481 aaaatgtatt taattattct aaataattag gacatataaa tcaactaaaa gtttttattt
    60541 atcgtattga attagttata atattttagt atagtaaaaa atagtgctat tttacatatt
    60601 taaagagttt aagtagaaga gaggtgccat attggattac ttaaagtatt ttgaaaaagc
    60661 tgtaatgtat attgagaata atttacatga aaatataact gtaaatgatg tggcaaaaga
    60721 aactggatat tcttattatc atttaacacg cttatttaag tctatgtttg gtgaaagtgt
    60781 aggaagttat ataaaaaaaa gacgattagt aagtagtaca aaagaacttt tatatagtga
    60841 taaaaaagtg atagatatag ctattggtag tggatttgaa tcttcagaag catttagcag
    60901 agcatttaaa tctatataca aggttagccc tatagaatat agaaaaaata gaattgatgt
    60961 atttgttggt aaaaagaaaa aattagagtt agattttatg agacatcttg ttggaaatat
    61021 aacgattaaa ccagttataa aagaaataga aaaaataaag gtaataggta taaaggataa
    61081 agtaatttta gaaaataatt cattacctga cttgtgggag aaattcagaa aagtacatca
    61141 tattgtccca aatactttgc catcaaggag agtttttgga atctgtgaag caatctctga
    61201 aatacaccta gttagtgaaa gtatggagtt taatgagata ataggacttg aagttaatag
    61261 ttatgatgtg attccaaatt catttgtttc aaaaactatt aaggaaggaa agtatgctat
    61321 atttactcat actggtagct tagataattt agacaaaact tatgaatata tatggggtac
    61381 ttggttttta agttcaaaag aagaacttga tataagagat gattttgaag tatatgatga
    61441 acgattttta ggtcctagta atgtgaactc acaaattgat atttatatac ctataaaata
    61501 atctataaga aaactaaaag ataatttgat taagaaatag atgttcgtaa aagag
//
LOCUS       DAYAPA010000005        60367 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.005, whole genome shotgun sequence.
ACCESSION   DAYAPA010000005 DAYAPA010000000
VERSION     DAYAPA010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 60367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60367
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.005"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(208..1785)
                     /locus_tag="ACGI59_000259"
     CDS             complement(208..1785)
                     /locus_tag="ACGI59_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp5"
                     /protein_id="HFG9738073.1"
                     /translation="MKAPKTILTILTIALTLSSISIIPSYALTEEKLIGNGRYETAVK
                     ISQKAYSSSPNVVLVNDNSLADALSATPFAKAKGAPILLTESDKLDDRTEKEIKRLGA
                     KDIYLIGGTAVLNKDIENKLKSNGLNVERINGKDRYETSLILANKLKDIKDIKEVAVV
                     NGEKGLSDAVSVGAPAAQNKMPIILSSPKDGVEAFDKFIRDEKVIKAYVIGGTNSVSR
                     AVEKSLPNAERVSGKDRNETNAKVIEKFYTDTNLSNLYVTKDGSKNENQLIDSLAVGV
                     LAAKNESPVVLVGNKLNTKQRDILSTKKLNTITQVGGNGNEEAFDEIKSLQEKTVFEA
                     KTVEELTDMINIASPNDIINFKPKENTVNEAFRMVTNKPITVNIKGDCSKTLTVDMPN
                     GEVNNYATLVNVIVRNIGEGGFNNHDTITILSVRDKNGRVIENTRNSDIDTLMILASA
                     NDTKLINDGYIGKLIDNSSNSDITNNGTIDKKVNQVEDLEAKVDSIEKAIDSISQKVN
                     KIQDILDKLGFLKKFLS"
     gene            complement(2082..4493)
                     /locus_tag="ACGI59_000260"
     CDS             complement(2082..4493)
                     /locus_tag="ACGI59_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HFG9738074.1"
                     /translation="MRKYKLKKLSKLLVLLTVCFLIVSTIPVSAEKNKILTGVETAEY
                     SESYLQYLEDVKNGDIAKYNGVIPTPYEMEGTTLKTNVRSSLPASYKSSVSYDPRKLD
                     LTTPAKDQGKLNTCWAFSGMSTLEAYLKLKGYGTYDLSEEHFRWWSTGGIHGWNLTDM
                     TGSSNVTAIGYLTAWAGPKLEKDIPYNFKSEDEGATRPQNMDTAPTQFNVTDVVRISK
                     DKTSVKNAIMQYGAVTSGYAHYSAYLSDDENSYNCNDKSEPLNHSVSIVGWDDNYSKD
                     KFKPSVRPESNGAWLVKSSWGEFNSEKGFFWISYEDKTLLKDTDNYAMKSVSKPDSDK
                     KMYQLEYAGLSKIMSNKVTAANVFDFSRDSEKLDSVMFETDSVGAKYEVYYAPVVNGV
                     PQNNSMTKLASGTVSYSGYINVPTNSYSLPKGKGAIVVVIDNTANPNREKSTLAYETD
                     IDGYYLYEAKANLGESYILQNNKFEDINTYSEFSPCNFVIKAITKTSSGQATSGESLT
                     GADRYETAVKVSQKGWTSSQNAVLVNGDAIVDALTATPFTAAIDSPILLTGKDNLDSK
                     TKAELQRLGTKKVYLIGGENSLSKNVQTQLSNMGISVERISGSDRYKTSISLAQKLNS
                     IKSVSQVAVANGVNGLADAISVGAAAADNNMPIILTNEKSELQGADEFLNSSKITKSY
                     IIGGTATLSSNLESKLSNPTRLAGSNRNETNAKIIDKFYPSSDLKYAFVVKDGSKSQG
                     DLIDGLAVGALGAKTDSPVVLVGNKLDESQKNVLKSKKIETPIRVGGNGNENAFNELN
                     TLLGK"
     gene            4668..5084
                     /locus_tag="ACGI59_000261"
     CDS             4668..5084
                     /locus_tag="ACGI59_000261"
                     /inference="COORDINATES: protein motif:HMM:NF016060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HFG9738075.1"
                     /translation="MEENFLILKKHLETILYLFFGALTVLINIITYLFFTRIINFTFL
                     LSNSLSWSIAVLFSYITNKFFVFKSEKTEFLFLLKEFVSFLSCRLFSGFIEILLMYLM
                     IDLFSLNDILVKIGTNIIVIILNFVFSKVVVFKHRN"
     gene            complement(5149..7266)
                     /locus_tag="ACGI59_000262"
     CDS             complement(5149..7266)
                     /locus_tag="ACGI59_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HFG9738076.1"
                     /translation="MQISKKIISLLTAAFLSIVLPSNVVNASNKDSLIGSGRWETAIK
                     ISQNGWLRSDNAVLVNDNSIADALSATPFAKLKDAPILLSGSKELDFRTKSELKRLGV
                     KNVFLVGGSNALGVNVENELKSEKITVERIFGNDRYETSLKLAEKLNNEKDISDIIVV
                     NGEKGLADAVSVGSIAAQNYMPIILSDSNLGVEVANKFIKAQNIKNSYIIGGLNSISN
                     EIEKNLPNSKRISGNNRNETNANVIKEFYKETDLRNAYITKDGMKKQSDLIDSLSVGV
                     LAAKNNSPVVLVGNKIDSIQKDVLNTKIFSTVTQVGGIGNENVYKDILDMQEETKYTV
                     ETIEELDSALKKVDANDIIKFRPDKKISESFKLETEKAIKVDLYGEYSQSITINMPNG
                     ELNNTGDISKTLILKDIKDGTLTNSGYINQMDISDDNGCKIENENNGQIWIVTIFSSA
                     KDVNIRNDGTITKIDNDSSSTNIRNSGKIDTVSGNKEPGISGNKPNTNNTDKDDDSDT
                     AAGLSPKISACSPLENNQIMLTVPQKPKNSNYKIYYRVLSYKPSAMRVGDRININDWD
                     LVEDTEPLKVNAKNGSYIEVVEIDYTRKKVSRWGRAGATDDGFKIVANGIKSTVSVDE
                     NSKVTISVTGYNGNKVYYRIKTTKPEAMNVGDEIDSSWKSTSNSDIILEGNKVNGLYV
                     ELASGENNKVEKWGATDKIEIVS"
     gene            complement(7297..8004)
                     /locus_tag="ACGI59_000263"
     CDS             complement(7297..8004)
                     /locus_tag="ACGI59_000263"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HFG9738077.1"
                     /translation="MVDATLFFTPHQDDETLSMGSAIIEHVEKSDTHVILCTDGSKSI
                     IRKVLDDGGSCSYHIKDVHKYSLSESEFSKDRDEEFKDSCEAMGVKESNIHIEDNRAH
                     DGELSKEKAKEIILKYLEEYPEAKVKTVTPFKASGIHEDHRALGEAALELYREGKIKD
                     LRFYVEPYDYKDFKKVNPNVEVWKVLPSQEEKLLSAMNAYKKWNPESGHYAIGYHSVK
                     SHFDDLELNKTQYVHAP"
     gene            complement(8111..9982)
                     /locus_tag="ACGI59_000264"
     CDS             complement(8111..9982)
                     /locus_tag="ACGI59_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp2"
                     /protein_id="HFG9738078.1"
                     /translation="MNKKNLSVIMAAAMISTSVAPVFAAETTQVKKETITKKEATELV
                     SKVRGLMSQKYTGGSQVGQPIYEIKVGETLSKLKIITNIDELEKLVNALGENKELIVT
                     ITDKGHITNSANEVVAEATEKYENSADLSAEANSITEKAKTETNGIYKVADVKASYDS
                     AKDKLVITLRDKTETVTSKTIYVGIGDEKVDLTANPVDSTGTNLDPSAEGFRVNKIDK
                     LGVAGAKNIDDVQLAEITIKNSDLNTVSPQDLYDGYRLTVKGNMVANGTSKSISDISA
                     KDSETGKYKFTIKYTDASGKATELTVESTNEKDLKDAKAALEGNSKVKLIAGDDRYAT
                     AVAIAKQTKYTDNIVIVNSNKLVDGLAATPLAQSKKAPILLASDNEIPKVTLDYIKDI
                     IKKSPSAKIYIVGGESAVSNTAKKQLESVTKNVERLAGDDRHMTSVAVAKAMGSFKDA
                     FVVGAKGEADAMSIAAKAAELKAPIIVNGWNDLSADAIKLMDGKEIGIVGGSNNVSSQ
                     IENQLADIDKDRKVQRVEGETRHDTNAKVIETYYGKLDKLYIAKDGYGNNGMLVDALA
                     AGPLAAGKGPILLAKTDITDSQKNALSKKLNLGAEVTQIGNGVELTVIQKIAKILGW"
     gene            complement(10326..12671)
                     /gene="secA"
                     /locus_tag="ACGI59_000265"
     CDS             complement(10326..12671)
                     /gene="secA"
                     /locus_tag="ACGI59_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFG9738079.1"
                     /translation="MSVLDTILDKADEQEIRRLNVIVDKIESLEKDIENMSDDELKKM
                     TDLFRDRLKKGETLDSLLPEAFAVAREASKRVLGMRQYRVQLIGGIVIHQGKIAEMKT
                     GEGKTLVEVAPVYLNALTGKGVHVVTVNDYLAQRDKELMQPVYEFLGMSVGVIIADLI
                     PEVRKVQYNCDITYGTNNEFGFDYLKDNMVTKKFDKVQRELNFAIVDEVDSILIDEAR
                     TPLIIAGEGDEDSKLYELANSFIKTIKEEDYELDRKDKTVALKESGIDKAESFFGIKN
                     LTDIENIELYHHINQALRGHKLMEKDVDYVVVNQEVMIVDEFTGRVMDGRRYTDGLHQ
                     AIEAKEGVEIKDESKTMATITFQNFFRLYNKLSGMTGTAKTEEGEFESIYKLNVVQIP
                     TNKPIIRADLHDKVFRTEKEKYQAVVEEVIRIHKTKQPILIGTVSVEKSEMLSGMLKK
                     EGIKHQVLNAKENDKEALIVSKAGKLGSVTIATNMAGRGTDIALGAGDKEEESRVKEL
                     GGLYVIGTERHESRRIDNQLRGRSGRQGDPGTSRFFVSLEDDVIKLYGGKSIEKLMKR
                     PNSNKNNSIESKSLTKAIERAQKGVEGKNFELRKNVLKYDDTINEQRKVIYNERNKVL
                     NDEDIQVDIQNMVKDIIKEAAAEYLTKRKRDYYGYFKHLYNTFMPADTLLIPDLDNKS
                     IEDIIDSTYEISKRVYDLKKMVVGLDKITEMEKIVLLKVVDQYWVEHIDAMEQLKQYV
                     GFKSYAQKDPFKEYAMEGYEMFESLNKNIRETTIQYLYKFN"
     gene            complement(12907..15087)
                     /gene="slpA"
                     /locus_tag="ACGI59_000266"
     CDS             complement(12907..15087)
                     /gene="slpA"
                     /locus_tag="ACGI59_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer protein SlpA"
                     /protein_id="HFG9738080.1"
                     /translation="MNKKNLAVVMSAVTVIGSAAPVFAAAEDSFPNGNTTVSSSKYSD
                     IERILKKYVADGVTGITVNFFDKKGNPKGVSISSLEASQISYVKDEIKDLKAGEYAKI
                     SVKITDGTEKKYDDTLLKELDSNAVVTKVGTATTVKIVDQDGNSIENDAKLKLQKYDT
                     TTSAYVDGATKDEVLAATKAAVKTSKFTDKTGNNATGTVAITATYADAVAGAIKLKYD
                     TQAAVAPTLDTAVASLDGTKEIYDFSKPVITVNTTDGTSKLSFEKTGEKVGAVESNVT
                     VIAASDETVTISGDAKEKAEALAKKYVFKDTELEDAYKTVTASDFEKTDNDYYEVVLY
                     PTGKRLNTASTYASSNYKPELPTSDRVDTPAIITLRSTNKNNLKSALDELRTYNNSYS
                     NNSVLAGDDRIETAIEISKDSYNADGGIKGDYVEANEVVLVGSQSIVDGLVASPLAAV
                     KDAPLLLTSKDKLDSSVKSEIKRVMGLDDKTGITSKKTVYIAGGENSVSKEVANELKD
                     MGLKVERLSGDDRYATSLEIADEIGLNHNKVFVVGGTGLADAMSIASVASNKEMPIVV
                     VDGKGKDLSTDAKDFIGSAYVDIIGGKSSVSEDMEDAIDDATGKSPERVSGDDRQDTN
                     AEVIKTYFEKDNSDSVISTGVKNFYVAKDGSTKEDQLVDALAIAAVAGHNEAPIVLAT
                     DSLSSDQSVAISKVTNSDDSKKLTQVGKGIADSVIKRIKDLLEL"
     gene            complement(15380..16978)
                     /locus_tag="ACGI59_000267"
     CDS             complement(15380..16978)
                     /locus_tag="ACGI59_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HFG9738081.1"
                     /translation="MKVNKRVLSIGLTISLIMAGAPNINALSSIEKIQGKDRYETSAL
                     IADKQIYDTVILVNTDNSIVDGLSASGLSGVVKAPIMLVQRDKIPTDVEKRLKDVKNA
                     YVIGTEDTIGKSVENQLKNKGIEVKRIGGEDRIKTSYLIAKEISAIKPVNDGDKVFLV
                     NGYTGEADAMSVSSVAARDGVPVILTDGKSIPFKVDGVQCYSLGSEEIMSNELVSKTN
                     SVRIAGKDRFETNKKVIQRFYKGTKKFYVSQGYKLVDAVAGSPLAKDKPIVLVNDGSD
                     KSVLRGADEVTSLGGMDKKVADQCISSASDKNTMPTITANDVEISVGDKFDNSMLNIV
                     ATDYYGNNLKPNIEGTVDTSKAGTYVLNVSAFDNFGQKCEINVNVKVIINTSTKDPNS
                     YEFKAMVSNEMYNLVNSYRKEKGKKSLRELDSLAGMANAWSKYMDEKKIFAHEIDGKN
                     AAEVFSGFGMRTDENIAYLPMNVKSVYTSKDAKEIAKSVFDLWKKSSKYNDNMLKEEF
                     YSFGFGMYVSNQREVNATMEFLNS"
     gene            complement(17197..18798)
                     /locus_tag="ACGI59_000268"
     CDS             complement(17197..18798)
                     /locus_tag="ACGI59_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp11"
                     /protein_id="HFG9738082.1"
                     /translation="MKMNKKILSLGLAVSLILVNFKSVNASSVVEKIYGKDRYETAAK
                     IADKQTYETVILVNTEKSLADGLSASGLSGATKAPILFTQQNKIPADTNRCLKNIKKA
                     YIIGTEDTISKSVEKELDSKNIEVKRIGGEDRLKTSYLIAKEIATIKKVDKVLLTNAY
                     SGEADAMSVSSVATRDGAPIILTDGKSVPFDVKNIQSYCIGSEEIMSNPLVKNTNSVR
                     IEGTDRFETNKNVIDYFFNSADGFYVSDGYQLVDAIAAAPLTKNSPMVLVNDGSDKTV
                     LEGAKNITSVGEIDEKVIQQCISASKSNGQPPAITVGSTEVYKGEKFDTGKLNIVAKD
                     NTGKVLPIEVDGFIDTNRVGTYILTLKATDEWGKSTGKRVEIKVLDDKSHDYNSPEFK
                     KMVSTEMYNLINSYRKEKGKEPLVVSSKLEGMANAWSKYMMDNKVFEHYIDGKNAPQV
                     FSEFGMRSEENIAYIYIDSKSVQTTQDAKDLAKAIFEVWKKSPEYNANMLSDEFYSTG
                     FGLYILSDGQVHATQEFLNGNEGSL"
     gene            complement(19165..21045)
                     /locus_tag="ACGI59_000269"
     CDS             complement(19165..21045)
                     /locus_tag="ACGI59_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp10"
                     /protein_id="HFG9738083.1"
                     /translation="MSKRKSFIRTIAVSTMAVAVTGSATCAYAAPVLQGTKTYEKVNT
                     IDISVDSVENIVYSFQASIKVQGEVEVVDNEQKEKITWSDNIKSQIKSGNANATCRAE
                     YNKSSNTTTLDIYVTSNEDLLDGNRLNIGRISVKKSGSNSNADYKVLGKGTNDKPALK
                     IVTYNNKTVDYENISSDEGLIFTLINESEVKPIGGTGSSKNDPEKYKVEKSEALEYLL
                     NNIRINYSIVSKETQESGSNVILKLGLAQKTTKGRKVTINKYVEVTLPKSLEYIVENE
                     LSKPDELPPDNGSGGNNGGGSNSGGSSSGGSSGGGNSSDSTSNVTVKKLKGDDRFETA
                     IKISQSGWTKSDTVVIVNGEDKSMVDGLTATPLASVKNSPILLSSNDKLPQKTVEELK
                     RLNPSKVVVIGGNNSMPNSVVEAIKAVNSKISVQRIGGDTRYQTSINIAKEIDKTNNV
                     SKLYIGAGNGEADSLSIASLAGKEKTPIVLTQKDGVDNEAEQFIKSNKVSNIYFIGGV
                     EKISNKAIEQVGKIANKDISNNRVAGQTRQETNAKVIDKFYSQSKLDGVVVANQDKLI
                     DALAVGPLAAKNNSPVILATNTLDKSQESSLKGKNSSKLFEVGGGIASSVIDKIKSLI
                     EK"
     gene            complement(21255..27218)
                     /locus_tag="ACGI59_000270"
     CDS             complement(21255..27218)
                     /locus_tag="ACGI59_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M60 family metallopeptidase"
                     /protein_id="HFG9738084.1"
                     /translation="MKKAISCVLAVSMCSAPLNVFAEPILEGKLRAVEKSVTEKLRGS
                     LEVDLNFSLPIKNSDSESMTNMQLRLKDDSNNSGIIKLGEKDSGTVNVGTDNIEYTIK
                     KLSASRSEIKNKDENISYYNIVFNNLPVGKYNVEVSGDGFKNKEIKDIDISSYSQRVL
                     LSNRASVDNKNKPLNYELFLMGDVNGDGKVDKSDYNKVLENIDSNKREFDLNRDGKVD
                     IVDLDYVQKNLGQSEDAKSLESIVSTNPIVDTSKVELKGNSDVDINGNVENLFSGENN
                     GVTISSKNDEISEEKPAVMDIALSEALNMEVINIKAPEGTAPAKGIVEVTDKDGNVQQ
                     IPYENKEYMKNLKDEEQQEEVNNEQEEVNQEEVNNKDENINQTDNNSNNKDENNDEQQ
                     ENSDKQEVNSNTNQDKNNDKENSSMDKPNSDEDTPKEEGNISKDDNSTTSTYSKSTLE
                     TYADVQNSDIVINLGKQVAVKKITIKITATTANDRNLAEISKVEFLNNVYKEIPKPEM
                     NIPKINKISTSTAVGNEKVSFYWNNEVNVSAYELILDKIDSKGKVLSTKKLQTSKNTI
                     EVRDIEAYGLYRVSIQSLNGDWSSGYKDATPENLDGVPENVDSNYKPIQFSPDSILEF
                     QVVPDTKPEPPEGINVKGKFKSLDVSWKNHKQAKDFDLYYKEKGSNGSWIKYNDKLIR
                     GTSSKIEGLKDNTTYEVRMTATNHLGTSGMSKTYIGTTESMDAPTTPNYRLINTPKEN
                     GGFGIPTNHIVDVTYPAGYDKSEYSDKNPFDKFNVVDGDFTTHWTFPTYNAAEGTNRG
                     PIITFDKSYTMDTFMATPRFDVSDKTINDFEVRYWDENGQLHNLGDVPFETKTDPVNN
                     NKYVMVRLKEPITATKIQVSVCSSYSYKSNISELKFYEYDSIEDDVRNLFMDDLQVEL
                     KEDVTQEKITELKDRLNTPDKASGEYHPYKTVIERELKLAQDLYNDRATLTDVTTVNQ
                     EINKLGKYKDKNGQYTVNSNNLGMQNDWQALGVAARAGDEITVYVGSKSGKTPKLIYT
                     QYYGESGAYKSGEINLNTGKNVIQLSKLHSLDIECGGSLYIRYAEDTPSGGDIKVRVA
                     GATKIPHLNLNGTINDKSPAGVSESKKKIKAYIEELARYKDAVKGEPYYPGTNVGNSY
                     GYSEKTGVLNTTDIESDKVTLNVPATAVYQGISKGNADLNTQVDRLYNSMLAWEQIMD
                     LVYSERGVFKTQDLDGNGTVDDNEFNLTKNDMAPKSRMNIKYQRMFIGAFMYASGLHV
                     GVEYGSVPGLMNGVPFQIDDSGKATGGNLFGWGIGHEIGHVTDIGKMTYSETSNNVLA
                     LLAQTFDDKTHSRIEGSTMDKIYEHVTSNSLGIPSNVFERLGMLWQLHLAYDDDFTGS
                     MLKNNSDADLSNDTFYAKISRKYRALSSSDPINSLPKDQMLVAMASSVVEKDLRDYFK
                     AWGVEITPELNSIMDSKNYEKESRKIQYVNDEARRKILNENIASMAKDTKVSASFSDG
                     IQSGSLVKSNKISIDLDVDKDKDKILGYEIIRSDGNYLDGENGTQVKYRTVGFVDAKG
                     NSKTKFTDNISPLNNRAFTYKVVAYDYHLNPTEEFEVGTVKLSDEGKINKSAWSFITN
                     TVSDGDVRTENDSHGPLQNPEIDNIKDNDANTTYKGKIISKAEWNKDPQKDPDINVEE
                     NPYIIVDMKETLPIVGLKYTKPETEARKFSLKGLFNFRKNTSTTYNPLTNYKIQVSND
                     KKNWEDVSQGTFEYGQDLVGGGKDQDNEARVTFNKDGKLWTYQARYVKLISNNKSNIE
                     VAELDIIGPPGDNIEIGTMNNADETRTNGIGKLAEDYVYQSDDSSTSEDETGKIPAGS
                     IVITGEYSGNPAFNLPLLIDKNNKTISGEALLFAEVPEKGELGEISKGTWVYYMPKEN
                     FDSLSDKVKAELYRYNDLQGDTPVGQRFVSDTMYVPVGAKNYDELKTISLTDSNSKAR
                     KATRNSIDISNKKVITVSNKVKGNIVRDK"
     regulatory      complement(27342..27427)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(27599..29002)
                     /locus_tag="ACGI59_000271"
     CDS             complement(27599..29002)
                     /locus_tag="ACGI59_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp9"
                     /protein_id="HFG9738085.1"
                     /translation="MKNSKKILAIGLTLFLVMVNTPMVNALTSVEQIKGNDRYETAAK
                     IADKQNYNTAILINSDNSLADGLSASGLAGALNAPILMTKQNQIPNTTMERLNKAKTV
                     YIIGSESTISKNVENQLLSKKKVVQRIFGENRFDTSIKIAEKIKEIKPIDKVIIANGF
                     TGEADAISASPVAARDGVPIILTDGNSVGFDTTGLKSYALGSSEIISDELVKSTNSIR
                     LGGTDRFETNKIVIQEFYKNSKEFYLSKGLQLTDALAASTIAKNAPVVLVENGSNKSI
                     LSGADKLTVLGGINQNVIKQCINQASPNQQGLYYNPNDRAFKERIKGKVYALTKQYRK
                     ENGVRALSVASRLEGLANDWSNLMANKKTLSHTINGKNSYSTFLKYLDWSEIKPGYIA
                     VQGENIIKYKIPDKPVYTNRDADDIGNFIFNEWKTNPEEGTNMLHKGYEIMGFGIAIT
                     GDKNLYATHEFYGRYKE"
     gene            complement(29464..31359)
                     /locus_tag="ACGI59_000272"
     CDS             complement(29464..31359)
                     /locus_tag="ACGI59_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp8"
                     /protein_id="HFG9738086.1"
                     /translation="MLSNKKRSMAIVMAGATVMSAAAPIFADNTVTENVDKNYTVSAK
                     DSAKLIEEVRKALEVKFEDTKAGANVNDRVYDIKVDNVNLTNATQLQNKINSLTEGQS
                     LKVTIQDKGHQVLGGKVVDYKIENYKTAQEIVDAVNAYNATLAEDSDNKLTATIKSTN
                     TVEVKRAKDSANVITLNVGDQHLDFSKVITSEEGTFEGYEKSYSDIDSKELHTITVKN
                     ADLQDISAEELFDGIRLTTLGREIVNKVKNGYALTFENEAILTQEQEDSDDKDKPEKS
                     SFDIVLSKANEKPETISVSSKNHKLVRDLHKVLTDVKGGKELKVEVLSGDSRFTTAVE
                     VSKERFKDGEAEAIILVGEDAIVDGLASAPLASQKNAPILLSKKDSLPSEIEAEILRV
                     LGSNLSSKKIYIVGGESKVSKETEEKLSKLGVSKVERVSGEDRFETSLEIAKQLKDTF
                     KTAFVVGGNGEADAMSISARAAQFGAPIIVTGNELDANAEKLLKGKELEIVGGENSVS
                     KEVEDKLVDIDLNNKVERLAGENRKDTNAKVINKYYAGATKAYVAKDGYVGGNGQLVD
                     ALTAAPLAASSKAPIVLTTEELSKSQEEVVELRLKNATKLVQIGEGIAKNAIEKIAEK
                     INLFTKN"
     gene            complement(31985..32128)
                     /gene="scfA"
                     /locus_tag="ACGI59_000273"
     CDS             complement(31985..32128)
                     /gene="scfA"
                     /locus_tag="ACGI59_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="six-cysteine ranthipeptide SCIFF"
                     /protein_id="HFG9738087.1"
                     /translation="MENKHVKTLSQATLKQSAAKGGCGECQTSCQSACKTSCTVANQE
                     CER"
     gene            complement(32194..32556)
                     /locus_tag="ACGI59_000274"
     CDS             complement(32194..32556)
                     /locus_tag="ACGI59_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04086 family membrane protein"
                     /protein_id="HFG9738088.1"
                     /translation="MGKSNYIFKGLGYAYIITLAVLLVYNLFLTFTDIGGDNITMVSS
                     FITTISAAIGGFYTSKHMKEKGLMYGLLVGLLYIVCIFLTVFLAQEKFVFEVGMIYKL
                     LLISAAGGIGGVLGVNFK"
     gene            complement(32614..32907)
                     /gene="yajC"
                     /locus_tag="ACGI59_000275"
     CDS             complement(32614..32907)
                     /gene="yajC"
                     /locus_tag="ACGI59_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFG9738089.1"
                     /translation="MPQQQIIMSIGLWVVVIAIFYFLMIRPQKKKDKQLKEMRSSLSV
                     GDKVITIGGIVANVAKVEDDVVILELGPNRTKVPFEKWAIGTVKSKKEEIEED"
     gene            complement(33041..34162)
                     /gene="tgt"
                     /locus_tag="ACGI59_000276"
     CDS             complement(33041..34162)
                     /gene="tgt"
                     /locus_tag="ACGI59_000276"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFG9738090.1"
                     /translation="MYAVRYELIKTCKQSGARLGRLHTPHGIIETPIFMPVGTQATVK
                     SMTPEELKEIGSQIILSNTYHLYMRPGHELIKRAGGLHKFMNWDKPILTDSGGFQVFS
                     LGPLRKIKEEGVEFRSHLDGSKHFLTPEKAMEIQNALGSDIMMAFDECAPYPSDREYV
                     KNSLERTTRWLKRCKDAHNNTDKQALFGIIQGGMYKDLREQSAKEITNIDLPGYAIGG
                     LSVGEPKPLMYDVLEHTTPLMPKDKPRYLMGVGSPDDLVEGVIRGVDMFDCVLPTRIA
                     RNGTAMTSQGKVVVRNATYAEDFTPLDPECDCYACKNYSRAYIRHLIKANEILGARLI
                     TTHNLHFLLNLMKQIRQAIMEDRLLDFRNEFFAKYGYEI"
     gene            complement(34241..34726)
                     /locus_tag="ACGI59_000277"
     CDS             complement(34241..34726)
                     /locus_tag="ACGI59_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible PsiE family
                     protein"
                     /protein_id="HFG9738091.1"
                     /translation="MHSLQKQKKNNPILKIAFIFERVLAVVVLIAVFLGTIDVLRLMW
                     GAYIVDFQNPVQYSQLNDFLAQILLLVIGVELVVMLSLHIPGAFIEALLYAIARKMLL
                     LPKNEGMIDVLIGVIAIAGLFAIKKFLVTKDKSALNIMSIHDEEAIKEQQNKEDEDKS
                     I"
     gene            complement(34738..35763)
                     /gene="queA"
                     /locus_tag="ACGI59_000278"
     CDS             complement(34738..35763)
                     /gene="queA"
                     /locus_tag="ACGI59_000278"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFG9738092.1"
                     /translation="MKTSDFKFDLPQELIAQVPIEDRASSRLMVLDKETGNIEHKVFR
                     DIIEYLNPGDCLVLNNTRVIPARLIGEKLETGGKIEFLLLKRTEEDTWQALVKPGKRA
                     KVGTKFSFGNGKLIGEVVDLSDEGSRIIKFHYDGIFEEILDELGNMPLPPYITARLDE
                     KERYQTVYSKHNGSAAAPTAGLHFTEELLNKIKEKGVDIAFVTLHVGLGTFRPVKVED
                     VLNHKMHSEYYMVSQEAADKINRAKENGKNVICVGTTSCRTIESACNEDGKMKETSGW
                     TEIFIYPGYKFKVLDKLITNFHLPESTLIMLVSAICGKDNVLNAYNEAVKERYRFFSF
                     GDAMIIK"
     gene            complement(36081..37100)
                     /gene="ruvB"
                     /locus_tag="ACGI59_000279"
     CDS             complement(36081..37100)
                     /gene="ruvB"
                     /locus_tag="ACGI59_000279"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFG9738093.1"
                     /translation="MQGFEDENRIITSTMKMEDIDIENSLRPKTLEDYLGQEKSKEQL
                     SIFIEAAKSRNEQLDHVLLYGPPGLGKTTLASIIANEMGVNLRITSGPAIERAGDLAA
                     ILTNLNENDVLFIDEIHRINRSVEEVLYPAMEDFCLDIIIGKGPSARSIRLDLPKFTL
                     IGATTRAGMLTNPLRDRFGVICKLDYYTVDELSKIVLRSSSILDAEIQSNGALELAKR
                     SRGTPRIANRLLKRVRDFAQVRADGKITDKVAKDALELLGVDSLGLDFVDEKLLMTII
                     EKFRGGPVGLDTLAASIGEDRNTIEDVYEPYLLQLGFINRGPRGRVAMPLAYEHLKIP
                     YPNEK"
     gene            complement(37207..37809)
                     /gene="ruvA"
                     /locus_tag="ACGI59_000280"
     CDS             complement(37207..37809)
                     /gene="ruvA"
                     /locus_tag="ACGI59_000280"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFG9738094.1"
                     /translation="MYSYIKGTVEEIYIDSIVVENNGIGYKINVSSNTIMNLQVGEAT
                     KIYTKLIVREDDMSLCGFVSREELKMFELLTSVSKIGPKVALSILSFASPAQLGAYIL
                     SEDIGKLSKAPGVGKKTAERIVLELKDKVDKNNIEFEPTLLSQKPTLISQDESVDALV
                     ALGYTLSESKEAVQKCKKDGMNTEAIIKKALTYIMSKSLK"
     gene            complement(37802..38311)
                     /gene="ruvC"
                     /locus_tag="ACGI59_000281"
     CDS             complement(37802..38311)
                     /gene="ruvC"
                     /locus_tag="ACGI59_000281"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HFG9738095.1"
                     /translation="MIILGIDPGIAIVGYGIIEYKNSKFKAIDYGAVTTPAHMNISRR
                     LELVYKGIDTIVKNYNIDEVGMEELFFNKNVKTAITVAQARGVTMLACAHNGKPVYEY
                     TPLQVKQGVVGYGRADKAQVQQMVTSFLSLKKVPKPDDVADALAVAICHAHSNKLEKT
                     LKNIGGKYV"
     gene            complement(38623..39252)
                     /locus_tag="ACGI59_000282"
     CDS             complement(38623..39252)
                     /locus_tag="ACGI59_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9738096.1"
                     /translation="MFEKKKKELFPWKISALILVGVFVLSSGVYIGTRLKHNGVQTGE
                     VVSTSANMNEKSKEAFGLNKNCEVWLQSEGENETNYKRTLTMAGIVPESLLDKTKSEI
                     VSYFKKEYPTKKIKSMDKNEIILVESKYSDNKDVQAMDVTIKGKYTIENDNGNIALYK
                     YDDSGSKTLVEKTKIRVDSLPKTVQDEIKKGVVMDTEEEAYSRLEDFAS"
     gene            39455..39778
                     /locus_tag="ACGI59_000283"
     CDS             39455..39778
                     /locus_tag="ACGI59_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HFG9738097.1"
                     /translation="MERINCNVINCSHNNSHICYADKIFINGEKAHNDRHTCCSSFLD
                     ESIYSNLSSNVNNKNCEELVCTVKNCKYNKDGSLCTLDNILVAPETDRVNLYIETCCS
                     SFECK"
     gene            40046..40900
                     /locus_tag="ACGI59_000284"
     CDS             40046..40900
                     /locus_tag="ACGI59_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease"
                     /protein_id="HFG9738098.1"
                     /translation="MSTFYIQFLYFVIYSFIGWCCETTYCSVLQKQFVNRGFLNGPFC
                     PIYGFGALAIVATLTPFVHNIPLLFLFSIIITSIMEYCTSFILEKIFNMTWWDYSKHK
                     FNIHGRVCLENSLMFGVLSLIVMLIVHPIVVDFINSISKNVLFIFAISIEIYFVLDLV
                     ITVHTILQLNGKLKQINLIIKELKDKKEYYKLITQETIENKLESLVENRLDTLEDRFE
                     ILKEKENYRYAKNRIDELKNKLNTLLSNHKLLHRRIIKAFPNISSNKHTDILNKIKEN
                     IKSIKKDK"
     gene            complement(41086..42522)
                     /locus_tag="ACGI59_000285"
     CDS             complement(41086..42522)
                     /locus_tag="ACGI59_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HFG9738099.1"
                     /translation="MREVKPISIDILNTFKQVDEDRLNKLLADELKHLDRKIVVLDDD
                     PTGVQTVHDISVYTDWDKDSMEQGFNEKNSMFFILTNSRGFTVAQTTKAHKEISKNIV
                     DVSKKVNKDFIIISRSDSTMRGHYPLETNLLKSEVERLSGKLFQGEIIMPFFKEGGRF
                     TIDNVHYVKEGEILVPAGMTEFAKDKSFGYKSSDIGEWCEEKTNGKYKSSDMIYISSK
                     ELRELNIEAITEKLKKAEGFNKIIVNATDYVDVKVFTIAFIRAVNGGKEFMFRSAAAI
                     TKVLGGVSDKELLTKDELVSKGNTNGGIILVGSHVNKTTQQLEELKNCKYPIEFIEFN
                     QHLVLQENGLKNEVKRVIKVVEEKISSGKTVAVYTRRERFDLDTNDKDKQLMISVEIS
                     DAVTSIIGMLNVRPNFIIAKGGITSSDVGTKALRVKKATVMGQIKPGIPVWMTGKESK
                     FPNMPYIIFPGNVGEISTLRESVEILMG"
     gene            complement(42593..43975)
                     /locus_tag="ACGI59_000286"
     CDS             complement(42593..43975)
                     /locus_tag="ACGI59_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate:H+ symporter"
                     /protein_id="HFG9738100.1"
                     /translation="MITGYGLLIAFVISIGILLVSIIKFKVNPFLALLITSIITGFMV
                     RMPINEISTTISTGFGNTLGSIGIVIGLGIIFGNILSESRATESIAKGLLAKTGEKNS
                     ALAVTTAGFLISIPVFMDAAFVIMMPIIKYVSRVTKKSLMVFVCALGVGTIVGHALVI
                     PTPGPLAVAANVNANVGSFILYSIIVAFPAALIGGWIYGKRFEKYPAYAIDENDREKN
                     LEQGKDSIKIEDDDSSKVPGFGISMFSLLFPILLILISNVFSMFLEKGSTMSGVLAFI
                     GDKNIAILLGILVAIGFLKKYINKPMGDVVIEAADSAGLILLITGSGGAFGSVINASG
                     IGNFLVDTMSGLSISVVVLGFLLSALLRISQGSATVALVTTSSILGPTILATGMSPVL
                     VGLAICAGGVGFSLPNDSGFWVLSRFSGLSVKDTLNSWTIGGTIAGVVAFIMVLLLSV
                     INGIIPLPGL"
     gene            complement(44111..45019)
                     /gene="garR"
                     /locus_tag="ACGI59_000287"
                     /pseudo
     CDS             complement(44111..45019)
                     /gene="garR"
                     /locus_tag="ACGI59_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426591.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
     gene            45412..46083
                     /locus_tag="ACGI59_000288"
     CDS             45412..46083
                     /locus_tag="ACGI59_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFG9738101.1"
                     /translation="MATFKRLQEQAYDYLKELILSGEMIENEIYSETKLASEIGISRT
                     PIRDALQRLSQDGFIDIIPSKGFRIHQITANEIVEIFQIRSAIEGFCTFLITSQYKEA
                     RAVETISKLKHLLDKQKDILLGDKNLNSFAEYDTLFHTTIVSYAQNTEFDKMFNNYMY
                     RIKKLALDSLSHEGRLETTLKEHTDIFNNIANGCIEDIYRTTLIHMETPKYINLEDFC
                     NNKLF"
     gene            46186..46734
                     /locus_tag="ACGI59_000289"
     CDS             46186..46734
                     /locus_tag="ACGI59_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9738102.1"
                     /translation="MIFLNEDVVTNETIDKDYAIEEVRMACKHFGDLYFYFSKVLFEE
                     FGEDKTSEILRKVLFERSEERAIAMRERAHENGDELIADNIISTTDVPFLGWVPEFEE
                     LHCPYGASWLLRFEENPWFKKFASLYCDVTDTTVAEVFTGDTSHKITKNILWGDKSCE
                     RIYFHDDKVSEGKYTYGNKNCK"
     gene            46996..47304
                     /locus_tag="ACGI59_000290"
     CDS             46996..47304
                     /locus_tag="ACGI59_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9738103.1"
                     /translation="MFNKIYEKKEFIVFQVKKGYVVYNTRKSFEEGHTHLKHFEAAKT
                     AIDLAINKKIPRSKDGYYLTSLIRISDDGYYIDKLSELLYVREQKGKKEKYCNSGYQM
                     "
     gene            complement(47466..49256)
                     /locus_tag="ACGI59_000291"
     CDS             complement(47466..49256)
                     /locus_tag="ACGI59_000291"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9738104.1"
                     /translation="MKKSKGFKKTIGRLLPFVKKYKFSFIIAIICIISAATMNALAPK
                     TEGLIITQLTKDVISIAKGVPGASVNFDYVTKILVILACIYFANAIFTYTSSFLLTNA
                     IQNTMRDLRNEVENKIRRLPISYFDSNSFGDVLSRISNDVDTISNALQQSFMQIVNSI
                     LVIILALSMMFTINIYMALIALFIIPISYFVSKFVVKKSQSRFSLQQNALGKLNGKVQ
                     EMYTGFNEIKLYGKEEDSIKEFKKVNQELCENGFKAQFISSMMNPMVSLVTYFGIAAV
                     AVVGSIYAVSGGITVGNLQAFVRYIWQINQPLSQMTQLSTVIQSSFAAIERVFEILDE
                     EEEIPDVENPVKIENVKGNVTFEHVNFGYGENSTLIEDLNAEVKSGQMVAIVGPTGAG
                     KTTLINLLMRFYDVKKGAIKIDGVDIRDMKRKDLRSMFGMVLQDTWLFNGTIFENIEY
                     GRFGATKEEIIQAAKVANVHHFITTLPDGYNMFLNEEASNISLGEKQLLTIARAFISD
                     PSILILDEATSSVDTRLELMLQKAMRNLMNGRTSFVIAHRLSTIRNADLILVMNNGSI
                     IEQGNHDELMEKGGFYEKLYNSQFADKESE"
     gene            complement(49256..50983)
                     /locus_tag="ACGI59_000292"
     CDS             complement(49256..50983)
                     /locus_tag="ACGI59_000292"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9738105.1"
                     /translation="MKLILSYLKNYKLLIVLNILAIFSFALVELGIPTIIAKIIDNGI
                     ANQNIAYIKQMGIVIVIISIIGVVGSILLGYCSAKVSTSVTRDIRNDIFEKSQEFSHT
                     EYNKFGISSMITRTTNDAFQIQQFVNILLRTALLTPVMFIISIIMTIRTSVELSIVLA
                     ISVPFIIIGVAIIAKVSQAISSKQQKGLDKLNLISRENLTGIRVIRAFGNDDYETERF
                     EKTNTYYANVSKKLFKLMSITQPAFFLLLNIAVLAVFWISSEKINIGELQVGQLVAFL
                     EYLFHAMFSIMLFSMVFIMYPRAEVSANRIKELLNEEPLIKNPENGIKDTENKGIIEF
                     DNVTFTYPDGEASVLKDISFTAKTGETVAFIGSTGSGKSTLINLIPRFYDVTEGSIKI
                     NGVDIREYDLKALRKKIGFIPQKSLLFTGSIANNIRFGKHKAGESELEYSAKVAQAYE
                     FISKKPRKFDELISEGGANVSGGQKQRLSIARAIIRRPEIYIFDDSFSALDFKTDAIL
                     RAKLKKETKDAIVLIVAQRISSIIDADKIIVLNEGQVVGMGTHKELLKNCEIYYEIAT
                     SQLKKEELE"
     gene            complement(51228..51494)
                     /locus_tag="ACGI59_000293"
     CDS             complement(51228..51494)
                     /locus_tag="ACGI59_000293"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HFG9738106.1"
                     /translation="MNLEPKKLIEQIGKEKDKKDIWIVGGGDIVKLFMKDNLIDDYYI
                     YILTIILGRGISLFKSGIDKTNLNFKKASNIGELVKLEYSKKQK"
     gene            51792..52904
                     /locus_tag="ACGI59_000294"
     CDS             51792..52904
                     /locus_tag="ACGI59_000294"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFG9738107.1"
                     /translation="MKISEVATPNFLLDLDQLEKNIDKIQQICTNNNKQLWPMLKTHK
                     STYIARLQKNAGASGFLVGTLDEGEALIKAGFEDIMLAYPYMGEVNINRIVTMASKIN
                     LICATDNIECAKAYSEAFSKSKIECKLLIIVDCGLHRFGVEPEKVVELATEISKLENI
                     KIVGISSHPGQVYGCSSPEGVPDVCVQEDSSMEKAYKALTDNGFNIEIVASGSTPTVE
                     SEAASKTITAVRPGNYVYYDAIQIGLGCATEDMCALTVVCTVISKNSSGYYLIDCGSK
                     CLGLDKGAHGNSSIVGYGRVCGHPELTIDSLSEEVGKIKVNGETDIKIGDRIRIIPNH
                     SCSTANLTSNLLGFRGDTIEKVIEVDIRENSKQIIL"
     gene            complement(53268..53777)
                     /locus_tag="ACGI59_000295"
     CDS             complement(53268..53777)
                     /locus_tag="ACGI59_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HFG9738108.1"
                     /translation="MSQQNTGNVIVNSKPKKTAVEIFMGGAKKGFYIGVEQILPAMIM
                     GYVIVQFLQLTGLVDILGNIFGPVMGIFGLPGESVVVLIAAFFAKAAGAATAANLFAQ
                     GLITAPQATILIMPCMLMGTLVGHYARIVLVANASTKYRMLLLAVPIFDAIVGMLIVR
                     VLLTVMGLM"
     gene            complement(53779..54426)
                     /locus_tag="ACGI59_000296"
     CDS             complement(53779..54426)
                     /locus_tag="ACGI59_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HFG9738109.1"
                     /translation="MDVTAGDNKVTWKGYLSLFVLIVLFSGVFKDSQGFLKALDFANL
                     TGSFGTIADGVDFMGKGGTGAKDGFLFALTLIPTVCFAVGLIDVVESMGGMKAASKIF
                     NPLLRPLLGIPGIAGIAFVSSFTSSDVASIMTKELFESGEITDDERTIFVAYQYAGSA
                     VILNTINTQAPLLPIALLALGPIILIEIFCKILGANLVRLIIGVRNKKNKAKEAV"
     gene            complement(54440..55612)
                     /locus_tag="ACGI59_000297"
     CDS             complement(54440..55612)
                     /locus_tag="ACGI59_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFG9738110.1"
                     /translation="MNTYLNEISKFVDENREEIVSLWKEIVNMESYTHCKESVNKLAK
                     RLKFEFEKEGLDCELVDVGDNGSTLIGTLGSNIDKKPIIFSGHMDTVFETGTFGENPF
                     KIIEGKAYGPGVLDMKGGIIISLYAIKALNKIGYKERPIKIVFSGDEEIGHKDSTGAD
                     VILREAKGGLCAFNMETGLVDNSLCVGRKGRIGCNIHVKGVETHAGNDFEGGRNAIEE
                     MANKILRIQKLTNLDVGTTVSVSVIKGGRVENSIPEDCSIKVDLRFEKVEEMENVKEQ
                     VREICKETYIEGTSTEVNFISEMMPFETTEDVMRFHTFVNEVSKENGFGELNAKRLGG
                     SSDASYLTIANVPTICSFGVRGEWNHTSREYAVVDSMFERVKLISTVILNLDKFEV"
     gene            55880..57256
                     /locus_tag="ACGI59_000298"
     CDS             55880..57256
                     /locus_tag="ACGI59_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9738111.1"
                     /translation="MKYKIGFLTTSYFMSDKFKQALKELNDTCEITFIELKGENPFNN
                     LPNIYYEQVDNFDGFVCSGIVPYSELIINIKDIKVPLNFLKLDEKDFYKYLFKLLNSQ
                     KDIDFTKSFMDFLREDNNYYDIYSLIDSNKCPYTIKDFNIPQPVYDFKKLNDKLLEIH
                     LDLLRDNKINLSFTRNYIISCELNKLGYDSIYMVPSTESILNTFKSLVNEITLQNLDK
                     NKSATCIVTVNSSEYINEDLIFKNDEIQNQIYNTILNSLSRHGFYGVQVKKNDMKIEI
                     HTTKEMLNNMTNNYTDFFISEDLKEIKYSLNIGWGIGNTNIHAEQNARQANGKSASLN
                     GNCTFIVNDTNDTIGPLYSNKNSNNIEDSSIANKVASFIPLSNTNVSKIMCMINDRNS
                     NNVSAEILADYMNITLRSANRILSILYKAGIATIVNTKLDNQRGRPKKIYKVDFLSFL
                     NKMQKLNS"
     gene            complement(58192..58722)
                     /gene="bilS"
                     /locus_tag="ACGI59_000299"
     CDS             complement(58192..58722)
                     /gene="bilS"
                     /locus_tag="ACGI59_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein BilS"
                     /protein_id="HFG9738112.1"
                     /translation="MKSLVLYSSLTGNTKKIAYTIYDEIQEEKDIKDVNELVDYGIDY
                     ENYDIVFLGYWVDKGICDKNSKQVLENIHNKKIALFGTMGASKKGSYGASIIEKIESI
                     IPKDNEILGSFICQGKIAEGLKAKYKEMLKLSPDNEHIRQQLNNHEESQSHPDEQEIY
                     EASMFAKNMMIKASIV"
     gene            complement(58810..59904)
                     /gene="bilR"
                     /locus_tag="ACGI59_000300"
     CDS             complement(58810..59904)
                     /gene="bilR"
                     /locus_tag="ACGI59_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bilirubin reductase"
                     /protein_id="HFG9738113.1"
                     /translation="MYCNLLKPIKIGNLELKNRVSFAPTSMGLKLEEKIKKFSDIAKS
                     GVALITLGDVSIRPSFHKVAISLSDEDGVMKYKKIVDEIHNSGAKVSAQLFCSDYDVN
                     LIKDTMNMGITSHDEIKKIMNDGVKDYITNMPKEEIKNIINLFKVTALNAKKAGFDMI
                     QIHGDRLVGSFSSSIFNNRNDEYGRTCDNRSRFASEIISSIRDEVKDIPIDYKFAIRQ
                     ENPHYGNAGVLLSEVEYFVKKFESLGVNSFHVTLANHSKLEDTIPTNNHPYFKEEGCF
                     LYLADEVKKHTNLPVCGVGKLSSPDFIESIISNNRVDMVSMSRQLLADSNWLQKVKDG
                     RVDEIKKCCYCNKKCADALQTRSQFGCILD"
     gene            complement(59906..>60367)
                     /gene="bilQ"
                     /locus_tag="ACGI59_000301"
     CDS             complement(59906..>60367)
                     /gene="bilQ"
                     /locus_tag="ACGI59_000301"
                     /inference="COORDINATES: protein motif:HMM:NF045593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bilirubin utilization transcriptional regulator
                     BilQ"
                     /protein_id="HFG9738114.1"
                     /translation="EGKFKMDFKNLQYESLSYIICNLQKNFKLYCEKCLKPYKLTNGL
                     YFYLIYINKNRNCSLNDVSTEFEVDKAHTTRTISRLEQDGYIEKIQNPNDSRAFQLRV
                     TDKGEEVLGDIKNIFSKWDNHIKKEFSDTEYKELVKNLHVVKDIKTAVEEE"
BASE COUNT        18758 a        10780 c         6730 g        24099 t
ORIGIN      
        1 agattatttt tctttattct agttttgtca atacttttat ttattttttt gttacattca
       61 attccaatcc ccatgttacc tccccaaagc ttattcaaat atatactaaa ctacatattt
      121 atctataaat ctatttgtat ataaacctgt ttaactacat aactttagaa caattacaac
      181 aaacacttca cttaaatata tatattctta gctaagaaat ttctttagaa atccaagctt
      241 atctagtata tcttgtattt tattgacttt ttgacttata gaatctatag ctttttcaat
      301 tgaatctact ttagcctcta aatcttcaac ctgattaact tttttatcta tagtaccatt
      361 attagttata tcactattag atgaattatc tataagtttt cctatatatc catcattaat
      421 caattttgta tcattagcag aagctaatat catcaacgta tctatatctg aatttctagt
      481 attttctata actctaccat ttttgtcacg aactgacaat atagtgattg tatcatggtt
      541 attaaatcca ccttctccaa tatttctaac tataacattt accaaagtag catagttatt
      601 cacttcacca ttaggcatat ctactgtaag agtctttgaa caatcaccct ttatattcac
      661 agttattggt ttatttgtta ccattctaaa agcttcattt acagtattct cttttggttt
      721 aaagtttata atatcattag gacttgctat atttatcata tcagttaatt cctcaacagt
      781 ttttgcttca aacacagttt tttcttgtag acttttaatt tcatcaaaag cttcttcatt
      841 tccatttcca cctacctgtg ttatagtatt taatttttta gtactaagta tgtccctttg
      901 ttttgtgttt aatttattac caaccaaaac aacaggagac tcattttttg cagccaatac
      961 tccaacagct aacgagtcta ttagttgatt ctcattttta ctaccatcct tagttacata
     1021 taaattactt aaatttgtat cagtgtaaaa cttttctatg accttagcat tagtctcatt
     1081 tctatctttc ccactcactc tttctgcatt aggaagactc ttttctacag ctcttgaaac
     1141 agaatttgta cctccaatta catatgcttt tataactttc tcatccctta taaatttatc
     1201 aaatgcttct actccgtcct taggactaga caaaattata ggcatcttat tttgagctgc
     1261 tggtgcccca acgctaacag catcactcaa tcctttttca ccatttacta cagcaacttc
     1321 cttaatatct tttatatcct taagcttatt agccaaaatc agtgaagttt catatctatc
     1381 cttaccatta attctttcta cattaagtcc attacttttt aacttgttct ctatatcttt
     1441 attaagtaca gcagtacctc ctatcaaata aatatcttta gctcctagac gttttatctc
     1501 tttttcagtc ctatcatcta gcttatcact ttctgtcaaa agtattggtg ctcctttagc
     1561 ctttgcaaat ggtgtagctg aaagtgcatc agctaatgaa ttgtcattaa ctagcactac
     1621 attaggacta gaactgtatg ccttttgact tatctttaca gcagtttcat atctaccatt
     1681 acctattaat ttttcctctg taagtgcata tgatggtatt atagaaatgc tacttaaagt
     1741 aagcgctatt gtcagtattg ttaaaatagt ttttggtgct ttcattatta ttccccctta
     1801 ttttgtttaa aatcttacta tatttatatt ataaaataac tttacactat tttacagttt
     1861 aaaattatta catttttcat caataataag aaaactatct aaaaatatag acaatcctat
     1921 ttttcattat ttaaatgata aaaatataaa acaacaaaaa agagtgcctc atgaactaaa
     1981 aagttcattt tgagacaccc cgttttgtta ttttatattt ataaacttct attgaatatt
     2041 ttttaataat aaaatgtata tccttactat ctttccattc actattttcc taaaagagta
     2101 tttagttcat taaaagcatt ttcatttcca tttccaccaa ctctaatagg agtttctatt
     2161 ttcttagact taagtacatt tttttgactt tcatctaact tatttccaac taaaactact
     2221 ggtgaatcag ttttagcacc taatgctcct actgctagtc catctatcaa gtctccttga
     2281 ctctttgaac catctttaac aacaaaagca tatttcaaat ctgagctagg atagaattta
     2341 tctattatct tagcattagt ttcatttcta ttacttcctg caagtcttgt tggatttgaa
     2401 agcttacttt ctaaatttga tgataaagta gctgttccac caattatata agacttagtt
     2461 attttagatg aatttaaaaa ctcatcagca ccttgcaact cactcttttc attagtaagt
     2521 attattggca tattgttatc agcagctgct gcaccaacac ttattgcatc tgcaagtcca
     2581 tttacaccat ttgccactgc aacttgtgaa acagatttta tactatttaa cttttgagct
     2641 agagatatac tggtcttgta tctatcacta cctgaaattc tttctactga tatacccata
     2701 ttactaagtt gagtttgtac attcttgctt aaagaatttt ctccacctat tagataaact
     2761 tttttagttc ctaatctttg taactctgcc ttagtttttg aatctaagtt atcttttcct
     2821 gtcaaaagaa ttggagagtc aattgctgct gtaaatggtg tagctgttaa agcatccact
     2881 attgcatctc catttactaa tactgcattt tgtgaagaag tccatccttt ttgactaact
     2941 ttaactgctg tttcatatct atctgctcca gttaaagatt ctcctgaagt agcttgtcca
     3001 gaagatgttt ttgttatagc ttttataaca aagttacaag gagaaaattc actatatgta
     3061 tttatatctt caaatttatt gttttgaagt atgtagcttt cacctaagtt tgctttagcc
     3121 tcatatagat agtatccatc tatatcagtt tcatatgcta aagttgattt ttctctatta
     3181 ggatttgctg tgttgtctat aactactact attgctcctt taccttttgg taagctgtaa
     3241 gaattagtag gtacatttat gtatccagaa tatgatactg ttccacttgc aagttttgtc
     3301 attgaattgt tttgaggaac tccatttact actggtgcat aatatacttc atattttgct
     3361 cctacagaat ctgtttcaaa cataacagag tcaagttttt cagaatctct gctaaaatca
     3421 aatacatttg ctgctgttac tttatttgac attatcttgc taagaccagc atattcaagt
     3481 tggtacattt ttttatcgct atctggtttt gatactgatt tcattgcgta gttatctgta
     3541 tcttttaaaa gagttttgtc ttcataagaa atccagaaaa agcctttttc tgagttaaat
     3601 tctccccaac tactttttac taaccatgct ccatttgatt ctggtctaac acttggtttg
     3661 aacttatctt ttgaataatt atcatcccat cctacaattg aaacagaatg atttaaaggt
     3721 tcacttttat cattacaatt atacgaattt tcatcatcac ttaagtaggc tgaatagtgt
     3781 gcatatcctg atgtcactgc accatattgc attattgcat ttttaacaga agttttatct
     3841 ttactaattc ttacaacatc tgttacatta aattgtgtag gtgcagtatc catattttga
     3901 ggcctagttg ctccttcatc ttctgattta aaattatatg gaatgtcttt ttccagctta
     3961 ggtcctgccc aggctgtaag atatccaatt gcagttacat tcgaactacc tgtcatatca
     4021 gttaagttcc atccatgtat tcctccagtt gaccaccatc taaaatgctc ttctgaaaga
     4081 tcataagttc cataaccttt taatttcaaa tatgcttcta gtgttgacat tccagaaaaa
     4141 gcccaacatg tattaagttt tccttggtct tttgctggtg tagttaaatc taattttctt
     4201 ggatcatatg atacacttga tttatatgat gctggaagac tacttcttac attagtttta
     4261 agtgttgttc cttccatttc ataaggagtt ggtattactc cattatattt tgctatatct
     4321 ccatttttta catcttccaa gtattgaaga tatgattcag aatactctgc agtttctact
     4381 ccagtcagaa ttttattttt ttctgctgag acaggtattg ttgacacaat taaaaaacaa
     4441 actgtcaaca acaccagtag ctttgacaat ttttttaatt tatactttct catatttacc
     4501 cccttgtatg tatttaattt ttattatatc agatttctaa atctaggtca tattttattt
     4561 ttataaaaat aggaattata attatacata tataaaatac tattttatac tgcatattta
     4621 ttgattaata tgtggtatac taattacata ataatatata ccaaataatg gaggaaaatt
     4681 ttttgatatt aaaaaaacat ctagaaacaa tattatatct tttttttggt gctttaacag
     4741 ttttaataaa cattattact tacctatttt ttacaagaat tattaatttt acttttttat
     4801 tatcaaactc tctatcttgg tctatagcag ttttattttc atatataaca aacaaatttt
     4861 ttgtattcaa aagtgaaaaa actgagtttt tatttctatt aaaagaattt gtatcttttt
     4921 tatcttgtcg tttattttct ggttttatag aaatattact tatgtattta atgattgatt
     4981 tattttcttt aaatgatatc ttagtaaaaa taggcactaa tataattgtg ataattttaa
     5041 acttcgtctt cagcaaggta gtagttttta agcatagaaa ttaacattat tatatttatc
     5101 tttatgcaga ataaggagtt gcctaaaaaa aacaactcct tattagcttt aagaaacaat
     5161 ttctatttta tctgttgctc cccatttttc aactttatta ttttctcctg atgctaattc
     5221 tacataaagt ccatttacct tatttccttc taaaattata tcactattag aagtactttt
     5281 ccaactggag tctatttcat ctcctacatt catagcttct ggttttgtag tctttattct
     5341 atagtaaact ttattaccat tgtatccagt tacactaata gttactttag aattttcatc
     5401 aacagaaaca gttgatttta ttccatttgc tactatttta aaaccatcat ctgtcgcacc
     5461 agctcttccc catcttgaaa ctttctttct tgtataatca atttcaacaa cttctatata
     5521 tgatccattc ttagcattta cttttaatgg ttccgtatct tcaaccaagt cccaatcatt
     5581 tatgttaatt ctatcaccaa ccctcatagc tgatggttta tatgacaaaa ctctataata
     5641 aattttataa tttgaatttt taggtttctg aggtacagtt aacataattt gattattctc
     5701 aagaggagaa catgcactta tctttggaga taatcctgct gctgtatcac tatcatcatc
     5761 tttatctgta ttattagtgt taggtttatt gccacttatt cctggttctt tatttccact
     5821 aacagtatca atctttcccg aatttcttat attagtactt gaagaatcat tatctatctt
     5881 tgttatagtt ccatcatttc ttatatttac atcttttgca gaactaaata ttgttacaat
     5941 ccatatttgt ccattatttt cattttcaat cttgcaacca ttatcatcag aaatgtccat
     6001 ttgatttata tatcctgaat tagttaaagt tccatctttt atatctttaa gtatcaatgt
     6061 tttacttata tcacctgtat tattcaactc tccatttggc atatttatag ttattgactg
     6121 actatattct ccatataaat caacctttat agctttttca gtctctaatt taaaagattc
     6181 acttattttt ttatcaggtc taaactttat aatatcatta gcatccactt tctttaaagc
     6241 agaatccaat tcttcaattg tttcaactgt atatttagtt tcttcttgca tatccaatat
     6301 atctttatag acattttcat ttcctattcc accaacctga gtaactgtac taaatatctt
     6361 agtatttagt acatctttct gaatagaatc aattttattt cctactaata ccactggaga
     6421 attgtttttt gcagccaaca ctccaactga tagtgaatct attaaatcac tttgtttttt
     6481 cattccatcc ttggttatat atgcatttct taaatctgtt tctttataaa attcttttat
     6541 aacattagca tttgtttcat ttctattatt tcctgatatc ctcttagaat ttggtaaatt
     6601 tttttctatt tcattagaaa tggaatttaa tcctccaata atataagaat tttttatatt
     6661 ttgggctttt ataaatttat tagctacttc aactccaagg tttgaatctg aaagtataat
     6721 tggcatgtaa ttttgagctg ctattgaacc aacacttaca gcatctgcta atcccttttc
     6781 tccatttact acaattatat cagaaatatc tttttcatta ttcaattttt cagccaattt
     6841 taaggatgtt tcatatctat cattcccaaa aattctttca actgttattt tctctgattt
     6901 taattcattt tctacattta cacctagtgc atttgaacct cctactaaaa atacattttt
     6961 tactccaagc ctttttagtt ctgattttgt cctaaaatcc aattctttac ttccagatag
     7021 taaaataggt gcatctttca atttagcaaa tggagttgca gataatgcat ctgctattga
     7081 gttatcattc actaatacag cattatcaga tcttaaccaa ccgttttggc ttatttttat
     7141 tgctgtttcc catctacctg aacctattaa actgtcttta ttagatgcat ttaccacatt
     7201 actaggtaaa acaattgata aaaatgctgc tgttaataaa gatattattt tttttgatat
     7261 ttgcattaat tatctcctcc taatttaaat ataattttat ggagcatgta catactgtgt
     7321 tttatttaat tctaaatcat caaaatgaga ttttactgaa tggtatccta tagcataatg
     7381 tccactttca ggattccatt tcttatatgc attcatcgca cttaataact tctcctcttg
     7441 actaggtaat accttccaga cttctacatt tggattaact tttttaaaat ccttatagtc
     7501 atatggttct acataaaatc ttaaatcttt tatttttcct tctctatata actctagtgc
     7561 agcttctcct aatgctctat gatcttcatg tattccacta gctttaaacg gagtaacagt
     7621 ttttactttt gcttctggat attcttctaa gtatttaagt attatttcct ttgccttttc
     7681 tttgcttaac tcaccatcat gagctctatt atcttctata tgtatattac tttcttttac
     7741 acccattgct tcacagctat ccttaaattc ttcatctcta tcttttgaaa attcactttc
     7801 tgataaagag tatttatgaa catctttaat atgataagaa catgaccctc catcatccaa
     7861 aacttttctt attatagatt tactaccatc tgtgcacaat atgacatgtg tatcactttt
     7921 ttcaacatgt tcaattatag cacttcccat acttaaggtt tcatcatctt gatgtggtgt
     7981 aaaaaacaaa gttgcatcta ccactactac atcctcctac tttataaaat gttataattt
     8041 acaataaata gcataattat agaaaaagat tcctgtatta attaaggaat cttctccaca
     8101 gtataagtta ttaccaacct agtattttag ctatcttttg tattactgtc aattcaactc
     8161 cattacctat ttgagttact tctgcaccaa gatttaattt tttactaagt gcattctttt
     8221 gtgagtctgt tatatcagtt ttagctagaa gtattggacc tttacctgct gctagaggtc
     8281 ctgctgccaa tgcatctact agcataccat tatttccata tccatctttt gctatatata
     8341 gtttatctaa tttgccataa tatgtttcta taactttagc attagtatcg tgtcttgttt
     8401 ctccttcaac tctttgaact tttctatctt tatcaatatc tgcaagttga ttttcaattt
     8461 ggctagatac attattagag ccaccaacta tgccaatctc ttttccatcc atcaatttga
     8521 tagcatctgc tgatagatca ttccaaccat ttactattat aggagcctta agttcagcag
     8581 ctttagcagc tatactcata gcatcagcct ccccttttgc gcctactaca aatgcatctt
     8641 taaaagaccc catagctttt gctactgcta cagaagtcat atgtctatca tctccagcta
     8701 gtctttcaac attcttagtt actgattcta attgcttttt agctgtattt gatactgctg
     8761 attctccacc tactatatat attttagctg atgggctttt cttaattata tcttttatat
     8821 aatctaaagt tacttttggt atttcattat cagatgctaa taatataggt gcttttttag
     8881 attgagcaag tggtgtagct gctaatccgt caactagttt atttgaatta actataacta
     8941 tattgtcagt atattttgtt tgttttgcta tagccactgc agttgcatat ctatcatctc
     9001 cagctatcaa tttaaccttt gaattacctt ctaatgcagc tttggcatct tttaaatctt
     9061 tttcattggt actttctaca gtaagctctg ttgcttttcc agatgcatca gtatacttaa
     9121 tagtaaattt atattttcct gtttctgaat cttttgctga aatatcacta attgactttg
     9181 atgtaccatt tgctaccata ttacctttaa cagtcaatct atatccatca tataaatctt
     9241 gtggtgaaac tgtatttagg tcactatttt ttatagttat ttcggccaat tggacatcat
     9301 caatattttt agctcctgct acacctagtt tatcgatttt atttactcta aatccttctg
     9361 cagaagggtc taaatttgtt ccagttgaat caactggatt tgctgttaaa tcaacttttt
     9421 catcaccaat acctacatat atagttttag aagttactgt ttctgtttta tctcttaaag
     9481 ttataactaa cttatcttta gcactatcat atgaagcttt tacatctgca actttataaa
     9541 ttccattggt ttcagtttta gctttttctg ttatagaatt agcctcagcg gaaaggtctg
     9601 ctgaattttc atatttttca gttgcttctg caactacttc atttgcacta tttgttatat
     9661 gtcctttatc tgttatagtt acaataagtt ctttattttc tcccaaagca tttactaatt
     9721 tctctaattc atctatatta gttattattt ttaattttga taaagtctca ccaactttta
     9781 tttcatatat tggttgtcca acttgagaac ctcctgtata cttttgagac attaaacctc
     9841 taactttcga aactaattct gtagcctctt tcttagttat tgtttctttt tttacctgtg
     9901 tagtttctgc agcaaaaact ggagctactg atgtacttat cattgcagca gccataatta
     9961 cagaaagatt ttttttattc atggtatttt ccccctaaaa aattcaaatt tttattattt
    10021 tttccttatt taccaattat aatatatatt tgatattatt tcaataaatg agacttattg
    10081 taataattaa ccaaaagata taaacttttt cttatatttt atttttttaa tatttacgta
    10141 tacaaaaaag taacaaaatt gtaatttttt ttaataatcc agtttataac taatagactt
    10201 ttcatcttct ttatagtata atattaaatg gaagaaagaa atttcaagca tctctttttt
    10261 ccgtatttct cacatagaaa aagaggctga ttatcagcct ctttttccta tacagtttaa
    10321 atttcttaat taaatttata taaatactgt atagtcgtct ctcttatatt tttatttaat
    10381 gattcaaaca tctcataacc ttccatagca tattctttga aaggatcttt ttgagcatat
    10441 gatttaaatc ctacatattg ttttaattgc tccatagcat ctatatgttc tacccaatat
    10501 tgatcaacta cttttaaaag aacaattttt tccatttcgg ttattttgtc aaggcctact
    10561 accatctttt ttaaatcata aactcttttt gatatttcat aagtactatc aattatatcc
    10621 tcaatacttt tattatctaa gtcaggtatt agtaaggtat cagctggcat aaatgtatta
    10681 taaagatgct taaaatatcc atagtaatcc ctctttcttt ttgttaaata ttcagcagca
    10741 gcttctttta ttatatcttt aaccatgttt tgaatatcta cttgtatatc ttcatcattt
    10801 agaactttat ttctttcgtt atagattact tttctttgtt catttatagt atcatcatat
    10861 ttaagaacat ttttccttaa ttcaaaattt ttaccttcca cccctttttg agctctctct
    10921 attgcctttg taagagattt actttcaata gaattatttt tatttgaatt tggtcgtttc
    10981 attagtttct ctatactttt tccaccatac aatttgatta catcatcttc aagactaaca
    11041 aaaaaccttg atgttcctgg atcaccttga cgtccagaac gccctctaag ctgattatcg
    11101 attcttctag attcatgtct ctcagttcct attacataaa gtcctcctaa ttcctttact
    11161 ctactttctt cttctttatc acctgctcct aaagcaatat ctgttcctct acctgccata
    11221 ttagttgcaa tagttactga acccaactta ccagcctttg acactatcaa tgcttcttta
    11281 tcattttcct tagcatttag aacttgatgt tttattcctt ctttcttaag cataccagat
    11341 aacatttctg atttctcaac agaaacagta ccaataagaa ttggctgttt tgttttatgt
    11401 atccttatta cttcttctac aacagcttga tacttttctt tctctgttct aaacacttta
    11461 tcatgaagat ctgctcttat aattggtttg ttagtaggaa tttgtacaac atttaattta
    11521 taaattgatt caaattctcc ttcttcagtt ttagcagtcc ctgtcatacc agaaagttta
    11581 ttatataatc taaagaaatt ctggaatgta attgtagcca tagttttaga ttcatctttt
    11641 atttctaccc cttcttttgc ttctatagct tggtgtaagc catctgtata tcttcttcca
    11701 tccattactc ttcctgtaaa ttcatcaact atcattactt cttgattaac aacaacataa
    11761 tcaacatctt tttccataag tttgtgaccc ctaagagcct gatttatatg atgatataat
    11821 tctatatttt ctatatctgt aagattttta atcccaaaaa atgattctgc cttatcaata
    11881 ccactttcct ttagagctac ggtcttatct tttctatcta attcataatc ttcttctttt
    11941 attgttttta taaatgaatt tgctaattca taaagttttg aatcttcatc accctctcca
    12001 gcaattataa gtggagttct agcttcatct ataagaattg agtcaacctc atcaactatg
    12061 gcaaaattca actctctttg tactttatca aactttttag ttaccatatt atcttttaaa
    12121 taatcaaatc caaattcatt atttgtacca taagtaatat cacaattgta ttgaactttt
    12181 ctaacttcag gtataagatc tgcaataatt actcctacag acatacctaa aaactcatac
    12241 actggttgca ttagctcttt atcacgttgt gctagatagt catttactgt aacaacatgc
    12301 acacctttac ctgttaaagc atttagataa acaggtgcaa cttctaccaa agttttacct
    12361 tcaccagttt tcatttctgc tatttttcct tgatgaatta caatgcctcc aattaattga
    12421 accctatatt gacgcattcc taaaactctt tttgaagctt ctcttgccac tgcaaatgct
    12481 tctggtaata aactatctaa agtttctcct ttttttaatc tatctctaaa taggtctgtc
    12541 atttttttta attcgtcatc agacatattt tctatatctt tttctaaaga ttctatttta
    12601 tcaactatca cattaagcct tcttatttcc tgctcatctg ctttatccaa tattgtatcc
    12661 aaaactgaca tatttaattt acctcctttt ttatcataca attactatta taccttaaaa
    12721 ttatagaata gtagacaatt atttttattt atagtataat aatcatactt aaataaatta
    12781 catctttaat tatttaggta tttctcacaa aaaaaggaac tatttttagt tcctttttta
    12841 atttcttttt ataaaaaaga gaacttcttc aaagaagttc cctcttattt atagtttaat
    12901 tttatcttat aattctaata aatcttttat tctcttaata actgaatctg ctattccttt
    12961 accaacttga gtcaatttct tagaatcatc agaatttgta actttactta ttgctactga
    13021 ttggtcagaa gataaactat cagttgctaa aactattgga gcttcattgt gacctgctac
    13081 tgctgctatt gctaacgcat ctactaattg atcttctttt gtagaaccat ctttagcaac
    13141 atagaagttt ttaacacctg tgctaattac actgtcacta ttatcttttt caaaataagt
    13201 ttttataact tctgcattag tatcttgtct atcatctcca ctaactcttt ctggactctt
    13261 acctgtagca tcatctatag catcttccat gtcttcagaa acactagatt ttccacctat
    13321 tatatctaca taagcacttc ctataaagtc ttttgcatca gttgataaat cttttccttt
    13381 tccatcaact actactattg gcatttcttt attagaagca actgatgcta tactcattgc
    13441 atctgctaat ccagtaccac caactacaaa tactttatta tggtttaaac ctatttcatc
    13501 agcaatttct aaagatgtag catatctatc atcaccagat aatctttcaa cttttaatcc
    13561 catatctttt aattcatttg ctacttcttt agatacagaa ttttccccac cagctatata
    13621 aacagtcttt ttagatgtta taccagtttt atcatctaat cccattactc tctttatttc
    13681 agattttact gatgaatcta acttatcttt tgaagttaat aataatggag catctttaac
    13741 agcagctaaa ggtgaagcta ctagaccatc tactattgat tgtgatccta ctaatactac
    13801 ctcattagct tctacataat ctcctttaat tcctccatct gcattataag agtctttact
    13861 tatttctatt gcagtctcta ttctatcatc accagctaat acagaattat ttgaataaga
    13921 attattataa gttcttaatt catctaaagc tgattttaag ttattcttat tagtagatct
    13981 taatgttatt atagcaggag tatctactct atcacttgta ggtaattctg gcttatagtt
    14041 agatgatgca tatgttgaag ctgtatttaa tcttttacct gttggataaa gaactacctc
    14101 ataataatca ttgtcagttt tttcaaaatc acttgctgtt actgtcttat atgcatcttc
    14161 taattcagta tctttaaata catatttttt agctaaagct tctgcttttt cttttgcatc
    14221 tccagatatt gtaactgttt catctgatgc tgctataact gttacatttg attcaactgc
    14281 tccaactttt tctccagttt tttcaaaact taattttgat gtaccatcag tagtattaac
    14341 cgttataact ggtttactaa aatcatatat ctctttagta ccatcaagag atgctactgc
    14401 tgtatctaaa gtaggtgcta ctgctgcttg tgtatcatat tttaatttaa ttgcaccagc
    14461 tactgcatca gcatatgttg ctgtaatagc tactgttcct gtagcattat taccagtctt
    14521 atcagtaaat tttgaagttt tgactgctgc ctttgtagct gctaaaactt catctttagt
    14581 agctccatct acataagctg acgtagttgt atcatatttt tgtaacttta atttagcatc
    14641 attttctatt gaatttccat cttgatctac aatcttaact gttgttgcag taccaacttt
    14701 agtaactaca gcattactat caagttcctt taataatgta tcatcatatt ttttttctgt
    14761 tccatctgta atttttacag atatttttgc atattctcca gcttttaaat ctttaatttc
    14821 atctttaaca taacttattt gacttgcttc taaacttgat atagataccc cttttggatt
    14881 tccttttttg tcaaaaaagt taactgttat gcctgttaca ccatctgcaa catatttttt
    14941 aagtattctt tctatatcac tatacttact actagataca gtggtatttc catttggaaa
    15001 actatcctct gctgcagcga aaactggtgc tgctgatccg attactgtaa cggcagacat
    15061 tacaactgcc aaatttttct tattcattcc ttaaattcct cccaacatta tattaattac
    15121 atctctttaa ttatgtacat taaaattcca tgttcatata aaccaaatat ttctcacact
    15181 cttatgatag actatataaa atagaatttc aataatttat gcaaaattaa tagaattttc
    15241 tttcaacatt tttccccttt tttttgctat ttcatatttt ttaaattaaa ttttcaattt
    15301 taattttatt aatacattta tttatctgat ttaatcaata tatattaata ttttctacca
    15361 taaaatatta atatatactc taactattta aaaattccat tgtagcatta acttctcttt
    15421 gattagaaac atacattcca aagccaaaac tataaaactc ttcctttaac atattatcat
    15481 tatacttaga agacttcttc cataaatcaa atacagactt agcaatctct tttgcatctt
    15541 tagatgtata tacagactta acattcatag gtaaatatgc aatattttca tcagttctca
    15601 ttccaaagcc agaaaatact tcagctgcat ttttcccatc tatttcatga gcaaatatct
    15661 tcttctcatc catatactta gaccatgcat ttgccattcc agctaaagaa tctaattctc
    15721 ttaaactttt cttacctttt tcttttctat atgaattaac tagattatac atttcattag
    15781 ataccatagc cttaaactca taactattag ggtcttttgt acttgtgttt ataattactt
    15841 tgacatttac attaatctca cacttttgcc caaaattatc aaaagcactt acatttaaaa
    15901 catacgttcc tgccttactg gtatccacag ttccctcaat attaggcttc aaattatttc
    15961 cataataatc agtggcaact atattcaaca tactattatc aaacttatcc cctacagaaa
    16021 tctctacatc attagcagtg attgtaggca ttgtattttt atcagatgca gaactaatac
    16081 attggtcagc aaccttctta tccattccac ctaaagaagt aacttcatca gcacctctaa
    16141 gcacactctt atcacttcca tcattaacaa gtactatagg tttgtcctta gccaacggag
    16201 aaccagccac agcatcaact aacttatacc cttgagaaac ataaaacttc tttgttcctt
    16261 tataaaagcg ttgtataacc ttcttattag tttcaaatct atctttacca gcaatcctaa
    16321 cagaattagt cttactcaca agttcattac tcattatctc ctctgaccca agagaataac
    16381 attgaacgcc atcaacctta aatggtatac tctttccatc agtaagtata acaggaactc
    16441 catctctagc agctacagaa gatacactca ttgcatcagc ctcacctgtg tatccattta
    16501 caagaaaaac cttatcacca tcatttactg gtttaatagc tgaaatctcc ttagctataa
    16561 gatagctagt ctttattcta tcttccccac caattctttt aacttcaatc cctttattct
    16621 taagttgatt ttctacagat ttacctattg tatcctctgt acctataaca taagcattct
    16681 taacatcctt tagtcttttt tcaacatctg ttggtatctt atctctttgc actaacatta
    16741 taggagcttt tacaactcca gataatccac ttgcacttaa tccatctact atagaattat
    16801 cagtatttac taatatgact gtatcatata tttgtttatc tgctatcaat gcagaagtct
    16861 catatctatc tttcccttgt atcttttcta tactcgaaag tgcattgata tttggtgctc
    16921 ctgccattat taatgatatt gtcaatccta ttgataatac tctcttattt accttcatcg
    16981 aactcctcct actaattttt ttatataatt cagctaatca taaattacac cctcagtagt
    17041 gattccctca aaatttataa actactaaaa caaaaaacac ctaaaatacc ataacctgta
    17101 aagcaataat aaaacagatt acaaaaagta ctttagatgt gattttcatt ttataatatc
    17161 agtaaattaa caaaaaaaca agatttatgg aattttttac aagctacctt cattgccatt
    17221 taaaaactct tgagtagcat gcacttgacc atcagataat atataaagcc caaatccagt
    17281 actataaaac tcatcactaa gcatgtttgc attgtattct ggagatttct tccacacttc
    17341 aaatatagcc tttgccaagt cttttgcatc ttgagttgtt tgaacactct tagaatctat
    17401 ataaatatat gctatatttt cctcacttct cattccaaac tcagaaaata cttgtggagc
    17461 atttttgcca tctatataat gttcaaaaac tttgttatcc atcatatact tagaccatgc
    17521 atttgccata ccttctagct tactagatac taccaaaggc tctttacctt tttcttttct
    17581 ataggaattg attaagttat acatttcagt agataccatt tttttaaatt ctgggctgtt
    17641 gtaatcatga gatttatcat ccaacacttt tatttctact ctttttcctg tactttttcc
    17701 ccattcgtct gtagctttta atgtcaatat gtatgtacct actctatttg tatctataaa
    17761 cccatcaact tctattggta ataccttccc agtgttatct ttagctacta tatttaactt
    17821 gccagtgtca aacttttcac ccttatatac ctctgtactt ccaactgtaa ttgctggggg
    17881 ttgtccattt gactttgaag cacttataca ttgttgtatt accttctcat ctatttcacc
    17941 tacagaggtt atattcttag ctccttctaa tacagtttta tcgcttccat catttactag
    18001 taccattgga gagtttttag taagtggtgc agctgctatt gcatctacta attggtatcc
    18061 atctgataca tagaatccat ctgcactatt aaaaaagtag tctattacgt ttttgttagt
    18121 ttcaaatcgg tcagttcctt ctattcttac tgagtttgta tttttaacta gaggattact
    18181 cattatctcc tctgaaccta tacaatatga ttgaatattt tttacatcaa aaggcacact
    18241 ctttccatct gtaagtatga ttggagctcc atctctagta gctactgatg atacactcat
    18301 tgcatctgct tctccactat atgcattagt tagtagtact ttatcaactt tttttatagt
    18361 tgctatttct ttagctatta aatagcttgt ttttagtcta tcttctccac caattctttt
    18421 gacttctata tttttagaat ctagttcttt ttctactgat ttacttatag tatcttctgt
    18481 acctattata tatgcttttt tgatattttt tagacatcta tttgtgtctg ctggtatctt
    18541 attttgttgt gtaaatagta ttggagcttt tgtagctccc gataatccac ttgcacttaa
    18601 tccatctgca agtgattttt ctgtatttac taaaattact gtttcataag tctgtttatc
    18661 agctatcttt gctgctgttt catatctatc tttaccgtat attttttcta ctactgatga
    18721 tgcatttaca cttttgaagt ttactaagat taatgatact gctagaccta atgataatat
    18781 ttttttgttc attttcatat ccccctgatg atttttatat tttatattta tattgtgatt
    18841 attttccatt tattttctct ttattttcca tatattatag agtatatatg atatgaatat
    18901 atctcgtcta tatattagtg ttttatttta ttaaatttaa aaaattattt ttaacttctt
    18961 tttaaacata ttcaaaattg aatttatatt tatatatata aaagatatct gctttattta
    19021 aaattaaact taatatagct caaattatta tacattttaa cctcaatatt attagattgt
    19081 taagataatg aaaataaaat aagaatgcat ttaaatatat aactttatat ctaaatacat
    19141 tcttattttc tatttactta atatttattt ttcaattaaa ctcttaatct tgtctattac
    19201 agatgaggct attcctccac ctacttcaaa caactttgat gaatttttac cttttaatga
    19261 cgattcttgt gatttatcta acgtatttgt agcaagtata actggtgaat tgtttttagc
    19321 tgctaatggt ccaactgcta aagcatctat aagtttatct tgatttgcaa ctacaactcc
    19381 atctagctta gattgagagt agaatttatc tattactttg gcattcgtct cttgtctagt
    19441 ttgacctgcc accctattgt ttgatatatc tttgtttgct atttttccaa cttgttcaat
    19501 agccttgtta gatatttttt ctactccacc aataaaatat atatttgaca ccttatttga
    19561 tttaataaat tgttcagctt cattatctac accatctttt tgtgtaagta ctattggagt
    19621 tttttcttta ccagccagtg atgctattga taatgaatca gcttctccat ttcctgctcc
    19681 tatatacaac ttgcttacat tattcgtctt atctatttct tttgctatat taatagaagt
    19741 ttgatatctt gtatctccac ctattctttg tactgatatt tttgaattta cagctttgat
    19801 agcttcaaca actgaatttg gcattgagtt gtttccacct atcacaacta cttttgatgg
    19861 atttagcctt ttcaattctt ctacagtttt ttgaggaagt ttatcatttg atgataaaag
    19921 aatcggtgag ttttttacac tagcaagtgg tgtagctgta agcccatcca ccatactttt
    19981 atcttctcca tttactatta caactgtatc tgattttgtc caaccacttt gagatatttt
    20041 gatagctgtc tcaaatctat catcaccttt taattttttg actgtgacat tagatgtaga
    20101 atcacttgaa tttcctcctc cagagcttcc accactagat gagccgcctg aattacttcc
    20161 tccaccatta tttccaccac taccattatc tggtggtaat tcatccggtt ttgataattc
    20221 attttcaact atgtattcta aactttttgg aagtgtaact tcaacatatt tgtttattgt
    20281 aacttttctt cccttagttg ttttttgtgc taatccaagt tttaatatta catttgaacc
    20341 actttcttgt gtttctttac taacaataga atagtttatc ctaatattgt ttaataaata
    20401 ttcaagtgct tcacttttct ctaccttata cttttcaggg tcatttttac tagaaccagt
    20461 tcccccaatc ggttttactt cactctcatt aataagggta aatattaatc cttcatcaga
    20521 gcttatattt tcataatcca ctgttttgtt gttatatgtt acaattttta aagctggttt
    20581 atcatttgtt ccttttccta aaactttata gtcagcattt gagttacttc cagatttttt
    20641 tacagatatt cttcctatat tcaatctatt tccgtctagt aagtcttcat ttgatgtaac
    20701 atatatatct aatgtagttg tattacttga tttgttgtat tctgctctac aagtagcatt
    20761 tgcatttcca ctttttatct gagattttat attatcagac caagtaatct tttctttctg
    20821 ctcattatct actacttcaa cctcaccttg tacttttata ctggcttgaa atgaatatac
    20881 tatgttttct actgagtcca cagatatatc tatagtattt actttctcat acgtttttgt
    20941 tccttgtaaa actggagctg catatgcaca tgtagcacta ccagttacag ctactgccat
    21001 agttgatact gctatagttc ttataaaaga ttttctttta ctcattaaca cccgtcctta
    21061 tctatcatta actaagtgga taaatttact tatccaccta gcttttatat ttaatttata
    21121 aattacccta aatttatgat tacatttata tactttactt ttaatatcta ctattttaaa
    21181 actattctag tacctaatct tattagatat acaatgttag tgttagtatt tttacgtttt
    21241 tagagttcca cctactattt atctctaaca atattaccct ttactttatt gcttacagtt
    21301 ataaccttct tattactgat atcaatagaa tttcttgtag cttttctagc tttactatta
    21361 ctatcagtaa gacttattgt ctttaactca tcataattct tagctcctac aggtacatac
    21421 attgtatcac ttacaaatct ttgacctact ggagtatctc cttgaaggtc attgtatcta
    21481 tatagttcag ccttaacctt atcacttaaa gaatcaaagt tttcttttgg catatagtaa
    21541 acccatgtac ctttactgat ttcacctagt tcaccttttt ctggaacttc tgcaaataaa
    21601 agtgcttctc cagatatagt cttattgttt ttatctataa gtagtggtaa attaaatgct
    21661 ggatttccac tgtattcacc tgtaataact attgaacccg ctggaatctt acctgtttca
    21721 tcttcacttg tgctagagtc atcactttga tatacataat cttctgctaa ttttccaatt
    21781 ccattagtac gagtttcgtc tgcattattc attgtgccaa tttctatatt atcacctgga
    21841 ggtcctatta tatctaattc tgcaacttct atattagact tattatttga gattagtttt
    21901 acatatctag cttggtatgt ccaaagttta ccatctttat taaatgtaac tctagcttca
    21961 ttatcttggt cttttcctcc accaactaaa tcttgtccat attcaaaagt tccttgactt
    22021 acatcttccc agttcttttt atcattactt acttgtattt tgtaattagt taaaggattg
    22081 tatgttgtgc tagtattctt tctaaagtta aataatcctt ttaatgagaa cttcctagct
    22141 tctgtctctg gttttgtata ttttagtcct actataggta atgtttcttt catgtctact
    22201 attatatatg gattttcctc tacatttata tcagggtctt tttgagggtc tttgttccac
    22261 tctgctttac ttattatctt tcctttatat gtagtatttg catcattatc ttttatatta
    22321 tcaatttcag gattttgtaa tggtccatgt gagtcatttt cagttctaac atcaccgtca
    22381 gaaacagtat ttgttatgaa tgaccatgct gatttattta ttttcccttc atctgataat
    22441 tttacagtac caacttcaaa ttcttctgtt ggatttaggt gataatcata tgcaactacc
    22501 ttatatgtaa atgctctgtt attaagagga gatatattat cagtaaattt agtcttacta
    22561 tttccttttg catccacaaa tccaactgtt ctgtatttta cctgtgttcc attttcacca
    22621 tctaaataat ttccatcact tcttattatt tcgtatccta gtatcttatc tttatcctta
    22681 tctacatcta ggtctataga tattttatta gatttaacta gagaaccact ttgtattcca
    22741 tcagaaaaac tagcacttac tttagtatct tttgccatac tagctatatt ttcatttaaa
    22801 atctttcttc tagcttcgtc atttacatat tgtatctttc ttgattcttt ttcgtagttc
    22861 ttactatcca ttatactgtt taattctggt gtaatttcta ctccccatgc cttaaagtaa
    22921 tctcttaaat ctttttcaac aacacttgat gccattgcta ctagcatttg gtcttttggc
    22981 aaactattaa ttgggtcact acttgaaagt gctctgtatt ttcttgaaat ctttgcatag
    23041 aatgtatcat tacttaaatc agcatctgag ttattcttta acattgaacc cgtaaaatca
    23101 tcatcataag ctagatgtaa ttgccataac attccaagcc tttcaaatac attgcttggt
    23161 atacctaagc tatttgaagt tacatgctca tatatcttat ccattgttga accttcaatt
    23221 cttgaatgtg tcttatcatc aaaggtctga gcaagtaaag caagtacatt gttactagtt
    23281 tcgctatatg tcatctttcc aatatctgta acatgtccta tctcatgacc tattccccat
    23341 ccaaataagt ttcctcctgt agcttttcca ctatcatcta tttggaaagg tactccattc
    23401 ataagccctg gaactgaacc atattctact cctacatgta atccagaagc atacataaat
    23461 gctccaataa acattctttg gtattttatg ttcatacgag attttggagc catatcattt
    23521 ttagttaagt taaattcatt atcatctaca gttccattac catctaaatc ttgtgtttta
    23581 aatactcctc tttctgagta aactaaatcc attatttgtt cccatgcaag catactatta
    23641 tataatctat ctacttgtgt atttaaatca gcatttcctt ttgatatccc ttgatataca
    23701 gctgttgcag gaacatttaa tgttacttta tcactttcaa tatctgtagt gtttaacaca
    23761 ccagtttttt ctgaatatcc atatgaatta ccaacatttg ttcctggata atatggttct
    23821 ccttttacag catctttata tctagctaat tcttctatat atgcttttat ctttttctta
    23881 gattcagaaa ctcccgcagg actcttatca tttattgttc catttagatt aaggtgtggt
    23941 atcttagtag ctcctgctac acgtactttt atatctcctc cacttggagt atcttcagcg
    24001 tatcttatgt ataaacttcc tccacattct atatctaaac tatgaagttt gcttaattga
    24061 attacatttt ttccagtgtt aagatttatt tctccactct tatatgcacc agattctcca
    24121 tagtactgtg tatatatcaa cttaggagtt tttccacttt tacttccaac atatactgtg
    24181 atttcatctc ctgctctagc agctactcct aatgcctgcc agtcattttg catacctaaa
    24241 ttattggaat ttacagtata ctgtccgttt ttatctttat attttcctaa tttatttatt
    24301 tcttgattta cagtagttac atcagtaagt gtggctctat cattatataa gtcttgagct
    24361 aattttaatt ctctctcaat aactgtctta tatggatgat attctccact agctttatct
    24421 ggagtattta atctatcttt taattcagtt attttttctt gagttacatc ttcttttaat
    24481 tcaacttgta ggtcatccat aaatagattt cttacatcat cttctatact gtcatactca
    24541 taaaacttaa gttctgatat attagactta tatgaatagg atgaacaaac acttacttgt
    24601 atcttggtag cagttattgg ttcttttaat ctaaccatta catacttatt attatttaca
    24661 gggtctgtct ttgtttcaaa aggaacatcc cccagattgt gtaattgacc attttcatcc
    24721 caatatcgta cctcaaagtc atttattgtc ttatcagaaa catcaaaacg aggtgtagcc
    24781 ataaaagtat ccatagtata agatttatca aaagttatta ttggtcctct attcgttccc
    24841 tcagctgcat tatatgttgg gaatgtccaa tgtgttgtaa agtccccatc tactacattg
    24901 aatttatcaa atggattttt atcactatat tcacttttgt catatcctgc tggataagta
    24961 acatctacaa tgtgatttgt tggtattcca aatccaccat tctctttagg agtattaatt
    25021 aatctatagt taggtgttgt tggtgcatcc atgctttcag ttgtacctat atatgtttta
    25081 gacattccac tagttcccaa gtggttagta gctgtcattc taacttcata tgttgtatta
    25141 tccttaagac cttctatctt tgaacttgtc ccacgaataa gcttatcatt gtattttatc
    25201 caagacccat tagagccttt ttctttatag tacaagtcaa aatctttagc ttgtttatga
    25261 tttttccagc ttacatcaag agatttaaat ttacctttaa catttatacc ttctggtggt
    25321 tctggttttg tgtctggaac aacttgaaat tctaatatac tatctgggct aaattgtata
    25381 ggtttatagt ttgaatctac attttctggt actccgtcta agttttctgg tgttgcatct
    25441 ttataaccac tagaccaatc tccatttaaa gattgtatgc taaccctata tagtccatat
    25501 gcttctatat ctctaacttc tattgtattt ttactggttt gtaatttttt agtagataat
    25561 acttttccct tactatcaat tttatctagt ataagttcgt atgctgatac atttacttca
    25621 ttattccaat aaaaagatac tttttcattt ccaacagcag tgctagtcga tattttattt
    25681 attttaggta tattcatctc tggctttggt atttctttat atacattgtt taaaaattca
    25741 acttttgata tttctgcaag atttctatcg tttgctgttg tagctgttat ttttatagtt
    25801 atctttttta ctgctacttg tttaccaaga tttattacta tatcactatt ttgaacatct
    25861 gcatatgttt ctaatgttga cttactatat gtagatgttg tagagttatc atctttactg
    25921 atattaccct cttcttttgg agtatcttca tctgaattag gtttatccat tgatgaattt
    25981 tctttatcat tatttttatc ttgattggta ttagagttta cttcttgttt atcgctattt
    26041 tcctgttgtt catcattatt ttcatcttta ttatttgagt tattatcagt ctggtttata
    26101 ttttcatctt tattatttac ttcttcttga tttacttctt cttgttcgtt atttacttcc
    26161 tcttgttgct cctcatcttt taagtttttc atatattctt tgttttcata aggaatctgt
    26221 tgaacattac catctttgtc agtaacctca actattcctt ttgctggagc agtaccttct
    26281 ggagctttta tattgatgac ttccatatta agtgcttctg ataaagctat atccattact
    26341 gctggttttt cctcagatat ttcatcattt tttgaagata ttgtaactcc attattttct
    26401 ccactaaata aattttctac attaccatta atatctacat cactatttcc ttttaattca
    26461 accttacttg tatctactat aggattggta gatactatac tttctaggct ttttgcatct
    26521 tcactttgtc ctaaattttt ctgtacataa tctaagtcaa caatatctac ctttccatct
    26581 ctatttaaat caaattctct tttatttgaa tctatattct ctaaaacctt attataatca
    26641 ctcttatcta ctttaccgtc tccatttaca tcacccatta agaacaattc ataatttaaa
    26701 ggtttatttt tattatctac acttgctcta ttgcttaata agactctttg tgaataactt
    26761 gatatatcaa tgtctttaat ttctttgttc ttaaatccat ctccagagac ttctacattg
    26821 tattttccaa caggtaaatt attaaataca atattataat atgatatatt ctcatcttta
    26881 ttttttattt cacttctact tgcagataat ttttttatag tatattctat attatctgtc
    26941 ccaacattta ctgttcctga atctttttct ccaagcttta ttattccact attgttacta
    27001 tcatctttta atcgtaactg catattagtc atactttctg aatcgctatt ttttattggc
    27061 agtgaaaaat ttaaatctac ttcaagactc ccccttaact tttctgtaac tgatttttca
    27121 actgccctta gcttcccctc taatataggt tctgcaaaaa cgtttagtgg tgcactacac
    27181 atagatactg ctagtacaca agatattgcc tttttcatga aataacaatc ctcccttaat
    27241 aaatatctaa tttttcatga atttaaatac tataagtttc agacaataaa aaaagacact
    27301 caaagagaat cttaattata aaataaaaag actttactct ttttagcaac tggctgatat
    27361 ggctttaaaa gctttaatcc ctatagtttt gcgtcattag atttccctaa ttttgccatt
    27421 taattttcac tactttctta cccattttat actatattta aactttttaa tacaattttt
    27481 ttagattaaa ttataaaaaa ataacattta tattaattta ttaatataaa tgttattttc
    27541 cattataatt taattattta tatagcttcc ttacaagcat aattttgtgt atattttctt
    27601 attctttata tcttccataa aattcatgtg tagcatatag attcttatct cctgttatag
    27661 ctataccaaa tcccattatt tcataccctt tatgcaacat gttagttcct tcttctggat
    27721 ttgtcttcca ctcattaaaa ataaagtttc ctatatcatc tgcatctcta tttgtatata
    27781 caggtttatc tggaatttta tatttaatta tattttcacc ttgaactgct atatatccag
    27841 gtttaatctc actccaatct aagtatttca aaaaagtaga atatgaattt tttccattta
    27901 tcgtatgaga taaagtcttc ttatttgcca ttaaattaga ccaatcattt gctaaaccct
    27961 ctaatctgct tgctacagat aacgctctta ctccattctc ttttctatac tgctttgtaa
    28021 gagcatatac tttacccttt attctctctt taaatgctct atcatttgga ttgtaatata
    28081 aaccttgttg atttggtgaa gcttgattta tacattgctt gataacattc tgatttatgc
    28141 ctcctagtac agtcaatttg tctgcaccac ttaaaatact cttgttactg ccattttcaa
    28201 ctaaaactac aggagcattt tttgctattg tagaggcagc caaagcgtct gttaattgta
    28261 agcctttact tagataaaat tctttactat ttttataaaa ctcttgtata actatcttat
    28321 tagtttcaaa tctatctgtg ccacccaatc taatagaatt tgtgctctta actaattcgt
    28381 cgctgattat ttcactagaa ccaagcgcat aactctttaa gcctgtcgtg tcaaatccca
    28441 cactatttcc atctgtaagt attataggta ctccatctct agcagctact ggtgatgcac
    28501 ttatcgcatc tgcttctcct gtaaatccat tggctataat tactttatct attggcttaa
    28561 tttctttgat tttttcagct atttttatac ttgtatcaaa tctattttca ccaaagattc
    28621 tttgtacaac cttctttttg gatagtaact gattctcaac atttttactt attgttgatt
    28681 ctgagcctat tatatacaca gtctttgctt tgtttaatct ttccatagtt gtgtttggaa
    28741 tctgattttg ttttgtcatc aaaataggcg catttaaggc tccagctaaa ccacttgcac
    28801 ttagaccatc tgctaagcta ttatctgagt tgattagtat tgctgtattg tagttttgct
    28861 tatctgctat ttttgctgct gtctcatatc tgtcattccc ttttatttgc tcaactgatg
    28921 tcaacgcatt taccatagga gtatttacca ttactaaaaa tagtgtaagt cctatagcta
    28981 atatcttttt actattcttc atattatttt ctacccccaa tgtacttttc atatttttta
    29041 ttagtactca tacttatttt taatcaaaag ttacaatttt gttaccatat tttatatatg
    29101 ataccatagt tatccataca actcaaacaa tatacctaaa gtttttattc tctataaatt
    29161 caaaacatta taatttattt tatttaatta agttataagt tcaaaataat ttatgttaat
    29221 aagatgttta atcattataa gaagtatctt tttataataa tttttgcaat atgaatttct
    29281 caacattata atcaaaaaat aaaataaggt atctcaatct aattctaaat gagacacctt
    29341 atttttatta ttttatcttg ttatttgtat attttataat atctacacta tgtcttacat
    29401 attaacgcat tgtataatca tatattatct aaacaaactt ttaatattaa ttcctatatc
    29461 ttattagttc ttagtaaata agtttatttt ttctgctatt ttttcaatag cgtttttagc
    29521 tattccttca cctatttgta ctaacttagt agcattctta agtcttaact caactacttc
    29581 ttcttgtgat ttagaaagtt cttctgtagt taatactatt ggagcctttg aactagctgc
    29641 aagtggtgct gctgtaagtg catcaactaa ttgtccattt ccacctacat aaccatcttt
    29701 tgctacatat gctttagttg cacctgcata gtatttattg attactttag cattagtatc
    29761 ttttctattt tctccagcta atctttcaac tttattattt aaatctatat caactaattt
    29821 gtcttcaact tcttttgata cagaattttc tccacccact atttctaatt cttttccttt
    29881 taataatttt tcagcatttg catctaattc gttacctgta actattattg gagcaccaaa
    29941 ttgagcagct ctagctgata tactcatagc atcagcttct ccatttccac ctactacaaa
    30001 agcagtctta aatgtatctt ttaattgttt tgctatttct aaagaagttt caaatctatc
    30061 ttctccagaa actctctcaa ctttacttac acctaattta gaaagttttt cttcagtttc
    30121 ttttgatact ttagattctc cacctactat atatattttc ttagaagata gattacttcc
    30181 aagtactctt aatatttcag cttctatttc tgatggtagt gaatcttttt tagataataa
    30241 tattggtgca tttttttgag aggcaagtgg tgctgatgct aatccatcaa ctatagcatc
    30301 ttctccaact aaaattatag cttctgcttc tccatctttg aatctctctt tactaacttc
    30361 tactgctgtt gtaaatcttg aatcaccaga taaaacttct acttttaatt cttttccacc
    30421 ttttacatca gttaatactt tgtgtaagtc tctaactaac ttatggtttt tacttgaaac
    30481 acttattgtt tctggctttt catttgcctt gcttaaaact atatcaaagc ttgatttttc
    30541 tggtttatct ttatcatcac tatcttcttg ttcttgagta aggattgctt cattttcaaa
    30601 tgttaaagca tatccatttt taactttatt aactatttct cttcctaatg tagttaatct
    30661 aattccatca aataattctt ctgctgatat gtcttgtaaa tctgcgtttt taactgttat
    30721 cgtatgtaat tcttttgaat ctatgtcact ataactcttt tcataacctt caaaagtacc
    30781 ttcttctgat gttatcactt ttgagaaatc taaatgttgg tctcctacat ttaatgtaat
    30841 aacatttgct gaatcttttg ctctttttac ttcaactgta tttgtagatt ttatagttgc
    30901 tgttaacttg ttatcagaat cttctgctaa agttgcattg taagcattaa ctgcatctac
    30961 tatttcttga gcagttttgt agttttcaat tttatagtca actacttttc cacctaacac
    31021 ttgatgtcct ttatcttgaa tagtaacttt taaactttgc ccttctgtta aagagtttat
    31081 cttattttgt aattgagttg cattagttaa gtttacattg tctactttta tatcgtaaac
    31141 tctatcattt acatttgctc ctgcttttgt atcttcaaac ttaacttcta atgcttttct
    31201 aacctcttcg attagtttag cagaatcttt cgcacttact gtgtaatttt tatcaacatt
    31261 ttctgtcaca gtattgtctg cgaatattgg tgctgctgca ctcataactg tagcacctgc
    31321 cattactatt gccatacttc tctttttgtt gcttaacata tttatgtcct ccgaatatta
    31381 ttttttattt tttatattcg tatttcccac acataaataa tattatatat aatattattt
    31441 ttcaatatat tttatgtgtt tacataattt actaacacaa acactaataa tatcttcttg
    31501 aaccccttcc ccctgcttag ctctctataa ttatgctcaa agatagaaat cctattttat
    31561 agtgattttc tactaaacaa tcatatacta acaaaagtat tcaaactcca tttttatata
    31621 ctactatgca ctaatttttg ctttactctt attacttaaa tatatatcac taaattaata
    31681 tctatctcca tatattaaaa tatataacat attataaaaa tcatcataaa ttagagttta
    31741 aaattaaaaa ttattctaat acatgtaatt gataaaaaat aaaatataag agtagctact
    31801 ttaaaataaa tgctcttata tgattagata tttttgcata attaaacgat aagaaatcaa
    31861 atttaatttt acataaatag aatatatatt tttatataat aattgaaaat aaaagaggat
    31921 acctcataat gataaaagaa tcactttgag ataccctctt acaaacaact aaatcaataa
    31981 ttttctatct ttcacactct tgatttgcaa ctgtacaaga agttttacaa gctgattgac
    32041 aagaagtttg acactctcca catccacctt ttgcagcact ttgctttaaa gtagcttgag
    32101 ataaagtttt tacatgttta ttttccattt tcaacgcctc catttctaat tgtttcctat
    32161 tgattatatc ataaaactac tttaatataa actctattta aagttgacac ccaatacgcc
    32221 tccaatgcct cctgctgctg atattaacaa taacttataa atcatcccta cttcaaatac
    32281 aaacttttct tgtgctaaaa acacagtcaa aaaaatacat actatgtaca aaagtcctac
    32341 taaaagccca tacataagtc ctttttcttt catgtgtttt gaagtgtaaa accctcctat
    32401 agctgcagaa atagtagtta taaatgatga gaccatagtt atattatctc caccaatatc
    32461 tgtaaatgtc aaaaataagt tatacactaa taaaacagcc aaagttatta tatatgcata
    32521 tcctagacct ttaaaaatat aattagattt tcccataaaa aaacctcctc tcaaattaat
    32581 atatattcga gaaaaggctt ttaaattact aatttagtct tcttctattt cttctttttt
    32641 agatttaaca gttcctatag cccatttttc aaatggaact ttagttctat ttggtcctaa
    32701 ttctaatatt acaacatcat cttcaacttt agctacatta gctactattc cacctattgt
    32761 aatgaccttg tctcctacac ttaggctact tctcatttct tttaattgtt tgtctttttt
    32821 cttttgaggt ctaatcatta ggaaataaaa gattgcgatt actactaccc atagacctat
    32881 actcataatt atttgttgct gtggcattaa tttttcactc ctttgtaaat tatactcttt
    32941 tgtaaatcat aataaatgtt tatataacaa tttactttct ctatctttga gtactattga
    33001 atattatata ttaaaatgat tataattaat agtgcaaaac ctatatttca tatccatatt
    33061 ttgcaaaaaa ttcatttcta aaatcaagta atctatcttc cattatcgct tgtcttattt
    33121 gtttcattaa atttaataaa aagtgaagat tatgagtagt tattaatctt gcacccagta
    33181 tttcatttgc ttttataagg tgtcttatat aagctcttga atagttttta caagcataac
    33241 aatcacattc agggtcaagt ggagtaaagt cttcagcata agttgcattt ctaactacta
    33301 ctttaccttg gctagtcatt gcagttccat ttctagcgat acgagtaggt aggacacaat
    33361 caaacatgtc tactcctctt ataacacctt ctactaagtc atctggacta ccaactccca
    33421 ttaggtatct tggtttatcc tttggcataa gtggagttgt atgctctaac acgtcataca
    33481 taagtggttt tggttctcca acactaagtc caccaatagc atatcctggt aaatcaatat
    33541 tagttatttc tttcgctgat tgttccctta agtctttata catcccacct tgtattatac
    33601 caaataaagc ttgcttgtcg gtattattat gggcatcttt acatcttttt aaccatcttg
    33661 ttgttctttc taaagaattt tttacatatt ctctatctga tgggtatggt gcacactcat
    33721 caaatgccat cattatgtct gaacccaatg cattttgtat ttccatagct ttttctggag
    33781 ttagaaaatg ttttgaacca tctaagtgtg acctaaattc tacaccttct tcttttatct
    33841 ttcttaaagg ccctagacta aatacctgga accctccact atctgtaagt attggtttat
    33901 cccaattcat aaacttatga agaccaccag ctctttttat aagttcatga cctggtctca
    33961 tatataaatg gtatgtgtta ctaagtatta tttgtgaacc aatctctttt aattcttctg
    34021 gtgtcataga ttttacagtt gcttgagttc caactggcat gaatattggt gtttcaataa
    34081 tcccatgtgg agtgtgtaat cttcctaacc ttgcaccact ctgcttacaa gtctttatca
    34141 attcgtatct tactgcgtac ataaagttct ccttaatcat atttatttct cattatttct
    34201 taagatatat ttgtaataag ctattcatat aatttattgt ctaaatagat ttatcttcgt
    34261 cttctttgtt ctgttgttcc ttaattgctt cttcatcatg aatactcatt atattaagag
    34321 cactcttgtc ttttgttact aaaaactttt ttatagcaaa tagtcctgct atagctataa
    34381 ctcctattaa aacatctatc ataccttcat ttttaggcag tagcaacatt tttctagcaa
    34441 ttgcgtaaag taatgcctca ataaatgctc ctggtatatg aagagatagc attactacaa
    34501 gttcaacacc tattactaaa agtaatattt gtgctaaaaa atcattaagc tgactgtatt
    34561 gtactggatt ttgaaaatca actatatatg ctccccacat aagtcttaga acatctatag
    34621 ttcctaaaaa tactgctatt agaactacta ctgctaaaac acgttcaaat ataaaagcaa
    34681 tctttaatat agggttattc tttttttgtt tttgtaaact atgcatctat tacctcccta
    34741 ctttataatc atagcatctc caaaactgaa gaatctatat ctttctttta ctgcctcatt
    34801 atatgcattt aaaacattat cctttccaca aatagcactt actaacatta ttagtgtaga
    34861 ctctggtaag tgaaaattag taataagttt atctaaaact ttaaatttat aacctggata
    34921 tataaatatt tctgtccatc cactagtttc tttcatcttt ccatcctcat tacaagctga
    34981 ttctatagtt ctacaactag tagtaccaac acatattaca ttttttccat tttcctttgc
    35041 tctatttatt ttgtcagctg cttcttggct aaccatataa tattctgaat gcatcttgtg
    35101 atttaaaaca tcctctactt ttactggtct aaaagttcct aagccaacat gtagagttac
    35161 aaaggctata tctacacctt tttcttttat tttatttaac aattcttctg taaaatgtaa
    35221 tcctgctgta ggtgctgctg ctgaaccatt atgctttgaa taaacagtct ggtatctttc
    35281 tttttcatct agtctagcag tgatatatgg aggtagtggc atatttccaa gttcatccaa
    35341 tatttcttca aaaattccat cataatggaa ttttataatt cttgaacctt catctgataa
    35401 atctacaact tctccaatta gctttccatt tccaaaagaa aatttagtcc ctacttttgc
    35461 tctttttcct ggttttacta aagcctgcca agtatcttcc tctgttcttt ttaataataa
    35521 aaactctatt ttacctccag tttcaagctt ctctcctatt aatctagctg gtataactct
    35581 agtattattt aatactaagc aatctcctgg atttaaatat tctattatat ctctaaatac
    35641 tttatgttca atattacctg tctctttatc taataccatc aatcttgagc tagctctatc
    35701 ttctatagga acttgagcta taagttcctg tggtaagtca aatttaaaat cacttgtttt
    35761 caatgttaaa cttcctctct taaaatttct ccagctacaa taatatcaaa aaaacaactt
    35821 attttccaca atattaccat aatctatcaa gaaattgcta tttttaacag acaccatatc
    35881 cacaatatat agatattttt ccacgatata catgccttat ccacaatata tagatacaat
    35941 acacacaatt tttatcgtgt ttatccacag tatctacaga tataattacc attttgtgga
    36001 tatgtttgaa tttttagcca ttttagaaat attttaattt aatgactctt ttattttata
    36061 aatactcgta taatctattt ttatttttca ttgggatatg gaatttttaa gtgttcatat
    36121 gctaatggca tagctactct tcctctagga cctctattta taaatcctaa ttgaagcaaa
    36181 tatggctcat atacatcttc tattgtattt ctatcttctc cgatagaagc tgccaatgta
    36241 tctaaaccca ctggtcctcc tctaaatttt tctattatag tcataagaag cttctcatct
    36301 acaaaatcga gacccaaact atcgactcct aaaagttcca gagcatcttt tgccacttta
    36361 tcagttattt ttccatcagc tctgacttgt gcaaaatctc taactctttt tagtaatcta
    36421 tttgctatcc ttggagtccc tcttgaacgt tttgcaagtt ctaatgctcc attagactgt
    36481 atctctgcat ctaagatgct tgaagaccta agtactattt ttgaaagttc atcaactgta
    36541 taatagtcca atttacaaat aacaccaaat ctatctctaa gtggatttgt aagcatacca
    36601 gctcttgtag tagctccaat aagtgtaaat ttaggaaggt caagtcttat gcttctagca
    36661 gatggccctt tccctattat tatatctagg caaaaatcct ccattgctgg atataaaact
    36721 tcttcaacac ttctattgat tctatgaatc tcatctataa ataaaacatc attttcattt
    36781 aaattcgtaa gtatggctgc caaatctcca gctctttcta ttgcaggtcc tgacgtaatt
    36841 cttaaattta cccccatctc attggctatt atacttgcta gtgtagtttt tccaagacct
    36901 ggtggtccat ataaaagcac atggtctaac tgctcatttc tcgatttagc tgcttctata
    36961 aatatactaa gttgttcttt ggatttttct tgacccaaat aatcttctaa agtttttggt
    37021 cttaaactgt tttctatatc tatatcttcc atcttcattg tagaagttat tatcctattt
    37081 tcatcttcaa aaccttgcat ttatacccct cctctcgtac ttttgaaaat ctactttata
    37141 tattttcaaa tttacataaa ctccttaaat attgttttaa aatccaaata ttataaaatc
    37201 caaatactat ttcaaggatt tactcattat atatgtaagt gctttcttta taatagcttc
    37261 tgtattcata ccatcttttt tacatttttg aactgcctct tttgattccg acaaagtata
    37321 gcctaaagct acaagtgcat ctacagactc atcttgtgat atcaaggtag gtttttgtga
    37381 taataacgtt ggttcaaact ctatgttatt tttgtctacc ttatccttaa gctctaagac
    37441 gattctttct gctgtctttt taccaacacc aggagctttg gataattttc caatgtcttc
    37501 acttaaaata tatgctccta actgtgctgg tgatgcaaat gatagtattg aaagcgccac
    37561 cttaggtcct attttagata cagatgttag taattcaaac atcttaagtt cttccctact
    37621 tacaaaacca caaagactca tatcatcttc tcttacaatt aatttagtat atatttttgt
    37681 ggcttctccc acttgtaaat tcattattgt attggatgat acatttattt tatatcctat
    37741 cccattattt tcaacaacta tactgtctat ataaatttct tctactgtac cttttatata
    37801 actatacata cttaccacct atattcttta gagttttttc aagtttgttt gaatgagcat
    37861 ggcaaatagc cacagctaaa gcatctgcaa catcatctgg ttttggaact ttttttagac
    37921 ttaaaaatga agttaccatc tgttgaactt gtgccttatc tgctctccca tatccaacta
    37981 caccttgttt tacttgaagt ggagtgtatt catatacagg cttcccatta tgagcacatg
    38041 caagcatagt aacacctcta gcttgtgcaa ctgttatagc tgtttttaca ttcttgttaa
    38101 agaataattc ttccattcca acttcatcta tattgtaatt ctttactatt gtatcaattc
    38161 ctttatacac aagttccaat cttctcgata tattcatatg ggcaggtgtt gtaactgctc
    38221 cataatcgat tgctttaaac ttgctatttt tgtattcaat tataccatat ccaactatgg
    38281 ctatacctgg gtcaatcccc aatattatca ttttttaaaa ttccccctaa aaataaaaga
    38341 aataccaaaa tgtatttctc ataaaaactt atgttctaaa tcattatatc ataaataata
    38401 ttaacaaatc ataaaatgct ctatgtaaaa tataaaatca aataaaaaag ttatatcaaa
    38461 gcaagatttt acctcaatat aacttttata tttattacaa ataaattatt gtatttttat
    38521 aggaattaaa tgtgactaca attttaatac taatctattt aatattaaat tatttattat
    38581 taatttactt agtatttaac ttaaaataat attaatttac tattaacttg caaagtcttc
    38641 taatcttgaa taggcctctt cttcagtatc cataactaca ccttttttga tttcatcttg
    38701 aactgtttta ggaagtgaat ctactcttat ttttgttttt tctactaatg ttttacttcc
    38761 gctatcatca tatttatata gtgctatatt tccattatca ttttcaatag tatatttacc
    38821 ttttatagta acatccattg cttgtacatc tttattatca ctatatttag attctaccaa
    38881 tatgatttca tttttatcca tactctttat ttttttagtt ggatattctt ttttaaaata
    38941 tgatactatt tctgacttcg tcttatctaa taatgactct ggaactattc ctgccattgt
    39001 aagtgttctc ttatagttag tttcattttc gccttcactt tgaagccaaa cttcacaatt
    39061 tttatttaat ccaaaagctt cttttgactt ttcattcata ttcgcactag tacttacaac
    39121 ttcaccagtt tgaactccat tgtgttttaa tcttgtccct atataaactc cgctacttaa
    39181 tacaaatact ccaaccagta ttaaggcaga tattttccaa ggaaataatt cttttttctt
    39241 cttttcaaac attgattacg cctccttttt ctattatttt ctccaagctg taagttttta
    39301 tacaatttag gtaaatttta gaagaaaata tatttatatt tcaaattact ctaagaatag
    39361 atattttcta aaaggtagtt ttctcttaat ggttatattc ttgtaattat atggacataa
    39421 tttataataa ataatcgttt aggaggtaat aactttggaa agaataaact gtaatgtaat
    39481 taactgttca cataacaaca gccatatatg ttatgcagat aaaattttta taaatggtga
    39541 aaaagctcac aatgacagac atacttgttg tagttcattt ttagatgaat ctatttatag
    39601 taatttatct agcaacgtta ataataaaaa ttgtgaggaa ttagtttgta ctgtaaaaaa
    39661 ttgtaaatac aacaaagatg gtagcttatg tacacttgat aatatattgg ttgctcctga
    39721 aactgataga gtaaaccttt atattgaaac atgttgctct agtttcgaat gtaaatagtc
    39781 atagtataaa tataagggtg tcaaatgtaa ttaaactaac atagtgtaga gtatctacaa
    39841 ctattagttt gattatactt gacaccctta tttattttaa ataaaatttc attattataa
    39901 ccatgtttat aatgttctaa ctgtaattaa atttgataaa tttctataaa ttggtataat
    39961 caatatgtaa attgaattta gtaaattaat cataaaaata aaatatcgag tatatagtgt
    40021 tatactaact agaaatgagg tgcttatgag tacattttat attcaatttc tttattttgt
    40081 aatttatagc tttataggat ggtgttgtga aactacatat tgttcagttt tacaaaaaca
    40141 atttgtaaat agaggttttt taaatggtcc attttgccct atatatggat ttggagcatt
    40201 agcaatagtt gctaccttga ctccttttgt acacaatatt cctttgttat ttttgtttag
    40261 tataattata actagcataa tggagtactg tacgagcttt attttagaaa aaatatttaa
    40321 tatgacttgg tgggattatt ctaaacataa atttaatatt catggaagag tttgtctgga
    40381 aaactcactt atgtttggtg tattatcatt gattgtaatg cttatagtac atcctattgt
    40441 ggttgacttt attaactcta tctctaaaaa tgtgctattt atatttgcta tatctataga
    40501 aatttatttt gttttagatt tagttatcac agtccataca attttacaat taaatggtaa
    40561 gttaaaacaa atcaatctta taattaagga attaaaagat aagaaagaat actacaaact
    40621 tattacacaa gaaactatag aaaacaaact tgaatcttta gttgaaaata gactggatac
    40681 tttggaagat aggtttgaaa tacttaaaga aaaagaaaat tatcgctatg ctaaaaatcg
    40741 tattgatgag ctaaaaaata aacttaatac tttgctgtct aaccacaaat tacttcatag
    40801 acgtataatc aaagcattcc ccaacataag ctctaataag catacagata ttttaaataa
    40861 aatcaaagaa aatattaaat caattaaaaa agataaataa taaaataaac acataagtta
    40921 aataataaaa atactaatac ataagattaa ggtttagaat attttttgta aaatactcta
    40981 aaccctaatt aatatactat tatttttaat cttaatttat aattttagtt tattttaatt
    41041 ttaactcagt aaatttaagc gtttaaactt caatacacta atatactacc ccatcaatat
    41101 ctcaactgat tctctaagtg tgctaatctc tcccacattt ccagggaaaa ttatatatgg
    41161 catattagga aatttgcttt ctttgcctgt catccatact ggtataccag gttttatctg
    41221 tcccattact gtagcttttt ttactcttag tgctttagta ccaacatcac tagaagtaat
    41281 tccaccctta gctataataa aatttggtct tacattaagc ataccaatta tactggtaac
    41341 tgcatctgat atttctacag aaatcataag ctgtttatct ttatcattcg tatccaagtc
    41401 aaaacgctct cgtctagtat aaacagcaac agttttacca ctgcttattt tttcctctac
    41461 aacttttata actcttttta cctcattttt tagaccattt tcttgaagaa ctaaatgttg
    41521 attaaattct ataaattcta tcggatattt acaattttta agttcttcta actgttgggt
    41581 agtcttattc acatgtgaac caacaagtat aattcctcca ttggtattac cttttgatac
    41641 taattcatcc ttagtaagta gttccttgtc actcactccg ccaagtactt ttgttatggc
    41701 agcagcgctt ctaaacataa attccttacc accatttact gctcgtataa acgcaattgt
    41761 aaataccttt acatctacat aatcggttgc atttacaata attttattga atccttcagc
    41821 ttttttaagt ttttctgtta tagcttcaat atttagctct ctaagttctt ttgacgaaat
    41881 atatatcata tcactagatt tgtactttcc atttgtcttt tcttcacacc actcacctat
    41941 atcagatgat ttatatccaa aagatttatc ttttgcaaat tcagtcattc ctgctggtac
    42001 aagtatttct ccttctttaa cataatgtac attatctata gtaaacctac caccttcttt
    42061 aaaaaatggc attattattt caccctgaaa taacttcccc gataatctct ctacttcgct
    42121 ttttaataaa ttagtttcaa gaggataatg tcctctcata gtagaatcac tacgacttat
    42181 gattataaaa tccttattaa ctttctttga tacatccaca atattctttg aaatttcttt
    42241 atgtgctttt gtagtttgag caacagtaaa tccccttgaa ttggtaagta taaaaaacat
    42301 actatttttc tcattaaagc cttgttccat actatcctta tcccaatcag tataaacaga
    42361 aatatcatga actgtttgta cacctgttgg gtcatcatct aatacaacaa tttttctatc
    42421 taaatgctta agctcatctg caagaagttt atttaatcta tcctcatcta cttgtttaaa
    42481 agtattaagt atatctatgc taattggttt tacttctctc atttaaaacg cccctttatg
    42541 taatttgtat agtctaaata taatttatat gatataaaca atagtttgtt atttatagac
    42601 ctggaagtgg tataattcca tttataacac taagcaataa aaccattata aatgctacaa
    42661 ctccagcaat ggttcctcca attgtccaag agtttaaagt atctttaact gataatccac
    42721 taaatcttga aagtacccag aatccagaat catttggaag ggagaaacca actcctccag
    42781 cacatatagc aagccctact aaaactggtg acattccagt tgccaaaatt gtaggtccta
    42841 aaattgatga tgttgttact aaggctacag tagcagaacc ttgagaaatt ctaagcaagg
    42901 cacttaataa gaatccaagt actacaactg atatactaag accagacata gtatcaacta
    42961 agaagttacc tataccactt gcatttataa cactgccaaa cgcaccacct gaccctgtta
    43021 tcaataatat tagccctgct gaatcagcag cttcaataac tacatctccc ataggtttat
    43081 ttatgtattt ctttaaaaat cctattgcca ccaatattcc aagaagtatt gctatgtttt
    43141 tatctcctat aaatgcaagt actccactca ttgtagaacc tttttctaag aacatactaa
    43201 atacatttga tataagtata agaagtattg ggaacaataa agaaaacata gaaattccaa
    43261 aacctggtac tttagaagaa tcatcatctt caatttttat gctatctttt ccctgctcta
    43321 aatttttttc tctatcattc tcatcaatag cataagctgg atatttttca aaacgttttc
    43381 cataaatcca tccacctata agagctgctg gaaaagcaac aattatagaa taaagtataa
    43441 aactacctac atttgcattt acatttgctg caactgccaa aggtcctggt gtaggtatta
    43501 caagtgcatg acctactata gtcccaactc ctaaggcaca tacaaaaacc attagagatt
    43561 tcttagttac tctcgaaaca tacttaatta taggcatcat tataacaaaa gctgcatcca
    43621 taaaaactgg aattgaaatt aaaaaacctg ctgtagttac tgctaaagca ctgttttttt
    43681 ctccagtctt agctaacaac ccctttgcaa tagattctgt tgctcttgat tctgaaagaa
    43741 tatttccaaa tattattcca agtccaatta caatacctat actacctaat gtatttccaa
    43801 atccagtcga tattgtggtt gatatttcat ttataggcat tctaaccata aagcctgtaa
    43861 ttatagatgt tatcagtaat gccaaaaacg gatttacttt aaatttaata atcgatacaa
    43921 gtaaaattcc tatagatata acaaatgcta tcaaaagtcc atatcctgta atcataatct
    43981 ttaccccttt atcattctac tttattattt atcaatttta tttttctttt actatgataa
    44041 cttaacctcg ctgtatttat ataatctaat cctaataata tgtatttaat tatattgata
    44101 tatttaaata ttaattatcg tgttttttta tttcaacacc tgccaacttt tcaaaatact
    44161 gtgctattcc actatggtca tcttccaaat gtccatgtac ttttaatgtc tgcataattt
    44221 ctaataattg acttgtcata ggtagtggta catctaaatt gtgtgctgtt tgcattacat
    44281 tcttaatatc tttcaaatta atagatattt ttccacctgg tttaaaatca cgattcataa
    44341 tcattggtat cttcacatcc aatattgtac taccagctag gcctcctctt attgccttat
    44401 atactttttc tgggtctgca cctgcttttg ctgcaagtac aaatgcctca gaaactgctg
    44461 ctatcgtcaa attaactatt acttggtttg taagttttgt aacacttcca ctaccattat
    44521 ctccaattaa aatagatgag cttcccatta tattaaaaaa tggctctact ttcttataaa
    44581 tttcttcctt accaccagcc ataaaagcaa gtgtaccatc aatcgctttt ggctcccctc
    44641 cacttactgg tgagtctata aattctaagc ccatatcttt taatttattt gcacacaata
    44701 ttgattctgt aggagtaact gaactcatat cgactactat acttccttct tttaaagttt
    44761 ttgccaatcc atccatccca aacaatatat cctgcacaat agttccattt ggaaggattg
    44821 taaatacaat atcacattct tgacccattt ctaaaacact tgcgtatttt gctccacatg
    44881 atataagttc atccacagca gatttattaa tgtcatacac caaaagatta catccatctt
    44941 ttaataaatt ttttgccatt ggctttccca ttatccctaa tccaataaat cccaatttca
    45001 ttttataacc ctccatatct tcacattttt gaaaacgttt tttatgtttt agagtatact
    45061 acataccagt ataccggtca atataaaata taaaagtatt tattttaaca ataaatagta
    45121 atttttaaag tcatttatta tatttagtca tcctatataa gttttaatac atactattgt
    45181 tttcaaatat aatatactac ttttacagtt ttgtatttta actaatttca attagtttta
    45241 attaatctgc ctttaatgtt aatcatgctt cacacttcaa taatttttta tattttttaa
    45301 tcttatctgc aatttaaatt gcaaaagatt gaccttattt tcactatgtt gtatgcttaa
    45361 aattatataa gtttttgtat ttttatattg tttagggggg tattattaag tatggctaca
    45421 tttaaaagat tacaagaaca agcatatgac tacttaaaag aattgatact atctggagaa
    45481 atgatagaaa atgaaatata ttctgaaaca aaacttgcta gtgaaatagg tatctctaga
    45541 acacctataa gagatgctct tcaaagactt agtcaagatg gatttataga tataatccca
    45601 agcaaaggat ttagaatcca tcaaattaca gcaaatgaaa ttgtagagat attccaaata
    45661 cgctctgcaa tagaaggatt ttgtactttt ttaattacaa gtcaatacaa agaagctaga
    45721 gcagtagaaa ctataagtaa attaaaacat cttttagata aacaaaaaga tattttatta
    45781 ggtgataaaa atttaaactc atttgcagaa tacgatacat tatttcacac aactatagtt
    45841 tcatatgctc aaaatactga atttgacaaa atgtttaaca attatatgta cagaataaaa
    45901 aaattagctc tcgattcatt gtcacatgaa ggaaggcttg aaacaacatt aaaagagcac
    45961 acagatattt ttaataatat tgcaaatgga tgtattgaag atatttacag aactactctc
    46021 attcatatgg aaactcctaa atacattaat ttagaagatt tttgtaacaa taagttattt
    46081 tagtattata ctttcattta ttaaaaatat atctatctca aattaatttc ctaatttata
    46141 aaaaatattc tataattata ttgtaactaa tacttattct aaggagtgat ttttttgaac
    46201 gaagatgttg taactaatga aactattgac aaagattatg ctattgaaga agttcgtatg
    46261 gcatgcaaac attttggtga tttatatttt tatttttcca aagtgttatt tgaagagttt
    46321 ggtgaagata aaactagcga aatattaaga aaagttttat ttgaaaggtc tgaagaaaga
    46381 gctattgcta tgagagagag agctcatgaa aatggagatg aattaatcgc agacaatatt
    46441 attagcacaa ctgatgttcc atttctagga tgggttcctg aatttgaaga gttacattgc
    46501 ccctatggag cttcttggct tttaagattt gaggaaaatc cttggtttaa aaaatttgct
    46561 tctctttact gtgatgttac agatactact gtagctgaag tatttacagg agatacgtcc
    46621 cataaaataa caaaaaacat tctatgggga gataaaagtt gtgaaagaat atatttccat
    46681 gatgacaagg tatctgaagg taaatatact tatggaaata aaaactgtaa ataaatgatt
    46741 taaattttct atattcatat actatatttt gtactttttc atataaatat tataactagt
    46801 cttttatttt acttttgttt aacctaaaga taatttactt ttaatctata tgaattatca
    46861 tataaataat cagaaagtta tctttataaa ttatagtttt agatttataa tttataaaat
    46921 atttatgttc tttgtaaaat tttttagaag cttctaatgt tatgctataa aactatcttg
    46981 agaagggctg attgaatgtt taataaaata tatgaaaaga aagaatttat agtttttcaa
    47041 gttaaaaaag gttatgtagt ttataatact agaaagagtt ttgaagaagg tcatacacat
    47101 ttaaagcatt ttgaagcagc aaagacagct attgatttag caataaataa aaagattcct
    47161 agaagcaaag atggatatta tctgactagt ttaattcgca taagtgatga tggttattac
    47221 attgataagt taagcgaatt gttatatgtt agagaacaaa aaggtaaaaa agaaaagtac
    47281 tgtaattctg gttatcagat gtaacaagac tagttgtgaa ttataagtac caatcataag
    47341 tacctttagt cttaaaatca aagagtatca gtaaatctat aaaaaaacaa cttactgata
    47401 ctctttttaa ttatatgatt aatatttgaa tttgatacaa aattttatac aaaagtttat
    47461 gttatctatt ctgattcttt gtctgcaaat tggctattat aaagcttctc ataaaatcca
    47521 cccttttcca ttaattcatc atgattcccc tgctctataa tacttccatt attcataact
    47581 aaaatcaagt cagcattgcg aattgtagaa agtctatgtg ctataacaaa actagttcta
    47641 ccattcataa gatttctcat tgctttttga agcataagtt ccagcctagt atcaacagaa
    47701 ctcgtagctt catcaagtat caatattgaa gggtctgata taaaagctct agctatggtt
    47761 aaaagctgtt tttcccctag agatatatta gatgcttctt catttaaaaa catattgtat
    47821 ccatctggaa gagtggttat aaagtgatga acatttgcta cttttgctgc ttgtataatt
    47881 tcttctttag tagccccaaa tctaccatat tctatatttt caaaaatggt tccattaaat
    47941 aaccatgtat cttgaagcac cataccaaac attgaacgca aatctttacg tttcatatct
    48001 cttatatcta ctccatcaat tttaatagca cctttcttta catcataaaa cctcatcaat
    48061 aagtttatta atgttgtttt acctgctcca gtaggtccaa caatagctac catctgacca
    48121 ctttttactt ctgcatttaa atcttctatt aaagttgaat tttctccata tccaaaatta
    48181 acatgctcaa atgtaacatt tccttttaca ttttctatct taactggatt ttcaacatca
    48241 ggtatttcct cttcttcgtc taaaatttca aatacacgtt ctatagctgc aaaagaagat
    48301 tgtattactg tagataactg agtcatttgt gaaagtggct gatttatctg ccatatataa
    48361 cgcacaaatg cttgtaaatt tcctactgtt attcctccac ttactgcata tatagaaccg
    48421 acaactgcca cagctgcaat tccaaagtaa gttactaatg aaaccatagg attcatcata
    48481 cttgatataa attgtgcctt aaatccattt tcacaaagtt cttgatttac ttttttaaat
    48541 tcttttatag aatcttcttc tttaccatat agttttattt cattaaatcc agtatacatt
    48601 tcttgtactt ttccatttaa ttttccaaga gcattttgtt gcaatgaaaa tctactttga
    48661 gattttttta ctacaaattt agatacaaaa taactaattg gaattataaa aagagctata
    48721 agagccatat atatattaat tgtaaacatc attgaaagtg ccaaaattat gactaagatt
    48781 gagtttacaa tttgcataaa actctgctgc aatgcatttg atattgtatc tacatcattt
    48841 gatatacgac ttaatacatc tccaaaacta ttactatcaa aataactaat aggcaatctt
    48901 cttattttat tttcgacttc atttcttaaa tctctcattg tgttttgaat agcattcgta
    48961 agtagaaaac ttgaagtata cgtaaaaatt gcattggcaa aatatataca tgctaaaatc
    49021 accaatattt ttgttacata atcaaaattt acggaagccc ctggtactcc ttttgcgata
    49081 ctaattacat ctttagtcaa ttgtgttatt atcaatcctt cagttttagg tgcaagtgca
    49141 ttcatagtag ctgctgaaat tatacatata atagctataa taaaggaaaa tttatatttc
    49201 tttacaaatg gaagaagtct accaattgtt ttcttaaaac ctttagactt tttcattatt
    49261 ctaattcctc cttttttaac tgagatgttg caatttcata gtatatttca cagtttttta
    49321 ataattcttt atgagttccc attcctacaa cttgtccttc attgagtact ataatcttat
    49381 cagcatcaat aatagaacta attctctggg caacaatcaa tacaattgca tcttttgttt
    49441 ccttcttcaa tttagcacga agtattgcat cagttttaaa gtcaagtgca gaaaaactat
    49501 catcaaaaat gtatatttca ggtcttctta taatagccct tgcaatagaa agtctttgtt
    49561 tttgacctcc tgatacattt gctccacctt cactaatcaa ttcatcaaac tttctaggtt
    49621 tttttgatat aaactcatat gcttgtgcaa cttttgcgga atattctaat tctgactctc
    49681 ctgccttatg tttcccaaat ctaatattat ttgctatgct accagtaaaa agcagtgatt
    49741 tttgaggtat gaatcctatc ttttttctta aagcttttaa atcatattct cttatatcta
    49801 ctccatttat cttaatgcta ccttctgtta catcataaaa tctaggtatc aagttaatta
    49861 gagtactttt cccactacca gtacttccta taaaagcgac tgtttcacct gtttttgcag
    49921 taaatgaaat atccttaagt acagaagctt ctccatctgg ataggtaaaa gttacattat
    49981 caaattctat gataccctta ttttcagtat ccttaattcc attttcagga ttttttatca
    50041 atggttcttc atttaatagt tcttttattc tatttgcaga tacctctgct cttggataca
    50101 taataaatac cattgaaaaa agcataattg aaaacatggc atggaaaaga tattccaaaa
    50161 atgcaaccaa ctgtccaact tgaagctctc caatatttat cttctcactt gaaatccaaa
    50221 atacagccaa aacagctata tttaatagta aaaagaatgc tggttgagta attgacataa
    50281 gcttaaatag tttcttagaa acattagcat aatatgtatt tgttttttca aatcgctctg
    50341 tttcataatc atcatttcca aaagctctaa ttactcttat tccagttaaa ttttctctcg
    50401 atataaggtt taatttatct agtccttttt gttgttttga agatattgcc tgagatactt
    50461 ttgctattat tgctactcca ataattataa aaggtactga tatagccaat actatagaaa
    50521 gttccacact tgttctaatt gtcattatta tacttataat gaacataact ggagttaata
    50581 aggcagtcct tagtagtata ttaacaaatt gttgaatttg aaatgcatca ttcgttgttc
    50641 ttgtaatcat tgaagaaatt ccaaatttat tgtattcagt atgtgaaaat tcttgtgatt
    50701 tctcaaatat gtcatttctt atatctctgg ttacacttgt tgatacctta gcagaacaat
    50761 aacccaacaa tatactacct acaaccccta taatagaaat aataacaatc actattccca
    50821 tttgttttat gtaagctata ttctgatttg caataccatt gtcaattatt ttagctatta
    50881 tagttggaat ccccaactcg actaatgcaa aactaaatat agccagtatg tttaatacta
    50941 ttaataactt ataattcttt aaataactta aaattaattt catatttttc tcctttctta
    51001 atttttgaga tagtgtaatc atgtaataat actaaattta taaatgctta attttataaa
    51061 aaaaggagaa aagagttaac gcactctctt ctccagcata ttctttttaa tacaccctcc
    51121 gacaagtcgc ttatatgtta acatatttga caagtaatgt caattcatat agccaaaagt
    51181 cttttaaatg cttcgtttca taacattgta acaaattggc atgtaattta tttttgtttt
    51241 ttactgtatt ccaattttac aagttctcca atattactag ctttcttaaa attcaaatta
    51301 gtcttatcta taccagactt aaatagtgaa atccctctcc ccaatattat tgtcaatata
    51361 tatatataat aatcatcaat taagttatct ttcataaata attttactat atcgcctcca
    51421 ccaactatcc aaatatcttt tttatccttc tcttttccaa tttgctcaat taatttttta
    51481 ggttctaaat taatttatag tgtgattatt ctatgtaaac atcacttttt tactcattca
    51541 ataataccct attaataaat cctcaaacta atttaaataa actttgtata aaattaaaaa
    51601 tatattatat aaataattta aaactacact aacttattga aaattctaat tattacaaat
    51661 aaatattgct ctctgttaac gctagttcta taatataatt ataagctatt aatattataa
    51721 agccgatatt actaatataa taattataat acttatagtt aatttacttt tgttgaaagg
    51781 tggtaaataa catgaaaatt tcagaagtcg ctactccaaa ttttttgcta gatttagacc
    51841 agttagaaaa gaatatagat aaaattcaac aaatttgtac aaataataat aaacaacttt
    51901 ggcctatgct taagacacat aaaagtactt acatagcgag acttcaaaaa aatgcaggtg
    51961 ctagtggttt tctagttgga acacttgatg aaggtgaagc tctgataaaa gcaggttttg
    52021 aagatattat gttggcttat ccttacatgg gagaagtaaa tataaataga attgtgacta
    52081 tggcatcaaa aataaattta atctgtgcta cagataatat agaatgtgct aaggcttata
    52141 gtgaagcttt ttctaaatca aaaatagagt gcaagctact tattattgta gattgtggct
    52201 tacaccgttt tggggttgaa ccagaaaaag tggttgaact tgctactgaa atttctaagc
    52261 tagaaaacat caagattgtt ggtataagtt cgcatccagg tcaagtgtat ggttgtagtt
    52321 ctccagaagg tgttccagat gtttgtgttc aagaagattc ttctatggag aaagcataca
    52381 aagctctaac tgataatgga tttaatatag aaattgtcgc ttctggtagt actccaacag
    52441 ttgaatctga agctgcttct aaaactataa ctgctgtaag acctggaaac tatgtatact
    52501 atgatgctat acagataggt ttaggttgtg ctactgaaga tatgtgtgct cttactgttg
    52561 tgtgcactgt aatctcaaaa aatagcagtg gatactattt aattgattgt ggaagtaagt
    52621 gccttggact tgataaaggt gcacatggaa atagtagtat tgttggttat ggtagggtat
    52681 gtggacatcc agaacttact attgattcac tttcagaaga agttggaaaa attaaagtaa
    52741 atggtgaaac tgatataaaa attggtgata gaataagaat tatacctaat cattcttgtt
    52801 ccactgcaaa cttgacaagt aatttattag gttttagagg agatacaata gaaaaagtca
    52861 tagaagtaga tattcgtgaa aattcaaaac aaatcatcct ttaaaaacac atttattctg
    52921 atattaacat gaaagtatat ttttcttaaa attattgcta ataactaatg ttaatgttgg
    52981 ataaataaaa aacataaatt ctagaaatat aaatttaaaa gaagaggctg tctcaaaata
    53041 gagataagcc tctctttcat tgttgaaatt atgaaaatat acttgatttc atacaaaaag
    53101 agtatctcat gaactaaaaa gttcactttg agatactctc tttttttaat taatatacac
    53161 attaattact atcttagttt attactatat atcaattact actcattaat ccttgtatat
    53221 attaattctt atatatcatt tatagttagg ttataatttt atgttattta cattaatccc
    53281 ataacagtga gtaaaactct tactatcaac atccccacta ttgcatcaaa tataggaact
    53341 gccaacagta acattctata cttagtactg gcatttgcta caagcactat tctggcataa
    53401 tgaccaacta gagtacccat taacatacat ggcataatta atatggttgc ttgtggtgca
    53461 gtgattagac cttgtgcaaa taagtttgca gctgtagctg ctcctgctgc cttagcaaag
    53521 aatgctgcta tcaaaactac aacagattcc ccaggtagtc caaaaatacc cattactggt
    53581 ccaaaaatat ttcctaaaat atcaactaaa ccagttaatt gtaaaaactg cactattaca
    53641 taacccatta tcatggctgg taatatctgt tcaacaccta tgtaaaaacc tttttttgct
    53701 ccccccataa atatctcaac agcagttttc ttaggtttac tatttacaat tacatttcca
    53761 gtattttgtt gactcatact acacagcctc cttagcctta ttctttttat ttcttacacc
    53821 tatgattaat ctaactagat tagcaccaag tattttacag aatatctcta taagtattat
    53881 tggacctaat gccaataatg ctattggtaa aagtggagct tgagtattta ttgtgttcag
    53941 tataacagct gaaccagcat attgatatgc tacaaatata gttctttcat catctgttat
    54001 ttccccactt tcaaataatt ctttcgtcat tattgaggct acatctgaac tagtaaatga
    54061 agatacaaat gctattcctg ctattccagg tataccaagc agaggtctaa gcaatggatt
    54121 aaatatcttt gatgccgctt tcattcctcc catagattca actacatcta taagacctac
    54181 agcaaaacag acagttggaa tcaaagtcag agcaaataag aaaccatctt ttgctcccgt
    54241 tcctccttta cccataaagt ctacaccatc tgcaatagta ccaaatgaac ctgtcagatt
    54301 agcaaaatct aatgctttaa ggaagccttg actatcttta aacactcctg aaaataatac
    54361 tatcaataca aataaagata aatacccttt ccatgttacc ttattatctc cagctgttac
    54421 atccatataa aatccccctt tatacctcaa atttatctaa gtttaaaata actgtagaaa
    54481 ttaatttcac cctctcaaac atagaatcta ctactgcata ttctcttgat gtgtggttcc
    54541 attctcctct tacaccaaat gaacagattg taggaacgtt tgcaatagtt aaataacttg
    54601 catctgaact acctccaagt ctcttagcat ttaattctcc aaatccattt tcttttgaca
    54661 cttcatttac aaatgtatga aatctcataa catcttctgt agtttcaaat ggcatcattt
    54721 cacttataaa attaacttct gtagaagttc cttctatgta agtttcttta catatctctc
    54781 taacttgttc tttaacattc tccatttctt ctactttttc aaatctaagg tctactttta
    54841 tactacaatc ttctggaata gaattttcca ctcttccacc cttaatcaca cttacactta
    54901 ctgtagttcc aacatctaaa ttagtcaatt tttgtatgcg tagtatctta ttagccattt
    54961 cttctatagc atttcttcca ccttcaaaat cattacctgc gtgtgtttct acacctttta
    55021 catgtatatt acatcctatt cttccttttc tacctacaca caaactatta tctactaatc
    55081 cagtttccat attaaaagca caaagaccac cttttgcttc cctcaatata acatctgcac
    55141 ctgttgaatc cttatgtcct atctcttcat ctccagaaaa tactattttt attggtcttt
    55201 ctttgtatcc tattttattt aatgctttaa tagcatataa ggaaattatt ataccaccct
    55261 tcatatctaa aacacctggg ccatatgctt ttccttctat tattttaaat ggattctctc
    55321 caaatgtacc tgtttcaaag acagtatcca tgtgacctga aaaaattatt ggctttttat
    55381 ctatattgga accaagtgta cctatcaatg tactaccatt atctccaaca tctactaact
    55441 cgcaatctaa accttctttt tcaaactcaa actttaatct cttagccaat ttatttacgc
    55501 tttctttaca atgtgtatag ctttccatat taacaatctc tttccataat gaaactattt
    55561 cttctctatt ctcatcaaca aacttagaaa tttcatttaa atatgtattc ataatcttct
    55621 cctttatttt caaataaaat gtatttcttt cctaaaatct aagtttattt taggaaatct
    55681 taaaagacta gtccttaatt acaaaatagc acaattattc ttgacagtca acatattttt
    55741 cagtcttttt tatattttct gatttttctt tcaattttcc ttttgtaaaa taaattgacg
    55801 cgttttatct actattataa aatgaggtta taaattgatt gattagttac aatactctcg
    55861 aaattttaaa ggaggtttta tgaaatataa aattggtttt ttgacaacat cttattttat
    55921 gtcagataag tttaaacaag ctcttaaaga attaaatgat acttgtgaaa ttacatttat
    55981 tgagcttaaa ggggagaatc cttttaataa tttacctaat atatactatg agcaagtaga
    56041 taattttgat ggatttgtat gcagtggtat agttccatat agtgaactta taataaatat
    56101 aaaagacata aaagttccat taaatttttt aaagcttgat gaaaaagatt tttataaata
    56161 cttattcaaa ttattaaata gccaaaaaga tattgacttt acaaaatcat ttatggattt
    56221 tttaagagag gacaacaatt attatgatat atattcatta atagactcta ataaatgtcc
    56281 atatacaatt aaagatttta atatccctca acctgtatat gactttaaaa aattaaatga
    56341 taaattattg gaaatacatt tagacttatt gcgagataat aagattaacc tttcttttac
    56401 tagaaattac atcattagtt gtgaacttaa taaacttgga tatgattcta tttacatggt
    56461 tccatctaca gaatcaatcc tgaatacatt taaatctctg gtgaatgaaa taacattaca
    56521 aaacttagac aaaaataaat ctgcaacttg tatagttaca gtaaatagtt cagagtacat
    56581 aaatgaagat ttgatattta aaaatgatga gatacaaaat caaatatata acactatatt
    56641 aaattctcta tcaagacatg gattctatgg agttcaggtc aaaaaaaatg atatgaaaat
    56701 agagatacac actactaaag aaatgttaaa caatatgacc aataactata cagatttttt
    56761 tatttcagaa gatttaaaag aaattaaata tagcttaaat ataggttggg gcattggaaa
    56821 tacaaatatt catgcagagc aaaatgctcg tcaagctaat ggtaaatctg cttctttgaa
    56881 tggaaactgt acttttatag taaatgatac taatgataca ataggccctt tatattcaaa
    56941 taaaaattct aataatattg aagatagctc aatagccaat aaagttgctt cttttatacc
    57001 tctatctaat acaaatgtat ctaaaatcat gtgtatgatt aatgatagaa attcaaataa
    57061 tgtgtcagca gaaatattag cagactatat gaacataact cttagaagtg ctaatagaat
    57121 attgagtatc ttatataaag caggaattgc aactattgta aatacaaaac tcgataatca
    57181 aagaggaagg ccaaaaaaaa tatataaagt tgatttctta tcttttctaa ataagatgca
    57241 gaaattaaat tcttaatatt taatttttta ataaaagcaa tgtattttct ctaattttaa
    57301 agaatcaaaa tgtttaaatt ttattagaat atacaacttt ttactttaaa atacctaaac
    57361 cttttatcag tgaaataaac cataatatat cgtaacttat tagaaaaaca aaataatttc
    57421 atatacaaat aataaaatag ccactacaat ccataatttg cagtggctac tttttacact
    57481 atcacttatt acttattttt aaaaattaat tgtatttttt aaataattta tacacaccta
    57541 ttatcagtaa agcaataaat acaagtatat ctataaaaat aattattgta acatgtctga
    57601 caatattgta aataaaaaat atagagaata tttctataga caacgctaca aaacacataa
    57661 aattcgttaa ataatctgca atagtatcac tataaaaagc atgtatttgt aaccatctcc
    57721 atataaatgg acgtaaaagt attctgaata caacacaaat gacagggtag gcataaataa
    57781 attttttatc tacataatta gctacaccat tattccattg gataggaatt tgaggtggca
    57841 acttttgaaa tactgttatt gcaatttaaa cactacctct ctattctatt aatttataac
    57901 tataatatag tcttaaatat tttcactcta taaaattgta tatttagtat atcttaaaat
    57961 caaactaatt atcttttata agttttcata caataatatc tcctttcaat aatatatctt
    58021 tttttatatt taaaactatc ttattttcta taaaataatg tataaacaag gtttacagtc
    58081 aagtattttt attatttttt tgatttcata aaaaaagcat agcaaattat ttgctatact
    58141 tttttactaa attatttatt ctatatctta tatgaactta ttgtttaact actacacaat
    58201 actagcttta atcatcatat tttttgcaaa catactggct tcatatattt cttgttcatc
    58261 tggatgactt tgagattcct catggttatt taattgttgg cgaatatgtt cgttgtcagg
    58321 agaaagtttt aacatctctt tatattttgc tttcaatcct tctgctatct taccttgaca
    58381 tatgaatgaa cctagtattt cattatcttt tgggataatt gattctatct tttctattat
    58441 actagctcca taacttcctt ttttacttgc tcccattgtt ccaaatagtg ctatcttttt
    58501 attgtgaata ttttcaagca cttgttttga atttttatca caaattccct tgtccaccca
    58561 atagcctaag aatactatat cataattttc atagtcaata ccataatcaa ctaattcatt
    58621 tacatcttta atgtcttttt cttcttgaat ttcatcatat atagtataag caatcttttt
    58681 agtatttcca gttaaacttg aatataaaac taaacttttc atgatgtctc ctcctcttta
    58741 tctaaatatt tatctaaatc accaaaaata tattaacctt aacaaattat ttatttttat
    58801 aaggatattc taatctagta tacatccaaa ctgagagcgt gtttgtaaag catctgcaca
    58861 cttcttatta caataacagc actttttaat ctcatctact cttccatctt ttactttctg
    58921 taaccaatta ctatctgcta gtaattgtct tgacatagaa accatatcta ctctattatt
    58981 tgaaataata ctttctataa aatctgggct agacaatttt ccaaccccac atactggaag
    59041 atttgtgtgt ttctttactt catcagctaa atataaaaaa cagccttctt ctttaaagta
    59101 aggatgatta ttggttggta tagtatcttc taattttgaa tgatttgcaa gtgttacatg
    59161 aaaactgttt acacctaggg attcaaattt tttaacaaaa tactcaactt ccgaaagtaa
    59221 aactcctgca tttccataat gaggattttc ttgtcttatt gcaaatttat agtctattgg
    59281 aatgtcctta acttcatctc ggatactact aattatttcg cttgcaaatc tacttctatt
    59341 atcacacgtt ctaccatatt catcatttct attattaaat atacttgatg aaaaacttcc
    59401 tacaagccta tcaccatgaa tttgaatcat gtcaaaacct gctttttttg cattcaatgc
    59461 tgttacttta aataaattta taatattttt aatttcttct ttgggcatat ttgttatgta
    59521 atcttttact ccgtcattca ttattttttt tatctcatca tgtgaagtaa ttcccatatt
    59581 catagtatct ttaattaaat taacatcata atctgaacaa aatagttgtg ctgatacctt
    59641 tgcaccagaa ttgtgtattt catctacaat tttcttgtat ttcatgactc catcttcatc
    59701 cgataggctt atagctacct tatgaaaact aggtctaata gatacatctc ctagagtaat
    59761 cagagctact cctgacttag caatatcaga aaacttttta attttttctt ccaatttaag
    59821 acccatgctt gtaggtgcaa aagatactct attttttaat tccaaatttc caattttaat
    59881 tggttttaat aaattacaat acatcctatt cttcctccac agctgttttt atatccttta
    59941 ccacatgtaa attctttacc agttctttat attctgtatc cgagaactct ttttttatgt
    60001 gattatccca ctttgaaaat atgtttttta tatcccctaa tacttcctca cccttatctg
    60061 ttactctcaa ctgaaaagct ctactatcat tagggttttg gattttttct atgtatccat
    60121 cttgttctaa cctagatata gttcttgttg tatgagcttt atcaacttca aattctgtag
    60181 aaacatcatt caaagaacaa ttcctatttt tattaatata aattaaatag aaatatagac
    60241 cattcgtaag tttatatggt tttaaacatt tttcacaata aagtttaaag tttttttgca
    60301 agttacaaat aatatatgac aaactttcat actgtaaatt tttaaaatcc atcttaaatt
    60361 tcccttc
//
LOCUS       DAYAPA010000006        60264 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.006, whole genome shotgun sequence.
ACCESSION   DAYAPA010000006 DAYAPA010000000
VERSION     DAYAPA010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 60264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60264
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.006"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(2..1102)
                     /locus_tag="ACGI59_000302"
     CDS             complement(2..1102)
                     /locus_tag="ACGI59_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012815981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HFG9738115.1"
                     /translation="MNLLLVKFNNLLKQFQTHKEEYEQKIKDVMESGYYILGNEVKHF
                     EEEFANYVGSKYCVGLASGLDALIIAFRALGISKGDEVIVQSNTYIASVMGISINGAT
                     PVFVEPNEYYNIDSSKIEEKINSKTKAILVVHLYGQASNMKDIQELCDKYNLYLIEDC
                     AQSHGAKYDGKMTGTFGDIGCFSFYPSKNLGAFGDAGAIVTSNEEIADKVKILRNYGS
                     EKRYYNKFVGYNSRLDEIQAGLLRVKLKYLNEIEIEKSKIVDRYLNEINNPKVILPKI
                     ENNCTHVWHLFVLQVEKRDTFMEYLKKSGIESIIHYPIPPHLSEAYAYLGYKKGSFNI
                     AENYSDKVVSIPLFIGMEDEEVSYIIKIINEY"
     gene            complement(1180..1587)
                     /locus_tag="ACGI59_000303"
     CDS             complement(1180..1587)
                     /locus_tag="ACGI59_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FdtA/QdtA family cupin domain-containing
                     protein"
                     /protein_id="HFG9738116.1"
                     /translation="MDKYNLIRFVSIGNEYIGNLVALESNKVIPFDIKRVYYIYDVPN
                     NVKRGFHAHKNLEQVLVCLNGSVKIKCYDGVKETIFNLHKNDVGLYIGTGVWHEMFDF
                     DEKTVLISIASEHYNEEDYIRDYKEFLKYLNNV"
     gene            complement(1681..2625)
                     /locus_tag="ACGI59_000304"
     CDS             complement(1681..2625)
                     /locus_tag="ACGI59_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase"
                     /protein_id="HFG9738117.1"
                     /translation="MKLITHEQIMSLNISQKQGYEWIKESFLTKKDCQLPPKISMVEE
                     GHVFYNVMPSLNHNHMIGGIKMVTRLPGRNPSLNSEIFLYNMEDAKLKAILDGNYITA
                     FRTAAVAALAIDTFAKKDFNTIAIMGLGVTASAIMNMLSYTLKDKNINIKLLKYKDQA
                     ELFVKKYEKNEQFSFEICDTHEELIVDSDVVISTVTYTDEYLSEFKWFKKGALLLPVH
                     VMGFGNVDYLFDKVYLDDYNHLKHLENIDRYKYSCEFYDVLSKQAKGRENDDERIISY
                     NIGLSIHDILFANKIYELCETLSCEEIELKEPKEKYWI"
     gene            complement(2645..3877)
                     /locus_tag="ACGI59_000305"
     CDS             complement(2645..3877)
                     /locus_tag="ACGI59_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HFG9738118.1"
                     /translation="MKRLMILGSIKYFENIVISAKKEGIYTIVCDNRIDTPAKKICDE
                     AIDVDVFDFDKIKSIAIDKKIDGILTGFTDSLMKPYVYIANELNLPCVISCNQLESVT
                     NKAIMKKYFKNNAIPTTDYCIIESLKDLEKIQGLNFPLVIKPVDGYGSRGVYFVSNIE
                     ELIEKFEYSKEASQDGQVIIEEFYESEEIQGLAWVHNGESHVFYIGDRELVNIHTGRA
                     GKPDRLLYPSKYCFQYEEEIKSIYQNITQAFDIKNGPLYVQMLVGAYGIKVSEVMPRL
                     PGGCDYMAISQITDLDIGSLFANFSVNNEVNFDEIKKYNMHLSKCVYALPIYIRPGTI
                     KKINNIDKIENLDYVTQFLLLVKEGDTIELYEDERQDCGRVYGIANNIFDANIKKKCI
                     HEMIEILDENNENMIENF"
     gene            complement(3969..4964)
                     /locus_tag="ACGI59_000306"
     CDS             complement(3969..4964)
                     /locus_tag="ACGI59_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HFG9738119.1"
                     /translation="MNNIHINKFEKSCERNWDYFVENKSINGTFLQTRNFLNYHEEGK
                     FTDHSLIICKGEEILAVVPACETYEDGERIFFSHLGSTFGGIIIRSGFNDIRHVNAIT
                     NKLEIYLREQNFDKVYLKNTSRIFCKEDTSLLEYFLFKNNYMFYDELSMFLELSKYKE
                     DILANFKYSKRRDFKYSLKNNLTFKRLYSDEDIVCFFNLLSNCLDSKGAKVIHSVEEL
                     LEFKNNRLKDIVEFYAVYIDDIMICGSMVFNFQERVYHTQYLASNSTYNKYYPMIFLY
                     YNLIKDALERNFKYLSFGISTLNRGKYLDEGLATFKEGFGSSAICNRTYYKNINK"
     gene            complement(4973..7087)
                     /locus_tag="ACGI59_000307"
     CDS             complement(4973..7087)
                     /locus_tag="ACGI59_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFG9738120.1"
                     /translation="MNNDKICFITCVNDERLYEECLVYINSLDIPKNFSIETVALRGA
                     DSIASAYNKAMRETDSKYKVYLHQDTFIINKNFIHDILEIFKKNTDVGMIGVAGVKDM
                     PVNGIWWDSDKKAGKVYDSHTGKMDIYEFSNFEECFNEVMAIDGLIMITQYDVFWRED
                     KFDGWHFYDLSQSMEFRQNKYKVIVPKQNKPWCMHDSGVVNINNGYDEYRIKFVKEYI
                     NLDLYRENFYEFDKKKASTILKSNKHVMDCMKEELTEQLRLKNYKDVLGIVSKIAHFS
                     VFNHTGLYSCEECERALLECANNLPEIENTKLFTKKDLKRRKVLHVLSEGYSTGGHTR
                     LVKNWIEIDKDSVHSVVTTWQSDTLPDWLLKAINDSGGEVISLDLISDDYLKRAVALR
                     KIAYDWADIVILYVHMWDTIPVVAFGVDGKIPILHVNHYDSTFWLGTSIADIVIDFSE
                     ASQLITLDKRGIDKSEILPMPLKEKEVFSKSEVRKRYNLEDDVTIILTIASERKYNSV
                     NDINYFKIAKTLIENTDNTIIIVVGVDTENLYWKTLSNVTDNRVICFGTTANIDDFYK
                     ISDIYMESFMVGSHTSKLDAILYDLIPVKFKNEVSPIFTNMWNFIDKFDYKNIKEVID
                     MIKRYNTSEFDRDRIKEKQSEIKHFVIDKHCIEIKNYIDKIYNEANHHKVKYDLYKND
                     EIEDYQLFVALYGYMVKNKNMS"
     gene            complement(7080..8957)
                     /locus_tag="ACGI59_000308"
     CDS             complement(7080..8957)
                     /locus_tag="ACGI59_000308"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFG9738121.1"
                     /translation="MNIPIVVNEKSKDIIKDWQYTCTNEDIKFTSIIILTYNQIEYTK
                     LCIESIRKFTPKNKYEIIIIDNNSSDGTVEWLKGQSDIKSVYNNKNLGFPKGCNQGIE
                     LATGENILLLNNDVIVTPNWLYNLNKALWSQDNIGAVGPITNACSYYQQIDVDYKSIE
                     DMFIFAHKINKSNINLWKYRLKLVGFCMLIKKTVLDNVGVLDELFTPGNFEDDDLSFR
                     MIESGYKLLLCQDTFIHHFGSVSFKENRNKYTDIMYINRRKFETKWGFNPEYSGMIRT
                     DLISKIDDSLEANINVLEIGCGIGATLLEIKNIYKNASIYGIEISESAGKLAKYSADV
                     LIANIEKVKLDYKKDFFDYIILGDVLEHLIDPWKIVNELKQYLKKDGYLVSSIPNIMH
                     VSVLRNLVLGNFTYEDSGILDKTHLRFFTLNEITKMFNDSNYIINSITATSMPLSEID
                     NSFIDTICKESCKEMRQQYESYQYIVKASKILDTKRYSSISMINLKYALMRIDNELDV
                     DSNLSYIFDNYNITEPCFVDDIEYLIDTFVINKATLLNRLGVEAFNRGLGDFSINMFM
                     TVLEMDRDDIDSIYNITSVLCELEEFDTAYNLIKNSSEKVQSESDIQEILCFIEGKKY
                     E"
     gene            complement(8981..11122)
                     /locus_tag="ACGI59_000309"
     CDS             complement(8981..11122)
                     /locus_tag="ACGI59_000309"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFG9738122.1"
                     /translation="MLLSIVMIVKNEEKILEKTLKSLTTLRNSIESELIIVDTGSTDD
                     TIKISKKYTEKVYFHNWNNDFSSMRNISISYAKGEWLLILDADEILIDSSTIVKFFKD
                     GLDKKFNSASIQLKNLYSYDKKLYGYCSVLRLFKNVDFRYHGKVHEQPIYKNPIFNNV
                     ADFEHYGYLFEDEEIRLNKVKRNEKLLFQELKENDNSPYTNYQLGKNFIILGKYQEAL
                     YYLEKSYELYSKLELAPGYLITSLVKTYLYLGKHKKCERLCLKYIKKDMNNIDIYYYI
                     AQAQVDLGKYENSIDSYKRYIYLLDNYEISTQANSLFSDTDTVGLRDEAIITLIKIYY
                     KLEKYEFVISEYNNIEDVEKKKNVYFSLFMSLYKLNRFEDIKNYYKEVSNSKIEKNSF
                     YRNIEEVINNIKEDEKEYVYKILCDIEGNYGILNQIRINKCVSIDKCKEILNEEKEII
                     YAYIIKIAFEEKIDLLDIFYEMEYVWIEKYLKYLLALERSFNLVLYKYIISRPNTSDM
                     EKIRVYKAISKVVLESISLSDEKYKEIFYLYIMYSYKYIRYIYSNLSDFELIKFVCND
                     LDKFTIEFKHLLDIKNKVNNANEKLEYIHSMRKLLNEYPFYNKIIKILIGELEENIKE
                     SEEFKDLKDSFIISIENMIETGKINDAKLLVDDYSKTFVDDIKILNIKGILYMFENKL
                     DKADFMLKKALSLDVENEDTIYNIEYLKSLR"
     gene            complement(11215..12087)
                     /locus_tag="ACGI59_000310"
     CDS             complement(11215..12087)
                     /locus_tag="ACGI59_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HFG9738123.1"
                     /translation="MRVNTNVSALIANNQMGRNVNAQSKSMEKLSSGVRIKRAADDAA
                     GLAISEKMRAQIKGLDQAGRNVQDGISVVQTAEGALEETGNILQRMRTLSVQSSNETN
                     TAEERQKIADELLQLKDEVERISSSIEFNGKKLLDGSSTEIRLQVGANFGTNVAGTSN
                     NNNEIKVALVNTSSIMSKAGITSSTIASLNADGTSGTNAAKQMVSSLDVALKELNTSR
                     AKLGAQQNRLESTQNNLNNTIENVTAAESRIRDTDVASEMVNLSKMNILVQASQSMLA
                     QANQQPQGVLQLLG"
     gene            complement(12189..12524)
                     /locus_tag="ACGI59_000311"
     CDS             complement(12189..12524)
                     /locus_tag="ACGI59_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliT"
                     /protein_id="HFG9738124.1"
                     /translation="MDKLKEKLNLYKDISLQIINLIEKEEYIKISIKLGERQEIINSV
                     SEIDRNDFIQLYNRMELIEIDSRIRDILQGQLLEVKKELHEYKLTKQVNTMYYNLNRE
                     KVNIFNKKV"
     gene            complement(12527..14050)
                     /gene="fliD"
                     /locus_tag="ACGI59_000312"
     CDS             complement(12527..14050)
                     /gene="fliD"
                     /locus_tag="ACGI59_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HFG9738125.1"
                     /translation="MSSISPIRVTGLSGNFDMEGIIEASMIRDKEKVDKAKQEQQIVK
                     WKQEIYRNVIQESKDLYDKYLSINSPNSIVSEKAYSSTRITSSDESIIVAKGSAGAEK
                     INYQFAVSQMAEPAKFTIKLNSSEPIVRQFPPNASGASSLTIGDVNIPISEQDTTSTI
                     VSKINSLCADNDIKASYSEMTGELIISRKQTGSSSDINLRVIGNDNLAQQIANDNGIT
                     FINDAGGNKVANVYGKNLEADVTDEHGRVTHISKEQNSFNIDNIDYNVNSKGTAKLTS
                     VTDTEEAVKNMQAFVDDYNKLMDKVYGLVTTKKPKDYPPLTDAQKEDMTTEEIEKWEK
                     KAKEGILRNDDELRGFVEDIQSAFFGDGKNIIALRKLGINESENYNKKGQISFNADTF
                     SKALIDDSDKVYKTLAGYSSNYDDKGMFEKLKDIVYEYSGSSTSKLPKKAGIEKTASA
                     SENVYSKQIAEQERNISRLVEKMNDKEKRLYAKYSALESLLNQYSSQMNYFSQAQGN"
     gene            complement(14071..14427)
                     /gene="fliS"
                     /locus_tag="ACGI59_000313"
     CDS             complement(14071..14427)
                     /gene="fliS"
                     /locus_tag="ACGI59_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HFG9738126.1"
                     /translation="MELNLAELDKLSKEELLLMLVDGTVKYTNISKEALLNNDYLKAH
                     NELVRVQNIFTELMTTLDQNAGQWAKDMYKVYEFIKSELAIADENKDIKIIDDILPIV
                     KQIRDTWHEVYNQLKI"
     gene            complement(14427..14822)
                     /gene="fliS"
                     /locus_tag="ACGI59_000314"
     CDS             complement(14427..14822)
                     /gene="fliS"
                     /locus_tag="ACGI59_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HFG9738127.1"
                     /translation="MYGENPYNSYKQNAVFMASKEQLLLMLVDGAVKYTKIARAAILD
                     KNTQKAHRELVRVQDIFTELMVTLDQNAGQWAKDMYKVYDFVRYELSRANIRKDIQVI
                     DNVLPVIEQIKDTWHEADKKSKEERSRYK"
     gene            complement(14844..15056)
                     /gene="csrA"
                     /locus_tag="ACGI59_000315"
     CDS             complement(14844..15056)
                     /gene="csrA"
                     /locus_tag="ACGI59_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HFG9738128.1"
                     /translation="MLVISRKKDEAVLIGESIEVKIVGVDGNNVKLAISAPNNISILR
                     KEIYEKVKSENIKATNKNIKILKSLK"
     gene            complement(15050..15442)
                     /gene="fliW"
                     /locus_tag="ACGI59_000316"
     CDS             complement(15050..15442)
                     /gene="fliW"
                     /locus_tag="ACGI59_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HFG9738129.1"
                     /translation="MMKVTLKKGILGFENLKEYELLDIEDNDYLKEFNSTEEEGIGFV
                     VVSPFEIINEYEIVLNQETIEKLEVKSPKDVVLFNIITLGQTFEESTVNMKAPIVINI
                     KTNFGMQIILQDDKYSIRHPLLRGDNGC"
     gene            complement(15458..16390)
                     /gene="flgL"
                     /locus_tag="ACGI59_000317"
     CDS             complement(15458..16390)
                     /gene="flgL"
                     /locus_tag="ACGI59_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HFG9738130.1"
                     /translation="MRVSTGMMSSSYLNSLQDNLQRLDKVNRQVNTTKEINKLSDNPY
                     KAIKILNSKSEIKTMETYVENCKDTGNWLETTDTALDQLGKLLSDIKKTLVASGDGAY
                     SDDELKTLSNETNEKMKEIANALNATHEGKYIFSGSNTGVPPVECIDNADGSVSLKFN
                     SSLNLNKLNDSLSMDIAQGISVDYNVKLSSLGFDPTKTDPFEELNNISKKLANPDADA
                     IKELTTTCLGDMEQLIENTVNVRSIYGTKANTVEAMKEKNDDSLIQLKDILSQNEEID
                     YGEKLVQLKAAELTYQASLQTGGKLFNVSILDYI"
     gene            complement(16409..17719)
                     /gene="flgK"
                     /locus_tag="ACGI59_000318"
     CDS             complement(16409..17719)
                     /gene="flgK"
                     /locus_tag="ACGI59_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HFG9738131.1"
                     /translation="MAGLFSILNTANSGMNAHQKAIQTISHNISNLDTDGYSRQRTEF
                     ATNSPMYMPSLSNSIARGQLGTGVHVTDVTRARNSFYDYQFRAEAHKYGKTVSKYDYY
                     NTIETILNEPSDYGISAGIDDFFKGWESLSKDINSDSKKSLVVENASNLATLISESYK
                     KLDKLKEDIDSNIDTEIKQINDMLKSLEDLNKSIDIISGSGSTPNDLLDERDRILDNL
                     SFKLDFENSDVKNMLADGKLELNELKNPDGTWKTGISGTLQGLFEMHGKIDTYKSDLK
                     DVSDGLAKQINDVYNTSAGITVTDFFITSNVDGEDIIKINPAIKSNPNELKLTADEAS
                     KIAKLKDEKIDIGVAGGKVSTISDHYKAFAESIGLDSQKVNQDEANQRKIINNIDNSR
                     MSVSGVSLDEEMTELIKIQRSYQASAKVMSTAVQLLDVVINGII"
     gene            complement(17737..18153)
                     /locus_tag="ACGI59_000319"
     CDS             complement(17737..18153)
                     /locus_tag="ACGI59_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HFG9738132.1"
                     /translation="MITDLKVIIYEEKNIISKMMELLKEQFDYIVEKDLENLNMLNPK
                     LEDISRELASIEIKRRQLFGNDISMSEVVENSNDGYLKEIYTDLKRILALAKNQQESN
                     DSLIKKELIFTKKMINFIKPADKQTTTYNSYGNIRK"
     gene            complement(18158..18433)
                     /gene="flgM"
                     /locus_tag="ACGI59_000320"
     CDS             complement(18158..18433)
                     /gene="flgM"
                     /locus_tag="ACGI59_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HFG9738133.1"
                     /translation="MDIKNVSSNIVVNNYLKNTSGIKKVEEVKKINKTDKIELSKFAK
                     EMASVSNMSDENIRNKKVDEIKNALENGTYKIKPKEIAKKMIEDMRG"
     gene            complement(18584..18898)
                     /locus_tag="ACGI59_000321"
     CDS             complement(18584..18898)
                     /locus_tag="ACGI59_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HFG9738134.1"
                     /translation="MNSKKEDIYELNFGQLIEEKSGVIEENQNVFNAKVKVAVSIGNT
                     TETIKNILDLKEGSIIKLSKNVDEKLDIYANDRLFAYGESIITNEKLSVRLSKIEDSD
                     VN"
     gene            complement(18922..19350)
                     /locus_tag="ACGI59_000322"
     CDS             complement(18922..19350)
                     /locus_tag="ACGI59_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaaR family protein"
                     /protein_id="HFG9738135.1"
                     /translation="MGINIDKLNISPSINMEVKKLKESNKFSDKLNSLKQSSSLEDIK
                     RNLESVKKAGERLVLTQNYGDISIYKNAVKEYLKSVVDNMYSLDKNSSFWEHQYYKNV
                     KIVDSKLEDMTSKLISEEKENIDIVSTVDMIQGLLIDMYR"
     gene            complement(19359..20027)
                     /locus_tag="ACGI59_000323"
     CDS             complement(19359..20027)
                     /locus_tag="ACGI59_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFG9738136.1"
                     /translation="MNEISSLINILALTQLSGSNTTNQCNCTYNNSSGFDMIMMTLLK
                     ALSQNNQHTSNSYNLLSNSENLFNELDNVVNNTTSRFIDVDTKDKNVKLRIENAVNQA
                     SKKYNVDSNLIKAIIKVESDFNPNTVSSAGAKGLMQLMPENCRDLGVTDPFNIEQNID
                     AGTRHIKEYIDMFGGSIEMGLMAYNGGPGRMKSRGVESISDLYKMPKETQNYIPKVMK
                     YYRG"
     gene            complement(20057..21040)
                     /gene="rfbB"
                     /locus_tag="ACGI59_000324"
     CDS             complement(20057..21040)
                     /gene="rfbB"
                     /locus_tag="ACGI59_000324"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFG9738137.1"
                     /translation="MKKILITGGAGFIGSNFIHYMLNKYVDYFIVNLDLLTYAGNLET
                     LIEIQDNSNYKFVKGDISNREQVYKLFEEERFDIVVNFAAESHVDRSVENPDLFIKTN
                     IIGTQVLLDASIKYGVKRYHQISTDEVYGDLPIDRKDLFFTEQSSINPSSPYSASKAS
                     ADLLVSSYYRTYGLLTTISRCSNNYGPYHFPEKLIPLMILNALENKQLPVYGNGENVR
                     DWLHVYDHCTAIDLIIHKGNIGEIYNIGGHNERSNLEVVKMILNLLGKSEELISYVND
                     RPGHDLRYAIDATKIENELGWKAKYDFDLGIKETVKWYIENESWWKAVLSV"
     gene            complement(21078..21635)
                     /gene="rfbC"
                     /locus_tag="ACGI59_000325"
     CDS             complement(21078..21635)
                     /gene="rfbC"
                     /locus_tag="ACGI59_000325"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFG9738138.1"
                     /translation="MGKINVIKTKIEGLYIVEPNVFYDERGYFMESYNKKDFIEQGLD
                     MCFIQDNESSSRKGVLRGMHFQVKYPQGKLVRVTEGEVFDVAVDLRKDSQTFGKWEGV
                     ILSDKNKKQFYIPEGFAHGFLVLSEKAVFSYKCTNYYYPEFENGFIWNDKQISIEWPL
                     KGIEEIFLSEKDKKQKSFKDICTRR"
     gene            complement(21684..22553)
                     /gene="rfbA"
                     /locus_tag="ACGI59_000326"
     CDS             complement(21684..22553)
                     /gene="rfbA"
                     /locus_tag="ACGI59_000326"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFG9738139.1"
                     /translation="MKGIILAGGSGTRLYPITKCISKQTLPIYDKPMIYYPMSVLMLA
                     GIQDILIISTPRDIKAFEELFGDGSSLGLNITYAIQEKANGIAEAFIIGEEFIGNDRV
                     SLVLGDNIFYGYGFSERLENAVKRKGATIFGYHVSNPNSFGVVEFDKDFNVLSIEEKP
                     TVPKSNYAIPGLYFYDNDVVEIAKLVEPSDRGELEITSINSEYLKRGKLKVELLGRGM
                     AWLDTGTHKGLLDASNYVEVVQTRQGLYIACLEEIAYTKGYISREQLMKLAKPLIKTE
                     YGQYLLKLASEDM"
     gene            complement(22662..23534)
                     /gene="rfbD"
                     /locus_tag="ACGI59_000327"
     CDS             complement(22662..23534)
                     /gene="rfbD"
                     /locus_tag="ACGI59_000327"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFG9738140.1"
                     /translation="MKILITGSNGQLGKELVNQLEAINQSINQPKYVILATTRSDLDI
                     SNQTNVDNFILHNKPDVVVNCAAYTKVDACEDNIEIAYKINALGVRNLAIASEKVNAK
                     LIHISTDYVFNGFSKYPYREDNKTEPNSVYGKSKLMGEKFVEQFSHKYFILRTAWLYG
                     NGNNFVKTMIKLSLENKEVNVVNDQFGTPTSTVDLAKIIIKIMETEHYGVYHATCEGE
                     CSWYDFAKKIFKLKNIDIKVNPIKSNEFKCKAPRPLYSVLDNFMLKLIGLNSFRKWEE
                     SIEEYLERYDINEK"
     gene            complement(23740..27546)
                     /locus_tag="ACGI59_000328"
     CDS             complement(23740..27546)
                     /locus_tag="ACGI59_000328"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HFG9738141.1"
                     /translation="MLNTENKDSMVRRVLVEKREGFDLEAKALKKDLVESLHIDNIEN
                     LRILNRYDVEGISEEVYENAAKTIFSEPNLDVVYYEEIPKLNDERVFAIEFLPGQYDQ
                     RGDWAAQCVQIVNQGIRPAINTAKVYILSGKITDEEFSKIKDYCINPVDSREASLEKP
                     ETLKMETEIPTTVEVLDGFIDLDENGLKTFVSEKGLAMTLGDLQHVQKYFKDTEKRNP
                     TITEIKVLDTYWSDHCRHTTFMTEIENVKIEDGKFNDIVKEAYQMYLNSRDNVYVNRH
                     KDICLMDIATVAVKELKKNGKLNDLDESEEINACSINVDVEVDGKMEKYLVMFKNETH
                     NHPTEIEPFGGAATCLGGAIRDPLSGRSYVYQAMRVTGSADPRTTLEDTLPGKLMQRK
                     ITTEAAHGYSSYGNQIGLTTGQVAEVYDENFVAKRMEIGAVIAAAPKENVVRERPEAG
                     DVIVLLGGKTGRDGCGGATGSSKEHSEESILTCSAEVQKGDAPNERKIQRFFRNKEVA
                     QMIKRCNDFGAGGVCVAIGEIADSLDINLDLVPKKYDGLDGTELAISESQERMAVAIK
                     KENKDKFIQLAVEENLEATHVATVTDTGYLRMFWNGKAIVDINREFLDTNGVKQTTDV
                     HVTKVDEENTFFSSNEIVKDVKCASMKDKFTKVLSDLNVCSQKGLVEMFDNTIGGNTV
                     LMPFGGKYQATPTQGMVAKIPVLGGETNTSTIMTYGYNPKVGKWSPFHGALYAVVESV
                     CKLVAIGGNYSTTRLTFQEYFEKLGNNPEKWGKPFSALLGAFYAQSKFEIPAIGGKDS
                     MSGTFKDIEVPPTLVSFAVDTVDAKKVVSPEFKKADSKVVMLCVNKAENDVVDFEELK
                     RNLDKVRELIHGNKVLSTYALGFAGVGEAISKMAFGNKIGFKFSEEAEKAFTDDKLFE
                     ASYGNIVLELANDDLSMLEGYNYVVLGSTVKETSIFIKGEELALDELYKAHCSTLEPI
                     FPTKTEEVKSKIETISYISQGEAKKSSLSIATPRVFIPAFPGTNCEYDSARAFERAGA
                     NASIRVFKNLTYKDIEDSIDTIVNEIKSSQIIMLPGGFSAGDEPDGSGKFIATVFRNP
                     RVQEAINEFLTQKDGLMLGICNGFQVLIKLGLVPYGEIRVPSESAPTLTYNNIGRHQA
                     KIARTRISSNKSPWLAQTNVGDIHNIAISHGEGKFVASEDVMRELIANGQVATQYVDF
                     NNEATYDIEFNPNGSFYAVEGITSADGRVFGKMGHSERIGEEVYKNIIGEKDQKIFES
                     GVKYFR"
     gene            complement(27571..28821)
                     /gene="purD"
                     /locus_tag="ACGI59_000329"
     CDS             complement(27571..28821)
                     /gene="purD"
                     /locus_tag="ACGI59_000329"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFG9738142.1"
                     /translation="MKILVVGGGGREHAICWKLSKEKNVEKIYCAPGNAGIANVAECV
                     NIGDTNIEELLKFAKENEIGLTIVGPEVPLVMGIVDEFEKEGLRVFGPNKKCAQLEGS
                     KAFSKEFMIKHNIPTAKYKEYTNLEEAISEIDSFGYPVVIKADGLAAGKGVVIPENRE
                     DAIATLKEMMSDKKFGAAGDKIVIEEFLKGIETSILAFVDNDTIVPMASAKDHKKVNN
                     YEQGPNTGGMGTFSPSEIYTEELANKVKETVLEKTLEGFKKDGLNFKGILFVGLMITE
                     DGEKVLEYNVRFGDPETQSVLFRLETDLHEIMEAILDNKLKDIEINYSDDEAVCVMLT
                     SGGYPDSYEKGKIITGLENLDDDIVVFHSGTKMFDGNLVTNGGRVIGITAKSTTVKDA
                     AEKVYENIKKINFEGMHYRTDIGR"
     gene            complement(28832..30364)
                     /gene="purH"
                     /locus_tag="ACGI59_000330"
     CDS             complement(28832..30364)
                     /gene="purH"
                     /locus_tag="ACGI59_000330"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFG9738143.1"
                     /translation="MSKRALISVTDKTGVVEFAKELNKLDYEIISTGNTFKTLKENGV
                     NVMQVEDVTNFPEILDGRVKTLNPYIHGGILYKRDKESHVETVNEHKIHSIDLVAVNL
                     YDFEGTLKAGKSHDEIIENIDIGGPSMIRSAAKNYKDVIVVVDIKDYDSIIEKLKTDT
                     MTLEDRKKLSYKAFSTTGRYDALISSYFAGEVGDTYPDILNLTFQKEQTLRYGENPHQ
                     NGFLYSQSNAKNPILNYEQLGGKELSFNNLNDLHGCLEVMREFKDSEEVVSVAIKHAN
                     SCGVGLGKDAFEAYTKCYEADKVSIFGGIVGITSTIDKATAEKLNEIFLEIVVAYDFE
                     PEALEILKQKKNLRILKLAKIENSLQPYEMKYLDGKLLIQDRNNILAEKSENVTKEKP
                     TDAQLKDMEFGMRVVKNMKSNAIAIVKNGQTLALGCGQTSRIWALKNALENNKDKDFT
                     GAVLASDAFFPFDDCVTLAHEYGISAVVQPGGSIKDKDSIEACDKYDMVMVFTGIRHF
                     KH"
     gene            complement(30357..30950)
                     /gene="purN"
                     /locus_tag="ACGI59_000331"
     CDS             complement(30357..30950)
                     /gene="purN"
                     /locus_tag="ACGI59_000331"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFG9738144.1"
                     /translation="MLNIGVLISGGGTNLQAVIDGTESGEIKGQVKVVISSKQGAYGL
                     ERAKNHNIKAICETDEDKIIEILKENKIDLVVLAGYLKIISPKLVNEFRNKMINIHPS
                     LIPSFCGAGFYGEKVHQGVIDYGAKVTGATVHFVDEGADTGPIIMQDVVKVNQDDDAK
                     TLAKRVLEVEHRILKESISLFCENKLKLQGRRVFINE"
     gene            complement(30944..32020)
                     /gene="purM"
                     /locus_tag="ACGI59_000332"
     CDS             complement(30944..32020)
                     /gene="purM"
                     /locus_tag="ACGI59_000332"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFG9738145.1"
                     /translation="MLTYKESGVDIDEGNRAVDLIKGKIKGTYDGNVVGDLGNFSGLY
                     SLKDFVGMKEPVLLASTDGVGTKLKIAQMMDKHDTVGIDLVAMCVNDLICQGAKPLFF
                     LDYIALGKLVPEHIEKIVGGIADGCKMSGCALIGGETAEMPGMYGEDDYDLAGFSVGI
                     ADKEKIVSGNNVKSGDVLVGISSSGVHSNGFSFIRKIFLETYNYKMERYVEELGMTVG
                     EALLTPTKIYVKLALDVLAKHDIKAVAHITGGGLIENITRVIPKGLGLDINKKSWEKP
                     PIFKMIEGFNAVDERELHKSFNMGIGLVLIVDKENADDVVNFINNRENDNADYVDKKY
                     SELLEDKAYIIGEVVDSHEGVELC"
     gene            complement(32014..33381)
                     /gene="purF"
                     /locus_tag="ACGI59_000333"
     CDS             complement(32014..33381)
                     /gene="purF"
                     /locus_tag="ACGI59_000333"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFG9738146.1"
                     /translation="MCGVLGIYSNKDVTKELYYSLYSMQHRGQESCGLALLDDGEIKY
                     KKDMGLVGDVFKENELSKLKGNIGIGHVRYSTAGGSHVSNCQPLVGSCRKRQLAIAHN
                     GNLVNANYLKDMLEEDGYMFQTNSDTEVILYILARYYKGDIVESLKVTMDYIKGAYAL
                     VIMSQEELVAVRDPHGFRPLVLGKKGDEYIFASENCAIDILGGEVIRDVEPGEIIVVK
                     DGELKSYFYSENYKPVKKSCIFEHIYFARNDATIDNVNAYEFRIKCGERLAQNETVKA
                     DMVVPVPDSGWPGAIGYANASGLKISEGLVKNRYVGRTFIKPTQEEREIAVKIKLNPL
                     STIIKGKSIILVDDSIVRGTTSKQLVKSLREAGAKEIHLRITSPPVAYSCYYGIDTPN
                     RSKLIASSNNVEEMREYIGCDSLKFLDIEGMLDATEHKSTFCKACFDGEYPVKKIDKE
                     ELLSC"
     gene            complement(33393..34091)
                     /gene="purC"
                     /locus_tag="ACGI59_000334"
     CDS             complement(33393..34091)
                     /gene="purC"
                     /locus_tag="ACGI59_000334"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006772094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFG9738147.1"
                     /translation="MLLYEGKAKQVYSTDNENEYVVYYKDDATAFNGEKKAEISSKGI
                     LNNKISTIIFEMLKENNINTHFIKSLSDREMLVKKVEILPLEVIVRNIAAGSICKRVG
                     LEEGVVFDEPIFEISYKNDAYGDPMLNDDYAVAMKLATREELKFLREETLKINELLKA
                     FFLKLNLKLVDFKIEFGKDSEGNIILADEVSPDTCRLWDVNTNEKLDKDRFRKDLGDL
                     VEGYTEVLSRMNNK"
     gene            complement(34097..34573)
                     /gene="purE"
                     /locus_tag="ACGI59_000335"
     CDS             complement(34097..34573)
                     /gene="purE"
                     /locus_tag="ACGI59_000335"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFG9738148.1"
                     /translation="MKVAVVMGSKSDYPKLEEGIKLLEKYGIEVVARALSAHRTPEQL
                     SIFLKEIEDDTDVIIAAAGKAAHLPGVIASQTLIPVIGLPIKSSTMDGLDSLLSIVQM
                     PKGIPVATVTIDLGLNAALLALQIMTLKYPKLKEDLKSYREEMAQKVLEDDNNLRG"
     gene            35023..35535
                     /locus_tag="ACGI59_000336"
     CDS             35023..35535
                     /locus_tag="ACGI59_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFG9738149.1"
                     /translation="MLEVIKNRRSIRTYIDKNIEEDKITEILKSAMQAPSSKNAQPWE
                     FIIVDDKELLKQLSKSQHRAKHIEFAPLCIVVLGNRDKFLKPGKWIQDLGACTQNLLL
                     EVTNQGLAACWAGVFPKNKVVNKVRQTLDLPLKLVPYALISIGYSEEKNEFIDRFDEN
                     KIHRNVYKNR"
     gene            complement(35919..37082)
                     /locus_tag="ACGI59_000337"
     CDS             complement(35919..37082)
                     /locus_tag="ACGI59_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFG9738150.1"
                     /translation="MIKTHRVKLNLTRTQFELVREKQMESANCWNYIVNLSKEYYFEH
                     KQWIKKNDIQKLIKGKYNLHSQTIQAISDKFDANRKTISELRKKGNTKAKYPYKTKKF
                     YIIPFKASAINRNAKGNLKLSMSKGRYLELDFNVENIKTAEIVWRNGYYLYYTFDNEL
                     NGVVAKGTNTVGVDLGEIHSIASVTNKGVGLILSNREGRSIKQFRNKMYAYISKRLSK
                     CKKGSRQSKKLWRLKNKIRSKTDNQLMNLYHQTTRKFIDFCVEQKVCEIVLGDIKGVE
                     KDTKKKKRLNRVNRQKISQMEYGRIKDYIKYKAKEQGIEVKLVKENYTSQTCPKCSKK
                     HKPTGRTYSCTCGYETHRDIVGAWNILNKKHKYGLVDFRINHKQPINLKVSTV"
     gene            complement(37079..37477)
                     /gene="tnpA"
                     /locus_tag="ACGI59_000338"
     CDS             complement(37079..37477)
                     /gene="tnpA"
                     /locus_tag="ACGI59_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HFG9738151.1"
                     /translation="MYKSNKNIVYDCKYHIVFCPKYRKPVLVGEVEKMLKEILPYKAD
                     ELGAEIIEMETDKEHVHLLISCDPQYGIHKVVKGLKGFSSRVLRENFPHLKSSMPSMW
                     TNSYFVGTVGSVSLEVVKQYIENQQVRTKK"
     gene            37733..39169
                     /locus_tag="ACGI59_000339"
     CDS             37733..39169
                     /locus_tag="ACGI59_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HFG9738152.1"
                     /translation="MICTIKPLKISIKKSTEPLIEFISDTTYKNKSSSYIKEVYFTRY
                     IGVDDSIDKYIEKIKNIDTYFFNNPTIPYIRLNNLKINFDKEQTDKMLKIFNIYNSSK
                     FNSWDLYNIDFPCKIENDTLNWTKKIAFKNTLDLFSTSMPNCTSSIIKNFAVKLLCWI
                     DYFLPKLFYNKVKTTISPKIVYFGNIKRQELFFLYFLSQLGCDILYINPNKDILDLYP
                     ECSKFSTLFELSRKTPNILEIPFERHITNINYSENTNKTSPTTNKPNSKNTLNNTNLT
                     SKKIVQDEEVELSYEDLAKLSASVVMIVVCNNENKPFKSGSGVVINNEGYILTNLHVV
                     NDGYSFLVRFENDDKVYTSYQIIKYHSDYDLAVIKVDRKCKPIPVKVSKKPVRGQKIV
                     AIGSPLGLFNTVSDGIISAFRDFETVQMIQFTAPISSGSSGGALLDMFGNLLGLISAG
                     YDDGQNLNLAVESSLVKIFANNFIEIVN"
     gene            complement(39261..41135)
                     /gene="asnB"
                     /locus_tag="ACGI59_000340"
     CDS             complement(39261..41135)
                     /gene="asnB"
                     /locus_tag="ACGI59_000340"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HFG9738153.1"
                     /translation="MDGNISIYYKGDISKKEDIKFKIKESVNNTRLSSRIEYKDEEKT
                     FDFERFNVIDAIYGNKPFEKDGKVIVFNGNIYNYHEIKEELIKKGHNFTTNDDIEVLL
                     ASYIEFGKNCVHKIKGMFNFIIYDRENESIFGARDLFGIKPLYYINKENAIIFSSEYK
                     FLLEYMKNLNINERSLQSYFSFQYVLPEDTMIQGIRLIPAGHYFRVENGILSLKRYNK
                     LEFRSSTKFFYTKNHLGNRDVNEDDIRNIVVDSIVTHMEEEKEIGTFLSGGIDSSIIT
                     TVASQINPNIKSFSAGFSVKGYSELEVAKKTADKLGIENIQINITQDEYIKSLPNVIY
                     SLDDPIADPSEVGLYFLIKEAGKHVKVALSGEGADELFGGYNIYKEYSTMKSVVNSPT
                     YIKSILGRVSELMPNIKGRNYLYRATTPLEKRYIGNAKVFENSEVKRFFFKYKEKNIY
                     EYLLSNLYRDAQKNNYDYISKMQHIDVNTWLQGDILQKISKLSTAEQVELRVPFLYKD
                     VFDVAKNLRMEQKINKNNTKVLLREAFREIVPEHVVQRKKLGFPTPIRVWLKDSLGDI
                     VKETIYNSNVDEFIDKKYAIKLLDTHLKGHRDNSRKIWTIFTFCLWHQLFIEHKNVEY
                     "
     gene            41308..42012
                     /locus_tag="ACGI59_000341"
     CDS             41308..42012
                     /locus_tag="ACGI59_000341"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HFG9738154.1"
                     /translation="MKKKILIPVIMSLFIISQCITSFAFTPENNKFKVKPLPYAYDAL
                     EPYIDKETMKLHHDKHYQAYVDKLNAALEKYPELYNYSLCELLQNLDSLPKDIATTVR
                     NNAGGAYNHKFFFDIMTPEKTIPSESLKEAIDRDFGSFEKFKQEFQKSALDVFGSGWA
                     WLVATKDGKLSIMTTPNQDSPVSKNLTPIIGLDVWEHAYYLKYQNRRNEYIDNWFNVV
                     NWNGALENYKNLKSQD"
     gene            42125..42661
                     /locus_tag="ACGI59_000342"
     CDS             42125..42661
                     /locus_tag="ACGI59_000342"
                     /inference="COORDINATES: protein motif:HMM:NF016214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFG9738155.1"
                     /translation="MIKETHERGGYIFALLILPFINNLYLVKYDIAYKIVLIIIYIYF
                     AYLGSLFPDIDMRGSYISKKFMLIYKLFGSRFRHRGFTHSLIALLLIYSFFKSLTIFT
                     NNNIVFSCLSSGFIIGYLSHLCLDLITKEGIELFYPITINISLLPIKTNSKTEKFISK
                     LLNFIVIFLIGYQFYILL"
     gene            complement(42827..43447)
                     /locus_tag="ACGI59_000343"
     CDS             complement(42827..43447)
                     /locus_tag="ACGI59_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFG9738156.1"
                     /translation="MQYIQELLDLTQNYWVLATIIGLLSAFIESFIPALPLVAIVTAN
                     AAIQGLFIGCLLSWIGSGLGTTSLFLLISRFNDSKLFNKLRNSKTEKAISWMDKQGFK
                     LLFIAYACPFMPGCLVTIASAFGKKDLKDFVPAMLAGKFVMFIVISYVASDIEGFITS
                     PLKIASFILLVFLSWKIGNRVNKNLENHNYDFHHKKHHNDKDDKMI"
     gene            complement(43761..45899)
                     /gene="vanT"
                     /locus_tag="ACGI59_000344"
     CDS             complement(43761..45899)
                     /gene="vanT"
                     /locus_tag="ACGI59_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine racemase VanT catalytic subunit"
                     /protein_id="HFG9738157.1"
                     /translation="MIRNKSYAGLDYFRIIAALLIIAIHTSPLLSYSKMADFVLTSII
                     ARIAVPFFFMLSGFFLISTYNYNCNKLKSFLKNVLFIYGIAIVIYIPLNIYTGYFSMN
                     NLVPNIIKDIVFDGTLYHLWYLPASIIGASIAYFLVKRLGLKYSLVITIVIYVIGMFG
                     DSYYGFLEKIPLLKNLYGYIFEVSDYTRNGIFFAPVFFILGGIISNKSNYMSTKNSLI
                     GFLISFLLMLCEGMFLHKLGVQRHDSMYIMLLPCMYFLFTALTFLKGSRARLLRTSSL
                     IIYIIHPMMIVVIRMFSKIFGIQTVLVDNSLIHYIVVSIASFIASIIIILILKPLKKD
                     LKGEHKVNTNRAWIEIDLNNLEHNVKMIQKAMPEDCELMAVVKANAYGHSAFEVATCA
                     NQIGVKAFAVATIDEGIDLRRYGILGEILILGYTNPIRAKELHKYNLTQSVIDLNHAI
                     SLNKQKYNIKSHIKIDTGMHRLGFDVNDVRSILKAFKLKYLDICGIFTHLCVSDSLKD
                     EDVDFTNKQIECFYKLIDFLLKKGIKIPKVHIQSSYGFFNYPNLKCNYVRIGIALYGI
                     LSSPNDTTKLQLDLRPVLSLKSQVILIRKIQKDDSFGYGRVFIASRDSVIAILSIGYA
                     DGIPRNLSCGKSYVIINGCRAAIVGRICMDQLAVDITDIPNVEVGNTAIIIGRDNLSE
                     LSASEVANNSCSISNELLSRVGRRLNVIKK"
     gene            complement(45917..46723)
                     /locus_tag="ACGI59_000345"
     CDS             complement(45917..46723)
                     /locus_tag="ACGI59_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HFG9738158.1"
                     /translation="MMNNILLDKNKEDKMTNTILLTKDKVYDGNLILVNAFLPVKTSE
                     DIDLIPVDTRFPSILMKREATNILQNILKSICGINEIVPVSGYRTAEEQQDIYSSSLR
                     DNGKDFTKKFVALPNHSEHQTGLAIDLGLKKEVIDFICPEFPYNGICNEFRKAALHYG
                     FIERYQCKKEEITGISQEPWHFRYVGYPHSEIINKMNLALEEYIEYIKRFSYTNCLKI
                     EDKEKIIEIFYVPIHTKEEVRISIPEQCVYQISGNNKDGFIVTFWRNRKD"
     gene            complement(46720..47820)
                     /gene="vanG"
                     /locus_tag="ACGI59_000346"
     CDS             complement(46720..47820)
                     /gene="vanG"
                     /locus_tag="ACGI59_000346"
                     /EC_number="6.3.2.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-serine ligase VanG"
                     /protein_id="HFG9738159.1"
                     /translation="MNNKKIAVIFGGNSTEYDVSLQSAFAVLENLNTEKYDIIPIGIT
                     REGNWYHYIGDYNNIQNNTWFERDTDIIPVIVSQNRVKNGIIEFLIPDAVSQNHIPNS
                     AIESCENDIKYTKLDFVFPVLHGKNGEDGTVQGLFELAGIQVIGCGTLSSALCMDKDK
                     SHKLVHSEGIKVPKSILLKYSDKFNVAELSKDLTYPLFIKPVRAGSSFGISKIYKKEE
                     LQVAVDLAFEHDNEVIIEENIDGFEVGCAVLGNDDLLVGRVDEIELSKGFFDYTEKYT
                     LKSSKIHMPARIDLDCEKRIQETAKIIYRTLGCSGFARVDMFLTPSGEIIFNEVNTIP
                     GFTSHSRYPNMMKGIGLSFEEILDKIIEVYIK"
     gene            complement(48081..49163)
                     /gene="vanS"
                     /locus_tag="ACGI59_000347"
     CDS             complement(48081..49163)
                     /gene="vanS"
                     /locus_tag="ACGI59_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS"
                     /protein_id="HFG9738160.1"
                     /translation="MRTALMAFIAIIFIWILYSFILYGKFSIWIVSFFENAFRMEYKA
                     ALNLYQQIFRNSMELFMILAMVIVFFIIFRVYLNWFTKYFSEINQGIDSLIKEDVGEV
                     ALSPELLAIEKKINSIKHILEQRKFETQMAEQRKNELIVYLAHDLKTPLTSVIGYLTL
                     LRDESQISEELRKKYLSISLDKAERLEDLINEFFEITRFNLSNISLEYSTVSLTRMLE
                     QLTYEFKPTLMSKNLKYTLNVTPNMMIRCDVNKMQRVFDNLLRNAVYYSFEDTTIEIT
                     AVQEDDYIKLKFINHSNTIPEEKLERIFEQFYRLDTSRNSNSGRAGLGLAIAKEIVEL
                     HNGTITAHSENNIIIFKVIIPIVGNL"
     gene            complement(49213..49914)
                     /locus_tag="ACGI59_000348"
     CDS             complement(49213..49914)
                     /locus_tag="ACGI59_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-G-Cd"
                     /protein_id="HFG9738161.1"
                     /translation="MNENILIVDDEKEIADLIEVYLKNEGFTVYKFYTGKEALECIES
                     KNLDMAILDIMLPDIDGFHICQKIREKYYYPIIMLTAKIEDIDKIMGLTIGADDYITK
                     PFNPLEVVARVKTQLRRYMRYNNSYEQQSIIVNEYDIKGLIINKETHKCSLFGKEVAL
                     TPIEFSILWYLCEHQGKVVPSEELFEAVWGEKYLDNNNTVMAHIGRLREKIHEPSKNP
                     KFIKTVWGVGYTIEK"
     gene            complement(50348..52879)
                     /locus_tag="ACGI59_000349"
     CDS             complement(50348..52879)
                     /locus_tag="ACGI59_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFG9738162.1"
                     /translation="MSLKSLKIKENLYWVGSLDPDLRVFDIIMYTPYGTTYNSYVLKG
                     TEKTVLFETVKDKHFDNYIERLNDLNIDFEKIDYIVVSHTEPDHAGSVEKLLDLAKNA
                     KVVASETAIKYLKEIVNKDFEYVAVTDGDTLSIGDKTLEFFSVPMLHWPDTIYTYIKE
                     DKTLVTCDSFGSHYSNDKIVNTLDENEEKDYLDALRYYYDCIMGPFKPSMVTAIEKIK
                     DLDIDTVCPGHGPVLTENPRKIIDLYYNWSVNEQIKLEKEVTICYVSAHGYTKIMAEA
                     IKAYIEKNSNYKVNLFDVIEHKQEDILAKIAVSQGVLFGTPTILGDALKPIWDILISL
                     NPVLHGGKVASVFGSYGWSGEGIENAMERISQLRMTAVKPFAVNFKPSNEEIDKLYSY
                     TGKFLDKLNSTFGSKKKTKKFKCVICNEVFEGDSAPSVCPVCGAKEDQFIEVEEDEVT
                     FRKDTDEYFVIVGNGAAGFYAADAIRKRNKTCKITMISNEDELTYYRPALSDGINEEL
                     GSDFYMEDKDWYDKNNIVVILGTNVDKLDEVNKTIIVNDGAIKFDKLVIATGSRNFIP
                     PIKGHDLENVFTLRNIKDLYSVKEALEKSKKVVVIGGGLLGLEAAWEFRLKGLEVVVV
                     EAMDSILSKQLDKEGSKILEQCVRDTGIDVRLGVAVDGIEGDGKAQKVVFKDGDSVDC
                     DMVVFSIGVRANTQMVQDTSVKIDRGIVVDKTLQTNVKDIYACGDVAQVGNISLAIWP
                     SSVEMGKIAGANASGDNLTFESEVYPVSLDAMNVKVFSIGNIQNFDKEISSKDEGQRI
                     YKKLFMKDGSLVGAILINDLSCTVKLIRLISEKGDFEDIMKADIL"
     gene            complement(52987..53604)
                     /locus_tag="ACGI59_000350"
     CDS             complement(52987..53604)
                     /locus_tag="ACGI59_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFG9738163.1"
                     /translation="MCKKTYLLVVSMLIAGLLTACSGSPTKDSGQAQDNTKKEANASN
                     NALKDEKNNENLMEQDFKVPYTDAINIFKDKYKDADIVDLSLERDLNKFVYTVEGVDD
                     NNEYKMKIDANTKDVLEDKTEKLDSEDLNGVARKEKLDLNDIMTPQQAMEIALKEQNG
                     IVKEWSLDKDLDVTFYKIRIDKDKNEYDIKVDSKKGTVLKVEKED"
     gene            complement(53966..54850)
                     /locus_tag="ACGI59_000351"
     CDS             complement(53966..54850)
                     /locus_tag="ACGI59_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HFG9738164.1"
                     /translation="MDTTKIDDISKLDLNNLDINKMDMGGIMESLLEWATTSGVKLII
                     GILILSIGFKIIKKFVNHVMLLLSKRDIDLTLRKFLKSLLLSVLKVAVIIIVLEYWGM
                     SLSSFAAVIASAGVAIGLALQGSLSNFAGGFIILLIRPFKVGDYVEAAGHGGTVEQIG
                     LFYTQLVTPDNKQILIPNGSVSNDSLINYSAKNTRRVDLTFSVGYEDDILHVRRVLKD
                     IVNNHKLIINEPEPFIGVVEHGDNAIKFATRVWCKTEDYWTIYLDLLEEVKVRFDEEG
                     ITIPYPKMDLTVKELNKI"
     gene            complement(55041..56291)
                     /locus_tag="ACGI59_000352"
     CDS             complement(55041..56291)
                     /locus_tag="ACGI59_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFG9738165.1"
                     /translation="MKSYTKAKWSVWGIITFSFVLVLFLRMSTAVVSDNLANELGFNS
                     IQISNIASFCLYAYAFMQIPAGILIDKYGARKISSLGIIMASLGSILFGLIQSIELAY
                     ISRVIVGAGTSVILLCILKIQGRWFNKSEFASATAKFSFVGNLGGVLATFPLVFLSEL
                     VGWRNSFLLIGIIGVVIGCFMYIIVRDTPKEYGFNVDTEPYEKSEKVNIVDGIKSVIK
                     NKSTWYNSMIMFSFVGLTSAFISLWGVRYIMDVYGVSKSFSAFIVSFFTYGFIFGSII
                     MDFVFAKIRSSKFNIIKFGAMIDLFIWIVIVVVYQVKPPIIVLPISFFIMGCIVMSHL
                     QVFNDAKYKNKEIYSGLATSVINTFEFIGSGIINLIIAISLQVNSYNIVDGYKKGFVV
                     FIVLSIITIVSSHIGVKNDDFKAI"
     gene            complement(56513..57277)
                     /locus_tag="ACGI59_000353"
     CDS             complement(56513..57277)
                     /locus_tag="ACGI59_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9738166.1"
                     /translation="MLGKLLKYELKASGRIFIPLYIAILIVAVFNGIFMNTNILQVQG
                     IGILVLTSLFMALGVLTIVVTIQRFRKNLLGDEGYLMFTLPVSTSSLILSKCITALIY
                     AVLSFIVAVFTFGVLMLFGTSGILLPEILDLFNTSFKWISENFLDILLLVVVMFISYS
                     SFILLLYTSISMGQLPKFNKHRNIVAFASFIAINIVISIVGDAVGSILPNEDTNMVYH
                     LYQSPSFMLAILGSLVVAIALFFATKFILDKKLNLE"
     gene            complement(57271..57996)
                     /locus_tag="ACGI59_000354"
     CDS             complement(57271..57996)
                     /locus_tag="ACGI59_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9738167.1"
                     /translation="MDKNISNVSQDGIVEFKGVNKSYGTKNVLKNIDLNIPKGKIVGL
                     LGPNGSGKSTMIKLMNGLLQPDNGEIMINGMKPSIDTKKIVSYLPERTYLNDWMKVSD
                     LLKFFYDFYSDFDVRRANEMIKSLDIDVNEKLKTMSKGTKEKVQLILVMSRNASIYIL
                     DEPIGGVDPAARSYILKTILKNYSEDSTLLIATHLISEIENICDEIIFISKGEIVLQG
                     DVEAIREEKGKSIDALFREEFKC"
     gene            complement(57998..58366)
                     /locus_tag="ACGI59_000355"
     CDS             complement(57998..58366)
                     /locus_tag="ACGI59_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFG9738168.1"
                     /translation="MEWELDNNKPIYIQLVEHLKLKIISGEIKIGSKLETVRALAEDA
                     EVNPNTMQKALTELERQGLVYSQRTKGRFVTDDKEKIKAMKEEIANVEINTLKVTLEK
                     LGYDRDEMLKLITENLKGEL"
     gene            complement(58542..60086)
                     /locus_tag="ACGI59_000356"
     CDS             complement(58542..60086)
                     /locus_tag="ACGI59_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HFG9738169.1"
                     /translation="MRNLQKKLMFFLFICIILQITYTTPVYSLCKLYKNNSINNFESI
                     VSNNQLISKHAIQIILIMAIICVILIFYIIYDKLNFKIKLQRIAYTDNLTGANTIDKF
                     VIDANKILCKNTQVKYALLYIDIDKFKYINDLFGYEVGNEILRNLTKIIKSNIFEEEM
                     FARISADNFIIIMKYIEEEDITKRLKTIFEELDLFNNNQEEKYKLVLSCGIYFILPED
                     RDINSIIDRANIPHKMAKGGHKSSYAFYDNKIHDQEIKEKEMENTMFSSLENKEFIIY
                     LQPKIELNTGEIQGSEALVRWKRPDKGLIPPNEFIPFFERNGFVINLDLYVLEEVCIY
                     LRKWIDAGINPVTVSVNVSRIHLYCNNFIETYKNIIDKYNIPAKYIELELTESIIFDN
                     FDILIDIMNNLKKIGFLISMDDFGSGYSSLNMLKEIPMDILKLDQKFIMETYNSKRSK
                     IIVTKVIEMAKELGMKVISEGVETEEQFKLLKEVKCDMAQGYLFGKPMPIEEFEHLIV
                     SNLVRG"
BASE COUNT        19363 a        10666 c         6158 g        24077 t
ORIGIN      
        1 tttaatattc atttataatt ttaattatat aactaacttc ttcatcttcc ataccaataa
       61 ataaaggtat actaacaact ttatctgaat agttctcagc tatattaaaa ctcccctttt
      121 tatatcccaa ataagcatat gcttcagata gatgtggtgg tatcggataa tgtattatag
      181 actctatacc acttttcttt aagtattcca taaatgtatc tcgtttttca acttgtaaaa
      241 caaataaatg ccatacatga gtacaattat tttctatctt tggaagtatg actttgggat
      301 tattaatttc attaagatat ctatctacga tttttgattt ttcaatttca atttcattta
      361 gatatttaag ttttactctt aataaacctg cctgaatttc gtccaatctt gaattatatc
      421 cgacaaattt attataatat cgcttttctg agccatagtt tctaagaatt ttcactttat
      481 cagctatttc ctcattacta gttactatag ctcctgcatc tccaaatgca cccaaatttt
      541 tacttggata aaaactaaaa caacctatat caccaaatgt tcctgtcatt tttccatcat
      601 atttagctcc atgagattga gcacaatctt caattaaata caagttatac ttatcacata
      661 attcttgtat atctttcata ttacttgctt gaccatataa atgcactact aatatagctt
      721 ttgttttgct atttatcttt tcttctatct tagatgaatc tatattataa tattcatttg
      781 gttctacaaa tactggtgtt gctccattta tactgattcc cataacacta gcaatatatg
      841 tatttgattg cacgattact tcatcacctt tgcttattcc taatgctcta aaagcaatta
      901 tcagtgcatc taaaccactg gctaaaccaa cacaatattt gctacctaca tagttagcaa
      961 attcttcttc aaaatgcttt acttcatttc caaggatata atatccactt tccattacat
     1021 ccttaatctt ctgttcatat tcttctttat gagtttggaa ctgttttaat aaattattaa
     1081 atttaaccaa caataaattc acatcctaat ctatctattc tctatgtggc tatcttaaat
     1141 ttatttaatt aagaaaatac atcactaatt gtataataac tacacattat ttaaatactt
     1201 taaaaattct ttatagtctc taatataatc ttcttcatta taatgttcag atgctataga
     1261 aattaaaaca gttttttcat caaaatcaaa catctcatgc cacactcccg ttcctatata
     1321 taatccaaca tcatttttat gtaaattaaa tatagtctct tttacaccat cataacattt
     1381 tatcttcaca cttccattta ggcaaactag tacttgttcc aagtttttgt gtgcatgaaa
     1441 tcctctcttt acattgtttg gtacatcata tatgtaataa actctcttta tatcaaaagg
     1501 tattacttta tttgattcta atgcaactaa atttcctata tattcgtttc caatagatac
     1561 gaacctaatt aaattatatt tatccataaa tttatctatc ctattcatgc taattttcta
     1621 acaatatatt acttcatatt catatttaca ataaactatt gtaaatatga atgaaataaa
     1681 ctatatccaa tatttttctt ttggttcttt taattctatt tcttcacagc ttagtgtttc
     1741 acacaattcg taaattttat ttgcaaataa tatatcatgt attgacaaac ctatattgta
     1801 agatattatt ctctcatcat cattttctct tcctttagct tgtttactta aaacatcata
     1861 aaattcacaa gaatatttat atctatctat attttctaga tgtttcaaat gattataatc
     1921 atctaaatat actttatcaa atagataatc aacatttcca aatcccataa catgtacagg
     1981 taataataaa gcaccttttt taaaccattt aaattctgat aaatattcat ctgtatatgt
     2041 cacagtagaa ataactacat ctgaatctac aataagttct tcatgtgtat cacatatttc
     2101 aaaactaaat tgttcatttt tttcatactt ttttacaaac aactctgctt ggtctttata
     2161 tttaagtaac tttatattta tatttttatc tttaagagta tatgaaagca tattcattat
     2221 agcagaagca gttacaccta aacccataat agcaatagta ttaaaatctt ttttagcaaa
     2281 tgtatcgata gccaaggcag caacagcagc agtcctaaaa gctgtaatat aattaccatc
     2341 caaaatagcc tttaatttag catcttccat attgtataga aatatctctg agtttaaact
     2401 tggatttctc cctggcaatc ttgtaaccat ttttattcca ccaatcatat gattatgatt
     2461 tagggatggc attacattgt aaaatacatg tccttcttct accatgctta tttttggtgg
     2521 taattgacaa tctttttttg ttaagaaaga ttcttttatc cactcatatc cttgtttctg
     2581 agaaatgttt aatgacataa tttgttcatg agttattagc ttcatatcta tcatactccc
     2641 ttatttaaaa attttctatc atattttcat tattttcatc aagtatttct atcatttcat
     2701 gaatacattt cttttttata tttgcatcaa atatattatt tgctattcca taaactcttc
     2761 cacaatcctg tctctcatct tcatataatt ctattgtatc tccttctttt actagtaata
     2821 gaaattgagt gacataatct aagttttcta ttttatctat attatttatc ttttttattg
     2881 tcccgggtct tatgtatatt ggtaatgcat atacacattt tgatagatgc atattatatt
     2941 tctttatttc atcaaaattt acttcattat tcacactaaa gtttgcaaat aaacttccta
     3001 tgtctaaatc tgttatttga gaaatagcca tatagtcaca acctccaggt aatcttggca
     3061 ttacttcaga tacctttatt ccatatgctc ccactaacat ttgtacataa agaggtccat
     3121 tttttatatc aaaagcctgt gttatatttt gatatataga ttttatttcc tcctcatatt
     3181 gaaaacaata ctttgatgga taaagtaatc tatctggttt tccagctctt cctgtatgta
     3241 tgtttacaag ctctctatct ccaatataaa atacatgtga ctctccatta tgaacccaag
     3301 ctagtccttg tatttcctca ctttcataaa attcctcaat tattacttgt ccatcttgtg
     3361 aggcttcctt agaatattca aatttttcta ttaattcttc tatatttgat acaaaatata
     3421 cacctcttga accataacca tcaacaggtt ttattacaag tggaaaattt aatccttgta
     3481 ttttttctaa atcttttaga ctttcaataa tacagtaatc agttgtaggt attgcattat
     3541 ttttaaaata ttttttcata atagccttgt tagttacaga ttcaagttga ttgcaagaaa
     3601 tcacacatgg taaatttagt tcatttgcaa tataaacata tggtttcatt aaagaatctg
     3661 taaatccagt taaaattcca tctatctttt tatctatagc tatacttttt attttatcaa
     3721 aatcaaatac atcaacatct attgcttcat cacaaatctt tttcgcagga gtatcaattc
     3781 tattatcaca aacaatcgta tatattccct cttttttagc tgatataaca atattctcaa
     3841 aatacttaat tgagcctaaa atcattaatc ttttcatata caaaccttct ttataaacta
     3901 attattttta tgtaatgtta gagtactaga tatttactat attctaaaaa ctctataaaa
     3961 catgattctc acttatttat atttttatag tatgttctgt tacaaattgc acttgaacca
     4021 aaaccttctt taaatgtagc taaaccctcg tctaagtatt tgcctctatt tagagtgcta
     4081 attccaaaag aaagatactt aaaatttctc tctaaagcat cttttattag attatagtat
     4141 aaaaatatca ttggataata tttattatat gtcgaatttg atgctagata ttgagtatga
     4201 taaactcttt cttgaaagtt aaataccatg ctcccacaaa tcataatgtc atcaatatat
     4261 actgcataaa attctacaat atcttttaat ctattatttt taaactccaa aagttcttct
     4321 actgaatgta ttactttagc tcctttacta tctaaacaat tagaaagtaa attaaaaaaa
     4381 caaactatat cttcatcact atacagtctt ttaaaggtaa gattattctt caaagaatat
     4441 ttaaaatctc ttcttttact gtatttaaaa ttggctaata tatcttcttt atacttactt
     4501 aattctaaaa acatacttaa ttcatcataa aacatgtaat tattcttaaa taaaaaatat
     4561 tctagtaatg aagtatcctc tttacaaaat attcttgatg tattttttaa atatacttta
     4621 tcgaaatttt gttcccgtaa atatatttct aacttatttg taatggcatt tacatgccta
     4681 atatcattaa aaccacttct tattataata ccaccaaaag tactccctaa atgagaaaaa
     4741 aatattcttt caccatcttc ataagtttca catgcaggta cgactgctag tatttcttca
     4801 cctttacata ttattaatga gtggtcagtg aattttccct cctcatgata attaaggaaa
     4861 tttctagttt gcagaaatgt accatttata gatttatttt ctacaaaata atcccagttt
     4921 ctttcacagc ttttttcaaa tttattgata tgaatattgt tcattttacc acctatgaca
     4981 tgtttttatt ttttaccata tagccatata atgctacaaa aagctgatag tcctcgattt
     5041 catcattttt atataaatca tacttaactt tatgatgatt agcttcatta tatattttgt
     5101 ctatatagtt ttttatttca atacagtgtt tatctataac aaaatgtttt atttccgatt
     5161 gtttttcttt tatcctatcc ctgtcaaatt cactagtatt gtaccttttt atcatatcta
     5221 taacctcttt tatattctta taatcaaact tatctataaa attccacata tttgtaaaaa
     5281 ttggtgaaac ttcattttta aacttaacag gaattaaatc atataaaatt gcatctagtt
     5341 ttgaagtatg acttcctacc atgaaagact ccatatatat atccgaaatc ttgtaaaaat
     5401 cgtctatatt tgctgttgtt ccaaaacata taactctatt atctgtaacg ttacttaaag
     5461 ttttccaata gagattctct gtatcaactc ctacaacaat aataattgta ttatctgtat
     5521 tttcaatcaa tgttttagca attttaaagt aattaatatc atttacacta ttgtattttc
     5581 tttcactagc aatagtcaat attattgtca catcatcctc taaattatat cgttttctaa
     5641 cttcagattt tgaaaatact tctttttctt ttaatggcat tggtaatatt tcagatttat
     5701 ctatgcctct tttatctaat gtaattaatt gactagcttc gctaaaatca atgacaatat
     5761 ctgcaatact tgtccctaac caaaaagtac tatcatagtg attaacatgt aaaataggta
     5821 ttttcccatc aactccaaaa gcaacaacag gtattgtatc ccacatatga acatataaaa
     5881 taactatatc agcccagtca taagctattt tcctcaaagc tacagctctt tttaaataat
     5941 catctgaaat taaatccaaa gatataactt cgccaccact atcatttatt gcttttaata
     6001 accagtctgg cagtgtatct gattgccatg tagttacaac tgaatgaaca gagtctttgt
     6061 caatttcaat ccaattttta actagtcttg tatgacctcc tgttgaatat ccttcactta
     6121 aaacatgtaa tacttttctt ctttttaaat ctttttttgt aaataatttc gtattttcta
     6181 tttctggtaa attatttgca cattctagca atgctctctc acattcttca caagaataca
     6241 aaccagtatg attaaaaaca gaaaaatgag caattttaga aacaatacct aaaacatcct
     6301 tgtaattctt taatcttaat tgttctgtaa gctcctcttt catacaatcc attacatgct
     6361 tattactctt aagtatggta cttgctttct tcttatcaaa ttcataaaaa ttttctctat
     6421 ataaatcaag atttatgtac tcttttacaa attttatcct atattcatca tatccattat
     6481 ttatatttac tactccagaa tcatgcatac accaaggctt attttgtttt ggtacaataa
     6541 ctttgtattt attttgtcta aactccatac tctgtgacaa atcataaaaa tgccacccat
     6601 caaacttatc ctctctccaa aatacatcat attgagtaat cataattaat ccatctatag
     6661 ccataacttc attaaaacat tcttcaaagt tactaaattc ataaatatcc attttacctg
     6721 tatgactatc atatactttc cctgcttttt tatcagaatc ccaccaaatt ccattcacag
     6781 gcatatcttt tactccagct acaccaatca ttccgacatc tgtatttttt ttaaatatct
     6841 ctaaaatatc atgtatgaaa tttttattaa ttataaaagt atcttgatgt aagtatacct
     6901 tatacttaga atctgtttct ctcatagctt tattataagc agaggcaata gaatcggctc
     6961 ctcttaaagc tactgtttct atactaaagt ttttaggaat gtctaggcta tttatatata
     7021 caagacattc ttcataaagc ctttcatcat ttacacaggt tataaaacat atcttatcat
     7081 tattcatatt tcttcccctc aataaaacaa agtatctctt gtatatcact ttcactctgt
     7141 actttttcgc tactattttt aattaaatta taagcagtat caaattcttc taattcacat
     7201 aatacacttg ttatattata tattgagtca atatcatctc tatccatctc taatactgtc
     7261 ataaacatgt ttatactaaa atctcctaga cctctgttaa atgcctcaac tcctaatcta
     7321 tttaatagag ttgctttatt tatcacaaat gtatcaatta gatattcaat atcatcaaca
     7381 aaacatggtt cagttatatt ataattatca aatatataac ttaaattact atctacatct
     7441 aattcattat caattctcat taaagcgtat tttaaattaa tcatactaat actagaatat
     7501 cttttagtat ctaggatttt agaggctttt actatatatt gataagattc gtactgttgt
     7561 ctcatttctt tacagctttc cttacatatt gtatcaatga agctattatc aatttccgaa
     7621 agtggcatac tagtagctgt aatactatta attatgtaat tactatcatt aaacattttt
     7681 gttatctcat ttaatgtaaa aaaccttaaa tgagtcttgt ccaaaattcc tgaatcttca
     7741 tatgtaaaat tacctaacac aagatttctt aaaacagaaa catgcataat atttggtata
     7801 ctagatacca gataaccatc cttctttaga tattgtttca attcattgac tatcttccat
     7861 gggtcaatca aatgttcaag tacatctccc aatattatat aatcaaaaaa atctttttta
     7921 taatccaatt ttactttttc tatatttgct ataagtacat ctgcactata ctttgcaagt
     7981 ttacctgctg actcactaat ttctattcca tatattgatg catttttata tatatttttt
     8041 atttctaaaa gagtagcccc tataccgcat cctatttcta aaacattaat atttgcctcc
     8101 aaactatcat ctattttaga aatcaaatct gttcttatca ttccactata ttctggatta
     8161 aatccccact tagtttcaaa ttttcttcta ttaatataca ttatatctgt atatttatta
     8221 cgattttctt taaaagacac acttccaaaa tggtgtataa aagtatcctg gcataataat
     8281 aacttatatc ctgactctat catcctaaaa gataaatcat catcttcaaa atttccaggt
     8341 gtgaataatt catctaaaac ccctacatta tccagaacag ttttttttat taacatgcaa
     8401 aatccaacta acttcaatct atatttccat aggttaatat tagatttatt tattttgtgt
     8461 gcaaatataa acatgtcctc aattgactta taatcaacat caatttgctg ataatatgaa
     8521 catgcattcg taataggtcc tacagcacct atattatctt gagaccataa agctttattc
     8581 aaattataga gccaatttgg agtaactatt acatcattat ttaacaacaa tatgttttca
     8641 ccagtagcta actcaatacc ttgattgcat ccttttggaa atcctaagtt tttgttatta
     8701 tacacagatt ttatatcaga ttgtcccttt aaccactcaa ctgttccatc agaagagtta
     8761 ttatcaatta ttattatttc atatttattc tttggggtaa atttcctaat actttcaata
     8821 cataatttag tatattctat ttgattatat gtcaatatta ttatgcttgt aaattttata
     8881 tcttcatttg tacatgtata ttgccaatct tttattatgt ctttagattt ttcattaacc
     8941 actattggta tattcattct tcaattctcc tatttttgac ttatcttaaa ctttttaagt
     9001 attctatatt atatatagta tcttcatttt ctacatctaa tgataatgcc ttttttaaca
     9061 taaaatcagc tttatcaagc ttattttcaa acatgtataa aattcccttt atatttaaaa
     9121 tctttatatc atccacaaat gtttttgaat agtcatctac caataatttc gcatcattta
     9181 ttttgccagt ttcaatcata ttttctatac taataataaa actatctttt aaatccttaa
     9241 attcttcact ttcttttata ttttcttcta attcacctat aagtattttt attattttat
     9301 tataaaaagg atactcattt agtaatttcc tcatactatg tatatactct aatttttcat
     9361 tagcattatt tactttattt ttaatatcaa gtagatgctt aaattctata gtgaacttat
     9421 ctaaatcatt acaaacaaat tttattaatt caaaatcact taaattgcta tatatgtatc
     9481 ttatgtactt ataagaatac attatataaa gataaaaaat ctccttatat ttttcatcac
     9541 ttaagcttat actttctaaa actactttag atatagcttt atatactctt atcttttcca
     9601 tatctgatgt atttggacga ctaattatgt acttatatag tactaaatta aaacttcttt
     9661 ctaatgctaa aagatatttt agatatttct ctatccatac atattccatt tcataaaaaa
     9721 tatctagtaa atcaatcttc tcttcaaaag ctatttttat aatatatgca taaattattt
     9781 ccttttcttc attgagtatt tctttacatt tatctataga tacacattta tttatcctta
     9841 tttgatttaa aatgccataa ttaccttcaa tgtcacaaag gattttatat acatactctt
     9901 tttcatcctc tttaatatta tttataactt cttctatatt tctataaaaa ctattttttt
     9961 caattttaga atttgaaact tctttataat aattttttat atcttcaaat ctattcaatt
    10021 tatatagtga cataaataag ctaaagtaca cattcttttt cttttccaca tcttcaatgt
    10081 tattatactc tgaaattaca aactcatatt tttcaagctt ataataaatc tttatcagtg
    10141 taataatagc ctcatctcta agccctacag tatcagtgtc tgaaaataaa ctatttgctt
    10201 gtgtacttat ttcataatta tctaacaagt aaatatatct cttatacgaa tctatactat
    10261 tttcatactt tcctaaatct acttgtgctt gtgcaatata ataatatata tcaatattat
    10321 tcatgtcttt cttaatatac tttaagcaca atctttcaca cttcttatgt tttcctaaat
    10381 ataaatatgt ttttacaagg cttgttatca agtaaccagg tgccaattct agtttagaat
    10441 ataattcata agatttctca agatagtata aagcctcctg atattttcct aatataataa
    10501 aatttttccc taattgatag tttgtatatg gagaattatc attctctttt aactcttgaa
    10561 ataatagttt ttcatttctt ttcaccttat ttaatcttat ttcttcatct tcaaacaaat
    10621 atccataatg ttcaaaatca gcaacattat taaatatagg gtttttgtat attggttgtt
    10681 catgtacttt accatggtat ctaaaatcaa cgtttttaaa aagtctaagc actgaacaat
    10741 atccatataa ttttttatca tatgagtaaa gattttttaa ttgtattgaa gcagaattaa
    10801 attttttatc aagaccatct ttaaaaaatt ttacaatagt tgaagaatca attagtattt
    10861 catctgcatc taatataagt aaccattcac ctttagcata ggatattgat atatttctca
    10921 tactagaaaa atcattattc caattatgaa aatacacttt ttccgtgtac ttcttagata
    10981 tttttatagt atcatctgta gaacctgtat cgactattat aagttcactt tctatagaat
    11041 ttcttaatgt ggttaatgat tttaaagttt tttcaagtat tttttcttca tttttaacaa
    11101 tcataactat actaagtaac atataaattt tcctttcaaa agtatatttg taaaaaaaga
    11161 aaggagtaaa gcaaagcctt accctttctt ctatatccaa tatacaaaat tattttatcc
    11221 taataattgt aaaactcctt gtggttgttg attagcttga gcaagcattg actgtgatgc
    11281 ttgaactaat atattcattt tagataagtt aaccatttct gaagcaacat ctgtatctct
    11341 tattcttgat tcagctgctg taacattttc tatagtatta tttaagttgt tttgagttga
    11401 ttctagtcta ttttgttgtg cccctaattt tgctcttgat gtatttaatt ctttaagagc
    11461 tacatctaag ctagatacca tttgtttagc tgcatttgtt ccagaagttc catctgcatt
    11521 taagcttgct attgtagaac ttgttatacc tgctttactc attatacttg aagtatttac
    11581 tagagcaact tttatttcat tgttgttgtt agatgttcct gcaacatttg ttccaaagtt
    11641 tgctccaact tgtaatctta tttctgtaga acttccatca agtaatttct taccattaaa
    11701 ctcaattgag cttgatattc tttcaacttc atcttttaat tgtagtaact catcagctat
    11761 cttttgtctt tcttcagcag tattagtttc atttgaagat tgtactgata aagttctcat
    11821 tctttgtagt atgttaccag tttcttctaa tgcaccttct gctgtttgta caacagatat
    11881 accatcttga acgtttcttc ctgcttggtc taaacctttt atttgagctc tcattttctc
    11941 agatatagca agcccagcag catcgtcagc agctctctta attctaacac cagaagatag
    12001 cttctccata gacttacttt gtgcatttac gtttcttccc atttggttat ttgctatcaa
    12061 agcacttaca tttgtattaa ctctcattat tttaccctcc ttagtatagt tgacatcctt
    12121 gtcatttcta cttttaatct tttttattaa ttcttcattt atataatcgg acaagatggt
    12181 acataacttt atactttttt attaaaaata tttacttttt ctctatttaa gttataatac
    12241 attgtgttca cttgtttagt aagcttatac tcatgtaatt ctttttttac ttctaataat
    12301 tgtccttgta aaatatctct tatcctgcta tcaatttcaa ttaattccat tctattataa
    12361 agctgtataa aatcatttct atcaatttca cttacactat ttataatctc ttgtctttca
    12421 cctaacttaa tacttatctt tatatattct tccttttcaa tcaaatttat aatttgtaaa
    12481 gatatatctt tatataaatt taatttttct tttaatttat ccataattaa ttaccctgtg
    12541 cttgtgagaa ataattcatt tgggaagaat actgattcaa caaagattct aaggctgaat
    12601 atttagcata aagtcttttc tctttatcat tcattttttc aactaacctg cttatatttc
    12661 tttcttgctc tgcaatttgt tttgaatata cattttcact agcagaagca gttttttcta
    12721 tacctgcttt tttaggaagt ttagaagttg aacttccaga atattcatat acaatatctt
    12781 ttagcttttc aaacattccc ttatcatcat aattcgaaga ataacctgct agtgttttgt
    12841 ataccttatc actatcatct ataagagcct ttgaaaaagt atctgcatta aatgatattt
    12901 gacctttttt attgtaattt tcactttcat tgatacctag ttttcttaat gcaataatat
    12961 tttttccatc tccaaaaaat gcagattgaa tatcttcaac aaaacctctt aactcatcat
    13021 catttctaag tataccttct ttagctttct tttcccattt ttctatttct tcagttgtca
    13081 tatcttcttt ttgggcatct gtaagaggcg gataatcttt tggtttttta gtagtaacta
    13141 aaccatagac cttgtccatc agtttattat aatcatccac aaatgcttgc atatttttaa
    13201 cagcttcttc agtatcagtg acagaagtca actttgcagt tccttttgaa tttacattat
    13261 agtcaatatt atctatatta aatgaatttt gttctttact tatatgagtt actcttccat
    13321 gttcatcagt tacatcagct tctagattct ttccatatac atttgccact ttgtttccac
    13381 cagcatcatt tataaatgtg ataccattat cattagcaat ttgctgagct aaattgtcat
    13441 ttccaattac tcttaaatta atgtctgatg acgaaccagt ctgttttctt gaaataatca
    13501 attcacctgt catctcacta taagaagcct ttatatcatt atctgcgcaa agggagttta
    13561 tcttacttac aatagtactt gtagtatctt gttcagatat tggtatattt acatctccta
    13621 tagttaaaga actcgctcca ctggcatttg gagggaactg tcgaacaata ggttcacttg
    13681 aatttaattt aatagtaaat tttgctggtt cagccatttg agaaactgca aattgataat
    13741 ttattttttc tgcaccagct gaaccttttg ctactataat actttcatca gaactggtta
    13801 ttcttgtaga agagtatgct ttttcactta ctatactatt aggagaattt atgcttagat
    13861 atttatcata aagatctttt gattcttgta taacatttct atatatttct tgcttccatt
    13921 taacgatttg ttgttcttgt tttgctttat caactttttc cttgtctcta atcatactag
    13981 cttcgattat gccttccata tcaaaatttc ctgaaagacc tgtaactctt ataggactta
    14041 tacttgacat acctttatcc ctccatctaa ttatattttt agttgattgt aaacttcatg
    14101 ccatgtatct ctaatttgtt ttactattgg caatatatca tcaattattt taatatcttt
    14161 attttcatca gctattgcta attcagattt tataaattca taaactttat acatatcctt
    14221 tgcccattgt cctgcatttt ggtctaatgt agtcattagt tctgtaaata tattttgaac
    14281 tcttacaagt tcattatgtg ctttcaaata gtcgttattt aaaagagctt ctttggatat
    14341 attggtatat tttactgtgc catctacaag catcaatagt aattcctctt ttgataactt
    14401 atctaattca gctaaattta attccattat ttgtacctac ttctttcttc tttacttttt
    14461 ttatcagctt catgccatgt atctttaatt tgttctatta caggtaacac attatctatt
    14521 acctggatgt cttttctaat attggctctt gaaagttcat atcttacaaa atcataaact
    14581 ttgtacatat cctttgccca ttgtcctgca ttttggtcta atgtaaccat tagttctgta
    14641 aatatatcct gaactcttac aagttctcta tgcgctttct gtgtattttt atctaagata
    14701 gctgctctgg ctattttggt atattttact gctccatcaa caagcataag taacaattgt
    14761 tcctttgatg ccataaaaac agcattttgt ttataagaat tataaggatt ttctccatac
    14821 atatattact tcctccaagc ttattatttt aatgacttta aaatttttat atttttattt
    14881 gtagctttta tattctcact ctttactttt tcgtatattt cttttcttaa tatacttata
    14941 ttgttaggcg ctgatatagc tagttttaca ttgtttccat ctactcctac aatttttact
    15001 tctatgcttt ctcctataag tactgcttca tctttttttc ttgaaattac tagcatccat
    15061 tatcacctct caataatgga tgccttattg aatatttatc atcctgtaaa attatttgca
    15121 ttccaaaatt agtttttata tttatcacta taggagcttt catgttcaca gttgattctt
    15181 caaatgtctg accaagagta attatattaa ataatacaac atcctttgga gatttcactt
    15241 ctagtttttc tattgtttcc tgatttaaaa ctatttcata ttcattgata atctcaaatg
    15301 gagatacaac aacaaatcct ataccttctt cttcagtaga attaaattct tttaaataat
    15361 cattatcttc tatatctaaa agctcatatt cttttaaatt ttcaaaacct aatatacctt
    15421 tttttaatgt aaccttcatc atatctgcgt tattttatta tatgtaatct aatatagata
    15481 cattaaataa ctttcctcct gtttgtagtg aagcttgata agttaactct gctgccttta
    15541 attgaacaag tttttcacca taatctattt cttcattttg tgacaaaata tcctttaact
    15601 gtatcaaact atcatcgttt ttttccttca tagcctctac tgtatttgct tttgtcccat
    15661 atatagacct aacatttact gtattttcta ttagttgttc catatcgccc aaacaagttg
    15721 ttgtaagctc ttttatagca tcagcatctg gatttgctaa tttttttgat atgttattta
    15781 actcttcaaa tgggtcagtt tttgttgggt caaatccaag gctactcaat ttaacgttat
    15841 aatctacact tattccttga gctatatcca tactcaaaga gtcatttaat ttattaagat
    15901 ttagactaga attaaacttt aaactcactg aaccatcagc attatctata cattcaacag
    15961 gtggtacccc cgtatttgaa cctgaaaata tgtacttccc ttcatgtgtt gcatttaaag
    16021 catttgcaat ttctttcatt ttttcattag tttcattact aagagtcttt aattcatcat
    16081 ctgagtatgc accatcacca cttgccacca aagttttctt tatgtctgat aataattttc
    16141 caagctgatc taatgcagta tcagtagttt ctaaccaatt ccctgtatct ttacaattct
    16201 caacataagt ctccatagtc tttatttcac tttttgagtt taatatcttt attgctttat
    16261 atggattatc tgatagcttg tttatttctt ttgttgtatt aacttgtcta ttaaccttat
    16321 ccaatctttg tagattatct tgtagtgagt ttagatagct gctagacatc atacctgtac
    16381 tgactctcat tataaattcc cccttctatt aaattattcc atttattaca acatccagta
    16441 gttgcacagc tgttgacata acttttgcac tagcttgata tgaacgttgt atttttataa
    16501 gctctgtcat ttcttcatcc aaagatacac ctgaaacact cattcttgag ttatcaatat
    16561 tatttataat tttacgttga tttgcttcat cttgatttac tttttgagaa tctaaaccga
    16621 ttgattctgc aaaggcttta tagtggtcac ttattgtact tacttttcca ccagccaccc
    16681 caatatctat tttttcatcc tttaacttag caatttttga agcttcatca gcagtaagct
    16741 ttaattcatt tgggttactc tttatagctg gatttatctt tataatatct tcaccatcta
    16801 catttgaagt tataaaaaaa tctgttactg ttattcctgc agacgtatta taaacatcat
    16861 ttatctgttt agctaatcca tcagatacat cttttaaatc gcttttatat gtatcaatct
    16921 taccatgcat ttcaaacaag ccttgtaagg tccctgatat acctgttttc caagttccat
    16981 ctggattttt taattcatta agttctaatt taccatctgc aagcatattt ttaacatcac
    17041 tattttcaaa gtctagttta aaactcaagt tatctaaaat tctatctctt tcatcaagca
    17101 aatcatttgg tgtagagcca gaaccagata taatatcaat agatttattt aaatcttcaa
    17161 gactttttaa catgtcattt atttgtttta tctcagtgtc tatattagaa tctatatctt
    17221 cttttaactt atcaagcttc ttataacttt cacttattaa agttgctaaa ttagatgcgt
    17281 tttctaccac tagacttttt ttactgtcac tatttatatc ctttgataat gactcccatc
    17341 ctttaaaaaa atcatctata cctgcagata ttccatagtc tgatggttcg tttagtatag
    17401 tttctatagt attgtaataa tcatattttg aaactgtctt accatactta tgagcttctg
    17461 ccctaaattg gtaatcataa aaactatttc tagctcttgt tacatccgtt acatgtactc
    17521 cagtccctaa ttgtcctctt gcaattgaat tactcaaact aggcatatac attggactat
    17581 ttgtagcaaa ctcggtcctt tgcctagaat aaccgtctgt atctagatta cttatattat
    17641 gagaaattgt ctgtattgct ttttggtgcg cattcattcc tgaattagct gtatttaaaa
    17701 tggaaaataa tcctgccata tttctcctcc taatatttat tttctaatat ttccataaga
    17761 attataagtt gtagtttgct tatcagctgg ttttatgaaa ttaatcattt ttttcgtaaa
    17821 aataagttct ttctttatta gagaatcatt tgattcttgt tgatttttag ctaaagcaag
    17881 aatgcgtttc aagtctgtat atatttcttt taaatatcca tcattactat tttcaacaac
    17941 ttcactcatt gaaatatcat taccaaaaag ttgtcttctc tttatttcta tggatgcaag
    18001 ctccctgcta atatcttcta gtttaggatt taacatattt aaattttcta agtctttttc
    18061 aactatataa tcaaattgtt cttttaataa ttccatcatc ttactaatta tatttttttc
    18121 ttcatatatt ataactttta agtctgtaat catgacttta tcctcgcata tcctctatca
    18181 tttttttcgc aatttctttt ggttttattt tgtaagttcc attttctaat gcatttttta
    18241 tttcatcgac ttttttattt ctaatattct catcactcat atttgacaca cttgccattt
    18301 cctttgcaaa tttagataac tctatcttgt ctgttttatt aattttttta acctcttcca
    18361 ctttttttat accagatgta ttctttaaat aattattcac tacaatattt gaacttacat
    18421 ttttaatatc catagtttca cctccaaaat tacatactta taatttagtt atcgctcata
    18481 aaataaaaat atttatataa ttcacacata ttttaaattt tttataaaaa tatcccctta
    18541 gctaaactca tacagaatag actaaaggga tatatttaaa atgctaattt acatctgaat
    18601 cttctatttt agataatctt acagataact tctcatttgt aataatgctt tctccataag
    18661 caaacaatct atcgtttgca tatatatcta atttttcatc cacattctta cttaatttta
    18721 ttatagagcc ttcttttaaa tctaatatat tttttattgt ttctgttgta tttcctatag
    18781 aaacagctac ctttactttt gcattaaaaa cattttgatt ttcttctata acaccagatt
    18841 tttcttctat taattgccca aaattcaatt catatatatc ctcttttttg ctattcataa
    18901 aatttttctc cttgtataac cttatctata catatctatc aataagcctt gtatcatgtc
    18961 aacagtagaa acaatatcta tgttttcttt ttcttcacta attaacttac tagtcatatc
    19021 ctcaagttta ctatcaacaa tctttacatt cttataatac tgatgctccc aaaagctact
    19081 atttttatca agtgaataca tattatctac aacagatttt aggtattctt ttactgcatt
    19141 tttatatata ctaatatcgc catagttttg agtaagtaca agcctttctc ctgccttttt
    19201 tacactttcc aaatttcgtt ttatatcttc caatgaagaa gattgtttta aagagtttaa
    19261 tttgtcagaa aatttattac tctcttttag ttttttaacc tccatattta tacttggact
    19321 tatgtttagc ttatctatat ttattcccat aataaagcct accctctata atatttcata
    19381 accttaggaa tataattttg tgtttctttt ggcattttat acaggtcaga gattgattct
    19441 actcctctac ttttcattct tccaggacca ccattatatg ccatcaatcc catttctatg
    19501 ctccctccaa acatatcaat gtattcctta atatgtctag ttccagcatc tatattctgt
    19561 tctatattaa aagggtctgt aacccctaag tctctgcagt tttctggcat aagttgcata
    19621 agacccttag caccagcaga agaaacggta tttggattaa aatctgattc aactttaatt
    19681 attgctttta taagatttga atctacgtta tatttttttg atgcttgatt tacagcattt
    19741 tcaattctca actttacatt tttatctttt gtatcaacat ctataaatct actagtggta
    19801 ttatttacta cattatcaag ttcattaaat aaattctcag aattagacaa taaattgtag
    19861 ctattagaag tatgttgatt attttgtgat aatgctttta acaaggtcat cataatcata
    19921 tcaaatccag atgaattatt atatgtacaa ttacattgat ttgtagtatt actaccagat
    19981 aattgagtca atgctaatat attaattagt gaacttatct cattcaatat aaaacgcccc
    20041 tttctaaatt ttaaagttat actgataaaa cagctttcca ccaagattca ttttctatat
    20101 accacttaac agtttctttt atacccaaat caaagtcata ttttgccttc caacctaact
    20161 cattctctat tttagttgca tcaatagcat atcttaaatc atgaccaggt ctatcattta
    20221 cataggatat taactcttca gatttaccta aaagatttaa tatcattttt actacttcta
    20281 gattacttct ttcattatga cctcctatat tgtaaatctc ccctatattt cctttatgta
    20341 ttattaagtc tatagcagta caatggtcat atacatgtaa ccaatctctt acattttccc
    20401 cattcccata tactggcaat tgtttgtttt ccaaagcatt taaaatcata agaggaatca
    20461 atttttcagg aaaatgatat ggcccataat tgttggaaca cctagatata gttgttaaca
    20521 atccatatgt tctataataa gaacttacca ataaatctgc tgatgcttta gaagctgaat
    20581 atggagaact tggattgata gaagattgtt ctgtaaagaa taaatccttt ctatcaattg
    20641 gtaaatcacc atacacttcg tctgtagata tttgatgata ccttttaact ccatatttta
    20701 tagatgcatc taaaagtact tgtgttccta taatgtttgt tttaataaat aaatcaggat
    20761 tctcaactga cctatctaca tgagattcag cagcaaagtt aacaactata tcaaaccttt
    20821 cttcttcaaa tagcttatac acctgctccc tatttgaaat atctccttta acaaatttat
    20881 aattggaatt gtcttgtatt tctattaatg tctctaaatt accagcataa gtcaacaagt
    20941 ctaggttaac aataaaataa tctacatact tgttaagcat ataatgtata aaattactcc
    21001 caataaatcc tgctcctcca gttatcagta tttttttcat attttttctc ctaaatgtta
    21061 attctaataa atttttatta acgtcttgta caaatatctt taaaactttt ttgttttttg
    21121 tccttttctg ataaaaatat ttcttcaatt ccttttaaag gccactcaat agatatttgc
    21181 ttgtcattcc atataaagcc attctcaaat tctgggtaat aataatttgt acatttgtaa
    21241 ctaaatactg ctttttctga taaaactaaa aatccatgtg caaacccttc tggaatataa
    21301 aattgttttt tattcttatc agacaatatt acaccttccc atttcccaaa tgtttgtgaa
    21361 tctttcctta aatcaactgc aacatcgaaa acttctccct ctgtaactct gactaatttt
    21421 ccttgaggat atttaacctg aaaatgcatt cctcttaaaa cgccttttct tgagctagac
    21481 tcattatctt gaataaaaca catatcaaga ccctgctcta taaaatcctt tttattatag
    21541 ctttccataa aatatcctct ttcatcataa aatacatttg gttcgactat atataatcct
    21601 tctattttag tctttataac attgattttt cccattataa taaactccct taataaaatt
    21661 tataataaat tttatctacc atattacata tcttcactag ccaattttaa tagatattga
    21721 ccatattcag tttttatcag tggcttggct agtttcatta attgttctct acttatatat
    21781 ccttttgtat atgcaatctc ttctaaacat gctatataca gtccctgtct tgtttgaacc
    21841 acttcaacat aattagaagc atccaataga cctttatgtg taccagtatc aagccaagcc
    21901 atacctcttc ctagaagttc cacttttagt tttcctcgtt ttagatattc actgtttata
    21961 gaggttattt ctaattcacc tctatctgac ggttcaacta gctttgctat ctctacaaca
    22021 tcattatcat aaaaatataa tcctggtata gcataattag atttaggaac tgtaggtttt
    22081 tcttctattg acaatacatt aaaatcctta tcaaattcta caactccaaa cgaatttgga
    22141 ttacttacat gatacccaaa tatagttgct ccttttcttt taactgcatt ctccaacctt
    22201 tcactaaatc catatccata aaatatatta tcccctaata caagagacac tctgtcattt
    22261 cctataaatt cttctcctat tataaacgct tctgcaattc catttgcttt ctcctgaatt
    22321 gcatatgtta tgttaagccc tagtgaactt ccatctccaa ataattcttc aaatgctttt
    22381 atatctcttg gtgtagatat tattaatata tcttgtatcc ctgccaacat aagaaccgac
    22441 atagggtaat atatcattgg tttatcgtat attggaagtg tttgttttga tatacatttt
    22501 gttataggat atagtcttgt accagaccct cctgctaaaa taattccttt catctttttc
    22561 catccttaaa tttgtttttg tcatattatg attatcggat aaataaaaga aatatttata
    22621 ttttttataa tatcttattt tcttttctca aaataataca tttacttttc atttatatca
    22681 tatctttcta aatattcttc aatggattct tcccactttc taaaagaatt taaaccaatt
    22741 aacttcaaca taaaattatc caatacagaa tatagtggtc ttggagcctt acatttaaat
    22801 tcgttactct taattggatt tacttttata tctatatttt ttagtttaaa tatctttttg
    22861 gcaaaatcat accaactaca ctcaccttca catgtagcat gataaactcc ataatgttct
    22921 gtttccatga tttttattat aatttttgct aagtctactg tacttgttgg agttccaaac
    22981 tggtcattaa ccacattaac ctctttattt tctaaagaaa gttttatcat ggtctttaca
    23041 aaattattcc cattaccata taaccatgct gttctcaata taaagtactt atgtgagaat
    23101 tgttcaacaa atttttctcc cataagttta ctttttccat atacagaatt gggttcagtt
    23161 ttattgtctt ccctatatgg atatttagaa aaaccattga atacatagtc agttgatatg
    23221 tgaataagct tagcattaac tttttctgat gctattgcta gatttctcac tcctaaagca
    23281 tttattttgt atgcaatttc gatattatct tcacatgcat caacctttgt atatgctgca
    23341 cagtttacaa ctacatcagg tttattatgc aaaataaaat tatctacatt agtctgattg
    23401 gatatgtcca aatcacttct tgttgtagct aatataacat attttggttg gtttatactt
    23461 tgattgattg cttctaattg gttaaccaat tcttttccta attgaccatt agaccctgtt
    23521 attaatattt tcattaatta tgttgtcctc caataaatat tcaattttac ttaataatta
    23581 attataacac agcttgtatt ttaacttagg ttgaacttat tatttcttag ataaataaaa
    23641 aagagatgta atattaaatg attatcatca tattattaca tctctttaaa tctaaatatc
    23701 taaattatat attctagcta tcaattaaac atcaaagctt tatctaaaat atttaactcc
    23761 agattcaaat atcttttggt ctttttcacc tataatattc ttatatactt cctcaccaat
    23821 tctttcagaa tgtcccattt taccaaacac tctaccatca gcactagtta taccttcaac
    23881 agcatagaat gaaccatttg gattaaactc aatatcataa gtagcttcat tattaaagtc
    23941 aacatattga gttgctactt gtccattagc aattaattct ctcataacat cttcactggc
    24001 aacaaactta ccttctccat gagaaattgc tatattatga atatctccaa cattagtttg
    24061 agctaaccaa ggagatttat tagatgaaat tctagttcta gcaatcttag cttgatggcg
    24121 accaatatta ttgtaagtaa gtgttggagc actttcactt ggaactctta tttctccata
    24181 tggaactaac cctaatttaa ttaatacttg gaatccatta cagataccta acattagtcc
    24241 atctttttga gttaaaaatt catttatagc ttcttgcact ctaggatttc taaatacagt
    24301 agctataaat ttaccagaac catctggctc atcaccagca ctaaatccac ctggtaacat
    24361 tattatttga gaagatttta tttcgtttac tattgtatct atagaatctt ctatgtcttt
    24421 ataagttaaa ttcttaaata ctcttatact tgcattagct cctgctcttt caaatgctct
    24481 tgcagagtca tattcacagt ttgttccagg gaatgctggt ataaatactc ttggagtagc
    24541 tatacttaat gatgattttt tagcttctcc ttgtgatata taacttatag tttctatctt
    24601 tgattttact tcttctgttt tagttgggaa tataggctct aaagtactac aatgtgcttt
    24661 atataattca tctaatgcaa gttcttcacc tttgataaat atactagttt ctttaactgt
    24721 acttccaagc acaacataat tgtatccttc taacatactt aaatcatcat ttgctaattc
    24781 aagtactata tttccataac tagcctcaaa taatttatca tctgtaaatg ctttttcagc
    24841 ctcttcacta aacttaaatc ctattttatt accaaaagcc attttactta ttgcttcacc
    24901 tacacctgca aatcctaatg catatgttga taaaacttta tttccatgta taagttctct
    24961 tactttatct aagtttctct ttaattcctc aaaatctaca acatcatttt ctgccttgtt
    25021 tacacataac attactactt ttgagtctgc ttttttaaat tctggagata ctactttctt
    25081 cgcatctact gtgtcaacag caaatgaaac taatgttggt ggaacttcaa tatctttaaa
    25141 tgtaccagac atactgtctt ttccacctat agcaggtatc tcaaatttac tttgtgcata
    25201 aaatgctcct aaaagtgctg agaatggttt accccatttt tctggattgt taccaagttt
    25261 ttcaaaatac tcttggaatg taagtcttgt tgtactataa tttcctccaa tagcaaccaa
    25321 tttacacaca gattctacta ctgcatacaa ggcaccatgg aatgggctcc attttcctac
    25381 ctttggattg tatccataag tcattattgt agaagtatta gtttctcctc caagtactgg
    25441 tatcttagca accatacctt gtgttggagt cgcttgatac tttcctccaa atggcataag
    25501 tactgtatta ccaccaattg tattatcaaa catctctact aatccttttt gagaacaaac
    25561 attaaggtca gatagtactt ttgtaaactt gtctttcata cttgcacact taacatcttt
    25621 tactatttca ttagagctaa agaatgtatt ttcttcatct actttagtaa catgaacatc
    25681 agtagtttgt tttactccgt ttgtatctaa aaactctcta tttatatcta caattgcttt
    25741 tccattccag aacattctta aatatcctgt atctgttact gtagctacat gcgtagcttc
    25801 taagttttct tctactgcta attgtataaa tttatcttta ttttcttttt ttatggctac
    25861 tgccatacgc tcttgtgact ctgaaattgc aagctcagtt ccatcaagtc catcatattt
    25921 tttaggaact aagtctaagt ttatatctaa gctatcagca atctctccta tagcaacaca
    25981 aactccacct gctccaaaat cattacatct ttttatcatt tgagcaactt ctttatttct
    26041 aaagaatctt tgtatttttc tttcgttagg agcatctcct ttttgaactt ctgcactaca
    26101 agttaagatt gactcttcac tatgttcttt agatgaacca gttgctcctc cacagccatc
    26161 tcttcctgtt tttcctccaa gaagaacgat tacatctcct gcttcaggtc tttctcttac
    26221 tacattctct tttggtgctg ctgctataac tgcacctatt tccattctct ttgcaacaaa
    26281 gttttcatca tatacttctg caacttgtcc agttgtaaga cctatttggt taccatatga
    26341 actatatcca tgagcagctt ctgtagttat ttttctttgc ataagttttc caggtaaagt
    26401 atcttctaaa gtcgttcttg ggtcagcact tcctgtaaca cgcattgctt gataaacata
    26461 acttcttcct gataatgggt ctcttatagc tcctcctaaa caagtagctg ctccaccaaa
    26521 tggttctatt tcagttggat ggttgtgagt ttcattttta aacattacta gatatttttc
    26581 catcttacca tcaacttcaa catctacgtt tatactacat gcatttattt cttcactttc
    26641 atctaggtca tttaatttcc catttttctt taattctttt actgcaacag tggctatatc
    26701 cattaaacat atatctttat gtctgttcac atatacattg tctcttgagt taagatacat
    26761 ttgatacgct tcttttacta tatcattaaa cttaccatct tctatcttta cattttctat
    26821 ttcagtcata aaagtagtat gtctacaatg atcagaccaa taagtatcta atacttttat
    26881 ttctgttata gttggatttc tcttttctgt atctttaaag tatttttgaa cgtgttgtaa
    26941 gtctcctaaa gtcattgcta atcctttttc acttacaaat gtttttaatc cattttcatc
    27001 taaatctata aatccatcta atacttcaac tgttgttggt atttcagttt ccatttttaa
    27061 cgtttctggt ttttctaaag atgcttctct actatctact ggatttatac agtaatcttt
    27121 tattttagaa aactcttcat cagttatttt gcctgataat atataaactt ttgctgtatt
    27181 aatagcaggt cttatccctt gatttacaat ttgtacacat tgagctgccc aatcacctct
    27241 ttggtcgtat tgaccaggaa gaaattctat tgcaaatact ctttcatcat ttaattttgg
    27301 tatttcttca taatatacta catctaaatt tggttcagag aatattgtct ttgctgcatt
    27361 ttcataaact tcttctgata ttccttctac atcatatcta tttaatattc ttaaattttc
    27421 tatattatct atgtgtaaac tttctactaa gtcttttttt aaagctttag cctcaaggtc
    27481 aaaaccttct cttttttcaa ctaatactct tctaaccatt gagtctttat tttcagtatt
    27541 caacatacct tatcctccat tttagaaaaa ttatcttcct atgtctgttc tatagtgcat
    27601 tccttcaaag tttatcttct ttatgttttc ataaactttt tctgctgcat ccttaactgt
    27661 agttgacttt gcagttatac caattactct tcctccattg gtaaccaaat taccatcaaa
    27721 catcttagtg ccactatgga atactactat atcatcatcc aagttttcaa gaccagttat
    27781 aattttccct ttttcataac tgtctggata ccctcctgat gtaagcataa cacatacagc
    27841 ttcatcatca ctatagttta tttctatatc ttttaactta ttatctagta tagcttccat
    27901 tatctcatgt aaatctgttt ctaatctaaa taaaacagat tgtgtctcag ggtctccaaa
    27961 tcttacatta tattcaagta ctttttctcc atcttcagtt atcataagac caacaaataa
    28021 aataccttta aaattaagtc catctttttt gaagccttct aaagtttttt ctaatactgt
    28081 ctcttttact ttatttgcta attcttctgt atatatttca ctaggtgaaa aagttcccat
    28141 tcccccagtg tttggacctt gctcatagtt gtttactttt ttatggtctt ttgcacttgc
    28201 cataggaact attgtatcat tatctacaaa tgctagaatt gaagtttcta ttccttttaa
    28261 aaactcttca attactattt tatctccagc agctccaaac ttcttatctg acatcatttc
    28321 ttttaaagtt gctatagcat cttctctatt ttcaggtatt acaacacctt ttccagcagc
    28381 taatccgtcc gcttttatta ctactggata tccaaaacta tctatttctg atatagcttc
    28441 ttctaagttt gtatattctt tatattttgc agtaggtatg ttatgtttta tcataaattc
    28501 ttttgaaaaa gctttacttc cttcaagttg agcacatttt ttattaggtc caaatactct
    28561 taatccttct ttttcaaact catctactat tcccataact agtggtactt ctggtcctac
    28621 tattgtcaga cctatttcat tttctttggc aaattttaaa agttcttcta tattagtatc
    28681 acctatattt acacattctg ctacgtttgc aattcctgca ttacctggtg cacaataaat
    28741 tttttctaca tttttttctt tgcttagttt ccagcatata gcatgctctc ttccgccacc
    28801 gcctacaacc aatatcttca taatatcctc cttagtgttt gaagtgtctt attcctgtaa
    28861 ataccataac catatcatac ttgtcacaag cttctattga atctttatct tttatagagc
    28921 cacctggttg aactactgct gatattccat attcatgagc taatgttaca caatcatcaa
    28981 atgggaaaaa tgcgtctgac gctaaaacag ctcctgtaaa atctttatct ttgttatttt
    29041 caagagcatt ttttaatgcc caaattcttg atgtttgtcc acaacctaaa gctagagttt
    29101 gtccattttt tactattgca attgcatttg atttcatatt ttttactact ctcataccaa
    29161 attccatgtc cttaagttgt gcatctgttg gtttttcctt tgttacattt tcactttttt
    29221 ctgctaatat attgtttcta tcttgtatca ataattttcc atctaaatat ttcatttcat
    29281 atggttgtaa actattttct attttagcta attttaatat tcttagattt ttcttttgtt
    29341 taagtatctc taaagcttct ggttcaaaat catatgctac tactatttct aagaatattt
    29401 catttaattt ctctgctgta gctttatcta tagtagaagt tattcctact attcctccaa
    29461 atattgaaac cttatccgct tcataacatt ttgtatatgc ttcaaaagca tcttttccta
    29521 aacctacccc acatgaatta gcatgtttta tagctactga aactacttct tcactatctt
    29581 taaattctct cattacttct aaacatccat gtaaatcatt taagttgtta aaagaaagtt
    29641 ctttaccgcc taattgctca taatttaaga ttggattttt tgcatttgac tgtgaatata
    29701 agaaaccatt ttgatgtgga ttttctccat atcttaaagt ttgttctttt tggaaagtta
    29761 aatttaatat gtcaggatac gtatctccaa cttctccagc aaaataacta gatattaacg
    29821 catcatatct tcctgtagtt gagaaagctt tatatgataa tttttttctg tcttctaatg
    29881 tcattgtatc tgtttttaat ttttctatta tagaatcata atcttttata tctactacta
    29941 ctattacatc cttatagttt ttggcagcag aacgtatcat agatggtcca ccaatatcta
    30001 tattttcaat tatttcatca tgactttttc cagcttttaa agttccttca aaatcatata
    30061 gatttacagc tactaaatct attgaatgta ttttatgttc atttacagtt tctacatgag
    30121 attctttatc tcttttataa agtataccac catgtatata tggatttaat gtttttactc
    30181 ttccatctaa tatttctgga aaatttgtaa catcttcaac ttgcattaca ttaacaccat
    30241 tttcttttaa tgttttaaat gtatttcctg tagatataat ttcataatct aatttattta
    30301 actctttagc aaattccact acaccagtct tgtcagttac acttattaaa gctctcttac
    30361 tcatttataa acactctcct accttgaagt tttaatttat tttcacaaaa aagacttata
    30421 ctttctttta aaatcctatg ttcaacttct aacactcttt ttgcaagtgt ctttgcatca
    30481 tcatcttgat ttaccttaac tacatcttgc attatgattg gtccagtatc agcaccttca
    30541 tcaacaaaat gaactgtagc accagttact ttagctccgt aatctataac accttgatga
    30601 actttttctc catagaatcc agctccacaa aatgaaggaa ttaatgatgg atgtatgttt
    30661 atcattttgt ttctaaactc attaaccagc tttgggctga ttatttttaa ataaccagcc
    30721 aaaactacta aatctatttt attttcctta agaatttcta ttatcttgtc ttcatcagtt
    30781 tcacaaattg cttttatgtt atgattttta gctctctcta gcccataagc accttgctta
    30841 cttgagataa ctactttaac ctgacctttt atttctccag actcagtacc atctataact
    30901 gcttgtaaat ttgttccccc accagatatt aaaactccta tatttagcat aattctacac
    30961 cttcatgact atctactact tcaccaatta tataagcttt atcttctaat aattcactat
    31021 attttttatc tacataatca gcattgtcat tttctctatt atttataaag tttacaacat
    31081 catcagcatt ttctttatct actattaaaa ctagcccaat acccatgtta aaactcttat
    31141 gaagttctct ctcatcaaca gcattaaaac cttctatcat tttaaatata ggaggcttct
    31201 cccaagattt tttattgata tctagtccta atcctttagg tataactctt gttatatttt
    31261 ctataagccc tccaccagtt atgtgagcta cagcttttat gtcatgttta gctaatacat
    31321 ctaaagctaa tttaacatat attttagttg gtgttaaaag tgcttctcca actgtcattc
    31381 ctagttcttc aacatatcgt tccattttat aattgtaagt ttctaaaaat atctttctta
    31441 taaaagaaaa tccattgctg tgtactccac ttgaagatat accaaccaat acatctcctg
    31501 atttaacatt gtttccagaa acaatctttt ctttgtctgc tattcccaca gaaaatcctg
    31561 ctaaatcata gtcatcttct ccatacattc caggcatttc agcagtttct ccaccaatta
    31621 gtgcacaacc agacattttg caaccatctg caattccacc aactattttt tctatatgct
    31681 caggcactaa tttcccaaga gctatatagt caaggaagaa taatggtttt gctccttgac
    31741 aaattaaatc attcacgcac attgcaacta aatctattcc aacagtatca tgcttgtcca
    31801 tcatctgagc tatttttaac ttagtcccaa caccatctgt agatgcaagc aaaactggtt
    31861 ctttcatacc tacaaaatct tttaaactgt ataatccact gaagttacct aaatctccaa
    31921 ctacatttcc atcataagtt ccttttattt taccttttat aaggtctact gctctatttc
    31981 cttcatctat atctacacct gattctttat atgttaacat gaaagtagtt cctccttatc
    32041 aattttctta actgggtatt ctccatcaaa acaagcttta cagaaagtag acttatgttc
    32101 tgttgcatct aacattcctt ctatatctag gaattttaaa ctatcacatc caatatattc
    32161 tctcatttct tctacgttgt tacttgatgc aattaattta gagcgatttg gagtatctat
    32221 tccatagtaa caagagtaag ctactggagg agaagttatt ctaagatgaa tttcttttgc
    32281 acctgcttct cttaaagatt ttactaattg tttagatgta gttcctctta caattgagtc
    32341 gtctactaaa attatagatt ttccttttat tattgttgaa agaggattta attttatttt
    32401 tactgctatt tctctttctt cttgtgtagg ttttataaat gttcttccta catatctatt
    32461 tttaactaag ccttcactta tttttaatcc actagcattt gcataaccaa tagctcctgg
    32521 ccaacctgaa tctggaactg gaactaccat atctgccttt acagtttcat tctgagctaa
    32581 tctctcacca catttaattc taaattcata tgcatttaca ttgtctatag ttgcatcatt
    32641 tcttgcaaag tatatgtgtt caaatataca acttttctta actggtttat aattttctga
    32701 ataaaaatat gattttagtt ctccatcttt gactactatt atctctcctg gctctacatc
    32761 tcttataact tctccgccaa gtatatctat tgcacaattt tctgatgcaa atatatattc
    32821 atcacccttt ttaccaagta caagaggtct gaatccatgt gggtctctaa ctgcaaccaa
    32881 ttcttcttga ctcattatta caagagcata tgcacctttt atataatcca ttgtcacttt
    32941 taaactttct actatatccc ctttgtaata tctagcaagt atatataaaa tcacttctgt
    33001 atctgaatta gtttggaaca tatatccatc ttcttctagc atatctttta aataatttgc
    33061 attaactaga tttccattat gagctatggc taattgtctt tttctacaac ttccaactaa
    33121 aggttgacaa ttagaaacat gacttcctcc agctgttgaa tatctaacat gtccaatccc
    33181 tatatttcct tttaacttag ataattcatt ttccttaaaa acatctccaa ctaatcccat
    33241 atcttttttg tacttaattt ctccatcatc taaaagagct aaaccacagc tttcttgccc
    33301 cctgtgttgc atagaatata aagaataata tagttcttta gttacatctt tattagagta
    33361 aatccctaaa actccacaca tacatttgcc tcctatttat tattcattct tgaaagtact
    33421 tccgtataac cttctacaag gtctccaagg tcttttctaa atctgtcctt atctaacttt
    33481 tcatttgtat ttacatccca aagtctacaa gtgtctggtg atacttcatc tgccaatatt
    33541 atatttcctt cagaatcctt accaaattct attttaaagt caactaactt taggtttaat
    33601 tttaagaaaa atgcttttag taactcattt atttttaaag tttcttctct taagaatttt
    33661 aactcttctc tagttgctaa cttcattgcc actgcataat catcatttag cattgggtca
    33721 ccataagcat catttttgta gcttatttca aaaattggct catcgaaaac tacaccttct
    33781 tcaagaccaa ctcttttaca gatagaacca gcagcaatat ttcttactat tacttctaat
    33841 ggtaaaatct ctactttttt aactaacatt tctctatctg ataaactttt tataaagtga
    33901 gtatttatat tattttcttt taacatttca aagattattg ttgatatctt attatttagt
    33961 ataccttttg aagatatttc tgcttttttt tctccattaa atgctgttgc atcatcttta
    34021 taataaacaa catattcatt ttcattatct gtagaatata cttgttttgc ttttccttca
    34081 tataataaca tttctgctac ccccttaaat tattgtcgtc ttctaacacc ttttgtgcca
    34141 tctcttctct atatgatttc aaatcctctt ttaattttgg gtattttaaa gtcattattt
    34201 gtaatgccaa taaagctgcg tttaaaccta agtctattgt aactgttgca actgggattc
    34261 cttttggcat ttgaactatt gataaaagtg agtctagtcc atccatagtt gatgatttaa
    34321 taggtaatcc tattacagga attaatgttt gtgaagctat tacacctggt aagtgtgctg
    34381 cttttcctgc tgctgctatt attacatcag tatcatcttc tatttctttt aggaatattg
    34441 aaagttgttc tggtgttcta tgagctgata atgctcttgc tacaacttct attccatatt
    34501 tttcaagcag ttttatccct tcttctaatt ttggataatc tgattttgaa cccataacta
    34561 ctgctacttt cattagactt cccctctccg tttataagtt attttacact acaaatcatt
    34621 cgtattatta tacgaattat acaccttaaa ttttatatta atatacatat attatcaact
    34681 cttttttgaa attttttaaa aaaaatattt cttgtaattt tttcattcgc tacgtatcga
    34741 actttattta tgtttttttg ttaaaataaa aaacagatta tctcaaaatt ggttttctaa
    34801 ttaaatttta agcccaatac tcttttttaa atcttgtata ttatttttta ttacttcttt
    34861 tttaattaat actttggatt tatagtcttg ttttttagtt ctgaatgtta cttttactta
    34921 gataaaacca acacaaagtt tattctttat ataagtaata gctaaaattt aaagtaatga
    34981 tattatataa atagaaataa ttttatttaa aggagaatct gtatgttaga agttataaaa
    35041 aatagacgtt caataagaac atatatagat aaaaatatag aagaagataa aataactgaa
    35101 atactaaaat cagctatgca agcaccttca tcaaaaaatg ctcaaccttg ggaatttatt
    35161 atagtagatg ataaggaact gctgaaacag ctctcaaagt cacagcatag agcaaagcat
    35221 atagaatttg caccattatg tatagtagtg cttggaaata gagataaatt cttaaaacct
    35281 ggtaaatgga tacaagattt aggtgcttgc acacaaaatt tattattaga agttactaat
    35341 caaggtctag ctgcttgttg ggcaggagta tttcctaaaa ataaagtggt aaataaagtt
    35401 agacaaactt tagacttacc attaaaatta gttccttatg cacttatatc tattggatat
    35461 agtgaagaaa aaaatgagtt tatagaccga tttgatgaaa ataaaataca tagaaatgta
    35521 tataaaaata gataatgttt agtaatataa atgaaaatac atagtaaaaa tgcctttaga
    35581 atacatttgt attctaaagg cattagcttt gactaaacac ctaattagcc tctaaggtaa
    35641 aatttaacta tcatttatta tttatatacc agttgaatat tatatccttc aatattttta
    35701 tttatttcaa atatattaag aatatcctac aattaaagtt agctattttt cattaaagta
    35761 ttttattgac acactcccca tagctaaagc aaggggattc ttggtggcta acacacttat
    35821 ttatacttaa acagtacaac agaggtggtt acaaagccaa gctacttcgg acggtgtctt
    35881 cgccactact accttaaagt tattcgccca cacatggctc aaacggtaga tacttttaaa
    35941 tttattggtt gcttatgatt tattctaaaa tctactaaac catatttatg ctttttattt
    36001 aaaatattcc aagcaccaac tatatctcta tgagtttcat aaccacaagt acaactataa
    36061 gttctaccag ttggtttgtg ttttttagaa cactttgggc aagtttgaga agtgtagttt
    36121 tctttaacta acttaacttc aataccttgc tcttttgctt tatattttat atagtccttt
    36181 attcgaccat actccatttg agatattttc tgtctattaa ctctgtttaa tcttttcttt
    36241 ttcttagtat ctttttcaac cccttttata tccccaagaa ctatttcaca taccttctgc
    36301 tctacacaaa agtctataaa ctttctagtt gtttgatgat ataaattcat taactgatta
    36361 tctgttttac ttcttatctt attttttaat ctccataact tctttgattg tctactacct
    36421 tttttacatt tacttaatct tttagatata taagcataca tcttatttct aaattgttta
    36481 atacttctgc cttctctatt agatagaata agccctacac ctttatttgt tacactagct
    36541 atcgaatgta tttcccctaa gtctacacct acggtattag tgcctttggc tacaactcca
    36601 tttaattcat tgtcaaaagt gtagtataaa taatatccat ttcgccatac tatttcagca
    36661 gtttttatat tctcaacatt aaaatctaat tctaaatacc tgccttttga cattgataat
    36721 tttaagttgc ctttagcatt tctattaata gcactagctt taaaaggtat tatataaaat
    36781 ttcttagtct tgtatggata ttttgcttta gtattacctt tttttcttaa ttctgatata
    36841 gtctttctat ttgcatcaaa tttatctgat atagcttgaa tagtttgact atgaagattg
    36901 tacttacctt tgattaactt ttgtatatca ttctttttaa tccattgttt atgttcaaaa
    36961 tagtattctt tagataagtt aactatgtag ttccaacagt tagcactttc catttgcttt
    37021 tcccttacta attcaaattg tgttctagtt aggtttaatt tcactctatg agtttttatc
    37081 atttctttgt cctaacttgt tgattttcta tatattgttt aactacttct aaactcacag
    37141 aaccaacagt acctacaaaa tagctattag tccacatgct aggcatacta gactttaaat
    37201 gtggaaagtt ctctcttaat actctcgaac taaagccttt taaacctttt actactttat
    37261 gaattccata ttgagggtca caagatatta ataagtgcac atgttcttta tcagtttcca
    37321 tttctattat ttctgctcca agttcatctg ctttatatgg tagtatttct tttaacatct
    37381 tttcaacttc gccaactaaa acaggtttac gatacttagg gcaaaataca atatgatatt
    37441 tgcaatcata aactatattt ttgttagatt tatacataaa aactaacacc tcctaaatat
    37501 agggtatcag tagcggtatt agattacaag cattattcta acttttttta tattaagacc
    37561 ttacctttca tccctacacc taaaggagag ggcttttcgg caattttatt gtaattatat
    37621 atcatttata taaacaagta catattttat tttgaaacta caaaattatt gtactatatt
    37681 gttttaagtt gttttataat agattatatc atcttattat aggagaaaaa atatgatttg
    37741 tactattaaa ccattaaaga tttcaataaa aaagtctact gaacctctaa ttgaatttat
    37801 atctgatact acctataaaa ataagagttc ttcatatatt aaagaagtct actttacaag
    37861 atatataggt gttgatgatt caattgataa atacatagaa aaaattaaaa atatagatac
    37921 ttattttttt aataatccta caattcccta tattagatta aacaatctaa aaataaattt
    37981 tgacaaagaa caaacagata agatgcttaa aatttttaat atttataata gttctaagtt
    38041 taacagttgg gatttatata atatcgactt tccatgtaaa atagaaaatg acaccttaaa
    38101 ttggactaaa aagattgcat ttaaaaatac tttagattta ttttccacaa gtatgccaaa
    38161 ttgtacatcc tctatcatca aaaactttgc agttaaactt ttgtgttgga tagactattt
    38221 tctacctaaa ttattttata acaaggtgaa gactactatt tcaccaaaga tagtatactt
    38281 tggaaatatt aaaaggcaag aacttttttt cttatatttt ttatcacaac ttgggtgtga
    38341 tattttatat ataaatccaa ataaagatat tcttgattta tatcctgaat gttcaaaatt
    38401 ttctactcta tttgaacttt ctaggaagac tccaaatata ttagaaatac cttttgaaag
    38461 gcatattaca aatataaatt acagtgaaaa taccaataag acttctccta ctacgaataa
    38521 acctaactct aaaaatacac ttaataacac aaatctaaca tctaaaaaaa ttgtacaaga
    38581 tgaggaagta gaactttctt atgaagacct tgcaaagtta tcagcttctg ttgttatgat
    38641 tgttgtgtgc aataacgaaa ataaaccgtt taagagcggt tctggagtag taataaataa
    38701 tgaaggttat atacttacga atcttcatgt agtcaatgat gggtattcat ttttagttag
    38761 atttgaaaat gatgataaag tttatacatc ttaccaaatt ataaaatacc attcagatta
    38821 tgatttagct gtgataaagg ttgatagaaa atgcaagcca atacctgtta aagtcagtaa
    38881 aaaacctgtg agaggtcaaa aaatagttgc aattggaagc cctctaggtc tttttaatac
    38941 agtttcagat ggaattattt cagcttttcg tgattttgaa acggttcaaa tgatacaatt
    39001 tacagctcca atctctagtg gtagttcagg aggagctctt ttagatatgt ttggaaactt
    39061 actaggattg atttccgctg gatatgatga tggacaaaac ttaaatttag ctgttgaaag
    39121 tagtcttgtc aaaatatttg ccaataattt tattgagata gtaaactaat caatcaaaat
    39181 tctacttatc ctattccata ataatcaaga gggtgaaaac attgcatgct tttgccctct
    39241 gattaatatt gaaaaaagta ttaatactcg acatttttat gctcaataaa taattgatgc
    39301 catagacaaa atgtaaatat agtccatatt tttctcgaat tatctctatg acctttaaga
    39361 tgcgtatcta aaagttttat tgcatatttt ttatctataa attcatctac atttgaatta
    39421 taaatagttt cctttacaat atcccctaga gaatctttta accaaactct tattggagtt
    39481 ggaaatccta attttttcct ttgaacaaca tgttctggga ctatttctct aaacgcctct
    39541 ctaagtaaaa cttttgtatt attcttattt attttttgct ccattcttaa attttttgcg
    39601 acatcaaata catctttata taaaaatggt actctaagtt caacctgctc agctgtagaa
    39661 agtttgctaa ttttttgaag tatatcgcct tgaagccatg tattcacatc tatatgttgc
    39721 attttactta tataatcgta attatttttt tgtgcatctc tatacaaatt agacaacagg
    39781 tattcatata tattcttttc cttatactta aaaaagaatc tttttacttc gctgttttca
    39841 aaaacttttg catttcctat atatcttttt tctaatggtg ttgtagctct ataaagataa
    39901 tttcttcctt ttatgtttgg cataagttct gaaactcttc ctaatatact tttaatataa
    39961 gtaggtgaat tgacaactga tttcatagta ctatactctt tatatatatt atatcctcca
    40021 aataattcat ctgctccttc tccagacaat gctactttaa catgtttacc tgcctcttta
    40081 attaagaaat aaagacctac ttctgaaggg tctgctatag ggtcatccaa agaataaatt
    40141 acattcggta atgattttat atattcatct tgtgttatat taatctggat attttctata
    40201 cctaacttat cagctgtttt ctttgctact tctaattcac tatatccttt gacactaaag
    40261 cctgcagaaa aagattttat atttggatta atttgtgaag ccacagtagt tataatagat
    40321 gaatctattc caccagacaa aaatgttcca atttctttct cctcttccat atgagttact
    40381 attgaatcta ctactatatt tcttatatca tcctcattca catctctatt gcctaagtga
    40441 tttttagtat aaaaaaattt tgtactactt ctaaactcaa gtttattata tctctttaaa
    40501 gatagtatac cattttctac cctaaaatag tgacctgctg gtatcagcct tattccttgt
    40561 atcatagtat cttcaggaag tacatattga aatgaaaaat aactttgtaa gcttctttca
    40621 tttatattta aatttttcat atattcaagt aaaaacttat actctgagga aaaaattatt
    40681 gcattttcct tatttatata ataaagtggt tttatcccaa ataaatctct tgctccaaat
    40741 atactttcat tttctctatc atagattata aagttgaaca ttcctttaat cttatgtaca
    40801 caattcttac caaattctat ataagatgct aataagactt caatatcatc atttgtagta
    40861 aaattgtgtc cttttttaat taattcttct tttatttcat gatagttata tatatttcca
    40921 ttaaaaacaa taacttttcc atctttttca aatggtttat ttccataaat agcatcaatt
    40981 acattaaatc tttcaaaatc aaaagttttt tcctcatcct tatactctat tctactagaa
    41041 agtcttgtat tatttacaga ttcttttatt ttaaatttaa tatcttcttt tttagatata
    41101 tcacctttat aataaattga aatattgccg tccatataat caaaattcct ttctgtcctt
    41161 tagtcttatt ctatatttca tagttttgtc tttggcttta aaacttattc aatttaaaat
    41221 gaaattgcta ttaaattctt ataaattatc tgttaatatt tcatattttt tttatttcta
    41281 gaaatactat attaggaggt gatttttatg aagaaaaaaa tattaatacc agttattatg
    41341 tctttattta taatctcaca gtgcataact tcatttgctt ttacacctga aaataacaaa
    41401 tttaaggtta aaccattacc ttatgcatat gatgcacttg aaccttatat agataaagaa
    41461 acaatgaaac tgcatcatga taagcattat caagcttatg ttgataaatt aaatgctgct
    41521 cttgaaaaat atcctgagct ttataattat tctttatgtg aattattgca aaatttagat
    41581 tctttaccta aagatattgc tacaactgta agaaataatg caggtggagc ttataatcat
    41641 aaattctttt ttgatataat gacgccagaa aaaaccatac cttctgaatc tttaaaagaa
    41701 gctattgata gagactttgg ttcttttgaa aaatttaagc aagagttcca aaaatctgct
    41761 ttagatgtct ttggttctgg ttgggcttgg cttgtagcta ctaaagatgg caaattatct
    41821 attatgacta ctccaaatca ggatagccct gtaagtaaaa acctaactcc tataatagga
    41881 cttgatgttt gggagcatgc ttactattta aaatatcaaa atagaagaaa tgaatacatt
    41941 gacaactggt ttaatgtagt aaattggaat ggtgctttag aaaattacaa aaatttaaaa
    42001 tctcaagatt aattaaatat taaaatcctt ttgaaaaaaa cttatgtttg ctatataata
    42061 taataaaaga tagtctaaat ttaccaatat aatgtttata gtgacgattc aggaggattt
    42121 ttatatgata aaagaaacac atgaaagggg aggttatata ttcgcacttt tgatactccc
    42181 ctttataaac aatctatacc tagttaaata cgatattgca tataaaatag tattaattat
    42241 aatttatatt tattttgcat atttaggttc tttatttcca gatatagata tgagaggttc
    42301 ttacataagt aaaaaattta tgttaatata caaattattt ggaagtaggt ttagacatag
    42361 agggtttact cacagtctaa ttgccttatt acttatttat agttttttta aatctctgac
    42421 aatcttcact aacaataata tagttttctc atgtttgtcc agtgggttta taataggata
    42481 tctatctcac ttgtgtcttg accttattac taaagaaggt attgaattgt tttatcccat
    42541 taccataaac atttctttac tacctataaa aactaactca aaaacagaaa aattcatatc
    42601 taagctatta aatttcatag ttattttttt gattggatat caattttata ttttattata
    42661 gttgtagaaa aaataaataa caatattttg aactaagtat aaataagttt tatatatttt
    42721 agtttacaaa agaactaaaa attttgtacc ttaatacaaa aaataaaacg tatcttaaaa
    42781 taatttagta ctaaatcatt ttaagatacg ttttatttcg tacaagctaa atcattttat
    42841 catctttatc attgtgatgt tttttatgat ggaaatcata attatgattt tcaagatttt
    42901 tatttactct atttccaatc ttccatgaca aaaacaccaa taatataaat gatgctatct
    42961 ttaaaggact tgttataaaa ccttctatat cacttgccac gtaacttata actataaaca
    43021 ttacaaattt tcctgccaac attgctggca caaaatcttt taaatctttt ttaccaaaag
    43081 ctgatgcgat agtaactaaa caaccgggca taaatggaca ggcatacgct ataaataata
    43141 acttaaatcc ctgtttatcc atccaactta ttgctttttc agttttacta tttctcaact
    43201 tattaaaaag cttactatca ttaaatctgc ttattaataa gaataatgaa gttgtcccaa
    43261 gtcctgagcc aatccatgat aacaaacatc caataaataa tccttgtatt gcagcatttg
    43321 ctgtaactat agcaacaagt ggtaaggctg gtataaagct ttctataaat gcacttaata
    43381 agccgattat tgtggctaac acccaataat tttgagtcaa atcaagtagt tcttgtatat
    43441 actgcatatt gcttttcccc ttttttaact ttaactatta ttatatactc tatatgttta
    43501 ttatatacca atctcaaaaa atgaatctaa tattaaaaag taaataatct atataagttt
    43561 acagttaagg tataattatt ttattatata ttataacaaa aaaggtgatg tttcaaaata
    43621 aaaatctgaa acatcacctt tttataactt aaattgtcat gtaagttata aaaaatatat
    43681 attagacata atattactaa aaatgtctag tcagagtaaa ataaattaca cagtacaaca
    43741 aatattacta tttaattgag ttacttcttt ataacattta accttcttcc tactctgctc
    43801 aataattcat tcgatatact acatgagtta tttgcaactt cagaagcaga taattcagat
    43861 aaattatctc ttcctattat tattgccgta ttaccaactt ctacattagg tatatctgta
    43921 atatcaacag caagttggtc catgcaaatt cgacccacta ttgctgctct acaaccattt
    43981 attattacgt aactttttcc acaggataaa tttctaggta taccatcggc atagccaata
    44041 gataatatag caattacgct atctctgctt gcaataaaga ctcttccata tccaaaacta
    44101 tcatcttttt gtatttttct aataagaatt acttgtgatt ttaaggataa aacaggtcgt
    44161 aaatcaagtt gcaatttagt cgtatcattt ggagaactta atatcccgta taatgcaatt
    44221 ccaattctta cataattaca cttaagattg ggataattaa aaaaaccata actactttga
    44281 atatgtactt taggaatttt tatacctttt tttaacagaa aatcaattaa cttataaaaa
    44341 cattcaattt gtttatttgt aaagtctaca tcttcatctt tcagactatc tgaaacacac
    44401 aagtgtgtaa atatgccaca tatatctaag tattttaatt taaatgcctt taaaattgac
    44461 ctcacatcat taacatcaaa acccagtctg tgcatacctg tatcaatttt aatgtgtgat
    44521 tttatgttgt atttctgttt gtttaaagag atagcatgat taaggtcaat aacactctgt
    44581 gtaagattgt atttatgaag ttcctttgcc cttattgggt tagtatatcc aagaatcaat
    44641 atttctccta gtattccata tcgacgtaaa tcaatcccct catctattgt tgctactgca
    44701 aatgctttta cacctatttg gtttgcacaa gtcgctactt caaaagcact atgaccatag
    44761 gcattagctt ttacaactgc catcaattca caatcttctg gcatagcttt ttgtatcatc
    44821 tttacattat gttccaaatt atttaaatca atttcaatcc aagctctatt ggtatttacc
    44881 ttatgttctc ctttcaagtc tttttttaga ggtttaagaa tcaatataat aattatagaa
    44941 gctataaaag atgctatact taccactata taatgaataa ggctattgtc aaccaagaca
    45001 gtttgtattc cgaatatttt agaaaacata cgaataacaa caatcatcat aggatgtata
    45061 atataaataa ttaatgatga tgttcgcaat aacctagctc gagaaccttt caaaaatgtt
    45121 aaggctgtaa ataagaaata catacatggc aataacataa tatacatgct atcatgacgc
    45181 tgtaccccta gtttatgtaa aaacattccc tcacataaca taagtaagaa tgatataaga
    45241 aatccaatca agctattttt agttgacata taattagatt tattagatat tattccacca
    45301 agtatgaaaa atacaggtgc aaaaaatata ccatttcgtg tataatctga tacttcaaaa
    45361 atatacccat ataaattctt taatagtggt attttttcta aaaaaccata ataactatca
    45421 ccaaacatac caattacata aataacaata gtgattacta gtgagtactt taaacctagt
    45481 ctttttacaa gaaagtatgc aatacttgca ccaataattg atgcaggtag ataccataaa
    45541 tgatataaag tcccatcaaa tacaatgtcc ttaataatat tgggcactaa attattcatg
    45601 gaaaaatatc cagtgtaaat atttaaggga atataaatta caatagctat cccgtaaata
    45661 aataatacat tttttagaaa agatttcagt ttattacaat tataattata tgttgaaatt
    45721 agaaaaaagc ctgatagcat aaaaaagaat ggcacagcaa tacgagcaat tatactagtt
    45781 aacacaaagt cagccatttt actatatgat aataatggag atgtatgaat tgcaattata
    45841 agtaatgcag ctataatcct aaaataatca agccctgcat aagatttatt cctaatcatt
    45901 cttgttcctc catttcttag tcctttctat ttctccaaaa tgtcacaata aaaccatcct
    45961 tattgttacc cgatatttga tatacacatt gttcaggtat agagattctc acttcttctt
    46021 tagtatgaat aggcacataa aagatttcta taattttttc tttatcttct atttttaaac
    46081 aatttgtata cgaaaatctc ttaatatatt ctatatattc ttcaagagcg agattcattt
    46141 tattgataat ctcagaatgc ggatatccca cataacgaaa atgccatggt tcttgagata
    46201 ttcctgttat ttcttctttt ttacactgat aacgctctat aaaaccataa tgtagtgctg
    46261 ccttacgaaa ctcattacaa ataccattat aaggaaattc agggcagata aaatctataa
    46321 cttctttctt taaccctaaa tcaatagcaa gccctgtctg atgttcactg tgatttggaa
    46381 gtgctacaaa ctttttagta aagtcttttc cattgtcacg cagagaactt gaatatatat
    46441 cttgttgttc ctctgcagta cgatatccac ttacaggtac tatttcattt attccacata
    46501 tactttttaa aatattttgt aaaatgtttg tagcttcacg tttcataaga atacttggaa
    46561 accttgtatc tacaggaatt aaatctatat cttcagatgt tttaactggt aaaaaagcat
    46621 ttactagaat tagattccca tcataaactt tatctttagt caataagata gtatttgtca
    46681 ttttatcttc tttattttta tctaataaaa tattattcat cattttatgt acacctcaat
    46741 tatcttgtcc aatatttctt caaaagacaa tccaatacct ttcatcatat ttggataacg
    46801 actatgagaa gtaaagccag gaattgtatt gacttcatta aaaataattt caccagatgg
    46861 agttaaaaac atatctactc tagcaaatcc agaacatcca agagttctat aaataatttt
    46921 tgctgtctct tgtattcttt tctcacaatc caaatcaatt ctagctggca tatgaatttt
    46981 tgaacttttc aatgtatatt tttctgtata atcaaaaaat ccttttgaca attctatttc
    47041 atctactctt ccaacaagta agtcatcgtt tcctaaaact gcacacccta cctcaaaacc
    47101 atcaatattt tcctcaatta ttacttcatt atcatgttca aaagccaagt ctactgctac
    47161 ttgtaactct tcttttttat aaattttact aataccaaat gatgaacctg cacgcacagg
    47221 ttttataaat aatggataag ttaagtcttt tgacagttca gccacattaa atttatctga
    47281 gtattttagt aatattgatt ttggtacttt aataccttct gaatgaacaa gtttatgtga
    47341 tttgtctttg tccatacata atgctgaaga aagagtacca catcctataa cctgtatacc
    47401 tgccaattca aataacccct gcactgttcc atcttctcca ttttttccat gcagtacagg
    47461 aaacacaaaa tcaagtttag tgtatttaat atcattttca catgattcaa tagcactatt
    47521 tggaatatga ttctgagaaa cagcatcagg aattaaaaat tcaataatac catttttgac
    47581 acggttctgc gaaacaataa ctggaattat atcagtatcc ctttcaaacc aagtgttatt
    47641 ctggatatta ttataatctc caatataatg ataccaatta ccctcccttg taattccaat
    47701 tggtataata tcatattttt ccgtattcaa attctcaagt actgcaaaag ctgattgtaa
    47761 tgatacatca tattctgttg agtttccacc aaatataact gcaatttttt tattattcat
    47821 ctttgattct cctttgttga tttatttatt tttatacaat ctacagaaac atgttaaaat
    47881 actttttcta tttcaaatcc aatcaacctt cacttacaat tgcatctgtt ccacataata
    47941 tattttcaca tttttctatt gatataatta ctgtatcaaa ataaaagcac tcttgttttg
    48001 atacagtaat tatatcaata acaagagtgc tctttattta tttatcctaa agaatttcct
    48061 atttttttat acttaaattc ttacaaattt cctacaatcg gaataataac cttaaagata
    48121 attatattgt tctcgctatg agctgttatt gtcccgttat gaagctccac tatttcttta
    48181 gctatagcaa gtcccaaccc agctctacca ctgtttgaat tacgagatgt atccaacctg
    48241 taaaattgtt caaaaatacg ctcaagtttt tcctctggaa tcgtattgct atgatttata
    48301 aatttaagct ttatataatc atcctcctgt acagctgtta tttcaatagt agtatcttca
    48361 aaactataat aaacagcatt tcgtaataag ttatcaaata cacgttgcat tttattaaca
    48421 tcacacctta tcatcatatt tggtgtaaca ttcagtgtat attttaaatt cttactcatc
    48481 aaagtaggtt taaactcata tgttagttgt tcaagcatac gagttaagct tactgtactg
    48541 tattcaagtg atatatttga taaattaaac cttgtaattt caaagaattc attaattaaa
    48601 tcttcaagac gctctgcctt atccaacgaa atagataaat atttttttcg cagttcttca
    48661 gaaatctgac tttcatctcg aagtagtgtc agatacccta taactgatgt aagtggtgtt
    48721 tttaaatcat gtgcaagata aacaataagt tcattcttac gttgttctgc catttgtgtc
    48781 tcaaatttac gttgttcaag tatatgttta attgagttta ttttcttttc tattgccaat
    48841 aactctggag ataaagccac ttctccaaca tcttctttga taagagagtc aataccttga
    48901 tttatttcac taaaatattt tgtaaaccaa tttaaataaa cccggaatat tataaaaaac
    48961 acaatcacca tagctagtat catgaagagc tccatgctat tacggaaaat ctgctgatat
    49021 aaattcaatg cagctttata ttccatacga aaagcatttt caaagaatga tactatccag
    49081 atagaaaact taccatacaa tataaaagaa tataaaatcc atatgaaaat aatggctata
    49141 aatgccatta aagctgttct cataaaaatt ttatttctca cttgagtaaa gtcaggattg
    49201 actttaatat ttttactttt caattgtata acctactccc catacagttt ttataaattt
    49261 cgggttttta gaaggttcat gaattttttc acgtaatcgc cctatatgag ccataactgt
    49321 gttattatta tcaagatatt tttctcccca tacagcttca aacaattctt cagaaggaac
    49381 cacttttcct tgatgttcac ataaatacca aagaatagaa aattcaatcg gtgttaatgc
    49441 tacttctttc ccaaataaac tacatttatg tgtttcttta ttgataatca atcctttaat
    49501 atcatactca ttaacaatta tcgactgttg ttcataagaa ttattatatc gcatatatct
    49561 tcttaattgt gtctttactc ttgcaacaac ttccaaaggg ttaaatggct ttgttatata
    49621 atcatcagca cctatggtta aacccattat cttgtctata tcttcaattt tagctgttaa
    49681 cataatgata ggataatagt atttttctcg aattttctgg caaatatgaa atccatcaat
    49741 atcaggaagc ataatatcta aaatagccat atccaaattt ttagattcaa tacattctag
    49801 agcttcctta cctgtataaa acttgtatac tgtaaaacct tcatttttta aatatacttc
    49861 aattaaatct gctatttctt tttcatcatc cacaatcaaa atattttcgt tcatagatta
    49921 tttttcactc tccttattat cgaggtcatc atttataata ttggtaaatt tacatatcaa
    49981 ataattacaa gcatatttgt aactaatttt tataagaaaa gaactactac atcagaatgt
    50041 gttgatgcta ctattggaac acaaaaactg agattaattg ttattataaa aactccccta
    50101 acacgtcctt attatcgcat aacaggtatt aactttcaat aaaaatatct atcagtcaaa
    50161 cacaattcaa tttattatac cagagacaat ccatataaaa aacaattaaa cctatgtatg
    50221 taaccacaga cattactaaa atttccatgt tattttatat aaaaattcaa aaaaggagtt
    50281 gaatttaaaa tttgattaga aaaaaatcaa ttttgagaca actccttttt attttaatgt
    50341 atttaaatta tagtatatca gccttcataa tatcctcaaa atcacctttt tcagatatta
    50401 atcttatcaa tttaacagta cagcttaaat catttataag tatagctcca actaatgaac
    50461 catctttcat aaataatttt ttatatattc tttgaccttc atcttttgat gaaatttctt
    50521 tatcaaaatt ttgtatatta ccaatactaa atacttttac attcattgca tctaatgaaa
    50581 ctggatatac ttcactttca aatgtcaaat tatctccact tgcatttgcc cctgctattt
    50641 ttcccatttc tactgaagat ggccatattg ctaaactaat atttcccact tgtgcaacat
    50701 ctccacaagc atatatatct ttaacatttg tctgtaatgt cttatctaca acaatacctc
    50761 tatcaatttt tacagatgta tcctgaacca tttgagtgtt tgctcttaca ccaattgaaa
    50821 atactaccat atcacagtcg acactatctc catctttaaa tacaactttt tgagcttttc
    50881 catcaccttc aataccatca actgctacac ccaatcttac atctattcct gtatctctta
    50941 cacattgttc taatatttta ctaccttctt tatcaagttg ttttgatagt atgctatcca
    51001 tggcttcaac tactactact tctaatcctt ttagtctaaa ttcccatgca gcttcaagtc
    51061 ccaataaacc gccaccaatc actacaactt tcttagattt ttctagtgct tcttttacac
    51121 tatataaatc ttttatattt ctaagagtaa acacattttc taaatcatgg cccttaattg
    51181 gtggtataaa gtttctactt cctgtagcta ttacaagctt atcaaattta atagctccat
    51241 cattcactat aatagtttta ttcacttcat ctaatttatc tacattcgtt cctaatatta
    51301 ctactatatt atttttatca taccaatctt tatcttccat gtagaaatct gaaccaagtt
    51361 cttcatttat accatcagaa agtgcaggtc tatagtaagt caactcatct tcattagaaa
    51421 tcatagttat cttacaagtt ttatttcttt ttcttatagc atctgctgca taaaaaccag
    51481 ctgctccatt tcctactatt acgaaatatt catcagtatc ttttctaaaa gtaacttcat
    51541 cttcttcaac ttctataaat tggtcttcct tagctccaca tacagggcat acactaggag
    51601 cactatcacc ttcaaaaact tcattacaaa ttacacattt aaatttctta gttttctttt
    51661 tacttccaaa agtagaattt aacttatcta ggaatttacc tgtataagaa tataatttat
    51721 caatctcttc atttgatggt ttaaagttaa cagcaaaagg ttttactgct gtcattctaa
    51781 gctgacttat tctctccata gcattttcta taccttctcc actccatcca tatgaaccaa
    51841 atactgaagc aacttttcca ccatgaagaa ctgggtttaa cgatattaat atatcccata
    51901 taggctttaa cgcatctcct aatattgttg gtgttccaaa taatacacct tgtgatactg
    51961 ctatttttgc caaaatatct tcttgcttat gctctattac atcaaataaa tttactttat
    52021 agttgctatt tttttcaata tatgctttta tagcttctgc cataatcttt gtatatccat
    52081 gtgcagaaac ataacaaatt gtaacttctt tttctaactt tatttgttca tttacactcc
    52141 agttgtaata aaggtctata atttttctag gattttcagt taatactgga ccatgacctg
    52201 gacatacagt atctatatct aaatctttta tcttttctat agcagttacc atagatggtt
    52261 taaatggacc cattatacaa tcataatagt atcttaaagc atccaagtaa tccttttctt
    52321 cattttcatc aagtgtattt actatcttat cattactata gtgactacca aatgaatcac
    52381 atgttacaag agttttatcc tcttttatat atgtgtatat ggtatctggc caatgaagca
    52441 taggtacaga gaaaaattct aaagttttgt ccccaattga taatgtatct ccatctgtaa
    52501 ctgcaacata ttcaaagtct ttgtttacga tttccttaag atatttaatt gctgtttctg
    52561 aagctactac ttttgcattt ttagctaaat ccaaaagctt ttcaacgctt cctgcatggt
    52621 ctggttcagt atgacttaca acaatatagt ctattttttc aaaatctata tttaaatcat
    52681 ttaatctttc aatataattg tcaaaatgct tatctttaac tgtctcaaat aaaactgttt
    52741 tttctgttcc ttttaataca tatgaattat aagtagttcc ataaggtgta tacataatta
    52801 tgtcaaatac tcttaaatca gggtcaagcg aacctaccca atataaattt tcttttattt
    52861 ttagtgattt caatgacata ccgtactcct cctaaaatag ttatttagtc tctttatacc
    52921 ctgcttgtta tattttaaac aaaatttatt caaaaaagaa attattttaa agatagttta
    52981 aatcttctaa tcttcttttt ctaccttcaa tacagttccc tttttagaat ctacttttat
    53041 gtcatattca tttttatctt tatctattct tattttataa aaagtaacat ctaaatcttt
    53101 gtctaaactc cattctttaa ctattccatt ttgttctttt aatgcaattt ccatagcttg
    53161 ttgaggtgtc attatgtcat ttaaatctaa tttttctttt ctagcaactc catttaaatc
    53221 ttctgaatcc aacttttctg ttttatcttc taatacatct tttgtattag catcaatttt
    53281 catcttatat tcattattat catctacacc ttccacagta taaacaaatt tatttaaatc
    53341 tctctcaaga cttaaatcta ctatatcagc atccttatat ttatctttaa atatgtttat
    53401 tgcatctgtg tatggcactt taaaatcttg ttccattaaa ttttcattat ttttttcatc
    53461 ttttaaagcg ttatttgatg catttgcctc cttttttgta ttatcttgtg cttgtcctga
    53521 atccttagtt ggactcccac tacatgcagt taataaaccc gctataagca tacttaccac
    53581 taataaatac gtttttttac acataaaaac cctccttaaa tatattaatt tattctatat
    53641 tatgccctct aaacccttta tttacccttt tatattaaaa attcctccta ttttatcaat
    53701 tttaaaactt tatatttact aaaattaaag ttacataaaa attatactgt ctcaaaatgg
    53761 aaacaagcct ctttttcatt gttgaaataa taaaaatata ttttatttcg tacaaaaaga
    53821 gtgcctcatg aactaaaaag ttcattttga gacactccca tttttttata tttttactta
    53881 caaaataaca gtacttatta attttttaca aaccttataa gtatatctat atgatattct
    53941 taaatttaaa cactatatta aaaaattaaa ttttgtttaa ttcttttacc gttaaatcca
    54001 ttttaggata tggaatcgta attccttctt cgtcaaacct aactttaacc tcttctagta
    54061 aatcaagata aattgtccaa taatcttctg tcttgcacca aactctagtt gcaaatttaa
    54121 tagcattatc tccatgttct actactccta taaatggttc tggttcattt attattaatt
    54181 tatgattatt aacaatatct ttaagtactc ttctaacatg tagtatatca tcttcatacc
    54241 ctacactaaa agtcaaatca actcgtctag tattctttgc tgaatagttt attagactat
    54301 catttgatac agaaccatta ggtataagta tctgtttatt atctggagta accaattgtg
    54361 tataaaaaag acctatttgt tcaaccgttc ctccatgacc tgctgcttct acgtaatctc
    54421 ctactttaaa aggtctaatc aatagtataa taaatcctcc agcaaaattt gatagactac
    54481 cttgtaaagc caaacctatt gctacccctg ctgatgctat cacagcagca aaactggaca
    54541 aactcattcc ccaatattcc agaacaatga ttataacggc aactttaagt acactcaaaa
    54601 gaagagactt taaaaacttt cttaaagtta aatcaatatc tcttttacta agtaataaca
    54661 taacatggtt tacaaatttt tttattattt taaatccaat agaaagtata agtatgccta
    54721 ttataagttt tactccgctt gtagtagccc attctagcag actttccatt atacctccca
    54781 tatccatctt attaatatct aaattattta aatctaactt gcttatatca tctattttcg
    54841 tcgtatccat aaaaaactcc ttaccttata ttttagaacc aatattatag tttaaagttc
    54901 ttattttcta tttaatataa tacaatacca tttgtacata gtaaacaaga tttatattcc
    54961 aattatacat attaggaaaa tgcaaactat ttgttatata tatgcaaaat ataatactaa
    55021 attaaactat taataaaaca ctatatagcc ttaaaatcat cattttttac ccctatatga
    55081 gatgatacta tagttataat acttagtact ataaatacta caaaaccttt tttatatcca
    55141 tcaactatat tatatgaatt tacttgtaaa gagatagcta taatcaaatt aataattcca
    55201 cttcctataa attcaaaagt attaataaca ctagttgcca gtccagaata aatttcttta
    55261 tttttatact tggcatcatt aaaaacctgt agatgtgaca taactataca tcccataata
    55321 aagaatgata ttggtaaaac tataattggt ggttttacct ggtatacaac tacaattaca
    55381 atccatataa acaaatctat cattgctcca aattttatta tattaaattt gctagacctt
    55441 attttagcaa atacaaaatc cataattatt gaaccaaata taaatccata tgtaaaaaat
    55501 gatactataa acgcagaaaa actcttactc acaccataaa cgtccattat gtatcttact
    55561 ccccacaagc ttataaatgc acttgtaaga ccaacaaaag agaacattat cattgaatta
    55621 taccatgttg atttgttctt tataacagac ttaataccat caacaatatt aaccttctct
    55681 gacttttcat agggttcagt atctacattg aatccatact cttttggtgt atctctaaca
    55741 ataatataca taaaacatcc aattacaaca ccaattatac cgattaataa aaatgaattt
    55801 ctccaaccaa caagttcaga caaaaacaca agtggaaaag tagcaagaac tccacctaaa
    55861 tttcctacaa atgaaaattt agctgttgca gaagcaaatt ctgacttatt aaaccatctc
    55921 ccttgaattt ttaaaataca aagtaatatt actgatgttc ctgcccctac aattacacga
    55981 gaaatatatg ctaattctat actttgaatc aaaccaaata aaattgaccc taaacttgcc
    56041 attattattc caagactgct aatttttctt gctccatatt tatcaatcaa aattcctgca
    56101 ggtatttgca taaatgcata tgcatatagg caaaaggatg ctatatttga aatttgtata
    56161 gaattaaaac ctaactcatt tgctaaattg tctgaaacaa cagcagttga catccttaaa
    56221 aataatacta aaacaaatga aaatgtaatt ataccccaaa cactccactt agcctttgta
    56281 taacttttca tatcttctcc ctcaaatcca tatattttta aattttcttg atataactta
    56341 tataaagtgt atagttattc ttacaaattt ttacacgcaa tatcattata caacatatgt
    56401 atatatttaa ctagaaataa aacactattc atattatata catgaattaa ctacataaat
    56461 aataagagcc atcttttaac attactcaaa agatagctcc atataaaaac atttattcta
    56521 aatttaattt tttatctaat ataaattttg tagcgaaaaa taacgctatt gcaactacaa
    56581 gactaccaag tattgcaagc ataaaactag gactctgata aagatgatat accatatttg
    56641 tatcttcatt tggtaagatg cttccaactg catctccaac aattgatatt actatattta
    56701 tagctataaa agaagcaaat gccactatgt ttctatgttt attgaatttt ggcaattgtc
    56761 ccattgagat tgatgtatat aataacaaaa taaatgatga atatgaaata aacattacta
    56821 ctactaataa taaaatatct aaaaagtttt cagaaatcca tttaaaacta gtgttaaaca
    56881 aatctaaaat ttcaggtagt aaaattccag aagtaccaaa aagcataaga actccaaaag
    56941 tgaatactgc aactataaaa ctaagtacag cgtatataag tgctgttata catttagata
    57001 atataagtga actagtgcta acaggaagag taaacattaa ataaccttca tcccccaaaa
    57061 gattttttct aaacctttga attgtaacta caatagttaa aactcctaat gccataaaaa
    57121 gacttgttaa tactagtatt cctattccct gtacctgaag tatatttgta ttcataaata
    57181 ttccattaaa tacagctact atcaatatgg ctatatataa cggtataaat attcttccac
    57241 ttgcttttaa ctcatatttt aataatttac ctaacattta aattcctccc taaataatgc
    57301 atctatagat tttccttttt cttctcttat tgcttcaacg tctccttgta acactatttc
    57361 tccttttgat atgaatatta tctcatcaca tatgttttct atttcactta ttaagtgggt
    57421 agcaatcagt aaagtactat cttcagaata attttttaat atagtcttaa gtatatatga
    57481 ccttgcagct gggtcaacac cacctatagg ttcatccaat atatatatgc tcgcatttct
    57541 tgacattact aaaataagtt gaactttttc tttagttcct ttagacattg tctttagctt
    57601 ttcatttaca tcaatatcta agcttttaat catctcattt gctcttctaa catcaaaatc
    57661 actataaaaa tcatagaaaa acttaagtaa atctgatacc ttcatccaat catttaaata
    57721 agttctttct ggtaaatatg aaactatttt tttagtatct atggatggtt tcattccatt
    57781 tatcataatt tcaccattat caggctgtaa taatccattc attagcttaa tcattgtact
    57841 tttgccactt ccattaggtc ctaacagccc gactattttt ccctttggta tatttaaatc
    57901 aatatttttt aaaacattct tagttccata actcttgttt acacctttaa attcaactat
    57961 tccatcttga gaaacattgc ttatattttt atccatttta taattctcct ttcagatttt
    58021 ctgtaataag ttttagcatt tcatctctat catatccaag cttttctaag gttactttta
    58081 atgtatttat ctccacatta gctatttcct ctttcatagc ttttattttt tccttatcat
    58141 ctgttacaaa tcttccttta gttctctgac tataaactaa accttgtctt tcaagttctg
    58201 ttagagcctt ttgcattgta tttggattaa cttccgcatc ttctgctagt gctctcactg
    58261 tttctaattt agatcctatt ttaatttccc ctgaaattat ttttaacttt aaatgttcta
    58321 ctaattgtat atatataggt ttgttattat caagttccca ttccatgttc atgcctcctt
    58381 attatattat tgtattaagt tgttaataca ataatataat aatacaaagt atatgtcaat
    58441 tactttttag taaaagtata aaaaatttta caaaaaaaat gaacactaca ttagagtcca
    58501 tttttaacac aatttattaa attctttata ttacagatta attaccctct taccaagtta
    58561 gatacaatta aatgttcaaa ttcttcaatt ggcattggct ttccaaacaa atatccttgt
    58621 gccatatcac actttacttc ttttaaaagt ttaaactgtt cttctgtctc aactccttct
    58681 gatatgactt tcatacccaa ctcttttgcc atttctatta ctttagtaac aattattttg
    58741 cttcttttac tattataagt ttccataata aatttttggt caagttttaa gatatccata
    58801 ggaatttctt ttaacatatt taatgatgag taccctgaac caaaatcatc catagatatt
    58861 aaaaatccta ttttttttaa attattcatt atatcaatta aaatatcaaa attatcaaat
    58921 atgatactct ccgttaattc aagttctata tactttgcag gtatattgta tttatcaatt
    58981 atatttttgt atgtttctat aaaattattg caatataaat gtatccttga aacattaact
    59041 gaaacagtaa ctggatttat tcctgcatca atccattttc taaggtatat acatacttct
    59101 tcaagcacat ataaatctaa atttataaca aaaccgtttc tttcaaaaaa tgggataaat
    59161 tcatttggag gtattagccc tttatcagga cgtttccacc taacaagtgc ttctgaacct
    59221 tgtatctctc ctgtattaag ttctatctta ggttgtaagt atataataaa ttctttattt
    59281 tctagacttg aaaacattgt attttccatc tctttttctt ttatctcttg gtcatgaatc
    59341 ttattatcat aaaatgcata tgaactttta tgtcctccct ttgccatttt atgaggtata
    59401 ttagccctat ctatgattga gttgatatct ctatcttctg gaagtataaa atagattcca
    59461 caacttaaaa ctaacttata tttttcttct tgattattat taaacaagtc taattcttca
    59521 aatatagttt ttaacctctt tgttatatct tcttcttcta tatatttcat aattataata
    59581 aaattatccg cagatattct tgcaaacatt tcctcttcaa atatattgct cttaataatc
    59641 tttgttaaat tacgcaaaat ttcattccca acttcatatc caaataaatc atttatatat
    59701 ttaaatttat caatatctat atataataaa gcatacttta cttgagtatt tttacatagt
    59761 attttgttag catcaattac aaatttatct atagtatttg ctcctgtaag attatctgta
    59821 tatgctatcc tttgcaattt aattttaaaa tttaatttat catatattat atagaatata
    59881 agtattacac atattatagc cataataagt attatttgta ttgcatgttt gctaataagc
    59941 tgattattag acacaataga ctcaaagtta tttatggagt tgtttttata gagtttgcat
    60001 aaagaataga ctggtgttgt gtatgtaatt tgtaaaatta tacaaataaa taaaaaaaac
    60061 atcaattttt tttgtaaatt cctcataaaa atttcctccc attaaaacac ctctatttta
    60121 ttagcaaata agtacatctc aaaagtttcc atttcttgta aaatggatag ttttgaaaag
    60181 aaaatattaa aattataata taaaaataaa atttttcaga caatttaaac aaaaataatc
    60241 accttttaat aaataattat atgc
//
LOCUS       DAYAPA010000007        59033 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.007, whole genome shotgun sequence.
ACCESSION   DAYAPA010000007 DAYAPA010000000
VERSION     DAYAPA010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 59033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59033
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.007"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            201..1034
                     /locus_tag="ACGI59_000357"
     CDS             201..1034
                     /locus_tag="ACGI59_000357"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFG9738170.1"
                     /translation="MRNKKKKINRKKLYLLLSAIVVCLAFIVVGVRVSSLNMKENEIS
                     RNAKLSSSTITDIVSNTSIEVSKGPSKSSGKIAYITIDDGPSKFTDQMIKTLNKYNVK
                     ATFFMIDGNMKEYPQQVKNIIKNGNTAGFHSVSHDIHKLYVTSTSAKEEFDTNDQTFY
                     KITGKHSKVIRIPYGSKPYTPQASYQALVDAGYKIWDWDLDTEDWRSNSSQIVQNVKN
                     HIKNRKGEDKDQLVVLMHEKKQSAEALDSVLKFLSDEGYEFAPVDQNQIPKNYWLRNL
                     E"
     gene            1103..2059
                     /locus_tag="ACGI59_000358"
     CDS             1103..2059
                     /locus_tag="ACGI59_000358"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFG9738171.1"
                     /translation="MNKKLYIGMVGILSLMMVGCNKSLAKVNDVEITKEQYKKTKAVL
                     SATNNYINGQSLDELEKTLDKKGRNKLENVIISFMVDNELLYQEAKDKGLTPSKSEVD
                     SKYQELEDKMNLNTSYKEKMDKAGVDKDYLKQEISRDLAIDKNKKAFEDRINISDNDM
                     EAYYTSHKKDFNVEEVSASQILISTLDKNKKEVSKDKKEALKKKADNILTKIKNGESF
                     ESLAKKYSDDKATGKNGGQLGYFTKDDKNAEFTKEVFKLKKNEVSNVFETSYGYHIVK
                     VTDKRERQKSFNECQSLIRESILNEKYIEHIKKLNEDAKIDR"
     gene            2111..2593
                     /locus_tag="ACGI59_000359"
     CDS             2111..2593
                     /locus_tag="ACGI59_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFG9738172.1"
                     /translation="MFKSLKRTKVEKYIIDNKDSFYRIAYSYTKNEEDALDVVQEAMY
                     KALYSVENIKEVNYIKTWFYKILVRTSIDFIRKNRKYNNMTDIDLIDETGEYDKYTDL
                     DLRRALEELPIEYKSIIILRFFEDLKIEEVAIILDENVNTVKTRLYTALKKLKLKIEE
                     "
     gene            2605..3474
                     /locus_tag="ACGI59_000360"
     CDS             2605..3474
                     /locus_tag="ACGI59_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HFG9738173.1"
                     /translation="MTSKERLNKLKDEYHKIPIPKKLDTIINHEKIENIYREKNKKVN
                     RLRFKVAIAFACIFTVLVNISPVFADNFSKIPVIGAIVEVITIKNYSLKSENYEAEID
                     IPKIRGLKDKNLEQRLNSSFMEDGKRLYHQFQERMEKIQSSKNKGYKSLSLSYSVKNN
                     SKKFLSIEMTKNEIEASSYVSKVHYTIDKKRQIVLTLPMLFKDDKYIKVISDNIKEQM
                     REQMKKDSTKSYFIDQKKDLPVEDFKTINKYQDFYFNKNEDLVICFDEYEVAPGYMGA
                     VEFVIPYKVIKDL"
     gene            3517..4272
                     /locus_tag="ACGI59_000361"
     CDS             3517..4272
                     /locus_tag="ACGI59_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HFG9738174.1"
                     /translation="MTTYKKLKKSIFKIAIFSICLLASLVDTNPIFAGSSFNETSNSD
                     TIKVLNAKSINFKQDNYEVNVSIPKIKGLKDKTLENKLNNEFLQDGKKIYSEFQNKMK
                     KEDNKGHRYLNVIHNVKNNSKDFLSIEMTKEEIEASSSITKKHYTVDKNRGILLNLPI
                     FFKDSRYIRAISDNIKEQMRNQIKKDDTKSYFIDQDKDSPVKDFESINKYQDFYFNKN
                     GNLVISFDEYEVAPGYMGAVEFVIPNKVIKRLR"
     gene            4314..5135
                     /locus_tag="ACGI59_000362"
     CDS             4314..5135
                     /locus_tag="ACGI59_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HFG9738175.1"
                     /translation="MNIYEQAIRLIENNEDFAFATITSHSGSTPRETGAMMIVKNDST
                     IFGSVGGGSVEAECIKHAINVIKNRESMLYKFTLNKSDVAKLGMICGGTGEIQIDFID
                     SKLKSNIEKFNKRLKENTSKAYIFGAGHISRDVAVILSLLEFRTVVIDDREEFANHER
                     FPESEVVVLDSFENIPDFPTDENSYIIILTRGHLYDSSALEWALKREAGYIGMIGSRT
                     KIGLTYEKLMKKGFKKEELSKVHAPIGIKLNAQTPAEIAVCIAAELINCRANKEK"
     gene            5140..5901
                     /locus_tag="ACGI59_000363"
     CDS             5140..5901
                     /locus_tag="ACGI59_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HFG9738176.1"
                     /translation="MNTGSKDIDTLKVNFRGIEIKAVLVYKNRKNISIKIDPFGKITV
                     MSPPKISKKIIKDIIIEKGDWILKKSEKCKGREEVYKQRMFITGEKFLYLGEEYHLVI
                     KELLRDSVKKSNCRITINEYQIVVQTNDTSTDFIKKSLKSWYKMESERIVLERIDFLK
                     LNCEIMKQLVPASVKVKEQNKRWGSCTAQKNIYINSKISMARLDVIDYVIIHEFSHLV
                     HMNHSKKFYDLVKSIMPDYKDKENWLKINGYKIII"
     misc_binding    6062..6319
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            6432..6671
                     /locus_tag="ACGI59_000364"
     CDS             6432..6671
                     /locus_tag="ACGI59_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HFG9738177.1"
                     /translation="MENTVYAKLNQGIDSFLRVAMTLRRREVDIQSISMTVDNMNNSG
                     IKLIVNEEKTSLDSVLNHMKKLHDIREITVERMRH"
     gene            6924..7970
                     /gene="ilvC"
                     /locus_tag="ACGI59_000365"
     CDS             6924..7970
                     /gene="ilvC"
                     /locus_tag="ACGI59_000365"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HFG9738178.1"
                     /translation="MSNKNKYHKNKKKYWEEMKMARMYYEKDVDLEVLKNKKVAVLGY
                     GSQGHAHAQNLRDNGVHVVIGLYDGSKSAQKAKEDGFEVKSVAEATKESDLTMMLMPD
                     EKQKKVYEESVKDNLKEGQTLAFAHGFNIHYNQVQPPEFVDVVMVAPKGPGHLVRNVF
                     TKGSGVPALFAVYQDYTKKATETVLAYAKGIGATRAGVLETTFKEETETDLFGEQSVL
                     CGGISELIKLGYKTLVDAGYQKEVAYFECLHEMKLIVDLIYEGGFERMRYSISDTAEY
                     GDYVSGKRVITDVAKQGMQNVLEDIQNGKFAKAWIKENEEGRENFLKTREEEYNTEIA
                     EVGRNLRSMMSFLK"
     gene            8023..9711
                     /gene="ilvB"
                     /locus_tag="ACGI59_000366"
     CDS             8023..9711
                     /gene="ilvB"
                     /locus_tag="ACGI59_000366"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HFG9738179.1"
                     /translation="MNGAKVILECLKKEGIDTIFGYPGGAVIPLYDALYDYSDDFKHI
                     RTSHEQGLVHAADGYARSTNTVGVCFTTSGPGATNAITGIATAFMDSSPMVVISGQVP
                     TSLLGKDSFQEIDITGATLSMTKHNYLVRNTKELVPTIKEAFRVANSGRKGPVLVDVP
                     KDLFLAEMDFSGEDYDLCQIDDYMDYKSDFDLDDETNIKLLNEAIDIIKESKKPIIYA
                     GGGVKSSDSEEILEKFATKIDTPVLNTLMGLGNIDRKNELSLGMVGMHGSRESNLALS
                     NSDLVIAIGARFSDRVISKSSEFAKNAKIIHIDIDPSEISKNIESNVSLVGDVKLVLS
                     LLIERVESKNNSNWKEEIKRFRKSEGVQTYEFHPQNILKKINEKYETLKKPTVVVTDV
                     GQHQMWAAKYWNFKGNKSFITSAGLGTMGFGLGAAIGTKVGNVDKNVVLVTGDGSFRM
                     NCNELATVANYNVPMLILLLNNRTLGMVRQWQKLFSNQRYSQTDINENVDYVKLVNAY
                     NIDGYKVSSMEELGKALDMIDFNKPVFLQCDIDKDYDVYPIVAPNDALENLICN"
     gene            9922..10599
                     /locus_tag="ACGI59_000367"
     CDS             9922..10599
                     /locus_tag="ACGI59_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HFG9738180.1"
                     /translation="MFKHDKALLKEVKVERPNPQYAVLMQEQLGGANGELKAAMQYLS
                     QSFRIKDPQIKDLFLDIAAEELSHMEMVAQTINLLNGHDVDYNAVNTGEIETHVLTGL
                     SPVLINSSGAPWTANYVTVTGDLVADLLSNIASEQRAKVVYEYLYRQIDDKYVKETID
                     FLLNREEAHNALFRDALNKVKDTGSNRDFGVTEDSKLYFDLSTPGPNHDTKIDINPPS
                     FEKPLKK"
     gene            10768..12804
                     /locus_tag="ACGI59_000368"
     CDS             10768..12804
                     /locus_tag="ACGI59_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HFG9738181.1"
                     /translation="MDSNRKPNNLINEKSPYLLQHAYNPINWYSWNDEAFKKAKEEDK
                     PIFLSVGYSTCHWCHVMEKESFEDEEVAEIMNRNFVAIKVDKEERPDVDSVYMTVCQA
                     MTGSGGWPMTIIMTPDKKPFFAGTYFPKYSRYNRPGVIDLLENVSEKWNTSRDILIKS
                     GDEIIEALKDDFGVKNTEGDLSKEMLSSSVRVFKAIYDEKYGGFGNAPKFPSPQNLMF
                     LMKYYSIEKDKDVLKMVEKTLDGMYRGGLFDHIGFGFSRYSTDKKWLAPHFEKMLYDN
                     AMLTIAFLDAYKITKKELYKEIAIKTIDYVVREMKDKEGGFYSAQDADSEGEEGKFYT
                     FNPLEIIEVLGEEDGIFFNNYFDITSSGNFEGKSIPNLIKNKEYERHNEKIADLSKKV
                     FEYRKERTSLHKDDKILTSWNALMIVALTKAYSTLKNDIYLEYSNKCLNFINNNLVNE
                     SGRLLARYRDGSSDYLAYLDDYAFLIWAYIELYESTFNMKYLEKALNLNESCINLFWD
                     YEKSGFYIYGKDSENLIARPKDLYDGAIPSGNSVQLYNLIRLAKITGDNRLEEMSYKQ
                     LKLYVDNVKSSPAGYSFYMLSLMFELYSTKEIICIFKEDSDLIAFKELISENFIPNAT
                     FLAKKYNEENTIIGFLNNYRLKDDKISYYVCQSNSCSQPINDLQKLKDMILGVE"
     gene            complement(12995..13561)
                     /locus_tag="ACGI59_000369"
     CDS             complement(12995..13561)
                     /locus_tag="ACGI59_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFG9738182.1"
                     /translation="MDYSNELKELFLMNQTYATLFTLTNKIQIEGDKYFGILTSRQYM
                     TILSILHLPEEETTLNNIARKMGTSKQNINRLVANLEKNGYVDVIPSPHDKRAINVKV
                     TDLGKKVMVTCSRTGINFMADVFHEFTKDELETLWSLLKKMYRFNGEEQDGFEEDANF
                     MEYEEIDKIKSEALEEFAKRRKRVNKND"
     gene            13849..14784
                     /locus_tag="ACGI59_000370"
     CDS             13849..14784
                     /locus_tag="ACGI59_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HFG9738183.1"
                     /translation="MKCTNFTFKGEEGLDIYTYKWEDENIKKPKAVIQIAHGMAETAQ
                     RYETFAKVLTKNGYIVYINDHRGHGKTAKIIENVGHLAEKEGFRCLVEDMYTLTNIIK
                     KENEDLPIYLFGHSMGSFASQRYIMDYSNNLSGLILCGSNGKQGIILNLAHLIINREI
                     KKYGRRSKSNKINNLIFGGEIIRRNEKTKFDWLSRDKEQVEKYINDPFCGVVCSCGFF
                     YDLVQGLKEIEDKENLKKVPLDIPIYIISGDKDPIGKNGKGVLRLRDRYIKLGVKDVT
                     CKLYKDGRHELLNEINREEVFEDIICWLNNKIEIL"
     gene            15249..16463
                     /locus_tag="ACGI59_000371"
     CDS             15249..16463
                     /locus_tag="ACGI59_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFG9738184.1"
                     /translation="MQIKIGKVIQCLRKERNLTQEQLAKFIGVSTPAVSKWESGNSYP
                     DIELLPLLADFFNVSIDKLLNYKIDLSEEEVMKIYKELESGFARIEIDLSTEEPKEEF
                     RQDLESVKKLSNMYIEKYPKSYLLKLRICSLYQMYSYKFGKSELNDRVKETTNILEDI
                     VRNTDDIQIKETALIILSNAYCMLEDYEKAELYLNMIHKSIGDTSVNLAMIYLKQNRL
                     EEAEILLQNKLFSNVFNISMDCKGIINVYKNQYKELKKKLENRNFNKNAIEREMEYIK
                     NKLLGYANLSLEIKKMLSEDKGAFFSMYMDYMELSLIFLFFNMKEEAKKALYSLKEIL
                     EKYPIHESLDVSQMRFFDKVESKNLYTFNIYTNLLIVLNDDSYNELREEPIFKDVIEK
                     ILDMEKMLKNKE"
     gene            complement(16883..17404)
                     /locus_tag="ACGI59_000372"
     CDS             complement(16883..17404)
                     /locus_tag="ACGI59_000372"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFG9738185.1"
                     /translation="MKFERLRTSENKLYYKAMELYKISFPFHKQRKSSLQAEILKNKE
                     YQFNLIYDKNQFVGIILCWETDNFIYVEHFCIFPEIRNNRYGQRALELLNKKGKTIIL
                     EIDLPIDEISIYRKAFYERVKYKTNNYEHIHPPYHKKFNGHNLIVMSYPEKLSKMEYD
                     KFNHYLRNNVMSF"
     gene            17760..18557
                     /locus_tag="ACGI59_000373"
     CDS             17760..18557
                     /locus_tag="ACGI59_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HFG9738186.1"
                     /translation="MEKREKRIIISSLLSVSILMGLASIYSILNKEDISLTVKGQEQK
                     VSSFKKTVEELLDEQGVKYNSEDKINPSLDTELKDDMKIKVVKVTKSKKEEIEKIPFD
                     TKHVNDSNLLKGKSKVYQEGQEGEKKLVYNLTYHDGKLVKKVLSKEVISKEPTTKIIK
                     YGTKEKVLIASRGANIRGGKHMKVVATAYAGDTITSTGTTPRWGVIAVDPRVIPYGTK
                     VYIPKLGMTFVAEDCGGAIKGNRIDIFMNSEGKASNWGRKSIDIYLH"
     gene            18667..19257
                     /locus_tag="ACGI59_000374"
     CDS             18667..19257
                     /locus_tag="ACGI59_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFG9738187.1"
                     /translation="MKIVCLGDSLTYGFGVSRSNSWTNIVNKETRLEIVNKGINGDTT
                     SGMLARFNEDVVKNSPDIVFIMGGTNDFIAGAGNEIINSNIMAMVHQSYSKNIIPIIG
                     IPLKPDIPNVREDWSCFTDFNIVSQNLESYSYWIKKFCMTFNTNFVDFYSEYNKNMEL
                     EGYKKLYFDGLHPTKEGHRIMADIFINSINKYIEEL"
     gene            19323..21020
                     /locus_tag="ACGI59_000375"
     CDS             19323..21020
                     /locus_tag="ACGI59_000375"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HFG9738188.1"
                     /translation="MDYTKTYEEWIKGSYFDEDTKLELENIKNNEKEIEDRFYKDLEF
                     GTAGLRGIIEAGTNRINKYTVRRATFGLANYILENTTKEETSRGVVIAHDNRHKSRQF
                     CIESANTLAACGIKAYIFDSLRTTPELSFAVRSLNAIAGIVITASHNPPEYNGYKVYW
                     EDGAQVMPEIANAITEKVNSIHDYSTIPTLTEANKNLVVLLDESQDTKFIEAVKSQII
                     RKDLVKNVGKSFKIVYTPLCGTGNVPIRRALKEVGFENIIVVPEEENPDPNFAGLDYP
                     NPEEKKALNRGILLAKEKGADLVIATDPDCDRVGVAVKTTTGEYALLTGNQIGGMLTH
                     YIIEGLKENNKLKENPTIIKTIVTSEFGADIAKANNVDVLNVLTGFKFIGEKIKLFEQ
                     NKNRSYVFGYEESYGYLVGTHARDKDGVVSSLLISEMAAFYYSKGMSLYDGLIELYKK
                     YGFFKEQTISLTLKGIEGVEKIKEIISYFRENQIDYINSIKVVDKKDYKNGIDNLPKS
                     DVLKYFLEDESWVAIRPSGTEPKLKFYIAVKGASDIEADKKIQGLKKYIDDMVEKLK"
     gene            21234..22073
                     /locus_tag="ACGI59_000376"
     CDS             21234..22073
                     /locus_tag="ACGI59_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HFG9738189.1"
                     /translation="MFGIDKKLIEYIIAKSNYSELSSKSAEVSFFLMLSIFPFLIFTI
                     SSIAYIPILHLNKYIALFRNMMPEGAFAVLSSIIVSAIDNRNLKFLAVSFVLTMWTFS
                     RAVKALIKGMNRAYKVKETRSFFKILSISFLFTIMLLVLIFLSMIFLVYGEKIGYFIF
                     NLVGLDEIFIKIWDILRYTVGIITIIVIFTLLYKYTPNKKLTIKESAPGAIFATFAWF
                     LVSFLYSYYTNYYANYEVIYGSIAEIIVLMTWMYFSSWSIVIGYEVNSRLYFRKIRHE
                     MLK"
     gene            22590..24446
                     /locus_tag="ACGI59_000377"
     CDS             22590..24446
                     /locus_tag="ACGI59_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFG9738190.1"
                     /translation="MDTHNKYVNFIKNIPVPFLYCRIVKRQEDIEYRVEYISKGMGKV
                     LQLEEGICDKNILDVLPVFKSKKYFKELFSNEVDCIKRYIPTLKNWINIKKQIIGDSY
                     IILYFGKIVFDYRQIIDSFDKKEKVAYIKDEEGTYIDCSENLIPILNNNIKTTKDIFG
                     KNDIEVWGENTGKLFRDDYREGVSSKKRFLQNLFEYEETFFMVEKYFLYDEDELLGTI
                     GIVDNIIYSGYSNRNYNSKDLMKMIEHSIPENMFYKDVYGNYIGFNSGFLNLACMNKE
                     ELLGKNSYKISEEEALIDKIFESDKGVVENKKVVTFELNISMNDENKCIEITKRPFFD
                     SYGSVIGIIGTARDISRRKRLEEEMDKTRMEFFANLSHELRTPINLISSSLQVIEKKE
                     ADLIESNDTLKRNLGIIKQNGNRILRLVNNVIDFTKMQSGYLDFKPEESDIIAFIEEI
                     CMSVADFASQNNIQLTFDTEIEEFSMLFDSEKLERIILNLLSNGIKYNKKDGKINIFL
                     YVKDNVFNMKISDSGIGIPKEKIDKIFNRFEQIDNELSYRVKGSGIGLSLVKSLVELH
                     EGSISLKSQLGIGSEFIVSLPVRSKNNIEKYNHKREISNELSKKLEIEFSDL"
     misc_binding    24529..24769
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    24835..25070
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            25155..26372
                     /locus_tag="ACGI59_000378"
     CDS             25155..26372
                     /locus_tag="ACGI59_000378"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HFG9738191.1"
                     /translation="MENKVKIGVLGYGVVGSGLIDIIDNNKEKRNIEIVGILVNNLEK
                     HKDKKYSNIITNNIDDIFNKDIDILVEVMGGLEPSLSYIKKALNNKIHVVTANKDLLA
                     ECGDELAKLASENKVSIKFEASVAGGIPVLKPIIESLEGNNIDSINAILNGTTNFILS
                     KMYDENLSYDMALRQAQELGFAEANPESDVLGYDAARKLSILSTLAYDNRVYWKDVYL
                     EGITDIDEKDIEYAKKLNCKIKLIGQSKYENDKVSAFVRPVLVEKDNILARIDNEFNA
                     VIVNGDSVGEVSFVGKGAGSLATGSAVYSDVIDIIDNRVSSIDSFTKDKIQVNKIVRE
                     KCGALLRFKKCNKDEILNIVENCLVKFDILNDDDELAIMVYADSEYEINNSLCLIKDK
                     GYCEKMNKMLKIS"
     gene            26489..26971
                     /gene="cdeM"
                     /locus_tag="ACGI59_000379"
     CDS             26489..26971
                     /gene="cdeM"
                     /locus_tag="ACGI59_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium morphogenetic protein CdeM"
                     /protein_id="HFG9738192.1"
                     /translation="MENKKCYSEDWYERGESTAKWFQNDREEYEREAYDEDRERRGSN
                     CGCSDSGENRPRNCERFRREAEIREREAREAFCESSEKKKEALAYECEARKLWEEAEK
                     YWDEYSKYNYKGIEYLAEAARLFDEGMECEARRNGNNGGNNNNCCHKCHKCNCNCCRK
                     "
     gene            27235..28500
                     /gene="hisD"
                     /locus_tag="ACGI59_000380"
     CDS             27235..28500
                     /gene="hisD"
                     /locus_tag="ACGI59_000380"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HFG9738193.1"
                     /translation="MKSIKTSSMKEKVDEKILREQVSSIIEDIRNNKDVALKKYNEKF
                     DRNTRDEFRITKEEIKEAYKHVDDEFINNLKIAAKNIREFAKAQKLSFENPFEKEIYP
                     GVILGQTNIPIESCLAYVPGGQYPLFSTALMLIIPAKVAGVKRIVACSPTMKNTEKIN
                     PKTLVAMDIAGADEIYATGGVQAIAAFTYGTEKINPVDIIVGPGNKFVTEAKRQCYGQ
                     VGIDFVAGPSEVLIIADETSNPVYIAADLLAQCEHDLNARGILLTNSLEVAQKVEKNI
                     ESMLKDLPTKDIAYSSWENNGEIILVDDMEEAIKISNFYAPEHLEVAVNEYNDICDRL
                     TNYGSLFIGNLSAEVFGDYVSGTNHTLPTLKASRYTGGVWVGTFIKTCTKQIFNEKAI
                     QSLAPVAEKLAKEEGLYAHAKAAEVRFKK"
     gene            complement(28703..29455)
                     /locus_tag="ACGI59_000381"
     CDS             complement(28703..29455)
                     /locus_tag="ACGI59_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFG9738194.1"
                     /translation="MSYFQYQGNSCFYKEYGQGKPIIFLHGNTGSSNMFKALVPLYAE
                     NFRCILIDFLGNGQSDRVSQFSPDIWHDEALQTIALIEHLNCGKVGLIGTSGGAWAAV
                     NTGLERPDLVEAIIADSFDGRTLNDNFIDNLITGREKSKQDIQARKFYEWCHGKDWEN
                     VVNLDTKALLQCANEKRPLFHKELCKLEMPILFTGSKEDEMCRHNLEEEYKQMATFIS
                     KASIYIFSQGGHPAILTNADEFAQLAKVFFKY"
     gene            complement(29573..29842)
                     /locus_tag="ACGI59_000382"
     CDS             complement(29573..29842)
                     /locus_tag="ACGI59_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3324 family protein"
                     /protein_id="HFG9738195.1"
                     /translation="MSYQNSIDLLPKELIEQVQEYIDGKVIYIPKKQEHKKHWGENTN
                     TKQVLASRNSQICINFQNGMSIKQLSEKYFLTEKSIQRILKQQNL"
     gene            30317..31411
                     /locus_tag="ACGI59_000383"
     CDS             30317..31411
                     /locus_tag="ACGI59_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFG9738196.1"
                     /translation="MKNIVGKPQTIKKVNEDLIKNIIKNEGPITKPEISSITELSLAT
                     VNKIVEQLALKNEVKVSGLSESTGGRRAQLFEINANLEHIIALYYYRNCCIGVVANLL
                     GEIIYQEEFNIRMDTYENVNQDTFFVIDTLIKKEKGNNIRAIGIGVPGVVKKGVVKNI
                     PTIKSWEGINLENLIKSKYNTKVFIENDTNITTIGIYQKQYKDKYKNMALMYLEDGIG
                     SGIVINGELYLGSTNFAGELSYLNIEFGKYNSNNLNLEDYIMSLRKRYLESKDNKFKK
                     EILSIISNFIRTLVCVLNPQAMIIQCSILTKSDMEFIEKDVSNNVGEENFPKLIKVDS
                     LREGSINGVISMCLKETDYQYSLSNKKGGY"
     gene            31413..32363
                     /locus_tag="ACGI59_000384"
     CDS             31413..32363
                     /locus_tag="ACGI59_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HFG9738197.1"
                     /translation="MKYLVSIDGGGTKTKFCVSDLDGNILKEHTTGSTNYKSVGIKKT
                     YENINNGFKKILKDLYIDYDDIEYTVFGISGCDSPNDYKIIMDEILKIGINKEKIYLA
                     NDAVLAFYAQADSPGLVIVAGTGSIILGIKEDGEIYRVGGWGYNFSDLGSGYDIGRKL
                     LKKVLLYCDECHEYSDLFSCVLDFFGANSFEQLAYMITDINDNVEIANLASLVIDCAE
                     QGDKLAIEILRESSTELSKLAQVILRKISNPMKMDKSEINIVLSGGTLNKTVYAQMLM
                     NNLKTENTDKNLKFISQENQPVYGGIRLAMALANRRVKHG"
     gene            32356..33540
                     /locus_tag="ACGI59_000385"
     CDS             32356..33540
                     /locus_tag="ACGI59_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9738198.1"
                     /translation="MDRKKVIVQIFTGGFKAKEIKFNDIKCKLLELLDCIDIEKVIMG
                     WSVDEELYVETKKLLNKYNVELYLWLPCFSEIGLLEKSNLLIDYMGKEVKGYALQEDE
                     NFEFYCPNNKENIKNIKKIYSTYFSDIKFDGIFLDKIRYGSFSNKLNGVFNCFCKDCL
                     NIYKKNNIDKESLMREMNNVSKGVIDNEDTPFGITEYINGKYKFKNKIWEDFFETKNQ
                     NIYKALLEISSYFKSKGLKIGIDAFSPFISYFTGQDLEKLQEVADFIKPMMYRTTKAP
                     AGLPFEFERFIDESIHKNKEIGKRNFLDILGIKEYEGDVYPIEFVLEEIDFMTRNLNK
                     DIYVGVEINKKEIISPVTPKYISENISNINKTTVKGYVLSWDLLSAPKENIDEIIKWF
                     RN"
     gene            33553..35049
                     /locus_tag="ACGI59_000386"
     CDS             33553..35049
                     /locus_tag="ACGI59_000386"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFG9738199.1"
                     /translation="MSKILEIQQIYKSFGKVDVLKGINFELEKGEVRALLGANGAGKS
                     TLIKILGGVHSQTKGKVILTGNEIMFKNPHHSKENGISIIYQELSLIPTLTVLENIFL
                     GREILKGSVFLNKKDMLEEYMKICEDFDFNIDPHIVVSNLSVANQQMVEIMKAVSCNS
                     EIIIMDEPTTSLTNNEKDGLFKIISRLKSKGKTIVYISHILEEVFKVCDSASIMKNGI
                     MIGTYDVEDLTKLKITQLMTGNDNHKLIEKKDYTFAKYEEPPVLEVKNISWSNKIKNV
                     SFKVYRGEVVGLAGLVGSRRTEIINLIFGIDNKDSGDIFINGKKVDITSPKVAIKNKI
                     GLIPEDRKNLGLILEQAIYTNSSSVQIDKFKKFLFIDRNKEIEFSKKGVKELGIKVSD
                     VKQEVKELSGGNQQKVVVSKWLEKDLDLIIYDEPTKGIDIAAKEDIFNTIKEFSSKGI
                     GVIFISSDLEEVIRVSDRILVTREGSIVDDLKNENIQVQDIMNSIFNV"
     gene            35072..36022
                     /locus_tag="ACGI59_000387"
     CDS             35072..36022
                     /locus_tag="ACGI59_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9738200.1"
                     /translation="MKKAFEKLNLRDYGVLLGFLALCIAISIATPSFLGKQNILNLLR
                     QSSIIGIISAGMTFVIISGNFDISVGAVAALAGAITMKFATGGTNLFASMFLGVAICA
                     VIGLINGIVVAKINVPSLIATMAMVTIVRGLLLMLTGGYPITENIPILDYIGNGYLFN
                     IPIPVVIFFIVVLASFIVLNKTKFGRYVYSVGGNQEASKLNGINVDSHKVKVFIINAV
                     LAGIAGIVLTGRLGTATAIAGEGYDMDAIASVVIGGTSVAGGSGSVLKTVIGVLLMSV
                     INNSFNLLGIDVYFQYIFKGLIILAAVGFDSYSKKKLASR"
     gene            36043..37005
                     /locus_tag="ACGI59_000388"
     CDS             36043..37005
                     /locus_tag="ACGI59_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HFG9738201.1"
                     /translation="MKKLMKHLALLLSLVMIFGLVGCSNGGDKEKNGEKKIAVLLPGS
                     TGYFVATKQGIDAKSKELGVSVEYADAQWDASKQLSQAEDFMAKGVDMIALCGVDSAV
                     SERIVKAANDSDVPIVAFTNSIGSNPTGEFKGLVTYIGQNEEETGALTGKIAKNLLGE
                     TGGKAVLIEGVPGTTPQVLRKKGLEKELKDSNIEIVYNQTSNWEKEQALKVTEDLIQK
                     KTDFNVIICQDDNSATGAGQALKDAGLKDKVKVIGLGGSKDGLKAITDGLIDGTTYMS
                     AVEEGGLVIEKASKFLKGEKIEPVTQIKQVEVNKDNISEFKGEW"
     gene            37377..38009
                     /locus_tag="ACGI59_000389"
     CDS             37377..38009
                     /locus_tag="ACGI59_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFG9738202.1"
                     /translation="MARRLFLERIGLIFLGSAILAFGVYNFYYLNNITEGGVLGILLL
                     LKNLFNIQPAIANVVIDGLLLLVGYKFFGKKFLIYSIVASITFSVLYDLFEAIGPLVP
                     QSQNMLLSTILAGVTVGTGVGIVVKAGCASGGDDALALVISKTTSLNIGQVYLATDVI
                     VLLLSLFYLSAFDIFYSLIAVTISGKVIDFIYYHGKSLDMDISNDIVPEC"
     gene            38435..38512
                     /gene="kdpF"
                     /locus_tag="ACGI59_000390"
     CDS             38435..38512
                     /gene="kdpF"
                     /locus_tag="ACGI59_000390"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA];
                     GO_process: GO:0043462 - regulation of ATP-dependent
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HFG9738203.1"
                     /translation="MWFLAVVIIMLIIYLVYALINPEKF"
     gene            38559..40235
                     /gene="kdpA"
                     /locus_tag="ACGI59_000391"
     CDS             38559..40235
                     /gene="kdpA"
                     /locus_tag="ACGI59_000391"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HFG9738204.1"
                     /translation="MVQIVIVLAIFMILVIPMGKYLYHIATNQKTFGDRLFDKVDNFI
                     YKVCSIDKKKEMNWKQYALALLFTNAVMVFIGYIILRTQSMHIFNPSGIKSMEQGLSF
                     NTIISFMTNTNLQHYSGESGLSYFSQMTVIIYMMFTSAATGYAAAMAFVRGLVGKKKT
                     LGNFYVDLIRITTRVLLPGALIIGLILVTQGVPQTFAGTETVTTIEGKLQDIARGPVA
                     ALESIKHLGTNGGGFFGSNSSHPFENPTIISNIVEILSMMILPGACVVAFGHMIKNKK
                     QGWVVFGAMSIIFLIGLVVCFKAESAGNPILSQLGLNQSMGSMEGKEVRFGIAQSSLF
                     TTVTTSFTTGTVNNMHDTLTPLGGLVPLLNMMLNVVFGGKGVGLMNMLMYAIIAVFLC
                     GLMVGRTPEFLTKKIEGKEMKLIALLIILHPLLILMFSGLSVAIPAGLEGISNPGFHG
                     LSQVLYEFASSAANNGSGFEGLGDNTMFWNITTGVVMFFGRYVSIIVLLAISSLLASK
                     KAVNESIGTLRTDNFTFTIVLVLVVLIVGALTFFPALALGPISEHLVLWH"
     gene            40260..42317
                     /gene="kdpB"
                     /locus_tag="ACGI59_000392"
     CDS             40260..42317
                     /gene="kdpB"
                     /locus_tag="ACGI59_000392"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HFG9738205.1"
                     /translation="METKKTKFITKDIFKTSIIEAFKKLNPKYMMKNPVMFVVEIGFI
                     VTLILTIVPSLFGDEGNNLRAYNGIVTIILFITVLFANFAEAVAEGRGKAQADTLKKT
                     RKDTVARVIDTLGNEKMISASELKLGDIVLVNAGEVIPNDGEVIEGIASVDESAITGE
                     SAPVMRESGGDFASVTGGTTVVSDWLKIRITTKPGESFLDKMIALVEGASRQKTPNEI
                     ALNTLLVGLTLIFMVVLISLYPMANYAGVKIPISTMIALLVCLIPTTIGGLLSAIGIA
                     GMDRVTRFNVIAMSGKAVEACGDVDTMILDKTGTITYGNRLASEFIAVGNADSKDLIN
                     YSVMCSLKDDTPEGKSIVDLGMKLGSTGKTKEAEEAEFVEFSAQTRMSGINLADGRAI
                     RKGAYDSIIKRVKESGGTVPEDLEENVNRVAKLGGTPLVVCVDNEIYGIIYLKDTVKS
                     GLVERFARLREIGIKTIMCTGDNPLTAATIAREAGVDGFIAECKPEDKIDAIKKEQLE
                     GKIVAMTGDGTNDAPALAQADVGLAMNSGTTSAKEAANMVDLDSDPTKILEVVEIGKQ
                     LLITRGALTTFSIANDVAKYFAIIPAIFTLAIPQMEILNVMKLSTSFSAILSALIFNA
                     IIIPCLIPLAMKGVKYRPMKSQAMLMRNMLIFGLGGVIVPFLGIKLIDLLITPLVMVL
                     NLG"
     gene            42336..42962
                     /locus_tag="ACGI59_000393"
     CDS             42336..42962
                     /locus_tag="ACGI59_000393"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HFG9738206.1"
                     /translation="MKTLKPALLVSIVLLVVCGLVYPLVLTGVSQVAFKDKANGSMIE
                     VNGVKVGSELIGQSFTDARFFKGRVSSVNYNTYTKEDLVPDKDGNTSYGGVSSGSFNY
                     GATNPELHDRVQKDIEKFLKDNPTVKREDIPTDLLTASGSGLDPNISPASAKIQIPAI
                     AKASGISESKLQKIVDDNTSKKLFGVLGEDRVNVLKVNVEVAKILGLI"
     gene            43199..44107
                     /gene="cysK"
                     /locus_tag="ACGI59_000394"
     CDS             43199..44107
                     /gene="cysK"
                     /locus_tag="ACGI59_000394"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFG9738207.1"
                     /translation="MLYNNALELIGNTPVVRLNNLGCTNGCSNIYVKLEKVNPAGSIK
                     DRAVYEMLEGLEQRGELKKGDVLVEATSGNTGIALSMIGKLKGYEVVIVMPETMSVER
                     RDLMKAYGANLVLTDGKAGMAGSIEKANELLKENPNYKSLKQFENKDNPNAHYKTTAV
                     EIMNDVKDLDIFVCGVGTGGTLIGTARYLKEQNPNIRVIALEPKKSPAISENKTGPHK
                     IQGIGANFVPENYDSSVVDEVILVDDEDAFDTVKLLAAKEGILVGISSGANIFGAIEM
                     AKKYPDKKIVTVAPDGVDKYMSMGIF"
     gene            44134..44724
                     /gene="epsC"
                     /locus_tag="ACGI59_000395"
     CDS             44134..44724
                     /gene="epsC"
                     /locus_tag="ACGI59_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HFG9738208.1"
                     /translation="MFKKINKDIEYIMKNDPAARSKIEVFLLYPSVHAMIMHRMAHAL
                     YKKKKLFTARLISQISRFMTGIEIHPGAKMGEGILIDHGMGVVIGETAEVGNRVTIYQ
                     GATLGATGKDTGKRHPTVGDDVLIGAGTKILGPLNIGSNSKIGANSVVVKDVPNGATV
                     VGIPAKIVKIRNLEPVKKNKKEVSYEYDELDNVYYI"
     gene            44884..45072
                     /locus_tag="ACGI59_000396"
     CDS             44884..45072
                     /locus_tag="ACGI59_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HFG9738209.1"
                     /translation="MKANVNQDTCIGCGLCPSICPEVFDMKDDGKSHVIVDEVPSDAE
                     ESAAEARESCPVDAIDVH"
     gene            complement(45126..45995)
                     /locus_tag="ACGI59_000397"
     CDS             complement(45126..45995)
                     /locus_tag="ACGI59_000397"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HFG9738210.1"
                     /translation="MKLNRNDSCWCGSTKKYKKCHMDMDNKIDSFAKLGHIVPTHDII
                     KNESQIEEIKKSAVINTKILDYISDKIEIGMSTEDINKLVHDFTLQNGAIPAPLDYNG
                     FPKSVCTSVNNEVCHGIPSKDIILKDGDIINVDVSTIFNGYYSDASRMFMIGNVNENA
                     KKLVQVTKECVQKGLEVAKPWGFLGDIGDAINSYAKKNGYSVVREIGGHGVGLSFHED
                     PFVSYVSKKGTEMLLVPGMIFTIEPMVNEGTHEIFVDEENNWTIYTKDGKLSAQWECM
                     VLVTETGVEILTE"
     gene            complement(46192..47436)
                     /locus_tag="ACGI59_000398"
     CDS             complement(46192..47436)
                     /locus_tag="ACGI59_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9738211.1"
                     /translation="MENNLNTAKRNNTELLQNDSLKVNLQIGVDFTLLSKSLKAFYKK
                     EKDFYCIALAPCNVSSEKANRKISIYDMIKEINILIESITDKNELLSEELFTNNLSEF
                     YDESLDEIYIDLKQAYLYIKKNTDGLNIEDSIEYVFDVDISNDIKAKENPLINFNSLS
                     LVVWSGETKHILQQMNILDLEDIHSYSIENNTNITIYENNTLQYKFDEESENIKDSSI
                     DIIEQMLILPDDLNYDKDEVENMKNRLAKINIKYLQTLKEKDIKIKLINSNLTDEPEF
                     SDLKYQLPPCWVRSGKTWKDVPGIYRNNSIVAKIGYSNPSYANVHSSKNLELHETAHA
                     IDKNVLNKKSNSEEFMEVFAQERYKLYDPKQVAHAYISKFIEEFFAESFVHYYLDEDS
                     KNTLKENCPLTYDFLEKLELNY"
     gene            47727..48038
                     /locus_tag="ACGI59_000399"
     CDS             47727..48038
                     /locus_tag="ACGI59_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFG9738212.1"
                     /translation="MYFTYIIRCKDDSLYTGYTSNIVRRMNEHKLGINSKYTRAKGFE
                     KLEVYFVTNTKSNAMKLEYYIKKLTRNKKLSIIKNPSILINLIDNKEDYIIGKEIEQL
                     T"
     regulatory      48131..48223
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            48323..49117
                     /gene="thiD"
                     /locus_tag="ACGI59_000400"
     CDS             48323..49117
                     /gene="thiD"
                     /locus_tag="ACGI59_000400"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFG9738213.1"
                     /translation="MSNYKIPTLTIAGSDSSGGAGIQADLKTFSAIGTYGMSVITAIT
                     AQNTKGVFAVEDLNKKIIKKQIEAVFEDIPPRAVKIGMVSSPEIILEIVENLKKYNPK
                     YLVVDPVMISKSGYYLLKPEAKENLIKYLIPLAYIITPNIPEAEEITGIKIHNVDDMK
                     RVGEEILQLGPKFVLMKGGHLDGEAVDILVGKNIFKVYKSERIDKKNTHGTGCTLSSA
                     ITSYLALGYEITEAVNLSKIYITEAIKRSFDIGHGVGPVHHFYKFE"
     gene            49141..49929
                     /gene="thiM"
                     /locus_tag="ACGI59_000401"
     CDS             49141..49929
                     /gene="thiM"
                     /locus_tag="ACGI59_000401"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HFG9738214.1"
                     /translation="MYNLIKDVKKLNPLVIHYTNNVTINDCANVTLAVGASPLMSFSY
                     EEVEEMVSVANSVVINIGTMNSNMLDLFLLAGKAANKYNKPVVLDPVGVFASKARAEL
                     TSRLLNEVKFSVVKGNVSEIKFIGGFNVRGKGVDSFDEEEDSTEIIRKIAEKLECVVV
                     ATGKIDIITNGKGTYKINNGTDKLKGITGTGCMTASLIASFMAVTENILEAATMGVLT
                     MSLSGELANLNNPPIGTFKENLMNAIYQMDIDALSKNSNIEFLN"
     gene            49952..50584
                     /gene="thiE"
                     /locus_tag="ACGI59_000402"
     CDS             49952..50584
                     /gene="thiE"
                     /locus_tag="ACGI59_000402"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HFG9738215.1"
                     /translation="MIDKESLKKCLKLYLVTDSEMLKGRDFYKCLEDAISSGITTVQL
                     REKNASGREFLRKAMKLREITKRYGVKFIINDRVDIALICDADGVHVGQSDIDVREVR
                     KLIGNNKILGVSARTLEEAICAKNDGADYLGVGSIFTTSTKLDAKSASFETVKEIKEK
                     VDMPFVLIGGINLDNIDKLKCLESDGYAIISAILKAEDISKEVEKWTLKI"
     gene            50723..51025
                     /locus_tag="ACGI59_000403"
     CDS             50723..51025
                     /locus_tag="ACGI59_000403"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFG9738216.1"
                     /translation="MIRILLVCVGGMSSTLLVNKMEKDAKKRNIDCKIWAVGEGDIKS
                     ELDNFDILLLGPQLRFMLDDVKSIVGDRAPVSIIDMVNYGTCNGHAVLNSVLEILK"
     gene            complement(51091..51840)
                     /locus_tag="ACGI59_000404"
     CDS             complement(51091..51840)
                     /locus_tag="ACGI59_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9738217.1"
                     /translation="MLNILWRSMKWRFKNPISFVVTILQPFLWLVLYSSIANQTMNNI
                     NINNYTAFILPGIIVLVVFSSCSSGGIINFIMKNSGSFYRVLIAPISRYSIVLGQLLE
                     AILVSFIEVTILCIVSIFFSVRIESGIGGILLMIVLIFMTAFFLSSLAYSISLLLPNE
                     IVYETIMTAIVLPIFFLSSALFPIESLSGGLKVAVMLNPFTHVINALRSLIFGETILI
                     GDIMPVILLFLILCCSSFSLAMWRLKKEMVS"
     gene            complement(51834..52796)
                     /locus_tag="ACGI59_000405"
     CDS             complement(51834..52796)
                     /locus_tag="ACGI59_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFG9738218.1"
                     /translation="MLAIEIDNLVKEYKNGVKALNGLSFNVNAGEIFSLLGPNGAGKS
                     SLINILTTFYKPTSGNVTMFGKDLVDNPSWIRTQIACVAQQISIDEHLSLMENMVFQS
                     KMYKVEPQIAKQRIDSLIDKFDLSSYLKYPTSSYSGGVKRRLDIAMNMVSSPKILFLD
                     EPTVGMDVDSRKSMWDMLLKIRDEYGTTIFLTTHYLEEAEQLSDNICIMKNGKDLAQG
                     TPSSLRSYIRQNILRITFHNTEDIKKYKDSIKSTGLVKFMSVRENSIFISVNDSRTAF
                     TLINKWLLEHDIEFDAIEIVEPSLEDVFLALTSSKKSLKEEWEC"
     gene            complement(52800..53258)
                     /locus_tag="ACGI59_000406"
     CDS             complement(52800..53258)
                     /locus_tag="ACGI59_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFG9738219.1"
                     /translation="MNCKIEIKNIESVTVATMRYNGPMTEATKYFPNVFKAIKGKSNG
                     APFFCYYDVDQKTGVAEMELCVPTAETPNRMGITTKEFPKTKALFFTHTGSYSNLPKT
                     YEMIFKYIHENNIKIQTPWREVFIKGPGMLIKGNPDNYITEIIFPLKEEE"
     gene            53614..53970
                     /locus_tag="ACGI59_000407"
     CDS             53614..53970
                     /locus_tag="ACGI59_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFG9738220.1"
                     /translation="MNFELDNTTPIYLQIVKYIKRQIVTGELKPGETIPSRREMALNL
                     KVNLNTVQRAYKEMGDMNIINTFKNYQSSVTVDENILKNLKLELINESLSVFIEDMKA
                     INVSKEEVLKIIEDKY"
     gene            54042..54758
                     /locus_tag="ACGI59_000408"
     CDS             54042..54758
                     /locus_tag="ACGI59_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9738221.1"
                     /translation="MIEIKNVSKTYKRMQGLKIRKIEALKNVSFNIEKGKITALLGIN
                     GVGKSTMLKAIAGLINIDSGEIRIDGEKINEKVYNKLAFVPDVQSHFSNTTIKETFEF
                     MEIFYSKWNKEKSKEMMDIFKLDEDEIIDNLSKGNIARVKLILGFCQDPEYILLDEPF
                     TGIDLFKREEFIGVIAQYMEENQAIIITTHEIVEIESLVDEVVILDEGQIITSFNAEE
                     LREREGKSILDKMREVYKNE"
     gene            54751..55575
                     /locus_tag="ACGI59_000409"
     CDS             54751..55575
                     /locus_tag="ACGI59_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9738222.1"
                     /translation="MNKLLYLINVDLRRSNKFYIAYISFFSLITLSLNLFEINKFKNS
                     KFIMNLAIEDFGGIFYGLGILSNLGFIQSILFFGILGLFIYYIFMWKREFFSNNQSIY
                     TLMMLPQNKFKICISKSIALITMIYGFLISQTATLFICKYIFNFIFRNMPIINMNFAK
                     DLYYLNLKSIPIDFISFMAIHVFLLLIAISIVNCCILFIFSYINKFYVILITLLIIAG
                     LYFSTIYINIITLFRKYYNLIGFQFNNLVAGLLSFLIIMFILNGISYLLIKKKVSL"
     gene            55587..56786
                     /locus_tag="ACGI59_000410"
     CDS             55587..56786
                     /locus_tag="ACGI59_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9738223.1"
                     /translation="MKLKSKISIFIIFLTLAIYFSIGLLNNEDNLKIKTIKGDSNELD
                     HININFGYALNGYINSYYTINGSNKTKKTELIKLQKDSKNTNNLIDVSSGSDLIAHVG
                     FEMNEESKDINFVIDEKKEDLEKGVFPSEGSDKEIEYEKLDMNNFTRTRYKVDKSTDD
                     YYAKILASKRYKKYLYVSVLYATSDSSENNKEYSSTIDIIKINPENKKIKTIKSINLS
                     NYFSEKYQNIENIGVSYASKYEDKMYILVNVLNTNIGDNSNSKFHLLEYNLEENKESI
                     MELKTDSKMALAKAKLEANKLYMVMSNDYDNSSITELNLLTYNILDKSLDEDKYVLDN
                     REKRGIRSIEIDSNKIVAYTSRLNSILSWDDKFMLYIINKNSKEIVYEGELQFNSSNS
                     MLEFEIK"
     gene            56795..57607
                     /locus_tag="ACGI59_000411"
     CDS             56795..57607
                     /locus_tag="ACGI59_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9738224.1"
                     /translation="MKDLIRLFEFELKRNIKNYIFIIICCCSFVILNIVKNLNDYNYI
                     IENAVKSEQLTKIGNTIETVNVTGFSSFRNIMGSTESWFMFGIIACICYAFFIWYRDF
                     NGRSKSIYTLIMLPKNRINIYISKLLNILFLVYSYTVVLTISLFIASKLLPRHMLGNV
                     TNYGFVQETIYELKMLLPYSFEILFVEYIFLLIGFVSVVFTSILINKSIYKVSTLISF
                     LFLVIEILVFIISIEFIIPYEYSDVFTVLYSSINIFVCSLISNKLLKQKIDF"
     gene            57618..58886
                     /locus_tag="ACGI59_000412"
     CDS             57618..58886
                     /locus_tag="ACGI59_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9738225.1"
                     /translation="MKAKNIGKYTICFIIVCFLMITMTLILSYFNKDKLSVKTLKGNV
                     KDIGRVSVVYTPNISKFSYEEVIISKNGVRNNTNPKSRIRNDYLNIDKKYSDFVNDKG
                     KEFIYKDDLNIGEAYTSVKSDLVQGMNALNDCIKVSNKNLKSGVEENFDINLNKSDFK
                     ENSWKTLFNKIYKGNLYMVISETNISGSKVNNICILKIDIKNKSYKLLNRFNLGLQNG
                     EQQNINEIKFSIGSKLYLEIVNNKSKNGYIEGHDSYSNFIIYDIEDNTFNISNKFMDN
                     LNKEKNANPKEIGLLSKTKLDYMVKNNKLNLIVTDMSNSITNLVYDINSNEIKLEDYE
                     ILDLKVDYNEASSGVRKVKLLDDKIYSISGIKNSISGELKGFKGIIDVVGTKPVKFQV
                     FDMKLKKNIYEAELINGDISLQEKLFFVAN"
BASE COUNT        23425 a         6081 c        10190 g        19337 t
ORIGIN      
        1 ctatataata ctcaattttc aacattctat acaattatac agaaattata cagcataata
       61 atcaccgaaa taacaaattt taaaaattaa gcaaaacttt ttgtcgaagt tcaacgtcta
      121 tatatatgta gtgtaaattt ttgggataaa atactacaaa aatataaatg taaaaatata
      181 gtaatattgg aggtcgaacg atgagaaata aaaagaaaaa aataaataga aagaagttgt
      241 atttgctttt aagtgctatt gtagtgtgtc tggcttttat agtagttggt gttcgcgtca
      301 gctctttaaa tatgaaggaa aatgaaatta gtagaaatgc aaaactgtca agttctacta
      361 ttacagatat agtatctaat acatcaatag aagttagtaa agggccaagt aaaagttctg
      421 gaaaaatagc atacataact atagatgatg gtccatctaa atttactgac cagatgataa
      481 aaactttaaa taaatacaat gttaaagcaa ctttttttat gatagatggg aatatgaagg
      541 aatatccaca gcaagttaaa aatataatta aaaatgggaa cacagcaggt tttcatagtg
      601 tatcacatga tattcacaag ctttatgtta ctagtacatc agcaaaagag gaatttgata
      661 caaatgatca aactttttat aagataactg gtaaacattc aaaagtaata agaatacctt
      721 atggaagcaa accatataca ccacaagcct cttatcaggc tttagttgat gctggatata
      781 aaatttggga ctgggactta gatacagaag actggagatc taattcttct caaattgtac
      841 aaaatgtaaa aaatcatatt aagaatagaa aaggtgaaga taaagaccaa cttgtagttt
      901 taatgcatga gaaaaaacag agtgcagaag ctttagattc agttttaaaa ttcttatcag
      961 atgaaggtta tgaatttgcg ccagtagatc aaaaccaaat accaaaaaat tattggttac
     1021 gtaatttaga gtagttaaaa tataagaata ttgattgata tttttagaaa ttgagaataa
     1081 aaatgagtga ggtgtaggta tattgaataa aaaactttat ataggaatgg tcggaatatt
     1141 gtctttgatg atggttggtt gtaataaaag tttagcaaaa gtaaatgatg tggaaatcac
     1201 aaaagaacag tacaaaaaaa caaaagcagt tttatctgct actaataatt atataaatgg
     1261 gcagtcttta gatgaattag aaaaaacttt ggataaaaaa ggtagaaata aattagaaaa
     1321 tgttataata tcgtttatgg tggataatga acttttatat caagaggcta aggataaagg
     1381 actaactcca agtaaaagtg aagtagactc aaaatatcaa gaactagaag ataagatgaa
     1441 tttgaataca agctataagg aaaaaatgga caaagcaggt gttgataaag attatttaaa
     1501 acaagaaata tctagagatt tagcaataga taaaaataaa aaggcctttg aagatagaat
     1561 aaacataagt gataatgata tggaagccta ctatacaagt cataaaaaag attttaatgt
     1621 agaagaagta agtgcttctc agatacttat atcgacttta gataaaaata aaaaagaagt
     1681 aagcaaagat aaaaaagaag ctttgaagaa gaaggcagat aatatattga ctaaaatcaa
     1741 aaatggagaa agttttgaaa gtttggctaa aaaatattcg gatgataaag caactggaaa
     1801 aaatggagga cagctaggat attttacaaa ggatgataaa aatgctgagt ttacaaagga
     1861 agtgttcaaa ttaaagaaaa atgaagtttc aaatgtgttt gaaactagtt atggatatca
     1921 tatagtcaaa gttactgata aaagagaaag acaaaaaagt tttaatgagt gtcagagttt
     1981 aataagagaa tctattttaa atgaaaagta tattgagcat ataaaaaaac tgaatgaaga
     2041 tgctaaaata gacagataga atttctaaca gctcaatcct agcctgtaat cggagggaga
     2101 ttttacagga atgtttaaga gtttaaagag aacgaaagtt gagaaataca ttatcgataa
     2161 taaagactct ttttatagaa tagcatatag ttatacgaag aatgaagaag atgccttaga
     2221 tgtggttcaa gaagccatgt ataaggcact atactcagtg gaaaatatta aagaagtaaa
     2281 ttatataaag acatggtttt ataaaatttt agttagaacg agtatagatt ttattagaaa
     2341 aaaccgtaaa tataataata tgacagatat tgaccttata gatgagactg gagagtatga
     2401 taaatatact gatttggatt taagaagagc tttggaagaa ttacctattg aatacaaatc
     2461 tattataata ttaagatttt ttgaagattt aaagattgaa gaagtagcaa taatattgga
     2521 tgaaaatgtg aatactgtta aaactaggtt atatactgct ttaaaaaaac taaaattaaa
     2581 aattgaagag tagggataaa aagaatgaca agcaaagaaa ggttaaataa gttaaaagat
     2641 gagtatcata aaattccaat acccaagaaa ttagatacta taataaatca tgaaaaaata
     2701 gaaaacatat atcgagagaa aaataaaaaa gtaaatagat taagatttaa agttgcaata
     2761 gcctttgcat gtatatttac agtattagta aatataagtc ctgtatttgc agacaatttt
     2821 tctaagatac ctgtaattgg agcaatagta gaagtaataa ctataaaaaa ttatagttta
     2881 aaaagtgaaa attatgaagc tgagattgat attcctaaaa tacgaggttt aaaagataaa
     2941 aacttagaac aaagattaaa cagtagtttt atggaagatg gaaaaaggct ttatcatcaa
     3001 ttccaagaga gaatggagaa gatacaatca tcaaagaata aaggatataa atctttaagt
     3061 ttgagttata gtgtaaaaaa taattctaaa aaatttctat caatagagat gacaaaaaat
     3121 gagatagagg cttcatcata tgtgagtaag gtacattata ctattgataa aaaaagacaa
     3181 atagttttga ctttgccaat gctttttaaa gatgataagt atataaaagt tataagtgat
     3241 aatataaaag aacaaatgag agaacaaatg aaaaaggata gtacaaaatc ctattttata
     3301 gatcaaaaaa aagacttgcc tgttgaagat tttaaaacaa ttaataagta tcaggatttt
     3361 tattttaata aaaatgaaga ccttgtaatt tgttttgatg aatatgaagt agcacctggt
     3421 tatatgggcg cagttgagtt tgtaatacca tataaagtaa taaaagattt gtaaattgaa
     3481 tagtagttta tatataaatc ttttaaggag gacaatatga ctacatataa aaaacttaaa
     3541 aaatctatat ttaaaattgc tatattttca atatgcttat tagcttcatt agtagataca
     3601 aaccctatat ttgcaggtag ttcctttaat gaaacttcaa atagtgatac gataaaggtg
     3661 ttaaatgcta agagtatcaa ctttaaacaa gacaattatg aagtgaatgt gagtattcct
     3721 aaaataaaag gtttaaaaga taaaacttta gagaataaat taaacaatga gtttttgcaa
     3781 gatgggaaaa aaatttacag tgaatttcaa aataaaatga aaaaggaaga taataagggt
     3841 catagatacc taaatgtaat ccataatgtc aaaaataatt ctaaggactt tttatcaata
     3901 gaaatgacaa aagaagagat agaagcttcc tcatctataa ctaagaagca ttacactgtt
     3961 gataaaaata gaggaatact attaaattta ccaatatttt ttaaagatag tagatatatt
     4021 agagctataa gtgataatat aaaagagcag atgagaaatc aaattaagaa agatgataca
     4081 aaatcttatt ttatagacca agataaagac tcgccagtga aagattttga atctataaat
     4141 aagtatcaag atttttattt taataaaaat ggaaatcttg tgattagttt tgatgaatat
     4201 gaagtagctc ctggttatat gggagcagta gaatttgtga taccaaataa agtaataaaa
     4261 agactaagat agatatatag aagattttta gatagactgg taaggagaat tatatgaata
     4321 tatatgaaca agcaataaga ttaatagaaa ataatgagga tttcgcattt gctacaataa
     4381 cgtctcattc aggttcaaca ccacgtgaaa ctggagcaat gatgattgtg aaaaatgatt
     4441 ctactatatt tggctcagta ggaggaggta gtgtagaagc agaatgtata aaacatgcaa
     4501 ttaatgttat aaaaaacaga gaatcaatgt tgtataaatt tactctaaac aaatctgatg
     4561 ttgcaaaact aggaatgata tgtggaggta ctggagaaat acaaatagat tttatagata
     4621 gtaaattaaa aagtaatata gaaaaattta ataagagact aaaggaaaat acaagtaaag
     4681 catatatatt tggtgcaggt catatttcaa gagatgttgc tgtgatacta tctcttttag
     4741 aatttagaac agtagtaata gatgatagag aagaatttgc taatcatgaa aggtttccag
     4801 aatcagaagt agtagtttta gattcttttg aaaacatacc agattttcca actgacgaaa
     4861 atagttatat aattatttta acaagaggtc atctatatga ttcaagtgct ttagaatggg
     4921 ctcttaaaag agaggctggt tatataggta tgattggaag tagaacaaaa attggattga
     4981 cttacgaaaa actcatgaaa aaaggtttta agaaggaaga attatcaaaa gtacatgcac
     5041 caataggcat aaaattgaat gctcaaacac cagctgaaat agctgtatgt atagctgctg
     5101 aacttataaa ttgtagagct aataaggaaa aataaactaa tgaacacagg gagtaaagat
     5161 attgatactt taaaggttaa ttttagaggg attgaaataa aagcagtttt agtatataaa
     5221 aatagaaaaa atatttctat aaaaatagat ccatttggca aaataactgt aatgagtcct
     5281 cctaaaatat ccaaaaaaat aataaaagat ataattattg aaaaaggaga ctggatactt
     5341 aaaaagtcag aaaaatgtaa aggaagagag gaagtatata aacaaagaat gtttataact
     5401 ggcgaaaaat ttctttattt aggtgaagag taccatttag ttataaaaga actgttgaga
     5461 gattctgtca aaaaaagtaa ctgtagaata actattaacg aatatcaaat agttgttcag
     5521 acaaatgata caagtacaga ttttattaaa aaatctttaa aatcttggta taagatggaa
     5581 agtgaaagaa tagtattaga gagaatagat tttcttaaat taaattgtga aataatgaag
     5641 caattagttc cagcgagtgt taaggtaaaa gaacaaaata agagatgggg aagttgtaca
     5701 gctcaaaaaa atatttatat aaactcaaaa atatctatgg caagattaga tgtaattgat
     5761 tatgtaataa tacatgagtt tagccattta gtacatatga atcactctaa aaaattctat
     5821 gatttagtaa aatccattat gcccgattat aaagataaag aaaattggct aaaaataaat
     5881 ggatataaaa taataatctg aaaacgatta ttccaaaata taggcgtaaa gtataaaaga
     5941 agtattattg tgaaaaaaat aaataatctg aaaattttaa aaaaagtcat tgacattgta
     6001 aatgataggg aatataatct atattaacaa caaaacagaa ttaaaatata aaattaaaaa
     6061 attaaaagca atgacagaga caacaatact aatagtgtta tccacagaga accaatgttg
     6121 ctgagaattg gtgttaacag tagtataatg atcactctgg agcttttgta ctgaatattt
     6181 ttagtaagta caaacgtatg tcagcgttac atgactaagg ttaaggtaat taattacccg
     6241 acctgaaaaa agaggggtag atgtattttt tatatctaca aataaggtgg taccgcggaa
     6301 tataactttc gtccttataa gtaatattta tattgcttat aaagatgaaa gtttttttat
     6361 tttaagcgat ataaataaat ttaatccata taaaattaat aaaaaaataa taaaaataag
     6421 gagggatttt tatggaaaat actgtatatg ctaaattaaa ccaaggaata gattcttttt
     6481 taagagtggc tatgactcta agaagaagag aggttgatat acaatcaata agtatgactg
     6541 tagataatat gaataattca ggtataaaat taatagtaaa tgaagaaaag acatccttag
     6601 attctgtctt aaaccatatg aaaaaactac atgatataag agagataact gttgaaagaa
     6661 tgagacatta attaaatata ttttataaag ctaaagataa atatgcttat tttaaaataa
     6721 aaaactaaag ataaatatta aataagaaaa atcttagatt aaaatataag tgtcattgaa
     6781 tttattagtt tatcacagtt aataaattta ttagtttgtt gtagctaata aaaactgaaa
     6841 attctaaaat tataattgta agattatata aatttagaat ttaaatctgt tgaataatat
     6901 taaaacaatg ataatgagta tgaatttcta ataaaaataa atatcataaa aataagaaaa
     6961 agtattggga ggaaatgaaa atggcaagaa tgtattatga aaaagatgtg gatttagaag
     7021 ttttaaaaaa taaaaaggtt gcagttttag gttatggaag tcaaggacat gcacatgcac
     7081 aaaatcttag agataacggt gtacatgtag tgataggtct ttatgacgga agtaaatcag
     7141 cacaaaaagc aaaagaagat ggatttgaag ttaagagtgt agcagaagct acaaaagaaa
     7201 gtgatttgac aatgatgtta atgcctgatg aaaaacaaaa gaaagtttat gaagagagtg
     7261 taaaggataa cttaaaagaa ggccagacat tagcttttgc tcatggattc aatatacatt
     7321 ataatcaagt acaaccacca gaatttgttg atgttgtaat ggtagctcct aaaggacctg
     7381 gtcacttagt tagaaatgta tttacaaagg gaagtggagt tcctgcacta tttgcagtat
     7441 atcaagacta cactaaaaaa gctacagaga cagttttagc atatgcaaaa gggataggag
     7501 caacaagagc aggtgtttta gaaactactt ttaaagaaga aacagaaaca gatttatttg
     7561 gagaacaatc agtactttgt ggtggaataa gtgaacttat aaaattggga tataaaacgc
     7621 ttgtagatgc aggttatcaa aaagaagtag catattttga atgtttacat gaaatgaaac
     7681 taatagtcga ccttatatat gaaggtggat ttgaaagaat gagatatagt ataagtgaca
     7741 cagcagaata tggagattat gtatctggta aaagggtaat aactgatgtt gcaaaacaag
     7801 gtatgcaaaa tgttttagag gatatacaaa atggaaaatt tgcaaaagcg tggattaaag
     7861 aaaatgaaga aggacgtgaa aacttcctaa aaactagaga agaagaatat aatacagaaa
     7921 ttgccgaagt tggtagaaat ctaagaagta tgatgtcatt cttaaaataa atatatatta
     7981 aatggattag tttgagggaa agagtggtgt atagtagtgc gtatgaatgg agcaaaggta
     8041 attttagaat gtttaaagaa agagggaatt gacacaatat ttggatatcc tggtggagcc
     8101 gtgatacctc tttatgatgc tctatacgat tattcagatg attttaaaca tataaggaca
     8161 tcccatgaac aaggtttagt tcatgctgca gatgggtacg caagaagtac taatacagta
     8221 ggagtttgtt ttacaacatc tggtccagga gcaacaaatg ccataactgg aatagcaaca
     8281 gcttttatgg attcttcacc tatggttgta atttcagggc aggtgccaac tagtttatta
     8341 ggaaaagatt cttttcaaga aattgacata actggagcaa cattatctat gacaaaacat
     8401 aattatttag ttagaaacac taaagaatta gttcctacaa taaaagaagc ttttagagtt
     8461 gcaaattcag gtagaaaagg acctgtactt gtagatgttc caaaagattt atttttagca
     8521 gaaatggatt ttagtggtga ggactatgat ttatgtcaaa tagatgatta tatggattat
     8581 aagagtgatt ttgatctaga tgatgaaact aatattaagc ttttaaatga agcaatagat
     8641 atcataaaag aatctaaaaa gcctataata tatgcaggtg gaggtgttaa atcttcagac
     8701 agtgaggaaa ttcttgaaaa atttgcaact aagatagata cacccgtact aaatacactt
     8761 atggggcttg gaaatataga tagaaagaat gaattatctc ttggaatggt tggaatgcat
     8821 ggaagtagag aaagtaattt agcactttct aattcagatt tagtaatagc gataggtgcg
     8881 agatttagtg atagagtgat aagtaaaagt tctgaatttg ctaaaaatgc aaaaataata
     8941 cacatagaca tagacccatc tgaaataagt aaaaatatag aatctaatgt atctttggtt
     9001 ggagatgtaa agctagtcct tagtttatta attgaaaggg ttgaaagtaa gaataatagc
     9061 aattggaaag aagagataaa gagatttaga aagagtgagg gtgtacaaac atatgaattt
     9121 catccacaaa atatacttaa aaaaataaat gaaaaatatg aaacattaaa gaaacccact
     9181 gtggtagtaa ctgatgtcgg tcaacatcaa atgtgggctg ctaagtactg gaattttaaa
     9241 ggtaataaaa gttttataac atcagcaggg ttaggaacta tgggatttgg tttaggtgca
     9301 gctattggaa ctaaagttgg taatgtagat aaaaatgtgg ttttagtcac tggtgatgga
     9361 agttttagaa tgaactgtaa tgaactagct acagttgcaa attataatgt acctatgctt
     9421 attttactac tcaataatag aacattagga atggtaaggc aatggcaaaa gttattttcc
     9481 aatcaaagat actctcaaac tgacattaat gaaaatgtgg attatgttaa actcgtaaat
     9541 gcatataaca ttgatggata taaggtatcc agtatggaag aattaggaaa ggcgctagat
     9601 atgatagatt ttaataaacc tgtatttttg caatgtgata tagataagga ttatgatgtt
     9661 tatcctattg tagcacctaa tgatgcattg gaaaatttga tatgtaacta aaacttatac
     9721 tgaatattga taaaattttt tattaaattt ttattagtat tcagtttttg ttgtttagag
     9781 atagattggc attttttatt tttgcaaata aatcaatgac tatataatta agaacttatt
     9841 aaattttaat tatacaattt agaatagtat tacaacttaa gtaaataata attttgaata
     9901 ataatttacg gaggtattca tatgtttaaa cacgataaag cattattaaa agaagtaaaa
     9961 gtagaaagac caaatcctca atatgctgtt ttaatgcaag aacagttggg tggtgcaaat
    10021 ggagagttaa aagcagctat gcaatacttg tcccaaagtt ttagaataaa agaccctcaa
    10081 ataaaagatt tatttcttga tatagcagca gaagagctta gccatatgga aatggtagca
    10141 caaactataa atttattaaa tggacatgat gttgattata atgcagttaa tacaggcgaa
    10201 atagaaacac atgtattaac tggtctatca ccagtcttaa taaactcatc aggagctcct
    10261 tggactgcta attacgttac agttacaggg gatttagtag ctgacttact ttcaaatata
    10321 gcttctgaac aaagagctaa ggtagtttat gagtacttgt atagacaaat agatgataaa
    10381 tatgtaaaag aaacaataga tttcttactt aatagagaag aagcacataa tgctctattt
    10441 agagatgctt taaataaagt taaagataca ggttcaaata gagattttgg agttacagaa
    10501 gattctaaat tatatttcga tttgtctact ccagggccaa atcacgatac taagatagat
    10561 attaatcctc cttcttttga aaaaccttta aaaaaataat ttgatatttt ttattttgtt
    10621 ttagtagtta taaaaattat ttactagaag aattaattat atgaaaaatt aaatatgtat
    10681 agttaaaaat gggtatacac ttattataag tgtatatcta tttgtttatt gcaaaacata
    10741 taataaaata acaaatgagg tggtattttg gacagtaata gaaaacctaa taatctaata
    10801 aatgaaaaat ctccatatct tttacagcat gcttataatc ctataaactg gtatagttgg
    10861 aatgatgagg cttttaaaaa agcaaaagaa gaggataaac caatattttt aagtgtagga
    10921 tattcaacat gtcattggtg ccatgttatg gaaaaagaat cttttgaaga tgaagaagtt
    10981 gctgaaataa tgaatagaaa ctttgtggca ataaaagtag ataaagagga aagacctgat
    11041 gtagatagtg tgtatatgac agtttgccaa gctatgacag gtagtggtgg atggccaatg
    11101 accataataa tgacaccaga taaaaaacct ttttttgcgg gaacatattt cccaaaatat
    11161 tcaaggtata atagacctgg agttatagat ttattagaga atgtatctga aaagtggaat
    11221 acaagcagag atatattaat aaaatctgga gatgaaataa tagaagctct taaggatgat
    11281 tttggtgtaa aaaatactga gggtgattta tcaaaagaga tgttgagttc atcagttaga
    11341 gtatttaagg ctatttatga tgaaaagtat ggtggttttg gtaatgctcc taaatttcca
    11401 agtcctcaga atcttatgtt tttaatgaaa tattatagta tagaaaaaga caaagatgtg
    11461 ttaaaaatgg tggaaaaaac tttagatgga atgtatagag gaggcttgtt tgatcatatt
    11521 ggatttgggt tttcaagata ctctactgat aaaaagtggt tggctcctca ttttgaaaaa
    11581 atgctatatg ataatgctat gcttactata gcttttttag atgcgtataa aataacaaag
    11641 aaagagttgt ataaagaaat tgctataaaa actatagatt atgtagttag agagatgaag
    11701 gataaagaag gtgggtttta ttcagcacag gatgctgata gtgaaggtga agaaggtaaa
    11761 ttttatacat ttaatcctct tgagataatt gaagttttgg gtgaagaaga tggaattttc
    11821 tttaataatt attttgatat aactagtagt ggaaattttg aaggcaaaag tattcctaat
    11881 cttataaaaa ataaagaata tgagagacat aatgaaaaga tagctgacct tagtaaaaag
    11941 gtatttgaat atagaaaaga aagaacatct ttgcataaag atgacaagat acttacatca
    12001 tggaatgcat tgatgatagt tgctttaaca aaggcatata gtactttaaa aaatgacata
    12061 tatttagagt attcaaataa gtgtcttaac tttataaata ataatcttgt aaatgagtca
    12121 ggtagattat tagctagata tagagatgga agttccgact atttagcata tttagatgat
    12181 tatgcttttt taatctgggc gtatatagag ctttatgagt caacatttaa tatgaaatat
    12241 ttagaaaagg ctttaaattt gaatgaaagc tgtatcaacc ttttttggga ttatgaaaaa
    12301 agtggatttt atatatatgg taaagatagt gaaaatttaa tagcaaggcc aaaagatttg
    12361 tatgatggag ctattccatc aggaaattct gttcaattat acaatcttat aagacttgcg
    12421 aaaattactg gagataatag acttgaagaa atgtcatata aacaattaaa actgtatgtg
    12481 gataatgtaa agagttctcc agctggctac agcttttata tgctttcatt gatgtttgaa
    12541 ctttattcta ctaaggaaat tatatgtatt tttaaggaag attctgattt aattgccttt
    12601 aaagaattga taagtgaaaa ttttatacca aatgcaactt ttttagcaaa gaaatacaat
    12661 gaagaaaaca ctattatagg ttttttaaat aactatagat tgaaggatga taagattagt
    12721 tattacgtgt gtcaaagtaa tagttgtagt caaccaatta atgatttaca aaaattaaaa
    12781 gatatgattt taggagtaga ataatcataa tagtgaaaaa ggagatgctt taaaataaaa
    12841 gtctaatcat ctcctttttt tgtacaaaaa acagaaaaag agttcctgag aacacctaat
    12901 ctatgtaatg attgttttgt taaaaaataa aaatgacatt atagttatat aaaatattgt
    12961 actataaaaa agtagtatta tagatgcgat agtattaatc gtttttattt actctctttc
    13021 ttctttttgc aaattcttca agtgcttcag atttaatttt atctatttct tcatattcca
    13081 taaaattagc atcttcttca aagccatctt gttcttcacc attaaaacga tacatttttt
    13141 ttagtaaact ccataaggtc tctaattcat ctttggtaaa ttcgtgaaat acatctgcca
    13201 taaagtttat tccagtccta ctacatgtta ccataacctt ttttcctaag tctgtaactt
    13261 taacattaat tgctctctta tcatgaggac ttggaattac atcaacatat ccattttttt
    13321 caagattagc aactagtcta tttatatttt gtttacttgt acccattttt cttgcaatat
    13381 tatttaatgt tgtttcttcc tctggtaaat gaagaattga taaaatagtc atatactgtc
    13441 ttgaagtaag tataccaaaa tatttatctc cttctatttg tattttgttt gtaagggtaa
    13501 aaagagttgc atatgtttga ttcattaaaa agagttcttt tagttcattt gaataatcca
    13561 tgaaatatat ctcctttatt tttagatgtt gttttgtcga aaacagaata tcatgaatta
    13621 attctaatgt caatatggtg tcaaaatata ttaaagtatt acttttttat gtaactatta
    13681 attaattaaa ttattgtata attttatgta aaaacttcta tgtaccaatg ataacttcta
    13741 tgaggaatta ataattctta aattttttag aaagattttg acttgaaaat acttgtaata
    13801 taatatataa attaatatac tgaataaaat caatctggag ggataattat gaagtgtaca
    13861 aactttactt tcaaaggaga ggaaggttta gatatatata catataaatg ggaagatgaa
    13921 aatataaaaa aaccaaaggc agttatccaa attgcacatg gaatggctga aactgcacaa
    13981 aggtatgaga cttttgccaa ggtacttact aaaaatggtt atatagtata tatcaatgac
    14041 catagaggtc atgggaaaac tgcaaagata atagaaaatg ttggacattt agctgaaaaa
    14101 gaaggattta gatgtcttgt agaggacatg tatactttaa caaatattat aaagaaagaa
    14161 aatgaagatc tgccaattta tctatttgga cacagcatgg gttcatttgc aagtcaaaga
    14221 tatataatgg attacagtaa taatttatct ggtcttatac tatgtggttc aaatggaaag
    14281 caaggaatca ttctaaatct tgctcatctg ataatcaatc gtgagattaa aaagtatgga
    14341 agacgttcta aaagtaataa aataaataat ttgatatttg gcggcgaaat aataagaaga
    14401 aatgaaaaga ctaagtttga ctggttaagt agagataaag aacaagttga aaaatatata
    14461 aatgacccat tttgtggagt agtgtgtagt tgtggctttt tttatgattt agttcaaggg
    14521 ttaaaagaaa ttgaggataa agaaaactta aaaaaagtgc cacttgatat acctatttac
    14581 ataatatctg gagataaaga ccccatagga aaaaatggta aaggagtctt aagactaagg
    14641 gatagatata taaaattagg agtaaaagat gtaacttgca aactttacaa agatggaagg
    14701 catgaattgt tgaatgaaat aaatagagaa gaagtttttg aggatataat ttgttggtta
    14761 aataataaaa tagaaatatt gtaataaaaa aatataatga tatatttact ttaaatttat
    14821 ttatatataa tatttgaaaa gtggattatg tatatgcaaa gtagaaaaga gagagcccag
    14881 aagtgatttt ttgctataat gccatttatt gaataggtgg gtgttattat aaataaatta
    14941 ttaacactca gtattattat cattgtatat ggaatttatt ttatttaaaa ttccttgaaa
    15001 gtttaaatat agtaagttgt agtaaagaga aaatataaat atttaaatgc tatttaatgt
    15061 gaatctatta attaggttat acttaaactt aattaataga ttttatattt caagacaatg
    15121 agatagggtt ttagattaaa atataaccaa tggttaaatt acagacattt tgttaacttt
    15181 ccctcgattt actagtaaat atttttttta tatacttaaa ttacaacaga tgtactaggg
    15241 aggtatatat gcaaattaaa ataggtaaag tcatacagtg tcttagaaaa gaacgaaatt
    15301 taactcaaga acaattagca aagtttatag gtgtgtcaac accagcagta tctaagtggg
    15361 agagtggaaa ttcttaccca gatatagaat tattgccttt attagcagac ttttttaatg
    15421 tgtctattga taagctttta aattacaaga ttgatttaag tgaagaagaa gtaatgaaaa
    15481 tttataaaga attagaatca ggctttgcta gaattgaaat tgatttatca acagaagaac
    15541 caaaagaaga atttagacaa gatttagaat ctgtaaaaaa gctttccaat atgtatatag
    15601 agaagtatcc taaaagctat ttattgaagc taaggatatg ttcgctgtat cagatgtatt
    15661 catataaatt tggtaagagt gaacttaatg acagagtaaa agaaacaacg aatattcttg
    15721 aagatatagt gagaaataca gatgatatcc aaataaaaga aacagctctt attatattat
    15781 caaacgcata ttgtatgttg gaggattatg aaaaggcaga gttgtattta aatatgatac
    15841 ataagtcaat aggggataca agtgttaatt tagctatgat atatttaaag caaaatagac
    15901 ttgaagaagc agaaatattg cttcaaaata aattgtttag taatgtattt aatataagca
    15961 tggattgtaa gggaataatt aatgtataca aaaatcaata caaggaatta aagaaaaaat
    16021 tggagaatag aaattttaat aaaaatgcaa ttgagagaga aatggagtat ataaaaaata
    16081 agttgctggg gtatgcaaat ctttctttag aaataaaaaa aatgctcagt gaagataaag
    16141 gtgcattttt tagtatgtat atggattata tggaattatc tctaattttt ttatttttta
    16201 acatgaaaga agaagcaaaa aaagctttat atagtctaaa agaaatatta gaaaaatatc
    16261 ctatacatga aagtttggat gtaagtcaaa tgagattttt tgataaggta gagtctaaaa
    16321 atttatatac ttttaatata tatacaaact tgttaattgt acttaatgat gacagttata
    16381 atgagcttag agaagaacca atttttaagg atgtaattga aaagatttta gatatggaaa
    16441 aaatgttgaa aaataaagaa taagtaaaaa tttaatatag gacttaaaga ttaatcaaat
    16501 taaaagaaag tgtttcaaaa taaaaatttg aatcaccttt tttgtcggtc ttaaacagtt
    16561 atttagaaaa atagaggttt caaaatagaa atctgaatta tctgtattat tacacaaaaa
    16621 tagaaaccta aatttataaa aatagaataa aatttgttat ataaaatgta attttgtcta
    16681 taaaatctca cctgttttct taactttaag cgtgaaaaag aggatgtatc atgaattttt
    16741 tagttcatga tgcatcctgc tttgtatgaa atccaatata tctttattat ttaaacaatg
    16801 gaaaagagtc ttatttatat tttgagacat ctcttttatt taattatgaa gttttataca
    16861 gttcaataaa taggaattat atttagaaac tcatgacatt atttcttaga tagtgattaa
    16921 atttatcata ttccatttta gataactttt ctgggtaaga cattacaatc aggttatgcc
    16981 cattaaattt tttatggtat ggtggatgaa tgtgttcata attatttgtc ttatatttta
    17041 ctctttcata gaacgccttg cgataaattg aaatctcatc aattggaagg tcaatttcta
    17101 aaataatagt ttttcctttc ttatttaaaa gctctaacgc tctttgccca tatctattat
    17161 tgcgaatttc tggaaaaatg cagaaatgtt ctacatagat aaagttatct gtttcccaac
    17221 ataaaattat gccgacaaac tggtttttat cataaattaa attgaattga tattctttat
    17281 ttttcaaaat ttctgcttgc aaagaagatt ttctttgttt atgaaatggg aaactaattt
    17341 tatataattc catagccttg taatataatt tattttcact ggttcttaac cgttcaaatt
    17401 tcactttact cctcctaatc acgaaaattc caatttattt atgagttcat tttaccatag
    17461 tcagtatttg catacaatgt gttttaagtt gagtgtcatt tctttctttt atttatgctt
    17521 atatctcata gtctaagaga taattgctta agacataact aaaaactatt atattaagct
    17581 ttgataatct aaaaacagta tatttatgga cttttctagg tctaaaagta atcttaataa
    17641 ttttagaagt tacaaaaaag ttacataaat ttggaaagct ataatataca tatataatat
    17701 aaggttaaaa ctgtaatgaa taatattaat ttaaaatttg aacatggaag gagattatta
    17761 tggagaaaag agaaaagagg ataataatat catctttatt aagtgtatca attttaatgg
    17821 gtttggcaag tatatattct atattaaata aagaagacat aagtttaacc gttaaaggtc
    17881 aagagcaaaa agtatcttcc tttaagaaaa cagttgagga acttttggat gaacagggtg
    17941 taaagtataa ttctgaagat aagattaatc caagtttaga tacagagtta aaagatgata
    18001 tgaaaataaa agttgttaag gtaactaaaa gtaaaaaaga agagattgaa aaaattccat
    18061 ttgatacaaa gcatgtaaat gatagtaatt tgttaaaagg aaaatctaaa gtttatcaag
    18121 aaggtcaaga aggagaaaaa aaactagtct ataatttaac ttaccatgat ggcaagttag
    18181 tcaaaaaagt cttatcaaaa gaagtaatat ctaaggagcc aactacaaaa attataaaat
    18241 atggaactaa agaaaaagta ctaatagcat caagaggagc aaatataaga ggaggcaaac
    18301 atatgaaagt ggttgccact gcatatgcag gagatacaat aacatctact ggtacaactc
    18361 caagatgggg tgttattgca gttgacccac gtgtaatacc atatggaaca aaagtatata
    18421 tacctaaact gggtatgact tttgtagcag aagattgtgg aggtgcaatt aaaggtaata
    18481 gaatagacat ttttatgaac agtgaaggaa aagcttcaaa ttggggaagg aaaagtatag
    18541 atatatatct acattaaatt agtgaataga ataactttac ttgaaggaca aaaaagtata
    18601 aaatatgaat taactaaact gttagattat tcttaaaaca taaaaatata tctaaggggg
    18661 gataagatga aaatagtctg cttaggagac agtttaacgt atggatttgg agtatcacga
    18721 agtaattcat ggacaaacat tgtaaataaa gagactcgat tagaaatagt aaataagggt
    18781 ataaatggtg atacaacaag tggtatgtta gctaggttta atgaagatgt tgtaaaaaac
    18841 tctcctgata tagtttttat aatgggtggc accaatgatt ttattgctgg tgcgggaaat
    18901 gaaattatta attctaatat aatggctatg gtgcatcagt catattcaaa aaatattata
    18961 cctataatag gtataccatt aaaaccagat attccaaatg ttagggaaga ttggagttgt
    19021 tttactgatt ttaatatagt atctcaaaac ttagaatcat atagttattg gataaaaaaa
    19081 ttttgtatga catttaatac taatttcgta gatttttatt cagaatataa taaaaatatg
    19141 gaactagaag gatataaaaa attatatttt gatgggttac atccaaccaa agagggtcat
    19201 agaataatgg cagacatttt tattaattca attaataagt atatagaaga gttatagatt
    19261 acattaaaat gcttgtttat gcgtcagttg caataaatac atacagtaga gaggggttta
    19321 ttatggatta tactaaaact tatgaagagt ggataaaggg ttcatatttt gatgaagata
    19381 caaagctaga gttggagaat ataaaaaata atgaaaaaga aatagaggat agattctata
    19441 aggatttaga atttgggact gcgggtctta gaggaataat tgaagcagga acgaatagaa
    19501 taaataagta cacagttaga agagctactt ttggattggc aaactatata ttagaaaaca
    19561 caacaaaaga agaaacaagt agaggagtag taatagctca tgataataga cacaagtcta
    19621 gacaattctg tatagaatct gcaaatactt tggcagcatg tggtataaaa gcatatatat
    19681 ttgatagttt gagaactact ccagaattat cttttgctgt tagaagctta aatgcaatcg
    19741 ctggtatagt cataacagca agccataatc ctccagaata taatggatat aaggtatact
    19801 gggaagatgg agcacaagtt atgccagaga ttgcaaatgc aataacagag aaagtaaata
    19861 gtatacatga ttatagtaca ataccaacct taacggaagc taataaaaat ttggtagttt
    19921 tgttggacga atcacaagat actaaattta tagaagcagt aaaaagccaa attataagaa
    19981 aagatttagt taaaaatgta ggtaaaagct ttaagatagt ttatacacct ctttgtggaa
    20041 cagggaatgt tccaataaga agagcattga aagaagttgg ttttgaaaat ataattgtag
    20101 ttccagaaga agaaaatcca gacccaaatt ttgcagggct tgattatcca aatccagaag
    20161 agaaaaaagc tttaaataga ggtatattac tcgcaaaaga aaaaggagca gaccttgtaa
    20221 tagcaactga cccagattgt gatagagtag gtgtagcagt aaaaactact acaggtgagt
    20281 atgctttact tacaggaaat caaattggtg gtatgctgac acattatata atagaaggat
    20341 taaaagaaaa taataaatta aaagaaaatc ctacaataat aaaaaccata gttacatcag
    20401 aatttggagc agatatagct aaagcaaata atgttgatgt attaaatgtt cttacaggtt
    20461 ttaaatttat tggtgaaaaa ataaaattat ttgaacaaaa taaaaataga agttatgtat
    20521 ttggatatga agaaagttat ggttacttag ttgggactca tgctagagat aaagatggtg
    20581 tagtttcttc attgttaata tctgaaatgg cagcatttta ctattcaaaa ggtatgagtt
    20641 tatatgatgg cttaatagaa ctttataaaa agtatggatt tttcaaagaa cagactatat
    20701 ctttgacttt aaaaggtata gaaggtgtag aaaagataaa agaaataata tcttatttta
    20761 gagaaaatca aattgattat ataaatagta ttaaagtagt tgataaaaaa gattataaaa
    20821 atggtataga taatcttcct aaatctgatg ttttaaaata ctttttggaa gatgaatctt
    20881 gggtagctat aagaccatct ggaacagaac caaaacttaa attttacata gctgtaaaag
    20941 gagctagtga tattgaagca gataaaaaga ttcaaggttt gaaaaagtat atagatgaca
    21001 tggtcgaaaa attaaaataa aatattatta ttttataaga taattttgta ataatattta
    21061 tttagctgaa aattataatt tactttcatt gagtagaata tggaaatgtt agtagaattg
    21121 ttattttcag cttttatttg tgttattttt acttaaataa ttatattata gaatgaatct
    21181 gagtataatt aatattgtat aatgaaatta atttgataga aaaggggata tttatgtttg
    21241 gtatagataa gaaattgatt gaatatataa ttgcaaaatc caattacagt gaattaagtt
    21301 caaagtcagc agaagtatct ttcttcttaa tgttgtccat atttcctttt ttaatattta
    21361 caataagttc tatagcttat ataccgattc ttcatttaaa taagtatata gctttgttta
    21421 gaaatatgat gccagaaggt gcttttgctg tgttatcttc aatcatagtt tctgcaatag
    21481 acaatagaaa cctgaaattt ttagcagtca gttttgtact tactatgtgg acattttcaa
    21541 gagcagtaaa agctcttatt aaaggaatga atagagccta taaagttaaa gaaactaggt
    21601 ctttttttaa aattttatct atatcatttt tatttacaat aatgcttttg gtccttattt
    21661 ttttatctat gatattttta gtttatgggg aaaaaatagg ttattttatt tttaatcttg
    21721 taggattgga tgagatattt ataaaaatat gggatattct aagatacact gtaggtataa
    21781 taacaataat agtgatattt acacttttat ataaatacac tccaaataaa aagttaacta
    21841 ttaaagaatc tgcgccaggt gctatttttg ctacttttgc ttggttttta gtatcatttt
    21901 tatattctta ttatacaaat tactatgcta actatgaagt aatctatgga agtatagctg
    21961 aaataattgt acttatgacc tggatgtatt ttagtagttg gtctatcgtt attggctatg
    22021 aggtaaattc aagattgtat tttagaaaga taagacatga aatgttaaag taaagtattt
    22081 aaaataaaag agagtattta gtattataaa tgtaattgaa ttaataaaga aatattgaca
    22141 tttaagttaa aaagtctaga atttttctgg gcttttttta tttttagtat aacttgacaa
    22201 attttattta atgggatagt tttaatttat aatcatcttt aaaaagatga ctattgataa
    22261 gtaagagata aaattggtaa gtatcaaaaa atatactaaa taaaaattat agtcctaatg
    22321 agttaatttc tgtattttca aataatgcag tatttagtca tgtgttttta tatgcgcctg
    22381 tatttctaat taccaatata ttagaataag ctactgtaat tagatttaga aaaaagcgac
    22441 ataaataatt ataaacaaat aataaattac ataaaattaa atttattcac aagtatatct
    22501 tataaaatat gttataatta atagaaaatt gtcatataat taattaagta gtattattat
    22561 aaataacatt gaaaggtagt ggttagaata tggataccca taataaatat gtaaatttta
    22621 taaaaaatat acctgtacct tttttatatt gtagaattgt aaagagacaa gaagatatag
    22681 aatatcgagt agaatatata agtaagggta tgggcaaagt attacaatta gaagaaggta
    22741 tttgtgataa aaatatacta gatgtattgc ctgtatttaa gtctaaaaaa tatttcaaag
    22801 agttgttctc aaatgaagtg gattgtataa aaaggtacat tccgacactt aaaaattgga
    22861 taaatataaa aaaacagata ataggggatt catacataat tttgtatttt ggtaaaattg
    22921 tatttgatta tagacagata atcgattcct ttgataaaaa ggaaaaagta gcttatatta
    22981 aagacgaaga aggcacatat atagattgca gtgaaaattt aataccaata ttaaataaca
    23041 acattaaaac aacgaaagat atctttggta aaaatgatat agaagtatgg ggagagaata
    23101 caggaaaatt atttagagat gattatagag aaggagtatc tagtaaaaaa agatttttac
    23161 aaaatctttt tgaatatgaa gaaacatttt ttatggttga aaaatatttt ttgtatgatg
    23221 aagacgaact tttaggtact atagggatag tagacaatat aatatatagt ggttacagta
    23281 atagaaatta taactcaaaa gatttaatga agatgataga acactcaatt ccagagaata
    23341 tgttttataa agatgtgtat ggaaattata ttggatttaa ttcaggtttt ttgaatttag
    23401 cttgtatgaa taaagaagaa ttattaggga agaatagtta taaaatatca gaagaagaag
    23461 ctttaataga taagatattt gaaagtgata agggtgtagt tgaaaataaa aaagtagtta
    23521 catttgagtt aaatataagt atgaatgatg aaaacaagtg tattgagatt acaaaaaggc
    23581 cgttttttga tagctatgga agtgttattg gaataattgg aacagcaagg gatataagta
    23641 gaagaaaaag attagaagag gaaatggata aaactaggat ggaatttttt gcaaatttat
    23701 cccatgaact tagaacgcct ataaacttaa tatcatcttc attacaagtg atagaaaaaa
    23761 aagaagcaga tttaatagaa tcaaatgata ctttaaaaag aaatttaggt ataataaaac
    23821 aaaatggaaa tagaatatta agattagtaa ataatgttat agattttaca aaaatgcaat
    23881 caggatattt agattttaaa ccagaagaat ctgatataat tgcatttata gaggagatat
    23941 gtatgtctgt agctgatttt gcaagccaaa ataatataca acttacattt gatacagaaa
    24001 tagaagaatt ttcaatgtta tttgattcag aaaaattgga aagaataata ttaaatttac
    24061 tttctaatgg aattaagtac aataagaaag atggaaaaat aaatatattt ttatatgtga
    24121 aagataatgt atttaatatg aaaatatctg atagtggaat aggaatacca aaagaaaaaa
    24181 tagataaaat atttaatagg tttgaacaga ttgacaatga actttcttac agagttaaag
    24241 gaagtggtat tggactttca ttagtgaaat ctttggttga gttgcatgag ggaagtatat
    24301 ctttaaaaag tcaacttggt attggaagtg aatttatagt ttcattgcct gttagaagta
    24361 aaaataatat tgaaaaatat aatcataaaa gagaaatcag caatgaatta agtaaaaaat
    24421 tagaaataga gttctcggat ttataagttt aaatataaaa aataaaattt taaaaaagta
    24481 ttgacaattg atttagaagg gtttaaaata aattacaaca taataaataa aaaggcgatg
    24541 aataggaaga gtaaatctaa ttcgattacc agagagaaga gtccgttggc tggaagactt
    24601 tttaaattag agggattgaa aactacctag gagctacttt ctgaagtcat ctgcattagt
    24661 agattttagt aagaatagtc gttagctgcg ttaaagtgta aagtgagttt tcatgacata
    24721 tgaaaactaa attaggtgga accgcgggaa taatactctc gtccttatgt tataaggatg
    24781 agagtttttt gttatttaaa aatataaaca gtaatcataa taattataga aaatataaaa
    24841 gcaatgaata ggaagagtaa atttaattcg actatcagag aggaaagttc acaggctgaa
    24901 agactttcta agtttgagaa attgaaaact acctaggagc tgtggtctga agttacttta
    24961 aaaagtttta gtaagactaa ccgttaactg cgttaaagtg taaagtggat tttatcaata
    25021 aatgataaaa tcaaattggg tggaaccgcg agtatagact cgtcccttta tatagggatg
    25081 agtctatttt tattgcaata aaagattgaa aataaaaaaa aatcggaaaa ttataaattt
    25141 aggaggaaaa taaaatggag aataaagtga aaattggagt actaggatat ggagttgtag
    25201 gaagtggact tatagatata atagataata ataaagaaaa aagaaatata gaaatagttg
    25261 gaattttagt taataatttg gaaaaacata aggataaaaa atattctaat ataataacta
    25321 ataatataga tgatatattt aataaagata tagatatact tgttgaagtc atgggtggtt
    25381 tagaaccatc attatcttat ataaaaaaag cattaaacaa taaaattcat gttgttacag
    25441 caaataaaga tttgttagct gaatgtggag atgagcttgc aaaattagca agtgaaaata
    25501 aagtaagtat taaatttgaa gcttcagttg caggaggtat accagtatta aaaccaataa
    25561 tagaatcact agaaggaaat aatatagata gtataaatgc aattttaaat ggaactacta
    25621 attttatatt atctaaaatg tatgatgaga atctatctta tgatatggca ttaagacaag
    25681 cacaagaatt aggttttgct gaagcaaatc cagagtcaga tgtattaggg tatgatgctg
    25741 ctagaaaact atcaatatta tcaactctag cttatgacaa tagagtatat tggaaagatg
    25801 tgtatttaga aggaattaca gatatagatg aaaaagatat tgaatatgca aaaaaattaa
    25861 attgtaaaat aaaattgatt ggtcaaagta agtatgaaaa tgataaagta agtgcttttg
    25921 ttagacctgt tttagtagaa aaagataata tccttgctag aatagacaat gagttcaatg
    25981 ctgtcatagt gaatggagat tcagtaggag aagtttcttt tgtaggtaaa ggagctggaa
    26041 gtttagcaac aggaagtgcg gtttattcag atgttataga tataatagat aatagagttt
    26101 caagtataga ttcttttact aaagataaaa tacaagttaa taaaatagtt agagaaaagt
    26161 gtggagcact tcttagattt aaaaaatgta ataaagatga aattctaaat atagttgaga
    26221 attgtttagt aaaattcgat attttaaatg atgatgatga acttgctatt atggtttatg
    26281 ctgattctga atatgaaata aacaacagtt tatgtctaat aaaagataaa ggatactgcg
    26341 aaaaaatgaa taaaatgttg aaaataagtt aaaaaagaaa aaatataagt ttatatgtaa
    26401 catattttga cgattttaat actatatagt atgagatgaa acaaaacatc tcataaaaaa
    26461 attaaaatta aagggagaga tatgttatat ggaaaataaa aaatgttatt cagaagattg
    26521 gtatgaaaga ggagaatcta cagctaaatg gttccaaaat gatagagaag aatatgaaag
    26581 agaagcatat gatgaagata gagaaagaag aggttcaaac tgtggatgtt cagattcagg
    26641 agaaaataga cctagaaact gtgaaagatt tagaagagaa gctgagataa gagaaagaga
    26701 agcaagagaa gcattctgtg aatcttcaga gaaaaagaaa gaggcattag catatgaatg
    26761 tgaagctaga aaattatggg aagaagcaga aaaatactgg gatgaatatt caaaatacaa
    26821 ctataaagga atcgaatatt tagcagaagc tgctagatta tttgatgaag gtatggaatg
    26881 tgaagctaga agaaatggaa ataatggagg aaacaataat aattgttgcc ataaatgcca
    26941 taaatgtaat tgtaactgct gtagaaaata aaaaaattaa ttgacaaatc aatagttaaa
    27001 atatatcata ttatgtataa taaagagaaa attcagaaaa aaataatgat tagaagaaat
    27061 ataagtatat ttattagaca aatagtgctc agttagtgag gtgagtacaa tatgattaaa
    27121 ctcccatcct ctagcttttg atagagggtg ggagttttta tttatttata tatcttaaat
    27181 gaggataata ttgtaaaata aatgagtatg agattgtaaa attaggaggt aaaaatgaaa
    27241 agtattaaaa catcaagtat gaaagaaaag gtagatgaaa agatattaag agagcaagta
    27301 agttctataa tagaagatat aagaaataat aaggatgttg cacttaaaaa atataatgag
    27361 aagtttgata gaaatacaag agatgaattt agaattacta aggaagaaat aaaggaagct
    27421 tataagcatg tagatgacga atttataaat aacttaaaga ttgctgccaa aaatataaga
    27481 gaatttgcta aagctcaaaa actaagtttt gaaaaccctt ttgaaaaaga aatttatcca
    27541 ggtgtaatct taggtcaaac taatattcca atagagtcat gtcttgcata tgttccagga
    27601 ggtcagtatc cattgttttc aacagctctt atgcttataa tacctgcaaa agtagcagga
    27661 gtaaaaagaa tagtggcatg ttcaccaact atgaaaaata ctgaaaagat aaatcctaag
    27721 acattggtag ctatggatat agcaggtgct gatgaaatat atgcaactgg aggagttcaa
    27781 gctatagcag catttacata tggaacagaa aagattaatc cagttgacat aattgtagga
    27841 ccaggaaata agtttgtaac tgaagctaaa agacaatgtt atggacaagt agggattgat
    27901 tttgtcgctg ggccaagtga agttcttata atagcggatg aaacttcaaa tccagtttat
    27961 attgcagctg atttattagc acaatgtgag catgatttaa atgcaagagg aatattattg
    28021 acaaatagct tggaagtagc acaaaaagtt gaaaaaaata tagaaagtat gctaaaagat
    28081 ttgccaacaa aggacatagc atatagttca tgggaaaata atggagagat aatattggtt
    28141 gatgatatgg aagaagccat taaaataagt aatttttatg caccagaaca tttagaagtt
    28201 gcagtaaatg agtataatga tatttgtgat agattaacta actatggttc tttatttatt
    28261 ggaaatcttt ctgctgaggt gtttggtgac tatgtttcag gtaccaatca tacattacca
    28321 acacttaaag catcaagata tactggtggt gtttgggttg gaacttttat caaaacttgt
    28381 acaaagcaga tatttaatga aaaagctata caatcattag cacctgtagc tgaaaagctg
    28441 gcaaaagaag agggattata tgcacatgca aaggctgctg aggttaggtt taaaaagtag
    28501 taaaaaattt tattttggca gtcgagactt agtattagac tgagataagg aaaacgctga
    28561 gaacctgaat aaatactata ttttgtgggt ctcggcgctt cttttatatc tgtatataat
    28621 agaataatac tatctgtgtg tttaaatact aacttaatat ttcttttcta aataatgttt
    28681 atgaaatagt taatattttt agttaatact tgaaaaaaac ttttgctaac tgtgcaaatt
    28741 catctgcatt tgtcaatata gcagggtgtc ctccctgact aaaaatgtaa atacttgctt
    28801 ttgaaataaa agttgccatt tgcttgtact cttcttcaag attatgacga cacatttcgt
    28861 cttccttact tcctgtaaat aaaattggca tttctaattt acacaattct ttatgaaaca
    28921 aagggcgttt ctcgtttgca cattgcaata gagcttttgt atcaagatta acaacattct
    28981 cccaatcttt tccatgacac cattcataga attttcgtgc ttgaatatct tgctttgatt
    29041 tttcccttcc agtgattaga ttatctatga agttatcatt aagagtacgt ccatcaaagc
    29101 tatctgctat aattgcttca actaaatcgg gacgttctaa tccagtatta acagctgccc
    29161 atgctcctcc acttgttcca attaagccaa ctttaccaca attcaaatgt tcaatcagtg
    29221 ctattgtctg taaggcttca tcatgccata tgtcaggaga aaattgagaa actctatccg
    29281 actgtccatt acctaaaaaa tcaattaaga tacatctaaa attttctgca tataatggca
    29341 caagtgcttt aaacatatta gaagaaccag tattaccatg caggaaaatt atcggtttac
    29401 cttgtccata ttctttataa aaacaagaat taccttgata ctgaaaatat gacatatatt
    29461 ttcctccttt ttatttgtta agttaattat aacaatatag gtatgaatga aaaacctcat
    29521 gttctttctt agaataaaaa tgtgccacgc ataaaacgtg acacatttct aattataaat
    29581 tttgctgttt aagtattctt tgtatgcttt tctctgtcaa aaagtatttc tctgataact
    29641 gcttgatact cattccattt tggaaattta tacagatttg agaattacga gaagctaaga
    29701 cttgtttggt gtttgtgttt tcaccccaat gctttttgtg ctcttgcttt ttgggaatat
    29761 aaatcacttt accatctata tattcttgca cttgctcaat cagttcttta ggcagtaagt
    29821 caatcgaatt ttgatagctc attttgcacc tcctaaataa ttattcttta ggattagcaa
    29881 aggctactgc cactcacaat taaaaataaa attttttatt atgtacaata gcctttgcta
    29941 tgcacaagtt ccaattccca taaactaaat actcctttct ggtttgaaaa attcatctga
    30001 acattgattt cgcttttaag atagagttca atgacaaatt tacattttta ttatatatat
    30061 tcagatagat aattgcaact gtctttgtat gaggatattt tattatagtc aatactataa
    30121 tatgtattta tatcatcaaa ctaatatgaa cagttgaaag gaatatgaac aataaaactt
    30181 agtcaggaag aataattatg aagcagacaa tagagaaatt tgattttaaa gttttattga
    30241 aaatttattg acaaatttaa cttgtaaata ttatactaaa cttaataaat aaagtttata
    30301 aagtaaggtg ttgtctatga aaaatatagt tggaaaaccg caaacgataa aaaaagtaaa
    30361 tgaagattta ataaaaaaca ttataaaaaa tgaaggtcct ataacaaaac cagaaatttc
    30421 aagcattact gaattgagtt tagctacagt taataagatt gtagagcaat tagctttgaa
    30481 aaatgaagtt aaagtaagtg gactaagtga atctactgga ggaagaagag ctcaattatt
    30541 tgaaataaat gcaaacttag aacacataat agctttatat tattatagaa attgttgtat
    30601 aggggtagtt gccaatttat taggtgaaat aatttatcaa gaagaattta atataagaat
    30661 ggatacgtat gaaaacgtta accaagatac tttttttgtt atagatactc taataaaaaa
    30721 ggaaaaagga aacaatataa gggctattgg aattggtgtc ccaggagttg taaaaaaagg
    30781 tgtagttaaa aatataccaa ctataaaaag ttgggaaggt ataaatttag aaaatttaat
    30841 aaagagtaaa tacaatacta aagtgtttat agaaaatgat acaaatatta ctactattgg
    30901 gatttatcaa aaacagtata aagacaaata taaaaatatg gctcttatgt atttagaaga
    30961 tggtattggt tcaggtatag ttataaatgg tgaattgtat ttaggttcta caaattttgc
    31021 tggagaatta agttatctaa atatagaatt tggtaagtat aactctaaca acttaaatct
    31081 tgaagattat attatgagtt taagaaaaag atatttagaa agtaaagata ataaatttaa
    31141 gaaagaaatt ttatctataa tatcaaactt tataaggact cttgtatgtg tcctaaatcc
    31201 acaagctatg attatacagt gtagtatatt aaccaagagt gacatggagt ttatagaaaa
    31261 agatgtaagc aataatgttg gagaagaaaa ttttcctaaa ttaataaaag tagactcatt
    31321 gagagaagga agtataaatg gagtaattag tatgtgttta aaagaaactg actatcaata
    31381 ttccttatct aataaaaaag gaggatatta atgtgaaata tttggtttca atagatggag
    31441 ggggaacaaa aactaaattt tgtgtaagtg acttagatgg aaacatactt aaagaacata
    31501 ccactggttc aactaattat aagtcagttg gaattaaaaa aacatatgaa aatattaata
    31561 atggatttaa aaaaatatta aaagatttat atatcgatta tgacgatatt gagtacactg
    31621 tatttggtat atctggttgt gattctccta atgattataa gattataatg gatgaaatat
    31681 taaaaattgg gattaacaaa gaaaaaatat atctggcaaa tgatgcagtt ttagcttttt
    31741 atgcacaagc tgattcccct ggcttagtaa ttgtggctgg gacaggttca attatactgg
    31801 gaattaaaga agatggagaa atatatagag ttggtggatg ggggtataat tttagtgatt
    31861 tgggttctgg atatgatata gggagaaaat tattaaagaa agtattgctt tattgtgatg
    31921 aatgtcatga gtatagtgat ttatttagtt gtgtcttgga cttctttgga gcaaatagtt
    31981 ttgaacaact tgcatatatg attactgata taaatgataa tgttgaaata gcaaacttag
    32041 cttcattagt gattgattgt gccgaacaag gtgataaatt ggctattgaa atattaagag
    32101 aatcatcaac tgaactatct aaattagctc aagtaatatt gagaaaaata tctaatccaa
    32161 tgaaaatgga taaaagcgaa ataaatatag ttttgtctgg tggtacatta aataaaactg
    32221 tatatgctca aatgttaatg aataatttaa aaacagaaaa tacagataaa aatttaaaat
    32281 ttatatctca agaaaatcag ccagtatatg gggggataag acttgctatg gcattagcta
    32341 ataggagggt caagcatgga tagaaaaaag gtaatagtac aaatttttac tggaggtttt
    32401 aaggctaaag aaataaagtt taatgatata aaatgtaaac tactagaatt attggactgt
    32461 attgatatag aaaaggttat catgggatgg agtgtagatg aagaattata tgtagaaact
    32521 aaaaaactat taaataaata taatgttgaa ctatatcttt ggttgccatg tttttctgaa
    32581 ataggattac ttgaaaaaag taatttacta attgattata tggggaagga agtcaagggc
    32641 tatgctttac aagaggatga aaattttgag ttttattgcc caaataataa ggaaaatata
    32701 aaaaatataa agaaaatata tagtacttat tttagtgata ttaaatttga tggaattttt
    32761 ttagataaaa taaggtatgg ttctttttcg aataaactaa atggtgtttt taattgtttt
    32821 tgtaaagatt gtttaaatat atataaaaaa aataatattg ataaagagtc gttaatgaga
    32881 gaaatgaata atgtgtctaa gggagttata gataatgaag atactccatt tggtataaca
    32941 gaatacatta atggaaaata caaatttaaa aataaaatat gggaagattt ttttgaaaca
    33001 aaaaatcaaa atatatacaa ggcattatta gagataagta gttattttaa aagtaaggga
    33061 ttgaaaatag gtatagatgc tttttctcct tttatttctt actttacagg tcaagattta
    33121 gaaaaattac aagaagtagc agattttata aaaccaatga tgtatagaac tacaaaagct
    33181 ccagctggtt taccatttga atttgaaagg tttattgatg aatccataca taagaataaa
    33241 gagattggaa aaagaaattt cttagatatt ttaggaatta aggagtatga aggagatgta
    33301 tatcctatag aatttgtact agaggaaatc gattttatga caagaaatct taataaagat
    33361 atatatgtag gagttgaaat aaataaaaag gaaattatat ctcctgttac acctaagtac
    33421 ataagtgaaa atatttcaaa tataaataag acaacagtaa aaggatatgt tttatcttgg
    33481 gatttgttgt cagcaccaaa agaaaatatt gatgaaatta taaaatggtt tcggaattag
    33541 tgaggtgata aagtgagtaa gatattggaa atacaacaaa tatataaaag ttttggaaag
    33601 gtagatgttt taaaagggat aaattttgag ttagaaaagg gagaagtaag agctcttttg
    33661 ggagctaatg gagcaggaaa atctacactt attaaaattt taggaggagt tcattctcaa
    33721 actaaaggta aagtaatttt gactggtaat gaaataatgt ttaaaaatcc acatcattct
    33781 aaagaaaatg gtattagtat aatttatcaa gaattaagct tgataccaac gcttacagta
    33841 cttgaaaata tttttcttgg tagagaaatt ttaaaaggaa gtgtgttttt aaacaagaaa
    33901 gacatgcttg aggaatatat gaaaatttgt gaggattttg attttaatat agacccacat
    33961 atagtagttt ctaatttaag tgtagcaaac caacaaatgg ttgagataat gaaagctgtt
    34021 tcatgtaatt ctgaaattat cataatggat gaacctacaa catcacttac taataatgaa
    34081 aaagatgggc tatttaaaat aataagtaga ctaaagtcta aaggtaaaac aatagtctat
    34141 atttctcaca tattagagga agtttttaaa gtatgtgata gtgcaagtat aatgaaaaat
    34201 ggaattatga ttggaactta tgatgtagag gatttaacta aattaaagat aactcaactt
    34261 atgacaggaa atgataatca caaacttatt gagaagaagg attatacatt tgcaaaatat
    34321 gaagagccac cagtactaga agtaaagaat ataagctggt ctaacaaaat aaaaaatgtt
    34381 tcatttaaag tttatagagg cgaagttgta ggtcttgcag gtcttgtagg ttcaagaaga
    34441 actgaaatta taaatttgat atttggtata gataataaag atagtggaga tatttttatc
    34501 aatggtaaaa aagtagacat aacttctcct aaggttgcta taaaaaacaa aataggattg
    34561 attcctgaag atagaaaaaa tctaggatta attttagaac aagctatata cacaaactca
    34621 tcatcagtac aaattgataa attcaagaaa tttttgttta tagatagaaa taaagaaatt
    34681 gagttttcta aaaaaggtgt taaggaatta ggaataaaag ttagtgatgt aaagcaagaa
    34741 gtaaaagagc ttagtggtgg aaatcaacaa aaagtagtag tttctaagtg gttagagaaa
    34801 gatttagatt taataatata tgatgagccg acaaagggaa tagatattgc tgcaaaagaa
    34861 gatattttca atacaatcaa agaattttca agtaaaggaa taggagtaat atttatatct
    34921 tctgatttag aagaagtaat aagagtaagt gatagaattt tagtaactag agaaggttct
    34981 attgtagatg atttaaaaaa tgagaatata caggttcaag atataatgaa cagtatattt
    35041 aatgtataaa aaatgaaaat ggaggactga gatgaagaaa gcttttgaga agttaaattt
    35101 aagagattat ggagtattat taggtttttt agctttatgt atagctataa gtattgcaac
    35161 tccatcattt ttaggtaaac aaaatatact taatctttta cgacaatctt ctataattgg
    35221 aataatttca gcaggtatga cttttgttat aatatctgga aactttgata tatcagttgg
    35281 agcagtagca gctcttgcag gtgctataac aatgaaattt gcgactggtg gaactaattt
    35341 gtttgcatcc atgttcttag gtgtagcgat ttgtgcagta ataggattaa tcaatggtat
    35401 agtagttgca aagataaatg taccatcatt aatagccact atggctatgg tcactattgt
    35461 aagaggattg ctgttgatgc ttacaggagg atatccaatt actgaaaata taccaatact
    35521 tgattacatt ggaaatggat atttgtttaa tataccaata ccagtagtaa ttttctttat
    35581 agtggtgtta gcttcgttta ttgtacttaa taaaactaag tttggtagat atgtttattc
    35641 tgtaggaggg aatcaagaag caagtaaact taatggtata aatgtagatt ctcacaaagt
    35701 gaaagtgttt attattaatg ctgtacttgc aggaatagct ggaatcgtac taacaggaag
    35761 acttgggact gcaactgcga ttgcaggtga aggatatgat atggatgcaa ttgcatctgt
    35821 agtaatagga ggtacatcag tggcaggagg ttctggttct gtacttaaaa ctgttatagg
    35881 agtattactt atgagtgtta taaacaacag ttttaatctt cttggaatag atgtatattt
    35941 ccagtacata tttaaaggat taataatttt agcagcagtt ggatttgatt cttatagcaa
    36001 gaaaaaactt gcttcaagat aaaaaataag ggggattttt ttatgaaaaa attaatgaaa
    36061 catttagcat tgttgctatc acttgttatg atatttggtt tagttggttg ttcaaatggt
    36121 ggtgacaaag aaaaaaatgg agaaaagaaa atagcagtat tactaccagg ttcaacaggt
    36181 tactttgtag caactaaaca aggtatagat gcaaaatcta aagaattagg tgtaagcgtt
    36241 gaatatgcag atgctcaatg ggatgctagt aaacaattat cacaagctga agattttatg
    36301 gctaaagggg tagacatgat tgcattatgt ggagttgatt ctgctgtaag tgagagaata
    36361 gttaaagctg ctaatgattc agacgttcct atagtagcat ttacaaattc tataggaagt
    36421 aacccaactg gagaatttaa aggtttagtt acatatatag gacaaaatga ggaagaaaca
    36481 ggagcattaa ctggaaaaat tgctaaaaac ttattaggag aaactggtgg gaaagctgta
    36541 ttaatagaag gagtaccagg aactactcca caagtattaa gaaaaaaagg tcttgaaaaa
    36601 gaattaaaag atagtaatat agaaatagtt tataatcaaa cttcaaattg ggagaaagag
    36661 caagctttaa aagtaacaga agatttaatt caaaagaaaa ctgattttaa tgttataata
    36721 tgtcaagatg ataattctgc tacaggtgca ggtcaagctt taaaagatgc tggtcttaag
    36781 gataaagtta aagtcatagg tttgggtgga agtaaagatg gattaaaagc tataactgat
    36841 gggttaatag atggtacaac ttatatgtct gctgtagaag aaggtggatt agtcatagaa
    36901 aaggcatcta aattccttaa aggtgaaaaa atagaacctg ttacacaaat taaacaagtt
    36961 gaagttaaca aagataatat ttcagagttc aagggtgaat ggtaaattgt catagataat
    37021 aaataagatt tttagagtta ctttagaaac tatataaagt aactcttttt ttaatttaat
    37081 ttagtgataa attattgaat agtatttcta aacaaataaa aaattaatat aaacaaagac
    37141 ttaattatac taaattataa gtaaaactaa tataaactta tggaaaaaag gaaaacgtct
    37201 aaagtattgg aattactata gtgtaggtgt agtattaaaa aaacataaaa aaacataaaa
    37261 aaattgttga aaaaaatata gaaattttgt ataattaaag atttgaaaag tgcggtatac
    37321 aaagccaatt cttttgtata taatatatat aaattcagaa aaaggaaggg atatgtatgg
    37381 ccaggcggtt atttttagaa aggattggac ttatattttt aggttcagca atattagcat
    37441 ttggagttta taatttttat tatttaaaca acataacaga aggtggagtg ttaggtattt
    37501 tactactgtt aaaaaattta ttcaatatac aaccagcaat agcaaatgtt gtaatagatg
    37561 gtttactact tttagtagga tataaatttt tcggtaaaaa attccttata tattcaatag
    37621 tagcttctat aactttttca gtgttatatg acttatttga agctatagga cctctagtac
    37681 cacaatcaca aaatatgctt ttatcaacta ttttggcagg tgtaactgtt ggaactggtg
    37741 taggtatagt agtaaaagca ggttgtgcat caggaggaga cgatgcttta gcattagtaa
    37801 tttctaagac tacatctttg aacatagggc aagtttattt agcaacagat gttattgtat
    37861 tgttgttatc attattttat ctatcagcgt ttgatatctt ttattctttg attgctgtta
    37921 caattagtgg caaggtaatt gattttattt attatcatgg taaaagtttg gatatggata
    37981 taagtaatga tatagttcca gaatgttaat ataaattaaa taactttaga ttttattagg
    38041 ggtggcagat ttgccacctt ttttgtgtct aaatctttat agaaagtata atcccatcta
    38101 ggtttttgca aatctcaaat atagaattat gttggaatga ctgggaatac gattgctaga
    38161 tgtgcatttt aaaaacccaa ttttatattt atgaaatact aatcattaat ggatgtttct
    38221 taatcctatc tttataccta aaatgataag ataaatgaag tagcaataca aaactaaaat
    38281 ttgtaaaaga gattaaatta aatggaggag ctaaaatgtt ggatatagtt atgatagcat
    38341 tgttattcat aggatttgtt tccatgaagt tacttgctga ttggtgtggg aaacaaactg
    38401 aaaaaaaata atgtaatgta tggagggatt acctatgtgg tttctggcag ttgtaattat
    38461 aatgttaata atatatttgg tatatgcgct tattaatcct gaaaaatttt aaacagtata
    38521 aggctaataa gtaagttgaa ttggggggaa caatttttat ggtgcagata gtaatagtgt
    38581 tagcgatttt tatgatttta gtaataccta tgggcaagta tttatatcat atagctacta
    38641 atcaaaaaac atttggagat agactatttg ataaagtgga caattttata tataaggtat
    38701 gtagtataga taagaaaaaa gaaatgaact ggaaacagta tgcattagct ttattattta
    38761 caaatgcagt tatggtattt ataggatata tcatacttag aactcaaagt atgcatattt
    38821 ttaatccaag tggaattaaa agtatggaac aaggactatc atttaataca ataataagtt
    38881 ttatgacaaa tacaaatctt caacattatt caggtgaatc aggactttca tactttagtc
    38941 agatgacagt gataatatat atgatgttta catcagctgc aacaggatat gcggctgcta
    39001 tggcatttgt aagaggtcta gtcggtaaaa agaaaacatt gggaaatttt tatgtagatt
    39061 taattaggat aacaacaaga gtacttttac caggagcttt aattattggg cttatattag
    39121 ttacacaagg agtaccacaa acttttgctg ggacagaaac agtgactact atagaaggaa
    39181 agcttcaaga tatagctaga ggtcctgtgg cagctcttga atcaataaaa catttaggaa
    39241 caaatggtgg tggattcttt ggttcaaatt catcacaccc atttgaaaat ccaacaataa
    39301 tatcaaatat agtagaaata ttatctatga tgatattacc aggagcttgt gttgttgcat
    39361 ttggtcacat gataaagaat aaaaaacaag gttgggttgt gtttggcgca atgtcaatca
    39421 tatttttaat aggtctagta gtgtgcttta aagcagaaag tgcaggaaat cctattttat
    39481 cacaattagg gcttaatcaa agtatgggaa gtatggaagg taaggaagtt agatttggta
    39541 tagcacaatc atcattattc acaacagtga ctacttcctt tacgacaggt acagttaata
    39601 atatgcatga tacactgact cctttaggag gattagtacc acttttaaat atgatgctaa
    39661 atgtagtatt tggtggtaaa ggcgtaggtc tgatgaatat gttgatgtac gctataatag
    39721 cagtattctt atgtggatta atggtaggaa gaacaccaga gtttttaact aagaaaatcg
    39781 aaggaaaaga aatgaagctt attgctttac ttattatatt acatccactt ttaatactaa
    39841 tgttctctgg tctttcagta gcaataccag cagggcttga aggaatttca aatcctggat
    39901 tccatggatt atcacaggta ttgtatgagt ttgcatcttc agcagccaat aatggttcag
    39961 gatttgaagg tctaggagat aacactatgt tttggaatat aacaacaggt gtagttatgt
    40021 tctttggaag atatgtgtct attatagtac tactagcaat atcaagtttg ttagcatcta
    40081 aaaaagctgt aaatgaaagt ataggaacac ttagaacgga taattttaca tttacaatag
    40141 ttttagtact tgttgtttta attgttggtg ctttaacatt tttcccagcc ttagcgcttg
    40201 gacctatttc ggagcatcta gtgttgtggc actaaaaaat tgggagggag taaagtttta
    40261 tggaaactaa aaaaacaaag tttattacaa aagatatttt caaaacttcc attatagaag
    40321 cttttaaaaa attaaatcct aaatacatga tgaaaaatcc agttatgttt gttgtagaaa
    40381 ttggatttat agtaacattg atacttacaa tagtacctag tttgtttggt gatgaaggca
    40441 ataacttaag agcttataat gggattgtaa caataatctt atttataaca gtcctttttg
    40501 caaattttgc tgaagctgta gctgaaggaa gaggtaaagc tcaagcagat acacttaaga
    40561 aaactagaaa agatacagta gcaagggtta tagatacgtt aggaaatgaa aaaatgataa
    40621 gtgcaagtga gctaaaactt ggtgatattg ttttagtaaa tgctggagaa gttattccaa
    40681 atgatggaga agttatagag ggtatagctt ctgtggatga gtcagcaata actggagaat
    40741 ctgcacctgt catgagagaa tcaggtggag atttcgcatc tgttacaggt ggtactacag
    40801 ttgtaagtga ctggttaaag ataagaataa caacaaaacc aggagaatcg ttcttagata
    40861 aaatgatagc tcttgtagaa ggtgcttcaa gacaaaaaac gccaaatgag attgctttaa
    40921 atactttatt agtagggtta acacttatat ttatggttgt tttaatatct ctttatccaa
    40981 tggcaaacta tgcaggtgta aagattccaa tttctactat gatagctctt ttggtatgtt
    41041 taataccaac tactataggt ggattgttgt cagctattgg tattgcaggt atggatagag
    41101 taactagatt taatgtaatt gcaatgtcag gaaaagcagt agaagcttgt ggagacgttg
    41161 atactatgat tttggataag acaggtacga ttacttatgg gaatagactt gcatcagaat
    41221 ttatagcagt tggaaatgca gattcaaaag atttaataaa ttattctgtt atgtgttcat
    41281 taaaagatga tacaccagaa ggaaaatcta tagttgattt aggtatgaaa ttaggttcaa
    41341 caggtaagac aaaagaggca gaggaagctg agtttgtaga attttctgca caaactagaa
    41401 tgagtggtat taacttagct gatggtagag ctataagaaa aggtgcttat gatagcataa
    41461 taaaaagagt aaaagaatct ggtggtacag tgccagaaga tttagaagaa aatgtaaatc
    41521 gagtagctaa actaggagga acacctttag tagtgtgtgt agataatgag atatatggaa
    41581 ttatctactt aaaagataca gtaaaatcag ggcttgtaga aagatttgct agacttcgag
    41641 agataggaat aaagacaata atgtgtacag gggataatcc cttgacagca gctacaattg
    41701 caagagaagc tggagtagat ggatttattg cagaatgtaa accagaagat aaaatagatg
    41761 caataaaaaa agaacaatta gagggtaaaa tagttgcaat gacaggtgat ggaacaaatg
    41821 atgctcctgc acttgctcag gcagatgtag gtcttgcaat gaatagtggt acaacatctg
    41881 caaaagaagc agcaaatatg gtagatttag attcagaccc aactaagata ttggaagttg
    41941 ttgaaatagg aaaacagctt ttaataactc gtggggcttt gacaacattt agtatagcaa
    42001 atgatgttgc aaaatatttt gcgattatac cagctatatt tactcttgca atacctcaaa
    42061 tggagatatt aaatgtaatg aaactttcta cgtcatttag tgcgatatta tcagctttaa
    42121 tatttaatgc tataattatc ccatgtttaa tacctcttgc aatgaaggga gtaaaatata
    42181 gacctatgaa atcacaagct atgttaatga gaaatatgct tatatttggt cttggtggag
    42241 ttatagtacc atttttagga ataaaattaa ttgatttatt gattacacct ttagtaatgg
    42301 ttcttaattt aggttaataa atggaggata taaaaatgaa aacattaaag ccagcattac
    42361 ttgtaagtat tgtattgtta gttgtttgtg gacttgtata tccacttgtt ttgacaggtg
    42421 taagtcaagt tgcatttaaa gataaagcca atggaagtat gatagaagta aatggtgtaa
    42481 aagtaggttc agaacttatt ggtcaaagct ttacagatgc gagattcttt aagggaagag
    42541 tatcatcagt aaactataat acttatacaa aagaagattt agtaccagat aaagatggaa
    42601 atacatctta tggaggtgta tcatcagggt cttttaatta tggagctaca aatccagaac
    42661 ttcatgatag agtacaaaaa gatatagaaa agtttttaaa ggataatcca actgtcaaaa
    42721 gagaggatat accaacagat ttacttacag catcaggttc aggacttgac cctaatataa
    42781 gtccagcctc tgcaaaaatt cagataccag ctatagcaaa agcgtctgga atatcagaaa
    42841 gtaaattaca aaaaattgta gatgataata cttccaaaaa actttttgga gttttaggtg
    42901 aagatagagt taatgtatta aaagttaatg tagaggtcgc aaaaatatta ggtcttatat
    42961 agtattttgt ggaattttag caacaaaata ttacgcaaga tttaatttaa aggttaaatt
    43021 ttaaagagtg aaaatcgata atgattttca cttttttatt tgaatattac taatatttgg
    43081 aataaagtga ttgtaagtat aaaatccaaa aaaaggttgc attatcaaaa tgataatact
    43141 ataatatatt tatcgttatc aatttgataa tagatttgga catataggag gaaatagtat
    43201 gttatataat aacgcattag agttgatagg aaacacacct gtagtaaggc tgaataattt
    43261 aggctgtact aatgggtgtt ctaacatata tgtaaaatta gaaaaagtaa atccagcagg
    43321 aagtataaaa gatagagctg tttatgaaat gttagagggc ttagagcaaa gaggagaatt
    43381 aaaaaaggga gatgtactag ttgaagcaac tagtggaaat actggtattg cattatctat
    43441 gataggaaaa ttaaaaggat atgaagtagt aatcgtaatg cccgaaacaa tgagcgttga
    43501 gagaagagat ttaatgaagg cttatggagc aaatttggtg cttacagatg gtaaagctgg
    43561 aatggcaggt tccatagaaa aagcaaatga acttttaaaa gaaaatccaa actataaaag
    43621 cctaaaacaa tttgaaaata aagataatcc aaatgcacat tataaaacaa cagcagttga
    43681 aattatgaat gatgtcaaag atttagatat attcgtatgt ggagttggaa caggtggtac
    43741 tttaatagga actgcaagat atttaaaaga acaaaatcca aatataagag taattgcact
    43801 tgaaccaaaa aaatctccag ctatatcaga aaacaagact ggacctcata aaatacaagg
    43861 aataggtgct aactttgttc ctgaaaatta tgattctagt gtagttgatg aagtaatatt
    43921 agtagatgat gaagatgcat ttgatacagt taaattactt gcagcaaaag agggaatttt
    43981 agtagggata tcttctggag caaatatatt tggtgctata gagatggcaa aaaagtatcc
    44041 agataaaaag atagttacag tagctccaga tggagttgat aagtatatgt ctatgggaat
    44101 attttaataa ttttatttga gggagtgaat actttgttta agaaaataaa taaagatatt
    44161 gaatatataa tgaagaatga tccagcagct aggtctaaga tagaagtttt tttactttat
    44221 ccatcagttc atgcaatgat aatgcatagg atggctcatg ctctttataa gaagaagaag
    44281 ctttttacag caagattaat atcccagata tccagattta tgactggcat agaaatacat
    44341 cctggtgcta aaatggggga aggtatattg atagaccatg gaatgggtgt tgtaattgga
    44401 gagacagctg aagtagggaa tagggtgaca atatatcaag gagctacttt aggagctaca
    44461 ggaaaagata ctggaaagag acatccaact gttggtgatg atgtattaat tggtgctgga
    44521 acaaaaatac taggaccttt aaatataggt tcaaattcaa aaattggtgc aaattctgtt
    44581 gtcgttaagg atgtacctaa tggtgcaaca gttgttggta taccagcaaa aatagtaaaa
    44641 ataagaaacc tagaacctgt aaaaaagaat aaaaaagaag tgtcctatga gtatgatgaa
    44701 ttagataatg tatattatat ataggattta tataactata aaataaactt tataaaaatt
    44761 attttaaaag gtattttttt caaatttgag gacataataa aaaagaactg aatgttacag
    44821 ttttggttca tcaatctttg ataaacaaga ttttaaattt gtagcgtcta ggaggatttt
    44881 attatgaaag caaatgttaa tcaagatact tgtataggtt gtggattatg tccatcaata
    44941 tgtccagaag tatttgatat gaaggatgat gggaaatcac atgttatagt tgatgaagta
    45001 ccaagtgatg ctgaagaatc agcagcagaa gctagagaaa gctgtccagt tgatgcaata
    45061 gatgtgcatt aataaaataa atgcctataa aaattgaatt ttcaattttt ataggcattt
    45121 gtcttttatt cagttagtat ttcaacacct gtttctgtaa ctaaaaccat acattcccat
    45181 tgagcagata atttaccatc ttttgtataa atagtccaat tattttcttc atcaacaaaa
    45241 atttcgtgag ttccttcatt taccataggt tctattgtga atatcatccc aggaaccaat
    45301 agcatctcag tccctttttt agatacataa cttacaaaag ggtcttcatg aaagcttaat
    45361 ccaactccat gaccacctat ttctctaaca acagagtatc catttttctt tgcataagag
    45421 ttgatagcat cacctatatc tcctaaaaat ccccatggtt tggcaacttc aagacctttt
    45481 tggacacatt cctttgtaac ttgaactaat ttttttgcat tttcattaac attacctatc
    45541 ataaacattc ttgaagcatc tgaatagtat ccattaaaaa tagtagaaac atctacattt
    45601 ataatatctc catcttttaa gataatgtct ttacttggaa taccatgaca aacttcatta
    45661 tttactgatg tacatacact tttaggaaat ccattatagt ctagtggagc agggatagct
    45721 ccattttgta gtgtaaagtc atgaaccaac ttatttatat cttctgtaga cattccaatt
    45781 tcgattttat ctgaaatata atccaaaatt tttgtgttta ttacagcact tttttttatt
    45841 tcctcaattt gactttcatt ttttataata tcatgagttg gtacaatatg acctaactta
    45901 gcaaatgaat ctattttatt atccatatcc atatgacatt ttttatattt tttagtactt
    45961 ccacaccaac agctatcatt tctatttaac ttcataactt ttcatccttc ttttattatt
    46021 tatttaaatt atgggttaca taaactattt taaaacataa ccattataaa tattataaca
    46081 taaatactaa gaaatccttt atagatagtg atttattcaa agtataaatt attatatggt
    46141 tattgttgat gaaaagagga tagttaaatg tattattgga tattagcgca cttaatagtt
    46201 tagttcaagt ttttcaagaa aatcatatgt tagaggacaa ttttccttta aagtattttt
    46261 tgaatcttca tcaagataat aatgtacaaa tgattctgca aaaaattctt ctataaactt
    46321 gcttatatat gcatgtgcaa cttgcttagg gtcataaagc ttatatcttt cctgagcaaa
    46381 tacttccata aattcttcag aatttgattt tttatttaaa acatttttat ctatagcatg
    46441 tgcagtttca tgtaattcta aattttttga tgaatgtaca tttgcatagc tagggttact
    46501 ataacctatt ttagccacta tagaattatt tctatatata ccaggtacat ctttccaagt
    46561 cttgcctgat cttacccaac atggaggtaa ttgatatttt aaatcactaa attctggttc
    46621 atcagttaga tttgaattta ttaattttat ttttatatcc ttttctttaa gtgtctgtaa
    46681 atatttaata tttatttttg caagtctgtt tttcatattt tcaacttcat ctttatcata
    46741 attcaagtca tcaggaagta taagcatttg ttcaattata tctatactac tgtcttttat
    46801 attttcagat tcttcatcaa acttatattg aagagtatta ttttcataga tagttatatt
    46861 tgtattattt tctatagaat aagagtgaat atcctctaaa tctaaaatat tcatttgttg
    46921 taaaatatgt tttgtttcgc cactccatac tactaaagaa agtgaattaa aattaattag
    46981 tggattttct tttgctttta tatcattgct tatatctaca tcaaatacat attcaattga
    47041 gtcttctatg ttaagaccat ctgtattctt tttaatatat aaatacgctt gttttaagtc
    47101 tatataaatc tcatctaaag attcgtcata aaattctgat aaattattag taaagagttc
    47161 ttcacttaaa agttcatttt tatcagttat actttcaatt aaaatattaa tttcttttat
    47221 catatcgtaa atacttattt ttctatttgc tttttcacta gatacattgc atggcgcaag
    47281 agcaatgcaa taaaaatctt tttctttttt ataaaaggct ttaagtgatt ttgataaaag
    47341 tgtaaagtct acacctatct gtaagttgac cttaagtgag tcattttgta ataattcagt
    47401 attatttctt ttagctgtat ttaaattgtt ttccataatc attgccccct ttttattagg
    47461 gtatcataat ttttttatat gtgtcaattt gtttttttga aaattataat ttttttgaaa
    47521 attttatttt ttataatttg tgaatttttt attatagaaa taaatatgtt attattatgt
    47581 caaaatttag cattagattc atgtagagta tgttttatac atatattata attaattctt
    47641 tgaagattgc atattcaaaa tacacaccat tttttttaat tctataatat aatatttata
    47701 tatttaatgt taggatgtga tttaaggtgt attttacata tattataaga tgtaaagatg
    47761 attctctata cactggatat accagtaata tagttagaag aatgaatgaa cataaattag
    47821 gaattaattc aaaatacaca agagcaaagg gatttgaaaa actagaagtt tattttgtga
    47881 ccaacacaaa aagcaatgct atgaagttag aatactatat aaaaaaatta actagaaata
    47941 aaaaattgtc tataataaaa aacccaagta tacttataaa tttaattgat aataaagaag
    48001 attatattat tggtaaggaa atagaacaat taacataaat atttatagta acattataat
    48061 gggtgcatat ggtgtaaaaa cttgacaata aatactattg taatataatg aagttataaa
    48121 tatagtaaat taaaatagct aggggagcca agagctgaga ggtataccga cccttacacc
    48181 tgatctggat aataccagcg taggaaagct tagtaaaaat tcaagtatgt attatttgtg
    48241 atatatgcta tttctaagct atacctattg taggtatagc ttttttaatt aagttattta
    48301 aattaaactt taggaggcaa caatgagtaa ttataaaata ccaacactta caatagccgg
    48361 ctctgattca tctggaggag ctgggattca ggctgatttg aaaacgttta gtgcaattgg
    48421 tacttatggt atgagtgtaa taacagctat aacagcacag aatactaaag gagtttttgc
    48481 tgtagaggat ttaaataaga aaataataaa aaaacaaata gaggcagtct ttgaagatat
    48541 acctccaaga gctgtaaaaa taggaatggt ttcaagtcct gagattatac ttgaaatagt
    48601 tgagaatcta aagaaataca atccaaaata tttggttgtg gacccagtaa tgatatctaa
    48661 aagtggatat tatctattaa agccagaggc aaaagaaaac ttaattaagt atttgatacc
    48721 acttgcatac ataattacac caaatatacc agaggcagaa gaaataacag gaataaaaat
    48781 acataatgta gatgatatga agagagtagg agaagaaata ctacaacttg gacctaaatt
    48841 tgtacttatg aaaggaggtc atttagatgg agaagcagtg gatattttag ttggtaaaaa
    48901 tatatttaaa gtttataaaa gtgaaagaat tgataaaaaa aatacacatg gaacagggtg
    48961 cacattatct tctgcaataa catcttatct agcattagga tatgaaataa cagaagcagt
    49021 aaatctaagt aaaatctata taacagaagc aataaaaaga agttttgata taggtcatgg
    49081 agtaggacca gtacatcatt tttataaatt tgagtaataa aaaaatattg gaggtagaat
    49141 atgtataatt taattaaaga tgtaaaaaaa ttaaatccat tggtaattca ttatactaat
    49201 aatgtaacta taaatgattg tgctaatgta actttagctg taggagcaag tcctcttatg
    49261 agtttttcat atgaggaagt agaagaaatg gtaagtgtag caaactctgt agtaataaat
    49321 ataggaacta tgaactcaaa tatgttggat ttattcctat tagctggaaa agcagcaaat
    49381 aagtacaata aacctgttgt tttggaccca gttggagtat ttgcaagcaa agcgagagca
    49441 gaacttacaa gtagactttt aaatgaagtc aaatttagtg ttgttaaagg gaatgtatca
    49501 gaaataaagt ttataggtgg ctttaatgta aggggtaaag gtgtagattc atttgatgaa
    49561 gaagaagatt ctactgagat aataagaaag attgctgaaa agcttgaatg tgtagttgta
    49621 gcaactggaa aaatagatat aataactaat ggtaaaggta catataaaat aaacaatgga
    49681 actgataaat taaaaggaat tacaggaact ggatgtatga ctgctagctt gattgcaagt
    49741 tttatggctg ttactgagaa tatattagaa gcagcaacaa tgggtgttct tacaatgtct
    49801 ttaagtgggg aattagctaa tttaaataat ccaccaatag gtacatttaa agaaaatctt
    49861 atgaatgcta tttatcaaat ggatatagat gctttaagta aaaattctaa tattgaattt
    49921 ttaaattaat aaagagtaag ggagacttaa aatgattgat aaagaatctt taaaaaaatg
    49981 tttaaagttg tatttagtaa ctgactctga aatgcttaaa ggtagagact tttataaatg
    50041 tcttgaagat gcaatatctt ctggaataac aactgtacaa cttagagaaa aaaatgcttc
    50101 tggaagagag tttttaagaa aagctatgaa acttagagaa ataactaaaa gatatggtgt
    50161 taagtttata ataaatgata gagtggatat agcacttatt tgtgatgcag atggagtaca
    50221 tgtaggacag agtgacattg atgttagaga ggttagaaag cttataggaa acaataaaat
    50281 tctaggagta tcagcaagaa ctttagaaga agctatttgt gcaaaaaatg atggcgcaga
    50341 ctatttaggt gtagggtcta tattcacaac atctactaaa ttagatgcaa aatcagcttc
    50401 gtttgaaact gttaaagaaa ttaaagaaaa agtagacatg ccatttgtac taattggagg
    50461 tataaattta gacaatatag ataaacttaa atgtttagaa agtgacgggt atgctataat
    50521 atcagctata ttaaaagctg aagatatctc taaagaagtg gaaaagtgga cattaaaaat
    50581 ataaaattgg ctctggtagt gaaaaaaatg gaaaaatttg atataatcta agagtaaaat
    50641 ttaagcaatt ataaatgata aacttttcta catatacttt ttaatcaaga tatatgtaga
    50701 tttggttaaa aaggagctat aaatgattag gatattgtta gtgtgtgttg gaggcatgtc
    50761 ttctacccta ttggtaaata aaatggagaa ggatgcaaaa aaaagaaata tagactgtaa
    50821 aatatgggca gttggagaag gagatataaa atcggagctt gataactttg atattttact
    50881 tcttggacca caacttagat ttatgcttga tgacgtaaag tctatagttg gagatagagc
    50941 tccagtatct ataatagata tggtaaacta tggcacttgt aatggtcatg ctgtgttaaa
    51001 ttcggtttta gaaatattaa aataaataat aaaagtggat tctatataat agagtccatt
    51061 tttattattt gttattaata ggcttaaaac ttagcttacc atttcttttt ttaatctcca
    51121 catagcaagt gaaaaacttg aacagcacaa tattaaaaat agtaggataa caggcattat
    51181 atctccaata agaatagtct caccaaatat taagctacgt aaagcgttaa taacatgggt
    51241 aaatggattg agcatcactg ctacttttaa tcctccagat aaactttcta tagggaaaag
    51301 agctgaactt aaaaagaaaa taggaagtac aattgcagtc ataattgttt cataaactat
    51361 ttcatttggc aataataaac ttatagaata agctagactt gataaaaaga aggctgtcat
    51421 aaatatcagt actatcataa gtaggattcc accaatgcca gattctatcc ttactgagaa
    51481 gaatatactt actatacaca aaatagttac ttctatgaaa gaaactaaga tagcttctaa
    51541 taattgacca aggacgattg aatacctgct gataggtgca attaagactc tatagaaact
    51601 tccactgttc ttcattataa agtttataat accaccacta ctacaagaag aaaacacaac
    51661 cagtacaatg atacctggta gtataaaagc agtataatta ttgatattta tattattcat
    51721 agtttggttt gcaatagaac tataaagcac aagccaaaga aaaggttgta atatagtaac
    51781 tacaaatgag attggatttt taaaacgcca cttcatacta cgccataaga tatttaacat
    51841 tcccattcct cctttaagct ttttttagaa cttgtaagtg ctaaaaatac atcttcgaga
    51901 cttggctcta caatttctat tgcatcaaat tctatatcat gttctaaaag ccatttattt
    51961 attaaagtaa aagcagttct actatcgttt acacttataa aaattgaatt ttctcgtacg
    52021 ctcataaact ttactaaacc agtactttta attgaatctt tatatttttt tatatcttca
    52081 gtattatgaa aagttatacg tagtatattt tgtctaatgt aactacgtaa acttgatggt
    52141 gttccttgtg ctaaatcctt tccattcttc ataatacaaa tattatcact caattgttca
    52201 gcttcttcaa gataatgagt agttagaaat atagttgtgc catactcatc tctaattttt
    52261 aataacatat cccacataga ttttcttgaa tcgacatcca taccaacagt tggttcatct
    52321 aaaaatagga ttttaggaga tgataccata ttcatagcta tgtccaatct acgttttaca
    52381 ccccctgaat atgatgaagt aggatatttt aagtaactag ataagtcaaa cttatcaatt
    52441 aaagagtcta ttctttgttt tgcaatctgt ggttctactt tatacatttt actttgaaat
    52501 accatatttt ccataaggga taaatgttca tcaatagaga tttgctgtgc tacacaagca
    52561 atttgagtac gtatccaaga aggattatct actaaatctt ttccaaacat tgttacattt
    52621 ccagatgtag gtttgtaaaa ggtagtcaaa atatttataa gagaagattt accagcacca
    52681 ttaggaccaa gtaaagaaaa aatttctcca gcatttacat taaagcttag accatttaaa
    52741 gctttcacac catttttata ttcttttact aaattgtcta tttcaatcgc taacatatat
    52801 tattcctcct ctttaagtgg aaatattatc tctgtaatat agttatctgg gtttcctttt
    52861 atcaacatac ctggtccttt aataaatact tctctccaag gagtttgtat ttttatatta
    52921 ttttcatgaa tatatttaaa tatcatttca tacgtttttg gcaaattact ataagagcct
    52981 gtgtgagtaa aaaataaagc ttttgtttta ggaaattctt ttgtagttat acccatacga
    53041 tttggagttt cagcagttgg aacacataat tccatttctg caacaccagt tttttggtca
    53101 acatcatagt agcaaaagaa tggtgcaccg ttagactttc cttttatagc tttaaataca
    53161 tttggaaaat actttgttgc ctcagtcata ggtccattat aacgcatggt agccactgtg
    53221 actgattcga tatttttgat ttcaattttg caattcattt gtttacctcc taatgtgtgt
    53281 tcatattctt actagatttt taagcattaa tacatataag attttgatta gttgaaaaca
    53341 tataatctta tctcgtttgc ttgttttcaa gtctaacata acataaaata tatatcctct
    53401 catttgatgc tccattttgt aaagttatta tttaagcttt actttagatt ttttagcaaa
    53461 ttaaaataaa gcaatttata aaataagtat ttacaaatta agtgtattaa tcgtataata
    53521 cacttaatag gatactagat aaagtctgaa ataaaggcgt atttttttaa agtaagtgta
    53581 ctatcgtatt aatacactag aataggagga atcatgaatt ttgaattaga taatacaact
    53641 cccatttatt tgcaaatagt aaagtatata aagagacaaa tagtaacagg agagttgaaa
    53701 ccaggagaga caataccatc aagaagagaa atggcactta atctaaaagt aaacttaaat
    53761 acagttcaaa gagcatataa ggagatgggt gatatgaata taataaatac ttttaaaaat
    53821 tatcaaagca gtgtaacagt tgatgaaaat atattaaaaa atttaaaatt agagcttata
    53881 aatgaatcac tatctgtttt tatagaagat atgaaagcaa taaatgttag caaagaagaa
    53941 gttttaaaaa taatagaaga taagtattag ttttttaaca aatagaaaac ttagtttaga
    54001 aagtttattt ataagcatat tggatgtgga ggatagtaaa tatgatagaa ataaagaatg
    54061 tcagcaaaac atataaaagg atgcaagggc ttaaaataag aaagatagaa gctttaaaaa
    54121 atgtaagttt taatatagaa aagggaaaaa taactgcatt acttggtata aatggtgttg
    54181 gtaaatcaac tatgttaaaa gcaatagcag gacttataaa cattgatagt ggagaaattc
    54241 gtatagatgg agaaaaaata aatgaaaagg tatacaataa attggctttt gttccagatg
    54301 tacaatctca ctttagcaat acaacaataa aagaaacatt tgaatttatg gaaatttttt
    54361 attcaaagtg gaataaagaa aaatcaaaag aaatgatgga catatttaaa ttggatgaag
    54421 atgaaataat tgataacttg tcaaaaggaa acattgcaag agtaaaatta atacttggat
    54481 tttgccaaga cccagagtac atacttcttg atgaaccatt tacaggaatt gatttgttta
    54541 agagggaaga gtttatagga gtaatagcgc aatatatgga ggaaaatcaa gccattatta
    54601 taactactca tgagatagta gagatagaaa gtttagttga tgaggttgta attcttgatg
    54661 aagggcagat tataacttca tttaatgcag aagaattaag agaaagagaa ggtaaatcta
    54721 tattagataa gatgagggag gtatataaga atgaataaac ttctatatct aataaatgta
    54781 gatttgagaa gaagcaataa gttttatata gcttatatat catttttttc attaattaca
    54841 cttagtttaa atttattcga aattaataaa tttaaaaatt ctaaatttat tatgaactta
    54901 gcaatagaag attttggagg tatattttat ggactaggga tacttagtaa tcttggtttt
    54961 atccaaagta tattgttctt tgggatattg ggacttttta tatactatat ttttatgtgg
    55021 aaaagagagt tcttttcaaa taatcaaagt atttatacac ttatgatgtt acctcaaaat
    55081 aagtttaaga tttgtatatc taaatcaata gccttgataa caatgatata tggttttttg
    55141 ataagtcaga cagcaacatt atttatatgt aaatatatat ttaatttcat ttttagaaat
    55201 atgcctataa tcaatatgaa ctttgccaaa gatttatatt atttaaactt gaagagtata
    55261 ccaattgatt ttatctcttt tatggctatt catgtatttt tactacttat agctatttct
    55321 attgtaaatt gctgtatatt atttatcttt tcctatataa ataaatttta tgtaatttta
    55381 atcactttgc tgattatagc aggcttatat ttttcaacaa tatatataaa tataatcact
    55441 ttatttagaa aatattataa tcttatagga tttcaattta acaatttagt agcagggtta
    55501 ttaagttttt taattataat gtttattcta aatggaatat cttatttgct aattaaaaag
    55561 aaagtatctc tttaaggagg ggtggtatga aattaaaatc taaaatatct atatttatta
    55621 tatttttgac tttagcaata tattttagta taggtttatt aaataatgaa gataatttaa
    55681 agataaaaac tataaaaggt gactctaatg aattagacca tataaacata aattttggat
    55741 atgctttgaa tggatatata aattcatatt atacaataaa tggaagcaac aaaaccaaaa
    55801 agactgaatt aataaaacta caaaaggata gtaagaacac aaataattta attgatgtat
    55861 ctagtggttc agatttaatt gctcatgttg gatttgaaat gaatgaggag tcaaaagata
    55921 taaactttgt gatagatgag aaaaaagaag atttagaaaa aggtgtattt ccatcagaag
    55981 gtagtgataa agaaattgaa tatgaaaaat tagatatgaa taattttaca aggacaagat
    56041 ataaagtaga taaaagtaca gatgattatt atgcaaagat actagcatca aaaagatata
    56101 aaaaatactt atatgtatct gtactttatg ctacaagtga ttcatcagaa aataacaaag
    56161 aatattcaag tactatagat ataataaaaa ttaatccaga aaataaaaaa atcaagacta
    56221 taaaaagtat aaatttaagt aattattttt ccgaaaaata ccaaaacata gagaacatag
    56281 gtgtaagtta tgctagcaaa tatgaggata agatgtatat tttagtaaat gtactaaata
    56341 caaacatagg tgataatagt aattctaaat ttcatttact tgagtataat cttgaagaaa
    56401 ataaggaaag cataatggaa ttaaaaactg atagcaaaat ggctttagca aaagcaaagt
    56461 tagaagctaa taaattatat atggtaatgt cgaatgatta tgataatagt tcaattacag
    56521 agttaaattt acttacctat aatatattag ataaaagctt agatgaagat aaatatgttt
    56581 tagataatag agaaaaaaga ggaattagaa gtattgaaat tgattcaaat aaaatcgttg
    56641 catatacatc aaggctaaac tctatattat cttgggatga taaatttatg ctatatataa
    56701 taaataaaaa ttctaaggaa attgtatatg aaggagaatt acaatttaac agttcaaata
    56761 gtatgttaga atttgaaata aagtaggtga gttgatgaaa gatttaataa gattattcga
    56821 atttgaatta aaaagaaata taaagaatta tatatttata ataatttgtt gttgttcttt
    56881 tgtaatttta aacatagtaa aaaatttaaa tgactataat tacataatag aaaatgcagt
    56941 taaaagtgaa cagttaacaa aaatcggaaa tacgatagaa acggtaaatg ttacaggttt
    57001 ctcaagcttt agaaatataa tgggttctac agaatcgtgg tttatgtttg gtataattgc
    57061 atgtatttgc tatgcttttt ttatttggta tagagacttt aatggtagaa gtaaatctat
    57121 atatacactt ataatgctac ctaaaaatag aatcaatatt tatatatcta aattattaaa
    57181 tatattattt ctagtgtata gctatacagt agtacttaca atatctttgt ttattgcaag
    57241 taagttgttg cctagacata tgcttggtaa tgtcacaaac tatggttttg tacaagaaac
    57301 aatatatgag ttaaaaatgt tacttccata tagctttgaa atactatttg tagagtatat
    57361 atttttattg ataggatttg tatctgtagt atttacatca attcttataa ataaatctat
    57421 ttataaagta agcactttaa taagttttct atttttagtt atagaaattt tagtgtttat
    57481 aatttctata gaatttatta taccttacga atacagtgat gtatttactg ttctatattc
    57541 aagtataaac atatttgtat gtagtcttat atctaataaa cttttgaagc aaaagattga
    57601 cttttaggag ggatattatg aaagcaaaaa atataggaaa atatacaata tgttttataa
    57661 tagtatgttt tttaatgatt actatgacct taatattgag ttattttaat aaggataaac
    57721 tatctgtaaa aactttaaaa ggcaatgtaa aagatatagg gagagtaagt gtagtatata
    57781 cacctaatat aagcaaattt tcttatgagg aggtaattat aagtaaaaat ggagtaagaa
    57841 ataatacaaa cccaaaatca agaattagaa atgattattt aaatattgac aaaaaataca
    57901 gtgactttgt taatgataaa ggaaaagaat ttatatataa agatgatttg aatataggag
    57961 aagcgtatac ctctgttaag tctgatttag tacaaggaat gaatgcttta aatgattgta
    58021 taaaggtttc aaataaaaat ttaaaaagtg gggtagaaga aaattttgat attaatttaa
    58081 ataagtctga ttttaaagaa aatagttgga aaaccttatt taacaaaata tataaaggta
    58141 atttatatat ggtaattagt gaaactaata tttcaggttc taaagtcaat aatatttgta
    58201 tattaaagat agatataaaa aataagtctt ataaattatt aaatagattt aatttaggat
    58261 tacagaatgg agaacaacaa aatatcaatg aaattaaatt ttctattgga agtaaattat
    58321 acttagaaat agtaaataat aaaagtaaaa atggatatat tgaaggacat gatagttact
    58381 ctaactttat tatatatgat atagaagata acacttttaa tataagtaat aaatttatgg
    58441 ataatctaaa caaagaaaag aatgcaaatc caaaggagat tggcttatta agtaaaacta
    58501 agctagatta tatggtaaaa aacaataaat tgaatcttat agttacggat atgagtaatt
    58561 ccataactaa tcttgtttat gatattaatt ctaatgagat taaattagaa gattatgaga
    58621 tacttgattt aaaagtagat tataatgaag catcatctgg agttaggaaa gttaagctac
    58681 tggatgataa gatatattct atttcaggta ttaagaacag tatatcagga gaattaaaag
    58741 gatttaaagg gattattgat gtagtcggaa ctaagccagt aaaatttcaa gtatttgata
    58801 tgaaacttaa aaagaatatt tatgaagctg agctaataaa tggagatata agtttacaag
    58861 aaaagttatt ttttgttgca aattaagtgt tagaattaat tttagaactt taaactttta
    58921 tatgtatcac agataagtat aagtaaggga gtgtctcaaa ataaactttt tagctcatga
    58981 gacacttttt ttatatgaaa tcaaatatat tttcattgtt tagatttatt ttg
//
LOCUS       DAYAPA010000008        57564 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.008, whole genome shotgun sequence.
ACCESSION   DAYAPA010000008 DAYAPA010000000
VERSION     DAYAPA010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 57564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57564
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.008"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            186..1382
                     /locus_tag="ACGI59_000413"
     CDS             186..1382
                     /locus_tag="ACGI59_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HFG9738226.1"
                     /translation="MKILVLNCGSSSLKYQLIDMNNEEVLCIGLVERIGIEGSILKHE
                     KAGRDDKYVVEQPMKDHKDAIALVLEAVAHPEFGAVKEMKEIDAVGHRVVHAGEKFAT
                     SVVITPEVEEALKECIDLAPLHNPANIMGIDACKAILPDVPMVGVFDTAFHQTMPKSS
                     YLYGLPHELYTKYGVRRYGFHGTSHNYVSQRAAEILGKDIKDLKIVTCHLGNGASIAA
                     VDGGKCVDTSMGFTPLEGLIMGTRCGDIDPAILPFLMRKEGLDADGLDKLMNKESGVY
                     GMTGISSDFRDIEDAAKNGDERAQATLEAYVKKVQKYIGAYAAEMNGLDVVVFTAGVG
                     ENGKAIRADIASNMEFLGMKLDKEANDVRGKETVISTADSKVKMLLIPTNEELMIARD
                     TLRLVK"
     gene            1475..1999
                     /locus_tag="ACGI59_000414"
     CDS             1475..1999
                     /locus_tag="ACGI59_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HFG9738227.1"
                     /translation="MKVSIEKINRKETDKIDLNFCEKIDTISYCDEIYKLVSPVNLKG
                     KVSKTNKGLYLDIDVNFTIVDNCSRCLKEVEIPLEYSIQGFLVKEEDYDEDEFEEFDP
                     FIFDGEEIDLIDIIEQTLDFNVPHKVLCSENCKGLCQVCGANLNEEECSCSEITNDEE
                     YIDPRFAKLKDLFN"
     gene            2024..2200
                     /gene="rpmF"
                     /locus_tag="ACGI59_000415"
     CDS             2024..2200
                     /gene="rpmF"
                     /locus_tag="ACGI59_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFG9738228.1"
                     /translation="MAVPKRKTSKSNTKMRRAANSKMEATGFVSCPQCHEPKLPHRVC
                     PDCGYYKGKEVVSK"
     gene            2407..2964
                     /gene="fapR"
                     /locus_tag="ACGI59_000416"
     CDS             2407..2964
                     /gene="fapR"
                     /locus_tag="ACGI59_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045717 - negative regulation of fatty acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor FapR"
                     /protein_id="HFG9738229.1"
                     /translation="MKKKSKAQRQKELIDMLKTDPFYTDEELSSLFDVSIQTIRLDRM
                     SLNIPELRERVKSIAETQSSKVKTLGVKEITGEIIDLSVGRLGISMLEVTQDMIYSKT
                     NTLKDTYIFSLADSLAMAIIDAPKVIMRVANVKSFKLIEQQDRLIAKAEVYRNIDKKH
                     YVKVVINNKAQEQIFRGKFIFEELD"
     gene            2975..3997
                     /gene="plsX"
                     /locus_tag="ACGI59_000417"
     CDS             2975..3997
                     /gene="plsX"
                     /locus_tag="ACGI59_000417"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFG9738230.1"
                     /translation="MKIVIDGMGGDNAPKSNVEGAVNAIKEYQVDLIITGDKDLLEKE
                     FSNYEFDRNKLEIVHTTEIIENEDKPVKAIRSKKDSSMVVALNLVKEGKADAIISAGN
                     TGALLAGGLFVVGRIKGIDRPCLCSAIPNVKRGMTLIADCGANADCKPKNLVEFAAMS
                     NIYSRKVLGLENPKVALANVGLEEGKGNDLVKRSYEEIKKLDLNFIGNVEAREVINAY
                     TDIIICDGFTGNILLKSAEGVALSVMSLIKETFMASTKSKIGALLIKDDLRKLKSFID
                     YSEYGGAPLLGLNGGVIKAHGSSDAKAIKNAINQGIKFSKGKVVEDINQFISKYNEEN
                     KNNEDE"
     gene            4023..5009
                     /locus_tag="ACGI59_000418"
     CDS             4023..5009
                     /locus_tag="ACGI59_000418"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFG9738231.1"
                     /translation="MNTKAGILGVGSYLPEQSYDNFHFEKIMDTSDEWISTRTGIKER
                     RFAKESEATSDLASKAALKAIECAKLNVEDIELIILATITPDMSLPSTACIVQDAIGA
                     VNATAFDISAACSGFVYGVTIAKQFVETGCYKNVLVIGAETCSKFLNYDDRTTAVLFG
                     DGAGAAVIGPVNEGGILSTHMGSDGKGKDCLKVPAGGSRLKASKETVEANLHTIEMAG
                     SDVFKFAVRKMAETSLRALEKANLNTTDIDYLVPHQANIRIIQASSKRLELDMKKVYV
                     NIDKYGNMSAASIPVALDEAYREGKIKKGDNVVLVGFGGGLTWGASVVKWTL"
     gene            5118..6047
                     /gene="fabK"
                     /locus_tag="ACGI59_000419"
     CDS             5118..6047
                     /gene="fabK"
                     /locus_tag="ACGI59_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HFG9738232.1"
                     /translation="MNKICKILNIKYPVIQGGMAWVATASLASAVSNAGGLGIIAAGN
                     APKEAIKKEIVECKKLTDKPFGVNVMLMSPFVDDIIDLIIEEKVQVITTGAGNPAKYM
                     DRLKEAGTKVIPVVPTIALAQRMEKLGATAVIAEGTEGGGHIGELTTMVLVPQVADAV
                     NIPVIAAGGIVDGRGIAASFALGASAVQVGTRFICSEECSVHSNYKNLVLKAKDRDAI
                     VTGRSTGHPVRTLKNKLSKEFLKMEQNGATPEELDKKGTGALRFATVDGDIEKGSFMA
                     GQSAAMVKEITPCKEIIEAMVNQAREIMPAIEL"
     gene            6063..7013
                     /gene="fabD"
                     /locus_tag="ACGI59_000420"
     CDS             6063..7013
                     /gene="fabD"
                     /locus_tag="ACGI59_000420"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFG9738233.1"
                     /translation="MGKVALVFPGQGAQYVGMAKDLYENNAVAKSVIDEASDALNMDL
                     KNLMFNGNEEELSKTENTQPAMVTHSVAVLKAVQAQIDLKYNACLGLSLGEYSALVAA
                     DAIDFKDAVCLVKKRGKFMQETVPAGVGAMAAVLGLDRNVLENVVKDIQGGIVEVANY
                     NSPGQIVISGENEKIEEAMVKCKEAGAKRAVKLNVSGPFHSSMLKEAGVKLASELEKV
                     NITKPKVDVVANVNADYYKNEGKDLLIKQVSSSVLWEDSIERLLNDGYDTFIEMGPGK
                     TLKAFIKKIASNKKANVNIYNIDGIDSLNEFVQNYRNGEI"
     gene            7013..7762
                     /gene="fabG"
                     /locus_tag="ACGI59_000421"
     CDS             7013..7762
                     /gene="fabG"
                     /locus_tag="ACGI59_000421"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HFG9738234.1"
                     /translation="MINLTGQVAVVTGGSRGIGKEIAKKLASFGADVVINYTSKEDEA
                     LKTKNEIESMGVKCTSIKCDVSKFDEVNQMIDSVVSEFGKIDILVNNAGITKDGLLMR
                     MKEEDFDRVIDINLKGVFNCTKAVTKPMMKKKYGRIINMTSVVGIMGNAGQTNYCASK
                     AGVIGFTKASARELASRNININAVAPGFIETDMTKVLSDDVKESTLANIPKKSYGKPE
                     DVANAVAFLVSDMSSYITGQVINVDGGMVMQ"
     gene            7816..8040
                     /gene="acpP"
                     /locus_tag="ACGI59_000422"
     CDS             7816..8040
                     /gene="acpP"
                     /locus_tag="ACGI59_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFG9738235.1"
                     /translation="MFNKVVEIIIEQLGVEDKEITMETSLMKDLEADSLDAVEIIMAL
                     EDEFGIEIPDTEAENFKSIGDIVKYIEANK"
     gene            8069..9307
                     /gene="fabF"
                     /locus_tag="ACGI59_000423"
     CDS             8069..9307
                     /gene="fabF"
                     /locus_tag="ACGI59_000423"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFG9738236.1"
                     /translation="MNKRVVITGLGCVTPLGTGKEEFWSNIKSGVSGIDKITNFDAST
                     YQTQIAGEVKNFHPEEYISKKELKRLDKFAQFAIVSAKLAVEDANLDLDKVDRERFGV
                     IIGSGIGGVEAIETQHKILLEKGNKRVSSLFVPMMIGNMAAGQVSIFLGAKGPNTNVC
                     TACASGTHSIGDAFKVIQRGDADLMVAGGSEAAVTGLAFAGFCNMKAMSTRNDDPKTA
                     SRPFDKDRDGFVMGEGAGIVILEDLEHALARGAKIYAEVVGYGLTADAYHMTTPAENG
                     EGAARSMNMALKDGNVPLEEVDYINAHGTSTYYNDLYETMAIKTVFGEKAYDLCVSST
                     KSMTGHLLGASGAIEAVVCAMSIEDSFVPPTINIQEVGEDLDLDYVPNQGKEKNIRYA
                     LSNSLGFGGHNATIVLKKYV"
     gene            9533..11251
                     /locus_tag="ACGI59_000424"
     CDS             9533..11251
                     /locus_tag="ACGI59_000424"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFG9738237.1"
                     /translation="MNFRLPKKRLPKKIIFICIILISIILFIKYDQNGLKEREIINKG
                     KYMSESLVADKEYDEAKKVVKTSFESIPEKKYNKVYNEVWDMLKTISYVPDGTKIVID
                     SLEKMRESDVKLSDECRFNIEKRLASVYIMDNNYSKAVDLTVKSIKLAEKLGNNYEKA
                     RLDVDLASIFLKIGGYETGEKLIKDSFKIDIDEGEKNGMVRLYAIINLAEIYVEEGEY
                     DKAIEISSRIKDYKKFVNTDNYNDFDIMASIMQSNAYVGKNNIQKAKTFLEKADYLIK
                     SDRTVYILDKDMYYYMAMGNLEYKSENYNSAIDNYKKSLEISTKRKLTQEKIKNLNKI
                     LNIYEKQGNIESLNEYQKMLINEYKENKSIRDSETSFYIIDKVSNESKLFEKTKKEIK
                     YYKILFTVILIAILGSTFLYNRLKYFKAQNLHDGLTNVYNRKSFDIMYEKYREKDDNF
                     ALVMIDIDNFKLINDNYGHKFGDTVIKGITNTICVMLEQDDKVFRYGGEEFSVLIRNK
                     SGDEVADIIDSIRVAIANKKWNDDVTVTISAGVAHVSDSKDVLEEADKNLYKAKQLGR
                     NKVVYK"
     gene            11282..13282
                     /locus_tag="ACGI59_000425"
     CDS             11282..13282
                     /locus_tag="ACGI59_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFG9738238.1"
                     /translation="MKQSIAIVTDKITKLSSFLEENIRLVLGNYIDINHYYLEVLKEN
                     DKIQGDFVLVMNDDRLNSMRKHLVQDSKIIVIRRTLKENEIYGLFSIPNGTEVLVVND
                     TKETTLETISLFYKIGVKNLKFTPYIEGKIYKNIDIAITPGVKGKVPSYINKVIDLGN
                     RYIDISTFIEIINGLRIDVKEIRKNLMAYSESLISLDNGVKDNYRQLFLKIEEQDVIL
                     NLSKDGIIFTSADGIINTFNKEALKILGINENPQGKNIEDIISEDLNILLEDKEIIDE
                     VVFTNKKYINVNKKSIFSMGNKAGIYYNLQEITYIKKLEQNLTNKLREQGQIARYTFK
                     DIKTKNKNMVKCVELAKKISKSDLSVLIIGESGTGKELLSQSIHNDSNRSNQPFIAVN
                     CAAVPDSLLESQLFGYEKGSFTGALREGKKGLFELANNGTIFLDEIGDMPPLLQTKLL
                     RVLQEKQVMPIGSHNVINIDVRVIAATNKNLLKMIREGKFREDLYYRLNVLPIDVPSL
                     RNRKEDIITLMNFFLKNNIRLSPEVITILEKYNWPGNIRELQNVASYVSLMCDSMVFR
                     DDLPPYIRVECANNYEDGFGYTRKEFEEILRILYERRESSSGIGRGFIIEKLIQEGIE
                     ISESKVRKVLSYLSECGYISSKSGRGGSQITSEGIKLYNNIN"
     gene            13633..13878
                     /locus_tag="ACGI59_000426"
     CDS             13633..13878
                     /locus_tag="ACGI59_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03905 family TSCPD domain-containing
                     protein"
                     /protein_id="HFG9738239.1"
                     /translation="MKYKTTGTCATEIEFEVKENKVTNVNFIGGCDGNLKGLKVLVEG
                     MNIEDVIQKLKGIECKTKPTSCPDQLSLALENYMNIK"
     gene            13913..14638
                     /locus_tag="ACGI59_000427"
     CDS             13913..14638
                     /locus_tag="ACGI59_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HFG9738240.1"
                     /translation="MKPIIGILGNLIIMENGMFPGLERSYVNNDYINAVLKGGGSPVI
                     IPVNTDKEVIKKQIEMVDGVLISGGWDINPQLYGEETREETTFIYPEVDEFDLIAISI
                     ALELKKPILGVCRGLQILNVSLGGTLYQDNNLIEGSYIKHTQSSKRHVATHKVDVKEG
                     SILEGILGKQLLTNSYHHQSVNQLGKGLKAIAYSKDGIIEAIEKEDENFVVGVQWHPE
                     MMVDYCDKMEKLFKYFINICSNL"
     gene            14660..15973
                     /locus_tag="ACGI59_000428"
     CDS             14660..15973
                     /locus_tag="ACGI59_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFG9738241.1"
                     /translation="MNEKNKMGLISIILLGINAVVGAGVFLLPGDAMKSFGVASIFVY
                     IFDMLLVLSMAFCFAEVAGKFNKNGAAYVYTKEAFGDFCGFEVGLMKWVIGCISWGAL
                     IVGFPTSLSAVWAPAGEPHIQKIIIVAMIVGLTIINLLGVSLSKIVQNVITVGKLIPL
                     ILFIGIGIFFIKGVNFTSSTMVPPGAGATEFGAAALLMFYSFTGFESIAVAAEDMENP
                     QKNIPIAIISVIVIASIIYILNQVVCVGILGDSLSSTSTPVADAARICFGNMGAGLVT
                     FGTLVSVGGICMCGAFVNPRSCVALADDKMLPRIFARKDKKGTPYVAIIATMLITIPI
                     ALSGSFAELAAISAVARFIQYIPTSLSVLVFRKKRPELVGTFKTPFGAVIPLIAVCVG
                     TWLLFQASMHQLIMGLGALAIGVPLYFIMKSYNRKIYGENLKKIV"
     gene            16179..16718
                     /locus_tag="ACGI59_000429"
     CDS             16179..16718
                     /locus_tag="ACGI59_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HFG9738242.1"
                     /translation="MPTITLKNGVDVLIREGVREDAQSIIDFYNEVGGETHFLSFGKD
                     EYKISLEEQENAIESAKASDNSVKLIAFIDGEIVGIATIDSNQKAKGKHVGVLGIVVK
                     EKYWGIGLGKRLMLDLIEWCKSNGITKKITLVTNEENYNAIGLYKKVGFEVESILKKE
                     CYYNGVYTDLIGMSLLLGI"
     gene            16807..18792
                     /locus_tag="ACGI59_000430"
     CDS             16807..18792
                     /locus_tag="ACGI59_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1,6-bisphosphatase"
                     /protein_id="HFG9738243.1"
                     /translation="MKNLCKISDDYLNSKLKYLKLLSKQYPSISKASTEIINLEAILN
                     LPKGTEHFITDVHGEYEPFVHVLKNGSGVIKRKIEELFSNTIRDSEKKMLATLVYYPE
                     QKLDLIIKQEENIDDFYRINIYRLIELCKYASSKYTRSKVRKLLPENFKYIIEELLHE
                     HVKSEHKEEYYKSIVETIVDIGIAKEFIIAISTVIQKLVVDRLHVIGDIYDRGPRPDI
                     IVDKLIEHHCVDIQWGNHDILWMGAASGEKTCIANALRISARYANLDIVEDIYGINLL
                     PLATFAIEMYKDDPCKEFIPKVNDQSVTTTEKSLMAKMHKAISIIQFKLEGEVIRRRP
                     EFEMEHRLLLNMINYDEGTINLKGKTYKLKDTYLPTIDKKDPYKLTMEERNVIDKLVS
                     SFRGSEKLQKHVSFLFSKGSIYLKANSNLLIHGCVPLNEDGSFMSMNIMGKEYKGKAL
                     MDKMESLAREGFFFKDKAEEKLYGMDIMWYLWTGKCSSLFGKDDMTTFERYFIAEKET
                     HKENKNPYFKLRENEMACKRLFEEFDLELDESHIINGHVPVESKNGESPIKANGKILV
                     IDGGFSRAYQKTTGIAGYTLIYNSRTLQLVSHEPFNSAEEAIANESDILSTTVVVEHK
                     AKRKMVRDTDEGIKIQEEIEDLKLLLMAYKKGLIKEM"
     gene            19016..19984
                     /gene="spoIIIAA"
                     /locus_tag="ACGI59_000431"
     CDS             19016..19984
                     /gene="spoIIIAA"
                     /locus_tag="ACGI59_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AA"
                     /protein_id="HFG9738244.1"
                     /translation="MNKLSDEIINSLSTTIREKVEKVSNNNLNIEEIRLRSQKPLILN
                     ANSKDYFYNQKTMTLDLNQQNSYVVTREDVEQTFQIICKYSIHSFMDDIKKGFITLRG
                     GHRVGLVGKAIVEDGQVKNIKHISSLNIRVSREIIGCSDKILSHIIKGKNQINNTLII
                     SPPQCGKTTLIRDIVRNLSNGNEDYGFKGLKVALVDERNEIAGAYLGVPQMDVGIRTD
                     IIETCPKDLGITMLLRSMSPNVIVTDEIGSEKEIKALYTALNGGIGLITTVHGDSIED
                     IQNRKELNRLLDKELFKKVIILSAKRGAGTIEKIYDLEEKRWYFAN"
     gene            19974..20495
                     /locus_tag="ACGI59_000432"
     CDS             19974..20495
                     /locus_tag="ACGI59_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AB"
                     /protein_id="HFG9738245.1"
                     /translation="MQIKIIIIAFLIGSSYLIGEQIYKTYTRRHKQLNDLIRVLEILR
                     MDLSFGLYTLEEIFNRIGGNKEFCFWKFFYQISEGLHNEQSKTLEIIISENIDVLSKE
                     TYLGNKEIEELKNLILTLGKSDIESQQRMIDLSIENLKKQTYETKEDINKKGVLYKKL
                     VTFIGIGICIILI"
     gene            20512..20709
                     /gene="spoIIIAC"
                     /locus_tag="ACGI59_000433"
     CDS             20512..20709
                     /gene="spoIIIAC"
                     /locus_tag="ACGI59_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018589775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AC"
                     /protein_id="HFG9738246.1"
                     /translation="MEISLILKVAGVGILISVLNMILEKTDRKDWAGLTTLAGVIIVL
                     GMVITEISDLFNTVRTMFQLY"
     gene            20734..21111
                     /gene="spoIIIAD"
                     /locus_tag="ACGI59_000434"
     CDS             20734..21111
                     /gene="spoIIIAD"
                     /locus_tag="ACGI59_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AD"
                     /protein_id="HFG9738247.1"
                     /translation="MQLIGIAIISTTLCLVIKKDRPEIANFIAIITGVIILLSVMFKL
                     NFIVDSIQDLANKANIPTMYISLIIKLIGIAYLMEFAIQLCKDCGEGNIASKLEFGGK
                     IIVMSMSFPILLSIVEMVVNIIP"
     gene            21196..22401
                     /gene="spoIIIAE"
                     /locus_tag="ACGI59_000435"
     CDS             21196..22401
                     /gene="spoIIIAE"
                     /locus_tag="ACGI59_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AE"
                     /protein_id="HFG9738248.1"
                     /translation="MKKRFLPMIIGFLFTFFFVNIFAIMIFANEVPSSEDKEYGETKN
                     SIDKYIDGQLDKLDINEIQDYINKEIVINDVNLKSFVKDLISGEKNILDLFNKDGLKI
                     LMFDEFKASLKVVAVILVLALLSSILKSLENSFSSGAVSQIATYIIFITMVSLTLIGF
                     KDVLQICYDAIDHTVGLMQVIMPILITFLLLIGFPITSTTLNPIFIGGVTFINVFFKN
                     FLFVSITVAFGILIINNLSKNIRLKRFFSFVKQINYVSIGAMFTVYLGLVSIQGLYVT
                     SFDKFSVKTAKFAIGNFIPVVGGFVSDSVDILLSSSQLIKNIFGGIGLILLVGICLLP
                     VIKILSVIVVYKLAAIIVEPVGEDGISNFLNEVANLMIIMLASVIAITVMFFVTVAIL
                     TSISVVSQG"
     gene            22461..23078
                     /locus_tag="ACGI59_000436"
     CDS             22461..23078
                     /locus_tag="ACGI59_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AF"
                     /protein_id="HFG9738249.1"
                     /translation="MLEGIKAWIVSVLIGAFIVNIVDMILPSSKIKPYVNLVLNFMFV
                     FIVITPVVGFFSKDMSLEDRILKSMGNYNKQYVDSTNALAKETGNNSLSKGYEDGLKE
                     VLKLKLDEYGYDLEDIELNGANINNIKIKEKNNSAKSNNSSNEENKNNSTKREEENIN
                     SNDKENSKQVFKKGTEYGLNLNEEKLKNDLIKVLDVSIEDIQIDK"
     gene            23090..23644
                     /locus_tag="ACGI59_000437"
     CDS             23090..23644
                     /locus_tag="ACGI59_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AG"
                     /protein_id="HFG9738250.1"
                     /translation="MLKNLNDKDKRKVYSLITIAGICVVSLVLLSCFPSSKSDKQVGK
                     TETNKNAEKQVTKEQEKDDLESKLTAILSKIDGAGDVDVMVTFESSEEIQPAFNSNNT
                     TETTEEKDAQGGERTVTTSSENKTMITSNSSDPVVIKTTEPKIKGVIVVASGASDPSV
                     KETLYSAVQTSLQVAGHQVEIYSK"
     gene            23671..24360
                     /locus_tag="ACGI59_000438"
     CDS             23671..24360
                     /locus_tag="ACGI59_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIIAH-like family protein"
                     /protein_id="HFG9738251.1"
                     /translation="MKFNYKGRGFVIITLTAMLVVVGTVNYQLSKKSLLETSKEFKAY
                     EEAQLQKNTDESDSSKTEDSSNVDKQDGKESADIDIVDSKASKVKEKATETSKEIKAQ
                     LSSEKNMKKASYILDMKMNREKQRNELVQDLNEMINNPSTTEESRKEASNMKLNIVKI
                     QEKELQIENLLSTKGYEEALVYISDNKVNVVVNEAKLEKKDAAKIFDLVAEQANVKYE
                     NIKLTNNNSNK"
     gene            24470..24832
                     /locus_tag="ACGI59_000439"
     CDS             24470..24832
                     /locus_tag="ACGI59_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFG9738252.1"
                     /translation="MEDNKFGQVKISNDVIATIAGLAALEVEGIETTATLTDKLLKNN
                     GVKIQIEEEDVNLDVMVTIKYGMSIPDTAFKVQENVKNTVETMTGLKVSQVNIHIQGI
                     SFKKDKVDKEEAKATKKN"
     gene            24917..25423
                     /gene="nusB"
                     /locus_tag="ACGI59_000440"
     CDS             24917..25423
                     /gene="nusB"
                     /locus_tag="ACGI59_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFG9738253.1"
                     /translation="MKKDRAQKSTTREYIMKLIYQININKEDFETLEDKVDNFLKDNS
                     EHIINRYKELALQYSKNTNLKLEDTEIEDVIDKKYINTVCKALKENHDKIDELINKHA
                     KNWTVDRMPKVDVSILRLSVCEILYLDTPNKVSINEAVELAKIYCDDKSPKFINGILG
                     SVVDEIGK"
     gene            25407..26477
                     /locus_tag="ACGI59_000441"
     CDS             25407..26477
                     /locus_tag="ACGI59_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-sialoglycoprotein endopeptidase"
                     /protein_id="HFG9738254.1"
                     /translation="MKLGNNIIIGIDTSCYTTSIAAISLDKKVIFNEKIMLEVRDNSK
                     GLRQSEAVFQHINNLGILSDRIKSFKDKFNVEGVCSSKKPRPVENSYMPVFNVGHNFG
                     KLLSSIYGCRFYETTHQENHIEASLLNSKLKNNNKFISVHMSGGTTEILLTSKQDSHH
                     NVCDTNLGKIAKISIKKDDKSKLYNNFGYNIDIIGGSKDISFGQLIDRVGIKLGYKFP
                     SGKYLDENALNCNLKIESGLKTSVRDGYMNLSGLENQVNKIINDNGDNTNQKEYISKL
                     VLDSVVRNMFKSLVYLCETYNVNEVVFAGGVSASKYILRELSMKLRKKHIEAYFTEPQ
                     YSTDNAVGCAIIGLNNFLGERV"
     gene            26474..27679
                     /gene="xseA"
                     /locus_tag="ACGI59_000442"
     CDS             26474..27679
                     /gene="xseA"
                     /locus_tag="ACGI59_000442"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFG9738255.1"
                     /translation="MKLRALDISEANSYIKRILINDPILSNLKVKGEISNFKVHSSGN
                     VYLSLKDETSKLNCVIFKSNFNRNLKLDNGVKIIANGYISVYERDGAYQLYINEIEIE
                     GIGNLHIEFNRLKEKLNKEGLFDPKYKIPIPKMPNSIGVITSPTGAVIRDIINVIKRR
                     YPKVNIKLYPVTVQGDKSAEEICEAIRFFNHMKNVDTLIVGRGGGSIEELWSFNDEMV
                     AREVFNSQIPIISAVGHETDFTICDFVSDMRAPTPSAAAEIATPSLDDINYKLGNIKS
                     RMSKSLTNQIELDQYRLETVFNKINNYLDSYTIKDKVIQLDKIYDKIIFGIENNLKLE
                     DEKLVKIGALLHNLSPLATMDRGYSITQKNGKVINSIKGLKIKDSIDIVLKDGNLECM
                     IDKIENKEG"
     gene            27681..27896
                     /gene="xseB"
                     /locus_tag="ACGI59_000443"
     CDS             27681..27896
                     /gene="xseB"
                     /locus_tag="ACGI59_000443"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFG9738256.1"
                     /translation="MNLTYEEAYKRLESILSELESKNASLDESLSLYEEGISLYKHCN
                     KLLDDAKLKISKFNQLGIEEDFKIEEE"
     gene            27899..28786
                     /locus_tag="ACGI59_000444"
     CDS             27899..28786
                     /locus_tag="ACGI59_000444"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFG9738257.1"
                     /translation="MEFKQCLKEKASFVEKVLKEYMPKEEGYQKTVIEAMNYSLSAGG
                     KRLRPILTLEACKIVGGNEDEAIPFAIAIEMIHTYSLIHDDLPALDNDDLRRGRPTNH
                     KVYGEAMGILAGDALLNYAFEVMLAGSINKENPEKYLKAINEIAKGAGIYGMIGGQVV
                     DVESENKQIGKEKLDYIHMNKTAAMMVGCMRAGATIGGANSEQMEEITKYAKNIGLSF
                     QIVDDILDIVGDEAKLGKKVGSDIENHKSTYPSLLGLDKSKEIAHNLIDEAKKSIEKL
                     SDDVDFLKGLAEYIIDREY"
     gene            28816..29253
                     /locus_tag="ACGI59_000445"
     CDS             28816..29253
                     /locus_tag="ACGI59_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent PAP2 family protein"
                     /protein_id="HFG9738258.1"
                     /translation="MDFFSEIFNNGALGISLIACFLAQFIKIFTGKEKRIELSRILIS
                     GGMPSSHSSFVTSLATVVGIEKGFNSTDFAIITVLALIIMYDAAGVRRAVGKQATILN
                     QMVADIQHGKHIEQKKLKELIGHTPLEVWFGALLGIVTALILM"
     gene            29287..31152
                     /gene="dxs"
                     /locus_tag="ACGI59_000446"
     CDS             29287..31152
                     /gene="dxs"
                     /locus_tag="ACGI59_000446"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFG9738259.1"
                     /translation="MYKYLDKVNSPKDIKNMSIEEMDLLAKDIRKFLVKSVSKTGGHL
                     ASNLGVVELTLALHKVFDSPKDKIVWDVGHQSYVHKIVTGRKDCFVSLRQFNGLSGFP
                     KENESPHDIFDTGHSSTSISIATGIACARDIKKENYSVISVIGDGSITGGMALEALNQ
                     LGYIDTNMIVILNDNEMSIDKNVGGMSKYLSSIIRNSTVEKMTDEVDKILNVTQTGEI
                     LSKTAHRFKDKLMYSFSPQDCSFFDSLGIRYYGPIDGHNTKELIDILRKAKHKKGPVL
                     LHVITKKGKGYRFAEEQPDKYHGVSKFDIKTGVTSAKVKSMSISVGEKLVDMANSNEN
                     IVAITAAMPSGTGLNLFESAYPKRYYDVGIAEQHATGFAAGLAKNGMKPYFAVYSSFL
                     QRAYDQVIHDVCITKKPVTFLIDRAGLVGNDGETHHGMFDLSYLNSIPNIVVMAPKDT
                     REMELMMDLSLKLDCPLAIRYPRGSSYYLDKGEYGEIVLGKYEVLDDGQDTVILCIGS
                     MVKHALEAKEILSREGINPTIVNARFLKPIDEGMLKALLKNHKNVVTIEDNIVTGGFG
                     SRINKFIIDNEYNVNILNIAIPEEFVKHGNIDELYDFVGLSPKSIADKIRKLVIE"
     gene            31285..32097
                     /locus_tag="ACGI59_000447"
     CDS             31285..32097
                     /locus_tag="ACGI59_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family rRNA
                     (cytidine-2'-O)-methyltransferase"
                     /protein_id="HFG9738260.1"
                     /translation="MKKRIDLLLVEQGYFESRERAKKAIMAGLVFVDNQRCDKAGTEV
                     KEDCSIEVKGNPIPYVSRGGLKLEKAMKNFDLTIDGKVCMDIGASTGGFTDCMLKNGA
                     IKVFSIDVGYGQLAWKLRQDDRVVCMERTNIRNVTIEDTKQFADFASIDVSFISLKLV
                     LPKAKELVRHDGEVVALIKPQFEAGREKVGKKGVVREKSTHIEVIKMISDFSVENGFE
                     ILGLDFSPIKGPEGNIEYLIHLRNGNEGYEFDGETYNNKIVEVVEASHNLDK"
     gene            32117..33796
                     /gene="recN"
                     /locus_tag="ACGI59_000448"
     CDS             32117..33796
                     /gene="recN"
                     /locus_tag="ACGI59_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFG9738261.1"
                     /translation="MILELYMKNCALVEELRLNIDKNLNILTGETGSGKSIIIDALGL
                     CLGDKYDRSFLRKGTDKGLVEAVFFSDNRYLKKILEENDISMEDDNLLVITRLIYSDG
                     KSTARVNGRTVKVSLLKEIASTLIDIHGQHQNQALFNKDTHLKFLDLFGENELEEFKI
                     AYKNVYHKYSEVKKALNCLTENKDEMQIQREIDLLRFQINEIEAANLNKNEYEDLLKQ
                     REVYRNSEKIYNNLNSSYSKLHNGEYNVIDLIGLASKELNDISKYDSVLSEYSDTVER
                     IMYELQDISGEIRNYKDNIDFEPYELEQIELRIDEINNLRRKYGDSIDDIFEYYGKIK
                     DRLDEILNRDERVEQLRSKLMNIEEDLKIKASKLTKARIEVATTLEKILLDELKSLNM
                     KNVMFKVNFEKGPFTLDGVDDIEFMISFNLGEDIKPIYKVASGGEMSRFMLAFKTILA
                     DIDDIDTLVFDEIDTGISGIAAQIVGEKLSDIAKKKQIICITHLPQIAANADTHYCIE
                     KDTSNNRTFTNVSKLNESQRKNEIARLIAGNNITEKTIEHASEIIELAKKC"
     gene            34010..34876
                     /locus_tag="ACGI59_000449"
     CDS             34010..34876
                     /locus_tag="ACGI59_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9738262.1"
                     /translation="MNKKDTMHLIFTIVIFILLWYIVVAVSEINKNIESCSNKLDYLS
                     QEIMSTKSDISNTINEEMSKSYITKSIDLKVKKIEKKECVIDVDVQLSKLGKNGKVFF
                     MYKEDSDKWNETEMTKHGELSYACEIKINTGSEYDYKVVTSGAISESSDVQKLTKSDY
                     MPEQPIFNSGIRDNREYFIEIVENSNLFNHESEKSKNKVYDNIRLEKVDIIVNEGKKD
                     TIYKAKLAEGLDDGKTNNSNRNQVNYEVSFPKRDIDDIIYIKAKLTYNNGVEYTKDIT
                     EDITMGLQDLEK"
     gene            complement(34969..35448)
                     /locus_tag="ACGI59_000450"
     CDS             complement(34969..35448)
                     /locus_tag="ACGI59_000450"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFG9738263.1"
                     /translation="MAQNITLQFVEEKDLENLENVKLLFTEYSNSLNIDLCFQDFNNE
                     LKTLPGKYKKPSGSLILAFVDENLAGCVALKKLEGKICELKRLYVRNQFRGLKIGKIL
                     LEEIIEEAKKFGYTHMRLDTLPSMKSAQGLYEKFGFYDIEPYTYNPIEGARYMELKL"
     gene            complement(35521..35961)
                     /locus_tag="ACGI59_000451"
     CDS             complement(35521..35961)
                     /locus_tag="ACGI59_000451"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFG9738264.1"
                     /translation="MKLNVRIADINNWHDLVSLSVDKSQLDYIESNALSIAESKFITA
                     WVPVGIYDENSLIGFAMYGRLEDDRVWLDRFMIDSKYQGKGYGKASLDFLVNHLKNEY
                     NCDELYLSIFEDNKMAIKLYKDFGFEFNGELDYGGEKVMVLKSN"
     gene            36122..37180
                     /locus_tag="ACGI59_000452"
     CDS             36122..37180
                     /locus_tag="ACGI59_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIVB peptidase S55 domain-containing protein"
                     /protein_id="HFG9738265.1"
                     /translation="MNKINKNFKNIWIILGAVSIFTMVIIFNKNCNVNKETQIEAFTI
                     KNHDKKFVYPMGNIIGVKANTDGVLVLGYEEEDVDYIGGIQIGDNIVKIDNKRIKNSQ
                     DVSEILNKIKKSKVEVTFERKNKYKTEVIETKKENGKYKLGLWVRDKISGIGTMTFYD
                     PSMEKFKAIGHAIKDSDTNELLKIKQGYIYKPEQLKIVKASNEKVGKIKGDFNDSNLM
                     GNFSNNSELGISGNITENHNKEFNVANKEKQLIEVGRPQDVKIGDAVILFEDKNKNIT
                     SYDIKIESIVYDKGNYRDMVIKVVDDKLLEYTGGIVQGMSGAPIIQNNKIIGAITHVF
                     RDNPKKGYGIFIDEMIKL"
     gene            37322..38146
                     /gene="spo0A"
                     /locus_tag="ACGI59_000453"
     CDS             37322..38146
                     /gene="spo0A"
                     /locus_tag="ACGI59_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0042173 - regulation of sporulation
                     resulting in formation of a cellular spore [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA];
                     GO_process: GO:0051606 - detection of stimulus [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcription factor Spo0A"
                     /protein_id="HFG9738266.1"
                     /translation="MGGFLVEKIKIVLADDNKDFCQVLKEYLSNEDDIDILGIAKDGI
                     EALDLVKKTQPDLLILDVIMPHLDGLGVIEKLNTMDIPKMPKIIVLSAVGQDKITQSA
                     INLGADYYIVKPFDFVVFINRIRELVSNRVTQVEPKPRPVQETQMTRSDFVKNVGNIE
                     TEITNIIHEIGVPAHIKGYLYLREAIKMVIDNVELLGAVTKELYPSIAKKFNTTPSRV
                     ERAIRHAIEVAWSRGKVDTINQLFGYTVHNTKGKPTNSEFIAMIADKLRLEHSMVK"
     gene            38559..39680
                     /gene="steA"
                     /locus_tag="ACGI59_000454"
     CDS             38559..39680
                     /gene="steA"
                     /locus_tag="ACGI59_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cytokinetic ring protein SteA"
                     /protein_id="HFG9738267.1"
                     /translation="MRVEAPIKVDRKTKKLAKRVESGEIAVINHIDIDEVAANSLVEA
                     KIKLVINAAPSISGRYPNKGPGILTENNILIIDNVGEELFEELKEGETIEVVDGKIYR
                     KGKFLGAGEVLDKYEVSHQIKAAYENLAVELDRFIDNTIDYAKKEKGFILGEVEIPKV
                     KTNYANKHVLIVVRGQDYKEDLSTMLSYIEEVKPVLVGVDGGADALIEFGYTPDVIVG
                     DMDSVSDEALKKASEIVVHAYTDGRAPGLKRVEELGLDAVVFPAPGTSEDIAMLIAYE
                     YKAELIVAVGTHSNMIDFLEKGRKGMASTFLVRLKIGSKLIDAKGVNLLYRSKLKIKY
                     IWALIATALFPVLVVASLSPGVQQFIQLMQLKFRVLLQM"
     gene            39698..40573
                     /locus_tag="ACGI59_000455"
     CDS             39698..40573
                     /locus_tag="ACGI59_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper transporter"
                     /protein_id="HFG9738268.1"
                     /translation="MHINMKYYIVTIGAIFIALGIGMLVGFNLNNNQQLSEQQANIIN
                     DLDDKFNILKEKNDKLDSDLASVNRDYEEAVNFINKNVDKILAGSLNGKSIGIISANE
                     NDDYTKNIEDIINKSNGSIAFNIILKENITNPEKLKEMSTKLGTDVKNANDAVNYIID
                     TLKKEDASDILTYLQELDVIKFNFIGDTYLKYDSVVIAGGNDAKDSTKQFEKIEKFVV
                     SKLKSENKYLVEVQNTGVKTSYVELYSKNKVATIDNIDEGIGSISLAILLQQGNIVGN
                     FGRLDTATSLLPSIK"
     gene            40597..41289
                     /locus_tag="ACGI59_000456"
     CDS             40597..41289
                     /locus_tag="ACGI59_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFG9738269.1"
                     /translation="MDSYISIIIPAYNEESRIKYTLESILDIKQINEIIVVDDGSIDN
                     TAKVVSELENEKIKFFKLDKNRGKGYALNYGIKLAMKNADIIGFLDGDLGSSAKEVEK
                     LIVPILNGEADVTIAKFPPAKRKGGLGFVKGLAKNSVFEMTGVELDATLSGQRIFKRE
                     VLEKFDEIPFGYGVEVGMTIDILKYGFKIKEVLVNMTHNETGRNLKGFIHRGKQYYHI
                     KKVLGQKKKEWR"
     gene            41297..42121
                     /locus_tag="ACGI59_000457"
     CDS             41297..42121
                     /locus_tag="ACGI59_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HFG9738270.1"
                     /translation="MKDYILYTILLLTGLFGTYAVIPLFKNLLINGNVLRPNYKKDMI
                     PVSMGIVFLPMIVINGIILAFFTTEFKNLAYIFMFMFGMISMFFAGILDDVIGNRDVS
                     GLKGHFKSLFKGSLTTGGFKALFGGFVGLIVSVAISKDIIDIIVNTLIIALSTNLMNL
                     LDLRPGRAIKAYLFIMVIVFLTLTGFVQVLPLLIVPNVLAYFNYDLKARAMMGDTGSN
                     VLGISIGMLISFGYPFNIRLGWLIFVVIMHIFTEKYSLTKIIEKNKLLNFIDKLGR"
     gene            42126..43193
                     /locus_tag="ACGI59_000458"
     CDS             42126..43193
                     /locus_tag="ACGI59_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3866 family protein"
                     /protein_id="HFG9738271.1"
                     /translation="MISKRVGIVESIVSQTETLDDIRVNINGEIQKAYNYPKISGTIN
                     IGDEVVLNTTAVELSLGTGGYHFVITNLNNIESTLTEGGHIMKLRYTPLQIKVDSVEE
                     QESIYHDKFANFKGLEGLSVVVGTLHSMLTPFAASFKRNNPNKKLVYIMTDGASLPIY
                     LSKNVDTLKEKKLIDSTITIGNAFGGDYECINIYTALITAKEILKADAVFVSMGPGIA
                     GTGTKYGFTGIEQGSILDAVKKLEGRAIAIPRISFADKRERHQGISHHSMTVFKEIVN
                     VNVDIPITIYDDEKLNFIKEQINLNGLDKKHNIIYIDNNKTKEDLDYFNLKVKSMGRN
                     YEQDEAFFKAASTAAYYLMEV"
     gene            43195..43731
                     /locus_tag="ACGI59_000459"
     CDS             43195..43731
                     /locus_tag="ACGI59_000459"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFG9738272.1"
                     /translation="MVLEEKTISSDRVYTGKVITLKVDTVEIPGQGYQKRELVEVGGA
                     VGIVAITDDNKVVLVKQFRKPIEKPIFEIPAGKLEKNESPKECAERELKEETGYSAKN
                     IKLIHKFFTSAGFSNEIMFVYLATGLTPGENNLDADEFLDVYEIELEEAYNMVLKNDV
                     EDAKTSIGLLLVKDMFKN"
     gene            43799..44404
                     /locus_tag="ACGI59_000460"
     CDS             43799..44404
                     /locus_tag="ACGI59_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HFG9738273.1"
                     /translation="MRRTYRKNDFRELNRQIIIIGLLFMMSIVVGSYINKILPGSSNN
                     ILNNINPAVEYYNLNISIKDTVIQNLKSDAIFMGSIALLSLFVVTIPAVLVAFVLKGM
                     SIGYTINSCILALKFKSIKMILIILFKNLIIIPCAIILALISLSYFKEMVYEFKKKSR
                     KNMQFLIKRYILNIIIIIALSLGLQLILNTASIGIIKFLAK"
     gene            44416..45330
                     /locus_tag="ACGI59_000461"
     CDS             44416..45330
                     /locus_tag="ACGI59_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFG9738274.1"
                     /translation="MDIIEGYIDYIKDKKKLSENTVASYFMDIKKYMEYINQKSIKLV
                     DIVENDIISYLIKLEKDNVSIATIARMISSIKSFHDYLFLNHICTNNPAKDIKKPKIK
                     KENINILTEEEIERLLNFPKLTTPKLIRDKAIFEVLYGTGIKVSELVEMNIEDIDLDI
                     DYIYCNSCCKNQRVIPLCDITKLYLEKYLKEARPKMAAEGEKSLFVSSLGQRFTRQGL
                     WKVIKKYSNLANIDKNINPTMLRHSFAIHLLNEGANIAVVSKILGNVNLSSLQVYLNH
                     IDKNVRREIKEKHPRNDVDVELQKAEAK"
     gene            45372..46544
                     /locus_tag="ACGI59_000462"
     CDS             45372..46544
                     /locus_tag="ACGI59_000462"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFG9738275.1"
                     /translation="MSRVIWIVIDSVGIGALPDAEKFGDSKDVSTLGNIFKEYPDIQI
                     PNMRNLGIGNIEGIDFFESIKEPIGCFGKCKEMSQGKDTTTGHWEMTGIIVDKPFKTF
                     EHGFSKEIIEEFEKKTGRKVVGNKPASGTVIIDEYGEHQIKTGDVIVYTSADSVFQIA
                     ANEEVIPLEELYNMCKIAREIMMGDNAVARVIARPFIGKKKGEFVRTSNRRDYSLDPF
                     EPTVLDNIKESGLDVLAVGKIEDIFNGKGITDAIHTKSNMDGVDETLNYMKQDNKGLI
                     YSNLVDFDSKYGHRRDPEGYKKALEEFDSRLPEIMANMREDDILIINADHGNDPTYKG
                     TDHTREYIPVMIYGNKIKKGFNLGVKDTFADIGATVADILNVKLPKHGSSFKEDLF"
     gene            46591..47403
                     /locus_tag="ACGI59_000463"
     CDS             46591..47403
                     /locus_tag="ACGI59_000463"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFG9738276.1"
                     /translation="MFEKIAQSSKFINSKSNIKPKIGLILGSGLGDLANDIEDPVTIK
                     YSEIPNFPVSTVAGHAGQLVIGKLEGKEVIAMQGRFHYYEGYSQKEATFPVRVMKELG
                     VEILIVTNAAGGVNKEFKAGDLMLIRDHINFSGSNPLVGKNDDRFGARFPDMSDAYSS
                     KYVDVVKNCAKECNIDVKEGVYMFFSGPNYETPAEVRMAQILGADAVGMSTVPEVIVA
                     SHSNIGVIGISCITNMAAGILNQPLSHEEVIETTQKVKSEFMNLVKTTVKNL"
     gene            47607..48932
                     /locus_tag="ACGI59_000464"
     CDS             47607..48932
                     /locus_tag="ACGI59_000464"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HFG9738277.1"
                     /translation="MRMYDIIKKKRDNLELSKKEINYFIENYSKGDIPDYQASALLMS
                     IYLNKMNKQETVHLTEAMMNSGDMINLSEIDGIKVDKHSTGGVGDKTTIALIPLVASC
                     GAPVAKMSGRGLGHTGGTIDKLESIPGFSTEMEISKFIDSVNKSKIAVCGQSAKVAIA
                     DKKIYALRDVTATVDNISLISSSVMSKKLASGADAIVLDVKTGDGAFMKTLEESFELA
                     KSMVDIGTGMNRDTIGIVTDMDEPLGFAVGNSLEVIEAIETLKGNGPKDLVELCEVLG
                     AYMLVLSKVAHDFEDGVEKIRESIKSGTAIQKLKEFIENQGGNKDVVDDYSLFKKSKY
                     KYPVKSTKSGYISKIKAEDVGVSAMILGAGRETKDDILDLSAGIILEKKVGDYVNEGE
                     VLAYMYYNDEQKLSLAKGRFIDSYSIVDSKIEKNKLIYGIVTKEEIKKF"
     gene            49145..50224
                     /gene="mltG"
                     /locus_tag="ACGI59_000465"
     CDS             49145..50224
                     /gene="mltG"
                     /locus_tag="ACGI59_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HFG9738278.1"
                     /translation="MNFKENRLKIAVLIIVILIILAGIFVFIQIGPYDKNNKKDVIID
                     VPSGASVGKISDILYENKLIKNELLFKLLVKVSNKAPSIKSGTYLLNQSYSNNDIISL
                     LVSGKIYQDGIKVTIPEGATSKEIIAMLVSKNLGDKATFENLIKKPQEFYDKFSYLKE
                     DGITSLEGFLYPETYYFNSKKQSEEDILSEMLKVFDSKYTDKFKKKQKELNMTLQEVM
                     EMASIIEKEAVLDKDRPIIASVFYNRLKVGMPLQSDATIQYIFEERKKIVTYDDLKID
                     SPYNSYKNKGLPPTPISNPGIKSIEAALYPEKTDYLYFVAKIDGGNNYSTNYQDHLKY
                     VKEYKEARDKQSKDTKATNKENTKK"
     gene            50396..51070
                     /locus_tag="ACGI59_000466"
     CDS             50396..51070
                     /locus_tag="ACGI59_000466"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HFG9738279.1"
                     /translation="MSNIVNDLVEDYIRVTLKEKEGFLKDLEIYAEENSVPIIHKEVS
                     DLLKVLLKVQKPKRILEVGCAIGYSSIFFATIIGKDADIITVERNEKMIEKAKENIKL
                     AGFDNNITILEGDAEEKLSQVQGEFDLIFIDAAKGQYKLFFDLVIDKLKDGGLLVSDN
                     ILYKGMVAHDDFVVRRKKTIVKRMRKYLDYICNCDYLSTSLVPIGDGVALSYKESKRG
                     DVDGIK"
     gene            51057..52304
                     /locus_tag="ACGI59_000467"
     CDS             51057..52304
                     /locus_tag="ACGI59_000467"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1901576 - obsolete organic substance
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFG9738280.1"
                     /translation="MELNKVELLAPAGDLERLKIAITYGADAVYIGGEIFGMRSAAKN
                     FSKEDMAEGVAFAHERGKKVFVTVNIIPHNEDFLQLEDYLLELEEIGIDAVIIADPGV
                     LSVIKKVIPNMEIHLSTQANTTNYLSANFWYEHGIKRVVVARELSFDEIYEIRAKTPL
                     DMDIEAFMHGAMCISYSGRCLISNYMTGRDANKGSCAQSCRWQYHLVEEKRPGEYFPI
                     YEDERGTFFFNSKDLCMIEYIPELIKSGITSLKIEGRMKTAYYVATVVRAYRMAIDEF
                     YRDPENWKFNPMWMEELKKGSHRHFTSGFYLNKPTTEDQNYQSASYVRNYDFIGIVRE
                     TEDEDGLIVVEQRNKMCVGDEIEVMGPYKETMFTKIEEMYNEEGEAIESAPHPRQIVK
                     LKLSVKVGKDYMLRKVIEEKVEE"
     gene            52376..54046
                     /locus_tag="ACGI59_000468"
     CDS             52376..54046
                     /locus_tag="ACGI59_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HFG9738281.1"
                     /translation="MSKKKTPFLKKVGKRSWCIFTIILIIYSVLIYRLVDIQVLKGDK
                     YKQSVESQSVEKVELNSGRGIIYDRNNKKLTDTSKSQVLIVEKEKLNNNYKILELIKK
                     ATKMNDLDIYKAVQEQLTRPIIQIQTKNIDIDKSMKKELEKNGIMVEEKTMRYAKDGL
                     LSHTIGYIKEDDKSGQSGIEKSMDSVLRNSNEKYISAFKAGDAGNEKSLNILKGSVKT
                     VDNKDKDRHLKTTIDYNIQKKLEQILNKEENPTAAIISEASTGEILAMCSRPNFDQND
                     ISKSLKGKNGEFENRVIKATYPPGSVFKMVVLFSALENGVIDENYTYNCTGKTKVGNT
                     NEILRCNKRDGHGFQNLRQAFSNSCNPAFLDIAMKLGKEKILKSAEKLHLFEKVDIGL
                     DEEKIREAPKNISIRNLAIGQENIEFTPLQINQMTQIIANNGTFKPLYLYKSLVDNNM
                     NTIKTYKSSKKEELISPYVCTQVKEYMKSVSRTGTAKDLKDIEGGCGVKTGTAQSSLN
                     KKAIDHGWITGFYPEERPKYVITVLVEGTQKGNKSATPIFKEICESIK"
     gene            complement(54143..54427)
                     /locus_tag="ACGI59_000469"
     CDS             complement(54143..54427)
                     /locus_tag="ACGI59_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFG9738282.1"
                     /translation="MCNEISLLDILGTEANYVLDEVELKVQVGKLYEQLNKILTPRER
                     EIVQLRYGLTPYGYKTQREIAQKLDISRSYVSRIEKKALKKLEKELVQES"
     gene            complement(54453..55967)
                     /locus_tag="ACGI59_000470"
     CDS             complement(54453..55967)
                     /locus_tag="ACGI59_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFG9738283.1"
                     /translation="MSVAIYLRKSRADEEAEKQGEFETLSRHKSTLLKLAKEQNLDVI
                     EIKEELVSGESIIHRPKMLELLKEVEENKYDAVLVMDLDRLGRGDMKDQGIILETFKE
                     SKTKIITPRKTYDLTDEFDEEYSEFEAFMARKELKLISRRMQRGRIKSVEEGNFIGTS
                     APFGYDAVTTGRKERILVPNKDADVVRTIFDLYINEDMGCSKISKYLNNLGIKTATGA
                     NWYNSAITNIIKNKVYCGYIQWQKKDYKKSKNPNKIKTVKLRPKDEWIEAKGKHEPLI
                     SEITWKKAQNILKKNGHVSYGNQIKNPLAGIVICKNCGRPLVYRPYADHDYIICYHPG
                     CNKSSRFEFIEAAILKSLEDTVKKYQLKASDLDLDKNNKDSNIEFQKRVLKGLETELK
                     ELGKQKNKLYDLLERGIYDEDTFIERSNNISSRTEEIKDSIKTVKNRLSTVKKDNAKI
                     IEDIKTVLSLYHDSDSLGKNKLLKSVIDKAVYYKSKEQKLDSFELMVHLKLHED"
     gene            56199..56351
                     /locus_tag="ACGI59_000471"
     CDS             56199..56351
                     /locus_tag="ACGI59_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9738284.1"
                     /translation="MDNFLFNVLASLTASVVVYLISKLFKKAKSHSRTKSDLRVEFKF
                     IFKSKK"
     gene            56742..56918
                     /locus_tag="ACGI59_000472"
     CDS             56742..56918
                     /locus_tag="ACGI59_000472"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9738285.1"
                     /translation="MQQLNFIIFKRFILTKWYVKNDISGVVYIVMDSEGAWEFKIAKE
                     MEESEYQIDLNKIL"
     gene            complement(56975..57190)
                     /locus_tag="ACGI59_000473"
     CDS             complement(56975..57190)
                     /locus_tag="ACGI59_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFG9738286.1"
                     /translation="MLKDLRILRGLTQKELANRTNYSREYISDLERNKYKNITIPTIV
                     DLSIGLEISPLLLACHFIDEELKKRDK"
BASE COUNT        23106 a         5939 c        10563 g        17956 t
ORIGIN      
        1 tataatataa aaaaaaagta tatactttta tattaaaagg tgatatatta tttatgaaaa
       61 tcgtttaatg tatacaataa tatatctata acaaaataat aaaagataga gttttattta
      121 gacatttatt acaaatcaaa atttattaca tattatttat aaaaaaatct aaggaggaaa
      181 taataatgaa aatattagta ttaaactgtg ggagttcttc attaaaatat caattaatag
      241 atatgaataa tgaagaagtt ttatgtatag gattggttga aagaatagga atagaaggtt
      301 ctatattaaa acatgaaaaa gcaggtagag atgataaata cgttgttgaa caaccaatga
      361 aggaccacaa agatgcaata gcattagttt tagaagctgt tgctcatcca gaatttggtg
      421 cagttaaaga aatgaaagaa atagatgcag taggacatag ggttgtacat gctggagaaa
      481 aatttgcaac ttcagtagta ataactccag aagtagaaga agctttaaaa gaatgtatag
      541 atttagctcc acttcataat ccagcaaaca taatgggaat agatgcttgt aaagctatac
      601 ttccagatgt accaatggta ggtgtatttg atactgcttt ccatcaaaca atgcctaaat
      661 cttcatactt atatggttta cctcatgagc tatatactaa atatggggta agaagatatg
      721 gattccacgg aacttctcat aattatgtat ctcaaagagc agcagagata ttaggaaaag
      781 atataaaaga tttaaaaata gtaacttgtc acttaggaaa tggtgcttct atagctgctg
      841 ttgatggtgg aaaatgtgta gatacatcta tgggattcac tccattagaa ggattaataa
      901 tgggaactag atgtggagat atagacccag ctatattacc attcttaatg agaaaagaag
      961 gtttagatgc tgatggattg gataaattaa tgaataaaga atctggagta tatggaatga
     1021 ctggaatttc tagtgacttc agagatatag aagatgcagc taaaaatgga gacgaaagag
     1081 ctcaagctac attagaagcg tatgttaaaa aagtacaaaa atatataggc gcttatgctg
     1141 ctgaaatgaa tggattggat gttgtagtat ttactgcagg cgttggtgaa aatggaaagg
     1201 ctataagagc agatattgct tctaacatgg agtttttagg aatgaaatta gataaagaag
     1261 ctaatgatgt tagaggaaaa gaaacagtaa tatctactgc tgattctaaa gtaaaaatgc
     1321 ttttaatacc aactaatgaa gaattaatga tagctagaga tacattaaga ttggtaaaat
     1381 aattaaatta aacctatgcc tatcaagtaa aaatacttga caaacaaatg ctagatttgt
     1441 ataataaatt tggtgatatg ttgaggtgaa cttaatgaaa gttagcatag aaaaaataaa
     1501 tagaaaagaa actgacaaaa tagacttgaa tttttgtgaa aaaattgata ctattagtta
     1561 ttgtgatgag atttacaaat tagtatcacc tgtaaattta aaagggaaag tgtcaaagac
     1621 taataaaggt ttgtatctag atatagatgt caattttaca attgttgaca attgttcaag
     1681 atgtcttaaa gaagtagaaa taccactaga gtattctata caaggttttt tagtaaaaga
     1741 agaagattat gatgaagatg agtttgagga atttgatcct tttatattcg atggtgaaga
     1801 aatcgacctt attgatataa tagaacagac attagatttt aatgtacctc ataaagttct
     1861 ttgtagtgaa aactgtaaag gtctttgtca agtatgcggg gcaaacttaa atgaagaaga
     1921 gtgttcttgc agtgaaatta caaacgatga ggaatacata gatcctcgtt ttgctaaatt
     1981 aaaagattta tttaattaaa ttttgactaa ggaggtggcg taaatggcag taccaaagcg
     2041 taaaacgtct aaatcaaaca caaaaatgag aagagcagct aactcaaaga tggaagcaac
     2101 aggatttgta agttgcccac aatgtcatga gccaaaatta ccacatagag tgtgtccaga
     2161 ctgtggatat tataaaggta aagaagttgt gtctaagtaa tagcttctaa aaagcatgta
     2221 gagtatactc tacatgtttt ttttgtacat ataaaaaaat aagataattt actagagaaa
     2281 ataagtggat tttatgcata ttaaaaaata gaaaaataat aatataagta ttgaaaattt
     2341 ttgtgtgtta ctatatacta ctattagtag ctggtactaa ttacagctat aaaatttgga
     2401 gggaacatga aaaagaaaag taaagcccaa agacaaaaag agcttataga tatgctaaag
     2461 acagatcctt tttatactga tgaagaatta tcaagtctgt ttgatgtaag tatacagacc
     2521 ataagattag atagaatgag tctaaacata ccagagctaa gagaaagagt caaatcaata
     2581 gcagaaactc agagttcaaa agtaaagact ttaggagtaa aggaaattac aggagaaatc
     2641 attgatttat ctgtagggcg attaggtata tctatgttag aagttacaca ggacatgatt
     2701 tactctaaga ctaatacttt aaaagatacg tatatttttt ctctagcaga ttcattggca
     2761 atggctataa tagatgcacc taaggttatt atgagagttg caaatgtaaa aagttttaag
     2821 ttgatagaac aacaagatag gcttatagca aaagcagaag tgtatagaaa tatagataaa
     2881 aagcattatg taaaagttgt tatcaacaat aaagcccaag agcaaatatt tagaggaaag
     2941 tttatctttg aagaattaga ttaggaggga aattatgaaa atagtaattg atggaatggg
     3001 tggagataat gcacctaaat ctaatgtaga aggcgcagta aatgctataa aagaatatca
     3061 ggttgattta ataatcacag gagataaaga tttacttgaa aaggaatttt caaattatga
     3121 atttgataga aataaattag agatagttca tactactgag attatagaaa acgaagataa
     3181 accagtaaag gctataagaa gtaaaaaaga ttcatctatg gtagtagcat taaatcttgt
     3241 aaaagaagga aaggcagatg ctataatatc agctggaaat acaggagcct tacttgctgg
     3301 tggattattt gttgttggta gaataaaagg aatcgataga ccttgcttat gttcagcaat
     3361 accaaatgta aagcgaggaa tgacacttat agcagactgt ggcgcaaatg cagattgtaa
     3421 acctaagaat ctagttgaat ttgcagctat gagtaatatt tattcaagaa aagttttagg
     3481 tcttgaaaat cctaaggtag cattagcaaa tgttggacta gaagaaggca aaggcaatga
     3541 tttagtcaaa aggtcatatg aagaaattaa aaaattagat ttaaatttta tagggaatgt
     3601 agaagctaga gaagttataa atgcatacac agatataatc atatgtgatg gatttacagg
     3661 taatatatta cttaaatctg ctgaaggtgt agcactttca gttatgagtc ttataaagga
     3721 aacttttatg gccagtacaa aaagtaaaat aggagcatta cttataaagg atgatttaag
     3781 aaaattaaaa agttttatag attattctga gtatggggga gctccacttt taggtctaaa
     3841 tggaggagtt ataaaagctc atggtagttc tgatgctaaa gctataaaaa atgctataaa
     3901 tcaaggaata aaattttcaa agggaaaagt cgttgaagat attaaccaat ttataagcaa
     3961 atacaatgaa gaaaataaaa ataacgaaga tgaataaact cgttttggag gtttaatata
     4021 taatgaatac aaaggctgga atattaggag taggaagtta tctacctgag caatcatatg
     4081 ataattttca cttcgaaaaa ataatggata catcagatga gtggataagt actagaactg
     4141 gtattaagga aagaagattt gccaaagagt ctgaagcaac ttctgactta gcatctaaag
     4201 ctgccttaaa agctatagaa tgtgcaaagt taaacgtaga ggatatagag ctgattatat
     4261 tagctacaat tactcctgat atgtctttgc cttcaactgc atgtatagta caagatgcaa
     4321 taggagcagt aaatgctact gcatttgata tatcagcagc ttgttctgga tttgtatatg
     4381 gagtaactat tgcaaagcaa tttgtggaaa caggttgcta taaaaatgtg cttgtaatag
     4441 gagcagaaac ttgttctaag tttttaaatt atgatgatag aacaacagca gtattgtttg
     4501 gagatggagc tggagcagct gttattggcc ctgtaaatga gggtggaata ctgtctacac
     4561 atatgggctc tgatggtaaa ggaaaagatt gtttaaaagt acctgctgga ggttcaagac
     4621 ttaaggcaag taaagagact gtagaagcta atctacatac aatagaaatg gcaggtagtg
     4681 atgtgtttaa atttgcagtt agaaagatgg cagaaacttc tttaagagct cttgaaaaag
     4741 caaatctaaa tacaactgat atagactact tagtgccaca tcaagctaat ataagaatca
     4801 tacaagcttc ttctaagaga ttagagcttg atatgaaaaa agtttatgta aatatagaca
     4861 aatatggaaa tatgtctgct gcatctatac cagtagcatt agatgaagct tatagagaag
     4921 gtaaaattaa aaagggtgat aatgtagttt tagttggctt tggtggaggt ctaacttggg
     4981 gggcttcagt tgtcaagtgg actttatagt ataaaatatc taaaatgatt taattaacaa
     5041 aattaaactt atacacaaaa taaagttgat taataataaa aagtttactt ttatgatttc
     5101 aagtgaagga gaattgtatg aataaaattt gcaaaatatt aaatataaaa tacccagtta
     5161 tccaaggggg aatggcatgg gtagctactg catcattagc aagtgctgta tctaatgcag
     5221 gaggacttgg cataatagca gcaggaaatg caccaaaaga agctataaag aaagaaattg
     5281 ttgagtgtaa aaaattaaca gataagcctt ttggagtaaa tgtaatgctt atgtcgccat
     5341 ttgttgatga tataattgat ttgattatag aagaaaaagt tcaagttatt actactggtg
     5401 ctggaaatcc tgcaaagtat atggatagat taaaggaagc tggaacaaag gttattcctg
     5461 tagtacctac aatagctttg gcacaaagaa tggaaaagct aggagctaca gcagtaatag
     5521 cagaaggtac tgaaggtgga ggacatatag gagaacttac tactatggtc ttagttccac
     5581 aagttgctga tgctgtaaac atacctgtaa tagctgctgg aggaattgta gatggtagag
     5641 gaattgcagc atcatttgca ttaggtgcca gtgcagttca agtaggaact agatttattt
     5701 gcagtgaaga gtgttctgtc cattcaaact ataaaaactt agtactaaaa gcaaaagata
     5761 gagatgcaat tgtaacagga agaagtactg gtcatccagt aagaacatta aaaaataaac
     5821 tatcaaaaga atttttaaag atggaacaaa atggagctac tcctgaagaa ctggataaaa
     5881 aaggtacagg agctttaaga tttgcaacag tagatggaga catagaaaaa ggttcattta
     5941 tggcaggtca aagtgctgct atggtaaaag aaataacacc ttgtaaggaa attatagagg
     6001 ctatggtaaa tcaagcaaga gagattatgc cagcaataga actgtaaatc aggaggagca
     6061 agatgggaaa agttgcacta gtattccctg gtcaaggagc tcaatatgta ggtatggcca
     6121 aggatttata tgaaaataat gctgttgcta aaagtgtaat agatgaggct agtgatgcct
     6181 taaatatgga tttaaaaaac cttatgttta atggaaatga agaagaactt tctaaaactg
     6241 aaaacactca acctgctatg gttacacata gtgtagcagt attaaaagcg gtacaagctc
     6301 aaatagattt aaaatataat gcatgcttag gattatcctt aggagagtat agtgcgttgg
     6361 ttgcagcaga tgcaattgac tttaaagatg ctgtatgttt agttaaaaaa agaggtaagt
     6421 ttatgcagga aacagttcca gcaggtgttg gagctatggc agcagtttta ggtttagata
     6481 gaaatgtact tgaaaatgtg gttaaagata tacaaggagg tattgtagaa gtagcaaatt
     6541 acaattctcc tggacaaata gttatatctg gtgaaaatga aaagatagaa gaagcaatgg
     6601 taaaatgcaa agaagcagga gcaaaaaggg cagttaaatt aaatgtaagt ggaccttttc
     6661 actcatcaat gttaaaagaa gctggtgtaa aattggccag tgaattagaa aaagttaaca
     6721 taactaaacc taaagttgat gtggtggcaa atgtaaatgc agattactat aaaaacgaag
     6781 gtaaagacct gcttataaaa caagtaagtt catctgttct ttgggaagat tctatagaaa
     6841 gacttttaaa tgatggatat gatactttta tagaaatggg acctggaaaa actttaaaag
     6901 catttattaa gaaaatagct tccaataaaa aagcaaatgt caatatttat aatatagatg
     6961 gtatagattc tcttaatgaa tttgtacaaa attatagaaa tggagagata taatgataaa
     7021 tcttacagga caagtagcag ttgttactgg tggttctagg ggtataggaa aagaaatagc
     7081 aaaaaaacta gcatcttttg gagctgatgt agtaatcaat tatacttcta aagaagatga
     7141 agcactaaaa actaaaaatg aaatagaaag tatgggggta aagtgtacct ctataaaatg
     7201 tgatgtgtct aaatttgatg aagtgaatca aatgatagat tctgttgtaa gcgaatttgg
     7261 aaaaattgat atattggtta ataatgcagg cataactaaa gatggtctgc ttatgagaat
     7321 gaaagaagaa gattttgata gagttataga tataaactta aaaggtgtct ttaattgtac
     7381 aaaagcagtt actaaaccta tgatgaaaaa gaagtatgga agaataataa atatgacttc
     7441 agtagttgga attatgggta atgcagggca aactaattat tgtgcatcaa aagcaggtgt
     7501 aattggattt acaaaagctt ctgcaagaga gttagcatca agaaacataa atataaatgc
     7561 agtagcacct ggatttatag aaacagatat gactaaagta ctaagtgatg atgtaaaaga
     7621 atcaacacta gcaaacatac caaagaaatc ttatggtaaa ccagaagatg tagccaatgc
     7681 cgtagcattt ttagttagtg acatgtcaag ttatataaca ggacaagtaa taaatgtaga
     7741 tggtggaatg gtaatgcaat aataatttaa aaagtagaga aatctattaa aataaaaaat
     7801 tttaggaggt acacaatgtt taataaagtt gtagaaataa taatagagca attaggagta
     7861 gaagataaag aaataactat ggaaacatca ttaatgaagg atttagaagc agattcatta
     7921 gatgcagtag aaataataat ggcacttgaa gatgaatttg gtatagaaat accagataca
     7981 gaagcagaaa acttcaaatc tataggagat atagttaagt atatagaagc taataaatag
     8041 tcgagcttta aaaaagtgga ggtatccgat gaataaaaga gtagtaataa caggacttgg
     8101 atgtgttacg ccgttgggaa caggaaaaga agagttctgg agtaatataa aatctggagt
     8161 atctggtata gataaaataa ctaattttga tgctagcaca tatcaaactc agatagcagg
     8221 agaagttaag aattttcatc cagaagaata tataagtaaa aaggaattga agagattaga
     8281 taaatttgct caatttgcaa tagtatctgc taaattagca gttgaagatg caaatcttga
     8341 tttagataaa gttgatagag aacgttttgg tgttataata ggttctggaa taggtggagt
     8401 tgaagctata gaaactcaac ataaaattct tttggaaaaa ggaaataaaa gagtaagttc
     8461 actttttgta cctatgatga taggaaatat ggcagctggt caagtttcaa tatttttagg
     8521 agcaaaagga cctaacacta atgtatgtac agcttgtgca tcaggaactc attcaatagg
     8581 tgatgctttt aaggtaattc aaagaggtga tgcagacctt atggtagcag ggggttctga
     8641 agctgctgta acaggtcttg cttttgctgg attctgtaac atgaaggcta tgtcaactag
     8701 aaatgatgac cctaaaactg catcaagacc atttgataaa gacagagatg gttttgtaat
     8761 gggagaagga gcaggtattg ttatcttaga agacttagaa catgctttag caagaggtgc
     8821 taaaatatat gcagaagtag ttggatatgg tttaactgct gatgcatacc atatgacaac
     8881 tccagctgaa aatggagagg gagcagctag gtctatgaat atggctttga aagatggaaa
     8941 tgttccatta gaagaagtag attatataaa tgctcatgga acttcaactt attacaatga
     9001 tttatatgaa actatggcta taaaaactgt atttggagaa aaggcatatg acttatgtgt
     9061 gtcatcaaca aaatctatga ctggtcactt attaggagca tcaggtgcta tagaggctgt
     9121 tgtttgtgct atgagtatag aagatagttt tgtgcctcca actataaaca tacaagaagt
     9181 tggtgaagat ttggatttag attatgttcc aaatcaaggt aaagaaaaaa atattagata
     9241 tgcactatca aattctttag gatttggtgg gcataatgct acaatagtac taaaaaaata
     9301 tgtataaata aatattaaat ataaaactaa aaaacaagca cttgttttaa ctgtataaat
     9361 ttatattagc ttaaatttaa agtctcctaa attattttag gagatttttt tattaagatt
     9421 taagtctagg atatgttgta aatcattttg atatattgta ttattaatta ttaagaggtt
     9481 atttatatat gcaagttttc attctacaat atacaaaagg aggactaaaa ttttgaattt
     9541 tagactacca aagaaaaggt tacccaaaaa gataattttt atatgtataa tacttattag
     9601 tattattttg tttataaagt atgaccaaaa tggattgaaa gaaagagaaa ttataaataa
     9661 gggaaaatat atgtctgaaa gccttgtggc agataaggag tatgatgaag caaaaaaagt
     9721 ggttaagact tcttttgaat ctattccaga aaaaaaatac aataaagtat ataatgaagt
     9781 ttgggatatg ttaaagacta tttcatatgt tccagatgga acgaagatag tcattgattc
     9841 tttagaaaag atgagagaat cagatgtgaa attatctgat gaatgtagat ttaatataga
     9901 aaaaagatta gcatcagtgt atataatgga taataattat tctaaggcag tagatttaac
     9961 tgtaaagtct ataaagttgg ctgagaaatt ggggaataat tatgaaaaag caagactaga
    10021 tgttgatttg gcgagcatat ttttaaagat aggtggctat gaaacaggag agaaattaat
    10081 aaaggattct tttaaaatag atattgatga aggtgaaaaa aatggaatgg ttagattata
    10141 tgctattata aatctagcag aaatatatgt agaagagggc gaatatgata aagctattga
    10201 aataagtagc aggataaaag attataaaaa gtttgtaaat acggataatt acaatgattt
    10261 tgatataatg gcctctataa tgcagtcaaa tgcgtatgtg ggcaaaaata atatacaaaa
    10321 ggcaaagact tttttagaaa aagctgatta tcttataaaa tcagatagaa cagtttatat
    10381 attagataaa gatatgtact attatatggc aatgggaaac ttagaatata aaagcgaaaa
    10441 ttataattct gcaatagata attataaaaa aagtttagag atatctacta aaagaaagtt
    10501 aacccaggag aaaataaaaa atttaaataa gatattaaat atatatgaaa aacaaggaaa
    10561 tatagaatct ttaaatgagt atcaaaagat gttaataaat gaatataaag agaacaaaag
    10621 tataagagat tcagagacta gtttctatat aattgataaa gtatcaaacg aaagtaagct
    10681 atttgaaaaa actaaaaaag aaataaaata ttataaaata ttatttacag taattttaat
    10741 tgctatatta gggtctactt ttttatacaa ccgattgaag tattttaaag cacaaaattt
    10801 acatgatggt cttacaaatg tttataatag aaaaagtttt gatattatgt atgaaaagta
    10861 cagagagaaa gatgataatt ttgcacttgt gatgatagat attgataatt tcaagctcat
    10921 aaatgataat tatggacata aatttggtga cactgttata aagggaataa caaataccat
    10981 ttgtgttatg ttagaacaag atgataaagt ctttaggtac ggaggagaag aattttctgt
    11041 attaataaga aataaatctg gtgatgaagt agcagatata attgacagta taagggttgc
    11101 aattgcaaat aaaaagtgga atgatgatgt gactgtgact ataagtgcag gtgtagctca
    11161 tgtttcagat agcaaagatg tactagaaga agctgataaa aatttatata aagcaaaaca
    11221 acttggtaga aataaagtag tatataagta attatatact atataaataa gaggtataaa
    11281 tatgaaacaa tcaatagcta tagtaactga taaaataact aaactatcat ctttcttaga
    11341 agaaaatata cgtttggttt taggtaatta tattgatata aaccactatt atctggaagt
    11401 tttaaaagag aatgataaaa tacaaggtga ttttgtacta gtaatgaatg atgatagatt
    11461 aaacagtatg agaaagcatt tagtacaaga tagtaagatt atagtgataa ggagaacact
    11521 taaagaaaat gaaatatatg gattattttc aataccaaat ggaactgaag tattggttgt
    11581 aaatgataca aaagaaacta ccttagaaac gataagctta ttttacaaaa taggagtaaa
    11641 aaatttaaag tttactccat atattgaagg aaaaatttat aaaaatatag acatagctat
    11701 aacacctgga gtaaagggaa aagtaccaag ttatataaat aaagtaatcg accttggaaa
    11761 tagatatatt gatatatcta cctttataga aataataaat ggtcttagaa tagatgttaa
    11821 agaaatacgt aaaaatttaa tggcatattc tgagagttta ataagtttag ataatggagt
    11881 taaagataat tataggcaac tgtttttaaa aatagaagaa caagatgtta tattgaatct
    11941 atcaaaagat ggaataatat ttacttcagc agatggtata ataaacacat ttaataaaga
    12001 agctttaaaa attttaggta taaatgaaaa tccacaaggc aaaaacatag aagatatcat
    12061 ttctgaagat ttaaatattt tacttgaaga caaagaaata attgatgaag tggtatttac
    12121 aaataaaaaa tatataaatg taaataaaaa aagtattttt tctatgggga ataaagctgg
    12181 gatatattat aacttacaag aaataacgta tataaaaaaa ttagaacaaa atcttactaa
    12241 taaattaaga gaacaaggtc agatagcaag atatacattt aaagatataa aaactaaaaa
    12301 taaaaatatg gtaaagtgtg tagaattggc taaaaaaata tctaaatcag acctttctgt
    12361 attaataata ggagaaagtg gtactggtaa agaattatta tcacaatcaa ttcataatga
    12421 ttccaataga agtaatcaac cgtttatagc agttaattgt gcagcagttc cagatagtct
    12481 attagaaagt caactgtttg gatatgaaaa aggctctttt acaggagctt taagagaagg
    12541 aaaaaagggt ctatttgaac ttgctaataa tggaactata tttctggatg aaattggaga
    12601 tatgcctcca ttgctacaga caaagttact aagggtatta caagaaaagc aggttatgcc
    12661 tataggttcc cataatgtta taaacataga tgttagggtc atagcagcaa caaacaaaaa
    12721 cttactaaag atgattagag aaggaaaatt tagagaggac ctgtattata gactaaatgt
    12781 attgccaata gatgtaccat cattgaggaa tagaaaagaa gatataataa cacttatgaa
    12841 tttctttttg aaaaacaata taagactttc gccagaagtt ataactattt tagaaaaata
    12901 taattggcca ggaaatataa gagagttaca aaatgttgct tcttatgtaa gtctaatgtg
    12961 tgatagtatg gtttttagag atgacttacc accatatata agggtagaat gtgctaacaa
    13021 ttatgaagat ggatttggtt atacaagaaa agaatttgag gaaatactga ggattctata
    13081 tgaaagaaga gagtcatcct ctggaattgg aagagggttt ataatcgaaa agcttataca
    13141 ggaaggtatt gagatttctg aaagcaaagt tagaaaggtg ctatcttatt taagtgaatg
    13201 tggatacata tcatctaaat ctggtagagg aggaagtcaa ataacctcag aggggataaa
    13261 actttataat aatataaatt aagctaagta tttgatattg aaatcacata ttttgaattt
    13321 agatatttaa ataataataa acttttatat ttatagagat taatttatat tatgtcagaa
    13381 agaatattgt aatttataat taaatattaa taggttttta aaataggatt agataggtaa
    13441 taggttaagt tagaataacc cattacctat ttttatttgt aagaaaagtg tacatagaat
    13501 tagaagtaaa aaatacaact aataaaacta ttgaaaatac tagataaata ataaataaaa
    13561 ataattatag attttggcat acatattgct aattatagag ataagacgac aaaataattg
    13621 ggaggtttaa tgatgaaata caaaacaaca gggacttgtg ctacagaaat tgaatttgaa
    13681 gtaaaagaaa ataaagttac aaatgtaaac tttataggtg gatgtgatgg aaatttaaaa
    13741 gggctaaaag tattagttga aggcatgaat atagaagatg taatacaaaa gttaaaagga
    13801 atagagtgta aaacaaagcc aacatcttgc ccagaccaac tgtctctagc attggaaaat
    13861 tatatgaata taaagtaata cataaaacaa tcgattagtt aggagtttaa ttatgaaacc
    13921 aataataggg attttaggaa atttaataat aatggaaaat ggtatgtttc caggtcttga
    13981 aaggtcatat gtaaataatg attatataaa tgcagtgcta aaaggtggcg gtagtccagt
    14041 aataattcct gtaaatacag ataaagaagt tataaaaaaa cagattgaaa tggttgatgg
    14101 agtcttaata tctgggggat gggatatcaa tcctcaactg tatggagaag aaacaagaga
    14161 agaaacgaca tttatatatc cagaagtgga tgaatttgat ttaattgcaa taagtatagc
    14221 attagaactt aaaaagccta tattaggtgt atgtagggga ctacaaattt taaatgtaag
    14281 tttaggagga actttatatc aagataataa tcttatagaa ggaagttata taaaacatac
    14341 acaatcttct aaaagacatg tagctactca taaagttgat gttaaagaag gaagtatttt
    14401 agagggaatt ttaggaaaac aattacttac aaatagttat catcatcaaa gtgtcaatca
    14461 gcttggaaaa ggattaaaag ccatagcata ctcaaaagat ggtataatag aagctattga
    14521 gaaagaggat gaaaattttg tagtaggagt acagtggcat ccagaaatga tggtagatta
    14581 ctgtgataaa atggaaaaac tatttaaata ttttataaat atttgttcga atttataata
    14641 taccttgggg ggattgaaca tgaacgaaaa gaacaaaatg ggattaataa gtattatact
    14701 tttaggtatt aatgcagtag taggagctgg agtttttcta ttaccaggag atgcaatgaa
    14761 atcatttggt gtagcaagta tatttgtata tatatttgat atgttgttag ttttatctat
    14821 ggcattctgt tttgctgagg ttgcaggtaa attcaataaa aatggtgctg cctatgtata
    14881 tacaaaagaa gcttttggag atttttgtgg ttttgaagta ggtcttatga aatgggtaat
    14941 aggatgtatt tcttggggag ctttaatagt aggttttcct acatcattat cagcagtatg
    15001 ggctccagca ggtgaaccac atatacaaaa aattataatt gtagctatga tagttgggct
    15061 tacaattata aacttattag gtgtatctct atcaaaaatt gtacaaaatg ttataactgt
    15121 aggtaaacta attccattaa tactatttat aggaataggt atatttttta taaaaggagt
    15181 taattttact tcttcaacaa tggtacctcc aggagctgga gcaacagagt ttggagcagc
    15241 agcactttta atgttttatt catttacagg atttgaatct atagcagttg cagcagaaga
    15301 tatggaaaat ccacaaaaaa atataccaat agcaattata agtgtaatag taattgcatc
    15361 aataatatat atattaaatc aagttgtatg tgttggtata ctaggagatt cgttatctag
    15421 tacatctaca ccagtggcag atgctgcaag aatatgcttt ggaaatatgg gagctggatt
    15481 ggttaccttt ggaacattgg tttctgttgg tggaatttgt atgtgtggtg cttttgtaaa
    15541 tccaagaagt tgtgtagctt tagctgatga taaaatgtta ccgagaatat ttgcaagaaa
    15601 agataaaaaa ggtacaccat atgtagcgat aatagctact atgttaatca ctataccaat
    15661 agctctatct ggaagttttg ctgaattagc agcaataagt gcagttgcta gattcataca
    15721 atacatacca acatctttat ccgtattagt gtttagaaag aaaagaccag aattagtagg
    15781 tacttttaaa actccttttg gagcagtgat tcctctaatt gctgtatgtg ttggaacatg
    15841 gttgttattc caagcatcta tgcatcaatt aattatggga ttaggagctt tggcaattgg
    15901 agtaccatta tactttataa tgaagtctta taatagaaaa atctatggtg aaaatttaaa
    15961 gaaaatagtt taattatgtt ataacaattt attgagttaa aattagacat tggcagaaaa
    16021 ttaaatacaa ataagtatct acaaggctat attaaaatat tattaatttt ttattttaat
    16081 atagccttat attgttatca ataaatgaaa aatattgtaa caacagaaga acaatagtat
    16141 tatgtttata gacaaaaaac ttattaggag gtaaatttat gccaacaata acattaaaaa
    16201 atggtgtaga cgtacttatt agagaaggtg taagagaaga tgctcaaagc attatagatt
    16261 tttataatga agtgggtgga gaaactcatt ttctgtcatt tggaaaggat gaatataaaa
    16321 tatctttgga ggaacaagaa aatgctatag aatcagcaaa agcatctgat aattctgtta
    16381 aactgattgc atttatagat ggtgaaatag ttgggattgc aacgattgat tctaatcaaa
    16441 aagcaaaagg aaagcatgtt ggagtattag gaattgttgt aaaagaaaaa tattggggaa
    16501 ttggattggg taaaaggctt atgttagacc ttatagaatg gtgtaaatca aatggaataa
    16561 ccaagaagat aactcttgtc actaatgaag aaaattataa tgctatagga ttatacaaaa
    16621 aagttggatt tgaagtagaa agcatactaa agaaagaatg ttattataat ggtgtttata
    16681 ctgatttaat aggaatgtca cttttattgg gaatataatt agtgtacatt ttatctcgaa
    16741 aaaagtatat taagtatggt ataattaaaa aagaatataa cttaatataa ttttggagga
    16801 cgaattatga aaaatctgtg taaaatatca gatgattatt taaacagcaa gcttaagtat
    16861 ttaaagttat tatcaaaaca atatccaagt atatcaaaag caagtacaga aataataaat
    16921 ttagaggcta tattaaatct tccaaaggga acagagcatt ttataacaga tgtacatggt
    16981 gaatatgaac catttgtaca tgtattaaaa aatggttctg gtgtaataaa gagaaaaata
    17041 gaagaattgt tttcaaatac aataagagac agcgaaaaaa aaatgttagc aactcttgta
    17101 tattatcctg aacaaaagtt agacctaata attaagcaag aggaaaatat agatgatttt
    17161 tatagaataa atatttatag acttatagaa ctttgtaaat atgcttctag caaatatact
    17221 aggtctaaag ttagaaaact gttaccagaa aattttaaat acataataga agaacttttg
    17281 catgagcatg ttaaaagtga acataaagaa gagtattata aaagtatagt tgaaacaata
    17341 gttgacatag gaatagctaa ggagtttata attgctattt ctacagttat acaaaaattg
    17401 gttgtagata gacttcatgt aataggagat atttatgata gaggtccaag accagatata
    17461 attgtcgata agcttattga acatcattgt gtagatattc aatggggaaa tcatgatata
    17521 ttgtggatgg gtgcagcctc aggtgaaaaa acttgtatag caaatgcact gaggatatca
    17581 gctagatatg cgaatttaga cattgtagaa gacatatatg gaataaatct attgccttta
    17641 gccacttttg ctatagaaat gtataaagat gacccttgta aagaatttat tccaaaggtt
    17701 aatgaccaaa gtgtaacaac aacagaaaag tctttaatgg caaaaatgca caaagcaata
    17761 agtataattc aatttaagct tgaaggtgag gttatcagac gaagacctga atttgaaatg
    17821 gaacatagac tcttgcttaa tatgataaac tatgatgaag gtacgattaa tttaaaaggt
    17881 aaaacatata aattaaaaga tacttattta cctacaatag acaaaaaaga cccatataaa
    17941 ttaactatgg aagaaagaaa tgttattgat aaattagtat cttcatttag aggtagtgaa
    18001 aaactacaaa aacatgtttc atttttgttt tctaaaggaa gtatatattt aaaggctaac
    18061 tcaaatttac tgattcatgg atgtgtaccg ttaaatgaag atggaagctt tatgtctatg
    18121 aatataatgg gaaaagagta taaaggtaag gctcttatgg ataagatgga gtctctagca
    18181 agagaaggat ttttctttaa agataaggca gaagaaaaac tgtatggcat ggatattatg
    18241 tggtatttat ggactgggaa atgttcatca ttatttggta aagatgatat gactacattt
    18301 gaaagatact ttatagctga aaaagagact cataaagaaa ataagaaccc atattttaaa
    18361 cttagagaaa atgagatggc ttgtaaaaga ttatttgaag aatttgattt agaacttgat
    18421 gaatctcata taataaatgg tcatgttcct gtggagagta aaaatggaga gagcccaata
    18481 aaagctaatg gtaaaattct cgttatagat ggtggttttt ctagggctta ccaaaaaact
    18541 actggtatag ctggatacac tttgatatac aattctagaa ctttgcaatt agtatcgcat
    18601 gaaccattta attctgctga agaagccatt gcaaatgaaa gtgatatctt atcgacaact
    18661 gttgtagtag agcataaggc aaagagaaaa atggtgagag atactgatga aggaataaag
    18721 atacaagagg aaatagaaga tttaaaacta cttttaatgg cttataagaa agggctaata
    18781 aaagaaatgt agatgttata gaggcacttc atgattgaag tgtctttttg ttaatactta
    18841 ttaaatatat tataaagatt aataatagta gtttatatta tacttactaa gtgaatccaa
    18901 gataaatggg ttcacttttt ttatttataa acattattaa aaaaatatag atgttttggt
    18961 tatattcctt gtcctttata aatagaatta tataaacaaa ggaggaggac aagagatgaa
    19021 taaactttct gatgaaataa taaactctct ttctacaact ataagagaaa aggtagaaaa
    19081 agtctcaaac aataatctga acatagaaga aattaggtta cgctcacaaa aaccattgat
    19141 actaaatgca aactctaaag attattttta caatcaaaaa actatgacac ttgatttaaa
    19201 tcaacaaaac tcatatgtag taacaagaga agatgtagag caaacttttc aaataatatg
    19261 taaatattca atacattcat ttatggatga cataaaaaaa ggattcataa ctttgagagg
    19321 aggtcataga gtagggttag ttggaaaagc aatagtagaa gatggacaag tcaaaaatat
    19381 aaagcatata tcatctttaa atataagggt atcaagggaa attattggat gctcggataa
    19441 gattttaagt catatcataa agggaaaaaa tcagattaat aatactttga taatatcacc
    19501 acctcaatgt ggaaaaacaa ctttaataag agatatagta agaaatttaa gtaatggtaa
    19561 tgaagattat ggatttaaag gtttaaaagt ggctttagta gatgagcgta atgagatagc
    19621 aggagcttat ctaggtgttc cacaaatgga tgtgggaata aggactgata taatagaaac
    19681 ttgcccaaaa gacttgggta ttacaatgct tttaaggtct atgtcaccaa atgttattgt
    19741 gacagatgaa attggaagtg aaaaagaaat aaaggcatta tacacagcat taaatggagg
    19801 tataggatta ataactactg tgcatggaga ttcaatagag gatatacaaa atagaaaaga
    19861 attaaataga cttctggata aagagttatt taaaaaggtt ataattcttt ctgcaaaaag
    19921 aggagcagga acaattgaaa aaatttatga tttagaagaa aagagatggt attttgcaaa
    19981 ttaaaataat tattatagct ttcttgatag gaagtagtta tttgataggt gaacaaatat
    20041 acaaaactta tactcgtaga cataaacaat taaacgattt aataagagtt ttagaaattt
    20101 tgaggatgga tttatctttt ggactttata cattagaaga aatctttaat aggataggtg
    20161 gaaataaaga gttttgtttt tggaaatttt tctatcaaat atcagaagga cttcacaatg
    20221 aacaaagtaa aactttggaa ataataatat cagaaaatat tgatgtctta agtaaagaaa
    20281 cttatctagg caataaagaa attgaggaac tgaaaaatct aatacttact ttagggaaaa
    20341 gcgatataga gtcacaacaa agaatgatag atttatctat agaaaatttg aaaaaacaaa
    20401 cctatgagac aaaggaagat ataaacaaaa aaggcgtatt gtataaaaaa ttagtgacat
    20461 tcataggtat aggtatatgt attatattga tttaatatgg ggaggtttat tatggaaata
    20521 tcattgatat taaaggttgc aggtgtcggt attctaatat cagtattgaa tatgatattg
    20581 gaaaaaacag atagaaaaga ttgggcaggt cttactacat tagcaggtgt aataatcgta
    20641 ttgggtatgg taataacaga aataagtgac ttgtttaata ctgtaagaac aatgtttcaa
    20701 ctttactaaa gaggagggag taatttggaa ataatgcaat taataggaat tgcaatcatt
    20761 tcaactacac tctgtttagt aattaaaaaa gatagaccag agatagcaaa tttcatagct
    20821 ataataactg gagtaataat actattgtca gttatgttta agctaaattt tatagtagat
    20881 agtatacaag atttggctaa caaggctaac atcccaacta tgtatatatc attaataata
    20941 aaattaattg ggatagcata cttaatggaa tttgcgatac aactttgtaa agattgtggg
    21001 gaaggaaaca ttgcttcaaa actagaattt ggaggtaaaa taatagttat gtcaatgtct
    21061 ttccctatac ttttgtccat tgtagaaatg gtggtgaata taattccata gatgtgtatc
    21121 ttggaatgaa tataagtata ttagagagct ttcgttgtat tgaggtttat agagtgaaga
    21181 gaggcatggt tgcttatgaa aaaaagattt ttaccaatga ttataggttt tttatttact
    21241 ttcttttttg taaatatctt tgctattatg atatttgcta atgaagtacc aagtagtgaa
    21301 gataaagaat atggtgaaac aaaaaatagc atagataagt atatagatgg tcaattagac
    21361 aagctagata taaatgagat tcaagactat ataaataaag aaatagttat taatgatgtt
    21421 aatttaaaat catttgtaaa agatttaatt agtggagaaa aaaatatctt agatttattc
    21481 aataaagatg gattgaagat attgatgttt gatgaattta aagcaagttt aaaggtagta
    21541 gcagtaattt tggtactggc tctgttatca tctattttaa agagtctgga aaactctttt
    21601 tcatcaggtg cagtaagtca aatagcaaca tatataatat ttatcacaat ggtatcactt
    21661 acacttattg gttttaaaga tgtattacag atttgctatg atgcaataga ccatactgta
    21721 ggtcttatgc aagttataat gccaatatta ataacatttt tacttttaat tggattccca
    21781 ataacgtcga ctactttgaa tcctatcttc ataggaggag taacatttat aaatgtattt
    21841 tttaaaaatt ttctatttgt atctattact gttgcatttg gaattttaat aataaacaat
    21901 ttatcaaaaa atataaggct aaagagattt ttctcctttg taaaacaaat aaattatgtt
    21961 tctattggag ctatgtttac tgtttactta ggtcttgtat ccatacaagg attgtatgta
    22021 accagttttg ataaattcag tgtaaagact gctaaatttg ctataggtaa ttttattcct
    22081 gttgtaggtg gatttgtatc tgactctgtg gatattttat tatcttcatc tcagcttata
    22141 aaaaatatat ttggaggaat aggacttatc ttacttgttg gaatttgctt acttccagta
    22201 ataaaaattt tatcagtaat agtagtgtac aaattagcag caataattgt agagcctgtt
    22261 ggagaggatg gaatatctaa ttttttaaat gaagtggcaa atttaatgat aataatgcta
    22321 gcatcagtaa ttgctataac agtaatgttc tttgtaactg tagctatctt gacctcgata
    22381 agtgtcgtaa gtcaagggta atttatatat cagtgaaaaa taattacatt taatctaatg
    22441 tttttagaga ggaggatgct atgttagagg gcataaaggc atggatagtt agtgttttga
    22501 taggagcatt tatagtaaat atagtagata tgattttacc aagttctaag ataaaacctt
    22561 atgtcaacct agttttaaac tttatgtttg tatttattgt aataactcca gtagtaggat
    22621 ttttttcaaa agatatgagt ttagaagata gaatattaaa atctatggga aactacaaca
    22681 agcagtatgt agatagtaca aatgctttgg caaaagagac tggaaataat agtctatcca
    22741 agggatatga ggatgggtta aaagaagtac ttaaattaaa acttgatgaa tatggttatg
    22801 atttagaaga tatagagctt aatggggcaa atataaataa tataaaaata aaagaaaaga
    22861 ataatagtgc aaaaagtaat aacagttcca atgaagaaaa taaaaataat agtactaaaa
    22921 gagaggaaga aaacataaac tctaatgaca aagaaaattc taaacaagtc ttcaaaaaag
    22981 gtacagagta tgggttgaac ctaaatgaag aaaaattaaa aaacgactta ataaaggtgc
    23041 tggatgtttc tatagaagat atacaaattg ataaatagga gttgggagaa tgttgaaaaa
    23101 cttaaatgac aaagataaga gaaaggtata ttcactaata actatagcag gaatatgtgt
    23161 ggtttcatta gtattattat cttgttttcc tagtagcaaa agcgataaac aagttggaaa
    23221 aacagagact aataaaaatg ctgaaaagca agtcactaaa gaacaagaaa aagatgattt
    23281 agagtcaaaa ttaacagcaa tactatctaa aattgatggt gcaggagacg ttgatgtaat
    23341 ggttacattt gaatcaagtg aagaaataca acctgctttt aattcaaata atacaacaga
    23401 aacaacagag gaaaaagatg cacaaggtgg agaaagaaca gttacgactt ctagtgaaaa
    23461 taaaacaatg ataacttcaa attcaagtga ccctgtagtt ataaaaacta cagagccaaa
    23521 gataaaggga gtgatagtgg tagcaagtgg agcaagtgac ccaagtgtta aagagacact
    23581 ttatagtgca gtacaaacat cattacaagt agctggtcat caagtagaaa tatattctaa
    23641 ataaatacat ataaccgggg ggataaatga atgaagttta attataaggg aagaggattt
    23701 gtaataataa ctttaactgc aatgttagtg gtagtaggaa cagtgaatta tcaactaagt
    23761 aagaaatctt tattagaaac atcaaaagag tttaaggcat atgaagaagc acagttgcaa
    23821 aaaaatactg atgagagtga tagtagtaag acagaggatt ctagtaatgt agataaacaa
    23881 gatggtaagg aaagtgctga tattgatata gttgatagca aagctagtaa agtaaaagaa
    23941 aaggcaactg agacaagtaa ggaaataaaa gctcaattat catcagagaa aaatatgaaa
    24001 aaggcatctt atattttaga tatgaaaatg aatagagaaa aacaaagaaa tgagttagtt
    24061 caagatttaa atgaaatgat aaataatcca tctacaacag aagaatctag aaaagaagct
    24121 tctaatatga aattaaatat agttaaaatt caagaaaaag aacttcaaat agaaaacctt
    24181 ttgagtacaa aagggtatga agaagcgtta gtttatataa gcgataacaa agttaatgta
    24241 gttgtaaatg aagctaaatt agaaaagaaa gatgcagcaa aaatatttga tttagttgca
    24301 gaacaagcca atgtgaagta tgaaaatatt aaacttacaa ataataatag taataagtaa
    24361 agtataaacc tcagaattaa ctgaggttat tttcttgcaa agtagacttg tatttggtat
    24421 tattatactt ataaaaatag ttccttaaaa tcaggaggta agttttaata tggaagataa
    24481 taaatttgga caagtaaaaa tatctaatga tgtaatagct acaatagctg gtttagcagc
    24541 attagaagtt gaaggcatag aaactacagc aacattaaca gataagttgt taaaaaataa
    24601 tggagtaaaa atacaaatcg aagaagagga tgttaattta gatgtaatgg ttacgataaa
    24661 gtatggaatg tcaataccag atacagcttt taaagttcaa gaaaatgtaa aaaatactgt
    24721 tgagacaatg acaggactga aggtttctca agtaaatata cacattcaag gaatcagctt
    24781 taagaaggat aaagttgata aagaagaagc aaaagcaact aagaaaaatt aatattagcc
    24841 ctctgaaaag tcagagggtt ttttgctggt aattgatttt atatggataa ttaaacttaa
    24901 aggagaattt tgattgatga aaaaagatag ggcacaaaag agtacaacaa gagaatatat
    24961 aatgaaactt atataccaaa taaatattaa taaagaagat tttgaaactt tagaagataa
    25021 agtagataat tttttaaaag ataattcaga acatattata aatagatata aagaacttgc
    25081 acttcaatac tcaaaaaata caaatttaaa gttagaagat actgaaattg aagatgttat
    25141 agacaaaaaa tatataaata cagtatgcaa agcattgaaa gaaaatcatg acaaaataga
    25201 tgagttaata aataagcatg ctaaaaactg gacagtagat agaatgccaa aggtagatgt
    25261 ttctatactt agattgtctg tgtgtgaaat actttattta gacactccta ataaagtatc
    25321 aataaatgaa gcagtagaac ttgcaaaaat atattgtgat gataagtctc ctaaatttat
    25381 aaatggtata ttgggaagtg ttgttgatga aattgggaaa taatataata attggaattg
    25441 atactagctg ctatactact tctatagcag ctatctcttt agataagaaa gttatattca
    25501 atgaaaaaat aatgttagaa gtaagggata attcaaaagg tttaaggcaa agtgaagctg
    25561 tatttcaaca tatcaataat ttagggattc ttagtgatag gataaaatct tttaaagata
    25621 agtttaatgt agaaggagtt tgctcatcaa aaaaaccgag acctgttgaa aactcatata
    25681 tgcctgtatt caatgtaggt cataattttg gaaaattatt gtctagtata tatggttgta
    25741 ggttttatga gactactcat caagagaatc atattgaagc aagtttatta aatagtaaat
    25801 taaaaaataa taataaattt atttctgttc atatgtctgg tggtactaca gagatacttt
    25861 taacaagcaa acaagatagt catcacaatg tatgtgatac taatttaggt aaaattgcca
    25921 aaataagtat taaaaaagat gataaaagta agttatacaa taattttgga tataatatag
    25981 atataattgg aggaagtaaa gatattagct ttggtcaact tatagataga gtaggaatta
    26041 agcttggata taaatttcct tcaggaaagt atttggatga aaatgcttta aattgtaatc
    26101 ttaaaataga aagtggtctg aaaacttctg taagagatgg gtatatgaat ttatcaggat
    26161 tagaaaatca ggtaaataag attataaatg ataatggaga taatactaat caaaaagaat
    26221 atatatctaa attggtatta gattcagtag ttagaaatat gtttaaatct ttagtgtatt
    26281 tatgtgaaac ttataatgta aatgaagtag tatttgctgg tggagtttct gcaagtaagt
    26341 atattcttag agaattaagc atgaagttaa gaaaaaagca tatagaagct tattttacag
    26401 aaccacaata ttcaactgat aatgctgtcg ggtgtgctat aataggattg aataactttt
    26461 taggagaaag agtatgaaac taagagcttt agatataagt gaagcaaact catatattaa
    26521 aagaatttta ataaacgacc caatactttc taatcttaaa gttaaaggag aaatttctaa
    26581 ttttaaggtt catagtagtg ggaatgtata tttatcactt aaagatgaaa catcaaagtt
    26641 aaactgtgta atttttaaaa gtaattttaa tcgcaattta aaattagata atggtgtaaa
    26701 aattatagct aatggatata tatcagtcta tgaaagagat ggtgcgtatc aattatacat
    26761 taatgaaatt gaaatagagg gaataggaaa tttacacata gagtttaata gattaaagga
    26821 aaaacttaat aaagaaggat tatttgaccc taaatacaag atacctatac ctaaaatgcc
    26881 aaactcaatt ggcgttataa catctccaac aggtgctgtt ataagagata taataaatgt
    26941 aataaaaaga agatatccga aagttaatat aaagctttat ccagtaacgg ttcaaggaga
    27001 taaatcagct gaagaaatct gtgaagcaat tagatttttc aatcatatga agaatgttga
    27061 tacacttata gttggtagag gtgggggttc gatagaagaa ctttggtctt ttaatgatga
    27121 aatggtagca agagaggttt ttaattctca aattccgata atatcagcag ttgggcatga
    27181 aacggatttt actatttgtg actttgtgtc tgatatgaga gccccaacgc catcagcagc
    27241 agctgagatt gctactccat cattggatga tataaactat aaacttggaa acataaaaag
    27301 taggatgagt aagtctctta caaatcaaat agagctagac caatataggt tagaaacagt
    27361 atttaataag ataaataact atttagattc gtacacaata aaagataaag ttatacaatt
    27421 agacaaaata tatgataaaa taatttttgg aatagaaaat aatttaaaat tagaagatga
    27481 aaaattagta aaaattggag cattactcca taatttaagt ccattagcta ctatggatag
    27541 aggttatagt ataactcaaa aaaatggaaa agtaataaat agtataaaag gattaaaaat
    27601 aaaggatagt atagatatag tattaaaaga tggaaattta gaatgtatga tagataaaat
    27661 tgaaaataaa gagggatagt atgaatttaa cctacgaaga agcttataaa agattagaaa
    27721 gtattttaag tgaattagaa tctaaaaatg ctagcttgga tgaatcttta agcctatatg
    27781 aagaaggtat aagcctttat aaacattgta ataagctttt agatgatgct aaattgaaaa
    27841 taagtaaatt taatcaattg ggaatagaag aagactttaa aatagaggag gaataaaatt
    27901 ggaatttaaa caatgtctaa aagaaaaagc aagttttgta gaaaaagtac ttaaagaata
    27961 tatgcctaaa gaagaagggt atcaaaaaac tgttattgaa gctatgaact atagcttaag
    28021 tgctggaggt aaaagattaa gaccaatact tacattggag gcttgtaaaa tagttggagg
    28081 taatgaagat gaagctattc cttttgctat tgcaattgaa atgatacata catattcact
    28141 aatacatgat gatttgccag ctcttgacaa tgatgattta agaaggggaa gaccaacaaa
    28201 tcataaagta tatggagaag ctatggggat tttagcagga gatgctttgt taaactatgc
    28261 ttttgaagta atgcttgcag gctcaatcaa taaagaaaat ccagaaaagt atctaaaagc
    28321 tataaatgaa attgccaaag gtgctggtat atatggaatg ataggtggtc aagtagtaga
    28381 tgtagaaagc gaaaataaac aaattggaaa agaaaaatta gattatatac atatgaataa
    28441 aactgctgca atgatggtag gctgtatgag agcaggagct actataggtg gagcaaattc
    28501 tgaacaaatg gaagaaataa ctaaatatgc aaaaaatatc ggattatcat tccagatagt
    28561 agatgatata ctagacatag taggagatga agctaagtta ggtaaaaagg ttggaagtga
    28621 tatagaaaat cataagtcaa catacccatc tcttttaggt ttagataaat ctaaagaaat
    28681 tgcacataat ttgattgatg aagcaaagaa aagtatagaa aaattatctg atgatgtaga
    28741 ttttttaaag ggattggctg aatatataat agatagagaa tactaaaata tacagctaaa
    28801 atcgaggagg gaaaaatgga ttttttttcg gagattttta acaatggggc tttgggaata
    28861 agtctgattg cttgtttctt agcacaattt ataaaaatat ttactggtaa ggaaaaaaga
    28921 attgagttat caagaatact tatttcagga ggtatgccta gttcccatag ttcttttgtt
    28981 acaagtttag ctacagtggt aggtattgaa aaagggttta attctactga ttttgctata
    29041 attaccgtgc ttgccttaat tataatgtat gatgcagcag gtgttagacg ggctgttggt
    29101 aaacaagcta ctatattaaa tcaaatggtg gcagatatac agcatggaaa gcatatagag
    29161 cagaagaaat taaaagaatt aataggtcat acaccactag aagtttggtt tggagcttta
    29221 cttggaatag taacagcttt aatattgatg taaattgaaa atttacagaa taaaggtgag
    29281 gataatatgt ataaatattt agataaagta aattctccaa aggatataaa aaatatgagt
    29341 atagaagaaa tggatttact tgcaaaggat ataagaaagt tcttagtaaa atctgtttca
    29401 aagacaggag gacacttagc ttctaatcta ggagtggttg agttaacatt agcacttcat
    29461 aaagtatttg atagccctaa agataagatt gtatgggatg tagggcatca atcttatgtt
    29521 cataaaatag ttactggaag aaaagattgt tttgtatctt tgagacagtt taatggacta
    29581 agtggttttc caaaggagaa tgaaagtcct catgatattt ttgatacagg acatagcagt
    29641 acatctattt ctatagctac aggtattgcc tgtgctaggg atataaaaaa agaaaactat
    29701 agtgttatat ctgtgatagg agatggttct ataacaggag gtatggcact tgaagcttta
    29761 aatcaattag gatatataga taccaatatg atagtaatcc ttaatgataa tgagatgtcc
    29821 atagataaaa atgttggagg aatgtctaaa tatctgtcaa gtattataag aaactcaaca
    29881 gttgagaaaa tgacagatga agttgataaa attttaaatg ttacacaaac aggtgaaatt
    29941 ttatctaaga ctgcacatag atttaaagat aaactaatgt atagcttttc tcctcaggac
    30001 tgttcatttt ttgattcatt aggtataaga tattatggac ctatagatgg ccataatact
    30061 aaagaactaa tagatatatt gagaaaagct aaacacaaaa aagggcctgt tcttttacat
    30121 gtaattacga aaaaaggaaa agggtacaga tttgcagaag aacaacctga taaatatcat
    30181 ggagtatcaa agtttgatat aaagacaggt gttacatctg caaaagttaa gtctatgtca
    30241 attagtgttg gagaaaaact tgttgatatg gctaatagta atgaaaatat agtagcaata
    30301 acagctgcaa tgccatctgg tacagggtta aacctgtttg aaagtgcata tccaaaaaga
    30361 tattatgatg ttggtatagc tgaacaacat gcaacaggat ttgcagcagg tcttgcaaaa
    30421 aatggtatga aaccttactt tgctgtgtac tcatcatttt tgcaaagggc atatgaccaa
    30481 gttattcatg atgtatgcat aacaaaaaag ccagttactt ttcttataga tagagctgga
    30541 ttagttggaa atgatggaga aactcatcat ggtatgtttg atttaagtta cttaaattct
    30601 attccaaata ttgtagtaat ggctccaaaa gacacaagag agatggagct tatgatggat
    30661 ttgtcattaa aattagattg tccactagca attagatacc caagaggaag tagttattac
    30721 ctagataagg gagagtatgg agagattgta ctgggtaaat atgaggtatt agatgacggt
    30781 caagatacag tcatactttg tattggaagt atggtaaaac atgctttaga ggctaaagaa
    30841 atattatcaa gagaaggaat aaatccgaca atagttaatg ctagattctt aaagccaatt
    30901 gatgaaggta tgttaaaagc attattaaaa aatcataaaa atgtagttac tatagaggat
    30961 aatattgtaa ctggaggatt tggaagtaga ataaataagt ttattataga taatgaatat
    31021 aacgtaaata tacttaatat agcaatacca gaagaatttg taaaacatgg caatatagat
    31081 gaattatatg attttgtagg attatcgcca aaaagtattg ctgataaaat aaggaaatta
    31141 gttattgagt aaaaatctaa tgtagatatt gtaaattaaa atttatataa aatatagaat
    31201 ttatcaatta gaataaatat caagtgtgtt acttcaagct actttagtta tcattaaagt
    31261 agcttttaac agaaaggtaa ttgtatgaaa aaaagaatag atttattatt agtagaacaa
    31321 gggtactttg aaagtagaga aagagccaaa aaagcaataa tggctggatt ggtttttgtt
    31381 gataatcaaa ggtgtgataa ggctggtaca gaagtaaaag aagattgtag tatagaagta
    31441 aaaggaaatc caataccata tgtcagtaga ggtggtttaa agcttgaaaa agcaatgaaa
    31501 aactttgatt tgactattga tggaaaggta tgtatggata taggggcatc cacaggtggt
    31561 tttacagatt gtatgctcaa aaatggagca ataaaagtat tttctataga tgttggttat
    31621 ggtcaacttg catggaaatt gagacaagat gatagggttg tgtgtatgga aagaaccaat
    31681 ataagaaatg tgacaataga agatacaaag cagtttgctg attttgcgtc aattgatgtg
    31741 tcttttatat cgttaaagtt agtacttcca aaggcaaagg agttagtacg tcatgatggg
    31801 gaagttgtcg ctttaattaa acctcaattt gaagcaggaa gagaaaaagt aggtaaaaaa
    31861 ggagtagtta gagaaaaatc tacacatata gaagttataa aaatgatttc tgatttctca
    31921 gtcgaaaatg gatttgagat tttagggtta gatttttctc ctattaaagg tcctgaaggt
    31981 aatatagaat atctaattca tttgagaaat ggaaatgaag gatatgaatt tgatggtgag
    32041 acatacaata acaaaatagt agaagttgtt gaagcttctc ataatttaga caaataggtt
    32101 aaaagggggc ttagatttga tcctcgaatt atatatgaaa aattgtgcct tagttgaaga
    32161 actaaggttg aatattgata aaaacttaaa tatacttaca ggtgaaactg gttcaggaaa
    32221 atctataata atagatgcac ttggactttg tttaggtgat aaatatgata ggtctttttt
    32281 aagaaaagga acagataagg gacttgttga ggctgtattt ttttcagaca atagatatct
    32341 taaaaaaata ttagaagaaa atgatataag tatggaagat gataatttat tggtaataac
    32401 taggttgata tactcagatg gtaagagtac tgctagagta aatggaagga ctgttaaagt
    32461 gtctcttctt aaagaaattg catctacact tatagatata catggtcaac atcaaaatca
    32521 agctttgttt aataaagata ctcatttaaa atttctagac ctttttggag aaaatgaatt
    32581 ggaagagttt aaaattgctt ataaaaatgt gtaccataag tatagtgaag taaaaaaagc
    32641 tttaaattgc ttgacagaaa ataaagatga gatgcaaatt caacgagaaa tagatttatt
    32701 aagatttcaa ataaatgaaa ttgaagctgc aaatctaaat aagaatgaat atgaagattt
    32761 attaaaacaa agagaagttt atagaaatag tgaaaagata tataataatc taaattcgag
    32821 ttatagcaag ttacataatg gtgagtataa tgttattgac ctaataggtc ttgcatctaa
    32881 ggaacttaat gacatatcta aatatgacag tgttttaagt gaatatagtg atactgtaga
    32941 aagaatcatg tatgagttac aagacatatc tggtgagata agaaattaca aagataatat
    33001 agactttgaa ccttatgagc tagagcagat tgagcttaga atcgacgaga ttaataactt
    33061 aagaagaaaa tatggagaca gtatagatga tatatttgag tattatggaa aaattaaaga
    33121 tagattagat gaaatattaa acagagatga aagagttgaa cagttaagaa gcaaactgat
    33181 gaatatagaa gaagatttaa aaataaaagc cagtaaattg actaaagcaa gaatagaagt
    33241 agcaactact ttagaaaaaa tacttcttga tgaattaaaa agcttaaata tgaaaaatgt
    33301 aatgttcaag gttaattttg aaaaaggtcc atttacttta gatggagtag atgatattga
    33361 atttatgata tcttttaact taggagaaga tattaagcct atatataaag ttgcatcagg
    33421 tggtgagatg tccagattta tgttagcgtt taaaaccata ttggctgata tagatgatat
    33481 tgatacatta gtgtttgacg agatagatac tggaataagt gggatagcag cacagatagt
    33541 tggtgagaaa ttgagtgata tagcaaagaa aaaacagata atatgtataa ctcatttacc
    33601 tcaaattgct gcaaatgcag acactcatta ttgtatagag aaagatactt caaataatag
    33661 gacgtttacc aatgttagta agttaaatga atctcaacga aaaaatgaaa ttgccagact
    33721 tatagcaggt aacaatataa cagaaaaaac tattgaacat gcaagtgaaa ttattgagtt
    33781 ggctaaaaaa tgttagtgaa aaacaagcat tttaataggt gtaaagaatt tttatagaaa
    33841 tgctttacat ctatttttta tgatgaaatt attttaaaat atctagtgcc tatattgaag
    33901 aaaagtagat ttaaaaatag aagaaaattt gtataaattt gtaactttga aatttctaaa
    33961 tgcttaagta taatattatt tattaaggta gtagtgaggg ggacataaaa tgaataaaaa
    34021 agatactatg cacttaattt ttacaattgt aatttttatt ttattatggt atattgtggt
    34081 agcagtaagt gagattaata aaaatattga aagttgtagt aataaattag attatttgtc
    34141 acaagagatt atgagtacta aatcggatat tagtaatact ataaatgaag aaatgtcaaa
    34201 aagttatata acaaagagta ttgacttaaa agtaaaaaaa atcgagaaaa aagagtgtgt
    34261 tattgatgta gatgttcaat taagtaaact tggaaaaaat ggaaaagtat tttttatgta
    34321 taaagaagat agtgataaat ggaatgaaac tgagatgaca aaacatggag aattatcata
    34381 tgcatgtgaa attaagataa atacaggaag tgaatatgac tataaagtag ttacaagtgg
    34441 agctatatct gaaagttctg atgtgcaaaa attgactaaa tctgattata tgcctgaaca
    34501 acccatattt aattctggga taagagataa tagagaatac tttattgaaa tagtagaaaa
    34561 tagtaatctt ttcaatcatg agagtgaaaa gtcaaaaaat aaagtttatg ataatattag
    34621 attagaaaaa gtagatatta ttgtaaatga aggaaaaaaa gacactatat acaaagccaa
    34681 attagctgaa ggcttagatg atggcaaaac taataattca aatagaaatc aagtgaatta
    34741 tgaagtaagt tttcctaaaa gggatattga tgacataatt tatattaaag ctaaacttac
    34801 atacaataat ggagtagaat atacaaagga tataactgaa gatataacta tgggattaca
    34861 agatttagaa aaataaatac atatttaaat aaatattcaa atagatttgt tcaataaaat
    34921 agagtataaa taaaactgta gtagttttac ttatactcta ttaaaaaatt ataattttaa
    34981 ttccatatat ctagcccctt caattggatt ataagtataa ggttctatat cataaaaacc
    35041 aaacttttca tataatcctt gtgcactttt catgcttgga agagtatcta atctcatgtg
    35101 tgtatatcca aattttttag cttcttcaat gatttcttcc aatagtattt ttcctatttt
    35161 taatcctcta aattgatttc ttacatataa tctttttaat tcacatattt taccttcaag
    35221 tttttttaaa gcaacacaac ctgccaaatt ttcatcaaca aatgccaata tcaatgagcc
    35281 tgaaggtttt ttatattttc ctggaagtgt ttttaattca ttattaaagt cttgaaaaca
    35341 taagtctata tttagagaat ttgaatattc agtaaataga agttttacgt tttcaaggtt
    35401 ttctaaatct ttttcttcaa caaattgtaa agttatattt tgagccattt tatccgccat
    35461 tatataaaca tgtttatata atgacttcac ctccttgata tatgtatatt tttcttaata
    35521 tcaattagat tttaatacca ttactttttc accaccatag tcaagctctc cattaaactc
    35581 aaatccaaaa tctttataaa gcttaatagc cattttattg tcttcgaata tactaagata
    35641 tagttcatca cagttatatt catttttaag atgatttaca agaaaatcta atgatgcttt
    35701 tccatatcct ttaccttgat attttgaatc aatcataaat ctatcaagcc aaactctatc
    35761 atcttctaaa cgaccataca tagcaaatcc tattaaagaa ttttcatcat atattccaac
    35821 aggtacccaa gctgttataa atttagattc agcaatagaa agtgcattgc tttctatata
    35881 atctaactga cttttatcta cagacaaact aactaaatca tgccaattat ttatgtctgc
    35941 aattcttaca tttaatttca agatattacc gcccttcaaa ctttttgtta caatctaata
    36001 ctatcatatt atgtattaaa aatcacttat taagatttta aataaacttt ttaaatgaat
    36061 aaattctcag gaaaaaggaa ttataaatac ataataatac agcataggag tagatggata
    36121 tatgaacaaa ataaataaaa atttcaaaaa tatttggata atattaggag ctgtatcgat
    36181 ttttaccatg gttataattt ttaataaaaa ttgtaatgtt aataaagaga ctcaaataga
    36241 agcttttaca attaagaatc atgataaaaa atttgtatat cctatgggaa atataattgg
    36301 agtaaaagca aatacagatg gtgtattggt cttaggatat gaggaagagg atgtagacta
    36361 tattggaggc atacaaatcg gtgataatat agtaaaaata gataataaaa ggataaaaaa
    36421 tagtcaagat gtatcagaga ttttgaataa gattaaaaaa agtaaagttg aagtgacttt
    36481 tgagagaaaa aataagtata aaacagaagt aatagagact aaaaaagaaa atggcaagta
    36541 taaacttgga ttatgggtaa gagataaaat atctggaatt ggaactatga ctttttacga
    36601 ccctagtatg gaaaagttca aagcaatcgg tcatgccata aaagattcgg atactaatga
    36661 actattaaag ataaagcaag gatatatata taagccagaa caattaaaaa tagtaaaagc
    36721 tagtaatgaa aaagtaggta aaataaaagg agattttaat gacagcaatt taatgggtaa
    36781 tttctcaaat aattcagagt taggtataag tggtaatatt acagaaaacc ataataaaga
    36841 gtttaatgta gcaaataaag aaaagcaatt aatagaagtt ggaagaccgc aagatgtaaa
    36901 aataggagat gcagtaattc tttttgagga taaaaacaaa aatataacaa gctatgatat
    36961 aaaaatagaa agtatagtat atgataaagg aaattataga gatatggtaa taaaagtagt
    37021 agatgacaag ttattggaat acacaggagg tatcgtacag gggatgagtg gagctccaat
    37081 aatacaaaat aataaaatta ttggtgcaat aactcatgtt tttagagata atccgaaaaa
    37141 aggatatggt atttttatag atgaaatgat aaaattgtag gtgaggcatt aaaaaatttt
    37201 attattttat caattatcta ggaggaatat aattttggag tgtcgaatat gctttagagt
    37261 agataattag gaagcaattg tgtaaaaagt ttagttttct gtaataagaa gatgtttttt
    37321 aatgggggga tttttagtgg aaaaaatcaa aatagtttta gcagatgaca ataaggattt
    37381 ttgtcaggta ttaaaagagt atttgtctaa tgaagatgat atcgatatat taggcatagc
    37441 taaggatgga attgaagcat tagacttagt aaaaaagaca caaccggact tattaatact
    37501 agatgtaata atgccacatc tagatggatt aggtgtaatt gaaaaattga atactatgga
    37561 tatacctaaa atgccaaaaa taatagtact atcagcagta ggtcaagata agataactca
    37621 aagcgcaata aatctaggag cagattacta catagtaaag ccatttgatt ttgttgtgtt
    37681 tataaacaga attagagagc tagtttctaa tagagttaca caagttgagc caaaacctag
    37741 accagttcaa gagactcaaa tgactagaag tgattttgtt aagaatgtag gaaatataga
    37801 gacagaaatc acaaatataa tacatgaaat aggagtacca gctcatataa aaggatattt
    37861 atatttaaga gaagcaataa aaatggttat tgataatgtt gagcttttag gtgcagtaac
    37921 aaaagagtta tatccaagta tagctaaaaa attcaataca acaccaagta gagttgaaag
    37981 agcaataaga catgcaatag aggttgcatg gagtagagga aaagttgaca caataaatca
    38041 attatttgga tatacggtac acaatactaa aggaaaacca actaattcag aatttatagc
    38101 aatgattgct gataaattaa gattagaaca tagtatggtt aaataaacaa gacataaaaa
    38161 gtaaggcttt ttaattaagg cattggctat aaatgcgtat tacaagcagc gaaacggtat
    38221 aaccactagg gttataccgt ttcgctattt taataaatat aaaaattttc tttattattt
    38281 gcttactata tcaatataat aattttatta tactatggat atagtatgtg tctttacaag
    38341 ttgtaaactg acagtggttt attttttaat ataaatattg actttgatgc aggtaaactt
    38401 tgtattttta agcgtattat ggaatatgtt aaataaaaaa atgatgaaat atagtattgt
    38461 aaatgccaaa gatgcaaaac aaacttaaaa catttatttt attgttaagt aatgctataa
    38521 tataatgtga ttttaataat gatagtggag gtttaaatat gagagtcgag gcccctataa
    38581 aagtagatcg aaaaaccaaa aaacttgcta aaagagttga aagtggggaa atagcagtta
    38641 taaatcatat agacatagat gaagttgctg caaactcttt agtagaagct aaaataaaac
    38701 ttgtcataaa tgcggctcct tctataagtg gtaggtatcc caataaaggt ccagggatat
    38761 taactgaaaa taatatacta ataattgata atgttggaga agagttattt gaagaattaa
    38821 aagaaggaga aactatagaa gttgtagatg gaaagatata tagaaaagga aaatttttag
    38881 gtgcaggaga agttttagac aagtatgagg tttcacacca aataaaggct gcctatgaga
    38941 atttagctgt agaattggat agatttatag ataatactat agattatgct aaaaaagaaa
    39001 aaggatttat acttggagaa gtagaaattc caaaagtgaa aactaattat gctaataaac
    39061 atgtattgat agttgtaaga ggtcaggact acaaagaaga tttaagtact atgctttctt
    39121 atatagaaga ggtaaagcca gtattggttg gtgttgatgg aggagcagac gctttaattg
    39181 agtttggata tactccagat gtgattgtag gtgatatgga tagtgtaagt gatgaagctt
    39241 taaaaaaggc tagtgaaata gtagtacatg cttatacaga tggaagagca ccaggtctta
    39301 agagggttga ggaattaggc ttagatgcag ttgtatttcc tgcgccagga actagtgaag
    39361 atatagctat gcttatagcc tatgagtata aggctgagtt aatagtagca gttggtacac
    39421 attcaaatat gattgatttt cttgaaaaag gaagaaaagg tatggcaagt acttttttag
    39481 ttagactaaa aataggttca aagcttatag atgctaaagg cgttaatttg ttatacagaa
    39541 gtaaattaaa aataaagtac atatgggcat taatagcaac agcattattt cctgtgttgg
    39601 tagttgcatc attgtcacca ggagttcaac agttcataca actaatgcaa ttaaagttta
    39661 gagtattatt acagatgtaa attttaggag gataaatatg catattaata tgaaatatta
    39721 tatagtcact ataggggcta tatttatagc tttgggtata ggcatgttag taggatttaa
    39781 tttaaataat aatcaacaat taagtgaaca acaagcaaat ataataaatg atttggatga
    39841 taagtttaat atcttaaaag aaaaaaatga taaacttgac agtgacttag caagtgtaaa
    39901 tagagattat gaagaggcag ttaattttat aaataaaaat gtagataaga ttttagctgg
    39961 ttcgctgaat ggtaagagta taggaataat atctgctaat gaaaatgacg attatacaaa
    40021 aaatatagaa gacataataa ataaatctaa tggaagcatt gcatttaata taatattaaa
    40081 agaaaacata acaaatccag aaaagttaaa ggaaatgtca actaaactag gtacagatgt
    40141 aaaaaatgca aatgatgcag ttaattatat aattgacact cttaaaaagg aagatgcaag
    40201 tgacatttta acatatcttc aagaactaga tgtaataaag tttaatttta ttggagatac
    40261 atatctaaaa tatgattccg ttgtaatagc tggtggaaat gatgcaaaag acagtacaaa
    40321 acagtttgaa aaaatagaaa aatttgtagt ttcaaaactt aaatcagaga ataaatattt
    40381 agtagaagtt caaaatacag gagttaaaac ttcttatgta gaactatatt ctaaaaacaa
    40441 agttgcaact atagacaata ttgatgaagg tataggaagt atatctctag cgattcttct
    40501 acaacaaggt aatatagttg gtaattttgg tagattggat actgcaactt cattgttgcc
    40561 atctattaaa tagaaataat tttaaggaga gaaaaaatgg attcttatat aagtataata
    40621 ataccagctt ataatgaaga aagtagaata aaatatacat tagaaagcat attggacatt
    40681 aagcaaataa atgaaataat agttgtggat gatggttcaa ttgacaatac tgctaaggtt
    40741 gtatctgagc tagaaaatga aaagattaaa ttttttaagt tagataaaaa tagaggtaaa
    40801 ggatatgctt taaattatgg tatcaagtta gcaatgaaaa atgcagatat tataggtttt
    40861 ctggatggag atttaggaag ttctgctaaa gaagttgaaa aattaatagt acctatatta
    40921 aatggtgagg cggatgttac aatagccaag tttcctcctg caaagagaaa aggaggtttg
    40981 ggatttgtaa aaggtttagc taaaaattct gtgtttgaga tgactggtgt tgagttagat
    41041 gcaactttat caggtcaaag aatatttaaa agagaagttt tagaaaagtt tgatgaaata
    41101 ccttttggat atggagtaga agttggaatg accattgata tattaaagta tggatttaaa
    41161 attaaagaag tattagtaaa tatgactcat aatgaaacag gaagaaattt aaaaggtttt
    41221 attcatagag ggaaacagta ttatcacata aaaaaggttt taggacaaaa aaagaaagaa
    41281 tggaggtaat atcttagtga aagattatat actttatacc attttattat taactggatt
    41341 atttggaacc tatgctgtaa ttccactatt taaaaattta ttgataaatg gaaatgtact
    41401 tagaccaaac tataaaaaag atatgatacc agtaagtatg ggaattgtat ttttgcctat
    41461 gattgtaatc aatggaataa tacttgcttt ctttactaca gaatttaaga atttagcgta
    41521 tatatttatg tttatgtttg gtatgatatc catgtttttt gcagggatac tagatgatgt
    41581 cattggaaat agagatgtaa gtggtttaaa ggggcatttt aaaagtttat ttaaaggaag
    41641 tcttacaaca ggaggtttta aagctttatt tggtggattt gtagggctta tagtttctgt
    41701 tgctatatca aaagacataa ttgatataat agtaaataca ctaataatag ctctatcaac
    41761 taatttaatg aatcttttgg atttaagacc aggaagggca ataaaagcat atttattcat
    41821 aatggtaatt gtatttttaa cattgacagg atttgttcaa gttcttccat tacttatagt
    41881 accaaatgta cttgcatact ttaattatga tttaaaagca agggctatga tgggagatac
    41941 tggttcaaac gtccttggaa tatctattgg aatgctcatt tcttttgggt atccatttaa
    42001 cattagatta ggatggttaa tatttgtagt tattatgcat atattcactg agaaatactc
    42061 attaactaag ataatagaaa aaaataaact attaaatttt atagataaat tgggaaggta
    42121 gtattatgat aagcaaaaga gtggggattg ttgaatcaat agtaagtcaa actgaaactc
    42181 tagatgatat aagagtaaat ataaatggag aaatacaaaa ggcatataat tatccaaaaa
    42241 tatctggaac tataaatata ggtgacgaag tggttttaaa tacaacagca gtagaactga
    42301 gcttaggtac aggtggatac cattttgtaa taactaattt aaataatata gaatctactt
    42361 taactgaagg tggacatata atgaaactta gatatacgcc tcttcaaata aaagtggatt
    42421 ctgttgagga acaagaaagt atttatcatg ataaatttgc gaattttaaa ggattagaag
    42481 gtctttctgt tgtagtaggg actcttcata gtatgttaac tccatttgca gcttcattta
    42541 aaagaaataa tccaaataaa aaattagtgt atataatgac tgatggagca tctcttccaa
    42601 tttatttaag taaaaatgtg gatactttaa aagaaaaaaa attaatagat agtacaataa
    42661 ctataggaaa tgcgtttggt ggagattatg aatgtataaa tatatatact gcattaatta
    42721 cagcaaaaga aatactaaaa gcggatgcag tatttgtcag tatgggacct ggaattgctg
    42781 ggacaggaac aaaatatgga tttacaggga ttgaacaagg ctctattctt gatgctgtaa
    42841 aaaaacttga aggaagagca attgcaattc caagaattag ttttgctgac aaacgagaaa
    42901 gacatcaagg tatttcacat catagtatga ctgtatttaa agaaattgta aatgtaaatg
    42961 tagacatacc aataactata tatgatgatg aaaaattgaa ctttataaaa gagcaaatca
    43021 atttaaatgg tttagacaaa aagcataata taatttatat agataataat aaaacgaaag
    43081 aggatttaga ctattttaat ctaaaagtaa agagtatggg aagaaattat gaacaagatg
    43141 aggcattttt caaagctgca agcacagcag catattactt aatggaggtt taagatggta
    43201 ttagaagaaa aaactattag tagtgatagg gtttatacag gtaaagtaat tactttaaag
    43261 gttgatactg ttgaaattcc agggcaagga tatcaaaaaa gggagttagt agaagttggt
    43321 ggagcagtag gaatagttgc aatcacagat gataataaag ttgtgttagt taagcaattt
    43381 agaaaaccta tagaaaagcc aatctttgaa attccagctg gtaaactaga aaaaaatgaa
    43441 agtccaaaag aatgtgctga aagagaatta aaagaagaaa ctgggtatag tgcaaaaaat
    43501 attaagctaa tacataaatt tttcacttca gcaggttttt ctaatgagat aatgtttgta
    43561 tatttagcaa caggtcttac tccaggagag aataatttag atgcagatga gtttttagat
    43621 gtttatgaaa ttgagcttga agaagcatat aacatggtat taaaaaatga cgttgaagat
    43681 gcaaaaacat ctattggatt attattagta aaagatatgt ttaaaaatta atatttataa
    43741 aatgaataac cctttgtcct aaagcatatt attttataaa ggacaagggg gaattaaatt
    43801 gagaagaaca tatagaaaaa atgattttag agaattaaat agacaaataa ttattatagg
    43861 attgttgttt atgatgtcta ttgttgttgg gtcttacata aataaaatat tgccaggttc
    43921 tagtaacaat atattaaaca atataaatcc tgctgtagag tattataatc taaatataag
    43981 cataaaagat acagtgatac aaaatttaaa atcagatgca atctttatgg gctcaatagc
    44041 tctattaagt ctatttgtag taacaattcc agcagtgttg gtagcttttg tattaaaagg
    44101 gatgtcaatt ggttatacaa taaatagttg tatattggca ttaaaattta aaagtataaa
    44161 aatgatttta ataatattat ttaaaaattt gattattatt ccatgtgcaa tcatattagc
    44221 attaattagt ttaagttatt ttaaagaaat ggtttatgaa tttaaaaaga aaagtagaaa
    44281 aaatatgcaa tttttaatta agaggtatat actaaatatt ataattataa tagcactatc
    44341 attggggttg caattaatat taaatactgc aagtattggt attattaagt tcctagctaa
    44401 ataaaggagt ttataatgga tattatagag ggatatatag attacataaa agataaaaaa
    44461 aaattatcag agaatacagt ggcatcatac tttatggata taaaaaaata tatggaatat
    44521 ataaatcaaa aaagcattaa actagtagat atagtagaaa atgatattat aagttactta
    44581 ataaagttgg aaaaagataa tgtatcaata gctacaatag cgaggatgat atcctctata
    44641 aaatcttttc atgattattt atttttaaat catatatgta caaataatcc tgcaaaggat
    44701 ataaaaaaac ctaaaataaa aaaagaaaat ataaatatac ttacagaaga agaaattgaa
    44761 agactattaa attttccaaa gttaacaaca cctaagttga ttagggacaa ggctattttt
    44821 gaagtattat atggtactgg tataaaagta tctgaattag tagagatgaa tatagaggat
    44881 attgacctag atattgatta tatatattgt aattcatgtt gtaaaaatca aagagtgata
    44941 ccactttgtg atattacaaa actttactta gaaaagtatc taaaagaagc aagacctaaa
    45001 atggctgcag aaggtgaaaa atcattattc gtaagttcat taggtcaaag gtttacacga
    45061 caaggtcttt ggaaagtcat aaaaaaatat tctaatctag ccaatataga taaaaatata
    45121 aatcctacaa tgcttagaca ttcatttgct attcatttgt taaatgaagg tgctaacatt
    45181 gctgttgtaa gtaaaatttt aggaaatgtt aatttatcta gtttgcaagt atatttaaat
    45241 catatagata aaaacgtgag aagagaaata aaggagaaac atcctagaaa tgatgttgat
    45301 gtagaattac aaaaagcaga agctaaatag actatatagc aaattttata atttagaagg
    45361 agcatattta tatgagcaga gtaatttgga ttgtaattga tagtgttggg ataggagcat
    45421 tacctgatgc tgaaaaattt ggtgatagta aggatgtaag tacccttgga aatatattta
    45481 aggaatatcc agatattcag attcctaata tgagaaattt aggtatagga aacatagaag
    45541 gtatagattt ttttgaaagt ataaaagaac caataggttg ttttggaaag tgtaaggaaa
    45601 tgtctcaggg aaaagatact actacaggtc attgggaaat gactggtata atagttgaca
    45661 aaccatttaa gacatttgaa catggattct ctaaggagat aatagaagag tttgagaaaa
    45721 aaactggaag aaaagtagta ggaaataaac cagcttcagg aactgtaata atagatgagt
    45781 atggagagca tcaaataaaa actggagatg taatagtgta tacatcagct gatagtgtat
    45841 ttcagattgc tgctaatgaa gaagtaattc ccctagaaga actctataat atgtgcaaaa
    45901 tcgctaggga aataatgatg ggggataatg cagtagctag agtaattgct agaccattca
    45961 taggtaagaa aaaaggtgag tttgtgagga cttcaaatag aagagattat tcactagacc
    46021 catttgaacc aacagttctt gataatataa aagaatcagg acttgatgtt ttagcagttg
    46081 gaaaaataga agatattttt aatggaaaag gaattacaga tgctatccac actaaaagca
    46141 atatggatgg agtggatgaa actttaaatt atatgaaaca agataataaa ggtctaatat
    46201 attcaaatct cgttgatttt gattctaaat atggtcatag gagagaccca gaaggatata
    46261 agaaagctct tgaagagttt gatagtagac ttcctgaaat aatggctaat atgagagaag
    46321 atgatatttt aataatcaat gcagaccatg gaaatgaccc aacatataaa ggtacagacc
    46381 atacaagaga atatatacct gttatgattt atggcaataa gattaaaaaa ggttttaatt
    46441 taggagtaaa agatactttt gctgatattg gagcaactgt tgcagatata ctaaatgtaa
    46501 agttgcctaa acatgggtca agctttaaag aagatttatt ttaaactata aaatgataaa
    46561 aacttagctt aataggatgg agagattgga atgtttgaaa agatagcaca aagtagtaaa
    46621 tttataaatt ctaaaagtaa tataaaacct aagattggtt tgatattagg ttctggacta
    46681 ggtgatttag caaatgatat agaagaccct gtcacaatta aatatagtga aattccaaac
    46741 ttccctgtat caacagtagc aggacatgct ggacaacttg taattggaaa acttgaagga
    46801 aaagaagtta ttgctatgca aggtagattt cattactatg aaggatattc tcagaaagaa
    46861 gctacttttc cagtaagggt tatgaaagaa ttaggagtcg agattcttat agtaactaac
    46921 gctgctggag gcgtaaataa agaatttaaa gcaggagatt tgatgcttat aagagaccat
    46981 ataaacttta gtggaagtaa tccattagta ggaaaaaatg atgacagatt tggtgctaga
    47041 tttccagata tgtcagatgc atattcttct aaatatgtag atgtagttaa aaactgcgct
    47101 aaagaatgca atatagatgt caaagaaggc gtttatatgt tttttagtgg tccaaactat
    47161 gaaacaccag cagaagtgag aatggcacaa attttgggag ctgatgctgt aggtatgtct
    47221 actgttccag aagtcatagt agcttctcac tctaatatag gagttattgg tatatcttgt
    47281 ataacaaata tggctgctgg catcttaaat caaccactaa gtcatgagga agtaatagaa
    47341 actactcaaa aagttaaatc agaatttatg aatttagtta aaacaacggt taaaaattta
    47401 taagttaaag cttattatag attttttgag gaagtttata ataaaatagt taatgtttaa
    47461 tttgaaaaga attaataaaa agctgtcttg atatagaaag ttatttctaa taaaggacag
    47521 tttttttata atactacaac atttgaaagt gttttaaagt ttaatattta tttaaattat
    47581 tataggttag aatggagatt tcaattatga gaatgtatga tattataaaa aaaaagagag
    47641 ataacctaga gcttagtaaa aaagagataa actactttat agaaaactat tcaaagggag
    47701 atatacctga ctatcaagct tctgcactat taatgtcgat ttatttaaat aagatgaata
    47761 aacaggaaac tgtacatctt acagaagcaa tgatgaactc tggagacatg ataaacctat
    47821 ctgagataga tggaataaag gtagataagc atagtactgg tggtgttgga gataagacta
    47881 ccatagctct tatacctttg gttgcttcat gtggagcacc agttgcaaag atgtcaggta
    47941 gaggtcttgg tcatactggt ggtactattg ataaattgga gtctatacca ggtttttcga
    48001 cagaaatgga gataagtaaa tttattgatt ctgtaaataa gtctaaaata gcagtttgtg
    48061 gtcaaagtgc aaaggtagca attgcagata agaaaatata tgctcttaga gatgttacag
    48121 caactgttga taatatatca ttgatatcat caagtgtaat gtctaaaaag ttagcttcag
    48181 gagctgatgc aattgtgcta gatgtaaaaa ctggtgatgg agcatttatg aagacattag
    48241 aagagtcttt tgagttagct aaatctatgg ttgatatagg tactggtatg aatagagata
    48301 caatagggat tgtaacagat atggatgaac ctttaggttt tgcagtggga aactctttag
    48361 aagttataga ggcaattgag acattaaaag gaaatggacc taaagacctt gttgagctat
    48421 gcgaagtatt aggagcatat atgttggttt tatcaaaggt agcacatgat tttgaagatg
    48481 gtgttgaaaa aataagagaa tcaataaaat ctggtactgc tatacaaaaa ctcaaagagt
    48541 ttatagagaa tcaaggtggt aataaagatg tagtagatga ttattcttta ttcaaaaaat
    48601 caaagtataa atatccagtt aaatcaacta aatcaggtta tataagcaaa attaaagctg
    48661 aagatgttgg tgtatcggct atgattcttg gagcaggtag agaaactaaa gatgatattt
    48721 tagatttatc agcagggata attttagaaa agaaagtagg agattatgta aatgaaggtg
    48781 aagttttagc ttacatgtat tataatgatg aacaaaagct ttcattggca aagggaagat
    48841 ttattgattc ctactctatt gtagattcaa aaatagaaaa aaataaatta atatatggta
    48901 ttgtaactaa agaagagatt aaaaagtttt agaaaatttt agaaaagtga ttataaaagg
    48961 cctaacatgt caaaaataat gttaggtttt ttattttttg ataaactata aattatacca
    49021 aaaatatata ttttaaaaca ttttttacaa tataaataag tttattctac aagatgattg
    49081 aaaattaaag tatctagttt tataatagaa aatggataaa tatacataag gagtagatta
    49141 tattatgaac tttaaagaaa atagattgaa aatagcagta ttaataattg ttattttgat
    49201 tatcttagca ggtatatttg tgtttataca aataggtcct tatgataaaa ataacaaaaa
    49261 agatgtaatt atcgacgttc caagtggagc atctgttgga aaaatatctg acatattata
    49321 tgaaaataaa ttaataaaga atgaactatt atttaaactt ttggtaaagg ttagcaataa
    49381 agctccgtct ataaaatcag gaacatactt acttaatcaa tcgtattcaa acaatgatat
    49441 aataagtctt ttagtttcag gtaaaatata tcaggatggt ataaaagtga ctattccaga
    49501 gggagctaca tctaaagaaa ttatagcaat gttagtaagt aaaaatttag gagataaagc
    49561 tacttttgaa aatttaataa aaaaacctca agaattttat gataaatttt catatttgaa
    49621 ggaagatgga ataacctctt tagaagggtt cttgtatcca gaaacttatt attttaattc
    49681 aaagaaacaa tcagaagaag atattttaag cgaaatgtta aaagtatttg acagtaaata
    49741 cacggacaag tttaaaaaga aacaaaaaga attaaatatg actcttcaag aagtaatgga
    49801 aatggcatca ataattgaga aagaagctgt tcttgacaaa gacagaccta taatagcaag
    49861 tgtgttttat aatagactta aggtaggtat gcctcttcaa tcagatgcta caatacaata
    49921 tatatttgaa gagagaaaga aaattgtaac atatgatgat ttaaaaattg attcaccata
    49981 taatagttat aaaaataagg gattacctcc tacaccaata tcaaatcctg gtataaaatc
    50041 tattgaagca gcattatatc cagagaaaac agactattta tattttgtag ctaagattga
    50101 tggtggaaat aattatagta caaactatca agaccattta aaatacgtta aagaatataa
    50161 agaagcaagg gataaacaat caaaagatac aaaagcaaca aataaagaaa atacaaaaaa
    50221 ataaaccatg attataaaaa gtctaacagg gtaagaataa tgttgggctt ttttattgaa
    50281 aattgttatt cttagattta taataaagaa agtgtaaata tatataacaa tatgaattta
    50341 aaaaataata aattaatata aaacttggat atttattatt ttagggagtg tttttatgag
    50401 taatatagtt aatgatttgg ttgaagatta tataagagtg acattaaaag aaaaagaagg
    50461 atttttaaaa gatttagaaa tttatgcaga ggaaaatagt gttcctataa tacataaaga
    50521 agtttcagat ttattaaaag tcttgttaaa ggttcaaaaa ccaaagagaa ttttagaagt
    50581 aggatgtgca attggttatt cttctatttt ttttgcaact attataggaa aagatgcaga
    50641 tataataact gttgagagaa atgaaaaaat gatagaaaaa gcaaaagaaa acataaaatt
    50701 agctggattt gacaataaca taactatact agaaggagat gcagaagaaa aacttagtca
    50761 agtccaaggt gaatttgacc ttatatttat agatgcagcc aaaggtcaat ataagctatt
    50821 ttttgattta gttatagata aattaaaaga tgggggactt ttagtatctg acaatatttt
    50881 gtataaaggt atggttgctc atgatgattt tgttgtaaga agaaaaaaga ctatagttaa
    50941 aagaatgaga aagtatttag attatatatg taattgtgac tatttaagca catcattggt
    51001 accaataggt gatggtgtag cactaagtta taaagaaagt aaaagaggtg atgtagatgg
    51061 aattaaataa agtagaactt ttagctccag caggtgattt ggagagattg aaaatagcta
    51121 taacatatgg agcagatgcc gtttatatag gtggagaaat ttttggaatg aggtctgcag
    51181 cgaagaattt tagtaaggaa gatatggcag agggagtagc ttttgcacat gaaagaggca
    51241 agaaagtatt cgtaacagta aatataatac ctcataatga agattttcta caattagaag
    51301 actatttgtt ggaactagaa gagataggaa tagatgcagt tataatagct gacccaggag
    51361 tgcttagtgt cattaaaaaa gtaattccga atatggaaat acatttaagt acacaagcaa
    51421 atacaaccaa ttatctaagt gcaaacttct ggtacgagca tggaataaaa agagttgtag
    51481 tggcaagaga attatctttt gatgaaatat atgagataag agcaaaaaca cctttagata
    51541 tggatataga agcgtttatg catggtgcta tgtgtatttc ttattctggc agatgtttaa
    51601 taagcaacta tatgactgga agagatgcaa acaaaggttc ttgtgctcaa tcttgtagat
    51661 ggcaatatca tttggtggaa gaaaaaagac ctggtgagta ttttccaata tacgaagatg
    51721 aaaggggtac attcttcttt aactcaaaag atttatgtat gatagagtat ataccagagt
    51781 taatcaaatc tggtataaca agtttaaaaa tagaaggtag aatgaagact gcatactatg
    51841 tagctactgt tgtaagagca tacagaatgg caatagatga attttacaga gacccagaaa
    51901 actggaagtt taatccaatg tggatggaag aactaaaaaa aggtagtcat agacatttta
    51961 catcaggttt ttacttgaat aaaccaacaa cagaagacca aaattatcaa tcagcatcat
    52021 atgttagaaa ctatgatttt ataggtatag ttagagaaac agaagatgaa gatggtctta
    52081 ttgttgttga gcaaagaaat aaaatgtgtg ttggtgatga aatagaagtt atgggaccat
    52141 ataaagaaac tatgtttact aaaattgaag aaatgtataa tgaagaagga gaagctatag
    52201 aatcagctcc tcatcctaga caaatagtga agttaaagct atcagtaaaa gttggtaaag
    52261 attatatgct tagaaaagtt atagaagaaa aggttgaaga ataataaaaa attgataaaa
    52321 atagtattag ccccttagaa attgtcaata atgaaattat ctaaggggtg atttcatgtc
    52381 aaaaaagaag actccatttt tgaaaaaagt gggaaagaga agctggtgta tatttactat
    52441 aattttaata atttatagtg ttttaattta taggctagtt gatatacaag tattaaaagg
    52501 tgataaatac aaacaaagtg ttgaatcaca aagcgttgag aaagtcgagt taaatagtgg
    52561 aagaggaata atctatgata gaaacaacaa gaagcttaca gatacaagta aatctcaggt
    52621 attaattgtt gaaaaagaaa aattaaataa taactataaa attttggagc ttattaagaa
    52681 agctactaag atgaatgatt tagatatata taaagctgta caagagcaat tgacacgccc
    52741 tataatacag attcagacta aaaatatcga tatcgataag agtatgaaaa aagaacttga
    52801 gaaaaatgga ataatggtag aagaaaaaac tatgaggtac gcaaaagatg gtttattatc
    52861 acatacaata ggatatataa aagaagatga taaatcaggt caatcaggaa ttgaaaagag
    52921 tatggatagt gtacttagaa attcaaatga aaagtatata agtgctttta aagctggaga
    52981 tgcaggaaat gaaaaatctc taaacatact aaagggaagt gtaaaaactg ttgataataa
    53041 ggataaagat agacatctaa aaactactat tgattataac attcaaaaaa aattagaaca
    53101 aatactaaat aaagaagaaa atcctacagc agcaataatc tcggaagcat ctacaggtga
    53161 aattcttgca atgtgctcta gacctaattt tgaccaaaat gacatatcaa aaagtttaaa
    53221 aggaaaaaat ggagaatttg aaaatagagt tataaaagcg acatatccac cagggtctgt
    53281 atttaagatg gtagttttat tttctgcact tgaaaatggt gttatagatg agaactatac
    53341 ttacaactgt acaggtaaga ctaaagttgg gaatactaat gaaatactgc gatgtaataa
    53401 aagagatggt catggtttcc aaaacttaag acaagctttt tctaattcat gcaatcctgc
    53461 atttttagat atagctatga aacttggaaa agaaaagata ctcaaatctg ctgaaaaatt
    53521 acatttattt gaaaaggttg atattggtct tgatgaagaa aaaattagag aagctccaaa
    53581 aaacatatca ataagaaatt tagctatagg tcaagaaaat atagaattta caccacttca
    53641 aataaatcaa atgactcaaa taatagctaa taatggaaca ttcaaaccac tatacttata
    53701 taaaagtctt gtggacaata atatgaatac aataaaaact tataagtctt caaaaaaaga
    53761 agaacttatt tctccttatg tgtgtacaca agttaaagaa tatatgaagt ctgtatctag
    53821 gactggtaca gctaaagacc taaaagatat tgaaggtggt tgtggagtta aaactggtac
    53881 agctcaaagt agtttaaata aaaaagctat agaccacgga tggataacag gattttatcc
    53941 tgaagaaaga cctaagtatg taataactgt gttagtagaa ggaactcaaa aaggaaataa
    54001 atctgcaaca cctatattta aggaaatatg tgaaagtata aaataaatta tgtactataa
    54061 aatttaagga tatatcgtaa ctttgatttt agatagaaaa ttttgtctaa tgatgtacga
    54121 tatatccttt taaaatatag tattaacttt cttgaacaag ctctttttct agttttttaa
    54181 gagctttttt ttcgattcta gatacatatg accttgatat atcgagtttt tgtgcaattt
    54241 ctctttgtgt cttatagcca taaggagtta acccatatct taactggaca atttctcttt
    54301 ctctaggagt aagaattttg tttagttgtt cgtaaagttt tccaacttga acttttagtt
    54361 caacttcatc aagaacatag ttagcctctg taccaagtat atctaaaaga cttatttcat
    54421 tacacactac tctttatatg ttattgatat tactagtctt catgtaattt caaatgaacc
    54481 attaattcaa aagaatctag tttttgttct ttagacttat aataaactgc tttatcaatt
    54541 acagatttta ggagtttatt ttttccaagt gaatctgaat catggtaaag tgataacaca
    54601 gttttaatat cttctattat tttagcattg tcttttttaa cagtactcaa tctatttttt
    54661 actgtcttaa ttgaatcttt aatttcttca gttcttgaac ttatattgtt ggacctttca
    54721 ataaatgtat cttcatcata tattcctcgt tctagtaaat catataactt atttttttgc
    54781 ttgcctaatt cttttaattc agtttctaag ccctttaaga ctcgcttttg aaattctata
    54841 ttactatctt tattattttt atctaagtct aaatcagatg cttttaattg atattttttt
    54901 acagtgtctt ccaaagattt caaaatggca gcttcaataa attcaaatcg agaactttta
    54961 ttgcaaccag gatgataaca aattatatag tcatggtctg catatggtct atatactaat
    55021 ggtctaccac aatttttgca aatcactatt cctgcaagtg gattttttat ttgattacca
    55081 tagctgacat gaccattttt tttaagtata ttttgagcct ttttccatgt tatttcactt
    55141 attaaaggtt catgttttcc ttttgcttca atccattcat ctttaggtct tagtttaact
    55201 gtttttattt tatttggatt tttagatttt ttatagtcct ttttttgcca ctgaatatat
    55261 ccacaataaa ctttgttttt tattatattt gtaattgcag aattgtacca attagcacct
    55321 gtagcagttt taatgcccaa gttatttagg tattttgata ttttactaca acccatatct
    55381 tcattaatat ataagtcaaa gatagtccta actacatcag catccttatt tggtacaagt
    55441 attctttctt ttctaccagt tgttacagca tcatatccaa atggagcaga ggttcctatg
    55501 aaattccctt cttcaactga ttttattctt cctctttgca ttcttcttga tataagtttt
    55561 aattcttttc ttgccataaa tgcttcaaat tctgaatact cctcatcaaa ttcatctgtt
    55621 aggtcgtatg tttttctagg tgtgataatt ttggtcttac tttctttgaa tgtttctaat
    55681 atgatacctt ggtctttcat gtctccacga ccaagtctgt ctaagtccat tactaagact
    55741 gcatcatatt tattttcttc aacttctttt agaagttcaa gcattttggg tctatgtatt
    55801 atactttcac cagaaactaa ttcttctttt atctcaatta catctaagtt ttgttctttt
    55861 gctagtttta gtaaggttga tttatgtcta cttaaagttt caaattcgcc ttgtttttct
    55921 gcctcttcat cagcacggga ttttcttaag tatatggcaa cactcataat aagtaatcac
    55981 actccttagg tactattata tattataatt tagagtttta ataatatttt tatatgtact
    56041 tattttaatt ataatagaat ttaaacaagt gtttttaatt atataattta ccataatatg
    56101 atataataat tgtagcaaga agatttaact tacaatctat agagtggagt tcgtgtacaa
    56161 aagattatcc tcccaacgat tcgaagggag gtgagttcat ggataacttt ttgtttaatg
    56221 ttttagctag tttaacagct agtgtagtag tttacttaat cagtaaacta ttcaaaaaag
    56281 caaaaagcca ctctcgcaca aagagtgact tacgagttga atttaaattt atatttaaat
    56341 ccaaaaaata aactctgttt atgatgaact ccactctaac gcaaaataga ttgtagttct
    56401 tcttgctttt attatatcac aaattagtac agatattcaa aaataatatt ttttttgata
    56461 taataaaaat gtagagattt tgcagtgagc gatatttttg ataaaatagg gcttaacagt
    56521 tgagatatga ggcattgagg gtatgtgata aatcttatca attgcactac tcatggttca
    56581 ctgcaaattt gagagagttg tatatgtgta gatattgaaa atacttagtt tattttgggg
    56641 ttttatatta actaagtggt atgtaaagaa tacatcatta ggagtacact caagagctag
    56701 gcaaagtttt atattaacta agtggtatgt aaagtcatct cttgcaacaa cttaacttca
    56761 tcatctttaa gcgttttata ttaactaagt ggtatgtaaa gaatgatata tcaggagtag
    56821 tatatattgt aatggattca gaaggagcgt gggaatttaa gattgcaaag gaaatggaag
    56881 agtctgaata tcagatagac ttaaataaaa tcttataaaa taaataaaat agagtggtcc
    56941 atgactactc tattttattt ttagtgttta tatattattt atctcttttt ttcaactctt
    57001 catctataaa atgacaagct aataatagcg gactgatttc taagccaatt gacaaatcaa
    57061 ctatagttgg tattgtaata tttttgtatt tgtttctttc taaatcagat atatactctc
    57121 gactatagtt agttctattt gctaattctt tttgtgttaa acctcttaaa atccttaaat
    57181 cttttaacat gcctgtttcc tcctaaagta ttatagataa tctatttttt acatatagaa
    57241 aaaaacaatg cggttaatag caacattttt tttgtggaaa tttgtggtag aatgtagtta
    57301 agaatattaa gaaaatttat tttggaggtc tataaatacc gaccaaataa tttgtaaatt
    57361 atatgtaaaa ataatgcaaa aaattgtaag aaaaaaatca aaaataaagt aatttattta
    57421 agtgattccg ataaactgaa aagaaattct cataaaccaa aaataaattc tgataaatcg
    57481 aaataaatgt tatgatttct taaaattaaa actaaaatct caaaagtaag caagttgcaa
    57541 gataatatta aaaaatatat aatt
//
LOCUS       DAYAPA010000009        52753 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.009, whole genome shotgun sequence.
ACCESSION   DAYAPA010000009 DAYAPA010000000
VERSION     DAYAPA010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 52753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52753
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.009"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(72..845)
                     /locus_tag="ACGI59_000474"
     CDS             complement(72..845)
                     /locus_tag="ACGI59_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigB/SigF/SigG family RNA polymerase sigma
                     factor"
                     /protein_id="HFG9738287.1"
                     /translation="MKNVANATHYLNMDTKELFNLYNKNKNVDIRNILIERHLYLARL
                     LAKKYINKGVDFEDIYQVASLALIYAIDRYDVEKGFEFSSFATPTIVGEIKKYFRDKV
                     WTLRVPRRIQELSKKISDAKIKLEQENKKHPKVKDIADYIGVSEEDVLEAMEASYGYQ
                     PMSLDSSSNDDSEDKDITLIDKIGKEEGNFGSIEYEDFINKFIETLNELEVKIFKDRF
                     FFDKTQSSIAKELGISQMTVSRLEKKVVEKLKKEYEKNL"
     gene            complement(845..1252)
                     /locus_tag="ACGI59_000475"
     CDS             complement(845..1252)
                     /locus_tag="ACGI59_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFG9738288.1"
                     /translation="MACETIKMEITTNPDYVSIIRLTASGIANKMGFPIDDIEDIKVA
                     VSEACTNAIKHSKDDVFNIVFNILDNAINIEIQDNGKGYDVSSISTPDLENPKESGLG
                     LFIIKTLMDDVDIESEHNQGTKIKMTKYLGVDI"
     gene            complement(1256..1573)
                     /locus_tag="ACGI59_000476"
     CDS             complement(1256..1573)
                     /locus_tag="ACGI59_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HFG9738289.1"
                     /translation="MSMNIDSNLDSQNKFWNVCLDGELDVSTADKLKEHLHALIEKNM
                     LDVKINLKDLDYIDSTGLGAMIGVLKKLKINEKEIYIVNPKSNVRKIFTITGLDKIFK
                     VEG"
     gene            complement(1585..1845)
                     /locus_tag="ACGI59_000477"
     CDS             complement(1585..1845)
                     /locus_tag="ACGI59_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HFG9738290.1"
                     /translation="MNAWGWQIGAVLVGSSALIVAIYLAKTLNSINKVVEKAYKIVDY
                     NERHIQDIVENASSISKGIDDIVYVINKILSIGNIFKIIKRK"
     gene            complement(1845..2141)
                     /locus_tag="ACGI59_000478"
     CDS             complement(1845..2141)
                     /locus_tag="ACGI59_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFG9738291.1"
                     /translation="MCNKRSCKKGGFLLLGAILGFIFGMFFAPKKGSELRKETKEKFN
                     DVKENPKEVLHETFNDVKERIINLVDDDNNEEDIKISEEDIVISKSFDDEGDVN"
     gene            complement(2210..4636)
                     /gene="gyrA"
                     /locus_tag="ACGI59_000479"
     CDS             complement(2210..4636)
                     /gene="gyrA"
                     /locus_tag="ACGI59_000479"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HFG9738292.1"
                     /translation="MEENNKILPIEIAEEMKKSYIDYSMSVIAGRALPDVRDGLKPVH
                     RRILYSMSELNLTPDKPYRKSARIVGDVLGKYHPHGDTAVYYAMVRMAQDFSTRALLV
                     DGHGNFGSVDGDSPAAMRYTEAKMSKLSLELLRDIEKETVDFKPNFDESLKEPSVLPA
                     RYPNLLVNGSNGIAVGMATSIPPHNLAEVIDATVYLIDNPECSVDDLIKFVQGPDFPT
                     AAIIMGKESIAEAYRTGRGKVKVRSRAFIEELPKGKQQIIVTEIPYQVNKAKLVERIA
                     ELVKEKRIEGISDLRDESNRNGMRIVIELKRDANANIVLNNLYKHSQMEDTFSIIMLA
                     LVDGQPRVLNLKQILYHYIKHQEDVVTRRTKFELNKAEARAHILEGLKIALDNIDAVI
                     SLIRASKTGQEAKLGLIEKFKLTEIQAQAILDMRLQRLTGLERDKIEAEYEDLIKKIN
                     RLKEILADERLLLNVIKDEITIIKENYSDERRTEIRHAEGEIDMRDLISDEEIAITLT
                     HFGYIKRLPSDTYKSQKRGGRGISALTTREEDFVRHLVTTTTHSRLLFFTNKGRVFKL
                     NAYEIPEGKRQAKGTAIVNLLQLSADEKIATLIPIDGNDENEYLLLATKKGIVKKTKR
                     EEFKNINKSGLIAIGLRDDDELIGVELTDGKQEVLLVTKEGMSIRFDENDIRYMGRTA
                     MGVKGITLSKEDFVVSMNLCSKGTDVLVVSKNGFGKRTNIEEYRSQIRAGKGIKTYNI
                     SEKTGTIVGADMVNEDDEIMIINSDGVLIRIRVNEISLFGRVTSGVKLMKTNDEVNVV
                     SIAKINIEEE"
     gene            complement(4656..6557)
                     /gene="gyrB"
                     /locus_tag="ACGI59_000480"
     CDS             complement(4656..6557)
                     /gene="gyrB"
                     /locus_tag="ACGI59_000480"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFG9738293.1"
                     /translation="MKQEYGASQIQVLEGLEAVRKRPGMYIGSTSPRGLHHLVYEVVD
                     NSIDEALQGYCSDIYVSINEDGSVLVKDNGRGIPVEIHPKTGKSTLETVLTNLHAGGK
                     FGGGGYKVSGGLHGVGVSVVNALSKWMVAEVYLNGKIYKQTYEKGLPTSKLEVVGESQ
                     DKGTMIQFMPDETIFDEIEFKYETLEYRLRELSFLNKGIKIVFEDKREGQEKRKEFHY
                     TGGLVEYIKYLNKSRTGIHDDIVYIDKKVDDCFVELAMQYTDGYTENIYSFANNINTH
                     EGGSHLSGFKAALTKTVNDYAKRNKFLKENDVNLLGEDIREGLTAVVSVKLPEPQFEG
                     QTKTKLGNSFMRGIVDSVTVDELGSFLEENPSTARIIVDKALRAQRAREAAKKARELT
                     RRKSVLESTSLPGKLADCAEKDPSKSEIFLVEGDSAGGSAKQGRDRNSQAILPLRGKI
                     LNVEKSRLDRILSSDEIKNMITAYGCGIGEDFDIDKARYHKIIIMTDADVDGAHIRTL
                     LLTFFFRYMRPLIDEGYVYAAQPPLYKVTKQKKEHYVYSDKELNILLDEIGRNGVELQ
                     RYKGLGEMNAEQLWETTMNPETRTLLQVTVEDAAIADEVFSMLMGDKVAPRKEFIEEN
                     ARFVRNLDI"
     gene            complement(6558..7673)
                     /gene="recF"
                     /locus_tag="ACGI59_000481"
     CDS             complement(6558..7673)
                     /gene="recF"
                     /locus_tag="ACGI59_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFG9738294.1"
                     /translation="MKLKSLQLVNFRNYKKLHLEFNGKVNLLVGKNGQGKTNIVESIY
                     MLSFGKSFRTNKDKEMVRFNSENLYIGGSFSKYNKYSLIELIIGKDKKGIRINKVPLQ
                     KIQELLGNLNVVIFSPEDLRLVKEGPKERRAFIDKEISQIIPKYYKYLTNYNKTLSQR
                     SRVLKNIHVDEALLDVYDDTLAKYGSYIYILRRDFIKKIANISENMHMNLTNGVERLS
                     IRYKNQINITDEDTIDTVYNKFLAKLSSNRPNDIESKTTRYGIHKDDLNIFINDLDAR
                     LFGSQGQQRTASISLKLSEIELIKNEVEEYPVLILDDVFSELDEARQKLLVNNLSNVQ
                     MFITSAEVSHKKIFDEKNVTIFNIENGDVISIENGGN"
     gene            complement(7691..7897)
                     /gene="yaaA"
                     /locus_tag="ACGI59_000482"
     CDS             complement(7691..7897)
                     /gene="yaaA"
                     /locus_tag="ACGI59_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HFG9738295.1"
                     /translation="MTEITIESEYIKLDQFLKLAEIASTGGHAKFLIQEGLVTVNDEI
                     ELRRGKKIKSGDIVEIEGTKIKVL"
     gene            complement(8034..9140)
                     /gene="dnaN"
                     /locus_tag="ACGI59_000483"
     CDS             complement(8034..9140)
                     /gene="dnaN"
                     /locus_tag="ACGI59_000483"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFG9738296.1"
                     /translation="MKIICNQKILANRIGIAQKAINGKTTIELLKGILISTEEGQLKL
                     TGYDAEIGIETYVQAEIIEKGDVVVDARLFGDIIRKLPDSFVEIETDSENNIYINCVN
                     SRFKIKGYAAKEFPKLPELNEEDLYSIPQEILKNMIKQTVFAISQDQTKPVLMGELLE
                     IVDRNLNLVAIDGYRLAVKSCSVDSLTENIKVIIPGKTLIDVNSLLSGEDNVKVGFNE
                     KNAIFIINDTKIITRLLEGDFIDYKKLLPREHNSRVKLNTKELLNSIERASLLSQSEK
                     NNLIKLSIRDKVMAITSNTEKGNVYEEVEIDLDGDYLDIAFNSRYFIEGLKNIDNEEI
                     FIEFTTNVNPCIIKPTDDVNYIYLLLPVRISSNI"
     gene            complement(9382..10701)
                     /gene="dnaA"
                     /locus_tag="ACGI59_000484"
     CDS             complement(9382..10701)
                     /gene="dnaA"
                     /locus_tag="ACGI59_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFG9738297.1"
                     /translation="MDIVSLWDKTLQLIKGDLTSVSFNTFFKNIVPLKIHLNDLILLA
                     PSDFNKDILENRYLHLIEDAISQLSLKKYNIKFVLSEKEVADLNSDSTDLNYRVLYPN
                     LNPKYTFDTFVIGNSNRFAHAACVAVAESPAKAYNPLFLYGGVGLGKTHLMHAIGHHI
                     VSQKKDSKVVYVSSEKFTNELINSIKDDKNEEFRNKYRNVDVLLIDDIQFIAGKERTQ
                     EEFFHTFNTLHEANKQIIISSDRPPKDIPTLEDRLRSRFEMGLITDIQAPDFETRIAI
                     LRKKAQLERIDVPNEVMSYIAKNIKSNIRELEGALTRVVAYSSLSNRVISFDLATEAL
                     KDIITTSKNEEINVLRIKEKVSSVFNLKMEDFNSKKRTRSIAYPRQIAMYLTRELTDL
                     SLPKIGEEFGGRDHTTVIHAHDKVSKDIEESEEIKTKIDKIISDLKG"
     gene            11360..11497
                     /gene="rpmH"
                     /locus_tag="ACGI59_000485"
     CDS             11360..11497
                     /gene="rpmH"
                     /locus_tag="ACGI59_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFG9738298.1"
                     /translation="MSKRTYQPKKRQRSKEHGFRKRMKTSNGRNVLKRRRAKGRNRLT
                     H"
     gene            11575..11919
                     /gene="rnpA"
                     /locus_tag="ACGI59_000486"
     CDS             11575..11919
                     /gene="rnpA"
                     /locus_tag="ACGI59_000486"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFG9738299.1"
                     /translation="MDFNRTKGLKKDSDFRKVYKHGKSFANKYLVIYILKNKSDYSRV
                     GISVSKKVGKAITRNRVRRLIKEAYRLNIDEKIKPGYDIVFIARVSSKDATFKDIDKS
                     IKNLVKRTDISI"
     gene            11951..12202
                     /gene="yidD"
                     /locus_tag="ACGI59_000487"
     CDS             11951..12202
                     /gene="yidD"
                     /locus_tag="ACGI59_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFG9738300.1"
                     /translation="MNKIIYILKEISKYLSNLCIYLVRFYQKYISPLKGPTCRFYPTC
                     SQYSIEAFKKYGFVKGMYLTIRRILKCHPFHPGGYDPLK"
     gene            12234..12941
                     /locus_tag="ACGI59_000488"
     CDS             12234..12941
                     /locus_tag="ACGI59_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HFG9738301.1"
                     /translation="MNIIGNFLGLILKVIFEFVNHYGISIILFTILVKIILLPLTIKQ
                     TKSTKAMQDIQPRIKEIQEKYKNKPEKQNEEIVKLYGEAKINPLSGCLPLLIQFPILI
                     GLFSVLREPVAHGVFANKAAFLAADNGFLWIKSLTSPDYVLAVFSGASAYVMQKVMTP
                     KDQLQGSMKVMTYVMAGMSFYWGFIFPAGLTLYWTVSNLFSIAQYYLIMNPLKAKLAA
                     NSKEEKVNEKKPKINKK"
     gene            12910..13539
                     /gene="jag"
                     /locus_tag="ACGI59_000489"
     CDS             12910..13539
                     /gene="jag"
                     /locus_tag="ACGI59_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HFG9738302.1"
                     /translation="MRKSLKLIKSKSKEEAISKAIAELNLKAEDIEVEILENPSKGFL
                     GFIGAKDGTYEIFVIEREVDVAKNFIEVMLKNANVDAKVNVSQKDNLIMVDIEGKEAA
                     SLIGRRGETLDSIQFLTGLALNKINKDSHTRVLVDIENYRSKREESLIRYANKVAREV
                     AKTRKTKKLDYMNPYERRIIHSALQNDKYVITYSEGTDPYRRLVIECKR"
     gene            13651..15030
                     /gene="mnmE"
                     /locus_tag="ACGI59_000490"
     CDS             13651..15030
                     /gene="mnmE"
                     /locus_tag="ACGI59_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFG9738303.1"
                     /translation="MFIDDTIAAIATAPGEGGIGILRISGEKALKVAEEIFKSMSGKS
                     IEEYNKRTLIYGNIVDNENIIDEVLLAYMKGPNSYTGEDVIEINCHGGFISVKKILEL
                     ILSKDVRLAEAGEFTKRAFLNGRIDLSQAEAVIDVIKAKTDIAHEVAQNQLEGSLSKK
                     IRELRDKVTEILAHVEVAIDYPEEDIEHITYQTLKEKTDELKKDIKKLYDTAESGKIL
                     REGLKTVIVGKPNVGKSSLLNSILGENRAIVTDIPGTTRDVIEEFVNIKGIPLKIVDT
                     AGIRDTDDIVEKIGVEKSKESFTSADLIVMVLDASRKLSEEDIEILEKLKDKQTIVLL
                     NKNDLKQEIEEEKILKYVENNSIIKISALQQEGIEELQDKIESMVYKGSIKNNSSLVV
                     TNSRHKDALSKAYKSATDALIALEQSMPFDFVEVDLKNIWDYLGYINGDTVTEDLLDN
                     IFHNFCIGK"
     gene            15125..17020
                     /gene="mnmG"
                     /locus_tag="ACGI59_000491"
     CDS             15125..17020
                     /gene="mnmG"
                     /locus_tag="ACGI59_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFG9738304.1"
                     /translation="MIKFEAGKYDVIVVGAGHAGCEAALATARMGYKTLIITMSLDSI
                     ALMPCNPSIGGTGKGQLVKEIDALGGQMGLNIDKTYIQSRMLNTAKGPAVHSLRAQAD
                     KFKYHEEMKKTLEDEPNLDIAMDEVVEILHEGNVVIGVGTKLGCSFKSKAVILATGVY
                     LNSKIYMGEVAFYEGPNALGYAKYLTDSLVELGLRMRRFKTGTPARVHRDSIDFSVMS
                     LQEGDEKVTPFSFMNENIEKKQEPCYLTRTTEETQKVILDNLKRSAMYSGVIESTGPR
                     YCPSIEDKVVRFSDKTSHQLFIEPEGLNTKEMYIQGISTSLPFEVQLDMYKTIKGLEN
                     CKIMRPAYAIEYDCVDPTQLKISLEIKGVENLFSAGQFNGTSGYEEAAAQGLMAGINA
                     VRKIEGKEPFVLDRSEAYIGVLLDDLVTKGTNEPYRMMTSRAEYRLYLRQDNADMRLT
                     QKGYDIGLVKKDRYERFLNKKAAVEKEFERLKNERVTPKEVNSLLEEKGATPIKVGIS
                     LYEFLKRPEVTYELLEELGKGAGEDVSREVKEQCVIITKYEGYIEKQLKQIDQFKKLE
                     NKKLDEKINYSSIEGLRLEARQKLDDIKPISIGQASRISGVSPADISVLLIYLEQIRR
                     TRGGKGE"
     gene            17013..17732
                     /gene="rsmG"
                     /locus_tag="ACGI59_000492"
     CDS             17013..17732
                     /gene="rsmG"
                     /locus_tag="ACGI59_000492"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFG9738305.1"
                     /translation="MNNIELLKNGIEGFNIDTNDSMLEKFKIYREILVDWNKKMNLTG
                     IEDEKEVYIKHFLDSISAVKNGYIKNGMSIIDVGTGAGFPGIPLKICLDSLELTLLDS
                     LNKRINFLEEVSRVLELDNITFIHGRAEDFGKDEDYREKYDVATARAVAGLPILMEFC
                     VPFVKVGGYFICLKGPNANLELEESQKAIDVLGLEYIEKIDVELPEVDLNHNILVFKK
                     VMETPVKYPRKAGKPAKNPIK"
     gene            17895..18680
                     /gene="noc"
                     /locus_tag="ACGI59_000493"
     CDS             17895..18680
                     /gene="noc"
                     /locus_tag="ACGI59_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0032466 - negative regulation of
                     cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion protein"
                     /protein_id="HFG9738306.1"
                     /translation="MEDKRVMEIPIEDVVPNPYQPRKIFSQVSLEELSNSIKVYGIIQ
                     PITVRAKDGKYELIAGERRLRAAKLAELKTIPAIINNMNDESSAVLALLENLQREDLN
                     FIEEAIGYENLIKEHAFTQQQLAEKLGKNQSTVANKLRILKLPNDIKMKLIENNLTER
                     HARAFLKLPSEDLMQSVLDKVIKNELTVKKTEKLIQDVLEETKVQEEPDKKQNIKGAM
                     SIRIYINTIKQAFDAISNTGIDAKYNEIDKGDYMEVVVKIPKK"
     gene            18815..19588
                     /locus_tag="ACGI59_000494"
     CDS             18815..19588
                     /locus_tag="ACGI59_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFG9738307.1"
                     /translation="MGKVIAVFNQKGGVGKTTTNVNLSASLGTLGKKILVLDLDPQGN
                     TTSGYGINKNEVENTIYEIMLDGLHIKEAIISTEFENVDVVPSATELSGAEIELTSKT
                     NREYILKNSIKAVIDEYDYIFLDCPPSLGMLTINCLTAVDSVLIPIQCEYYALEGVSQ
                     LMETIKLVKSRLNADIEIQGVVLSMFDGRANLSIQVVEEVKKYFKGSVYTTLIPRNVR
                     LAEAPSHGKPVIYYDKRCRGSVAYLELAEEFIDLEEEEW"
     gene            19590..20453
                     /locus_tag="ACGI59_000495"
     CDS             19590..20453
                     /locus_tag="ACGI59_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFG9738308.1"
                     /translation="MENKSKRSNRLGRGLSALIPEIKGETSEKEIVNIDIDKIYPNEV
                     QPRKQFDEEKIKVLSDSIKNYGVLQPIVVKMDENNKYMIIAGERRFRASKLANKNQIP
                     AIIKDIDMKDIMEIALIENLQREDLNSIEEALAYKSLIEHYNVTQEEISEAVGKSRPH
                     ITNTLRLLNLGQDVIEMIDSGRITAGHGKALLRIADKDLQLQIAKKIEEEELSVREVE
                     NIAKKISENKQEEPKKSKPKDVFILDVEDKLRNIFGTKVNISKGKKKGKIEIEYYNDD
                     DLNSIVSMLLE"
     gene            20486..21628
                     /locus_tag="ACGI59_000496"
     CDS             20486..21628
                     /locus_tag="ACGI59_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HFG9738309.1"
                     /translation="MIYLDNAATTYPKPERVYNAVLDCMKNYCANPGRAGHKLAMRAA
                     REIYDTRENIAKLFNVSNPMNIVFTSNATDSLNLAIKGVLQEGDHVITTSMEHNSVIR
                     PIKALEKRGIENTVVKCDYEGFLDYEDLEKSIKSNTKLIVTTHASNVCGTLIDIKKVG
                     EIAKKHNILFLVDASQTAGVYDIDVNECNIDMLAMPGHKCLFGPQGTGILYVREGLNL
                     NILKEGGTGSKSEEIVQPELFPDKYESGTHNTPGIAGLNQGILFIFERGINNIRQHEE
                     ELCQYMIDKLEEVPDIKIYGPKDSKKRASVIALNIGDMDSGEVTFLLDSDYNIATRSG
                     IHCSPLAHTTLGTLKQGAVRFSIGYFNTKDEIDKAVEALKKISKNK"
     gene            complement(21762..22337)
                     /locus_tag="ACGI59_000497"
     CDS             complement(21762..22337)
                     /locus_tag="ACGI59_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HFG9738310.1"
                     /translation="MKNKKIMLVLSILSISIFAVGCTNAQNGSDSSKKETKSNTNVEQ
                     PKEENNTEKEVPNNKAKPTPKEETKTQSATIYSFDVDKTDLIENKVDLNKIDENTLFE
                     ELQKLKVVPESAKLNSFTTKDIDGVKTGILDVSSDFTKSNLGSDAETLMLDSVARTYI
                     KNMNVEQIKITVDGSNYESGHIVLEEGDYLK"
     gene            22597..23889
                     /gene="rstA"
                     /locus_tag="ACGI59_000498"
     CDS             22597..23889
                     /gene="rstA"
                     /locus_tag="ACGI59_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin production/motility transcriptional
                     repressor RstA"
                     /protein_id="HFG9738311.1"
                     /translation="MEILSLGEKIKKLRKEKNMTLKELAGDRITAAQISHIERDKSHT
                     SYELLEYLGDKLDVSVEYLLETKEMQSKKITDNLILQSEIYIKENELNKAEDQIKEVL
                     DICKRYDLMENYGRCNFLMANINLKKSNYNDAIENFEKALYFFIKNSDSENILKCYLN
                     IGKIYMQEDFYKGAISHFNFAEEILSENKFEDVNIHKELYSKMAYCYIKLDNPNMSLK
                     YIEKINEIDNRNDRKEDVDILVLKANNMLEIGKYEESRDYFKKALKILESEDNKTGLA
                     NVYLTICDVYRKIGETDRVLEYSQKVYDIKKNDEDEYMMSGLFKIIEAYIDKEDYDLA
                     RKYCKIALASSIKNKNKFNEYKALRFYANMYKNQNEVTLAIEYLIKCIKIVSDLGNNR
                     ILANLYIDLGQLYSNISKEKELEYYQKGVFMYKNLEIM"
     gene            23994..24596
                     /gene="yedF"
                     /locus_tag="ACGI59_000499"
     CDS             23994..24596
                     /gene="yedF"
                     /locus_tag="ACGI59_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HFG9738312.1"
                     /translation="MSIKIDARGLACPKPVINTKRELDNLEEGVVVTIVDNETAKENI
                     LKLAKSLSCEANIVDEKENFISIEIRKGKNILDKQNVVDNKESELDNTCIFISSDKMG
                     LGNDELGKVLIKGFIYTLTESKPYPKYVLLVNGGVKLSAENEETIENLKILENMGVEV
                     LSCGTCLDYYNLKDKLQVGTVTNMYTIVETLKNASNTISI"
     gene            24669..24932
                     /locus_tag="ACGI59_000500"
     CDS             24669..24932
                     /locus_tag="ACGI59_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HFG9738313.1"
                     /translation="MNQMYIVSFNSTHHAIRSEKLFGENSLKVMALPTPREITASCGI
                     SIKFSFEDMEKIKTILVENNVDIKGIYCISKLENGSKEVEKLD"
     gene            24947..25132
                     /locus_tag="ACGI59_000501"
     CDS             24947..25132
                     /locus_tag="ACGI59_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HFG9738314.1"
                     /translation="MPMDLKIGDIVELKKQHACGCKEFEIIRTGMDIKIKCTKCSRLI
                     MLDRETLEKRVKKLIKK"
     gene            25235..26431
                     /locus_tag="ACGI59_000502"
     CDS             25235..26431
                     /locus_tag="ACGI59_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFG9738315.1"
                     /translation="MAVKYAKRMQGLQGSEIRELLKLTQQPQIISFAGGMPAPELFPV
                     EEMKKVSVAVLEENGRSAMQYTTTEGYEPLREKIAARMNDKNKTNVNKDDILVTSGSQ
                     QGLDFAGKVFIDEGDVILCESPSYIGAINAFKSYQPKFIDVPTDSDGMIMEELEKILE
                     TTDRIKMIYVIPDFQNPTGRTWPLERRKKFMEIVNKFEIPVIEDNPYGDLRFEGETLP
                     SLKSMDTKGLVIFLGTFSKIFCPGYRLGWTCASPEILSKFNFAKQGADLQASTISQME
                     VSKFMDMYDLDAHVDKIKAVYVKRRDVMLKTMEEEFPEGLVFTHPEGGLFTWVELPSN
                     LNAKELMPKCLDKNVAYVAGGGFFPNGGRENTFRLNYSNMPEEKIIEGIKNIAAVLKE
                     AMGVEA"
     gene            26645..26923
                     /gene="rpsF"
                     /locus_tag="ACGI59_000503"
     CDS             26645..26923
                     /gene="rpsF"
                     /locus_tag="ACGI59_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFG9738316.1"
                     /translation="MRNYELVYVVKPNSDEEVREAILNKVKEVVATDGEIVKVDTWGT
                     KKLAYPIAKFTEGFYVLVNFKSAVDVPKEIDRNLKINENVIRHMIVVA"
     gene            26960..27394
                     /locus_tag="ACGI59_000504"
     CDS             26960..27394
                     /locus_tag="ACGI59_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFG9738317.1"
                     /translation="MNQVVLVGRLTRDPELRYIPGTGTPVASFTIAIDRDYVKKDGSK
                     ETDFIPVEVMGKSAEFCANYITKGRLVALQGSIRVDNYQTQSGEKRTFTKVSTRSVQA
                     LDSKNKSENSYRESAPAFEPSFEPQGLDPQGFQAIDDDDIPF"
     gene            27413..27640
                     /gene="rpsR"
                     /locus_tag="ACGI59_000505"
     CDS             27413..27640
                     /gene="rpsR"
                     /locus_tag="ACGI59_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFG9738318.1"
                     /translation="MMNKKRRKKKRVCQFCADKNAKIDYKSTQRLQKYITERGKILPR
                     RISGTCAKHQRELTVAIKRARNIALLPYTLD"
     gene            27783..28103
                     /locus_tag="ACGI59_000506"
     CDS             27783..28103
                     /locus_tag="ACGI59_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HFG9738319.1"
                     /translation="MKIDKGSEITRNIKIIEWMKTEILMSVSDLFNLLFKGVKPLDEA
                     IQDTLANIIMITYLLAKRLGISFRDVDYKVKEKIKIGIDEDHSVERWYGDLSNLKKHM
                     DNRE"
     gene            28111..29067
                     /locus_tag="ACGI59_000507"
     CDS             28111..29067
                     /locus_tag="ACGI59_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YybS family protein"
                     /protein_id="HFG9738320.1"
                     /translation="MNNKIRLSKAMSIIVLAIIIALVIAYIPMLGIFSMLAAVPYVII
                     GAITDRKYSLISILVTFCVLILFADISYALNICIMYSLPGIVIGKMLKRSFEQEDSNK
                     FEPIYGGTIIFVLSMLVYFFVLKTFLNVNLLDEVSKMISEIVNIQKNSFSATELKVFD
                     KMKPDEIVSYFTNMLPMMLFLQGLLSAFVTYYLSVFFIKRITKMNIRFPKFADFYLPG
                     NAILTTFLLYLLVLFIQIIGSKLYTELIMTNLQLVFNLMFVIQGIAVCIYFLKKWIRQ
                     GPNKIMFFSGIILCLFGFMGISFVGMVDSIIDFRKVRSYKST"
     gene            29078..31072
                     /locus_tag="ACGI59_000508"
     CDS             29078..31072
                     /locus_tag="ACGI59_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HFG9738321.1"
                     /translation="MSNKQTFKLNMPEINLYIIVIGISSIILLYYNLYVGCLFFCIFV
                     YMVFHNWRTTNIRRHEWTEYIQNLSLDIDETTKKAIINLPIPLCILEFDGNISWYNGK
                     FYDMIGQKDLLDKNIEDIVKNLNLRKVLNENKEMYTEINYKEKEYTIIYNVIKNDQEK
                     NPKYLMILYWIDKTEYLKVKQNYDDEKNAMMLIQVDGYDEVLKSAAEDKRALINVEVE
                     KILSALELNSNGALRRTSKDKFFLVMHKKELKKLEAEKFSILDTIRHIDYGNNLPVTI
                     SIGIGIDGDTLNENLKLATGALDLALGRGGDQAVVKTKDKFVFYGGKSKAVEKKTKVK
                     SRLIGHALREVIQQSDQVYIMGHKYPDMDAMGAAVGVYDICKSCNKTANIVLQSVNES
                     IEIFINKINENNYYKKLFIGKEEAIDNCTKNTLVVVVDTHRPNYTECEELLKLSEKVV
                     VIDHHRRGVEFINDAVLLFHEIYVSSTCEMVTELVQYMDEDVTINKLTAEGLLAGISL
                     DTKNFAFKTGVRTFEAASYLRKVGADTIEVKKFFNSDVKDFIIKAEIIQSTKIINNRI
                     CLAYSSTEIDSINVIIAQTADELLNIKEVEASFVLGEKDDTIFISARSLGQINVHVLM
                     EKLGGGGHIDIAGAQLKNVSLKEAYKMVNKIIEEYLEEEE"
     gene            31073..31522
                     /gene="rplI"
                     /locus_tag="ACGI59_000509"
     CDS             31073..31522
                     /gene="rplI"
                     /locus_tag="ACGI59_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFG9738322.1"
                     /translation="MKVILLKDVKGTGKKGEMKEVSDGYARNFLFPKKMAVQADSVAI
                     KELNEKNKSKEIKAQKEYEEAVLLGKQMEEINIEIYSKAGEGGRLFGSITSKEIAEQL
                     KKQKDIDVDKRKILLDEPIRSLGSTFVEIKIHQKVTTKIRVDVKEKQ"
     gene            31541..32869
                     /gene="dnaB"
                     /locus_tag="ACGI59_000510"
     CDS             31541..32869
                     /gene="dnaB"
                     /locus_tag="ACGI59_000510"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFG9738323.1"
                     /translation="MEDMTRIPPHSVESEQSILGSILLDKDAIITVTETIKPDDFYKE
                     AHKIIYECMITLSNKGEPIDLITLTEELRKQGHLNDIGGISYITSLSTIVPTTSNVKY
                     YADIVKEKSVLRKLIKASNEIINLGYSGATKIEDVLEQAEKSIFDISQEKTSDDFKSI
                     NLVLMDAYDMIEKLYTNKSDVTGITTGFKDLNKKINGLQRTDLILIAARPAMGKTAFS
                     LNLVQNAALKGDASVAVFSLEMSKEQLVQRMLSSQSSVELKKIKTGTLNDNDWPRIID
                     AMAVLSDAKIHIDDTPGIKISELRSKCRKLKIEKGLDLVLIDYLQLMEGEGNNESRQQ
                     EISKISRSLKILAKELNCPVVALSQLSRAPEQRADHRPMLSDLRESGAIEQDADIVMF
                     LYRDEYYHADSESKNIGEVIIAKNRHGETGSVELVWLGEVQRFGDKLRDL"
     gene            33074..33382
                     /locus_tag="ACGI59_000511"
     CDS             33074..33382
                     /locus_tag="ACGI59_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9738324.1"
                     /translation="MEKISLKYIYPNIIKVLDEINLFRVIDNNLRESIVVYANNVDNQ
                     YHINMTNTNFGNIINICKLEKLLDVDKFMEKVIKYEKEIIEKEEFSKIEEYMLNIGEY
                     "
     gene            33464..34753
                     /locus_tag="ACGI59_000512"
     CDS             33464..34753
                     /locus_tag="ACGI59_000512"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFG9738325.1"
                     /translation="MKTVAIVGSQWGDEGKGKVIDYLATQADVVVRGQGGNNAGHTLV
                     VEGKKYALHLIPSGVLNPNTVNIIGNGIVFDPKGFLEELEMFKTDNISTENIKISDRA
                     HVIFPYHKELDALSEEARGDLKIGTTKKGIGPCYMDKTERSGIRICDLMDKDKFAIKL
                     KAQIDAKNEIVKNIYGKEELFDFETIYNEYLGYAEQIRKYVADTSVIVYDAVRAGKKV
                     LFEGAQGTLLDLDLGTYPFVTSSHPTSGGFAIGAGIGPNMIKDVVGIVKAYTTRVGEG
                     PFVTEQINETGDKIREQGHEFGVTTGRPRRCGWFDAVIVKYAARVNGLTSISFMLLDV
                     LTGFDKIKVCTSYKMGDKIITDFPASLDDLAKCEPVYEELDGWNEDITQIDNFDDLPE
                     NAKKYVAKIEELVGVSVDMVSVGPNRAQTIIRRNIFA"
     gene            35079..35154
                     /locus_tag="ACGI59_000513"
     tRNA            35079..35154
                     /locus_tag="ACGI59_000513"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35112..35114,aa:Lys,seq:ttt)
     gene            35161..35235
                     /locus_tag="ACGI59_000514"
     tRNA            35161..35235
                     /locus_tag="ACGI59_000514"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35194..35196,aa:Glu,seq:ttc)
     gene            35240..35315
                     /locus_tag="ACGI59_000515"
     tRNA            35240..35315
                     /locus_tag="ACGI59_000515"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35273..35275,aa:Val,seq:tac)
     gene            35349..35423
                     /locus_tag="ACGI59_000516"
     tRNA            35349..35423
                     /locus_tag="ACGI59_000516"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35381..35383,aa:Thr,seq:tgt)
     gene            complement(35806..36798)
                     /locus_tag="ACGI59_000517"
     CDS             complement(35806..36798)
                     /locus_tag="ACGI59_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFG9738326.1"
                     /translation="MNEDKIRKILAKYAKRRDDNELLLEHRKNEVYNRIPEIKSIDDE
                     ISKIGLSLAKIVLLNPKSKDEIVKKTKENIESLKVKKERLLAESNIPLDYLEIKFQCI
                     SCKDKGFLPNGEKCSCLKQEIVNEAYKMSNLDRILSQENFSNFNLNIFSPKKGSDGEI
                     SPRENMLNNLSICENFVHDFKKDNSENLLFYGSTGLGKTYMCNCIAKELLDKGNVVIY
                     QTSFRILDILEDYKFRRDTNNQISEDNYKNLFDCDLLIIDDLGTELNNSFTSGEIFNI
                     VNTRLVAGKKIIISTNLTPSQIGNTYTQRTLSRILDKFRILEFTGDDLRWERFK"
     gene            complement(36807..37865)
                     /locus_tag="ACGI59_000518"
     CDS             complement(36807..37865)
                     /locus_tag="ACGI59_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFG9738327.1"
                     /translation="MFFKESNEIDLGEITIPNIFIDIFMPMADGLYVKVYLLGYRQAC
                     DITSNPKFDNNSIAKNLNIPLSDVLSAWKFWEEKKIIKIHDNEEYDNFNYSIEFLDLK
                     NFYIENILSNNSSIKSNTDKVVSTSENPSIRKMFNSINKIVGRYLDPSEKMSIMDIMN
                     KYNMSPDMILCAYEYVKDKTGTSKPVKYIEGIIRNWYDSNLYTPKDVEESFLVRSERY
                     ILYKTIFNELGFSRQPSKSEKELMDTWFDKFNMDIDLIINACSKSKNISNPSISYING
                     IIKNWNEKNIKNLNDLKQKEEERIVKENINKKQINTTQNNNTYKKTKFHNFNETFTQY
                     TSDELDEIIKKSQKEKFK"
     gene            37990..39453
                     /locus_tag="ACGI59_000519"
     CDS             37990..39453
                     /locus_tag="ACGI59_000519"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HFG9738328.1"
                     /translation="MVIKFNQKVRRTLSVAILFMVLIIGCFIEKDDSLETYKEKTNSK
                     INQYNMDVIFDDETKRLMCNQNVDYINNTKSNIDKIYFHIYPNAFSKKDFAPFEKSEM
                     TRAYPNGFNEGYIDIKNILNNNSKMEYKIKGDKNDILEIELGKELKPNERMSLDIKYN
                     VKIPNSIGRFGYGENTINVTNWFPIACVNDERGWNLKSYETIGDPFYSETSDFHVKLL
                     IPNKYKIAHTGKLIDDKHDNKKMLYEIEAENVRDFAFILSSKFDVNKVDSKGVAINTY
                     NLNKKLSKKATEVAKDSINIFSELFGKYPYKEFSVVASDFFIGGMEYPMLVMIDQSLY
                     NEKNEFLLEYVIAHETAHQWWYSAVGNDEISEPWLDEALTEYSTIVYFEQKYGKDMAN
                     KLIKTMEIQTKSYLSENIFKPANEYKNSTEYSLNVYTKGAIAFNEIRKEVGDKVFFET
                     LNEYYNKYKHKNANGRAFVELWNSKGVDINKIISECK"
     gene            39555..40352
                     /locus_tag="ACGI59_000520"
     CDS             39555..40352
                     /locus_tag="ACGI59_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFG9738329.1"
                     /translation="MLKYCSVGSGSSGNCHYIGYKNTNILVDAGLSGKRITTGLKDIG
                     VDADKLKGIFITHEHVDHIKGAGILSRKFDIPVFANIKTWCSMKDKLGDIKDKNMKVF
                     ENDKTYSLGDIIVRPFSIEHDASDPVGYNFYTESDEKMSIATDIGCITQNIKKHLYKS
                     KLVVLESNYDPNMLMMGSYTYALKKRVMSSTGHLSNEDAANFCVELINEGTESILLAH
                     LSKENNFPELAYETSKGVLASNDIVVGQDVKLDVLSRNDVSNVYEMK"
     gene            40365..42467
                     /locus_tag="ACGI59_000521"
     CDS             40365..42467
                     /locus_tag="ACGI59_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFG9738330.1"
                     /translation="MLKRNFENVEDNKILYSIKQGMILAIPAIMTGSTALVILNLPIK
                     AYQEYLSGLFNGEVTNILNFINDSTLGIISLIILLTISYSYGKIYGSKYTVLVPIVAM
                     CSFLVFSHGNELNSYIEIFKTKWLFTSIIVSMTSSVLFVKLTESFVTSVKFHTEGADA
                     DFNMVVSAIVPFIIVVFLFSIFRVVMISLIGSSNFQDIFYNLFSNVFNKMGTNLMSAL
                     LFIFLMHFMWFFGIHGSNVLDTVAKNLFENSMAININLVNNNQLPTEIFTKTFFDTMV
                     LLGGCGSLLCLVIAIFLSEKRINVRKLAKIASIPALFNINEMLVFGIPIVFNYIMFVP
                     FVITPIILTVTSYVAMSTGIVPCTVSAVEWTSPIFLSGYMSTGSIRGSLLQVFNLCVG
                     VMIYIPFIKMSQNRYTYMFKNNIENLTNLLKRYELTGEQPNLLNHNGKIGSISKMLAS
                     DLKFAMKRGEIELFYQPQVNYNGNVVGAEGLLRWKHSIGGFIYPPLVIILAKEEGFLD
                     ELGEYIINKACIDLKKSEKLLRNPVKFSVNISPDQLDDPKLPEQIKNIISSNDINPNM
                     LGIEITEQIALSGSQIIIERINAIHNLGIKLIMDDFGMGHSSLIYLQNNNFDIVKLDG
                     SLVKEILTNKRSSEIIMTIVNLSKNLDFDIVVEYVENIDQRDKLYGLGCEIYQGYYYS
                     KAIPFEEFVEYANQEREI"
     gene            42535..43014
                     /gene="rlmH"
                     /locus_tag="ACGI59_000522"
     CDS             42535..43014
                     /gene="rlmH"
                     /locus_tag="ACGI59_000522"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFG9738331.1"
                     /translation="MNISIVVVGKIKEKYLKLGIDEFKKRLSKYCKLEIIELDDEKAP
                     ENLSVKEMEIIKDKEGKKILGKIKHNSYVIALAIDGKGLSSEELAKTMSDLAVRGNSS
                     LCFIIGGSLGLSDEVLGRADYRLSFSKMTFPHQMMRLILLEQIYRAYRINNGEPYHK"
     gene            43163..43474
                     /locus_tag="ACGI59_000523"
     CDS             43163..43474
                     /locus_tag="ACGI59_000523"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFG9738332.1"
                     /translation="MIKIMLACSAGMSTSLLVTKMESAAKENGIESQIWAIPESTIQN
                     EIEKCDVLLLGPQVRYVLPKAQEIAKPYNIPVEVINMMHYGTVNGEAVLNRAIELNNT
                     K"
     gene            43522..43839
                     /locus_tag="ACGI59_000524"
     CDS             43522..43839
                     /locus_tag="ACGI59_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFG9738333.1"
                     /translation="MDEKTIEISFNIIGYAGEGKGLAFEAIKEAKNGNIEKAKELLKE
                     SREVINKAHRYQTELIQNEASGNRTEISVILIHAQDHLMNGMNFQQLAEEIVDLHLKL
                     QDK"
     gene            44052..44753
                     /locus_tag="ACGI59_000525"
     CDS             44052..44753
                     /locus_tag="ACGI59_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFG9738334.1"
                     /translation="MLRNQPLYIQVVDILKQKILNNEYKIDENIPTERELEQEFGVSK
                     ITIRKAIEILESEGYVEKKSGKGTKVISNSLFNKLSKAESFSSILLSKGHKLSKETIS
                     IEKIINDTNSQVFSIFGEKCSKITRMYYLDSKPYIFFTHYIPFDENLTVESFNNESSL
                     YMYLYRRNINITRFEDEFFIEESELEVSKALNLAKDILLGRKRKTYDENDNIVELSFA
                     RYNTEVQNYVIKYEI"
     gene            44977..46278
                     /locus_tag="ACGI59_000526"
     CDS             44977..46278
                     /locus_tag="ACGI59_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFG9738335.1"
                     /translation="MEKFASFLERKIMPVAAKISNQRHMRAVRAGIIATLPLTIVGSF
                     FTILLNIPIDAYMDFIAPFKAILDIPFRYTVGFLSLYATFGIASSLAKSYDLDTTGVG
                     MLAVMAFLVSTIVPTQVVEPVGGVIEAGRWMDMAKLNSPSLFGAIVTSLITVEIYRFM
                     KEKNITIKMPAGVPVEVSNSFTALLPTAVILMLFWVIRHILGFDISVALSAMLSPLKG
                     FLSGNSLGGGLLTVFLITLFWVLGIHGSAIMGPVIRPIWDMSIAENITAFTEGVSAHA
                     MPNIFTEQFLQWYVWIGGAGATLALVVMFMFSKSQYLKSLGRLGFLPGLFNINEPIIF
                     GAPIVMNPILGIPFIIGPIIMTILSYVLTITGVIPMMVARLPFAMPAPIAAVMSTNWS
                     ILAGILVLINFVISFAVYYPFFKVFEKQQLQREQEEMSENL"
     gene            46415..47509
                     /locus_tag="ACGI59_000527"
     CDS             46415..47509
                     /locus_tag="ACGI59_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFG9738336.1"
                     /translation="MERRLGISVYPEHSNMEDDKAYIKMASKYGFKRIFMCMLSITRP
                     KEEVKKHFKEIITYARELGFEVILDIAPNIFDILQISYDDLTFFSELNASGIRLDYGY
                     DGSKEAMISFNPYDLKVELNMSNDVAYLDNIITYKPNVNNIYGCHNFYPQRGTGLPYD
                     FFVRCSNRFKKYGIRTAAFVNSKYATIGPWNINDGLCTLEMHRNLDIATATKHLFATG
                     LIDDVIVGNAYASEDELKRMSEVNKYQVVFNVVTEEKTSEVEKTIMANEQHFRRGDIT
                     NQVARSTEVRKKYAKENFPKHDNTTIFKRGDVVIGNDDFGKYKGELQLILQDCADERK
                     NKVGRIKEDEIILLDFIEPWSKFKLILEGE"
     gene            47511..48644
                     /locus_tag="ACGI59_000528"
     CDS             47511..48644
                     /locus_tag="ACGI59_000528"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HFG9738337.1"
                     /translation="MKIDILIKCGKTVDKSIIDIAILDDKIIEVKNQIDDSKYQANQI
                     INLDGEKFISAGWIDIHTHCYERLDLYKDYPDEVGIKSGVTTVVDAGTTGALDIGKFY
                     EDTKKYKTNVYALINIAKQGITSQDELSSMMNIDEYELRRAVKKYKDFVVGIKARMSK
                     SVVISNDVEPLKVAKRIKNELNLPMMVHFGSSPPTIEDIFDYMEKGDILTHIYNGKPN
                     GILRGNEVKKEIIEARERGIILDVGHGTESFSMDIAMKSKDAGIFPDTISTDIYIKNR
                     INGPVYNLSTTMEKFIYMGYSLEDIIDKVTKNAADAISLKNKGLIKEGYDADLTIFDV
                     VNEEKELQDSLNKSVITSTSIKPRAVVVNGEYLNIGKSIGENE"
     gene            48662..49771
                     /locus_tag="ACGI59_000529"
     CDS             48662..49771
                     /locus_tag="ACGI59_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgaE family pyridoxal phosphate-dependent
                     ammonia lyase"
                     /protein_id="HFG9738338.1"
                     /translation="MSSIFEKYSVRRVINASGKMTILGVSTVDKEVIEAIGEGCKNFF
                     IIEELINKTGEYIANLLGCESALIVSSASAGIAQSVGAMIAKDDMSLVYNVNNPKKQV
                     KREIIIPKGHNVDYGVPVEVMIRLGGGEVVEAGYANMCNKEHIIREINENTAAIMYIK
                     SHHCVQKSMLSVLDAVQVAKEYNLPLIVDAAAEEDLNVYYELGADLVIYSGAKAIEGP
                     SSGLVIGKKKYIDNVKLQSKGIGRAMKIGKENIVGLTCAIERYISNDKVTLKEMEDKL
                     NPFIDKINTIKGVSASITRDSAGREILRGEIDFNEDIINKSTQQIINELRSGEIAIYT
                     RDYKANEGKIEIDIRSVTGSDLDTIYNRIKNIVES"
     gene            49836..50576
                     /locus_tag="ACGI59_000530"
     CDS             49836..50576
                     /locus_tag="ACGI59_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KDGP aldolase family protein"
                     /protein_id="HFG9738339.1"
                     /translation="MNCIPQYYKNRVCLNVLAGSIKNAKEVYDACDGHVLIGILSKNY
                     STVEAAIEDMKKYSKEIDNAISVGLGAGDPNQSNMVSQISKELQPQHINQVFTGVATS
                     RALLGQNDSIINGLVSPTGKPGFVKISTGPLSSKEKDGIVPIETAIAMMKDMGGSSIK
                     FFPMGGLKTKDEYIEVAKACAKHNFYLEPTGGIDLDNFKEIVQIALDAGVEKVIPHVY
                     TSIIDKETGETKVEDIGVLYKIMKELLG"
     gene            50581..51573
                     /locus_tag="ACGI59_000531"
     CDS             50581..51573
                     /locus_tag="ACGI59_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFG9738340.1"
                     /translation="MNILAFGEIMMRLSVPDYKFLTQTNELNYITTGTGLNILSGLKN
                     FGYNTYMLTKLPNNNVGKASSANIRKLGVKDDFITYGGNHIGVYFLENGYGERPSEVT
                     YLNRLNSSFCESKLQDYDIDKCLEEMDIVHICGIAMQLTDSVKEIALELARKSYEKGI
                     KVFFDFNFRQSLNPERTYDDLVNDYKKILPYCHGLFASYRDINKILNIDSLNIKSSSE
                     LIKKEYDIDIVAGTMRRFDDMNNRFIKGYISNDDGYFESREYKLEIYDRVGAGDGYVA
                     GIIYSYLENMKSEDIVEFGVASSVLAHTTYGDNSLVSTGHVLRLMNNEKIDILR"
     gene            complement(51761..52642)
                     /locus_tag="ACGI59_000532"
     CDS             complement(51761..52642)
                     /locus_tag="ACGI59_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFG9738341.1"
                     /translation="MNTKQLEYFISVAENLSFTKTAEKFYISQTAVTQQIKALEEQIN
                     VTLFTRSKRHVELTPAGKVFLSEARTIIKNINDAIAKTQQFAHGFFGTLSIGTLIGYE
                     KNKLQQYLKEFSNTYPNISMDICTNEITELLNLVKNNSMDLAFVINPENQPLKDFEYK
                     TVERYSLVALLPPGHPLYNEDSIDLIELQHDKFIFVKETGDEYGQKSMIQNRYREAGF
                     VPNVVQRSNNLNTIESLVSSNMGISILPSFCVTDTMIERDIAIVPIKEVENRIEVVVV
                     WNKKNTNPALEKFISII"
BASE COUNT        20027 a         6467 c         8613 g        17646 t
ORIGIN      
        1 ttcctttttt caaggacaag taatactata ccatctttaa aacagtaggt caatactttt
       61 tttatatttt tttataaatt tttttcatat tcttttttca gcttctctac tacctttttc
      121 tctagtctag aaacagtcat ctgtgatatc cctagttctt tagctatact agattgtgtt
      181 ttatcaaaga aaaatctatc tttaaaaatt ttaacttcta attcatttaa agtttcaata
      241 aacttattaa taaaatcttc atactctata cttccaaagt taccttcttc ttttccaatc
      301 ttatcaataa gagttatatc tttatcctct gaatcatcat tacttgatga atccaaagac
      361 attggttgat atccataaga agcctccata gcctctaaaa catcttcttc actaacgcct
      421 atataatcag ctatatcttt tacctttgga tgcttcttat tttcttgttc taatttaatc
      481 ttcgcatcac ttatcttttt gcttaattcc tgtattcttc ttgggactct aagtgtccat
      541 accttatctc taaaatactt ttttatttct cctactatag ttggtgttgc aaagctggag
      601 aactcaaatc ctttctcaac atcatatcta tcaatagcat aaattaatgc caatgaagca
      661 acttgatata tatcttcaaa atcaacacct ttgtttatat acttcttagc taacaatctt
      721 gctaaataca gatgtctttc aataagaata tttctaatgt ctacattttt atttttatta
      781 tataaattaa atagctcttt tgtatccata tttaggtaat gtgtagcatt agctacattt
      841 ttcattaaat atcaactcct aaatatttag tcatttttat ttttgtccct tgattatgtt
      901 ctgattcaat atctacatca tccattaaag ttttaattat aaacaatcca agaccactct
      961 cttttggatt ctctaaatct ggtgtactta ttgagctaac atcatatcct tttccattat
     1021 cttgaatttc tatattaatt gcattatcta aaatattaaa tactatatta aacacatcat
     1081 ctttactgtg ttttattgca tttgtacatg cttcagatac tgctaccttt atatcttcta
     1141 tatcatctat tggaaaaccc atcttattgg caattccaga tgctgttaat cttattatac
     1201 taacataatc tggatttgta gtaatttcca tctttatagt ctcacaagcc aaagtttatc
     1261 cctccacttt aaatatttta tctagacctg ttatagtaaa aatctttctt acgttactct
     1321 taggatttac tatgtatatc tctttttcat ttatttttaa tttttttaat acacctatca
     1381 tagctcctaa gcctgttgag tctatataat ctaaatcttt aagattaatc tttacatcta
     1441 acatattttt ttctattaaa gcatgtaaat gttcctttag tttatctgct gtagaaacat
     1501 caagttctcc atcaagacaa acattccaaa atttattttg tgaatctaaa tttgaatcaa
     1561 tattcataga catgtaaaaa cctcctattt ccttttaata attttaaata tatttcctat
     1621 actcaatatt ttattaatta cataaactat atcatctatt ccctttgata ttgatgatgc
     1681 attttcaaca atatcttgta tatgtctttc attataatct acaatcttat atgccttttc
     1741 aacaacctta tttatgctgt ttagagtttt agctaaataa attgctacta taagagcaga
     1801 gcttcctact aacactgctc caatttgcca cccccatgca ttcattagtt aacatctccc
     1861 tcatcatcaa agcttttact tattactata tcttcttctg aaatctttat atcttcttca
     1921 ttattatcat catccactaa atttattatt ctctctttaa catcattaaa tgtttcatgt
     1981 agaacttctt ttggattttc tttgacatca ttaaacttct cttttgtttc ttttcttagt
     2041 tcagaacctt ttttaggtgc aaaaaacata ccaaatataa accctaaaat agctcctaat
     2101 aataaaaatc cacctttttt acaacttctt ttattgcaca taacaattac tcctttcaat
     2161 tttaaatttt tatctaacta cattatacaa aaagaatagg atatttatcc tattcttctt
     2221 caatatttat tttggcaatc gaaactacat tgacttcatc attcgtcttc attaatttaa
     2281 caccacttgt aactcttcca aacagtgata tttcattgac tcttattcta ataagaactc
     2341 catcagaatt tataatcatt atctcatcat cttcgttgac catatctgca cctacaattg
     2401 taccagtttt ttcagatata ttataagttt taattccttt accagctctt atttgacttc
     2461 tatactcttc tatatttgtt ctctttccaa aaccattctt acttacaact aacacatctg
     2521 tacctttact gcaaaggttc atagacacaa caaaatcttc tttacttaaa gttatacctt
     2581 ttacacccat tgctgttcta cccatatatc ttatatcatt ttcatcaaat cttatagaca
     2641 taccttcttt agttactaaa agtacttctt gttttccatc tgtaagttct actccaataa
     2701 gctcatcatc atctcttaaa cctattgcaa taagaccaga tttatttata tttttgaact
     2761 cttctctctt agtcttttta acaatacctt ttttcgttgc aagtaataaa tattcatttt
     2821 catcattacc atcaataggt attagagtag caattttctc atctgctgac aattgaagta
     2881 aattcactat agcagtacct ttagcctgtc tcttaccttc tggtatttca tatgcattta
     2941 atttaaatac tctaccttta tttgtaaaga ataatagtct actatgagta gttgtagtta
     3001 ccaagtgcct tacaaaatct tcttctctag ttgtaagtgc tgaaatacct cttccacccc
     3061 ttttttgact cttataagta tcagatggaa gcctttttat atatccaaag tgagtaagag
     3121 ttattgctat ttcttcatca cttataagat ctctcatatc tatttcgcct tcagcatgtc
     3181 ttatttctgt tcttctctca tcagagtaat tttcttttat tattgtaatt tcatccttta
     3241 ttacatttaa aagtaatctt tcatcagcta aaatctcttt taatctattt atcttcttga
     3301 ttaaatcttc atattcagct tctatcttat ctctttctaa acctgtaagt ctttgaagtc
     3361 tcatatctaa tatagcttgt gcttggattt cagttaattt gaatttttct attaaaccta
     3421 gcttagcttc ttgcccagtc tttgaagctc ttatcaagct tataacagca tctatattat
     3481 ctaaagcaat ctttaatcct tctaaaatat gtgctcttgc ttcagcttta tttagttcaa
     3541 atttagttct tcttgtaaca acatcttctt gatgtttaat ataatgatat aatatttgtt
     3601 taagatttaa aactcttggc tgaccatcta caagtgcaag cattattata ctaaaagtat
     3661 cttccatttg agaatgttta tacaaattat ttaatactat attagcatta gcatccctct
     3721 ttaattctat aacaattctc ataccatttc tattactttc gtctcttagg tctgatatac
     3781 cttctattct cttttcttta actaactctg ctattctttc aaccagttta gccttattta
     3841 cttgataagg tatttctgta actattattt gctgttttcc ttttggtagc tcttctataa
     3901 aagctctaga cctaacttta acttttcctc ttccagttct gtatgcttct gctatacttt
     3961 cttttcccat tataattgca gctgtaggga aatctggtcc ttgaacaaat tttattaaat
     4021 catctacact acactctgga ttatctatca aatatacagt tgcatcaatt acttctgcta
     4081 aattatgtgg aggtattgaa gttgccatac caacagctat accatttgag ccatttacta
     4141 ataaattagg atatctagct ggcaatactg aaggctcttt taacgactca tcaaagtttg
     4201 gtttaaagtc tacagtttcc ttttcaatat ctctcagtag ttctaatgat aatttactca
     4261 ttttagcttc tgtataacgc atagcagctg gtgaatcccc atcaacagaa ccaaagttac
     4321 catgaccatc tactaaaagt gctctagttg aaaaatcttg tgccattctt accatagcat
     4381 aataaacagc agtatctcca tgaggatggt acttacctaa aacgtcccca acaatacgag
     4441 ctgacttcct gtatggttta tctggagtta aatttaactc actcattgaa tataatattc
     4501 ttctatgaac tggctttaaa ccatctctaa catcaggaag agcacgtcca gctataacac
     4561 tcattgaata atcaatatac gattttttca tttcttccgc tatttcaata gggagtattt
     4621 tgttattttc ttccatgtat actaatcccc tctctctata tatctaagtt tctaacaaat
     4681 cttgcatttt cttctataaa ttcttttctt ggagcaacct tatcacccat aagcattgaa
     4741 aatacttcat ctgcaattgc agcatcttct acagtaactt gtaataaagt tcttgtctca
     4801 ggattcatag ttgtttccca taattgctct gcattcatct ctccaagacc tttgtacctt
     4861 tgaagttcaa caccatttct tccaatttca tctagtaaga tatttaactc tttatctgag
     4921 taaacataat gttccttctt ttgctttgtc actttatata aaggtggctg tgctgcataa
     4981 acatacccct catctataag aggtctcata tatctaaaga agaatgttaa taataaagtt
     5041 cttatatggg ctccgtctac atcagcatcg gtcataatta taattttatg atacctagcc
     5101 ttatctatat caaaatcttc tccaatacca caaccataag ctgttatcat attttttatt
     5161 tcatctgaag atagtatcct atctagtctt gatttctcaa cattaagtat tttccctctt
     5221 aatggaagta tagcttgact atttctatct ctaccttgtt tagctgaacc tcccgctgaa
     5281 tccccttcga ctaagaatat ttcactttta gatggatctt tttctgcaca atctgcaagt
     5341 tttccaggta aagatgtact ttctaataca ctttttctcc ttgttaattc tcttgctttt
     5401 tttgcagctt ctctagctct ttgtgctctt aaagctttat caactatgat tcttgctgtt
     5461 gatgggtttt cttctaggaa agaccccagt tcatcaactg ttacactatc aactatacct
     5521 ctcataaagc tattacctag ctttgtttta gtttgacctt caaattgagg ttctggtaat
     5581 ttaactgata ctactgctgt aagaccttct cttatgtctt caccaagtag gttaacatca
     5641 ttttccttta aaaatttatt tctcttagca taatcattta cagttttagt aagagctgct
     5701 ttaaatccac ttaaatgaga accaccttca tgtgtattta tattgtttgc aaaagaatat
     5761 atgttctctg tataaccatc tgtatattgc atagctagtt caacaaaaca atcatcaacc
     5821 tttttatcta tataaactat gtcatcatgt ataccagttc tagatttatt taaatactta
     5881 atatattcta ccaaaccacc tgtatagtga aattcttttc ttttttcttg tccttctctt
     5941 ttatcttcaa atactatttt tattccttta ttcaagaaag aaagttctct aagcctatac
     6001 tccaatgttt catatttaaa ctctatttca tcaaatattg tctcatctgg cataaattga
     6061 atcattgttc ctttatcttg agattctcca actacttcaa gcttagatgt tggcaatcct
     6121 ttctcatatg tctgcttata tatctttccg tttaaataaa cctctgctac catccatttt
     6181 gacaatgcat taactacaga aacaccaact ccatgaagac ctccagatac tttatatcct
     6241 ccgcctccaa actttcctcc tgcatgaaga ttagttaata ctgtttctaa agttgattta
     6301 ccagttttag gatgtatttc aacaggtata cctcttccgt tatcttttac taaaacactg
     6361 ccatcttcat taatagatac atatatgtct gaacaatatc cttgtaaagc ctcatctata
     6421 ctattatcta caacttcata aactaagtga tgtaaacctc tagggcttgt actaccaata
     6481 tacatacctg gtctttttct aacggcttct aatccctcta atacttgtat ctgacttgca
     6541 ccgtattctt gtttcattta atttcctcca ttctctatgc taataacatc gccattttca
     6601 atattaaata ttgtaacatt tttttcatca aatatttttt tatgtgaaac ttcagcactt
     6661 gttataaaca tttgaacatt gcttaaatta tttactaaaa gtttttgtct tgcttcatct
     6721 aactcactaa aaacatcatc taaaattaat actggatatt cttcaacttc attttttatt
     6781 aattctatct ctgataactt taatgatatt gaagcagttc tttgttgacc ttgtgaacca
     6841 aaaagtcttg catctaaatc atttatgaat atatttaagt catctttgtg tataccatat
     6901 ctggtagtct tagattctat atcattaggt ctattcgatg acaacttggc taaaaattta
     6961 ttatatactg tatctatagt atcttcatca gttatattta tttgattttt atatcgaatt
     7021 gataatcttt caactccatt agtcaaattc atatgcatat tttcagatat attagctatc
     7081 ttttttataa agtctctacg caaaatataa atataacttc catactttgc taacgtatca
     7141 tcatatacat ctaataaagc ttcatcaaca tgtatatttt taagcaccct actcctttga
     7201 gaaagtgttt tattataatt ggtaagatat ttataatatt taggtataat ctgactaatc
     7261 tctttgtcta taaatgccct tctttcttta ggaccctctt taaccaacct taaatcctca
     7321 ggagaaaata taacaacatt taagttgcca agtagttctt gtatcttttg taaaggaact
     7381 ttatttattc ttatcccttt tttatctttt ccaataatta gctctattag actatattta
     7441 ttatattttg aaaaacttcc tccaatatat aagttttcac tattaaatct aaccatttct
     7501 ttatctttgt tagtcctaaa tgattttcca aaagaaagca tataaataga ttctactata
     7561 tttgtctttc cttgaccatt tttaccaaca agtagattta ctttcccatt aaattccaga
     7621 tgcaactttt tataatttct aaaattaaca agttgcaaac ttttaagttt cacaaaaagt
     7681 tctccttttt ttacaatact tttattttag ttccttctat ttcaactata tctcctgatt
     7741 ttatcttttt tcctcttctt aattctattt catcatttac tgtcactaaa ccttcttgaa
     7801 ttaaaaattt tgcatgacct cctgttgatg caatctctgc taatttaaga aattgatcca
     7861 actttatata ttctgattct atagttattt cagtcataat agttcctcct tgtcactatt
     7921 ttaaacaaat tcctattgat acactaaaag tatattatac cataaatatg cttataaaag
     7981 gagttgtcta agattagaaa aattttctac aaaagacaac tccttcatgt ctgctatata
     8041 tttgatgata ttcttactgg aagcagtaag taaatataat taacatcatc tgttggttta
     8101 attatacaag gatttacatt tgtagtaaat tctataaata tttcctcatt atctatattt
     8161 ttcaaacctt cgataaaata tctagaattg aaagctatat ctaggtaatc tccatctaaa
     8221 tctatttcaa cttcttcata cacatttcct ttttctgtat tagaagtaat tgccattact
     8281 ttatctctta tagataactt tataagatta tttttttctg attgagacaa taaagacgct
     8341 ctctctatac tatttaaaag ttccttagta tttaatttaa ctctgctgtt atgttctctt
     8401 ggcaataatt ttttataatc aataaaatct ccttcaagca atcttgtaat aattttcgta
     8461 tcattaatta taaaaatagc atttttttca ttaaatccaa ctttaacatt atcctctcca
     8521 gaaagcaaac tatttacatc tataagtgtc tttccaggaa ttatgacttt tatattttca
     8581 gttaaactat caaccgaaca actttttaca gctaatctat atccatcaat tgctactaaa
     8641 tttaagtttc tatctacaat ttctaaaagt tctcccatta aaacaggttt tgtttggtcc
     8701 tgtgagatag caaatacagt ttgtttaatc atatttttta ggatttcttg aggtatgcta
     8761 tataaatctt cttcatttaa ttctggtaat ttaggaaatt cttttgctgc ataaccttta
     8821 attttaaatc ttgaatttac acaatttatg taaatattat tttctgaatc agtttctatc
     8881 tcaacaaagg aatctggtaa ttttctaatt atatctccaa atagccttgc atctacaaca
     8941 acatcacctt tttctatgat ttctgcttgt acataagttt ctattcctat ttcagcatca
     9001 tatccagtaa gttttaattg accttcttct gtagatataa gtataccttt taataattct
     9061 attgttgttt ttccattaat agctttttga gcaattccaa ttctatttgc taggattttt
     9121 tgattacaaa ttattttcaa tgtggataac ctcctctggc tttttatata taaaaataag
     9181 ttaaaatcat agtagtaata gtagtaggtg ttgttgataa tgttaataag tatgtataac
     9241 attataaata caaagatatt aacatgtgta taagatgtta atatctttgt tattttagac
     9301 ttgataattt gttaataatt tttgttcaaa aaaagtatta acaggttatt caagagatat
     9361 aaacatacaa ttgtgtataa attatccctt caaatctgat ataattttgt ctattttagt
     9421 cttaatttct tcactttctt ctatatcctt agatactttg tcatgagcat gaattactgt
     9481 tgtatggtct ctacccccaa actcttcacc tattttagga agggataaat cagtaagctc
     9541 gcgcgttaaa tacatagcta tctgtcttgg ataagctatt gaacgtgttc tttttttaga
     9601 attaaaatct tccatcttta aattgaatac agaagatact ttttctttta ttctgagaac
     9661 atttatttct tcatttttag aagttgttat aatatctttt aaagcttctg tagcaagatc
     9721 aaaagatatt accctattgc taagagaaga ataagcaacc actctagtta atgcaccttc
     9781 tagttccctt atgtttgact taatattttt tgctatatat gacataacct catttggcac
     9841 atctatacgt tctagttgag ccttttttct aagtatagca attcttgtct caaagtcagg
     9901 tgcttgaata tcagttataa gtcccatttc aaatctcgat cttagcctat cttctagtgt
     9961 aggtatgtct tttggcggtc tatcactaga tataattatt tgtttgtttg cttcatgaag
    10021 agtattgaat gtatggaaaa actcttcttg agttctttct tttcctgcta taaattgaat
    10081 atcgtcgatt agtaatacat ctacgtttct atatttattc ctaaattctt cgtttttatc
    10141 gtcttttata gagtttataa gttcattagt aaatttttct gaagaaacgt aaactacttt
    10201 tgagtctttt ttttgactaa caatatgatg acctatagca tgcattaggt gagtcttacc
    10261 taatccaact cctccataca aaaaaagtgg gttataagct ttagctggag attctgcaac
    10321 tgcaacgcat gcagcatgtg caaatctatt actattacca ataacgaatg tatcaaatgt
    10381 gtattttgga tttagattgg ggtaaagaac tctataattt aaatctgtgc tatcagaatt
    10441 taaatcagca acctcttttt cgctcaatac aaatttaata ttatattttt ttaatgacag
    10501 ttgactaata gcatcttcaa ttaaatgtaa atatctattc tctaagatat ctttattaaa
    10561 atcactagga gccagtaaga ttaaatcatt aagatgtatt tttaatggta cgatgttttt
    10621 aaaaaaggta ttaaaactta ctgaagttaa gtcacctttt attaattgta gggttttgtc
    10681 ccataaagaa actatatcca taatataaac ctccgaacag aataattaat attttccaca
    10741 aagttattaa cagaaaagca aaaatatata taaattttac agaataacta aatttatgga
    10801 taatggtgtt gataaattaa taatagtata aattattaaa aaaatatgaa aagatataaa
    10861 caatatgtag ataaagttat aaataacata aacatacaat aaatggtgat tataactaga
    10921 gtattgtcag taatcttaaa agttatacac aaaagtatcc acaagctgtg aataatttct
    10981 aataagttga taacatttat gcacattatc aacaagtata tttataatat caaaatatat
    11041 gtcaaacttc aatgatttgt aactaattta tccacaatat taactaattg ttgataaaaa
    11101 tataaacatg ttaaaattac tttatttgtg tatataattg aaaaaaataa tttagaataa
    11161 aattaaatag atttttacta gtattttcaa taaaaaaaat acataatata tatatggtag
    11221 attttgtgat ataagcgtcc caaaaactcg ttgacaacag tagtaaaagt atttataatt
    11281 atatgtgtta tgtttacttt gagggtaaag ttattatttt tataaacgga aaataaaatc
    11341 tgaaaggcgg tgcaaaataa tgagtaaaag aacttatcaa ccaaaaaaga gacaaagaag
    11401 taaagaacat ggtttcagaa aaagaatgaa gacgtctaac ggaagaaacg tgttaaagag
    11461 aagaagagct aaaggaagaa atagattaac tcattaatta caaaagggcc gctgcaaagg
    11521 tggccttttt gtgtaaataa ttacaattca tagctgtgtt tatggaggtt aactatggac
    11581 tttaatagga ctaaagggtt gaaaaaagac tctgatttta gaaaagtata taaacacggc
    11641 aaatcttttg caaacaaata tttagtaata tatatactta aaaataaatc agattatagt
    11701 agagtaggta tttctgtttc aaaaaaagta ggaaaagcta ttactaggaa tagagtaaga
    11761 agattaataa aagaagccta cagattaaat attgatgaaa aaattaagcc tggatatgat
    11821 atagtattta tagcaagggt atctagtaag gatgctactt ttaaagacat agacaagtct
    11881 ataaaaaatt tagttaaaag aacggatatt tctatttaat tgtatgctat aaatatggag
    11941 gttaatgact ttgaataaaa taatttatat attaaaggaa ataagtaagt atttatcgaa
    12001 tttatgtatt tatttagtga gattttatca aaagtatata tctcccttaa agggacctac
    12061 ctgtagattt tatccaactt gctcacaata ttcaatagag gcgtttaaga aatatggatt
    12121 tgtaaaaggt atgtatttaa cgataagaag aattttaaaa tgtcatcctt ttcatccagg
    12181 aggttacgac cctctaaaat aaaagagagt aactattagg aggtataaaa aaattgaata
    12241 taataggaaa tttcttagga ttgattctta aggtaatatt tgaatttgtt aatcattatg
    12301 gtatttctat aatacttttt acgatacttg taaagattat tttattacca ttaacaatta
    12361 agcagactaa gtcaacaaag gcaatgcaag atatacaacc tcgtataaaa gagatacaag
    12421 aaaaatataa aaataagcca gaaaaacaaa atgaagaaat tgttaagtta tatggagaag
    12481 ctaaaattaa tccactatct ggttgtttac ctttattaat acagtttcct atattaatag
    12541 gattatttag cgttttgaga gaaccagttg ctcatggcgt ttttgcaaac aaagctgcat
    12601 ttttagcagc tgacaatggg tttttatgga taaaaagctt aacatcacca gattatgtat
    12661 tagcagtatt ttcaggagca agtgcatatg tcatgcaaaa agtaatgaca ccaaaagatc
    12721 agttacaagg ctctatgaaa gtaatgacat atgtaatggc aggtatgtct ttctattggg
    12781 gatttatatt cccagcagga cttacattgt actggacagt aagtaactta ttctcaatag
    12841 ctcaatatta cttaataatg aatcctttaa aagctaaact ggctgctaac tctaaggagg
    12901 aaaaagttaa tgagaaaaag cctaaaatta ataaaaagta agagtaaaga ggaagctata
    12961 agtaaagcta tagcagagtt gaatttaaaa gcagaggata tagaagtaga aatacttgaa
    13021 aatccaagta aagggttttt aggttttata ggtgctaaag atggtactta tgaaattttt
    13081 gttatcgaga gggaagtaga tgtagctaaa aattttatag aggtaatgtt gaaaaatgca
    13141 aatgttgatg ccaaagtcaa tgtatctcaa aaagataatt taatcatggt tgatattgaa
    13201 ggcaaagaag ctgcaagttt aattggtaga agaggggaaa ctttagattc tattcaattt
    13261 ttgactggat tagctcttaa taaaataaat aaagattctc atacaagagt acttgtagat
    13321 atagaaaatt atagatcaaa acgagaagaa tctttaataa gatatgctaa taaagttgct
    13381 agagaagtag cgaaaacaag aaaaactaaa aaattagatt atatgaatcc atatgagaga
    13441 agaataatac attcagctct tcaaaatgac aaatatgtaa ttacatatag tgaaggaacc
    13501 gatccatata gaagattagt aattgagtgt aaaagataaa gtcaacctcc taaacattag
    13561 ggggttttat tttttaatat tattaaatca ttaatgattg gttaaatgta aatctatatg
    13621 taaaaaatta tgaattaaga ggtgataaat ttgtttatag atgatacaat tgcagcaata
    13681 gctacagcac ctggagaagg tggtataggt atacttagaa taagtggaga aaaagctttg
    13741 aaggttgcag aagaaatctt taaatctatg tctggaaaga gtattgaaga atacaataag
    13801 agaacattaa tatatggaaa tatagtggat aatgaaaata taattgatga agttttattg
    13861 gcatatatga aaggaccaaa ttcctataca ggagaagatg ttatagaaat taattgccat
    13921 ggtggattta tttcggtaaa gaagatatta gaattaatac tatcaaaaga tgttagatta
    13981 gcagaggctg gtgagtttac taaaagagca ttcttaaatg gaagaataga tttatctcaa
    14041 gcagaggctg taattgatgt tataaaagcc aaaactgata tagctcatga agttgctcaa
    14101 aatcaattag aaggttcttt atcaaagaag ataagagaat tgagagataa agtcactgag
    14161 atattagctc atgtagaagt tgctatagat tatcctgagg aagatattga gcatataaca
    14221 tatcaaactc ttaaagaaaa aactgatgaa cttaaaaaag acattaaaaa attatatgat
    14281 acagctgaaa gtggaaaaat acttagagaa ggcttaaaga cggtaattgt tgggaaacca
    14341 aatgtaggta aatcttcact acttaattct attttaggag agaatagagc aatagttact
    14401 gatataccag gaactactag agatgttata gaagagtttg taaacataaa aggtatacct
    14461 ttaaaaattg ttgacacagc aggtataaga gatactgatg atattgttga aaaaataggt
    14521 gttgaaaagt ctaaagaatc atttactagt gcagatttga tagtaatggt tttagatgca
    14581 tctagaaagt tgtcagaaga agatatagag atattagaaa aacttaagga taaacaaaca
    14641 atagtgctgt taaataagaa tgatttgaaa caagagattg aagaagaaaa aatacttaaa
    14701 tatgtagaaa ataatagtat aataaaaata tcagctttac aacaagaagg aatagaagaa
    14761 ttgcaagata aaatagagtc tatggtatat aaaggtagta tcaaaaataa ttctagttta
    14821 gtagtaacaa attcaagaca taaagatgca cttagtaaag catataaatc tgcaacagat
    14881 gctttaattg ctttggaaca aagtatgcca tttgactttg tagaagtcga tttaaaaaat
    14941 atatgggact atcttggata tattaatggt gatacagtaa cagaggattt actagataat
    15001 atattccata atttctgtat tggtaagtaa tgtacaaaag taaacttgtt tttattatag
    15061 agatttttaa aaaatttgaa gtgtttctca tgaaacatta attttgaaaa gaaaggaatt
    15121 ataaatgata aagtttgaag caggaaaata tgatgttata gtagtcggag caggacatgc
    15181 tggatgtgaa gctgctttgg ctactgcaag aatgggatat aaaactttga taataacaat
    15241 gtctttggat tccatagcat taatgccttg taatccatca attggaggga ctggtaaagg
    15301 tcaattagtt aaagaaatag atgccttagg tggtcagatg ggattaaata tagacaagac
    15361 atatatacaa agtagaatgt taaatactgc caaaggtcca gcggtacact ctttaagagc
    15421 acaagcagat aaatttaaat atcatgaaga aatgaaaaag acattagaag atgaaccaaa
    15481 tcttgatata gctatggatg aagttgtaga aatacttcat gaaggaaatg ttgtaatagg
    15541 agttggaact aaactaggat gttcatttaa atctaaagct gtaatattgg ccactggagt
    15601 ttatctgaac tcaaagatat atatgggtga agttgcattt tatgaaggtc ctaatgcatt
    15661 ggggtatgca aaatatctta cagatagctt agttgagctt ggccttagaa tgagaagatt
    15721 taagacggga actccagcaa gagttcatag agatagtata gacttctctg taatgtcttt
    15781 acaagagggt gatgaaaaag taactccatt ctcatttatg aatgaaaata tagaaaaaaa
    15841 acaagaacct tgttatttaa caagaactac agaagaaact caaaaagtta tactggataa
    15901 tttaaaaagg tctgctatgt atagtggagt aatagaaagt actggtccaa gatactgtcc
    15961 ttctatagaa gataaagtag taagatttag tgataaaaca tcacatcaat tatttataga
    16021 accagaaggg ttaaatacta aagaaatgta tatacaagga atttcaacta gtttaccttt
    16081 tgaagttcaa ttagatatgt acaaaactat aaagggactt gaaaattgta agataatgag
    16141 acctgcatat gctattgaat atgattgtgt tgacccaact caactaaaaa taagtttgga
    16201 aataaagggt gttgagaact tatttagtgc agggcaattt aatggaactt ctggatacga
    16261 ggaagcagca gctcaagggc ttatggctgg tataaatgca gttagaaaga tagaaggaaa
    16321 agagccattt gtattagacc gttcagaagc ttatataggt gtcttattag atgaccttgt
    16381 tactaaagga actaacgagc cttacagaat gatgacatca agagcagaat atcgactata
    16441 tttaagacaa gataatgcag atatgagatt aactcaaaaa ggttatgata taggattagt
    16501 taaaaaagat agatatgaaa ggttcctaaa taaaaaggct gctgtagaga aagaatttga
    16561 aagactaaaa aatgaaagag ttacaccaaa agaagtaaat agtttgttag aagaaaaagg
    16621 agcaacacca ataaaggttg gaatatctct atatgaattt ttaaagagac cagaggttac
    16681 ttacgaattg cttgaagaat tagggaaagg cgctggagaa gatgtttcaa gagaagttaa
    16741 agaacagtgt gtaataataa ctaaatatga aggttatata gagaaacaat tgaaacaaat
    16801 agaccaattt aaaaaacttg agaataaaaa gctggatgag aaaataaatt attcatctat
    16861 agaaggtctt agattagagg ctagacagaa actagatgat ataaaaccta tttcaatagg
    16921 gcaagcatct cgtatatctg gtgtttctcc agctgatata tcagttttac ttatatacct
    16981 agaacaaatc agaagaacta gaggtggcaa aggtgaataa tatagaactt ctgaaaaacg
    17041 ggattgaagg ttttaatata gatacgaatg acagtatgtt agaaaaattt aaaatctata
    17101 gagagatatt agttgactgg aataaaaaga tgaatcttac tggaatagaa gatgaaaaag
    17161 aagtgtatat aaaacatttt ttagactcta tttcagctgt aaagaatgga tatataaaaa
    17221 atgggatgtc tataatagat gttggtacag gtgctggttt tcctggaata ccattgaaaa
    17281 tatgtttaga tagcttagaa cttactttgt tagattcgtt aaataaaaga ataaactttt
    17341 tagaggaagt tagtagagta ctagagttag ataatattac atttatacat ggaagagctg
    17401 aagattttgg taaagatgaa gactataggg aaaaatatga tgttgctacg gcaagagctg
    17461 ttgctggatt accgattctt atggaatttt gtgtaccatt tgtaaaagtt ggagggtatt
    17521 ttatttgctt aaaaggtccg aatgctaatt tagagctaga agaatcacaa aaggctattg
    17581 atgttttagg attggagtat attgaaaaaa tagatgtaga gttaccagag gtagatttaa
    17641 atcataatat attagttttt aaaaaagtga tggaaacacc agttaagtac cctagaaaag
    17701 ctggtaaacc agccaaaaat cctattaagt aatgaaaaat ttattattgg taagcttaat
    17761 tatttctcag gaaataatta agcttactgt aaaaaaacaa aaataaagga attattaaag
    17821 tagatgcaga aataaatttg tatgtacata tagagaacaa aaataattta tatctaaaat
    17881 aagagaggta tgttatggaa gataaaagag ttatggagat acctatagaa gatgtagttc
    17941 caaatccata tcaaccaaga aaaatatttt cacaagtttc attggaggaa ttgagcaatt
    18001 ctataaaggt atatggtata attcaaccta taacagttag agcaaaagat ggcaaatatg
    18061 aactaattgc aggagaaaga agattaagag cagctaaatt agcagagctt aaaaccatac
    18121 cagcaattat aaataatatg aacgatgaat cttctgctgt tctagcgtta ttagaaaatt
    18181 tgcaacgaga agacttaaat ttcattgaag aagcaattgg ttatgaaaac ttaataaaag
    18241 aacatgcttt tactcaacag caattagcag aaaaattggg caaaaatcaa tctactgtag
    18301 caaataagct tagaatatta aagcttccaa acgatataaa aatgaagcta atagagaata
    18361 atttaacaga aagacatgct agagcatttc ttaaactacc aagtgaagat ttaatgcaaa
    18421 gtgtgttaga caaagtcata aaaaatgaat tgactgttaa aaaaacagaa aaattgatac
    18481 aagatgtatt agaagaaact aaagttcaag aagagcctga taaaaaacag aatataaagg
    18541 gtgctatgag tataagaatt tacataaata ctataaagca ggcctttgat gctatatcaa
    18601 atacaggaat tgatgctaag tataatgaaa tagataaggg agattatatg gaagttgttg
    18661 taaaaatccc taaaaaatag agattactgc ttaccagtaa tctttttttg taatataagc
    18721 cttgtaatat aaattgattt atagacatta tttatactta aaaaacacct attcaacatt
    18781 taaaattaat ttaattctta aaggggggaa catcatgggc aaagttatag ctgtattcaa
    18841 tcaaaagggt ggagtcggga aaactacaac aaatgtaaat ttaagtgcta gcttaggaac
    18901 tttgggaaag aaaatattag tattagattt agacccacaa ggaaatacaa ctagtggata
    18961 tggtattaat aaaaacgaag tagaaaatac aatctatgaa attatgttag atgggttaca
    19021 tattaaagaa gcaataataa gtactgaatt tgagaatgta gatgtagttc catcagctac
    19081 tgaactatca ggtgcagaga tagagcttac atctaaaact aatagagagt atattttaaa
    19141 aaattccata aaagcggtaa tagatgaata tgattatata tttttagatt gtccaccctc
    19201 actggggatg cttacaataa attgtttaac tgctgtagat agtgttttaa ttccaataca
    19261 atgtgaatat tatgctttag aaggtgttag tcaattaatg gagactataa aattagtaaa
    19321 atcaagatta aatgctgata tagaaattca aggtgttgtt ttaagtatgt ttgatggaag
    19381 agctaattta tcaatacaag tagttgaaga agttaagaaa tactttaaag gtagtgtata
    19441 tacaacttta attccaagaa atgtaagact tgcagaagct ccaagtcatg gaaaacctgt
    19501 aatttattat gataaaagat gtagagggtc tgtagcttat ttggagctag cagaagaatt
    19561 tatagattta gaagaggagg aatggtagta tggagaataa gtctaaaaga agtaatagat
    19621 taggtagagg tcttagtgca ttgataccag aaattaaagg agaaacttca gaaaaagaaa
    19681 tagtcaacat agatatagac aaaatatatc caaacgaagt tcaacctaga aagcaatttg
    19741 atgaagaaaa gataaaagta ttgtcggatt ctataaaaaa ttatggtgtt ttacagccaa
    19801 tagtggttaa aatggatgaa aataataaat atatgataat agctggagaa agaagattta
    19861 gagcatcaaa acttgcaaat aaaaatcaaa tacctgctat aattaaagat atagatatga
    19921 aagatataat ggagatagcc ttaattgaaa acttacagag ggaagattta aattcaatag
    19981 aagaggctct agcatataag agtttaatag agcattacaa tgtaactcag gaagaaatat
    20041 cagaagctgt tgggaagagt agacctcata taactaatac actaagactt ttaaatcttg
    20101 gacaagatgt tatagaaatg atagacagtg gtagaatcac agcaggccat ggtaaggctt
    20161 tattgagaat tgcagataaa gatttgcaac ttcaaatagc gaaaaaaata gaagaagaag
    20221 aactttctgt aagagaagtc gaaaatatag ctaaaaaaat atctgaaaat aaacaagaag
    20281 aacctaaaaa atctaaacct aaggatgtat ttattttaga tgttgaagat aaacttagaa
    20341 atatatttgg aacaaaggtg aatatttcta aaggaaagaa aaaaggtaaa atagagatag
    20401 aatactataa tgatgatgat ttaaatagta ttgtatcaat gcttcttgaa tagaacaaaa
    20461 atataaaaca gagaggaaga ttgttatgat ttatttagat aatgctgcaa ctacatatcc
    20521 aaaaccagaa agagtgtata atgctgtttt agattgtatg aaaaattatt gtgcaaatcc
    20581 aggtagagca ggccataaac ttgctatgag agctgctagg gagatatatg atacaagaga
    20641 gaatatagct aagttgttca atgtaagtaa tcctatgaat atagtattta catctaatgc
    20701 aacagattct ttaaatttag cgataaaagg tgtattacaa gaaggagacc atgtaataac
    20761 aacgtctatg gagcataatt cagtaataag acctataaaa gcattggaaa aaaggggtat
    20821 tgaaaatact gtagtaaaat gcgattatga gggattttta gattatgaag atttagaaaa
    20881 atcaataaag agtaatacta aactgattgt aacaacacat gcgtctaatg tatgtggaac
    20941 tttaatagat ataaaaaaag ttggagaaat agctaaaaaa cataatatac tatttttagt
    21001 agatgcatca caaacagcag gtgtttatga tatagacgta aatgaatgta atatagatat
    21061 gttggcaatg ccaggtcata aatgcttatt tggacctcaa ggaacaggta tattatatgt
    21121 aagagaaggg ttgaacttaa atatattaaa agaaggtgga acaggtagta aatcagaaga
    21181 aatagttcaa ccagagttat tcccagataa atatgaatca ggaactcata atactccagg
    21241 tatagcaggt cttaaccaag gtatactatt tatatttgaa agaggaatca acaatataag
    21301 acaacatgaa gaagaattat gtcaatatat gatagacaaa ttagaggaag taccagatat
    21361 aaagatatat ggaccaaagg acagtaaaaa aagagcatca gtaatagctc taaatatagg
    21421 agatatggat tctggagagg ttactttctt attagatagt gattataata tagcgactag
    21481 atcaggaata cattgttcac cactagcaca taccacttta ggtactttaa aacaaggtgc
    21541 agtaaggttt agtataggat atttcaacac aaaagatgag atagataaag cagtggaagc
    21601 tttgaaaaaa atttcaaaaa ataaatagca tgagatttaa taatatagaa ataaaaaaag
    21661 gggatatttc aaaataaaaa tttgaatcat ctcctttttt gtacaaaaaa gaaaaggggt
    21721 tcctaagaac ctctaatcta tttgataata gtttcaagaa attactttaa gtaatctcct
    21781 tcttcaagaa ctatatgacc actttcgtaa ttagaaccat caacagtaat ttttatttgt
    21841 tcaacattca tattttttat gtaagtacga gcaactgaat caagcattaa agtttctgca
    21901 tcactaccta agtttgactt tgtaaagtca gagctaacat ctaatatacc agtttttaca
    21961 ccatcaatgt cttttgtagt aaatgaattt aattttgcag attctggaac aactttaagt
    22021 ttttgaagtt cttcaaataa agtgttctca tcaattttat ttaaatcaac tttattttca
    22081 attaaatctg tcttatctac atcaaaagag taaatagtag ctgattgagt tttagtttct
    22141 tcttttggag taggtttagc tttgttatta ggaacctctt tttcagtatt gttttcttct
    22201 tttggttgct caacatttgt attgctctta gtttcttttt tagatgaatc acttccattt
    22261 tgagcattag tacaaccaac tgcgaatata gatatagata atatacttaa cactaacatt
    22321 attttcttat ttttcataaa aatttctcct ctcttaaaaa ctattcttat atataattat
    22381 agtcgttatg agcaaatata tgaatacaaa atagtaacaa atttaaaaaa tgatttattt
    22441 aaattaataa aactcaaaat aaactaaatt ttagtcattt ttatgggaaa tcatttaatg
    22501 tacagtgaaa atattgtaaa ataaaagtag tgtttttaaa ataaatataa gttataatta
    22561 tattataaaa aaatgtcttc aaaaggtggg aatagtatgg aaattttgag cttaggagaa
    22621 aagattaaaa aattaagaaa agaaaaaaac atgacattaa aagaattagc tggagatagg
    22681 ataacagcag cacaaataag ccatatagag agggataaat ctcatactag ttatgaactg
    22741 ttggaatatc taggcgataa gctagatgta agtgtagaat acttattaga gaccaaggaa
    22801 atgcaatcaa aaaagataac tgataattta atactacaga gcgaaattta tataaaagaa
    22861 aatgaattaa acaaggctga ggaccaaata aaagaagttt tagatatatg taagagatac
    22921 gatttaatgg agaactatgg cagatgtaat tttttaatgg ctaatattaa tttgaaaaaa
    22981 tctaattata atgatgctat agaaaacttt gaaaaggcat tgtacttctt tataaaaaat
    23041 agtgatagtg aaaatatact taagtgttat ttaaacatag ggaaaatata tatgcaggaa
    23101 gacttttata aaggtgctat aagccacttt aattttgcag aggaaatttt gtcagagaat
    23161 aaatttgagg atgttaacat acacaaggaa ctatatagta agatggcata ttgttacata
    23221 aaactagata atccaaatat gtcattgaag tacatagaaa agataaatga aatagataat
    23281 agaaatgata gaaaagaaga tgtcgatatt ttggtgttaa aagcaaataa tatgcttgag
    23341 attggaaaat atgaagaatc aagagattac tttaaaaaag cactaaaaat acttgagagt
    23401 gaggataata aaactggatt agcaaatgtg tatttaacaa tttgtgatgt ttacagaaaa
    23461 ataggtgaaa cagatagagt acttgaatat tcacaaaagg tatatgacat aaagaaaaat
    23521 gatgaagatg aatacatgat gtcaggatta tttaagataa tagaagcata tatagataaa
    23581 gaagattatg atttagctag aaaatattgt aagatagcct tagcttcatc aataaaaaat
    23641 aaaaataaat tcaatgaata taaagcattg agattctatg ctaatatgta taaaaatcag
    23701 aatgaagtaa ctcttgcaat tgaatatctg attaaatgca taaaaatagt atcagattta
    23761 ggcaacaata gaatattggc caatttatat atagacttag gtcaattata ttcaaatata
    23821 tcaaaagaaa aagagttaga atattatcaa aaaggtgtat ttatgtacaa aaatttagaa
    23881 ataatgtagg gtaaataaac ctataagggt atagaaacaa tctataacaa gcaagataat
    23941 atctatgata aaatataaat tgtatgaata tttttaagga gatgaatcag acaatgagta
    24001 taaaaataga tgcgagaggg cttgcttgtc ctaaaccagt tataaataca aaaagagaat
    24061 tggataacct agaagaaggt gttgtagtta caatagtcga taatgaaaca gctaaggaga
    24121 atatattaaa acttgctaaa tctttaagtt gtgaagcaaa tattgttgac gaaaaagaga
    24181 attttatatc tatagaaatt agaaaaggta aaaatatact agataaacaa aatgttgtag
    24241 ataataaaga aagtgaatta gacaatacat gtatatttat atcttcagat aagatgggac
    24301 ttggaaatga cgaattagga aaagtgttaa taaaaggatt tatatataca ttaacagaat
    24361 ctaaacctta tcctaagtat gtacttttag ttaatggagg agtaaaatta agtgctgaaa
    24421 atgaagaaac catagaaaat ctaaaaatac ttgagaatat gggtgtagaa gttttatctt
    24481 gtggtacttg tttagattat tataatttaa aagacaaatt acaagtaggt acagttacaa
    24541 atatgtatac tatagttgag acattaaaga atgcatcaaa taccatttct atataatgta
    24601 tgtttgttta ataatataaa attaataata aagattaact caaatagata gtcagaaagg
    24661 attaagtaat gaatcaaatg tatatagtgt catttaattc gacacatcat gcgataagat
    24721 cagaaaagtt atttggtgaa aatagtctaa aagtaatggc tcttccaaca ccaagagaaa
    24781 taacagcaag ttgtggaata tcaattaaat tttcttttga agatatggaa aagataaaaa
    24841 ctattttagt tgaaaataat gtagatataa aaggaattta ttgtataagt aaattagaaa
    24901 atggctcaaa agaagtagag aagttagatt aggaggctaa ttatttatgc caatggattt
    24961 aaaaataggt gatattgttg aattaaaaaa gcaacatgca tgtgggtgta aagagtttga
    25021 aatcataaga acgggaatgg atataaagat aaaatgtact aaatgttcaa ggctaataat
    25081 gcttgataga gagaccttag aaaagagagt aaaaaagcta ataaaaaagt gataaaaatt
    25141 taacacttaa aaaatgtcaa ataccgtata ttaatatcca aaattttggt ataataagaa
    25201 ttgaagaaaa cattttcatg ggggtgcttt aattatggca gttaaatatg caaaaagaat
    25261 gcaaggatta caaggatcag aaatacgtga gcttttaaaa cttactcaac aaccacaaat
    25321 aatatctttt gctggtggaa tgccggctcc agagttattt ccagttgagg aaatgaaaaa
    25381 agtatcagtt gcagtgcttg aagaaaatgg aagatcagct atgcaatata ctacaacaga
    25441 aggatatgaa ccattaagag aaaaaatagc agctagaatg aatgataaaa acaaaactaa
    25501 tgtaaacaaa gatgatatat tagttacaag tggttctcaa caaggtctag attttgcagg
    25561 aaaagtattt attgatgagg gtgatgtaat tttatgtgaa agtccatctt acataggagc
    25621 tataaacgca tttaaatctt atcaacctaa attcatagat gttccaacag attctgatgg
    25681 aatgataatg gaagaattag aaaagatact agaaacaact gatagaataa aaatgattta
    25741 tgtaatacct gacttccaaa atccaacagg aagaacttgg ccacttgaaa gacgtaaaaa
    25801 attcatggaa atagtaaata aatttgaaat accagtaata gaagataatc catatggaga
    25861 tttaagattt gaaggagaga ctttaccatc tttaaaatca atggatacaa aaggattagt
    25921 aatattctta ggaactttct ctaaaatatt ctgcccagga tacagattag gatggacttg
    25981 tgcttctcca gaaattttat ctaagtttaa ctttgcaaaa caaggtgcag acttacaagc
    26041 atcaactata tctcaaatgg aagtaagcaa atttatggat atgtatgacc tagatgcaca
    26101 tgttgacaaa ataaaagcag tttatgttaa acgtagagat gtaatgctta agactatgga
    26161 agaagagttc ccagaaggat tagtatttac tcatccagaa ggtggattat ttacatgggt
    26221 agagcttcca agtaatttaa atgctaaaga attaatgcca aaatgtttag ataagaatgt
    26281 tgcttatgtt gctggaggag gattcttccc taatggtggt agagaaaata ctttcagact
    26341 taactattca aatatgccag aagaaaaaat aatagaaggt ataaaaaata tagcagctgt
    26401 attaaaagaa gctatgggtg tagaagctta attaaaaata ataacttata tataatacaa
    26461 gggatggttg ttgccatccc ttgtttgttt gaaaagtata taattcacct tatactaaac
    26521 tttgtccaaa atttaaattg acagaaataa gaataaatga taaaatgtgt aggtatgaga
    26581 ttttagtctc atttctctgc tctaagagtg tagagccgtt aagtccaaag ggaggtgaat
    26641 tatagtgaga aattatgaat tagtttatgt ggtaaagcca aactctgatg aagaagtaag
    26701 agaggctata ctaaacaaag ttaaggaagt tgttgcaact gacggagaaa tagttaaagt
    26761 tgatacttgg ggaactaaaa aattagctta tccaatagct aagtttacag aaggtttcta
    26821 tgtattagtt aacttcaaat cagcagtaga cgttcctaaa gagatagaca gaaacttaaa
    26881 gataaatgaa aacgtaataa gacacatgat agttgttgct taataactgg ttttattata
    26941 aaaaataggt ggtgttttta tgaatcaagt tgtattagtg ggaaggttga ctagagaccc
    27001 tgaattaaga tacatcccag gtacaggaac accagtggca tcctttacaa tagccattga
    27061 tagagactat gtaaaaaaag atggatctaa agaaactgat tttatacctg ttgaagtaat
    27121 gggtaaatct gctgaatttt gtgctaacta cataactaaa ggtagattag tagcacttca
    27181 aggatctata agagtagaca attatcagac acaatcaggt gaaaagagaa cttttacaaa
    27241 agtaagtact agatcagtac aagctttgga tagtaagaat aaatcggaaa attcatatag
    27301 agagagtgct ccagcttttg agccaagctt tgaacctcaa ggtttagacc cacaaggttt
    27361 ccaagctata gatgatgacg atataccatt ttaaaaagga gggaaaaaag tcatgatgaa
    27421 taaaaaaaga cgtaagaaaa aaagagtttg tcaattttgt gctgacaaaa acgctaaaat
    27481 agattacaaa agtactcaaa gattacaaaa gtatataact gaaagaggaa agatattacc
    27541 aagaagaata tctggaactt gtgctaagca tcaaagagaa ttaacagttg ctataaaaag
    27601 agcgagaaat atagcacttt taccatatac attagactaa aaaaatgagc cttgtgctca
    27661 ttttttatta tatgtattta tatatctaaa tgaggcgagc tataaagctc gtttttttgt
    27721 ttatatactt aaatatatta ttatttatga tataatatta tatattaagg agggttgtaa
    27781 acgtgaaaat agataaaggt tctgagataa cgagaaacat aaagataata gaatggatga
    27841 aaacagaaat attaatgagt gtaagtgatt tgtttaattt attgtttaaa ggggtaaaac
    27901 cattagatga agcaattcaa gatactttag caaatataat aatgataaca tatctcttag
    27961 caaagagatt aggaattagt tttagagatg tggattataa agtcaaggaa aaaataaaaa
    28021 ttggtataga tgaggaccat agcgttgaaa gatggtatgg agatttatct aatttaaaaa
    28081 aacatatgga taatagggag tgatattaaa ttgaataata aaattagact atctaaggca
    28141 atgtctataa tagttttagc tataataatc gctttagtaa tagcctatat tccaatgcta
    28201 ggaatattta gtatgttagc agctgttcca tatgtaatta taggtgctat tactgataga
    28261 aagtattctc ttatatctat tttggttaca ttttgtgtac tgattctatt tgcagacatt
    28321 tcttatgcat taaatatatg tataatgtat tctttaccag gtatagtaat aggaaagatg
    28381 ctaaagagaa gctttgaaca agaggacagt aataaattcg aaccgatata tggtggaacc
    28441 ataatatttg tactttcaat gcttgtatat ttttttgtat taaagacatt tttaaatgtg
    28501 aatctattag atgaagtttc taagatgata tcagaaattg taaatattca aaaaaatagt
    28561 ttttctgcaa ctgaacttaa agtgtttgat aaaatgaagc cagatgagat tgtaagttat
    28621 tttacaaata tgctaccaat gatgttattt ttacaagggt tgttatcagc gtttgtcaca
    28681 tattatttga gtgtattttt tataaaaagg attacaaaga tgaatataag atttcctaag
    28741 tttgctgatt tttatttacc gggaaatgct atactcacaa ctttcttact ttatttatta
    28801 gtcttattta ttcaaattat tggttcaaaa ttatatacag aattaataat gactaattta
    28861 cagctagttt ttaatttaat gtttgtaatt caaggtatag cagtttgcat ttatttctta
    28921 aagaaatgga taagacaggg accaaataaa attatgtttt tttcagggat aattttatgt
    28981 ttatttggat ttatgggaat atcttttgtt ggtatggtag atagtataat tgactttaga
    29041 aaggtaagaa gctataaatc cacttaggag gatagaaatg agtaataaac aaacctttaa
    29101 attaaatatg ccagaaataa atttatatat aattgttatt ggtatttcta gtataatttt
    29161 gctttattac aacttgtatg tagggtgttt atttttctgt atttttgtat atatggtttt
    29221 tcacaattgg agaactacta atattagacg tcatgaatgg acagagtata ttcaaaattt
    29281 atctttagat atagatgaaa caactaaaaa agccataata aatcttccaa taccattatg
    29341 tattttagaa tttgatggaa atatatcctg gtataatgga aagttttatg atatgattgg
    29401 tcaaaaagat ttacttgata aaaatataga ggatatagtt aaaaatctta atttaagaaa
    29461 agtattaaat gaaaataaag agatgtacac agaaataaac tataaagaaa aagaatatac
    29521 aataatttat aatgttataa aaaatgatca agaaaaaaat cctaaatatt tgatgatatt
    29581 atactggata gacaagactg aatatttaaa agtaaaacaa aattatgatg atgaaaaaaa
    29641 tgcaatgatg ctaatacaag tagatggata tgatgaagta ttaaaaagtg cagcagaaga
    29701 taagagagct ttaattaatg ttgaagtaga gaaaatactt tctgctttag aattaaattc
    29761 aaatggtgca ttgagaagaa cttcaaaaga caaattcttc ttggtaatgc ataaaaaaga
    29821 gttaaaaaaa ttggaagctg agaagttttc tattttagat acaataagac atattgatta
    29881 tggaaataat cttcctgtta ctataagtat aggaattggt atagatggag atacattaaa
    29941 tgagaaccta aaacttgcta cgggtgctct cgatttagca cttggtagag gtggtgacca
    30001 ggctgttgta aaaacaaaag ataaatttgt gttttatgga ggaaaatcta aagctgtaga
    30061 aaagaaaacc aaagtaaaat ccagattaat aggtcatgct ttaagagagg ttatacaaca
    30121 aagtgaccaa gtttatataa tgggacataa atatcctgat atggatgcta tgggagcagc
    30181 agttggtgtt tatgatatat gtaaatcctg taacaaaact gcaaacatag ttttacaatc
    30241 tgtaaatgaa tctatagaaa tatttataaa taaaataaat gaaaataatt actataaaaa
    30301 gttgtttatt ggaaaagagg aagctattga caactgcact aaaaatacat tagttgtagt
    30361 agttgatact catagaccaa attatactga atgtgaagaa ctgttaaagc tatcagagaa
    30421 ggtagtagtt atagaccatc atagaagagg tgtagagttt ataaatgatg cagtgctttt
    30481 attccatgag atatatgtat cctcaacgtg tgaaatggtt acagagttag ttcagtatat
    30541 ggatgaagat gtaacaataa ataaacttac agctgaaggg cttttagcag gtataagtct
    30601 agatactaaa aactttgcat tcaaaacagg tgttagaact tttgaagctg cttcttacct
    30661 tagaaaagtt ggagcagata ctatagaggt aaagaaattc ttcaattctg atgtaaaaga
    30721 tttcataata aaagctgaaa taattcaaag tactaaaata attaataata gaatatgttt
    30781 agcatattca agtactgaga tagatagtat aaatgttata atagctcaaa ctgctgatga
    30841 gttattaaat atcaaagaag tagaagcttc ctttgtatta ggtgaaaaag atgatactat
    30901 atttataagt gcgaggtctt taggacaaat aaatgtacat gtacttatgg aaaaattagg
    30961 aggcggggga cacatagata tagctggagc acagttaaaa aatgtttctc taaaagaagc
    31021 ttataagatg gtaaataaaa taatagagga gtatttggag gaggaagaat agatgaaagt
    31081 tatattatta aaagacgtaa aaggaactgg taaaaaggga gaaatgaaag aagtaagtga
    31141 tggatatgca agaaatttct tatttcctaa aaagatggct gtgcaagcag atagtgtagc
    31201 aataaaagaa ttaaatgaaa agaataaatc taaagaaata aaagcacaaa aagagtatga
    31261 agaagcagtt ttattaggta agcaaatgga agaaattaat atagaaatat attcaaaagc
    31321 aggagaaggt ggaagattat ttggttcaat aacttctaaa gaaattgctg agcaattaaa
    31381 gaaacaaaaa gacatagatg tagataaaag aaaaatatta ttagatgaac caataagatc
    31441 tttaggttca actttcgtag aaataaaaat acatcaaaaa gtaactacta aaataagagt
    31501 ggatgtaaaa gaaaaacagt aataactaga ggtgattaag atggaagata tgacgagaat
    31561 tcctcctcat agtgtagaat cagaacaatc aatattgggt tctatacttc tagataaaga
    31621 tgctataata acggttacag aaactataaa gcctgatgat ttctataaag aggctcacaa
    31681 aataatatat gaatgtatga taactttaag taataaaggt gagcctatag acttgataac
    31741 attaacagaa gaactaagaa aacaagggca tcttaatgat attggaggaa tcagttatat
    31801 tacaagtctt tctactatag taccgacgac ttcaaatgta aaatactatg ctgatatagt
    31861 aaaagaaaag tctgtactta gaaagcttat aaaagcttct aatgaaataa taaatctagg
    31921 atatagtgga gcaactaaga ttgaagatgt tttagaacaa gctgaaaaga gtatatttga
    31981 tatatcacag gagaaaacta gtgatgattt taaatctata aacttagttt tgatggatgc
    32041 ctatgatatg atagaaaaat tatacactaa caagagtgat gtcactggta taactactgg
    32101 ttttaaagat ttaaacaaaa aaataaatgg tcttcaaagg actgatttaa tattaattgc
    32161 agctagacca gcaatgggta aaactgcctt ttctttaaac ttggttcaaa atgcagcctt
    32221 aaaaggtgat gcctctgtag ctgtatttag tttagagatg tctaaggaac aattagtaca
    32281 acgtatgtta tcttcacaat ctagtgtaga attaaaaaaa ataaagactg gaacacttaa
    32341 tgataatgat tggcctagaa ttatagatgc tatggcagta ttatcagatg ccaaaataca
    32401 catagatgat actcctggta taaaaatatc agaactaagg tcaaagtgta gaaagttaaa
    32461 aatagaaaaa ggactagatt tagtgcttat tgattatctt caacttatgg aaggtgaagg
    32521 taataatgaa agcagacaac aagagatatc taagatatca aggtcactaa aaatattagc
    32581 aaaagaatta aactgtcctg tagtagcact atctcagtta tcacgtgcac cagaacaaag
    32641 agcagaccat agacctatgt tatctgattt aagagagtct ggagcaatag aacaggatgc
    32701 agatatagtt atgttcttat acagggacga atattatcat gctgattctg agagtaaaaa
    32761 tataggagaa gtaataatag caaaaaatag acacggtgaa actggttctg tggaacttgt
    32821 ttggcttggt gaagttcaaa gatttgggga taagttgaga gacctataat ctaacgaata
    32881 tccacagaac gtcctttctt tctacaggaa aaatgtggat acatgtcact aaatatgtcc
    32941 aaacattgaa atttagctaa aaaactatcc acactatcca cagatggcct gttgataagt
    33001 gtgaataagt agataaagca agcttttggc ttgctttatc tgtgtataaa tattaattat
    33061 taggaggaat aatatggaaa aaataagctt aaaatatata tatcccaata taattaaagt
    33121 acttgatgaa ataaacttat ttagagtaat tgacaataat ttaagagaat ctattgttgt
    33181 ttatgctaat aatgtggata atcagtatca tataaacatg actaatacaa actttggaaa
    33241 tataattaac atatgtaaat tagaaaaact tttagatgtg gataaattta tggaaaaagt
    33301 tataaaatat gaaaaagaaa taatagagaa agaagagttt tcaaaaatag aagaatatat
    33361 gttaaatatt ggagaatact aaaaaagttg aaaaaaatac gaatgtttga tagaataagt
    33421 aagtaaagtg ttcgagagta tataaaatat agaggtgaat tacatgaaaa cagttgcaat
    33481 tgttggttct caatggggag atgaaggtaa aggtaaagtt atagattatc ttgctactca
    33541 agcagatgta gtagtaagag ggcaaggtgg aaataatgca ggacatacat tagttgtaga
    33601 aggtaaaaaa tacgcattac atctaattcc atctggagtt ttaaatccaa atacagtgaa
    33661 tataatcgga aatggaatag tgtttgaccc taaaggattt ttagaagaat tggaaatgtt
    33721 taaaacggat aatataagta cagaaaatat aaaaataagt gatagagcac atgtcatatt
    33781 cccatatcat aaagaactag atgcattgtc agaagaagcc agaggcgatt taaagatagg
    33841 tacaacaaaa aaaggtattg gaccttgtta tatggataaa acagagagat ctggtattag
    33901 aatatgtgac ttaatggata aagataaatt tgctataaaa ttaaaagctc aaatagatgc
    33961 taaaaatgaa atagttaaaa atatatatgg aaaagaagaa ttatttgatt ttgaaactat
    34021 atataacgaa tatctaggat atgcagagca aataagaaaa tatgtggcag atacgtctgt
    34081 tattgtatat gatgcagtaa gagcaggtaa aaaagtattg tttgaaggag cacaagggac
    34141 tttattagat ttagatttag gaacttatcc atttgttaca tcttctcatc caacatctgg
    34201 tggatttgca attggtgcag gtatagggcc aaatatgata aaagacgttg ttggtatagt
    34261 aaaagcatat actacaagag ttggagaagg accgtttgtt acagagcaaa taaatgaaac
    34321 tggagataag ataagagaac aaggacatga atttggggta actactggta gaccaagaag
    34381 atgtgggtgg tttgatgcag ttatagttaa gtatgcagct agagttaatg gattgactag
    34441 tatttcattt atgttacttg atgtattaac tggatttgat aagataaaag tttgtacgtc
    34501 ttataaaatg ggcgataaga taataacgga tttcccagct tcattagatg atttagcaaa
    34561 atgtgaacct gtatatgaag aattagatgg atggaatgaa gatataactc aaatagataa
    34621 ctttgatgac cttccagaaa atgcaaagaa atatgtagca aaaatagaag aactagttgg
    34681 agtaagtgta gatatggttt ctgttggtcc aaatagagct caaactataa taagaagaaa
    34741 tatatttgct taatatttta tcaattaaat aatttaaaat agttgaagaa gagtatctca
    34801 agataatgtt tatagttatt agagatgctc ttttatattt gacttacatt gattttttat
    34861 taagttaaag ctacttatac atattctaac tgaaattaaa gttgaattta tgtagataca
    34921 tataggtttt ttaaaaagaa ttttgttgaa aataaatggc taaagatgat tttaattata
    34981 ttctaaaaaa agtaaaaaaa actattgact aaaaattata tagacgctat aatataactt
    35041 gtgagtgaaa gaaacgacga aaaaagtcga aaaaatgtgg tctattagct cagtcggtag
    35101 agcacctgac ttttaatcag ggtgtcccgc gttcgagtcg cggatagatc accaaaatat
    35161 ggctcgttgg tcaagaggtt aagacaccgc cctttcacgg cggtaacagg ggttcaattc
    35221 ccctacgagt caccagtatg ggactatagc tcagctggga gagcacctgc cttacaagca
    35281 gggggtcaca ggttcgagcc ctgttagtcc caccaaattt tgaattagag ataaatacta
    35341 gattatatgc tggtgtggct caacggtaga gcagctgact tgtaatcagc aggttgtagg
    35401 ttcgattcct atcaccagct ccacaaagta cataaattca aattaatata attttctatg
    35461 ttacaggata cttttaagta tcttttttta atttcatttt tattgtatta aaaatgaaat
    35521 taaacataca acaaaatttt gaattacttg caatgtgata gttttaaata taaaatttag
    35581 aatcattcta gcataatatt atgtaatata gcaaatttaa tttatagtat caataaatat
    35641 agactactaa gaatgtaaat gatttgttag attaaggtac attagatatg taaatagaaa
    35701 aataaaaagt atgttttaat ttatgtgata taagttttaa acagagaagc aagtttgctc
    35761 ttacctctct atttaaaaat aaaaaaatgt tttttaaggg ctattttatt taaatctttc
    35821 ccatctcaaa tcatctccag taaattctaa aatcctaaac ttatctaata ttctagagag
    35881 agttctttga gtgtaagtat ttcctatttg agatggtgtt aagttggttg aaataatgat
    35941 ttttttacca gcaacaagtc ttgtatttac aatattgaag atttctccac tagtaaaaga
    36001 attattaagt tcagttccaa ggtcatctat tattaataaa tcacaatcaa acaagttttt
    36061 ataattatct tcacttattt gattatttgt gtctcttcta aatttataat cttcaagaat
    36121 atctaaaatt ctaaaagaag tttgatagat tacaacattg cctttatcta acaattcctt
    36181 tgcaatacaa ttgcacatat aggtctttcc tagtccagta gaaccataaa acagtagatt
    36241 ttcactatta tccttcttaa aatcatgaac aaaattttcg cagatactta aattatttag
    36301 catattttct ctaggagata tttcaccatc agagcctttc ttaggagaaa atatattcaa
    36361 gttaaaattc gaaaaatttt cctgacttaa aatcctatct aaattagaca ttttataagc
    36421 ttcatttaca atttcttgtt tgaggcaaga acatttttct ccattaggta aaaatccttt
    36481 atctttacaa gaaatacatt gaaactttat ttccaagtaa tcaagaggta tgttactttc
    36541 agctaaaagt ctttctttct ttacttttaa agattctata ttctctttag tcttctttac
    36601 aatttcatct ttagacttag gatttaaaag tactatctta gctaaactga gacctatttt
    36661 agaaatttca tcatctatag attttatctc agggattcta ttatatacct cattttttct
    36721 atgttctaat aagagttcat tatcatctct tctcttagca tatttagcaa gtatttttct
    36781 tattttatcc tcattcattt aatcacctac ttaaactttt ctttttgact ttttttaata
    36841 atctcatcga gctcatcaga ggtatactga gtgaatgttt cgttgaaatt atgaaactta
    36901 gtttttttgt aagtattgtt gttttgagta gtattaatct gcttcttatt aatattctcc
    36961 ttaactatac gttcttcctc tttttgcttt aaatcattta aattttttat atttttttca
    37021 ttccaatttt taattatacc atttatataa gatatacttg ggtttgaaat attcttagac
    37081 ttagaacatg catttattat caaatctata tccatattaa atttatcaaa ccaggtgtcc
    37141 ataagttctt tttctgattt tgatggttgt cttgaaaatc ccaattcatt gaaaatcgtt
    37201 ttatataaaa tatatcgttc acttcttacc aaaaagcttt cttcaacatc ttttggcgta
    37261 tataaatttg aatcatacca atttctaata atgccttcaa tgtatttaac tggctttgat
    37321 gtaccagttt tatctttgac gtattcatat gcgcaaagta tcatatcggg gctcatattg
    37381 tatttattca ttatatccat aatactcatc ttttcacttg ggtcgagata tcgcccaact
    37441 attttattta tagaattaaa catttttcta atacttggat tttcagaagt agatacaact
    37501 ttatctgtat tagattttat agatgaatta ttgcttaata tattctcaat atagaaattt
    37561 tttaaatcta aaaattctat tgaataatta aaattatcat attcttcatt gtcatgtatt
    37621 tttataatct tcttttcttc ccagaatttc caagcagata acacatcaga caatggtata
    37681 tttaggtttt tggctattga attattgtcg aacttaggat tagaagtaat atcacatgct
    37741 tgtctatatc ctaaaagata aacttttacg tataacccgt ccgccattgg cataaatata
    37801 tctataaaga tattgggaat agtgatttca cctaaatcta tttcattgct ttctttaaaa
    37861 aacattttat cacctcagat taaattatat cattttttat attatatttg tctgtaaaca
    37921 aataaaaggt cacaagatta gcatgtaaat attttctaaa agtattcatt ttgaaaagga
    37981 ggagatattt tggttataaa attcaaccaa aaagtaagaa gaacattatc agtagcgatt
    38041 ctatttatgg tattaataat aggttgcttt atagaaaagg atgacagttt agaaacatac
    38101 aaagaaaaaa ctaattcaaa aataaatcag tataatatgg atgttatatt tgatgatgag
    38161 actaagaggt taatgtgcaa tcagaatgtt gactacataa ataatacaaa atcaaacata
    38221 gataaaatat atttccatat atatccgaat gctttttcca agaaagattt tgctcctttt
    38281 gaaaaaagtg aaatgacaag ggcttatcct aatggattta acgaaggata tatagatata
    38341 aagaatatat taaataataa tagtaaaatg gagtataaaa ttaaaggaga caaaaatgac
    38401 atattagaaa tagaattagg gaaagagtta aaaccaaatg aaagaatgtc attagatata
    38461 aaatataacg taaaaatacc aaattcaatt ggaagatttg gatatggaga aaatactata
    38521 aatgttacta attggtttcc tatagcttgt gtaaatgatg aaaggggatg gaatttaaag
    38581 agttatgaaa ctattggcga cccattttat agtgagacta gcgattttca tgtgaaacta
    38641 ttgattccaa ataaatataa aattgctcat acaggaaaac ttattgatga taaacatgat
    38701 aataagaaaa tgctatatga aatagaagct gaaaacgtta gagattttgc atttatacta
    38761 agtagtaaat ttgatgtaaa caaagtagat tcaaagggag tagctatcaa tacatataat
    38821 ttaaataaaa agctttctaa aaaagcaact gaagtggcaa aagattcaat aaatatattt
    38881 agtgaattat ttggaaagta tccttacaaa gaattttctg ttgtagcaag tgactttttt
    38941 atagggggga tggaataccc tatgcttgtt atgatagacc aaagtttata taatgaaaaa
    39001 aatgaatttt tactagaata tgttattgcc catgagacag cacatcaatg gtggtattct
    39061 gctgttggaa atgatgaaat aagtgaaccc tggttagatg aagcacttac tgaatattct
    39121 acaatagttt attttgaaca aaagtatggt aaagatatgg caaacaagct tataaaaact
    39181 atggagatac agacaaaaag ttatttgagt gaaaatatat ttaagccagc aaatgagtat
    39241 aaaaattcta ctgaatacag cttgaatgtt tatacaaaag gcgcaatagc atttaatgaa
    39301 ataagaaaag aagttggaga taaagtattc tttgagactt taaatgagta ctataataaa
    39361 tataagcata aaaatgctaa tggaagagct tttgtggaac tatggaatag taaaggtgta
    39421 gatataaata agattataag tgaatgtaag taaatagtga atgtaagtaa attttgtgca
    39481 gagtattaaa ttttatttaa tactctgttt ttttggtata atataattta taaggcagaa
    39541 aaagaggtga aggaatgcta aagtattgtt cagtagggag tggtagtagt ggtaattgcc
    39601 actatatagg gtataaaaat actaatatac tagtagatgc aggattaagt ggcaagagaa
    39661 taactacagg tcttaaagac attggtgtag atgcagataa attaaaagga atatttataa
    39721 cacatgagca tgttgaccat ataaaaggag caggtatatt atcaagaaag tttgatatac
    39781 cagtatttgc taatataaaa acatggtgtt ctatgaagga caaattaggt gacatcaaag
    39841 ataaaaatat gaaagtattt gaaaatgata aaacctactc attaggtgat atcatagtaa
    39901 gacctttttc aatagaacat gatgcgtctg acccagttgg atataatttt tatacagaga
    39961 gtgatgaaaa aatgtctata gctactgata taggatgtat aactcaaaat ataaaaaaac
    40021 atctatataa atctaaatta gtagtattgg aatcaaatta tgaccctaat atgcttatga
    40081 tgggttcata tacatatgct ttaaagaaaa gagtaatgtc aagtactggt catttatcca
    40141 atgaagatgc agctaatttc tgcgttgaac ttattaatga aggtacagaa tctatattat
    40201 tagcacattt aagcaaagaa aataattttc ctgagttagc gtatgagact tcaaaaggag
    40261 tattagcatc aaatgatata gtagttggac aagatgtaaa actagatgta ctatcacgaa
    40321 atgatgtttc taatgtttat gagatgaagt agaggtgaat aactttgcta aagagaaatt
    40381 ttgaaaatgt tgaggataat aaaattttat actctataaa gcagggtatg attttagcga
    40441 tacctgctat tatgactggt tcaactgctc ttgtaatttt gaatcttcca ataaaggctt
    40501 atcaagaata tttatcaggc ttgtttaatg gagaggttac aaatattctt aactttataa
    40561 atgattctac tttaggaatt atttcattaa taatattatt aacaataagc tatagttatg
    40621 gaaaaatata tgggagcaag tatacagtac ttgttcctat agttgcaatg tgctcttttt
    40681 tagtattttc acatggtaat gagcttaatt cttatataga aatatttaaa acaaagtggt
    40741 tatttacatc tataattgtt tctatgacat cttctgtatt gtttgtaaaa cttacagaat
    40801 cttttgttac aagtgtaaag tttcatacag aaggggctga tgcagacttt aatatggtag
    40861 tttcggctat agttcctttc ataatagtag tatttttgtt ttctattttt agagtggtaa
    40921 tgataagtct aattggcagt tcaaactttc aagatatatt ttataattta ttttcaaatg
    40981 tttttaataa aatgggaaca aaccttatga gtgctctgtt gtttattttt ttaatgcact
    41041 ttatgtggtt ttttgggatt catggtagta atgtattaga taccgtcgca aaaaatttat
    41101 ttgaaaatag tatggcaatt aacataaatt tagttaataa taatcaatta ccaacagaaa
    41161 tttttactaa gacctttttt gatacaatgg ttttattggg aggatgtggc tctttactat
    41221 gtttagtaat tgcaattttt cttagtgaaa aacgtataaa tgtaagaaag ttagctaaaa
    41281 ttgcttcaat accagctctt tttaatatca atgagatgct agttttcggg ataccaattg
    41341 tttttaacta tattatgttt gtaccttttg taattactcc tataatctta accgttacaa
    41401 gttatgtggc tatgtctaca ggtattgtac catgtacagt ttcagctgta gaatggacct
    41461 cacctatatt tttaagtgga tatatgtcta caggttcaat aagaggtagt ttgttgcaag
    41521 tatttaattt atgtgttggt gttatgattt atattccgtt tataaaaatg tcgcaaaata
    41581 gatatacata tatgtttaaa aataatatag aaaatttaac aaacttatta aaaaggtatg
    41641 aattaacagg tgaacagcct aatcttctaa accataatgg taaaataggt tcaatttcaa
    41701 aaatgttggc atcagacttg aagtttgcta tgaaaagagg agaaatagag cttttttatc
    41761 aaccacaagt caattataat ggaaatgttg tgggagcaga gggattactt agatggaaac
    41821 attcaatagg agggttcatt tatccaccac tagttataat tttggctaaa gaagaagggt
    41881 tcttagatga gcttggagag tatattatta ataaagcatg tattgattta aagaagagtg
    41941 aaaagctatt gagaaatcct gttaagttct ctgtaaatat atctccagac caacttgatg
    42001 accctaagtt accagagcaa ataaaaaata tcattagtag taatgatata aatcctaata
    42061 tgttaggtat agaaataaca gagcaaattg cattatcagg ttcacaaata attattgaac
    42121 gtatcaatgc gatacataac ttgggaataa agttaattat ggatgatttt gggatggggc
    42181 atagctctct tatatatctt caaaataata actttgatat agttaaattg gatggttcat
    42241 tagttaaaga gatacttaca aataaaagaa gtagtgaaat tataatgact attgtaaatc
    42301 tttcaaaaaa tttagatttt gatatagttg ttgagtatgt tgaaaatata gaccaaaggg
    42361 ataagttata tggtttagga tgtgaaatat atcaaggata ttattatagc aaagcaatac
    42421 cttttgaaga gtttgtagaa tatgcaaatc aagaaagaga gatataattt tataagagct
    42481 tgtaaattct agtaatgcat agatttgtaa taaaattaag tttaggagaa atatatgaat
    42541 ataagtatag tagtagtagg taaaattaaa gaaaaatatt taaaattagg tattgatgag
    42601 tttaagaaaa ggctttctaa gtattgtaag ttagaaatta tagagttgga tgatgaaaaa
    42661 gctccagaga atttgagtgt taaggaaatg gaaattataa aagacaaaga aggaaagaaa
    42721 attttaggga agataaagca taatagttat gttatagcct tggctattga tggtaagggt
    42781 ttatcctctg aagagcttgc taagactatg agtgatttag cagttagagg taatagtagt
    42841 ctctgtttta taataggagg ttctttaggg ctttctgatg aggttttagg aagggctgat
    42901 tataggttat ccttttctaa gatgactttt cctcatcaga tgatgagatt gatattgtta
    42961 gaacagattt atagggctta taggataaat aatggagaac cgtatcataa ataaatgata
    43021 cggtttttgt taatttgaca cttaataata ttaaaataac aaaaaagtat tgaaagtata
    43081 ttgcatttaa ggtagaatta aataaaatat tataacatta taatgtttat atggttgtta
    43141 ataaaaattt ggaggaaaat atatgataaa aataatgttg gcctgttcag caggaatgtc
    43201 tacaagttta ttagttacta aaatggaaag tgcagctaaa gaaaatggta tagagtcgca
    43261 aatttgggca ataccagaat caactataca aaatgaaata gaaaaatgtg atgtattatt
    43321 actaggacca caagttagat atgtattacc taaggctcaa gaaattgcta agccatataa
    43381 tataccagta gaagttataa atatgatgca ctatggaaca gttaatggag aagctgtttt
    43441 aaatagagcg atagaactta acaacactaa gtaatataaa ttataatgta tttttaatat
    43501 ataaattggg agaatcaaag gatggatgaa aagactatag aaatatcttt taatataata
    43561 ggttatgcag gtgaaggtaa aggattagct tttgaagcaa taaaagaggc taagaatgga
    43621 aatatagaaa aagcaaaaga attattaaaa gagtctaggg aagttataaa taaagcacat
    43681 agatatcaaa cagaattaat acaaaacgaa gctagtggaa acaggactga aataagtgtc
    43741 atacttatac atgcacagga ccacttaatg aatggcatga attttcaaca gttagctgag
    43801 gaaatcgttg atttacattt gaaattacaa gataaataaa aattaaagat aataaattaa
    43861 gagagttctt aaaagataca ttatgtgtag cacactaatt gttagattat tgattcaata
    43921 tttagtgagt aatacattag gattattttg aggactcttt ttatatttag tttaaagtta
    43981 ataaatattt attattaata atataagatt atatgttata atattataac atataattta
    44041 agaggtgaat catgttaaga aatcaaccat tatatataca agttgtagat atattaaagc
    44101 aaaaaatatt aaataacgaa tataaaattg atgaaaatat tccaacagaa agggagttag
    44161 agcaagagtt tggagtcagt aaaattacta ttagaaaagc tattgagata ttagaatcag
    44221 aaggatatgt agagaaaaaa agtggaaaag ggacaaaagt aataagtaat agccttttta
    44281 ataagctatc aaaagcagag tctttttcaa gtatactttt atctaaaggt cataagttat
    44341 ctaaagagac tatttcaatt gaaaaaataa taaatgatac aaattcacaa gtatttagta
    44401 tatttggaga aaaatgtagc aagattacta gaatgtatta tttagatagt aagccatata
    44461 tattttttac tcattatatt ccttttgatg aaaatctaac tgttgaaagt tttaataatg
    44521 aaagttcttt atatatgtac ttatatagga gaaatataaa tataactaga tttgaagatg
    44581 aattttttat agaagaatca gaactagagg tatcaaaggc tttaaatcta gctaaagaca
    44641 tattattagg aagaaaaaga aagacctatg atgagaatga taatatagtt gagctatcat
    44701 ttgcaagata taatacagaa gtacaaaact atgtaattaa atacgaaata taataagaat
    44761 cctgagttta ttgaaatgat aatatgctca ggatttttgt ttagttgtaa aaataaaaaa
    44821 tattataaca ttataatgtt tacaaccaaa cattataatg ttataatatt tttaataaac
    44881 aagtgatatt atcaattaca taaaaattag tttatgttat gaaatcgtta ttgcattata
    44941 aataaaggat aatatcaata atacaggggg ataactatgg aaaaattcgc aagtttttta
    45001 gagcgcaaga ttatgcctgt agcagcgaag ataagtaatc agagacatat gagagctgta
    45061 agagcaggta taatagcaac attaccacta acaatagtgg ggtcattttt cacaatatta
    45121 ttaaacatac ctattgatgc ttatatggat tttatagctc catttaaagc aatattagat
    45181 ataccattta gatatactgt aggattttta tctctatatg ctacttttgg gatagcatca
    45241 tcacttgcta aaagctacga tttagataca acaggtgttg gtatgcttgc agttatggca
    45301 tttttagttt ctacaatagt tccaactcaa gtagttgaac cagtaggtgg agttatagaa
    45361 gctggtagat ggatggatat ggcaaaactt aattcgccat cattatttgg agctatcgta
    45421 acatcattaa ttacagttga aatatatcgt tttatgaagg aaaagaatat tacaattaag
    45481 atgccagcag gagttccagt agaggtttca aattcattta ctgcattatt accgacagca
    45541 gtaattttaa tgttattctg ggtaataaga catattctag gatttgatat aagtgttgca
    45601 ctaagcgcta tgttatcacc tttaaaagga ttcttatcag gaaatagttt aggtggtggg
    45661 ttactgacag tattcctaat aactttattc tgggtacttg gaatacatgg ttcagctata
    45721 atgggaccag taataagacc tatatgggac atgtctatag cagaaaatat aactgcattc
    45781 acagaaggag tatcagctca tgcaatgcca aacatattta cagaacaatt tttacagtgg
    45841 tatgtatgga tagggggagc aggggctact cttgcattag ttgtaatgtt tatgttttct
    45901 aagtcacaat atctaaaaag tttaggtaga ttaggatttt taccagggtt attcaacata
    45961 aatgaaccta taatatttgg agcaccaata gttatgaatc caatactagg gataccattt
    46021 ataattggac ctataataat gacaattcta tcatatgttc ttactattac aggggttata
    46081 cctatgatgg tagcaagatt accatttgca atgccagcac caatagcagc agtaatgagt
    46141 actaactgga gtatactagc aggtatctta gtattaatca actttgtgat atcatttgct
    46201 gtttattatc cattcttcaa agtttttgaa aaacaacaat tacaaagaga acaagaagaa
    46261 atgagtgaaa atttataaaa atacatagat atagaggttt tgtactatat aaaatataga
    46321 aaaagcttat aagttattaa gggtgaaggt aggaggaatg acctttgcct ttaattttat
    46381 atattttaaa taatgtaatt tgaggaggag aaagatggaa agaagattag gaatatcagt
    46441 atatccagag catagtaaca tggaagatga taaggcatat ataaaaatgg cttcaaaata
    46501 cggatttaaa agaatattta tgtgcatgct ttcaattaca agacctaagg aagaagttaa
    46561 aaaacatttt aaagagatta taacctatgc aagagaatta ggatttgaag taatccttga
    46621 tatagctcca aacatatttg atatattaca aatatcttat gatgatttaa cattttttag
    46681 tgagttaaat gctagtggaa taaggcttga ctatggatat gatggaagta aagaagctat
    46741 gatttctttc aatccatatg atttaaaagt ggaactaaat atgagtaatg atgtagcata
    46801 cttggataat ataattactt acaagccaaa tgtaaataat atatatggtt gtcacaattt
    46861 ttatcctcaa aggggtacag gcttgccata tgattttttt gtaagatgtt caaatagatt
    46921 taaaaagtat ggaataagaa ctgctgcttt tgttaattca aaatacgcaa caatagggcc
    46981 ttggaatata aatgatgggc tgtgtacgct tgaaatgcat agaaacttag atattgctac
    47041 agcaaccaag cacttattcg caacaggatt gattgatgat gtaattgtag ggaatgcata
    47101 tgcaagtgaa gatgaattaa aaagaatgtc tgaagtaaat aaatatcaag ttgtatttaa
    47161 tgtagtaact gaagaaaaaa cttctgaagt agaaaagaca ataatggcaa atgagcaaca
    47221 ctttagaaga ggggatataa ccaaccaagt agcaaggtct acagaagtaa gaaaaaaata
    47281 tgctaaagaa aatttcccta agcatgataa tacgaccatc tttaaaagag gagatgtagt
    47341 gattggtaat gatgactttg gaaagtataa aggggaatta cagttgatac ttcaagattg
    47401 tgcagatgaa agaaaaaata aggtgggtag aataaaggaa gacgaaataa tacttttaga
    47461 ctttattgaa ccttggagta aatttaaact tattttagag ggagagtaaa gtgaagatag
    47521 acatattaat aaaatgtgga aaaacagtag ataaaagtat aattgatata gctatattag
    47581 acgataaaat aatagaagta aaaaatcaga tagatgattc taagtatcag gcaaatcaaa
    47641 ttataaacct agatggagaa aaatttatta gtgctggttg gatagatata catacgcact
    47701 gctacgaaag actagacctt tataaggatt atccagatga ggttggaata aaaagcggtg
    47761 ttactactgt agtagatgca ggaacaacag gagctttaga tataggtaaa ttttatgaag
    47821 atactaaaaa atataaaaca aatgtatatg ctttaataaa tattgctaag cagggaataa
    47881 catctcaaga tgaactttca agtatgatga atattgatga atatgaactt agaagagcag
    47941 ttaaaaaata caaagatttt gttgttggaa tcaaagctag aatgagtaaa agtgttgtaa
    48001 tttcaaatga tgttgaacct ttaaaagtag ctaaaagaat aaaaaatgaa cttaatctac
    48061 ctatgatggt tcactttggg agttctcctc caacgataga ggatatattt gactatatgg
    48121 aaaaaggaga tatattaacc catatatata atggaaagcc aaatggaata cttagaggca
    48181 atgaggttaa aaaagaaatc atagaagcta gagaaagagg tataatcctt gatgtaggtc
    48241 atggaaccga aagttttagt atggatattg caatgaaatc taaggatgca ggaatttttc
    48301 cagacactat aagtacagat atatatatta aaaatagaat aaatggtcca gtgtataatc
    48361 tatctacaac aatggaaaag tttatatata tgggctattc acttgaagat ataatagata
    48421 aagttactaa aaatgcagca gatgcaataa gtttaaaaaa taaaggttta ataaaagaag
    48481 gatatgatgc tgatttaact atatttgatg tggttaatga agaaaaagaa cttcaagatt
    48541 ctttgaataa gtcagtaatc acaagtacgt ctataaagcc aagagcagta gtagtcaatg
    48601 gagaatattt aaatataggt aagtctatag gagagaatga gtagaaggga gaaactattg
    48661 tatgagtagt atatttgaaa aatactctgt aagacgtgtt ataaatgctt caggcaaaat
    48721 gactatacta ggagtatcaa cagtagataa ggaagttatt gaagcaatag gtgaagggtg
    48781 taaaaatttc tttataatag aagaacttat aaataaaaca ggtgaatata tagcaaattt
    48841 attagggtgt gaaagtgcac taatagtttc tagtgcatct gctgggattg cacagagtgt
    48901 gggagcaatg atagcaaaag atgatatgag tctagtttac aatgtaaata atcctaagaa
    48961 acaagttaaa agggaaataa taattccaaa gggacataat gttgattatg gagttccagt
    49021 agaagtaatg ataagacttg gaggaggaga agttgttgag gccggttatg ctaatatgtg
    49081 taacaaggaa catataataa gagagataaa tgaaaataca gcagcaatta tgtatattaa
    49141 atctcatcat tgtgttcaaa agagtatgct aagtgtatta gatgcagtac aagttgctaa
    49201 agaatataat ttacctttga tagtagatgc agcagcagaa gaagatttaa atgtatatta
    49261 tgaattggga gcagacctag tgatatacag tggtgctaag gctatagagg gtcctagctc
    49321 agggttagta ataggtaaga aaaaatacat agataatgta aagcttcaat caaagggtat
    49381 aggaagagct atgaaaatag gaaaagaaaa tattgtaggt ctaacatgtg caattgagag
    49441 atatataagt aatgataaag taactttaaa agaaatggag gataagctaa atccatttat
    49501 agacaagata aatactataa agggagtgag tgctagtata actagggatt cagcaggtag
    49561 agaaatactt cgtggagaaa ttgactttaa tgaagatata ataaataagt caacacaaca
    49621 aattataaat gaacttagaa gtggagagat agctatatac actagagact ataaggctaa
    49681 tgaaggcaag attgagattg atataagaag tgtaactgga agtgatttag atactattta
    49741 taataggata aaaaatatag tagaaagtta gagtgtagag taagtgacat tataatgatt
    49801 taataaatta aattttacca atggagggga taaatatgaa ttgtatacca caatattata
    49861 aaaatagagt ttgtttaaat gttcttgcag gaagcataaa aaatgctaaa gaagtatatg
    49921 atgcttgtga cgggcatgta ttgataggaa tactatcaaa gaattatagc actgtagaag
    49981 cagctattga agatatgaaa aaatactcaa aggaaataga taatgcaata tctgtggggc
    50041 ttggagctgg agacccaaat cagtcaaata tggtatctca gatttctaaa gaacttcagc
    50101 cacaacatat aaatcaagtg tttacaggtg tggctacgtc tagagcattg cttggtcaaa
    50161 atgatagtat aataaatgga ttagtttctc caactggtaa accaggattt gtaaaaatat
    50221 ccacaggtcc attaagttca aaagaaaaag atggtatagt tcctatagaa acagcaatag
    50281 ctatgatgaa agatatggga ggaagctcaa ttaagttttt tccaatggga gggcttaaaa
    50341 caaaggatga atatatagaa gttgcaaaag cttgtgcaaa gcataatttt tatttagaac
    50401 caacaggggg tatagactta gataacttca aagagatagt tcaaattgca cttgatgctg
    50461 gtgtagaaaa agttatacct catgtttata catcaataat tgataaagaa acaggtgaaa
    50521 cgaaagtgga agatatagga gtactttata aaataatgaa agaattattg ggatagattt
    50581 atgaatatat tagcttttgg tgaaataatg atgagacttt cagtacctga ctacaagttc
    50641 ttaacacaaa ccaatgaact aaattatata actacaggca cgggattaaa tatattatct
    50701 ggacttaaaa actttggata caatacctat atgttgacaa agttgcctaa taataatgta
    50761 ggtaaagcat caagtgcaaa tataagaaag cttggagtaa aagatgactt tatcacgtat
    50821 ggagggaatc atataggagt gtattttctt gaaaatgggt atggagaaag accttctgaa
    50881 gtgacttatt tgaataggtt aaatagttca ttttgtgaga gtaaattaca agattatgat
    50941 attgataaat gtttagaaga aatggatata gtccatattt gtggtattgc tatgcaactt
    51001 acagattcag ttaaagagat tgctttagaa ctagctagaa aatcttatga aaaggggata
    51061 aaagtattct ttgattttaa ttttagacaa tcattaaatc cagaaaggac ttatgatgat
    51121 ttagtaaatg attataagaa aatactacca tattgtcatg ggctgtttgc tagttataga
    51181 gatataaata agatacttaa tatagatagt ctaaatataa aatctagttc agaattgatt
    51241 aaaaaagagt atgatataga tattgtagct ggaactatga gaagatttga tgatatgaat
    51301 aatagattta taaagggata tatatcaaat gatgatggtt attttgaaag tagggagtat
    51361 aagctagaaa tatatgacag ggttggtgcc ggagatggat atgtagcagg aattatatat
    51421 tcatatttgg aaaatatgaa gagtgaagat atagttgagt ttggggttgc tagttcggtg
    51481 ttagctcata caacttatgg ggataattct ttagtaagca caggtcatgt acttagactg
    51541 atgaataatg aaaaaataga tatattgaga taatacattt gtatataata aaatttttat
    51601 cttttttaga cacatttttg acttgcttat atttgttaaa cagagttatt tttaatgatt
    51661 ataaatatta aattaaaagt taaataataa aatagtagaa aatagcatta actatagaaa
    51721 aaatggttaa tgctattttt atcgttaata ttgattaatt ttatattata gaaatgaact
    51781 tttctaatgc tggatttgta ttttttttat tccatacaac tacaacttca attctatttt
    51841 ctacttcttt tataggtact attgcaatat cacgttctat cattgtatct gtaacacaaa
    51901 atgaaggtaa aatggatatt cccatgttag aagataccaa tgattcaata gtattaagat
    51961 tatttgaacg ttgtaccaca ttaggtacaa accctgcttc acgatatcta ttctgtatca
    52021 ttgacttttg tccatactca tcccctgttt cctttacaaa aataaattta tcatgttgta
    52081 attcaattaa atcgatagaa tcctcattgt aaagtggatg accaggaggt aaaagagcta
    52141 ccaaagaata tctttctaca gttttatatt caaaatcttt taatggttga ttttctggat
    52201 taataacaaa agcgaggtcc atagaattat tttttactaa gtttaaaagt tcggttattt
    52261 cattagtaca aatatccata gatatattag ggtatgtatt agaaaattct tttaaatatt
    52321 gttgaagttt gtttttttca tatcctatta aggttccaat gctcaaagtt ccaaaaaaac
    52381 catgtgcaaa ttgttgtgtt tttgcaatag catcattgat attttttatt atagtacgtg
    52441 cctcagataa aaaaactttt ccagctggtg ttagttcaac atgacgttta cttcttgtaa
    52501 atagagttac atttatttgt tcttctaaag cttttatttg ctgtgtaaca gcagtttgag
    52561 aaatataaaa tttttctgct gttttggtaa aacttagatt ttctgctaca gatataaaat
    52621 attctaattg tttagtattc aaataatcac ccctatttaa acttgattgt ttgtaaacct
    52681 tatttatcta taattaaagc ttattatagt ataagaaaat ttacttttaa agaatattag
    52741 aaaatatttg tat
//
LOCUS       DAYAPA010000010        52372 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.010, whole genome shotgun sequence.
ACCESSION   DAYAPA010000010 DAYAPA010000000
VERSION     DAYAPA010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 52372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52372
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.010"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(<1..2044)
                     /locus_tag="ACGI59_000533"
     CDS             complement(<1..2044)
                     /locus_tag="ACGI59_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HFG9738342.1"
                     /translation="MKAGEIEFLSYLEGSNKSFVVPVYQRNYNWKKEQCKRLFDDLED
                     IIDSGFRTHFLGSVVSIYSMGKEYLIIDGQQRVTTVSILLIAMCQIIKEFGESKGIIT
                     TEEQIKECYLINKHSRDKDGKIKLKPIAEDRKAYYELFEEEMNTDRNSNIVSNYLYFY
                     NRIKEDNISIDDLFDAIQKLTIVEIELKNGEDDPQLIFESLNSTGLDLSEADRVRNFV
                     LMGRSSKLQEEFYNNYWYKIEKNTSSNVSSFIRDYLTIKERSIPNKNKVYFSFKRYVS
                     DNGIDIEILLKDLLKFSNYYREISYSCTDDNDVNKVLKRINKLEIFVSYPFILELFDD
                     YNEGIINKKVLVESLNLVECFILRRIVCDVPPNALNKVFMNLGREIKKFSDYKENYSN
                     ILRYIFINKKSSQRFPTDSEFSNAFETKDIYNTKSKNKLYILERLENYSNKEIVDLEN
                     LINNNMLNIEHIMPQTLTNKWKESLGDNYKEIHDKYLHTIGNLTLTGYNSKLSNKTFE
                     EKKEMEDGFKDSRLYLNKYISSIDKWNEEEIKNRTKILLDRAVEIWKYPSVTYEPPSN
                     SENIFTLEDDDVNFTNESIVSFSILGESYKAKNWTEFYEQVALTLYDLDSVRFNSIVD
                     KTYKRDFIDNIKSRDAYKLRKGIKISENIYLETNLNTEAKLNIIRFLLNEFDIELNE"
     gene            complement(2425..5760)
                     /locus_tag="ACGI59_000534"
     CDS             complement(2425..5760)
                     /locus_tag="ACGI59_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFG9738343.1"
                     /translation="MGNFDFLQKKKNFNSFNTACLEAEKSILVSPSTCATITRRALEL
                     AVKWLYANDSDLVLPYQDNLSTLIHNNSFIELIDYDMLPLLKYIVKLGNLSVHTSANI
                     ERGEAILSLNNLHQFVSWIDYCYSDEYTAEDFNEDLLLHGEEKRTRPDELKNLYERLS
                     SKDKKLEEVIKENEELRKSLTEKRKVNTVNYDFKIDEVSEFETRKRYINIELKLAGWE
                     FGKDIGEEIEVQGMPNESGVGYVDYVLYGENGKPLAVVEAKRTSKDPKIGQQQAKLYA
                     DCLEKRYSQRPVIFLTNGFDMYIWDDYSDRKVYGFYKKSELQLMMDRRKSKKSLSSVT
                     INDEISNRYYQKEAIRAVCEALENKQRKTLLVCATGTGKTRIAISIVDVLSRHNWIKN
                     ILFLADRKALVKQAKKSFTKLLPNLALCNLLDSKDSPEDARMIFSTYPTMMNAIDDTK
                     SKDGKRLFTPGHFDLIIIDESHRSIYKKYKSIFDYFDSYLMGLTATPKDEIDKNTYSV
                     FDMENGVPTYAYEYNKAVEDGYLVDYTSIEFKTKIMEDGIKYDELSDEEKEEYENTFN
                     DDESIGDEIGNNAVNEWLFNSDTIDLVLNKLMTEGLKIEGEEKIGKTIIFAKNTKHAR
                     AIVERFNKLYPKYGGNFVKAVDYSINYVDSIIDDFSDKNKLPQIAVSVDMLDTGIDIP
                     EILNLVFFKKVRSKTKFWQMIGRGTRLCPDLLGVDMDKERFLIFDFCNNFEFFKFNPK
                     GFEGNKAETLTEKLFNIKVSMVKELQDIKYIEGEYAELRKELLEELITSVKALNEDSY
                     IVRMNLSYVHKYKNENVWSNIGAVAQNEIREYISPLITSYSDDELAKRFDVVMYNIQL
                     AYLQNNNASKGIRHVMATAEKLSKLGTIPQIQQQKYTIEKAMTENFWEDSDIFEVEEV
                     RISLRELIKYLEKSSQKIYYTSFEDMIVAEDRNDSVYNANNLKNYKKKVEYYLNSHKD
                     ELAIFKLRNNKKITKQDVETLEEILLKQLGNCDDYKKEFGDTPVSQLVRRLVGLDREA
                     ANEAFSEFLNNKSFNTKQIHFVKLIVDYVVKNGFIEDNKVLMEDPFRTVGSIIDLFEN
                     HIEERNKLIKTINKIKENASEIG"
     gene            complement(5810..6238)
                     /locus_tag="ACGI59_000535"
     CDS             complement(5810..6238)
                     /locus_tag="ACGI59_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9738344.1"
                     /translation="MVIRNKLSWNSIEEVNRDFGSCLYDLQNFKILYDKEEMPELWAR
                     YIKEGFKSYMVSFLELTKAMLYYKSIDLNIKSKNFYDYLLACEYHNLLPKNSSIVIET
                     LRKLRNDDSHGYDIPQFEDMYELFTENEEVFVSIRNSCKK"
     gene            complement(6228..6608)
                     /locus_tag="ACGI59_000536"
     CDS             complement(6228..6608)
                     /locus_tag="ACGI59_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFG9738345.1"
                     /translation="MRNIPNEIKNSIINVFPKENTLAIIIFGSYGTDKHTSESDIDIA
                     WIPIKKVPITELATKTEALRNILDIDVDLKIVTDNYTIALKKAILEGEVIYESKDFKD
                     YINKFYFENSDVIDILDWRDMYGN"
     gene            complement(6641..7813)
                     /locus_tag="ACGI59_000537"
     CDS             complement(6641..7813)
                     /locus_tag="ACGI59_000537"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFG9738346.1"
                     /translation="MEYIKLNELCYINIGKTPSRNTSDYWGSGNRWLSISDLKEKYIS
                     KSKEEITDLAVEKANMKLVPKNTVVMSFKLSIGRVAILKEDMFTNEAIANFQIKNNEL
                     ITSEYLYYALRTLNFNNTDRAVMGATLNKSKLNDIKIPYFTICIQNKMVEVLNKAQEL
                     INKRKEQIEALDELVKSRFIEMFGEPINNTKKFDMKKIKEISIYLKRGVSPKYVEQSN
                     IKVINQKCIYWRNLKVENCKYYDENLKEKIEDIFLKQNDILINSTGTGTLGRCVKFNK
                     IDNSEYIADSHVTVLRSISNNLNSDYFAALFEFTNIQDTIYRKCVNGSTNQIELSVSK
                     LGEYLIMVPPIEFQNQFSEFVKQVDKLKLKIETNLKELEDNFNSLMQKAFKGELFN"
     gene            complement(7817..9280)
                     /locus_tag="ACGI59_000538"
     CDS             complement(7817..9280)
                     /locus_tag="ACGI59_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFG9738347.1"
                     /translation="MITGELRSKVDKIWETFWTGGLTNPLEVIEQFTYLLFIKGLDEV
                     ETKNEAEATLLGVSFERIFDDEHQHLRWSKFSNEGNSEKMYEIVQNEVFPFIKKLHGN
                     KDSAYAKYMGDAIFKIPTPLMLSKIVDGISNLELSKDRDTKGDLYEYLLSKVATAGTN
                     GQFRTPRHIIDMIVRLIKPTPEDIIVDPAAGSAGFLVSSQQYLRDNHSSLFLVQGLKE
                     HFNNGMFYGFDMDRTMLRIGAMNMMLHGVDNPNIEYKDSLSEVNTDKDKFTLVLANPP
                     FKGSLDYEAVSADLLKVTKTKKTELLFLALFLRVLKIGGRCACIVPDGVLFGSTGGHK
                     SIRKEIVDNHKLEAIISMPSGVFKPYAGVSTAIMIFTKTGTGGTDKVWFYDMKADGYS
                     LDDKRNEIKDSDIEDIIKRFDNLDGEVDRKRTEQSFFVDVEEIRENGYDLSINKYKEV
                     VYEEVVYDAPSVIIGRVRELEKEIAVGLEELERMLEV"
     gene            complement(9790..10659)
                     /gene="gatY"
                     /locus_tag="ACGI59_000539"
     CDS             complement(9790..10659)
                     /gene="gatY"
                     /locus_tag="ACGI59_000539"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HFG9738348.1"
                     /translation="MALVTTKQILLDAQEGHYAVGAFNVENMEMVMAVIEAAEELKSP
                     VILQTTPSTIKYAGLDYYLANVKVASEKASVPVAMHLDHGSSFGLAMQALRVGYTSIM
                     IDGSHESYEDNISISKSVVDACMPSDIPVEAELGKVGGKEDDLDGGDGGAYTDPLEAK
                     EFVERTGVSSLAVAIGTAHGLYKGEPKLDLDRLSEIRDVVSVPLVLHGGSGIPDEVIK
                     ESIKRGICKVNYATELRIAYSNGVKDVLNSDPEVIDPKKYGKKGLESVKDFVKSRMEV
                     CGCVGKAIEIVKC"
     gene            complement(10679..12595)
                     /locus_tag="ACGI59_000540"
     CDS             complement(10679..12595)
                     /locus_tag="ACGI59_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFG9738349.1"
                     /translation="MGKKMILGVTGCPTGIAHTFMAEEALKNSARELGCDIKVETNGA
                     IGVENKLTAKDIEMADAIIVACDKNVDMDRFNGKPVIEVPVKEGIHKANELIQRCIDG
                     KVAIRKGNLSSSKIAEEGNLSFGQKLYKDLMNGVSHMLPLVVAGGVLTAISFLWGIYS
                     FDPNSEQYNQIAATLKSVGGYSMNLMVPVLAAFIAQSISGRAGMLAGLVGGMISFDTG
                     SGFLGGIISGFLAGYLVKLFVHLLRKLPRQLEGLKSIFIIPIVSVGLVGVAMLLLGGP
                     CSALNNAMMNFLSGLQNSSPIILGIVIGCMSAFDMGGPVNKAAYVTGTMLLGQGNYLF
                     MAGVSAACITPPLVIAIASTLFKNRFTEEDRAAGLINYILGSTHITEGAIPFAAKNPL
                     KVLPVLMIASSISSILTYIMKIEVPAPHGGFLVLGLVNKPLLWVGCIFVGSLVGAVLY
                     MMVTPKVVNEKNTIENTNVTKDEHKNESAESENVSNNISLYSEETVILDVKGKNKTDV
                     IDEMIGILDKSSVLLDKKKFKEEILKREEISSTGFGMGIAIPHAKTDAVKVPRVAVGV
                     SKEGFDFESEDGSLAHIIFMIAATDNGDNLHLKTLSQLSSKLMNEEFLNELLNSKTSR
                     EIVAKLNNEEIKSV"
     gene            complement(12598..13518)
                     /locus_tag="ACGI59_000541"
     CDS             complement(12598..13518)
                     /locus_tag="ACGI59_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HFG9738350.1"
                     /translation="MIYTLTTNPAIDMNISTNGIKRKLVNRTSNAVYSPNGKGLNVTF
                     VLGHYGIESKILGFFGGFSGKYIVEESEKRGFDVLPTWVEDTTRINIFLNDGSDEFKF
                     VNSGSYVNEKQKLDMLDKIQSLADISYLSISGSLPPGIDDNYYEDIFKICKNKSIKTI
                     LDISSPKLKELLQYNPYLIKPNDEEIKDIFGIIVRDEEDIKDVLKLLHLKGAQNILLT
                     LGEKGSYFYNGKSIYYASAQPVTVVSSACAGDSALAAFLSIWLENPEHIEEALKRSAA
                     TGASVAESNGIGNLENVEDYIKNIRVRKVV"
     gene            complement(13620..14372)
                     /locus_tag="ACGI59_000542"
     CDS             complement(13620..14372)
                     /locus_tag="ACGI59_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFG9738351.1"
                     /translation="MIIELNKSNSENLTKTELEIIKFINENESILPELSIVEIAHETY
                     SSPATVSRAIRKCGLNGFNELRYRLTVKDENDDIQNMGDIINKSFIEAQRVIEQISLT
                     TLINIIKTINKSSKIYILARGLTEYVAKEFSLKLQLLDFNVVFINDPNIMKIKSKSIK
                     KDELIIIFSLNGNTIELVESAQNANLVGAKVITCCCSESAKLIKYSDLYIVGYKHEHI
                     SISEYEVSSRLPLYIISRIIIDYMVRYNNSEN"
     gene            complement(14764..16209)
                     /locus_tag="ACGI59_000543"
     CDS             complement(14764..16209)
                     /locus_tag="ACGI59_000543"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFG9738352.1"
                     /translation="MIKLGKFPKGFLWGGAVAANQLEGAWNEGGKGWCLADVHMYDKD
                     RDISKPYESDMTIERIKFAMKDNNGYYPKRSGIDFYHTYKEDLKLLKELGLKCFRFSI
                     NWARIFPNGDDLEPNEEGLKFYDNLINEVIDNGMEPIVTLLHYETPLNLVLKYKGWSN
                     RKVVDFFVRYAELILNRYKNKVKYWIVINQINLIHYESFNSVAFCVDQVDNVEETRYQ
                     AIHHQFIATALTKKLGKEINSDMMIGMMMADCTSYPEDCDPENVVFALKRNRLQYFFT
                     DVSFRGEYPVYIKRFFKENNINIVMEEGDDKILKENTMDFLALSYYYSTTVSASKNTM
                     NPTDNIKNPYLKANPWGWAIDPKGLYNTLSQYYDRYQKPIMIAENGFGMHDKFEDGKV
                     HDSYRIDYLGKHIEQIGEAIADGTEILAYCMWSPIDIVSCSSQEMEKRYGFIYVDIDN
                     NGNGSKERYKKDSFYWYQNVIRTNGEELREK"
     gene            complement(16231..16584)
                     /locus_tag="ACGI59_000544"
     CDS             complement(16231..16584)
                     /locus_tag="ACGI59_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFG9738353.1"
                     /translation="MTIEEHIIDEELVEVAMQIILRAGEARTEIKHALNDLEKFDYRN
                     ADLKLAKAKGFMTEAHRAQTKIIQGEAGGEKRAHSLLFAHAQDTLMTIFSELNITMSL
                     VGIVKSIEYKVNNSK"
     gene            complement(16613..17845)
                     /locus_tag="ACGI59_000545"
     CDS             complement(16613..17845)
                     /locus_tag="ACGI59_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFG9738354.1"
                     /translation="MNKFIDFLSEKFTPVVNNMTKNIWVQSVQSTIMKVLPMVFVGSL
                     VTIVSVLKNYISFLPDLSPINQYTFGLLGLFIAFLLPMETMKNKKFESMSVVAGLAGA
                     GLMLMMIRPEITNEGAIFNFNRFGGEGMLVSLVAGLFSGLIMSLFGSFSFFGEDSALP
                     DFVSKWFDNMLPITVIMFVGWGLIYGLNFDMFEIIVRVFKPVSDIAQTLPGFVLLNFL
                     VVFIYSLGISGWVLSPITYSIQLGAIAANAAAVASGGVATNIYVYEVIQTGWITLGGL
                     GATLPLVIMMCLSRVGRIKAIGRACIVPSIMNINEPVIFGAPVAWNPFLMLPMWIGTV
                     VISITTYLSMSMGFVTIPSKVFNLWYVPFGISTFMVNQDVRGLIVLAINIVLLFMIWY
                     PFFKAYEKEEIKLESQEV"
     gene            complement(17868..18185)
                     /locus_tag="ACGI59_000546"
     CDS             complement(17868..18185)
                     /locus_tag="ACGI59_000546"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFG9738355.1"
                     /translation="MKNIRVLLACGSGASTGFMATSMRKSAKKKGIEADIKARSESEI
                     DKYLSEIDCLLLGPHLDYMKEEIQQKANEYNVPVACISKTAYGRLDGAKALEEALELI
                     SNK"
     gene            18427..20340
                     /locus_tag="ACGI59_000547"
     CDS             18427..20340
                     /locus_tag="ACGI59_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFG9738356.1"
                     /translation="MKTSKKELFLNFIKTQHDWIDSSTLANYLNVSTRSIRKYVNEIN
                     SNGAFILSSKKGYKVNLNTNCQTKIDSSENISPDNRLNLILKELIVNSNGINIFDLSE
                     ELFVSPATIEGDIVKANKFIGSYNLKIKQSKFLLKLVGNESAKRKLMSSIIFKETGSD
                     FLSLFDVQKIYQEYNLTKLKENIIYILKKYNLFINEYAINNILLHLMITIDRIKKNNY
                     IDSVEVVNYIDNNVDINIAKDISNFLESEYNITLTSAELYYLVFQLTNKTTVLNYNQM
                     DTKSLSNYIDEHFVKLTKKIIKNVYDLYLIDLSDEEFVVKFTLHVKNLISRAKNNQVL
                     RNQIPQKLKDSYPLIYDISVYICNQIQTLENVDIDEDEISYISLHVGSFFDRQKLLED
                     KVLCALITPNYYDLQFKIVRDLEKRFNESIEIIQIFSDTHNLDFDNKVDMVITTLPIN
                     DRCPIPFVYVNPYLNRKDYDNIQSKFIQIKDRKNILTVQNHLEMYFSESLFMKNIYLD
                     SAKDYIRFMGNILYENKYVKPNYIDDVLIREKMSSTAFNNNVAIPHSMKMDALKTGVC
                     LIVNDKPVKWGEEKVQIIAMIPINEKEKEKFNYIFESFIEILSEWNNVKELTKADNYS
                     SFMNRIAYLIQNI"
     gene            complement(20463..21617)
                     /locus_tag="ACGI59_000548"
     CDS             complement(20463..21617)
                     /locus_tag="ACGI59_000548"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFG9738357.1"
                     /translation="MKILISIDSLKGSLSSIEAGNAIKKGILKVKEDAQVKILPLADG
                     GEGTVDALVQGMNGKKETIEVTGPIAKKVDATYGLLKNTSTAIIEIAQASGLTLVPTE
                     LRNPLYTTTYGVGEIIKEAINKGYRNFIVGIGGSATNDAGIGMLQALGFEFYDKNNKL
                     VGLGGKVLNEIRHIKIENRLKELDECKFKIACDVNNPLFGKNGAAYIYGSQKGATSEI
                     IEKLDNGLRNFSKVVKNYLSKDVANVEGAGAAGGLGFAFLAFLDSKLESGIKIILEEI
                     KLEEELKDADFVITGEGRLDNQTAMGKAPIGVAKLAKKYGVKVIGLAGATTEDAVKCN
                     EEGIDAYFSIVNRAMTIEEAMDKATASENMTATTTQIFNLITSIQQSKNM"
     gene            complement(21896..24511)
                     /gene="mngB"
                     /locus_tag="ACGI59_000549"
     CDS             complement(21896..24511)
                     /gene="mngB"
                     /locus_tag="ACGI59_000549"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HFG9738358.1"
                     /translation="MKKNVHIVPHMHWDREWYFSTEESRILLVNNMEEIMDMLENNHN
                     YPYYVMDGQTAILEDYLAVKPECKERIKKLVQEGRLIIGPWYTQTDEMVVGGESILRN
                     LLYGIKDCDEFGEYMKIGYLPDSFGQSAQMPQILNGFDIKYSMFWRGCSERKGTNKTE
                     FNWKSDDGSSVLVQILPLGYAIGKYLPMNEDELRTRMDKYLPVLDKGATTDHIILPNG
                     HDQMPIQKNIFEVIYKLKECYPDRKFFLSRYENIFKELEKNEDIDTIKGEFLDGKYMR
                     VHRSIFSTRMDIKSANARIESKITNILEPLASIAYSLGFEYHHGLIELIWKEIMKNHA
                     HDSIGCCCSDKVHQEIMNRFFLAEEKVDQLITFYKRRIVDAMSCEMALDKLIAFNLMP
                     YDRNEIVSAQVITKMKAFEIFDKDNNKLDFEVVHKEVIDAGLIDRQIVHYGNYDSFMV
                     YTINFKDKIPAMGYKAYMIKEVEHMLEKAYEVCDMVDNDFYTIQVNENGTLKIFDKKL
                     NKTFENVLLMENGGDEGDEYDFSPLPNEKLIFNDNIVANSTIKKNRFNNEIKINYRLK
                     VPKDIQARKNNRADSFIDFDIVINVPNDKAIIDVVFNINNQARDCRVRTYIPTNIASK
                     FSVSDNQFGHIKRSVYDDAMEVWQQEDWSERPDSIYPMLTFVGLSDKEHGVAVLTNST
                     REFEIVGEKFDTIAITLFRSVGFLGKEEMARRPGRPSGIKLPTPDSQMIGDIKIDLAI
                     VTHEKSTLEANVANMAKEYLTKIQTYNKMPYNAMKLNQSEIEVDYSYSLLKETNKDLV
                     LSVVKKAEKEEALVVRFYNSKEETKSANIVFNKNIKTAKWINLNEKELESISIDNNSV
                     NVECLMNQVKTILVK"
     gene            complement(24655..26580)
                     /gene="mngA"
                     /locus_tag="ACGI59_000550"
     CDS             complement(24655..26580)
                     /gene="mngA"
                     /locus_tag="ACGI59_000550"
                     /EC_number="2.7.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HFG9738359.1"
                     /translation="MEFKKITNENLVFLKQKIKTKEDAIKFLARKLYDEGKLTSYEEY
                     LEAVMYRETLSPTGFERGLAIPHGKSKSVKEAAFAAMTLETPIENWESVDPNNKVELI
                     FLIAVPESEGGNTHIQILSELVTRLSNEDYMNMLLNSKTKKELFNNLDIKEKKEQIKE
                     EIRIQNESKKIILAITACPAGIAHTYMSAEALIKAGKEIGVDVYVEKQGANGMVDPHT
                     PDIIKRADAIIYATDVAPKNTERFEHLPNIKTSVAAPLKRAKEIIYEALEVAQKQGKG
                     DYIEKSSDISYCEKSSWKKDVKSAILTGVSHIIPLIVAGGMILAFAVLASQAFGLQEL
                     YNQEGSWLWQLRKLSGGMLGTLMVPVLSAYMSYSLAEKPGLGPGFAAGLAADLIGSGF
                     LGGMLGGLLAGYIVKFLKKNIPAKGTLAGFVSFWVYPVVGSLVVGLLMLFVVGKPVAA
                     LNNGLINWLNGMTGVNAILLGAIIGAMVSFDLGGPINKAAYTFAIGAIASGNFIPYAI
                     FASVKMVSAFGVTIATVLGRDLFSESEKEIGKQTWILGLAGITEGAIPFMIEDPIRVI
                     PSLVAGSAITGSIVAFFNIGLQVPGAGIFSLALLEGSMSGFIIASIWFFAAVLGAIIS
                     AILLIITRKQKNQRKIH"
     gene            complement(26710..27435)
                     /locus_tag="ACGI59_000551"
     CDS             complement(26710..27435)
                     /locus_tag="ACGI59_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFG9738360.1"
                     /translation="MEFYERAKKYEYKFTDTEDMIIEYMRKNKEEIVKLSIQSLADKF
                     FTVPNTIVRLCKKMEYDGFSQLKNNLKEELKNEDKEPKNGFESDIKKTLELIDYEKIN
                     KVADIIQKSRSIIVYGIGQNIPLCEILVKEIRGGHDNITYYSQRHEVLHDIGKLGEQD
                     MFFALSLSGETKEVIEAAKTAVNKGCKVVALTHICENQLQQIADISLYCYSTIKQVNG
                     YNISDKVPIMMIMRAIIEQYWLR"
     gene            complement(27698..28468)
                     /locus_tag="ACGI59_000552"
     CDS             complement(27698..28468)
                     /locus_tag="ACGI59_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HFG9738361.1"
                     /translation="MKKLVVRADDIGYSEGVTLGIISSYKNGIVTSAGIMVNMPFSRQ
                     AIEMVKNDDVCFGLHVNLVIGEPCCKEHIKIKNLLDEGGKFVSSKIRRTQLLTGKEVF
                     NYEEVYEEVCAQIETYKLYMSKLPEYIDIHGIEEDVSISAMCDAAKEYGIPSCPYYLD
                     SCISMPSKYPQNDFYKLNKSFVKLFEEKYINLDKDITLMVTHPGFIDYSLIKSSSLIQ
                     ERVYEHAMLCDKEVKMWLKRNNIELVDFKDIFKSNNYI"
     gene            complement(28470..29918)
                     /locus_tag="ACGI59_000553"
     CDS             complement(28470..29918)
                     /locus_tag="ACGI59_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFG9738362.1"
                     /translation="MKRFLNKMMQQLSLLGKTMLVPIAVMPAAGILGLLFGPNMLNVP
                     AISNISNIVFSNVDYLFLLGAASAYSKSKDKTSVMVAAVVAYMIFKSNLELLNDTLNA
                     GVFGGIIVGACIAWTYNKTYKVKVPAFLSFFAGEKCVITLSPVVALILSYIFSLLWIY
                     PQAAMNQFGFWLGGAGAIGIFLFLFCNRALIPTGLHQVLNAYILFEMGEYTTSTGEVI
                     RGEIPRFMAGDPNAGFFWTGFYIIMMFSIPAISYAIYKTAQDKKKQEVKGAMTAGALT
                     SFMATVTEPIEFSFLFASPKLYIIHSFYSGLGGVVLYLLGARLGSFNGASIIDYVLSF
                     SYGDKAWLVIPVGVVFFVLYYVTFKYIIIKDNVQTPGREVEIEIGDKVSEKEKNLKLS
                     HGNFSYMAKQIIKNCGGYENIVTLNNCMTRLRLEVKDATILNDDNIKKTGAKGVIKLS
                     NTSVQIIIGTDVVKVKDEMEMQLDELRKQADA"
     gene            30288..31010
                     /locus_tag="ACGI59_000554"
     CDS             30288..31010
                     /locus_tag="ACGI59_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFG9738363.1"
                     /translation="MAKNKFSEIYEVLKEEILDGKYTSNMMLPTELQLIERFSCSRNT
                     VRRAISQLNTEGYVQSIKGKGVVVLENSCSNDFFLNMHNFKGVESIVEDKKVNTATSV
                     LHFSKILIDNKLSKKTGFKVGSEVYYLHRLRYIDNIPKILDINYFLCSIVKDLDVSIA
                     QGSIYKYIEECLGTKIVSSRKIFKIEKATELELKTLPLNDYNCVGVIKNSVYTDDGKL
                     FEYTESKHTPETFVFMDVTQRY"
     gene            31207..32874
                     /locus_tag="ACGI59_000555"
     CDS             31207..32874
                     /locus_tag="ACGI59_000555"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFG9738364.1"
                     /translation="MKNWWKKATVYQIYPKSFKDSNNDGIGDINGIIEKLDYLYSLGV
                     DLLWLTPMYVSPQRDNGYDIEDYYNIDPKYGTMSDFEKLLKEAHKRDIKIMMDMVLNH
                     TSTEHKWFKESKKSKDNPYRDYYFWKDAKPDGSVPNNWISRFSGTAWKYDETTNQYYL
                     HLFEETQADLNWENEKVREECYKILEFWADKGIDGFRLDVVNLLSKTPGLPDDPITGP
                     KGDGRTHYADGPRIHEYLHNMNQKVFKPKNIVTVGEMSSTTPEECINYTRENREELSM
                     VFSFHHMKTDYKGGAKWTNEMFSLDKLKKAQSDFQYKMYEGKGWNALFYSNHDQPRAL
                     SRFGDDRFFRQESAKMLAITLFGLQGTTYIYQGEEIGMTNAYFTKIDEYDDAESKNAY
                     YHMIKSGIDEKEALLILQQKSRDNARTPMQWDNTIYKGFSNHKPWLKVNNDNISVENE
                     LNDSRSVFYTYQRLIKYRKQYDIFTEGTYRLLDENHKNLFVYEREYKNQNLLVISNFT
                     HDNVVYKLPETYLNKLNYTILETNYTRDIIENKMEIKPYESIMIYYK"
     gene            complement(32970..34289)
                     /locus_tag="ACGI59_000556"
     CDS             complement(32970..34289)
                     /locus_tag="ACGI59_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFG9738365.1"
                     /translation="MEKGIKLGFWSIVLLGINAIIGSGIFGLPGDAYTDIGPASILVL
                     VFCMLLAVSIALCFAEAGSWFDTDGGPYLYAKEAFGDFVGFEVGFMKWIVSMIAWATM
                     ANFFAVTLSSVWPQAAEPLTKNIIIGILVVGLGIINFMGMKQSKHLNNIMTIGKLLPI
                     VLFIAVGLFFIKGSNFTPFVIIQKGQSASSAFVAVSITLFYAFTGFESLAVAAKDMEN
                     PKKNVPKALVMVMFVVSVIYMLILGISIGVLGNGLAGSATPVADAAIKMLGPIGGYII
                     TIGTIVSVGGINIASSIFTPRSAAALVEQGLMPKSIRKTNKNGAPYIAIIVSVIGTLL
                     IAWSGSFTTLSQISVVSRFIQYIPTCLAVLILRKKYAGKDVNFRIPGGAIIPIFAVII
                     SILLLIKAGIDEPMKIVWGLGGMIIVVPIYFYMTKVYSKKYNDVEVK"
     gene            complement(34330..35076)
                     /locus_tag="ACGI59_000557"
     CDS             complement(34330..35076)
                     /locus_tag="ACGI59_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HFG9738366.1"
                     /translation="MKKLAKKKYPIIGISGNLLIDEGGMFPGYERAYVNNDYIQSVVM
                     CKAIPYIVPIVYDDEIIKEQVSNIDALILSGGQDVNPLIWKEEPHNKLGAISPKRDSF
                     DMKLLKHALDMKKPVLGICRGEQIINVTEGGSLYQDLSLIEGAYIKHNQQHLSNVPTH
                     TVQIKEGTKLYEILGEKEVLVNSFHHLVVNKVAPGYIVSATSKDGLIEAIEKEGSEFV
                     IGIQWHPEMMTRDYDNMKKIFMAIVKEASK"
     gene            complement(35320..37329)
                     /locus_tag="ACGI59_000558"
     CDS             complement(35320..37329)
                     /locus_tag="ACGI59_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFG9738367.1"
                     /translation="MKTIAVVTDGLSKLECFLNKNLEMIFKDKVMIKNYYLNKITEKE
                     LIKDDIILVMIDDRVLKIKKYVEDVSKIITINRSIKQKDIYKLFSLPEGIDVLVVNDN
                     KHTVLETISNLYNIGINHLNFIPYNPNEEYRNIKIAITPGEASLVPKYIEEVIDLGHR
                     YIDSSTFIQIIAKLKLDDKEITERLIKYTDEIISLYSGINTKYKELTIKADELNSIIN
                     LSNLGMAMVSDKGNIIICNNSLGDILDIQSNIIGKNIDELENENIKKIFSLSKAHDEV
                     IKFNNKYLNVNKYNIESFGRVTGYYFCIQEITYIRKLEQNLSKKLREKGQVARYTFDD
                     IKTVSTKMERTKKLGMKIAESDYTILITGESGTGKELMAQSIHNASPRHSQPFIAINC
                     AAMPENLLESELFGYEEGAFTGALKGGKKGLFEQANNGTIFLDEIGDMPIYLQSKLLR
                     VLQENQVMRVGGENVIDIDVRVIAATNKNLLEMIKGDRFRADLYYRINVLPINIPPLR
                     ERKEDIEVMLKHFMKRKRAISQDVQDIINAYEWPGNIRELKNTAMYINIMSSEDVILI
                     NDLPHNLLNITEDFSKEISILKDRTSIEKVKLVMECLKNANNLNKNIGRNGIVNSINE
                     LGYNITEGEVRGILNMLKELDLIICESGRKGSELNRRGKNILNSI"
     gene            complement(37767..39200)
                     /locus_tag="ACGI59_000559"
     CDS             complement(37767..39200)
                     /locus_tag="ACGI59_000559"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFG9738368.1"
                     /translation="MALRKDFLWGGATAANQCEGAWNLDGKGPSCSDMCTGGSQKVSK
                     RITRVIEKGTFYPSHEAIDFYHRYKEDIALFAEMGFKVFRFSIAWTRIFPTGMEKEPN
                     EAGLKFYENVIDECLKYNIEPLITISHYEIPFAITEKYNGWVSRDVIDLYMNYCETIF
                     KRYKGKVKYWLTFNEINSATMPMGGYLSQGILNEGTTDFINQVDIPQLRFQGLHHQFV
                     ASAKAVKLAHEIDSNYQVGCMQIFATMYPYTCNPDDAVKTQRDSRVMNYFCGDVQVRG
                     EYPTYMNRYFGENNIEIKMEKGDLEILKEGCVDFYTFSYYMSTCVSSDSKEDNTSGNI
                     LGGVKNPYLKSSEWGWQIDPEGLRYALNEIYDRYRIPMMVVENGLGAYDKKESNGVIN
                     DDYRIEYLKAHIEQMKEAVEDGVDLFGYTPWGCIDLVSASTGEMAKRYGFIYVDKYDD
                     GSGDLSRIKKKSFDWYKQVIESNGERL"
     gene            complement(39287..41203)
                     /locus_tag="ACGI59_000560"
     CDS             complement(39287..41203)
                     /locus_tag="ACGI59_000560"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFG9738369.1"
                     /translation="MRKYEQLSKEIIANVGGKDNISSLSHCITRLRFKLKDESIANDD
                     VLKKMDGIVTVMKSAGQYQVVIGNHVPDVYEVVCDLAGLGGDTIDNSDSDAPKGLLNK
                     FIDIISGVFQPILGVLCAAGMIKGFNAVFTALGLYGETDGAYIMLNAIGDAMFQYMPI
                     ILGYTAAKKFKLKPFIGMLIGAVLCYSGIQLDTLASTGQPLYNLFTGTIFESPVYITF
                     LGLPVIAMNYTSTVVPVILIVLLAAKLEKVFAKIIPDVVKNFFVPMAVLLVSLPLGFM
                     VIGPIATYASNIVGNGFLALFNFSPILCGALTGLLWQVLVIFGLHWGLVPIAMSNLMT
                     MGFDTILVGAFVPSFAQTAVVAAMYFKLKDKKIKELCIPAVISGICGVTEPAIYGITL
                     PRKTPFIYSCVGGGVAGAVMGIMNVKSYVMGGLGIFGIPNYINPTTGDMKGVYATIIA
                     IIVAIVVGFVLTLFFWKDDAIVEISKDDNGQGVIEKNKREVVYSPIKGEVKALEQIQD
                     AAFSTGILGKGVAITPKEGKVVAPFDGTVMTLFPTKHAIGIISDNGCELLIHIGLNTI
                     QLEGKYFKSFVNQGDKIKKGQILITFDIESISKEGYCLETPVVVTNYSDYVDILENTQ
                     KHVNNSQELITVLA"
     gene            complement(41277..42137)
                     /gene="licT"
                     /locus_tag="ACGI59_000561"
     CDS             complement(41277..42137)
                     /gene="licT"
                     /locus_tag="ACGI59_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HFG9738370.1"
                     /translation="MIIEKILNNNVIITTDENHKEIVVMGRGLAYKKRTGEHISKDKI
                     DKIFKLSDPNISDKFKELIADIPIRYMELSDEIILYAKEKLGKRLNDSIYISLTDHMY
                     TAIERAREGVSVKNVLLWDIKRFYKSEFKIGLEALDYIEKKFEIRLSEDEAGFIALHI
                     VNAQMDQSIKTIYEITQIIQEISNMVKYHYRIVFDEDSVYYHRFITHLKYFAERIVSN
                     NLHENNEDDLLNVIKVKYKNAYKFIEKLDEFIHKKYNYDLTDEEKLYLTIHVERVVSK
                     SIKSDINPNN"
     gene            complement(42488..43675)
                     /locus_tag="ACGI59_000562"
     CDS             complement(42488..43675)
                     /locus_tag="ACGI59_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFG9738371.1"
                     /translation="MIESIKKRAYEIENELGKEIEEVCNFIFNNPELGEEEYISSKYL
                     VEKMKEYGFDTVYPYCNMETAFRAELGDNDGPTIAFLAEYDALPGYGENKEPAHACGH
                     NWIAASTLGACIVLSKLKENFKGKIVLIGTPAEETTGGKCDLVKAGAFDDVDVSYQMH
                     IEAFNNINCKALAIDSLEFSFEGVSAHAASHPHMGVNALDAVQLTFAGINALRQHVKS
                     DVRIHGIVSNGGEAPNIVPEKAACKFFVRAAERSYLDEVTKKVINCAKGAELMTGAKL
                     SYRYFENSFDNIINNKVLQKITKNNLIEAGITDILEGKDGPVGSTDIGNVSQVCPTMY
                     TEIALDISPMVYVHEKEFLNYANSEEAYDKLHKAVKAMVGCALEIYLEDGLLDEIKKN
                     HLN"
     gene            complement(43689..45080)
                     /locus_tag="ACGI59_000563"
     CDS             complement(43689..45080)
                     /locus_tag="ACGI59_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFG9738372.1"
                     /translation="MSSLKKKKFTMPQTLTIIFLLIVLMAILTWIVPSGNFERVDIDG
                     RSVVVAGTYEKAPSNPQGITDVFTAPINGFIDAAEVVGFVLIVGGAFGIVNKTGAIEA
                     VIAHTVNKMKKFQFLIIPISMILFGLGGTTFGMSEETLPFYMIFIPLMTSMGYDSLTA
                     VATVFIGATAGFGAATTNPFSVGIAQALSQIVPGSGIEFRVVMFIIYMAISIGFVMMY
                     ANKVKKDPKKSLVHDISLNQELMVNSDTNIKEFTKREAMVIAIFTIGMAIMIYGVLRL
                     EWYITEIAMIFTAIGIISGIASGLKQDEIVNSFISGAGDLITAGLCIAFARGIVIIAE
                     NGFIIDTILNSAANLLNGLPKTIFINLTFLIEGLIAFLIPSASGLASLTIPVLAPLGD
                     LVDVSRQMIVTAYQFGIGVTNLITPTSGVLMGALAVANIPWSKWVRFVIPLMVVLTIL
                     VMAFLTIGLYLGF"
     regulatory      complement(45224..45403)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            complement(45675..47327)
                     /locus_tag="ACGI59_000564"
     CDS             complement(45675..47327)
                     /locus_tag="ACGI59_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFG9738373.1"
                     /translation="MKDKLQVLSGAMMIPVILLVVAGVFIGLGSAFANMENVSALGLS
                     GLIKEGSFINVFFKVINDLGFMVMRYLPIFFVTGIAFGLSKKEKGWGALGGIVLFIGM
                     HTVISTLLAANGINSDTVTAEAFMASGLGEAEAYSRSSLYGSFLGIFSYDCSIFGAII
                     AGFVASAVHNKFVDIELPSALSFFSGPRFSMIMMFIASIVLGTAMYFIWPPVGLALSK
                     LGNWIGSSGLLGTFTFGAMDKALLPFGIHHLIAFPIEYTRVGGTMEVGGAIYEGVNNI
                     RLAQMGDPDTLSYITRNFTTGRLLIHFAILPAAALAMYKLADNANKKKAISILIPAIV
                     TAMLVGVTEPIEYTFLFVAPLLYFVAYVPLAGLTYVFTEMFNVSIMGESFRNMFPNLL
                     QPQKVDALPLLFLIPIFFIVTYIIFTWAIKKFDIKTPGRSSEEVKLYSKKEYRERQSV
                     DAGKTAYNNAVEEEVDDKDTKLVHSIIEGLGGSNNIKNVTNCATRLRVELNSIDGFYE
                     DGFWVNELGASGVVKKKNSVQVIFGPRVITIASKLKAVLGVD"
     gene            complement(47347..48750)
                     /locus_tag="ACGI59_000565"
     CDS             complement(47347..48750)
                     /locus_tag="ACGI59_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFG9738374.1"
                     /translation="MIVNKELIIDYLKSNQENIINDIRSLVEIPSIRDESTTDINQPF
                     GIEIRNAFDKLIQIAKDKDFVVKDFDGYAIHIEYGEGEEVVGVLNHIDVVPIYNKELW
                     KSKPFKVCQKDNYLYGRGVNDNKGPLIGILYALLFLRELNEKPKRKIRLIVGGAEETT
                     WECMEHYFSVNEQPKFAFSPDGNFPIVNGEKGILYFNLRKKIDKDKFRNHNLVDIKSN
                     KEDGFVCDKIEAVFKTNDKKDLVESLAYYTEIEELDEGKVLVRYTGERALSRNPHRSY
                     NCAFSLAKDLEKIKKLNDKGVIIRDILNSYFTDDNHGKKLGMYKEDVDMGVSTICIMS
                     IFLEKNELNMKIDFRYPKGISWEFITNRINEIGKKENLIVDIYKDLKLLYVKPDSELI
                     NKLSNAYKQGFGKEAELFTKGAASYARVLKNGVAFGPTIEGDNPNSHQANENISIDTL
                     YKAIEVYIYALYSLAFQ"
     gene            complement(49014..49505)
                     /locus_tag="ACGI59_000566"
     CDS             complement(49014..49505)
                     /locus_tag="ACGI59_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFG9738375.1"
                     /translation="MFNIFKKKENKIEKIYSPFKGNLVNLEDVPDAVFAQKMMGDGVA
                     IIPSEGKLYSPVDGEIVNVFDTKHAIMIKSNDNTNILIHVGLETMSLEGKPFDVKVKA
                     GDKVKAKDLIMEVDLDFITSNDLNIITPVVIMDDADSVKRTVKDRIEPGNVDINSIIM
                     EVE"
     gene            complement(49531..50343)
                     /locus_tag="ACGI59_000567"
     CDS             complement(49531..50343)
                     /locus_tag="ACGI59_000567"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFG9738376.1"
                     /translation="MRYKLICTDMDGTLMGKGFEVSEENIKALKEAMEKGIKVALVTG
                     RPYNAMKYFTSVLGDDIYIISTNGTYFKLLGYEYKKVLSKEALKKIYTIGEKYNLNKH
                     FKGCKIVISNNEIGEEHPYRLINSKNKEEDRIEIIENASCETLLEKADNEILKCILFS
                     ENVDSLREAKEEFKKQEDLEVVSSGKINFEVMSKGTSKGIAVKKFCDILGIDSKEVIC
                     IGDNENDISMIKFAGLGIAMGNATDEVKSMADFVTDTNVNDGVAKALRKILS"
     gene            complement(50356..51309)
                     /locus_tag="ACGI59_000568"
                     /pseudo
     CDS             complement(50356..51309)
                     /locus_tag="ACGI59_000568"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
     gene            complement(51433..>52372)
                     /gene="tnpB"
                     /locus_tag="ACGI59_000569"
     CDS             complement(51433..>52372)
                     /gene="tnpB"
                     /locus_tag="ACGI59_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS200/IS605 family element RNA-guided
                     endonuclease TnpB"
                     /protein_id="HFG9738377.1"
                     /translation="TNLKKELNWLKEPDKFSLQNALKDLDNAYKKFFKEKAGFPKFKS
                     KKINRFSYKTNFTNGNIMYCGQYIKLPKLGMVKIRDKQVPQGRILNATISKEPSGRYY
                     VSLCCTDVDIEAFENTNNHIGLDLGIKEFCISSCGEFIENPKYLKKSLNKLAKLQREL
                     SRKTIGSLNRNKARLKVARLQEHIANQRNDFLQKLSTKLIKENDIICIEDLQVKNMIK
                     NRKLSRSISDVSWSEFIRQLEYKANWHGRQIVKVGKFFASSQICNKCGYKNEEVKDLN
                     IREWICPSCNETHDRDINASINILKEGLRLITIQNK"
BASE COUNT        16721 a         9609 c         5542 g        20500 t
ORIGIN      
        1 cttcatttaa ctctatatca aactcattaa gtaaaaatct aattatattt aatttagcct
       61 ctgtatttaa atttgtttct aaatatatat tttctgatat tttaatacct tttcttaatt
      121 tatatgcatc tctagactta atattatcta taaaatctct tttataagtt ttgtctacaa
      181 tactgttaaa ccttacagaa tccaaatcat ataaagtaag agcaacttgt tcataaaatt
      241 ctgtccaatt tttagcctta tatgactctc ctaatatact aaatgatact atactttcat
      301 ttgtaaaatt aacatcatca tcctctaacg taaatatatt ttcactgtta cttggaggtt
      361 catatgtaac acttggatat ttccaaattt ctacagctct atctagtaat atcttagttc
      421 tattttttat ttcttcttca ttccatttat ctatagatga aatatattta tttagataaa
      481 gcctactatc tttaaatcca tcttccattt ctttcttttc ttcaaatgtt ttattactta
      541 gttttgaatt atatcctgtc aaagttaaat ttccaattgt atgtaaatat ttatcatgta
      601 tctccttata gttatctcct agtgattcct tccatttatt tgttaatgtc tgtggcatta
      661 tatgctctat atttagcata ttattattga ttaaattttc taaatcaaca atttctttat
      721 tactataatt ctctaacctc tcaagtatat ataacttatt ctttgactta gtattatata
      781 tatctttagt ttcaaaagca tttgaaaatt cactgtcagt aggaaatctt tgagatgatt
      841 tcttatttat aaaaatatat cttaatatat tagaatagtt ttctttataa tcactaaatt
      901 ttttaatttc ccttcctaag ttcataaata ctttattcag tgcattcgga ggtacatcac
      961 atactattct tcttaatata aaacattcta ctaaattaag ggattctaca agtacttttt
     1021 tatttattat accttcatta taatcatcaa ataattctaa tataaacgga tatgatacaa
     1081 atatttcaag tttgtttatc cttttcaaaa ctttgtttac atcattatca tcagtacatg
     1141 aataagatat ctctctataa tagttagaaa atttaagtaa atcttttaat aaaatttcta
     1201 tatctatacc attatctgat acataccttt taaaactaaa atatacttta tttttattcg
     1261 gtatacttct ctcctttata gtcaaatagt ctcttataaa actacttacg ttgctacttg
     1321 tatttttttc aattttatac caataattat tataaaattc ctcttgtaac ttagagcttc
     1381 tacccattaa cacaaagttt cttactctat cagcttcact taaatctaag ccagttgaat
     1441 ttagactttc aaagattaat tgagggtcat cttcaccatt ttttaactct atttctacta
     1501 tagttaattt ctgtatagca tcaaacaaat catctattga tatattatct tcttttattc
     1561 tattataaaa atataaataa tttgaaacta tattagaatt tctatctgta ttcatttctt
     1621 cttcaaatag ttcatagtac gcttttctat cttctgctat tggttttaac tttatttttc
     1681 catctttatc tcttgagtgc ttgtttataa gataacactc cttaatctgt tcttctgttg
     1741 taataattcc ttttgattcc ccaaactctt ttattatttg acacatggca atcaataata
     1801 ttgacacagt agtcactctt tgttgtccat ctattattaa atattctttt cccatactat
     1861 atatagatac aactgaccct aagaaatgag ttctaaatcc agaatctatt atatcctcta
     1921 aatcatcaaa caatctttta cattgttctt tcttccagtt ataatttctt tgatatacag
     1981 gtactacaaa acttttgttt gacccctcta aataactaag aaattcaatt tctcctgctt
     2041 tcatattaac tccccctata agttaaataa attccattca taactccatg tatctatata
     2101 ttatatcaaa attttgactc ataaatgtat atgaaagcct tattatatac ctttttatac
     2161 ctgtttttat tttattaaag taaatacatg attattctat aaaatataca aatacttaat
     2221 tttaaaaatt attatttaat cttcttcatg agtaattcat agttatttat caattaaaaa
     2281 gagatattgc ataattattc aatacctctc tttgtatatt tacattatcc atatctagtt
     2341 atctatttca acccaatatt agactaccta ctaataaatt tattctctac atcattaaca
     2401 ataattttgc actaaataac atcattagcc tatctctgat gcattttctt taattttatt
     2461 tatagtttta atcaatttat ttctctcttc aatatgattt tcaaataaat caattatact
     2521 tccaacagtt ctaaatgggt cttccatcaa cactttgtta tcctctataa atccattctt
     2581 gactacataa tcgactataa gtttaacaaa atgtatttgc ttagtattaa agctcttatt
     2641 atttaaaaac tctgaaaaag cttcatttgc agcttctcta tccaatccca ctagtcttct
     2701 cactaattga cttactggag tatctccaaa ctccttctta taatcatcac aattacctaa
     2761 ctgttttaag agtatttcct ccaacgtttc aacatcttgc tttgttatct ttttattatt
     2821 tcttaattta aatattgcta attcatcttt atggctattt aaataatact ctaccttctt
     2881 tttataattc ttaagattgt ttgcattata tacagaatca tttctatctt ctgctacaat
     2941 catatcttca aaacttgtat aatatatctt ttgactagac ttttcaagat acttaataag
     3001 ctctcttaat gatatacgta cttcttctac ctcaaatata tctgaatctt cccaaaaatt
     3061 ctctgtcatt gctttttcaa tagtatattt ttgttgctga atctgtggta tagtcccaag
     3121 ttttgaaagt ttttctgctg tagccattac atgccttata cctttacttg cattattatt
     3181 ttgtaagtat gcaagttgta tattatacat aactacatca aatcttttag ccaattcatc
     3241 atcactatat gaagttatga gtggtgatat atattctctt atctcatttt gtgctactgc
     3301 tccaatattg ctccaaacat tttcattttt atacttatgt acataactta aattcatcct
     3361 aactatataa ctatcctcat ttaaagcttt tacagaagtt ataagttctt ctaacaattc
     3421 ttttctaagt tctgcatact caccttctat atatttaata tcttgaagtt ctttaaccat
     3481 acttaccttt atattaaata atttttcagt aagtgtttct gctttatttc cctcaaatcc
     3541 tttaggatta aatttaaaaa attcaaaatt attacaaaaa tcaaatatta agaatctctc
     3601 cttatccata tcaacaccta ataaatctgg acataatcta gttcctctac ctatcatttg
     3661 ccaaaactta gtcttacttc tcactttctt gaaaaaaaca agatttagta tttcaggtat
     3721 gtcaattcct gtatcaagca tgtctactga aactgctatt tgaggtaatt tattcttatc
     3781 agaaaaatca tctattatag aatctacata atttatacta tagtcaactg cttttacaaa
     3841 gttcccacca tactttggat acagcttatt aaatctttca actattgccc tagcatgctt
     3901 tgtattcttt gcaaaaatta tagtcttacc aatcttttct tctccttcta ttttcaaccc
     3961 ctctgtcatt agcttattta aaactaaatc tattgtgtca gaattaaata gccactcatt
     4021 tactgcatta ttccctattt cgtctccaat actctcatca tcattaaatg tattttcata
     4081 ttcttccttc tcttcatcag aaagctcatc atacttaatt ccatcttcca taatcttagt
     4141 cttaaactca atactagtat aatctactaa atatccatcc tcgactgcct tattatactc
     4201 ataagcatat gtaggtactc cattttccat gtcaaaaact gaataagtat ttttatctat
     4261 ctcatccttt ggtgtagcag taagacccat taagtaagaa tcaaaataat caaagattga
     4321 cttatacttt ttataaatac ttctatgcga ttcatctatt ataattaaat caaaatgacc
     4381 aggtgtaaac aatctctttc catccttaga cttcgtatca tcaattgcat tcatcattgt
     4441 tggatatgta gaaaatatca tcctagcatc ttctgggctg tctttgctat ccaataaatt
     4501 acataacgcc aagttaggta atagcttagt aaaacttttt ttagcttgct ttaccaatgc
     4561 ttttctatct gctagaaata atatattttt aatccaatta tgtcttgaaa gtacatcaac
     4621 tatagatata gcaattcttg tttttcctgt accagtagca cataccaaaa gagttttgcg
     4681 ttgcttattt tcaagggcct cacatacagc ccgaatagct tctttttgat aatatctatt
     4741 ggatatttca tcatttattg ttacagaact taaagacttc ttactctttc ttctatccat
     4801 cataagttgt aattctgatt ttttataaaa tccataaact tttctatctg aatagtcatc
     4861 ccaaatatac atatcaaaac cattagtcaa aaatattact ggtctttgac tatatctctt
     4921 ttccaaacaa tctgcatata atttagcttg ttgttgacca atttttgggt ctttgctcgt
     4981 tcttttcgcc tcaactactg ctaatggctt accattttca ccataaagaa cataatctac
     5041 atatcctaca ccactttcat taggcatacc ttgtacttct atttcctccc ctatatcttt
     5101 tccaaattcc caacctgcaa gctttaattc tatatttata tatctttttc ttgtctcaaa
     5161 ttcgcttact tcgtctatct tgaaatcata gtttacagta ttaacttttc tcttttcagt
     5221 tagtgacttt cttaattcct cattttcttt tattacttct tcaagctttt tatcttttga
     5281 acttaatctc tcgtaaagat ttttaagttc gtctggtctc gtccttttct cttctccatg
     5341 aagtaataaa tcttcattaa agtcttctgc tgtatattca tctgaataac aatagtctat
     5401 ccaactaaca aattgatgca aattgttaag tgaaagtatt gcttctcctc tctctatgtt
     5461 cgcactagta tgtacagata aattacctaa cttaactata tattttaata aaggtaacat
     5521 atcatagtct ataagttcta taaaactatt attatgaatc aacgtactga gattatcttg
     5581 ataaggcaat actaaatcac tatcattagc atatagccat tttactgcaa gctctaaagc
     5641 tcttcttgtt atagttgcac aagtagatgg actaactagt atactttttt ctgcttctaa
     5701 gcaagcagta ttaaaactat tgaaattttt cttcttttgt aaaaaatcaa aattacccaa
     5761 ggcttttatc ccccttttgt cccttagtta aaataaatct ctctaaaaac tatttcttac
     5821 agctgtttct tatactaaca aatacttctt cattttctgt aaacaattcg tacatatctt
     5881 caaactgtgg tatatcatat ccatgagaat catcatttct caattttcta agtgtttcta
     5941 taactataga agaattttta ggtaataaat tgtgatactc acaagctaaa aggtagtcat
     6001 aaaagttttt agattttatg tttaaatcaa ttgatttata atacaacatt gcttttgtta
     6061 attctagaaa tgataccata tagcttttaa agccttcttt tatgtatcta gcccataact
     6121 caggcatttc ttccttatca tataagattt tgaagttttg caggtcatac aagcaagaac
     6181 caaaatccct atttacttct tctattgagt tccaagataa tttgtttcta attaccatac
     6241 atgtccctcc aatctaagat atctattaca tcactgtttt caaaatagaa tttatttata
     6301 taatccttaa aatctttaga ttcataaata acttcacctt ctaaaatagc ttttttaagt
     6361 gctattgtat aattatctgt aacaattttt aggtctacat caatgtctaa tatatttctt
     6421 agagcttctg tcttagttgc aagttctgta attggtactt ttttgatggg aatccaagct
     6481 atgtctatat cactttctga tgtatgttta tctgttccat aactaccaaa tattatgatt
     6541 gctagagtat tttcttttgg aaatacattt attattgagt ttttaatctc atttggtata
     6601 tttctcattt aatcacctct tgtttattag gtaatattgt ttagttaaat aattctcctt
     6661 tgaaagcttt ttgcattaat gaattaaagt tatcttctaa ctcttttaag ttggtttcta
     6721 tttttaattt taatttatcc acttgcttaa caaactcaga aaactgattt tgaaattcaa
     6781 ttggaggaac cattattaaa tattcaccta acttactaac tgataactca atttgattcg
     6841 ttgaaccatt aacacatttt ctatatattg tgtcttgtat atttgtaaat tcaaataacg
     6901 ctgcaaaata atctgaattt aagttatttg aaatacttct taaaactgta acatgactat
     6961 ctgctatata ttcagaatta tcaattttgt taaattttac acatcttcct aatgtcccag
     7021 ttccagtaga atttattaat atatcatttt gtttcaaaaa tatatcttct atcttctctt
     7081 ttaagttttc atcataatat ttacaatttt ctaccttcaa atttctccag tatatacatt
     7141 tttgatttat aacttttata tttgattgct ctacatattt aggagataca cctcttttta
     7201 aatatataga aatctcttta atcttcttca tatcaaattt ttttgtatta ttaattggct
     7261 ccccaaacat ctcaataaat cttgatttaa ctaattcatc caatgcttct atttgctctt
     7321 ttcttttatt tattaactct tgagctttat ttaaaacttc taccatttta ttttgtatac
     7381 aaattgtgaa atatggtatt tttatatcat ttaacttgct tttatttaaa gttgcaccca
     7441 taactgctct atcagtatta ttaaaattta aagttctcaa agcataatat aaatattcag
     7501 atgttataag ttcattattt tttatttgaa agttagcaat tgcttcattt gtaaacatgt
     7561 cttcttttaa tatggcaact ctacctatag acaacttaaa actcattaca acggtatttt
     7621 taggtactaa tttcatatta gctttttcaa cagctaaatc tgttatctct tcttttgatt
     7681 ttgatatata tttttctttc aaatcagaaa ttgataacca tctattacca cttccccaat
     7741 aatcagaagt atttctagat ggtgttttgc caatatttat atagcacaat tcatttaatt
     7801 ttatatactc cataccctat acctctaaca ttctttctaa ttcttctaat ccaacagcta
     7861 tttccttctc taactcccta actctaccta taatcacact aggagcatca taaactactt
     7921 cctcatacac tacctcttta tacttattaa tagataaatc atacccattc tctcttatct
     7981 cttccacatc aacaaaaaag ctttgttctg ttctttttct atctacttcc ccatcaagat
     8041 tatcaaatct cttaataata tcctctatat cactatcctt tatctcattt ctcttatcat
     8101 ccaaagaata accatcagct ttcatatcat agaaccatac cttatcagtt ccacctgtcc
     8161 ctgttttagt aaatatcatt atagctgtag atactcctgc ataaggctta aacactccac
     8221 ttggcattga tattatagct tccaatttat gattatcaac tatttctttt cttatcgatt
     8281 tatgtccacc tgttgaacca aacaatactc catctggcac tatacatgcg catcttccac
     8341 caatttttaa aactcttaaa aaaagtgcca agaataataa ctctgttttc tttgtcttag
     8401 tcacttttaa taaatctgct gacactgctt cataatctaa acttccctta aaaggaggat
     8461 ttgctaacac caatgtaaac ttatctttat ctgtattaac ctccgataaa gaatccttat
     8521 actctatatt aggattgtca acgccatgca acatcatatt catagcacct attctaagca
     8581 tagttctatc catatcaaat ccatagaaca taccattatt aaaatgctcc ttcagccctt
     8641 gcactaagaa aagacttgaa tgattatccc ttaagtattg ttgtgatgat actaaaaaac
     8701 ctgctgaacc tgctgccggg tctactataa tatcttctgg tgttggttta ataagtctta
     8761 ctatcatatc tattatgtgt cttggagtcc taaattgacc attagtacct gctgtagcca
     8821 ctttggataa taaatactca tacaaatctc cctttgtatc tctatcctta cttaattcta
     8881 aattactaat accatcaact attttagata acataagagg cgtaggaatt ttaaatattg
     8941 catctcccat gtactttgca taagctgagt ctttatttcc atgtaatttt tttatgaaag
     9001 gaaacacttc attttgtact atttcataca ttttttctga atttccttca ttagaaaact
     9061 tactccatct taaatgttga tgttcatcat caaatatcct ttcaaaagaa actcccaaaa
     9121 gagttgcttc tgcttcattt tttgtttcta cttcatccaa tcctttaata aataaaagat
     9181 atgtaaattg ttctataact tctaatggat tagttaatcc accagtccaa aatgtctccc
     9241 aaatcttgtc tactttgctc cttaattctc ctgtaatcat atatgacttt ccctccagtg
     9301 ttcataaaat aatttaaata tatattttag tcgtacgcct taataattag tgtcttatat
     9361 taaattcaaa attttactac acagttatta aattattttt cctatttaca tgttttagtt
     9421 aaaaaactaa tgcaatactc ttattttatg attattatac atcccataca aaattatttt
     9481 atcatatatc cagcatagaa gtcatctacc tggataactc ttaccatatt ataacttttt
     9541 aatattaatt taaacttaga aagaaatttt ttatttaatt atatcatctt ccttatttca
     9601 aatttctcaa ttacaatctc aatcaaaaaa taaaataaga tgctgtctca aaatagatat
     9661 aatcttcttt tttattttcg gaatgatgaa aatatattta atttcataca aaaaagagtg
     9721 cctcctgaac taaaaagttc attttgagac accccttaat ttaatcttac ttattctctc
     9781 cctttattat taacacttca ctatctctat tgcttttcct acacatccac aaacttccat
     9841 cctactctta acaaaatctt taactgactc taaacctttt ttaccatatt tcttagggtc
     9901 aattacctct gggtcagaat taagcacatc cttaacccca ttgctataag caatccttaa
     9961 ttcagttgca taattaacct tgcaaatccc tctttttata gattccttaa taacttcatc
    10021 tggtattcca gacccaccat gtaatactaa tggaactgat acaacatctc taatttcaga
    10081 taatctatct aaatcaagct ttggctctcc cttatataat ccatgtgccg tacctattgc
    10141 tactgccaaa gatgataccc ctgttctctc tacaaactct tttgcttcta atgggtctgt
    10201 atatgctccc ccatcaccac catctaaatc gtcttcctta cctccaactt tacctaattc
    10261 agcttcaaca ggtatatctg aaggcataca tgcatctaca acagatttag atattgaaat
    10321 attatcttcg taactttcat gagaaccatc aatcataata gaagtatatc caactctaag
    10381 tgcctgcata gctagtccaa agcttgaacc atggtctaag tgcattgcta ctggaacact
    10441 tgccttctct gatgctacct ttacattcgc taaataataa tctagccctg catactttat
    10501 tgttgaaggt gttgtttgca atattactgg tgatttaagt tcttcagcag cctctattac
    10561 tgccataacc atctccatat tttcaacatt aaatgcacct actgcataat gtccttcttg
    10621 tgcatctaat aatatttgtt tagttgtcac taatgccata atttctcctc ctatatgttt
    10681 atactgattt gatttcttcg ttatttaatt ttgctactat ttctctactt gttttgctat
    10741 ttaataactc atttaaaaat tcttcattca ttaattttga agatagttga gataatgttt
    10801 ttaagtgtaa attatcacca ttatctgtag ctgctatcat aaatataata tgtgctaagc
    10861 ttccatcttc tgattcaaag tcaaaccctt cttttgatac acctactgct acacgtggta
    10921 cctttactgc atctgttttt gcatgtggta tcgctatccc cataccaaaa cctgtagatg
    10981 atatttcttc tcttttaagg atttcttctt taaacttctt tttatctaaa agaacactag
    11041 atttatcaag tattccaatc atttcatcaa ttacatctgt tttatttttt cccttaacat
    11101 ctaaaattac tgtttcttcg ctatataaag agatattatt agatacgttt tccgattctg
    11161 cactttcatt tttatgttca tcttttgtta catttgtatt ttctatagta tttttctcat
    11221 ttactacttt aggtgtaacc atcatataaa gtacagcacc tactaatgaa cctacaaata
    11281 tacaccctac ccataaaagt ggcttattga ctaatcctag aaccaaaaac cctccatgtg
    11341 gagctggtac ttctattttc attatataag ttaaaattga tgatattgat gatgcaatca
    11401 taagtactgg caacactttt aatggatttt ttgctgcaaa aggtattgcc ccctctgtta
    11461 tatgagtact acctagtata taattgatta atcctgctgc tctatcttct tctgtaaatc
    11521 tatttttaaa taaagtagat gctattgcta ttaccaatgg tggtgttata caagctgcag
    11581 atacacctgc cataaataaa taatttccct gtcctagaag catagttcca gttacatatg
    11641 ctgctttatt tactggacct cccatatcaa atgctgacat acatcctatt actattccca
    11701 aaattatagg gcttgaattt tgtaaccccg atagaaagtt catcatagca ttgttaagtg
    11761 ctgaacatgg cccaccaagt aacaacattg ctacacccac aagacctacg ctgacaatag
    11821 gaattataaa aatcgattta agaccttcta attgtcgtgg taattttctt aacaaatgta
    11881 caaatagttt aactaagtaa cctgctaaaa atcctgatat gattccacct aaaaaccccg
    11941 aacctgtatc aaatgatatc attcctccaa caagtcctgc taacatacca gctcttcccg
    12001 atatagattg agctataaat gctgccaata caggaaccat caagttcata gaatatccac
    12061 caacagattt taatgtagct gctatttgat tatattgttc tgaatttggg tcaaatgaat
    12121 aaatccccca taaaaatgat atagcagtaa gtactccacc tgctactact aaaggtaaca
    12181 tgtgagatac accattcatt aagtctttat acaacttttg accaaacgat aaatttcctt
    12241 cttctgcaat tttactgctt gataagtttc cttttctaat agctactttt ccatcaatac
    12301 atctttgtat aagttcatta gctttatgta taccttcttt gacaggtact tctattactg
    12361 gctttccatt gaatctgtcc atatcaacat ttttatcaca agcaactatt attgcatctg
    12421 ccatttcgat atcttttgct gttaacttat tttctacacc tatagctcca ttcgtttcaa
    12481 cttttatgtc acaccctaat tccctggctg aattttttaa tgcttcttct gccataaaag
    12541 tatgagctat tccagttgga caacctgtta cccctagaat cattttcttt cccattttta
    12601 taccaccttt ctgactctta tattttttat ataatcttcc acattctcca aattacctat
    12661 accattactt tcagccacac ttgctcctgt tgctgctgac ctttttaatg cttcttcaat
    12721 atgttctgga ttttccaacc atatacttaa aaaagcagct aatgcagaat caccagcaca
    12781 tgcagagctg actactgtaa ctggttgagc actagcataa taaattgatt ttccattata
    12841 aaaataagaa cctttttctc ctaaagttag caatatattt tgagcacctt ttaaatgtag
    12901 taatttaagt acatccttaa tatcttcttc atctcttaca ataattccaa atatatcttt
    12961 gatttcttca tcatttggtt ttatcaaata tggattatac tgtagtaact cctttaattt
    13021 tggtgaactt atatcgagta ttgtctttat acttttattt ttacaaattt taaatatatc
    13081 ctcatagtaa ttatcatcaa taccaggagg tagacttcca cttattgata agtaacttat
    13141 atctgctaat gactgtatct tatctagcat atctagtttt tgtttttcat ttacatatga
    13201 cccagagttt acaaatttaa attcatcact tccatcattt aaaaatatat ttatacgagt
    13261 tgtatcttct acccaagtag gcaatacgtc aaatcctctc ttttcgcttt cttcaactat
    13321 atattttcct gaaaatcctc caaaaaatcc taatatctta gattctatac cataatgacc
    13381 tagtacaaaa gttacattta atccctttcc attaggagaa tatacagcat ttgatgtacg
    13441 atttactaat tttctcttta tcccattagt agatatattc atatctattg caggatttgt
    13501 agttaatgta tatatcattg tcttccccct tgcttgtgct gtgtatgttt tcattataat
    13561 aaaaaagtct atgaaaatct tttcacaaat agaaaatcga ttttcataga cttttcaatt
    13621 taattttcag aattattata tctgaccata taatctataa ttatcctaga tataatatac
    13681 aagggcaatc tggaagatac ttcatattca gaaattgata tatgttcatg tttgtatcct
    13741 acaatatata aatccgaata ctttataagt tttgcacttt cactacaaca gcaagttata
    13801 accttagctc caactaaatt ggcattctgt gctgattcta caagctctat ggtatttcca
    13861 ttcaatgaaa atataattat aagttcgtct ttttttattg atttagattt tattttcatt
    13921 atgttggggt catttataaa aacaacatta aaatcaagta attgcaattt aagactaaac
    13981 tcttttgcta catattcagt aagccctctt gctaaaatat atatcttact agatttattt
    14041 atagttttta ttatattgat tagtgtagtt aaagatattt gttctataac tctctgtgct
    14101 tctataaagg atttatttat tatatccccc atattttgta tatcatcatt ttcatctttt
    14161 actgttaatc tatacctaag ttcattgaat ccatttaaac cacattttct aattgctctt
    14221 gaaacggttg ctggagatga atatgtttca tgagcaatct ctactataga taattctggt
    14281 aatattgatt cattttcatt tataaattta attatttcaa gttccgtctt cgttagattt
    14341 tcagaattac ttttgtttag ctcaattatc atatgttatc tccccttata taagttttat
    14401 ttgtctaaat ttatcgagta atataatata ttacctctta actacttatt tttatataca
    14461 attaagattt agtcaatgtc tatatatgta aatttataat ataataattt tttgcttaaa
    14521 gtttattatg ttatcttata gcattttaaa ataaaaaaga attgtacctc taccaaaatt
    14581 tggatgaagt acaatagata ttcctcatac tgatatgttt atgattaaaa gtataaatat
    14641 aaatttcgct atgaatcata taattaataa atcattttat ttacaattat aacccccctt
    14701 ataaaactat attaataata tatacagtat ttataagggg ggttgtttat atttaaaaac
    14761 tatttacttt tctcttaatt cttctccatt agttcttatt acattttgat accaataaaa
    14821 actatctttt ttataacgtt ctttacttcc atttccattg ttatctatat ccacatatat
    14881 aaatccatat cttttttcca tttcttgaga tgaacaactt actatatcta ttggcgacca
    14941 catacaataa gctaatattt cggttccatc agctatagct tcacctattt gttctatatg
    15001 ttttcctaaa taatctattc tgtaactatc atgaactttt ccatcttcaa atttatcatg
    15061 cattccaaat ccattttcag ctatcattat tggcttttgg tatctatcat aatattgaga
    15121 taaagtattg tataatcctt ttgggtcaat tgcccacccc caaggatttg cttttaaata
    15181 tggattcttt atattgtcag ttggattcat ggtgttttta cttgctgata ctgttgtaga
    15241 ataatagtat gataaagcta gaaaatccat agtattttct tttaagattt tatcatcacc
    15301 ttcctccata actatattta tgttattttc tttgaaaaat ctcttaatat agactggata
    15361 ctctccccta aaacttacat cagtgaaaaa atattgtagc ctatttcttt ttaaagcaaa
    15421 tactacattt tcagggtcac aatcttctgg ataagatgta caatctgcca tcatcatacc
    15481 tatcatcata tctgaattta tttcttttcc aagcttctta gtcaaagctg tagctatgaa
    15541 ttgatgatga atagcttgat atctagtctc ttctacatta tcaacttggt ctacacaaaa
    15601 tgcaactgaa ttaaatgatt cataatgtat taaatttatt tgatttataa ctatccaata
    15661 tttaacttta tttttatatc tatttagtat taattctgca tatctaacaa agaaatctac
    15721 cacttttcta ttagaccatc ctttatattt taatactaaa ttcaaaggag tctcatagtg
    15781 aagtagtgtt acaataggtt ccataccatt atctattact tcatttataa gattatcata
    15841 aaactttaat ccctcttcat taggctctaa atcatctcca tttggaaata ttctagccca
    15901 atttattgaa aatctaaaac actttaaccc tagctctttc aaaagcttta aatcttcttt
    15961 gtatgtatga taaaagtcta ttccactacg cttaggataa tatccattat tatctttcat
    16021 agcaaacttt attctttcta tagtcatatc agactcataa ggtttagata tatctctatc
    16081 tttatcatac atatgtacat cagctaaaca ccaacctttg ccaccttcat tccatgcacc
    16141 ttctaattga ttagctgcta cagctcctcc ccataaaaaa cctttaggaa acttacccaa
    16201 tttaatcact ccttatttta tttatttaaa tcatttacta ttatttacct tatattctat
    16261 agactttact attcctacta aagacatagt tatatttaac tcgctgaata tggtcataag
    16321 agtatcttga gcatgtgcaa ataacagcga atgtgctctt ttctctccac cagcttcccc
    16381 ttgtataatc ttggtttgag ctctatgtgc ttctgtcata aacccttttg ctttagctag
    16441 ttttaaatca gcatttctat aatcaaactt ttccaaatca ttaagagcat gtttaatttc
    16501 tgttctagcc tcacctgcac gaagtattat ttgcattgct acttctacta attcttcatc
    16561 tattatatgt tcttcaattg tcatatttat aatttccttt caatttctaa atttaaacct
    16621 cttgactctc taattttatc tcttctttct cataagcttt aaaaaatgga taccatatca
    16681 taaatagcaa tactatattt attgctaaaa caataagacc tctaacatct tgatttacca
    16741 taaaagtaga tataccaaaa ggaacatacc ataaattaaa caccttactt ggaattgtaa
    16801 caaatcccat actcattgat aaataagtag ttattgatat tactactgtt cctatccaca
    16861 taggtaacat taaaaatggg ttccatgcca ctggagctcc aaatattact ggttcattta
    16921 tattcattat agaagggaca atacatgctc ttcctattgc ttttattctt ccaactctgg
    16981 ataaacacat cattataact aatggtaatg tagcacccaa ccctcctaaa gttatccagc
    17041 ctgtttgaat aacttcgtaa acatatatat tagttgctac tcctcctgat gctactgctg
    17101 ctgcattagc tgcaattgca ccaagttgaa ttgaataagt tataggagat agtacccacc
    17161 cacttattcc taaagaatat ataaatacta ctaagaagtt taagagtaca aatccaggta
    17221 atgtctgtgc tatatctgat actggtttaa atactctaac tattatttca aacatgtcaa
    17281 agttgagtcc ataaattaat ccccagccta caaacattat cactgttata ggtaacatat
    17341 tatcaaacca cttgcttaca aaatccggaa gtgctgaatc ttctccgaaa aatgaaaaag
    17401 aaccaaataa agacattatt aatccagaga ataatcctgc aactagcgat acaagcattc
    17461 cttctcctcc aaacctgttg aagttaaata tagctccttc attcgtaatc tctggacgaa
    17521 tcatcattaa cattaatcca gcacctgcaa gaccagctac tacactcata ctttcaaatt
    17581 tcttattctt cattgtttcc ataggcaata aaaatgctat aaataatcct aaaagtccaa
    17641 atgtatattg atttattgga cttaaatcag gtaaaaagct tatgtaattc ttcaagacag
    17701 aaactatagt tactaatgag cctacaaata ccattggcaa aactttcatt atagttgatt
    17761 gaactgattg tacccatata ttcttagtca tattatttac aacaggagta aatttttcac
    17821 ttaaaaaatc tataaattta ttcataaaca ctctccccat aaatatttta tttgtttgat
    17881 attagttcta gtgcttcttc caaagccttt gcaccatcta gtcttccata tgcagtttta
    17941 gatatacatg ctactggaac gttatattca ttagcctttt gttgaatttc ttccttcata
    18001 taatctaaat gtggtcctag taataaacaa tctatttctg ataaatactt atctatctct
    18061 gactcacttc tcgcttttat atctgcttct ataccttttt tctttgcact ctttctcata
    18121 ctagttgcca taaaacctgt acttgctcct gaaccacacg ccaaaagcac tcttatattt
    18181 ttcataataa tacctccaat atttttatcc attaatttat ttcttaatga actttttatt
    18241 ttattatgtt tatattttac ttcccaaata agttcatttc acataaatat atttcctaat
    18301 aataggaaat catttactct taattagacc tattaatata gtataatgta tcttataata
    18361 cgtacattca attatataat tatcatatca tacagatttt attttaaaac tatgcgaggg
    18421 aatctaatga aaacatctaa aaaagagcta tttttaaatt tcataaaaac gcaacatgat
    18481 tggatagatt catccacatt agctaattac cttaatgtat caacacgttc gattagaaaa
    18541 tatgtaaacg agattaattc taatggtgca tttatattat cttctaaaaa gggctataaa
    18601 gtaaatctca atactaattg tcaaactaag attgatagtt cagagaatat atcacctgat
    18661 aacagattaa atttaatttt aaaagaactt atagttaata gtaatggaat taatatattt
    18721 gatttaagtg aagaattatt tgttagccca gctactattg aaggggatat agtcaaagct
    18781 aataaattta taggaagtta taatttaaag ataaagcaat cgaaattttt attgaaacta
    18841 gttggaaatg agtctgcaaa gcgcaagtta atgagctcta taatatttaa agaaacggga
    18901 agtgattttt taagcttatt tgatgtacaa aaaatatatc aagagtataa tttgactaaa
    18961 ctaaaagaaa atataatata tatattaaaa aaatacaatt tatttataaa tgagtatgct
    19021 ataaacaaca tacttcttca tttaatgata actatagaca gaataaaaaa gaataattac
    19081 atagactctg ttgaagtagt aaactacata gacaataacg tagatataaa tattgcaaaa
    19141 gacatatcaa attttttaga atctgaatac aatataactt taactagtgc ggaattatac
    19201 tatttagtat ttcagttaac aaacaaaact acagtcttaa actacaatca aatggatact
    19261 aaatcattgt caaattatat agatgaacac tttgttaaat taactaaaaa aataattaaa
    19321 aatgtatatg acttatatct tatagattta tctgatgagg aatttgttgt aaaatttact
    19381 cttcatgtaa aaaatttaat cagtagagcc aaaaataatc aagttttaag aaatcaaata
    19441 cctcaaaagt taaaagattc ctaccctttg atatatgata tatctgttta tatttgcaat
    19501 cagattcaaa ctttagaaaa tgtagatata gacgaagatg aaatatctta tatatctctt
    19561 catgtaggtt cattctttga tagacaaaaa ttattagaag ataaagttct atgtgcatta
    19621 attactccaa attactatga tttacaattc aaaatagtaa gagaccttga gaagagattt
    19681 aatgaatcca tagaaattat tcagattttt tcagatactc ataatcttga ttttgacaat
    19741 aaggtagaca tggttattac tactttacca attaacgata gatgccctat accttttgtt
    19801 tatgtaaatc cttacctaaa tagaaaggat tatgataata ttcaaagtaa atttattcag
    19861 attaaagata gaaaaaatat acttactgta caaaatcatt tagaaatgta ctttagtgaa
    19921 tctttgttta tgaaaaatat atatttagat agtgctaaag attatataag atttatggga
    19981 aatattctgt acgaaaataa atatgtcaaa cccaattata tagatgatgt tcttataaga
    20041 gaaaaaatgt cttcaactgc atttaataac aatgtagcaa ttcctcatag catgaaaatg
    20101 gatgctttaa aaaccggagt atgtttaatt gttaacgata aaccagttaa atggggtgag
    20161 gaaaaagttc aaattatagc tatgatacct ataaatgaaa aagaaaaaga aaaattcaat
    20221 tacatatttg aaagctttat tgaaatatta tctgaatgga ataatgttaa agaacttact
    20281 aaggcagata actatagtag ttttatgaat agaatagctt atcttattca aaatatctaa
    20341 atccattaat ttatttccaa tatatgtttc ttaagttatt aacataaaat agataagctg
    20401 tcacactaaa aagtctaaat taatatgtga cagcttttta cctaatatac cttgttttaa
    20461 tactacatat tttttgactg ttgtatacta gttataagat tgaatatttg tgttgttgta
    20521 gctgtcatat tctcactagc agttgcttta tccattgctt cttctattgt catagctcta
    20581 tttactatag aaaaataagc atctataccc tcttcattac atttaactgc atcttctgtt
    20641 gtggctccag ccaaacctat tacttttacc ccatattttt ttgccagttt tgcaactcct
    20701 attggagcct tgcccatagc agtttgattg tccaatctac cttcaccagt tatgacaaaa
    20761 tctgcatctt ttaactcttc ctctaattta atttcttcta atattatttt tattccagat
    20821 tctaattttg aatctaaaaa tgctaaaaaa gcaaaaccta atcctcctgc tgctcctgct
    20881 ccttctacat tagctacatc tttacttaag taatttttaa ctactttgga aaaatttcta
    20941 agcccattat ctaacttctc aattatttct gatgttgcac ctttttgaga cccgtatatg
    21001 tatgcagctc catttttccc aaacagcgga ttatttacat cacaggctat tttaaactta
    21061 cattcatcta attcctttaa tctattttct atttttatat gtcttatttc atttaaaact
    21121 tttccaccta atcctactaa cttgttattt ttatcataaa actcaaaccc taaggcttga
    21181 agcataccta tcccagcatc attagtagca cttccaccta tcccaactat aaaatttcta
    21241 tagcctttat ttattgcttc ctttattatt tccccaacac cataagttgt agtgtataaa
    21301 ggatttctaa gttcagtcgg aaccaatgtt aatcctgatg cttgagctat ttctattatt
    21361 gctgtacttg tattttttaa taatccatat gttgcatcca cttttttagc tattggtcca
    21421 gtaacttcta tagtctcttt tttaccattc atcccttgaa ctaaagcatc tactgtccct
    21481 tcaccaccat cagcaagtgg caatatcttt acttgcgcat cttctttaac cttcaatatc
    21541 ccctttttta ttgcatttcc tgcctctatt gaagataagc ttcctttcaa agaatctata
    21601 gaaattaata ttttcatact tatctactcc tttttattac tccttatttt aaacatataa
    21661 ttttataagc ttaatttatc ttttatttta tactctacat ctctctttgt atacaactta
    21721 taccattaat attcttttat cttataaaaa gggagtgtct caaaataaat ttcttagttc
    21781 atgagacact cttttgtata aaatcaaata tatgtttatt atttcaacaa tgataaggag
    21841 gattatctct attttaagat aattcctttt tagcactatc taatcatttt tagcactatt
    21901 taaccagtat agttttaact tggttcatta agcactctac atttacagag ttattatcta
    21961 ttgatataga ttctaattct ttttcattta ggttaatcca tttagctgtt ttaatatttt
    22021 tgttaaatac tatgttagct gattttgttt cttctttaga attataaaat ctaactacta
    22081 atgcctcttc tttttcagcc ttttttacaa cacttaacac aaggtcctta ttagtttcct
    22141 taagtaaact atatgaataa tctacttcta tttctgattg attcaatttc atagcattat
    22201 atggcatttt attataagtt tgtatttttg ttaaatactc tttagccata tttgcaacat
    22261 ttgcttctag tgtagatttt tcatgagtaa ctattgctaa gtctattttt atatctccta
    22321 tcatttgaga atctggagta ggcaacttta ttcctgatgg acgacctggg cgtcttgcca
    22381 tttcttcttt acctaggaat cctacacttc taaataatgt tatagctatt gtgtcaaatt
    22441 tttcccctac tatttcaaac tctcttgtac tgtttgtaag aacagctact ccatgctctt
    22501 tgtcagacaa tccaacgaat gttagcattg gatatataga atctggtctt tcactccagt
    22561 cttcctgttg ccaaacttcc atagcatcat catacacact tctctttata tgtccaaact
    22621 gattatcaga cacagaaaac tttgaagcta tatttgttgg tatataagtt ctaaccctac
    22681 aatctcttgc ttggttattt atgttgaata ctacatctat tatagcttta tcatttggaa
    22741 catttataac tatatcaaag tcaataaatg aatctgctct attatttttt ctagcttgta
    22801 tatcttttgg tacttttaat ctataattta tctttatctc attattaaat ctatttttct
    22861 ttatagtgga atttgctact atattatcgt taaatattaa tttctcattt ggaagtggtg
    22921 aaaaatcata ctcatctcct tcatctcctc cattttccat caataataca ttttcaaaag
    22981 ttttatttaa ttttttatca aatatcttta atgtcccgtt ttcattaact tgaatagtat
    23041 aaaagtcatt atctaccatg tcacatactt cataagcttt ttctaacata tgttcaactt
    23101 cttttatcat atacgcctta taccccattg caggtatttt atctttaaag tttattgtat
    23161 ataccataaa tgaatcatag tttccataat gaacaatttg acggtctatt aatcctgcat
    23221 ctattacttc tttatgtacc acttcaaaat ccaacttatt attatcttta tcaaatattt
    23281 caaatgcttt cattttagtt ataacttggg cgctcactat ttcattccta tcgtaaggca
    23341 ttaagttaaa tgctattaac ttatctaaag ccatttcaca actcattgca tcaactattc
    23401 ttcttttgta aaatgttatt aattggtcta ctttttcctc agctaagaaa aatctattca
    23461 ttatttcctg gtgaacttta tctgaacaac aacatccaat gctgtcatga gcatgatttt
    23521 tcattatttc tttccaaatt aactctatta atccatgatg atattcaaat cctagagaat
    23581 atgcaataga agctagtggc tccaatatgt tagttatctt tgattctatt ctagcatttg
    23641 ctgattttat atccattctt gttgagaata tacttctatg aaccctcata tatttcccat
    23701 ctaaaaattc cccttttatt gtatctatat cctcattttt ttctagctct ttaaatatgt
    23761 tttcatatct acttaagaaa aattttctgt ctggataaca ttcttttaat ttatatataa
    23821 cttcaaatat attcttttga attggcattt ggtcatgtcc atttggaagt attatatggt
    23881 cagtagttgc acccttatca agaaccggta aatatttatc cattcttgtt cttagctcat
    23941 cttcattcat tggtagatat tttcctattg cataacctaa tggaagtatt tgaaccaaaa
    24001 cagaagaccc atcatcactc ttccaattaa actctgtttt attagttcct tttctttcag
    24061 aacatcctct ccaaaacatt gaatatttta tatcaaaccc atttagtatt tgtggcattt
    24121 gagcactttg accaaatgaa tctggtaaat atcctatttt catatattct ccaaattcat
    24181 cacaatcttt tattccatat agtaaatttc taagtatgga ttctccacca actaccattt
    24241 catcagtttg agtataccaa ggacctatta ttagccttcc ttcttgaact aattttttta
    24301 ttctctcctt acattcaggc ttaactgcta aataatcttc taatatagca gtttgaccat
    24361 ccatcacata atatggatag ttatgattat tttctaacat atccattatt tcttccatat
    24421 tatttacaag taatattcta gattcctcag ttgagaagta ccattctcta tcccaatgca
    24481 tatgaggaac tatgtgtaca ttttttttca ttgcttatcc tccaaaactt atctgataat
    24541 attttctgtc taattaaaaa tccaataaaa atactactga gtttacaaaa ttttttaata
    24601 tttattttat cactataatt aaacctttga tttaaatatc taataacgta tcttttaatg
    24661 tatctttctt tgattttttt gtttcctagt tattattaat aatattgctg atattatagc
    24721 tccaagtact gccgcaaaga accatattga tgctataata aatcctgaca tacttccctc
    24781 taaaagcgcc aatgagaata tccccgctcc aggcacttga agacctatat tgaagaatgc
    24841 tactatagac ccagttatgg ctgaaccagc taccaatgat ggtataacac ggattgggtc
    24901 ctctatcata aatggtattg caccttcagt tattccagcc aatcctaata tccaagtttg
    24961 ctttcctatt tccttttctg attctgaaaa taaatctctg cctaaaacag tagctattgt
    25021 aactccaaaa gctgaaacca tcttaactga tgcaaatatt gcataaggta taaaatttcc
    25081 acttgctatt gctcctattg caaatgtata ggctgcttta tttattggtc cacctaagtc
    25141 aaatgaaacc atagctccta ttattgcacc tagtagtatt gcatttactc cagtcatccc
    25201 atttaaccaa tttataagac cattgttaag tgctgctaca ggctttccta ctacaaataa
    25261 catcaataaa cctactacca aagaaccaac tactggatat acccaaaaac ttacgaatcc
    25321 agctaatgtg cctttggctg gtatattctt ctttaaaaat tttactatat aaccagcaag
    25381 taaacctcct aacatacctc caagaaatcc actacctatt aaatcagctg ctagacctgc
    25441 tgcaaatcct ggtccaagac ctggtttttc agctagtgag taagacatat atgctgataa
    25501 tactggaacc atcaatgtac ctaacatacc tccagaaagc tttcttaatt gccataacca
    25561 agaaccttct tgattgtata gttcttgtag accaaatgct tgagatgcta atactgcaaa
    25621 tgcaagtatc atacctcctg ccacaataag tggtattata tgagatactc ctgtaagtat
    25681 tgctgatttt acatcttttt tccatgatga tttttcacaa tatgatatat cagatgattt
    25741 ttctatataa tcacctttac cttgtttttg tgctacttct aaagcttcgt atattatttc
    25801 ttttgctctt tttagtggag ctgctactga cgtttttatg tttggcaaat gttcaaatct
    25861 ttctgtattt ttaggtgcta catctgttgc atatattatt gcatctgccc tttttattat
    25921 atctggcgta tgagggtcca ccataccatt agctccttgc ttttctacat acacatccac
    25981 acctatttct ttaccagctt taattaatgc ttctgctgac atatatgtat gtgctatacc
    26041 ggcagggcat gctgttattg ctaatataat tttttttgat tcattttgaa tcctaatctc
    26101 ttcttttatt tgttctttct tttcttttat atctaagtta ttaaataact ctttcttagt
    26161 ttttgaattt aacaacatat tcatataatc ttcatttgac agtctagtaa ctaactcaga
    26221 aagtatttgt atgtgtgtat ttccaccttc actttctggt actgctatta aaaatattag
    26281 ctctacttta ttatttgggt ctacgctttc ccaattttct attggtgtct ctaaggtcat
    26341 agctgcaaat gcagcttctt taactgactt agattttcca tgaggaattg caagccctct
    26401 ttcaaaacct gttggagata aagtttctct gtacattaca gcttctaaat attcctcata
    26461 agatgttaac ttgccttcat catatagctt tcttgctaag aactttattg cgtcttcctt
    26521 tgtttttatt ttttgtttta aaaaaacaag gttttcattt gtgattttct taaattccaa
    26581 aattacgccc tccttaaatc atttaatgct atgaatatag cataaaataa attatgaaaa
    26641 tgtttttttg aaattataca cacaatttct tcttaaaaaa taaagatttg tgtatttata
    26701 cacaaatctc tatcttaacc aatattgctc tattattgct ctcataatca tcataattgg
    26761 aaccttatct gatatattgt acccatttac ttgtttaatt gttgaatagc aatataaact
    26821 tatatctgca atttgttgca attgattttc acatatatgt gttaaagcta ctaccttaca
    26881 acccttatta actgctgtct ttgctgcttc tataacttct ttcgtttctc cacttaaact
    26941 aagtgcaaaa aacatatctt gttcacctaa ttttcctata tcatgaagta cttcatgtct
    27001 ttgactatag taagttatat tatcatgccc acctctaatt tcttttacta atatctcaca
    27061 taaaggaatg ttttgaccta ttccataaac tataatactc ctagattttt gaattatatc
    27121 tgctacttta tttatttttt cataatctat tagctctaat gtctttttta tatctgattc
    27181 aaaaccattt tttggctctt tatcctcatt ttttaactct tcttttaaat tattttttag
    27241 ttgagagaaa ccatcatact ccattttttt acatagccta actatggtat ttgggacagt
    27301 aaaaaattta tctgctaaag attgtattga taattttact atttcttctt tgttttttct
    27361 catatattca attatcatgt cttctgtatc tgtaaattta tactcatatt tttttgctct
    27421 ttcataaaat tccatagttt ctcctccatc tattttatta ttcataagta aaattcaaaa
    27481 atattagcta aaattttcgt taatataatt ataatttaat atttttaatt tattaatgca
    27541 tactactctt tttattatac ataaaaaatg tgtacttcct atattgatag agatgattca
    27601 agtttttatt ttgatacatc tctatcaata tttttttaga aaatacacat ttttttcaaa
    27661 tacttatttt taattaaaac tttaggtaat ctattcttta tatataatta ttagacttga
    27721 aaatatcttt aaaatcaacc aattcaatat tatttctttt taaccacatt ttaacttcct
    27781 tatcacatag cattgcatgt tcatatactc tctcttgaat aagagaagat gattttataa
    27841 gactataatc tataaatcct ggatgagtaa ccatcaatgt tatatcttta tctaaattta
    27901 tatatttttc ttcaaataat ttaacaaaag atttatttaa cttgtaaaaa tcattctgag
    27961 gatatttact tggcatgcta atacatgaat ccaaataata gggacaactt ggtataccgt
    28021 attcttttgc agcatcacac attgctgata ttgatacatc ttcttctata ccatgtatat
    28081 caatatactc aggcaattta ctcatataca acttgtaagt ttcaatttga gcacaaactt
    28141 cttcatatac ttcttcataa ttaaaaacct ctttacctgt cagtagttgc gttcttctta
    28201 ttttgctaga aacaaatttt ccaccttcat ctaacaaatt ctttatctta atatgttctt
    28261 tgcaacaagg ttctcctatt actaaattaa catgtaaacc aaaacataca tcatcatttt
    28321 ttaccatttc tatagcttgt cttgaaaaag gcatattcac catgattcca gcacttgtaa
    28381 caataccgtt cttataagaa gatattatcc ctagtgtaac accttctgaa tatcctatat
    28441 catctgccct aactactaat ttcttcattt tatgcatctg cctgctttct caactcatca
    28501 agttgcattt ccatctcatc ctttacttta acaacatccg ttcctatgat gatttgaact
    28561 gatgtattac tcaatttaat tacccctttg gctcctgttt ttttaatatt atcatcattc
    28621 aaaatagtgg catccttcac ttctaaacgt aatctcgtca tacaattatt aagagtcact
    28681 atattttcat aaccaccaca atttttaatt atctgtttag ccatatatga aaaattacca
    28741 tgagaaagtt ttaaattctt ttctttttca ctaactttat cacctatctc tatctcaact
    28801 tcacgtcctg gtgtttgcac attatctttt attattatat atttaaatgt tacataatat
    28861 aatacaaaga atacaactcc tacaggtata actaaccacg ctttatctcc ataactaaaa
    28921 cttaagacat agtcaattat acttgctcca ttaaatgaac ccaagcgagc acccaataaa
    28981 tatagtacaa ctccacctag accagaataa aaagaatgaa ttatatataa ctttggagat
    29041 gcaaataaaa acgaaaattc tattggttca gttactgttg ccataaaaga agtcaatgct
    29101 ccagcggtca ttgcaccttt tacttcttgc ttctttttat cttgtgctgt tttatatata
    29161 gcataagaaa tagcaggtat tgaaaacatc ataattatgt aaaatcctgt ccagaagaat
    29221 cctgcatttg ggtctcctgc cataaatcta ggaatttctc ctcttataac ttctcctgtt
    29281 gatgttgtat attcacccat ttcaaataat atgtaagcat ttaaaacttg atgtaaccca
    29341 gttggtatta gtgcacggtt acagaataaa aacaagaata ttcctattgc accagctcct
    29401 cccaaccaaa aaccaaattg attcattgca gcttgtggat atatccataa taaactaaat
    29461 atatatgata aaattaaagc aaccactggt gatagtgtaa ttacacattt ttctcctgca
    29521 aagaagctta aaaatgctgg aactttaact ttatatgttt tattgtaagt ccatgctata
    29581 caagctccaa caattatccc accaaataca cctgcattta atgtatcatt caataactct
    29641 aaatttgact taaatatcat atatgcaact acggctgcaa ccattacact tgtcttgtct
    29701 ttagactttg agtaggcact agctgctcct aataaaaata agtagtccac atttgaaaat
    29761 actatattag atatattaga tatagctggc acattgagca tatttggacc aaataataat
    29821 cctaatatac ctgctgctgg cattacagct attggcacca gcatagtctt tcctaaaagt
    29881 gacagttgtt gcatcatttt gtttaagaat ctcttcatat ccaatcccct ttctatttcg
    29941 gtttaatttt ttgtgaaaac gatttattca cattttatat tttaaatatt atatttgttt
    30001 aaacaaattg tcaatatttt ttattcattt tatttttatc atttagttta acatataaat
    30061 ccttttaagt gtattgctat atctagtcta tttttttaat tcattttttt tattttaaaa
    30121 atatgtttaa ccatgtattt gaaacaaccc acaaaataat tctattttgt cctttttatg
    30181 gttgatatga ttctattttc actatacatg tctaaacaca tttgatataa tttgttcata
    30241 tcagttcaat aatattacta aagaccttta aggagggata ggggtatatg gctaaaaata
    30301 aattttcaga gatatatgaa gtattgaaag aggaaatact tgatggtaaa tatacaagta
    30361 atatgatgct gcctactgaa cttcaactta ttgaaaggtt ttcatgtagc agaaacactg
    30421 tacgtagagc aatttctcaa ttaaacacag aaggatatgt tcaaagtata aaaggcaagg
    30481 gtgttgtagt gttagaaaat agttgttcta atgatttttt tctaaatatg cacaatttca
    30541 aaggtgtaga atcgattgta gaagataaaa aggttaatac tgctactagt gttctccact
    30601 tcagcaaaat cttaattgat aataagttat ctaaaaagac aggttttaaa gttggtagtg
    30661 aagtatatta ccttcacaga ctaagatata ttgataacat acctaaaatt ctcgacatta
    30721 actattttct atgtagtata gttaaagacc ttgatgtttc tatagcacaa ggctctattt
    30781 ataaatatat agaagagtgt ctaggtacaa aaattgtttc atcaagaaag atatttaaaa
    30841 ttgaaaaagc tacagaactt gagttaaaaa cgctacctct aaatgattat aattgtgttg
    30901 gtgttattaa aaatagtgta tatactgatg atggaaagct ttttgaatat actgaatcaa
    30961 agcatacacc tgaaacattt gtattcatgg atgttactca aagatattaa aaaaattatg
    31021 tttaattaaa cataattttt ttacgtttag acactttatc ccatactaac ctcactattc
    31081 tatatacaat tatatgttta tacaaataat tatttattgt atattttagc tttaatatta
    31141 gtttaacgct tgacaatttg tttaaacata ttataattaa cttataaatt acaggaggta
    31201 tcaagtatga aaaattggtg gaaaaaagca actgtctatc aaatatatcc aaaaagtttc
    31261 aaagatagca acaatgatgg tataggtgat ataaatggta ttatagaaaa attagattat
    31321 ctgtattctt taggtgttga tttattatgg ttgactccta tgtacgtttc tcctcaaaga
    31381 gataatggat atgatataga agattattat aatatagacc caaaatatgg gacaatgagt
    31441 gactttgaaa aactcttaaa agaagctcat aaaagagaca taaaaataat gatggatatg
    31501 gtcctaaacc atacatctac tgaacataaa tggtttaaag aatctaaaaa aagcaaagac
    31561 aatccatatc gtgattatta tttctggaaa gatgctaaac ctgatggttc tgttcctaat
    31621 aactggatat ccagatttag tggaactgct tggaaatatg atgaaaccac taaccaatat
    31681 tatttacatt tatttgaaga aactcaagct gatttaaatt gggaaaatga aaaggttcgt
    31741 gaagaatgtt acaaaatact agaattttgg gctgataaag gtattgatgg ttttcgttta
    31801 gatgtagtca acctactctc caaaacacct ggattacctg atgaccctat aacaggacca
    31861 aaaggagatg gaagaactca ctacgctgat ggtcctcgta tacacgaata tttacataat
    31921 atgaatcaaa aagttttcaa acctaaaaat atagttactg taggtgaaat gtcatctact
    31981 acacctgaag agtgtatcaa ctatactaga gaaaatcgag aagaactatc tatggttttt
    32041 agcttccatc atatgaaaac agattataaa ggaggcgcaa aatggactaa tgaaatgttc
    32101 tctctggata aattaaaaaa agcacaatct gactttcaat ataaaatgta tgaaggaaaa
    32161 ggatggaatg ctctatttta ctctaatcac gaccaaccta gagctttatc tagatttgga
    32221 gatgatagat tttttcgtca agaaagtgct aagatgttag ccataacatt atttggtcta
    32281 cagggtacta catacatata tcaaggtgaa gaaataggta tgacaaatgc ctactttact
    32341 aaaatagatg aatatgatga tgctgaaagt aagaatgcat actatcatat gattaaatct
    32401 ggtattgatg aaaaggaggc attgcttata cttcaacaaa aatccagaga taatgcccgt
    32461 actcctatgc aatgggataa tactatttat aaggggtttt ctaatcataa accgtggtta
    32521 aaagttaata atgacaatat tagtgtagaa aatgagctta atgattctcg ttcagtattt
    32581 tatacttatc aaagattaat caaatatcgt aaacaatatg atatttttac agaaggtact
    32641 tatagattgt tagatgaaaa tcataaaaat ctatttgttt atgaaagaga gtataaaaac
    32701 caaaatcttc ttgttataag taattttact catgataatg tagtatataa attacctgaa
    32761 acttatttga ataaattaaa ctacacaata cttgaaacca attacactag agatataatt
    32821 gaaaataaaa tggaaataaa accttacgaa agtataatga tatattataa ataaagttat
    32881 ttatgtaaaa atcggtataa caccctggtg ttataccaat tttactaaat tcaaagtaat
    32941 atttatagaa tatctataga ttacaataat tatttgactt ctacatcatt atatttcttt
    33001 gaatatactt tagtcatata gaaatatata ggaactacaa ttatcatccc acctaatccc
    33061 caaactatct tcataggttc atcaatacct gcttttatca gtaataatat acttataata
    33121 actgcaaata ttggtataat tgctcctcca ggtattctaa aatttacatc tttaccagca
    33181 tacttctttc tcaatattag tacagctaaa catgtaggaa tatattgaat aaatctagat
    33241 acaacactta tttgagataa tgttgtgaat gaacccgacc atgctattag taaagttcct
    33301 atgacagata ctataattgc tatataagga gctccatttt tattagtttt tcttatagac
    33361 ttaggcatta atccttgttc aaccaaagca gcagcagacc ttggtgtaaa tatggatgat
    33421 gcaatattta tacctccaac agatactata gttcctattg taattatata cccacctata
    33481 ggacctaaca tcttaatggc agcatctgca acaggagtag ctgaccctgc taagccatta
    33541 cccaacaccc ctatacttat tcccagtata agcatatata ttactgatac tacaaacatt
    33601 accattacca atgctttagg tacatttttc ttagggttct ccatatcttt tgctgcaaca
    33661 gctaaagatt caaatcctgt aaaagcataa aataaagtta tagatactgc tacaaaagct
    33721 gatgacgcac tttgaccttt ttgtattatt acaaatgggg taaaattaga accctttata
    33781 aagaataatc ctacagctat aaataaaact attggtaata attttcctat agtcatgata
    33841 ttatttaagt gcttagattg tttcatcccc ataaaattta taatcccaag tcctacaact
    33901 aatataccaa ttattatatt tttagtaagt ggttctgctg cttgtggcca tacagatgat
    33961 agtgttacag caaagaaatt tgccatagta gcccaagcaa tcatactaac tatccacttc
    34021 ataaatccaa cttcaaatcc aacaaaatct ccaaatgcct cttttgcata aagatagggc
    34081 cctccatcag tatcaaacca actacctgct tcagcaaaac ataaagcaat agaaacagct
    34141 aaaagcatac aaaaaactaa tactaatata cttgctggac ctatatctgt ataagcatca
    34201 ccaggtaacc cgaatatacc tgaccctatt attgcattga ttcctaatag tacaatactc
    34261 caaaaaccta acttaatgcc tttttccatt gttattcccc ccaaagccaa cttaatatat
    34321 ttgtattttt tatttagatg cttctttaac tattgccata aatatttttt tcatattatc
    34381 ataatcccta gtcatcattt ctggatgcca ttgtatccct attacaaact cacttccttc
    34441 cttttctata gcttcaatta aaccgtcttt agatgtagct gaaacaatat atcccggtgc
    34501 tactttgttg acaactaagt gatgaaaact attaactaaa acttcttttt ctcctaatat
    34561 ttcatataat tttgtcccct cctttatctg tactgtatgt gtaggtacat tagatagatg
    34621 ttgctgattg tgtttaatat aagcaccttc aattaaagat aaatcttgat acaaactacc
    34681 tccttcagtt acatttataa tttgttctcc tctacatata cctaaaactg gttttttcat
    34741 atctaaagca tgttttaata atttcatatc aaaactatct ctctttggtg atattgcccc
    34801 caacttatta tgaggttctt ctttccatat gagtggattt acatcttgtc ccccagatag
    34861 tataagcgca tctatattag atacctgctc ctttataatc tcatcatcat atacaatcgg
    34921 tacaatatat ggtatagctt tacacataac tactgattgt atataatcat tatttacata
    34981 agccctttca tagccaggaa acattcctcc ttcatcaatt agtaaattac ctgatattcc
    35041 tattattgga tatttctttt tagccaattt cttcacccct taacttattt gtgaacaata
    35101 aaaaatgcta cttctcttaa agagcaagta gcataccaag attttattta tttgaacatt
    35161 ataatttatt ttagaaatac tttttttgaa tacgagtaat ataaataact ttatttactt
    35221 atattttatt ttaatcctgt ttagtttgac ttttttaaat tgttttaagt acaactaata
    35281 ggtaaaatag gcatttagat tttccttttt atcaacctat taaatactgt ttaatatatt
    35341 ttttcccctt ctatttaatt cacttccctt tctaccagat tcacatatta tcaaatccag
    35401 ctcttttaac atattaagta ttccccttac ctcaccttct gttatattgt agcctaattc
    35461 atttattgaa ttaacaatac catttctacc tatattttta ttaagattat tggcattttt
    35521 taaacactcc attactaact ttactttctc tatagatgtt ctatccttta atatacttat
    35581 ttctttacta aaatcttcag ttatatttaa taagttatga ggtaaatcat ttattaaaat
    35641 aacatcctca gaactcataa tgttaatata catagcagta ttttttaatt ctcttatatt
    35701 acctggccat tcatatgcat taataatgtc ttgaacatct tgactaattg ctctcttcct
    35761 tttcataaaa tgctttaaca taacttctat atcttctttt cgctctctta aaggtggtat
    35821 atttatgggc aatacattta tcctatagta taaatctgct ctaaatctat ctccttttat
    35881 catttctaat aaatttttat ttgtagccgc aattactcta acatctatat ctattacatt
    35941 ttctcctcca accctcatta cttgattctc ttgtaatact ctaagtaatt tgctttgtaa
    36001 gtatataggc atgtctccta tttcatccaa aaatatagtt ccattgttag cctgttcaaa
    36061 taatcctttt ttacctcctt ttaaagctcc tgtaaaagca ccttcttcat atccaaaaag
    36121 ttcactttca agtaaatttt ctggcatagc tgcacagttt atcgctataa atggctgact
    36181 atgtcttggt gatgcattat gaatagactg agccatgagt tcttttcccg ttccactttc
    36241 tcccgttata agaattgtat aatcagattc agctattttc atacctaatt tcttagttct
    36301 ttccatcttc gttgaaactg tttttatatc atcaaaagta tatctagcaa cttgtccctt
    36361 ttctctaagc ttctttgata ggttttgctc caattttctt atgtaagtta tttcttgaat
    36421 acaaaaataa tatccagtaa cccttccaaa actctctata ttatacttat ttacgtttaa
    36481 gtacttatta ttaaatttta taacttcatc atgtgcctta ctcaaactaa aaatcttttt
    36541 aatattctca ttctctaatt catctatatt tttccctata atattacttt gaatatctag
    36601 tatatcccct aaactattat tgcaaataat tatgtttccc ttatctgata ccattgccat
    36661 acctaagttt gataaattta ttatagaatt taattcatct gcttttatag taagttcttt
    36721 atatttagta tttattcctg aatataaact aattatttca tctgtatact taattagcct
    36781 ttcagtaatt tctttatcat ccagtttgag ttttgctatt atttgaataa acgttgaact
    36841 atcaatatac ctatggccta aatcaataac ttcttctata tattttggaa ctaaacttgc
    36901 ctctcctggc gttatagcta tcttaatatt tctatattct tcattgggat tgtatggtat
    36961 aaaattcaga tgatttattc ctatattgta taaattagat attgtttcca atacagtatg
    37021 cttattatcg tttactacta atacatctat tccttctggc aatgaaaata acttatatat
    37081 atccttttgc tttatgctcc tatttatagt tattatcttt gagacatctt ctacatattt
    37141 ttttattttt aatactctat catctatcat taccaatatt atgtcatctt ttattaattc
    37201 cttctctgtt attttattta aataataatt ttttatcatt actttatctt taaatatcat
    37261 ttctagattt ttatttaaga aacattctaa tttagacaac ccatcagtta ctactgctat
    37321 tgttttcatt tgattactcc ttttaaaata tactctactc ttttataata tacatctgat
    37381 tttcacttta acagcacaaa aaattgtaca tttagtatac taacatattc ctttattagt
    37441 tttatgtccc taaatatcat agttgcaata tctatcatat ttcttaaact tctataaagc
    37501 aaaaattgtg ccaaattaat aattttctta attttattct aaagttcctc aatagtattt
    37561 acagacttag cacttatagc ttataagtct ttatattatt aatctatttt tataggttaa
    37621 ttgtacaaat catatatcac ttttacccat taaatgcatt ataaaaaaga tggaataacg
    37681 attcgtcact ccaccttttt tgtaataaaa aattgtatat ataaacacta tttactactc
    37741 ttctaagtac aaacattatc tacatactat aacctttcac cattagattc tattacttgt
    37801 ttataccagt caaaactctt tttcttaatc ctagacaagt ctccacttcc atcatcatac
    37861 ttatccacgt atataaaccc atatctcttg gccatctcac cagtagatgc acttactaaa
    37921 tctatacatc cccacggagt atatccaaat aaatcaacac catcttctac agcttccttc
    37981 atttgctcta tatgtgcttt taaatactct atacgatagt catcatttat cactccatta
    38041 gattcttttt tatcataagc tcctaaacca ttttcaacta ccatcatagg tattctatat
    38101 ctatcataaa tttcatttag tgcatatcta agcccttcag ggtctatttg ccatccccat
    38161 tcactagact taagatatgg atttttcact ccacctaata tgttgccact tgtattatct
    38221 tctttagaat cactacttac acatgtagac atataataac taaatgtata aaaatctaca
    38281 caaccttctt ttaaaatttc taaatctcct ttttccattt ttatttcaat attattttct
    38341 ccaaaatatc tattcatata agttggatat tcgccacgca cttgtacatc tccacagaaa
    38401 taattcatta ctctactatc tctttgagtt ttaactgcat catctggatt acaagtataa
    38461 ggatacattg ttgcaaatat ttgcatacat ccaacttgat aatttgaatc tatttcatga
    38521 gctaacttaa cagccttagc acttgctaca aattgatgat gtaacccttg aaaacgaagt
    38581 tgaggtatat ctacttggtt tataaaatct gtagtccctt catttaatat tccttgtgat
    38641 aagtagcctc ccataggcat tgtagctgaa tttatttcat taaatgtcag ccaatatttt
    38701 acttttcctt tatatctttt aaatattgtt tcacagtaat tcatatacaa gtctattaca
    38761 tctcttgaaa cccatccatt gtatttctct gttattgcaa atggtatttc atagtgagat
    38821 attgttatta atggttctat attatatttt aaacattcat ctatcacatt ttcataaaat
    38881 ttaagtcctg cttcatttgg ttctttctcc attccagttg gaaatattct agtccatgct
    38941 attgaaaatc taaatacctt aaatcccatt tctgcaaata aagctatatc ttctttgtat
    39001 ctgtgataaa aatctattgc ttcatgactt ggataaaatg ttcctttttc tataactctc
    39061 gttattcttt tacttacttt ttgagaacct ccagtacaca tatctgaaca agaaggacct
    39121 ttcccatcta aattccatgc tccttcacat tgatttgctg ctgttgctcc accccataaa
    39181 aaatcttttc taagtgccat aattctacct ccaataattt atttatagta actttatttt
    39241 aaaataaaaa tacatatata taattcttaa ttaatagaac tctcttttac gctaaaacag
    39301 ttatcaattc ctgactatta tttacatgct tttgtgtatt ttctaaaata tccacataat
    39361 cagaataatt agtaacaact actggtgttt ctaagcaata tccttctttt gaaatacttt
    39421 ctatgtcaaa agttattaat atctgacctt ttttaatctt atccccttga ttaacaaaag
    39481 atttgaaata tttaccttcc aactgaatag tatttaaccc tatatgtatc aatagttcac
    39541 aaccattatc agatataatt cctattgcat gttttgttgg aaataaagtc attacagtcc
    39601 catcaaatgg tgccactact tttccttctt taggtgttat tgctacacct tttcccaata
    39661 tacctgttga aaacgctgca tcttgtatct gctccaaagc ctttacttca ccttttattg
    39721 ggctatatac tacttctctt ttgttttttt ctataacgcc ttgcccatta tcatctttag
    39781 aaatttctac tatagcatca tctttccaaa aaaataatgt taaaacaaat cctactacaa
    39841 tagctactat tattgctatt atagttgcat aaacaccttt catatctcca gttgttggat
    39901 ttatataatt aggtatacca aatattccaa gtccacccat tacataactt tttacattca
    39961 ttatccccat tacagctcct gccacaccac cacctacaca gctatatatg aatggtgttt
    40021 ttcttggtaa agttattcca tatattgcag gctctgttac accacatatc cctgaaataa
    40081 ctgcaggtat acacagctct tttatcttct tatcttttaa tttaaagtac attgctgcta
    40141 ctactgctgt ttgtgcaaaa gatggtacaa atgctcccac taatattgtg tcaaacccca
    40201 tagtcataag gtttgacata gctattggta ctaatcccca atgtagacca aatattacaa
    40261 gcacttgcca caataaacca gttaatgcac cacataaaat tggtgaaaag ttaaataaag
    40321 ctaaaaatcc atttccaact atattagaag cataggtagc tataggccct ataaccataa
    40381 atcccaatgg taaagaaact aataaaactg ccattggtac aaagaaattc tttactacat
    40441 ctggtattat tttagcaaac actttctcca acttagctgc taataatact attaaaatta
    40501 ctggtactac tgttgaagtg tagttcattg ctattactgg taatcctaag aaagttatat
    40561 atactggtga ttcaaatatt gttccagtaa atagattgta taaaggttga cctgtagatg
    40621 ctagagtatc taattgaata cctgaatagc ataaaactgc acctataagc attccaataa
    40681 atggttttaa tttgaatttc ttagcagctg tatatccaag aattattggc atatactgga
    40741 acattgcatc tccaattgca tttaacataa tatatgcacc atcagtttct ccatacaaac
    40801 ccaatgctgt aaatactgca ttaaatcctt ttatcatacc agcagcacac agaactccta
    40861 atattggttg aaaaactcct gatattatgt ctataaactt attaagtaat ccttttggcg
    40921 catctgagtc tgaattatct attgtatctc ctcctaatcc agccaaatcg cacacaactt
    40981 cataaacatc tggaacatga ttaccaataa ctacttggta ttgacctgca cttttcatta
    41041 cagtaactat accatccatt ttttttagca catcatcatt tgctattgac tcatctttta
    41101 atttaaaacg tagcctcgtt atacagtgag ataaactgct aatattatcc tttcctccta
    41161 cattcgcaat gatttctttt gatagttgtt catactttct cattttttct cctccatttt
    41221 ctttttgaaa tatgaaggtt tggccaactt aatgtcacca cccaatatat ataaacctaa
    41281 ttattaggat ttatatctga cttaatactt ttacttacca ctctttcaac gtgaatagtt
    41341 aagtataatt tttcctcatc tgttaaatcg tagttgtatt ttttatgtat aaattcatcc
    41401 aacttttcta tgaatttata agcattttta tatttaactt ttattacatt taataaatca
    41461 tcttcattat tttcatgtaa attattacta acaattcgtt ctgcaaaata ctttaagtga
    41521 gtaataaatc tatgataata aactgaatct tcatcaaata ctattctgta atggtattta
    41581 accatatttg aaatttcttg tattatttgt gtaatctcat atatagtttt tatactttga
    41641 tccatttgtg cattaactat atgcagtgct ataaatccag cttcatcttc tgatagtcta
    41701 atttcaaatt ttttttctat ataatccaat gcctctaacc ctattttaaa ttcactctta
    41761 taaaatcttt ttatatccca aagtaataca ttttttacac ttactccttc acgagctctt
    41821 tctattgcag tatacatatg gtctgttaaa gaaatataaa tactatcatt taatcttttc
    41881 cctagttttt ctttagcata taaaattatt tcatctgata attccatata tcttatagga
    41941 atatctgcta ttagttcttt aaatttatca gatatatttg ggtctgaaag tttgaatatc
    42001 ttatcaattt tatcctttga tatatgctct cctgtacgtt ttttataagc taaacctctc
    42061 cccataacta ctatttcctt atgattttca tcagttgtaa taattacatt attattgagt
    42121 attttttcaa taatcattgt gatgccccct ttattagcat taagttaaaa taaatctttt
    42181 ttaattattt agaatattta aaatgtaatt tattacataa aaaaaaacct atttattaca
    42241 cattaaatag actcccatga gtcctgagta ataaataggt ttagcttgta tttaaacaat
    42301 cactatccgt ttgtatttta ttttaattat tatttattat atgttagcaa atttattttt
    42361 tagtgtcaac tttttctcaa tattttaatt ttaaataaat tatataaatt gaatcttcaa
    42421 agtactattt ttatcaataa acaaaataaa aaaggtatta ctttctttaa gtaataccca
    42481 tttattttta atttaagtgg tttttcttaa tctcatcaag tagtccatct tctaaataaa
    42541 tttctaatgc acatcctacc attgccttta ctgctttgtg aagtttatca taagcttcct
    42601 cagaatttgc ataatttaga aattcttttt catgaacata taccattgga cttatatcta
    42661 aggctatttc tgtatacata gtagggcaaa cttgacttac attacctata tctgtagaac
    42721 ctactggacc atctttacct tctaatatat ctgttatacc tgcctcaatc aaattattct
    42781 ttgttatctt ttgaagtact ttgttattta ttatgttgtc aaaagaattt tcaaagtatc
    42841 tataagaaag cttagctcct gtcattaact ctgcaccttt tgcacagttt ataacttttt
    42901 tagttacttc atctaaataa cttctttcag cagctcttac gaaaaactta caagctgctt
    42961 tttctggaac tatatttgga gcctctcctc catttgaaac tataccatgt attctaacat
    43021 ctgattttac atgttgtctt agtgcattta tcccagcaaa agtaagttgt actgcatcta
    43081 gtgcattgac tcccatatga gggtgactag ctgcatgtgc tgatacccct tcaaaactaa
    43141 actctaatga atctatcgcc aaagccttac agtttatatt attaaaagct tctatatgca
    43201 tttgataact tacatcaaca tcatcaaaag ctcctgcctt aactaaatca cattttcctc
    43261 ctgttgtttc ttcagctggt gttcctatta gtacaatttt tcctttaaaa ttttctttta
    43321 acttacttaa tactatacaa gctcctagtg tagatgctgc aatccagtta tgtccacaag
    43381 catgagctgg ttctttattt tctccatatc caggaagtgc atcatactca gctaaaaatg
    43441 ctatggtagg tccatcatta tctcctaatt cagctctaaa agcagtctcc atattacaat
    43501 atggataaac tgtatcaaat ccatattcct tcattttttc tactaagtat tttgatgata
    43561 tgtattcttc ttcccctaat tcaggattgt taaatataaa attacaaact tcttcaattt
    43621 cttttccaag ttcattctct atctcataag ctctcttctt tatactctct atcataattt
    43681 acctcctact agaatcctag atataatcct attgtcaaaa atgccattac aagaattgtt
    43741 aatactacca ttaaaggaat tacgaatcta acccactttg accaaggaat attggctact
    43801 gctagtgctc ccattaaaac acctgatgta ggtgttatca agttagttac acctatacca
    43861 aattgataag ctgtaactat catttgtctt gatacatcta ctaagtcgcc aagtggtgca
    43921 agtacaggta ttgtaagtga tgcaagccct gatgctgatg gtattaaaaa tgctattaat
    43981 ccttctatta agaaagttaa atttataaat atagtttttg gtaatccatt taataaattt
    44041 gctgcagaat ttaaaattgt atctattata aatccatttt ctgctattat tacgattcca
    44101 cgtgcaaatg ctatacacaa tcctgctgta attaaatctc ctgctccact tataaatgaa
    44161 ttaactatct catcttgttt aagccctgat gctattcctg atattatacc tatagctgta
    44221 aatatcattg caatttcagt tatgtaccat tctagtctta aaactccata tatcataatc
    44281 gccataccta ttgtgaaaat ggctattacc atggcttctc tttttgtaaa ttcctttata
    44341 tttgtatcac tattaaccat taattcttga tttagtgata tatcatgtac taaagatttt
    44401 ttagggtctt ttttaacttt attggcatac atcataacaa atcctataga tatagccata
    44461 tatattataa acataacaac tctaaactct attccagaac ctggtactat ttgtgataat
    44521 gcttgtgcta ttcctacaga gaaaggattc gtagtagctg ctccaaatcc agctgttgct
    44581 cctataaata ctgtggccac tgctgtcaat gaatcatatc ccatactggt cataagtgga
    44641 ataaatatca tataaaaagg tagtgtctct tcactcattc caaacgtcgt cccacctagt
    44701 ccaaatagaa tcattgaaat cggtattatt aaaaattgaa acttcttcat tttatttaca
    44761 gtatgagcta taacagcttc tatagctcct gttttattaa ctatcccaaa agctcctcca
    44821 actattaaaa caaatccaac tacttcagct gcatctataa acccgtttat aggtgctgta
    44881 aatacatcag taattccttg tggatttgat ggtgcttttt cataagttcc agctacaaca
    44941 actgaccttc cgtctatatc tactctttca aaatttccac ttggcactat ccaagtaaga
    45001 attgccatta acactattag caaaaatatt attgtaagtg tctgtggcat agtaaacttt
    45061 ttctttttta aactgctcat ttttaatctc cccacttttt aaattaaata ttttattatt
    45121 gcatcccttt atcctatttt gtatagatgt aaaaaagctc aacatggata catgttgagc
    45181 taaatgtgct aaatacaaca tacctgcatc ttccctttat cttgtttaag atagctctcc
    45241 atcaaaattc gggataattt tgatgacagt actgtaccta ttaaatacag tcccagcact
    45301 agcttcggac actagcactt cggcacttac tccttttata ttgcctcatt ggtttccaac
    45361 ctcttcaata ttaatcttag taaatgcaac ctctacctca atctgtttgt tagatgaggt
    45421 aattcttatt aatttttatt aattcaaagt aattgtagca taatatgtcg agattgtaaa
    45481 tattttcagt ataataattc ttactttatg atttcattcc atttttcaca aataattttt
    45541 tctccatata atttaaatac tgtagtatat tttaaataca cacaaacttt ccacaaattt
    45601 taaattatta taataccttt ataataaaaa ataaaagcta tgtaaactta tcaatttaca
    45661 cagctttata tacattaatc tactccaagt acagctttaa gctttgatgc tattgtaata
    45721 actcgtggac caaatataac ttgaactgag tttttctttt taacaactcc acttgcaccc
    45781 aattcattta cccagaagcc atcttcatag aatccatcaa ttgaatttag ctctacccta
    45841 agtctagttg cacagtttgt aacatttttt atattattac ttcctcctaa accttctatt
    45901 atagaatgta caagctttgt atctttatca tctacttcct cttctactgc attgttgtat
    45961 gctgttttac cagcatctac agattgtctt tctctatatt ctttcttact ataaagctta
    46021 acttcttctg aacttcttcc tggtgttttt atatcaaact tcttgattgc ccatgtaaat
    46081 attatataag taactataaa aaatattggt atcaaaaata ataatggtaa cgcatccact
    46141 ttttgtggtt gtaataaatt aggaaacata tttctaaatg attctcccat tatagaaaca
    46201 ttaaacattt ctgtaaatac atacgtcaat cctgccagtg gtacatatgc tacaaaatac
    46261 aataatggtg caacaaatag gaatgtatac tctattggtt ccgttacccc taccaacatg
    46321 gctgttacta ttgctggtat caatatactt attgcttttt tcttattagc attatcagcc
    46381 aacttataca tagctaaagc tgctgctggt aatattgcaa aatgtattaa taaacgacct
    46441 gttgtgaagt tacgagttat ataagatagt gtatctgggt ctcccatttg agctaatctt
    46501 atattattaa caccctcgta aattgctcca cctacttcca ttgttccacc tactcttgta
    46561 tattctatag ggaatgcaat taagtgatgt ataccaaatg gtaataatgc tttatccatt
    46621 gctccaaaag taaatgttcc taataatcca ctagaaccta tccaattacc aagtttagat
    46681 aatgccaacc ctactggtgg ccatataaag tacattgctg tacccaatac tattgatgct
    46741 ataaacatca taatcattga aaatcttgga cctgagaaaa aagataatgc actaggaagt
    46801 tctatgtcaa caaatttgtt atgtactgca ctagctacaa atccagctat tatagctcca
    46861 aatatactac aatcatatga aaagattcct aagaaagaac catataaaga acttcgacta
    46921 tatgcttctg cctctcctaa tccagatgcc ataaacgctt ctgctgtaac tgtatctgaa
    46981 tttataccat ttgctgctaa caatgtactt ataactgtgt gcattcctat aaataaaaca
    47041 attcctccta atgcacccca tcctttttcc tttttagata atccaaaggc tatacctgta
    47101 acaaagaata ttggtaagta tctcattacc ataaatccta aatcgtttat tactttaaag
    47161 aatacattta taaatgaccc ttcttttatt agtccgctaa gccctaatgc agaaacgttt
    47221 tccatatttg caaaagctga ccctaatcct ataaatactc ctgctacaac aagtaatata
    47281 acaggtatca tcattgctcc agaaagtact tgtaatttat ccttcatagt attatttcct
    47341 ccatttttat tgaaatgcca ggctatataa tgcatatata tatacttcta ttgctttgta
    47401 taatgtatct atacttatat tttcatttgc ctgatgtgaa tttggattat ccccctctat
    47461 tgtgggtcca aatgcaactc cattttttaa cactcttgca taagatgcag ctccttttgt
    47521 aaaaagttcg gcttcttttc caaaaccttg tttatatgca tttgataatt tatttattaa
    47581 ttcactatct ggttttacat acagtaattt taaatcctta tatatatcaa ctattaaatt
    47641 ttcttttttt ccaatttcat taattctatt agttataaac tcccaactta tacccttagg
    47701 atatctaaag tctattttca tattcaattc atttttctct aagaagatac tcattataca
    47761 tattgtagaa actcccatat ctacatcttc tttatacatc cctaattttt ttccatgatt
    47821 atcatctgta aagtatgaat ttaaaatatc ccttattata acgcctttat catttagttt
    47881 tttaattttc tctaaatctt ttgctaaact aaatgcacag ttatatgacc tgtgtggatt
    47941 tctcgataaa gccctttctc ctgtatatct tactaatact ttaccttcat ctaattcttc
    48001 tatctctgta taataagcta atgattctac taaatctttc ttatcattag ttttaaaaac
    48061 agcctcaatt ttgtcacata caaatccatc ttctttgttt gactttatat caactaaatt
    48121 gtgatttcta aatttatctt tatctatttt ctttcttaga ttgaagtata aaataccttt
    48181 ttctccattt actattggga agtttccatc tggagaaaat gcaaattttg gctgttcatt
    48241 gacactaaag taatgctcca tacattccca agtcgtctct tcagctccac ccactatcaa
    48301 tctaatcttt ctcttcggct tttcattaag ttctcttaag aaaagtaatg catataaaat
    48361 ccctatcaaa ggtcctttgt tgtcattaac tcctctacca taaagatagt tatctttttg
    48421 acatacttta aaaggtttac tcttccaaag ttctttatta tatattggca ccacatctat
    48481 atgattaagc acccctacta cttcttctcc ttctccatat tctatatgaa ttgcatatcc
    48541 atcaaagtct tttacaacaa aatctttgtc ttttgctatt tgaataagct tatcgaaggc
    48601 atttcttatt tctatcccaa atggttgatt tatatctgtt gtactttcat ctctaattga
    48661 aggtatttca actaaacttc taatatcatt tatgatattt tcttgattgg attttaaata
    48721 atcaataatt agttctttgt ttactatcat cttaattatt tatcacccct ttatattaat
    48781 tttacaaagc atttcagttc atatctacat tataaatttt gaatattatt ttttcaatat
    48841 ttcaaatact tctctaagtt accataatga tacggtaaac aatcaacaga ttatgacaaa
    48901 atcatactat acctattact aatattttaa cttatctatt acgtcttttt taatgtaagt
    48961 ttattccaaa aatacataaa aaaagatgta gccttagcta catcttctta tatctattca
    49021 acttccatta ttatgctatt aatatctaca tttccaggtt ctatcctatc ttttactgtt
    49081 ctcttaacag aatcagcatc atccattata actacaggag ttattatatt taaatcattt
    49141 gacgttataa aatctaaatc tacttccatt attaaatctt tagcttttac cttatctcct
    49201 gcctttacct taacatcaaa cggctttcct tctagtgaca ttgtctctaa acctacatgt
    49261 attaatatat ttgtattatc attactctta atcattattg catgctttgt atcaaataca
    49321 ttgactattt caccatcaac aggagaatat aacttacctt cacttggtat tattgctact
    49381 ccatctccca tcatcttttg ggcaaaaact gcatctggaa catcttctaa gttcactaaa
    49441 tttcccttaa aagggctata tattttctca atcttatttt cttttttctt aaatatatta
    49501 aacataacac actctccaca ttataaaatt ttatgataat atttttctta aagcttttgc
    49561 tacaccatca tttacattag tatcagttac aaaatctgcc atagatttta cttcatctgt
    49621 agcatttccc atagcaatac caagacctgc aaatttaatc atggatatat cattttcatt
    49681 atcacctata caaataactt ctttgctatc tatacctaat atatcacaaa attttttaac
    49741 tgcaatacct tttgaagtac ctttactcat aacttcaaag tttatctttc cagaacttac
    49801 tacttctaaa tcttcttgtt ttttaaattc ttcctttgct tctctcaaag aatctacgtt
    49861 ttctgagaaa agtatacatt ttaaaatttc attatcagct ttttctaata aagtttcaca
    49921 acttgcattt tctattattt caattctgtc ttcttcttta tttttactat ttattaatct
    49981 atatggatgt tcttcaccta tttcattgtt ggatataact attttacagc ctttaaaatg
    50041 tttgtttaaa ttatactttt cacctattgt atagattttc tttagagctt ctttgctcaa
    50101 aaccttctta tactcatatc ccagtaattt aaaatatgtc ccattagttg atataatata
    50161 tatatcatcc cctaaaacac ttgtaaaata tttcatagca ttataaggtc ttcccgtaac
    50221 taaggctacc ttgataccct tttccatggc ttcttttagt gctttaatat tttcttctga
    50281 tacttcaaat cctttaccca tcaaagtacc atccatatca gtacaaatta atttatatcg
    50341 catttgtatc acatcctagt aagatttttc taatatttca acaaggtctt ctttctttcc
    50401 aaaaggtaaa aatcctaaaa ctggacagct tgttatttgt tctaattgtt cctttgttac
    50461 attaaactca gacatttttg tcttaagccc cactctcttt aaaaaacttt ctatttcatt
    50521 tcctaaagtt ttagctgctt catcatcttc cactttttct aattcttggt ttagtattct
    50581 cgctactttg gcaaatttct ctatattatc cttatagaac ttttctgcaa attttgggaa
    50641 cactatagcc aaagcctcac catgaggaat atgtgtaact cctcctatta tttcacttag
    50701 tggatgtggt gctgctgctc cagcatttgc cagtgaactt cctcctaatg tatctgctac
    50761 tgctaacttt gttctgtatt ctatattatt tttatcttcc attactttag gtagatagtt
    50821 aatgactaat tccatagatt tttcagacat tatctctgta aaatcattag catttggatt
    50881 tatataactt tcaaatgcat gagtaaaagc atcaaatgct gttgatgcag taattctctc
    50941 tggcagagtt aacataagct ctgggtcaac tatacattct tttgagaaac aattttgatg
    51001 aaatattgta tttttctcgc ttcctctcga tattactgct gcttgtgtaa cttggcttcc
    51061 tgttccagaa gtagttgata cagctattaa tggtaattct ttttcactta ccttatcgta
    51121 tattttaaat ggatttgtat atgtactaaa catataatcc caatctattt tttctaaacc
    51181 aaaagtcaat gctaatactt ttgcagtatc tatacttgaa cctcctccaa cagctaaaac
    51241 aacgtctaca gattcttttt tagctaattc aaacccttcg tttattaatt ctacagttgg
    51301 gtttggctct actctctgaa cctcccatga ctaaagtcac agggttcctg cttcacagaa
    51361 ttttgcatac taaaatatgt cttactaatt ctccacaggc catccccgta gttcctacgg
    51421 ttcttacata agttatttat tttgaattgt tattagtctt aatccttctt ttaatatatt
    51481 tatacttgca tttatatctc tatcgtgagt ttcattacaa ctaggacaaa tccattctct
    51541 tatatttaag tccttcactt cctcgttttt atacccacat ttattgcata tttgtgaact
    51601 tgcaaagaat ttacctacct ttacaatttg tctaccatgc caattagcct tgtattctaa
    51661 ttgacgaata aattctgacc atgatacatc tgaaattgag cgagatagtt tacgattttt
    51721 aatcatattc tttacttgta aatcttctat gcaaataata tcattctctt ttattaattt
    51781 agtagataat ttttgcagaa aatcatttct ttgattagct atatgttctt gaagtcttgc
    51841 aacttttaat cttgctttat ttctatttaa actaccgatt gtttttctcg ataattctct
    51901 ttgtaattta gcaagtttgt ttaatgactt cttaagatac ttaggatttt ctataaattc
    51961 tccacaactt gagatacaaa attctttaat tcccaaatct aaacctatat gattattagt
    52021 attttcaaat gcttcaatat ctacatcagt acagcataat gatacataat atctaccact
    52081 tggttcttta gatatagtag cattaagtat tctaccttga ggtacttgtt tatcccttat
    52141 cttaaccata ccaagtttag gtagttttat atattgacca caatacataa tgtttccatt
    52201 tgtaaagtta gttttatatg aaaatctatt aattttcttt gatttaaatt taggaaatcc
    52261 tgctttttct ttaaagaatt ttttatatgc attatctaaa tcttttaagg cgttttgtag
    52321 tgaaaatttg tcaggttctt taagccaatt taattctttt tttaaattgg tt
//
LOCUS       DAYAPA010000011        51445 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.011, whole genome shotgun sequence.
ACCESSION   DAYAPA010000011 DAYAPA010000000
VERSION     DAYAPA010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 51445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51445
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.011"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            <1..643
                     /locus_tag="ACGI59_000570"
     CDS             <1..643
                     /locus_tag="ACGI59_000570"
                     /inference="COORDINATES: protein motif:HMM:NF022615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2953 domain-containing protein"
                     /protein_id="HFG9738378.1"
                     /translation="DNINMELNELFNFKYLIIAVIFIISVTMLILISHINILIIADIK
                     NKDICLKLNIKYMFNLININRQLYPAENSKNNDKKEGMKNNIDSSILLADDLLSIYRL
                     LKKIKIHELYSNINFGTGNIGLTSSVYVLINTLYGNLFNMIDAEKMYLNVNPDFTKDY
                     VLGNIRIHIRPRIKALFNIIIMINKIMNKNKGNKEGDSNESNRFDTESYGNNS"
     gene            597..1025
                     /gene="ytfJ"
                     /locus_tag="ACGI59_000571"
     CDS             597..1025
                     /gene="ytfJ"
                     /locus_tag="ACGI59_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GerW family sporulation protein"
                     /protein_id="HFG9738379.1"
                     /translation="MRATGSIQSLMETTLETIKGSIDANTIIGDPIKTDTTVVVPISK
                     VTIGFGIGGGEYSKGYDDKDREIELKNEKSDTNFAGGSAGAISVQPVAFVVVESGETR
                     IMSLDSNINLVDNILSITPRVLEKIQNISQSNKNQDKNNM"
     gene            1273..1713
                     /locus_tag="ACGI59_000572"
     CDS             1273..1713
                     /locus_tag="ACGI59_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFG9738380.1"
                     /translation="MSDKELSILLLESMNNFHNLIKIINNERYKKDKVLTERQFFALV
                     KIRKHDKIELKNLSRDLHVSTSSLCILLNKLVEQEYVYREEDSRDRRNTFYGITKNGE
                     KILDNEILKFVSIISDKMDCLDINNKDKLFTSLEESKNIIEQLF"
     gene            1729..3180
                     /locus_tag="ACGI59_000573"
     CDS             1729..3180
                     /locus_tag="ACGI59_000573"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HFG9738381.1"
                     /translation="MNIKYSKEILKSLENAITVVALFEGEDVSTTFDKINMISRNFNS
                     KENSIDTVTLVENNNLVNVCLVGFGKRELLNREKIRIIGGNLCKKLKNILKNEAYKSS
                     DVNVLNLNLNSDEIGCFVEGILLGNYKFDKYKTKSKDFERKEVDTITIFTEKEVECQI
                     EKAKILANSTIIARNLVNEPSNVIYPKTLAVETVKLGAEFGFNVDVYEEEKIKALGMD
                     AFFAVSRGSINKPRFIVMRYFGDKESTDILGLVGKGLTYDTGGYSLKSNASMLDMKTD
                     MAGAASVIGAMCAISQSKLKKNVIAVVAACENALSGGSYKPGDIISSMAKKTIEVLNT
                     DAEGRLTLADAIYYIINNEKVTKVVDVATLTGAALILLGNVATPIVTNNDDFYCELEK
                     AATLSGERVWKMPIYDEFKDMIKGEEADLKNTGGKNAGCITAGAFIGEFVGNTPWIHM
                     DIAGTSTSSKSIGYKAKGATGEPVRTLYYLAEI"
     gene            complement(3271..3939)
                     /locus_tag="ACGI59_000574"
     CDS             complement(3271..3939)
                     /locus_tag="ACGI59_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwaF family protein"
                     /protein_id="HFG9738382.1"
                     /translation="MFKIFLFSSEQFVSLFIFGLFLYYCPKLTKNILPYSYTVEKIIC
                     TLLVIIMALEQLLLISSGNYSTLNSLPIGINYICIYLCIAILIFKQYHLFNIFFSWSL
                     VCSVGELIFSKNLGYEFPSLIYFIFIFSKCLIIYADIYMVDVRKFRVNRYALRDNLAI
                     CFIYFSFIFLLNTFTNSRYYYGFLSHSTTAIFTFIFVTSIMYIPALLFNRDTFILEKK
                     KKSK"
     gene            4208..4348
                     /locus_tag="ACGI59_000575"
     CDS             4208..4348
                     /locus_tag="ACGI59_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9738383.1"
                     /translation="MGIIESASKLAEMVHLLAVEKGITDIEAWDEAVKEYSKIYEERR
                     NE"
     gene            complement(4523..4843)
                     /locus_tag="ACGI59_000576"
     CDS             complement(4523..4843)
                     /locus_tag="ACGI59_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HFG9738384.1"
                     /translation="MKLKTISLPELNNLDPTLESTFIKMGEEQGELAECIGKFRNLSG
                     ENNDLDEVDIIKKTAKELMDVAQTCVTMMFKLEEQYGINLDEIRKEHIKKLEKRGYIK
                     NMDK"
     gene            complement(4963..5700)
                     /locus_tag="ACGI59_000577"
     CDS             complement(4963..5700)
                     /locus_tag="ACGI59_000577"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein lysine
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HFG9738385.1"
                     /translation="MDANSLKDLIANHNNIVFFGGAGVSTESNIPDFRSSTGLFSQKL
                     NKQFTAEQLVSHTFFVRYPEEFFEFYKDKLIYPNAKPNNAHIALAKLEEMGKLKAVIT
                     QNIDGLHQMAGSKNVLELHGSVHRNYCTKCGKFFDLESMLNLGGNIPYCDNCGSIVKP
                     DVVLYEEALDSDVITKTISAISNADLLIIGGTSLAVYPAASFIDYYKGDYIALINKAN
                     TVYDKSASLVINKPIGEVLYEAVLRQI"
     gene            5915..7738
                     /gene="acd"
                     /locus_tag="ACGI59_000578"
     CDS             5915..7738
                     /gene="acd"
                     /locus_tag="ACGI59_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosaminidase"
                     /protein_id="HFG9738386.1"
                     /translation="MKKKAALATLAMLPLGVVNAHADGDIGIVTINYLNVRNEPTAES
                     SIAFVAKKDDKVLIKDSSNGWYKIKAESGQEGWASSKYIAKSNSDSLRTSTNKEKQVI
                     SNSLNMRNGAGTSYRVITVLKKGQKVEVISESNGWSKIKYDGRLGYVSSSYLGDVSNS
                     TNKSKTKQVNTTSLNVRSGPNTSYGLLGKLPKGSKVEVISEGNGWSKIKYNGKDAYVS
                     SMYLSDVSQSNSDNSSQSNDKKNTDKVVNTASLNVRSGPGSTYSKLGKVYKGSKVTVL
                     SESSGWAKINFNNKEAFVVGNYLSTSADTSNNNSNSNSDNSSNSNGNNSSSSGQVNGM
                     SGISGAKIDYKSLSYTLESHISKQVEKAASGGNVIAPSNRKSTPSPEFSTFSAQRTSS
                     FVNASSSDIEYYLNPKNFTNTTKGMMQFLKINSYRDGISESSLNSYLNGLSSSVFKNQ
                     GAAFINAAKKYNIDVVYLVSHAMWETAYGKSTLAQGQTLTSYKGQPLSKPVKVYNFFG
                     IGAIDKSANVSGAEAAYSNGWTSVEATIDGSAKWISQNYVNSSKYNQNTIYKMKWNYD
                     YTWHQYATDVNWANGISGIMENLIGLYGGGSSLVFEVPQYK"
     gene            7953..12251
                     /locus_tag="ACGI59_000579"
     CDS             7953..12251
                     /locus_tag="ACGI59_000579"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HFG9738387.1"
                     /translation="MESIKEYLDKLEINNSGLGKQLKEVYINRVVYFKEDKIVYFYLT
                     SKDIVSHELLDKFKEELMYKLDYFKDMRMKIRFTGLERKSNKDVIKKYWNNILYILKY
                     LCPSIAGWYKQVEFLCLEEELKIKLPKGIFYERLMKKNVTYVLKTVLSEELGLDLNIT
                     IEKAVDEKVNKERLIRINDREMEEKIRALEIGKVNNCEENEEESYVIKSDVDENLIYG
                     DNANAMIENIVELNASSGTVAVVGDIFDVDTKELKNGKILMIASITDYTSSISCKLFL
                     TDTNKDGVLENVKKGAYLKIKGDIVYDTYQREISMMISGIRKETRIEREDKSEEKRVE
                     LHAHTQMSSMDAICSVKKLVERAAKWGHSAIAITDHGVVQGFPDAMNAGKSNNIKILY
                     GVEGYLVEDDSLIIEDANDKELSQTFVVFDIETTGFSNTNDKITEIGAVKIENFKIVD
                     RFSELINPEKDISYKIQELTGITNELIKDKPTIEEVLPKFMEFVGDSVLVAHNAEFDT
                     GFISQKCREQGLLYNNKKVDTLMLARVMLPNLKRHRLNVVAKELGIPLLNHHRAVDDA
                     EATALIFNKFLQMLTEKGAKTLSDVNNILGKIDYTKLSTNHITLIAKNSIGIKNLYKI
                     ISDAHVNHFFRAPRILRSVLNEYKEGLIIGSACEAGVVFQAVKKNVSDEEMKKIIDLY
                     DYIEVMPIDNNRFMIDKGEVKDEEELRELNRKLIDTAKKFDKIPVATGDVHFLDKHEA
                     VLRKVLKYSQGFKVDEEETYLHFRTTDEMLEEFSYLGEELAYEVVVENSNLIADMVED
                     IKPIPDDTYPPIIEGSDVELREMCYKKAKRIYGDPIPEIVQKRLDRELNSIISNGYAV
                     MYIIAQKLVAKSLSDGYLVGSRGSVGSSFAATMSDITEVNPLPAHYICENEDCKYSFF
                     YEIGEWGSGVDLPDKDCPKCGRKLAKNGHDIPFEVFLGFEGDKEPDIDLNFSGNYQPT
                     IHKYTEELFGEGYVYRAGTIGTVAEKTAFGYARKYVEENNISVPNAEVLRLSNGCTGV
                     KRTSGQHPGGVMVIPDYKDVYDFTPIQYPANDTSCGVITTHFDYHSISGRILKLDILG
                     HDGPTIIRMLEDMTGINITEIPLDDKETMSLFTSTEALGVTPEEINCPIGCLAVPEFG
                     TKFVRQMLLDTKPTTFAELVRISGLSHGTDVWVNNAQDLVREDIVGLKDVISTRDDIM
                     NYLIFKGLPPKMSFTIMESVRKGKGLKPEHIEEMKKNNVPEWYIGSCKKIKYMFPKAH
                     AVAYVMTSFRIAYCKVHYPEAFYATYFTTKVEDFDADLIVKGLDAIKSKINEIESLGN
                     DATTKEKGMLTVLEVALEMYARGIKLLPVDIYKSDATEFIVVGEKTLLPPMAAIQGLG
                     GNAAINIQNERKNGEFISKEDLRKRTKISKTVIETLTNHGSLENMSERNQLSLF"
     gene            12455..12916
                     /gene="rimP"
                     /locus_tag="ACGI59_000580"
     CDS             12455..12916
                     /gene="rimP"
                     /locus_tag="ACGI59_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFG9738388.1"
                     /translation="MKKNLEATIEEIVTKITDEHGFEMVDVEYVKEAGEYYLRVYIDK
                     EEGISLNECELVSRELSPILDEKDPIKENYFLEVSSPGLDRALKKDRDFVRYQGRDVD
                     LKLYKPLNGCKQFEGELVGLTEDNNIKIIANGKEMEFNRKDVAIVRLAIKF"
     gene            12946..14100
                     /gene="nusA"
                     /locus_tag="ACGI59_000581"
     CDS             12946..14100
                     /gene="nusA"
                     /locus_tag="ACGI59_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFG9738389.1"
                     /translation="MNHEFMEALDELVVDRGIDKEILIDTIEQALLTAYKKNFGSAQN
                     VRVEFDRDKGDIKVFSQRVVVDESDLYDTFLEIELADAREINPNYELGDIIEHEVTPK
                     NFGRIAAQTAKQVVVQRIREAEREIVYNEFIEKENEIVTGEVARANKNVVYVNLGRIE
                     GIMTQSEQIPFENYKAGQKIKFYILEVKKTNKGPQIVVSRSHPGLVKRLFEEEVPEIY
                     EGIVQIKSVAREAGSRTKMAVRSIDEKIDPIGACVGPKGLRVKNIVDELGDEKIDIIK
                     YSEDPAEFISAALSPSKVVRVDIDEDEKSALVIVPDYQLSLAIGKEGQNARLAAKLTN
                     WKIDIKSESQAEQEMLNLENSKEIEAEDVEAEFIDQEELDTSEDNFSEEE"
     gene            14107..14388
                     /locus_tag="ACGI59_000582"
     CDS             14107..14388
                     /locus_tag="ACGI59_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HFG9738390.1"
                     /translation="MILKKVKKVPQRKCIACQDRDSKKELIRIVKNKEGKIFLDPTGR
                     ANGRGAYVCKSSECLKKAIKSKALNKAFKIDVPDEVYDNLLMELEEYEK"
     gene            14378..14689
                     /locus_tag="ACGI59_000583"
     CDS             14378..14689
                     /locus_tag="ACGI59_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L7Ae/L30e/S12e/Gadd45 family ribosomal protein"
                     /protein_id="HFG9738391.1"
                     /translation="MKNNKEKVLSFLGLATRAGKIVSGDDSTLLDLKKGKVNLILIAE
                     DASNNTKKLFKDKSTFRNIPYLFFSTKEEIGFAIGKSPRAVVGIKDENFSKKIIELIE
                     I"
     gene            14711..16651
                     /gene="infB"
                     /locus_tag="ACGI59_000584"
     CDS             14711..16651
                     /gene="infB"
                     /locus_tag="ACGI59_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFG9738392.1"
                     /translation="MSKTRVYQIAEELNISNEELINKLAELDINVTDKDSVLEGEELE
                     LALEMLGEDLSQENGNVIEIDGKLTVQVLATKLDKSPSEIIMKLMKMGTMATINQEIS
                     FEIAALAAKDYGFELTVAESDDTEALEIEALMEIEEDKEEDLKPRPPVVTVMGHVDHG
                     KTSLLDAIRKTDVISGEAGGITQHIGASEVKINGHKIVFLDTPGHEAFTSMRARGAQV
                     TDIAILVVAADDGIMPQTVEAINHAKAAGVPLIVAINKIDKPGANPDKVKQELADQGL
                     LVEDWGGEVIAVPVSAKKKEGIDTLLEMVLLVAEMEELRANPNKRAVGTVIEAELDKG
                     RGPVATVLVQGGTLTVGDPIVAGVACGKVRAMINAKGKRVKTAGPSTAVEILGLSEVP
                     QGGDQFVEVPTDKIARSVAARRQQIVRDEMLKSTQRLSLDALFSQMSEGSIKDLNIVI
                     KADVQGSVQAVKQSLEKLSNEEVQVKVIHGGVGAVTESDILLAAASNAIIIGFNVRPV
                     PGAESLGEKENVDIRTYTIIYKAIEDIQAAMTGMLDPEYVDEETGKAEIREIYKISGV
                     GTVAGCYVTNGKIFRNCKVRLVRDSIIIHEGELAALKRFKDDVKEVNSGYECGMSFVN
                     YNDIKEGDIVEAYITKEVERKL"
     gene            16694..17074
                     /gene="rbfA"
                     /locus_tag="ACGI59_000585"
     CDS             16694..17074
                     /gene="rbfA"
                     /locus_tag="ACGI59_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFG9738393.1"
                     /translation="MASYNRTRRIAEEIRKVVSTMLINGVKDPRITSMVSVTDVEVTN
                     DLRYAYVYVSILGGDEESTLTGLKSAGGYIRREVGKNIKLRYIPEIVFKLDDSIEKGM
                     YMDSLIKRVNEKNAQQNEDENYDE"
     gene            17055..18038
                     /locus_tag="ACGI59_000586"
     CDS             17055..18038
                     /locus_tag="ACGI59_000586"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HFG9738394.1"
                     /translation="MKIMMNNMDSIINYIYEGDYFIVTSHISPDGDNIGSTLSMYYTL
                     KNLGKNVYYVLDDEAPLNLRFLVEDVKIYKSNEFNMKNYSLIALDCGDKMRICVSDEI
                     KNNASKLICIDHHASNDSYGDLNYVDIDASSTCELVYNLLVRFQQIKDINIINEDIAT
                     CLYTGLVTDTGNFSYSNVHASSFEMAKNLLVLGAQKNTIIQNIYQSNSSGYYKLLGEA
                     LKGLEIFDSKVSSIVLTQDMMNRNNISFNDVDGITTYTRDIKGIEVGILFKEKKQNEI
                     KVSFRSKNYVDVSEIAKLFGGGGHVRAAGCTIRDSIDNAKKMVLEAVLKSI"
     gene            18051..18956
                     /gene="truB"
                     /locus_tag="ACGI59_000587"
     CDS             18051..18956
                     /gene="truB"
                     /locus_tag="ACGI59_000587"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFG9738395.1"
                     /translation="MNKIISILKPTGMTSHDVVSRVRKILNIKKVGHTGTLDPDASGV
                     LPICIGKATKVCEVILNKDKSYICELTLGISTDTYDASGEILKKVDDFKFSNEDIERA
                     FDTQRGEINQLPPIYSALKVNGKRMCDLVRSGRQSEITLKTRRVNIKDIKILSIKGNK
                     VMFYVECSKGTYVRSICHDIGEYLGCGAHMSFLNRTSSGKFDLDNSITLEELELFYEN
                     KTLDKYLYDIDYVLDSFNYVVLNPNAIKYYSNGGSIDDKRFLKNNFDKDDEFVRVYST
                     DNFLGLGKLSKHNNTISVKSDKMFI"
     gene            19087..20016
                     /locus_tag="ACGI59_000588"
     CDS             19087..20016
                     /locus_tag="ACGI59_000588"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HFG9738396.1"
                     /translation="MVIIKSIKEIANIKESVITIGNFDGVHKGHQVLIGKTVEHAKKE
                     NIQSIVFTFANHPVNYFRPNSTKNIISNDDKIKLLEDLGIDIVVIIPFDEYMTKIPAK
                     DFVDEILVKKLKAKKIVIGHDFTFARAKEGNVNLLKSLEHEFGFEVEVIKPIKINDVR
                     VSSTYIRSLVSQGDMANVKEYLGRNYKLEGCVIHSKHLGRTIGFPTANIDLKNNMLVP
                     KRGIYASIVHIGDETYFGATNVGYNPTVNGKTLSIETNILEFDRDIYGENIIVEFLER
                     IRDERKFNSIDDLKNQLYNDTNYVYENYVCKNK"
     gene            20120..20377
                     /gene="rpsO"
                     /locus_tag="ACGI59_000589"
     CDS             20120..20377
                     /gene="rpsO"
                     /locus_tag="ACGI59_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002843739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFG9738397.1"
                     /translation="MINKSEIIKEYGRAEGDTGSPEVQIALLTARINELNGHLKTHKK
                     DHHSRRGLLKMVGRRRNLLAYLKEKDLEGYRALIAKLGLRK"
     gene            20617..21462
                     /locus_tag="ACGI59_000590"
     CDS             20617..21462
                     /locus_tag="ACGI59_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFG9738398.1"
                     /translation="MKIKLVCDSLCDIPDEISEKDYIEIVPLTVIFNDREYIEGVDIK
                     KEEFYKKVKEIKQIPKTSQATYMEFKEVFDKFITEGYHIICMTGAANASGTFQSAMIA
                     KNDVNENEKIHIFDTRNLSLGSGQYVIKACELLEEGLGFEEIIDELENTRSSVRLLFA
                     PYTLDFLKQSGRVPVATALIGNMLNIKPIFFFDNGEAKLVNKVRGIKNIASKLVDIIL
                     EMNEGSLEGKIVTIGCGDNLHDCEILKDEVNKRIKARRVLFTRGGVSICSHTGPDILA
                     ISCSN"
     gene            21587..23698
                     /gene="pnp"
                     /locus_tag="ACGI59_000591"
     CDS             21587..23698
                     /gene="pnp"
                     /locus_tag="ACGI59_000591"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFG9738399.1"
                     /translation="MFEHKIFKMDFAGRELSVEIGKICEMASGSCIVRYSDSMVMVNT
                     TKSAKPRDGIDFFPLSVDYEEKLYSVGKIPGGFLKREGKPSEKAILTSRLIDRPIRPL
                     FPKGFRNDVQVVATVLSVDQDCTPDIVAMIGSSIALSISDIPFNGPTGSVCVGLVDGA
                     FVVNPNAEQREKSSMHLVVSGTKEAIMMVEAGADEVPDEVMLDAILFAHQEIKKIVEF
                     IEGIVAEVGKEKMPVELYHAGEEITQLVREFATDKMKKAVQTFEKLERMENMDRVKEE
                     TLAHFEETLEDFEDFVGDIEEVLQDIIKEEVRKLIVHENVRPDNRKLEEIRPIWCETG
                     MIPRAHGSAIFTRGQTQVLNVATLGALGDVQKLDGLDEEENKRYMHHYNFPAYSVGEA
                     RPSRGPGRREIGHGALAERALLPVIPSQEEFPYAIRLVSEVLSSNGSTSQASVCGSTL
                     SLLDAGVPIKDMVAGIAMGLIKHDGKVAVLSDIQGMEDHLGDMDFKVAGTEYGITAIQ
                     MDIKIDGIDKEILQRALKQAKEGRIHILGEMRKTISQPKPELSPYAPKIVKMQINPDK
                     IKDVIGPGGKIITKIIDETGVKIDIEQTGEVFISGIEIDMIKKAQELINNIVVEPEVG
                     KTYKGKVSRIMNFGAFVEILPGKEGLLHISHIAHERVAKVEDVLNIGDEVEVKVTEID
                     EKGRVNLSRKVLLPKPEHKNK"
     gene            23964..24707
                     /locus_tag="ACGI59_000592"
     CDS             23964..24707
                     /locus_tag="ACGI59_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFG9738400.1"
                     /translation="MIMMVLNKSKLKKIIILILVVLLVLSGGVMVLKLFNNKPVFNLF
                     NGVDLPYERGTKDKSGYVALTCNIDLGWETEYVESILETLKKENVKITFNVTGKWAEK
                     NKDELLKIKKQGHEIGNHGYKHLDYSTLSYEDNYEQIETSKKIIEEIIGEKTKFFQAP
                     AGSFGPETVKAAKALGYTSIKWDADTIDWKYKDQPEVIIDRMKKKDIKDSSIILMHPT
                     NATTKCIDDIIAIVREKGLKAGKLSDVFK"
     gene            24865..26112
                     /locus_tag="ACGI59_000593"
     CDS             24865..26112
                     /locus_tag="ACGI59_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFG9738401.1"
                     /translation="MYKTKILENGLTIIGEEIPYLKSITLGIWINAGSRIEEAQVSGT
                     SHFIEHMMFKGTKNRTSKEIASSIDNLGGQINAFTSKECTCYYVKLIDEHIDTGIDVL
                     SDMILNSKFDKNDIDKERLIILEELKMYEDSPDDLSYDLLVENIYANDGLGMNIIGTK
                     ESLYNITRESMLEYLNKYYIPNNAVISIAGNFNFDDMVEKIKSKFGHWEKKNLSIDIS
                     EAKFNPCFISKNKDTEQVNLAMCLKGIPFENDEEVYSMAVVNNIFGGSISSRLFQKIR
                     EEKGLVYSIYSSQTLYRKCGELGIFASMSTENLQDVYNLIKKEIENIRENYLTEKEIS
                     ESKEQLKGNYILDLESTSSRMMSTGKSMLLSKKVKTTDEILECINSVNINSIKKVVDK
                     VFNIENIGTCIVGRDVEKILHLD"
     gene            26147..26392
                     /locus_tag="ACGI59_000594"
     CDS             26147..26392
                     /locus_tag="ACGI59_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HFG9738402.1"
                     /translation="MNLSEIAGKEIVNLVTGERLGVIGECDLILDETTGGILALLIPK
                     EKGFFARRKDKSFLEVPWRNVKKIGNDMIIIEYEGYI"
     gene            26402..27625
                     /gene="dapG"
                     /locus_tag="ACGI59_000595"
     CDS             26402..27625
                     /gene="dapG"
                     /locus_tag="ACGI59_000595"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HFG9738403.1"
                     /translation="MSILVQKFGGTSVESYEKMNEVCKIVKAYKKNDEELQLVLVVSA
                     MGRKGAPYATDTLINLCSAVNDEPSKRELDLIMSCGEIISGTILANLLNAQGIDSVFF
                     TGQQAGIITSDEYSNAKIKYINPKKVKRALDDGKVVVIAGFQGVTDDGEITTLGRGGS
                     DTSAVAIGKALECETVEIYTDVDGIMTADPRVEPNAKVLSFIDYEEVFQMADKGAKVI
                     HPRAVQLAKSGNITLAIKNTMNPTFEGTKIGSLCRHLEDNIEYEQERDFKVAVANKDS
                     VAQVKIKSAEEVFTEVLNEIEKKLITIDMINFFISEKAFVVEDADIKSLKEILDKFEL
                     DYEVNRDCAKVTLICSRIADEMSGIMSKVVRGLSKAGVSLLQTSDSNMTISCLVSEED
                     MHTAVHAIHQQFYLK"
     gene            27738..28472
                     /locus_tag="ACGI59_000596"
     CDS             27738..28472
                     /locus_tag="ACGI59_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HFG9738404.1"
                     /translation="MKDYREDNERFDKDNCSEESSCEERECIKQFGTNEMPPQPPKDI
                     QCITIIGEIEGHFIGNPQKKATKYEHIIPMLYSIEESNDVKGVLVVLNTVGGDIEAGL
                     AIAELLNSTSKKVVTLVLGGSHSIGVPLATAGDYSFIAPTATMIIHPVRTTGLVIGIN
                     ETFEYFKKMQDRIIQFIIRTSNIKKDVLEKLMHEKDELVSDVGSVLIGKEAVDYGLID
                     EVGGLKEALKKLRELIKESEEEKNND"
     gene            28595..31006
                     /locus_tag="ACGI59_000597"
     CDS             28595..31006
                     /locus_tag="ACGI59_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFG9738405.1"
                     /translation="MATKKKKKKKVNTLSFNAEYHNLITIFIGVFLLYSLNSNSMGWI
                     PVLMQNLFKGLFGGLSIAIPFIVIITGLLGFFDGNEYIYRLRKTKLYYIIILFIFVFY
                     GLLNAGTLPVDSPLKGNMFDDVMKLGVSGQGSGLIATTIAYYMSKIFGIAGGWLISIF
                     ALILSVMFIFNISIKDLVSNAKSKSKASKDSNLTFKDKIANMKKSAIDMMTDEVDDTT
                     INKKPGFFKGLMSKGLNNDDEEDEYFEEENSDGVDDKTIKIVGFNKAEDEYLEILEGT
                     QSMPELDVLKELQKATNENPVVDTKPEKKVDIAKPNLNIEKTQPMSIVAEPVNEDYSN
                     YKKPSIELLNKVNKKSDENGKKKVLKNASLLEKTLSDFGVEAKINQVTVGPTITRYEI
                     QPSPGVKVSKIVNLTDDIALSLAAKSIRIEAPIPGKSAIGIEVPNEEAQMVGVREVLE
                     SEEFNNFNSPLAMGLGKDVAGKIIIGDIGKMPHLLIAGSTGSGKSVCVNTLISSILYK
                     ANPDEVKLLLIDPKVVELANYNGIPHLLIPVVTDPKKAANALNWAVTEMNRRYKLFAD
                     AQVKDVTSYNEKAEEKLPKIVIIIDELADLMMASANDVEDYICRLAQMARAAGMHLIV
                     ATQRPSVDVITGVIKANIPSRIAFAVSSQTDSRTILDMGGAEKLLGKGDMLFYPLGAA
                     KPVRLQGAFISESESEKVIDFVKSQVKDGIKYEEDIIETISKVNTSKGSDEDEFLSEA
                     IEFVVESGQASASMLQRRFKIGFNRAARLIDSMEERGIIGASEGSKPRKVLISKQDLQ
                     NLEGE"
     gene            31009..32067
                     /gene="mnmH"
                     /locus_tag="ACGI59_000598"
     CDS             31009..32067
                     /gene="mnmH"
                     /locus_tag="ACGI59_000598"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HFG9738406.1"
                     /translation="MSVIKIEKALKLDKVIFVDVRTEGEYEEDHILNAINMPLFKNNE
                     HNDVGTIYKMQGKHEAIQKGFDYVSYKLKDIYLQAAELALNYDNIVIYCARGGMRSGS
                     IVNLLSSLGVNVYQLEGGYKAYRNFVLEYLRHVMDTKNFVSVHGLTGTGKTDLLHLLD
                     EKGIDVLDLEGIAKNSGSTFGFITFDKKPPSQKNFETKLFESIFFSKENYIFVESESK
                     RVGSVTVPNEIFEAMTREGHHILLECDIENRVDRLCRDYIYDKDEGNILILKECINKF
                     RKRLGNKVVDDYIDLLESGEYKELVKRYLLEYYDPLYMHSVEKYKYSKIINFNDTQKA
                     LEDVIDLYESILEGESEC"
     gene            32061..33395
                     /gene="rimO"
                     /locus_tag="ACGI59_000599"
     CDS             32061..33395
                     /gene="rimO"
                     /locus_tag="ACGI59_000599"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HFG9738407.1"
                     /translation="MLKIALESLGCSKNLVDAEIMMGILNNKGYKLIGDFEEADVIIV
                     NTCGFIESAKQESIDTIINFAELKKTGNLKLLIVTGCLAQRYSEELKTEIPEIDAIVG
                     TGSYQNIDKILKELSEMHKIVSLNDIEFVFNEDLPRYISTPSYMAYLKIGEGCSNNCT
                     YCIIPKLRGKYRSRKFEDIIKEAKKLAESGVKELVVIAQDTTKYGFDLYGKERLSELL
                     EELAKIDGFKWIRVMYSYPESITEELIQVIKKYDNICSYFDMPIQHASNNILKLMNRK
                     TTKEDILNKINLIRSNIPDAILRTTIIVGFPGETEDDFKQLVDFVEEVKFDRLGAFAY
                     SREEDTPADRLPNHIDEEVKIQRRDTLMMIQQKISEELNDKKIGKTYEVLIEEQIEDN
                     VYTGRTQGDAEEIDSIVYVKSVDNLEVGEFVSVQINDAMEYDLMGDVLYELA"
     gene            33382..33924
                     /gene="pgsA"
                     /locus_tag="ACGI59_000600"
     CDS             33382..33924
                     /gene="pgsA"
                     /locus_tag="ACGI59_000600"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFG9738408.1"
                     /translation="MNLPNKLTLFRIFLIPVFVLIMLLNIPNKFLIACIIFIIASITD
                     ALDGKIARKYNLVTDFGKFMDPLADKLLVISALTCMIEDHLVSSWMVIIIVARELTVS
                     ILRAIAAADGKVIAAGNSGKLKTITQMVSIVFLLLGAQFENVLILNIGEILILIATLL
                     TLYSGWEYLYKNKELFMSSK"
     gene            34212..35258
                     /gene="recA"
                     /locus_tag="ACGI59_000601"
     CDS             34212..35258
                     /gene="recA"
                     /locus_tag="ACGI59_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFG9738409.1"
                     /translation="MSVDQEKLKALNEALGKIEKDFGKGSVMKLGEATSMSIDVISTG
                     AIGLDIAIGIGGLPRGRIVEVYGPESSGKTTVALSCVASAQKDGGIAAFIDAEHALDP
                     VYAKALGVDVDNLIISQPDTGEQALEIAEALIRSGAIDIIVIDSVAALVPKAEIDGDM
                     GDSHVGLQARLMSQALRKLTGSIKKSNCVAIFINQLREKVGIMFGNPETTTGGRALKF
                     YSSVRLDVRKIDTIKQGDKVIGSRTRVKVVKNKVAPPFKQAEFDIMYGEGISKIGDLL
                     DIAADVDIVKKSGSWYSYNDTKLGQGRENVKKFLEDNLDLTTEIDEKVRAFYNLNEEH
                     EESGTSVSKEIVEE"
     gene            35434..36975
                     /gene="rny"
                     /locus_tag="ACGI59_000602"
     CDS             35434..36975
                     /gene="rny"
                     /locus_tag="ACGI59_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HFG9738410.1"
                     /translation="MDVIDSVVMIVIGAAVGIIAGYFVRKNISEAKIGQAENLAKEII
                     DKAHHDSETVQKEKLLEAKEEIHKWRTEAERENRERRTEVQKYEKRVVQKEEVLDRKL
                     QNLESKEVNLSEKLKVVEKKEEEVEVIKTQQLEKLESISGITSDKAKEIILTNAERDV
                     RREMSIMIKEIESQAKEEADKKSREIIGYAIQKCAADHVAETTVTVVSLPNDEMKGRI
                     IGREGRNIRTLETLTGIDLIIDDTPEAVILSGFDPIRREIARIALEKLIADGRIHPAR
                     IEEMVEKARKEVDNIIKEYGEQAAFETSVHGIHPELIRLLGRLNYRTSYGQNVLKHSI
                     EVAHIAGIMAAEIGADIRLAKRAGLLHDIGKAVDHEMEGTHVEIGMDLLRRYKESKEV
                     IHAMSTHHGDYEPQTVEAVLVTAADAISAARPGARRETLEAYIKRLEKLEEIANSYEG
                     VEKSFAIQAGREIRIMVKPEAINDEEIHLLARDMTKKIEDELEYPGQIKVSIIRETRA
                     IEYAK"
     gene            37289..39625
                     /locus_tag="ACGI59_000603"
     CDS             37289..39625
                     /locus_tag="ACGI59_000603"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFG9738411.1"
                     /translation="MKKNTLLHTNIMVCIIITLGFVATSIIGYQSNTNIFKNDIEHVS
                     TLAAEGIYYQIDKLLSEPINVSLTMANDSLLKNFLDGEKEHLNDEEFIYKLQDYLNVY
                     RNKYSYDSVFLVSTKTNNYYHFNGLDRNLSANNSENQWYYTFLKNDDEYSLNVDNDEA
                     SNNSITVFVNCKIKDDNGATMGIIGVGLKVNSLQMLLKGYNDKFDVVARLIDDKGFVQ
                     LAVDKTGHENINFFENSSSDLSNSKQLILNNKKEQKSFWYSSEKSKSYIVCQYIPSLK
                     WHLVLENDFTLMIKQLHLQFFKGIAVIVLIIIFVLFTITHVLKKYKNQIIKLSVSQKL
                     EYQKLLSKSTEGLYENIFEIDITNNKAGCEDENRYFHEFGLGLNTPYDEALHTIAKNQ
                     IKEEYAQGYLDTFLPENVIKRYNSGINNISYDFLIKKDEESYHWIRINARIFFWTSDE
                     SVRMISYRKNIDEEKNRELLLLERSQRDSLTGLYNKATTEEFIRSFLKNESNQDLEHV
                     FLIFDIDNFKNVNDKYGHVFGDFVISEFALELKSQFREDDIVGRIGGDEFVVLIKDFN
                     NNNYVLIDKLERFCSKINQKKFVEQAEFSIACSIGVAMYPEHGYVYSELYEKADQALY
                     YAKSHGKNSYHIFSEDSIDSISFHIDSRDIKVLLEMATDGVVKFACMENLKLLYFNQK
                     MLEFTGYSAQTVTSIGFDIFSPVLQDDLQMVLDTLREAISKRETFEIVFRFCHTDGHI
                     FPVRMQGKFVSELYQNRYPVFYAIYTDISSINTLLENK"
     gene            40007..41788
                     /locus_tag="ACGI59_000604"
     CDS             40007..41788
                     /locus_tag="ACGI59_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFG9738412.1"
                     /translation="MSQYININSQLDALKLKEFEKIFYENINEDVKVVPKVTPFKGIN
                     TDLLYVKDGRILFIKFMDTTEDIFFILEEELLEVMNEEYELLKLKMGQKNRNISYNYV
                     YIMPYVEVEETYEFEEFVNNNIIDKNKLQDIMNKGSLEEYLNDENDEINLNLFLLDIC
                     SEYYIINDKLHLNEKFKKISFYNDDYKYTATMMEDVQIKDVVSIKYGNTLIEGGSGIG
                     KTAIMLSRAIKLAKVYPHHKLVIFTHTKQLCNELRERIELLYKDNNNLEVHTFSSFIF
                     KLAKKFNLIVDYNMLKNDYEKTFNNLVKQAQNIIKNKNMFKAIFIDEAESFLEYEIDF
                     IREFLYKTKFIFNVCSCNSLNISNRLNIFKKLYNGIEFDDKIILSKNYRQAKEIVDFT
                     NKFSNNSNSYINELRPNTEFSTFFYTKALRGGNKSVDIIKVSDLDDQISSVIWEIEYL
                     ISKKGLDYSEVAIVYPYNKKKLKSGKTIYFQYMLKKALEEAKIPYICAEDNLTNISKK
                     VGTTIANIYAIKNLEYKAVIVCELEMLYNQTINDIEQDYQVNDFVGDLNKVYLAMSRA
                     TDYLSIVTTFNEEASDIIRLITESKDI"
     gene            complement(41870..42400)
                     /gene="lepB"
                     /locus_tag="ACGI59_000605"
     CDS             complement(41870..42400)
                     /gene="lepB"
                     /locus_tag="ACGI59_000605"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFG9738413.1"
                     /translation="MSVKKEIFDWIKSIAMAIVLAFVILQFIRPSIVSGESMYPTLDD
                     KDYLILNRISYKVGKPEKGDIVVFKTNLVDGETGKKKDLIKRVIATEGDRIKISNSKV
                     YVNGKLLNEPYIHNNYTSGDIDTVVPKGKLFAMGDNRENSNDSRFPDVGMVDEDEVLG
                     KVMVRLLPLDNIGKVD"
     gene            complement(42440..43264)
                     /locus_tag="ACGI59_000606"
     CDS             complement(42440..43264)
                     /locus_tag="ACGI59_000606"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFG9738414.1"
                     /translation="METFSLLEIFKAVILGIVQGITEWLPVSSTGHMILVDEFIKLNF
                     SNTFISTFLVVIQFGSILAVLVIFFRKLNPFDSAKNIKQKKETVRLWLKVIIAVIPSG
                     VIGILFEDDIDRLFFNSTVVAIALIVYGIIMIGLEKRNKKPKYKDFSQVTYKLALCIG
                     LFQCLALIPGTSRSGSTIIGAVLLGTSRYVAAEFSFFLAIPTMLGASALKLLKAGFGF
                     TGFEWLILGVGSVVAFVVSIVVIKFFMDYIKKHDFKVFGYYRIVLGIVVLAYFFLL"
     gene            43521..44429
                     /locus_tag="ACGI59_000607"
     CDS             43521..44429
                     /locus_tag="ACGI59_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFG9738415.1"
                     /translation="MYSTKILDDYEYETFNVFSTKLNPNTKHDYLSKVILFKEFLKGK
                     ELIYATKEDCKNFVDYIQTKYAKSTCEKIYSYLHSFYNFLKKEGYIDINPFRYVEKPT
                     VTRIKTKDDVLSIQEINKLIGILPKLNIRDRVIIVCLVTTGCLLNELVSLKWKDLMVD
                     ENDNSYVRLGKGRKERVVKLHPYFFKLLEDYRNYSGLPEVIIPSDDFIFTTQKSNSIT
                     DRNVRLIVKKALDLAGLSQYSARDFRHSFAAISLRLGADESDVKNQLGWSDKYYAIRY
                     KYVLNFVDSEIVDYLIEKDNLDINKK"
     gene            44573..46018
                     /gene="purB"
                     /locus_tag="ACGI59_000608"
     CDS             44573..46018
                     /gene="purB"
                     /locus_tag="ACGI59_000608"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFG9738416.1"
                     /translation="MIDNKYTTYSNPLTDRYCSKDMSYIFSPQFKFSTWRRLWVALAE
                     GEHELGINITEEQLDELRANIDNINFDEARKIEKEVRHDVMSHVKAYGLQCEKAKGII
                     HLGATSAYVGDNTDVIQMNEALKLLRVKLVNLINNLKEFALKYKDMPALGFTHFQAAQ
                     LTTVGKRACLWAQDLILDLEDLNYRIDNMKLRGVKGTTGTQASFLSLFEGDHEKVKEL
                     DKKICKKMGYHSSYAVSGQTYTRKLDYQVISILSGIAQSMHKMTNDIRLLQSLKELEE
                     PFEKNQIGSSAMAYKRNPMRSERIASLSKYIISESISPAMVQATQWLERTLDDSANKR
                     LAIPQAFMAADAILEIGINVTDGLVVYENMINRRVNEELPFMATETILMEAVKRGGDR
                     QELHEIIREYSMKAAYRVKHEGKDNNLIELIMNDDSFKMSKEEILSIMDPKNFIGRAP
                     EQVVEFVNEVVEPAIKDYKEDLGKVDVDLRV"
     gene            46329..46781
                     /locus_tag="ACGI59_000609"
     CDS             46329..46781
                     /locus_tag="ACGI59_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFG9738417.1"
                     /translation="MNYIVKDTISFLVKTFPKIYSSLYLEDLKKFAPDYNVNKTQLRA
                     LVFIKNYGVISMTDLCSKLNIEKGSLTSMVDDLTDKKYVTRKRDLVDRRKYLIDITEE
                     GDKIATDFMDKLSDGLGEKLSKLTEEDRKKYLEAINTLQYILNKEEFR"
     gene            complement(47116..48312)
                     /locus_tag="ACGI59_000610"
     CDS             complement(47116..48312)
                     /locus_tag="ACGI59_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFG9738418.1"
                     /translation="MNYSNRVSAMQASPIRKLVPFAQAAKDKGIKVYHLNIGQPDIKT
                     PKGFFDAVKNFDSEVLEYATSEGIPELLEALQNYYKTYDMNFEKDELLVTNGGSEALL
                     FTMMAVCDPGDNLLVPEPFYTNYNGFGQSVNVEVNAVTTKAENGFHLPSKEEILSKVD
                     DKTKAIILSNPGNPTGAIYTKEELNILAEIAKEKDLWIIADEVYREFVYDGLEYTSCG
                     NLEGVQDRVIIIDSVSKRYSACGARIGSIACKNKGLIAQILKLCQGRLCVPTLEQIGA
                     VELYKTPVSYFKEVNEEYKKRRDVLYNELMKVEGVICKKPTGAFYIVAKLPVENAEDF
                     TIWMLKEFNKDNETVMVCPAEGFYATPGLGRDEIRLAYILNEKDLHRAATLLKEGLEQ
                     YVALQK"
     gene            48697..49647
                     /gene="galT"
                     /locus_tag="ACGI59_000611"
     CDS             48697..49647
                     /gene="galT"
                     /locus_tag="ACGI59_000611"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HFG9738419.1"
                     /translation="MKELRIDPITNDVVIFATDRLKRPLDTADIPNEDEETNEYDEEC
                     PFCKGNEAHATDALFEIDSEEGWLVKSVYNKFPIIDDMARDVYGVHEVMIESDKHNRS
                     FYNMSQKEFEDVFFMYRNRFRDLSKDDKVEYVSIFKNFLRKAGASLMHPHAQILSMSF
                     IPPEITNELLVSKEYYDSNKSSLYDDLIENEISLDRRVVYNGEAFLVLIPYATKYSGE
                     VRIIFKDKIEFGELNDNNIQELSTIFEKLFKKLYNINGYMPFNLCIHTHPTKIETKSY
                     FNVHMHIIPRKYNFGGFELGTNMYVSSMSPEDLTKKLKID"
     gene            49808..50560
                     /locus_tag="ACGI59_000612"
     CDS             49808..50560
                     /locus_tag="ACGI59_000612"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFG9738420.1"
                     /translation="MKFISWNVNGIRACVGKGFLDFFKEVDADIFCLQETKLQEGQIE
                     LDLPGYFQYWNYAERKGYSGTAIFTKKEPLKVMYGINIEEHDKEGRVITLEFEDFYFV
                     TVYTPNSQSELKRLEYRTRWEDDFIDYLTKLDNHKPVIVCGDMNVAHKEIDLKNPKNN
                     MKNAGFTKEEREKFSKLLDSGFIDTYRYFNPDKEGVYSWWSYRFNARKNNAGWRIDYF
                     CASKKLEDRLVSADIHTEILGSDHCPVELEIK"
     gene            complement(50652..51428)
                     /locus_tag="ACGI59_000613"
     CDS             complement(50652..51428)
                     /locus_tag="ACGI59_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HFG9738421.1"
                     /translation="MEYIIFDLEFNQGFDKKLNKTVSNEKCPFEIIQIGAIKLDSKFN
                     IIDTFNSYVKPTIYKDIHPFISRMTNIKSSDFNDSPTFPEVYNNFIKFISSQDPILCV
                     WGAGDLKELYRNINYHKLPSNSLPKSYINIQQHASKYFNNPAGKSIGLQNAISILELD
                     EKMSYHNALNDAYYTAKVFIKIYNPSIVPDIYLYTSIKPKTIRYSNKKRVDYDKLFDE
                     FRKILNRELTKDEKKIINLAYNMWKTNQFTLENVKQRKNK"
BASE COUNT        20662 a         5582 c         9179 g        16022 t
ORIGIN      
        1 tgataatata aacatggagc ttaatgaatt atttaatttt aagtatttaa taatagctgt
       61 aatttttatt ataagtgtaa ctatgttgat tttaatatct catataaaca tcttaataat
      121 agctgatata aaaaacaaag atatttgttt aaagcttaat ataaaataca tgtttaattt
      181 aataaatata aatagacaac tttatccagc tgaaaatagt aaaaataatg ataaaaaaga
      241 ggggatgaag aataatatag attcaagtat attattagct gatgaccttt tgagcattta
      301 tagattatta aaaaagataa aaatacatga actttattca aatattaatt ttggaactgg
      361 aaatattggc ttaacatctt ctgtatatgt attgataaat actttgtatg gaaatttatt
      421 taatatgatt gatgcagaaa aaatgtattt aaatgtaaat ccagatttca caaaagatta
      481 tgttttagga aatataagga tacatataag gccaagaata aaagctttat ttaatattat
      541 aataatgatt aataaaatta tgaataaaaa taaaggaaat aaggaaggtg atagtaatga
      601 gagcaacagg ttcgatacag agtcttatgg aaacaactct tgaaacaatt aaaggttcta
      661 tagatgcaaa tactattata ggagacccta taaaaacaga tactacagtg gttgtaccta
      721 tatcaaaggt tacaattggc tttggtatag gaggaggaga atactcaaaa ggatatgatg
      781 ataaagatag ggaaattgaa ttaaaaaatg aaaaatcaga tacgaatttt gctggaggta
      841 gcgcaggggc tatatctgta caaccagtag catttgtagt agttgaaagt ggagagacta
      901 gaataatgag tttagatagt aatatcaatc tagtggataa tatattatct ataactccta
      961 gagtattgga gaaaattcaa aatatatcac aaagtaataa gaatcaagat aaaaacaata
     1021 tgtaagaata aaatattttt aaatatgtat atagttttaa gtaaagtagt ggggttaata
     1081 taaatcccac tattttgttg tgtactttaa acatttatat ctaataagat tttagtttaa
     1141 aacaatttaa atagataata aagttgatat atagtataat tataaaaaga aaagaagtat
     1201 tatattatag atatataaac gcatttgaga tataagaggg agttataaat cattagtttt
     1261 gaggtgaatg ttatgagcga taaagaatta tctattttgt tgttagaaag tatgaataat
     1321 tttcataatt taataaagat tattaataac gaaagatata aaaaagataa ggttcttaca
     1381 gaaagacagt tctttgcctt agtaaaaata agaaaacatg ataaaataga gcttaaaaat
     1441 ctaagtagag atttgcatgt atctacttct agtctgtgta tactacttaa taaattggtt
     1501 gagcaagagt atgtatatag ggaagaagat agtagagata gacgaaatac tttttatgga
     1561 ataactaaaa atggagaaaa gatattggat aatgaaatac taaagtttgt atctataata
     1621 agtgataaaa tggattgcct agatataaat aataaagata aattatttac ttctttggaa
     1681 gagtctaaaa atataataga acaattattt tgaaaaggag aatttattat gaatataaag
     1741 tattctaaag aaatattaaa aagtttagaa aatgctataa cagtagtagc tctatttgag
     1801 ggagaagatg tttctacgac atttgataaa ataaatatga taagcagaaa ttttaattct
     1861 aaagaaaata gtatagatac tgtaacattg gttgaaaata acaatttggt aaatgtttgt
     1921 cttgttggtt ttggtaagag agagttattg aatagagaaa aaattagaat cataggtggg
     1981 aatttgtgta aaaaattaaa aaatatattg aaaaatgaag catataagag tagtgatgtt
     2041 aatgtactga atcttaattt aaatagtgat gaaataggtt gttttgtaga agggatatta
     2101 cttggaaatt ataagtttga taaatataag acaaaaagta aggattttga aagaaaagaa
     2161 gttgatacta taactatatt tactgaaaaa gaagttgagt gtcaaataga aaaggctaaa
     2221 atattagcta attcaactat aatagctaga aatctagtta atgaaccatc aaacgtaata
     2281 tatccaaaaa cattagcagt agaaacagtt aaacttggtg cagagtttgg gtttaatgtt
     2341 gatgtttatg aagaagaaaa aataaaggca ttgggaatgg atgctttttt cgctgtttca
     2401 agaggttcaa ttaataaacc aagattcata gtaatgagat actttggaga taaagaaagt
     2461 acagatatat taggtctagt aggtaaagga ttgacttatg atactggtgg atattcttta
     2521 aaatcaaatg cttctatgct agatatgaag acagatatgg ctggtgctgc gagtgttata
     2581 ggtgctatgt gtgctatatc tcaaagtaaa ttaaagaaaa atgttatagc agtagtggca
     2641 gcatgtgaga atgctttatc aggaggttca tacaaacctg gagatataat tagttctatg
     2701 gcaaagaaaa ctattgaagt tttaaatacg gatgcagaag gtagacttac attagctgat
     2761 gcaatatact acattataaa caatgaaaaa gtaacaaagg tggttgatgt agctacatta
     2821 actggtgctg ctttaatatt gttaggtaat gtcgcaactc ctatagtaac aaataatgat
     2881 gatttttatt gtgaattaga aaaagctgcc actttatcag gggaaagggt atggaagatg
     2941 ccaatttatg atgaatttaa agatatgata aaaggagaag aagcagacct taaaaacact
     3001 ggtggtaaaa atgctggatg tataacagca ggagctttta ttggtgaatt tgttgggaat
     3061 actccttgga tacatatgga tatagcgggg acaagtacat catcaaaatc aattggatac
     3121 aaagcaaagg gggctacagg agaacctgtt agaacattgt actatttggc agaaatctaa
     3181 aaaacaatta taaaatataa tttataaagc cacgtggata tttttgtaat ttaagattat
     3241 ctatgtggct ttatttttta tttaaataaa ttatttactc tttttctttt tttcaagtat
     3301 aaaagtatct ctattaaata aaagagcagg tatgtacata atactagtaa caaaaataaa
     3361 cgtaaaaata gcagttgtgc tatgacttaa aaaaccataa tagtacctag agttggtaaa
     3421 tgtgtttagt aaaaatatga atgaaaaata aataaaacat atagctaagt tatctctaag
     3481 tgcatatctg ttaactctaa attttctaac atcaaccata tatatatctg cataaattat
     3541 caagcactta gagaagataa agataaaata tattaagcta ggaaattcat agcccaagtt
     3601 tttagaaaaa atgagttctc caactgaaca aactaaactc caagaaaaaa atatattaaa
     3661 aagatgatac tgcttaaaaa ttagtatggc tatacacaga tatatacaaa tataattgat
     3721 tccaattggt aaagagttta gagtactata attacctgaa gaaataagta ataattgctc
     3781 tagagccata attattacaa gtaatgtaca aattatcttc tcaactgtat agctataagg
     3841 taaaatattt ttggttagtt ttggacaata gtataaaaat aatccaaata taaagagact
     3901 tacaaattgt tcactagaaa aaagaaaaat tttaaacata taacattctc tcctataata
     3961 aatttaatat gtatctatat aaatatattc attatatctc aatttaatat ataaaaaaga
     4021 taataatagt tagatttttt atatgaattt taataggtaa aatatcctat tgttttgtag
     4081 atatatccta attatgtcaa aaaataaaaa aatttttatt tattagagtt tttggaaata
     4141 ataataacag atattataat taattaacag tttttgtaag tatttgacaa tagagagggt
     4201 gtaacaaatg ggaataatag agtcagcatc aaaattggct gagatggttc atttattggc
     4261 tgttgaaaag gggataactg atatagaagc atgggatgaa gctgtgaaag aatacagcaa
     4321 aatttatgaa gaaagaagaa atgaatagaa tgataaaaaa tgaaaacttg atgataaaaa
     4381 ataactttat ttaaagaaat tcaattttta tagaagtatt aaaaataata taaataaaaa
     4441 gatggtattt caaatgtaag taaaaaatca ttttgggata ccatccttat tatttatttt
     4501 aataatatat aagtaataaa tcttacttat ccatattttt tatgtaacct cttttttcta
     4561 acttttttat atgctcctta cgtatttcat caagattaat accatattgt tcttctagct
     4621 taaacatcat agtaacacat gtctgagcta catccattag ttccttagca gttttcttta
     4681 taatatcaac ttcatctaaa tcattatttt ctccacttag atttctgaat ttacctatac
     4741 attcagctaa ttcaccttgt tcttcaccca ttttgataaa tgtagattct agtgttgggt
     4801 ctaaattatt aagctctggt aaagatatag tttttagttt cataaaagct cctttcaagt
     4861 tattcgtatt tatactaaaa catcataaca aaaataatac taaaaaaaaa ctttaaattc
     4921 aacataaaaa cttacatata taataaaaat cgtgaaataa atctatattt gtcttaatac
     4981 agcctcatat aaaacttctc caataggttt atttattaca agagaagctg acttatcata
     5041 gactgtattt gctttattaa taagagcaat ataatcacct ttgtaataat ctataaaact
     5101 tgctgctggg tacacagcta atgatgtacc tcctataatc aataaatcag cattacttat
     5161 agcagaaatt gttttagtaa tgacatcact gtcaagagct tcctcataaa gtactacatc
     5221 tggttttact atagaaccac agttgtcaca gtaaggaata ttacctccca gatttaacat
     5281 agattctaag tcaaaaaact taccacattt agtacaataa tttctatgta cagaaccatg
     5341 aagttctagc acatttttac ttccagccat ttgatgaagt ccatcaatat tttgagttat
     5401 gacagcttta agcttaccca tttcttcgag ttttgctaaa gctatatgag cattattggg
     5461 ttttgcatta ggatatatta atttatcctt ataaaattca aaaaattctt ctggatatct
     5521 aacaaaaaaa gtatgtgaaa ctaattgttc agcagtaaat tgtttattga gtttttggct
     5581 aaataatcca gtagaacttc taaagtctgg aatattagat tcagttgaaa cacctgctcc
     5641 accaaaaaat acaatattat tgtgattagc tattaagtct tttaagctat ttgcatccat
     5701 agttacacct ccaaattatt atatataaat tgtactacat attaggtcgt ttatgtttta
     5761 ttaatttaaa taaatatata gtttaataag ttaaataaag ggaatataaa taaaatgtat
     5821 acagagtata aatagacaat atttactaaa ataacaagtt gtcgtataat tatattaaaa
     5881 ttgttttagt gtataagaag ggcgggggaa gtacgtgaag aaaaaagcag ctttagcaac
     5941 attggctatg ttacccttag gtgtggtaaa tgcacatgct gatggagata taggtatagt
     6001 gactataaat tatttaaatg taagaaatga accaactgcc gaaagtagca tagcctttgt
     6061 tgctaaaaaa gatgataaag ttttgattaa agactcttct aatggatggt ataaaataaa
     6121 agctgaatct ggacaagaag gttgggcttc atcaaaatat atagcaaaat caaatagtga
     6181 ctctttaaga acgtctacaa ataaagaaaa acaagttatt tcaaatagtt taaatatgag
     6241 aaatggagca ggtacaagtt acagagtaat aactgtacta aaaaaaggtc aaaaagtaga
     6301 agtaatatca gagagtaatg gatggtctaa aatcaaatat gatggaagac taggatatgt
     6361 atctagctct tatttaggag acgtttcaaa ctcaactaat aaatcaaaaa ctaaacaagt
     6421 gaatacaact tcactaaatg taaggagtgg accaaataca agttatggtt tgttaggcaa
     6481 gttgccaaaa ggaagtaaag tagaagtaat atcagaaggt aatggatggt ccaaaataaa
     6541 gtataatgga aaagatgcct atgtatctag tatgtactta tcggatgtaa gtcaaagtaa
     6601 ttcagacaat tctagtcaaa gcaatgataa aaagaatact gataaggtcg taaatacagc
     6661 ttctttaaat gtaagaagtg gaccaggttc tacatatagt aagttgggaa aagtttataa
     6721 aggaagtaaa gtaactgtac tatcagaaag tagtggatgg gctaagatta attttaacaa
     6781 taaagaagca tttgtagtag gcaattattt atctacttca gcagatactt caaataataa
     6841 ctcaaatagt aactctgata acagttctaa tagtaatggc aataattctt catcatcagg
     6901 tcaagtaaat ggtatgtcag gtataagtgg ggctaaaatt gattataaat ctttgagtta
     6961 tactttagaa tctcatataa gtaaacaagt tgagaaagca gcatcaggag gaaatgtgat
     7021 agctccaagt aataggaaaa gcactccaag tcctgaattt agtacttttt cagctcaaag
     7081 aacaagctca tttgtaaatg caagttcaag tgatatagaa tattatttaa atcctaaaaa
     7141 ttttacaaat actactaaag gtatgatgca gtttttaaag attaacagtt atagagatgg
     7201 tatttcagaa tcaagtctta actcgtatct taatggttta tcttctagtg tatttaaaaa
     7261 ccagggagca gcctttataa atgctgccaa gaagtataat atagatgttg tatatctagt
     7321 atctcatgct atgtgggaaa ctgcttatgg taagtctaca cttgcccaag gtcaaacatt
     7381 aacatcttat aagggacaac ctcttagtaa gccagttaaa gtatacaatt tttttggtat
     7441 aggagcaata gataaaagtg ctaatgtttc aggtgcagaa gcagcgtatt ccaatggctg
     7501 gactagtgta gaagctacaa tagatggctc tgcaaagtgg atatcacaaa attacgtaaa
     7561 tagttctaag tacaatcaga atactatcta caagatgaag tggaattatg attatacatg
     7621 gcatcagtat gcaactgatg tgaactgggc taatggaatt tctggaatta tggaaaattt
     7681 aattggtctt tatggaggag gaagtagttt ggtttttgaa gttcctcaat acaagtaata
     7741 cataaataaa aaatatagac tatgggtagt tttcatttta tttaaattat ctatagtctt
     7801 tttgtatata atttataatc tgtaaattta atgctaacaa aacttttatg ttataatatt
     7861 aattctaagt aaatataagt gagtaatagg tagaaataga taaaaagtgg taataatata
     7921 tataaagata attttgagaa aggaggatat atatggaaag tattaaggaa tatttggata
     7981 agttagaaat taataatagt ggtcttggaa agcaacttaa agaagtttat ataaatagag
     8041 tagtttattt taaggaagat aagatagttt atttttatct aacttctaaa gatattgttt
     8101 ctcatgaact tttagataaa tttaaagaag aattgatgta taaactcgat tattttaaag
     8161 atatgaggat gaagataaga tttactggtc ttgaaagaaa atccaataaa gatgttataa
     8221 aaaaatattg gaataatata ttgtatatat taaagtatct ttgtccatct attgcagggt
     8281 ggtataaaca agtagaattt ctttgtttag aagaagaatt aaaaataaaa cttccaaaag
     8341 gtatatttta tgagcgatta atgaaaaaaa atgtaactta tgtacttaaa accgtgctta
     8401 gcgaagagtt gggattagat ttaaatataa caatagaaaa agctgttgat gaaaaggtaa
     8461 ataaagaaag actcataaga atcaatgata gagaaatgga agaaaaaatt agggctttag
     8521 aaattggaaa agttaataac tgtgaagaaa atgaagagga aagttatgtc attaagtctg
     8581 atgtagatga aaatttaatc tatggagata atgcaaatgc tatgattgaa aatatagttg
     8641 aattaaatgc atcttctgga acagttgcag ttgttggaga tatatttgat gttgacacta
     8701 aagaacttaa aaacggaaaa atacttatga tagcttcaat tacagattat actagctcaa
     8761 taagctgtaa acttttttta acagatacaa ataaggatgg agtacttgaa aatgttaaaa
     8821 aaggagcata cttaaaaatc aagggtgaca tagtttatga tacatatcaa agagaaatta
     8881 gtatgatgat tagtgggata agaaaagaaa ctagaataga gagagaagat aaatcagagg
     8941 aaaaaagagt agaactccat gcacatacac aaatgtcttc tatggatgct atttgttctg
     9001 ttaaaaagct tgtagagcgt gcagccaaat ggggtcattc tgctattgct ataacagacc
     9061 atggtgttgt acaaggattt ccagatgcta tgaatgcagg taagtcaaat aatataaaaa
     9121 tactttatgg agtagaaggg tacttggttg aagatgattc tctcattata gaggatgcaa
     9181 atgataaaga actttcgcag acatttgttg tatttgacat agagacaact ggtttttcta
     9241 atacaaatga taaaataact gagattggtg ctgtaaaaat agaaaacttt aagattgtag
     9301 atagatttag tgagttaata aatcctgaaa aagatatttc ttataagata caggaattga
     9361 caggaataac taatgaatta attaaagaca agccaaccat tgaagaagtt cttcctaagt
     9421 ttatggaatt tgttggagat agtgtattag tagcacataa tgcagagttt gatactggat
     9481 ttatatcgca aaaatgtagg gaacaaggct tgttatataa taacaaaaaa gtagatacac
     9541 ttatgttagc aagagtaatg ttgccaaact taaaaagaca tagattaaat gtcgttgcaa
     9601 aagaacttgg tattcctctt ttaaatcacc atagagcagt ggatgatgct gaagctacag
     9661 cacttatatt taataagttt ttgcagatgc ttacagaaaa aggtgcaaaa actctaagtg
     9721 atgtaaacaa tatacttgga aagattgatt acacaaagct tagcacaaat catataacct
     9781 tgatagcaaa aaattctata ggtataaaaa atttatataa aataatttca gatgcacatg
     9841 taaatcattt ttttagagca ccaagaatcc ttagaagtgt attaaatgaa tataaagaag
     9901 gattaataat tggttcggct tgtgaagctg gggtggtttt tcaagctgta aagaaaaacg
     9961 tgtctgatga ggaaatgaaa aaaataatag atttatacga ttatattgaa gtaatgccta
    10021 tagataataa tagatttatg attgataaag gagaagtaaa ggacgaagaa gagttaaggg
    10081 agttgaatag gaaattaatt gatacagcca aaaaatttga caaaattcca gtagctactg
    10141 gggatgttca ttttttagac aaacatgaag ctgtcttaag aaaagttctt aaatattctc
    10201 aaggttttaa agtagatgaa gaagaaactt atttacattt tagaacaaca gatgaaatgt
    10261 tggaagagtt tagttatctt ggagaagaat tggcatatga agttgtagtt gaaaatagta
    10321 atctgattgc cgatatggtt gaagatataa aacctatacc agatgatact tatcctccaa
    10381 ttatagaggg ctcagatgtg gagttaagag aaatgtgtta caaaaaagcc aaacgaatct
    10441 atggagaccc aataccagaa atagttcaaa aaagactgga tagagaatta aattcaataa
    10501 taagcaatgg gtatgcagtt atgtatatta tagctcaaaa actggttgca aaatctcttt
    10561 cagatggata tttagttggt tcaagaggct cagttggctc atcttttgca gctactatga
    10621 gtgatataac agaagttaat cctcttccag cacattatat ttgtgaaaac gaagactgta
    10681 aatattcatt cttttatgaa ataggtgaat ggggctcagg tgttgattta ccagacaagg
    10741 attgtccaaa gtgtggaaga aaacttgcta aaaacggtca tgatattcca tttgaggttt
    10801 tcttaggttt tgaaggtgat aaagaaccag atatagactt aaatttctca ggaaattatc
    10861 agccaacaat tcataaatac acagaagaac tatttggaga ggggtatgta tatagggctg
    10921 ggactatagg tactgtagca gaaaaaacag cttttggata cgcaagaaaa tatgtggaag
    10981 aaaataatat aagtgtacca aatgcagaag tacttaggct ttcaaatgga tgtactggag
    11041 taaaaagaac ctcaggacaa catcctggtg gagttatggt tataccagac tataaagatg
    11101 tatacgattt tacacctata cagtatccag caaatgatac ttcttgtggt gttataacta
    11161 ctcactttga ttatcattca ataagtggta gaatacttaa acttgatata cttggacacg
    11221 acggtcctac cattataagg atgcttgaag atatgactgg aataaatata acagaaattc
    11281 ctttagatga taaagagact atgtctctat ttacatctac agaagcatta ggtgttacac
    11341 cagaagaaat aaactgtcct ataggttgcc ttgctgtacc agagtttgga actaagtttg
    11401 tacgacaaat gttacttgac acaaaaccta ctacatttgc agaacttgta cgtatatctg
    11461 gtctctctca tgggacagat gtttgggtaa ataatgcaca agacttagtt agagaagata
    11521 tagttggatt aaaagatgtt atatctacac gagatgacat aatgaactat ttaatattta
    11581 aaggattacc tcccaaaatg agctttacaa taatggagag tgtaagaaaa ggtaaaggtt
    11641 taaaaccaga acacattgaa gaaatgaaaa aaaataatgt tccagaatgg tatataggct
    11701 catgtaaaaa gattaagtac atgttcccaa aagcacatgc tgttgcttat gtaatgacat
    11761 catttagaat tgcatattgt aaagttcact atccagaagc attttatgcg acttatttta
    11821 cgacaaaagt tgaggatttt gatgcagatt taattgttaa aggattagat gctataaaat
    11881 caaaaataaa tgaaattgag tcactgggaa atgatgcaac cacaaaagaa aaaggtatgt
    11941 tgaccgtttt agaagttgct cttgaaatgt atgctagagg aataaaatta ttaccagttg
    12001 atatatataa atcagatgca actgagttta tagttgtagg agaaaaaaca ttacttccac
    12061 caatggcagc aatacaaggt cttggaggaa atgcggctat aaacatccaa aatgaaagaa
    12121 aaaatggtga atttatatca aaagaagatt tgagaaagag aacaaaaata tcaaaaacgg
    12181 taatagaaac tcttactaat catggttcac ttgagaatat gagtgagaga aatcaattgt
    12241 cattgtttta agttagccca aaaagttgca aataaaagaa ttttatgata tcatagtata
    12301 gaaaatagta tactttgcag aaactaagag tgagacagct gttctcactc ttttatttta
    12361 aatatattat taaaaagctg gtttattttt attaaatata ttttatttaa gattatataa
    12421 actggtcatt tacaattgaa taggaggaac tttaatgaaa aaaaatttag aagctacaat
    12481 tgaggagatt gtaactaaaa taacggatga acatggattt gaaatggttg atgttgaata
    12541 tgtaaaagaa gcaggagaat actacttaag agtatacata gataaagaag aaggaatatc
    12601 tttaaatgaa tgtgaattag ttagtagaga attaagtcca atactagatg aaaaagaccc
    12661 tataaaagaa aattactttt tagaggtatc ttctccaggg cttgatagag cattaaaaaa
    12721 agacagagat tttgttagat atcaaggtag agatgtagat ttaaaattat ataaaccatt
    12781 aaatggatgt aagcaatttg aaggtgaact tgtaggatta acagaagata ataatataaa
    12841 aatcattgca aatggtaagg aaatggaatt taatagaaaa gatgttgcta ttgtcagatt
    12901 agcaataaag ttttagtggt atattaaata gggaggatat aaaaaatgaa tcatgaattt
    12961 atggaagcat tagatgaatt agtagttgac agaggtatag ataaagaaat acttatagat
    13021 actatagaac aagctctttt aactgcatac aagaaaaact ttggttcagc acaaaatgtt
    13081 agagttgaat ttgatagaga caagggagat ataaaagtat tttctcaaag agttgttgtt
    13141 gatgaatcag atttatatga tactttctta gaaatagagt tagcagatgc tagagagata
    13201 aaccctaatt atgaattagg tgatattata gaacatgaag ttacacctaa aaactttgga
    13261 agaattgcag ctcaaacagc aaagcaagta gttgttcaaa gaataaggga agctgagaga
    13321 gaaatagtgt ataatgaatt tatagaaaaa gaaaatgaaa tagttacagg agaggttgct
    13381 agagcaaata aaaatgttgt ttatgtaaat cttggaagaa tagaaggtat aatgactcaa
    13441 agtgaacaaa taccatttga gaattataaa gcaggtcaaa aaataaaatt ctatatactt
    13501 gaagttaaaa agactaacaa aggacctcaa atagttgtat caagaagtca tccgggtcta
    13561 gtaaagagat tgtttgaaga agaagttcct gaaatatatg aaggaatagt tcaaataaaa
    13621 tctgtagcaa gagaagcagg gtcaagaact aaaatggcag taagatctat agatgaaaaa
    13681 atagacccaa ttggagcttg tgttggacca aaaggactta gagtaaaaaa tatagtggat
    13741 gaacttggcg atgagaaaat agatataata aaatatagtg aagacccagc agagtttata
    13801 tcagcagcac ttagcccatc aaaggttgtt agagtagata tagatgaaga tgaaaagtct
    13861 gccttagtaa tcgttccaga ttatcagtta tcgcttgcta taggtaaaga aggtcaaaat
    13921 gcaagactag cagcaaaatt gactaactgg aaaatagata ttaagagcga atctcaagca
    13981 gaacaagaaa tgttaaatct tgaaaattcc aaagaaatag aagctgagga tgttgaagct
    14041 gaatttatcg accaagaaga gttagatact tcagaggata attttagtga agaagagtaa
    14101 ggagggatga ttttgaaaaa agtaaaaaaa gtgcctcaaa gaaaatgcat agcatgtcaa
    14161 gatagagact ctaaaaaaga gcttataaga atagttaaaa ataaagaagg aaaaatattt
    14221 ttagacccaa caggaagagc caatggaaga ggagcatatg tatgtaaaag ctctgaatgt
    14281 ctaaaaaaag ctataaagag taaagcatta aataaagctt ttaaaataga tgttcctgat
    14341 gaagtctatg ataatctact tatggagtta gaagaatatg aaaaataata aagaaaaagt
    14401 actttcgttc ttagggctcg caactcgtgc agggaaaata gtgtctggtg atgattcaac
    14461 tttgcttgac cttaaaaaag gaaaagtaaa tttgatttta atcgcagaag atgcatcaaa
    14521 taatactaaa aaacttttta aagataaatc aacttttaga aatattccat atctattttt
    14581 ttcaacaaaa gaggaaatag gatttgcaat aggaaaatct ccaagagcag ttgtaggaat
    14641 aaaagatgaa aatttttcta aaaagataat tgaactaata gaaatttaaa ataaaatagg
    14701 gggtgatctt gtgtcaaaaa caagagtata ccaaatagca gaagaattaa atatatcaaa
    14761 tgaagagctt ataaataagt tagctgaatt agatataaat gtaactgaca aagattcagt
    14821 attagaaggt gaagagttag aattagcact tgaaatgtta ggtgaagatt tatctcaaga
    14881 aaatggaaat gttatagaaa tagatggaaa gttgacagta caagtattag caacgaaatt
    14941 agataaatct ccatcagaaa taattatgaa gcttatgaaa atgggaacta tggcaacaat
    15001 aaatcaagag ataagttttg agattgctgc tttggctgcc aaagactatg gatttgaatt
    15061 aacagtagct gaaagtgatg atacagaagc tttagaaata gaagctttaa tggaaattga
    15121 agaagataaa gaagaggatt taaaacctag accaccagtt gttacagtta tgggacatgt
    15181 tgaccatggt aagacatctt tactagatgc aataagaaaa actgatgtga tttcaggtga
    15241 agctggagga ataactcagc atataggtgc ttcagaagtt aaaatcaatg gacacaaaat
    15301 agtattttta gatactccag gacatgaggc tttcacatca atgagagcaa gaggagcaca
    15361 agttacagat atagcaatat tagtagttgc agcagatgat ggtataatgc ctcaaacagt
    15421 tgaagctata aaccatgcta aagcagcagg tgtaccttta atagttgcta ttaataaaat
    15481 agataaacca ggtgcaaacc cagataaggt taaacaggaa ttagcagacc aaggattatt
    15541 agttgaagac tggggtggag aagtaatagc agttccagta tcagctaaga aaaaagaagg
    15601 aatagataca cttttagaaa tggtattatt ggttgctgaa atggaagagt taagagctaa
    15661 tccaaataaa agagcagtag gaactgttat agaagctgag cttgataaag gaagagggcc
    15721 agttgcaaca gtacttgttc aaggtggtac tttaacagta ggtgacccaa tcgttgcagg
    15781 tgttgcatgt ggtaaagtta gagcaatgat aaatgcaaaa ggtaagagag taaaaacagc
    15841 aggaccatct acagcagtcg agatattagg tctatcagaa gttccacaag gaggagacca
    15901 atttgtcgaa gttccaactg ataagattgc tagaagtgta gcagcaagaa gacaacaaat
    15961 agttagagat gaaatgttaa aatcaactca aagattatca ctagatgctc tatttagtca
    16021 aatgagtgaa ggttctataa aagaccttaa tatagttata aaagcagatg tacaaggttc
    16081 agtacaggca gttaaacaat ctttagaaaa attatcaaat gaagaagttc aagttaaagt
    16141 aatacatggt ggtgttggag ctgtaacaga atcagacata ttacttgcag cagcttctaa
    16201 cgcaataata ataggattca atgtaagacc agttccaggt gcagaatctc taggagaaaa
    16261 agaaaatgtt gatataagaa catatactat aatatacaag gcaatagaag atatacaagc
    16321 ggctatgact ggtatgttag accctgaata tgttgatgaa gaaacaggaa aagctgaaat
    16381 aagagaaata tataaaatat ctggtgttgg aacagtagct ggatgttatg tgactaatgg
    16441 taagatattt agaaattgta aagtaagatt agttagagat agcataataa tacatgaagg
    16501 tgagcttgca gcacttaaga gatttaaaga tgatgtaaaa gaagttaatt ctggatacga
    16561 atgtggtatg agttttgtaa attataacga cataaaagaa ggcgatatag tagaagctta
    16621 tataactaaa gaagtagaaa gaaaattata aattcgtatc aaagaaaatc agttagtaaa
    16681 gaaggtcgat ggtatggcat catataatag aacaagaagg atagcagaag aaataagaaa
    16741 agtcgtaagc actatgctta taaatggagt aaaagaccct agaataacat ctatggtgag
    16801 tgtaactgat gtagaagtta caaatgacct aagatatgct tatgtatatg taagcattct
    16861 aggtggagat gaagaatcca cactaacagg tcttaaaagt gctggaggat atataagaag
    16921 agaagtagga aaaaatataa aacttagata tattcctgaa atagtattta aacttgatga
    16981 ttcaatagaa aaaggaatgt atatggatag ccttataaaa agagttaatg aaaaaaatgc
    17041 tcaacaaaat gaggatgaaa attatgatga ataatatgga tagtattata aactatattt
    17101 atgagggtga ctattttata gttacctctc atatcagtcc agatggagac aatattggtt
    17161 ctacactttc aatgtattat acattgaaga atttaggaaa aaatgtatat tatgtacttg
    17221 atgatgaagc tccattaaat ttaaggtttt tggttgaaga tgtaaaaatc tataagtcta
    17281 atgaatttaa tatgaagaac tattctctaa ttgcacttga ttgtggggat aagatgagaa
    17341 tatgtgtgag tgatgaaatc aaaaataatg catcaaaatt aatctgtatt gaccatcatg
    17401 ctagtaatga ttcatatgga gatttaaatt atgtagatat tgatgcatct tctacatgtg
    17461 aattagttta taatttatta gttagatttc agcaaataaa agatattaat attataaatg
    17521 aagatatagc aacatgttta tatacaggtc ttgtaactga tactggaaac ttttcgtatt
    17581 caaatgtaca tgcaagtagc tttgaaatgg caaaaaattt acttgtttta ggtgcacaaa
    17641 aaaatactat aatacaaaat atatatcaaa gcaattcttc tggttattac aaattattag
    17701 gagaagctct aaagggtttg gagatatttg attcaaaggt atctagtata gttttaactc
    17761 aagacatgat gaatagaaat aatattagct ttaatgatgt tgatggaata acaacatata
    17821 caagagacat aaaaggtata gaagttggaa ttttatttaa agagaaaaaa caaaatgaaa
    17881 ttaaagtgag ttttaggtca aaaaattatg ttgatgtaag tgaaatagcc aagttatttg
    17941 gtggtggtgg tcatgtaagg gctgctggat gtactattag agatagtatt gataatgcaa
    18001 agaaaatggt actagaggca gtgttaaaat caatttaagg tgacagtaaa atgaataaga
    18061 ttataagtat attgaaaccc acaggtatga catctcatga tgttgtaagt agagtgagaa
    18121 agattttaaa tataaaaaaa gttggtcata caggtactct tgacccagat gcttcaggtg
    18181 ttttaccaat ctgtataggg aaagctacaa aagtatgtga agttatttta aacaaagata
    18241 aatcgtatat atgtgagctt actttaggta taagtacaga tacttatgat gcttcaggtg
    18301 aaattttaaa aaaagttgat gattttaaat ttagtaatga agacatagaa agagcttttg
    18361 acacacaaag aggagagata aatcaacttc ctccaattta ttcagcttta aaggtaaatg
    18421 gaaagagaat gtgtgattta gtaagaagtg gtagacaatc tgaaattact ttaaaaacaa
    18481 gaagagtaaa tataaaagac ataaaaatac taagtataaa aggtaataaa gtaatgtttt
    18541 atgttgagtg ttctaaaggg acttatgtga gaagtatttg ccatgatata ggagaatatc
    18601 taggttgtgg agctcatatg tcgtttttga atagaacatc ttctggtaaa tttgacttag
    18661 ataattcaat aacattagaa gaattagaat tattttatga aaataaaact ttagacaaat
    18721 acttatatga tatagattat gtccttgatt cgtttaatta tgttgtatta aaccctaatg
    18781 ctataaaata ttatagtaat ggtggctcaa tagatgataa aaggttttta aagaataatt
    18841 tcgataaaga tgatgaattt gtaagagtat acagtacaga taatttctta ggattaggaa
    18901 agttatcaaa acacaataat acaataagtg ttaaaagtga taagatgttc atataaccaa
    18961 tgtatttaaa aaaacaacaa aagagatagt actataataa cttctatagt ctatctcttt
    19021 tacttatgct ataatagttt gtgaataagt taagagtaaa ttatatttta gcggggaaca
    19081 tttattatgg tgattataaa atctataaaa gaaatagcaa acataaaaga aagtgtcatt
    19141 accataggaa attttgatgg tgtacacaaa ggacatcaag ttcttatagg aaaaacagtt
    19201 gaacatgcaa aaaaagaaaa tatacagagt attgtattta catttgcaaa tcatcctgta
    19261 aattacttta gaccaaactc aacaaaaaat attataagca atgatgataa aataaaatta
    19321 ttagaagatt tgggaataga tatagttgta atcataccat ttgatgagta tatgactaaa
    19381 atacctgcaa aggatttcgt agatgagata ctagtaaaaa aattaaaagc taaaaagata
    19441 gtaattggac atgactttac atttgcaagg gcaaaagaag gcaatgttaa tctattaaaa
    19501 agcttagaac atgaatttgg ttttgaggta gaagtaataa aacccatcaa aatcaatgat
    19561 gtaagagtga gtagtactta tattagaagt ttagtatccc agggtgatat ggctaatgtc
    19621 aaggaatatt tgggtagaaa ttacaagtta gaaggttgtg ttatacattc taaacacctt
    19681 ggaagaacta taggattccc aacggcaaat attgacctta agaataatat gctcgttcca
    19741 aagagaggta tttatgcttc tattgtacat attggagatg agacttattt tggtgctaca
    19801 aatgtaggct ataacccaac agttaatgga aaaactttgt ctattgaaac aaatatttta
    19861 gagtttgata gagatattta tggggaaaat ataattgttg aatttttgga aagaataaga
    19921 gatgaaagaa agtttaattc aatagatgac ttaaaaaatc aattgtataa tgatacaaat
    19981 tatgtgtatg aaaactatgt ttgcaaaaac aagtaatgta tgataaaatg attaaggaaa
    20041 ataaccatta ctcggcattt gaatactcca atcaatgctt agtaataggc gtttctaaaa
    20101 aaatacggag gtaatctaaa tgataaacaa atcagaaata ataaaagaat atggaagagc
    20161 tgaaggggat actggttctc cagaagtaca aatagcttta ttaacagcta gaataaacga
    20221 gttaaatgga catttaaaaa ctcataagaa agaccaccat tcaagaagag gactattaaa
    20281 aatggttggt agaagaagaa acttattagc ttacttaaag gaaaaagatc ttgaaggata
    20341 tagagcatta atagctaaat taggattaag aaagtaattt cattctttat aaaaaagcag
    20401 gtatagtgta tacctgcttt tttatagtta attcattata aagtaaaata gttaataata
    20461 aaagtagttt atatgttata aaaaattata aaatgaataa tattgggtaa gaatattaaa
    20521 atagtataca aatacataat caatttaaaa ggaaagacag gataaatata gaaattttat
    20581 aacaggataa ttagttggaa taaggaggat attattttga aaataaagtt agtatgcgat
    20641 agtttgtgcg atattccaga tgaaatatcg gaaaaagatt atatagagat agttccactt
    20701 acagttattt ttaatgatag ggaatatata gaaggagtag atataaagaa agaagagttt
    20761 tataaaaaag taaaagaaat caagcaaata cctaaaactt cacaagctac atatatggag
    20821 tttaaagaag tgtttgataa gttcataaca gaaggttatc atataatctg tatgacaggg
    20881 gctgcaaacg catcaggaac ttttcaaagt gctatgatag ctaaaaatga tgtaaatgaa
    20941 aatgaaaaaa tacatatatt tgatactaga aatttatctc taggtagtgg acaatatgtt
    21001 ataaaggcat gtgaacttct tgaagaagga ctgggatttg aagaaattat agatgaatta
    21061 gaaaatactc gcagtagtgt aagattgctt tttgcaccat atacacttga ttttttaaag
    21121 cagagtggaa gagttccagt tgcaactgct ttgataggaa atatgttaaa tataaagcct
    21181 atatttttct ttgataatgg ggaagctaag ttggtaaata aggttagagg aataaagaat
    21241 atagcaagta aattggtcga tataatttta gaaatgaatg aagggtcttt agaaggaaaa
    21301 atcgtcacaa taggttgtgg agataattta cacgattgtg aaatattaaa agatgaagta
    21361 aataaaagaa taaaagcaag aagagtatta tttactcgag gaggagtttc tatttgctct
    21421 catacaggac cagatatatt ggctataagt tgttctaatt agtagtaata taaggttttt
    21481 gcatatatat gtgaagataa ttttatattg ttaatttata gtatttttga taatatagta
    21541 aatagagtga tatacgtgtt ttttaataag aggaggacaa gcattaatgt ttgaacataa
    21601 aatatttaaa atggattttg caggaagaga actttctgta gaaataggaa agatttgtga
    21661 aatggctagt ggtagttgta tagttcgata cagtgatagt atggtaatgg taaatacaac
    21721 taaatcagcc aaacctagag acggaataga cttttttcct ttaagtgtgg attatgaaga
    21781 aaaattatac tctgtaggta agatacctgg aggtttttta aagagagaag gcaagccttc
    21841 agagaaagct atacttacat caagattgat agatagacca ataagacctt tattcccaaa
    21901 aggatttaga aatgatgttc aggtagttgc aacagttcta tcagtagacc aagattgtac
    21961 accagatata gtagcaatga taggttcttc aatagccctt tctatatcag acataccatt
    22021 taatggacca acaggttcag tatgtgttgg ccttgtagat ggtgcatttg ttgtaaatcc
    22081 aaacgcagag caaagagaaa aaagttcaat gcatctagtt gtttcaggta caaaggaagc
    22141 aataatgatg gttgaagccg gagctgatga agttccagat gaggtaatgc ttgatgctat
    22201 tttatttgct caccaagaaa taaaaaaaat agttgagttt atagaaggta tagttgcaga
    22261 agttggaaaa gaaaaaatgc ctgttgaatt ataccatgca ggagaagaaa taactcaatt
    22321 ggttcgcgaa tttgcaactg ataagatgaa aaaagcagtt cagacttttg aaaagttaga
    22381 gagaatggaa aatatggata gagttaaaga agaaacttta gctcactttg aagaaacttt
    22441 agaagatttt gaagattttg ttggagatat agaagaagta ttacaagata taataaaaga
    22501 agaagtaaga aaacttatag tacatgaaaa tgtaagacca gataatagaa aacttgaaga
    22561 aataagacct atatggtgtg aaactggcat gatacctaga gctcatggtt cagccatatt
    22621 tactagagga caaactcaag tacttaatgt tgctacttta ggtgctttag gagatgttca
    22681 aaaactagat gggttagatg aagaagaaaa caagagatat atgcatcatt ataatttccc
    22741 tgcatatagt gttggagaag ctagaccatc aagaggtcca ggaagaagag aaataggaca
    22801 tggtgcttta gcagaaagag ctttattacc agtaatacca tctcaagaag aatttcctta
    22861 tgctattaga ttagtttctg aagtattaag ttcaaatggt tctacatctc aagcaagtgt
    22921 atgtggttca acattatctt tacttgatgc aggtgtacct ataaaagata tggttgcagg
    22981 tatagcaatg ggacttataa agcatgatgg taaggttgca gtgctttcag atatacaagg
    23041 tatggaagac catttaggcg atatggactt taaagtagct ggaacagagt atggtataac
    23101 agcaatacaa atggatataa aaattgatgg tatagataaa gaaattctac aacgtgcttt
    23161 aaaacaagct aaagagggta gaatccatat actaggagag atgagaaaaa ctatatctca
    23221 gccaaaacca gaactttcac catatgcacc taagattgtt aaaatgcaaa taaatcctga
    23281 caagataaaa gatgtaatag gacctggtgg aaagataata actaagataa tagacgaaac
    23341 tggagttaag atagatattg aacaaactgg ggaagtattt atatcaggta ttgagattga
    23401 tatgattaag aaagctcaag aacttataaa taatatagta gttgaaccag aagtaggaaa
    23461 aacttataaa ggtaaggtaa gtagaataat gaactttgga gcatttgttg agatacttcc
    23521 aggaaaagaa ggattacttc atatatctca tatagctcat gaaagagttg ccaaagttga
    23581 agatgtattg aatatagggg atgaagttga agttaaagtt acagagattg atgaaaaagg
    23641 aagagttaat ttatcaagaa aagttctttt accaaaacca gaacataaaa ataaataact
    23701 tattatataa ttaaaagcaa agtcttagga ttttgctttt ttaaatacaa aaaagtagat
    23761 aattttaaaa tgatagttat ggatggctta tagttctttt tctgtcagtc tttactctta
    23821 taaagaagct atttaaaagc tgattaatag aaagctttat tgatatatat agaatcactt
    23881 aagttatatt tagtgcataa acagagctta tttttaatag tattaaataa gaaacaattt
    23941 gtacataatt agggggagtt ttaatgataa tgatggtact taataagagt aagctcaaaa
    24001 aaatcataat actaatttta gtagtgttat tagtattatc aggaggggta atggtattaa
    24061 agttatttaa caacaagcca gtatttaatt tatttaatgg tgtagattta ccatatgaaa
    24121 gaggtacaaa ggataaaagt ggatatgttg cattaacctg taatatagac cttggatggg
    24181 aaacagagta cgtagaatcc attttagaaa cacttaaaaa agaaaatgta aagattacat
    24241 ttaatgtaac tggaaaatgg gcagaaaaga ataaggatga attattaaag ataaaaaaac
    24301 aaggacatga aataggtaat catggatata aacatttaga ttattctaca ttatcatatg
    24361 aagacaatta tgagcaaata gagacctcta aaaagataat tgaagaaatt ataggtgaaa
    24421 aaactaaatt ttttcaagca ccagcaggtt cctttggtcc agaaacagta aaggctgcta
    24481 aagcattagg gtatacttct atcaaatggg atgccgatac tatagattgg aaatacaaag
    24541 accaaccaga ggtaatcatt gatagaatga agaaaaaaga cattaaagat agtagtataa
    24601 tcttaatgca tccaaccaac gcaacgacaa aatgtataga tgatattata gctattgtaa
    24661 gagaaaaagg actaaaagca ggaaaactta gtgatgtatt taagtagaca aagtataaat
    24721 atttggttaa gattgtataa gttgtagttg aattaattaa aagactgact tataatgtaa
    24781 tacatataaa aaataaatat aattaaaaca tagtgttttc aataatccaa tatgaaacaa
    24841 agaataaatt gttaggagaa tgtaatgtat aagacaaaaa tactggaaaa tgggcttaca
    24901 ataattggtg aagaaatacc atatctaaag tcaataacat taggtatttg gataaatgca
    24961 ggctctagga tagaagaagc acaggtgagt ggaacttctc attttataga gcatatgatg
    25021 tttaaaggaa ccaaaaacag aacatcaaaa gagattgcta gttcaataga taatttgggt
    25081 ggtcaaataa atgcatttac aagtaaagaa tgtacatgtt attatgtaaa actaatagat
    25141 gagcatatag atacaggaat tgatgtattg agtgatatga tactaaattc taaatttgat
    25201 aaaaatgata tagataagga aagacttata atattggaag aacttaagat gtatgaagac
    25261 tctccagatg acttatctta tgatttatta gtggaaaata tatatgctaa tgatggatta
    25321 ggaatgaata taattgggac aaaagaatct ctatacaata taactagaga atccatgtta
    25381 gaatacttaa ataagtatta tattccaaat aacgctgtta tttctatagc tggaaacttt
    25441 aactttgatg atatggtgga gaaaatcaaa tcaaaatttg gacactggga aaagaaaaat
    25501 ttgagtatag acataagtga ggctaagttt aatccttgct ttatatcaaa aaataaagat
    25561 actgaacagg tgaacttagc tatgtgccta aaaggtattc cttttgaaaa tgatgaagaa
    25621 gtctattcaa tggcagtggt aaataatatt ttcggtggaa gtataagctc aaggcttttt
    25681 cagaaaataa gggaagaaaa aggtttagta tattcaatat actcatcaca gacattatat
    25741 aggaaatgtg gtgaattagg tatatttgca agcatgagta cagaaaattt acaagatgta
    25801 tataatttaa taaagaaaga aattgaaaat ataagagaaa actatcttac agaaaaagaa
    25861 atatctgaaa gcaaggaaca gttaaaagga aattatatat tggatttaga aagtactagc
    25921 agtagaatga tgtctactgg taaatctatg ttacttagta aaaaagtaaa aactacagat
    25981 gaaatacttg aatgtataaa tagtgttaat attaattcaa taaagaaggt tgttgataag
    26041 gtattcaata tcgaaaatat tggtacatgt atagttggaa gagatgtaga aaagattcta
    26101 cacttggatt aatattgaaa aaagtaaatc tctggaggta taaaatatga atttatctga
    26161 aatagcaggt aaggaaatag ttaacttggt cacaggagaa agattggggg tcattggaga
    26221 atgtgacctt atactagatg aaactacagg cggtatatta gcgcttctaa ttccaaaaga
    26281 aaaaggattt tttgccagaa gaaaagataa gtcattttta gaagtaccat ggagaaatgt
    26341 aaaaaaaata ggtaatgaca tgataattat agaatatgag ggatatattt aggaggaaaa
    26401 aatgagtatt ttagtgcaaa agtttggtgg gacatcagta gaatcatatg aaaaaatgaa
    26461 tgaagtatgt aagatagtaa aagcatacaa aaaaaatgat gaagaattac aattagtatt
    26521 agtagtttca gcaatgggaa gaaaaggagc tccatatgct acagatacac ttataaattt
    26581 gtgtagtgca gtaaatgatg agccaagtaa aagagaactt gatttaataa tgtcttgtgg
    26641 agaaataata tcaggaacaa ttcttgctaa tttattgaat gcacagggga tagattctgt
    26701 tttctttact ggtcagcaag caggtataat tacaagtgat gaatattcta atgcaaagat
    26761 aaagtatata aatcctaaaa aggtaaaaag agctttagat gatggtaagg ttgtagttat
    26821 agctgggttc cagggtgtaa cagatgatgg agaaataact actcttggaa gaggcggaag
    26881 cgatacatca gcagttgcta ttggtaaagc tttagaatgt gaaacagttg aaatttacac
    26941 tgatgtggat ggtataatga ctgcagaccc tagagtagaa ccaaacgcta aagtattgag
    27001 ttttatagac tatgaggaag tattccaaat ggctgacaaa ggtgcaaaag taatacatcc
    27061 aagagcagta cagttggcta aaagtggaaa tataacttta gccataaaga atacaatgaa
    27121 tccaactttt gaaggtacaa aaataggttc tttatgtaga cacttagaag acaatataga
    27181 atatgaacaa gaaagagatt ttaaggttgc tgttgcaaat aaagatagcg tagcacaagt
    27241 taagattaag tcagcagaag aagtgtttac agaagtatta aatgagatag aaaagaaact
    27301 tataactata gatatgataa atttctttat atcagaaaaa gcatttgttg tagaagatgc
    27361 tgatataaaa tcattaaagg aaatactaga taaatttgaa ttagattatg aagtaaatag
    27421 agactgtgct aaggtaacat tgatatgttc aagaattgct gatgaaatgt ctggtatcat
    27481 gtcaaaggta gttagaggat tatcaaaagc aggggtatcc cttttacaaa catcagactc
    27541 aaatatgact atatcttgtt tagttagtga agaagatatg catacagcag tacatgctat
    27601 acatcaacaa ttttatttaa aataaactgt ataatacaat tattttttaa tcaaatttaa
    27661 gaatttcata tatatcttaa tatatttgat ttatttggta aaactaaatt aaatataatt
    27721 ttgataggag tttttttatg aaagattata gagaagataa tgaaagattt gataaagata
    27781 attgtagtga agaatctagt tgcgaagaga gagaatgtat aaagcaattt ggtaccaatg
    27841 aaatgccacc tcaacctcct aaagatattc aatgtataac tataattgga gaaattgagg
    27901 gacattttat aggaaatcct caaaaaaaag ctacaaagta cgagcatata attccaatgc
    27961 tatactcaat tgaggaaagt aatgatgtta aaggagtgtt agttgtttta aacactgttg
    28021 gaggagatat tgaagcaggt ttagctattg cagaactttt aaacagtaca tctaaaaaag
    28081 tagttactct tgttctagga ggaagtcata gtataggtgt tcctcttgct acagcaggag
    28141 attactcatt tatagcacca acagcaacca tgattataca tccagtgcga actactggtc
    28201 ttgtgattgg gataaatgag acttttgagt attttaaaaa gatgcaagat agaataattc
    28261 aatttataat aagaacatca aatatcaaaa aagatgtact tgaaaaactt atgcatgaaa
    28321 aagatgaact tgtaagtgat gttggtagtg tattaatagg aaaagaagct gtggattatg
    28381 gtcttataga tgaagttgga ggactaaaag aagcacttaa aaaattaaga gagcttataa
    28441 aagaatctga ggaagaaaaa aataatgatt aaaatttgtt taaataataa aatttatgat
    28501 ataatgatgt aataaagtag gagtgttagt gttaaggcat tagcactttt tgctgtgaag
    28561 gggtaaaact tgctaaaaat aaggagtgat attaatggcc acgaaaaaga agaaaaaaaa
    28621 gaaggtaaat accttaagtt ttaatgcaga atatcataat ttaataacaa tatttatagg
    28681 tgtattttta ttatatagtt taaattctaa ttctatggga tggatacctg tgttaatgca
    28741 aaatcttttt aagggactat ttggagggct ttcaatagca ataccattta tagtaattat
    28801 aacaggactt ttaggatttt ttgatggaaa tgaatatata tataggctaa gaaagactaa
    28861 actatactac ataattattt tatttatatt tgtattttat ggattattaa atgcaggaac
    28921 tcttcctgta gatagcccac taaaaggtaa catgtttgat gatgttatga aattgggtgt
    28981 aagtggacaa ggtagtggtt tgatagcaac aactattgcg tattatatga gtaaaatatt
    29041 tggtatagca ggtggatggt taataagtat cttcgcactt atactttctg taatgtttat
    29101 atttaatatt tctataaaag atttggtgtc aaatgcaaaa tcaaaatcta aagcatctaa
    29161 agacagcaat ttaacgttta aagataaaat tgcaaatatg aaaaaatctg caatagatat
    29221 gatgactgat gaagtagatg atactactat aaataaaaaa ccaggtttct ttaaagggtt
    29281 aatgtcaaaa ggtcttaata atgatgatga agaagatgaa tattttgaag aagaaaatag
    29341 tgatggtgta gatgataaaa ctataaaaat agttggattt aataaggcag aagatgagta
    29401 tttagaaata ttagaaggaa ctcaaagtat gccagagtta gatgttttaa aagaattaca
    29461 aaaagctacc aatgaaaatc cagttgtaga tactaaacca gaaaaaaaag ttgatatagc
    29521 taaacctaat ttaaatatag aaaaaactca acctatgagt attgtagcag agccagttaa
    29581 tgaggattat tcaaactata aaaagcctag cattgagctt ttaaataaag taaataagaa
    29641 gtctgatgaa aatggtaaaa agaaagtgtt gaaaaatgct tctcttttag aaaagacact
    29701 atcagatttt ggagtggaag caaaaattaa tcaagttaca gttggaccta ctataacaag
    29761 atatgaaata caaccaagtc caggagtcaa ggtaagtaaa attgtaaatt taactgatga
    29821 tatagcttta agtttagcag ctaaaagtat aagaattgaa gccccaattc ctggtaaaag
    29881 tgcaattgga atagaagttc caaatgaaga agctcaaatg gttggtgtga gagaagttct
    29941 agaaagtgaa gaatttaata attttaactc tcctcttgca atgggacttg gaaaagacgt
    30001 agcaggaaaa attataattg gagatatagg taagatgcca cacttactaa tagctggttc
    30061 tacaggttca ggaaagagtg tgtgtgtaaa tacattaata agtagtattt tatataaggc
    30121 aaatcctgat gaagtaaaat tactattaat agacccaaaa gtagttgaac ttgcaaatta
    30181 taatggaata ccacatctat tgataccagt agttactgac cctaaaaaag ctgcaaatgc
    30241 tttaaattgg gcagttacag agatgaatag aaggtacaaa ttatttgcag atgctcaagt
    30301 aaaagatgta acaagttata atgagaaagc ggaagaaaaa cttcctaaaa tagtaataat
    30361 aatagatgag ttagcagatt taatgatggc aagtgcaaat gatgtagaag attatatttg
    30421 tagattagct caaatggcaa gagctgctgg aatgcacttg attgtagcaa ctcaaagacc
    30481 atctgttgac gttataacag gggtaataaa ggctaatata ccatctagaa tagcatttgc
    30541 agtatcatct caaactgatt caagaacaat ccttgatatg ggtggtgcag agaagcttct
    30601 aggtaaaggt gatatgttat tctatccact tggtgcagca aaaccagtta gacttcaagg
    30661 ggcattcata tcagagagtg agtcggaaaa agtaatcgat tttgtaaaaa gtcaagttaa
    30721 agatggaata aaatatgaag aagatataat tgaaactata tctaaggtta atacttcaaa
    30781 gggtagtgat gaggatgagt ttttgagtga agcgatagaa tttgtcgtgg aaagtggtca
    30841 agcatctgcc tctatgctac agagaagatt taaaatagga ttcaatagag cagctagact
    30901 tatagattct atggaagaaa gaggaataat tggagcaagt gagggaagta aaccgagaaa
    30961 ggtattaata agcaaacagg atttgcagaa tttagaaggt gaatagaatt gtcagttata
    31021 aaaatagaaa aagcacttaa gctagataaa gtaatatttg tagatgtgag aactgaagga
    31081 gaatatgaag aagaccatat attgaatgca atcaatatgc ctctttttaa aaataatgaa
    31141 cataatgacg ttggaacaat ttataagatg cagggaaaac atgaagcaat acaaaaaggg
    31201 tttgactatg tatcgtataa attaaaggat atatatttac aagcagcaga acttgcatta
    31261 aattatgata atattgtcat atattgtgca agaggcggaa tgagaagtgg cagtatagta
    31321 aatttattat catcattagg tgtaaatgta tatcaacttg aaggtggata taaggcttat
    31381 agaaattttg tattagagta tctaaggcat gtaatggata caaaaaactt tgtatctgtt
    31441 catggattga ctggtacagg aaagactgat ttgttacatt tattagatga aaaaggaata
    31501 gatgtactgg atttagaagg catagctaaa aacagtggtt ctacctttgg ttttataact
    31561 tttgataaaa agccaccttc tcaaaagaac tttgaaacaa aactatttga aagtatattt
    31621 ttttcgaaag agaattatat atttgtcgag agtgaaagca aacgagttgg aagtgttaca
    31681 gttccaaatg aaattttcga ggcaatgaca cgagaaggac atcatatatt attagaatgt
    31741 gatattgaaa atagagttga tagactttgt agagattata tttatgataa agatgaaggt
    31801 aatatattga ttttaaaaga atgtataaac aagtttagaa aaagattggg taataaagta
    31861 gtagatgatt atattgattt attagaatca ggtgaatata aagaacttgt aaaaagatat
    31921 cttttagaat actacgaccc actttatatg cattcagtag aaaaatataa gtatagtaag
    31981 attataaatt ttaatgatac acaaaaagct ttagaagatg ttatagactt atatgagagt
    32041 atattggaag gagaaagtga atgttaaaaa tagcattaga atcattagga tgctcaaaaa
    32101 atttagtaga tgcagaaata atgatgggaa ttttaaataa caaaggctat aaattaattg
    32161 gagattttga agaagctgat gtaattatag taaatacatg tggatttata gagtcagcta
    32221 aacaagaatc tatagatacg ataataaact ttgcagagtt aaaaaaaact ggcaatttaa
    32281 agttgttgat agtaacaggg tgtctagccc aaagatattc tgaagaatta aaaactgaaa
    32341 ttcctgaaat agatgctata gtaggaacag gaagctatca aaatatagat aaaatactta
    32401 aagaattaag tgaaatgcat aaaattgtaa gccttaatga tatagagttt gtatttaatg
    32461 aagatttacc aagatatata tctacaccta gctatatggc ctatttaaaa attggagaag
    32521 gatgttccaa taattgtact tattgtataa taccaaaact aagaggtaaa tatagaagta
    32581 gaaaatttga agatataatt aaagaagcta agaaattagc tgaatctgga gtaaaagaac
    32641 ttgtggttat agcacaggat acgacaaagt acggttttga tttatatgga aaagaaagat
    32701 tatcagaact tttagaagaa cttgcaaaaa tagatggatt taagtggata agggttatgt
    32761 attcatatcc agaatctata acagaagaat tgatacaagt tataaagaaa tatgataata
    32821 tatgtagtta ctttgatatg ccaatacagc atgcaagtaa caatatttta aaattaatga
    32881 atagaaaaac tactaaagaa gatatattaa ataaaatcaa tctgataaga agtaatatac
    32941 cagatgctat acttagaact acaataatag tagggtttcc tggtgaaaca gaagatgact
    33001 tcaaacaact tgtggacttt gttgaagagg ttaaatttga tagacttggt gcatttgcat
    33061 attcaagaga agaagataca ccagcagata gattgccaaa ccatatagat gaagaagtaa
    33121 aaatacaaag aagagatact ttaatgatga ttcaacaaaa aatatctgaa gaacttaatg
    33181 acaaaaagat aggaaaaact tatgaggtgt taatagaaga acagatagaa gataatgtat
    33241 atacaggaag aacacaaggt gatgcagagg aaattgacag tatagtgtat gtaaaaagtg
    33301 tagacaatct tgaagtagga gaatttgtaa gtgttcaaat aaatgatgca atggaatacg
    33361 atttaatggg agatgtttta tatgaacttg cctaacaagt taactttgtt tagaatattt
    33421 ttaataccag tatttgtttt aattatgttg ctcaatatac caaataaatt tttaatagct
    33481 tgtattattt ttataatagc ttcaattact gatgctttag atggaaaaat agctagaaaa
    33541 tacaatctag tcactgactt tggaaaattt atggacccat tagcagataa gttattggtt
    33601 atttcagcac ttacatgtat gatagaagac catcttgtaa gtagttggat ggtaattata
    33661 atagtggcta gagagcttac agttagcata ttaagagcta tagctgctgc tgatggaaaa
    33721 gtaattgctg ctggaaacag tggaaaactt aaaacgataa ctcaaatggt atctatagtg
    33781 tttttattgt taggtgctca gtttgaaaat gtattgatat taaatattgg tgaaattctc
    33841 attcttatag ctacactact tacattatat tctggatggg aatatctata taaaaataaa
    33901 gagcttttta tgtcttctaa gtaaaaaatt atgctttaat aaataaaaac taatgattaa
    33961 taaataaaaa ataacaactt aaaattttaa aaatgtaaaa aagggtatac tattatagta
    34021 tacctctttt cattttaaga atattttctt ttgtgtatat aattattaat aatggtttat
    34081 aaataaaatg taatgaataa aaatataatc attagtagcg tttttaaatt gactaggttt
    34141 gatttatgtt atataattaa tatataacaa gagaacaaat gtttgtagat aaatgaaggg
    34201 atggtataga gatgagtgta gatcaagaaa aattaaaagc gttgaatgaa gctttaggta
    34261 aaattgaaaa agattttggt aaaggttcag taatgaaatt gggagaagca acatctatgt
    34321 ctatagatgt tatatcaaca ggagcgattg gtttagacat agctattggt ataggaggtc
    34381 tacctagagg gagaatagtt gaagtatatg gtccagaatc ttctggtaag actactgttg
    34441 cgcttagttg tgtagcatca gctcaaaaag atggaggaat agctgcattt atagatgcag
    34501 aacatgcact tgacccagta tatgcaaaag ctttgggtgt ggatgttgat aacctaataa
    34561 tatctcaacc agatacaggt gaacaggctt tagagatagc agaggcattg ataagaagtg
    34621 gagcgataga tataatagta atagactcag tagcagcatt agttccaaag gctgaaatag
    34681 atggagatat gggtgattct catgtaggtt tacaagctag acttatgtca caagcactta
    34741 gaaagttaac tggttcaatt aaaaaatcaa attgtgttgc tatatttata aaccagttaa
    34801 gagagaaagt aggaataatg tttggaaacc cagaaactac tactggagga cgtgcactaa
    34861 aattctattc atcagttaga ttggatgtta gaaaaataga tacaataaaa caaggtgata
    34921 aagttatagg tagtagaact agagttaaag ttgttaaaaa caaagtagca ccaccattta
    34981 agcaagctga atttgatata atgtatggag aagggatttc aaaaattgga gaccttttag
    35041 atatagctgc tgatgtagat atagttaaaa aatcaggttc atggtatagt tacaatgata
    35101 ctaaacttgg acaaggaaga gaaaatgtta aaaaattctt ggaagataat ttagatttaa
    35161 ctactgaaat agatgagaaa gttagagcat tttacaattt aaatgaagaa catgaagaat
    35221 caggtacttc agtatcaaaa gaaattgtag aagaataagt aaaaatacaa aaaaataact
    35281 agtaattaaa gacatgccac gctccctatt agcttgacac aatgttgaaa aaattataaa
    35341 attaagtgag gataaaatat ctaaatttaa ttttaattat taggaatatt ttttaatcat
    35401 agtgatgaca ttttgggatt attaacgaag gaggtggatg tcatagatag tgtagtaatg
    35461 attgtaatag gtgcagctgt tggtattata gctggatatt ttgttagaaa aaatatatct
    35521 gaagcaaaaa ttggacaagc tgaaaacttg gccaaagaaa taatagataa agcacaccac
    35581 gactcagaaa cagtacaaaa agaaaaatta ctagaagcta aagaagagat ccataagtgg
    35641 agaacagaag ctgaaagaga aaacagagaa agaagaacag aagtacaaaa gtatgaaaaa
    35701 agagtagtac aaaaagaaga agtcttagat agaaaattac aaaatttaga atctaaagaa
    35761 gtaaacctaa gtgaaaaatt aaaggtagtg gaaaagaaag aagaagaagt tgaagtaata
    35821 aaaactcaac agttagaaaa attagaaagt atatcaggaa ttacttctga taaggccaaa
    35881 gaaatcatct taaccaatgc ggagagagat gtaagacgtg aaatgtctat aatgataaaa
    35941 gagatagaaa gtcaagctaa agaagaagct gataagaaat caagagaaat tataggatat
    36001 gcaattcaaa aatgtgcagc agatcacgta gccgaaacta ctgtaacagt agtaagcttg
    36061 cctaatgatg aaatgaaagg tagaataata ggtagggaag gtagaaatat aaggacttta
    36121 gaaaccttaa caggtataga ccttataata gatgacactc cagaagccgt aattttatca
    36181 ggatttgatc caataagaag agaaattgca agaattgctc tagaaaaatt aatagcagat
    36241 ggaagaatac atccagctag aattgaagaa atggttgaaa aggcgagaaa agaagttgac
    36301 aatattataa aagaatatgg tgaacaagct gcatttgaaa ctagtgtaca tgggattcat
    36361 ccggaattaa taagattatt aggtagactt aactatagaa ctagttatgg acaaaatgta
    36421 cttaaacatt ctatagaagt tgctcatata gctggtataa tggctgctga aataggagca
    36481 gatattagat tggctaagag ggcaggttta ttacatgata taggtaaggc tgtagaccat
    36541 gaaatggaag gtacacatgt tgaaataggt atggatttac ttagacgtta taaagagtct
    36601 aaagaagtta tacatgccat gagtactcat catggagatt acgagcctca aacagtagag
    36661 gctgtattag ttacagcagc agatgcaata tcagctgcta gacctggtgc gagaagagaa
    36721 actttagaag catatataaa gagacttgaa aaattagaag aaatagctaa ttcatatgaa
    36781 ggtgtagaaa aatcatttgc tatacaagct ggtagagaaa taagaataat ggtaaaacca
    36841 gaagctataa atgatgaaga aatacatcta ttggctagag atatgactaa gaaaatagag
    36901 gatgaacttg aatatcctgg acaaataaaa gtaagcataa taagagaaac aagagctata
    36961 gagtatgcga agtaaaagaa ataatatcta aataataaga aaatattttt agaaatttaa
    37021 atttgccaca agatatgata atatatcttg tggcttttaa attgtttaag tttaatttta
    37081 tataatgcaa aaataatcta agtgctaaaa gtgctactta caaaagatta ataaaataaa
    37141 gctttatata tgtattattt atataattat ccaagtttgt ttgatataat gaagttgtaa
    37201 aataaatata ttactacttg gaatgcttgt acttaaataa gatgaaaata caatgacgct
    37261 aaagtgagga ttagagagag ggtacactat gaaaaaaaat acacttttac atacaaatat
    37321 tatggtttgc attattatta ctcttggttt tgtggctacc tctataattg gatatcaatc
    37381 taataccaat atttttaaaa atgatattga acatgtatct acattggctg ctgaaggtat
    37441 ttattatcag atagataaac tactttcaga acctataaat gtatctctta caatggcaaa
    37501 tgacagtcta ttaaaaaatt ttttagatgg tgagaaagaa catttaaatg atgaagaatt
    37561 tatttataaa ttacaagatt atctgaatgt gtatagaaat aaatattcat atgattctgt
    37621 ttttttagta tcaactaaaa ccaataatta ttatcatttt aatggacttg atagaaatct
    37681 tagcgctaat aactcagaga atcaatggta ttatactttc ttaaagaatg atgatgaata
    37741 ctcattaaat gttgataatg atgaagcaag taacaatagt attacagtct ttgtaaattg
    37801 taagataaaa gatgataatg gagcaactat gggaattata ggtgttgggt taaaggttaa
    37861 ttcattacaa atgttgctta aaggatataa tgacaaattt gatgttgttg cacgcttaat
    37921 tgatgataaa gggtttgtac aattagcagt agataaaacc ggtcatgaga atataaattt
    37981 ttttgaaaat tctagtagcg acttgtctaa ttcgaaacaa ttaatattaa acaacaaaaa
    38041 ggaacaaaag agtttttggt attcatctga aaaatcaaaa agttatattg tatgtcagta
    38101 catacctagt ttaaaatggc atcttgttct agaaaatgat tttactttaa tgattaaaca
    38161 gcttcatctg caatttttta aaggcattgc agttatagtt ttaattataa tatttgtact
    38221 ttttactatt acacatgtgc ttaaaaaata caaaaaccaa ataataaagc ttagtgtttc
    38281 acagaaattg gaatatcaaa aattgttgag caaatctact gaaggtcttt atgaaaatat
    38341 ttttgaaatt gatattacta acaataaagc tggttgtgaa gatgagaata ggtattttca
    38401 tgaatttgga ttagggctta ataccccata tgatgaagcc ttacatacaa tagctaaaaa
    38461 ccaaatcaaa gaagaatatg cacaaggtta tttagataca tttttacctg aaaatgtaat
    38521 taaacgttat aatagcggaa ttaataatat cagctatgat tttttaatta aaaaagatga
    38581 agaaagttat cattggatta gaattaatgc acgaattttc ttttggacat cagatgaatc
    38641 tgttcgtatg atatcttatc gtaaaaatat agatgaagaa aaaaatagag aattactact
    38701 tctcgaacgt tcacaacgtg attctcttac agggctttac aataaagcta caacagagga
    38761 atttattcgt tcatttttaa aaaatgaaag taatcaagat ttagaacacg tttttctaat
    38821 tttcgatatt gataatttta aaaatgtaaa tgataaatat ggtcatgtat ttggagactt
    38881 tgttataagt gaatttgcct tagaattaaa atctcaattt cgtgaggacg atattgtggg
    38941 acgtattggt ggagatgagt ttgtagtatt aataaaagac tttaataata ataactatgt
    39001 gcttatagac aaattagagc gtttttgttc taagattaat cagaaaaaat ttgttgagca
    39061 agcagaattt tctattgcat gtagtattgg tgtagcaatg tatcctgaac atggatatgt
    39121 atactctgaa ctatatgaga aagcagacca agcactttac tatgcaaaaa gccatgggaa
    39181 aaattcttat catatatttt cagaggattc tattgatagt atttcatttc atatagactc
    39241 tagagatata aaagtattat tagaaatggc tactgatggt gttgtcaaat ttgcttgtat
    39301 ggaaaactta aaattacttt atttcaacca aaaaatgctt gaatttactg gatattcagc
    39361 tcaaactgtt acttctatag gttttgatat tttttcgcct gtattacaag acgacttaca
    39421 aatggtacta gatacattaa gagaagcaat ttctaaaaga gagacttttg aaatagtttt
    39481 tcgtttttgt catacagatg gtcatatttt tcctgtaagg atgcaaggta agtttgtaag
    39541 tgaattatat caaaatcgtt atcctgtttt ttatgcgatt tatacagaca tatcaagtat
    39601 caatacttta ctagaaaata aatagtaaat actacagtaa aatctaaaaa taggatataa
    39661 tagtattaat ataagaagat tataaataaa acctgctaat gaaaatagat tatttttagc
    39721 aggtttttaa attttttata ataaatctta tttttggcta acaagattta ttttttttaa
    39781 gactttactt attattttaa gcattaatta tgaaatttat cattttatta tttacagaaa
    39841 gtattttata acaataaata tctatactta ttgcaaaatt tttttagcag ttttgtattt
    39901 atttgtattt attttgaatt tatttactaa atttgcctat aaaaagtata tataaaaata
    39961 tagttcaaaa tggtataatt aagtataaca gaaggaggta atgtaagtga gccaatacat
    40021 aaatataaat tctcagttag acgccctaaa attgaaggag ttcgagaaaa tattttacga
    40081 aaatatcaat gaggacgtta aagtagtgcc aaaagttact ccttttaaag gaataaatac
    40141 cgatttgttg tatgttaaag atggaagaat tttatttata aaatttatgg atacaacaga
    40201 agatatattt tttattttag aagaagagtt acttgaagtt atgaatgagg aatatgaact
    40261 tttgaaacta aaaatgggac aaaagaatag aaatataagt tataactacg tttacattat
    40321 gccatacgtt gaagttgagg aaacatatga atttgaagaa tttgttaata ataatataat
    40381 agataaaaat aagcttcaag atataatgaa taagggttcg ctggaggaat atcttaatga
    40441 tgagaatgat gaaataaact taaatttatt tttactagac atatgttcag aatactatat
    40501 aataaatgat aaacttcatc taaatgaaaa atttaaaaaa atttcattct acaatgatga
    40561 ctataagtat acagcaacta tgatggaaga cgttcaaatt aaagatgttg tttctattaa
    40621 atatgggaat acacttattg aaggtggttc tggtataggc aaaacagcaa ttatgctttc
    40681 tagggctata aaattagcaa aagtatatcc acatcataaa ttagtcatat ttacacatac
    40741 aaaacaactg tgcaatgagc ttcgagagag gatagagtta ttatacaaag ataataacaa
    40801 tttagaagtt cacacattta gttcttttat atttaaacta gctaaaaaat tcaatttaat
    40861 tgtagattat aacatgttaa aaaatgatta cgaaaagacc tttaataatc ttgtaaaaca
    40921 agcacagaac attataaaaa ataaaaatat gttcaaggct atttttattg atgaagcaga
    40981 aagcttttta gagtatgaaa tagattttat aagggagttt ttatataaaa caaaattcat
    41041 atttaatgtg tgttcttgta actccttgaa tatctctaat agattgaata tatttaaaaa
    41101 attatataat ggtatagaat ttgatgataa gattatctta agtaaaaatt atagacaagc
    41161 taaagagatt gtagatttta caaataaatt ttcaaataat tctaactcat atatcaacga
    41221 attaagacca aatacagaat ttagtacttt cttttatact aaagcactta gaggtggaaa
    41281 taagtctgtt gatataataa aagtaagtga ccttgatgac caaataagtt ctgtaatctg
    41341 ggaaattgag tatttaataa gtaaaaaagg tcttgattat tctgaagttg ctatcgttta
    41401 tccttacaat aagaagaaat taaaaagtgg aaaaaccatt tactttcaat atatgttaaa
    41461 aaaagcattg gaagaggcaa aaattcctta tatatgtgca gaagataatt tgacaaatat
    41521 aagtaagaag gtaggaacta caatagcaaa tatatatgca ataaagaatt tagagtataa
    41581 agcggtaata gtatgtgaat tagagatgtt atacaatcaa actataaatg atattgaaca
    41641 agattatcaa gtaaatgact ttgtaggaga tttaaataag gtttatttag ctatgagtag
    41701 agcaacagac tacctaagta tagtaacaac ttttaatgaa gaagcaagtg atataataag
    41761 acttattaca gaatcaaaag atatttagta aaaaaatatt tgtaatttaa gaaaataaaa
    41821 agagactgag atttgtaaaa taaactttac caattcagtc tcttattttt tagtctactt
    41881 tcccaatatt atcaagaggt aatagtctca ccataacctt accaagaact tcatcttcat
    41941 caaccatacc tacatcaggg aatctactat cattactatt ttctctatta tctcccattg
    42001 caaatagttt accttttgga acaacagtat ctatatctcc agaagtatag ttattgtgta
    42061 tatatggttc atttaataat tttccattta catacacttt agaatttgat atttttattc
    42121 tgtcaccttc agtagctata actcttttta ttaagtcttt tttctttcct gtttctccat
    42181 caactaaatt ggttttaaaa actacaatat cgcctttttc aggtttacca accttgtatg
    42241 atatcctatt taaaatcaga taatctttat catctaaagt aggatacatt gattctccac
    42301 ttacaataga aggtcttata aattgtagaa ttacaaatgc aagtacaata gccatagcta
    42361 ttgacttaat ccaatcaaat atttcttttt taacactcat aacttttctc cttatcattt
    42421 gttattaaat tagaatacat tataatagaa agaaataagc aagaaccact atacccaaga
    42481 ctattctata ataaccaaat actttgaagt catgtttttt gatatagtcc ataaaaaatt
    42541 taataactac aattgaaaca acaaatgcta ctacagaacc aacacctaaa ataagccatt
    42601 caaatccagt aaatccaaat ccagctttta gaagttttaa tgcactagct ccaagcattg
    42661 tagggatggc taagaagaat gaaaactctg ctgctacata tctagaagta ccaagtaata
    42721 ctgcacctat tatagtagaa ccagacctag atgtacctgg tataagagct agacactgga
    42781 ataatcctat acaaagagct aatttatatg taacttgaga aaaatcttta tacttaggtt
    42841 ttttatttct cttttcaagc cctatcatta ttataccata tacaataaga gcaatagcaa
    42901 ctactgttga attaaagaat aatctatcta tatcatcttc aaacagtatt ccaataactc
    42961 cagatggtat aactgcaatt ataaccttaa gccataaacg gactgtttct tttttctgct
    43021 taatattctt tgcgctatca aaaggattta attttctaaa aaatataaca agtacagcta
    43081 atattgaacc aaattgaatt acaactaaaa atgtacttat aaatgtattt gaaaaattca
    43141 attttataaa ctcatcaact aatatcatat gccctgtact acttactggt agccattctg
    43201 ttattccctg aactatgccc aaaatgactg ccttaaaaat ttcaagcaaa ctaaatgttt
    43261 ccaaatcaaa atacctccaa tacaaattta tagtagcata ttttattcat agattaataa
    43321 aaaaatgcca ccttttacta acttataata ccatagtact gtgtagtgca caatggaaga
    43381 ttgatatttt cataaaacct tcataatata acaaaataaa cttaaaagtt atctattgaa
    43441 taaggggtat atttgagggg gaaatatatg gtatagtaaa agtatagcta ttttatttta
    43501 tgaaaaggaa aggagttatt atgtatagta caaaaatact agatgattat gagtatgaaa
    43561 cttttaatgt tttttctaca aaattaaatc caaacacgaa acacgattat ttatcaaagg
    43621 ttatactgtt taaagaattt ttaaaaggga aagaattgat ttatgctacg aaagaagatt
    43681 gtaaaaattt tgtagactat atacagacta aatatgcgaa atcaacttgt gaaaagatat
    43741 atagttattt acatagtttt tataacttct taaaaaaaga aggatacata gatataaatc
    43801 catttagata tgtagaaaaa cccacagtaa caagaataaa aacaaaagat gatgttctaa
    43861 gtatacaaga aataaataaa cttataggta tcctaccaaa attaaatata agagatagag
    43921 taataatagt ctgtctagtt actactgggt gtttattaaa tgaattagtt agcttaaagt
    43981 ggaaagacct tatggtagat gaaaacgata actcatatgt aaggttaggt aagggaagaa
    44041 aagaaagagt agttaaatta catccatact tttttaaact tttagaagat tacaggaatt
    44101 attctgggct tccagaggta ataataccta gtgatgattt tatttttaca actcaaaaga
    44161 gtaattctat aacagataga aatgtcagac ttatagtcaa aaaagcatta gatttagctg
    44221 gactttctca atattcagca agagatttta gacattcatt tgcagcaata agtttaaggc
    44281 ttggggcaga tgaaagtgat gtaaaaaatc agcttggatg gagtgataaa tactatgcaa
    44341 ttagatataa gtatgtatta aattttgttg atagtgaaat agtggattac ttaattgaaa
    44401 aagataattt ggatataaat aaaaaatagt aataaataaa aaatactata tataaatgtg
    44461 aacaaaaaat aaaaaagtct tgttatagtt cgtaaaatta tatataatta tttatgtact
    44521 aatgtgtaca cagtaatcac acgacaaaag atttaaggaa ggtgctaata taatgataga
    44581 taataaatat actacttact caaacccact tacagacaga tattgtagca aggacatgag
    44641 ttatatattt tctccacaat ttaaattctc tacatggaga aggttatggg ttgcacttgc
    44701 agaaggtgaa catgagttag gaatcaatat aactgaagaa caattagatg agctaagagc
    44761 taatatagat aatataaatt ttgatgaagc tagaaaaata gaaaaagaag taagacatga
    44821 tgtaatgtct cacgtaaaag cttatggttt acaatgtgaa aaagccaaag gaataataca
    44881 tttaggagca acatctgcat atgttggaga taatacagat gttatacaaa tgaatgaagc
    44941 tttaaaactt ctaagagtaa agcttgtaaa tttaataaac aatttaaaag aatttgcact
    45001 taagtacaaa gatatgccag ctcttggatt tacacacttc caagctgctc aacttacaac
    45061 agtaggaaaa agagcttgtt tatgggctca agatttaatt cttgatttgg aagatttaaa
    45121 ctatagaata gacaacatga agttaagagg agttaaaggt actactggta ctcaagcaag
    45181 ttttttaagc ttatttgaag gagaccacga aaaagtaaaa gagttagata agaaaatttg
    45241 taaaaagatg ggataccatt cttcctatgc agtaagtgga cagacatata ctagaaagtt
    45301 agattatcaa gtaataagca tactatcagg gatagctcaa agtatgcata agatgactaa
    45361 tgatataaga cttttacaaa gtttaaaaga attagaagag ccatttgaaa aaaatcaaat
    45421 aggttcatca gcaatggctt ataagaggaa tccaatgaga agtgagagaa tagcatcttt
    45481 atctaagtat ataatctctg aatctataag ccctgcaatg gtacaagcca ctcaatggtt
    45541 agagagaaca ttagatgact cagccaataa gagattggca atacctcaag catttatggc
    45601 agcagatgct atacttgaaa tagggataaa tgtaactgat ggtttagtag tatatgaaaa
    45661 tatgataaat agacgtgtaa atgaagaact tccttttatg gctacagaaa caatacttat
    45721 ggaagctgta aaaagaggtg gagatagaca ggaattgcat gaaataataa gagaatattc
    45781 tatgaaagct gcctatagag taaagcatga aggcaaggat aataacttaa ttgaacttat
    45841 aatgaatgat gattcattta aaatgagcaa ggaagaaata ctatctataa tggacccaaa
    45901 aaactttata ggtagagctc cagaacaagt tgtagaattt gtaaatgaag ttgtagaacc
    45961 tgctataaaa gattataagg aagacttagg aaaagtagat gtagacctaa gagtatagaa
    46021 ttgtacagat aaaatataat taaaaacggc tatcttattt ataaataaga tagccatttt
    46081 aatgggctag cgtcttctct ccaccgctgt gaagagttct tctttactta ctataccatg
    46141 aatttaataa aatagctata taaataatat ttaataggaa taatataaat aaaaataaaa
    46201 aaatgataag attactaatt aaatttaaac attcagcaaa aactattgta cttgcaatat
    46261 ttttaatgta aactgaaata aagttattta gataatttaa taaattttaa aacaaccgag
    46321 gtgattatat gaactatata gtaaaagata ccataagttt tttagtaaaa acatttccta
    46381 agatttattc tagcctgtac ttagaagacc taaaaaaatt tgcaccagat tataatgtaa
    46441 ataaaacaca attaagagca ttagttttca taaaaaatta tggagtaata agtatgactg
    46501 atttatgctc taagttaaat atagaaaaag gcagtttaac tagtatggtt gatgatttaa
    46561 ctgataagaa gtatgtaaca aggaaaagag atttagttga tagaagaaaa tatctaattg
    46621 acattactga agagggagat aagatagcaa ctgattttat ggataaatta agtgatggtt
    46681 taggagaaaa actttctaaa ttaactgaag aagacagaaa aaaatattta gaagcaataa
    46741 atactcttca atatatatta aacaaagaag aatttagata gaaaaaatat aaaaattttt
    46801 gtatttaatg gtactaagat taattctttg ttattcattt aactatataa ttataagtct
    46861 aaatacaatc taaaaagtac atcttaaatt ttcttgatgt acttttttat aatttctcta
    46921 actaaggcaa gacaagtttt agatgcttta attaaagtga ataaagatta ctggccaaca
    46981 ctatataaag aaaatgaaaa atagtcaggt ctaagtctta attaatatat taattaagaa
    47041 atgtcccctt ttcaaataat atctgaaaag gggacatttt atatcattaa atttatataa
    47101 attaagttta aaaaattatt tttgtaaagc tacatattgc tctaaacctt cttttaataa
    47161 tgtagcagct ctatgaaggt ctttttcatt taagatgtaa gctaatctta tttcatcacg
    47221 tcctaaacct ggagtagcat agaatccttc agcagggcaa accataactg tttcattatc
    47281 tttattaaat tcttttaaca tccaaattgt gaaatcttct gcattttcaa ctggtaattt
    47341 agcaactata tagaaagcac cagttggttt tttacatata acaccttcaa ccttcattaa
    47401 ttcattgtat aatacatctc ttcttttttt gtactcttca ttaacttctt tgaagtaaga
    47461 aacaggagtt ttgtataact caacagctcc tatttgctct aatgtaggaa cacaaagtct
    47521 tccttgacat aatttaagta tttgagcgat aagaccttta tttttgcaag cgatagaacc
    47581 aattctagca ccacaagcac tgtatctctt agaaacactg tctattatta taactctatc
    47641 ctgtacaccc tctaagttac cacagcttgt atactcaagc ccatcataaa caaattctct
    47701 ataaacttcg tctgctatta tccataaatc tttttcttta gctatttcag ctaggatatt
    47761 aagttcttct tttgtataaa ttgcaccagt tggatttcct gggttagaaa gaattatagc
    47821 cttagtttta tcatctactt tagacaatat ttcttcttta gatggaaggt ggaaaccgtt
    47881 ttctgccttt gttgtaacag cattaacttc tacgtttaca gattgaccaa atccattgta
    47941 gtttgtatag aatggttctg gaactaatag attatctcct gggtcacaaa ctgccatcat
    48001 agtgaaaagt aaagcttcac taccaccgtt agttactaat aattcatctt tttcaaaatt
    48061 catatcgtaa gttttatagt aattttgaag tgcttctaat aattctggaa taccttctga
    48121 tgtagcatat tctaagactt cgctatcaaa gtttttaaca gcatcaaaaa atcctttagg
    48181 agtttttatg tctggttgtc ctatgtttaa gtgatatact ttgattcctt tatcttttgc
    48241 tgcttgtgca aatggaacaa gttttctgat tggagaggct tgcattgcac taactctgtt
    48301 tgaataattc attttaatac cccctattta atttaaatac gtatattgta ttatgtaaat
    48361 tgtatgtttg aatacaagac atctttacaa gataataata acatacttat gggtaaaaat
    48421 catcaaaaaa aattaatata gctttaaaat aaacttttaa caaattatag taaaataccc
    48481 atgtttatgc cactaaatta tgtattcata aattaattta tagtaagata atattctgat
    48541 gtactgatag tgatttttta gttataatct ttcagctata gacaaaatat ataataataa
    48601 atatgcaaaa ttataaaaaa tattataaat ttatttaaca gatacgataa atatcatata
    48661 attatattaa aggctattaa ctaagaggtg ttaattatga aagaattaag aatagatcca
    48721 attaccaatg atgttgttat atttgctaca gataggttaa aaagaccttt agatacagca
    48781 gatatcccaa atgaagatga agaaactaat gaatatgatg aagaatgccc attttgcaaa
    48841 ggaaatgaag ctcatgcaac tgatgcatta tttgaaatag atagtgaaga aggatggctt
    48901 gtaaaatctg tctacaataa atttcctata attgatgata tggcaagaga tgtatatggt
    48961 gttcatgaag taatgataga gagcgataaa cataatcgta gtttttataa catgagtcaa
    49021 aaagaatttg aagatgtttt ttttatgtat agaaatagat ttagagattt gtcgaaagat
    49081 gataaagtag agtatgttag tatcttcaaa aacttcttga gaaaggcagg agcatctttg
    49141 atgcatccac atgctcaaat attgtctatg tcttttattc cacctgaaat tacgaatgaa
    49201 ctgttggttt caaaggagta ttatgacagc aataaaagct ctttgtatga tgatttaata
    49261 gaaaatgaaa taagtttaga tagaagagtt gtttataatg gagaagcttt tttggtactt
    49321 ataccatatg caaccaagta tagtggagaa gtaagaataa tatttaaaga taaaatagag
    49381 tttggtgagt taaatgataa taatatacag gagctttcta caatatttga aaagttgttt
    49441 aagaagttat acaatatcaa tggatatatg ccatttaatt tatgtattca cactcatcca
    49501 acaaagattg aaacaaaatc gtattttaat gttcatatgc acataatccc aagaaaatat
    49561 aattttggag gatttgaact tggaacaaat atgtatgttt cttcaatgag ccctgaagat
    49621 ttaacaaaaa agttaaaaat tgattaaaac atatattttt aggtattaag taaatagtaa
    49681 ttttagatga aattatagta tagcattgtt aaaataaaag taaattaaga atttacaata
    49741 aaattgttga aaagccccca tctttagcta tggggagtgt gtcaatgttt agatgaggag
    49801 tataagtatg aaatttatat catggaatgt taatggaata agagcttgtg ttgggaaagg
    49861 atttttagac tttttcaaag aagtagatgc agatatattt tgtttacaag aaactaaatt
    49921 gcaagaaggg caaatagagt tagatttacc aggatatttt caatattgga attatgctga
    49981 gagaaagggt tattcaggta ctgctatatt tacaaaaaaa gagcctttaa aagtaatgta
    50041 tggaataaat atagaagaac atgacaaaga gggtagagtg ataactctag agtttgaaga
    50101 tttttacttt gttacagtat atacgccaaa ttctcaatct gaattaaaga gactagaata
    50161 tagaactaga tgggaagacg actttataga ttatttaacc aaactagata atcataagcc
    50221 tgtaattgtg tgtggagata tgaacgttgc acataaagaa atagatttaa aaaatcctaa
    50281 aaataatatg aaaaatgcag gttttactaa agaagaaaga gagaagtttt ctaaattatt
    50341 agacagtggg tttatagaca catatagata ttttaatcct gataaagaag gtgtttatag
    50401 ttggtggtcg tatagattta atgcaagaaa aaataatgca gggtggagaa tagattattt
    50461 ttgtgcatcc aaaaaattag aagatagatt agtaagtgcg gatatacaca ctgaaatatt
    50521 aggttcagac cattgtccag ttgaattaga gataaaataa aaattaatta tgataaaaat
    50581 tgggctgtag atataaattt aaagttaact agttttttaa tctacatgcc tattttttat
    50641 ctaaataaac tctatttgtt ttttctttgc ttaacatttt ctaatgtaaa ttgatttgtt
    50701 ttccacatat tataagctaa gttaatgatt tttttctcgt ctttagttaa ttcacgattt
    50761 aaaattttcc taaattcatc aaataactta tcataatcaa ctcttttttt gttagaatat
    50821 cttatagttt taggttttat actcgtataa agatatatat ctgggactat acttggattg
    50881 tatatcttaa taaaaacttt agcagtataa tatgcatcat ttaaagcatt gtgataagac
    50941 attttttcat caagttccaa tatagaaata gcattttgta ggccaatgct ttttcctgct
    51001 ggattattga aatatttaga tgcatgttgt tgtatattaa tataagattt aggtaaggaa
    51061 tttgatggta gcttgtgata gttgatattc ctgtataatt ccttcaaatc tccagctccc
    51121 caaacacata atatagggtc ttgtgatgat ataaatttta taaaattgtt gtagacctca
    51181 gggaaagtag gcgaatcatt aaaatcagaa cttttaatat tagtcatacg actaataaag
    51241 ggatgtatgt ctttataaat tgtaggtttt acatagctat taaacgtatc tattatattg
    51301 aatttagaat ccaatttaat tgctcctatt tgtataattt caaaaggaca cttttcattg
    51361 gatacagttt tattgagttt tttatcaaat ccttggttaa attccaaatc gaaaattatg
    51421 tattccataa agtttatctc ctaaa
//
LOCUS       DAYAPA010000012        50331 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.012, whole genome shotgun sequence.
ACCESSION   DAYAPA010000012 DAYAPA010000000
VERSION     DAYAPA010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 50331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50331
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.012"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(<1..1388)
                     /locus_tag="ACGI59_000614"
     CDS             complement(<1..1388)
                     /locus_tag="ACGI59_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HFG9738422.1"
                     /translation="MYKLIDKKDIDFLIDTCGEENVLVGSDINEDFSHDELGGIEKYP
                     EVLVNVLETEQVSKIMKYAYKNNIPVTPRGQGTGLVGAAVAINGGIMINLCKMNKILE
                     VDYENLTLTVEPGVLLMTIGQYVQDRDLFYPPDPGEKSATIAGNINTNAGGMRAVKYG
                     VTRDYVRGLEVVLPNGEIINVGGKVVKNSSGYSIKDLLVGSEGTLGIVTKAILKLLPL
                     PKKSISLLIPFPDLSMAIETVPKIIKSKSIPTAIEFMERDVILAAEEFLGKKFPDNTS
                     DAYLLLTFDGNSTEDIEKEYEKVANLCLENGALDVFISDTQERNDSIWSARGAFLEAI
                     KASTTQMDECDVVVPRDKIAEFIRYTHELQDKLKIRIKSFGHAGDGNLHIYILKDGMD
                     DNTWKIRLKETFDYMYKKSRELSGQVSGEHGIGYAKKEYLHESNSDAYMMLIKNIKLA
                     FDPKNILNPGKI"
     gene            complement(1399..2619)
                     /locus_tag="ACGI59_000615"
     CDS             complement(1399..2619)
                     /locus_tag="ACGI59_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HFG9738423.1"
                     /translation="MSKIVVNQDKITDLKRILEICPFGAIEEKSGIVEISAGCKMCKL
                     CVKSGPKGAFEFIESSKVQINKDEWRGIAVYVEHHNGNIHPVTYELIGKAREMASKIK
                     QPVYCVFVGKDIKDKCSNLISYGVDEVFVYDEDEFKDFRIEPYSKAIENFIDKIKPTI
                     VLVGGTTLGRSLAPRLAARFRTGLTADCTILDIQSNTDLDQIRPAFGGNIMAHINTPN
                     NRPQFATVRYKIFSAPEKIENTTGKITLCKVEKKELKSKIKVLSVKEKNKEVGLEEAE
                     VIVVASRAIKKQEDMEMIYKLADKLNAQVAGTRPVIEAGWIDAKKQIGLSGRTVKPKL
                     IITCGVSGAVQFVAGMQGADYIVAINKDDKAPILDVAHLALIGDIYDIIPKLIEKIEN
                     NKDNNQKYMASAAK"
     gene            complement(2633..3421)
                     /locus_tag="ACGI59_000616"
     CDS             complement(2633..3421)
                     /locus_tag="ACGI59_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HFG9738424.1"
                     /translation="MEILTCIKQVPGTTSVEVDETTGVLKRDGVDSKMNPYDLYALET
                     ALRIKEDKKANLKVLSMGPPQAKKVIEESFMMGADEGALISDRRFGGADVLATSYTIS
                     QGIKKMGKVDLIICGKQTTDGDTAQVGPEVAEFLDIPHVTNVTKLIEVKDESIVVEID
                     MPNDLQVCEIEYPCLITVEKDIFQPRLPSFKLKLNTKDREIPVYSLDDFEDKDENNYG
                     LNGSPTQVVRIFPPKPNTDKNIVRGNADELSFALVNKLEELKLV"
     gene            complement(3438..4712)
                     /gene="larA"
                     /locus_tag="ACGI59_000617"
     CDS             complement(3438..4712)
                     /gene="larA"
                     /locus_tag="ACGI59_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HFG9738425.1"
                     /translation="MVKLKVPYSKQGMILEIPDERFLGVLESKSDNYIEHKTQEEIVE
                     ESMNNPIGSNSLEELVKGKKNVVIITSDHTRPVPSRITMPIILRRIRNVNPEIDVKII
                     VATGFHRPSTREELIYKMGEEIVDNEDIVMHISTDDKSMCKVGILPSGGDLYINKLAY
                     EAELLIAEGFIEPHFFAGFSGGRKSVLPGIASAKTIMYNHCSDFIDSDNSRTGKLNNN
                     PIHEDMVYAAKVAKLAFILNVVIDKDKKIIASFAGDVEKAHTKGCRFVTELSKVNSIK
                     SDIVVTTNGGYPLDQNIYQSVKGMTAAEATCKDGGVIIMISACNDGHGGQSFYENVAN
                     ADSPKQLLDKIRSVDRVDTIPDQWEFQILARILSKYTVIMVTDKCNPEMIKKMHMKHA
                     FNFKEALEMANSIVGKESKVVVIPDGVSVIVK"
     gene            complement(4830..5543)
                     /locus_tag="ACGI59_000618"
     CDS             complement(4830..5543)
                     /locus_tag="ACGI59_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFG9738426.1"
                     /translation="MFNSIASKKVYEQVIEQIQYKILNGELKKGDKLLSERELSEQMN
                     VSRTSIREAIRVLETMGVIESRQGEGNFICTNIEKTLIEPLSMIFKLNNGTLEDILEL
                     RIILEIEIAKLASKRITSSEVIELKHIIDEMRVETNKKDNNRVLVLLDQKFHSKLATL
                     SKNYLIQSLFMTASKLFDGFIEDAREKIIAEPFNENILLKQHEAIYNAVVENDVELAC
                     EKAKEHMDFISKNYRKNEN"
     gene            5668..6927
                     /locus_tag="ACGI59_000619"
     CDS             5668..6927
                     /locus_tag="ACGI59_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YlbJ"
                     /protein_id="HFG9738427.1"
                     /translation="MKKNKIIAFFAPSIIVGVLVLGIILYPNEAIKSAREGFSIWSNV
                     LVPSLLPFIIGANLIVDLKIVDIIGFIINPITRFVFNVSGKSALAFAISTVSGYPVGV
                     SLASEFRSNGQISKYEAQRLVSFCSTSGPLFIIGSVGTGMFQNSNLGYLMILCHYLGT
                     ILVGLFFRNYGNENLPKTKLDLKTNIKNVINIRNSSGNGFFVLFGNAVFNGVNTLLTV
                     GGFVIVFSVVFKILSLFNVISLIASVIYLPLSFLGVSKELCQAFVSGLFEITIGCNKV
                     SSITSSPEILRASLASFLIGFSGLSILAQCCTFLSKTDIDLKLYILSKFAHGVLAAIF
                     IFILYPIANSAVLVSSFADTYNVIYNNLIWFYYLSYYDTILQIVVIIYLISAVFIAKK
                     HMKTLSTNGKLMRYKNTFFTKKFSRKK"
     gene            7207..7773
                     /locus_tag="ACGI59_000620"
     CDS             7207..7773
                     /locus_tag="ACGI59_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFG9738428.1"
                     /translation="MATRPIEIDEYKKIMELLHTGFTYSENGVEKRFRKNPKVALALM
                     LEANLGLRISDILRLKIGNFKGNMLEINEKKTGKLQYRPVNKNIIESINEHARKYNLK
                     SNDYLVNIKTKAIQKQLRIICKYLNLYNISTHSFRKLYATTQFEKSNNNLELVKELLN
                     HSSVATTQRYIRVTQQAINEASENFFIG"
     gene            complement(7974..9179)
                     /locus_tag="ACGI59_000621"
     CDS             complement(7974..9179)
                     /locus_tag="ACGI59_000621"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HFG9738429.1"
                     /translation="MKIAILGYGTVGSGLIDLIENNTSKRDIEVVGILVKNKEKHKYK
                     KYFDKITTDIEDIFDKDIDILVEVIGGLNPAFDYVTRALNKKIHVVTANKDLLAEKGS
                     DLIELANLNNVSIKFEASVAGGIPVLKPLIESLEGNNIKSINAILNGTCNFILSKMYD
                     ENLPYDVALKQAQELGFAEVNPDADVLGYDSARKLSILSTLSYGKIVYWKDLLLEGIT
                     SIDEKDIEYAKKLNCKIKLVARSKYESGEVSGFVRPALVDNNNMLSKIDNEFNVVILV
                     GDSVGELSFVGKGAGRGATGSAVYADIIDIIDNRSSNIKSFSKGKLDLSGLIEDECSA
                     VVRFGGYNNKDGILECLDKYVDSYDIIDDEELAIFVSAKSEHEIDRVLEIVKRNNYSE
                     SAKKLLKID"
     regulatory      complement(9282..9388)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(9480..9848)
                     /locus_tag="ACGI59_000622"
     CDS             complement(9480..9848)
                     /locus_tag="ACGI59_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbY family RNA-binding protein"
                     /protein_id="HFG9738430.1"
                     /translation="MLKGKQRAYLRSLANTLKPTTQIGKEGVTESFLEQLDGMLRTRE
                     LVKVTILENAGLDTKETANAVCEALRAEFVQAIGFKFTIYKRNIEEPKILFPGHEQAK
                     AKTKNNNVTKKGKPTKRAVR"
     gene            complement(9900..11177)
                     /gene="obgE"
                     /locus_tag="ACGI59_000623"
     CDS             complement(9900..11177)
                     /gene="obgE"
                     /locus_tag="ACGI59_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HFG9738431.1"
                     /translation="MFIDKARIFVKAGNGGNGSVAFRREKYVPAGGPDGGDGGRGASI
                     IFEVDLGLRTLMDFKYQKKYQAQNGGDGSKGKRAGKNGENLVLKVPAGTVIRDEATGL
                     VLADLKKEGDTAIVAKGGIGGKGNQHFANAVRQAPAFAKSGTDGEERWITLELKMIAD
                     VGLLGFPNVGKSTFLSVVTKAKPKIANYHFTTLTPNLGVVQTKFGDSFVLADIPGIIE
                     GASEGIGLGHEFLRHVERTKVLIHIVDISGLEGRDPIEDFDKINDELKLYNEKLSKRP
                     QVVVANKFDILEDESKFEKFKSELEGRGYTVFKMSAATRQGIDEVIAYVSKMLKEVED
                     VELVSEEEMYRPELDIGTEEELSIDIEDGVYVVTGKALRRIMYSVNFDDMESLQYFQK
                     AMESQGVFDRLREMGIEDGDVVKIYELEFEFYN"
     gene            complement(11288..11578)
                     /gene="rpmA"
                     /locus_tag="ACGI59_000624"
     CDS             complement(11288..11578)
                     /gene="rpmA"
                     /locus_tag="ACGI59_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFG9738432.1"
                     /translation="MLNMNLQLLASKKGVGSSKNGRDSISKRLGVKRFDGQLVTAGSI
                     IVRQRGTKIHPGTNVGKGSDDTLFALVDGTVKFERKDKKRKKVSIYPVAIAE"
     gene            complement(11592..11909)
                     /locus_tag="ACGI59_000625"
     CDS             complement(11592..11909)
                     /locus_tag="ACGI59_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HFG9738433.1"
                     /translation="MIKVKYYYNDDFLLQGFCLKGHADFAEIGYDIVCAAVTSNAIAV
                     INSLDKLQKIEFEKVVGEEGHIECIVKDTHVKDAQLLLNHFQLAVKEIKREYPKNIKI
                     LKK"
     gene            complement(11924..12235)
                     /gene="rplU"
                     /locus_tag="ACGI59_000626"
     CDS             complement(11924..12235)
                     /gene="rplU"
                     /locus_tag="ACGI59_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000270907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFG9738434.1"
                     /translation="MYAIVKTGGKQYKVSEGDVLFVEKLEANAGDVVTLNEVLACSKD
                     GELKLGSPVVEGASVQAKVVEQGKAKKVIVFKYKAKKDYRRKQGHRQSYTKIVVEKIN
                     A"
     gene            complement(12336..13757)
                     /locus_tag="ACGI59_000627"
     CDS             complement(12336..13757)
                     /locus_tag="ACGI59_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HFG9738435.1"
                     /translation="MKKIVIESLIGSQKTAVLEDERLTELFVEDNLNKKTVSNIYRGI
                     VKKVIPGIEACFVDIGFKKLAYLQLKKGSTIKSGQDILVQINKEEIGTKGAKLNTEIS
                     ISGRYIVYIPSNDRTTISNKITDEKERFRLKKITKAVNKENLGLIIRTEAQGCTHDEI
                     KKDIEELKLKYENILKEYKLGIGPKLLYKSLDFATKYVKDNVNDDIESIITNSYDKYS
                     ELKSILRGIDKTYVDKLCLEENRDVFDLYRIESKIEKLLNKKVWLKSGGYLIIEKTEA
                     LTVIDVNTGKFIGTGKLDETVYKTNLEAAKEIVRQLRIRDIAGIIIIDFIDMHKKKHQ
                     KEILHILEEEFNKDKRKAEVLGMTKLGLVEVARRREKESIDKYYLMSCPCCDGEQTIK
                     SVHYILDSIEKEIMRISEHTVYKNIMVEFNDFIFEQIKEYYMDIIDKIGEKYNIKISL
                     NANSTLKHNKTNVIFDKIVDNKM"
     gene            complement(13775..14476)
                     /locus_tag="ACGI59_000628"
     CDS             complement(13775..14476)
                     /locus_tag="ACGI59_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HFG9738436.1"
                     /translation="MSKIIRVKFKKEGDMIYISHLDLQRLLQRAFRRAEINLSHSQGF
                     NPHPKMSYGNALALGAESQGEYVDIEIEEDDLSVEEFLNKVSIQLPDGIDFIKAKEID
                     RQTPSLSSVIDYGEYLFNIDLKRPMTKEFVKRKVIDFMNNKEIIITKKNKKGKMVEVD
                     IRPMIRTFDVLNLEDEHITLTATIATGSKTNLNTNILIPKILEMFELDIDPLDVDILR
                     RDLYVLEDGELVTPM"
     gene            complement(14478..16322)
                     /locus_tag="ACGI59_000629"
     CDS             complement(14478..16322)
                     /locus_tag="ACGI59_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HFG9738437.1"
                     /translation="MNKVDLKKILKKVEKPARYLGNEINSIHKDTSNSELIRYAHCFP
                     DLYEVGMSHLGSHILYDVINKDEDVFCERVYSPAVDMENIMREKSIPLFALESREPIT
                     NFDFVTFTLQYELSYTNILNILDLANIPILKEERTLEDPFIMVGGPCAYNSEPLADFV
                     DVVILGEGEEVNLEVVNEYKEWKKNKTTREDFLYKISSIEGVYIPSFYDVKYNEDGTV
                     QSVTPNREGITKNPTKRIIKDVETVDYPEKLIVPYIDTVHDRIVLELFRGCTRGCRFC
                     QAGMIYRPIREKSVKRLKEILDKLVKNTGYDEISLSSLSTSDYSKLSELTDYLVDEYA
                     SNNIGISLPSLRLDNFSMEIADKIQQVRKSGLTFAPEAGTQRLRDVINKGVTEEDLEN
                     ATERAFEMGWNSVKLYFMIGLPTETYEDLDGIAKLAYKVIDIYRKVNGGKLKRSFSVT
                     VSTSTFVPKPFTPFQWHGQDTTEEVINKQRHLVNKLRNNNIKYNYHDSKTSLMEAVVA
                     RGDRRIGKVIYDAFRLGAKFDGWAEHFNLDIWKEAMEKNNLSIDFYAHRNRNYEEVFP
                     WDHIDVGISKKFLIREDENAKKEKITSDCRHNCNGCGINIHDIGRGLC"
     gene            16641..17834
                     /locus_tag="ACGI59_000630"
     CDS             16641..17834
                     /locus_tag="ACGI59_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HFG9738438.1"
                     /translation="MSKVFEVKKDIYFTGVVDEGLKVFDIIMETEFGTTYNSYLIKDE
                     KTVLFDTVKANFKDEFLSNLSEVTDIAKIDYVVIHHTEPDHAGSLKYLLDINPNIEVY
                     CTKAAKLYLDGQINRPFNCHVIKDGEILNIGKRNLRFITAPFLHWVDTMFTYIEEDKT
                     LLTCDAFGCHFASVDAEVVNSEDYLKSAKHYYDCIVKPFAKHVLSAVDKVVGLNIEFD
                     TILTSHGPMLTKDPMAAVKRYVEWSTEAVNTTNQNQVSIFYLSAYSNTLEMAKKIKEG
                     LDKEGAKAELYDLEDMTLTEMHDTLVVSKVILLGSPTINKTMVKPMWDLFSVIDPMAN
                     QGKIAGVFGSFGWSGEGITMAETLLKSMSFKMPVESLKKKFFPSEETLKECMAFGAEF
                     AKLVK"
     gene            complement(18046..18866)
                     /locus_tag="ACGI59_000631"
                     /pseudo
     CDS             complement(18046..18866)
                     /locus_tag="ACGI59_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428476.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(18996..19787)
                     /locus_tag="ACGI59_000632"
     CDS             complement(18996..19787)
                     /locus_tag="ACGI59_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HFG9738439.1"
                     /translation="MKLIDEYLDKLYKKCDNKSTIELKQEMRCHLIESANEFKLEGLD
                     EEEACKKAIERFDDGDEMQYELCNIIKELSLSLDRHKSIVMGFKKVLGYISIIAFLIS
                     GFMWYYNNSLQHNMYNLGKELDGEIKQLAERHDMTKIGEYKLELEKILDKDKYSKVKA
                     LRLYVIDMKDGNTNLSSSGLNANMVYEREADYNNISNFIQHLGYNGKDFLDKNGNIVN
                     PDIFLEYFFYFESEMLIPVAFVFGLLCIIAYFILRFKISLIKNNN"
     gene            complement(19791..20120)
                     /locus_tag="ACGI59_000633"
     CDS             complement(19791..20120)
                     /locus_tag="ACGI59_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFG9738440.1"
                     /translation="MQLNKEVLKGYIDILIVSILEKKDCYGYEIAKQVRERSEFELKE
                     GTMYLALKRMESKNLIKSYYSNEQSSGGRRKYYNLTNEGKDFLEIKKQEWRFIKKVMN
                     QFLGEVD"
     gene            complement(20605..21018)
                     /locus_tag="ACGI59_000634"
     CDS             complement(20605..21018)
                     /locus_tag="ACGI59_000634"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFG9738441.1"
                     /translation="MNIALVAHDQMKNTMVGFCIGYESILKKYGLYATGTTGKRIMDE
                     TELNINRLASGPLGGDQQIGSLIVTQEIDLVIFLRDPLTSQAHETDIQALIRLCDVYH
                     VPIATNLASAEIFIKALDRGELSWREVRKSKSQRI"
     gene            complement(21059..22189)
                     /gene="rodA"
                     /locus_tag="ACGI59_000635"
     CDS             complement(21059..22189)
                     /gene="rodA"
                     /locus_tag="ACGI59_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HFG9738442.1"
                     /translation="MKKININYKSTIKLIKQLDWKLIVTVLAIFIFGLVILSSATHAN
                     STGSYNQLIKQGLAFVLGIGMIIVILFFDYNLLGRYYKALYIISLILLAIVLLPGIGT
                     VKGGARSWINLGPLDLQTSEIVKLTFVLSYAKILESKKDKLNTLKEVMPVVVYSLPFI
                     GLLIAQPDLGTGIVFCCMIFAMLFTAGLSSKLIKRGIIILLVSMPLMYLMMADHQKVR
                     IEAFLNPEDVTLKGNYQVMQSLIAIGSGGVTGKGLYNGSQNQEDFLPVQDSDFIFAVV
                     GEELGVIGMAVLIILFMIFLLRLLAIARDAKDFYGTLIVVGVMGMFGYQIIQNIGMTV
                     ALIPVTGVTLPFVSYGGSSLLTSLANLGLVLNVCMRRKKINF"
     gene            complement(22274..22558)
                     /gene="minE"
                     /locus_tag="ACGI59_000636"
     CDS             complement(22274..22558)
                     /gene="minE"
                     /locus_tag="ACGI59_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HFG9738443.1"
                     /translation="MLDLFRVFSNEAKTSKSVAKERLKLVLVHDRVDCSPQLLEMIKT
                     DILKVIANYAEIEDDGLEIKMSKCRGEHDDKPVSALVANIPLKNIKDRCM"
     gene            complement(22576..23373)
                     /gene="minD"
                     /locus_tag="ACGI59_000637"
     CDS             complement(22576..23373)
                     /gene="minD"
                     /locus_tag="ACGI59_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HFG9738444.1"
                     /translation="MSEVIVITSGKGGVGKTTTAANLGTALSLENKKTVVVDADIGLR
                     NLDVVMGLENRIVYDIVDVVEGTCRLKQALIKDKRFDNLYLLPAAQTRDKNAVSVEQM
                     IDLCEKLKESFEYIIIDCPAGIEQGFKNAVAGADRAIVVTNPEISAVRDADRIIGLLE
                     ANEIKEIRLVINRIRNDMVKRGDMMDKQDIIEILAIDLLGLVPDDESIIISTNKGEPA
                     ILDSKSLAGQAYKNIAKRILNEEVPLLDLEVEDGFFGRLKKMFSMAK"
     gene            complement(23397..24080)
                     /gene="minC"
                     /locus_tag="ACGI59_000638"
     CDS             complement(23397..24080)
                     /gene="minC"
                     /locus_tag="ACGI59_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA];
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HFG9738445.1"
                     /translation="MSLREICSQELVEFKGNKRGIIVNIKREAPFEEIQEKIINKLEA
                     YVGFFNGAKISKINSDCLTDMEILELKEGITSRFDVEFVEDQKIEENSNFPTKYVNTL
                     RSGENIEFEGDVVILNDMKPGSKVLSKSNTVVMGDINAGAKVVAGGNVFVMGKIEGFV
                     HAGAEGNEFAYVVAGNLNPKILQIADNIAEAPDDEENYESESEISPEIAFVSNGRIVI
                     ESYLSKLDK"
     gene            complement(24178..27156)
                     /locus_tag="ACGI59_000639"
     CDS             complement(24178..27156)
                     /locus_tag="ACGI59_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFG9738446.1"
                     /translation="MEENKQVDRLKIIKIVMGVIFLAILVKIIYMTTFKYEYYNELAE
                     NKTYKKLAIEAPRGEIKDRYGRLLAGNKNLFTVQVSGNDINKKDANKHSRANEISLKL
                     INLLERNGEEYVDEFPIYVENGKYYYTYDRDIREYKSENGIPNDYNAKESFYYLVDKL
                     ISAGILSQEDKRLDATRLQAKLNENGYYPPILVSKWMFTAERDKRDWLASYKIKETKL
                     SAKEAFEKVRNSDALEIDKSLSDEDARKIMVVRDLIKSKGYSQYNPVTIAKDVGETTI
                     AQIEESAMDLVGVSIAVEPVRYYPNGSLASHMLGYVGKMPSTQIESYLQKGYETGDMV
                     GLAGVEKSNESRLRGTDGYKMVKVDALGRISKEIESKKPKSGDTVYLTLDKDLQEVSD
                     NALKQIIEVASKGGTFKSKFGDKPISAYAGKAQSAALIAIDVKNGEVLASSSYPNYDP
                     NKFAKGISTEDYKALQPKNPNDLLAGSPLLNLVTQGEFQPGSSFKMVTSMAALENGLD
                     PNFTINDPGVIMLGKKSFGDYVWNHGRGNHGMTNLYKAIQESCNIYMATIGTGKTWPD
                     GKSIGIDMNANKILEYAKLFGLDQNTGLQDEVEERAGKVPSTEDKLKSTQALLKSNLE
                     REMANDFVDITREKNPKEYEKRINEIVSWAAEKKTPGRVETMNRLKKLDVKEDRIEEV
                     ADLAVFSYFNFAKWSTADTFNLAIGQGENAYTPAQISRYVAAIANGGNLVELSVVDRA
                     VSSDYSSVKINDQKKVEKIPFKNPDNLKELTKGMKLVARQGTAKSAFADFPIDVAAKT
                     GTAEKSGKIPTDNEYEYLKSHMSSYNVNLNDAIKLADKMKAEKEKELSLAKEKEIKKK
                     LENKDLKDEERKKLEEELEDGVKVRLEDTDKVNSSYLRKAIKELNPKITDDQIDRFKQ
                     DYGSFTWTVAFAPADDPEIAVVCVIPQGDSSVFSLLPTREVIGTYMGLEPTNSKNDNK
                     TNDVNNSSDENINFESQINR"
     gene            complement(27168..27656)
                     /gene="mreD"
                     /locus_tag="ACGI59_000640"
     CDS             complement(27168..27656)
                     /gene="mreD"
                     /locus_tag="ACGI59_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFG9738447.1"
                     /translation="MKKVLLCLLGIVLMTIENSITNYIDIFGISFNLVLIYVTIISLY
                     LDELEGGIIAAIIGLIKDITVGGIFGVNALILFIIAYAIGYMRDQLYKGSYIITFVLI
                     FIGSLIDSIVNIGTSSIIYQSYNISTLFVKGLLVAPIVNSLIGLVIYRLSKRAVLKLK
                     ED"
     gene            complement(27671..28564)
                     /gene="mreC"
                     /locus_tag="ACGI59_000641"
     CDS             complement(27671..28564)
                     /gene="mreC"
                     /locus_tag="ACGI59_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFG9738448.1"
                     /translation="MALRFDKNKNEKKRINVKVIATGVVAITLIGIVGISIGKFSSGS
                     PVNLGVASDAITSVGKGINDGFSFIKNGFKDVANFKDNSKKVKKLEEENEKLKKNMIA
                     LNAKLDKTESLEELKKTLNFVQEEYKATSISASVVGKNDGNWYESFVIGAGKNSGVKK
                     ESIVMNGSGLVGIVYEVSNNYSKAISLLDSKASVSFKLAKDANAKGTITQNTTLDNKD
                     SYNSKGYLQGYMFDSSYNVIQGDIITTSGLGFFPDGIPIGEVEKVVDDKDKSLKYVVV
                     KPYVDFKNINDVVVIEPRNIG"
     gene            complement(28574..29635)
                     /locus_tag="ACGI59_000642"
     CDS             complement(28574..29635)
                     /locus_tag="ACGI59_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HFG9738449.1"
                     /translation="MAKEKKKEKKGFFSFNKMTKDMGIDLGTANTLVYIKGQGIVVRE
                     PSVVAIRDDSKEVLAVGEEAKKMIGRTPGNIVAIRPMKDGVIADFDITQSMISYFIQK
                     AADKKGVVSPRIAICVPFGVTEVEKRAIEEAARQAGAKDAFLIEEPMAAAIGAGLKVE
                     EPEGNMVVDIGGGTSEIAVISLGGIVTAKSIRIGGDEFDESIVAYVKKEYNLMIGERT
                     AENVKINIGSTFKDDEEINMQIRGRDLISGLPKTIEICSTEVREALKEPVSSIVDAIK
                     STLERTPPELASDIMENGIMLTGGGALLRGLDKLITQETGMSVQIAETPLDCVALGTG
                     KSVEDQEIFEKVLMMNTKN"
     gene            complement(29705..30364)
                     /gene="radC"
                     /locus_tag="ACGI59_000643"
     CDS             complement(29705..30364)
                     /gene="radC"
                     /locus_tag="ACGI59_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFG9738450.1"
                     /translation="MAPEERPREKMLAKGVKSLSNAELLAILLRTGNKNKNAIELANY
                     IINRDIQGIRHLEDMTIEELCNIDGIGLSKSTQIKAALELGSRVASFKPIKYKIRNPW
                     DIQRYYMDSLRYLKKEVFKAVLLNTKNEIISDVDVSIGTLSSSLVHPREVFKEAIRRS
                     ASKIIVMHNHPSGSVEPSREDKNITSRLIKCGEIIGIEIIDHIIIGDGLYFSFKENMI
                     I"
     gene            complement(30409..30993)
                     /locus_tag="ACGI59_000644"
     CDS             complement(30409..30993)
                     /locus_tag="ACGI59_000644"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HFG9738451.1"
                     /translation="MNIILASASPRRKEILENTNVRFDIIKNEIDEIILEGETPKHLV
                     MRLAFEKSMSVASEHNEDVVIGADTVVVLDNAILGKPKDESCARDMLKRLSGREHQVI
                     TGISLINLCEDKKVIDYVISNVKFKTLSEQDIEDYLKTNESFDKAGAYGIQGYGALLV
                     EEIRGDYFNIVGLPISRLGDLLKKYFSINLFYGV"
     gene            complement(31213..32184)
                     /locus_tag="ACGI59_000645"
     CDS             complement(31213..32184)
                     /locus_tag="ACGI59_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HFG9738452.1"
                     /translation="MVILTAVLVLGIMGLIFGIVLDFASKKFAVEVDERVEAILGVLP
                     GANCGGCGFPGCGGLANAIVEGNAPVNGCPVGGADVGAKVGEIMGISAEAGEKQVAKV
                     ICKGTCSSAKDKYEYEGISDCRAANVLNSGAKMCKFGCLGLGTCKDACKFDAISIVDG
                     IAVIDEEKCVNCGKCKEVCPKGIIITKPESQEVVVECNSKEFGKAVKEKCTAGCIGCG
                     MCVKACKFDAIIFEDKIAKIDPNKCVGCMQCVAKCPTKVISGDITKKKKVTIDQELCV
                     GCTVCKKQCKFDAIEGELKEKHKVDADKCVGCHLCMEKCPKKAIKIL"
     gene            complement(32200..32775)
                     /gene="rsxA"
                     /locus_tag="ACGI59_000646"
     CDS             complement(32200..32775)
                     /gene="rsxA"
                     /locus_tag="ACGI59_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HFG9738453.1"
                     /translation="MNLILLFLSIVLVNNVITSQFLGICPFLGVSKKVDTAVGMGVAV
                     TFVLTLASIITYFIQILLVKTGTGFLQTIAFILVIASIVQFVEMVIQKMSPSLYQALG
                     VYLPLITTNCAVLGIALVNVQKSYNLVETIINGFGAGAGFTLAIVIFAGIRERLELAD
                     IPEAFKGFPITLISAGLMSIAFLGFTGLIKL"
     gene            complement(32790..33383)
                     /locus_tag="ACGI59_000647"
     CDS             complement(32790..33383)
                     /locus_tag="ACGI59_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit E"
                     /protein_id="HFG9738454.1"
                     /translation="MNLAKVFKNGLIDENPTFVQVIGMCPTLAVTTSAINGIGMGLST
                     AAVLICANLVISLIRKITPDKIRIPIFIVVIATFVTIVGMLLKAYVPALDKALGIYIP
                     LIVVNCLILARAESFAFKTGAMPSIVDGVGQGLGFTVALTIIGAVRELLGNGSLFGMT
                     LFGASFQPVLIFILPPGAFLTLGFLFAGFNKLRSKKA"
     gene            complement(33396..33965)
                     /locus_tag="ACGI59_000648"
     CDS             complement(33396..33965)
                     /locus_tag="ACGI59_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit G"
                     /protein_id="HFG9738455.1"
                     /translation="MNSMVRLGGTLLAISAIAALALGATNQVTAPVIEQRNIQANNEL
                     RKAVLPEAKEFKEMKEDTYKGLGDGLIAEVYEGLDGSEVVGYTLKAKPSGYGGEIEVM
                     VGISSEGQVTGVDIGNMSETAGLGAKAKDDAFKGQYKGKTAEPLEVAKGSTTADNQIL
                     AISGATITSTAVTTGVNAAIDVFNSALNK"
     gene            complement(33965..34942)
                     /locus_tag="ACGI59_000649"
     CDS             complement(33965..34942)
                     /locus_tag="ACGI59_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HFG9738456.1"
                     /translation="MENKLIVSSSPHVRSNEDTSYIMKQVIIALLPAAVAGVYFFRLN
                     ALSAMFFCILGTVGTEFLYQKFTKQKSTIGDFSAVVTGLLLAFNVPASLPWWMCLVGG
                     IFAILVVKMVFGGIGCNFVNPALAARAFLLASFPVAMTAWTQPGVNWIGKNLDAVTTA
                     TPLSFLKNGAAGLADLSSNGISLADMMIGNIGGCIGETSAILLLLGGVYLMYKGIINY
                     VIPVFYIATVFILTFLLGGFNITFAIYQLFAGGLMLGGFFMLTDYTTSPMTKKGQIIY
                     AVLAGLITTVIRMYGGYPEGVSYSILLVNCLAPLIDKFVRNRVFGEVAK"
     gene            complement(34965..36302)
                     /gene="rsxC"
                     /locus_tag="ACGI59_000650"
     CDS             complement(34965..36302)
                     /gene="rsxC"
                     /locus_tag="ACGI59_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HFG9738457.1"
                     /translation="MKLLTFKGGIHPPYRKEYSNTKALEKAQAPKIVYIPLQQHIGAP
                     AKPIVEVGDEVKFGQKIGEQQGFVSCNVHSSVSGKVIAIEQHEVPGGSAQCVVIENDF
                     KEELHESVQPKGQLEDLSKEDIVGIIKEAGIVGMGGATFPNHVKVSPPPDSKAEVVIL
                     NGAECEPYLTADHRLMVENPEDVVFGLRALMKVLDVKKGFIGIETNKPDAIEAIQNVA
                     KDYSEIEVVGLQVKYPQGAEKQLIYACTGKEVPSGGLPIAAGAVVDNVATAAQIAKSI
                     KTGMPLVERITTITGSCIKEPKNLITKVGTLVSEIIEQCGGFKEDKKIGKVIMGGPMM
                     GIAQYTTEIATNKGSSGILCLDEEESRTPDIQNCLRCGRCTDVCPSFLQPLFISAYSL
                     KDDYDTAEYHRAMDCIECGSCSFICPARRPLLQSIRSAKREIGAKRRKQAAQK"
     gene            complement(36518..37462)
                     /locus_tag="ACGI59_000651"
     CDS             complement(36518..37462)
                     /locus_tag="ACGI59_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9738458.1"
                     /translation="MNRRKTIYRGFNNNRHKIDLRKYVITIACLCLIGYYSYTKIKDS
                     KILEYVSAKIPFLNNSSDITYKDISDELNSIKKGKKSKSQTNSDDKQETNPEKAVNNT
                     KEPEEVKLATIEGWDMYTIQVAAIDNNDDLKKIQTSLVNNDIPFSVMEKDGVKKIQTY
                     SSFDENDVRKQISSVRKVFPDAFLSHLDAPMLSLEYTSNYAYIESISKELNKLITNFK
                     EESSFWSNAENNVDMEKYNTILTNRKAISQNISKEAEKIDYSEMRLFKDNLIEYVKNV
                     NEKIDTASKAANEEKYSVSKSLLLSSMQEYSMFINSIK"
     gene            complement(37573..38847)
                     /locus_tag="ACGI59_000652"
     CDS             complement(37573..38847)
                     /locus_tag="ACGI59_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HFG9738459.1"
                     /translation="MKKAIAALGIGAVAVSVSSINASALEKGTVTASALNIRSGPSSD
                     CDKVAKLYKGKTVEILEKSNGWYKVRVSSSVVGWGSAKYISTSGSSEGTSSQNNPTSS
                     GTTISGNGKVNVSSRLNVRSGAGTNYSLVGKANNGDVVKLLEQSNGWYKIKLSNGVTG
                     WASSQYISKTSEDVGTNNSSNSNSTNNSDKKPSSEESIEGKNGKVTSAVSLNVRSGPG
                     TSYSIIGKLNGGDVVELKAKSNGWYKVKLSSGTIGWVSASYISETNEDTKEKPNSSSN
                     QNSQSNSNSKPSFTGNSDKSTAKGSTIVDFAYTLIGIPYQWGASGPDKFDCSGFTQYV
                     FKHSVGVSIPRVSREQANFGSAISMGNYAPGDLVYFDTDGDGTTNHVGIYVGNSKFIH
                     CSGTQTNPNKVKVDNLTSSYWSKVLLGARRFV"
     gene            complement(38979..39350)
                     /locus_tag="ACGI59_000653"
     CDS             complement(38979..39350)
                     /locus_tag="ACGI59_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFG9738460.1"
                     /translation="MKFNFCHNNFNVTNLEKSLDFYKKALGLKEVKRKEAEDGSFILV
                     YLGDGITSHTLELTWLRDWDRPYNLGDNEFHLALEVEEFDEAKKLHKDLDCICFENES
                     MGIYFIADPDNYWIEILPKNH"
     gene            complement(39578..40177)
                     /locus_tag="ACGI59_000654"
     CDS             complement(39578..40177)
                     /locus_tag="ACGI59_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9738461.1"
                     /translation="MESRGYKEDLKKMILDMAHKELESYVSQKGAKKYFEGNLDKIIN
                     NNIKKFNQSIGLNRVYLKMLKKCAYQESVTSISRTIKKEALFKSKKELFAFSEYLKLD
                     INKRLSYNQILRKISRYIYYNRSSYAQKYVVFKRGDEEYLLEPEKIKDELISSYRSKT
                     REDMRSIAKLLDIEVEDDYNAEDIRKKVINYIIKEKLKS"
     gene            complement(40378..40590)
                     /locus_tag="ACGI59_000655"
     CDS             complement(40378..40590)
                     /locus_tag="ACGI59_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HFG9738462.1"
                     /translation="MKKKLLVEGMSCGHCVNHLKTALTEDIDGIEVLDVDLENKCASV
                     DMKDDVSIEKLKEVIAELGFELKGIE"
     gene            complement(40663..42378)
                     /locus_tag="ACGI59_000656"
     CDS             complement(40663..42378)
                     /locus_tag="ACGI59_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFG9738463.1"
                     /translation="MKRIKVLTVVLAITLMVAGCSNTKTKQSSSDSSLQSQSSIDAKY
                     INLTMVTPKTINPITNTDKSVGYIMNLVYDSLFTIDENYNVIPQLVKEYNIAQDGMSI
                     DIKLKDAKWHDGKNVTSNDVNYTIGLIQKSADSPYNEFTKNIASVNIKSDKDFTIKFK
                     ARYAFSIDSLIFPIVSQNHLDSKDVNDKNNNMIGNGKYKIESYTEREGMVLSVNKDYY
                     EEVPKTMKNIKVGMVPNEDARTSMVMALDSDITNVTLNDLSKFQEKEFNITKYQGRDY
                     ECVLFNYNNPFFKDVNFRKAIAHSIDKDRIISEGYMDDATPVNFPLNSKSKYYNSEMK
                     DLEFNKDKAIECLAKVEYANVNSVNQNDNKVKNQKKSAKKNSKKLTPEEEAKVKKAEE
                     DRIKKEAEEKKNREKEEVKKSLSEMNLKIIVNRDNSERIKTANIINENLKAIGINSTV
                     NQLSDKDMENALNSKNYDLALVGWKLSIIPDASSIIASSGYTDDKLNGYMSALTSSTS
                     EIETKKAYKDVQTYIKDNATFISLAVRNNYIVSNSRLKGKITPNDFDVYEGISNLDIK
                     SNESN"
     gene            complement(42378..42932)
                     /locus_tag="ACGI59_000657"
     CDS             complement(42378..42932)
                     /locus_tag="ACGI59_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFG9738464.1"
                     /translation="MNSKKVLILSIFIILFGALLMESKVIHKFLYPKKYSEYVEKYSK
                     EFNLDENIVYSVIKAESKFNSSAVSKKEAKGLMQILDITRDWGAEELNLKNVDIFDPE
                     TNIRLGCWYLSKLYKEFGKLDLVIAAYNGGSGNVKKWLENNEYSKDGENLHDIPFKQT
                     SKYVEKVKNNYEHYNKIYGKKGKN"
     gene            complement(42925..43527)
                     /gene="coaE"
                     /locus_tag="ACGI59_000658"
     CDS             complement(42925..43527)
                     /gene="coaE"
                     /locus_tag="ACGI59_000658"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419019.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFG9738465.1"
                     /translation="MLILGLTGGIGCGKSSLSNIFRNLNIPIVDADIISRKIFEDKLL
                     LEKVFVHFGQSIKNDDGTLNRKALGKIVFSDEEKLKELNNLTHPRIREKIISEIEKLR
                     KKGENIVVLDAAILVESGFLDMVDKLLVVTCKQEVQISRIQKRDNCSEQEALSRINSQ
                     MSQEEKSKYGDYIIDNSGTITELESKAHKFIEYMKENWRE"
     gene            complement(43536..46184)
                     /gene="polA"
                     /locus_tag="ACGI59_000659"
     CDS             complement(43536..46184)
                     /gene="polA"
                     /locus_tag="ACGI59_000659"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFG9738466.1"
                     /translation="MEKKLIIIDGNSIINRAFYALPEMNNKEGLKTNAIYGFTTMLFK
                     MIDIYKPTHISVAFDRKAPTFRHLEYKEYKAGRKGMPDELAEQLQPLKDLLDKFRINR
                     LEIDGYEADDIIGTVSKKAEENGYKVYIVTGDKDAIQLASDNTTTLITKKGVGEVEEY
                     NFNSVIEKYEMTPTQFIDLKGLMGDKSDNIPGVPGIGEKTGIKLIKEFSSIENLIENT
                     DKLKGSVKKKIEENKEIAIQSKRLATIIRDVPIEVDLDSMIFGDYDKDELLDKFRYFG
                     FTSLLSRLVDLVDSDDTEHVEEKIEILKLKDIEKFIDEVNKKGQVILKTVRGQGNILE
                     KNIIYIFISVDGEKIYYVDSNEVSKLDSIFSNNEIKKYGYNLKDDYISLKPYNINLEN
                     MYFDITIAEYLIDSTSSTSYDCSAIAMKYLSKKVKSKEELLGKGVKSKNYEDLEFEDL
                     AEYMGQIICTVKETVPMMERSLMDMSMDGLFYHVEMPLVEVLGHMEYEGIKVDKSILD
                     ELSVEFKEIIATLEKEIYELSGEPFNINSPKQLGVILFEKLELPIIKKTKTGYSTNAD
                     VLEKLRDKHPIIDKITEYRQIVKLNSTYVEGLLNIINPESNRIHSSFNQTITTTGRIS
                     STEPNLQNIPIKMEMGRKIRKVFIADDNCKLVDADYSQVELRVLAHMSQDENMIEAFK
                     HHEDIHTKTASQVFNVPMEDVTSELRGAAKAVNFGIIYGKSDFGLADDLNIPVPKAKE
                     YIESYFAKYHKIKEFMDTTVEKASEDGYAVTILNRRRYIPEIKSSNFMERNRGKRFAM
                     NAPIQGSAADIIKIAMINVHKKLEENNLKSKLILQVHDELIVEAIDDEIDIVKKIVKE
                     EMENAVNMDVHLDVDLNVGSSWYETK"
     gene            46336..46423
                     /locus_tag="ACGI59_000660"
     tRNA            46336..46423
                     /locus_tag="ACGI59_000660"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:46370..46372,aa:Leu,seq:caa)
     gene            complement(46610..47053)
                     /locus_tag="ACGI59_000661"
     CDS             complement(46610..47053)
                     /locus_tag="ACGI59_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9738467.1"
                     /translation="MKKNMLYVGIIYFICGILLVLLATFTEFSFEAFIWGLSGAALGP
                     GVCMILQYMYWSKPERAVDYEEKIKNQRIEMNDERRIMLRDKAGRITNQIMSYVLVIL
                     IFIVSILSVFSVMVISKWVLVVLGLLILFQFICSVVVYNKLDKKL"
     gene            complement(47056..47253)
                     /locus_tag="ACGI59_000662"
     CDS             complement(47056..47253)
                     /locus_tag="ACGI59_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004631111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFG9738468.1"
                     /translation="MKNRLEEIRKERGIKQEELASILQVSRQTIGSLENGRYNPSIIL
                     AFKIARYFDMSIEEIFIYEEE"
     gene            complement(47445..48311)
                     /locus_tag="ACGI59_000663"
     CDS             complement(47445..48311)
                     /locus_tag="ACGI59_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFG9738469.1"
                     /translation="MEWINKLNQAITYIEENLESGVDYAEAAKIACCSTFHFQRMFSY
                     IAEVPLSEYIRRRRMTKAAFELQNSNIKILELSEKYGYDSPTSFNRAFQNIHNISPSA
                     ARAKGVVLKAYPKMTLSIYVKGNIEMQYRIVEKKGFRIVGIKESMNMIVEECFEKVPK
                     LWAKCIKNGTIDKLSELINNEPCGLLGVSVCTNSKGLDYYIAAPTDKPVLEDTHEYLI
                     PSGTWAVFECVSPMPNALAIQELQKRIITEWLPSSGYVYANLPDIELYPNGDINAPDY
                     TTEVWIPIQKPS"
     gene            complement(48537..49406)
                     /locus_tag="ACGI59_000664"
     CDS             complement(48537..49406)
                     /locus_tag="ACGI59_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFG9738470.1"
                     /translation="MDYKTIILNRKSVREYKNTDIKEEYLSEIKKYSESCKKLVPEID
                     VDVRIMNRSEVFNNLDKVAGYKGNMIDSPSYIIILSDIKDNYIENSGYIGESIIFKVT
                     ELGIGSCWVTFEDGKKVKEKLGINSDKEVTGIIAIGYGENVSNAKVLNATKTGQNYSK
                     SDMQVVTDKGSTRLGVEDVVYMNEWGNVASIEILEERALLDAFHYARLAPSTLNRQPW
                     RFIVDGGTVVLAVRKDGHTNLYEEKIDIGIVMLYFATIISATMFDLKWNLGTPDKDYK
                     VPEDYKIVGYCNI"
     gene            complement(49912..50133)
                     /locus_tag="ACGI59_000665"
     CDS             complement(49912..50133)
                     /locus_tag="ACGI59_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9738471.1"
                     /translation="MNVNNHKCPECGEHSKKFEIVEENEDCVIFECYTCGTISILKYC
                     ENCKEFVFVNIPNNVETYEYCCGKCNNKI"
BASE COUNT        15565 a         9444 c         5474 g        19848 t
ORIGIN      
        1 tatattttcc ctggatttaa aatattttta gggtcaaaag ctaactttat atttttaatt
       61 aacatcatat aagcatctga atttgattca tgtaagtact cttttttagc atatcctatg
      121 ccatgttcac cagaaacttg accactaagt tctctagatt ttttatacat atagtcaaaa
      181 gtttctttta atcttatttt ccatgtatta tcatccatac catcttttaa aatatatatg
      241 tgtagatttc catctcctgc atgaccaaaa ctttttattc ttatttttaa tttgtcttga
      301 agctcgtgag tgtatcttat aaactctgct atcttatctc ttggaacaac gacatcacat
      361 tcatccattt gtgtcgtaga tgcctttata gcttctaaaa atgcacctct ggctgaccat
      421 atagaatcgt ttctttcttg tgtatctgat ataaatacat ctaatgcacc attttctaaa
      481 cataaattag ctactttttc atattctttt tctatatctt cagtgctatt tccatcaaat
      541 gtaagcaaaa gataagcatc tgatgtatta tctggaaact ttttacctaa aaattcttct
      601 gctgccaata tcacatctct ttccataaat tctatagccg ttggtattga ttttgatttt
      661 attattttag gaacagtttc tattgccatt gataaatctg gaaatggtat aagcaaactt
      721 atacttttct ttggtaaagg aagcaatttt aatatagctt tagtaactat tcctaaagta
      781 ccttctgagc ccactaataa atcctttata ctatatccag aactattttt aaccaccttt
      841 ccacctacat ttataatttc tccatttggt agtacaactt caagtcctct tacataatcc
      901 cttgtaacgc catattttac tgctctcata ccacctgcat tagtgtttat attacctgca
      961 attgtagcac ttttttcacc tgggtcaggt gggtaaaata aatctctatc ttgaacatat
     1021 tgacctattg tcatcaataa aacacctggt tctactgtca atgttaaatt ttcataatca
     1081 acttcaagta ttttattcat tttacataga ttaatcatta ttccaccatt tatggcaaca
     1141 gcagcaccta caagaccagt accttgacct cttggtgtga ctggtatatt atttttatat
     1201 gcgtatttca ttattttaga tacttgttca gtttcaagta catttactaa aacttcaggg
     1261 tacttctcaa tccctccaag ttcatcatgt gaaaaatctt catttatatc acttccaact
     1321 aatacatttt cttctccaca agtatctatt aaaaaatcta tatctttttt atcgattaat
     1381 ttatacattt ctatccccct atttagctgc tgatgccata tatttttgat tattatcttt
     1441 gttattttct attttttcaa tcaatttagg aataatatca taaatatcac ctattaaagc
     1501 taaatgtgct acatcaagta taggcgcttt atcatcttta tttattgcta ctatatagtc
     1561 agcaccctgc atccctgcaa caaattgaac tgctccagat acaccacaag ttattatcaa
     1621 tttaggtttt acagttctac cactaagacc tatttgtttt ttagcatcta tccaacctgc
     1681 ttctattaca ggtcttgttc ctgccacttg agcgtttaac ttatcagcta atttatatat
     1741 catctccata tcttcttgtt tttttattgc tctacttgcc acaactatca cttcagcttc
     1801 ttccagacca acttctttat ttttttcttt tacactcagt accttaattt tagattttag
     1861 ttcttttttt tctactttac ataaagttat ctttcctgta gtattctcta ttttttctgg
     1921 tgctgaaaat attttatatc ttacagtggc aaattgtggt ctattattag gtgtgtttat
     1981 atgagccatt atatttccac caaatgctgg tcttatttgg tctaaatcag tatttgattg
     2041 aatatcaagt attgtacaat ctgctgtaag acctgttcta aatctagcgg caagtcttgg
     2101 agctaatgac ctacctaatg tagttccacc aactaatact atcgttggtt ttattttatc
     2161 tataaagttt tctattgctt ttgaatatgg ttctatccta aaatctttaa attcatcttc
     2221 atcatataca aatacttcat ccaccccata agaaatcaaa ttactgcact tatcttttat
     2281 gtcttttcct acaaatacac aatacacagg ttgttttatt tttgatgcca tctcacgagc
     2341 tttacctata agctcgtacg ttacaggatg tatatttcca ttatgatgtt caacatatac
     2401 tgcaattccc ctccattcat ctttattaat ttgtacttta ctactctcta taaattcaaa
     2461 tgcgcctttt ggtccacttt tgacacacaa cttgcacatc ttacagccag cacttatttc
     2521 tactatacct gacttttctt ctatagcccc aaaaggacat atttctaaaa ttctctttaa
     2581 gtcagttatt ttatcttgat ttacaactat ctttgacatt tattactccc ccttacacca
     2641 actttaattc ctctaattta tttacaagtg caaaacttag ttcatctgca tttcctctta
     2701 caatattttt atctgtattt ggctttggag gaaatattct tacaacttga gtaggagaac
     2761 cattaagtcc atagttattt tcatctttat cttcaaagtc atctaagcta tatacaggta
     2821 tttctctatc tttagtatta agttttaatt taaatgaagg tagtcttggt tgaaaaatgt
     2881 ctttttctac agttattaaa catggatatt caatctcaca tacttgtaaa tcatttggca
     2941 tatctatttc aacaactata ctttcatctt taacttcaat taattttgta acgttagtaa
     3001 catgaggtat atctaaaaat tcagctactt caggtcccac ctgtgctgta tcaccatcag
     3061 tagtttgttt tccacaaatt attaggtcta cctttcccat cttcttaata ccttgtgata
     3121 tggtatagga agtagccaat acatctgctc ctccaaatct cctatcactt attaatgctc
     3181 cttcatcagc tcccatcata aaactttctt caattacctt ctttgcctga ggaggaccca
     3241 tgctaagtac ttttagattc gctttcttat cttcctttat tctaagagct gtttctaaag
     3301 catataggtc atagggattc atttttgaat ctactccatc tctttttagt actcctgtag
     3361 tttcatcaac ttctactgaa gtcgttccag gaacttgttt aatacatgtc aaaatttcca
     3421 taaagtacac tcctttttta tttaactata actgatactc catcaggtat aactactact
     3481 ttgctctcct taccaactat actattcgcc atttctaaag cttctttaaa gttaaatgca
     3541 tgtttcatat gcattttttt tatcatttca ggattacact tatctgttac cattatcaca
     3601 gtatatttag ataatattct cgctagtatc tgaaattccc attggtctgg aatcgtatct
     3661 actctatcga cacttctaat tttatctaaa agttgttttg gagaatctgc atttgctaca
     3721 ttttcataaa aagattgacc tccatgacca tcattacatg ctgaaatcat tattattact
     3781 ccaccgtctt tacaagtagc ttcagctgct gtcattcctt taacagattg ataaatattt
     3841 tggtctagtg gatatccacc atttgtagtt acaacaatat cagattttat agaatttact
     3901 tttgataact ctgttacaaa tctacatcct ttagtatgtg ctttttcgac atcaccagca
     3961 aaggaagcaa ttattttttt atctttatct attacaacat tcaatatgaa tgctaatttt
     4021 gcaactttag ctgcatatac catatcctca tgaataggat tattattcaa ttttccagtt
     4081 cttgaattat cactatctat aaagtcacta caatgattgt acattatagt ctttgcagat
     4141 gcgattcctg gaagtacact ttttctgcca cctgaaaaac ctgcaaagaa atgaggttct
     4201 ataaaacctt ctgctatcaa aagttctgcc tcatatgcta gtttgtttat atataaatct
     4261 ccacctgaag gcaatatccc tactttacac atagatttat catcagtaga tatatgcata
     4321 actatatctt cattatctac tatttcttct cccattttat aaattagctc ttcccttgta
     4381 cttggcctat gaaatccagt tgccactata atcttcacat caatttctgg attaacattc
     4441 ctaatccttc ttaatataat tggcatagtt attctacttg gtacaggtct tgtatggtca
     4501 cttgtaatta tgactacatt ctttttcccc ttaaccagtt cttccagaga attagagcct
     4561 ataggattat tcatagattc ttcaactatc tcttcttgag tcttatgttc tatataatta
     4621 tccgattttg actccaaaac accgagaaaa cgttcatcag gtatttctaa aatcattcct
     4681 tgttttgaat acggtacttt taactttacc atttttaatc tcccattata ataaactata
     4741 aaattggtat gtggtcatac cagttttaca tttatatgat agtaccatta tctaccattt
     4801 gcaagaataa tttattttat aacaaacttt taattttcat tttttctata atttttactt
     4861 ataaaatcca tatgttcctt tgctttctca catgcaagtt ctacatcatt ttctacaact
     4921 gcattgtata tagcttcatg ctgtttaagc aatatatttt cattaaaagg ttctgcgatt
     4981 attttttctc ttgcatcttc tataaatcca tcaaataatt ttgatgctgt cataaaaaga
     5041 ctttgaatta aataattttt acttaatgtt gctagtttac tgtgaaattt ttggtctaat
     5101 aacaccagta ctcgattatt atctttttta ttagtttcta ccctcatttc atctattata
     5161 tgtttcaatt ctataacttc agaagaagtt attctctttg aagcaagttt tgcaatctca
     5221 atttctagga ttattctaag ctctaatata tcttcaagtg taccattatt aagtttaaaa
     5281 atcatagata aaggttctat taaagttttt tctatatttg tacatataaa atttccttca
     5341 ccctgtctgc tttctatcac tcccatagtc tctaataccc taatagcttc ccttatggaa
     5401 gttctactca cattcatttg ttcagacagt tctctttctg atagtaattt atcccccttt
     5461 tttaactctc catttagtat tttatattgt atttgctcaa ttacttgttc ataaactttt
     5521 ttactagcta tactattaaa cattttttcc tccaatatga taatcgttgt tgtaatatat
     5581 atctattata tatcttaagc cattacattc atatattttt attactttta atataatatc
     5641 ttataactca aatttacagg tgatattatg aaaaaaaata aaattattgc tttttttgct
     5701 ccatctatca tagttggagt tctagtttta gggattatcc tatacccaaa tgaagctata
     5761 aaatctgcaa gagaaggctt ttctatatgg tctaatgttt tagttccatc actactacca
     5821 tttataattg gagcaaactt aattgttgat ttaaaaatag tggatattat agggtttatc
     5881 atcaatccca taactagatt tgtatttaat gtttcaggaa agagcgcttt agcttttgct
     5941 atatctacag tatctggtta tccagtagga gtaagtttag cttctgaatt taggagtaat
     6001 ggacaaattt ctaagtatga agcacaaaga cttgtgtctt tttgttctac atcaggacct
     6061 ttattcataa taggttcggt tggtacaggt atgttccaaa attcaaactt aggttattta
     6121 atgattctct gccattatct aggaactata ttggttggtc ttttctttag aaattatggt
     6181 aatgaaaatc ttccaaagac aaaattggat ttaaaaacaa atataaaaaa tgtaatcaac
     6241 attagaaatt caagtgggaa tggttttttt gttttatttg gaaatgctgt atttaatgga
     6301 gtaaatacat tacttacagt tggtggattt gtaattgttt tttcagtagt atttaaaata
     6361 ctatctcttt ttaatgtaat ctcattaata gcatcagtta tatatttgcc tctttcattt
     6421 ttaggtgtta gtaaagaact ctgtcaagca tttgtaagtg gtctatttga aattactata
     6481 ggatgcaata aagtatctag tataacaagt tctccagaaa ttttaagagc atctttagct
     6541 agttttttga taggatttag tggattatcc atcctagctc aatgttgtac atttctatca
     6601 aaaacggaca tagatttaaa attatacata ctaagtaaat ttgcgcatgg agttttagct
     6661 gctatcttta tatttatact ttatccaatt gcaaactctg ctgtgttggt ttctagcttt
     6721 gctgatacat acaacgttat atataataat ttaatctggt tttattatct tagctattat
     6781 gatacaattc ttcaaattgt agtaataatt tatctaatct cagccgtatt tattgcaaaa
     6841 aaacatatga aaactttatc tacaaatggg aaactaatgc gatataaaaa tacttttttc
     6901 actaagaaat tttcacgaaa aaaataaaac taaaaataaa actaataaaa cttaactaaa
     6961 tacttccatt tttttattat atttgtcata attttaataa cctttttaat tagtttaaca
     7021 gcatagttat ttcatcacaa tttaataatt ttattagaaa tatttttcaa aaaattctcc
     7081 ttaatcacat atatcacact tgtataagtt tactgggaaa tttttccatt atataatatt
     7141 tcatttatat tatataataa ataccgacct aaacttacca aatcaaacta agaggagtgg
     7201 ttgaaaatgg caacaagacc tatagaaata gacgaatata aaaagataat ggaactatta
     7261 catactggct ttacttatag tgaaaatggc gttgaaaaaa gatttagaaa aaatcctaaa
     7321 gttgcattgg cactaatgtt agaagctaat ttaggtctaa gaatatcaga tattttaaga
     7381 ttaaagatag gtaattttaa aggtaatatg ttggaaataa atgaaaaaaa gacgggcaaa
     7441 ttacagtata gaccagttaa taaaaatata attgagtcca taaatgaaca tgcaagaaag
     7501 tataatctta aatcaaatga ctacttagta aatataaaga caaaggctat tcaaaaacag
     7561 ttgaggataa tttgtaaata cttaaatctg tataatataa gtacacatag ctttagaaag
     7621 ctctatgcta ctactcaatt tgaaaagagt aacaataatt tagagttggt taaagaactt
     7681 ttaaatcaca gctctgtagc cactacacag cgatatataa gggtaactca gcaagctatt
     7741 aatgaagcta gtgaaaattt ctttattggt taattataac gtatacattt ttctattatc
     7801 ttattaattc accagatatt aaaaaagagt gtctcaaaat gaacttttta gttcatgaga
     7861 cactcttttt ttataaaatc aaatatattt ttattatttc aacactggaa aaaaggctta
     7921 tctctctatt ttgagacacc ctattaaatt ttcataattc ttgcataaac atattaatct
     7981 atcttcaata attttttagc tgattcactg taattatttc tttttacaat ttccaaaact
     8041 ctatcaatct catgttctga ctttgcactt acaaaaatcg ccaattcttc atcatctata
     8101 atatcataac tatctacata tttatctaaa cattctaaaa ttccatcttt attattatac
     8161 ccaccaaatc taactactgc actacactca tcttctataa gtccacttaa atccaatttc
     8221 ccttttgaaa aagactttat atttgaactt ctattatcta ttatatctat aatatctgca
     8281 taaacagcac ttcctgttgc acctcttcca gctcccttac ctacaaaaga aagttcacct
     8341 actgaatcac ctactaaaat aactacgttg aactcattat ctatctttga aagcatatta
     8401 ttattgtcca ctaatgctgg tctaacaaat ccacttactt ctcctgattc atacttactt
     8461 ctagcaacca attttatctt acaatttaat ttttttgcat attctatatc tttttcatct
     8521 attgaagtta ttccttctaa taataaatct ttccaatata ctatttttcc ataagataaa
     8581 gtagacaata tagataactt tctagcacta tcatatccta aaacatctgc atctggatta
     8641 acttctgcaa atcccaactc ttgtgcctgt tttaaagcga catcatacgg taaattttca
     8701 tcatacatct ttgataatat aaaattacaa gtcccattta aaatagcgtt tatgctcttt
     8761 atattgttgc cttccagtga ttctatcaat ggttttaaaa ctggaatacc acctgccaca
     8821 gatgcttcaa acttgatgct aacattattt aagttagcta attctataag gtcactacct
     8881 ttttcagcca gtaaatcttt attagctgtc actacatgta tttttttatt taacgctctt
     8941 gttacataat caaaagctgg atttaatcct ccaattactt caactagtat atcaatatcc
     9001 ttgtcaaata tatcttctat atcagtagtt atcttatcaa aatacttctt atacttgtgt
     9061 ttttctttat ttttaactaa aattccaaca acttctatat ctctttttga agtattattt
     9121 tctattaaat caattaatcc acttcctact gttccatacc ctaatattgc tatcttcact
     9181 gtaacatacc cccatcattt tgattatatt aaattattta tatatataaa tataccaata
     9241 aaaaaagctt gagtataaac tcaagccata tgacgggttt atactcatct ttcagcataa
     9301 atgctgcagg atttagcacc aatgtataat taaatatact ggttgccggg cttcacaggg
     9361 ccagtccctc tgccactctt gataagattg ctaatttaaa tttatgttta atagtataat
     9421 acaatttctt ccaattgtca ctattttttt aacttatttc cctaaaagga tataaactat
     9481 tatctaacag ctcttttagt tggtttaccc ttttttgtaa cattattatt tttagtcttt
     9541 gctttcgcct gttcatgtcc tggaaaaagt attttaggct cttcaatatt cctcttgtaa
     9601 atagtaaatt taaaaccaat agcctgcaca aattcagctc ttaaagcttc acatactgca
     9661 ttagcagttt ctttagtatc aagacctgca ttttctaata tagtaacttt tactaattct
     9721 ctagttctta acataccatc taattgctct aagaaactct ctgtaacacc ttctttacca
     9781 atttgtgtag taggctttaa agtatttgcc aaagatctta aatacgccct ttgttttcct
     9841 tttaacatat tctcacctct ataattctat acttaaatta tctataatct atatttaaat
     9901 taattgtaaa actcaaattc taattcataa attttaacca catctccatc ttctattccc
     9961 atttctctaa gcctatcaaa aacaccttga ctctccatag ctttttggaa atattgaagg
    10021 gattccatat catcaaaatt tacagaatac attattcttc taagtgcttt acctgtaact
    10081 acatacacac catcttcaat atctatactt aactcttctt cagtaccaat atcaagttct
    10141 ggtctataca tttcttcttc tgaaactagc tccacatctt ctacttcttt tagcatttta
    10201 gaaacatagg ctattacttc atctatgcct tgacgagttg ctgctgacat tttaaatact
    10261 gtatatcctc tgccttctaa ttcactctta aacttctcaa acttactctc atcttctaat
    10321 atatcaaatt tatttgcaac tacaacttgt ggtctctttg aaagcttttc attgtacaat
    10381 ttaagctcat catttatctt gtcaaaatct tcaatagggt ctcttccctc taatccagat
    10441 atatctacaa tatgtatcaa tacctttgtt ctctcaacat gtcttaagaa ctcatgacct
    10501 aatcctatgc cttcagaagc accctctata attccaggta tatctgcaag tacaaaacta
    10561 tctccaaact tagtttgaac aacacctaga tttggtgtaa gtgttgtaaa atgatagttt
    10621 gcaattttgg gtttagcttt agttacaact gatagaaatg ttgatttacc tacatttgga
    10681 aatcctaaaa gaccaacatc tgctatcatt ttaagttcaa gagttatcca tctttcttct
    10741 ccatctgtac cagattttgc aaaagctgga gcttgtctta ctgcatttgc aaagtgttgg
    10801 ttacctttac cacctatacc accttttgca actatagctg tatctccttc tttttttaaa
    10861 tctgctaata caagacctgt agcctcatct cttattactg tacctgctgg tacttttaat
    10921 actaaatttt ctccattttt accagctctt tttcctttgc ttccgtcgcc accattttga
    10981 gcttgatatt ttttctggta cttaaagtcc attagagttc taagccccaa atcaacttca
    11041 aatatgatgc ttgctccacg accaccatct ccaccgtctg gaccaccagc tggaacgtat
    11101 ttttctcttc taaatgctac cgaaccattt ccaccatttc cagccttaac aaatattctt
    11161 gccttatcta taaacaaatt cttcacctac ttaattaaaa acttttaatt agcattacca
    11221 aaaataccat tataaattca tcagtattat aagaaaaagg agtgcctcaa cactcctttt
    11281 atgtatatta ctcagctatt gctactggat atatactaac tttttttctc ttcttgtctt
    11341 ttctttcgaa tttaacagta ccatcaacta atgcaaataa agtatcatca ctaccttttc
    11401 caacattagt tcctggatgt attttagttc ctctttgtct aactattata cttccagcag
    11461 ttactaattg accatcgaat ctcttaacac ctaatctttt agatatagaa tctctaccat
    11521 ttttagaaga acctacccct tttttagatg ctaataattg taaattcatg tttaacatat
    11581 ggagtcaccc cctacttttt taaaattttt atattttttg ggtattcccg tttaatttct
    11641 ttaaccgcta attgaaagtg atttagtaat aattgtgcgt cttttacatg tgtatctttg
    11701 actatacact ctatatgacc ttcttctcct actacttttt caaattcaat cttctgtaat
    11761 ttgtctaaag aatttataac tgcaatagca ttagatgtaa ctgcagcaca tactatatca
    11821 tagcctattt cagcaaaatc agcatgtccc tttaaacaaa aaccttgcaa caagaaatca
    11881 tcattataat aatatttaac ttttatcata acttaaccaa taactaagcg tttatttttt
    11941 ctacaactat cttagtgtat gattgacgat gtccttgttt tcttctgtaa tctttttttg
    12001 ctttatactt gaaaactata actttcttag ctttaccttg ttcaacaact ttagcttgaa
    12061 cagatgctcc ttctactact ggagaaccta attttaattc tccatcttta gagcaagcta
    12121 atacttcgtt taaagtaaca acatcacctg cgttagcttc taatttttca acgaataata
    12181 catcaccttc agaaacttta tattgctttc caccagtctt tactatagcg tacattaaat
    12241 acacctcctc ggcctcaaac tcgccagtac aaggtacttc atttgaagat ttaaaccttt
    12301 actgtgcggc attacaatta ttaattataa aatatttaca ttttattgtc aactatttta
    12361 tcgaaaataa cattggtttt attgtgcttt aatgtactat ttgcgtttaa agaaatcttt
    12421 atattatatt tttcaccaat tttatcaatt atatccatat aatattcttt tatttgttca
    12481 aaaataaaat cattaaattc taccattatg tttttgtaaa cagtatgttc acttattctc
    12541 ataatttctt tttcaatact atctagtata taatgtacag attttattgt ttgttcacca
    12601 tcacaacatg gacaagacat taaataatac ttatcaatgg attctttttc tctacgccta
    12661 gcaacttcta ctaaaccaag ttttgtcata cctaatactt cagcttttct tttatcttta
    12721 ttaaattctt cttctaggat gtgtaaaatc tccttttgat gcttcttttt atgcatatca
    12781 ataaaatcga ttattattat cccagctatg tctctaattc taagctgtct tacaatttct
    12841 tttgcagctt caagattagt tttataaacg gtttcatcaa gtttaccagt tccaataaac
    12901 ttacccgtat ttacatctat aaccgtaaga gcttctgttt tttcaattat taaataacct
    12961 ccactcttta accatacttt tttatttaat aatttttcta ttttagattc tattctgtat
    13021 aaatcaaata catctctatt ttcctcaaga cacagtttat caacataagt cttatctata
    13081 cctcttaaaa tagacttaag ttcagagtac ttatcataac tattggtaat tatagattca
    13141 atatcatcat tcacattatc ttttacatat ttagttgcaa aatctaaaga cttatataat
    13201 aatttcggac ctataccaag tttatattct tttaaaatgt tttcatactt taatttcaac
    13261 tcttctatat ctttctttat ttcatcatga gtgcaacctt gagcctcagt ccttattatt
    13321 aatcctagat tttccttatt tactgcctta gtaatttttt ttaatctaaa tcgttctttt
    13381 tcatcagtaa ttttatttga tattgtagtt ctatcattgg aaggtatata gacaatatac
    13441 cttccagaaa tactaatctc agtgtttaat tttgcgcctt tagttcctat ttcttcttta
    13501 tttatctgaa ccaaaatatc ctgaccagat tttatagtac ttcccttttt taattgtaaa
    13561 taagcaagtt tcttaaatcc aatatctaca aaacaagctt ctattcctgg tataactttt
    13621 tttacgattc cacgatatat attggataca gtcttcttat ttaaattgtc ttcaacaaaa
    13681 agttctgtta atctttcatc ttcaagtact gctgttttct gggaccctat tagggactca
    13741 ataactatct ttttcacaac ttcacctctc gagcttacat aggagtcact aattccccat
    13801 cttctaatac atataaatct cttctcaata tatcaacatc taatgggtca atatcaagtt
    13861 caaacatctc taatatcttt ggtataagta tattagtatt taaattagtt tttgagcctg
    13921 tagctatagt agcagtaaga gtaatatgtt catcttctaa atttaaaaca tcaaaggttc
    13981 ttatcatagg tcttatatct acttcaacca tcttaccttt tttattcttt tttgttatga
    14041 ttatttcttt attattcata aagtcaatta cttttctttt tacaaactct ttcgtcatag
    14101 gtcttttcaa atctatatta aacaaatatt ctccatagtc tatcacagaa gaaagagaag
    14161 gtgtttgtct atcaatttct tttgctttga taaaatctat cccatctgga agttgaattg
    14221 atactttatt taaaaattcc tctactgata aatcatcttc ttctatttca atatctacat
    14281 actcaccttg actttctgcc cctaaagcca aagcattgcc ataactcatt ttagggtgag
    14341 gattaaatcc ttgagaatgt gataagttta tttcagctct tctaaacgct ctttgtaaca
    14401 atctctgaag gtcaagatgt gatatatata tcatgtcgcc ttccttttta aattttactc
    14461 ttattatctt actcatatta gcaaagcccc cttcctatat catgtatatt tattccacaa
    14521 ccattgcagt tatgtctaca atcagaagtg attttttctt ttttagcatt ttcgtcttct
    14581 ctaattaaga attttttact aatacctaca tctatatggt cccaaggaaa tacctcttca
    14641 tagtttctat ttctatgtgc ataaaaatct atagaaagat tattcttttc catagcttct
    14701 ttccatatat ctaaattaaa atgttctgcc catccatcaa atttagcacc tagtctaaat
    14761 gcatcatata taacttttcc tattcttcta tcgcctctag ctactactgc ttccatcaaa
    14821 ctagtctttg aatcatgata attgtatttt atattgttat ttcttaattt atttactaaa
    14881 tgtctctgct tattaataac ttcttctgta gtatcttgac catgccattg gaaaggtgta
    14941 aatggctttg gtacaaatgt agaagtactc actgtaacac taaaacttct ctttagcttt
    15001 cctccattta cttttctata aatatcaata actttataag ccaatttagc tataccatct
    15061 aaatcttcat aagtctcagt tggtaaacca atcataaaat acaacttaac actattccac
    15121 cccatctcaa aagctctttc tgttgcattt tccaaatctt cttctgtaac acctttattt
    15181 ataacatctc taagtctttg agttcctgct tctggagcaa aagtaagtcc agattttctt
    15241 acttgttgta ttttatcagc aatttccatt gaaaaattat ctaatctaag ggatggaagc
    15301 gaaatcccaa tattattaga tgcatattca tctaccaaat aatcagtcaa ctctgataac
    15361 ttactataat cactcgtact aagtgatgat aatgatattt catcatatcc agtattctta
    15421 actaatttat ctaatatttc tttaagtctt ttaacactct tttctcttat tggcctgtat
    15481 atcatacctg cttgacagaa tctacatcct ctagtacatc ctctaaataa ttctagtact
    15541 attctatcat gaacagtatc tatataaggt actattagtt tttctggata atctacagtc
    15601 tcaacatctt ttattatcct tttagtagga tttttagtaa tcccttctct attaggagtt
    15661 acactttgaa ctgtaccatc ttcattgtac ttgacatcat aaaaacttgg tatgtatact
    15721 ccttctatgc tagatatttt atataaaaaa tcttctctag tcgttttatt tttcttccat
    15781 tctttatact catttactac ttctaaatta acctcttcac cttcacctaa tattactaca
    15841 tctacaaaat ctgctaatgg ttcggaatta taagcacaag gaccaccaac cattataaat
    15901 gggtcttcca aagttctttc ttcttttaat attggtatat tggctaaatc taatatattt
    15961 aagatattag tgtatgaaag ttcatattga agtgtaaaag tgacaaaatc aaagtttgtt
    16021 ataggctctc ttgactctaa tgcaaataat ggtatacttt tttctctcat tatattttcc
    16081 atatcaactg caggagaata cactctctca caaaatacat cttcatcctt atttattaca
    16141 tcatataata tatgactccc taagtgactc ataccaactt catataaatc tggaaagcaa
    16201 tgtgcatatc ttatcaattc actattacta gtatctttat gaattgaatt tatttcattt
    16261 ccaagatatc tagcaggctt ttctactttt tttaagattt ttttcaagtc tactttattc
    16321 atttattaaa attcctccaa acaagctctt tatttttatt cttatttata agtaatatta
    16381 tacacgataa aatattttct atcaaaaata taactaaaat attgaattta aaatacatat
    16441 tattcacaaa atagaatagc taaaaatatt taagaatatt ttctattaaa tttttattga
    16501 aatacaattt ttttacaaag tatgatatat tataaacata aaactattgt tttacatgac
    16561 ttatcatgac atcttttgtt tcattcacct caaaatgggg tatgttactt atataatgat
    16621 aaattttagg agggttaaaa atgagtaaag tttttgaagt aaaaaaagat atctacttta
    16681 ctggagtagt agatgaaggt ttaaaagtat tcgacataat aatggaaact gagtttggga
    16741 caacttacaa ctcttattta atcaaggacg aaaaaacggt tttatttgac actgtaaagg
    16801 caaattttaa agatgagttt ttaagtaatt tatcagaggt tactgatata gctaaaattg
    16861 actatgttgt aattcatcac acagaaccag accatgcagg tagtttaaaa tacttattag
    16921 atataaatcc taatatagaa gtttattgta ctaaagctgc taaactatac ttagatggtc
    16981 aaataaatag accttttaac tgtcatgtaa taaaagatgg tgaaatttta aatatcggta
    17041 aaagaaatct tagatttata actgctccat tcttacattg ggttgataca atgtttactt
    17101 acattgagga ggacaagact cttttaactt gtgatgcatt tggttgtcac tttgcaagtg
    17161 ttgatgcaga ggtagtgaat agtgaagatt atctaaaatc agctaaacac tactatgatt
    17221 gtatagttaa gccatttgct aaacatgttt taagtgctgt agataaggtg gtaggactaa
    17281 acatagaatt tgatactata ttgacttctc atggtccaat gcttactaaa gacccaatgg
    17341 ctgctgttaa aagatatgtt gagtggtcta cagaagctgt taatacaact aatcaaaatc
    17401 aagtttctat attctactta tcagcttata gtaatacttt agagatggct aaaaagataa
    17461 aagaaggact tgataaagaa ggtgcaaagg ctgaattata tgatttagaa gatatgacat
    17521 taacagaaat gcacgataca ttagtagtgt caaaagtaat attactaggc tctccaacta
    17581 taaacaaaac tatggttaaa cctatgtggg atttattctc tgttatagac ccaatggcta
    17641 accaaggcaa gatagcagga gtatttggtt catttggttg gagtggtgaa ggtataacta
    17701 tggctgagac tttacttaaa tctatgtcat tcaaaatgcc tgtagaatct cttaagaaaa
    17761 aattcttccc tagtgaagag acattaaaag agtgtatggc atttggtgca gaatttgcaa
    17821 aattagttaa atagccatta atcaaacata atattattta aaataaatga ttttatattt
    17881 aaaaataaat taaaatggag ttgtctcaaa attaattttt ctaatctaat tttaagttca
    17941 actccttttt tattttccaa aatgactaat ataataataa atgtaccctt tcgcacaggg
    18001 tgcattattt atagtctgtt ctatttgtat actagtatac ttgtattaat tcacgtatct
    18061 agtatatgct actagcccta ttccagaatt agatgttgtt tcaccaattt ctactttaat
    18121 aattatatta tttttttaca cttttttgat aattctgctg cagaaaggtt cttaccagta
    18181 aaatctaaag aaaatgcctt atcatactca aaaggataac taacaggttt gctctctggt
    18241 gctaatgcaa ccataccata tggtgtattc ataaacccta ttacagtaac tgtatgcttt
    18301 gatgggtcta aatttgaatt tttgcttgta aacttaattg tatttattga tgagttgatt
    18361 aaactacacg gaactccctc atacatttca atttttggac gtagattatt tccataaact
    18421 tcatcaagta ttgccccttg ttgatctgct atcatagtgt gattcccagg tccaactggc
    18481 gcagaaggct tagctggcgt aggtttctta acagttggcg ttggattctg tgtctttact
    18541 acttcgccaa cagctggata tgctttcttg tcaaatggta atgggtgagt aagactcttg
    18601 tatatgatgt ctgctcctgc tgatttgttg gcatcaatat ctacatgata caaacgtcca
    18661 tcctcaaaac gaataagatt ccactgatgg tcatttgttg tactatgtac ataataacat
    18721 tcaattccca tagcatcaca taaagctttc aagctcctac acattccaga acaagcacct
    18781 tttttagtaa ccaatgtata agcagtattg gctccctctg ctgcgttcca gttaacatat
    18841 gttgtgttgt ttttaatgtg attatgtata gatacaatcc actatggaga aatataaaag
    18901 aaaagaatat aacaaacaat aaaaaataag atgattcaaa ttttattttg aaacatctta
    18961 tttactctac aaattaaaat tatttggtga attatttaat tattattttt tattaaagaa
    19021 attttaaatc ttaatataaa atacgctatt atacataata atccaaatac gaatgctact
    19081 ggaattaaca tctccgattc aaaataaaag aaatattcta agaaaatatc tggatttact
    19141 atattcccat tcttgtctaa aaaatctttt ccattatatc ctaaatgctg tataaaatta
    19201 cttatgttat tataatctgc ttccctctca tataccatat tagcattcaa tccagaagaa
    19261 cttaaattag tgtttccatc tttcatatct attacatata gtctaagagc tttgacttta
    19321 ctatacttat ctttatcaag tattttttca agttctaact tatattcacc aatctttgtc
    19381 atatcatgtc tttctgccaa ttgctttatt tctccatcaa gttcttttcc taaattatac
    19441 atattatgtt gtaaactatt attgtaatac cacataaatc cagaaattaa aaatgcaatt
    19501 atacttatgt aacctaatac ttttttaaac cccattacaa ttgatttatg cctatccaat
    19561 gataaagata gttcttttat tatattacat aattcatact gcatttcatc accatcatca
    19621 aatctttcaa ttgctttttt acatgcttct tcttcatcta atccttctaa tttaaattca
    19681 tttgcagatt ctataagatg gcatctcatt tcctgtttta attctatagt acttttattg
    19741 tcacattttt tatatagttt atctaaatat tcatctatta gtttcataaa ttaatccacc
    19801 tctcccaaaa attgattcat aaccttttta ataaatctcc attcttgttt ctttatttct
    19861 aaaaaatctt taccttcatt agttaaatta taatattttc ttcttccacc tgaactttgt
    19921 tcattgctat aataagattt aattaaattt ttagattcca ttcttttaag tgcaagatac
    19981 attgttcctt cttttaattc aaactcactt ctttctctta cttgctttgc aatttcatat
    20041 ccataacaat ctttcttttc taatatagaa acaattaata tatcgatata accttttaac
    20101 acttctttat ttaactgcat tatgtaccac cgccttttca cctactactc tataatgtat
    20161 agtacttata tttactataa tattacatag tactctataa tacaaggttt atattctaca
    20221 atagacttaa aaataataaa tagctgtata ttaatattta atctataaat actaatatac
    20281 agctatttat caaattttaa aaataaattt ttgttttatt aatgttgtca taacaacatt
    20341 tacttggttg aaatagtatt acctataagt ttaaatatat ataataatag acttgattct
    20401 atttcatatt attaaaatag agtaatagtt taaattctac tactctatct ataatctatt
    20461 tcctaattat ttaatctcaa atttagctct tcatcattta aatctttcat aagatttcta
    20521 caggtactag atttattcca acagaaaaac tagggcatta aacacttata tatctgtttc
    20581 tttagactgt aatattaata ttttttaaat acgttgactt ttgctttttc taacttctct
    20641 ccatgatagt tctcctctat caagagcttt tataaatatt tctgctgaag ctagatttgt
    20701 agctattgga acatgataaa catcacaaag tcttattaac gcttgtatat ctgtttcatg
    20761 agcctgagat gtcaatgggt ctcttaaaaa aataactaaa tctatttctt gtgttactat
    20821 taaagaacct atttgctggt ctccacctaa aggaccagat gctaatctat ttatatttaa
    20881 ttcagtttca tccattattc tttttcctgt agttccagtt gcatatagtc catacttttt
    20941 taaaattgat tcataaccta tacaaaatcc gaccatagtg tttttcattt ggtcatgtgc
    21001 tactaatgct atattcatat atctaatctc ctcaatttat gtttagatgt atttaaattt
    21061 agaaatttat tttctttctt ctcatacaca catttagtac taatcctaag tttgctaaag
    21121 atgtcaataa cgaacttcct ccataactta caaaaggtaa tgttactcca gttactggta
    21181 ttaacgcaac cgtcatacct atattttgga ttatctggta accaaacatt cccataacac
    21241 caaccactat gagggttcca taaaaatctt ttgcatctct tgcaattgct aaaagcctta
    21301 ataggaatat cataaataag attatcaaca ctgccattcc tattacacca agttcttcac
    21361 ctacaacagc aaatataaaa tcactatctt gtacaggtaa aaaatcttct tgattttgac
    21421 ttccattgta aagtccttta ccagttacgc ctccagaacc aattgcaatc agagattgca
    21481 taacttgata gtttcctttt agagttacat cttctggatt tagaaaagct tctattctta
    21541 ccttttgatg gtctgccatc attaaataca tcagtggcat tgacactaat agtatgatta
    21601 ttcctctttt tatcaacttt gaactaagac ctgctgtgaa cagcattgca aatatcatac
    21661 aacaaaatac aattcctgtt cccaagtctg gttgtgcaat caataatccg ataaatggaa
    21721 gagaataaac tacaacaggc ataacttcct ttaacgtatt taatttatcc tttttggatt
    21781 caagtatctt tgcataactt agtacaaatg ttaatttgac tatttcagat gtctgcaagt
    21841 caagtggtcc taagttaatc cacgacctag ctccaccttt tactgtccca attccaggta
    21901 ataaaactat ggctaacaaa ataagactta ttatataaag cgctttataa tacctgccta
    21961 gaaggttata gtcaaaaaat aatatgacta taatcattcc tataccaagt acaaaagcta
    22021 atccttgttt aatcaattga ttatatgaac ctgttgaatt agcatgtgtt gcactactaa
    22081 gtataacaag cccaaaaata aagatagcta atactgtaac tataagtttc cagtctaact
    22141 gttttatcaa cttaattgtc gacttataat taatattgat ttttttcaaa gaatcaccgt
    22201 ctttctatct ttaaacatat aattaaataa aaggggcata taccaataag gatatccccc
    22261 ttttaagcac taattacata catctatctt ttatattctt tagtggtata ttagcaacta
    22321 atgcagatac aggtttgtca tcatgctcac ctctgcattt agacattttt atttcaagac
    22381 catcatcttc tatttctgca taattagcta ttactttcaa aatgtctgtt tttatcatct
    22441 ctaaaagttg tggtgaacaa tctactctat catgaactaa aactagcttt aacctctctt
    22501 tagcaacaga tttactagtt ttagcctcgt tagaaaaaac tctaaataaa tctaacacag
    22561 atgtatcctc ctttgctact tagccatgct aaacattttt ttaagtctac caaagaatcc
    22621 atcttcaact tcaagatcaa gtagaggaac ttcttcattt agtatccttt ttgcgatatt
    22681 tttgtatgct tgaccagcaa gtgacttaga atcaagtata gctggctctc ctttatttgt
    22741 tgatataatt atgctttcat catcaggaac aagacctaac aaatctattg ctaatatttc
    22801 tattatatct tgtttatcca tcatgtctcc acgcttaacc atatcatttc taattctatt
    22861 tataactaat cttatctctt ttatttcatt tgcttctaat agacctatta ttctatcagc
    22921 atctcttact gctgatattt ctggattagt aactacaata gctctatctg ccccagctac
    22981 tgcattttta aagccttgct caatacctgc tggacaatct attattatat actcgaaaga
    23041 ttcttttagt ttctcacaca ggtcaatcat ctgctcaact gagacagcat ttttatctct
    23101 agtttgtgct gctggtaata aatatagatt atcaaatctt ttgtctttta ttagagcttg
    23161 cttaagccta caagttcctt caactacatc tactatgtca taaactattc gattttcaag
    23221 acccattact acatctaagt ttctaagtcc aatatctgca tccacaacta ctgttttttt
    23281 attttctagg cttagcgcag ttcctaagtt tgctgcggta gtagtttttc caaccccacc
    23341 tttaccagat gttataacta taacttcgct cataatacta ccccctattt ttctttctat
    23401 ttgtctaact ttgacaaata gctttcgatt acaattcttc cattacttac aaaggctatt
    23461 tctgggctaa tttctgattc actttcataa ttttcttcat catctggagc ttctgcaata
    23521 ttatctgcta tttgtaatat tttaggatta agatttccag caacaacata tgcaaattca
    23581 ttgccttctg cacctgcatg tacaaaacct tctattttac ccattacaaa aacattacct
    23641 cctgctacaa ccttagcacc agcatttata tctcccataa caactgtatt tgattttgaa
    23701 aggacttttg aaccaggttt catatcattc aatataacta catcaccttc aaattcaata
    23761 ttttcaccag atcttagggt attgacatat tttgttggaa aattactatt ttcttcaatt
    23821 ttttgatctt caacaaattc cacatcaaat ctagaagtaa ttccttcttt aagttctaga
    23881 atctccatgt cagttaaaca atcactatta attttactaa ttttagcccc attaaaaaag
    23941 ccaacatatg cttccaattt gtttattatt ttttcctgta tttcctcaaa tggagcttct
    24001 ctttttatat ttactattat ccctctttta ttacccttga actctactag ttcttgacta
    24061 caaatttctc ttaaagacat aacaacctcc agagtgatat atcatttata ttcttcaaat
    24121 acgctttaaa cccttttatt ttttctatat aaagctaaag tttatacata gattgagcta
    24181 tctattaatt tgtgactcga aatttatatt ttcgtcagaa gaattgttaa catcattcgt
    24241 cttgttgtca tttttactat tagttggctc taatcccata taagttccaa taacttctct
    24301 agttggtaaa agtgagaata cacttgaatc accttgagga attacacata caacagctat
    24361 ttctgggtca tctgctggag caaaagcaac agtccacgta aaagaaccat aatcttgttt
    24421 aaatctatct atttggtcat ctgttatttt tggatttaat tcttttatag cttttcttag
    24481 atatgaagaa ttcaccttat cagtgtcctc taatctaact tttactccat cttctaattc
    24541 ttcctctaat ttctttcttt cttcgtcttt caaatcttta ttttctaatt tcttttttat
    24601 ttccttttcc ttagctaatg aaagctcttt ttctttttct gctttcatct tatcagctag
    24661 tttaatcgca tcatttaggt tgacattata agatgacata tgacttttta aatattcata
    24721 ctcgttatct gtaggtattt tcccactttt ctcagctgta ccagtttttg ctgctacatc
    24781 tattggaaaa tcagcaaaag cactcttcgc tgtaccttgt cttgcaacca atttcatacc
    24841 ttttgttaat tcttttaagt tatctggatt tttaaaaggt attttttcga ctttcttctg
    24901 gtcattaatt ttaactgatg aataatcact tgaaacagct ctatcaacta cagataattc
    24961 tactaaatta cctccatttg ctatagcagc tacatatctt gatatttgag ctggtgtata
    25021 agcattttca ccttgaccta tagctaagtt aaatgtatct gcagtactcc attttgcaaa
    25081 gttaaaataa ctaaatactg ctaaatctgc tacttcttca attctatctt ctttaacatc
    25141 caattttttt agcctattca ttgtttcaac tctaccagga gttttctttt ctgctgccca
    25201 gcttacaatc tcattgattc ttttctcata ttctttagga tttttttctc tagttatatc
    25261 aacaaaatca tttgccattt ctctttctaa atttgacttt agaagagctt gtgttgattt
    25321 caatttatct tctgtgctag gaactttacc tgctctttct tctacttcat cttgaagccc
    25381 tgtattttgg tcaagcccaa ataattttgc atactcaagt attttatttg cattcatatc
    25441 tataccaatg ctttttccat ctggccatgt tttacctgta ccaattgttg ccatatatat
    25501 attacaagac tcttgtattg ctttatataa gtttgtcata ccatggtttc ctctaccatg
    25561 gttccataca tagtcaccaa aagatttttt acctaacatt ataaccccag ggtcatttat
    25621 agtaaagttt ggatccagac cattttctaa cgctgccatt gaagttacca tcttaaatga
    25681 agaacctggc tgaaattccc cttgtgtaac caagtttaaa agtggacttc ctgctagtaa
    25741 gtcatttgga ttttttggtt gaagtgcttt ataatcctct gtagaaatac cttttgcaaa
    25801 tttatttggg tcataattag gataacttga tgaagctaat acttctccat ttttaacatc
    25861 aatagctata agagctgctg actgtgcttt tcctgcatat gcactaattg gtttgtcccc
    25921 aaatttactt ttaaaagtac ctcctttaga agcaacttcg attatctgtt ttaatgcatt
    25981 gtctgaaact tcttgtaaat ctttatctag tgtaagatac acagtatctc ctgatttagg
    26041 ttttttagat tcaatttctt tactgattct acccaatgca tcaactttaa ccattttata
    26101 accatcagtt cctcttaatc tactttcatt actcttttct actcctgcaa gacctaccat
    26161 atctccagtt tcataacctt tttgaaggta tgattctatt tgagtggatg gcatttttcc
    26221 tacatatcct aacatatgag aagctaatga cccattagga taatatctta ctggttctac
    26281 tgctattgaa acaccaacaa ggtccatagc actttcttca atttgagcaa tcgtagtttc
    26341 tcctacatcc ttagcaatag ttactggatt atattgagaa tatccttttg attttatcaa
    26401 atcccttacg accattattt ttcttgcatc ttcatcactc aaacttttat caatttctaa
    26461 tgcatcacta tttcttactt tttcaaacgc ttctttggca ctcagtttag tttcttttat
    26521 cttataacta gccaaccaat ctctcttatc tctttctgct gtgaacatcc atttactaac
    26581 taaaataggt ggataatatc cattttcatt tagttttgcc tgtaatcttg tcgcatccaa
    26641 tcttttatct tcttgagata atattccagc tgatataagt ttgtccacca agtaataaaa
    26701 actttcctta gcattataat catttggaat accattttct gatttatatt ccctaatatc
    26761 tctgtcatat gtataatagt actttccatt ttctacatat ataggaaact catctacata
    26821 ttcttcacca tttctctcca gcaaatttat aagttttaat gatatttcat ttgctcttga
    26881 atgcttattt gcatctttct tattaatatc gttacctgaa acttgaacag taaataagtt
    26941 tttatttcct gccaacagtc ttccatatct atcttttatt tctcctcttg gagcttctat
    27001 tgccaacttt ttatatgttt tattctcagc taactcattg taatattcat atttaaatgt
    27061 agtcatatag attattttta ctaaaatagc taaaaatatc acgcccatga ctatcttaat
    27121 tatttttagt ctatcaactt gtttattttc ttccatgaaa tcaccaacta atcttcttta
    27181 agttttaaaa ctgctctttt agataagcga tatattacta aaccaattaa actattcact
    27241 attggtgcta ctagtagacc ttttacaaaa agtgttgata tgttgtagct ttgataaatt
    27301 atactagatg tacctatatt gacaattgaa tcaattaaac tgcctataaa aattaaaacg
    27361 aaagtaataa tataactacc tttatatagc tgatctctca tgtatccaat tgcatatgca
    27421 ataataaaca aaattaatgc atttacacca aaaattccac caacggttat gtctttaata
    27481 agccctatta ttgctgctat aatcccacct tctaattcat ctaaataaag agagattatg
    27541 gttacatata taagaactaa attaaagctt atgccaaaaa tatctatata atttgttata
    27601 ctattttcta tagtcataag cacaatcccc aacagacaaa gtaaaacttt tttcattgtt
    27661 tttatcccct ttatcctata tttcttggtt ctatgactac aacgtcattt atattcttaa
    27721 aatcaacata aggttttaca actacatatt ttaatgactt atctttatca tcaaccactt
    27781 tttcaacttc gccaattggt ataccatctg ggaagaatcc aagacctgat gttgttatta
    27841 tatctccttg aattacatta tatgatgagt caaacatata tccttgcaaa tatcctttac
    27901 tattataact atctttatta tctaaagtag tattttgagt aattgtacct ttagcattgg
    27961 catcctttgc tagtttaaag cttacagatg ccttagaatc caatagtgaa attgccttac
    28021 tataattgtt ggaaacttca taaactatgc caactaatcc actaccattc ataactatac
    28081 tttctttttt tacacctgaa ttttttcctg cacctatgac aaaactctca taccagtttc
    28141 catcattttt accaacaaca cttgctgaaa tacttgtcgc cttatactct tcttgtacaa
    28201 agttcagtgt cttttttaac tcttctagcg attctgtttt atctagtttg gcatttaaag
    28261 ctatcatatt ttttttaagt ttttcatttt cttcttctaa tttttttacc tttttagaat
    28321 tatctttaaa atttgctaca tctttaaaac catttttgat aaaagaaaat ccatcattta
    28381 ttcccttacc tacacttgta atagcatctg aagctactcc taaatttact ggactaccac
    28441 tagaaaattt accaattgat atacccacaa ttccaattaa agtgatagca actacgcccg
    28501 ttgctatcac tttaacatta atcctttttt tctcattttt gttcttgtca aatctcaagg
    28561 ccatcaccaa cttctaattc ttagtattca tcattaatac tttttcaaag atttcttggt
    28621 cttcaacaga tttacctgtt ccaagtgcaa cacaatcaag aggtgtttct gctatttgta
    28681 cagacatacc agtttcctgt gtaataagct tatcaagacc tctaagcaaa gcgcctcctc
    28741 ctgttagcat tattccgttt tccattatat ctgaggctaa ttctggtgga gttctttcta
    28801 gtgtagattt tattgcatct actattgaac ttacaggttc ttttaatgct tctctaactt
    28861 ctgtagagca tatttctatt gttttaggta atcctgagat taaatctcta cctcttattt
    28921 gcatatttat ttcttcatca tctttaaatg tagaacctat gttaattttt acattttcag
    28981 cagttctttc acctatcata agattatatt ctttttttac ataagccact atagactcgt
    29041 caaattcgtc tccacctatt cttatagatt tagcagtaac gattcctcct aaagatatta
    29101 cagctatttc tgaagtacct ccaccaatat caactaccat attaccttct ggttcttcaa
    29161 cttttaatcc agcccctata gccgctgcca ttggctcttc tataaggaat gcatctttag
    29221 ctcctgcttg tcttgcagcc tcttctatag ctctcttttc tacttctgta actccaaaag
    29281 gaacacatat agctattctt ggacttacaa cacctttttt atcagccgct ttttgtataa
    29341 aatagctaat cattgattga gttatgtcaa aatcagctat tactccatct ttcataggtc
    29401 ttatagctac tatatttcct ggtgttctac ctatcatttt tttggcttct tcacctactg
    29461 ctaatacctc tttactatca tctcttattg cgacaactga tggttctcta acaacaatac
    29521 cttgtccttt tatgtaaact aatgtatttg cagtccctaa atctattccc atatcctttg
    29581 tcatcttatt aaaactaaaa aaaccttttt tttctttctt tttttcctta gccatgattt
    29641 tactccttcc tactattaag aatcatgttt aaaacattct tctattattt tatcacatat
    29701 aaagtcaaat tatcatattt tctttaaagc taaagtataa tccatctcca ataattatat
    29761 ggtctattat ttctattcca attatttctc cacatttaat aagtcttgat gtgatatttt
    29821 tatcttctct tgatggttct acacttcctg aagggtgatt atgcatcact attatcttac
    29881 ttgcacttct tcttatagct tctttaaaaa cctctcttgg atggactaat gacgagctta
    29941 aagtccctat agatacatct acatcagata ttatctcatt ttttgtgttt aaaagaaccg
    30001 ctttaaaaac ctcttttttt aaatacctta ggctatccat ataatacctc tgtatatccc
    30061 aaggattcct tattttatac tttataggtt taaaactagc tactctagag cctagttcta
    30121 aagctgcttt aatttgggtt gattttgata acccaattcc atctatatta cacagctcct
    30181 ctattgtcat atcttctaaa tgtctaatac cctgaatatc tctatttatt atatagttgg
    30241 ctaattcaat agcattttta ttcttgttgc cagttcttaa aagtatagcc aacaactcag
    30301 catttgataa gctttttacg cctttggcaa gcatcttttc tctgggtctt tcctctggtg
    30361 ccatttcttt taccttaatg gttgaattaa atgacttttt cagataaatc acactccata
    30421 aaaaagattt atactaaaat atttttttaa taggtcgcct aaccttgaaa ttggaagacc
    30481 aactatatta aaataatctc ctctaatttc ttcaactaac aaagctccat aaccttgtat
    30541 accataggct cctgctttgt caaatgactc attagttttc agataatcct caatatcctg
    30601 ctctgataac gttttaaatt tcacattgct gattacatag tcaattacct ttttatcttc
    30661 gcacaagttt attaagctaa tacctgttat gacttgatgc tctcttccag atagtctttt
    30721 taacatatct cttgcacaag actcatcttt tggttttcct aaaatagcat tgtctaaaac
    30781 aacaactgta tcagctccaa taacaacatc ttcattatgt tcagatgcta cagacatact
    30841 tttttcaaaa gctaatctca taactaaatg ttttggtgtt tcaccttcca atataatctc
    30901 atcaatttca tttttaatta tatcaaatct tacattcgta ttctctagaa tttctttcct
    30961 tcttggagag gctgatgcca agattatatt catttattca cctcatttgt acgtaatttg
    31021 tacctattta gtctatcact atattgtaaa aaattcaaca gaaatgtcga accttttttg
    31081 ttacactatt gttatattta ttaccattag aataaatata cataaaatag aggtatggaa
    31141 attattccat acctctgttt tatgtactca ttagagatat ggaaattatt ccatatcctt
    31201 attttgtcca tattacaaaa ttttaatagc tttctttgga catttttcca tacataaatg
    31261 gcatcctaca catttatctg catctacctt atgtttttct tttaattctc cctctattgc
    31321 atcaaactta cattgttttt tgcacactgt acatccaaca caaagttctt ggtcaatagt
    31381 aactttcttt ttcttagtta tgtcacctga tattactttt gtagggcatt ttgctacaca
    31441 ttgcatacaa cctacacact tattagggtc tatcttagct attttgtctt caaatattat
    31501 tgcatcaaat ttacaagctt ttacacacat tccacagcca atacatccag cagtacattt
    31561 ttctttaaca gctttaccaa actctttgct attacactct acaactactt cttgactttc
    31621 tggttttgtg attattatac ccttaggaca aacttcttta cattttccac aatttacaca
    31681 cttttcttca tctattactg ctattccatc aactatagaa atagcatcaa atttacaagc
    31741 atctttacat gttccaagac ctaaacatcc aaatttacac atcttagcac ctgagtttaa
    31801 aacatttgct gctctacaat ctgatattcc ttcatactca tatttatctt ttgcacttga
    31861 acaagttcct ttacaaataa cttttgctac ttgtttctca cctgcttcag cacttattcc
    31921 cattatttct ccaacttttg caccaacatc tgctccacct acagggcaac cattaactgg
    31981 tgcattgcct tcaactatcg catttgcaag acctccacaa cctggaaatc cacaacctcc
    32041 acagttggct cctggaagta ctcctagtat agcttccact ctctcgtcta cttctacagc
    32101 aaacttttta gatgcaaaat ctagtactat cccgaagatt aagcccataa ttcctaaaac
    32161 taatacagct gtaagtatca ccattttcac ccctcttttt tatagcttta taagtcctgt
    32221 aaatcctaag aaagctattg acattaaacc tgctgatata agcgttatag ggaaaccttt
    32281 aaatgcttct ggtatatcag ctaattctag tctttctctt atacctgcga atattactat
    32341 tgctaaagta aatccagctc cggctccaaa tccgtttatt attgtttcta ctaaattata
    32401 acttttttga acattaacta gagctatacc aagaactgca cagtttgtag ttataagagg
    32461 taaataaact cctaatgctt gatataaaga tggactcatc ttttgaatta ccatttcaac
    32521 gaactgaact atagaagcta taaccaatat aaaagctatt gtttgtaaga atccagttcc
    32581 agtttttact aataaaatct gtataaaata agttataatt gaagctagag ttaaaacgaa
    32641 cgtaactgca actcccatcc ctactgctgt atcaaccttc ttagaaactc ccaagaaagg
    32701 acatataccc aagaattgag atgttataac gttattaaca agaacgatac ttaaaaataa
    32761 aagtattaaa ttcatttatt acacctccac tatgcttttt tacttcttaa tttattaaat
    32821 cctgcaaata agaatcctaa tgttaagaat gcacctggtg gtaatataaa tattaatact
    32881 ggttggaaag atgctccaaa taatgtcatt ccaaataaac taccatttcc aagtaattct
    32941 cttactgcac ctattattgt aagtgcaaca gtaaatccaa gaccttgacc tacaccatca
    33001 actattgaag gcatagctcc tgttttgaaa gcaaaacttt ctgcacgagc taatattaaa
    33061 cagttaacaa ctattaatgg tatatatata ccaagagcct tatcaagagc aggaacatat
    33121 gcttttaaaa gcatacctac tattgtaaca aatgtagcta taactactat aaatataggt
    33181 attcttatct tatctggtgt aatttttctt attaaagata taactaaatt tgcacatatt
    33241 aaaactgctg ctgttgaaag acccatacca attccattta ttgctgaagt tgtaacggct
    33301 agtgttggac acatacctat aacttgaaca aatgttgggt tctcatctat tagcccattt
    33361 ttaaatactt tagctaaatt cataattaac cctccttatt tattaagagc actattgaaa
    33421 acatctatag cagcatttac tcctgtagta actgctgtag atgtaatagt tgcacctgat
    33481 attgctaata tttgattatc tgctgttgta cttccttttg ctacttcaag aggctcagca
    33541 gtttttcctt tgtattgacc cttaaatgca tcatccttag cttttgctcc aagacctgct
    33601 gtttctgaca tattaccaat gtcaacacct gtaacttgac cttcagatga tatacctacc
    33661 ataacttcaa tttctccacc atatccactt ggtttagctt ttaaagtata tccaaccact
    33721 tcagaaccat ctaaaccttc atacacttca gctataagac catctccaag gcctttgtaa
    33781 gtatcttcct tcatttcttt aaattctttt gcttctggaa ggactgcttt tctaagttca
    33841 ttatttgctt gtatatttct ttgttctatt actggagctg taacctgatt agtagctcca
    33901 agagctaatg cagctattgc acttatagca agtaaagtac cacctaatct caccatactg
    33961 ttcattattt agccacctcc ccaaatactc tatttctaac aaacttatct ataagtggag
    34021 caagacagtt cactaatagt attgagtaag atacaccttc tggatatcca ccatacattc
    34081 ttataacagt tgttataaga ccagctaata cagcgtatat tatttgacct tttttagtca
    34141 taggcgaagt tgtataatcc gtaagcatga agaatccacc taacataagt cctccagcaa
    34201 acagttgata tattgcgaaa gttatattaa atccacctaa aaggaatgtt aatataaata
    34261 cagttgctat gtaaaatact ggtataacat aatttattat gcctttatac attaggtata
    34321 ctccacctaa taataataat attgctgatg tctcacctat acatccacca atattcccta
    34381 tcatcatgtc agcaagacta attccattgc tagaaaggtc tgccagtcct gctgctccat
    34441 ttttcaaaaa gcttaatgga gttgctgtag taactgcatc taaattttta cctatccagt
    34501 taacacctgg ttgagtccaa gcagtcattg ctactgggaa tgatgctaat aaaaatgctc
    34561 ttgcagcaag tgctggattg acaaagttac atccaattcc accaaatacc attttaacta
    34621 ctaatattgc aaatattcct cctactagac acatccacca aggaagtgat gctggtacgt
    34681 taaatgctaa taataatcct gttacaacag ctgaaaaatc tcctatagta cttttttgct
    34741 tcgtaaattt ttgatataaa aattcagtac ctactgtacc aagtatacaa aaaaacatag
    34801 cactcaaagc attaagtcta aagaagtata ctcctgctac tgctgctgga aggagtgcta
    34861 taataacttg tttcattata tatgaagtat cttcattact tctcacatga ggagaagatg
    34921 atactatcaa cttattttcc atcgtttttc ccccttaact ttctttattt ctgagcagct
    34981 tgctttcttc tctttgctcc tatttctctc tttgctgatc ttatagattg aagtaatggt
    35041 cttcttgctg gacaaataaa tgagcaagac ccacattcta tacaatccat tgctctatga
    35101 tattcagctg tgtcatagtc atcttttaaa gaatatgcac ttatgaaaag tggttgtaag
    35161 aatgatggac atacatctgt acatcttcca catcttaaac agttttgaat atctggagta
    35221 cgtgattctt cctcatccaa acataaaatt cctgaagaac ctttgttagt cgctatttca
    35281 gtagtatatt gagctattcc catcataggt ccacccatta taaccttacc aatcttctta
    35341 tcttctttaa atccaccaca ttgttctatt atttctgaaa ctaatgttcc aacttttgtt
    35401 attaggttct ttggctcttt gatgcaacta cctgttattg tagtgattct ttcaaccaaa
    35461 ggcatacctg ttttaataga cttagctatc tgtgctgctg tggcaacgtt gtcaacaact
    35521 gcacctgctg ctattggtaa tccaccagat ggaacttctt tgcctgtaca cgcataaata
    35581 agttgtttct ctgctccttg tggatatttc acttgaaggc caacaacttc gatttcgcta
    35641 tagtctttag ctacattttg aattgcttct atagcatctg gtttattcgt ttcaattcct
    35701 ataaatcctt ttttgacatc taataccttc attaatgctc ttaaaccaaa cacaacatct
    35761 tctggatttt caaccattaa tctgtggtct gcagttaagt atggttcaca ttctgcacca
    35821 tttaatataa ctacttctgc cttgctatct ggtggaggag atacttttac atggttaggg
    35881 aatgtagctc cacccatacc tactattcct gcctctttta tgatacctac tatatcttct
    35941 ttgctcaaat cctctaactg accttttggt tgaacactct catgtaattc ttccttaaag
    36001 tcattttcga taactacaca ttgagctgaa cctcctggta cttcatgttg ttcaatagca
    36061 ataactttac ctgatactga agaatgaacg ttacatgaaa caaaaccttg ttgttcacct
    36121 attttttgcc caaactttac ttcatcacca acttcaacaa taggtttagc tggtgcccct
    36181 atatgttgct gaagaggaat gtaaactatc tttggagctt gagctttttc aagtgccttt
    36241 gtgttagagt actcttttct atatggagga tgtatccctc ccttaaaagt taagagtttc
    36301 attgttttac ccccttatct ataaaaaaac attttttctg ccttctataa gtatactata
    36361 tatattaaaa aatcttaaca attgttaaaa aaatatccgt atttttataa tttattttat
    36421 aaatcaacac ctctatatta agtcaaattg aggaaaagga ttgcttaatt aaacaatcct
    36481 tttcatatag ctttctaaag tccaaatttt ctgtatttta ttttatagaa tttataaaca
    36541 tagagtactc ctgcatggat gataaaagca gacttttact tacactatat ttttcttcat
    36601 ttgctgcttt tgaagctgta tcaatttttt cattcacatt ctttacatac tctattaaat
    36661 tatctttaaa taaacgcatt tcagaataat caatcttttc agcttctttt gatatatttt
    36721 gtgatattgc ctttctattt gtcaaaatag tattatattt ttccatatct acattatttt
    36781 cagcattcga ccaaaaactt gattcttctt taaagtttgt tattaattta tttaattctt
    36841 tagatatact ctctatataa gcgtagttac ttgtatactc taaagacaac ataggtgcat
    36901 ctaagtgtga taaaaatgca tctggaaaca cctttcttac actgcttatc tgctttctaa
    36961 catcattttc atcaaaagag gaataggtct gtattttttt tactccatcc ttctccataa
    37021 ctgaaaaagg tatgtcatta ttaactaatg atgtctgtat ttttttcaaa tcatcattat
    37081 tatctattgc agcaacttga atagtataca tatcccagcc ttctatagta gctaatttga
    37141 cttcttctgg ctctttagtg ttatttaccg ctttttctgg attagtttct tgtttatcat
    37201 ctgaattggt ttgtgattta gattttttcc ctttttttat tgaatttaat tcatcagata
    37261 tatctttata agttatatct gatgaattat ttagaaatgg tattttagcc gatacatact
    37321 ccaatatctt gctatctttt atttttgtat aagaataata gccaattaga cacaagcaag
    37381 caatcgttat aacatattta cgtaaatcaa tcttatgtct attattatta aatcctctgt
    37441 atatcgtttt tcttctattc attagtttgt ccctcccaaa attatattat taataaagta
    37501 tatttttaag agtcacaatt tatgaatata aataattatt tcttctaatt ttttaataag
    37561 aaattgtact tactaaacaa atcttcttgc accaagtaat actttagacc aatatgaact
    37621 agttaaatta tccactttaa ccttattagg attagtctga gtaccactac aatggataaa
    37681 tttactattt cctacgtata tacctacatg attagttgtt ccatcaccat cagtgtcaaa
    37741 ataaactaaa tctcctggtg cataatttcc catagaaatt gcacttccaa aattagcctg
    37801 ttctctagaa actctaggaa tagaaacgcc tacagaatgt ttaaaaacat actgtgtaaa
    37861 tccagaacag tcaaatttat caggtcctga agctccccat tgatatggta ttccaataag
    37921 agtatatgca aaatctacga ttgttgaacc ttttgctgta gatttatctg aatttccagt
    37981 aaatgatggc tttgagttac tattagattg agaattttgg ttagatgatg aatttggttt
    38041 ttcttttgta tcctcattag tctcagaaat ataactggca ctcacccatc caattgttcc
    38101 actcgataac tttaccttat accaaccatt acttttagct tttaattcaa caacatctcc
    38161 accatttaat tttcctatta tagaatagct tgtcccagga ccacttctta catttaaact
    38221 cacagcagat gttaccttac cattcttgcc ttcaattgat tcttcgctag atggtttttt
    38281 atcagaatta ttagtactat ttgaattact agaattgttc gttccaacat cttctgatgt
    38341 tttagaaata tattggctac tagcccatcc agtaactcca tttgatagtt ttattttata
    38401 ccatccattg ctttgttcta gtaactttac tacatctcca ttatttgctt taccaactaa
    38461 tgaatagtta gtacctgcac cacttcttac attcagtcta gaactcacat ttactttacc
    38521 atttccacta atagttgtcc cacttgaagt tggattattt tgacttgatg taccttcaga
    38581 cgaaccactt gttgatatgt attttgcact tccccatcca acaacagaac tcgataccct
    38641 tactttatac catccattag acttttctaa aatctctaca gtttttccct tgtataattt
    38701 tgctacctta tcgcaatcag aacttggtcc acttcttata ttcaaggcac ttgctgttac
    38761 tgttcccttt tcaagagcac ttgcatttat ggagctaaca gatactgcta ctgccccaat
    38821 tcctaaagct gctatagctt ttttcactac tatcactccc cccaacaaat ttttatagtc
    38881 ataaattcat aacattatta tccattttta gtccctacaa tttaatgata acttattttc
    38941 ttattaattg taaagaaatt ataaacatat ccttaaaatt agtgattttt aggaagtatt
    39001 tcaatccaat aattatctgg gtccgctata aaataaattc ccatactttc attttcaaaa
    39061 catatgcaat ctaaatcttt atgaagtttt tttgcctcat caaactcctc aacttctaaa
    39121 gccagatgaa attcattgtc tcctaaatta tatggtctat cccaatctct aagccaagtt
    39181 aattctaatg tatgactagt aatgccatct cctaaataaa ctaatataaa acttccatct
    39241 tctgcttcct tgcgctttac ctctttaagc cctaaagctt ttttataaaa atctaaactt
    39301 ttttctaaat tagtaacatt gaaattgtta tgacaaaaat taaacttcat tttgaaatca
    39361 ctctccaatt tttatttttt ttatattata tcattattat aaatttattt aaatcatatt
    39421 aagttaaaaa tttgaatgat gatgaccaat taccaaatca tggtaaatta tttaaaagaa
    39481 tatgtcaaaa aaagaataag ctctttcaaa tgcataaaaa atttataccc aagttatact
    39541 taatcatgca aattggcaga acttattctt taaaactcta acttttaagt ttttctttaa
    39601 taatataatt tattactttt tttctaatat cttcagcatt ataatcatct tctacttcta
    39661 tatccaaaag ttttgctatt gacctcatat cttctctagt ctttgaccta tagcttgata
    39721 ttaattcatc ttttattttc tctggttcta aaagatattc ttcatctcct cttttgaaaa
    39781 caacgtattt ttgagcatat gaacttctat tgtagtatat atatcttgat atttttctta
    39841 aaatttgatt atatgaaagt cgcttgttaa tatcaagttt taagtactct gaaaaagcaa
    39901 atagctcttt ttttgattta aataatgctt ctttctttat agttcttgat attgaagtta
    39961 cactttcttg atatgcacac ttttttaaca tttttaaata tactctatta agaccaatac
    40021 tttgattgaa tttttttata ttattattaa taatcttatc caagttacct tcaaagtatt
    40081 tttttgctcc tttttgtgat acataactct caagttcttt gtgggccata tcaagtatca
    40141 ttttttttaa atcctcttta tatcccctag attccatgat actccctccc ttagctattt
    40201 atagtatatt caaaaagctc aagaaatgtg agtcattaca aaattttttt actttttatg
    40261 tatatattaa tttaaaaggg gagataatag taatgtaaaa tcaaacatac tcaatctccc
    40321 ccatttttag acctctatta ctcccccaat agaggtcttt ttttagttta tcaatttcta
    40381 ttcaattccc tttagttcaa atcctaattc tgctatcact tcttttaatt tttcaataga
    40441 tacatcatct ttcatatcta cagatgcaca cttattttct aaatctacat ctaaaacttc
    40501 aataccatct atatcttctg ttaatgcagt tttaagatgg tttacacaat gaccacaact
    40561 cataccttct actaatagtt tcttcttcat ttaattacct ccttataccc tataagggta
    40621 ttctatcata tagtttatac tttttaaata gaaattaatc atttaatttg attcattcga
    40681 ttttatatct aaattagata ttccctcata tacatcaaaa tcatttggag taattttacc
    40741 ttttaatcta ctattactta caatatagtt gtttctcaca gctaaactta taaatgttgc
    40801 attatctttt atatatgtct gtacatcttt gtatgctttt tttgtttcaa tttcagacgt
    40861 agaacttgta agtgcagaca tatatccatt taacttatca tcagtatatc cactactcgc
    40921 tataatacta cttgcatctg gtataattga tagtttccat ccaactaatg ctaaatcata
    40981 atttttagaa ttaagagcat tttccatatc cttatctgag agctgattta ctgtactatt
    41041 tattcctata gcttttaagt tttcatttat tatgtttgca gtttttattc tctcactatt
    41101 atctctgttt actattatct ttaagttcat ttcagaaaga cttttcttta cttcctcttt
    41161 ttctctattt tttttctctt cagcttcttt tttgattcta tcttcttctg cttttttaac
    41221 ttttgcttct tcttctggcg taagcttctt tgagttcttt ttagctgatt ttttctgatt
    41281 ttttacttta ttgtcatttt gatttactga gtttacattt gcatattcta ccttagcaag
    41341 acactctatc gccttatctt tattaaactc taaatccttc atttcactat tatagtattt
    41401 tgattttgaa ttaagtggaa aattaactgg agtagcatca tccatataac cttcactaat
    41461 aattctatct ttatctatag agtgtgcaat tgcttttcta aaattaacat ctttgaaaaa
    41521 aggattatta taattaaata atacacactc ataatctctt ccttgatatt ttgttatatt
    41581 aaactctttt tcttgaaact tacttaaatc atttaatgta acattggtta tatcactatc
    41641 aagagccata accatagatg tccttgcatc ttcattaggc accataccta cttttatgtt
    41701 cttcatggtt tttggaactt cttcatagta atctttgttt acagataaaa ccattccttc
    41761 tctttctgta tatgattcta ttttatattt cccatttcct atcatattat tattcttgtc
    41821 atttacatct tttgaatcta aatgattttg agatacaatt ggaaaaatta aactatcaat
    41881 tgaaaaagca tatctagctt taaattttat tgtaaaatct ttatctgatt taatattcac
    41941 agaagctata tttttagtaa attcattata tggactatct gcactttttt gaattaaacc
    42001 aatagtatag tttacatcat ttgaagtaac attttttcca tcatgccact ttgcatcttt
    42061 tagctttata tctatactca ttccatcctg agctatatta tactccttaa ctaattgagg
    42121 aattacattg tagttctcat ctatagtaaa taaactatca taaactaaat tcattatata
    42181 accaacagac ttatcagtat tagttatagg atttatagtt tttggagtta ccattgttaa
    42241 gtttatatac ttagcatcta tagaactttg actttgtaaa cttgaatcac tactactttg
    42301 ttttgtttta gtattactac aaccagctac cataagagtt atagctaaaa cgactgttaa
    42361 gacttttatt ctcttcatta gttttttcct ttcttgccat atatcttatt ataatgttcg
    42421 taattatttt ttactttttc tacatatttt gaagtttgct taaaaggtat atcatgtaga
    42481 ttttcgccat ctttactata ttcattattt tctaaccatt ttttcacatt acctgaacca
    42541 ccattatatg cagctatcac taaatctaat ttaccaaatt ctttgtataa cttacttaaa
    42601 taccaacagc caagtcttat attagtctct gggtcgaaaa tatccacatt ttttaaattt
    42661 agttcctctg ctccccaatc tctagttatg tctaatattt gcattaatcc ttttgcttcc
    42721 ttttttgaaa cagcagaact attaaactta ctttcggctt taataacact gtaaactata
    42781 ttttcatcta aattaaattc tttcgaatac ttttctacat actctgaata ttttttagga
    42841 tataaaaatt tatgtattac tttgctttcc attaatagtg ccccaaataa gattataaaa
    42901 atagatagaa tcaatacttt cttactattc acgccaattc tccttcatgt actcaataaa
    42961 tttatgtgct ttactttcta attcagttat tgttcccgaa ttatctatta tataatcacc
    43021 atatttggat ttttcttcct gactcatctg tgaatttatc ctactaagtg cttcctgctc
    43081 actgcaatta tctctctttt gtattctact aatttgtact tcttgtttac aagtaactac
    43141 taatagcttg tcaaccatat ctaaaaaacc actttctacc aatattgccg catctaacac
    43201 tactatattt tcgccttttt ttctcaattt ttctatttca cttattattt tttctcttat
    43261 tcgtggatga gtcaagttat ttaattcctt aagtttttct tcatcactaa aaactatttt
    43321 cccaagagct tttctattta gagttccatc atcatttttt atactttgcc caaaatggac
    43381 aaatactttt tccaataaca acttatcctc aaatatcttc ctggatataa tatcagcatc
    43441 cactatagga atgttaagat ttctaaatat atttgatagg ctgctttttc cacagcctat
    43501 tcctcctgta agtcctaata tcaacataaa atcacctact ttgtctcata ccaagaacta
    43561 cctacattta aatctacatc taagtgaaca tccatattta cagcattttc catttcttct
    43621 tttactattt tcttaactat atctatttca tcatcaattg cttcaactat caactcgtcg
    43681 tgaacttgta gtataagttt tgatttcaaa ttgttctctt ccaatttttt atgaacattt
    43741 atcatagcaa tttttattat atctgctgca cttccttgta ttggtgcatt cattgcaaat
    43801 ctctttcctc tatttctttc cataaaattg cttgacttta tttctggtat atatcttctt
    43861 ctgtttaaaa ttgttacagc atatccatcc tcactagcct tttctactgt tgtgtccata
    43921 aactctttta ttttatggta tttagcaaaa taactttcga tatattcttt cgcctttgga
    43981 acaggtatat ttaaatcatc agctagacca aagtcacttt taccatatat aataccaaag
    44041 tttacagctt tagctgcacc tctcaattca ctagtaacat cttccatagg aacattaaaa
    44101 acttgtgaag ctgttttggt atgaatatct tcatgatgtt taaatgcttc tatcatattt
    44161 tcatcctgac tcatatgtgc aagaactctt agctctactt gtgagtaatc ggcatcaacc
    44221 aacttacagt tatcatctgc aataaacact tttcttattt ttctacccat ttccattttt
    44281 ataggtatat tttgaagatt tggttctgta gatgaaattc ttcctgtagt cgttatagtt
    44341 tgattaaatg atgaatgtat tctattactt tctggattta ttatatttaa aagtccttca
    44401 acgtaagtag aatttaactt tactatttgt ctatactcag ttatcttatc tattattgga
    44461 tgcttatctc ttaatttttc taaaacatct gcattagttg aatatccagt tttagttttt
    44521 ttaattattg gaagctctaa tttttcaaat aatataacac ccaattgttt tggtgaattt
    44581 atattgaatg gctctcctga taattcataa atttcctttt ctaatgtagc tattatttct
    44641 ttaaattcaa cactcaattc atccaatata cttttgtcta cttttatacc ttcatactcc
    44701 atatgaccta agacttctac taaaggcatc tctacatgat aaaaaagacc atccatactc
    44761 atatccatta gacttctttc catcatagga actgtttctt taactgtgca tattatttga
    44821 cccatatact ctgctaaatc ctcaaattct aaatcctcat agtttttaga tttaacaccc
    44881 tttcctaata attcttcttt tgatttaacc ttttttgata aatacttcat agctattgca
    44941 ctacaatcat atgaggtaga tgaagttgaa tctattaagt actcagctat tgttatatca
    45001 aaatacatat tttctaagtt tatattatat ggttttaaag atatataatc atcttttaaa
    45061 ttatatccat actttttaat ttcattattt gaaaatatac tatctaactt tgacacttca
    45121 tttgaatcta cataatatat tttttctcca tctacactta taaaaatata tattatattt
    45181 ttttcaagta tattaccttg tcctctaaca gtttttaaaa taacttgtcc ttttttgttt
    45241 acttcatcta taaatttttc tatatctttt aattttaata tttctatttt ttcttctacg
    45301 tgttctgtgt catctgaatc gaccaaatca actaatcttg ataagagact tgtaaatcca
    45361 aagtatctaa atttatctaa aagttcatct ttatcataat cgccaaaaat catactatct
    45421 aaatccactt ctattggcac atctcttata attgttgcaa gtcttttact ttgaatagct
    45481 atttctttgt tttcctctat tttctttttt acactacctt taagcttatc tgtattctct
    45541 attagattct ctatactaga aaactctttt ataagcttta tacctgtttt ttcacctatt
    45601 ccaggtactc caggtatatt atcagacttg tctcccataa gaccctttaa atcgataaat
    45661 tgtgttggag tcatctcgta tttttcaatt acagaattaa agttgtattc ttcaacttct
    45721 ccaactcctt tttttgttat aagagtagtt gtattgtctg aagcaagttg aatagcatct
    45781 ttgtctccag ttactatgta taccttatat ccattttctt ctgctttttt agaaacagta
    45841 cctataatat catctgcctc atagccatct atttccaatc tatttattct aaatttatct
    45901 aaaaggtctt ttaatggctg taattgttct gccagttcat ctggcattcc ctttcttcca
    45961 gccttatact ctttatattc taaatgtcta aatgtaggtg cttttctgtc aaatgctaca
    46021 cttatatgtg ttggtttata tatatctatc attttaaata gcattgttgt aaatccatat
    46081 attgcattgg tctttagccc ttctttatta ttcatttctg gaagagcata aaaagctcta
    46141 tttattattg aatttccatc tattataatt aatttttttt ccaaattata tcactcctaa
    46201 aaattatcta tttttgtaac atatatatta tataccaata tactgttatt taatattccc
    46261 ataataataa tagtatatta aaattaaata tgttgcaaac aatatatttt tttgctataa
    46321 taatatacat gacatgcccg agtggtggaa ttggtagacg cagtggactc aaaatccacc
    46381 ggactaatac tccgtgccgg ttcgagtccg gcctcgggca ccattaaaaa ctcctaaagc
    46441 tatttattca atagattagg agttttttta tttttgattt aaattttaag ttattttttg
    46501 aaattactgt gtctttttaa aatttatata attatataaa tttatcttct agcattaatt
    46561 taaaaactaa tttattttta tataattttc tattttatat cataacttat tacaactttt
    46621 tatctaattt attgtataca acaacactac aaataaattg aaataaaata agcaatccaa
    46681 gaacaactaa aacccacttg cttattacca ttacagaaaa tacactcaat atagaaacaa
    46741 taaatataag aattacaaga acataggaca tgatttgatt agtgatacgt ccagctttat
    46801 cacgtaacat aattcgtctt tcatcattca tctctatcct ttgatttttt atcttttctt
    46861 cataatctac tgctctttca ggtttactcc aatacatata ttgaagaatc atacatacac
    46921 ctggacctaa agcagctcca gataatcccc aaataaaagc ctcaaatgaa aactcagtaa
    46981 atgtagccaa taaaacaagt aaaattccac aaataaaata tataattcca acatataaca
    47041 tattcttttt catttttact cctcctcata aataaatatt tcttcaatac tcatatcaaa
    47101 gtatctagct atcttaaagg ctaagatgat agaaggatta tacctaccat tttctaatga
    47161 acctatagtt tgtcttgata cttgcaaaat agaagcaagt tcttcttgtt ttatgcctct
    47221 ttcttttctt atttcttcta atctattttt cacatctatc cccctttaat atctcaacta
    47281 cggaaagtct actttccata attatacaac tatattccat ttatgtaaag cttactttcc
    47341 atatttacaa aaaaataaca gactattttc agcctgtaat tttagaattt catgttgttt
    47401 attaatttta atgttattta ttaattccat attatttatt aattttaact tggtttctga
    47461 attggaatcc aaacttctgt agtataatca ggagcattga tatcgccatt gggataaagt
    47521 tcaatatctg gtaaattagc atacacatac ccagaacttg gtaaccactc agtaataatt
    47581 cttttttgca attcctgtat tgccagagca tttggcattg gagaaacaca ttcaaatact
    47641 gcccatgtgc ctgatggtat taagtattca tgagtatctt ctaatacagg cttatctgtt
    47701 ggagcagcaa tataataatc taaaccctta ctatttgtac acacactaac tccaagcaat
    47761 ccacaaggtt cattatttat taactctgaa agtttatcaa ttgttccatt ttttatgcat
    47821 ttagcccaca acttaggaac tttctcaaag cactcttcta caatcatgtt catactttct
    47881 tttataccaa caatacgaaa ccctttcttt tctacaatac gatattgcat ttctatattt
    47941 ccttttacat aaatgcttaa tgtcatctta ggatatgctt ttaaaacaac accctttgct
    48001 ctagctgcag atggagaaat gttatgtata ttttgaaaag ctctattaaa agatgttggt
    48061 gaatcgtaac catacttctc agatagttct aaaattttga tgttactgtt ctgaagttca
    48121 aatgcagcct tagtcatacg tcttcgtcta atatattcag ataatggtac ttctgcaata
    48181 tatgaaaaca ttctttgaaa atgaaaggtt gaacaacatg caatttttgc tgcttctgca
    48241 taatccactc cactttcgag attttcttca atataagtaa tagcttgatt taatttattt
    48301 atccattcca tttgatatta cctccaagca aattatacag tttaataaaa taatatacct
    48361 ctctattcat gcacaaaatt gtaagcattt aaatttataa gcatttttca atcaatacaa
    48421 taaatataaa aattgtatta tacaataaaa aagttcaata acattatatt ataaaatgtt
    48481 attgaacttt ttataaaaat taatttaagt gtagttaaat taagtattac actatcttat
    48541 atattacaat atcctactat cttatagtct tctggcactt tataatcttt gtctggagta
    48601 cctaaattcc attttaaatc aaacatagtt gcacttatta ttgttgcaaa ataaagcata
    48661 actataccaa tgtctatttt ttcttcatat aaattagtat gaccatcttt tctaacagct
    48721 aaaactacag tgcctccatc aactataaat ctccatggtt gtctatttaa tgtagatgga
    48781 gctaatcttg catagtgaaa tgcatcaaga agtgctcttt cttctaagat ttctatactt
    48841 gcaacatttc cccattcgtt catataaaca acatcttcta cacctagtct agtagacccc
    48901 ttatctgtta caacttgcat atcagatttt gaataatttt gcccagtttt tgtagcattt
    48961 aatactttag cattacttac attttctcca tatccaatag ctataattcc tgttacctct
    49021 ttatctgaat taattccaag tttttctttt accttttttc catcttcaaa agtaacccaa
    49081 caagaaccaa ttccaagttc tgttacttta aagattatac tttctcctat gtaccctgaa
    49141 ttttcaatgt aattatcttt tatgtcagat aatattataa tataacttgg agaatctatc
    49201 atatttcctt tgtaccctgc tactttatct aaattattaa aaacttcact tctattcata
    49261 attcttacat caacatctat ttctggaact agtttcttac aagattctga gtatttctta
    49321 atttcactaa gatattcttc tttaatatct gtgttcttat attctctaac agactttcta
    49381 tttaatatga ttgttttata atccataatt ttatcctccc caaactagta ttgtcttaat
    49441 tatataccca ttaatatatt attttactca tttatttaat aaataaagat tacaattttg
    49501 taattattat ttattaataa tattccactt ttacattaat aatgaccagt tgtgcaaaat
    49561 ccattgccaa ccaccaaata aaaacatatt ataaaggtaa tataaaataa caaacaaatc
    49621 ttaacaaaaa attaaacttt ttttacacga aagaaggctt cccgttgcct tctttatttt
    49681 tataaaaaaa taagctgttt ctaaaatgat ttcacatttt tgaaacagcc aagttattct
    49741 ataatgacaa ctataatttg aggatgtgag ggagagttgt cattataaat ttagggtaat
    49801 tatattatgt tttaagctag ttctttctat atttatataa tacattttat atatgaattt
    49861 tatatgagat tggtataaat gatatatgaa attacatatg tttatattat attaaatttt
    49921 attattacat tttccacaac aatactcata tgtctccaca ttatttggaa tatttacaaa
    49981 tacaaattct ttacagttct cacaatactt taatatagat atagttccac aagtgtaaca
    50041 ttcaaatatt acacaatcct cattttcctc tactatttca aactttttac tatgttcacc
    50101 acactctggg cacttgtgat tatttacatt cataaatact cctccttatt agtatagtta
    50161 catgtcaaaa acttaattaa tgtattctat attacttatt atatattcta taataaatca
    50221 tattctaata taataaaaca aattaaataa aaaaaaacca taaattttaa atttatggat
    50281 taattcttgt aatgataact ctatttgagg atgtgaggga gggttatcat t
//
LOCUS       DAYAPA010000013        49741 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.013, whole genome shotgun sequence.
ACCESSION   DAYAPA010000013 DAYAPA010000000
VERSION     DAYAPA010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 49741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49741
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.013"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            78..752
                     /locus_tag="ACGI59_000666"
     CDS             78..752
                     /locus_tag="ACGI59_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFG9738472.1"
                     /translation="MNNKILVVDDEEGIITLLKDYFEMNGYEVYTAQSGKDALKKISK
                     NPDIILLDINMPEIDGIEVCNRIRDYVSCPILFLTARIENTDKINGFRAGADDYIVKP
                     FDLDELGARVSAHLRREQRKSNQTKIKFYNDLTIDYEKRLICIYNNQISLSKKEFDIV
                     ELLSMNPGQVFDKEKIYDRVWGYNSNGNSDVIMEHIRKIRIKLSKHTLENYIETVWGV
                     GYKWIK"
     gene            740..1900
                     /locus_tag="ACGI59_000667"
     CDS             740..1900
                     /locus_tag="ACGI59_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFG9738473.1"
                     /translation="MDKINNMSLKKAFFVLTLSSLIIASVLTTFAYILLNNLYNSIQD
                     KYLKTPINTGIITIVQNNVNQFSDYDKTLLNIINILQLVLPLIFFIGLLLLADIIFYK
                     VKLKTPIEILNKGAMEISNNNLDFCLEYNNNDELGNLCNAFEKMRSELNKNNIKMWTM
                     INDRKQLNAAFSHDLRNPLTVLKGYSNYLTKYIPTGKLSDEKILSTTQLMSEHINRIE
                     YYVENMSNAQRLEDLVVSKSMSNINKFIENLDENISIIAKQEGKSFTLKSQINNINLF
                     FDENIILRVVENIISNAFRYARNNVSILIYLEQELLTFVIEDDGIGFSKESLKLALRP
                     FYRDKTLNDSNAHFGMGLYISKILCEKHGGSISIENNSTNSAKITAKFSTRN"
     gene            2137..2643
                     /locus_tag="ACGI59_000668"
     CDS             2137..2643
                     /locus_tag="ACGI59_000668"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFG9738474.1"
                     /translation="MTFSRIVFALLMLSSTFSSLPFWICYFWCGISDILDGLIARKLR
                     QQSGIGAKLDSIADFIFAVTILVTVGKNIALPMYLWWCIVAIALLRLISYSIGFFKYR
                     TFSSLHTLMNKITGISIFAFPLLYICFGLDVAGIMICIVAFLSSFEEIAIMIKSKELN
                     RDIKSIFM"
     gene            2929..3597
                     /locus_tag="ACGI59_000669"
     CDS             2929..3597
                     /locus_tag="ACGI59_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9738475.1"
                     /translation="MIIKAKQLSKIYGSNNNKVIALNNVNLEINSGEFVSVIGPSGSG
                     KSTLLHILSGLDNPTSGQVLLDDKDIYKHTEKELSALRRKSFGFVFQQFNLLPVLTAS
                     ENISMPVLLDKKQPDKDYLNEISSLLGIADRLNHLPHELSGGQQQRVAIARALIAKPD
                     IIFADEPTGNLDSKSGSEVMNLLIKTSKQFGKTLVVITHDDRIAKLADRKISIIDGVL
                     MEVK"
     gene            3600..6068
                     /locus_tag="ACGI59_000670"
     CDS             3600..6068
                     /locus_tag="ACGI59_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9738476.1"
                     /translation="MKNYLSLSIKELKSQKFMTTFIIIAIVLSTIMTTVVGQSIGILQ
                     NLRIEQARSFNGDRHVSFHQLTKNQVDDIKKDDRVYQAGTSITIGSSKIKDSGISVLV
                     KEYDKTGLSNYPKLMKLKSGHLPKDKNEIALDENTLKLMGIKPRLGVTIPMNLDISLL
                     NDTIPPYNYTANFKLSGILEDDYTGYVSGIVNGIVGKGTSENLLPKRYILSSLDFKIK
                     QQEKFQEIVNQLAKKINLSHNSIQYNWIYLNALKIQFEKDENSSNSDGISMIILVSLF
                     VALLVLLASGLVIYNILKISVTKKIKEYGCLRAIGAEPNQIYKIVILQILILCTVAIP
                     IGAVIGIISSKGITGMVTNILNPDILLANDNKEITELIHKNTTAYIFPLVLSTNVSLI
                     FSFISALPSAIYASHVSPKIAMVGSTTKIKRKIKREKTIKNFERHLAWLNLKRNKGRT
                     IITILSLFMSITVFVALSEFSNVLDVSRSVSNLKEGDFSLTNEISGFDKSTLDKIAKM
                     KNVNRTSFIKYSEYKQGEIDTDINFENSGEMLKIIGIDEQTLKDLMPSITDSILEDFK
                     NGSICFIKNPIAISTPGVKTRYTNLKPKDSITINKKQLDIYSTVDKMFFLQGNGWING
                     VDVIVYDSVYNTLTNKNKFNQINIYAKDKSKLEQIRLSIEQICENNPGSHWISYIESD
                     KQLKESFKQIEFLAWAVILFVGLIGTLNIINTTHTNINTRTNEIGVKRAIGMSNRSLY
                     KMFLWEGVYYGIFAAIFGSIAGYASAIIINMATIEKLDFTNIPITSILQATIISVLAC
                     IIATLIPLRKVKKMNIIDCIDINL"
     gene            complement(6308..7732)
                     /locus_tag="ACGI59_000671"
     CDS             complement(6308..7732)
                     /locus_tag="ACGI59_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HFG9738477.1"
                     /translation="MKNQSLITQFRHTFIFIIIASIVATVITYVFALYLYIHSLNKDI
                     YPPNYYERQVPRIEKYINEKNIALLSQSNEEGLKRTIRGDDMLYQVVDNNGNILYGTN
                     PKKLFKTKEELFNNFINKTVRKGGYIHTVPIKGDNGKIEGAVILFYQVKITFANIRGR
                     FVFAVIIMALFSPFLYIVGFTRWLSKRFVKNINQPLHLLIDASKKIKEKDLDFEIDYY
                     SDNELGKLCSAFSEMKDELKGSLSAQWKMEQERVEMVEALAHDLKSPLSIILGYTDAL
                     IGNNTDDNEKLHRYLTVIRENTEKSAALVQKMQYTSDLEKSNIQLNLVPINLPEFLRQ
                     KVQDYELQAHQKEVELILKMQGNIQSPIQIDVDRLTRIFDNIISNSLQYTPSGGNISI
                     TVKDEKNCISYEICDSGRGFSSKDLKKALDKFYRGDEARQTKGGHSGLGLYIVKQLVE
                     QLGGSVKIENSKSGGACVKFWHSI"
     gene            complement(7751..8443)
                     /locus_tag="ACGI59_000672"
     CDS             complement(7751..8443)
                     /locus_tag="ACGI59_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFG9738478.1"
                     /translation="MSKILIIDDEMDLVMLLEDELKAKGHEVLIAYDGQAGIELSKQE
                     PDLIILDIMMPKMNGFEVCQAIRDDVLCPIIFLSAKQSETDKIKGLTFGGDDYVTKPF
                     GLRELMARIEANLRREKRSQYINEENKRSTLYFGKLCLYMKERAIKIDGKNIDLTKIE
                     YDIVELLALHAGQVFSREQIYEKVWGCDSEGDSSTVVERVKKIRAKFSAVTPEKEYIS
                     TVWGIGYKWNKM"
     gene            complement(8495..9274)
                     /locus_tag="ACGI59_000673"
     CDS             complement(8495..9274)
                     /locus_tag="ACGI59_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutG family
                     permease subunit"
                     /protein_id="HFG9738479.1"
                     /translation="MIKLLSSEWLRAKRTAVQWLTLCMPIIFSLCVVFYLTTKSGSTQ
                     EFAFEGFFTVWTVFIIPIGVGILAGFIVQEEELAGNFNGFLCVGISRVRLYLGKFLFL
                     LFCLTICTFIATLILCIGMSIAVPSGATIWLFLSAAGLVVIGTLPLLAIHLWVSFAWG
                     MGASIGISIGGILMAAILGLTSVGEKVWAFVPWTWPVKLGMLPGIYFIKEAGTISTEA
                     FYSKVMQTASIELIVVAIGLIIFLIGGVIWFKMWEGRKSYE"
     gene            complement(9271..10041)
                     /locus_tag="ACGI59_000674"
     CDS             complement(9271..10041)
                     /locus_tag="ACGI59_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HFG9738480.1"
                     /translation="MNALQSELLKYKRTFMGKLIVFFPVSFAAYAFIMQSTLMQNPLS
                     QTTSWAWQNLLALIFNWWSFLFLPIGFALFATLVAFQEKKAGNYRALRTHNVSPMTLW
                     INKVIAMAVYSFISTLVLIVVTIITGLILKAGPVPFGQIIGASIVCWVVSLAILPLQL
                     WLATWKGMFLSMGVGALGMIFGVLAATKPFWIAVPWSWAVRMVCPIINIHPNGTILEA
                     GDPLLNTSVIPIGIVVSLVVFIVLTALTAAWFNRRDDK"
     gene            complement(10038..10742)
                     /locus_tag="ACGI59_000675"
     CDS             complement(10038..10742)
                     /locus_tag="ACGI59_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic protection ABC transporter
                     ATP-binding protein"
                     /protein_id="HFG9738481.1"
                     /translation="MKDLILETKNLSKRYGEQMAVNNVSLQIERNSIYGLLGPNGAGK
                     STTLKMLVGLLRPTNGRIIFDRKPWKRESLAKIGSLIESPALYGNLTAEENLLVHTKI
                     LGIPKDKIYEVLEIVNLKNTGKKRASQFSMGMKQRLGIAIALLSDPELLILDEPTNGL
                     DPFGIQELRELIASFPKKGITVILSSHILSEVAQVVDNIGIINGGRLLFQGMPDPNEN
                     LEEFFTDVILKGGQNR"
     gene            complement(10997..11509)
                     /locus_tag="ACGI59_000676"
     CDS             complement(10997..11509)
                     /locus_tag="ACGI59_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9738482.1"
                     /translation="MKKISQEKISTHIQEIPQEKIEQWKSLYEKYKSILKVNSKSVLE
                     IIEFLKRKYVIVEETSEEFKHVVINNIKLNKPFSQKISSDKELKPIVFTIPNEGNAKK
                     LYDTQEEIYSDCKIIVGMEFETGCVFVEGSDELNDEITSFKGLDKYDINNYYLVANYI
                     RCLKKYKLID"
     gene            11835..12092
                     /locus_tag="ACGI59_000677"
     CDS             11835..12092
                     /locus_tag="ACGI59_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9738483.1"
                     /translation="MILNRAELFRGYSLNRFNEICNILSVNKIKYKYKVKNNAYSKKP
                     FFNEITLGNLGQKEDFSYEYLIYVHKDDYEQAESLINSKLR"
     gene            12235..12861
                     /locus_tag="ACGI59_000678"
     CDS             12235..12861
                     /locus_tag="ACGI59_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HFG9738484.1"
                     /translation="MKINIKSKLSEFIKQNNLFDDSRIISYLPNITIETDKIKTIMIN
                     EVVPTNTNDDFYSVDKDADYLKTTIPLFDSAGIKVSNINDILDMGIYITNAVKLPKSE
                     YTITRDTIKLHMPILEEEIKLFKNLEVVMLMGDVAKKSFNMITKKHIKKNVIPSISTY
                     KLRNNELYYDNLRVFPSYIMTGGNILIEKSKFEMASEDIKKMFEIING"
     gene            complement(13108..13887)
                     /locus_tag="ACGI59_000679"
     CDS             complement(13108..13887)
                     /locus_tag="ACGI59_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901940.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HFG9738485.1"
                     /translation="MNSKIYYFSGTGNSLVVSNILKETLNGSKEVIPLAIHRKENNID
                     INEDILVIVFPVYFADVPDIVKSFVEKLNFNINTHIYAIATCNGVAGHSLFTIDKLLK
                     KRGKKLSAGFLINMPGNALITPPDVEEERLKNQTNRVAEIAKCINNLEIGNIEGKNNL
                     KSHIDSVVLRLVGKNMQIATTKFFAENKCNGCGTCKRVCPVENISIVDKKPKWGNECE
                     RCLACFHWCPKEAINVKKSFLTNRKKYHHPDITVKDIFLDK"
     gene            complement(13955..14416)
                     /locus_tag="ACGI59_000680"
     CDS             complement(13955..14416)
                     /locus_tag="ACGI59_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFG9738486.1"
                     /translation="MGGARDYLNGGKMKDFEKLYEATTKLVSTSLKVKDDLKKMFKQN
                     GYDITTDHYALLRFLWEQDGISQIDLCEKSCKDKSNTTRILDVMKNKGLIVRKVDVKD
                     RRKFQIFLTDLGRELEEPLNEIASIYATETFKSISDEELPLFTDILDRIGK"
     gene            complement(14392..15069)
                     /locus_tag="ACGI59_000681"
     CDS             complement(14392..15069)
                     /locus_tag="ACGI59_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HFG9738487.1"
                     /translation="MIITVINGSPRKNGATSKVLTYLYKDIERLIPDVKINYFDLSEV
                     NPSYCIGCLNCYKMGKCINQNDKVEYIHDIITKSDGVIFGSPTYGSSVTGLFKVFTDR
                     AHMMLERLLYRKPCIAVTTYENARGSKAISFIKSMVLDSGGYVCGSLSIKTGFNQNPI
                     TEKVESKIQKVSKKFIYCIEEKKNPPVLSQIYNFIAINAVLKPMAFKDIEQYKGIIDR
                     WEEQGII"
     gene            15302..15688
                     /locus_tag="ACGI59_000682"
     CDS             15302..15688
                     /locus_tag="ACGI59_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HFG9738488.1"
                     /translation="MKKLPHISEAEYQVMKIIWKYAPISTTEVIEKLVETSTWSPKTI
                     QTMLLRLVKKGALTYEKNSRVFVYTPLVKEEEYVATESSSFLNRFYNGTLNSMVLNFL
                     ENDKLSEDDIEELREILNKRTTKGDK"
     gene            15691..17490
                     /locus_tag="ACGI59_000683"
     CDS             15691..17490
                     /locus_tag="ACGI59_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaR1 family beta-lactam sensor/signal
                     transducer"
                     /protein_id="HFG9738489.1"
                     /translation="MYAPFFIRFLLSTLTLSILIAVILLAKKVFKKHISAKYQYNIGF
                     ILLIMLIIPFVPLKYINLGNVFNYLYTFNQLGSSTNRTSMIKNSPNSIINNSNALNDF
                     SVSLNQVNFDSLNILLLISWIIGILIMIAITIHCNIKIKNMKKSIQILRDEEIVFIFE
                     KCKKDIGINKDFILCKSPIINTPITFGYFKPHIILPDKSISKLSLKDIKYILLHELQH
                     FKNKDILINYIMCFLQILYWFNPLVWYAFKEMRIDREIACDISVLKKLDKDSHIEYGQ
                     TLINFVDNISRPSNLTLTSDIGGSKKQIKKRLLKIMDFHNESNTLKFKSILIFTLIAL
                     IVFGNIPKLSIMADINEKYRPTNEQTVYEDLSQYFKGFDGSFVMYDLKSKQYHIYNKE
                     KSKTRISPNSTYKIYSALLGLENGIITRNNSHIPWDGKHHSNSSWNEDQDLFSAMENS
                     VNWYFQTLDKKTGLKNIQTYLRQIGYGNYDVSGGISNFWLESSLKISPIEQVELLESF
                     YTNKFKFEDKNIETVKDSLLISKKDGVSLFGKTGTANINGKDVNGWFIGYVEKNENTY
                     FFATNIQSKDYSNGSSASKITLSILNDKKIYNP"
     gene            complement(17845..19719)
                     /locus_tag="ACGI59_000684"
     CDS             complement(17845..19719)
                     /locus_tag="ACGI59_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFG9738490.1"
                     /translation="MNKYNKIANELIKIIGEDNIISITHCATRLRVMVKDREIINDKK
                     VEKVDEVKGVFFTSGQYQIILGTGIVNKVYAEVEKMGLKTLSKKEQDELVKNNETGFK
                     KVMRTLADIFVPIIPVIAATGLFLGLKGCLFNDNVLGLFGMSSANIPLYIQTLVSVLT
                     ETAFAFLPAIIVWSAFKVFGGTPVIGLVIGLMLVSPILPNAYSVADPSNEIEAIMAFG
                     FIPIVGCQGSVLTAIVTAFIGANLEKWFRKHMPNVLDLIFTPFFVMLITMLVILLGVG
                     PIMHTIELKMVDIISLLIDLPLGIGGFIIGFTYPLAVITGLHHTYVMIETSLLANTGF
                     NALITLCAMYGFANIGTCLAFMKKSKNNQVKQTAVGAMLSQLFGISEPVLFGIQLRYN
                     LKPLIIMCASSGLGAAILSILHIQSNSYGLAVLPSYLMYIYDGYNLITYLLASIFVVA
                     FCFIVTCLFGVPKEAINEDEDEELVFNENNENFVSPAKGKIVALENVPDETFSKKMLG
                     DGFAIDIIDGKIVSPISGKLETVVSSGHAFGIKGTNGEVLIHVGIDTVALNGDGFDIA
                     VKQGDMVKQGDVLVNVDLKRIHELGKSTLTMVLFPDGKKVNILDINKDVKIGQRICVE
                     "
     gene            complement(19724..21388)
                     /locus_tag="ACGI59_000685"
     CDS             complement(19724..21388)
                     /locus_tag="ACGI59_000685"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0004556 - alpha-amylase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFG9738491.1"
                     /translation="MEKKWWQKEIVYQIYPRSFQDSNNDGIGDLQGIISRLDYLKDLG
                     ITSLWLCPIFKSPMDDNGYDISDYLDIASEFGSMEDLKELIKKSKDRGIKIILDLVLN
                     HTSDEHPWFQEALKNPESPYHDYYIFKKGNDLPNNWRSVFGGSVWEKVENRDEYYFHS
                     FGKKQPDLNWENPILRSELYQMINTWSQMGVAGFRVDAITFIKKNLSFQSLESDGVDG
                     LVKCTKTVRNQPGIEKMLYELKENTFKKYDCMTVAEAAGISYDQLGDFIGEDGFFSMV
                     FDFKHADLDVASGSEWFKRISWTIPDLRKCILDSQMYVQEHGWAANFIENHDQPRATT
                     KYLLQYERNDDAVKMLGAMYFFLRGTPFIYQGQELGMTNFKRDTINDFNDLSSIDQYY
                     RSIDEGYSEEEALEFVNLRSRDNARTPFPWADKENAGFSDTTPWLKVIENYKDINAQS
                     QINNSESILEFYKKMIKLRQDSEYSDCLIYGSIEPIDIESDDIIAYRRQLDLVVIDCY
                     YNFSDKPVEIRVEEKYDIVFQNLDDINLLNKTVRLKPFHAVLLKVR"
     gene            complement(21402..22223)
                     /locus_tag="ACGI59_000686"
     CDS             complement(21402..22223)
                     /locus_tag="ACGI59_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFG9738492.1"
                     /translation="MKLYKLLILDIDGTLRDEVYGIPESAKHAIRLCQKNHCSVVICT
                     GRSMGTIQDDVLSLGVDGYIAGGGNYIQYHGELLYNQSFNQRLIKEVVCLLKNREVAF
                     SIESQEKVFMNQKAKEIFETMNQLKGTNSCINKQHIQEKITYENNIEEYKSQDIHKIC
                     LWSNEKVFDEVKDILQDKMELAQRDISSQYYEIIQKDFHKGKAIKRLQERLGVTQKET
                     ICFGDGQNDIVMFQASDVTIAMKNSHQQLKDIATSICEDIFDNGIYKELKRRNII"
     gene            complement(22322..23086)
                     /locus_tag="ACGI59_000687"
     CDS             complement(22322..23086)
                     /locus_tag="ACGI59_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFG9738493.1"
                     /translation="MKIEGLFNKYYNSFTANDKYICECILNHKEDCIKLSIDEFADKY
                     HISTSALSRFAQKLQLPGYSELRVIIRLNETEVEYKNQSKHQLMDCYNKVINDIEKKD
                     CSIMFERMKKANRIIVFGEGYSQERVAKEMKRIFLPTGKIIYDVYGYDMIQALNHFIK
                     TDDMIFFISLNGDASTIIDFAKEMRMKGIYMVSITKMISNPLARLCDENLYIQSISIS
                     VDKQLDYDVTTPYFILIELLYIKYKMYLESEHIPRK"
     gene            complement(23083..23832)
                     /locus_tag="ACGI59_000688"
     CDS             complement(23083..23832)
                     /locus_tag="ACGI59_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFG9738494.1"
                     /translation="MRLESLVNQNYDNLNENDKIIIKQILICQREYRNFSCEKIANEC
                     NVSRATLLRLCRKIGLDSFSDLKYLLKNNQLEFVNNDNNFHDVCENYRLLMNELQKIS
                     FQDICKIIYDAETIYIYGTGNEQKSLAQEIKRIFLSVGKCVIDLFDIGEIEFMRESFQ
                     KSDLFIIISLSGETKAGIEIAKFINNHIHTISLTRLDNNTIATLCEYNLYVETQKLDT
                     LQAISYEVVGAFYALLDLLHMNYIEYRRRKE"
     gene            complement(24336..24941)
                     /locus_tag="ACGI59_000689"
     CDS             complement(24336..24941)
                     /locus_tag="ACGI59_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFG9738495.1"
                     /translation="MKIKENVLMLDSTRGSNCFIVFDKEIILIDTGLPFMGKNIIKEL
                     KTLGIKLTDIKHILLTHHDVDHISNIKLLKEKTGAKVWAHVEDIPFIIGEKDRPGFKK
                     FIGKAISRRLIKDVEPYGENMQIGNIKIIHTPGHTPGHVCMIFEDVLFAGDLVKNKNG
                     NIVPYPNPWNWDYKKMMKSIRELDNLKYEWICMSHGTPCIK"
     gene            25120..25758
                     /locus_tag="ACGI59_000690"
     CDS             25120..25758
                     /locus_tag="ACGI59_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFG9738496.1"
                     /translation="MSELSKRDKEKIQRENEIIDKAEKLFCLNGFDNTTMNELAKEVE
                     YTKRTIYKYFSCKEDLFFAVVLRGYKRLWDNVKIESAKGKTGFEKIKLSYFAFHKFYS
                     VEPSLLCLMGMIGIVNSEKSDTDMLFKEKFFSFNKFMFDEIQGMFEIGKNDRSIRHDI
                     EIPTLMYSSIFTLTGFFNLLSVTGKSYLNNFNIDEEKFIETTLALLIDSIKA"
     gene            25890..26552
                     /locus_tag="ACGI59_000691"
     CDS             25890..26552
                     /locus_tag="ACGI59_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFG9738497.1"
                     /translation="MAKILLLEDNDTITFGIKTSLEKKNHTVCCYNTIAEAKKNFDKT
                     IDLILLDLNLPDGIGYEFCQYVKSIEDTPVIFLTVRDDEKDIIRGLDMGADDYVVKPF
                     LLSVLQSRINAVLRRTHSNTNNTITCENISLNKVEVKAYLDNVEVPLTAGEYKLLLSL
                     MENKNRAMTRTKLLEMLWDVDGNFVNDNTLTVTIKRLREKLKNPPCIKTLRGIGYRME
                     DN"
     gene            26552..27733
                     /locus_tag="ACGI59_000692"
     CDS             26552..27733
                     /locus_tag="ACGI59_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFG9738498.1"
                     /translation="MKTPRNINSTVVFIIFCLIVTFLLSVILTTITSFNEYNVLSKAL
                     GASYEYNPKSTESILKSLKNSSDKDFKTGNEILSKHGYSKSTFWSKNFFYFLVISFGI
                     ISTMATVVYIIKHRTNKQKLSRINNLTQYLEEVNLGKESVLLRCEDEFSHLEDEIYKT
                     VGELRCSKEFALKERQTLSDNLADIAHQLKTPITSMSLMAQLLSENCPDEEIDYINRL
                     TNQISRLERLVSSLLTLSKLDASTLIFESKLIDVHSLLTYAIEPIEGSLREKHQTFTI
                     EASPSVEFEIDINWTLEALLNILKNCSEHIEDGGYIVAYYSQNPLYVEIIIEDNGKGF
                     AQEELPYIFNRFYRGKNASKDSIGIGLALSKSIIERQNGTLHAENCHNGGARFIIKFY
                     T"
     gene            complement(27914..30574)
                     /locus_tag="ACGI59_000693"
     CDS             complement(27914..30574)
                     /locus_tag="ACGI59_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFG9738499.1"
                     /translation="MNILQEYTLDFVRRNKRSSIAIMLAILLTTTMMSSLCGLLYTMW
                     TDFIKLSIEKEGNWHGELFDYTYGSDLPLIENFSSVDDVMVKGQWYVGKINDEKRDYI
                     IYRNATSEYWNSMPEKDTIIEGRRPNNANEIALSKQYFENNPSVKIGDKITLPIGDRI
                     LNGKPLEPIAPKSKGESFKKISEATLTIVGKVDVSTSSVIPAYTALGYLDKTSIKPND
                     SITVYLRFKNIRDTYKELPKLAKSLGWKTNEYGKYNLKYNSNYLLKMLVFSPEQKASM
                     SSISKFSTPIMYLTIAIFTVAVFVMVIYNAFSLSANARLTQLGILSSVGASPKQIKRS
                     VVFEGFLLTIIPLPIGLFLGWLLCNRLIVYVNSVNYHTDVPEVVFTYGIPAFLPAVLL
                     TIVTVWISALIPARKVSKISPIEAIRQGDSVKIKKSHKYSLGKIFGIEGELASNALRA
                     RKKSYRTATISLTLSFLLLTCFLHIIANQEGAKAVYGSDNYKDQRDIAVYLENSEPVE
                     SAFIEKLDNTEGVKSSLYYQKMSASTWLTEKDASKELAKNGGFKNIASSKKYSPIERD
                     GKFRIISNIIGLDDKSFTEYCRQISVDPKLFYDTKNPTSIVYNKEEDINRSTRRNKVY
                     IDYLNLSIGDNIKLNEKVYDEDKGDYTYNMKVGAITSTLPKIVDTSSKYTLMQIVPMS
                     MVQEISKNYDEVSRLRSNRLIGVFKVNNREDIPRVREKLESIYNKDYGSGDYSIEDVL
                     ESEKQKASGIKTMNLIVGFITGLLAMIGLSNVLATVSGNIRSRRQEFAMLRSVGLSPE
                     GIKKMLILEGLFLGITPLLLSIPVQIGIVYAFLRINEIYFIEYLPFAPISTIIGFTIL
                     ILFIVIASYMTGYKQLKNENIVESIKNETI"
     gene            complement(30571..31254)
                     /locus_tag="ACGI59_000694"
     CDS             complement(30571..31254)
                     /locus_tag="ACGI59_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9738500.1"
                     /translation="MEILKCENLTKIYGSNQTRVTALNNVNLSVQKGDFVSIVGASGS
                     GKSTLLHLLGGVDRPTSGKIYVEDTEISSLKEEALAVFRRRKVGLIYQFYNLIPTLDV
                     RKNILLPMLLDKRKVDEDRFSEIVSILGLSDRLNHLPSQLSGGQQQRVSIARSLIYRP
                     AILLADEPTGNLDRKNSEEIVDLLNLSNKRFNQTILLITHDEKIALEANRIVTMEDGV
                     IVSEKVVKK"
     gene            complement(31598..32551)
                     /gene="blaCDD"
                     /locus_tag="ACGI59_000695"
     CDS             complement(31598..32551)
                     /gene="blaCDD"
                     /locus_tag="ACGI59_000695"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDD family class D beta-lactamase"
                     /protein_id="HFG9738501.1"
                     /translation="MKRKKNFIWIAILLVGVVMVMYYVGKKHNDINQKTDKNYIKSEL
                     NKSELNKSKIENNNKNKKEKINMVDYSDCFEGISGGAIFYNTKNKEYNIYNKELIETR
                     RSPCSTFKIVSTLIGLEKGVINSKESVMGYDGTEYPNKNWNKNLSLEEAFKESCVWYY
                     KKLIDKVDAKSVQNILDDLKYGNCDISEWEGDLKNGKGHLNGFWLESSLQISPKEQVQ
                     TMAKIFEGDTNFKKEHINILRDIMKIDVNDKNINVYGKTGTGFDEKNKRVDAWFVGML
                     EREGDTYYFAIKSDDSNKEITGPKVKEIAINIIKKYYSVRE"
     gene            complement(33092..35206)
                     /locus_tag="ACGI59_000696"
     CDS             complement(33092..35206)
                     /locus_tag="ACGI59_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFG9738502.1"
                     /translation="MSENKQVVNCDIIASNSKHNMCEYDAELLTFFPDEIAHLIEINN
                     KLDNAFKKAENLVDKLDKDYMDAKMYMVKNRGEIDPHEMFQNEQGLKQIDNYGAFMVK
                     VRDKINKIKDSPYFARIDFRLKDMDDESKYYIGRFAFDYEDELIILDWRSPIASMFYD
                     YEIGKAGYDAPIGWVDGEITRKRQFKIKNGKLEYALESSINIQDDILQKELSHTSDEK
                     MKSIISTIQKEQNQIIRNDKADTLIIQGVAGSGKTSIALHRIAFLLYRFKDKISANNV
                     IILSPNKVFGDYISNVLPELGEEPLCELSFENIAEVQLDRVINFESEKDPLEINDAKW
                     SERVRFKSTLDFVKLIDDYIKQMPNKIFIPKDYTFGSFTAKSDWIQSRFEAYNRYPVK
                     KRLEKVAEDIHYKFESDNIMEEDLPKVKSILKSLNGMLTIKNTLTLYKDFFKQMNISN
                     MFVMATKKTLEWSDVYPFIYIHAAYEGIQEDKIIRHVVIDEMQDYTPIQYAVINLLFK
                     CKKTILGDFGQLVNPNHTHTLDDMKQLYNDGELVMLNKSYRSTFEIINFAKKIQDISS
                     LEPIERHGEEPALLKCNNEQDEINKIKIEIEEFKKSDNATLGIILKTDNDAKAVYDAL
                     REKYSVHLISSESSSFTKGVSITSIKMSKGLEFDEVIVPSVNNKTYYSDYDRSLLYIA
                     CTRAMHKLKLTYTGELTQLIDM"
     gene            complement(35464..36324)
                     /locus_tag="ACGI59_000697"
     CDS             complement(35464..36324)
                     /locus_tag="ACGI59_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFG9738503.1"
                     /translation="MSYKVINVITAIDYIEEHLSEKLDLDIVANAVHYSKYHLHRTFT
                     TSVGLTMHDYIKRRKLTEAAKLLVFSKKPIIEIALIAGYESQQAFTSIFKAMYKKSPN
                     KYRKEQEFYPLQLRFVLKKDNLFSKNVLELEKEIKLASMSDIPLWMNLVRLVIDGFPN
                     LQEEEYIYQLEQYILEERALILKLNNVAIANMVFNRETRSIDFFGIHPQYRNSDIAQV
                     FLKKVIEDFLIDTDISITTFREGDKADTGHRDMIKKLGFAEAELLVEFGYPTQKFILP
                     CVEERDNRQN"
     gene            complement(36919..37281)
                     /locus_tag="ACGI59_000698"
     CDS             complement(36919..37281)
                     /locus_tag="ACGI59_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFG9738504.1"
                     /translation="MKIYDILTRFYIQDIEKAIPFYENLLKEKCSLRFSYKEVGLELA
                     QIGNVLLLSGSDDALKPFIETKSTFMVDSVDEWRSYLLDNGAVVVRDKKKVPTGYNMT
                     LRHPDGTIIEYVQHTKQD"
     gene            complement(37515..37979)
                     /locus_tag="ACGI59_000699"
     CDS             complement(37515..37979)
                     /locus_tag="ACGI59_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFG9738505.1"
                     /translation="MTRISNITIAEQSEYCFLAIRKTIDFMVEFSEFSKQSFEKISKY
                     LEERGILSSGAPIVCFHNMDLAKLDVEVGFPVATYIDGKNEILQRIVPAQKIITAIDL
                     GPYELQDPTLEDLFSWANSNGYKLHGDIYYQYLNDINRPASEYLTKMMLPII"
     gene            complement(38408..39829)
                     /gene="nhaC"
                     /locus_tag="ACGI59_000700"
     CDS             complement(38408..39829)
                     /gene="nhaC"
                     /locus_tag="ACGI59_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFG9738506.1"
                     /translation="MKERECRKASLFDAMIPITCLILFLSLGVLVYGSSPHVPLIGAA
                     AVAGLVAVYRLGFKWKELELSMFNSIKMAMQAILIIIIIGVLIGTWIVSGVVPCMIYW
                     GLKILSPNIFLVASTLVCAIVSLATGSSWSTMGTVGVALLGIGQSLGMPIGLIVGSII
                     SGAYFGDKLSPLSETTNLAPAMAGTDLFTHIKYMLYSTIPSLLICLVIYGVIGMKYSG
                     QALDIKQIELIRSTLDSTFNTLSPVLLLAPAIVIGLVVFKVPAIPGLIVGAVLGVLFA
                     VVFQGESMNTILDAAQNGYVSSTQIQEVDALLSKGGIMNMMSTVSLTICALSLGGILE
                     KTGMLEVVASSLLRLAKGVFGTVLCTMVTCTITNIIAGEQYLSIVIPGRMYNKEYKKR
                     GIHPKMLSRALEDSGTLTSPLVPWNTCGAYITATLGVSALTYGPYALLNIINPIVSLV
                     LIACKFKIARIEDEPETCLNFDV"
     gene            complement(39859..40923)
                     /gene="orr"
                     /locus_tag="ACGI59_000701"
     CDS             complement(39859..40923)
                     /gene="orr"
                     /locus_tag="ACGI59_000701"
                     /EC_number="5.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine racemase Orr"
                     /protein_id="HFG9738507.1"
                     /translation="MYPRLEIDIEKLKHNAKLISQMCHERNIKISFVTKSFCAQKEIV
                     EEICSEGIDHIADSRIQNLKNLQDINLPKILIRIPMLSELEEVINYCDISFNSELGTI
                     KKLNELCESKNIIHKIVLMFDLGDLREGYFYEEDFFSNVREIVRLKNIEIIGIATNLT
                     CYGAIIPSRENIGRLVSIAKRMEEKFGINLEIVSGGNSSSIHLLINNNMPEGITNLRI
                     GESILLGRETAYGENINGTYQDAFKLICQVVECKEKPSVPIGEIGVDAFRNKPVYEDK
                     GILKRAIIAIGKQDINIDSLIPIDTDIKILGASSDHMILDVSNTKYDYKLGDNLEFLL
                     TYGGIMSSSTSKYVSKKIIV"
     gene            complement(40908..42317)
                     /locus_tag="ACGI59_000702"
     CDS             complement(40908..42317)
                     /locus_tag="ACGI59_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlmL-related ornithine degradation protein"
                     /protein_id="HFG9738508.1"
                     /translation="MENSKVSLKIDVLVAEIGSTTTVVNAFHDINTNNPIFLGQGQAP
                     TTVFEGGDVRNGLSGAIKDLANKLSVDDIEYNDMFATSSAAGGLKMTVHGLVYDMTVK
                     AAKEAALGAGAVIRQITSGRIKRTDLNKIKEINPNIILIAGGVDYGERDTAIYNAEMI
                     ASMNLGIPVIYAGNVENQEEIRLIFEDTNYKLYITENVYPKIDLLNIEPTRRIIQSVF
                     EEHITTAPGMKYIKEMVNENITPTPGAVMEASKLLYKNIGDLLTLDVGGATTDVHSVT
                     DGSDYINKILVNPEPTAKRSVEGDLGVYVNMKNIVEVIGKENLQSELSIDIDAVIENY
                     PPIPKSKEEILFVERLTKEAVVKAMLRHSGKIRNIYGTTGKVKIAEGKDLTEVRYIVG
                     TGGALTRLPSRIEILDNMLKYNKNNELLFPKEKTKILIDNDYIMASMGVLSKKYEEAS
                     LKLLLKSLNFEEERLCILG"
     gene            complement(42317..44521)
                     /gene="oraE"
                     /locus_tag="ACGI59_000703"
     CDS             complement(42317..44521)
                     /gene="oraE"
                     /locus_tag="ACGI59_000703"
                     /EC_number="5.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ornithine 4,5-aminomutase subunit OraE"
                     /protein_id="HFG9738509.1"
                     /translation="MLKENKKLDIDYILKDLDKYKPKRRGWVWREHLENLEMGPFKYK
                     DCTKPLKKSVGLPSSKYFNNIDPQPSPVITTEIASGRFEDDIRRMRMAAHHGADHLMV
                     IRTAGQSHFDGLIEGTPQGIGGVPITRKQVRAQRKALDFIEEEVGRPINYHSYVSGVA
                     GPEVAVMFAEEGVNGAHQDPQYNVLYRNINMVRSFVDACEAKKIMAFANIAQIDGAHN
                     ANATAREAWKVMPELMVQHALNSIFSEKIGIDKSNICLSTVPPTAPPAPCLKIDLPYA
                     VALRELFSDYKMRAQMNTKYMESSTREATVTHVLNLLTSVLTRADIQSTITPDEGRNV
                     PWHIYNIEACDTAKQALVGMDGLMDMIELKDVGELRDKARELKERAVLYMEEISEVGG
                     YFESVEQGFFVDSGNYPERNGDGISRKIKGGVGEGTVYEREEDYLAPVTAHFGYNNVA
                     QYDENAIDNPSMLIDGCTFENPDKIIYIDELDDFDNVENRLKESYEYRNGTKIKPEME
                     WCADGIVMITMMLPTDKRTAEFAALEFVKKMNLQEIEVISREVMHESEGTRIEVKGRV
                     PFDIDLNNLVIPEEPKVLTDEEIRADIEEKPMKIVSATVGEDEHSVGLREIIDIKHGG
                     IEKYGIECHYLGTSVPVEKLVDAAIELNADAILASTIISHDDIHYKNMKKLHDYCVEK
                     GIRDKVMIACGGTQVTPEIAVEQGIDAGFGRNSKGIHVATFLVEKRREMSNK"
     gene            complement(44515..44889)
                     /locus_tag="ACGI59_000704"
     CDS             complement(44515..44889)
                     /locus_tag="ACGI59_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine aminomutase subunit alpha"
                     /protein_id="HFG9738510.1"
                     /translation="MKKREDDFEVRRKHLQELSDDELKERFWSLATQIVEPMIELGKK
                     NTTPSIERSVLLRMGFSSLEVKPILEGVMERGLIGKGAGHVVYKLAKSKNITVREAGL
                     LLVKGEYWDEVTCLFREGVESC"
     gene            complement(44967..46397)
                     /gene="ortB"
                     /locus_tag="ACGI59_000705"
     CDS             complement(44967..46397)
                     /gene="ortB"
                     /locus_tag="ACGI59_000705"
                     /EC_number="2.3.1.263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-oxopentanoate thiolase subunit OrtB"
                     /protein_id="HFG9738511.1"
                     /translation="MNNSTSMKDMSYQAVMGRNNEIMKNAIGLDYSYFEQEGIGFDYE
                     KMMSETGYTLQDIEAIQSQYAVGNTPLIELKNLTKLARKCAKEGKGARIFVKDEAMNA
                     SGSFKARRAATAVYHAKQMGYKGVIAATSGNYGAAVASQAAMQGLKCIIVQECYDSNG
                     VGQPEIIEKARKCEAYGAEVVQLTVGPELFYTFLVLLEETGYFNASLYSPFGIAGVET
                     LGYELAIQFREKYKKDPDIVVCTNAGGGNLTGTARGLIKAGSINTKVVGASVDLKGLH
                     MASDNQFNKKSFTTGHTGFGIPYCTWPDRSDVPRSAARPLRYMDRYVLVKQGEVFYTT
                     ELLAQLEGIERGPAGNTSLAAAFSLAQELDEDKVIVVQETEYTGAGKHICPQLTFARE
                     NGIDIKFGNPREEIAGVNLILPERPELLKCVDIDMNKIRKSFIKNCISNNHIDNIDKL
                     SNRDIEFLMEEVKSSRDFVVDVLNNL"
     gene            complement(46390..46698)
                     /gene="ortA"
                     /locus_tag="ACGI59_000706"
     CDS             complement(46390..46698)
                     /gene="ortA"
                     /locus_tag="ACGI59_000706"
                     /EC_number="2.3.1.263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-oxopentanoate thiolase subunit OrtA"
                     /protein_id="HFG9738512.1"
                     /translation="MDAKINDWVIIHNIVLTPEERAPQVPEDTKKVSLEMWVKGFIQS
                     DASIGDLVEVKTITGRLVKGNLLKINPYYTHDYGKCIPELLQIGIQAKEILFGGVYNE
                     "
     gene            complement(46842..47909)
                     /gene="ord"
                     /locus_tag="ACGI59_000707"
     CDS             complement(46842..47909)
                     /gene="ord"
                     /locus_tag="ACGI59_000707"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,4-diaminopentanoate dehydrogenase"
                     /protein_id="HFG9738513.1"
                     /translation="MRKVRVGIWGFGAMGIGMANMILKKEGIEIVSVCSRSTSGKSMY
                     NVLGIERGERPEVIINKNYEEVFREKSVDVVLLATDSFTKKAFDKIIFLLNRKINVIS
                     TAEQMAYPQADDADLAKKMDEVAKENGVSILGTGINPGFVLDLLVLALSGTCEEVTSI
                     KAKRVNDLSPFGKSVMEEQGVGVTREEFIKGVEDKTIAGHVGFVESINMIADGLGWKL
                     DKIEQTKEPIMTTVDRKSKYGEALAGNVAGCRQCGYGYVNGEVLIEMEHPQQIIPEAE
                     GIKTGDYVSIKGIPNIDLQINPEIPGGVGTYAMIVNSIPLIINARPGLKTMLDIPVPR
                     AIMGDIRNQIEVELEEESKAN"
     gene            complement(48165..49565)
                     /locus_tag="ACGI59_000708"
     CDS             complement(48165..49565)
                     /locus_tag="ACGI59_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 interaction domain-containing protein"
                     /protein_id="HFG9738514.1"
                     /translation="MNQEIFFKIMNEILENIDEGVHFVNKDGKTIIYNNSVQKMEKMN
                     RHDILTNSFFDIVNKMKINRSTLLEVLEKREVIKDNIQKYLDKNGREITAINTTIPID
                     IKGEFVGALEISKNMTTIENLLNRNYKKNLVNKVSIRQKKGYEFDDILGESNLIKEAI
                     AKSKKACKSDASVFIYGETGTGKELFSQSIHYGSNRKNYPFIAQNCAALPESLFEGIL
                     FGTSKGGFTGAIDRPGLFEQANKGTLLLDEINSMPIMLQAKLLRVLQEGYVRRVGGTK
                     DIPIDVRIIATTNESPKVILNEHKMRKDLYYRLNIIHIKIPPLRDRKEDIPLLCKKFI
                     SKYNKKLNKKVVGISDEAIKILKNYDWQGNIRELENIIYCTMSMMEDEVQIETSMINL
                     NDYYNLKENKESNNYSLNDLEDKTLSKIISEIEEKYIYESLEKASYNITKAASILGMN
                     RQNLQYKIKKYNIKIL"
BASE COUNT        16864 a         8385 c         5910 g        18582 t
ORIGIN      
        1 agaatttgat aatttattga gatttttata ctaaaatata catactatac aaacttaatt
       61 tagaagggag cattcttatg aacaataaaa tactcgtcgt agatgatgag gaaggtataa
      121 taacactctt aaaagattac tttgaaatga atggttatga ggtttacact gctcaaagtg
      181 gaaaagacgc attaaaaaaa atatctaaaa atccagatat cattctactt gatataaaca
      241 tgcctgaaat agatggaatt gaagtttgta atagaattag agattatgta tcatgcccta
      301 ttctatttct tacagctaga attgaaaata ctgacaagat aaatggtttt cgtgctggtg
      361 ctgatgatta tattgtaaaa ccttttgatt tagacgaact tggagctaga gtatctgccc
      421 atttaagacg tgaacaaagg aagtcaaatc aaactaaaat taaattttat aatgatttaa
      481 caatagatta cgaaaaaagg ttaatttgta tatataataa tcagatttca ctttcaaaaa
      541 aggaatttga tatagtagag cttctctcta tgaatcctgg acaagtattt gataaagaaa
      601 aaatatatga tagagtctgg ggatacaata gcaatggaaa tagtgatgtt ataatggaac
      661 acattagaaa aatacgaatc aaactatcaa aacatactct tgaaaattat atagaaacag
      721 tctggggagt tggttataaa tggataaaat aaataatatg tcattaaaaa aagctttctt
      781 tgttcttact ttatcttcac taattatagc atcagtgctt actactttcg catacatttt
      841 acttaataat ctatataatt ctatacaaga taagtattta aaaacaccta taaatacagg
      901 tattataacc atagttcaaa acaatgtgaa ccaatttagt gattatgata aaacattact
      961 taatataatc aacatacttc agcttgtact tccacttatc ttttttatag gacttttatt
     1021 attagctgat ataatttttt acaaggtaaa attaaaaaca cccatagaaa tactcaataa
     1081 aggtgcaatg gaaatatcca acaacaattt agacttttgc ttagaatata ataataatga
     1141 tgaattaggt aatctatgta atgcttttga aaaaatgagg tctgaactaa ataaaaacaa
     1201 cataaagatg tggactatga ttaatgatag aaaacaactt aatgctgctt tttctcatga
     1261 tttaagaaat cccctaacag ttctaaaagg ttactcaaac tatttaacaa aatacatacc
     1321 aacaggtaag cttagtgatg agaagatttt atcaaccaca caacttatgt ctgagcatat
     1381 taacagaatt gaatactatg tagaaaatat gagtaatgct caacgattag aagatttagt
     1441 tgtttcaaaa tctatgtcga atataaacaa gtttatagaa aacttagatg aaaatatatc
     1501 tatcattgct aaacaagaag gaaaatcttt tacacttaaa agtcaaataa ataatataaa
     1561 tctatttttt gatgaaaata tcattcttag agtcgtcgaa aatataatat cgaatgcttt
     1621 tagatatgct agaaataatg tatccatact tatatatctt gaacaagaac tccttacctt
     1681 tgtaattgaa gatgatggta taggatttag taaggaatct ctaaaattag ctttaagacc
     1741 tttctataga gataagactt taaatgacag caatgcacac tttggtatgg gtctttatat
     1801 ctcaaaaata ttatgcgaaa aacatggtgg ctctatttct attgaaaata attctactaa
     1861 cagtgctaaa attactgcta aattctcaac aagaaattaa aatgattttg taagctttca
     1921 ctgttcttat ataaaatttg ttagtttaga atttataaaa tgtatttcaa tttacaacca
     1981 atatatatac tattttgtag aagttgtaag agttgcttca attataatat ttggcaaaag
     2041 gcatttgaaa taataataga aatatatatt taaatatgta gaatagtatg aaattagaat
     2101 tttggaggtg attatattaa aagttttgct aataatatta cattctctcg tattgtgttt
     2161 gcattgctaa tgcttagttc tacattctct tcactgcctt tctggatatg ttatttttgg
     2221 tgtggaatta gtgatatact tgatggtctt attgcccgaa agttgagaca acaaagtgga
     2281 ataggtgcaa agttagatag tattgctgat ttcatttttg cggttacaat tttggttact
     2341 gtcggaaaaa atattgctct tcccatgtat ctatggtggt gtattgttgc aatagcattg
     2401 ttacggttga ttagttacag tattggtttt tttaagtatc gcacattttc atcgcttcat
     2461 actttgatga ataaaatcac tggaatttct atctttgctt ttccattact ctatatttgc
     2521 tttggtcttg atgttgcagg aattatgata tgtatcgtgg cttttctttc ttcttttgaa
     2581 gaaatagcaa ttatgataaa atcaaaagaa ttaaatcgtg atataaaaag tatatttatg
     2641 tgacaactta gaatttgtga caaaaataat ttatataata atgaggaagt aaaacataaa
     2701 aaattatcca caagtttatg tgtaaaatag ttttaaagca atagagttgt attgtaaagc
     2761 ctttagtgca taaattacat tttaaattaa aaatgatact gaaactgctt atcagcactg
     2821 cgaattatcc gcaaatagtg aagacttttt agatttaagc taaaagttgg gaaaaaattg
     2881 agttttaaaa cttacactct tcttatatag aaaaggagag tgttttttat gattataaaa
     2941 gcaaaacaat tatcaaaaat atatggttct aataacaata aagttatagc acttaataat
     3001 gtaaatttag aaataaactc aggtgaattt gtttctgtaa ttggaccttc tggaagtgga
     3061 aaaagcacac ttttgcatat tttaagtgga ttagataatc ctacctctgg acaagtgtta
     3121 cttgatgata aagatatata taaacacact gaaaaagaac tatctgcact tagaagaaaa
     3181 tcctttggat tcgtctttca acaatttaat cttttacctg ttcttacagc atctgaaaat
     3241 atttcaatgc ctgtacttct agataaaaaa caacctgata aagactattt aaatgaaatt
     3301 tcatcattac ttggaattgc agatagactc aatcatcttc cccatgaact ttcaggcggt
     3361 cagcaacaaa gagttgcaat tgctagagca cttattgcaa aaccagatat aatatttgct
     3421 gatgagccta caggaaactt ggacagtaaa agtggcagtg aagttatgaa tcttcttata
     3481 aaaacttcaa aacagtttgg aaagacttta gtggtaatta ctcatgatga tagaattgca
     3541 aaacttgctg atagaaaaat ttcaattatt gatggtgttc ttatggaggt gaaatagaaa
     3601 tgaagaatta cctttcactt tcaataaaag aattaaaaag ccagaaattt atgactacat
     3661 tcatcattat agcaatagta ctatcaacta ttatgacaac tgtggttgga cagtcaattg
     3721 gtatcctaca aaatttaaga attgaacaag cccgttcttt taatggtgat agacatgtat
     3781 cctttcacca actcactaaa aatcaagttg atgacataaa gaaagatgat agagtttatc
     3841 aagctggtac ttcaataact attggcagta gtaaaataaa ggattctggt atctctgtac
     3901 ttgtaaaaga gtatgataaa acaggacttt ctaactaccc aaaattaatg aaattaaaat
     3961 ctggacattt gcctaaagat aaaaacgaaa tagccttaga tgaaaacaca ctaaagctta
     4021 tgggtataaa accaagacta ggagtgacta tacctatgaa tttagatatt tcacttttaa
     4081 atgataccat accaccatat aattacacag ctaactttaa attgagtggc attttagagg
     4141 atgattatac aggatatgta agtggtatag taaatggtat tgtaggtaaa ggtacatcag
     4201 aaaatctatt acctaagaga tatattttat cctctttgga ttttaaaata aaacagcaag
     4261 aaaagtttca agaaatagta aatcaattag ctaaaaaaat aaatctttct cacaattcaa
     4321 ttcaatataa ttggatttac ttaaatgctc ttaagataca gtttgaaaaa gatgaaaata
     4381 gttctaatag tgatggtata tctatgatta tattggtatc tttatttgtt gccttattgg
     4441 ttttactggc ttctggactt gtaatttata atattttaaa aatctctgtt acaaagaaaa
     4501 ttaaagagta tggatgttta agagcaatag gagctgaacc aaaccagata tataagatag
     4561 ttattttaca aatcttaatt ttatgtactg ttgccatacc tattggagct gtaattggaa
     4621 ttatatcttc taaaggcata actggaatgg tcactaatat cttaaatcca gatatacttc
     4681 tagcaaatga taataaagaa attactgagc ttatacataa aaatacaaca gcatatatat
     4741 tcccattagt attaagcact aatgtatctt tgattttttc atttatttca gcattaccat
     4801 cagcaatata tgcttcacat gtctctccca aaattgctat ggttggtagt actaccaaaa
     4861 tcaaaagaaa aattaaaaga gaaaaaacaa taaaaaactt tgaaagacat ctagcatggc
     4921 taaacttaaa aagaaataaa ggaagaacta taataactat actatcatta tttatgagta
     4981 taacagtatt cgttgcactg agtgagttct ctaatgtatt agatgtatct aggtcagtaa
     5041 gtaatttaaa agaaggagat ttttccttaa caaatgaaat atctggtttt gataaatcaa
     5101 cactagataa aatagctaaa atgaaaaacg tcaatcgcac ttcctttata aagtactctg
     5161 aatacaaaca gggtgaaata gatacagata taaattttga aaatagtggt gagatgttaa
     5221 aaatcattgg aattgatgag caaactttaa aagacttaat gccttctatt acagattcta
     5281 tactagaaga ctttaaaaat ggttctatct gttttataaa aaatccgatt gctatttcta
     5341 caccaggtgt aaaaactaga tatacaaatt taaaacctaa agatagtatc acaatcaaca
     5401 aaaaacaact agatatatat agtacagtag ataaaatgtt ctttttacaa ggcaatggct
     5461 ggataaatgg tgttgatgtt attgtatatg attctgttta taacactttg acaaataaaa
     5521 acaagttcaa ccagataaat atttatgcta aagataaatc aaaattagaa caaataagat
     5581 tgtctattga acagatttgt gaaaataatc ctggctctca ttggatatca tatattgaat
     5641 cagataaaca acttaaagag agttttaaac aaatagaatt tctagcatgg gcagttatat
     5701 tatttgtggg attgattggt actttaaaca tcattaacac aactcataca aatataaata
     5761 ctaggactaa tgagattgga gttaaaagag ctattggtat gagtaatagg agcttatata
     5821 aaatgttttt atgggaaggt gtttattatg gaatttttgc tgctatattt ggttcaattg
     5881 ctgggtatgc cagtgctatt atcataaaca tggcaactat agaaaagctt gatttcacta
     5941 atataccaat tacttcaatt ttacaagcta ctattatttc tgtacttgca tgtattatag
     6001 ctactcttat tccgttaaga aaagttaaaa agatgaatat aattgactgt atagatatca
     6061 atttataact attttacaga taataatata aattaaatta tcaatgactg tctctatttt
     6121 ctttcaaatc taactaataa atctttttaa ctataatata agctttaaaa aaggcctgtc
     6181 tcaaaataga gataagcctt ttttcattgt cgaaataatg aaaatatatt taacttcaca
     6241 aaaaaagagt gcctcacgaa ttaaaaattt cgctttgaga cactctcctt taatttaaaa
     6301 tatatagtta aatgctatgc caaaatttca cacatgctcc acctgatttt gaattttcta
     6361 ttttaacaga acctccaagc tgttccacta attgtttgac aatgtaaagc ccaagaccag
     6421 aatgtccacc ttttgtctgc ctagcttcat ctcctcgata aaatttatca agagcctttt
     6481 ttaaatcttt agagctaaac cctctaccag aatcacaaat ctcataagaa atacaatttt
     6541 tttcatcctt tacagtaata ctaatattac cacctgatgg agtgtattgc agactgttgg
     6601 atatgatatt atcaaaaatt cgtgtcaacc tatcaacgtc gatttgaatt ggggattgta
     6661 tattcccctg cattttcaat ataagttcaa cttccttttg atgagcctgt aattcatagt
     6721 cttgcacctt ttgcctcaaa aattcaggta aatttatagg aacaagattt aattgtatat
     6781 ttgacttttc taagtcagac gtatattgca tcttttggac aagagcagca cttttttctg
     6841 tattttctct tatcactgta agatacctat gaagtttttc attgtcatct gtattattac
     6901 caataagtgc atctgtatat cccaaaataa tagaaagagg agattttaaa tcatgtgcta
     6961 gagcttccac catttcaaca cgttcctgct ccatcttcca ctgtgcagat aaagagcctt
     7021 ttaattcgtc tttcatttca gaaaaggcac tacaaagttt accgagttca ttatctgaat
     7081 aatagtcgat ttcaaaatca aggtcctttt cttttatctt cttggacgca tcaatcaata
     7141 gatgtaatgg ttgattgata ttctttacaa agcgcttaga aagccatcta gtaaaaccca
     7201 cgatatatag aaatggagaa aacaaagcca ttataataac tgcaaataca aatctccctc
     7261 taatatttgc aaaggtgatt ttcacttggt agaatagaat aacagcaccc tcaatctttc
     7321 cattatcccc tttgattgga acagtatgga tataaccacc tttacgaaca gttttattga
     7381 taaaattatt aaataattct tcttttgttt taaatagttt tttggggtta gtaccatata
     7441 aaatattgcc attattatcc acaacttgat atagcatatc atccccacga atcgttcttt
     7501 ttaaaccttc ttcattcgat tgtgataaca aagctatgtt tttttcattg atgtattttt
     7561 caattctagg aacttgtcgc tcataatagt ttggtggata gatatcctta tttaaggaat
     7621 gaatatacaa atagagagca aatacataag taatcactgt ggcaactata ctggctatta
     7681 taataaaaat aaaagtatgt ctaaattggg taataaggga ttgatttttc atttaataac
     7741 ctctctctct ttacatttta ttccacttat acccaatacc ccaaactgtg gaaatatatt
     7801 ctttttctgg tgtcacagca gaaaacttag ctcgtatttt ttttactcgt tcaacaacag
     7861 ttgaggaatc accttcagaa tcacaccccc atactttttc atagatttgt tctcttgaga
     7921 aaacttgtcc tgcatggagt gctagtaatt caacaatatc atactctatt tttgttaaat
     7981 caatattctt accatcaatt ttaattgcac gttccttcat atataagcat aacttcccaa
     8041 aatacaaagt agatctcttg ttctcttcat tgatatactg tgaacgcttt tccctacgca
     8101 gattagcttc aatccttgcc ataagttcac gcagtccaaa tggctttgtt acataatcat
     8161 cccctccaaa ggtcaaccct ttgattttgt cggtttctga ttgctttgca cttaaaaata
     8221 taattgggca taaaacatca tctctaattg cctgacatac ctcaaatcca ttcattttag
     8281 gcatcataat atccagtata ataaggtcag gctcctgttt tgataactct atacctgcct
     8341 gaccatcata agctatcaac acttcatgtc cttttgcctt caattcatcc tcaagtagca
     8401 ttactaggtc catttcatca tcaataatca atattttact caatataaca ccacctttct
     8461 cttatgggaa ggtcagtatt aaaacaatat tcgtctactc ataacttttc cttccctccc
     8521 acattttaaa ccaaataacg cctcctatca agaatataat caaacctatt gctactacta
     8581 taagctctat actagctgtt tgcatcactt tagagtaaaa tgcctcagtt gaaatagttc
     8641 cagcttcttt aataaaataa attcctggaa gcatacctag ttttactggc catgtccatg
     8701 gaacaaatgc ccatactttt tcaccaacgc tagttaatcc aagtattgct gccatcaaaa
     8761 ttccaccaat actaatacca atagatgcac ccatacccca tgcaaaactc acccaaagat
     8821 gaatagctaa tagtggaagt gtcccaatca ccacaagtcc tgctgctgac aggaataacc
     8881 atattgttgc tccagatggc actgcaatac tcattcctat gcaaagtatc aacgtagcaa
     8941 taaatgtgca tatagttagg caaaacagaa gaaaaagaaa ttttcccaga tataatctga
     9001 cacgagaaat acctacacat aaaaaaccat taaaatttcc agcaagctct tcctcctgca
     9061 ctataaatcc tgctaaaata cctactccaa taggaatgat gaatacagtc catactgtaa
     9121 aaaatccttc aaaagcaaat tcttgtgtac ttccagattt agttgtgaga taaaaaacca
     9181 cacataatga aaaaataata ggcatacaaa gagtaagcca ctggacagct gtcctctttg
     9241 ctcgaagcca ttctgatgat aatagtttta tcatttgtca tccctcctat tgaaccaagc
     9301 tgcagtaagt gctgttaata ctataaacac aaccaaagaa acaacaatcc caataggaat
     9361 cactgaagta ttcaaaagtg ggtctccagc ttctaatata gtaccgttag gatgtatgtt
     9421 gatgattggg cataccattc taactgccca actccaaggt actgcaatcc aaaatggttt
     9481 tgtcgcagca agaacaccaa atatcatacc taaagctcca actcccatac ttaggaacat
     9541 acctttccat gtagctagcc atagctgaag tggaagtata gcaagagaaa caacccagca
     9601 tacaatactt gcccctataa tctgtccaaa aggaactgga ccagctttca atattaaacc
     9661 agttataatt gttacgacta ttaaaactag cgttgaaata aaactgtaga ctgccatagc
     9721 tataacttta ttaatccaaa gtgtcatagg agacacattg tgtgtacgta aagcacgata
     9781 atttccagct tttttttcct ggaaagctac caaagtagcg aacaatgcaa atccaatagg
     9841 cagaaataaa aatgaccacc agttaaaaat taatgctaaa agattttgcc acgcccaaga
     9901 agttgtttga gataatggat tttgcatgag tgttgattgc attataaatg catatgctgc
     9961 aaaagacact gggaaaaaaa caataagttt acccataaat gttcttttat actttaaaag
    10021 ttctgattgt aaagcattca tctgttttgt cctcctttaa gaataacatc tgtaaaaaat
    10081 tcttctagat tttcattagg gtcaggcatt ccttgaaata atagtcgtcc tccatttata
    10141 attcctatat tatctacaac ttgagcaacc tctgatagaa tatggctaga aagaattaca
    10201 gttatacctt ttttgggaaa agaagcaatc aattcacgca attcttgaat gccaaagggg
    10261 tcaagaccat tggttggttc atctagtatt aacaactctg ggtcactcaa aagtgcaata
    10321 gctattccta acctttgttt catacccata gaaaattgtg aagctctctt tttacctgta
    10381 tttttcaaat ttactatttc caacacttca taaattttat ctttaggtat tcccaatatt
    10441 tttgtatgta ctaacaaatt ttcctctgct gtaaggtttc catacaatgc aggggattct
    10501 attaaagaac ctatttttgc aagagattct cgtttccaag gctttctatc aaaaataata
    10561 cgaccatttg ttgggcgaag aagaccaaca agcattttaa gtgttgtgga ctttcctgct
    10621 ccatttggtc ctaaaagtcc atatatacta tttcgttcaa tttgtagaga aacattgtta
    10681 actgccattt gttctccata tctttttgat aagttttttg tttcaagaat taaatctttc
    10741 atattaattt ctcccttctt gtaatacctt aaatctgtta ctaagagaat actaacatga
    10801 aaaaataaca attctataac acctactaat tttaataaaa tttaaaataa aaaatagagt
    10861 taccaatttt attttggcaa ctctatttaa gtttatgttt tacagttata ttagatttaa
    10921 acttagtttc attatcacac taacttcaaa tttactctat aatctttaaa tcaaacacta
    10981 atttattaat caaacactaa tctattaatt tatatttctt tagacatcta atatagtttg
    11041 ctactaaata ataattattt atatcatatt tatctaatcc tttaaaagaa gttatttcat
    11101 cattaagctc atcactacct tctacaaaaa cacaacctgt ttcaaattcc atacctacaa
    11161 taatcttaca gtcactataa atttcctctt gagtatcgta caattttttt gcatttcctt
    11221 catttggaat tgtaaataca ataggtttta attctttatc actggaaatc ttttgtgaaa
    11281 aaggcttgtt taacttaata ttattgataa ctacatgttt aaattcctct gaagtttctt
    11341 ctactattac atattttcgc tttaaaaact ctattatttc taaaacagac ttagaattta
    11401 cttttaaaat acttttatat ttttcatata aagatttcca ctgttcaatt ttttcctgtg
    11461 gtatttcttg gatatgtgta cttatttttt cttgagatat ttttttcaat taaactctcc
    11521 tttcaatact taaatatcct tatgaatctc acatgactaa agttacaagg tttctactta
    11581 atagaatttt gcatacttaa atatgtctta ttaattctct acaggatatc tccgtagttc
    11641 ctacgattct tacataagtt acttatttta aattgttatt agttatattc taaaaattat
    11701 ttattttatc actaatataa tactcttaaa aactttacaa atcaatttaa atttgctacc
    11761 ttttatttat aacaacttat agtacatttc tcctatgctt ttcaatatat tacttttaaa
    11821 aggaggtttt cattatgatt ttaaatagag ctgagctttt ccgaggatat tctttaaata
    11881 gatttaacga gatatgcaat atattatcag taaataaaat taaatataag tataaagtga
    11941 aaaataatgc ttactctaaa aaaccttttt ttaatgaaat aacacttggc aatttaggcc
    12001 aaaaggaaga tttctcttat gagtacttaa tttatgttca taaagacgat tatgaacagg
    12061 cagagtctct aataaattct aagttgcgat agattatcta taaaacatga cattctgttg
    12121 tcatgtttta tatgttatta tactttcaaa tatgctacta tattttcaaa attgtgtgct
    12181 ttaaaactag ctttttttag taatttaaag cttaaaacta aggagactcg ttatatgaaa
    12241 ataaatataa aatcaaagtt aagtgaattt attaaacaga acaatctttt tgatgacagc
    12301 agaataatat cctatcttcc taatataaca atagaaacag acaaaataaa aactataatg
    12361 attaatgagg ttgtcccaac taacaccaat gacgattttt atagtgtaga taaagatgct
    12421 gattatttaa aaactactat tcctttattt gacagtgcag gaataaaagt atctaacata
    12481 aatgatatcc tagatatggg tatctatata actaatgcag taaaacttcc taaatcagaa
    12541 tatacaataa ctcgtgatac aattaaatta catatgccta ttttagaaga agaaattaaa
    12601 ttatttaaaa acctagaggt agtaatgtta atgggagatg ttgcaaaaaa atcttttaat
    12661 atgattacta aaaagcatat aaaaaagaat gtcatccctt ccatttcaac atataaactt
    12721 cgtaataatg aactttatta tgataattta agagtatttc catcatatat tatgactggt
    12781 ggaaacatac tgattgaaaa atcaaaattt gaaatggcat cagaagatat aaaaaaaatg
    12841 ttcgagatta taaacggttg atattacaaa ataataaaaa ctaatataaa ataaaaagtg
    12901 cctcaaaaac ttctatttat gaggcacttt tattttatat tagtgtaact atatactatt
    12961 aacataattt attatttata ttgttctttt taatctctat tccatttagt ctaggtaaca
    13021 ttactcttta caagattact ttatatttct tttatatctc taacgttttt atttaataaa
    13081 acaattatta atacaagtca aatatatcta tttatctaaa aatatatctt ttacagtaat
    13141 atctggatgg tgatacttct tcctattcgt taaaaaagat ttttttacat taatagcctc
    13201 ttttggacac cagtgaaaac aagctaggca tctttcacac tcattacccc actttggctt
    13261 tttatcaact atacttatat tctctacagg acaaaccctt ttacatgtcc cacatccatt
    13321 acatttattt tctgcaaaaa atttagtagt tgcaatttgc atattcttac ctactaaacg
    13381 aagtacaaca ctatctatat gtgactttaa attgtttttt ccttctatat ttccaatctc
    13441 caagttattg atacattttg caatctcagc taccctatta gtctgatttt tcaatctttc
    13501 ttcttcaaca tctggaggtg ttatcaaagc atttccaggc atattgatta aaaaaccagc
    13561 agataatttt ttcccccttt ttttaagtaa cttatctata gtaaataaac tatgtccagc
    13621 taccccatta caagtggcaa tggcatatat atgagtatta atattaaaat ttaatttctc
    13681 tacaaaagac tttactatat caggaacatc agcaaaataa acaggaaaaa caattaccaa
    13741 tatatcttca tttatgtcta tattattttc ttttctatgt atagctaagg gtataacttc
    13801 tttacttcca tttaacgttt cttttaaaat atttgaaact actagcgaat ttcctgttcc
    13861 agaaaaataa taaatcttac tattcattct tatccctctt tctatatttc tacatcatca
    13921 atagttaaaa agtctcttaa aataggattt atatttactt tccaattcta tccaaaatat
    13981 cagtaaacaa aggcaattct tcatcagaga tagatttaaa tgtctctgtt gcatatatac
    14041 ttgcaatttc atttaatggt tcttctaatt ctctacctaa atctgttagg aatatttgaa
    14101 acttacgtct atctttaaca tctactttac ggacaattaa tcccttattt ttcataacat
    14161 ctaaaattct tgttgtatta gatttatcct tacatgattt ttcacataaa tctatctgag
    14221 aaatcccatc ttgctcccat agaaacctta ataacgcata atggtcagtt gttatatcat
    14281 atccattttg tttaaacatc ttttttaaat catcttttac ttttaacgaa gtacttacta
    14341 acttagttgt agcttcatat agtttctcaa aatctttcat tttacctcca tttaaataat
    14401 cccttgctcc tcccatctat caattattcc cttatattgc tcaatatctt taaaagccat
    14461 tggctttaaa acagcattta tagcaataaa attatagatt tgagaaagca caggtggatt
    14521 tttcttttcc tctatgcaat aaataaattt tttagatacc ttttgtatct ttgattctac
    14581 tttttcagtt ataggatttt gattaaatcc agtttttata gataaactac cacaaacata
    14641 acctcccgaa tctaagacca tggattttat aaaagaaatt gcttttgaac cacgtgcatt
    14701 ttcatatgta gtaactgcta tacatggctt acgatacaat aatctttcaa gcatcatatg
    14761 tgctctatct gtaaacacct tgaaaagtcc agttacacta cttccataag ttgggctacc
    14821 aaatataaca ccatcactct tagtaattat atcatgaata tactctactt tatcattttg
    14881 attgatacac ttgcccattt tataacaatt taaacaacca atacaatatg atggatttac
    14941 ctcacttaaa tcaaaataat taatttttac atctggtatt aatctttcta tatctttata
    15001 caagtaagtt aaaacttttg atgtagctcc attttttcta gggcttccat ttataactgt
    15061 aattatcatt tctatatctc ctttttagtt gttatatcaa caataagtat atcaactata
    15121 attttagctg tcaatataaa ccatttattt aaagctatac ttacacagct taaataaaag
    15181 ttttatttaa ctacatgtgc ttatttaatc aactataaat gaattatttt taataattga
    15241 caaactaaat taatgataat atactttatg tattacaaat gtaagtagaa ggaggtatag
    15301 tatgaaaaag ttaccacata tttcagaagc agaatatcaa gttatgaaaa ttatttggaa
    15361 atatgctcct atcagcacta ctgaagtaat tgaaaagttg gtagaaacaa gtacatggag
    15421 tcctaaaaca attcaaacaa tgttattgag attagttaaa aaaggagctt taacatatga
    15481 aaaaaatagc agagtattcg tttatactcc acttgttaaa gaagaagaat acgtagctac
    15541 agaaagtagt tcttttttaa atcgttttta taatggtact cttaattcta tggtactaaa
    15601 ctttttagaa aatgataaac tatctgaaga tgatattgag gaactgagag aaatcttaaa
    15661 taaaaggacg actaaggggg ataaataaat atgtatgccc cattctttat acgattttta
    15721 ctcagtacat taaccttatc aatattaatt gctgtaattt tacttgccaa aaaagtattt
    15781 aaaaaacata tttctgcaaa gtaccaatac aatattggat ttatattgct tataatgtta
    15841 ataattccat ttgtccctct taaatatatt aatcttggaa atgtatttaa ctatttatat
    15901 acttttaatc aacttggaag cagtactaat aggacttcta tgataaaaaa tagtccaaat
    15961 tctataatta ataactcaaa tgcattaaac gatttttcag tatccctaaa ccaagttaat
    16021 tttgatagtt taaatatttt attactaata tcatggataa ttggaatttt aatcatgata
    16081 gctattacta ttcattgcaa cattaaaata aaaaatatga aaaaatcaat acaaatttta
    16141 agagatgagg agatagtatt tatatttgaa aagtgtaaaa aagatattgg tataaataag
    16201 gattttatac tatgcaaatc acctattata aatacaccta ttacatttgg atactttaaa
    16261 ccacacatta tattaccaga taagtctata tctaaattat ctttaaaaga tataaaatat
    16321 atcttacttc atgaattaca acactttaaa aataaagata tactgataaa ttatataatg
    16381 tgcttccttc aaatattata ttggtttaat cctcttgtat ggtatgcatt taaagaaatg
    16441 cgtatagacc gtgaaattgc atgtgatatt tcagttttaa aaaagcttga taaagatagt
    16501 catatagaat atggacaaac tctaataaac tttgtggata atatttcaag accatcaaat
    16561 cttacactta catcagatat tggtgggtca aaaaaacaaa taaaaaagcg actattgaag
    16621 ataatggatt ttcataatga atcaaatacg ttaaaattca aaagcatttt aatttttaca
    16681 ttgatagctt taattgtatt tggaaatatt cccaaactat ctattatggc agatatcaat
    16741 gaaaaatata gacctacaaa tgaacaaact gtgtacgagg acttaagtca gtattttaaa
    16801 ggttttgatg gaagttttgt tatgtatgat ttaaaatcca agcaatatca tatttacaat
    16861 aaagaaaaaa gtaaaacaag aatttctcca aattctactt ataagattta tagtgcctta
    16921 ttgggattgg aaaatgggat aataacaaga aataattcac atatcccttg ggatggaaag
    16981 catcattcta atagttcttg gaatgaagac caagacttat tttctgctat ggaaaactcc
    17041 gtaaactggt actttcagac tctagataaa aaaacagggc taaaaaacat acaaacttat
    17101 cttaggcaaa ttggatatgg caattatgat gtttcaggtg gtatctcaaa cttttggcta
    17161 gaatcttcat taaaaatatc acctattgaa caggtagaat tacttgaaag tttttataca
    17221 aataagttta aatttgaaga taaaaatata gaaacagtaa aagattcatt gttaatatca
    17281 aaaaaggatg gggtatcact ttttggtaaa acaggcactg ccaatataaa tggaaaagat
    17341 gtaaatggtt ggtttatagg ttatgtggaa aaaaatgaaa atacttattt ctttgctaca
    17401 aatattcaaa gcaaagatta ttctaatggt agttcagcat ctaagataac attatccata
    17461 ttaaatgaca aaaaaatcta taatccttaa tatatttact gtacattcta aaaaattcac
    17521 ataattttca tattaattat caattaaatt catatacaag gcttattata taaacataga
    17581 agatagtaaa actagcttaa aaacataata taatacccta tacaaatagt ggcaactctc
    17641 cctcacatcc tcaattgttg ccactattta attttttgta tgaagttaaa tatattttca
    17701 tcatttcaac aatttgggtg caatacaatc tctattttga aacaacctct ttaattgttt
    17761 tagagtttat gaattatttc tcatacttta aaaagattga attactcttg ataatcttct
    17821 tttgtagtca ttacatatca cctactactc aacacatatt ctttgaccta tttttacatc
    17881 cttgtttata tccaaaatat ttaccttctt accatctgga aacaatacca ttgttaaagt
    17941 agactttcct aattcatgta ttctctttaa atcaacatta acaagaacat ctccctgttt
    18001 aaccatatct ccttgtttaa cagcaatatc aaagccatct ccatttaatg caactgtatc
    18061 aattccaaca tgaattaata cttctccatt agttccttta atcccaaatg catgtccact
    18121 tgaaaccact gtttctaatt tacctgaaat aggagaaaca atttttccat ctataatatc
    18181 aatagcaaaa ccatcaccta acattttttt agaaaatgtt tcatctggta cattttctaa
    18241 tgcaacaatt ttccccttag ctggagacac aaagttttca ttattttcat taaacactaa
    18301 ttcttcgtct tcatcttcat ttatagcttc tttaggcact ccaaataaac atgttacaat
    18361 aaagcagaat gcaaccacaa atattgatgc taataagtat gtgattaaat tatatccatc
    18421 atagatatac attaaataag aaggtaaaac tgctaatcca tatgaattag attgaatatg
    18481 taaaattgat aatattgctg ctcctaaacc tgaagatgca cacataataa tcaaaggctt
    18541 taaattatat cgaagctgaa tcccaaataa aacaggctca cttataccaa aaagttgaga
    18601 tagcattgct ccaactgcag tttgtttaac ttgattattt ttagattttt tcataaatgc
    18661 tagacatgtt ccaatattcg caaatccata cattgcacaa agagtaataa gggcattaaa
    18721 tcctgtattt gctaatagtg atgtttcaat cattacataa gtatgatgta agccagtaat
    18781 aacagctaat ggatatgtaa atccaataat aaatcctcca attcctaaag gcaaatcaat
    18841 caataagctg atgatatcaa ccatttttaa ttctattgta tgcattatag ggcctacacc
    18901 taacaaaata actaacattg ttattagcat aacaaaaaat ggtgtgaaaa taaggtctaa
    18961 tacattaggc atatgtttac ggaaccattt ttcaagatta gctccaataa atgctgtaac
    19021 aatagctgtt aaaacacttc cttgacaacc tacaatagga ataaatccaa atgccataat
    19081 tgcttcaatt tcatttgatg ggtcagcaac agaatatgca ttaggaagta ttggggaaac
    19141 taacataagt ccaataacaa gtccaataac aggtgttcct ccaaatactt taaatgctga
    19201 ccatacaata attgctggta aaaaagcaaa tgctgtttct gtaagaaccg aaacaagtgt
    19261 ctgaatatac aatggaatat ttgcagaaga cattccaaat aatccaagta cattatcatt
    19321 aaacaaacaa ccctttaatc ccaagaataa tcctgttgct gcaataactg gaataatagg
    19381 aacaaatata tctgctagag ttctcataac tttcttaaat ccagtttcat tatttttaac
    19441 taattcatct tgttcttttt ttgataaagt ttttaatccc attttttcaa cttctgcata
    19501 aactttatta actattcctg ttcctaaaat aatttgatac tgtccagaag taaagaaaac
    19561 ccctttaacc tcatcaactt tttcaacttt cttatcatta ataatttctc tatctttaac
    19621 catcactctt aatcttgtgg cacaatgtgt aatagaaata atattatctt caccaataat
    19681 tttaataagc tcatttgcta ttttattata tttattcatt tttctacctc actttcaata
    19741 atactgcatg aaatggtttc aaacgcactg ttttatttaa tagatttata tcatctaaat
    19801 tttggaatac aatatcatat ttttcttcta ctcttatttc tacaggttta tcagaaaaat
    19861 tataataaca atcaataact accaaatcta attgccttct atacgcaata atatcatcac
    19921 tttctatatc aataggttct attgaaccat aaatcaaaca atcagaatac tcactatctt
    19981 gtctcaactt tatcatcttt ttataaaatt ctaaaatact ctctgaattg ttaatctgac
    20041 tctgtgcatt aatatcttta taattttcaa taacctttaa ccatggtgtt gtatctgaaa
    20101 aacctgcatt ttctttatct gcccatggaa atggcgttct tgcattatct cggcttctta
    20161 aattcacaaa ttctaacgct tcttcttctg agtaaccttc atctattgaa cgatagtatt
    20221 ggtcaatgct agataaatca ttaaaatcat ttatggtatc tcttttaaaa tttgtcattc
    20281 ctaattcctg tccctgataa ataaatgggg ttcctcttaa aaagaaatac attgctccca
    20341 acatttttac tgcatcatca ttcctttcgt attgcaaaag atactttgtt gtagctcttg
    20401 gttggtcatg attttcaata aaattagctg cccatccatg ttcctgaaca tacatttgac
    20461 tatctaaaat gcattttctt aaatctggaa ttgtccatga aattctttta aaccactcac
    20521 taccagaagc aacatctaag tcagcatgtt taaagtcaaa gaccattgaa aagaaaccat
    20581 cttctccaat aaaatcacct aactggtcat agctaatacc tgctgcttca gcaacagtca
    20641 tacaatcata ttttttaaac gtattttctt ttaattcata aagcattttt tctattccag
    20701 gctgatttct aactgttttt gtacatttaa caagaccatc aacaccatca ctttctaaac
    20761 tttgaaaact taaattcttt ttgatgaatg taatagcatc aactctaaat cctgccactc
    20821 ccatttgaga ccaggtatta atcatttggt aaagttcact tcttaatatt ggattttccc
    20881 aattaaggtc aggttgtttt tttccaaaag aatgaaaata atattcgtct ctattctcta
    20941 ccttttccca aacacttcca ccaaaaacag acctccagtt atttggcaaa tcgtttcctt
    21001 ttttaaaaat ataataatca tgataaggac tttctggatt ttttaatgct tcttgaaacc
    21061 aaggatgttc atcacttgta tgattaagaa ctaaatctaa tataattttg attcctctat
    21121 ctttactttt tttaattaac tcttttaaat cttccatact tccaaattca cttgcaatat
    21181 ccaaataatc actaatatca tacccattat catccatagg tgatttaaaa attggacata
    21241 accacaatga tgtaatacct aaatctttta aataatctaa acgagaaata attccctgta
    21301 aatctccaat tccatcatta ttactatctt gaaagcttct tggataaatt tgataaacga
    21361 tttctttttg ccaccatttc ttttccataa attactcctt tttatatgat atttcttctt
    21421 tttaattctt tataaattcc attatcgaat atgtcctcac atatagatgt cgctatatct
    21481 ttaagttgtt gatgactatt tttcattgca attgtaacgt ctgatgcttg aaacatgaca
    21541 atgtcatttt gaccatcacc aaaacatatg gtttcttttt gagttactcc taatctttct
    21601 tgtaatctct taattgcttt gcccttatga aaatcttttt gaataatttc ataatactga
    21661 ctagaaatat ctctctgtgc taattccatt ttatcttgta gtatatcttt cacttcgtca
    21721 aatacctttt catttgacca taaacaaatt ttatgtatat cttgtgactt atactcttca
    21781 atgttatttt catatgttat tttttcttgg atatgttgtt tatttataca agaattagtt
    21841 cctttcaact gattcattgt ttcaaatatt tctttggctt tttgattcat gaaaactttt
    21901 tcctgactct caatcgaaaa agcaacttct ctatttttta ataaacaaac aacttccttt
    21961 atcaatcttt gattaaaaga ttgattataa agtaattcac catgatattg aatgtaatta
    22021 cctcctcctg caatatatcc atcaactcct aatgataaaa catcatcttg tattgtaccc
    22081 atacttcttc cagtacaaat aacaacactg caatgattct tttgacataa acgtatagca
    22141 tgctttgcag attctgggat tccatatacc tcatctctaa gcgtaccatc tatatcaagt
    22201 attaataatt tgtacaattt catcttcctt tcttactatc aaaatcataa tagcattcaa
    22261 aaaaaaagat atcaatattt tgatatcttt taaaaaatat gttcattttt agcacaaatt
    22321 ttcattttcg gggaatatgt tcactttcta aatacatctt gtatttaata taaagcagtt
    22381 caattaaaat aaaataaggt gttgttacat cgtagtccag ttgtttatct acagaaatag
    22441 atatagattg aatatacagg ttttcatcac ataatcttgc taaaggatta cttatcattt
    22501 ttgtaataga aaccatataa attcctttca ttctcatttc tttagcaaaa tcaataattg
    22561 tagatgcatc tccattcaat gaaataaaga aaatcatatc atctgttttt ataaaatgat
    22621 taagtgcctg aatcatatca taaccataaa catcatatat aattttccct gttggtaaga
    22681 aaatcctttt catctcttta gcaactcttt cctgagaata accttctcca aagacgatta
    22741 tacgatttgc ctttttcatt ctttcaaaca taatcgaaca atccttcttc tctatatcat
    22801 ttatcacctt attatagcaa tccattaatt ggtgtttact ttgattttta tattctactt
    22861 ctgtttcatt taagcgtata ataactctta attcactata tccaggtaat tgtagttttt
    22921 gagcaaaacg agaaagtgca cttgtagata tatggtattt gtctgcaaat tcatctatag
    22981 aaagtttaat acaatcttct ttatgattaa gaatacactc acagatatac ttatcatttg
    23041 ctgtaaatga attataatat ttattgaaaa gtccttcaat tttcattctt ttctccttct
    23101 atactctatg taattcatat gaagcaaatc taatagagca taaaatgctc ctacgacttc
    23161 ataagatata gcttgtaatg tatcaagttt ttgagtttca acatacagat tatactcaca
    23221 aagtgttgca attgtattat tatcaagcct agttaaagaa atcgtatgta tatgattgtt
    23281 gataaatttt gcaatttcaa ttcctgcttt tgtttctcca gacaaagaaa tgataataaa
    23341 taaatcactt ttttgaaatg attctctcat aaattcaatt tcacctatat caaataagtc
    23401 aataacacat ttacctactg aaagaaatat tctctttatt tcttgagcta aacttttctg
    23461 ttcattacct gtcccataaa tataaattgt ttctgcatca tatattattt tacaaatatc
    23521 ttgaaatgat attttttgta attcattcat taatagtcga taattctcgc aaacatcatg
    23581 aaaattatta tcattattaa caaattctag ttgattattt ttaagtaaat actttaaatc
    23641 agagaatgaa tctaatccaa tttttctaca aagccttaat aaagtagctc tagaaacatt
    23701 acattcattt gcaatttttt cacacgaaaa attacggtat tctctttgac aaatcaatat
    23761 ttgtttaata ataattttat cattttcatt taaattatca taattttggt taactaacga
    23821 ttctaatctc attttttcca ctccctaaat attattttaa tatacattag ttgctttttc
    23881 tagggaagag ttttaaataa taaggataaa ataatcattt gattttattt ttattaaaat
    23941 aaaaataacc tgctaaaagc aggcaccaaa aagtacttat cctttcgaac atgtacttta
    24001 gatattgctt tcttcggaag cttcatctct tcatactcta aaatagagtt ttttctaact
    24061 caaaagtatt ttccatggta gaaattactt taattcatct gccatttatt ttttatctta
    24121 tttaataatt actactactt gaatatttac ttctttaagt aaatttcata gttaacaaag
    24181 ctaaaattat tattatagat gttaaaatac aaaattgtaa gtattgttat aaatatgctt
    24241 acagctacaa atatacatga tatattaaaa agaagctgta aattattatc atacatacaa
    24301 gcaactacgc ctctattaat ccatgttaat ttttattatt taatacaagg agttccatga
    24361 gacatacata tccattcata ttttaaatta tccaattctc ttatagattt catcatcttt
    24421 ttataatccc aattccatgg attaggataa ggaactatat ttccattctt atttttgact
    24481 aaatcacctg caaataatac atcttcaaaa atcatgcaga catgccctgg agtatgacca
    24541 ggagtatgga taatttttat attcccaatc tgcatattct ctccataagg ttcaacatcc
    24601 ttaatgagcc ttctggaaat tgcctttcca ataaacttct taaacccagg tctatccttc
    24661 tctcctataa taaaaggtat atcctctaca tgagcccata cttttgctcc tgttttttct
    24721 ttcaataatt taatattaga tatatggtca acatcatgat gagttaataa aatatgctta
    24781 atatctgtaa gtttaatgcc aagtgttttt aattctttaa ttatattttt ccccataaaa
    24841 ggaagacctg tgtctataag aataatttct ttatcaaaca caataaaaca atttgagcca
    24901 cgagtactat ctaacattaa tacattttct tttatcttca taaataaatt ccccttattt
    24961 ggtattttac ctatataatt aattaataaa gttataaata gttataatac ttgatattaa
    25021 ttttattttt tattaaagta tactataggt atacaaatat tatatttaca taaatatacc
    25081 atcagtatac ttttttatca atagaaagga ataatttata tgtcagaatt atcaaaaaga
    25141 gataaagaaa aaatacaaag agaaaatgag attattgata aggcagaaaa attattttgt
    25201 cttaatggat ttgataatac aactatgaat gaattagcaa aagaagttga atatactaaa
    25261 cgcaccatat acaagtactt ttcatgtaaa gaagatttat tctttgctgt tgtcttaaga
    25321 ggatataaaa gactttggga taatgttaaa atagaaagtg caaaaggcaa aacagggttt
    25381 gaaaaaataa aactttcata ctttgctttt cataaatttt actctgtaga accttctcta
    25441 ttatgcctta tgggaatgat tggtattgta aactctgaaa aatcggatac tgatatgctg
    25501 tttaaagaaa agtttttttc ttttaataaa tttatgtttg atgaaataca aggaatgttt
    25561 gaaattggaa aaaatgatag aagtattagg catgatattg aaatacctac tcttatgtat
    25621 tcatcaatat ttacacttac aggatttttt aatttactat ctgtaacagg aaaatcatac
    25681 ctgaacaact ttaatataga tgaggaaaaa tttattgaaa caactcttgc tcttttaata
    25741 gattcaatta aagcataaac ctaagtttat aaattaaagt ttatatactt aaatgataat
    25801 tttttctatc tctaatatga aaaatattaa acataaaact aataacttag ttaatggaat
    25861 aactaaatta ctgaaaggat taaaagtaaa tggctaaaat acttctttta gaagataatg
    25921 atacaattac atttggaata aaaacttccc ttgaaaagaa aaatcacact gtatgttgtt
    25981 ataatacgat tgctgaagct aaaaaaaatt ttgataaaac tattgattta attctgcttg
    26041 atttaaatct acctgatggt attggatacg agttttgcca atatgttaaa agtatagaag
    26101 atactccagt gatatttttg acagtccgtg atgatgaaaa agacattata cgtggattag
    26161 atatgggtgc agatgactat gtggttaaac cgtttttgct ctcagtactt caatccagaa
    26221 ttaatgcagt tcttagaaga actcattcaa atactaacaa tactattaca tgtgaaaata
    26281 taagcttgaa taaggttgaa gtcaaagcat atttagataa tgttgaagta cctttgacag
    26341 caggagaata taaacttctt ctaagtctta tggaaaataa aaatagagct atgactcgta
    26401 caaagttact agaaatgctc tgggatgtag atggtaattt cgtaaatgat aacactctta
    26461 ctgttacaat aaaacgtctt agagaaaaat taaaaaatcc accttgtata aaaacattaa
    26521 gaggcatagg atatagaatg gaggacaact agtgaaaact ccaagaaata tcaacagtac
    26581 agttgttttt ataatattct gcttaattgt aactttttta ttgagtgtaa ttctaacaac
    26641 tataacttct tttaatgaat acaatgtact gtctaaggct cttggtgcat catatgagta
    26701 caatcctaag tctacagaaa gtatactaaa atcactgaaa aattctagtg ataaggattt
    26761 taaaacagga aatgaaattt tatcaaaaca tggatattct aaaagtacat tttggagtaa
    26821 aaactttttt tattttctag tgatatcgtt tggtataatt tcaacaatgg ctacagttgt
    26881 atatataatc aaacatagaa caaataagca aaaactgagt agaataaaca atcttactca
    26941 atacttagaa gaagtgaatt taggaaaaga gtctgtactt ttacgatgtg aagatgaatt
    27001 ttctcacctt gaagatgaga tatataaaac agttggggaa cttcgttgtt caaaagaatt
    27061 tgctttaaaa gaaagacaaa ctctttcaga taaccttgca gatattgctc atcaacttaa
    27121 gactccaatt acctctatgt cacttatggc acaattactt tcagaaaatt gtccagatga
    27181 agaaatagat tatattaaca gacttacaaa tcaaatctca agacttgaga gactagtttc
    27241 atctttactt acattatcaa aattggatgc aagtacttta atatttgaaa gcaaacttat
    27301 agatgtacat tctcttctta cttatgcaat tgagcctata gaaggttctt taagagaaaa
    27361 acatcagaca tttactatcg aggcttctcc ttcggtagaa tttgaaatcg atattaattg
    27421 gactttagaa gctcttttaa atattcttaa aaattgtagt gagcatatag aagatggtgg
    27481 atatatagtt gcttattata gtcaaaatcc tctatatgtt gagattatca tagaagataa
    27541 tggtaagggg tttgctcagg aagaacttcc atatattttt aatagatttt atcgtggtaa
    27601 aaatgctagt aaggacagta ttggtatagg gcttgctcta tcaaagtcta ttatagaacg
    27661 tcaaaatggt actctacatg cagaaaattg ccataatggt ggtgcaagat tcataattaa
    27721 attttacaca taaaatgctg gattataggt aaaataccta taatccagct atatctatat
    27781 tctatgttat ctattgtttt agtaatatac ttctatttac atttaaggtt ttattaaaca
    27841 aacaacttaa ctatccctat acttgtaagt agtcttaaat cgaaaccttt aaataagttc
    27901 aaatagattt tgtttatatt gtttcatttt ttatagattc tactatattt tcattcttca
    27961 actgcttgta tcctgtcata tatgatgcta ttacaataaa aagtatcaat atagtaaaac
    28021 ctattattgt agatataggt gcaaatggta aatactctat aaaatatatt tcatttatac
    28081 gtagaaaagc atacactatt cctatttgca caggaatact aagtagtaat ggtgtaattc
    28141 ctaagaataa accttccaaa ataagcatct tttttatgcc ttctggagat aatccaacag
    28201 aacgcaacat agcaaactct tgtcgtctac ttctaatatt tcctgacact gtagcaagta
    28261 catttgataa tccaatcata gccaataaac ctgtaataaa gcctactatt aaattcatgg
    28321 tctttattcc actagccttt tgtttttcac tttctaaaac atcctcaata ctataatctc
    28381 ctgaaccata gtctttatta taaatggact ctaatttttc tcttactcta ggaatgtcct
    28441 cacgattatt tactttaaat actcctatca gcctatttga acgtaaccta cttacttcat
    28501 cataattttt agatatttcc tgcaccatag acattggaac tatttgcatt aatgtatact
    28561 ttgaactagt atctactatt ttaggaagtg tgcttgtaat agctcccact ttcatgttgt
    28621 aagtataatc ccctttatct tcgtcatata ctttttcatt taatttaatg ttatctccta
    28681 ttgaaagatt taaataatct atatatactt tatttctacg tgtactacga ttaatatctt
    28741 cctccttatt atatacaatt gaggttggat tttttgtatc ataaaataac tttgggtcta
    28801 cagaaatttg cctacaatat tctgtaaaac tcttatcatc aagcccaatt atatttgaaa
    28861 ttatacgaaa cttgccatca cgttctatag gagagtactt tttagatgaa gctatatttt
    28921 taaatcctcc atttttcgca agttcttttg aagcatcttt ttcagtcaac caagtggaag
    28981 cagacatttt ttgataatat agtgaacttt ttacaccttc tgtattatct aatttttcaa
    29041 tgaaagcaga ctctacaggt tcactatttt ccaaataaac tgctatatct ctttggtcct
    29101 tataattatc acttccataa acagcttttg ctccttcttg atttgcaatt atatgcaaaa
    29161 aacatgtaag taataaaaaa gataatgtta aagatattgt tgcagtacga taagattttt
    29221 tacgagcacg tagtgcgttt gatgcaagtt ctccttctat tccaaatatc tttcctaatg
    29281 aatatttatg cgactttttg attttaacac tatctccttg tcgtattgct tctataggag
    29341 atatctttga tacttttcgt gctggtataa gtgcagaaat ccagactgtt actattgtaa
    29401 gtaaaactgc tggcagaaaa gcaggaatac cataagtaaa taccacttct ggaacatcag
    29461 tatgataatt aactgaatta acataaacta taagcctatt acacaataac catcctaaaa
    29521 ataaacctat tggtaatggt ataattgtca gtaaaaagcc ttcaaacact acagaccttt
    29581 taatttgttt tggtgatgca ccaacactag ataatatccc taattgagta agtctagcat
    29641 ttgcagacag tgaaaacgcg ttgtaaatca ccattacaaa cacggctact gtaaaaattg
    29701 ctattgtaag atacataata ggagttgaaa attttgatat actactcata gatgctttct
    29761 gttctggtga aaagactaac atctttaaaa gataattact attgtattta agattgtatt
    29821 ttccatattc attagtcttc catcccaatg atttagcaag ttttggaagt tccttatacg
    29881 tgtctcttat atttttaaat cgcaagtaca cagttatact atcattaggt tttatacttg
    29941 ttttatcaag atatccaaga gctgtatatg caggtattac tgatgaagta gaaacatcta
    30001 cctttcctac tattgtcaat gtagcctctg aaattttttt aaaactttcc cctttcgatt
    30061 ttggggctat gggttcaaga ggctttccat ttaggattct atctcctatt gggagagtaa
    30121 ttttatcacc aatttttaca cttggattat tctcaaaata ttgttttgat aatgcaattt
    30181 cattagcatt atttggtcga cgaccctcta ttatagtatc cttttctggc atagaattcc
    30241 aatactctga tgtagcattt ctataaatta tataatctct tttctcatca ttaattttgc
    30301 ctacatacca ttgtcctttt accattacat catcaactga agaaaaattt tcaattaatg
    30361 gtaagtcact tccataagta tagtcaaaca attcaccatg ccagttacct tctttctcaa
    30421 ttgaaagttt tataaaatct gtccacatgg tatacaacag tccacacaaa gaagacatca
    30481 tggttgtagt aagtaatata gctaacatta tagctatact actacgttta ttgcgtcgta
    30541 caaagtctaa ggtgtattct tgaagaatat tcatttcttc accacctttt cagagacaat
    30601 cactccatct tccatagtta caatacgatt tgcctctagt gctattttct catcatgagt
    30661 aataagaagt attgtctgat taaatcgttt atttgataag tttaacaagt ctacaatttc
    30721 ttctgaattt tttctatcaa gattaccagt tggctcatct gctaataaaa tagctggtct
    30781 atagattaaa cttctagcta tagataccct ttgttgttgt ccacctgaaa gttgacttgg
    30841 aagatggttt aatctatcag ataatcccaa tatagataca atctcagaga atctatcctc
    30901 atccaccttt cttttatcaa gtaacatggg taataatata ttttttctaa catcaagggt
    30961 aggtataaga ttataaaatt gataaataag acctactttt ctacgtctaa aaactgcaag
    31021 agcttcttcc tttaaacttg atatttctgt atcttcaaca taaatctttc cagatgtagg
    31081 tctatctact cccccaagca gatgtaacaa tgtagatttg ccagaaccag atgctcctac
    31141 aatggataca aaatcacctt tttgaactga taaatttaca ttattaaggg ctgtcactct
    31201 ggtttgattt gaaccatata tttttgtgag attttcacat tttaatattt ccatatacaa
    31261 tcatctcctt tatggttaaa ttttagcaac ctaaaatgac attatagtga catgaataca
    31321 aatataaatt actattaaaa atattgttta gtttttgcac attatttaaa attataatat
    31381 aaaacaaaaa ctatgcaata tattttattt atacttttaa tatattacat agtctttaaa
    31441 aggtattaac tttaaatcta taggtattca atttaataat caatagcaag tattttatgt
    31501 actaaaggag ttttgctcta tatagactca atttagtaaa attatttaat aaatattaca
    31561 gactataggt aaataatatt tacaaataaa attaagttta ttcccttaca ctataatatt
    31621 tcttaattat attaattgca atttccttta cctttggccc tgtaatttct ttattggaat
    31681 catctgattt aatagcaaaa taataggtgt caccttcacg ctctagcata ccaacaaacc
    31741 atgcatccac acgtttattt ttttcatcaa atcctgttcc agtttttcca tatacattta
    31801 tgtttttatc atttacatct attttcataa tatctcttaa aatattaata tgctcttttt
    31861 taaagtttgt atctccctca aatatttttg ccattgtttg cacttgttct tttggagata
    31921 tttgtaatga agattctaac caaaatccat ttaaatgtcc ctttccattt tttaaatctc
    31981 cttcccattc gctaatatcg caattcccat actttaaatc atctaaaatg ttttggacag
    32041 atttagcatc aactttatct atcaattttt tgtagtacca aacacaagac tccttaaatg
    32101 cctcttctaa actaagattt ttattccaat ttttatttgg atactctgta ccatcatatc
    32161 ccataactga ttcttttgag tttattaccc ctttttctaa accaattaaa gttgaaacta
    32221 ttttaaaagt agaacatggt gaccttcttg tttcaattaa ttctttgtta tatatattat
    32281 attctttatt tttagtatta taaaaaatag ctcctccact aataccttca aaacaatcac
    32341 tatagtcaac catattaatt ttttcttttt tgtttttatt attgttttca attttggatt
    32401 tatttaattc agacttattt aattcagatt ttatataatt cttatcagtt ttttgattta
    32461 tatcgttatg ttttttccct acataataca ttaccataac tacaccaacc aataagatag
    32521 ctatccaaat aaaattcttt tttcttttca taatttatat catctcccat atttaaattt
    32581 ataacctact attaaaatag tagacattat ttgtaatata ctccaatcta ggttctactt
    32641 ataagaaaca agactaaatg aatagctgta tctcttatat tttcttcacc tagtacaatt
    32701 atgttgatta aacctattgc gaagataatt aagtaactat taataacatc ctgtcctcct
    32761 tttaatttgt gatataataa ttgattccac atttaaaact tactattgta atatttaaat
    32821 attacaatag taagttttaa atgtcaagtt agtttgggaa agttatttta attgtataat
    32881 tgttattaca tttataaaat atggcatgtt taaaattatt ttgctattgg gaggattaag
    32941 ataagcagta ttataaaagc tataaattat aaaatttaat atataattta tagcttttac
    33001 ttttacaaat attgttttta ttgatttatt aatgaatttt attgacaggt tatatttata
    33061 taagtaaaca cttacataat aagtatataa tctacatatc tataagttga gtcaattcac
    33121 cagtatatgt caacttaagt ttatgcatag ctcgtgtaca agcaatatat aacaaactgc
    33181 ggtcataatc actataatat gttttattat ttactgaagg tacaataact tcatcaaact
    33241 ctaaaccttt tgacatttta atagaagtta tggatactcc ttttgtaaag ctagaacttt
    33301 cagaagatat taagtgaaca ctatattttt ctcttaacgc atcatataca gctttagcgt
    33361 cattatctgt ctttaatata ataccaagag tcgcattatc actcttttta aattcttcaa
    33421 tttctatttt tatcttattt atttcatctt gctcgttatt acattttaat aaagctggtt
    33481 cttcaccatg tcgttcaata ggctctaatg aagatatgtc ttgaatcttt ttggcgaaat
    33541 taataatctc aaaagtagaa cggtaactct tattcaacat aactagttcc ccatcattat
    33601 atagctgttt catatcatct agtgtatgag tgtgattagg attaaccaat tgtccaaaat
    33661 ctccaagaat agtcttttta cacttaaaaa gtaagtttat tacagcatat tgaataggtg
    33721 tgtaatcctg catttcatca ataacaacat gtcttataat cttatcttct tgtatacctt
    33781 catatgcagc atgaatatat ataaatgggt atacatctga ccattcaaga gttttttttg
    33841 ttgccatcac aaacatgttt gatatattca tttgtttaaa gaaatcctta tatagtgtaa
    33901 gagtattttt tattgtcaac attccattca aacttttaag tatacttttt acctttggta
    33961 aatcttcttc cataatatta tctgattcaa atttataatg aatgtcttca gctaccttct
    34021 ctaatctctt cttaactgga tatctgttat atgcctcaaa tctactctgt atccagtcac
    34081 ttttagctgt aaaacttcca aaagtgtagt cttttggaat aaaaatctta tttggcattt
    34141 gttttatata atcatcaata agttttacaa aatcaagtgt agatttaaaa cgcactcttt
    34201 cagaccattt agcatcattt atttctaatg ggtctttctc actttcaaaa ttaataactc
    34261 tatctaattg tacctctgct atattttcaa aacttaattc acataatggc tcttctccaa
    34321 gctcaggaag gacattggaa atatagtctc caaagacttt attaggtgat aggataatta
    34381 cattatttgc tgatatttta tctttaaatc gataaagaag aaaagcaatt ctgtgtaatg
    34441 ctatagatgt tttcccagaa ccagctaccc cttgaataat cagtgtatca gctttatcat
    34501 ttctaataat ttgattttgt tccttttgta ttgttgaaat gattgacttc attttttcat
    34561 ctgaagtgtg actaagttct ttttgaagaa tatcatcttg tatattaatg gaactttcta
    34621 gtgcatattc taattttcca tttttaattt taaattgtcg ttttcttgtt atttctccat
    34681 ccacccatcc tattggagca tcatatccag cttttccgat ttcataatca taaaacatac
    34741 tggcaattgg agaacgccag tcaagtatta taagctcatc ttcataatca aatgcaaatc
    34801 taccaatata atacttagat tcatcatcca tatctttcag cctaaaatct attctagcaa
    34861 agtatggaga atctttgatt ttattaatct tatctcgaac ttttaccata aaagccccat
    34921 agttatctat ttgttttagt ccttgttcat tttgaaacat ttcatgtggg tctatttcac
    34981 cacgattttt taccatatac attttagcgt ccatataatc cttatcaagc ttgtcaacaa
    35041 gattttcagc tttcttaaaa gcattgtcta gcttgttatt tatctctatt agatgtgcaa
    35101 tctcatctgg aaaaaaagtt aataactctg catcatattc acacatatta tgtttagaat
    35161 tagatgcaat aatatcgcaa tttactactt gtttattttc agacatttaa atcctccttt
    35221 gttaattaag tacttacact gaatcattat acttaattgt gttttggagt ttttaatcat
    35281 tcttgctatt taacttaaat gttagctttt tagctttaaa gttccttttt taaaaaacta
    35341 aatctttcta tatttattac cataagtaca cttacaagca aaatacatta aaagcagaaa
    35401 tgctcttata tataaaataa ttcaaacaaa aaatatgtgg taaacttttc atttaatcta
    35461 caattaattt tgtctattat ctctttcttc tacacaagga agaataaatt tttgagtagg
    35521 atatccaaat tccacaagca attctgcttc tgcaaagccc aacttcttta tcatatctct
    35581 atgtcctgta tcagctttgt caccctctcg aaaggtagta attgaaatat ctgtatctat
    35641 caagaaatct tcaataactt tcttcaaaaa cacttgtgca atgtctgaat tacgatactg
    35701 tggatgtatt ccaaaaaaat caatacttct agtttctcta ttaaacacca tatttgcaat
    35761 agcgacatta ttaagtttta atattaaagc tctttcttct aaaatatact gttctaattg
    35821 atatatatat tcttcctctt gcaaatttgg aaatccatct atgactaatc tcactaaatt
    35881 catccataat ggtatgtcag acatacttgc aagtttaatt tctttttcca actctagtac
    35941 attctttgaa aataggttat cttttttcaa aacaaatctt agctgcaaag gataaaattc
    36001 ttgttctttc ctatatttat ttggagattt tttatacata gctttaaata tagaagtgaa
    36061 tgcctgttga ctttcatatc cagctatcaa tgcaatttca ataattggtt tctttgaaaa
    36121 taccaataat tttgctgctt ctgtcaactt tctccgcttt atgtaatcat gcattgttag
    36181 accaacagaa gttgtgaaag ttctatgcag gtgatatttt gagtaatgaa cagcattagc
    36241 aactatatct aaatccagct tttctgataa atgctcctct atatagtcaa tagcagttat
    36301 gacattaatt actttgtaag acattgcagc ttccctcctt ttatccatgc aacattatat
    36361 caattattac ttagttatat ttaatgaatc ttgctatttt ataaacaaat ttgttgttta
    36421 agttttcaat tcataaattt aatatattac tttttacttt ctgattaaag actctaatcc
    36481 tttaattttc aataactttt catttagtaa atctaaaaag ttatttatta ttttcttctt
    36541 attagattcc ttttatattc cttaattaaa tgataaatat ttatattata cgactaaaaa
    36601 cagcttttta caattattta atatgattac aaacttaccc tatcttaaag tttaatgggg
    36661 tcctgttatg aatcttttta ttttaattta agctcttcat cagtttattt tatatattga
    36721 tgtatttgat ttcatacaaa aaagagtgct tcatgaacta aaaagttcat ttttaaacac
    36781 tctcttttat gtaaataaat ttctattcta ttttgaaata ttataatttg acttataatc
    36841 tgttaatacc aaaaataata aattgtacgt ctaagaatag attctaatta gttgctcagt
    36901 agtaaaatct gtttttaact aatcctgctt tgtatgttga acatattcta taattgtacc
    36961 atctggatgt ctcaatgtca tattatatcc tgtaggaact tttttcttat ctcttactac
    37021 aactgctcca ttatccaata gataacttct ccattcatca acagaatcaa ccataaaagt
    37081 agattttgtt tctataaatg gtttaagagc atcatcagaa ccagataata gtaaaacatt
    37141 tcctatttgc gctaattcca acccaacttc tttataggaa aaacgcaaag aacatttttc
    37201 ttttaaaaga ttttcataaa aaggtatggc tttttctata tcttgaatat aaaaacgtgt
    37261 taatatatca tatattttca tattttcccc ctatatatct atataatatt acctctaaat
    37321 tataccactc tacaaattat ataacaataa attaagcata gccttttgag catcttaagt
    37381 agaccttaac aatatattgg ggttacatta gaatacataa attaaatcac cttgatatta
    37441 tttaggataa aatttatgta gtagcagtaa atgttcttct aattatcaac taacaaacga
    37501 taatctttaa ctttctatat aatgggtaac atcattttcg taagatattc actagctgga
    37561 cgattgatat catttaaata ctggtaatat atatcgccat gaagtttata tccattgcta
    37621 tttgcccatg aaaataagtc ttctaaagta gggtcttgta attcataagg tcctaaatca
    37681 attgcagtta ttattttctg tgcaggaaca atcctttgta gaatctcatt tttcccatct
    37741 atataagtgg ctacaggaaa ccctacttca acatccaatt ttgctaaatc catgttatga
    37801 aaacatacaa tcggtgctcc tgaagataaa attcctctct cctccagata tttagaaatt
    37861 ttttcaaaac tttgttttga aaactctgaa aactctacca taaagtcaat tgtttttcgt
    37921 attgccaaaa aacagtactc agattgttca gctattgtta tattggatat tctagtcatt
    37981 ttattcctcc attactttat tttcatttct aatcttttta taccaaatta tacgaaaatt
    38041 tactgtgtaa ctaattattt catatgaata taaaacataa cctccttcca tttcttatat
    38101 cgaaagtata gcaaaaataa tctcatcatt cttgtcacac agtgctctta taatcagaac
    38161 atacttaaaa gacttgccta atttttaggc aagtctaaaa gaaatctttt tctataaaaa
    38221 atttatacaa acaattaaaa gatcaaaata tagactaata taaaactata aatttactaa
    38281 tctgaacctc ccatggctaa agtcacaaga ttactgattt atagaatttc gcacactaaa
    38341 atatgtctta ctaattcccc acaggctacc cccctagttc ctacgttctt acataagtta
    38401 tctattatca aacatcaaaa tttaaacaag tttcaggttc atcttctatt cttgctattt
    38461 taaacttaca tgcaattaat actaaagata ctattggatt tataatattt aataaagcgt
    38521 atggaccata tgttagtgca gatacaccta atgttgctgt tatatacgcc ccgcatgtat
    38581 tccatggtac taatggagag gttaaagttc cactatcttc taaagctctc gacaacatct
    38641 taggatgtat acctctcttt ttatattcct tattatacat tcttcctggt ataactatag
    38701 atagatattg ttcacctgct attatattgg taattgtaca agttaccata gtacataata
    38761 ctgttccaaa tacacccttt gctaatctta gtaaagaaga tgctactact tcaagcatac
    38821 ctgttttttc taaaatacct ccaagagata aagcgcatat tgttaaggat acagtggaca
    38881 tcatattcat aatcccacct ttagataata aagcatctac ctcctgtatt tgtgttgacg
    38941 aaacatatcc attttgtgct gcatctaata tcgtattcat agattctcct tgaaaaacta
    39001 ctgcaaacaa tactcccaat acagcaccta ctataagccc tggtatagca ggaactttaa
    39061 atacaaccaa tccaattact attgctggag ctaataataa tactggtgat aaagtattaa
    39121 atgtagaatc taatgtagac ctaattaatt ctatttgttt tatatctagt gcttgacctg
    39181 aatacttcat tccaattaca ccataaatta ctaaacaaat taataatgat ggtatagtcg
    39241 aatatagcat atattttata tgtgtaaaca aatctgttcc tgccattgct ggagctaagt
    39301 tagtcgtttc agataaaggt gatagtttat ctccaaagta agctccagat attatagaac
    39361 ctacgattaa tccaattggc atacctaaac tttgaccaat gcctaaaaga gctactccta
    39421 ctgtacccat tgtgctccaa gatgaacctg tcgcaagaga tactattgca cagactaatg
    39481 ttgatgctac taaaaaaata tttggtgata aaatttttag tccccagtaa atcatacatg
    39541 gaactactcc acttactatc caagttccta taagtacgcc tattataatt atgattaaga
    39601 ttgcttgcat tgccatcttt atactattaa acatacttaa ttctaactct ttccatttaa
    39661 atccaagtct atatacagcc acaagtcctg ctactgctgc tgctcctatt agtggtacat
    39721 gaggtgatga accatacact aatactccta gacttaaaaa taatatcaaa caagtaattg
    39781 gtatcattgc atcaaacaga cttgcttttc tacattctcg ctctttcata aaatagtttc
    39841 ctcctaatta tttaaacact acactataat tttttttgaa acatatttgc tcgtactaga
    39901 tgacatgata cccccataag ttaataaaaa ttctaaattg tcacctaatt tataatcata
    39961 ttttgtgtta cttacatcta gtatcatgtg gtctgaactt gcccctaata tttttatatc
    40021 agtgtctatt ggaattaaag agtcaatatt tatatcttgt ttacctatag ctatgattgc
    40081 cctttttaag attcccttat cttcataaac aggtttattt ctaaaggcat caactcctat
    40141 ttctccaata ggaactgatg gtttttcttt acattcaact acttggcata ttaatttaaa
    40201 tgcatcttga tatgttccat ttatattttc accgtatgcc gtttctctac caagtagaat
    40261 ggactcgcct attctcaaat tagtaatacc ttctggcata ttattattta tcaacagatg
    40321 aatagaactt gaattacctc cagatactat ttctagattt attccaaatt tttcttccat
    40381 tctttttgct atacttacca gtctgcctat attttcccta gatggtataa tagctccata
    40441 acaggttaag tttgtagcta taccaattat ttcaatattt tttaatctta ctatctctct
    40501 tacattacta aaaaagtctt cttcataaaa gtatccttct cgtaaatcgc ctaagtcaaa
    40561 cataagaact atcttatgaa taatattttt actttcacat aattcattta atttctttat
    40621 tgttcctaat tctgagttaa aactaatatc gcaataattg ataacttctt ctaattcact
    40681 taacattggg attctaatca atatcttagg tagatttata tcttgtaaat tttttaagtt
    40741 ttgtattctt gaatctgcta tatggtctat tccttcacta catatttctt ctactatttc
    40801 tttttgtgca caaaatgatt tagtaacaaa agatatctta atatttcttt catgacacat
    40861 ctgcgaaatt aattttgcat tatgctttaa cttttctata tctatttcta acctaggata
    40921 cataatcttt cctcctcaaa attcaaactt tttaacagga gcttaagaga tgcttcttca
    40981 tattttttag ataaaacacc catagatgcc ataatatagt cattatctat taatatcttt
    41041 gttttttcct ttggaaatag taattcatta tttttattgt atttgagcat attatctaat
    41101 atttcaattc tactagggag cctagtaagt gctccccctg taccaactat atatcttact
    41161 tctgttaaat ctttcccttc tgcaattttg acttttccag ttgttccata gatatttctt
    41221 atttttcctg aatgtcttag catagcttta acaacagctt cttttgtcaa cctctcaaca
    41281 aataatattt cttcttttga ttttggtatt ggaggataat tctctattac tgcatcaata
    41341 tctatagata gttcactttg taagttttct ttacctataa cttctactat attcttcata
    41401 tttacatata cacctaaatc cccctcaaca cttctctttg cagtaggttc aggattaacc
    41461 aaaattttat taatatagtc acttccatct gtaacagaat gtacatcagt agttgctcca
    41521 cctacatcca gagttaataa atctccaata tttttatata aaagtttaga agcttccatt
    41581 acagctcctg gggttggagt aatattttca ttaaccattt ctttaatgta tttcattcct
    41641 ggtgctgtag ttatgtgttc ttcaaataca ctttgaatga ttcttcttgt aggttctata
    41701 ttcaataaat ctattttagg gtatacattt tcagttatat aaagcttata attagtatct
    41761 tcaaatatta accttatttc ctcttgattt tcaacattac cagcatatat aactggtatg
    41821 cctagattca tagaagctat catttctgca ttataaatag ctgtatctct ttctccataa
    41881 tcgactccac cagctattaa aattatgttt gggtttattt cttttatttt atttaaatca
    41941 gttcttttaa ttctacctga tgttatttga cgaataacag ctccagcccc taaagctgct
    42001 tctttagctg cttttactgt catatcatag actaatccat gaacagtcat ttttaatcca
    42061 ccagctgcac tagaagttgc aaacatatca ttatactcta tatcatctac agataattta
    42121 ttagctaaat ctttaatagc acctgataag ccatttctta catcgccacc ttcaaacact
    42181 gttgttggag cttgaccttg acccaaaaat ataggattgt tagtattaat atcgtggaaa
    42241 gcatttacca ctgtagtagt acttccaatt tcagctacca acacatctat ttttaaagaa
    42301 accttgctat tttccattat ttattactca tttctcttct tttttcaact aagaaagtgg
    42361 ctacatgtat gcctttactg tttcttccaa atccagcatc tatgccttgt tcaacagcta
    42421 tttcaggtgt tacttgtgtt cctccacacg caatcattac tttatctctt ataccttttt
    42481 ctacacaata atcgtgtaat tttttcatgt ttttatagtg aatatcatca tgacttatta
    42541 ttgtagatgc aagtattgca tctgcattca gttcaatcgc tgcatctacc aatttttcta
    42601 caggtacaga agtccctaga tagtggcatt ctatgccata tttttcaata cctccatgtt
    42661 ttatatctat tatttctctt aaaccaactg aatgttcatc ttctccaacc gtagctgaaa
    42721 ctattttcat aggtttttct tctatatctg ctcttatttc ttcatcagtt agtaccttag
    42781 gttcttctgg tattactaaa ttgtttaaat ctatatcaaa cggaactctt cctttcactt
    42841 ctattctagt cccttcagat tcatgcataa cttctctact tatgacctca atttcttgta
    42901 agttcatttt tttgacaaat tccaaagctg caaactctgc tgttctttta tcagtaggaa
    42961 gcatcattgt tatcattaca attccatctg cacaccattc catttcaggc ttaattttag
    43021 ttccatttct gtactcatat gattctttta gtctattttc tacattatca aaatcgtcaa
    43081 gctcatcaat atatattatt ttgtctggat tttcaaatgt acatccatct attaacattg
    43141 atggattatc tatagcattt tcatcatatt gagctacatt attgtatcca aagtgagcag
    43201 ttacaggtgc taaataatct tcttctcttt cataaacagt accttcacca actccacctt
    43261 ttatttttct agatattcca tcaccatttc tttctggata atttccagaa tcaacaaaaa
    43321 atccttgttc cacagattca aaatatccac caacttcaga tatttcttcc atatataata
    43381 cagctctttc tttaagttct cttgctttgt ctcttaattc acccacatct ttaagttcta
    43441 tcatatccat aagcccatcc ataccaacta aagcttgctt tgctgtatca catgcttcta
    43501 tattgtatat atgccaaggt acatttctac cttcatcagg tgttatagtt gattgtatgt
    43561 cagctctagt taatactgat gttaataagt ttaatacatg agtaactgtt gcttctctag
    43621 ttgatgattc catatattta gtattcattt gagctctcat cttataatca ctaaataact
    43681 ctctaagtgc aacagcgtaa ggtaagtcta tttttaaaca tggtgctgga ggtgctgttg
    43741 gtggaactgt tgataagcat atattacttt tatctatacc tattttttct gaaaatatag
    43801 agtttaaagc gtgttgaacc ataagttcag gcattacctt ccatgcttct ctagcagtag
    43861 cattagcatt atgtgctcca tcaatttgag ctatattggc aaaagccatt attttctttg
    43921 cctcacaagc atctacaaaa gaccttacca tgtttatatt cctatacaga acattatatt
    43981 gtgggtcttg atgtgctcca ttgactcctt cctcagcaaa cattactgct acttcaggac
    44041 cagcaacacc acttacatat gaatggtaat taataggtct accaacttct tcttctataa
    44101 aatcaagagc cttcctttga gctcttactt gttttcttgt tattggaact ccacctatac
    44161 cttgtggagt tccttctata agcccatcaa aatgtgattg tccagcagtt ctgataacca
    44221 ttagatggtc agctccatga tgagctgcca ttctcattct tcttatatca tcctcaaatc
    44281 taccagaagc tatttcagta gtaataactg gacttggttg agggtctata ttattgaaat
    44341 acttagatga tggtagccct acactcttct ttaaaggttt tgtacagtct ttatatttaa
    44401 aaggtcccat ttcaagattt tccaagtgtt ctctccaaac ccaacctctt cgttttggtt
    44461 tatatttatc caaatctttt aatatatagt ctatatccaa ttttttattt tcctttaaca
    44521 tgattctacc ccttctctaa ataaacatgt aacttcatcc caatattcac ccttaactaa
    44581 taacaaacct gcttctctaa ccgttatatt tttagattta gccaatttat aaactacatg
    44641 ccctgctcct ttacctataa gacctctttc cataacccct tctaaaatag gttttacttc
    44701 aagagaagaa aatcccattc tcaacaagac agacctttct attgatggtg ttgtattctt
    44761 tttaccaagt tctatcattg gttctactat ctgggtagct aaactccaaa acctttcttt
    44821 taactcatca tcactcagtt cttgaagatg ttttcttcta acttcaaaat catcctctct
    44881 ttttttcatg aacattcctc cattcgtatt gatttaatcg aattttctga atttttattt
    44941 tatattaaat taaaaaacaa taattactat aaattattta atacatcaac aacaaaatct
    45001 cttgaacttt ttacttcttc cattaaaaat tctatatctc gattacttag cttatcaata
    45061 ttatcaatgt gattattaga tatacaattt tttataaatg actttcttat tttattcata
    45121 tcaatatcaa cacatttcaa taattctggt ctttctggta atattaagtt tactcctgca
    45181 atttcttctc ttggatttcc aaattttata tctattccat tttctcttgc aaaagtaagc
    45241 tgtggacata tatgtttacc tgcgcctgta tattctgttt cttgtaccac tataacttta
    45301 tcctcatcta actcttgagc taaagagaat gctgctgcta aagatgtatt tcctgctgga
    45361 cctctttcta taccttctag ctgtgccaat aattcagttg tataaaaaac ttctccttgt
    45421 tttactaata catatctatc catatatcta agtggtctag ctgcagacct aggtacatct
    45481 gacctatcag gccatgtaca gtatggtatc ccaaaacctg tatgtcctgt tgtaaatgat
    45541 tttttattaa attgattatc actagccatg tgtaatcctt ttaaatctac acttgctcct
    45601 actactttag tattaataga accagctttt atcaatcctc ttgcagttcc agtaagatta
    45661 ccaccacctg catttgtaca aacaactata tcagggtctt ttttatactt ttctctaaat
    45721 tggatagcta actcataacc taaagtttct acacctgcaa ttccaaatgg tgaatataat
    45781 gatgcgttaa aatagcctgt ttcttctaat aaaactaaga acgtataaaa taactctggt
    45841 ccaacagtta actgaacaac ttctgctcca tatgcctcac attttcgtgc tttttctata
    45901 atttcaggtt gacctacacc attagaatca taacattctt gaactattat acattttaat
    45961 ccctgcattg ccgcttgtga tgctacagct gcaccatagt taccactagt agctgctatt
    46021 acacctttat atcccatttg tttggcatga tatactgcag tagctgctct tcttgcttta
    46081 aaactaccag atgcattcat tgcttcatct tttacaaata ttctagctcc tttaccttct
    46141 ttagcacatt ttctagctaa tttagtcaaa tttttaagct ctattaaagg agtatttcca
    46201 acagcatact gtgattgaat tgcttcaata tcttgtaatg tatatcctgt ttcactcatc
    46261 attttctcat aatcaaaccc tattccctct tgttcaaaat aagaataatc caatccgatt
    46321 gcatttttca ttatttcgtt atttcttccc ataacagctt gataactcat atctttcatt
    46381 gatgttgaat tattcattat atacacctcc aaataatatt tctttagctt ggattcctat
    46441 ttggagtaac tctggtatac atttaccata atcatgagta taatatggat ttattttaag
    46501 taaattacct tttacaagtc tgcctgtaat tgttttaact tctactaagt cgcctataga
    46561 agcatcactt tgtataaaac ctttaaccca catttctaag gatactttct tagtatcctc
    46621 tggaacttgt ggagctcttt cttctggagt caatactata ttgtgtataa taacccaatc
    46681 attaatttta gcatccattt tttcatcccc ttattaattg actattgcct tactaattaa
    46741 tttactgtta ttttaaatta aacattattt taccaattag ctaactgtta tcttactaat
    46801 tagctagttg ctatcttact aattagttag ctgttatctt actaattagc ctttgattct
    46861 tcttccaatt caacttctat ctgatttctt atatctccca ttattgctct aggtactggt
    46921 atatccaaca tagttttaag acctggtcta gcatttatta ttaatggaat agagtttact
    46981 atcattgcat aagtacctac tcctccaggt atttcaggat ttatttgtaa atcaatgttt
    47041 ggtatacctt ttattgatac atagtctcct gttttaatac cttctgcttc tggtattatc
    47101 tgttgtggat gctccatttc aattaaaact tctccattta catatccata tccacattgt
    47161 ctacaaccag caacatttcc agccaatgcc tctccgtact tagattttct atctactgta
    47221 gtcattattg gctcttttgt ttgttctatc ttatctaatt tccaacctaa tccatcagct
    47281 atcatattta ttgattctac aaaacctaca tgacctgcta tagtcttatc ttctacacct
    47341 tttataaatt cttcccttgt tactccaaca ccttgttcct ccataacaga ctttccaaat
    47401 ggagataaat catttactct cttagctttt atactagtaa cttcttcaca agtgcctgaa
    47461 agcgccaata ctagtaaatc taatacaaat cctggattta ttcctgttcc aagtatactt
    47521 actccatttt cctttgcaac ttcatccatt tttttagcta aatctgcatc atcagcttgt
    47581 ggatatgcca tttgttctgc tgttgatata acatttattt ttctatttaa taagaaaatt
    47641 attttatcaa aagccttttt agtaaatgaa tcagttgcta acaaaacaac atctacactt
    47701 ttttctctaa aaacttcttc ataattttta ttgataataa cttctggtct ttctcctctt
    47761 tcaattccta aaacattata catacttttt cctgaagtac ttctactaca aacagatact
    47821 atttctatac cttctttctt tagaatcata ttagccatac ctattcccat agctccaaat
    47881 ccccatattc ctactcttac ttttctcata tatacaccct cctaaaaatt tatatattct
    47941 caataattag ttaaagcata aatcgtgcca actttaaaat taaagtaaat atttattgaa
    48001 acgttgaaac tataattatt ttattatgta ttttcataaa taaaaaataa tatttcttat
    48061 gagaaataaa aaaaagagca aaatattttg ctcttttata ataattttgc tcctctattt
    48121 tgctttttta ttttactttg attttgtttt tttatcttgc ttatttataa tattttgata
    48181 ttatattttt ttattttata ctgtaaattt tgcctattca tacctaaaat tgatgcagct
    48241 ttagttatat tgtaagatgc cttttctaat gattcatata tatacttttc ttctatctca
    48301 ctgataatct tactaagtgt tttgtcctcc aaatcgttta aagaataatt gttactctct
    48361 ttattctctt taagattgta atagtcgttt agattaatca tacttgtttc tatttgtacc
    48421 tcatcttcca tcatactcat agtacaataa attatgtttt ctagctctct tatgttacct
    48481 tgccaatcgt aattttttaa aattttaata gcttcatctg aaattcctac tactttttta
    48541 tttaactttt tattatactt tgaaataaat tttttgcaca atagaggtat gtcctctttt
    48601 ctatctctaa gaggaggaat ttttatatga attatgttaa gcctataata taaatctttt
    48661 cgcattttat gctcgtttaa aattaccttt ggactttcat ttgtcgttgc aataattctt
    48721 acatctatag gtatatcctt agttcctcca acccttctta cataaccttc ttgtaaaacc
    48781 cttaatagtt tagcctgtaa cataataggc atggaattta tctcatccaa taacaatgtc
    48841 cctttgttag cttgttcaaa taagcctggc ctatctattg ctcctgtaaa tcctcccttg
    48901 ctagttccaa aaagaatacc ttcgaatagt gattccggca aagctgcaca attttgtgct
    48961 ataaatggat agtttttcct attacttcca tagtgaatgg actggctaaa taactcttta
    49021 cccgttccag tttcaccgta tataaatact gaagcatcag acttgcaagc tttcttagat
    49081 ttagctattg cttctttaat caaattactt tcccctaaaa tatcatcaaa ttcataaccc
    49141 tttttttgtc taatactaac tttattgact aaattttttt tataatttct gtttagtaaa
    49201 ttttctatcg ttgtcatatt ctttgatatc tcaagtgccc ctacaaactc gcctttaata
    49261 tcaattggta ttgtagtatt aattgctgtt atctctcttc catttttatc cagatatttt
    49321 tgaatattgt ctttgataac ttcccttttt tccaaaactt ctaatagtgt actcctgttt
    49381 attttcattt tgttgactat atcaaaaaat gaattggtta aaatatcgtg tctgttcatt
    49441 ttttccattt tttgtacaga attattgtag attatagttt tcccgtcttt attaacaaaa
    49501 tgtacccctt catcaatatt ttccaatatt tcattcatta ttttaaaaaa tatctcttgg
    49561 ttcaactata tcaccccagt acctaaatat ataaaaaccc aaaaaattaa taaaatgttg
    49621 ctattgtatt tatcttatca tatatttatg atatttttta tgtacattat attaatatat
    49681 ttttaagtat atttaaaatt ttatttatat atctttcatt atggttatat acaatatttg
    49741 a
//
LOCUS       DAYAPA010000014        48048 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.014, whole genome shotgun sequence.
ACCESSION   DAYAPA010000014 DAYAPA010000000
VERSION     DAYAPA010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 48048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48048
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.014"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            76..774
                     /gene="yunB"
                     /locus_tag="ACGI59_000709"
     CDS             76..774
                     /gene="yunB"
                     /locus_tag="ACGI59_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YunB"
                     /protein_id="HFG9738515.1"
                     /translation="MNRIVDRKTKNEFRKIIAIFLVILFLSVFIGSFIYIDKTLRPTI
                     TVLAETKALELANRSINKAVAEMVEGKINYEDLMDIQLDNNGKITMIQANTIMMNEIA
                     SAIALEIQDELKKDKTASSYIPIGTALGSPILAKYGPKLEVSIEPIGTVSVNFKTEFE
                     SSGINQTRHRIYLEAQTQVKVVIPLITSTKQIKAQIPICETIIVGDVPESYVNIPEKN
                     LGNVLPNTGKNTNK"
     gene            complement(986..1993)
                     /locus_tag="ACGI59_000710"
     CDS             complement(986..1993)
                     /locus_tag="ACGI59_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIVB peptidase S55 domain-containing protein"
                     /protein_id="HFG9738516.1"
                     /translation="MQLKNFHFKNNTLSKKTNLAKSKFTIVLTFLFLLYFFSNNLIYA
                     QNLQKNEPEYLIPIGNVLQIDAELKNIIVRNPGEASPFRLGDAIVKVNNAEVDGYSDF
                     ANTLNALPEEKQVSILLNRGGQLITMKTTKHILEKISFNNLLSGFATLTYINPDTSEF
                     GAVGHPISVGNARKIPIKTGSISTTKHLNIEKSYKGSVGCINAKRENIIGSFTENTDF
                     GIRGQINNFDISKLKKYKVASLDEVKLGKAQIILQDNFNKCKKYNIEIIDIENQKHPE
                     SKSFKIKITDKKLLAQTGGIVQGMSGTPIVQDNKIIGAVSHAIENDPAVGYGVFIKWM
                     L"
     gene            2221..3105
                     /locus_tag="ACGI59_000711"
     CDS             2221..3105
                     /locus_tag="ACGI59_000711"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFG9738517.1"
                     /translation="MKKYRLLVTCIIAMGIIIIGTSNPVSNFYKTTNLISSGNITNEK
                     ADKDENSNKNNKDKNEDKSNSKSNKDKSSEDKSSDNKNEIQKNKKLLICIDPGHQGKG
                     DSNLEPVAPGSSSKKARVSSGTEGIATKKPEYVLNLEASLVLKSILESKGYNVIMTRE
                     THDVNISNSERAILANDKKADMVVRIHADSLNNSSKTGASILIPEKDGKYTAPIYEES
                     NKCAEFIKQNMEQSGIQINGIVQRGDLTGFNWSKVPAVLVEMGFMSNYNEDQMMSNPD
                     YQRKMMQCIADGLDAYFK"
     gene            3307..4857
                     /locus_tag="ACGI59_000712"
     CDS             3307..4857
                     /locus_tag="ACGI59_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007788866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFG9738518.1"
                     /translation="MLVVENVSHGFGARTILENVSFRLRKGEHIALVGANGEGKSSFL
                     NIITKKLMPDAGNIKWSSRATVGYLDQHTVLSKGKTIREVLREAFKHMFDLEQEMIAM
                     YDKMGEASDDEMSKLLEETAEIQTILENSGFYMIDAKIQEVANGLGLGEIGLDKDVTD
                     LSGGQRTKVLLTKLLLENPTILILDEPTNYLDEEHITWLTKYLQEYENSFVLVSHDIE
                     FINNTCNVIYHMENGELNRYKGNYDEFVRLNDIKKRQEEQAYDKQVEERKRLEDFVAR
                     NKARVATRGMANSRQKQLDKMEILERPKEKIKPTFAFKDARAASKIIFETENLVLGYD
                     EALTKPLNFHLERGKKIALKGMNGIGKSTLLKTLLGIIKPFEGNVKLGDYLEVGYFEQ
                     ESSRENSNTPMDEVWSEFPGLTNFEVRQALAKCGLTNEHITSQMRVLSGGEAAKVRLC
                     KVMLKNINFLVLDEPTNHLDVEAKDELKKAIKEFKGTVLLVCHEPEFYSEIVDDVWNI
                     EDFTTKIV"
     gene            4886..5533
                     /locus_tag="ACGI59_000713"
     CDS             4886..5533
                     /locus_tag="ACGI59_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HFG9738519.1"
                     /translation="MKKFLNVLFSICISIVIIVGVINFTVGFKQLYYFDIDYLNISEL
                     SGLSKDDIKLNYDYLIDYNLNKNVSEFKLPTLKSSPQGKIHFEEVRNIFQNINKLAKL
                     LLVVSLVGIILSVKNKNIKILKTTSITLIIMPLLLTVPILLNFEKSFIIFHKLLFRND
                     YWIFNPDLDPVINILPEEFFFHSGMMILILILLVSIVLFVMYKLCKSLSIKIFQC"
     gene            complement(5675..5935)
                     /locus_tag="ACGI59_000714"
     CDS             complement(5675..5935)
                     /locus_tag="ACGI59_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich small domain-containing protein"
                     /protein_id="HFG9738520.1"
                     /translation="MGENYKFFNHKDCEFFPCHKTNKPEEFNCLFCYCPLYALGENCG
                     GNFRYTDKGIKDCSSCILPHKKDNYNYIMSKFQDLVKITSKK"
     gene            6152..7435
                     /gene="hflX"
                     /locus_tag="ACGI59_000715"
     CDS             6152..7435
                     /gene="hflX"
                     /locus_tag="ACGI59_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HFG9738521.1"
                     /translation="MKEIQEKALLVGLNLTTIVKKNDDIDTNESMEELKELTKAAGAE
                     VVGSLIQNKHSVDAAYYIGKGKVEEIRAYSDSLDATLVIFNDELSGAQIRNIENVVGR
                     KVIDRTTLILDIFAQRALSKEGKLQVELAQLKYRLPRLYGMGGEMSRTGAGIGTRGPG
                     EQKLEIDKRHILNKAADIRRELKEVKKNRETQRVKRLKSNIPIVALVGYTNAGKSTLL
                     NELIKTHKDYEQEKEVFVKDMLFATLDVTLRKALLPNKKEFLVVDTVGFVSKLPHDLV
                     EAFKATLEEVQYANLILHVIDATNTSYELQKSTTEGVLKELGVNDKKHILVYNKVDKL
                     ELDIYPKSQEDIVYISAKQGINMDKLLNMIEIALMENTYSVSLMLPYERGDIFSRIKD
                     KYNVENFEYGESGITLDADLDEEDFNIYREYILEK"
     gene            7443..8120
                     /locus_tag="ACGI59_000716"
     CDS             7443..8120
                     /locus_tag="ACGI59_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HFG9738522.1"
                     /translation="MDINWENIDNLEDYFITYLLYKESKTVSQISKIRNISSTEASEQ
                     LIQAKLKIKEMQKDDFEASKDILDKFLELDKIKRLDFMDSLDDEKMVYFKRKVFKRIL
                     VEKNAEDLIVLIWATGELKDDRFLKLLHQLTNHRHSDIRRITYSAIRKIESPSSREVL
                     QKGLYDKNAQTRQYCAKALSKLGDENSLKILQQLKDKNKNFEKEYVLRAYDEAIKSLE
                     NAKNLKK"
     gene            8206..8649
                     /locus_tag="ACGI59_000717"
     CDS             8206..8649
                     /locus_tag="ACGI59_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFG9738523.1"
                     /translation="MIVRRCAGGVVFYANKVLIVKNDRGEWTLPKGKILGGGLPYESA
                     VQRVKVETGIDAKMIDVAGDTMYEFFSRSRQQEVCNAIMWYVMEACNTDCVLAPEFQE
                     GGFYKVKDALEMLSHHKEQALVEVSYKKFKELKKLVSEGDDVPAN"
     gene            8954..9598
                     /locus_tag="ACGI59_000718"
     CDS             8954..9598
                     /locus_tag="ACGI59_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HFG9738524.1"
                     /translation="MSNYRTLHEFGTDEITIEKSVFIGYAKPIKSEEEALEFINEIKK
                     KHKDATHNVWAYTVGKNMNIQRYSDDGEPQGTAGIPTLEVIKKEDLRDVAVVVTRYFG
                     GTKLGAGGLVRAYTKGAKLGLEAGKIIYKVMYQEVKVKIDYTQLGKVQNELMNLGYFI
                     KDTVYEDNVEIVVYSRLEDVEKLSEKMIDITSGTGKIVLGEEFYLSEQNGEILI"
     gene            complement(9684..10061)
                     /locus_tag="ACGI59_000719"
     CDS             complement(9684..10061)
                     /locus_tag="ACGI59_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HFG9738525.1"
                     /translation="MLFALIGFFAGIIGGMGMGGGTILIPALVLLAGIDTKIAQSVNL
                     LSSIPMTLIALAIHIKNKNVIFKLVIPIAVFGVLGAVFGSLLANYLSSDILKKIFGIF
                     LLLVGLFEVKKGFCAKSDKKPLN"
     gene            complement(10061..10447)
                     /locus_tag="ACGI59_000720"
     CDS             complement(10061..10447)
                     /locus_tag="ACGI59_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFG9738526.1"
                     /translation="MKKTDDLKKKYFDFNLKNTIIGVFTGFINGVFGSGGGTLLVPIL
                     NDIVKVEEHKSHATALSIIIFLTTASSVLYVSKGTYDVNLTIKVAVGSILGGIIGAKL
                     LNKVTGKFLRISFGIIMIIAALRMVF"
     gene            10626..11381
                     /gene="nadE"
                     /locus_tag="ACGI59_000721"
     CDS             10626..11381
                     /gene="nadE"
                     /locus_tag="ACGI59_000721"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HFG9738527.1"
                     /translation="MSNIKIQIDKTVEWLINKVNEANAKGLIVGVSGGIDSAVVANLI
                     KKAFPENSMGVIMSIKSNPQDREDALKVIEGCDIEYLDLDLIEPQSAILDMVVGNLKD
                     KHLYREEYLKMTDANLRARVRMSTIYTIANNLGYLVVGTDNAAEIHTGYFTKFGDGGV
                     DILPIANLTKGEVYEWAKELGVHEDLINKAPSAGLWEGQTDEDEMGTTYNMIDAVLEG
                     RLDEVPKRDQEIIERLHRLSEHKRKTPAQPPKF"
     gene            11464..12183
                     /locus_tag="ACGI59_000722"
     CDS             11464..12183
                     /locus_tag="ACGI59_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFG9738528.1"
                     /translation="MGRIGNIINRKGKQDAQRAKIFTKHARAIAVAAKEGGADPEYNA
                     ALKTAIEKAKADNMPNDNIDRAIAKGAGAGAGEDYETIVYEGYGPGGVAVIVETLTDN
                     KNRTAGNVRYYFDKNGGNLGTSGCVSFMFDKKGQILVGLGDGVSEEELMDVALEAGAE
                     DFITEEDGYEIITTPEDFSSVRDELKAKGYEFISADVKMIPQTTTVLTEESHLKMMNK
                     LVDMLEEDDDVQDIYHNWEVE"
     gene            complement(12350..13354)
                     /locus_tag="ACGI59_000723"
     CDS             complement(12350..13354)
                     /locus_tag="ACGI59_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFG9738529.1"
                     /translation="MATIKDIAKDCGLSSATVSYVLSGKGNERRIPLHTQELILNSAD
                     KLGYKRSTPIKHNNIKIAFYWQDEGFELASTNILQAINHIISLEHLNINIIIRPYKVG
                     HLCDDEYFFANNYYDGIVLTGINADDIEYLVKNKPKIPVVVVNREIPGFSSVSIDNEE
                     TGRIAALHAIQNGKNDIAVVFNPTSFTCMTLRGKSFLKTCKEHGIHLSDSIYYCSNKI
                     DSGYDIAQKMLAKNSLKKVIFCNHDVVALGMMNAFHESGIVIGEDIQIFAASNGPSRL
                     MARSIPPLTVIDLKMEETAERSLRMIIDTVTGRINGIQQQVIYPSIIYRQSSPAQNLL
                     "
     gene            13620..14516
                     /locus_tag="ACGI59_000724"
     CDS             13620..14516
                     /locus_tag="ACGI59_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HFG9738530.1"
                     /translation="MSKVNIFEVGPRDGFQNLKEFLETDKKLKIIDGLIASGIKQLEI
                     SSFVSPKAIPQMKDAKEVATYCVEKYPDVKLYALVPNLYGAKVAWECGIRDISYVISL
                     SETHNKKNINRTHEESIDELKKIMNTYPDMNICIGMATVFGCPFEGLPKIEDAIRFAK
                     RLWENGIREINLADTIGIADPKQVRETISALVEALPECRWQVHIHDTRNMGMVNTLVA
                     IESGITTVQSTLGGLGGCPFAPGASGNTATEDLVYMLERMGYDTGVDFVKLLELSKYQ
                     KSFVNGVYSGHHVNIKSDSCNC"
     gene            14637..15899
                     /locus_tag="ACGI59_000725"
     CDS             14637..15899
                     /locus_tag="ACGI59_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFG9738531.1"
                     /translation="MDIIALLLFLGVIIIAFVRKNNAGILALAIGVIAVRIFGMDDKE
                     LYGAISNSMFTTLVGITLLFAIVNSTGALKLVAEKIVAASGKRVWLIPIAVFIAGFVV
                     AGVGPGAIPALAIIPAMAVPVAITVGYNPVMLALIGECGLMAGRMTPITPEGQIITTV
                     AEGVGIENVMPTILACQTLSTIVFSIVLFIIFKGYKLKKPINVIDSKQIEKFNIKQII
                     SLISIVVMIAFIIIFDMNVGLAAFAVSAVLIFFNIADEGECIKSIPWSTIVMVLGVGA
                     MMTIVDAAGGIDLMSNALSSLMNSKTATPIMSISAGLMSMVSSALAVVYPTMMPMCVD
                     IAKAVGGVDPLALIAAVGVGGSLAGVSPLSTGGALILAAMGSSKKDFNKEEQNKVFVQ
                     LFIMSAVGLLVIAVVSILSFNAITHLMN"
     gene            15975..17168
                     /locus_tag="ACGI59_000726"
     CDS             15975..17168
                     /locus_tag="ACGI59_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HFG9738532.1"
                     /translation="MKKGALSNIKVLDLTRVLAGPYCTMMLADFGADVIKIEMPRKGD
                     DTRGFGPFVNGESLYYANINRNKKGITLNLKSEEGKNIFLEMVKHADVVVENYRPGVM
                     EKLGLGYDELAKVNNQIIYGAVSGFGCYGPYSERPGYDIIAQAMGGLMSITGQEGGEP
                     TRVGNAMGDVLGGMNLTIGILTALNARKIIGKGQRVDVSLVDSVVSSLETGIQRYFDS
                     GQPELMGNRYASAYPYDSFKASDGSFVIGCGNDKLFEKLCKKVLFREDLLIDERFKTN
                     LNRCEHYKALKPEIEKWSVKHTIKEAVDLISEAGVPAAPIYNLDCVTTDEHIAKAREM
                     IVDISHPIIGNMKVNGNPVKLMTTKAKIDMPAPSLGESNEEIYNGWLGFDSDKIKDLK
                     ENGVI"
     gene            17797..18591
                     /locus_tag="ACGI59_000727"
     CDS             17797..18591
                     /locus_tag="ACGI59_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HFG9738533.1"
                     /translation="MESVMENLNNLKVEVKDKVCVITINRPKALNALNSDTLRELSQV
                     IDVVSENEAILGVIITGEGKVFVAGADIRQMQNYKSEEGRKYAGYAQGIFDKIEALEK
                     TVIAAVNGYALGGGCELAMSCDIRIASEKAIFGQPEVNLGVIPCFGGTQRLSRLVGTG
                     IAKELIFTGRQVNAEEAKSIGLINKVVPSDLLLEESMKMMNQIVEKAPIAIRYAKVVI
                     NKGIDMDLKNALELEKDVAGLTFATRDKQEGMNAFIEKRKPVFENK"
     gene            18686..20026
                     /locus_tag="ACGI59_000728"
     CDS             18686..20026
                     /locus_tag="ACGI59_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HFG9738534.1"
                     /translation="MNTGVIISLVGIIIALSLLVVMVMKGINIFIIAIVCSSLVAITG
                     SLNVYDALKVDYMTGFVGFLQANFFIFLTGTLMGKIMEITGGAKSIAKMIVRWIGKDK
                     ALLSIPIACGILAYGGVSVFVVSFAVFPIALEIFKEADLPRRFIPAALTFGCSTFAMV
                     APGAPQIQNIVPASTLGTDIMAGAVNGFISCAVMFIVGSTILYKMVSKEKANGGHFIA
                     HESDIFDEAEATNSKRENGPNGVIALIPLIVSILIINVKVNGKAIVPIEVGVFIGALL
                     VYLLLNKYQDNTKIVGHVGDACKTTVVAICNTCAVVAFGSVVKSAVGFDFIVNAMTNI
                     PGPPIAGAAVGTTVIAGICGSASGGLGIAAPLLGPVFLAKGVSAAALTRTMAISSAAL
                     DSLPHNGYIVTVTNGLCKETHKDAYMPIFWVTVLTPFIGTIVAVLLFTMFPGLP"
     gene            20163..21395
                     /locus_tag="ACGI59_000729"
     CDS             20163..21395
                     /locus_tag="ACGI59_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HFG9738535.1"
                     /translation="MLNKPLEGVKVIDLTYFVAGPGTSKILADWGADVIKVEPSFGDP
                     GRKTGATMTMPIDDYNNPFYSTYNSNKRGLSINLKSETGIEIMDKLLSEANVFVSSYR
                     TGALKRLGLDYESLSKKHPHLIWGQINGFGDFGPAKDNAGFDTVAFWARSGAMLDIAE
                     KDTSPINPPIAFGDATTSCSLSGGICAALYQQAKTGKGQKVMASLFGQAIWNASSLLA
                     STQFSDEYPKTRKNAISPVINSYRCKDGKWIFLSILEHERYFEALCRLFGREDLIKEE
                     KFASSVESKKNATELINILDGEFAKFTQNEMVEKLTAADIAHEKIQHVNDVVTDEQAI
                     ANNYIYEHTSKDGNKTMLASTPVKFGNIEVNMTCDAPLIGEHSDEILRELGYSDSDIE
                     TLIESGIVTMNKEACEIC"
     gene            21435..22577
                     /gene="acrC"
                     /locus_tag="ACGI59_000730"
     CDS             21435..22577
                     /gene="acrC"
                     /locus_tag="ACGI59_000730"
                     /EC_number="1.3.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016628 - oxidoreductase activity, acting
                     on the CH-CH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0043958 - acryloyl-CoA reductase (NADH)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acryloyl-CoA reductase"
                     /protein_id="HFG9738536.1"
                     /translation="MDFRLTEAQLMLQRVAKEFAENEIAPIAAETDKTGIFPRELFSK
                     MAKIGFNGIGTPVEYSGSGGADIEKVIVVTEIAKKCAASAAILSIHTIYAQAILKFGT
                     EEQKKKYLPMMAEGGCVGAFALTEPNAGSDAARAATTAIIDEETDEYVLNGTKCFISG
                     GGQAESLIIFALTDPSKGIKGMSAIIVDKGTPGFSIGKIEEKMGIHGSETAELIFDNC
                     RVPKSNLLGKEGKGFNIAMTCLDGARIGVGAQAVGIAEGALEESIKYSKERVQFGKPI
                     SALQGIQWYIADMATMVESAKLLVYYAADLKARGEKHTKEAAMAKYNASRTAREVTNL
                     ALQIHGGYGYMKDYPLERMYRDAKITEIYEGTSEIHKVVISRAVLG"
     gene            22588..23382
                     /locus_tag="ACGI59_000731"
     CDS             22588..23382
                     /locus_tag="ACGI59_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta"
                     /protein_id="HFG9738537.1"
                     /translation="MNIVVCLKQVPDTNEVKINKETGTLIRDGVPSIINPDDRNALEE
                     ALKMKDELGAVIKVISMGPPQAKSALKEALAMGADEAYLISDRAFGGSDTWATSTIIA
                     AAIEKVGKYDVIFCGRQAIDGDTAQVGPEVAEFLGIPQVTYAKEVKVQDDKLLVTRYT
                     ETGDYLIEAKMPVLLTAIKELNNPRYPSVKGILEAYNNGDAKITVLTLADLDVDTTQI
                     GLKGSPTNVYKSFVPVKDKHNEIIEGINKKEKAEKLIEILFDLKLV"
     gene            23396..24415
                     /locus_tag="ACGI59_000732"
     CDS             23396..24415
                     /locus_tag="ACGI59_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFG9738538.1"
                     /translation="MRAKVNQGINLNDYNGVWVIGEQREGKINPVTIELIGEGRKLAD
                     QLGKELAVVIAGYEVEKEVKELLHYSVDKVYYINDPLLKDFTTDGYAISIANLIERKK
                     PEVVLVGATSIGRDIAPRIAGKVGTGLTADCTKLEIDSTDNKLLQTRPAFGGNLMATI
                     VCPKNRPQMSTVRPGVMAKAVRNESETGILEVVTPELTEKMIRTRLVEILPQEKKSVN
                     LTDARIIVSGGRGLKRAEGFELIKELADKLGAEIGASRAAVDSGWIEHSHQVGQTGTT
                     VRPELYIACGISGAIQHLAGMSDSKYIVAINKDAKAPIFSICDYGIVGDLYEIIPEMI
                     ESLNR"
     gene            24514..25890
                     /locus_tag="ACGI59_000733"
     CDS             24514..25890
                     /locus_tag="ACGI59_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 interaction domain-containing protein"
                     /protein_id="HFG9738539.1"
                     /translation="MKEQWYKDIFARVLSMTDDGFIVVNTSGVIIDINDKYCDFLGKE
                     RKDIIGQNIQSIIPNTKMLDVMKNKYCEEGAIHHYSGGNTKEKSVIVSRSYVENDNGE
                     VVAGVAQVKFRLQSFDVAKKLMSEYMELQYYKEQFKDNCGFDKLIGENRDFIELKKTG
                     VKASKTNFPVLLTGETGTGKEVFARAIHNNSSRSDKPMVSINCAAIPEELLESELFGY
                     DEGAFTGAKKGGKKGKFLVANNGTIFLDEIGDMPLTMQAKLLRVLQESEIEPVGGLKT
                     IKIDVRVISATRKNLSKMVEEGLFREDLYYRLNVINIHMMELKDRQDDILLLANYILN
                     KLNVEYKELKVLSDKVKNCFINYTWPGNIRELQNVIKSAYAVSDDMVIMMCDLPSKMD
                     NISRVAQCNVDSNCSIHEMVENYEKSLIIDVLRKYNWKCSKAAEVMGIHKSLLYKKIK
                     KYEIELNN"
     gene            complement(26222..26566)
                     /locus_tag="ACGI59_000734"
     CDS             complement(26222..26566)
                     /locus_tag="ACGI59_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9738540.1"
                     /translation="MDQIKRLHELQQKSYLTKEEFEELKNNTYIERFELRDECDNSYI
                     YTFYTNDKCNDIKFIKSQFIVTLIKIGFLCECGGVFRQKKIVDEYDYGYNAIYKCDSC
                     GKELDKEVENYD"
     gene            27054..28064
                     /locus_tag="ACGI59_000735"
     CDS             27054..28064
                     /locus_tag="ACGI59_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HFG9738541.1"
                     /translation="MHEDKNDIFESIDTEDFDNKTEEECLELVRKNGLNLIYIKNQTE
                     EICLEAVRQNCNAIKCVQNQTEKICIEAVKQDWRMLEFVEEPTEEICMLAINQDGTAL
                     KYIENQTEELCLRAVEKNGAALEYVKEQTEEICIEAVRNSEFGLARVKNQTEKICMEA
                     VKHCSYNLKWVENQTEEICMEAVRQNGLDLKFVKNQTETICLRAVRQNGMALEFVKEQ
                     TVGICLKAVRQYGMALKFVKEQTEEICTEAIKQDKRALSFVKGDKEKYKALYDNNEPF
                     AKRYVRNVIEKENRALIKKGEENAKIKIAGKLYDRGMSFEDISDIVEIEISKLKVSLG
                     VF"
     gene            28200..28688
                     /locus_tag="ACGI59_000736"
     CDS             28200..28688
                     /locus_tag="ACGI59_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HFG9738542.1"
                     /translation="MKITVLYQTRSGNTERVAKLIEEGAKKVDGVDVKLMRLDNIDLD
                     YLNESKAVIFGTPTYLANTTWEVKKWFDEDSKKVNLAGKLGAVFATCDYICGGPDVAI
                     LTIVGHLMVKGMLVYSGGGSLGKPFIHLGHVHSIEGPELQDEKAIIFGERIATKAKEL
                     FA"
     gene            28864..30792
                     /locus_tag="ACGI59_000737"
     CDS             28864..30792
                     /locus_tag="ACGI59_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFG9738543.1"
                     /translation="MVWHIEYEIFSAIIVIFLMVYFFRSKFIPTLQNKIYCALLIFSF
                     LFIISNILGSFCLNNIDKIPIFITFLLNQIYLLLLPIPAALVSFYVMAIIYQDIRYMK
                     KRLLFLSIPLIICIILSITNPFTHILFGLSKETGYIRGCGYVATFASFFFYAIYSAFL
                     SFKYRKAMHESKIWAIRGFLIVSIVAIILQAIFLQYLLTGTACACSLLLTYLSMQNRG
                     LIIDDLTGVLNRQSFIQELDLNINTEQSGFIITIALDDFKFMNETFGTKNGDIALKEV
                     GKYLIQIHDVNHVYRIGGDIFSIVVNSQLGIEPDDIIRKIEERFKKPWLVEEISFNLA
                     TSIAVVYYPENAETTEDVIVAIDFSIHEAKRSESRRVVYADASISEKIKKKHVIKNCL
                     KEALKNDGFEVYYQPIFSNSRGRFTSAEALLRLEHKELGFIPPDEFIPIAEQTGLINS
                     IGLVVFEKVCRFIASDEFEDLKLDNIAVNLSVVQCMQKNLADDLLYLMNKYNVSPTKF
                     KLEITETVAMGSFNIIKETMERLIDLGVKFALDDFGIAYSGVTNMLSLPFSVIKLDKS
                     LIWSMNEDSRHKLTVETIIALINKLNMKAVAEGVETIKCAEDLIAMECEYLQGYYFSK
                     PVPEDVFKNLLKENTFTS"
     gene            30969..31379
                     /locus_tag="ACGI59_000738"
     CDS             30969..31379
                     /locus_tag="ACGI59_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFG9738544.1"
                     /translation="MNKKKILVPIDGTERSMHSLEFIKGIFKKDEVEVEIMNVKELVF
                     IDGISLAEEIKNSENLGRRILDKAAEIMGEYDVKVHFTFGYPGDEIIRKAKEDNVDFI
                     VMTKSTKKGLTRMIGSVTASVVKQAKCIVMIVPE"
     gene            31401..31703
                     /locus_tag="ACGI59_000739"
     CDS             31401..31703
                     /locus_tag="ACGI59_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIBC"
                     /protein_id="HFG9738545.1"
                     /translation="MNKKIVAVCESERSLNSLKTAANDLGFDLVYEIQENDNIKNELS
                     IYDIEDASIVLFVTSDSIESIEKIDRFIDREFYEVDPKYIIEDAKSIVSEILIDLN"
     gene            complement(31920..32612)
                     /locus_tag="ACGI59_000740"
     CDS             complement(31920..32612)
                     /locus_tag="ACGI59_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HFG9738546.1"
                     /translation="MKSLNATEKIIDYIKLNILNGTFKINSKLPSERKIADLFNISRI
                     PVRNAINILCKEGILRAVPYSSPIVEGFKKVDLFDDGEIYKNHNIQEFYVESLRARQL
                     IESEATKLAILNATSKELQKIKYTYLKSIEELDKVSHGLIEECYDADLQFHKEIILAS
                     HNPIFIKYYELIPKTVSSNQYFGFKYRNSLKDMISHHNKIMIAFDSKDSYLGYTSMYN
                     HLEDVIQLFQHD"
     gene            complement(32638..33825)
                     /locus_tag="ACGI59_000741"
     CDS             complement(32638..33825)
                     /locus_tag="ACGI59_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFG9738547.1"
                     /translation="MKKISNQKLLLLSIVTFFWFSQYVYIPFQNPYLSACNVSTNMIG
                     IIIGAYGISQCLLRLPVGVFADSINKHKIFILSGIFFAGLASLIRVFSPGGNGFLIAN
                     ILSGISSSTWISYMVHYTNFFSTEHQQKATSTIILVNNLGMLIGFVMSTLFYEKLGMT
                     ALCIMSVLSSVIGTILALNIKNHNSKKIPEKISIKKYLSICKDKRLVFFSFMALIQQG
                     IQMSTTMSFTTQILKNLGSSSTLIGLSSIFYMVCAVASSGFASTKFCTKYGPKFWIPL
                     VFILVSAYCFLVPTIKNINIIFVLQVLPGISTGILLSYLTSESMKNVPKHQKSTAMGF
                     FQAIYAIGMSVFPMIVGKISEYTSIQYGYFILAIIAILASILSYTYYKKVDLTLNYDI
                     IQS"
     gene            34161..36038
                     /locus_tag="ACGI59_000742"
     CDS             34161..36038
                     /locus_tag="ACGI59_000742"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFG9738548.1"
                     /translation="MDYKNIAQEILLNVGGKENVNEVTHCMTRLRFKVKSASKVNKDK
                     LSKTEGVITVVESMGQIQVVIGNKVKKVYDEVIKIVPQSNNIGKKNESQEKQGIINSI
                     LSAVAGIFTPTIPAIAGVGMIKGILSVLAMYYMNKNGVDIKETQSYIILNAMADSIFY
                     FMPIILGYTAAKVFNANKIISMVLGATLCYPTFTALMAGEESVRFLGLAVTKATYTSS
                     VIPIIIAIWALSYVEKVLEKYIPEVIKIIMVPTLSLVVMLPATLFLFGPIGIYIGNVI
                     NFSYKYIYELSPALCGAFVGGLWCVLVIFGAHRALLPIGISDVAQTGRQNLLAFAGAA
                     NFSQAGAALGVFFKTKNQGLKTISMSATITALFGITEPAIYGANLRLKKPMVCAVICG
                     AFGGAIMGMGGAYGNAFANQGVLTIPVYAEAGALGFLSYLGGCAIAFFGSAISTYLVG
                     FEDLEESSNKENSSIKVETKDGVIDITSPVEGECIELSEVKDDVFASKAMGEGIAVLP
                     TKGVITAPTDCEVASLFPTLHAIGLKLDNGAEMLIHVGINTVELNGKYFTKHVNQGDL
                     VKKGDKLISFDIDKIKKAGYDVTTPVIVNNTFDFGQVVSCKSSYVSTNDNIISLVLAG
                     N"
     gene            36395..37801
                     /locus_tag="ACGI59_000743"
     CDS             36395..37801
                     /locus_tag="ACGI59_000743"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFG9738549.1"
                     /translation="MNTGFPKDFLWGASSSAFQVEGAWDKDNKGKTVADYNSFKKSHL
                     QADTKVASDFYHNYEEDIELMKELGMKTYRFSISWARIIPDGEGEINQKGLEFYNKII
                     DKLIECDIEPFVTLYHFDLPFKLVEKYNGWESRETVYAFERFAKICFKHFGDRVKYWQ
                     PHNEQNLIVRVEERINIYDETDSWKIDKMRAQMDYNLCLAHALAVNACHEMIKESKIG
                     AAVSSSVTYPLTSKPEDVYAARMNDNFKVYYMLDMHHYGEYPGYYMKYLEKRNIVPHM
                     EDGDKEILKKAKMDFIAVNYYRTNCAEALPEDSQHPFGLREGTVDFSMYGLFKMSMNP
                     NLEASEYGAAIDPSGLRVALNEYWQRYHLPVIITENGLGAKDILEDGKIHDDYRIDYL
                     RSHINACKLAIEDGVEMIGYCPWSFTDLLSSSQGFNKRYGLVYINRTDHEVLDLKRIK
                     KDSFYWYKEVIENNGIVK"
     gene            37829..38677
                     /locus_tag="ACGI59_000744"
     CDS             37829..38677
                     /locus_tag="ACGI59_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFG9738550.1"
                     /translation="MYITKVLNNSLLLAKDDNDEEIILMGKGIGHNYKAGSELNKKDI
                     EKIFVLHDENIKKSIIQLATEIDAEYFGIAQMIIAYGIEKYNLKLMNHIYLALTDHIA
                     FAVRRFKEGIMIENHYLFEIKEFNPKEYDIGKYAITVFKEVLGLDLPEEEIGNIAGHF
                     INAQQDNPYSDRNKRSAKIVNAILQIVHYHFSIVYNKESFYYRRFVMHLKAFSQRFLA
                     NEPSREKIDFIYEQVQKNCKEEYECVKKVGAYMYKEYSRELPRQEELYLMIHIHKILG
                     ELCENS"
     gene            39338..40030
                     /locus_tag="ACGI59_000745"
     CDS             39338..40030
                     /locus_tag="ACGI59_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFG9738551.1"
                     /translation="MNKVLIIDDDKDLCILLKKSISIEQIEANYCHSGRDGLVELSKQ
                     DYQLVVLDVMMPGMDGFETMEQIRKYSSIPILMLTSKSDNYSKVHGLRAGADDYLTKP
                     FEITEFTARVLSLIRRYTRFNPKNEEPQQLSFKGLLIDLDQHTVTTNGEPMLLPPKEF
                     GVLLYCARHQGKILTKQQIYEAVWGEPYVYDDSNIMAIISRLRKKIELDPSSPIYLQT
                     IKGIGYQFNTEV"
     gene            40027..40962
                     /locus_tag="ACGI59_000746"
     CDS             40027..40962
                     /locus_tag="ACGI59_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFG9738552.1"
                     /translation="MKMDEQTIILFLLLLSIGLLFLSIFAFSKLGHIYKRLKDIEEIL
                     ADVQIGDENRKILIKPCDVMAPLVYQLNEIVYDYENKLLSLKKSDKASKQLMTSLSHD
                     VRTPLTTLIGYLDAVHSGIVIEQEREEYLEIARRRAYDLKDYIDVLFDWFRLNSDEFT
                     LSIESVEIAELSRNILKDWIPIFHEKKLNFEIDIPENRLMVNLDPDGYSRVVNNLVQN
                     VLAHSKARQIKITMSEDSRMVLLRVEDDGVGIARENLPHIFERLYKCDKGRSEKGSGL
                     GLSIVSQMVERMGGQVSVESEIGKYTVFTVSLPLI"
     gene            41110..42033
                     /locus_tag="ACGI59_000747"
     CDS             41110..42033
                     /locus_tag="ACGI59_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019639192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9738553.1"
                     /translation="MNNLIIETKNLTKQYGAQKSVSNLNIHVQKGRIYGLLGRNGAGK
                     TTTMKMLLGLTKPTTGEIKIFGQDIHNNEKKLLPRIGCLIESPGFYPNLTGTENLKIF
                     ARLRGIPKKNAVESALDVVGLPNNDKKLFSQYSLGMKQRLAIALAIMHDPQLLILDEP
                     INGLDPIGIAEVRTFIRDLCTERGKTILISSHILSEIALLADDIGIIDHGILLEEESL
                     AQLEEKNSKYIHFAVSDAAQAARLLETQLNLKNFKVADNYNIMLYDTDVSVAEINRAF
                     ILNNIEVSESHLCEDTLEDYFKKVTGGEGIA"
     gene            42017..42787
                     /locus_tag="ACGI59_000748"
     CDS             42017..42787
                     /locus_tag="ACGI59_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9738554.1"
                     /translation="MKELLDIISCEFSKLKRLKFILISILGACLFPIPATILIAKDNL
                     PFEQLFKLVVNFGYFLLLPIVLSIVASQLFFIERDNDVLKNLATVPVQKGKLALAKLA
                     VLLFISLFYSVAGLGATIIGGFIVGIVEGVAVKLGMSIAFGIMFFVTVLPVVVLIVYF
                     NRSYIFSIILSFVYAIFNFSISLNIINFEPNNPILSVLPAPVIMRWWMAFWGDPTGEY
                     TALRQPYLLSTPACVGILCLITIIAVLLICMIYKKQEN"
     gene            42797..43579
                     /locus_tag="ACGI59_000749"
     CDS             42797..43579
                     /locus_tag="ACGI59_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9738555.1"
                     /translation="MLDIITTEFQKIKRYNILWIGVVAVLFSALLAVFQVVSSHGSDP
                     LTYESFANGVIWNNFSLSFPFGITLIGGYLINREYTDQTLKNILTVPISLRKLLVGKL
                     IAVGGITVLLSLFSFLCTFILGLLFCNINISVTLVVKSLVQILSVNLYCYVAVLPIIA
                     YFGRKQNAFLTGVGIAFVYGFCGVFVAGRNLTDFYPITAGLGLVGYNNGAGTVYQPLI
                     GAMTLIVILILTTILLVFTPNYDKVMTISKKKGIKHKKYSHK"
     gene            43956..44492
                     /gene="rbr"
                     /locus_tag="ACGI59_000750"
     CDS             43956..44492
                     /gene="rbr"
                     /locus_tag="ACGI59_000750"
                     /EC_number="1.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016692 - NADH peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin"
                     /protein_id="HFG9738556.1"
                     /translation="MNLKGTKTEKNLMAGFAGESEARNKYTYYASKARKEGYNQIAAI
                     FEETANNEKEHAKLWFKLLHDGMPSTEENLKDAAAGENYEWTDMYAKFAKEAREEGFD
                     KIAYLFEAVGKIEKEHEERYLKLLENLNEGKIFKRDEEVVWQCQNCGHVYVGTEAPEK
                     CPVCDHPKAYFNIKAENY"
     gene            44524..44913
                     /locus_tag="ACGI59_000751"
     CDS             44524..44913
                     /locus_tag="ACGI59_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HFG9738557.1"
                     /translation="MKFSKQRELILNEILNNPVHPTADYLYENLKKDNPNLSLGTVYR
                     NLAQLTEHGFIRKVSIPGYPDRFDGRIDNHYHIICEVCGEVYDLESEVLNNLQELISE
                     ETDIKITSYNISFKGICNNCKRCSQVG"
     gene            44950..45336
                     /locus_tag="ACGI59_000752"
     CDS             44950..45336
                     /locus_tag="ACGI59_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin family protein"
                     /protein_id="HFG9738558.1"
                     /translation="MCSEQKFFICETCGNLVGMIQSGGVPIFCCGKPMKELVPNTTDA
                     AVEKHVPVIEVDGNNVTVKVSSTTHPMTKEHHIAWVYLMTEQGGQRKCLAVDGEPVVK
                     FALNDDDKVISAYAYCNLHGLWKAEL"
     gene            45463..46905
                     /locus_tag="ACGI59_000753"
     CDS             45463..46905
                     /locus_tag="ACGI59_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HFG9738559.1"
                     /translation="MSIYKCSVCGYIYDESKNDKTWDELSEDWECPVCTKGRSYFGKI
                     STVYYEEDEKIAEDIVEDESKLNTEKEGDLNYLSTYLRRDDEVEKHMDIIHEMAVTGK
                     SIIEPMRTKLPVISWDDILIMGAQLNPLPLNEHDEVNTTTIIGKKAKKPMIIENPVYI
                     SHMSFGALSKELKIALAKGAAQNKTAMCSGEGGILPEEKEASYKYIFEYVPNKYSVTE
                     ENLKNSDAIEIKIGQGTKPGMGGHLPGEKVTEEIAKVRNKPVGQDVISPSCFEEIQSK
                     EDLKKLVDELREVSEGRPIGVKISAGHIEKDMEFIAYAKPDFVTIDGRGGATGASPKL
                     LKDATSIPTIFALYRARKYIDTHGLDIDLVITGGLRISTDFAKAIAMGADAVAIASSA
                     LMAAACQQYRICGSGKCPVGVATQDEELRKRLHIENSANRVANFLNVSLEELKTFARI
                     SGHKDIHDLSVDDLYTVNSEISNYTNIQHV"
     gene            47202..48017
                     /locus_tag="ACGI59_000754"
     CDS             47202..48017
                     /locus_tag="ACGI59_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFG9738560.1"
                     /translation="MERLKIQVLVDNNTYIDRYFVGEPAVSYYIEIDGNRILFDSGYS
                     DVFISNAEKLNIDLGNLTHVVFSHGHNDHTRGIQFLENRYDLSTVELISHPNCFIPRK
                     HGEKSIGAPFSAEEIKNIFMYNPKDKPFNLSKNCVFLGEIPSINDFEKRAKIGKCKIG
                     DLWEDDYVLDDSAIVCKTDKGIFIVTGCSHSGICNIVEYAKKVCGDDRVIGILGGFHL
                     FELNNRLDSTIQYLDKEEMRMLYPCHCVSFKVKAKMNEILNINEVGVGLTISI"
BASE COUNT        18016 a         5617 c         8869 g        15546 t
ORIGIN      
        1 ttattttatt tatttttgac aaatattata aaaattatgc caaagctaca tataaataaa
       61 taggagggga taattttgaa tagaatagta gatagaaaaa caaaaaatga atttagaaaa
      121 atcatagcta tttttttagt aattcttttc ttgtctgtat ttataggcag ttttatatat
      181 atagataaaa ctttaagacc gacaataaca gttttagcag agactaaagc tcttgaacta
      241 gcaaataggt cgataaataa agctgttgct gagatggtag aaggaaaaat aaattatgaa
      301 gatttaatgg atatacaact tgataataat ggtaaaataa ctatgataca agcaaacact
      361 ataatgatga atgaaattgc atctgcaata gctttagaaa tacaagatga attaaaaaag
      421 gataagacag caagttctta catacctata ggaacagcat taggaagtcc tatattagct
      481 aaatacggtc ctaaacttga agtatctatt gaacctatag gaacagtatc agtaaacttt
      541 aaaacggaat ttgagtcttc aggtataaat caaactagac atagaatata tttagaagct
      601 caaacacaag ttaaagtagt aataccactt ataacttcaa caaaacaaat taaagctcaa
      661 atacctattt gtgaaactat aatagtaggt gatgttccag aaagttatgt aaatattcca
      721 gaaaaaaatc taggaaatgt tttacctaac actggaaaga ataccaacaa gtaatgtaaa
      781 gttgtatatt tttaaataaa ttcaaaataa tagtttataa ataaaatttt tggaaataat
      841 attaatataa ttttaataaa aatttatatt ttatactatg taaaatatct aatatatttg
      901 gtctgtaggt taaataaaag tacctttaga ccaaatatat tattaataac ttaataacat
      961 cttaaaactt atttatttat attatttaca acatccattt aataaataca ccataaccta
     1021 cagctgggtc gttttcaatt gcatgtgaca ctgctccgat tattttatta tcttgaacta
     1081 ttggagtacc actcatacct tgtacaatac caccagtttg agctaaaagc tttttatctg
     1141 taattttaat tttaaaactt ttactttctg gatgtttttg gttttctata tctataattt
     1201 caatattata ttttttacac ttattaaagt tatcttgtaa aattatttga gcttttccta
     1261 atttaacttc atctaaagat gctactttat atttttttaa cttggaaata tcaaagttat
     1321 taatttgacc tcttattcca aaatcagtat tttctgtaaa gctaccaata atattttctc
     1381 gttttgcatt tatacatcca acactacctt tataagattt ttctatatta aggtgcttag
     1441 ttgtagaaat tgaacctgtt ttaataggta tttttcttgc attaccaaca cttattggat
     1501 gacctacagc tccaaactct gatgtatctg gattgatata cgttaaagta gcaaatccag
     1561 aaagtaaatt gttaaaacta attttttcta atatgtgttt ggtagttttc attgttataa
     1621 gttgtccacc tctatttaat aaaatagata cttgtttttc ttctggtaaa gcatttaagg
     1681 tattggcaaa gtcactatat ccatcaactt cagcattatt tacctttact atagcatcac
     1741 ccaatctaaa tggagatgct tctccaggat ttctaactat gatatttttt aattctgcgt
     1801 caatttgaag tacgtttcca attggtatta aatattcagg ttcatttttt tgtaaatttt
     1861 gtgcataaat taaattattt gagaaaaaat ataataaaaa taaaaatgtt aaaacaattg
     1921 taaattttga cttagctaag ttggtttttt tacttaaagt attattttta aaatgaaaat
     1981 ttttaagttg cataaaacac ctctttctta ttaaattatt ttaataatag aattgaagtt
     2041 ttcttgctgc ttctatatta tgttgtgcaa gaatcatgtt ttgatgcttg gaaaactagc
     2101 tcaataacac cttagttttc ataaaaataa ataaaataaa tagtgtatat gataaaataa
     2161 atacatattc atataaataa aaataaacaa taactgtcta gcataagaaa ggagcaaaaa
     2221 atgaaaaaat atagattgtt agttacttgt ataattgcca tgggaatcat aattatagga
     2281 acatcaaatc cagttagcaa tttttacaag actacaaatt tgattagttc aggaaatatc
     2341 acaaatgaaa aagcagataa agatgaaaat tcaaacaaaa ataataaaga taaaaatgaa
     2401 gacaaatcta attctaaaag taataaagat aagtcatcag aggacaaatc gtcagataat
     2461 aaaaatgaaa ttcaaaaaaa taaaaaactt ttaatttgta tagacccagg gcatcaagga
     2521 aaaggagata gcaatttaga accagtagct ccaggttctt cctctaaaaa agcgagagta
     2581 tcttcaggaa cagagggtat tgctacaaaa aagccagaat atgttttaaa cttagaagca
     2641 tctcttgttt taaagagtat attagaatct aaaggttata atgtaataat gactagagag
     2701 acacatgatg taaatataag taattcagaa agagcaatac ttgctaatga caaaaaagca
     2761 gatatggttg taagaataca tgcagatagt ttaaataatt cttcaaaaac tggagcatct
     2821 atattgattc ctgaaaaaga tggaaaatat acagctccta tatatgaaga aagtaataaa
     2881 tgtgctgaat ttatcaaaca gaatatggaa caatcaggta tacaaatcaa tggaatcgtt
     2941 caaagaggtg atttgactgg attcaactgg tcaaaagtac cagctgtatt agtagaaatg
     3001 ggatttatga gtaactataa tgaagaccaa atgatgtcaa atccagacta ccaaagaaaa
     3061 atgatgcagt gtatagctga tggattggat gcgtatttta aataagatat gaatagagaa
     3121 aaagttaggt tggtattatt tgcctaattt ttatctctat tttttattgt acataacaca
     3181 aagaagaagt ttattttagg ttaaattgtc gtaaatataa ctaaaaatat tgggaatact
     3241 tgatttaagt ttaaaaagaa tatattatag tatagaatgt gtaaaatttt tttgagaggt
     3301 gttgaaatgc tagtagtaga aaatgtgagt catggatttg gtgcgagaac aatattagaa
     3361 aatgtatcct ttagacttag aaaaggtgaa catattgcac tagttggtgc taatggagaa
     3421 ggaaaatcga gttttttaaa tataataaca aagaagctta tgccagatgc tggaaatata
     3481 aaatggtctt caagagctac agtaggttac ttagaccaac atacggtatt aagcaaggga
     3541 aaaactataa gagaagtgct tagagaggct tttaagcata tgtttgactt agagcaagaa
     3601 atgattgcta tgtatgacaa aatgggagaa gctagtgatg atgaaatgag taagcttctt
     3661 gaagaaacag cagagataca aactatactt gaaaatagtg gtttttatat gatagatgca
     3721 aagatacaag aagttgcaaa tggtcttggt cttggagaaa ttggtcttga taaagatgta
     3781 actgacttaa gtggagggca aagaacaaaa gtacttctta ctaaacttct acttgaaaat
     3841 ccaactattc ttatattgga tgagcctaca aactatttag acgaagagca tataacatgg
     3901 cttactaagt accttcaaga gtatgagaat agttttgtct tagtttctca tgatattgaa
     3961 tttataaata atacatgtaa tgtaatatat catatggaaa atggagagtt aaatagatat
     4021 aaaggaaatt atgatgaatt tgtaagactt aatgatataa agaaacgtca agaagaacaa
     4081 gcttatgata aacaagttga agaaagaaaa agactagagg attttgtagc tagaaataaa
     4141 gctagagtag caactagagg aatggctaac agtaggcaaa agcaacttga taagatggaa
     4201 atattagaaa gaccaaaaga aaaaataaaa cctacttttg cttttaaaga tgctagagca
     4261 gctagtaaaa taatttttga aacagaaaat ttagttttag ggtatgatga agcattgaca
     4321 aaaccactta atttccattt agaaagagga aaaaagattg ccttaaaagg aatgaatggt
     4381 attggaaaat ctaccctatt aaaaacttta cttggaataa taaaaccatt tgaaggaaat
     4441 gttaaattag gtgactattt ggaagttgga tattttgaac aagagagttc aagagaaaat
     4501 agtaatacac ctatggatga agtttggagt gaatttccag ggcttacaaa ctttgaagtt
     4561 agacaggctc ttgcaaaatg tggacttaca aatgaacata taacgagtca aatgagagta
     4621 ttaagtggtg gagaagctgc taaggttaga ctttgcaaag taatgttaaa aaatataaat
     4681 ttcttagtat tagatgagcc tacaaatcat ttagatgttg aggcaaaaga tgaattgaag
     4741 aaggctatca aagagtttaa gggaacagtt cttttagtat gtcatgagcc agaattttat
     4801 tcagaaattg ttgatgatgt atggaatata gaagatttta caactaaaat cgtgtagtga
     4861 tttaacttat tgctgggggg atttcatgaa aaagtttcta aatgtacttt tttcaatttg
     4921 tattagtatt gttataatag taggtgttat taattttact gttggattta aacaacttta
     4981 ttattttgac attgattact taaatatatc agagttatct ggtttatcta aagatgatat
     5041 aaaattgaac tacgattatc taattgatta taacttaaat aagaatgtgt ctgagttcaa
     5101 attaccaaca ttaaaatctt ctccacaagg gaagatacat tttgaagaag tcaggaatat
     5161 atttcaaaat ataaataaat tagctaaatt attattggta gtttctttag taggaattat
     5221 attgagtgta aaaaataaaa atataaaaat cttaaaaact acatcaataa cacttataat
     5281 tatgccatta ttattaacag ttccaatttt attaaatttt gaaaagtcat ttattatttt
     5341 tcataaatta ttatttagaa atgattattg gatatttaat cccgatttag acccagttat
     5401 aaatatattg ccagaggaat ttttttttca ctctggaatg atgattttaa tattaatttt
     5461 gttagtaagt atagtactgt ttgtaatgta taaattatgt aaatctttat caataaaaat
     5521 atttcaatgt taaatagagg gatattttta tttccaaaat aagataataa aaaaggatta
     5581 gattaaaagt tatacaataa aaatagctgt gcaataatat ttagactatg aaagtagttg
     5641 aaaaatattt atgcacagct atttataata gaatttactt tttactagtg attttaacta
     5701 aatcttgaaa tttactcata atgtaattgt aattatcttt tttatgaggt aatatacaac
     5761 tactgcaatc tttgatacct ttatctgtat acctaaagtt tccaccacag ttttctccta
     5821 aagcatacaa aggacaatag caaaatagac agttaaactc ttctggttta ttagttttat
     5881 gacaagggaa aaattcgcaa tctttgtgat taaaaaactt ataattttcg cccatatttt
     5941 cctcctaatt aaataaaatt taaatttttt atactatata agattttggt gataatataa
     6001 ttatactaaa taattttatg aatagtcgat aatatagtat tattaatcat agtattaatg
     6061 gtataattag tcaacagaaa aataatgtaa aaataatgta agtgtatttt gtaattagaa
     6121 tatgttttat aacaaaaaat gaggtgatta agtgaaagaa attcaagaaa aagcacttct
     6181 tgtaggtctg aatttgacta caatagtgaa aaaaaatgat gatatagaca ctaatgaatc
     6241 tatggaagaa ttaaaagaac taacaaaagc tgctggggca gaagttgtag gtagcttaat
     6301 tcaaaataaa cactctgtag atgcagctta ttatatagga aaaggtaaag tagaagaaat
     6361 aagagcctat agtgactctt tagatgctac tttagtaatt tttaatgatg aattatctgg
     6421 tgctcaaatc agaaatatag agaatgttgt tggaagaaaa gtaatagata gaactacttt
     6481 aattttagat atatttgctc aaagggcact tagcaaagaa ggtaaattac aagttgaatt
     6541 agcacaatta aaatatagat tgccacgact ttatggaatg ggtggtgaga tgagtagaac
     6601 aggtgcaggg attggtacta gaggtcctgg agaacaaaaa ctagaaatag ataagagaca
     6661 tatacttaat aaagcagcag atataagacg tgaacttaaa gaagtaaaaa agaatagaga
     6721 gacacagaga gttaagcgtt taaagtcaaa tataccgatt gtagcacttg ttgggtatac
     6781 aaatgcagga aaatctaccc tgttgaacga attaataaag acacacaagg attatgaaca
     6841 agaaaaagaa gtttttgtaa aagatatgct ttttgcaact ttagatgtaa ctttgagaaa
     6901 ggctcttttg ccgaataaaa aagaattttt agtagtagat actgttggat ttgtaagtaa
     6961 acttccccat gatttagtag aagcgtttaa ggcaacattg gaagaagtac agtatgcaaa
     7021 tttaatctta catgtaattg atgcaacaaa tactagttat gaattgcaaa aaagtacgac
     7081 tgaaggtgta ttaaaagaac ttggtgtaaa tgataaaaaa catatacttg tatataataa
     7141 agtggacaag ctagaattgg atatataccc taaaagtcaa gaagatattg tgtatatttc
     7201 tgcaaaacaa ggcataaata tggataagtt attaaatatg atagaaattg ctttaatgga
     7261 aaatacttat tcagtaagtt tgatgttacc ttatgagaga ggtgatatat ttagtagaat
     7321 aaaagataaa tataatgtgg agaattttga gtatggggaa agtggtataa cgctagatgc
     7381 agatttagat gaagaagatt ttaatatcta tagagagtac atactagaaa aataggttga
     7441 atatggatat aaactgggaa aatatagata atcttgaaga ttattttata acctatcttc
     7501 tttataaaga atctaaaaca gtatctcaaa ttagtaaaat tagaaatatt tcaagtactg
     7561 aagcaagtga gcaacttata caagcaaagt taaaaataaa ggaaatgcaa aaagatgatt
     7621 ttgaggcatc taaggatatt ttggataaat ttctagaact agataaaatt aagagacttg
     7681 attttatgga tagcttagat gacgaaaaaa tggtatactt taagagaaag gttttcaaaa
     7741 gaatccttgt agaaaaaaat gctgaagatt taattgtttt aatttgggcg acaggtgagc
     7801 taaaagatga taggtttttg aaattgcttc atcagctcac aaaccacaga cactcagata
     7861 ttagaagaat aacttattca gcaatcagaa agattgaatc accaagtagt agagaagtac
     7921 tgcaaaaagg tctctacgac aaaaatgcac agactaggca gtactgtgca aaagctcttt
     7981 caaaattggg agatgaaaac agtttaaaga ttttacagca gttgaaagac aagaataaga
     8041 attttgaaaa agaatatgtg cttagagctt atgacgaagc cattaagtca ttggaaaatg
     8101 caaaaaattt gaaaaagtaa ttttttatta aagcaatttg tttacttaat gatgtatttc
     8161 taaagtttaa caaggtaata aataaattaa tgggaggggt ttcaaatgat agtaagacgt
     8221 tgtgcaggtg gagtagtgtt ttatgctaat aaagtactta ttgtaaaaaa tgataggggt
     8281 gaatggacac ttccaaaagg aaagatacta ggagggggac ttccatatga gagtgctgtt
     8341 caaagagtta aagtagaaac tggcatagat gctaaaatga tagatgtggc tggcgatact
     8401 atgtatgaat ttttctcaag aagtagacaa caagaagttt gtaatgcaat aatgtggtat
     8461 gttatggaag cttgtaacac tgattgtgta cttgctcctg aatttcaaga aggtggattt
     8521 tataaagtaa aggatgcttt agaaatgtta tcacatcata aagagcaagc tttagtagaa
     8581 gtatcttata aaaagtttaa ggaattaaag aaattagttt cagagggcga tgatgttcca
     8641 gcaaattaat tgtgaattta gaggattctg aggaagggat agccccttcc ttttttaatt
     8701 gaagcagttt taagcgtaaa ttatatagtc tctttaaact atgatttatc aatttagaga
     8761 tttagtagtt tatatttaaa atcagtaaga tatatttaaa ataaaaatta aaaagtatac
     8821 aattttatta tataattgtc ttatgatgtt gattaaagaa gtattaaaag tccatttatt
     8881 tttaaataga atgattttat aaatatatat ttgatgtgta tattagaaaa tgattaaaat
     8941 aggaggaaat acaatgagca attacaggac attacatgaa tttggaacgg atgaaataac
     9001 gattgaaaag tctgttttta ttggatatgc taagcctata aagagtgaag aagaagcatt
     9061 agagtttata aatgaaataa agaaaaagca taaagatgca actcataatg tgtgggctta
     9121 tactgtaggt aaaaatatga atatacaaag atatagtgat gatggagaac cacaaggaac
     9181 agctgggata cctacattag aggttataaa aaaagaagat ttgagagatg tagcggttgt
     9241 tgttactagg tattttggag gaacaaaatt aggtgcaggt ggtctagtta gagcttatac
     9301 aaaaggtgct aaactgggac ttgaagcagg taagataatt tataaggtaa tgtatcaaga
     9361 agttaaggtt aagatagatt atactcaact tggaaaagtg caaaatgagt taatgaattt
     9421 ggggtatttt ataaaagata ctgtatatga ggataatgtt gaaatagtag tatattcaag
     9481 attagaagat gtagagaaat tatcagaaaa aatgattgat ataacaagtg gaacaggaaa
     9541 aatagtttta ggagaggaat tttatttgtc tgaacaaaat ggagaaattt taatataatc
     9601 taaaaggtta ttagaatagt taatacctca aatagccaat tagtattttt aagtgaaaag
     9661 ccattaaatt gatttaatct aaatcaattt aatggctttt tatcggattt tgcacagaat
     9721 ccttttttta cttcaaataa accgactaaa agtaaaaata tcccaaatat tttttttaat
     9781 atatctgaag acagataatt agctaatagt gaaccaaata cagctcctaa aaccccaaac
     9841 acagctatag gtataaccaa tttaaaaatt acatttttat tttttatatg tatagcaagt
     9901 gctataagtg tcatagggat agatgaaagt aaatttacgc tttgtgctat tttagtgtct
     9961 atgccagcaa gtaaaacgag tgcaggtata aggattgttc ctccacccat tcccattcct
    10021 ccaataattc ctgcaaaaaa tccaattaga gcaaataaca ttaaaacacc atccttaatg
    10081 ctgcaattat cataattatt ccaaaagaaa ttctaagaaa ctttccagta accttattta
    10141 gtaatttagc tcctataata ccaccaagaa tacttccaac tgcaacttta attgttaagt
    10201 taacatcata agtaccttta gatacatata aaacagaact tgctgtcgtt aagaatatta
    10261 ttatagaaag agcagttgca tgagatttat gttcttctac cttaacgatg tcatttaata
    10321 ttggaactaa aagagtacct cctccagaac caaaaactcc atttataaat cctgtaaaaa
    10381 ctccaattat cgtatttttt aaattaaaat caaagtattt tttctttaaa tcatcagttt
    10441 ttttcataaa aatcaccttc tgaaatagtt ttaccaaaat aggcaaatta tatttatata
    10501 aaataagatt aaatataatc tttcatgttt gtttaatttt gcataaaaaa aaacatatta
    10561 aaacaacata ttaatatatg gtataatata ttaaaacaaa gtagcaaatt tgagaggaga
    10621 taacaatgtc aaatataaaa atacaaatag acaaaactgt agaatggctt ataaataagg
    10681 ttaatgaggc aaatgcaaaa ggattaattg ttggtgtatc tggaggaatt gattcggcag
    10741 tagttgctaa tttaattaaa aaggcatttc cagaaaactc tatgggagtt ataatgagca
    10801 taaaaagtaa tcctcaagat agagaagatg cacttaaggt tatagaggga tgtgatatag
    10861 aataccttga tttagacctt atagaaccac aaagtgctat attagacatg gtagtaggaa
    10921 atttaaaaga taaacattta tatagagaag aatatttaaa gatgacagat gcaaacttaa
    10981 gagcaagagt aagaatgagt acaatttata caattgctaa caatctagga tatctggttg
    11041 ttggtacaga caatgctgca gaaatacaca caggatattt tactaaattt ggtgatggtg
    11101 gagttgatat tttacctatt gcaaatttaa ctaaaggaga ggtgtatgaa tgggcaaaag
    11161 agcttggtgt tcatgaagat ttaataaaca aagctccttc agcaggtctt tgggaaggtc
    11221 aaacagatga agatgaaatg ggaactactt acaatatgat agatgctgta ttagaaggta
    11281 gattggatga agttcctaaa agagaccaag agataattga aagacttcat agattaagtg
    11341 aacacaagag aaaaacgcct gcacaacctc ctaagttcta atttactatt tataagattt
    11401 gtgataaaat ttacaagtga tgaaaattag aataattaat tgtacgtatt aaggagtgga
    11461 aatatgggac gtataggtaa tataattaat agaaaaggta aacaagatgc tcaaagagca
    11521 aagatattta ctaaacatgc tagagcaata gcagttgcag ctaaggaagg tggagcagac
    11581 ccagagtaca atgcagcgtt aaaaacagct atagaaaaag ctaaggcaga taatatgcca
    11641 aatgataata tagatagagc tatcgctaaa ggtgctggag caggtgctgg agaagactat
    11701 gaaactatag tttatgaagg atatggacct ggtggagttg ctgttatagt agaaacttta
    11761 actgacaaca aaaacagaac tgctggtaac gtaagatact attttgacaa aaacggagga
    11821 aacttaggaa ctagtggatg tgtgtctttc atgtttgata aaaaaggaca aatattagtt
    11881 ggtttaggag atggagtttc agaagaagaa ttgatggatg ttgcattaga agcaggagct
    11941 gaagatttca taacagaaga agatggttat gaaataataa ctacaccaga agatttttct
    12001 agtgttcgtg atgagttaaa agctaaagga tatgagttta tatctgctga tgtgaagatg
    12061 atacctcaaa ctacaactgt attgactgaa gaatctcatt taaaaatgat gaataaatta
    12121 gttgatatgc ttgaagaaga tgatgatgtt caagatatat atcataactg ggaagtagaa
    12181 taaatagtat aagcatattg aaatttataa gtattgaatc aattttaaca tgtgataaat
    12241 tttatagaat aagaaacact aaaatagaca tacgcacctc taacctatat ttgcataata
    12301 atatgtaagc ataggtaagg ggtgttttta tatttatagg ttaagaaatt taaagtaaat
    12361 tttgagctgg actcgattgt ctataaataa ttgatgggta tataacttgt tgctgaatac
    12421 catttatacg cccagttact gtatcaataa tcatcctaag actacgttct gctgtctctt
    12481 ccatttttaa atctataact gtcaaaggag gaatacttct tgccattagt cgtgaaggtc
    12541 cattacttgc tgcaaatatt tgaatatcct ctccaattac aattcccgat tcatgaaatg
    12601 cattcatcat accaagagct accacatcat gattacaaaa aattactttt tttagtgaat
    12661 tttttgcaag cattttttga gcaatatcat atccactatc tattttatta gaacaataat
    12721 atatagaatc tgatagatgt attccatgtt ctttacatgt ttttaaaaaa gatttacccc
    12781 ttaaagtcat acatgtaaag gaagttgggt tgaatacaac agcaatatca tttttaccat
    12841 tttgaatagc atgtaaagct gctatacgac cagtttcctc attatcaata gatacacttg
    12901 aaaaaccagg tatttcacga tttacaacaa ctacaggaat tttaggttta ttttttacta
    12961 gatattctat atcatcagca ttgatacctg ttaaaacaat tccatcataa taattatttg
    13021 caaaaaagta ttcatcatca catagatgac caactttgta aggtcgtatg atgatattta
    13081 tgtttaaatg ttctaaagaa attatgtggt ttattgcttg aagtatattg gttgatgcca
    13141 actcaaatcc ctcatcctgc caataaaaag ctattttaat attattatgt ttaataggag
    13201 tagagcgttt atatccaagc ttatcagctg aatttaatat aagctcttgt gtatgtagcg
    13261 gaatcctgcg ttcatttcct ttaccactta agacgtacga gacagttgct gaagaaagac
    13321 cacagtcttt tgcaatatct ttgattgttg ccatgataaa atcctcctta aatagttgct
    13381 taaatttgct tataccaata atcttaacat atattcaact aaagaacaat attctacaag
    13441 gtgcatattt tacaggatta gaataaataa attaaaaaaa acttcttact tatgttgaca
    13501 taatttagtt tataggatta taatatgctt aaataagctt atgattaact tgaattaagc
    13561 aaagttaagc aggaacatgc aatgctagct tgcaatgtga ctgcaaaggg ggaaaaggta
    13621 tgagtaaggt aaatatattt gaagttggtc ctagagatgg atttcaaaac ctgaaagagt
    13681 ttttagaaac tgataaaaag cttaagatta ttgatggtct tattgcttct gggattaaac
    13741 aacttgaaat tagttcattt gtaagcccaa aagcaatacc tcagatgaaa gatgcaaaag
    13801 aagtagcaac ttattgtgta gaaaaatatc ctgatgtgaa actttatgca ttagttccaa
    13861 atttatatgg tgctaaagtt gcatgggagt gtggtataag agatatatcc tatgttatat
    13921 ctttgagtga aactcataat aaaaaaaata taaatcgtac ccatgaagag tctattgatg
    13981 agcttaaaaa aataatgaat acttatccag acatgaatat atgtattgga atggctactg
    14041 tatttggatg tccttttgaa ggtttaccaa aaattgaaga tgccataaga tttgctaaac
    14101 gcctttggga aaacggtatc agggagataa atctagcaga tacaataggg attgctgacc
    14161 ctaagcaagt aagagaaact attagtgcat tagttgaagc tttaccagaa tgcagatggc
    14221 aagtacatat tcatgatact agaaatatgg gaatggttaa tactcttgtg gctattgaat
    14281 ctggaattac tactgttcag tctacacttg gaggattagg aggatgccca tttgctcctg
    14341 gagcatctgg caatactgca actgaagatt tagtatatat gcttgaacgt atggggtatg
    14401 atacaggagt tgattttgtt aaacttcttg aattatcaaa atatcagaag agttttgtaa
    14461 atggagtata tagtggtcat catgtaaata taaagtctga tagttgtaat tgttagttct
    14521 attgattaat cgattaaaaa aatatgctga ttggtaaaat agatttattg ataaaaaata
    14581 taaattataa aatacattat ctaataaaac aaataaaaaa attgaggggg attagtatgg
    14641 atattatagc tttactttta tttttaggag ttattattat tgcatttgta cgtaagaata
    14701 atgcaggtat tttggctcta gctattggtg tcatagctgt tagaattttt ggaatggatg
    14761 ataaagaact ttatggagct ataagcaact caatgtttac tacacttgtt ggtataacac
    14821 tactctttgc aatagtgaac tcaacaggag cattaaagct tgttgcagaa aaaattgtag
    14881 ctgcttctgg taaacgtgtt tggcttatac ctatagcagt ttttatagca ggatttgttg
    14941 ttgctggtgt tggacctggg gcaattccag cacttgcaat tatcccagca atggctgttc
    15001 ctgtagccat aacagttggt tataatcctg taatgcttgc acttataggt gaatgtggat
    15061 taatggcagg tcgtatgact cctattactc cagaaggtca aattataaca accgttgcag
    15121 aaggggttgg aattgaaaat gttatgccta ctatcttagc ctgtcaaact ctttctacaa
    15181 ttgtattttc aatagtattg tttataattt tcaaaggata taaacttaaa aaacctataa
    15241 atgttataga tagtaagcaa atagaaaaat tcaatataaa gcagataatt tctctaataa
    15301 gcatagtagt tatgattgct tttatcataa tttttgatat gaatgttggg cttgcagctt
    15361 ttgcagtttc agctgtactt atttttttca atattgctga tgaaggagaa tgtatcaaat
    15421 caattccatg gagcacgata gtaatggttc ttggagtagg tgcaatgatg acaatcgtag
    15481 atgcagcagg aggtattgac ttgatgagta atgcattatc atcactgatg aatagtaaaa
    15541 cagctactcc aattatgagt atttcagcag gacttatgtc tatggttagt tctgctttag
    15601 cagtagttta tccaactatg atgcctatgt gtgttgacat agctaaggca gttggaggag
    15661 tagacccact agcacttatt gctgcagttg gtgttggagg ttctcttgct ggtgtaagcc
    15721 cactttctac tggtggggca ttgattttag cagctatggg ttcaagtaag aaagacttta
    15781 ataaggaaga acaaaataag gtttttgtac agcttttcat tatgagtgca gttggcttat
    15841 tggttattgc agtagtatca atattatcat ttaatgcaat aacacattta atgaactaat
    15901 tagaataact gaaaataaat cattaaattt aacttttaat attaatcaat ataatataat
    15961 ttggagggga agttatgaaa aaaggagcat taagcaatat aaaggtactt gaccttacaa
    16021 gagttcttgc aggtccatat tgtacaatga tgttggcgga ttttggagct gatgttataa
    16081 aaatagagat gccaagaaaa ggtgatgata caagaggttt tggacctttt gtaaatggag
    16141 agagtttata ctatgcaaat ataaatagaa ataaaaaagg tatcactcta aaccttaaat
    16201 cagaagaagg aaaaaatata tttttagaaa tggtaaaaca tgctgatgtt gtagtagaaa
    16261 attacagacc aggtgtaatg gaaaagttag gtctgggtta tgatgagttg gctaaagtta
    16321 acaatcaaat aatttatgga gctgtatcag gatttggatg ttatggacca tattcagaac
    16381 gtccaggtta tgatattatt gcacaggcaa tgggtggtct tatgagtata acagggcaag
    16441 aaggaggaga accaacgaga gttggtaatg ctatgggtga tgtactaggt ggaatgaatt
    16501 taactattgg tatactcaca gcactcaacg ctagaaaaat aataggaaaa ggacaaagag
    16561 tagatgtttc cctcgttgat tctgttgtat cgtctctcga aactggtata cagagatact
    16621 ttgattcagg acaacctgaa cttatgggta atagatatgc ttcagcctat ccttatgact
    16681 cttttaaggc aagtgatgga agttttgtaa taggctgtgg taatgacaaa ctttttgaaa
    16741 agttatgtaa aaaagttctt tttagagaag accttttaat agatgagaga tttaaaacaa
    16801 atcttaatcg ttgtgaacat tacaaggctc ttaaaccaga aatagaaaaa tggtctgtta
    16861 agcatacaat aaaagaagca gttgacttaa tatcagaggc tggagttcct gctgctccta
    16921 tctataatct tgattgtgtt acaacagatg aacatatagc aaaagcaaga gaaatgattg
    16981 tagatattag tcatcctatt ataggaaata tgaaagtcaa tggaaatcca gttaagttga
    17041 tgacaacaaa agctaaaatt gatatgccag caccatctct aggggaaagt aacgaggaga
    17101 tttataatgg ttggcttggt tttgatagtg ataaaataaa agatttaaaa gaaaatggag
    17161 ttatttgatt aaaaaatagc taaacacact tgtgacttta gtcatgggag gttcagttag
    17221 aataatatca aaaaatatta aatcaatact gtaaataaaa tatagaattg tcagaagcat
    17281 ttaagatggg aaaagctact tagacattga gtctttgtgg cttttcctat tttcattaag
    17341 aattttatat tatcagaata gatatgtact aaactaaagt attaacaatt taaattaata
    17401 gaaaataaac tacctaatta tgttgaataa agactacttt agtgaaaata aacttataga
    17461 gatgctattt aaattatttt aatggaaata tcaaaaatga gactgcattc tcaaaaatga
    17521 gactgcgttt tttaagtatt ttctcgaaaa tgagacaaaa atattgtttt atgggtttta
    17581 ttgaagaaaa attcttctgt gaattacaat ttaatcaatt ttaaagttga aaattattaa
    17641 aaatttagtg aaatttatgt atatttcgtt tattttatta aaaataacaa ttttttacta
    17701 aaaataaaga acttcaatgg tgattggcat agtacttgct attttatatt aacaagaaat
    17761 gtagaaaaaa taacaatgta taatgggggg aatatgatgg aatctgtaat ggaaaatttg
    17821 aacaatctta aggtggaggt taaggacaaa gtctgtgtta ttactataaa tagacctaaa
    17881 gctttaaatg ctcttaatag tgacactctt agggaactat cacaagttat tgatgtagta
    17941 tcagaaaatg aggcaatatt aggagttatt attactggag aaggtaaagt ttttgtagcc
    18001 ggtgctgata tacgccagat gcaaaactat aaaagtgagg aaggcagaaa atatgcaggt
    18061 tatgctcagg gaatttttga taaaattgaa gctcttgaga aaacagtaat agcagcagtt
    18121 aatggatatg cattaggtgg tggatgtgaa cttgctatga gttgtgatat tagaattgca
    18181 tcagaaaagg ctatatttgg acaacctgaa gttaatttag gagttattcc atgttttggt
    18241 ggaactcaaa gattatcaag acttgttgga actggaatag ctaaagagtt aatttttacg
    18301 gggagacaag taaatgcaga agaagctaaa tctattggac ttattaataa agtggttcca
    18361 agtgatttac ttttagaaga gtctatgaag atgatgaacc aaatagttga aaaagcgcca
    18421 atagcaataa gatatgcaaa agttgttatt aacaaaggga tagatatgga tttaaagaat
    18481 gctcttgaac tagagaaaga tgttgcaggt ttaacttttg ctacaagaga taagcaagaa
    18541 ggaatgaatg catttataga aaagagaaag ccagtttttg aaaataaata gctattataa
    18601 ctaaaattgg aacaatgtag aatatattca aaatctaaat ttcgtgaaaa aatacatata
    18661 gtttaatttt aaaaggagtt aatttatgaa tacgggagta attataagtt tagtagggat
    18721 tatcatagct ctatctcttc tagttgtaat ggttatgaag ggaataaata tttttattat
    18781 agcaattgta tgttcatcat tagttgctat aacaggaagc ctaaatgtgt atgatgcact
    18841 taaagtggat tatatgacag ggtttgttgg atttcttcag gcaaatttct ttattttcct
    18901 aacagggaca ttaatgggaa aaataatgga gattactggt ggagcaaaat ctattgctaa
    18961 gatgattgta agatggattg ggaaggataa agcactactt tctattccaa tagcttgtgg
    19021 aatattagcc tatggtggag taagtgtttt cgttgtaagt tttgcagtgt ttccgatagc
    19081 tttagaaatt tttaaagaag ctgatttgcc acgaagattt ataccagcag ctttgacttt
    19141 tggatgctca acttttgcta tggttgcacc aggggcacca caaattcaga atattgttcc
    19201 agcatcaaca ttagggacag atattatggc tggagcagta aatggtttta tatcatgtgc
    19261 agtaatgttt atagttggaa gtacaatttt gtacaaaatg gtttcaaaag aaaaagccaa
    19321 tggtggtcat tttatagcac atgaatcaga tatatttgat gaagcagaag caactaattc
    19381 taagagagaa aatggtccca atggtgtaat tgccttaatt ccattgatag tgtcgatttt
    19441 aataattaat gtaaaagtta atggaaaagc gatagtacca attgaggtgg gagtatttat
    19501 tggagcttta ttagtatatt tattgttaaa taaatatcaa gataatacta aaatagttgg
    19561 acatgttggt gatgcatgta aaactacagt agtagcaata tgtaatactt gtgctgtagt
    19621 tgcatttggt tcagtagtaa aatcagcagt tggttttgac ttcatagtta atgctatgac
    19681 taatattcct ggtcctccta ttgcaggggc tgcagttgga actacagtta ttgcaggtat
    19741 ttgtgggtca gcttcaggtg gactaggaat tgcagctcca cttcttggac cagtattttt
    19801 agccaagggt gtttccgctg cagcattaac taggactatg gcaatttcat cagcagcatt
    19861 agattcactt ccacacaatg gatatattgt tactgtaaca aatggtcttt gtaaagaaac
    19921 acataaggat gcctatatgc caatattctg ggtaactgtt ttaaccccat ttattggaac
    19981 catagtagca gtcctactgt ttacaatgtt cccgggattg ccataggaga aaagataaaa
    20041 ggtataaatt atattttgat attataaaat caattttcaa aatatataca ataaatttca
    20101 tgttttaata ctgtaaatat gtatttaaaa taacaaattg accatataag gaggaattga
    20161 aagtgttaaa taaaccatta gagggagtta aggttattga cctaacgtat tttgtagcag
    20221 gtccagggac ttctaaaata ttagctgact ggggtgctga cgttattaag gtagagccaa
    20281 gctttggaga cccaggaaga aagacagggg caacaatgac aatgccaata gatgattata
    20341 ataatccttt ttacagcaca tataattcaa ataaaagagg tttgtctata aatcttaaga
    20401 gtgaaactgg aattgaaatt atggataagc ttttaagcga agcaaacgta tttgtttcaa
    20461 gttatagaac aggagcatta aaaagactag gtcttgatta tgaatcatta agtaaaaagc
    20521 atccacacct tatctggggt cagataaatg gttttggcga ttttggacct gctaaggata
    20581 atgcaggatt tgatacagtt gccttttggg caagaagtgg ggctatgctt gatatagcag
    20641 aaaaagatac aagtccaata aatccaccta tagcctttgg agatgcaaca acttcttgta
    20701 gtttatctgg aggaatatgt gcagctttgt accagcaagc taagactgga aaaggacaaa
    20761 aagtaatggc atctttattt gggcaagcaa tatggaatgc atcatcttta ttagcatcta
    20821 cacagtttag tgatgagtat ccaaaaacta gaaaaaatgc tatttcacca gtaataaatt
    20881 cttatcgttg taaggatgga aagtggatat tcttaagtat tttagagcat gaaagatatt
    20941 ttgaggcact ttgtagatta tttggaagag aagacttaat aaaagaagag aaatttgcat
    21001 cttcagtaga aagtaagaaa aatgctacag aacttattaa tattttagat ggtgaatttg
    21061 caaagtttac tcaaaatgag atggttgaaa aacttactgc tgctgatata gctcatgaaa
    21121 aaattcaaca tgttaatgat gtagtgactg atgaacaggc tattgctaat aactatatat
    21181 atgaacatac aagtaaagat ggaaataaga caatgcttgc atctactcca gttaaatttg
    21241 gaaatattga agttaacatg acttgtgatg ctccacttat aggagaacat tcagatgaga
    21301 tattgagaga acttggatac agtgattctg atatagagac tctaattgaa tctggaattg
    21361 taacaatgaa taaagaagca tgtgaaatat gttaataaac atgtgaagta tgttaataaa
    21421 taggagggaa ttttatggat tttagattaa cagaagcaca attaatgctt caaagagtag
    21481 caaaagaatt tgcagagaat gaaatagcac caatagctgc tgaaacagat aaaacaggta
    21541 tttttccaag ggagcttttt agcaaaatgg ctaaaatagg atttaatgga attggaacac
    21601 ctgtagagta tagtggttct ggtggggcag atatagaaaa ggtaattgtt gttactgaga
    21661 ttgccaaaaa atgtgcagca tctgcagcaa tattatcaat acacacaata tatgctcaag
    21721 ctattttgaa atttggtact gaggaacaaa agaaaaaata ccttccaatg atggctgaag
    21781 gtgggtgtgt aggtgctttt gccttaaccg aacctaatgc tggttcagat gctgcgagag
    21841 ctgcaacaac tgcaattatt gatgaagaaa ctgatgaata tgttttaaat ggaactaagt
    21901 gttttatttc tggtggaggt caagcagaat cattaataat atttgcattg acagacccat
    21961 caaagggaat aaagggaatg tctgctatca ttgtcgacaa aggtactcca ggtttttcaa
    22021 taggaaaaat tgaagaaaaa atgggtattc atggttcaga aactgcagag ttaatatttg
    22081 ataactgtag agttccaaag tctaacttat taggaaaaga aggtaaagga tttaatatag
    22141 ctatgacttg tttagatgga gcaagaattg gagttggagc tcaagctgta ggaattgctg
    22201 aaggggcttt agaagaaagt atcaaatatt ctaaagaaag agtccagttt ggcaagccaa
    22261 tttcagcttt gcaaggtatt caatggtata tagcagatat ggcgacaatg gtggaatcag
    22321 caaaattatt agtatattat gctgcagatt tgaaagctag aggagaaaaa cacactaaag
    22381 aagctgctat ggcaaaatac aatgcgtcac gtactgcaag agaggttaca aatttagccc
    22441 ttcaaatcca tggtggatat ggatatatga aggattatcc attagagaga atgtacagag
    22501 atgccaaaat aacagaaatt tacgaaggta cttcagaaat acacaaagtt gttatttcaa
    22561 gagcagtact aggataggag gaaaagaatg aatatagttg tttgcttaaa gcaagttcca
    22621 gatactaatg aagttaaaat aaataaagag acaggtactc taataagaga tggtgtacca
    22681 agtataatca atccagatga tagaaatgct ttagaagaag ctctaaaaat gaaagatgaa
    22741 ctaggagcag tgattaaggt tataagcatg ggacctcctc aagctaaaag tgctttaaaa
    22801 gaagctttgg caatgggagc tgatgaagct tatttaatct cagatagagc ttttggtggt
    22861 tcagatactt gggctacatc tacaattatt gctgctgcta tagagaaggt tggaaagtat
    22921 gatgtaatat tctgcggaag acaagctata gatggagata cagctcaggt tggaccagag
    22981 gttgcagaat ttttagggat accacaagtt acttatgcga aagaagtaaa ggttcaagat
    23041 gacaaattac tagtaactcg ttatacagag acaggggatt atttaataga agcaaagatg
    23101 ccagttttat taactgctat aaaagagtta aacaatccaa gatatccaag tgtcaaggga
    23161 atcttagaag cttataataa tggagatgca aagattactg tattaacact tgcagattta
    23221 gatgttgata caactcaaat tggattaaag ggttcaccta caaacgttta taagtctttt
    23281 gtaccagtta aagacaagca taatgaaata atagaaggta taaataaaaa agaaaaagct
    23341 gagaaactaa tagaaatact attcgatttg aaactagtat aggagtgtta agatgatgag
    23401 agcaaaagtt aatcaaggta taaatttaaa tgattataat ggtgtttggg taataggaga
    23461 gcaaagggaa ggtaaaatta atcctgttac gatagaactt attggtgaag gaagaaagct
    23521 tgctgaccaa ttaggaaaag agcttgcagt tgtgatagct ggatatgaag tagagaaaga
    23581 agtgaaggaa ttacttcact atagtgttga taaagtatat tacatcaatg accctcttct
    23641 taaagatttc actactgatg gatatgcaat ttcaattgca aatttaatcg aaagaaaaaa
    23701 gccagaggtt gtattggttg gtgctacttc tataggaaga gatatagcgc ctagaattgc
    23761 aggtaaggtt ggaacaggtc taactgctga ctgtacaaaa cttgaaatag attcaacaga
    23821 taataagtta ttacaaacaa gaccagcatt tggtggaaat ttaatggcca ctattgtttg
    23881 tccaaaaaat agaccacaga tgtcaacagt tcgtccagga gttatggcaa aagctgtaag
    23941 aaacgaatca gagactggaa ttttagaagt tgttactcca gaacttactg aaaaaatgat
    24001 tagaactaga ttagtagaga tacttccaca agaaaagaaa tctgtaaatc taactgatgc
    24061 tagaataata gtatcagggg gaagaggatt aaaaagagca gaaggatttg agcttataaa
    24121 agaattggct gataaattgg gggcagagat tggtgcttca agagcagcag tagattcagg
    24181 gtggatagaa cattctcatc aagtgggaca aacaggaact actgttagac cagaactata
    24241 cattgcatgt ggaatatctg gagcaataca acatttagca ggtatgagtg attctaaata
    24301 tatagttgct ataaataaag atgcaaaagc ccctattttt agtatatgtg actatggaat
    24361 agtaggagat ttatatgaaa ttatacctga aatgatagaa tcattaaata ggtaactttt
    24421 agaaaacatt gaatatgtag aatgataagt cttataatat tgtattgtaa gacttattat
    24481 ttttgctgaa ggttatagtg gggagatgaa acaatgaaag agcagtggta taaggatata
    24541 tttgcaagag tattatctat gactgatgat ggttttattg ttgtaaatac aagtggagta
    24601 ataatagata ttaatgataa atattgtgac tttttaggaa aagaaagaaa agatataata
    24661 ggacaaaata tacaaagtat tataccaaat acaaaaatgc tagatgttat gaaaaataag
    24721 tattgtgaag aaggagctat acatcattat tcaggtggaa atacaaaaga aaaaagtgta
    24781 atagtaagta ggtcttatgt ggaaaatgac aatggagaag tagttgcagg agtagctcaa
    24841 gttaaattta gattgcaatc atttgatgta gctaaaaagt taatgtcaga atatatggag
    24901 ctccaatatt ataaagagca gtttaaggat aactgtggtt ttgacaagct cataggtgaa
    24961 aatagagact tcatagaact taagaaaaca ggtgtgaaag cttctaaaac aaactttcca
    25021 gtacttttaa cgggtgaaac aggcacaggt aaagaagttt ttgctagagc aattcacaat
    25081 aatagcagta ggtcagataa acctatggtt agtataaatt gcgctgcaat accagaagaa
    25141 ttattagagt cagagctatt tggctatgac gaaggagctt ttacaggagc aaaaaagggt
    25201 ggtaaaaaag gtaaattttt agttgcgaat aatggtacaa tctttttaga cgagataggt
    25261 gacatgcctt tgaccatgca agcaaaactt ttaagagttc ttcaggaaag tgagatagaa
    25321 ccagtgggag gtcttaaaac tataaagata gatgtaagag taatctctgc cacaagaaag
    25381 aatttaagta aaatggttga agagggatta tttagagaag acctatacta tagattaaat
    25441 gtaataaaca ttcatatgat ggaattgaag gacaggcaag atgatatttt attgctagca
    25501 aattatatat taaacaaatt aaatgttgaa tacaaggaat tgaaggtttt aagtgataag
    25561 gttaaaaatt gttttattaa ctatacttgg ccgggaaata taagagaact acaaaatgta
    25621 ataaaaagtg cctatgctgt aagtgatgat atggtcatta tgatgtgtga ccttccatct
    25681 aagatggata atatcagcag agtagctcaa tgtaatgtgg atagcaattg ttcaattcat
    25741 gaaatggttg agaattatga aaagagctta attattgatg tgttaaggaa gtataattgg
    25801 aaatgttcta aggcagcaga ggttatggga atacacaaga gtcttttata taagaaaata
    25861 aaaaagtatg aaattgaatt aaataattaa gataacttga attaaggcaa tgctaaaaaa
    25921 gcgcccttat tcaaagcgaa ctgtataaac tgttttatgc agttaaaacg tgtaaaagta
    25981 gatgtgaagt actaatgtca atcattagta cttttttatt atatttataa ttactatcta
    26041 tatttcagta tcttttgata tctaagatta ttatgtttaa tttagtatat aacaaaaccc
    26101 tcttagttat ggacagtttt ttatatttta actgtctact aagaggggat agtttagttt
    26161 aaatttagag taaattttat ctagcattta taaaaaatat tttgaatagc ttttttgtta
    26221 tttagtcata attttctact tctttatcaa gttctttgcc acaagaatca cacttatata
    26281 tagcattgta tccgtaatca tattcatcaa ctattttctt ttgtctaaat acaccaccac
    26341 actcacatag gaaaccaatt tttataagtg taacgataaa ttgtgatttt ataaatttta
    26401 tatcattgca tttatcgtta gtgtaaaatg tataaatata agagttatcg cactcatctc
    26461 ttaattcaaa tctttcaata tatgtgttat tttttaattc ctcaaattct tctttagtta
    26521 aatatgattt ttgttgcaat tcatgtaaac gcttaatttg gtccattatt tatatcctcc
    26581 caataatttt agtattttaa gcttagataa ggaatctaag ttttattaaa tttatcccaa
    26641 ggaattattt aaatcatatt tagttttatt aattacaaca tatataaatt acacgaaaat
    26701 aaaactaatt gtaaaaataa tatcctgtga aaacctaaac tactaacccc ttactaaccc
    26761 gaaaactaaa aaaagaaaat atatccaaat ataagtgata tataaataaa gatgtttcat
    26821 ataaatttaa ctttaaatat tctgacaata atatatataa atatattata gttaaaatat
    26881 accacatatt ttgggtattg tatactggaa ttagagtaat taatttattt tttaacatac
    26941 aaatgatttt atgaaaaaaa acaagactca aaaattttta tattctttga tagaataaaa
    27001 ttgagagtaa atttattgaa ttttataaaa tttataaggg gtgagtctaa gatatgcatg
    27061 aagataaaaa tgatatattt gaatcaatag atactgagga ttttgataat aaaacagaag
    27121 aagaatgttt agagctagta agaaaaaatg gattgaattt aatatacata aaaaatcaaa
    27181 cagaagagat atgtctagag gctgttagac aaaattgtaa tgcaataaaa tgtgttcaga
    27241 atcagacaga aaaaatatgt atagaagcag ttaaacaaga ttggagaatg ttagaatttg
    27301 tggaagagcc aacagaagaa atttgcatgt tggctattaa ccaagatgga acagcattaa
    27361 aatatataga aaatcagaca gaggagttat gcttaagagc cgttgaaaaa aatggtgcag
    27421 ctttggagta tgtaaaagaa caaacagaag aaatatgtat agaagcagtt agaaacagcg
    27481 aatttggatt ggcaagagtg aaaaatcaaa cagaaaaaat atgtatggaa gcagttaagc
    27541 attgtagcta taatctaaaa tgggtagaaa atcaaacaga agaaatatgt atggaagcag
    27601 tgagacaaaa tgggcttgat ttgaaatttg taaaaaatca aacagaaaca atctgtttaa
    27661 gagcagtaag acaaaatgga atggcattag aatttgtaaa ggaacagact gttggtatat
    27721 gcttaaaggc agtaagacaa tatggaatgg cattaaaatt tgtaaaggaa caaacagaag
    27781 aaatttgtac agaagctata aaacaagata aaagagcact tagttttgta aaaggagata
    27841 aggaaaaata taaggctcta tatgataata atgaaccatt tgcaaagaga tatgttagaa
    27901 atgtaataga aaaagaaaac agagcattga ttaaaaaagg agaagaaaat gcaaaaatca
    27961 aaattgcagg aaaactttat gatagaggca tgtcttttga agatatatca gatatagttg
    28021 agattgaaat aagcaagtta aaagtgagtt taggtgtttt ttaaagtaat aaatgtaggt
    28081 gttttttatg ggaaatagtt aataaaaaaa taatcgacta gctctttgta ggacagcaaa
    28141 attagtgata taatttgatg tataataaat tatttttatt agataagggg gaattaaagt
    28201 tgaagattac agtattatac caaacaagaa gtggaaacac agaaagggtc gctaagctga
    28261 tagaagaggg cgctaaaaag gtggatggag tagatgtaaa gcttatgaga cttgataata
    28321 ttgatttaga ttatttaaat gaaagtaaag cagtaatatt tggaacacct acttatttag
    28381 caaatacaac ttgggaagta aaaaaatggt ttgatgaaga ttcaaaaaaa gttaatttag
    28441 ctggaaaatt aggagctgtt tttgctacat gtgattatat atgtggaggt ccagatgttg
    28501 cgatacttac tatagtagga catttgatgg ttaaagggat gcttgtgtat tctggaggtg
    28561 gttctttagg aaagccattt atacatttag gacatgtaca ttcaattgaa ggtccagagt
    28621 tacaagatga gaaggctata atatttggtg aaagaattgc tactaaggca aaagaactat
    28681 ttgcttaatt tctatatgca tagtaatgat tagagtaaat ttagggtttt aatggagatt
    28741 tgaagtaaaa gttataaaat aaaaaatttt ccataaaatt actcaagatg aattataata
    28801 taattataaa taaattatat tattaaaata tttaaaatca aacgtcaata gcgagggaag
    28861 tttatggtat ggcatataga atatgagatt ttttctgcaa ttatagttat ttttttaatg
    28921 gtatactttt ttagaagtaa atttatacct actttacaaa acaaaatata ttgtgcactt
    28981 ttaatattct catttttatt tataatttca aatattttag gttcattttg tctaaataat
    29041 atagacaaaa ttccaatttt tattactttt ttattgaatc agatatactt attgttattg
    29101 ccaataccag cagcacttgt atcattttat gttatggcta ttatttatca agatataagg
    29161 tatatgaaaa aaagattatt atttttatct ataccactta taatttgtat aatcttatct
    29221 attacaaatc cttttacgca tattctattt ggattgtcaa aagaaacagg ttatatacga
    29281 ggttgtggat atgttgcaac ttttgcatct tttttctttt atgcaatata ttcagcattt
    29341 ttatctttta aatatagaaa agcaatgcat gaatcaaaaa tatgggctat tagaggtttt
    29401 cttatagttt caattgtggc aattatctta caggcaattt ttttacagta tcttcttaca
    29461 ggaactgcat gtgcatgtag tttacttttg acatatcttt ctatgcagaa tcgaggtctt
    29521 attattgatg accttactgg tgtactcaat agacaatctt ttattcaaga gctagatttg
    29581 aatatcaata ctgaacaatc aggatttatt attacaattg cgcttgatga ttttaagttt
    29641 atgaatgaaa cttttggaac aaaaaatgga gatattgcac taaaagaagt tggaaaatat
    29701 ttgattcaga tacatgatgt aaaccatgta tatagaatag ggggagatat attttctatt
    29761 gttgtcaata gtcaattagg tatagaacca gatgatatta tcagaaagat agaagaaaga
    29821 tttaaaaagc cttggctagt agaagagata agttttaatt tagcaactag tattgctgtt
    29881 gtttattacc cagaaaatgc agaaacgaca gaagatgtaa ttgttgctat agatttctct
    29941 atacatgaag caaaacgctc tgagtcaagg cgagttgttt atgcagatgc gtctataagt
    30001 gaaaagataa agaagaaaca tgttattaaa aattgtttaa aagaagcatt aaaaaatgat
    30061 gggtttgaag tgtactacca acctatattt tctaatagca ggggcaggtt tacttcagca
    30121 gaggcattac ttaggctaga acacaaagag ttaggtttta tacctcctga tgaatttatt
    30181 ccaattgcag aacaaacagg attgattaac tcaattggat tagtagtctt tgagaaggta
    30241 tgtagattta tagctagtga tgagtttgaa gatttaaaat tggataatat agctgtgaat
    30301 ctttctgtag tacaatgtat gcaaaaaaac ttagctgatg atttacttta tcttatgaat
    30361 aaatataatg tatcaccaac taaatttaaa cttgaaatta ctgagactgt ggctatgggc
    30421 tcttttaata taattaaaga aacaatggaa agattaattg atttaggtgt taagtttgca
    30481 cttgatgatt ttggaattgc ttattctgga gttactaata tgctttcttt accattttca
    30541 gtaattaagt tagataagag tttgatttgg tctatgaatg aagatagtag gcataaactt
    30601 acagttgaga cgattatagc acttataaat aaattgaata tgaaggctgt agcagaaggt
    30661 gttgaaacca ttaagtgtgc tgaagattta atagctatgg agtgtgaata tttgcaaggg
    30721 tattattttt ctaaaccagt tccagaagat gtttttaaaa atttgttgaa agaaaatact
    30781 tttacaagtt aacacccttt atttttttaa ttacaacttt gttcattgac actgccagcg
    30841 atatattata aaattaacac aataataaat attaaattta ttatacatat tattaatatt
    30901 aattaatgga gtaaagattt attatatatt ttatttaatt atcctaaagt gagaaaggaa
    30961 gatgtgacat gaataaaaag aaaatattag tgccaattga tgggacagaa agaagtatgc
    31021 attctttgga atttataaaa ggaatattta aaaaagatga agttgaagta gaaataatga
    31081 atgtaaaaga actagtattt attgatggca tatccctggc tgaagagata aaaaattctg
    31141 aaaatttagg aagaagaatc ttggataaag cagcagagat aatgggagag tatgatgtta
    31201 aagtacattt tacttttgga tatccaggag atgagataat tagaaaagca aaagaagata
    31261 atgtagattt tatagttatg acaaaatcta ctaaaaaagg tcttacaaga atgataggtt
    31321 cagtaacagc aagtgtagtt aaacaagcaa aatgtatagt gatgattgta ccggagtaga
    31381 attagaaagg tatttgaaat atgaataaaa agatagtagc agtttgtgaa agtgaaagaa
    31441 gtttaaattc tcttaagaca gctgcaaatg atttaggatt tgatttagtt tatgaaattc
    31501 aagaaaatga taatataaaa aatgaacttt ctatttatga tatagaagat gcatccatag
    31561 tgttatttgt tacaagtgat tctatagaaa gcatagagaa gattgataga tttatagata
    31621 gagaatttta tgaagttgat ccaaagtata taatagagga tgcaaaatct atagtaagtg
    31681 aaattttaat agacttaaat taatcgtggg tgtcgcacta agaatagagc gacgcctttt
    31741 tatatatttt acacaagaaa taaagaggtt gtatcaaaat atagataaga gtctttttca
    31801 ttgttgaaat gatgaaaata tatttgattt catacaaaaa agagtacctc ataactaaaa
    31861 agttcacttt gagacactcc atttttatat gttagttgca tttaaggtaa aatagaagtt
    31921 taatcatgtt gaaataactg aattacatct tctaaatgat tgtacattga tgtataacct
    31981 aagtaagagt ctttactatc aaatgcaatc attatcttgt tatggtggct aatcatatcc
    32041 ttgagagaat ttctatattt aaaaccaaag tattggttag aagaaactgt tttaggtatt
    32101 aactcataat attttataaa aattggatta tgagaagcta aaattatctc tttgtgaaat
    32161 tgcaagtcag catcataaca ttcttctatc aaaccatgag aaaccttatc caattcctct
    32221 attgacttta ggtaagtata ttttattttt tgaagttctt tagatgtagc atttaaaatt
    32281 gctagtttag tagcttcgct ttcgataagt tgtcttgctc ttaaagattc tacataaaat
    32341 tcttgtatgt tatgattttt atagatttca ccatcatcaa ataggtcaac ttttttaaat
    32401 ccttcaacta taggacttga ataaggcaca gctctaagta ttccttcctt acagagtata
    32461 tttatggcat ttcttacagg tattctactt atgttaaata ggtcagcaat ctttctctca
    32521 cttggtaatt tactattaat tttaaaagtt ccatttagaa tgtttaattt aatataatca
    32581 ataatttttt cagtggcatt taagcttttc ataaaaactc ctaaacatat aattttttta
    32641 tgattgtatt atatcatagt taagagttaa atctactttt ttataatatg tataagacag
    32701 gatagaggcc aaaatagcta taatagccaa gataaagtat ccatattgta ttgaagtata
    32761 ttccgatatt ttaccaacta tcattggaaa tacagacatt cctatagcat aaattgcttg
    32821 aaaaaaaccc attgcagtag atttctgatg ttttggtaca tttttcatag actcgctagt
    32881 aaggtaagaa aggagtatac ctgtagatat acctggtagt acttgtagta caaatattat
    32941 attaatattt tttattgttg gaactaaaaa gcaataggct gaaactaaaa taaaaactaa
    33001 aggaatccaa aattttggtc catacttagt acaaaattta gtagaagcaa agccagaaga
    33061 agcaacagca cacaccatgt aaaatataga agatagacct atgagggtag aactagaacc
    33121 taagttttta agaatttgag tagtaaatga cattgttgta gacatttgaa taccctgttg
    33181 aattaatgcc ataaaggaaa aaaatacaag tcttttatcc ttgcagatag ataaatattt
    33241 tttaattgat attttttcag gtattttttt agaattatga ttttttatat ttaaggctag
    33301 aatcgttcca ataactgaag acaatacact catgatacat aaggctgtca ttcctaattt
    33361 ttcataaaat aaagtactca taacaaatcc aatcaacatt cctagattgt ttaccaatat
    33421 tatcgtactt gtagcttttt gttgatgttc tgtagaaaaa aagttggtat agtgaaccat
    33481 gtaagaaatc caagttgatg atgatattcc agataaaatg ttagctataa gaaaaccatt
    33541 gcctccagga gaaaagactc ttataagaga tgctaaacca gcaaagaaaa ttccagacag
    33601 tataaatatt ttatgtttat ttatggaatc agcaaatact ccaacaggaa gccttaataa
    33661 acattgtgat attccatatg cacctattat tataccaatc atatttgtag atacattaca
    33721 tgcacttaaa tatggatttt ggaaaggtat atatacatat tgtgaaaacc agaaaaatgt
    33781 aacaatagaa agtaaaagta atttttgatt agatattttt ttcatattat cctccatgtg
    33841 gtatttttat aatgcaaata atataccatt tagatttttt aatcaagtac tttctctatt
    33901 aaaaaaaatt taaatatata attattattt acaaaaaaat tatactttat agttgaatta
    33961 agaaaacgct tatgatacta taaagttaac aaatttgaat atataacagt agacgtgtta
    34021 ctggtaatgc aggcaagatc gaatcaattt ttatagatta tctatagagt actatgggta
    34081 taatctattt atttggttcg atttttttgt ttaattggcc ataaaacaaa aagcctatag
    34141 ttgaaaatgg aggatttttt atggattata aaaatatagc tcaagaaatt ctactaaatg
    34201 taggaggaaa agagaatgta aatgaagtga ctcattgtat gacaagactt agatttaaag
    34261 tcaaaagcgc ttctaaagtg aataaggata aattatctaa aactgaagga gtaataactg
    34321 ttgttgagag tatgggtcaa atacaagttg taattggaaa taaagtaaaa aaagtatatg
    34381 atgaggtcat aaaaattgta cctcaatcaa ataacatagg taaaaagaat gaaagtcaag
    34441 aaaagcaagg tataattaat tctatattat cagcagtagc tggtatattt acacctacaa
    34501 tacctgctat agcaggggtt ggtatgatta aaggtatttt atcagttctt gctatgtatt
    34561 atatgaataa aaatggagta gatataaaag aaacgcaaag ctatattatc ttaaatgcta
    34621 tggctgattc tatattctat tttatgccta ttatactagg atatactgcg gctaaagtat
    34681 ttaatgcaaa taaaataata tctatggtat taggagctac actttgttat ccaactttta
    34741 ctgctttgat ggcaggtgaa gaatctgtta gatttttagg attggcagta acaaaagcta
    34801 cgtatacatc atctgttatt ccaataatta ttgctatatg ggctctttca tacgttgaaa
    34861 aagtattaga aaagtatata cctgaggtaa ttaaaataat aatggttcca actttatcat
    34921 tggtagtaat gttacctgca acattatttt tatttggacc aatcggaatt tatataggaa
    34981 atgtcataaa cttttcttat aagtatattt atgaattaag cccagcattg tgtggagcat
    35041 ttgtaggtgg gttatggtgt gtgttagtta tatttggtgc tcatagagca ttattaccaa
    35101 taggtataag tgatgttgct caaacaggaa gacaaaattt attggcgttt gcaggagctg
    35161 caaacttttc acaagcaggg gctgctctag gagtattctt taaaactaaa aatcaaggac
    35221 taaaaacaat ttctatgtct gcaactataa cagctttatt tggtataact gaacctgcaa
    35281 tatacggtgc caatcttaga ttaaaaaaac ctatggtatg tgccgtaata tgtggtgcat
    35341 ttggaggtgc gataatggga atgggtggag cttatggtaa tgcatttgca aaccaaggtg
    35401 ttttaactat acctgtatat gctgaagcag gtgcattagg tttcttaagt tatctaggtg
    35461 gttgtgctat agcatttttt ggttcagcta tttcaactta tctagttgga tttgaagatt
    35521 tggaagagtc ttctaataag gaaaattcat ctataaaagt agaaacgaag gatggtgtta
    35581 ttgatataac ttcacctgta gagggcgaat gtatagaatt gtcagaagta aaggatgatg
    35641 tatttgcttc aaaagctatg ggagaaggta tagctgtgtt acctacaaaa ggagtaataa
    35701 cagcccctac tgattgtgaa gtagcttctt tatttccaac attacatgca ataggattaa
    35761 aattagataa tggagcagaa atgttaatac atgttggaat caatacagta gagttaaatg
    35821 gtaaatactt tacaaaacat gttaatcaag gagatttagt gaaaaaaggt gataaactta
    35881 tatcgtttga tatagataaa ataaaaaagg ctggttatga tgtaactacg ccagttatag
    35941 taaacaatac atttgatttt gggcaagtag tttcttgtaa atctagttat gtttcaacca
    36001 atgataatat aatttcatta gtattagctg gtaattaagt taatttgact agagataagt
    36061 aaattgattt aaaaggaaat aagtaaatca acaattaaat acatctaaaa gtaagtttat
    36121 tgcaatgaat tgttaaagaa aataattaat atggagtaaa tctattgcaa tgaagtaaca
    36181 aagaaaataa ttaatataga gtaagtttat tgtaatgaat tgttaaagaa aagagttaat
    36241 atagagtaag tttattgtaa tgaattgtta aagaaaataa ttaatatagg gtaaatctat
    36301 ctcaatgaaa taacaaagaa aagagttaat atagagtgaa cttattgcaa tgaatcaaca
    36361 aagaaaataa tcaatataaa aaggagaaat atttatgaat acaggatttc caaaagactt
    36421 tttatgggga gcatcatctt ctgcatttca agtggaagga gcttgggata aagacaataa
    36481 aggaaaaacc gtagccgact ataattcttt taaaaagtct catttacagg cagatactaa
    36541 agtcgcatca gacttttacc ataattatga agaagatatt gaattaatga aagaattagg
    36601 gatgaaaaca tatcgttttt caatttcatg ggcaagaata ataccagatg gtgaaggaga
    36661 gataaaccag aaaggtttag aattttataa taagataata gacaaactga tagagtgtga
    36721 tattgagcca ttcgtaactt tataccattt tgaccttcca tttaaattag tagagaaata
    36781 taatggttgg gaaagtagag aaactgtcta tgcttttgaa agatttgcta aaatatgctt
    36841 taagcatttt ggagataggg taaagtattg gcaacctcat aatgaacaga atttaattgt
    36901 tagagtggag gagagaatta atatatatga tgaaacagat tcatggaaga tagataaaat
    36961 gcgtgctcag atggattata atctttgctt agcacatgca ttagcagtca atgcatgtca
    37021 tgagatgata aaagaaagta aaattggagc agcagtatca tcatctgtaa catatcctct
    37081 tacttctaag ccagaagatg tatatgcagc tagaatgaat gataatttta aagtgtacta
    37141 tatgcttgat atgcatcatt atggagaata tccagggtat tatatgaagt atcttgaaaa
    37201 aagaaatata gttcctcaca tggaagatgg ggataaagag atacttaaaa aagctaaaat
    37261 ggattttata gctgtaaatt attataggac aaattgtgca gaagctttac ctgaagattc
    37321 acaacatcca tttggattaa gagaaggaac tgttgatttt agtatgtatg gactatttaa
    37381 aatgtcaatg aatcctaact tagaggcttc tgagtatgga gcagcaatag acccatcagg
    37441 tcttagagtt gccttaaatg aatattggca aagatatcac ttacctgtta taataacaga
    37501 aaatggtcta ggagctaaag atatattaga agatggaaaa attcatgatg actatcgtat
    37561 agactattta agaagtcata taaatgcatg taaactagct attgaagatg gtgtagagat
    37621 gattggatat tgtccatggt catttacaga ccttttaagt tcttctcaag gttttaataa
    37681 gcgttatgga cttgtttata taaatagaac tgaccatgaa gttttagatt taaaaagaat
    37741 caagaaagat agtttttact ggtataaaga agttatagag aacaatggaa tagtaaaata
    37801 aacataaata tatattgagg gttttagtat gtacattaca aaggtattaa ataattcgtt
    37861 gctacttgca aaggatgata atgatgaaga aataatattg atggggaaag gcattggcca
    37921 taattataag gccgggtctg agttaaataa aaaagatata gaaaagattt ttgttcttca
    37981 tgatgaaaat ataaaaaaga gtattataca attggcaaca gaaatagatg cagaatattt
    38041 tggtatagct caaatgataa ttgcatatgg aatagaaaaa tataacttaa aacttatgaa
    38101 ccatatatat ttggccttaa ctgaccacat tgcctttgca gtgagacgtt ttaaagaagg
    38161 tataatgatt gaaaatcact atttgttcga aatcaaggaa tttaatccaa aagagtatga
    38221 tataggaaaa tatgctatta ctgtttttaa ggaggttctt ggtttagacc taccagaaga
    38281 agagattggt aatatagcag gccattttat aaatgcacaa caagataatc cttatagtga
    38341 tagaaataag agaagtgcta aaattgtaaa tgctattttg caaatagttc attatcactt
    38401 ttctattgta tataataaag agtcatttta ttataggaga tttgtaatgc atctaaaggc
    38461 cttttcacaa agatttctgg ctaatgaacc atcaagagaa aaaatagatt ttatatatga
    38521 gcaagttcaa aaaaattgta aggaagagta tgaatgtgtg aaaaaggtag gagcttatat
    38581 gtataaggaa tactccagag aattaccaag acaagaggag ttatatttga tgattcatat
    38641 tcataagatt ttaggagaat tatgcgaaaa tagttagaat atcttaatat gctagtaata
    38701 ttataaaaaa ttaaaaatta actgcacttt aaatatgaat aattgcatta cttaaggggg
    38761 ttgtcttaaa attgattttt ttaattaaat ttgaagctca actccttttt taaaccttta
    38821 tatcattaca actttaatgt ttatacgact gtactttaga tgtatatcta ctttttacat
    38881 ttctcaacat gcaaacctac cattttttgc agtatgttaa ttgaaataaa ccattgagtt
    38941 aaccctaaat ttattctgga cttataggaa tagttccaaa gatactttaa aatattttct
    39001 ggaataattg cttatttcac atatgtttta ttgtgatgtt ttgatttaat aaattcttca
    39061 actctgtata tagtaaatgt attatcatgt ttgttttatt caagcacact tataatacgc
    39121 ttgatttttt cacaatgatg aaatatacta atgggtgtta taggtataag aatcgcatta
    39181 cacttgaaga agtaagtgat aaatgaggta taaatcctca tatgataaac tactatttca
    39241 ttgaggaaca tattctaagt gatgtcaaga tactacaata tgactaaatt cctagtgata
    39301 aaaaagtcta cgaatgagtg tagaaagggg aactaagatg aataaagttt taattataga
    39361 tgatgataaa gatctttgta ttttgttaaa aaagagtatt tcaattgaac agattgaagc
    39421 aaactattgc cattctggga gagatggact ggttgaactt tcaaaacaag attatcagtt
    39481 agtagttcta gatgtgatga tgccaggtat ggatggtttt gaaactatgg aacaaatacg
    39541 aaaatatagc agtattccca tcttaatgct gacatcaaaa agcgataatt acagcaaagt
    39601 gcatggattg cgagctggtg cagatgacta tctaaccaaa ccatttgaaa ttacagaatt
    39661 tactgctcgt gtactgtctt taatccgacg ttacacccgt tttaatccaa aaaatgagga
    39721 gccacagcaa ctttctttca aaggtttgtt gattgacctt gaccagcata cagttactac
    39781 caatggcgaa cctatgttgc ttccacccaa ggaatttggt gtacttcttt actgtgcaag
    39841 gcatcagggt aagattttga caaaacagca gatttatgaa gccgtgtggg gtgagccata
    39901 tgtatatgat gacagcaata tcatggctat tatcagtcgc ctaagaaaaa aaattgaact
    39961 agacccatca tctcccatct atttgcaaac aataaaagga attgggtatc aattcaacac
    40021 ggaggtatga aaatggatga acaaacgata attcttttct tactcttgct gtcgattggt
    40081 ctactttttc tttccatatt tgctttttcc aagcttgggc atatttacaa gcgcctaaaa
    40141 gatatagagg agatactggc tgatgtacaa attggagacg aaaatcgtaa aatactaatt
    40201 aagccatgtg atgttatggc tccattggtt tatcagctta atgagattgt ctacgattat
    40261 gaaaataagt tactttctct gaaaaagtcg gataaagcta gcaaacaact tatgacaagt
    40321 ttatctcatg atgttcgtac accattaaca actctgattg gttatctgga tgctgtacac
    40381 agtggtatag ttatcgaaca agagcgggag gaataccttg aaatcgctag gcgtagagcc
    40441 tacgacctca aggattacat tgatgttctt tttgactggt ttcggttgaa ctctgatgaa
    40501 tttacgctgt caatagaatc tgtggaaatt gcagagttgt cacgcaatat tttgaaagac
    40561 tggattccta tattccatga aaagaaactg aattttgaaa ttgatattcc agaaaatcga
    40621 ctaatggtaa acctcgaccc agatggatat tcccgtgtgg tcaataatct ggttcaaaat
    40681 gttcttgctc acagtaaggc tcggcaaatc aagattacca tgagtgagga ttccagaatg
    40741 gtattactcc gtgttgagga tgatggtgtt gggattgcca gagaaaattt accacatatt
    40801 tttgagcgac tgtataagtg tgataaagga cgttcagaaa aaggaagtgg tcttggtcta
    40861 tctattgtca gtcaaatggt ggagcgaatg ggtggtcagg tttccgtaga aagtgagatt
    40921 ggaaagtata ccgtatttac agtgagctta ccattaatat aaaattgttt ttcccccatg
    40981 tgacatgtat acacatgggg attttttgta agttataaag ttgcaaggtt aatacaaggt
    41041 tgttgcaagg ttaatgtaag attgaggtgg tatgataact ttaataaaca aaggaggctt
    41101 ttattattta tgaataattt aattattgaa acaaaaaatc tcactaagca atatggtgct
    41161 caaaagagcg tttctaattt gaatattcac gttcagaaag gtcgcattta cggattgcta
    41221 ggaagaaacg gtgcgggtaa gacgacaact atgaaaatgt tattgggtct aacaaaacca
    41281 actactggag aaattaaaat atttggtcag gacattcaca ataacgagaa gaagcttctt
    41341 cctcgaattg gatgtttgat tgaatcacca gggttttatc ctaatctgac aggaacagaa
    41401 aatctcaaaa tctttgcaag gcttagagga ataccaaaaa aaaatgcagt agaatcagca
    41461 ctggatgtag tcggtttgcc aaacaatgac aaaaagttat tttctcaata ctctcttggt
    41521 atgaaacaga ggttggcgat tgctcttgcc attatgcatg acccacaact gttgattttg
    41581 gacgagccta tcaatggact tgatcctatt ggtattgcag aagtaagaac gtttattcgt
    41641 gatctctgca cagaacgagg taaaactatt ttgatttcca gtcacattct ttctgaaatt
    41701 gccttgttgg cagatgacat tggcattatt gaccatggta ttttgttgga ggaagaaagt
    41761 cttgcccaat tagaagaaaa aaacagtaaa tacatacatt ttgctgtatc tgatgcagca
    41821 caggcagcaa ggcttctgga aacccaactt aacctgaaaa atttcaaagt agcagataat
    41881 tacaatatta tgctatatga tacagatgtt tctgttgcag aaataaatcg tgctttcatt
    41941 ctaaacaaca ttgaggtatc agaatctcat ttatgcgaag atactcttga agattacttc
    42001 aagaaagtaa cagggggtga aggaattgct tgatataatt tcatgtgaat tttcaaaact
    42061 caagcggtta aaatttatac tgatttctat tttaggtgct tgcttatttc caattcccgc
    42121 aacaattttg attgcaaagg acaacttgcc ctttgagcaa ttgttcaaat tagttgtcaa
    42181 ttttggatat tttttgctat taccaattgt attaagtatt gttgctagcc aactattttt
    42241 tatagaaaga gataatgatg tattaaaaaa tcttgcaaca gtgccagtgc aaaaaggaaa
    42301 actggctctg gcaaaattgg ctgtgttgct gtttatatca ttgttctact ctgttgcagg
    42361 gttaggagct actatcattg gaggtttcat tgttggaata gtagaggggg ttgctgtaaa
    42421 attaggaatg agcattgctt ttggaattat gttttttgtt actgtacttc ctgttgtggt
    42481 actcattgta tatttcaatc gcagctatat tttttctatt atactttctt ttgtatatgc
    42541 tattttcaat tttagtatat ccttaaatat tatcaatttt gaaccaaata acccaatatt
    42601 aagtgtatta cctgctcctg ttataatgcg ttggtggatg gcattttggg gagatcccac
    42661 tggcgaatac acggcactta gacagccata tttgctctca actcctgctt gtgtaggtat
    42721 tttatgtttg ataaccatta tagcagtcct gctgatttgt atgatataca aaaagcagga
    42781 gaattaggag gtatctatgc ttgatataat tactacagaa tttcaaaaaa taaaaagata
    42841 taatattcta tggattggtg ttgttgctgt tctgttttca gcactattgg cagtttttca
    42901 ggttgtaagt tcacacggca gtgaccctct aacgtatgag agctttgcaa acggagttat
    42961 ctggaataat ttcagtctta gttttccatt tgggattaca cttattggag gatatcttat
    43021 taaccgtgaa tatacagacc aaaccttaaa aaacatccta actgttccta tttcattacg
    43081 caaattgcta gtgggaaaat taattgcggt tggaggaatt actgtattgt taagtctttt
    43141 cagcttctta tgcacattta ttttggggtt gttgttttgc aatatcaata tatctgttac
    43201 actcgttgta aaatccttag ttcagatttt gtctgttaat ctgtattgtt atgttgctgt
    43261 tttgccgatt attgcttatt ttggtcgtaa acaaaatgca tttctgacgg gtgtagggat
    43321 tgcatttgta tacggttttt gtggtgtgtt tgttgctgga agaaatctga cagactttta
    43381 tccaatcaca gcaggtcttg gtctagttgg atacaacaat ggtgctggta ctgtttatca
    43441 gccacttatc ggtgctatga ctttgattgt aattctaatc ttgacaacta ttctacttgt
    43501 atttacacct aattatgata aagttatgac catatctaag aaaaaaggga taaagcataa
    43561 aaagtattct cataaataac ataaatattg tttctattat ttaagttgct agacagtgaa
    43621 aagaaaaaat ctgtcgtaca gtgtctatat gctcatgtat tcaagaagct acagttttga
    43681 tttttcataa ctgtagcttc tttttgtatt tattaaatca cgactatcat aatacttgca
    43741 ataggtatag cgacaagaga gagtactgtc atatgtgttt tatatattac attaagtata
    43801 ttctaaaata tatgttttaa ataatatatt tgaaaatagg aatatttaaa taaaaaaaat
    43861 aatattatat atagttgaca aaaattggca aatgagataa tatataaata tagaaatagt
    43921 aatcattact attattaaaa agggaggaat taattatgaa cttaaaagga actaaaacag
    43981 aaaagaattt aatggcagga tttgcaggag agtcagaagc aagaaataaa tatacatact
    44041 acgcttcaaa agcaagaaaa gaaggttata atcaaatagc tgctatattt gaagaaactg
    44101 ctaacaatga aaaggaacat gctaaattat ggtttaaact tttacatgat ggtatgcctt
    44161 caacagagga aaatttaaag gatgcagcag caggtgaaaa ctatgaatgg actgatatgt
    44221 atgcaaaatt tgctaaagaa gctagagaag aaggatttga caagatagct tatttatttg
    44281 aagctgttgg aaaaatagag aaagagcatg aagaaagata cttaaagctt ttagaaaact
    44341 taaatgaagg aaaaatattt aaaagagatg aagaagttgt ttggcaatgt caaaactgtg
    44401 gtcatgtgta tgtaggtact gaagcaccag aaaaatgccc agtatgtgac catccaaaag
    44461 cgtatttcaa tataaaagct gaaaattatt aaaaattatc attattaaaa aggaatgtta
    44521 actatgaaat tttctaaaca acgagaactg attttaaatg aaatattaaa taatccggtt
    44581 catcctactg cggattacct atatgaaaac ttaaaaaaag ataatccaaa tttaagctta
    44641 ggaactgtgt atagaaattt agctcagcta acggaacatg gctttataag aaaggttagt
    44701 attccagggt atccagatag atttgatggt cgaatagata atcactatca tatcatatgt
    44761 gaagtatgtg gagaggtata cgatttagaa tccgaagttc ttaataattt acaagaatta
    44821 atatctgagg agacagacat aaaaataaca tcttataaca taagttttaa agggatttgt
    44881 aataattgta aaaggtgtag ccaagtaggt tagttttcaa attctaaaaa taattaatga
    44941 ggtgagttta tgtgtagtga acaaaaattt tttatatgtg agacttgtgg aaaccttgta
    45001 ggaatgatac aaagtggtgg tgtacctatt ttctgttgtg gaaaacctat gaaggaactt
    45061 gttcctaata caacagatgc agcagttgaa aaacatgtac ctgtaattga agtggatgga
    45121 aataatgtta ctgttaaagt aagtagcaca actcatccaa tgactaaaga acatcatata
    45181 gcatgggtgt atcttatgac tgaacaaggt ggtcaacgca aatgtctagc agtagatgga
    45241 gaacctgtag ttaaatttgc tttgaatgat gatgataaag tgatttctgc ctatgcttac
    45301 tgtaatttac atggattatg gaaggctgaa ctgtaatatt taaataaatt gagtcaaaaa
    45361 ggaggctata aatgtatttt catacaaata gcctcctata aatttatata aattcaagtt
    45421 ttaattttgt ttaatgtcaa tataatataa ggagaaaata atatgtcaat ttataaatgt
    45481 agtgtttgtg gatatattta tgatgaatct aagaatgata aaacatggga tgaattaagt
    45541 gaagattggg aatgccctgt atgtacaaaa ggtcgtagtt attttggaaa gataagtact
    45601 gtttactatg aagaagatga aaagatagca gaagacatag ttgaagatga atctaaatta
    45661 aatactgaaa aagagggaga tttaaattac ttaagtacat atttaagaag agatgatgaa
    45721 gttgagaaac atatggatat aattcacgaa atggctgtta caggaaaatc tataatagaa
    45781 cctatgagaa ctaaactacc agtaatttct tgggatgata ttttaattat gggtgctcaa
    45841 ttaaatccat tacctttaaa tgagcatgat gaagtaaata caacaacaat aataggaaaa
    45901 aaagcaaaga aacctatgat tatagaaaat ccagtttata tttcccacat gtcatttgga
    45961 gctttgtcta aagaactaaa aatagcttta gcaaagggtg cagcccaaaa taagacagct
    46021 atgtgtagtg gtgaaggagg tatattacca gaagagaagg aagcttcata caagtatatt
    46081 tttgagtatg taccaaataa atatagtgta actgaagaaa atcttaaaaa ttctgatgct
    46141 atagagataa aaattggtca aggaacaaaa cctggtatgg gaggtcatct tcctggcgaa
    46201 aaagtaactg aagaaatagc aaaagtaaga aataagcctg taggtcaaga tgtaataagt
    46261 ccatcttgtt ttgaggaaat acaatccaaa gaagatttaa agaaacttgt agatgaactt
    46321 agagaagttt cagaagggcg tccaataggt gttaaaattt cagcaggtca tattgaaaaa
    46381 gatatggaat ttatagctta tgctaaacct gattttgtaa ctattgatgg tcgtggtgga
    46441 gctactggag caagtccaaa gttgcttaaa gatgcaactt caattccaac aatatttgct
    46501 ctttatcgtg ctagaaaata catagataca catggattag atatagattt ggttataaca
    46561 ggtgggcttc gtatatctac tgattttgct aaggcaattg ctatgggtgc tgatgcagtt
    46621 gctattgcaa gttctgctct tatggcagcg gcgtgtcaac aatatcgtat atgtggttct
    46681 ggtaagtgtc ctgtaggagt agctactcag gatgaggaat tgagaaaaag attacatata
    46741 gaaaacagtg ctaatagagt ggcaaacttt ttaaatgtaa gtttagaaga acttaagact
    46801 tttgctagaa tttctggtca taaagatata catgatttaa gtgttgatga tttatatact
    46861 gttaactctg aaatatcaaa ttatacaaac atacagcatg tatagattga agtttgaaac
    46921 tattagattt tagatagcta taaaagtaaa tgataaatat attttataaa agagagtgtc
    46981 ttaaaatgaa ctttttagtt cataagtcac tctttttttt atgaagccaa atatatttat
    47041 cattttagca atgaaaaaga atcttatctc tttattttga gatagactcc ttctatttat
    47101 gaaaattaaa gatattaaat tgtaaaatat tctgtgatgt cgtactatta aactaaagat
    47161 tctgtacttt aaagtaaata aaaatttaag gagtgattat tatggaaaga ttgaagatac
    47221 aagttttagt tgacaataat acttatattg atagatactt tgttggagaa ccagcagtaa
    47281 gctattatat tgagattgat ggaaatagga ttttgtttga ctcagggtat tcagatgtat
    47341 tcatatcaaa cgctgaaaaa ttaaatatag atttaggaaa tttaactcat gttgtttttt
    47401 ctcatgggca taatgaccat acaagaggta tccagtttct agaaaatagg tatgatttat
    47461 ctacagttga acttatttca catcctaact gttttatacc tagaaaacat ggagagaaaa
    47521 gcataggagc tccattttct gctgaggaaa ttaaaaatat ttttatgtat aatcctaagg
    47581 ataagccatt taatctaagt aagaattgtg tgtttttagg tgaaatacct tctataaatg
    47641 attttgaaaa aagagctaaa attggtaaat gtaaaatagg agatttgtgg gaagatgatt
    47701 atgttttaga tgattctgct atagtttgta aaactgataa aggtattttt attgttacag
    47761 gatgttctca tagtggaatt tgtaatatag tagagtatgc taaaaaggta tgtggagatg
    47821 atagagttat tggtatacta ggtggttttc atctttttga attgaataat aggttagata
    47881 gtacaataca atatttggat aaagaagaaa tgagaatgtt gtacccttgt cactgtgttt
    47941 cttttaaggt aaaagcgaag atgaatgaaa tattgaatat aaatgaagtt ggagtagggc
    48001 tgaccataag tatatagtat ttgaaagtat ataagattta agttgaag
//
LOCUS       DAYAPA010000015        46248 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.015, whole genome shotgun sequence.
ACCESSION   DAYAPA010000015 DAYAPA010000000
VERSION     DAYAPA010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 46248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46248
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.015"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            465..587
                     /locus_tag="ACGI59_000755"
     CDS             465..587
                     /locus_tag="ACGI59_000755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9738561.1"
                     /translation="MMDLEKEKHEIIEIIKKLNIDKDKRNLLMIKNYILGLLKK"
     gene            complement(602..988)
                     /locus_tag="ACGI59_000756"
     CDS             complement(602..988)
                     /locus_tag="ACGI59_000756"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFG9738562.1"
                     /translation="MIDEMKPENKRIRELRLNLNLSQEEFANKLNLKRNTISLIENGK
                     RNASERTLNDICKTWSISEEWLKHGVGEMFFIKELDEQFAHLVGELSKMENEVEREII
                     TKMFKLNKKYLTVFDKMIDSLLEIEE"
     gene            1136..1279
                     /locus_tag="ACGI59_000757"
     CDS             1136..1279
                     /locus_tag="ACGI59_000757"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9738563.1"
                     /translation="MTDINKIREEIYRKLELLGPAKLKLALTKVDILKEIQDIEDENN
                     IQN"
     gene            1284..1508
                     /locus_tag="ACGI59_000758"
     CDS             1284..1508
                     /locus_tag="ACGI59_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9738564.1"
                     /translation="MKRLKISDFNKNQIGSAVQLQDIRFDTKADCIITKVEKNEILVM
                     YYEKETEEIAYKTLTKEDLILDDYKLKLLS"
     gene            1527..2267
                     /locus_tag="ACGI59_000759"
     CDS             1527..2267
                     /locus_tag="ACGI59_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFG9738565.1"
                     /translation="MITLDLLKDARKKEWFWLENDLIDREDLSIYEKMIYIVLARYSN
                     EESSCFPSYKTISEKAGCSIRQVSNILNELENKELIIRENRNHEGKKEKNSNLYFLIS
                     SKAKVQNEVPNVRHEVPNPMAPGALHVRHEVPSKKTYNKKTYIKSNTTTQKESKTDYL
                     DLSFLDLDIEKVKLTKDEYDKLISKFGKKYIHDKIVSLENYIINGKGSRYKSHYRALL
                     TWGNGDASKGIVPEVTKTKNPLSGFKEL"
     gene            2295..2480
                     /locus_tag="ACGI59_000760"
     CDS             2295..2480
                     /locus_tag="ACGI59_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9738566.1"
                     /translation="MNEKEETVYLNSSNVKLFLGVRDIKEFEELIENVNERICQLQES
                     INKLAQFNIEFDIKSDI"
     gene            2506..2724
                     /locus_tag="ACGI59_000761"
     CDS             2506..2724
                     /locus_tag="ACGI59_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9738567.1"
                     /translation="MKDEKLELTEKDLHCIARHLQNEVMEMVFRGNIEAPTSCEVCDY
                     LQECKDYFTHIDTFIKLSEMTGVDIFTK"
     gene            complement(2743..2949)
                     /locus_tag="ACGI59_000762"
     CDS             complement(2743..2949)
                     /locus_tag="ACGI59_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9738568.1"
                     /translation="MCKIKTLREKCSLIDDKVEFSGEYKTFPGFTGSKLLECECNKNY
                     ICKIQIMTANSNCPLFKKARQLQY"
     gene            3118..3378
                     /locus_tag="ACGI59_000763"
     CDS             3118..3378
                     /locus_tag="ACGI59_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9738569.1"
                     /translation="MKEEKLELTEKELHCIARHLQNEVMEMVFRGNIEAPTSCEVCDY
                     LQECEGDFTCMYHSFTKLSKITGVKVTAFKYCFPDENPKATI"
     gene            complement(3371..3559)
                     /locus_tag="ACGI59_000764"
     CDS             complement(3371..3559)
                     /locus_tag="ACGI59_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9738570.1"
                     /translation="MSQYRVISKECPVINETISLTCEYSAPPGTFGSKLMSTRCDGSH
                     ICDSSSNEECPILKKLLR"
     gene            3697..4014
                     /locus_tag="ACGI59_000765"
     CDS             3697..4014
                     /locus_tag="ACGI59_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9738571.1"
                     /translation="MERKKHEFTDQDIQRLIEGLRTDPKIREMIFKSKGCETRTSTRS
                     KCEQFVEEKLAYRSYGFETPKYYAKLIADIIDILVYKNVPRDRICGVLYDVERVIPLV
                     VTF"
     gene            4036..5367
                     /locus_tag="ACGI59_000766"
     CDS             4036..5367
                     /locus_tag="ACGI59_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase N-terminal domain-containing
                     protein"
                     /protein_id="HFG9738572.1"
                     /translation="MNYSLEAEQNILGSFLINDSIGYKIRELKENDFYFESHKIILSC
                     MKKIIDSNKPLDLLLLKNELEKIDRLVDIGGVSYITSLTTIVITTSNIDHYIKIIKEK
                     LLKRQISELANELINNSNSDTSIDELIIDINDLKALVTTSSSVNDNYIDASKIKREKG
                     VHKSIDTGFNKLNNMLDGFRYGTLTILTGKPASGKSTIVNQFIAQAITNGEKAFLYSG
                     ELPSFMSMDWFRKTVANDYHIKEYKSVYGGTYTDIPDYAVELISDWIEDKFFLYDEDA
                     ISDEVNLLNTIEHLYLKKGVRFFVLDNLMTIKTGNKADKYERQEQIVSNLKNLAKKYN
                     LVIVLVAHPRKNMGDMKPTMYDVSGASEIVNYADYILSTYRVVDEEEETDDTYLLILK
                     NRITGKQNISFKMNFSERRKRLYTTSEELNRDYKYDVNKQYVQVEIPEDVF"
     gene            5742..6128
                     /locus_tag="ACGI59_000767"
     CDS             5742..6128
                     /locus_tag="ACGI59_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9738573.1"
                     /translation="MIALEKFAGGVLKEKFNTELQKVLDNIADPNTDFKKTRKISLEI
                     VFKANEDRDLAEVDIKSKATIVEAKATTTKVLIGKDLETGRVEASEFKNQVAGQLVMD
                     IPDSEETNETEENSAGVIDFRNASSK"
     gene            6181..6885
                     /locus_tag="ACGI59_000768"
     CDS             6181..6885
                     /locus_tag="ACGI59_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9738574.1"
                     /translation="MIRNAMEYLVKLGKRETVCVDGFKYTTDALEIIKEPKANELEIT
                     TLSGLVDYIKSGIDHKADELLLIQVLTPRKVLLKSPLRKNRDRETYIECVALLPEKRF
                     DRPLDLDSFNIMLQSAFIKNNDRDTLLKIAGNIQESTVKNVGDDGVSQSVTIKTGVAS
                     VSEAIVPNRVKLIPYRTFQEIEQPESEFIFRMSSGPAAGLYEADGGAWKNQAMLNIKA
                     YLQEELKDFKNVNIIA"
     gene            6942..7124
                     /locus_tag="ACGI59_000769"
     CDS             6942..7124
                     /locus_tag="ACGI59_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9738575.1"
                     /translation="MKMISHMNILERAEFTDKIANAIMSANKEIERGCAFNEAVEIVK
                     QMEYRENKYNEGDIAI"
     gene            7121..7498
                     /locus_tag="ACGI59_000770"
     CDS             7121..7498
                     /locus_tag="ACGI59_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFG9738576.1"
                     /translation="MNNVVLVGRLARDPELRYIPEYGTPVATFALAVDRGYTKKDGTR
                     EVDFIPIEVMGGSAEFCANYLAKGRMVSIQGQIRIEKYEKDGEKKTFTKVRTKIVNAL
                     DRKPKDEEKEISFQVLDDEDIPF"
     gene            7515..8063
                     /locus_tag="ACGI59_000771"
     CDS             7515..8063
                     /locus_tag="ACGI59_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFG9738577.1"
                     /translation="MRSLEERNKLVEENINFVWFIIRRYFKVFISKYPYLQDDTFSEG
                     CIGLCKAADYFDSKKGIEFTTFAAVCIKGQIFKFISRYVKKHYRADIDSFEKCIYKNT
                     HGEEIRIEDRLAGNENVDIENVHVIRSCIKRSKIEDIQKITYLREKGYTQKEIGKILR
                     TNQMTICRKLGKLKSEINILGK"
     gene            8093..8251
                     /locus_tag="ACGI59_000772"
     CDS             8093..8251
                     /locus_tag="ACGI59_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9738578.1"
                     /translation="MKTKIIGGRENQCPLCNGSVFKIETSIGIVWQCKDCGCMYQDMN
                     CKENKRRH"
     gene            8254..8580
                     /locus_tag="ACGI59_000773"
     CDS             8254..8580
                     /locus_tag="ACGI59_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9738579.1"
                     /translation="MACVIKCDFCGETIAEILNIKVFRIDDEGNIMSIGKDVCDKCYD
                     RLFRSNLNKKMTNYEKIKNMSKIEMAEFLANGGSGCTSCAYDFQECLGGCLEGRKKWL
                     ESEAMK"
     gene            8580..9116
                     /locus_tag="ACGI59_000774"
     CDS             8580..9116
                     /locus_tag="ACGI59_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HFG9738580.1"
                     /translation="MKKTLIIVGVVISILIMFACLFLVTKNKAIDLEEQVQSATASVE
                     VQEKRRIDLIKNIVDCVKDYAKYENRTLKEVTDMRVSANDESIKEAKLNIQAVAEKYP
                     DLKANENYKQLMSELSITENMIAQHRENYNMQVRNYNKYIRKFPANLILNSMGYEKIE
                     SKYTEYKAKEDAPQNLFD"
     gene            9128..9934
                     /locus_tag="ACGI59_000775"
     CDS             9128..9934
                     /locus_tag="ACGI59_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9738581.1"
                     /translation="MKFKTFEITKREFLVSIIIACMMIVLGFFIDDKVQSKFLEDNER
                     YYKATKINNDKDLFDYAMETSAGDSLISGRFKSVDSVSIEELKGDYWFIERTEEKYTM
                     HTRVVTTKDSKGHSHKYTETYWTWDYHDSIEKKCNKFNFMGKEFDAKVITNLSKERLA
                     LKKDIFKNNNLKIKGNYAYINNKRRYYYEVIPKEFNGTMYANLKNNTIANPADKNIKV
                     FKGESIQNVLENNNFNTGFFKVVFWIIWILITTIIIYVFYYLKNDWLEDK"
     gene            9959..10219
                     /locus_tag="ACGI59_000776"
     CDS             9959..10219
                     /locus_tag="ACGI59_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9738582.1"
                     /translation="MKCKVYHAIKNIKDANELIDRFETENIFFELNDIDNENVLNGEM
                     NLTDIEDIASLQFILDGDLFIFSSCDEMIFMNKYTQKELGLQ"
     gene            10243..10728
                     /locus_tag="ACGI59_000777"
     CDS             10243..10728
                     /locus_tag="ACGI59_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9738583.1"
                     /translation="MDLKLISSIPNWKVEDQLAKIDEEVTEFKNAIDTGITKDIIAEG
                     LDVCQTILTMFQVLEIENYITEGVETHNKKLNGRGWDLKEIGDDILNLIKNSMINELI
                     NMNKKLKCLDEKEFNAKIQFLDVDKLWNLCMPLVNLSTIKGVNESIDEMKMGEFLKNI
                     N"
     gene            10748..11005
                     /locus_tag="ACGI59_000778"
     CDS             10748..11005
                     /locus_tag="ACGI59_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9738584.1"
                     /translation="MLIEVTENNRELKVGDIVEINGGNKLYMILSLKETIGGYVIINM
                     QNGYGSFGAYKSLSQLELDLKVRGYKLYSSDDYKLQLVPRE"
     gene            11114..11557
                     /locus_tag="ACGI59_000779"
     CDS             11114..11557
                     /locus_tag="ACGI59_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma"
                     /protein_id="HFG9738585.1"
                     /translation="MQSSKKDNLFSSAEGKLYDYKKIKAEIEKIDIDMQMIKNDYRGC
                     SGVEIKEKTGKTYDIKSVIEIETEEKEKKIALKEKEKRHKELIIEKIDNAMKILSEEE
                     KKIVQYKYFSSSRTSWEYVGRMIGFSASKCKQMRFDIIDKIKGLL"
     gene            11745..12245
                     /locus_tag="ACGI59_000780"
     CDS             11745..12245
                     /locus_tag="ACGI59_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9738586.1"
                     /translation="MLKIKRDITEEISSAAYVPDNLKYYDNVMKETPGMYGRECSLDF
                     IKKKQEKLLKLKKKVKKNYDSNINKIDSYLKILEKSIIDESDHVELVVFKLYLQLENV
                     VKVTETVNDLGFRIKTNTYARERKYSTNDITSIITDPFTDVVEDLKILVQERQRKNYY
                     GNKECF"
     gene            12249..12893
                     /locus_tag="ACGI59_000781"
     CDS             12249..12893
                     /locus_tag="ACGI59_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFG9738587.1"
                     /translation="MEAHSGQKVNPIKDIEDVFRLLNFLEEWNERNYLLALFGMCTGL
                     RVGDILALKVADVTDLKLDKKGNKIRVSKDWIRVIEEKRDYNREVFLSDVIKSAIENY
                     TQDKPGEEFLFKSGKRKKFNRPIQTRQAGRIIKGAAKKVGIKENVATHSLRKTFARHI
                     YDEEENKTYALELIRKILGHKTIEMTRRYIGIDKDEEVKAITKFTNKLKKRKRN"
     gene            13114..13566
                     /locus_tag="ACGI59_000782"
     CDS             13114..13566
                     /locus_tag="ACGI59_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFG9738588.1"
                     /translation="MNEVEIIDSSYEKGKELTEDQCNMVTLLITGATVTETARIIGVN
                     RKTIYNWMNKEHVRKEMDRRKQELTNQGNLMILKDLDSYINNIKELASDKSDKRVMLA
                     ANQYLINRIYGTPTSTVIQAEDNSVGMGMDATEIEAAIAKIRIRTSKK"
     gene            14121..15860
                     /locus_tag="ACGI59_000783"
     CDS             14121..15860
                     /locus_tag="ACGI59_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9738589.1"
                     /translation="MDNLERNIQLIFSYLAKTFIRNGASIKDAEKEANEIIKANSNNL
                     WGVNGLAYQLGKINLEFFCMYFMQDTYLPKEDNAAAPIAKVHHELWEDIQESIIGDGS
                     QQLGRIYPRGTGKSAFGDLATTVWSHCYKHKTYTLICSDIGSTAEKFVKDIKNALLEN
                     EYIKKAFGVLLNDNDRKYICNSTQLELTNKTFIEAISSSSPMRGRKYNNNRPDLIILD
                     DYQSEENVRTEDAREKKFKRFSDDVKYAAQKPVIRNGKTIKRGTTFIALGTLQHKECF
                     YSRLKNLPTWKFKCEKGVLVDNVDELFNSGLWLKFKEILFDFKNTNHLEDAKEFYWEN
                     EKEMKFPILWPSFWDCLDMALSYYENPTSFKQEVQNDVNSIGEKWFKTVRTETREEIE
                     SHTFKKTMLLCDPASAGGSKHDYSAFLVGSESENGLLYGRLAELAKINARTDFDKYID
                     HMIYLLKVYSDITHIYIEKNTFNGADANQLEFKIKNDNVLSYRDIEIINEQQKKNKDD
                     KISTLIPVLSKGQMIFAEEDKEFIQQILDFTGQKYSLHDDAPDISSEFINRINNIKVS
                     ETVNIFDRKLLGF"
     gene            15876..16064
                     /locus_tag="ACGI59_000784"
     CDS             15876..16064
                     /locus_tag="ACGI59_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9738590.1"
                     /translation="MANRSKFEEVQKTLTKKGKKFNVGIGKDEKGYFAYTHRARSKSY
                     ISKQDIPIKVLKFIESTG"
     gene            16077..17354
                     /locus_tag="ACGI59_000785"
     CDS             16077..17354
                     /locus_tag="ACGI59_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA];
                     GO_process: GO:0099001 - symbiont genome ejection through
                     host cell envelope, long flexible tail mechanism [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFG9738591.1"
                     /translation="MELDLDLIKDIYDDWNSNKSEYDTMYKYYKGETDAISNYKMVTK
                     RSNNKINTNFLKKFINEEVAYSLANKITYTSRSGDEKIINDLEYYTCHWSKKHDSDLL
                     RYALLFGLCYELYYVKDDEIQVKIIKPTDGCHYENENGEIVCFIREFTKGFEDDTYVD
                     VYDKEYIYHFDSNFKEVESPTVNNIFDGNVPISICKRSEELGKNTIFNDIKGLQDAYE
                     TNLSDISNEISDFRNAYLTFSGCNIKEDDLPRMKELGILQVNGDGKIEWLIKDMNDTF
                     VQNTLSSIKENMYEITSHINHNEKMQSNTSSLAVIARLISTEWICSQNNDSIADTLFN
                     RYKLLCIWLNKKYGFDYDYKDIKAKFTPKIPRDDLVVANILSQLGDKLSTETGLSQLS
                     FIDNPHAEIEKAKKEQEIVSEGEILLDESKDYN"
     gene            17332..18093
                     /locus_tag="ACGI59_000786"
     CDS             17332..18093
                     /locus_tag="ACGI59_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HFG9738592.1"
                     /translation="MNQKIITKLTEDIYKSAENRTKPFYKYQRENRDKLLLEIAKILL
                     THDIINEYLNINNKDKKNLRSKLNKIINEYSNSYDEEVKDIETILSDSSKNKYSNLII
                     LLGVILKIKDVSNINNKVINKIVNNKVDGKHWSDRLWKNKKGIEESLKKEIKSFLNGK
                     TSVNKIEKAIRDKYSTGAGQTSRLIENEVARCQSEVNEYFFKVQGIIKVMYCADLDSR
                     TCSDCSMHNGVIFYVNEDRPSLPRHCHCRCQYIPV"
     gene            18152..18478
                     /locus_tag="ACGI59_000787"
     CDS             18152..18478
                     /locus_tag="ACGI59_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9738593.1"
                     /translation="MKVEILGTEYLVIEKLESEDVLLKERAGYCDHSVKEIVIEKINS
                     EEGSLKDLSVYKNEVARHEIIHAFLSESGLKSCSSWATNEEMIDFFAIQFPKIVKVMD
                     KIECLN"
     gene            18504..19106
                     /locus_tag="ACGI59_000788"
     CDS             18504..19106
                     /locus_tag="ACGI59_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HFG9738594.1"
                     /translation="MLKKEVLELLKEIEDDAEIDSLLQSTDLFKSASDKKLTIEEFRE
                     LINNDANFKAIIDNEKNKYHSEALENFKKKDMQTLISAEVLKRTGANETEEQKAIREL
                     RESLDKLKKEKQYAEKVSKYKDILVEKKIPTNLIEYLLSDDDDKTNANIEIFENSMKQ
                     YVQSKVDERIKDGSYVPPGKDSAGSLSEIRKQIKQGLNSL"
     gene            19135..20058
                     /locus_tag="ACGI59_000789"
     CDS             19135..20058
                     /locus_tag="ACGI59_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9738595.1"
                     /translation="MANVLQYVTLFQQELDMQVTVGSTTGWMEKNAGQVIYNGGREVK
                     VPKVTMDGLGDYDRSKGYTQGAVTLEYETMKMEMDRGRTFMLDSMDVNESNFVINASS
                     VMGLFQKQHVIPEIDAYRYSKIFSETAKVDERVKYSYEATVTTILSELKNQLASIQDL
                     SGDIPLIISMSSIIASILEQSSQLDDIEFGDGDIKRIVKSIDNVPIIKVPSKRMKTEY
                     VFRDGKTSGQTEGGFTEGAKALDINWLITPASAPIAVSKTDKPRIFTPDQNQTADAYQ
                     IDYRKYHDLWILDEYLKLCRASVRQAKAAQE"
     gene            20072..20344
                     /locus_tag="ACGI59_000790"
     CDS             20072..20344
                     /locus_tag="ACGI59_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HFG9738596.1"
                     /translation="MFALIKNNIIKVVEDENSRDKLISKGFKIYSEEVTKENIEKKEE
                     KVKSTSNDLFNLSKPELMEIAKGKNIKGYSTKTKEELIQLLDEYNA"
     gene            20337..20708
                     /locus_tag="ACGI59_000791"
     CDS             20337..20708
                     /locus_tag="ACGI59_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HFG9738597.1"
                     /translation="MLDNVKLVLGIENDRYDKLIILYINKISEMVLEYCSRQELSTAL
                     EGFVEEKVISILSSRFEELKKNSGLDNLEKSLKAITRGDTKIEYNALTLQTAENSISS
                     SSFLTDTDKKFLSPFCIVSMY"
     gene            20720..21067
                     /locus_tag="ACGI59_000792"
     CDS             20720..21067
                     /locus_tag="ACGI59_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9738598.1"
                     /translation="MTEADILEMTYLDKMTIIRKLKGWNEETCSNDFSNVVIAENIPC
                     AISRKEEAIVNGDIGSIVVNRKLFCRPEVDIKIGDTINTTYNHGEEAVFKVSKANIYP
                     SHMEIPITELERI"
     gene            21067..21423
                     /locus_tag="ACGI59_000793"
     CDS             21067..21423
                     /locus_tag="ACGI59_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HFG9738599.1"
                     /translation="MGIDNLGVFEALALDLGLNASNFNRKIKKFTKETGQELAENIRD
                     ETPVKTEKLKKGWEEKQPEYDTYIVKNEVDYATLVNNGHSTKNGGIVPGSFMVEKGIE
                     RTKENMTSAKANLFDW"
     gene            21432..21881
                     /locus_tag="ACGI59_000794"
     CDS             21432..21881
                     /locus_tag="ACGI59_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6838 family protein"
                     /protein_id="HFG9738600.1"
                     /translation="MLTNRDIIIAVNKRIENEFRYIDIFINEDNIQGFEEACFFVQLL
                     PVSNNIANKVLDTKKIFIDIEYYQKARKNKLNLYDIQSRLEKIFNRNIRVKDRSLIIE
                     NISPSIAKDPVGYKLTFLMTVSYFEEVYFENETFEIMESVDLEIGSD"
     gene            21882..22937
                     /locus_tag="ACGI59_000795"
     CDS             21882..22937
                     /locus_tag="ACGI59_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HFG9738601.1"
                     /translation="MGLPEINISFIQAGKTFVKRSGGIVALILKDTKNIGLVEIKEIE
                     DIPENYSAANLDYIKMAMKGNVYPPNKVLVYTLDTDESIDDALDFLETCEFNYLCMPD
                     ETEQDLPKIKTWIKKMREDNKIKVKAITASNSADYEGIINFTATDIEVEGKKYTSNEF
                     LPRIAGFIAGTPSTQSVTYAEIPEVTNIPKLTRAEANTRINNGELILIKESGAIVIAR
                     GVTSFTTITDSKGDIFKKIKLVDTLDQIHNDIKKIIVKNYIGKTQNTYDNKCLLIVAI
                     QLYLQELEKDGLIDEGSTVEINLDAQRAWLKKQNIDISNMEEQQIKEYNTDTLVFLKA
                     RIKLIDAMEDVYLDISM"
     gene            22953..23393
                     /locus_tag="ACGI59_000796"
     CDS             22953..23393
                     /locus_tag="ACGI59_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFG9738602.1"
                     /translation="MKAKIDAKRIISGTFGECWLDNKQLTSTKGMEAKVDIDKEEIKL
                     CGQIMTTYKMIGATGKGSITLYKISTLFGEYVEKMLNEGKEFKVTIISKLQDPDSYGS
                     ERIALYGCMFDDLTLADWEAAKVGEIEVPFTFEGFEYLDKIKEK"
     gene            23408..23824
                     /locus_tag="ACGI59_000797"
     CDS             23408..23824
                     /locus_tag="ACGI59_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFG9738603.1"
                     /translation="MNTVEKLLNMDAGKLKMPSSAFELYCKKLDDTLEIECKAIDPER
                     FDEIRMNSMDVNSGDVENINIYELKVNTILESCSIFSNMELVKKFSAITPKELIKKLL
                     LSGEIDKLYEEVNKVNGIDAEGDKKRQKERAKDIKN"
     gene            23851..24000
                     /locus_tag="ACGI59_000798"
     CDS             23851..24000
                     /locus_tag="ACGI59_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9738604.1"
                     /translation="MYLLFKYKGIMPSVSYHILRGEKEIVSVFMDQELDERKEEIKSG
                     VGRLF"
     gene            24004..27393
                     /locus_tag="ACGI59_000799"
     CDS             24004..27393
                     /locus_tag="ACGI59_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFG9738605.1"
                     /translation="MSSNTKVLQAIIKMRDEASSTLKRVAANTRALHDSSSQASRALN
                     NVQSNLKSVGTSALKAGGLITAGVGGAFAAATKVGTEFESAMSQVKATFGNSLKPGDF
                     EALSKAARDAGKATTKTAADSANALQYMGLAGWDVQKSMHALMPVLNLSEAASMDLGL
                     ASDLVTDSMSSMCLEVDKVSKSGKVLAGAGLSEYLDKVANASTKSNTSIQQLMEAFNV
                     AGGTFSNLKVPLSEATAIMGILANRGVKGTDAGHALNSIMVNLTTGAGQAGKAMKKLG
                     VSAFDNQGKFKGMSNVLKEVANKTKGMTDEQKNYYLSAIGGKTQLKTLQKLLDGVGNE
                     YDSLKKKIDNSKGALKTMSDVMKDNTKGGLATLKSALEETGITIYYQLKPAIASVVDN
                     LTKMTVWFNNLSEPIKRVITNVMLSVGAFGVLLLTIGAVSLIVSKAIGIFGFFVPIIL
                     RAKVAFRAFVIGMKPLSAVFLAVMGPVGWTITAVIALSVAFTIAYAKSEVFRNKVNSL
                     VKSFTEFAKPIVNFCMPALKQLGEKFKELLSAFGPLFASILNLGSVIMKVLGPVLIGV
                     IGFHIAKLVLILSTLATAFKAVVDTITNVLNGLASILNGVFDLIIGIVTGDSKKMMDG
                     VKQIFDGGTKIIKSIWKGFIDFVTAPIQAIADIADSLFKDKAKWLTDKWRDLRTFLEN
                     PIEATAKISTKTNVNIPGSKAADAAGKSTPNTGGLKQTVANLKGSFSDFKTFIKPMTD
                     SISQGVESGKTKFTELQNKILNDVKPALKVLEDSGVNFKDKFKESITSIKDKFNELKN
                     ILAGNIKTNIDNLVKAFEPLKPHLDNLKDCFDKVKKAITDFFTPAKQVGDTVNKVSQP
                     MDSVKKSTDGIKLSFQSLTQALAPVKAWFQGFMPFLSQIGQQLLPSIGIAIAGVVGGI
                     ILAFTWVLNTLTAIITTVSGIINGIMSFISGIINVIMGLITGNADQTMDGLKQIFDSG
                     VGVIKSIWNGLVDLVSGVLEPVIDLADSLFKDKVKGVKEAWNKLKDFFKHPIKGTIEI
                     FKKGPSLQIGKGADAAGKSPRKAFGINRVPRNDYLIRAHEGEKLLTKQEANQYKRNSS
                     NGSLFLDKLADTIIVREEADIDKIVRKINKKISLAKIGGVL"
     gene            27440..27916
                     /locus_tag="ACGI59_000800"
     CDS             27440..27916
                     /locus_tag="ACGI59_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HFG9738606.1"
                     /translation="MNKKILVLTISIILSLSLVGCSTNNSKNRDSKSKQNTEEVKNHK
                     IGEVVDLDGAKVSVKNIRKLETNSNDYAVEIEITNTGDNDLQSDYLENYILKSSDNTT
                     GEAINNQELNGELMFDTIRPGDTLKGEIAFELTKDATPKTLEIAYENVGKYTVFDL"
     gene            27974..28609
                     /locus_tag="ACGI59_000801"
     CDS             27974..28609
                     /locus_tag="ACGI59_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFG9738607.1"
                     /translation="MEIWLRQANNTFRFPVIPPSFEINGSATINTSNILSVGDIAVFG
                     GLGLKTIELSSFFPNQEYSFCNYNGFPKPYDCVNLIESWMKEGYILRFIITETNINFE
                     CIIADFNYREQDYSRDIYFTLSLKEYRRIQISKVNINNDEKLSSEKNVPLTKGFDTKQ
                     KTHKVVEGDTLFKIAKKYYSNGDLWEKIYKANEDEIKDPSVIKNGWVLIIP"
     gene            28850..30367
                     /locus_tag="ACGI59_000802"
     CDS             28850..30367
                     /locus_tag="ACGI59_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFG9738608.1"
                     /translation="MIIDRSKDSSNNNIDFTAKDMGFLLLQSEVSYNFKDKLVEDIAK
                     QVFVDNKFPLGNLPKTNVKYTKMFIGVTGYDTIMSVYTEASKTTKKKYMIESNLDKFN
                     VIEKGVVTLNITFEEGFNLINTNFSESIENVKNKVLVVDQYGNKISEKINDSIFKDVG
                     VIMQKVIQQQENSTIDIDGEFKGIEKTCSLKGYGDITCVTGRGVKVKDSYTKLIGLFY
                     IDTDKHTWQNGDYQIELELNFENIMDEKSAGQDEQKEDGSEYTGGTEYPAEFTAYCPR
                     KEEGGNTDCRGKKLDPSKKTCAAPMVGAYEKSYYTKDFLNKHPLMRYGDEIQLVTGVS
                     SRDGTYKVNDNGPAIIIEKDGTYHIDILFGNVEEANNFGRRKGKIIIGGYSGNMTEKA
                     KTVISEARKHLGKPYKWGGNGPSSFDCSGLMVYCFKKVNVNLPRTSSQQSKVGKKVEK
                     NNLQAGDLVFFHNPVSHVGLYVGNGEFLHAPQTGDVVKISKLSNRKDFNTARRVL"
     gene            30381..30713
                     /locus_tag="ACGI59_000803"
     CDS             30381..30713
                     /locus_tag="ACGI59_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2577 family protein"
                     /protein_id="HFG9738609.1"
                     /translation="MADPINEFIGIIRQEGKFHNEPSFFIGKIKSKLPDLKIEINNII
                     LEKEDILIDSWMLDRQIELFDTETSQEHKHEIKNPFIDTFESGNTVIMFKIGEKFAVV
                     SKLVSLDE"
     gene            30706..31140
                     /locus_tag="ACGI59_000804"
     CDS             30706..31140
                     /locus_tag="ACGI59_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HFG9738610.1"
                     /translation="MNNTIFPFMGVPEDYISPDNEELPIFKEFAWDFNKDEKIIENGD
                     FKIVERNEAIKVWIYKTIKTDKYVHLIYDWSYGTDIKNLIGQKYTKGLTESEAKRYIQ
                     EALLTNPYILEVNITNAEFEDDNLSISLNVKTIYGEEEITFV"
     gene            31133..32185
                     /locus_tag="ACGI59_000805"
     CDS             31133..32185
                     /locus_tag="ACGI59_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFG9738611.1"
                     /translation="MYNGQSFSTLNNRTLNNINLPLYKGQGSSLYNIVSPINSELAQL
                     YIELSYIHKRVFIQDNFDDFLDRRVNEFGVYRKLGTEAIGEVTFEGKIGTQIPNGTII
                     SHSDLLFVIIKDITIDENSKLNVSPIQALEIGIKYNLSANTEFKLIEEITGVTKIYNE
                     LDLKGGTEIETDEELKERFYKIQKNQATSGNKAHYQSWALEVEGVYNAKVIPRWDGPG
                     TIKILIYGQNNQSVDNEVLQRCTEHIEEEKPIGPTVTIVTPSTFDINISATLTLENGY
                     DIESIKVVFLDIINSYLIENSREIVYIKVMSLLASIEGIHDIKNLLVNGDVKNIIVDE
                     EKVPAVSSAIFDIEVS"
     gene            32187..32795
                     /locus_tag="ACGI59_000806"
     CDS             32187..32795
                     /locus_tag="ACGI59_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015981513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative phage tail protein"
                     /protein_id="HFG9738612.1"
                     /translation="MKLIDYLPSFANNEIDIQIQEALENELLTLIDEKDDLLEQFFID
                     TATWGLDDWENLLSIKVNYKLDFDTRRSNIKAKMRGKGTTTIEVIKAISEAYTKTNVD
                     VEVFSNLFSFTLSFITNNCSYNTILELDKKIEEIKPAHLEHKFERILFNKNELYTGAA
                     ISTGETVTIYPYVPRNLESFGEIAICSGNDRALEKVTLYPKK"
     gene            32812..33594
                     /locus_tag="ACGI59_000807"
     CDS             32812..33594
                     /locus_tag="ACGI59_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFG9738613.1"
                     /translation="MTEQQYFTLVTDIGKAAIANASITGEKVDFAKIKVGDGGGSSYT
                     PNESQTALKNVVWESTLEHAQGDKDNPNWVVIQKFIPGDVGGFEIREVGLFDSKDQLL
                     AISSYPTTYKPTADSGTVKELLIKVILVVSNVANINLKVDPTVILATLKDIQELDTKI
                     DTTKTELTSNIETAKTELNTKIGDTTQLTTTDKTNIVSALNEVKTSVDSIETTADKTS
                     IKDTDNLFESDNVEGALKEVMQEVKGNRSSIISTINSNLIPM"
     gene            33611..35056
                     /locus_tag="ACGI59_000808"
     CDS             33611..35056
                     /locus_tag="ACGI59_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9738614.1"
                     /translation="MKIWKYNRTVKKRRGVYTELDILAQRYEFEADGKLYTAYFHLYT
                     NKNIDIFRDKDLYKESYMMSYRVGNVTTDIDDVVQQTTTVRFIPDPVIDIVIDNISRG
                     CEINYRIIDNEPSVKFIVTEKLNGTIIETKTNSSDNNYKLKLTDEQIVPLAFNSQNSI
                     VIEISTEDGGLVTSKTVTFTRTNNKPTILVNSYSSNSAKFTASDLDNNLSKIEWYLDD
                     VLKETITTDLTAEKTINYEFTDNAIHTLKIVATDAENATVEKVLSISKEIMPLQSDAS
                     LSDISTKLIEIGEGFRNGKTSIINTLALKNIEASLNNTLVELSEKIKTSFDSSDASVQ
                     DLQNRITELNNQLSRRKKWATGRYTFTREEDTINFRMVDGDSLSAVKKLIIPINLDFV
                     PSVIIIDKMFLGSYVPAQDVQFNCVSNLADFTLGRRFYNKQWICTSSIKLDSVTQNSF
                     TLSLQKVVPFDKVSSTIFSIINTGFAWRAYE"
     gene            35080..35427
                     /locus_tag="ACGI59_000809"
     CDS             35080..35427
                     /locus_tag="ACGI59_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9738615.1"
                     /translation="MNRNNRIIYDQTGKIWLQTGEAIGDIQELSEITELNFIDVEFGS
                     IDYSKQYIESINPVTKELNIKNIEVVLTDEQKRLEVLEKELNMLKEENKNRDSEIVNT
                     AFEVGNIKLNNNL"
     gene            35439..35609
                     /locus_tag="ACGI59_000810"
     CDS             35439..35609
                     /locus_tag="ACGI59_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9738616.1"
                     /translation="MYNLLKLMIEQKNYSTKEDLQHKMDVFYAVNRITEEQYLELTGL
                     LNKEETPVEPTI"
     gene            35639..36208
                     /locus_tag="ACGI59_000811"
     CDS             35639..36208
                     /locus_tag="ACGI59_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9738617.1"
                     /translation="MTFKELVSKVRNLVLEAKNVTIEDTENKFTSENVEGALKECIDR
                     ADLAFQSADSGKELIATAIGSPATSEQTFQEYANYIAGFKNTISQLNSKYKYTSGTFT
                     YYTGAYPNGLNIGFRPDIISYTAINAVEEKYYCVYTPLFYLFYNYTTGEKYLDVNHNR
                     YGITFTDNGWIPRNPVNNGVYSYIAAKCR"
     gene            36267..36695
                     /locus_tag="ACGI59_000812"
     CDS             36267..36695
                     /locus_tag="ACGI59_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFG9738618.1"
                     /translation="MNVTIVFLATNIFIKLVILAIAFDTLLGCLRAIKTHQFNSSFGI
                     NGGIRKVAMIACIFFLAVVDILTKFNFLFMLPQDWVDFLRLNHLGISEFFSILFILYE
                     SVSILKNMYLCGLPVPKRLKEKIGSLLDTMTDELNVKGGK"
     gene            36699..37529
                     /locus_tag="ACGI59_000813"
     CDS             36699..37529
                     /locus_tag="ACGI59_000813"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003415961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFG9738619.1"
                     /translation="MKICITVGHSILKSGACTSADGVVNEYQYNKSLAPVLADIFRKE
                     GHKVDVIICPEKQFKTKSEEKSYKIPRVNAGGYDLLIELHLNASDRQGKGSEVLYYSN
                     KGLEYATRICKKLGTVFKNRGAKLDKSLYILNSSKPTAILIESFFCDNKEDYEKAKKL
                     GYEGMAKLIVEGVLNKSIESNEVKQMYKHTIVYSGDDRVSADILGLYYKRAKESYLVI
                     DIKDYKPHRTQNLYVIGGVTCNKMKEMSKTTGEKFTQLYGNDVWSTMDKVIEFVKEKL
                     "
     gene            37601..38707
                     /locus_tag="ACGI59_000814"
     CDS             37601..38707
                     /locus_tag="ACGI59_000814"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFG9738620.1"
                     /translation="MLTFLDLFAGIGGFRLAMERAGHKCVGHCEYDKFANLSYKAMHN
                     PKEDEWFEKDIREIRAENIPKADVWCFGFPCQDISIAGNKLGFKGNRSSLFFSVTKLI
                     RELKEEDRPKYLLIENVKNLLSVNRGFDFAKLLIELDEIGYNAEWQNLNSKNFRVPQN
                     RDRIYIVGHLRRRSTREVFPITGTSRKTTLEQLNNPNHSSNRIYNSNGLSKCLLSKGG
                     NNTGLYRVGNINPSGNGAGGQVYSSEGFSPTLTTGKGESPKILIKENTKKGYIKASIG
                     DGIRLDHIEGKTGRGRVQHKKSCTLTTASNAGVIGKKFRVRRLTPKECFRLQGFPDDY
                     YDRAASVCSETQLYKQAGNSVTVDVVEEIAKKLK"
     gene            38914..39180
                     /locus_tag="ACGI59_000815"
     CDS             38914..39180
                     /locus_tag="ACGI59_000815"
                     /inference="COORDINATES: protein motif:HMM:NF015898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HFG9738621.1"
                     /translation="MSKKEIIRICNEKHNWRKSATKTLLKRLVKKRVLLKRIKIFHIY
                     YIPIVTEKEYFKYKINDLEPDYLDEYFIRLMSTVHKFKNLDEED"
     gene            complement(39191..39706)
                     /locus_tag="ACGI59_000816"
     CDS             complement(39191..39706)
                     /locus_tag="ACGI59_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9738622.1"
                     /translation="MTWSDLKEKNINEVVDCINNKLSEFESLKKVGDELGANESTIRK
                     WLNKKGYKRTGNKFVEDNKSYDDKYHIDSISSKIDDDIKYLSSEIDNIKNVLEWFKTK
                     DDSYHICVIKENDISIDLPDQSIKRTTVRINNTVWEMFNKFADENKHYDKHDLLSQLL
                     LESLLKYVKSR"
     gene            complement(39789..40694)
                     /locus_tag="ACGI59_000817"
     CDS             complement(39789..40694)
                     /locus_tag="ACGI59_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFG9738623.1"
                     /translation="MSKLGIDIGNYAVKTSTDDIFESKVTEVKNFGSDSDSIKIGNKT
                     YYLGEGDEEINIVKYEKENFLPLLLGAICRNTDDEIVDLGLGLPVKQFAGLRKNLIEK
                     LQGKEYHVEFTRGNETTKRDITIRSIQTFPEGVTGYLYYAKDIVDQIAGRDVVLVDIG
                     GKTTDIALVQGNKATDPYSINVGTINIYDAIKKSLEMDERFLGKVEIKREKIQDYINK
                     GFYLNGEKQDIKKNIDASISLFKEIYNELKLNYPVSTAAVVVMGGGAKLLGEAFKKNI
                     PGIIVMSDVDKHVFANAKGYKKMMK"
     gene            complement(40714..40917)
                     /locus_tag="ACGI59_000818"
     CDS             complement(40714..40917)
                     /locus_tag="ACGI59_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9738624.1"
                     /translation="MSRIVLTFKNNDKEKAIEKFLDEKLSATAYLKELVWEKMQEKKD
                     NAVVEQKKEIEDPANNFDFGSLE"
     gene            complement(41166..41624)
                     /locus_tag="ACGI59_000819"
     CDS             complement(41166..41624)
                     /locus_tag="ACGI59_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9738625.1"
                     /translation="MKKTIALLLTLCAVMIPFTAFADGNDYEGTGNSQTVELIGQPNS
                     DSPSTKSVVVPGGTANLTAMSGGQLSWRVTSTLGNILGFEGNIDVYKAGKIVSSQYVG
                     FRLAGTTKTGVVTFKGLKKGSYIAKFKGKGVCTGGIILFSSPAEASFTIR"
     gene            complement(41659..41961)
                     /locus_tag="ACGI59_000820"
     CDS             complement(41659..41961)
                     /locus_tag="ACGI59_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9738626.1"
                     /translation="MEVNLHTGKDEFFKSHEVAKNYIGGLYKLIDVYKFSMESVSVLT
                     RIDLDKLNNFYAGEASLNYDELCLIESVIITPLVGAFETAEHNYNMIIKARKERKL"
     gene            complement(42349..42501)
                     /locus_tag="ACGI59_000821"
     CDS             complement(42349..42501)
                     /locus_tag="ACGI59_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9738627.1"
                     /translation="MDNFLFNVLASLTASVVVYLISELFKKAKSHSRAKSDLQVEFKF
                     IFKFKK"
     gene            complement(42621..43355)
                     /locus_tag="ACGI59_000822"
     CDS             complement(42621..43355)
                     /locus_tag="ACGI59_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFG9738628.1"
                     /translation="MYYVYKYINLDTEECLYVGKTENICDRHASHLSNKKENWCNKNL
                     RFEYIELDNKYTMDFYEIYLINKLEPKFNITGKGEMDIAKTSFSYNGQWVIYSERDLM
                     SSLASKRCGINYEVNAKMLGIMRKLKKISSNEEIFIGNENIRIKYYFETVLEGINLEP
                     CILSVAYKTLKESTVGTVISVKREYLSENVCLIIDSIFLNEIMKDPLMSKSDIKDLNS
                     QVNDILKIIGVDCEWNKLSDYCSANS"
     gene            complement(43375..44562)
                     /locus_tag="ACGI59_000823"
     CDS             complement(43375..44562)
                     /locus_tag="ACGI59_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFG9738629.1"
                     /translation="MEKKEILMQPNNLIKSKYDFTNVENKLFYKILFNAQKQQNSSYV
                     TTISKEELKVFMKNNNDYEHKNIKEILNMFQQSVLEFDYIEETTGKLKTFGSGLINTY
                     ELDHTDQIYTIMMHEVLYNHITDFVKMQKKKNGYTAINLSVLFNFRGAYTQRLYTLFR
                     LWSRENKEVEIKYKLDELRFYLKLKDNVYPEYKYFKQNVLKRAMNEINKKGNMVVSIK
                     EEKRKNRKIDEIIFSVIDYEPRKYFDKDILVEDYIPKKKQDNEVSFSFYIPNENIFTT
                     GTIKLFKEDFKEFDFKKDVYLNAFNKSIAIAFERDNTDIINTKNYDFFKSTLSNKISE
                     VIIHQKDDLKFKEELDKYWDDEDVKKEEREVDYSKDPVRLETVRRNLFDQGLVNEEGY
                     YYK"
     gene            44915..45604
                     /locus_tag="ACGI59_000824"
     CDS             44915..45604
                     /locus_tag="ACGI59_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFG9738630.1"
                     /translation="MLENRKQIQKEIYLPIATYCLKDKQYYIKEYGALLIKSAENSDI
                     FNTHKNRILYKNKLDIQEIANELNVNKATIQRNIKKLEKLDFKILKIENTLNGIVYCL
                     PSENNIDLNKFALINYEMLKKMVNKFKSNTIKVYFLLKTITTETNFKPATNSFIAENI
                     GLSSKSKNNLDIITSSVKTLEENKYIETKKVNVYEYDKEKLREVPKIRKVYRICNFDE
                     WKKEIDKNKVY"
     gene            complement(45753..46136)
                     /locus_tag="ACGI59_000825"
     CDS             complement(45753..46136)
                     /locus_tag="ACGI59_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HFG9738631.1"
                     /translation="MKIKKIPESELLVMMFIWDSEDKEVASTEILKTLGEKYEWKKST
                     MLTFLRRLVGRGFLEVVKKDRFTYYKALIEKEEYLKVETKSFFSFFHKNSFESFISAL
                     HDDEEVSENKLKSLEKFIEDWEEDK"
BASE COUNT        18578 a         5376 c         7711 g        14583 t
ORIGIN      
        1 aaaaaaaaat taagcaggag taaaaaaaat aaaatattat caaaatttat caatataaaa
       61 agaaatataa gtataaattt ttctctttgt atattgattg tatacaattc tatttttgta
      121 aaattaaatt ttaaaaaata atcaaaaatg gtaaattttg acatttgctt aatgtcaaat
      181 taggtgttac aattgtagca taaagaagat atatatcgaa ataatacata taaaactacg
      241 tacgtaaaat atttttaaaa ataatcaaaa atcttaattt tcaatattga atacaattaa
      301 attttcaaac tcatctaggg catgacatac aaaagcaaaa aacattaatt tagtatctga
      361 taaaaattta aaaattgtaa aagggggaag tctgttattt ttcagaagca atgtaattgt
      421 aaaaatataa atcagaatat tttgaaaata tgggggataa acttatgatg gatttagaaa
      481 aggaaaaaca tgaaataatt gaaattataa aaaaattaaa tattgataaa gataaaagga
      541 atttattgat gataaaaaat tatatccttg gacttttaaa aaaatagaag aatagcaatt
      601 gctattcttc tatttctagt aaggaatcta tcattttatc aaatacagtc agatattttt
      661 tattcaattt gaacatttta gttataattt ctctttctac ttcattttcc attttactta
      721 gttcaccaac aagatgggca aattgctcat ctaattcttt gataaaaaac atctctccaa
      781 ctccatgctt aagccattct tcagatatgc tccaagtctt gcaaatgtcg ttcaaagttc
      841 tttcgcttgc atttctttta ccattttcaa ttaaagatat tgtgtttctt tttaaattta
      901 gtttattagc aaactcttct tggcttaaat tgagatttaa tcttagttct cttattcttt
      961 tgttttctgg tttcatttca tctatcatta ttatccctcc atatgaaata ttatatttta
     1021 aaagtgctta caagtcaaca aaaataaaag aaaaaggtat tacaaaatag ttgacttgta
     1081 agcagacatg taatataatt gtattacaca atcaacaaaa gcgaggtgat tgaaaatgac
     1141 tgatataaat aaaattagag aagaaatata tagaaaattg gagttacttg gccctgcaaa
     1201 actaaaatta gcattgacta aagtagatat attaaaagaa atacaagaca ttgaagatga
     1261 aaataatata caaaattaaa ggggtgaaaa gattgaaaat ctcagatttt aataaaaatc
     1321 aaataggatc agcagttcaa ttacaagata taaggtttga tacaaaagct gattgcatca
     1381 ttacgaaagt agaaaagaat gaaatactag tcatgtatta tgaaaaagaa acagaggaaa
     1441 tagcatataa aactttgact aaagaagatc taatacttga tgactataaa cttaagttat
     1501 tatcttaaag taatttcaga agggaggtga taactttgga tcttttgaag gatgcaagaa
     1561 aaaaagagtg gttttggctg gaaaatgatt tgattgatag agaagatttg agcatctatg
     1621 aaaaaatgat ttacattgtc ttagctagat atagtaacga agaaagttct tgtttcccaa
     1681 gttataaaac aatatcagaa aaagcaggtt gcagtataag gcaagtatct aatattttga
     1741 atgaattaga gaataaggaa ctaataataa gagaaaatcg taaccacgaa ggcaaaaaag
     1801 agaaaaacag taacttatat ttcttaatat cttcaaaagc aaaggtacag aatgaagtgc
     1861 ctaacgttag gcatgaggtg cctaacccta tggcaccagg tgccttacat gttaggcatg
     1921 aggtgcctag taaaaagact tataataaaa agacttatat aaaaagtaat accactacac
     1981 aaaaagaatc taaaactgat tatttagacc tatcattctt ggacttagat atagaaaaag
     2041 taaaactaac taaagatgaa tatgacaaac ttataagcaa gtttgggaag aaatacatac
     2101 acgataaaat tgttagttta gaaaactata tcataaatgg taaagggagt aggtataaga
     2161 gccactatag ggctttgctg acttggggga atggagatgc tagtaaaggt atagtaccag
     2221 aagttacaaa gactaagaat cctcttagtg gatttaaaga actttaaaaa ctaaatagta
     2281 aggaggtaga taggatgaat gaaaaagaag agactgttta tttgaatagt tctaatgtga
     2341 agttattctt aggcgttagg gatattaagg aatttgagga attgatagaa aatgtcaatg
     2401 agcgaatttg tcaactccaa gaatcgatta ataaacttgc ccaatttaat attgagttcg
     2461 atattaagtc agatatttag ttaagtataa attaggaggt tgcctatgaa agatgaaaag
     2521 ttagaactta ctgaaaaaga tttacactgt attgcaagac atttacaaaa tgaagttatg
     2581 gagatggtgt ttagagggaa tatagaagcc cctacatcat gcgaagtttg cgattacttg
     2641 caagaatgca aggactattt cacccatata gacactttta taaaattaag tgaaatgaca
     2701 ggtgtagata tttttactaa ataaatttag taaattaaaa ttttagtatt gtaattgacg
     2761 tgctttttta aaaagaggac agttgctatt agcagtcatt atttgaattt tgcaaatgta
     2821 atttttatta cattcgcatt ctaaaagttt tgatccagtg aatccaggaa aagttttata
     2881 ttcaccagaa aattcaactt tatcgtcaat aagagagcat ttttctctta aggttttaat
     2941 tttgcacata ttcatcacca ccctataatt aatttgaaca tactgtcaat atgctccgat
     3001 taattataac atgcaaatta tcatgatgta gaattaaagc ataggtcaat taaatttagt
     3061 tataaaatca atgaaacaga taaataatta aatgttaaat aaattagggg gttgttcatg
     3121 aaagaggaaa agttagaact tactgaaaaa gaattgcatt gtattgcaag acatttgcaa
     3181 aatgaagtta tggagatggt gtttagaggg aatatagaag cccccacatc atgtgaagtt
     3241 tgcgattacc tgcaagaatg tgagggagat tttacctgta tgtatcattc ttttactaaa
     3301 ttaagcaaaa taacaggtgt aaaagtgact gcatttaagt attgttttcc agatgaaaat
     3361 cccaaagcta ctatctaaga agttttttta gaataggaca ttcttcatta gaagaagagt
     3421 cgcaaatatg acttccatca catctagttg acattaactt agatccaaaa gtacctggag
     3481 gagctgaata ttcgcaagta agagaaatgg tttcgtttat tacaggacat tctttgctta
     3541 taactcgata ttggctcata ctctcaccac ctttctaatt aatttaaaca tattggaagt
     3601 atgtttaaat taattatagc atgtaaatta tcatggtgta gaatagtttc aaatcaaaag
     3661 aagcaactaa atagttaaag acaggaggta tgctaagtgg aaagaaaaaa acatgaattt
     3721 acagatcaag atatacaaag acttattgaa ggattaagaa ctgatccaaa aataagagaa
     3781 atgatattta aaagtaaagg ttgtgaaact agaacaagta caagaagtaa atgcgaacaa
     3841 tttgttgaag aaaaacttgc ttatagaagt tatggatttg aaactccaaa atactatgca
     3901 aaacttattg ccgacataat tgatatttta gtttacaaaa atgttccaag agatcgtatt
     3961 tgtggggtat tgtatgacgt cgaaagggtg attcctttag ttgtgacatt ttaataataa
     4021 gctgggggtg attaaatgaa ttactcttta gaagcagagc aaaacattct aggaagtttt
     4081 cttataaatg actctatagg ttacaaaata agggaattaa aagaaaatga tttctacttt
     4141 gagtcacata aaattatttt atcttgcatg aagaaaataa ttgactcaaa taaaccacta
     4201 gatcttttgc ttttaaaaaa tgaattagaa aaaatagata gattagtaga tattggaggc
     4261 gttagttaca ttaccagttt aacaactata gttataacta cttcaaacat agatcactat
     4321 ataaaaatca taaaagagaa gttacttaaa agacaaattt cagagttagc aaatgaatta
     4381 attaataatt caaattcaga tacaagtata gatgaattga ttatagatat aaacgattta
     4441 aaagcacttg tgacgacgag cagtagtgta aatgataatt acattgacgc atcaaaaata
     4501 aaacgtgaaa aaggcgtaca caagtccata gacacgggtt ttaataaact aaacaatatg
     4561 ctagatggat ttagatatgg aactcttaca attttaactg gtaaacctgc atctggtaaa
     4621 tcaactatag ttaaccagtt tatagcacaa gcaattacga atggagagaa agcatttttg
     4681 tattctggag aattaccctc tttcatgtct atggattggt ttagaaaaac tgtagctaat
     4741 gattatcaca taaaagaata taaaagtgtc tatggaggaa catacacaga tattccagat
     4801 tacgccgttg agcttatatc agactggata gaagataaat tttttctcta tgatgaagat
     4861 gcaataagtg atgaagtaaa tctactaaat accatagagc atttatattt aaaaaaaggg
     4921 gttaggttct ttgttcttga caatctaatg acaataaaaa ctggcaataa ggcagataaa
     4981 tatgagagac aagagcagat agtaagcaac ttaaaaaatc tcgctaaaaa atataactta
     5041 gtaatagttt tagttgcaca tcctcgtaaa aatatgggtg acatgaagcc aactatgtat
     5101 gatgtgtctg gagcaagtga gattgttaat tatgcagatt atattttatc aacttacaga
     5161 gttgttgatg aagaagaaga gacagacgac acttatttat taattttaaa aaatagaata
     5221 acaggaaaac agaatataag ttttaaaatg aatttttcag aaagaagaaa aagactttac
     5281 acgactagtg aggagttaaa cagagattac aaatatgatg taaataaaca atatgttcaa
     5341 gtagaaattc cagaagatgt attttaaaaa aagtataaaa aataggaaat tttattaagt
     5401 aatgatttta taagaaaaac caatattgaa taatttaaaa attcaatatt gaaaaaaatc
     5461 atttttcaat attgaaaaac ggattttgaa aaagtattga atttcaatac taaaaaaagt
     5521 ttgaaaatat agcatttaca aggtttatat aaattaattt cctgacaatg tacacacagg
     5581 tttagatatg ctagcaaaaa aacttaaaag tagtgtaagg ataaaaaggc tatataaatt
     5641 tataaaaaga tttaaaagtg ttattaaatt agagctaaag tttattaatt aatttcctcg
     5701 tagaaaaata ttattttaaa taaataaagg agtgatttta catgatagca ttagaaaaat
     5761 ttgcaggtgg agttctaaaa gaaaagttta atacagagtt acaaaaagtt ttagataaca
     5821 ttgcagatcc aaatacagat tttaaaaaaa caagaaaaat tagtttagag atagtgttta
     5881 aagcaaatga agatagagat cttgctgaag tagatataaa atccaaagca actatagtag
     5941 aagcaaaggc tacaacaact aaagttctga taggaaaaga tttagagaca ggaagagttg
     6001 aagcgtctga atttaaaaat caagtagcag ggcagttggt catggatatt ccagatagtg
     6061 aagaaactaa tgaaacagaa gaaaatagtg ctggtgtaat tgatttcaga aatgcaagtt
     6121 caaagtagtt taagaaagta agtaaaatat aaatttaaat aaaaaaggag aataaaaaaa
     6181 atgataagaa atgcaatgga atatttagtt aagttaggaa aaagagaaac agtatgtgta
     6241 gatgggttta agtacacaac agatgcatta gaaataataa aagaaccaaa agctaatgaa
     6301 ttagaaataa ctacattgag tggtttagta gattatataa aaagtggcat agatcataag
     6361 gcagatgagt tgttattaat acaagttcta actccaagaa aagtgctttt aaaatctcct
     6421 ttgagaaaaa atagagatag agagacatat attgaatgtg ttgcactact accagagaaa
     6481 agatttgaca gacctttaga cttagatagt tttaacataa tgctacaatc agcatttata
     6541 aagaataatg atagagatac acttttaaaa attgcaggaa atatccaaga atctacagta
     6601 aaaaatgtgg gagatgatgg agtttctcag tctgtaacaa taaaaacagg cgtagctagt
     6661 gtatcagaag caatagtccc aaacagagta aagttaattc cttatcgcac tttccaagag
     6721 atagagcaac cagaaagtga gtttattttc agaatgagtt ctggaccagc agcaggatta
     6781 tatgaagcag atggtggagc atggaaaaat caagcaatgt taaacattaa agcgtacttg
     6841 caagaagaat taaaagattt taaaaatgta aatataattg cttaattgaa aatctaagag
     6901 ggcttacaat aagtcctctt agtaaaaaag gaggggatta gatgaaaatg atttctcata
     6961 tgaacatttt agaaagagca gaatttacag ataaaatagc taatgcaatc atgtctgcaa
     7021 ataaagaaat tgaaagaggc tgtgccttta atgaagcagt tgagattgta aaacaaatgg
     7081 aatatagaga aaataaatac aatgaaggag atattgcaat atgaataatg tagttttagt
     7141 tggaagatta gcaagagatc cagaattaag atatatacca gagtatggta ctccagttgc
     7201 tacttttgca ttggcagttg ataggggtta tacaaaaaag gatggaacta gagaagtcga
     7261 ttttatacca attgaagtta tgggaggatc cgcagaattt tgtgctaatt atctcgcaaa
     7321 aggaagaatg gtttcaattc aaggccaaat aaggatagaa aaatatgaaa aagatggaga
     7381 gaaaaaaact ttcacaaaag taagaacaaa aattgttaat gcgcttgatc gcaagccaaa
     7441 ggatgaagaa aaagaaatta gttttcaagt tttagatgat gaagatattc cattttaaat
     7501 tggatggtga ttatgtgaga agtttagagg agagaaataa attagttgaa gaaaatataa
     7561 attttgtatg gtttattata agaagatatt tcaaagtatt tatttcaaaa tatccatatc
     7621 ttcaagatga tacattttca gaaggatgta ttggattatg taaagcagca gattattttg
     7681 atagtaaaaa aggtattgaa tttaccactt ttgcagctgt gtgtataaaa ggtcaaatat
     7741 ttaaatttat aagtaggtat gttaaaaaac attacagagc agatatagat agttttgaaa
     7801 aatgcatcta taaaaatact catggtgaag aaataagaat agaagataga ttggctggta
     7861 atgaaaatgt agatatagaa aatgttcatg ttattagatc ttgtataaaa agaagtaaaa
     7921 tagaagatat acagaaaata acatacttaa gggaaaaagg atacactcaa aaagaaatag
     7981 gcaaaatact taggactaac caaatgacaa tatgcaggaa gcttggaaaa ttaaaatctg
     8041 aaattaatat actaggaaaa taatataata atttaagttc attggaggca aaatgaagac
     8101 taaaataatt ggtggtaggg aaaatcagtg cccactttgt aatggtagtg tatttaagat
     8161 tgaaacatca attggaatag tatggcaatg taaggattgt ggatgtatgt atcaagatat
     8221 gaattgtaaa gaaaacaaaa ggaggcatta agtatggctt gtgttataaa gtgtgatttt
     8281 tgtggtgaaa caatagcaga aattctaaac ataaaggtat tcagaattga tgatgaaggt
     8341 aatataatga gtatcggaaa agatgtatgt gacaaatgtt atgacagatt atttagaagt
     8401 aacttgaata agaagatgac taattatgaa aagataaaaa atatgagcaa aatagaaatg
     8461 gctgaatttc ttgcaaatgg cggaagtgga tgtactagtt gtgcttatga ttttcaagaa
     8521 tgcttaggcg gatgtttaga aggtagaaaa aaatggcttg aaagtgaggc gatgaaataa
     8581 tgaaaaagac gttaataatc gtaggagtag ttatttcaat attaattatg tttgcttgct
     8641 tgttcttagt aactaaaaat aaagcaattg atttagaaga gcaggttcaa tcagcaactg
     8701 catctgtaga ggtgcaagaa aaaaggagaa ttgatttaat taagaacata gtagactgtg
     8761 taaaagacta tgctaaatat gaaaatagaa cattaaaaga agtaactgac atgagagtaa
     8821 gtgcaaatga tgaatctatc aaggaagcaa aattaaatat acaagcagta gcagagaagt
     8881 acccagattt aaaagctaat gaaaattata agcaactaat gagtgagttg agtataacag
     8941 aaaatatgat tgcacaacat agagaaaatt ataatatgca agttcgcaat tacaataaat
     9001 atataagaaa atttccagca aatttaattc taaatagtat gggatatgaa aaaatagaat
     9061 ctaaatatac tgaatataaa gcaaaagagg atgcacctca aaatttattt gattaagagg
     9121 tgcacatatg aaatttaaaa cttttgaaat aactaagaga gaatttttag tgagcataat
     9181 aattgcttgc atgatgattg tgcttggatt tttcattgat gataaagttc aatcaaaatt
     9241 tttagaagat aatgaaagat attacaaagc aactaaaata aataatgaca aagatctctt
     9301 tgattatgct atggaaacaa gtgcgggaga ttctttgata agtggaagat ttaaaagtgt
     9361 agattctgta tctatagaag aattaaaagg ggactattgg tttatagaaa gaacggaaga
     9421 aaaatacact atgcatacta gagtggttac cactaaagac agtaaaggcc attcacataa
     9481 atatactgaa acatattgga cttgggatta ccatgattca atagagaaaa aatgtaataa
     9541 atttaatttc atgggtaaag agtttgatgc gaaagtaatt actaatttat ctaaagagag
     9601 attggcatta aagaaagata tatttaaaaa taataatctt aaaataaaag gtaattatgc
     9661 ttacattaat aataagagaa ggtactatta tgaagttata ccgaaagaat ttaatggtac
     9721 tatgtatgct aatttaaaaa ataatactat agcgaatcca gctgataaaa acataaaagt
     9781 ttttaagggt gagtctatac agaatgtttt agaaaataat aattttaata cagggttttt
     9841 taaagtagtg ttttggatca tctggatatt aataactaca atcattatat atgtatttta
     9901 ttatttaaaa aatgattggc ttgaagataa ataaagatgt aagaggagaa gatcaggtat
     9961 gaaatgtaag gtgtaccatg ctattaaaaa tataaaagat gcaaatgagt taattgatag
    10021 atttgaaacg gaaaatatat tctttgaatt aaatgacata gataatgaaa atgtcttaaa
    10081 tggagaaatg aatttaactg atatagaaga tattgcatca ttgcaattta tattagatgg
    10141 agatttattt atatttagta gttgtgatga aatgatattt atgaataaat acactcaaaa
    10201 agagttggga cttcaataaa tgaatgaggg gagatactaa agatggattt aaagttaatt
    10261 agcagtatac ctaattggaa ggtagaagat cagttggcaa aaattgatga agaggtaact
    10321 gagtttaaaa atgctataga tacaggaata actaaagata taattgcaga aggattagat
    10381 gtttgtcaaa ctattttaac aatgtttcaa gttttagaaa tagaaaacta tattactgaa
    10441 ggagtagaga cacataataa gaaactaaat ggaagaggat gggatctcaa agaaatagga
    10501 gacgatatac ttaatttaat taaaaattca atgattaatg aattgattaa tatgaacaag
    10561 aaattaaaat gccttgatga aaaagaattt aatgcaaaaa tacaattttt agatgtagat
    10621 aaactatgga atttgtgcat gcctttagtt aacttaagta ctataaaagg agttaatgaa
    10681 agcattgatg aaatgaaaat gggagagttt ctaaaaaata ttaattaaag taaggagata
    10741 atataatatg ttgatagaag taactgaaaa taatagagaa ttaaaagtag gagatattgt
    10801 tgaaattaat ggtggtaata agttatatat gattctttct ttaaaggaaa caataggtgg
    10861 gtatgttatt ataaatatgc agaatggata tggttcattt ggtgcttata aaagtttaag
    10921 tcaactagaa ttagacttga aggtaagagg ttataaatta tatagttctg atgattacaa
    10981 gttgcaatta gtgcctagag aataagatat atttcaaaaa agaaaaaaag gagcattact
    11041 tcacgctcct gcttgtcaaa attctaaaac cgtcatcaca acatcattat aacataatta
    11101 ggagtgtgag gtaatgcaat ctagtaaaaa agacaatttg tttagtagtg cagaaggtaa
    11161 gttatatgat tataaaaaaa taaaagcgga aatagaaaaa atagacattg atatgcagat
    11221 gataaaaaac gactacagag gatgtagtgg agttgaaatt aaagaaaaga caggcaagac
    11281 atatgatatt aaaagtgtaa ttgaaataga gactgaagaa aaagaaaaga aaattgcctt
    11341 aaaagaaaaa gagaagagac ataaagagtt gataatagaa aaaatagata atgcaatgaa
    11401 aatattaagt gaagaagaaa aaaagatagt acagtataaa tacttttcaa gtagcaggac
    11461 atcttgggaa tatgttggca gaatgattgg tttttcagcg agtaagtgta agcaaatgag
    11521 gtttgatatt atagataaga taaaaggatt gctataaaaa tgtccgattt gtgacgattt
    11581 taaaccgatt tatgtctgtt ttgtgtctaa aaaatgtccg gtttgtgacc aatttatgtc
    11641 tgttttgtgt cttttaatca tgataagatt gtattgtgag aaaaatatat ttaataaaaa
    11701 tggcttggaa tttacgttct aagtcttttt tattgtctag agaggtgttg aagattaaac
    11761 gtgatataac agaagaaatt tcaagtgctg catatgtacc agacaatcta aagtattacg
    11821 acaatgtaat gaaagaaact cctggtatgt atggaagaga atgttcttta gattttataa
    11881 agaaaaagca agaaaaactc ttgaaactaa aaaagaaggt taagaaaaat tatgattcta
    11941 atattaataa gatagactca tatctaaaaa ttttagagaa aagtataatt gatgagtctg
    12001 atcacgttga gttggttgta tttaaattat atttgcaatt agaaaatgtt gtaaaagtaa
    12061 ctgaaactgt aaacgactta ggatttagaa taaagactaa cacttatgca agagaaagaa
    12121 aatatagtac aaatgatata acctctataa tcacagatcc ttttactgat gtagttgagg
    12181 acttaaaaat attagttcaa gagaggcaaa gaaaaaatta ttatggaaat aaggagtgtt
    12241 tttaatgaat ggaagcacat agtggacaaa aggttaatcc cataaaagat atagaggatg
    12301 tatttagact tttaaatttt ctagaagaat ggaatgaaag aaactattta ttagccctct
    12361 ttggaatgtg tactggactt agggtagggg atatattagc acttaaagtt gcagatgtaa
    12421 cagacttaaa actcgataaa aaaggaaata aaataagagt atctaaggat tggattagag
    12481 ttatcgaaga aaaaagagac tataatagag aggttttttt atctgatgta ataaaaagtg
    12541 ctatagaaaa ttatactcaa gataaaccag gagaagaatt tttatttaaa agtggcaaga
    12601 ggaagaaatt taatagacct atccaaacaa gacaagcagg aagaataata aaaggagcag
    12661 caaaaaaggt aggaataaaa gaaaatgttg caacacactc tttaagaaaa acatttgcaa
    12721 ggcatattta tgatgaagaa gagaataaga cttatgcatt agaacttata aggaagattc
    12781 taggacataa gacaatagaa atgactagaa gatatatagg aatagataag gatgaagaag
    12841 taaaagcaat aactaaattt actaataagt taaagaaaag aaaaagaaat taaaatatct
    12901 tgtattttat ctaaaattta atatgttacg tttttcaaca acaggacatt attagaatgc
    12961 aaagagcctt aacaatagag tttacaagga atagaataga tttattaaaa tgtctgaaaa
    13021 tatatattaa aggtcattta ttcaagggat ttatagggat gtagatattt aaatgtttat
    13081 ttagaagctg agtaaagagg tgattaattg gatgtgaatg aagttgaaat aatcgatagt
    13141 agctatgaaa aaggtaaaga gttgacagaa gatcagtgca atatggtaac tctattgatt
    13201 acaggtgcta cagtaacaga aactgccaga attattggag taaatagaaa aacaatctat
    13261 aattggatga ataaagagca tgttaggaaa gaaatggaca gacggaaaca ggagctaaca
    13321 aaccaaggaa atttaatgat attaaaggat ttagatagtt atatcaacaa tatcaaggaa
    13381 ttagctagtg acaagagcga taagagggtt atgttggcag ccaatcagta tcttataaat
    13441 cgtatatatg gaactcctac aagtacagtt atacaagcag aggataatag tgttggcatg
    13501 ggtatggatg caacagagat agaagcagct atagctaaga taagaataag aacaagcaag
    13561 aagtaattag atatgaaata aatcctgcaa gaatatgttt taattatgtt tgaaatcaat
    13621 taaagcaaaa catatattat atatatattt actttaataa ttaaatcata tattgtattt
    13681 aactttagtt atagctaatt taataaaatt tagtgagact taataattaa agtttcatac
    13741 agtgaagtga tgtggattat tataggatat gtgaacctat tggaaatgct aggttctttt
    13801 taactatgcc gaaaatacat atttaaggaa tagttgaaag ataattaata attctaatca
    13861 aaaaaatatt tcttgcaaga aaataattca aaatacttta atatatttat tttttttatt
    13921 aatagggggt gtccttctat tttgaaaaaa ttatttttta tggaaaatga gttctagaaa
    13981 ttttctagca aaatttaaaa accagagatg tatttcaaag agggtgattt tattagagaa
    14041 aaatttacat tgtttatttt attaattgca ttactcactg agcctaaaaa aatagaagaa
    14101 gtaagaggaa gtaaatacga gtggataatc tagaaagaaa tatacaatta atatttagtt
    14161 atctagcaaa gacttttata agaaatggag catctataaa agatgctgaa aaagaagcaa
    14221 atgagattat aaaagccaat tcaaacaatc tttggggagt taatggtctc gcttatcaac
    14281 ttggaaaaat aaacctagaa tttttctgta tgtattttat gcaagacaca tatttaccaa
    14341 aagaagataa tgctgctgct cctatagcta aagttcatca tgagctatgg gaagatatac
    14401 aagaatctat aattggagat ggttcacagc aattaggaag aatttatcca agagggacag
    14461 gtaaaagtgc atttggagat ttagcgacaa ctgtatggtc gcattgctat aaacataaga
    14521 catatacttt gatttgctct gacataggat ctacagcaga gaaatttgta aaagatataa
    14581 aaaatgcatt acttgaaaat gagtatatta aaaaagcatt tggtgtcctt ttaaatgata
    14641 atgatagaaa atacatttgt aatagtactc aactagagct aacaaataaa acttttattg
    14701 aagctatatc atcttcatca cccatgagag gaagaaaata caataataac cgtccagatc
    14761 ttatcatact tgatgattat cagtcagaag aaaatgttag aacagaagat gctagagaga
    14821 aaaaatttaa aagattttct gatgatgtaa aatatgctgc tcaaaaacca gtcataagaa
    14881 atggtaaaac tataaaaaga ggaacaactt ttatagcatt aggaacctta caacacaaag
    14941 aatgttttta tagtagacta aaaaatttac caacttggaa gtttaaatgc gaaaaaggag
    15001 ttttagttga taatgtagat gaattattta attctggttt atggcttaag ttcaaagaga
    15061 tcttatttga ttttaaaaat acaaatcatc ttgaagatgc taaagaattt tactgggaaa
    15121 atgaaaaaga aatgaaattc cctatattat ggccaagttt ttgggattgt ttagatatgg
    15181 ctctaagtta ttatgaaaat ccaacatctt ttaagcagga agtgcaaaat gatgtcaatt
    15241 ctataggaga aaaatggttt aaaactgtta gaacagaaac tagagaagaa attgaatcgc
    15301 atacttttaa aaagacaatg ctgttatgtg atccagcatc tgcgggtggg tcgaaacatg
    15361 actatagcgc ttttcttgtt ggaagtgaat cagaaaatgg tttactatat ggaagattgg
    15421 cagaactagc taaaataaat gctagaactg attttgataa atatatagac catatgattt
    15481 atttattaaa agtgtattca gatataacac atatttatat agaaaaaaat acatttaatg
    15541 gcgcagatgc caaccaatta gagtttaaaa taaaaaatga taatgttctt agttatagag
    15601 atatagaaat cattaatgag caacaaaaga aaaataaaga tgataaaatc tctactttaa
    15661 tacctgtttt aagcaaaggc caaatgattt ttgcagaaga agataaggaa tttatacagc
    15721 aaatacttga ttttacagga caaaaatact ctctacatga tgatgctccg gatatatcat
    15781 cggaatttat taatcgaatt aacaacataa aagtttctga gacagtaaat atctttgata
    15841 gaaaattatt aggtttttag ttgggaggta atttattggc taatagatca aagtttgaag
    15901 aagttcaaaa aactttaact aaaaaaggaa agaagttcaa tgttggaata ggaaaagatg
    15961 aaaaaggata ttttgcatat actcatagag caaggagtaa aagttatatt tcaaagcaag
    16021 atattccaat aaaagtttta aaatttatag agtctacagg ataggatgtg ttgttaatgg
    16081 aattagattt agacttaata aaagacatat atgacgattg gaactctaat aaatccgaat
    16141 atgatactat gtataagtat tataaaggtg aaacggatgc tattagcaac tataaaatgg
    16201 ttaccaagag atctaataat aagataaaca ctaatttctt aaaaaagttt attaatgaag
    16261 aagtcgctta ttctcttgca aataagatta cctatacaag tagatctggt gatgagaaga
    16321 ttataaatga tttagaatac tatacttgcc attggagtaa aaaacatgac tcagatttat
    16381 tgaggtatgc actattattt gggctttgct atgaacttta ctatgtaaaa gatgatgaaa
    16441 tacaagtaaa gataatcaag ccaacagatg gctgtcatta tgaaaatgaa aatggagaaa
    16501 tagtatgctt tattagagag tttaccaaag gtttcgaaga tgacacgtat gtagatgtat
    16561 atgacaaaga atatatatat cattttgatt caaatttcaa agaagttgaa agtcctacag
    16621 taaacaatat atttgatgga aatgttccaa tatcaatttg caaaagaagt gaagaactag
    16681 gaaaaaatac aatatttaat gatataaaag ggttacaaga tgcttatgaa actaatttaa
    16741 gtgatataag taatgaaatt agtgatttta gaaatgctta tttaaccttt tctggatgta
    16801 atattaaaga agatgatttg ccaaggatga aagaacttgg aatacttcaa gttaatggag
    16861 atggaaaaat agagtggctt attaaagata tgaatgatac atttgttcaa aatacactat
    16921 cttcaataaa agaaaatatg tatgaaatta catctcatat aaatcacaat gaaaaaatgc
    16981 aaagtaatac atcaagtcta gcagttatag caagattaat cagtacagaa tggatttgta
    17041 gccaaaataa tgatagtatt gcagatactt tatttaatag gtacaaatta ttgtgcattt
    17101 ggcttaataa aaaatatggt tttgactatg attataaaga tattaaggct aaatttaccc
    17161 ctaagatacc acgtgatgat ttagttgtag caaatatatt aagccaactt ggagataaat
    17221 tatctactga aactggctta tctcaactaa gtttcattga taatcctcat gctgaaatag
    17281 aaaaagctaa aaaagaacag gaaatagttt cagagggaga gattttatta gatgaatcaa
    17341 aagattataa ctaaattgac tgaagatata tataaatctg ctgaaaatag gacaaaacca
    17401 ttttataagt atcaaagaga aaatagggat aaacttttat tagaaatagc aaagatattg
    17461 ctaacacacg atattattaa tgaatactta aatattaata ataaagataa gaaaaatcta
    17521 aggtcaaaac ttaataaaat tataaatgag tattctaata gttatgatga agaagtcaag
    17581 gatatagaaa ctattttatc tgattcatct aagaataaat attcaaattt gataatttta
    17641 ttaggagtaa tactgaagat aaaagatgtt agtaatataa acaataaagt tataaataag
    17701 atagtgaata ataaggttga tggaaaacat tggagcgata gactctggaa gaataaaaaa
    17761 ggtatagaag agtccttaaa aaaagaaata aagagttttc taaatggaaa aacttctgta
    17821 aataagatag aaaaagcaat aagagataaa tattcaacag gtgcaggaca aaccagcaga
    17881 ctaatagaaa atgaagttgc cagatgtcaa agtgaagtaa atgagtattt tttcaaggtt
    17941 caagggatta taaaagtaat gtattgtgca gatttagatt ctcgcacatg tagtgattgt
    18001 tctatgcata atggagtgat attttatgtc aatgaagata ggccctcttt gccaagacac
    18061 tgtcactgta gatgtcaata tatacctgtt taacatgtat taattaattt aataggtgtt
    18121 tttattatgt ctaaaaaata tgaggtgaaa tatgaaagtt gaaatattag gaacagaata
    18181 tctagtgata gagaaacttg aaagcgaaga tgtgctatta aaggaaagag ctggatattg
    18241 tgatcactct gttaaggaaa ttgttataga gaaaataaat tctgaagaag gttcattgaa
    18301 agatttaagt gtatataaaa atgaagttgc taggcatgaa ataattcatg catttcttag
    18361 tgaaagtgga ttgaagagtt gtagttcttg ggctacaaac gaagaaatga ttgacttctt
    18421 tgcaattcaa tttcctaaaa tagtaaaggt tatggataaa atagaatgtt tgaattaaat
    18481 tttcaaaaag gagaacttta tacatgttaa aaaaagaagt gttggaatta ctaaaagaaa
    18541 tagaagatga tgcagaaata gattcattgc tgcaatcaac agatttattc aaatctgcat
    18601 ctgataaaaa gcttacaata gaagagttta gagaattaat taataatgat gcaaacttta
    18661 aagctattat agataatgaa aaaaataaat atcacagcga agcacttgaa aactttaaga
    18721 aaaaagatat gcagacactt ataagtgctg aagttctaaa aagaactgga gcaaatgaaa
    18781 cagaagagca aaaagcaata agggaattaa gagaaagcct agacaaacta aaaaaagaaa
    18841 agcagtacgc agaaaaagtt tctaaatata aagatatttt agttgaaaag aaaataccta
    18901 caaacttaat tgaatatcta ttatctgatg atgatgataa aacaaatgca aatatagaga
    18961 tatttgaaaa ttcaatgaag caatatgtgc agtcaaaggt agatgaaaga attaaagatg
    19021 gttcatacgt tccacctggc aaagatagtg caggttcgct atcagaaatt agaaaacaaa
    19081 taaagcaagg gctaaatagt ctttagtata aaaaaagaaa ggatgatttt ataaatggct
    19141 aatgtattac aatatgtaac actttttcaa caagaactag atatgcaggt aacagttggc
    19201 tcaactactg gatggatgga gaaaaatgct ggtcaagtta tatataatgg aggaagagag
    19261 gtaaaagtac caaaagtaac aatggatggc ttaggtgatt atgacagatc taaaggatat
    19321 acgcaaggcg cagttacact tgagtacgaa acaatgaaaa tggagatgga tagaggtagg
    19381 acttttatgt tagattcaat ggatgtaaat gaaagtaact tcgttataaa tgcatcttct
    19441 gtaatgggat tatttcaaaa gcaacatgtt ataccagaaa tagatgcata tagatactca
    19501 aagatatttt ctgaaacagc taaagtagat gagcgtgtaa aatattcata cgaagctaca
    19561 gtaactacaa tattaagtga attgaaaaat caacttgcat caatacaaga tttatctgga
    19621 gatatacctc ttattattag tatgtctagc ataattgcaa gcatattaga acaatcatct
    19681 caacttgatg atattgaatt tggcgatgga gatataaaaa gaattgtaaa gtctatagac
    19741 aatgtaccta taataaaagt tccatcaaaa agaatgaaaa cagaatacgt atttagagat
    19801 ggtaaaacat ctggacaaac agagggtggg tttactgaag gagcaaaggc tttagatata
    19861 aactggttaa taactccagc atctgctcca attgcagtat ctaagactga taagccaaga
    19921 atatttactc cagatcaaaa ccaaacagca gatgcttatc aaatagacta tagaaaatat
    19981 cacgatttgt ggatattgga tgaatattta aaactttgta gagcatctgt gagacaagct
    20041 aaagctgcac aagaataagg agttgtttta aatgtttgca ttgataaaaa ataacataat
    20101 taaagttgtt gaagatgaaa attcgagaga taaattgatt tcaaaaggat ttaaaatata
    20161 ttcagaagaa gtgactaaag agaatattga gaaaaaagaa gaaaaagtta aatcaacctc
    20221 taatgattta tttaatctat ctaaaccaga gcttatggaa attgctaaag gcaagaatat
    20281 aaagggatat tcaactaaaa ctaaagagga attaattcaa ctactagatg agtacaatgc
    20341 ttgataatgt taaattagtt ttaggaatag aaaatgatag atatgacaaa ttaataatcc
    20401 tttatataaa taaaataagt gagatggttt tagaatattg ttctaggcag gaactaagta
    20461 cagcactaga agggtttgta gaagaaaaag taatatcaat attatcttca agatttgaag
    20521 aacttaagaa aaatagtgga cttgataatc tagaaaaaag cttaaaagct ataactagag
    20581 gtgacactaa gatagaatat aatgctttaa cattacaaac tgctgaaaat agcatcagtt
    20641 catcatcttt tttaactgat actgataaaa aatttctaag cccattttgt attgttagta
    20701 tgtactaagg ggtgattaaa tgactgaagc agatatatta gaaatgacat accttgataa
    20761 aatgactata attagaaaat taaaaggctg gaatgaagaa acatgttcta atgattttag
    20821 taatgttgtt atagctgaaa atatcccatg tgcaatttct agaaaagaag aagccattgt
    20881 aaatggtgat attggatcta tagttgtaaa tagaaagtta ttttgtagac cagaagtaga
    20941 tataaaaata ggtgatacta taaatacaac ctataatcat ggagaagaag ctgtatttaa
    21001 ggtttctaag gcaaatatat atccaagcca tatggaaata cctattacag aattggagag
    21061 gatataatgg gaatagataa tttaggagtc tttgaagcat tagccttaga tttaggtcta
    21121 aatgcatcaa actttaatag aaaaatcaag aagtttacaa aagaaactgg gcaagaacta
    21181 gcagaaaata taagagatga aactcctgta aaaactgaaa agttaaaaaa gggatgggaa
    21241 gaaaagcaac cagaatatga tacttatatt gttaaaaatg aagttgatta tgctacttta
    21301 gtaaataatg gccattcaac taaaaatggt ggcattgtcc ctggttcttt tatggttgaa
    21361 aagggaattg aaagaacaaa agaaaatatg acaagtgcaa aagcaaattt atttgattgg
    21421 taggtgatta gttgcttaca aacagagaca ttataatagc agttaataaa agaatcgaaa
    21481 atgagtttag atatatagat atttttatta atgaggataa tatacaaggc tttgaggagg
    21541 cttgtttttt tgtgcaactt ttacctgttt ctaataatat tgcaaataaa gttttagata
    21601 ctaaaaagat attcatagat attgaatatt accaaaaggc tagaaaaaat aaattaaatc
    21661 tatacgacat acaaagtaga ttagaaaaaa tatttaacag aaacattaga gttaaagata
    21721 gaagtttaat cattgagaat atatctccat caatcgcaaa agatcctgta ggatataaac
    21781 taacttttct aatgactgta tcttattttg aagaagtcta ttttgaaaat gaaacatttg
    21841 aaattatgga aagtgtagat ttagagatag gaagtgatta aatgggttta ccagaaatta
    21901 atatttcttt tatccaagca ggaaaaactt ttgttaaacg ctctggtggt attgtagcgc
    21961 ttattttaaa agatacaaaa aatatagggt tagttgagat aaaagaaata gaggatattc
    22021 cagaaaatta ttctgctgct aatttagatt atataaaaat ggctatgaag ggaaatgtat
    22081 atcctccaaa taaagtatta gtttatacat tagatacaga tgaatcaata gatgatgcac
    22141 tagattttct tgaaacttgt gagtttaatt atctttgtat gccagatgag acagaacaag
    22201 atttgcctaa aatcaaaacg tggattaaga aaatgagaga agataataaa ataaaggtta
    22261 aagctattac tgcaagtaat tcagcggatt atgaaggtat aataaacttt acagcaactg
    22321 atattgaagt tgaaggtaaa aaatacactt caaatgaatt tctgccaaga atagcaggtt
    22381 ttattgcagg aactccatca actcaatctg taacatatgc agaaatacca gaagttacta
    22441 atatacctaa gctaacaaga gctgaagcaa atacaaggat aaataatgga gagctaattt
    22501 taataaaaga aagtggagct atagtaatcg ctagaggagt tacatccttt acaacaatta
    22561 ctgattcaaa aggtgatata ttcaaaaaga ttaagttggt tgatacacta gatcaaattc
    22621 ataacgatat taaaaaaata attgtaaaaa attatatagg aaaaacccaa aacacttatg
    22681 ataataagtg tttacttatt gttgcaatac aattatatct tcaagaatta gaaaaagatg
    22741 gtttaattga tgaaggttca actgtagaaa ttaatttaga tgctcaaaga gcatggctaa
    22801 aaaaacaaaa tatagatata tctaatatgg aagaacaaca gataaaagaa tacaatactg
    22861 acactcttgt atttttaaaa gcaagaatta aattaataga tgcaatggaa gatgtatatt
    22921 tagacatctc tatgtagttt ggaggttaaa atttgaaagc taaaatagat gcaaaaagaa
    22981 taatatctgg tacttttgga gagtgttggc tagataataa gcaacttaca tcaacaaaag
    23041 ggatggaggc caaggttgat atagataaag aagaaataaa attatgtggt caaataatga
    23101 ccacatataa aatgattggt gcaactggaa aaggatctat aactctttat aagataagta
    23161 ccttatttgg cgagtatgtc gaaaaaatgt taaatgaagg taaagaattt aaagtaacta
    23221 taataagtaa attgcaagac ccagattcat atggttctga gagaattgca ctttatggat
    23281 gtatgtttga cgacttaact ttggctgatt gggaagctgc gaaagttgga gaaatagaag
    23341 ttccattcac atttgaaggc tttgaatact tagataaaat aaaagaaaaa taggaggatt
    23401 ttattatatg aacacagtag aaaaattatt aaatatggat gcaggtaaat taaaaatgcc
    23461 atctagcgca tttgaattat attgtaaaaa actagatgat actttagaaa tagagtgcaa
    23521 ggctattgat ccagaacgtt ttgacgaaat aagaatgaac tctatggacg ttaatagtgg
    23581 agatgtagaa aacattaaca tatatgaact taaagtaaat acaatattag aatcatgttc
    23641 tatatttagc aatatggaat tagttaaaaa atttagtgca attacaccta aagaattaat
    23701 taaaaagcta ttacttagtg gtgaaataga taaattatac gaggaggtta ataaagtaaa
    23761 tggaatagat gcagagggag ataaaaaaag acaaaaagaa agagctaaag atataaaaaa
    23821 ctaattaaaa cggatggaga agttaacttg atgtacttat tatttaagta taaaggtata
    23881 atgccatccg tttcttatca tatactaaga ggcgaaaaag aaattgttag tgtttttatg
    23941 gatcaagaat tagatgaaag aaaagaagaa ataaaatctg gtgttgggag gttgttttag
    24001 tatatgagtt ctaatacaaa ggtcctacaa gcaataataa aaatgagaga tgaagcaagt
    24061 tcaacattaa aaagagtagc agcaaatact agagctctac atgattcatc ttcccaagca
    24121 tcaagagcat taaataatgt tcaaagtaat ttaaaaagtg ttggaactag tgctttaaaa
    24181 gcaggaggtc ttatcactgc tggagttggt ggtgcatttg cagctgcaac taaagttgga
    24241 acagaatttg aaagcgctat gtcgcaagtt aaggctacat ttggaaattc cttaaaacca
    24301 ggagattttg aagctctgtc taaggctgct agagatgctg gaaaagctac aactaaaact
    24361 gctgctgatt ctgctaatgc acttcagtat atggggcttg ctggatggga tgttcaaaag
    24421 tcaatgcatg ccttaatgcc agtgcttaat ttatcagaag cagcctctat ggatcttggt
    24481 ctagcatcag atttagtaac tgatagcatg agttccatgt gcttagaagt tgataaggtc
    24541 agtaaaagtg gaaaagtact agctggtgca ggtttaagtg agtatttaga taaagttgca
    24601 aatgcatcta ctaaatcaaa tacaagtata caacagttga tggaagcgtt taatgtcgca
    24661 ggtggtacat ttagcaattt aaaagttcca ctttcagaag caactgctat catgggaata
    24721 cttgcaaata gaggagttaa gggtacagat gcagggcatg ctttaaactc tatcatggta
    24781 aatcttacaa caggtgcagg tcaagcaggt aaggcaatga aaaagttagg tgtaagtgca
    24841 tttgataatc aaggtaaatt taaaggtatg tcaaatgtgc ttaaagaggt tgcaaataaa
    24901 actaaaggta tgactgatga acagaaaaat tattacttat ctgctattgg tggaaagact
    24961 caattaaaaa cgttacaaaa gttacttgat ggtgtgggaa atgaatatga tagcttaaag
    25021 aaaaaaatag ataattcaaa aggtgcttta aaaacaatgt ctgatgtaat gaaggataat
    25081 actaagggtg gacttgcaac cttaaaatca gcccttgaag aaactggaat tactatttac
    25141 tatcaactaa agccagcaat agcatctgta gttgataatt taactaaaat gactgtttgg
    25201 tttaataatc taagtgaacc aataaaaaga gtcataacta atgtaatgct ttcagttgga
    25261 gcatttggag tacttttatt aactataggg gctgtgtctt taattgtttc aaaagctata
    25321 ggtatatttg gcttttttgt tccaataata ttaagagcaa aagttgcttt cagagctttt
    25381 gtaattggta tgaaaccatt aagtgcagta tttttagctg taatgggtcc tgtgggatgg
    25441 actataacag ctgtaattgc attatcagta gcatttacca ttgcttatgc taaatctgaa
    25501 gtatttagaa ataaggttaa ttcattagta aaatcattta cagaatttgc aaagcctata
    25561 gtaaattttt gtatgcctgc acttaaacag ctaggagaga aatttaaaga gttgttatca
    25621 gcatttggtc cactatttgc atctatttta aacctaggaa gtgtaataat gaaggtacta
    25681 ggacctgttt taatcggcgt aataggtttt catatagcaa aattagtttt gattttatct
    25741 actctggcaa ctgcttttaa agctgtagtt gatactataa ctaatgtatt aaatggtcta
    25801 gcatctattt taaatggagt atttgattta attataggta ttgtcacagg cgatagtaaa
    25861 aagatgatgg atggagttaa acaaatattt gatggtggaa ctaaaattat aaaatcaatt
    25921 tggaaaggtt tcatagattt tgtaactgct cctatacaag ctatagctga tatagctgac
    25981 agcttattta aagataaagc taaatggctt acagataagt ggagagattt aagaacattc
    26041 ttagaaaatc caattgaagc aactgccaaa atatctacta aaacaaatgt aaatatccca
    26101 ggaagtaaag cagcagatgc agctggtaaa tctacaccaa atacaggtgg attaaaacaa
    26161 acagtagcaa atctaaaagg ttcatttagt gattttaaaa cttttattaa gcctatgaca
    26221 gactctataa gtcaaggagt tgaatctgga aaaactaaat ttacagaact gcaaaataaa
    26281 atattaaatg atgttaaacc tgcactaaag gtcctagagg attctggagt aaattttaag
    26341 gataagttta aggagtcaat aacttcaata aaagataaat ttaatgaatt aaagaacata
    26401 ttagctggaa atattaaaac aaatatagat aatttagtaa aagcatttga acctttaaaa
    26461 cctcatttag ataatttgaa agattgcttt gataaggtta aaaaagcgat tactgatttt
    26521 tttactccag ccaaacaggt tggagataca gtaaacaaag taagccaacc aatggatagt
    26581 gttaaaaagt ctactgatgg aattaaactt tctttccaaa gtttaactca agcacttgct
    26641 cctgttaaag cttggtttca aggatttatg cctttcctat cacaaatagg acaacaactt
    26701 ttaccatcta taggaattgc aatagctggt gttgttggcg gaataatttt agcatttaca
    26761 tgggttttaa atacattgac cgcaataata acaacagtat ctggaatcat aaatggaatt
    26821 atgtctttta tatctggaat tattaatgtt attatggggc ttataacagg aaatgctgat
    26881 caaactatgg atggacttaa acaaatattt gatagtggag taggtgtaat aaaatcaatt
    26941 tggaatggcc ttgttgatct agttagtggc gtactagaac ctgtaataga tcttgctgat
    27001 tctcttttca aagataaagt taaaggggta aaagaagctt ggaataaact taaagatttc
    27061 tttaaacatc caattaaggg aacaatagaa atatttaaaa aaggtcctag tttacaaatt
    27121 ggaaaaggtg cagatgctgc tggcaaaagt ccaagaaaag catttggtat aaatagagtt
    27181 cctagaaatg attatttaat aagagcacac gaaggcgaga aacttttaac caaacaagaa
    27241 gctaatcaat acaaaaggaa tagttcaaat ggaagtttat ttcttgataa attggctgat
    27301 acaataattg taagagaaga ggctgacata gataaaattg taagaaaaat aaacaaaaaa
    27361 atatctcttg caaaaattgg aggtgttttg taaattatcg tgctataatt gtagtatata
    27421 atttacaggg ggttatgtta tgaataaaaa aatactagta ttaacaatat caattatact
    27481 atctttaagt ttagttggat gcagcacaaa taattcaaaa aatagagata gtaaatctaa
    27541 gcaaaacaca gaggaagtta aaaatcataa aataggcgaa gttgttgatt tagatggagc
    27601 aaaagtatct gttaaaaata ttaggaaatt agaaacaaat agtaatgatt atgctgttga
    27661 aattgaaata actaatacag gtgataatga tttacaaagt gattatctag aaaactatat
    27721 attaaaatca agtgacaata ctactggaga agcaataaat aaccaagaat taaatggaga
    27781 gttaatgttt gatacaatac gcccaggaga tactttgaaa ggtgaaatag cattcgaact
    27841 tacaaaagat gcaacaccaa aaacacttga aattgcttat gaaaatgtag gcaaatatac
    27901 agtatttgat ttatagtaat tatcttaaaa gacgcttatt tacttaagtg tcttttattt
    27961 taggaggagt ttaatggaaa tatggttaag acaagcaaat aatacattta ggtttcctgt
    28021 tattccaccc tcttttgaga taaatggaag tgcgacaata aatacatcta atattttaag
    28081 tgttggagat atcgcagtat ttggaggatt agggcttaaa actatagaac tatcctcttt
    28141 tttccctaat caagaatata gtttctgtaa ttataatgga tttccaaaac catatgattg
    28201 tgtaaattta atagaaagtt ggatgaaaga aggatatata ttaagattta tcattacgga
    28261 aactaatata aactttgaat gtataattgc agattttaat tatagagaac aagattattc
    28321 aagagatatt tattttacct taagtctaaa agaatacaga aggatacaaa tatccaaagt
    28381 aaacattaat aatgatgaaa aattatcttc tgaaaagaat gttcctctaa caaaaggatt
    28441 tgatactaag caaaaaacgc ataaggttgt tgaaggagat actcttttta agatagctaa
    28501 aaaatattat agcaatggag atttatggga gaagatttat aaagcaaatg aagatgagat
    28561 taaagatcca tctgtaataa aaaatggttg ggtattaata attccttaaa aatggtggtg
    28621 atttataatt aataaaatca aaatacaagt tcatataaaa aacggaaata tatataatgt
    28681 aactgattta gtggataaat gcacttggtc tggtgattat agatcaccat caagaacttt
    28741 ggaattttct ataattcaat ctgcaagtga tattaatttt aggcagatag atattccagt
    28801 agcaagtaca gtttgtttct atgtagatga taaagaactt tttagaggaa tgataataga
    28861 tagatctaaa gactctagca ataataatat agattttaca gctaaagata tgggtttttt
    28921 acttttacaa agcgaagtgt catacaattt taaagataaa ttagtcgaag atattgcaaa
    28981 acaggttttt gtagataata aattcccact aggtaattta cctaaaacta acgttaaata
    29041 tacaaaaatg tttattggtg taactggcta cgatactata atgagcgttt atacagaagc
    29101 aagcaagaca actaaaaaaa agtatatgat agagtctaat cttgataaat ttaatgttat
    29161 tgagaagggt gtagttactt taaatataac atttgaagaa ggctttaatc ttatcaatac
    29221 aaatttttct gaaagcattg agaatgtaaa aaataaagta ctagttgttg atcagtatgg
    29281 taataaaatt agtgagaaaa taaatgactc tatatttaaa gacgttgggg taattatgca
    29341 aaaggtaata caacaacaag aaaacagtac tatagacata gatggagagt ttaaaggaat
    29401 cgaaaaaact tgctctttaa aaggatatgg tgatataact tgtgtaactg gtagaggtgt
    29461 aaaagtaaaa gactcatata caaaattaat aggtcttttt tatatagata cagataagca
    29521 tacatggcaa aatggagatt atcaaattga actagagctt aattttgaga atataatgga
    29581 tgaaaaatct gctggccaag atgagcaaaa agaagatggt tcagagtata ctggaggaac
    29641 agaataccct gctgaattta cggcttattg tcctagaaaa gaagagggtg gaaatacaga
    29701 ttgtagaggt aaaaaactag atccttctaa aaagacatgt gccgctccta tggttggagc
    29761 atatgaaaaa tcttactata caaaagattt cttaaacaaa catcctctta tgagatatgg
    29821 agatgaaata caattagtta caggtgtttc aagccgagat ggaacgtata aagtaaatga
    29881 taatgggcct gcaataatta tagaaaagga tggaacatat catatagata ttttatttgg
    29941 aaatgttgaa gaagctaata actttggaag aagaaaagga aagattataa ttggtggtta
    30001 ttctggaaac atgacagaaa aagcaaaaac agtaatttca gaagcaagaa aacatcttgg
    30061 aaagccatac aaatggggtg ggaatggacc aagtagcttt gattgttctg gactaatggt
    30121 atattgcttt aagaaggtta atgttaattt accaagaacc tcaagtcagc aatctaaggt
    30181 aggtaagaaa gtagagaaaa ataatttaca ggcaggagat ttggtatttt tccataatcc
    30241 agtcagccat gttggactat atgtaggtaa tggagaattt ttacatgcgc cacaaacagg
    30301 agatgtagtt aaaataagta aattaagtaa tagaaaagat tttaatacag ctagaagagt
    30361 tttgtaaaag aggtgatatc gtggcagatc caatcaatga atttatagga ataattaggc
    30421 aagaaggtaa atttcataat gaaccttctt tttttattgg aaaaattaaa agtaaattac
    30481 cagatttaaa aatagagata aataacatca tattagaaaa agaagatatt ttgatagata
    30541 gttggatgct tgatagacag atagagttat ttgatacaga aacaagtcaa gaacataaac
    30601 atgaaataaa aaatcccttt atagatactt ttgaatctgg aaacacagta ataatgttta
    30661 aaataggtga aaaatttgct gttgtaagta aattggtgag tctagatgaa taatactata
    30721 ttccctttca tgggtgttcc agaagattat atttctccag acaatgaaga attacctatt
    30781 tttaaagaat ttgcttggga ttttaataaa gatgaaaaga taattgaaaa tggagacttt
    30841 aaaatagttg aaagaaatga agcaattaaa gtatggattt acaaaacaat aaaaacagat
    30901 aagtatgttc atttaatata tgattggagt tatggaacag atataaaaaa tctaataggg
    30961 caaaagtata ctaaaggtct tactgaaagt gaggcaaaaa ggtatataca agaagctcta
    31021 ttaactaatc catatatatt agaagtcaat ataacaaatg cagaatttga agatgataat
    31081 ttatctataa gtttaaatgt aaaaacaatt tatggagagg aggagattac ttttgtataa
    31141 tggccaatca ttcagtacct taaataatag aactttaaat aacataaatc tccctcttta
    31201 taaaggccaa ggatcttctc tttataatat agtttctcca ataaattcag aacttgcaca
    31261 attatatata gaactttctt atatacataa gagagttttt attcaagata attttgacga
    31321 ttttcttgat agaagagtca atgaatttgg cgtatataga aaattaggta cagaggcaat
    31381 aggtgaggta acatttgagg gcaaaattgg aactcaaata ccaaatggaa caataatatc
    31441 tcatagtgat ttactatttg taattattaa agatattaca atagatgaaa atagtaaatt
    31501 aaatgtaagc cctatacagg ctttagagat tggcattaag tataatttat cagctaacac
    31561 agaatttaaa cttatagaag aaataactgg agtaactaaa atttataatg aacttgacct
    31621 aaagggtgga acagaaatag aaactgatga agaattaaaa gaaaggttct ataaaataca
    31681 aaaaaatcaa gcgacaagtg gaaataaggc gcattaccaa tcttgggctt tagaggttga
    31741 aggtgtttat aatgcaaaag taattcctcg ttgggatggt ccaggtacaa ttaaaattct
    31801 aatttatggt caaaacaatc aatcagttga caatgaagta ttgcaaagat gtactgaaca
    31861 tatagaagaa gaaaaaccta ttggacctac tgttacaatt gttacaccaa gtacttttga
    31921 tattaatatt agtgcaactt taacacttga aaatggatat gatattgaat ctataaaagt
    31981 tgtgttttta gatattataa attcttattt gatagaaaat tcaagagaga ttgtttatat
    32041 aaaagtaatg agtttgttag caagcataga aggtattcat gatataaaaa atctccttgt
    32101 aaatggtgat gttaaaaaca taatagttga tgaagaaaaa gttcctgctg tttcaagtgc
    32161 tatatttgat atcgaggtga gttaaattga agttaattga ttacctgcca agttttgcaa
    32221 acaatgaaat tgacatacaa atccaagaag cacttgaaaa tgaactttta acgctaattg
    32281 atgaaaaaga tgatttatta gaacagtttt ttatagatac agcaacttgg ggacttgatg
    32341 attgggagaa ccttttaagc ataaaagtaa attataagtt agacttcgat actagaagaa
    32401 gcaatataaa agcaaagatg agaggtaagg gtacaacaac aatagaggtt ataaaagcta
    32461 tctcggaagc ttacacaaag accaatgttg atgtagaagt atttagtaat ctatttagtt
    32521 ttacacttag ttttataaca aataattgta gttataacac tattttagaa ttagataaga
    32581 aaatagaaga aataaaacct gcacaccttg aacacaaatt cgagaggata ttatttaata
    32641 aaaacgagct ttatacaggt gcagcaatta gtacaggaga aacagttaca atatatcctt
    32701 atgtacctag aaatttagaa agttttggag aaatagctat ttgtagtgga aatgatagag
    32761 cattagaaaa agtaacattg tatcctaaaa aatagaaatg aggtgataaa attgacagaa
    32821 caacaatatt ttactctagt aactgacatt ggtaaggcag caatagcaaa tgcaagtatt
    32881 acaggtgaaa aagtagattt tgcaaagata aaagttggag atggaggagg gagttcttat
    32941 actccaaatg agagtcagac agcactaaaa aatgtggttt gggaaagtac acttgaacat
    33001 gcacaaggag ataaagataa tcctaactgg gtagtaatac aaaaattcat acctggtgat
    33061 gttggaggat ttgaaataag agaagttggt ctatttgatt ctaaagacca attattagcg
    33121 atttctagtt acccaacaac atataaacct actgcggatt cagggactgt aaaagaacta
    33181 ttaataaaag taatattagt tgtatctaat gtagctaata ttaatttgaa agtagaccca
    33241 accgtaattt tagcaacttt aaaagatata caagaattag acactaaaat agatacaact
    33301 aaaacagaat taacaagcaa catagaaact gctaaaacag agttgaacac taaaatagga
    33361 gatacaacac aacttactac aacagataaa acaaatatcg ttagtgcatt aaatgaggta
    33421 aaaactagtg tagatagtat agaaacaaca gcagataaaa caagtataaa agatacagat
    33481 aatttatttg agagcgacaa cgtagaggga gcattaaaag aagtgatgca agaagtaaaa
    33541 ggtaatagaa gcagtattat atcaacaata aatagtaatt taataccaat gtagaaagtg
    33601 aggtgataaa gtgaagatat ggaaatacaa tagaactgtg aagaaaagaa gaggtgtcta
    33661 tacagaatta gatatacttg cacaaaggta tgaatttgaa gcagatggga aactttatac
    33721 agcttatttt catttatata ctaataagaa tatagatata ttcagagata aagatttata
    33781 taaagaatct tatatgatga gctatagagt tggaaatgta acaactgata ttgatgatgt
    33841 agtacagcaa acaactacag taagatttat acctgaccct gttattgata ttgtgattga
    33901 taacattagc agaggatgtg aaataaacta cagaattata gataatgaac caagtgtaaa
    33961 attcattgtt acagaaaagt taaatggcac aataatagaa acaaagacaa attctagtga
    34021 taataattat aaacttaaat taactgatga acaaatagta ccattagcat tcaattccca
    34081 aaattcaata gtaatagaaa taagtacaga agatggtgga ttagtaacaa gtaaaacagt
    34141 aacatttaca agaacaaata acaagccaac aattttagtt aattcatata gttctaattc
    34201 tgcaaaattt acagcatctg atttagataa taatttaagt aaaattgagt ggtatttaga
    34261 tgatgtatta aaagaaacaa taacaacaga tttaacagca gaaaaaacca tcaactatga
    34321 atttacagac aatgcaatac acacattaaa aatagttgct acagatgcag aaaatgcaac
    34381 agttgagaag gttttaagta taagtaaaga gataatgcca cttcaatctg atgctagttt
    34441 aagtgatata tcaactaagc tgatagagat tggagaaggg tttagaaatg gtaaaacaag
    34501 tattataaac actttagcac ttaaaaatat agaagcaagt ttaaataata cacttgtgga
    34561 gttatcagag aaaataaaaa caagttttga tagttcagac gctagtgtgc aggatttgca
    34621 aaatagaata acagaattga ataatcagct tagtcgaaga aaaaaatggg ctacaggtag
    34681 atacacattt actagagaag aagatactat caatttcaga atggttgatg gagatagtct
    34741 ttccgcagta aaaaaactaa ttatacccat taatttagat tttgttccaa gtgttattat
    34801 aatagacaaa atgtttttag gtagttacgt acctgcacaa gatgtgcaat ttaattgtgt
    34861 ttcaaactta gccgatttta cacttggacg tcgtttctat aataaacaat ggatttgcac
    34921 ttcttctata aaactagatt cagttacaca aaatagtttt actctttctt tacaaaaagt
    34981 tgttccattt gataaagtta gtagtacgat tttttctatt attaatactg gttttgcttg
    35041 gagagcatat gaataaaaat ataaattaga ggtgattaaa tgaatagaaa taatagaata
    35101 atttacgacc aaacaggcaa gatatggctt caaactggtg aagcaatagg agatatacaa
    35161 gagttgtcag aaataactga attaaatttc atagatgttg aatttggtag tatagactat
    35221 agtaaacaat atatagagtc tataaatcca gttacaaaag aattaaatat aaaaaatata
    35281 gaagttgttt tgactgatga acaaaagaga ttagaagtat tagaaaaaga actaaatatg
    35341 ttaaaagaag aaaataaaaa tagagattct gagatagtaa atacagcatt tgaagtagga
    35401 aatatcaaat taaacaataa tttataggag gaaattatat gtataactta ttaaagttaa
    35461 tgatagaaca aaaaaactat agcactaagg aggatttaca acataagatg gatgtattct
    35521 atgcagtaaa taggattaca gaagaacaat atttagagtt aacaggttta ttaaataaag
    35581 aagaaacacc agtagaacca acaatatagg ttctttttta ttgaaagaag gtgactaaat
    35641 gacttttaaa gaattagtta gtaaagttag aaatcttgta ttagaagcaa agaatgtaac
    35701 tatagaagat acagagaata aattcactag tgaaaatgtc gagggagcat tgaaagagtg
    35761 tatagataga gcagatttgg cttttcaaag tgccgatagt ggaaaagagt taattgcaac
    35821 tgctatcggc tctcctgcta catccgaaca aacatttcaa gagtatgcta attatattgc
    35881 aggatttaaa aatactataa gtcagttaaa ttctaaatat aaatatacta gtggaacttt
    35941 tacctattat actggtgcat atcctaatgg gctaaatata ggttttagac cagatattat
    36001 ttcctatact gccattaatg ctgtagagga aaaatattat tgtgtataca cacctttatt
    36061 ctatcttttt tataattata caactggaga aaaatatctt gatgttaatc ataatcgtta
    36121 tggaataact tttactgaca atggatggat acctagaaat ccagttaaca atggtgtgta
    36181 tagttatata gcagctaagt gccgttagtt gtcaaagact tagataaatt tctaagtctt
    36241 attttaatac aaaattagga ggaagtatga acgtaacaat agttttttta gcaacaaata
    36301 tatttataaa attagtaata ttagcaatag catttgatac attgctaggt tgtctaagag
    36361 caataaaaac acatcagttt aatagctctt ttggaataaa tggaggaata agaaaagttg
    36421 caatgatagc atgtatattt tttctagcag tagttgacat acttacaaag tttaactttt
    36481 tatttatgtt gccacaagat tgggttgatt tcttacgact aaatcatcta ggaatatctg
    36541 aatttttctc tattttattc attctatatg aaagtgtaag tatattaaaa aatatgtatt
    36601 tatgtggatt accagttcct aaacgattaa aagagaaaat aggtagttta ttagatacta
    36661 tgacagatga attaaatgtt aaaggaggaa aataaataat gaaaatatgt attacagtag
    36721 gacacagtat tttaaaaagt ggagcatgta cttctgctga tggagtagtt aacgagtatc
    36781 aatacaataa atctcttgca ccagtattag cagatatatt tagaaaagaa gggcataagg
    36841 tagatgtaat aatatgccca gaaaagcagt ttaaaactaa gagtgaggaa aagtcttata
    36901 aaatacctag agttaatgct ggaggatatg acttactcat agaactacat ttaaatgcaa
    36961 gtgataggca aggaaaaggc tcagaggttc tatattacag taataaaggt ctagagtatg
    37021 caactagaat atgtaagaaa ctaggtacag tatttaaaaa tagaggagct aaattagata
    37081 aaagtttata tattttaaac agttctaaac ccactgcaat actaattgaa agtttctttt
    37141 gtgataacaa ggaggattat gagaaggcta agaaacttgg atatgaaggt atggctaagt
    37201 taattgtaga aggtgtatta aataagagta ttgaaagtaa tgaggtgaag caaatgtata
    37261 aacacacaat agtgtacagt ggtgatgata gagtatcagc agatatttta ggattatatt
    37321 ataagagagc aaaagaaagt tatctagtta tagatataaa agactataaa ccacatagaa
    37381 cacaaaatct atatgtaatt ggtggagtaa cttgtaataa aatgaaagaa atgagtaaga
    37441 ctacaggaga aaaatttact caactatatg gtaatgatgt atggtcaaca atggataaag
    37501 ttatagaatt tgtaaaagaa aaattataaa ttaaaagaca acctttaaaa attaattgtt
    37561 aataaaacta ataaaatttt caactcagaa ggagcattat atgttaacat ttttagattt
    37621 gtttgctggc attggaggtt ttaggctagc aatggaaaga gcaggacaca aatgcgtagg
    37681 ccattgcgaa tacgataaat ttgcaaactt aagttacaaa gcaatgcata acccaaagga
    37741 ggatgaatgg tttgaaaaag acattagaga aattagagca gaaaatattc ccaaagcaga
    37801 tgtgtggtgt tttggattcc catgtcaaga cattagcatt gcagggaaca aacttggatt
    37861 caaaggaaat cgttcaagtt tatttttctc agttacaaaa cttattagag aacttaaaga
    37921 agaagataga cccaagtact tacttattga gaacgttaaa aatctactta gtgttaatag
    37981 aggatttgac ttcgccaaac ttctcattga gttggatgaa atcggctaca atgcagaatg
    38041 gcaaaatctc aattctaaaa acttcagagt tccccaaaac agagacagaa tatacattgt
    38101 tggacatctt agaagaagaa gtacaagaga agtatttcct atcacaggaa cgagtagaaa
    38161 aactactctt gagcaattaa ataatcctaa tcacagttca aacaggattt ataattcaaa
    38221 tggattatca aagtgtttat taagtaaagg tgggaacaat acaggtcttt atagagttgg
    38281 caatatcaat cctagtggaa atggtgcagg cggacaagtg tatagtagcg aaggcttttc
    38341 tcccacactt acaactggaa aaggtgaaag cccaaagata ttaattaaag agaatactaa
    38401 aaaaggatac atcaaagcat ctataggaga tgggataaga ctagatcata tagaaggtaa
    38461 aactggccgt ggaagagttc agcacaaaaa aagttgtact ttgactactg caagtaatgc
    38521 tggcgtaatt ggtaaaaaat ttagagtaag gaggttgact ccaaaagaat gttttaggtt
    38581 acaaggattt ccagacgatt attacgatag agcggcaagt gtctgttcag aaactcaact
    38641 atacaagcaa gcaggcaata gtgttactgt agatgttgtt gaagaaatag caaagaaatt
    38701 aaaatagaat attaatacaa tgcaaaatgt ggtaggtttt tccttccgca ttttgcattg
    38761 cattagcttt tttggaataa cttacctgta ctatcttaaa taaaaaaatg ctactatatt
    38821 ataaacattt acaaggagtg cataatgtta atacaaagct taaaacgtgg cgaattaatt
    38881 gtaatgaaaa tcttttagaa aagaaattca agaatatcaa agaaagaaat tattcgcatt
    38941 tgcaatgaaa aacacaattg gcggaaatct gcgacaaaaa ctctacttaa aagacttgtt
    39001 aaaaaaagag ttctacttaa gagaattaaa attttccaca tatattacat acctatagtt
    39061 acagaaaaag aatatttcaa atataaaata aatgatttag agccagatta tttagatgaa
    39121 tattttatac gattaatgtc cactgtacat aaatttaaaa atctagatga agaagattaa
    39181 atatctccct tcatctactc ttcacatatt ttaatagact ttcaagcaat aattgactta
    39241 acaaatcatg cttatcataa tgtttattct catctgcaaa tttattaaac atttcccaca
    39301 ctgtattatt tattctaact gtagttcttt ttatagattg atctggaaga tctatagata
    39361 tatcattttc tttaatgaca catatatgat aactatcatc tttagtttta aaccattcca
    39421 gcacattctt tatattatca atttcactag ataaatattt aatatcgtca tcaatcttac
    39481 tgctaatgct atctatatga tatttatcat cataactttt attatcttct acaaacttgt
    39541 ttccagttct tttatatcct ttcttattaa gccacttcct tatagtactt tcatttgccc
    39601 ctaattcatc tccaactttt ttaagacttt caaattcact taatttatta ttaatacaat
    39661 caacaacttc attaatattt ttttctttca aatcactcca agtcatataa atcctcctca
    39721 aaaaaataat agattacaaa tatcatatat ttgtaatcta ttatacatac tataaactaa
    39781 atcctttttt atttcatcat ttttttgtat ccttttgcat ttgcaaaaac atgtttatct
    39841 acatcactca taactattat gccaggtata ttctttttaa atgcttcacc taacaattta
    39901 gcgcctccac ccataacaac aaccgcagct gtactaactg gataatttaa tttcaattca
    39961 ttgtatattt ctttaaatag agaaatagat gcatctatat ttttctttat atcttgtttt
    40021 tcaccattta ggtaaaatcc tttatttatg tagtcttgta ttttttctct ttttatctca
    40081 actttaccta aaaatctttc gtccatctca agagatttct ttattgcatc atatatattt
    40141 attgttccca catttataga atatggatca gtcgctttat tcccttgtac aagcgcaata
    40201 tctgttgtct ttccaccaat atcaactaaa acaacatctc ttcctgcaat ttggtcaact
    40261 atgtcttttg cataatataa gtatcctgta acaccttctg gaaatgtttg aatagatctt
    40321 attgttatat ctcttttagt agtctcattt cctcttgtaa actcaacatg atattctttt
    40381 ccttgtaatt tttcaataag attttttctt aatccggcaa attgtttcac tggcagacct
    40441 agccctagat caacaatttc atcatctgta tttctacata tagcccctaa tagtagtggt
    40501 aagaagtttt ctttctcata tttaactata tttatttcct cgtcaccttc acctaggtaa
    40561 tatgttttat tgcctatttt aatgctatct gaatctgaac caaagttttt tacctctgta
    40621 actttacttt caaaaatatc atctgtactt gttttaactg cataatttcc aatatctata
    40681 cctaatttac tcatttatgt tatttccccc ttattattct aaacttccaa aatcaaaatt
    40741 gttagctgga tcttctattt cttttttctg ttcaactaca gcattatcct ttttttcttg
    40801 cattttttcc caaacaagct cttttaagta agctgtagct gataatttct catctaaaaa
    40861 cttttcaatt gctttttctt tatcattgtt tttaaatgtt agaactatcc tactcattca
    40921 accacttcct tttttataaa atattataaa atttattaat atttatatat attttactaa
    40981 attttacaca gtaattcaat agatttataa gatatttttt ataatatttt ataatttttt
    41041 acgtaatttt ataatttttt ataaaattta tcattttgta tataattaaa aacacacttt
    41101 ttataatttt gcataaaaaa agagtgtttc atatatttct aaacactctt ttctactttt
    41161 tataattatc taattgtaaa agaagcttca gctggactag agaataatat aatacctcct
    41221 gtacacacac cttttccttt aaatttagca atatatgaac ccttttttag ccctttaaaa
    41281 gttactacac cagttttagt agttccagct aatctaaagc ctacatactg agatgataca
    41341 attttgccag ccttatatac atctatattt ccttcaaatc ctaaaatatt tccaagcgta
    41401 gatgttactc tccaagataa ctgcccacca ctcattgcag ttaaattagc agtaccacct
    41461 ggaacaacaa cagatttagt cgaaggcgaa tctgagtttg gttggccaat taattcaact
    41521 gtttgtgaat ttccagtacc ttcataatca tttccatcag caaaagctgt aaatggaatc
    41581 attactgcgc aaagcgttag taataaagca attgtttttt tcatttgttt tccccctatt
    41641 tttttatatt attctcaatt acagtttcct ttctttacgt gccttaataa tcatattgta
    41701 attatgctca gcagtttcaa atgctcctac caaaggagtt ataattacac tttctattaa
    41761 gcacaattca tcatagttta aactagcttc tccagcatag aaattattta acttatctaa
    41821 atctattcta gttaaaacgg aaactgattc cattgagaat ttgtaaacat caataagttt
    41881 atataatcca cctatatagt tttttgctac ttcatggctc ttaaaaaatt catcttttcc
    41941 tgtatgtagg tttacttcca taattccctc cattgttctt atatttttaa ccttcaaata
    42001 atatttactt cttcttcaaa acagaatatt ctatcacggt agaatattct gtttttttca
    42061 ttctatcatg atagaataat attttcaatc attttttcta ttctatttta tttaaataga
    42121 atagaaaaaa agaacactta atatttttca agctaatata ttaagtgttc tttttatatt
    42181 tttacaagat tatataaaat tattctttaa tcaattaaag tgtttttcta tttcttaata
    42241 ataagaaatg atataaaatt gtatcatttt aaagattttg tgatataata aaagcaagaa
    42301 gaactacaat ctatttaggc gtagagtgga gttcataata aacgtagttt attttttgaa
    42361 tttaaatata aatttaaatt caacctgtaa gtcactcttt gcacgagagt ggctttttgc
    42421 ttttttgaat agttcactga ttaagtaaac taccacacta gctgttaaac tagctaaaac
    42481 attaaacaaa aagttatcca tgtaactcac ctcccctcga atcgttggga ggataatctt
    42541 ttgtacatga actccactct atagattgta tattacgctc ttcttgctaa atttaattat
    42601 accatatttt gtaatatcag ttaagagttt gcactacaat aatcagaaag cttgttccac
    42661 tcacaatcaa cccctattat tttaagaata tcattgactt ggctatttaa atctttaata
    42721 tctgatttag acattaaagg atctttcatt atttcattta gaaaaatact atcaatgatt
    42781 aaacatacat tttctgataa atattcacgt tttactgaga ttactgttcc aactgtactt
    42841 tcttttagtg tcttatatgc tacactcaat atgcatggtt ctaaattaat tccttctagt
    42901 actgtttcaa agtaatattt tattcttata ttttcatttc caataaatat ttcttcattg
    42961 ctgcttatct tctttaattt tctcattatg cctaacatct tagcattcac ttcatagttt
    43021 attccacatc tctttgatgc taaactcgac atcaagtctc tttctgaata aattacccat
    43081 tgaccattat aggaaaatga tgttttagcg atatccattt ctccttttcc agtaatatta
    43141 aatttaggct ctagtttatt tattaagtaa atctcataaa aatccatagt atatttatta
    43201 tctaattcta tatactcaaa tcttaaattc ttattacacc aattttcttt tttattactt
    43261 aaatgactag cgtgtctatc gcagatattc tctgttttcc caacgtataa acattcttcc
    43321 gtatctagat taatatattt ataaacataa tacatttttt attttctcca attattattt
    43381 atagtaatat ccttcttcat ttactagacc ttgatcaaat aaatttctcc taacagtttc
    43441 aagtctgaca ggatctttgc tataatcaac ttctctttct tcttttttaa catcttcatc
    43501 atcccaatac ttatccaatt cttctttaaa ctttaaatca tctttttgat gaattataac
    43561 ttcacttatt ttatttgaaa gtgttgattt aaaaaaatca taattctttg tatttataat
    43621 atcagtatta tctctttcaa atgctatagc aatagactta ttaaaagcat tcagataaac
    43681 atctttctta aaatcaaact ctttaaaatc ttctttaaat agtttaatcg tccctgttgt
    43741 aaaaatattt tcatttggta tataaaaact aaaagacact tcattgtcct gcttcttttt
    43801 aggtatataa tcttcaacca atatatcttt atcaaaatat tttctaggtt cataatctat
    43861 cacagaaaaa attatttcat ctatttttct attttttctt ttttcttctt ttattgaaac
    43921 aaccatgttg ccttttttat ttatttcatt cattgctctt tttagaacat tttgtttaaa
    43981 atatttatac tctggatata cattatcttt taatttcaaa taaaatctta attcatctaa
    44041 cttatatttt atctccactt ccttattttc tctgctccat agtctaaata aagtgtataa
    44101 cctctgagta tatgcacctc taaaattaaa taacactgat aaattaatag ctgtatatcc
    44161 atttttcttt ttctgcattt ttacaaaatc agttatatga ttgtatagaa cttcatgcat
    44221 cataattgta taaatttggt ctgtatgatc caattcatat gtattaatta aaccacttcc
    44281 aaaagttttt aacttgccag ttgtctcttc tatatagtca aattccaaaa cactttgttg
    44341 aaacatgttc aaaatttctt ttatattttt atgctcataa tcattattgt ttttcataaa
    44401 tacttttagt tcttcttttg atatagttgt aacataagat gaattttgct gtttctgagc
    44461 attaaaaagt attttgtaaa ataatttatt ttccacattt gtaaaatcat atttactttt
    44521 tattaaatta tttggttgca ttaaaatttc ttttttttcc aaaatctatc acctcatata
    44581 aattataata cctaatttat aaaaaggtca accatttttg aaagacgctc acctttgacc
    44641 cttaaaatga tgacctttga cccttaaaat gatgaccttt gacccttaaa atgatgacct
    44701 ttcatctctg aaacctagat ataccaatta ttatagcttg tctaaaaact attaaagact
    44761 ttaaaaactg ttttaaaaac tattaaagac tagaaaagat gttctgaact attctttttt
    44821 tatattaaaa aactttagta tttatttttc tagtatactt taaacactta ctaaaaataa
    44881 tttcttataa aagattggag atgaagaatt ttttatgctt gaaaacagaa aacaaattca
    44941 gaaagaaatt tacttaccaa tagcaacata ttgtttaaaa gataagcaat attatataaa
    45001 agaatatgga gcactattaa taaagagtgc tgaaaatagt gacattttta atacccataa
    45061 aaatagaatt ttgtataaga ataaacttga tattcaagaa atagcaaatg aactgaatgt
    45121 aaataaagca acaatccaaa gaaatataaa aaaactagag aagttagatt ttaaaattct
    45181 aaaaatagaa aataccctaa atggaattgt atattgttta ccatcagaaa ataatattga
    45241 tttaaacaaa tttgcattga taaattatga aatgcttaaa aaaatggtca ataaatttaa
    45301 atcaaataca ataaaagtat attttttact taaaacaatc actacagaaa ctaattttaa
    45361 acctgcaact aatagtttta tagcagaaaa tataggacta agttctaaaa gtaaaaataa
    45421 tttagatatc ataacttctt ctgttaaaac tttagaagaa aataaataca ttgaaacaaa
    45481 aaaagtaaat gtatatgaat atgacaaaga aaaacttaga gaagttccaa aaataagaaa
    45541 agtttatcgg atttgtaatt ttgatgagtg gaaaaaggaa attgataaaa ataaagttta
    45601 ttaaatatag cttaaaagca tttaaaggct tgtctgacgt gttttaatta caaacttgat
    45661 gttatttacc taaaaagata caaatttgct taaaaatgga aatagaaggt caaatatata
    45721 aatagagaac caaataaaat tatattggct ctctatttat cttcttccca atcctcaata
    45781 aacttttcta aactttttaa tttattttca gacacttctt catcatcatg cagagcactt
    45841 ataaaacttt caaaagaatt tttgtgaaag aaactaaaga aacttttagt ctcaactttt
    45901 aaatattctt ctttttctat taatgcttta taatatgtaa atctatcttt cttaacaacc
    45961 tctaaaaatc ctctaccaac taatctcctc aagaaagtta acatagttga ttttttccat
    46021 tcgtactttt cgcctagtgt cttcaaaatt tcagtagatg caacttcttt atcttcgctg
    46081 tcccaaataa acatcataac caacaattca ctttctggaa tcttttttat cttcataaag
    46141 tacaccacct tctaaaagta tatctaaaat actataacaa tagagttaac tacacaatac
    46201 taaatgtcta aaaatgtttt accaattttt accaattaat ttttcttt
//
LOCUS       DAYAPA010000016        45786 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.016, whole genome shotgun sequence.
ACCESSION   DAYAPA010000016 DAYAPA010000000
VERSION     DAYAPA010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 45786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45786
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.016"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            45..1112
                     /locus_tag="ACGI59_000826"
     CDS             45..1112
                     /locus_tag="ACGI59_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HFG9738632.1"
                     /translation="MKNKLVYICIMSLLLFVVGCSNNPNKESTIKKDSKNNQSQVKEE
                     KREPVKKDITISFAGDVTMGNYKGSSYYGSFDHEFKRQGEDYNYFLKNVKPVFEKDDL
                     TVVNLEGPLTTASNAKEKKFAFKGKPGYVNILKSGSVEAVSIANNHSEDYFEEGMKDT
                     QFILDENKINYFGLGKDAVVDVKGIKVGLLGYNGLSTEYNEKNLKQMEDDIKSLKKKS
                     DVVVVYFHWGIELDYYPDKKQKDFAHYAIDKGADLVMGSHPHVIQGIEKYKNKYIAYS
                     LGNFCFGGNKNPSDTDSYIYQQTFTFSDNKLTSIKEPNIIPTSITSSNSRNNYQPKIL
                     DGSEKDRVLNKLEKISKDLNK"
     gene            1282..3183
                     /gene="cooS"
                     /locus_tag="ACGI59_000827"
     CDS             1282..3183
                     /gene="cooS"
                     /locus_tag="ACGI59_000827"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_function: GO:0043885 - anaerobic carbon-monoxide
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006091 - generation of precursor
                     metabolites and energy [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic carbon-monoxide dehydrogenase
                     catalytic subunit"
                     /protein_id="HFG9738633.1"
                     /translation="MACNTCKMCESADKKLESFVASKDVETAFHRTEDQKVKCGFGLQ
                     GVCCRLCSNGPCRVTPKSPRGICGADADTIVARNFLRAVASGAACYLHVVENTAKNLK
                     NVGITKGVVKGEKTLNQLAEMFGIESDEKYDKCIKVADKVINDLYKSRDDKMELVEKI
                     AYAPRVKKWKELGIMPGGAKSEVFDAIVKSSTNLNSDPVDMLVNCLNLGISTGLYGLT
                     LTNLLNDVMLGEPVIRMAPVGFNVIDPDYINIMITGHQHSTFANFQDRLKDEDVIKLA
                     QSVGAKGFKLVGCTCVGQDLQLRGEHYQEVFAGHAGNNFTSEAVLSTGAIDIVLSEFN
                     CTIPGLEPIADKYKVKMVCLDDVAKKANAEYIGLDRSKLDELSNTLIEKALESYKERR
                     GSIEIDIPKDHGFEQSLTGVSEKNLKAFLGDSWKPLINLIAEGKIKGVAAVVGCSNMT
                     AGGHDVNTVELTKELIKKDIIVLSAGCSTGGLENVGLMSPGAEELAGENLKEVCKTLG
                     IPPVLNFGPCLAIGRLEIVATELAAELGIDLPQLPLVLSAPQWLEEQALADGAFGLAL
                     GLPLHLALPPFITGGKLVTEVLTEKLKDLTGGHVIVNPDPKSSANQLEEIIIDRRKNL
                     GLKDVECNA"
     gene            3176..3649
                     /locus_tag="ACGI59_000828"
     CDS             3176..3649
                     /locus_tag="ACGI59_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFG9738634.1"
                     /translation="MHRIFINKDLCTGCKSCVLACMLKHNKDYDMYTLDLENIDNDSR
                     GHIELDSKHNNPVPILCRHCDEPECVLACMSGAMHKDSESGIVSYDEEKCGSCYMCVM
                     SCPYGLLKPDDRSKQNILKCDLCKDEEYPRCVANCPSGAIELQKEENDELCSVRG"
     gene            3624..4850
                     /locus_tag="ACGI59_000829"
     CDS             3624..4850
                     /locus_tag="ACGI59_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFG9738635.1"
                     /translation="MNYVVLGASAAGINAVKTLRELDKDSNIVVISKDENVYSRCMLH
                     HVISEHRILKQINFVDEDFMEQNNVKWIAGKTVKGIDINKKVVQTEDITVNYDKLLIA
                     TGASSAIPPIKNLRDGNFVYSVRNIDDIYKIKEKAQNSKNVVIIGAGLVGIDALVGLF
                     KYEQLNISVAFMEKYILDRQLDEYTASVYENKFKEKGVKFYPSASIQEIVLDNSKNVT
                     GVAFSNGEVLDADMVIVATGVKPNADFLDGTGIEYDRGIIIDDMCQTTQKDIYAAGDV
                     VGKNAIWPLAVKQGIVAAYNMVGKDKKIEDEFAFKNSMNFMDIPTISIGMNTPVDDSY
                     KVLTRHGINDYKKFVFKDNVIYGAVIQGDISYVGVLTYLIKNKVEIYDLENRIFDICY
                     ADFFNIKENGEFCYSV"
     gene            complement(4989..6176)
                     /locus_tag="ACGI59_000830"
     CDS             complement(4989..6176)
                     /locus_tag="ACGI59_000830"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFG9738636.1"
                     /translation="MNITKNFLKDYLEKFGETPFLVRLKDEEEFLIGEGSPQFKVIVN
                     NDISKSELLKSTSLALGEAYINRDIEVEGDLFLTLNLLLKQIDKFNIDNSAVKHLLHS
                     SNSMRHQKKEIHAHYDIGNDFYSLWLDKTLSYSCAYFRNDNDTLYDAQVNKVHHILTK
                     LNLEEGMSLLDIGCGWGFLLIEAAKKYKIHGVGITLSEEQFKEFNARIKEENLQDYLE
                     VRLMDYRELKKSDLMFDRIVSVGMLEHVGRENYELFIKNVNAVLKDGGLFLLHYISGL
                     QESYGDPWIKKYIFPGGVIPSLREMIHICSDYKYHTIDVESLRLHYVKTLLYWQKNFS
                     ENLPKIKEMFDDKFIRMWDLYLCSCAAAFNTGIIDIHQILFTKGIKNDLPLTREYMYN
                     CSK"
     gene            6449..7273
                     /locus_tag="ACGI59_000831"
     CDS             6449..7273
                     /locus_tag="ACGI59_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFG9738637.1"
                     /translation="MEVYALVGSSGTGKSFKALEFAYENDIEYIIDDGILIYKNKVLA
                     GISAKQANTTIEAVKRAIFYNLEHRQEVREAIKKENIRKILIIGTSKKMVNQIVERLS
                     IGRVCKFINIQDISTKYEIEIAKQARKEGNHVIPVPAVEIKSMASGLSINSLKRLFRK
                     GSNRNMVVLEKTIIRPTFSYIGKFYISADVIKQIIEYEVYNFGSIDRINKINIENCNN
                     FMNIFVSININDLETIKKIEDIQQTIKKSIEKMTLVNVQKIDINIHKIKKINFLQC"
     gene            7402..8667
                     /gene="gluD"
                     /locus_tag="ACGI59_000832"
     CDS             7402..8667
                     /gene="gluD"
                     /locus_tag="ACGI59_000832"
                     /EC_number="1.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-specific glutamate dehydrogenase"
                     /protein_id="HFG9738638.1"
                     /translation="MSGKDVNVFEMAQSQVKNACDKLGMEPAVYELLKEPMRVIEVSI
                     PVKMDDGSIKTFKGFRSQHNDAVGPTKGGIRFHQNVSRDEVKALSIWMTFKCSVTGIP
                     YGGGKGGIIVDPSTLSQGELERLSRGYIDGIYKLIGEKVDVPAPDVNTNGQIMSWMVD
                     EYNKLTGQSSIGVITGKPVEFGGSLGRTAATGFGVAVTAREAAAKLGIDMKKAKIAVQ
                     GIGNVGSYTVLNCEKLGGTVVAMAEWCKSEGSYAIYNENGLDGQAMLDYMKEHGNLLN
                     FPGAKRISLEEFWASDVDIVIPAALENSITKEVAESIKAKLVCEAANGPTTPEADEVF
                     AERGIVLTPDILTNAGGVTVSYFEWVQNLYGYYWSEEEVEQKEEIAMVKAFESIWKIK
                     EEYNVTMREAAYMHSIKKVAEAMKLRGWY"
     gene            8880..9470
                     /locus_tag="ACGI59_000833"
     CDS             8880..9470
                     /locus_tag="ACGI59_000833"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HFG9738639.1"
                     /translation="MHFKSKDLNLKYLVLFLKIVYWFNPFIYIMEKYMDLDCELYCDE
                     RVLKNCTVQQKQEYALTIVNSMKRSTNFSNKFVAGLNKQSDIRKRVINMFSEKHKNGI
                     LRALIISLFSSITFFKFDSTSIIEENDFLERSRYTFEGRQEDMFVTIDFIYADVPIKL
                     RKEHEENCRVNGIVPKDSDSIEIIPRFYKDCMDLKK"
     gene            9801..10934
                     /locus_tag="ACGI59_000834"
     CDS             9801..10934
                     /locus_tag="ACGI59_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HFG9738640.1"
                     /translation="MSNFILNGIFRTVIVSSLSILMILIFKKNVFKRFSKKFNYYIWM
                     IVVIKLFLPFTYYTFTINILRSKKHININNINLEGFNNVSTISNNIILYTWIITVVVY
                     LIYTIFKYIKLKNLINDLSYDVDDGEIVNLYKNILEEFNITKDIKLKYSYEVETPAFF
                     NSCVLLPPHEYKLKELDWIFRHELMHFKSRDLYLKYVILFLKTVYWFNPFIYIMDKHI
                     DLDCELYCDERVLKNRNSEEKKDYALTILNAMRKGSNTSNKFIAGLHKQSDIKKRVMN
                     MFNEKYKNGILMALTLCLLSSITFLKVNSTSIVNLNILPKGMEEKDVTNSAIVTMIEF
                     TYAEAPKSMREEHKINCEFLGLIPKDSDKIGVGIEYYDSLLND"
     gene            11850..12872
                     /locus_tag="ACGI59_000835"
     CDS             11850..12872
                     /locus_tag="ACGI59_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFG9738641.1"
                     /translation="MKKKILIPVFASVMALSVSSIVNADEVNDSSQNKDDKTNTELNL
                     GEYKEVKYKVAKIKDGVAIKIREEGQVQNIAYSGDEFTVLGTQGEWVKVKVEDGEGWL
                     ATRYVDISEGVGYTNADKVNLRKDKSESSEVIEELEKGSSLLVLEDNGDWLKVKDGET
                     EGYVKSSYISDKAPVIEEPQVNPSIDQNVDSTNNNQQNNANTNQQNNNSNVPTANSNA
                     VQAVLNLAYSKQGCPYVWGAEGPNTFDCSGFTQYVYRNAVGKNIPRTSKAQSKYGQTV
                     SKANLQPGDLVFFTTNGSGSVSHVGIYVGGGNMIHSPSTGKTVSVTSINSSYYTARFV
                     TAKRIL"
     gene            13168..14088
                     /gene="pyrB"
                     /locus_tag="ACGI59_000836"
     CDS             13168..14088
                     /gene="pyrB"
                     /locus_tag="ACGI59_000836"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HFG9738642.1"
                     /translation="MLKSRNLIQPEDFSIEEIDEILELAQKIIDNPSKYSRICEGKLL
                     ATLFYEPSTRTRLSFESAMNRLGGRVVGFSEPNSSSASKGETLGDTMRIVSGYVDIIA
                     MRHPQSGAASEAARYTEVPFINAGDGKNQHPTQTLTDLLTIKSLKGTLESHTIGLCGD
                     LKYGRTVHSLVKAMARYKNTKFVFIAPEELKMPDYIKEAIKGHAYYETNNLDDVIGSL
                     DVLYMTRVQQERFEDKSEYERLKNYYILNKAKLEKASKDMLVMHPLPRVNEIDIDVDS
                     DDRAVYFKQAKYGMYVRMALIIKLLGINED"
     gene            14127..14819
                     /locus_tag="ACGI59_000837"
     CDS             14127..14819
                     /locus_tag="ACGI59_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HFG9738643.1"
                     /translation="MYKILENIYIGEDMYRMKVKGNFEGKMGQFYMLRAWDTYPVLSR
                     PISIHDIDEEGITFLYKVVGEGTQILSNLKVNDNIKLEGPYGNGYAKVDGKVALVGGG
                     IGVAPLYLVAKNIKNCDAYLGFREDVILEDEYKQVCNKVYTTVGNTFVTDIIDVEKYD
                     YILTCGPTPMMEKLVKMVEGTKTRIMVSLENHMACGVGACLVCTCKTNGGNKKTCKDG
                     PVFWGEDVIFNG"
     gene            14812..15714
                     /locus_tag="ACGI59_000838"
     CDS             14812..15714
                     /locus_tag="ACGI59_000838"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HFG9738644.1"
                     /translation="MGNLSVKFGSVEFKNPVIMASGTFGFGKEYNEIYDIQKLGGISS
                     KGLTLNKKPGNNGMRVHETSSGMMNSVGLENPGVQGFIDYELPFFSKLDLVRIANVGG
                     GTLEDYLLGVQMLNDKPIDIIELNISCPNVKAGGMAFGIKNEVAREVVREVRNITKLP
                     LVIKLSPNAEDIVGMAKVCEEEGADGVSLVNTFKAMAIDIKNRRPVFENVYAGLSGPA
                     IKPIALRMVHEVCKNVNIPVMGMGGITKATDAIEFIMAGATCIQVGTANFMNPRIGIE
                     IIDGINEFMDREGIKSLDEIRGII"
     gene            15799..16383
                     /gene="pyrE"
                     /locus_tag="ACGI59_000839"
     CDS             15799..16383
                     /gene="pyrE"
                     /locus_tag="ACGI59_000839"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFG9738645.1"
                     /translation="MSKVDVVDILKKSDALLEGHFLLSSGKHSNRYVQCAKVLRFPEY
                     AAEVLSTVVEQIKDLDIDLVVGPAMGGVIVSYELGRQLGKEAVFTERKDNTMELRRGF
                     EVKKGAKIIIAEDVVTTGKSTMETKRVLEALGGEVVGVACIADRTNHDIGMPIYSAIK
                     LDIQVYESDECPLCKEGKLPVVKPGSREFKELGM"
     gene            16597..17523
                     /locus_tag="ACGI59_000840"
     CDS             16597..17523
                     /locus_tag="ACGI59_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HFG9738646.1"
                     /translation="MKNQLNENHKKVVFFGVGAVGATFAEQFFNSKYDFKILCDNERK
                     KRYLEEGFIINGKRYDFDYVTKDEYKQEADFIIIGLKYNNLKENIKELDGLVGKNTVI
                     MSLLNGVDSEEIIGERFGIEKMVYSYVTNIDAKKINNNIIHTTNGIIVFGNKDNSEDR
                     KTNIITEVFDDVNIEYTLSKDIQRDMWWKYMVNIGVNQTSAILGAPYGVFQSSEHLRE
                     LAKSAMREVVAIAQAKDISLTEDDVEHSLHRILEHSKEGRTSMLQDVEAHRLTEVDMF
                     SKNICKLGKKYNIPTPINQTFFYMIKVIESRF"
     gene            complement(17575..18465)
                     /locus_tag="ACGI59_000841"
     CDS             complement(17575..18465)
                     /locus_tag="ACGI59_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFG9738647.1"
                     /translation="MFEELKTFVAVVEYKNFTKAGEYLNLSQPSVSKHIKNLENYFKV
                     VLINRSIKQKTIFITESGQILYKRAKEILNLLNITYHDVSQVSDAITGHLKIGASLTI
                     GEYILPNFLALFSKKYPDIDVEVFIKNTSIVSSHVKDYILDIGLIEGTCSSPSFIQEY
                     FFEDKMVLALPYKSHLLKDFSFDKLQNQKWIVREDGSGTRDYLDMFLSVKEIIPKSMM
                     VFGSNYAVKESVRNNLGITIVSNLVTSLPVLNNELSVIELGSSYNRHFSYIFPKDITL
                     SKAATIFIEELKIFSNLNSI"
     gene            18653..19684
                     /locus_tag="ACGI59_000842"
     CDS             18653..19684
                     /locus_tag="ACGI59_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HFG9738648.1"
                     /translation="MLKKKSSLTLKTIREILPGLFVSVLVGYISIFISILIPKVGAAS
                     ISIFLGMFVGNLFLNQKVFQKGYKFSETDLLSYSIVLLGATLSVSTLIDLKVSGILFI
                     ILQMTITIIAALYIGKKLGFEENFRFLMASGNAVCGSSAIGATVPVVNATDKEKGIAV
                     TIVNVTGIFLMFLLPIISQFLYSHELVKTSAMIGGTLQSIGQVVASGAIVGENVKDLA
                     TIFKIVRVIFLVVVVLVFGHMKNQSNREILEEEKDEIKKKKVTIPWYVIGFFITCALF
                     SMNIIPKEVSVLCKEISNKLEIIALAAIGLKVNVKDLVKQGKEVSLYGLFVGIVQVVS
                     AVVLIKIFI"
     gene            19978..20853
                     /locus_tag="ACGI59_000843"
     CDS             19978..20853
                     /locus_tag="ACGI59_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA glycosylase"
                     /protein_id="HFG9738649.1"
                     /translation="MNVYEKGNGVILEGVTDFDPVHIFECGQCFRWHKQEDGSYTGVA
                     KGRILNVKKENDKIYLNNTNLKEFNSIWYNYFDLGTDYTEIKNKLKNMDEYLNKATEF
                     GWGIRILRQDGWEMLISFIISSNNRIPMIQRAIENLSRKFGKYIGEYEGNEYYAFPTP
                     EELNKASQEEIRACQTGFRDKYIKSTTQAVIENNDEVSEYTNLSTEDCRKELLKFNGV
                     GPKVCDCIALFGMQKYDSFPVDVWVKRVMQEFYIDEDMSLPKMRTYGIDKFKEMSGFA
                     QQYLFYYARELGIGK"
     gene            20871..22361
                     /gene="cls"
                     /locus_tag="ACGI59_000844"
     CDS             20871..22361
                     /gene="cls"
                     /locus_tag="ACGI59_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFG9738650.1"
                     /translation="MGVIGTIFLFYLIISYLAGAIISVIILLENRDPAKTMSWLLMFI
                     IFPGVGLMIYAISGRNIRKRKLFKTQKLANNIKEKKLFDTLEKITEIVELEKESIKQN
                     KLLRDEEDGSYRKRVINMLLKTGMFPFTKNNKVDVFVDGNEKFKRLIEDIREAKDHIH
                     LEYFIIKDSEIGRVLKEELIKKAKEGIKIRILYDDVGCWRFWFNRKFFREMREVGIEI
                     AAFLPTKFPIIGGKLNYRNHRKIVVIDGIIGYTGGINIGDEYLGKNDKFGYWRDTHIR
                     IKGISVYMLQMTFLIDWYYTTKEVLVTKNYFPSVGNVGESMIQVVASGPDSDWEDIHY
                     AYFSAICQARKNVYIETPYFIPDESLLKAIKSAALSGVDVRIIFPKIADHKIVNIASY
                     SYFEEILRAGGKVYLYNKGFIHSKVVIIDDKIASAGTANMDLRSFMLNFEVNAFIYDE
                     EVIRVMTDDFFEDLSHCEELNLEVFKNRNIIQKIKESVARLFSPIL"
     gene            22554..22838
                     /gene="groES"
                     /locus_tag="ACGI59_000845"
     CDS             22554..22838
                     /gene="groES"
                     /locus_tag="ACGI59_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFG9738651.1"
                     /translation="MKIRPLADRVVIKKVEAEEKTASGIVLPGAAKEQPQIAEVVEVG
                     PGGIVEGKEIKMELTVGDKVIFQKYSGTEVKIEGQEYTILRQSDVLAVIE"
     gene            22871..24499
                     /gene="groL"
                     /locus_tag="ACGI59_000846"
     CDS             22871..24499
                     /gene="groL"
                     /locus_tag="ACGI59_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729227.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFG9738652.1"
                     /translation="MAKEIKFSEETRRALEAGVNKLADTVKVTLGPKGRNVILDKKFG
                     SPLITNDGVTIAKEIELEDRFENMGAQLVKEVATKTNDVAGDGTTTATVLAQAIIREG
                     LKNVTAGANPILLRKGIQKAVTVAVEELKNQSRIVETQEAISQVASISAGDEEVGKLI
                     AEAMEIVGKDGVITVEESQTMNTELDAVEGMQFDRGFVSAYMVTDVDKMEAVLNDPYI
                     LITDKKISNIQELLPVLEQIVQQGKKLLIIAEDVEGEALSTLVVNKLRGTFDVVAVKA
                     PGFGDRRKEMLQDIAILTGAQVISEELGYDLKEADLSMLGRASSVKVTKESTTIVDGS
                     GDKKAIEDRVTQIKHQVEQTTSDFDREKLMERLAKLAGGVAVVKVGAATEVELKERKL
                     RIEDALNATRAAVEEGIVAGGGTAFVSVIPAIGTLIESLEGEVKLGAQIVKKALEEPL
                     RQIAINAGLEGAVIVQNVVNSEAETGFDALNEKYVNMIEAGIVDPTKVSRSALQNAAS
                     IASTFLTTESAVADLPEKEDAGMPGMGGGMPGMM"
     gene            24761..25501
                     /gene="larB"
                     /locus_tag="ACGI59_000847"
     CDS             24761..25501
                     /gene="larB"
                     /locus_tag="ACGI59_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HFG9738653.1"
                     /translation="MDLRKLLEEVKNENIDIDFALEKLKDLPYEDLGYANIDHHRELR
                     NGYPEVIYCEGKSDEHILGIIDKMNQKSSNILGTRCRKETFLKIKEIYNHAEYEEASK
                     ILKIQNHDIENIGKGKIVIATGGTSDIPVADEAYHTAKFLGNDVDRIYDVGVAGIHRL
                     LNKRHKIDSARVIVAVAGMEGALASVIGGLVDVPVIAVPTSVGYGANFGGLAALLAML
                     NSCASGISVVNIDNGFGAGYLASMINKL"
     gene            25636..26768
                     /gene="larC"
                     /locus_tag="ACGI59_000848"
                     /pseudo
     CDS             25636..26768
                     /gene="larC"
                     /locus_tag="ACGI59_000848"
                     /inference="COORDINATES: protein motif:HMM:NF014072.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
     regulatory      26828..26929
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            27150..28685
                     /gene="guaA"
                     /locus_tag="ACGI59_000849"
     CDS             27150..28685
                     /gene="guaA"
                     /locus_tag="ACGI59_000849"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFG9738654.1"
                     /translation="MKHELVLVIDFGGQYNQLIARRVRENNVYCEILPCTASIERIKE
                     KNPKGIIFTGGPNSAYLEDSPTISKEIFELGVPILGICYGIQIMSHVLGGVVRKGNKQ
                     EKEYGKTAITYGKSSLFEGITTNSVWMSHTDLIEKVPEGFTIVANTNDCPVAAMENVE
                     RNLYGVQFHPEVEHCLEGDKILTNFLYNICKVKGDWTTDSFIEDKIKELKEKIGDKKA
                     LCALSGGVDSSVAAVLIHKAIGDNLTCIFVDHGLLRKNEGNDVERIFREKFDINLIRV
                     NAEDRFLSKLKGVSEPEAKRKIIGEEFIRVFEEESNKLGKMDFLVQGTIYPDVIESGH
                     GNAATIKSHHNVGGIPEDVDFQEIVEPLRELFKDEVRKIGLELGIEEGLIFRHPFPGP
                     GLGIRVIGDVTKEKCDILREADAVYMDELRKAGLYREIWQAFATLPDVKTVGVMGDER
                     TYAYLVGLRAVTSSDGMTSDWYKMPYDVLERISNRIINEVDGVNRVVYDITSKPPGTI
                     EWE"
     gene            29263..30654
                     /locus_tag="ACGI59_000850"
     CDS             29263..30654
                     /locus_tag="ACGI59_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFG9738655.1"
                     /translation="MRNKIIIGITFLLVLLMSGCASKQTAEINVLATTDLHGVIPYEL
                     TSYVKEERKKDKNLTLVDAGDFFDSGEVFGSSMDSYFSARYKDGDNNTESYIETPISK
                     DMKEVGYDVAVLGNHEFISNNKFYLDNMISDFEKQKINILSANTYKKNGDSYTKPYII
                     KNINTPEGNVKLGILGLTIKEVGERKQWKDGKLVDSKSLELKDQEGYNGELYMNDLVE
                     DAKKWVSIMEKEKPDIIVAVAHTGEKPKKPKNPGNRIQDLAQQVDGIDAIVAGHNHVQ
                     IEQHDYKNKSGENVIVTEPGKHGECISKINFKLEKNNDKWNIVDKSSKLIKFEKNQES
                     DKFGEFISSINKVSKDKKEIRLSEVKTPEWDKIYVFKSGTSADDIYKTIGYKWRNISS
                     PKSDGMVQIVVMNNNKPVSYIYGEAEVMPINIKCDESEYKDNVVTIYPNKNDKFKIEK
                     GKQEYSINLKHIQ"
     gene            complement(30802..31110)
                     /locus_tag="ACGI59_000851"
     CDS             complement(30802..31110)
                     /locus_tag="ACGI59_000851"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFG9738656.1"
                     /translation="MLREQGSAIRDTLDSVLSLSNQKAYPVWESVNSFALIKAAQAGL
                     GITILPENLLLDSLLHKKLRLIELDGIDMENKMLAILHKDKNITQPLKIILDNISNVL
                     "
     gene            31147..31668
                     /locus_tag="ACGI59_000852"
     CDS             31147..31668
                     /locus_tag="ACGI59_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HFG9738657.1"
                     /translation="MFSIGGGYAIIPLIQEKVVNQTGWISEKIFTDIITISQMTPGPL
                     AVNTSTFVGLQIGGFWGAVAATLGCVLCGVVISLFLYNFFQKHQNSTYIFEILNGLKS
                     ASLGLIISAAATIIILALFGTNKVYFNSIFEMLNWTALIIFFVTLLIVRKWKINPIVI
                     IIMSGILGIFFYL"
     gene            complement(31797..32033)
                     /locus_tag="ACGI59_000853"
     CDS             complement(31797..32033)
                     /locus_tag="ACGI59_000853"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9738658.1"
                     /translation="MLECFIAKDSKIKDGVNIPTLVFKTLHDRYFKTQYFDSYYNSLK
                     CKKKLVEINNVHNSYYIDSKKFCQEAYDWFVENQ"
     gene            complement(32343..35771)
                     /gene="uvrA"
                     /locus_tag="ACGI59_000854"
     CDS             complement(32343..35771)
                     /gene="uvrA"
                     /locus_tag="ACGI59_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFG9738659.1"
                     /translation="MKINNYKNQSIITNPKKFENKYQDLPKTPIELLKVVQSLVIHGD
                     QGKLYGISFNKQQSDEELLRTIPQMLKRIFEINSNPLTIPRNPKQRLVGMCRDYSLLL
                     VSLLRYRGFEARMRAGFANYFESELTYEDHWLVEYYDTLTKRWIRIDAQIDDIQKNYF
                     QINFDTHDVGKTDGFLTGSEAWIRCRQGHAHPDDFGYNKNWKGWHSVKGNLLHDFNNM
                     IGLELLPWDLWTELSSKKYNQLTRAEKNLLDEMAEILSSGNIKIEALNLLIEKLPEDY
                     LKSIFSQLKILGISEIKELGNPLELEKKFKFTKAINKSIKNSLCHNKSSIYLKGGRQN
                     NLKDVEVTIPKNQITVITGVSGSGKSSLAFDTIYEEGKRRYFENLSNGAKLSEQLQKP
                     EFDLLQGLTPTIAIEQKKGSQNPRSTVGTLTSIWDYLRMLFVSIGKSYCPYCKIPLEK
                     KNNTKNYCPHCQTIFSKINTSTFNANSHTGACHDCNGLGFTYQVNPQLIVKDPTISIL
                     DGATYYFGKLRGKKPNGNWMVGELYAIAKDKNIDLDIPWNELPRDFIDAILYGTDDKI
                     YEFSFESKGRESKIRRPASGAINHIQRLFRESSSENNTLHQYMNKIPCNTCGGELLCI
                     EARFTTIKGYRFPELTKMTIEQLWNWLCELPNHLQKNELSLVNDILTELKIRVSYLLK
                     VGLSYISTDRTAPTLSGGELQRVRLSSQLGSELVGLTYILDEPSIGLHPRDHNLIIKM
                     IEELRDKGNTVIVVEHDKDTILSADYIIDVGPSAGTKGGFIIAEGTTQEIIKNPNSIT
                     GKYLSTYNKISSQNKIIPSKWLSLKGCHANNLKNIDVEIPLNCMCSITGVSGSGKSSL
                     VFHSLLPALEEKLKQKSIPDKNYTEFTGFDAIDDFILMDQAPIGKSSRSTPATYINIF
                     DEIRSLFAETPQAKQKLLDESYFSFNSKKGQCPNCQGLGKTKIILQYMADQWVTCSEC
                     QGKRYQKEILSIQYKGKTIADILDMEVAEAKTFFSDCSDIYRKLSLLDEVGLGYLKLG
                     QNTLGLSGGESQRIKLAKELGTKTKKRMLYILDEPTTGLHFKDIENLLITFRKLVNEQ
                     HSLLIIEHNTEVIRASDWIIDIGPDSGINGGEIVASGTPDEIKINPNSITGHFI"
     gene            36090..38324
                     /locus_tag="ACGI59_000855"
     CDS             36090..38324
                     /locus_tag="ACGI59_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFG9738660.1"
                     /translation="MKLSRYKKNSLVLSIILCSLIISILVITVFNTVKLHNISNKQSK
                     NYLNDVSTQIVMNVDSKIKFILSDLRIMADFIKQYEGDSRYEYLSKRKTSYKYYDVGI
                     IDLEGKAEFLSGKKFDLKNTVSYKKGIEGKEYSEVIESMGFVLYSVPIFNDNHEVSSI
                     LIGVSNKESMNEILNIDNFNGKGTIEIINSKGKPLFIGKNTELIKDLSHKYKSSGDEP
                     WAQKMLEDFKNDKSGNITITSSKGVQCLLTYHPITRGLNDWHFLLIVPEDAVLGELNK
                     LNSFTITMTFIITCIIGIITLILYIIRRKYVNQIENIAYLDSITNGINSTKFSMLVKP
                     LISESPDSTYMMIAMNIKDFKLINDCFGSEKGNMTLKHLYNILYKNIDENEEFVCRHD
                     ADLFYLLIKNRPVLEALELLYKIEADTNYFNKNRENPYFLRLSVGIYTIENHNEDLIT
                     IQDHANTARKSFNKTHQSDFSFYSDIERKRLISEKEISNLMEKALENKEFFMCLQPKI
                     DIKSGKISGAESLVRWKNSEKGIIYPSDFIPLFEKSGFICKLDLYILEETCKLISKWI
                     KEGKEVLRISVNVSRQHLNDKLFLEKYKHICNKYNVPTCLIDLELTESIFLENPEAID
                     IIEDIHSNGFKCSIDDFGFGYSSLGILKDFKVDIIKLDRSFFVSKNNIDRGKVVIKSI
                     IELSKRLGMKVTAEGIEELEQVEFLKNVGCDYIQGYVFSKPLLISDFENFAYENNKIK
                     NIHY"
     gene            38580..40610
                     /locus_tag="ACGI59_000856"
     CDS             38580..40610
                     /locus_tag="ACGI59_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFG9738661.1"
                     /translation="MISGRMLLIMNFLKGKNKTSYKDISKQLSIEERKVRYDINNLNI
                     VLRSLNKSVIQKMNKGVLIIPDDFQVVCINNNEYVFSLTERVSIMKIISMFKIDNLNL
                     EKLSKEFQVSRSSIKNDLNVLSSELERNQITITYDKSFKISGEEDIIYKQRLNILKSY
                     SYLFGKEKSDLSVFEKYLVNVIEKIFKGIYLIDIYNWSMNLLLQMGWTLNDESFTWYV
                     SNIFLFTWYIHSDEKHPLESASLSEPCFENKFDQEFETIIQKKLTKEQIFLLKSFVFF
                     TNKYASLNEDMDLISTEIIVQDLIYNMSEILEISFKEDMILYKGLLNHIAPLIERIKQ
                     NIQIYEELLHVIPDKYQYVLEATKVSIQKNRVLSQIKNENETILLAIHFLGSIHRNER
                     NNYKNILLVCGFGYGVIAMLKDSLVNDYQINIVESIPSYKLQDYKEWESIDFIITTSK
                     IVIDLPKTIIEINPFLQEEDYLKFEEKGIKRKNVLTNYMSIKKRLDFLEEDAQKRVLS
                     IICQELGYSDERILFRQLNLSDLIGIDTIRIIDGDMKWEDAVKISSNILANCDFVSAQ
                     YAENIIDILKNVGFYAVKDNEFALLHGNDSSLVKVSSISLLICKEAVSFGDKKVKIIF
                     CLASRDKKEQIPAIINLTKMVYKTNFIAMLESAKTKEEAEVLIHKYEKEVTV"
     gene            40607..41059
                     /locus_tag="ACGI59_000857"
     CDS             40607..41059
                     /locus_tag="ACGI59_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFG9738662.1"
                     /translation="MNKLVNVKCSFKIHVNSKEEAIISLVDVVSKEGYLIDKNQFLKD
                     VLKREETLSTYIGHGIGLPHSQSVGVKNSCITIGKLDTPIEWTEEGEKVDLIFLISVT
                     KDNENNLHLKILSKLARLLMHESFRNQIRCSDEQTVYNLIKEKIEEED"
     gene            41061..42116
                     /locus_tag="ACGI59_000858"
     CDS             41061..42116
                     /locus_tag="ACGI59_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HFG9738663.1"
                     /translation="MRILKFLQKHLMTATSYMIPFVVAGGILFALSVTLSGQAAVPET
                     GWLAKLNQIGAAGLALFIPILGGYIAFSMADKPGLAPGMIGAYLAKEVNAGFIGGIIA
                     GFIAGFVVLQLKKIKLAPTMRTLGSIFIYPLVGTLITGGIIVFLIGEPIASFMTWMTN
                     WLNGMSGVSKIPLGGILGGMIASDMGGPINKVAATFAQTQVDTLPYLMGGVGVAICIP
                     PIGLGLATLLFPKKFSKEERDSGKASLLMGCVGITEGAIPFATSDPVRVIPCIMAGSI
                     VGNIMAFLLGCLNHAPWGGLIVLPVVDNRLGYIASVLTGAVVVAVLMKLVKKDVKEDE
                     EIEEDLDESIELIFEEL"
     gene            42183..42491
                     /locus_tag="ACGI59_000859"
     CDS             42183..42491
                     /locus_tag="ACGI59_000859"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFG9738664.1"
                     /translation="MKIVAVTSCPSGVAHTYMSAESLELSAKKFGIEIKVETQGSSGI
                     DNELSLKDIDEATCVILTNDVEIRNMERFKGKKVVRMSVSEIIKKSDALIKKIKDTFQ
                     "
     gene            42595..43860
                     /locus_tag="ACGI59_000860"
     CDS             42595..43860
                     /locus_tag="ACGI59_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II D-tagatose-bisphosphate aldolase,
                     non-catalytic subunit"
                     /protein_id="HFG9738665.1"
                     /translation="MKKLPIKTVVQRLLNLQEEGKSATLLGIGPMSPNLLQASFELAK
                     DDDFPLMFIASRNQVDADELGGGYVNGWNQETFTKDIKKVADKVGFDGLYYLCRDHGG
                     PWQRDKERNDHLPVDEAMELGKKSYLADIEAGFDLLMIDPTKDPFEIGKVIPLDVVLE
                     RTVELIEYCENERKRLNLPDIGYEVGTEETNGGLTSTETYETFITRLKVELDNRGLPM
                     PTFIVGQTGTLTRKTEQVGTFNFRNAYDLAQMAKKYGVGLKEHNGDYLDDVTLLEHIP
                     SQIIATNVAPQYGTEETRAYLKLAEVECKLEKEGLVEKTSNIRHVLLVNAIECGRWRK
                     WVVGEQKNLTTEEIFKDEVLSNEILDIAGHYTFNNDDVKKEIEVLYDNLSKNNIDGQR
                     FVVDHIKRPLRDYAECYNLKGVTTRILNK"
     gene            43948..44664
                     /locus_tag="ACGI59_000861"
     CDS             43948..44664
                     /locus_tag="ACGI59_000861"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFG9738666.1"
                     /translation="MINLIFDVDDTLYNQLTPFYTAYNKVFSSIKDISIEDLYMSSRK
                     YSDEVFHMTENGEMPIKEMHIYRIMKAFEELGNSITEKDAQSFQDEYIYQQSQITLIP
                     EVERILNFSKERNINLGIITNGPSNHQRMKLKQLNIENWVDKSNIFISSEVGFSKPDT
                     NIFRVAENVMNLDRENTYYVGDSYRNDVLGAKKAGWKSIWLNHRGHELEELFYKPDFV
                     ILEHKDLISLFIKICSYKNM"
     gene            complement(44859..45152)
                     /locus_tag="ACGI59_000862"
     CDS             complement(44859..45152)
                     /locus_tag="ACGI59_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein"
                     /protein_id="HFG9738667.1"
                     /translation="MRNMIENLIKNNTYIDDKVIMLSMLSSAKASANMYLNSALTSST
                     PELRAIYSASLTQMVEGHTALTELSINKGWVKPYDKPIQQLTCSYKESQNVID"
     gene            complement(45158..45373)
                     /locus_tag="ACGI59_000863"
     CDS             complement(45158..45373)
                     /locus_tag="ACGI59_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9738668.1"
                     /translation="MRILSETEKISLAAIIKMESDGLLMQRAINMLISDEDLKRQSES
                     SILATEGRIKAIQQFIVENEILISEEV"
BASE COUNT        17650 a         4951 c         8024 g        15161 t
ORIGIN      
        1 agtatatttt atttaaaaat ttattaatac aaaggagatt tactatgaaa aataaattag
       61 tttacatatg tattatgagt ctattgctat ttgtagtagg atgttctaat aatccaaata
      121 aggaaagtac cattaaaaaa gactctaaga ataaccaaag ccaagttaaa gaagaaaaaa
      181 gagaacctgt gaaaaaagat ataactataa gtttcgctgg tgatgtaact atggggaact
      241 ataaaggttc atcatattat ggcagttttg accatgaatt taaaagacaa ggagaagatt
      301 acaattattt tcttaaaaat gtaaaacctg tatttgaaaa agatgactta acagtggtta
      361 atttagaagg tccacttact acagcgtcaa atgcaaaaga aaaaaaattt gcatttaaag
      421 gaaagcctgg atatgtaaat attttaaaat ctggaagtgt agaggcagta agtattgcaa
      481 ataatcattc agaagattat tttgaagaag gaatgaaaga tacacagttt atattggacg
      541 agaataaaat aaattatttt ggattaggga aagatgctgt tgtagatgtc aaaggaatta
      601 aagtgggtct attagggtat aatggattat caactgaata taatgaaaaa aatttaaaac
      661 aaatggaaga tgatattaag tccttaaaaa agaaatcgga tgttgttgta gtgtactttc
      721 attgggggat agaattagat tattatccag ataaaaaaca aaaagatttt gctcattatg
      781 ctatagacaa gggagcagat ttagttatgg ggtctcaccc acatgtgatt caaggaatag
      841 aaaagtataa aaataagtat atagcttata gtctagggaa tttttgcttt ggaggaaata
      901 agaatccttc tgatacagat agttatatat atcaacaaac ctttactttt tcagataata
      961 aattaacttc aattaaagag ccaaatataa taccaacttc aataacatct agtaactcca
     1021 gaaataatta tcaaccaaaa atattggatg gaagtgagaa ggatagagtt ttaaataaac
     1081 ttgaaaaaat aagcaaagat ttaaataaat aaaatgtaaa aaaaaataaa tatatatatt
     1141 tattttaata atttatattt tgtttagggt atggtaaatc gtggtattaa ataagtagat
     1201 tttaatagtt aaattatagt caattataat aaagctattt aatctacttt tttaatataa
     1261 aaatataagg gggaatttgt aatggcttgt aacacttgta aaatgtgcga aagtgcagac
     1321 aagaaattag aaagttttgt agcttcaaag gatgttgaaa cagcatttca tagaacagaa
     1381 gatcagaaag ttaagtgtgg ttttggactt cagggggttt gttgtagact ctgttcaaat
     1441 ggaccatgta gagtaactcc taaatcacca agaggtatat gtggagcaga tgcagacact
     1501 atagtagcaa gaaacttttt aagagcagta gcatcaggag cagcttgtta tttacatgtg
     1561 gtagaaaata cagctaagaa tttaaaaaat gttggaataa ctaaaggtgt tgttaaagga
     1621 gaaaaaacat taaatcaatt agcagagatg tttggaatag aaagtgatga aaagtatgat
     1681 aaatgtataa aggttgcaga caaagttatc aacgatttgt ataaatcaag agatgataaa
     1741 atggaattag tagaaaaaat agcttatgca cctagagtga aaaagtggaa agagttaggt
     1801 ataatgcctg gtggtgcaaa gtcagaagtt tttgatgcaa tagtaaaatc atcaacaaat
     1861 ttaaatagtg acccagtaga catgctagtt aattgtttga acttaggtat atcaacagga
     1921 ttatatggtc ttacactaac taatctttta aatgatgtta tgttgggaga accagttata
     1981 agaatggctc ctgtaggatt taatgtaata gacccagact acataaatat aatgataaca
     2041 ggccatcaac attcaacatt tgcaaatttc caagatagat taaaagatga agatgttata
     2101 aaattagctc aatctgttgg tgcaaaaggc tttaagctag taggatgtac atgtgttggt
     2161 caagatttac aattaagagg agaacattat caagaagtat ttgctggaca tgcaggtaac
     2221 aattttacaa gtgaggctgt tttatcaact ggagcaatag atatagttct ttcagaattt
     2281 aactgtacta taccagggct tgaaccaata gcagataaat ataaagtaaa aatggtttgt
     2341 ttagatgatg ttgctaaaaa agcgaatgca gaatacatag gtttagacag aagtaaatta
     2401 gatgaactta gtaacacttt aatagaaaaa gcattggagt cttataagga gagaagagga
     2461 agtattgaaa tagatatacc aaaagaccat ggatttgaac aatctttaac tggtgtaagt
     2521 gaaaagaacc tgaaagcgtt tttaggagat tcatggaaac ctcttattaa tttaattgca
     2581 gaaggaaaaa ttaaaggtgt tgcagctgtt gtaggatgtt caaatatgac agcaggagga
     2641 catgatgtaa atactgttga acttactaaa gaattgatta aaaaagatat aatagtacta
     2701 tctgcaggat gttctactgg aggattagaa aatgttggtc ttatgtcacc tggagcagaa
     2761 gaattagcag gagaaaactt aaaagaagta tgtaagactt taggaatacc accagtatta
     2821 aactttggac catgtctagc tataggaaga ctagaaatag tagcaacaga attagcagct
     2881 gaacttggaa ttgacctacc acaattacca ttagtgcttt cagctccaca atggttggaa
     2941 gaacaagcat tagctgatgg agcgtttggt cttgcccttg gcttacctct tcatttagct
     3001 ttacctccat ttataacagg aggaaaattg gtgacagaag tattaacaga aaaattaaaa
     3061 gacttaacag gaggacatgt tatagtgaat ccagacccta aatcttcagc taatcaatta
     3121 gaagaaataa taatagatag aagaaaaaat ttagggttaa aggatgtgga atgcaatgca
     3181 tagaattttt ataaataaag atttatgtac aggatgtaag agctgtgtac tagcatgtat
     3241 gctaaaacac aataaagatt atgacatgta cactcttgat ttagaaaata tagataatga
     3301 tagtagaggg catatagaat tagatagtaa acataataat ccagttccaa ttctttgtag
     3361 acactgtgat gaacctgaat gtgtattagc atgtatgagt ggtgcaatgc ataaagatag
     3421 tgaaagtgga atcgtttcat atgatgaaga gaaatgtggt tcatgttata tgtgtgtaat
     3481 gtcttgtcca tatggattat taaagccaga tgatagaagc aagcagaaca tacttaaatg
     3541 tgatttatgt aaagatgagg agtatcctag atgtgtagct aattgcccaa gtggagcaat
     3601 tgaattacag aaggaggaaa atgatgaatt atgtagtgtt aggggctagt gcagctggta
     3661 taaatgcagt aaaaacatta agagaattag ataaagattc aaatatagta gtaatttcta
     3721 aagatgaaaa cgtatattca agatgtatgt tgcatcacgt tatatctgaa cacagaattt
     3781 taaaacaaat aaattttgtt gatgaagatt ttatggagca aaataatgta aaatggattg
     3841 caggtaagac tgttaaaggt atagacataa ataaaaaggt agttcagaca gaagacatta
     3901 ctgtaaatta tgataaactt ttaatagcta caggtgcatc ttctgctatt cctcctataa
     3961 aaaatttaag agatggaaat tttgtatatt ctgtgagaaa catagatgat atttataaaa
     4021 taaaagaaaa agcacaaaat tctaaaaatg tagttataat aggtgctggt ttagttggta
     4081 tagatgcatt agtgggatta tttaaatatg agcagttaaa tatttctgta gcttttatgg
     4141 aaaagtacat tttagataga caattggatg aatatacagc atcagtgtat gaaaataagt
     4201 tcaaagaaaa aggtgtaaaa ttttacccaa gtgcatcaat acaagaaatt gtattggata
     4261 acagtaaaaa tgttacaggt gttgcatttt caaatggtga agttttagat gcagacatgg
     4321 taattgttgc tacaggggtt aaaccaaatg ctgacttttt agatggaaca ggaatagaat
     4381 atgatagagg tataataata gatgatatgt gccaaacaac acaaaaagac atatatgcag
     4441 caggagatgt tgtaggtaaa aatgctattt ggcctctggc agtaaagcaa ggaattgtag
     4501 ctgcctataa tatggttgga aaagataaga agatagaaga tgaatttgct tttaaaaaca
     4561 gtatgaattt catggatata ccaactattt ctataggaat gaatacacct gtagatgata
     4621 gctataaagt gctaactaga catggaatta atgattataa aaagtttgtt tttaaagata
     4681 atgtaatcta tggagcagtt atacaaggag atatttcata tgtaggtgta ttaacttatt
     4741 taataaaaaa taaagttgaa atatatgatt tagaaaatag gatatttgat atatgttatg
     4801 cagacttttt taatataaaa gaaaatggag aattttgtta tagtgtatag tatatacgtg
     4861 gagtaaggtt tataaataca gtaaatcaat aaatagagtt attctaaaat aagaaaaatg
     4921 acaaatctta tttagaaata gctctatttt ttgtaattaa atttaaatta tattcaattt
     4981 gtaaaatatt attttgaaca attatacata tactctctag ttagaggtaa atcatttttt
     5041 atacctttag tgaaaagtat ttgatgtata tctataatac cagtgttgaa agcagcagca
     5101 caagaacaaa ggtataaatc ccacattctt ataaacttat catcaaacat ttctttaatc
     5161 tttggaagat tctcagagaa atttttttgc cagtatagta aggtttttac ataatgtaat
     5221 cttagacttt ctacgtctat agtatgatat ttgtaatcac tacaaatatg tatcatttct
     5281 cttaggctag gaataactcc tcctgggaaa atatattttt ttatccaagg gtctccataa
     5341 gattcctgca atccacttat atagtgaagt aaaaacaatc caccatcttt taaaacagca
     5401 tttacgtttt taatgaaaag ttcatagttt tctctaccaa catgttcaag catcccaacg
     5461 cttacaattc tatcaaacat aaggtctgat ttttttaatt ctctatagtc cataagtctt
     5521 acttcaagat agtcttgaag gttctcttct ttaattcttg cgttaaattc cttaaattgc
     5581 tcttcgctaa gtgtaattcc aacgccgtga attttatatt tttttgcagc ttctattagt
     5641 aaaaatcccc aaccacaacc aatatcgaga aggctcatac cttcctctaa atttagttta
     5701 gttaatatgt ggtgaacttt attgacttga gcatcataaa gagtatcatt atcatttcta
     5761 aagtatgcac aagaataact aagtgtctta tctaaccata agctataaaa atcgttgcca
     5821 atgtcatagt gggcatgtat ttctttcttt tgatgtctca ttgaatttga tgagtgcaat
     5881 agatgtttta cagctgaatt atcaatattg aatttgtcta tttgttttaa aagtagattt
     5941 aaagttaaga ataaatctcc ctcaacttca atgtctctat ttatgtaggc ttctccaagt
     6001 gctaaagatg tactttttaa tagttcagac ttgctaatat cattatttac tataactttg
     6061 aactgagggg agccctctcc aattagaaat tcttcctcat ctttaagcct aactagaaat
     6121 ggtgtttcac caaatttctc taaatagtct tttaaaaaat tttttgtaat attcatatta
     6181 ccagctcctt ctaatgtaaa tccagtaagt ttccctgctt aaaatatatt atagtccttt
     6241 ttttaaaaaa gtaaaatttt ttcaataaat tttttattta aaggaaattt attttttata
     6301 aagaaatttc atgttgtagt atattttttg cttataaatt ttggtactgg atataatatt
     6361 aaactgttaa tgatataata aatttaatct ataaagtaaa taaaattttg taataaatag
     6421 ttaaagctat tttataagga gatatatgat ggaagtatat gctttggttg ggtcaagtgg
     6481 cacagggaag agctttaagg ctttagagtt cgcatatgaa aatgatatag aatatataat
     6541 tgatgatggg attttaatat acaaaaacaa ggttttagct gggatatcgg caaaacaagc
     6601 aaatactact atagaagcag ttaaaagggc tattttttat aatctggagc atagacaaga
     6661 agttagagaa gcaattaaaa aagaaaatat aagaaaaatt cttattattg ggacatcaaa
     6721 aaaaatggtt aatcaaattg tagagagatt gtcaattgga agggtttgca agtttataaa
     6781 tatacaagat ataagtacta aatatgaaat agaaatagca aaacaagcta gaaaagaagg
     6841 aaatcatgta attcctgttc cagcagttga gataaaaagt atggcaagtg gtcttagtat
     6901 aaattctttg aagagacttt ttagaaaagg aagtaatagg aatatggtag ttttagaaaa
     6961 aacaattata agaccaactt ttagttatat aggaaagttt tatattagtg ctgatgtaat
     7021 taaacagatt attgaatatg aagtatacaa ctttggaagc attgatagaa taaataaaat
     7081 caatatagag aattgcaata attttatgaa tatttttgta agtataaata tcaatgattt
     7141 agaaactata aaaaaaattg aagacataca acagacaata aaaaagtcta ttgagaaaat
     7201 gactttagtt aatgtacaaa aaatagatat taacattcat aaaattaaaa aaataaactt
     7261 cctccaatgt taaaaaataa actgagaaaa tgatatacta atttgcaata aagtgtgcta
     7321 taattaaact gtaaatttag gaaatggttt tttctaaatg gagtatacat aattataacg
     7381 tatttataag ggggctttca aatgtcagga aaagatgtaa atgtcttcga gatggcgcaa
     7441 tctcaagtaa aaaatgcatg tgataaatta ggtatggaac cagcagttta tgaattatta
     7501 aaagaaccta tgagagttat agaggtttca attccagtaa aaatggatga tggttctata
     7561 aaaactttta aaggatttag atcacaacat aatgatgcag tagggccaac aaaaggtgga
     7621 ataagattcc atcaaaatgt ttcaagagac gaagtaaaag ctttatctat atggatgact
     7681 ttcaagtgtt ctgtaacagg tataccatat ggtggaggta aaggtggaat aatagtagat
     7741 ccatcaactt tatctcaagg tgaattagaa agattaagta gaggatacat agatggaata
     7801 tataaattaa taggtgagaa agttgacgtt cctgcaccag atgtaaatac taatggtcaa
     7861 ataatgtctt ggatggttga tgagtacaat aaattaactg gacaaagttc tataggtgtt
     7921 ataactggta agccagttga atttggtggt tctttaggaa gaacagctgc tactggattt
     7981 ggtgttgctg ttactgcaag agaagctgct gctaaattag gaatagatat gaaaaaagct
     8041 aaaatagctg ttcaaggtat aggaaacgtt ggttcttata cagttcttaa ctgtgaaaaa
     8101 cttggtggta ctgttgtagc tatggctgaa tggtgtaaat cagaaggttc ttatgctata
     8161 tacaatgaaa atggtttaga tggtcaagct atgttagatt atatgaaaga acatggtaac
     8221 ttattaaact tcccaggagc taagagaata tctttagaag agttctgggc ttcagatgtt
     8281 gatatagtaa taccagctgc attagaaaac tctataacta aagaagttgc tgaatctata
     8341 aaagctaaat tagtttgtga ggctgctaat ggaccaacta ctccagaggc tgatgaagta
     8401 tttgctgaaa gaggaatagt tcttactcca gatatattaa ctaatgctgg tggagttaca
     8461 gtttcttact tcgagtgggt acaaaactta tatggatact actggtcaga agaagaagta
     8521 gagcaaaaag aagaaatagc tatggttaaa gcatttgaat ctatttggaa aatcaaagaa
     8581 gagtacaatg ttacaatgag agaagctgct tacatgcatt caattaagaa agttgctgaa
     8641 gctatgaaat taagaggatg gtactaagat ttactcttaa taaataatag gatataatta
     8701 tttaagatat aaaggggctg tacatagtac agcccttaaa ttatgtggct tacaatgaag
     8761 taagctattt ttgttttaac ttataaaaaa cttgataaat tatgtaattt agaatgtatt
     8821 ttttaccacc acatgattac aaattagaag agttagatta gatttttaga catgaactta
     8881 tgcattttaa aagcaaagac cttaatttaa agtatctagt attattttta aaaatagtat
     8941 attggtttaa tccatttata tatataatgg aaaaatatat ggatttggat tgtgaacttt
     9001 attgtgatga gagagtttta aaaaattgta ctgtacaaca aaagcaagaa tatgctctaa
     9061 ctattgtaaa ttcaatgaaa agaagtacaa atttttcaaa taaatttgta gctggattaa
     9121 ataaacaatc tgatattaga aaaagagtaa taaacatgtt cagtgaaaaa cataaaaatg
     9181 gtatcttaag ggctttgatt ataagcttgt tttcttctat tacatttttt aaattcgatt
     9241 caactagtat tattgaagag aatgatttct tagagaggtc aagatatacg tttgaaggaa
     9301 ggcaagaaga catgtttgta acaatagatt ttatatatgc agatgtacct ataaaactta
     9361 gaaaagagca tgaagaaaac tgtagagtca atggaatagt accaaaagat tcagattcaa
     9421 tagaaattat tcctagattt tataaagatt gtatggattt aaaaaaataa gttagactta
     9481 atattgatgt tgatattatg aattttttat atttaagcaa ttgaaaattt tattatagat
     9541 tcaattttat atgtgtttaa gtatgaaatt tatagatagt tgaaataatt aaatatgatt
     9601 gatgcatagg aatcattact aagctataat taattataag ggagtttaaa taaattatga
     9661 gttacacaat tataaaatga taaaaaatag ttatctgtta caaaaaatgt atctttttaa
     9721 ttaataaaga taatatgaaa acttaaaagg caaataaact taaacataaa atactcttaa
     9781 ataagatagg agaaaaatgt atgtctaact ttattctaaa tgggattttt agaacagtta
     9841 tagtaagtag tctaagcatt cttatgatat tgattttcaa gaaaaatgta tttaaaagat
     9901 ttagcaaaaa atttaattat tatatatgga tgatagttgt tattaaatta tttttaccat
     9961 ttacatatta tacttttaca ataaatatct tgagaagtaa aaaacacatt aatattaata
    10021 acataaattt agagggattt aataatgttt caactataag taataatatt atattgtaca
    10081 cttggattat aactgtagta gtttatttaa tttacactat atttaaatat ataaaactta
    10141 aaaatttaat aaatgactta tcttatgatg ttgatgatgg agagattgta aatctttata
    10201 aaaatatatt agaagaattt aatataacaa aagatataaa actcaaatac tcttatgaag
    10261 ttgaaacacc agcatttttt aattcatgtg tgcttttgcc accacatgag tataaattga
    10321 aagagttaga ctggattttt agacatgagc ttatgcattt taagagtagg gacctttatt
    10381 taaaatatgt aatcctgttt ttaaaaacag tatattggtt taatccattt atatatataa
    10441 tggataaaca tatagattta gattgtgaac tttattgtga tgaaagagtt ttaaaaaatc
    10501 gaaattcaga ggaaaaaaag gattatgctt taacaattct aaatgcaatg agaaaaggtt
    10561 caaatacttc aaataaattt atagctggac tccataagca atctgatatt aaaaagagag
    10621 ttatgaatat gtttaatgaa aagtacaaga atggtatatt aatggcatta actttatgtt
    10681 tattatcttc tattactttt ttgaaggtta attcaacgag tattgttaat cttaatatat
    10741 tgccaaaagg aatggaagaa aaagatgtta ctaatagtgc aattgttaca atgatagaat
    10801 ttacatatgc agaagctcct aaaagtatga gagaagaaca taaaataaat tgtgaattct
    10861 taggtttaat acctaaagac tctgataaaa taggtgttgg tatagaatat tatgacagtc
    10921 tattgaatga ttaaataaat attattttaa cagatattaa ttataaagtt ttgaatagta
    10981 atttgttgag aaataaatat atccttataa aaatagatac ttatatacca agaataaaaa
    11041 atagtcaaga tagtacacaa ggatatactt ataaagaact caaataagtg aaataaatta
    11101 ttaattataa ggtttataat tttaaaaaat tagataaatt caggtgtaaa atactataaa
    11161 acacaattcg gaaaagtgta tgttgaactt catttaaaat ggaatttcaa agttgctata
    11221 gtaagtagtt taagcattct tcttatatta atctttgaga ataatgtatt taaaaaagtt
    11281 agtaaaaaat ttaactattg tatattaatg atagttgcta ttgatatatt atctaaaaaa
    11341 attaagtatg aaaagattac tgctaggaca atcttttcat acttaatttt taatatttat
    11401 attatccaaa attttcatct ttttaactta gaatttgaga atcactatta ggaaataggc
    11461 tttgatttat gtagggttaa aaatttcaca gaatatctaa ctttatacta aatccatcaa
    11521 aaaatatacg ccatataaaa gcgcataaaa aaacacataa aacaatattt atgcgcactt
    11581 taacctcgaa tggtacaaaa ttctttactt ttacagaccc atattatata attaagtaaa
    11641 taatgtatat ttaggtgaca tattggataa gctaatttat ttattttatt aatataatga
    11701 tactataaaa gttatataaa gtggtttaca aaaaagttac agatagagct tttattaatg
    11761 tattgtgtat tagtatttta tccttaggta cacttcacct acaaaataga atcataagat
    11821 aattatggga aaattttagg aggtttatcg tgaagaaaaa gatattaata ccagtatttg
    11881 catcagttat ggcattatca gtaagttcaa ttgtaaatgc agatgaagta aatgattcat
    11941 ctcaaaataa ggatgataaa acaaatactg aattaaattt aggtgaatat aaagaagtaa
    12001 aatataaagt tgcaaaaata aaagatggag tagctataaa gataagagaa gaaggtcaag
    12061 ttcaaaacat agcttattct ggagatgaat ttacggtttt aggaactcaa ggagaatggg
    12121 ttaaggttaa agttgaagat ggcgaaggtt ggttagctac tagatatgta gatatatctg
    12181 aaggtgtagg atatactaac gctgataaag taaacttaag aaaagataag agtgaaagtt
    12241 cagaagtaat tgaagagctg gaaaaaggaa gttctttatt ggttttggaa gataatggag
    12301 attggttaaa agttaaagat ggggaaacag aaggatatgt aaaatcatct tatatatctg
    12361 ataaagctcc tgtaatagaa gagccacaag taaacccaag tattgaccaa aatgtagatt
    12421 ctacaaataa taatcaacaa aacaatgcaa atactaatca acaaaataat aattcaaatg
    12481 tgccaacagc taactctaat gcagttcaag ctgtacttaa cttggcatat tctaagcaag
    12541 gttgtccata tgtttgggga gcagaaggac caaacacttt tgactgttct ggatttacac
    12601 aatatgtgta tagaaatgca gtaggaaaga atataccaag aacatctaaa gcacaatcta
    12661 aatatggaca aactgtaagt aaagcaaatt tacaaccagg agacttagta ttcttcacaa
    12721 ctaatggttc tggttctgtt agtcatgttg gaatatatgt aggtgggggt aatatgatac
    12781 attcaccatc aacaggaaaa acagttagtg taactagtat aaattcatca tactatacag
    12841 caagatttgt tacagcaaaa agaatattat aatcggtttg taatatgaca aattgataaa
    12901 tacataaata tatataaacg ctctatatta tatttaattt aatcatggaa acaaaaaatg
    12961 attatatttt aaatgtacta tagagcgttt ttgtatcact ttaaatctaa tcaagattca
    13021 agttaatatg actgttaaat aggagccttt tcatatgatg attatttgcg caatcccttg
    13081 acaggatagg gcaaaggtgg taagctacta acagtacaaa aatactttta attttagttc
    13141 agagaggcta aaaaaggagg acgaaagatg ttaaaatcaa gaaatttaat ccagccagaa
    13201 gacttttcaa tagaagaaat agatgaaata ttagaattag ctcaaaaaat aattgacaat
    13261 ccgtcaaaat attctcgtat atgtgaagga aaactattag ctacattatt ttatgaacca
    13321 tcaacaagaa caagattaag ctttgaatct gctatgaaca gattaggagg acgtgtagta
    13381 ggcttttcag agccaaattc atcctctgca tcaaaaggtg aaactctagg agatacaatg
    13441 aggatagtat ctggttatgt tgatataata gctatgagac atcctcaatc aggtgcagct
    13501 tcagaagcag ctagatatac ggaagtgcca tttataaatg caggagacgg caaaaaccag
    13561 catccaactc aaacacttac agacttactt actataaaat cattaaaagg aactttagaa
    13621 agccatacaa taggattatg tggtgattta aaatatggaa gaactgtgca ttctttggtt
    13681 aaggctatgg caagatataa aaatacaaag tttgtattta tagcgcctga agagttaaaa
    13741 atgccagatt atataaaaga agctataaag ggacatgcat attatgaaac taataatttg
    13801 gatgatgtta taggaagctt ggatgtttta tatatgacta gagttcaaca agaaagattt
    13861 gaagataaat cagagtatga aagacttaaa aactattata ttttaaataa agctaaatta
    13921 gaaaaggcat ctaaagatat gttagtaatg catccattac caagagtaaa tgaaatagac
    13981 atagatgtag atagtgatga tagagctgta tattttaaac aagctaaata tggaatgtat
    14041 gttagaatgg ctttaataat aaaactttta ggtataaatg aagactaata gttatcatat
    14101 aatagtaaaa caaaggagat tttgggatgt ataaaattct ggaaaatata tatataggcg
    14161 aagatatgta tagaatgaaa gtaaaaggta attttgaagg taaaatggga caattctata
    14221 tgttaagagc atgggatact tatccagtac tttcaagacc tataagtata catgatatag
    14281 atgaagaagg gataactttt ctttataaag tagtaggaga aggaactcaa attttgagta
    14341 acctaaaggt aaatgataat ataaaattag aaggccctta tggtaatgga tatgctaagg
    14401 ttgatggaaa ggtagctcta gtaggaggag gaatcggtgt agctccacta tatttggttg
    14461 ctaaaaatat aaaaaattgt gatgcatatc ttgggtttag agaagatgtt atattagaag
    14521 atgaatataa acaagtatgt aataaagtct atacaacagt aggaaatact tttgtaacag
    14581 atataattga tgttgagaaa tatgattata tattaacatg tggtccaact ccaatgatgg
    14641 aaaaattagt taagatggta gaaggtacta aaacgagaat tatggtatct cttgaaaacc
    14701 atatggcttg tggagtaggt gcctgtttag tatgtacgtg taaaactaat ggaggaaata
    14761 aaaagacttg taaagatggt ccagtgttct ggggagagga cgtgattttt aatgggtaat
    14821 ttaagtgtga aatttggaag tgtagaattt aaaaatccag taataatggc gtctggaact
    14881 tttggatttg gaaaagaata taatgagatt tatgatatac aaaaacttgg tggtataagt
    14941 agcaagggtc ttactttaaa taaaaaacct ggtaacaatg gtatgagagt tcatgagaca
    15001 tcatccggta tgatgaatag tgttgggctt gaaaaccctg gagttcaagg atttatagac
    15061 tatgaattac ctttttttag taaactagac ttagtcagaa tagctaatgt tggaggtggg
    15121 acattagaag actacttatt aggggttcaa atgttaaatg acaagcctat agacattatt
    15181 gaattaaata tatcttgccc aaatgtcaaa gctggtggaa tggcttttgg tataaaaaat
    15241 gaagtggcaa gagaagttgt tcgtgaggta agaaacataa cgaaactgcc tttagtaata
    15301 aaattatcac ccaatgcaga agatattgta ggaatggcaa aagtttgtga agaagaaggt
    15361 gctgatggag tttcattagt taatactttt aaagcaatgg caattgatat aaaaaataga
    15421 agaccagtat ttgaaaatgt atatgctggg ttatctggac cagctataaa acctatagct
    15481 ttgagaatgg tacatgaagt gtgtaaaaat gtaaatatac ctgtaatggg aatgggcgga
    15541 ataactaaag ccacagatgc tatagagttt ataatggcag gagctacttg tatccaagtt
    15601 ggaactgcaa attttatgaa tcctagaata ggtattgaaa taatagatgg aattaatgaa
    15661 tttatggata gagaaggaat aaaaagttta gacgaaataa gaggaattat ataaggtaga
    15721 ataaatagta ttttaaagat aaaaaatata gattaaataa acaataattt tataacatta
    15781 acatggagga aaaataatat gagtaaagta gatgtagtgg atatattaaa gaaaagtgat
    15841 gcattgttgg agggacattt tttattatct tcaggaaaac acagtaatag atacgtacaa
    15901 tgtgcaaaag tattaagatt tccagaatat gctgctgagg tattaagtac agttgttgaa
    15961 caaataaaag acttagatat agacttagta gtaggaccag ctatgggtgg agtaatagtt
    16021 tcttatgagt taggaagaca attaggaaaa gaagctgtat ttactgagag aaaagacaat
    16081 acaatggagt taagaagagg atttgaagtt aaaaaaggag caaagataat aattgctgaa
    16141 gacgttgtaa ctactggtaa atcaactatg gagacaaaaa gagtattaga agccttaggt
    16201 ggagaagttg taggtgttgc atgtatagca gatagaacta atcatgatat aggtatgcct
    16261 atatatagtg ctataaaact tgatattcaa gtttatgaat ctgatgagtg tcctttatgt
    16321 aaggaaggaa aattaccagt tgttaagcct ggaagtagag agttcaagga attagggatg
    16381 taataaaaac ttaattattt atagtgttac ttaaaaaatg taaatttttt agagtaaaaa
    16441 tttgtaatat aacatataac ttaaaaataa aaagcagtta gccttataaa tgaaggttag
    16501 ctgttttttt gttaaaaata tatttacaaa ataagagata gtagcagtac aatataagta
    16561 taaggtaaaa gtattatata ttagggaggg gtaagtatga aaaatcaatt aaatgaaaat
    16621 cataaaaagg ttgtattttt tggagtaggt gctgtaggag ctacttttgc agaacaattt
    16681 tttaactcta aatatgattt taaaattctt tgtgataatg aaagaaagaa aagatattta
    16741 gaagaaggat ttataataaa tggaaaaagg tatgattttg attatgtaac taaagatgag
    16801 tataaacaag aggctgattt tataattata ggtctaaaat ataataattt aaaagaaaat
    16861 ataaaagaat tagatggatt agttggaaaa aatacagtta taatgtctct gctaaatgga
    16921 gttgatagcg aagagataat aggagaaaga tttggaattg aaaaaatggt atattcatat
    16981 gttaccaata tagatgcaaa gaaaatcaat aataatatta tacacactac taatgggata
    17041 attgtatttg gtaacaaaga taatagtgaa gatagaaaaa ctaatataat aaccgaagtc
    17101 tttgatgatg taaatataga atatacttta tcaaaagata ttcaacgaga tatgtggtgg
    17161 aagtacatgg ttaatattgg tgtaaatcaa acttcagcta tacttggtgc accttatgga
    17221 gtttttcaga gttctgagca tttaagagaa ttagcaaaat ctgcaatgag ggaagttgtt
    17281 gctatagcac aagcaaaaga catatctctt acagaagatg atgtggaaca ttcattacat
    17341 agaatactag aacattcaaa agaaggaaga acatcaatgc ttcaagatgt ggaagctcat
    17401 agacttacag aagtagatat gttttctaag aatatctgca aacttggaaa aaaatataac
    17461 atacctactc ctataaatca gactttcttt tatatgataa aagtaattga aagtagattt
    17521 taaagggtct ttgtaagaaa tattgataat tattgatttt aaattttatt aatgttatat
    17581 actatttaag ttactaaata tttttaactc ttcaatgaaa atagttgctg cttttgataa
    17641 agttatatct tttggaaata tatatgagaa atgtctgttg tagctgcttc caagctctat
    17701 gacagacagc tcattgttta gaacaggtaa gcttgtcact agattagaga ctattgtaat
    17761 tcctaaatta tttctaacag attctttgac agcataatta cttccaaaaa ccatcatact
    17821 ttttggaata atttctttaa cacttaaaaa catatctaaa taatctctgg ttccagaacc
    17881 atcctccctg actatccatt tttggttttg aagtttatca aaactaaaat cttttaataa
    17941 atgactcttg tatggaagtg ctaaaaccat cttatcttca aaaaaatatt cttgaataaa
    18001 tgaaggggaa gagcaagtgc cttcaataag accaatgtct aaaatataat ccttaacatg
    18061 agaagatacg attgatgtat tttttataaa aacttctaca tctatgtcag gatacttttt
    18121 agaaaataat gctagaaaat ttggtaaaat atactctcca atagttaaac ttgcaccaat
    18181 ctttaagtga ccagttatag catctgaaac ttggcttaca tcatgataag taatatttaa
    18241 aagatttaat atttctttag ctcttttgta caagatttgt ccactttctg ttataaaaat
    18301 ggttttttgt ttaatagacc tgtttataag cacaacttta aaataatttt ctagattttt
    18361 tatgtgttta cttacacttg gttgtgatag atttaaatat tccccagctt tagtaaagtt
    18421 tttgtattca acaacagcta caaatgtttt taattcttca aacaattttt tgtctccttt
    18481 acctaatgaa attttacatt ttttatatac tttctacaga taattatact atatataata
    18541 accattaaaa acattaatca ttacgatatt atatatttat tttacttatg gttattacaa
    18601 ttatatacta ttgctatgaa gaaaactata ttaattataa ggtaggtata ttatgttaaa
    18661 gaaaaaaagc agtttgacat taaaaactat aagggaaata ttaccaggac tgtttgtatc
    18721 agtattagta ggttatatta gtatatttat ttctatttta attcctaagg taggggcagc
    18781 atcaatttct atatttttag gtatgtttgt aggaaaccta tttttaaatc aaaaggtatt
    18841 tcaaaaaggt tataaatttt cagagacaga tttgttatca tattcaatag tactattagg
    18901 agcaacactc agtgtatcta ccttaataga cttaaaagtt tcaggaattt tatttataat
    18961 attgcaaatg acaataacaa taatagctgc actttatata ggtaaaaaac taggatttga
    19021 agaaaacttt agatttctaa tggcaagtgg aaatgcagta tgtggttctt ctgcaatagg
    19081 agcaacagtt ccagtggtta atgcgacaga taaagaaaag ggaattgcag taactattgt
    19141 aaatgttact ggtatatttc ttatgttttt acttccaata atatctcaat ttttatatag
    19201 ccatgaactt gtaaaaactt cagcaatgat aggtggaact ttgcaatcaa ttggtcaggt
    19261 tgtagcaagt ggagcaattg ttggagaaaa tgtaaaagat ttggctacta tttttaagat
    19321 agtaagagta atatttctag ttgttgttgt attagtattt ggacatatga aaaatcaatc
    19381 taatagggaa attcttgaag aagaaaagga tgagataaaa aagaaaaaag tgactatacc
    19441 atggtatgta attggattct ttataacttg tgcacttttt tcaatgaaca ttataccaaa
    19501 agaagtatct gtgttgtgca aagaaataag taacaaacta gaaataatag ctttagcagc
    19561 aattggatta aaggttaatg taaaagattt agtgaaacaa gggaaagaag tttctttata
    19621 tggtttattt gtaggtatag tacaagttgt atcagctgta gttttaataa aaatatttat
    19681 atagcataaa aatagtattt gtaatattaa tttgattaac taaaataata tgatactaat
    19741 aatttataat ataaataaaa aatgaatttc gataagttta agtgggtata aacttataga
    19801 aattcatttt ttatttaagg taaaaattta aagtaaaata ttattttaca taataataac
    19861 cttataaatt ttgaaattac ttatctttgt gttaaaataa gtaaaatgca catattagag
    19921 ctaagttttt aagtaaataa acaaaatgaa aatataaata ttaaggagat ttggtatatg
    19981 aatgtttatg aaaaaggtaa tggagttata ctagaaggag taacagattt tgacccagta
    20041 catatatttg agtgtggtca atgttttaga tggcacaaac aagaagatgg ctcatacact
    20101 ggagttgcaa agggaagaat attaaatgtc aaaaaagaga atgataaaat atatttaaat
    20161 aatactaatt taaaggagtt taacagcatt tggtataatt actttgattt aggaactgat
    20221 tatacagaaa taaagaataa gctaaaaaat atggatgaat atttaaataa ggctactgaa
    20281 tttggatggg gaataagaat attaagacaa gatggatggg aaatgcttat atcatttata
    20341 atttcctcta ataataggat tcctatgata caaagagcga tagaaaattt atcaagaaag
    20401 tttggtaagt atataggtga atatgaaggt aatgaatact atgcattccc aacaccagaa
    20461 gaactaaata aggcctcaca agaggaaata agagcatgtc aaacaggttt tagagataag
    20521 tacataaaaa gtactacaca ggcagttata gagaataatg atgaggtatc tgaatataca
    20581 aatttaagta cagaggattg tagaaaagag cttttaaaat ttaatggagt aggaccaaaa
    20641 gtatgtgatt gtattgcatt atttggtatg caaaaatatg attcttttcc agttgatgtt
    20701 tgggttaaga gagttatgca agaattttat atagatgaag acatgagttt acctaaaatg
    20761 agaacttatg gaatcgataa atttaaagaa atgtctggat ttgctcaaca gtacttattt
    20821 tactatgcaa gagaactcgg tataggaaaa taaattaagg gggagaaatt atgggagtaa
    20881 taggaacaat atttctgttc tatttaatta tatcatatct agctggagcc ataatttctg
    20941 tgattatatt gcttgaaaat agagaccctg caaaaacaat gtcttggcta ttgatgttta
    21001 ttatatttcc aggtgtaggt ttaatgatat atgctatttc aggtagaaat attagaaaaa
    21061 gaaaattatt taagactcaa aaactagcta ataatataaa agaaaagaaa ctatttgata
    21121 cattagaaaa gataacagaa atagttgaat tagaaaaaga atctataaaa caaaataagc
    21181 ttttaagaga tgaagaggat ggtagttata gaaaaagggt aataaatatg ttgttaaaga
    21241 ctggtatgtt tccctttact aaaaataata aagtggatgt atttgtagat ggaaatgaaa
    21301 agtttaaaag acttatagaa gatataagag aagctaaaga ccatattcat ttagagtact
    21361 ttatcatcaa agatagtgaa ataggtaggg tgttaaaaga agaacttata aaaaaggcta
    21421 aagaaggcat taagataaga attttatatg atgatgtagg atgctggaga ttttggttta
    21481 atagaaaatt ttttcgagaa atgagggaag taggtataga aattgcagca tttttaccta
    21541 caaaatttcc tataatagga gggaaattaa actatagaaa tcacagaaaa attgttgtta
    21601 ttgatggaat aattggatat acaggtggaa taaatatagg agatgaatat ttaggcaaaa
    21661 atgataagtt tggatattgg agagatactc atattagaat taagggtatt tctgtataca
    21721 tgcttcaaat gacatttctg attgattggt attatacgac aaaagaagta ttagtgacta
    21781 aaaattattt cccaagtgta gggaatgttg gtgaaagtat gatacaagtt gttgcgagtg
    21841 gtccagacag tgactgggaa gatattcatt atgcttattt ttctgcaata tgtcaggcta
    21901 gaaaaaatgt ttatatagaa actccatatt ttattccaga tgaaagtcta ctaaaagcta
    21961 taaagagtgc tgctcttagt ggagttgatg tgagaataat atttcctaaa attgcagacc
    22021 acaagatagt aaatatagca tcttattcat attttgaaga aatattaaga gcaggtggta
    22081 aagtttattt atataataaa ggatttatac attctaaagt cgtaataata gatgataaaa
    22141 ttgcttctgc aggtacagct aatatggatt taagaagttt tatgcttaac tttgaggtaa
    22201 atgcatttat ctatgatgaa gaagttatta gggtaatgac agatgatttc tttgaggatt
    22261 taagtcactg tgaagaactt aatttggagg tatttaagaa tagaaatatc atacaaaaaa
    22321 tcaaagaatc tgtagcaaga ttattttcac caatattata aaaaattatt aaaaaaggta
    22381 ttgaaaatta tgtgtgattg gtatattata ataattgtta gcactctatt agatagagtg
    22441 ataataaata aaaagtatat atataaatct acatatataa gtagtattaa tttaaaaata
    22501 actaaaaaca taggcgaact agtctatact taatatttta ggaggcgtga ataatgaaaa
    22561 taagaccatt agctgacaga gtagtaatta aaaaagtaga agcagaagaa aaaactgcaa
    22621 gtggaatagt tttaccagga gcagctaaag agcaacctca aatagctgaa gttgtagaag
    22681 ttggaccagg tggaatagtt gaagggaaag aaataaaaat ggaattgaca gtaggagata
    22741 aggttatatt ccaaaaatat tctggaacag aagttaagat agaaggacaa gaatacacaa
    22801 tactaagaca gagtgatgta ttagctgtta ttgaataaat atagaataaa tttattagga
    22861 ggggtttaaa atggctaaag aaattaaatt ttcagaggaa acaagaagag ctttagaagc
    22921 tggtgtaaat aaattagcag atacagtaaa agtaacatta ggacctaaag gaagaaatgt
    22981 tatattagat aaaaaatttg gttctccact tataactaat gatggagtaa ctatagcaaa
    23041 agagatagaa ctagaagata gatttgaaaa tatgggtgct caattagtta aagaagtagc
    23101 aactaaaact aatgatgtag ctggagatgg tactacaact gctacagttt tagcacaagc
    23161 tataataaga gaaggtttaa agaatgtaac agcaggggct aacccaatac ttttaagaaa
    23221 aggaatacaa aaagcagtaa cagtagcagt agaagaatta aaaaatcaat caagaatagt
    23281 agaaacacaa gaggctatat ctcaagttgc ttctatatct gctggtgatg aagaagttgg
    23341 aaaattaata gcagaagcta tggaaatagt aggtaaagat ggagttataa ctgttgaaga
    23401 gtctcaaact atgaatactg aattagatgc tgttgaaggt atgcagtttg atagaggatt
    23461 tgtttctgca tatatggtta cagatgtaga taaaatggaa gcagttttaa acgacccata
    23521 tatattaatt actgataaaa aaatatctaa catacaagaa ttattaccag ttcttgaaca
    23581 aatagttcaa caaggtaaaa agttattgat aatagctgaa gatgtagaag gtgaagcatt
    23641 atctacatta gtagtaaata aattaagagg aacatttgat gtagtagcag ttaaagctcc
    23701 aggctttgga gatagaagaa aagaaatgct tcaagatata gcaatactta caggtgctca
    23761 agtaatatca gaagaattag gttatgattt aaaagaagct gatttatcta tgctaggtag
    23821 agcttcatct gttaaggtaa ctaaagaaag tactacaata gtagatggtt ctggagataa
    23881 aaaagctata gaagatagag taactcaaat taaacatcaa gtagagcaaa ctacttcaga
    23941 ttttgataga gagaaattaa tggaaagatt agctaaactt gctggaggag tagctgttgt
    24001 aaaagttgga gctgctacag aagtagagtt aaaagaaaga aagttaagaa tagaagatgc
    24061 tcttaatgca actagagcag ctgtagaaga aggaatagta gctggtggag gaactgcttt
    24121 tgttagtgtt ataccagcaa taggaacact aattgagagt ttagaaggag aagttaaact
    24181 aggtgctcaa atagttaaaa aagcgttaga agagccatta agacaaatag ctataaatgc
    24241 tggtcttgaa ggtgctgtaa ttgtgcaaaa tgttgttaat tctgaagcag aaactggatt
    24301 tgacgcattg aatgaaaaat atgtaaatat gatagaagct ggtatagttg acccaactaa
    24361 agttagtaga agtgctttac aaaatgctgc atcaatagca agtactttct taactactga
    24421 atctgctgtt gctgaccttc ctgaaaaaga agatgcagga atgccaggaa tgggtggcgg
    24481 aatgcctggt atgatgtaat ttaaaaaatt taaaatataa attttaatat aaagaactat
    24541 ttcgacttag attttaaact ctaagttgaa atagttcttt tgtatatatc taatttttgt
    24601 atatctataa attcaataca catatttaga taatcagtat ctagtttgta ataatatttt
    24661 agagtagtat tgttattttt taggttatga ttttagatat atattaatta aggtataatt
    24721 gttgatagag aataaatcaa atttaaaagg atgcgataat atggacttaa gaaaattgct
    24781 tgaagaagtt aaaaatgaaa atattgatat agattttgcg ttagaaaagt taaaagattt
    24841 accttatgaa gaccttggat atgcaaatat agaccatcat agagaattaa gaaatgggta
    24901 tccggaagtt atttattgtg aaggtaagag tgatgagcat attttaggga ttattgataa
    24961 aatgaatcaa aaaagttcta acatattggg gacaaggtgc agaaaagaaa cttttctgaa
    25021 aattaaagaa atctataatc atgctgagta cgaagaggcg tctaaaattc ttaagattca
    25081 aaatcatgat atagagaata taggaaaagg taaaattgtg attgctacag ggggaacttc
    25141 tgatatacca gtagcagacg aagcttatca tacagctaag tttttaggta atgatgttga
    25201 tagaatatat gatgttggag tggcaggtat acataggctt ttaaataaaa gacataaaat
    25261 agatagtgct agagtaatag tagccgttgc aggtatggaa ggtgctcttg caagtgtaat
    25321 tggtggactg gttgatgttc cagtaatagc tgttccaact tctgttggat atggagcaaa
    25381 ttttggagga ttagcagcat tgctagctat gttaaatagc tgtgcatctg gaatatctgt
    25441 tgtaaatatt gataatggat ttggagcagg atatttagca tcaatgataa ataaactgta
    25501 aatacattga tataaattaa acaaaactat ataataaaat ttaatttcta ttatattttt
    25561 actattaaaa ttgaggagac atatatgagt gaaaaaatac tttattttga tataataagc
    25621 ggtatatcag gggatatgac ccttgcaagt ttattaaatt taggtgtgcc taaggaaatt
    25681 tttttggagg aaattcataa actaagaatg agcgatgaat ttaatataaa catcagctcg
    25741 aaaactgaaa atggaatagt aggtacaaaa gtagaagtaa ttacaaaaga aaaacataca
    25801 catagaaatt tagtggatat atttgaggtt atagataaaa gcactctaaa cgaaaatgta
    25861 aaatctaaag caaaaaaaat atttatggta attggagaag cagaagcaaa agttcatgga
    25921 actacaatag acaagataca ttttcatgag gtaggggcta tagattcaat tgtagatata
    25981 gtaggagctt ctatattagt tgacttactt tgtgtagata aagtttgtgc tagtacagtg
    26041 tctgttggtt caggatttgt aaaatgtgaa catggaatta tacctatacc tgcacctgca
    26101 actatagaaa ttttaaaagg tgtgccaatt aaattaaata atgtaaatgg agaatgtaca
    26161 actccaacag gagctgcaat cataaaagtt ttgtgtgatg agtttgtaga tgagcttgaa
    26221 ttttctccaa agcaaattgg atatggtata ggtcataaga agtttgaagt accaaatata
    26281 ctcagaactg tattaggtga aaaaaaaaag aagaattaat ttatgaaatt agtgcaaaca
    26341 ttgatgatat gtcttcagaa atctattctt atttgtatga aaaaatatta gatgagaaag
    26401 cattggatat ttatacagag agcatttata tgaagaagaa tagaccagca acaaaagtat
    26461 caattttatg tagtgaatca gatttagaaa aatttattaa aatattactt ttagaaacta
    26521 gtacttttgg agtgagatat aatgcatata atagagaaat tctagagaga aaatttgtaa
    26581 aattagatac aaaatatgga gtggttacag taaaactagg gtattataaa ggaaaactag
    26641 tcaaggctac tccagaatat aatgaatgta aaactatagc aaaaaatgaa gatattccta
    26701 taaaaaaagt gtttgctgat ataaatttta gaataaaaga agaatttagt gaaaacttat
    26761 tgacataagt aataaatttt gataaattaa tattcagcct tattaaataa aagtaaacag
    26821 aaaaaaatat ataaaatata aactcactcg tatatgctca gaaatatggt ctgagagtct
    26881 ctaccaagat accgtaaata tcttgactat gagtgaaatt attataccaa aagacctagt
    26941 taagtaaatg tgtaggctga gttaaactcc ctatgtgttg gctataagat aaaggtattt
    27001 tggaagcctg tatagtaatt tcacttttgc tagaaattat ggaagttact atacaggttt
    27061 tttgtgtaaa aagatttaat ataatttaga ctgtattgtg ttaaaataaa tatataaaaa
    27121 ctatattata caaggagaga tgtgaattta tgaaacatga attagtactt gtaatagatt
    27181 ttggaggaca atataatcag ttgatagcta gaagagttag agaaaacaac gtatattgtg
    27241 aaatacttcc atgtactgca agcatagaaa gaataaaaga aaaaaatcct aaagggataa
    27301 tatttacagg cggtccaaat agtgcgtatt tagaagattc tccaacaata tcaaaagaaa
    27361 tatttgaatt aggagtacca atacttggaa tatgctatgg tattcaaata atgtctcatg
    27421 tattgggtgg agttgtaaga aaaggtaata aacaagaaaa agaatatgga aaaacagcta
    27481 taacttatgg aaaatcatca ttatttgaag gaataactac aaatagtgta tggatgagtc
    27541 atacagattt aatagaaaaa gttccagaag gatttactat agttgcaaat actaatgatt
    27601 gtccagtagc agctatggaa aatgtagaac gaaacctata tggagttcaa ttccatccag
    27661 aggtggaaca ctgtttggaa ggggataaga tacttacaaa cttcttatat aatatttgta
    27721 aagtaaaagg tgactggact acagattcat tcatagaaga taaaataaaa gaattaaaag
    27781 aaaaaatagg cgataaaaaa gctttatgtg ctttaagtgg aggagttgat tcttctgttg
    27841 ctgcagttct tattcataaa gcaattgggg acaacttaac ttgtatattt gttgaccatg
    27901 gtttacttag aaaaaatgaa ggaaatgatg tagaaagaat atttagagag aaatttgata
    27961 taaatttaat aagagttaat gcagaagata gattcttatc taaattaaaa ggagtttctg
    28021 aaccagaagc taagagaaaa ataataggtg aagaatttat aagagtattt gaagaagaat
    28081 caaataaatt aggaaaaatg gatttcttag tacaaggaac aatctatcca gatgttatag
    28141 aaagtggaca tggaaatgct gctactataa agtctcatca taatgttggt ggaatcccag
    28201 aagatgtaga cttccaagaa attgtagaac cattaagaga gttatttaag gatgaagtta
    28261 gaaaaatagg attagagctt ggtatagaag aaggattaat atttagacat cctttcccag
    28321 gaccaggtct aggaataaga gttataggtg atgtaactaa agaaaaatgt gatatattaa
    28381 gagaagctga tgctgtttat atggacgagc ttagaaaagc aggtttatac agagaaatat
    28441 ggcaagcttt tgcaacatta ccagatgtta agactgttgg agttatgggt gatgaaagaa
    28501 catatgctta tttagttggt ttaagagcag ttacttcttc tgatggaatg acttctgact
    28561 ggtataagat gccttatgat gtattagaaa gaatttctaa tagaataata aatgaagttg
    28621 atggagtaaa tagagtagtt tatgatataa cttctaaacc tcctggaact attgaatggg
    28681 aataaaatat agaatgccaa aaatccacat aaatacagga tttaaggtct ctcttacttc
    28741 tatgttatac tggtttgata ctatcaatat ttgccagtat cttatggatt aataagtaaa
    28801 aaacatcctg ctgaatgata aattcagcgg ggtgttttta ttttgtttta gaactccttt
    28861 gctctgaaac tcgaagtatt taacactgat gattttgtta tcttttagtt ccttttgtgt
    28921 catgctctga aagacaaatt gatacaataa aaaagtaatt tcttaatcat tgatggagat
    28981 atgtatttat gttactcttg atggtaattt gagtaatatt gttcatagct ttttatattc
    29041 aattaattag aaaaccattt ggtataatca taaataaact tgatgatata atagaaaaga
    29101 tttataatta ttagaagtat ataaaagtat tttatgtcta tgaaatgtat gtctataaaa
    29161 catatagatg acttttgtat attaatgtat gaatattgat attatagaag aattgagttg
    29221 tcaatgatac taaatcaaat atttatagaa gctggaggga ttatgagaaa taaaataatt
    29281 ataggaatta cttttttact tgtattacta atgtctggat gtgcttcaaa acaaaccgca
    29341 gaaataaacg tattagcaac tactgattta catggagtta taccttatga gttaacttct
    29401 tatgtaaaag aagaaagaaa aaaagataaa aatctaactt tagtagatgc aggagatttt
    29461 tttgatagtg gagaagtgtt tggttctagc atggatagtt attttagtgc tagatataaa
    29521 gatggtgata ataacactga aagttatatt gagacgccta tttcaaaaga tatgaaagag
    29581 gtaggttatg atgttgcagt tcttgggaat catgaattta tatccaacaa taaattttat
    29641 ctagataata tgatatctga ttttgagaag caaaaaataa atattttatc ggcaaatacg
    29701 tataaaaaaa atggagacag ttatacaaaa ccatacataa taaaaaatat taatacacct
    29761 gaaggaaacg taaaactagg aattttaggg cttactataa aagaagttgg tgagaggaaa
    29821 cagtggaaag atggaaaact tgttgactct aagtctttag aactaaaaga ccaagagggc
    29881 tacaatggtg aattgtatat gaatgattta gtagaagatg ctaaaaagtg ggttagtata
    29941 atggaaaaag agaagcctga cattatagtt gctgtggcac atacaggtga gaaacctaag
    30001 aaacctaaaa acccaggaaa tagaatacaa gacttagctc aacaagttga tggaattgat
    30061 gctatagttg caggtcataa tcatgttcaa attgagcaac atgattataa aaataaatca
    30121 ggagaaaatg ttatagttac tgagccagga aagcatgggg aatgtatttc taagattaat
    30181 tttaaattag aaaaaaacaa tgataaatgg aatatagttg acaaatctag taaattaatt
    30241 aaatttgaaa aaaatcaaga aagtgataaa tttggagaat ttatttcttc aattaataaa
    30301 gtaagtaaag ataagaaaga aataagatta tcagaagtaa agactcctga atgggataaa
    30361 atatatgttt ttaaatctgg aacatctgca gatgatatat ataaaacaat aggatataaa
    30421 tggagaaata tatcatctcc aaagagtgat ggcatggtgc agattgttgt aatgaataat
    30481 aataagcctg ttagctatat atatggtgaa gctgaagtta tgccaattaa tataaaatgt
    30541 gatgaatcag agtataaaga taatgttgta actatttatc ctaataaaaa tgataaattt
    30601 aaaattgaaa aaggtaaaca agaatatagt attaatttaa aacatataca atagaaactt
    30661 ttatcttatt taagtttaga ttgtacttaa aataaagtgt gtattatttt aaaaattgat
    30721 gataattaaa atcacaacat taaggaatag tacacacttt ttatgctaaa aaatagaatg
    30781 ttaaaaaatt aaacctttaa attaaagtac attgcttata ttatccaata ttatcttcaa
    30841 aggttgtgta atatttttat ccttatgtaa tattgctaac attttatttt ccatatctat
    30901 cccatctaat tcaatcaatc gcaatttttt atgtaagagt gaatctaata ataaattttc
    30961 tggcaaaatg gttataccta accctgcttg tgcagcttta ataagtgcaa aagaattaac
    31021 actttcccaa acaggataag ctttttggtt tgaaagagat aatacactgt ccaaagtatc
    31081 gcgaatggca cttccttgct cacgtaacaa taacggaaac gtgcataact gtcgtagaga
    31141 taaggcttgt ttagtattgg tggtggttat gcaatcatac ctttaatcca agagaaagta
    31201 gtaaatcaaa ctggatggat aagtgagaaa atatttacag atattataac tatttcacaa
    31261 atgacaccag ggccactggc tgttaatact tctacttttg ttggacttca aattggaggg
    31321 ttttggggag cagttgcagc cactttaggt tgtgtattat gtggtgtagt aatatctcta
    31381 tttttatata atttttttca aaagcatcag aactctactt atatatttga aatattaaat
    31441 ggtctaaaat cagcttcatt aggtttaatt atctcagctg cggctacaat tataattctt
    31501 gcattatttg ggacgaataa agtgtacttc aattctatat ttgagatgct aaactggact
    31561 gcactaataa tcttttttgt tacactactg atagtacgta agtggaaaat taatcctatt
    31621 gtgattataa taatgagtgg tatactggga atattttttt atttataagt ttttgctaag
    31681 taaaggatat ctattttttt atatcttaaa tccaaatagc caataaagta aggctatttg
    31741 gatttttaat aaattctgaa aagataatca gaaacctata taacatttaa attatatcac
    31801 tggttttcta caaaccaatc ataagcttct tggcaaaatt tcttactatc tatatagtaa
    31861 ctattatgaa cattattaat ttcaacaagt ttctttttac attttagtga attataatag
    31921 ctatcaaaat attgggtttt aaaatatcta tcatgaagtg ttttaaagac aagagtaggt
    31981 atgttgactc catcttttat cttactatct tttgcaataa agcattctaa cattgtatta
    32041 attatctttg tactattttt ccaaatatga ggatttttct tttcaaatat tttataaaat
    32101 tcattatcat agacttcatt ataaccttta tatttaggtg gttttactgt tatgtgaaaa
    32161 ttaggaagaa acttaaagaa aaaatataat aatttttatg ttctgtttta tatattattc
    32221 atattttatc acagataata tactaaagtt aattttttta ttgtacaact attataataa
    32281 ttcttctata atataagaca ttctataatt ttatatttaa gttcatgtgt atttaattgt
    32341 ttttatataa aatgtccagt aatactattt ggatttattt ttatttcatc aggagttcca
    32401 gaagcaacaa tttcccctcc atttattcca ctgtctggac ctatatcgat aatccaatca
    32461 gaagcacgta taacctctgt attgtgttca ataattaaaa gtgaatgctg ttcatttaca
    32521 agttttctaa aagtaattaa caaattttct atatctttaa aatgaagtcc agtagtaggc
    32581 tcatccaata tgtacagcat tcgtttttta gtttttgtcc ctaattcttt tgctaatttt
    32641 atcctttgag attctcctcc acttaatcct aatgtatttt gtccaagctt tagatatcca
    32701 agtcccactt catctaataa ggagagtttt ctatatatat cagagcaatc gctaaaaaat
    32761 gtttttgctt ctgctacttc catgtcaagt atatctgcaa ttgtctttcc cttgtattgt
    32821 atagacagta tttctttttg atatctctta ccttgacatt cactacaagt tacccattgg
    32881 tctgccatat attgcagaat aatttttgtt ttacctaatc cttgacagtt tgggcattgc
    32941 ccctttttgc tattaaagct aaaatagctt tcatccaata atttttgttt tgcttgtgga
    33001 gtttcagcaa atagagaacg aatttcatca aaaatattga tatatgtagc aggtgtagaa
    33061 cggctagatt ttccaatagg tgcttggtcc attaagataa aatcatcaat agcatcaaat
    33121 ccagtaaatt cagtataatt tttatctggg atagattttt gtttcaattt ttcttctaga
    33181 gcaggaagta aactgtgaaa tacaagagag ctcttaccag aaccactaac acctgtaata
    33241 gagcacatac agtttaaggg aatttctaca tcaatatttt ttaagttatt tgcatggcat
    33301 ccttttaagc ttaaccattt acttggtata attttatttt gtgaacttat tttattgtat
    33361 gtacttaaat atttacctgt tatagagttt ggatttttta taatttcttg ggttgtacct
    33421 tctgcgataa tgaacccccc ttttgtacca gcacttgggc ctacatcaat aatataatca
    33481 gcagacagta ttgtatcttt atcgtgttcc acaactataa cagtattacc cttatctctt
    33541 agctcttcta tcattttgat gattaaatta tggtctcttg ggtgtaaacc aatagatggt
    33601 tcatctaaaa tgtaggttaa gcctactaat tcacttccta gctgacttga taatcgtact
    33661 ctctgcagtt ctcctccaga taaagttgga gctgttctat ctgttgatat atagctaaga
    33721 cctaccttta atagataact aactcgaatt tttaattctg ttaagatatc atttaccaaa
    33781 gagagctcat ttttttgaag gtgatttggt aattcacaca accaattcca taactgttct
    33841 atcgtcattt ttgttagttc agggaagcga taacctttga ttgtagtgaa tctagcctca
    33901 atgcacagca attcgccacc acatgtattg caaggaattt tgttcatata ttgatgcaag
    33961 gtattatttt cagaactaga ttctctaaaa agtctttgaa tgtggttgat tgctcctgaa
    34021 gcaggtcttc taatctttga ttctcgtccc tttgattcaa agctaaattc atatatctta
    34081 tcgtctgtac catataagat tgcatctata aaatctcttg gtaattcatt ccaaggtata
    34141 tctaggtcta tatttttatc ttttgctatt gcatataatt cacctaccat ccaattacca
    34201 tttggctttt tccccctcaa ttttccaaaa tagtaagtag ctccatctaa tattgaaata
    34261 gttgggtctt ttacaattaa ttgaggatta acttgatagg taaaacctaa tccattacaa
    34321 tcgtgacaag ctccagtatg ggagttagca ttgaaggttg aagtatttat cttagaaaaa
    34381 atagtttgac aatgtggaca ataatttttt gtattattct ttttttctaa tggaattttg
    34441 caatatggac agtaactttt tccgattgat acaaataaca ttcttaaata atcccaaatg
    34501 cttgtaagag taccaacagt agagcgagga ttttggcttc cttttttctg ttcgatggct
    34561 atagtagggg ttaatccctg aagtaaatca aattccggtt tttgaagctg ttctgaaagt
    34621 tttgctccgt tacttagatt ttcaaaataa cgtctttttc cttcttcata aatcgtatcg
    34681 aaagctaaag aagattttcc actaccactt acacctgtaa taacagttat ttgatttttt
    34741 ggaattgtaa cttcaacatc ctttaaattg ttttgccttc ctccttttaa atagatagag
    34801 cttttattat gacaaagtga atttttaatt gatttattta ttgcttttgt aaatttgaat
    34861 ttcttttcta gttcaagagg attgcctaat tcttttattt ccgaaatacc taatattttt
    34921 aactgagaaa agattgattt caaataatct tcaggcaatt tttctattaa caaattaagg
    34981 gcttctattt ttatatttcc gcttgataat atctctgcca tttcatctaa taaatttttt
    35041 tctgctcttg ttaattgatt gtattttttg gaacttaatt ctgtccataa atcccatgga
    35101 agcaattcta aaccaatcat attattaaag tcatgtagaa gattaccctt tacagaatgc
    35161 caacctttcc aatttttatt atatccaaag tcatctggat gagcatgtcc ttgccgacat
    35221 cttatccagg cttctgaacc agttaaaaaa ccatctgttt ttccgacatc atgagtgtca
    35281 aaattaattt gaaaataatt tttttgtatg tcatctattt gtgcatctat tcttatccat
    35341 ctctttgtta gagtatcata gtattctaca agccagtggt cttcataagt aagctcactt
    35401 tcaaaataat tagcaaaacc tgctctcatt ctagcctcaa aacctcgata tcttaggagc
    35461 gatacaagaa gtaaagaata atctctgcac attccaacaa gcctttgttt tgggtttctt
    35521 ggaatagtta gtgggttaga atttatttca aatattcttt ttaacatttg aggaattgta
    35581 cgaagcaatt cctcatcaga ttgttgctta ttaaaggata taccatataa ttttccttgg
    35641 tctccatgaa ttactaggct ttgaaccact tttaataact caattggagt ctttggtaaa
    35701 tcttgatatt tattttcaaa ttttttagga tttgtgatga tactttgatt tttatagtta
    35761 tttattttca tttatatttc acctctctaa atggatgata taccctgaca tggtgtcata
    35821 tgcaaggaca atttagattt ttggccaaaa tttaaaaaat aaataaaaaa tgatacagtg
    35881 ataaattgta atacaaatat agtttcaaat tgcatataat ataattattt aataaaacca
    35941 ttatctattg aatgttaatg aaatgttaag atggcattct tgaatattaa gaaatagaat
    36001 actataatat agtgtgaagg atttttagta aaaaaatttg taagtgttaa tatacattaa
    36061 aaaataggtt tataatttgg aggaaaaaaa tgaaattatc aaggtataag aaaaatagtt
    36121 tagttctgtc tataatttta tgttcactta ttatttcaat ccttgtaatt actgttttta
    36181 ataccgtaaa actacacaat atatctaata aacaatctaa aaactattta aatgatgtat
    36241 ctacacagat tgtaatgaat gttgattcta aaatcaaatt tattttatcc gacttaagaa
    36301 taatggcaga ttttattaag caatatgaag gtgatagtcg atatgagtat ttatctaaaa
    36361 gaaagactag ttataagtat tatgatgttg gaataataga tttagaaggt aaagctgagt
    36421 ttttaagtgg aaaaaagttt gacttaaaga atactgtttc ctataaaaaa ggaattgaag
    36481 gtaaagaata ctcagaagta atagagtcaa tgggttttgt tctttatagt gtacctatat
    36541 ttaatgataa tcatgaagta tctagtattt taataggtgt atccaataaa gaaagtatga
    36601 atgagattct taatatagat aactttaatg gaaaaggaac tattgaaatt attaatagta
    36661 agggaaaacc tcttttcata ggaaaaaata ctgaattaat aaaggacctt agccataaat
    36721 ataagagttc tggagatgaa ccttgggcac aaaagatgct tgaggatttt aaaaatgata
    36781 aatcagggaa tattacaata acctcatcaa aaggtgtaca atgcttatta acttatcatc
    36841 caattacaag aggtttaaat gattggcatt ttcttctcat agtacctgaa gatgctgtac
    36901 ttggtgaact taataaatta aactctttta caataacaat gacatttata attacatgta
    36961 ttattggaat tataacatta attttatata ttataagaag aaaatatgta aatcaaatag
    37021 aaaatattgc atatttagat agtataacaa atggtataaa ttcaactaag tttagtatgc
    37081 tagtcaaacc tttaatttca gaatcacctg atagtactta tatgatgata gcaatgaata
    37141 taaaagattt taagctaata aatgattgtt tcggtagtga aaagggaaat atgacactaa
    37201 aacatcttta caatattttg tataaaaaca ttgatgaaaa tgaagaattt gtgtgccgtc
    37261 atgatgcaga ccttttctat ttgcttatta agaatagacc agtgttagaa gccctggagt
    37321 tattatataa aatagaagct gacacaaatt attttaataa aaatagggaa aatccatact
    37381 ttttaagact atcagtaggt atttacacaa ttgaaaatca taatgaagat ttaattacaa
    37441 tacaagacca tgcaaataca gcaagaaaaa gctttaataa aactcatcaa agtgattttt
    37501 ctttttatag tgatatagaa agaaaacgat taatatctga aaaggaaatt tcaaatttaa
    37561 tggaaaaagc ccttgaaaat aaagaatttt ttatgtgctt acaacctaaa attgatataa
    37621 aaagtggaaa aattagtgga gcagagtctc ttgtgcgttg gaaaaattca gaaaagggca
    37681 taatttatcc aagtgatttt attcctcttt tcgaaaaaag tggatttata tgtaagttag
    37741 atttatatat actagaagaa acatgcaaat tgatttctaa gtggataaaa gaaggtaaag
    37801 aagtgttgag aatttctgtt aatgtatcac gtcaacatct gaatgataaa ttgtttttag
    37861 aaaaatataa gcatatatgc aataaataca atgtaccaac atgtctgata gatttagaac
    37921 ttaccgaatc tattttctta gaaaatccag aagctataga tataatagag gatattcatt
    37981 ctaatggatt taaatgttct atagacgatt ttggatttgg gtactcatca ttaggaatat
    38041 taaaggattt taaagttgac ataattaagt tagaccgttc attctttgtt agcaaaaata
    38101 atatagaccg tggtaaggta gttattaagt ctattattga acttagcaaa aggctaggta
    38161 tgaaagtaac tgctgaagga attgaagaat tagaacaagt tgagtttcta aaaaatgtgg
    38221 gatgtgatta tattcaaggt tatgtttttt caaaaccatt attaatttct gattttgaaa
    38281 attttgcata tgaaaataat aaaattaaaa atatccatta ttaaatgtac aagataaaaa
    38341 atttaggcaa gttatataat taaaaatata acttgtcttt tttatttata tatttaataa
    38401 aaatatattt gcagattttg taaaaataat tttcaaatgt tgtaagttgt ggctaaagct
    38461 ttattgtatt attctgaata tagaaagata gttactgtct tagataaaaa ctgaaacata
    38521 cataataata taaaatttgc tatattaata actctaatta ttttggaagg agggaaaata
    38581 tgataagtgg gagaatgtta ctcatcatga attttctaaa aggaaaaaat aagacaagtt
    38641 acaaggatat tagcaaacaa cttagtatag aagaaagaaa agttagatat gatataaata
    38701 atttaaatat tgttttgcgt tcattaaaca agtcagtaat acaaaagatg aacaaaggtg
    38761 tgttaattat tcctgatgac ttccaagtag tatgtataaa taataacgaa tatgtatttt
    38821 ccttaactga gcgagtatct attatgaaaa ttatatcaat gtttaaaata gataatctaa
    38881 acttagaaaa attaagtaaa gaatttcagg tttctagaag ttcaattaaa aatgacctta
    38941 atgttttgtc aagtgaatta gaaagaaacc aaataactat tacttatgat aaatctttta
    39001 aaataagtgg agaagaagac attatatata agcaaagatt gaacatatta aaatcgtatt
    39061 cttatctttt tggtaaggaa aaatctgatt taagtgtatt tgagaagtat ttagtgaatg
    39121 taattgaaaa aatattcaaa gggatttatt tgattgatat atataattgg tctatgaatt
    39181 tattattgca aatgggatgg actttaaatg atgaatcttt tacatggtat gtgtcaaaca
    39241 ttttcctttt tacatggtat atacattcag atgaaaaaca tcctttagaa tctgcttcat
    39301 taagtgagcc gtgttttgaa aataagtttg accaagaatt tgaaacaatt attcaaaaaa
    39361 agttgactaa ggaacaaatt tttctgttaa agagttttgt attttttaca aataaatatg
    39421 caagtttaaa cgaggatatg gatttgatta gtacagaaat tatagttcaa gatctaattt
    39481 acaatatgtc agaaatatta gagattagct ttaaagaaga tatgattctt tataaaggat
    39541 tactcaatca tattgcccca ttgatagaga gaatcaaaca gaatatacag atttatgagg
    39601 aattacttca tgtaatacca gataaatatc aatatgtact tgaagctact aaggtttcta
    39661 ttcaaaaaaa cagagtttta agtcaaataa agaatgaaaa tgaaacaata cttttggcaa
    39721 tacatttctt aggaagtata catagaaatg agaggaataa ctacaaaaat atattgttag
    39781 tttgtggatt tggatatggt gtaattgcta tgttaaagga tagtttagta aatgattacc
    39841 agataaatat agtagagagt attccatcat ataagttaca agattataaa gaatgggaaa
    39901 gtattgactt cattataaca acatcaaaga tagtaataga cttgcctaaa acaattattg
    39961 agataaatcc atttttacaa gaagaggatt atttgaagtt tgaagaaaaa gggataaaaa
    40021 gaaagaacgt cttaactaat tatatgtcaa taaaaaaaag gttggacttt ttagaagaag
    40081 atgcccaaaa aagagtatta agtattatat gtcaagaact tggatacagt gatgagagaa
    40141 tattatttag acaacttaat ttaagtgatt taataggaat tgataccata aggataattg
    40201 atggagatat gaaatgggaa gatgctgtaa aaattagttc aaacatactt gctaattgtg
    40261 attttgtatc agcacaatat gctgagaata ttatagatat tttaaaaaat gtgggttttt
    40321 atgctgtaaa agataacgaa tttgctttac tacatggcaa tgatagttca ttagtcaaag
    40381 taagttcgat aagtttatta atatgtaaag aagcagttag ttttggagat aaaaaggtta
    40441 aaatcatatt ttgcctagca agcagagata aaaaagagca aattcctgca ataattaatc
    40501 taactaaaat ggtttataag actaatttta ttgctatgtt agaaagtgca aagacaaaag
    40561 aagaagcaga agttttaatt cataaatatg agaaggaggt cactgtatga ataaattagt
    40621 taatgtaaaa tgttcattta aaatacatgt taattcaaaa gaagaagcca ttatatcgtt
    40681 agttgatgta gtgtcaaaag aaggatattt aatagataaa aatcaatttt taaaagatgt
    40741 tttaaagaga gaagaaacat tgtcgactta tattggacat ggtataggtc ttcctcatag
    40801 tcaaagtgta ggtgttaaaa actcttgtat tacaatagga aagcttgata ctcctattga
    40861 atggacagaa gaaggagaaa aagtggattt aatatttttg atatcagtaa caaaagacaa
    40921 tgaaaataat cttcatttaa agattttatc aaaattagca cgtttattga tgcatgaaag
    40981 ttttagaaat caaattagat gttcagatga acaaactgtt tataatctta taaaagaaaa
    41041 aatagaggag gaagattaaa atgagaattt taaagttttt gcaaaaacat cttatgacag
    41101 caacatctta tatgattcca tttgtggttg caggaggaat attatttgct ttatcagtaa
    41161 ctttaagtgg acaagctgct gttcctgaaa caggctggtt ggctaaactg aatcaaattg
    41221 gagctgctgg cttggcttta ttcattccta tattaggtgg atatatagca tttagtatgg
    41281 cagataaacc aggattagct ccaggtatga taggtgcata tttagcaaaa gaagtaaatg
    41341 caggttttat aggtggtatc atagcaggat ttatagcagg atttgttgtt ttacaattaa
    41401 agaaaattaa attagctcct acaatgagaa ctttaggttc tatatttatt tatcctttag
    41461 ttggtacatt gattacaggt ggtataattg tatttttaat tggagaacct atagcttctt
    41521 ttatgacatg gatgacaaac tggttaaatg gaatgtctgg tgtttctaaa attcctttag
    41581 gtggaatctt aggaggaatg atagcctcag atatgggtgg ccctataaat aaggttgctg
    41641 ctacttttgc ccaaactcaa gttgacacac ttccatattt aatgggtgga gttggcgttg
    41701 caatttgtat acctccaatt ggtttaggat tagctacatt attgtttcca aaaaaattta
    41761 gcaaggaaga aagagattca ggtaaagctt cactattaat gggatgtgtt ggaattacag
    41821 aaggagctat tccatttgca acatcagatc cagttagagt aataccttgt attatggctg
    41881 ggtcgattgt aggaaatatt atggcttttt tattaggatg tttaaatcat gcaccctggg
    41941 gaggtctgat agtgttacct gtagttgaca ataggttggg gtatattgca tcagtattaa
    42001 ctggagcagt agttgttgcg gtattaatga aattagtaaa aaaagacgtg aaagaagatg
    42061 aagaaattga agaagattta gatgaatcaa ttgaattaat atttgaagaa ttataaatat
    42121 aaatggaagg aatttgtata aaaaagtaaa tataaaacta taaatttaaa ggagataaga
    42181 atatgaaaat tgtagcagta acatcttgtc ctagtggagt agcacatact tacatgtcgg
    42241 ctgaatcact tgaattatca gccaaaaaat ttggtataga aataaaggtt gagactcaag
    42301 gaagctctgg aatagataat gaattgtcac ttaaagatat tgatgaagca acttgtgtaa
    42361 tattgactaa tgatgttgag attagaaata tggaacgttt taaaggaaag aaggtagtaa
    42421 gaatgagtgt atctgagata attaagaaat ctgatgcatt aattaagaaa ataaaagata
    42481 cttttcaata aaaattgatt tattttacat ataactcaaa gatgttttta aagaaatttt
    42541 tattaaaagc atctttgaaa atatcttaaa aatagaataa aaggagatat agtcatgaaa
    42601 aagttaccaa taaagactgt cgtgcaaaga ttactaaact tacaagaaga ggggaaaagt
    42661 gcaactcttt taggaattgg tcctatgtca ccaaacttgt tacaagcaag ttttgaacta
    42721 gcaaaagatg atgattttcc tttaatgttt atcgcaagta gaaatcaagt agatgcagat
    42781 gaactaggtg gcggttatgt aaatggttgg aatcaggaaa cttttacaaa agatattaag
    42841 aaggttgccg acaaagttgg atttgatggt ttatattatt tatgtagaga ccatggtggg
    42901 ccatggcaaa gagataaaga gagaaatgac catttgcctg ttgatgaagc aatggagtta
    42961 ggaaaaaaat cttatttagc ggacattgaa gcaggttttg acctattgat gattgaccct
    43021 acaaaagacc catttgaaat tggaaaagta attccactag atgtagtact agaaagaact
    43081 gtagaattaa ttgagtattg tgaaaacgag agaaaaagac ttaatctacc agacataggt
    43141 tatgaggttg gtacagagga aaccaatgga ggtctgactt caactgagac gtatgaaaca
    43201 tttataacta gactaaaggt tgaactcgat aatagaggtt tgccaatgcc aacatttata
    43261 gttggtcaaa ctggtacttt aactagaaag acagaacaag taggaacttt taattttaga
    43321 aatgcttatg atttagctca aatggctaag aaatatggag ttggtttaaa agagcataat
    43381 ggcgattact tagatgatgt aactttatta gaacatatac catctcaaat tattgctaca
    43441 aatgtagctc ctcaatatgg tactgaagaa actagggcat atttaaaatt agcagaagtt
    43501 gagtgtaaat tagaaaaaga gggcttagtt gagaaaactt ctaatatcag acatgttctt
    43561 cttgttaatg caattgaatg tggacgttgg agaaaatggg tagtaggaga acaaaaaaat
    43621 ctaactactg aagagatatt taaggatgaa gttttatcta atgaaatact agatattgca
    43681 ggacattata catttaataa tgatgatgtt aaaaaagaaa tagaagtgct ttatgataat
    43741 ttaagtaaga ataatattga tggtcaaaga tttgttgtag accatattaa acgtcctttg
    43801 cgtgattatg cagaatgcta taacttaaaa ggtgttacta cgaggatttt aaataaataa
    43861 taggacttat agtggtgagt attgttatac taggtattta tagttataat tatacttacc
    43921 actattaaat ttaggaggac taatattttg attaatttaa tttttgatgt agatgatact
    43981 ttatataatc aattaacgcc tttttacact gcatataata aagtgttttc ttcaataaag
    44041 gatatttcta ttgaagattt atatatgagt agtagaaagt atagtgatga ggtatttcat
    44101 atgactgaaa atggagaaat gccaatcaaa gaaatgcata tttataggat aatgaaagct
    44161 tttgaggagt tgggaaattc tattacagaa aaagatgcac aaagttttca agatgagtat
    44221 atatatcaac aatcacaaat tacactaata ccagaagttg agaggatttt aaatttttct
    44281 aaagaaagaa atattaatct aggaattata actaatggcc catcaaacca tcaaagaatg
    44341 aagttaaagc agttaaatat tgaaaactgg gttgataaaa gtaatatttt tatttctagt
    44401 gaggttggat tttctaagcc agatactaat atttttagag tagctgagaa tgttatgaat
    44461 ttagatagag aaaatacata ttatgtgggt gattcttata gaaatgatgt attaggtgct
    44521 aaaaaagctg gttggaagag tatatggttg aaccataggg gacatgagtt agaagagttg
    44581 ttttataaac ctgattttgt aattttggaa cataaggatt tgatatcttt atttataaaa
    44641 atatgtagtt ataaaaatat gtagtaaaaa aatgttaata atagaattgt ataaaataat
    44701 taataataac tatataaaat tatatgttta gattgtgagc aatctatttt atgttaaaaa
    44761 tagattattt agaagaaggg gaaatcttaa aaaaatttga atatcccctt tttattattt
    44821 aattaaaatg aaatagttag aaatatattt tatttagttt agtcaataac attttgggat
    44881 tctttataac tacatgttaa ctgttgaata ggtttatcat atggtttaac ccatccttta
    44941 ttaatactta attctgtaag tgctgtgtga ccttctacca tttgagttaa tgaagctgaa
    45001 tatatagctc ttagttcagg tgtactactt gtcaatgcag aattaagata catattggca
    45061 gatgctttag cagatgaaag catacttaac attattactt tatcgtctat ataagtattg
    45121 ttcttaatta gattttctat catatttctc atgttatcta tacctcctca gaaattagta
    45181 tttcattttc aactataaat tgttgtatag ctttaattct tccttctgtt gctaatatac
    45241 tagattctga ttgtcttttt agatcttcat ctgatattaa catatttatt gctctttgca
    45301 taagtaatcc gtcagactcc atttttataa ttgctgctaa agatattttt tcagtttctg
    45361 aaagtattct catttaatca tctcctatat tgtaatttat gctttaatag tatgtctttc
    45421 ttttcaagga aataaccatt acactttttt acagtatttt atattctcgt tatatcaaaa
    45481 tttagtcgac ttaatatatt aatataattt tgtaggatat ttatcattaa atatctataa
    45541 aataaattgt ttgaatattc ataaagcaaa atttatttgt atgtacttct tcaagagtaa
    45601 atatgagttt tttcaaaaaa ttaattttta tgataattat taagggctat atttttctta
    45661 aagttgtgat tatcattatt aatttacaat aaaattgccg aaaagccctc tcctttaggt
    45721 gtagggatga aaggtaaggt cttaatataa aaaagttaga ataatgcttg taatctaata
    45781 ccgcta
//
LOCUS       DAYAPA010000017        43920 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.017, whole genome shotgun sequence.
ACCESSION   DAYAPA010000017 DAYAPA010000000
VERSION     DAYAPA010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 43920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43920
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.017"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            135..485
                     /locus_tag="ACGI59_000864"
     CDS             135..485
                     /locus_tag="ACGI59_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFG9738669.1"
                     /translation="MSAKVTNQYGNIEIDNQVIAQVVYRAAMESYGLVGLAYKSKGIV
                     ELLKGENATKGVRVEEVEEDAIAIELYVIIQYGTNISTVANNIIDRVKYVVEKMTGVR
                     VTRIDINVQGIRVK"
     gene            504..2147
                     /locus_tag="ACGI59_000865"
     CDS             504..2147
                     /locus_tag="ACGI59_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HFG9738670.1"
                     /translation="MIQYIDGKRLREMFISGANNLQNNKELVDKLNVFPVPDGDTGTN
                     MSLTISYALKELAKVENDNISDIGKALSKGSLMGARGNSGVILSQIIRGIAKSIEGKS
                     KLSTEDLAKAFKNGSDTAYKAVIKPIEGTILTVVRESGEFAIKTAKKEKDVVKFLSML
                     VKESNSSLERTPDLLKNLKEAGVVDSGGKGLVLIYEGMLASIKGDNIEIKNVDLDTNI
                     STSMDFAKSTTSTDDIKYCYCTEFILESSKVEDTKIRDIMMAYGDSLAVVGDDGVIKV
                     HVHTNDPGNVLQEALKYGQLLTIKIENMKLQHENTLLDVEEKKENDSEPLEEEKEFGF
                     IATSMGEGLANIFKDFGVDHIIEGGQTMNPSTEDFMNAIKHINAKNIFIFPNNSNIIM
                     AANQAKELSDKNIIVIPTKNTPQGFAALVTFNGELSEDENKEAMMNALNSVKSGQVTF
                     AVRDTVMNEIDVKEGNIIGIAEGNLLSAGDYVDEVTSNLIEKLVDEDTAIITLFFGED
                     VTESQANELRTSLEEKFEDVDVELYYGGQPLYYYLISVE"
     gene            2228..4285
                     /gene="recG"
                     /locus_tag="ACGI59_000866"
     CDS             2228..4285
                     /gene="recG"
                     /locus_tag="ACGI59_000866"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFG9738671.1"
                     /translation="MDLYKDVQYVKGIGPKKADKLNKLGIFTLKDLLYYFPRQFEDRN
                     NLKKIAQLEDGEKVTIKAVISSINTFSPKEGMTLTKIDVKDETGSAKLVFFNKSYIKN
                     TFRPGDSILVFGKVKKKFNNLELTSCELEYLTNSPKNTCRFMPVYQLTYGVTNKEIMS
                     IIRTVLEDKELIIQEYMPQRIIEKYRLCSIDFAVRNIHSPSSKESLKIALYRIVFEEL
                     LILQLGLFVFKSGRNKEDGIKFETSKDLKKIISALPFKLTKAQNRALDEIIQDMNLEK
                     IMNRLVQGDVGSGKTVVALLALANCVLNGYQGALMAPTEILAGQHYISLTESLKDFGI
                     NVGLLIGSLTKKQKDTVLEQIKNNEIDILIGTHALIEDKVEFNNIGLVITDEQHRFGV
                     MQRSKLSLKGANPDILVMTATPIPRTLALILYGDLDISIIDELPPGRQPIETIAIEKS
                     KRDRAYNNLVRREVESGRQVYIVCPLVEESEAIEAKSAVELVEELRAEYFHDLRLGLL
                     HGKMKSSEKDEVMRLFKDKEIDILVSTTVIEVGVNVPNATLMIIENAERFGLAQLHQL
                     RGRVGRGSHKSYCVLIYDSKTDVCRQRMAIMEETNDGFKISEKDLEIRGPGEFFGTRQ
                     HGLPELKVANLFKHIKILKLAQQEARYILGEDNNLQLKENMALKKEIIDKFKDTLKEI
                     SLN"
     gene            4344..4898
                     /gene="rsmD"
                     /locus_tag="ACGI59_000867"
     CDS             4344..4898
                     /gene="rsmD"
                     /locus_tag="ACGI59_000867"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HFG9738672.1"
                     /translation="MRVISGKARGLKLNTPKNEDVRPTTDRVKESLFNMINSYIMESE
                     VLDLFAGTGSLGIECLSRGAKSCTFVDISKESIDIVKSNIKKARVESESIILNLDFKT
                     AIDKLKLQNSKFDIIFMDPPYYKNMFIEAIEKIDNSNLLNEDGIIVVEHDTNDLFPDK
                     ICKLEKTRDKKYGNTTLTFYKMEA"
     gene            4900..5397
                     /gene="coaD"
                     /locus_tag="ACGI59_000868"
     CDS             4900..5397
                     /gene="coaD"
                     /locus_tag="ACGI59_000868"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFG9738673.1"
                     /translation="MENKPRKAIFAGSFDPITNGHLDIICRASKLFDELQIGVLNNPN
                     KKGLFSFDERVKLIEKSTSHLNNIKVVTFDGLLINYCQENGIGALVRGVRSGADVDYE
                     LQMAHMNRELNPDIETIILPSCTKYSFISSSLIKEVLLFDADIKNLVPKIVLEELKKK
                     TSGGN"
     gene            5401..5925
                     /locus_tag="ACGI59_000869"
     CDS             5401..5925
                     /locus_tag="ACGI59_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9738674.1"
                     /translation="MKIDLEMIELFEQLEEIFRSASTIPFSHKCTVDKDEVLAIINDI
                     RTLIPEEVTQAVWINKERNKIISQAKQDATNIVEQAKKEAERIQQEYQDNIEELKKNS
                     EDVVKAYVESSEPVVQADQRAKDIVDRAERIANEIRIGSIEYAEDVLSSVEYNLREIL
                     EEVKRDKAELRPKK"
     gene            6269..8188
                     /locus_tag="ACGI59_000870"
     CDS             6269..8188
                     /locus_tag="ACGI59_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFG9738675.1"
                     /translation="MKERAKKILDFIIKNNNVTSQELQEKFNVSKRTIYYDILAINEQ
                     LGKSGNIKNVKHKFIFEGNLCDARKIISTEEDKFLDSDYRKTFILNKILLGEKISIEK
                     LTNEMLLSKNTVVQTITDVKKYLQTMGLRLEYKGKYKIIGDEYVIRELFLIIVQENIL
                     EINSISEEVSSFDTKGHIKLTDYSLLNLTKFVEFLNKRIRDGKTLYSYKYLNEAKKIS
                     YFSNCKELLCEEANENEQAYICTYISSLPSLNSEVKEDVVEEYVDKLIDKFEVNTAIK
                     LESKHEFKKNILRHLHSSYNRIRFKFPIRNPMLDETKYKHESLYKIIKSIIENEEEFP
                     VFEGIREEEIGFIAAYFGGYLRGSRDNGLRRNKVLLVCPNGLMVSKSLEIQLYKYIPT
                     IEIVGIVSIKQLKEVNVYYDYIITTIDIQNVNNVIVVNPLLTSSDVQLLMNKLISVKE
                     NEKYFNLELIIQAIRKNGVINNEEALKADLLNIIHKIDEGEMYQPMLKELINAERVNI
                     IKSVRDWKEAIKIASKPLLEDNSIEELYIENMIKSVEKYGPYIVLADRFALPHASSKE
                     GVNKLAMSLLIVEDEVDLLGKPVNIFMVLAAVDNTTHIRALASLSEMMYEEENVKLII
                     NGDKSSILELINKQN"
     gene            8201..8488
                     /locus_tag="ACGI59_000871"
     CDS             8201..8488
                     /locus_tag="ACGI59_000871"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFG9738676.1"
                     /translation="MKILAVCGFGVGSSMVLKMTIQKALKQLGIECEVENTDINSARG
                     ADCDCIFTSQELLNELKGTCNVDVYPVKKYMDLAEVTEVVQTFLSDKNDKK"
     gene            8621..9880
                     /locus_tag="ACGI59_000872"
     CDS             8621..9880
                     /locus_tag="ACGI59_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFG9738677.1"
                     /translation="MLDFISNNILRNPSMLIGLIAAFGLVLQKKKASDVIKGSLLAAF
                     GIIIMETGTGMLVSSIAPINGAFQSISGGAVTKGLSDVTFTASYGGIIGLAMFAGLAI
                     HLMIARFTPIKTIFLTGHMLYWFPFIFVASGVEAGLNGGVLIAFAAVLSALYWSIMPW
                     LLRKYVFDVIGDDSFTLGHPSGCLALISGFVAKLVGNKEKSTEDINIPQSLSFFREIS
                     ISGGMAVFLMFIVVGIVIPGAFKDSNEFIGTYAIKQGLSFGAGLIIMLHGVRMLINQI
                     MPAFQGISEKVVPNALPAFDCPILFNYKPNAVLMGFLVAMLTSTAMILICNYFNVFNL
                     LLIPLVITSFFECGAAAVIGESQGGIRGAIIGTAIAAIFMVVLVGISAVIYSGTIQNW
                     ILIFGGNDLSLFGIISRFIGSLFGGIL"
     gene            9883..10623
                     /locus_tag="ACGI59_000873"
     CDS             9883..10623
                     /locus_tag="ACGI59_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFG9738678.1"
                     /translation="MKFKYIDEIRRLIDIIENEEKVNMEKAVQIFVEAIESKNAIFSF
                     GASHAGILTEELFYRAGGCVLINPVFAKSLMLDTAPITHTSKMERLEGYGTALADKTP
                     IKKGDVVIVHSVSGRNPVSIEFAMKAKENKAIVICITNLSYSKSVSSRHSSGKKLYEV
                     CDLVIDNHGQKGDACVEIDGLNQKVSPTSTVIATTVMNSIIAQATQELVNKGLKNPPI
                     FYSANIDGGDELNKKIFDEYKSVIHYEY"
     gene            10946..12256
                     /locus_tag="ACGI59_000874"
     CDS             10946..12256
                     /locus_tag="ACGI59_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HFG9738679.1"
                     /translation="MKKENKMEGIKVGMLKKLCSLAMVAIMTTTLITGCSSGNGKDSK
                     KDGEKEKLVLWMPPVENNMKEVWDPILDKFEEKNNCEVDLQIIPWDNYSEKFATAISA
                     GEGPDVGYMYAEMYPQFIESGAVEDLTNYATKEDKEQSIYIKTSEMMGGMYGMPFQAA
                     NPGVLYYNKDILEKLGEKPPKTWEDFKRICKKATQDTDGDGKIDQWGLAQGWGAKTFG
                     NLNWNWYPYLWQAGGDIFNDDLKSVKFNDKSGLEAAKFLKELQAYVPEDSLSKDSNEM
                     IESVFGPGKAAFTIMLSSAATSVFDESFPDLNWGFVTGLEDKKAATFGAVDHLSLISS
                     AKNKELAYKLIQHMLSVESMTEFHKAIPRAPITKGEPYQGDERFKEMVENDKNVYRPL
                     VVGPHGVEIYEYLWKELQTMISGDKTPKQALDDAAKYSNDLLAQ"
     gene            12354..13271
                     /locus_tag="ACGI59_000875"
     CDS             12354..13271
                     /locus_tag="ACGI59_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFG9738680.1"
                     /translation="MENLVREFEENSKRKKTKLNIKPYMYILPLGIILVAFYVIPIIM
                     SIYFSFTKYNIISPATFIGLENYKKLFTDETLKVSIINTIKFTVVVVPCQTILSLILA
                     VWITGKGNSKIASFAKGAIFIPVLSSMVLIGMVWRALLNGEGSIIYQVLGAFGIESSK
                     LLGDSKTALPTLMFISMWKNIGYFMVIYISAIMNLPKHCYEVAKVDGATKLQEFIKIT
                     VPLLKPTTIMVVFLGSIWSLQVFDLIYTVTGGGPGISTMSIVMHAFNLNFKNFNSGYA
                     MTVANVLFLLIAVVSILQNKLVKRDNSDF"
     gene            13287..14096
                     /locus_tag="ACGI59_000876"
     CDS             13287..14096
                     /locus_tag="ACGI59_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFG9738681.1"
                     /translation="MKTARRLLSDGLLLFIACASLVPFIYMLIISLKITYNSYSLDIS
                     FSTVTLQNYIDIFTKKGFAQYFFNTAIVSFSGVLLNLVFSTLAGYSFAKMDFKGSDKL
                     FLFMIMTLIIPSQVTMIPLYIIMKHLGWINSYLALIMPIPTAFGVFIMRQAILGVPKE
                     LLESAKIDGCSDFRILIQIVLPLIKPALITLAIFTFMGAWNEFMWPLIATTKDAMRTL
                     TVGLSTLKTFQITNYGQMMAGATITFLPPFIFYLILQSKFVEGVSLSGIKG"
     gene            14154..15902
                     /locus_tag="ACGI59_000877"
     CDS             14154..15902
                     /locus_tag="ACGI59_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HFG9738682.1"
                     /translation="MEISYVLKDCTFKHNKYSVVEAETWKDKDLDIVVAKGEKFAFQI
                     MLKATEEFNCTIDKSSAISWKGLGNRVRLALNMPNSLENNFSINILGYVQDDTKAFVN
                     DAILRDKDVLVEQYLPQTFWIEGQVPEDFEENNLDIGIDIFKSFGYEDEEKVCTIDVP
                     VKVRNVVLKPLDESKFFLDLWQHPSCLARMYKVELWSDIHFEIVDNYLKELASLGEKV
                     ATVIVSDYPWAGQSCYKVYKNPSNLYEYNMVSVSKGLDGKIKCNFESMDRYIGIADKY
                     KMAKEIDLFGLLGNWCAGEFGNPVEGYKDPIRVRYFDEKDKVFKFINNTNDLKEYIGL
                     VLNHLIECGLWDRVRIIADEPNNPEVVKECIEFINSTVGTHQVKYKSATHDQNFLDRA
                     KDEIDDMSINLKLTIQNYKDIESLKKKINDKGGILTWFVCCFPEKPNSFLSSPFVENR
                     IIGWYTYYFGLDGFLRWDYNLWTEDPWKDSSYKFPIWKAGDMFFVYPGKDLKPVRSVR
                     MENLRFGIQDFELFTMLEKEKVREYIEVELMQELLNKKENAEIKGFGDIELGYSLDNW
                     RYDKVKKYVLELLDRV"
     gene            16206..17186
                     /locus_tag="ACGI59_000878"
     CDS             16206..17186
                     /locus_tag="ACGI59_000878"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - obsolete organic substance
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 5 protein"
                     /protein_id="HFG9738683.1"
                     /translation="MFFLSISFIGFYLVIRDNSDTVKMQRGINIGNALESPKDFPWDV
                     KMSNKFFDDIKDAGFDTVRIPVRFSDYTSDSDNFKIDEEFFKKIDKYVDYALDKDLIV
                     VLDLHHFEEIMKEPRVHKEKFLKIWQQIANRYQKYDKKLVFELLNEPKENLSSQLLNE
                     YIEEAIKIIRKTNPKRTIIVGPYNFYQIDYLNELNIPKDSNIVVSFHYYEPNDFAFQG
                     NIYHKGFEHLSNITWEGTNEQMDYLKKRFDTVENWANKNKVKIFLGEFGITKEAPEVS
                     RRAWIKAVREEAEKRNFSWAYWELASGFGIYNQIEGTWDRDILSALIEKR"
     gene            17191..19497
                     /locus_tag="ACGI59_000879"
     CDS             17191..19497
                     /locus_tag="ACGI59_000879"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFG9738684.1"
                     /translation="MKKNSKKILITLTIITNVIYILWRIFYTVPKEEGKFALICAIIL
                     LFVEIMGMMEMFVHYYGMSNIEYPEKPIISEELYPHVDVFIATYNESVDLVRKTVNGC
                     IHMQYPDKKKVHIYICDDGNREEMRILAEKMGVNYITRTEREGAKAGNLNNAMQHTTS
                     PLIATFDADMIPMHDFLIATVPYFLKNEQAKKDGEKEEYEKVGFVQTPQSFYNPDLFQ
                     FNLHSEGRIPNEQDYFYRDIQLARNRTNSVIYGGSNTVISREALEEVDGFYTYSITED
                     FATGILIQSKGYRCYAIPEVHASGLSPTDLKSLIKQRERWARGCIQTGRRLNILFRRG
                     LGFWQKISYISSITYWYASIKRFVYIMAPILFSVFNVIVVKCTLLQVLVFWLPMYILS
                     SLSLKIFSQNIRNTRWTNIYETIMFQSLMPAVILETFAISKNKFSVTNKSKLEENRMY
                     KFLQGIPYFIYMVLSIIGILKMFVAIFKMSSMTYSVVLFWLIGNLFNLVMATLFISGR
                     QQLRKSERYIAEIDFKLKQNSYVLSSKTIDISENGFAFLLENPEYISPEEEFEVEFRE
                     KSGNEMYIANMKAKIVNVVEVNSKWKYAAYITHIEDSEIDNWMCIVHDRIPTLPMTIS
                     NQLGFFDDLQINVKKRIEKTRTLSRRSPRINMNFQMDIKNTGKLRIVNFNYQYVLLNF
                     ENKSIYPKEIALEINENIVLECDLCEGKIDERGILYKVNNIDSIMQNFFLRDEMMDWI
                     LQNKTILVSKPSEKKEKSIDEFEPMEYI"
     gene            19508..20584
                     /locus_tag="ACGI59_000880"
     CDS             19508..20584
                     /locus_tag="ACGI59_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9738685.1"
                     /translation="MKTLKVFFCSTKYLILVFIFFINTTFISSATSDKILDLSFKKIE
                     TDLNSKITYEDTGVKIETDSSKSDKERYLYIYQNIKENWSMYNNFYIEIQNKNKSSQR
                     INLSIQSKNMLEFRLKEGSEVFLEGKNIIYSDKIKEGCIEVPGEFEGKIYVNFNSLIN
                     EESNVVLDSNMLSNIVSWGITFIPSDEEHNIVIIKKISLLSEEKLRFLNNIKIIGDEE
                     VQIPVLGQSISQYEVLGLKSDSKIKYSLMGKQDNVSISQKGKLTLSNKAKPGQIILQV
                     NVDDKFKIGKKITLTESWSVNKKDKDGVPYTLVYPEQSPTVQDMKKINFMNNIITFVR
                     ILFVSLVIICFGIYLYWKKCSKTK"
     gene            20602..22020
                     /locus_tag="ACGI59_000881"
     CDS             20602..22020
                     /locus_tag="ACGI59_000881"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HFG9738686.1"
                     /translation="MLFSSLIFLFYFLPITLVLYYVFRFNRTIQNMILLAVSLFFYAW
                     GEPKFVVIMIVSIIMNYIFGLLVDRYRESKIKVKVFLVLMCAYNIGVLFIFKYLAFAL
                     RNISELISTELTIPSIVLPIGISFFTFQGMSYVIDVYRRHGEVQKNPFYVGLYIAFFP
                     QLIAGPIVRYESVAEQILNRKETWNKFSIGTCRFITGLGKKVLISNNMAIVADYIYTM
                     NSQGEIAASLAWLGSIAYTLQIFFDFSAYSDMAIGLGLMFGFKFEENFNYPYISKSIS
                     EFWRRWHISLGMWFKSYIYFPLGGSRVANKDIMIRNMFIVWLFTGIWHGAEWTFVIWG
                     ILNFVFLIIERFIMFEKIENHNFIKHIYALLVVNFGWVLFRSPNLKEAYNYFKAMFGG
                     NGAIWSDYTYMFLKEYWVFFIFAFIFSVPIAKKINKFVVEQAKYSMIFNVFYPISAIL
                     LFFISVTYLVTGSYNPFIYFNF"
     gene            22035..23243
                     /locus_tag="ACGI59_000882"
     CDS             22035..23243
                     /locus_tag="ACGI59_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9738687.1"
                     /translation="MNRFFKVRLITAIMFLIILFAFSIFNVCYSGKDTIQEIKNSIAE
                     KSSLKDTISSIDNTVNDKVIYKNTFIETYGYLQKLMGKNEFSKFSVVKDTSGKMHYTY
                     FAKAPNPVGVIADRVRKFSDEMEKQNTKLVYLMTPDKYIKGVTKFPKGIPYSYSNETA
                     DNFLNQLKKYNVDYIDFRENILKSGIPKDDLFFKTDHHWKVETSFWAFGELVEQLNKK
                     YNMNLDENHYYRDKDNYNSIVYPKSFLGSMGRKTGILYGGIDDFTLIYPKFKTNYTYY
                     TDSKSQKFELKGRFEESLILSYPFNADLDLMDGQSDKYFTYLLGNRPLVKIKNIENPD
                     GLKVLFVKDSLIVPTAAFFSSVCSSIDMIDPRYYDGDILEYAKSHDYDFVFISVYPQN
                     LTKEFFPFCE"
     gene            complement(23317..24705)
                     /locus_tag="ACGI59_000883"
     CDS             complement(23317..24705)
                     /locus_tag="ACGI59_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HFG9738688.1"
                     /translation="MESSFLSQFLMITDFKTIFFIILLLGTFFIVRQFEKKKIKFSLR
                     TIYATVLGLILGIIIQAFAGFPNDTTQVIWLQEVSKWYSLFGSGFMDLLKMLVVPLVF
                     LSIIKVIINMKDNNLGTLTFKSLGMLLATTAIAAIVGIIVANIMKLGVGANMPTLVEN
                     QELKEINSLVDTLRGLLPSNPVMAMANGNIVATIIFATFIATSIKRLSKKHFETIKPF
                     IGFIEASYKIIVSVSMTVIKFMPYAVVALLANTITSQGISSIVSVLQFILALYISVAI
                     LFIIHLIFITLNGLNPITYIKNSTEALLLAFTSRSSLGTLPVTIESLVQNHGVDEGVS
                     SFTASLGANMGMNGCAGIYPALMAVTLANMAGVNMDMSFYVMLLIVITISSFGIAGLP
                     GTATMAVSVVISGVGLGSYFPLVGGILAIDPILDMGRTMINVNGAMVSSITVGKSLGK
                     IDKEVFSKKNIS"
     gene            25046..26380
                     /locus_tag="ACGI59_000884"
     CDS             25046..26380
                     /locus_tag="ACGI59_000884"
                     /EC_number="1.8.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HFG9738689.1"
                     /translation="MRVIIIGGVAAGMSAAAKLKRIKPEYEVVVYEKTEIVSFGACGL
                     PYFVGGFFNDADELLARTPEKLREAGIDLNIFREVVEVDSESKKIKVKNIKTDEIYED
                     YYDKLMIATGARSIMPPIKNIKLKNVSTLKSLYDGEYLKKLLSNEDNKRVTIIGAGFI
                     GLEAVEACKKLGKDVDVIQLEDRILPQVFDKEMTDVLEEEINRHNVNLHLDEMVVELC
                     GEDKVEKVITNKGEIDTDVVIIATGVRPNTEFLSNTNIKMLKNGAIVVDEYGRTSVED
                     IYSAGDCATIKNIVSNENVYVPLATGANKLGRIVGENLAGREISYQGSLSSSCIKIMD
                     MEAASTGITERQAKDLGINVKSKFISDYNQTHYYPGRNKIYVKLIYDADTKVILGGQV
                     AGFKDAVQRANVLAAAIFGKMTTNQLGMLDLCYAPPFARTWDVLNVAGNVSK"
     gene            complement(26522..27439)
                     /locus_tag="ACGI59_000885"
     CDS             complement(26522..27439)
                     /locus_tag="ACGI59_000885"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HFG9738690.1"
                     /translation="MIDLTLLGCGGNVPMPNRFLSSVFINYKGRKILIDCGEGTQVSM
                     KLKKCGFKDIDLICITHLHGDHIFGLLGLLSTIGNSGRTSDLTIVGPVGIVDCIRSMR
                     NLVEYVPYTLKIIENPQGNFSLDNKVLRNLEISTISLEHSIECLGYSFNFRRNPKFDI
                     DKATKNEVPKILWNKLQEGQNIVLDSKQYTPDMVLGELRKGVKISLTTDTRPIESIPD
                     FIKDSDLFICEAMYGDDLDISKAVRNKHMTFREAANLAKLGNVKQLLLTHFSPSLDIP
                     SMYLENATNVFENTILGEDRLSLRLNFDE"
     gene            27647..28531
                     /locus_tag="ACGI59_000886"
     CDS             27647..28531
                     /locus_tag="ACGI59_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4846 domain-containing protein"
                     /protein_id="HFG9738691.1"
                     /translation="MKVLKKITILSLITLTLVGCSNGEEKKVVEGSKKTQNSKKDEQP
                     IIVHKDANILKDRYEPPKGFKRVDVEKGSFESFLRNTKLKNYGEKVKYYDGRTKESKG
                     IYDSVFDVDIGNRDLHQCADAIMLMRAEYLYQNKLYDEISFDFVDGFKAKYSKWAQGY
                     RISVKDSGSSWYKATEYSTSYESFRKFMDIVFAYSGTLSLEKELEPVKVEDMKIGDVF
                     IVGGSPGHASIIMDMAENEKTGEKVFMIAQSYMPAQQTQLLINRKNPNLSPWYSLDFE
                     GDLTIPQWTFKRDQLKRF"
     gene            28744..30585
                     /locus_tag="ACGI59_000887"
     CDS             28744..30585
                     /locus_tag="ACGI59_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HFG9738692.1"
                     /translation="MKIRRYLILVIIILITTSQSFISYGLERFEEITIFHTNDIHGRY
                     AEGDDHIQIGNLATLKKETPNSILVDAGDCLHGLPIVNMDRGKSAIELIKCAGYDYIT
                     PGNHDFNYGKDRLFELSKSANLGENTLKFLSSNIFENGKKVFDSNDIKEIDGIKIGFF
                     GLSTQETKSKTGFKNVEGLEFKNPIESATEQVNELKGKGANVIVAICHIGTNSLSKPN
                     SIDIANEVQGIDLIIDGHSHTKFENGKQVGDTLIVSAGQYLENIGEIKLTLDTNDVED
                     IKIENKSARLIGKEEALKYKLDSVVSKKVDKIKEQQEKILDKVIGVTTNTLDGSYENV
                     RTKETNLGNLISDILLDKTKADISLFNGGNIRDTIEKGNITRRDIVDVFPFSNTIVTK
                     ELTGAQIKDVLEHGVKLYPEKSSAFLQVGGISYYFNPKQKEGEKITNIQKEGKPLDLN
                     KKYVVATNDYIASGGDEFPCFSKEPVLKEFGNLESAVIEYIEYKKEISKNVDGRIGIK
                     SKDELISKNHESDLILSENEKNINDNEENLSNNIKSSDSDKNKSLDKTSSRESSNLGI
                     SKREAKEKSPKTGDLGFSNSIIIFIVSSTLICLLNFNQKELKDKKSK"
     gene            30890..31597
                     /locus_tag="ACGI59_000888"
     CDS             30890..31597
                     /locus_tag="ACGI59_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFG9738693.1"
                     /translation="MNNILLLEDDKSLNRGISFKLKKEGYNVFSAFGIEEAKNIFAKE
                     EICLIISDIGLPDGSGFDFCEEIRKKSDVYIIMLTALDEEVDIVTGYDLGADDYITKP
                     FSLMVLISKVNALMKRVNTVKNYTLLVCDDLFFYYIENKLIIRTDDKEEEIILSKTET
                     KLLKYLMENSMQTLTKEQLLESLWDSSGNFVDDNTIAVNIRRLRQKVEKNPSEPKYIK
                     TVRGVGYMWGERSIKKC"
     gene            31591..32901
                     /locus_tag="ACGI59_000889"
     CDS             31591..32901
                     /locus_tag="ACGI59_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFG9738694.1"
                     /translation="MLKNMNLKDGLSVKDDYIMRRVIVYSVLSVLIVTLVFILFEYSR
                     LHKLYEHQIKNTQNVAGILIEKYPNDEIDIVNSIYNSDYSKVSTGEKAFKKFGYGLEN
                     KMSNDKNFSLYLKSFFKESFFIFLMMFIVVIGIIYYFIRYINKRLSKIYFIVDKMSQE
                     NYLKDTTESEETGYLVEYNEYFKEGIFSKINNCLYELNRSLKIKFIKLEKEKESVKSL
                     VTDISHQLKTPLASLKLYNTLLIEEELEEDEANEFLLTNKASINKLENLINSLVNISR
                     LEISMINIKKEDNDIKNTILNAVKSVTPKAKSKSITLNLNEFNSRIIPHDKKWTEESI
                     FNVLDNAVKYTQVNGEINISVEETANFFKIIIEDNGIGIEKSEFNNIFKRFYRGSADE
                     IESVEGSGVGLYLSRKILEEQGGNIIVSSKVGIGSKFSLFLTTM"
     gene            33018..33701
                     /locus_tag="ACGI59_000890"
     CDS             33018..33701
                     /locus_tag="ACGI59_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9738695.1"
                     /translation="MKILYTENLSKHYGKGESLVRALDNVDLEINEGEFVAIIGKSGS
                     GKSTLLHMIGGLDIPTSGKVYIDNKNIFTLKEEELAVFRRRKIGFIFQSYNLIPSLNV
                     WENVVLPIGLDGREVDEPFIKELLKSLGLENKHDVLPNTLSGGQQQRVAIARALATRP
                     AIILADEPTGNLDSKTSDEVMSILKSMSKKYSQTLVMITHDDSIAQMADRVIFIEDGR
                     VSKVGDKND"
     gene            33694..36081
                     /locus_tag="ACGI59_000891"
     CDS             33694..36081
                     /locus_tag="ACGI59_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFG9738696.1"
                     /translation="MINTTKIAKSNLKQNKSKSILIIITIILSTLMLSSIGIYIVDAG
                     AYQKENTIKYSGNYQGILANVDEKQADILSNHADVELTGKMNGVGVEKLEDDSNISLA
                     YMNEDALKLNSFEFIKGKLPSKENEIVLDSGALKALGYKNKDLGEKIKISYNDYKSDK
                     KIEKEFIISGILKTSEISEAGKYYYAIISESYMRNTRDMSQEDFNIYVKLNDKSNLSI
                     EQAKSKLNKIANDVGLDTINVAVNENYINALKPDMETIMGGVFVGLVIVLSSILVIYN
                     IFYISIVTKVQEFGKLRAIGATKKQVKNIVFKEGFILAGIAIPIGIILGYVLANIIIK
                     SFMDIDVKSSRLPVILSVVVISFISVVLSLLKPMKVASKVSIVDAVRYTGNKISNKSK
                     RKGYKNINLKRLSHANLERNKKRTYMTLASLILSGTIFITVSTALESFDAEKMAREHF
                     PYDIEVRLSGYEMNSDKNPKDNLNILQMDNPLSKDFFNQIKNMEGIKRIESARSVKIG
                     MEDYDVEFKYDLLQSINENDVKSLNKNIIDGKINLERLQTGDEIVITHVDTAKEMGVK
                     AGDKIRLTLYDGDKKIKKEFKVQAIAMGVPSFGIGKDFIDRTLKYDSTSSLGIYTEEG
                     KYQEIKDSIKKIAKNNGFLETDFIDSRIESNKATISFIKIMGYTLTGIIGVIGFMNLV
                     NTMITSIVTRKKELGMLQAIGLTNKQLVKMLNSEAMYYTSGMMIGSILFGGILGYIAV
                     IFLKKTGLSYATYSLPIVPILLMIVCILIAQFITTYLIGRSFNKESLIDRVRYSE"
     gene            36361..37503
                     /locus_tag="ACGI59_000892"
     CDS             36361..37503
                     /locus_tag="ACGI59_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HFG9738697.1"
                     /translation="MNKKINYAPGPTETRENVRLARAEKTTNPDIDIDFVEFYKKTCE
                     KFGNIIGTKNDVYILSGEGILGLEAACASLTEKGDRVLVIDNGIYGEGFKDFVTMYGG
                     EYVLFSSEYTKSIDIDELKAFLDEDSNFKYATVVHCDTPTGVLNDVSKICPLLKEYGI
                     LTVVDSVAGMVGERLSVDESKIDIILGGSQKAISAPAGLTIVGISQDAKNCIKNRKTD
                     VIGFYCNLNIWEGYYEKKYFPYTMPISDIMGLDKALDNILEEGIENVLNRHEKIAYSV
                     RKSIEEYGLELFLEEGYSNTVTAIKIPESIGALELTDYMLKNYNTLVATSLNQYMDTI
                     LRIGHMGENANLNKIEHVLNVLDKSLSALGFKGNGNLLNLFNKYYF"
     gene            37522..38046
                     /locus_tag="ACGI59_000893"
     CDS             37522..38046
                     /locus_tag="ACGI59_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFG9738698.1"
                     /translation="MEVTAKQKFTTKDMVETSLLIALVFIATKFINIRLPISINGGLV
                     HLGTAMLFISAIVFGSKKGALSGAIGMSLFDLISGWTLWAPFTFIVRGVMGYLLGKIA
                     WANGNNGNNFLINVIGICVSSIWMLFGYYVTEVILYGNFIVPLTSIPGNLMQVLIGLI
                     IALPISKVLKKCIK"
     gene            38146..38964
                     /locus_tag="ACGI59_000894"
     CDS             38146..38964
                     /locus_tag="ACGI59_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6597 domain-containing transcriptional
                     factor"
                     /protein_id="HFG9738699.1"
                     /translation="MLSVTRYSIKNVRLKPWVKFIWYFETKSNILLNNKLLPTDSIDI
                     ILNLSDVMEYKIENQDYIASNIHFNGIRDKHGFIIQHGNIRVIGISFYPFGLYPFLKI
                     PISEFNRQIVDLEAVSKLFAKKLEESLNPTKAIEKVVLCLEEVLLSILEEDLISNKYV
                     KLLNSFIYVNRYSNIKAFCDDTNINIKTLERICLKYTGYTPKILKRIYRFKMASNQLI
                     YNYKDDELFDFIYENEYYDQAHFIKEFKKFSGTSPIKFSGENKTIKENIKYSYL"
     gene            39042..39353
                     /locus_tag="ACGI59_000895"
     CDS             39042..39353
                     /locus_tag="ACGI59_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HFG9738700.1"
                     /translation="MKIITEIVEFNVEENLSKEEFIEIVNDLELKFHSIQSGFIDTEL
                     LYDEKSQKWIMIQHWESIEDLKSVSSKMFKDSSTEKFRKALKNQTVKMNIIPQIKSWK
                     L"
     gene            complement(39530..40213)
                     /locus_tag="ACGI59_000896"
     CDS             complement(39530..40213)
                     /locus_tag="ACGI59_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9738701.1"
                     /translation="MQKEVAMNKLIKDCVNFFNDCGFSYSICGGYALELFANMHIRPH
                     SDIDIWISDTDKEKSIQYMLNQGWDIYEPLGNYLLRPIIDLNKQHIEQICVFAIKPDC
                     SFIELKKVEDDNYKMNIQCDEQLQFDFIELIYNPQKDGKFLFSQNQRIIRDMDKARLI
                     TSDNIPYMAPEVVLFCKSIYIDRLGYQKDFDVTVPLLSQEQRDWLVNALITAYPDGHA
                     WIDQLANQY"
     gene            complement(40306..40956)
                     /locus_tag="ACGI59_000897"
     CDS             complement(40306..40956)
                     /locus_tag="ACGI59_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFG9738702.1"
                     /translation="MSFLGRKKEPDNTIQKILDISHKLFLEKGYEQTTIQDIVDNLDG
                     LSRGAIYHHFKSKDEIIKAITSRLYNDGFYGYEVKNDEGKTALEKLQYRLLHTYEQHN
                     KQDIFLMSSSLLRNPKYLATQFDEIINDIVPWIKALIEEGNQDNSMEVKNPEIAAQMF
                     GFLLNTWCIPTVYPGNPQQMIERFTLFGEQAAFMGISFMNDKIMCEYQKFVNKFSK"
     gene            complement(41121..43124)
                     /locus_tag="ACGI59_000898"
     CDS             complement(41121..43124)
                     /locus_tag="ACGI59_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFG9738703.1"
                     /translation="MKSQKRFKILDIDVYLQPFESDIKKRMKHYEDMKSKTLKNYKDF
                     SSFANAHLYYGFHQTNDGFFYREWAPNADSLSLIGDFNNWNRKSHPLRKIGSGNWEIF
                     IPGQNSLPHKSEVKVQVTANGKTFDRIPLYIKRVIQKDFGGFNGQIWQPKTPFIWTDN
                     DFDLKNITSPLIYECHIGMSTESESIGTYNEFTEKILPKIKKAGYNTIQLMAIMEHPY
                     YASFGYQVSNFYAISSRFGTPEDLKNLINTAHSMGIAVLLDLVHSHAVKNTLEGINEF
                     DGSEHQFFHSGSKGNHPAWGTKLFNYGKPEVIHFLLSNIKFWLNEYHFDGFRFDGVTS
                     MLYHNHGLGVSFDSYEKYFSMNTDIEAITYLQFANELMKEIKPNSISIAEDMSGMPGM
                     CIPIKDGGIGFDYRLAMGVPDFWIKTISNLSDEDWDLGKMWYELTTRRPGEKNIGYCE
                     SHDQALVGDKTIIFWLADKEMYWNMEINSNNHVINRAISLIKLIKLITFSLAGEGYLN
                     FMGNEFGHPEWIDFPREGNNWSYKYARRQWSLSEDNNLKYKQLLNFDKSMLELTKYSD
                     IFIQNSHELIHMDNDKKILVYSKGKYLFIFNFHPNLIQSVDISKFQNTNYKTILNTDM
                     VEFGGNTKKENLRDYDVYFSSTPPSSKMPIASRTAIVLLDNGI"
     gene            complement(43277..43915)
                     /gene="recX"
                     /locus_tag="ACGI59_000899"
     CDS             complement(43277..43915)
                     /gene="recX"
                     /locus_tag="ACGI59_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFG9738704.1"
                     /translation="MGIITKIEQQKRNDDRVNIYVDDKFFIAIFKELVYTFNLKKGNE
                     INEEELKPILDDEMYIKAKNKALNILSHADQSEKKLKEKLSSEFDENIIERVIDFLKS
                     YNLVNDSVLAQKIVNTNVNLNKCGKNRIKQNLYNKGINRSTINEAVSELDKDTEFENA
                     MYLAKKRYERVKKEDKKKIYQKISQHLSYKGFDYDIIKRVLNKLLNFDEYDI"
BASE COUNT        17095 a         4802 c         7550 g        14473 t
ORIGIN      
        1 aaaacttttc taggtttaaa ttataccata tttatagtat gatatgatta taattttata
       61 taataaataa gtaaaaatag taaaaaataa attaagaaat gtttttaata aaaataagct
      121 taagggggtc aaccatgagt gcaaaggtaa cgaatcaata tggaaatata gaaatagata
      181 atcaagtaat agctcaagtc gtatatagag ctgcaatgga aagttatggc ttagtaggtt
      241 tagcatataa gtctaaagga atagtagagc tattaaaagg cgagaacgca actaaaggtg
      301 taagagttga ggaagtagaa gaagatgcca tagcaatcga actttatgtt ataatacaat
      361 atggtacaaa tatatctact gttgccaata atataataga tagagtaaaa tatgtagttg
      421 aaaaaatgac tggcgtaaga gttactagaa ttgatattaa tgtacaagga ataagagtta
      481 agtaatttca taggaggaaa caaatgattc aatatataga tggtaaaagg ttaagggaga
      541 tgtttatttc aggtgcaaac aatctccaaa acaataaaga gttagttgac aagttaaatg
      601 tattccctgt accagatgga gatacaggaa ctaatatgtc attaacaata tcttatgcct
      661 taaaagaatt agcaaaagtt gaaaatgata atatatcaga tatcggaaaa gcattatcta
      721 aaggttcttt aatgggagct agaggaaact caggagttat actttcacaa ataatcagag
      781 gtatagctaa atctatagaa ggaaaaagta aattatcaac agaagattta gcaaaagctt
      841 ttaaaaatgg ttcagacaca gcatataaag ctgtaataaa accaattgaa ggaactatac
      901 ttactgtagt tagagaaagt ggagagtttg caataaaaac tgcgaaaaaa gagaaagatg
      961 tagtaaagtt tttaagtatg cttgtgaagg aatcaaattc ttcattagaa agaactccag
     1021 accttctaaa gaatctaaaa gaagctggtg tggttgactc tggtggaaag ggtcttgtac
     1081 ttatatatga aggaatgtta gcttctataa aaggcgataa catagaaata aaaaatgttg
     1141 acctagatac taatatttca acaagtatgg attttgcaaa gagtactact agcacagatg
     1201 atataaaata ttgctattgt acagaattta ttttagaaag ttctaaggta gaagatacta
     1261 aaataagaga cataatgatg gcttatggtg atagcctagc agtagttgga gatgatggag
     1321 tcataaaggt acatgtacac actaatgacc caggaaatgt acttcaagaa gctctaaagt
     1381 acggtcaatt attaactata aaaatagaaa atatgaagtt acaacatgaa aatacattac
     1441 tggatgtaga agaaaagaaa gaaaatgata gtgaaccttt agaagaagaa aaagagtttg
     1501 ggtttatagc tacttctatg ggtgaaggtt tggcaaatat atttaaagat ttcggagttg
     1561 accatataat agaaggtggt caaactatga atccaagtac tgaagatttt atgaatgcta
     1621 taaagcatat aaatgctaaa aatatattta ttttcccaaa taacagcaat ataataatgg
     1681 ctgctaatca ggcaaaagaa ttgagtgata aaaatataat agttatacct acaaaaaata
     1741 cgcctcaagg atttgctgca ttagtaactt ttaatggaga attaagcgaa gatgaaaata
     1801 aagaagctat gatgaatgct ttaaactcag ttaaatcagg tcaagtaact tttgctgtta
     1861 gggatacagt gatgaatgaa attgatgtta aagaaggtaa tataattgga attgcagaag
     1921 gaaatttatt atcagcagga gattatgtgg atgaagtaac atcaaacctt attgaaaagt
     1981 tggttgatga agatactgct ataataacat tattctttgg tgaagatgtt acagaatctc
     2041 aagcaaatga actacgtact tctttagaag aaaagtttga agatgtagat gtagagttat
     2101 attatggagg acaacctctt tattattact taatttcagt tgaataatga ttataagaac
     2161 ctgtatgacc aagtagcttt tataaaattg gttgtacagg atttttgtgt agtaggtgat
     2221 atatttgttg gatttatata aagatgttca atatgttaag ggaataggac ctaaaaaagc
     2281 agataaatta aataaactgg gtatatttac actaaaagac ctattgtatt attttccaag
     2341 acaatttgaa gatagaaata atctaaagaa aatagcacaa ttagaagatg gtgagaaagt
     2401 tacgataaaa gcagtaatat caagtataaa tacatttagc ccaaaagaag gtatgacttt
     2461 aacaaagatt gatgtaaaag atgagacagg ctcggctaaa ttagtgtttt ttaataagag
     2521 ttatataaaa aatacttttc gtccaggaga ttcaatatta gtttttggga aggttaagaa
     2581 aaaatttaat aacttagaac ttacatcatg tgaattagaa tatcttacta attcacctaa
     2641 gaatacatgt agatttatgc ctgtgtacca gcttacatac ggtgttacaa ataaagaaat
     2701 tatgagtata attagaacag tacttgaaga taaagaatta attatacaag aatatatgcc
     2761 acaaagaatt atagaaaaat ataggctatg tagtatagat tttgcagtta gaaacataca
     2821 ttcaccaagt agtaaggaat cattaaaaat agctttatat agaattgtat ttgaagaatt
     2881 acttatacta caactagggt tgtttgtatt taaaagtggc agaaataaag aagatggaat
     2941 aaagtttgaa acaagtaaag acttaaaaaa aataataagt gctttaccat ttaaactgac
     3001 taaagctcaa aacagggcat tagatgaaat tatacaagat atgaatttag agaaaataat
     3061 gaatagattg gtacaaggtg atgtagggag tggtaaaaca gtagtagctc tcttagcact
     3121 tgcaaattgt gttttaaatg gttatcaggg cgctttgatg gcacctacag agatattagc
     3181 agggcaacat tatatttcac ttactgagtc tctaaaagat tttggaataa atgtaggtct
     3241 attaataggt agtcttacaa aaaaacaaaa ggatacagta ttggaacaaa taaagaataa
     3301 tgagattgat atattaatag gtacacatgc actaattgaa gataaggttg aatttaataa
     3361 tattggttta gttataacag atgaacaaca tagatttggt gttatgcaaa ggagcaaact
     3421 ttcattgaaa ggagcaaatc ctgatatatt agtaatgaca gcaacaccaa taccaagaac
     3481 tttagcactt atactctatg gagatttaga tatttctatt atagatgaac ttcctccagg
     3541 aagacagcca atagaaacaa ttgctattga aaaaagtaag cgagatagag catataataa
     3601 tcttgtaaga agagaggttg aatcaggaag acaagtgtac attgtatgtc ccttggtaga
     3661 agaaagtgaa gccatagaag ccaaatcagc agttgaactt gttgaggagt taagagcgga
     3721 atattttcac gacttaaggc ttgggttgct tcatggtaaa atgaagtcaa gtgaaaaaga
     3781 tgaagtaatg agacttttta aagataaaga aatagatatt ttagtttcaa caacagtaat
     3841 agaagttggt gtcaatgtgc caaatgcaac attaatgata atagaaaatg cagaaagatt
     3901 tggacttgca caattacatc agcttagagg gcgagttgga agaggtagtc ataaatcgta
     3961 ttgtgtacta atttatgatt ctaaaacaga tgtatgtaga caaagaatgg ctataatgga
     4021 agagaccaat gatggtttta agatttcaga gaaggattta gaaataagag gacctgggga
     4081 attttttggg acaaggcagc atggtcttcc agaacttaag gtagcaaact tatttaagca
     4141 tataaaaata ctgaagttag cacagcaaga agctcgatat atattgggtg aagataataa
     4201 tctacaatta aaagaaaaca tggcactcaa aaaagaaatt atagataaat ttaaagatac
     4261 attgaaagaa atttcattaa attaatttta aattgtgtta aaatattact aatgtaataa
     4321 tgtgctgaag ggagattgtc atattgagag taatttcagg aaaagcgaga ggattaaaac
     4381 taaatactcc aaaaaatgaa gatgtaaggc caactacaga tagagtaaaa gagtctctat
     4441 ttaatatgat aaattcatat ataatggaaa gtgaagttct ggatttattt gctgggactg
     4501 gttctttagg aatagagtgc ttatctaggg gtgccaagtc atgtactttt gtagatatta
     4561 gtaaggaaag catagatatt gttaagtcca atataaaaaa ggcaagagtt gaaagtgaaa
     4621 gtattatttt aaaccttgat tttaagactg caatagataa acttaaatta caaaatagta
     4681 aatttgacat aatttttatg gaccctccgt attataaaaa tatgtttata gaagctattg
     4741 aaaaaattga taattctaat ctattgaacg aagatggaat aatagttgta gaacatgata
     4801 cgaatgattt atttccagat aagatatgta aattggagaa aactagagat aagaaatatg
     4861 gaaatactac attaactttt tataagatgg aggcataata tggaaaataa acctagaaaa
     4921 gctatatttg caggaagttt tgacccaatt actaatggac atttagatat tatttgcagg
     4981 gcttcgaagt tatttgacga attacaaata ggagtattaa ataatcctaa taaaaagggt
     5041 ctatttagct ttgatgaaag agtaaaactt atagaaaaaa gtacaagcca tttaaataat
     5101 ataaaagttg tgacttttga tggtttatta ataaattact gtcaggaaaa tgggattgga
     5161 gcattagtta gaggtgtgag aagtggtgct gatgttgatt atgaacttca gatggcacat
     5221 atgaatagag agcttaatcc agatatagag actataattt taccaagttg tacaaaatat
     5281 tcatttataa gttcatcttt aataaaagag gttttgctct ttgatgctga tataaaaaat
     5341 ttggttccaa agattgtttt ggaagaatta aagaaaaaaa ctagtggggg taattgatat
     5401 atgaagatag atttagaaat gattgaacta tttgagcagt tagaggagat atttagaagt
     5461 gcttctacaa tccctttctc acataaatgc acagtggata aagatgaggt tttagcaata
     5521 attaatgata taagaacttt aataccagaa gaagtaacac aggcagtatg gattaataaa
     5581 gaaagaaata aaataataag tcaagctaag caagatgcta caaatatagt tgaacaagcg
     5641 aaaaaagaag ctgagagaat acaacaagaa tatcaagata atatagagga attaaagaaa
     5701 aattctgaag atgtagtaaa ggcatatgta gaatccagtg aacctgttgt tcaagcagac
     5761 caaagagcaa aagatatagt tgatagagct gagagaatag ccaatgagat aagaattggg
     5821 tcaattgaat atgcagagga tgtgctttct agtgttgagt ataatttaag agaaatatta
     5881 gaagaagtaa aaagagataa agctgaactt agacctaaaa aataaatttt aaataaataa
     5941 aaatggagct atcacacaaa aaattccaat ttatgtgtga tagctctctt ttaatatagc
     6001 aattttttgt tatataattg tgctttttag attacttata tcttattttg atattataat
     6061 gtagtttaat cttttatttg ttgaaatcaa cttatttatt tgtataaata atataggtta
     6121 ttttcaaatt tttttaataa ttttaccact gtcttctttt agattagagt tagactgtgc
     6181 aaaattataa aataatacta aatcatgtgc aattatttgg tatggcacta catgtagaaa
     6241 atatagataa taaaattaag gaggtagtat gaaggaaagg gctaaaaaaa tattagattt
     6301 tattattaaa aataacaatg taacatcaca agaactacaa gaaaagttta atgtttcaaa
     6361 gagaactatt tattacgata ttctagctat aaatgaacaa ctaggaaaaa gtggtaatat
     6421 aaaaaatgta aaacacaaat ttatatttga aggtaattta tgtgatgcaa ggaaaataat
     6481 ttcaactgaa gaagataaat ttttagatag tgattacaga aaaactttta tattaaacaa
     6541 gatattactt ggcgaaaaaa tatcaataga aaaactaaca aatgaaatgt tactttcaaa
     6601 aaacacagta gtacaaacaa taactgatgt aaaaaaatac cttcaaacaa tggggttaag
     6661 attagagtat aagggaaagt acaaaattat aggtgatgaa tacgttatta gagagctgtt
     6721 tctgataatt gttcaagaaa atatattgga aattaattct ataagtgagg aagtaagtag
     6781 tttcgataca aaaggacata taaaacttac tgattattct ttattaaatc taactaagtt
     6841 tgtggaattt ctaaataaaa ggataagaga tgggaaaaca ttatactcat ataaatatct
     6901 aaatgaagct aaaaaaatta gttactttag taattgtaaa gaacttttat gtgaagaagc
     6961 taatgaaaat gaacaagcct atatatgcac ttacataagt tcacttccca gcttaaatag
     7021 tgaagttaag gaagatgttg ttgaagaata tgtagataaa cttatagata agtttgaagt
     7081 aaatacagca ataaaattag aaagtaagca tgagtttaag aaaaatattt taaggcacct
     7141 ccattcttct tacaacagaa tacgatttaa gtttcctatt agaaatccaa tgttagatga
     7201 gactaagtat aaacatgagt cactttataa aattataaaa tcaattatag aaaatgaaga
     7261 agagtttcct gtatttgagg ggataagaga agaagaaata ggatttatag cagcgtattt
     7321 tggaggatat ttaagagggt ctagggataa tggtctaaga agaaataaag tcttactagt
     7381 ttgtcctaat ggtcttatgg tatcaaagag tttggaaata caactatata aatacatacc
     7441 aaccatagaa atagtaggta tagtatccat taagcaacta aaagaagtaa atgtatacta
     7501 cgattacata attacaacta tagatataca aaatgtaaac aacgtaattg ttgtaaatcc
     7561 tcttttaaca tcatcagatg ttcaattact tatgaacaaa ctaataagtg taaaagaaaa
     7621 cgagaagtat ttcaatcttg aattaataat acaagctata agaaaaaatg gagttatcaa
     7681 taatgaagaa gcactaaaag ctgatttatt aaacataata cacaagatag atgaaggaga
     7741 gatgtatcaa ccaatgttaa aggaattaat taatgcagaa agggtcaata taataaaaag
     7801 tgttagagat tggaaagaag caataaaaat agcctcaaag ccattgttag aagataactc
     7861 tatagaagag ttatatatag aaaatatgat aaagtcagtt gagaaatatg gaccatatat
     7921 tgttttagct gatagatttg ctctaccaca tgcaagttct aaagaaggag taaacaagct
     7981 tgctatgtct ttactaatag tagaagatga agttgattta ttaggaaaac cagtaaatat
     8041 atttatggta ttagctgctg tagataacac tactcacatt agagcattag catcactatc
     8101 agagatgatg tacgaggaag aaaatgtaaa actaataatt aatggtgata aaagttcaat
     8161 actagaactt ataaataagc aaaattagga ggaatgaaaa atgaagatat tagcagtatg
     8221 tggttttggt gtaggttcat caatggtatt aaaaatgaca atacaaaagg ctctaaagca
     8281 gttaggtata gaatgtgaag tagaaaatac agatataaat tcagctagag gtgctgattg
     8341 tgactgtata ttcacaagtc aagaattgtt aaatgaatta aaaggaactt gtaatgtaga
     8401 tgtatatcca gttaaaaagt acatggattt agcagaagtc acagaagttg ttcaaacttt
     8461 tttaagtgat aaaaatgata agaaatagta taagttttaa aaagtaaatt taagttaatc
     8521 agaataaaat aaaaacttac taaattcaaa aatagtaaga aagagaggag ggatttagtg
     8581 gtagaaaact taaacagttg acctatcact aaaaaatata atgcttgatt ttatttcaaa
     8641 taatatttta cgtaatccat ctatgttaat tggattaata gcagcatttg gtcttgtact
     8701 acagaagaag aaagcatctg atgttataaa ggggtcacta cttgcagctt tcggaattat
     8761 aattatggag actggaacag gtatgttagt tagttcaata gcaccaatta atggagcttt
     8821 ccaaagtata agtggtggtg cagtaacaaa aggtttaagt gatgttactt ttactgcaag
     8881 ttatggtgga attataggtt tagcaatgtt tgcgggttta gctattcacc taatgatagc
     8941 aagatttact cctattaaga caatcttttt aacaggtcac atgctttatt ggttcccatt
     9001 catatttgta gcttcaggag ttgaagctgg attaaatggt ggagtattaa tagcttttgc
     9061 agcagtatta tcagcactct attggtcaat aatgccttgg ttattaagaa aatatgtgtt
     9121 tgatgttata ggagatgatt cttttacatt aggtcatcca tctggttgtt tagcacttat
     9181 atcaggattt gttgctaaat tagtaggaaa taaagaaaaa tctacagaag atataaacat
     9241 acctcaaagt ttatcatttt ttagagaaat atctataagt ggtggaatgg cagtgttttt
     9301 aatgtttata gttgttggga tagtaatacc aggtgcgttt aaagatagta atgagtttat
     9361 tggaacgtat gcaataaagc aaggtctttc ttttggagca ggtcttataa taatgcttca
     9421 tggggtaaga atgctaataa atcaaataat gccagcattt caaggtatat ctgaaaaagt
     9481 tgtgccaaat gcactacctg cttttgactg tccaatatta tttaattata aaccaaatgc
     9541 tgtattaatg ggattcttag ttgctatgct aacatctaca gctatgattt taatatgtaa
     9601 ctactttaat gtatttaatt tgctacttat accacttgtt ataacttcat tctttgagtg
     9661 tggagcagca gcagtaatag gagaatcaca aggtggtata cgtggagcta taataggtac
     9721 agctatagct gcaatattta tggtagtttt agtaggaata tctgctgtaa tttactcagg
     9781 aactattcaa aattggatac taatatttgg aggtaatgac ctttcattat ttggtataat
     9841 cagtaggttt ataggttcac tatttggagg aatattatag atatgaaatt taaatacata
     9901 gatgagataa gaagattaat tgatataata gaaaacgaag aaaaagtcaa tatggaaaaa
     9961 gcagtacaaa tctttgttga agcaatagaa agtaaaaatg ctatattttc atttggagct
    10021 tcacatgcag gaatattaac agaagaatta ttctatagag caggtggctg tgttcttata
    10081 aatccagtat ttgcaaaatc tctaatgtta gatacagcgc ctataactca cacaagtaag
    10141 atggaaagat tagaaggata tggtacagct ctagctgata aaactccaat taaaaaagga
    10201 gatgtagtta tagttcattc agtatctggt agaaatccag taagtataga gtttgctatg
    10261 aaagctaagg aaaataaggc aatagtaata tgtataacta atttaagtta ctcaaaaagt
    10321 gtatcttcaa gacattcttc aggaaaaaaa ctttatgaag tatgcgattt agtgatagat
    10381 aatcatggtc aaaagggaga tgcctgtgta gagatagatg gattaaacca aaaagttagt
    10441 ccaacttcta ctgtaatagc aactactgtg atgaactcta ttatagctca agctactcag
    10501 gaattagtta ataaagggct aaagaatcct ccgattttct atagtgcaaa tattgatggt
    10561 ggagatgagt taaataagaa gatttttgat gaatataaaa gtgtaattca ttatgaatat
    10621 taaggttaaa aaaatatgtt aatgaacttt gaagtttatc aacaaagaat attaataata
    10681 tataataaat gctatcattt aaaagctgta ggtaaaaatc tacagctttt tattattgta
    10741 gattaaaaag atgcgtaatt aaattttaaa ttggagaaaa taaattggaa ggtatcaatt
    10801 ataaatatta gaaacgttgt catagaaaaa aactatatta aaaaaaagta catagaaaaa
    10861 actgattaga caataaatac atgctttgat aatattttaa atggagtgaa aatgtgtaga
    10921 attataatga aaaaagaaaa taaaaatgaa aaaagaaaat aaaatggagg gaataaaagt
    10981 gggtatgtta aaaaaattat gttcactagc tatggtagca attatgacta caacacttat
    11041 aacagggtgt tcaagtggaa atggaaaaga ttcaaaaaag gatggcgaaa aagaaaaact
    11101 agttctttgg atgccaccag tagaaaataa tatgaaggaa gtttgggacc ctattttaga
    11161 taaatttgag gaaaaaaata actgtgaagt agatttacag ataattcctt gggataatta
    11221 ttctgaaaaa tttgcaacag ctataagtgc tggcgaaggt ccagatgtag gatatatgta
    11281 tgcagaaatg tatccacagt ttatagaaag tggagcagta gaagatttga ctaactatgc
    11341 aactaaagag gataaagagc aatctattta tataaaaaca tctgaaatga tgggtggaat
    11401 gtatggtatg ccattccaag cagcaaatcc aggggtatta tactataaca aggatatttt
    11461 agagaaacta ggagaaaaac ctcctaaaac atgggaagat tttaaaagaa tttgtaaaaa
    11521 agcaacgcaa gatactgatg gtgatggaaa gatagaccaa tggggattag ctcagggatg
    11581 gggagctaaa acttttggta acttaaactg gaactggtat ccttatttat ggcaagctgg
    11641 tggagatata tttaatgatg atttaaaatc ggttaaattt aatgataaga gtggtttaga
    11701 agcagctaag ttcttaaaag aattacaagc atatgtgcct gaagattctt tatctaaaga
    11761 tagtaatgaa atgatagaaa gtgtatttgg accagggaaa gcagcattta caataatgtt
    11821 atcttctgct gcaacaagtg tatttgatga atcattcccg gatttaaatt ggggatttgt
    11881 aactggacta gaagataaga aagcagcaac ttttggagca gtagaccatt tatctttaat
    11941 ctcatctgcg aaaaataaag aacttgcata caagttaata caacatatgt taagtgttga
    12001 atcaatgaca gaatttcata aagcaatacc aagagcacca ataacaaaag gtgaaccata
    12061 tcaaggtgat gaaagattca aagaaatggt ggaaaatgac aaaaatgtat atagaccatt
    12121 agtagtagga cctcatggag ttgaaatata tgaatattta tggaaagaac ttcaaactat
    12181 gatttcagga gataaaactc caaaacaagc gttagatgat gctgcaaaat attcaaatga
    12241 tttattagca caataaaagt ttaaagctgt aatatgttgt aaagtacttt acagattaaa
    12301 attagcattg tatttataaa tacaatgcta atatttatat tggagaggat aaaatggaaa
    12361 acttagttag agagtttgaa gaaaattcaa aaagaaaaaa aacaaaatta aatattaaac
    12421 catatatgta tatattgcca ttgggaataa ttttagtggc gttttatgtg ataccaataa
    12481 taatgtcaat atactttagt tttacaaagt ataatattat tagtccagct acttttatag
    12541 gtttagaaaa ttataaaaaa ttatttacag atgaaacatt aaaagtatct attataaata
    12601 ctattaaatt tactgttgtt gtagtacctt gccaaactat attatcattg attttggctg
    12661 tttggattac aggaaaagga aatagtaaaa tagcttcatt tgctaaaggt gctatattta
    12721 taccagtatt atcatctatg gttttgatag gtatggtatg gagggcttta ttgaatggag
    12781 aaggttctat tatatatcag gtattaggtg cctttggaat agaatcatct aaacttttag
    12841 gagatagtaa gacagcatta cctacactta tgtttatatc tatgtggaaa aatatagggt
    12901 atttcatggt aatttatatt tcagctataa tgaatttacc aaaacactgc tatgaagtag
    12961 cgaaggttga tggggctaca aaacttcaag aatttataaa aataactgta cctttattga
    13021 aaccaactac aataatggtt gtatttttag ggtcgatatg gtcattacaa gtatttgact
    13081 taatttatac tgtgacaggt ggaggtccag gtatatctac tatgagtata gttatgcatg
    13141 catttaactt aaactttaaa aactttaatt ctggatatgc aatgactgta gcaaacgtat
    13201 tatttttgtt aatagcagta gtttcgattt tacaaaataa gcttgtaaaa cgagacaatt
    13261 cagattttta gaaaggtagg tataatatga aaacagcaag aagattatta agtgatggtt
    13321 tacttttatt tatagcgtgt gcaagtttgg taccttttat atacatgttg ataatatcat
    13381 tgaagataac atataactca tatagtttgg atatatcttt ttcaacagta acccttcaaa
    13441 actatataga tatttttact aaaaaaggat ttgcacaata tttttttaat acagcaatag
    13501 tatctttttc aggtgtatta ttaaatttag tatttagtac attagcagga tattcttttg
    13561 cgaagatgga ttttaaaggt agtgataagt tatttttatt tatgataatg actttaatta
    13621 taccttccca agttacaatg ataccactat atataataat gaaacactta ggatggataa
    13681 atagttatct tgcacttatt atgccaatac caactgcatt tggcgtattt ataatgagac
    13741 aagctatttt aggagtacca aaagagttat tagaatcagc aaagatagat ggttgttcgg
    13801 attttagaat tttaatacaa atagtgttgc ctttaattaa accagcatta attacactag
    13861 caatatttac ttttatggga gcatggaacg agtttatgtg gcctcttata gctacaacta
    13921 aagatgcaat gagaacatta acagttggat tatctactct taagacattc cagataacta
    13981 attatggaca gatgatggca ggggctacaa taacattttt acctccattt attttctatt
    14041 tgattttaca gagtaaattt gttgaaggtg tgtctttgtc tggtataaaa ggttaaatag
    14101 tctaatttaa taatatataa agaaacttat ataaatatgg aggaatttaa actatggaaa
    14161 tttcatatgt actgaaagat tgcacattca aacacaataa atactcagtt gtagaagctg
    14221 aaacttggaa agataaggat ttggatattg tagtagctaa aggtgaaaaa tttgcatttc
    14281 aaattatgtt aaaagcaaca gaagaattta attgtacaat tgataaaagt agtgctatat
    14341 catggaaagg cctgggaaat agagtaaggt tagcattaaa tatgcctaat agtttagaaa
    14401 ataattttag tataaatata ttaggatatg ttcaagatga tacaaaggca tttgtgaatg
    14461 atgctatttt aagggataaa gatgtgttag tagagcaata tttgcctcaa acattttgga
    14521 ttgaaggtca agtaccagaa gattttgaag aaaataacct tgatataggt attgatatat
    14581 ttaaaagctt tggatatgaa gatgaagaaa aagtttgtac aattgatgtt cctgtaaaag
    14641 taagaaatgt tgtgctaaag cctttagatg aaagcaagtt ctttttagat ttatggcaac
    14701 atccaagctg tttagctaga atgtataaag ttgagttatg gtctgatata cattttgaaa
    14761 tagtcgataa ctatttaaaa gaattagcat ctttaggaga aaaagttgca acagttatag
    14821 tttcagatta tccatgggca gggcaaagtt gttataaagt ttataaaaat ccatcaaact
    14881 tatatgaata taacatggta agtgtatcaa aaggattgga tggaaagatt aaatgtaact
    14941 ttgagagtat ggataggtat ataggcatag ctgataagta taaaatggct aaggagatag
    15001 atttatttgg tctattaggt aattggtgtg ctggtgagtt tggaaatcct gtagaaggtt
    15061 ataaggaccc tataagagta agatattttg atgaaaagga taaagtattt aaatttataa
    15121 acaatacaaa tgatttaaaa gaatatatag gattagtatt aaatcattta atagagtgtg
    15181 gattatggga cagagtaagg attatagctg atgaaccaaa taatccagaa gtagtcaaag
    15241 agtgtataga atttataaat tctactgtag gtactcatca agttaaatat aaatcagcaa
    15301 ctcacgacca aaacttttta gacagagcta aagatgaaat tgatgatatg tcaataaatt
    15361 taaaacttac tatacaaaat tataaagata tagaaagtct taagaaaaaa atcaatgaca
    15421 agggtgggat attgacttgg tttgtatgct gttttccaga aaaaccaaat agttttttaa
    15481 gttctccttt tgtagaaaat agaataatag gatggtatac ttattacttt ggtcttgatg
    15541 gatttttaag atgggactat aatttatgga cagaagaccc ttggaaagat tcaagttaca
    15601 aattccctat ttggaaagct ggagatatgt tctttgttta tcctggaaaa gatttaaagc
    15661 cagttagaag tgtcagaatg gagaatttaa gatttggaat acaggatttt gagttattta
    15721 caatgttaga aaaagaaaag gtaagagagt atatagaagt tgagcttatg caagaattat
    15781 taaataaaaa agaaaatgct gaaattaaag gttttggtga catagaacta ggatattcac
    15841 ttgataattg gagatatgat aaagttaaaa aatatgtatt agagttgtta gacagagtat
    15901 aaaaaatata agagtgtctc aaaatgaact ttttagttca tgaggcactc tttttgtatg
    15961 aaatcaatat agtttcattg tttcaacaat gaaaaatagg attatttcta ttctaagaca
    16021 gccctttagt tttttagttg tattcaatgt tttaaaacta actataaacg ctataaagtt
    16081 tataaatgac aataattata tataacatga tgtactttaa tttgctataa tagggattga
    16141 ataatattat atggaagata acattgtttg gggggattaa tataaaaaga ataggtatta
    16201 ttataatgtt ttttttaagt atatctttta ttggatttta tcttgtaata agagataaca
    16261 gtgatacagt taagatgcaa cgagggataa atataggtaa tgcactagaa tcacctaagg
    16321 attttccgtg ggatgtaaaa atgtcaaata agttttttga tgatataaaa gatgcaggtt
    16381 ttgacacagt acgtatacca gtaaggtttt ctgattatac gtcagattct gataatttta
    16441 aaattgatga agaatttttt aaaaagattg ataaatatgt agattatgca ttggataaag
    16501 acttaatagt tgtgttggat ttacaccatt ttgaagaaat tatgaaagaa ccaagagttc
    16561 ataaagaaaa gtttttaaaa atatggcaac agatagctaa taggtatcaa aaatatgata
    16621 aaaaattggt atttgaatta ttaaatgaac ctaaagaaaa tttatcttca caattactaa
    16681 acgaatacat tgaagaagca ataaagataa ttagaaaaac taaccccaaa agaactatta
    16741 tagtaggacc atataatttc tatcaaattg attatttgaa tgaattaaat attcctaaag
    16801 attctaatat agtagtttct tttcactatt acgagccaaa tgattttgca tttcaaggaa
    16861 atatctatca taagggattt gagcacttaa gcaatattac ttgggaagga acaaatgaac
    16921 aaatggatta tttaaaaaaa aggtttgata ctgtggaaaa ttgggcaaat aaaaataagg
    16981 taaaaatctt cttgggtgaa tttggaataa caaaagaggc accagaagtt tcaagaagag
    17041 catggataaa agctgtaaga gaagaagctg aaaaacgaaa cttttcatgg gcttattggg
    17101 agcttgcatc tggatttggt atatacaatc aaattgaagg gacttgggat agagatatat
    17161 taagtgcatt aatagaaaag aggtgatttt atgaagaaaa attcaaaaaa gatacttata
    17221 actttaacta ttattactaa tgtcatatat atattgtgga gaatttttta cacagtacct
    17281 aaagaagaag ggaagtttgc cttaatatgt gccataatac ttctttttgt agagataatg
    17341 ggtatgatgg aaatgtttgt gcattattat ggtatgtcaa atattgagta tcctgaaaaa
    17401 ccaatcataa gtgaagaact ttatccacat gtagatgtat ttattgcaac atataatgaa
    17461 tctgttgatt tggttagaaa aactgtaaat ggatgtattc atatgcagta tccagataag
    17521 aaaaaggtac atatatacat atgtgatgat ggaaatcgtg aagaaatgcg tattctagca
    17581 gaaaaaatgg gtgtaaatta cataactaga actgaaagag aaggagcaaa agctggaaat
    17641 ttaaataatg caatgcaaca tacaacttct cctttgattg ctacatttga tgcagatatg
    17701 ataccaatgc atgacttttt aattgctaca gtaccatact ttttaaaaaa tgagcaagct
    17761 aaaaaagatg gagaaaaaga ggagtatgaa aaggttggtt ttgtgcaaac tccacagagt
    17821 ttttataatc cagatttgtt ccaatttaat ctgcactctg aaggaaggat tccaaatgag
    17881 caagattatt tttacagaga tattcaattg gctagaaata ggacaaattc tgtaatttat
    17941 ggtggtagta atacagttat ttcaagggaa gcacttgaag aagttgatgg attttatact
    18001 tattctatta cagaagattt tgcaacaggt atcttaattc aaagtaaggg gtatagatgt
    18061 tatgctattc cagaagtaca tgcatcaggt ttatctccta cagatttaaa aagtcttatt
    18121 aagcaacgtg aaagatgggc acgtggatgt atacaaactg gtagacgtct aaatattttg
    18181 tttaggagag gtttaggttt ttggcagaaa ataagttata tttcttcaat tacttattgg
    18241 tatgcaagta taaaacgctt tgtatatata atggcaccta ttttattttc tgtgtttaat
    18301 gtgattgtag taaaatgtac gttacttcaa gtattggtat tttggttgcc aatgtatatt
    18361 ttaagtagtt tatctcttaa aatattttct caaaatataa gaaatactag atggaccaat
    18421 atatatgaaa ctataatgtt tcagtcttta atgccagcag taatacttga aacgtttgca
    18481 atatctaaaa ataaattttc agtaacaaat aagagtaaat tagaagaaaa tagaatgtat
    18541 aagtttttac agggaatacc ttattttatt tacatggtct tatctatcat aggaatttta
    18601 aaaatgtttg tagcaatatt taaaatgagt tcaatgacat attcagttgt attattttgg
    18661 ttgattggaa acctttttaa ccttgttatg gctacattat ttatatcagg gagacagcag
    18721 ttaagaaagt ctgagcgtta tattgctgaa attgatttta agttaaagca aaattcttat
    18781 gttctttctt caaaaacaat tgatatatca gaaaatgggt ttgcattttt gctagaaaat
    18841 ccagaatata tttcaccaga agaagagttt gaggtagagt ttagagaaaa atctggaaat
    18901 gaaatgtata tagccaatat gaaagcaaag attgttaatg ttgtggaagt aaattcaaag
    18961 tggaaatatg cagcttatat tacacatatt gaagatagtg aaatagataa ttggatgtgt
    19021 attgttcacg atagaatacc tactttacca atgacaatat ctaatcaact aggtttcttt
    19081 gatgatttgc aaattaatgt gaaaaaacgt attgaaaaaa ctagaacatt atcacgaaga
    19141 agtccaagaa tcaatatgaa ttttcagatg gatataaaaa atacaggtaa attaagaata
    19201 gtgaatttta attatcaata tgtattatta aattttgaga ataaaagtat ttatcctaaa
    19261 gaaatagcat tggaaattaa tgaaaatatt gtgttagaat gtgatttgtg tgagggtaaa
    19321 attgatgaaa gaggtatctt atataaagta aataacattg acagcataat gcaaaatttc
    19381 tttttaagag atgaaatgat ggattggata ttacaaaata agacaattct tgtttcaaag
    19441 cctagtgaaa agaaggaaaa aagtatagat gagtttgaac ccatggaata tatttaggag
    19501 agatgaaatg aagactttaa aagtgttttt ttgttctaca aaatatctta ttttggtgtt
    19561 tatttttttt ataaatacta cttttataag ttcagcaaca agtgataaaa ttttagattt
    19621 gtcctttaaa aagatagaga ctgacttaaa ttctaagatt acatatgaag atacaggtgt
    19681 aaaaatagaa acggatagtt ctaaaagcga taaggaacgt tatttatata tttaccaaaa
    19741 tataaaagaa aattggagca tgtataataa tttttatatt gaaattcaaa ataaaaataa
    19801 aagttctcag agaataaatt taagcataca aagtaaaaat atgcttgaat ttagattaaa
    19861 agaaggtagt gaagtatttt tagaaggtaa aaatataata tattcagata aaataaaaga
    19921 aggatgcata gaagtaccag gtgaatttga aggcaaaatt tatgtgaact ttaatagcct
    19981 tataaatgag gaaagtaatg ttgtgctgga ctcaaatatg ctttcaaaca ttgttagttg
    20041 gggaattacg tttattccat ctgatgaaga acataatatt gttattataa aaaagatatc
    20101 tttattgtct gaagagaaat taagattttt aaataatatt aaaataattg gcgatgaaga
    20161 agtacaaata ccagtattgg gtcaaagtat aagtcagtat gaagtattag gtcttaaaag
    20221 tgattctaaa attaagtact ctcttatggg aaaacaagat aatgtaagca tatctcaaaa
    20281 aggtaaatta acattaagta ataaagccaa acctggacaa attattttac aagttaatgt
    20341 tgatgataag tttaaaattg gaaagaaaat aacattaact gagtcttgga gtgttaataa
    20401 aaaagataag gatggtgttc catatacatt agtatatcct gagcaatcac caacagtaca
    20461 agatatgaaa aaaataaact ttatgaataa catcataacg tttgttcgaa ttttatttgt
    20521 atctctagta ataatatgct ttggaatata tttatattgg aaaaaatgtt caaaaactaa
    20581 gtaaagagag gaggttagat tgtgttattt tcaagtttaa tttttttatt ttatttttta
    20641 cctataactt tggttttata ttatgtattt aggtttaacc gaacgataca gaacatgata
    20701 ttgcttgctg ttagtttatt tttttatgct tggggagagc caaaatttgt tgttatcatg
    20761 atagtttcca taataatgaa ctatatattt ggtttactgg tagatagata tagggaaagt
    20821 aaaataaaag taaaagtttt tttagttttg atgtgtgcat acaatatagg tgttttattt
    20881 atatttaaat atctagcttt tgcgctaaga aatattagtg aacttattag tactgaattg
    20941 actataccta gtattgtttt acccatagga atttcatttt ttacatttca gggaatgtct
    21001 tatgttatag atgtatatcg tagacatgga gaagtccaaa agaacccttt ttatgtggga
    21061 ttgtatatag catttttccc acaattaata gcaggtccta tagttagata tgagagtgtg
    21121 gcagaacaaa ttctcaatag aaaagaaact tggaataaat tttctattgg gacttgtagg
    21181 tttataacag gtctaggtaa aaaagtatta atttcaaata acatggctat agtagcagac
    21241 tatatttaca ctatgaatag tcaaggagaa atagctgcat cacttgcatg gcttggttca
    21301 atagcctata ctctgcaaat attctttgat ttttctgctt attcagatat ggctataggc
    21361 ttaggtctta tgtttggttt taaatttgaa gagaacttta attatccata tatatcaaaa
    21421 tcaattagtg agttttggag aagatggcac atttctttgg gaatgtggtt taagagctat
    21481 atatactttc cgcttggagg ttctagagta gcaaataaag atattatgat tagaaatatg
    21541 tttatagtgt ggttatttac tggaatatgg catggagcag aatggacatt tgtaatttgg
    21601 gggattttaa actttgtttt cctaattata gagcgattta taatgtttga aaagattgaa
    21661 aatcacaatt ttataaaaca tatttatgct ttattagtag ttaattttgg gtgggtatta
    21721 tttaggtcac ctaatttaaa agaagcatat aattatttta aagctatgtt tggaggcaat
    21781 ggagctatat ggagtgatta tacatatatg tttttaaaag aatactgggt tttctttata
    21841 tttgcattta tttttagtgt acctattgca aagaaaatca ataagtttgt tgttgaacaa
    21901 gctaaatata gtatgatatt taacgtattt tatccaatct cagctatttt attgtttttt
    21961 atttctgtga catatttagt aactggaagc tataatccat ttatttattt taatttttag
    22021 ttgggaggaa aagtatgaat agatttttta aagtaagatt aataactgca attatgttct
    22081 taattatttt attcgctttc tctatcttta atgtttgtta ttctggaaaa gatacgatac
    22141 aggaaataaa aaattcaata gctgaaaaaa gtagtttaaa agatacaata tcatctattg
    22201 ataatacagt gaatgacaaa gtaatataca aaaatacgtt cattgaaact tatggctatt
    22261 tacaaaaatt aatgggtaaa aatgagtttt caaaatttag tgttgtaaaa gatacttctg
    22321 gaaagatgca ttatacatat tttgctaaag ctccaaatcc agttggtgtg atagcagata
    22381 gggtaagaaa attttctgat gaaatggaaa agcaaaacac taaattggtt tacctaatga
    22441 ctccagataa gtatataaaa ggagtgacaa aatttccaaa aggaatacct tattcttata
    22501 gtaatgagac agcagataat tttttaaatc aactaaaaaa gtataatgtg gactatattg
    22561 attttagaga aaatatttta aagagtggta ttccaaaaga tgatttattt tttaaaacag
    22621 accatcactg gaaagtagaa acttcttttt gggcatttgg agaattagtt gagcaactta
    22681 ataaaaaata caatatgaat ttagatgaaa atcattatta tagagataag gacaattata
    22741 attcaattgt atatcctaag tcatttcttg gttctatggg aagaaagact ggtatcttat
    22801 atggtggtat agatgatttt acattaattt atcctaaatt taagactaat tatacttatt
    22861 atacagattc taaatctcaa aaatttgaat taaaaggaag gtttgaagag agtttaatat
    22921 tatcatatcc ttttaatgca gaccttgatt taatggatgg tcaatcagat aagtatttta
    22981 catatttatt ggggaacaga ccattagtta aaataaaaaa tatagaaaat ccagatggat
    23041 taaaagtgct gtttgttaaa gattctctaa ttgtaccaac agcagcattt ttttcctctg
    23101 tttgttcatc tatagatatg atagacccta gatattatga tggagatata ctagaatatg
    23161 caaaatccca tgactatgac tttgttttta tatctgtata tccccaaaat cttacaaaag
    23221 aattttttcc gttttgtgag taaatttcta aaaaagtttt ctgataaaat aaatggctaa
    23281 ctggtaaaaa gttagccatt tattttgagt gttattctaa gaaatatttt ttttactaaa
    23341 tacttcttta tctatttttc caagtgattt tcctacagta attgatgata ccatagcacc
    23401 attaacattt atcatagtac gtcccatatc tagtataggg tctattgcaa gtataccacc
    23461 aactaatgga aaataagaac caagaccaac tccagagata acaactgaaa cggccattgt
    23521 agcagttcct ggaagaccag ctattccaaa cgaactaatt gttataacaa ttaacaacat
    23581 aacataaaag ctcatatcca tgtttactcc agccatattg gcaagtgtta cagccattaa
    23641 agcaggatat atacctgcac aaccattcat acccatattt gcacctaaac ttgctgtaaa
    23701 gcttgataca ccctcatcaa ctccgtgatt ttgtactaga ctttctatcg ttacaggcaa
    23761 tgttcctaaa ctagaacgtg aagtaaatgc cagaagtaaa gcttcagttg aattttttat
    23821 ataagtaatg ggatttagac catttaaagt tataaaaatt aaatgtataa taaataatat
    23881 tgcaactgat atataaagag ctaatataaa ctgtaaaaca gatactattg aactaatacc
    23941 ttgtgaagtt attgtatttg caagcagagc aaccacagca taaggcataa atttaataac
    24001 agtcatagac acacttacaa ttattttgta agatgcttct ataaaaccta taaatggttt
    24061 tattgtttca aaatgttttt tacttaatct ctttattgag gtagctataa atgtagcaaa
    24121 tattattgta gctacaatat ttccatttgc catagccatt acaggattag atggtagtaa
    24181 tcctcttaaa gtatccacaa gagagtttat ttctttaagt tcttgatttt caactaaagt
    24241 aggcatatta gctcctacac ctaacttcat tatatttgca acgataattc caacaattgc
    24301 agctatagca gtagtagcaa gaagcatacc taaagattta aatgtcagag ttccaagatt
    24361 gttatctttc atatttataa ttacttttat aatggataag aaaactaaag gtactactaa
    24421 catttttaat aagtccataa atccagaacc aaataggcta taccacttac ttacttcttg
    24481 tagccaaatg acttgagttg tatcattagg aaaaccagca aaagcttgta ttatgattcc
    24541 caatatgagt cctagtactg tagcatatat tgttctcaat gaaaatttta tttttttctt
    24601 ttcaaattgt ctaacaataa agaacgtacc tagaagtaat attatgaaaa aaattgtttt
    24661 aaaatctgta atcataagaa attgtgataa aaatgaactt tccaatattt ttctcctttt
    24721 atttaaaaat aaatttttct aagtttaaat gatatcaata tattaaaaaa atgtcaatat
    24781 tatatcacaa tttacttaaa tagagtaaaa caagttgatt ttgaaaaatg tgattaagta
    24841 cagaaaaata gggtataata aatgtttaaa gaatttgaat tgtggtaaga aaataaaaaa
    24901 tagcttttat aagatttgat ttcaaattaa aatgtgttaa ataaataact aaaaattatt
    24961 ataaaaaatg ttttcataga atatatatca aatgaaaagc ttaaaataag tgaagaaatt
    25021 aactagacat caaaggagag ataaaatgag agtaataata attggtggtg ttgcagctgg
    25081 tatgagtgct gcagcaaaat taaaaagaat aaaacctgag tatgaagttg ttgtttatga
    25141 aaaaactgaa atagtatctt ttggtgcttg tggattacca tactttgttg gtggattttt
    25201 taatgatgct gatgaattat tagcaagaac accagagaaa cttagagaag ctggaattga
    25261 tttaaatata tttagagaag tcgttgaggt tgatagtgaa tctaagaaaa taaaagtaaa
    25321 aaacataaaa acagatgaaa tatatgaaga ttactatgat aaattgatga tagcaactgg
    25381 agcaagaagt attatgcctc ctataaaaaa cataaaatta aagaatgttt ctacattaaa
    25441 aagtttatat gatggagaat atttaaaaaa acttttaagt aatgaagata ataaaagagt
    25501 gacaataata ggagctggtt ttatagggtt agaagcagtt gaggcatgta agaaattagg
    25561 taaagatgta gatgtaattc agttagaaga tagaatattg ccacaagtat ttgataagga
    25621 aatgacggat gtattagaag aagaaataaa tagacataat gttaatttgc acttagatga
    25681 aatggttgta gaactttgtg gagaagataa agtagaaaaa gttataacaa acaaagggga
    25741 aatagataca gatgtagtaa taattgctac tggcgtgaga cctaataccg agtttttgtc
    25801 aaatactaat ataaaaatgc taaaaaatgg agctatagta gttgatgagt atggaagaac
    25861 ttctgtagaa gatatatact ctgctggaga ttgtgcaact ataaagaata tagtaagtaa
    25921 tgaaaatgta tatgttcctt tagctactgg agctaataaa ttgggtagga tagtaggaga
    25981 aaacctagca ggaagagaaa tatcttatca aggttcacta tcttcaagct gtataaaaat
    26041 tatggatatg gaggcagcat caactggaat tacagaaagg caagctaaag atttaggcat
    26101 aaatgtcaaa tctaaattta tttctgatta taatcaaact cattattatc caggaagaaa
    26161 taaaatatat gtaaagctta tttatgatgc agatacaaaa gttattttag gcggacaagt
    26221 ggctgggttt aaggatgctg tccaaagagc aaatgtattg gcagctgcta tctttggaaa
    26281 aatgaccaca aatcaacttg gaatgttaga tttatgttat gctccaccat ttgcaagaac
    26341 atgggatgtt ttaaatgtag ctggaaatgt atctaaataa tatttagatt atagctaata
    26401 tagttgacaa gcaattaagg gaatgaattt acctaaacta tttttatcat ggtaaaattc
    26461 attcctaaat ttttattcca aatttttatt ccaaattttt agaaataagg attccattaa
    26521 attattcatc aaaatttaaa cgcaagctta atctatcttc tccaagaata gtattttcaa
    26581 aaacattagt agcattttct aaatacatac taggtatgtc taaagaagga ctaaaatgag
    26641 tcagtaaaag ttgtttgaca tttccaagtt ttgcaagatt agcagcttct ctaaaagtca
    26701 tatgtttgtt tctaacagct tttgatatat ctaaatcatc accatacatg gcttcacata
    26761 taaataaatc actatctttt atgaaatctg gtattgattc tataggtctt gtatcggttg
    26821 taagacttat cttaactccc tttctcaatt ctcctaagac catatctgga gtgtattgtt
    26881 ttgaatctaa aactatattt tgaccttctt ggagtttgtt ccataatatt ttaggaactt
    26941 cgtttttagt tgccttatct atatcaaatt tagggtttct tctaaaatta aaactgtatc
    27001 ctaaacactc tatagagtgt tctaaactga ttgttgaaat ttctaaattt cttaaaactt
    27061 tattatctaa agaaaaattg ccttgagggt tttctataat ttttaaagtg tatggtacat
    27121 attctactaa atttctcata gaacgtatac aatcgactat tccaactgga ccaactatag
    27181 ttaaatcact tgtacgacca ctattcccaa ttgttgataa aagtcctaaa agtccaaata
    27241 tatggtcacc atgtaaatgg gttatacaaa tcaagtctat atctttaaag ccacattttt
    27301 ttagcttcat agatacctga gtaccttcac cacagtcaat tagtattttt ctacctttat
    27361 agtttataaa tacagaggat agaaatctgt ttggcatagg tacatttcca ccacatccaa
    27421 gtaaagttaa atctatcatt ttttcccctt tctaaaatta acacaaatct gttgtttgtt
    27481 tttattatga tatcctatta taattatttt aacattaaat ataacaataa atgtgtattt
    27541 gagtcaagaa atattgtcaa ataaagtttt tatatagcaa tgtataaaaa tttggaaaac
    27601 gaaaagtgaa aattatattt aggagtattt aaaggaggaa aatttaatga aagtattaaa
    27661 gaaaattact attttgtcat taattacatt aaccttagtt ggttgtagta acggagaaga
    27721 gaaaaaagtg gttgaaggtt ctaagaaaac tcaaaattct aaaaaagatg aacagcctat
    27781 aatagtacat aaagatgcaa atatattgaa agataggtat gagccaccaa aaggatttaa
    27841 aagagttgat gtagaaaaag gtagttttga atcatttctt aggaatacta agctaaaaaa
    27901 ttatggagag aaagtaaagt attatgatgg aagaactaag gaatcaaaag gtatttatga
    27961 tagtgtattt gatgttgata ttggaaatag agatttacat caatgtgcag atgcaattat
    28021 gcttatgaga gcagaatact tgtatcagaa taaactatat gatgaaataa gttttgattt
    28081 tgttgatggt tttaaggcga agtattcaaa atgggcacaa ggatatagaa tttcagtaaa
    28141 agatagtggt tcaagctggt ataaagcaac tgaatattca acatcttatg agagtttcag
    28201 aaagtttatg gatatagtgt ttgcatattc aggaacgtta tctcttgaaa aagaattgga
    28261 gcctgtaaaa gttgaggata tgaaaatagg agatgtgttt atagttggtg gaagtccagg
    28321 acatgcaagt ataattatgg atatggcaga aaatgaaaag actggtgaaa aggtatttat
    28381 gatagcccaa agctatatgc ctgcacaaca aacgcaactt cttattaatc gtaaaaatcc
    28441 aaatttgagt ccttggtata gtttagattt tgaaggtgat ttgacaatac ctcaatggac
    28501 ttttaaaaga gaccagttga aaagatttta aaataataat tttatataat aaaagaaaaa
    28561 tatagatgtg aaaaaatgta attagattaa aactaattgg atatggacaa aattaaattg
    28621 atagataaat attaaaaatt acactaatta atcttactat agtttaatat ttatctaaaa
    28681 agttttatgt aaaatataaa ttttatatta aagctaaaac taatttgagg aggctaattt
    28741 atattgaaga ttagaaggta tttaattttg gtaattataa ttctaattac aacaagtcag
    28801 agttttattt cttatgggct agagcgattt gaagaaataa ccatttttca cactaatgat
    28861 atacatggaa gatatgctga aggtgatgac catatacaaa taggaaatct agccacattg
    28921 aaaaaagaaa ctccaaactc aattttagta gatgcaggag attgtttgca tggacttcca
    28981 atagtaaata tggatagagg gaaaagtgca attgaattaa ttaaatgtgc tggatatgac
    29041 tatattactc ctggaaatca tgactttaat tatggaaaag atagactgtt tgagttatct
    29101 aaaagtgcta atttaggaga gaatacttta aaatttttat catcaaatat ttttgaaaat
    29161 gggaaaaaag tttttgatag taatgatata aaagaaatag atggaataaa aataggattt
    29221 tttggactct caacccagga aacaaagagc aaaactggat ttaaaaatgt ggaaggatta
    29281 gagtttaaaa atcctataga aagtgcaaca gaacaagtaa atgaattaaa aggaaagggt
    29341 gccaatgtaa tagttgcaat atgtcatatt ggtacaaata gtttaagtaa gccaaatagt
    29401 atagatatag caaatgaggt tcaaggaatt gatttgatta ttgatggaca cagccacact
    29461 aagtttgaaa atggtaaaca agtgggtgat acattgattg taagtgcagg acaatattta
    29521 gaaaatattg gcgagataaa actaactttg gatactaatg atgtagaaga tataaaaata
    29581 gaaaataaat cagctagatt aattggaaaa gaagaagcat taaagtataa gcttgactcc
    29641 gttgtaagta aaaaggttga taaaataaaa gaacaacaag aaaaaatatt agataaagtt
    29701 ataggggtaa ctacaaatac tctagatggt tcatatgaaa atgtaaggac aaaagaaact
    29761 aatttgggta atttaataag tgacatttta ctagataaaa ctaaggctga tatttcttta
    29821 tttaatggag ggaatattcg agatacgata gaaaaaggca atataacaag aagagatatt
    29881 gtagatgtat ttccttttag caatactata gttaccaaag aacttacagg agcacagatt
    29941 aaagatgtat tggagcatgg agtaaaactt tatccagaaa aaagttcagc ctttttgcaa
    30001 gtgggaggaa tttcatatta ctttaatcct aaacaaaaag aaggcgaaaa aataacaaat
    30061 attcaaaaag aaggaaagcc attagattta aataaaaagt atgtagttgc aacgaatgat
    30121 tatatagcat caggtggaga tgaatttcct tgtttttcaa aagagccagt actaaaggaa
    30181 tttggaaatt tagagagtgc agtcattgaa tatatagagt acaaaaaaga aatatcaaaa
    30241 aatgtagatg gaagaattgg tattaaatct aaagatgagt taataagcaa gaatcatgag
    30301 agtgatttga tattaagtga aaatgaaaaa aatatcaatg ataatgaaga gaatctatct
    30361 aataatataa aaagctctga tagtgataaa aataaaagct tagataaaac aagttctaga
    30421 gaaagttcta atctaggaat atctaaaaga gaagctaaag aaaaatctcc taaaacagga
    30481 gatttaggtt tttcaaatag tataattata tttatagttt caagtacatt gatatgttta
    30541 ttgaatttta atcaaaaaga gttaaaggat aaaaagtcaa aataaaaatt aatgagcaaa
    30601 aattagtaga tagtaattta attttagata atagtaaaca gcaaaggaaa ttagttatat
    30661 aaatactaat ttcctttgtt ttatgtttta acatgattac ttttaaataa aattgtattt
    30721 tatgaaaaat aattatacta aatggcatag tattatgggg atttttagtt tttattcaag
    30781 taaatgaaat catattaaca acttctgata tatttacaaa ttgataaaaa aatgatttta
    30841 ttagtatgat ataatttagc tagtatgtat gataatggag gatataggga tgaataatat
    30901 attacttttg gaagatgaca aatctttaaa tagaggaatt agttttaaat taaaaaaaga
    30961 aggatacaat gtttttagtg catttggtat agaagaagca aaaaatattt tcgcaaaaga
    31021 agagatatgc ttaattattt ccgatatagg tctacctgat ggtagtggtt ttgacttttg
    31081 tgaggagatt agaaagaaaa gtgatgttta cataataatg ttgacagctc ttgatgaaga
    31141 ggtggatatt gtcacaggat atgatttagg tgcagatgat tatatcacaa agccttttag
    31201 cttgatggta ttaatatcaa aagtgaatgc gcttatgaaa agagtaaata cagtaaaaaa
    31261 ttatactcta ttagtttgtg atgatttatt cttttattat atagaaaata aattaattat
    31321 aagaacagat gataaggaag aagagataat tttaagtaag acagagacaa aactattaaa
    31381 atatttgatg gaaaattcta tgcagacatt gactaaagag caactgttag aatctttatg
    31441 ggatagtagt ggtaattttg tagatgataa tacgattgca gtaaatataa gaagacttag
    31501 acaaaaggtt gaaaaaaatc catctgaacc taaatatata aaaacagtaa gaggggttgg
    31561 atatatgtgg ggagaaagga gtataaaaaa atgctaaaaa atatgaacct caaagatggt
    31621 ttaagtgtga aagatgatta tataatgaga agagttattg tatattctgt tttatctgta
    31681 ctaatagtaa cccttgtatt tatacttttt gaatatagca gattacataa attgtatgaa
    31741 catcaaataa aaaatactca aaatgttgct ggaatattga ttgaaaaata tcctaatgat
    31801 gaaatagata tagtaaattc tatttataat tctgattata gtaaggtgtc tactggagaa
    31861 aaggcattta agaaatttgg ctatggattg gaaaataaaa tgtcaaatga taaaaatttt
    31921 agcttatact taaaatcttt ttttaaggaa tcatttttta tttttttaat gatgtttata
    31981 gtagttatag gtataatata ttattttata agatacataa ataaaagatt gagtaaaatt
    32041 tattttatag tagataagat gtcacaagaa aactacttga aggacactac tgagtcagaa
    32101 gaaactggtt atctagtaga atataatgaa tactttaaag aaggcatatt tagtaagata
    32161 aataactgtc tatatgagct taatagaagt ttaaagataa agtttattaa attagaaaaa
    32221 gaaaaagaaa gtgtaaaatc attagtaaca gatatttccc atcaattaaa aactccttta
    32281 gcatccttaa aattatacaa tacattactt atagaagaag aattagaaga agatgaagcc
    32341 aacgagttct tattaaccaa taaggcttct ataaataaat tagaaaattt aataaattct
    32401 ttagtgaaca tatcaaggtt agaaattagc atgataaaca taaaaaaaga agataatgat
    32461 ataaaaaata ctattctaaa tgcagtaaaa agtgttaccc ctaaggctaa gagcaaaagt
    32521 ataacattaa atttaaatga atttaacagt agaattatac cacatgataa aaagtggact
    32581 gaggagagta tttttaatgt attagacaat gctgtaaagt atacacaggt aaatggagaa
    32641 ataaatatat ctgtagaaga aactgctaat ttctttaaaa ttataataga ggataatggc
    32701 atagggattg aaaaaagtga gtttaataat atatttaaac gtttttatag aggttctgct
    32761 gatgaaattg aatctgtaga aggtagtggg gtaggattgt atttaagtag aaagatttta
    32821 gaagaacaag gtggaaatat tatagtttct tcaaaagtag gaatagggag caagttttct
    32881 ttgtttctta caactatgta atattaaatg aaaggaaaat gtaagcctct acatttatta
    32941 tataagtata aacagctcca gaaataaaaa attaataaaa tatggagtaa tataacttag
    33001 aatgagagga taattttatg aaaatactat atactgaaaa tttatcaaag cattatggaa
    33061 aaggagaaag cttagtaaga gctttggaca atgttgactt agaaataaat gaaggtgaat
    33121 ttgttgcaat aataggaaaa tcaggttctg gaaaaagtac acttttgcat atgattggag
    33181 gtcttgatat acctacatct gggaaggtgt atatagataa taaaaatata tttacattaa
    33241 aagaagaaga acttgctgta tttagaagaa gaaaaatagg gtttatattt caaagctata
    33301 atttaattcc atctcttaat gtatgggaaa atgtagtgtt gccaatcggt ttagatggta
    33361 gagaggtaga tgaacctttt ataaaagagc ttttaaaatc attaggactt gaaaataaac
    33421 acgacgtttt accaaataca ttatcaggtg gtcaacaaca aagggtggca atagcaagag
    33481 cattagctac aagaccagct ataatacttg ctgatgaacc aactggaaac ctagattcta
    33541 aaacatctga tgaggttatg agtatattaa aatctatgtc taagaagtac agtcaaactc
    33601 tagttatgat aactcatgat gacagtatag cccaaatggc tgatagagtt atattcatag
    33661 aagatggcag ggtgtcaaag gttggtgata aaaatgatta atactactaa aatagcaaaa
    33721 agtaatttaa agcaaaataa atcaaaaagt attctaataa taattactat aatactttca
    33781 accttaatgc tttcttctat tggaatttat attgttgatg caggagcata tcaaaaagaa
    33841 aatactataa agtattcagg taattatcaa ggtatattag caaatgtaga cgaaaaacaa
    33901 gctgatatat tgagtaatca tgcagatgtt gaattaactg gtaaaatgaa tggagtaggt
    33961 gttgaaaaac tggaagatga ttcaaatatt tctctagcat acatgaatga agatgctttg
    34021 aaattaaata gttttgaatt tatcaaagga aaattgccat caaaagagaa tgaaattgtg
    34081 ttagatagtg gagctttaaa ggcacttggt tataaaaata aagatttagg tgaaaagata
    34141 aaaatatctt ataatgacta taaaagtgat aaaaaaattg aaaaagagtt cattataagt
    34201 ggaatcttaa aaactagcga aatatctgaa gcaggtaaat attattatgc tattatatca
    34261 gaatcatata tgagaaatac tagagatatg tcacaagaag attttaatat atatgtgaaa
    34321 ttaaatgata aaagtaattt atcgatagaa caagcaaaat caaaattaaa taagattgct
    34381 aatgatgtag gattggacac tattaatgta gctgtaaatg aaaactatat aaatgcttta
    34441 aaaccagata tggaaacaat tatgggtgga gtatttgtag ggttagtaat tgtattgtct
    34501 agtattttag taatatacaa tatattttat atttcaatag ttacaaaggt tcaagaattt
    34561 ggaaagctta gagcaattgg tgcaactaaa aaacaagtaa aaaatatagt atttaaggaa
    34621 gggttcatat tagccggaat agcaatacca ataggaataa ttttaggcta tgtacttgca
    34681 aatattataa taaaatcatt tatggatata gatgtaaaat catcacgatt accagttata
    34741 ttatcggttg tggtaataag ctttatatct gttgttttat cacttttaaa accaatgaaa
    34801 gtagcatcaa aagtatccat tgttgacgca gtaagatata caggaaataa aataagtaat
    34861 aagagtaaga gaaaaggata taaaaatata aatctaaaga gattatcaca tgccaattta
    34921 gaaagaaata aaaaacgtac ttacatgaca ttggcatcac ttatacttag tggaactata
    34981 tttataacag tatcaactgc cttagaaagt tttgatgctg agaaaatggc aagagaacac
    35041 tttccttatg atatagaagt tagactatct ggttatgaaa tgaacagtga taaaaatcct
    35101 aaagataatt tgaatatttt acaaatggat aatcctctaa gtaaagattt ttttaatcaa
    35161 ataaagaaca tggaaggaat taaaagaata gaatctgcaa gaagtgtaaa aattggtatg
    35221 gaggactatg atgttgaatt taagtatgac cttttacaaa gtataaatga aaatgatgta
    35281 aaatcattaa acaagaatat aatagatgga aaaataaatc ttgaaagact tcaaactgga
    35341 gatgaaatag taattactca tgttgataca gcaaaagaaa tgggagtaaa agcaggagat
    35401 aaaataagat taactttata tgatggagat aaaaagataa aaaaagaatt taaagttcaa
    35461 gcaatagcta tgggggttcc aagttttggt atagggaagg attttattga tagaactttg
    35521 aaatacgatt caactagtag cttgggaata tatacagaag aaggaaagta tcaagaaatc
    35581 aaagatagta tcaagaagat tgctaaaaat aatggatttt tagagactga ttttatagat
    35641 agtagaattg agtcaaataa ggctactatt tcatttataa agataatggg atacacgcta
    35701 acaggcataa taggtgtgat tggatttatg aatcttgtca acacaatgat aactagcata
    35761 gttactagaa aaaaagaact tggtatgctt caagctattg gtcttactaa taaacaatta
    35821 gtgaaaatgc ttaattcaga ggcaatgtat tacacttctg gaatgatgat aggaagtatt
    35881 ctatttggag gtatattagg gtatattgct gttatatttc taaaaaagac tggattatct
    35941 tatgcaacat attctttgcc aatagtacca atcttgttga tgattgtgtg tattcttata
    36001 gctcaattta taacaactta tttgatagga cgaagtttta ataaagaatc tcttattgat
    36061 agagtaagat acagtgaata aaatacaaat atttatggtt agtttaacta gcaaaaaaca
    36121 tataaaaaaa tatagttaaa gagattcata attaaatagg ttaaagcata gtacagagca
    36181 aacaatttaa gcagatgttt gctctgtatt ttattatgta atcaataata tatctaatat
    36241 actgatatta ttcatgttag aaaatgtacc ggtacaattc taaaaaagta taaaattgta
    36301 ccatataaaa tgaaaatttt gtataataat cgtatagaaa ataaatagga gctgatatat
    36361 atgaataaga agattaatta tgcaccagga ccaactgaaa ctagggaaaa tgtaagatta
    36421 gcaagagcag aaaaaacaac aaatccagat atagatattg attttgttga attttataaa
    36481 aagacatgcg agaagtttgg taatataatt ggaacaaaaa acgatgttta catattgagt
    36541 ggtgaaggaa tattaggtct tgaagcagca tgtgcatcac ttacagaaaa aggagataga
    36601 gtacttgtaa ttgacaatgg tatatatgga gaaggtttta aagattttgt aacaatgtat
    36661 ggtggggagt atgtgctttt ttcaagtgaa tatactaaaa gtatagatat tgatgaattg
    36721 aaagcatttt tagatgagga tagtaatttt aaatatgcaa cagtagtgca ttgtgatact
    36781 ccaactggcg tattaaatga tgtaagcaaa atatgtccat tacttaaaga atatggaata
    36841 ttaactgtag ttgattctgt agcaggtatg gtaggagaaa gattaagtgt agatgaatct
    36901 aaaatagata taatattagg tggttcacaa aaagctattt ctgcaccagc aggacttact
    36961 atagtcggaa taagtcaaga tgcaaaaaat tgtataaaaa atagaaaaac tgatgtaata
    37021 ggtttttatt gtaatttaaa tatatgggaa ggctattatg aaaaaaaata cttcccttat
    37081 acaatgccaa taagtgacat aatggggctt gataaagcac ttgataatat attagaagaa
    37141 ggaatagaaa atgtattaaa tagacatgaa aagatagctt atagtgttag aaaatctatt
    37201 gaggaatatg gtctagaact atttttagaa gaaggatatt ctaatacagt cacagctata
    37261 aaaattcctg aaagtatagg agctttagaa cttactgatt atatgcttaa aaattataat
    37321 acacttgttg caacatcact aaatcaatat atggatacta ttttaagaat aggtcatatg
    37381 ggagagaatg caaatctaaa taaaatagaa catgtactaa atgttttgga taaaagttta
    37441 agtgcattag gattcaaagg aaatggaaat ttgttaaatt tatttaataa gtattatttt
    37501 taatagatag ggggatatag tatggaagtt acagctaagc aaaaatttac aacgaaagac
    37561 atggtggaaa cctcactatt aatagcatta gtatttattg caactaaatt tataaacata
    37621 agattaccaa tatctataaa tggtgggctt gtgcatttag gaactgcaat gttgttcata
    37681 tcagcaatag tatttggaag taaaaaaggt gctttatcag gagctattgg tatgtcactt
    37741 tttgatttga tatctgggtg gactttatgg gcaccgttta cctttatagt tagaggagtt
    37801 atgggttatt tacttggaaa gattgcatgg gcaaatggta ataatgggaa taatttttta
    37861 atcaatgtaa taggaatatg tgtttcatca atttggatgc tatttggata ttatgtaaca
    37921 gaagttatac tttatggtaa ttttatagta cctcttactt caataccagg taatttaatg
    37981 caagtattga taggtcttat aatagcgctt cctatttcta aggtattgaa aaaatgtata
    38041 aaataaatat aaagtgaact aaaatttatg atagcataaa ttatactgta taatattagg
    38101 atggagtaca taattaaaaa attttagtat agaggttagt tcacaatgtt atcagttaca
    38161 agatattcaa tcaaaaatgt tcgtttaaag ccgtgggtaa agtttatttg gtattttgag
    38221 acaaaatcaa atatactgtt aaataataag ttacttccga cagacagtat agatattatt
    38281 ctcaatcttt ctgatgttat ggaatataaa attgaaaatc aagactatat tgctagtaat
    38341 attcatttta atggaataag ggataaacat ggatttatta ttcaacatgg aaatatacgt
    38401 gttataggga tttcttttta tccatttggt ctctatccat ttttaaagat accaatttca
    38461 gagtttaaca gacaaattgt tgacctagaa gcagtatcaa aactatttgc aaaaaaatta
    38521 gaagagtcat taaatccaac aaaagcaata gaaaaagttg ttttatgttt agaagaagta
    38581 ttattatcca tattagaaga agatttaatt tcaaataaat atgtaaagct tctaaattca
    38641 tttatatatg ttaatagata tagcaatata aaagcatttt gtgatgatac aaatataaat
    38701 attaaaacat tggaaagaat ctgtttaaaa tatacagggt atacacctaa gattttaaag
    38761 agaatttaca gatttaaaat ggcaagcaat caacttattt ataattataa agatgatgaa
    38821 ctttttgatt ttatttatga aaatgagtac tatgaccaag cacactttat aaaagaattt
    38881 aaaaaatttt cagggacgtc accaattaaa tttagtggtg agaataagac gattaaagaa
    38941 aatataaagt acagctattt ataattatac tgtcgatttt ttacaatacc tccaatttat
    39001 tttttgatat gatataggaa aataaattgg aggttttaca tatgaaaatt attacagaaa
    39061 tagttgagtt taatgtggaa gaaaatttat caaaagaaga gtttatagaa attgtaaatg
    39121 acttagaatt aaaatttcat tctattcaat ctggttttat tgatactgag cttttatatg
    39181 atgaaaagtc tcaaaagtgg attatgatac aacattggga atctatagaa gacctcaaga
    39241 gtgtttctag taaaatgttt aaagattctt caactgagaa atttagaaaa gctttaaaga
    39301 atcaaactgt taagatgaat ataataccac aaattaaaag ttggaaatta tagaactgaa
    39361 tgtaaaagat agttaaatag tatcaaacta aattagccaa ataacatttt gtatttaaat
    39421 tgttatttgg cttttagtgt ttattttaat atgagtgtta tatactagaa aaaccaacaa
    39481 actgttagag tgtggattat cataatgtca tattcgggag ctaaaaggct taatactgat
    39541 ttgccaattg gtcaatccat gcatgtccat ctggatatgc tgttattaaa gcatttacaa
    39601 gccaatccct ttgctcttgt gataatagtg ggactgttac atcgaagtct ttttgataac
    39661 caagcctatc tatgtatatt gatttgcaaa acaaaaccac ttcgggagcc atatatggga
    39721 tattatctga tgttatcagt ctggccttat ccatatctct tataattctt tgattttggg
    39781 aaaaaagaaa ttttccatct ttctgtggat tatatatcaa ttcaataaaa tcgaactgta
    39841 actgttcgtc acactgtata ttcattttat agttatcatc ctcgactttt tttaattcaa
    39901 taaatgagca atcaggctta atcgcaaaaa cacaaatttg ctctatatgt tgcttgttaa
    39961 ggtcgataat aggtcgtaat aaataattac ccaagggctc atatatatcc catccttgat
    40021 taagcatata ctgaatgctc ttttctttat ctgtgtcaga aatccatatg tcaatatcac
    40081 tgtgtgggcg aatatgcata ttcgcaaata attctagggc atatccacca catattgaat
    40141 atgaaaagcc acaatcatta aaaaagttta cacaatcttt aattagtttg ttcatagcaa
    40201 cctccttttg catgtactga aataaattaa attttttaaa ataccaacta aatgtttgtt
    40261 ttgtgtaaat ataaaactgc cgatattaag gcagttttat atttactatt tagaaaattt
    40321 attaacaaat ttttgatatt cacacattat cttatcattc ataaaagaaa tacccataaa
    40381 agcagcttgc tcaccaaaca aagtaaaacg ttctatcatc tgttgtggat tacctggata
    40441 aactgttgga atacaccatg tgtttagaag aaaaccaaac atttgagcag caatctctgg
    40501 atttttaact tccatagaat tatcttgatt tccttcttca atcaatgctt ttatccaagg
    40561 cacaatatca tttataatct catcaaactg cgtagctaaa tacttaggat tacgcaatag
    40621 tgatgatgac attaaaaaaa tgtcttgttt attgtgctgt tcataagtat gaagcaacct
    40681 atattgtaat ttttctaatg cggtctttcc ttcatcattt tttacctcat acccataaaa
    40741 tccatcatta tacaaccgac ttgtgattgc cttaattatt tcgtcttttg atttaaagtg
    40801 atgataaata gcacctcttg aaaggccatc taaattatct acaatgtctt gaatcgttgt
    40861 ctgctcatac cctttttcta agaataactt atgtgaaata tcaagtattt tttgtatagt
    40921 attatcaggt tctttttttc ttcccaaaaa actcactcct cgaaacaatc atttggtatg
    40981 tttatcttag caaatacata atgatttgtc aatgtgtctt tttagcatgt tgaacaggta
    41041 tattgccatg ctttcattat tttatttttt tgccttatac tggactatat ttgcagtttc
    41101 ctaatatatt tggctcatat ttaaatacca ttatctaaaa gcactattgc tgtacgagag
    41161 gcaataggca ttttagaaga aggaggagtt gagctaaagt atacatcata atctcttaag
    41221 ttttcttttt ttgtgtttcc tccaaactct accatatcag tatttaatat cgttttgtag
    41281 tttgtatttt gaaactttga aatatcaact gattgtatta gattagggtg gaaattaaat
    41341 ataaataaat atttgccctt actgtacacc aaaatctttt tatcattatc catatgtata
    41401 agttcatgac tattttggat aaaaatatca ctgtattttg taagttcgag catagattta
    41461 tcaaaattta ataattgttt atactttaag ttgttatctt ctgaaaggct ccattgtctt
    41521 ctggcatact tatagctcca attgttgcct tctcgtggaa aatcaatcca ctcaggatgt
    41581 ccaaattcat ttcccataaa atttagatat ccttcacctg ctagagaaaa agttattaat
    41641 ttaattaatt taataagaga tattgctcta ttgataacat ggttatttga gtttatttcc
    41701 atattccagt acatttcctt gtcagctagc caaaaaataa ttgttttatc gccaactaat
    41761 gcttggtcat gggattcaca atatcctata tttttttctc caggtcgtct agtagtaagt
    41821 tcataccaca ttttgcccaa atcccagtct tcatcagata agttagagat agtttttatc
    41881 caaaaatcag gtactcccat tgcgagtcta tagtcaaatc caattccgcc gtcttttata
    41941 gggatacaca taccaggcat tccactcata tcttctgcaa tacttatcga attaggttta
    42001 atttctttca ttaattcatt tgcaaattgg agatatgtta tagcttcaat atctgtattc
    42061 atactaaaat atttttcata actatcaaat gaaactccaa gaccatggtt atgatataac
    42121 attgaagtta caccatcaaa cctaaagcca tcaaaatggt actcatttaa ccaaaatttt
    42181 atatttgaaa gcaaaaaatg tattacttca ggtttaccat agttaaaaag ttttgtaccc
    42241 caagcaggat gatttccctt tgaacctgag tggaaaaatt gatgttcact tccatcaaac
    42301 tcatttattc cttctaaagt attctttaca gcatgagaat gaactaaatc taataaaaca
    42361 gcaatcccca ttgaatgagc agtgtttatg aggtttttta agtcttcagg agtaccaaac
    42421 ctagatgaga ttgcataaaa gtttgaaact tggtatccaa aagatgcata atatggatgt
    42481 tccataatag ccataagctg aatggtattg tatccagctt tttttatctt aggaagtatt
    42541 ttttcagtaa attcattata agttccaata gattcgcttt cagtagacat acctatatga
    42601 cattcataga ttagtggaga agttatgttt tttaagtcaa aatcattgtc agtccagata
    42661 aatggagttt ttggttgcca tatttgtcca ttaaatccac caaaatcttt ttgaattact
    42721 ctttttatat ataatggtat acggtcaaat gtttttccat ttgcagtaac ttgtaccttt
    42781 acctctgatt tgtgaggtaa gctattttga ccaggaataa atatttccca attaccagac
    42841 ccaatttttc ttagaggatg agattttcta ttccaattat taaagtcacc aattagtgac
    42901 aaactatctg catttggagc ccattcccta tagaagaatc catcatttgt ctgatgaaat
    42961 ccatagtaaa gatgagcatt tgcaaaagaa ctaaaatctt tatagttttt caatgttttt
    43021 gatttcatat cctcataatg tttcattcgc ttttttatat cagattcaaa tggttgtagg
    43081 tatacatcaa tatctaaaat tttaaatctt ttttgacttt tcattgtatt tctcctccat
    43141 aataaatatt acatctaaaa aataatattt atctcatact atcttctctg tataattgat
    43201 atatattata taatcctaat aaaaagctta agccaccaat gattaatttc ataatttacc
    43261 tccctagtat ttatacttat atatcgtatt catcaaaatt taaaagtttg tttaaaactc
    43321 ttttaataat atcataatca aagcctttat aggaaagatg ttgagaaatt ttttgatata
    43381 ttttcttttt atcttctttc tttactcttt catacctttt ttttgctaga tacatagcat
    43441 tttcaaattc agtgtccttg tctaattcag atactgcttc attgattgta cttctattga
    43501 tacctttatt atataaattc tgcttaattc tgtttttgcc acatttattt aggttaacat
    43561 ttgtgttaac aattttttga gctaatacac tgtcatttac gagattataa cttttaagaa
    43621 aatctatgac cctctcaatt atattttcat caaattctga tgatagtttt tcttttaatt
    43681 ttttttcaga ttggtcagca tgagataaaa tatttaatgc tttattttta gcttttatat
    43741 acatttcatc atctaaaata ggttttaatt cttcttcatt tatttcattc ccttttttta
    43801 aattaaatgt ataaaccagt tctttaaata tagcaataaa aaatttatca tctacataaa
    43861 tattaactct atcatcattt cttttttgtt gttctatttt tgtaattata cccataagtc
//
LOCUS       DAYAPA010000018        43530 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.018, whole genome shotgun sequence.
ACCESSION   DAYAPA010000018 DAYAPA010000000
VERSION     DAYAPA010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 43530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43530
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.018"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            192..1409
                     /gene="ilvA"
                     /locus_tag="ACGI59_000900"
     CDS             192..1409
                     /gene="ilvA"
                     /locus_tag="ACGI59_000900"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HFG9738705.1"
                     /translation="MMNVTLTDVKEARETIKNIVKRTDLLESVKLSEKTGANVFYKCE
                     NLQKTGSFKLRGACNKIASLTDEEKANGVIASSAGNHAQGVALGAKMTGIKSTIVMPA
                     TAPLAKVSATKGYGAEVVLNGAVYDDAYAKAVEIQKETGATFLHPFNDKYVIAGQGTI
                     SLEIFEQLDNKVDTILCPVGGGGIISGVAVAAKALNPNVKIVGVQTANIPSMKESIKN
                     GKVTTAFNDTTIADGIAVKTPGDLTFEIINELVDEIVVVEETEIAESILFMMESQKIV
                     SEGAGAVCTAAILSGKYVPAKDENVVCIISGGNIDINTLYRIIGVALAKEGRRYSFST
                     IMEDKPGNFAELTRIISENGGNILSANQGKLSAGEALGKQSAEFILETIDYDHIARIK
                     KAIEEKGFKIIEL"
     gene            1504..1884
                     /locus_tag="ACGI59_000901"
     CDS             1504..1884
                     /locus_tag="ACGI59_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFG9738706.1"
                     /translation="MKHEVIHTNDAPAALGPYSQAIKAGNLLFVSGQVPLVPETMEVV
                     EGDVQAQTAQSLKNLKAILAESGADFSNVVKTTVFIKDMNEFGAINEVYAEYFGENKP
                     ARACVEVARLPKDVKVEIEVIAVL"
     gene            2168..2650
                     /locus_tag="ACGI59_000902"
     CDS             2168..2650
                     /locus_tag="ACGI59_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFG9738707.1"
                     /translation="MLNFFKKNKSYKLHAVVSGNSINIEKVNDSVFSKKLMGDGVAII
                     PNSNVVVAPCNGKVTVLTESKHAFGMVSDEGVEILVHIGIDTVSLQGEGFKNEVSQGD
                     TVKKGSPIISFEREKINSQGIDCTTIIIVLNHSEFSEINCMVENEVVAGQDTVIEIMK
                     "
     gene            2671..3486
                     /locus_tag="ACGI59_000903"
     CDS             2671..3486
                     /locus_tag="ACGI59_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFG9738708.1"
                     /translation="MRVVKIFNNNALSTVTNDRKEAILLGLGIGFNKRPGDKVNEDKI
                     EKIYYVQDHMQTKFLELLKNVSPEVMDASQQIISLIPGNEGKFNNKGILSLVEHISFA
                     IDRMKNNVFLPNLMLLDIKMMYSKEFELGVKALEIIYRVCHIHLPEDEAGYIALHFVN
                     LQSNDNLAYDTLKFVKGSIDLIKECYGLELDESSLSTLRFRTHLKFLAQRIFKNEICQ
                     DDKMIEMYDYLINNHPKNKEYLEKLNVYIEKEFRYKLDKPEKIYLLLHLTKIL"
     gene            3537..5087
                     /locus_tag="ACGI59_000904"
     CDS             3537..5087
                     /locus_tag="ACGI59_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFG9738709.1"
                     /translation="MKEKLLGGLETFAKAMVQPLMYLSSAGILMVVGVLLTNDMLLGV
                     LPFFKWEPIQIFGSLIYNCIMAIINNLAVIFAIGIPAALAKKEKHKAALIGFMSYIIY
                     LTASNTMLTSLGQLAKESKMLGLIGTGQATILGIQVVDTSVFGGIILGCIAGYVFNRT
                     SNKTFKGALQIYSGANFSFICMFFVSILIGVVSNFIWPLAQQGIASLANVIKDTGTFG
                     LFLYGFLERILVPTGLHHLIYTPFQFSDLGGVLKVGADTVSGAYAIVMTEMNLPVERF
                     SDSIYYMATGFTKMFGYIGIGAAFIYTAYKQNKKKVKATIIPLIITAVLASVTEPLDF
                     MFVFCAPFLFFVHAVIAGLFIALLKIFDVTALCGGNLLVSIIMNTTVGVEKTHWPVMM
                     ILGLAQIIVYFVVFSFLIKKFNYKTPGREDESTATGEEQSKQLNLDAGIENIIDGLGG
                     KENINTVENCITRLRVNVKDESKINEDIINLTPNSGIVRKGKDIQIIFGLHVHEVRRA
                     VEDFLEAY"
     gene            5184..6536
                     /locus_tag="ACGI59_000905"
     CDS             5184..6536
                     /locus_tag="ACGI59_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HFG9738710.1"
                     /translation="MIITIVGGGSTFTPGIVKSIALREKELELEEIRLFDIDKERQDK
                     VAVVVNWILKEDVKTDVKLTVTNCEKEAYTDATFIFAQMRVGKYEMREQDEKIPLKYG
                     CVGQETCGCGGMAYGLRTIQPMIKVIDDVEKYAKKDYWILNYSNPAAIVSEACRKLRP
                     NARIINICDMPIAIIDMIAGSLNINDVHNIRYDYFGLNHFGWFTSIDYKHRDLMEEIK
                     EYIKENKILLPKSYIENLNKSRSTGNRHAVSSWIHVWESVYTMLESFPKYLPNTYLTY
                     YLLSKETVEHSNINRTRANEVMESREKDLFEGVRHYLETGEISEKAFYAGSHGDWISD
                     LAVSIKNDTRSRFLVITENRGAIPNMPYDAMVEIPAYIGKDGPEVIARGPIPLFQQGL
                     MMQQLNSEKLIVEAYIENSYEKALQAFTLNKTIPSMNVAKEILDEMIEVNKEYWPELR
                     "
     gene            6667..7425
                     /locus_tag="ACGI59_000906"
     CDS             6667..7425
                     /locus_tag="ACGI59_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFG9738711.1"
                     /translation="MNIINKIFWEEDRIGMITNNLEADTHSHCMLQLFLGIEDSIEIT
                     VNEKLVKCNCIIVDKNISHSFSARKKVYYSAIIEPTSIYAEQLTSKMNDFGYWICDND
                     GLEKLRQQGTFLIDNSSKEQYLRFMEMLNNYLNIPITLKHYDDRITELLNLLRTCNCD
                     NHTISSFADKVSLSASRLSHLFKEQIGIPLKSYILFHQMECAFRELLSGKNVTEASMT
                     AGFDTPSHFAGTVKRMMGMPVSLSLKDSEFLKVY"
     gene            7472..8191
                     /locus_tag="ACGI59_000907"
     CDS             7472..8191
                     /locus_tag="ACGI59_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HFG9738712.1"
                     /translation="MNKEYLEETKMLNYNEPQLKLLVSSKNWLELDDFHKIKSIYEFV
                     QNDILFGYNASDMLSATQVLNDGMGQCNTKATLLMALLRAVNIPCRLHAFDVTKDFQR
                     GATSKLISLLAPKYILHTWVEVFYQDRWIALEGVITDKKYLEAIQKKFFNHGGTFKKY
                     AIATNDLKNTSIDWDGKDTFIQKEAIVYDYGIFPSPDVFFSTHSQHMSKLKNFIYVHL
                     IRKIMTKNVCKARNNYIDKNE"
     gene            complement(8315..9715)
                     /locus_tag="ACGI59_000908"
     CDS             complement(8315..9715)
                     /locus_tag="ACGI59_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFG9738713.1"
                     /translation="METQSNNKKGNLIIAIVMTGAFISSLSQTLLSTALPNIMSDFKI
                     TADIGQWLTTIYLLIAGIIVPTTAYLINRFSTRKLFITSMSIFSIGCIIALFSNNFST
                     MLIARVLQAMGSGSLMPLLQVIILYLCPEEKRGAAMSLVGITVGFAPAIGPTLSGWLV
                     DSFGWHSLFLFLSPIAILDVILSFILLRNVGETQKLKLDIPSIVLSSLGFGGLLIGFT
                     NQGNYGWTNIATYLPILIGIMSLILFTLRQLKSKEPFLELRVFKNKPFLISTILIMIV
                     YASMMSATLMIPLYVQSVRGFSALSSGSLMLPGAILMVVLNPIAGRHLDKYGPHALSI
                     LGTGCLFLGTLSFAFLGRDTSLIHVSLMYCIRMIGISMVLMPLTTWGIKTLDRELISH
                     ATAINNTLRQISGAIGSAILITIMTSATKKAHMSSNMLSNIHGIDVAFSIAATLAFTG
                     LIVSICFIKRHQIIRS"
     gene            complement(9719..10609)
                     /locus_tag="ACGI59_000909"
     CDS             complement(9719..10609)
                     /locus_tag="ACGI59_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFG9738714.1"
                     /translation="MTEKQRLIITIAQKIFDQKGFQNTSISDVVKECKMSKATFYKHF
                     ETKESFICEIINYYDEKFLEIIHSINENNDIPSSEKLNRKIIAVWKNIFSRTTINTYI
                     RENFSEEQRKATSKLQTKSRANLLNEYKLSLFDNYGDKIEKIIFDLVFLLDALIHQFI
                     YIIHVQKREINVYFIAKFTIQILDLVVENMDNLNPLIEKSMFLHKQEDEVYFSLHNKS
                     LFFKTIQDIEELIKTDTSLLENPKLLEAIKKLYVEGKNQQYDSLIMDAMIAYLEKEDT
                     LRPKVLLLNSIKNQLKKETL"
     gene            10888..11535
                     /locus_tag="ACGI59_000910"
     CDS             10888..11535
                     /locus_tag="ACGI59_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFG9738715.1"
                     /translation="MKKKICVAIIFALFIVFVEMFIDSSSEGSKIMTAELSKYRKGVN
                     VLSINNIIVANKKYSLPKDYSPQESSEARDAFYKMNKDAQKSGLNLKAFSTYRSYEYQ
                     DRLFKSYVKEHGEKEANRFSAKPGESEHQTGLAFDIGGDDQSCWANKKFNNTKEAKWL
                     YENAYKYGFILRYPEGKENITGYMYESWHYRYVGTEHSKNFAMNNLTLEEYLHIN"
     gene            11860..14499
                     /locus_tag="ACGI59_000911"
     CDS             11860..14499
                     /locus_tag="ACGI59_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFG9738716.1"
                     /translation="MSYKSTYQEVIKNLDSSLSGLSKERAEELLEKNGANELKEADKV
                     PTYKLFLESFKDPLVIILLIAALVQIFLGETVESIIIFAVIIINSVLGVVQTKKAESS
                     LESLKNLSAPNAKVIRDNKKMTIPAKNLVVGDIVFLEAGDYIPADGRLIEAQSLKVVE
                     GMLTGESEPVLKHIEKISEDVSLGDQKNMVFSGAVVVYGRGSFVVTGTGMNTEMGKIA
                     GLLESAENKLTPLQQKIEDFSKKLGVGIVILALLIFIIQVARNYFMAGNGEMTQIILD
                     SFMFSVAVAVAAIPEALSSIVTIVLAVGTNSMAKKQAIIRKLPAVETLGSTSVICTDK
                     TGTLTQNKMTVVDYYMYGTNNDELDKEKVNYSYHAKLLTMVSTLCNDSHITSDGKEVG
                     DPTEVALINYSSKNDLDYDKLRSKYIRINEIPFDSDRKLMSTVNSIYGDYIMFTKGAP
                     DIVFSRCKYALKNGSKVDITDEIIEEYKHKNEEFSNRALRVLAFAIKDVPEEDFIPSI
                     DDEHDMTLVGIMAMIDPPREEVMDAVKEAKSAGIKTVMITGDHKTTAAAIAKEIGIME
                     EGDLALTGKELDALSEEQLYEKLENISVYARVSPENKIRIVKAWQHKNNITAMTGDGV
                     NDAPALKQADIGIGMGSGTDVAKDASAMVLVDDNFASIVNAVEVGRTVYSNIIKSITY
                     LFAGNLGAIVSIVFAVFAGWSNPFTALQLLFINLVNDSLPAIALGFEKSEKNIMNKPP
                     RHPDESILAGGTLKAVAIRGCIIGVVTIIAQYIGLQVSPELGVAMAFSTLILARIMQT
                     LPARSNNQTVIKLGFFSNKYVIGAVLVCLGLYSLTLIPVLRGIFSIPTTFGLYQLGIC
                     LGLALISSILMELNKLINRSEVK"
     gene            complement(14557..15660)
                     /locus_tag="ACGI59_000912"
     CDS             complement(14557..15660)
                     /locus_tag="ACGI59_000912"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFG9738717.1"
                     /translation="MKLKINKAIEMQLKKSYAIESGHSEDVFEIDCGEGINTVSYSNK
                     AVEAFNALKFDMIRGYPHSIALKDNIVDYWKDFIALDTDRICLADGSIHVIYLLNRLF
                     IEKGDKVLGYSPQFSEYETDIKMHGATYDYVLLKKEDNFKFNEKEFIEKINPEYKVIY
                     IDNPNNPTGQIIPLSSIENIVREAAKYDIAVMVDEAYGEYMPKENSAVKLLNNYDNVI
                     ALKTFSKGFGLAGLRAGYAVLPEQLVSPIKKISTPYEVSEISRSIAANLLDDVQFIEE
                     LKEKTKDIKNQLLIPWKNLNIAETSDTVSIMTVEHKNKDIDLQQEFAKLKIRVISGSD
                     FTGLDKNFIRFRMPEEKELPEVIKAFQIIDNIE"
     gene            16020..16841
                     /locus_tag="ACGI59_000913"
     CDS             16020..16841
                     /locus_tag="ACGI59_000913"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFG9738718.1"
                     /translation="MGYKLIVTDMDGTLLGNNHKVTDENKTALQKVIKSGINVTLATG
                     RAFDSAKCNVDFLKEDMPIIACNGSLIREQNGNIIYSNKIDTRTCLNILDVLDKYDIY
                     YQCNSIDSMLSKKIEGREDRLSVFLGSETEVIVKDDLREEIFKKDILKFVIIEEKNPS
                     ILDEIRKELRKVQGIKITSSWPNNIEVMNEGVDKGNAVKILAEKMNIDREDIIAFGDN
                     YNDIEMIKFAGLGVAMGNAEELIKQEADYVTDTNQDSGVAKAIYKFLELKESLSS"
     misc_binding    16920..17131
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            17218..18534
                     /gene="argH"
                     /locus_tag="ACGI59_000914"
     CDS             17218..18534
                     /gene="argH"
                     /locus_tag="ACGI59_000914"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HFG9738719.1"
                     /translation="MKLWGGRFRKAENQLMEEFNKSFGYDCVLYKKDIEGSVAHVHMQ
                     VKCGLLTEEEGKSITEGLKGILEDVENGKLVLDGEYEDIHSFTEINLIQRIGDVGKKL
                     HTARSRNDQVAVDMRLYAKEKANDLVDLISEFKATIKDVADKNPVMMPGYTHLQRAQV
                     VTFKHHLMAYYSMFDRDEKRIKNAIEILDESPLGCGALAGTTHDIDRSITCEQLGFKK
                     VVDNFMDGVSDRDYLLELMSDFSIIMMHLSRLSEELILWSSQEFGFVEIDDLYTTGSS
                     IMPQKKNPDGAELIRGKTGRVYGNLFGLFTVMKGIPLAYNKDMQEDKEGFFDSVHTLE
                     MCIQIMDRMIATLKVNEDKMKQAVKNGFLNATEVADYLVKNNVAFRDAHGIVGSIVIY
                     CEDNKKAIEDLTLEELQKFSDAFKEDIYDFIDYESILNKGIKKNLK"
     gene            18783..19121
                     /locus_tag="ACGI59_000915"
     CDS             18783..19121
                     /locus_tag="ACGI59_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2325 domain-containing protein"
                     /protein_id="HFG9738720.1"
                     /translation="MNLLVIGGHERMEKDYMVMAKKKGYKTKVYTTMSSKLNNSIGKP
                     DAVVILTSTVSHKMSRTVESQAKKQDILIVRHKNSSKVAFNECLDMVDECLGNCSKCK
                     LKDKQKQKNI"
     gene            complement(19248..20537)
                     /locus_tag="ACGI59_000916"
     CDS             complement(19248..20537)
                     /locus_tag="ACGI59_000916"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFG9738721.1"
                     /translation="MKRNLSLLLICLLIFTSFLGSSNISFADNEPAIVAKHAVLMDYE
                     SGKILYNKDGNSKLYPASTTKVWTACLVLKEVKDLNQVIEIKDLPQIDGSSMYLKNGE
                     SFTVKQLLDALLVHSANDAAFVLARYVGGGNVQKFIDLMNSEAKKIGATNTHFNNPHG
                     LPDPNHYTTAHDMALIAREAMNNDTFRQIVKTKSLKFEATKAYPYERYFVNTNKFLTS
                     HDKITYKGQPINIKYDIVDGIKTGYTDAAGKCLLSSAVKDGRRVIVAVFNSTNADLYL
                     DSRILIDYGFDNFKCATIVDKEKYTDTKKVLFTKQHELIYEPKNSYRIFLEKDESQGN
                     YDTKTELNKIDLPIKKGAKVGTLNVYNNGKLENSIDLIAKNNLDSSLPFLTENNVLMT
                     FVKIIAGILVLLVLFIIASNIKKKKRKKARSKKNMKK"
     gene            20617..21321
                     /locus_tag="ACGI59_000917"
     CDS             20617..21321
                     /locus_tag="ACGI59_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFG9738722.1"
                     /translation="MKKKVKFISFFVLFILIISSIIVYKSLIFKDDIYVVSKKNLPNE
                     KTHVVENEIKGEDNNHSKVTKDIDKNFQNNKITIYISGAVNRPGIVTIESDKRLYDAV
                     ELLGGTTKEADLNGVNLSVRLEDEQHYIIPKIGEATSVTSNDDSNKLNQKNESRGEPK
                     NKSNKNISNESKVNINVATIEELDSLPGVGEATANKILQHREENGQFSSIEEIKNVNG
                     IGDKKYENIKDLICVD"
     gene            21413..22459
                     /gene="selD"
                     /locus_tag="ACGI59_000918"
     CDS             21413..22459
                     /gene="selD"
                     /locus_tag="ACGI59_000918"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:21461..21463,aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HFG9738723.1"
                     /translation="MKKEQKKLTEMTTAGGUAAKIGPEVLASVLSQLPKNDNIENLLV
                     GLDTADDAAVYKLNDDMALIQTLDFFTPMVDDPYVFGQIAASNSLSDVYAMGGKPLVA
                     MNIVCFPSCHDMDVLAEILKGGFDKVKESGALLVGGHTVDDKEPKYGLSVSGIVSPNK
                     VLSNATAKPGDKLIITKPIGVGVLNTAMKEGMVEQHIADKVIEIMIHLNKYAAMSFEK
                     FDVNSVTDITGFGLLGHTLEMAKASEVSIEIESKHVPIIEGAIEMAQMGIIPAGMYKN
                     MHYVSKDVEVVGNIEVAVQDILYDPQTSGGLLISVKEELAEELVKDMKLNGAIEAKII
                     GSVVPKGEKYITVL"
     gene            22510..23889
                     /gene="selA"
                     /locus_tag="ACGI59_000919"
     CDS             22510..23889
                     /gene="selA"
                     /locus_tag="ACGI59_000919"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HFG9738724.1"
                     /translation="MLPSVDEVLSDNRIVEIINEYPRSLVLESVREVIDLKRQFILRL
                     KEDASNSVTIEFGEIIESAIERVKLNYSLSLKKVINATGTVIHTNLGRSLLSEDIKDE
                     LWCAASRYSNLEYDLDNGERGSRYSHLTSTIKRLTGAEDVLVVNNNAAAVLLVLSTMA
                     KGGEAIVSRGELVEVGGSFRIPSIMALSGAELVEVGSTNKTHLKDYKEAITEDTNVLM
                     KVHTSNYRIMGFTESVSIEELVNLGKKYKLPVIEDLGSGVFIDLSKYGLSYEPTVLDS
                     IRQGADVVTFSGDKMLGGPQAGIIVGKKEYIEKMKKNQLTRALRVDKLTICALEATLR
                     MYLDETKAIENIPTLKMLTYKIEELEVKANKLFEKITALNLNANINIEDGFSQVGGGS
                     MPLETISTKVISITPEHMNVSSLEKKLRLSEAHIIARVYDNKYVLDVRTIFDDEFDVI
                     VEELRKAFN"
     gene            24003..25886
                     /gene="selB"
                     /locus_tag="ACGI59_000920"
     CDS             24003..25886
                     /gene="selB"
                     /locus_tag="ACGI59_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HFG9738725.1"
                     /translation="MKNVVIGTAGHIDHGKTTLIKALTGRETDTLDEEKKRGISINLG
                     FTYFDLPNNRRAGIVDVPGHEKFIKNMLAGASGIDIVLLVVAADEGVMPQTVEHLDIL
                     SFLNIKNGIVVLTKSDTVDDEFRELVKEDICEKIKGTFLEDAEIIEVDSISKRGIDTL
                     IEKIDKMSDDIEDKNENSPARLNIDRVFSIKGFGTVITGTLIEGKISIEDDLVIYPQV
                     LKTKIRSIQVHGENKDTAYAGQRTAINISNVKVDDIKRGDVLAAPNSLEESMMLDVKL
                     SLVNHLDKGLKHWDRLRLYHGTREILCRAVPLDKELIESGESGYVQLRLEESIVSKKG
                     DTFVVRRYSPMETIGGGVIIDPSPKKHKKFDEKVIEALKIKEKGELKDIIEEYLKRNL
                     KNYPNIKEIMSYSGAHEEDVKRALETLISEDKVFIIGNMYMHINQYNKLKENTIKLLS
                     EYHKKYRLRKGILKEEVRSKIESNFKTREMDILLEKLSTENAIKIENNIVSLLDFEVI
                     LNDKQKEIAKKIEKRLKSCGVSSILTIDEVSEGNHNYAEVLESMIGNKVEKLDDLYIM
                     DKDIYENAKNILINYIKENKEITLGEYRDLIDSSRKNCMIILENFDRNKITKRVENKR
                     ILF"
     gene            25991..28729
                     /locus_tag="ACGI59_000921"
     CDS             25991..28729
                     /locus_tag="ACGI59_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation-associated two-component system
                     sensor histidine kinase"
                     /protein_id="HFG9738726.1"
                     /translation="MKNIIPDVKPDIKEREDMSLLFIIMGSVLLLFIIKDIEKYETLL
                     HAIYTIFLVATGVIIFNTTKFKVDSFSTFLGLIFIATGVLECIYLFNSLGIKTKSIME
                     INITISAITDLFPILGVYLSFKFVKDNKQIYSSVILFIATATLTITSLFIISGICDYL
                     GNGKSQYALGVIISIFIIIISIISGIELNSSSNKGKWEYDEKKIFNRIIVIIILSRVP
                     NLLHIVIDNRNVERILSQIIINVALYYLYNYIVSKNIKKTALELHDTNEELTKKTESL
                     KEKNKKLIYETEKIEELKGILMKRESRLQSTLDVAVNSIVVFSKDGEITYANKSFRNT
                     FGDYKEQDKLTDKIKNFNDLIESIHYVFVHEKNAEKLISTSDNKVYQAIFAPLIIASQ
                     TEGVLCVLEDKTKKKEYERKLIEANKRSKDFLESIGDGIVVLEGNNKIYVNNACREIF
                     KNNLESIDFSLVCKSEESIEKRYVIDGEVKYVEMSFSQYTNAGTNKTIIVIRDTTSRK
                     ISQIKLEESQSSYSRFIDILPDGICLLKEDLSINYANKSFLDMLAFTNIDDIKDSNIK
                     LLMNANTEEKMKFTDNMAKVLNENRSILLLEHELINSYDDIVEVELSALPFAIYNTRY
                     IMLIIKDLVHKKYSEQAEKELLERFKTDKIKTEFFANMSHELKTPLNVISSSNQLVDS
                     FYRNEKIKDYNNNIKSHVDLVRQSSYRLQRLINNIIDLTKMESGFYTLKLAKYNIVSV
                     IEDLFMNIEEYALRKDIKILFDTDLEEINVYIDKVEIERIMLNLLSNCIKFTDNGGWI
                     YVSIHYKIDKVIISVKDTGVGIPQDKLELIFEEFSQVDKTLSRNTEGSGIGLAIVKNL
                     VSLHGGDIEVVSEVNKGTEFLISLPMKGFSSEHYTEDKRIYNIQEKIKIEFSDIYY"
     gene            complement(28848..29792)
                     /gene="manA"
                     /locus_tag="ACGI59_000922"
     CDS             complement(28848..29792)
                     /gene="manA"
                     /locus_tag="ACGI59_000922"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HFG9738727.1"
                     /translation="MEPLFLKPIFMDRIWGGTALKDKFNYEIDSPTTGECWAISSHKN
                     GDCLIENGKYKGKKLSELWNKNRELFGNTPGDKFPLLTKILDANDNLSVQVHPNDEYA
                     KKNENGELGKTECWYVIDCSDDAEIIIGHNAKSHKEFVNMVNNNEWDKLLRKVNIKKG
                     DFFYVPSGTIHAICKGTLILETQQNSDTTYRVYDYDRTDNSGNKRELHVQKSIDVTNV
                     PHINFDTDYKIVSTSDFKCTTFVSNEFFSVYKLDVFGKCNFTHNTPFSLYSVLDGNGK
                     LIHNSVEYNLKKGMHFILPNDFGDFSFEGNLEIICSHI"
     gene            29965..31326
                     /locus_tag="ACGI59_000923"
     CDS             29965..31326
                     /locus_tag="ACGI59_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFG9738728.1"
                     /translation="MELFKAESKKYKKEFLKLLEQWVSIPSFYDRKTVSKDMPFGKGV
                     YDALNWFENLGRENNFKVKNIDNHAVQIEYGNGKEYVDIFGHCDVVNPGEGWDSEPFK
                     LNIIGDKLVARGVSDNKGPMIVNFLALKMIKDLDINLKRKVRLIAGGNEESGFKCIKH
                     YYSKEPYGVCGFTPDAKFPVLNGEKGGAIIKLISNIDDKSLYISGGIEFNTIPDKVYI
                     KNVEKLGKDNICFDINNISINYDNGNYIVYGKGGHSSKPEKSINPILATIKLLSENID
                     EKWTKDLYKLINQDNINGNLFGLNIEGKCGILSMVPTIINIVDGKLEVVLSVRYPEIL
                     TIEDIIKKFNLYMEQNNINKFELIGENLKQANYIDRNSKLVRSLHDIYIKYSGDLKND
                     VRVTSAGSYASEMNNSVIFGCEFPDGSFGNVHSANEFASLDRFITAIGIYAEAIITLC
                     NKI"
     gene            31414..33303
                     /locus_tag="ACGI59_000924"
     CDS             31414..33303
                     /locus_tag="ACGI59_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFG9738729.1"
                     /translation="MITKKHAIIVKSLSNKDGYMTSNELAIKLDVSTKTIKRYIADLN
                     SVLSKYDLVIASSRGIGYKLSGSKNNIARAVKEANKYIDGFLDDSEESRMSNIICMLI
                     NRNYMSIEAMAEELNLSIAAINKLSSKLKRKLEKYDLVIKSKPYYGSYIHGEEINIRQ
                     LITDYAIKLDEKSKVKVFLDDISENDIHCIESILEKHLREKDTIISDKDFNLLLSKII
                     VSVFRSKRGHSNNINLMDTSYRFHNYYFIENLMKEISDKIGFKLIEDEVIYISNYSGV
                     IAYKGTQGRKNTSEIEERISSVISSALQDIFLISGSDYTKDDEFMVAISDHIKRFLNR
                     ARANVKSNNPLLHQIKEKFPIAFNLAVFLSNKLETEFNLKLDEDELGYIAIHFAASNE
                     RMKKNTSKKICIVCHYGIGTGQLISEKLKQNISDLSVVGVYPVRYLDMAISQDVDLIV
                     STVELKGYEKPVLYIENIFDDSLIENVNKAFYEKEERRKIISNMFDEKAFFSIKANTK
                     EEVIISLSNKLKERDFIEESSIKSIIDRENISSTEIGNLVAIPHTIVKGDKKSIIGVG
                     ILENPIIWDKQEVQLVFMVFFNTKEKHNFSIFKYLYNFIKDEGGVRGIIKICDFNKLM
                     ALIGN"
     gene            33318..33773
                     /locus_tag="ACGI59_000925"
     CDS             33318..33773
                     /locus_tag="ACGI59_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFG9738730.1"
                     /translation="MGTNIFNKEYVFLNVDAKSKVEVLKFISKKAKDLNLAEDESLVY
                     EGLMAREEQFTTNLGESIAIPHTKNDAIENPAVVVLKFNEDVVWNEGEDKVKLAISLL
                     MPGKSKENIHLKLLSSLSRKLINKEFKDSLLKSDNVEEISNLINEALGL"
     gene            33832..34149
                     /locus_tag="ACGI59_000926"
     CDS             33832..34149
                     /locus_tag="ACGI59_000926"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: protein motif:HMM:NF014368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFG9738731.1"
                     /translation="MNKKLVALCACPMGLAHTFMAAEAIEQAAKALGYEAKVETQGAD
                     GVQNELTRDDILGATMIIHAVAITPEGMERFDGCEVYEVELQEAIKNAEGVIKEIEED
                     LGI"
     gene            34174..35436
                     /locus_tag="ACGI59_000927"
     CDS             34174..35436
                     /locus_tag="ACGI59_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFG9738732.1"
                     /translation="MAIKKKVINSSGASTTGGNNVGKSTPSSPTQSKGNGKWKEISKH
                     IMTGISYMIPVLVMGGLIGALSQLIPYAILGLDPSVGIVDAMNSGEFTGFKLSLLNIA
                     QLMSNFGFTLFGFAIPLFAAFCANSIGGKTALIAGFIGGYIANKPVGVPQFVDGQWTE
                     VVPVASGFLGAILIAFIIGYFVKWLNKSIKVSHNWLAFKTTFLIPLIASLACMVLMIF
                     IITPFGGLINESMKNFLTAAGAAGEYVYATALAAATAFDLGGPINKAAGFVALGLTTE
                     NVLPITARTIAIVVPPIGLGLTTLLDKRLVGRRVYDRQFYQAGKTSIFLSFMGISEGA
                     IPFALERPGFVIPLNIVGSVIGAITGIILGAIQWFPESAIWAWPLVDNLFGYIIGIAV
                     GAIFIAVGNIFYRNKLIKDGKLVVDYID"
     gene            35541..36605
                     /locus_tag="ACGI59_000928"
     CDS             35541..36605
                     /locus_tag="ACGI59_000928"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HFG9738733.1"
                     /translation="MDKIEKVREYFREYDIDGFLINSPTNKFYVGNLFSSSGYVFITK
                     ESQYIIVDFRYFEEIKRKSSLFNVVLMDKTRTHFDIINDICREQNIKEIGFEGNEVSF
                     DLYRSMSNKLSATLKSVDLSTLRETKNEDEIKYIKKACEIVDATFYHIVDFIKVGMTE
                     KQVENEIVRIIKDLGGQKESFDTIVASGLRGALPHGKASEKVIEYGDFVTFDFGAKYN
                     NYCSDITRTICMGTINKELEEIYNIVRKANEECIRVLRPGMTTGEIDKVARDIIGSYG
                     YAGNFGHNLGHGVGIMVHEYPALAPESNEVLKEGMIVTIEPGIYVPSLGGVRIEDDVL
                     ITQDGCMRLTTSTKDLIVVS"
     gene            36659..37837
                     /locus_tag="ACGI59_000929"
     CDS             36659..37837
                     /locus_tag="ACGI59_000929"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFG9738734.1"
                     /translation="MYNFDDKPDRVSEKCRKWDLNIIRDKFGDIREDFIPMWIADMDF
                     KIPTEIENKFIEAVRRGVFGYTYCYDEFYDSVIGWQKNMHEVNVEKEWITLTYGTVST
                     LHYVVQAFCKKGDSIILNTPVYDPFESSAKKQGVNVICNTLDVVNDRYYINFDKLEVQ
                     IKENKPKLMMFCTPHNPSGRIWTIEEMTRVATICKENNVILVADEVHAEHIHYGTFNS
                     ILKIGKELLENVILLTSPNKGFNLGGLKTSYSIVINKDIRDKFRNKLKQNSITSPNVF
                     GIIGLITAYNECHEWLSGVNEYIKSNYELLETWVNKYNKIKLMKMESSYLAWMDISGL
                     GISASEFTDKLAIDTGVLLEDGSHFVKDGEKYVRINLGTQKENVIEALNRMDLFLKSL
                     "
     gene            complement(38028..39086)
                     /locus_tag="ACGI59_000930"
     CDS             complement(38028..39086)
                     /locus_tag="ACGI59_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFG9738735.1"
                     /translation="MNKKKITFIFVILILALILFSFFRSSSQNTKYSKTSYYLGTVNE
                     VTVFNVKESKSDKILNECDSILRYIDNKMSTHIPGSDVSKINDNAGKKFVRVSDDTFF
                     VVKEAIQYSKLSDGYFDITIGPLSNLWAIGTDKAKVPSDSEIQKLLPLIDYKNIILDE
                     KNKSIKLTKENMKIDLGAIAKGYAADKIVAYLKSEDVKKGLVNLGGNIFTLENGKNDK
                     PFKIGIQDPTSKNGESIGNIETTNKSVVTSGIYERFIEKNGKIYHHMLNPFTGYPFEN
                     NLSSVTIISDKSINCDALSTSTFGLGLEKGMNLINKIDNVDAIFITKDKKVYLTKGIK
                     DNFKLTDKSFSIEKLNKQ"
     gene            39153..39524
                     /locus_tag="ACGI59_000931"
     CDS             39153..39524
                     /locus_tag="ACGI59_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HFG9738736.1"
                     /translation="MKKKDILLILGILVVITACYGIINVINSKNAGNIEIYVDNKLYK
                     TVSINAKEEFKIENRGGYNIVKIHDKGVEIVDASCPDKVCVHTGFINKPSQSIVCIPN
                     RVSIKINTNEKNDNQEDIISK"
     gene            39561..40235
                     /locus_tag="ACGI59_000932"
     CDS             39561..40235
                     /locus_tag="ACGI59_000932"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFG9738737.1"
                     /translation="MVNLGNDWDELLKEEFEKDYYLNLRKFLIEEYKTRQIFPNMHNI
                     YEALKHTSYKDTKVLILGQDPYHGDNQAHGLAFSVQPQVKTPPSLLNMYKELKDDLGC
                     FIPNNGYLMPWADQGVLLLNTALTVRAHEANSHKNKGWEIFTDRVISILSEREDPVIF
                     VLWGSNARKKVELIDTSKHYILEAPHPSPLSASKGFFGCKHFSKINEILKKLGKEPIN
                     WQIENI"
     gene            40484..41323
                     /locus_tag="ACGI59_000933"
     CDS             40484..41323
                     /locus_tag="ACGI59_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HFG9738738.1"
                     /translation="MNFKKLSNAKVSVMAVVLSVGILSGYSALNKQVTLVVRGEERTI
                     STFSSNVGDLLESQNISYDANDIVTLDENTKLSNGDKIEVIDVKEKTVVETKDIPFEV
                     TVVEDGSLLKGDSKVKEEGKSGKSELIYKETYHNGKKVEKEFVKKVVKAEPVNKVVSK
                     GTKVEMQVASSRGESSRRMASSSSSSSSSNNGKNMRVVATAYTGHSITATGTKPKWGT
                     IAVDPKVIPYGTKVYIPQFDMVFTAEDCGGAIKGNKIDIYMNDSKTVYNWGRKTIDIR
                     ILK"
     gene            41567..41663
                     /locus_tag="ACGI59_000934"
     tRNA            41567..41663
                     /locus_tag="ACGI59_000934"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:41601..41603,aa:Sec,seq:tca)
     gene            41813..42520
                     /locus_tag="ACGI59_000935"
     CDS             41813..42520
                     /locus_tag="ACGI59_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HFG9738739.1"
                     /translation="MENNFTTRTSFLVGDDGIEKLNNSNIIVFGVGGVGSFTVEALAR
                     AGVGNITIVDFDDVDITNINRQIPALHSTVGRYKVDVMEERILDINPNINIKKIRSLY
                     NKDTSDEILTERYDYVVDAIDMVSSKIHLIETCEKKGLKIISSMGMGNKLDPTKIVVT
                     DIHKTSTCPLAKVMRKELRDRGIKKLKVVYSTEQPIELKKKVMNGRKVTPGSVSFVPS
                     VGGLIIASVIVNELLGQ"
     gene            complement(42662..43498)
                     /locus_tag="ACGI59_000936"
     CDS             complement(42662..43498)
                     /locus_tag="ACGI59_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFG9738740.1"
                     /translation="MRNKIFLSILIIISLLIGCDKKSLLSIHMIDVGQGDSILVQTPT
                     NKNILIDGGDEDSENIIISYLRQKRIKTIDIIIATHPDSDHIGSLDNIIKKFNVNSIY
                     MPEQSTDSEAYQNLINSCTDKNLSIQHLYKNDVLNIDNNINIYVLSPSYIQEESNLNS
                     IVFKLTFNDNSFLFMGDAEEENEKEILHSFKLNNINFIKIGHHGSNSSSSLEFIKKIS
                     PDIAAISCGYKNQYGHPHREVINNLKQNHVSIYRTDRIGDIVFYSDGEIIFTKYNYEI
                     DT"
BASE COUNT        17019 a         4803 c         7529 g        14179 t
ORIGIN      
        1 aaattatcat ttaatgtaat ttaaatgaat tgaagatata tgtttattgt atgcaattgt
       61 tgtattttta ggttttttgt gattaattaa aaaattataa aaatcgttta catgttgatt
      121 tttaaaaaaa aaagatatag aattatatag aaagtaataa acaaaaattg gatttacggg
      181 agtggaatga aatgatgaat gttaccttaa cagatgtgaa agaggctaga gagaccatta
      241 agaacatagt aaaaagaact gacttacttg aaagtgttaa acttagcgaa aaaacaggag
      301 caaatgtgtt ctataaatgt gagaatcttc aaaaaacagg ttcttttaaa ttaagaggag
      361 cttgtaacaa aatagcgagt ttaacagatg aagaaaaggc aaatggagtt atagcttcaa
      421 gtgcagggaa tcatgctcaa ggagttgcat tgggagcaaa aatgactggt ataaaatcta
      481 caatagtaat gccagcaact gcgcctttag caaaagttag tgcaactaaa ggttatggag
      541 cagaagtagt tttaaatggt gcggtttatg atgatgcata tgcaaaagct gttgaaatac
      601 aaaaagaaac aggagcaact ttcttacatc cattcaatga taagtatgtt atagcaggac
      661 aaggaactat atctcttgaa attttcgaac aattagacaa taaagttgat actatactat
      721 gcccagttgg tggaggagga atcatctctg gtgttgctgt tgcagcaaaa gcattaaatc
      781 ctaatgtaaa aatagtcggt gttcaaactg caaatatacc ttcaatgaaa gagtcaataa
      841 aaaatggaaa agtaacaaca gcatttaatg atactacaat agctgatggt atagctgtta
      901 aaactccagg agatttaact tttgaaataa taaatgaatt ggttgatgaa atagtagtgg
      961 ttgaagagac agaaattgca gaatcaatat tatttatgat ggaaagtcaa aaaatagttt
     1021 cagaaggtgc tggtgctgta tgtacagctg ctatattaag tggtaagtat gtaccagcaa
     1081 aagatgaaaa tgtagtttgt ataatatctg gtggtaatat agacataaat acgttatata
     1141 gaataatagg tgttgcttta gctaaagaag gaagaagata ttcatttagt acaattatgg
     1201 aagataagcc aggaaacttt gctgagttaa ctagaataat aagtgagaat ggtggaaaca
     1261 ttttaagtgc taatcaaggt aaattatcag caggggaagc tctaggaaaa caaagtgcgg
     1321 aatttatatt agagacaata gattatgacc atatagctag aataaagaaa gctatagaag
     1381 aaaaaggttt taaaattata gaattataat aaaaagtgta tataatatac aagaaaaata
     1441 gtataatagt gtatgaagat acattttatg tgtattagat taacgaatta ggaggatttt
     1501 aaaatgaaac atgaagtaat acatactaat gatgcaccag cagctttagg accatattct
     1561 caagctataa aagctggaaa cttgttattt gtatctggac aagttcctct tgtaccagag
     1621 actatggaag tagttgaagg agatgtacaa gctcaaactg ctcaatcttt aaaaaatctt
     1681 aaagctatat tagctgaaag tggagctgat ttttcaaatg tagttaagac tacagtattt
     1741 ataaaagata tgaatgaatt tggggctata aacgaagttt atgctgaata ttttggagaa
     1801 aataaaccag caagagcttg tgtagaagtt gctagattac caaaagacgt taaagttgaa
     1861 atagaagtaa tagctgtatt ataaaaatac aatattttta atttgaaaag gctgaaaaac
     1921 tatattctag tgattcagcc tttttataaa aaaataaatc acaataaaga acatgataaa
     1981 tattgtcgaa ttaaaaaata aaattaaaat aaatattgca atatttttga gaaggtttta
     2041 taatgagtat ataatttgaa acgtgtaact attaaattag gcatgagttc ttggatattt
     2101 ttatcctgag gattcatgcc ttttttgtat tgaaattcaa aaatctattt aataaggagg
     2161 aagtactgtg ttaaattttt tcaagaaaaa taaaagctat aagttacatg cagtagtgag
     2221 tggtaacagc attaatattg agaaagtaaa cgattctgtg ttttcaaaaa agttaatggg
     2281 agatggagtt gcaattattc ctaatagtaa tgttgttgta gccccttgta atggtaaagt
     2341 aactgtgctt acagaatcaa agcatgcctt tggtatggtc tcagatgagg gtgttgaaat
     2401 attagtccac ataggaattg atacagttag tttacagggt gaggggttta aaaatgaagt
     2461 gtctcaagga gatactgtta aaaaaggtag tccaataata agttttgaaa gagaaaaaat
     2521 aaattctcag ggaattgatt gtacaacaat aataattgta ttaaatcata gcgaattttc
     2581 agaaataaat tgcatggtag aaaatgaagt tgttgcaggg caagatactg ttattgaaat
     2641 tatgaaataa taggaaggga ttaattcaat atgagagtag taaaaatctt taataataat
     2701 gcattatcta cagtaactaa tgataggaaa gaagctattt tactaggtct tggtataggt
     2761 tttaataaac gtccaggaga taaagttaat gaagataaaa tagaaaagat ttattatgtt
     2821 caagaccaca tgcaaacaaa atttttagaa ttattaaaaa atgttagtcc agaagtgatg
     2881 gatgcttcac aacaaattat aagtcttata ccaggaaatg agggaaaatt taataataaa
     2941 gggatattat ctttagtgga acatattagt tttgcaatag atagaatgaa aaataatgtt
     3001 tttttaccta atttaatgct tttggatata aagatgatgt attcaaaaga gtttgaatta
     3061 ggagtaaaag ctttagaaat tatatataga gtatgtcata tccatttgcc tgaagatgaa
     3121 gcaggttaca tagctttaca ttttgtcaat ttacaatcaa atgataattt ggcatatgat
     3181 actttaaaat ttgtaaaagg tagtattgat ttgattaaag aatgttatgg attagaatta
     3241 gacgaatcga gtttaagtac attacgtttt agaactcatc ttaaatttct tgctcaaaga
     3301 atttttaaga atgaaatttg tcaggatgat aaaatgattg aaatgtatga ttatttaatt
     3361 aacaatcatc ctaaaaataa agaatattta gaaaagttaa atgtatatat tgaaaaggag
     3421 tttaggtata aattagataa acctgaaaaa atttatctac tactacacct aacaaaaatc
     3481 ttataagaat gagatgaatt agtaatagaa atatataatt ttaggggggg ataagattga
     3541 aagaaaaact acttggagga ctggaaacat ttgcaaaggc aatggttcag ccattaatgt
     3601 atttatcgag tgcaggaata ttaatggtgg taggtgtact acttacaaat gatatgttat
     3661 taggagtact tccatttttt aaatgggagc caattcaaat atttggaagc ctaatttata
     3721 actgtataat ggctattatt aataatcttg ctgttatatt tgctataggt attcctgctg
     3781 cactagcaaa aaaagagaag cacaaagcag cgttaatagg atttatgagt tatataatat
     3841 accttacagc ttccaacact atgttaacat cactaggtca actagcaaaa gaaagcaaga
     3901 tgcttggact gatagggact ggacaagcta ctattttagg tattcaagtt gtagatacaa
     3961 gtgtatttgg tggaattatt ttagggtgta tagctggata tgtatttaat cgtacaagca
     4021 acaaaacatt taaaggagct ttacagatat actcgggagc aaattttagt tttatatgca
     4081 tgttctttgt atccatttta attggagttg tgagtaactt tatatggcca ttagctcaac
     4141 aaggaatagc ttctcttgca aatgtcatca aagatacagg tacatttggt ttattcttat
     4201 atggtttttt agagagaata ttagtaccaa ctggacttca tcatttaatc tatacaccat
     4261 ttcagttttc tgatttagga ggagtgctta aagttggtgc agatacagtt tcaggagcct
     4321 atgctatagt tatgacagaa atgaatttac cagttgagcg tttctcagat agtatatact
     4381 acatggcaac tggatttaca aagatgtttg gttatatagg tataggagct gcctttattt
     4441 atactgcata taagcaaaat aagaagaaag ttaaggcaac gattattcca ctaataatta
     4501 cggcagtatt agcctctgta acagaaccat tagactttat gtttgtattc tgtgcaccat
     4561 ttttattctt tgtacatgca gttatagctg gtctttttat agctttatta aaaatatttg
     4621 atgttactgc attatgtgga gggaacttat tagtatcaat tataatgaat actacagtag
     4681 gcgtagaaaa aacacactgg ccagtaatga tgatattagg acttgcacaa attattgtat
     4741 attttgtagt atttagtttt ttaattaaga aatttaatta taaaactcca ggtcgtgaag
     4801 atgagagtac agctacaggc gaagaacaat caaaacaact taatttagat gcagggatag
     4861 aaaatataat tgatgggtta ggcggaaaag aaaatattaa tacagtagaa aattgcatca
     4921 ctagattacg tgttaatgta aaagatgaaa gtaagattaa tgaagatata attaatttaa
     4981 cgccaaatag cgggattgta aggaaaggaa aagatataca aataattttt ggacttcatg
     5041 tgcatgaggt aagacgtgca gtagaagatt ttttagaagc ttattaaaga tatatattta
     5101 tatagcaagg gtaaataaca gctttaagtg ctgtttacta tttattaaag atacatataa
     5161 tttatagagg aggaataatt attatgatta ttactattgt tggtggagga agcacattta
     5221 ctccaggtat tgttaagtca attgcattaa gagaaaaaga gttagagctt gaggaaattc
     5281 gcttatttga cattgataaa gaacgccaag ataaagtagc tgtagttgtc aactggatac
     5341 taaaagaaga tgttaaaaca gatgttaaac taactgtaac taattgtgaa aaagaagcgt
     5401 atacagatgc tacatttata tttgcacaaa tgcgtgttgg taaatatgaa atgcgtgagc
     5461 aggatgaaaa aattccattg aaatatggtt gtgtaggtca agaaacttgt ggttgtggag
     5521 ggatggcata cggactgcgt acaatacaac caatgataaa ggttattgat gatgtagaga
     5581 aatatgctaa aaaagattat tggatattaa attactctaa tcctgctgca atcgtttctg
     5641 aagcttgtcg taagttaaga ccaaatgcaa ggattataaa tatctgtgat atgccaatag
     5701 ctattataga tatgatagcg gggtcattaa atatcaatga cgttcataat atacgctatg
     5761 attattttgg tctaaatcat tttggctggt ttacttcaat agattataaa catagagact
     5821 taatggaaga gattaaggaa tacataaaag aaaataaaat attattacca aaatcttata
     5881 tagaaaactt aaataaaagt cgtagtacag gaaatcgcca tgctgtaagt agctggatac
     5941 atgtttggga atctgtttat acaatgttag aaagcttccc aaaatatctg ccaaacactt
     6001 atttaacata ctatctttta agtaaagaaa cagtggaaca ttcaaacata aatcgtactc
     6061 gtgcaaatga ggttatggaa agccgtgaaa aagacttatt tgaaggtgtg agacattact
     6121 tagaaactgg agaaatttct gaaaaagcat tttatgcagg ttcacatgga gattggatta
     6181 gtgatttggc agtttcaatt aaaaatgata cacgtagtcg ttttttagtt ataactgaaa
     6241 atcgtggagc tataccaaac atgccatatg atgcaatggt agaaattcca gcatatattg
     6301 gtaaagatgg tccagaagtt attgctcgag gtccaatacc tctatttcaa caaggattaa
     6361 tgatgcaaca acttaacagt gaaaaattga tagtagaagc ttatatagaa aatagttatg
     6421 aaaaagcttt acaagcattc acattaaata aaactattcc aagtatgaat gttgctaaag
     6481 aaattttgga tgaaatgata gaagttaata aggaatactg gccagaatta agatagtagg
     6541 ataaaggaaa gggtttttat ttaataatta gatatgtttt cagagtcttt tatcaaaatg
     6601 tattcacaaa catatggcaa tatattaaaa taaagagtga tatttttaga agaatggggt
     6661 gatgcaatga acattataaa taaaattttt tgggaagaag ataggattgg catgattaca
     6721 aataatttag aggcagatac acatagccac tgtatgttac aactgttctt agggatagaa
     6781 gacagcattg aaataacagt caatgaaaaa ttagttaaat gtaattgtat catagtagat
     6841 aaaaatatct ctcattcatt ttcagcaaga aaaaaagttt attactctgc tattattgaa
     6901 cctacatcta tatatgcaga acagcttacc tcaaaaatga atgattttgg ctactggata
     6961 tgtgacaatg atggattaga gaaattacgt caacaaggaa cttttttgat agataactct
     7021 agtaaagaac aatatttaag atttatggaa atgcttaata actatttgaa tatacctata
     7081 actctcaaac attatgatga cagaattact gaattgctca atttattacg tacttgtaat
     7141 tgtgataatc atactatttc aagttttgca gataaagtgt ccctatcagc aagtagatta
     7201 tcacatctgt ttaaagaaca aatagggata cctttaaaaa gctatatctt atttcatcaa
     7261 atggagtgtg cttttagaga attactttct gggaaaaatg ttaccgaagc ttctatgact
     7321 gctggattcg atactccatc acactttgca gggacagtta agcgaatgat gggaatgcct
     7381 gtttctttat ctttgaaaga tagcgaattt ttgaaagtat attaaatatt ctcgttatat
     7441 aatgaagttg tactaagaaa ggggctttgt tatgaataaa gaatatttag aggaaacaaa
     7501 aatgttaaat tataatgaac cacaacttaa gcttttggta tcatcaaaga attggttgga
     7561 attagatgat tttcataaaa taaaatctat ttatgagttt gtacagaatg atattttatt
     7621 tggatataat gcttctgata tgttaagcgc aacacaggta ttaaatgatg gtatgggaca
     7681 atgcaataca aaagctactc ttttaatggc attattacgt gcagtgaata tcccttgtag
     7741 gcttcatgct tttgatgtaa caaaagattt tcaacgagga gctacatcca aattgatttc
     7801 actattagca ccaaaatata ttcttcatac ttgggtagag gtattttatc aagataggtg
     7861 gattgctctt gagggtgtca taactgataa aaaatattta gaagcaattc agaagaaatt
     7921 ttttaatcat ggtggaacat ttaaaaagta tgcaatagct acaaatgatt tgaaaaatac
     7981 atctattgat tgggatggta aagatacttt tatccaaaaa gaagctatcg tgtatgatta
     8041 tggtattttt ccatcaccag acgtattttt ttctactcat tcacaacata tgtcaaaatt
     8101 aaaaaatttc atatatgtac atcttatcag aaaaataatg acaaaaaatg tatgtaaggc
     8161 tagaaacaat tatattgata aaaacgaatg agtgtttaag aggtaactat ataggaaata
     8221 tatagaataa ttaaaactgt actggttaaa atgaataaaa aggagttatc acagagcaaa
     8281 cttaaattgt tcataggata actccttttc tgtattaact tctaataatt tgatgtcttt
     8341 ttataaaaca tatagataca attaaaccag taaatgcaag agtagctgct attgaaaatg
     8401 caacatctat tccatgaatg ttagacagca tgtttgaact catatgagct ttcttagtag
     8461 cacttgtcat tatagtaata agtatagcag aacctatagc tccagatatc tgacggagtg
     8521 tgttattaat tgctgtggca tgagatataa gttctctatc taatgtttta attccccatg
     8581 tagttaatgg cattaaaacc attgaaatac caatcatacg aatacaatac atcaatgaca
     8641 catgaattaa agatgtatct ctgcctaaaa aagcgaagga taaagttcct aaaaacaaac
     8701 atcctgttcc taatattgat agagcatgag gtccatattt atctaaatga cgtcctgcaa
     8761 ttggattgag tacaaccatc aatatagcac caggtaacat taatgaacct gaagataatg
     8821 cagaaaatcc tcttacagat tgaacgtaca gaggaatcat taatgtagca gacatcattg
     8881 aggcatatac aatcataatt aatattgtag aaataagaaa tggtttattt ttaaaaaccc
     8941 tcaattctaa aaaaggttct ttagatttta attgacgcaa tgtaaataaa attaatgaca
     9001 taattcctat taatattggt aaatatgtgg caatattagt ccacccataa ttaccttgat
     9061 tagtaaatcc aattaataaa ccaccaaaac ctagagatga tagtactatt gaaggtatat
     9121 ctaattttag tttttgagtt tccccaacat ttcttaatag aataaatgat agtataacat
     9181 ctaatatagc aataggggaa agaaacagaa ataaactatg ccaaccaaat gaatctacta
     9241 accatcctga taaagttgga cctattgctg gagcaaatcc tactgtaata ccaactaaac
     9301 tcatagctgc accacgtttt tcctcagggc ataaatagag tataataact tgtaaaagtg
     9361 gcattagaga accagaaccc attgcctgca acacacgagc aatcaacata gttgaaaaat
     9421 tattagaaaa aagtgcaata atacatccta tagaaaagat agacattgat gtaataaata
     9481 actttctagt actaaatcga tttattaaat aagctgttgt gggtacaata atacctgcaa
     9541 taagtagata aattgttgtt aaccactgac caatatcagc agtaatttta aaatctgaca
     9601 ttatattagg taatgctgtt gacaatagtg tttgactgag agaacttata aaagcaccag
     9661 tcattacaat tgctattatt aaatttcctt ttttattgtt actttgggtt tccataattt
     9721 ataacgtctc cttttttaat tgatttttaa tgctgttcaa taaaagtact tttggtctta
     9781 aagtgtcttc tttttctaaa tatgcaatca ttgcatccat aattaaagaa tcatattgtt
     9841 gattttttcc ttcgacgtat aattttttta ttgcttctag taatttagga ttttcaagga
     9901 gagaagtatc agttttgatt agttcttcta tatcttgtat cgttttaaaa aatagcgatt
     9961 tattatgaag ggaaaagtaa acttcatctt cttgtttatg taaaaacatt gatttttcaa
    10021 ttaaaggatt tagattatcc atattttcta caactaaatc tagaatttgt atagtaaatt
    10081 tggcaataaa gtatacattt atttctcttt tttgtacatg aataatgtat ataaactgat
    10141 gaattagagc atccaataag aaaactaaat caaatataat tttttctatt ttatcaccat
    10201 agttatcaaa taagcttagt ttgtattcat tcaataagtt agctcttgat tttgtttgca
    10261 atttacttgt agccttacgt tgctcttcag aaaagttttc tcttatatag gtatttatgg
    10321 tagttctaga aaaaatattt ttccatacag caattatctt tctgtttaac ttttctgatg
    10381 atggaatgtc attattttca tttatggaat gtataatttc taaaaacttt tcatcataat
    10441 aattgattat ttcgcagata aaactttcct tagtttcaaa atgcttgtaa aaagtagctt
    10501 tagacatttt acactctttt acaacatccg atattgaagt attttgaaaa cctttttggt
    10561 caaaaatttt ttgtgcaata gttataatga gcctttgttt ttctgtcact ttattatctc
    10621 ctttcacgca aaaacgttta gtactatata gtacctacaa gttttgataa atatattata
    10681 catcatttgg aaaaaaaatc aatatataga ctgtaatatg aaatttttga aaaatttgtt
    10741 gttattttta gtcgtataaa aaagaaactt aagagtttct taagaattgt ttaaagaagt
    10801 tgcaataaat tagtggtatg attatagtat gaaagagaat gtcttgaaat ttttctgtat
    10861 caaattccaa ttatggaggt gtagtatatg aaaaagaaaa tttgtgtagc aataattttt
    10921 gctctattta tagtttttgt agaaatgttt attgatagct ctagcgaagg aagtaaaatt
    10981 atgacagctg aattaagtaa atacaggaaa ggcgtaaatg tattatcaat aaacaatatt
    11041 atagtagcga ataaaaaata tagtttgccc aaagattatt ctcctcagga aagcagtgaa
    11101 gcgagagatg ctttttataa aatgaataaa gatgctcaaa agtcaggatt aaatttaaaa
    11161 gcttttagta catatagaag ttatgaatat caagatagat tatttaagtc atatgtaaaa
    11221 gaacatggag aaaaagaagc aaatagattt tcagctaaac ctggagagag tgagcatcaa
    11281 actggacttg cttttgatat tggaggagat gaccagtctt gttgggcaaa taaaaagttc
    11341 aacaatacaa aagaagctaa gtggttgtat gaaaatgcat ataaatatgg ttttatactt
    11401 agatatccag aaggtaaaga gaatataaca ggatatatgt atgaatcttg gcactataga
    11461 tatgttggaa ctgaacatag caaaaacttt gcaatgaata atttgacttt agaagaatat
    11521 ttacatataa attaagagat ggtttgttaa aattaggtta aaataaaaaa taacctagac
    11581 tattagctgt gtaaagtgat ataatgtaaa tacaatttaa gataactact cgaaggggag
    11641 tagcttacac gaaagtggta attaatcgtc aagacagtag aaatactcgg ttaattagca
    11701 agggattcct tgatagcaag accttcaaaa taagcattag gtatgtttgt tttggggtct
    11761 ttttttattc aaggaatttt tatttaaaaa aatttaaatt tcaagtatac ataagagccc
    11821 ccaaacataa aataaattta ttgaagagga gggcaacaaa tgtcttacaa aagtacttat
    11881 caagaagtaa ttaaaaattt agactcaagt ttatctggtt tgagtaaaga aagagcagaa
    11941 gagttgttag aaaaaaatgg agctaatgaa ttaaaagaag cagataaggt tcctacttat
    12001 aagcttttcc tagaaagttt taaagaccca ttagtaataa ttctattgat agctgcttta
    12061 gtacaaatat ttttaggtga aacagtggag tctataataa tttttgcagt tattattata
    12121 aactctgttc tgggtgttgt acaaacaaaa aaagcagaaa gttcattaga aagtttgaaa
    12181 aatttatctg caccaaacgc aaaggtaata agagacaata aaaaaatgac aattccagca
    12241 aaaaatctag tagttggaga tattgtattc ttagaagctg gcgattacat accagcagat
    12301 ggaaggctta tagaagcaca atctctcaag gttgtagaag ggatgttaac tggagaatca
    12361 gaaccagttt taaagcatat agaaaaaata agtgaagatg taagtttagg tgaccagaaa
    12421 aatatggtat ttagtggtgc tgtagttgtt tatggtagag gttcttttgt agtgactgga
    12481 acaggtatga atactgagat gggtaaaata gctgggcttt tagaaagtgc tgaaaataaa
    12541 cttacaccat tgcaacaaaa aatagaggat tttagtaaaa aacttggtgt aggtattgta
    12601 atacttgctc ttttaatatt tataattcaa gtagcaagaa attactttat ggctggaaat
    12661 ggagaaatga cacaaattat tttagattca ttcatgttct ctgtagcagt agcagtagca
    12721 gctataccag aagctctatc atcaatagtc actatagtat tggctgttgg aacaaatagt
    12781 atggcaaaaa aacaagccat aataagaaaa ttaccagcag tagaaacttt aggttcaaca
    12841 agtgtaatat gtactgataa aacaggtact cttacacaaa ataaaatgac agtagttgat
    12901 tattatatgt atggaactaa taatgatgag ttagataaag aaaaagtaaa ctattcttat
    12961 catgctaaat tattgactat ggtttctaca ttgtgtaatg actctcatat aactagtgat
    13021 ggtaaagaag ttggtgaccc aacagaagtg gctttaataa attattcaag taaaaatgat
    13081 ttagattatg ataaattaag aagtaagtat atcagaataa atgaaatacc atttgactca
    13141 gacagaaagc ttatgtctac tgtaaatagt atatatggag attatataat gtttacaaaa
    13201 ggtgctccag atattgtgtt tagtagatgt aaatatgctt tgaaaaatgg ttcaaaagta
    13261 gatataacag atgagataat agaagaatat aaacataaaa atgaagagtt ctcaaataga
    13321 gcacttagag tattagcttt tgcaataaaa gatgtcccag aagaagattt tataccttca
    13381 attgatgacg aacatgacat gacattggtt ggaataatgg caatgataga cccaccaaga
    13441 gaagaagtaa tggatgcagt aaaagaggca aagagtgctg gtataaaaac tgttatgata
    13501 actggtgacc ataaaacaac agcagcagca attgcaaaag agattggtat aatggaagaa
    13561 ggagacttgg ctcttactgg aaaagagctt gatgctttga gtgaagaaca actatatgaa
    13621 aaactagaaa atataagtgt atatgccaga gtctcgccgg aaaataaaat tcgtatagta
    13681 aaagcatggc aacataagaa taatataaca gcaatgactg gtgatggtgt taatgatgca
    13741 cctgcattaa aacaagcaga tataggaatc ggtatgggaa gtggtactga tgtagcaaaa
    13801 gatgcttctg ctatggtatt ggttgatgat aactttgcaa gtatagtaaa tgctgttgaa
    13861 gttggtagaa cagtatacag taatataata aaatcaatta cttatctatt tgcaggaaac
    13921 ttaggagcta tagtatccat agtatttgca gtgtttgcag gttggtcaaa tccatttaca
    13981 gctttacaac ttttgtttat aaacttagta aatgactctt taccagcaat tgcattaggg
    14041 tttgaaaagt cagaaaaaaa tataatgaat aaacctccta gacacccgga tgaaagtata
    14101 ctagcaggag gaacattgaa ggcagttgct ataagaggtt gtataatagg tgtagttaca
    14161 ataattgcac aatatatagg attacaagta tctccagaac ttggagtagc aatggcattt
    14221 tcaacattga tattagctag aataatgcaa acattacctg cacgttctaa taatcaaaca
    14281 gtaataaaat taggtttttt cagcaataaa tacgttatag gagcagtttt agtctgtctt
    14341 gggctttact ctttaacact aataccagtg ctaagaggaa tattctctat accaacaacg
    14401 tttggattgt accagttggg gatttgccta gggttagcat taatttcatc aatattaatg
    14461 gaacttaaca aattaataaa tagaagtgaa gtaaaataat caaaaaatta aagggcatat
    14521 tgattttaac aatatgcctt ttataaatag tctaatttat tctatattat cgattatttg
    14581 gaatgctttt ataacttcag gtaattcttt ttcttcaggc attctaaatc ttataaaatt
    14641 tttatcaagt ccagtaaaat cacttccaga tataactctt atttttaatt tagcaaactc
    14701 ttgttgtaaa tctatgtcct tgtttttatg ttcaactgtc ataattgata cagtatcact
    14761 agtttcagct atattaaggt ttttccatgg aattaataat tgatttttta tatcttttgt
    14821 tttttctttt aattcttcta taaattgaac atcatctaat aaatttgctg caatactacg
    14881 agatatttca gacacttcgt aaggagtaga aatcttttta atagggctaa caagttgttc
    14941 tggaagaact gcatatcctg ctcttagtcc tgctaaacca aatccttttg aaaatgtttt
    15001 taaagcaatt acattatcat aattatttag aagcttgaca gctgaatttt cttttggcat
    15061 gtattcacca taagcttcat ctaccataac tgctatatca tatttagctg cttcccttac
    15121 tatattttca attgatgata aaggtataat ttgtcctgta ggattgtttg gattgtctat
    15181 gtaaattact ttatattctg gatttatttt ttcaataaat tctttttcat taaacttgaa
    15241 attatcctct ttctttaaaa gaacataatc ataagttgca ccatgcattt ttatatctgt
    15301 ctcatattct gagaactgag gtgaatatcc taaaacttta tctccctttt caataaatag
    15361 tctatttaaa aggtaaataa catgtataga accatctgcc aaacaaattc tgtcagtatc
    15421 taatgctata aaatctttcc aataatcgac tatattatct tttaaagcaa ttgaatgagg
    15481 atatcctctt atcatatcaa attttaaagc attaaaagct tctacagctt tgtttgagta
    15541 agacacggta tttatacctt caccacagtc tatctcgaat acatcttctg aatgtccaga
    15601 ttctattgca taactttttt tcaactgcat ttcaatagct ttgttaattt ttagtttcat
    15661 actttatcac tccttattta gtcaataata aatgttataa aagttgctag atttaaaata
    15721 ttatgaaaat atataatagt aaaaatgaat ataaatatta taatagctca ctttatttaa
    15781 tatgattata tatcatatat tgtaaaattt ctagcattat aagactataa attgaaaata
    15841 ttcagaaaaa tactacaaga aacgatttat tttgctctta aaataagtta aatgaatgtt
    15901 attaattttg taaattgaaa tagtttaagt ataaagttat gatataatct aataagggta
    15961 attctttaga ttatattaag gaaatatagg tatacaataa aaagttatgg agttggatta
    16021 tgggatataa attaattgta acagatatgg atggaacttt attaggcaat aatcataaag
    16081 ttacagatga aaataaaact gcactacaaa aagttataaa aagcggtatt aatgtaactt
    16141 tagcaacagg aagagccttc gattcagcta aatgcaatgt agattttctt aaagaggaca
    16201 tgccaataat agcttgtaat ggttcattaa taagagagca aaatggaaat ataatatatt
    16261 caaataagat tgatacaaga acatgtttaa acattttaga tgtattagat aaatacgata
    16321 tttactacca atgtaatagt atagattcta tgctttcgaa aaaaatagaa ggacgtgaag
    16381 atagattaag tgtattttta ggctctgaaa cagaagtaat tgtaaaagat gatttgagag
    16441 aagaaatctt taaaaaagat atactaaaat ttgtaatcat agaagaaaaa aatccatcta
    16501 ttttggatga aataagaaaa gaattgagaa aagttcaagg aataaagata acctcttcat
    16561 ggccaaacaa tattgaagtc atgaatgaag gtgttgataa aggtaatgca gttaaaattt
    16621 tagctgaaaa aatgaatata gacagagaag atataattgc atttggagac aattacaatg
    16681 acatagaaat gataaagttt gctggtcttg gggtagctat gggaaatgca gaagaattga
    16741 ttaaacaaga agctgattat gttacagata caaatcaaga cagtggagtt gcaaaggcaa
    16801 tttataaatt cctagagtta aaagaatcac ttagctctta aaacagtata taataggaaa
    16861 aaataggttg actattttgt agatagtaat tataatacaa atagataaaa tataaaaatt
    16921 tagattcagt gaaaaagaga gtagatatag agaaaatctc agcgaattgg gggatggtgt
    16981 gagcctaatg ataacacttt atattgaaaa gaactttgga gaatactact tgaaattcag
    17041 tatggtagta ggtaatctcg ccttaagaga atagagtggg taaacattgt ttaccaacta
    17101 gagtggtaac acggaatatt tcgtctcttg gtctttttga ccaagagatt ttttagtttc
    17161 attgagttac aagacttata attaaaatac aaaaaattaa aatatgaggt gaaagttatg
    17221 aaattatggg gtggacgctt tagaaaagcg gaaaatcaac ttatggaaga atttaataaa
    17281 tcttttggct atgattgtgt actgtacaaa aaagatatcg aaggaagtgt agcacatgtt
    17341 catatgcaag taaaatgtgg acttttaaca gaagaagaag gaaagtctat aacagaaggt
    17401 ttaaaaggga tacttgaaga tgtagaaaat ggaaagttag tattggatgg agagtatgaa
    17461 gatatacata gttttactga gataaattta attcaaagaa ttggagatgt tggcaagaag
    17521 cttcatacag cacgtagtag aaatgaccaa gttgcagttg atatgagatt gtatgctaaa
    17581 gaaaaagcaa atgatttggt ggatttaata tctgaattta aagctactat aaaagatgtt
    17641 gctgacaaaa acccagtgat gatgccaggc tatactcatc ttcaaagggc acaagtagtg
    17701 acatttaagc atcacttaat ggcatattat agtatgtttg atagagatga gaaaagaata
    17761 aaaaatgcta tagaaatatt agatgaatca cctttaggct gtggagcttt agcaggaact
    17821 acgcatgata tagatagaag tataacttgt gaacaattag gatttaaaaa agtagtagat
    17881 aattttatgg atggagtaag tgatagagat tatttactag agctaatgag tgatttttca
    17941 attataatga tgcacttaag tagattaagt gaagagttga tactttggag ttctcaagaa
    18001 tttggatttg ttgagataga tgatttatat acaactggaa gtagtataat gcctcagaag
    18061 aaaaatccag atggtgcaga gcttataaga ggtaagactg gtagagtata tggaaactta
    18121 tttggtcttt ttacagtaat gaaaggaata ccattagcat ataataaaga catgcaagaa
    18181 gataaagaag gttttttcga tagtgtacat actcttgaaa tgtgtataca aataatggat
    18241 agaatgatag ccacactaaa ggtaaatgag gataaaatga aacaagcagt aaaaaatggt
    18301 tttttaaatg caacagaagt tgcggattat ttggttaaaa ataatgttgc ttttagagat
    18361 gcacatggta tagtaggaag tatcgttata tattgtgaag ataataaaaa agcaatagaa
    18421 gatttaacat tagaagaatt acaaaagttt tcagatgctt ttaaggaaga tatttatgat
    18481 tttatcgact atgagagtat attaaataag ggaataaaaa agaatttaaa ataatgtatt
    18541 tatttttaaa agctgtaaat ttgaatgtta tcatttaaat ttacagcttt ttattgtgta
    18601 agattttatt atataaaagt aagaaactta aaaggagtag tatttaaaat aaatacaaat
    18661 aaaaaattat tattaaaatg ataattaata tcaataagta ttgacaaaat tatttctaaa
    18721 tactattata tataatataa ataataatga aaatcaatat caaataaatg ggaggaaatc
    18781 taatgaactt attagtgatt ggtggtcatg aaagaatgga aaaagattat atggttatgg
    18841 ccaagaaaaa aggttataag acaaaggtat atactaccat gtcatctaaa ttaaataatt
    18901 ctataggaaa accagatgca gttgtcatac ttacatcaac tgtttcacat aagatgtcta
    18961 gaacggttga gtctcaagct aaaaaacaag atatattaat cgtaaggcat aaaaatagta
    19021 gtaaggtagc atttaatgaa tgtctagata tggttgatga atgtctggga aattgttcaa
    19081 aatgtaagct taaagacaaa caaaaacaaa aaaatatata ggtataaaaa atacataaac
    19141 ataaaattaa aagtactgag ataatagtta taattattat ctcagtactt ttagatttat
    19201 aaggataagt atagtataaa ttttttatta taatttccaa ctacttgtta ctttttcata
    19261 ttttttttac ttctagcttt ttttcttttt ttcttcttta tatttgaagc tattataaat
    19321 aaaactaaaa gcactagaat acctgcaata atcttaacaa aagtcattaa aacattattt
    19381 tcagtcaaaa atggaagact gctatcaaga ttattttttg caattaagtc tatactgttt
    19441 tctagtttac cattattata tacatttaaa gtaccaacct tagctccttt ttttataggc
    19501 aaatcaattt tatttaactc tgttttggta tcataattgc cttgagattc atctttttct
    19561 aagaatatcc tataactatt ttttggttca taaatcaatt catgttgttt tgtaaacaat
    19621 acttttttag tatctgtgta tttttcttta tcaacaatag tagcacactt aaagttatca
    19681 aatccataat caatcagaat acgtgagtct aaatataaat cagcattagt tgaattaaat
    19741 acagcaacga taactcttct gccatctttt acagcactcg ataaaagaca tttaccagca
    19801 gcatcagtat atccagtttt tataccatca actatatcat attttatatt tataggttga
    19861 cctttgtatg taatcttatc atgtgatgtc aaaaacttat tagtgtttac aaagtatctc
    19921 tcataaggat atgcttttgt agcttcaaat ttaagtgact tagtttttac aatttgtcta
    19981 aaagtatcat tattcatagc ttctctagct atgagagcca tatcatgagc tgttgtgtag
    20041 tgatttgggt ctggtaatcc atgaggatta ttaaaatgag tattagtagc tccaattttt
    20101 tttgcctcag agttcatgag gtcaataaat ttttgtacat tgccaccacc aacatatcta
    20161 gctaatacaa aagcagcatc attagcagaa tgaaccaaaa gagcatctag taactgcttt
    20221 acagtaaaag attccccatt ttttagatac atactagagc catctatttg aggtaaatct
    20281 ttaatttcta ttacttgatt taaatcttta acctccttta gaactaaaca agctgtccat
    20341 actttagttg ttgaagctgg ataaagctta gaatttccat ccttattata tagtattttg
    20401 ccagactcat agtccattaa aactgcatgt ttagcaacta tagctggttc attatctgca
    20461 aaagagatat tagagcttcc taaaaatgaa gtaaatatca ataaacagat taataaaagt
    20521 gataaatttc ttttcattgt gagcctccaa attttaaatt catcttttag tatagcatat
    20581 atttgtcaat aattaaaaga aaggagtgat taatatatga aaaaaaaggt aaaatttatt
    20641 tcgttttttg ttttatttat tttaataata tcatcaataa tcgtttataa atcgcttatt
    20701 ttcaaagatg atatttatgt tgtaagtaaa aaaaatttac ctaatgaaaa aacacatgtt
    20761 gtagagaatg aaattaaagg tgaagataat aatcacagta aagtaactaa agacatagat
    20821 aaaaattttc aaaataataa aataactata tacataagtg gagcagtaaa tagacctgga
    20881 attgtaacta tagagtcaga caagagactt tatgatgctg tagaattact aggaggaacg
    20941 acaaaagaag cagatttaaa tggagtcaat ctatcagtaa gattagaaga tgaacaacat
    21001 tacataatac ctaaaatagg tgaagcaaca tctgttacaa gtaatgatga ttccaataaa
    21061 ctaaatcaga agaatgagtc aagaggtgag cctaaaaata aatccaataa aaatatatct
    21121 aacgaatcaa aagtaaatat aaatgtagcg acgatagaag agttggattc tctacctggg
    21181 gttggagaag ctacagccaa taaaatttta caacacagag aagaaaatgg acagtttagt
    21241 tcaattgagg aaataaaaaa tgtaaatggt attggagata aaaagtatga aaatataaaa
    21301 gatttaattt gtgtagacta aaatataata caaaacttac atatagatat gttattataa
    21361 atatgtatgt atatgtgtgt acatgtggaa atttagtaaa ggaggcactt ttatgaaaaa
    21421 agaacaaaaa aaacttacag agatgacaac agcaggtggt tgagccgcta aaatagggcc
    21481 agaggttctg gcatcagttt tatctcaatt gcctaaaaat gataatatag aaaatttatt
    21541 agtaggacta gatacagcag atgatgctgc agtttataaa ttaaatgatg atatggcact
    21601 tatccaaact ttagacttct ttacaccaat ggtagatgac ccatacgtat ttggtcaaat
    21661 tgctgcatca aattcactat cagatgtata tgctatggga ggcaaacctt tggttgctat
    21721 gaatattgtt tgttttccat catgccatga tatggatgta cttgcagaaa tattaaaagg
    21781 tgggtttgat aaagtgaaag aaagtggagc acttttggtt ggagggcata ctgtagatga
    21841 caaagagcct aaatatggtc tttcagtttc tgggattgta agtccaaata aagttttatc
    21901 aaatgctact gcaaaacctg gtgataagtt aattataaca aaaccaattg gagtgggtgt
    21961 attaaataca gccatgaaag aaggtatggt agaacaacat atagcagata aagtaataga
    22021 aataatgata catttaaata aatatgcagc aatgagtttt gagaaatttg atgtaaattc
    22081 ggttacagat ataactggtt ttggtctact tggtcatact ttagaaatgg ccaaagcttc
    22141 tgaagtaagt attgaaattg aatcaaaaca tgtaccaatt attgaaggtg caatagaaat
    22201 ggcacaaatg ggaattatac ctgctggtat gtataaaaat atgcattatg tatctaaaga
    22261 tgtagaagtt gtaggaaata tagaagttgc agttcaagat attttatacg acccacaaac
    22321 atcaggaggg ttgttaattt cagtaaaaga agaacttgca gaggagcttg taaaagatat
    22381 gaaattaaat ggagcaatag aagctaaaat tataggttct gtagttccaa aaggggaaaa
    22441 atatattaca gttttataaa atattataga gaaagagtga ttgttttgag caaaagagaa
    22501 ttgtttgcaa tgttgccttc agttgatgaa gtattaagtg ataatagaat tgtagaaata
    22561 ataaatgaat atcctagaag tttagtttta gaatctgtga gagaagtaat agatttaaaa
    22621 agacaattta tattaagatt aaaagaagat gcatcaaact ctgtaacaat agagtttggt
    22681 gaaataatag aatctgcaat agagagagtt aagttaaatt actcattatc tttaaaaaaa
    22741 gttataaatg caactggtac agttattcat acaaatttag gaaggtcttt acttagtgaa
    22801 gatataaaag atgaattatg gtgtgctgct tcaaggtatt caaacttaga atacgatttg
    22861 gataatggag aaagaggttc aagatactct catttaacta gtacaataaa aagacttact
    22921 ggagcagaag atgtactagt tgtaaataat aatgcagcgg cagtacttct tgtacttagc
    22981 acaatggcta aaggaggaga agcaattgta tctcgtggag aattagttga agtaggtggt
    23041 tcatttagga ttccaagtat aatggctctt agtggtgcag aattagttga agttggttca
    23101 actaataaaa ctcatttgaa agattacaag gaggccatta cagaagatac aaatgtattg
    23161 atgaaggttc atacaagtaa ttatagaata atgggattca cagagagtgt ttctatagaa
    23221 gaattggtta atttaggtaa aaaatataaa ctacctgtaa tagaagactt aggaagtgga
    23281 gtttttatag atttatcaaa atatggttta tcttatgaac caactgtatt ggattctatt
    23341 cgacaaggtg cagatgtagt aacttttagt ggagataaga tgcttggagg tccgcaagca
    23401 ggtattatag ttgggaaaaa agagtatata gaaaaaatga agaaaaatca acttactaga
    23461 gcacttagag ttgataagct tacaatatgt gctcttgaag caacattgag aatgtattta
    23521 gatgagacaa aagctataga aaatatacct actttaaaaa tgttgacata taaaattgaa
    23581 gaattagaag taaaagcaaa taaactattt gaaaaaataa ctgctttaaa cttaaatgca
    23641 aacataaata tagaagatgg attttcacag gtgggaggag ggtctatgcc tcttgaaact
    23701 ataagtacaa aggtaatatc aataactcct gaacatatga atgtttcatc tttagaaaaa
    23761 aaactaagac ttagtgaagc acatataata gccagagtgt atgataataa atatgtacta
    23821 gatgtaagaa ctatatttga tgatgaattt gatgtaatag ttgaggaatt gagaaaagca
    23881 tttaattaaa taaatttgag ttaataaata ttaaatttag aagtataagt atgaagtttt
    23941 gattaaaaaa tagctacaca cacttgtgac tttagtcata gcaggtttag agggggcaaa
    24001 aaatgaaaaa tgtagtaatt ggaacagcag gtcatattga ccacggaaaa actactctta
    24061 taaaagcatt gactggaaga gagactgata cgttagatga agaaaaaaag agaggtattt
    24121 caataaactt aggatttact tattttgatt taccaaacaa tagaagagct ggaattgttg
    24181 atgtcccagg acacgaaaaa tttataaaaa acatgttagc tggagcatca ggaattgata
    24241 tagttttact tgttgtagca gcagatgaag gggttatgcc acagacagtt gagcatttag
    24301 atattctaag ctttttaaat atcaaaaatg gaatagtggt tctaactaag tctgatactg
    24361 tagatgatga atttagagag ctagtaaaag aagatatttg tgaaaaaata aaaggaactt
    24421 ttcttgaaga tgctgaaata attgaagttg attctatatc taaaagaggt atagacactc
    24481 ttattgaaaa aatagataaa atgagtgatg atatagaaga taaaaatgaa aactctccag
    24541 caagacttaa tatagacaga gtattttcaa taaaagggtt tggtactgta ataacaggca
    24601 ctttaataga aggaaagata agtatagaag atgatttggt tatatatcca caggtattaa
    24661 aaacaaaaat aagaagtata caagttcatg gagaaaataa agatacagct tatgcaggtc
    24721 aaagaacagc aatcaatata tcaaatgtaa aagtggatga tataaaaaga ggagatgttt
    24781 tagctgcacc aaactcattg gaggaatcaa tgatgttaga tgtaaaatta tcattggtaa
    24841 atcatttaga taaaggatta aagcactggg atagattaag attgtatcat ggaactagag
    24901 agatactttg tagagcagtt ccactagata aagaattaat tgaaagtgga gaaagtggct
    24961 atgttcagtt gagacttgaa gaaagcatag tatctaagaa aggtgatacg tttgtagtga
    25021 gacgttattc tccaatggaa actataggtg gaggtgtaat aattgaccca tctcctaaaa
    25081 aacataagaa atttgatgaa aaagttatag aagcattaaa aataaaagaa aaaggcgaac
    25141 taaaagatat aatagaagag tacttaaaaa ggaatttaaa aaattatcca aatataaaag
    25201 aaataatgag ctatagtggt gctcatgaag aagatgtaaa aagagcgctt gaaacactta
    25261 tatcagaaga taaagtattt attataggaa atatgtatat gcatataaat caatacaata
    25321 aattaaaaga aaatacaata aagcttttaa gtgagtacca taagaagtat aggcttagaa
    25381 aagggatact aaaagaagaa gtaaggtcta agatagagag taattttaag actagggaga
    25441 tggatatttt attagaaaaa ttgtctacag aaaatgctat aaaaatagaa aataatatag
    25501 tatctctatt agattttgag gttattttaa atgataagca aaaagaaata gcaaaaaaaa
    25561 tagaaaaaag attaaaatca tgtggagttt cttctatact gacaatagat gaagtttctg
    25621 aaggaaatca taactatgct gaagtattgg agtcaatgat aggtaataaa gtagagaaat
    25681 tagatgattt gtatataatg gacaaagata tttatgaaaa tgcaaagaac attttgataa
    25741 attatataaa agaaaataaa gaaataacat taggagaata cagagatttg attgattcta
    25801 gcagaaagaa ttgcatgatt atattggaaa acttcgatag aaacaaaata actaaaagag
    25861 tagaaaacaa aagaattttg ttttagcaga aaatgtctgt ttttgcttta aggtataaag
    25921 aaggaattat aatgtcgagt atcgtataat taattagtat aaaatattaa ttaagaggaa
    25981 gtgtaaagta ttgaagaata taatacctga tgtaaaacca gatataaaag aaagagaaga
    26041 catgagcctt ttatttataa taatgggctc agtactttta ttatttatta taaaagatat
    26101 agagaaatat gaaactttat tacatgccat atatacgata tttttagtag ctacaggagt
    26161 aattatattt aatactacaa aatttaaagt agattcattt tctacatttt taggattaat
    26221 ttttatagct actggagtat tggagtgtat atatttattt aattccttgg ggattaaaac
    26281 taagtctata atggagatta acatcacaat atcagcaata acagatttat ttcctatact
    26341 gggtgtatac ttaagtttta aatttgttaa ggataacaaa caaatttatt caagtgtaat
    26401 tttatttata gcaactgcta cattaacaat aacatcttta ttcataatat caggaatttg
    26461 tgactatctg ggaaatggta aatcacaata tgctttagga gttataatat ctatattcat
    26521 aataattatt tctatcatta gtggaattga attaaattca agttcaaata aaggtaaatg
    26581 ggaatatgat gaaaaaaaga tttttaacag aataatagta ataataatct tatcaagagt
    26641 tccaaatcta ttgcatatag ttattgataa tagaaatgta gagaggatat tatcacaaat
    26701 aataataaat gtagccttat attatttata taattacata gtatctaaga atattaagaa
    26761 aacagcactg gaattgcatg atacgaatga agaattaaca aaaaaaacgg aaagtttaaa
    26821 agaaaagaat aaaaaattaa tctatgagac agaaaagata gaggaactta agggaatact
    26881 tatgaaaaga gaatctaggc tacaatctac attagatgta gcagtaaata gcatagtagt
    26941 atttagcaaa gatggagaaa ttacatatgc aaataagagc tttagaaata cttttggaga
    27001 ctataaagaa caagataaat tgactgataa aataaaaaac ttcaatgatt tgatagaaag
    27061 cattcattat gtgtttgttc atgagaagaa tgcagagaaa cttattagta caagcgataa
    27121 taaagtttat caagctatat ttgcaccttt aattattgct tcacaaacag aaggagtatt
    27181 gtgtgtacta gaagataaga ctaagaaaaa ggagtacgaa cgcaaactta ttgaagcaaa
    27241 taagagaagt aaagattttt tagaaagtat tggagatggc atagtagtgc ttgaaggtaa
    27301 taacaaaata tatgttaata atgcatgtag ggaaattttt aaaaataatt tagagagtat
    27361 tgatttttca ttagtatgta aaagtgaaga aagtatagaa aaaagatatg taatagatgg
    27421 tgaagtaaag tatgtagaga tgagtttttc tcagtatact aatgctggta ctaataaaac
    27481 tattattgta atacgtgata caacctctcg aaaaatatct caaataaaac ttgaggaaag
    27541 tcaatcatca tattcaagat ttatagatat attacctgat ggcatatgct tattaaaaga
    27601 agatttaagt ataaactatg caaataagtc gtttttagat atgctagctt ttacaaacat
    27661 agatgatata aaagattcta atattaaact attaatgaat gcgaatactg aagaaaaaat
    27721 gaaatttacg gataatatgg ctaaagtttt aaatgaaaat agaagtatac ttttactgga
    27781 acatgaattg ataaatagtt atgatgatat agtagaagta gaattaagtg cattaccttt
    27841 tgctatatac aatactagat acataatgct tattataaaa gatttagtac ataagaaata
    27901 ttccgagcaa gcagaaaaag aactgcttga aagatttaaa acagataaaa tcaaaacgga
    27961 gttttttgca aatatgtctc atgaactaaa aactccacta aatgttatat ctagttcaaa
    28021 tcaactggta gattctttct atagaaatga aaagataaaa gattacaata acaatattaa
    28081 atctcatgtg gatttagtta gacaaagttc ttataggctt caaagattaa taaataacat
    28141 tatagattta acaaagatgg agtctgggtt ctatacacta aaacttgcga aatacaatat
    28201 tgtatctgta atagaagact tatttatgaa tatagaagag tatgcactta gaaaagacat
    28261 aaaaattctt tttgatacag accttgaaga aattaatgtt tatatagaca aggttgaaat
    28321 tgagagaatt atgttgaatc tattgtcaaa ctgtattaag tttactgata atggaggctg
    28381 gatatatgta agtattcatt ataaaataga taaagtaatc ataagtgtaa aagacactgg
    28441 agttggaata ccacaagata aattagagtt gatttttgaa gaattttcac aagttgataa
    28501 gactttatcc agaaatactg aaggaagtgg aatagggctt gctatagtta agaatctagt
    28561 aagtcttcat ggtggagata tagaagtagt aagtgaagtg aataaaggca cagagttctt
    28621 aatttcactt ccaatgaagg gatttagtag tgaacattac acagaagata agagaatata
    28681 taatattcaa gaaaaaataa aaatagaatt ttctgatata tattattaaa aatgattatg
    28741 agcaaaaata aaataaaaat actatttgtt taaatattta agttgtaaga aataggctat
    28801 cataaggtaa attattatga tagcctattt ttagctaggt tttatatcta tatgtgagaa
    28861 catataatct ctaaattacc ttcaaaacta aaatctccaa agtcgtttgg cagtataaaa
    28921 tgcataccct tttttaaatt atattctaca gaattgtgta taagttttcc atttccatcc
    28981 aacacactgt ataaagaaaa aggtgtattg tgtgtaaaat tacattttcc aaaaacatct
    29041 aatttataaa cactaaaaaa ttcgtttgac acaaatgttg tgcatttaaa gtctgatgtt
    29101 gatacgattt tatagtcagt atcgaaattt atatgaggaa cattcgtaac atctattgat
    29161 ttttgaacat gaagttcacg cttattacct gaattgtcag ttctatcata atcatagact
    29221 ctataagtag tatctgagtt ttgttgagtt tctaaaatca atgttccttt gcatatagca
    29281 tgtatagtgc cacttggaac atagaaaaag tctccctttt ttatattaac ctttctaagt
    29341 agtttgtccc attcattatt atttaccata tttacaaatt ctttatgaga ttttgcatta
    29401 tgacctatta taatttctgc atcatcagaa caatctatta cataccaaca ctctgtctta
    29461 cctaactctc cattttcatt ttttttagca tattcatcat ttggatgaac ttgaactgat
    29521 aaattatcat ttgcatccaa tatttttgta agtaaaggaa acttatcacc aggtgtgttt
    29581 ccaaataatt ctctattttt attccacaat tcagatagtt ttttaccttt atattttcca
    29641 ttttctatta agcaatctcc atttttatga gagcttatag cccaacattc accagttgtt
    29701 ggcgaatcta tttcataatt aaatttatct ttaagtgcag taccacccca tattctatcc
    29761 ataaaaatag gttttaaaaa taatggttcc ataatcctgt tcctcctatg atattaaatt
    29821 tatcctacaa ataattatat ataaatagat tataaaatca atagtaaaaa agttttgtcc
    29881 cttgaaatga cataaagtat aaggagttat taaataagat aaactatgat atataatata
    29941 aagttctaaa ttggagtgat aattatggag ttgtttaagg cagaaagcaa aaaatataaa
    30001 aaagaatttt taaaattatt ggagcaatgg gttagtatac cttcatttta tgatagaaaa
    30061 actgtatcta aagacatgcc ttttggaaaa ggagtatatg atgcattaaa ctggtttgaa
    30121 aatctaggta gagaaaacaa tttcaaagta aaaaatatag ataatcatgc tgttcaaatt
    30181 gaatatggaa atggtaaaga gtatgtagat atatttggac attgtgatgt tgtaaatcca
    30241 ggtgaaggat gggatagtga accatttaaa ctaaatatta taggtgataa attagttgct
    30301 agaggtgtat ctgataataa agggcctatg atagtgaatt ttttagcact aaaaatgata
    30361 aaggatttag atattaattt aaaaagaaag gttagattaa tagctggggg aaatgaagag
    30421 agtggattta aatgtataaa gcattattat agcaaagagc catatggtgt atgtggattt
    30481 actccagatg ctaaatttcc tgtattaaat ggagaaaaag gtggagcaat aataaagtta
    30541 atttcaaata tagacgataa atctttatat ataagtggtg gaatagagtt taatacaata
    30601 ccagataaag tttatataaa gaatgtagaa aaattgggta aagataatat atgttttgat
    30661 attaacaata tatcgataaa ttatgataat ggcaattata ttgtgtatgg aaaaggtggt
    30721 cattcatcta aacccgaaaa atcaataaat cctatattag ctacaattaa acttttgtca
    30781 gaaaatattg atgaaaaatg gacaaaagat ttatataagt tgataaatca agataatata
    30841 aatggaaact tatttggttt aaatattgag gggaaatgtg gaattttatc aatggtgcca
    30901 acaataataa atatagtaga tggaaaatta gaagtagttt taagcgttag atatccagaa
    30961 atattaacca ttgaagatat aataaaaaaa tttaatttgt atatggagca aaataatatt
    31021 aataaatttg agcttattgg ggaaaattta aagcaagcaa attatataga tagaaattca
    31081 aaacttgtaa gaagtttgca tgatatatat ataaagtatt ctggagattt aaaaaatgat
    31141 gttagagtaa ctagtgcagg aagttatgct tctgaaatga ataattcagt aatatttggt
    31201 tgtgaatttc ctgatggtag ctttggcaat gtccatagtg cgaatgaatt tgctagctta
    31261 gatagattta taactgcaat aggtatatat gcagaggcaa taattacatt atgtaataaa
    31321 atataaatac aaaatgtccc ttatatagac atattatata tacaattaaa attttacttg
    31381 atatataatt gaaatcaaag agaaggtgat acaatgataa ctaagaaaca tgcaataata
    31441 gtaaagagtt taagcaataa agatggatat atgacatcaa acgaattagc tatcaaattg
    31501 gatgtgtcta ctaaaactat taaaagatat atagcagatt taaatagtgt tttaagcaaa
    31561 tatgatttgg taatagcttc ttctagaggt ataggataca agttaagcgg ttctaaaaac
    31621 aatatagcca gagctgttaa agaagcaaat aaatatattg atggtttttt agatgactct
    31681 gaagagtcaa gaatgtcaaa tataatatgt atgcttatta atagaaatta tatgagtata
    31741 gaagcaatgg cagaagaatt aaatctaagt atagccgcta taaataaatt gtctagtaag
    31801 cttaaaagaa aacttgaaaa gtatgactta gttataaaat ctaaacctta ctatggaagc
    31861 tatatacatg gtgaagaaat taatataagg caattaatta ctgactatgc aataaaatta
    31921 gatgaaaaaa gtaaagtgaa ggtgttttta gatgacatta gtgaaaacga tatacactgt
    31981 atagaaagta ttttagaaaa gcacttaaga gaaaaagata caataatttc agataaagat
    32041 tttaatcttt tgctttcaaa aataatcgtt tctgtattta ggagtaagag aggtcatagt
    32101 aacaatataa acttaatgga tacaagttat agatttcata actactactt tatagaaaat
    32161 ctaatgaaag aaatatctga taaaattgga tttaaactta ttgaagatga agtaatttat
    32221 atttcaaatt attctggtgt aatagcctat aaaggtacac aaggaagaaa aaacacaagt
    32281 gaaattgagg aacgaataag tagtgttata agtagtgcac ttcaagatat attccttatt
    32341 tcaggtagtg attatacaaa agatgatgag tttatggttg caatctctga ccatataaag
    32401 agatttttaa atagagctag agctaatgta aaatcaaata atcctttact tcatcaaata
    32461 aaagagaagt ttcctatagc ttttaattta gccgtgtttt tatcaaataa actagaaact
    32521 gagtttaatt taaaacttga tgaagatgag cttggatata tagctatcca ctttgctgct
    32581 tcaaatgaaa gaatgaaaaa aaatactagt aagaaaattt gtatagtttg tcattatggg
    32641 ataggaacag gacaacttat ttctgaaaag ctaaaacaga atataagtga tttaagtgtt
    32701 gtaggtgttt atccagttag atatctcgat atggctataa gtcaagatgt tgatttgata
    32761 gtatctaccg ttgaattaaa aggatatgaa aaacctgtct tatatataga aaatatattt
    32821 gatgatagct taattgaaaa cgtaaacaaa gccttttatg aaaaggaaga aagaagaaag
    32881 ataataagca acatgtttga tgaaaaagct tttttcagta taaaggctaa tactaaagaa
    32941 gaggtcataa tttcactttc aaataaacta aaagaaagag attttataga ggaaagttct
    33001 ataaaaagta taatagatag agaaaatatt tcatcaactg aaataggaaa tctagtagca
    33061 ataccccaca caatagtgaa gggagataaa aaatctataa taggagttgg aatacttgaa
    33121 aatccaataa tttgggataa gcaagaggtt cagctagtat ttatggtatt ttttaatact
    33181 aaagaaaaac ataatttctc gatatttaaa tacctttata attttataaa agatgaaggc
    33241 ggagtaagag gaattattaa aatatgtgac tttaataaat taatggcact gataggtaat
    33301 taaggaggaa attaataatg ggaacaaaca tatttaacaa agaatatgta tttttaaatg
    33361 ttgatgctaa gtcaaaggtt gaagttttaa aatttatatc aaaaaaagct aaagacttaa
    33421 acttggcaga agatgagagc ttagtatatg aaggtttaat ggctagagaa gaacaattta
    33481 ctacaaattt aggagaatct atagcaatac ctcacactaa aaatgatgca attgaaaatc
    33541 cagcagtagt agtattgaag tttaatgaag atgttgtttg gaatgaaggt gaggataagg
    33601 ttaaattagc aataagctta cttatgccag gaaaaagtaa agagaatatt catcttaaat
    33661 tattatcatc tctttcaaga aaattaataa ataaagaatt taaagattct cttttaaaaa
    33721 gtgataatgt tgaagaaata tctaacttaa taaatgaagc tttaggttta taattaaaca
    33781 ctaaggttat aaaataaaaa aataattaac taaaaatagg aggaatttga aatgaataaa
    33841 aaattagtag cattatgtgc ttgtccaatg ggactagctc acactttcat ggcagcagaa
    33901 gcaatagagc aagcagcaaa ggcattaggt tatgaagcaa aagttgaaac tcaaggtgct
    33961 gatggtgtac aaaatgagtt aacaagagat gatatattag gagcaactat gataatacat
    34021 gcagtagcaa taacaccaga aggtatggaa agatttgatg gttgtgaagt ttatgaagtt
    34081 gaattacaag aagctataaa aaatgcagaa ggtgttataa aagaaataga agaagattta
    34141 ggaatataat aaatttattg tggaggaaat agaatggcaa ttaagaaaaa agttataaac
    34201 agttcaggtg cttctacaac aggaggaaat aatgtaggaa aatctactcc atctagtcca
    34261 actcaatcaa agggaaatgg aaaatggaaa gaaataagta aacatataat gacaggtata
    34321 tcatatatga taccagtttt agtaatgggt ggacttatag gggccttatc tcaattaata
    34381 ccatatgcta tattaggact tgacccatct gtaggtatag ttgatgctat gaactcagga
    34441 gaatttacag ggttcaagct tagtctttta aatatagcac aattaatgtc aaatttcgga
    34501 tttactttgt ttggatttgc gataccactg tttgcagcat tttgtgcaaa ctctataggt
    34561 ggaaaaacag ctttaattgc aggatttata ggtggatata tagctaataa acctgtaggt
    34621 gtaccacaat tcgttgatgg acaatggact gaagttgttc cagttgcatc aggattttta
    34681 ggtgcaatac taatagcatt tataatagga tattttgtaa aatggttaaa taaatctata
    34741 aaagtttctc ataactggtt agcttttaaa acaacatttt taataccatt aatagcgtca
    34801 ttagcttgta tggtattgat gatatttata ataactccat ttggtggatt aattaatgaa
    34861 agtatgaaaa acttcttaac agcagcagga gcagctggag aatatgtata tgcaacagct
    34921 ttggcagcag ctacagcatt cgacttagga ggaccaataa ataaagcagc aggattcgtt
    34981 gcacttggtt taactacaga gaatgtactt ccaataacag caagaacaat agctatagtt
    35041 gttcctccaa ttggattagg gttaacaaca ttattagata aaagattagt aggaagaaga
    35101 gtatatgata gacaattcta tcaagcagga aaaacttcta tattcttatc atttatgggt
    35161 atatctgaag gagcaatacc atttgcactt gagagaccag gttttgttat acctttaaac
    35221 atagttggaa gtgtaatagg agctattaca ggtataattc taggagcaat tcaatggttc
    35281 ccagagtcag ctatatgggc atggcctcta gtagataact tatttggata tataataggt
    35341 atagcagttg gtgctatatt catagcagta ggaaatatat tctatagaaa taaattaata
    35401 aaagatggta aacttgttgt agattatatc gactagtatt taatttttac tatctataaa
    35461 taactttggc tggtaatatg gttttaatca atattaccag ccttacctat atgattaatt
    35521 aaaggcggtg gagataagat atggataaga tagaaaaagt aagagagtat tttagagaat
    35581 atgatataga tggatttctt ataaatagtc ctacaaacaa gttttatgtt ggaaatttat
    35641 ttagctcttc aggttatgtt tttataacga aagaatcaca atatataata gtagatttta
    35701 gatattttga agaaattaaa agaaaatcta gtttgtttaa tgtagtatta atggacaaaa
    35761 ctagaactca ttttgatata attaatgata tttgtagaga gcagaatata aaggaaattg
    35821 gatttgaggg aaatgaagta tcttttgatt tatatagaag tatgagtaat aagttaagtg
    35881 caaccttaaa atcagttgat ttatctacat taagggaaac aaaaaatgaa gacgaaataa
    35941 aatatataaa aaaagcttgt gaaatagttg atgctacatt ttatcatata gtagacttta
    36001 taaaggttgg tatgactgaa aagcaagttg aaaatgaaat tgttagaatt ataaaagatt
    36061 taggtggtca aaaagaatct ttcgatacaa tagttgcatc tgggcttaga ggagcattac
    36121 ctcatggaaa agcaagtgaa aaagttatag agtatggaga ttttgttacc tttgactttg
    36181 gtgctaagta taataattat tgttcagata ttaccagaac catttgtatg ggtactataa
    36241 ataaagagtt agaagaaata tataatatag taagaaaagc taatgaagag tgtataaggg
    36301 ttttaagacc gggaatgact actggagaga tagataaagt tgcaagagac ataattggtt
    36361 catatggata tgctggtaat tttggtcata atctgggtca tggtgtagga attatggttc
    36421 atgaatatcc tgcattagct cctgaatcaa atgaagtatt aaaagaagga atgattgtaa
    36481 ctatagagcc tggtatatat gttccaagtc ttggaggcgt aagaatagaa gatgatgtac
    36541 ttataacaca agatggatgt atgagattga ctacatctac taaggattta atagttgtaa
    36601 gctaaaaata aattagataa atattttata acaataagaa aagaagaggt aaatttaaat
    36661 gtataatttt gatgataaac cagatagagt aagtgaaaag tgtagaaaat gggatttaaa
    36721 tattataaga gataaatttg gagatattag agaagatttt ataccaatgt ggattgctga
    36781 tatggatttt aaaataccaa cagaaataga aaacaaattt attgaagctg taagaagagg
    36841 tgtatttgga tatacatact gttatgatga attttatgat tcagttattg gttggcaaaa
    36901 aaatatgcat gaggtaaatg tagaaaaaga atggattaca ttaacttatg gaactgtatc
    36961 aacacttcat tatgtagtac aagctttttg taagaaaggt gatagtataa tattaaatac
    37021 accagtttat gacccctttg aatcatcagc aaaaaaacaa ggtgtaaatg taatttgtaa
    37081 tacattagat gttgtaaatg atagatatta tataaatttt gataaattag aagttcaaat
    37141 taaagaaaat aagcctaaat taatgatgtt ttgtacacca cataatccat ctggaaggat
    37201 atggacaatt gaagaaatga caagggtagc aactatatgt aaagaaaata atgttatttt
    37261 ggttgcagat gaagtacatg cagagcatat tcattatggt acatttaatt caatacttaa
    37321 gattggaaaa gagcttttag aaaatgttat tctattgaca tcaccaaata aaggatttaa
    37381 tcttggtgga ttaaagactt cttattcaat agttataaat aaggatataa gggacaaatt
    37441 tagaaataaa ttaaagcaaa attctataac ttctccaaat gtgtttggaa taataggatt
    37501 gataaccgct tataatgaat gtcatgagtg gttaagtggt gtaaatgaat atataaaatc
    37561 aaattatgaa cttttagaaa cttgggtaaa taaatataat aagataaaat tgatgaaaat
    37621 ggaatcatct tacttagctt ggatggatat aagtggatta ggtattagtg caagtgagtt
    37681 tacagataaa ttggctatag atacaggagt attattagaa gatgggagtc attttgtaaa
    37741 agatggtgag aagtatgtta gaataaattt aggaactcaa aaggaaaatg taattgaagc
    37801 tttaaataga atggatttat ttttaaagtc tttataaaaa tttggttgaa ttagtatttt
    37861 atataaattt aaactattaa tatggtatat acataagtag aatttgtttt agattactta
    37921 agtatatacc atattttata taaaaattaa atttgcatta gtcatgtgat aactatttaa
    37981 attcatatgt attttgtatg tataatagat ataattatta tttaaactta ttgtttattt
    38041 aatttttcaa tagaaaaaga tttatctgta agtttaaaat tatcttttat acccttagtt
    38101 aagtatactt tcttatcctt agttatgaat atagcatcta cattatcaat tttattaatt
    38161 aaattcatac ctttttcaag accaagacca aatgtagaag tggataaggc atcacaatta
    38221 atagatttat cagagattat tgtaacacta cttaaattgt tttcaaaagg ataccctgta
    38281 aatggattta acatatgatg atatattttt ccgttttttt cgataaatct ttcataaatt
    38341 ccagaagtaa caacagactt atttgtagtt tctatgtttc ctatggattc accattttta
    38401 ctagttgggt cttgaatacc tattttaaaa ggtttatcat ttttaccatt ttctaaagta
    38461 aatatatttc ctcctaagtt tactaaacct ttttttacgt cctctgattt taaataggct
    38521 acaattttat cagcagcata tcctttagct atggcaccta aatcaatttt catattttct
    38581 ttggttaatt ttatagattt atttttttca tccaatatga tgttcttata atcaataaga
    38641 ggcagaagtt tttgtatctc agaatcactt ggaactttag ctttatctgt tccaatagcc
    38701 cataaatttg ataatggacc aattgttatg tcaaaatatc catctgataa tttggaatat
    38761 tgtatagctt cttttacgac gaaaaatgta tcatcagaaa ctctgacaaa ttttttacca
    38821 gcattatcat ttattttaga aacatcactt cctggtatat gagtgctcat tttgttgtca
    38881 atatatctta aaattgaatc acattcattt aagattttat cacttttgga ttcttttaca
    38941 ttaaatacag taacttcgtt tactgtacca aggtagtagc tagttttact atatttagta
    39001 ttctggcttg aactcctaaa aaatgaaaat aaaattaatg ctaaaatcaa tatcacaaaa
    39061 ataaatgtga tttttttctt attcatggta tattcctcct atgaaattgt gttattattt
    39121 tattataaca taatttcata ggaggacaaa aaatgaagaa aaaagacatt cttcttattt
    39181 taggcatttt ggttgttatt acagcatgtt atgggataat aaatgttata aattctaaaa
    39241 atgctggaaa tattgagatt tatgtagata ataaattgta taaaactgtt tcaataaatg
    39301 ccaaagaaga atttaaaata gagaatagag gggggtataa catagtaaag attcatgata
    39361 aaggggttga gatagtagat gcttcatgtc ctgataaggt ttgcgtacat actggattta
    39421 taaacaagcc aagtcaaagc atagtttgta tacctaatag agtgagcata aaaataaata
    39481 caaatgaaaa gaatgataat caagaagata taatttctaa ataaaataat aaaaataatt
    39541 gaaaggaatt ggtgataatt atggttaact taggaaacga ttgggatgaa ttattgaaag
    39601 aagaatttga aaaagattac tacttaaact taagaaaatt tcttatagaa gaatataaaa
    39661 cacgccaaat tttcccaaat atgcacaata tatatgaagc tttaaaacac acttcataca
    39721 aagacacaaa agtcttaata ttaggacaag acccttatca tggagataat caagctcatg
    39781 gtttagcttt ttcagttcaa ccacaagtga aaactccacc atctttatta aatatgtata
    39841 aagaattaaa agatgattta ggttgtttta tacctaataa tggatattta atgccttggg
    39901 cagaccaagg agtgttactt ttaaatactg ctcttactgt tagagcgcat gaagctaatt
    39961 ctcataagaa caaaggatgg gaaattttca ctgatagagt aatatctatt ttaagtgagc
    40021 gtgaagaccc agttattttt gtactatggg gaagtaatgc acgtaaaaaa gtagagttga
    40081 ttgatacaag taaacattat attttggaag cacctcatcc aagcccattg tcagcaagta
    40141 aaggcttttt tggatgtaaa cacttctcta agataaatga aattttgaaa aagctaggaa
    40201 aagaacctat taattggcag atagaaaata tataaaagta aaaggtaaca aaatgataac
    40261 aaaacaacaa taaacaatga cattgttgta actaatagaa atatgcttaa atagttgaaa
    40321 atactatgta tgcatatttc tatttttttg tcaagttgac aactcttgaa aaaatagtta
    40381 caatttagtt acacaaaaaa cacaaataaa aaaagaaact tacaaagcag aattttatga
    40441 agcaaaatat atagtgtaaa aaatcttagg gaggaaattt tatatgaatt ttaaaaaatt
    40501 gagtaatgct aaagtctcgg taatggcagt tgttttatca gttggaatat tatcgggata
    40561 ctctgcgctt aataaacaag taacattagt agtaagggga gaagaaagaa ctatatcaac
    40621 atttagttct aatgtaggag accttttaga atctcaaaac attagctatg atgcaaatga
    40681 tatagtaact ttagatgaaa atacaaagtt atctaatgga gataaaatag aagtaattga
    40741 tgtaaaggaa aaaacagttg tagaaactaa agacattcca tttgaagtta cagttgttga
    40801 agatggctct ttattaaagg gtgattctaa ggttaaagaa gaaggaaagt ctggtaaaag
    40861 tgaattaata tataaagaaa cttatcacaa tggaaagaaa gtagaaaagg aatttgtgaa
    40921 aaaggttgtt aaagctgagc cagtaaataa ggttgtaagc aaagggacaa aggtagaaat
    40981 gcaagtagcc tcatcaaggg gagaaagttc aagaagaatg gcttcatcat catctagtag
    41041 cagtagtagt aacaatggta aaaatatgag agtggtagca actgcatata ctggtcacag
    41101 tataactgca acaggaacaa aacctaagtg gggaacaata gctgtagacc ctaaggttat
    41161 accatatgga actaaagttt atatacctca atttgacatg gtgtttactg cagaagactg
    41221 tggtggagca ataaaaggaa ataaaattga tatctacatg aatgattcaa aaacagttta
    41281 taactgggga agaaaaacta tagatatacg tatattaaaa tagttaaata atttatattt
    41341 ttactggaaa ttatattttg attatataga gaagaaactc tttatagatt ctaaaaatat
    41401 aatttccagt tttttatttt ggatatgtaa taaaacaggt aaattttaac catgtataga
    41461 taaaagctat ttaaattata atttactatc atataaatct ataaaaattg ttgcaaagag
    41521 tcaaaatata tgatattata gatttcgttg agaaggattt cattatggga gtggatgggt
    41581 gccggtgtgc cctctagtct tcaaaactag tatgaggggt taagagcctc ttaggtgggt
    41641 tcgattccca cgcattcccg ccaatatgta aaaggtatct tttaattaaa agataccttt
    41701 ttaaatttgt tttaagataa tttagtttta atgatatata atattttgta ttatataatg
    41761 taaattttat aatactatta ttgttcagat aaaagtctag gagagtgaaa agatggaaaa
    41821 taattttaca acgagaacta gttttctagt tggagatgat gggatagaaa aacttaataa
    41881 ttcaaatata atagtatttg gagttggagg agttggtagt tttactgttg aagctttagc
    41941 aagagctgga gtaggcaata ttactattgt agattttgat gatgtagata taaccaatat
    42001 aaatagacaa ataccagccc tacattcaac tgtaggaaga tacaaagttg atgtcatgga
    42061 agaaagaata ttagatataa atcctaatat caatataaaa aaaataagaa gtctttataa
    42121 taaagatact agtgacgaaa tactaactga aagatatgat tatgttgtgg atgcgataga
    42181 catggtaagt tccaaaatac atctaattga aacttgtgaa aaaaaaggat tgaaaataat
    42241 aagttctatg ggtatgggaa ataaattaga cccaactaaa atagtcgtta cagatataca
    42301 caaaacaagt acttgtcctc tagctaaagt tatgaggaaa gaacttaggg atagaggtat
    42361 caaaaagtta aaagtagtat attctactga acaaccaata gaacttaaga agaaagtaat
    42421 gaatggtaga aaagtaacac ctggtagtgt atcatttgtc ccatctgtag gaggtttaat
    42481 aatcgcatct gtaatagtaa atgaactatt aggacaataa gttttaaata ttttaattta
    42541 taaattttat atacaaattt gaaggctaga aggatgaata taaagcaatt ttatattcat
    42601 acttttagcc tagttatttt atattacctt attttatgta gttgtagctt tattttttag
    42661 attatgtatc tatttcataa ttatattttg taaatataat ttctccatca ctataaaaaa
    42721 caatatctcc tattctgtct gttctataaa ttgatacatg gttttgtttt agattattaa
    42781 taacctccct atgtggatga ccatattgat ttttatatcc acatgatatt gcagctatat
    42841 ctggactaat tttttttata aattctaaag atgaagaaga attacttcca tgatgaccta
    42901 tttttataaa attaatatta tttaatttaa aagaatgtaa aatttctttt tcattttctt
    42961 cttcagcatc acccataaat aaaaaagaat tatcattaaa agttaatttg aaaactatag
    43021 aatttaaatt agattcttct tgtatatatg atggacttaa tacatatata tttatattgt
    43081 tatctatgtt taaaacatca tttttataga gatgctgaat agataagttt ttatccgtac
    43141 aagaatttat tagattttga taagcctcac tatcagttga ttgttcaggc atatatatgg
    43201 agtttacatt aaattttttt ataatattat caagacttcc tatgtggtct gaatctggat
    43261 gagttgctat tataatatca atagttttta ttcttttttg tctaagataa ctaataatta
    43321 tattttcgct atcttcatca cctccatcaa ttaatatatt cttatttgtg ggtgtttgaa
    43381 ctaaaatact atcaccttgt ccaacatcaa tcatatgtat agatagtaga gattttttgt
    43441 cacagccgat taaaagtgat attataataa gtattgataa aaaaatttta tttctcaata
    43501 aaaaatcact ccattcatta catgtttata
//
LOCUS       DAYAPA010000019        43356 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.019, whole genome shotgun sequence.
ACCESSION   DAYAPA010000019 DAYAPA010000000
VERSION     DAYAPA010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 43356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43356
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.019"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(9..1964)
                     /locus_tag="ACGI59_000937"
     CDS             complement(9..1964)
                     /locus_tag="ACGI59_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9738741.1"
                     /translation="MKKYEIFIFMSIIVLCFISFPHKVFGQEENCLEDFERWVQENKN
                     NKEEIVYTLSCDMVIDEEFRFYIPYDSNFTIDTNKYKILIKNHGRFIIDDNELNIIGE
                     GGKEGVIHIENGGSISIGINNIIATDGTALYVEEGGDLHIKSSYESVEKIKANGKDAI
                     GIYTENDIRILNKDIEVNGEHAIGVYSKGDVEIEETSIKAYSSNNESGLLKDNNKLAQ
                     SIISESKKVYIIDDYNELIPSIAEDSGYDIVKCCHRGIGVFSDEIKVSKDDKIENIKF
                     PKYMVLETSSGNRLDIDVEWDFTDYYEKLEKGENFNITGKFKTEMLNKEKIIINDDVV
                     PILNVSVIEKKPIDNLELEFQNTKNGYVAVLFYDMPYSASKVFVEYSSDGINWISEEQ
                     EDIRDQAILFFEDFKLRCFRVKVVGGLKEGYSNIVFKPGFIMGGGDNQETPDDEREND
                     DIDGDRGGGGRDDPDRDEDDNNQDGNNGNENNDSNNNQNNNNENNNNENDNSDEKQPN
                     TPKDDVESSTDSNENKNPHDIPKDGNKENNSDKEKGFYNKNSITENDDNYDLSSKLVF
                     GTNEHSFPYRESISNKNQDTNKNKSNISDYDGIINNNYKLESKEYNLKKDIIFLFCIL
                     TPIMIGSILIVNPDTRKSIIKFIKLKK"
     gene            complement(2147..3622)
                     /gene="spoIVA"
                     /locus_tag="ACGI59_000938"
     CDS             complement(2147..3622)
                     /gene="spoIVA"
                     /locus_tag="ACGI59_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage IV sporulation protein A"
                     /protein_id="HFG9738742.1"
                     /translation="MNNNIYEDISKRTQGDIYIGVVGPVRTGKSTFIRKFMEKLVIPN
                     IDNEFKKDRTRDEIPQSGSGKTIMTVEPKFVPADGVEIKIKDTVSLKVRMVDCVGYIV
                     EGALGHEEGGKQRLVSTPWSQEAMTFEKAAEIGTKKVIKDHSTIGIVVLTDGSVTGID
                     RKSYVEPEERVIQELKNLKKPFAVVLNTLSPKSEETSMLRSELEEKYEVPVLPMNVVE
                     MEEEDIEEVMEAVLYDFPLTEIRINLPKWVEGLERNHWIKSSIITTLKQSIIDIGKIR
                     DIEGIIQGFSELEFLEDTGVDNVELGEGVINIDLQTKQDLFYNVLEEKSGFKIEGDYQ
                     LLSLITRLSKVKNEYDKIESALIDAKIKGYGVVAPSLEELSLEEPEIMKQGKQYGIKL
                     KANAPSLHIIKADISTEVSPIVGNQNQGEEMIKYLMEVFEEQPADLWESNMFGKSLHD
                     LVKEQLQSKLYTMPEEIRVKMQKTLQKIVNEGSSNIITILL"
     gene            complement(3794..5089)
                     /locus_tag="ACGI59_000939"
     CDS             complement(3794..5089)
                     /locus_tag="ACGI59_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HFG9738743.1"
                     /translation="MSVEVNKPSIWKNFKVLIILFVAGAFIYALPYLKNYYYDTFAQA
                     FNLTNTQMGSLGSIYGLLAMVSYLFGGFLADRISARKLLSGSLIITGISGLVLTLYPP
                     YIVVFLIHALWGITTILTFWPALVKAVRMLASENEQGKAFGFMESGRGITNAIHLSLV
                     LILFGYISSKVSDESGLTAIIVLYSAINVIVGVLVFFKLKDNEKEVSAKFDKEAFFAV
                     IKMPHTWIITIIMFCSYSANMSFYYFTPYSTEAFGATVVFASAISILAQYCRPVASAA
                     SGLLGDKVGSSKIISYGFILMIAGLLGVIFTPTKSSMIYILLIGCVAIYVSMYAIQGL
                     HYSLLEEGNYSLSISGTAIGIVATLGYLPEVLCPLAAGKILDAFPGVTGYRYYFIVLT
                     IVAIIGLVFTFIWMNMTKERRKELIEMNNNSNENTTKVS"
     gene            complement(5273..6718)
                     /locus_tag="ACGI59_000940"
     CDS             complement(5273..6718)
                     /locus_tag="ACGI59_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFG9738744.1"
                     /translation="MSLNKFEIGGITVKKKIGFWDLVFMNISALFGIRWIAKSTASSF
                     GLGLGSIPVWLLFAFIFFVPASLICAELAATYPKDGGLGEWVKQAYGEKWGFMVSWLN
                     WTSKIFWYSSFLTFLAINIAYMLGNPDLSNNKMFILALSLIIFWLLSLVSTRGMAFGK
                     FFTNTGALGSTIPAILLIVMAFMSVVILKKAPSASIYTIQNIIPKIDANSLVSISAII
                     FALSGAETTANFITEMDNAKKNFPKAILTVAVLIGGIYILGSVAITMILPPDKIAAST
                     GILDALAKVAQDLGIGSWFIRIVAFGITLSVLGALILYIAGPVKMLFGNVREGVFPKQ
                     LTVTNKYNIPSNAVIVQAIIVSLLLVGTNLLPSVDNIYNVLVTMTALTALFPYVLLFT
                     SYIRLRKTRPNEERPYSIAKSDSVCINIARMVLVVTVVGILFSTAPVMETLKDNIIYE
                     IEMIGGGLFVIITGLMLWKNYEKKISNNSGC"
     gene            complement(6734..7873)
                     /locus_tag="ACGI59_000941"
     CDS             complement(6734..7873)
                     /locus_tag="ACGI59_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HFG9738745.1"
                     /translation="MSNNMRLERLRKYMNEKSIEIALIFEPDNQFYISGFKAITYSRP
                     IVTVVTQDKIELIVPGLEELHAKEVAKVDNVYVYYEIPEMKEHGISHKHYLDIILNKY
                     PKGTTVGIEKDIVSASFSEYITDKSFAIKDVGSKIFEMRYVKDAKEIEFLKIAGYLSD
                     IGIKGSLENVRVGMSELEFDVAGDNALLKYVSENYPDTYIGFANWTCSGIDRTAQPHL
                     DSNTRILQRGDIVIHSRQVWYENYRAENERTFIIGKPTERQKEVFKIAVEAQQAGLDT
                     IKAGIPARMVDEAARAVVAKYGLELYSNHRIGHGLGLSEHEEPYLRFDNELILEEGMV
                     FSMEPGIYIPGVGGFRHSDTAIVGKNGATIITNYPRSVEELILDI"
     gene            8192..8842
                     /locus_tag="ACGI59_000942"
     CDS             8192..8842
                     /locus_tag="ACGI59_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFG9738746.1"
                     /translation="MTNKELQKKRILMYFIEAAQNIMENEGIENITLRKVADMAGYNS
                     STLYKYFKNLDHLISFASIKYFKDYNLNISRCIENVNDEYKKYIIMWKLFCKHSFDNA
                     QAFYQVFFNLSSDELSYITKQYYDMFPEDLGIHDSDISLMITGESIKERNKILLNNLV
                     DTGYIPAKDLDIINDIIISFYQNLLFLKKNTGKNSDDDELTMKMISSIEYIIKHAK"
     gene            complement(8999..10018)
                     /locus_tag="ACGI59_000943"
     CDS             complement(8999..10018)
                     /locus_tag="ACGI59_000943"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFG9738747.1"
                     /translation="MEKVCVLGTGSWGSALALGLAKKGNDVSMWTRKEEQAKKINRTK
                     ENTDYLPGVLFPNNITISTDIEKTIKDCKVIVLAVPSQAVRSTCQKIKPFIKEGQVIV
                     NVAKGLEKGTGLRLSQVCEEELPQNPYVILSGPSHAEEVARDIPTTVVVASKDLKIAQ
                     MIQDLFMSPKLRVYTNPDIVGVELGGALKNIIAFGAGICDGLGYGDNAKAALMTRGIS
                     EMSRLGIAMGANMSTFAGLSGIGDLIVTCTSMHSRNRRAGILIGKGMSLEDTLKEVKM
                     VVEGITATEVAHDVAEKLDIDMPITNAIYSVIKKGSNPKEVGIELMMRSKKHEMEEVV
                     LGDDI"
     gene            complement(10049..10663)
                     /gene="plsY"
                     /locus_tag="ACGI59_000944"
     CDS             complement(10049..10663)
                     /gene="plsY"
                     /locus_tag="ACGI59_000944"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFG9738748.1"
                     /translation="MEIFSYIIIAVVAYLLGNISTSYIVAKRIAGVDIRTQGSGNAGS
                     TNVLRTLGKRAGAMTFLGDVLKGVMAVLISEFAARLVGIDTLLAGYLAVICVVAGHNW
                     PAVLGFRGGKGVATSLGAMLAVNPVITLMCLAVFILVVAITKYVSLGSVVGIGCSPIF
                     MIMVKNKAGLIVALFLTASVIYNHRANIKRLLNGTERKIGQKKE"
     gene            complement(10698..12023)
                     /gene="der"
                     /locus_tag="ACGI59_000945"
     CDS             complement(10698..12023)
                     /gene="der"
                     /locus_tag="ACGI59_000945"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFG9738749.1"
                     /translation="MSISRPVVAVVGRPNVGKSTIFNKFAGKRISIVENTPGVTRDRI
                     FAEVEWLDKYFTLVDTGGIEPDSEDIILSQMRNQAMLAMDMSHVILFIVDGKAGITAA
                     DKEIAQLLRKTKKPVILVVNKIDSQSQFDNIYDFYELGFGTPFAVSGANSMGFGDLLD
                     EIVENFPAGLDTEYEEDIIRVAITGKPNAGKSSILNKILGEERVIVSPIAGTTRDAID
                     TYFEKNGQKFLLIDTAGLRRKSKIYETIEKYSVIRAMSAVDRADVVLIVIDALEGVTE
                     QDTKVAGIAHDEGKGCIFVINKWDLIEKDNKTMSNYTKDIKEKFPFMMYAPIVFVSAK
                     TNQRMNKILDTVEYVSNEHSKRISTSALNEVIGEAVMLNQPPSDKGRRLKIYYGTQTD
                     IRPPKITLFINDKDLTHFSYQRYLENKIRENFGFEGTSIKFEYRQKNKK"
     gene            complement(12051..13379)
                     /locus_tag="ACGI59_000946"
     CDS             complement(12051..13379)
                     /locus_tag="ACGI59_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HFG9738750.1"
                     /translation="MEVNVKKVNNIISKVYKDSIAEEMGIEVGDLLISVNEQPIHDII
                     EYRFLLSDEYLDVEIQKKDGEVYIYEIEKDYDEDLGVEFTNPIIDQAKSCRNKCMFCF
                     IDQLPEGMRETLYFKDDDSRLSFLQGNFVTLTNMSEEDINNIIKYRISPINISVHTTN
                     PELRQKMISNKFAGKLYGIMKRLADAHIEMNCQIVLCPGINDGKELDRTIKELAQLYP
                     YVNSVAIVPVGITKHRENLVELNIFNDKSASKTIEQIHQIQQKYLEKLGTRFAFLSDE
                     FYILSNSELPGYEEYEGFLQFEDGVGMIRKLKTEIEEYLNILPEKILKREKKVSIATG
                     HSAYEFIQSMADAMMDKFKNLQVNVYEIKNKFFGETITVSGLLTAKDLKEQLEDKELG
                     EALYITRSMLKADEEIFLDNIELNQLEELMRIKIIPCLNEGKDFVDKILK"
     gene            complement(13617..13817)
                     /locus_tag="ACGI59_000947"
     CDS             complement(13617..13817)
                     /locus_tag="ACGI59_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9738751.1"
                     /translation="MGMDIGLNEYTLAIVTLDKNMKNGGGCPIFYANDEKDLQNKALL
                     MSKCVGGMVHDMTNGTLIIVKH"
     gene            complement(13819..14751)
                     /locus_tag="ACGI59_000948"
     CDS             complement(13819..14751)
                     /locus_tag="ACGI59_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIEGIA family protein"
                     /protein_id="HFG9738752.1"
                     /translation="MDRVFFVAVIIGITSRIIMLNLDQKQYPTQPNVLLSQLILAFVA
                     SSLGALLVPALINRSYTSITFLSLAAEQFRQVRANRRNTLQNLEEVQLVKRGAAFIEE
                     IARTYEVRNYMCIVTSFLTIGLYYVILAETKISEGMSLVISSICGLVLAFILKKLLTR
                     KSIGDIADVMPAEISFVDESILKVGDLKGITNIGLKSDREVYINRGVGIQIIPKNKDY
                     ANAGILFDSGQRQAIVYNIYSRLGLYGDYGEPSFVPLPRRNKEDESIMIAYLPVDKNV
                     EKVMEAVKSCPILSSSKGKNLSLKNYKIGKKGSA"
     gene            complement(14796..15728)
                     /locus_tag="ACGI59_000949"
     CDS             complement(14796..15728)
                     /locus_tag="ACGI59_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIEGIA family protein"
                     /protein_id="HFG9738753.1"
                     /translation="MEESLLNANLFRHSFIVALVVGILCRGLVLRVTDKQYPSRPQDY
                     LEQIIISGLSASLGAIALPALIDKEFAALTFFAVAIQQFQGLAEQERITLKNIDNEEL
                     VPKGDAYVEEIASTYESRSYISLFSALVSSIVYIVFARKYGLSFFYCTILAIVSGAIV
                     GLIFRRFLRRNSIADIADIVPAKISFEGPILMVNGVIITNIGLESTREKYRNECLAIE
                     VIPRDLGAFGIVNDIGQRQAIIHNLFIHMGIDRDVDEVDIVAISKTNLEKSTVVIPYM
                     PILKDIDVMIDVVKSTPIIETSKGKQSDFSRKTL"
     gene            complement(15783..17072)
                     /locus_tag="ACGI59_000950"
     CDS             complement(15783..17072)
                     /locus_tag="ACGI59_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFG9738754.1"
                     /translation="MEDLNSIYFFIVMLSIAIVILAVRYTITLRKYLKEFIYVSKKVS
                     NNEYHARLNISAKGELGELARNFNHMIKNMDKTLEEVQYKHLQLRSILKSISHGILAI
                     DVNGNILLINDEAKEMIKSEPNQLVEGHNINVVIKEEEILKQILQFIGSKDNETANIT
                     TKDDIVYKIKVDPVYLQNTNNVIIGSIVNIEDITKKVKLENMRSDFVANVSHELKTPL
                     TSISGFVETLKSNENIDVNTRNRFLEIIESESDRLKRLIDDILLLSFVENKDTLLMEN
                     VVVYDVFKEVYEITQHSASEKNIEVLYKCDDKSISILSNKDYIKQVFLNLVDNAIKYT
                     QDNGIVQVVVLKDEEKIVIKVIDNGVGIPKEDVERIFERFYRVDKARSRDVGGTGLGL
                     AITKHIVKSLNGCIKVKSELGIGSEFIVTILQKNFLK"
     gene            complement(17101..17793)
                     /locus_tag="ACGI59_000951"
     CDS             complement(17101..17793)
                     /locus_tag="ACGI59_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFG9738755.1"
                     /translation="MNTKVLVIDDEMHIVELLKFNLEVSNYEVSYSYDGFDGFIKAKE
                     IKPDLILLDWMLPNISGIEVLRKIRSDKDLKNIPVIMLTAKNMENDKVEGLEIGADDY
                     ITKPFSIKELLARISSVLRRYNLTSLGEENNILTTGNLKLDLSKHEVTKGSEKIELTL
                     KEFELLKLLIQNKGKVLSRNYLLDKIWGYEYYGETRTVDVHIRYLRKKIEDEDKSEKY
                     IETIRGVGYKID"
     gene            complement(17808..18569)
                     /gene="pgeF"
                     /locus_tag="ACGI59_000952"
     CDS             complement(17808..18569)
                     /gene="pgeF"
                     /locus_tag="ACGI59_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HFG9738756.1"
                     /translation="MMKDYINIKNVQDIKEYVNLSGNCEELDEKLNSVNLVITCKNID
                     AKNREDMLKVCKECDLTFENLTSNSQIHSDIVNIVNKDTIGKRRDGDALITNLEKVPL
                     LLFTADCVPISIIDAKNKAIGLAHAGWRGTFSNIGHKTIKLMSECYKTVPEDLVCIIG
                     PSIGPCCYEVSEDLVEKFNTILTNNDEKFYIIKEGSYYLDLWKINEYMLRCSGVKKEN
                     IINLNLCTSCRADKFHSYRKHNKASERIGTVLQIK"
     gene            complement(18566..19027)
                     /gene="nrdR"
                     /locus_tag="ACGI59_000953"
     CDS             complement(18566..19027)
                     /gene="nrdR"
                     /locus_tag="ACGI59_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFG9738757.1"
                     /translation="MQCPYCNYKESKVIDSRHTDLKSIRRRRECESCKKRFTTYEKIE
                     TTPLMVIKKDNSREYFDREKIKYGLLKACEKRPVSIDEIESIVVHIENEINKCFIEEI
                     ETKKIGEMVMDKLKELDEVAYVRFASVYRQFKDINTFVNELKSILIEKGDK"
     gene            complement(19049..19312)
                     /locus_tag="ACGI59_000954"
     CDS             complement(19049..19312)
                     /locus_tag="ACGI59_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HFG9738758.1"
                     /translation="MIRVSDIMEKEIINVKNGKRMGFIIDIDMDIHEGKVVSFTIFGD
                     GSRSFFSRGGDEEVIFWNDILKIGCDTIIVNIGSELSLDEFEI"
     gene            complement(19416..20189)
                     /gene="sigG"
                     /locus_tag="ACGI59_000955"
     CDS             complement(19416..20189)
                     /gene="sigG"
                     /locus_tag="ACGI59_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigG"
                     /protein_id="HFG9738759.1"
                     /translation="MQVNKVEICGVNTSELPVLKNKQMKELLLQIKNGDEEARQQFVR
                     GNLRLVLSVIKKFNNRGENIDDLFQIGCIGLIKAIDNFDLSQNVRFSTYAVPMIIGEI
                     RRYLRDNNPIRVSRSLKDIAYKALQVRERLIRTNSKEPTVSEIAKELELEVESVVMAL
                     DAIQDPISLFDPVYQDNGDAIFVMDQVQDKKDTDENWLQEISLKEAIKKLNSREKLVL
                     DLRFYKGRTQIEVADEIGISQAQVSRIEKNALKNMRKYV"
     gene            complement(20281..21003)
                     /gene="sigE"
                     /locus_tag="ACGI59_000956"
     CDS             complement(20281..21003)
                     /gene="sigE"
                     /locus_tag="ACGI59_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigE"
                     /protein_id="HFG9738760.1"
                     /translation="MLRLKERIISFITMLGIKLKIIKPKGIYYMGGANILPPPLKPEE
                     EMELLQKLETDESVKSILIERNLRLVVYISRKFENTGIDVEDLISIGTIGLIKAVNTF
                     KLNKNIKLATYASRCIENEILMYLRKNNKKKTEVSFDEPLNIDLDGNELLLSDVLGTE
                     NDEIYKIIEEEIDRDLLVMALDRLSDREKQIMELRFGLIDKGIEKTQKEVAGMLGISQ
                     SYISRLEKKIISRLQKEMKKFV"
     gene            complement(21028..21873)
                     /locus_tag="ACGI59_000957"
     CDS             complement(21028..21873)
                     /locus_tag="ACGI59_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E processing peptidase SpoIIGA"
                     /protein_id="HFG9738761.1"
                     /translation="MVYIEYYVIENLVINYIIISCTSILTKRYNPKKNKLLGASLGGA
                     YSVAYLYPSMNILFTVPFKIIIMSIITLISFRSKSKKDYVHVALVFYLVNIFISGSTY
                     FIIYFTGISHLKISFLIACTYVSCELLKYIYKDLKMLKYIKELTKTININLLEKSFCC
                     KALVDSGNLLKDPISQSDVIIVKSSVLEGFIPEKLLNINYEDIDMKKAQEIIGSLDDK
                     TSGRVRIIPYKHAGSNKTSIIIGLKADYIEVDEQKIGNIILGISNFNDREYGAIMNPN
                     ILPGF"
     gene            complement(22190..23464)
                     /locus_tag="ACGI59_000958"
     CDS             complement(22190..23464)
                     /locus_tag="ACGI59_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HFG9738762.1"
                     /translation="MIKLKKVLSLVMVGILSVSILAGCSKKEDSKGKEGKEKLVVWSA
                     PLTDHDADAWKPIFEKFEKENNCEIEFQIVPWDNYAEKYATAISAGEGPDIGYMYAEM
                     FPQFIEMGAVEDLTPYLEKSGTSDNYLYLDDAKMMGGIYGLPIEAANPGVLYYNKDIL
                     EKLGEKPPKTWDDFKRICEKATKDTDGDGKIDQWGLAQGWGSKVFGSLNWNWYPYLWQ
                     AGGDIYNDDLKSVKFNNAEGLKAANFINDMKKYIPEDALSKDSNEMIESVFGPGKAAF
                     TIMLSSAATSTFDKSFPDLNWGYVTSLEDKTVGSFASVDDLTLMSACKNKELGFKLLE
                     YMLSKESMEAFHKEIPRAPVAKGEKYQGDPRFEEMVTRDKHMYRPLTVGPHGVEIYEY
                     LWKQLQMMIAGDITPEQALNESAKYANGLLAQ"
     gene            complement(23748..24905)
                     /gene="ftsZ"
                     /locus_tag="ACGI59_000959"
     CDS             complement(23748..24905)
                     /gene="ftsZ"
                     /locus_tag="ACGI59_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFG9738763.1"
                     /translation="MLNFDVELEECAQIKVIGVGGGGNNAVNRMVEAQLKGVEFISVN
                     TDKQALYTSKAEYKVQIGEKLTRGLGAGANPEVGKRAAEESKDEIVKLLQGADMVFVT
                     AGMGGGTGTGAAPVVAGLAKEMGILTVGVVTKPFAFEGKIRMKNAEGGIAELKSKVDT
                     LITIPNDRLLQIVQKNTSMLDAFAVADDVLKQGIQSISDLIAVEGLINLDFADVTTIM
                     KDKGLAHMGIGSASGETRAIDAARQAIQSPLLETSIQGAKGVLLNVTGGPNLGLFEVN
                     EASTLVMESCDPEANVIFGASIKEDLGDEIMITVIATGFEGLQNGALDLDTKPKSSIR
                     SSLNTTVKQAVKEIEEEVIAEEKIEPPKKASIIEEDDDESMEIPTFLRRRR"
     gene            complement(25095..25448)
                     /locus_tag="ACGI59_000960"
     CDS             complement(25095..25448)
                     /locus_tag="ACGI59_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small basic family protein"
                     /protein_id="HFG9738764.1"
                     /translation="MLWVLGGLAFGVVVGYYIPFTYSMDYSIYMSVAILAIMDSIFGA
                     IKSNFEDNYDNVIFITGFIGNAILAILLTYIGEKLGVSLYYVAVLVFGMRLFNNLSII
                     RRYIISNIMNKKRKN"
     gene            complement(25547..26221)
                     /locus_tag="ACGI59_000961"
     CDS             complement(25547..26221)
                     /locus_tag="ACGI59_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HFG9738765.1"
                     /translation="MKKKIVILGAFILGVFISTYIKSLDPNRVYITLGQTESIESEVE
                     NTKKEIKNLKETLKNNKKTLNEYKEAKKNGNESIQTLMQKELEEVKELSGYSTVTGPG
                     ITITMRDSERELKDGQNPNDLIIHDIDILRVLNDLKKAGARAISINGERVLATSKIKC
                     SGATITVNDTTYGQPFIIKAIGDIDLLSAAINSPESYAKSLKDIYGINIKVQENNGNK
                     VDLFAK"
     gene            complement(26226..26924)
                     /locus_tag="ACGI59_000962"
     CDS             complement(26226..26924)
                     /locus_tag="ACGI59_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HFG9738766.1"
                     /translation="MKKRFINKSIIIGSIVLGVLVSLQLKIINIENRGMTTFKRGEQL
                     TEELKSLKKEKENLQSEVNSVKKDIETYRGKNNEGEQDILKSEIEKYETLAGYTDVEG
                     TGIKIEINSTKDLGNSDKNDSILYNYDLLLSMINKLNSAQASAISINDQRIVSNTYMY
                     LKEDKLYMNDTVIKEPIIIKAIGDSDTLASALNIKYGIVWEIETYYNAKVKVTKEDNI
                     EINGHSDINKAEGK"
     gene            complement(26947..27687)
                     /locus_tag="ACGI59_000963"
     CDS             complement(26947..27687)
                     /locus_tag="ACGI59_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HFG9738767.1"
                     /translation="MKKRRKLNTNNVMIVLVFILMLSFCICIIVKSDLFDVKKIDVIG
                     NKRVTKSNIMKELNVNLNENIFAYNFKDMKNKLIKNPYIENVEIKRKLPNKIIISLKE
                     KEIFAVLKDEDNYCYIDKKGNLLEELRGSNESKKDLIVDVDYSIDDNKSIKFKNYKTK
                     ENVFKTLNYLKEEGIYRKINYVNLKKESNIEMLTRSNIKILLSNDDNLDYNISRVSKI
                     LIDLQNKNTNGGTINLNYGKLAVYSPEG"
     gene            complement(27957..29183)
                     /gene="murG"
                     /locus_tag="ACGI59_000964"
     CDS             complement(27957..29183)
                     /gene="murG"
                     /locus_tag="ACGI59_000964"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFG9738768.1"
                     /translation="MKVLLSGGGTGGHVYPAIAIANKIRDEHPDAEIIFVGTEKGIES
                     EIVPKYGFELKTVTVQGFKRKIDFDNVKRVFKLFKGLEQSRKIVKKFKPDIVIGTGGY
                     VSGPVLFNASMGKIPAIIHEQNSFPGVTNKILSKTVTKVLTSFEDSHKRFPEAAEDKL
                     VFTGNPVRKEILLSRKNIARKNLSISDEKRMVLCYGGSGGSRKINDAMRLVIKNMVNE
                     DIAFIFATGKSYYDEFMGSISDINLKPYQKVVPYLEDMANALAASDLVIGSAGAISLA
                     EITALGKPSIIIPKAYTAENHQEYNAKSIEKQGAGIAILEKNLTPESLNTAVFKLLGD
                     RELLVDMANASKTIGKPEAIDLIYDEIMKVYNSTQKSTSKKTKKEKVIKEVKEIKKET
                     TPSIEGQAKVIGIKKR"
     gene            complement(29201..30331)
                     /gene="spoVE"
                     /locus_tag="ACGI59_000965"
     CDS             complement(29201..30331)
                     /gene="spoVE"
                     /locus_tag="ACGI59_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein E"
                     /protein_id="HFG9738769.1"
                     /translation="MPKENLKKQIDIRVKTEFDGVVFYTTMLLVFVGIVMVFSASFIQ
                     SSFKHNDAYYFLKKNVIYAVLGFIVMIITSRIDYSFWKKNATAIGAIAVVLLLLVLTP
                     LGIEANGAKRWLGIGALTFQPAEIAKFATIILTAKLIEKNYDKIKSLTKGVVPLLVVP
                     GIFFALIILQPNLSTAGTVILVTFVMIFVAGMDMKIVFAMIGSGAALFAALVIAEPYR
                     LSRVTSFLDPFQDPLGKGYQVIQGLYALGSGGLFGLGLGKSKQKYFYIPEPQNDFIFA
                     IIGEELGLIGCIIVIMLFVVLVYRCVRIALKTSNVFACMVVIGIGAQIGIQAALNIAV
                     ATSSMPATGVALPFISYGGTSLTIFMGAVGIVLNISKHVKIN"
     gene            complement(30383..31738)
                     /gene="murD"
                     /locus_tag="ACGI59_000966"
     CDS             complement(30383..31738)
                     /gene="murD"
                     /locus_tag="ACGI59_000966"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFG9738770.1"
                     /translation="MDLKGKKVLLVGLAKTGISTIKHLDKLGASIIVNDIKDENKLRN
                     ILDELKSINDIKYILGHHPEDVDDIDMVVVSPGVPLDLPFILKLKNSGKYIIGEVELA
                     FKLSDNPIFIGITGTNGKTTTTSLVGEIFSRAKRDTYVVGNIGNPVIDTIETSSEESV
                     LVTELSSFQLESIDEFRPKVSAILNITEDHLNRHHTMEKYIEAKANIFMNQTVEDFCV
                     LNYDDEIVKSLADKCNAKVIYFSRTKKVNGGVYLENNDIIIDIDDKIKFLNKDDVSLP
                     GGHNLENCMAAIAIAYVCKIDLEVIKDVLMTFKGVEHRQEFVRNLDNVIYVNDSKGTN
                     PDSTIKAIQSYDRPIILIAGGMDKGSNFDELLETAKSYVKSLVLLGETASNIENCAKN
                     KGFNDIHIVKDMEEAVKTSYEISKSGDIVLLSPACASWDMYESFEVRGKDFKDNVNNL
                     K"
     gene            complement(31757..32725)
                     /gene="mraY"
                     /locus_tag="ACGI59_000967"
     CDS             complement(31757..32725)
                     /gene="mraY"
                     /locus_tag="ACGI59_000967"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFG9738771.1"
                     /translation="MMLGITELTYTALIAFLIVIIIGPIFIPMLRKFKFGQTVRDDGP
                     QTHLAKNGTPTMGGIIMIVAILITGLTRVKVSHDMAVGLICIAGFGFIGFLDDFIKIK
                     LKRSLGLKAYQKIILQVALSFYVAFYQYTSSSSASQLMIPFTDFVINVGILYIPIMMF
                     IIVAIVNAVNLTDGLDGLASGVTLIVSVFFMLFASSIAGNTEVAVLAAATVGACLGFL
                     GFNSYPARVFMGDTGSMALGGAVVAFSVLTNSVLIIPIIGGIYFAEALSVLIQVGYFK
                     ATRKRFFKMAPIHHHFEQCGWPETRVVFIFWIITVVLAWISIIAVF"
     gene            complement(32766..34139)
                     /gene="murF"
                     /locus_tag="ACGI59_000968"
     CDS             complement(32766..34139)
                     /gene="murF"
                     /locus_tag="ACGI59_000968"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFG9738772.1"
                     /translation="MECLTIKELVLATNGKLIYGDYNDCVSDIVIDSREANAQNAFVA
                     IVGENLDGHTFMKSAYDSGCKTFIKNESNGIKLESSDINLIEVKDTSLALGDISKYYK
                     EKFDIPFIGVTGSVGKTTTRDMIYSAISAKLNILKNEKNLNNHFGVPLTLFNLNKEHE
                     CAVIEMGMSGFNEIKYLVDIVNPKIAVISNIGLSHVEKLGSQEGILKAKMEITSNFDE
                     TNTLIVNGDDKFLSTLKEKEHVYKLKTFGFNKNNDVYCESYTMEEDSLTFICVIDGKK
                     EEIFIPTIGEHNIYNAMSAILVGLCLNISLDYIKKGLKNFKGTKMRLDIIKNEKLTII
                     NDSYNASPDSMDAALKILGRYKGRRVAILGDILEMGEISEYGHRLVGKSSMNNTDIII
                     TIGENSVFIGEEAKQLGFNSANIYHFENREDVFNKLNELVKTGDTILVKGSRGMRLEK
                     IVEYLNK"
     gene            complement(34306..36285)
                     /locus_tag="ACGI59_000969"
     CDS             complement(34306..36285)
                     /locus_tag="ACGI59_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein D"
                     /protein_id="HFG9738773.1"
                     /translation="MRKVKRISKKRLVLVLILACALFFCLVIRTGYLQLMKGNWLSTK
                     ALEQQTRDIPIEPKRGTIYDRNMKELAVSVTKYTVWCKPVEVEDKKEAAEKVAEILDE
                     DYKDIYALISKKNMALVKVKRWIDDDKASQIRDAKLSGIWVAEDNQRYYPYGNFAPYV
                     LGHTSSDATGISGVEMQYDKKLKGKPGKLIVSTDASGREIPQGMEKYYEPVQGNGLVL
                     SIDEVIQHYTEKAVQKAYELNNAKKVTAIAMNPKTGDILALASKPDYDPNDSRTPIYP
                     YYQEELEKYNDKDKIKGYYQMWRNPAVSDTYEPGSTFKLITSSSALEEGVIKDGEKFT
                     CTGSVTVGGRKIKCWRHYRPHGTQEFKQAVQNSCNPVFVELGSRLGVGKMYDYIESFG
                     LMDKTGIDLPGEAKGILYNEKNVGPVELATISFGQSISVTPIQLITAISSIANGGDLM
                     QPRVVKSYTDNKGNITETVKPKKVRSVISKETSKKMLEIAESVVTEGGGKIAYIPGYR
                     LGGKTGTAQKVIDGKYAPGKYICSFVGIAPCDDPQIVVLAIVDEPTGVSAFGSTTAGP
                     IVKEIMNDSLKYLGVKPVYKEEEKAEYEKKQVKVPDVRNLKIGDAVKALEDAKLKPDL
                     DADIELPEDTKVKDIFPKPGVKVNEDSSITLYFEN"
     gene            complement(36297..36644)
                     /locus_tag="ACGI59_000970"
     CDS             complement(36297..36644)
                     /locus_tag="ACGI59_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9738774.1"
                     /translation="MKKNYKIHNLNEYKNKKKNNYKKRNKKKIGAKCILLIATFSVII
                     LNLCGYSKISEMKYDIYYLQKDLRKKEIVLEELKAKETEKTSIKEIELKAKELLNMDY
                     PKDGQIKYIDVDN"
     gene            complement(37125..38060)
                     /gene="rsmH"
                     /locus_tag="ACGI59_000971"
     CDS             complement(37125..38060)
                     /gene="rsmH"
                     /locus_tag="ACGI59_000971"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFG9738775.1"
                     /translation="MEFHHVSVLLNECIENLNIKPDGVYVDCTMGGAGHSKEIVKKLS
                     DKGLFIGFDQDKNAISTAKERLSEYESRVKFVHSNFENIKEELEKIGVYKIDGVLADL
                     GVSSHQLDEADRGFSYMQDAPLDMRMDVRCEFSAYDVVNTYTEDELTKIIKDYGEDNW
                     AKRIAKFIVEERANKPIETTGELVDVIKKAIPKKARIDGPHPAKRTFQAIRIEVNNEL
                     GVITKMINDASSIMNEGGRICIITFHSLEDRIVKNAFKHLASDCICPQHLPICQCDKE
                     SEVKIITRKPILPSEEEIEVNPRSRSAKLRVAEKI"
     gene            complement(38090..38836)
                     /gene="lgt"
                     /locus_tag="ACGI59_000972"
     CDS             complement(38090..38836)
                     /gene="lgt"
                     /locus_tag="ACGI59_000972"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFG9738776.1"
                     /translation="MDRVAFTLFGIDIMWYGILMACGMILGTLIAIKEAKRVGIKEDD
                     VLNIAIIAIPVGLICARIYYVVFNWSYYAQNMSQIFNFRGGGLAIHGGLIGGILAGYI
                     YTKIKNINFLKMADTVILGMPLAQAIGRWGNFINGEAHGGATNLPWGIMVDGVKVHPT
                     FLYESIWDFGIFIVLLLFRKNKKYEGQVIVTYITLYSIGRFFIEGLRTDSLMLGPLRM
                     AQVISLIGVIGGIIAHVYLSKKNKNNISEE"
     gene            complement(39230..40516)
                     /locus_tag="ACGI59_000973"
     CDS             complement(39230..40516)
                     /locus_tag="ACGI59_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFG9738777.1"
                     /translation="MEKIVNDILKEEVYYEKLQNGLDVYFMPKRGFMKKYAILATNYG
                     SNDLEFVPIGEDKKIRVNEGIAHFLEHKMFEQPDGGDAFDKFSKLGVNANAFTNFTMT
                     AYLFSATENFYESLEHLIDYVQTPYFTDENVEKEKGIIAQEIKMYNDDPDWNVYFNCL
                     KAMYVNYPARIDIAGTVDSIYKITKEELYKCYNTFYNPGNMALFVVGDLDVEKVIDVT
                     KKSNNYKVDRLSKSIERFYPEEPESVKEKEVIEKFPISMPMFNIGFKDSNVGLKGKEL
                     LRKEIVTDILVGMLFKKGSKLYEDLYMQGLINENFGAGFSSQVDYAFSIIAGDSKEPK
                     KVKEIILDYIEKSKKEGLSKEEFERTKKKKIGSFIKCFDSINFIGNSFISYVFKDINL
                     LDYLDIIKDITFEEVEERLKEHFKEEYCVISIVEPK"
     gene            complement(40531..41796)
                     /locus_tag="ACGI59_000974"
     CDS             complement(40531..41796)
                     /locus_tag="ACGI59_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFG9738778.1"
                     /translation="MVNTKKIINLGNNINLTLIKTEKFKSNLISLYVQRLLDEKETTK
                     NALIPSIIASGSAKYPSARAISNKLDDLYGSSMGADAVKRGERQVLSFKVINISEKYL
                     DESIFEEVVEFFNEVINNTLVVDGGFKEEYLNIEKENLREKIQSIINDKKEYAQDKCI
                     EAMCKGERYSVSEFGYEDEIDSITSRELYEHYKNILKTSPIDIVVEGNFDEDKVVDII
                     SKNLKFEREEIINIPRADFIKNVDEVKVIDEQMEITQGKLVMGYRANVDYADIDKYYA
                     LVVGSNVLGGGPHSKLFVNVREKESLCYYIFSSIEKYKSIMFISSGIETKDYDKAVEL
                     IKKQVESVKAGDISDEELENSKLALVNSMKSITDNIGGMSDFVFSQSMAKTNSEVQDI
                     ISSIEKVTKKDIVEAIKNIELDTVYFLRN"
     gene            42112..43209
                     /gene="ychF"
                     /locus_tag="ACGI59_000975"
     CDS             42112..43209
                     /gene="ychF"
                     /locus_tag="ACGI59_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFG9738779.1"
                     /translation="MKLGIVGLPNVGKSTLFNAITQAGAESANYPFCTIDPNVGVVSV
                     PDERLNKLQELYNSEKIVPTAIEFCDIAGLVRGASKGEGLGNKFLSHIREVDAIVHVV
                     RCFEDENVVHVDGSVDPLRDIETINLELIFSDIEILERRINKTQKAAKADKTLGSELD
                     LLKSIMSTLEESKCVRTMEFTEDEQTFVNSLDLLTSKPVIYASNVSEDDLADNGENNK
                     YVQQVKAFAETEDAEVVVVCAQIEAEISELDSAEEKKEFLETLGLEQSGLDKLIKSSY
                     ALLGLISFLTAGPKEVRAWTIKVGSKAPQAGGKIHSDIERGFIRAETIAFNDLVEHGT
                     MAAAKEKGLVKLEGKEYIVKDGDVILFRFNV"
BASE COUNT        13536 a         7965 c         4546 g        17309 t
ORIGIN      
        1 tctaaaaatt atttttttaa ttttatgaat ttaattatac tttttctagt atcaggattt
       61 acaattaata tagaaccaat cataattggt gttagtatac aaaataaaaa aataatatct
      121 ttttttagat tatattcttt agattctaat ttataattat tatttattat tccatcataa
      181 tcacttatat ttgacttatt tttatttgta tcttgatttt tgttagaaat actttcccta
      241 taaggaaaag aatgttcatt ggtaccaaat actaattttg agcttaaatc ataattatca
      301 tcattttctg taattgaatt tttattgtaa aatccttttt ctttatcaga attattttct
      361 ttattaccgt ctttaggtat atcatgagga tttttatttt cattgctatc agtacttgat
      421 tctacatcat ctttaggagt atttggttgt ttctcatcag aattatcatt ttcattatta
      481 ttattttcat tattattgtt ttgattgtta ttactatcat tattttcatt tccattattc
      541 ccatcttgat tgttatcatc ctcatctcta tccgggtcgt ctctacctcc tcctccacgg
      601 tcaccatcaa tatcatcatt ttcccgttca tcatctggtg tctcttggtt gtctccgcct
      661 cccattataa atcctggttt aaaaactata tttgaatacc cttcttttaa tcctcctacc
      721 acttttactc taaaacaacg caacttaaaa tcttcaaaaa aaagtatagc ttgatctcta
      781 atatcctcct gttcttcaga tatccaattg attccatcgc ttgaatattc aacaaaaacc
      841 ttacttgcac tatacggcat atcataaaat aaaacagcta catatccatt ttttgtattt
      901 tgaaattcca gttccaaatt gtctattggt tttttctcta taacagatac attcaaaata
      961 ggaactacat catcatttat tataattttt tctttattta acatttctgt tttaaacttt
     1021 cctgttatat taaaattttc tcccttttcc aacttttcat aataatcagt aaaatcccat
     1081 tctacatcta tatcaagtcg attaccacta gatgtttcta ataccatata tttgggaaat
     1141 tttatatttt ctattttatc atctttactt acttttattt catcactaaa tacaccaatt
     1201 cccctatgac aacatttcac aatatcatag ccagaatctt cagctataga gggtataagc
     1261 tcattataat catcaattat ataaactttc ttactttctg atataattga ttgtgccaac
     1321 ttattattat cttttagtaa cccactttca ttgttgctac tatatgcttt tattgaagtt
     1381 tcttctatct caacatcacc tttagaataa acacctatag catgttctcc attcacttca
     1441 atatccttat ttagtattct aatatcattt tcagtataaa ttcctattgc atctttccca
     1501 tttgccttta ttttttcaac agattcgtag gatgatttaa tatgtaagtc tcctccttct
     1561 tcaacgtata aggctgtccc atcagtagca attatgttgt ttataccaat acttatactt
     1621 ccaccatttt ctatatgtat tactccttct tttcctcctt cacctattat attcaattca
     1681 ttatcatcaa taataaatct cccatgattt tttatcaata ttttatattt gttggtatct
     1741 attgtaaaat tactgtcata aggaatataa aatctaaact cttcatctat taccatatca
     1801 caagaaagag tataaactat ttcttcttta ttatttttat tttcttgaac ccacctttca
     1861 aaatcttcaa gacaattttc ctcttgtcca aaaactttat gtggaaaact tataaaacac
     1921 aaaactatta tactcataaa aataaatatc tcatactttt tcatcttaca ctcctagaaa
     1981 ttttttagct ttgaaacaat ctgtcgaaac atacacttca cccactataa tttttccaca
     2041 attatcaatt attgtcaatt tataaaagta tctttattaa ttgttataaa taaaaagcta
     2101 cttactaaag taaacagacg tttcacttta ataagtagct atatttttat aacaaaatag
     2161 ttataatatt agaacttcct tcatttacaa ttttttgtaa agtcttttgc attttaactc
     2221 ttatctcttc tggcatagta tataatttac tttgtaattg ctcttttact aagtcatgta
     2281 gtgatttacc aaacatattt gattcccata aatctgctgg ttgctcttca aatacttcca
     2341 taagatattt tatcatctct tcaccttgat tttgatttcc aactattgga gaaacctctg
     2401 tagatatatc tgcttttata atatgaagtg atggtgcatt ggcttttaac ttgataccat
     2461 attgttttcc ttgtttcatt atttctggtt cttccagact aagttcttct aaggaaggag
     2521 ctactactcc atatcctttt atcttggcat ctattaaagc actttctatt ttatcatact
     2581 catttttaac tttagatagt ctagttatca gactaagcag ttgataatct ccttcaattt
     2641 taaatccact tttttcttca aggacattat agaataaatc ttgctttgtc tgtaaatcaa
     2701 tatttataac accttctcct agctctacat tatcaacacc tgtatcttcc aaaaactcta
     2761 actcagaaaa tccttgtata ataccttcta tatcccttat tttacctata tctataatac
     2821 tttgttttaa tgttgttatt atgctacttt ttatccaatg atttctttca agaccttcaa
     2881 cccattttgg taagttgatt cttatttctg taagtggaaa atcatataat acagcttcca
     2941 taacctcctc tatatcttct tcttccattt caaccacatt cattggaaga actggaactt
     3001 catacttttc ttccaattca cttctaagca tactcgtttc ctcacttttt ggacttaacg
     3061 tatttaaaac cactgcaaat ggttttttaa gatttttcaa ttcttgtata actctctctt
     3121 ctggttctac atagcttttt ctgtcaattc cagtaactga accatctgtc aagacaacta
     3181 tccctattgt agaatggtct tttataactt ttttagttcc tatctctgct gccttttcaa
     3241 atgtcatagc ttcttgtgac caaggagtgg atactaatct ttgttttcca ccttcctcat
     3301 gccctaaagc tccttcaact atgtatccaa cacaatctac cattcttact tttaaagata
     3361 ctgtgtcttt tatttttatt tctacaccat ctgctggtac aaattttggt tctactgtca
     3421 ttatcgtttt acctgaacca ctttggggta tctcatctct tgttctatcc tttttaaatt
     3481 cattgtctat attaggtata accaactttt ccataaattt tcttataaaa gttgattttc
     3541 ctgttcttac aggtccaact actccaatat atatatcccc ctgagtcctt ttggatatat
     3601 cttcgtatat gttattattc atattattcc tcctatacat ctaatcttat taaattatat
     3661 ttagagttaa gggttaatat tcttaaaata gaaaaataaa gaccagcaat attaatttaa
     3721 tttatatata gaaatttgta aatttcaatg taaattaaat taatattgct ggtccaaata
     3781 gtagtgtatt atattaactg actttagtag tattttcatt tgaattatta ttcatctcaa
     3841 taagttcttt acgtctttct tttgtcatgt tcatccatat aaatgtaaac accaatccaa
     3901 ttatggctac tatagtaaga actatgaagt agtacctata tccagttaca cccggaaatg
     3961 catccaatat cttccctgct gctaaaggac aaagtacttc tggaagatat cctaacgtag
     4021 caactattcc tatagcagtt cctgatattg ataatgaata attaccttct tcaagtaacg
     4081 aatagtgtaa accttgaatt gcatacatag atacatatat tgcaacacat ccaatcaata
     4141 gtatataaat catagatgat tttgttggtg taaatattac acctaaaaga cctgcaatca
     4201 taagtataaa tccataagat ataatctttg atgaaccaac tttatctcca agtaatccag
     4261 aagcagctga tgctactggt ctacaatact gagctaatat tgagattgca gatgcaaaca
     4321 ccacagtagc tccaaatgct tcagttgaat aaggcgtaaa ataataaaat gacatattag
     4381 ctgaatatga acaaaacatt attattgtta ttatccatgt atgaggcatt tttattactg
     4441 caaaaaaggc ttctttatca aattttgctg atacctcttt ttcattatcc tttaatttga
     4501 aaaatactaa tactccaact attacattta ttgctgagta taatactata atagctgtaa
     4561 gaccagattc atcacttact tttgatgaaa tatacccaaa tagtatcaat actagagata
     4621 gatgaattgc attggtaatt ccacgaccac tttccataaa tccaaaagct tttccttgtt
     4681 cattttcaga tgctaacatt ctaacagctt ttacaagagc tggccaaaaa gtaagtattg
     4741 ttgttattcc ccataaggca tggattaaaa acacaacaat atatggagga taaagtgtta
     4801 atacaagacc agatattcct gtaataatta aagaacctga aagcaatttt ctggctgaaa
     4861 tacggtctgc taaaaaacca ccaaaaaggt atgataccat tgcaagtaga ccataaatgc
     4921 ttcctaagct tcccatttgt gtatttgtaa gattaaatgc ttgtgcaaaa gtatcataat
     4981 aataattctt taaatatggt aaagcatata taaatgctcc tgctacaaag agtattatca
     5041 agactttgaa atttttccaa atgcttggtt tatttacttc aactgacata cttcctcctt
     5101 atgtaaatac ctgtaaattt tcataataat taattattgc ttattttatt gtttattcaa
     5161 tatttaggaa tatttattaa tatattaggt taatctatca ataatttatg gtgagttatg
     5221 agctaaaagt aaactctttt agctcaccat aataattata tttttattta tactaacatc
     5281 cagagttatt agatatcttt ttttcatagt tcttccatag cattaaacct gttattataa
     5341 caaaaagacc tcctcctatc atttcaatct catagattat attatctttt aaagtctcca
     5401 taactggtgc cgttgagaaa agtatgccaa ctacagttac tacaagtacc attctggcaa
     5461 tattgataca aactgaatca ctttttgcaa ttgaatatgg tctttcttca tttggtcttg
     5521 tctttctaag tctaatataa gatgtaaaaa gcaagacata tggaaataat gctgttaatg
     5581 ctgtcatagt aacaagaaca ttgtatatat tatctactga tggtaataag tttgttccta
     5641 caagaagtag actcactatt atagcctgaa ctattactgc atttgatggt atattgtatt
     5701 tatttgtaac agtcaattgc ttaggaaata caccttctct tacatttcca aaaagcatct
     5761 ttactggacc tgctatataa agtattaatg ctcctagtac tgataaagtt attccaaatg
     5821 ctactattct tataaaccaa cttcctatac ctaaatcttg tgcaacttta gctaatgcat
     5881 ctagtatccc tgtagatgct gcaattttat ctggaggaag tatcattgtt attgctactg
     5941 aacctaagat atagattcct cctattaaaa cagccacagt taatatagct ttaggaaagt
     6001 ttttcttagc attatccatt tcggttatga aattagcagt cgtttcagct cctgataatg
     6061 caaatattat agcagatata gaaactaatg aattggcatc tatttttggt attatatttt
     6121 ggattgtata tatcgatgct gatggagctt ttttgagaat aactacagac ataaatgcca
     6181 tcactataag taatatagca ggaattgtag aacctaatgc tcctgtattc gtaaaaaatt
     6241 ttccaaatgc catcccacga gtacttacta aggacaacag ccaaaatatt atgagtgaaa
     6301 gtgctaaaat aaacatctta ttgtttgata aatctggatt tccaagcata tatgcaatat
     6361 ttatcgctaa aaaagtcaag aaagaagaat accaaaatat ctttgatgtc caatttagcc
     6421 atgacaccat aaatccccac ttctctccat aagcttgttt aacccattct ccaagtccac
     6481 catcttttgg atatgttgca gccaactctg cacatataag tgatgcagga acaaagaata
     6541 taaaagcaaa caataaccaa actggtatag aaccaagacc caatccaaaa ctagaggcag
     6601 tagatttagc tatccatcta atcccaaata gtgctgatat attcataaat actaaatccc
     6661 aaaatccaat tttctttttc actgtaattc ctccaatttc aaatttattc agactcatac
     6721 acaatgtatt gacttaaata tcaagtataa gttcttcaac acttcttgga taattagtta
     6781 taattgtagc accattctta cctactattg ctgtatctga gtgtctaaat cctccaactc
     6841 caggtatata tatccctggc tccatagaaa ataccattcc ttcttctaat attaattcat
     6901 tgtcaaatct taaatatggc tcttcatgtt ctgataaacc taaaccatga ccaattctat
     6961 gatttgaata taactctaga ccatactttg caacaacagc tcttgctgct tcatccacca
     7021 ttcttgctgg tataccagct tttattgtat ctaatccagc ttgttgagcc tctactgcaa
     7081 tcttaaacac ttctttttgt ctttctgttg gtttgcctat aataaaagtt ctttcatttt
     7141 ctgctctata gttttcatac caaacctgtc tactatgtat tactatatct cctctttgaa
     7201 gtattctagt attggaatct aaatgaggtt gtgctgttct atcaatccct gaacaagtcc
     7261 agtttgcaaa tcctatatat gtatcaggat aattttcaga aacatatttt agtaaagcat
     7321 tatcccctgc aacgtcaaac tccaattcac tcatcccaac tctaacattt tctaaagacc
     7381 cttttattcc tatatcagat aggtaacctg ctatcttcaa aaattctatt tcttttgcat
     7441 cttttacata tctcatttca aatattttac ttccaacatc tttgatagca aatgacttat
     7501 cagttatata ctctgaaaag cttgcagata ctatatcttt ttctattcca acagttgttc
     7561 ctttagggta tttatttaaa attatatcta agtaatgttt atgtgatatt ccatgttctt
     7621 tcatttctgg tatttcataa tacacatata cattatcaac ttttgcaact tctttagcat
     7681 gtaattcttc taatccaggt actattaatt ctattttatc ttgagttact actgttacaa
     7741 ttggtctaga ataagttatt gctttaaatc cactaatata aaattgattg tctggttcaa
     7801 atattaaagc aatttcaatg cttttttcat tcatgtactt tctcaatctt tctagtctca
     7861 tattatttga cataatgcac ctctttctta atattcagat aatttattca atacaacaag
     7921 gatgaatata ttattttaaa attcttaata tttttagtac ttgtcgtatt tgtatgtaaa
     7981 ttaattttat ttgtgattat tttatcataa tcatgattat tttgtcaatc atgattttat
     8041 tttaaaaaat tttttatatt ctagtgattt taaattatct aaaatataac gagtatacat
     8101 tgtattttgt aatattattc ttgatttatg gtaagtttaa tgataaaatc acgattatat
     8161 aaactatatt gattatttgt aggtgaataa aatgacaaat aaagaattac aaaagaaaag
     8221 gatattgatg tatttcatag aagctgctca aaatattatg gaaaacgaag gtatagaaaa
     8281 tattacactt cgtaaggttg cagatatggc tggctataat agttcaactc tatataaata
     8341 ctttaaaaat ttagaccatt taatatcatt tgcatctata aaatatttta aagattataa
     8401 cttgaatatt tcaagatgta tagaaaatgt caatgatgaa tataagaaat atataattat
     8461 gtggaaatta ttttgtaagc attcttttga taatgctcaa gctttttacc aggtattttt
     8521 taacttgtct agtgatgaac ttagctatat aacaaaacaa tattatgata tgtttccaga
     8581 agatttaggt attcatgata gtgacatatc tttaatgatt acaggagaaa gtataaagga
     8641 aagaaataag atactattaa acaatcttgt agatactgga tatataccag ctaaagattt
     8701 agacataatc aatgatataa ttatttcttt ttatcaaaat ctattattct taaaaaagaa
     8761 cacaggaaaa aattctgatg atgatgaatt aactatgaaa atgatttctt ctattgaata
     8821 cataataaaa catgctaaat aaaattaaaa tatggctgtc tcaaaataga gataagcctt
     8881 ttttattgtt gaaataataa aaatatattt aatttcatat aaaaaagagt gcctcgtaaa
     8941 ctaaaaagtt cattttgaga cacccctaaa ataaatttat tatttattat acaatagttt
     9001 atatatcatc acctaaaact acttcttcca tttcatgctt tttacttctc atcattagtt
     9061 ctattccaac ttctttagga tttgaacctt tctttattac agaatatata gcattagtta
     9121 taggcatatc tatatctaat ttttcagcaa catcatgtgc tacttcagta gcagttatgc
     9181 cttctacaac catctttact tcttttaaag tatcttctaa actcatccct ttacctatga
     9241 gtataccagc tcttctattt ctactatgca tacttgtaca agtaactatt aaatctccaa
     9301 tacctgaaag acctgcaaag gtagacatat tagctcccat agcaattcca agtctgctca
     9361 tttcactaat acctcttgtc ataagggcag cttttgcatt atcaccatac cctaatccgt
     9421 cacatatccc agctccaaat gctataatat tttttaatgc tcctccaagt tctactccaa
     9481 ctatgtctgg attcgtataa actctaagtt tagggctcat aaataaatct tgtatcatct
     9541 gtgctatttt taaatcttta gaagcaacta ctacggtagt aggaatatcc cttgctactt
     9601 cttcagcatg agatggccca gatagtatta catatggatt ttgtggtaat tcttcttcac
     9661 agacttgaga taatctaagc ccagtccctt tttcaagacc tttagctaca ttaacaataa
     9721 cttgtccttc ttttataaat ggttttattt tttggcaagt acttcttaca gcctgtgatg
     9781 gaactgctaa aactattacc ttacaatctt ttatagtttt ttctatatca gtacttatag
     9841 ttatattatt tggaaataat actcctggta aatagtctgt attttcttta gttctgttta
     9901 tttttttagc ttgttcctct tttcgagtcc acatgctcac atcattacct ttttttgcaa
     9961 gccctaaagc taatgcactt ccccagcttc cagtacctaa tacacatact ttctccatgt
    10021 ttaacaccac ctgagtataa tatatttact attctttctt ttgaccaatt tttctctctg
    10081 tcccatttaa taatcgcttt atatttgctc tgtgattata tattactgat gcagttaaga
    10141 acagagctac tattaaacca gctttatttt ttaccataat cataaatatt ggtgaacatc
    10201 ctattcctac aacagaaccc aatgaaacat actttgttat agctaccacc aatataaata
    10261 ctgctagaca cattagtgtt attactggat ttacagctag cattgcccct agtgaagttg
    10321 caacaccttt gcctcctcta aatcctaaaa ccgctggcca attatgacct gctacaacac
    10381 atataactgc cagatatcca gctaaaagag tatctattcc aaccaatctt gctgcaaatt
    10441 ctgatattaa aacagccatt acacctttaa gaacatcacc taaaaatgtc attgctcctg
    10501 ctctttttcc aagtgtccta agtacatttg tcgaacctgc attaccagaa ccttgagtcc
    10561 ttatatcaac cccagcaatt cgctttgcaa ctatgtatga ggtagatata tttcctaaaa
    10621 gatatgcaac tacagcaata attatataac taaatatctc cactgaattt ccccccttta
    10681 gttatatttt aagatattta ttttttattt ttttgtctat actcaaactt tatggaagtt
    10741 ccttcaaatc caaaattttc tcttatctta ttctcaaggt atctttgata tgagaagtga
    10801 gttaaatcct tatcatttat aaacaatgta attttaggag gtctaatgtc agtttgagtc
    10861 ccatagtaaa tttttaatct tctacctttg tcagatggtg gttgatttaa cattacagct
    10921 tctccaatta cttcattcag tgctgaagtt gatattctct tagaatgttc atttgacaca
    10981 tattcaactg tatctagtat cttgttcatt ctttgattag tttttgcaga aacaaatacg
    11041 attggagcat acatcataaa tggaaatttt tcttttatat cttttgtgta attgctcata
    11101 gtcttattat ctttctctat taaatcccat ttattgatta caaatatgca tccttttcct
    11161 tcatcatgtg ctatacctgc aacctttgta tcttgttctg ttacaccttc taaggcatct
    11221 attactatca atactacatc tgctctatct acagcactca tagcccttat tacactgtat
    11281 ttttctattg tctcatatat tttacttttt cttctaagcc ctgctgtatc tattagaaga
    11341 aatttctgtc catttttttc aaaataagta tcaattgcgt ctctagtagt tcctgcaatt
    11401 ggacttacaa ttactctttc ttctccaagt atcttattga gtatagaact ttttcctgca
    11461 tttggtttac ctgttatagc tactcttatt atatcttctt catactctgt atctaatcct
    11521 gctggaaaat tttcaactat ctcatctaat aaatctccaa atcccatact attggctcct
    11581 gaaactgcaa aaggcgttcc aaatcccaac tcataaaaat cgtatatatt atcaaattga
    11641 ctttgactat caatcttatt aaccactaag attactggtt tttttgtctt tctaagcaat
    11701 tgtgcaattt ctttatcagc agctgttatt ccagctttac catcaactat aaaaagtatt
    11761 acatgtgaca tgtccattgc aagcatggct tgatttctca tttgagataa tattatatct
    11821 tcgctatcag gttcaattcc tcctgtatcc actaatgtaa aatatttatc taaccattct
    11881 acttcagcaa atattctatc tcttgtaact ccaggtgtat tttcaactat tgatatcctt
    11941 tttcctgcaa atttattaaa tattgtagac ttaccaacgt ttggtctccc aactacagct
    12001 acaactggcc tacttatact cattttattt ctccttccat cttttgtttt ctattttaaa
    12061 attttatcca caaaatcttt tccttcattc aaacaaggga taatttttat tctcataagt
    12121 tcttctaatt gatttaattc aatattatct aagaaaattt cttcatctgc ttttagcata
    12181 cttcttgtta tataaagagc ttctcctaat tctttatctt caagttgttc ttttaaatcc
    12241 ttagctgtta aaagacctga tacagttatt gtctctccaa aaaacttgtt tttgatttca
    12301 tatacattta cttgaagatt tttaaacttg tccatcatag catctgccat actctgtata
    12361 aattcatatg cagaatgacc agtagctatt gatactttct tctctctttt tagaatcttc
    12421 tcaggcaata tatttagata ctcttcaatc tctgttttta acttacgtat catacctaca
    12481 ccatcttcaa actgtaaaaa accttcatat tcttcatatc caggcaattc actattagat
    12541 agtatataaa attcatctga taaaaatgca aatcgagtac ctaatttttc aagatatttc
    12601 tgttgtattt gatgtatttg ctctatagtt ttacttgcgc tcttatcatt aaatatattt
    12661 aattctacta gattttctct atgctttgta atcccaactg gaactatcgc aacactgttt
    12721 acatacggat aaagttgagc tagttctttt attgttctat ccaattcctt tccatcattt
    12781 atacctggac ataaaacaat ttgacagttc atttctatat gagcatctgc caatctcttc
    12841 attataccat ataattttcc tgcaaattta ttgcttatca ttttttgtct taactctgga
    12901 ttagttgtat gcacagatat atttatagga cttattctat attttataat attatttata
    12961 tcttcttcac tcatatttgt aagtgtgaca aaatttcctt gtaaaaaaga taatctcgaa
    13021 tcatcatctt taaaataaag agtttctctc ataccttctg gcaattggtc tataaaacaa
    13081 aacatacact tatttctaca gcttttagct tggtcaatta tcggatttgt aaattcaaca
    13141 cccaaatctt catcatagtc tttttctatc tcgtatatgt agacttcgcc atcttttttt
    13201 tgtatttcta catctaaata ttcatcactt agtaagaatc tatactctat tatatcatgt
    13261 attggctgtt cattcacaga tataagtaaa tctcctacct ctatacccat ttcttctgca
    13321 atactatctt tataaacctt acttataata ttatttactt tttttacatt tacttccaat
    13381 attttcacca ctcttttgta ttcatcaatg cttattataa tgtattttaa tatgcaattt
    13441 atttatttat tatattttat gatagcattt ttaagcattt catgtcataa taacacattt
    13501 taattctaat agtcaatata agtaaatagc tataaaatag attagaacaa ataatattca
    13561 aatttaagtt tcttaaaaat ttaaatatta tttgttctta aaaaaatttt ataaatttaa
    13621 tgttttacta tgattaacgt cccatttgtc atatcatgaa ccattccacc tacacacttt
    13681 gacatcaaca atgccttatt ttgtaagtct ttctcatcat ttgcataaaa tattggacaa
    13741 cctccaccat ttttcatatt cttatcaagt gtaactatag ctagggtata ttcatttaaa
    13801 cctatatcca ttcccatctc atgcacttcc tttctttcct attttataat tttttagtga
    13861 taagtttttt cctttagagc ttgaaagtat tgggcatgac tttacagctt ccattacttt
    13921 ttccacattc ttatctactg gtaaataagc aatcattata ctttcatcct ctttatttct
    13981 tctaggtaat ggtacgaatg aaggttctcc ataatctcca tataaaccta accttgaata
    14041 tatattgtat actattgctt gtctttgacc ggagtcaaat aatattcctg cattagcata
    14101 atccttattt ttaggaatta tttgtattcc tacacctcta ttaatatata cttctctatc
    14161 gctttttaga cctatatttg ttataccttt taaatctccc acctttagta tagattcgtc
    14221 cacaaaacta atttcagcag gcattacatc agctatgtct ccaatacttt ttctagtaag
    14281 aagttttttt aatataaagg ctaacactaa tccacaaatt gaacttatta ccaaactcat
    14341 cccttcactt attttcgttt ctgcaagtat tacataatac aatcctattg ttaaaaaaga
    14401 tgtaactata cacatataat ttcttacttc ataggttcta gctatctctt ctatgaatgc
    14461 agctcctctc tttacaagtt gaacttcctc taaattttgc aatgtatttc ttctatttgc
    14521 tctcacttgt ctaaattgtt ctgctgctag agataaaaaa gttatagatg tataagaacg
    14581 atttataaga gctggaacca ataatgctcc taatgaagaa gcgacgaatg ctaatataag
    14641 ttgagatagt aaaacatttg gttgcgttgg atactgtttt tggtctaaat tcaacattat
    14701 aattctagat gttataccaa taataacagc aacaaaaaat actctatcca ttaaatcact
    14761 cctaaaatat ttaatttgaa attttacatg taaatctaca aagtctttct agaaaaatca
    14821 ctctgctttc cttttgatgt ttctatgata ggtgtacttt tcactacatc tatcatgaca
    14881 tctatatctt tgagtattgg catatatggt ataaccacag tacttttttc taaattggtc
    14941 ttagatatag caacaatatc aacctcatct acatctctat ctatccccat atgaataaat
    15001 agattatgta ttatagcttg tctttgccct atatcattta ctattccaaa tgcacctaaa
    15061 tctcttggta taacttctat ggccagacat tcatttctat acttttctct tgtactttct
    15121 agacctatgt tagttattat tacaccattt accattaaaa taggtccttc aaaacttatt
    15181 tttgcaggaa ctatatctgc tatatctgct atactgttcc ttcttaaaaa tcttctaaaa
    15241 atcaaaccaa caatagctcc tgacacaata gctaggattg tgcaataaaa aaagctaagg
    15301 ccatactttc ttgcaaaaac gatataaact atcgaagata ccagtgcaga aaaaagactt
    15361 atataacttc tagattcata cgtagatgct atttcttcaa cgtaagcgtc tccttttggt
    15421 actaattctt cattatcgat attctttagt gttattcttt cttgttctgc aagaccttga
    15481 aactgttgta ttgctactgc aaaaaatgtc aaggctgcaa actccttatc tataagtgca
    15541 ggtaatgcta tagcacctaa cgaagctgaa agcccagata taattatctg ttctaaataa
    15601 tcttgtggtc ttgatgggta ttgtttgtct gtaactctta aaactaatcc tctacataat
    15661 attcctacaa ctaatgcaac tataaaagag tgtctaaata aatttgcatt caataaactt
    15721 tcttccattt tctcagctcc ttctcttata tagtttgtgc aaaaaacaaa aaaaattccc
    15781 ttctatttaa ggaaattttt ttgtaatatt gtaactataa attcgctacc aatgccaagt
    15841 tcacttttta cttttataca tccatttagt gatttgacta tatgctttgt tatagccaat
    15901 cctaaacccg ttcctcctac atccctactt cttgctttat ctaccctata aaatcgttca
    15961 aatatgcgtt ctacatcttc ttttggtatc cctacaccat tgtctataac tttaattacg
    16021 attttttctt catcttttaa cactacaact tgtacaatcc cattatcttg ggtatacttt
    16081 atagcattat caactaggtt taagaacact tgctttatat agtctttatt ggatagtata
    16141 cttattgatt tatcatcaca tttatataaa acttctatat ttttttcact agctgaatgt
    16201 tgagttattt catatacctc tttaaataca tcatatacta caacattttc cataagaaga
    16261 gtatctttat tttctacaaa tgataacagc aatatatcat caatcaacct ttttaatcta
    16321 tctgattcgc tttctattat ttctaagaat ctatttctag tattcacatc tatattttca
    16381 tttgacttta gtgtttcaac aaacccactt atagatgtaa gtggtgtttt aagttcatga
    16441 cttacatttg caacaaaatc acttctcata ttttcaagtt taactttttt agtaatatct
    16501 tctatattta ctatcgaacc tattattacg ttgttagtat tctgaagata aactgggtct
    16561 acttttatct tatatacgat atcatcttta gtagtaatat tagcagtttc attatcttta
    16621 gaaccaataa attgaagtat ttgttttaat atttcttctt cttttattac aacattaata
    16681 ttatgacctt caactaattg atttggttca ctctttatca tttcttttgc ttcatcattt
    16741 atcagaagta tatttccatt tacatctata gccaaaattc catgagatat actctttaat
    16801 atagacctta actgtaaatg tttatactgt acttcttcta aggttttatc catattcttt
    16861 atcatatgat taaaatttct agctaattcg ccaagttctc ctttggctga tatgttaagc
    16921 ctagcatgat attcattatt acttactttt tttgagacgt atataaactc ttttaaatat
    16981 ttccttaatg ttattgtgta tcttacagct aatataacaa tggctatgga caacataaca
    17041 ataaaaaaat atatactatt taaatcttcc acaagataac actcctaaaa tataaccttt
    17101 ttaatctatt ttgtatccta cacctcttat agtctctata tatttttccg atttatcttc
    17161 atcttctatt ttttttctta aatatcttat atgaacatct actgttctag tttctccata
    17221 gtattcatac ccccatattt tatccaaaag ataatttctt gaaagtactt ttcctttatt
    17281 ttgaattaat aattttaaca attcaaattc cttaagagtt aactctatct tttcagagcc
    17341 tttggtaact tcatgttttg ataaatctag ttttaaatta cctgtagtaa gtatattgtt
    17401 ttcttcacct aagctagtca aattatatct ccttaaaaca gaactaatcc tagctaacaa
    17461 ctcttttata ctaaatggct tagttatata gtcatcagca cctatttcaa gcccttcaac
    17521 cttgtcattt tccatatttt tagcagtaag cattataaca ggaatatttt ttaagtcctt
    17581 atcacttctt atttttctca aaacttctat tccacttata tttggtaaca tccaatctaa
    17641 aagtattaaa tctggtttaa tttccttagc ctttataaat ccgtcaaatc catcatatga
    17701 ataactaact tcgtaatttg acacctccaa attgaatttt aatagctcaa ctatatgcat
    17761 ttcatcgtct ataacaagca ctttagtatt cattaaaaag ctcctctcta ttttatctgt
    17821 agtaccgttc ctattcgttc tgatgcctta ttatgttttc tataagagtg gaatttatct
    17881 gctctacaac tagtgcataa atttaaattt attatatttt cttttttaac tccactacat
    17941 ctaagcatat actcattaat tttccataaa tctaaatagt aacttccctc ttttattata
    18001 tagaactttt catcattatt tgtaagaatt gtgttaaatt tttctactaa atcttcagac
    18061 acttcataac aacatggtcc aattgatggc ccaattatac atactaaatc ttcaggaact
    18121 gttttatagc attcagacat taattttatt gttttatgac ctatattact aaaagtccct
    18181 ctccaacctg catgagccag tcctatggct ttatttttag catctataat tgatataggt
    18241 acacagtctg ctgtaaacag taaaagtggt actttttcca agtttgttat aagtgcatct
    18301 ccatctcttc ttttccctat agtatcttta ttgactatat taactatatc tgaatgtatt
    18361 tgagaattac tagttagatt ttcaaatgtc aaatcacatt ctttacatac ttttaacata
    18421 tcttctctat tttttgcatc tatattctta catgttataa ctagatttac agaatttaat
    18481 ttttcatcaa gttcttcaca attaccactt aaattaacat actctttaat atcttgtaca
    18541 ttttttatat ttatataatc tttcatcatt tgtctccttt ttctatcagt atacttttaa
    18601 gttcatttac aaaagtattt atatctttaa attgtctata cacagaagca aatcttacat
    18661 aggcaacctc atctaactct ttcaatttat ccattaccat ctcaccgatt tttttggttt
    18721 caatttcttc tataaaacat ttatttattt cattttcaat atgtacaaca atactttcaa
    18781 tctcgtcaat agaaacaggt cttttttcac atgcttttag caatccatat tttatctttt
    18841 ctctatcaaa atattctcta ctattatcct ttttaatgac cattaaagga gttgtttcta
    18901 ttttctcata cgtagtaaac ctctttttac atgattcaca ctcacgtctt cttctgatag
    18961 attttaaatc tgtatgtcta gaatctatta cctttgattc tttataatta caataaggac
    19021 attgcatcta tattcctcct ttgtttaact atatctcaaa ctcatctaag cttaattctg
    19081 aacctatatt tactattatt gtatcacaac ctattttaag tatatcattc caaaatataa
    19141 cttcttcatc ccctcctctt gaaaaaaaac ttctacttcc atctccaaaa atggtaaatg
    19201 agactacctt accttcgtga atatccatgt ctatgtctat tataaacccc atcctttttc
    19261 cattttttac atttataatt tctttttcca ttatatcaga aactctaatc atattgtact
    19321 caccctttct ttaaaaataa atatgtatcg aagtaaaaaa aaatgaccta tataggtcat
    19381 ttaaattaaa ataatgttta cagtccatgg attacttata catattttct catatttttt
    19441 aaagcatttt tttcaattct tgatacttgt gcttgagata taccgatttc atcagcaact
    19501 tctatctgag ttcttccttt gtaaaacctt aaatctaata ccaacttttc tctactattt
    19561 aatttcttaa tagcttcttt aagtgatatt tcttgcaacc aattttcatc agtatctttt
    19621 ttatcttgaa cctgatccat aacgaatatt gcatctccat tgtcttgata tactgggtca
    19681 aacagagata ttgggtcttg tatggcatca agtgccatta caactgattc tacttctaat
    19741 tctaattcct ttgctatttc tgatactgta ggttctttag aatttgttct aattaatctt
    19801 tctctaactt gtagtgcctt atatgctata tcttttaatg acctacttac tctaattggg
    19861 ttattgtctc ttagatacct tctaatttct cctataatca taggaacagc ataagttgaa
    19921 aaccttacat tttggcttaa atcaaagtta tctatagctt ttataagacc aatacaacct
    19981 atttgaaata aatcgtctat attttctcct ctgttgttaa acttttttat tacactcaag
    20041 actaatctta aattccctct tacaaactgt tgtctggctt cttcatcacc attttttatt
    20101 tgtaataaaa gttccttcat ttgcttattt ttaagaactg gaagttctga tgtattaaca
    20161 ccacatattt caaccttatt aacttgcata tattttcagt cctctctcaa aagtattcat
    20221 ctattgaaat tatttacatt tccaattttt ttatactttt taagaagaat taatttagca
    20281 ttatacaaat tttttcattt ctttttgcaa ccttgagatt attttctttt caagtctaga
    20341 tatgtaggac tgtgatattc caagcattcc agcaacttct ttttgtgttt tttcaatacc
    20401 tttatcaata agaccaaatc ttaattccat aatttgcttt tctctgtcac ttaatctgtc
    20461 aagtgccata accaacaaat ctctatctat ttcttcttca attattttgt atatttcatc
    20521 attttctgta cccaatacat cagataataa aagttcgttt ccatctaaat ctatatttaa
    20581 aggttcatca aatgatactt ctgttttctt tttattattt tttcttaagt acattaatat
    20641 ttcattttct atacatctcg aagcataagt tgccaatttt atatttttat tcagtttaaa
    20701 agtattaact gcttttatca aacctatagt tcctattgaa atcaaatctt ctacatctat
    20761 acctgtattt tcaaattttc tcgatatgta gactactaat ctaagatttc tttctattaa
    20821 aattgacttt acactttcat ctgtttctag cttttgtaaa agctccattt cttcttctgg
    20881 tttcaatgga ggaggaagta tatttgcccc tcccatatag tatattccct ttggctttat
    20941 tatttttaat ttaataccta acatagtaat aaagcttatt attctttctt tcaatcgtaa
    21001 cattccttgt cccccttaaa ttttcaccta aaatccaggc aatatatttg gattcattat
    21061 tgctccatat tctctatcat taaaatttga aataccaagt attatatttc ctatcttttg
    21121 ctcatctact tctatatagt cagcttttaa gcctattatt atgcttgtct tattacttcc
    21181 agcatgttta taaggtatta ttctaactct acctgaagtt ttatcatcta aacttcctat
    21241 aatttcttgt gctttcttca tatctatatc ttcataatta atatttaata gtttttctgg
    21301 aataaatcct tctaacacac tagacttaac tattattaca tcactttgac ttattgggtc
    21361 ttttaacaaa ttaccactat ctactaaagc cttacagcaa aaactttttt ctagtaagtt
    21421 tatatttata gtctttgtaa gctccttaat gtacttaagc attttcaaat ctttataaat
    21481 gtattttaat aattcacaac tcacataagt acatgcaatt aagaaagata tttttaagtg
    21541 acttattcct gtaaaataaa ttataaaata agtgcttcca gaaataaaaa tgtttactag
    21601 ataaaaaact aacgctacat gaacataatc ttttttgctc ttacttctaa atgaaattaa
    21661 agttattata ctcatgatta ttattttaaa tggtacagta aatagtatat tcattgatgg
    21721 atacaaataa gcaacagaat aggcacctcc taaactagcc cccaataact tatttttttt
    21781 aggattatat cttttagtaa gaatagaagt acaacttata ataatataat ttattactag
    21841 gttttctata acatagtact caatatacac catgtatccc cccttaaaac ctttaaacat
    21901 attataagat agtatgtaca aaattttatg tcacttttga tggtcgaatc caaaacaaat
    21961 tctatttaaa aagaacaatg atatatgtct tatactttat gtcgtcaaaa gatatttttt
    22021 taataccaaa caaatagact tttttgtaga atccaatata tctaggtaga agttattcac
    22081 tctacttttg gtatatttac aaaaaaagac tacataattt aataatatat caagtaaatc
    22141 ttttacttat ataaaatcaa attatatagt ctgtaaattc aaatataaat tattgtgcta
    22201 gtaatccatt tgcatatttt gcagattcat ttagtgcttg ttctggagtt atgtctccag
    22261 ctatcatcat ttgaagttgt ttccataaat attcatatat ctctactcca tgaggtccaa
    22321 ctgtcaatgg tctatacata tgtttatctc ttgtcaccat ttcttcaaat cttgggtctc
    22381 cttggtattt ttcaccttta gcaactggag ctctaggtat ttctttgtgg aaagcttcca
    22441 ttgattcttt gcttagcata tactcaagaa gtttaaatcc aagttcttta tttttacatg
    22501 ctgacattaa tgttaaatca tctactgaag caaaacttcc aactgtctta tcttctaatg
    22561 atgttacata tccccagttt aaatccggga atgatttatc aaaagtactt gtagcagcag
    22621 aggatagcat tatagtaaat gcagcttttc ctggtccaaa tacactttca atcatttcat
    22681 tactatcttt tgataaagca tcttctggaa tatacttttt catatcattt ataaagtttg
    22741 cagctttcaa gccttctgca ttattaaatt taactgattt taaatcatca ttatatatat
    22801 ctccaccagc ttgccataaa tacggatacc agttccagtt taagcttcca aatactttag
    22861 aaccccaacc ttgtgcaaga ccccactggt cgatttttcc atctccatca gtatcttttg
    22921 ttgctttttc acagattctt ttaaaatcat cccatgtttt tggaggtttt tctcctaatt
    22981 tttctagtat atctttattg tagtacaata cgcctggatt agcagcttct ataggtaaac
    23041 cataaatacc acccatcatt tttgcatcat ctaagtatag ataattatct gaagttcctg
    23101 atttttctaa ataaggtgtt aaatcttcta ccgcacccat ttctataaat tgaggaaaca
    23161 tctctgcata catgtatcct atatctggac cttcacctgc acttattgct gttgcatatt
    23221 tttctgcgta gttatcccat ggtactatct gaaattcaat ttcacagtta ttttcttttt
    23281 caaatttttc aaatatcggc ttccatgcat ctgcatcatg gtctgttaat ggtgcagacc
    23341 atacaactaa tttttcttta ccctctttgc ctttagaatc ttcttttttt gaacatcctg
    23401 caagaattga tactgataga atccctacca taactaaaga aagaaccttt tttagtttaa
    23461 tcatgcttaa tcccctttca gactttaatt tgtctttttc ctcaaatgtt tacatttaat
    23521 tcagatttaa tattaacaaa taagattact cttgtctaat cataaaaaac tatactaact
    23581 cctataacta tatttttttt ctatacctat tagatttaat tcatactaaa aacttacttt
    23641 ttaaatttta tcaatattta aaaatttttt taagtctagc cacaaactaa ctatctaaat
    23701 tgagcaaaat aaaaagaagt ctattgactt ctttataagc ttacatatta tcttcttctt
    23761 cttaggaatg taggtatctc catgctctca tcatcatctt cttcaattat acttgccttt
    23821 ttaggaggct ctattttttc ctcagcaatt acttcttctt caatttcttt tacagcttgt
    23881 tttactgttg tatttaatga agaacgtatg cttgatttag gtttagtatc taaatctaag
    23941 gcaccatttt gtaatccttc aaatcctgtc gcgattactg taatcattat ttcatcacct
    24001 aaatcttctt ttatagaagc tccaaatata acatttgctt ctgggtcaca ggattccata
    24061 actaaagttg atgcctcatt aacttcaaat aatcctaagt taggaccacc tgtaacatta
    24121 agaagtactc ctttagctcc ttgtattgat gtttctagaa gtggagattg aatagcttgt
    24181 cttgcagcat caattgctct agtttcacca ctagcactac ctatacccat atgagcaagt
    24241 cccttgtctt tcattatagt agtaacatct gcaaagtcca agtttattaa cccttcaact
    24301 gctataaggt ctgaaattga ttgtatacct tgttttaata catcatctgc aactgcaaaa
    24361 gcatctaaca ttgatgtatt tttttgtact atctgtaaaa gtctatcatt tggtatcgtt
    24421 ataagagtat ctacttttga tttaagttct gctattccac cttcagcgtt cttcattctt
    24481 atcttacctt caaatgcaaa aggtttggta actacaccta ctgtaagtat ccccatttct
    24541 tttgccaagc ctgcaaccac tggagcagct cctgtaccag taccaccgcc cattccggct
    24601 gttacaaaga ccatatcagc tccttgaagt aactttacta tttcatcttt actctcctca
    24661 gcagctcttt tccctacttc tggatttgct cctgctccaa gtcctctagt taatttttct
    24721 cctatttgaa ctttatattc agcttttgat gtatataaag cttgtttatc tgtatttact
    24781 gatataaatt caacgccttt tagttgagct tctaccattc tattgacagc attgtttcca
    24841 ccgccaccta cacctattac tttaatttgt gcacattctt ctaattctac gtcaaagttt
    24901 agcatcataa acccccttct ctcatgattc aaagttatcg tatgtattaa ttatatcatt
    24961 ttcttctttt attttgttaa cctttaagta acatattcca caaaaacgta aattttcctt
    25021 tttaaatttc ttacttatat tgtcaaatat tactctttaa gtataaaata atttacgtat
    25081 atattaaatg ctctttaatt tttacgtttt ttgttcataa tattactaat aatatatcgt
    25141 ctaattatag ataagttatt aaacaatctc ataccaaaaa ctaatacagc aacatagtaa
    25201 agagaaactc ctaacttttc tcctatataa gtcaataata tagccaatat agcattacct
    25261 atgaaaccag taataaaaat tacattatca tagttatctt caaaattgga ttttatcgct
    25321 ccaaaaatag aatccattat tgcaagaata gctactgaca tatatatgga ataatccata
    25381 gaatatgtaa atggaatata gtaacctact actacaccaa atgctaatcc tcctaaaacc
    25441 cataacataa atcttcacct ctttatttta ctttgaaaca caactacatt acaatccttt
    25501 ttattaattt tttttacttt ttctttattt ttttgtattt ttcgttttat tttgcaaata
    25561 aatccacttt atttccatta ttttcctgaa cttttatatt aattccatat atatccttaa
    25621 gagatttagc atatgattct ggagagttta tagctgcact caataaatct atatctccta
    25681 tagcttttat tataaatggt tgtccatatg tagtatcatt tactgtaata gtagctcctg
    25741 aacattttat cttacttgtt gctagtactc tttctccatt tatagaaatt gctcttgctc
    25801 cagccttttt taaatcattt aaaaccctca aaatatctat atcatgtata atcaaatcgt
    25861 ttggattctg tccgtctttt aactctctct cactatccct catagtaata gttatgcctg
    25921 gccctgtaac agttgaataa ccacttaatt ctttaacttc ttcaagctct ttttgcataa
    25981 gtgtttggat agattcattt ccattctttt ttgcttcttt atattcattc aaagtttttt
    26041 tgttgttttt aagagtttct tttaaatttt ttatttcttt ttttgtattt tctacctcac
    26101 tttcaatact ttcagtttga cctaatgtta tgtatactct atttgggtct agtgatttta
    26161 tataagttga tatgaaaacg cctaatatga atgctcctaa tataactatt ttctttttca
    26221 tactattatt tcccctctgc tttgtttata tcactatgac catttatttc tatattgtct
    26281 tctttggtca cttttacttt ggcattataa tatgtttcta tttcccagac aataccatac
    26341 tttatattaa gtgccgaagc caatgtatca gaatcaccaa ttgcttttat tataattggt
    26401 tcttttatga cagtgtcatt catataaagc ttgtcttctt ttaaatacat atatgtatta
    26461 gaaactattc tctggtcgtt tatagatatg gcacttgctt gtgctgaatt taatttattt
    26521 atcattgaca ataataaatc ataattatat agtatactat catttttatc agagtttcct
    26581 aagtcttttg tagaatttat ctcaattttt atgcctgttc cttcaacatc tgtatatcca
    26641 gctaatgttt catatttttc tatttctgac ttaagtatgt cttgttctcc ttcattgttt
    26701 ttgcctctat aagtctctat atctttcttc acactattaa cttctgattg tagattctct
    26761 ttttcttttt ttaaagattt aagttcttct gtaagttgct ctcctctttt aaaagttgtc
    26821 atccctctat tttctatatt aataatttta agttgtaaac taacaagaac acccaataca
    26881 atacttccta tgattatact tttatttata aatctttttt tcaatatatc acttccttac
    26941 aattgtctat ccttctggac tatacactgc caactttcca tagttaagat ttatagtccc
    27001 tccattagta tttttatttt gcaggtcaat cagaatttta cttaccctag atatattata
    27061 atctaagtta tcatcattag aaagtaagat ttttatatta cttcttgtaa gcatttctat
    27121 attgctttct ttttttaagt ttacataatt tatttttcta taaattcctt cttcttttaa
    27181 ataatttaaa gttttaaata cattctcctt agttttataa tttttaaatt ttatagattt
    27241 attatcatca atgctataat ctacatctac tatcaagtct tttttacttt cattagaacc
    27301 tcttagttct tccaataaat ttcctttttt atctatataa caataattat cttcatcttt
    27361 taatactgca aatatttctt tttcttttaa acttataatt atcttattgg gaagttttct
    27421 ttttatttcc acattttcta tatatggatt ttttataagt ttatttttca tatctttaaa
    27481 attgtatgca aaaatatttt catttagatt tacatttagt tctttcatta tatttgattt
    27541 ggtcaccctc ttatttccaa ttacatctat tttctttaca tcaaataaat cactttttac
    27601 aattatacat atacaaaaag ataacataag tataaaaact aaaactatca tgacattatt
    27661 cgtatttaac tttcttcttt ttttcaaata taatcaaccc ctttggttaa taaatacacc
    27721 tttagagctt gtttttccta caaaaaaatt ataatattta aagtcaaatt tttgcatttt
    27781 attacataac atccaaataa ggtattaaat tttataaata aatcaatatt tcatcaaaat
    27841 aaacatagca tactacatac tatttatata tttaaatctg tatacataca aataaacccc
    27901 taatttataa taatttagaa aatctaatat attataaatt aggggtaaat atttacttat
    27961 cttttcttta ttcctattac cttagcttga ccttctatac ttggagtcgt ttcttttttt
    28021 atttctttta cttccttaat aactttttct ttttttgttt ttttagaagt tgacttttgt
    28081 gttgaattgt atactttcat tatctcatca tatataaggt ctatggcttc aggcttacct
    28141 atagttttac ttgcatttgc catatcaacc aaaagttctc tatctcctaa aagtttaaat
    28201 actgctgtat ttaggctctc tggagttaga tttttttcta aaatagcaat tcctgcccct
    28261 tgtttttcta tactcttggc attatactct tgatgatttt cagcagtata agctttaggt
    28321 attattatag atggctttcc aagagccgtt atctcagcta atgaaattgc tcctgcgcta
    28381 cctataacca aatcacttgc tgcaagtgca tttgccatat cttctaagta aggcacaact
    28441 ttttggtatg gtttaagatt tatatcgcta atacttccca taaattcatc ataatagctt
    28501 ttgcctgtag caaatataaa agctatatct tcatttacca tattctttat tacaagtctc
    28561 attgcatcat ttatttttct tgaacctcca ctaccaccat agcaaagtac cattcttttt
    28621 tcgtcactta ttgatagatt ctttcttgca atattttttc ttgataataa aatttctttt
    28681 ctaactggat ttcctgtaaa aactaattta tcttctgctg cctctggaaa tcttttatgc
    28741 gaatcttcaa aacttgtaag tacctttgta actgttttag ataaaatctt attagttaca
    28801 ccaggaaaag aattttgctc atgtattatg gctggtattt tccccataga tgcattaaat
    28861 aaaacaggtc cactaacata tccaccagtt cctataacta tatctggttt aaatttctta
    28921 acaatctttc ttgattgctc caaaccttta aaaagcttaa aaactctttt tacattatcg
    28981 aaatctattt ttcgcttaaa accttgaact gttacagtct ttaattcaaa tccatactta
    29041 ggaactattt ctgactctat tcccttttcc gtcccaacaa aaattatctc agcgtctgga
    29101 tgctcatctc ttattttgtt tgcaatagct atagctggat acacgtgccc tccagttcca
    29161 ccgcctgata ataaaacttt catcaaatca tcatctcctg ttaattaatt ttgacatgct
    29221 ttgagatgtt taacactatt cctacagctc ccataaagat ggtgagtgaa gttcctccat
    29281 aacttataaa tggtagtgct acacctgtag ctggcattga tgatgtcgca actgctatat
    29341 ttaaagctgc ttgtattcca atttgtgcac ctatacctat aacaaccata caagcaaata
    29401 cattagaagt cttcaaagct attcttacac atctgtagac taaaactaca aatagcataa
    29461 taactattat acaaccaatc aatcctaact cttctcctat tatagcaaat ataaagtcat
    29521 tttgtggctc tggtatataa aaatactttt gtttactctt tcctaagcct agaccaaaca
    29581 atccacctga acctagtgca tatagacctt gtattacctg atacccctta ccaagagggt
    29641 cttgaaaagg gtctaaaaat gatgtaacac gactaagtct atagggttca gcaattacta
    29701 atgcagcaaa taaagcagcc ccactcccta tcatagcaaa tacaattttc atatccattc
    29761 cagcaacaaa tatcattaca aaagtaacta gaattacagt acctgctgtt gacaaatttg
    29821 gctgaagtat tatcaacgca aaaaatatcc ctggaactac gagaagtggt acaacacctt
    29881 ttgtcaaaga ttttatttta tcataatttt tttcaattaa ttttgcagtt aatattattg
    29941 tagcaaactt tgcgatttct gcaggttgaa atgttaaagc tcctattccc aaccatcttt
    30001 ttgctccgtt tgcttctatc cctaaagggg tcaaaactaa caataataat actactgcta
    30061 tagctccaat tgctgtagca tttttcttcc aaaaactata atctattctt gatgtaataa
    30121 tcataactat aaatccaagt acagcatata ttacattctt ttttagaaaa tagtacgcat
    30181 cattgtgttt aaatgaagat tgtataaaac ttgcactaaa caccataact attcctacaa
    30241 atactaaaag catagtagtg taaaacacta ctccatcaaa ttcagtcttc acccttatgt
    30301 caatttgttt tttcaaattt tctttaggca tccaactaat ctacctccat tcttccattt
    30361 aagtaagttt agaggtaaga gattatttta aattgtttac attatctttg aagtcttttc
    30421 ctcttacttc aaaactttca tacatatccc aacttgcaca agctggtgaa agaagcacta
    30481 tatctccact ttttgaaatt tcataagaag tcttaactgc ttcttccata tcttttacta
    30541 tatgtatgtc attaaatcct ttatttttag cacaattttc tatatttgat gcagtttctc
    30601 ctaataagac caatgattta acatatgact ttgcagtttc aagaagttca tcaaaattac
    30661 ttcccttatc catcccccct gctataagta ttataggtct atcataagac tgtatagctt
    30721 ttatagtgga atctggatta gttccctttg aatcatttac atatattaca ttgtctaaat
    30781 ttcttacaaa ctcttgtctg tgttccactc ctttaaatgt cataagtaca tcctttataa
    30841 cttctaaatc aattttgcag acatatgcta ttgctatagc tgccatacaa ttttctaagt
    30901 tatgacctcc aggtaaactt acatcatctt tatttagaaa tttgatttta tcatctatat
    30961 ctattattat gtcattattt tctaaatata ctcctccatt tactttttta gttcttgaaa
    31021 agtaaattac tttggcattg catttatcag ctaaagattt tactatctca tcatcatagt
    31081 ttaatacaca gaaatcctca acagtttggt tcataaatat atttgccttt gcttctatgt
    31141 atttttccat agtatggtgt ctatttaaat ggtcctctgt aatatttaat attgcgctta
    31201 ctttaggtct aaattcatct atactctcaa gctgaaaact gcttagttca gttactaaca
    31261 cagactcttc acttgaagtt tctatagtat ctattacagg atttcctata ttaccaacta
    31321 catatgtatc tcgttttgct cttgaaaaaa tttctcctac caaactagtt gtagttgtct
    31381 ttccatttgt acctgtaatc cctataaaaa ttggattatc cgatagttta aaagctaatt
    31441 ctacctcacc aattatatac ttgcctgaat tttttaattt caatatgaat ggtaggtcta
    31501 gtggtacacc tggagacact acaaccatat ctatatcatc tacatcttct gggtgatgtc
    31561 ctaatatgta ttttatgtca tttatagatt tcaattcatc aagtatattt ctcaatttat
    31621 tttcatcttt tatatcattt acgattatac ttgcacccaa tttatctaaa tgtttaattg
    31681 tagatatacc agtttttgca agtcctacta ataaaacttt ttttcctttt aagtccattt
    31741 ttcattcccc ctagccctaa aatactgcta ttatacttat ccaagctaaa acaactgtta
    31801 taatccaaaa aataaataca actcttgttt ctggccaacc acactgttca aagtggtggt
    31861 gtattggagc catcttaaag aatctctttc ttgttgcctt aaaatatcca acttgaatca
    31921 aaactgatag agcctccgca aaataaattc ctccaattat aggtattata agcactgagt
    31981 tagttagaac tgaaaatgct acaactgcac ctcctaatgc cattgaacct gtgtcgccca
    32041 taaacactct agccggatat gaattaaatc ctaagaaacc taaacatgct ccaacagttg
    32101 ctgctgctaa aactgcgact tctgtatttc cagcaataga acttgcaaat agcataaaga
    32161 atacagaaac tattaatgtt actcctgatg ctaatccatc taatccatca gtcaagttta
    32221 cagcatttac tattgctacg attataaaca tcattattgg tatatataat attccaacat
    32281 ttatcacaaa atctgtaaat ggtatcataa gttgtgaagc acttgatgat gatgtatact
    32341 gataaaaagc aacataaaaa gataatgcaa cttgaaggat tattttttga tatgctttta
    32401 gtccaagtga tctttttagc tttattttta taaaatcatc taaaaagcca ataaaaccaa
    32461 aacctgcaat acatataaga cctactgcca tatcatgact aacttttact cttgtaagtc
    32521 cagttattag aattgcaaca atcattataa ttccacccat agtaggtgtt ccgtttttag
    32581 ctagatgtgt ttggggtcca tcatctctaa ctgtctgacc aaatttaaat tttctaagca
    32641 ttggtataaa tattggtcct attataatta ctattaaaaa cgcaatcaat gcggtgtacg
    32701 taagctctgt tattcctaac atcataaaac tcctctccct tgtcaattaa ctcattagtt
    32761 taagcttatt tatttagata ttcgactatt ttttctaatc tcatgcctct tgagcctttt
    32821 actaaaatag tgtctcctgt ttttactaat tcatttaact tgttaaacac atcttctcta
    32881 ttctcaaagt gatatatatt ggctgaattg aatcctaatt gttttgcttc ttctccaata
    32941 aacactgaat tttcacctat agttattata atatcagtat tattcataga tgattttcca
    33001 actaatctat gaccatactc tgaaatttca cccatttcta gtatatctcc aagtatcgct
    33061 acccttctac ctttatatct tccaagtatc tttaaagcag catccataga atctggacta
    33121 gcattatatg aatcattaat tattgttaat ttttcatttt ttattatgtc tagtctcatt
    33181 tttgtccctt taaaattttt taaacctttt tttatataat ctaaagaaat atttagacac
    33241 aaaccaacta atatagcaga cattgcatta tatatattat gttctcctat tgttggtata
    33301 aatatttcct cttttttccc atctattaca catataaatg ttaaactatc ctcttccata
    33361 gtataacttt cacaataaac atcattgttt ttattaaagc caaatgtttt taacttatat
    33421 acatgttctt tttcttttaa tgtagataaa aacttatcat ctccatttac aattaaagta
    33481 tttgtttcat caaagtttga agtaatctcc atctttgctt tcaaaatacc ctcttgtgaa
    33541 cctagttttt ctacatgtga taatcctata ttcgatatga ctgcaatttt aggatttact
    33601 atatccacta aatattttat ttcattgaat ccagacatac ccatctctat tacagcacac
    33661 tcatgttctt tatttagatt aaataaagtc aatggaactc caaaatgatt atttaagttt
    33721 ttttcatttt ttagtatatt taactttgca gatatggcag agtaaatcat atctctagta
    33781 gtagtttttc ccacactacc tgtaacacct ataaatggaa tatcaaattt ttctttatag
    33841 tattttgaaa tatctcctag agccaaagat gtatctttta cctctattaa atttatatcc
    33901 gaactttcta attttattcc attactttca ttttttataa aagttttaca tccactatca
    33961 tatgcagact tcataaatgt atgcccatct aaattttctc caactatggc tacaaatgca
    34021 ttttgggcat ttgcctccct gctatctata actatatcac ttacacagtc attataatct
    34081 ccatatatta acttcccatt agttgcaagt actaactctt ttattgttaa gcactccatt
    34141 attaaactcc tcctttaaaa ccttagctat attctcttag caacaaaagg caatgtatac
    34201 cgttatatat acattgccta cgataactct taactttata attatatact atatttctgt
    34261 ttttttgaag atgtttatgg gtatatgtta attatctgtt actttttagt tttcaaaata
    34321 tagggttata cttgagtctt cattaacttt tactccaggt tttgggaata tatcctttac
    34381 ctttgtatct tctggaagtt ctatatctgc atctaagtct ggttttaact tcgcatcctc
    34441 caatgccttt acagcatctc ctatttttag atttcttaca tctggcactt taacttgctt
    34501 cttttcatac tcagcttttt cttcttcttt atatactggt ttaactccca aatattttaa
    34561 agagtcattc attatttctt tcactattgg tcctgctgta gtactaccaa atgcacttac
    34621 tcctgttggt tcgtcaacaa tagcaagaac tactatttgc gggtcatcac aaggtgctat
    34681 tccaacaaat gaacatatat actttcctgg agcatactta ccatctataa ctttttgagc
    34741 tgtacctgtt ttaccaccaa gtctataacc tggtatatac gctatctttc ctccaccttc
    34801 agttacaact gattctgcaa tttctaacat ttttttagat gtttctttag atataacact
    34861 tcttactttt tttggtttta ctgtttcagt tatatttcct ttattgtcag tataagattt
    34921 taccactctt ggttgcatta aatctccacc attagctatt gatgatatag ctgttataag
    34981 ttgtattgga gttactgata tagattgacc aaatgatatt gtagccaatt caactggtcc
    35041 tacatttttt tcgttataca gtattccctt agcttctcct ggtaaatcta tacctgtctt
    35101 atccataaga ccaaatgatt ctatataatc atacattttt ccaacaccta atctgcttcc
    35161 taactcaaca aatactgggt tgcaagagtt ttgtactgct tgtttaaatt cttgtgttcc
    35221 atgaggtctg taatgtctcc aacatttaat ctttcttcct ccaacagtta cactacctgt
    35281 acatgtgaat ttttctccat ctttaattac tccttcttca agagcactgg aagatgtaat
    35341 aagcttaaat gtagaacctg gttcatatgt atcacttact gctggatttc tccacatttg
    35401 ataatagcct tttattttat ctttatcatt atatttttca agttcttctt gataatatgg
    35461 atatattggc gtccttgagt cattagggtc ataatctggt tttgatgcta aagcaagtat
    35521 atcacctgtt ttagggttca tagctattgc agtaaccttt tttgcattat ttaactcata
    35581 agctttctgt actgcttttt cagtatagtg ttgaattact tcatctatag acaacacaag
    35641 accattacct tgtacaggct cataatattt ttccattcct tgtggtattt ctcttcctga
    35701 agcatcagta cttactataa gttttccagg ctttcctttt aactttttat catattgcat
    35761 ttctacacca gatatacctg ttgcatctga agaagtatgt cctaacacat atggtgcaaa
    35821 attcccatat gggtaatatc tttgattatc ttctgccacc catattccac ttaatttagc
    35881 atctcttatt tgacttgcct tatcatcatc tatccatctt tttactttta caagagccat
    35941 attttttttg cttattaaag cgtaaatatc tttataatct tcatccaaaa tctcagctac
    36001 cttttcggct gcttcttttt tatcttctac ttccacaggt ttacaccaaa cagtatactt
    36061 agttacactt actgctaact ctttcatatt tctatcatat atagttcctc ttttgggttc
    36121 tattggtata tctcttgttt gttgttctag tgcttttgta ctcaaccaat ttcccttcat
    36181 aagttgtaga tacccagtcc taataacaag gcaaaaaaac aatgcacatg ctagaataag
    36241 aacgagtacc agccttttct tacttatcct ctttactttt ctcaaatcta ttccccctag
    36301 ttatccacat ctatatactt tatttgtcca tcttttggat agtccatatt gagcaattcc
    36361 ttagccttta gctcaatttc ttttattgat gttttttcag tttcttttgc ctttagttcc
    36421 tctaatacta tctctttttt tcttaaatct ttttgtaaat aatagatatc gtacttcatt
    36481 tccgagattt ttgaataacc acatagattc aatattatta ctgaaaaagt tgcaatcagt
    36541 aaaatgcatt tagctcctat ttttttctta tttctctttt tataattatt ctttttttta
    36601 tttttatatt catttagatt atgtatttta taattttttt tcaaattctc accccgatag
    36661 tatatagttt aaattttcct ctatactata tttgttatta atcatatttc ttcctctaaa
    36721 atttcatcgc taaattaata cactattaca caatctaatt gtattttttt ctatacacaa
    36781 caaaaaccta taaattcact tatactttta tatgaatcta taggttagat attaatttct
    36841 atttattttt atcacttcta gtatttttag ataatttctt ccataaatat aatatgtaac
    36901 taactatcta ctctctgatt cctaagttct ccattatatt acttaatacc atctatatct
    36961 atatttttat atctaaattt tcattatata tctcaataac aaagaacatt tcttatagtg
    37021 ttaattgttt tttaattgct ttaccagtgc aacaaaatat ataatacttt ttaattaatt
    37081 ataataatct tatatataag attattcttt agccttataa ctacttatat tttttctgct
    37141 actcttagct tagcacttct tgaacgtgga tttacttcta tttcttcctc actaggtaat
    37201 ataggttttc tagttattat tttcacctca gattctttat cacattggca tataggtaaa
    37261 tgctgtggac atatacaatc tgatgctaaa tgcttaaatg catttttcac tattctatct
    37321 tctaatgaat ggaatgttat tatacatatt cttccacctt cattcattat agaagaagca
    37381 tcatttatca tctttgtaat tactccaagt tcattattta cttctattcg tattgcctga
    37441 aaagttcttt tagctgggtg aggtccatct attctggctt tcttagggat agctttttta
    37501 attacatcta ctaattctcc tgtagtttct attggtttgt ttgctctttc ttcaactata
    37561 aatttagcta tacgcttagc ccagttatct tctccataat cttttattat cttagttaat
    37621 tcatcttctg tataagtatt tacaacatca tatgcagaaa actcacacct aacatccatt
    37681 ctcatatcaa gtggtgcatc ttgcatataa gaaaatcctc tatcagcttc atcaagttga
    37741 tgagatgaaa ctcctaaatc agctaataca ccatcaattt tgtaaactcc tattttttct
    37801 aattcctctt ttatattttc aaaattgctg tgaacaaact tcactctact ttcatattca
    37861 ctcaatctct cttttgctgt gcttatagca tttttatctt ggtcaaaccc tataaataat
    37921 cccttatctg acagtttttt tactatttct ttagaatgtc ctgctcctcc catagtacag
    37981 tcaacataca cgccatctgg ctttatattt aaattctcta tacactcatt taaaagaact
    38041 gatacatgat gaaattccat agttacctcc cttaaattca ctttttaaac tattcttcac
    38101 taatattatt tttatttttc tttgataaat aaacatgtgc aattatgcca ccaataactc
    38161 caattagact tataacttgt gccattctaa gtggtcctaa cattaaacta tctgttctta
    38221 atccctctat aaaaaatctt cctattgaat ataaagttat ataagtaact ataacttgac
    38281 cttcatattt tttatttttt ctaaatagta ataaaactat aaatattcca aaatcccata
    38341 tagactcata taaaaaagtt ggatgtacct ttactccatc aaccattatt ccccaaggta
    38401 aattggtagc acctccatgt gcttctccat taataaaatt tccccatctt ccaatagctt
    38461 gtgctaaagg cattccaagt ataactgtat cagccatttt taagaagtta atatttttaa
    38521 tcttagtgta tatataacca gctaatattc caccaattaa ccctccatgt atagcaagtc
    38581 caccacctct aaaattaaaa atttgagaca tattttgagc atagtaactc caattaaata
    38641 caacataata gattcttgca cagattagtc caactggaat tgctattata gctatattta
    38701 atacatcatc ttctttaatt cccactcttt ttgcttcttt tattgctatt aaagttccta
    38761 aaatcattcc acatgccata agtattccat accacatgat gtctatgcca aacagtgtaa
    38821 atgctactct atccataacg tcacctcttt gtattaattg aaaatatttc caaagcaata
    38881 tgctataatt acaattaatt gtaattatac taaattatga taacacatta atgttaattc
    38941 tacaaaaaat attaaaattt attctacaac tccctacact atattatatc taattactat
    39001 aaaatttttt aaactaaatc tctcatgact aaagttacaa gtgtacatag atatttttta
    39061 atcaaaaaga ttgatgcata tatacatcaa cctcttctaa ttcttatcta tagctcttaa
    39121 atttttacct caatttatat aaatatattt tataaatttg ttttaccaaa ataaacattt
    39181 aaaaacataa actaatactt aattagttat tcttattatg attaagtttc tatttaggct
    39241 ctacaataga aataacacaa tactcttctt taaaatgctc ttttaatctt tcttcaactt
    39301 cctcaaaagt aatatcttta ataatatcta aataatccaa tagatttata tccttaaaaa
    39361 cataagatat aaaactatta cctataaagt ttatagaatc aaagcactta ataaatgacc
    39421 ctattttttt ctttttagtt ctctcaaact cttcttttga taatccttct ttttttgatt
    39481 tttctatata atcaagaatt atttctttta cttttttagg ctctttagaa tctccagcta
    39541 ttatgctaaa agcataatcc acttgagacg aaaatccagc tccaaaattt tcatttatta
    39601 aaccttgcat atacaaatct tcataaagct tacttccttt tttaaataac atccctacaa
    39661 gtatatcagt tacaatctct ttacgtaaaa gttctttccc ttttaaacca acattgctat
    39721 ctttaaaacc tatgttgaac ataggcatag atatagggaa tttttctata acctcttttt
    39781 cttttacact ttctggttct tctggataaa atctttctat actcttagag agtctgtcta
    39841 ctttgtaatt gtttgacttt ttagttacat ctataacttt ttctacatct aagtcaccaa
    39901 caacaaataa agccatattt cctggattat agaaagtatt ataacattta tatagctctt
    39961 ccttagttat cttatatata ctatcaacag tacctgctat atcaattcta gcaggataat
    40021 ttacatacat tgccttcaag caattaaagt atacattcca atcagggtca tcattgtaca
    40081 tttttatctc ttgtgctatt ataccttttt ccttttcaac attttcatct gtaaaataag
    40141 gtgtttgaac atagtcaatt agatgttcta aactctcata aaaattttca gtagcagaaa
    40201 ataagtaggc tgtcattgta aaattagtaa atgcatttgc atttactcct aattttgaaa
    40261 atttatcaaa tgcatctcct ccatcaggtt gctcaaacat cttatgctct agaaaatgtg
    40321 caataccttc attcactcta attttcttat cttctccaat tggtacaaat tctaagtcat
    40381 ttgaaccata attagtagcc aaaatagcat actttttcat aaaacccctt ttaggcataa
    40441 aatatacatc taatccattt tgcaatttct cataataaac ttcttctttc aatatatcat
    40501 taactatttt ttccatagac atctcctcca ttaatttctc aagaagtata cagtatcaag
    40561 ctctatattt tttatagctt caactatatc tttttttgta accttttcta tactagatat
    40621 tatgtcttgt acttcagaat ttgtctttgc catagactga gagaaaacaa aatcagacat
    40681 accacctata ttatcagtta tagatttcat agaattaact aaagcaagtt tactgttttc
    40741 taattcttca tcagatatat cacctgcttt tacactttct acttgttttt ttatcaattc
    40801 tacagcttta tcataatctt tagtttctat accagatgat ataaacatga tacttttata
    40861 cttctctatt gaagagaata tatagtaaca taaactttct ttttctctta cattaacaaa
    40921 taatttagag tgtggaccac cacctaagac attgctccca actactaaag cataatattt
    40981 atctatgtct gcataatcca catttgctct ataacccata actaacttac cttgagttat
    41041 ttccatttgt tcatctataa cttttacctc atccacattt tttataaaat ctgctcttgg
    41101 tatatttata atttcttctc tttcaaattt tagattttta cttattatat ctacaacttt
    41161 atcttcatca aaattacctt ctacaactat atctataggt gatgttttaa gtatgttttt
    41221 ataatgttca tataactctc ttgaagttat actatctatt tcatcttcat aaccaaattc
    41281 acttacacta tatctctctc ctttacacat agcctcaata catttatctt gcgcatattc
    41341 ctttttatca tttattattg attgtatctt ttctcttaaa ttctcttttt caatatttaa
    41401 atattcttct ttaaacccac catctacaac aagcgtatta tttattactt cattaaaaaa
    41461 ttctactact tcttcaaata tactctcatc taaatacttt tcacttatgt taattacttt
    41521 aaaactcaat acttgacgtt ctccacgttt tacagcatca gctcccatag atgaaccata
    41581 taaatcatct aatttattgg atattgctct agcactagga tatttagcgc ttccacttgc
    41641 tattattgat ggtattagag cattttttgt agtttctttt tcatccaata gtctctgaac
    41701 atataaactt ataagattcg atttaaactt ttctgttttt atcaaagtaa gattaatatt
    41761 attacctaaa tttattattt ttttagtatt aaccatatgt acctcctatt tttatttaaa
    41821 catcaatttt aaaattgaaa tttatttatt ttttaattac atatactcac atataaaaac
    41881 cttctattta attatatcct ttttattatt ttctaaaagc aatattttta tttaaattaa
    41941 atacaaatat tatttaaatc aatatgcttt aaagtctagt ttacaagctt gatatatatg
    42001 atgtataatc aatatgtatg tctataatta aaacaatttt gcaatcctat agattgattg
    42061 tttaaagtta tataaagaat ataaaaaaga ataagagaga ggacgattaa tatgaaatta
    42121 ggtatagttg gtttaccaaa tgtaggtaaa agcacactat ttaacgcaat aactcaagca
    42181 ggtgcagaat ctgcaaatta ccctttttgt acaatagacc caaatgtggg agttgtatct
    42241 gttccagatg aaagattaaa caaactacaa gaattatata attctgaaaa aatagttcct
    42301 actgctatag aattttgtga tatagcaggt ttagttagag gagcttctaa aggagaaggt
    42361 ttaggaaata aattcttatc acatataaga gaagttgatg caatagttca tgttgttaga
    42421 tgttttgagg atgaaaatgt tgtacatgta gatggttctg tagacccact tcgtgatata
    42481 gaaactataa acttagaatt aattttctca gatatagaga ttcttgaaag aagaattaat
    42541 aaaactcaaa aagctgctaa ggctgataaa actttaggtt ctgaacttga tttattaaaa
    42601 tcaataatgt ctactttaga agaaagtaaa tgtgtaagaa ctatggaatt tacagaagat
    42661 gaacaaacat tcgtaaattc attggattta cttacatcta agcctgtaat atatgcttca
    42721 aatgtatcag aagatgattt agctgataat ggagaaaata ataaatatgt tcaacaagtt
    42781 aaagcatttg cagaaacaga agatgccgaa gttgttgtag tatgtgcaca aatagaagca
    42841 gaaatttctg aacttgattc tgctgaagaa aagaaagagt ttttagaaac attaggtctt
    42901 gaacaatcag ggcttgataa gcttataaaa tcttcttatg ctttacttgg tttaatttca
    42961 ttcttaactg ctggtccaaa agaagttaga gcatggacta taaaagttgg aagtaaagct
    43021 cctcaggcag gaggaaaaat acattctgat atagaaagag gatttatcag agcagaaaca
    43081 atagccttta atgatttggt tgagcatggt actatggctg ctgctaaaga aaaagggttg
    43141 gttaaattag aaggtaaaga atacatagtt aaagatggag atgtaatatt atttagattt
    43201 aatgtttaaa atgattaatc gtatacactt tgactattat atataataac taaaaaacta
    43261 agattataat aatacaatat tatcttataa taattatata gaatttaaaa ataaaagaag
    43321 agtatttcaa atgatttgta taaattatta tgaaaa
//
LOCUS       DAYAPA010000020        42163 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.020, whole genome shotgun sequence.
ACCESSION   DAYAPA010000020 DAYAPA010000000
VERSION     DAYAPA010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 42163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42163
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.020"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            292..1818
                     /locus_tag="ACGI59_000976"
     CDS             292..1818
                     /locus_tag="ACGI59_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFG9738780.1"
                     /translation="MNESYWFLNSSPKEYNKLGENIKTDCLIVGGGITGLTTAYLLAK
                     EGKKVVLVEADKIGYGTSGRNTGKVTCQHDIFYSKIEKKYGLDKAKSYYNANNEALNL
                     VEQIIEENNIKCDFKRETSFVFTEKEDTIKDIKDEYRTCKKIGINCEYHETIENIPLD
                     IKGAISFTNQGQFNPKKYIDGLAKAAVNLGLKIYENTPVVDLEKGKICRVKTREDNII
                     EAENVIISSHSPWYDGLNFYFAKEYAERAYLMVAVLENKLADGMFISIDDPSITFRQY
                     NDGSENLLIFGGGDHKVGQGGTEKEIFDDLEHYGKEVFKVKDFKGKWSAQDNMSFDNV
                     PYIGYINKREDNIYVATGFSKWGITNGTAAGIIIKDLIINNNSDYKDTFNPSRLGSYF
                     SKDFIKENANVAINYVSGKLKIGSGDMPKNNGEGKIVNIDGKRYGVYKDDNGDFYIVD
                     TTCTHLGCELNFNSEEKTWDCPCHGSRFDYKGNILEGPALKPLKLYGHGDNDVNPKLL
                     "
     gene            1846..2403
                     /locus_tag="ACGI59_000977"
     CDS             1846..2403
                     /locus_tag="ACGI59_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9738781.1"
                     /translation="MNKLLKPITILLYIVYILSAVFFVANKEYGSLGLVAISLVGTFI
                     LSFINKQTERLLSKELYIMLALFIMFSSLLGTCFNFYDINYYDDFLHLWSGIISCIVA
                     FSIIKYFYTQREISKMSKVFLVIFLFMFSMGVASLWEIGEFLMDTFVGTTTQAGGLED
                     TMIDMVDALIGTIITIPFIVKKSNI"
     gene            2925..3962
                     /locus_tag="ACGI59_000978"
     CDS             2925..3962
                     /locus_tag="ACGI59_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9738782.1"
                     /translation="MLKKWFGIVKKRQKSESVKEEGEVILKNEKILSEEKLIDEEGVV
                     VNIDNQILTKVVNDDNEIEEKIIEEDLLISENTIKLDDKEAIINDKNIELCKKEVQVE
                     SEKIGLNKFEGLDQNENYNLEKNVIEEKKVSECLTEEDLEYIKEIKIKRGKSIKAINL
                     YTKEEWVFDTHIQCSKKLKVPLGYIRENLKYGYMDYFGDAINYLSEVLNIDEYCKSEW
                     SYLDNSKSPSEIFNILNNKIFSIRLSNEKRNEILTNDKIEALKMNYRFECIDEEYDEY
                     FKKYKSIIKRGGKKKVELVNKKGDILEIFKSLEECAIYLQKEKNEVIQMLKYGDTKVG
                     RNFIRYSLRSI"
     gene            4073..5215
                     /locus_tag="ACGI59_000979"
     CDS             4073..5215
                     /locus_tag="ACGI59_000979"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFG9738783.1"
                     /translation="MSEEKNPKENTLDESKNSDNVNSKKDDVSDEKNTDNKVKKREIK
                     LNNKTLKAIVAIVAVGAICLGGGYVFGKEAGRKLPATSKHYNSSEVVATIGDKKITGE
                     MLRKRMEPLFYLNAKTTMTDDEIRSYESNMIETMITSDILSSEAAKEKISITNDQLDA
                     QYNNLMSSIESAMGMTKEEFLKQFDLTEKYIKDDMKKELVASKYLNEKSKVTDKEAEN
                     YYNKNKSNYLQVRASHILIKTVDDKGKQVSSSKKAELKKEAEEILKKAQAGEDFATLA
                     KKYSEDSSAESGGDLGFFGKGQMVESFEKAAFALKKGEVSNKLVESDYGYHIIKKTDE
                     KYQPFEEVKSDLVSSLTSEKQNLLIQNLKEKYNVKITDKYQMKAKE"
     gene            5669..7060
                     /locus_tag="ACGI59_000980"
     CDS             5669..7060
                     /locus_tag="ACGI59_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HFG9738784.1"
                     /translation="MSSTESVSKKSGHPKSFWLICMTILWERFSYYGLTALLVLFFTA
                     SVAQGGLGLSTQEATSMFGLFTGLIYLTPLVGGWLADKVLGQQKCISIGALLIAIGDF
                     VLFASSTSIAMVWVGLSIIILGNGFFKSSCSNIVGDIYPKTDVSRKDAAYSLFYMAVN
                     VGAFIAPIICGLLSDNVFAVRGASGDIVSYGYKMAFLICGIGMMLGMIIFTLLAPKLL
                     NEVGKYPVTKGNKGEKIEYGPLTKLEKNRIIAMVILFLFVVLFWTAYYQTQSSFMIYV
                     RDNVNRTLFGFEMPVAWLTSLNAILCVALCPVLATLWVKLSHTKRGDFSIPVKMGLGM
                     IIMGVGFIVMVFATLANGPDGMVKASILFIVLTYIFTTVGELFLSPIGLAMFNKLAPA
                     KYATLSMGAWYLTSFFASLLSGKIAGFTATLGFLQIFGGIATVLIIFGLILLGIRNVL
                     TRLMAMDLLTKEK"
     gene            7313..9106
                     /locus_tag="ACGI59_000981"
     CDS             7313..9106
                     /locus_tag="ACGI59_000981"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HFG9738785.1"
                     /translation="MNIKDRLSGLRKFMEEKNIDAYMIPSSDNHQSEYVGDYFKSREF
                     ISGFNGSAGTVIVTKDEAGLWTDGRYFIQAESQLEGSTIKLFKMGQEGCPTTDEYLYK
                     NIPEGGTLGFDGRVISAREGATLAEKLSKKGIKIEYQYDLIDSIWPDRPALSDSKAFL
                     LDVKYCGESFSSKLARLREKMSEKGTSTHVITTLDDIAWLFNIRGGDVKYNPVVLSYA
                     VITLKEVYLFVDESKLNEEILNELAKENVQIKPYNDVYEFVKNIDKTEKVLLDGTKLS
                     YTIYNNIPCEVEKVDEFNPVMFFKAQKNEVELENIRNSHVKDGVAFTKFMYWLKKNVG
                     KMEITEISATQKLEDLRREQEGFFEPSFNTIAAYKEHAAMMHYSATPESNYKLEAEGL
                     FLVDSGGQYYDGTTDITRTTVLGPISDELKLHFTSVARGMINLSKAKFLHGCRGYNLD
                     ILSRSCMWNMGIDYQCGTGHGIGFVLNVHEAPNGFRWRVVPERFDSAVLEEGMVTTNE
                     PGIYIEGSHGIRTENEIVVRKAEKNFYGQFMEFEVVTLAPIDLDGIAPELMNKDEKDY
                     LNWYHKLVYDKISPFLTDEEREWLKVYTRAI"
     gene            complement(9187..10569)
                     /locus_tag="ACGI59_000982"
     CDS             complement(9187..10569)
                     /locus_tag="ACGI59_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HFG9738786.1"
                     /translation="MSVLKTQEKLKQPRDLYFCCSIFTFVGLAYYGMKLVLLLFLAEQ
                     VSKGGLGLTPAESASMLASFLAWTYFSPVIGGWVSDRFLGPKNCIILGMLMVSIGYFL
                     GYIANSKMDINIMIFLSGLGIGFYKGNLHTMVGNLYTNDDPRKDGAFSITYMATNLGT
                     LFGPLICGLVANEWFAMKSGLSISMYGYRYVFLFSSITVLIGLFFFIFGNRKFYKPLN
                     NDPINGRESIRKSLEYKKMLASRPLTLIEKKRIIVILVLAFFTVFFWIAYNQASMSIA
                     LYTKEHINLTVGNFQIPTSWIDSYNGLLCVILGPISALVWVKLSQTKKGDLSVPKKMS
                     LGFILLAIAFLFMIVAVIQTGTDPNSIHKASVFWVVGFLTFQSIGEICFSPVGYGMVN
                     KLSPEKYISFLMGVWFLGKFAANKLSGYTQAIIDQLGMLQVFIVIPSFLLIFGIILLI
                     MNKKLVELSN"
     gene            10827..11174
                     /locus_tag="ACGI59_000983"
     CDS             10827..11174
                     /locus_tag="ACGI59_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFG9738787.1"
                     /translation="MIRKKSELQVEVKENLNGGIGKVKLENIIQNEELKGKGRLFKRV
                     ALPVGSSIGVHDHTTDFEVYYILKGKGKVFDNGEFVEVNEGDVVYTADGEKHSIENIG
                     EEDLEFVALVLYM"
     gene            11192..12226
                     /locus_tag="ACGI59_000984"
     CDS             11192..12226
                     /locus_tag="ACGI59_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6398 domain-containing protein"
                     /protein_id="HFG9738788.1"
                     /translation="MTGKIEEIKDKINRFCNSNLNQEYKDISFKILQNLLDNNKEIIH
                     SSRADIWSSAILNIVLEQNLLYNKKHPLHITKKEFSKQIGVSLNTINNKSSLIKDVCS
                     IDFLNQDTIAISNVEFWVKETNSKSKSVDLDLEKKKILYKRYIKLSQDANSDIESIRY
                     MEEAVNIGKSMITKEDKELGFWKGLSTRAYMIALESLARKLESINNLKESKKVLEYLI
                     EINPSDEQGIRYKLLNVLIRLNDRTSINKLFELYKEEKSATWMYSKALYYFKNKSMFL
                     ANDSIKVARSKNKYVGLYLLDWRNAFGREFVTEEEKAEAVYYYDENIVIWNEVKGSMD
                     WLLKKMLEFS"
     gene            complement(12275..13324)
                     /locus_tag="ACGI59_000985"
     CDS             complement(12275..13324)
                     /locus_tag="ACGI59_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFG9738789.1"
                     /translation="MEINYKKRNFKSFAKRKNIEISVQRYLIDALSYMALGLFSSLLI
                     GTIFNTLGDNFNISLFTEVISPLAKQVTGPAIAVAIAYGLQAPPLVLFSCALVGACGN
                     ELGGPVGAFVATLFAVEVGKLVSKETKIDILVTPAVTILIGVLVAELIGPAVSAFMTS
                     FGNIIMQATTMQPIIMGALVSALVGIALTLPISSAAICMMLSLGGLAGGAATVGCCCQ
                     MVGFAVMSFRENGVGGLVAQGLGTSMLQVPNIIKNWKIWIPPTVASILLGPLSTTIFK
                     MENIPIGSGMGTCGLVGQFGTVTAMQSAGKGGISMWVGILLLNFILPAIVTLLISEFM
                     RKKGLIKPEDLKLDV"
     gene            13599..15134
                     /locus_tag="ACGI59_000986"
     CDS             13599..15134
                     /locus_tag="ACGI59_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9738790.1"
                     /translation="MIFVKNKKYRKIAIIISIPILLVAQYYLFKFGILRPMVKGVEIE
                     IVDGEYVKDIDKYVVKLNETVTLSTGEYIKVPSYAKNPNIWFNVLDNSGTLKIDGNKM
                     TALKVGYSSVGIMKNSRVLKKATIKVVDPEVESLDMEINGDLKHVGDSATIESTIEVD
                     YDKFKKSYKPVYKSSNENILKIKGNKVSAVGVGKATIYAKCGNKEIERTFRIQARVAE
                     INMDDIKIEVDQNEKLKPDIITSPPGLEPPKIYYELVDSKLPVERAISLSSNGNVVGL
                     REGKDRVRITCGDKSKVITVTVVKETITNDYIQNLVGSHKIVNNKVIIKLEWDYMKDV
                     FDYDIYLKDKLSGESFKKIKSITINESDLGKSNKVYATIEVDMKGNQNIDFDIYVIGK
                     KDSDTTKPSNVINITNGNGNSNNDNNIKDMRVENISANVDRDNNVIRVNWSSISHKDC
                     TYSIYVKNNTNGDGEFTLYQSDIQGNEHTISIPEGDLNLDIYVVANYNGNSSQNSDVI
                     NVR"
     gene            complement(15319..16005)
                     /locus_tag="ACGI59_000987"
     CDS             complement(15319..16005)
                     /locus_tag="ACGI59_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HFG9738791.1"
                     /translation="MKTIGLIGGMSWESTITYYQVINTVIKERLGGLHSSKCILYSVD
                     FQEIEECQSSGNWEKSAKILADAAIKLQEAGADFIVICTNTMHKVSDKIQESIHIPLL
                     HIADVTATVLREKEIKKVALLGTKYTMEQDFYKNVIINNGIEVLIPNEEDRIIVNDTI
                     FNELCLGIISESSKKAFLSIIDKLGKQGAEAVILGCTEIGLLIKQNDTSIPLFDTTVI
                     HAIEAALSSI"
     gene            16130..17512
                     /locus_tag="ACGI59_000988"
     CDS             16130..17512
                     /locus_tag="ACGI59_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFG9738792.1"
                     /translation="MPINSFENYPMTWKPKLDNRKPPIYKTLAMLLEEDIKRGNLNPG
                     DKLPPQRELADFLDLNLSTITRAFKLCEEKGLICAKVGKGTFISSDVNVSNTLLYQTE
                     SKDIIELGTVHPPYEQNTYIIDFIKNVLKQPEMDRFLQYMSPSGTYMQKKSIAKWIER
                     NNVYTSEENILLSTGGQNAICATLLGLFKAGDRIATDSLSFSGIKSIAKMIGIQLVPI
                     PQENNEISIEYLESYCKNENIKGIYLIPDYHNPTTHTMSDLSRKKIAKIAKQYNIIII
                     EDAINSIFRDGIQTPIFSLASDNTIYIFSTSKFLCAGLRVAFVVAPKRYIENLENALY
                     NMNLMVSPFTAEIVHRLLYSPIIDKIIEEKRDAIIERNEVADKVLSDYNLIGDKNCSF
                     RWLLLPDELDGKSFEISAKNLGVQVYCAERFSVGNSTVPKAVRICVTAPKDVEELEKG
                     LNIIKSLLKS"
     gene            17914..19182
                     /gene="eam"
                     /locus_tag="ACGI59_000989"
     CDS             17914..19182
                     /gene="eam"
                     /locus_tag="ACGI59_000989"
                     /EC_number="5.4.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016869 - intramolecular aminotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006536 - glutamate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 2,3-aminomutase"
                     /protein_id="HFG9738793.1"
                     /translation="MNEQTRISLERAAELKSKIDDYIQARKTINRGLEKEEEINKRKQ
                     KILSILNGTEEDWNNYKWQLSNRITDVDTLSKIITLTKKEKEYIKEVGTQFRWAISPY
                     YLSLIDPEDTCDPIKLLSIPTHIELEDEQEDLDPMGEEYTNPAGCITRRYPDRLIINV
                     TNECAMYCRHCQRRRNIGQQDSHKSKAIIQESIDYIRENEEIRDVLVTGGDALTLKDD
                     YLEWILSQLKEIPHVDYVRLGTRTLVTMPQRITDEFCNMLKKYHPVYINTHFNHPMEI
                     TKESKEACEKLANAGVPLGNQAVLLNGINNDKFVMRCLNQELLKIRVKPYYIFQSKHV
                     KGTKHFNTSVDDGLEIMEYLRGYTSGMAIPTYIVNAPKGGGKTPLLPQYLVSKGTDYV
                     MLRTWEGKVIKMEDEPAVDIKKLIKEQAQD"
     gene            complement(19275..19631)
                     /locus_tag="ACGI59_000990"
     CDS             complement(19275..19631)
                     /locus_tag="ACGI59_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9738794.1"
                     /translation="MKKALISLLVLSCLLLTAPVNESSYADSQKNVPVSQNTKLTQEQ
                     AKELLVKYNNEVDYIYQGNASDFEALKAKNLNGYVFLPDADGDIGYFVNENTSEIYYF
                     HPSGYLELVNQQQNNK"
     gene            complement(19782..20876)
                     /locus_tag="ACGI59_000991"
     CDS             complement(19782..20876)
                     /locus_tag="ACGI59_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFG9738795.1"
                     /translation="MQDKKIYLNIVLTVVVSYILIKVIDNYKYFFGVISLLMSLLTPF
                     IIAFVLAYIFNPLVKFLESKLNFKRIYSLLLTYGVLIVILISFILFTVPSIVNSLADL
                     VAQIPTYIDKTEQFLFDLGKSLQSVDPNTLKEYGDKIMSVMPKFSNLLIGSLGGIFST
                     TFSVGKFIVQFLLGFIICFYILLEKEKFLLFTKKVVYISLGKKYGDFIIELCQSLNLN
                     IGKYFTGKILDSFIVGVLSGIGLYFLKSEYALLFGTLMGVMNMVPYFGPVIGMAPVVI
                     INLFSNPTIALTSLIYLIIIQQVEVTFIEPKIVGGQLGLSPFFTILAVTVGGGFFGIP
                     GMILSAPVMGVIKIYFCEFVNRRHDKIQME"
     gene            21340..22161
                     /locus_tag="ACGI59_000992"
     CDS             21340..22161
                     /locus_tag="ACGI59_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFG9738796.1"
                     /translation="MIKKEISNFRGSSDYVVSPELMASVNVAIALEKPLLIKGEPGTG
                     KTMLAQAISNELKKDLVIWNIKSTTKAQEGLYVYDTVQRLYDSQFGGEGVDDISKYIK
                     YGKLGEAFSSNQQVILLIDEIDKADLEFPNDLLWELDKMEFYINETKETVRAKQRPIV
                     IITSNAEKELPDAFLRRCIFHYIEFPDRDMMEEIVKVHFDKVEEHLLEQVMTTFYWIR
                     SLKDIQKKPSTSELIDWIQALTLSGMPIEKIEKEVPFAGILLKNNEDIESMQRHL"
     gene            22182..23378
                     /locus_tag="ACGI59_000993"
     CDS             22182..23378
                     /locus_tag="ACGI59_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFG9738797.1"
                     /translation="MFIEFFYLLRSRGLNVSLNEWMTLIEALDKGLCYSNFSNFYYLC
                     RMILIKSESDFDKFDAVFLEYFKGIEHQEEIPEEIMNWLKKPDIDLEEFERLEQNPNI
                     NLDKLRAKLEERIKEQDSPHNGGNYWIGTGGTSELGHSGKGQTGIRIGGNSTYGRAVL
                     EVAGERKYRDFRDDEVLNMRQFQVALKRLRQFSTRIDAPKTELDLDKTIEETCNNAGY
                     LKLFFEKPRKNTVKLLLLMDSGGSMRGYSTLCNTLFQSVSKSNHFKDVKIYYFHNCIY
                     DRLFTTPECWLSKSINTEWILKNIDKNYKVIIVGDASMSPSELLHIGGNYRGPYNYTP
                     GIEWLKRFKRKYSKIVWMNPELRDGWDSISYWYQTQRMIQSEFNMFPLTVKGLEKALK
                     NLMVSR"
     gene            23545..26931
                     /gene="cspBA"
                     /locus_tag="ACGI59_000994"
     CDS             23545..26931
                     /gene="cspBA"
                     /locus_tag="ACGI59_000994"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional germination protease/germinant
                     receptor pseudoprotease CspBA"
                     /protein_id="HFG9738798.1"
                     /translation="MIIINYELIVKYNGDILRLEEELGVSVEILNSSYAIITSSNEED
                     VNILLTYPEIEFIEKPFILQTQDVQSFSSTGITGFKNRTGLTGKGTIIGIIDSGIDYT
                     LPVFRDSDGRSKILYYWDQSIQGNPPEGFREGTLYTNEDINNAIDGSMYIPISTTSLH
                     GTHVAGICATIASDARIIVVRVGNIQTDIFSRSTEFMRAIKFILDRALELRMPVTLNI
                     SYGSNEGSHRGTSLFEQYIDDMCLFWKNNIVVAAGNNADKGGHKRIRLQNNITEEVEF
                     IVGEGERILNINIWPDFVDDFSVHLVNPSNNQTQAISLTSGEIRNTLGETRITGYFYP
                     IAPYSLTRRVTLQLSSNTQITPGLWKIVFEPIDIVTGNVNIYLPTSEGLNRNTRFLIP
                     TQELTVTVPGTASRVITVGSFNSRTDIVSIFSGEGDTQLGVFKPDLLAPGEDIVSFLP
                     GGTSGALTGTSMATPHVTGVCSLFMEWGIVNGNDLFLYSQKLRALLLKGARRLSNQSY
                     PNNSSGFGFLNLSDIDLYTLSNINQDLETEDIGYRSINKSFKDEENSYKFIDGYNMQI
                     HNDLENEIYISKNASRQSGIDIVHTPEFEEELAGLGMSQRFFKISDSLGVLSINNTDY
                     NSIQRVLQLPSIIRTVSTTKMTLLGEINRGTFGGVVATEEMGVNFFKNNPNINITGRG
                     TLISIADTGIDYLHPDFIYPDGTSKIVYLWDQTKEGTPPDGFYIGTEYTREDINRAIA
                     ENDPSLSQDEVGQGTMLSGICAGLGNVNSEYAGIAEDSELIIIKLGKIDDFYNSAMLF
                     AASQYAYKKAFELGRPLVINMSLGTSSLAGLTNRSNSEKAFFTRGLCITAGAGNEGNT
                     QTHTSGIIPYVGGSVEVELELNEDEEELSLELWLNRPDKADVIIVSPTGEESKSVGIS
                     NYNKVTGLFDLEGTEYSITYIYPTTFSGQQFTNVTLKNAKRGVWKIRLVGVYIITGRY
                     NLYLPNRELLKSGTRFREVDPFYTINYPAIQDDLITIGAYNTINGSLWQSSSRGPTIE
                     DRLKPDIVAPGVNIIAAYPGNTYATITGTAAASAHAAGAAAMYFQYTFVDGRYPNQAY
                     VQKIKTFMQAGARKDSNTVYPNTNSGYGLLDVRGMFDVLR"
     gene            26941..28614
                     /gene="cspC"
                     /locus_tag="ACGI59_000995"
     CDS             26941..28614
                     /gene="cspC"
                     /locus_tag="ACGI59_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid germinant receptor pseudoprotease
                     CspC"
                     /protein_id="HFG9738799.1"
                     /translation="MEKSYCIIYQGDIESALQENGINRYMVLNSQLAVIYVPLDFDET
                     ILNNIIQVAWWEESEPMSSLIEITNNVNNGETITTAAETDYIYENPYNDITGRGILLA
                     VIDSGIDYLHPDFINDDGTSKVLYLWDQEANTNPPPEGFIFGSEFTRSELNIAINRND
                     GSLSQDNIGTGTLVSGILVGNGRINSQYRGITTESDLIVVKLKSYTDTYYAGRINYSV
                     SDFLAAITYVTNIARTENKPLIINLTIGVKSSAVATTSILDTFNILSSAGVVVVSGAG
                     NQGNTDIHYSGRFSSVGEVQDVIIQDGDDYALDITLNTNGPDKVGAQIISPSGEVSHD
                     IRYSPDFYIYRGKFNLENTTYAMRFIYPYITSGKENLEIRLRDIKPGVWILRLTSELI
                     INGEYDIYLPNKNLIAPDTRFLEPDSVATITMYAASDDVITVGTFNNKTDSMWIGSSK
                     GPIRGRGIKPDIVASGVDIISTYKNGTYNTGTGTGVSSSIVTGVLALLMEYLEKQDNV
                     PRLSLFTQVLKTYLILGATKLEIYTYPNVSQGYGILNLKNTIQQIANTL"
     gene            28887..29081
                     /locus_tag="ACGI59_000996"
     CDS             28887..29081
                     /locus_tag="ACGI59_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3787 domain-containing protein"
                     /protein_id="HFG9738800.1"
                     /translation="MESKKARALKGKDNKRLKSSLLVGNESTAAWADVEKLKPHSKVS
                     IPSIKGVEEAKDWVDNGSKL"
     misc_binding    29250..29483
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            29560..30957
                     /gene="asnS"
                     /locus_tag="ACGI59_000997"
     CDS             29560..30957
                     /gene="asnS"
                     /locus_tag="ACGI59_000997"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFG9738801.1"
                     /translation="MQKEFITVKELYREKEQYIGKTVKVAGWIRTSRVSKNFGFIELN
                     DGSFFKNMQIVFDEKVENFKEVGKLAISSSILVEGELVSTEGAKQPVEIHAKSIVVEG
                     ESDSSYPLQKKRHTLEYLRTIAHLRPRSNTFSAVFRVRSIAAYAIHKFFQERNFVYAH
                     SPIITGSDCEGAGEMFRITTMDLNDIPKTEDGKIDYTQDFFGKEANLTVSGQLNAEIM
                     ALSFRNVYTFGPTFRAENSYTGRHASEFWMIEPEIVFADLEDNMELAEDMIKYVISYV
                     LENAPEEMEFFNSFIDKGLLERLNKIVNSEFIRITYTKAVELLIESGHEFEYPVEWGC
                     DLQTEHERYITEQIYNCPVFVTDYPKDIKAFYMRMNEDGKTVRAMDLLVPGVGEIVGG
                     SQREEREDVLLDRINEMGLREEDYWWYLELRKFGTATHSGFGLGFERIIMYMTGMSNI
                     RDVIPFPRTPKNAEF"
     gene            complement(31130..32305)
                     /locus_tag="ACGI59_000998"
     CDS             complement(31130..32305)
                     /locus_tag="ACGI59_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFG9738802.1"
                     /translation="MNLEKIKKFFGFPLDKKHKDNVEKELCNYLYHTGKIICIIIVIS
                     QIIMMISISAREGGPFVTPRRQWYFYMYVTLFCVSSVFMFVFSYLWNKKKDCCTFYIN
                     LWIIYVTFFCLWGCVITLLDQLGGNDLSVFSYIMLAAAAFSVLKFGESVIVFGGCFLF
                     LNLFLPGIQTIPGNEFSNIVNTFFITSLAIFVSTMLYRNRVFTYYDKLLISQQYEEIS
                     HINNLLNDKVMTDDLTKMNNRRYLEDILQKKLISHANQEPITGMMIDIDYFKQFNDNY
                     GHQKGDYCLQNIAKVILDFTSSENEHAIRYGGEEFFICIFNCNEADAKLKSECLRKII
                     ESCNFERDDVPLKCVTVSIGVYTKKIGKTITLNEFISNCDKALYTAKEQGRNCVAFY"
     gene            32724..34088
                     /locus_tag="ACGI59_000999"
     CDS             32724..34088
                     /locus_tag="ACGI59_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFG9738803.1"
                     /translation="MGEKKINNMNYSHNNYSKKTGEPTRKIKNSHITLKSKEEKICSS
                     CNKENNTKDNYCKFCGNELYEIASLRPLETKLDLKSKIKELSYHANKRGVFLTTFTTI
                     FILFIIALIFKAIITIQFNDISYLINPAHIILALNLGQISVSMSTMMGSGFINANIGL
                     LILLIIPILVLLISNLIFMRKRCRDSKTVLANSLGVGIFYGLVLAILSIFTNVKTSSH
                     SMLEYGYALEYSYEFFSVLLNGFVLGFICTYITTYKKSYEKENMYLSLFSNSIRTFIL
                     GYVLVLVILLVLTISDSSYLNELDMSSYSNGLNLFTILPQIASYMWAFANGISVTIIN
                     STVSMFTLSSSSLFGDTKLMFYAMGALSMLILLLNGYKLRFKYNTDSIRPIIVFSIYY
                     AFLMGILALFSTFILDSNINFFNTTNYGTTLIMQFKVLQAIVISFVYSFVISLIGYKL
                     NSAD"
     gene            34110..34601
                     /locus_tag="ACGI59_001000"
     CDS             34110..34601
                     /locus_tag="ACGI59_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFG9738804.1"
                     /translation="MEGNTNNKVDDKFSKVKKKFGSGKFKVELYKEKSKLNREIDDIQ
                     EKKAKIFLEMGILTYQKIREDSINDNSFDKFCDELLELDKLIYEKNMEINEMEMAESN
                     VTCECGYVGNVNDKYCAECGKKFELENECDDFIICGYCESEIDTEAEFCPCCGRKIIM
                     DLE"
     gene            34874..35545
                     /locus_tag="ACGI59_001001"
     CDS             34874..35545
                     /locus_tag="ACGI59_001001"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFG9738805.1"
                     /translation="MLDKVKGRLIVSCQALEDEPLHSPFIMGRMAKAAMEGGAVGIRA
                     QGVEDIIEIKKVTGLPVIGIIKRNYEDSDIYITPTKKEVDELLTTGCEMIALDATNRV
                     RPNNEDLKELIKYIKENGVLVMADISNYDEAIKAQEYGVDCVSTTLSGYTPYTKTLEG
                     PDFVLMERLVKDLEIPVIAEGKVNTPQDLKKVFELGVHSSVVGSAITRPQLITEKFVK
                     AIEIN"
     gene            35615..36496
                     /locus_tag="ACGI59_001002"
     CDS             35615..36496
                     /locus_tag="ACGI59_001002"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HFG9738806.1"
                     /translation="MRTTDMKGIYSALLVSFDKEGNINEKGLRQIIRHNIDVCKVDGL
                     YVGGSTGENFMLSTDEKKRIFEIAKDEVKEEIKLIAQVGSVNLKEAVELAKFTTDLGY
                     DAISAVTPFYYKFDFEEIKHYYNTIINSVDNRLIIYSIPFLTGVDMSLDQFGELFENE
                     KIIGVKFTAADFYLLERMRKTFPNKLIFAGFDEMMLPATVLGVDGAIGSTFNVNGVRA
                     RQIFELTKNEKISEALEVQHVTNDLITDILGNGLYQTIKLLLEEQGVEAGYCRQPMKE
                     ATDEMKSRAKEIYRKYF"
     gene            36529..38031
                     /locus_tag="ACGI59_001003"
     CDS             36529..38031
                     /locus_tag="ACGI59_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HFG9738807.1"
                     /translation="MVGFTWIDFAILVVYLLAVLFAGLLFSKKEMKGKEFFKGDGTIP
                     WWVTSVSIFATLLSPISFLSLAGNSYGGTWILWFAQLGMFIAIPLTIKFFLPLYSRLE
                     IDTAYEYLEMRYESKGLRVLGALMFIIYQIGRMSIIMYLPSIVLAGLTGIPVNVLIIV
                     MGVIAIIYSYTGGLKAVLWTDFIQGMVLIVGVTGTLFYLIASINGGFGTVMDTLTTGH
                     KFLASNEVMFDKNILSTSAFIIFVGAGLNTFSSYVSSQDIVQRFTTTTDIKQLNKMTY
                     GNGVLSMGLATVFYLIGTCLFIYYTQNPDLAQTVQQDQVFASYIAYELPVGITGILLA
                     AIYAASQSTLSTGLNSVATSWTLDIQTIITKDISMEKQTKIAQYISLGVGILSIAVAI
                     VLANGEIKSAYEWFNSFMGLVLGVLAGIFVLGAFCKKATKMGAYVGFIVSAILVVYLK
                     YRMPEVTSWAYSLITITTSVVVGTIVSAIEAKVKAKVSLPKAETTVYYEK"
     gene            38161..38613
                     /locus_tag="ACGI59_001004"
     CDS             38161..38613
                     /locus_tag="ACGI59_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HFG9738808.1"
                     /translation="MIFGNINHSKTYSTLHEDLLRCFEYAKDNSLIDYEKGTYTIDGD
                     DIFVNIVEYKTCEKEDRFWEAHKKYIDVHLMLDGCERIDINFIENLEQKIYEEKGDFL
                     PLDGKNNGYMELRKGDFLVCYPEDAHMTGIKVLEKENIKKAIFKVLVR"
     gene            38773..39576
                     /locus_tag="ACGI59_001005"
     CDS             38773..39576
                     /locus_tag="ACGI59_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFG9738809.1"
                     /translation="MENIDVFLLIGQSNARGLGNPKESIIPNENCFEYLSTDEIINMR
                     CELETSEGDGTIAPAFSNEWNKLTGNKVCFIHNAKDGSRIKNWNHDNNWFLNDTIEKF
                     NTGCTTLSKHYNIENKYVIWIQGESDAKYGSDVLYYKESLKKIAYRLKEECMIDKMFV
                     SLTGYWLGEDEYFIRTRRIAAAQESACSECDILCVGSKIAMQFHDRGLTIDDVHYTQE
                     GLNMLGEDLCKNIYKYHITKEKSVLSDTIDLSEARKYICELEKISKRFV"
     gene            39634..40521
                     /locus_tag="ACGI59_001006"
     CDS             39634..40521
                     /locus_tag="ACGI59_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFG9738810.1"
                     /translation="MKNYVCIDVGGTSIKYGFIREDGFIIDKSSLDTEAKEKGGEGIL
                     AKIKDIVKKYIEENEISGICISTAGMVDPVEGKILFALEELIPNYKGMQLKKEVEKEF
                     NIKCEVENDVNCAGLGEMWLGAGKDATSSICLTIGTGIGGCIIINNKLINGFSNSAGE
                     VGYMNVNGSSFQELASTSSLIKKVAKIKNLNENDLNGKIIFDLAKNNDEDCLKELDNM
                     IKSLAVGIANICYVINPEVVILGGGIMAQEKFLKPKIDKALKEVLIERVYKNTNIEFA
                     KRQNDAGMLGALYNFLNKI"
     gene            40540..41388
                     /locus_tag="ACGI59_001007"
     CDS             40540..41388
                     /locus_tag="ACGI59_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFG9738811.1"
                     /translation="MSILEQLESPTFKVTKSDKLLIAYIKENIDDVFYKPISQIAKES
                     NIGEATITRFVKKMNFNGLQDFKVTLAQEISTINKKNIINKNIQNDEPALDTAKKLLS
                     SNVTTLENTVEIINSKDVHDCARLIINAKKVYFIGIGYSGIIAQDSNYKFMRIGLNCV
                     SFDSSHTMIMMSSIMEEGDLIIAISHSGETEEIIKTVKLARANNAKIISITENKNSEL
                     KDISDVHLSYVSGETVLETGSISSKLAQFFIIDLVYTQVVKELSNEAIERKIKTTNAI
                     KLFKNE"
     gene            41451..42140
                     /locus_tag="ACGI59_001008"
     CDS             41451..42140
                     /locus_tag="ACGI59_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFG9738812.1"
                     /translation="MSKLKDYNIDKVIIFNNLSTKTKNQLKEKVKLKRLQKKEILFYE
                     KDSLDSVYILLEGKVSIFKISENGERKVIFILNSGEVINDITLDASKNSTVGCEAFEN
                     SIVAYYSIEEFLEIMQNDFQLTKDIISYMERRIRRLYRQLKNSISIKVDKKLAAKLYR
                     LSREFGVCCGEWTLLNVNITVTYLADMLGCKRETVSRMIKVLQKEELIKIDNKGFYVK
                     ETELSRYFKNN"
BASE COUNT        16649 a         4603 c         7231 g        13680 t
ORIGIN      
        1 actgattgca taaaaatgat ttttatataa aatttaagca gaagaataaa tttctaacga
       61 aattggatat ttcttctgta aatacatatt caaaaaaaga attataagag tttagtcagc
      121 agtcgtgtaa gtaataagga ctttataaat atatagaaat ataatgatat taatataacc
      181 aagcgatttt taatttagct ttttaataag cttatttttt atactattta taataaataa
      241 actccatgat gggaaaacta ttactattta atatgtaaag gagtatttta aatgaatgaa
      301 tcatattggt ttttaaactc aagtccaaaa gaatataaca aattaggtga aaatataaaa
      361 acagattgtc ttatagtagg tggtggaata acgggattga caactgcata tttattagca
      421 aaagaaggta aaaaagtagt gcttgttgag gctgataaaa taggatatgg aacttctgga
      481 aggaatactg gtaaggtaac ttgtcagcat gatatatttt actctaagat agagaaaaaa
      541 tatggattag ataaagcaaa atcatattat aatgcaaata atgaagcttt aaatttagtt
      601 gaacaaataa tagaagaaaa caatataaaa tgtgatttta aaagggaaac ttcttttgta
      661 tttacagaaa aagaggatac tataaaggat attaaagatg aatatcgtac atgtaaaaaa
      721 ataggaataa attgtgagta tcatgaaact atagaaaata ttccattaga tataaagggg
      781 gctattagtt ttactaatca aggtcaattt aacccaaaga agtatattga tggattggca
      841 aaagcagcag taaatttagg gctaaaaata tatgaaaata caccagtagt agaccttgaa
      901 aaaggaaaaa tatgtagggt taaaacaaga gaggataata taatagaagc tgagaatgtt
      961 ataatttcat ctcatagtcc atggtatgat ggattgaatt tttattttgc aaaagaatat
     1021 gcagagcgtg cttatttaat ggttgcagtt ttggaaaata aattagcaga tggtatgttt
     1081 ataagtatag atgacccatc aataactttt agacaatata atgatggaag tgaaaattta
     1141 ctgatttttg gaggaggaga ccataaagtt ggtcagggag gaactgagaa agaaatattt
     1201 gatgatttag agcattatgg taaagaagtc tttaaggtaa aagattttaa ggggaaatgg
     1261 tctgctcaag ataatatgag ttttgacaat gttccttata ttggttatat caacaaaaga
     1321 gaagacaata tttatgtagc tacagggttc tctaaatggg gaattactaa tggaactgct
     1381 gctggaataa taataaaaga tttaataata aataataatt ctgattataa agatactttt
     1441 aatccatcca gactaggtag ctacttttca aaagatttta taaaggaaaa tgcaaacgta
     1501 gcaataaatt atgtaagtgg aaaattaaaa ataggtagtg gagatatgcc taaaaataat
     1561 ggagaaggaa agattgttaa tattgatgga aaaagatatg gtgtatataa ggatgataat
     1621 ggagattttt atattgtaga cactacatgt actcatttag gatgtgaact taattttaac
     1681 agtgaagaaa aaacatggga ctgtccatgt catggttcta gatttgacta taagggaaat
     1741 atacttgaag gaccagctct taagccatta aaactttatg gacatggtga taatgatgtt
     1801 aatcctaagt tattataaaa ttatttttag gaaggtaaag tgtatatgaa taaactttta
     1861 aagccaataa ctatactatt gtatatagtt tatattttat ctgccgtatt ttttgtagca
     1921 aacaaagaat atggaagtct aggtctagta gcaattagtt tagttggtac tttcattttg
     1981 agttttataa ataagcaaac tgaaagatta ttgagtaaag aattatatat aatgcttgct
     2041 ttatttataa tgttttcatc attgttaggt acttgtttta atttttatga tataaattat
     2101 tatgatgatt ttttgcattt atggtcaggt ataatatctt gtattgtggc tttttctata
     2161 attaaatact tttatacaca aagagagata agtaaaatga gtaaagtgtt tttagttata
     2221 tttttattta tgtttagtat gggagtagcg agtttatggg aaataggtga atttttaatg
     2281 gatacttttg ttgggactac tacacaggcc ggagggttag aagatactat gattgatatg
     2341 gtagatgctt taataggcac aattataact attcctttta tagtaaaaaa atctaatata
     2401 tagttttaaa ttttttatat taattataat ggatttaatt aatcatgttg agtttcaagg
     2461 tctatatgat taattaaatc catttttgtt atttataatt attattgaat atatttttac
     2521 atataagata taaggtgata gatagaaatt gttggattat gtaaataatt aatatataat
     2581 accattatgg gcaataaata caaatcaaat gataatatag tctatttatg taaatgttat
     2641 attgtatcct aagtataata gtaagttttt tgatagtggt gtagaaagta aaaatctact
     2701 agcctaaatt gatgtatgga ttagtttagg aacagtaatt gcatatctta agtttgatgt
     2761 catgttgggc tactagaaat aggtcatctt tttcacttga atatcttttc tttaaacatg
     2821 gggagtatca tatcaaaaac atatgaaaat taagtgattt atgagaacag taatatttta
     2881 tatgatagta tagaatagta ataaagtatt aggaggaaca ttgagtgtta aaaaagtggt
     2941 ttggtattgt gaaaaaaaga caaaaaagtg agtcagtaaa agaagaaggt gaagtaatat
     3001 taaaaaacga aaaaatatta tctgaagaaa agttgataga tgaagaagga gttgtagtta
     3061 atattgataa tcaaatatta actaaagtag taaatgatga caatgaaata gaagaaaaaa
     3121 taatagaaga agatttgtta attagtgaaa ataccataaa attagatgat aaagaagcaa
     3181 ttattaatga taaaaacata gaattatgta aaaaagaagt tcaagttgaa agtgaaaaaa
     3241 taggtttaaa taagtttgaa ggactggacc aaaatgaaaa ttataatcta gaaaaaaatg
     3301 ttattgaaga aaaaaaagta agtgaatgtt tgacagaaga agatttagag tacataaaag
     3361 aaattaaaat aaaaagagga aaaagtataa aagctataaa cttgtatact aaagaagagt
     3421 gggtttttga cactcatata cagtgtagta aaaaactcaa agttccatta gggtacataa
     3481 gagaaaattt aaaatatgga tatatggatt actttggaga tgcaataaat tatttaagtg
     3541 aagtattaaa tatagatgaa tactgcaaaa gtgaatggag ctatctcgat aatagcaaat
     3601 ctccatctga gatatttaat attctaaaca ataaaatatt tagtataagg ctttctaatg
     3661 aaaaaagaaa tgaaattttg acaaatgata aaattgaagc attaaaaatg aattatagat
     3721 ttgaatgtat tgatgaagaa tatgatgaat attttaaaaa atataagtct ataatcaaaa
     3781 gaggtggaaa gaaaaaagtt gaattagtaa ataaaaaagg tgacatttta gaaatattta
     3841 agtctttaga agaatgtgcc atttatttgc aaaaggaaaa gaatgaagtt atacaaatgt
     3901 taaaatatgg agatacaaaa gtaggaagaa actttataag gtatagtttg agaagtattt
     3961 aatttcattt taaattcata agagtttcat ttctgattca cacatttaat attattttaa
     4021 tgtaaaataa taaaggtgaa gtattgcaca attaaaatta aggagggtat taatgtctga
     4081 agaaaaaaat ccaaaagaaa atacattaga tgaatctaaa aattcagata atgtaaattc
     4141 taaaaaagat gatgtgtcag atgaaaaaaa tactgataac aaagttaaaa aaagggaaat
     4201 aaagctaaac aacaaaacac ttaaagctat tgtagcaatt gttgcagtag gagctatatg
     4261 tttaggtgga ggctatgttt ttggcaaaga agctggaaga aaattgcctg ctacttcaaa
     4321 acactataat agcagtgagg tagtagctac tattggggat aaaaaaataa caggtgaaat
     4381 gttaagaaag agaatggagc cattatttta tttaaatgca aaaactacta tgactgatga
     4441 cgaaatacgt tcatatgaat caaatatgat tgaaactatg attacatctg atatattatc
     4501 ctctgaagca gcaaaagaga aaataagtat aactaatgac caactagatg ctcagtataa
     4561 taatttaatg agtagtatag aatcagcaat gggaatgact aaagaagagt ttttaaaaca
     4621 gtttgatttg actgaaaaat atataaaaga tgatatgaaa aaagagctag tagcatcaaa
     4681 atatttaaat gaaaaatcaa aagttactga taaagaagca gagaactatt acaataaaaa
     4741 caagagcaat tatttacaag ttagagcttc acatatttta ataaaaactg ttgatgataa
     4801 aggtaagcaa gttagtagta gcaaaaaagc tgaacttaag aaagaagctg aagaaatact
     4861 taaaaaagcc caagctgggg aagattttgc tactctagct aaaaaatact ctgaagatag
     4921 tagtgcagaa tcaggtggag atttaggttt ctttggtaaa ggtcagatgg tggaatcttt
     4981 tgaaaaagca gcctttgcat tgaagaaggg agaggtatct aacaaacttg tagaaagtga
     5041 ttatggatat catataatta agaaaactga tgaaaaatat cagccatttg aagaggtaaa
     5101 atcagattta gtaagcagtt taacatcaga aaaacaaaac ctacttatac aaaatctaaa
     5161 agaaaaatat aatgttaaaa ttacagataa atatcaaatg aaagctaaag aatagataaa
     5221 gtcaatttat taaaaaaaag aaaaattaac tgtatggata aaagctattt ttactctcaa
     5281 tatagagagt ataaaatagc ttttattatt ttgcaataaa atctatttat atatatgaag
     5341 tgataaagaa ataacatgca aaaaggtatt aaaaagaaat atttttaatt tgaatttttt
     5401 caataaaaat ttacaaaaat gaaaaaataa acaaaaaaga attgaagatt taagttgtta
     5461 tttaaatata tatatattaa gattttctgc tgaagatact aaaaaaataa tatttttcat
     5521 aattttaaaa ataataataa aaaaatgaaa atacaggcta taatctattg agtaaacata
     5581 gaaaagatga tattataagt atgtgctaaa atggttaaag caatattaat attaaattta
     5641 actataaaac aaaagggggc gtttttttat gtcaagtaca gaaagcgtat ctaaaaaaag
     5701 tggtcatccg aagagttttt ggcttatttg catgacaatt ttgtgggaaa gatttagtta
     5761 ttatggttta acagctttat tggtattatt ttttacagca agtgtagctc aaggtggttt
     5821 aggtctttct actcaagaag caacatctat gtttggctta tttacaggtc ttatatatct
     5881 tacaccatta gtcggtggct ggttagcaga taaagtttta ggtcaacaaa aatgtatttc
     5941 tataggtgca cttttaatag caataggaga tttcgtacta tttgcttcat ctacatctat
     6001 tgcaatggtt tgggtaggtc tatcaataat tattttagga aatggttttt ttaaatcaag
     6061 ttgctcaaat atagttgggg atatatatcc taaaactgat gtgtcaagaa aagatgcagc
     6121 atacagttta ttctatatgg cagtaaatgt aggagcattt atagctccaa taatatgtgg
     6181 attgctttct gataatgtat ttgcagtaag aggagcaagt ggagatatag tatcttatgg
     6241 atataagatg gcatttttaa tttgtggtat aggcatgatg ttaggtatga ttatatttac
     6301 acttcttgca ccaaaattat taaatgaagt cggaaaatat cctgtgacta aaggtaataa
     6361 aggtgaaaaa attgagtatg gaccacttac taagttggag aaaaatagga taatagcaat
     6421 ggttatttta tttttatttg tggtcttatt ctggacagca tactatcaaa cacaaagttc
     6481 ttttatgatt tatgtaaggg ataatgtaaa tagaactcta tttggatttg aaatgccagt
     6541 agcatggctt acatcactaa atgctatatt atgtgtggct ttatgcccag tattagctac
     6601 cctatgggtt aaattatctc ataccaaaag aggtgatttc tctattccag ttaaaatggg
     6661 attaggaatg ataataatgg gtgttgggtt tatagttatg gtatttgcta cattagctaa
     6721 tggtccagat ggaatggtaa aggctagtat attgtttata gtattgactt atatatttac
     6781 aacagtaggt gaattattct tatctcctat aggacttgca atgttcaata aattagctcc
     6841 agctaagtat gctacgttgt ctatgggagc ctggtatcta acaagttttt ttgcaagttt
     6901 attatcagga aagattgctg gttttacagc cacacttggt tttttacaga tatttggagg
     6961 aatagcaaca gttttaataa tatttggttt aatattattg ggaataagaa atgtattaac
     7021 aagattaatg gctatggatt tactaacaaa agaaaaataa ttcatgacga attattgtaa
     7081 ataaatgaat tactttaaat aaaggaagtt acatttaaat ataaaattaa atgtaatttc
     7141 ctctttgatt atgaaaaaaa gctttacaca aatagatatt ataattaaaa ttataattgt
     7201 gataattaga gtgtataaga tttatataaa ttatacaaaa aatatttatg taataaactt
     7261 tacatactta tatatttagt tattcaaact attatttcga ggaggtcttg ttgtgaatat
     7321 aaaagataga ttaagtggat taagaaaatt tatggaagaa aaaaatatag atgcctatat
     7381 gattccatct tctgataatc accaaagtga atatgtagga gattacttca aatcaagaga
     7441 atttatatca gggtttaatg gttcagcagg gacggttatt gtgactaaag acgaagctgg
     7501 gttatggaca gatggaagat attttataca ggcagaatct caattagaag gaagtacaat
     7561 aaaattattc aaaatgggac aagaaggttg cccaactaca gatgaatatt tatataaaaa
     7621 tattcctgaa ggaggaactt taggatttga tggtagagtt atatctgcta gagaaggagc
     7681 tacattagct gaaaaattat ctaaaaaagg tataaagata gaatatcagt atgatttaat
     7741 agatagtata tggccagata gacctgcttt atcagatagt aaagcatttt tattagatgt
     7801 taaatattgt ggagaaagtt ttagctcaaa attagctaga ttaagagaaa aaatgagtga
     7861 aaaaggtact tctactcatg taataactac tcttgatgat atagcatggt tattcaatat
     7921 aagaggtgga gatgttaaat ataatccagt tgtactttct tatgcagtaa taactttaaa
     7981 agaagtttat ctatttgttg atgaaagtaa gttaaatgaa gaaatactaa atgaactagc
     8041 taaggaaaat gttcaaataa agccatataa tgatgtgtat gaatttgtaa aaaatataga
     8101 caaaactgaa aaagttttat tagatggaac taagcttagt tatacaattt acaataatat
     8161 accttgtgaa gttgagaaag ttgatgagtt caatccagta atgtttttta aagctcaaaa
     8221 aaatgaggta gaattagaaa atataagaaa ttcacatgta aaagatggtg tagctttcac
     8281 taaatttatg tactggttaa agaaaaatgt aggtaagatg gaaatcactg aaataagtgc
     8341 tactcaaaaa ttagaggact taagaagaga gcaagaaggt ttctttgagc caagtttcaa
     8401 tacaattgct gcatataaag agcatgctgc aatgatgcac tatagtgcaa ctccagagtc
     8461 aaattataaa ttagaggcag aaggattatt tttagtagat tcaggtggac aatattatga
     8521 tggaactact gatataacta ggactacagt tttaggacct ataagtgatg aattaaaact
     8581 acactttact tctgtagcaa gaggaatgat aaatctttct aaggctaagt tcttgcatgg
     8641 ttgtagagga tataacttag acatactatc tagaagttgc atgtggaata tgggaattga
     8701 ttatcaatgt ggaactggac atggtatagg atttgtgcta aatgttcacg aagctccaaa
     8761 tggatttaga tggagagtag tacctgaaag atttgatagt gcagttcttg aagaaggaat
     8821 ggtaactact aatgaaccag gtatatatat agaaggttct catggtataa gaacagagaa
     8881 tgaaattgta gttagaaaag ctgaaaagaa tttctatggt caatttatgg aatttgaagt
     8941 tgttacgtta gctcctattg acttagatgg tatagcacca gaattaatga ataaggatga
     9001 aaaagattat ttaaattggt atcataagtt agtttatgat aaaatatcac ctttcttaac
     9061 tgatgaagaa agagaatggt taaaagttta cactagagct atatagtttg tactagatac
     9121 aataaaaatc gaggtattat aatgcctcga ttttttattg tatattttat taactttaaa
     9181 aaatttttaa tttgataatt ctactaattt tttattcatt ataagtagta taattccaaa
     9241 tatcaataaa aatgatggta tcactatgaa gacttgtaac ataccaagtt ggtctataat
     9301 agcttgcgta tagccagata atttatttgc tgcaaatttg cctaaaaacc aaacacccat
     9361 taggaatgaa atatactttt caggcgaaag tttatttacc ataccatacc cgactggaga
     9421 aaagcaaatt tctcctatac tttggaatgt taaaaatcca actacccaaa atacacttgc
     9481 tttgtgaatt gaattagggt ctgttcctgt ttgtataact gctactatca taaacaaaaa
     9541 tgctatggct aatagtataa aacctaagct catttttttt ggcacactta aatcgccttt
     9601 tttagtttga gataatttta cccatactaa agcagagata ggtccaagaa taacacaaag
     9661 gagaccattg tatgaatcta tccatgaagt aggaatttga aaattgccaa cagtaagatt
     9721 tatgtgttcc tttgtataga gtgcaataga catagaggct tggttatatg ctatccaaaa
     9781 aaatactgtg aaaaaagcta acaccaatat tactattatt ctttttttct caatcaaagt
     9841 taaaggtctt gaagcgagca ttttcttata ttctagtgat tttcttatag actctctccc
     9901 gttaataggg tcattattca aaggtttata aaactttctg tttccaaata taaagaagaa
     9961 taaacctatt agaaccgtaa ttgatgaaaa taaaaataca tatctatatc catacatact
    10021 tatagataat ccgcttttca tagcaaacca ttcattagca actaaaccgc atattaaagg
    10081 gccaaataaa gtacctagat tagtagccat atacgtaata gagaaagctc catcttttct
    10141 tgggtcatca ttggtatata agtttccaac catagtatgt aagtttcctt tataaaaacc
    10201 tattcctaat ccagataaaa atatcatgat gtttatatcc atcttactat ttgctatgta
    10261 tcctagaaaa taaccaattg aaaccattag cattccaagt ataatacagt ttttgggacc
    10321 taaaaatcta tcactgaccc atccaccaat aactggagag aaataagtcc atgctaaaaa
    10381 agatgcaagc attgatgcgg attctgctgg tgttaaccct aaaccacctt tggagacttg
    10441 ttcagctaaa aacaataata ataccaattt cataccatag tatgcaagtc ctacaaaggt
    10501 aaatattgaa cagcagaaat ataaatctct aggttgttta agtttttctt gcgtttttaa
    10561 aactgacata tgtcgagaac ctccagataa atagtttaac aaaattttac catactatat
    10621 gagtatatgt ttttttcata tagacgtcaa cttgtaagat aatttaatta agtcgaaata
    10681 tatgtaaaaa tagtatcatt tttgtgtatt cagtaatatt tttttatgat aatatatgtt
    10741 attattatat taaaatacaa acatatttat ggaaaggata tgccaattta gtagttagat
    10801 tgattgtttg gcaggtggga tgagctatga taagaaagaa atctgaatta caagtagaag
    10861 taaaagaaaa tttaaatggt ggtataggaa aggttaagct tgaaaacata atacaaaatg
    10921 aagagcttaa aggaaaagga agactattta aaagagtagc tttaccagta ggaagttcaa
    10981 ttggtgtgca tgaccataca acagattttg aagtatatta tatactaaag ggaaaaggta
    11041 aagtatttga taatggggaa tttgtagaag taaatgaggg tgatgtagtg tatacagctg
    11101 atggcgaaaa acattctata gaaaatatag gagaagaaga tcttgagttt gtagcactag
    11161 ttttatatat gtaaaatatg gaggaggagt catgacaggc aagatagaag aaattaagga
    11221 caagataaat agattttgca actccaattt aaatcaagaa tacaaagata taagctttaa
    11281 aattttgcaa aaccttttag ataataataa agagataatt cattcttcta gagctgatat
    11341 atggtcttca gctatactaa atatagtttt agaacaaaat cttttataca ataagaagca
    11401 tccactacat ataacaaaaa aagagttttc aaaacaaata ggtgtttctt taaatactat
    11461 aaataataaa tcgtctttaa taaaagatgt ttgtagtatt gactttttaa atcaagatac
    11521 tatagctatt tctaatgtag aattttgggt aaaagagact aattccaaat caaagtctgt
    11581 agatttagat ttagaaaaga agaaaatact atataaaaga tatattaagc tatcacaaga
    11641 tgcgaatagt gatattgaat caattagata tatggaggaa gctgtaaata taggtaaaag
    11701 tatgattact aaagaagata aagaacttgg attttggaaa ggtttatcta ccagagccta
    11761 tatgatagca cttgaatccc ttgctagaaa gttagaaagt attaataatc taaaggagtc
    11821 caaaaaagta ttagaatatt taatagaaat taatcctagt gatgagcagg gaataagata
    11881 caaattatta aatgtattaa ttagattaaa tgatagaact tcaataaata aattatttga
    11941 actttataaa gaagaaaaat ctgctacatg gatgtacagt aaagctcttt attactttaa
    12001 aaataaaagt atgtttttgg caaatgattc tattaaggtt gctagaagta aaaataagta
    12061 cgttggatta tatttgttag attggcgaaa tgcttttggt agagaatttg ttacagagga
    12121 agaaaaggca gaagctgtct attactatga tgaaaacata gtgatttgga atgaagttaa
    12181 aggttctatg gattggctat taaaaaaaat gttggaattt tcataaaaaa aactactcct
    12241 tacttttata taagtcaaag gagtagtttt taatttatac atctagtttt aaatcttcag
    12301 gttttattag accttttttt ctcataaatt ctgaaatcaa caaagttaca atagcaggaa
    12361 gtataaaatt aagtaaaagt ataccaaccc acatagatat acctcctttt ccagcacttt
    12421 gcatagcagt tactgttcca aattgtccaa ccaatccaca agtacccata ccagaaccaa
    12481 taggaatatt ctccattttg aatattgttg tagataatgg tccaagtaat attgaagcta
    12541 cagtaggtgg tatccaaatt ttccaatttt ttattatatt aggtacttgc aacatagatg
    12601 tcccaagacc ttgtgccaca aggccaccaa ctccattttc tctaaagctc ataacagcaa
    12661 agcctaccat ttggcaacaa caacctacag ttgctgcacc accagcaaga ccacctaaac
    12721 ttaacatcat acaaatagct gcactactta taggtaatgt aagagctatt ccaacaagtg
    12781 cagagactaa agctcccatt attataggtt gcatagttgt agcttgcatt attatatttc
    12841 caaatgaagt cataaaagca gaaacagctg gaccaataag ttcagctaca agtactccaa
    12901 ttagtattgt tacagcaggt gtaaccaata tatctatttt tgtttctttt gaaactaatt
    12961 ttccaacctc tacagcaaaa agtgtagcga caaatgctcc aacaggtcct cctaattcat
    13021 taccacaagc tccaacaagt gcacaagaaa atagtacaag tggaggtgct tgaagtccat
    13081 acgcaatagc cactgctata gctggaccag taacctgttt agcaagaggg cttataactt
    13141 cagtaaaaag agaaatattg aaattatctc ctaatgtatt gaaaattgtt ccaattaaaa
    13201 gggacgaaaa aagaccgagt gccatataac ttagtgcgtc aataagatac ctttgaacag
    13261 aaatttcaat attttttctt ttagcaaatg atttgaaatt tcttttttta taattgattt
    13321 ccaatttaaa atcctcctca tataatataa gtataatttc tttttaatta attatataac
    13381 ttttcagctg acaagacaaa tgttatttca ctaaaattag actattgtaa tagtaagaaa
    13441 ttaacattaa ttttagtttt aaattttaaa tttatggttt ataattagta tagggatatt
    13501 ctgaaatata ataattttcc agtttatccc aatatttaac ttaggtagat tgcttacaaa
    13561 acaaatgatt atatattagg ctaaaaggaa gtggatagat gatatttgta aaaaataaga
    13621 aatacagaaa aattgccata attatatcta ttcctatatt gcttgttgcg cagtactacc
    13681 tttttaaatt tggaatactt agaccaatgg taaagggagt agaaattgaa attgtagatg
    13741 gggaatatgt taaagatata gataaatatg tagtaaaatt aaatgaaaca gttactttgt
    13801 ctacaggtga atatattaag gttccttcgt atgctaaaaa tccaaatatt tggtttaatg
    13861 tattggataa ctcagggact ttaaaaatag atggaaataa aatgacagca ttaaaggttg
    13921 ggtattcttc tgtgggtatc atgaaaaatt caagagtatt aaaaaaggct actataaaag
    13981 tagtagaccc agaggtagaa tcactagata tggagattaa tggggattta aaacatgttg
    14041 gtgatagtgc tactatagaa agtacaatag aagttgatta tgataagttt aaaaaatcat
    14101 ataagccagt ttataagagt agcaatgaaa atattttaaa aatcaaagga aataaagtaa
    14161 gtgcagttgg agttggtaaa gctacaatat atgctaagtg tggaaataaa gaaattgaga
    14221 gaacatttag gattcaagct agagttgctg aaataaatat ggatgatata aaaatagaag
    14281 ttgaccagaa tgaaaaattg aaaccagata taataacttc tccgcctggt ttagaaccac
    14341 caaaaatata ttatgagtta gtagattcaa aattacctgt agaaagagca ataagtttaa
    14401 gtagtaatgg aaatgttgta gggcttagag aaggtaaaga tagggtgaga ataacttgtg
    14461 gagataaatc taaagtaata acagtaacag tagtcaaaga aactataacg aatgactata
    14521 tacaaaactt ggttggttca cataaaatag taaacaataa agttattatt aaacttgaat
    14581 gggattatat gaaagatgtc tttgattatg atatatatct taaagataag ttgtcaggag
    14641 aatcatttaa gaaaattaaa tctataacta taaatgaaag tgatttaggt aagtctaaca
    14701 aggtatatgc aactatagaa gtagatatga agggtaatca aaatatagat tttgatattt
    14761 atgtcatagg gaaaaaggat tcagatacaa ctaagccaag taatgtaata aatattacca
    14821 atggaaatgg aaattcaaat aatgataata atattaaaga tatgagggta gaaaatatat
    14881 cagctaatgt tgatagagat aataatgtca ttagagttaa ctggagtagt atatctcata
    14941 aggactgtac ttacagtata tatgtaaaaa ataatactaa tggagatgga gaatttactc
    15001 tatatcaaag tgatatacag ggaaatgagc atactataag tattcctgaa ggagatttaa
    15061 atctagatat ttatgttgtt gcaaattata atggaaatag ctctcaaaat agtgatgtta
    15121 taaatgtaag atagatttgt atacaaattg aaagtgttga gtttatttaa atattaaatt
    15181 aagaaaaagg atagtatagg gtaattatac tatccttttt tgttacaata tataggttta
    15241 aaatctctag taatataaat tataaaaagt cttatttaca caaatctata gatatttttt
    15301 ttattaatca atattgtctt aaattgaact tagtgcggct tctattgcat gtattactgt
    15361 cgtatcaaat agtggaatag aagtgtcatt ttgtttaatt aaaagaccta tttctgtaca
    15421 tcctaatata acagcctctg caccttgttt gccaagctta tctataatag ataagaaagc
    15481 tttttttgaa gattcagata taattcctaa gcaaagttca ttaaatattg tgtcatttac
    15541 tattatacgg tcttcttcat ttggaattaa gacttcaatt ccattattaa taattacatt
    15601 tttataaaaa tcttgttcca ttgtatattt agtacctaat aaggccactt tttttatttc
    15661 tttttccctt aataccgtag cagttacatc tgctatgtga agtaatggaa tatgtatact
    15721 ttcttgtatt ttatctgata ccttatgcat agtattagta cagataacaa taaaatctgc
    15781 ccctgcctct tgtaatttaa ttgcagcatc agctaaaatt ttggcgcttt tttcccaatt
    15841 tccactagat tggcattctt caatttcttg aaaatctaca ctgtaaagta tacatttaga
    15901 agagtgtagt ccacctaatc tttcttttat tactgtatta ataacttgat aatatgtaat
    15961 ggtactttcc caactcatac caccaattaa gcctatagtc ttcatattat acctctctta
    16021 tttatttatt ttgaaatagc tatttacaaa tacaagtata gcatctaaaa tagaagcata
    16081 caatgataaa attgtatgga gtacaattat aattaagagg agtaattata tgccaataaa
    16141 ttcttttgaa aactatccta tgacatggaa accaaaatta gataaccgaa aaccaccaat
    16201 atataaaact ttagcaatgt tattagaaga agatattaaa agaggaaatt taaatcctgg
    16261 agataaactg cctccacaac gggaattagc agattttcta gatttaaact taagtacaat
    16321 aacacgagca tttaaacttt gcgaagaaaa gggactaata tgtgcaaaag tgggaaaggg
    16381 aacattcatt tcttctgatg taaatgtaag taatacatta ttatatcaaa cggaatcaaa
    16441 agacataatt gaattaggga cagttcatcc tccgtatgag caaaatacat atattattga
    16501 ctttattaaa aatgtactaa aacaaccaga aatggatagg tttttacaat acatgtctcc
    16561 atctggcaca tatatgcaaa aaaagtcaat tgcaaaatgg atagaaagaa ataatgtata
    16621 caccagtgaa gaaaatatat tattatctac aggagggcaa aatgctatat gtgcaacttt
    16681 actaggatta tttaaagctg gtgatagaat tgctacagat tctttaagtt tttctggaat
    16741 taagtcaata gctaaaatga taggtattca attagtccca attcctcaag aaaataatga
    16801 aatatcgatt gaatatcttg aaagttattg taaaaatgaa aatataaaag gcatatatct
    16861 tataccagat tatcataatc caacgacaca tactatgtct gatttatctc gtaaaaagat
    16921 agccaagata gcaaagcaat ataatataat aattattgaa gatgctataa atagcatttt
    16981 tagagatggt atacaaactc ccatattttc tttagctagt gataatacta tatatatttt
    17041 tagtacatct aaatttttat gtgctggtct tagagtagca tttgtagttg ctccaaaacg
    17101 atatatagaa aatttagaaa atgcactata taatatgaat ctaatggttt ctccatttac
    17161 agcagaaata gtacaccgtt tattatattc tcctattata gataaaatta tagaagagaa
    17221 aagagatgca attatagaaa gaaatgaagt tgctgataaa gttttgtcag attataattt
    17281 gattggtgat aagaattgca gttttcgttg gttacttctt cctgatgaat tggatggaaa
    17341 atcatttgaa atttctgcaa aaaatctagg agttcaagta tattgtgctg aacgattttc
    17401 agtaggaaac tctacagttc caaaagcagt tcgtatttgt gtaacagctc caaaagatgt
    17461 agaagaatta gaaaaagggc ttaatataat aaagtcactt cttaagagtt aaatttattt
    17521 tactattatt taattaaata aataaaaagg cggagaaatt tattataata aacctccgct
    17581 tttattattt aatgaaatac tttacttttt attaaaaaat ttaattttaa taatcttgaa
    17641 aatataatcg ttttatggta aaataattct taaaaataga aaaaattaat catttgttaa
    17701 aaaaaatgca taatttattg taatttattg acgaaggttg aaaaatattc ataaatgtgc
    17761 gaaataacag aaaaaatgca aaaattaatt caatttcatt tgttataaaa tctatatggc
    17821 aaaattaaat ctgcctatca gaatgatata aacacaccag aaaggaggac gctatcaaat
    17881 aatacaagta gttaactttg atagcaaaaa attatgaatg aacaaactag aatatcctta
    17941 gagagagctg ctgaattaaa atcaaaaatt gatgattata ttcaggctag aaaaacgatt
    18001 aacagaggtc ttgaaaaaga agaagagata aataaacgaa aacagaaaat attaagtatc
    18061 ttaaatggaa ctgaagagga ttggaataac tacaaatggc aattatcaaa tagaataaca
    18121 gatgtagata ctttatcaaa aattataact ctaactaaaa aagaaaaaga atatataaaa
    18181 gaggttggta ctcaatttag atgggcaata tctccatatt atttgagtct tatagaccca
    18241 gaagatacat gtgacccaat aaaattactg tctataccaa cacatataga gttggaagat
    18301 gaacaagaag atttggaccc aatgggagaa gagtatacaa acccagcagg atgtataact
    18361 agaagatacc cggatagatt aataataaat gtaacaaatg agtgtgctat gtattgtaga
    18421 cactgtcaga gaagaagaaa tattggacaa caagattctc ataagtcaaa agctattatc
    18481 caagaatcta tagactatat cagagaaaat gaagaaataa gagatgtact agtaactggt
    18541 ggagatgctc ttacattaaa agatgattat ttagagtgga ttcttagcca acttaaagag
    18601 ataccacatg ttgattatgt tagattaggt actaggactc ttgttacaat gccacaaaga
    18661 atcacagatg aattttgcaa tatgctaaaa aaatatcacc ctgtatatat aaatactcat
    18721 tttaatcatc caatggaaat aactaaggaa tctaaagaag cttgtgaaaa gttagcaaat
    18781 gcaggagttc cattaggaaa tcaggcagta ttattaaatg gaataaataa tgataaattt
    18841 gtaatgagat gtttaaatca agaattactg aaaataagag taaaacctta ttatatattc
    18901 caaagtaaac atgtaaaggg aacaaaacat ttcaatacat cagtagatga tggtcttgaa
    18961 atcatggagt atttaagagg atatacatca ggaatggcta taccaacata tatagtaaat
    19021 gctccaaaag gaggcggaaa gactcctttg cttcctcaat accttgtatc aaaaggaaca
    19081 gattacgtta tgcttagaac atgggaagga aaagttataa aaatggaaga tgaacctgct
    19141 gtagatataa agaaacttat aaaagaacaa gcgcaggatt aaaaaagtat ttaaagttta
    19201 taaaaagaaa caactttaaa ataaaaagaa aatggatgtc aaatttatat caatttgcat
    19261 ccatttttta ttagttattt attattttgt tgttgattaa ctaattcaag atatccactt
    19321 gggtggaagt aatatatctc agaagtattt tcatttacaa agtaaccaat atcaccatct
    19381 gcatctggta aaaatacata accattaaga tttttagctt ttaatgcttc aaaatcacta
    19441 gcatttcctt gatatatgta atcaacttca ttgttgtatt ttactaaaag ttctttagct
    19501 tgttcttgag tcaatttagt attttggctt acaggaacat tcttttgaga atctgcataa
    19561 gaagattcat ttactggtgc agttaataac aaacaagata aaactaataa agatataagt
    19621 gcttttttca tactaaaacc ctctttcatg tttaattata taagattcat tacttaataa
    19681 tattgtatgt ttcctataat taaatattac cattaaagag aataatatta cagttacaca
    19741 gctgtaactt ttgattacaa aatcgtaatt aaattgtatt tttattccat ttgtatttta
    19801 tcatgtctac gatttacaaa ctcacagaag tatatcttta taacgcccat cacaggagca
    19861 gatagaatca taccaggtat accaaagaat cctccaccaa cagtaactgc taggattgta
    19921 aagaatgggc taagacctag ttgtccacct acgatttttg gctctataaa tgtaacttca
    19981 acttgttgaa ttattattaa gtaaattaaa gaagttaaag caattgttgg attggagaat
    20041 aaattaatta ttacaactgg tgccatacct attactggac caaaatatgg aaccatattc
    20101 atgacaccca taagtgtacc aaataatagt gcatattcag attttaaaaa gtaaagacca
    20161 attccagaaa gtactccaac tataaaagaa tctaaaatct tacctgtaaa gtatttacca
    20221 atatttaaat ttaagctttg acatagttct attataaagt ctccatattt tttacctaag
    20281 gaaatataaa ctactttttt tgtaaacagt aagaattttt ctttttctaa taatatatag
    20341 aaacaaatta taaaacctaa aagaaattgt actataaatt ttccaactga aaatgtagtg
    20401 ctaaatatcc caccaagtga accaataagc aagttactaa atttaggcat tacactcata
    20461 attttgtcac catactcttt taatgtatta gggtcaactg attgtaaact cttgcctaag
    20521 tcgaataaaa actgctctgt tttatctatg tatgtaggaa tttgagcaac taaatcagct
    20581 aaactattta ctatggatgg aactgtaaat aatataaacg aaattagtat aactatgagt
    20641 acaccataag taagaagtaa cgaatatatt ctcttaaaat ttaatttact ttctaagaac
    20701 tttactagtg ggttgaatat atatgctaat acaaatgcta ttataaatgg tgttagtagt
    20761 gacattaata aacttatcac tccaaaaaaa tatttgtaat tatcaataac tttaatcaag
    20821 atatatgaaa caactacagt gagtacaata tttagataaa tttttttatc ttgcaaaata
    20881 acacaacctt tcttatattt attaaattta gatatactta aatctaagtt aaagttaata
    20941 tttatagctt attatacaat aaaaagcagt attaatatag tatgattaat atgaaattat
    21001 aaatacgtta aaactttatg tttatagcta aaataataaa aattatttat attgtgttta
    21061 tatataatct aaaatgaaaa aaagtggtgc ctttgattaa actaagtttt tataatttaa
    21121 attttgtaca ataagctatc ttttattatt ctttaaaata tatatttcat ttaattatga
    21181 caaataaaga taaaaattaa atattttaga ggtgatttta tttagatata gaattttaga
    21241 ataatactat acaaatgtat tgtattatta tttttatatc aaaaatagta ataaagtcta
    21301 tatacattta gaaaatactg tttaaaagga gtaggattta tgataaaaaa agagatatct
    21361 aactttagag gaagtagtga ttatgtagta tcaccagaat tgatggcatc agttaatgtt
    21421 gctattgctc ttgaaaagcc acttttgatt aagggcgaac ctggaacagg taaaactatg
    21481 ctagcacaag caatttcaaa tgaattaaaa aaagatttag taatttggaa tataaaatca
    21541 actacaaaag cacaagaagg gctttatgta tatgatactg ttcaaagact ttatgatagc
    21601 caatttggag gagaaggtgt agatgatatt agcaagtaca taaaatatgg taaactagga
    21661 gaggccttta gctcaaatca acaagtaatt cttttaatag atgaaataga taaggcggat
    21721 ttagaatttc caaatgactt gctttgggaa ttggataaaa tggagtttta cataaatgag
    21781 acaaaagaaa ctgtaagagc taaacaaaga ccaatagtta ttataacttc aaatgcggaa
    21841 aaagaacttc cagatgcttt tttaagaaga tgcatatttc attatataga atttcctgat
    21901 agagacatga tggaagaaat agttaaagta cattttgata aagtagaaga acacctattg
    21961 gaacaagtaa tgactacttt ttattggatt agaagtttaa aggacataca aaaaaagcct
    22021 agtacatcag agttaataga ctggatacaa gcattaactt tgagtggaat gcctattgaa
    22081 aaaatagaaa aagaagttcc ttttgcagga atactactta agaataatga ggatattgaa
    22141 tctatgcaaa gacacttata gttaatagga gctggataat tatgtttatt gaattttttt
    22201 atttattgag gtcaagagga ttaaatgtat ctttaaatga atggatgact ttaattgaag
    22261 cattggataa aggactgtgc tattcaaact tttcaaattt ttactatctt tgcaggatga
    22321 ttttaataaa aagtgaaagt gattttgata agtttgatgc agtgtttttg gagtatttta
    22381 aaggtataga acaccaagaa gaaattccag aagagattat gaattggctt aaaaaaccag
    22441 atatagattt agaagagttt gaaagattag aacaaaatcc aaatataaat cttgataaat
    22501 taagagctaa attggaggaa cgaataaaag aacaagattc tcctcataat ggtggtaatt
    22561 attggatagg cacaggaggc acttcagagt taggacattc aggaaaaggg caaactggta
    22621 tacgtatagg tggaaattct acttatggta gagctgttct tgaagttgca ggagaaagaa
    22681 aatacagaga ttttagagat gatgaagttt taaatatgcg tcaatttcaa gttgcactaa
    22741 agagacttcg tcaattttct actagaatag atgctccaaa gacagagctt gatttggata
    22801 agactattga agagacttgt aataatgcag gatatttaaa gttatttttt gagaagccaa
    22861 gaaaaaatac agtgaagctt ttattgctta tggattcagg tggttcaatg agagggtata
    22921 gcactttatg taatacatta tttcagtctg taagtaaatc aaatcatttt aaagatgtaa
    22981 agatatatta tttccataac tgtatttatg atagattatt tacaacacct gaatgttggt
    23041 taagtaaatc tattaatact gaatggatat taaaaaatat agataaaaac tataaagtta
    23101 taattgttgg agatgcttct atgtcgcctt ctgagttgct tcatattggt ggtaactatc
    23161 gtggtccata taattataca cctggtatag agtggcttaa aaggtttaag agaaagtaca
    23221 gtaaaattgt atggatgaat ccagaactta gagatggatg ggattcaatt agttattggt
    23281 atcaaactca aagaatgata caaagtgaat ttaatatgtt tccattgact gtaaaaggtc
    23341 ttgagaaagc attgaaaaat ttaatggttt caagataaag taaatataaa tgatggtatg
    23401 atttattcaa aattgtacca tcattattta tttttttaat atatctatta taaattgtat
    23461 aactgaaact taaaaaatta tatagtctaa tacattttag actcacacaa aacatataca
    23521 ttaataataa ttttagaagt cgaggtgatt ataataaatt atgaattaat tgtaaaatac
    23581 aatggagata tattgaggtt agaagaagaa ttaggtgtat cagtagagat acttaattca
    23641 tcatatgcaa taataacatc aagtaatgaa gaagatgtaa atatactttt aacttatcca
    23701 gaaattgaat ttatagaaaa gccttttata ttacagactc aggatgtgca aagtttttca
    23761 agtacaggaa taacaggatt taaaaataga actggtttaa ctggaaaagg aactataatt
    23821 ggtataattg attcgggaat agattatact ctacctgtgt ttagagatag tgatggaagg
    23881 tctaagatat tatattattg ggaccaatct attcaaggaa atccaccaga aggttttaga
    23941 gaaggaacat tatatactaa tgaagatata aataatgcaa tagatggaag tatgtatata
    24001 cctatttcaa caactagctt acatggaaca catgttgcag gtatttgtgc aactattgca
    24061 agtgatgcca gaataatagt ggttagagtt ggaaatatac agaccgatat tttttcgaga
    24121 agtacagagt ttatgagggc aattaaattt attttagata gggcattaga attaagaatg
    24181 cctgttacat taaatataag ttatggaagt aatgaaggtt cacatagagg aacatcttta
    24241 tttgaacaat atatagatga tatgtgttta ttttggaaaa acaatatagt tgtagcagct
    24301 ggaaataatg cagataaagg aggtcataaa aggattaggc ttcaaaataa tattacagaa
    24361 gaagttgagt ttatagttgg ggaaggagag cgtatattaa acataaatat atggccagat
    24421 tttgttgatg attttagtgt acatttggta aacccatcaa ataatcaaac acaagcaatc
    24481 tctctaactt caggtgagat aaggaatact ttgggtgaaa ctagaataac tggttatttt
    24541 taccctatag caccgtattc tctcacaaga agagttactc tacaattaag ttcaaataca
    24601 cagataactc ctgggttatg gaaaattgta tttgaaccaa tagatatagt aactggtaat
    24661 gtaaatatat atttgccaac ttctgaaggt ctaaatagaa atactagatt tctgattcct
    24721 actcaagagc ttactgtaac agttcctgga acagcaagta gagttataac agtaggtagt
    24781 tttaattcaa gaacagatat agtatctata ttttctggtg aaggagatac acaattaggt
    24841 gtttttaaac ctgatttatt agctccaggt gaagatatag tatctttttt accaggaggt
    24901 acaagtggag cattaactgg aactagcatg gctacacctc atgttacagg ggtttgttca
    24961 ttgtttatgg aatggggaat tgtaaatgga aatgatttat ttttgtattc acagaaatta
    25021 agggcattac ttctaaaggg agcaaggaga ttaagtaatc aatcttatcc aaataattca
    25081 tcaggatttg gttttttaaa tttatcagat atagatttgt acactctatc taatataaat
    25141 caagatttag aaacagagga tattggatat agaagtatta ataagtcatt taaagatgag
    25201 gaaaatagtt ataagtttat agatggttat aatatgcaaa tacataatga cttagagaat
    25261 gaaatttata taagtaaaaa tgcaagtaga caatcaggaa tagatatagt ccatactcct
    25321 gaatttgaag aagaactagc tgggttaggt atgagtcaaa ggttttttaa gataagtgat
    25381 tcattaggtg tactatctat aaataataca gactataata gcatacaacg agttttgcaa
    25441 cttccttcaa tcataagaac tgtatcgact acaaagatga cattacttgg agaaattaat
    25501 cgaggtactt ttggaggtgt agttgcaaca gaagaaatgg gcgtaaattt ctttaaaaat
    25561 aatcctaata ttaatataac aggaagaggg actctaatat caattgcaga tacaggaata
    25621 gactatctac acccagattt tatttatcca gatggcactt caaaaatagt atatttatgg
    25681 gaccaaacaa aagaaggaac tcctcctgat ggtttttata ttgggacaga atatacacga
    25741 gaagatataa atcgagctat cgcagaaaat gaccctagtc tatctcagga tgaagttggt
    25801 caaggaacta tgttgagtgg gatatgtgca gggcttggaa atgtaaatag tgaatatgca
    25861 ggtatagcag aagattctga gctaataata ataaaactcg gcaagataga tgatttttat
    25921 aatagtgcaa tgttgtttgc tgcatctcag tatgcatata aaaaagcttt tgaacttgga
    25981 agaccattag ttataaatat gtctttaggt accagtagcc tagcaggttt aaccaatagg
    26041 agtaatagtg aaaaagcgtt ctttactaga gggttatgta taacggcagg agcaggaaat
    26101 gagggcaata cacagacaca tacatctgga ataataccat atgttggagg ttctgttgaa
    26161 gttgaattag agttaaatga agacgaggaa gaattatcat tagaactttg gttaaataga
    26221 ccagataaag ctgatgtaat aatagtatct cccactggtg aagaaagtaa aagtgttgga
    26281 atttcaaatt ataataaagt aacaggttta tttgatttag aggggactga atattctata
    26341 acatatatat acccaacaac tttttctgga cagcaattta ctaatgttac tttaaagaat
    26401 gcaaaaagag gtgtatggaa gattcgatta gtaggagttt atattataac aggaagatat
    26461 aacttatacc ttccaaatag agaactttta aaaagtggaa ctagatttag agaggtagac
    26521 cctttttaca ctataaatta tcctgcgatt caagatgatt taataaccat aggagcctac
    26581 aataccatta atggtagttt atggcaaagt tcatcaagag ggccaactat tgaagacagg
    26641 ttaaaaccag atatagtagc accaggtgtc aatataatag ctgcttatcc tgggaacacg
    26701 tatgcaacta taactggtac agcagcagca agtgcacatg cagctggtgc tgctgcaatg
    26761 tattttcaat atacttttgt tgacggtaga tatccaaacc aagcatatgt gcaaaaaata
    26821 aaaactttta tgcaagctgg tgctagaaaa gattcaaaca cagtatatcc aaatacaaat
    26881 tctggatatg ggctcttgga tgttagagga atgtttgatg ttttaagata ggagggattt
    26941 atggaaaaat cttattgtat aatttatcaa ggtgatatag aatctgccct acaagaaaat
    27001 ggaataaata ggtatatggt tttaaatagt cagcttgcag taatatatgt acctttagat
    27061 tttgatgaaa ctattttaaa taatataata caagttgctt ggtgggaaga atctgaacca
    27121 atgagcagtc taattgaaat aactaataat gtaaataatg gagaaaccat tacaacagca
    27181 gctgaaactg attatattta tgaaaatcca tataatgata taacagggag aggaatttta
    27241 ttagcagtta tagattctgg gatagattat ttgcatcctg attttataaa tgatgatgga
    27301 actagtaaag ttttatattt atgggaccaa gaagcaaata caaatccacc tccagaaggt
    27361 tttatttttg gaagtgaatt tacaagaagt gagttaaata tagccataaa tagaaatgat
    27421 gggagtttga gtcaagataa tataggtact ggaactttag tttctggtat attagttgga
    27481 aatggaagga taaattctca gtacagaggt attactacag aatcagactt aatagtagtt
    27541 aaattaaaat catatacaga tacttattat gctggaagga taaattacag tgtttcagat
    27601 tttttagcag ctataacgta tgttactaac atagcaagaa cagaaaataa accactgata
    27661 ataaatttga ctattggagt taagtcaagt gctgtagcaa ctacttcaat actagatact
    27721 tttaatatat tgagtagtgc tggtgtggta gtagtgagtg gtgcaggaaa tcaagggaat
    27781 acagatattc attattctgg cagatttagc agtgtgggag aagttcaaga tgtaataata
    27841 caagatggag atgactatgc tcttgatata acactaaata cgaatggtcc agataaagtg
    27901 ggagcacaga taatatctcc ttcaggtgaa gttagtcatg atataaggta ttctccagac
    27961 ttttatatat atagaggaaa atttaactta gaaaatacta cttatgcaat gagatttatt
    28021 tatccatata ttacctctgg aaaagaaaat ttggagataa gattaagaga tataaaacct
    28081 ggagtatgga ttttaagatt gacttcagaa cttataataa atggagaata tgacatatac
    28141 ttgccaaata aaaatttgat agctccagac acaaggtttt tagagcctga ttcagtagct
    28201 actataacta tgtatgctgc tagtgatgat gtaattacag ttggtacatt taacaataag
    28261 actgatagta tgtggattgg ttcttcgaaa ggaccaatta gaggaagagg aattaagcca
    28321 gatatagtag cttcaggtgt agatataata tccacatata aaaatggaac atataataca
    28381 ggaacaggaa caggagtgag cagttctata gtcacaggtg ttttagcctt attgatggaa
    28441 tacttagaaa aacaggataa tgtaccgaga ttatcattgt ttacacaagt tttaaaaaca
    28501 tatttaatat tgggtgctac aaaacttgag atatatacat atccaaatgt atcgcaggga
    28561 tatgggattt taaatttaaa aaatacgatt caacagatag caaatactct atagataaga
    28621 acctatgtaa aattataatt gattattagt ttaattgtat caaatacttt agatagagta
    28681 gaaaaatagc ataaaaaata aatatgaaaa ttaaaaaaca gttttatatt ttgaatacac
    28741 aaagtaaata ttttgtatat aaataggtat aaattaagta ttgaaaagat attatgttaa
    28801 tttaaaaata attataattt agacttatga attttatttg taaaattgct aaaactatta
    28861 caaaataaat aatagaggtg atagttttgg aaagtaaaaa ggcaagagca ttaaaaggta
    28921 aagataataa aagacttaaa agttcacttt tagttggaaa tgagtcgaca gcagcatggg
    28981 ctgatgttga aaaattaaaa cctcatagta aagtttcaat accttcaatt aagggtgtcg
    29041 aagaagcaaa agactgggtt gacaatggaa gtaaattata aaattttgga aattgaagta
    29101 ataaagaagt aatgaagata agtatttatt atcattatgt aaggtagaat tattatattt
    29161 taaataacaa tcagtatcaa ttatataaat ttattgacta aaaatttata aattggtata
    29221 attttgttaa ttagcagaaa taataaaaga taaatactgt gaagagaaga gtagatataa
    29281 aatgtagttc tagcgagttg aggatggtga aagctcaata cgaggtttat attgaagaac
    29341 atcttggagt ttctaatcga aagcttattt agtaagtaga cttagacgga aggcaccgtt
    29401 ataggcttta agcattgctt aactaagagg tcattatgac aactagggtg gtaccgcgag
    29461 agtaatagtc tttcgtccct ttatataggg atgaagggct ttttttattg aaattatatt
    29521 taaaagaaat taataacaat tatactagga ggatttaaaa tgcaaaagga atttataaca
    29581 gtaaaagaat tgtacagaga aaaagaacag tacataggga agactgtaaa agtagctgga
    29641 tggataagaa catctagagt atctaaaaat tttggattta tagagctaaa tgatggaagc
    29701 ttctttaaaa atatgcagat agtatttgat gagaaagtag aaaattttaa agaggtagga
    29761 aaattagcaa taagctcatc tatacttgta gagggagagt tagtatctac agaaggggct
    29821 aaacaaccag tcgaaataca tgcaaaaagc atagttgtag aaggggaatc agatagttcg
    29881 tatccactac aaaagaagag acatacgctt gaatatttaa gaacgatagc acatttaaga
    29941 ccaagaagta atactttttc agctgtattt agagtaagat caatagctgc ctatgcaata
    30001 cacaaattct tccaagaaag aaactttgta tatgcacatt ctccaatcat aacaggtagt
    30061 gactgtgaag gtgcaggaga gatgtttaga ataacaacta tggatttaaa tgatattcca
    30121 aaaactgaag atggcaaaat agactatact caagatttct ttggaaaaga ggctaattta
    30181 acagttagtg gtcaattaaa tgccgagata atggcacttt cttttagaaa cgtttatact
    30241 tttggaccta catttagagc tgaaaactct tatacaggaa gacatgcttc agaattttgg
    30301 atgatagagc cagaaatagt ttttgcagac cttgaagata acatggaact tgcagaagat
    30361 atgataaaat atgttataag ctatgtatta gaaaatgctc ctgaagaaat ggaatttttt
    30421 aatagtttta tagataaagg attgttagaa agattaaata agatagtaaa ctctgagttt
    30481 attagaataa cgtatactaa agcagtggaa ttattaattg aatctgggca tgaatttgag
    30541 tatcctgttg agtggggatg tgaccttcaa acagagcatg aaagatatat aacggagcaa
    30601 atatataatt gtccagtgtt tgttacagat tatccaaaag atataaaagc tttctatatg
    30661 agaatgaatg aagatggtaa aactgttaga gcaatggatt tactagttcc tggagttgga
    30721 gaaatcgtag gaggttctca aagagaagaa agagaagatg tattgttaga tagaattaat
    30781 gaaatgggat taagagaaga agattattgg tggtatttag aacttagaaa gtttggtact
    30841 gctactcact ctggatttgg tcttggattt gaaagaataa ttatgtatat gacaggtatg
    30901 tctaacataa gagacgttat acctttccca agaactccta aaaatgctga attttaataa
    30961 ttatatataa taaaacttat ataactttaa gtataaacac taaaatgact tgcatattta
    31021 tctattttag agatatgcaa gttattttta ttgtactata aatatataat ttgattactt
    31081 ttattttgcg atataatact tataggttta tgtaatagta tgtaaccttt taataaaatg
    31141 caacacaatt acgtccttgt tcttttgcag tatatagggc cttatcacaa ttacttataa
    31201 actcatttaa tgttattgtc tttccaattt tctttgtata aactccaata ctaacagtta
    31261 cacattttaa aggaacatca tcacgctcaa aattgcagga ctctataatt tttcgtaaac
    31321 attctgattt aagtttagca tctgcctcat tacagttaaa aatacatata aaaaattcct
    31381 caccaccata tcgtatagca tgttcatttt cagaacttgt aaaatctaaa attactttgg
    31441 caatattttg tagacaatag tctccctttt gatgtccata attatcatta aattgtttaa
    31501 aataatctat atctatcatc attccagtta taggttcttg atttgcatgg ctgattaatt
    31561 ttttttgtag aatatcttca aggtacctac ggttgttcat tttagtaagg tcatctgtca
    31621 taactttgtc gttaagtaaa ttatttatat gagatatttc ttcatattgt tgactaatta
    31681 aaagtttatc atagtaagta aatacacgat ttctatatag cattgtagaa acaaaaatgg
    31741 caagggatgt tataaaaaat gtgttgacaa tattactaaa ctcattgcct ggtatagttt
    31801 gaatcccagg taagaataaa ttaagaaaca aaaagcatcc tccaaaaact attactgatt
    31861 ctccaaattt taatactgag aaagctgcag ctgctaacat aatataacta aatacactta
    31921 ggtcatttcc acctaactgg tcaagtagag taattacaca accccatagg cagaaaaaag
    31981 tcacataaat tatccataaa tttatataaa acgtacagca atcctttttt ttattccaaa
    32041 ggtaagaaaa tacaaacata aagacagaag atacacaaaa tagtgttaca tacatataaa
    32101 aataccattg gcgtcttggt gtgacaaaag ggcctccttc tctagctgag attgatatca
    32161 tcataattat ttgagatatt actattatta tgcaaatgat tttacccgta tgataaagat
    32221 aattgcagag ttctttctca acattgtctt tgtgtttttt gtctagagga aatccaaaaa
    32281 atttttttat tttttctaag ttcatataag cctccattaa aaataaagca taaaaataag
    32341 tattcctttc tatcattaga tatttggaat acttcgttta tgatttaatt atatcaaaat
    32401 atattgtgtg taacaatcta aaattgtgat agataaaatt tattagataa tgtcgagtaa
    32461 ctttattgat taatacttta ttataattaa gatttacaga tagtttacta ctgaaaataa
    32521 agaatagaat aaatttaaat taagcataat aaaatataaa taataaagtt cgcattaaca
    32581 aactatttaa atatcaagac ctgttaaaaa acaacagcaa taaacaaata aaatagataa
    32641 atatatccaa ttaaggtata attaagtttt atatgatata gttatatttg ataatttaca
    32701 atagtaaaac ttggagggaa aaaatgggtg aaaagaaaat aaataatatg aattattcac
    32761 ataataatta ttccaaaaag acaggtgaac caactagaaa aataaaaaat agccatatca
    32821 ctcttaaaag taaagaagaa aaaatttgtt ctagttgcaa taaagaaaat aatactaagg
    32881 ataattactg taaattttgt ggaaatgaac tttatgaaat agcatcatta agaccacttg
    32941 aaacaaaact agacttgaaa tctaaaataa aagagctttc atatcatgca aataaaagag
    33001 gtgtattttt aacaacattt acaaccatat ttatattgtt tattattgca ttgatattta
    33061 aagctattat aactattcaa tttaatgata ttagttattt aataaatcca gcacatataa
    33121 tcctagctct caacttgggt caaattagtg tatctatgtc taccatgatg ggttctgggt
    33181 ttataaatgc caatataggt ttgttgatat tacttataat ccctattttg gtattattaa
    33241 tatcaaacct aatttttatg agaaaaagat gtagagattc aaaaactgta ttagcaaatt
    33301 ctctaggagt tggaattttt tatggattag ttttagctat tttatctatt tttactaatg
    33361 taaaaactag ttcacatagt atgttagaat atggatatgc attagaatat agttatgaat
    33421 tttttagtgt tcttttaaat ggatttgtat tagggtttat atgtacatac ataactactt
    33481 ataaaaaaag ttatgaaaag gaaaatatgt atttatctct tttttcaaat tctataagaa
    33541 cattcatatt aggttatgtt ttagtattag taatactatt ggtactaact atatctgata
    33601 gtagttactt aaatgaacta gatatgagta gttattcaaa tggattaaat ttatttacta
    33661 tactcccaca aatagctagt tatatgtggg cttttgcaaa tggaatttca gtaaccataa
    33721 taaactcaac agtatcaatg tttactttaa gttcttcatc tttatttgga gatactaaat
    33781 taatgtttta tgcaatggga gcattgtcta tgttaatatt attattaaat ggatacaagc
    33841 taagatttaa gtataataca gatagtataa gacctataat agtatttagt atatattatg
    33901 catttttgat gggaatattg gctttattta gtacatttat actagatagt aatattaatt
    33961 tctttaatac aactaattat ggaacaactt taataatgca atttaaagtc ttacaagcta
    34021 ttgtaatttc atttgtatat agttttgtga tatctttaat aggatataag ctaaattcag
    34081 ctgattaaat atgattggag ggaatttctg tggaaggaaa tactaataat aaagtagatg
    34141 ataaattttc taaggttaag aaaaagtttg gtagtggaaa atttaaagtt gaattatata
    34201 aagaaaaatc aaaattaaat agagagattg atgatattca agaaaagaaa gcaaagatat
    34261 tcttggaaat gggaatactg acatatcaaa aaattagaga agattctatt aatgataata
    34321 gttttgataa attctgtgat gagttactag aacttgataa gttaatttat gaaaaaaata
    34381 tggaaattaa tgaaatggaa atggcagaaa gtaatgtaac ttgtgagtgt ggttatgttg
    34441 gaaatgtaaa tgataaatat tgtgctgaat gtggtaaaaa atttgaattg gaaaatgaat
    34501 gtgatgattt tataatttgt ggttattgtg aaagtgagat agatacagag gctgaatttt
    34561 gtccatgctg tggaagaaaa attataatgg atttagaata atattgggaa acctatgtat
    34621 agtatacatg ggttttttta ttgaaattat ttaaatggac ttataacagt agttatataa
    34681 aatgaaaata aacaccaaaa aaatgaaata aaatttcttt taaatgtaaa aaacaagcta
    34741 atatataaaa taaatcagta aaaatataga tgcgagaaaa aaatatgaaa aaatattgca
    34801 aagaaaaagt tttcatgtta ttattaatac ataaatcaag aaaataaatt tcagaaacga
    34861 ggtaatttaa aaaatgctag ataaggtaaa gggaagatta atagtatcat gccaagcttt
    34921 agaagatgaa cctcttcata gtccttttat aatggggaga atggctaagg ctgctatgga
    34981 aggtggagct gttggaatta gagctcaagg agttgaagat attattgaaa taaaaaaagt
    35041 tactggactt cctgttatag gtattataaa gagaaactat gaagattcag atatatatat
    35101 aacgccaact aaaaaagaag ttgatgaact tttaacaaca gggtgtgaaa tgatagcatt
    35161 agatgctacc aatagagtta gaccaaataa tgaagactta aaagaactta taaagtatat
    35221 aaaagaaaac ggtgttctag taatggcaga tatttcaaat tatgatgagg ctatcaaagc
    35281 acaagagtac ggtgtagatt gcgtttcaac tacactatct ggatatactc catatactaa
    35341 aacattagaa ggtccagatt ttgtattaat ggaaagatta gttaaagatt tagaaatacc
    35401 tgtaatagct gaaggaaagg taaatactcc tcaagattta aaaaaggtct ttgagttggg
    35461 tgttcattca tcagtagtag gttcagcaat aacaagacca caattaataa cagaaaaatt
    35521 tgttaaagca attgaaatta attaaaaatt aatttataaa ttaaaaaatt attatattta
    35581 actatatttg aaaattaaaa ttggaggtaa agatatgaga acaacagata tgaagggaat
    35641 ttattcagca ttattagttt catttgataa ggaaggaaat ataaatgaaa aagggttaag
    35701 acaaataatt agacacaata tagatgtttg taaagtagat ggtctttatg taggaggaag
    35761 tacaggggaa aacttcatgc tttcaactga tgaaaagaaa agaatatttg aaatagctaa
    35821 agatgaagtg aaagaagaaa taaaattaat agctcaagtt ggttcagtta atttaaaaga
    35881 agctgtagaa cttgctaaat ttacaactga tttaggatat gatgcaataa gtgctgttac
    35941 acctttttac tataaatttg attttgaaga aataaaacac tattataata caataataaa
    36001 ttctgtagat aatagattaa taatatactc tataccattt ttaactggtg ttgatatgag
    36061 tttagaccag tttggagaat tatttgaaaa tgaaaaaata ataggtgtta agtttacagc
    36121 agcagatttc tacttattag agagaatgag aaaaacattc ccaaataaat taatatttgc
    36181 aggatttgat gaaatgatgt taccagctac agttttaggt gtagatgggg caattggttc
    36241 tacattcaat gtaaacggtg tcagagctag acaaatattc gaattaacta agaatgaaaa
    36301 aatatcagaa gcacttgaag tacaacatgt gactaatgat ttaataacag atatattagg
    36361 aaatggctta tatcaaacaa ttaaattact acttgaagaa caaggtgttg aggctggata
    36421 ctgtagacaa cctatgaaag aagcaactga tgaaatgaaa tcaagagcaa aagaaatata
    36481 cagaaagtat ttttaataaa atacttttag tatgggagga ttagtcaaat ggtaggattt
    36541 acttggatag attttgcaat actagtggtt tacttattgg cagttttatt tgcaggattg
    36601 ttattttcaa aaaaggaaat gaagggaaaa gagttcttca aaggtgatgg aactattccg
    36661 tggtgggtta catcagtatc aatatttgcc acattattaa gtccaatatc atttttatca
    36721 ttagcaggaa attcttatgg tggtacttgg attttgtggt ttgcacaatt aggtatgttt
    36781 atagcaatac ctttgacaat aaaatttttc ttacctctat atagtaggtt agaaatagat
    36841 acagcatatg aatacttaga aatgagatac gaaagcaaag gtcttagagt acttggagct
    36901 ttgatgttta taatatatca aataggacgt atgtctatca taatgtatct tccatcaata
    36961 gttctggctg gtcttacagg aatacctgta aatgtactga taatagttat gggtgtaata
    37021 gcaataattt actcatatac aggtggatta aaggctgtct tgtggaccga ttttatacaa
    37081 ggaatggtat taatagtagg tgttacaggg acattgttct atttaatagc aagtataaat
    37141 ggtggttttg gaacagtaat ggatactctt acaactggac acaagttctt agcaagtaat
    37201 gaagttatgt ttgataaaaa tatactatct acaagtgcat ttataatatt tgttggagct
    37261 ggtcttaata ctttttcatc atatgtatcg agtcaagaca tagttcaaag atttactaca
    37321 acaactgata taaagcaact taataaaatg acatatggaa atggtgtttt atcaatggga
    37381 cttgcaacag tattttatct aattggaact tgtttattta tatactatac tcaaaaccca
    37441 gatttagctc aaacagttca acaagaccaa gtatttgcat catatatagc ttatgaatta
    37501 ccagtgggta taacaggaat actacttgca gcgatatatg cagcttctca atcaacttta
    37561 tcgacagggc ttaattcagt tgctacaagt tggactttag atatacaaac tatcataaca
    37621 aaagatataa gtatggaaaa gcaaactaag atagcacaat atatatcatt aggtgtaggt
    37681 atactttcta tagctgttgc aatagtatta gcaaatggag aaataaagtc agcatatgaa
    37741 tggtttaata gttttatggg gctagtacta ggtgttttag caggaatatt tgtacttggt
    37801 gcattttgta aaaaagcgac taaaatggga gcatatgttg gattcatagt atcagcaatt
    37861 ttagttgttt atttaaaata ccgtatgcca gaagtaactt cttgggcata ttctctaata
    37921 acaatcacga cttctgtagt ggtaggtaca atagttagtg caatagaagc taaagttaaa
    37981 gcaaaagtat ctttaccaaa agctgagact actgtttatt atgaaaaata atatttaaat
    38041 gataaattgt cttatagagt gattagataa ataatttagt tactctataa gattgtataa
    38101 tatactttgt atataaaagt ataaaaatag actttataat tatataggga gatagtgctt
    38161 atgatatttg gaaatataaa tcatagtaag acatattcaa cattgcatga agatttactt
    38221 agatgttttg aatatgctaa agataattca ttaatagatt atgaaaaagg tacttataca
    38281 atagatggtg atgatatttt tgtgaatatt gtagagtata aaacttgtga aaaagaagat
    38341 agattctggg aagctcataa gaagtatata gatgttcatt tgatgcttga tggatgtgaa
    38401 cgaatagata taaattttat agaaaattta gaacaaaaaa tatatgaaga aaagggcgat
    38461 ttcttgcctc ttgatggtaa aaataatgga tacatggaac ttagaaaagg tgatttttta
    38521 gtttgttatc cggaagatgc tcatatgaca ggaataaaag ttttagaaaa agaaaatatc
    38581 aaaaaagcta tttttaaggt tttggttaga taatgataat ttatataacc actagggctg
    38641 aaaatagtga tttaactttc agcgattagt gagacttcgt aaattttagt tagattacta
    38701 ttttcagcct tagtttttta tgagaaaatt gaaaataatt aaaagcttaa aattttgtaa
    38761 aggagttaac ttatggaaaa tatagatgta ttcttattaa taggacaaag caatgctaga
    38821 ggattgggaa atccaaaaga gagcatcatt ccaaatgaaa attgttttga atatttgagt
    38881 actgatgaaa ttataaatat gagatgtgag ctagagactt cagaaggtga tggtactata
    38941 gcacctgcat tttcaaatga atggaataag cttactggaa ataaagtttg ttttattcac
    39001 aatgcaaaag atggttcaag aataaaaaac tggaatcatg ataataactg gtttctaaat
    39061 gacactatag aaaaatttaa tacaggctgt actaccttaa gtaaacatta taatatagaa
    39121 aataaatacg ttatttggat tcaaggtgaa agtgatgcaa aatatggtag tgatgtattg
    39181 tactataaag aaagtctaaa aaaaattgcg tataggctta aagaagaatg tatgattgat
    39241 aaaatgtttg taagtttaac tggttattgg cttggagaag atgaatattt tattagaaca
    39301 agacgaattg cagcagcaca ggagtcggct tgtagtgaat gtgatatatt atgtgtagga
    39361 agcaaaattg ctatgcagtt tcatgacaga ggattaacta ttgatgatgt tcattacact
    39421 caagaaggtc ttaatatgtt aggagaggac ttatgtaaaa atatttataa gtatcatata
    39481 accaaagaaa aaagtgtatt aagtgatact atagacttat cagaagcaag gaaatatatc
    39541 tgtgaattgg aaaagataag taaaagattt gtgtaatata aaattttaaa tagagttgtt
    39601 aatttataaa aataatttgt atggagatat gttatgaaaa attacgtttg tatagatgtt
    39661 gggggtactt caataaaata tggttttatt agagaagatg gatttataat tgataaatca
    39721 agtcttgata ctgaagcaaa ggaaaaaggt ggagaaggaa tattagccaa aataaaagat
    39781 atagtaaaaa aatatataga ggaaaatgaa ataagtggta tatgtatatc aactgcaggt
    39841 atggttgacc cagttgaagg taaaatacta tttgcattag aagaattaat tccaaactat
    39901 aagggcatgc aattaaaaaa agaagtagaa aaagagttta atataaaatg tgaagttgaa
    39961 aatgatgtta attgtgctgg acttggtgaa atgtggcttg gagcaggtaa agatgcaaca
    40021 tcttctatat gtttaacaat aggtactgga attggtggat gtattataat aaataataaa
    40081 ctgataaatg ggtttagtaa tagtgctggt gaagtaggat atatgaatgt aaatggaagt
    40141 tcttttcaag aattggcatc tactagcagt ctaattaaaa aagtagcaaa aataaaaaat
    40201 ttgaatgaaa atgacttaaa tggaaaaata atttttgatt tagcaaagaa taatgatgaa
    40261 gattgtttaa aagaactcga taatatgata aaatcattag cagttggaat cgcaaatata
    40321 tgttatgtaa taaatccaga agtggtaatt ttaggtggtg ggattatggc acaagaaaag
    40381 ttcctaaaac caaaaataga caaagcatta aaggaggttc ttatagagag agtttacaaa
    40441 aacactaata tagaatttgc aaagagacaa aatgatgcag gaatgcttgg agcattatat
    40501 aattttttaa acaaaatata aagaatagaa gggtttttaa tgagtatttt agaacaattg
    40561 gaatcaccaa cttttaaagt cacaaaatca gataaattat taatagctta tataaaagaa
    40621 aatatagatg atgtatttta taaaccaata tcacaaatag ctaaagagag caatattggt
    40681 gaagcaacta taactaggtt tgtaaaaaaa atgaatttta atggattaca ggattttaaa
    40741 gttactttag cacaagaaat atcaactatc aataaaaaaa atattataaa taaaaatata
    40801 caaaatgatg agccagcttt ggatacagca aaaaaacttt taagttcaaa tgtaaccact
    40861 ttagagaaca cagtagagat aatcaatagt aaggatgtac atgactgcgc aaggcttata
    40921 ataaatgcaa agaaagttta cttcataggt attggatatt ctggtataat agcacaggat
    40981 tcaaactata aatttatgag aataggtctt aattgtgtaa gttttgacag tagtcataca
    41041 atgataatga tgtcttccat aatggaagaa ggagatttaa taattgcaat ttcacattct
    41101 ggtgaaacag aagagattat aaaaactgta aagcttgcaa gagccaataa tgctaaaatt
    41161 atatcaatta ctgaaaataa aaattctgaa ttaaaggata tttcagatgt acacttatct
    41221 tatgtatcgg gagaaacagt actggaaact ggttcaatat cttctaaatt agcacaattt
    41281 ttcattattg atttagtata tacacaggta gtaaaagaac tttctaatga agctattgaa
    41341 aggaaaatta aaactacaaa tgctataaaa ttgtttaaaa atgaataaaa taagaaattt
    41401 gtaatgtttt atagagctct cttaaaaatt taggaatgga gagatattac atgtctaaat
    41461 taaaagatta taatattgat aaagtaataa tttttaataa tttaagtact aaaacgaaga
    41521 atcaacttaa agaaaaagtt aaactaaaaa gattacaaaa aaaagaaatt ttattttatg
    41581 aaaaagatag cttagacagt gtttatatac ttttagaagg aaaggtatct atttttaaga
    41641 taagtgaaaa tggagagaga aaagtaattt tcatattaaa tagtggggaa gtcataaatg
    41701 atattacact tgacgcttct aaaaactcta ctgttggctg tgaagcattt gaaaattcaa
    41761 ttgtagctta ttatagtatt gaagaatttt tagaaattat gcaaaatgat tttcaactta
    41821 caaaggatat tatttcttat atggaaagaa gaataagacg tttatataga cagttgaaaa
    41881 attctatatc cataaaagta gataaaaaac ttgcagcaaa attatatagg ttaagtagag
    41941 aatttggagt ttgctgtgga gaatggactt tactaaatgt aaatattaca gttacatatt
    42001 tagctgatat gcttggctgt aaaagggaga ctgtttccag aatgataaag gtattgcaaa
    42061 aagaagaact tattaaaatt gacaataaag gtttttatgt taaagaaact gaattatcaa
    42121 ggtattttaa aaataattaa ttatgattaa gtaagagtat tat
//
LOCUS       DAYAPA010000021        41423 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.021, whole genome shotgun sequence.
ACCESSION   DAYAPA010000021 DAYAPA010000000
VERSION     DAYAPA010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 41423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41423
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.021"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(64..2034)
                     /gene="ftsH"
                     /locus_tag="ACGI59_001009"
     CDS             complement(64..2034)
                     /gene="ftsH"
                     /locus_tag="ACGI59_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFG9738813.1"
                     /translation="MNKLLKGAGFYLLVFIIIVGIVQFSGKPTEKIKDLKFSEVYREL
                     TDENISRLYFVNQTSVEGTIKDTNTKFKSYVPTEVMGNKLADEVLDQAKAGKLTFGGE
                     AKPSTPWFVEMLPTLLLIFFMVIIWFVFMNQSQGGGGKVMSFGKSKAKVHKDDEKTRV
                     TFKDVAGLDEEKEDLQEVVDFLKNPKKYIELGARIPKGMLMVGPPGTGKTYLSRAVAG
                     EAGVPFFSISGSDFVEMFVGVGASRVRDLFEQAKKSAPAIIFIDEIDAVGRKRGAGLG
                     GGHDEREQTLNQLLVEMDGFGVNQGIIIMAATNRPDILDPALLRPGRFDRQVVVGTPD
                     VKGREAIFKVHSRNKPLSDDVKMDVLARRTPGFTPADIENLMNEAAILTARKREKKIK
                     METIEEAITKVIAGVAKKSKVISEKERRLTAYHEGGHAVCAHVLEEVSPVHQVTIVPR
                     GRAGGFTMQLPVEDKFYATKNEMKENIIVLLGGRVAEELVLKDVSTGASNDLERVTAT
                     ARSMVTKYGMSSKLGPMSFDSDDEVFLGNSFSSKRNYSEEVAFEIDQETKRIVDGAYD
                     KTRSILQENMDRLEYVAQALLIYETLDAEQFVKAFNKELPLNEIENAVTEENSSKEVE
                     EQLTIKLEKDEEERNNVIDINKNLEDKSDKDK"
     gene            complement(2068..3456)
                     /gene="tilS"
                     /locus_tag="ACGI59_001010"
     CDS             complement(2068..3456)
                     /gene="tilS"
                     /locus_tag="ACGI59_001010"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFG9738814.1"
                     /translation="MIFDKVLSTINKHNLIQKGDKIVLGLSGGPDSVCLLHVLNRLKK
                     DFNIEIYAAHLNHQIRGIEAQKDALYVSKLCEDMGIIFFVKSINVPKYCENEGLSLEE
                     GARKLRYEMFYEIKDKIKANKIAIGHNLNDQAETVMMRIMRGTGLKGLKGIDYIRDNC
                     IIRPILDVERNEIEEYCEAYNLNPRIDKTNLENIYTRNKIRLDLLPYMKDNFNSNVIE
                     SIVRMSNSLKSDNDYIEKEAEAKFREVSNIKEKGFVEINLDDFVCLHDAIKVRVLRNS
                     IKHILGDTNFVDQRHIEDIMSLEDNSKVNKMLTLPRNIFVYRKKDSIILTNEEIVNEE
                     IEFYYNVPSNGFIKIKELKQIIETQVMSIDRYKSMKLDNSSKGFDFNKVKGGIVIRSR
                     RQGDKIKLAMGSKKVKDLFIDLKIPREERCKIPIITDGEGIICVGDYKISENYKIDES
                     TKEVLKINFNKL"
     gene            complement(3639..4067)
                     /locus_tag="ACGI59_001011"
     CDS             complement(3639..4067)
                     /locus_tag="ACGI59_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HFG9738815.1"
                     /translation="MEEKSLSVKISINTRQYDEKGNMDTIEMTAFGKIFYKNDGIYVI
                     YKEKEEDIEITNTIKILKNQVSIKKFGAINSTMLFKCGESSTTKYVTPQGTLLIDIDT
                     RELDINIQEGEHIKLKIDYNIKVQDLFLGRNKIDIYIGTK"
     gene            complement(4084..4890)
                     /gene="murI"
                     /locus_tag="ACGI59_001012"
     CDS             complement(4084..4890)
                     /gene="murI"
                     /locus_tag="ACGI59_001012"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFG9738816.1"
                     /translation="MNNKPIGVFDSGLGGLTVLKEIMKILPNEDIIYFGDTARIPYGS
                     RSKETIIKYTFQAINFLKTKGVKAIVIACNTATARSLKEAQEKYDIPIIGVIEAGART
                     AVSSTKNKIVGIIGTEGTISSKAYNLEISKIDESIEIVNKACPLFVPIVEEGWANTEV
                     AKLTAKIYLQELKEKNIDSLVLGCTHYPILKRTIGEEVGEHIKLVNPAKETAKDLKKI
                     LEVQNIINNTEIHGTYQYYVSDIPEKFSDIAREFLKKKIDKIQKVEIQKY"
     gene            complement(5092..5598)
                     /locus_tag="ACGI59_001013"
     CDS             complement(5092..5598)
                     /locus_tag="ACGI59_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HFG9738817.1"
                     /translation="MDRWSKLMATLLALTFIFMPIKEVFAYEPTENIKKVNKQEKNIA
                     KKEVINLSDNDLMLLSKLVAGEARGESYEGQVAVAAVVINRVLDPRFPDTIEEVIYQK
                     NAFSVVLDGSIKMAPTDSARKAAQEALYGTDPTNKAVYFWNPEIATCKWINRLNPYMR
                     IGNHVFAK"
     gene            complement(5628..6287)
                     /gene="spoIIR"
                     /locus_tag="ACGI59_001014"
     CDS             complement(5628..6287)
                     /gene="spoIIR"
                     /locus_tag="ACGI59_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein R"
                     /protein_id="HFG9738818.1"
                     /translation="MRKIKIRLGLLILSLISVISIMTIVINGEVKKVDNISKDYKDKL
                     IRFHVIANSNTDEDQELKLKVRDEVIKYLQPKLQNSKSIKESEAIIKKEYSSLEEISK
                     NIILKNGYNYSVKVGIQYSNFPTKQYSNIVLPAGEYKALKIIIGKGEGKNWWCVMFPP
                     LCFVDESNGVIDKSTDDKLKEVLTDKEYKLIKQDTPKKTSRVKIKFKVLEVVKDLEKK
                     F"
     gene            complement(6432..7112)
                     /locus_tag="ACGI59_001015"
     CDS             complement(6432..7112)
                     /locus_tag="ACGI59_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFG9738819.1"
                     /translation="MENLTKLNLDNYKPLRDVVFENLREAILEGKLKPGQRLMEVQLA
                     EQLGVSRTPVREAIRKLELEGLVVMLPRKGAYVANMSLKDIMDVLEVRASLEGLAAYL
                     AAERISDEDIKKLKDISEEFKKSTLESDVDALLKLDVEFHECIFKATNNKKLIQLINS
                     LWEQVYRFRVTYMSAYDTSHNIVEEHKYLLDAIIKGDSELSKKYATEHIEKAEQFMID
                     KAMISKDL"
     gene            complement(7096..7986)
                     /gene="ispE"
                     /locus_tag="ACGI59_001016"
     CDS             complement(7096..7986)
                     /gene="ispE"
                     /locus_tag="ACGI59_001016"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFG9738820.1"
                     /translation="MDFIRLKSRAKINLSIDVLGKRQDGYHFVEMIMQTIDLYDIVKI
                     KELDEDEIKVKSTSLDIPLDEDNIVYKAAKILKNKFYIKKGVEIFIEKNIPVAAGMAG
                     GSSNAAAVLVGLNHLWELRLSEDELKEIGLNLGADVPFCISGRPALAQGIGEKLTNIK
                     GLPCDTNILICKPDLFVSTKEVYQGLDLNNIKKRPNNKYLIECLKSEDIKAVSESMVN
                     ILENVTIGKHKEISDIKQVMMKNNALGSMMSGSGPTVFGLFKNKEDALIGKKELLKKY
                     KQVYVVNSSQKGVEICGEFN"
     gene            complement(8104..9654)
                     /locus_tag="ACGI59_001017"
     CDS             complement(8104..9654)
                     /locus_tag="ACGI59_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPOCS domain-containing protein"
                     /protein_id="HFG9738821.1"
                     /translation="MELIKDVIKVDNRIDFGKFQTFIEAEAVVPDKKLDVYEIVKTEG
                     YISLKKIEMADGKILCRGSFNYNVIYIADDKNTISNVDGKVDINEVIEKDNVIQDMEY
                     MLFSEVEHMDCTIMNERKIKVGALMNIRGSLFEKQRLDIVKDVAQVECIQKHRKEICF
                     QDIVGIEKAESSIRDTITINTEEIQSIISLNPCVKVKESRVTDNKVIIGGVLEINPLA
                     CTYEGELVELDRVGIEFTQFVEVPGVSDGMTEEVLLSMSDFNHIFKQNSESNTGLLEI
                     DCMACCKVKVTDEVTREVLQDAYSPQKIIKFDHKPIQLNKTLRRSEETFMVREGIRND
                     NDDIQIKDIVSVCPTMSIENSYIEGNKSIIQGIIKVEILFVPVEGLKLVYKISEEIPF
                     EHDIEMDNLTDTASVFNTACIDKVEVDLNRDQIDLIIKIKRFTEALDKKAENFIIKGE
                     DQGVYDLSKAPSIIVYICKEGDTFWNIAKKYNTTENEIAELNDIKLDEPIKPGKCLIL
                     EKKVVLVD"
     gene            complement(9748..10014)
                     /locus_tag="ACGI59_001018"
     CDS             complement(9748..10014)
                     /locus_tag="ACGI59_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HFG9738822.1"
                     /translation="MATVQTLDKIRVSLERHIGKKILLKANKGRKQIITKEGILEKVY
                     PSVFVIKLDDESNGYPRVSYSYSDLLTSNVKLQVFRDQDKLQIS"
     gene            complement(10162..11022)
                     /gene="yabG"
                     /locus_tag="ACGI59_001019"
     CDS             complement(10162..11022)
                     /gene="yabG"
                     /locus_tag="ACGI59_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation peptidase YabG"
                     /protein_id="HFG9738823.1"
                     /translation="MKVGDIVARKSHNKDIVFKIVSFGVDENNEKIAILKGIAFRVIA
                     DAYIDDLELVKAPDIKDILIDKNVENLLYKSVRKAKERQKRMTRAVPKLQLNPNTYGM
                     PGKVLQIDGDKEYLKICLDVYAQLGIPAVGVAVSEQNQYKEVRALLEKYNPDILVITG
                     HDAMTIKRGDIEDMSNYRNSSNFVKTVKEARKWQPNLDNLVIFAGACQSNYEKIISAG
                     ANYASSPGRIMIHALDPVFIVEKIACSRIDVVVPIDEVIEQTITGVKGIGGSETRGKF
                     RWAMPKTILH"
     gene            complement(11106..12044)
                     /locus_tag="ACGI59_001020"
     CDS             complement(11106..12044)
                     /locus_tag="ACGI59_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HFG9738824.1"
                     /translation="MKFLDLHCDTIAKLMENVETSELKSNKYSVDIDRLKKGDSLAQT
                     FALFVDTEEVKHPFDYCMSMANKFHEEMKKNSDEIALATNYEEIMKNQSEGKLTALLS
                     IEEGAVLEGKLENLKKFYDLGVRMMTISWNHVNELSFPHNKVEYREKGLTDFGREVVH
                     KMNELGMLVDVSHISDGGFYEIAKISSKPIIATHSNSRAMMNHSRNLTDDMIKVLANK
                     GGVTGINFFHLFLSDKSESKLEDMVRHIKHIVNVGGIDVVSLGSDFDGIDSKVEIEDI
                     SQMGKLYEPLKKEGFSEDDIEKIYYKNALRVIKEVL"
     gene            complement(12154..12315)
                     /locus_tag="ACGI59_001021"
     CDS             complement(12154..12315)
                     /locus_tag="ACGI59_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9738825.1"
                     /translation="MFILRFADDDDNLSVKDFTSLTDLKKYISENNIDKTWHQIEEVK
                     KVIPNLKEE"
     gene            complement(12325..12822)
                     /locus_tag="ACGI59_001022"
     CDS             complement(12325..12822)
                     /locus_tag="ACGI59_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HFG9738826.1"
                     /translation="MSLYTEWRNLSDNHESQEAEVKFWEEYLKVEAGIYNEILNNKMD
                     VVEGKVSELAEKFGTTNEFFMGFIDGISESLKEDIVLEEVEADKEVSIKIDWEKLYNN
                     MVTVEAHWLYNLQGWEDILPADQRKEIIKAYKKSKTIVKEAKIGRNDSCPCGSGKKYK
                     KCCGK"
     gene            complement(12937..13647)
                     /locus_tag="ACGI59_001023"
     CDS             complement(12937..13647)
                     /locus_tag="ACGI59_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0489 family protein"
                     /protein_id="HFG9738827.1"
                     /translation="MENYEYSGFYIEKPVGNNVFSYDKRENKSIYVPKLINGTLNDVR
                     LGNEVVFNEVDENKEIKAKGLENMVEYVLGNKKIYVFDNHNHAFYFWAKSLLKGEFTK
                     GCKLVHVDQHKDTREPENYDVDVNNLKDVFRYTNEVLNVGSFIKPALKYNIFSELIII
                     DSLYGFDLEVESEFVLDIDLDIFSSDMDYIPFELKFYKIKNLIKKAKVITIATSPYFI
                     NQEYAIKVLKELFNYDII"
     gene            complement(13823..14353)
                     /locus_tag="ACGI59_001024"
     CDS             complement(13823..14353)
                     /locus_tag="ACGI59_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9738828.1"
                     /translation="MDIRALPIRITKEEAIEIAKSGKNSLFSKLILKNKYAHEVRLHY
                     IEFKIITLKIHKTKSIFKNRRYSNDINELKILVNGSTGSGSIVQDFPTYEVVKNVDCN
                     IVQYSDKDERRVKAKANKMAMRLTHRFMGGIPEIEIVKIESIFRPYWVVFFGKVELNR
                     RVSYLPIEADGFVIGM"
     gene            complement(14370..14600)
                     /locus_tag="ACGI59_001025"
     CDS             complement(14370..14600)
                     /locus_tag="ACGI59_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9738829.1"
                     /translation="MSKLVSKVKKDLEIKWLGIDGFLVLLYFLITGFIFYGILTRNDI
                     IVGKIIPYGVMAVVIVIFFDYIYRVLKMTRKK"
     gene            complement(14615..16009)
                     /locus_tag="ACGI59_001026"
     CDS             complement(14615..16009)
                     /locus_tag="ACGI59_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HFG9738830.1"
                     /translation="MLTFWENGIIVIFAFILLGIGYYFSRNVKDMESYYLGNRSLPWS
                     LIVGTLVASWYGGVGTVGSVEYAAIYGISAWAVWSIAAHIGRMPLALWVGPRIHIRTN
                     ITVPDLLESFYGKHVAIIGAILMFIYCAQLGEITAMGTIGNVAWKIDKELIGLIMVGV
                     VVVLTVLGGLMGVAITDMVLFFCMVFGLTMVMPGQFEQIGGFQGLTEALKDAPQLMHP
                     TKGMSAMKALMLIVYSFGAYSDPTFYQRFSAADSPKVGRRALLTCFSLWICFDMVLTM
                     TGLIVRATYPEMAPAAGYITLVLDTLPQGIRVIFIIGILGSIISTLDSYYLAAGATLA
                     NDIYGRITKKELSQKQLVKFTRIGVCLAAVMGLSVAFRFENVYDACIFVGSIWMGSAF
                     VPIVGGLLGNGRRTSMGGILGMIVGGVTFGYFKIFPVQSFELEPLVIAIPFSFIAWMI
                     GNRIGENLNTQNNL"
     gene            complement(16135..17388)
                     /locus_tag="ACGI59_001027"
     CDS             complement(16135..17388)
                     /locus_tag="ACGI59_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HFG9738831.1"
                     /translation="MESKNNVKRFLIIFILAFGTTAMYSLPYMKSSFYDPMQQALALS
                     HTQIGNLLSLYGLVGMVSYFIGGWFADRFSVRKLITFSLIASGILGFYFSTFPSYSMI
                     LLIFVLWGFTTILTFFSASVKVVRMQGSESEQGRIFGFYEGLSGVSGTLISFIGLYFF
                     GKFAEITVGFKYVVWLYSAASIICGILLFFLVEEKKDSGASDEGLSIKSLLKVVTIPK
                     AWLIGLIIFSTYLVFSSLTYLSPYLSEVYVMPMTLVSALSIIRTYVIKMGASPVAGVI
                     TDKVGSSIRVMFVGFILMTVSTAAYLVIPKSTGFIWIAVINMIILSVILFGFRGIYFA
                     SVSESNISLETTGAVVGFASFIGFSPDAFYYTIAGNWLDKYGQTGYTYIFILSVVCAV
                     IGIFATYALNKINKRENKGLNNKIA"
     gene            complement(17439..18593)
                     /locus_tag="ACGI59_001028"
     CDS             complement(17439..18593)
                     /locus_tag="ACGI59_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFG9738832.1"
                     /translation="MSNNINQDLETKIIHWGHSADPTTGALATPICQTATFAAKTVEH
                     FEELCMTWGYVYTRECNPTLTELEAKLAMLENAESAISSTSGMGAITSTILALVKSGD
                     HIVSSDGIFSHTKLFMSELLSKFGVEVTFVDAVNPQNVKEAMRPNTKIVYIESPLNPS
                     LDLVDIKTIAEIAHENKSLAIVDSTFGTPIVQRPIDLGADLVIHSLTKFINGHGDTLG
                     GAVAGSKELIDLVRWPSLCCFTGASLPPMSAWMILRGMKTLDMRMKKHCENGLAVAEF
                     LEEEENVELVKYPALKSHPQYELCKTQMNGLGGGVVSFKLKDGISGLTRDQASRRLMN
                     SLELATIATSLGEEHTLVQMNGENLIRIAVGLESSNDIINDFKQAIEKLK"
     gene            complement(19153..19722)
                     /locus_tag="ACGI59_001029"
     CDS             complement(19153..19722)
                     /locus_tag="ACGI59_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFG9738833.1"
                     /translation="MIIIYGYCRVSTKNQTDNNSLYQQQQEILNKYNDAKIIKETYTG
                     TTTNRPKFEKIISSLKKGDLLVATKLDRLARSTSEGIQLIEGLFAKEVSVHILNIGLL
                     ENTSMGKFFLTTLLAVAELERNMIIERTQLGKEFAKKKKDFKDGRPKKYSNQQIQYAL
                     GLLNSKPYREVVKMTGISKSTLIRAKKSI"
     gene            19925..20152
                     /locus_tag="ACGI59_001030"
     CDS             19925..20152
                     /locus_tag="ACGI59_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFG9738834.1"
                     /translation="MNSLLKEAIDELKNIHCGEVFLLKELFKGYEWNRLEMNIRLKLG
                     KLFLYEVVTNPNLNVEVINKNSAYQQMYKKK"
     gene            complement(20371..21114)
                     /locus_tag="ACGI59_001031"
     CDS             complement(20371..21114)
                     /locus_tag="ACGI59_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HFG9738835.1"
                     /translation="MENYQSTNTCTYNILYVQFNEDFERSKYFSYDVKYPVIYNIRNQ
                     STYVNPVILNNINNAVRMTVNNFKNGLQEEEREYNNAASQNSLPKQSYWVSTSYAVTF
                     NKNYVLSMILNLMGFANNYGPKYNTLNNYNIDLTTGKELLLNDIFRDGVDYINIITDY
                     IKSQINENREMYYSNVELVIPEDQAFYLTDNGIIIYFGVDEIAPESFGIPKFELKFSD
                     FGNFINLRFYCISPDIYVQSRGRAKHFMG"
     gene            21282..22154
                     /locus_tag="ACGI59_001032"
     CDS             21282..22154
                     /locus_tag="ACGI59_001032"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFG9738836.1"
                     /translation="MLLLADKWKDYELIDMGNGEKLERWGSYVLRRPDPQVVWPMESE
                     WALWKNPHGHYHRSNKGGGQWEHKKRYPEQWTINYKNLKFHIKPTGFKHTGLFPEQAA
                     NWDWMIEKIKKANRPIKVLNLFAYTGGATVACASAGAEVCHVDAAKGMVNWAKENIEL
                     SGLKNQTVRFIVDDVVKFVEREIRRGKKYDAIIMDPPSYGRGPKGEVWQIEEKLYSFV
                     DLCMGVLSDNPLFFLINSYTTGFSPIVLENVLSTTIGKKTNTGDIYGGELGIPASRDG
                     KILPCGIFGRWEKK"
     gene            22154..22819
                     /locus_tag="ACGI59_001033"
     CDS             22154..22819
                     /locus_tag="ACGI59_001033"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFG9738837.1"
                     /translation="MIKVIYEDNHLLVVEKPVNILSQGDNTNDKDMVNLLKDYIKKKY
                     NKPGNVYIGLIHRLDRPVGGVMVFAKTSKAASRLSEQVRSKTFKKTYLAVVHGKIKSN
                     SDILKDFLYKNKKTNMVTVVKKSHKDAKEAELNYNVLDFKENLSLVQVNLKTGRSHQI
                     RVQFASRKHPLYGDQRYGKEINKIGQQISLWSNKIEICHPTTKEKMEFTCHPPDKYPW
                     NIF"
     gene            22840..23697
                     /locus_tag="ACGI59_001034"
     CDS             22840..23697
                     /locus_tag="ACGI59_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFG9738838.1"
                     /translation="MSKKFSIGEMSKLHKISIQTLRYYDQIDLFKPVHVDQESNYRYY
                     SIDQFSQLDIIKYLKYLGMPLKEIKSKLDENNEEILKLLTNKINIVDEKIKELELIKK
                     VLSTKKETMEKGMKVDEVGKIVRKHIPRRSILSIEYEEGLSSEETMELSRRQIANILE
                     DNISVFYGGVSGLVSIDKFLKEDKVIYDNSFVTVERDLFDIKSQKHITEISSGDFICM
                     TYKGPYKENYTAFKKLVDYVEYKRIPIENYIYEIPIIDPLSTNNEEELLTEIQIQVKK
                     REIDLRCLV"
     gene            complement(23853..26399)
                     /locus_tag="ACGI59_001035"
     CDS             complement(23853..26399)
                     /locus_tag="ACGI59_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9738839.1"
                     /translation="MNIFNKVALQGLKKNRTRTLVTIIGVVLSAAMITAVTTFGVSLM
                     NYMINGAIIKYGDWHVQFLDVDSSFVQEQRVNDEVANTATFENIGYATLEGNRNPDKP
                     YLFIAGFSKEAFDTLPITLISGRLPENSREILVSSKVATDGGVKFAVGDTISLDVGKR
                     MSENEKLGQSDPYTAGSEVFVPQDKKTYTVVGICRKPVFEESSAPGYTLITKEDKQVK
                     TDSLSLFVTLKNPHQIRSYTSSTKDHASIFNDNVLRFIGVSDNNLFNMFLYSVGGIVL
                     AIIMLGSIFLIYNSFNMSLNERTHQFGILSSVGATAKQLRNSVLFEGICIGAIGIPIG
                     VAIGIVSIGLVISVISKNFANILYVNVPLTLTLSIPAIIVAAVVSMVTILISAYIPAR
                     KAANTPVMECIRQTNEVKIESKSVKTSKLTQRVYGLEGVLAMKNFKRNKKRYRSIVLS
                     LVLSIVLFISTSALVADLKQMSNQRSEVTDQDIIFSTQGMKDNEMLKLYDKLKTANGV
                     YEGLYQKLMTYSCVAKVSELTDDYLNYEGSHSMSEMLELPVDMQFLDDRTYLNIIKSL
                     GLPAEEYTGQNAKLIAVSMVTKDNNIIDIFNSSSMNVSLTPETNGKLEIPERQNVNIS
                     FVKVVPPDSPPGIRSSDVKKASFRVLAPYSAKDKFEIPGVSTGIESLSFQSKNPTQSV
                     AEMEQIIESEGVTNEYNLYNMYGMMDENRNYIFIANVFAYTFIIMISLISIANVFNTI
                     STNIKLRRRELAMLRSVGMSEHDFQKMMNFECAFYGMRALFFGLPIAVISSWLIHKVM
                     VTDDIDFVIPWFSIGISIFSVLFIVFITMLYTINKIKKENIIDALRDDMA"
     gene            complement(26399..27085)
                     /locus_tag="ACGI59_001036"
     CDS             complement(26399..27085)
                     /locus_tag="ACGI59_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9738840.1"
                     /translation="MAFLKIEDLCKVYGKNENKVTALDHVSLTIEKGEFTAIIGSSGS
                     GKSTLLHSIAGVDVPTSGKIYLEGQDVYGQSNEKLAIFRRRQVGLIYQFHNLIPTLNV
                     VENITLPILMDKRKVNQERLNDLLELLGLKERKTHLPNQLSGGQQQRVAIGRALMNAP
                     AVMLADEPTGSLDSKNGQEIIQLLKESHSKYHQTLIIVTHDENIALQADRIICISDGK
                     VVRDERKVNR"
     gene            complement(27168..28157)
                     /locus_tag="ACGI59_001037"
     CDS             complement(27168..28157)
                     /locus_tag="ACGI59_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFG9738841.1"
                     /translation="MFRNREFRKFAILFLLITILTVALGFAISIMTGILSIVSATTFG
                     IAFFVFTKNRYKSIAQISEQINLVLHNANHLYIAESDEGELSILQSEITKMTLRIREQ
                     NYALKKEKEHLADSLADIAHQLRTPLTSVTLILSLLENTSDEDERKELIRETEELLIR
                     MDWLITSLLKLSRLDAGIVVFQKEQIDVNNLISSALHQLLIPMELHNITLHIDIPKGV
                     RILGDLNWLSEAIQNIFKNCMESVGDNGKIDIICEDNFLFTQLTIHDNGAGFKKEDLP
                     CLFNRFYRGKNSSTAGYGIGLALCKTIIMRQGGTITAQNHPQGGAIFVIRFPK"
     gene            complement(28144..28830)
                     /locus_tag="ACGI59_001038"
     CDS             complement(28144..28830)
                     /locus_tag="ACGI59_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFG9738842.1"
                     /translation="MKRIFLVEDDKAIAKNLILLLNSEGFIVTHVSTRSEALVEIAKN
                     KFDLALIDISLPDGNGFTICTEIKEKQDIPVIFLTASGDESSVVTGLNMGADDYITKP
                     FRPRELIARIRTALRKSGRFGSTFEICGLYVDTATGVVKKNGNEVFLSALEYRLLLVF
                     ISNPKSIITRVRLLDELWDAAGEFVNDNTLTVYIKRLREKIEDDPSSPQIILTVRGTG
                     YRLGGSYVSK"
     gene            complement(28827..28949)
                     /locus_tag="ACGI59_001039"
     CDS             complement(28827..28949)
                     /locus_tag="ACGI59_001039"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9738843.1"
                     /translation="MDYIRTKETTENCVMISKIVVYYCSAGIYKTNKIKEDESK"
     gene            complement(29330..32536)
                     /gene="carB"
                     /locus_tag="ACGI59_001040"
     CDS             complement(29330..32536)
                     /gene="carB"
                     /locus_tag="ACGI59_001040"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFG9738844.1"
                     /translation="MPKLDSIKKTLVLGSGPIIIGQAAEFDYSGTQACQALKEEGIEV
                     VLVNSNPATIMTDKEIADKIYIEPLTIEFIEKIIEKERPDSLLAGMGGQTGLNLAVEL
                     HDAGILDKYNVKVIGTSIESIKKGEDRDLFREVMKEINQPVIVSDIVTNLEAGLEFAN
                     KIGYPVVVRPAYTLGGTGGGIADTEEELREILSHGLQLSPVGQVLLEKSIKGWKEIEY
                     EVMRDGNGNCITVCNMENVDPVGVHTGDSIVVAPSQTLSDEEYQLLRKASIDIINAIE
                     VQGGCNVQIALNPHSLEYAIIEINPRVSRSSALASKATGYPIAKVAAKIALGYTLDEI
                     ENAVTKKTYACFEPTLDYVVVKIPKWPFDKFRHANRKLGTKMMATGEIMSIGSNFEAA
                     ILKGIRSLETGKYSLVHAPSEGRTLEELKARVVVPDDERLFDLAEMIRRGYKVEMISE
                     ITGVDKWFINKFKWIVEQEEKLKVLKIEDLDKEYLHELKKKGFSDKGISDLMKISPEK
                     LYELRSLYNIQPVYKMVDTCGGEFEALSPYYYSTYEQYDEVVVSDKRKVVVLGSGPIR
                     IGQGIEFDYCSVHCVKSLRKMGIETIIVNNNPETVSTDFDTSDKLYFEPLTEEEVLNI
                     IEKEKPEGVILQFGGQTAIKLAKFLHEKNIPILGTDFRDIDAAEDREKFDDLLERLDI
                     NRPKGKGVWTTNEGVEIAKELGYPVLVRPSYVLGGQGMEITYNEEKLIQYLDDAFDRD
                     HKNPVLIDKYLTGREIEVDAICDKEDILIPGIMEHLERAGVHSGDSTTMYPSQNISDE
                     IKEKIVEYTKKIALDLNVLGMVNIQFIEFQNELYIIEVNPRASRTVPYISKVSGVPIV
                     DLATKCMLGAKLKDLGYGTGVYKEPKLVSVKVPVFSMSKLSKVEVSLGPEMKSTGEVL
                     GVGENLEEALYKGFLAAGRHMSDERGVVLATVNNHDKDEFIEIAKDMKELGYTFVATE
                     GTAKSLRENGIEADIVNRIEEPRPNILDAIRNKQVDIVINTPTKGNDSTRDGFKMRRT
                     AIEFSTEIMTSLDTLKALVEVKKKHLNKDKLKVYNIAE"
     gene            complement(32729..33778)
                     /gene="carA"
                     /locus_tag="ACGI59_001041"
     CDS             complement(32729..33778)
                     /gene="carA"
                     /locus_tag="ACGI59_001041"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002845470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HFG9738845.1"
                     /translation="MKARLILEDGTVFIGKAFGYLEESVGEVVFNTSMIGYGEVLTDP
                     SYYGQIVTMTYPLVGNYGINLSSAESEKVQVKGFIVREKSDSPSNFRCEIDIDQYLKQ
                     NKVIGLEGIDTRALTKILRNNGTMKGIITLEDSKLEDVKHKLDKFSNTEAVRTVTRKE
                     VEHIKGNGPKVAVMDFGVKRNILRSFIARGCDITIFPATTSPEDVLSINPDLIFLSNG
                     PGDPEDLEDVIENIKALIGKKPIVGICLGHQLLALALGGKTAKLKFGHRGGNHPVKDL
                     EEGKVFITSQNHGYYVSEVPEQMKVTHINLNDNTVEGMRHEKLDVYSVQYHPEACPGP
                     KDNDYIFDKFLELVK"
     gene            complement(34035..37241)
                     /gene="carB"
                     /locus_tag="ACGI59_001042"
     CDS             complement(34035..37241)
                     /gene="carB"
                     /locus_tag="ACGI59_001042"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFG9738846.1"
                     /translation="MPKLDSIKKTLVLGSGPIIIGQAAEFDYSGTQACQSLKEEGIEV
                     VLINSNPATIMTDKEVADKIYIEPLTIEFIEKIIEKERPDSLLAGMGGQTGLNLAVEL
                     YEKGILDKYGVKIIGTSVESIKKGEDRDIFREVMKEINQPVIVSDIVTDLQAGLDYAL
                     TIGYPVVVRPAYTLGGTGGGIADNEEELREILSHGLQLSPVGQVLIEKSIKGWKEIEY
                     EVIRDSKGNCIVVCNMENIDPVGVHTGDSIVVAPTQTLSNKECAMLKKASLDILNAVE
                     VQGGCNVQFALNPHSFEYAVIEINPRVSRSSALASKATGYPIAKVSSKIALGYTLDEI
                     ENAVTKKTYACFEPTIDYVVAKIPKWPFDKFKKANRKLGTKMMATGEIMSIGSNFEAA
                     ILKGIRSLETGKYSLVHTPSEERSIEELKKRVVVPDDERLFDLAEMIRRGYKVEMIEQ
                     ITGIDKWFINKFKWIVEQEEKLKVMKIEDLTKDYLLELKKKGFSDKGISDLMKISPEK
                     LYELRSLYNIKPAYKMVDTCAGEIDAISPYYYSTYEQYDEVVVSDKKKVIVLGSGPIR
                     IGQGIEFDYCSVHCVKSLRKMDIETIIINNNPETVSTDFDTSDKLYFEPLTEEEVLSI
                     IEKEKPEGVILQFGGQTAIKLAKFLHEKNIPILGTDFDDIDAAEDREKFDDLLERLDI
                     NRPKGKGVWSLNEGIEVANELGYPVLVRPSYVLGGQGMEITYNEEKLSQYLQDAFDRD
                     HKNPVLIDKYLTGREIEVDAICDKEDILIPGIMEHLERAGVHSGDSTTMYPSQNISDE
                     IKEKIVEYTKKIALDLNVLGMVNIQFIEFQNELYIIEVNPRASRTVPYISKVSGVPIV
                     DLATKCMLGAKLKDLGYGTGVYKEPKLVSVKVPVFSMSKLSKVEVSLGPEMKSTGEVL
                     GVGENLEEALYKGFLAAGRHMSDERGVVLATVNNHDKDEFIEIAKDMKELGYTFVATE
                     GTANSLRENGIEADIVNRVEESRPNILDAIRNKQVDIVINTPTKGNDSTRDGFKIRRT
                     AIEFSTEIMTSLDTLKALVEVKKKHLNKDELKVYNIAE"
     gene            complement(37294..38337)
                     /gene="carA"
                     /locus_tag="ACGI59_001043"
     CDS             complement(37294..38337)
                     /gene="carA"
                     /locus_tag="ACGI59_001043"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HFG9738847.1"
                     /translation="MKGKLILEDGTVFEGKAFGYLKEAIGEVVFNTSMAGYGELLTDA
                     TYYGQIVTMTYPLIGNYGINLEDVESNKVQVKGLIVREKSDNPNNFRCEMDIDTYLKQ
                     NKVIGLEGIDTRALTKIIRNNGTKRGIITLENVSLQDVQSKLEEHCNKDSVKNVTRKE
                     IENIKGTGAKVVVIDFGVKNSVLESLKACNCDITIVPAMTTVEEILNINPDLILLSNG
                     PGNPEDINDIVENIKKLIGKKPLTGIGLGHQILALALGGKTSKLVFGHRGGNHPVKCL
                     ETGKVFITSQNHGYNVSEMPENTEVTHINLNDNTIEGMRHKELPIYSVQYIPEYTTKE
                     DLDCIFNKFLALI"
     gene            complement(38363..39079)
                     /gene="pyrF"
                     /locus_tag="ACGI59_001044"
     CDS             complement(38363..39079)
                     /gene="pyrF"
                     /locus_tag="ACGI59_001044"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFG9738848.1"
                     /translation="MINGKEKLIVAIDTDEFDKAKELIDRLEDSVDIFKVGLEQYVAT
                     KGKTIDYLKEKGKKIFLDLKFHDIPNTMKSAVKAAVRDNVWLMTIHVSDMEGMRQCAL
                     IAREEAERLNIEKPLVVGVTVLTSLSNQDLQDIGCNMTTEELAIKRAKLAKESGIDGV
                     VCSAQEVDKIVEACGTDFITVCPGIRPKSAEVGDQKRVVTPSDAIKKGAHYLVVGRPI
                     TKAENPRESAINIVREIENA"
     gene            complement(39455..40090)
                     /locus_tag="ACGI59_001045"
     CDS             complement(39455..40090)
                     /locus_tag="ACGI59_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1847 domain-containing protein"
                     /protein_id="HFG9738849.1"
                     /translation="MYKCGKCNLRGCSNGNMDKLMKICPTREIELQQKAKQLYEEEEN
                     RKIAYNAGLVEAEGYCQYTRLKEIIEFAKKCGYKKIGIAFCIGLKREMQTVQKIFEHH
                     GFEVFSVICKNGAMPKSTIGLEASQTISKCSDEVMCNPIGQALFLNEAKVDLNVILGL
                     CVGHDTLAMKYMEAPITTLVVKDRVTGHNPVAAIYNAESYFEKKLFDKDEE"
     gene            complement(40114..41028)
                     /locus_tag="ACGI59_001046"
     CDS             complement(40114..41028)
                     /locus_tag="ACGI59_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFG9738850.1"
                     /translation="MELYEAIFYRKSVRNFSSKKIKKPLMEEIKRSCKNLEYLNEDLN
                     IKAHVVDRGHIIHFLMGKECKVKAPHYIVVTSDKGEDYLQNIGFATEGVVLRLTTLGI
                     ATCWLEGNLKREDILEFVDIGNKNFEEDEEDDLDYLAGIKTEEDDENKIENPYIIIAF
                     GYPAKNEELFRTRYARPDRKPVKDMINKINRNRKWKDILELTRRAPSVKNSQPWYFHK
                     DERGLHLFEKRPKKHCEDMNKVSLGVALRHFDIACIKNKIDVSYEKLPIRNKIGKSYF
                     ITVVEHVKPEEETQEENVTLEKEESQDE"
BASE COUNT        12983 a         7722 c         4626 g        16092 t
ORIGIN      
        1 actttcagtt ttagtattta atccacatca gataataatc ttaaaatgac caaggtctta
       61 aaactattta tctttatcag atttatcttc taaattttta tttatatcaa taacattgtt
      121 tctttcttct tcatcttttt ctaattttat agttaattgt tcttcaactt ctttagaaga
      181 attttcctct gtaactgcat tttcaatttc attcagaggt aactctttat taaatgcttt
      241 tacaaattgt tctgcatcta aagtttcata tataagtaaa gcttgtgcta catactctaa
      301 tctatccata ttttcttgta atatacttct tgtcttatca tatgcaccat caactatacg
      361 ctttgtttct tggtctatct caaaagcaac ttcttcagaa taatttctct tacttgaaaa
      421 actattacct aggaatactt catcatcact gtcaaatgac attggaccaa gtttagaact
      481 cattccatac tttgttacca tacttcttgc agtagctgta actctttcta agtcatttga
      541 agcacctgta gatacatcct taagtactag ctcttctgct actctaccac caagtaaaac
      601 tataatattt tctttcattt cattttttgt agcatagaac ttatcttcaa caggaagttg
      661 cattgtaaac cctccagctc ttcctcttgg aactattgta acttgatgaa caggacttac
      721 ttcttcaagt acatgggcac aaactgcatg ccctccttca tggtatgctg taagtcttct
      781 ttctttctca cttattactt tcgatttttt agccacacct gctataacct ttgttatagc
      841 ttcttctata gtttccatct taattttctt ttctcttttt ctagctgtta atattgcagc
      901 ctcattcatt aagttctcta tatcagcagg agtaaatcct ggtgttcttc tagccaagac
      961 atccattttt acatcatcac ttaatggttt atttcttgag tgaactttaa atatagcctc
     1021 tctacctttt acatctggtg taccaacaac aacttgtctg tcaaatcttc ctggtctaag
     1081 taaagctggg tcaagtatat ctggtctatt tgtagctgcc atgattatta taccttgatt
     1141 taccccaaat ccatccattt caactagaag ttgattaaga gtttgctctc tttcatcatg
     1201 acctccacca agacctgcac ctctttttct accaactgca tcaatttcat ctatgaatat
     1261 gatagctgga gcacttttct tagcctgttc aaataaatct cttactcttg aagcaccaac
     1321 cccaacaaac atctcaacga aatcagaacc acttatactg aaaaagggaa ctcctgcttc
     1381 tcctgcaact gctcttgata gatatgtttt accagttcca ggaggtccta ccataagcat
     1441 tccttttggt attcttgcac ccaattcaat atatttctta ggattcttta agaaatctac
     1501 tacctcctgc aaatcttctt tttcctcatc tagtccagct acatctttaa atgtaactct
     1561 agttttttca tcatctttat gaaccttagc ttttgatttg ccaaaactca taacctttcc
     1621 gcctccacct tgagactgat tcataaacac gaaccaaata atcaccatga agaatattaa
     1681 cagtaatgta ggcaacattt cgacaaacca aggtgtagat ggcttagctt caccaccaaa
     1741 agttagtttc ccagccttag cttggtctaa cacctcatct gcaagtttat ttcccatcac
     1801 ttctgttgga acatatgact taaactttgt atttgtatct tttatagtac cttctacaga
     1861 agtttgattt acaaagtata atcttgatat attctcatct gttaattctc tatatacttc
     1921 agagaatttt aaatctttta ttttttctgt aggcttacct gagaattgaa ctatacctac
     1981 tataattata aaaacaagta gataaaaacc tgcccctttt agcaacttat tcaatataga
     2041 agccctcctt tcatacattg ctttgcccta taatttgtta aaattaattt ttaatacttc
     2101 ttttgtgctt tcatcaattt tataattttc actgatttta taatctccaa cacatattat
     2161 tccttcacca tctgtgatta taggaatttt acatctttct tctcttggga tttttaaatc
     2221 tatgaataaa tctttcactt ttttacttcc catagcaagc tttatcttat caccttgcct
     2281 tctgcttctt attactatac ccccttttac cttattaaaa tcaaaccctt tagatgaatt
     2341 gtctaatttc atactcttat acctatctat gctcattact tgggtctcaa taatttgttt
     2401 taattctttt attttaataa acccattact aggtacattg taataaaatt caatttcctc
     2461 attaacaatt tcctcattcg ttaatattat actgtctttt tttctataaa caaatatatt
     2521 tcttggaaga gttaacattt tatttacttt tgaattatct tctaaagaca ttatatcttc
     2581 tatatgtctt tggtcaacaa aattagtatc tcctagtata tgttttatgg aatttctaag
     2641 aactctaact ttgatagcat catgtaagca aacaaaatcg tctaaattta tctctacaaa
     2701 gcctttttct tttatatttg aaacttctct aaatttagct tctgcctctt tctcaatata
     2761 atcgttatca ctttttaaac tattgctcat cctcactata gattctatta catttgaatt
     2821 aaaattatcc ttcatatatg gtaaaaggtc taaccttatc ttatttctag tgtatatatt
     2881 ttctaaattt gttttatcta tcctcggatt taaattataa gcttcacaat actcctctat
     2941 ctcatttctt tccacgtcta atatgggtct aattatgcaa ttatctctaa tataatcaat
     3001 tccctttaag ccttttaaac ctgttcctct catgatacgc atcataaccg tttcggcttg
     3061 gtcattgaga ttatgaccta ttgctatttt attggcttta atcttatcct taatttcata
     3121 aaacatttca tatcttagct tccttgctcc ttcttctaat gataatccct cattttcaca
     3181 atattttggt acattaatag actttacaaa gaatataatt cccatatcct cacaaagttt
     3241 agatacatat aatgcatctt tttgagcttc tatccctctt atttgatggt ttaaatgtgc
     3301 tgcatatatt tctatattaa aatctttttt taatctattt agtacatgca ataaacaaac
     3361 tgaatcaggt cctccagaaa gacctaaaac tattttatcg cctttttgta ttaaattatg
     3421 tttatttata gtacttagta ccttatcaaa tatcatattt atccctctta cacttagttt
     3481 tattcacttt taagtatact ttctataaaa aaacgctaaa cccttgtatc aattttaaga
     3541 aattaaagtt taatataaat ataataatac caaataaatt aaataaaagg tagttttttc
     3601 actaccttta taaaatttct ggaaaatagc catagttatt atttagtacc tatgtatata
     3661 tctattttat tcctacctaa aaataaatct tgtaccttta tattatagtc tattttcaat
     3721 tttatgtgtt ccccttcttg tatatttata tctaattctc ttgtatcaat atcaattaat
     3781 aaagtacctt gtggggtcac atactttgta gtactacttt ctccacattt aaacagcatt
     3841 gttgagttaa tagcaccaaa ctttttgata ctaacttgat ttttaagtat ctttatagta
     3901 ttagttattt ctatatcttc ttctttttcc ttgtaaataa catatattcc atcattttta
     3961 taaaaaattt taccaaaggc agtcatctct atggtgtcca tatttccttt ttcatcatat
     4021 tgtctagtat ttatacttat ttttacactc aacgattttt cctccataga ttcttcatat
     4081 aaactagtat ttttgtattt ctactttttg aatcttatca atttttttct ttaagaactc
     4141 tcttgctata tctgaaaatt tttcaggtat atctgataca taatattgat aagttccatg
     4201 aatttctgta ttgtttataa tattttgcac ctctaaaatc ttttttaaat cctttgcagt
     4261 ctctttagct ggattaacaa gttttatatg ctctccaact tcttcaccta ttgttctctt
     4321 taaaatagga taatgagtac atcctaacac taaagaatct atattttttt ctttcaattc
     4381 ttgtaaatat atctttgctg tcagtttagc cacttctgta tttgcccatc cttcttcaac
     4441 aataggcaca aataaaggac atgctttatt aacaatttct atactctcat ctattttaga
     4501 tatttccaag ttgtatgctt ttgaacttat agttccttca gttcctatta ttcctacaat
     4561 tttgtttttt gtagaactta cagctgttct agctcctgct tctataactc caattatcgg
     4621 tatatcatat ttttcttgag cttcttttaa gcttcttgca gtagcagtgt tacatgctat
     4681 aactatagct tttacacctt ttgtttttaa aaagtttata gcttgaaaag tatatttaat
     4741 tatagtttct ttggatcttg aaccatatgg tattctggca gtatctccaa agtatattat
     4801 atcctcattt ggtaatatct tcattatctc ttttagaact gttaatccac ctaatcccga
     4861 atcaaaaact cctattggtt tattgttcat ctttatacaa cacctacttt acatttttgt
     4921 gtttaacaac aacttattta tcgctaaaat ttatatttat attcataatt aatatattat
     4981 tacctaaatg attattatag tttattttac acaaaaaata aaggagctat cttaaattag
     5041 aaaataattt ctacaaaaag acaactcctt tcactactta aaaatttaaa tttattttgc
     5101 aaacacatga tttcctattc tcatataagg attcaatcta tttatccatt tgcaagtggc
     5161 tatttctgga ttccaaaaat atactgcttt attagttggg tcagttccat aaagagcttc
     5221 ctgagctgct ttacgtgctg agtctgttgg tgccatcttt attgaaccat caagcacaac
     5281 tgagaaagca tttttttgat atatgacttc ttctattgtg tctggaaatc tagggtccaa
     5341 tactctattt attactacag ctgctacagc aacctgtccc tcatagcttt ctcctctagc
     5401 ttctcctgca accaacttag ataataacat caagtcatta tcagataaat ttattacttc
     5461 tttctttgct atattttttt cttgtttgtt tactttctta atattttctg ttggttcata
     5521 tgcaaatacc tctttaatag gcataaatat aaaagtcaaa gctaatagtg tagccatcag
     5581 tttactccac ctgtccatga ttaaaaccac tccttacatt cttattttta aaattttttc
     5641 tctaaatcct tgactacttc aagtacttta aacttaattt ttactctact tgtttttttt
     5701 ggtgtatctt gttttattaa cttatattct ttatcagtta aaacttcctt caacttatca
     5761 tctgtagatt tatctatcac tccattagat tcatcaacaa aacatagtgg agggaacata
     5821 acacaccacc agttttttcc ttcacctttc ccaataataa tctttaatgc tttatattca
     5881 ccagctggaa gaactatatt agaatattgt ttagtaggga aattgctata ttgtatacct
     5941 acctttacac tgtaattata tccattttta agaataatat ttttacttat ttcttccaga
     6001 ctactatatt ccttttttat aatagcttca ctttctttta tactttttga attttgaagc
     6061 tttggctgta aatattttat tacttcatct ctaactttta gtttaagttc ttggtcttca
     6121 tctgtattgc tgtttgctat aacatgaaat cttattaatt tatctttata atcttttgat
     6181 atattgtcta ctttttttac ctcaccattt attactattg tcattattga tatgactgat
     6241 ataagactta atattaatag tcccaatcta atcttaattt ttctcattga aaattccccc
     6301 tcaagataaa tttatatcat caattatgtc caattaaatt taatttattc ataatatttt
     6361 ttaatactaa taaaaaaagc tatcttaaaa tgattacaat cattttaaaa tagcttttta
     6421 aaccttatat tttataagtc tttactaatc atagccttat ctatcataaa ctgctctgct
     6481 ttttctatat gctctgtagc atacttcttg gatagctcac tatcaccttt aattatagca
     6541 tcaagtaaat atttatgctc ttcaacaata ttatgtgatg tatcataagc agacatatag
     6601 gtaactctaa atctatatac ttgttcccac aatgagttta ttaattggat taatttttta
     6661 ttattagttg ctttaaatat acattcatga aattctacat caagttttaa taacgcatct
     6721 acatcggatt ccaaagtgct ttttttaaat tcttctgata tatctttaag ctttttaata
     6781 tcttcatcac taattctttc agcggcaaga tatgctgcta agccttctag gcttgctctt
     6841 acttctaata catccataat atcctttaat gacatatttg ccacatacgc accttttcta
     6901 ggcaacataa cgactaaacc ttctaattca agctttctta ttgcctctct cactggagtc
     6961 ctacttactc ctaattgttc tgctagttgc acttccatta gcctttgacc aggctttaat
     7021 tttccttcta agattgcttc tcttaaattc tcaaagacta catctcttaa tggcttgtaa
     7081 ttatccaagt tcagtttagt taaattctcc acaaatttca accccttttt gactgctatt
     7141 aacaacatac acctgtttat atttttttaa taattctttt tttccaatta gagcatcttc
     7201 tttattttta aatagtccaa aaactgtagg accacttcca ctcatcatag aacctaaagc
     7261 attatttttc atcattactt gttttatatc acttatttct ttgtgtttac ctatagtaac
     7321 attttcaaga atatttacca tgctttctga tacagctttt atatcttcag attttaaaca
     7381 ctctatcaaa tatttattat ttggtctttt tttaatatta ttcaaatcta atccttgata
     7441 aacttcttta gtagaaacga ataagtctgg tttgcatatc aatatatttg tatcacatgg
     7501 taaacctttg atatttgtta gcttttctcc aataccttga gctaaggcag gtcttcctga
     7561 tatacaaaaa ggaacatctg cgcccaaatt aagtcctatt tcttttaatt catcttcaga
     7621 taatctaagt tcccataggt gatttaaacc tactaatact gcggctgcat tagaacttcc
     7681 tccagccatc cctgctgcta caggaatatt tttttctatg aatatttcta cacctttttt
     7741 tatataaaat ttatttttaa gtattttggc agctttataa acaatattat cttcatctaa
     7801 aggaatatct aaactagtgc tttttacctt tatttcatct tcgtctaatt cttttatctt
     7861 tactatatcg tataaatcta tcgtttgcat tatcatttca acaaaatgat aaccgtcttg
     7921 cctttttccc aagacatcaa tggataaatt tatttttgct ctactcttta gtcttataaa
     7981 atccattcta tcctccttgt ttaaagaata atgcatcact aacaataata ttatactaca
     8041 aaaaataaaa taatagggta ttataatacc ctattatact taattacttt aaatttttta
     8101 atattaatct actaatacga cttttttttc taaaataaga cacttcccag gcttaattgg
     8161 ttcatcaagt tttatatcat taagttctgc aatttcattt tctgttgtat tgtatttttt
     8221 tgctatattc cagaaagtat ctccttcttt gcatatatat acgattatgc taggagcttt
     8281 agataaatca tatacacctt ggtcttctcc cttgataata aaattttctg ctttcttatc
     8341 taatgcttca gtaaatctct taattttgat aatcaagtct atctggtccc tattcaaatc
     8401 aacttcgact ttatcaatac atgctgtatt aaacacagat gctgtatcag ttagattatc
     8461 catttctata tcatgttcaa atggaatttc ctcactaatt ttataaacca attttaaacc
     8521 ttcaactggg acaaataaaa tttcaacttt tatgattcct tgtataatgc ttttatttcc
     8581 ttcaatataa ctattttcta tagacatggt tggacaaacg cttacaatat cttttatttg
     8641 tatatcatca ttatcatttc ttatgccttc tctaaccata aatgtttctt cactacgtct
     8701 taaagtctta tttaattgaa taggtttgtg gtcaaatttt attatctttt gaggcgaata
     8761 tgcatcttgt aatacttctc ttgtaacttc atctgtaact tttaccttac aacaagccat
     8821 acaatctatt tctaaaagac ctgtgttgct ttcactattt tgcttgaaaa tatgattaaa
     8881 atctgacata gacaacaata cttcttcagt cataccatca ctaactccag ggacttctac
     8941 aaattgagta aactcaatac ctactctatc caactctaca agttcaccct catatgtaca
     9001 agctaacgga tttatttcta atactccacc aataataact ttattgtcag tcactctact
     9061 ttcttttact ttaacacatg gatttaaact tattatagat tgaatttcct ctgtgtttat
     9121 tgttatagta tctcttatag aactttctgc tttttctatt ccaactatat cttgaaaaca
     9181 aatttccttt ctgtgctttt gtatgcattc cacttgcgcc acatctttta caatgtctaa
     9241 cctttgcttt tcaaacaaac ttccccttat attcataaga gctcctactt taatctttct
     9301 ctcattcatt atagtacaat ccatatgctc tacttctgaa aataacatgt attccatatc
     9361 ttgaataaca ttgtcttttt ctattacttc gtttatatct acttttccgt ctacattaga
     9421 aatagtattt ttatcatctg caatatagat tacattataa ttaaaactac ctctgcaaag
     9481 tatcttccca tctgccattt caattttttt aagtgatatg tatccttctg tttttacaat
     9541 ttcatataca tctaattttt tgtctggcac aactgcctcc gcttcaatga atgtttgaaa
     9601 ttttccaaag tctattctat tgtcaacttt aattacatct ttaattaatt ccatagtcta
     9661 cctccttaag tattataaaa atatcacact atatttatat ttaaggagca aaaaaaaaat
     9721 tacacctatt tttaaatagg tataatttta acttatttgt aatttatctt gatctctaaa
     9781 aacttgcaac tttacattag atgttaataa atctgagtaa ctataagata ctctaggata
     9841 tccattactt tcatcatcta actttataac aaacacgctt ggataaactt tttctagtat
     9901 accctctttt gtaataatct gctttcttcc tttattagct ttcaacaata ttttctttcc
     9961 tatatgtctc tctaagctta ctcttatctt atctagagtt tgaacagtag ccacaatatc
    10021 accttcttta acgtttatat gtttacataa tatcacatat aaacattttt gtcaaatgtt
    10081 taaactaatt attttatata ttttttgcaa aaatgtcaat actatttttg aagatataca
    10141 gtataattat gttaattttt gctaatgtaa tattgttttt ggcattgccc atctaaattt
    10201 tcctcttgtt tcagaaccac caattccctt aacacctgta atagtctgct ctataacctc
    10261 atcaatagga actactacat caattcttga acaagcaatt ttctccacta taaaaacagg
    10321 gtctaatgca tgtatcatta ttcttccagg cgaacttgca tagtttgctc ctgcacttat
    10381 tattttttca taatttgatt ggcatgctcc tgcgaatatg acaagattat ccaaatttgg
    10441 ttgccattta cgtgcttctt ttactgtttt aacaaaattt gaagagtttc tatagttact
    10501 catatcttct atatctcctc tttttattgt cattgcatca tgacctgtta ttactagtat
    10561 atccggatta tatttttcta gtagtgctct tacttcttta tactgatttt gctctgaaac
    10621 agctacacct actgcaggta ttccaagttg agcatataca tccaagcaaa tttttaaata
    10681 ttctttatct ccatctatct gtagtacttt tccaggcatt ccatatgtgt taggatttaa
    10741 ttgaagtttt ggaactgctc tagtcattct tttttgtctt tctttagctt ttcttactga
    10801 cttatataat aaattttcta catttttatc tattaatata tcttttatat caggtgcttt
    10861 tactaattct aggtcatcaa tataagcatc tgctattact ctaaatgcaa ttccctttag
    10921 tatagctatt ttctcattat tttcatctac accaaaagag actattttaa atactatatc
    10981 tttgttatgt gactttctag ctacaatgtc tcctaccttc atagttaaac ctccaataaa
    11041 tttttatctt agtttatact atgttcgata taaaactttg tttcctaatg tcaaaaaaac
    11101 atttcttata atacttcttt tatcactctt aaagcatttt tatagtatat cttttctata
    11161 tcatcttcac taaacccttc tttttttaga ggttcataca gtttacccat ctgagatata
    11221 tcttcaatct caacttttga atctattcca tcaaaatctg accctagaga tacaacatct
    11281 attcctccaa catttacaat atgttttatg tgtctaacca tatcttcaag tttactttca
    11341 cttttatcac ttaaaaataa gtggaagaag tttatacctg taactccacc tttatttgct
    11401 aatactttaa tcatatcatc agttaaattt ctagaatgat tcatcattgc tcttgaatta
    11461 gaatgtgtag caattattgg tttagaagaa atctttgcaa tttcataaaa tccaccatct
    11521 gaaatatgag aaacatcaac caacattccc aattcattca tcttatgtac cacttctctt
    11581 ccaaaatcag taagcccttt ttctctatat tctactttat tatgaggaaa gctcaattca
    11641 ttcacatgat tccaacttat tgtcatcatt ctaaccccta agtcgtaaaa tttctttaaa
    11701 ttttctaatt taccttcaag tactgcacct tcctcaatag ataaaagagc tgtaagttta
    11761 ccttctgatt ggtttttcat aatttcttca tagttagtag ctaaagctat ttcatcacta
    11821 ttttttttca tttcctcatg aaatttattt gccatactca tgcaataatc aaaaggatgt
    11881 tttacttctt cagtatcaac aaaaagagca aatgtttgag ctaatgaatc cccctttttc
    11941 aatctgtcta tatcaacaga atatttatta gacttaagtt cacttgtttc tacattttcc
    12001 attaattttg ctatagtatc acaatgtaaa tcaagaaatt tcatatttat accccctgta
    12061 tattttatat ttatatctaa aaatagaaat agttgtcctt atagacaact attgacaact
    12121 attttttaat attcttttta atattactta atcttattct tcttttaagt taggtattac
    12181 ttttttaact tcttcaattt gatgccatgt tttatctata ttattttcac taatatattt
    12241 ttttaaatca gttaaagatg taaaatcttt aactgataaa ttgtcatcat catctgcaaa
    12301 tcttaaaata aacatgagac tcccttactt tccacaacat ttcttatatt tttttccgct
    12361 tccacatggg catgaatcat ttcttcctat ttttgcttct ttaactattg ttttagactt
    12421 cttataagcc tttattattt cttttctttg gtctgctggt agtatatctt cccaaccttg
    12481 taaattatat agccaatgag cctcaacagt taccatattg ttatataatt tttcccaatc
    12541 tatttttata gaaacttctt tatcagcttc tacttcttct aaaactatat cttcttttaa
    12601 gctttcactt attccatcta tgaatcccat gaaaaattca ttagtagttc caaatttttc
    12661 agccaattca cttactttac cttcaacaac atccatttta ttatttaata tttcattgta
    12721 tattccagct tcaactttca aatattcttc ccagaatttt acttctgctt cttgactttc
    12781 atgattatca cttaaatttc tccattcagt atataaactc atactttttc ctcctaagta
    12841 tacctatatt tttatagttt ttatagtata aaatctttta gttagattgg aagtttcttc
    12901 tttttgatta taccatattc tatttaaagt taactcttat attatatcat aattaaacaa
    12961 ttcttttaat acttttatag catactcttg gtttataaaa taagggcttg tcgcaattgt
    13021 tattactttg gcttttttaa ttaagttttt tatcttgtaa aatttgagtt caaatggtat
    13081 atagtccata tcacttgaaa atatatccaa atctatatca agaacaaact ctgattctac
    13141 ttctaaatca aatccatata gactatctat tattattaat tctgaaaata tgttatattt
    13201 taaagcaggt tttataaaac ttcccacatt taaaacttca tttgtatatc taaatacatc
    13261 ttttagatta tttacatcta catcatagtt ctcaggttct ctagtatcct tatgttggtc
    13321 tacatgcact aacttgcatc cttttgtaaa ctcacccttt aacaaagatt ttgcccaaaa
    13381 ataaaatgca tggttatgat tgtcaaacac atatatcttt ttatttccca ggacatattc
    13441 gaccatattc tccaatcctt ttgcttttat ttccttattt tcatctactt cattaaatac
    13501 tacctcattg cctaacctta catcatttaa tgtaccattt attagttttg gaacgtatat
    13561 gctcttattt tctcttttat catatgaaaa tacattattt cctactggtt tttctatata
    13621 aaatccacta tattcataat tttccataaa actttatttc ctccattgtt atataaatat
    13681 ataagttata attttaaaaa ttaaaatgca cttattgtaa atcatatcat ctattataga
    13741 aaacatctta tataactaaa tataatatct tctctcaata tgtattatac aataagtgca
    13801 aaatatattt atataaaata tactacattc ctataacaaa accatcagct tcaattggaa
    13861 ggtaactaac tcttctattc aactccacct ttccaaaaaa tactacccaa tatggtctaa
    13921 aaatactttc aattttaact atttcaatct ctgggattcc tcccataaac ctatgagtta
    13981 atctcatagc cattttattt gcctttgctt ttactcttcg ttcgtcctta tcactatact
    14041 gcacaatatt acaatccaca ttttttacaa cttcatatgt aggaaaatct tgtactatac
    14101 taccagaacc tgtagaacca ttcactaaaa tttttaattc atttatatca ttagaatatc
    14161 tcctattctt gaaaatggat ttcgttttgt gaattttaag tgtgataatt ttaaactcta
    14221 tataatgaag tctaacttca tgagcatatt tgttctttaa tattagctta ctaaataaag
    14281 aattttttcc acttttagct atctctatag cttcttcttt tgtaattctt ataggtaacg
    14341 ctcttatatc cataaaatca ttctctttct tatttttttc ttgtcatttt taaaactcta
    14401 tatatataat caaaaaatat tacaattaca actgccataa ctccatatgg aattattttt
    14461 ccaactataa tatcatttct agtcaatata ccataaaata taaatcctgt tattaaaaag
    14521 tacaataata ctagaaaacc gtctattcca agccatttta tttctaaatc tttttttacc
    14581 ttagacacta atttagacat agcagtttct ccttttatag attattttgt gtatttaaat
    14641 tttcaccgat tctattacca atcatccatg caataaatga aaaaggtata gctatcacaa
    14701 gaggctctaa ctcaaagctc tgaactggaa atattttaaa ataaccaaaa gtcactccac
    14761 caacaatcat tcctaaaata ccacccatag atgttcttct tccatttcca agaagcccac
    14821 ctactatagg tacaaatgcc gaacccatcc aaatacttcc tacaaatata catgcatcat
    14881 atacattttc aaatctaaat gctacgctaa gccccataac tgctgctaaa catacaccta
    14941 ttctagtaaa ctttactaat tgtttctgag ataactcttt ttttgtaatt cttccatata
    15001 tatcatttgc taatgtagcc ccagctgcta gataatagct atccaatgtt gatattatag
    15061 aacctaaaat tcctattata aatattactc ttattccttg tggtaatgta tctaaaacta
    15121 aagttatata tccagcagct ggagccattt ctggataagt agctcttact ataagtcctg
    15181 tcatagttaa caccatgtca aaacatatcc atagtgaaaa acaggttaat aaggctcttc
    15241 ttccaacctt aggagaatct gctgctgaaa atctttgata aaaagttggg tctgaataag
    15301 ctccaaaaga atatacaatt aacatcaatg ctttcatagc tgacatacct tttgtagggt
    15361 gcataagctg tggtgcatct ttgagtgctt ctgtaagacc ttgaaaccct cctatttgtt
    15421 caaattgacc tggcataacc atagttaaac caaataccat acaaaaaaac aataccatat
    15481 ctgtaatggc aactcccata agcccaccaa gtactgtaag tacaacgact actcctacca
    15541 ttattaatcc tattaattct ttatctattt tccatgctac gttacctata gttcccatag
    15601 cagttatttc tccaagttgt gcacaatata taaacatcaa aattgcccca attatagcaa
    15661 catgcttgcc ataaaaactt tccagtaaat caggaactgt tatattggtt cttatatgaa
    15721 tccttggacc aacccacaat gctaaaggca ttctcccaat atgtgcagct attgaccaaa
    15781 ctgcccatgc agatattcca tatatagccg catactctac actacctact gtaccaactc
    15841 ctccatacca tgatgcaacc aaagttccta ctattaagga ccatggtaaa cttcgatttc
    15901 ctaggtaata actttccata tcttttacat ttctagaaaa ataataacct attcctaata
    15961 atataaatgc aaatataact ataatcccat tttcccaaaa tgttaacata tctaatctcc
    16021 acattcttat attttaattt ttaactttaa tatctaaata tgaacaactc tacccatttc
    16081 cataaaaata ggtagagtta tataataatt ataagtattt aagtaatctt gttattaagc
    16141 aattttattg tttaaacctt tattttctct tttatttatt ttatttaaag cataagtagc
    16201 aaatatccct ataactgcac atactacaga taaaataaat atatatgtat atcctgtttg
    16261 tccatattta tctaaccaat tacctgcaat agtgtaataa aacgcatctg gagaaaatcc
    16321 tataaatgaa gcaaatccaa ctactgctcc tgtagtttct aaagatatat tagactctga
    16381 aacagatgca aagtatatac ctctaaatcc aaataaaatt acacttagta ttatcatgtt
    16441 tattacagct atccatataa atcctgtact cttaggaata accaagtaag cagctgtact
    16501 tactgtcatt aatataaatc ctacaaacat tactcttata gatgaaccaa ctttatctgt
    16561 tataacacca gcaactggag atgctcccat ttttataaca taagttctta ttattgataa
    16621 agcagaaact agagtcattg gcattacata aacttctgat aagtatggac ttaaataagt
    16681 taaacttgag aatactaaat aagttgaaaa tattataaga ccaattaacc aagcctttgg
    16741 tattgtaact acttttaaca atgacttaat acttaatcct tcatctgatg ctccactatc
    16801 ttttttctct tccactaaga aaaatagaag tataccacat attatagatg ctgcagaata
    16861 taaccatact acatatttga atcctactgt tatttcagca aatttaccaa agaaataaag
    16921 acctataaaa gaaattaatg tacctgatac acctgataac ccttcataaa aaccaaatat
    16981 tcttccttgc tctgactcac ttccttgcat tctaacaacc ttaacagagg ctgagaagaa
    17041 ggttaatatt gtcgtaaatc cccataatac aaatattaaa agtatcatac tatatgatgg
    17101 gaatgtagag aaataaaatc caagtatccc tgaagcaatt aaagaaaaag ttattaattt
    17161 tctaactgag aatctatctg caaaccatcc tcctataaaa taagatacca ttcctactaa
    17221 tccatataaa ctcaataagt tccctatttg tgtatgactt aatgctaaag cttgttgcat
    17281 tgggtcataa aatgatgatt tcatatatgg taaactatac atagctgttg ttccaaatgc
    17341 caatatgaat attatcaaaa atcttttaac attatttttg ctttccattg attctctctc
    17401 cttaaaattt tattgtttat aatctcattc atataatttt attttaattt ttctattgct
    17461 tgtttaaaat catttattat atcatttgaa gattctaatc ctacagctat tcttattaga
    17521 ttttccccat tcatttgaac taatgtatgc tcttccccta agcttgttgc tattgttgca
    17581 agctctaaag agttcattaa tcttctacta gcttggtctc ttgtcaatcc actgatacca
    17641 tccttaagtt taaatgaaac tacgcctcca cctaaaccat tcatttgagt tttacaaagc
    17701 tcatattgag gatgtgattt taatgctgga tactttacca attccacatt ctcttcttcc
    17761 tctaaaaact ctgctactgc taatccattt tcacaatgct ttttcatcct catatctaac
    17821 gttttcatac cccttaaaat catccatgca ctcattggtg gtaaagatgc tcctgtaaaa
    17881 caacataaac ttggccatct aactaaatct attaattctt ttgaacctgc tactgctccc
    17941 cctaaagtat ctccatgtcc atttataaac ttagtaagac tatgtattac taaatctgca
    18001 cctaaatcta ttggtctttg tacaattggt gtcccaaaag tactatcaac tatagctaag
    18061 cttttatttt catgagcaat ttctgctatt gtttttatat ctactaagtc taatgatgga
    18121 tttaaaggac tttctatata aactatttta gtatttggtc tcattgcttc ttttacattt
    18181 tgtggattta ctgcatctac aaatgtaact tcaactccaa atttagataa caactcagac
    18241 atgaataatt tagtgtgtga aaatattcca tctgaactaa ctatatggtc accacttttt
    18301 accaatgcta atatagtaga tgttattgcc cccatgcctg atgtactaga tatggcactt
    18361 tctgcatttt caagcatagc caacttagct tctaactctg taagagtagg attacactct
    18421 cttgtataca catatcccca tgtcatacac aactcttcaa aatgctctac tgtctttgca
    18481 gcaaatgttg ctgtttgaca tattggtgta gccaatgcac ctgttgttgg gtctgcacta
    18541 tgtccccaat gtattatttt tgtctctaaa tcttgattaa tattgttact cattttgatt
    18601 tcctcccatt ttgacttatt tatacaacgt tttccctgcc aaatttcatc aatttagctt
    18661 agtcttaata cattttttca agtatacttt tttatttgtt ataatttcta aaaatatgac
    18721 acttattttg tttttttaca gttctaatta taaactctat agtaactata gagtcaatat
    18781 gtttttgata ttttttttac aatgtttaaa atagtcaata caaaaatagg tatatcttag
    18841 aataactaaa gtcatcctaa gatataccta tttttatatc acaatataaa taattacata
    18901 taagcattag tttttttatc ataaatagat ttttcaagat ttatcatttt ttcataaata
    18961 tattatagtt aaatatttta atattaccta acatctacaa taaaatatac tcttaaaata
    19021 aaattatata attaaaaata ctctataaaa gctaaaaagt ttttctattc gctacatcta
    19081 aaaataatta gatagaggta taaatgtatt tagtttattt atacctctag tcttttataa
    19141 attatatctc tactaaattg attttttagc acgaataagt gtgctcttgg agataccagt
    19201 catcttcaca acctctctat atggcttaga atttaataac cccaatgcat attgtatctg
    19261 ttgattgctg tatttctttg gcctaccatc tttaaagtcc tttttctttt tagcaaattc
    19321 ttttcctaat tgagtacgtt ctattatcat atttctctca agttctgcaa ctgcaagtaa
    19381 tgtagtcaaa aagaatttac ccattgaagt attttctaaa aggcctatat ttaaaatatg
    19441 aacacttact tctttagcaa ataaaccttc tattagttgt attccttctg atgtacttct
    19501 agctagtcta tccagcttag ttgcaacaag taagtctccc ttttttagag atgatataat
    19561 tttttcaaat ttaggcctgt ttgtagtagt gcctgtataa gtttccttta ttattttagc
    19621 atcattatat ttattcaaaa tttcttgctg ttgctgatac aaactgttgt tgtctgtttg
    19681 attttttgta cttacacggc aatatccata tataataatc atcacttcct aacataaaga
    19741 ttttgtacag aagttaccca gaagcttgtt tttacttatc tttatccatt ttactaaaac
    19801 gatttttggg tgtcaatact gttaactttt gacaccataa taattccttt ataatttgtt
    19861 tgactatgtc aaaaattacc aatataatga aattaatgag tttatacaaa ttggaggaaa
    19921 taatatgaat agtctgttaa aagaagcaat agatgaatta aaaaacatcc attgcgggga
    19981 agttttttta ttaaaagaat tgttcaaagg ctatgaatgg aacaggttgg agatgaatat
    20041 cagattgaaa ttaggaaaac tttttttgta tgaagtggta actaatccca atttaaatgt
    20101 agaggtcatc aataaaaatt ctgcttatca gcaaatgtat aaaaaaaaat agtgacttaa
    20161 atgtatataa tagtataatc ttcttattga ttcaaaagtt aaaactttta atacaaaaca
    20221 atcattctat tttaacattt atcaatatgt ctaaatttat ataattaaca catacgaata
    20281 gtattgttta aatcacatta tttaagatag ataaatctta accctactcc agcataaata
    20341 ctaaaatagg gcttagaatt atctatattt ttatcccata aaatgcttag ctcttcctct
    20401 tgattgaaca tatatatctg gggaaataca atagaagcgt aaatttataa aatttccaaa
    20461 gtcagaaaac ttcagttcaa acttaggtat accaaaactt tcaggtgcta tttcgtcaac
    20521 gccaaaatag atgataattc cattatctgt aagataaaat gcttggtctt caggtataac
    20581 taactcaaca ttagaataat acatttctct attttcattt atttgagatt taatgtaatc
    20641 agtaattata ttaatataat caactccatc tctaaaaatg tcattaagta aaagttcttt
    20701 tccagttgtc aaatctatat tgtaattatt caatgtattg tatttaggcc catagttatt
    20761 agcaaacccc ataaggttta atatcatact cagtacataa tttttattaa aagtaactgc
    20821 gtaactggta gatacccaat agctttgttt tggaagtgaa ttttgagaag ctgcgttatt
    20881 atattctcgt tcttcttcct gtaagccatt tttaaaatta tttacagtca ttcttacagc
    20941 attgtttatg ttatttaaaa taactgggtt tacatacgtg gattgatttc taatattgta
    21001 tataacagga tactttacat catatgaaaa atacttactt ctttcaaaat cttcattaaa
    21061 ttgtacatat aatatattat atgtacaggt atttgtagat tgataatttt ccattaaaat
    21121 cctccccctt ttataaaaaa atcagataca atataatatg cttaaaacag taataatgtt
    21181 aatggtaaat gtaaagaata gaaagtatct aaaacagcaa aaaatgacta attttgtggt
    21241 ataatacata ggattgagag ttattttata ggaggaaaaa tatgttatta ttagcagata
    21301 aatggaaaga ttatgaactt atagatatgg gaaatggaga aaaattagaa cgttggggaa
    21361 gttatgtact tagaagacca gacccacagg ttgtatggcc aatggaatct gaatgggctt
    21421 tatggaaaaa ccctcatgga cattatcaca gaagcaataa aggtggaggt caatgggaac
    21481 acaaaaaaag atatccagaa caatggacta taaattataa aaacttaaag tttcacatta
    21541 aaccaactgg ctttaaacat actggattat tcccagagca agcagctaac tgggattgga
    21601 tgatagagaa aataaagaaa gctaacagac ctataaaagt gttgaatctt tttgcatata
    21661 caggtggagc tactgtagca tgtgcatctg ctggtgcaga agtatgtcat gttgatgctg
    21721 caaaaggtat ggttaattgg gcaaaagaaa atatagaatt atcaggtctt aaaaatcaaa
    21781 cagtaagatt tatagtggat gatgttgtaa agtttgttga aagagaaata agaagaggta
    21841 aaaaatatga tgcaataata atggaccctc cttcttatgg aagaggtcct aaaggagaag
    21901 tatggcaaat agaagaaaag ttatacagtt ttgtagattt atgtatggga gttttatcag
    21961 ataacccttt atttttctta attaattctt atacaacagg attttctcca atagttcttg
    22021 aaaatgtatt gtctacaaca ataggtaaaa agacaaatac aggagatata tacggtgggg
    22081 aattaggaat accagcttcc agagatggta agatacttcc ttgtggtata tttggaagat
    22141 gggagaaaaa ataatgataa aagtcatata tgaagataac catttattag ttgtagaaaa
    22201 accagttaac atattgtctc aaggagataa tactaatgat aaagatatgg taaatctttt
    22261 aaaagactat attaaaaaaa agtataacaa acctggaaat gtatatattg gtttaataca
    22321 tagattggat agaccagtag gtggagttat ggtgtttgca aaaacatcaa aagcagcatc
    22381 tagattgtct gagcaggtaa gaagtaagac tttcaaaaaa acttatctag ctgttgttca
    22441 tggcaagata aaatctaact cagatatact aaaagatttt ttatataaaa ataaaaaaac
    22501 taatatggta actgtagtga aaaaaagcca taaagatgcc aaagaagctg aacttaacta
    22561 taatgtatta gattttaaag aaaatctaag tttagttcaa gttaatttaa agactggtcg
    22621 ttctcatcaa ataagagttc aatttgcaag cagaaaacat ccattatacg gagaccaaag
    22681 atatggcaaa gaaataaata agattggaca acaaatatct ctttggtcca ataagataga
    22741 aatttgccat cctactacaa aagaaaaaat ggaatttaca tgtcatccac ctgacaaata
    22801 tccatggaat atattttaaa tctcaaatga ggtgattata tgagtaaaaa attttcaata
    22861 ggagaaatgt ctaaacttca taaaataagc atacagacac taaggtacta tgaccaaata
    22921 gatttattta aacctgttca tgttgaccaa gaaagcaatt acagatatta ctcaattgac
    22981 caattttcac aattagatat tataaaatat ttaaaatatt taggtatgcc tttaaaagaa
    23041 attaagtcaa agcttgatga aaataatgaa gaaattctaa aattactaac aaataaaatt
    23101 aatatagtgg atgaaaaaat taaagaatta gagcttataa agaaggtact aagcacaaag
    23161 aaagaaacta tggaaaaagg tatgaaagta gatgaagttg gtaaaatagt gagaaaacat
    23221 attccacgaa gaagtatctt atctatagag tatgaagaag gattaagtag tgaagaaact
    23281 atggaacttt ccagaagaca aatagcaaat atattagaag ataatatatc tgtgttctat
    23341 ggtggagtaa gtggacttgt atctatagat aaatttttaa aagaagataa agttatttat
    23401 gacaatagtt ttgttactgt tgaaagagac ttatttgata taaaatcaca aaaacatata
    23461 acagaaattt catctggtga ttttatttgt atgacatata aagggcctta caaagaaaat
    23521 tataccgctt ttaagaaact ggttgattat gttgaatata aaagaatacc tattgaaaat
    23581 tatatatatg aaatacctat aatagaccct ctgtctacaa acaacgaaga ggaacttcta
    23641 acagaaattc aaatacaagt taaaaagaga gaaattgact taagatgtct agtgtagtaa
    23701 tctagtttac ttacacatct taatatagtt gtctattatc aacatatgat ttttctagat
    23761 agtgattcct aacagtgaga ttttcaaaaa ataggaggaa atatggctaa tgctaattcc
    23821 ctcctatttt taattaagcc atatcatttt aattaagcca tgtcatcacg aagtgcatca
    23881 ataatatttt ctttttttat cttattaata gtatacaaca ttgtaataaa tactataaat
    23941 aaaacactaa atatactaat tccaatacta aaccatggaa tcacaaaatc aatatcatct
    24001 gtaaccatta ctttatgaat tagccatgaa gaaattactg ctataggaag tccaaagaac
    24061 aatgctctca tgccataaaa ggcacactca aaattcatca tcttttggaa atcatgctca
    24121 gacattccta cagagcgaag catggcaagt tctctacgac gtaacttaat attagttgaa
    24181 attgtattaa acacattggc aatcgaaatc agagaaatca taattatgaa agtataagca
    24241 aaaacattgg caataaaaat gtaattacgg ttctcatcca tcattccata catattgtac
    24301 agattgtact cattcgtaac tccctcactc tcaataattt gctccatttc tgcaactgac
    24361 tgagtaggat ttttcgattg aaagcttaaa ctttcaattc ctgtagaaac accaggaatc
    24421 tcaaacttat ctttggctga ataaggagcc aatactctga agcttgcttt cttcacatct
    24481 gaacttctga ttcctggagg agaatcaggt ggcactactt tgacaaatga aatattcaca
    24541 ttttgtctct caggtatctc caacttgcca ttagtttcag gagtgaggct aacattcata
    24601 gaagaactgt tgaaaatatc aataatatta ttatcctttg ttaccataga aacagcaatc
    24661 agttttgcat tttgtccagt atattcttct gctggtaatc caagactttt aataatattc
    24721 agataagttc tatcatcaag aaattgcata tctactggta attcaagcat ttcactcata
    24781 gaatgtgaac cctcatagtt caaataatcg tctgtaagct cacttacttt ggcaacacat
    24841 gaataggtca taagtttctg atacaaacct tcgtaaacac catttgcagt ttttagctta
    24901 tcgtaaagct tcaacatttc gttgtctttc ataccttgtg tagaaaaaat aatatcctgg
    24961 tcagtaacct ctgacctctg atttgacatc tgttttaggt ctgctactaa agcacttgta
    25021 gatataaata gaactatgct taaaacaagc gacaatacaa tacttcgata acgtttcttg
    25081 tttcttttaa agtttttcat agcaagaact ccctcaagac cataaactcg ctgtgttagt
    25141 tttgatgtct tcacagattt agattcaatt tttacctcat ttgtctgacg aatacactcc
    25201 atcactggtg tgttggcagc ctttcttgct ggtatgtaag cagaaattaa gattgtaacc
    25261 atacttacca ctgctgcaac gatgattgca gggatagaca atgttaatgt caacggaacg
    25321 tttacataga gaatatttgc aaagttttta gatatgacag aaatcacaag tccaatactg
    25381 actataccga ttgcaacacc aattggtata ccaatcgcac caatgcaaat tccttcaaat
    25441 agcaccgaat ttcgtaactg ttttgctgta gctccaacag atgaaagtat tccaaattga
    25501 tgtgttcgct cgttcaatga catgttaaat gaattatata ttagaaaaat tgagcctagc
    25561 atgattatgg caagcacaat gccaccaacc gagtacaaaa acatattgaa caaattgtta
    25621 tcagagacac ctatgaatcg caacacattg tcattgaaaa tggaagcatg gtctttagtg
    25681 ctacttgtgt aagagcgaat ctgatgtgga tttttcaatg tgacaaaaag gctaagactg
    25741 tcagtcttga cttgtttatc ttctttggtt atcaaggtat atcctggtgc agaagattct
    25801 tcaaatacgg gctttctaca gatacccaca actgtataag ttttcttatc ctgtggcaca
    25861 aaaacttcac tcccagcagt gtatgggtca ctctgaccaa gcttttcatt ttcactcatt
    25921 cgtttcccta catccaaaga aatagtatcc cctactgcaa atttgacacc accatctgtc
    25981 gcaactttac ttgaaacaag aatctctcta ctattttcag gtaatcttcc agaaattaaa
    26041 gttataggca aagtatcaaa agcttctttg ctaaatccag caataaaaag atatggttta
    26101 tctggatttc tgttaccttc aagtgttgca tagccaatat tttcaaatgt tgcagtattt
    26161 gcaacttcat cattgactct ttgttcctgt acaaaagaag aatccacatc caaaaactgg
    26221 acatgccaat caccatattt tataatagct ccatttatca tatagttcat caaagaaact
    26281 ccaaaggtag taactgctgt aatcatagca gctgacagaa caactcctat aattgttaca
    26341 agagttcgag ttcgattctt tttcaatcct tgcagagcaa ctttgttaaa aatgttcatt
    26401 aacgattcac cttcctctca tcacgcacta cttttccatc tgaaatacag ataattctat
    26461 ctgcctgtaa agcaatgttt tcatcatgtg tgacaataat gagagtttgg tgatatttac
    26521 tatggctctc tttcagtaac tggataatct cttgtccatt tttactgtct aagctacctg
    26581 taggctcatc agcaagcata actgctggtg cattcatcaa tgcacgacca atggcaacac
    26641 gttgctgttg gccaccagaa agttgatttg gtaaatgtgt ttttcgttct tttaaaccaa
    26701 gtaattctaa caagtcattt aaccgttctt gattgacctt acgcttatcc ataagtatag
    26761 gcaatgtgat attttccact acatttaatg tgggtatcag attgtgaaac tgataaatca
    26821 atccaacttg tcgcctacga aaaatagcaa gtttttcatt gctttggcca tatacatctt
    26881 gcccctccag atatatcttt ccacttgttg gtacgtctac tcctgcaatg gaatgtagta
    26941 gtgtagattt accagaacca gaagaaccaa tgattgcagt aaactcccct ttttcaatag
    27001 taagtgagac atggtcaagt gcagtaacct tattttcatt cttaccataa accttacata
    27061 aatcttcaat ctttaaaaac gccattatat aagtctcctt tcatatttta taaatatatc
    27121 atagaacttt caacttacat ttaagtgaca tttaaatgag agattcgtca ctttggaaaa
    27181 cgaataacaa atattgcacc accttgtggg tgattttgtg cagtaattgt tcctccttgt
    27241 ctcataataa tcgtcttaca aagtgccaat cctattccat aaccggctgt gcttgaattt
    27301 ttcccacgat aaaatctgtt aaatagacat ggtaaatctt cttttttaaa gcctgctccg
    27361 ttgtcatgaa tagtaagttg agtaaacaaa aagttgtcct cacaaatgat atcaattttt
    27421 ccattatcac ccacactctc catgcagttt ttaaatatat tttgaattgc ttctgaaagc
    27481 caatttaaat cacccaaaat ccttactcct tttggtatat caatatgtaa agtgatattg
    27541 tgcagttcca tgggaatcaa aagttgatga agtgcagaac taatcaaatt attcacatca
    27601 atttgttctt tttgaaagac tacaatacct gcatctaaac gtgataattt taaaagggaa
    27661 gtaatcagcc agtccattcg tataagtaat tcctctgttt ctcgtatcaa ctcttttctc
    27721 tcatcttcat cagaagtatt ctctaacagt gacaaaataa gagttactga tgtaagagga
    27781 gtgcgaagct ggtgggctat gtcagctagt gagtcagcaa ggtgttcttt ttcttttttc
    27841 agtgcgtaat tttgctctct aatgcgtaac gtcattttag ttatttcact ttgtagaata
    27901 gaaagctcac cctcatctga ttcagcaata tacagatggt tagcattatg aagcacaaga
    27961 ttaatttgtt ctgaaatctg tgcaatactt ttgtatcgat ttttagtaaa cacaaaaaat
    28021 gctattccaa aggtagtggc agaaacaata gaaagtattc cagtcataat actgattgca
    28081 aatcccaggg ctacagtgag aatagtaatt aataagaata aaatagcaaa ttttcgaaac
    28141 tctctatttc gaaacataac ttccccccaa tctataccct gtcccacgaa cagtcaaaat
    28201 tatttgtgga cttgaagggt catcttctat cttctctcgc aagcgtttga tgtacacagt
    28261 caatgtgttg tcattaacaa actcacctgc cgcatcccac aattcatcaa gtaatcttac
    28321 cctcgtaata atacttttag gattgctaat aaacaccaat aacaaacggt attctaaagc
    28381 tgaaagaaaa acctcattgc cattcttttt cacaacacca gttgctgtat ctacataaag
    28441 cccacaaatt tcaaaagttg acccaaaacg cccacttttt cgtagtgctg ttctaattcg
    28501 cgcaatcagt tcacgtggac gaaaaggttt agtaatataa tcatctgcac ccatattcag
    28561 tccagtaaca acacttgatt catcaccgga agctgtcaaa aagataacag gaatgtcttg
    28621 cttttctttg atttctgtgc aaatcgtaaa cccatttcca tcaggtaaag aaatatcaat
    28681 aagtgccaaa tcaaatttat tcttagcaat ctctacaagt gcttcacttc ttgtggatac
    28741 atgggttact ataaatcctt ctgaattaag caaaagaata agatttttgg caattgcttt
    28801 atcatcctca actaaaaata tccgtttcat ttactttcat cctcctttat tttattagtt
    28861 ttatatattc cagcagagca atagtacact actattttac ttatcattac acaattttca
    28921 gtagtttctt ttgttcttat ataatccatg cataatacct ccattgattt attatatttc
    28981 ttataacata aatacacaag ataagacaaa aaacataatg taagtgcaag ttttttaact
    29041 cactaactta taaacataca ctcctactaa cactactttc ttgatgacac atcttacaag
    29101 cttatattca tacatcaatg aaaaattaaa tttatccatt tattaaaata aaaggcaata
    29161 gcttctatag ctaatgccct ttatcttaaa atgatttaaa tactatacaa tttttataaa
    29221 tggctataaa taaactcgta tagaatataa aatttatcta cagtcttttg ttatacaaaa
    29281 tttataacct cttatagaac aaatttacca ctttgcaaac ttatcacttt tactcagcta
    29341 tattataaac ttttaattta tctttgttta aatgtttctt cttaacttct actaaagctt
    29401 ttagcgtatc caaagatgtc attatttcag tagaaaattc aatggctgtt cttctcatct
    29461 taaatccatc tcttgtagag tcatttcctt tagttggtgt atttattact atatcaactt
    29521 gtttatttct tatagcatct aatatgtttg gtctaggttc ttcaattcta tttactatat
    29581 cagcctctat tccattttct cttaaactct tcgctgttcc ttctgtagca acaaaagtat
    29641 atcctaattc tttcatatct tttgctatct ctataaactc atctttatca tgattattta
    29701 cagtagcaag tactactcct ctttcatcag acatatgtct acctgctgcc aagaaccctt
    29761 tatataaggc ttcttctaaa ttttctccta cacctaggac ttctcctgta gacttcattt
    29821 caggtcctag acttacttca actttagata atttagacat tgagaatact ggtactttta
    29881 ctgatactaa ttttggctct ttgtaaactc ctgtaccata tcctaaatcc tttaatttag
    29941 ctcctaacat acacttagta gccaagtcaa ctattggtac tccacttact ttacttatat
    30001 atggaactgt tctactagct ctagggttaa cctcaattat atataattca ttttgaaatt
    30061 ctataaactg tatattaacc ataccaagca cattaaggtc taaagctatt tttttagtgt
    30121 attctactat cttttctttt atttcatcag atatattttg acttggatac attgttgtac
    30181 tatctccaga gtgaactcca gctctttcta aatgctccat tataccaggt atcaatatat
    30241 cttctttatc acatatagca tctacttcta tttctctacc agttagatat ttatctataa
    30301 gtactggatt tttatggtct ctatcaaatg catcatccaa atactgaatt agtttttctt
    30361 cgttataagt tatttccata ccttgaccac caagtacata agatggtcta actaatactg
    30421 gataacctaa ctccttagca atttcaacac cttcattagt tgtccataca ccttttcctt
    30481 ttggtctatt tatatctagt ctctctagta aatcatcaaa tttttctcta tcttctgctg
    30541 catctatatc tctaaagtct gttcccagta taggtatatt tttttcatgt aaaaacttag
    30601 ccaattttat agctgtttgt ccaccaaact gtagtattac accttctggt ttttcttttt
    30661 ctattatatt taaaacttct tcttctgtta atggttcaaa atacaactta tcagatgtat
    30721 caaaatccgt acttacagtt tcagggttat tgtttacaat tatagtttct attcccattt
    30781 ttcttaaaga ttttacacag tgaactgaac aatagtcaaa ttctattcct tgacctattc
    30841 ttattggacc agaacccaat actacaactt ttctcttatc actaactact acctcatcat
    30901 attgttcata agttgaatag taataaggtg atagagcttc aaattctcct ccacatgtat
    30961 caaccatttt ataaactggt tgtatattat ataaacttct caattcatat aatttttctg
    31021 ggcttatctt cattaagtca gatatacctt tatcagaaaa tccctttttc tttaattcat
    31081 gtaaatattc tttatctaag tcttctattt ttagaacttt taacttttct tcttgttcta
    31141 ctatccattt aaatttattt ataaaccact tatcaacacc agttatttct gatatcattt
    31201 ctactttata gcctcttctt atcatttcag ctaagtcaaa tagtctctca tcatcaggta
    31261 ctactactct agcctttaat tcttctaatg ttctaccttc tgatggagca tgaaccaatg
    31321 aatattttcc tgtttcaagt gatcttatac ctttaagaat agcagcctca aagttacttc
    31381 ctatactcat gatttcacca gttgccatca tcttagttcc taattttcta ttggcatgtc
    31441 taaatttatc aaatggccac tttggtattt ttactaccac ataatctaat gttggctcaa
    31501 aacaagcata agttttttta gttactgcat tttctatctc atccaaagta taacctaaag
    31561 ctatcttagc tgctactttt gctattgggt atcctgtagc ttttgatgct agggctgatg
    31621 acctactaac tcttgggttt atttctatta tcgcatattc taaactatgt gggtttaaag
    31681 ctatttgaac gttacatcca ccttgaacct ctattgcatt tattatgtct attgatgcct
    31741 ttctaagtag ttgatactct tcatcactta gagtttgact tggagcaaca actatactgt
    31801 ctcccgtatg aactccaact gggtctacat tttccatatt acatacagtt atacagtttc
    31861 catttccatc tctcattact tcatattcta tttctttcca accttttata ctcttttcaa
    31921 gaagaacttg tccaacagga cttagctgta gtccatgtga aagtatttct cttaattctt
    31981 cctcagtatc agctatacct ccaccagttc cacctagcgt atatgctggt cttacaacta
    32041 ctggatatcc tatcttattg gcaaactcaa gacctgcttc taaatttgta actatatcac
    32101 taactattac tggttgattt atttctttca ttacttctct aaataaatct ctatcttcgc
    32161 ccttttttat agattctata gatgttccta taacttttac attatattta tctaatatac
    32221 cagcatcatg taattctaca gctaagttta atcctgtctg accacccata cctgcaagta
    32281 aactgtctgg tctttctttt tctattattt tttctataaa ttctattgtt aatggttcta
    32341 tatatatttt atctgcaatt tctttatcag tcattatagt agctgggtta ctatttacta
    32401 atacaacttc aattccctct tcttttaatg cttggcaggc ctgtgttcct gaatagtcaa
    32461 actctgcagc ttgacctatt attattggcc ctgaccctaa tactaaagtt ttctttatac
    32521 tatctaattt aggcataact ctttctcctt atctaaacaa ttttcacagt tcttatcttc
    32581 atcatctata caatcaactt taattacttt attttatctt aagttcaaat ttactcatca
    32641 atacatctat ttaatacatc tatgttaatt aaaccaaact ttttgattta agttattctt
    32701 gttttttagt tagctattct aatgttgttt attttactaa ttctaaaaat ttatcaaaaa
    32761 tatagtcatt atcttttggt cctgggcaag cttcaggatg gtattgaaca ctgtatacat
    32821 ctagcttctc atgtcgcatt ccttcaacag tattatcatt taaatttata tgtgtcactt
    32881 tcatttgttc aggaacttct gaaacataat aaccatgatt ttgagaagtt ataaatactt
    32941 ttccttcttc taaatcttta actggatgat ttccacctct atgcccaaat ttaagtttag
    33001 cagtttttcc tccaagagct aaagctaaaa gttgatgtcc taagcatatt ccaactatag
    33061 gttttttacc aataagagct tttatatttt ctataacatc ttctaagtct tctgggtctc
    33121 caggtccatt tgacaagaat attaagtcag ggtttatact caatacatct tctggagatg
    33181 ttgtagctgg aaatattgtt atatcacatc cacgagctat aaatgatctt aatatatttc
    33241 tttttactcc aaaatccata acagccactt ttggaccatt tccttttata tgctctactt
    33301 cttttcttgt aacagttcta actgcttctg tatttgaaaa cttatccaat ttatgtttaa
    33361 catcttctaa tttactgtct tctaatgtaa ttataccttt cattgttcca ttatttctaa
    33421 gtatcttagt caaagctctt gtatctattc cttcaagtcc gataacttta ttttgcttta
    33481 aatattggtc tatatcaatt tcacatctaa aattacttgg agaatcactt ttctccctta
    33541 ctatgaatcc ctttacttga actttttcag actctgctga tgataaattt ataccataat
    33601 ttcctacaag aggataagtc atagtaacta tttgaccata gtaagaaggg tctgttaaaa
    33661 cttcaccata tcctatcatc gaagtattaa atactacttc tcctacactt tcttctaaat
    33721 atccaaatgc ctttcctata aaaactgttc catcttctaa tattaacctt gctttcatac
    33781 tacagtacct cctataattc tctttagaca aactaaagaa cattaactaa atataagctt
    33841 ttaaatatct atatctattt ttcactttta cattctagtt ttatacttat ttacatttat
    33901 tttttatctt tttctattat actacaaatt tcttaaaaaa aagaagttat ctcaaaagta
    33961 aaaactccta gaaaaaataa cctctttttt ttatttaata attaaattct taaactatac
    34021 aatcaactta atttctattc agctatattg taaactttta actcatcttt atttaaatgt
    34081 ttcttcttaa cttctactaa agcttttagt gtatccaaag atgtcattat ttcagtagaa
    34141 aattcaatag ctgttcttct tatcttaaat ccatctcttg tagagtcatt tcctttagtt
    34201 ggtgtattta ttactatatc aacttgttta tttcttatag catctaatat gtttggtcta
    34261 gattcttcta ctctatttac tatatcagcc tctattccat tttctcttaa gctattagct
    34321 gttccttctg tagcaacaaa agtatatcct aattctttca tatcttttgc tatctctata
    34381 aactcatctt tatcatgatt atttacagta gcaagtacta ctcctctttc atcagacata
    34441 tgtctacctg ctgccaagaa ccctttatat aaggcttctt ctaaattttc tcctacacct
    34501 agaacttctc ctgtagactt catttcaggt cctagactta cttcaacttt agataattta
    34561 gacattgaga atactggtac ttttactgat actaattttg gctctttgta aactcctgta
    34621 ccatatccta aatcctttaa tttagcccct aacatacact tagtagccaa gtcaactatt
    34681 ggtactccac ttactttact tatatacgga actgttctac tagctctagg gttaacctca
    34741 attatatata attcattttg aaattctata aactgtatat taaccatacc aagcacatta
    34801 aggtctaaag ctattttttt agtgtattct actatctttt cttttatttc atcagatata
    34861 ttttgacttg gatacattgt tgtactatct ccagagtgaa ctccagctct ttctaaatgt
    34921 tccattatac caggtatcaa tatatcttcc ttatcacata tagcatctac ttctatttct
    34981 ctaccagtta gatatttatc tataagtact ggatttttat ggtctctatc aaatgcatct
    35041 tgtaggtatt gagataattt ttcttcgtta taagttattt ccataccttg accaccaagt
    35101 acataagatg gtctaactaa tactggataa cctaactcat tagcaacttc aataccttcg
    35161 ttaagtgacc atacaccctt tccttttggt ctatttatat ctagtctctc tagtaaatca
    35221 tcaaattttt ctctatcttc tgctgcatct atatcatcaa aatcagttcc caatataggt
    35281 atattttttt catgtaaaaa tttagccaat tttatagctg tttgtccacc aaactgtagt
    35341 attacacctt ctggcttttc tttttctatt atacttaaaa cttcttcttc tgttaatggt
    35401 tcaaaataca gcttatctga tgtatcaaag tctgtactta cagtttcagg gttattgttt
    35461 atgattatag tttctatgtc catttttctt aaagatttta cacagtgaac tgaacaatag
    35521 tcaaattcta taccttgacc tattcttatt ggaccagaac ctaatactat cacctttttc
    35581 ttatcactaa caactacctc atcatattgc tcataagttg aataatagta aggagatata
    35641 gcatctatct cacctgcaca agtatcaacc attttatatg ctggttttat attgtataga
    35701 cttctcaatt catataattt ttctggactt atcttcatta ggtcagatat acctttatca
    35761 gagaatccct ttttctttaa ttcaagaaga tagtctttag ttaaatcttc aattttcata
    35821 acttttaatt tttcttcttg ctctactatc catttaaatt tatttataaa ccatttatct
    35881 atacctgtaa tttgttctat catttctact ttatagcctc ttcttatcat ttcagctaaa
    35941 tcaaatagtc tctcatcatc aggtactaca actcttttct ttaattcctc aatgcttctc
    36001 tcctcagatg gtgtgtgaac taatgaatat tttcctgttt caagtgatct tatcccttta
    36061 agaatagcag cctcaaagtt acttcctata ctcataattt caccagttgc catcatctta
    36121 gttcctaatt ttctatttgc ttttttaaat ttatcaaaag gccattttgg tatttttgcc
    36181 accacataat ctatagttgg ttcaaaacaa gcataagttt ttttagttac tgcattttct
    36241 atctcatcta aagtataacc taaagctatt tttgacgaaa ctttagctat tggatatcct
    36301 gtagcttttg atgctaaagc tgatgaccta ctaactcttg gatttatctc tataactgca
    36361 tattcaaaac tatgtggatt aagtgcaaat tgaacgttac aaccaccttg aacttctact
    36421 gcatttaata tatctaaaga tgctttttta agcattgcac attctttgtt acttaaagtt
    36481 tgagttggag caacaactat actgtctcct gtatgaactc cgactgggtc tatattttcc
    36541 atgttacata caactataca atttcccttt gaatctctta ttacctcata ttctatttct
    36601 ttccaacctt ttatactctt ttcaattaat acttgtccaa caggacttaa ttgtagacca
    36661 tgtgaaagta tttctcttaa ttcttcctca ttatcagcta tacccccacc agttccacct
    36721 agtgtgtatg ctggtcttac aactactgga taaccaattg taagtgcata atcaagacct
    36781 gcttgtaaat ctgtaactat atcactaact attacaggct gatttatttc tttcattact
    36841 tctctaaata tatctctatc ttcacctttt ttgatagatt ctactgaagt acctattatc
    36901 tttactccat atttatctag tatgcctttt tcataaagtt caactgctag gtttagccct
    36961 gtttgtccac ccatacctgc aagtaaactg tctggtcttt ctttttctat tattttttct
    37021 ataaattcta ttgttaatgg ttctatatat attttatctg ccacttcttt atcagtcatt
    37081 atagtggctg gattactatt tattaataca acttcaattc cttcctcttt caaagactgg
    37141 caagcttgtg ttcctgaata gtcaaactct gcagcttgac ctattattat tggtcctgac
    37201 cctaatacta aagttttctt tatactatct aatttaggca taaatttttc tccttatcta
    37261 aatggttttt actatctgta aataatacta aatctatatt aatgctaaga atttattaaa
    37321 tatacaatct aaatcttctt ttgttgtgta ttctggaatg tattgaacac tatatattgg
    37381 taattcctta tgtctcattc cttctatagt attgtcattt aaatttatgt gagttacttc
    37441 tgtattttct ggcatttctg aaacattata tccatgattt tgagaagtta taaatacctt
    37501 tccagtttct aaacatttaa ctgggtgatt acctcctcta tgtccaaata caagctttga
    37561 agtctttcct cctaatgcca atgctaaaat ttgatgtcct aaacctattc ctgtcaatgg
    37621 ctttttgcct attaattttt ttatattttc aactatatca tttatatcct ctggatttcc
    37681 tgggccatta gataataaga ttaagtcagg atttatattt aaaatttctt ctactgtagt
    37741 cattgctggt actatagtta tatcacagtt acaagcctta agagactcta gtacactatt
    37801 tttaacacca aagtctataa ctacaacttt agctccagta ccttttatat tttcaatttc
    37861 ttttcttgta acatttttta ctgaatcttt gttacaatgc tcttctaatt tagactgaac
    37921 atcttgtaat gatacatttt ctaaagttat aattcctctc ttagttccat tgtttctaat
    37981 aattttagtt aatgctcttg tatctattcc ttcaagtcct attaccttat tttgctttag
    38041 atatgtgtct atatccattt cacatctaaa attatttggg ttatcacttt tttctcttac
    38101 aataagtcct tttacttgca ccttatttga ttcaacatct tctaaattta ttccatagtt
    38161 tcctataagt ggataagtca tagtaactat ttgtccataa taagttgcat ctgtcaatag
    38221 ttctccatac ccagccattg aagtattaaa tacaacttct ccaatagctt cctttagata
    38281 tccaaatgct tttccttcaa atactgttcc atcttctaaa attaatttcc ccttcatgta
    38341 agctacctcc caaatatttt atctaagcat tttctatttc tcttacaata ttaatagctg
    38401 attctcttgg attttctgct tttgttatag gtcttccaac aactaaataa tgagcacctt
    38461 tttttattgc atctgatgga gttacaactc ttttttggtc tccaacctct gctgatttag
    38521 gtcttattcc tgggcaaact gttataaaat cagttccaca tgcttctact attttatcta
    38581 cttcttgcgc tgaacaaaca accccatcta ttcctgattc ttttgccagt ttagctctct
    38641 ttatagctaa ctcttctgtt gtcatattac atcctatgtc ctgtaaatct tggttgctta
    38701 atgaagttaa aactgttact ccaactacta atggcttttc tatatttaat ctttctgctt
    38761 cttccctagc aattagtgca cattgtctca ttccttccat atctgataca tgtattgtca
    38821 ttaaccaaac attatctctc actgcagctt tcactgcact tttcatagta tttggtatat
    38881 catggaattt aaggtctaaa aatatttttt tccctttttc tttaagataa tctattgttt
    38941 taccttttgt ggcaacgtat tgctctagcc ctaccttaaa tatgtctaca ctatcttcta
    39001 atctgtctat tagttcttta gctttatcaa attcgtcagt atcaattgca actatcaatt
    39061 tttctttacc atttatcatt tttacatctc ctttttaata aagcctaaat aatcataaaa
    39121 aaatcctcac accaaaagag gcataaggat tttatctata cataactcta ttcattagct
    39181 atgagatatc tttcccagtc tctctggact agtttaaaag actttattat atttaaattt
    39241 ttattttata ctcatcaaaa acctctagaa gatttactag aggttatata ctaacattat
    39301 ctataaccct ttagtctctc tggactactt taaggaattt agctgtaata tttttcttta
    39361 tttatataac tttatgtatc tataggataa tttaaagttt tatctttttt ataattaaac
    39421 cactaattgc tttttatgtc aataagtctt tgaattattc ttcatcttta tcaaacaatt
    39481 ttttctcaaa atatgattct gcattatata ttgcagcaac tggattatgc ccagttactc
    39541 tatctttgac aactaaagtt gttataggag cttccatata tttcatggct aaagtatcgt
    39601 gaccaacaca taatccaaga attacattaa ggtcaacctt agcttcattt aaaaataaag
    39661 cttgtcctat tggattgcac attacctcat ctgaacactt tgatattgtt tgagatgctt
    39721 ctagtccaat tgtactctta ggcattgccc catttttaca tataacagaa aatacttcaa
    39781 acccatgatg ttcaaatatt ttttgcactg tttgcatttc tctttttagt ccaatacaaa
    39841 atgctatgcc aatcttttta taaccacact ttttagcaaa ctctataatc tcttttagtc
    39901 tagtatattg acaatacccc tctgcttcaa ctaatccagc attataagct atttttctat
    39961 tctcttcttc ttcataaagt tgctttgctt tttgttgtag ttcaatttct ctagttggac
    40021 atatcttcat taatttatcc atatttccat tagaacagcc tctaagatta cattttccac
    40081 acttatacat atccaatatc cccttccaaa attttattca tcttgcgatt cttctttttc
    40141 tagagttaca ttttcttctt gggtttcttc ttctggtttc acatgctcga ctacagttat
    40201 aaaatatgat tttcctattt tattccttat aggaagtttt tcatatgaaa catctatttt
    40261 attttttata caagctatat caaaatgtct taaggctaca cctaagctaa ccttattcat
    40321 atcttcacag tgtttcttag gtctcttttc aaataaatga agacctcgtt catccttatg
    40381 gaaataccac ggttgagaat tttttacaga tggagctctt cttgtcaatt ctaagatatc
    40441 tttccacttt ctattcctat ttatcttatt tatcatatct tttactggct ttctatcagg
    40501 tctagcatat ctcgtcctaa acaactcttc attttttgct ggatatccaa atgctattat
    40561 tatataagga ttttctattt tgttttcatc atcttcttct gtttttattc cagctagata
    40621 atctaaatca tcttcctcat cttcttcaaa attcttattc ccaatatcta caaattccaa
    40681 gatatcttct ctctttaagt ttccttccaa ccagcaagta gctattccca atgttgtaag
    40741 gcgcaaaact acaccttctg tagcaaatcc tatattctgt aaatagtcct cacctttgtc
    40801 tgatgtaact actatataat gaggtgcctt tactttacat tccttcccca tcaaaaaatg
    40861 tattatatga cctctatcaa caacatgtgc tttaatattt aaatcttcat ttaagtattc
    40921 taaattttta caacttcttt ttatttcttc catcaatggc ttttttatct ttttactgga
    40981 aaaatttctt acagattttc tataaaaaat ggcttcataa agctccattt atgccctcct
    41041 aactgaaatc ttctgacgta taaactctat aaatttatat aaatacttta tattttttat
    41101 agttttattt cctttttcct cctgccctat tttgaaattc ataagaatat ctactctagt
    41161 ttaatatagc attcaaaagg cttaaattct accttatcaa tgtctataca ttagcataat
    41221 ttttttattt gattttctat gctaatttaa ataacaacac tagctattac ctttttaatt
    41281 aagctttgcc agaaaaattt tttttcattt ataaatatct atctcattat tatattataa
    41341 gtttttttcg gtattcttgt atagcttttc actaaaaaag tatttttatt tctactttaa
    41401 tattattatc aacaaaaaac ata
//
LOCUS       DAYAPA010000022        40866 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.022, whole genome shotgun sequence.
ACCESSION   DAYAPA010000022 DAYAPA010000000
VERSION     DAYAPA010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 40866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40866
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.022"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            194..853
                     /gene="cprR"
                     /locus_tag="ACGI59_001047"
     CDS             194..853
                     /gene="cprR"
                     /locus_tag="ACGI59_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CprR"
                     /protein_id="HFG9738851.1"
                     /translation="MSKILVVDDELDMLKLIENVLKRDGHEVKVISDVEKAMNMDFNY
                     FNLIILDVMMPKMDGFEVCKTIRNKVDCPILFLTAKNMESDIMYGLGIGADDYITKPF
                     GVGELRARITAHLRRESREKKNLLTISNVQFNFLGKEVSIDGKKINFTKSEYLICEFL
                     AKNKGQVFSKERIYESVYGFDGESDISTITEHIKNIRSKLKQYDIYTIETVWGIGYKW
                     V"
     gene            1064..2122
                     /locus_tag="ACGI59_001048"
     CDS             1064..2122
                     /locus_tag="ACGI59_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HFG9738852.1"
                     /translation="MDRLKNIRKSLLQYTFLFLLIGITIYLVFKTLDIKMLSNVMVMV
                     DKKFLFIGALAIMCHIMLEGVVMKIIIESTHKVNIRFIGFKLAIMGFYYNLITPFASG
                     SQPVQIYVLKKCKMPLSKASAVVTNKSIIFQIVVTFYCTILVLMNFAMLEKQMKMIMP
                     LVFLGIAINSFVLIMIILVILNPEKIKYFMRITIKHLSKIKFLKFLDNKIESIESFID
                     DYSKAINFFAKNKKVLISTIIVTFIQLSAYFSVSFWIYKAFNLQGYSYIYILTLQAFL
                     YMAISPIPTPGNVGANELAFFTIFKSVFPQPLMGYAVFLYGGFMYYLILIGSGIFTVI
                     THYRMKNRIGRNIVLSEN"
     gene            2373..4517
                     /locus_tag="ACGI59_001049"
     CDS             2373..4517
                     /locus_tag="ACGI59_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:2787..2789,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase H subunit alpha,
                     selenocysteine-containing"
                     /protein_id="HFG9738853.1"
                     /translation="MEKKILTVCPYCGAGCQLYLVVKDNKILRAEPANGRTNQESLCL
                     KGRYGWDFLNDPKILTPRLKKPMIRKQGVLEEVEWDEAINYTASRLNEIKEKYGPDSI
                     MGTGSARGPGNEANYIMQKFMRATIGTNNVDHCARVUHAPSVAGLAYSLGSGAMSNSI
                     PEIENADVLFIFGYNGADSHPIVANRIVKAKKNGAKLIVTDPRVTESARIADIHLPIK
                     GGTNMVLVNAFGNVLIEEGLYNKEFVQNHTQGFDEYKEIVKPYTAKYAEKITGIPEEL
                     IRKAMREYAKGKKAMILYGMGVCQFGQAVDVVKGLASIALLTGNFGRESVGIGPVRGQ
                     NNVQGACDMGALPNVYPGYQNVTDDKIREKFEKAWGVKLSPNNGYSLTQVPNLVLKEK
                     KLKAYYIFGEDPVQSDPDASEVREALDELEFVIVQDIFMNKTALHADVILPATSWGEH
                     EGVYTCADRGFQLMRKAIEPQGDVKPDWQIISEISTAMGYPMNYKNTKEIWDELRQLC
                     PSFLGATYEKIETQGCVQWPCKSESMEDKGTMYLYEGQKFSTPNGKGNLFAAEWRPPM
                     EVEDDEYPFSLCTVREVGHYSVRTMTGNCRTLSSLEDEPGRVQINSNDAEKLGIEDDE
                     LVRISSRRGSVITRATVTDRVKEGATYMTYQWWVGACNELTIANLDPISKTPEYKYCA
                     VKLEKLEDQELAEKCVREEYQSLKDKMTATNI"
     gene            4566..5399
                     /gene="fdhD"
                     /locus_tag="ACGI59_001050"
     CDS             4566..5399
                     /gene="fdhD"
                     /locus_tag="ACGI59_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HFG9738854.1"
                     /translation="MNNIYSKTKLQENSKDKVFENSYSLVDYKYLNSFNVEVSKINEH
                     EVSIDNEEIIAEYPLSFILNDKYVNTFLCTPYNLEELIVGFLKTKGYIENKKSLLKFE
                     INESERFAKVTIQEKQKNQDEQIIFLNSLDYITCAPVKSNIKINYDTIYSIMDKNLNS
                     SELFKNTGGVHSVSVFNFDKKIVTCEDVARHNAMDKAIGFCVLNDITLKDKIVAVSGR
                     ISLEMILKAAMTQIPIVVSKSAPTNLSIELSKKLNITLIGFVRGRRMNIYANPQRVIK
                     K"
     gene            5410..5583
                     /locus_tag="ACGI59_001051"
     CDS             5410..5583
                     /locus_tag="ACGI59_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9738855.1"
                     /translation="MCTKKEEQRYSKCEICGVLIDDTDKVYNNTYNLCKKCKRNISTE
                     KIVKYINIYDESK"
     gene            5641..6192
                     /locus_tag="ACGI59_001052"
     CDS             5641..6192
                     /locus_tag="ACGI59_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFG9738856.1"
                     /translation="MKNKIGSFVIADPNKCTGCRACEVACFTMHNQHNNVGYTVGTVD
                     IPVIPRLYLVKGDNFCMPIQCRHCEDAPCLNSCPQKAIVKENNIMSVNEEKCIGCKTC
                     LLACPFGAIDLLPQYEDGKEVEQVILDENKKIAYKCDLCKDNEKIACISACPQEALKL
                     VTPIDDKKAKNRQAALSLLLTNK"
     gene            6217..7593
                     /locus_tag="ACGI59_001053"
     CDS             6217..7593
                     /locus_tag="ACGI59_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016695 - oxidoreductase activity, acting
                     on hydrogen as donor [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase, group A"
                     /protein_id="HFG9738857.1"
                     /translation="MIINIDKDLCTGCRECSKVCPVNAIEGEEGKPQEINLDRCVMCG
                     QCVQTCKSYASVIDEGFEFLQEKKQEREIPESINEPIFAAYNVCDIDKVKKALSDPNV
                     FTMVQCAPAVRVALGEDFGYYLGYLGAGKMAAALRALNFDRVYDTNFAADLTIMEEGS
                     ELIKRVTEGGTLPMFTSCCPAWVKFMEKNYPKLTNHLSSCKSPQQMGGAIFKTYGAEI
                     NNVDASKIFSVSIMPCTCKKYECDREEMDSSGYRDVDVVLTTRELAYLIKDMGIDFKN
                     LREEKFDSPLGSYSGAGTIFGVTGGVMEAAIRTGYELITGESIPDVEVKQVRGENGFR
                     KSTIKIGDLDLRVGVVSGLKNVIPVLEDLEKGKLDVDFIEVMTCPVGCVSGGGQPKIL
                     LEEYKELAYENRTKATYVHDEHLAIRKSHENPDIKKLYDEYLVEPLGEKSHHLLHTTY
                     CIGKEVAK"
     gene            7593..8108
                     /locus_tag="ACGI59_001054"
     CDS             7593..8108
                     /locus_tag="ACGI59_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFG9738858.1"
                     /translation="MNYFVIADPDKCIGCRTCMIGCVVAHSDEDIFYQNPDEINFNPK
                     LSVIKTKEVSAPIQCRHCEDAPCAKACPNGGIVRVGNTIKINEENCIGCKTCMLACPI
                     GAIDIVTLKDVDEGKLCFRERMTANKCDFCIESPEGPACVNVCPTKAFTIVKDEDIDS
                     KVKNKRKLAIL"
     gene            complement(8186..9469)
                     /locus_tag="ACGI59_001055"
     CDS             complement(8186..9469)
                     /locus_tag="ACGI59_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugated bile salt MFS transporter"
                     /protein_id="HFG9738859.1"
                     /translation="MNTLLNNKFSIKNFSNAWVIVLGCMLIQAVPFSIVASIQPQFIS
                     YVVEGEGFSLASFSLIFTIGTIASAIASPFIGVLFSKVNIKILYLLGCIISSIAFATF
                     SICTELWQFYTVSCIVQIGATIISAIGIPVLISAWFDEESKGKALGFAFAGGSIGSIF
                     LQPLAVRLLATQGYSRSYLIFGIIALIVGIPVILFILRMPKDINEKVKSKGKKEKASD
                     TISPSVWGYTLKEAKKIKYFWIMTIGFIFVGIYVSSLSVQYPTYLRNYLNPILIGTVG
                     STFALFALIGNLFGGLLFDKFGQTKPLIISFVLVLIATLSLLLVDKSSTFAFVFAILK
                     GLSVFAYMIGPAYLTGSFFGQKEFGSIYGIVQLIFAVGFALGSTLFGLLVDNFGYRFS
                     WSIIILSVIIAYTLLIITSIGMKKLNKERLDKLMQ"
     gene            9657..10079
                     /locus_tag="ACGI59_001056"
     CDS             9657..10079
                     /locus_tag="ACGI59_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3783 domain-containing protein"
                     /protein_id="HFG9738860.1"
                     /translation="MSFEKINNNNLDKIHNRKCIMLVNFNKKELSLIKNVSNFAGIRD
                     HIILSDKNGENIIRDILDGNISNECENGIKSKSVIFNNTPSTQVNAFLEGLKKVRINR
                     PFTAMVTETSIDWTLNNLIENLVQERNAMSLGKTIEHK"
     gene            10181..11119
                     /locus_tag="ACGI59_001057"
     CDS             10181..11119
                     /locus_tag="ACGI59_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFG9738861.1"
                     /translation="MKVLFTINYGKEKFDKIRELGYDVIHYSEDSIENNEDVNTADVL
                     VTYNPFERLDISKMKNLQYIQTTSVGIDQLPKEEVLKRNIKIANNRGGYSVPIGESIV
                     LYILEIYKNSAKFFKQQQNKEWKMDFSVSELYGQRVGFLGTGTISSEAAKRLKAFGVE
                     IWGVNTDGSSKKYFDKCFATEDMNIVFEECDIVVVAMPSTKSTDGIVNKDMFKLMKDG
                     SVFINVGRGNTVNQKDLEECVEKFRGVALDVFESEPLNKDNKLWECENVIVTPHNSWV
                     SDKNKERTFNMVYNNLKHYIMENKPVDNVVDISRGY"
     gene            11305..13329
                     /gene="ligA"
                     /locus_tag="ACGI59_001058"
     CDS             11305..13329
                     /gene="ligA"
                     /locus_tag="ACGI59_001058"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFG9738862.1"
                     /translation="MSVKDEINKLIETINYHNERYYNQDSPEISDYEYDKLMKELISL
                     EEEHPELKRIDSPTNRVGGKPLDKFNQIVHKTPMLSLSNAFSEQDLRDFDKRVQEYVG
                     ENVEYVVEFKIDGLSVGLTYNNGEFEKGATRGDGVVGEDISQNLMTVKSIPLKINDKK
                     ELIVRGEVYISKDNFRKVNEQQEEQEQPLFANPRNLAAGSLRQLDPKLTAKRPLDIFV
                     FNMENIEEYDLESHSESLEYLEKLGFSVSQSYKVCKSIDEVIEHIEYWTDNRGNLDYE
                     IDGMVIKVNNIKQRDEMGYTAKSPRWAIAYKFPAEKKKTKIVDIIVEVGRTGTITPSA
                     ILEPVRLAGTTVSRATLHNEDYIREKDIKINDTVLVQKAGDIIPQVLEVIKEERTGDE
                     IDFKMPEKCPVCSEPTIRLEGEAAVKCINMSCPAQIRRGIIHFVSRDAMNIDGLGESI
                     ITLLLNEKIIQDVSDLYYIKKEDVVDLERMGEKSADNLINSIEKSKENDLWRLINGLG
                     IKFIGVKAAKILAYNFKDLDEVISATSEQLEELEEFGSIMANSVVEFFKEDKNMNVIN
                     KLKNVGVNTKSLDNTGEKVEKIFEGMKIVLTGTLPTLKRNDAKDMIEKRGGKATSSVS
                     KSTTFVLAGEEAGSKLTKANDLGIKVIDEERFLELLKLSSKDEVKAVLES"
     gene            13489..14838
                     /gene="rph"
                     /locus_tag="ACGI59_001059"
     CDS             13489..14838
                     /gene="rph"
                     /locus_tag="ACGI59_001059"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFG9738863.1"
                     /translation="MTRSYDRSNEQIRPVKITRDYTKYAEGSVLIEMGETKVICTASV
                     EEKVPPFLRNSGTGWINAEYSMLPRATQQRKIRDASRGKIDGRTQEIQRLIGRTIRSV
                     VDLNKLGERTIWVDCDVIQADGGTRTASITGAFVAVAEAIYKLYKAKTIKEMPITNFV
                     SAISVGIVNGIHMLDLCYEEDSKAQVDMNIIMTDKCEFVEVQGTGEEKPFSRADLNLL
                     LELGEKGNKELIRLQREALGEISDEILGMDYGNEVVIATNNAHKLEEIGEILKDFEYK
                     VYSLKDVDLAGIEIVEDGKTFEHNALIKARTIAKKTKLIAISDDSGLEVDALGKKPGV
                     YSARYAGEHATDEENRKKLLKAMQNVPMSKRNARFVSAIAVVFPDGKEFVVRGICEGM
                     IGFEEKGKNGFGYDSLFIVKGYDKTFGEIPSVIKNSISHRANALKLMKQEFIKRVVK"
     gene            14838..15308
                     /locus_tag="ACGI59_001060"
     CDS             14838..15308
                     /locus_tag="ACGI59_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFG9738864.1"
                     /translation="MKIGIVSDTHMMIKNMEKTIPYLKECDLIIHAGDNFTDSRYIHS
                     MTNVGIIAVKGNCDFDAVEEEVVFEVANKTIFLCHGDKYGVKYGTNMLEKKATEVDAD
                     IVIFGHTHTPFREIKDGVLYINPGSTSLPRGVSYKSFVIMDIEEDDIKIEEIRI"
     gene            15502..16788
                     /gene="tig"
                     /locus_tag="ACGI59_001061"
     CDS             15502..16788
                     /gene="tig"
                     /locus_tag="ACGI59_001061"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFG9738865.1"
                     /translation="MKAELIKKEGNKVTLKITVDNNKFEAAVNKAYNKSRNKYNIPGF
                     RKGKAPRIVIETQYGKGIFYNDAIEILFPEVYPEAIKELDIDPIDNPDLDIEEISKDN
                     GLVMVLNVEVKPEFELGNYKGIEIAKVENTVSDENVDAKLQEMVEKNARLVSVEDKAL
                     EDGDTAIIDFEGFENGVAFDGGKGENYNLVIGSNTFIPGFEEQLVGKKAGEEVEVNVT
                     FPEEYHSQDLAGKPVVFNVKINDVKVKELSALDDEFAKDTSEFDSLDELKADVRAKLE
                     EEAKNRADAETRNSVVEKVAENTEIEIPEVMIQHQIDNMLNELNYQLQYQGFGLQQLL
                     EMTGRTMEELREERKEDAKKLVKSSLVLEAITKAEGIEATEEEFKAELEKMASAYNME
                     VEKIEASLRDADKEDIKGQIKIRKTIDLLVDNATIA"
     gene            16938..17522
                     /gene="clpP"
                     /locus_tag="ACGI59_001062"
     CDS             16938..17522
                     /gene="clpP"
                     /locus_tag="ACGI59_001062"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFG9738866.1"
                     /translation="MALVPVVVEQTGRGERSYDIFSRLLKDRIIFLGDQVNDATAGLI
                     VAQLLFLEAEDPDKDIHLYINSPGGSITSGMAIYDTMQYIKPDVSTICIGMAASMGAF
                     LLAAGAKGKRLALPNSEIMIHQPLGGAQGQATDIEIHAKRILKIKETLNEILSERTGQ
                     PLEKIKMDTERDNFMSALEAKEYGLIDEVFTKRP"
     gene            17541..18791
                     /gene="clpX"
                     /locus_tag="ACGI59_001063"
     CDS             17541..18791
                     /gene="clpX"
                     /locus_tag="ACGI59_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HFG9738867.1"
                     /translation="MSKYEEKRQLKCSFCGKNQDQVRRLIAGPNVYICDECVELCDEI
                     IQEEIEDTIDEDTTSLPKPKEMMEILNDYVIGQEKAKKALSVAVYNHYKRIYSKKSSS
                     KDIEIQKSNILLLGPTGSGKTLLAQTLARTLNVPFAMADATSLTEAGYVGEDVENILL
                     KLIQAADFDIEKAERGIIYIDEIDKIARKSENPSITRDVSGEGVQQALLKILEGTVAN
                     VPPQGGRKHPHQEFLKIDTTNVLFILGGAFDGLEKIIQKRGGDKTLGFGAKIESKKEL
                     DLGKLYEKVLPEDLLKYGIIPEFIGRIPVLATLELLDEDALMQILQEPKNALVKQYKK
                     LLELDDVELEFEEGALRAIAKKAIERNTGARGLRSIVESVMMETMFEVPSRDNIKKVI
                     VTEKSVNEDSVNPIIVLKDQEESA"
     gene            complement(18862..19389)
                     /locus_tag="ACGI59_001064"
     CDS             complement(18862..19389)
                     /locus_tag="ACGI59_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HFG9738868.1"
                     /translation="MSEIILLFLIFLKIGAFSFGGGYAMLPFIQQEFISKYHFITNQE
                     FLDLLAISQSTPGPVAINSATFVGFKTAGFFGSLAATIGVTIFALIGLTIISKLFSKF
                     KNNKKVENLLTVLRPITLGFILSAAFSSAKEVNWDLYGIIAFIASFYLLYKGKIGSIA
                     IVFVFGILGILLTFL"
     gene            complement(19377..19889)
                     /locus_tag="ACGI59_001065"
     CDS             complement(19377..19889)
                     /locus_tag="ACGI59_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HFG9738869.1"
                     /translation="MLKLFLTFLKIGAFTFGGGYAMVPLIQDELVNKQKLLEQEEFID
                     YLSIAQSYPGVLAVNISVLLGYKLYGIPGVLICTLGSALPSFCIVLVLSYLYFKNSSS
                     KILDGFFKGVAPVVIALILYSFTSMFKKLPKSKTNILLLIIAVLSVGVFNISPIYLII
                     GGGVYSLCQK"
     gene            20069..22432
                     /gene="lon"
                     /locus_tag="ACGI59_001066"
     CDS             20069..22432
                     /gene="lon"
                     /locus_tag="ACGI59_001066"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HFG9738870.1"
                     /translation="MEQNYTKIDHELPLIPLRGLAIFPYMILNFDIGREISLKALDQA
                     MMDEELIFLTSQKEAEVDEPGEEDFYHVGTICKVKQMIKLPGDTVRVLVEGVSRGRVK
                     KIEQEDGYFRAVIEEIVFDSDNLDSETEVEIEAFVRNVFDAFEEYINIGNRVSPEILI
                     SLADIEDVDRFIDTIAANIYLKSSQKQEILEEFDIRKRLELIYSILLEEIDILKIEKK
                     ITLRVKKQMNKVQKEYYLREQLKAIQKELGEEEDINSEADEYREKLKKIKAPKTTKEK
                     IEKEIDKFSKISSMSPDVSVSRNYLDTIFSLPWNKETKDKLDITKAKDILDEDHYGLE
                     KVKERILEYLAIRTLAKSLKGPIICLVGPPGTGKTSIVKSIARALNRKFVRISLGGVR
                     DEAEIRGHRRTYVGSIPGRIINGVKEAQTKNPVFLFDEIDKMAADYKGDPASAMLEVL
                     DPEQNKDFVDHYLEIPFDLSKILFVTTANSLSNIPRPLLDRMEVIEVSGYIEEEKLNI
                     AKKYLLPKQIKEHALKENFIKIDDETLRSIINHYTREAGVRTLERTIGKICRKVAKKY
                     VEDPTLEEVVINKSDLETYLGKDMFKYQLAEVNPQIGLVNGLAWTEVGGVTLEVEVNV
                     LKGKGEIVLTGKLGDVMKESAKTGISYIRSIVDKFDIDPEFYKTNDIHIHIPEGAVPK
                     DGPSAGITMALAVISALTKRPVPGNIAMTGEITLRGRVLAVGGVKEKLLAAHRAGITK
                     VLIPKECEADLDEIPENVKEKMEFVLVEHMDEVLEQALLKSGENNEN"
     gene            22422..23033
                     /gene="yihA"
                     /locus_tag="ACGI59_001067"
     CDS             22422..23033
                     /gene="yihA"
                     /locus_tag="ACGI59_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFG9738871.1"
                     /translation="MKIRSSEITMSAVNKSQYPAEGIPEIALAGRSNVGKSSIINTLL
                     NRRNFARTSQTPGKTRTINFYLINNEFYFVDLPGYGYAKIAKSEKEKWGGIMERYLES
                     RQELCSIFLLVDIRHEPTADDKLMYEWIKHFGYNCVVIATKADKISRGQYQKHISIIR
                     KKLQMESSEKVIPVSSLKKTGVEELWEEIVNQYNQHGYEITVD"
     gene            complement(23298..24668)
                     /locus_tag="ACGI59_001068"
     CDS             complement(23298..24668)
                     /locus_tag="ACGI59_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFG9738872.1"
                     /translation="MKQKWIVLIIICIGVFMSTLDGSILNIANPTIAADFKINMSQIQ
                     WVVTAYMLVVTATMLFFGKLGDKVGSNRLYTLGFFIFTIGSFLCSMSNNLSTLISSRI
                     FQAVGASILMATGLGIVSNAFPANEKGKAIGITGAVVGIGNMSGPVIGGIILEHFGWP
                     SIFIINIPIGIIAVFLGIKFLPKPVLDEQNKSFDIPGLLLFASCTTLILLAMNEKGNT
                     RLYLGITALIIFLLLALREVKFEQSFIDLPLFKNRNFTVGNIIGVACYFPQMAVSFLL
                     PFYLEQLKNLSPMMAGYVMTVHPLIMVLIAPIAGSLSDKHGAKNILTASFSFMTISLV
                     GMALLKADSPLYLLIVCLVIFGLGLGAFSSPNNSSILADVPPQKQGYGGSFLATIRNL
                     SFALGTAFFSSFFAQSLTYNQKFKSHTSAYVIASNQSYWIAASVCFIGLILTVFFMRK
                     TDKSIS"
     regulatory      complement(24843..24958)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            25246..26325
                     /locus_tag="ACGI59_001069"
     CDS             25246..26325
                     /locus_tag="ACGI59_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFG9738873.1"
                     /translation="MEGKVKKAFPGGNTSQGSYSFFDYIIPENVKRVFCLKGGPGVGK
                     SSLMKKIYKEFLHRGYDIDLYHCPSDPSSLDGLVIKKLGVVLMDATAPHTMDPKLPGA
                     LDEIINLGDYWNTEELEKNKQEISVYDKDISNSFKRAFKFLKSAEPIFRDIEEKYSDC
                     MDYGKVNLVTEEFIKRLFDGVKSTGNLKREKHLFGSAITPVGCLDYTENILKDVKKVY
                     YLDGEIGTGKTTLLNKVYKKAIEKGLNVEVYHYPLIPEKIETVLLTDLDIGITISTTF
                     KEEDTIDLNQFLNREKLLKYEEDIKFDEKVLDDLICWAVLNLKRAKSKHDIIESYYSP
                     NMRFDEVGKLRDKLIKRILKYEENL"
     gene            26716..27396
                     /locus_tag="ACGI59_001070"
     CDS             26716..27396
                     /locus_tag="ACGI59_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HFG9738874.1"
                     /translation="MKKQYVFIILASTILGILVGTIFRDYSNEETVYTSKDKSIRKEI
                     KMTRKSIKELTKEKDKLDKEINSMKQNQKNSEEISEIETLRDSLSYSDVKGSGIILSI
                     DAIDEKTGNIANSIDYNKILVKVINELKVNGGKYIEINGQRINQYSEVVLAGSHININ
                     GVPVAQPYEIKCIGDKEKLSNYVDKGSKYLDSIQTNYPMKISIKTEENISMKKMNIPN
                     KLKYIKGD"
     gene            27389..28768
                     /locus_tag="ACGI59_001071"
     CDS             27389..28768
                     /locus_tag="ACGI59_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HFG9738875.1"
                     /translation="MIKIILEESSLPMLIPEKLAKKYNVFPVEFQDDNLVVEIEDEDI
                     YALQDLRLATGKEIILKKEKKELISEKIEKYYNSFELDEDYAKKLFENLLEKAIKENA
                     SDIHIEPFKEHLIVRMRVDGELKEVSKFLVDVYPSLSTVIKLKASMDITEKRLPQDGR
                     VDIKLGGNLIDIRVSSIPTVYGEKIVLRILNRDTFLKDKLELGFSEEAIKSINRIINK
                     RAGILLVTGPTGSGKTTTVYSLLNDLKGINKNIMTIENPVEYKMDGISQIQVNSKVGL
                     SFDVGLRAILRQDPDIIMVGEIRDAETAKIAVRAAITGHLVISTLHTNDAVSSIARLL
                     EMQIPPYLLNASLIGVISQKLVRKVCNHCSHEIMIKDNFSGDVNTKVAVGCEKCNDKG
                     YFGRTAIYEILEINDDIKTCIRNMEDSSTIKDVAQNNGMITFEDSCKRLINEKITTLE
                     ECLVVNEMI"
     gene            28939..30024
                     /locus_tag="ACGI59_001072"
     CDS             28939..30024
                     /locus_tag="ACGI59_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HFG9738876.1"
                     /translation="MRLSKNIFYNLEKFYLVLNRISDKEIRVICKEIGILLESGCEIT
                     KIFEIIESQSSKKVKNLLSIVSNHIQKGNSIAESFQITGIFSKFFISMIKAGETSGNL
                     DIIMSDLSNYYDKEYKLKMKIITISIYPIILIILSILSMLFIFVFVIPNFQVVFTNNG
                     IEPPLITRVLMGISTVVTNNLAYIIFSFILFSIGSIYFFITNDNIKKLINSLKFKIPF
                     IKKINQLVATTRFSRALSVLISSGIQIVEAIDMSASVIDNEFIYERLKISNNYIKKGN
                     SIGKSLNLANIFPKLFISMANTGEESGKLDKSLNVINKFYENELNNKIEQIMKFIEPM
                     LIVFMGLIIGTFILAMVVPMFDAITNF"
     gene            30078..30437
                     /locus_tag="ACGI59_001073"
     CDS             30078..30437
                     /locus_tag="ACGI59_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HFG9738877.1"
                     /translation="MINLINKKRKGFTLVEMIVVVTILGVISSIALVKYSKVQESAKL
                     NADYTNAANIVTAASMAINDDEKTIDSLSVETLKEKGYLNTVPVPQSTSGKFELVIND
                     SGTDISVNINSKQFYPK"
     gene            30490..31362
                     /locus_tag="ACGI59_001074"
     CDS             30490..31362
                     /locus_tag="ACGI59_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HFG9738878.1"
                     /translation="MAIIKKILNLEINDDYIRLVFLRKSHKKVFIDKSIIKEINFDIK
                     NDSNSIEKIKYIEDVVRFVKEEIGKSKILYKNLYFNIQIQDIVIRNVEVLNTKKKRDI
                     LSMIEFEITQYIPININNYSIKYKVINSKNDKLNIQVILFPKNIIQLIKNISENLNMN
                     PKTININFDILQKLINLNLIENFSEEGVFIECKKSQLIINITKDKKIRETYLLSKEGK
                     SYEFIKRLLRGFKYVYYYGIENCFIEEYFKYTDKSIEVNPLRLKDKVKVENNFDADLE
                     DNRINYINSIGMII"
     gene            31363..31806
                     /locus_tag="ACGI59_001075"
     CDS             31363..31806
                     /locus_tag="ACGI59_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9738879.1"
                     /translation="MKVDKTINFLNGFKKTKPCNKIYKIIIVLILTCILFKSFMYVKS
                     INELEDYISQNKLVDKSKNTLPTTDKTREKFSKIKLNEIKKIYETIGINSITKISIDK
                     NKLEMEGVCSDLDVLNNLRKIKYIKYYYIDSIVRDGEYYRFRIKS"
     gene            31821..32405
                     /locus_tag="ACGI59_001076"
     CDS             31821..32405
                     /locus_tag="ACGI59_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9738880.1"
                     /translation="MRLNNREKNLLKILLILVIILASDIAVSYLEENLTSLKNEKQNI
                     INKINNQDNYQIKDNKHIDDKYIESVELNKNEELKNNFNNSFLDKKTNKDIVLLVENE
                     LRDIVNVEYINQESSLDNHNKKQVNLEIKVNGELANVLKLEGVMKKLRLNNKLEKIEI
                     NKLKVNNFDVQNIPNEYKQEKDNIVECKMTVRVI"
     gene            32658..33695
                     /locus_tag="ACGI59_001077"
     CDS             32658..33695
                     /locus_tag="ACGI59_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HFG9738881.1"
                     /translation="MLGRNELCPCGSGKKYKKCCLNKDIVSERACRKTELSQKQYTDL
                     YSKLYDYSRQDKFKEECEKAKEMFYIMQNDSVNEKFERFFNTYLIQDHIMENKKVMTV
                     GFLEENGANLSQSEVSILKSMFESYVSIYEVKEVSAGKIVLKDCLNGNELCTEDVKLL
                     RSFKIGSCMIARIVDIEGTNILIDITISISNEVKDIILKDIMNLFNQYQDVYKDMKSF
                     LIYHTHILYKYIQQLLDPSIAEYLKRERDKNDTKVNSENELAEEECKVLDTLKENVEK
                     ENIDACIEFWNEYKKNHENIKGAENGWAAAVEYYVKKEAGQPITQVQISKKYDISSST
                     LGKRYKDLKIS"
     gene            33776..34141
                     /locus_tag="ACGI59_001078"
     CDS             33776..34141
                     /locus_tag="ACGI59_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9738882.1"
                     /translation="MSLSLGNAIAVSTNFIKSNPAVPVYNGEEIYAVIYHIEIGYNNG
                     VSPDTKQYIIIDYKEFDLSMDKKNELINIAKEYCKSKGIVENENLEINLLDNEEAENF
                     LEKVFNNMKVIRGLITKKG"
     gene            34146..34463
                     /locus_tag="ACGI59_001079"
     CDS             34146..34463
                     /locus_tag="ACGI59_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9738883.1"
                     /translation="MDYIGIENITPYENTYEFSVYEYDDEITLGSEKLYVCELRVVLI
                     KVNSLYVERLHKSVEAMVLVKNLKKDLDKTLVVNKIKNFVLDEIWVENLVKENIEVIF
                     VES"
     gene            34511..36115
                     /locus_tag="ACGI59_001080"
     CDS             34511..36115
                     /locus_tag="ACGI59_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFG9738884.1"
                     /translation="MLRVSNVKLGIDKDISLLKDVILKKLKIKEKNLVKYSIYKESID
                     ARKKGKMEFVYSVDVEVKDENKILNSKIKDVTKIKEIRYVNVPVGDKKLKNNPLVIGS
                     GPAGLFAGLLLAQMGYEPIILERGLDVDSRTKDISDFWTTRKLKNNSNVQFGEGGAGT
                     FSDGKLTTRIKDIRCRKVLEELVQFGAPDEILYSHKPHVGTDILKNVVKNIRNEIISL
                     GGQVRFNSKVTDIVVSNDSIQSVIINDTEKIDTDTIILAVGHSARDTYEMLYSRGIKI
                     VQKPFAIGARVEHPQDLINKAQYKDFYNHPRLGAADYRLIEHVSNGRTAYTFCMCPGG
                     SVIASASNDFEIVTNGMSEHARDKINANSAFLVNVTPEDFKSDNPLAGVHFQQKYEKL
                     AFELGGKNYNAPVQLVGDFINNKTSLNLGSVEPSYRPGYTLTNLSECLPSFVTETMRE
                     AFISLDKKLNGFAMYDAALTGVETRSSSPIRIVRDENTLESVSIKNLYPCGEGAGYAG
                     GIVTAAVDGIKCAEKIIQKYTNNTKI"
     gene            36239..37867
                     /locus_tag="ACGI59_001081"
     CDS             36239..37867
                     /locus_tag="ACGI59_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HFG9738885.1"
                     /translation="MNIIISLMGGLGLFLYGMNLMGEGLQKSAGTKLKKIIKLLTSNL
                     FMGVLVGTGVTAVIQSSSATTVMVVGFVNAGIMTLKQAIGVIMGANIGTTVTAQLVSF
                     DLTGMAPVALGVGIILYLFGNKPRIKNIAEILIGFGILFTGMDFMKMAVEPLRDYQGF
                     TDLLVTFGRYPLLGLLLGFGITAIIQSSSASMGMLVALAAEGLVPLSAALPILYGQNI
                     GTCVTSLLSSIGANKNARRAAMMHLIFNVLGTVIFLIFLNKPVVSMVTSWDPSNVARQ
                     IANTHTLFNIISVLILLPFTNLIIKLAIKLVPDRAGDIDEDETKTIKYIDDRMIETPS
                     IALANTIKEALRMGEKAKESLNASMEALVEHSTEKIDKTYRRERLINDLQKAILNYLL
                     KLSKAPLDDDSREVVDTLFNTVNDIERIGDHAKNIAELSQVAIDSNISFSEEGQSELD
                     VMYNRVVSAYTYALESMRTDNVDLACKVIKIEEQVDIMEKSCRANHMYRLNNNLCSIE
                     NGVIYLDVISNLERISDHAVNIAQQVIAKRLGND"
     gene            38132..39481
                     /locus_tag="ACGI59_001082"
     CDS             38132..39481
                     /locus_tag="ACGI59_001082"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFG9738886.1"
                     /translation="MGKINFDYSKATEFFCQNEIDVMQPYVDVAHDMLHNKTGLGNTF
                     LGWIDLPKNYDKEEFDRIKKSADKIKSDSDVLLVIGIGGSYLGSRAAIDMVSHSFRNG
                     LKKEQRKAPEVYFVGHNISSTYIMDLLDIIEGKDISVNVISKSGTTTEPALAFRIFKD
                     YLEKKYGKEEARKRIYATTDASKGALRQLATEEGYETFVIPDDVGGRFSVLTAVGLLP
                     IAAAGLDIDAMMKGANDAREAFQNPDLKSNDSYRYAVARTILHRKGKDVELLVNYEPQ
                     LHYVSEWWKQLYGESEGKENKGLFPASVDFSTDLHSMGQYIQDGKRLLFETVLNVENC
                     KRNITISSEEVDLDGLNYLAGKTVDFVNHKAFEGTLLAHTDGKVPNLVINIPQLDEYN
                     FGYLVYFFEKACGISGYLLGVNPFDQPGVEAYKKNMFALLGKPGYEKEKEELEKRLK"
     gene            39637..40716
                     /locus_tag="ACGI59_001083"
     CDS             39637..40716
                     /locus_tag="ACGI59_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HFG9738887.1"
                     /translation="MSRRKKGISLVILVAIISSILSSFLTIILVKDNLVSKSTGSSTP
                     IVVNDDGKSQNIYQAVAEKATPSVVGITTTSVDTSNMFAIPTETQGVGTGIIVDSNGY
                     ILTNSHVISDGQATSVNVLFNDGSTTSGKVVWFDQQLDLAIVKVDKTGLTPAEFADSD
                     KVKVGDISIAIGNPLGLDFQKTVTQGIISGLDRTIQTEKTNMTGLLQTDASINAGNSG
                     GPLLNQKGQVIGINTAKASQAEGLGFAIPINTAKSIVEEVIKNGKYEKVTLGIKGTDV
                     SNYEAATGTKLSTDKGVYVAEVISGSSAEKAGVKVGDIITKVGDTDITGMNDLNKKLY
                     TFSKGASTKITVNRGGKAVTINVNF"
BASE COUNT        16797 a         4308 c         7349 g        12412 t
ORIGIN      
        1 aataaatttt tatatttaag taaatacgtg taaaatgaat ataaagtttt tgtatctaag
       61 atattttatt tgtaaaatag taattaatta ttgtaagcta taatatgaga gtattatggt
      121 taatgtgaat tttaaagata ttattaaata tataaattag aatactatta aacggataaa
      181 tggaggagtt attatgtcta agatattagt agtagatgat gagttagata tgttaaagtt
      241 gattgaaaat gtgttgaaaa gagatggaca tgaagtaaag gtaattagtg atgtagaaaa
      301 agctatgaac atggatttta attattttaa tctaataata ttagatgtta tgatgcctaa
      361 gatggatggt tttgaagtat gtaaaactat aagaaataag gttgattgtc caatactgtt
      421 tttgacagca aaaaatatgg aaagtgatat tatgtacggt cttggtatag gtgcagatga
      481 ctatataacc aaaccttttg gtgtaggaga attaagggct aggataactg ctcatttaag
      541 gcgagaaagt agagaaaaaa agaatttatt aactatctca aatgttcaat ttaacttttt
      601 aggcaaagaa gtttcaattg atggtaaaaa aattaatttt actaaaagtg aatatctaat
      661 atgtgaattt ctagcaaaaa ataagggaca agtattttca aaagaaagaa tatatgagtc
      721 ggtatatggt tttgatggag aaagtgatat aagtactatc acagagcata taaaaaatat
      781 acgaagcaaa ttaaaacaat atgatattta tacaatagaa actgtatggg ggatagggta
      841 taaatgggtg taataagtat aagtgttgtt tagtgaaaat attatatata tctacaaaat
      901 gtttgtaaaa tattaatcta tatattaaaa tttaatttat agattaatat tttttgtaaa
      961 tgacaaaatt gtaaggtatt ttgttataaa atattaagat tcatagtgta tactaataat
     1021 atagtattta gtataaatct tgagcaaagg atggttattt ctaatggata gactaaagaa
     1081 tattagaaag agtttactac agtatacatt tttatttttg ttaataggta taacaattta
     1141 cctagtgttt aaaacattgg atattaaaat gctatcaaat gttatggtta tggtagataa
     1201 gaagttttta ttcataggtg ctttggcaat aatgtgtcac ataatgcttg aaggagtagt
     1261 aatgaagata attatagaaa gcactcacaa agttaatatt agatttattg gttttaagct
     1321 agccattatg ggattttatt ataatttaat aactcctttt gcttcaggaa gccaaccagt
     1381 acagatatat gtacttaaaa aatgtaaaat gccacttagt aaggcaagtg cagttgttac
     1441 caataagtct attatcttcc aaatagtagt tactttttat tgtacaatac ttgttttaat
     1501 gaattttgct atgttagaaa aacaaatgaa aatgattatg cctctagtgt ttctagggat
     1561 agccattaat tcatttgtct taattatgat aatattagtt attttaaatc ctgaaaaaat
     1621 aaaatatttt atgagaatta ctataaagca tttatcaaaa attaagtttt taaaattttt
     1681 agataataag attgaatcca tagaaagttt tatagatgat tatagtaaag caataaattt
     1741 ttttgcaaag aataaaaagg tattaatttc aactataata gtaactttta ttcaattaag
     1801 tgcttacttt agtgtttcat tttggatata taaagcattt aatttacaag gttatagcta
     1861 tatttatata ctgacattac aagcattttt atatatggca atatctccaa taccaacacc
     1921 tgggaatgta ggagctaatg aattagcctt tttcactata ttcaaatcag tattccctca
     1981 acctttgatg ggatatgcag tatttttata tggtggattt atgtactatt tgattctgat
     2041 tggaagtgga atatttactg taattactca ctatagaatg aaaaacagaa ttggcagaaa
     2101 tattgtttta agtgaaaact aaataagttt aaagaatata tattgagtat attaaaaaat
     2161 atgtcataag aactagaata ttttaattaa atggttgaaa tataaagatt attatgacat
     2221 attttttttg aaatttaaga ggaattgtga gaaaattgtt gaatttaata gatagtattg
     2281 caaaatattt atgattttgc atattataaa aacactcatt atctttaaga taaattttac
     2341 ttatcagggg ataaatctaa ggggggaatt gtatggagaa aaaaatttta acagtttgtc
     2401 catactgtgg agctggatgc caattatatc tagtagtaaa agataataag atattaagag
     2461 ctgaaccggc taatggtaga acaaatcaag aaagtttgtg tttaaaaggt cgttatggat
     2521 gggattttct aaatgaccct aaaattctaa cacctaggct aaaaaagcca atgataagaa
     2581 aacaaggtgt actagaagag gttgaatggg atgaggctat aaactataca gcttcaagac
     2641 taaatgaaat taaagaaaaa tatggacctg attctattat gggaacaggt tctgcaagag
     2701 gacctggtaa tgaagcaaac tatataatgc aaaaatttat gagagctaca ataggaacta
     2761 ataatgttga ccattgtgct cgtgtctgac atgcaccatc tgtagccggt ctggcttact
     2821 cattaggtag tggtgcaatg tctaattcta taccagagat agaaaatgca gatgttttat
     2881 ttatatttgg atataatgga gcagattcac atccaatagt ggcaaataga atagtaaaag
     2941 ctaaaaaaaa tggtgctaaa ttaattgtta ctgaccctag agtaactgaa tctgcaagaa
     3001 ttgctgatat acatctacct ataaaaggtg gaacaaacat ggttttagta aatgcttttg
     3061 gaaatgtatt aatagaagaa ggtctttaca ataaagaatt tgttcaaaat catacacaag
     3121 gatttgatga gtataaagaa attgttaaac catatacagc taaatatgct gaaaagatta
     3181 caggaatccc agaagagctt ataagaaaag ctatgagaga atatgcaaaa ggtaaaaaag
     3241 ctatgatact ttatggaatg ggtgtctgtc aatttggtca agctgttgat gtagttaagg
     3301 gacttgcatc aattgctcta cttacaggta attttggtag agaaagcgtt ggaataggac
     3361 ctgtacgtgg acaaaataat gttcaaggag cttgtgatat gggagctctt cctaatgtat
     3421 atcctggcta tcaaaatgta actgatgata agataagaga aaagtttgaa aaagcttggg
     3481 gggtaaaatt atctcctaat aatggttata gtttaactca agttccaaat ttagtactaa
     3541 aagagaaaaa gcttaaagct tattatatat ttggagaaga cccagtgcaa agtgacccag
     3601 atgcatcaga agttagagaa gcattagatg aattggaatt tgtcattgtt caagatatat
     3661 ttatgaataa gacagcactt catgctgatg ttatacttcc agctacttct tggggagaac
     3721 atgagggagt atatacatgt gcagatagag gattccaatt gatgagaaaa gctatagagc
     3781 cacaaggaga tgtaaaacca gactggcaaa ttataagcga aatttctaca gcaatgggat
     3841 atcctatgaa ttataaaaat actaaggaaa tatgggacga acttagacaa ttatgtccaa
     3901 gtttcttagg tgctacttat gaaaagatag aaactcaagg atgcgttcaa tggccttgta
     3961 agagtgaaag tatggaagat aaaggaacta tgtatctgta tgaaggtcaa aagttttcta
     4021 ctcctaatgg taaaggtaat ttgttcgctg ctgaatggag accccctatg gaagttgaag
     4081 atgatgaata cccatttagt ttatgtactg taagagaagt tggacactat tcagtaagaa
     4141 caatgactgg taattgtaga acacttagtt cattagaaga tgaaccagga cgtgttcaaa
     4201 taaattctaa tgatgctgaa aaattaggaa ttgaagatga tgaattagtt agaattagtt
     4261 caagaagagg aagtgtaata acaagagcaa cagttactga tagagttaag gaaggtgcaa
     4321 catatatgac ttatcagtgg tgggttggag cttgcaatga acttacaata gcaaatttag
     4381 accctatatc taaaacacca gagtataaat attgtgctgt taaacttgaa aaacttgaag
     4441 accaagaatt agcagaaaaa tgtgtaagag aagagtatca aagtttgaaa gacaaaatga
     4501 cagctacaaa tatctaatga tttattagat ttaagattta taaaaggcag aaaggctaat
     4561 attttatgaa caatatatac tcaaaaacta aattacaaga aaatagtaag gataaagttt
     4621 ttgaaaatag ttatagtcta gtggactaca aatatttaaa ttcttttaat gtagaagtaa
     4681 gcaaaataaa tgaacatgaa gtatcaatag ataatgaaga aataattgca gaatatccac
     4741 ttagttttat tttaaatgat aaatatgtaa atacattttt atgtacccca tataatttag
     4801 aggaacttat agtaggattc ttgaaaacaa aaggatacat agaaaacaaa aaaagcttat
     4861 taaaatttga gataaatgag agtgaacgtt ttgcaaaagt aactattcaa gagaaacaaa
     4921 aaaatcaaga tgagcaaatt atatttttaa attcactaga ctatataaca tgtgctcctg
     4981 taaagagcaa tataaaaata aattatgata cgatttacag tataatggat aaaaatttaa
     5041 attcatcaga attatttaaa aatactggtg gggtacatag cgtatctgta tttaattttg
     5101 ataaaaaaat agtcacttgt gaagatgtag caaggcataa tgcaatggat aaggctattg
     5161 gattttgtgt tttaaatgac ataacattaa aagataaaat tgtggctgta agtggaagaa
     5221 tatcattgga aatgatatta aaagcagcta tgactcaaat tcctattgta gtatcaaaat
     5281 cagcaccgac taatttatca atagagcttt ctaaaaaatt aaatataacg cttattggat
     5341 ttgtaagagg aagacggatg aatatttatg caaatccaca gagagttatc aaaaagtgag
     5401 aatgaatata tgtgtactaa aaaagaagag caaagatatt caaagtgtga aatctgtgga
     5461 gtattaattg atgatactga taaggtgtat aataacacat acaatttgtg caaaaaatgt
     5521 aaacgaaata taagtactga gaaaatagtg aaatatataa atatatatga tgaaagtaag
     5581 tagacatatg atgaaaataa ctagagggtt ggaaatatac aaattgaaag gtgggataat
     5641 atgaaaaata aaataggctc ttttgtaata gcagacccaa acaaatgtac aggttgtaga
     5701 gcttgtgaag tagcttgttt tacaatgcat aatcagcata ataatgtggg ttatacagtt
     5761 ggaacagttg atatacctgt tatacctaga ctttacttag taaaaggtga caatttctgt
     5821 atgccaatac agtgtagaca ttgtgaagat gctccttgtt taaactcatg tccacaaaaa
     5881 gctatagtta aagaaaacaa tattatgtct gtaaatgaag aaaagtgtat aggatgtaaa
     5941 acttgtctgc tagcatgtcc ttttggagct atagacttac ttccacaata tgaagatggt
     6001 aaagaagtag aacaagtaat tttagatgaa aataagaaga tagcttataa atgtgactta
     6061 tgtaaagaca atgaaaaaat agcttgtata agtgcatgtc ctcaggaagc attaaaactt
     6121 gttactccaa tagatgacaa aaaagctaag aatagacaag ctgcacttag tttattgtta
     6181 acaaataaat aaaggataca ggggtaggtg acgtttatga taataaatat agataaggat
     6241 ttatgtactg gatgtagaga atgttcaaag gtctgtccag taaatgctat tgaaggtgaa
     6301 gagggaaagc cacaagagat aaatttagat agatgtgtta tgtgcggtca atgtgttcaa
     6361 acttgtaaat cttatgcctc agttatagat gaaggatttg agtttttaca agagaaaaaa
     6421 caagaaagag agataccaga gtctataaat gaacctatat ttgcagcata caatgtatgt
     6481 gatatagata aggtgaagaa agctttaagt gacccaaatg tattcactat ggtccaatgt
     6541 gcaccagcag ttagagttgc tttaggagaa gattttggat attatttagg atacttaggt
     6601 gctgggaaaa tggcagcagc acttagagct ttaaattttg atagagtcta cgatactaat
     6661 tttgcagcag atttaactat aatggaagaa ggtagtgaat taattaaaag agtgacagaa
     6721 ggtggaactc ttccaatgtt tacatcttgt tgtccagctt gggtaaaatt catggaaaaa
     6781 aattatccta aattaactaa tcatttatct tcttgtaaat cacctcagca gatgggtggt
     6841 gcaatattta aaacttatgg tgcagagata aataatgtag atgcatcaaa gatatttagt
     6901 gtttctataa tgccatgtac atgtaaaaaa tatgaatgtg atagagaaga aatggattct
     6961 agtggatata gagatgtaga tgtagtatta actactagag aactagctta tttaataaaa
     7021 gacatgggaa ttgattttaa aaatttaaga gaagaaaaat ttgatagtcc tttaggtagt
     7081 tatagtggag ctggaactat ctttggtgtt actggtgggg ttatggaagc tgctataaga
     7141 acaggatatg agcttataac tggagaaagt attcctgatg tagaagtaaa acaagtaaga
     7201 ggagaaaatg gttttagaaa gtcaactatt aaaataggag acttagactt aagagttgga
     7261 gtagtaagtg gattaaaaaa tgtaatacca gttcttgaag acttggaaaa aggaaaatta
     7321 gatgtagatt ttattgaagt tatgacttgt cctgttgggt gtgtaagtgg tggaggtcaa
     7381 ccaaaaattt tactagaaga atataaggaa ttggcttatg aaaatagaac aaaagcaaca
     7441 tacgtgcatg atgaacattt agcaattaga aaatctcatg agaatcctga tattaaaaaa
     7501 ctgtatgatg aatatctggt agaaccatta ggagaaaaat ctcatcatct attacacact
     7561 acatattgta taggaaagga agtggctaag tgatgaacta ttttgttata gcagaccctg
     7621 ataaatgcat aggatgtaga acttgtatga taggatgtgt agttgcacat tcagatgaag
     7681 atatttttta ccaaaatcca gatgaaataa attttaatcc aaaactttct gtcataaaga
     7741 caaaagaagt aagtgctcca atacagtgta gacattgtga agatgctcct tgtgcaaagg
     7801 catgtccaaa tggtggtata gttagggttg gtaacacaat aaaaataaat gaagaaaatt
     7861 gtataggatg taagacttgt atgttagcat gtcctatagg tgcaatagac atagttacat
     7921 taaaagatgt agatgaagga aagctttgct ttagagaaag aatgacagca aataagtgtg
     7981 atttttgtat agaatcgcca gaagggcctg catgtgtaaa tgtttgtcca actaaggcat
     8041 ttactatagt taaggatgag gatatagata gtaaagttaa aaataagaga aaattagcta
     8101 tattgtaata taaaaaagat ggagttttat ttatgattaa aagctccatc ttttttatta
     8161 taatatttat atatctaaaa atcaattatt gcattagttt atctaatctt tctttattca
     8221 attttttcat tcctatagaa gttataataa gtaaagtata ggcaattata actgataaaa
     8281 ttataataga ccaagaaaat ctgtatccaa aattatcaac taacaatcca aataatgttg
     8341 aacctaatgc aaatcccact gcaaaaatta attgtacgat tccatatatg ctaccaaact
     8401 ccttttgacc aaagaaagag ccagttaaat aagcaggacc aatcatataa gcaaatacag
     8461 aaagaccttt caaaatggca aatacaaatg caaaagtaga agatttatct accaatagta
     8521 atgataaagt tgctataaga accaatacaa aagatataat aagtggcttt gtttgaccga
     8581 atttatcaaa caataatcct ccaaataaat taccaatgag agcaaataag gcaaatgtgg
     8641 agcctactgt tcctattaga ataggattta gataatttct aagataagtt ggatattgta
     8701 cagataatga tgaaacatat ataccaacaa atataaatcc aatagtcata atccaaaaat
     8761 atttaatttt tttagcttct tttaatgtat aaccccatac tgaaggagaa attgtatcag
     8821 atgctttctc ttttttacct ttagatttta ccttttcatt aatatcttta ggcatcctga
     8881 gtatgaataa tattacaggt ataccaacta ttaatgctat aattccaaat attagataag
     8941 aacgactgta tccttgagtt gctaataagc gaactgcaag tggttgtaga aatatactac
     9001 caatagaacc accagcaaaa gcaaagccca aagcttttcc tttgctttct tcatcaaacc
     9061 aagcacttat taaaacaggt attcctatag cagatataat tgtagcgcca atttgaacaa
     9121 tacatgatac agtgtaaaat tgccatagtt cagtacatat agaaaaagta gcaaaagcaa
     9181 tactagaaat tatacaacct aataaatata gtatcttaat attgacttta gaaaataata
     9241 cacctataaa aggtgatgca attgctgagg caatagtacc aatagtgaat attagagaaa
     9301 aagaagctaa actaaagcct tctccttcta caacataact tataaattga ggctgtatac
     9361 ttgctacaat actaaatgga acagcttgta ttaacataca accaagtact ataacccaag
     9421 catttgaaaa gtttttaatg ctaaatttat tgtttagtaa tgtattcatg gtatctctcc
     9481 tttaggttat gatttgtaga tattaatcta attattctaa ttttatatat tagttttgga
     9541 tagcctaact atttaaatat aatttgggta ttaatataaa aattaactat aaaaattgat
     9601 tattttaaat gtatggggta aattaaagta tataaataaa tgaaaggatg gataaaatgt
     9661 catttgaaaa aataaataat aataacctag ataaaataca caataggaag tgtattatgc
     9721 ttgtaaactt taataaaaaa gaattatcat taattaaaaa tgtaagtaac tttgctggaa
     9781 taagagacca tatcatctta agtgataaaa atggagagaa tataataaga gacattttag
     9841 atggaaatat atcaaatgaa tgtgaaaatg ggattaagag taagtctgta atatttaata
     9901 acactccaag tacacaagta aatgcatttt tagaagggct aaaaaaagtg agaataaata
     9961 gaccttttac agctatggta acagagacat ctattgattg gacattgaat aatttaatag
    10021 aaaatttagt tcaagaacgt aatgctatgt ctttaggaaa aacaattgaa cataagtaaa
    10081 tttaagatat cttatagtaa ttttaactaa tgtacattga gtatcatatt atattttaga
    10141 atagaaaatt ttaaatgtgg gtagatggag gagatttgag atgaaagttt tatttacaat
    10201 aaattatgga aaagaaaaat ttgataaaat aagagaatta ggctatgatg tcatacatta
    10261 ctctgaagat agtattgaaa ataatgagga tgtaaataca gcagatgtac tagttacata
    10321 caatccattt gagagattag atatttctaa gatgaaaaat ttacaatata tacagacaac
    10381 tagtgtaggt atagaccaac ttccaaaaga agaggtattg aagagaaata ttaagatagc
    10441 taataataga ggcggatata gtgtacctat tggtgaaagt attgtattgt atattttaga
    10501 aatatataaa aatagtgcaa aattttttaa acaacaacaa aataaagaat ggaagatgga
    10561 tttttctgta tcagaattgt atggacaaag agttggattt ttaggaacag gtactatatc
    10621 ttcagaagca gcaaaaagat taaaagcttt tggtgtagaa atatggggtg tcaatactga
    10681 tggaagtagt aaaaaatact ttgataagtg ttttgctact gaggatatga atattgtatt
    10741 tgaagaatgt gatatagtag ttgtagcaat gccttctaca aaaagtacag atggaatagt
    10801 aaacaaagat atgtttaaac ttatgaaaga tggttctgta tttataaatg taggtagagg
    10861 aaatacagtt aatcagaaag atttagaaga atgtgtagag aaatttagag gggtagcatt
    10921 agacgtattt gaaagtgaac ctttaaacaa ggacaataag ttgtgggagt gcgaaaatgt
    10981 catagtaact cctcataatt cttgggtgtc agataaaaac aaagagagaa catttaatat
    11041 ggtttataat aatttgaaac actatataat ggagaataaa cctgtagata atgttgtaga
    11101 catatcaaga ggctattaaa aataaaaaat tttgataaaa ataaaaactc ttattagtcg
    11161 tattaaagat actaataaga gttttttttg taataaattt aaagcccaaa aagtgatata
    11221 ctaaaaaata tataaatagg agataattaa ttatagggaa atttcaaaat aatttaattt
    11281 taaaaatatg aagaggtgca aaaagtgagc gtaaaagatg aaattaataa acttatagag
    11341 acaataaact accataatga aaggtattat aatcaagata gcccagaaat atcagattat
    11401 gaatacgata agttaatgaa ggagttaatt tctttagaag aagaacatcc agaactaaag
    11461 agaattgatt ctccaacaaa tagggtagga ggtaagccat tagataagtt caatcaaatt
    11521 gtacataaaa ctcctatgtt gagtttatca aatgcttttt cagagcaaga tttgagagat
    11581 tttgataaga gagttcaaga atatgtggga gaaaatgttg aatatgttgt tgaatttaaa
    11641 atagatggtc tttctgtagg tctaacttat aacaatggag agtttgaaaa aggagcaact
    11701 agaggagatg gagttgtagg agaagatatt tctcaaaact taatgacagt aaaaagcata
    11761 cctcttaaga taaatgataa aaaagaacta atagttagag gagaagtata tatatcaaaa
    11821 gataatttta gaaaagtaaa tgaacaacaa gaagaacaag aacagccatt atttgctaat
    11881 ccaaggaatc ttgcagctgg ttcattgaga caattagacc caaaactaac agcaaagaga
    11941 ccattagata tatttgtatt taatatggaa aatatagaag aatacgattt agaaagtcac
    12001 agtgaatcac tagagtactt agaaaaacta ggattttcag taagtcagag ttataaagtt
    12061 tgtaaaagta tagatgaagt tattgagcat attgaatact ggacagataa caggggtaat
    12121 ttagactacg aaatagatgg tatggtaata aaagtaaaca atataaaaca aagagacgaa
    12181 atgggatata cagccaaaag tcctagatgg gcaattgcat ataaattccc agcagaaaag
    12241 aagaaaacta aaatagtaga tataatagta gaagttggga gaactggtac aataacacct
    12301 tcagcaattc ttgaaccagt cagacttgca ggtaccactg taagtagagc tacacttcac
    12361 aatgaagatt atataagaga aaaagatata aaaataaatg atacagtatt agttcaaaaa
    12421 gcaggggaca taatacctca agttttagaa gttataaaag aagaacgtac tggagatgaa
    12481 atagatttta aaatgccaga aaagtgtcca gtttgttcag agccaacaat aagattagaa
    12541 ggagaagctg ctgtaaaatg tatcaatatg tcttgcccag cccaaataag aaggggaatt
    12601 atacactttg tgtcaaggga tgcaatgaat atagatggat tgggagaatc tataattaca
    12661 ctgctattaa atgaaaaaat tattcaagat gtatctgatt tatattatat aaaaaaggaa
    12721 gatgtagtcg acctagagag aatgggcgaa aaatctgctg acaatttaat aaattcaatt
    12781 gaaaaatcaa aagaaaatga tttatggaga cttataaatg gattaggtat taaatttata
    12841 ggagttaaag cagctaagat acttgcatat aactttaagg atttagatga agtaatttct
    12901 gcaactagtg agcaactaga agaattagag gaatttggaa gtataatggc aaacagcgtt
    12961 gtcgagtttt ttaaggaaga caaaaatatg aacgttataa acaagttaaa aaatgtagga
    13021 gtaaatacaa aatcattgga taatacaggt gaaaaagtag aaaaaatatt tgaaggtatg
    13081 aaaatcgtat tgactggtac acttcctaca ttaaaaagaa atgatgcaaa agatatgata
    13141 gaaaaaagag gtggaaaagc tacatctagt gttagtaaat cgacaacatt tgttttggca
    13201 ggtgaagaag ctggttctaa acttactaaa gcaaatgatt taggaattaa agttatagat
    13261 gaagagagat ttttagaatt attaaagtta tcatcgaaag atgaagtaaa agctgtatta
    13321 gagagttaaa aagtcgctta atttagtaga aaactactat aattaatgat agaatagttc
    13381 agtaagctaa taagattacg aaaaatgaca ataattaata tttgacaaaa ttaaaaatta
    13441 taaaaataaa tttataaaaa ataaaaaata tgagaaagga tataaaaaat gaccagaagt
    13501 tatgatagaa gtaatgaaca gataagacct gtaaagataa ctagagatta tacaaagtat
    13561 gcagaaggtt ctgttcttat agaaatggga gagacaaagg ttatatgcac tgcatcagtg
    13621 gaagaaaaag ttcctccttt tttaagaaat agtggtactg gatggataaa tgctgaatat
    13681 tctatgctac caagagcaac acaacaaaga aagataagag atgcatctag gggaaaaatt
    13741 gatggaagaa cacaagaaat acagaggtta ataggaagaa caataaggtc tgttgtagac
    13801 ttgaataaat taggagaaag aactatttgg gtagattgtg atgtgattca ggcagatggt
    13861 ggaactagaa ctgcatctat aacaggggca tttgtagcag ttgcagaagc aatatataaa
    13921 ttatataaag caaagacaat aaaagagatg ccaataacaa attttgtgtc tgcaattagt
    13981 gttggtattg taaatggaat acacatgcta gatttatgtt atgaagaaga ttctaaagca
    14041 caggtagata tgaatataat aatgactgat aagtgtgagt ttgtagaagt acaggggaca
    14101 ggagaagaga aacctttttc tagagcagat ttaaatttat tgttagagtt aggtgaaaaa
    14161 gggaataaag aattaataag gcttcaaaga gaagctttag gtgaaatatc agatgagata
    14221 ttgggtatgg attatggtaa tgaagtagtg attgctacta ataatgctca taagctagaa
    14281 gaaataggtg aaatcttaaa agattttgaa tataaagtgt actctttaaa agatgttgat
    14341 ttagcaggta tagagatagt agaagatgga aaaacatttg agcataatgc gttgataaaa
    14401 gctagaacaa tagcaaaaaa gactaaactt atagcaatat cagatgattc tggcttagaa
    14461 gtagatgctt taggcaaaaa acctggtgtt tattcagcta gatatgcagg agaacatgca
    14521 acagatgaag aaaatagaaa aaaactgtta aaagctatgc aaaatgtacc aatgagcaaa
    14581 agaaatgcaa gattcgtctc tgcaatagca gtagtttttc ctgatggaaa agaatttgta
    14641 gtaagaggaa tttgtgaagg aatgatagga tttgaagaaa aaggtaaaaa tggttttgga
    14701 tatgatagct tatttatagt aaaaggatat gataagactt ttggtgaaat acctagtgtt
    14761 ataaaaaatt caatcagtca tagagcaaac gcactaaaac ttatgaaaca agagtttata
    14821 aaaagggtag tgaagtaatg aaaataggca tagtaagcga cacacatatg atgattaaaa
    14881 atatggaaaa aacaatacca tatttgaaag aatgtgacct aataattcat gcaggagata
    14941 actttactga ttcaagatat attcatagca tgactaatgt aggtattatt gcagttaaag
    15001 gtaattgtga ctttgatgct gttgaggaag aagttgtctt tgaagtggca aataaaacca
    15061 tattcttatg ccatggagat aagtatggtg taaaatatgg aactaatatg ttagaaaaaa
    15121 aggcaactga agttgatgct gatatagtta tatttggcca tacccatacc ccatttagag
    15181 aaattaaaga tggtgtactt tacataaatc ctggaagcac ttcacttcca agaggagtat
    15241 cgtataaaag ttttgtcatt atggatatag aagaagatga cataaaaatt gaagaaatac
    15301 gcatataaag gtgtatttat ctaaaaaaat ggcaaaaaac gctcaaaaaa ttaaatttta
    15361 tcaaataagt acaacgatat taatggaaca cttgtaaata aaaaaactta tgtgatataa
    15421 ttaagtggtt gaattataaa tagaatgaat tataataccg ttatcgggta ttacataaat
    15481 atcactagga ggattagaag gatgaaagca gaactaatta aaaaagaggg taataaagtt
    15541 actttaaaaa taacagtaga taacaataaa tttgaagcag cagtaaataa ggcttataat
    15601 aaaagcagaa ataaatacaa tatacctgga tttagaaaag gtaaagctcc aagaattgtt
    15661 atagagactc aatacggaaa aggtatattc tacaatgatg ctatagaaat attattccca
    15721 gaagtttatc cagaagctat caaggaatta gacatagacc caatagacaa tccagattta
    15781 gatatagaag aaataagtaa agacaatggg ttggttatgg ttcttaatgt agaagttaaa
    15841 ccagagtttg aattaggaaa ctacaagggt atagaaatag ctaaggttga gaatactgta
    15901 agtgacgaaa atgttgatgc taaactacaa gaaatggttg aaaaaaatgc cagattagta
    15961 agtgtagaag acaaagcttt agaagatgga gatacagcta taatagactt cgaaggtttt
    16021 gaaaatggtg tagcttttga tggtggtaaa ggtgaaaatt ataacttagt tataggttca
    16081 aacacattta taccaggatt tgaagaacaa ttagtaggta aaaaagctgg tgaagaagta
    16141 gaagttaatg ttactttccc agaagaatat cactcacaag atttagctgg aaaaccagtt
    16201 gtatttaatg taaaaataaa tgatgtaaaa gtaaaagaat tatctgcact tgatgatgaa
    16261 tttgctaaag atactagtga atttgattct ttagatgaat taaaagcaga tgtaagagct
    16321 aagttagaag aagaagctaa aaatagagca gatgcagaaa ctagaaattc agtagtagaa
    16381 aaagttgctg aaaatacaga aatagaaata ccagaagtta tgatacaaca tcaaatagat
    16441 aatatgttaa atgaacttaa ctaccaatta caatatcaag gatttggatt acaacaatta
    16501 ctagagatga ctggtagaac tatggaagaa ttaagagaag agagaaaaga agatgctaaa
    16561 aaattagtta agtcttcttt agtattagaa gcaataacta aagctgaagg aattgaagca
    16621 acagaagaag aatttaaggc tgagttagaa aaaatggctt ctgcttacaa tatggaagta
    16681 gaaaaaatag aagcttcttt aagagatgct gacaaagaag atataaaagg tcaaataaaa
    16741 ataagaaaaa caatagattt attagttgat aatgcaacaa tagcttaata aaataaacgc
    16801 taactaatat agttgacgtt atgaaatgtt tagtatataa tgatgtgcaa atataattta
    16861 gttaaatgcg tataataatt tacagtagct taaaaaaagc tactgtaaat tatatagaat
    16921 aaagggggta tgaaaccatg gcattagtac ctgtagttgt tgaacaaaca ggaagaggag
    16981 aaagatctta tgatattttt tctagactat taaaagacag aataatcttc ttaggagatc
    17041 aagttaacga tgccacagca gggcttatag tagcacagct attattttta gaagctgaag
    17101 acccagacaa agatatacat ttatatataa attctccagg aggaagtata acttctggaa
    17161 tggctatata tgatactatg caatacatca aaccagatgt aagtactata tgtataggta
    17221 tggctgcttc tatgggagca ttcttgttgg cagcaggagc aaaaggaaaa agactagcac
    17281 taccaaatag tgaaataatg atacatcaac cattaggtgg agctcaaggt caagcaacag
    17341 atatagaaat tcatgcaaag agaattttaa aaataaaaga aactttaaat gagatattat
    17401 ctgaaagaac tggtcagcca ttagaaaaaa ttaaaatgga tactgaacgt gataacttca
    17461 tgagtgcact agaagccaaa gaatatggtt taatagatga agtatttaca aagagaccgt
    17521 agatagagag aggtgttaat atgtctaaat atgaagaaaa acgacaatta aagtgttcat
    17581 tctgcggtaa aaatcaagat caagtaagaa gattaattgc aggtccaaat gtatatattt
    17641 gtgacgagtg tgttgaactt tgtgacgaaa ttattcagga agaaatagaa gatactatag
    17701 atgaagatac tacatcttta ccaaaaccaa aggaaatgat ggagattcta aatgattatg
    17761 taataggtca agaaaaagct aaaaaagctt tatcagttgc ggtttataat cattataaga
    17821 gaatatacag taaaaaatct agtagtaagg atatagaaat tcaaaagagt aatatacttt
    17881 tattaggacc tacaggttct ggaaagacat tacttgctca aactctagca agaactctaa
    17941 atgtaccttt tgcaatggca gatgctacat cattgactga agctggatat gttggagaag
    18001 acgttgaaaa tatactttta aaacttattc aagctgctga ttttgatata gaaaaagctg
    18061 aaagaggtat aatatatata gatgagatag ataagattgc aagaaaatct gaaaacccat
    18121 ctataactag agatgttagt ggtgaaggtg ttcaacaagc acttctaaaa atattagaag
    18181 gtactgttgc aaatgttcca ccacaaggag gaagaaaaca tcctcatcaa gaatttttaa
    18241 agatagatac tactaatgta ttatttatat taggtggagc ttttgatgga ttagagaaga
    18301 taattcaaaa acgtggtgga gataaaacac taggttttgg agctaaaata gaaagtaaaa
    18361 aagaattaga tttagggaag ctgtatgaaa aagtacttcc agaggattta ttaaaatatg
    18421 gtataatacc agagtttata ggaagaatac cagttttagc tactttagag ttgttggatg
    18481 aagatgcatt aatgcagata ttacaggaac ctaaaaatgc tctagtaaaa cagtataaaa
    18541 agttattgga gttagatgat gttgagctag aatttgaaga aggtgctctt cgtgcaatcg
    18601 ctaaaaaggc catagaaaga aatacaggag ctagaggact tagaagtata gttgaaagcg
    18661 taatgatgga aactatgttt gaagtaccat ctagagataa tataaagaag gttatagtaa
    18721 cagagaaatc tgtgaacgaa gactcagtaa atcctatcat agtacttaag gatcaagagg
    18781 aaagtgcata agatgatgaa aaggagcttt aaaagctcct ttttttatgt taatatgcta
    18841 aatataaaat aggtatcatt attataaaaa tgtgagtaat atacctagaa taccaaagac
    18901 gaatacaatt gcaatggaac caatttttcc tttatataaa agataaaatg atgctataaa
    18961 tgcaattatt ccatataaat cccaattaac ttctttggca gatgaaaaag ctgctgataa
    19021 tatgaatcct agagtaattg gtctaagaac tgtcaataag ttttctacct ttttattatt
    19081 tttaaattta gaaaataact ttgatataat agttagacct attaatgcaa aaattgtaac
    19141 accaatagtt gcagctagag aaccaaagaa gccagcagtc ttaaatccta caaatgtagc
    19201 actatttatt gctacaggac ctggtgtaga ttgtgatata gcaagtaaat ccaaaaactc
    19261 ttgattagtt ataaagtgat atttagatat aaattcttgc tggataaatg gtagcatggc
    19321 ataaccgcca ccaaaactaa atgcccctat tttaaggaat attaagaaga gtagtattat
    19381 ttctgacata gactatatac acctcctcca attattaaat aaatagggct tatattgaat
    19441 acaccaacag ataaaactgc tataattaat agtagtatgt tagttttaga ctttggtagt
    19501 ttcttgaaca tgctcgtaaa agaatacaat ataagagcta ttacaacagg cgcaactcct
    19561 ttaaaaaatc catctaaaat tttagatgat gaatttttaa agtatagata cgataagact
    19621 aagactatac aaaatgatgg taaagcagag cctaaggtac atataagaac tcctggtata
    19681 ccataaagtt tatatcctaa taatacagat atattaactg ccagtactcc aggataactt
    19741 tgagctatgg ataaataatc aataaattct tcctgctcta aaagtttttg tttgtttaca
    19801 agttcatctt gtataagagg aaccatagcg tatccaccac caaatgtaaa cgcaccaatt
    19861 tttaaaaagg ttaaaaacaa tttcaacata atagaaacct ccaaaagata aataacatct
    19921 aagtatacta atattaatat tatatcataa taaaaagata atatagtatg atagtattct
    19981 aagaatttat cattaatgtt gaaaattaac ttgttcaaat atataatatt aaaagcaaag
    20041 caattagact agaaaaggag taatgttgat ggaacaaaat tatactaaaa tagatcatga
    20101 attaccatta attcctctaa gaggattagc tatatttcca tatatgatat taaattttga
    20161 tataggtaga gagatatctc ttaaagcttt agaccaagct atgatggatg aagaacttat
    20221 atttttaact tctcagaaag aagcagaggt agatgagcca ggagaagagg atttttatca
    20281 tgtgggaaca atctgtaaag ttaagcaaat gataaaatta ccaggagata cagttcgtgt
    20341 tttggttgaa ggtgtatcaa ggggtagagt aaagaaaata gaacaagaag atgggtattt
    20401 cagagcagta atagaagaaa tagtatttga tagtgataat ttggatagtg aaacagaagt
    20461 tgaaattgaa gcatttgtaa gaaatgtatt tgatgccttt gaagagtata taaatattgg
    20521 aaatagggta tcaccagaaa tattaatatc tttagctgat atagaagatg tagatagatt
    20581 tatagatacg atagctgcaa acatatatct aaaatcaagc caaaaacaag aaatattaga
    20641 agaatttgat attagaaaaa gacttgaact tatttattct atattattag aggaaataga
    20701 tatattaaaa atagaaaaga aaataacttt aagagtaaaa aaacagatga ataaggttca
    20761 aaaagaatat tatcttagag aacaattaaa agctatacag aaagaattag gtgaagaaga
    20821 agatataaac tctgaagcag atgagtatag agaaaagctt aaaaaaataa aagcacctaa
    20881 gacaactaaa gagaaaatag aaaaagagat agataaattt tcaaaaatat cttctatgtc
    20941 tccagatgta tctgtaagta gaaactattt agatactata ttctcattgc catggaataa
    21001 agagacaaaa gataaattag atataactaa ggcaaaagat atattagatg aagaccatta
    21061 tggattagaa aaagttaagg agagaatact agagtatcta gctataagaa ctcttgctaa
    21121 gtcacttaaa gggcctataa tatgtctagt tggtcctcca ggaacaggaa agacatctat
    21181 agtaaaatca atcgccagag cattaaatag aaaatttgta agaatatctt tgggtggagt
    21241 tagagatgaa gcagaaataa gaggtcatag aagaacttat gtaggttcaa ttccaggaag
    21301 aataattaat ggggtaaaag aagcccaaac aaaaaatcct gtattcttat ttgatgagat
    21361 agataagatg gcagcagatt ataaaggtga cccagcttca gcaatgctag aagtattaga
    21421 cccagagcaa aataaggatt ttgtggacca ttacttagaa ataccttttg atttgtcaaa
    21481 aatactattt gttacaacag caaatagtct aagtaatata ccaagaccat tattagatag
    21541 aatggaagta atagaagtat ctggatatat agaagaagaa aaattaaata tagcaaaaaa
    21601 atacctactt ccaaaacaaa ttaaagaaca tgctttaaaa gaaaacttta taaaaataga
    21661 tgatgaaact ttaagaagta taatcaacca ttatacaaga gaagctggtg taagaacact
    21721 agaaagaact attggtaaga tttgtagaaa ggttgccaaa aaatacgttg aagacccaac
    21781 tcttgaagag gttgttataa ataagtctga cctagagaca tatcttggaa aagacatgtt
    21841 taagtatcaa cttgcagaag ttaaccctca aataggatta gtaaatggtc ttgcatggac
    21901 agaagttggt ggagttacac ttgaagtaga agtaaacgta cttaaaggta aaggagaaat
    21961 tgtattaact ggaaaattag gagatgttat gaaggaatct gcgaagacag gtatctctta
    22021 tataaggtca atagtggaca agtttgatat agaccctgaa ttttataaga cgaatgatat
    22081 tcatatacac attccagaag gtgctgttcc aaaagatgga ccatcagctg gtataactat
    22141 ggctcttgca gtaatctcag cacttactaa aagacctgtg cctggaaata tagcaatgac
    22201 aggagagata actttaagag gtagagtctt agcagtagga ggagttaaag aaaagttatt
    22261 agcagctcat agagctggta taacaaaagt attaattcct aaagaatgtg aagctgattt
    22321 agatgaaata ccagaaaatg taaaagaaaa aatggagttt gtgcttgtag aacatatgga
    22381 tgaagtatta gaacaagctt tattaaagag tggtgaaaat aatgaaaatt agaagttcag
    22441 agataactat gagtgctgtg aataaatcac agtatccagc agaaggaatc ccagagatag
    22501 cattggctgg taggtcaaat gttggaaaat catcaataat aaatacattg cttaacagaa
    22561 gaaacttcgc tagaaccagt caaacaccag gaaaaactag aactataaat ttttatctaa
    22621 taaataatga attttatttt gtagaccttc caggctatgg atatgcaaag atagctaagt
    22681 ctgaaaaaga aaagtgggga ggtataatgg aacgatatct agaaagtaga caagagttat
    22741 gttctatatt cctacttgta gatattagac atgagcctac agcagatgat aagcttatgt
    22801 atgaatggat aaagcacttt ggatataact gtgtagttat tgcaactaag gcagataaaa
    22861 tatctagagg acaatatcaa aaacacataa gtataataag aaaaaaattg caaatggaaa
    22921 gtagtgaaaa agtaattcct gtttcttcac taaaaaagac tggtgttgaa gaattatggg
    22981 aagagattgt aaatcaatac aatcaacatg gatatgaaat aacagtagat taaatttaaa
    23041 atgttaaata ttgacatgag atttatttga ataataaatg taataatagt tagttttaat
    23101 tatattaata ttaaagcaaa atataggatg ccttaaaata gttttaatga actttttttg
    23161 gcatccttat tgtttataaa caataaatat cagtaatttt gttaaatact aataaatttt
    23221 agtaaggtat gggaaaatta gtataaatta gttcaactaa aaaattattg acttatgtag
    23281 tcagcaataa atagcattta agaaatagat ttatctgttt ttctcataaa gaaaacagtt
    23341 agtataagcc ctataaaaca tactgatgct gcaatccaat aagactgatt gctagctata
    23401 acataggctg atgtgtgact tttaaacttt tgattgtatg taagactttg tgcaaaaaaa
    23461 ctactaaaaa atgctgttcc aagagcaaat gataaattac gaatagttgc aagaaaactg
    23521 cctccgtatc cctgtttttg aggaggaaca tctgctaaaa tactactatt gtttggtgaa
    23581 ctaaatgctc ctagcccaag tccaaaaata actaaacata caattaaaag atacaatgga
    23641 gaatctgctt ttaaaagtgc cattccaact agtgagatgg tcataaatga aaaagatgct
    23701 gttagaatgt tttttgcacc gtgtttatca gaaagtgaac ctgcaatagg tgctataagt
    23761 accataataa gtggatgtac agtcattaca taaccagcca tcataggaga taaatttttt
    23821 aattgttcta aataaaatgg aagtagaaaa gatactgcca tttgaggaaa ataacaagct
    23881 actccaatga tattgcctac agtaaagttt ctatttttaa atagaggtag gtcaataaag
    23941 ctttgttcaa attttacttc tcttaatgcc aacaataaga agattataag tgctgttata
    24001 cctaaataca atcttgtatt acccttctca ttcattgcaa gtaatataag cgtagtgcaa
    24061 gatgcgaaaa gtagaagacc tggaatatca aagctcttgt tttgttcatc caaaacaggt
    24121 ttaggtagaa atttaatgcc aagaaagact gctataatac caataggtat attgataata
    24181 aaaatagaag gccatccaaa atgttctaaa attatacctc caattacagg accactcata
    24241 tttcctatac caactacagc tccagtgatt cctatagctt ttcctttttc atttgctgga
    24301 aatgcattgg aaactatacc aagcccagta gccattaaaa tacttgctcc aactgcctga
    24361 aagattcttg aagaaattaa tgttgataaa ttgtttgaca tactacacag aaaagaacct
    24421 attgtaaata taaaaaatcc taatgtgtat agacgatttg aacctacttt atcgccaagt
    24481 tttccgaaaa ataacatagt agctgttaca acaagcatat aagctgttac aacccactga
    24541 atttgtgaca tgttaatttt aaagtcagca gcaattgtag gatttgcgat atttagtata
    24601 cttccatcaa gagtagacat aaatacacca atacagatga taatcaatac tatccatttt
    24661 tgtttcatga taacactcca atcttaatag attttataag tacgatataa ttgtaaaata
    24721 aacaacaaaa aaagcacctg tatattaggt accttgaatg tgtctaattt catgaataaa
    24781 tacggtagtt gaaggatttg tatagattac tcatgtaaaa ttacataaag gtactcatat
    24841 actattttct tctaccatcc agattttact gtcggctttg gaatatgacc aaattatgcc
    24901 ataaggctcg cgggctttta ccgccgatag ggaatttcac ccgtttatga agaaaaacag
    24961 atgtattttg taatattatt ttaacataaa attataatgg aaacaattgg atttaagtaa
    25021 tatccagctg tattacaata caaatatttc aatatggaca cttaaattgt atttatagat
    25081 agaataagga acagtatgag aatctataat aattagataa aattaacctg ttattagtat
    25141 tagatggtaa gttttaaatt tatcaaaatc agaaagctgt aataaaattc ttataatatg
    25201 aatatcttaa ttaagagata ttgtaaatta tgaggaggcg ctaatatgga aggaaaggta
    25261 aagaaggcat tcccgggtgg aaatacatca caaggctcat attctttttt tgactatata
    25321 attcctgaaa atgtaaaaag agttttttgt ttaaagggtg gacctggagt aggaaaatcc
    25381 tcacttatga aaaaaattta taaagagttt ttgcatagag gatatgatat tgatttatat
    25441 cattgtcctt ctgacccaag ttcgttggat ggtttagtta taaagaaatt aggcgtggta
    25501 cttatggatg caactgcccc acatactatg gacccaaagc tacctggagc tttggatgag
    25561 ataattaatt taggtgatta ctggaataca gaagaattag aaaagaataa acaagaaata
    25621 tcagtttatg ataaagatat tagtaattca tttaaaagag catttaagtt tttaaaatca
    25681 gcagaaccaa tctttagaga catagaagaa aaatattctg attgtatgga ttatggaaaa
    25741 gtaaatttgg tcacagaaga atttataaaa aggttatttg atggagttaa aagcacagga
    25801 aatctaaaaa gagaaaagca tttatttggt tcagctataa ctcctgtagg gtgtttagat
    25861 tatacagaaa acattttaaa agatgtaaag aaagtctatt atttagatgg agaaatagga
    25921 actgggaaaa ctacattatt aaacaaagta tataagaaag ctattgaaaa aggtttaaat
    25981 gtagaagttt atcattatcc acttattcca gagaaaattg aaactgtact attaactgat
    26041 ttagatatag gcataactat aagtacgact tttaaagaag aagatacaat tgatttaaat
    26101 cagtttttaa atagagaaaa actacttaaa tatgaagaag atattaaatt tgatgaaaaa
    26161 gttttagatg atttaatttg ttgggcagta ctaaatctta agagagccaa gtctaaacat
    26221 gatattatag aaagttacta tagtcctaat atgagatttg atgaagtagg gaaattaaga
    26281 gataagttaa ttaaaagaat tttaaaatat gaggaaaact tataaacgca tatgtttaaa
    26341 attaacgtta gtaataatat aatttttagt aaattggtgt agctttgagg ctatgccatt
    26401 ttactttttt tgctaaaaaa tgatattaat aatctccaaa gccattattt gtttttcata
    26461 attattattt cctttcctaa tttatgtttt tttataatat cacaacaatg tttattgaaa
    26521 gtttatataa ttttaagaac atctaaaaat gcatacttga tattggtaaa ttagtgatag
    26581 gagttcaatg tatgatatta tatactaaag atgaatttta atatataact ttgatataat
    26641 aagcatatgt aaatgtttaa tatttaatta aagaaaatta tcatataaaa ataaatttgt
    26701 aaggagcaat acagtatgaa aaaacaatat gtgtttataa tactagcaag tactatttta
    26761 ggtatacttg ttggaaccat atttagagat tatagcaatg aagaaacagt ttatacaagc
    26821 aaagacaagt caataagaaa agaaataaaa atgacaagaa aatctataaa agaattaacc
    26881 aaagagaaag acaagttaga taaagaaatt aattcaatga aacaaaatca aaaaaattct
    26941 gaagaaatat cagaaataga aacacttaga gatagtctat catattcaga tgtaaagggt
    27001 agtgggataa tattaagtat agatgctata gatgaaaaaa cagggaatat agctaatagt
    27061 atagactata ataagattct tgtaaaagtt ataaatgaat taaaagtaaa cggaggaaaa
    27121 tacatagaaa taaatggtca aagaattaat caatattcag aagttgtact tgcaggaagc
    27181 catataaata taaatggagt tcctgtagca caaccatatg aaataaagtg cattggagat
    27241 aaggagaaac tatctaatta tgtagataaa ggtagtaaat atttagatag catacaaacc
    27301 aattatccaa tgaaaataag cataaaaact gaagaaaata tctctatgaa aaaaatgaat
    27361 atacctaata aattaaagta tataaagggt gattaagatt atattagagg aaagcagttt
    27421 gccaatgtta ataccagaaa aacttgcaaa aaaatataat gtatttccag ttgagtttca
    27481 agatgataat ttagttgtag aaatagaaga tgaggatatt tatgctctac aggacttgag
    27541 attagctaca ggtaaagaaa ttatattgaa gaaggagaaa aaagaactta taagtgaaaa
    27601 gattgaaaaa tattataata gctttgaact tgatgaggat tatgcaaaaa aattatttga
    27661 aaatctttta gaaaaagcta taaaagaaaa cgcaagtgat atacatatag aaccatttaa
    27721 agaacattta atagtgagaa tgagggtaga tggagaacta aaggaagtat caaagttttt
    27781 agttgatgtc tatccatcat tgtctacggt tataaaatta aaggcatcaa tggatataac
    27841 agaaaaaagg cttcctcagg atggtagggt ggatataaag ttaggtggaa atttaattga
    27901 cattagagtt tcatcaatac caactgtcta tggtgaaaaa atagttttga ggatacttaa
    27961 tagagatact tttttaaaag acaaattaga acttggtttt tcagaggaag ctataaaatc
    28021 tataaatcgt ataataaata aaagagcagg aatattatta gtgacaggtc ctacaggtag
    28081 tggaaagact acaacagtat attccttatt aaatgattta aaaggaataa ataaaaatat
    28141 tatgactata gaaaatcctg tagagtataa gatggatggc ataagtcaga ttcaagtaaa
    28201 ttcaaaagta gggttgagct ttgatgtagg tcttagagcg atattaagac aagacccaga
    28261 tataataatg gtcggagaaa taagagatgc agaaacagct aagatagcag taagagcagc
    28321 tataacaggt catttagtta taagtacttt acatactaat gatgcagtat cttccatagc
    28381 tagattacta gaaatgcaaa taccaccata tcttttaaat gcctctctta taggtgttat
    28441 atctcaaaag ttggttagaa aagtctgcaa tcattgtagc catgagatta tgataaaaga
    28501 taattttagt ggagatgtaa atactaaggt agctgtaggg tgtgaaaaat gtaatgataa
    28561 aggatacttt ggaagaacag caatttatga aatcttagaa attaatgatg atataaagac
    28621 atgtataaga aatatggagg attctagtac tattaaagat gtagcacaaa ataatggaat
    28681 gataacattt gaagatagtt gtaaaagact aataaatgaa aaaataacga cattagaaga
    28741 gtgtttagta gtaaatgaaa tgatttaact gaattgtata gttaaacttc gtttggtcag
    28801 ttgtctttat ttaatctatt ttaattaaga tgtttgagta taaattatat ttaagtgaaa
    28861 taaaattaaa tgcattaaat tagaaaatat taagtttatt taattgtaat taatataatt
    28921 tggaaggtat tatagtacat gagattatct aaaaatatat tttataattt agaaaagttt
    28981 tatttagtat taaatagaat cagtgataaa gaaattagag taatttgcaa agagataggc
    29041 atattgctgg aatctggatg tgaaattaca aaaatatttg aaatcattga gagtcagtca
    29101 agtaaaaaag taaagaactt gcttagtata gtatcaaatc atattcaaaa aggtaactca
    29161 atagcagaat catttcaaat aacaggtata ttttctaaat tctttataag tatgataaaa
    29221 gcaggagaaa ctagtggaaa cttagatata ataatgagtg atttatctaa ttattatgat
    29281 aaagaataca aattaaaaat gaaaataata actatttcaa tatatccaat aatacttata
    29341 atattatcta tcttatcaat gttgtttata ttcgtatttg taatacctaa ttttcaggtg
    29401 gtttttacca acaatggaat tgaaccacct ttaataacta gagttttaat gggaatatct
    29461 acagtggtta caaataactt agcttatata atatttagtt ttatattgtt ttcaatagga
    29521 agcatttatt tctttataac aaatgataat ataaaaaaat tgattaattc gttaaaattc
    29581 aaaatacctt ttataaaaaa aattaatcag ttagtagcta caacaagatt ttcaagggca
    29641 ttgtccgttt taattagcag tggaatacaa attgtggaag ctatagatat gtcagctagt
    29701 gttatagaca acgaatttat ttatgaaagg ttaaagattt ctaataatta tataaaaaaa
    29761 ggaaatagca taggtaaatc tttgaattta gcaaatattt ttccaaaatt atttatttca
    29821 atggcaaata caggagaaga aagtgggaaa ctagataaaa gtctaaatgt aataaataaa
    29881 ttttatgaaa atgaattaaa taacaaaata gagcaaataa tgaaatttat agaacctatg
    29941 ctaattgtat ttatgggatt aattattggt acttttattt tagcaatggt tgttcctatg
    30001 tttgatgcaa ttactaattt ttagagagaa aaataaattt tattagatga aacttttgaa
    30061 aaggagagaa tatttaattg ataaatttga taaataaaaa acgaaaagga tttacacttg
    30121 ttgaaatgat tgtagtagta actattttag gcgttatatc tagtatagca ttagttaagt
    30181 atagtaaggt tcaagaaagt gccaaattaa atgcagacta tacgaatgct gctaatatag
    30241 taaccgcagc tagcatggca attaatgatg atgaaaagac aatagactct ctaagtgtag
    30301 agacattgaa ggaaaaggga tacctaaata ctgttccagt tcctcagagt acatcaggta
    30361 aattcgaact tgtcataaat gatagcggaa cagatataag cgtaaatata aattctaagc
    30421 aattttatcc aaaataatat tttatattga ttgctgtaag ttttaaaatt atttttattt
    30481 aagaaggaga tggctataat taaaaaaatt ctaaacttag aaataaatga tgattatata
    30541 aggcttgtat ttttaagaaa aagtcataaa aaagttttta tagataaatc aattataaaa
    30601 gagattaact ttgatataaa aaatgatagt aattcgattg agaagattaa atatatagaa
    30661 gatgtagtta gatttgtaaa agaggagatt ggtaaatcaa aaatattata taaaaattta
    30721 tactttaaca ttcaaattca ggatatagtc attagaaatg ttgaagttct aaatactaag
    30781 aaaaaaagag acatattaag catgattgaa tttgaaataa cacaatacat tccaataaac
    30841 ataaataact attcaataaa atataaagtt ataaattcta aaaatgataa attgaatata
    30901 caagtaattc ttttcccaaa gaatattatt caattaatta agaacatttc agaaaatcta
    30961 aatatgaatc caaagactat aaatataaat tttgatatat tacaaaagct tataaactta
    31021 aatttaatag agaatttttc agaagaaggg gtatttatag aatgtaaaaa aagtcagtta
    31081 attataaata taacaaaaga taaaaaaata agagaaacat acttgctatc aaaagaggga
    31141 aaatcatatg agttcataaa aagattatta aggggtttta agtatgttta ttactatggt
    31201 atagaaaatt gttttattga agaatacttt aaatatacag ataaatctat tgaagtaaat
    31261 ccattaagat taaaagataa agtaaaagta gaaaataatt ttgatgcaga tttagaagac
    31321 aataggataa attatataaa tagtatagga atgattattt aagtgaaagt ggataaaaca
    31381 ataaattttc ttaatggatt taaaaaaact aaaccatgta ataaaatcta taaaataata
    31441 atagtcttaa ttttaacgtg tattttattt aaatcattta tgtatgtgaa atctataaat
    31501 gaacttgaag attatataag tcaaaataag ctagtggata aatcaaagaa cactttacca
    31561 acaacagata aaactagaga gaaattttct aaaataaaac ttaatgaaat aaaaaaaata
    31621 tatgaaacaa ttggaataaa tagtattact aaaatatcaa tagataaaaa taagctagag
    31681 atggaaggtg tgtgtagtga tttagatgtt ttaaacaatt taaggaaaat aaagtacata
    31741 aaatattatt acatagacag tattgtaaga gatggagaat attatagatt tagaataaaa
    31801 agttagatgg gtgattattt ttgagattaa ataatagaga aaaaaatcta cttaaaatat
    31861 tgttaatatt agttattata ttggcaagtg atatagcagt aagttatttg gaagaaaacc
    31921 taactagttt gaagaatgaa aagcaaaata taataaataa aataaataat caagataatt
    31981 atcaaataaa ggataataag catatagatg ataaatatat agaaagtgtt gaattaaata
    32041 aaaatgaaga attgaaaaat aattttaata actcattttt agataaaaaa acaaataaag
    32101 atatagtgtt attagtagaa aatgaactga gagatattgt gaatgtagaa tatattaatc
    32161 aagaaagtag tttagataat cacaataaaa aacaagttaa tttagaaata aaagttaatg
    32221 gagaacttgc aaatgtgttg aaacttgaag gtgtaatgaa gaagttaaga ctaaataata
    32281 aacttgaaaa gatagaaatt aacaaattaa aagtcaataa ttttgatgtt caaaatattc
    32341 caaatgaata taaacaagaa aaagacaata ttgttgaatg taaaatgaca gttagagtca
    32401 tataacaaaa aagaatagat aaagtaaaaa actattatca attaataaaa ttttatagag
    32461 gaattagttt tacgataatt catttctaca agagggattg ttgatgattt tatcgaaatc
    32521 ctaattaaag ttaatatatt gtaccaaaat ataagtgaat ttaatagaat atttacttat
    32581 actatagatt gattaataaa aaaatggtat aataacaaat tgtactttaa tttaataata
    32641 agggagtgga agtcatattg ttagggagaa atgagctatg cccttgtgga agtggcaaaa
    32701 aatacaagaa atgttgttta aataaagata ttgtttcaga aagagcatgc agaaaaactg
    32761 aactttcaca aaaacaatat acagatttat attcaaagtt atatgattat tcaagacagg
    32821 ataagtttaa agaagaatgt gaaaaagcta aagaaatgtt ttatataatg caaaatgata
    32881 gtgtaaatga aaaatttgaa agatttttta atacttattt aatacaagac cacataatgg
    32941 aaaataaaaa ggttatgaca gttggttttc ttgaagaaaa tggtgcaaac ctaagtcaat
    33001 cagaagtatc aatattaaaa agtatgtttg aatcatacgt aagtatttat gaggtaaaag
    33061 aagtttctgc tggaaaaata gtattaaagg actgtttaaa tggaaatgaa ctttgtacag
    33121 aagatgtaaa acttctgaga agttttaaaa tcggaagttg tatgatagct agaatagttg
    33181 atattgaagg aactaacata ttgatagata taactataag tatatcaaat gaggttaaag
    33241 atataatttt aaaggatatt atgaatctat tcaatcaata tcaagatgta tacaaagata
    33301 tgaagtcgtt cttaatttat catacacata tactatacaa gtatatacaa cagcttttag
    33361 acccaagtat agctgaatat ttaaagagag aaagagataa aaacgataca aaagttaatt
    33421 cagaaaatga attagctgaa gaagaatgta aagttttaga tactcttaaa gaaaatgtag
    33481 aaaaagaaaa tatagatgca tgtatagagt tttggaatga atataagaaa aatcatgaaa
    33541 atataaaagg agctgaaaat ggttgggcag ctgctgttga atactatgtt aaaaaagaag
    33601 caggacaacc tataactcaa gttcaaatat caaagaaata cgatataagc tctagtacac
    33661 taggaaagag atacaaggat ttaaaaatat cttaattaat tataaaaagc tttaagtcat
    33721 agataaagaa tctatagtat aatatatgta taatgtttag aaagaggtat taataatgag
    33781 tcttagttta ggaaatgcaa ttgctgtatc aacgaacttt ataaaatcta acccagctgt
    33841 acctgtgtac aacggagaag aaatatatgc agttatttat catatagaaa taggatataa
    33901 taatggtgta tcacctgata ccaaacaata tataataata gactataaag agtttgattt
    33961 atctatggat aaaaaaaatg aactaataaa tattgctaaa gagtactgta aatctaaagg
    34021 tattgtagaa aacgaaaacc ttgaaataaa ccttttagac aatgaagaag cagagaactt
    34081 cttagaaaag gtgtttaata atatgaaggt tataagaggt cttataacta aaaaaggata
    34141 aataaatgga ctatatagga atagaaaata taacacctta tgaaaataca tatgaattta
    34201 gtgtatatga atatgatgat gaaatcacct taggtagtga aaagttatat gtatgtgaat
    34261 taagggttgt attgattaaa gttaattctc tgtatgttga aagattgcat aaatcagttg
    34321 aagcaatggt cttagtaaaa aacttgaaaa aagatttaga taaaacactt gttgtaaaca
    34381 aaataaagaa ttttgtgcta gatgagattt gggtagaaaa tctagtaaaa gagaatatag
    34441 aagttatatt tgtagaaagc taggaataac cctagctttt ttatattaca atctaagaga
    34501 ggaaaacaat atgttaagag tatctaatgt aaaacttgga attgataaag atatatcttt
    34561 attaaaagat gttatactta agaagctaaa aataaaagaa aaaaacttag tcaaatatag
    34621 tatatataaa gaatctatag atgctagaaa aaaaggaaaa atggaatttg tttattctgt
    34681 agatgtagaa gttaaagatg aaaataaaat acttaactca aaaataaaag atgtaacaaa
    34741 aataaaggaa atcagatacg taaatgtacc tgtgggtgat aaaaaattaa agaataatcc
    34801 tttggtaatt ggtagtggac cagctggctt gtttgcaggt ctgcttttag ctcaaatggg
    34861 ttatgaacca atcatacttg aaagaggtct tgatgtagac agtagaacca aagatataag
    34921 tgatttttgg acaactagaa aattaaaaaa taattcaaac gttcaatttg gagaaggtgg
    34981 agcaggaact ttttcggatg gtaaacttac aactagaata aaagatatac gatgtagaaa
    35041 agtattagaa gagttagttc agtttggagc accagatgaa atattatact cacataagcc
    35101 acatgttgga acagatatat taaaaaatgt agttaaaaat ataagaaatg aaataataag
    35161 tttaggtggt caagttagat ttaattcaaa agtaacagat atagttgtat caaatgattc
    35221 tatacaatct gtaataataa atgatactga aaaaatagat acagatacaa ttatattagc
    35281 tgttggacat agtgcaagag atacttatga aatgttgtat tcaagaggta taaaaatagt
    35341 tcaaaaacct tttgcaatag gagctagagt tgaacatcca caagatttaa taaataaagc
    35401 acagtacaag gatttttata atcatcctag gttaggagct gcagattata gacttataga
    35461 gcatgtttca aatggaagaa cagcatatac tttttgtatg tgtccaggag gtagtgtcat
    35521 agcatctgca tcaaatgatt ttgaaatagt tacaaatggt atgagtgaac atgcaagaga
    35581 taaaataaat gcaaatagtg catttttagt aaatgtaacg ccagaagatt ttaagagtga
    35641 taatccacta gcaggtgttc atttccagca gaagtatgaa aaattagctt ttgaattagg
    35701 tggaaaaaat tataatgcac cagttcagct tgttggagat tttataaaca ataagacttc
    35761 tttgaattta ggaagtgttg agccatcata tagaccagga tatacactta caaatttaag
    35821 tgaatgtttg cctagcttcg taacagagac tatgagagaa gcgtttatat ctcttgataa
    35881 aaaattgaat gggtttgcaa tgtatgatgc tgctttgacg ggagtagaaa ctaggtcatc
    35941 atcaccaata agaatagtaa gagatgaaaa tactttagaa tcagtgagta ttaagaatct
    36001 atatccatgt ggtgaaggtg ctggatatgc aggtggtata gttacagcag cagttgatgg
    36061 tataaaatgt gcagaaaaaa taatacaaaa atacaccaat aatacaaaaa tttaacataa
    36121 acttaatgat tttttaatat ttaacatgaa tttaacatta tcactcctat aatttgataa
    36181 aatacaatag gttataaaat ttttagatac agtaatctct ttacaaggag gaaataattt
    36241 gaacattata ataagtctaa tgggtggatt aggtctgttc ttatatggta tgaaccttat
    36301 gggagaaggt cttcaaaaat ctgctgggac aaagctaaaa aagataataa aactacttac
    36361 tagtaaccta tttatgggag ttttggtagg tacaggagtt acagcagtaa tacaaagttc
    36421 aagtgctact actgtaatgg ttgttggttt cgtaaatgca ggaataatga ctttaaaaca
    36481 ggcaatagga gttataatgg gtgctaatat agggactact gttacagccc aattggtatc
    36541 tttcgactta actggtatgg ccccagtagc attaggtgta ggtattatac tttatttatt
    36601 tggaaataag cctagaataa aaaatatagc cgaaatactt ataggttttg gtatattatt
    36661 tacaggtatg gattttatga agatggcagt ggagccacta agagactatc aaggatttac
    36721 tgacttactt gttacatttg gtagatatcc actacttgga ttattattgg gatttgggat
    36781 tacagcaata atacaaagtt caagtgcatc tatgggtatg ttggttgcac tggcagcaga
    36841 aggtctagtg ccattatcag cagcactgcc aattctttat ggccaaaata ttggtacatg
    36901 tgtaacttca ctgttatcaa gtattggtgc taacaaaaat gcacgaagag cagctatgat
    36961 gcatctaatc ttcaatgttt taggaacggt gatattctta atattcttga ataaaccagt
    37021 agtaagcatg gttacaagtt gggacccaag caatgttgca agacaaatag ccaatacaca
    37081 tacattgttt aatataatat ctgtattgat acttttacca tttacaaatc ttattataaa
    37141 actagctata aagcttgttc ctgatagagc aggtgatata gatgaagatg aaactaaaac
    37201 tattaaatat atagatgata gaatgataga aactccttct atagctcttg caaatacgat
    37261 aaaagaagca cttcgtatgg gtgagaaggc gaaggaaagc ctaaatgcat ctatggaagc
    37321 tttagtagaa cattcaactg aaaaaataga taagacatat aggcgagaga gattaatcaa
    37381 tgatttacag aaagctattt taaattattt acttaaactt tcaaaagcac ctttagatga
    37441 tgattcaaga gaagttgtgg atactttgtt taatacagtc aatgatatag aaagaattgg
    37501 tgaccacgct aaaaatatag ctgaattatc gcaagttgca atagattcaa atatttcttt
    37561 ctcagaagaa ggacaatctg aacttgatgt tatgtataat agagttgttt cagcatatac
    37621 ttatgcatta gaatcaatga gaactgataa tgttgattta gcttgtaagg ttataaaaat
    37681 tgaagaacaa gttgatataa tggagaaatc ttgtagagct aatcacatgt atagattgaa
    37741 caataatcta tgtagtatag aaaatggagt tatttattta gacgtaataa gtaatttaga
    37801 gagaatatct gaccatgctg tcaatatagc acaacaagtt atagcaaaga gattaggaaa
    37861 tgattaaaaa taattaattt aaaataatat aaaatttaaa atagaggata aataaaagag
    37921 aaactttaaa tgatacgttt agagtttctt tttttgtaaa tttatttgac taaaattttt
    37981 aaatttatac atagttatag catttagaag aatcgatttc ctgataataa atgatgagat
    38041 atgtatgatt ttgtggcaaa cttttaaaaa aaacacctat attttgtgtt ataatctctt
    38101 taaaacgaat atgctgaaaa gagggattta aatgggaaaa ataaattttg attatagtaa
    38161 agctacagag tttttttgtc aaaacgaaat agatgtcatg caaccatatg tagatgttgc
    38221 gcacgacatg ttacataata aaactggttt aggaaatact tttttaggat ggatagacct
    38281 tcctaaaaat tatgataaag aagagtttga cagaataaaa aaatctgctg ataagataaa
    38341 atcagactca gatgttcttt tggtaatagg tataggaggc tcttatttag gttcgagagc
    38401 tgctatagat atggtaagtc attcttttag gaatggactt aaaaaagagc aaagaaaggc
    38461 tccagaagta tattttgtag ggcataatat aagctcaaca tatattatgg atttattaga
    38521 tataattgaa ggaaaggata tttctgtaaa tgtaatatct aaatcaggaa ctactacaga
    38581 accagctctg gcttttagaa tatttaaaga ttacttagag aaaaagtatg gtaaggaaga
    38641 agcaagaaag agaatttatg caacaacaga tgcaagtaaa ggtgcactta gacagttagc
    38701 tacagaagaa ggatatgaga cttttgtaat accagatgat gtaggtggtc gtttttctgt
    38761 attgacagca gttgggttac ttcctattgc tgctgctgga cttgatattg atgctatgat
    38821 gaaaggcgca aatgatgcta gagaagcatt tcaaaatcct gacttaaaaa gtaatgatag
    38881 ttatagatat gctgttgcta gaacaatttt acatagaaaa ggaaaagatg tagagttatt
    38941 agtgaactat gaacctcaac tacattatgt aagtgagtgg tggaaacagc tttatggaga
    39001 aagtgaagga aaagaaaata aaggtttatt ccctgcttct gtagatttct caacagattt
    39061 acattctatg ggtcaataca tacaagatgg aaaaaggctt ttatttgaga cagtacttaa
    39121 tgttgaaaac tgtaagagaa atataactat aagttcagag gaagtagact tagatggttt
    39181 aaactattta gcaggtaaga ctgtagattt tgtaaatcat aaagcttttg aaggaactct
    39241 attggctcat acagatggaa aagtacctaa tttagtaata aatatacctc aactagatga
    39301 gtataatttt ggatatttag tttatttctt tgagaaggct tgtggaataa gtggatattt
    39361 attgggagtt aatccatttg accaaccagg agtagaagct tacaagaaaa atatgtttgc
    39421 tttactaggc aaaccaggtt atgaaaaaga aaaggaagag ctagaaaaaa gacttaaata
    39481 attttaaata gttaaggttt aattaagatt aaaataatat aatgaaatca tcgaaaagga
    39541 gtttactcaa aatagatgat ttcatttttt ttatgaaaaa ttgggaaaaa tatattttga
    39601 taatttatat tatattaaga tagggagtgg tatgagatgt caagaagaaa gaaagggata
    39661 agtttagtaa tattagttgc tataataagt tcaatactta gtagttttct aacaataatt
    39721 ttagttaagg acaatttagt aagtaaatct acaggaagtt ctacgccgat agttgtaaat
    39781 gatgatggta agagtcaaaa tatttatcaa gcagttgcag aaaaagctac gccatctgtt
    39841 gtaggtatta caacaactag tgttgatact agtaatatgt ttgcaatacc aactgaaaca
    39901 caaggtgtag gaactggaat catagttgat tcaaatggat atatacttac taattctcat
    39961 gttatatcag atggtcaagc tacaagtgta aatgtattat tcaatgatgg ttcaacaact
    40021 tcaggtaaag tagtatggtt tgaccaacaa cttgatttag ctatagttaa ggttgataaa
    40081 actggactaa caccagcaga atttgctgat agtgacaaag taaaagttgg agatatatca
    40141 atagcaatag gtaatcctct tggattagat tttcaaaaaa cagttactca agggataata
    40201 agtggattag atagaactat acaaacagaa aaaactaata tgactggttt attacaaact
    40261 gatgcaagta taaatgcagg aaatagtgga ggaccattgt taaatcaaaa aggtcaagta
    40321 attggtataa acacagcaaa agcttctcaa gcagaaggat taggatttgc aataccaatc
    40381 aatacagcta agagtatagt tgaagaagta atcaaaaatg gaaaatatga aaaagtaact
    40441 ttaggcataa aaggtacaga tgtatcaaat tatgaagcag caacaggtac aaaattaagt
    40501 actgataaag gtgtttatgt tgcagaagta atatctggtt catcagcaga aaaagctgga
    40561 gtaaaggtag gggatattat aactaaagta ggagatacag atataacagg tatgaatgat
    40621 ttaaataaaa agttgtatac tttctcaaaa ggtgctagta caaaaataac agtaaataga
    40681 ggcggaaaag cagtaacaat aaatgtgaat ttctaaatat gaccagaaag atagagacta
    40741 catggtaaag tgtagtctct atttttattt aatttttata gtatatacat ctaaattaat
    40801 taaaatgatg tattgtttat agtttattgc tatattttct tataaaaata aatttaataa
    40861 atattt
//
LOCUS       DAYAPA010000023        40122 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.023, whole genome shotgun sequence.
ACCESSION   DAYAPA010000023 DAYAPA010000000
VERSION     DAYAPA010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 40122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40122
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.023"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(<1..557)
                     /locus_tag="ACGI59_001084"
     CDS             complement(<1..557)
                     /locus_tag="ACGI59_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFG9738888.1"
                     /translation="MFKKIVAIASSIIVSTICLTGCDFNSNDKKGESSSSKNLDKVTI
                     AEVTHSVFYAPQYAAITKGFFEDEGIKIDLINTQGADKTMAALISGEANVGLMGPEAS
                     IYVYNQNKNDYAVNFAQLTKRDGSFLVARKKMPNFSYNDLKGTEILGGRKGGVPLMTF
                     EYVLKEKGLTIGENLKAGNVNVRTDV"
     gene            943..1677
                     /locus_tag="ACGI59_001085"
     CDS             943..1677
                     /locus_tag="ACGI59_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFG9738889.1"
                     /translation="MKQNKYDDDRFFNKYSKMERSTNGLAGAGEWHVLKKMLPDFKGK
                     RVLDLGCGFGWHCQYAVENGAISAVGVDISEKMLKEARNKTQLDNINYICMPIEDINF
                     PKDSFDVVISSLAFHYIQSFEDICKNISNCLSNKGNFVFSVEHPIFTAYGSQEWYCDK
                     DGTNLHWPVDGYFEEGVRKSNFLGEEVIKYHKTLTTYLNTLMKTGFEILEIIEPQPEE
                     KLLDTIPDMKDELRRPMMLLVSAKKR"
     gene            complement(1820..2311)
                     /locus_tag="ACGI59_001086"
     CDS             complement(1820..2311)
                     /locus_tag="ACGI59_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HFG9738890.1"
                     /translation="MNNQQQEVYKKLDELSIPYEAVNHPPVYTIEEMEELKTLHMEFV
                     VKNLFLRDAKGKEHFLVVLGKDKKADLKDIRQQIDSKPLSFASEERLQKYLKLTKGAV
                     TPFGILNDKEAVVKVVFDKDLLKMDKIGIHPNDNTATVFLKFEDMKKLIEENGNEIYY
                     VEV"
     gene            2660..3043
                     /locus_tag="ACGI59_001087"
     CDS             2660..3043
                     /locus_tag="ACGI59_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBECR2 nuclease fold domain-containing protein"
                     /protein_id="HFG9738891.1"
                     /translation="MSTDKVVGIIDEETAELAGIEYTGKIYASSGVIKHIKKKHRCQL
                     SKDIFNDIIDTIKMVLKSPEYIGSHPKKPGKSVEFIKKIDDYILVATELDTKKGYLYV
                     SSMYAIKEAKLESRVNSGRVKQYNG"
     gene            3551..3934
                     /locus_tag="ACGI59_001088"
     CDS             3551..3934
                     /locus_tag="ACGI59_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFG9738892.1"
                     /translation="MNLSKKIEDKRKAKQRAEKVKKAKIATAGVVLGAVTGAVSGVLL
                     APKSGKETREDIKDASQQIAEKINMKTVDVKGKVSEKLEDKKGNFIESKKKIKKYIDN
                     KKSVENSSEEDKIIDIVEPVSVTEE"
     gene            3936..4286
                     /locus_tag="ACGI59_001089"
     CDS             3936..4286
                     /locus_tag="ACGI59_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9738893.1"
                     /translation="MDNTITINISIIYVLLAILACIALVYLIIVLVKLHKILNNVGTM
                     LNENTQNINTTLNNLPNVVETFGEVGENMKDVTEVVTEVTADFLVTKDSVKSNMDVIV
                     DILTIIKNIFLDKK"
     gene            complement(4387..4845)
                     /locus_tag="ACGI59_001090"
     CDS             complement(4387..4845)
                     /locus_tag="ACGI59_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9738894.1"
                     /translation="MIRTKVVELIATVCRENKPHKWVDENYTPYDKSGKVELMSIEDL
                     NELISSSGRADFLYSSRLQKLLEEVYINQSRASYISGCGLFWSSYWDILEEKFEEWLY
                     NSYIFFDEDDEYLEGMEDFELECKDVLMDVIETTSIDIYVQMIKRNITNY"
     gene            complement(4890..5678)
                     /locus_tag="ACGI59_001091"
     CDS             complement(4890..5678)
                     /locus_tag="ACGI59_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFG9738895.1"
                     /translation="MKVVIIPGHTLIGKGTGAVGYINESKETRILNDLIVKWLKIGGA
                     TVYTGRVDESSNHLADQCAIANKQETDLAVQIHFNSNATTSTPVGTETIYKTNNGKTY
                     AERVNTRLATVFKDRGAKSDVRGLYWLNHTIAPAILIEVCFVDSKADTDYYVNNKDKV
                     AKLIAEGILNKSISNSQEGGENKVYENVIVYTGDADKVAAQILHWQLKDSLIIEASSY
                     KQGLGKKVYVVGGEANKLVKGDVVINGADRYETVKLALQEIDKL"
     gene            complement(5817..6254)
                     /locus_tag="ACGI59_001092"
     CDS             complement(5817..6254)
                     /locus_tag="ACGI59_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HFG9738896.1"
                     /translation="MNKKLENRCSWQEYFMRLCETVAERGTCDRAYVGAIIVNSENRI
                     VSTGYNGSISGDKHCSEVGHEMRDGHCIRTIHAEQNALYYCAKEGISVKNCSIYVTHF
                     PCLNCTKAIIQAGIKHIYYRTGYRIDEYAIKLLQSSNVLYTKL"
     gene            complement(6488..6622)
                     /locus_tag="ACGI59_001093"
     CDS             complement(6488..6622)
                     /locus_tag="ACGI59_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9738897.1"
                     /translation="MGILEGAAMIREIAIKIAKEKGITEQKAWPEAVKEFKEKYELVL
                     "
     gene            7076..7585
                     /locus_tag="ACGI59_001094"
     CDS             7076..7585
                     /locus_tag="ACGI59_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFG9738898.1"
                     /translation="MLSENIKTIRKSKGLSQQELAIKLNVVRQTISKWEQGLSVPDSD
                     MLISISEVLETPVSTLLGETIVESEVDNLKVISEKLEIINLQLAQRKNMRRKMFHWLF
                     ILLCAVILIVFVVLGGLNSPYLDWNYTDPETAVFGVAFHAFEWLFVRLVPIVLIGAII
                     GIFLTRKKI"
     gene            7612..7758
                     /locus_tag="ACGI59_001095"
                     /pseudo
     CDS             7612..7758
                     /locus_tag="ACGI59_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            7991..8527
                     /locus_tag="ACGI59_001096"
     CDS             7991..8527
                     /locus_tag="ACGI59_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFG9738899.1"
                     /translation="MQGGVNIRQVKSFVFPVISLIFLDQISKVLIGLFLMDFEIDIIG
                     KFLRFNPVQNTNLSYGGNFIGILSNLWVLVLFNILVILVIISGYAFYKSKNEQTSYSV
                     KVIMSCGLAGTICSLIDKLFWGGSLDFLQIPSFFIFDLKDCYLTVAEIIFVVIGILHN
                     REISMKEYIYFCYRQFKK"
     gene            complement(8954..9754)
                     /locus_tag="ACGI59_001097"
     CDS             complement(8954..9754)
                     /locus_tag="ACGI59_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9738900.1"
                     /translation="MRLNKRSEGYINYVKGVKREKRNVLFYQLLILIGFIVIWELLAD
                     LNVINTFLFSKPSDIYNLFIQYAFSGQLFKHIGISVYETVLGLVIGTVLGILVAIALW
                     WSEKLSKILDPFLVVLNALPKTALAPIIIVWVGAGIEGIVVTAVTISVVVTILSAYNY
                     FMNIDEEKIKMLKSFGATKSQILFKLILPANTGNLINLTKINIGMAWVGVIVGEFLVS
                     RYGIGYLIVYGSQVFKLDLVMMGVFVLAICAWAMYAVVNIIEKIYNSR"
     gene            complement(9949..11547)
                     /locus_tag="ACGI59_001098"
     CDS             complement(9949..11547)
                     /locus_tag="ACGI59_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFG9738901.1"
                     /translation="MKAAIYSRKSRFTGKGDSIENQIQLCIDYAKSIGVKDFLIYEDE
                     GFSGSNIDRPQFKKMMQDAKDKKFSYLVCYRLDRISRNVSDFSTLIEKLNELSISFIS
                     IKEQFDTSTPMGRAMMFISSVFAQLERETIAERIKDNMYELARSGRWLGGNTPHGFNS
                     EKITFLDENLKERSMYKLQVNDEQMEIVKLIYNKYLELRSLSKLYKYVYNNGIRGPRG
                     GKFDPSSLSRILRNPAYVKANDEILDYLKNSGMDVVGTPDRKCGILTYAKNTSNSIAA
                     IAKHHGVIEPEIWLEVQNQLDKNRDKTPRISTGKTALLSGLIKCGKCGSNMRITYKGK
                     KTDKDLKYYYICGTKKTLGSDSCNCKNLNGPLVESLVIDKIKNCKEESIISAFNSNNI
                     KTDNVFNIFKSETNLLKNQILDKERLIKNLVIELAKNTGSTASDYIVSQIESLNNEIS
                     ILKDKINTLESSNINISSDSINIDIIVSNLKRFNDEVDNASLDDKRLLLSTIINSIVW
                     DSSLGNLNIVYMGAEVDNSVSLSN"
     gene            11875..12051
                     /locus_tag="ACGI59_001099"
     CDS             11875..12051
                     /locus_tag="ACGI59_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9738902.1"
                     /translation="MEINVIEIFPKDKAKLNKIEMDKASWFVNIISKKYPKEALNEAF
                     STLEKELNISKANT"
     gene            12225..13355
                     /locus_tag="ACGI59_001100"
     CDS             12225..13355
                     /locus_tag="ACGI59_001100"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0051144 - propanediol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-propanol dehydrogenase PduQ"
                     /protein_id="HFG9738903.1"
                     /translation="MNNFEIKTRIKFGEGSLKALQELKNKNVLIITDPFMVKSKTIDK
                     ITSNLINSTYQVFEEIVPDPPIEVVVAGIEAFKSINPDVIIALGGGSAIDAAKSIMDF
                     SKKILKINEVEFIAIPTTSGTGSEVTSFAVITDSQKKCKYPLVSDDLLPDMAILDPEL
                     VKTVPNFITADTGMDVLTHAIEAYVSTNSNDFSDALAEKAIKLVFKYLIKAYKDGNDI
                     EAREKMHNASCLAGMAFNQASLGINHSIAHVLGGKFHIPHGRTNSILLPHVIEYNSSI
                     VGYTNTTYSATAKKYAQIAELVGLGSANIRISVRNLVNEIKKLQKEMNMPTKLTQCKL
                     SLEDITNEEREIAKLAIKDTCTLTNPKVPTEDDIINILKNIK"
     gene            13596..13946
                     /gene="eutS"
                     /locus_tag="ACGI59_001101"
     CDS             13596..13946
                     /gene="eutS"
                     /locus_tag="ACGI59_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HFG9738904.1"
                     /translation="MTEESKQRVIQEYVPGKQVTLAHIIANPNEDIYKKLGLVLDKKD
                     AIGILTITPSEASIIAADVATKASNVSLGFIDRFSGSVVISGDVSSVESALNDVLEVL
                     GNMLNFSSTKITRT"
     gene            13954..14403
                     /locus_tag="ACGI59_001102"
     CDS             13954..14403
                     /locus_tag="ACGI59_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HFG9738905.1"
                     /translation="MKNIIFMGKTGSGKTTLCQKLDQLELKYKKTQSVELYNNAIDTP
                     GEYMENRNLYNALITTSADAKIIAIVYDATQEENYIAPGFASMFCKDVIGIITKINKA
                     TENEVNIAYERLSLAGASQIFKIDTVDNIGLEKLFKYLEKYKNIDEV"
     gene            14505..15080
                     /locus_tag="ACGI59_001103"
     CDS             14505..15080
                     /locus_tag="ACGI59_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFG9738906.1"
                     /translation="MGKKILLVDDEPLLRMDTKDILESHGYEIIGEASDGFEAVEMCK
                     KHKPDLVIMDIEMPILDGIKAGKIICKENIAGGVLLLTSFEDKEYVEKAKSIGAYGYL
                     VKTASEKSLIPTIEMCLNKVNEFEKMKKDLEKVNSKLNERKLVERAKGILIREFKISE
                     DEAYTRIRKLSMDKRTTMMEIAKMIIIGYDD"
     gene            15073..16482
                     /locus_tag="ACGI59_001104"
     CDS             15073..16482
                     /locus_tag="ACGI59_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal domain-containing
                     protein"
                     /protein_id="HFG9738907.1"
                     /translation="MTNTIKKISTAQTYLNNNEIEKIIEVSKSLDLMASFYEADVFID
                     VLSKFDDEAIVVAHGRPKEKSMYCENVIGKKALIENEPGVIKCLKTGERVRDIKALTQ
                     EYKLVKQNIQPITLDDKVLAVLIVEKDISSEVKNEFNASEDEENKNLHQFIKLIGNND
                     FLVDNLNSAILIFDKNGKLKIKNRIATEIYKNLGFGYDIQDMHYNDLDIETNTFENIL
                     MDENYDETNEVSIGNMFFKIKTIYIKDDEFKVCKIVQDISDLKRKEEELVLKTVAIRE
                     AHHRVKNNLHTVISIIKKQSRLSQNDEVKQCLDNIINRVFAILSTHYLLSKEVDNNIS
                     IREGINLLVSNIQGGYLFSKDIDIRITGEDFKISGEKATAILLVINEILQNCYDHAFS
                     DREHGTIQILINKDNDYRNIAIIDDGIGFDSNNINKNSLGTYIIDSYITQMLKGNIER
                     KSSENGTEVLITIPTQINI"
     gene            16666..18099
                     /gene="eutA"
                     /locus_tag="ACGI59_001105"
     CDS             16666..18099
                     /gene="eutA"
                     /locus_tag="ACGI59_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HFG9738908.1"
                     /translation="MREELLSVGIDIGTSTTQLIFSKLIVENTESNFSVPRISIVDKE
                     IIYKSKVYFTPLISNNEINGQQIKDIVENEYKKANISKEDIHTGAVIITGETARKENA
                     NDVLHTLSGFAGDFVVATAGPDLESIISGKGAGAHIYSKEHSTTIVNIDIGGGTSNIA
                     SFKRGEVTDTGCLDIGGRLVKIDKISKKITYISPKIDKIIKDNNLNLQVGTMATVENT
                     KPIVDIMVDLLMESIGLKKQTPLFDFIITNKSISIDENIKNVSFSGGVADYIYYDGEI
                     KDYFEYGDLGILLGQAIKNCEYLKKVNVVKSMETIRATVVGAGSHTTEVSGSTITYTS
                     EKFPLKNLPVLKLSIDEETGTSKQISEAIKNKLNWFKLENDLQKVAIAMIGKKNPTFL
                     DIQEYAKGFVDGMQQIIENDLPFIVIVENDMAKVLGQAIYALLNYEKEIVCIDSVKVE
                     NGDYIDIGKPIVEGKVLPVVIKTLVFN"
     gene            18117..19481
                     /locus_tag="ACGI59_001106"
     CDS             18117..19481
                     /locus_tag="ACGI59_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HFG9738909.1"
                     /translation="MILNTKLFGRTYQFKGLYDVMAKANEEKSGDKLAGLAAESAEER
                     IAAKVVLSHIKLEDIRNNPAVAYEEDEVTRIIQDAVNEKVYEEIKNWTVSELREWLLD
                     SNTTSLDIRRISRGLTSEMIAAVAKLMSNMDLVYAAKKIKVTAHCNTTIGEQGTLSAR
                     LQPNHPTDDPDGILASLLEGLTFGIGDAVLGLNPVDDSVESVTKVLKRFEEVKQKYQI
                     PTQTCVLAHVTTQMEAIRNGAPADLIFQSIAGSEKGNEAFGFNGDTIEEARQLALKHG
                     TATGPNVMYFETGQGSELSSEAHHGIDQVTMEARCYGFAKKYEPFLVNTVVGFIGPEY
                     LYDAREVVRAGLEDHFMGKLHGLPMGVDVCYTNHMKADQNDMENLAILLTTAGCTYFM
                     GIPHGDDVMLNYQTTGYHETASLREMFGLTAIKEFDAWMEKMGFSKDGKLTELAGDAS
                     VLLG"
     gene            19494..20375
                     /gene="eutC"
                     /locus_tag="ACGI59_001107"
     CDS             19494..20375
                     /gene="eutC"
                     /locus_tag="ACGI59_001107"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008851 - ethanolamine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutC"
                     /protein_id="HFG9738910.1"
                     /translation="MNEKDLKALVEQLVGQMVGELDTNVVSETVKKATEVVVDNNACI
                     DDITEVDIRKQLLVKNPKDAEAYLDMKAKTPARLGIGRAGTRYKTETVLRFRADHAAA
                     QDAVFSYVDEEFIKENNMFAVETLCKDKDEYLTRPDLGRKFSPETINNIKSKFGTNQK
                     VLILVGDGLSSAAIEANLKDCVPAIKQGLKMYGIDSSEILFVKHCRVGAMDHLGEELG
                     CEVICMLVGERPGLVTAESMSAYIAYKPYIGMAEAKRTVISNIHKGGTTAVEAGAHIA
                     ELIKTMLDKKASGIDLK"
     gene            20395..21048
                     /gene="eutL"
                     /locus_tag="ACGI59_001108"
     CDS             20395..21048
                     /gene="eutL"
                     /locus_tag="ACGI59_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HFG9738911.1"
                     /translation="MKNDVIRPNILGIKIISNISPEMAQKLELKSHHKSLGLITADCD
                     DVTYTALDEATKASEVDVVYAKSMYAGAANASTKLAGEVLGIIAGPSPAEVRSGLNAV
                     VDFLEYGATFISANDDDSIAYYAHCVSRTGTYLSEVAGIREGEALAYLVAPPLEAMYA
                     LDAAMKAADVKMCELFAPPTETNFGGALLTGSQSACKAACDAFAEAVKSVADNPTGF"
     gene            21059..21760
                     /locus_tag="ACGI59_001109"
     CDS             21059..21760
                     /locus_tag="ACGI59_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HFG9738912.1"
                     /translation="MDAIGLIEVIGYVAAIEASDACLKSANVNIVRVDKVGAGIVTLT
                     ICGDVGAVKAALEAGEIAASRVGTLRTSHIIPRIHNEVTNALFKTKEPKVCEVSEDKE
                     KNFEITLEIADNVSKNLSEKENVILDVNENSGDTENLYSDIDKTNKKIETVDDSLTEA
                     NEEKIENTNDKEVLEQVKIENSDFDRDDLSKNSVKKLKAMAKKLDSSITYKDLNVLKK
                     EELINLVKKLSRRDR"
     gene            21763..23232
                     /locus_tag="ACGI59_001110"
     CDS             21763..23232
                     /locus_tag="ACGI59_001110"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase (acetylating)"
                     /protein_id="HFG9738913.1"
                     /translation="MTLLDKDLVSIQEVRTLVRRSKVAQEKLSKMSQEQIDKIVKAVV
                     DAAFNNATKLAKMANEETGFGKWEDKVIKNTFASMGVYETLKNTKTIGIIGEDKEANT
                     IDIATPVGVVAGLIPSTNPTSTVIYKTIISLKAGNTIVFSPHPNAKNCILETVNIISD
                     AAIKAGAPEDSILCITNPSIVGTNELMKHKDTNLILATGGEAMVRAAYSSGTPAIGVG
                     PGNGPAFIDKSANVKLAIKRIIDSKTFDNGVICASEQSIVIEKSMEETVLKELKNQGA
                     YVLNEEESERLAKFILRANGTMNPQIVGKSVEVIANLADLTNVPKNTKVLVARENKVG
                     HNVAYSREKLTPILAFYVEENVEAIMNRCREILLNEGAGHTFSMHVNDEELVKRFALN
                     MPVSRILVNTPSALGGIGASTNLVPALTLGCGAIGGSSTSNNIGPLDLINVKRVAYGI
                     KEIEELGRDKSQVEKSMFDSMDKEELINLLVQKLVKELA"
     gene            23328..23615
                     /gene="eutM"
                     /locus_tag="ACGI59_001111"
     CDS             23328..23615
                     /gene="eutM"
                     /locus_tag="ACGI59_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HFG9738914.1"
                     /translation="MASANALGMIETKGLVGAIEAADAMVKAANVQLVGKEQVGGGLV
                     TVMVRGDVGAVKAATDAGAAAAERVGELISVHVIPRPHFEVDAILPKVSAE"
     gene            23742..24503
                     /locus_tag="ACGI59_001112"
     CDS             23742..24503
                     /locus_tag="ACGI59_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin adenosyltransferase"
                     /protein_id="HFG9738915.1"
                     /translation="MGVLTENEVKRKIGKNKNNITEFYIEKGQIITPSAKSYLAERSI
                     ELKYVDKLSDVNNEPKEVVKEVVKEKKYRYTTVFGAKLDEKPEHMTQLNGNLLVFKDH
                     KRIILRGKIDSLESEILKVQILCQKLDMPKLVKELQEVLDFVRNIMRCEVLDEKLGDF
                     KLLGLTPQELREQSHYPKKYFGIGHEFPSYEMGEVVVAINSIRSNTREVELAAYEAFK
                     GEYGNVHREDLIKALNRLSSVFWIMIYKVRTNKYK"
     gene            24516..25145
                     /gene="eutD"
                     /locus_tag="ACGI59_001113"
     CDS             24516..25145
                     /gene="eutD"
                     /locus_tag="ACGI59_001113"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization phosphate
                     acetyltransferase EutD"
                     /protein_id="HFG9738916.1"
                     /translation="MENLLNEIVEEVITRVKKEAFIEVEASGRHVHLSREDVDKLFGE
                     GYTLTKLKDLSQPGQYACKERVTITGPKGSIKNVVVLGPCRNETQVEISLTDGSTLGL
                     KAPIKQSGDLEGTLSIKISTQYGEVELDKGLMVAKRHIHMTHKDAEKFNVCDKEIVEA
                     KVMGQRPLTFDDVVIRVSDSFKTYMHIDYDEANACGYSKGTVAKIIKKA"
     gene            25187..25912
                     /locus_tag="ACGI59_001114"
     CDS             25187..25912
                     /locus_tag="ACGI59_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02536 family ethanolamine utilization
                     protein"
                     /protein_id="HFG9738917.1"
                     /translation="MNYDNLVDLITEEIYKKINSNEIKISNKPKAVIVFEQDNNKFNL
                     LKDEFEIVEFDKSIRECEIVIVSRLCMRGLSNIALGNSTSDEERFILKMIMKGKEVYV
                     LDEGIEYKKYKDTAPKALYKKFMSFEDEICKFGVEIIKDLNVITKNKLNIKKELESTR
                     GSKKEDTKVLDKDVLNLTSVESVKIDNEFSLDLRNKKLISEADLRKPTINGVKNILVN
                     KKSIITPLAVDFMRIHHLKLKKV"
     gene            25925..26197
                     /locus_tag="ACGI59_001115"
     CDS             25925..26197
                     /locus_tag="ACGI59_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HFG9738918.1"
                     /translation="MDIGKVVGNVWATRKDEKLCGQKLLVVKILKTKDTYKEGFMVAA
                     DNAGAGNGDLVLISKGSSARESIEKSHVPVDATIVGVVDSFEVFDE"
     gene            26190..26741
                     /locus_tag="ACGI59_001116"
     CDS             26190..26741
                     /locus_tag="ACGI59_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HFG9738919.1"
                     /translation="MNKSIGAIEFKSIAKGIEVSNEMIKKSSVDVLYLKSICPGKFLI
                     IVGGETSYINECVDYGIKLGEGYIVDNFVINAISQEILDGFKNKYQKLDSIVSIGVVE
                     SSKVCAGIKMLDKTLKSGDLVLVKLQLSFAIGGKLVYIVAGDLSSLEYALKESENVVR
                     EKEVIYKTVIPSVDSQIIKSLIK"
     gene            26753..27844
                     /gene="eutH"
                     /locus_tag="ACGI59_001117"
     CDS             26753..27844
                     /gene="eutH"
                     /locus_tag="ACGI59_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HFG9738920.1"
                     /translation="MSINEIIIYVMVVFMVLGAIDKCIGNKFGLGEQFEEGIMAMGSL
                     AVAMVGVICLAPVLADVLRPIVVPVFNILGADPAMFAGSLLANDMGGAPLALELAKDP
                     NAGLFGGLIVGAMMGPTIVFIIPVALGIIEKEDQKFLATGILAGIITIPIGAFVGGLV
                     AGFEVMMVLRNLVPIIIFAVVIALGLLKFENAMIKGFTYFGKGVVIVITLGLAAAIVE
                     ALTGIVVIPGMKPISEGIEIVGDIAIVLAGAFPLVFVITKVFNKPLMALGKVLGMNDV
                     SAAGLVASLANCIPMFGMMKDMDNRGKIINVAFSVSAAFVFGDHLGFTAGFNSNMITP
                     MIVAKLVGGITAVLLAMVIANKTLKKENV"
     gene            27846..28319
                     /locus_tag="ACGI59_001118"
     CDS             27846..28319
                     /locus_tag="ACGI59_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFG9738921.1"
                     /translation="MDISNIDKNLIETLVRQIIEEKISGTKDTVDFVRNKDISGITSI
                     KLPTVKVSESDRLDTGNPSDVVYTKDLFTLEESPRLGCGMMEMKETTFDWTLNYDEID
                     YVIDGTLDIIIDGRKVSASSGELIFIPKGSKIQFSVPDYARFIYVTYPADWASQN"
     gene            complement(28439..28534)
                     /locus_tag="ACGI59_001119"
     CDS             complement(28439..28534)
                     /locus_tag="ACGI59_001119"
                     /inference="COORDINATES: protein motif:HMM:NF012392.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFG9738922.1"
                     /translation="MGYPNVEHFIRLFNKKYNMSPSKYRKNTSKI"
     gene            28835..29356
                     /locus_tag="ACGI59_001120"
     CDS             28835..29356
                     /locus_tag="ACGI59_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFG9738923.1"
                     /translation="MEEIVKKEQASMNDIFLYLKAKSNSLYKFVILYNEFINKPKDYG
                     TGELINMVEVHMLTAIEENPGISPTQLAKIWNRTKGAISQTISKLEKKGYITKEKKEG
                     NAKTILLYPTTSGVELSKAHKLYDTIDVTRTFEYLIKECTLEEIDSFYKVIDLFINLL
                     EESPQENNDSEKV"
     gene            29735..30910
                     /locus_tag="ACGI59_001121"
     CDS             29735..30910
                     /locus_tag="ACGI59_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFG9738924.1"
                     /translation="MNLKQLSEKYEEYVIQMRREFHKYPELSFKEIRTTQRIREELQK
                     IGVPFVSIEPNIVIATIQGKNEGKTIAIRGDIDALPMQEDSDVEYKSEYDGIMHSCGH
                     DAHGAMLLGLAHMLNDIRKDLKGRVLLVFQAAEEVGGGHKEIIQYFKENGGPDRLIAT
                     HVWSDIEAGKISVEPGPRMAGGSGWRIDITGRGGHGSRPDQSIDPIKPACDIVLKVSS
                     IPVNHISVLEQCVVHACAIHGGTTIGNVIPNNAEVIGGYRYFTEESGKKVFDIIKQMS
                     NGVGQVYGVDVKVTHTGGIGPVINDEEAVKMAKEIVSNIDGLELDSYEPICASDCYGE
                     ILKEYSGFYCYLGVGNKEKGIIYAQHHPKYNIDEDTLKLGCEFMAKYAVEFLNKNNN"
     gene            30945..32483
                     /locus_tag="ACGI59_001122"
     CDS             30945..32483
                     /locus_tag="ACGI59_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HFG9738925.1"
                     /translation="MQGALKENKKKSGGGFLGWIEKVGNKMPHPLALFTYITLIVVAL
                     SAIASFLGFSATSPATGKLIKVMNLISAKGLVLFMQEFVKNFQNFPVLGVVLVMAVAT
                     GVCEKTGFFSTAIKMSLSKVKGNAVVFIIAFIGVFANQAGDAAFVLVPTLAGAIFYGL
                     NRNPLAGIFCGFASVGGGFATAVIPGGWDVTLTPITVSAAQTIQPAFDMTLLASYYAL
                     FVSAFIVATVSTIVTVKFIEPKLGKYTGKPEGIDDNQGFEVTKEQAKAAKLAGFTVLA
                     YVALLIALCIPANSFLRSPEGSLIFGAPLMSCLQFFIVILFFLPGIVYGMRVGKIKTV
                     KDLNDILCQSMAAMAPFIVLAIVIGQFLTLFSVSNLAQVLAIKGGALLNSLPLPPQAI
                     ILLFLVLVAFINIFVISGSTKWMIFGPVFVPMLMQLNIHPAFTQFVYRLGDSVTNHLS
                     PLNAFFIILLATVQKYDKNAGMGTIFSAMIPYTIGYFVVLAVLVVVWMTIGIPVGIGG
                     QVWL"
     gene            32515..33717
                     /locus_tag="ACGI59_001123"
     CDS             32515..33717
                     /locus_tag="ACGI59_001123"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFG9738926.1"
                     /translation="METTEIRYNFDEVIDRSGTNCLKYDLKSQFMPESPEDALPLWVA
                     DMDFACAPEIINAMHERIDRKIFGYSSQQTDDYYEAVCGWFKRRFDWKIEKENIFFSP
                     GVVPALGFFTQILTQKGDGIIIEKPVYYPFEDKITNNERKVVNSPLKFEDGHYSMDYD
                     GFEELAKDENNKVFILCSPHNPVGRVWKKEELRKIVDICKKYDLWIISDEIHCDIIRK
                     GEKHIPLEVACPDYKDRIITCTAPSKSFNLAGMQLSNIVINNKELQKKWMLETDSKLA
                     IFLPNPFAIVATKVAYNDCEDWMNQVNEYIDGNIKYVEDFVNEYMPKVKIIKSQGTYL
                     VWLDFRGYGLDAKQLEYVMLNKAKVLLDEGYIFGEEGAGFERINVASPRTIIEECMNR
                     IKCAFEGL"
     gene            complement(33884..34483)
                     /locus_tag="ACGI59_001124"
     CDS             complement(33884..34483)
                     /locus_tag="ACGI59_001124"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9738927.1"
                     /translation="MNDNIVEVKNINMNFYTKEGELEVLKDVNFNLKEGEILTLLGPS
                     GSGKSTILNILTNLLKPTSGDIKITGNIGYMFQKDNLLEWRNIMDNITIGLEIQGKKD
                     KKSLDRVESLLKTYGLWDFRSMYPKELSGGMRQRVALIRTLSVNPDILLLDEPFSALD
                     YQTRLLVCDDVYSIIKNENKSTILVTHDIGEARCIKVQL"
     gene            34653..36011
                     /locus_tag="ACGI59_001125"
     CDS             34653..36011
                     /locus_tag="ACGI59_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9738928.1"
                     /translation="MSNIITNLIESIFTNPITILFLLAITVYSVFKIIENNRVYSFVS
                     SKLDEINRDYKTSEFYKKVRDDYYTYSKENPYADVNITSFIEEVVSDLKHNNLPLLEK
                     IRSIKNSSSISILLGVLGTFVGLSTMLLCVDTKDIINSLPSTISSMQTAFTTSIFGVV
                     FSLIIGYFTKIKDCEHVLIQIMLKSENLLTSEITHFKSERMDLKVEEVKNTIKQISKS
                     IEAIEKFDKISKDLNDFNDEFISGIEALKSLLEGSQSSIKTFDQSVRKLDKQFNILNI
                     KFVKLFDKYDNQDNINKEILFDIKESSKNIYNATESQFKIRDYIKNINAGFALYERSA
                     QDLLTKLMTHENKISQNQKILLDEKFTLDDSIKNLSSIIENFSNDLQVKLDMMFENSL
                     DIQDKLDVMFNNSFMNDEVPLDSEELFKDDVNNVFNPFSEEIYEIEDKEIKVIGEDEL
                     NE"
     gene            36004..36636
                     /locus_tag="ACGI59_001126"
     CDS             36004..36636
                     /locus_tag="ACGI59_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFG9738929.1"
                     /translation="MNNKYRRTVNREFEKSSFWPTFTDLLSTVLMVVILILFSSESIS
                     GSVEQDLAKNVNASVEETFKKSGIPVKVDKSNGQVTFGEKTMFDVDSDVLKPEAKEML
                     KMFVPKYIETIYKDYGDYISKIVIEGHTDDVGSYIYNLDLSQRRAYSVAKFIVGDEIG
                     DYKYKDKVTKHIIAIGRSKAELIKNGDNSVNRDASRRVELKYEININQNK"
     gene            36965..38122
                     /locus_tag="ACGI59_001127"
     CDS             36965..38122
                     /locus_tag="ACGI59_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HFG9738930.1"
                     /translation="MIEISIKNKKNSKIKTLILLLIVSISIYLTGCTTSDNGNNQSNN
                     NDSLSSSISSDKNVKIHFIDTGNSDAILIQDGKTFTLIDGGDNDDEGLMVDYLNNQGV
                     KDIKYLIATHSHADHLGGLDSVVKNFNIENVFVSNGSAETKSYRDFINALANKGLSPS
                     VPLENNKFYLEDSYFEVLNTNGGDTTNEQSLVLVYTNGNDKVLFTGDAEEGTEKEILP
                     KLEKVDLLKVAHHGSRSSSSQEFLDKVNPEYAVLLVGKGNSYGHPHQETMNKLKKMGV
                     KVHRSDECSDIIFESTGDGVFTSCKDGSYNKGVREDGNSGKSNSNTSKNDSFKNKQNT
                     SNSEEIVYFTPKGKSYHSTKNCSGLSRSKKILSGTIAESKKNDPCDICYGK"
     gene            complement(38269..38904)
                     /gene="lexA"
                     /locus_tag="ACGI59_001128"
     CDS             complement(38269..38904)
                     /gene="lexA"
                     /locus_tag="ACGI59_001128"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFG9738931.1"
                     /translation="MYLDLTEKQVLILEFIKSQIILKGYPPAVREICTAVGLRSTSTV
                     HSHLNKLEKLGYIRKDPTKPRAIEVLERSKVNDVSGANQEIIELPLVGQITAGEPILA
                     QQNIEEYIPFPASLVKGSNNFVLRVKGESMINAGILDEDYVVVDKKNTALNSQIVVAL
                     INGESATVKRFFKEGNLIRLQPENDFMEPIMLNDSEVEIVGIVTGVFRVIK"
     gene            complement(39008..>40122)
                     /locus_tag="ACGI59_001129"
     CDS             complement(39008..>40122)
                     /locus_tag="ACGI59_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFG9738932.1"
                     /translation="KIIKVHNKSDKPDNIVIKENELIEKCLLLENKLPVFMKDYFIYL
                     KGSVAVSTRLAYLEDINFFCSYLIETKELTNADCVKDITEDDFNTIKSRDINLFLGDY
                     CSRYYKNTEKNTLIFENNNRALARKKSSISTLFKFLYRNSQIDNNITDGFNPIKLPKP
                     QPDAIKRLEIDEVAKMLESVETGEGLTEKEKVYWRKTKLRDKAILALFVTYGLRLNEL
                     RELNISSFNFSRGEFKIYRKRGKEVLMPINHTCEHVIKDYLQNERTRDELLNDEEKDA
                     LFLSLQNKRITAKAIRTLVKKYTSIPLDTTRDNGYSPHKLRATAATSLIQTGFSIYDV
                     QNLLDHDNVTTTQLYAAHKKNVKRDIVKNFEWIEDD"
BASE COUNT        15508 a         4980 c         6550 g        13084 t
ORIGIN      
        1 acatcagttc ttacattaac attgcctgct tttagatttt ctccaatagt aagacctttt
       61 tcttttagaa catattcaaa agtcattagt ggaactccac cttttctacc acctaatatt
      121 tcggttcctt ttaaatcatt gtaagagaaa ttaggcattt ttttcctagc aactagaaaa
      181 ctaccatctc tttttgtcaa ttgagcaaag ttcactgcat aatcgttttt gttttgatta
      241 tatacatata tagatgcttc aggacccatt aaacctacgt ttgcttcacc tgaaatcaaa
      301 gctgccatag ttttatctgc accttgggta tttataaggt caatctttat accttcatct
      361 tcaaagaatc ctttcgtaat agcagcatac tgtggagcat aaaaaacaga gtgagtaact
      421 tctgctatag ttactttatc cagattttta gacgaagaag attcaccttt tttgtcatta
      481 gagttaaaat cacatccagt tagacatatt gtacttacaa tgatagagct tgctattgcg
      541 actatttttt taaacataat aaatacctcc cataagacat acgaatagta tatagtatta
      601 aaaatttatt gtagttgtta caaaaatgta tctatgctta taatatttct acaaaaactt
      661 aataataatt ttcagcttta tgtttagttt tttcagtaat gtgtaatatt ggataaatta
      721 tattgattat ttataaacta agtaataaaa ttaaaataat atcacttttt tgtctattat
      781 atataaggaa aaatttttta ataatataaa tcatattcat ttatattatt aaaaaatttg
      841 tttatagaaa cttttattat tttatataaa agtgataaaa acttatattt tgtatttaat
      901 agagtgttgc taagtgaata tatttaaaat ggaggaaact ttatgaaaca aaataaatat
      961 gatgatgata gattttttaa taaatacagt aaaatggaac gttctacaaa tggattagca
     1021 ggtgctggag agtggcatgt tttaaaaaaa atgttacctg atttcaaagg aaaaagagtt
     1081 ttagatttag gatgtggatt tggatggcat tgtcaatatg cagttgagaa tggagcaatt
     1141 tctgctgttg gagttgatat ttcagaaaaa atgttaaaag aagctagaaa caaaacgcaa
     1201 cttgataata taaattacat atgtatgcct attgaagata ttaattttcc aaaagatagt
     1261 tttgatgtag ttattagttc cttggcattt cattatattc aatcatttga agatatatgt
     1321 aagaatataa gtaattgtct ttcaaataaa ggtaattttg tattttctgt tgaacatcct
     1381 atatttactg catatggaag tcaagagtgg tactgtgata aagatggcac taatctacac
     1441 tggccagtag atggatattt tgaagaagga gtacgtaagt caaattttct aggagaagag
     1501 gttataaaat atcataaaac attaacaact tatttaaata cgttaatgaa aacaggattt
     1561 gaaatattag aaattattga acctcaacca gaagaaaaac tgttagatac aattcctgat
     1621 atgaaagatg agctacgtag acctatgatg ttacttgtat ctgcaaaaaa aagataagta
     1681 aatataaata atcattattg tagaaaaaaa gttgtagaaa ataattacac taatggagat
     1741 gattatacca gtggtattaa ttacaccagt agtaaggagg caaaagcatt cgttgctttt
     1801 gccatttttt aagttgcaat tatacttcta cgtagtatat ttcgtttcca ttttcttcta
     1861 taagcttttt catatcttca aattttaaaa atacagtagc agtattatca tttggatgta
     1921 tacctatttt atccattttt aataaatctt tatcaaatac aacttttaca acagcctctt
     1981 tatcatttaa tataccaaaa ggagtcacag ctcctttagt aagtttcaag tatttttgaa
     2041 gtctttcttc cgatgcaaaa ctaagaggtt tactatcaat ttgttgacga atatctttta
     2101 aatctgcttt tttgtcttta cctaatacaa ctaaaaaatg ttcttttcct ttagcatcac
     2161 gaagaaatag gttttttaca acaaattcca tatgtaatgt ttttaattct tccatttctt
     2221 caattgtata aactggtgga tgatttacag cctcataagg tatgcttaac tcatctaatt
     2281 ttttatatac ttcctgttgt tgattgttca tgatttcctc ccattagaaa tttattttat
     2341 atataaataa tattatcaca gaaaacttta aaagaaaacg ttttatataa tatagacaat
     2401 agattatatt tttataaaga agcaagaata ctcaataaaa cttaaagaaa ttaaacaatt
     2461 tctaaaaata ccttgaaaat agaaccttat ttgccattta taaattatat aaaaagggat
     2521 tatataaagt catatatgaa aaaactatat tatactatct tttctttttg gaataaacta
     2581 tactttaaat atagatattt aatttaaata aattttacag tattaatagt aaattatctt
     2641 agaaaggaat gtgctttata tgtctacaga caaagttgtt ggaatcatag atgaagaaac
     2701 ggcagaactt gcaggaattg aatatactgg taaaatttat gcttcttctg gtgttataaa
     2761 acatataaag aaaaaacata gatgtcaact gagtaaggat atttttaatg atataataga
     2821 cactataaaa atggtattaa agtcacctga atatatagga agtcatccta aaaaacctgg
     2881 aaaaagtgtg gagtttataa aaaaaattga tgattatatt ttagtagcta cagaattaga
     2941 tacaaaaaaa ggatatttat atgtatcttc aatgtatgct ataaaagaag caaaattaga
     3001 aagtagagta aacagtggaa gagtaaaaca atataatggt taacataata ttattaattt
     3061 tccctaaaat attgaaaaat attattatat aagttataat attaatatac acgtagatat
     3121 ttattagtta gatttgaggt tggaaaaggc acccaatacg ctactataga gtagttaata
     3181 gagatgcagg atacgccgcc ctgccaaatc taattaaatt aaaaagagag gattaattcc
     3241 tctcttattg tttttttgag atactaattt aatctataat agaaagattg aaggtcgttt
     3301 atacaaaaaa attttcagtt atatacatta aactaattaa ataagatatt tataatctat
     3361 atgtgataca tattatatat taataaaaga aaaatatata gaaaatcctt ataaaaattt
     3421 aaattgtaat gaataggaag taattaaaaa gaatttttat tgatttttaa aaatttagtt
     3481 agaaactata tcattatggt aaaaaatgta ttataatgtt tagaaatatt ataataagga
     3541 gcgattaaac atgaatctat cgaagaaaat agaagataaa agaaaagcta aacagagagc
     3601 tgaaaaagtt aagaaagcta aaattgctac agctggtgtg gtattaggag ctgttactgg
     3661 tgcagtgagt ggagtacttt tggctcctaa atctggaaaa gaaacaagag aggatataaa
     3721 agatgcttca caacaaattg ctgaaaagat aaatatgaaa actgtagatg taaaaggcaa
     3781 agtatctgag aagctagagg ataaaaaagg aaactttata gaatcaaaga aaaaaataaa
     3841 aaaatatata gataacaaaa agagtgttga aaattctagt gaggaagata aaataataga
     3901 tatagttgaa ccagttagtg ttactgagga ataatatgga caatacaata acgattaaca
     3961 taagtataat atatgtatta ttagctatac ttgcttgtat tgctttagtt tatttaatta
     4021 ttgtacttgt taaactacat aagattttaa ataatgttgg aactatgtta aatgaaaata
     4081 ctcaaaatat aaatacaact ttaaataatc ttcctaatgt tgtagaaact tttggagaag
     4141 taggagaaaa tatgaaagat gtaacagaag tagttactga agttacagca gattttcttg
     4201 ttactaaaga cagtgtcaaa tcaaatatgg atgttatagt tgatatattg actataatta
     4261 aaaacatttt tctagataaa aaataataag tttaattaaa ataagcctat aagatataat
     4321 ttaaacgacc ttaaaatagc atttataagg tcgtttttta ttagcttgat ttgacactat
     4381 gtgtttttaa taattggtaa tattcctctt tatcatttga acatatatat ctatgcttgt
     4441 tgtttcaatt acatccataa gaacatcttt acattcaagc tcaaaatctt ccattccttc
     4501 taaatattca tcatcttcat caaaaaagat ataagaatta tatagccatt cttcaaattt
     4561 ttcttcaagt atatcccaat acgaactcca aaataatcca caacctgata tataagatgc
     4621 tctactttga tttatataaa cctcctctag aagcttttgt aatctactgc tataaagaaa
     4681 atctgctctt ccactgcttg atattaattc atttaaatct tctattgaca ttaattcaac
     4741 tttaccactt ttatcataag gagtataatt ttcatcaacc catttatgtg gtttgttttc
     4801 tctgcatact gttgctatca actctacaac ttttgttctt atcatttttt tatcctaact
     4861 tatttatttt ttataaaata atcatgattt tataatttat ctatttcttg taatgctaat
     4921 ttaacagttt catatctatc agcaccattt attactacat caccttttac aagtttatta
     4981 gcttctccac ctacaacata aaccttttta cctaaacctt gcttataact agacgcttca
     5041 attattaaag aatctttcaa ttgccaatgt aaaatctgag cagccacttt atcagcatca
     5101 cctgtataaa cgattacatt ttcatacact ttattttccc ctccctcttg agaatttgat
     5161 atagatttgt ttagtatacc ttctgctatc aatttagcaa ctttatcttt attattaaca
     5221 taataatctg tatctgcttt actatctaca aaacatactt ctattaatat tgctggtgca
     5281 atagtatgat taagccaata aagacctcta acatcagact ttgcgcccct atctttgaat
     5341 actgtagcta acctagtatt cactctttct gcataggttt tgccattatt agtcttatat
     5401 attgtttctg tccctactgg agttgaagtt gtggcatttg aattaaaatg aatctgaaca
     5461 gccaaatcag tttcctgttt atttgctata gcacattggt ctgctaaatg attacttgat
     5521 tcatcaactc ttcctgtata aacagtagct ccacctattt taagccattt aacaattaag
     5581 tcattcagta ttcttgtttc tttgctttcg tttatatacc caacagctcc tgtacctttt
     5641 ccaattaaag tgtgccctgg tattattact actttcaaac taacatctcc tttacaatta
     5701 aatttaactt ctatttaata tatgcataac ctagaatttt gtttcttttt atatcttata
     5761 cttttattat aacccatttt gtaaaaatat agaatattaa gtaaataatg ttaaaattat
     5821 aattttgtat ataagacatt acttgattgc aataatttta tagcatattc atctattcta
     5881 tatccagttc tataatatat atgtttaatt ccagcttgaa ttatagcctt agtacaattt
     5941 aagcaaggaa agtgagttac ataaatagag caatttttaa ctgaaatacc ttcttttgca
     6001 caataataaa gggcattttg ttcagcgtga atggttctaa tacaatgtcc atctctcatt
     6061 tcatgaccaa cttcactaca atgcttatct ccagaaatac taccattata tccagtagac
     6121 actattctgt tttctgaatt gacaattata gctcctacat aagctctatc acaagttcct
     6181 ctttcagcta ctgtttcgca cagtctcata aaatattctt gccatgagca tctattttca
     6241 agttttttat tcaaattata tacctccaaa acttatatct aatatataag atttatacct
     6301 taaaaataat attaaaaaca aatttatacg ataaataaaa tttattaata ctgtttttag
     6361 tctaaattat ttcattttaa attaaagtta caaggaatct aaaattatat atatctttct
     6421 tcgtaatatt aatgttacaa tataaaaaac tgtctcaata aaatagagac agttttacag
     6481 tcaaagttta taatacaagt tcatattttt ctttaaattc ttttacagct tcgggccatg
     6541 ccttttgttc tgtaatacct ttttctttag ctattttaat tgcaatctct cttatcattg
     6601 cagcgccttc taatattccc atttttaatc tcccttcaaa tatctttttg tatagaatat
     6661 aaatagtgct aagatttatt acatgctgca tatacaatat ctatacaaca cataacttgt
     6721 aaattccttt tattttcata cgtctttaac cgacatataa ttatattcaa taagttttaa
     6781 aattatgaac tcaattataa ttatttttat ttttttagaa tctaaacact aaaaattgta
     6841 ggtaaatttg tcttatgcct ttataaatat ttctacagtt ttaattactg gacatatcta
     6901 tttttattat aaaaagcgat taaaaatcga gaatatccgc ataaaaactg tatataagca
     6961 aaacggttaa aggaaaaaga agaaatttac ttgaatgtta aaatgcgttg cttgaagcaa
     7021 tgggcagttt tgcctataat atcggtaaca actgaaagaa aggtggttag ccctaatgtt
     7081 aagcgaaaac attaaaacaa tcaggaagtc aaaaggactt tcacaacaag aacttgctat
     7141 aaaactgaat gtagtgcgac aaacaatatc taaatgggaa caaggactgt cagttcccga
     7201 ttctgatatg ttgatttcca tatcagaggt gcttgaaacg cccgtaagta ctttacttgg
     7261 agaaactatt gttgagtcgg aagttgataa cttaaaagtg atttctgaaa aattagagat
     7321 tataaacttg caacttgcac aaagaaaaaa catgagaaga aaaatgtttc attggttatt
     7381 tattttatta tgtgcagtca tattaatagt ttttgtggtc ttagggggac taaatagtcc
     7441 ttacttggat tggaattata ccgaccctga aaccgctgtt tttggagtag cttttcatgc
     7501 ttttgagtgg ctatttgtca gattagtacc gattgtcctt ataggagcaa ttattggaat
     7561 ctttttgaca cgtaagaaaa tatgaagtaa ctctgctttt tataatacag caagcgttcc
     7621 ttaaaagcgt cattctacat ggcaatcgag gacgactatg taaggaagaa cctttttgat
     7681 ttccaactaa gcgaagttat tgaggacgac agtaagacca aaaccgcatt aagtaaagta
     7741 caggaagcaa gactgctgga cttatactct catgcgtcag atgcaggaaa tgcgaggtct
     7801 ggtagcatga tttacaacga tttgtacaac gactgctagt agaaataata tggagataag
     7861 tattatctac aaagtcagaa aagaaatgac ttgataattt actcatgtta taattattga
     7921 tgtgaaatta ataaatcgaa attccttttt tggatttaat aatactgagt aaggaaaaag
     7981 tgaattactt ttgcaaggag gtgttaatat caggcaagta aagtcttttg tttttccagt
     8041 tatatcctta atattcttag accaaattag caaagttctt ataggattat tcttaatgga
     8101 ctttgaaatt gatataattg ggaaattttt aagattcaat cctgttcaaa atacaaatct
     8161 ctcttatggg ggaaacttta ttggtattct atctaattta tgggtattgg tattgtttaa
     8221 cattttagtt atattagtta ttatatctgg atatgctttt tataaatcaa aaaatgaaca
     8281 aacaagctat tcagtaaaag taattatgtc ttgtggactt gctggtacaa tatgtagctt
     8341 gatagataaa ttattttggg gaggaagttt agattttttg cagataccaa gcttttttat
     8401 tttcgactta aaagactgct accttactgt tgcagaaata atatttgttg tcataggaat
     8461 tttgcataat agagaaatat caatgaaaga atacatatat ttttgctatc gtcagtttaa
     8521 aaaataaatt tagaagataa atttaagttt caaggtaaac ttatttaatc acagcaaata
     8581 gttggcttac ttaagctttt gggctaaact gtgagcacta attaagtctc tgatatctaa
     8641 caaacggagc acaaaaataa aaattaaata taaagcacag ttaattttga ttaaaaagtt
     8701 gactgtcttt tcttttgtag aaatttaagc ataggtaata aagaaatgac tgcatttaag
     8761 cattatgtat ataattggag gcaataaaaa gccagcaaag cccataaaat agagctgtgc
     8821 tagcaacatg atgaattata agaagataat caaaaactta cgctttattt taatttaaaa
     8881 aaatgattac tgtaaagaaa tcatttttta ttataatcac tgatacatca aatattctaa
     8941 ataaaatttt tatttatctg gaattgtata ttttttctat aatattaact acagcgtaca
     9001 ttgcccaagc acagatagcc aatacaaata ctcccatcat tactaaatct aatttaaata
     9061 cttgagaacc atatactatc aagtagccta ttccatatct tgaaactaaa aattctccaa
     9121 ctattacacc tacccatgcc ataccaatat taattttagt taaatttatt aaatttcctg
     9181 tgtttgcagg taatatgagt ttaaataata tttgagattt agtagctcca aaacttttta
     9241 acatcttaat tttttcttca tctatattca taaaataatt ataagctgaa agtatagtaa
     9301 caacaacaga aatcgttaca gctgttacga cgattccttc tattccagca cctacccaaa
     9361 ctataattat aggagctaaa gcagtcttag gaagagcatt taaaactacc aaaaatgggt
     9421 ctaatatttt tgataatttt tctgaccacc aaagagcaat tgctaccaaa atacctaata
     9481 cagtacctat aacaagacca agaacagttt catatacaga tattccaata tgtttaaata
     9541 attgaccact aaatgcatat tgaataaata aattgtaaat atcacttggt ttgctaaata
     9601 gaaatgtatt tataacattt aagtcagcta aaagttccca gattacaata aaaccaatta
     9661 aaattaatag ctggtagaat aatacatttc ttttttctct ttttacacct ttaacatagt
     9721 ttatatatcc ttcagatctt ttattaagcc tcatgtgtat ctagctcctt ccataatatg
     9781 tcgaaatact tttgaaaatc aggaacttta cgtgttttga gaggcgtttt tgttccatcc
     9841 atgttaagtt caatatcgta aatatttttt atggtagctg gtcttttaga tagtattgca
     9901 accttatctg atatgcttat tgcttctgct atagctaaaa aagtgcaact aattgcttaa
     9961 agaaacagaa ttatcaactt cagcacccat ataaacaata ttaagatttc ctaaactgct
    10021 atcccaaacg atactattaa taatagtaga taataacaat ctcttatcat ctaaactggc
    10081 attatcaact tcatcattaa atctcttcaa attactaaca ataatatcaa tattaatact
    10141 gtcactacta atattgatgt tactagattc caaagtatta atcttatcct ttaaaatact
    10201 aatctcatta tttaggctct caatctggga aactatataa tcagaagcag tactaccagt
    10261 attttttgca agctctataa ctaaattttt aataagtctt tccttatcta aaatttgatt
    10321 tttaagtaaa ttagtctcag atttaaaaat attaaaaaca ttatcagtct taatgttatt
    10381 actattgaat gcacttataa tagactcttc tttacaattc ttaattttat ctataactaa
    10441 actttcaact aatggaccat taagattttt acagttgcaa ctatcagacc caagtgtttt
    10501 tttagtacca caaatataat aatactttaa atccttgtca gtttttttgc ctttataggt
    10561 aattctcata ttagaaccac atttaccaca ttttataaga ccagataaaa gagcagtttt
    10621 tccagttgat atccttggag ttttatcacg atttttatct aattgatttt gtacttcaag
    10681 ccatatttca ggttctatga ctccatggtg ttttgcaatt gctgctattg agttagatgt
    10741 atttttagca taggtaagga ttccacattt tctatctgga gtacctacaa catccatacc
    10801 actattttta aggtaatcta aaatttcatc attagctttt acatatgctg gatttctaag
    10861 tattctagat aaagaactag ggtcaaattt gccaccacga ggacccctta ttccattatt
    10921 atagacatat ttatataact tagataagga cctaagttct aaatatttat tgtatattag
    10981 ctttacaatc tccatttgtt catcatttac ttgaagctta tacatacttc tttcttttag
    11041 attttcatct aagaatgtga ttttttcact gttgaatcca tgaggtgtat tgcctccaag
    11101 ccatctacca ctacgggcta attcatacat attgtctttt atacgttctg ctattgtttc
    11161 tctttctaat tgagcaaata cacttgatat aaacatcata gctcttccca ttggagttga
    11221 agtatcaaat tgttctttga tagatataaa tgatatgctt agttcattta atttttctat
    11281 taaagtagag aagtcagata catttctcga tatcctatct aatctataac aaactaggta
    11341 actaaatttc ttatctttgg catcctgcat cattttctta aattgtggtc tatctatatt
    11401 gctaccacta aatccctcat cttcatatat caaaaaatct tttacaccaa tagactttgc
    11461 ataatcaata cataattgta tttgattttc tatagaatca ccttttccag taaatcttga
    11521 ttttcttgaa taaattgcag ctttcaaaat taaaaccccc tataagttag gatatatatt
    11581 tgaaactatt tatctacaac cttttaagta aacttattag aatgaattta ttaattaggt
    11641 aaatttatat aattctactt tttataatat atgactattt attttatttc tttactattc
    11701 aagttctaaa tcaataataa attttgatat ttaattgtat caggacaaat ctctaatttc
    11761 atagattatt aatataaaat cataggttag tgtgaaaaag ttgaaattta aatattttaa
    11821 aagataattt ttttgaacta ataggtataa caaaaattta tagggggatt gcatatggaa
    11881 atcaatgtta tagaaatttt ccctaaagat aaagctaaac ttaataaaat agaaatggat
    11941 aaagctagtt ggtttgtaaa tataataagt aaaaaatatc ctaaagaagc tttaaatgaa
    12001 gcatttagta ctttagaaaa agaattaaat ataagtaaag ctaatacata agctttgatt
    12061 attgatttta tatactattg gttttatacg ctatgacttg acattatatt aatcaaatag
    12121 tatattttta aatatagcac aatgaagtgt taaaagagca atggagctcc atagataaag
    12181 tctatggagc ttttttgcgt atttaactta aggaggatag gactttgaat aactttgaga
    12241 ttaaaactag aataaaattt ggagaaggct cactaaaagc tttgcaagaa ttaaaaaaca
    12301 aaaatgtact aataataaca gaccctttta tggtgaaatc aaaaacaata gataaaataa
    12361 catctaattt aataaactca acatatcaag tttttgaaga aatagtacca gacccaccaa
    12421 tagaagtagt agtagcagga atagaagcat ttaaatctat aaatccagac gtaataatag
    12481 cattaggtgg tggttctgca atagatgcag caaaatctat aatggacttt tctaaaaaaa
    12541 tattaaaaat taacgaagta gagtttatag caataccaac aacaagtgga acaggttcag
    12601 aagtaacatc atttgctgta ataacagata gtcaaaaaaa atgtaagtat ccattagtga
    12661 gtgatgattt acttccagat atggcaatac tagacccaga acttgtaaaa actgtaccaa
    12721 attttataac agcagataca gggatggatg tattgacaca tgctatagaa gcttacgtat
    12781 ctacaaattc taatgacttt tcagatgcac tagctgaaaa agctataaag ttagtattta
    12841 aatacttaat aaaagcatat aaagatggaa atgacataga agctagagaa aaaatgcaca
    12901 atgcatcttg tttagcaggt atggcattta accaagcatc acttggcata aatcattcta
    12961 ttgcacatgt attaggtgga aaattccata taccacatgg aagaacaaac tctatattac
    13021 ttcctcatgt aatagaatat aattcatcaa tagtaggata tactaacaca acttactctg
    13081 ctacagctaa aaaatatgca caaatagcag aattagtagg acttgggagt gctaatataa
    13141 gaatatctgt tagaaattta gtaaatgaaa taaagaaact acaaaaagaa atgaatatgc
    13201 caactaaatt gacacaatgc aaattaagtt tagaagatat aactaatgaa gaaagagaaa
    13261 tagcaaaact tgctataaaa gatacttgta cattaactaa tcctaaggta ccaactgaag
    13321 atgatataat aaacatatta aagaacatta aataactaca ttttattaca tgtaaatata
    13381 atattataca aatagggaaa atgtttttaa aaatataaat tatatattga caaatataaa
    13441 acataaaact ataatgaaag tatcacaaag gcgtgatact ttagtagaaa gcaatggagc
    13501 ttcttaggaa tattaatatt attcttatgg agctcctttt attttaatca aatagctttt
    13561 taataaacta tattcaaaga aagcagggaa taaatttgac tgaagaatct aagcaaagag
    13621 ttattcaaga atatgttcct ggaaaacagg ttacattagc acatatcata gctaacccta
    13681 atgaagatat atataagaag ttaggattag tacttgataa aaaagatgca ataggaatac
    13741 ttacaataac accaagtgaa gcgtcaataa tagcagcaga tgttgctaca aaggcttcaa
    13801 atgtatcttt aggttttata gatagattta gtggttcagt agtaatatca ggtgatgtaa
    13861 gttctgtaga atcagcgtta aatgatgttt tagaagtctt aggaaatatg ttaaacttct
    13921 catctacaaa aatcacaagg acataataat acaatgaaga atataatatt tatgggaaag
    13981 acaggtagtg gtaaaactac cttatgtcaa aaactagacc agttagaatt aaagtataaa
    14041 aaaactcaat cagtggaact ttataataat gcaatagaca ctcctggtga gtatatggaa
    14101 aatagaaacc tatataatgc cttaataaca acatcagcag atgctaaaat tatagcaata
    14161 gtgtatgatg ccacacaaga agaaaattat atagcaccag gttttgccag tatgttttgc
    14221 aaagacgtaa taggaattat aactaaaata aataaagcta ctgaaaacga agtcaatata
    14281 gcttatgaaa gactatcact agcaggagca agtcaaatct ttaaaataga cacagttgat
    14341 aatattggac ttgaaaaatt atttaaatat ctagaaaaat ataaaaatat agatgaagtt
    14401 taaggggaaa tattttttta ttcaaaaata ttattaaagt atgataaaat aaaaaaatgt
    14461 ataaactatt ttaagaagat ttaatcttta agcgaggtag gaacatgggg aaaaaaatac
    14521 tattggttga tgatgaacca ctgttaagga tggatacaaa agatatatta gaaagtcatg
    14581 gctatgaaat aataggcgaa gcaagtgatg gatttgaagc tgttgaaatg tgtaaaaaac
    14641 acaaacctga tttagttata atggatatag agatgccaat acttgatggt ataaaggcag
    14701 gcaaaataat atgtaaagaa aatatagctg gtggagtact tttattaact tcatttgaag
    14761 ataaagaata cgtagaaaag gctaaaagta taggtgctta tggatattta gtaaaaacag
    14821 caagtgaaaa atcacttatc ccaaccatag aaatgtgttt aaataaggta aatgaatttg
    14881 aaaaaatgaa aaaagattta gaaaaagtaa attctaaatt gaatgaaaga aaattagtag
    14941 aaagagcaaa aggtatactt ataagagagt ttaagatttc tgaggatgaa gcatacacta
    15001 gaataagaaa attaagtatg gataaaagaa ctacaatgat ggaaattgcc aaaatgatta
    15061 taataggata cgatgactaa tacgataaaa aaaatatcaa ctgcacaaac atacttaaat
    15121 aataacgaaa ttgaaaaaat tatagaagta tcaaaatcct tagatttaat ggcttctttt
    15181 tatgaagctg atgtttttat agatgtacta agtaaatttg atgatgaagc aattgttgtg
    15241 gcacatggta gacctaaaga aaagtcaatg tactgtgaaa atgttatagg taaaaaggct
    15301 ttaattgaaa atgaaccagg agttataaag tgcttaaaaa caggagaaag agtaagagat
    15361 attaaagctt taacacagga atataaactg gtcaaacaaa atattcagcc aataacacta
    15421 gatgacaaag ttttagcagt actaatagtt gaaaaagata taagtagtga agttaagaat
    15481 gagtttaatg ctagtgaaga cgaagaaaat aaaaatttac atcagtttat aaaacttatt
    15541 ggaaataatg actttttagt tgataattta aattcagcca tattaatatt tgataaaaat
    15601 ggaaaattaa aaattaaaaa tcgtattgca actgagatat acaaaaatct tgggtttgga
    15661 tatgatatac aagatatgca ttacaatgat ttagatatag aaaccaacac ctttgaaaat
    15721 atattaatgg atgaaaatta tgatgagaca aatgaagttt caataggtaa tatgtttttt
    15781 aaaataaaga ccatatacat caaagatgat gaatttaagg tttgcaaaat agtacaagat
    15841 ataagtgatt taaagagaaa agaagaagaa ttagttttga aaacagtggc tataagagaa
    15901 gctcatcata gagtaaaaaa taatcttcat acagtaatat ctataataaa aaaacaaagt
    15961 agattatctc aaaatgatga agtaaagcag tgtttagata acataataaa tagggtattt
    16021 gcaatacttt cgactcatta tttactatct aaagaagttg ataataatat atctatacga
    16081 gaaggtatca atttattagt gtcaaatatt caaggtggct atttatttag taaggatata
    16141 gatatacgta taactggtga agattttaaa attagtggag aaaaagcaac agcaatactt
    16201 ttggttataa atgaaatact acaaaattgt tatgaccatg cattttcgga tagagaacat
    16261 ggtactatac aaatattaat aaataaagat aacgactata gaaatattgc tattatagat
    16321 gatggaattg gatttgatag taataatata aataaaaata gcttagggac atatataata
    16381 gatagttata taacacagat gttaaaagga aatatagaga gaaaaagtag tgagaatggt
    16441 acagaagtat taattactat acctacacaa attaatattt aaataataac tcatctacaa
    16501 agtagtagtt ttaattgagc aaagaggctt aaggttatta atagattaag ttaattgaat
    16561 gtatttataa aaattataag tatatattca atttaacagg tatttattat aactttaagc
    16621 ctctttgctt gtttaagaca tacttaaatt ttaggaggtg agaaaatgag agaagagtta
    16681 ttaagtgttg gtattgacat tggaacaagt actactcagt taattttttc taaattaata
    16741 gtagaaaata ctgaatcaaa tttttcggta cctagaataa gtatagtaga caaagaaatt
    16801 atttataaaa gcaaagttta tttcacccct cttatttcaa ataatgaaat aaatggtcaa
    16861 caaataaaag atatagttga aaatgaatat aaaaaagcaa atatatctaa agaagatata
    16921 catacaggag cagtaataat aactggtgaa acagctagaa aagaaaatgc aaatgatgta
    16981 cttcatactt tgagtggatt tgctggggac ttcgttgttg caacagcagg acctgattta
    17041 gaaagtataa tatcaggaaa aggagctgga gctcatattt attcaaaaga acattcaact
    17101 acaattgtaa atatagacat aggtggggga actagtaaca tagcctcatt taaaagggga
    17161 gaagtaaccg atactgggtg tttggatata ggtggaaggc ttgtaaagat agataaaata
    17221 agtaaaaaaa taacttatat atcacctaaa atagataaaa tcataaaaga taataattta
    17281 aatcttcaag taggcacaat ggctactgta gaaaatacaa agccaatagt ggatataatg
    17341 gttgacttat taatggaaag cataggatta aagaaacaga cacctttgtt tgattttata
    17401 ataaccaata aatcaataag tatagatgaa aatattaaaa atgtttcatt ctctggtgga
    17461 gtagctgatt atatttacta tgatggagaa ataaaagatt attttgaata tggtgacttg
    17521 ggaattttac ttgggcaagc aataaaaaat tgtgaatacc ttaaaaaagt aaatgtagtt
    17581 aaatctatgg aaacaataag agccacagtg gttggagcag gttctcatac tactgaagta
    17641 agtggaagta caataactta cactagcgaa aagtttccac ttaagaactt gccagtttta
    17701 aaattatcaa ttgatgaaga aacaggcact tcaaaacaaa tcagtgaagc tataaaaaat
    17761 aaacttaatt ggtttaaact agaaaatgat ttacagaaag ttgctatagc catgatagga
    17821 aagaaaaatc caaccttttt agatatacaa gagtatgcta aagggtttgt agatggaatg
    17881 caacaaatca tagaaaatga tttacctttt atagtaattg ttgaaaatga tatggcaaaa
    17941 gtgttaggtc aagcaatata tgcattatta aattatgaaa aagaaattgt ttgtatagac
    18001 agtgtcaagg ttgaaaatgg agactatatc gacattggaa aacctatagt tgaaggtaaa
    18061 gttttacctg tggtaataaa aacattagtt tttaattaat agaaggaggt aagtaaatga
    18121 ttttaaatac aaaattattt ggacgtactt atcagtttaa aggcttatat gatgtaatgg
    18181 ctaaagctaa tgaagaaaaa tcaggagata aattagcagg tcttgcagca gaatcagctg
    18241 aagaaagaat agcagcaaaa gttgttttat cacacataaa attggaggat ataagaaata
    18301 atcctgcagt ggcatatgaa gaagatgaag taacaagaat aatacaagat gctgtaaatg
    18361 aaaaagttta tgaagaaata aaaaattgga cagtctctga acttagagaa tggctattgg
    18421 attcaaatac tacaagtctt gatataagaa gaatatcaag aggattaact tctgaaatga
    18481 tagcagcagt tgcaaaatta atgtcaaata tggacttagt ttatgcagct aaaaagataa
    18541 aggttactgc tcactgtaat acaactatag gagaacaagg aacattatca gcaagacttc
    18601 aacctaatca tccaacagac gacccagatg gaatacttgc atcacttctt gaaggtttaa
    18661 cttttggtat aggagatgca gtacttggat taaatccagt tgatgactct gttgagagtg
    18721 ttactaaagt attaaaaaga tttgaagaag taaaacaaaa atatcaaata ccaactcaaa
    18781 catgtgtatt ggcacacgtt actacacaaa tggaagcaat aagaaatggt gctcctgcag
    18841 atttaatatt ccaatctata gcaggttctg aaaaaggaaa tgaagcattt ggatttaatg
    18901 gcgacactat agaagaagct agacagttag ccttaaaaca cggtacagct acaggaccta
    18961 acgttatgta ttttgaaact ggacaaggtt cagaattatc ttcagaagca catcatggaa
    19021 tagaccaagt tactatggaa gctagatgct atggatttgc taaaaagtat gaaccattct
    19081 tagtaaatac agttgttggg tttataggac ctgagtactt atatgatgct agagaagttg
    19141 taagagcagg acttgaagac cactttatgg gtaaactaca tggcttacca atgggtgttg
    19201 atgtttgtta cactaaccat atgaaggctg accaaaatga tatggaaaac ttagccatac
    19261 tattaacaac agcaggatgt acgtatttta tgggaatacc tcatggagat gatgttatgc
    19321 ttaactatca aacaacagga taccatgaaa cagcatcact tagagaaatg tttggactaa
    19381 cagcaataaa agaatttgat gcttggatgg aaaagatggg attctctaaa gatggtaagt
    19441 taacagagtt ggcgggagac gcatctgtat tattaggata ggaggtattt aatatgaatg
    19501 agaaggattt aaaagcattg gtagaacaat tagttggaca aatggttgga gaattagata
    19561 ctaatgttgt gtctgagact gttaaaaagg caactgaagt tgtagtagac aacaatgctt
    19621 gtatagatga tataacagaa gttgatataa gaaagcaatt actagtaaaa aatcctaagg
    19681 atgcagaagc ttatttagat atgaaagcaa aaacacctgc aagattaggt ataggaagag
    19741 ctggtactag atataaaaca gaaacagttt taagatttag agcagaccat gctgctgcac
    19801 aagatgctgt attctcttat gtagatgaag aatttataaa agaaaataat atgtttgcag
    19861 tggaaacttt atgtaaagat aaagatgaat acttaacaag accagatttg ggaagaaaat
    19921 tttctccaga aactataaat aatataaaat ctaaatttgg tacaaatcaa aaggttttaa
    19981 tattagttgg agatgggctt agctcagctg caatagaggc aaatttaaaa gactgtgtgc
    20041 cagctataaa acaggggcta aaaatgtatg gtatagattc aagtgaaatt ttatttgtta
    20101 aacattgtag agttggtgct atggaccact taggtgaaga attaggttgt gaagttatat
    20161 gtatgttggt tggagaaaga ccaggattag ttactgctga atcaatgtca gcatatatag
    20221 cttataagcc atatatagga atggcagaag caaaaagaac agttatatca aatattcaca
    20281 aaggtggaac aactgcagtt gaagcaggtg ctcatatagc tgaacttata aaaactatgt
    20341 tagataaaaa agcttcagga atagacctta aataaagtcg agggagtgta gaatatgaaa
    20401 aatgatgtaa ttcgtcctaa tatactagga attaaaataa tttcaaatat aagtccagaa
    20461 atggcacaaa aactagaatt aaaatcacat cacaaaagct taggtttaat aactgcagac
    20521 tgtgatgatg ttacgtatac agctcttgac gaagcaacta aagcttcaga agttgatgtt
    20581 gtatatgcaa aatcaatgta tgcaggtgct gcaaatgctt caacaaaact tgctggtgaa
    20641 gtattaggaa taatagcagg tccaagccca gcagaagtaa gaagtggtct aaatgcagta
    20701 gttgattttc tagagtatgg agcaactttt ataagtgcaa atgatgatga ttcaatagca
    20761 tactatgctc actgcgtatc aagaacaggt acataccttt cagaggtagc aggaataaga
    20821 gaaggtgaag cattagctta tttagtagca ccaccacttg aagcaatgta tgcactagat
    20881 gcagcaatga aagctgcaga tgttaagatg tgtgaattat ttgcacctcc aactgaaaca
    20941 aactttggtg gagcattact tacaggttct caatcggctt gtaaagcagc atgtgatgca
    21001 tttgcagaag cagtaaaatc agttgcagac aatccaacag gattttaagg atagttagat
    21061 ggatgctata ggcttaatag aagtaatagg atatgttgct gcaatagaag cttctgatgc
    21121 ttgtttaaaa tctgcaaatg ttaatattgt aagagttgat aaggttggag caggtattgt
    21181 tactttaaca atatgtggag atgtcggtgc tgtaaaagct gctttagaag caggtgaaat
    21241 agcagcttcc agagttggta cattaagaac atcacatatt ataccgagaa tccataatga
    21301 agtaacaaat gctttattca aaactaaaga acctaaagtc tgtgaagttt cagaagataa
    21361 agaaaaaaac tttgagatta cattggaaat agctgataat gtatcgaaaa atttatcaga
    21421 aaaagaaaat gtaattttag atgtcaatga aaacagtgga gatactgaaa atttatatag
    21481 tgatatagat aaaacaaaca aaaaaattga aacagttgat gatagtttaa cagaagctaa
    21541 tgaggaaaaa atagaaaaca ctaatgataa agaagtattg gaacaagtta aaatagaaaa
    21601 ttccgatttt gatagagatg acctatcaaa gaacagtgta aaaaaactta aagctatggc
    21661 taagaaatta gattcttcaa taacttataa agacttaaat gttcttaaaa aagaagaatt
    21721 aataaactta gttaaaaagc ttagtagaag ggatagatag ttatgacact tttagataaa
    21781 gatttagtat ctatacaaga agtaagaact cttgtaagaa gatctaaagt ggctcaagag
    21841 aaactttcta aaatgagtca agagcaaata gataaaatag taaaagctgt tgtagatgca
    21901 gcatttaata atgctactaa attagctaaa atggcaaatg aagaaactgg atttggaaaa
    21961 tgggaagata aagtaattaa aaatactttt gcatctatgg gagtttatga aactttaaag
    22021 aacacaaaaa ctattggaat aattggtgaa gataaagaag ccaatacgat agatatagct
    22081 acaccagttg gagttgttgc aggtcttata ccttcaacaa acccaacatc aacagtaata
    22141 tacaaaacta taatatctct aaaagcagga aatactatag ttttctctcc acatccaaat
    22201 gcaaaaaact gcatacttga aacagttaat ataataagtg atgctgctat aaaggctgga
    22261 gctccagagg attcaatatt atgtataact aatccaagta tagttggaac caatgaattg
    22321 atgaaacata aagatacaaa cttaatacta gcaactggag gagaagcaat ggttagagct
    22381 gcttactctt caggaacacc agctattgga gttggaccag gaaatggacc agcatttata
    22441 gataaatcag caaatgttaa attagcaatc aaaagaataa tagattctaa aacttttgat
    22501 aatggtgtta tatgtgcatc agaacaatct atagtaatag aaaaatctat ggaagaaact
    22561 gttttaaaag agcttaaaaa tcaaggtgca tatgttttaa atgaggagga aagtgaaaga
    22621 ttagcaaaat tcatattaag agctaatggt actatgaatc cacaaatagt tggtaaaagt
    22681 gttgaggtaa tagcaaattt agcagactta acaaatgtac ctaaaaatac aaaagttcta
    22741 gttgcaagag aaaataaagt tggtcacaat gtagcctatt caagagaaaa acttacacca
    22801 atactagcgt tctatgttga agaaaatgta gaagctatta tgaacagatg cagagaaata
    22861 ttgttaaatg aaggtgcagg tcatacattc tcaatgcatg taaatgatga agaattggta
    22921 aaaagatttg cccttaatat gccagtatca agaatacttg tgaatactcc ttcagcactt
    22981 ggaggaatag gtgcatcaac aaaccttgta ccagcactaa cacttggttg tggtgcaata
    23041 ggtggaagtt caacttcaaa caatatagga ccacttgatt taataaatgt taaaagagta
    23101 gcttatggaa tcaaagaaat agaagaactt ggaagagata aatctcaagt tgaaaaatca
    23161 atgtttgact caatggataa agaagaatta ataaatcttc ttgttcaaaa actagtcaaa
    23221 gaactagcat aactaaaata aacaatgaaa cttatggcta tcgctataaa tttctaaaaa
    23281 tataaactat aaaaaactat aaaattaaat ttttggagga taagattatg gcaagtgcaa
    23341 acgcattagg aatgatagaa actaaaggat tagtaggagc aatagaagca gcagatgcaa
    23401 tggttaaagc tgcaaatgta caattagtag gaaaagagca agtaggtgga ggattagtta
    23461 ctgtaatggt aagaggagat gttggagctg ttaaagctgc tacagatgca ggagctgccg
    23521 ctgccgagag agttggagaa ctaatatcag ttcatgttat accaagacca cactttgaag
    23581 tagatgctat attaccaaag gtatcagctg agtaatataa gaataattat aaaatttaaa
    23641 ttttgattga aaaagaggct atctcaaaaa tgagtcttac taataaactc aatgtatttt
    23701 gaggtagtct tttttataca tctaatagga gatgtgataa aatgggtgtt ttaacagaaa
    23761 acgaagtaaa aagaaaaatt ggaaaaaaca aaaataatat aactgaattt tatatagaaa
    23821 aaggtcaaat aataactcct tctgcaaaat cttatttagc tgagagaagt attgagctta
    23881 aatatgttga taaactttca gatgtaaaca atgaacctaa agaagttgta aaagaagtag
    23941 ttaaagagaa aaaatataga tatactactg tatttggcgc taaattggat gaaaaaccag
    24001 aacatatgac tcaattaaat ggaaatcttt tagtttttaa agaccataaa agaataatat
    24061 tgagaggaaa aatagattct cttgaatcag aaatactaaa ggtacagata ctttgtcaga
    24121 aactagatat gccaaagctt gttaaggaat tgcaagaagt attagacttt gtaagaaata
    24181 taatgagatg tgaagtttta gatgaaaagc taggggactt taaactttta ggattaactc
    24241 ctcaagaact tagagaacaa tctcactatc ctaaaaaata ctttggtata ggacatgagt
    24301 tcccaagtta tgaaatggga gaagtagtag tagctataaa tagtataaga agtaatacac
    24361 gagaggttga acttgcagcc tatgaagcat ttaagggtga gtatggaaat gttcacagag
    24421 aagatttaat aaaggcactt aatagattat cgtcagtatt ttggataatg atatataagg
    24481 ttagaactaa taagtataag tagttggagg acataatgga gaatctatta aatgaaatag
    24541 tggaagaagt aataactaga gttaaaaaag aagcatttat agaagttgaa gcatcaggaa
    24601 gacacgttca cttaagtaga gaagatgtcg ataaattatt tggagaagga tatactctta
    24661 caaaactaaa agacctatct caaccaggtc aatatgcatg taaggaaaga gttacaataa
    24721 ctggtcctaa aggaagtata aaaaatgtag ttgtacttgg accatgtaga aatgaaactc
    24781 aggtagaaat atctttaaca gatggttcaa ctcttggact aaaagctcct ataaaacaaa
    24841 gtggagactt agaaggtact ttaagtatta aaatatcaac acaatatggt gaagttgaac
    24901 ttgataaggg gttaatggtt gctaaaagac atatacacat gactcataaa gatgctgaaa
    24961 aattcaatgt atgtgataaa gagatagttg aagctaaggt gatgggtcaa agacctttaa
    25021 catttgatga tgtagtaata agagtgagtg acagttttaa aacttatatg catatagact
    25081 atgatgaagc caatgcatgt ggttattcaa aaggaactgt tgctaaaata ataaagaaag
    25141 cgtaaattta acctactata taaattacct ctagggagga tttaatatga attatgataa
    25201 ccttgtagat ttaataacgg aagaaatcta taaaaaaata aatagtaatg aaataaaaat
    25261 atctaataaa cctaaagctg tgattgtatt tgaacaagat aacaataaat ttaacttatt
    25321 aaaagatgaa tttgaaatag tagaatttga taaaagtata agggaatgtg aaatagtgat
    25381 agtttcaaga ctttgtatga gggggctatc gaatatagca cttggaaatt caacaagtga
    25441 tgaagaaaga tttatactaa aaatgattat gaagggcaaa gaagtatatg tcctagatga
    25501 aggaattgaa tacaaaaaat ataaggatac tgcaccaaaa gcattatata agaaatttat
    25561 gtcatttgaa gatgaaatat gtaaatttgg tgtagaaatt ataaaagact taaatgttat
    25621 aactaaaaat aagttaaata taaagaaaga actagaaagt actagaggtt ctaaaaaaga
    25681 agatacaaaa gttttagata aagatgtttt aaatttaacc agtgtagaaa gtgtaaaaat
    25741 agataatgaa tttagtttag atttaaggaa taaaaaactt atatcggaag ctgacttaag
    25801 aaaaccaact ataaatggag ttaaaaacat attagttaat aaaaaaagta taataacacc
    25861 attagctgta gattttatga gaatacacca tctcaaacta aagaaagtgt aaggaggtaa
    25921 ttttatggat ataggaaaag ttgtaggtaa tgtttgggct acaagaaaag atgaaaaatt
    25981 atgtggccaa aaacttttag ttgtaaaaat tttaaaaact aaagacacat acaaggaagg
    26041 cttcatggtt gcagccgata atgcaggagc aggaaatgga gacttagttc ttatatccaa
    26101 aggttcctca gctagagagt cgattgaaaa atctcatgtt ccggtagatg caactatagt
    26161 tggagtagtc gattcgtttg aggtgtttga tgaataaaag tataggagct atagagttta
    26221 aaagcattgc taaaggtata gaagtatcca atgaaatgat aaagaaatct tcagttgatg
    26281 tattgtactt aaaaagtatt tgcccaggta aatttctaat aatagtaggt ggagaaactt
    26341 cttatataaa tgaatgtgta gattatggaa taaaacttgg agaaggatat attgtagata
    26401 acttcgtaat aaatgcaatt tcacaagaaa tattagatgg atttaaaaat aagtatcaaa
    26461 agctagatag tatagtctct ataggcgttg ttgaaagtag taaagtatgc gctggaataa
    26521 aaatgcttga taagacttta aaatctggcg acttagtatt agtaaaactt caattatctt
    26581 ttgctatagg tggcaaatta gtatatatag tagcaggaga tttaagtagt ttagaatatg
    26641 cattaaaaga aagtgaaaat gtagtaagag aaaaagaagt aatatataag actgtaatac
    26701 catcagtaga cagtcaaata ataaaaagtc taataaaata atggggggaa ttatgagtat
    26761 aaacgaaatt ataatctatg taatggtagt cttcatggta cttggagcta tagataaatg
    26821 tataggtaac aaatttggtc ttggagaaca atttgaagaa ggtataatgg caatggggtc
    26881 acttgcagtt gcaatggttg gtgttatatg tttagcacca gttcttgctg atgtattaag
    26941 accaattgta gtaccagtat ttaatatact tggagctgac ccagctatgt ttgctggaag
    27001 tttattagct aatgatatgg gtggtgcacc acttgcactt gaacttgcga aagaccctaa
    27061 tgctgggtta tttggtggat taatagtagg ggctatgatg ggacctacaa tagtatttat
    27121 aataccagtt gcgcttggaa taattgaaaa agaagaccaa aaattcttag caacaggaat
    27181 acttgcaggt ataataacaa tacctattgg agcattcgtt ggtggattag ttgcaggatt
    27241 cgaggtaatg atggttctta gaaacttagt gccaataata atatttgcag tagttatagc
    27301 acttggatta ttaaaattcg aaaatgctat gataaaagga ttcacatact ttggaaaagg
    27361 tgtagtaata gttataactt taggacttgc tgctgccata gttgaagctt taacaggaat
    27421 agttgtaata cctggaatga aaccaatcag tgaaggtata gaaatagttg gagatatagc
    27481 aatagtttta gcaggagcat tcccattagt gtttgttata acaaaggtat ttaataaacc
    27541 attaatggct ttaggaaaag ttcttggaat gaatgatgta tcagcagcag gccttgttgc
    27601 aagtttagct aactgtatac caatgtttgg tatgatgaaa gatatggaca atagaggaaa
    27661 aataataaat gtagcattct ctgtatcagc agcattcgta tttggagacc acttaggatt
    27721 tacggcagga tttaattcta atatgataac accaatgata gttgctaaat tagttggtgg
    27781 tataacagca gtattgttag ctatggttat cgcaaataag acactaaaga aggagaatgt
    27841 ataagatgga tatatcaaat atagataaaa atttaattga aacattagta agacaaataa
    27901 tagaagaaaa aataagtgga actaaagaca cagtagattt tgttagaaat aaagacatta
    27961 gtggaataac ttctataaag ttaccaactg taaaagttag tgaatctgat agattagata
    28021 cagggaatcc aagtgatgta gtttacacaa aagatttatt tacattagag gaaagcccaa
    28081 gattaggttg tggtatgatg gaaatgaaag aaacaacttt tgattggact ctaaactatg
    28141 atgaaataga ttatgtaata gatggaactt tagatataat aatagatgga agaaaggttt
    28201 ctgcttcttc aggtgaatta atatttatac caaaaggaag taagatacaa ttttctgtac
    28261 ctgattatgc tagatttata tatgtaacat atccggcaga ttgggcatct caaaattaat
    28321 aaaaatcaag ttaagttaat aataatcaaa taaataattt aaaatacaaa ctcttaaata
    28381 agcacacata tttaaaaaag ctatgaatta aaattcatag ctttttttat aaagaatgtt
    28441 atattttaga cgtatttttt ctatatttac tgggagacat attatatttt ttattaaata
    28501 atctaataaa atgctctaca tttggataac caatactatt ttactgataa tcttttcctt
    28561 atcactaatc ttacatttta aaatattatt tttactcgta atattatcat aagaatttgt
    28621 ttcttattta aaactaatta gaattataaa gtttattgcc atgttattaa taaaataaat
    28681 ccagttaaat cgagtgctag ttaaagtaat aagaaacttt tacaatggtt tcctttatcg
    28741 attgaaataa aagttaatat ggtataatat ctaaagagag aatcttatca attagaatat
    28801 gcatagaaac aagtggctta aaaggagacg ttatatggaa gagatagtaa agaaggaaca
    28861 agcaagtatg aatgatattt ttttgtatct taaagctaaa tcaaactcat tatataaatt
    28921 tgtaatatta tataatgagt ttatcaataa acctaaagat tatggaactg gggaacttat
    28981 aaacatggtt gaagtacata tgctaacagc gattgaagaa aatccaggaa taagtccaac
    29041 tcagttagct aaaatatgga acagaactaa aggtgcaata tctcaaacta tatctaaatt
    29101 ggagaaaaag ggttatataa ctaaagaaaa gaaagaagga aatgcaaaaa ctatattact
    29161 atatccaacc acatcaggag tagagttaag taaggcacat aaactatatg acaccataga
    29221 tgttacaaga acatttgaat acttaatcaa ggagtgtaca ttagaggaga tagatagttt
    29281 ttataaagta attgatttgt ttattaattt attagaagaa tctccgcagg aaaataatga
    29341 ttctgaaaaa gtgtagctga gattgaagtt gaataaagag aaatagatat tatagaagta
    29401 gtacttaagt acacattata taaaaatata ataggtatag gattaaaaag tttagtggtt
    29461 taaagtttaa ccactaaact tttttaatgt ctataatcta ctattattta atttactaaa
    29521 aataaaaaaa ctcattatat taagataaaa atagaaaaat aactagaaaa atactataat
    29581 atatcagctt aatacagaaa aaagttaaaa aatttgagaa aaataaaaat gaaaacgttt
    29641 tatattgaca tagagaaaaa gtagtgatac catttaaata tagtttagta gctaaactat
    29701 atttaaatta taataactat tttaggggga tgttatgaat ttaaaacaat taagtgaaaa
    29761 atacgaagaa tatgtaattc aaatgagaag agaatttcat aagtatccag aattatcttt
    29821 taaagaaata agaacaaccc aaagaataag ggaagaatta caaaaaatag gggtcccttt
    29881 tgtatctatt gaaccaaata tcgtaatagc tactatacaa ggcaaaaatg aaggtaagac
    29941 aattgcaatt agaggagata tagatgcgct tccaatgcaa gaagattcag atgttgaata
    30001 caaatcagaa tatgatggaa taatgcattc ttgtggacat gatgctcatg gagctatgtt
    30061 actaggactt gcacatatgc taaatgatat aagaaaagat ttaaaaggta gagtattatt
    30121 agtttttcaa gcagctgaag aagttggtgg tggacacaag gagataatac agtactttaa
    30181 ggaaaatgga ggaccagata gattaatagc tactcatgtt tggtccgata ttgaagctgg
    30241 taaaatttct gttgaaccag gacctcgtat ggctggtggg tctggttgga gaatagacat
    30301 aacaggtaga ggaggtcatg gttctagacc agatcaaagc atagatccta taaagccagc
    30361 ttgtgatata gttttaaaag tatcttctat accagttaat catataagtg tactagaaca
    30421 atgtgttgta catgcatgtg caatacatgg aggaactaca ataggaaatg ttattccaaa
    30481 caatgcagag gtaataggtg gatatagata ttttactgaa gaatcaggca aaaaagtgtt
    30541 tgacattata aagcaaatga gtaatggtgt tggacaagtt tatggagtgg atgtgaaggt
    30601 tactcatact ggaggcatag gacctgttat caatgatgaa gaagcagtta agatggctaa
    30661 agaaatagtt tctaatatag atggattgga gttagattct tatgagccaa tatgtgcatc
    30721 agattgttat ggtgaaatat taaaagagta ttctggcttt tattgttatc taggggtagg
    30781 taacaaagaa aaaggaataa tatatgcaca acaccatcct aagtacaata tagatgaaga
    30841 tacattaaag ttaggttgcg aatttatggc taaatatgca gttgaatttt taaataaaaa
    30901 taataactaa aataaatcga attttataaa tgggggggat gaaaatgcaa ggtgcattaa
    30961 aggaaaataa gaaaaaaagt ggtggaggat ttttaggatg gattgaaaaa gttgggaata
    31021 aaatgccaca tcctctagca ttatttacat atattacatt gatagtagta gcattatctg
    31081 caatagcaag ctttttaggt ttttcagcaa caagtcctgc tacaggaaag ttaataaaag
    31141 ttatgaactt aataagtgca aaaggtttag tactatttat gcaagagttt gttaagaact
    31201 tccaaaactt ccctgtgctt ggagtagttt tagttatggc agttgccaca ggagtttgtg
    31261 aaaagacagg atttttttca actgccataa aaatgagttt atcaaaagtt aaggggaatg
    31321 cggttgtatt tataatagct tttataggag tatttgctaa tcaagctggt gatgctgctt
    31381 ttgtattagt accaactctt gcaggagcaa tattttatgg attaaataga aatccattag
    31441 ctggtatatt ctgtggattt gcatcagttg gtggtggatt tgcaacagct gtaattcctg
    31501 ggggatggga cgttacgcta acgccaataa ctgtatcagc tgctcaaact attcaacctg
    31561 catttgatat gacattatta gctagttatt atgcactatt tgtatctgcg tttatagtag
    31621 ctacagtatc aacaatagta acggttaaat ttatagaacc taaattaggt aagtatactg
    31681 gtaaaccaga aggtatagat gataatcaag gttttgaggt cacaaaagaa caagcaaaag
    31741 cagcaaagtt agcaggtttt acagttttag cttatgtagc gttattaatt gctttgtgta
    31801 taccagctaa tagtttttta agaagtccag aaggttcttt aatatttgga gcaccactta
    31861 tgtcttgctt acagtttttt atagtaatac tattcttctt gccagggatt gtgtatggaa
    31921 tgagggttgg taaaatcaag actgtaaaag acttaaatga tatattatgt caatcaatgg
    31981 cagcaatggc accgtttatc gtacttgcaa tagttatagg tcaattctta acgctattct
    32041 cagtgtctaa tttagcacag gtactagcta taaaaggtgg agcattactc aactcattgc
    32101 cactaccacc acaagctata atactgctat tcttagtact agtagcgttt ataaatatat
    32161 ttgttataag cggaagtaca aaatggatga tatttggacc agtatttgtt ccaatgttaa
    32221 tgcaattaaa catacatcca gcatttactc agtttgtata tagattagga gattctgtaa
    32281 ccaatcattt atcaccatta aatgcattct ttataatact tttagcaaca gtccaaaaat
    32341 acgataaaaa tgcaggtatg ggaactatat tctcagctat gataccttac actataggtt
    32401 actttgtagt acttgcagtt ttagtagtag tttggatgac aataggtata ccagtaggaa
    32461 taggtggaca agtttggtta taaaacatat ttaataattt tgggaggaaa gaaaatggaa
    32521 acaactgaaa taagatacaa ttttgatgaa gtaatagata gaagtggaac aaactgtctt
    32581 aaatatgatc ttaagagtca atttatgcca gaatctccag aagatgcact accactatgg
    32641 gtagcagata tggattttgc atgtgcccct gagataatta atgcaatgca tgaaagaata
    32701 gacagaaaaa tatttggata tagctctcaa caaactgatg attattatga agctgtatgt
    32761 ggatggttta agagaagatt tgattggaaa atagaaaaag agaatatatt ctttagccca
    32821 ggggttgtgc ctgcgttagg atttttcaca caaatattaa cacaaaaggg tgatggtatc
    32881 ataatagaaa agcctgtata ctatcctttt gaagataaaa taactaataa tgaaagaaaa
    32941 gtagttaata gtcctttaaa gtttgaagat ggtcattatt ctatggatta tgatggattt
    33001 gaagaattag ctaaagatga aaataataag gtgtttatat tatgtagtcc tcataatcca
    33061 gttggtagag tatggaaaaa agaagagctt agaaaaatag tagacatatg taagaaatat
    33121 gatttgtgga taatatctga tgaaatacat tgtgatatta ttcgtaaagg tgaaaagcat
    33181 ataccattag aagtagcatg tcctgattat aaagatagaa taataacttg tacagctcca
    33241 agtaagagtt ttaacttagc tggtatgcaa ttatcaaata tagtaatcaa taataaggaa
    33301 ttacaaaaaa aatggatgct tgaaacagat tctaaattag ctatattcct acctaatcca
    33361 ttcgcaatag ttgctactaa agttgcttat aatgattgtg aagattggat gaatcaagta
    33421 aatgagtata tagatggtaa tattaaatat gtagaagatt ttgttaatga gtatatgcct
    33481 aaagtaaaga taataaaatc tcaaggaact tacttagttt ggttagactt tagagggtat
    33541 ggattagatg caaaacagct tgaatatgta atgcttaaca aggcgaaggt attgctagat
    33601 gaaggataca tattcggaga agaaggagca ggatttgaaa gaataaatgt tgcttcacct
    33661 agaacaatta tagaagagtg tatgaataga ataaaatgtg catttgaagg attataatag
    33721 taatatctaa ataatatatt tgtgtatttt aaaaaaagac tatgctaaaa aaatatttca
    33781 aagcatagtc ttttaaagtt ttatgatgag tttaaaaaat ttcaaaacgt tactattcac
    33841 ttatatgttt atattagaaa taattccaca atatactcat tttctaaagt tgcactttta
    33901 tacaccttgc ttcaccaata tcatgtgtaa ccaaaatagt agacttattc tcattcttaa
    33961 taatactata aacatcatca caaactaata atctagtctg ataatcaaga gctgaaaaag
    34021 gttcatctaa aagtagaata tctggattta cagaaagtgt tctaataagc gcaactcttt
    34081 gtctcatacc tccagataat tcttttggat acatacttct aaaatcccat aatccataag
    34141 tctttaaaag gctttcaact ctatctaaag attttttatc ttttttgcct tgaatttcca
    34201 atccaatagt tatattatcc attatatttc tccattcaag caaattatct ttttgaaaca
    34261 tatatccaat atttccagta atctttatat caccactagt cggttttaaa agattagtaa
    34321 gaatatttaa tattgtagat ttaccacttc cagaaggacc aagcaaagtt aatatttctc
    34381 cttctttcaa gttaaaattg acatccttta atacttcaag ctcgccttcc ttagtgtaga
    34441 agttcatatt tatattttta acttctacta tgttgtcatt cattatgcca ccccctataa
    34501 cttttagctc gtatatatta tgttattaac aatatgacct atttgtacca tttttcataa
    34561 atataatata aatattttgt ttatttacgt attttcttag taacttgtac gtataaatta
    34621 tagataaata aattataagg taggggataa atttgagtaa cataataacg aatttaatag
    34681 agtcaatatt tactaatcca ataactatat tgtttttact tgcaataact gtgtattctg
    34741 tttttaaaat aatagaaaat aacagagtat attcatttgt aagttcaaaa ttagacgaaa
    34801 tcaatagaga ctataagaca agtgagtttt ataaaaaggt aagggatgac tactatacat
    34861 atagtaaaga aaatccttat gcagacgtaa atataacttc atttatagag gaagttgtat
    34921 ctgatttaaa gcataataat ttgccattgt tagaaaaaat tagaagtata aaaaattcat
    34981 cttcaataag tatattactt ggggttttag gtacgtttgt tggtctttct acaatgcttt
    35041 tatgtgttga tactaaagac attataaatt cacttccttc aactattagt agtatgcaaa
    35101 ctgcatttac aactagtata tttggagttg tattttctct aattataggt tactttacta
    35161 agataaaaga ttgtgaacat gttttgatac aaataatgtt aaaatctgaa aatttactaa
    35221 catctgaaat aactcatttt aagtcagaaa gaatggattt aaaagtagaa gaggtaaaaa
    35281 atacaataaa acaaataagt aagtctatag aagctattga aaaatttgat aaaatatcta
    35341 aagatttaaa tgactttaat gatgaattta tcagtggtat agaagcttta aaaagccttt
    35401 tagaaggttc tcaaagttct attaagacat ttgaccagag cgtaagaaaa ttagataaac
    35461 agtttaatat tttaaatata aaatttgtaa agctttttga taaatatgat aatcaagata
    35521 atataaataa agagatttta tttgatataa aagaatcttc aaaaaatata tacaatgcca
    35581 ctgaaagcca attcaaaatt agagattata taaaaaatat aaatgcagga tttgctctat
    35641 atgaaagaag tgctcaagat ttattaacta aattaatgac ccatgaaaat aaaatatctc
    35701 aaaatcaaaa aatcctttta gatgaaaagt ttaccttaga tgactcaata aaaaatctct
    35761 caagtattat tgaaaacttt tctaatgacc tacaagttaa actggatatg atgtttgaaa
    35821 attctttaga tattcaagat aagttagatg ttatgtttaa taactctttt atgaatgatg
    35881 aagttcctct tgatagtgaa gaattattta aggatgatgt taataatgta tttaatcctt
    35941 ttagtgaaga aatttatgag atagaggata aagaaataaa ggtcataggt gaggatgagt
    36001 taaatgaata ataaatatag aagaactgtt aacagagaat ttgaaaagag tagcttttgg
    36061 ccaacattta cagatttatt atctactgta ttgatggttg tgattcttat cttatttagc
    36121 tcagaaagta tttcaggttc tgttgaacaa gatttagcta agaatgtcaa tgcttctgtt
    36181 gaagaaactt ttaaaaaaag tggaatacct gtgaaggtag ataaatctaa tgggcaagta
    36241 acttttggtg aaaaaaccat gtttgatgta gatagtgatg ttttaaaacc agaggctaaa
    36301 gaaatgctaa aaatgtttgt tccaaagtac atagagacta tttataaaga ttatggtgac
    36361 tatatatcca aaatagttat agaaggtcat acagatgatg tgggaagtta tatatacaac
    36421 ttagatttat cgcaaagaag agcatatagc gttgctaaat ttatagtggg agatgaaata
    36481 ggtgactata aatacaaaga taaagtgact aagcatatta ttgcaatagg aaggtcaaaa
    36541 gctgaactca tcaaaaatgg tgacaatagc gtgaatagag atgcatctag aagagtagaa
    36601 ctaaaatacg aaataaatat taatcaaaat aaataattaa atttttataa aaaaaaagaa
    36661 ggcagttggt gtgagcttta agccttcttt aaggaaaaag tttatttatt tttgttgata
    36721 cttaacatat tatagttttt agaagcagtt tgcaatatat taaacgtaaa tggtcgttta
    36781 aaatgcataa ctggatacta ctgatgtaaa atatttcatt ttatataata taaaaattat
    36841 acagtgtcaa ttttctatat tattgtacta taatctatta tttaaaatat tatgtaaaaa
    36901 aatatcataa aaacgcatat catgttataa ttactagagt attatgaata tggaggcgta
    36961 gtttgtgata gaaataagta taaaaaataa aaaaaattct aaaattaaaa ctttgatact
    37021 tttattgata gtatctatat cgatttattt aactggttgt actacatcag ataatggaaa
    37081 taatcaatca aataataatg attctttgtc tagtagtata tcaagtgata agaatgtaaa
    37141 aattcatttt attgacactg gaaatagtga tgcaatattg attcaagatg gtaaaacttt
    37201 tacattgata gatgggggag ataatgatga tgagggatta atggtagatt acctaaacaa
    37261 tcaaggtgta aaagatatta aatatcttat agctacccat tctcatgcag accatttagg
    37321 tggacttgat tctgttgtca aaaactttaa tatagaaaat gtttttgtat cgaatggttc
    37381 agcagaaaca aagagttata gggactttat aaatgcttta gcaaataagg gtttatcccc
    37441 aagtgtaccg cttgaaaata ataagttcta tttagaagat tcttattttg aagttctaaa
    37501 tacaaatggg ggagatacta ctaatgaaca atctctagtc cttgtatata ctaatgggaa
    37561 tgataaagtt ttatttacag gtgatgctga agaaggtacc gaaaaagaaa tactacctaa
    37621 gcttgagaaa gtagaccttt taaaagtagc tcatcatggt tctcgtagta gttcatctca
    37681 agagttttta gataaagtaa atcctgaata tgcggttctt ttagtaggca agggtaattc
    37741 atatggtcat ccacatcagg agacaatgaa taaattaaaa aaaatgggag taaaagtgca
    37801 tagaagtgat gagtgttcag atataatatt tgaatcaaca ggtgatggag tatttacaag
    37861 ttgtaaagat ggaagttata ataaaggcgt tagagaagat ggaaactctg gaaaatcaaa
    37921 cagtaataca agtaaaaatg attcttttaa aaataaacaa aatacatcca attcagaaga
    37981 aatagtgtat tttactccaa aaggtaagag ctatcactct acaaaaaatt gtagtgggct
    38041 ttcaagaagt aaaaaaatat tatcaggcac aattgcagaa agtaagaaaa atgacccatg
    38101 tgatatatgt tatggaaaat aacaaaaaaa caaccaattt atctataaag cttctgaact
    38161 ttaaatcatt taaaaaataa aataaatgca taaaaaaata agctataatc aatagataac
    38221 acattttatc tatacgaata gcttattttt ttaagtattt tttaatagct atttaataac
    38281 tctaaataca ccagttacaa tacctacaat ttcaacttct gagtcattga gcataattgg
    38341 ttccataaaa tcattttctg gctgaagtct tattaaattt ccttctttaa aaaacctctt
    38401 aaccgtagca gattcaccat ttataagtgc aactactatc tgagaattta aggctgtatt
    38461 ttttttgtct actacaacgt aatcttcatc taaaattcct gcattaatca tactttcgcc
    38521 ttttactctt aatacaaaat tgttactgcc ctttactaaa ctggcaggga atggaatgta
    38581 ttcctctatg ttttgttgag ctaaaatggg ttctccagct gttatttgac ctactaaagg
    38641 aagctctatt atttcttgat tagctccaga aacatcattt actttactac gttctaaaac
    38701 ttcaatagct cttggtttag tagggtcctt tcttatgtat cccagttttt caagtttatt
    38761 tagatgagag tgcacagttg aagtagatct taaccctaca gcagtacata tttctcttac
    38821 agcaggtgga taacctttta atatgatttg agactttata aattccaata tcaagacttg
    38881 cttttcagtt agatctaaat acataacatc actcatttcc aaacatattt tgtttttatt
    38941 tccatacact ttatattttt aattataaca taatccattt atatctgcaa acatttgttc
    39001 ctgattttta atcgtcctca atccattcaa aattcttgac tatatcacgt tttacatttt
    39061 tcttatgagc tgcataaagc tgagtagttg tgacattatc atggtcaagt aagttttgaa
    39121 catcatatat agaaaatcct gtttggatta gagaagttgc agccgttgct cttaatttat
    39181 ggggactata tccattgtct ctagtagtat caagtggaat agatgtatat ttttttacca
    39241 gtgttcttat agcctttgct gtaattctct tattttgaag agacaaaaat aaagcatctt
    39301 tttcttcatc atttaatagt tcatctcttg ttctttcgtt ttgcaaataa tcttttatta
    39361 catgttcaca agtatgattt ataggcatta aaacttcttt cccacgtttt ctatatattt
    39421 taaattcacc tcttgaaaaa ttaaaagagg agatattgag ttctctaagc tcatttaatc
    39481 taagcccgta tgtaacaaat aatgctagta tagctttgtc acgtaattta gtttttctcc
    39541 aatatacctt ttctttctct gtaagccctt cacctgtttc tactgattca agcatcttag
    39601 caacttcatc tatctcaagg cgcttaatag catctggctg aggtttagga agttttattg
    39661 gattgaatcc atcagttata ttattatcta tctgagagtt tctgtataag aatttaaata
    39721 gagtagagat ggatgatttt tttcttgcta aagctctatt gttattttca aaaatcaatg
    39781 tgtttttttc tgtatttttg taatatctac tgcaataatc acctaagaat aaatttatat
    39841 ctcttgattt tattgtatta aaatcatctt ctgtaatgtc ttttacacaa tctgcatttg
    39901 ttaattcttt ggtttcaatc aaatatgaac aaaaaaaatt aatatcttct aaataagcaa
    39961 gtcgtgtaga aactgcaaca gaacctttta aatatataaa ataatccttc ataaatacag
    40021 gaagtttatt ttccaaaagt agacattttt ctatgagttc attttctttg ataacaatat
    40081 tatcaggttt atctgattta ttatgtactt ttataatttt ct
//
LOCUS       DAYAPA010000024        38206 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.024, whole genome shotgun sequence.
ACCESSION   DAYAPA010000024 DAYAPA010000000
VERSION     DAYAPA010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 38206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38206
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.024"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(35..574)
                     /gene="scpB"
                     /locus_tag="ACGI59_001130"
     CDS             complement(35..574)
                     /gene="scpB"
                     /locus_tag="ACGI59_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HFG9738933.1"
                     /translation="MKREDIKYIIESVMFAYGEPISIKELNYIINKELSSKEIEIMLN
                     LLIEEYREQNRGIQIIKLENKYQMCTNKDYAEYIKKIIEPKKKKSLSQATLETLTIIA
                     YKQPITKVEIEDIRGVKCDKVLQTLFENELIREAGRLNKIGKPIIYKTTDEFLKLLNI
                     ESLEELPPIENYQEVATNE"
     gene            complement(576..1295)
                     /locus_tag="ACGI59_001131"
     CDS             complement(576..1295)
                     /locus_tag="ACGI59_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HFG9738934.1"
                     /translation="MKYNIQLQVYEGPLDLLYDLITKHKIDIKDISIIDITKQYLNYL
                     KMLDKMDLEITSEFITMASKLLEIKSKYLLYKQKDEEEDPRIELMEKLEEYRKFKVAS
                     QDIKENITYVNERFYRNKEEIIIDDNVDLEDISIEAIKNILPYIFKVKTSQIENANDE
                     KLDKIVRKKIISVEEKILYIRDIIKEKIEVTFTNIIKSYENDEIIATFLSILELIKEK
                     EIVVVQDIFFDDILIRKSSEC"
     gene            complement(1325..1921)
                     /locus_tag="ACGI59_001132"
     CDS             complement(1325..1921)
                     /locus_tag="ACGI59_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HFG9738935.1"
                     /translation="MLSMNIGEIVASLVGIAMAISIHEFGHAYSAHLLGDDTAKAYGR
                     MTLNPVRHVDPIGLLAMFILKIGWAKPVPVNPNNFKNYKIGNLIVSLAGVFCNVLTAI
                     LCVIINKYVHMYAINTIAEYVFLYSIGFAAFNLLPIPPLDGWGVISTFVPYKWNEYLY
                     KFESMSYIILLIALFTGMYRIILTPIYAVLLKIVYLFV"
     gene            2219..3055
                     /locus_tag="ACGI59_001133"
     CDS             2219..3055
                     /locus_tag="ACGI59_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HFG9738936.1"
                     /translation="MRQEERTVCFDRELKIEAYRFKGIMQKFPNHFHEHYVIGFIEKG
                     QRYLSCKNKEYTTSTGDLLLFNPFDSHTCEQIDDKVLDYRCINIKPEIMKKTVFEITG
                     KNYLPKFNQPVIFRSELVPLLQELHYIIMEEELDFKKEELFFFLIEQLIEEHTEPNLQ
                     SNLENTNIEIQAVCDYLENNYAEHIVLDELSTIAGMNKYSLLRNFTKLKGITPYRYLE
                     NIRVNKAKKLLEKGVEPIDAAIQTGFVDQSHFTNFFKNFIGLTPKQYQNIFINDDSNN
                     FK"
     gene            3076..3498
                     /locus_tag="ACGI59_001134"
     CDS             3076..3498
                     /locus_tag="ACGI59_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2000 domain-containing protein"
                     /protein_id="HFG9738937.1"
                     /translation="METKNSKCVMVIDENLPMGIISNTAAIMGITLGKHAPETVGPDV
                     IDKTGNSHLGIIDIPVPILKGNKEIIKDLRKKLYTLEFNDLTVVDFSDVAQSCNLYEE
                     FTQKIASVPEDELQYFGIAIYGNKKKVNKLTGSMPLLR"
     gene            complement(3709..4872)
                     /locus_tag="ACGI59_001135"
     CDS             complement(3709..4872)
                     /locus_tag="ACGI59_001135"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFG9738938.1"
                     /translation="MKKLASTALAILIALTPLSFSFANNKENADANQLNISSKSAILM
                     DVGSGQILYEKDAHKKLPPASVTKVMTMLLIVEALDSGKIKLDDEVQVSETASSMGGS
                     QIFLEPGETQKVDTLLKGIAVASANDACVAMAEHLAGSVEGFVDRMNAKAKELNMNDT
                     HFANTNGLPVANHYTSAHDIALMSRELLKHEMISKYLTTWMDKVVVGKKQVTVGLANT
                     NKLIKHYQGATGVKTGFTQEAKYCLSASAKRGNTHLVAATLGAETSPERFNDASSLLN
                     YGFANYESVKLCSKGDNIATLTLDKADENKVKLVAKEDLNALIKKGSSKEFEKKIEIV
                     KNPKMPIKKGTVLGKIKICKDKKVIGEVELINTKDINKASYLQMLQRIIDNMI"
     gene            complement(4926..5126)
                     /locus_tag="ACGI59_001136"
     CDS             complement(4926..5126)
                     /locus_tag="ACGI59_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD1290 family small acid-soluble spore protein"
                     /protein_id="HFG9738939.1"
                     /translation="MDDVSRQNAIKALKQTKMEIAGEYGMNYEDAFEIIENASNKGVL
                     EGYFKKLEKKKNLGQGISRHLE"
     gene            complement(5332..7008)
                     /locus_tag="ACGI59_001137"
     CDS             complement(5332..7008)
                     /locus_tag="ACGI59_001137"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFG9738940.1"
                     /translation="MQLFKKNTNKIKVMALGGLNEVGKNMTVVEYKDEIIVIDAGLSF
                     PEDEMLGVDIVIPDITYLVKNRDKIKGIFITHGHEDHIGALPYILKKINVPVYGARLS
                     IGLIQVKLKEHKMNNVKLNVIGPRQVIKLDNMEVEFLKNNHSIPDAYSIAIHTDQGII
                     YHTGDFKIDLTPIDGDVMDMHRICELSKKGVLLMLADSTNAEKPGFTMSEKTVGVGLD
                     ELFAKGNGRRIIVATFASNIHRLQQIINTAEKFNRKVAISGRSMVNVVGVAKELGYLD
                     ISDDMLIDLNDICKYEDSELVIITTGSQGEPMSALARMAFSEHKKVEIKSGDLVIISA
                     HPIPGNEKLISRVINFLFEKGAEVVYSDIADIHVSGHACQEELKLIHALVRPKFFMPA
                     HGEYRMLKRHAEIAEQLGMDKENIFVMQTGDVLELDKNSAKVANRIQTGNILVDGLGV
                     GDVGNIVLRDRKHLSEDGLMIVVVTISKDEGKVLAGPDIISRGFVYVRESEDLMDGAK
                     DIIKNVLNECEEKNIKEWAYLKNNIKENLKEYLYQKTKRNPMILPIIMEV"
     gene            7225..7962
                     /locus_tag="ACGI59_001138"
     CDS             7225..7962
                     /locus_tag="ACGI59_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFG9738941.1"
                     /translation="MRGKTHCAIGILTAIQTSLIFKIPISLVDILVSATFAVLPDLDK
                     SNSMVSNFILKNNVSKYIYRIFIYAVNIIIFFISININDNFYLSAIITFVAIIIIEAK
                     LTHTFLRKVFLSLIFILLAICLYIIEVEIYFTIFCLMLSIFPWLKHRSFSHSIFATII
                     VYFLLKQIELITNINNLSFYGTIGYASHMFLGDLFTKQGIPIFYPLSEKKISLGFLTV
                     GGPFSNFIEKSFIFVLIGLIIFSILKL"
     gene            complement(8079..8537)
                     /locus_tag="ACGI59_001139"
     CDS             complement(8079..8537)
                     /locus_tag="ACGI59_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFG9738942.1"
                     /translation="MANTMDLLKDKLKETGFKITPQRRAIVEILLKHDHSHLSSEEIY
                     DLVRVDCPEIGLATVYRTMQLLDEIGLISKLNLDDGCIRYEISLHKEDCHNHHHLICK
                     NCGKIMEAKEDLLDNIEKEIQSLYKFKILDHDVKFYGLCDECNGVSDSEE"
     gene            complement(8819..9106)
                     /locus_tag="ACGI59_001140"
     CDS             complement(8819..9106)
                     /locus_tag="ACGI59_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HFG9738943.1"
                     /translation="MEENIINLIDENGVESQFEIILTLEAEGKEYAILMPLDDEEAEE
                     ALIFRIDEDEEGEILIPLESDEEYETVVAVYTAIMEEEGLNYDEDESNGLN"
     gene            complement(9184..9609)
                     /gene="ruvX"
                     /locus_tag="ACGI59_001141"
     CDS             complement(9184..9609)
                     /gene="ruvX"
                     /locus_tag="ACGI59_001141"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFG9738944.1"
                     /translation="MLDGRIMGLDVGDKTIGVAVSDLMGLTAQGVKTIKRVGKKKDIE
                     ELKAIIKEKQVNKIVSGLPKNMNGTLGPQGEKVIKFCELVKAETGIDVEFWDERLSTV
                     AAERSLLEADVSRQKRKKVIDMLAAVIILQGYLDFKINS"
     gene            complement(9815..11986)
                     /locus_tag="ACGI59_001142"
     CDS             complement(9815..11986)
                     /locus_tag="ACGI59_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9738945.1"
                     /translation="MRKKIMSLFAIVIMIFVSTASYGFAEEKGKVIFIDMNRTNLGSM
                     MNIPILKNEIEKRGYVALMNIRGDQGTDDRRSYASMGAGTRASVTTEDYINFQKVDQD
                     TAKAFEVATGQKAKEINDLGINRSINENLEYGEYGANLGSLGKTLSENGLKTSVIGNA
                     DIIENNQLVKNRNLCLVAMDQYGRIDSGNIDNINKKDSKMPFGISTDYDKLLDETKKS
                     YKNSDAIFVELGDTYRLDMYRLNLNDNTYDIMKKNIVKNINKYLEQVFSMVGENDTVY
                     IASAFPSDLDYKNKRRLSPIIKFSGEGKGLLESATTRKAGIVANLDVGVDILGTFGLE
                     NKEMVGRSYTLVNKEDNISYLKSLYERIVSISSIRSTIINGFVGVISASWVIAMFAIF
                     FRNYMPHKERVFKVLKEFIKLGIIMPLSFMLAPLMNFKTPISISLGIFATTLILYLLG
                     RKLFKDNDIKHMGFFATLTILVIVIDSVFGTYLMQNNIMSYDAVIGARYYGVGNEYEG
                     VTIASAVFGLAVLLHYKKVSKLLVVIFSLIILITSAYPSMGANVGGAISECVAYLLFI
                     MLIYDIKIDFKKAVLLAVSAVVVVFAFAALDILSGSESHLGMFVQRIFLNGPGEILQT
                     FGRKIQMNMQLAQTSVWVNILLVGIGVIAVLIFKPSRHFRKIMNNFPILFKGFIASMV
                     GCIITLLVNDSGIVAASTASIYILIPLIIISINMIIFEKNN"
     gene            complement(11996..12253)
                     /locus_tag="ACGI59_001143"
     CDS             complement(11996..12253)
                     /locus_tag="ACGI59_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HFG9738946.1"
                     /translation="MEKDLDYTMKFEGIPEDRMSVGDTIDFVYKALVEKGYNPINQII
                     GYLLSGDSSYITSHKNARAIIKKFERDEILEEVITHYLNRK"
     gene            complement(12278..14917)
                     /gene="alaS"
                     /locus_tag="ACGI59_001144"
     CDS             complement(12278..14917)
                     /gene="alaS"
                     /locus_tag="ACGI59_001144"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFG9738947.1"
                     /translation="MEKMGLNEIRSKFLEFFESKGHYVANSYSLVPNNDKSLLLINSG
                     MAPLKNYFSGVEVPPSVRMCTSQKCIRTGDIENVGITARHATFFEMMGNFSFGDYFKR
                     ESIKWGWEFVTEWLNIPEDKIWVTVYEEDDDSYDIWAKEMNFPEERMVRLGKDDNFWE
                     IGTGPCGPCSEIYFDRGEEYGCDNPDCKPGCDCDRYLEFWNHVFTQFDRDEEGNYSLL
                     ENKNIDTGMGLERMGCIMQGVDTIFEVDTIKSILEAVEKLTGVKYGENPKNDISIRII
                     TDHIRAVTFLVSDGVLPSNEGRGYVLRRLLRRAARHGKLLGVKELFLQKLIDEVIKVN
                     DKAYPVLVEKESYIKKVVGIEEEKFNETIDQGTEILNSYIEVLKNEGKTVLSGQEAFK
                     LYDTYGFPIDLTKEILEEEHLSVDEEAFNEEMEKQKERARNARGNMDGESWKEDPLSK
                     LESTVDSTFNGYSEIYGEGTIEAIVKDDELVQSAEEGDKVSIVLDNTTFYPEGGGQVG
                     DCGLITNENLVLEVLNTKKGANNSIKHIGIIKSGRISNGDKVKTLVDRETRMSAARNH
                     SATHLLHKALREVLGEHVNQAGSLVTPERLRFDITHFEAISNEELKVIEEKVNNVILS
                     SLDIKCDIMNIKEAKEKGATALFGEKYGDEVRVVSMGDYSTELCGGTHLTNTSQVGMF
                     KILSEGGVAAGVRRIEAITGKAVYEYLKERDGIISEVCVNLKSKEDNLIQRISSLLEE
                     NKNLSKELHDMKAKMSLQSVDSIFDSKVEVNGVNLITNKFEGMDMDTLRETADNLRDK
                     LGSGVVVLANVVDDKVNFVVTATKDVLDKGIHSGNIVREVAKIAGGKGGGRPNMAQAG
                     ASDVSKVDQALSYASEVIKTQVK"
     misc_binding    complement(14985..15223)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(15366..16445)
                     /gene="mnmA"
                     /locus_tag="ACGI59_001145"
     CDS             complement(15366..16445)
                     /gene="mnmA"
                     /locus_tag="ACGI59_001145"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFG9738948.1"
                     /translation="MNKKVMIGMSGGVDSSVAAYLLKQQGYDVIGVTMKLWQDDDVVE
                     IEGGCCSLSAVEDARRVANKIGIPFYVLNFREVFKEKVIDYFIDEYLEGKTPNPCIAC
                     NKHIKFDDFYKKARQIGCDYVATGHYAKIEKDESTGRYLLKKSVTDKKDQTYALYNLT
                     QEQLEHTLLPIGDYEKDRVREIAKEMGMAVHNKPDSQEICFVKDNDYANYVKKHSKKR
                     IEEGFFVDTKGNILGKHKGILYYTIGQRKGLGITFGKPMFVIDINPINNTIVLGDNED
                     LFKKELIAKDVNFISIDTLEEPLRVQAKIRYSAKPSPATIHRVGEDTIKIVFDEAQRA
                     ITKGQSVVMYDGDIVVGGGIIEKSL"
     gene            complement(16734..17174)
                     /gene="nifU"
                     /locus_tag="ACGI59_001146"
     CDS             complement(16734..17174)
                     /gene="nifU"
                     /locus_tag="ACGI59_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold protein NifU"
                     /protein_id="HFG9738949.1"
                     /translation="MQYSDKVMEHFMNPRNMGEIDNASGVGEVGNPTCGDIMKIFLDI
                     DGDVIKDVKFKTFGCGSAIASSSMATEMIKGKTIKDALELTNKAVAEALDGLPPVKMH
                     CSVLAEQAVKAALIDYAQKNNIHIPELDGYVIDDAHDHDVEEEE"
     gene            complement(17228..18421)
                     /gene="nifS"
                     /locus_tag="ACGI59_001147"
     CDS             complement(17228..18421)
                     /gene="nifS"
                     /locus_tag="ACGI59_001147"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HFG9738950.1"
                     /translation="MEKRRLYMDYSATTPIKKEVLDAMMPYLTDYFGNASSFHTFGRE
                     AKDALDKAREQVAALINAEPSEIYFTAGGSESDNWTLEGVAYANKNKGNHIITSKIEH
                     HAILHTCEYLAKHHGFEITYLDVDSEGKVDLKQLEDSIKDTTILISIMFANNEIGTIQ
                     PIKEISEIAKKHKILFHTDAVQATGNIPVDVKELGIDLMSMSSHKIYGPKGVGALYIR
                     KGVRLHNFVHGGAQEKSKRAGTENIPAIVGYGKAAELAKENMQNHVETLTRLRNKLID
                     GVLERIPYTRVNGSLENRLPGNANFAFQFIEGEGILLLLDMLGIAGSSGSACTSGSLD
                     PSHVLLAIGLPHEIAHGSLRLTVGDFTTDDDIDYILENLPKVIERLRSMSPLYDDAKK
                     QGLVK"
     gene            complement(18439..18885)
                     /locus_tag="ACGI59_001148"
     CDS             complement(18439..18885)
                     /locus_tag="ACGI59_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HFG9738951.1"
                     /translation="MKLSTKGRYGLKAMFELALNQDNGPVSLKFIAKKQKISDQYLEQ
                     IFSSLKKSGLVKSVRGAQGGYLLSKNAEDITVGDILVVLEGPVALSDCVLDEDVCENS
                     NMCVTKIVWEKMKKGIEDVIDSITLKDMINDYNKNKLENDITNIKK"
     gene            complement(18994..19764)
                     /locus_tag="ACGI59_001149"
     CDS             complement(18994..19764)
                     /locus_tag="ACGI59_001149"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFG9738952.1"
                     /translation="MIIKLNSTYHSRVMKYLKKEPEYNLFIIGDIERYGYGNNFLNIW
                     ADVGEHGEIKAILLKYFEFMMFYSDGEYDVEGFYNLLRNTNYEEISGKICAVDALAKR
                     LGLNNLKVVDFCKLQTKKFLIDNNCNAKVKRIRLGNLKKTVKLYDLIDEFHSTTLENL
                     KNGLRTGRGYCIEINKQVVSMAKSTSENRTHAMIIGVGTHPKYRAKGLATKCLIKLCS
                     ELLRENKIPCLFYDNEEAGKIYKKLGFENIGKWGIYSK"
     gene            complement(19795..21060)
                     /locus_tag="ACGI59_001150"
     CDS             complement(19795..21060)
                     /locus_tag="ACGI59_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HFG9738953.1"
                     /translation="MPLADKIRPKTMNDVIGQSHIIGNGKILSKILQSNFLPNMIFFG
                     PPGVGKTTVAEIIAERSNKSFYKINATNSSLEDIKKVISELGSINNVNGVLLYIDEIQ
                     SFNKKQQQSILEFMENGSITLIASTTENPYHYVYKALLSRSTVFEFKPLEKLDIEKGL
                     KRAVEVLNEDSYMDIECNNDAIEDIAILSDGDMRRALNILEVVVYSTKPNKDNITYID
                     SDVIRASTFNKIINYDKNGDSHYDILSAFQKSIRGSDPQASIHYLARLIKGGDLISIC
                     RRLLVIASEDIGLAYPNAIVIVKACVDSAMQLGFPEAKIPLAEATILLATSPKSNSAC
                     IAIMKAMDELDKEFVKDIPNSIKDAHYSGSKEIGRGCGYKYPHNFKNHYVKQQYLPDS
                     IKDSIYYIPQENKTEKNIKKYLEYLNSDF"
     gene            complement(21167..21952)
                     /gene="codY"
                     /locus_tag="ACGI59_001151"
     CDS             complement(21167..21952)
                     /gene="codY"
                     /locus_tag="ACGI59_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HFG9738954.1"
                     /translation="MASEVLQKTRKINKTLQTSGGSSVSFDLLAGALGDVLSSNVYVV
                     SAKGKVLGLHLNDVQDSSVIEDEYTKQKKFSDEYTQNVLKIDETLENLNGEKILEIFP
                     EEHGRLQKYTTVVPILGSGQRLGTLVLSRYSNSFNDDDLVIAEYSATVVGLEILRAIG
                     EELEEEMRKKAVVQMAIGTLSYSELEAVEHIFAELDGKEGLLVASKIADRVGITRSVI
                     VNALRKFESAGVIESRSLGMKGTHIRILNDKLTDELKKLKNNQ"
     gene            complement(22117..24204)
                     /gene="topA"
                     /locus_tag="ACGI59_001152"
     CDS             complement(22117..24204)
                     /gene="topA"
                     /locus_tag="ACGI59_001152"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFG9738955.1"
                     /translation="MAKTLVIVESPAKAKTIEKFLGKSHYTVKASVGHVRDLPKSKLG
                     VDIENNFEPQYINIRGKGDVIKELKKEAKKSKQVYLATDPDREGEAISWHLAHILNLD
                     ESEDCRIEFNEITKDAIKKAIKHPRNIDINLVDAQQARRVLDRLLGYQISPILWQKVR
                     KGLSAGRVQSVTTKLICDREKEIKAFIPKEYWTIDVEAKTVKDKDITLKFYGKNDEKI
                     ELENEEAVNEILKEMEGKDLKVEKIESKSRRKSAPKPFTTSMLQQEGANKLFFTTKKT
                     MMIAQELYEGIDIEGEGTVGLISYIRTDSKRISDEAKEKAKGYIVNNLGENYYKNIGD
                     KKEKKDTKKVQDAHEAIRPTSVDRTPESIKASLSKDQYKLYNLIWRRFVASQMEDSVF
                     DILNVECKIGDIVFKATGSKMKFDGYTKVYNFSEREDKILPSIEKGDILNIENILPDQ
                     HFTQPPARYTEASLVKTLEELGIGRPSTYAPTIATILNREYVEKQGTSLCPTELGIIV
                     TDILENNFHKFIDVDFTADMESKLDVIEEGNTDWKKIVSESYAPLKEAIEEALENIEK
                     VNMDEETDEICENCGSNMVIKYGRFGKFMACKNYPDCKTTRPIMNKIGVKCPKCEEGD
                     IILRKSKRGKAFYGCSNYPNCDFVAWNKPTGEFCEKCGSYMVEKVTKSETKTVCSNKE
                     CNKETKEHNDGKE"
     gene            complement(24237..25337)
                     /gene="dprA"
                     /locus_tag="ACGI59_001153"
     CDS             complement(24237..25337)
                     /gene="dprA"
                     /locus_tag="ACGI59_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFG9738956.1"
                     /translation="MEKRDTYLWLKSIGGITTKTIEIIENEIVNIEDIFDFSEKEIYN
                     LKNISLNIRKNIVKYRGHAYLENIKELLYKKAIKYICKYDKEYPENLKNIYNAPKLLF
                     YKGDIGLVNNNFNIAIVGSRKPTAYGINCAKTISCQLSQYGVNIVSGLAIGIDAYSHI
                     GCMSGKSKTIAVLGSGVDNPLPKQNLHLSNKILENGGLLLSEYNINSTVAPYHFSNRN
                     RIISGLSDGVVVVEAAIKSGALITVDFALEHGKNVFAIPGNINSQMSRGCHKIIKEGA
                     KLIENIDDILNEYNIFNIIDKKINQKYDNISLNAKSKQIIEAIKREGNLHIDSICDYT
                     GIEIKYVNSIINELVLNELVVEMNNKTYSLNV"
     gene            complement(25356..26957)
                     /locus_tag="ACGI59_001154"
     CDS             complement(25356..26957)
                     /locus_tag="ACGI59_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HFG9738957.1"
                     /translation="MLSIINSSNLVGIDSFLVKVEVDVSNGIPSFNIVGLPGKEIKEA
                     RERVKSAILNSGYKFPSTRIVVNLSPADIKKEGAFLDLSISIGLLRELIKKDENYIRE
                     SMFIGELSLDGKIRKVRGILPIIMGAKTQNIKRIFIPIENIKESLFVDEIDIIPIKSL
                     KECVDFLNEEIKVDKVRIMSFLDDKSRKENEELEKDNSYIDCKYTKINNEESKYDEDF
                     KDVKGNYFVKRSAEIAAAGNHNMFMIGPPGSGKTMIAKRVRTILPDISVEEMIEVSKV
                     YSILGMINETKGIIDKRPFRAPHHTTTKQSLIGGGMDARPGEIALAHRGILFLDEIAE
                     FDRKILETLRQPIEDGYVNISRVKYSAKYPCRVLLVAAMNPCPCGYYMSETECRCRSN
                     EIDRYINKISGPLLDRFDIFVEVNSIKYSDFNSLKQEESSQKIKRRVENARKIQINRF
                     KKDNIKNNSEIKAYNLFKYCKLEKEASKTAEMIFSKYNLSSRSYTKLLKMARTIADLE
                     ERDLINSQCIIEAFSFRKAYYSYFK"
     gene            complement(26988..27332)
                     /locus_tag="ACGI59_001155"
     CDS             complement(26988..27332)
                     /locus_tag="ACGI59_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HFG9738958.1"
                     /translation="MNNKEKGDFGEKVAVNYLLSKGAKILEKNYRLKIGEIDIIAKME
                     DEIIFVEVKSRSNIKFGYPCESVSFKKRKKIIGVANYYIIKNNLNNTPIRFDVIEVYL
                     LEKRINHIMNAF"
     gene            27516..28679
                     /locus_tag="ACGI59_001156"
     CDS             27516..28679
                     /locus_tag="ACGI59_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFG9738959.1"
                     /translation="MNREKLVAFIRYLSLFILVVSYLKNVENTLYPVITMIILFLIII
                     NNQVRFFSLSNENKFILISYFLELILIFILCNYTKTFNSIYFVPLILDISFLIKEKYK
                     YILFSFVIISSLIISLDKNIYLALESSSILLIITVLSIYIENENLSKLYSQNLYDKLR
                     ISKDELKKVNADLETYASSIEELAILKERNRISREIHDSVGHSLSTTIIQLNAIEKLL
                     KDKPLIYDLVHELREFVTESFQDVRRAISELKPVEYENYQSLFKIKELVKSFIKLTNI
                     NVKLTISKNTWNLSRNQSIALYRLIQESLSNSSMHGKATEIRIFITFNTSNLIITISD
                     NGIGCGNIKKGNGLNSINERIYELNGKVEFDNSNNGFTIRASFPKFSGGDFFE"
     gene            28672..29319
                     /locus_tag="ACGI59_001157"
     CDS             28672..29319
                     /locus_tag="ACGI59_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFG9738960.1"
                     /translation="MNKIKVLIVDDEKLIRKGLKIILSSYNDLEIVGDASNGYEALEF
                     CKMNDVDIVLMDIRMKVCDGVLGTRLIKEYNNSITLLILTTFNDDEYIKDAMKFGASG
                     YLLKDSSDEVLHEGIRSSFFGNIVLDKSVAEKIMTSEKTIKQEHLYDMYNLTEKEISI
                     IRLIANGLNNKEISQELFLSEGTIKNNITNILAKLELRDRTQLAIFAFKNKIVIE"
     gene            29448..30383
                     /locus_tag="ACGI59_001158"
     CDS             29448..30383
                     /locus_tag="ACGI59_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFG9738961.1"
                     /translation="MDIVRVNNITKRFNDKLVLDNISFSVKKGEIFGLIGPNGAGKST
                     LINIITNLMLPNSGSIQINGLDLSKDYIKAKSIIGLVPQELAIIETLTPFDNLEYFGA
                     FYGLKGKLLKERIIEALEVTGLTEVKKKKVKKLSGGMQRRLNIGIALLNHPKILILDE
                     PTVGVDPQSRNHIFNFIKDISKKHETTVIYTSHYMEEVEHLCSKIFIMDEGKEIAFGN
                     NDYLKSLVSTNTKLIMEIKNINAQLIFDLKNTKGVISVLENNSLLELDIDKKLQLTDI
                     LSVIDKNNSKIMKISYEEPSLEDVFLNLTGKNLRD"
     gene            30397..31494
                     /locus_tag="ACGI59_001159"
     CDS             30397..31494
                     /locus_tag="ACGI59_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9738962.1"
                     /translation="MKLYMSVKTMLKSLKSSFILNLIYFLALPLILSWFLGMVTESMF
                     QNPIKTESTPIVIYDKDNTRLSNDLTKYLKNDLSYILTVKKDDSKAELKLTIPKGYES
                     SLLNEKSNTLNIEKLGTRDDIAILLQDILDTYHEKFYLNNSQKISSEDFSKLFNKNSI
                     DTSIINNNVKQSSYEYFALVSLGFLVIIFIMNNILSNYISESKGLSKRLYSMPITRVQ
                     FLIYDFVGLWIYSFIFLLLYVLFFRILGITFKGNFAILLLLCALSSYFMTSISTFVTS
                     FFSKKYGTAIVYALLFLQTIFGGIFSMISDTFTKLTSLSPTYLIGELFSNYETFKTID
                     SIGNLIFTCLIISTILIALAIVKEKYKWREV"
     gene            31497..32561
                     /locus_tag="ACGI59_001160"
     CDS             31497..32561
                     /locus_tag="ACGI59_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9738963.1"
                     /translation="MIRLTIANIKRYLKNHTLLINMVMLPIILIFSLNFFINNSGNQS
                     MYFSPVAIVSDSSGKYEHKLINSSKLKENSFRLNEQDKAMNLLKNNKVSAVFVLDKNF
                     SNSIDKLQRPVVKCFKIENGGGSLWAESQIESFITKSLKLKVDKNIDDKLTKTNIIDN
                     KPDKNKGSFLVVFLICYFMYINAAHLASDLFTLKKSNVLKRLISTDNKDIKIIFSIFL
                     GLFFIQSIVYTFALLCFSIIEDFNLSLNVLMIVLANSFVSTGFVFWVARVFKNESSIS
                     LISTFYSLIGLAISISSLIPSMDKLSFMTNLSKFTPFYWTIDAIKNNGSILINIIALI
                     LIGIIFVTAGSLKLRDFAKN"
     gene            32805..33302
                     /locus_tag="ACGI59_001161"
     CDS             32805..33302
                     /locus_tag="ACGI59_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HFG9738964.1"
                     /translation="MYKIGESVMYPKEGACSVNDIVTKKINHEMQKYYELSVIFNSNL
                     KISIPVLNADRIGIRPVMDGNDVDNFIQSINKTDGVWIFDRKERLKLYQDKFHSGDVF
                     EIVKLIKMLMIQDSSKQLCSTDKEFLNKAQKFALSELAAAQCKSYTMVLEEMKKHILN
                     SKNTN"
     gene            complement(33478..33726)
                     /locus_tag="ACGI59_001162"
     CDS             complement(33478..33726)
                     /locus_tag="ACGI59_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9738965.1"
                     /translation="MSRKAYEEALVELEKFIDERKEIIKSAEDCIDKYIVDRTLPFDY
                     KDKCVEWQQELLDIAEAQVLEANELSVLLQEKKELEED"
     gene            complement(33792..34808)
                     /locus_tag="ACGI59_001163"
     CDS             complement(33792..34808)
                     /locus_tag="ACGI59_001163"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HFG9738966.1"
                     /translation="MEYNELLKSARENLNGSCKVCKVCNGVACAGEVPGMGGKGSGSS
                     FIENSKSLEKVKVNMRVIHDVSNPDTSIEMFGKKMSAPIFAAPVTGTTLNMGGKINER
                     DYIEPVVAGCVNSGIYAMVGDTAVDAFLMENLDVVKKYNGAGIVFIKPWDNENIIKKI
                     RLAEEAGAFAVGVDIDACGLVTLSLHGKPVLPKNVKQIKELVKSTKLPFILKGIMTVE
                     DALMAVEAGVDAIVVSNHGGRVLDCTPGACEVLPKIADAVKGKVTILADGGVRTGLDV
                     LKMIGLGADAVLIGRPFVTASFGGATDGVETYVNKLQSELSSSMILTGCQTIKDIDGK
                     VIYK"
     gene            complement(34866..35633)
                     /locus_tag="ACGI59_001164"
     CDS             complement(34866..35633)
                     /locus_tag="ACGI59_001164"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFG9738967.1"
                     /translation="MQDKSVREIKEIIETLEVEKYMEYIELLRVDERKSVQGLAIKLA
                     KKLDNIRKEEERLETINIFENEGYDKGYLYIGGIDEAGRGPLAGPVVASVVVFKKDTK
                     IEGVNDSKKLSEAKRDELFEVIKEEALDYGIGIVNNEEIDEFNILNATYMAMKKAINC
                     LKKAPDYLLVDAATIPGIDISQNPIVKGDSKSISIAAASILAKVTRDSIMYQYDRVYP
                     EYGFKSHKGYGTKEHYEAIEKYGITPIHRKSFLKNIL"
     gene            complement(35701..37890)
                     /locus_tag="ACGI59_001165"
     CDS             complement(35701..37890)
                     /locus_tag="ACGI59_001165"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HFG9738968.1"
                     /translation="MKYIIKRDGTKELFSKSKIEDAVYKASINSEGGVDRELAYGISR
                     KIANDYNSMEKCLSVEEIQDIVEVLLMESNRKDIAKHYILYRERRSEIRTKAWEFDEL
                     QKSIWKNKYRHNGESFDEWIKRISNNSSRISKLIRQKKFLFAGRILANRGLQKEGKKV
                     TYSNCYVIEPPKDSIEDIFDTAKKLARTFSYGGGVGIDISKLRPKGAKVNNAAKHTTG
                     AVSFMELYSMTTGLIGQRGRRGALMISMDVNHPEIEDFIDIKTDLEKVTKANISVRVT
                     DEFMQAVREKKLYNCTFEVETTGEKIVKIVNAYDLFNKFVKNNWDYAEPGILFWDKIK
                     TYNFMSEDKNFCFSGVNPCAEEPLPSGGSCLLGSINLSEFIIQPFGEHATFDINKLKS
                     CVREAVVALNDVLDEGLNLHPLQEQRDSVHKYRQIGLGVMGIADMLIKMGIRYGSDES
                     LELCDLIGKTMLNEAVKQSALIAKEYGTFSEYKYENISKSKFFIENLNEDTKEMVKKY
                     GLRNSQLLTIPPTGSISTMIGISGGIEPIFNLSYIRKTESLHDEDVYYKVYTPIVKEY
                     MDKKGITDEECLPDFFVTAMNIGYEERINMQKVWQKYIDASISSTINIPFETTVEDVF
                     NIYIKAWEAGLKGVTIFRDGCKRSGILINEKKEENNKEDKKENSKKEYYKNNKKEKDN
                     KDEKAKEIINKNFEGDEKFVCPECGSEGIAHTGGCSICLKCGYSGCN"
BASE COUNT        12241 a         6601 c         4094 g        15270 t
ORIGIN      
        1 aaattacata ctaaattatt gtgattttat atttttattc atttgttgcc acttcttgat
       61 aattttctat aggtggcaat tcttcaagac tttctatatt caacaacttt aaaaattcat
      121 ctgttgtttt ataaattatt ggtttcccta tcttattcaa tctaccagct tctcttataa
      181 gttcattttc aaacaaagtt tgtaatacct tatcacattt tactcctctt atatcctcta
      241 tttctacctt tgttattggt tgtttgtagg caattatagt aagagtttct aatgtagctt
      301 ggctcaaact tttctttttc tttggttcaa taatcttctt tatatattct gcatagtctt
      361 tatttgtaca catttggtac ttattctcta acttgattat ttgaatacct ctattttgtt
      421 ctctgtactc ctcaataagt aaatttaaca taatctctat ttcttttgat gaaagttctt
      481 tatttattat atagtttaat tcttttatac ttattggttc accatatgca aacataacag
      541 attctataat atactttata tcttctcttt tcattttaac actccgaact ttttcttatt
      601 agaatatcat caaaaaatat atcttgtact acgactattt ctttttcttt tataagctct
      661 aatatagata aaaaagtagc tattatttca tcattttcat atgattttat aatatttgta
      721 aaagtaactt caatcttctc ttttataata tctcttatat ataatatttt ttcttctaca
      781 gatattattt tcttccttac tattttatct agtttctcat cattagcatt ttctatctgt
      841 gatgttttaa ctttaaatat gtatggaagt atatttttta ttgcttctat tgatatatct
      901 tctaaatcca cattgtcatc aataattatc tcttctttat ttctataaaa tctctcatta
      961 acataagtta tattttcttt tatatcttgt gatgctacct taaactttct atattcttct
     1021 aacttttcca taagttctat tcttgggtct tcttcttcat ctttttgctt ataaagcaga
     1081 tatttagact ttatctctaa aagttttgat gccatagtta taaattcact agttatctct
     1141 aaatccattt tatctagcat tttcaaataa tttagatact gcttagttat atctattatg
     1201 gatatatcct ttatatctat tttatgcttt gttattaaat cataaagtaa atctaatggt
     1261 ccctcataga cttgtaattg tatattatac ttcatatcaa tccctcttta atctgtttat
     1321 tatattaaac aaataaatat actattttta gcaacactgc atatattggt gttaatatta
     1381 ttctatacat accagtaaat aaagcaatta ataaaattat ataactcatg ctttcaaatt
     1441 tatacaaata ttcattccat ttatatggta caaatgtaga tataactccc catccatcaa
     1501 gtggaggtat cggaagtaag ttaaaagctg caaatccaat actatataaa aacacatatt
     1561 cagctattgt atttatagca tacatatgca catacttatt gataattaca cacagtatag
     1621 cagtcaaaac attacaaaaa acgcctgcaa gtgaaactat caggtttcct atcttatagt
     1681 ttttaaagtt atttggattt actggtactg gctttgccca ccctatctta aggataaaca
     1741 tagccaaaag acctattggg tcaacatgtc ttacaggatt taatgtcatt ctaccataag
     1801 ctttagctgt atcatctccc aataagtgag ctgaataagc atggccaaac tcatgtattg
     1861 atattgccat agctatacca actaaacttg ctactatttc tcctatattc atacttaaca
     1921 ttaatgtaca tctcctaaat taaattttta atattattat aacaattaaa tagcttataa
     1981 tcaaaattat tttacattat aataatttta acttctaggc ataaaattaa aatttgcttt
     2041 ttattttata acttttatta ttatatcata ttcattgtga aaatataata attacaccta
     2101 ctgtaagtgt aatattatat tgacatgaat tgacattatt gtctcttagt caatattata
     2161 caagaagtat aaaaaaatat gtgttacact ttgtataaca tgaaaggagg ttacctaagt
     2221 gagacaagaa gaacgcacag tatgctttga tagggaatta aaaattgaag catatagatt
     2281 taaaggcata atgcaaaaat tcccaaacca ttttcatgaa cattatgtaa ttggttttat
     2341 agaaaaaggt caaagatatt tatcttgtaa aaataaagag tataccacaa gtactggaga
     2401 tttgctactt tttaaccctt ttgatagtca tacttgtgaa caaatagatg ataaagtact
     2461 tgattacaga tgtattaata ttaagccaga aataatgaaa aaaactgtct ttgaaattac
     2521 aggtaaaaat tatttaccaa aatttaatca acctgttata tttcgcagtg agctagtacc
     2581 tctactacaa gaattacact atataatcat ggaagaagaa ttggatttta aaaaggaaga
     2641 gctttttttc ttcctaattg aacaattaat tgaagaacat acagagccaa atttgcaatc
     2701 taacttggaa aatacaaata tagaaattca agcagtatgt gattatctag aaaataatta
     2761 tgctgaacat atagtacttg acgaattaag cactatagca ggtatgaata agtactctct
     2821 acttcgtaat tttacgaaat taaaaggtat tacaccatat agatacctag aaaatatacg
     2881 ggtaaacaaa gctaaaaagc ttttagaaaa aggcgtagaa cctatagatg ctgcgataca
     2941 aacaggattt gtagaccaaa gtcactttac aaactttttt aagaatttta ttggtcttac
     3001 accaaagcag tatcaaaata tatttataaa tgatgattca aacaacttta aataaggtaa
     3061 aaaggagaaa taaaaatgga aactaaaaat tcaaaatgtg tcatggtaat agacgaaaat
     3121 ttaccaatgg gaattatatc caatacagca gctataatgg gaattacact aggaaaacat
     3181 gccccagaaa ctgttggccc agatgtaatt gacaaaacag gtaatagcca cttaggaatt
     3241 attgacattc ctgtacctat tttaaaagga aacaaagaaa taataaaaga tttacgtaaa
     3301 aaattatata ctttggaatt taacgattta actgttgtag atttttcaga tgtagcacaa
     3361 agttgcaatc tttatgaaga atttacacaa aagattgcta gtgttccaga agatgagtta
     3421 cagtattttg ggatagcaat ttatgggaat aaaaagaaag tgaataaact tactggtagt
     3481 atgcctttac ttagataatt gttaaacaac cctatatatg atattaaatc aaatgatatt
     3541 ttaagataca taaacttttc tatatttcta taaaaatcat aagattataa aatacttcta
     3601 tatggtaaat aaaaataccc ctatggcaaa taattacata agggtatttt ttatgtgtat
     3661 tataatcata tctttgtgta ttataattat atttcatctg ataattattt atatcatatt
     3721 atcaattatt ctttgaagca tttgtagata gcttgcctta tttatatcct tagtatttat
     3781 caattccact tcgcctatta cttttttatc tttacatatc tttatttttc ctaaaacagt
     3841 tcccttttta attggcatct taggattttt tactatttca attttcttct caaattcttt
     3901 agaacttcct tttttaataa gagcatttaa atcttcttta gcaaccaatt taactttgtt
     3961 ttcatcagcc ttatccaatg taagtgttgc tatattatca cctttactac ataacttaac
     4021 actttcataa ttagcaaatc cataatttaa aaggctagag gcatcattaa atctttctgg
     4081 agaagtctct gctcctaaag ttgcagcaac tagatgagta ttacctctct ttgcagatgc
     4141 tgataaacag tattttgctt cttgagtaaa accagtttta acaccagttg ctccttgata
     4201 atgctttata agtttattag tatttgcaag acctactgta acttgtttct tccctactac
     4261 aaccttatcc atccatgtag ttaagtattt acttatcatt tcgtgtttta atagttctct
     4321 agacattaaa gctatatcat gagcagatgt atagtggttt gcaactggaa gtccatttgt
     4381 atttgcaaaa tgagtatcat tcatatttaa ctcttttgct ttagcattca ttctatcaac
     4441 aaatccttca acacttccag ctaagtgttc agccatagca acacaagcat catttgcaga
     4501 agcaacagct atacctttta ataatgtatc aaccttttga gtttctcctg gctctaggaa
     4561 tatttgacta ccacccatac tagatgcagt ttcacttact tgaacttcat catcaagctt
     4621 aatcttgcct gaatccaaag cttctactat aagtagcata gtcattacct tagttacact
     4681 agcaggagga agctttttat gagcatcctt ttcatataaa atttgtccac taccaacatc
     4741 catcaaaatt gctgattttg aagatatatt tagttggttg gcatcagcat tttccttatt
     4801 atttgcaaaa gaaaagctta aaggagtaag cgcaattaat atagccaaag cagtactagc
     4861 taattttttc atcgatgtca cctcttaaag tttatttaaa cttattttct cacatttaac
     4921 tttttttatt ctaaatgcct agatatacct tgacctaaat tttttttctt ttcaagcttt
     4981 ttaaagtaac cttctaaaac acctttatta gacgcatttt ctattatttc aaaagcatct
     5041 tcataattca ttccatactc tcctgctatt tccatttttg tttgttttaa agcttttata
     5101 gcattttgtc ttgaaacatc atccataaga atcctcctat cagtaaaatt tatatataag
     5161 tagtttttac taataaaaga ttgttataat tggtatttta tgtaatatat atttttttaa
     5221 aatgagtatt aaaaagacct cttgtaattc ttaagataat atcaattgga tttctgatat
     5281 tactttaaaa attacaagag gtctaatttt caaatttata gtttttttat attatacttc
     5341 cattatgata ggaagtatca ttggatttcg tttagttttt tgatataaat attcttttag
     5401 gttttcttta atattattct ttaaataagc ccattcttta atatttttct cttcacattc
     5461 atttagaaca ttcttaatta tatcctttgc tccatccatt aaatcttctg actctcttac
     5521 atatacgaat cctctggaaa ttatatctgg acctgctaag accttaccct catccttgga
     5581 aatagtaact acaactatca taagaccatc ttctgataaa tgctttctat cccttaaaac
     5641 tatattacct acatcgccta ctcctagacc atcaactaat atgtttcctg tttgtatacg
     5701 gtttgctacc ttagcagaat ttttatctaa ttctaaaaca tctcctgttt gcatgacaaa
     5761 aatattttct ttatccatac caagctgttc agctatttca gcatgtcttt ttaacattct
     5821 atattcacca tgagctggca taaaaaactt tggtctaact aatgcatgta taagttttaa
     5881 ttcttcttga caggcatgtc cagaaacgtg tatatcagct atatcactgt atactacttc
     5941 tgctcctttt tcaaataaga aattgattac tcttgatatt aatttttcat ttcctggtat
     6001 tggatgagca gatattataa ctaaatcgcc actctttatt tctacttttt tgtgctctga
     6061 aaaagccatt ctagcaagag ctgacatagg ttcaccttgt gaaccagttg ttattatcac
     6121 taattcacta tcttcatact tacatatatc atttaaatct ataagcatgt catcagaaat
     6181 atctaggtat cctaattctt ttgcaactcc aactacattg accattgagc gacctgatat
     6241 agcaactttt ctattaaact tttcagcagt atttattatt tgttgtaatc tgtgtatgtt
     6301 ggatgcaaag gtagcaacta taattcttct gccatttcct tttgcaaata attcgtcaag
     6361 acctacccca acagtctttt cagacatagt aaatcctggt ttttctgcat tagtactatc
     6421 tgccagcatt aaaagaactc cttttttgct caactcacag attctatgca tatccataac
     6481 atctccatca ataggagtca agtcaatctt aaaatctcct gtgtggtata ttataccttg
     6541 gtctgtgtgt attgcaatag agtaagcatc tggtatacta tggttatttt tcaaaaactc
     6601 tacttccata ttatcaagct ttataacttg tctaggacct ataacattta acttaacatt
     6661 attcatttta tgttctttaa gttttacttg tataagacct atactaagtc ttgccccata
     6721 tacaggtaca tttattttct ttaatatata tggaagagct cctatatggt cttcatgtcc
     6781 atgtgtaata aatataccct ttattttatc cctatttttt actaaataag ttatatctgg
     6841 tataactata tctactccta acatttcatc ctctggaaaa ctaagtccag catctataac
     6901 aattatttca tctttatact caacaacagt catatttttt ccaacttcgt taagtccacc
     6961 caaggccatt acctttatct tgttggtgtt ctttttgaac aattgcatca tctccttctg
     7021 tctatttaat tttttaacac gttctacaat cataaataat atcaaatcac gcactcgcta
     7081 ccagttattc ttattgtacc ataattcgta tcatatttta agctatattt aaaatacatt
     7141 taacgtatta ttttgacaaa cctattttat tttgatatcc tctaaatatt atatgattta
     7201 atatcggata atttaggagg atttatgcgt ggtaaaactc attgtgcaat aggaatctta
     7261 acagctatcc aaacatcttt aatatttaaa attcctatat ctttagttga tattttagtt
     7321 tcagctactt ttgctgtact acccgatttg gataaatcta attctatggt ctcaaatttc
     7381 attctaaaaa acaatgtatc caaatacata tatagaattt ttatatatgc tgttaatatt
     7441 attattttct ttatttcaat aaatataaat gataattttt atttaagcgc aataattaca
     7501 tttgttgcaa ttataattat tgaggcaaaa ctaacccaca catttctaag aaaagttttt
     7561 ctatcattaa tatttatact attagctata tgcttataca tcatagaagt tgaaatatat
     7621 tttacaattt tttgtctaat gttgtcaatt tttccctggt taaagcacag aagtttttct
     7681 catagcatat ttgcaactat aatagtatat tttttactaa aacaaataga attaatcact
     7741 aatattaata atttatcttt ttatggtacg attggttatg caagtcatat gtttttagga
     7801 gatttattta caaagcaagg aataccaatt ttttatccgc taagcgaaaa gaaaatttct
     7861 ttaggttttt taactgttgg aggaccattt agtaatttca tagaaaaatc attcattttt
     7921 gtacttatag gattaattat attttcaata ttaaaactat aagcatcttg aaaactgtat
     7981 atttgcattt tatagatggt ttataataaa aaatggaaga atagcataga atataaaagt
     8041 tctatactat tcttccatcg acattaagca atagaagttt attcttctga atcgcttact
     8101 ccattacact cgtcacatag tccataaaat ttaacatcat gatcaagtat tttaaatttg
     8161 tacaatgatt gtatctcttt ttctatattg tctaaaaggt cttctttagc ttccattatc
     8221 tttccacaat ttttacaaat aagatgatga tgattatgac agtcttcttt atgtagacta
     8281 atctcatatc ttatacatcc atcgtctaag tttagttttg atattaatcc tatttcatcc
     8341 aatagctgca ttgttctata tacagtagct aagcctattt caggacagtc aaccctaact
     8401 aaatcataga tttcctcact acttagatgt gaatggtcat gtttaagcaa tatttcaact
     8461 attgctcttc tttgtggcgt aatcttaaaa ccagtttctt ttaacttgtc ttttaataaa
     8521 tccattgtat ttgccataac atcaccctta ctattttcaa ttgataatct ttattaaata
     8581 atattgtaat tgataattca ttttttgtca agacatttaa ataaatttaa tttattctca
     8641 taatataaat atatacccat aaaagatata ttattcacca aaattcataa attttttggt
     8701 atttttaata aaaaacaaaa aaacagatat atactataaa tgcttttaca attaataatg
     8761 tttaattaaa gcaaatataa taaatatctg atttttatat acaaacttaa ttacttatct
     8821 agttaagtcc atttgattca tcttcgtcgt aatttaaacc ttcttcttcc attatagcag
     8881 tatacacagc tacaacagtt tcatactctt catcactttc caaaggtatt aagatttcac
     8941 cttcttcatc ctcatcaatt ctaaagatta aagcttcttc agcttcttca tcatctaaag
     9001 gcattaatat tgcatactct tttccttctg cttctaatgt taatattatt tcaaattggc
     9061 tttcaacgcc attttcgtct attaaattta ttatattttc ttccataatg tcattcacct
     9121 caaatttatt tttattttta ttatttatat tatatatcat aagtattcaa ttttatagtt
     9181 cttttatgaa tttattttaa aatctaaata tccttgcaaa attattactg ctgccaacat
     9241 atctataacc ttttttcttt tttgtctact aacatctgct tctaataaag acctctctgc
     9301 cgctactgta gaaagcctct catcccaaaa ctctacatca atacctgttt ctgcttttac
     9361 caattcacaa aacttgatta ctttttctcc ttgtggacct aaagttccat tcatattttt
     9421 tggtaatcct gatacaattt tatttacttg tttttctttt attattgctt ttagctcttc
     9481 aatatctttt ttcttgccaa ctctttttat tgtttttaca ccttgtgcag ttaatcccat
     9541 caaatcactc acagcaactc caatagtttt atcaccaaca tcaagaccca ttattcttcc
     9601 atctaacata gttcctcctt tatctattta tattctatag attaaataca tattctgaac
     9661 ctctcataac taaggtcaca ggcttactgc ttcatataat tttacataat caatcataaa
     9721 aattgcaata tgatttttta tgtaagcatc cagtttacat taataatcat attgcatttt
     9781 ttatttaaat cctatatgaa cagtaaaaat ttttttagtt atttttttca aatattatca
     9841 tatttatact tattattatt agtggtataa gtatatatat agatgctgta gatgcagcaa
     9901 ctattccaga atcattaact aaaagtgtaa ttatacaacc aaccatagat gcaataaacc
     9961 ctttaaacaa gattggaaaa ttattcatta tctttctaaa gtgtctagat ggtttaaata
    10021 taagcactgc aattactcca attcctacaa gtaaaatatt tacccataca cttgtctgtg
    10081 ctagttgcat attcatttga atttttcttc caaatgtttg taatatctct cctggtccat
    10141 ttaggaaaat tctctgtaca aacataccta aatgagattc actacctgaa agtatatcta
    10201 aagctgcaaa tgcaaaaacg acaactacag ctgaaacagc taataataca gctttcttaa
    10261 aatctatttt aatgtcatat ataagcatta taaacaacaa atatgctaca cattctgata
    10321 tagctccacc aacatttgca cccattgaag gatatgcact agttataagt attattaatg
    10381 aaaaaattac aacaagtaat ttagatacct tcttataatg taataatact gctaagccaa
    10441 atactgcact tgctatagtt acaccttcat attcatttcc tacaccatag tacctagctc
    10501 ctatgacagc atcataactc attatgttat tttgcattaa gtatgttcca aatacagaat
    10561 ctataactat gactaaaata gtaagtgttg caaaaaatcc catgtgcttt atatcattgt
    10621 ccttaaataa ttttctacct aacaagtata atattagcgt agttgcaaat attcccaaag
    10681 atattgaaat tggagtttta aaattcatca aaggagctaa cataaatgat agtggcatta
    10741 ttatgcctaa ttttataaac tccttaagta ctttgaatac acgctcttta tgtggcatat
    10801 aatttctaaa gaaaatcgca aacatggcaa ttacccaaga agcagatatt acgcctacaa
    10861 agccatttat aatagttgac cttattgaag atatagacac tatcctttca taaagtgatt
    10921 ttagatagct aatattgtcc tctttattta ctaaagtata acttctacct accatctctt
    10981 tattttcaag accaaatgta cctagtatat ctactccaac atctaaattg gcaacaattc
    11041 cagcctttct tgttgtggca gattccagta gacctttccc ttctccacta aattttatta
    11101 ttggagaaag tcttctctta ttcttataat ctaggtcact tggaaatgca cttgctatat
    11161 aaacagtgtc attttctcca accatagaaa atacttgttc aagatattta tttatatttt
    11221 ttacaatatt tttcttcatt atatcataag tattatcatt tagatttaat ctatacatat
    11281 ctaatctata tgtatctcca agttctacaa atatagcatc actattttta taggattttt
    11341 tagtttcatc tagtaattta tcataatctg tagatattcc aaatggcatt ttagaatctt
    11401 tcttattaat attatcaata ttacctgaat caatccttcc atattggtcc atagctacta
    11461 aacataagtt tctgttttta actaattgat tattttcaat tatatcagca tttccaatta
    11521 cagatgtctt taaaccattt tctgataaag tttttcctaa tgaccctaaa tttgctccat
    11581 attctccata ctcaagattt tcatttatag atctattaat gcctaaatca tttatttctt
    11641 tagctttttg acctgttgcg acttcaaaag ctttagcagt atcttggtct actttttgaa
    11701 aattgatata gtcttcagta gttacactag ctcttgttcc tgcacccata cttgcataag
    11761 accttctatc atcagtacct tgatcccctc ttatattcat gagagctaca tagcctctct
    11821 tttctatctc atttttcaag attggaatat tcatcatact tccaagattg gtcctattca
    11881 tatcaataaa tatgaccttg cctttttctt cagcaaaacc atagctagca gttgatacaa
    11941 aaatcataat gactattgca aacaacgaca taatcttttt tctcatttat ttgacctact
    12001 ttctattcag atagtgcgtg attacttctt caagtatctc atctctttca aattttttaa
    12061 ttatagctct tgcattttta tgactagtta tatagctaga atcaccagaa aggagatacc
    12121 ctataatttg atttataggg ttatatcctt tttcaactaa tgctttatat acaaaatcaa
    12181 tagtatctcc tacactcatc ctatcttctg gtattccttc aaatttcata gtgtagtcta
    12241 aatctttttc caaactaaaa acctccttga gtgaatgtta tttaacttgt gtcttaataa
    12301 cctcacttgc atagcttaaa gcttggtcaa ccttagatac atcactagct cctgcttgtg
    12361 ccatattagg tcttcctcca ccttttcctc cagctatttt tgcaacttct ctaacaatat
    12421 tacctgaatg tattccctta tctaatacat ctttagtggc agtaactaca aagttaactt
    12481 tatcatctac tacatttgca agtactacta caccagaacc aagcttatct cttagattat
    12541 ctgctgtttc tcttagtgta tccatatcca taccttcaaa tttattagtt atcaaattaa
    12601 ctccatttac ttcaacttta gaatcaaaaa tagaatcaac tgattgtaaa ctcatcttag
    12661 ctttcatatc atgcaattct ttagataagt ttttattttc ttctaataaa gaacttattc
    12721 tttgaattaa attatcttct ttagatttta agtttacaca aacttcagag ataattccat
    12781 ctctttcttt taggtattca taaacagctt ttcctgttat tgcttctatt cttcttacac
    12841 ctgctgctac tccaccttca gataatattt tgaacattcc tacttgagaa gtatttgtca
    12901 agtgagttcc tccacaaagt tctgtagaat aatctcccat agaaactact cttacctcat
    12961 caccatattt ttcaccaaat agtgctgtag ctcctttttc tttagcttcc tttatattca
    13021 taatatcaca ttttatgtct aaagatgata aaataacatt atttactttt tcttctatta
    13081 cttttaattc ttcatttgat atcgcttcaa agtgagtaat atcaaatcta agtctttctg
    13141 gagttactaa agaaccagct tgattaacat gttctcctaa tacttctctt aatgccttat
    13201 gaagtaaatg tgtagcactg tgatttcttg cagctgacat tctagtttct ctatctacga
    13261 gtgtcttcac tttgtcccca ttacttattc ttcctgattt tattatacct atatgtttta
    13321 tactattatt agcacctttt ttagtattta atacttctaa aactaagttt tcattagtta
    13381 tcaatccaca atctccaact tgtcctccac cttcaggata gaaagtagtg ttatctagta
    13441 ctatagatac cttatcgcct tcttctgcac tttgaactag ctcatcatct ttaactatag
    13501 cttcaatagt tccttcacca taaatttcac tatacccatt aaatgtactg tcaactgtag
    13561 attctaattt tgatagaggg tcttccttcc aactttctcc atccatattt cctctagcat
    13621 ttctagctct ttctttttgc ttttccattt cttcattaaa agcttcttca tctacagata
    13681 gatgttcttc ttctaatatt tctttagtta agtctatagg gaagccataa gtatcatata
    13741 gtttaaaagc ttcttgacca cttaggacag tctttccttc attttttaat acttctatat
    13801 aagagttcaa tatttctgtt ccttggtcta tagtttcatt gaatttttct tcttctattc
    13861 caactacttt tttaatataa ctttcttttt caactaaaac tggatatgct ttatcattta
    13921 cttttataac ttcatctatt agtttctgta aaaataattc ttttactcct aataacttac
    13981 catgacgagc agcacgtcta aggagtcttc taagaacata tcctcttcct tcatttgatg
    14041 gaagcactcc atcactaact aagaatgtta cagctcttat atggtcagtt attattctta
    14101 ttgatatatc attttttgga ttctctccat attttactcc tgttaacttc tcaactgctt
    14161 ctagtataga tttaattgta tcaacttcaa agatagtgtc tacgccttgc ataatacatc
    14221 ccattctttc aagacccata cctgtatcta tattcttgtt ttctaataga ctataatttc
    14281 cttcttcatc tctatcaaat tgagtaaata catggttcca aaattctaaa tacctatcac
    14341 aatcacagcc tggtttacag tctggattgt cacatccata ttcttctcct ctatcaaaat
    14401 atatttctga acaaggacca caaggacctg tacctatttc ccagaaatta tcatcttttc
    14461 caagtctaac cattctttct tctgggaaat tcatttcttt tgcccatata tcataagaat
    14521 catcatcttc ttcatataca gtcacccata tcttatcttc tggtatattt agccattctg
    14581 taacaaattc ccatccccat tttattgact ctcttttaaa gtaatctcca aatgagaaat
    14641 tacccatcat ttcaaagaat gtagcatgtc ttgcagttat acctacattt tctatatctc
    14701 ctgttcttat acatttttga gatgtacaca ttctcacact tggaggtact tctacacctg
    14761 aaaagtagtt tttcaaaggt gccatacctg agtttataag taacaaactc ttatcattgt
    14821 ttggtactaa agaataacta tttgctacat aatgaccttt tgactcaaaa aattctaaaa
    14881 acttacttct tatttcattt aatcccatct tttccatatc atgttcctcc taataaattt
    14941 tattttttcg tttaaataaa aaacctcgtc cctataaata aatttatagg ggcgaggaca
    15001 atcgcggtac caccctaatt atcatacaaa gtatgatatc ttcggaaaac cttagctata
    15061 acgtaactac cgtcaatctc tacttaaatc ttctttataa aagataaatt gttcaagatt
    15121 gctactaaaa ggctgcttca aatatcatat tctaagggct tccagcattt ccctctctct
    15181 gtaagacatc aaatatttta ctattcctta tcactgtatt tgttttttaa ttaatatact
    15241 aattatatta ctaaatttta caaaattaat caatactata ttttcatata caataaattt
    15301 attttataaa ctttatttac tcttaatata ctttattgct tattttttta attttttaga
    15361 tgtttttata aacttttctc gattatacct ccacctacta caatatctcc atcatacatt
    15421 actacagatt gacctttagt aatagctctt tgagcttcat caaaaactat tttaatagta
    15481 tcttctccta ctctatgaat agtagctggt gatggttttg ctgaatatct tattttggct
    15541 tgaactctta agggttcttc taacgtatct attgatataa agttaacatc tttagcaata
    15601 agttcctttt taaataaatc ttcattgtct ccaagaacta tggtattatt tattggattt
    15661 atgtctatta caaacattgg ttttccaaaa gtaattccaa gcccttttct ttggcctata
    15721 gtataatata atataccctt atgttttcca agtatattgc ctttagtatc tacaaaaaaa
    15781 ccttcttcaa tacgcttttt agaatgtttt tttacataat ttgcatagtc attatcttta
    15841 acaaaacaaa tttcttgact atcaggctta ttgtgtactg ccatacccat ttccttagca
    15901 atttctctta ctctatcttt ttcatagtca cctattggca ataaagtatg ctctagttgc
    15961 tcttgtgtaa gattataaag tgcataagtt tggtcttttt tatctgtaac agattttttt
    16021 agcaaatatc ttcctgtact ttcatccttt tctatctttg catagtgacc tgtagctaca
    16081 taatcacaac ctatttgtct agccttctta taaaaatcat caaattttat atgtttatta
    16141 caagctatac agggattagg agtttttcct tccaaatatt catctataaa ataatctatt
    16201 actttttctt taaacacttc tctaaaattt aaaacataaa atggtatacc tattttattt
    16261 gctacccttc tagcatcttc aacagcagat aaagagcaac aaccaccttc aatttcaaca
    16321 acatcatcat cttgccacaa cttcatagta acaccaatga catcatatcc ttgttgtttt
    16381 aaaagatatg cagctacaga gctatcaact cctccactca tacctatcat tactttttta
    16441 ttcataaaca aatttcacct catcttttgc attacttatt attataccat atagagcaag
    16501 tattgtactt atttaaaata tagcttgttc tattttctaa aaaaataatc atctttatta
    16561 tttgaatctt atggattatt ttgttaacta attaacctct aaatcaagtt aaaatactta
    16621 atttaggcaa taaaaaatag ctattttaaa aacagctatt ttttatgtcc taattatatc
    16681 attagtactt ctttttaatc attgtaaact tctcataaca caatcactta tttttattct
    16741 tcttcttcaa catcatggtc atgagcatca tctataacat atccatccaa ttctggtata
    16801 tggatattgt tcttttgagc ataatctata agagctgctt ttacagcttg ttcagctaaa
    16861 actgaacaat gcatctttac tggtggtaaa ccatccaaag cttctgcaac agctttattt
    16921 gtaagttcta atgcatcttt tatagttttt ccttttatca tttcagttgc catagaagaa
    16981 cttgctatag ctgaaccaca gccaaatgtt ttaaacttaa catcctttat tacatctcca
    17041 tctatgtcta aaaatatctt cattatatct ccacatgtag ggtttccaac ttcacctaca
    17101 ccacttgcat tatcaatttc tcccatattt cttggattca taaaatgttc cataacttta
    17161 tcactatatt gcattatctt ttcctcctac tcaatttatg tctatttttt atgctttatc
    17221 tttatgtcta ttttactaaa ccttgtttct tcgcatcatc ataaagtggt gacatgcttc
    17281 taagtctttc tattactttt ggtaagttct ctagtatata atctatatca tcatctgttg
    17341 taaaatctcc aactgtaagt cttaatgaac catgtgctat ttcatgtggt aaacctattg
    17401 caagaagtac atgtgaaggg tctaatgaac cagaagtaca tgcagaacca cttgaaccag
    17461 caattcctaa catatctaat aataaaagta ttccttcacc ttctataaat tggaatgcaa
    17521 agtttgcatt tcctggcaat ctattttcta agctaccatt tactcttgtg taaggtattc
    17581 tttctaacac accatctatt aacttatttc taagacgagt taaagtttct acatgatttt
    17641 gcatattttc cttagctaat tctgctgctt ttccatatcc aactattgca ggtatatttt
    17701 ctgtaccagc tcttttgctt ttttcttgtg ctccaccatg gacaaagtta tgaagtctta
    17761 caccttttct tatgtataaa gctcctacac cttttggtcc atatatttta tgtgaagaca
    17821 tactcatcaa atctattcct aattctttaa catctactgg aatattacct gtagcttgta
    17881 ctgcatcagt gtggaataat atcttatgtt ttttagctat ttcggatatt tctttaatag
    17941 gctgtatagt tcctatttca ttatttgcaa acattatact tataagtata gtagtatctt
    18001 ttattgaatc ttctagttgc tttaagtcta cttttccttc gctatcaaca tctaaataag
    18061 ttatttcaaa tccatgatgt tttgctaagt attcacatgt atgaagtatt gcatggtgct
    18121 ctatttttga agttattata tgattacctt tgtttttatt agcataagct acaccctcta
    18181 gtgtccagtt atcactttct gaaccacctg ctgtaaaata tatttcacta ggttcagcat
    18241 ttataagggc tgctacttgt tctcttgctt tatccaaagc atcttttgct tctcttccga
    18301 atgtgtgaaa actagatgca ttaccaaaat aatctgttag gtatggcatc attgcatcca
    18361 atacttcttt tttaataggt gttgttgcag aataatccat atacaatctt cttttttcca
    18421 ttacattcat ctccaaatct attttttaat atttgttata tcattttcta atttattttt
    18481 gttatagtca tttatcatat ctttaagagt aattgaatct ataacatctt ctataccctt
    18541 tttcatcttt tcccaaacta tttttgttac acacatgttt gagttttcac atacatcttc
    18601 atctagtaca cagtcagata atgcaactgg tccttctaat actacaagta tatctcctac
    18661 tgttatgtct tcagcattct ttgaaagcaa ataacctcct tgagcacctc tcacactttt
    18721 tactagaccc gattttttta aagatgaaaa tatttgttct aagtactgat ctgatatttt
    18781 ttgcttctta gcaataaatt ttaatgatac tggtccattg tcttgattta atgctaattc
    18841 aaacattgct tttagtccat atctaccttt agtagacaac ttcatatatt atcacctcta
    18901 tatagaaatc taattcatac tgttttagta ggaattactt tcaagataat tttattatac
    18961 cctactaatt ttgtcaagat taattttaat tttttatttt gaataaattc cccattttcc
    19021 aatgttttca aatcctagtt ttttatatat ctttccagcc tcttcattat cataaaacaa
    19081 acaaggaatt ttgttttctc tcagtaactc tgaacatagt ttaattaggc attttgtagc
    19141 aagtccttta gctctatatt taggatgtgt tcctactcca attatcattg catgagtcct
    19201 attttctgaa gtactctttg ccattgacac cacctgtttg ttaatctcta tacaatatcc
    19261 tctacctgtc cttagtccat tttttaaatt ctctaaagtt gtactgtgaa attcatctat
    19321 taagtcataa agttttactg tcttttttag atttccaagc ctaattctct taacctttgc
    19381 attacaatta ttatctatca aaaacttttt agtttgaagt ttgcaaaaat caactacctt
    19441 taaattatta agtcctaatc tctttgctaa agcatctaca gcacatattt tcccagatat
    19501 ttcttcgtaa ttagtattac gaagtagatt ataaaaacct tcaacatcat actctccatc
    19561 agaataaaac atcataaatt caaaatattt aagtaatatt gcctttattt caccatgttc
    19621 acccacatct gcccatatat ttaaaaaatt atttccatat ccatatcttt caatatcccc
    19681 tattataaac aaattgtact caggttcttt ttttaagtat ttcataactc tactgtgata
    19741 agttgagtta agttttataa tcataatatc tacccccgat acataaagtt cagtctaaaa
    19801 atcactattt agatattcta aatatttttt tatatttttc tcagttttat tttcttgtgg
    19861 tatgtaataa atactatctt ttatgctatc tggtaaatac tgttgcttta cataatgatt
    19921 tttaaaatta tgaggatatt tgtatccaca acccctacct atttctttag aaccagaata
    19981 gtgagcatcc tttatactat ttggtatatc cttaacaaac tctttatcca gttcatccat
    20041 agctttcatt attgctatac atgcagaatt agactttgga gatgtagcca aaagaatagt
    20101 agcttctgct aatggtattt tcgcttctgg aaatccaagt tgcatagctg agtctacaca
    20161 tgcctttaca attacaatag catttggata agctaaacct atatcttcac tagctattac
    20221 taaaagtctc ctacatatac ttatcaaatc cccacctttt ataagtcttg ctagatagtg
    20281 tatagatgcc tgtgggtcac ttcctcttat agatttttga aatgcactta aaatatcata
    20341 atgactatca ccatttttgt catagtttat aattttatta aatgtagatg cccttattac
    20401 atctgaatct atataagtta tattatcctt atttggttta gttgaataga ctaccacttc
    20461 caaaatattt aaggctcttc tcatatctcc atcagaaaga attgctatat cttcaatagc
    20521 atcattatta cactcaatat ccatatagct atcttcattt aatacttcaa cagctctttt
    20581 cagtcctttt tctatatcaa gtttttctaa aggtttaaat tcaaatacag tagacctact
    20641 taaaagagct ttatatacat aatgatatgg attttctgta gtgctagcaa tcaaagttat
    20701 actcccattt tccataaatt ctaatattga ttgttgctgt tttttattaa aactttgtat
    20761 ttcatctata tataatagga ctccatttac attatttata cttccaagct ctgatatcac
    20821 cttttttata tcttctaaag atgaattagt agcatttatt ttatagaagc ttttattgct
    20881 tctttctgct attatttccg ctactgtagt tttaccaaca ccaggaggac caaaaaatat
    20941 catatttggt aaaaaattag attgtaatat cttagacaat atttttccat tacctattat
    21001 atgactttga cctattacat cattcattgt ttttggtctt attttatctg ctaaaggcat
    21061 tttctcactc ctttttaata tacttatcat taccttaata attcgtctaa aaaaggtatc
    21121 tatttgaaat agataccttt tctaatctaa acctataaaa tatagattat tgattgtttt
    21181 ttaatttttt taattcatct gtaagtttgt cattaagtat tcttatatga gtacctttca
    21241 tacctaatga tcttgattct atcacacctg cactctcaaa ttttctaagt gcatttacta
    21301 ttacagacct agttatacca actctatcag ctatcttact tgctacaagt aaaccttctt
    21361 ttccatccaa ttcagcaaaa atatgttcaa ctgcttcaag ctcggagtag gacagagtgc
    21421 ctattgccat ttgaactaca gctttctttc tcatttcttc ttccaattcc tcacctattg
    21481 ctcttaatat ttcaagacca acaacagttg cactgtattc agctattact aaatcatcat
    21541 cattgaatga atttgaatat cttgaaagta ccaatgttcc taatctttga ccgcttccta
    21601 atattggaac tactgtagta tatttttgta atcttccatg ttcttcagga aagatttcta
    21661 atatcttctc accatttaaa ttttctaatg tttcatcaat ttttaacaca ttttgagtat
    21721 attcatctga aaatttcttt tgcttagtat actcatcttc tataactgaa ctgtcttgaa
    21781 catcatttaa atgaagacct agtactttac cttttgcact tactacataa acattagaac
    21841 ttaaaacgtc gcccaatgct ccggccagta aatcaaaaga gacactgctt ccaccacttg
    21901 tttgtaatgt tttatttatt ttccttgttt tttgtaacac ttcacttgcc atttaatcat
    21961 ctcctcatga aatttaggcc ttttaataac cttttatatt ttctaatatt attttcacat
    22021 ttaatgaaaa tttatttttc tattagttgt ttacacttga aagattatca tagattggaa
    22081 cctttttcaa gagaattatg tgattttttt caattttcac tcttttccgt cattatgctc
    22141 ttttgtctct ttattacatt ctttattaga acacactgtt tttgtttcag atttagtcac
    22201 tttttcaacc atatatgaac cacacttctc acaaaactct cctgttggct tattccaagc
    22261 tacaaagtca cagtttggat aattagaaca cccataaaaa gctttacctc ttttagattt
    22321 tctaagtata atatctcctt cttcacattt tggacattta acaccaattt tattcataat
    22381 tggtctagtt gttttacaat cgggatagtt tttacaagcc ataaactttc caaatctacc
    22441 atatttgata accatattag aaccacagtt ctcgcaaatc tcatcagttt cttcatccat
    22501 atttactttt tctatatttt caagagcttc ttcaatagcc tctttaagtg gtgcatatga
    22561 ttctgataca atctttttcc aatctgtatt gccttcttct ataacatcaa gtttactttc
    22621 catatcagca gtaaaatcta catctataaa tttatggaaa ttattttcca aaatatcagt
    22681 aactattatt ccaagttctg ttggacatag acttgttcct tgcttttcaa catactctct
    22741 attcagtata gtagctattg ttggagcata cgtacttggt ctacctatac ctagttcttc
    22801 tagtgttttt actaaactag cttctgtata tcttgctggt ggttgtgtaa aatgttggtc
    22861 tggcaatata ttttctatat ttaatatatc tcctttttct atacttggta aaattttgtc
    22921 ttctctttct gaaaaattat ataccttggt ataaccatca aatttcattt ttgaaccagt
    22981 tgccttaaat acaatatcgc ctattttaca ttctacattt agtatatcaa acactgaatc
    23041 ttccatttga ctagctacaa atcttctcca aattaaattg tataacttgt actggtcttt
    23101 gctcaatgag gcttttatac tctcaggagt tctatcaact gaggttggtc ttattgcctc
    23161 atgggcatct tgaacctttt tagtatcctt tttttctttt ttatcaccaa tgtttttata
    23221 gtagttttct cctaaattgt ttacgatata acccttagct ttttcttttg cttcatcaga
    23281 tattctttta gagtcagttc ttatatatga tataagacca acagtacctt caccttctat
    23341 gtcgatacct tcatataatt cttgtgctat catcattgtt tttttagtag taaaaaataa
    23401 cttatttgct ccttcttgtt gaagcatact tgttgtaaat ggttttggag ctgatttacg
    23461 tcttgattta gattctatct tctcaacctt taaatcttta ccttccattt cttttagtat
    23521 ttcatttaca gcttcttcat tttctaattc aatcttttca tcatttttac cataaaattt
    23581 caaagttata tctttgtctt ttactgtctt agcctcaaca tctattgtcc agtattcttt
    23641 tggaataaat gcctttattt ctttttctct atcacaaatt aactttgttg taacagattg
    23701 cactctacct gcactaagac cttttctaac cttttgccat aatataggac ttatttgata
    23761 acctaaaagt ctgtctagca ctcttcttgc ctgttgtgca tcaaccaaat ttatatcaat
    23821 atttctagga tgtttaattg cttttttaat tgcatcctta gttatctcat taaactctat
    23881 tctacaatcc tcagattcat ctaaatttag tatatgagct aaatgccatg atatagcttc
    23941 accttctcta tcagggtcag ttgctagata tacttgttta gattttttag cttctttttt
    24001 taattctttt attacatcgc cttttcctct tatatttata tattgaggtt caaaattatt
    24061 ttctatatct acacctaatt tacttttagg taaatctctg acatgaccaa ctgatgcttt
    24121 aactgtgtaa tgacttttcc ctaaaaattt ttctatagtt ttagcttttg caggcgattc
    24181 tacgatgact aatgttttgg ccataaaacc atacaccccc actacctaat cttaatttat
    24241 acatttaaac tgtacgtttt attattcatt tctactacta actcatttag tactaattca
    24301 ttgattattg aatttacata ctttatttct ataccagtat aatcacaaat actatctata
    24361 tgcaagtttc cttctctttt tatagcttca attatttgct tagacttggc atttagactt
    24421 atattatcat atttttgatt tattttttta tcaataatat taaatatgtt atattcattt
    24481 aaaatatcat ctatattttc aattaatttt gcaccttctt ttataatttt atgacaacct
    24541 ctactcattt gagaatttat attaccaggt attgcaaaaa cattttttcc atgttcaaga
    24601 gcaaaatcta ctgtgattag tgcaccactt tttattgctg cttctacaac aactactcca
    24661 tcacttaaac cactgataat tctatttcta ttagaaaaat ggtacggggc tactgtcgag
    24721 ttaatattat actctgataa taataatccc ccattttcta atattttatt tgataaatgt
    24781 aaattttgtt ttggaagagg attgtctact cctgaaccaa gtactgctat agttttagat
    24841 tttccactca tacaacctat gtgcgagtat gcatctattc ctatagctaa tccacttact
    24901 atattaactc catattggga tagttgacaa cttattgttt ttgcacaatt aattccatag
    24961 gctgtaggtt ttcttgaacc tactatagct atattaaaat tattattaac taagcctatg
    25021 tcacctttgt agaataataa ctttggagcg ttgtatatat tctttaaatt ttcaggatat
    25081 tctttatcat atttacaaat atatttaatc gcttttttgt aaagtagttc ttttatattt
    25141 tctaaataag cgtgacctct atattttact atattttttc taatattcaa gcttatattt
    25201 tttaaattat atatttcttt ttcagaaaaa tcaaatatat cttcaatatt tactatttca
    25261 ttttctatta tttcaattgt ttttgttgtt attccaccaa ttgattttaa ccataaataa
    25321 gtatctcttt tttccataaa atccacctca aaaaactatt taaaataact ataataagcc
    25381 tttctaaatg aaaaagcttc tataatacac tgtgaattta tcaaatctct ttcttctaaa
    25441 tctgctattg ttcttgccat ttttaaaagt tttgtataac ttctactgct taggttgtat
    25501 ttactaaata tcatctcagc cgtttttgaa gcttcttttt ccagtttaca atatttaaat
    25561 aaattgtaag ctttaatttc actattattt tttatattat ctttcttaaa tctatttatc
    25621 tgtatttttc tagcattttc tactcttctc tttatttttt gagagctttc ttcttgtttt
    25681 aaagaattaa aatctgaata ttttatagaa ttaacttcta caaaaatatc aaacctatct
    25741 aacaatggac ctgatatttt atttatgtat ctatcaattt cgttacttct acatctacac
    25801 tctgtctctg acatataata accacatgga catggattca tagctgcaac taacagtact
    25861 ctacaaggat attttgcact gtactttact ctggatatat ttacatatcc atcttctata
    25921 ggttgtctta aagtctcaag tatttttcta tcaaattcgg ctatctcatc taaaaataag
    25981 atacctctat gtgctaaagc aatttctcca ggtcttgcat ccattcctcc tccaattaaa
    26041 gattgctttg tagttgtatg atgaggtgct ctaaaaggtc ttttatcaat tattccttta
    26101 gtttcattta tcatacccaa tatactatag accttactta cttctatcat ttcttctaca
    26161 cttatatctg gtaaaattgt ccttactctt tttgcaatca tagtttttcc tgagcctgga
    26221 ggtccaatca taaacatatt atgatttcca gcagcagcaa tttctgcact cctctttaca
    26281 aaataatttc cttttacatc tttaaagtct tcatcatatt tactttcttc attattaatc
    26341 ttagtatatt tgcaatctat ataactatta tctttttcta attcttcgtt ctcttttctt
    26401 gacttatcat ccaaaaaaga cattatcctt actttatcga cttttatctc ttcgtttaaa
    26461 aaatcgacac attcttttaa acttttaata ggaattatat caatttcatc tacaaataga
    26521 ctttctttta tgttctcaat tggtataaaa atacgtttta tgttctgagt ttttgcaccc
    26581 ataattatgg gcaatatacc tcttactttt cttattttcc catctaatga taattctcct
    26641 ataaacatac tttctcttat gtaattttca tcttttttta ttaactctct taaaaggcct
    26701 atacttatag ataaatctaa aaaagctcct tcttttttta tatctgctgg tgataaattt
    26761 acaactattc tcgtgcttgg aaatttatat ccactattaa gaattgctga ctttactctt
    26821 tctcttgctt ctttaatttc tttgcctgga agaccaacaa tattaaaact aggtattcca
    26881 ttgcttacat ctacttcaac cttaactaaa aagctgtcga ttcctactaa gttgcttgaa
    26941 ttaataatac ttaacatatt taatcctttc caaaaacatt atctttttta aaaagcattc
    27001 attatgtgat ttattctttt ttctaataaa tatacctcta taacatcaaa tcttatggga
    27061 gtgttattta agttattttt tataatataa taatttgcaa ctccaattat cttttttctt
    27121 tttttgaagc ttacagattc acaagggtag ccaaacttta tgttagacct tgatttcacc
    27181 tccacaaata taatttcatc ttccattttt gctattatat ctatttcccc tatctttaat
    27241 ctgtaatttt tttctagtat ctttgctcct tttgatagca aataattaac tgctactttt
    27301 tctccaaaat cacctttttc tttattattc attattcact ccaatacatt aaattttcac
    27361 ttttaaatta tggtcagtat acaattttac tattcaagtt tttggattga ttttatttcc
    27421 atcaatatta ataaatactt ctattgactt tattctttta tgatattatt ctagttttca
    27481 taggaaaagt ttattttttt ggaggagtgc ctttaatgaa cagagaaaaa ttagttgctt
    27541 ttatacgata tttatcttta tttatattag ttgtaagcta tttgaaaaat gttgaaaata
    27601 cattgtatcc ggtcataact atgataatat tatttctaat tataataaat aatcaagtta
    27661 gatttttttc tttatcaaat gagaataaat ttatacttat ttcttacttt ttagaactta
    27721 tattaatttt tatactttgt aattatacaa aaacttttaa ttcgatttac tttgttcctt
    27781 taatattaga tatatcattt ttaataaagg aaaaatataa gtacatattg ttctcttttg
    27841 ttataatatc ttctcttatt attagtttag ataaaaatat atatttggca ctggagtcaa
    27901 gctctatatt gctaattata actgtacttt ctatatatat agaaaatgag aatttatcaa
    27961 aattatacag tcaaaatctt tatgataagc ttagaatttc taaagatgaa ttaaaaaaag
    28021 ttaatgctga tttggaaact tatgcaagtt ctatagaaga attagcaatt ttaaaagaaa
    28081 gaaatcgaat atcaagagaa atacatgata gtgtaggaca ttctctttct acaacaataa
    28141 tacaattaaa tgctatagaa aaactcttaa aagacaaacc tctaatttat gacttagtac
    28201 atgaacttag agaatttgta actgaaagtt ttcaagatgt aagaagggct atatctgagt
    28261 taaaacctgt tgaatatgaa aattaccaaa gtttatttaa aatcaaagaa ttagttaaaa
    28321 gctttattaa gcttacaaat attaatgtta aattaactat ttcaaaaaat acttggaact
    28381 tatccagaaa tcaatctata gctttatata gacttataca ggaatccctt tccaactcat
    28441 cgatgcatgg aaaggcaact gaaatcagaa tttttattac attcaatact tcaaatctta
    28501 taattactat tagtgataat ggtataggat gtggcaatat aaaaaaagga aatggactta
    28561 atagtatcaa tgagagaata tatgaattaa atggtaaagt tgaatttgat aattctaata
    28621 atggtttcac aattagagct tcttttccta aattttcagg gggtgatttt tttgaataaa
    28681 attaaagttc ttatagtaga tgacgaaaaa ttaattagaa aaggtcttaa aattatatta
    28741 tcatcttata atgatttaga aattgtagga gatgcttcta atggatatga agctttagaa
    28801 ttttgtaaaa tgaatgatgt agacatagtt cttatggata tacgaatgaa agtatgtgat
    28861 ggggttctag gtacaaggct tataaaggaa tataataatt ctataacatt attaatttta
    28921 acaactttta atgatgatga atatataaaa gatgctatga agtttggtgc ttcaggatac
    28981 cttttaaaag atagctctga tgaagtattg catgaaggga ttcgttcttc attttttggc
    29041 aatattgtgc ttgataaaag tgtagctgaa aaaatcatga cctctgaaaa aacgattaaa
    29101 caagagcatc tttatgacat gtacaattta acagaaaaag aaatatctat aattagacta
    29161 attgcaaatg gacttaataa taaagaaata agtcaagaac tattcttatc tgaaggtaca
    29221 ataaagaata acataactaa tattttagct aaactagaac ttcgtgacag aactcaacta
    29281 gcaatctttg ctttcaaaaa taaaattgta atagaataaa tctaaaaata taaagtgact
    29341 taagtcactt tttttagtga cttatagtaa tagaattata attcacaatt ctttattata
    29401 atattatagc tttttaactt atacaaaatt attttggagg gattactatg gatatagtca
    29461 gagtaaataa tataacaaaa agatttaatg ataaattagt acttgataac atcagctttt
    29521 ctgttaaaaa aggtgagata tttggactta ttggcccaaa tggtgcaggt aaatctacac
    29581 ttataaatat aattacaaat cttatgttac caaatagtgg tagtattcaa atcaatggtt
    29641 tagatttatc aaaagattat ataaaagcaa agtctataat agggttagtt ccacaagaat
    29701 tagcaattat agaaactctt accccttttg ataacttaga gtactttgga gctttctacg
    29761 gtttaaaggg aaaactactt aaagaaagaa tcattgaagc attagaagtt acaggtctta
    29821 ctgaagtcaa aaagaaaaag gtcaaaaaat tatctggtgg tatgcaaagg agattaaata
    29881 ttggaatagc actcttaaat catcctaaaa tattaatatt ggatgaacca actgtaggtg
    29941 tagaccccca atcaagaaat catattttca attttataaa agatatatct aaaaaacatg
    30001 aaactactgt aatatatact tcccattata tggaagaagt tgagcacctt tgttctaaaa
    30061 tttttattat ggatgaaggt aaagaaattg cttttggaaa taatgattat ttaaaatcac
    30121 tcgtatctac aaatacaaaa ttaattatgg aaataaaaaa tataaatgcc caacttatct
    30181 ttgatttaaa aaatactaaa ggtgttatat ctgttttaga aaataattca ttattagaat
    30241 tggatataga taaaaaatta caacttacag atatactctc tgtcattgac aaaaacaatt
    30301 caaaaataat gaaaatttct tatgaagaac ctagcctaga agacgtattt ttaaatctaa
    30361 caggaaaaaa tttaagggac taatggaggt gattttatga aattatacat gagtgtaaaa
    30421 actatgctaa agagtttaaa aagctccttt atacttaatt taatatattt tttagctttg
    30481 cctctgattt tatcttggtt tcttgggatg gttacagaaa gcatgtttca aaaccctata
    30541 aaaacagaat ctacccccat tgttatatat gacaaagata acacaagatt atcaaatgat
    30601 ttgacaaagt atttgaaaaa tgatttatct tatatattaa cagttaaaaa agatgattct
    30661 aaggcagaat taaagcttac tatacctaaa ggctatgaga gtagtttatt aaatgaaaaa
    30721 tcaaatactc taaacataga aaaacttgga actcgtgatg atattgcaat attgcttcaa
    30781 gatatattag atacttacca tgaaaaattt tatttaaata attctcagaa aataagttca
    30841 gaagattttt ctaaattatt taataaaaat tctattgata cttctataat aaataacaat
    30901 gttaaacaga gctcttatga atactttgct ttagtttcac taggatttct tgtaataatt
    30961 tttataatga acaatatttt gtcaaattat ataagtgaat caaagggact ttcaaaaaga
    31021 ctttattcta tgcctataac tagagtacaa ttcttaattt atgactttgt aggtttatgg
    31081 atttattcat ttatattctt attattatat gttttattct ttagaatact tggaataaca
    31141 tttaaaggta attttgccat attgctccta ctttgtgctt tatcatctta ttttatgact
    31201 tcaatatcta cctttgtaac cagctttttt agtaaaaaat acggtacagc aatagtttat
    31261 gcattactct ttttacagac aatttttgga ggtatcttca gtatgataag tgatacattt
    31321 actaagctta caagtttatc tcctacctat ttaataggag aattgtttag taattatgaa
    31381 acatttaaaa ccatagattc aataggaaat ttaattttca catgtttaat tatctctaca
    31441 atattaattg cacttgctat tgtaaaggaa aaatataaat ggagggaggt ttaataatga
    31501 ttagattaac tattgcaaat ataaaaagat atctaaaaaa tcatactctt ttaattaata
    31561 tggttatgct tcctattata ctaatttttt cacttaattt ttttatcaac aactcgggaa
    31621 atcaatcaat gtacttttct ccagtagcaa ttgtatctga ctcttcagga aagtatgaac
    31681 ataagttaat aaatagttct aaattaaaag aaaattcatt tagattaaat gaacaagata
    31741 aggctatgaa tctattaaaa aataataagg tttctgctgt attcgtttta gataaaaatt
    31801 tttcaaatag tatagataaa ttacaacgac ctgtagttaa atgctttaaa attgaaaatg
    31861 gtggtggtag tctttgggca gaatcacaaa tagaatcttt tattactaaa tcgttaaaat
    31921 tgaaagtgga taaaaatata gatgataaat tgacaaagac aaatataatt gataacaaac
    31981 cagataagaa taaaggttca tttttagtag tcttcttgat atgctatttt atgtatatta
    32041 atgcggcaca tcttgcttct gatttattta cattaaaaaa atcaaatgtg ctaaaaagac
    32101 ttatctctac agataataaa gatataaaaa taattttcag tatattttta ggactatttt
    32161 ttattcaatc aattgtatac acttttgctc tattatgttt ttcaattata gaagacttta
    32221 atttgagtct gaatgtattg atgattgtac tagcaaatag ttttgtttcc actggatttg
    32281 tattttgggt agctagagta tttaaaaatg aaagttctat aagtcttata tctacatttt
    32341 attctttaat aggtctagct atatctatct ctagtctaat accatcaatg gataaattat
    32401 cctttatgac taatctttca aagttcacac cattttattg gactatagat gctattaaaa
    32461 acaatggtag tattttaata aatataatag ctctaatatt aattggaatt atatttgtaa
    32521 ctgcaggaag tcttaaacta agagattttg ctaaaaacta ataattatta aatataatta
    32581 gtatctattt ttatataatt gtaaaagaat atttcaacat attaaattat tttatcttat
    32641 atttttctct ttagtttcac acatcaccaa gtctaaactc tatattatat attaatattt
    32701 ttgacttgtc aagttattta tgttataata ttaatcatta gataattaaa gcattgtacc
    32761 taagttgcaa tatctaaaat tcaatttgga ggtataacta gcatatgtat aaaattggtg
    32821 aaagtgtaat gtatccaaaa gagggagcct gctctgtaaa tgatattgta acaaagaaaa
    32881 taaatcatga gatgcaaaaa tactatgaat taagtgtcat ttttaatagt aaccttaaaa
    32941 tttcgattcc agttctaaat gcagatagaa ttggcatacg tccagttatg gatggaaatg
    33001 atgttgataa ttttatccaa tctattaata aaacagatgg tgtatggatt tttgatagaa
    33061 aagaaagact taaactgtac caagataaat ttcattcagg agatgttttt gaaattgtaa
    33121 aattaatcaa aatgcttatg atacaagaca gttcaaaaca attgtgtagc acagataagg
    33181 agtttttaaa taaagctcaa aaatttgctt tatcagaatt agctgctgct caatgtaaat
    33241 cctatacaat ggtattagag gaaatgaaaa aacatatatt aaattcaaag aatacaaatt
    33301 aaaccagttt tttaaactgg ttttattttt tatactaatc atccatgcta ctttagctta
    33361 ttttaaactg cctaatctaa tattaataaa gttatctcaa ttagatagat gtattttatt
    33421 caaataaaaa aaactaagag taaatcatat tactgattta ctcttagcta ataaaattta
    33481 atcttcttca agttcttttt tctcttgtaa taatacactt aattcatttg cttctaaaac
    33541 ttgtgcctct gcaatatcaa gtaactcttg ttgccattcc acacatttat ctttgtaatc
    33601 aaaaggtagc gttctgtcaa ctatgtactt atcaatacag tcctccgcac ttttaattat
    33661 ttcttttctt tcatcaataa atttttctaa ttctactaag gcttcttcat aagcttttct
    33721 actcatataa attccccctt tgtacaatta ttctacatta aaatgatttt tcctcttttt
    33781 tgtataaaat tttatttgta tataactttt ccatcaatat cttttatagt ttgacatcca
    33841 gttaatatca tagaactact tagctctgat tgaagtttat ttacataagt ttctacacca
    33901 tcagttgcac caccaaaaga agcagtcaca aaaggtcttc ctattaatac tgcatctgca
    33961 cctaaaccta tcatttttag tacgtctaaa ccagttctca ctccaccatc tgcaagtatt
    34021 gtaactttac ctttaacagc atcagcaatt tttggtaaaa cttcacatgc tcctggtgta
    34081 cagtcaagca ctcttcctcc atgatttgat acgactattg catccacacc tgcttctact
    34141 gccattaaag catcttcaac agtcattata cctttcaata taaatggtag tttagtagac
    34201 tttactaact cctttatttg tttaacattt ttaggaagta ctggttttcc atgtaatgat
    34261 aaagttacta gaccacaggc atctatatca actccaactg caaatgcacc agcttcttca
    34321 gctaatctta tcttttttat aatattttca ttatcccaag gtttaataaa tacaattcca
    34381 gctccattat actttttgac tacatctaaa ttttccatta aaaaagcatc tactgccgta
    34441 tcaccaacca ttgcataaat accactatta acacatcctg caactactgg ttctatgtaa
    34501 tctctttcat taatttttcc acccatattt aaagtagttc ctgttactgg agctgcaaat
    34561 ataggtgcac tcattttctt tccaaacatc tctatagatg tatcaggatt tgatacatca
    34621 tgtattactc tcatatttac ttttactttt tctaaactct tgctattttc tataaaagaa
    34681 gaaccacttc ctttacctcc catgccaggc acttcacctg cacaagcaac cccattacaa
    34741 accttacaaa ctttacaact tccatttaaa ttttctctag cactttttaa taattcatta
    34801 tattccatct ttaatccccc attaactata gtaatttata ttatatacat tatatcatat
    34861 tatatttaca gtatattttt caaaaaactt tttctgtgga taggagttat gccatatttt
    34921 tcaatagctt catagtgttc tttagttcca tatcctttgt gagatttaaa cccatattct
    34981 gggtatactc tatcatattg gtacattata ctatccctag taactttagc aagtatactt
    35041 gctgctgcta ttgatataga ttttgaatct cctttcacaa ttggattttg agatatgtca
    35101 atacctggta ttgttgctgc atcaactaat aaataatccg gtgctttttt taaacaattt
    35161 atagcctttt tcattgccat atatgtagca tttaatatat taaattcatc tatttcttca
    35221 ttgtttacta tacctattcc ataatctagt gcttcttctt ttattacttc aaaaagttca
    35281 tctctttttg cctcactcag tttcttagag tcatttacac cttctatttt ggtatctttt
    35341 ttaaatacta caacagaagc tacaacaggt ccagcaagtg gtcctcttcc cgcctcatca
    35401 attcctccaa tatataaata acctttatca tagccttcgt tctcaaatat atttatagtt
    35461 tccaatcttt cttcttcttt tctaatatta tccagctttt ttgccagttt tatagctaga
    35521 ccttgtacag attttctttc atcaactctt aacagctcta tatattccat atatttttct
    35581 acttctaaag tctctattat ttctttaatc tccctcacac ttttatcctg cataccattc
    35641 cctcatcttt cgtttttaaa aatttaattt ttaaatatat taaaattttt taattagagt
    35701 ttaattgcat ccactatagc cacattttaa acatatacta catccacctg tatgtgcaat
    35761 tccttcactt ccacactcag ggcacacaaa tttttcatct ccttcaaaat ttttatttat
    35821 tatttctttg gctttttcat ctttattatc tttttctttt ttattatttt tataatattc
    35881 ttttttacta ttttcttttt tatcttcttt attattttct tccttttttt catttattaa
    35941 aattccactc cttttacatc catctctaaa tattgttact cctttaagac cagcctccca
    36001 tgccttaata tatatattga aaacatcttc aactgtagtt tcaaaaggta tatttattgt
    36061 agatgatata gaagcatcaa tatacttttg ccatactttt tgcatgttta ttctttcttc
    36121 atatcctata ttcatagcag taacaaaaaa atctggtaaa cattcttcat ctgtaatacc
    36181 tttcttgtcc atatactctt taacaattgg agtatatact ttataataca catcctcatc
    36241 atgcaagctt tcagtttttc ttatatatga aagattaaat ataggttcta ttccaccact
    36301 gatacctatc attgtagata tggaacctgt aggaggtatg gttaaaagtt gtgaatttct
    36361 aagcccatac tttttaacca tttccttagt gtcttcattt agattttcta taaaaaactt
    36421 agatttagaa atattttcat atttatactc actaaatgtt ccatattctt tagcaattaa
    36481 tgctgattgc ttcacagctt catttaacat agtcttacca attaaatcac atagttctaa
    36541 agattcatca gaaccatatc ttattcccat ttttataagc atatcagcta tacccattac
    36601 acctaaacct atctgtctat acttatgtac actatctctt tgttcttgta atggatgcaa
    36661 gttcaatccc tcatctaaga catcatttaa agctactaca gcttctctaa cgcaagattt
    36721 taatttatta atatcaaaag tagcatgttc accaaatggt tgtattataa actctgacaa
    36781 gtttattgag ccaagtagac aacttcctcc acttggaagt ggttcttctg cacaaggatt
    36841 tactcctgaa aagcaaaagt tcttatcttc actcataaaa ttatatgttt ttattttatc
    36901 ccaaaacaat atacctggtt ctgcataatc ccagttattt ttcacaaatt tattaaataa
    36961 atcataagca tttactatct taactatttt ttctccagta gtttcaactt caaaagtaca
    37021 attatataac tttttttctc ttacagcttg cataaattca tctgttactc ttacagaaat
    37081 atttgcttta gtaaccttct ccaaatctgt ttttatatct ataaagtctt ctatctctgg
    37141 atgatttaca tccatagaaa tcataagagc tcctcttctc cctctttgac caattaagcc
    37201 agttgtcata ctgtaaagtt ccataaaaga gactgctcca gtcgtatgtt ttgcagcatt
    37261 gttaactttc gctccttttg gtcttagctt tgaaatatca attccaactc ctccaccata
    37321 actaaaagtt ctagcaagtt tttttgcagt atcaaatata tcttctatgc tatcttttgg
    37381 tggttctatt acataacaat tagaatatgt tacttttttt ccctctttct gtaatcctct
    37441 atttgctaaa attcttccag caaatagaaa ttttttttgt cttattagtt ttgatattct
    37501 tgaactatta tttgaaattc tctttatcca ttcatcaaaa ctctcaccat tatgtctata
    37561 cttgttcttc catattgatt tttgtaattc atcaaactcc caagctttag tccttatttc
    37621 agagcgtctt tctctatata atatgtagtg ttttgctata tcttttctat ttgattccat
    37681 aagtagtact tcaactatat cttgtatttc ttctacggat aagcattttt ccatagaatt
    37741 atagtcatta gctatttttc ttgatatacc atatgccaac tctctatcta ctccaccttc
    37801 actatttata ctagctttat agacagcatc ttcaatctta cttttactaa aaagttcttt
    37861 tgtaccatct ctttttatta tgtatttcat ttttaaacct ccaataaatt agtattaaat
    37921 taatacacac attaactata actctaaaca aactttttat atatttgtat attttcatat
    37981 taattatcta acataatata tattatacta ttttatattc aatataacac tccttatagt
    38041 tttcttaact tgtaaaatat cttcctaaaa aactcaaaaa taatataaaa aagtatataa
    38101 aagtcaaatt atactcttat atactttatc tttttatatt tttttcattt taactttgat
    38161 taattaataa gtatttagaa aataaattct ttattttttc tttaca
//
LOCUS       DAYAPA010000025        37915 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.025, whole genome shotgun sequence.
ACCESSION   DAYAPA010000025 DAYAPA010000000
VERSION     DAYAPA010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 37915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37915
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.025"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(50..937)
                     /locus_tag="ACGI59_001166"
     CDS             complement(50..937)
                     /locus_tag="ACGI59_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFG9738969.1"
                     /translation="MNIKLELYKVFNAVVNNKSFSLAAKELFMSQPAVSQSIKQLEEQ
                     LDTLLFYRNNKGVKLTPEGKILSEHVTTALKLISSGEDRINKFKKLEYGSLKIGVGDT
                     AARFFLLKYLEIFHKKYPHIHVSTINRTSRELISLLKDGNIDIAIINMPIEDDTLNIV
                     ECIEIHDIFVCANDYIEYKGRKISLEELNTLPLIMLENKANSRLYVNEYFLSKGIKLN
                     PDIELGSHELLLEFAYINLGVSCVIEEFSIDYLENEKLFKLDIKEPIPKRNIGYCHLK
                     DISLSLATKEFLSMISNNI"
     gene            1056..2255
                     /locus_tag="ACGI59_001167"
     CDS             1056..2255
                     /locus_tag="ACGI59_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003493023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HFG9738970.1"
                     /translation="MDRLVGTVSRGVRAPIIRQGDDLVKIVVDSVLNASKSENFEVRD
                     KDVIAVTEAVVARAQGNYAHVDNIAKDVKDKFGDDTVGVIFPILSRNRFAICLKGIAK
                     GCRKVVLMLSYPSDEVGNHLISIDELDDKGINPWSDVLTEEKYRELFGYNKHTFTGVD
                     YVDYYKNLIKDCGAEVEIVFANNPKTILNYTTSILTCDIHTRQRTKRILRQNGAKKVY
                     SLDDIMTASVDGSGYNDQYGLLGSNKATEETVKLFPINCDEVVNKIQGNIKEITGKDV
                     EVMVYGDGAFKDPVGKIWELADPVVSPAYTKGLEGTPNEVKLKYLADNDFADLSGDEL
                     KEAISKYIVEKDNKSDDLTGNMVSQGTTPRRLTDLIGSLADLTSGSGDKGTPIIYIQG
                     YFDNYTK"
     gene            complement(2449..5004)
                     /locus_tag="ACGI59_001168"
     CDS             complement(2449..5004)
                     /locus_tag="ACGI59_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFG9738971.1"
                     /translation="MMSLVKFSIQYIKHYKKQSISIVLSIVLSVALLTGIGSLVHSAD
                     KSRIEKIREDSGDYHYFYKVDKEQLNKIKKNKKSKEYTVDRLGVTKPKNQIDEPFIIE
                     FLNADSSYLDMTGRKLLKGTLPSKDGEIVLDTYSLNNLNVNKEIGTEINLDGKIYKLC
                     GILSSLSNPETALEGFVNDYFQPKEKDSYIVYVKYDENKNIKKQNYKFMKKFNISKEK
                     TYINWPLSVEFGVKPPKNIEKYNIFEFLKNLELNTNAIILLVGVFGAAAVYSIFHVSI
                     LQRISQYGVLEVLGANNKQLLFLLFLELFLLFIVGFPIGCFLGIEVASTIYEQFPHIF
                     LSSDIVPGAFFISEKSIYLGFLFILLLLILIAVRVVHQLNKYSSIESMKNFKITNKKK
                     RILESVKHSNMTKVLSHKYMTQKKGMFIGILFSLSLGGIIFLCSSYSIQLTKNNNELT
                     MKADDGLNSDYQITMQTTDFDVGIPKDKISRLNNVEGISKVYPVRYLFGAMQIKEEQL
                     FWKNFFKPLEKDYRIKTFFDGICTKQNEGGYLLKTNIYGYNNGMLEGLNSYIIDGEIN
                     THDMVKNNKVIVRLPMDGTGMYDAVDINPGDTIKVKVPKTMKPTDEIVKFKEENKNDY
                     TTKEFVVAATVKRVMANNIYFIGDYGMDIVMTNGQMKNNFDIENYNSVSIKKTEDSNS
                     EELSENIKSVVSDVKRCIVTDYTIAIEKNNTYLNQKLLFIYGIVFVLLAISLFHIINT
                     VSYLIFSRRHEFGILRAMGITDNKFLLMMIREGFLYGFYASIIMVIGSVIGQFMIYFM
                     VKRVYLYINPILKINTPLYIGMIILNITISIVAVIIPVRQILKSDIISEINKN"
     gene            complement(5001..5687)
                     /locus_tag="ACGI59_001169"
     CDS             complement(5001..5687)
                     /locus_tag="ACGI59_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9738972.1"
                     /translation="MSILVTNNLVKHYGKGETKVKALNGVSIEIERDTFTAIVGTSGS
                     GKSTLLNIIGGLDNPTSGDVIIKGKNISKIGKKDLTVFRRRNIGFIFQNYSLMPVLNT
                     YDNIALPVTLDKGNHIDHEYIEMLMKTLGIWDKRLKFPSELSGGQQQRVAIARALANK
                     PALILADEPTGNLDSKTTMEVVCLLKESSAKFHQTILMVTHNENIAQVCDSIIHIEDG
                     VVVNTGGEVL"
     gene            complement(5764..6672)
                     /locus_tag="ACGI59_001170"
     CDS             complement(5764..6672)
                     /locus_tag="ACGI59_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFG9738973.1"
                     /translation="MFILAFIICTVVIFIVSTKINRKRYDELNLMLNQILEGKEVTYP
                     DTKDTRASKISHQVKKIKDMIEIEVEQSKSEKEAIKGLISNMSHQLKTPLSNITIYCE
                     LLENINISTLQKKEFLQKMKNETFKIDWLLQSLFKMTKLEDGVIEFEVEELLIKDTLI
                     QSISTIFNKAEAKNIRINLEPFSDIKLVHNKKWTIEAIVNVLENAIKYSPSDSTITIS
                     VIKMELYTKITIKDEGIGIDSRELNDIFKRFYRSKNVENQNGTGIGLYLTRLILEKEN
                     GNIIVESKLGSGCCFSIFLQNCKSLN"
     gene            complement(6674..7345)
                     /locus_tag="ACGI59_001171"
     CDS             complement(6674..7345)
                     /locus_tag="ACGI59_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFG9738974.1"
                     /translation="MNLLIIEDDINLNEGLFYAFENDGFNVFKAYTKQEGLNIFNSKN
                     IDFIILDCNLPDGDGFDVCQIIREKSDIPIIMLTARDSEIDEVKGLEIGLDDYITKPF
                     SLSVLKARVKVAIRKKSNNKVIYSNGIKLDQKLLKVYKNKECLELSSVEYKLLSYLIE
                     NKGQILLKEQILHHIWDSEENYVDDNIVSVNIRRLRVKVEDDPSNPKYIKTAYGMGYL
                     WNEVE"
     gene            7607..8671
                     /locus_tag="ACGI59_001172"
     CDS             7607..8671
                     /locus_tag="ACGI59_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reductive dehalogenase domain-containing
                     protein"
                     /protein_id="HFG9738975.1"
                     /translation="MKRVDERNTMFARANYKKDSTAYNDYYKKNPDKKEIDDSIRNRP
                     NLCSEGTMTYNELNSPMASSAFDFLSDIKSLCEGKVSDTKVDVDAKSMTKKIKGLAKQ
                     YGASVVGITKLKDYHIYTNRGRHEENYGEEINLTHKYAIVFGCEMDKEMINRAPMICE
                     VIETSKCYVDASIVGMILSYYIRNLGYDARNHMDANYLVMPVFIARDAGLGDIGRNAI
                     LTNKDYGSRLRLGVVTTDIPLLEDEYVDFGLEDFCKVCKKCSFNCPSHSLSNDIKIGD
                     DGKYNWVIEHETCYIKWRYLGTDCGMCISACPFSQNLETIKNTTSFKGNNELIQKALD
                     EYTSKFGKRIFIPGNPSWLK"
     gene            8829..9515
                     /locus_tag="ACGI59_001173"
     CDS             8829..9515
                     /locus_tag="ACGI59_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFG9738976.1"
                     /translation="MEKILVVEDDSILNKTLSYNLIEDGYIITSKFTAKSALKSIFEC
                     EFDLIILDINLPDKSGFELCNEIKENYNIPIIFLTANDMECDMIKGYELGALDYITKP
                     FNINIFKQKIKAFLNHLTIKTKQDYYRDGYLEINFSELSANINGNQIIFTPLEYRTLK
                     LLTENPKSILTRKVLLEKLWDIDANFVDEHTLTSVISRIRSKIEKDNLKYIKTVYGMG
                     YMWLGERNEF"
     gene            9505..10536
                     /locus_tag="ACGI59_001174"
     CDS             9505..10536
                     /locus_tag="ACGI59_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFG9738977.1"
                     /translation="MNFKKFSFSSIYLIIAVGIIFTAILFSISIYLITDDLKLIALLL
                     IYTIVLCGFIALIIFLLHKKIVIFCSRICHTLDSMMNSEDNIEIELEEETILSRINHR
                     LIRMYEVIQENRNSIAIEKADLQELVSDISHQIKTPIANLKMINTTLLNQTFKTEIQK
                     DFLMDMENQLDKLDFLMQSMVKTSRLETGIITLSKKKNSIYETIATALSGILFDAEKK
                     NIEVTVDCDPNLYIIHDKKWTSEAIFNILDNAVKYTSSNGKIRVATECWVMYTKIDII
                     DNGKGISESRQAEIFKRFYREEDVHDIEGIGIGLYLSRKIISLQGGYIKVTSDIGKGS
                     TFSIFLPNK"
     gene            10618..11283
                     /locus_tag="ACGI59_001175"
     CDS             10618..11283
                     /locus_tag="ACGI59_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9738978.1"
                     /translation="MSILQVINLKKYYGQSSNITKALDGVTLSIEKGEFISIVGTSGS
                     GKSTLLNMMGGLDNPTSGKVIIKGKELSTMSDEQLTIFRRRNIGFIFQNYNLVPLLNV
                     YENIILPIGLDGETIDKDFINEIINMLSLEEKLYDIPSNLSGGQQQRVAIARALVTKP
                     AIILADEPTGNLDSRTSSDVLSLLKVTSKKFNQTIVMITHNNEIAQLTDRIIRIEDGK
                     IVN"
     gene            11296..13713
                     /locus_tag="ACGI59_001176"
     CDS             11296..13713
                     /locus_tag="ACGI59_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFG9738979.1"
                     /translation="MFRNNNRTFIRKIALNDLKINKLKTYLSGIIIMISTCLLLTVTL
                     VSYNASVDLVNASPYHAIYKSVDEKAKNILYKDKNFSGVGTYKLIGSNKKTDCIMSIV
                     YADDTAIKLMNFQPLKGKLPTNKNEIAISEKYLQEFGLDKDIGDSIKLNYYDDITNKE
                     VQCDFIIVGFLENYYQDNAKQYYTVVSNDYFKNIASTPLQNKSNTFDESRPDTVDVLV
                     KLSEENTEKDSASIKTQLNKIGLSLGIKKYNIYLNDNYIESNLIDGEQIITVLVVGIV
                     VLFSSVFVIYSIFYISVVNSVQMYAKLKSLGMTSFQLKKIISLQGNILSIIFIPLGVI
                     ASCIIAYIIQPLAWQMIADLFIILILSLVMFLTVRISLFKPARIISKISAIEAMQYTE
                     TKFRKKAKNFSYINIKNLALKNIESNRKKNLIALISLSISGVLFISIANLANSIDFKK
                     QLSQQFVYNEDYIISIKSDNLYEHITEIQQNNPLTDSLKNEIKSIHGVKKLIESKSIK
                     SNIIEPYIKDRDEKQFITLIKGITPELSKSLEEYIVSGKIDYTHLDSDSLIINKYRTK
                     YYGLNLKVGDTVTFNIFSGNDIIKKKMKIIGIIDNSNTGGMFFASDKTLESLTPYNTN
                     LDFSILIDKKHSKEVEEKLLSIIQKNSNLMLYSYEDDYRMITRAFQYIIIASYIFVGL
                     ISCFGILNMTNTLINSVLIRKKEFALLQAVGMTRKQLRNMLYREGLNISIKAICTSSI
                     LGYFGSNLLCTFIKDVIRLDFINFKFSIFTILIFSFVLIGIQVLVTELLVRNIEKKSL
                     TERLRSQ"
     gene            complement(13894..14685)
                     /locus_tag="ACGI59_001177"
     CDS             complement(13894..14685)
                     /locus_tag="ACGI59_001177"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008425 -
                     2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0102208 - 2-polyprenyl-6-hydroxyphenol
                     methylase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFG9738980.1"
                     /translation="METKQYLIDFYNTYDEDSRLALKHGMVEFLTTMHYIDKYIKSGD
                     CVLEIGAATGRYSHTLARQGYDVDAVELVEHNIEVFHKNTQSNENISITQGNAMDLSV
                     FPDNKYDITLLLGPLYHLYNKEDKQQALHEAIRVTKPGGVVFAAYVISDGCLIDEGFH
                     RGNINVSEYIEKGLIDPQTFAAKSEPKDLFELVRKENIDDLMSAFNVTRLHYVASDGL
                     ALYMREAVDSMDDDAFALYLKYHLATCEREDLVGVTSHAIDIFRK"
     gene            complement(14718..15305)
                     /locus_tag="ACGI59_001178"
     CDS             complement(14718..15305)
                     /locus_tag="ACGI59_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFG9738981.1"
                     /translation="MPPKVKITKEMILNTVLDITREVGFEAVNARSIASKLQCSTRPI
                     FTCYENMNELKNEFLAFAYEYYNQYVVNYRNSKSVSPYLLLPLSYIEFAQEETYLFKL
                     LFINDLDLMMKEANDFYKEIDNEKKARLFSESIGIDLECAKVIFLDLFLYTHGIAVLT
                     ATKKLTLDRYSAEKMLKNILTAFIRQEKSDWNLSI"
     gene            complement(15482..16873)
                     /locus_tag="ACGI59_001179"
     CDS             complement(15482..16873)
                     /locus_tag="ACGI59_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate/H(+) symporter BenE family transporter"
                     /protein_id="HFG9738982.1"
                     /translation="MERLARREQPHIKFGMFKIRIPFIHYRFEKPEAIQGIISSMTSL
                     GTIGLSTQILGLPYEVAWSMAIINSILYCLHVFMGDPVVPGWITASITLTTAYLLKFS
                     MGIERIQALTALQIDLGIIFILMGITGVAGKLVSKIPNSIKGGILMGVGISTIISEFN
                     PKGRFDSYPISITVGILVACFVMFSERFDTLKVKNKFLFHLGEYGVVSAILVSLIVGI
                     FSKEIEIPRFSFDNLVYIVDFKNLINTVSPFGIGFPSVMLFIQGIPMAFMIYIIAFGD
                     FITGENLVLSESENRKDEYIDFNSNRSNVISGIRNIFMAIFSPYIPMCGPLAATLTGS
                     VAQRYKVGKEAMQSVFSGMGTLVWVSAIFICFYPIAQIATPLIPLALSVTLLVQGYLC
                     TKLSMELCNTGVEKGLIGLMGGVIAAKGGTWGLAVGFILYFILIDSKKRKEKDINSIE
                     DCVYEILDKKKSI"
     gene            complement(17057..18361)
                     /locus_tag="ACGI59_001180"
     CDS             complement(17057..18361)
                     /locus_tag="ACGI59_001180"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase/bile acid-CoA:amino acid
                     N-acyltransferase family protein"
                     /protein_id="HFG9738983.1"
                     /translation="MKVNVYNVESFVDERLKIMVSDLIPNTKLKISVKMEFPWCKGEE
                     FSSYGVFCSNEKGEVDLDLVEPIEGTYKADNCMGLIYSLEKSKTEGKNFAENISIDKP
                     IIMNMIFETSIERKEVELKRIFKSEDITTKYISDEFIGKLFYKQNSNNKTILMLGGSD
                     GNLDALDLLAAPLASRGFNVLTVAYFALEGLPDKLEEVPLEYFEKVFEWISENEITST
                     KEIFVHGTSKGGELAFLLASRYEQIKKVVVSQPHIYCFQALNGLMSGNDTSSWSYKGE
                     PFPYIKVDNDIFFEEQKKNISKGIPFGFNSTYKKSLKRAENRKEARIKIENSKADILM
                     IAGKEDNIWNSYEACLKALEIFEENNYPYNVELLTYNNMGHPLPIPYIMPLKETLCMS
                     MSGGIFSSGGTVEGNSKGQFESWNRTIEFYKKPLSSDLNNYY"
     gene            complement(18720..19595)
                     /locus_tag="ACGI59_001181"
     CDS             complement(18720..19595)
                     /locus_tag="ACGI59_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HFG9738984.1"
                     /translation="MITFDVNGAIIRFDDTKNNYNKIRKIFKLYALEISKNFERDCLN
                     SFQNLKQISDRGLSLGEKYIEESFKKGIETIVSFGIITIDINTFKSVYCEKYLDFKRL
                     FNNLNKTFIIPNKNKKNGHASFLEIESTIKKMSNYLYNDCFKIHYAVVDALIENRITA
                     VHSYIDEESIKKSNALFNNYKDGFIGKADECKVVKQIITLNPYREDVYEFLVKEDGDF
                     SKEIERLTEFLGYDIREYKASLMDIYINELMESDENDVENAKEKIEKYARYIGCSDEN
                     LYVARVNAIYTFANA"
     gene            complement(19799..20485)
                     /locus_tag="ACGI59_001182"
     CDS             complement(19799..20485)
                     /locus_tag="ACGI59_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFG9738985.1"
                     /translation="MFGVIANSLAIAGGCIVGLIIKGGLPQRVSDTIMNGIALCVLYI
                     GISGALEGKNTLVTIISVAVGALIGELIDIDKWVNKLGAFLQSKFSKGNKKDSIAEGF
                     ISSSLLFCIGAMAVVGSLESGLTGSHDTLFVKSVIDGIASIIFTASLGIGVMFSAISV
                     FLYEGIICVGASFLNGFLSDPVVTEMTAAGSLLIIGLGLNVLKLTNIKVANLLPAIFI
                     PILFGVFGII"
     gene            complement(20534..21067)
                     /locus_tag="ACGI59_001183"
     CDS             complement(20534..21067)
                     /locus_tag="ACGI59_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HFG9738986.1"
                     /translation="MSDLNNKKEQDENESSMNETKIYDILDKLNVEYDVVEHEAVYTA
                     EQLVILDGITKGCQCKNLFLRNAKGNKYYLIVVKGEKQVDLNSLKDKIGSSRLSFASP
                     ERLYNVLKLLPGSVNPFSLINDTERKVELYIDKDVIKEDYLNFHPNINTKTVNISRKG
                     FEEFLEYLKYPIQSIDV"
     gene            complement(21111..21743)
                     /locus_tag="ACGI59_001184"
     CDS             complement(21111..21743)
                     /locus_tag="ACGI59_001184"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HFG9738987.1"
                     /translation="MKVFDLTHVTHNDMPVYAEPNRPDIKKVAIIEENGYQETLISVF
                     SHNGTHMDSPRHMYTKGETLDKLDIENFVGKAYVLELEKGNENIELEYLKKYEDEIKN
                     SDFIIFKSGWSKFWDKKQYYVGYPTLTKEAANYIANTNIKGIGIDMLSVDRYDTSVFE
                     VHHILFEKGKIIIENLTNLENVPEKFLFIAAPFKYNDADGAPVRAIAIVE"
     gene            complement(21833..22912)
                     /locus_tag="ACGI59_001185"
     CDS             complement(21833..22912)
                     /locus_tag="ACGI59_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HFG9738988.1"
                     /translation="MKIAIFLGAGASAAENLPIQNELFSEYFKKLNPTNYNTKMNREL
                     YIFFKQMFNIDIVKDNIDNANFPTFEEVLGLLDLAEQRKEAFRNFGLENLNNRSDSIR
                     FLRQYLILLMAEAIHNTPRTDNKYHKLLVENLLENDLLLDTTFISANYDIHIDNTVTG
                     LRDKNDLPIMLDYGVEFTNFKEELNWRKPKEPLIKLYKIHGSLNWLYCPICNSVTLTP
                     HEGGVMKLIENSSETKCLECGELTEPIIVPPTYFKNMSNIFLSDVWNETEKTLRDTDL
                     LIFCGYSFPEADMHIKYMLKRVQTNRKKPPLKIMVFNNHSQKQRITLKKEEGRYKRFL
                     GEDVIFTDNSFQDFSVNPLRFIKNI"
     gene            complement(23045..24391)
                     /gene="pepV"
                     /locus_tag="ACGI59_001186"
     CDS             complement(23045..24391)
                     /gene="pepV"
                     /locus_tag="ACGI59_001186"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HFG9738989.1"
                     /translation="MKEQIKEKVNSLQDEMISSIQESVKIPSVISEATENCPFGENVD
                     KALRGILDLCKSLGFKTVYKDGYYGYAEIGQGEKMIGILGHVDVVPEGDLESWNYPPF
                     EAVLEDGKLYGRGTQDDKGPTISAIYAVKALMDLNVDFNKRIRFIFGADEENLWRCIN
                     KYKENNEEIPNYGFTPDSRFPITNAEKGLLQVHLTCDSKSDIELSVGKALNAVPGKAI
                     YIGKYSDKLKKELNKLNFEYTVEGNKICIIGKSVHSAASDTGINAVARLCIALNNIGI
                     DSNIIKFLAEVIGEDANGNNIIPNCKDDVSGKLTVNIGRVTIDNEKEFAGIDVRIPVT
                     YKKDDFVKELKKMTDKYNLNYEEYDFLDSIYVPEDTLLVKTLRKVYEEETGLDGTPLS
                     SGGATYARALDNCVAFGAIFPGKPETEHQANEYLIVEDIIKATQIYALSIYELLKI"
     gene            complement(24460..25740)
                     /locus_tag="ACGI59_001187"
     CDS             complement(24460..25740)
                     /locus_tag="ACGI59_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFG9738990.1"
                     /translation="MLNETKELLKDYYGIDDDTFKLSQEIMEEIKDKFEEIKEIREYN
                     QYKVLKAMQESKLSDMHFNWTTGYGYNDIGREKIEEIYSKVFNTEDALVRPIIVNGTH
                     ALTLCIQGIVRPGDEILSVTGRPYDTLEGVIGIREEKGSLKEYGVTYDDVDFLEDGNL
                     DLEGIKNKINDRTKLVMIQRSKGYSWRKSLSISDIKEAIEVIKSVKPEAIVMVDNCYG
                     EFLDTKEPTDVGADVMAGSLIKNPGGGLALTGGYIAGRKDLIELISYRMTSPGIGKEC
                     GLTFGTTRNVLQGFFLAPYIVSQAVMGAIFCSRAFEKLGYDVLPKYDDLRSDIIQCIR
                     LNNADEVISFCEGIQEAAPVDSYVKPVPWDMPGYESEVIMAAGAFIQGSSIELSADAP
                     IRPPYNVYFQGGLTFDHSKMGTLKAIEFIKKLKK"
     gene            complement(25867..26127)
                     /gene="hfq"
                     /locus_tag="ACGI59_001188"
     CDS             complement(25867..26127)
                     /gene="hfq"
                     /locus_tag="ACGI59_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HFG9738991.1"
                     /translation="MKNTVLNLQDLFLNNARKERIPVTIYLVNGVQVKGLVKGFDSYI
                     ILIEGDNRQQNMIYKHAVSTIQPGKYINLTNQNQNNNNNNNR"
     gene            complement(26241..27176)
                     /gene="miaA"
                     /locus_tag="ACGI59_001189"
     CDS             complement(26241..27176)
                     /gene="miaA"
                     /locus_tag="ACGI59_001189"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFG9738992.1"
                     /translation="MKKIPLIILTGPTAVGKTDLSIKLAKDMNAEIISADSMQIYEYM
                     DIGSAKVTEEEMQGVKHYLIDEVKPDYPFSVSEFQQRAKKYIHEINKKEKCVLVTGGT
                     GLYLNSLIYNMDFAQSDANNELREELQKQLAEKGIDYMHNKLKELDEESANRIHKNNT
                     KRVIRALEVCLSGKKMNDFSSDLKFNEEYQPIIIVLNRDREHLYQRINMRVDIMLKNG
                     LVEEVKKLLSMGFKKDMISMQGIGYKEILKYLDGEYTYEEAIEIIKRDSRRYAKRQIT
                     WFKRYKTAKWFDLDQYENIDELKNEIILYIKDSIK"
     gene            complement(27214..29181)
                     /gene="mutL"
                     /locus_tag="ACGI59_001190"
     CDS             complement(27214..29181)
                     /gene="mutL"
                     /locus_tag="ACGI59_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFG9738993.1"
                     /translation="MKNIINILDDLTINKIAAGEVVERPSSVVKELIENSIDAGANKI
                     SIDIIDGGKSLIKITDNGIGIPSSEVEKSFLRHATSKIKKIDDLYDLYSLGFRGEALA
                     SISAVSKLEMTTKTKDEIIGTKIYVEGGKIISKEPIGSTNGTTIIIKDIFFNTPARQK
                     FLKSTHAETINISDLINKLAIGNPNIQFKYTNNNKQMLNTPGDGKLVNTIRSIYGKEI
                     TENIIDVEFKCNHFKMNGYIGNNNIYRSNKNLQHIYINKRFVKSKIIIDAITESYKSI
                     IPIGKHAVCFLNIEVDPSCIDVNIHPNKLEIKFEKEQEVYIELRDFLKVKLIHSNLIG
                     KYATYSDKKTQPRIAINSREKSTDYKLRNNDLLESTPKNSNITKGKDEVIEVVTLSSE
                     KPINEFQSVSEVLNASVEDDVKNINYLSEDSANDNIQEEFQVDGIKNEGNYYLGDSIK
                     DSEEEYLCSSKRKFSLYGYSVIGVVFNTYIILSKDDSMYLLDQHAAHERILYERYMEK
                     FYRQDINMQILLDPVVIEVSNVDMLQIENNLELFMKFGFELEIFGNNHIMVRCVPTIF
                     GVPETEKFILQIIDNIEEITSNYDLKGERFASMACRSAIKANDKIYDIEIKSLLEQLE
                     KCENPFTCPHGRPIMVEISKTEIEKMFKRIM"
     gene            complement(29192..32035)
                     /gene="mutS"
                     /locus_tag="ACGI59_001191"
     CDS             complement(29192..32035)
                     /gene="mutS"
                     /locus_tag="ACGI59_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFG9738994.1"
                     /translation="MQIDMNKLTPMMKQYLEVKNRYKDCILFFRLGDFYEMFFEDALV
                     ASKALEIALTGKACGLEERAPMCGVPFHSANSYISKLVENGYKVAIGEQMEDPSTAKG
                     IVRREVIRVITPGTVLDGNLLENKKNNYLLSLYKDGTNIGLTYVDISTGETNATCLNE
                     DKVIEEIAKIHPTEIIINDLDFIENLRDIATVSNIYINESFSDNYLDINILKEYFPDV
                     YLQKLKFDDKGLIKSSLSILLNYIYNTQKQITSNINNINIYNSSEYMVLDMFTRTNLE
                     LTQTIRGNKKKGSLLHVLDKTSTAMGGRLLRKYVEEPLINKSKIENRLDVIEEIKDDF
                     ILREDLNDILKNIYDIERICGKIAFERVTPKELIHLKNSIEKLPNLKDTINLSNAKIL
                     KEYVSEMDKLDDIYNLIDEAILEEPTITIKDGNIIKSDFSDELKELREISKNGAFLVK
                     EIENREREKTGVKSLKIGFNKVFGYYIEITKANFKQAKLDETYIRKQTLSNAERYITP
                     ELKEIEEKILHAEEKIKSLEYEIFVEIRDTIYKNIDRIQKVAKTIANIDVFVSLATVA
                     HINNYVKPAINENNKLDIRNGRHPVVENIVGEENFVPNDTYLNRGENIINIITGPNMS
                     GKSTYMRQTAIIALMAHIGSFVPAESADIPILDRIFTRVGASDDLSQGQSTFMVEMNE
                     VSLILKNATERSLVILDEIGRGTSTYDGISLAWSIVEYIQKNIRCKTLFATHYHELTD
                     LEEEFKEVKNYSIAVKEDGEGIIFLRKIIPQGADKSYGIYVAKLAKLPDEVIERAKYI
                     LKDLEKNHVYNSVAINGDKENNNIINSNLDEELVKVNQSNFKNKYESLKIEHELIVKD
                     YKHIKKDYDKLNSKFKALNDEVALIKQNDDKEKINQVDSVKEVALTQISFDSVNRDIL
                     SEEILNLDILNMTPLDAINSLYNLQRKAKEIKS"
     gene            complement(32195..32929)
                     /locus_tag="ACGI59_001192"
     CDS             complement(32195..32929)
                     /locus_tag="ACGI59_001192"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9738995.1"
                     /translation="MLTQMNFYHNKKVKVSIENVSKTYKDIEVLKDISIDVYEGEFVS
                     ILGPSGCGKSTIFNIITKLTDYDSGKVNINGKYSYMYQKDLLLPYKTIIDNVSLPLIL
                     KKEKKSKARSMVKPYFEVFGLSGYEDKYPSELSGGMRQRANFLRTFINSNDIMLLDEP
                     FGALDSLTKTSMQKWLLDVKKKVNSTILLITHDIDEAIMLSNRIYVISKKPSIVKKEF
                     VIDSRKLNEDNLENIIKLKKEIISLL"
     gene            complement(32898..33911)
                     /locus_tag="ACGI59_001193"
     CDS             complement(32898..33911)
                     /locus_tag="ACGI59_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFG9738996.1"
                     /translation="MNFKKLGILGLVSVFTLATITGCSSNNNKDKNNDKDTSSKKVTM
                     VLDWTPNTNHTGLFVALDKGYYKEEGLDVEIVQPPESGAETLVATGKADFGISYQEQV
                     TYAKTSEDPLPIKAVATVIQHNTSGFASPKEKNITTAKDFEGKTYGGWGSPSEEAVFK
                     AVMKKNGADFNKLKIVNTGQDDFFAAMKTVDFAWIFEGWDAVKADLIGYDLNFIPVKD
                     LDERLDYYTPLIISNETVLKDNPELAKKFLKATTKGYEYAIKNPEESAKILVKHAPEV
                     DEKLALKSQEYLASKYKDDAPRWGEMKDSVWNNYTSFLKEYKLIDKDMKASDAYTNEF
                     LPQ"
     gene            complement(33933..34703)
                     /locus_tag="ACGI59_001194"
     CDS             complement(33933..34703)
                     /locus_tag="ACGI59_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9738997.1"
                     /translation="MKNLEIIDKVKDSIYPIATFLFILILWQSMVGVLEVPQYILPTP
                     VDIINVFFKDYQNLSMHAVVTIGEAILGFIVAIIISLVIGILMDFVSIIKKCLYPIML
                     VTQMIPTIIIAPLFMIWFGFGTMPKVLMVMLTCFFPILISFVDGIENIDKDYLNLFKT
                     MDSNKVNTFIHLKFPMAMDKFFSGLKISATYAVMAATVAEWLGGTKGLGVYMLRSKSA
                     YALDKVFASTILVVIFSLMFVGIVQAVKKVVIRHRLID"
     gene            complement(34678..34968)
                     /locus_tag="ACGI59_001195"
     CDS             complement(34678..34968)
                     /locus_tag="ACGI59_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HFG9738998.1"
                     /translation="MVIADIAVMPLRPYKGEEEMYKVVDACIAEIEKSGLKCEIGAMS
                     TTVEGDFDEVFELLKKVHKIPFNLGCERVITVARVDEKAGGLTIDEKLRNHR"
     regulatory      complement(35099..35198)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     regulatory      35819..35908
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            35961..36137
                     /locus_tag="ACGI59_001196"
     CDS             35961..36137
                     /locus_tag="ACGI59_001196"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9738999.1"
                     /translation="MFMDKMDRCTHILTAYICSSRDYCNFIDTQLNDFILEYGENVVE
                     SCLHQVMVLVSRYN"
     gene            complement(36252..36608)
                     /locus_tag="ACGI59_001197"
     CDS             complement(36252..36608)
                     /locus_tag="ACGI59_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFG9739000.1"
                     /translation="MDIDNNFEEFPNIEERQKDFNCSIGFTMTVIGSKWRAIILWHIL
                     KQEPIRYSQLKKEVVHISHKILSQELKYLERDGLIKRISYPTIPPKVEYLSTKRGKSL
                     ESILLELCNWGKQYMR"
     gene            36985..37365
                     /locus_tag="ACGI59_001198"
     CDS             36985..37365
                     /locus_tag="ACGI59_001198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis isomerase"
                     /protein_id="HFG9739001.1"
                     /translation="MTNADGLSESQMQKIARELNNSETAFIFNNLAKNHQENYDVHIR
                     FFTPTNEVPICGHATIAAHYVRAIENQFNNKTVFQKTGAGILPVDIIKKDNDYEIIMT
                     QGKIEVSPPLQQTYQKRLLNAFGI"
     gene            37425..37823
                     /locus_tag="ACGI59_001199"
     CDS             37425..37823
                     /locus_tag="ACGI59_001199"
                     /inference="COORDINATES: protein motif:HMM:TIGR00654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis isomerase"
                     /protein_id="HFG9739002.1"
                     /translation="MIGIKELSTLNSLSPNLYELSKISQEIKCNGYYIFTLHNKETLQ
                     VHGRMFAPAIGISEDPVTGNANGPLGAYLVHYKVIPTPNTEFIFEAVQGEAIKRKGKM
                     LVKVIILDNKPVEVKIIGKAVIAFSTNIEI"
BASE COUNT        12866 a         5945 c         4384 g        14720 t
ORIGIN      
        1 atttatctct atttttatat taaattactt gtgaaatatg tattctaaat tatatattat
       61 tagatatcat gcttaaaaat tctttggttg ctaaggataa agatatatct tttaaatggc
      121 aataacctat atttctctta ggtattggct cttttatatc taatttaaac aatttctcat
      181 tttccaaata atctatgcta aattcttcta taacacaaga cactccaaga tttatatacg
      241 caaattctaa aagtaattca tgagaaccca attctatatc tgggtttagt tttatacctt
      301 ttgataaaaa atattcattt acataaagtc ttgaatttgc tttattttct agcattatta
      361 aaggcaatgt gtttaattct tccaatgata ttttcctacc tttgtattct atgtaatcat
      421 ttgcacatac aaatatatca tgtatttcaa tacactcaac aatatttagt gtatcatctt
      481 ctattggcat gtttataatt gctatatcta tattcccatc tttgagtaaa gatataagtt
      541 ctctactcgt tctatttatt gtagatacat gtatatgtgg atacttctta tgaaaaattt
      601 ctaaatattt taacaagaaa aaacgtgcag ctgtatctcc tactcctatt ttcagagaac
      661 cgtattctaa ttttttaaat ttatttattc tatcttctcc tgatgatatt aactttaatg
      721 cagtagtgac atgttcactt agaattttcc cctctggagt taattttact cctttattgt
      781 ttctataaaa taataaagta tctaattgtt cttctaattg ctttattgac tggcttacag
      841 ctggctgtga cataaacaat tccttagcag caagagaaaa actcttatta tttacaactg
      901 cattaaaaac cttataaagt tcaagtttta tattcatata taattactcc ttatgtcaaa
      961 tattagtatt atttatttta cttataatta tatttgaatt ataatataca tggtactaat
     1021 atgacaatat taattcttta ggaggatttt agataatgga tagattagtt ggaactgttt
     1081 caagaggggt tagagcacct attataagac aaggagatga cttagttaaa atagttgttg
     1141 attctgtttt aaatgcgtca aaaagtgaaa actttgaagt tagggacaaa gatgtaattg
     1201 ccgttacaga agctgttgta gctagagcac agggaaatta tgctcacgtt gacaatattg
     1261 caaaagatgt aaaagataaa tttggagatg atactgttgg agttatattc ccaattttaa
     1321 gtagaaatcg ttttgctata tgtttaaaag gaattgcaaa aggatgtaga aaagtcgtat
     1381 taatgttaag ctacccttct gatgaagttg gaaatcactt aataagtatt gatgaacttg
     1441 atgataaggg aataaatcct tggtcagatg ttttaacaga agaaaaatac agagaactat
     1501 ttggatataa caagcataca tttactggtg tagattatgt tgattattat aagaatttaa
     1561 tcaaggattg tggtgcagag gtagaaatag tatttgcaaa caatccaaaa actattctta
     1621 actatacaac aagtatttta acttgtgata tacatacaag acaaagaaca aagagaattt
     1681 taagacaaaa tggagctaaa aaagtatatt cacttgatga tataatgaca gcaagtgtag
     1741 atggtagtgg ttacaatgac cagtatggac ttttaggttc aaacaaagct acagaagaaa
     1801 cagtaaaatt attccctata aattgtgatg aagttgtaaa caaaatccaa ggtaatataa
     1861 aagaaataac agggaaagat gtagaagtaa tggtttatgg agatggagct tttaaagacc
     1921 ctgtaggtaa aatatgggaa ttagctgacc cagtagtatc tcctgcttat acaaaagggc
     1981 tagagggaac tccaaacgaa gtaaaattaa aatacttagc agataatgat tttgctgact
     2041 tatctggtga tgagttaaaa gaagcaattt caaaatacat agttgaaaaa gataataaat
     2101 ctgatgattt aacaggaaac atggtttctc aaggtactac tccaagaaga ttaacagacc
     2161 ttataggttc tttagcagat ttaacatctg gaagtggaga taagggtact cctattattt
     2221 acatacaagg atatttcgat aactatacaa aatagtatgt tttaataaga tttaattttt
     2281 ttggattata tatattaaca tgaaaattta ttaaaacctc ttaatagata tgacttttca
     2341 agagttatat ctattaagag gtttatttaa tgttcacaga taagatatat ttaaataatt
     2401 taaacaagat tgtcagaaag ttatataatc ttaaaaattt atataactct aattcttatt
     2461 tatttctgaa ataatatcac tcttcaaaat ttgtcttact ggaataatta cagcaactat
     2521 actaatagtg atatttaaaa taatcatacc tatgtataga ggtgtattta ttttaagaat
     2581 tggatttata tataaatata cacgttttac cataaaataa atcataaatt gtccaataac
     2641 acttccaatt accattataa tacttgcata gaatccatat aaaaaacctt ctcttatcat
     2701 cataagtaaa aacttattat ccgtaatacc catagctctt aaaattccaa actcatgtct
     2761 tcttgaaaaa attaagtagc ttactgtatt gattatatgg aacaaactta ttgcaagaag
     2821 tacaaataca ataccatata taaataagag tttttgattt aagtaggtat tatttttttc
     2881 aatagcaatg gtataatctg ttacgataca tctttttaca tcacttacaa cactttttat
     2941 gttctcagac aattcctcac tgttactatc ttctgtcttt tttatactta ctgaattata
     3001 attttcgata tcaaagttat ttttcatttg cccattcgtc ataactatat ccataccata
     3061 atcaccaatg aaatatatgt tatttgccat cacacgtttt acagtagcag caactacaaa
     3121 ttcttttgtt gtataatcat tcttattttc ttctttaaat ttaacaattt catctgttgg
     3181 tttcattgtc tttggcactt taacttttat tgtatctcct ggatttatat caactgcatc
     3241 atacatccca gttccatcca taggtagtct aactataacc ttattatttt taaccatatc
     3301 atgggtatta atctccccat ctataatata agaatttaat ccttctaaca taccattgtt
     3361 ataaccataa atatttgttt ttaaaagata gccaccttca ttttgctttg tacaaatacc
     3421 atcaaaaaaa gtctttatcc tgtaatcttt ttcaagaggt ttaaagaaat ttttccaaaa
     3481 tagttgttct tctttaattt gcatagctcc aaataaatat cttactggat agacttttga
     3541 tattccctca acattgttta atcttgaaat tttatctttt ggtataccta catcaaaatc
     3601 tgttgtttgc atagttattt ggtagtcaga atttaaacca tcatcagctt tcattgtaag
     3661 ttcattatta tttttagtaa gctgtataga gtaactagaa cataaaaata taattccacc
     3721 aagtgataat gaaaacaaaa ttccaataaa cattcccttt ttttgagtca tgtatttatg
     3781 agataatact tttgtcatgt tagagtgttt tacactttct aatattctct ttttcttatt
     3841 agtaatctta aagtttttca tagattctat actagagtat ttattcaact gatgtacaac
     3901 acgtacagca attaaaatta acaacaataa gataaataaa aagcctaaat aaatagattt
     3961 ttcacttata aaaaatgctc ctggaacaat atcactactc aaaaatatat gtggaaattg
     4021 ttcatatatt gtagacgcca cttcaatacc taagaagcaa cctataggaa atccaacaat
     4081 aaaaagtaaa aataactcta aaaatagtag aaataaaagt tgtttattat tagcgcctaa
     4141 tacttctaaa accccatatt gtgatattct ctgaagtatt gaaacatgga aaatactata
     4201 tactgctgct gcaccaaaca ctccaacaag taatataatt gcatttgtat ttagttctaa
     4261 attttttaag aactcaaata tattatattt ttctatattt tttggtggtt ttactccaaa
     4321 ttcaactgaa agaggccagt ttatataagt tttttctttt gatatgttga attttttcat
     4381 aaatttataa ttttgctttt taatattttt attttcatca tattttacat aaactatata
     4441 agaatccttt tctttaggct gaaaataatc gtttacaaat ccctccagag cagtttctgg
     4501 gtttgaaaga cttgataaaa taccacataa cttatagatt ttaccatcta aattgatttc
     4561 tgttccaatt tctttgttta catttaaatt gtttagagaa tatgtgtcaa gtactatctc
     4621 tccatcttta ctaggaagag ttccttttaa taattttcta cctgtcatgt ctaaatatga
     4681 agaatcagca tttaaaaatt ctataataaa aggttcatct atctgatttt taggttttgt
     4741 aacgccaagt ctatctacag tgtattcctt tgatttttta ttttttttaa ttttattaag
     4801 ttgttcctta tctactttat agaaataatg ataatctcca ctatcttccc ttatcttttc
     4861 aattctactt ttgtctgcac tatgaactaa agaaccaatt cccgtcaata atgctactga
     4921 tagtactata cttaaaacta tagatataga ttgtttttta taatgcttta tatattgaat
     4981 tgaaaatttt actagagaca tcatagtact tcaccaccag tattcaccac aactccatct
     5041 tcaatatgga taattgagtc gcatacttgt gctatatttt cattgtgtgt taccataagt
     5101 atcgtttgat ggaattttgc actactttct tttaataaac ataccacttc cattgtagtt
     5161 ttactatcga gatttcctgt tggttcatct gctaatataa gcgcaggttt atttgccaaa
     5221 gctctagcaa ttgctaccct ctgttgttga ccaccagata gttcgcttgg aaattttaaa
     5281 cgcttatccc aaattcccaa tgtcttcatc agcatttcaa tatattcatg gtctatatgg
     5341 tttcctttat ctaatgtaac aggaagagct atattatcat aagtgtttaa tacgggcatt
     5401 aaactgtagt tttgaaatat aaatcctata tttcttcttc taaacactgt caaatctttt
     5461 tttcctattt tagatatgtt ctttccttta attattacat cacctgaggt tggattatct
     5521 aatccaccta taatatttaa caatgtgctt tttccagaac cagaagttcc aactatagca
     5581 gtaaatgtat ctcgttcaat ttctatactt actccattta aggcttttac tttagtctca
     5641 ccttttccat agtgttttac taaattattc gtcactaata tactcattta atttctcctt
     5701 tcaatacaga tatatgttct ataaaatatt gtaagtgacg ataattacac tcatcctaca
     5761 aaattagttt aaactcttac aattctgtaa gaagatacta aaacaacatc cacttcctag
     5821 tttagattct actataatgt ttccattttc tttttctaaa attaatcttg taagatatag
     5881 accaatccct gtgccatttt ggttttcaac atttttactt ctgtaaaatc ttttaaaaat
     5941 atcatttagt tctctactat caatacctat tccctcatct ttaatagtaa tcttcgtata
     6001 tagttccatt tttattacag aaatagtaat cgtgctatct gatggtgagt attttattgc
     6061 attttctaaa acatttacaa ttgcttctat ggtccacttt ttattatgaa ctaattttat
     6121 gtctgaaaat ggttcgagat ttatccttat atttttagct tctgctttat taaagatagt
     6181 actgatactt tgaataagag tatctttaat tagtagttct tctacttcaa attctataac
     6241 tccatcctct aatttagtca ttttaaataa ggattgtaac agccagtcaa tcttaaaagt
     6301 ttcatttttc atcttttgta aaaactcctt cttctgtaat gtggaaatat ttatattctc
     6361 cagaagttca caataaatgg ttatatttga aagtggagtt tttaattgat gtgacatatt
     6421 tgatattaaa cctttaatcg cttctttttc tgactttgac tgttctacct ctatttctat
     6481 catatcctta atttttttta cttggtgaga aatttttgac gctcttgtat cctttgtatc
     6541 tggataagta acttctttac cttccaatat ttgatttaac atcaaattta attcatcata
     6601 tctttttcga tttattttag tagatactat aaatataaca actgtacata ttataaaggc
     6661 taatataaac atattattct acctcattcc ataaataacc cattccataa gctgttttta
     6721 tatattttgg attagatggg tcatcctcta cttttactct aagtcttctt atatttacag
     6781 atacaatatt atcatcaaca taattttctt cgctgtccca aatatggtgt aaaatttgtt
     6841 cctttaataa tatttgacct ttattttcaa ttagataact tagaagctta tattctacag
     6901 agctaagttc caaacattct ttatttttat atacttttag taacttttgg tccaatttta
     6961 ttccattgga atatattact ttattattcg atttttttct tattgctact ttgactcttg
     7021 cctttaatac agataaagaa aatggttttg taatgtaatc atcaagccct atttctaatc
     7081 cttttacttc atcaatttct gagtctcttg cagtaagcat tataattggt atatctgact
     7141 tttcccttat tatttgacat acatcaaatc catctccatc tggaagattg cagtctaaga
     7201 ttataaaatc tatattttta ctattaaata tatttaatcc ttcttgtttt gtataagcct
     7261 taaatacatt aaatccatca ttctcaaatg cataaaaaag accttcattt aagtttatat
     7321 catcttcaat aattaataag ttcaatttta ctctccttta gattttttag ataaataatt
     7381 attttcttgc attatcatac taaaaatatt ttaattttat ttatttcact aaaaagattg
     7441 tatttaaatt tgttttcaaa ataaaaacat tataatcaag catatagtta tatatttccc
     7501 aatttaatta cgcttaaatt gaaaatataa ctttattctt atataattaa attatacaac
     7561 aataaagtta tattttttaa atatattttt actaggggga atagaattga aaagggttga
     7621 tgagagaaat actatgtttg caagagcaaa ttacaaaaaa gatagtactg cttataatga
     7681 ctattataag aaaaatcctg acaaaaaaga aattgatgat agtataagaa atagaccaaa
     7741 tttatgtagt gaaggaacaa tgacttacaa tgagttaaac tctccaatgg caagtagtgc
     7801 attcgatttt ttatctgata tcaaatcttt atgcgaagga aaagtttctg atactaaagt
     7861 agatgtagat gcaaagtcaa tgacaaaaaa aattaagggt cttgcaaagc agtatggagc
     7921 ttctgtagtt ggtataacta aattaaaaga ctatcatatt tatacaaata ggggtagaca
     7981 tgaagaaaat tacggtgaag aaattaatct aactcataag tatgcaattg tatttggatg
     8041 tgagatggat aaagaaatga taaatagagc tccaatgata tgtgaagtta tagaaacatc
     8101 aaagtgctat gtggatgcat ctatcgtcgg catgatttta agttactata taagaaattt
     8161 aggatatgat gctagaaatc atatggatgc taattattta gtaatgcctg tttttatagc
     8221 tcgtgatgct ggtttaggag atataggaag aaatgctata cttacaaata aagattatgg
     8281 ctctagactt agattaggtg ttgttactac agatattcct cttttagaag atgaatatgt
     8341 tgattttgga ctagaagatt tttgtaaggt ctgtaaaaaa tgctctttta actgcccttc
     8401 tcattcgctt tctaatgata ttaaaattgg tgatgatgga aaatataatt gggtaattga
     8461 acatgagaca tgctatatta aatggagata tttaggtaca gattgtggta tgtgtatatc
     8521 tgcttgccca tttagccaaa atctcgaaac tataaagaat actacttcat ttaagggtaa
     8581 taatgagctt attcaaaaag ctttagatga gtatacttca aaatttggta aaagaatatt
     8641 tatacctgga aatccatctt ggttgaaata attttattta ataaagaatc tgtactagaa
     8701 tttatcatag gacaggttct ttattaaata tataaatttt taaatacttt ttctcgttta
     8761 taacaattca gcaatgtttt tagtctataa ttgacattat aaaatattaa actaaaagga
     8821 gtaactgtat ggaaaaaata ttagtagtag aagatgattc tatattaaat aaaactctat
     8881 catataacct aattgaagat ggatatataa ttacttcaaa atttacagct aaatctgcac
     8941 taaagtctat ttttgaatgt gaatttgatt taataatttt ggatattaat ttaccagata
     9001 aaagtgggtt tgaattatgt aatgaaataa aggaaaatta caatatacca ataatttttt
     9061 taactgctaa tgatatggaa tgtgatatga ttaaaggcta tgaacttgga gctttggatt
     9121 atataacaaa accatttaat atcaatatat ttaaacaaaa aattaaagct tttttaaatc
     9181 atcttacaat aaaaacaaaa caagattact atagggatgg atatcttgaa attaactttt
     9241 ctgagttatc tgccaatata aatggaaatc aaattatatt tacaccatta gaatatcgta
     9301 ctttaaaact gcttactgaa aatcctaaaa gtattttaac aaggaaagta cttttagaaa
     9361 aactctggga tatagatgct aactttgtag atgaacatac tttaacatct gttataagta
     9421 ggattagaag caaaattgaa aaagacaatc ttaagtatat caaaacggta tatggtatgg
     9481 gatatatgtg gttaggtgaa agaaatgaat tttaaaaaat tttcttttag ttctatttat
     9541 ttaataattg ctgttggtat aatttttaca gctattttat ttagtatctc aatttatcta
     9601 ataactgatg atttaaaact aattgctcta ttattaatat atacaatagt actttgtgga
     9661 tttatagcat taataatatt tttacttcat aaaaaaatag ttatattttg ttctagaata
     9721 tgtcacactt tagatagtat gatgaatagt gaagataata ttgaaattga attagaagaa
     9781 gaaactattt tatcacgtat caatcacaga ttaattagaa tgtatgaagt tattcaagaa
     9841 aatcgcaata gtattgctat agagaaagct gatttacaag aactagtttc tgatatatct
     9901 catcaaatta aaactcctat tgcaaattta aaaatgatta atactactct actaaatcaa
     9961 acatttaaaa cagaaattca aaaggatttt ttaatggata tggaaaacca attagataaa
    10021 ttagattttt taatgcaatc tatggtaaaa acctccagac tagagacagg tataatcaca
    10081 ctatctaaaa agaaaaattc aatttatgaa acaattgcta cagcacttag tggaattttg
    10141 tttgatgctg aaaaaaagaa tattgaagtt acagttgatt gtgacccaaa tttatatata
    10201 attcatgaca agaaatggac ttctgaagct atttttaata tattagataa tgctgtaaaa
    10261 tacacaagtt ctaatggtaa gatacgtgta gctacagaat gttgggttat gtatacaaaa
    10321 atagatatta ttgataatgg aaaaggaata tcagaaagtc gccaagcaga aatctttaaa
    10381 agattttatc gtgaagaaga tgtccatgac atagaaggta ttggaatagg tctctatctt
    10441 tcaagaaaaa ttatatctct tcaaggtggc tatattaaag ttacatcaga tattggaaaa
    10501 gggtcgactt tttctatttt cttacctaat aaataaattt ttaaatattt catttgtaat
    10561 gattctgcaa cattttggtt ttatactgta tttacaagat gttaaggagg tattattatg
    10621 agtattttac aagtaattaa tttaaagaaa tattatggac aaagttcaaa cattacaaaa
    10681 gctcttgatg gtgtaactct atctattgaa aaaggcgaat ttatttctat tgtaggtaca
    10741 tctggtagtg gcaaatccac attattaaat atgatgggag gattagataa tcctacatct
    10801 ggaaaggtta ttataaaggg taaggaatta tctactatgt ctgatgaaca gcttacaatc
    10861 tttagacgca gaaatatagg ttttatattt caaaattata atcttgttcc tttacttaat
    10921 gtatatgaaa atattatctt accaatagga ttggatggag agaccataga taaggatttt
    10981 ataaatgaaa ttataaatat gctttctctt gaagaaaaat tatatgatat accaagtaat
    11041 ctttctggtg gtcaacagca acgtgttgct atagctagag cacttgtaac aaaaccagct
    11101 attatactag ctgatgaacc aacaggtaac cttgatagta gaactagttc agatgtactg
    11161 agtttactta aagtgacaag taaaaaattc aaccaaacaa ttgtaatgat tacacacaat
    11221 aatgaaattg cacaactaac tgatagaatt attcgtattg aagatggaaa aattgttaac
    11281 taggaggtgt agtatatgtt tagaaataat aatcgcacat ttataagaaa aatagcttta
    11341 aatgatttaa aaattaataa acttaaaaca tatctttcag gtatcattat aatgatttct
    11401 acatgtttat tattaacagt gaccttagtt tcatataatg cctctgtaga tttagttaat
    11461 gcatctccat atcatgctat atataaatct gtagatgaaa aagcaaaaaa tatactatat
    11521 aaagataaaa actttagtgg cgtaggaact tacaaattaa ttggaagtaa taaaaagaca
    11581 gattgtatta tgtcgattgt ttatgcagat gatacagcaa ttaaacttat gaattttcaa
    11641 ccattgaaag gcaaactacc tacaaataaa aatgaaattg ctatttcaga aaaatattta
    11701 caggaatttg gtttagataa agatattgga gatagcatta aattaaatta ttatgatgat
    11761 attacgaata aagaagttca atgtgatttt attattgttg gatttctaga aaattattat
    11821 caagataatg ccaagcaata ctatacagtc gtttcaaatg attattttaa gaatatagct
    11881 tctacacctt tacaaaataa aagtaatact tttgacgagt caagacctga tactgtagat
    11941 gtattagtta aactatcaga agaaaataca gagaaagatt cagcttcaat taagacacag
    12001 ttaaataaaa ttggtttatc tttaggtatt aagaaatata atatatactt aaatgataac
    12061 tatattgaat caaatttaat tgatggtgag cagattatta cagttttagt agttggaata
    12121 gttgttcttt tttctagcgt ttttgttata tatagtattt tttatatttc agttgttaat
    12181 tcagttcaga tgtatgctaa gttgaaatcg ttaggtatga cttcttttca gttaaaaaaa
    12241 attatttctc tacaaggaaa tattttgtca attattttta ttccattagg ggttatagct
    12301 tcttgtataa ttgcttacat aattcaacca ttagcatggc aaatgatagc tgatttattt
    12361 ataattttaa ttttatcact ggtaatgttt ttaactgtta gaatttcttt atttaaacca
    12421 gctagaatta tttctaaaat atctgctatt gaagccatgc aatatacaga aactaaattt
    12481 agaaaaaaag caaaaaattt tagttatata aatataaaaa atctagcatt aaagaatatc
    12541 gagtcaaata gaaagaaaaa tttgattgca ttaatatcgc taagcattag tggagtttta
    12601 tttatttcaa tcgctaatct tgctaattca atagatttca aaaaacaact atctcaacaa
    12661 tttgtctata acgaagatta tataattagc attaagagtg ataacttata tgagcatatt
    12721 acagaaatcc aacaaaataa tccactaaca gattctctta agaatgaaat taaatctatt
    12781 catggagtaa aaaaattaat tgaatccaaa tcaattaaaa gtaatattat agaaccttac
    12841 atcaaagata gagacgaaaa acaatttatt actctaatta aaggtattac gcctgaactt
    12901 tcaaaatcac ttgaagaata tattgtcagt ggaaaaatag actatacaca tttagattca
    12961 gatagtttaa ttataaataa atatagaaca aaatattatg gattaaattt aaaagttgga
    13021 gatacagtta catttaatat ctttagtgga aatgatatta ttaaaaagaa aatgaaaatt
    13081 ataggaatca ttgacaattc caatacagga ggtatgtttt ttgcttcaga caaaacttta
    13141 gaatctctaa caccatataa tacaaattta gatttttcta ttttaataga taaaaagcat
    13201 tctaaagaag ttgaagaaaa gttgttaagt ataattcaga aaaattcaaa tttaatgctt
    13261 tattcatatg aagatgatta tagaatgatt actagagcat ttcaatatat aattatagct
    13321 tcatacattt ttgttggttt aatttcttgc tttggtatac taaatatgac caatacccta
    13381 ataaacagcg ttttaattag aaaaaaagag tttgcattac ttcaagccgt tggaatgaca
    13441 agaaagcaac tgcgaaatat gctttataga gaaggtctaa acataagtat caaagctata
    13501 tgtacttcaa gtatactagg ttattttggt tcaaatctac tttgcacttt tataaaagat
    13561 gtaattagat tagactttat taattttaaa ttttctatat ttactatttt aattttttca
    13621 ttcgtattaa ttggtataca agttttagtg actgaattac ttgtaagaaa tatagaaaaa
    13681 aaatcactta cagaacgctt acgttctcag taagaatgta tataaaaaat agccattttt
    13741 tatacaagtt ttaacataat tttttaaaag ctaaaaggat agaaaatttt gactttacat
    13801 aattttctat ccctatactt gtttttattt tatacagata cacttactta tatctccgag
    13861 caacatcacc agagacataa acaagcatat tccttatttt ctaaatatgt caattgcatg
    13921 acttgtaacc cctactaaat cttcacgttc acaagtagca aggtgatatt ttaaatataa
    13981 tgcaaatgca tcatcatcca tagagtctac tgcttcacgc atatacaaag ccaaaccatc
    14041 tgatgcgaca taatgcagtc tagttacatt aaatgcagac atcaaatcat caatgttttc
    14101 tttacgtaca agctcaaaca aatcctttgg ttcagactta gcagcaaatg tctgtggatc
    14161 aatcaaacct ttttcaatat attcagatac attaatattt cctctatgga acccctcgtc
    14221 aataagacag ccatcagata taacatatgc agcaaaaacc acaccaccag gttttgttac
    14281 acgaattgcc tcatgtaaag cttgttgttt atcttctttg ttataaagat gatatagtgg
    14341 tcctaacagt aacgtaatat catatttatt atctgggaaa acagataaat ccatagcatt
    14401 accctgagta atgcttatat tttcatttga ttgagtattt ttatggaaaa cctctatatt
    14461 atgttcgact aattccactg cgtcaacatc atatccctga cgtgccagtg tgtgagaata
    14521 acgaccagtt gcagcgccaa tttcaagcac acaatcacct gattttatgt acttatcaat
    14581 gtaatgcata gtagtgagaa attccaccat tccatgtttt aatgcaagtc gactatcttc
    14641 atcataagta ttataaaagt caattaagta ttgttttgtt tccataattt cacctcttct
    14701 atattagtta ataaacatca aatagacaaa ttccaatctg atttttcttg tcttataaaa
    14761 gctgttaata tatttttcaa cattttttca gcactatatc tatctaatgt taattttttt
    14821 gttgctgtta agacagctat gccatgagta taaagaaata aatctaaaaa tattactttt
    14881 gcacattcca aatctattcc aatgctttcc gaaaaaagtc ttgccttttt ttcattgtca
    14941 atttctttat aaaaatcatt cgcctctttc atcatcaaat ctaaatcatt tataaataac
    15001 aacttaaata aatacgtttc ttcttgagca aattcaatgt atgaaagcgg aagcagtaaa
    15061 taagggctta cacttttaga attacgataa ttaacaacat actgattata atattcatat
    15121 gcaaaagcaa gaaattcatt ttttaattca ttcatgtttt cataacaagt aaaaattggt
    15181 cgagtggaac actgtaattt acttgcaata ctcctcgcat ttacagcttc aaatcccact
    15241 tctcttgtaa tatccaagac tgtattcaaa atcatttctt ttgttatctt tactttaggt
    15301 ggcatatatg ttcacctctc cattaaattt ttataaaaca catgtgttat ataactatag
    15361 taacatatca agaaattatg tcaagaaaaa gctttgcatt tttttgcaat tcctaaaact
    15421 tataaataaa acatgagttg caattaatct attaatagat aacagcaact cagattttcc
    15481 tttaaatact cttctttttg tctaatattt catatacaca atcttctatt gaattaatat
    15541 ctttttcttt tcttttttta gaatcaatta aaatgaaata cagtataaaa cctacagcaa
    15601 gtccccatgt tccgccttta gcagcaatta cacctcccat aagtccaatc aatcccttct
    15661 caacacctgt attacatagc tccattgata gctttgtaca taaatatcct tgaactaaaa
    15721 gtgttactga caatgctaat ggtataagag gtgtagcaat ctgcgcaatt ggataaaaac
    15781 agataaaaat tgctgaaacc catactaatg tacccatgcc actaaaaaca gactgcatgg
    15841 cttcctttcc aaccttgtac ctttgagcaa cacttcctgt aagtgtcgca gctaatggac
    15901 cgcacattgg tatatatgga gaaaagattg ccataaaaat atttcttatc ccacttataa
    15961 cattagacct atttgaattg aaatctatat attcatcttt tctattttca ctttcagaaa
    16021 gtactaagtt ttcacctgtt ataaaatcac caaaggcaat tatgtatatc ataaaagcca
    16081 ttggtatccc ttgtataaat aacattacac ttggaaaacc tattccaaaa ggagatactg
    16141 tatttatcag atttttaaaa tctactatgt aaactaaatt gtcaaaacta aatctaggaa
    16201 tttctatttc tttactaaat atacctacta ttaaactcac taaaatagct gatactacac
    16261 catattctcc taaatgaaat aaaaatttat tcttaacttt tagagtatca aatctttcag
    16321 aaaacattac aaaacaagca accaaaattc ctactgttat agatatagga taagaatcaa
    16381 atctcccttt aggattaaac tctgatatta tagtcgatat accgacaccc attagtattc
    16441 cacctttaat agaatttggt atcttactaa ctaactttcc agctacacca gttataccca
    16501 ttaaaataaa tattattcct aaatctattt gtaaagctgt taatgcttgt attctttcaa
    16561 tgcccatgct gaactttaac aaatatgcag ttgtaagagt tatagaagct gtaatccagc
    16621 caggtacaac tgggtctccc ataaaaacat gcaaacagta taatattgag ttaatgatag
    16681 ccatactcca tgccacttca tagggtaatc ccaaaatttg tgtactaagg ccaattgtgc
    16741 ctaaacttgt catacttgat attatacctt gaatagcttc tggtttctca aatctgtaat
    16801 gtataaatgg aattcttatc ttaaacattc caaattttat atgaggttgt tcccttctag
    16861 cgagtctttc cataacttca ctccaccttt caataactct tataagttta caactttata
    16921 attgtttata aattatcaat gtcattaaaa ttagtatata acatgcaaat tatataaaca
    16981 ataaattaaa tctataaaat tgatagattt tgtgcatata tagaaagacc gcttcatttt
    17041 tagttactta aatcacttaa tagtagttat ttaaatcact tgataaaggt tttttataaa
    17101 attcaatagt tctattccat gattcaaact gtccttttga atttccttct acagttccac
    17161 ctgaagaaaa aatccctcca ctcatactca tacataaagt ctcctttaga ggcataatat
    17221 atggaattgg taacggatga cccatattat tgtatgttaa taattctacg ttatatggat
    17281 aattattttc ttcaaagatt tctaatgcct ttaaacaagc ttcataacta ttccatatgt
    17341 tatcttcttt tccagcaatc ataagtatat ctgctttgga attttctatt tttattctag
    17401 cctcttttct attctctgct cttttaagac ttttcttata agtactatta aatccaaatg
    17461 gaataccttt tgatatattt tttttctgtt cttcaaagaa tatatcattg tctactttga
    17521 tatatggaaa tggctctcct ttatatgacc atgatgaagt atcatttcca ctcatcaatc
    17581 cattaagagc ttggaaacaa taaatgtgag gttgtgatac tacaaccttt ttaatttgtt
    17641 catatcttga agcaagtaaa aaggccaatt ctccaccctt tgatgtccca tgtacaaata
    17701 tttctttagt actggtaatt tcattttcac taatccactc aaatactttt tcaaaatatt
    17761 ctaatggaac ctcttctaat ttatctggta acccttctaa agcaaagtat gcaactgtta
    17821 gtacattgaa tcctctagat gcaagtggag ctgctaaaag gtctagagcg tctaaatttc
    17881 catcagaacc acctagcatt aaaattgttt tattattaga attttgttta taaaataatt
    17941 ttcctataaa ctcatcagat atatattttg tggtaatatc ttctgattta aatattctct
    18001 ttaattcaac ttcttttctt tcaatagaag tttcaaatat catattcata attattggtt
    18061 tatcaataga tatattttct gcaaaatttt taccttcagt tttagatttt tccagcgaat
    18121 aaattaaccc catacaatta tcagccttat aagtaccttc tataggttca actaaatcta
    18181 aatctacttc tcctttttca tttgaacaaa acactccata agatgaaaat tcttctccct
    18241 tgcaccatgg aaattccatt ttcacactta ttttaagttt agtatttgga attaagtccg
    18301 aaaccataat cttaagtctt tcatccacaa atgattctac attgtataca tttactttca
    18361 taatctattt ctccttataa taattattta tgttttatat aaaacagctt tatctaatac
    18421 aacataaata aatttttaaa ttaagacacc atattgacta tttcatttta tcaacatatt
    18481 ctatttttgt caacatattg tcttttttag caatttaaaa ttatgtagtc tatatgaaat
    18541 ccagttgatt tttggctaat taaagatagg aatgctatta ggtttagaat atatatattc
    18601 aaaataaaaa accactcatt ttaagaatgg tttttaatat aaactatttg ttattaataa
    18661 ggatattttt ttatttaaat ttatataatc gaacatctaa tatttaatgt ttaataaaat
    18721 tatgcatttg caaaagtata tatggcatta actcttgcta catataaatt ctcatcacta
    18781 catccaatat atctagcata tttttcaatc ttttcttttg cattttcaac atcattttca
    18841 tcgctctcca ttaactcatt tatataaata tccattaatg acgctttata ttctcttata
    18901 tcatagccta aaaattcagt caatctctca atttccttac taaaatctcc atcttctttt
    18961 accaaaaatt cgtatacatc ttccctatat ggatttaaag ttattatttg ttttactact
    19021 ttgcattcgt cagccttccc aataaaacca tctttataat tattaaataa ggcattagat
    19081 ttttttatag attcttcatc aatataagaa tgtaccgcag ttatacgatt ttctataagg
    19141 gcatctacaa cagcataatg aattttaaaa caatcattgt ataaataatt gctcattttc
    19201 tttattgtcg attctatttc taagaagctc gcgtggccat tttttttatt tttatttggt
    19261 ataataaaag ttttattcaa gttattaaaa agtctcttaa aatctaaata tttttcacaa
    19321 taaacacttt taaaggtatt gatatctata gttataattc caaaacttac tatagtctca
    19381 attcctttct taaaagactc ttctatatat ttttctccta aacttaaacc tctatcagat
    19441 atttgcttta gattttgaaa actatttaaa caatctctct caaagttttt agatatttcc
    19501 aaagcatata atttaaagat tttccttatc ttattataat tattttttgt atcatcaaat
    19561 ctgataatag ctccatttac atcaaaagta atcattgagt tttaacctcc ccattagttt
    19621 catcaataaa ataaatttaa tataaacttt ataatcataa tattaaactt atttatacta
    19681 taattattaa tttgatgata aaagctattt tccttctaaa taaaaaattt gtctaaagtt
    19741 tctaaaaaat ttagacaaat cttaggttat tactctttaa attattttta aattattttt
    19801 aaattattcc aaatacgcca aataatattg gtataaatat agctggtaac aaattggcta
    19861 cttttatatt cgttaattta agtacattta gaccaagtcc tataattaat aaacttccag
    19921 ctgctgtcat ttctgtgact acaggatcac ttaaaaatcc atttaaaaat gatgctccca
    19981 cacatataat accttcatac aaaaatacag atattgcaga aaacattaca cctataccta
    20041 aagaagcagt aaatataatt gaagctattc catctataac agattttaca aataaagtat
    20101 catgacttcc tgtaagacca ctttctaatg aaccaactac tgccatggct cctatacaga
    20161 ataataaact agatgatata aaaccctctg ctatagaatc ttttttattt cctttagaaa
    20221 atttactttg taaaaaagca cccaacttat ttacccattt gtcaatatct attaattctc
    20281 ctatcagagc acctacagct acggatatta ttgtcactaa tgtgtttttt ccttctagtg
    20341 ctcctgatat accaatgtac aatacacata aggcaattcc attcattata gtatcgctta
    20401 ctctttgagg taatcctcct tttattataa gacctactat acatccacct gcaattgcta
    20461 gactatttgc tattactcca aacatttttt ctcccctcga ttagctaatt gaaatttatc
    20521 ctattattaa aacctaaaca tctatacttt gtattggata ttttaaatat tccaaaaatt
    20581 cttcaaaacc ttttctagat atatttacag ttttagtatt tatgttagga tgaaaattta
    20641 agtaatcttc tttaattaca tctttatcta tgtaaagctc aacttttctt tctgtatcat
    20701 ttattaatga aaaaggattt acacttccag gaagaagttt aagtacattg tatagtcttt
    20761 caggtgaagc aaacgataat ctagaacttc ctatcttatc tttaagtgag tttaaatcaa
    20821 cttgtttttc tcctttaact acaataagat aatacttatt tcctttggca tttcttaaaa
    20881 ataaattttt acattgacac ccctttgtta ttccatctaa aatgactaat tgctctgctg
    20941 tatatacagc ttcatgctct actacatcat attcaacatt taatttatcc aaaatatcat
    21001 atattttagt ttcattcatg ctagattcat tttcatcttg ttctttttta ttatttaaat
    21061 cactcatact tttctcctta aacacttcat cataatatca ttgtgaggta ttattctact
    21121 atagctattg ctcttactgg agctccatca gcatcattat acttaaatgg tgctgctata
    21181 aatagaaatt tttctggaac attttctaaa tttgtaagat tttctatgat tatttttcct
    21241 ttttcaaata aaatatgatg cacttcaaat actgatgtgt catatctatc cactgatagc
    21301 atatctattc ctatgccttt tatattagta ttagctatat agtttgcagc ttcttttgtt
    21361 aatgtaggat aacctacata gtactgcttt ttatcccaaa attttgacca acctgattta
    21421 aatattataa aatcactatt ttttatttca tcttcatatt tttttagata ttctaattct
    21481 atattctcat ttcccttttc aagttccaat acataagctt tcccaacaaa attttctata
    21541 tccaacttat ctagggtctc acctttagta tacatatgtc ttggtgaatc catatgagtt
    21601 ccattgtgag aaaatacact aattaatgtt tcttgataac cattttcttc aataatagct
    21661 actttcttga tatctggtct attaggctct gcatatacag gcatgtcatt atgtgtcaca
    21721 tgagttaaat caaaaacctt catctttata taccccctat gtttttattt tctattatat
    21781 acctaaattc attttatttc tatcagattt tattccatta tataccatac tactatatat
    21841 tttttataaa cctcaaggga tttaccgaaa aatcttggaa agaattatca gtaaatataa
    21901 catcttcacc caaaaaacgt ttataccttc cttcttcttt ctttaaagta attctctgtt
    21961 tttgtgaatg attattaaaa accattatct taagaggagg tttttttcta ttagtctgca
    22021 ctctttttaa catgtatttt atgtgcatat cagcttcagg aaatgaatat ccacaaaata
    22081 ttaaaaggtc agtatctctt aaagtttttt ctgtttcatt ccaaacatca cttaaaaata
    22141 tgtttgacat attcttaaaa tatgttggtg gcactataat aggttctgtt aactcaccac
    22201 actctaaaca ttttgtctct gaactatttt caataagctt catgacacca ccttcatgtg
    22261 gtgtaagagt tacactatta caaatggggc aataaagcca attcaatgac ccatgtatct
    22321 tatataattt gattaatggt tcttttggtt ttctccaatt taactcctct ttaaaatttg
    22381 taaactccac accataatca agcataattg gcaaatcatt cttatctctt aatccagtaa
    22441 ctgtattgtc tatatgtata tcataatttg cactaataaa agtagtatca agaagcaaat
    22501 cattttccaa aagattttca actaaaagtt tatgatattt attatctgtt ctaggtgtat
    22561 tatgaattgc ttcagccatt agcagtatta aatattgtct taaaaaacgt atactatcac
    22621 ttctgttatt tagattttct agtccaaaat ttctaaatgc ttcttttctt tgttctgcta
    22681 agtctaaaag acctaaaact tcttcaaagg taggaaaatt tgcattatct atattatctt
    22741 ttactatatc aatattgaac atctgtttaa agaaaatata caattctcta ttcattttgg
    22801 tattataatt tgtgggattt agttttttaa aatattcgct aaaaagttca ttttgaatag
    22861 gtagattctc tgctgctgaa gctcctgctc ctaaaaatat tgctattttc atatagttcc
    22921 tcctaatata aatattactc ctatttttat atccattaaa tatccatatc atttatttta
    22981 tgatacaaca ataaagctgt cttttactag aaaaaatcct attacaagac agccttatat
    23041 tatattaaat ctttaataat tcgtaaatag aaagagcata tatttgagta gcttttataa
    23101 tatcctctac tattaagtat tcattagctt ggtgttctgt ttctggtttt cctggaaaaa
    23161 tagctccaaa tgcaacacaa ttatctaaag ctcttgcgta tgttgctcct ccagatgaaa
    23221 gtggtgttcc atcaagacca gtttcttctt catatacttt tctaagtgtc ttaacaagaa
    23281 gagtatcttc tggaacatag attgaatcca agaaatcata ttcttcataa tttaaattgt
    23341 atttatctgt cattttcttt aactctttta caaaatcatc ctttttgtat gttactggaa
    23401 tccttacatc tattcctgca aattcttttt cgttatcaat agttactcta cctatattta
    23461 ctgttagttt acctgataca tcatccttgc agttaggaat tatattgttt ccattagcat
    23521 cttcaccaat aacttctgct aaaaacttaa ttatattaga atctatacct atattattaa
    23581 gagctataca aagtctagcc actgcattta taccagtatc acttgctgct gaatggacac
    23641 tctttccaat tatacagatt ttattacctt caacagtata ctcaaaattt aatttattaa
    23701 gttctttttt taatttatca gagtattttc caatatagat tgctttacct ggaactgcat
    23761 ttaaagcctt tccaactgac aactctatat cacttttgct atcacaagtc aaatgaactt
    23821 gtaaaagacc tttctctgca tttgttattg ggaatctaga atctggagta aatccatagt
    23881 ttggtatttc ttcattattt tctttatatt tatttataca tctccaaaga ttttcttcat
    23941 cagccccaaa aatgaatctt atcctcttat taaagtctac atttaaatcc ataagagctt
    24001 tcactgcata aattgctgat attgtaggtc ctttatcatc ttgtgttcct cttccataca
    24061 atttaccatc ttctaaaaca gcttcaaatg gagggtaatt ccagctttct aaatcacctt
    24121 ctggaaccac atcaacgtga ccaagtatac ctatcatttt ttcaccttgt cctatttctg
    24181 catatccata atatccatct ttgtatactg tcttaaatcc taaagattta cataaatcta
    24241 atatacctct taaagcttta tctacatttt caccaaatgg gcaattttca gttgcttcag
    24301 atataacact aggtattttt acactttctt gtattgaaga aatcatttca tcttgtagag
    24361 aatttacttt ttctttaatt tgctctttca tattatcacc cttcaatttt catcataata
    24421 tactaataaa tattttgacc ttttaaaaaa aaaatatgct tattttttca gtttttttat
    24481 aaattcaatc gcttttaaag tccccatttt tgaatggtca aatgtaagtc caccttggaa
    24541 ataaacatta taaggaggtc ttattggagc atctgcactt aattctatag aagaaccttg
    24601 tataaatgct cctgctgcca ttatgacttc actttcgtat ccaggcatgt cccaaggaac
    24661 aggttttaca taagaatcga ctggtgcagc ttcttgtata ccttcacaaa aacttattac
    24721 ttcatcagca ttattaagtc taatacattg aataatatca cttcttaagt catcatattt
    24781 tggtaataca tcatatccta atttttcaaa agctcttgag caaaagatag ctcccattac
    24841 agcctgagat actatataag gcgccaagaa aaaaccctga agtacatttc tagttgttcc
    24901 aaaagtaaga ccacattctt ttcctattcc aggagaagtc attctataag atataagctc
    24961 aatcaaatct tttctaccag ctatatatcc acctgtcaat gcaagcccac caccaggatt
    25021 ttttattaaa gagccagcca taacatctgc tccaacatca gtaggctctt tggtatctaa
    25081 aaactcacca taacaattat caaccattac tatagcttct ggtttaacag actttattac
    25141 ttctatagct tcttttatat cactaattga aagtgatttt ctccaagaat atccttttga
    25201 cctttgtatc ataacgagtt tagttctatc atttattttg tttttaattc cttctaaatc
    25261 taagtttcca tcctctaaaa aatctacatc atcatatgta acaccatact ctttcaatga
    25321 acctttttct tctcttatgc ctataactcc ctctaaagta tcatatggtc taccagtaac
    25381 agataaaatt tcatctcctg gtctaactat accttgtata cataaagtaa gtgcatgtgt
    25441 accatttaca attattggtc ttacaagagc atcttctgta ttaaatactt tagaataaat
    25501 ttcctctatt ttttcacgac caatatcatt atatccatat cctgtagtcc agttaaagtg
    25561 catatcactt agtttagatt cttgcatagc ttttaaaact ttatattgat tatattctct
    25621 tatttctttt atttcttcaa acttatcttt tatttcttcc attatttctt gcgatagttt
    25681 aaaagtatca tcatctattc catagtagtc ttttaataat tcttttgtct cattaagcat
    25741 tttatcacct gtctctttat tattttattt aaacatgtaa aggagactat tttactaaaa
    25801 caaataaatt atagtttata agttagtata agtctcctct caatcatctt aaattaaatt
    25861 aattatctat ctgttgttat tattattgtt gttttggttt tgatttgtta gattaatata
    25921 ctttccaggt tgtatagttg atacagcatg tttataaatc atgttttgtt gtctattatc
    25981 cccttctatt aatattatat aactatcaaa cccctttaca agccctttaa cttgcactcc
    26041 attaactaaa tatatagtaa caggtatcct ttctttcctt gcattattta aaaacaaatc
    26101 ttgtaaattt aaaactgtat ttttcattag atttgtaccc tcccaagatt aaattgaact
    26161 tctaaaatat tcctacctta tattctatat aagttatgac aaattttcaa tattaagttg
    26221 tatacttaaa aatttatata ttattttata gaatctttaa tatataagat tatttcattt
    26281 tttaattcgt ctatattttc atattggtct aaatcaaacc attttgctgt tttatatctt
    26341 ttaaaccatg ttatctgacg ttttgcatac cttctagaat ctcttttaat tatttcaata
    26401 gcttcttcgt atgtatattc tccatccaga tactttaata tctctttata acctatacct
    26461 tgcattgata tcatatcttt tttaaatccc atacttaaaa gtttttttac ctcttcaacc
    26521 aatccatttt taagcataat atcaactctc atatttattc tttgatacaa atgttctcta
    26581 tctctattta gaactattat tattggttga tattcttcat taaattttaa atcacttgaa
    26641 aaatcattca ttttcttacc acttagacaa acttcaagag ctcttatgac ccttttagta
    26701 ttgtttttat ggattctatt agcagattct tcatcaagtt cttttaactt attatgcatg
    26761 taatctattc ccttttcagc aagttgtttt tgaagttctt ctcttaattc attattggca
    26821 tcagattgtg caaaatccat attatatatt aaagagttta aataaagtcc tgtgcctcca
    26881 gttactaaaa cacatttttc ctttttattt atttcatgta tatatttttt tgctctttgt
    26941 tgaaactctg aaactgaaaa tggatagtca ggcttaactt catctataag gtaatgcttc
    27001 actccttgca tctcttcctc tgtaactttt gcacttccaa tatccatata ttcatatatt
    27061 tgcatagaat cagctgaaat tatctctgca ttcatatctt ttgctaattt tattgacaaa
    27121 tctgtttttc caacagcagt tggacctgta agtattataa gtggtatttt tttcataaca
    27181 ttcctttcca tatattttaa atatccaaat attttacata attcttttaa acattttttc
    27241 tatttctgtc ttagatattt cgaccattat aggtcttcca tgaggacatg taaatggatt
    27301 ctcacacttt tctagctgtt ccaataaact ttttatttca atatcgtata ttttatcatt
    27361 tgcttttata gcagacctac atgccataga tgcaaatctc tctcctttta aatcataatt
    27421 acttgtaatt tcttctatat tatcaattat ttgaagtata aatttctcgg tttcaggtac
    27481 tccaaatata gtaggaacac atcttaccat tatatgatta ttgccaaaaa tttctagctc
    27541 aaaaccaaat ttcataaata actccaagtt attttcaatt tgaagcatat ctacatttga
    27601 tacttcaata accactgggt ctaaaagtat ttgcatattt atatcttgtc tatagaactt
    27661 ctccatatat ctttcatata gtattctttc atgtgcagca tgttggtcca acaaatacat
    27721 agaatcgtct ttgctcaaaa ttatataagt attaaataca actccaatta cagaatatcc
    27781 gtataaagaa aacttgcgtt ttgaactaca caagtattct tcttcactgt ccttaatact
    27841 atctcctaag taataatttc cttcattttt aattccatca acttgaaact cttcttgtat
    27901 attatcattt gcactatctt cacttagata attaatgttt tttacatcat cttctacact
    27961 tgcatttagg acttcactta ctgattgaaa ttcatttatt ggtttttcac ttgataaagt
    28021 aacaacttca atgacttcat ccttaccttt agtaatatta ctatttttag gagtagattc
    28081 taataaatca ttatttctta atttataatc tgtacttttt tctctactgt taatagcgat
    28141 tctaggttgt gtttttttat cactataagt agcatatttt ccaataagat ttgagtgtat
    28201 taatttaacc tttaaaaaat ctctaagttc tatatacact tcttgttctt tttcaaattt
    28261 aatttcaagt ttattggggt gtatatttac atctatacat gatgggtcta cttctatatt
    28321 taagaagcac actgcatgtt tacctattgg aattatagac ttataacttt ctgttattgc
    28381 atctatgatt attttacttt ttacaaatct tttatttata tagatatgtt gtaagttttt
    28441 atttgaacgg tatatattgt tattacctat atatccattc attttaaagt gattgcactt
    28501 aaactctaca tctattatat tttccgttat ttcttttcca tatatacttc taatagtgtt
    28561 tacaagcttt ccatcccctg gagtatttag catctgttta ttgttatttg tatatttaaa
    28621 ttgaatgttt ggattaccta ttgcaagttt atttatcaaa tcacttatat ttatagtttc
    28681 tgcatgagta gattttaaaa atttctgtct tgctggtgta ttaaagaaaa tatcttttat
    28741 aatgatggta gttccattag tagaacctat tggttctttc gatattattt ttccaccttc
    28801 aacatatatt ttagttccta ttatttcatc tttagtttta gttgtcattt ctaattttga
    28861 tacagctgaa atagatgcca atgcttctcc tctaaaacca agagagtata aatcatacaa
    28921 atcatcaatt tttttaattt tactagtagc atgtctcaaa aatgattttt caacttcact
    28981 tgaaggtatt cctattccat tatctgtaat cttaattaga ctttttcctc catcaattat
    29041 atctatggaa attttatttg cacctgcatc aatggagttt tcaataagtt cttttactac
    29101 agaagaaggt ctttctacaa cttcacctgc tgctatctta tttatagtca aatcatctag
    29161 gatgttaatt atatttttca tttttacacc tctaagattt tatttctttt gcttttcttt
    29221 gaagattata aagagagttt atagcatcta atggagtcat atttaaaata tccagattta
    29281 aaatttcttc tgatagtata tctctattta cactatcaaa agagatttga gttaaggcta
    29341 cttcttttac agaatccact tgattaattt tctctttatc atcattttgc tttattaaag
    29401 caacttcatc atttaatgct ttaaatttag aatttaattt atcataatcc ttttttatat
    29461 gtttgtagtc ttttactata agttcatgtt ctatttttag agattcatat ttattcttaa
    29521 aattactttg atttacctta actaattctt cgtccaaatt actattaatt atattattgt
    29581 tttctttatc tccatttatt gcaacactgt tgtaaacatg atttttttcc aagtctttaa
    29641 gtatatattt tgctctttct atgacttcgt ctggtaattt agctaattta gcaacatata
    29701 ttccataact cttgtctgct ccctgaggaa ttatttttct taaaaatatt attccttccc
    29761 catcttcttt gactgctatt gaataatttt taacctcttt aaactcttct tctaaatcag
    29821 taagttcatg ataatgtgtt gcaaataatg tcttacatct aatatttttt tgaatatatt
    29881 ccactattga ccatgctaaa cttataccat cataggtact agttccacga cctatttcat
    29941 ctaatataac taaacttctc tctgttgcat tttttaatat aagggatact tcgttcatct
    30001 ctaccataaa agtagattga ccttgagata aatcgtcact agctccaact cttgtaaata
    30061 ttctatctaa tattggaata tcagcagact ctgctggtac aaatgagcct atatgtgcca
    30121 taagagctat tatagcagtt tgtctcatat atgttgattt acctgacata ttaggaccag
    30181 ttattatgtt aataatgttt tctccacgat ttaaataagt atcatttgga acgaaatttt
    30241 cctctcctac tatattttcg acaacaggat gtctaccatt tcttatatca agtttattat
    30301 tttcatttat tgctggtttt acatagttat ttatatgtgc aactgttgca agggacacaa
    30361 aaacatctat attagctata gtcttagcca ctttttgtat tctatctatg tttttatata
    30421 ttgtatctct tatctctaca aatatctcgt attctaaaga tttaatcttt tcctctgcat
    30481 gtagtatctt ttcttctatt tcctttaatt ctggagttat atatctttct gcattactaa
    30541 gtgtttgctt tcttatatat gtctcatcta gctttgcctg tttaaagtta gcttttgtta
    30601 tttctatata atatccaaaa actttattaa agcctatttt taatgattta actcctgttt
    30661 tttctctttc cctgttttct atttccttta caagaaaggc tccattctta gaaatctctc
    30721 ttagttcctt aagttcatca ctaaaatctg atttaattat gtttccatct tttatggtta
    30781 tcgtaggttc ttctagaata gcttcatcta ttaaattgta tatatcatct aatttatcca
    30841 tttctgaaac atactctttt aggatttttg catttgataa atttatagta tcttttaaat
    30901 taggtaactt ttctatcgaa ttttttaagt gaattaactc ttttggtgtt actctctcaa
    30961 atgctatctt tccacatatt ctttctatat cgtatatatt tttaagtata tcatttaggt
    31021 cttctcttaa tataaagtca tcttttattt cttctattac atctaatcta ttttcaattt
    31081 tagatttatt tatcaaaggt tcttctacat attttcttaa aagtctacct cccattgctg
    31141 tagaagtttt atcaagtacg tgtaataatg aacctttttt cttattccct cttatagttt
    31201 gagtaagttc taaattagtt ctagtaaaca tatctagaac catatattct gatgaattgt
    31261 atatatttat attattaatg ttagatgtta tttgtttttg agtattgtat atataattca
    31321 gaagaattga taagcttgac tttattagac ctttatcatc aaactttagt ttttgtagat
    31381 aaacatctgg aaaatattct tttaatatat ttatatccaa atagttatca ctaaaacttt
    31441 catttatgta tatattactt acagtagcta tatctctaag attttctata aagtctaaat
    31501 catttataat tatttcagtt ggatggattt ttgcaatttc ttcaattacc ttatcttcat
    31561 ttaaacatgt tgcattagtt tctcctgtac ttatatcaac ataagttaga cctatattag
    31621 taccatcctt atacaaagat aataaataat tattcttttt attttcaagt aaatttccat
    31681 ctaaaactgt accaggagtg atgactctta taacctctct tctaacaatt ccttttgctg
    31741 tagatgggtc ttccatttgt tcacctattg ctactttata tccattttca actaattttg
    31801 atatatatga attggctgag tggaaaggaa caccacacat aggagcacgt tcttctaaac
    31861 cacaagcttt tcctgtcaat gctatttcaa gagcttttga agctactaat gcatcttcaa
    31921 aaaacatttc ataaaaatct ccaagtctaa aaaacagtat acagtcttta tatctatttt
    31981 ttacttcaag atactgtttc atcataggag ttaatttatt catatctatt tgcattatca
    32041 aacctccttc ttttctaatt aaatattata tcatagtaga tgattattat taacacaata
    32101 tataaaattt tgacatataa ctaaaaacaa tccaaaatgt atactcacat tttggattgc
    32161 taagtttaat gcttattaaa taaaaatatt ttttttacaa taaagatatt atttcttttt
    32221 taagtttaat gatattttca aggttatctt catttaactt tctactatct ataacaaatt
    32281 ctttctttac tattgatggt ttttttgaaa taacataaat tctgtttgat agcattatag
    32341 cttcgtcaat atcatgagtt ataagaagta ttgttgaatt tacttttttc ttaacatcta
    32401 aaagccattt ttgcatggat gttttagtta atgaatcaag tgccccaaaa ggctcatcta
    32461 aaagcattat gtcattagaa tttataaaag ttctcaagaa gttagctctc tgtctcattc
    32521 ctccagaaag ttctgaagga tatttatctt catatccgct taatccaaac acctcaaaat
    32581 aaggttttac cattgaccta gctttactct ttttttcttt ttttaaaatt agtggtagtg
    32641 acacattatc tattatagtt ttatatggca agagtaaatc tttttgatac atataactat
    32701 acttaccatt tatattcact ttgccactat cataatctgt aagtttagtt ataatattaa
    32761 aaatagtact ttttccacat ccacttggcc ctaatataga aacaaactca ccttcataaa
    32821 catcaatact tatatctttt aaaacttcaa tatctttata agtttttgat acattttcta
    32881 tagatacttt tactttttta ttgtggtaaa aattcatttg tgtaagcatc acttgccttc
    32941 atatccttat ctattaattt atattctttt aagaatgatg tatagttgtt ccatacacta
    33001 tctttcattt ctccccatcg tggtgcatca tctttgtact tgcttgctaa atattcttga
    33061 ctctttaaag ctagtttttc atctacttca ggtgcatgtt ttactaaaat ttttgctgat
    33121 tcttctggat tttttattgc atattcataa ccttttgttg tagcttttaa gaatttttta
    33181 gcaagctctg gattatcttt aagcacagtt tcattagaaa ttataagtgg tgtataataa
    33241 tccaatctct catctaaatc ttttactggt ataaagttta agtcatatcc aattaaatca
    33301 gctttaactg catcccaacc ttcaaatatc catgcaaaat ctacagtttt catagctgca
    33361 aagaaatcat cttgaccagt attaactatc tttaacttat taaaatcagc tccatttttc
    33421 ttcataactg ctttaaaaac agcttcttct gatggagagc cccaaccacc ataagttttt
    33481 ccttcaaaat ctttagctgt tgttatattc ttttcttttg gtgatgcaaa cccagaagta
    33541 ttatgttgta taacagttgc tacagctttt attggaagtg gatcttcact agtctttgca
    33601 taagtaactt gttcttgata gcttatacca aagtcagctt ttcctgttgc tactaaagtt
    33661 tctgctccac tttcaggtgg ttgtactatt tcaacatcca aaccttcttc tttatagtaa
    33721 cccttatcaa gtgctacaaa taatccagta tgatttgtat ttggtgtcca atcaagcacc
    33781 atagttactt ttttagaaga agtatcttta tcattgtttt tgtctttatt gttatttgaa
    33841 gaacatccag ttattgttgc caatgtaaaa acacttacta aacctaatat tcctaacttt
    33901 ttaaaattca taattgcctc cctcaaatac ctttaatcta ttaatctatg tcttataaca
    33961 acttttttta cagcctgtac aattcctaca aacattaaac taaatataac tactaatata
    34021 gtagatgcaa atactttatc aagtgcatat gcactttttg acctaagcat ataaacacca
    34081 agtcctttcg ttccacctag ccattctgca acagttgctg ccattacagc atatgttgca
    34141 gatattttaa gtccagaaaa aaatttatcc attgccatag gaaatttaag gtgaatgaat
    34201 gtattaactt tatttgagtc catagtctta aataaattta aataatcttt atcaatattc
    34261 tcaatgccat ctacaaaact tattaatata ggaaaaaaac aagtaagcat taccatcaat
    34321 acttttggca ttgttccaaa tccaaaccat atcataaaga gtggtgctat aattattgta
    34381 ggtatcatct gtgtaaccaa cataatagga taaagacatt tttttattat tgatacaaaa
    34441 tccatcaata ttccaattac caatgatatt ataattgcga ctataaaacc aagtattgct
    34501 tctcctatag taaccacagc atgcatggat aaattttgat aatctttaaa aaacacatta
    34561 attatatcta ctggtgtggg tagtatatac tgaggaactt ctaaaactcc taccatagat
    34621 tgccaaagaa taagtataaa caaaaatgtt gctattggat atatactatc tttgacttta
    34681 tctatgattt ctaagttttt catctatagt cagacctcca gctttttcat ctactctagc
    34741 tactgttatg actctttcac atccaagatt aaaagggatt ttatgtacct tttttaataa
    34801 ttcaaaaact tcatcaaaat caccttcaac agtagtgctc attgctccta tttcacattt
    34861 aagaccactt ttttcaattt cagcaataca agcatctact actttataca tctcttcttc
    34921 acctttgtaa ggtcttagtg gcattactgc tatgtctgcg attaccattt aaatcactcc
    34981 tttaaaaata attaatacct actaggtata ttttgttttt tattaaaaaa ttttattact
    35041 aaaaagatat tatatttaaa taaaagaaaa gtcttatcca aaataggata agacttattt
    35101 ttatcttaac tggtctccct acactggcat tatccaaaca ggttcaaagg gtcgagataa
    35161 aatctcctct cagcacatta gctccccgaa atatattata tcttttctac ttgattatac
    35221 tacacttatc atattttaac aaatttttta tttatattag atattttatc cgttcaaaaa
    35281 tatctaatat aaattgtaca ttattacacc aaataaatta tcttaaaatg gtattttatc
    35341 aacatcttcc aaaactagat taactgaatt ttctagtttt tattttgcta tttcaagcat
    35401 atttggatgt atatcaattc catacaaata ttgtgctaaa tttctgtttt cactcatcta
    35461 atttgcaatc gttacagatt acagattaga aacagttttt agactctact tatctttatc
    35521 tacaaaaaat ctttcaataa attcattaat aatattcagt aatctaaaaa taaacctcta
    35581 tctggatttt taagatacaa accttttagg aagtatcttt tgtgataata taaaaattac
    35641 tcgtcattat catcaaaatc ctcagcaacc aacattttat ataagaatca aactctataa
    35701 tataattatt ttatccaatt tatttttgag aaataaaata tttttttaat ttttaaattt
    35761 tttaatatgc accttgaaaa aaagaaacgt tttcctatat tatggtaata gtagaataaa
    35821 actaaatagg caaaactaga gaaatttagt gacgcaaagc tatagggact aagacttatg
    35881 aaatgagtta tgtcagccag ttgccaaaga gatattttct ttttgttttt atgaatgttt
    35941 tttaggggga tatatgatat atgtttatgg ataaaatgga tagatgtaca catatactaa
    36001 ctgcatatat atgcagttca cgcgattact gcaattttat agatacacaa ttaaatgatt
    36061 ttatactaga atatggagag aatgtggtag aatcttgttt gcatcaagtg atggtattgg
    36121 taagcagata taattaaaga aattttattt tctagaaaac cctttttagt tttatctacg
    36181 ttttataatt cattaaagaa ctaattaaaa taagcttcat tatatatttt tcatgaagta
    36241 aacagttaag tttatctcat atactgttta ccccaattac ataattctaa taatatagat
    36301 tccaatgatt taccacgttt agtagacaaa tattcaactt ttggaggaat agtcggataa
    36361 gaaatcctct taataagtcc atctctttca agatacttta attcttgtga cagtatctta
    36421 tgacttatat gaacaacttc tttttttaat tgactgtaac gtattggctc ttgttttaaa
    36481 atatgccata aaataattgc cctccattta cttcctatca cagtcatggt aaaaccaatt
    36541 gaacaattaa aatctttttg tctttcttca atatttggaa attcttcaaa attattatct
    36601 atatccatct caatcctctc tttcaaaata gtaagtatct aactaaaaag tgcattcttg
    36661 tttagcgtac taattcgtaa tataatcttg attaaaaaat agctatgcac actcgtgact
    36721 ttagtcatgg gaggttcagt gtagcataga gccttacata atactacttt tttagatgta
    36781 tatgtttaaa ttttaatata gacatatgtt attttattaa ttttatttaa acagataata
    36841 ataaataata atttatctca agttttctat gtagataaac taatctaatg tttagaaagg
    36901 attatataat atgaataaaa aatttcgtct ttatcaagtt gactcattac aaaagaaaaa
    36961 tttacaggta atccagctgg tgtaattaca aatgctgatg gcttatctga atcacaaatg
    37021 caaaaaatag caagagaatt aaataattca gaaactgctt ttattttcaa taacttggct
    37081 aaaaatcatc aagaaaatta tgatgtacat atcagatttt ttacaccaac taatgaggtt
    37141 cctatatgtg gtcatgctac tattgctgcc cattatgtta gagcaataga aaaccaattc
    37201 aataacaaaa cagtatttca aaaaactggt gctggaatat taccagttga tattataaaa
    37261 aaagataatg attatgaaat aattatgaca caaggaaaaa ttgaagtatc tccaccatta
    37321 caacaaacct atcaaaaaag attacttaat gcatttggta tttaaaggaa gatattcgta
    37381 cagattgtcc tattgccata tcatctacag gacattcaaa agttatgatt ggaataaagg
    37441 aattatcaac attaaatagt ttatctccaa atttatatga attatcgaaa ataagtcaag
    37501 aaataaaatg taatggatat tatattttta cattacataa taaagaaact ctacaagttc
    37561 atggaagaat gtttgctcct gcaattggta tttcagaaga ccctgttaca ggaaatgcta
    37621 atggaccctt aggtgcgtac cttgtacatt ataaagtaat acctacaccc aacacagagt
    37681 ttatatttga agcagttcaa ggagaagcaa taaaacgcaa aggcaagatg ttagtaaaag
    37741 taattatttt agataataag ccagttgaag taaaaataat tggaaaagct gtaattgcat
    37801 tctcaacaaa catagaaatc taattataga ttccttctaa gtaaattttt gaacttttta
    37861 atactaattt aataacaaac taatttatat acaatcaaat taattttttt attat
//
LOCUS       DAYAPA010000026        37011 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.026, whole genome shotgun sequence.
ACCESSION   DAYAPA010000026 DAYAPA010000000
VERSION     DAYAPA010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 37011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37011
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.026"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            <1..2030
                     /locus_tag="ACGI59_001200"
     CDS             <1..2030
                     /locus_tag="ACGI59_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFG9739003.1"
                     /translation="KKLGIKTAIANAINGGKTLFIAPTGAGKSYSFINTLKKLKTKAL
                     FILPNASNVEQAMHEYDIPGAYDKIPVSEAFKNGNLVVMTWDKTEKLINTDLSEYIIV
                     VDEIHQTYTDAYRGKAIKNLNNIMSKCKGRIDITATPTKLEFEIYDYIIEYTQKQKTE
                     YKVKLYNDFDNKNFTEMINIINKSKNSAMLMNDISTLEFIRDSVNKKVGVVYADGKEE
                     NELYNRIVKNSDMKDYETLLNTTTILAGVNINNKDITDIIIVNIKDVGAIKQYVARFR
                     NLKKVNVHIFNKYEEECNVYKIEWLVNKNIEKATILKDAYNKVSKHMLQFETVGINAT
                     PIRIDSNVYYCTKDNCYKVDKLYIKSQVYSNYYNTRTIQSFKVLLEEYFENVNITDAK
                     EVGTNEKELKEYKKVVKEIKDATREILKEHKEILVGYRQIKSDSKSFSLMQYHNDMKL
                     SSKGCLEAYRAYDIHNLVKKSKSNSMLELYSNYVLDNKFDLDLAWKLANTANKRRGVI
                     FNKINTLIYRELKEEYPEFLNDELIQVTVFNYIDKLFGIGTSYKELHLQELSNDLRTI
                     LGENWDLTTKKLSVTLNGIFIIEKKKYSNWHDIETMFFYKNINPISCQFEKKRIQLNN
                     IKRYITVDDIKKDLELDEKDKSLENAIKYTKDRMLNSLDEDEKLLLLKGFM"
     gene            2077..2424
                     /locus_tag="ACGI59_001201"
     CDS             2077..2424
                     /locus_tag="ACGI59_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9739004.1"
                     /translation="MFELDIKVKNKYIVSVSYEFSELLIRAFNDYDNFIKDIKNKDKF
                     IPIGINLGYRELLEENSAYHEYNFYYDEEVDKYFFELQYQEQDLREELSKQLLLELIK
                     INLPAGAELIVVD"
     gene            complement(3250..3603)
                     /locus_tag="ACGI59_001202"
     CDS             complement(3250..3603)
                     /locus_tag="ACGI59_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFG9739005.1"
                     /translation="MNTRIRDLRIAEGLSQIEFGNKLGMTRHEIYNLESGRTRIKESD
                     LKLITSTFKVNEKWLKTGEGDMYTFSNNNLITAEVLCAIDKNERLAKVMLKFSKLNDK
                     ELEAMEKLLELFQKE"
     gene            3776..3958
                     /locus_tag="ACGI59_001203"
     CDS             3776..3958
                     /locus_tag="ACGI59_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9739006.1"
                     /translation="MTREERILRNVILYLDMSEEKQRIVDCILNAKTIENIVENDILD
                     VNKLNEAVKEIEQIIC"
     gene            4027..4629
                     /locus_tag="ACGI59_001204"
     CDS             4027..4629
                     /locus_tag="ACGI59_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9739007.1"
                     /translation="MEINSVRELRELFELFTVIKAEGNNVVSCNETVNELSNKELIKA
                     NENKFKKPWNDALLYEINALDIIKKHKHSDRKEKAYKTYLGLENADIEEIYKIHYGDK
                     ENSETVASCEEISSAKTDTISCNHDSVSKGKDTSDSECTCFICSKKIENQEDVFKMPF
                     LTRDYLSSAKEIKNIEICEKCLDGNSWSIIRRKQFKKMKK"
     gene            4675..4956
                     /locus_tag="ACGI59_001205"
     CDS             4675..4956
                     /locus_tag="ACGI59_001205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9739008.1"
                     /translation="MIKNNVLSYEEKIKVLKKHGYSDFTKEYVLKEFDKEHAFYKSFL
                     RAIEKEDDGKGVLIKKIGKGQHQFSKGFIKTHTISELLEEAKRIRKILE"
     gene            complement(4995..5339)
                     /locus_tag="ACGI59_001206"
     CDS             complement(4995..5339)
                     /locus_tag="ACGI59_001206"
                     /inference="COORDINATES: protein motif:HMM:NF022172.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2513 domain-containing protein"
                     /protein_id="HFG9739009.1"
                     /translation="MVTNQDCIRDILLLIESNTTPISPVLQFDTILDNLTYDKSTIVH
                     HINLIYQNNLVDDVNYGDDTVLEVMQLSLKGYQYLDKIRDNTKWNKIKSSTSSFLNMS
                     LPIAIEYILGKF"
     gene            5428..5604
                     /locus_tag="ACGI59_001207"
     CDS             5428..5604
                     /locus_tag="ACGI59_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9739010.1"
                     /translation="MQKRITEMSILEKVDLIGQKQKKLQEKYKNLSKGELFSIACNEV
                     KKIEEQEGKYFEYR"
     gene            5591..5956
                     /locus_tag="ACGI59_001208"
     CDS             5591..5956
                     /locus_tag="ACGI59_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9739011.1"
                     /translation="MNIGSYTLVKKQLIEELKEEIKQKEENLKEREKYIKELKERLEC
                     FARDALESEECIIDIENILIEQTVELEAYNLFIDCILNKDIRKAIAIYNRTKSIRIKH
                     KNINSISKKIEKIKQIISL"
     gene            5976..6149
                     /locus_tag="ACGI59_001209"
     CDS             5976..6149
                     /locus_tag="ACGI59_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9739012.1"
                     /translation="MDKFVTCARCERTINVEENNHVKYEEETLNLSFTLYFCLGCVDK
                     LIEQSKMLEGENE"
     gene            6142..6357
                     /locus_tag="ACGI59_001210"
     CDS             6142..6357
                     /locus_tag="ACGI59_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9739013.1"
                     /translation="MNKISVTQALEKFDSLLDNWNDLPNHVYTLEYRSKFYEWIKLLE
                     RKGSLQNYKIVEVLNAERNGEEALFWN"
     gene            6416..6589
                     /locus_tag="ACGI59_001211"
     CDS             6416..6589
                     /locus_tag="ACGI59_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3797 domain-containing protein"
                     /protein_id="HFG9739014.1"
                     /translation="MKMINVFKLSSMYCICPKCGSDKLGEGEGKLIVDEHTYYRECKC
                     GFNVLVDEREDEI"
     gene            6604..7011
                     /locus_tag="ACGI59_001212"
     CDS             6604..7011
                     /locus_tag="ACGI59_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFG9739015.1"
                     /translation="MNSVILIGRLTKDPELKYIPGSGTAVSTFTIAVDRDYIKKDGTK
                     ETDFIPIEVMGKLAEVCANNLGKGRLVAVEGSIKVNSYEKDGEKRTYTKVHANKIKFL
                     DYKKDDTEKEYMFEPKGLDQQGFQAIDDPNIPF"
     gene            7028..7177
                     /locus_tag="ACGI59_001213"
     CDS             7028..7177
                     /locus_tag="ACGI59_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9739016.1"
                     /translation="MSNTNKKTAKICGWCGTTVYSYMDGKIAYCSKKCKKKREKNLET
                     EGAKK"
     gene            7174..7422
                     /locus_tag="ACGI59_001214"
     CDS             7174..7422
                     /locus_tag="ACGI59_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9739017.1"
                     /translation="MKEYRIEIGEMIFLKHKVIVEVPEQICAFDIYKYIENECQDKSE
                     ISEYIKSIGGRQLNFIEGDSGDSELIVGSCKKIKKGEK"
     gene            7424..7714
                     /locus_tag="ACGI59_001215"
     CDS             7424..7714
                     /locus_tag="ACGI59_001215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9739018.1"
                     /translation="MKKTKIEEFITRVKESMQSVAKESIKDLENILKEIETGETEVKI
                     LFIDKGKKVEEIVEFTHDEYVKMIIEKMIFDSLKIIKEQHEKILKEFEEREV"
     gene            7717..7977
                     /locus_tag="ACGI59_001216"
     CDS             7717..7977
                     /locus_tag="ACGI59_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9739019.1"
                     /translation="MNFKMIANIPKWTVKEQFEKVLEEVLELKEAIELKDNKKIMEEG
                     LDVIQAIFTLFKVLKIDKEIREGLKLHNKKLRKRKWKLEKIN"
     gene            7995..8204
                     /locus_tag="ACGI59_001217"
     CDS             7995..8204
                     /locus_tag="ACGI59_001217"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9739020.1"
                     /translation="MSICRKCLYGDYLDELVVGECILCGQEFFSIKTPVGKMCPSCSE
                     MFKGNCSQCAKPIGNEKKSFKNKLK"
     gene            8447..8908
                     /locus_tag="ACGI59_001218"
     CDS             8447..8908
                     /locus_tag="ACGI59_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9739021.1"
                     /translation="MKIKKEYFDKVENALKMLGDKYRKLRVLKKEIAILKHKENYREI
                     NYEELGFKVQKSVKGIDDMVVTIEDAIYNKETEIEIIERKLEFYNIYLKELSEIEQKI
                     IELVYFYSWNGKMPITKMAMELNYDRTSLYNKKAIAINKIALMIYGDEALE"
     gene            9088..9636
                     /locus_tag="ACGI59_001219"
     CDS             9088..9636
                     /locus_tag="ACGI59_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFG9739022.1"
                     /translation="MNFVEPIRNLEKLESICAYLEETNMRNNVLFSMGIYTGLRVGDI
                     LKLRIKDVHDKRFITLKESKTGKTKPIEVNPILRRILKEYTKDKNQNEFLIKSREQTN
                     KAISRVMAYKIIREVGEMFGIDNLGTHTMRKTFGYHYYKRTHDVVTLQKLFNHSSATI
                     TLRYIGIEQDDLNKAYRNIKYF"
     gene            9809..10081
                     /locus_tag="ACGI59_001220"
     CDS             9809..10081
                     /locus_tag="ACGI59_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9739023.1"
                     /translation="MNKYGKFKCNNCGKKVNVNNRDLKTKVIDNTTVTYFMCRRCFEK
                     FVVSCEDDYIKHKREEYKKLKDDEVKLLQDMKEYSDGLGELYINEL"
     gene            10071..10403
                     /locus_tag="ACGI59_001221"
     CDS             10071..10403
                     /locus_tag="ACGI59_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFG9739024.1"
                     /translation="MSYKHMTLDRLKELILIDRLDIFYRSTDWRKLRKKALERDNNEC
                     QICKEKKKVSKAECVHHIKEVKEYPGEALKLNNLTSLCNLCHNEIHEKILKINQEKKK
                     KFFSEERW"
     gene            10575..11081
                     /locus_tag="ACGI59_001222"
     CDS             10575..11081
                     /locus_tag="ACGI59_001222"
                     /inference="COORDINATES: protein motif:HMM:NF016975.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HFG9739025.1"
                     /translation="MDDNEELEIRRDVQNDYVLGMTYKEMSAKYNIPENTLKSWRKRG
                     GWTRNCTPKKGCVKNGLQESGNNLKNSIRKDLLRQIKENNMTEEYFKDLVEDYLKMWD
                     IKNELIEDVKTRGVQVKYKNGQNQWGYKKNDSVSELIKYNAQMLSLLDKLGIKATPKV
                     LGDDDIEL"
     gene            11071..12693
                     /locus_tag="ACGI59_001223"
     CDS             11071..12693
                     /locus_tag="ACGI59_001223"
                     /inference="COORDINATES: protein motif:HMM:NF042863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFG9739026.1"
                     /translation="MNYKYNKYIDEYFNLVDKGEIVLCKEMELAVKKIKKDLKKPNIY
                     IDHEKVEKAIEYMEKYFYKMYAWEKFVVALVVGCFEGDELLYDEIFIMMGRGNGKNGF
                     ISQLANYLQTHLHGVKNYGIDIVAMSEEQAKTSFDDVYNMLSEHPAICKKFFNRTKEV
                     ITFKKTNSYLKFRTNNAKSKDGLRPGCIIFDEVHAYENYDNIKVFTSALGKVKNPRVF
                     YITTDGNVRGGVLDDFKEESKQILNEEIKNSKMLPLIFKLDSIEEMENIDLLEKANPS
                     IKYNSDLRTQILKDIDKAKNRPQMMIELLTKRFNLPAQNLATVVAEWDDIVATNEELP
                     DLKGFSCIGGLDFSSIRDFTSVVLLFKKDKKRYIMHHTFICYKSLEITNFKFDIELAK
                     QKGLCTIIYDETIKAEYIADWFIEKAEDYNILKICCDSYRAAHVKEEFANKGLPIEEV
                     RNGSITHAKIAPLVEQIFAERTIKWGDDMMMRWYTNNVYVDTDKKGNKTFLKIEPIKR
                     KTDGFFAFLHALSKDGELMQESTIKFYDCYTY"
     gene            12712..13902
                     /locus_tag="ACGI59_001224"
     CDS             12712..13902
                     /locus_tag="ACGI59_001224"
                     /inference="COORDINATES: protein motif:HMM:TIGR01537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFG9739027.1"
                     /translation="MNFKNFITNFLFKDKMSGADGEYFKKLEATIFYKEFALRSCISI
                     IANALVLSEFQTFINSKSVKEKNYYLFNIEPNQNQNATEFWQEVITRLVYDNECLVIQ
                     ENEQLFVAESFNVDKYVFYEDVYKNITIRNYKLDRSFRESDVLYFKLNVESIKTVIDY
                     LYNDYKDLLSSAMKGYKKNNSEKGVLEIDTNYPQTKEAQERLNDLMSNKFKRYFESDN
                     AVLPLSNGLKYTENTKASSIKDSRDIRAIIDDVMDFVCAAFHIPAGLIKGNVSGVEGI
                     TDNFLNFSVNPIARLITTEITRKMYGRDKFFEKSYVKVDTQKIANMGLEKISKASDLL
                     FRIGVNSINDNLEMLGREKINESWADEHYVTKNYQSILNPDLKGGANNGNGEKSGIAG
                     NKGQ"
     gene            13856..14491
                     /locus_tag="ACGI59_001225"
     CDS             13856..14491
                     /locus_tag="ACGI59_001225"
                     /inference="COORDINATES: protein motif:HMM:TIGR01543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFG9739028.1"
                     /translation="MEMEKRAVSLEIRDSNLESRKISGYAAIFNDDYTKLQDRWGDTF
                     YEKISRGAFLKTLADNTRDKFMLINHDWNKVIGRTNSNLILEEDERGLRFELDIPATT
                     DGNDLLENVRLGLIKGCSFGFNIVNSKTRFDDDWTYYRDITEVELFEITATPIPAYND
                     TEINCRSDISIKELRESQKEKVKQNDNKDNIEKRNVDLVSAFFNAFNRGGK"
     gene            14495..15661
                     /locus_tag="ACGI59_001226"
     CDS             14495..15661
                     /locus_tag="ACGI59_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFG9739029.1"
                     /translation="MAIKNLDEATNVELRTNLMEAIKSNDEEKITTSFLRMAEDIQSN
                     LLKEARSIANIESADRAILDKRGMPQLTSEERAYYSQVIEKRGFTDLEVTMPRTIFDR
                     VFDDLEQNHPLLSAITFENTTGVTEWVIRKTGTEAAWWGQLTDPIKKELEGGFAKINT
                     VAYKLSSYLPVAKSMLDLGAEWLDRYVRVVLTESISLGLEMGIVAGTGKDQPIGMMKD
                     LKGSVVEGVYPDKTAKPLADFKPTTLGTEIMAPLTNDGKRTVASVIMLVNPVDYWAKI
                     FGATTMLTSNGTYVYGVLPIPGNIIQTVAVPQNKLIVGVAKDYFMGIGSAGKIEYSDE
                     YKFLEDVRVYLAKQYATGTPKDNFSFLVFDIENLDTTLATEINSNTKTSAARTK"
     gene            15671..15958
                     /locus_tag="ACGI59_001227"
     CDS             15671..15958
                     /locus_tag="ACGI59_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9739030.1"
                     /translation="MENENIKSIVEEVKEYLNVTWIEEDAKIEKMVVRGIDYFENEIA
                     GIALDFKDNFNRELLFNYCRYVRNNAIEYFEENFSKELLRLQIKSAANEVI"
     gene            15961..16611
                     /locus_tag="ACGI59_001228"
     CDS             15961..16611
                     /locus_tag="ACGI59_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail adaptor protein"
                     /protein_id="HFG9739031.1"
                     /translation="MKKIKFNNFNDGIVEFGEYKESYDIEGNALDEKEFIVSGKLFYS
                     NEYIREQDKLKFDNTGLKVSIKLKVPYMRLIKTSDVIKLNNEFYSIVKLDISNDKTSI
                     YLYLSELVDVLDKHISIFIKERKNVLEDVAMKFYKKIWACVEDLNSKELIENNSIKTS
                     TNKKFKIRYIEELDLSIKQNAITNFVIKYKDKFYNITQILNLEEENKILEITAESK"
     gene            16631..16957
                     /locus_tag="ACGI59_001229"
     CDS             16631..16957
                     /locus_tag="ACGI59_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9739032.1"
                     /translation="MHSKIVTILKKLNLGIGFQEYDNFSNDEEYIIFFIENEYDANFS
                     DNENKSIVYSIKIEYWYKSLKNINKYISIKDIFKKENFIFLNSSDFIENEYYCKELKF
                     KYEEVV"
     gene            16958..17401
                     /locus_tag="ACGI59_001230"
     CDS             16958..17401
                     /locus_tag="ACGI59_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFG9739033.1"
                     /translation="MGVEITTEGFDAILSKIESMGKSGDKLLNEAVKAGGNVILQDAL
                     PRVSKRSGKLKDGLKVSGVKKKGGTKYVLVGITKEDNSKIFYGKFLEFGASAHQIPIK
                     KGKKKGRVINHPGVSPKPFLAPAYESKKDEAKNVMKEILKRGLGL"
     gene            17401..17736
                     /locus_tag="ACGI59_001231"
     CDS             17401..17736
                     /locus_tag="ACGI59_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9739034.1"
                     /translation="MININNLVIETLKPLKIPVSFQRYNGEEKTYITFFSYLEQNFYA
                     NDEVVGTEHYIQIDLFSKNKMSYINKEIERLLKKNNFIKRSIHEIKESDYSYHTVFRF
                     LFFAKNEED"
     gene            17740..18336
                     /locus_tag="ACGI59_001232"
     CDS             17740..18336
                     /locus_tag="ACGI59_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major tail protein"
                     /protein_id="HFG9739035.1"
                     /translation="MAIIGLRNYRIVKLLTDTEETLEYDTKITRLTGAKSVKISPKVD
                     SAENYGDDQLLETASAMGAIEVEIEVADLTLEERALILGYQYQDGVLIEDKNFNPPNL
                     AFGFESPKSQNGSRMVWLTKGVCEPFEEEAKTKEDKIEFQSQKIKLKFMPRINDGRHK
                     ITADTDVENAPTDVEFFTTEFLKTGAKPTAPAKANLNK"
     gene            18354..18641
                     /locus_tag="ACGI59_001233"
     CDS             18354..18641
                     /locus_tag="ACGI59_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9739036.1"
                     /translation="MEIKLTINEEEKIFKAPPIALKKLDNAFALSDKINDGLSMRGLF
                     QELLDFTIDIYGEQFTKEELLNGFYPADEFMSKALEDLSKVSGTFEEKVKN"
     gene            18725..18880
                     /locus_tag="ACGI59_001234"
     CDS             18725..18880
                     /locus_tag="ACGI59_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9739037.1"
                     /translation="MFLFEKNWSMVDIDNMDIFYYLDILAYMKKNKSDKNNPNENDVY
                     IDQLSWL"
     gene            18893..19108
                     /locus_tag="ACGI59_001235"
     CDS             18893..19108
                     /locus_tag="ACGI59_001235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9739038.1"
                     /translation="MCKYCEVEESEGAFKRGKTDRGELITCNCDIEMWIEKEGNEYFL
                     NSECWCGYYSESFEPINFCPFCGRTLI"
     gene            19171..24372
                     /locus_tag="ACGI59_001236"
     CDS             19171..24372
                     /locus_tag="ACGI59_001236"
                     /inference="COORDINATES: protein motif:HMM:TIGR01760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFG9739039.1"
                     /translation="MAEDVGELVVRVAMENSSFQQGINNLNRQMKLIRSEFKNSGAGV
                     KNFGTSLDGLKSKQQMLSSTIQQQTKVVEAYKNKLNESKTTLSNTAQKQVELKEKVNQ
                     AKSAYEQSKATLGENAEETKRLKSELDSLEQEYAKNEEKIRSNSAAVDNWSIRVNNAE
                     SRLSEMRSELDDTTERINQQENKWNQLATKMTEIGNKFSSVGKQMQDVGKKMAMSITA
                     PVLAVGAAASKLGMDFEASMSNVQGLSGATADEMVQLEKAAREAGASTSKTAKDAADA
                     LGYMALAGWDAKTSMEALMPVLRLSEAGNLDLARTSDLVTDSMSSLGLSVKELPTYLD
                     QVAKTAASSNTNIDALMEAMIVSGGTFKNLNVPLSEANALLGILANRGLKGSEAGNSL
                     NSIMINLTSGAGQAGVAMKELGLSAFDSNGKFKGMANVLMELKEKTKDMTEEQRTMYL
                     SMIGGKTQISTLQALLSGVGEEYSELEKKVGNSTGALDKMAKTMQNNNKGSITALMSA
                     VEELGLKIYDVLKPSIAQAVSWFQNLTNKLNEMKPSTVETIVKIAGLAAALGPVLLIS
                     GKIVSMIGGVIGAFGTFSAAMAVVTTGAAAATPAVGALAAILGALSSPAAIAVAGITA
                     LGISVATVAKDMSKDAIEPISRFGKSISESTKEAVSAFMDLEEKTTVSLNQLMWSGET
                     VSEKMKQTIVSNFTEMSNQIVAKLQESKEQGIRSLQEMFATSKNLSDKEKEELIKNTE
                     EAYSSKEEKIKKSNEKVNQIMTKASQENRALTKEEATEINQIKTDMLNTAVDTMSKSE
                     AEQAAIMERMKANHVDLSAKEAAEVVKNSIKKKDESIKAANEEYNERLKIAAQLRAEG
                     GAENEKLADKVVEEATRQKDESINKAKEMHQGVVAEAQKQAENQASKVDWTTGEIKSK
                     WQVLNDTVKGKMQEWDDMAGNWVEGLKEKISTGWANLKENTAILWDNIKLTVTTKWTE
                     IKESIAFKIEEIKQSISEKWESIKLFFTTTWENIKMIFGNAWDAIKQQVEEKFAPFVE
                     RLKIIFDGIKEFFRSTWEVIKNIVLGAVLLILDLVTGNFDKLKTDAIQIWENIKQSLS
                     DAWESIKEVFNNALTAISDLLSNMWEGMKSTASSVWESITQTLSSIWENIKSTCSNAW
                     EGLKTICSNTWTNIKTTLVTSWENMKTAVSTKLTEIKNNIVTVWNSVITFFKTLPAKL
                     KQAAIDMFTRLKEGIDSKKQDAVNASQKVGTDVVNKIKEFPSKFLQVGKDFVSGLANG
                     ISGFAYKVVDEAKKLGQKAADAVRKVLGIHSPSRVMAEIGKFVDEGLAQGIANNSDSV
                     KKATEKITKIIEEETKKATKACEEDIKLFNKQIDELSKQEKAALKKVKGDAKDALKEE
                     YAAKKEKIKEAIALRKEQKEKEVEQLKEIANSAKEQLNKELQDRQEFVKKVDDLTKAI
                     VDALKEKYKQEYEAQQASIKKELDALDKWKEESIDRINSVYDAKIKAIDNELEAFEKA
                     EKEKDRLEQDKEELNKIEEIKTAIKFEHDDTNKEQLQKELEKVLAERKKRIEQQEIED
                     KKEALKKRKEELEEKKKDEIENINQIYESEKEYYNKRLEDAKKFYDERIKESKLQAEA
                     EKLIMDKNQKEIVALLNSYSDAYKNAGQTLGEKLLEGFKPAINEIKNLIDSITREINN
                     ARNEALSLNSDIRKGNSAANSNSGRREATYNNNSRTANYNVNVNSTDSNTAREIERTI
                     RSLAFSL"
     gene            24404..25270
                     /locus_tag="ACGI59_001237"
     CDS             24404..25270
                     /locus_tag="ACGI59_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail family protein"
                     /protein_id="HFG9739040.1"
                     /translation="MQKLICRNSKGQEVKLGNSRPFLLEKIENGANTSANISVSNNLG
                     DGVSIDDISIKERLLPISAAIYAKNKEDLELKRAYLTSLFNPKLGEMEFIYTNNALTK
                     RVKGVVQDITFQTPVGLMQKFLIQLLTPSPFWMDEFVLKEEVALWVGDFEFTLEISNE
                     GIEMGHRVSNLICNVVNNGDVECGMKIQFKALATVDNPSLFNVNTREYIKINRTLEAG
                     DLLEITTEFANKRIELVKSNYERINVFNWIDLDSEFLQLEVGDNLFRYDADVGIDNLE
                     MSIYYNPLYLGV"
     gene            25272..26351
                     /locus_tag="ACGI59_001238"
     CDS             25272..26351
                     /locus_tag="ACGI59_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siphovirus ReqiPepy6 Gp37-like family protein"
                     /protein_id="HFG9739041.1"
                     /translation="MQQSIRILDKNINLIAEIDNYEELQIIKKFHKVGEFSLKINANK
                     NHVDKLVKNNIILLGKNYNKVCLILHREFMYTETGEKSDILSVKGVDLKGLLNRRLII
                     PDTGEAFESHEGTQEEIIKAFVNNNCVDSSNPKRVIDNLIISENKNLGSADMWRSSYE
                     NLSDKIQEISEFCGLGWEVVLDANKKKFIFDVITGKDLTINQTENPPVIFRSDFNNIR
                     ARHFTESIINSKNVVYAGAREDATKLVISTGEVEGFERNEVFADVSENTISILQKEGE
                     IKLKEYEELKSFELEIDPKNTFMYEKDYKLGDIVTVQDRKLKVTMDTRIVEVQESYSK
                     NGMKLKITFGSSIPTLFSKIKRMVK"
     gene            26351..27238
                     /locus_tag="ACGI59_001239"
     CDS             26351..27238
                     /locus_tag="ACGI59_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9739042.1"
                     /translation="MEKSFVFNSVNGDRRYKAEDFREYFASFIGNGVFPNPSSNLQVV
                     DNNNNNMTITVKKGKGWINGAIYINTDDLVINIDPADGILNRIDRVVLRFDTLNRNIK
                     LAVKKSAFNSSPAATELQRDADAYELGLADIYIRAGAISITQSSITDLRLDKNLCGIV
                     KGTIEEIDTTTLLAQLSTWKDEEISNFNDWRENQENKQEDWYNTTTTNFVSEFNIWFE
                     SIKDILDEDAAGNLLNEINKVKEELAKIETTAEKTSYNNATSNLTATNVQGAIDEIVA
                     KIENFNEINISIQNDMLPI"
     gene            27255..28943
                     /locus_tag="ACGI59_001240"
     CDS             27255..28943
                     /locus_tag="ACGI59_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine rich domain-containing protein"
                     /protein_id="HFG9739043.1"
                     /translation="MAIVYEFNYTGAEQSVVLPPGKYKFECFGACGGNYYDFVQCAKG
                     AYTAGSLILKENTTLHVYVGQSGYCKGVNGIETCRSGFNGAGGITTYKSTSDGYYSLA
                     GGGATDIRLIGGNWDNLQSLLSRIIVAGGGGGGSGNSHDSIGHGGGTKGKDGISIANK
                     YFAGGGSQFQGGLTFNSLYNGSFGVSGAGDGISGVGGGGGWYCGAGSFYAEFGGGGSG
                     YILTKDSYKPANYSPSSKYYFSDINSVVGGNTTKQDGYAKITLLQALPFLTISSYNST
                     TATFKADHTDPTLLTKIEYFIDDVLKETITTDLTLEKTINYTLEDNALHTLKIVVTDS
                     ANATAEKVVSISKGIAPLPAGSTTDEVTSKWIEIKDAFKSGKTSIINTLALKNIEASL
                     NNTLVELSEKIKTSFDSSDASVQDLMNQLTQANNTISQLNTRYKVASGITYQLNNPSL
                     SANFYNGGYTTTQDHWINVSNLGFVPHIFIAECDFTKDGYLTKSLVFASYNVFSKDYV
                     ISSYFRRQTNSTFYSHGNIYNLNEKDVYVNGRGVQLPAFNNYDFAYKWQAIKFV"
     gene            28957..29250
                     /locus_tag="ACGI59_001241"
     CDS             28957..29250
                     /locus_tag="ACGI59_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9739044.1"
                     /translation="MNRANRIIYDQTGKMLLQTGEATGDVLEYDTITELHCIDIPYGS
                     IDYTKNRIIGINIETKEPILEEIPTFISDEEKRIQELENQLLIAENEKVGGIL"
     gene            29250..29432
                     /locus_tag="ACGI59_001242"
     CDS             29250..29432
                     /locus_tag="ACGI59_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015981517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9739045.1"
                     /translation="MNINNVVVRILAERILNGGLNPLKNREFELDDVTNAEYRKAVED
                     YIIEHSGVVEGTQPTK"
     gene            29495..29926
                     /locus_tag="ACGI59_001243"
     CDS             29495..29926
                     /locus_tag="ACGI59_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFG9739046.1"
                     /translation="MNVTIVFLATNIFIKLVILAIAFDTLLGCLRAIKTHQFNSSFGI
                     NGGIRKVAMIACIFFLAVVDILTKFNFLFMLPQDWVDFLRLNHLGISEFFSILFILYE
                     SVSILKNMYLCGLPVPKRLKEKIGSLLDTMTDELNVKGGSK"
     gene            29926..30765
                     /locus_tag="ACGI59_001244"
     CDS             29926..30765
                     /locus_tag="ACGI59_001244"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFG9739047.1"
                     /translation="MKICITVGHSILKSGACTSANGVVNEYQYNKSLAPVLADTFRKE
                     GHKADVIICPEKQFKTKAEEKIYKIPRVNSGGYDLLIELHLNSSGVGAFGTEVFYYSE
                     KGKEYAQRVVDKLSKPFIRKKGDKEVGNRGVKLDKSLYILNSSKPTAILIESFFCDNK
                     EDYEKAKKLGHEGIAKLIVEGVLNKNINNEGVKQMYKHTIIYDGEVDKIPATVVGWGY
                     NDGKILICDIKDYVPGQTQNLYVVGGGACEKIGSITKEKYTMIKGNDRFDTLYKALDF
                     IDR"
     gene            complement(30816..31028)
                     /locus_tag="ACGI59_001245"
     CDS             complement(30816..31028)
                     /locus_tag="ACGI59_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9739048.1"
                     /translation="MTWKNEIKSILVKENISMNELNNLMNEHNNKNNTVENLRQKINN
                     ETMKYSEILTIANLIGYEVVWKKKEK"
     gene            31207..32037
                     /locus_tag="ACGI59_001246"
     CDS             31207..32037
                     /locus_tag="ACGI59_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9739049.1"
                     /translation="MKTLFKEAHKIAREIKEKYNDVDYKVQFSLCLSFLNKKGDIKMK
                     ELKGSEKQIIWANDIRADILNLTNELEKSKLERIKNEDYKIKDMSIEEMIERCKRKFE
                     RIREAISNIEDAKFFIDNFRNVLKYNSLNQKAFQINQIFRESQFTEEIGNLKFLKGET
                     QASHKLRKVEGSISYEEAKKIAKEINFNDNWSNEIKENVLEIVEIFDDAVKQATSTEY
                     IEKAKKYIIETLIQKNAQFYKNGFADIKKKQIENYRKIESAERKIEIAYQIFRDGVEC
                     "
     gene            32116..32622
                     /locus_tag="ACGI59_001247"
     CDS             32116..32622
                     /locus_tag="ACGI59_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9739050.1"
                     /translation="MNIDFSTILNLKVNKENVEMIEKEINKINNDIFDISLKDCANSH
                     VGIVKETVGKSLNIETWGHGEINSSIGIRLRFNFYYSKGDKVDIENIENILIYVNSLC
                     SKNVESDLIASVRPGFYKLEEKYSTRVEKCEIRHEFTGINKQEIKEFLEYLTEIEKSE
                     RTVMYKNN"
     gene            32642..33112
                     /locus_tag="ACGI59_001248"
     CDS             32642..33112
                     /locus_tag="ACGI59_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9739051.1"
                     /translation="MNKQKARRFLRVIDMNIDKIEEEAIKAFKESCLIKETNNIKIYI
                     DIQGKVEAIAVQTWAKLLGDDKEINIFTLNQAPTHLNDMLGEICYVNDYEEFENWCEN
                     EWENLDWDSYKKFNKENFEEIAERNIDDSTSVFLEELQKGIESCKQELQNVIEN"
     gene            complement(33345..33626)
                     /locus_tag="ACGI59_001249"
     CDS             complement(33345..33626)
                     /locus_tag="ACGI59_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9739052.1"
                     /translation="MNVYLQIGKELSEKMTKSELNKFSQEVTNYLRFAGHDNDMKIKL
                     FQTIIEAIARKRVTLPADNEFTQGIMLSNELEMTTNVGFIIEGLSYHNN"
     gene            complement(33770..34102)
                     /locus_tag="ACGI59_001250"
     CDS             complement(33770..34102)
                     /locus_tag="ACGI59_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016040102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9739053.1"
                     /translation="MPSFNRDLLVDKEKPILKNGTIVQNDSTTTDNKTLNIYNLVNKK
                     SKKVTMSATLDEELVNKLKSFSIDMNSDVSKLLSDILTQILSDVTIKEENLNIYNERN
                     RRNKTKKK"
     gene            complement(34103..34912)
                     /locus_tag="ACGI59_001251"
     CDS             complement(34103..34912)
                     /locus_tag="ACGI59_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFG9739054.1"
                     /translation="MANTKLLTYFNIKGGIYKTTTSIMTAYELAKDKDKKILLWDLDV
                     QANLTQYVYEINHNDNTTLDILKGISANDAIVKSPNENYINVDLIPSDIQMARFEQEL
                     SPLPAREKFLARWYMQNFNTLSEYDYIICDLSPRYDLTAKNVLFLADSIIIPIQDKNI
                     SSLRGAELFKQLWDVDRTYFDKEDNIKSTILVGFEKKKTQISDTFDSYLEGFNDMRDI
                     MLDTYIRKNEFIEKALLKKLSLTDYTKITKEHFSRQEFTNMLEELKRKGVL"
     gene            complement(35201..35383)
                     /locus_tag="ACGI59_001252"
     CDS             complement(35201..35383)
                     /locus_tag="ACGI59_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HFG9739055.1"
                     /translation="MEKEKRDLKFNFHKGGSGSFTPRMSVPKKWADEMGITRENPNVT
                     ATFEGDRIIIEKAKNE"
     gene            complement(35623..35751)
                     /locus_tag="ACGI59_001253"
     CDS             complement(35623..35751)
                     /locus_tag="ACGI59_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9739056.1"
                     /translation="MGRLERSKKKRENKFNIVKKVFSFILLLLNIILAVLRILKEL"
     gene            complement(36204..36356)
                     /locus_tag="ACGI59_001254"
     CDS             complement(36204..36356)
                     /locus_tag="ACGI59_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9739057.1"
                     /translation="MDNFLLNVLAGVIASLIFYIISKLFRQVKNHSAQKSGWEFDLRI
                     KFKKFK"
BASE COUNT        15233 a         4128 c         6630 g        11020 t
ORIGIN      
        1 aaaaaaaact aggaattaaa acggctatag ccaacgcgat aaatggcggg aaaaccctgt
       61 ttattgcacc gacaggagca ggaaaaagtt atagctttat aaatacatta aaaaaattaa
      121 aaacaaaagc gttattcata cttccaaatg cttcaaatgt ggagcaggca atgcacgagt
      181 atgatatacc aggagcatat gacaaaatac ctgttagcga agcatttaaa aatggtaact
      241 tagttgtaat gacttgggat aaaactgaaa aacttataaa cacagactta agtgaataca
      301 taattgtagt tgatgaaata catcaaactt atacagatgc atatcgagga aaagcaataa
      361 aaaatctaaa taatataatg tctaagtgca aaggaagaat agacattaca gcaacaccaa
      421 caaaacttga atttgaaata tatgactaca taatagagta cactcaaaag caaaaaacag
      481 aatataaagt taaattgtac aacgactttg acaataaaaa ttttactgaa atgattaaca
      541 taataaataa atctaaaaac agcgccatgt tgatgaatga tatatcaaca ttagaattta
      601 taagagattc agtgaacaaa aaagttggag ttgtatatgc agatggaaaa gaagagaatg
      661 aattgtacaa tagaatagtt aaaaattcag acatgaaaga ctatgaaacg cttttaaata
      721 caacaacaat cctagctggt gtaaatataa acaacaaaga tatcacagat ataattattg
      781 ttaatataaa agatgttgga gctataaaac agtatgttgc aaggtttaga aacttaaaaa
      841 aagtaaatgt acacatattc aataaatatg aagaagagtg caatgtatac aaaattgaat
      901 ggctagttaa caaaaatata gaaaaagcaa ctatcttaaa agatgcttat aacaaagtat
      961 ctaaacacat gttacagttt gaaacagttg gaataaatgc aacgccaatt cgcatagact
     1021 caaatgtgta ctactgtaca aaagataatt gctataaagt agataaatta tatataaaaa
     1081 gtcaagttta cagcaactat tacaatacaa gaacaattca aagttttaaa gtgttattag
     1141 aagaatattt tgaaaatgtt aatataactg atgctaaaga agttggcaca aatgaaaaag
     1201 agttaaaaga gtacaagaaa gttgtaaaag aaattaaaga tgcaacaaga gaaattttaa
     1261 aagaacataa agaaattcta gtaggttata gacagattaa aagcgattct aagagctttt
     1321 cactaatgca gtatcataat gatatgaaac taagttcaaa agggtgctta gaggcttaca
     1381 gagcgtatga catacataat ttggtaaaga aaagtaaaag taatagcatg ttagaactat
     1441 attctaatta tgtactagat aataagttcg acttagacct tgcttggaaa ctagcgaata
     1501 cagcaaacaa aagacgtggg gtaatattta ataaaataaa tactttaata tatagagaat
     1561 taaaagagga atatccagag tttttaaatg atgaacttat tcaagttaca gtatttaatt
     1621 atatagataa attgtttgga attggaacat cttataaaga attgcattta caagagttaa
     1681 gtaacgactt aagaacaatt ttaggggaaa actgggattt aacaactaaa aagctaagcg
     1741 ttacactgaa tggaattttt attatagaaa agaaaaaata tagtaactgg catgatatag
     1801 aaactatgtt tttttataaa aatataaatc ctatatcatg ccagtttgaa aaaaaacgaa
     1861 tacaacttaa taatataaaa agatatataa ctgtagatga cataaaaaaa gatttagaat
     1921 tagacgaaaa agataagagt ttagaaaatg ctataaaata tacaaaagac aggatgttga
     1981 actctttaga tgaagatgaa aaactattgt tattaaaagg ttttatgtag agacaaaaat
     2041 aaatttcaaa aatatttaca taaggagata aaaaggatgt ttgaattaga tattaaagtt
     2101 aaaaataaat atatagtgag tgtgagttat gagttttcag agcttttaat acgagctttt
     2161 aatgactatg ataatttcat aaaagatata aaaaataaag ataaatttat acctataggc
     2221 attaatcttg gatacagaga attgttagaa gaaaattcgg catatcatga gtataatttt
     2281 tattatgatg aagaagttga taagtacttt tttgaacttc aatatcaaga acaggattta
     2341 agggaggagc tttcaaaaca acttttacta gaacttataa aaattaattt accagctggg
     2401 gcagaattaa ttgtagttga ttaaaattgt atttaatcaa aaagaaacag aatcaaaaga
     2461 acaattagaa atttgcatct aaaaacaaaa agtcagtcga cctggattta atatatatat
     2521 atatatatat atattccagg tcgactgact ttttgataat gagtcatcaa ttaaaataaa
     2581 cataattagc atacaatatt taaagaaatg ctataaaaac tagaaaacag aggttattga
     2641 gtaaattaga tgaaattgaa aaggcatttt taaacaatga atttgacata ggatttaaaa
     2701 ttgaaaaaga aatagagtta gtagagcaac tagaaatttg catctaagaa caaaaagtca
     2761 cgcaagtagg atttaatata tatatatata tatatatatt cctacttgcg tgactttttg
     2821 atgtgaaaat ataaaactta taccaaatgg aaaatcgcaa ggttaaaaaa attgaccctg
     2881 aactttccta atgtaagaaa attcaatgta ttttgaattg gaattatttt ccactagcaa
     2941 aatttgataa aaggtactat tattgtagta taaatgttta attgcgacaa aaaatcgtac
     3001 atgtgttctt gtaatatcga acaaaagggg gtataattaa aattgtaagt tattttttta
     3061 caaattttaa gaatgtatta ttctacttgc atataggggg atgatttggt tgaaagaaga
     3121 aataaaagaa atgttggaca aagtagggga taaggatata ttagagatta tttatctgta
     3181 tttaaagcaa aaaactaaaa aataaatatg tacatatgaa aagactatca aatttaagat
     3241 gatagccttt tattcttttt gaaataattc tagtagcttt tccattgcct ctaattcttt
     3301 gtcatttaac ttgctaaatt ttaacattac tttagcaaga cgttcattct tatctattgc
     3361 gcataagact tctgctgtta ttaagttatt gtttgaaaaa gtatacatat cgccttcgcc
     3421 tgtttttagc catttttcgt ttaccttaaa tgttgaagtt attaatttta aatcactttc
     3481 ttttattcgt gttcttccac tttcaagatt gtaaatttcg tgtctagtca ttcctagctt
     3541 gttcccaaat tcgatttgag atagcccctc tgcaattcgc aagtctctaa ttctagtatt
     3601 cattttaaaa ctcctttcat agcttaattt tacagtaaag ttgcccgtaa gtcaacgcaa
     3661 aaaaacgcat tggggcgtag tcttgcgaaa ataaattagt aaaagttatt gacttgcccc
     3721 ttattacgca ctataatata aatatattcg caataggagt caggaggttg tttctatgac
     3781 tagagaagaa agaattttaa gaaatgtaat tttgtactta gatatgagtg aggaaaagca
     3841 aagaatagtt gactgtattt taaatgcaaa gacaatagaa aacattgttg agaatgatat
     3901 tttagatgtt aataaactaa atgaagcagt aaaagaaata gaacaaatta tttgttaaaa
     3961 gcgtactttg acggctacgc taaaccaatt taaaaaaaga aaaatataaa aaggtggggt
     4021 aaaaaaatgg aaattaatag cgtaagagag ttaagagaat tgtttgaact ttttacagtt
     4081 ataaaagcgg aaggcaacaa tgttgtttct tgcaatgaaa ctgtaaacga gttaagtaac
     4141 aaagaactaa taaaagcaaa tgaaaacaaa tttaaaaaac cttggaatga tgctctatta
     4201 tatgaaataa atgctttaga tataataaaa aagcataagc acagtgatag aaaagaaaag
     4261 gcatataaaa cttatctagg attagaaaat gcagatatag aagaaattta taaaattcat
     4321 tatggagata aagaaaattc tgaaactgtt gcaagttgcg aagaaattag ttcagcaaaa
     4381 acggatacaa tatcttgcaa tcatgacagt gtttcaaaag gtaaagatac tagtgattca
     4441 gaatgtactt gttttatttg ttctaaaaaa atagaaaatc aagaagatgt atttaaaatg
     4501 ccttttttaa cacgagatta tttgagttca gcaaaagaaa taaaaaatat tgaaatttgt
     4561 gagaaatgtt tggatggtaa ttcgtggagt attataagaa gaaagcagtt taagaaaatg
     4621 aagaaataaa tatttaaatt agtatcttta catgtgaaaa aggagagtga aaaaatgata
     4681 aaaaataatg ttttaagtta tgaagaaaaa ataaaagtat taaaaaaaca tggttatagt
     4741 gattttacca aagaatatgt tttaaaagaa tttgacaagg aacatgcatt ttacaaatca
     4801 tttttaagag caatagaaaa agaagatgat ggaaaaggtg tgttaattaa aaaaattgga
     4861 aaaggtcagc atcaattttc aaaaggtttt ataaaaacac acactatcag tgagttattg
     4921 gaagaagcaa aacgtattag aaaaatatta gaataaggct tagaaatgtc tataatctaa
     4981 accttatact taaattaaaa tttacctaat atgtactcta ttgcgatagg tagggacata
     5041 tttaaaaaac ttgaagtgga actttttatt ttattccatt tagtgttatc tcgtatttta
     5101 tctaaatatt gataaccttt taaagacaac tgcatgacct ctaaaacagt atcatcaccg
     5161 taattaacat cgtcaacgag attattttga taaattaaat ttatgtgatg aacaatagtt
     5221 gacttatcgt atgtgagatt atctagtata gtatcaaatt gtaatacagg cgaaatggga
     5281 gttgtattag attcaataag taataaaata tctcttatac aatcttggtt agtaaccata
     5341 gtatcacctc cttttgcttt agtatagcac aggagaaatg agatgataat aaattttact
     5401 gtagcagaat gagaaggagc aaagcaaatg caaaagagaa ttacagaaat gagtatatta
     5461 gaaaaagttg atttaattgg acagaaacaa aagaaactac aagaaaaata taaaaatcta
     5521 agtaagggag aattgttttc tattgcttgt aatgaagtta aaaaaataga agagcaggag
     5581 ggaaaatatt ttgaatatag gtagttatac attagttaag aaacaactaa ttgaagaatt
     5641 gaaagaagaa attaagcaaa aagaagaaaa cttaaaagaa cgtgagaaat acataaaaga
     5701 attaaaagag aggttagaat gttttgcgag ggatgcgtta gaatcagaag agtgcataat
     5761 agatattgaa aatattttga tagaacaaac tgtagaatta gaagcttata atttattcat
     5821 agattgcatt ttaaacaaag atataagaaa agctattgcg atttataaca gaactaaaag
     5881 catcagaatt aagcataaaa atattaattc tatatctaag aaaattgaaa aaataaaaca
     5941 aataatttcc ttgtagaaat aggaggagtt taatgatgga taagtttgta acatgcgcta
     6001 gatgtgaaag aacaataaat gtagaagaaa ataatcatgt gaagtatgaa gaagaaactt
     6061 taaatttaag ttttacgttg tatttctgtt taggctgcgt agataaatta attgaacaat
     6121 ctaaaatgtt ggagggagaa aatgaataaa attagcgtta cacaagcatt agagaaattt
     6181 gacagtttac tagataattg gaacgacctt ccaaatcatg tttacacact tgaatataga
     6241 agcaaatttt acgaatggat aaagttgctt gaaagaaaag gtagtttaca aaattataag
     6301 atagttgaag ttttaaatgc tgaaagaaat ggagaagaag cgttgttttg gaattgaaga
     6361 aatataactt gaaaaggact taaacttttt ctagatagaa gggaggtata taaatatgaa
     6421 aatgataaat gtttttaaat tatcatcaat gtattgtatt tgccccaaat gcgggagcga
     6481 taaacttgga gaaggtgaag gaaagttaat tgttgatgaa catacttatt acagggaatg
     6541 taaatgtggt tttaatgttc ttgtagatga aagagaagac gaaatataag gaaggtgctg
     6601 gaaatgaata gtgttatttt aattggaaga ttaacaaaag acccagaact taaatatata
     6661 ccgggttctg gaacagctgt atcgactttt acaatagcag tagatagaga ttatattaaa
     6721 aaagatggaa ctaaagaaac tgattttata cctattgagg tgatggggaa gttagcagaa
     6781 gtatgtgcta ataatttagg taaaggtagg ttagtagcgg ttgaagggtc tataaaagtt
     6841 aatagttatg aaaaagacgg ggagaaaaga acttatacaa aagttcatgc taataagatt
     6901 aagtttttag actataaaaa agatgatact gaaaaagaat atatgtttga accgaaaggt
     6961 ttagaccaac aaggatttca agctatagat gacccaaata taccatttta aagtgggagg
     7021 gataacaatg tctaatacaa ataaaaaaac agctaaaatt tgcggatggt gtggaacaac
     7081 agtttattct tatatggatg gtaaaattgc atattgttct aagaaatgta agaaaaaaag
     7141 agaaaaaaat ttagaaacag aaggggcaaa aaaatgaaag agtatagaat tgaaattgga
     7201 gaaatgatat ttttaaaaca taaagttatt gttgaagttc cagaacaaat atgcgctttt
     7261 gatatatata aatatataga aaatgaatgt caagataaaa gtgaaatatc tgaatatata
     7321 aaaagtattg gtggcagaca attaaatttt atagaaggag atagcggaga cagcgagtta
     7381 atagttggaa gttgcaagaa aataaaaaaa ggagaaaaat aaaatgaaaa aaacaaaaat
     7441 cgaagaattt attactagag ttaaagaaag tatgcaaagc gtagcaaaag aaagtataaa
     7501 agacttagaa aatattttaa aagaaattga aaccggagaa acagaagtta aaattttatt
     7561 tattgacaag ggaaaaaaag ttgaagagat tgttgaattt acacatgacg aatacgtgaa
     7621 aatgattatt gaaaaaatga tatttgatag tcttaaaata ataaaagaac aacacgaaaa
     7681 gattttaaaa gagttcgaag aaagagaggt ctaaaaatga attttaaaat gattgcaaat
     7741 ataccaaagt ggacagtaaa agaacaattt gaaaaagtgc ttgaagaagt tttagaactt
     7801 aaagaagcta ttgaattaaa agataacaaa aaaataatgg aagaaggttt ggatgttatt
     7861 caagcaattt ttacactatt taaagtatta aagattgata aagaaatacg cgaaggtttg
     7921 aagttgcata ataaaaaact aagaaaaaga aagtggaaac tcgaaaaaat aaattagcag
     7981 aagggggagt taaaatgagt atatgtagaa agtgtttgta cggagactat ttagatgaac
     8041 tagtagtagg cgaatgtatt ttatgtggtc aagaattttt ttctataaaa actccggttg
     8101 ggaaaatgtg tcctagctgt tcagaaatgt ttaaggggaa ttgttctcaa tgtgcaaaac
     8161 caatcggaaa tgaaaaaaag tcatttaaaa ataaattaaa gtaaataggc aatataaaac
     8221 ataatttgat tgatttttaa attaaaatag ataatatact ttataaatat agattttatt
     8281 taaacttaaa aaaatgaagt cttatgacaa aaaaacaaaa taaaaatcca gaatattttg
     8341 aataatatgc agtagttgga aatgctacaa aatacctaac tagtcccaaa agctaaattt
     8401 aaggattagt taggtattta taacaaaaag tacaagaggt gtaaaaatga aaataaaaaa
     8461 agagtatttt gacaaagtgg aaaatgcatt aaaaatgtta ggagataaat atagaaagtt
     8521 aagagtatta aaaaaagaaa tagcaattct taaacacaaa gaaaactata gagaaattaa
     8581 ttatgaagaa ttagggttta aagtacaaaa aagtgtaaaa ggtatagatg acatggttgt
     8641 aacaatagag gatgctatct acaacaaaga aactgaaatt gaaattatag agcgaaagct
     8701 agagttttat aatatttatt taaaagagct atcagagata gaacaaaaaa ttatagaact
     8761 agtatacttt tatagctgga atgggaaaat gcctattaca aaaatggcga tggaattaaa
     8821 ttatgataga actagtcttt ataacaaaaa agctattgct attaataaaa tagctttaat
     8881 gatatatggt gatgaagctt tagaataaac attattttta cagaaaaaag actattttta
     8941 tacttcgttt ctaaagaaag catgttagaa ttgtatcata gaaaaaagtg tacattgaca
     9001 actgaatatc ccccgaaacc taaagtctaa acttgaagct cgaaagttta ggctttttta
     9061 ttgctaaaaa aggaaggggg tgagtaaatg aattttgtag aacctatcag aaatttagag
     9121 aagctagaaa gcatatgcgc ttatttagaa gaaactaaca tgagaaacaa tgtactcttc
     9181 agtatgggca tatatacagg acttagagta ggggacatat taaagcttag aataaaagat
     9241 gttcatgaca aacggtttat aactcttaaa gaaagtaaga cgggtaagac taagcctatc
     9301 gaggttaatc ctattttgag aagaatacta aaagaatata caaaagataa aaatcaaaat
     9361 gaatttttaa ttaagagtag agagcagacc aataaagcta taagcagagt catggcttat
     9421 aagataatac gagaagtagg cgaaatgttt ggtatagata atctaggcac acatacaatg
     9481 cgtaaaactt ttggttatca ttactataaa agaacacatg atgttgtaac attgcagaag
     9541 ctttttaatc atagttcagc tacgataacc ttgcggtata ttggaattga acaggatgat
     9601 ttaaacaaag catataggaa tattaaatac ttttagctta agagtttaac ataaaaaggt
     9661 cacgttaaac tggataaaat atagtctaat aataaagtct tgaatgtatt gttaatacta
     9721 aagtgtaagg ctgtttgata atacttaaat gagtttaaca gaatatatct tatgttaaac
     9781 tcaactagga aattaaagga gtatcactat gaataagtat ggtaagttta aatgtaataa
     9841 ttgcggaaag aaagttaatg taaataatag agatttaaag actaaagtta ttgataatac
     9901 tactgttaca tacttcatgt gtcgcagatg ctttgagaag tttgttgtat catgtgagga
     9961 tgattatatt aaacataaga gagaagaata taagaagctt aaagatgatg aagtcaagct
    10021 attgcaagac atgaaagagt atagcgacgg tctaggagag ctgtatatta atgagttata
    10081 agcacatgac actagataga ttaaaagaat taattttaat tgatagactt gatatattct
    10141 atagaagtac agactggcgt aaattaagaa aaaaagcact tgaaagagat aataatgaat
    10201 gtcagatttg taaagaaaaa aagaaagtat ctaaggcgga atgtgtacat catatcaaag
    10261 aggtaaaaga gtatccaggt gaagctttga agcttaataa cctaacaagc ttgtgcaacc
    10321 tttgtcacaa cgaaattcac gaaaaaatac taaaaattaa tcaagaaaaa aagaaaaaat
    10381 ttttcagtga agagcgatgg tagatagccc ccctattgaa aaaactggct ttcaaggcaa
    10441 aattttgtga accgaggtag gtacatggct ccgcaaggag acgtcaattt aacaaaaggt
    10501 gtggggggtg ggggtcttgc aaccaaaatt atggttgcgg gtgcagaaaa aaacataaaa
    10561 atgaggtggt gaaaatggat gataatgagg aattagaaat taggcgagac gtacagaatg
    10621 actacgtttt aggcatgact tacaaagaaa tgtcagccaa gtataacata cccgaaaaca
    10681 ctttgaaaag ttggagaaaa agagggggtt ggactcgcaa ctgcacccca aaaaagggtt
    10741 gcgtgaaaaa tgggttgcaa gagtcgggaa ataatttaaa aaactctata agaaaagatt
    10801 tattaagaca aataaaagag aataacatga cagaagaata cttcaaagac ttagttgaag
    10861 attatttaaa aatgtgggat ataaaaaatg aattaataga agatgtaaaa actagaggtg
    10921 ttcaagtcaa atataaaaat ggtcaaaatc aatggggcta taaaaaaaac gatagtgtat
    10981 cagagcttat taagtataac gctcaaatgt taagtttact tgataaacta ggtataaaag
    11041 cgacaccaaa agttttgggt gatgatgata ttgaattata agtacaataa atacatagat
    11101 gaatatttca acttagttga taaaggcgaa attgttctat gtaaagaaat ggaacttgca
    11161 gttaagaaaa taaaaaaaga tttaaaaaaa cctaatatct atatcgacca tgaaaaagtt
    11221 gaaaaagcta tcgaatacat ggaaaagtat ttctataaaa tgtatgcttg ggaaaaattt
    11281 gttgttgctt tagtagttgg ctgctttgaa ggcgacgaac ttttatatga tgaaattttt
    11341 atcatgatgg gcaggggaaa tggaaaaaat ggttttatat ctcaattagc taattatttg
    11401 caaacacact tacacggtgt aaaaaattat ggtattgata ttgttgcaat gtcagaagaa
    11461 caagcaaaaa caagttttga tgatgtttat aacatgttat ctgaacatcc tgctatatgc
    11521 aaaaagtttt ttaacagaac taaagaagta ataacattta aaaaaactaa cagctattta
    11581 aagttccgca caaataatgc taagtcaaag gatggattaa gacccggctg catcattttt
    11641 gatgaggttc atgcatacga aaattatgat aatataaaag tttttacctc tgcacttgga
    11701 aaagtaaaaa atcctagggt tttttacatt acaactgatg gaaatgtaag aggtggggta
    11761 ttagacgatt tcaaagagga atcaaaacaa attttaaatg aagaaattaa aaactctaaa
    11821 atgctgccac ttatttttaa gcttgatagt atagaagaaa tggaaaatat tgatttactg
    11881 gaaaaagcaa acccgagcat aaagtataat tcagacttaa ggacacaaat tttaaaagac
    11941 attgacaaag ctaaaaatag accgcagatg atgatagaat tactaactaa aagatttaat
    12001 ttgccagcgc aaaatttagc gactgttgtt gctgaatggg atgacatagt tgcaacaaac
    12061 gaagaattac cagacctaaa aggcttttcg tgcattggag gattagattt ttctagtata
    12121 agagatttta caagcgtggt attgttattt aaaaaagata aaaaacgata tatcatgcat
    12181 catactttta tatgttataa gtctttagaa ataacaaatt ttaaatttga tattgaatta
    12241 gcaaagcaaa aaggtctttg tacaataatt tatgatgaaa cgataaaagc agagtatatt
    12301 gctgattggt tcattgaaaa agcggaggat tataatatat taaaaatctg ctgtgactca
    12361 tatagagctg cacatgtaaa agaagaattt gctaacaaag gtttaccaat cgaagaggtt
    12421 agaaatggtt caataactca tgcgaaaata gcccctcttg tagaacaaat cttcgcagag
    12481 agaacaatta aatggggcga cgacatgatg atgcgttggt atactaataa tgtttatgta
    12541 gatacagaca aaaaaggaaa taaaactttt ttaaagatag agccaatcaa aaggaaaacg
    12601 gatggctttt ttgcattttt acatgcttta tcaaaagatg gtgaactgat gcaagaatca
    12661 acgattaagt tttatgattg ttatacatat taaaaataat gaggtggtga attgaatttt
    12721 aaaaatttta taactaattt tttatttaaa gataaaatgt ctggagcaga tggagagtat
    12781 tttaaaaaac tagaagctac gatattttat aaagaatttg cattaaggtc atgtatttct
    12841 attattgcaa atgctttagt tttaagtgaa tttcagacgt ttataaattc aaagtctgtt
    12901 aaggaaaaaa attattactt gtttaatata gaacctaatc aaaatcaaaa cgcaacagaa
    12961 ttttggcaag aagtaattac aagacttgtg tatgacaatg aatgtctagt tatacaagaa
    13021 aacgaacaac ttttcgtagc agaaagtttt aatgtagata agtatgtttt ttatgaagat
    13081 gtatacaaaa atattacaat tagaaactat aaattagata gaagctttag agagtcagac
    13141 gttttatatt ttaaattaaa tgttgaaagc ataaaaactg taatagatta tctttataat
    13201 gattataaag atttattaag tagcgccatg aagggttata aaaaaaataa tagcgaaaag
    13261 ggcgtacttg aaatagacac aaattaccca cagacaaaag aagcacaaga acgacttaat
    13321 gatttaatga gtaataagtt taaacgttat tttgaaagtg ataatgctgt tttaccgctt
    13381 tcgaatggat taaaatatac agaaaataca aaagcttcaa gtataaaaga tagcagagat
    13441 ataagagcga ttattgatga tgttatggac tttgtatgcg ctgcttttca tattcctgct
    13501 gggcttataa aaggtaatgt ttctggcgtt gaaggtataa cagataattt tttaaacttt
    13561 tccgtcaatc caatcgctcg acttataaca actgaaataa ctagaaaaat gtatggtaga
    13621 gataaatttt ttgaaaagag ttatgtaaaa gtagatacac aaaaaattgc taacatggga
    13681 cttgaaaaaa tatcaaaagc aagtgactta ctgtttagaa taggtgtaaa ttctataaat
    13741 gacaatttag aaatgcttgg tcgagaaaaa ataaatgagt cttgggctga tgaacattat
    13801 gtaactaaaa actatcagtc gattttaaat ccggatttga aaggaggggc taacaatgga
    13861 aatggagaaa agagcggtat cgctggaaat aagggacagt aatttagaaa gtcgtaagat
    13921 aagtggttat gctgctattt ttaatgatga ttatactaaa ttgcaagaca gatggggcga
    13981 tactttttac gaaaagattt caagaggtgc atttttaaaa actttagcag ataacactag
    14041 ggataagttt atgcttataa atcatgactg gaataaagtt ataggtagaa ctaactcaaa
    14101 tctaatttta gaagaagatg aacgtgggtt aaggtttgaa ctagatatac cagctacgac
    14161 ggacgggaat gatttgcttg aaaatgtaag acttggacta attaaagggt gttcttttgg
    14221 ttttaatatt gtaaattcaa aaactcgctt tgatgatgat tggacatatt acagagatat
    14281 aacagaagtt gaactatttg aaattacagc aacccctatt cctgcttata atgatactga
    14341 aataaattgt agaagtgata tatctataaa agaacttaga gaatctcaaa aagaaaaagt
    14401 aaaacaaaat gataataaag ataatataga aaaaaggaat gtcgacttag tatcggcttt
    14461 ttttaatgca tttaatcgag gaggaaaata aaaaatggca attaaaaatt tagatgaggc
    14521 aactaatgtt gaactaagaa cgaatttaat ggaagcgata aagagtaatg acgaagaaaa
    14581 aataacaaca tcatttttaa gaatggcgga agatatacaa agtaatttac taaaagaagc
    14641 acgttctatt gctaatatag aatcagctga cagggcgata cttgacaaga ggggtatgcc
    14701 acaattaaca agcgaggaaa gagcatatta tagccaagtg atagaaaaaa gaggatttac
    14761 tgatttagaa gtaactatgc cgcgtactat atttgatagg gtgtttgatg acttagaaca
    14821 aaaccacccc ctacttagtg caataacttt tgaaaatacg acaggggtta cggagtgggt
    14881 aataagaaaa acaggaacgg aggcggcttg gtggggacaa ttaactgacc ctattaaaaa
    14941 agagttagaa ggtggatttg caaaaattaa tactgttgct tataaattat cttcttatct
    15001 tccagttgca aaatctatgc ttgacctagg tgctgaatgg ttagacagat atgtaagagt
    15061 agttttgact gaatctatat cattggggct tgaaatggga atagtagcag gaacaggaaa
    15121 agaccaacct attgggatga tgaaggactt aaaaggctct gttgttgaag gtgtttatcc
    15181 agataaaact gcaaaaccat tagctgattt taaaccaact acattaggaa ctgaaattat
    15241 ggcaccgctt acaaatgatg gtaaaaggac agtcgcaagc gttataatgc ttgtaaaccc
    15301 ggttgattat tgggctaaaa tcttcggtgc aacgactatg ctaacttcaa atggcactta
    15361 tgtatatgga gttctgccta taccaggaaa tataattcag actgttgctg ttccccaaaa
    15421 taaattaata gtaggggttg ctaaagatta ttttatggga attgggtcag ctggtaaaat
    15481 agagtattca gacgaatata agttcttaga agatgtaaga gtctatcttg ctaagcagta
    15541 cgcaactgga acaccaaagg ataatttttc ttttttagta tttgatatag aaaacttaga
    15601 cacaacttta gcaacagaaa ttaattcaaa cacaaaaacg tcagctgcta gaacaaaata
    15661 ggtgaaatat atggaaaatg aaaacattaa aagtatagtt gaagaggtaa aagaatatct
    15721 taatgtaact tggatagaag aagatgcgaa aattgaaaaa atggttgtaa gaggaattga
    15781 ttactttgaa aatgaaatag caggaatagc actagatttt aaagataatt ttaatagaga
    15841 attgctattt aattattgca gatatgtaag aaacaatgct attgaatatt tcgaggaaaa
    15901 ttttagtaaa gagcttctta gacttcaaat taaaagtgca gctaatgagg taatatagat
    15961 atgaaaaaaa taaaatttaa taattttaat gatggtattg ttgagtttgg agaatacaag
    16021 gaaagttatg atatagaagg taatgcgtta gatgaaaaag agtttattgt aagtggtaaa
    16081 ctcttttatt ctaatgaata tataagagag caagacaaac tcaaatttga taatacaggg
    16141 ttaaaagtat ctattaagtt aaaagtacct tatatgagat taattaaaac aagtgatgtt
    16201 ataaagttaa ataatgaatt ttacagtata gtaaaacttg atattagtaa tgacaaaact
    16261 agtatatatt tatatttatc tgaattagtt gacgtactag ataagcatat ttctattttt
    16321 atcaaagaga gaaaaaatgt tcttgaagat gtagcaatga aattttataa aaaaatttgg
    16381 gcttgtgttg aagatttaaa tagtaaagaa ttgatagaaa ataattcgat taaaacttct
    16441 acaaataaaa agtttaaaat tagatatatt gaagaattag atttaagtat aaaacaaaat
    16501 gctataacta attttgttat taaatacaaa gataaatttt ataatataac tcaaatctta
    16561 aatttagaag aagaaaataa aatacttgaa ataacagcag aatctaagta aaaaaaagtt
    16621 aggagatagt atgcactcaa aaatagttac tatacttaaa aaattaaatc taggtattgg
    16681 ctttcaagaa tacgacaatt tttcaaatga tgaagaatat ataatatttt ttatagaaaa
    16741 tgaatatgat gcaaattttt ctgataatga aaataaaagt attgtatatt caataaaaat
    16801 agaatattgg tataagagct taaagaatat aaacaaatat attagtataa aagatatatt
    16861 taaaaaagaa aattttatct ttttaaattc tagtgatttt atagaaaatg aatattattg
    16921 taaagaatta aaattcaaat atgaggaagt tgtttaaatg ggtgtagaga taacaacaga
    16981 aggttttgac gctattttat ctaaaataga gagtatgggt aaatcgggcg ataaactgct
    17041 aaatgaagcc gtaaaagctg gcggtaatgt tattttgcaa gatgcattac caagggtcag
    17101 caaaagaagc ggaaagttaa aagatggttt aaaagtaagt ggagttaaga aaaaaggtgg
    17161 tactaagtat gttttagttg gtattacgaa agaggataat tcaaaaattt tttatggaaa
    17221 gtttttagaa tttggagcat ccgcgcatca aataccaatc aaaaaaggta agaaaaaagg
    17281 tagagtaata aatcatccag gcgttagtcc taaaccgttt ttagcacctg cgtacgagtc
    17341 aaaaaaagat gaagctaaaa atgtaatgaa agaaatatta aaaagaggat tggggttata
    17401 atgattaata taaataattt agtaatagaa acattaaaac ctttaaaaat acctgtttca
    17461 tttcaaaggt ataatggaga agaaaaaaca tatataactt tctttagtta tttagaacaa
    17521 aatttttatg caaatgatga agtggtaggc acagaacatt atatacaaat agatttgttt
    17581 agtaagaata aaatgagtta tataaataaa gaaattgaaa gactgcttaa aaaaaataat
    17641 tttataaaaa gaagtataca cgaaataaaa gaatctgatt atagttacca tactgttttt
    17701 agattcttat tttttgcgaa aaacgaggag gattaagaaa tggctattat aggattaaga
    17761 aactatagaa ttgtaaagct actaacagat acagaggaaa cacttgaata cgatactaaa
    17821 ataacaagat taacaggtgc taaaagtgtg aaaatatcac ctaaagttga ctcagcagaa
    17881 aattatggag acgaccagct tttagagact gcttcggcta tgggagcaat cgaagttgaa
    17941 atcgaagttg ctgatttgac attagaagag cgtgcgctta ttcttggata ccaatatcaa
    18001 gatggtgtgt tgatagagga taaaaatttc aatcctccaa accttgcttt tggttttgaa
    18061 tccccaaaga gccaaaacgg aagtagaatg gtttggctaa ctaaaggtgt ctgtgaacct
    18121 ttcgaagaag aagcaaaaac aaaagaggat aaaatcgaat ttcaatctca aaagattaaa
    18181 cttaaattta tgccgcgcat aaatgatggt agacacaaaa taacagcaga tacagatgtt
    18241 gaaaacgccc caacagatgt agagtttttc acaacagaat ttttaaaaac aggagcaaaa
    18301 ccaactgcac cagcaaaagc aaatttaaat aaataaaaag gagaaaataa aaaatggaaa
    18361 taaaattaac tataaatgaa gaagaaaaaa tatttaaagc tccacctata gcacttaaaa
    18421 aattagataa tgcctttgca ttatctgata aaattaatga tgggcttagt atgagagggt
    18481 tatttcaaga attattagat tttacaatag atatatatgg cgaacaattt acaaaagaag
    18541 aattgctaaa tgggttttat cctgctgacg aatttatgtc aaaagcatta gaagatttgt
    18601 caaaagtatc tggaaccttt gaagaaaaag taaaaaacta agtgatggag aaggtacaga
    18661 aaataattct aataaagaaa ataagtatct atctccacgg gactttataa aagatttata
    18721 tagtatgttt ttatttgaaa agaattggag catggtcgat atagacaata tggatatatt
    18781 ttattattta gatatactag cgtatatgaa gaaaaataaa agcgataaga ataatccgaa
    18841 tgaaaatgat gtctatatag accaattaag ctggttataa ggatgtgagt gtatgtgtaa
    18901 gtattgtgaa gtcgaagaaa gcgaaggagc ttttaaaaga ggtaaaacag acagagggga
    18961 attaataact tgtaattgtg atatagaaat gtggattgaa aaagaaggta atgagtattt
    19021 tttaaattct gaatgttggt gtggttacta tagtgaaagt tttgaaccta ttaacttttg
    19081 ccctttttgt ggaagaacat taatttaata attttgaaat aaaccaagat aaaatatctt
    19141 ggtttatttt tttgcaagtg aggtgaggaa atggcggaag atgtaggcga attagttgtt
    19201 cgtgttgcaa tggaaaacag ctcgtttcaa caagggatta ataacttgaa tagacaaatg
    19261 aagttaataa gaagtgagtt taaaaattct ggcgctggag tgaaaaattt cggaactagt
    19321 cttgatggtt tgaaatctaa acaacaaatg ttatctagta ctatacaaca gcaaactaaa
    19381 gtagttgaag catataaaaa taaactaaat gaaagtaaaa caactttaag caacacggca
    19441 caaaagcaag ttgaacttaa agaaaaagtt aatcaagcta agagtgctta cgaacagagc
    19501 aaagcgacat taggtgaaaa cgcagaagaa acaaaaaggc taaaatctga acttgatagt
    19561 ttagaacaag aatatgctaa aaatgaagaa aaaataaggt ctaactcggc agcggttgat
    19621 aattggtcta ttcgagttaa taatgcagag tcgagacttt ctgaaatgcg ttcagaacta
    19681 gatgacacaa cagaaagaat aaatcaacaa gaaaataaat ggaatcagtt agcaactaaa
    19741 atgactgaaa taggtaacaa atttagctcg gttggaaaac aaatgcaaga cgtaggtaaa
    19801 aaaatggcta tgagtattac tgctcctgtt ttagcagtcg gagcagcggc tagtaagctt
    19861 ggaatggatt ttgaagcatc tatgtcaaat gtacaaggat taagcggggc aacagctgac
    19921 gaaatggttc agcttgaaaa agcagctaga gaagcaggag catctacatc caaaacagct
    19981 aaagacgcgg ctgatgcgct tgggtatatg gctttggccg gctgggatgc gaaaacatca
    20041 atggaagctt tgatgcctgt tttgagattg tcggaagctg gaaatttaga ccttgcaaga
    20101 acctcggatt tagtaactga ctctatgtca agtctcgggc ttagtgtaaa agaattgcca
    20161 acatatttag accaagttgc caaaactgct gcaagttcaa acactaacat tgacgcactt
    20221 atggaagcta tgattgtgtc tggaggtact tttaaaaatt taaacgtacc tctttcggaa
    20281 gctaacgcac tacttggaat actcgcaaat agaggactaa aaggcagcga ggcaggaaat
    20341 tctttaaact ctatcatgat aaatctgaca agtggagcag gtcaagctgg tgtagctatg
    20401 aaagaactag gattaagtgc ttttgatagc aatggtaagt tcaaaggcat ggctaatgtt
    20461 ttgatggaac ttaaagaaaa aacaaaagac atgacagaag aacaacgaac aatgtatttg
    20521 tcaatgatag gtggaaaaac acaaatatct actttgcaag ctttgttatc tggagttgga
    20581 gaggaatatt cggaacttga aaaaaaggtt ggaaatagca caggcgcatt ggataaaatg
    20641 gctaagacta tgcaaaataa taataaaggt tctataactg cgttaatgtc ggctgtggag
    20701 gagttaggac ttaaaatata cgatgtacta aaaccaagca tagcgcaagc agttagttgg
    20761 tttcaaaatc ttacaaataa attaaacgaa atgaagccta gcacggtaga aactatagtt
    20821 aaaatagcag gattagcagc agcactcgga ccagttctat taattagtgg aaaaatagtt
    20881 agcatgatag gtggagttat aggggctttt ggtacttttt cggcagctat ggcagtcgta
    20941 acgacaggag cagccgcagc aactccagct gttggtgctt tagctgcaat tttaggtgca
    21001 ttatcaagcc ctgcggctat agctgtagct ggaattactg cgctaggcat atcggttgcg
    21061 acagtcgcta aagacatgtc aaaggatgct atagagccaa ttagccgttt tggtaaaagt
    21121 atatcagaat ctacaaaaga agctgtatct gcttttatgg atttagaaga aaagacaaca
    21181 gtatctttaa atcaattaat gtggagtggt gagactgttt cggagaaaat gaaacagaca
    21241 atcgtatcta attttacaga aatgtcaaat caaatcgttg ctaaacttca agagagcaaa
    21301 gaacaaggta ttcggtcttt gcaagaaatg tttgcaactt ctaaaaattt aagtgacaaa
    21361 gaaaaagaag agttaataaa aaatacagaa gaagcatatt caagcaaaga agaaaaaata
    21421 aaaaagagca atgaaaaagt aaatcaaata atgactaaag ctagtcaaga aaacagagca
    21481 ttaacaaaag aagaagcaac agagataaat caaataaaaa cagatatgct aaataccgcg
    21541 gttgacacaa tgagtaaaag cgaagcagag caagcggcga taatggaaag aatgaaagca
    21601 aatcatgttg atttatcagc taaagaagcg gcggaagtag ttaaaaattc aataaagaaa
    21661 aaagatgaaa gtataaaagc agctaacgaa gaatataacg aaagattaaa aatagctgcg
    21721 caacttagag cagagggtgg cgcagaaaat gaaaagttag cggataaagt agtagaagaa
    21781 gcaacaagac aaaaagatga atcaataaat aaagctaaag aaatgcatca aggagttgtt
    21841 gcagaagcac agaaacaagc agaaaaccaa gcgagtaaag tcgactggac aacaggagaa
    21901 ataaaatcta agtggcaagt tttaaatgat acggtaaaag gtaagatgca agaatgggac
    21961 gacatggcgg gcaactgggt agaaggcttg aaagaaaaaa tatctactgg atgggctaat
    22021 ttaaaggaaa atacagctat tttgtgggat aatataaagc ttactgtaac aactaaatgg
    22081 acagagataa aagagtctat agcctttaaa atagaagaaa taaaacaaag tattagcgaa
    22141 aaatgggagt ctataaaatt attctttaca acaacgtggg aaaatataaa aatgattttc
    22201 gggaacgctt gggatgctat aaaacaacaa gttgaagaaa aatttgcgcc ttttgttgaa
    22261 aggttaaaaa ttatatttga tggtataaaa gagtttttta gaagcacttg ggaagttata
    22321 aaaaatatag tacttggtgc tgtgttatta attttagatt tagtaacagg gaattttgat
    22381 aaactaaaaa ctgacgcaat acaaatttgg gaaaacataa aacaaagctt atcggatgcg
    22441 tgggaatcta taaaagaagt atttaacaat gcgttgactg caataagcga tttactttct
    22501 aacatgtggg aaggtatgaa aagtactgca tcaagcgtat gggaatctat aacacaaact
    22561 ttaagcagta tatgggaaaa tataaagtca acatgttcta atgcttggga agggctaaaa
    22621 acaatttgct caaatacttg gacaaatata aaaacaaccc ttgtaacctc atgggaaaac
    22681 atgaaaacag ctgttagtac taaattgact gaaattaaaa ataatattgt aactgtttgg
    22741 aatagtgtta ttactttttt taaaacactt ccagccaaat taaaacaagc cgctattgat
    22801 atgtttacaa gactaaagga aggtatagac tcgaaaaaac aagatgctgt taatgcatct
    22861 caaaaagtcg ggactgatgt tgtaaacaaa ataaaagagt tcccaagcaa gttcctgcaa
    22921 gttggtaaag attttgtaag cggcttagcg aatggtatat ccggctttgc ttataaagta
    22981 gtagacgaag ctaaaaaatt agggcaaaag gctgctgatg cggttagaaa agtgttagga
    23041 atacattctc catctcgtgt tatggctgag attggtaaat tcgttgatga aggtttagca
    23101 caagggattg ctaataatag cgactcggtc aaaaaagcaa ctgaaaagat aacaaagatt
    23161 atagaagaag aaacaaagaa agctacaaaa gcatgcgaag aagatataaa actatttaat
    23221 aaacaaatag acgaattatc taaacaagaa aaagcagcgc ttaaaaaagt aaaaggtgac
    23281 gctaaagacg cattgaaaga agaatatgca gctaaaaaag aaaaaataaa agaagcgata
    23341 gcactaagaa aagaacaaaa agaaaaagaa gttgaacagc taaaagagat agcaaattca
    23401 gctaaagaac agttaaataa agagttacaa gacagacaag agtttgtaaa aaaagttgat
    23461 gacttaacaa aagcaatagt tgatgcgctg aaagaaaaat acaaacaaga atacgaagca
    23521 cagcaagcga gtataaaaaa agagttagac gcactagata aatggaagga agaaagtata
    23581 gatagaatta atagtgtcta tgatgcgaaa attaaagcta ttgacaacga attagaagct
    23641 tttgaaaaag ctgaaaagga aaaagataga cttgaacaag acaaagaaga attaaacaaa
    23701 atagaagaaa ttaaaacagc tataaagttt gaacatgatg atactaacaa agaacagttg
    23761 caaaaagaac tagagaaggt tttagctgaa agaaaaaaaa gaatagagca acaagaaata
    23821 gaagataaaa aagaagcttt aaaaaagaga aaagaagaat tagaagaaaa gaaaaaagac
    23881 gaaatagaaa atataaatca aatctatgaa agcgaaaagg aatattataa taaaagactt
    23941 gaagacgcta aaaagttcta tgatgaaaga ataaaagaat caaaacttca agcagaagct
    24001 gaaaaattga taatggataa aaatcaaaaa gaaatagttg cgttattaaa tagttattca
    24061 gatgcatata aaaatgcagg tcaaactcta ggtgaaaaat tacttgaagg atttaagcct
    24121 gccataaacg aaattaaaaa tctaattgat agtattacaa gagaaataaa taatgctaga
    24181 aatgaagcat tgagtttaaa tagtgatatt agaaaaggaa attcagcagc taattcaaat
    24241 tcgggaagaa gagaagcaac ttataataat aattcaagaa cagcgaatta taatgtaaac
    24301 gttaatagta cagactcaaa cacagcaaga gaaatcgaaa gaacaataag aagtctagca
    24361 tttagtttat aaaaaaatag tttataaaaa aggggtgtga aacttgcaaa aattaatatg
    24421 tagaaactcg aaagggcaag aagttaaatt aggaaactca cgcccttttt tgttagaaaa
    24481 aatagaaaac ggagcaaata ctagtgctaa tatttcagtc tcgaacaatc taggtgatgg
    24541 tgtaagtata gatgatataa gcataaaaga gagattactt cctatttcag cagctattta
    24601 tgcaaaaaat aaagaagatt tagaattaaa acgtgcatat ttaacctctt tgtttaatcc
    24661 taaattagga gaaatggaat ttatatatac aaataacgca ttaactaaac gtgtcaaagg
    24721 agttgtgcaa gacataacat ttcaaacacc agttggattg atgcaaaagt ttcttataca
    24781 gctcttaact ccaagcccat tttggatgga tgagtttgtt ttaaaagaag aagtcgcctt
    24841 atgggttggt gacttcgaat ttactttaga aataagtaat gaaggaatag aaatgggtca
    24901 tagggtaagt aatttaattt gtaatgtagt gaataacgga gacgttgaat gtggcatgaa
    24961 aatacaattt aaagcacttg caacagttga taatcctagc ctatttaatg taaacacaag
    25021 agaatatatt aagataaaca gaacccttga agctggtgac ttattagaaa taacaacaga
    25081 atttgcaaat aaaagaatag agttagtaaa atcaaactat gaaagaataa atgtatttaa
    25141 ttggattgac ttggatagcg aatttttaca acttgaagtt ggagacaatt tatttagata
    25201 tgacgcagac gtaggaattg ataatttaga aatgtctata tattataatc cactttattt
    25261 gggggtgtaa tatgcagcag agcataagaa tactagataa aaatataaat ttaatagctg
    25321 aaatagataa ttacgaagaa ttacagatta ttaaaaaatt ccacaaagta ggagagttta
    25381 gtttaaaaat aaatgctaat aaaaatcatg tagataaatt agttaaaaat aatataattc
    25441 tacttggtaa aaattataat aaagtttgtt tgatacttca tcgagagttc atgtacaccg
    25501 aaacaggaga aaaaagcgac attttatctg ttaaaggcgt ggatttaaaa ggcttgttaa
    25561 atagaaggct catcataccc gacactggag aagctttcga aagtcacgaa gggacacaag
    25621 aagaaattat aaaagctttt gttaataata actgcgtaga ctcttcaaat ccaaaacgtg
    25681 taattgataa tcttatcatt tcagaaaaca aaaatcttgg aagtgctgat atgtggcgaa
    25741 gttcttacga aaatttaagt gataaaattc aagaaatatc agaattttgt gggcttggtt
    25801 gggaggtagt gttggatgct aataaaaaga aatttatttt tgacgtaatt actggtaaag
    25861 atttgactat taatcaaaca gaaaacccgc cggttatttt tagaagtgat tttaataaca
    25921 taagagctag acactttaca gaaagtataa ttaactctaa aaatgttgtg tatgctggag
    25981 caagagaaga cgcgacaaaa ctggtaattt caactggtga ggttgaaggt tttgagagaa
    26041 acgaagtctt tgcagatgta agtgaaaata ctataagcat actacaaaaa gaaggagaaa
    26101 ttaaattaaa agaatatgaa gagttaaaaa gttttgaatt agaaatagac cctaaaaata
    26161 cttttatgta tgaaaaagac tacaaactag gggatattgt tacagttcaa gatagaaaat
    26221 tgaaagtaac aatggatact agaattgtag aagtacaaga aagctatagt aaaaatggga
    26281 tgaaattaaa aattacgttt gggagcagca taccaacctt attttcaaaa ataaaaagga
    26341 tggtgaaata atggaaaaaa gttttgtatt taatagtgta aatggcgata ggagatacaa
    26401 ggcagaggac tttagagaat attttgctag ttttataggt aatggtgtat tccctaatcc
    26461 aagtagcaat ttgcaagttg tagataataa taataataat atgactataa cagtaaaaaa
    26521 aggcaagggc tggattaacg gagctatcta tataaacaca gacgatttag taattaatat
    26581 agaccccgca gatggaattt taaatagaat agacagagta gttttaagat ttgatacttt
    26641 aaatagaaat attaaattag ctgtaaaaaa aagtgctttt aatagttccc cagctgcaac
    26701 agaattacaa cgtgatgcag atgcgtacga acttggatta gctgatattt atatcagagc
    26761 tggagcaata agtattacac aaagttctat aacagattta agattagata aaaatttgtg
    26821 tggaattgta aaaggtacga tagaagaaat agacacaaca acattacttg cacaactaag
    26881 tacttggaaa gatgaagaaa taagtaattt caatgattgg cgggaaaatc aagagaataa
    26941 acaagaagat tggtataaca ctacaacaac aaattttgta tcagagttta acatttggtt
    27001 tgaaagtata aaagatattt tggatgagga tgcagctgga aacttattaa atgaaataaa
    27061 caaagtaaaa gaagagttag caaaaataga aacaacagca gagaaaacaa gttataataa
    27121 tgcaacaagt aatcttactg ctacaaatgt tcaaggggca atagatgaaa ttgttgcaaa
    27181 aatagaaaat tttaacgaga ttaatatatc catacaaaac gatatgttac ctatttaaga
    27241 gaggagagtg aaaaatggct atagtttatg aatttaatta tacaggtgct gaacaaagtg
    27301 tagtgttgcc acctggtaaa tataagtttg aatgttttgg tgcttgtggt ggtaattatt
    27361 atgattttgt acagtgcgca aaaggtgcct atactgctgg ttctttaata ttgaaagaaa
    27421 atactacatt acatgtttac gttgggcaaa gtggttattg taaaggtgtt aatggtattg
    27481 agacttgtag aagtggtttt aatggtgcgg gtggtataac cacttataaa agtacttctg
    27541 atggttatta tagtcttgca ggtggtggag ctactgatat tagacttatt ggtggtaact
    27601 gggataattt acaaagtttg ctatctcgca taattgttgc aggtggtggt ggtggtggaa
    27661 gcggaaattc acatgatagt attggtcatg gtggtggtac aaaaggtaaa gatggtattt
    27721 ctatagcaaa taaatatttt gcaggcggtg gaagccaatt tcaaggcggt ttaactttta
    27781 atagtcttta taatggttct tttggtgtaa gcggtgctgg agatggcatt agtggtgttg
    27841 gtggcggtgg tggttggtat tgtggtgcag gtagttttta tgctgaattt ggtggtggtg
    27901 gaagtggata tattctaaca aaagatagtt ataagccagc aaattatagt ccatcttcta
    27961 aatattattt tagcgatata aatagcgttg taggtggaaa tactacgaaa caagatggtt
    28021 atgctaaaat aacattacta caagcattgc catttttaac tatatcctca tataactcaa
    28081 ccacagcaac atttaaagct gaccacacag acccaacatt gttaactaaa atagaatatt
    28141 ttatagatga tgtactaaaa gaaactataa caacagattt aacccttgaa aaaacaatta
    28201 actatacatt agaagataat gcactacaca cacttaaaat agttgttaca gacagtgcta
    28261 atgctacagc agaaaaagtt gtaagtataa gcaaaggaat tgcacctctt cctgctggtt
    28321 ctacaacaga tgaagttaca agtaaatgga tagaaattaa agatgcattt aaaagtggca
    28381 aaacaagtat tataaacact ttagcattaa aaaatataga agcaagttta aacaatacat
    28441 tagtagagtt atcagagaaa attaaaactt cttttgatag ttcggacgct agtgtgcagg
    28501 atttgatgaa ccagctaaca caagctaata atactataag tcagttaaat acaagatata
    28561 aagttgcgag tggaattact tatcaactca ataacccaag tttatctgcc aatttttata
    28621 atggtggtta tacaactact caagaccatt ggattaatgt tagcaatctt ggttttgttc
    28681 ctcatatttt tatagctgag tgcgatttta caaaagatgg ttatttaact aagagtttag
    28741 tttttgccag ttataatgtt tttagtaaag attatgtaat ttcttcatat tttagacgtc
    28801 aaacaaattc aactttttac tcacatggca acatttataa tcttaatgaa aaagatgttt
    28861 atgttaatgg cagaggtgtt caacttcctg cttttaataa ttacgatttt gcatataagt
    28921 ggcaagctat aaaatttgta taaatggggt gataaaatga atagagcaaa tagaataatt
    28981 tacgaccaaa caggcaaaat gcttctccaa acaggagaag caacagggga tgtattggag
    29041 tatgatacaa taacagaatt gcattgcatt gatattccgt atggaagtat agattatact
    29101 aaaaatagaa ttataggtat aaatatagaa acaaaagaac caattttaga ggaaatacca
    29161 acatttattt cagatgaaga aaagagaata caagagttag aaaatcaatt actaattgca
    29221 gaaaatgaaa aggtaggagg aattttataa tgaatataaa taatgttgta gtaagaatat
    29281 tagcagagag aatattaaac ggaggcttaa accctttaaa aaatcgagaa tttgaactgg
    29341 atgatgtaac taatgcagaa tacagaaaag cagtagagga ttatattata gagcatagtg
    29401 gagtagtaga aggaacacaa cctacaaaat aggttctttt tttataggct tagataattc
    29461 taagtcttat tttaatgcaa aataaggagg aaatatgaac gtaacaatag tttttttagc
    29521 aacaaatata tttataaaat tagtaatatt agcaatagca tttgatacat tgctaggttg
    29581 tttaagagca ataaaaacac atcagtttaa ttcaagtttt ggaataaatg gaggaataag
    29641 aaaagttgca atgatagcat gtatattttt tctagcagta gttgacattc ttacaaagtt
    29701 taacttttta tttatgttac cacaagattg ggttgatttt ttgcgattaa atcatcttgg
    29761 aatatctgaa tttttctcta ttttatttat tctatatgaa agtgtaagta tattaaaaaa
    29821 catgtatttg tgtggattac cagtgcctaa acgattaaaa gaaaaaatcg gtagtttatt
    29881 agatactatg acagatgaat taaatgttaa aggaggtagt aaataatgaa aatatgtata
    29941 acagtaggac acagcatttt aaaaagcggg gcatgtactt ctgctaatgg agtagttaac
    30001 gagtatcaat acaataaatc tcttgcacca gtattagcag atacttttag aaaagaaggt
    30061 cataaggcag atgtaataat atgccctgaa aagcagttta aaactaaagc agaagaaaag
    30121 atttataaaa tacctagagt taatagtgga ggatatgact tacttataga gttgcattta
    30181 aattcaagtg gtgtaggagc ctttggaaca gaagtatttt actatagtga aaaagggaag
    30241 gaatatgcgc aaagggtagt agataaatta tctaaacctt tcataagaaa aaaaggagat
    30301 aaagaagtag gtaatagagg tgttaaatta gataaaagtt tatatatttt aaatagttct
    30361 aaacctactg caatactaat tgaaagtttc ttttgcgata acaaagaaga ttatgagaaa
    30421 gctaagaaac taggtcatga aggtattgct aagttgattg tagaaggtgt attaaataaa
    30481 aatataaata atgagggagt taaacagatg tacaaacata caatcattta tgatggagaa
    30541 gttgacaaaa tccctgcaac tgtagttggt tggggctata atgatggaaa aatactgata
    30601 tgtgatataa aagattatgt accaggtcag acacaaaatc tttatgttgt tgggggtgga
    30661 gcatgtgaaa agataggttc tattactaaa gaaaaataca caatgataaa aggaaatgat
    30721 agatttgata cactttataa agcattagat tttattgata gatagattaa gaaggtagca
    30781 actaatttgc tagttgctac cctctttttt tatgcctact tttctttctt tttccaaaca
    30841 acttcgtacc ctattagatt agcaatagtc aagatttcgc tatatttcat agtctcatta
    30901 tttatttttt gcctcaaatt ttcaactgta ttattcttat tattatgttc attcattaaa
    30961 ttatttaact cattcatgct gatattttct ttcacaagaa tacttttgat ttcatttttc
    31021 catgtcattt tttcacacct ctcttattta tataataaca taaaaaaatt aaaagtaaaa
    31081 gaataatcta tgttaatcaa agttatattt tcggtaatca aagaaaaaac tttgaaaaag
    31141 tgttgactta gatattcata tagattataa tataattaac aaataaaaca agggggtaag
    31201 caaagaatga aaactttatt taaagaagcg cataaaatag caagagaaat taaagaaaaa
    31261 tataatgatg tagattataa agtacaattt agtctttgct tatccttctt aaataaaaag
    31321 ggggatataa aaatgaaaga attaaaaggg tcagaaaaac aaattatttg ggctaatgat
    31381 ataagagcag acattttaaa tttaactaac gaactagaaa aaagtaaact agaaagaata
    31441 aaaaatgaag actacaaaat caaagacatg agtatagaag aaatgataga aagatgtaaa
    31501 agaaaatttg aaagaataag agaagcaata agcaacatag aggatgctaa gttctttata
    31561 gataatttta gaaatgtttt aaaatataat tctttaaatc aaaaagcgtt tcaaataaac
    31621 cagatttttc gagagtctca atttacagaa gaaattggaa atttaaaatt tttaaaagga
    31681 gaaactcaag cgtctcataa acttagaaaa gttgaaggta gtataagcta tgaagaagct
    31741 aaaaaaatag ctaaagaaat aaattttaat gataattggt ctaatgaaat aaaagaaaat
    31801 gtacttgaaa tagttgaaat atttgacgac gcagtaaaac aagctactag cacagaatat
    31861 atagagaaag ctaaaaaata cataatagaa acattaatac aaaaaaatgc acaattttat
    31921 aaaaatggat ttgcagacat taaaaagaag cagattgaaa actatagaaa aatagaaagt
    31981 gctgaaagaa aaatagaaat tgcataccaa atatttagag atggtgtaga gtgttaaaaa
    32041 ttttggattg gtacaaatat agttcaccgt cgaatgctat aattttaata tataaaataa
    32101 atggaggatt gaagtatgaa tatagatttt agtacaatat taaatttaaa agttaataaa
    32161 gagaatgttg aaatgataga aaaagagatt aataaaatca acaatgatat atttgatata
    32221 agtcttaaag attgtgcaaa cagtcacgtg ggaatcgtta aagaaacagt tggaaaaagt
    32281 ttaaacattg aaacgtgggg gcatggtgaa attaacagta gcataggaat aagattgaga
    32341 tttaattttt attattcaaa aggcgacaaa gtagacatag aaaatataga aaacatttta
    32401 atatatgtaa atagcttatg cagcaaaaat gtagaaagtg accttatagc tagtgtaaga
    32461 cctggatttt ataaattaga agagaaatat tctacacgcg ttgaaaaatg cgaaattaga
    32521 cacgaattta caggcataaa caaacaagaa attaaagaat ttttagagta tcttacagaa
    32581 attgaaaaga gcgaaagaac tgttatgtat aaaaacaact aaaaaatgga ggatataaaa
    32641 aatgaataaa caaaaagcta gaagattttt aagagttata gatatgaata tagataaaat
    32701 agaggaagaa gctataaaag cttttaaaga aagttgttta atcaaagaga ctaataatat
    32761 aaaaatttat atcgatatac aaggaaaagt tgaagcgata gcagttcaaa cttgggctaa
    32821 acttttaggt gatgacaaag aaattaatat tttcacatta aatcaagcgc caactcattt
    32881 aaacgatatg cttggagaaa tttgttacgt aaacgattat gaagaatttg aaaattggtg
    32941 tgaaaatgag tgggaaaatt tggattggga tagttataaa aaattcaata aagaaaattt
    33001 cgaagaaatt gctgaaagaa atatagacga tagcacatca gtttttttag aagaattaca
    33061 aaaaggcatt gaaagttgta aacaagaatt gcaaaatgta attgaaaatt aagtagaaga
    33121 gtattattat taatgtatat agtatcgctt ttaagattaa ctacgaggga tttaaaatat
    33181 tttttaattt atttagaaat aatattgtaa taagaatttt aagattaact atgagggatt
    33241 taaatgtcaa tttaatttat atagaaataa tataatatcc aattttagat taactatgcg
    33301 gaaataaaaa cagtgtattc aataaataca ctgtttttta atgtttagtt attatgataa
    33361 cttaatcctt cgattataaa tcctacattc gttgtcatct ctaattcgtt acttaacatt
    33421 attccttgtg taaattcatt gtcagctggc aatgttactc tttttctagc aattgcttct
    33481 ataattgttt gaaatagttt aattttcata tcattatcat gtccagcgaa tcgtagataa
    33541 ttcgttactt cttgcgaaaa cttattcaac tccgatttag tcattttttc agataactct
    33601 tttccaattt gcagataaac gttcatcttt tccaacccct ttttagatta tagttaaaac
    33661 atattattta aacaatatta atatattgta ataataatat taaaatatta cttaaatatt
    33721 atatatacaa tatttaagta atatctaaac aatataaata taatattatt tactttttct
    33781 tagtcttatt tcttctattt ctctcattgt atatatttaa attctcttct tttatagtga
    33841 catcagataa aatttgagtt agtatatcac ttaataattt actaacgtcg ctgttcatat
    33901 caatagaaaa agattttaat ttgtttacaa gctcctcatc aagcgttgca ctcatagtca
    33961 cttttttact tttcttatta actagattat atatgtttaa tgtcttattg tctgtagttg
    34021 tagaatcatt ttgtacgatt gttccatttt tcaaaatcgg tttttcttta tcaactaaaa
    34081 ggtctctgtt gaaacttggc atctataaca ctccttttct tttcaattct tctaacatat
    34141 tagtaaattc ctgtctgctg aaatgctctt tagttatttt tgtataatct gttagagata
    34201 gtttttttaa tagtgccttc tctataaact cattttttct aatgtatgta tctaacataa
    34261 tatctctcat atcgttaaac ccttccagat aagaatcgaa agtatcgcta atttgagttt
    34321 tcttcttctc aaatccaact aaaatagtac tttttatatt atcctcctta tcaaaatagg
    34381 ttctatcaac atcccagagt tgtttaaata attctgctcc tcttaaagaa gaaatgtttt
    34441 tatcttgaat tggaataatt atactatctg ctaagaaaag cacattttta gctgtcaaat
    34501 cgtagcgtgg agataaatca cagataatat aatcatactc actcaaagta ttaaagtttt
    34561 gcatgtacca gcgagctaaa aatttttctc tggctggtaa aggtgatagc tcttgttcaa
    34621 atctagccat ttgtatgtca gaaggaatta agtctacatt aatgtaattt tcattaggtg
    34681 acttgactat tgcatcatta gcactaatcc cttttaaaat atctaatgta gtattatcat
    34741 tatgatttat ttcataaaca tattgtgtta gatttgcttg cacatctaag tcccataaga
    34801 gtattttttt atctttatct ttcgcaagtt catacgctgt cattatagac gttgttgtct
    34861 tatagatacc tccttttatg ttaaagtacg taagtagctt tgtattagcc attttctcca
    34921 cctcattatt atatttataa tattataata atattgtagt attgttttta taatattgca
    34981 acaatatatt aatattgtta attacaatat tcaaataata taaatacaat atttacatat
    35041 tgcttttaca ataccaatat attatattaa aaatactaaa atattgtata tacaatacta
    35101 aaataatatt tttataatat tacagtaata tttttatatt atagtaaaat ttaaaaaaaa
    35161 gaaggctaag aaaaaaagca accttctttt atagaaaaag tcattcattc ttagcctttt
    35221 caattattat cctgtcgccc tcgaatgttg ccgtaacatt agggttttct cttgttatgc
    35281 ccatttcatc agcccatttt ttaggaacac tcattctagg agtaaacgac ccactcccac
    35341 ctttgtgaaa gttaaacttt aaatctcttt tttctttttc cattcattag ctcctttttt
    35401 gctgtattac aaagaaaagc aataatacaa cactaaaaac taaaccgagc atgttgcttg
    35461 tactagagtt taatactata gatactatat ttaagactat agttatcaaa actaaaacaa
    35521 tgcaaatctt ataaaattta tcactcatgt ttttttagaa atgtgttata atattagaaa
    35581 gaagtcacct agagagaagg gctattccct ctaggcttat ccttacagtt ctttcaaaat
    35641 tcttagaact gcgaggatta tgttaagtaa taataggatg aaggagaaaa cttttttaac
    35701 tatgttaaat ttgttttctc ttttcttttt acttctttct aatctcccca ttcccgttcc
    35761 tcctttcttt tatttttttg ctttctcacc tccttctcta taaatatatt ataccatgac
    35821 gggtcgtcaa aaacaatagt tttctcctat tttttaaaca aattttggta aaaagtaaag
    35881 attttagtat attagaagtg atgtaaaatt gtatcttttt ttaaaaaatg tgatataata
    35941 aaagcaagga aataatgatt tactttacac aagagtagct atttccaatg ttttgaaaaa
    36001 taacttattt tcttaaacca ctcttattgg cgtttgagtg gttttttaat tttatcgttc
    36061 ttaaaataaa ctttgataaa aataaaaaaa atagaatact gaaaatgatg taaagttgta
    36121 tcttttaaag aaaatgtgat ataataaaag caagagaact gtattctagt cataagagta
    36181 gagttttagt ctaaaataaa aaattattta aactttttga atttgattct caaatcaaat
    36241 tcccaaccac tcttttgcgc agagtggttt tttacttgtc taaataattt gctaattata
    36301 tagaatatta aactagctat aacaccagct aatacattaa gtaaaaaatt atccatactt
    36361 catcacctcc ctttgtagcg ttgggaggat aatcttttgt gtatagactc cactcttttt
    36421 ttagaatata gattgctttc tcttgctaaa attattataa catatattta cataaaaaaa
    36481 gacttagttt atatactaag tcttttaaaa tgcagtattt tagaaaatat atttttaaaa
    36541 tataatttat tttcaaaaag agtatatttt tttataattt aaatgtgcta taattataat
    36601 cacaacgaac agaaaacttt tttttaattg gtttaaaaaa atctacaaaa aaataaacaa
    36661 ggaacatgta cttgccgtca aacaaaccat gttccttaca ccggaggtta gacctctctt
    36721 aaggataata tatccttgtt tttctatact tatatttata ttataagaca aagtgtagaa
    36781 aaacacaatg gttttaaggt aattttaata gtaaaattat tacaaaaaat caaaaaaaga
    36841 gcgtcagcct ccaggtggaa attggaggga taaagatgaa gaatgttaat aaaaataata
    36901 tagataagat gtttttagaa gaatgtagcg ctgaaagagc agagcatgaa aaagaaaatt
    36961 attcaaattt caatagacat gaaacatatg aaataaaaaa atatataaac g
//
LOCUS       DAYAPA010000027        36654 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.027, whole genome shotgun sequence.
ACCESSION   DAYAPA010000027 DAYAPA010000000
VERSION     DAYAPA010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 36654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36654
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.027"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            293..670
                     /locus_tag="ACGI59_001255"
     CDS             293..670
                     /locus_tag="ACGI59_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HFG9739058.1"
                     /translation="MMIRKIPQAELKVMKFIWKVDVTVTSKDVIEAMEQKYGWKQTTT
                     LTLLSRLVKRGFLDAQKIDRYTHYTIRVKHKDYLSFETKDFLSNIHDDSLRSLISALH
                     DDENIGKDKLDFLESYFDNLKDE"
     gene            663..1811
                     /locus_tag="ACGI59_001256"
     CDS             663..1811
                     /locus_tag="ACGI59_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HFG9739059.1"
                     /translation="MNNYMLYVFENLIKTTIVCSLGICLLLFLKRYLFKKFSKRFNYY
                     IWLIIVFRMLLFLFNYTIVYEVKEPKENALGNNITQIDISTDNNLMLYVAYLWLFVTI
                     VIAVYTFIKYTRFKNLVVDVSYDIEDNDINCIYKNLLKELNIKKKIELRGSDELISPA
                     GMGLFKSYIFLPDYPYSKDELTWILKHELMHFKNKDILIKFLVLSVRIIYWFNPLVYV
                     MSNKVNLDCELCCDESVLVECSLKDKKEYALALIKSIKFSKNYNSKILTTEFDKTNLE
                     KRLESIVKKKGKSGILIAVLVFMTSITSFVEVDAQVRIDEGNNGMNNNINEFLLNKTT
                     DKIINIYYKDAPNEIRKFYEDKCELEGKIPRDYDIIEMDMKDYNQLIK"
     gene            1828..2430
                     /locus_tag="ACGI59_001257"
     CDS             1828..2430
                     /locus_tag="ACGI59_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HFG9739060.1"
                     /translation="MLTKLRKIVAIMCCLLLTFTSVAFADTSKEKGGSKETVPEKNLD
                     SFTLSGYIYTYENAPQEIRDEHKQNCEEINIDPKPDDEIFVPESANLMNEDNSKGVYA
                     SYTVSYNIGAKTITIMSNEYLTISTTKVIRKGNSGKEVKAAQIMLTLLGYNVGIDSSF
                     GSKTYNAVVSFQKKYGLSADGIIGPATWDKLGRLTDPTLS"
     gene            complement(2523..3545)
                     /locus_tag="ACGI59_001258"
     CDS             complement(2523..3545)
                     /locus_tag="ACGI59_001258"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HFG9739061.1"
                     /translation="MDYNDLLKSARENFNGTCKVCKICNGLACAGDVPGMGGKGSGSS
                     FIENRKSLEKIKINMRVIHNVSKPDTSIELFGRKMSSPIFAAPVSGTILNMGGKVSEK
                     EYIEPVVRGCSNSGIYAMVGDTNVDTFLLDNLDVLKDNRGNGIVFIKPWNNSKIIDKI
                     RLSEEAGAFAVGVDLDACGLINNQFQENPFSPKTIDEIRELVESTRLPFIIKGIMTVD
                     DALMAVESGASAIIVSNHGGRVLDYTPGTCEVLPDIAKSVKGKITILVDGGVRTGVDV
                     VKMLGLGADAVLMGRPFVTASFGGGLDGVEFFIEKVRNELCETMILTGCQNVKDIDGR
                     VIWNLL"
     gene            complement(3874..4056)
                     /locus_tag="ACGI59_001259"
     CDS             complement(3874..4056)
                     /locus_tag="ACGI59_001259"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9739062.1"
                     /translation="MYKDKSDECIHLMTAYIDSISGYYSFIDTQLEDFMMKYGENIVD
                     SNLHSIMMLLCKWGLS"
     gene            4369..4788
                     /locus_tag="ACGI59_001260"
     CDS             4369..4788
                     /locus_tag="ACGI59_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3785 domain-containing protein"
                     /protein_id="HFG9739063.1"
                     /translation="MSLYKFIYDDKEYLLKEENCSALINDEENPVQGVSISKIIDILN
                     EAEEVDFDVEYYQEACPLCLEGVKEKKKFFPFLEYHFYIFSKDGEYVISNISVDYKGL
                     SFNKLSRANKVDNSYIVSVIICENCQDYIVQIENCIV"
     gene            4919..6760
                     /locus_tag="ACGI59_001261"
     CDS             4919..6760
                     /locus_tag="ACGI59_001261"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFG9739064.1"
                     /translation="MEEIDDTILDSMFKLTLNERLLTNNDFIENKIDVVKNLIDCYKT
                     EQNSLMLLKSNFLLALLYGIQGFSEKMKEYILISCKYIDKCLPKDYKFLARFYSQIAI
                     LSLKNEDVNKANLYMDKFNLICDENNFLIEEIIFKSQLIYIKASKCIESSVIIKEIET
                     LYIKIKEINDFNCKKIYFFIIGKIYFLFLDDALIAKVNFLKARKYADLLKDMQVSSLC
                     NIKIGECECSFENYECSMEYFNEVISNKKYRNVNIIQKYRASNNITKILIKTRNYPVA
                     INNLAKSEVYLEKIKNHSLKEVEKFDLFISLAMYYAKSNEKSFEQSICYLDRAKNILN
                     MIKNIEEYIIYDLEIIYHQAKIYYIFGYYDNALNTNKKLLEKSKNAKNYNYVKLAYKS
                     IYLCFEKIQNYEMSMKYFKMYYELKMMYIKARNRNYIDSLNYRHEYIEKEINNMRKVK
                     LDLDKKKYVDHLTSAYNRTFLNDFLEKQEVSEYCTAFMIDVDYFKEYNDTYGHYNGDI
                     ALKNITIIIKKYLKKDMLMIRYGGEEFLILSVCKDYKKSKIFGRKLCRIVKKFLNNNL
                     TVSIGIDTCKNSSIDIKEIIENADKALYKAKESGRNRCLHYHDFKNF"
     gene            6844..8674
                     /locus_tag="ACGI59_001262"
                     /pseudo
     CDS             6844..8674
                     /locus_tag="ACGI59_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454680.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            8682..10682
                     /locus_tag="ACGI59_001263"
     CDS             8682..10682
                     /locus_tag="ACGI59_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFG9739065.1"
                     /translation="MILLRKKIGIIASDIELKERIEELYREDVENGTIIIDILNLDLM
                     ENQGRILVEKGAQAIIGRGGGYSLVIDTVNVPVIPMNMKSTDLLRAIEIAKKYSKKVV
                     LILGDNEVSFDYVGWRNVISTEITEEWFESKYEIRSKVVKYIDQKDEVVIVGGGLACS
                     FARQYGIDSVFATASDESIREAVEYCKKLLDTLGEEKFNNEVLRNILDGIKDGVIAID
                     SNGSIILYNESAKNMLKVERKCALNKYILDVFPKMEWMLDCLHEKEDVEDRKIRNINN
                     LIVNTRTTLIKVDNSTYGVLGIIQDITKLQNLERKIRFDLNQKGLYARYTFDDFLFKD
                     KLTKEFIEEAKKIGKSDYTTLLYGESGSGKEIIAHSIHNISKRKDRPFVAINCATIAE
                     NLLESELFGYEEGAFTGARKGGKRGLFELAHGGTLFLDEINSLSFNIQTKLLRVIEER
                     QIMRIGSDYIIPLDIRIIAATNESLTEKIVMGTFRADLFYRLSSLEINIPPLRDRRED
                     IIPLFNNFVNEVLKDDGLNGINSIDENFVLTKDEMDKLYNYSWPGNVRELKTIAQKYV
                     VTGKIKLRQDRDFKTKKLLPNSEVDKFNSETTASVEVQDESINISKINDGKISIDIKE
                     VNKYVEEKIISMLFAQGLSKNEVAQVLGISRTSLWKKYNKNI"
     gene            10944..12203
                     /locus_tag="ACGI59_001264"
     CDS             10944..12203
                     /locus_tag="ACGI59_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFG9739066.1"
                     /translation="MSQSMVGKHAMWPKENDVIFSISGRAQAAEKAFGMDNVINATIG
                     ALMDDSGKLITMKTVYEEYKALDNCEIGAYAALEGQPDYLEAVKKVFFRDYLPEGHIR
                     VLASPGGSGAIKLAVWNYTNEGDEVLTSDWFWSPYVSIAEEANRKVVNYQLFDENRRF
                     NFESFKEKFVNIAEKQGRVFTIINTPAHNPTGYSVADDEWDKILDLSKEVAKDKDKKI
                     IFFVDSAYIDFAGDDDVCRKFFKKFSNLPENVLVLVGFSMSKGFTAYGMRMGAIICIS
                     SSEDVAEEFHYSCVHSCRANWSNCNRSAMAVLSNIVNDPKKFKEYEDEKEIYKNMLTR
                     RADVFVKEAERVGLEILPYIAGFFVSIPCDNPKEVCEELTKHNLFAVPLKMGLRFAVC
                     AVSEDKCKKAPSIIKEALESLEVKINN"
     gene            12264..14051
                     /gene="iorA"
                     /locus_tag="ACGI59_001265"
     CDS             12264..14051
                     /gene="iorA"
                     /locus_tag="ACGI59_001265"
                     /EC_number="1.2.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043805 - indolepyruvate ferredoxin
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HFG9739067.1"
                     /translation="MKQLMTGNEAIARGAYEAGVKYASAYPGTPSTEILENIATYKDA
                     IVAEWAPNEKVALEAAIGGSIAGARTMASMKHVGLNVAADPIFTYAYTGVNGGMVLIT
                     ADEPGMHSSQNEQDNRMYAKFAKIPLFEPSTSQEAKDMIKEAFEVSEKYDTPVLYRVT
                     TRLCHSKGLVECYDREEVEIKEYVKNAKKMVTVPANAQIRRGVVEERMEILKKFSNET
                     DLNYYEINDTKIGVIASGMCCNFAKEVFGKNASYMKLGFTNPLPYEKIKEFAEKVDKI
                     YVIEENDPFIEEQIKAYGIDCIGKDVIPPYGEMTPDVLRKAIFGKTNDTIEYKSELVT
                     PRPPSFCAGCPHRGFFYELGKRKNLIVGGDIGCYTLGFAPPYNGIDYVVCMGSAFGTA
                     HGAQKVLNMKDDNEKRLVGVLGDSTFFHTGINGLLDVVYNRGNSISVILDNRITGMTG
                     HQENPGSGYTLQGAKTKEVDIEGLVKACGIEHVRVINPNNLKEVNEALDWALAIEDEP
                     SVIITRWPCVLKKFSKEDIEEFNNPFKTKCKVDHDKCIGCKLCLKTGCPALSFDKENK
                     LSNIDRNQCVGCGVCAQVCPKQAIVKEEK"
     gene            14053..14628
                     /locus_tag="ACGI59_001266"
     CDS             14053..14628
                     /locus_tag="ACGI59_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HFG9739068.1"
                     /translation="MTKSILLVGVGGQGTILASKLLTMGLMEAGYDVKMSEIHGMSQR
                     GGSVSSQVRYGDCVHSPVIEIGGADILVSFEKMEALRWFNYLKPEGKALVNNHRIDSM
                     TVLIGGAEYQESDIDAELSRLNAKVINAADKAEELGNSKIMNVILLGCLVKSMQLESI
                     DWEKIISENVKPKFVELNIKAFHEGMEMVSK"
     gene            14681..15751
                     /gene="buk"
                     /locus_tag="ACGI59_001267"
     CDS             14681..15751
                     /gene="buk"
                     /locus_tag="ACGI59_001267"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HFG9739069.1"
                     /translation="MTYRILAINPGSTSTKIAVYDGEEQILVKTIDHPAEEIAKYNTI
                     QDQFEMRKEAVLNILKENSIDLKSLSAIVGRGGVLPPVKSGAYLVNEEMIDVLRHRPV
                     LEHASNLGAVVAHAISEPLGINSYIYDSVAVDELIDVARISGLCGMDRSSAGHALNTR
                     AMALKYAKDKGKDYKSLNLIVAHIGGGVSIYLHEKGRMVDMLSDDEGPFSPERSGRVP
                     ATKLVAACYSGQYSEREMTKKIRGKGGIVSYLNTVDAREVEKMIAEGNEEAKIIYEAM
                     AYQLAKGIGELATVVDGKVDAIIITGGIAYSEMFTSMVKKKVEFIAPVEIMAGENELE
                     SLAFGTLRVLNGEEEARIYSEN"
     gene            15969..16670
                     /locus_tag="ACGI59_001268"
     CDS             15969..16670
                     /locus_tag="ACGI59_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFG9739070.1"
                     /translation="MGKISLKSMIRLFSGFFIFAISSVLMINAHVGLMPWDVLHQGLS
                     IKLGITIGQASIMVGVVIVILDAVFGENIGWGTLLNMTFIGIFIDLVIFSGVIPHASN
                     TYIGVFMVVIGIILAAIASFLYLGVCLGSGPRDGLMIALQKKTNKSVRLVRTILEILA
                     LVVGWLLGGSVGIGTLVSALGLGYVLQIVFRIFKFDTKLLKHRFIIDDIREWKEKKSN
                     EHKCKSSIVIKNEQN"
     gene            16730..17236
                     /locus_tag="ACGI59_001269"
     CDS             16730..17236
                     /locus_tag="ACGI59_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFG9739071.1"
                     /translation="MELWDLYNADGIKTGNVIKRGNSIEEGYYHLAVEVWILNSNSQI
                     LIQKRSKSKKTLPNMWGMTTGCIVSGEDSLEGAIREAKEEIGIDITKDEMKVFRSMIH
                     EDTLWDVYLVKKEYDISKAILQEEEVSDIKWVSTDEIRQLLKEGLFFEYPEIYELLYE
                     IDNNKLNI"
     gene            17411..18433
                     /locus_tag="ACGI59_001270"
     CDS             17411..18433
                     /locus_tag="ACGI59_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFG9739072.1"
                     /translation="MEDNKGLARDSSKYKIVSISVVVIILITFWYMLNMVLLTFIMTF
                     IFYNLLVATRKRIKKFSSLNIPDSLIIIVLYALFAILLVLISYAVVPIIIVQLTELSR
                     VFSDFDVNQFAQSLGPKLYPIVSKLDFNKYISQAGLLIASTATKVGSFGVNILLAFLL
                     SLLLLLEKNEIKNFGDKLSDSKISFIYNSLVFFGKSFVKNFGEVMKVQVMIAFINSVV
                     SMIFLGFMGFPQIWALGFMIFVLGLIPVAGVIVSLIPLTVIAFNTGGITKVFGVLLMI
                     CIVHAVETYILNPKLMSNRTKLPVCFVFIILLVGEHYLGVWGLLIGVPIFMFLMDILG
                     VKFTSR"
     gene            complement(18580..18888)
                     /locus_tag="ACGI59_001271"
     CDS             complement(18580..18888)
                     /locus_tag="ACGI59_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HFG9739073.1"
                     /translation="MKNNNLNCAATNCAYNTSGYCYAGSIKVDGMQATTTENTYCASF
                     EDKYTSGITSRSNETNQVDTDNIHCEAVKCKYNKNELCKAEKVHINERNASCETFEMK
                     "
     gene            complement(18948..19376)
                     /locus_tag="ACGI59_001272"
     CDS             complement(18948..19376)
                     /locus_tag="ACGI59_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9739074.1"
                     /translation="MNNKLHELEKNLPETSCSFCTHLSFKGPSLDYRYDIRCVISDTK
                     PDFRGCCEYFEPEYTELNTGDLDNLYINFLETCLKVNYNDYINSMYWKLFKERTLLEN
                     NFKCSICGSTENVDVYHVNKNLGRETSKDVVVKCDKCNCK"
     gene            complement(19599..21056)
                     /locus_tag="ACGI59_001273"
     CDS             complement(19599..21056)
                     /locus_tag="ACGI59_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HFG9739075.1"
                     /translation="MTTFLIGLAILLIGGALYGAYCEKVFGPDDRKTPALAQSDGVDY
                     VPMKKWKNSLIELLNIAGTGPILGPIQGILFGPIAFILIPIGCVFGGALHDYMSGMIS
                     IREKGAQMPSLISRFLGNKVFQVYNIFLCLLMLLVGAVFIYTPGDLVVTQILNMKSTI
                     NNPVVWIVYGLIFLYYLCATLFPIDKIIGKVYPIFGAILLLSAAGVGIGIFTQGYDLA
                     NLSLANWKGIHPDGIPLIPTFFVTVACGIVSGFHSTQATLIARSVSNEKEGKTTFYNM
                     MILEGLIAMIWAAAAMGIYNKGIPKELVGSPDVIGLVARDLLGSIGGIIAIIGVIVLP
                     ITSGDTALRSLRLMLADYFHYDQKEKKHRVILSICIFIPVIAILIFAKLSASGFNILW
                     RYFSWSNQTIAIFAFAMITVYLIIKEKNYIISLIPGMFYSFVIFSYIFNAQIGFNLNI
                     NISYVLAAIFTVLYAILTVRSGRKLKSKADTKLAD"
     gene            21474..22388
                     /gene="nadA"
                     /locus_tag="ACGI59_001274"
     CDS             21474..22388
                     /gene="nadA"
                     /locus_tag="ACGI59_001274"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HFG9739076.1"
                     /translation="MDKDLTYQIKELKKEKNAIILAHFYQPPEIQELADAVGDSYYLS
                     EIARDCKEEVVVFCGVRFMGESAKILSPEKTVLMPVSNAGCAMADMVDEEGVIKLKQQ
                     YPNALVVCYINSTAKVKAHCDVSVTSSSAIKILENIDNKEIIFLPDKNLGGYIAEQFP
                     DKNFIFWDGYCKYHNNIRAEEIIELKDKYKNAEVLVHPECKKEIRDLGDYVGSTSGII
                     KYATNSKNKDFIIATEEGILHELKKNNPNKNFYIPGGKILCTDMKKTTLENLYSTLKN
                     MENEVIVEDEIMEKALNSLLNMHKLAEG"
     gene            22401..23702
                     /locus_tag="ACGI59_001275"
     CDS             22401..23702
                     /locus_tag="ACGI59_001275"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HFG9739077.1"
                     /translation="MNLEQDVLIVGSGVSGLYCALNLDKSLNVLVVSKSTIENNNTYL
                     AQGGISTARNIDDIKSFVEDTMKAGQYKNRVEAVQVLADESIENVGQIVEYGMPLDKE
                     NGEIDYTREGAHSVNRIVHSKDNTGEVVFKTLLKEAKTRENITLIEDAYLLDILKDGN
                     KCIGARIFKSKKEIHVFSKIVVLATGGIGGLFKNSTNQRHLTGDGIAIALRNNIKIEN
                     LEYIQIHPTAFYEENNEGRRMLISESLRGEGAKLLNKNKERFVDELLPRDVVSKAIFE
                     QMEKDKLPYVYLDATHLDSEYLINRFSFIYNECLARGTDITKECIKVSPAQHYFMGGI
                     HVDLDSKTSMENLYAVGEISCTGVHGANRLASNSLLEGLVFSKRAAKNINSVIDNVKV
                     KFIDVPDMDIDIEQVKKENKILVIKEIERTSEDFGDELFDY"
     gene            23683..24519
                     /gene="nadC"
                     /locus_tag="ACGI59_001276"
     CDS             23683..24519
                     /gene="nadC"
                     /locus_tag="ACGI59_001276"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HFG9739078.1"
                     /translation="MNYLIIDRMIQDALIEDVPSEDITTNSIVDENSKSTVDLICKQD
                     GIIAGLGVFKRVFEILGDVDVKLYKNDGDRVKNREKIAVLTGSTRNLLVGERVALNYL
                     QRMSGIATITSRYVEKLEGTNTKLLDSRKTIPNLRILDKYSVKVGGGCNHRFNLSDGI
                     LLKDNHIGAAGSVKKAVELARKNTSFVRKIEVEVETLDMVKEAIEANADIIMLDNMSL
                     EMAKQAVDIINGRAIVEFSGNVNLDTIEDIGKIGVDVVSVGALTHSVKALDISMKNLR
                     NI"
     gene            24775..25107
                     /gene="saoD"
                     /locus_tag="ACGI59_001277"
     CDS             24775..25107
                     /gene="saoD"
                     /locus_tag="ACGI59_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018942 - organometal metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE-related protein SaoD"
                     /protein_id="HFG9739079.1"
                     /translation="MKVAYIFSSTNTHKILDKMIIPQLEQGTHGVDVLGMFFFMDNTL
                     FLSKGNPVGERLSKLHEKTDMIIMACDQCAIERGIENNLVDGATIGCFPNLYACLGSA
                     GVDQVITL"
     gene            25197..25454
                     /gene="saoT"
                     /locus_tag="ACGI59_001278"
     CDS             25197..25454
                     /gene="saoT"
                     /locus_tag="ACGI59_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_240067706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018942 - organometal metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:25230..25232,aa:Sec)
                     /transl_table=11
                     /product="thioredoxin-like (seleno)protein SaoT"
                     /protein_id="HFG9739080.1"
                     /translation="MSKILVEFINTUPSCDEHGVFIKKMSEKYADKLEIKLYQAGKDF
                     SYIKKYGIITKGTLIINQKKKYDRLNKDTIERAIVEAINNN"
     gene            25481..26566
                     /gene="saoE"
                     /locus_tag="ACGI59_001279"
     CDS             25481..26566
                     /gene="saoE"
                     /locus_tag="ACGI59_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0018942 - organometal metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter SaoE"
                     /protein_id="HFG9739081.1"
                     /translation="MNILEIVLNGVKDTIVASIVTLNGASGWLMISFILAGVLHNVLT
                     PRRFQKQLGNKKFSSLIKSTISGMFLPICSCGVIPLGISMYYSGAYLGPVLAFMTSTP
                     IINPIAVVLCLGLLGPKVTIIYLIAGFLVPFLVGFLGNCFGKEELSLHNDSEEEVIEL
                     EEEKRSFMGNMIDGLKWSFGELALTISKYVVMGMVFAGFITTIFPNSIIQKYLGNPGL
                     LSLGSIAILACVMYVCAVGHIPFIAALVASGASPGIAITFLIAGAATNLPELISMFKL
                     IGKRTVIIYSVTLTLSSIIIGYITNLLLVNNNSVNLDATNSSIQLANKLMLNIPDSFE
                     YVCTILIILFAFKSIIPKMKEVIGNHA"
     gene            26559..27017
                     /gene="saoC"
                     /locus_tag="ACGI59_001280"
     CDS             26559..27017
                     /gene="saoC"
                     /locus_tag="ACGI59_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018942 - organometal metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-Cys-COOH (seleno)protein SaoC"
                     /protein_id="HFG9739082.1"
                     /translation="MLKKIVIISCIVVVLIILSKIVDDNIKEDASIPNVNKETLEYFR
                     KNYKEDIITCAEEDLNNDGKKDLVVIYKKSNNSNEMVVVVSDKNSHYITKPIPAPIEN
                     QTITFKNIDDKAPIEVIVSGSKNGNVGYAIYRVEGKKFVDLFGEDMDKCC"
     gene            27052..28143
                     /gene="saoX"
                     /locus_tag="ACGI59_001281"
     CDS             27052..28143
                     /gene="saoX"
                     /locus_tag="ACGI59_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0018942 - organometal metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding subunit SaoX"
                     /protein_id="HFG9739083.1"
                     /translation="MKLSKKIQALILFGVMGTSILTGCSSKPKEKEEAKTGKDDYTIT
                     VGYYNCDHMTAGPVAESAGIYKDLGLNVKTVGNGKVPEAMAAGKMDAGYIGTKGLVGA
                     IPKGSPITIAANNHTGGSEYLIVSKDIKEPKDLIGKKIATDMSDFLWTSDYGPETGLP
                     TDPSKYEVVNMDSDKDKYLALKTGKIQAFTSCDPWGSVAENDGAGKIIASTQYKEKAN
                     GKEYNCCSFSLNKNFIKEHPDLAKKLVLAHTKAIEYIYTNPAEAAKIFAKYYNVEEEV
                     ALRTIYKKTVGEGRTLTWKVTGEEYKNNLQMYKDLKALDDIPKYEDTIDTSLLDSCGA
                     DDFDKFIKEKVNPEFPEGMSYEDWKAKVL"
     gene            28157..28945
                     /gene="saoB"
                     /locus_tag="ACGI59_001282"
     CDS             28157..28945
                     /gene="saoB"
                     /locus_tag="ACGI59_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_239591066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0018942 - organometal metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:28370..28372,aa:Sec)
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     (seleno)protein SaoB"
                     /protein_id="HFG9739084.1"
                     /translation="MKNIIILFIIILGSIFIIFFNFEDKEEIKGKLRIGVSDDTSGFV
                     INYMVNRDYFENIKVSDIMDIFTINDCUASTMQWALSSEEIDIAIICKDAAKQYVNVN
                     SGFEIVGTVVQNSDIFLLSDNNPEKIGVIQNRDYQSELVKKYYKNVEVAPLLGTALAY
                     GLESNLVDGVVIDCIKSIGLEGKRKSTTKLGDYDTYVLVVNKEFKKSKAYSDFITLYN
                     KSVDALKNKATFKKVLKEYRNIRNIDMEEISNWKLKFLKIKAID"
     gene            28969..29712
                     /gene="saoP"
                     /locus_tag="ACGI59_001283"
     CDS             28969..29712
                     /gene="saoP"
                     /locus_tag="ACGI59_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_239591060.1"
                     /note="Most members of this family are selenoproteins with
                     the selenocysteine residue at the channel-gating position;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0018942 - organometal metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:29533..29535,aa:Sec)
                     /transl_table=11
                     /product="ABC transporter permease subunit SaoP"
                     /protein_id="HFG9739085.1"
                     /translation="MDILYKIILLVIIFLVWHFAAKDIGSSLLLPMPVDVIKGFFFCV
                     TDAETVTNLFITLQRVLKGFMYALLFGLPIGFIMGFSKTFERVLSPVVDSVRQVPIMA
                     WVPLTIVWFGIGDGPTIFLIAFSGVFPIILNTIQGVRAISKDYYNAARSMGASPIVIF
                     TNVIVPASLPDILTGSRIAISTGWMSVIUAEFIATSAGLGYSMVQAQTLMHTDVLIGL
                     MIFAALIGFIIDRVLKFINKILCRWRFAD"
     gene            29712..30464
                     /gene="saoA"
                     /locus_tag="ACGI59_001284"
     CDS             29712..30464
                     /gene="saoA"
                     /locus_tag="ACGI59_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0018942 - organometal metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein SaoA"
                     /protein_id="HFG9739086.1"
                     /translation="MKLSVRDINKTFVNNRVHTKVLEDISMDIDDGQFVCLLGPSGCG
                     KTTLLTIIGGFQKSERGDVFINDKRVKKPGIDRAFIFQNYALFPWKTIRGNVLFPMKQ
                     QKIPKEKREEMLEELLVMSDLKGKEKLFPHQLSGGMKQRVAMIRALACNPEVLLMDEP
                     LGAVDFQMRQNLQEELERIWIKKKITALMVTHDVDEAVYMSDRVIVMSRDKGRIIDDI
                     NIDIPRPRIRGSQKYEEYKNKLTDTLSKCYEV"
     gene            30477..31118
                     /gene="saoL"
                     /locus_tag="ACGI59_001285"
     CDS             30477..31118
                     /gene="saoL"
                     /locus_tag="ACGI59_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_238476746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018942 - organometal metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:30960..30962,aa:Sec)
                     /transl_table=11
                     /product="MerB-like organometallic lyase SaoL"
                     /protein_id="HFG9739087.1"
                     /translation="MSQIEKYNSLDNTQQKIRISIMDMIIDKGSSVTLQEVTEYVSKK
                     LNIEKEYIERTLQYFIYKNIMVVDGNSINFIYPVSALPTNHKVTLRDNRSFSAMCAID
                     AIGTSCTFNQDIKINSICSATGKEIEVIIKNEKIEYVNNPNLRVLHINLDKHLNWAAS
                     CUNIMNFFWTKSDFDAWTNEAGLSDDEDIYCLDINEAIVESYKIFKLKQKVLS"
     gene            complement(31254..32075)
                     /gene="yidA"
                     /locus_tag="ACGI59_001286"
     CDS             complement(31254..32075)
                     /gene="yidA"
                     /locus_tag="ACGI59_001286"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFG9739088.1"
                     /translation="MYKLIALDIDGTILNTQKRITPEVFESIQEAKRAGAKVVITTGR
                     PLPGVKELLNQLNLTDEGDYVICFNGAIIQEVKSEKIIHDVEMSLDDFDFIYNNVCKK
                     YKTKIHINTMTNLITPNETPGKYTLHEAKLNNIEVKYIQKDKIDESIKICKIMIVDEP
                     ERLEEIIQQLPKNLFNKYTIVRSAPFYLEFLGKTTNKGTALKTLCTNLNIPIENAIAV
                     GDEENDQHMIKYAGLGVAMGNARNSIKEIADYVTDTNNENGVAKVINKYILNKAI"
     gene            32568..34262
                     /locus_tag="ACGI59_001287"
     CDS             32568..34262
                     /locus_tag="ACGI59_001287"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HFG9739089.1"
                     /translation="MKFSEFKYERPNYNSMKKEFLSCVEDINNSRNYKEQQKNIHKIN
                     LLRNKIETLSNIASIRYSTDTFNKFYKEEKNYWDEYMPLYEELNSYFYNAIVNSKFKY
                     DLIKEFGEQFFTIVEYSLKSFSKEIISELQEENKLCSEYTRLLASAEIMFDGKIRNLS
                     GMGKFMYSKNRKTRELANKAYYNFFEENETKFDDIFDKLVKLRDKMSKKLGFEDFVEL
                     GYIRMMRSDYREYMIKNVRKQVLKYVVPMANELYEKQAKRIGLEYLSYIDEGVEFLTG
                     NASLKGDSRYIIKNGKRMYSELSKETNEFFDFMLENELMDLETKKGKGAGGYCTYIPD
                     YKSPFIFSNFNQTADDIDVLTHEAGHAFQLYMSRWIDMPEINFPTLDSCEIHSMSMEF
                     ITWPWMDLFFKEDTDKYKFTHLSSSIKFIPYGVIVDEFQHYIYKNPNVDKSKRKEIWR
                     FLEKKYLPHRKYGDNSFLERGCWWFKQGHIFKNPFYYIDYVLAQICALQFWKKMIQDR
                     DAGWKDYINICKVGGTKSFLDIVSMGNLYSPFDDGCIESIIGDVKSWFDEINDSKL"
     regulatory      34594..34704
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            34808..35179
                     /locus_tag="ACGI59_001288"
     CDS             34808..35179
                     /locus_tag="ACGI59_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrdX family protein"
                     /protein_id="HFG9739090.1"
                     /translation="MIIITNNPKVKEEVQGREVLFKDTTYIGILEASRDLIHEGYELL
                     SHPLYGSVKPNETPYRTVILKKGNRLDINSLTLIEEAIITASKFQNNKKTPKWTESVQ
                     DDFRVIDYDIFYNTIQRMQYE"
     gene            35274..36221
                     /gene="trxB"
                     /locus_tag="ACGI59_001289"
     CDS             35274..36221
                     /gene="trxB"
                     /locus_tag="ACGI59_001289"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFG9739091.1"
                     /translation="MENVYDLVIIGSGPAGLAAGLYGARAKLKTLILEKDKTGGQIVI
                     THEIANYPGSVPNATGPSLIARMVEQCKEFGAEMLRDNIVDTELDGDIKVLKGEKAEY
                     RAKAVIIGTGATPRKIGCPGEKELTGKGVSYCATCDADFFEDFEVFVVGGGDSALEEA
                     MYLTKFARKVTIVHRRQGFRCAKSVEEKAKANPKIEFLLDTVIEEIKGDGILESVVFK
                     NKVTGETHEYFADEEDGTMGVFVFVGLDAQTDLFKGKVDMDEKGYIITDEDMRTNIPG
                     VFAAGDCRSKTLRQVVTATNDGAIASIVAEKYIDEKFGN"
     gene            36325..36642
                     /locus_tag="ACGI59_001290"
     CDS             36325..36642
                     /locus_tag="ACGI59_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HFG9739092.1"
                     /translation="MLDLDKATFEEEVLNAEGFVFVDFWSEGCEPCKALMPDVHKLAE
                     TYGDKIKFCKMDTTKARRLAIKQKVLGLPTMAIYKDGEKVDEVTKDDATVPNIENMIK
                     KYL"
BASE COUNT        14501 a         3858 c         6340 g        11955 t
ORIGIN      
        1 gatattactc tagtttattt taaaaaaaag tattataata ttccttgtgt gaaaatatag
       61 gaatttttat taaatagttt taatgtgtaa ataacattct acactattgt aattaaatat
      121 gtaaaaagta aaatatttaa atagatatat tttaaaatct aacttttaat attgtaaaaa
      181 aattagaaat ttaacaaaaa gtacaaaaat tcacattttt ttcataaagt gtttgactaa
      241 aaaattcaca tgttgtagaa ttaaggtagc aggagttttg agggaggtgt aaataatgat
      301 aagaaaaata ccacaagcag aactaaaagt aatgaaattc atttggaaag tagatgttac
      361 agtgacatca aaagatgtta ttgaagctat ggaacaaaaa tatggctgga aacaaaccac
      421 cacactgaca cttttatcta ggctggtaaa aagaggattc ttagatgctc aaaaaataga
      481 cagatataca cattacacaa taagagtaaa acacaaagat tatttaagtt ttgagacaaa
      541 ggatttccta agtaatatac atgatgactc tttaagaagc ttaatttcag cattacatga
      601 tgatgaaaat ataggtaaag ataagctaga ttttctagaa agttattttg ataatttaaa
      661 ggatgaataa ttatatgcta tatgtttttg aaaatcttat aaagacaact attgtatgta
      721 gtcttggtat ttgtctatta ttatttttga aaagatattt atttaagaag tttagtaaga
      781 gattcaatta ttatatttgg ttgataatag tctttagaat gttacttttt ctatttaatt
      841 atacaatagt gtatgaagta aaagaaccca aagaaaatgc attaggaaat aatattacac
      901 aaattgatat ttctacagat aataatctta tgctgtatgt agcttattta tggttatttg
      961 taactatagt cattgcagtt tatacattta taaagtatac aagatttaaa aatctagttg
     1021 ttgatgtatc ttatgatatt gaagataatg atataaactg tatctacaaa aacttgttaa
     1081 aagaattaaa tataaaaaag aaaattgaat taagaggttc tgatgaattg ataagtccag
     1141 caggtatggg attatttaaa tcttacatat tcttaccaga ctatccttat agtaaagatg
     1201 aactaacttg gattttgaaa catgaattaa tgcattttaa aaataaagat attttaataa
     1261 agtttttagt tttgtctgtt agaataatat attggtttaa tccacttgta tatgtaatga
     1321 gcaataaagt aaatctagat tgtgaattat gttgtgatga aagtgttttg gtagaatgtt
     1381 ctttaaaaga taaaaaagaa tatgcactag cacttataaa gtcaattaag tttagtaaaa
     1441 attataatag taagattttg acaaccgagt ttgataagac aaatttggaa aagagacttg
     1501 agagtattgt taaaaagaaa ggcaaaagtg gaatactaat tgctgtttta gtatttatga
     1561 cttctataac atcttttgtt gaagttgatg ctcaagttag aattgatgag ggtaataatg
     1621 gaatgaataa caatattaat gaatttttat tgaataagac gactgataaa attataaata
     1681 tttattataa agatgcacca aatgaaatta ggaaatttta tgaagataaa tgcgaattag
     1741 aaggtaaaat acctagagac tatgacataa tagaaatgga tatgaaagat tataatcaat
     1801 taataaaata attataagga ggattttatg ttaacaaaat taagaaaaat agtagctatt
     1861 atgtgttgct tattgcttac ttttacaagt gtggcatttg ctgacacaag taaagagaaa
     1921 ggaggaagta aagagactgt tccagagaaa aacctagatt cttttactct ttctgggtat
     1981 atatatacat atgaaaatgc gccacaagaa ataagagatg aacataaaca aaattgtgaa
     2041 gagataaata tagaccctaa gccagatgat gagatatttg ttccagaatc agctaatcta
     2101 atgaatgagg acaatagtaa aggtgtttat gcaagttata ctgtttctta taatataggt
     2161 gctaaaacta taacaattat gagtaatgag tatttgacta taagtacaac aaaagttata
     2221 cgaaagggta atagcggaaa agaagtaaaa gctgcacaaa tcatgttaac tttacttggt
     2281 tataatgtag gtatagatag ttcttttgga agtaagacat ataatgcagt tgtaagtttc
     2341 caaaaaaaat acggtttgtc tgcagatgga ataattggtc cagctacttg ggataaattg
     2401 ggtagactaa ctgaccctac tctttcatag gattaaaatc taaaaatata ttaaatagtg
     2461 tctcaatatg atttttcaat ttaaatgaga cactatttaa atataaatgt tataaaacta
     2521 ttttacaata aattccatat aactctacca tctatatcct taacattttg acaaccagtc
     2581 aaaatcatag tctcacataa ctcattccta accttctcta taaaaaactc cacaccatca
     2641 agaccaccgc caaaagaagc agtaacaaaa ggtcttccca tcaaaacagc atcagcaccc
     2701 aatccaagca tcttaactac atcaacacca gtcctaactc caccatcaac aagtattgtt
     2761 atctttccct ttacagactt agctatatct ggaagtacct cacaagtacc aggagtataa
     2821 tcaagtactc ttccaccatg atttgaaaca ataatggcac tagcaccaga ctctacagcc
     2881 atcaaagcat catctacagt cataattcct tttataataa aaggtaatct agtggattca
     2941 accaattctc taatctcatc aattgtttta ggagaaaaag gattttcttg aaattgatta
     3001 ttaataagtc cacaagcatc aaggtctaca ccaactgcaa aagcacctgc ttcttcagac
     3061 aatctaattt tatctattat ctttgaatta ttccaaggct ttataaatac aattccattt
     3121 cccctattat ccttcaatac atctaaatta tccagtaaaa aagtatctac attagtatcg
     3181 ccaaccatag cataaattcc agaattagaa caacctctaa caacaggctc tatgtattct
     3241 ttttcggaaa ctttaccacc catgttcaat atagtacctg atacaggtgc agcgaatata
     3301 ggagaactca tttttctacc aaataattct atggatgtat ctggctttga aacattatgt
     3361 atgactctca tatttatctt tattttttct agactttttc tattttcaat gaaagacgaa
     3421 ccacttcctt taccacccat accaggaaca tctccagcac aagctaatcc attacaaatt
     3481 ttacatactt tacatgttcc attaaagttc tctctagctg attttagtaa gtcattgtaa
     3541 tccatacaaa atctctcctt tttataatat taaaattgag tattactata ttgaaaatat
     3601 cttaattgta aatataggga tgttattatg atgagctaaa ctagataata catattagat
     3661 attttttatt gttgtagata gttaaattat atcatattag atagtcaaat tttgaaaaaa
     3721 actaacttat ttaagtatac attttgaaaa aattaaaaaa gctatatata gatttttaaa
     3781 gaaatctata tatagctttt aagtttatgt caatcttttt tgttaatgaa taaataatat
     3841 agaatacaat atacaaaaaa gtttcttgaa atgctaagat aatccccatt tacaaagcaa
     3901 catcattata ctgtgtaaat ttgaatcaac aatgttttca ccatatttca tcataaaatc
     3961 ctctaattgt gtgtcaataa atgaataata tccacttata ctatcgatgt atgcagtcat
     4021 taagtgtata cattcatcac ttttatcttt atacatatat atctccccct atatacaata
     4081 tataaattta gataccaaat cgtatcatat taaatagtta agtgtgtaac atctatgcag
     4141 ttatatattt atatcattat attaataact aaagtgagtt ttgacaatag attttgggaa
     4201 actggatgtt caaagtgcaa acttggattt taaaatcatt aattaagata gcgcatagga
     4261 atatatgtat acataactta attataaaga ttataaacaa ttacttttat taactttatt
     4321 taaatggtag aataacttgg agattaataa ttataaggag gatataatat gagtttatat
     4381 aagtttatat atgatgataa ggaatattta ttaaaagagg agaactgtag tgctttaata
     4441 aatgatgaag aaaatccagt tcaaggagta agtatttcaa aaataattga tatacttaat
     4501 gaagcagaag aagttgattt tgatgtagaa tattatcaag aagcatgtcc attatgttta
     4561 gaaggagtaa aagaaaagaa aaaattcttt ccttttttag aatatcactt ttatattttc
     4621 agtaaagatg gagaatatgt aataagcaat atatctgttg attataaagg actatctttt
     4681 aataaactat caagggcaaa taaagttgat aatagttata ttgtaagtgt gataatttgc
     4741 gaaaattgtc aggattatat agttcagata gaaaattgta tagtttagtg taaagtatgt
     4801 cttaatttaa gagaaatttc taaattgaga catcaacttt tttcaaaaat agtatggaaa
     4861 attactaaat ttatgagata atgtaagtcg gaggtgagtt acaatatata gtaaatattt
     4921 ggaagagata gatgatacaa ttttagattc aatgtttaaa ctaaccttga atgaaagatt
     4981 attgactaat aacgatttta ttgaaaataa aattgatgta gtaaagaatt taattgattg
     5041 ctataaaact gaacaaaata gtttaatgtt attaaaatca aattttttgc tagctttatt
     5101 atatggtatt caagggttta gtgaaaaaat gaaagagtat attttgattt cctgtaaata
     5161 tattgataaa tgcttaccca aagattataa atttttagca cgattttatt cacaaatagc
     5221 tattttaagt ttaaaaaatg aagatgtaaa taaggcaaat ttatatatgg ataaatttaa
     5281 tcttatttgt gatgaaaata attttctaat tgaagaaatt atttttaaat cacaacttat
     5341 ttacattaag gcaagtaagt gtatagaatc ttctgtaata attaaagaaa tagaaacgtt
     5401 gtatataaag ataaaagaaa ttaacgattt taattgtaaa aaaatatatt tttttattat
     5461 tggtaaaata tattttttgt ttttggatga tgctctaatt gctaaggtaa attttttaaa
     5521 agcaagaaaa tatgcagatt tactaaagga tatgcaagta tcttcactat gtaatataaa
     5581 gataggtgag tgtgaatgtt cgtttgaaaa ttatgaatgt tccatggaat atttcaatga
     5641 agtaataagc aataaaaagt atagaaatgt aaatataatt caaaaatata gagcatcaaa
     5701 caacataact aaaatattaa taaagactag aaattatcct gtcgcaataa ataatttagc
     5761 aaaatctgaa gtatatctag aaaaaattaa aaatcatagc ttaaaagaag tagagaagtt
     5821 tgatttattt ataagtttag ccatgtatta tgcaaaaagt aatgaaaaat cttttgaaca
     5881 atcaatatgc tatttagata gagctaaaaa catattaaat atgataaaaa atatagaaga
     5941 atatataata tatgatttag aaataattta tcaccaggca aagatatatt atatctttgg
     6001 atattatgac aatgccttaa atacaaataa aaaactcctt gaaaaatcta aaaatgctaa
     6061 aaattataat tatgtgaagt tagcatacaa gagtatatat ttgtgttttg aaaagattca
     6121 aaattatgaa atgagtatga aatactttaa aatgtattat gagttaaaaa tgatgtatat
     6181 aaaagctcga aatagaaatt atatagattc cttaaattat agacatgagt atatagaaaa
     6241 agaaataaat aacatgagaa aagtaaaact tgatttagat aaaaaaaagt atgttgacca
     6301 tttgacaagt gcatataata gaactttttt gaacgatttt ttagaaaaac aagaagttag
     6361 tgaatattgc acagcattta tgatagatgt agattacttt aaagaataca atgatactta
     6421 tggacattat aatggagata ttgctcttaa aaatataact ataataataa aaaaatatct
     6481 aaaaaaagac atgcttatga taaggtatgg tggagaagaa tttctgatat tatctgtatg
     6541 taaagattat aaaaaaagta aaatatttgg aagaaaatta tgtaggattg taaagaaatt
     6601 tttaaataat aatttgacag ttagtattgg cattgataca tgtaaaaata gtagtataga
     6661 tataaaagaa ataattgaaa atgctgataa agctttatat aaagcaaagg aaagtggtag
     6721 aaatagatgt ttgcattatc atgattttaa gaatttttaa ctgttgaaat aggattggtg
     6781 atttacttga aaaggagttt aatagaagat ttaaagagaa atataaacaa gatagaatac
     6841 gatatgttga ctaaattcta tctaaatttg acagatttaa actacatctt ggattatcac
     6901 aaagaccttt taaatacaaa attagaagat aatagtagag tctatacttt atatttaatt
     6961 agtcttataa actatataaa aaaagataga gaagcatcat acaactattt gatagagtca
     7021 ttaaagtata ttaaaaaatt gtcattaaaa gatgattcag atataatatc taaaatttat
     7081 atttatctta ctttatcagc tatttctata agaaaatatg ataaggtaaa taatttttat
     7141 ttctcagcaa aaaaaattat aaggcaaaat catttaaatg agttgttggt attgttaaat
     7201 atgagtttat gtgtggaaat aagtgcttta tatcagacca aaacggatgt aatagcttta
     7261 gtagaagaag tgtctttttt taaaacttta aaaaataaag ctcttgtaag tagggcaaat
     7321 tatatatttg ggagagttta tctatattta tttaataact ttataaaaag tatggaatac
     7381 ttaattgaat gtctaaaatt ggctcaagaa aataatttat actctataga ggctttttgt
     7441 aaatgtataa taagtttgtg ttatttagag agtggaaata atgtagaagc tataaaatac
     7501 ataaataatg tattagatgt tgcacacagt aataactcaa tatcagtaac tgaaagagtg
     7561 tctataaaaa tagaccttat atgggcatat ttaaaagaag atatgaataa agaagcagaa
     7621 gttattttat taaagactat aaaattaatg aaagttgttg aaggtgtata taaggattat
     7681 ttatattcat ttatattttt atattcagct gaaattgagt taaagaaaga taattgtagt
     7741 tttgaaaagg taaacaccta tttaatttta tcaaaaaata tatataaaag ttttggagat
     7801 gtttttatat ataattctaa actagattac atatataagt tgtatggaga tttatatata
     7861 aaatttggta atatagatga aggtataagt tgttatataa aaggtttaaa tttagttaaa
     7921 aagagttcat tgaatcttag aaaaatatct atatttatat gggctaatag caaatggata
     7981 tgagctaaaa aaagatttta aactggcaat agaatattat aaaagtatgg attggtattc
     8041 tgataggtgg gaaaaaaata attcctataa aacatctcaa gcaattcaca aacaatatga
     8101 actcagacaa aaacaagaga gtttaagatt actagtggat gataataaga aattagaaaa
     8161 tgatatttta aaagatggac ttactaaact atataataga agatatttgg aacagcagtt
     8221 ggatttattt aatggaacca gagacaaaaa tatggtcgca atactggtcg atatagattg
     8281 ttttaaaagt tataatgata attatggtca tctagcaggg gataaagtta tagttaaggt
     8341 aactgatatt ataagaagtg tatttataga tattaccgaa cacataatca gatatggggg
     8401 agaagaaatt ttaatattag tagattctat agaatcaatc ggtgttgaag agattgataa
     8461 tatagaatta tatattaaaa gagttttcaa acttcttgaa tttgagggta ttgaacatat
     8521 atattctaaa ataaaaaact atataactat aagtgctgga gtaagtataa aaaggtgtcg
     8581 tagtagaaaa gatgttgaaa tattaattga agatgcagat aagaacctat ataaatcaaa
     8641 aaaagcaggt agaaatcaat acattataat ttaaaattga ggtgattttg ttgcgaaaaa
     8701 aaataggaat tatagcctct gatattgaat taaaagaaag aatagaagaa ttatatagag
     8761 aagatgtaga aaatggaact ataataatcg acatacttaa cctagactta atggaaaatc
     8821 aaggtcgtat acttgttgaa aaaggtgccc aagctataat tggtagaggt ggaggttata
     8881 gcttagtaat agatacagta aatgtacctg ttataccaat gaatatgaag tcaactgacc
     8941 ttttaagagc aatagaaata gcaaagaaat attctaaaaa agtagtattg attcttggag
     9001 acaatgaagt ttcttttgac tatgttggct ggagaaatgt cataagtact gaaataactg
     9061 aagaatggtt tgaatctaag tatgagataa gaagtaaagt tgtaaaatat atagaccaaa
     9121 aagatgaagt tgtaatcgta ggaggaggtt tggcatgttc ttttgcaagg caatatggaa
     9181 ttgatagtgt atttgcaact gctagtgatg aatctataag agaagctgta gaatattgta
     9241 aaaaattatt agatacatta ggtgaagaaa agtttaataa tgaagtgtta cgaaatatac
     9301 ttgatggtat aaaagatgga gtaattgcta tagatagtaa tggcagtata attttgtaca
     9361 atgaaagtgc aaaaaacatg cttaaagtag agagaaaatg tgcacttaat aaatacatct
     9421 tagatgtatt tcctaaaatg gagtggatgt tagattgttt acatgaaaaa gaggacgtag
     9481 aagatagaaa gataagaaat ataaataatc taattgttaa tactaggaca acattgataa
     9541 aagttgataa tagtacttat ggagttttag gaataataca agatataacc aagctacaaa
     9601 acttagaaag aaaaataaga tttgacttga atcaaaaagg tctctatgct agatatactt
     9661 ttgatgattt tttatttaaa gataaattaa ctaaagaatt tattgaagaa gctaaaaaaa
     9721 ttggaaaaag tgattataca actttacttt atggagaaag tgggtctggt aaagaaatta
     9781 tagctcatag tatacacaac ataagtaaaa gaaaagatag gccttttgtt gctataaatt
     9841 gtgctactat agcagaaaat ttacttgaaa gtgaactttt tggctatgaa gaaggtgcat
     9901 ttactggtgc tagaaagggt ggaaaaagag gactatttga acttgctcat ggagggactc
     9961 tttttttaga tgaaataaat agtctatcct ttaatattca aacaaaacta ttaagagtaa
    10021 tagaagaacg tcaaataatg agaattggtt ctgattatat aatacctctg gatataagga
    10081 ttatagcagc aacgaatgaa tctttaaccg aaaaaatagt tatgggtaca tttagagcag
    10141 acttatttta tagattgagt agtttagaga taaatatacc tcctttaaga gacagaagag
    10201 aagatataat acctttgttt aacaattttg taaatgaagt attaaaggat gatggtctca
    10261 atggaataaa cagtatagat gaaaattttg ttttgactaa agatgaaatg gataagctat
    10321 ataattatag ctggcctgga aatgtaagag agttaaagac aatagctcaa aaatatgttg
    10381 tgacaggtaa aattaaattg agacaagata gagattttaa aactaaaaaa ttacttccta
    10441 actctgaagt agacaaattt aattctgaaa cgacagcaag tgtagaagtc caagatgaat
    10501 ctataaatat aagtaagata aatgatggta agataagtat agatataaaa gaagtaaata
    10561 agtatgttga agaaaaaata ataagtatgc tttttgctca gggactttct aaaaatgaag
    10621 ttgcacaagt tcttggaata agcaggacat ctttatggaa gaaatacaat aaaaatattt
    10681 aaatataatt acatataaat tattttgaag taatgttaat tttttaaaac aagtgttaag
    10741 aaaaattaac attacttttt tgcaaatttt tattttttta gacaggcata tatatttata
    10801 agaaaaatag ttaaaacact taatttaata atgaaaaaaa taataattct aaacaaaatc
    10861 aattttacat aaagtgggat ttattggcat agtaattgct taatatataa tgtaagagta
    10921 aatacaattt taggggtgat ggtatgagtc aaagtatggt tggtaagcat gcaatgtggc
    10981 caaaagaaaa tgatgttata tttagcatat ctggcagagc tcaagcagca gagaaggctt
    11041 ttggtatgga taatgtaata aatgcaacaa taggcgctct aatggatgat tcaggaaaat
    11101 tgataacaat gaaaactgta tatgaagaat ataaagcatt agataactgt gagataggtg
    11161 catatgcagc tcttgaaggt caaccagatt acttagaagc agttaaaaaa gtatttttca
    11221 gagattactt accagaagga catataagag tattagcttc accaggagga agtggtgcaa
    11281 taaaattagc tgtatggaac tatactaatg aaggagatga ggtattaaca tctgattggt
    11341 tctggagtcc atatgtaagt atagctgaag aggctaatag aaaagtagtt aattaccaac
    11401 tttttgatga aaatagaagg tttaattttg agtcgtttaa agaaaaattt gtaaatatag
    11461 cagaaaaaca aggtagagtc tttacaatta taaacacacc agcgcataat ccaactggat
    11521 atagtgttgc agatgatgag tgggataaaa tattagactt atcaaaagaa gtcgcaaaag
    11581 ataaagacaa aaagataata tttttcgtag attcagcata tattgacttt gcaggtgatg
    11641 atgatgtatg tagaaagttt tttaagaaat tttctaattt gcctgaaaac gttctcgtat
    11701 tggttggatt cagtatgtca aaaggattta cagcttatgg tatgagaatg ggagctataa
    11761 tttgtatatc ttcaagtgaa gatgtagctg aagagttcca ttactcatgt gttcattcat
    11821 gtagagcaaa ttggtcaaat tgtaatagaa gtgctatggc agtattatct aacattgtaa
    11881 atgaccctaa aaaattcaaa gaatatgagg atgaaaaaga aatatacaaa aacatgctta
    11941 ctagaagagc agatgtattt gtaaaagaag ctgaaagagt aggattagaa attcttccat
    12001 atatagcagg tttctttgta agtataccat gtgataatcc aaaagaagta tgtgaagaat
    12061 tgactaaaca taatttattt gcagttccat taaaaatggg cttaagattt gcagtttgtg
    12121 cagtttctga agataaatgt aagaaagcac caagtattat aaaagaagca cttgaaagtt
    12181 tagaagtaaa aataaataat taatttcatt gaacaaattg ggtggactat gaatttaata
    12241 attgtagatg gagggaattt tatatgaaac aattgatgac aggtaatgaa gcaattgcac
    12301 gtggagccta tgaggctggt gttaaatatg catctgcata tccagggact ccaagtacag
    12361 aaatattaga aaatatagca acatataaag atgcaatagt tgcagagtgg gctccaaacg
    12421 aaaaggtagc acttgaggct gctatagggg gttcaattgc aggagcaaga acaatggctt
    12481 ctatgaaaca tgttggtttg aatgttgcag ctgatccaat atttacttat gcatatacag
    12541 gggtaaatgg tggtatggta cttattacag cagatgagcc gggaatgcat tcatctcaaa
    12601 atgagcaaga caataggatg tatgcaaaat ttgcaaagat acctttattt gaaccatcaa
    12661 ctagtcaaga agctaaagat atgataaaag aagcttttga agttagtgaa aaatacgata
    12721 caccagtttt atatagagtt acaacaagac tttgtcattc aaaaggatta gtggagtgct
    12781 atgatagaga agaagttgaa attaaagagt atgttaaaaa tgctaagaaa atggtaactg
    12841 ttccagcaaa tgctcaaata agaaggggcg ttgttgaaga aagaatggaa attcttaaaa
    12901 aattctcaaa tgaaactgat ttaaactact atgaaataaa tgatacaaaa ataggtgtta
    12961 tagcatctgg tatgtgttgt aattttgcca aagaagtttt tggaaaaaat gcatcatata
    13021 tgaaactagg atttacaaat ccattacctt atgaaaaaat aaaagaattt gcagaaaaag
    13081 ttgataaaat atatgttata gaagaaaatg acccatttat agaagaacaa ataaaagcat
    13141 atggaataga ttgtattgga aaagatgtaa taccaccata tggagaaatg acaccagatg
    13201 ttttaagaaa agctatattt ggaaaaacaa atgatactat agaatacaaa tctgaattag
    13261 taacaccaag accaccaagt ttctgtgcag gatgtccaca tagaggattc ttctatgaac
    13321 ttggaaagag aaaaaattta atagttggtg gagatatagg ttgttatact cttggatttg
    13381 ctcctccata taatggaata gattatgttg tttgtatggg ttctgctttt ggaactgcac
    13441 atggagctca aaaagttctt aatatgaagg atgataatga aaaaagattg gtaggtgtac
    13501 ttggagactc aacattcttc catacaggaa taaatggact tttagatgtt gtttacaata
    13561 gaggtaattc tatttcggtt atattagata atagaataac tggaatgaca ggtcaccaag
    13621 aaaacccagg ttcaggatat actttacaag gtgctaaaac taaagaagtt gatatagaag
    13681 gattagttaa agcttgtgga atagaacatg taagagttat aaaccctaat aatttaaaag
    13741 aagtaaatga agcactagat tgggctttag ctatagaaga tgaaccatca gtaataataa
    13801 caagatggcc atgtgtactt aaaaaattct ctaaagaaga tatagaagaa tttaataatc
    13861 catttaagac taagtgtaaa gttgaccatg ataaatgtat aggatgtaaa ttatgtttga
    13921 aaactggatg tccagctcta tcatttgata aagagaataa gttatcaaat atagatagaa
    13981 atcagtgtgt aggatgtgga gtatgtgcac aagtttgtcc aaaacaagca atagttaagg
    14041 aggaaaaata atatgactaa gagtatactt ttggtaggtg taggaggtca aggaactata
    14101 cttgctagta aattattaac tatgggattg atggaagctg gatatgatgt aaagatgagt
    14161 gaaatacatg gaatgagtca aagaggaggt tcagtttcct cacaagttag atatggagat
    14221 tgtgttcatt ctccagttat agaaattggt ggagcagata tattagtatc ttttgaaaaa
    14281 atggaagctt taagatggtt caattattta aaaccagaag gaaaagcttt agtgaataat
    14341 cataggatag attctatgac tgttttaatc ggaggagctg aatatcaaga aagtgatata
    14401 gatgcagagt taagtagatt aaatgcaaaa gttataaatg cagctgataa agctgaagaa
    14461 ctaggaaatt caaaaataat gaatgttatt ttattaggat gtctagttaa atctatgcaa
    14521 ttagagtcta ttgattggga aaaaattata agtgaaaatg ttaaaccaaa atttgttgaa
    14581 ttaaacataa aagctttcca tgaaggtatg gaaatggtta gcaaatagtt tataatagat
    14641 ataaatagac tatagaaatt aaggtgaaag gaagaaaatt atgacttaca gaatattagc
    14701 cataaatcca ggttctactt ctacaaaaat agcagtatat gatggagaag aacaaattct
    14761 tgtgaagacg atagaccatc cagctgaaga gattgcaaaa tataatacta tacaagacca
    14821 gtttgaaatg cgtaaggaag cagttttgaa tattcttaaa gaaaatagta tagacttaaa
    14881 atctcttagt gcaatagtag gaagaggtgg agttttacca ccagtaaaat caggagcata
    14941 tttagtaaat gaagaaatga ttgatgtact aagacataga ccagtacttg aacacgcttc
    15001 caatttaggt gctgttgtgg cacatgcaat atcagaacct cttggaatca actcatatat
    15061 ttatgattct gttgcagtag atgagcttat agatgtagcg agaatatctg gactttgtgg
    15121 aatggataga tcaagtgcag ggcatgcatt aaatactaga gcaatggctt taaaatatgc
    15181 taaggataaa ggaaaagatt ataagagctt aaacttaata gtagctcaca ttggtggagg
    15241 agtaagtatt tatcttcatg aaaaaggaag aatggttgat atgctatctg atgatgaagg
    15301 accattttct ccagaaaggt caggaagagt acctgctaca aaattagtgg ctgcctgtta
    15361 ttcaggtcaa tattcagaaa gagaaatgac taaaaagata agaggtaaag gtggtatagt
    15421 ttcataccta aatactgtag atgctagaga agttgaaaaa atgatagcag aaggaaatga
    15481 agaagcaaaa attatttatg aagcaatggc ttatcagtta gcaaaaggta ttggagagtt
    15541 agcaactgta gtagatggaa aggtagatgc tataattata acaggtggaa ttgcatattc
    15601 tgaaatgttt acttcaatgg ttaaaaagaa agttgagttt atagcaccag tagaaattat
    15661 ggcaggagaa aatgagttgg aatcacttgc ttttggaact ttaagagtac taaatggaga
    15721 agaagaagct agaatttata gtgaaaatta ataagaaaaa ctaaaaatgt ttatatacta
    15781 aaaaagtggt atatccgttt tttatacacg aaagcttaga gtataggtga aaatgtataa
    15841 aatttggatt gccacttttt ataattaaaa ttttatctta acctaataaa taattctgta
    15901 aaatttcgta tttatatata tattttattg gtatttattg ttattattat tatgaggagt
    15961 gatttttgat gggaaaaata agtttaaaaa gtatgataag attattttca ggttttttta
    16021 tatttgcaat ttcttctgtg cttatgatta atgcacatgt aggtcttatg ccttgggatg
    16081 tacttcacca aggtttatct ataaagctag gcataactat aggtcaggct tctataatgg
    16141 taggagttgt aattgttata ttggatgcag tttttggtga aaatatagga tggggtactt
    16201 tactcaacat gacatttata ggaattttta ttgatttagt aatattttct ggagtgatac
    16261 ctcatgcaag caacacttat ataggtgtgt ttatggttgt tataggaatt attttggcag
    16321 ctatagcaag ttttctatac ttaggtgttt gtttaggaag tggaccgaga gatgggctta
    16381 tgattgctct tcagaaaaaa actaataaat ctgtacgtct tgttagaaca attcttgaaa
    16441 tactagcatt agttgttggg tggttattag gaggttctgt aggtattggt acgcttgtaa
    16501 gtgctttagg tttaggatat gtattacaaa tagtgtttag aatatttaaa tttgacacaa
    16561 aattgttgaa acatagattt attatagatg atataagaga atggaaggaa aagaaaagca
    16621 atgaacataa atgtaaatca agtattgtaa taaaaaatga gcagaattaa ttatgctaaa
    16681 atatatttgt agtttatcta atttatagta aagtcaagga gattttgaaa tggaattatg
    16741 ggatttatat aatgctgatg gaattaaaac aggaaatgtt ataaaaagag gtaattctat
    16801 tgaagaaggt tattaccatt tggcagttga agtgtggatt ttaaatagta actctcaaat
    16861 tttaattcag aaaaggtcta aaagtaaaaa gacattgcca aatatgtggg gaatgacaac
    16921 tggatgtatt gtttctgggg aagacagttt agaaggagca attcgcgaag caaaggaaga
    16981 aatagggata gatataacta aagatgagat gaaagttttt agaagtatga ttcatgaaga
    17041 tactctttgg gatgtatact tagttaaaaa agaatatgat atatccaaag ctattttaca
    17101 ggaggaagaa gtaagtgata taaagtgggt aagcacagat gaaataagac aattactaaa
    17161 agagggtttg ttttttgaat atccagagat atacgaattg ctatatgaga tagataataa
    17221 taaattaaat atatgatgta gagcagattt cagtataagt aagattattt aaaatgtatc
    17281 ttctctaaat tttaatattg ttttatgaaa attaataagt tatttttagt atttttctac
    17341 ttgttttttg agattaaaat aaaaagggca gtcattataa agtcataaaa atttggggag
    17401 gatttaaact atggaagata acaaaggatt ggcacgtgac agttcaaaat ataaaattgt
    17461 ttctatatct gtcgtagtaa ttattctaat tacattttgg tatatgttaa atatggtgct
    17521 tttaacattt attatgactt ttattttcta taacttgctt gtagccacaa gaaaacgaat
    17581 taagaagttt tcttctttaa atattccaga ttcattaata attatagtgt tatatgcact
    17641 atttgctata ttgttagtat tgattagcta tgcagttgta ccaataatta tagttcaact
    17701 tacagagttg agtagggttt ttagtgattt tgatgtaaat caatttgctc aatctttagg
    17761 accaaaattg tatcctattg tctcaaagct ggattttaat aaatatattt cacaagcagg
    17821 gctacttata gcatcaacag caacaaaagt tggaagtttt ggagtaaata tattattggc
    17881 atttttactg agtttactcc ttttacttga aaaaaatgaa attaaaaatt ttggagataa
    17941 actttctgat agtaagattt cttttattta taattcgctt gtattttttg gaaagagctt
    18001 tgttaaaaat tttggtgaag taatgaaagt acaagttatg attgccttta taaattctgt
    18061 tgtttctatg attttcttag gtttcatggg attccctcaa atatgggctt taggatttat
    18121 gatttttgtg ttgggtctta ttccagtagc aggagttata gtgtctttga ttccattaac
    18181 tgtaatagca tttaatacag gaggaataac aaaggttttt ggagttttac ttatgatttg
    18241 tatagtgcat gcagtagaaa catacatatt aaaccctaag ttgatgtcga atagaactaa
    18301 acttccagtt tgttttgttt ttattatttt acttgtagga gaacattatt taggtgtatg
    18361 gggattacta attggagttc caatatttat gttccttatg gatattctgg gtgttaaatt
    18421 tacttctagg taatatatta aaataataat ttatctttat atataaatta taaattgttt
    18481 taaaaaagaa cctataaata gacatcatta ataaagacat ctatttgata ggttcaattt
    18541 tgctttgtat aaatactata aaactaaata aaaagaaatt tatttcattt caaaagtttc
    18601 acaacttgca tttctttcat ttatatgaac tttttcagct ttgcaaagtt catttttatt
    18661 gtatttacat tttacagctt cacaatgaat gttatctgta tcaacttggt tagtttcatt
    18721 agaacgacta gttataccag atgtatattt gtcttcaaaa gaagcacaat aagtattttc
    18781 tgtagtagtt gcttgcattc catctacttt tatactacca gcataacaat atccactagt
    18841 attataagca caattagtag cagcacaatt taaattatta tttttcataa ataacctcct
    18901 aagttttatt taaacttatt atgtgaagtt atctaaattt tatacactta tttacaatta
    18961 catttatcac atttaacaac tacatctttg gaagtttccc ttcctaaatt tttattaaca
    19021 tgatacacat caacattttc agttgatcca catatagaac atttaaaatt gttttctagt
    19081 agggttcttt ctttaaataa cttccaatac atagaattga tgtaatcatt ataatttacc
    19141 ttgaggcatg tctcaagaaa gtttatgtat agattgtcta aatctcctgt atttaattct
    19201 gtatattctg gttcaaaata ttcacaacat cctctaaaat cgggctttgt atctgaaata
    19261 acacatctaa tatcatatct atagtctagg ctaggcccct taaatgaaag atgtgtgcaa
    19321 aaagaacaag atgtttctgg tagatttttt tcaagttcat gtaatttatt attcataagt
    19381 attcctccaa aaaatttatt ttaagtatat aaacgaatag attattttaa aacttttatt
    19441 tatttatatt agagatttag gataaatatt ccaataagga gtaaatactt aagtatttat
    19501 tactgtatta aataaaaaag aggcaaatct aacttgagat tgcctacttt tttatcattt
    19561 tttaataaaa tttattctaa gtattatttt atttagattt agtcagccaa cttagtatct
    19621 gctttgcttt ttaactttct accagagcgt acagttaaaa tagcatataa tacagtaaat
    19681 atggcagcta aaacatatga tatatttatg tttaagttaa atccaatttg agcattaaat
    19741 atataactga atataacaaa ggaataaaac ataccaggaa ttaagcttat aatataattc
    19801 ttttctttta ttataaggta aactgtaatc attgcaaatg caaatatggc aatagtttga
    19861 ttactccaag agaagtatct ccacaatata ttaaatccag aagcactaag ctttgcaaat
    19921 ataaggatgg ctatgacagg aataaatata cagattgaca atataacacg atgttttttt
    19981 tccttttggt cataatgaaa gtaatctgca agcattaatc ttaaagatct taaagctgta
    20041 tctccagaag ttattggtaa aacaattaca cctattatag ctatgatacc accaatagaa
    20101 ccaagtaaat ctcttgcaac caaacctatt acatcaggag aacctactaa ttccttaggt
    20161 attcctttat tgtaaatacc catagcagct gcagcccaaa tcattgcaat taaaccttct
    20221 aaaatcatca tattataaaa agtagttttt ccttcttttt catttgaaac tgatcttgct
    20281 ataagagtag cttgagttga gtggaatcca gaaacaatac cacaagcaac tgtaacaaag
    20341 aatgtaggaa taagaggaat accatcagga tgaatacctt tccaatttgc aagacttaaa
    20401 ttagctaaat cataaccttg tgtaaagatt ccaattccaa caccagcagc agataaaagt
    20461 agaatagctc caaatattgg atataccttt cctatgattt tatcaattgg aaatagtgtt
    20521 gcacacaagt aatatagaaa gataagtcca taaactatcc agacaactgg attatttata
    20581 gtcgatttca tatttaaaat ttgagtaaca actaaatccc caggtgtata aataaaaact
    20641 gcaccaacta aaagcattaa aagacaaagg aatatattat agacttgaaa tactttatta
    20701 cctaaaaatc tactgataag agaaggcatt tgtgctcctt tttctcttat actaatcatt
    20761 ccactcatat agtcatgcaa agctcctcca aatacacagc caataggtat tagtatgaat
    20821 gctattggac caaacaatat accttggata ggtcctagaa ttggtcctgt accagctata
    20881 ttaagcaact caattagact atttttccat tttttcattg ggacatagtc tactccatca
    20941 ctttgagcaa gtgcaggtgt ttttctgtca tctgggccaa atactttttc acaataagca
    21001 ccgtataaag cacctcctat tagtaatata gccaagccga ttaagaatgt tgtcataata
    21061 tcaccccatt tataaaattt tttggtcttt gtatacatat tatctaaatg tgtagattta
    21121 tattgaattt aattctggac aacctacaaa ttctataaag tattacaact aatataattt
    21181 cagaaataaa tacatataca taaccatgta cttagtatac aggtatttgg taatattttc
    21241 aatgttatag caataaagaa tatagacaaa attctgataa tattttacaa aaaaattaaa
    21301 aaatataaaa attagtttaa atagatggag aaaaaaggaa aatcaattga gaatataaat
    21361 gtctatatct taacaatatt aagaggttta aaattcttga agaaagaata gtcaagtgat
    21421 ataatgagtt tacaggtgta tatacactag tacatataga aagcggtgca attatggata
    21481 aagatttaac atatcaaatc aaagagctaa agaaagaaaa aaatgcaata atacttgccc
    21541 atttttatca accaccagaa atacaagaat tagcagatgc agttggtgac tcatattatt
    21601 taagtgagat tgctagagat tgtaaagaag aagtggttgt attttgtgga gtaaggttta
    21661 tgggagaaag tgctaagata ttatcacctg aaaaaactgt attgatgcca gtaagtaacg
    21721 ctgggtgtgc tatggcagat atggtagatg aagaaggcgt gataaaatta aaacaacagt
    21781 atccaaatgc tttggttgta tgttatataa actctacagc taaagttaag gcacattgtg
    21841 atgtttctgt tacatcttct agtgcaatta aaatactaga aaacatagat aataaagaaa
    21901 ttatattcct tccagataaa aatttaggag gatatattgc agaacaattt ccagataaaa
    21961 actttatatt ttgggacgga tattgtaaat accataacaa tataagagct gaagaaataa
    22021 ttgagttaaa agataagtat aaaaatgcag aagtattagt acatccagaa tgtaaaaaag
    22081 aaataagaga tttaggagat tatgtaggta gtacatcagg aataataaaa tatgctacta
    22141 atagtaaaaa caaagacttt ataatagcaa ctgaagaagg tatattacat gaattaaaga
    22201 aaaataatcc taataaaaac ttttatattc ctggtggaaa aatactatgt acagatatga
    22261 aaaagacaac attagaaaat ttatatagta cacttaaaaa tatggaaaat gaagttattg
    22321 tagaagatga aatcatggaa aaggctttaa attcactgct taacatgcac aaattagcag
    22381 aaggatagag ggatagactt atgaatttag aacaagatgt tttaatagtt ggaagtggag
    22441 tttcaggatt atactgtgca ttaaatttag ataaaagttt aaatgtgcta gtagtatcga
    22501 aatcaacaat tgaaaacaac aatacatatc ttgcacaagg tggaatttca actgctagaa
    22561 atattgatga tataaaatcc tttgtagaag atacaatgaa ggctgggcag tataaaaata
    22621 gagttgaagc agttcaagta cttgcagatg aatctataga aaatgtaggg cagatagtag
    22681 aatatggtat gcctttagac aaagaaaatg gagagataga ttacacaaga gaaggagcac
    22741 atagtgtaaa tcgaatagtt catagtaagg ataatactgg agaagttgtt tttaaaactt
    22801 tattaaaaga agctaaaact agagaaaata taactcttat agaagatgcc tatcttttag
    22861 atatattaaa agatggcaat aaatgtatag gagctagaat ttttaaatct aaaaaagaga
    22921 ttcatgtttt ttcaaaaata gtagttttag ctacaggtgg aattggtggg ctttttaaaa
    22981 attctactaa tcaaagacat cttacaggtg atggaattgc aatagcttta agaaataata
    23041 taaaaataga aaatttagaa tatattcaaa tacatcctac agccttttat gaagaaaata
    23101 atgaaggtag gagaatgctt atatctgaat cattaagagg agagggagct aaacttttaa
    23161 ataaaaataa agaaagattt gtggatgaac ttcttcctag agatgtagtt tcaaaagcta
    23221 tttttgagca aatggaaaaa gataaattac cttatgtgta tttagatgca acacatttag
    23281 atagtgaata tttaattaat agattttctt ttatttataa tgaatgttta gctagaggaa
    23341 cagacataac gaaagaatgt ataaaggtat ctcctgcaca acattatttt atgggcggga
    23401 tacatgtaga tttggattca aaaacatcta tggaaaattt atatgcagtt ggtgaaatta
    23461 gttgtacagg tgttcatgga gccaatagat tggcaagtaa ttcattactt gaagggcttg
    23521 tattttcaaa aagagcagct aaaaatataa actcagttat agataatgta aaagttaagt
    23581 ttatagatgt accagatatg gatatagata tagaacaggt aaaaaaagaa aataaaatat
    23641 tagtcataaa agaaattgaa agaacaagtg aggattttgg cgatgaatta tttgattatt
    23701 gatagaatga tacaggatgc tttgatagaa gatgttccaa gtgaagatat aacaactaat
    23761 tctattgtgg atgaaaattc taaatctact gtagatttaa tttgtaaaca agatggtata
    23821 atagctggct taggtgtatt taagagagtt tttgagatac ttggtgatgt agatgtaaaa
    23881 ttatataaaa atgatggtga tagagtaaag aatcgtgaaa agatagctgt attaacaggt
    23941 agtactagaa acttacttgt aggagagcgt gtagcattga attatttaca gagaatgagt
    24001 ggtatagcta ctattacaag taggtatgtt gaaaagcttg aaggtacaaa tacaaaatta
    24061 ttagatagta gaaaaacgat acctaatctt agaatattag ataagtattc tgttaaagta
    24121 ggtggaggat gcaatcatag atttaattta tctgatggaa tattgcttaa agacaaccat
    24181 attggggctg ctggtagtgt taagaaggca gttgaattag ctagaaaaaa tacttctttt
    24241 gttagaaaga tagaagtgga agtggaaact ttagacatgg taaaagaagc aatagaagct
    24301 aatgcagata taataatgtt ggataatatg agtttggaaa tggcaaaaca agcagtagat
    24361 attataaatg gaagagccat tgtagaattt tccggaaatg taaatttaga tactatagaa
    24421 gatataggaa aaattggtgt ggatgttgtt tctgtaggag cgcttacaca ttctgttaaa
    24481 gctctcgata taagtatgaa gaatttgaga aatatataaa ttttatattg aaataaactt
    24541 aattaaaata gactccttaa atttgtttta taaatttaag gagtctattt tttattcatt
    24601 aaaattaata aaaatagaaa aaaatcaatc aataatttaa taaaaataga taaatactat
    24661 atacactttt aataatatag ttaatatcta ttttacagat aaataagaat aaacttataa
    24721 tataattgta tctgtaaaat tttgtaaaat ataaaaagta tagggggaat aaaaatgaaa
    24781 gtagcttata tttttagtag tactaataca cataaaattc tagacaaaat gataatacca
    24841 caacttgaac aaggaactca tggagtagac gttctaggga tgtttttctt tatggataat
    24901 accttatttt tatctaaagg taatcctgta ggcgagagac taagtaaatt acatgaaaaa
    24961 actgatatga taataatggc ttgtgaccaa tgtgctatag aaagaggtat agagaataat
    25021 ttggttgatg gagctacaat tggatgtttc ccaaacctat atgcttgttt aggcagtgca
    25081 ggtgtggacc aagttataac gttataaatt agtataggat acaatccata gaaaaattta
    25141 ttttctgtgg attattttaa gtaaaagata aattgtttaa agtaggggat taacaaatgt
    25201 ctaaaatttt agttgaattt ataaacactt gaccatcttg tgatgaacat ggagtgttta
    25261 taaaaaaaat gtcagaaaaa tatgctgata aattggaaat aaaattatat caagcaggga
    25321 aagatttttc gtatataaaa aaatatggga ttataacaaa aggaacttta ataataaatc
    25381 agaaaaagaa atatgataga ttaaataaag atacaattga aagggcaata gtagaagcta
    25441 taaacaataa ttaaaagttt aaaaattagg agtatcaaaa atgaatatat tagaaattgt
    25501 tttaaatgga gttaaagata ctatagtagc ttctatagtt acactgaatg gagcatcagg
    25561 atggcttatg ataagtttta tattggcagg tgtacttcac aatgttttaa ctccaagaag
    25621 gtttcaaaaa caacttggaa ataagaaatt ttcttcttta attaaaagta ctatctcagg
    25681 aatgttttta ccaatatgta gttgtggagt aataccactt ggaataagta tgtattattc
    25741 aggagcatat ttaggaccag tacttgcttt tatgacatca actccaataa taaatccaat
    25801 agctgtagtt ctttgtctag gtcttttagg tcctaaagtt acaatcattt atctaatagc
    25861 tggtttttta gtaccatttt tagtaggatt tttaggaaat tgttttggaa aagaagaatt
    25921 atcacttcat aatgattcag aggaagaagt aatagaatta gaagaagaaa aaagaagttt
    25981 tatgggaaat atgattgatg gtcttaaatg gtcttttggt gaattagccc ttactattag
    26041 taaatatgta gtaatgggta tggtttttgc aggatttata acaactattt ttccaaatag
    26101 tataattcaa aaatatttag gaaatccagg tttgctatct cttggaagta tagcaatact
    26161 tgcatgtgtt atgtatgtat gtgctgtagg acatataccg tttatagcag cccttgttgc
    26221 aagtggagca tctcctggta tagcgataac atttctaata gctggagcag ctacaaatct
    26281 accagaactt ataagtatgt ttaaacttat tggaaaaaga actgttatta tatattctgt
    26341 aactttgaca ctgtcttcaa taataattgg atatataaca aatttacttt tagtgaataa
    26401 taactcagta aatttagatg caacaaattc ttcaatacag ttggcaaata aactaatgtt
    26461 aaatatacct gatagttttg agtatgtatg tactatttta attattttat ttgcatttaa
    26521 atcaataata ccaaagatga aagaggtcat aggaaatcat gcttaaaaag atagttataa
    26581 tatcctgtat agtagttgtg ttaatcattt tatcaaaaat agttgatgat aacatcaaag
    26641 aagatgcttc tattccaaat gtgaataaag aaacattaga atattttaga aaaaattata
    26701 aagaagacat aataacttgt gctgaagaag acttaaataa tgatggaaaa aaagatttag
    26761 tagttatata taaaaaaagc aataattcaa atgaaatggt agtcgtagtt tctgataaaa
    26821 atagccacta tataacaaag ccaataccag cacctataga gaatcaaacg ataaccttta
    26881 aaaatattga tgataaagca ccaatagaag taattgtatc agggtctaaa aatggtaatg
    26941 taggatatgc tatttacaga gtagagggta aaaaatttgt tgatttattt ggcgaagata
    27001 tggacaaatg ttgctaaaaa taaaaataat acataaaggt gggaaattta tatgaaatta
    27061 tctaagaaga ttcaagcatt aatcttattt ggtgtaatgg gtacatctat attaacagga
    27121 tgtagtagta aaccaaaaga aaaagaagaa gcaaaaactg gaaaagatga ttatacaata
    27181 acagtaggtt attataattg tgaccatatg actgcgggac cagtagcaga atcggcagga
    27241 atatataaag atttaggtct taatgtaaaa actgttggaa atggtaaagt tcctgaagct
    27301 atggcagcag gaaaaatgga tgcaggttac ataggaacta aggggttagt tggagcaata
    27361 cctaagggtt cgccaataac aatagcagct aacaaccata ctggtggttc tgaatattta
    27421 atagtttcaa aagatataaa agaacctaaa gatttaatag gtaaaaaaat tgcaacagat
    27481 atgagtgatt tcttatggac aagtgattat ggtccagaaa caggtctacc aactgaccca
    27541 tcaaaatatg aagttgtaaa tatggattca gacaaagata aatatttagc tcttaaaact
    27601 gggaaaattc aagcttttac atcttgtgac ccttggggtt ctgtagctga aaatgacggt
    27661 gcaggaaaaa taatagcatc tactcaatat aaagaaaaag ctaatggcaa agaatataac
    27721 tgttgttcat tctcattaaa caaaaacttt ataaaagaac atccagattt agcaaaaaaa
    27781 ttggttctag cacatacaaa agcaatagaa tatatatata caaatccagc tgaagcagct
    27841 aaaatatttg caaaatatta taatgtagaa gaagaagttg cacttagaac tatctataag
    27901 aagactgttg gagaaggtag aactcttact tggaaagtta caggtgaaga atataaaaat
    27961 aatttacaaa tgtataaaga tttaaaggct ttagatgata tacctaaata tgaagatacg
    28021 attgatacaa gcttattgga ctcttgtgga gctgatgatt ttgacaaatt tataaaagaa
    28081 aaagttaacc cagagtttcc tgaaggtatg agttatgaag actggaaagc aaaggtatta
    28141 taaataaatg gagaatatga aaaatataat aatacttttt atcattattt tgggaagtat
    28201 atttataata ttttttaatt ttgaagataa agaagaaata aaaggaaaat taagaatagg
    28261 ggtatctgat gatacctctg gttttgtaat aaattatatg gtaaatagag actactttga
    28321 aaatataaaa gtaagtgata taatggatat ttttacaatt aatgattgct gagcaagtac
    28381 aatgcagtgg gctctgagtt cagaggaaat agatatagca atcatatgta aagatgctgc
    28441 aaaacaatat gtaaatgtaa atagtggatt tgaaattgtt ggtactgtag ttcaaaattc
    28501 tgatatattt ctattaagtg ataataatcc tgaaaagata ggagtcattc aaaatagaga
    28561 ttaccagtca gaattggtta aaaaatacta caagaatgtg gaagtagctc ccttattagg
    28621 gactgcacta gcatatggat tagaaagtaa tttagtagat ggtgttgtaa tagattgcat
    28681 aaagtcaatt gggttagagg gtaaaagaaa atctacaact aaattaggag attatgatac
    28741 ctatgtatta gttgtaaata aagagtttaa aaaaagtaaa gcatatagtg attttataac
    28801 actttacaat aaaagtgttg atgctttaaa gaataaagct acatttaaga aagtattaaa
    28861 agagtacagg aatataagaa atatagatat ggaggaaata agcaattgga aattaaaatt
    28921 tctaaagata aaagcgatag attaactaag aaaaaaactg atggtagtat ggatatttta
    28981 tataaaataa tactattagt tattatattt ttagtctggc attttgcagc aaaagatata
    29041 gggagttctt tgttacttcc aatgccagta gatgtaataa aaggtttctt tttctgtgtg
    29101 acagatgcag agacagtcac taatcttttt ataacattgc agagagtact taaaggtttt
    29161 atgtatgcat tattgtttgg gcttccaata ggatttatta tgggattttc taaaactttt
    29221 gaaagggttt tatctccagt agtagattct gtaaggcaag ttccaataat ggcatgggtt
    29281 cctcttacta tagtatggtt tggtatagga gatggtccaa caatattttt aatagctttt
    29341 tcaggggttt tccctataat tttaaatact attcaaggag taagagcaat atcaaaggat
    29401 tattataatg cagctagaag tatgggagca tcgccaattg tgatatttac aaatgtaatt
    29461 gttcccgcat ctcttccaga tatactaact ggttcaagga ttgctataag tacaggatgg
    29521 atgtctgtta tctgagccga gtttatagct acgagtgccg gtctcggtta ctcaatggta
    29581 caagctcaaa cattaatgca tacagatgta ttaattgggc ttatgatatt tgcagcttta
    29641 ataggattta taatagatag ggtattaaag tttataaata aaatcctttg tagatggagg
    29701 tttgcagatt aatgaagtta agtgtaagag atataaataa gacatttgta aataatagag
    29761 tacatacaaa ggttttagaa gatataagta tggatataga tgatggacaa tttgtctgtc
    29821 ttttaggacc atcaggttgt ggaaaaacaa ctttacttac tataataggt gggtttcaaa
    29881 aatcagaacg tggagatgtt tttataaatg ataaaagagt taaaaaaccg ggcatagata
    29941 gagcttttat atttcaaaat tatgctttat ttccttggaa aactataaga ggtaatgttc
    30001 tatttcctat gaaacagcag aaaataccaa aagaaaaaag agaagaaatg ctagaagaac
    30061 ttttggttat gtcagacttg aaaggaaaag aaaaactgtt tccgcatcaa ttatctggag
    30121 gaatgaaaca aagagtggcc atgataagag ctttagcatg taatccagag gtattactaa
    30181 tggatgaacc attaggagct gtagattttc aaatgagaca aaatttacag gaagagctag
    30241 aacgaatatg gataaagaag aaaataactg cattaatggt aactcatgat gttgatgaag
    30301 ctgtatatat gagtgataga gttatagtaa tgtcgagaga taaaggaaga attattgatg
    30361 atataaatat agatattcca agacctagaa ttagaggaag ccaaaaatat gaagagtaca
    30421 aaaacaaatt aactgatact ttatcaaagt gttatgaagt ttaaagagga gtagacatga
    30481 gtcaaataga aaaatacaat agtttggata atacacaaca gaaaataaga atctctatta
    30541 tggatatgat aatagataaa ggttctagtg ttacacttca agaagtgact gaatatgtaa
    30601 gtaaaaagtt gaatatagaa aaagaatata tagaaaggac actacaatat tttatttata
    30661 aaaatataat ggttgttgat gggaatagta taaattttat atatcctgta tcagcattgc
    30721 caacaaatca taaagtaact ttgagagata atagaagttt cagtgcaatg tgtgctatag
    30781 atgctatagg aacctcttgc acattcaacc aagatataaa aataaactct atttgtagtg
    30841 ctacaggtaa ggaaatagaa gtaattataa aaaatgaaaa gattgagtat gtgaataatc
    30901 caaacttaag agtgcttcat ataaatcttg ataagcatct aaattgggct gctagttgct
    30961 gaaatataat gaatttcttc tggacgaaga gtgattttga tgcttggaca aacgaagcgg
    31021 gattatctga tgatgaagat atatattgtt tagatattaa tgaggcaata gtagaatcat
    31081 ataaaatatt taaactcaaa caaaaggtac tatcatagat atacatatat gaaaaactaa
    31141 aaatgacctt tagataaaaa tgataggtaa tatccatact atataaatta gtatagagta
    31201 gggatgtgaa aagtatttaa atctaaaggt atatttttag tttttttaga tgattagata
    31261 gccttattaa gtatatattt atttataacc tttgcaactc cattttcatt attagtatca
    31321 gtaacataat ctgcaatttc ctttatacta ttacgtgcat tccccatagc cacaccaaga
    31381 cctgcatact ttatcatatg ttggtcattt tcttcatcgc caacagcaat agcattttct
    31441 ataggtatat ttagattcgt acataatgtt ttaagagcag ttcctttatt agtagtttta
    31501 cctaaaaatt ctagataaaa aggagcagac cttactatag tatatttatt gaataaattt
    31561 ttaggtagtt gctgtatgat ttcttccaat ctttctggtt catcaactat cataatttta
    31621 cagattttaa tagattcatc aattttatct ttttgtatat atttgacttc tatattattt
    31681 aatttcgctt catgaagtgt atatttgcca ggcgtttcat ttggtgtaat taaatttgtc
    31741 attgtattta tgtgtatttt agtcttatat tttttacaaa cgttattata tataaaatca
    31801 aaatcatcta aactcatttc tacatcatgt atgattttct cacttttaac ttcttgaata
    31861 attgctccat taaagcatat aacataatca ccttcatctg ttaaatttag ttgatttaga
    31921 agttctttta caccaggaag aggtctgcct gttgttataa ctacttttgc cccagccctt
    31981 tttgcctcct gaatggactc aaagacttca ggagtaatcc ttttttgtgt atttaatatt
    32041 gttccatcaa tgtctagtgc gattaatttg tacatatcaa cctccaataa attatatact
    32101 caaatttcct gtgaaaacgt tgacacagct agtgttgaac aaagtttcag tgggaaatat
    32161 ggtttgtttt gtatactatt atatcaagag ataagtaatt tagaaagttt aataatatta
    32221 attttttgat aaaaatatta cttatgtgag atgagaatgt aaaaactttg taatcaataa
    32281 ttccaaaatt cttaattaca aataatggta tcgtttgcat tgttaatata aataaatgta
    32341 tattatatag ttagaagttt gaataaatag actgatggta atttgttata ttagttagtt
    32401 ttgtgttata aaatattgta attccattaa tcatatgtta ttattcaaca tgattataat
    32461 ataaagaata tttgatatat ataataattt ataaaagagt attacatagt acatataaat
    32521 ctatatttat aaatacaata aagtaaagtt gtaaaggagt gctttaaatg aagttttcag
    32581 agtttaagta tgaaagaccc aattataata gtatgaaaaa agagtttttg agttgtgtag
    32641 aagatataaa taattctcgt aattataaag agcaacaaaa aaatatacat aagattaatt
    32701 tattaagaaa taaaatagaa acgttgtcaa atatagcttc gataagatat agtacagaca
    32761 catttaataa attctacaaa gaagaaaaaa attattggga tgaatatatg cctttgtatg
    32821 aagaattaaa ttcatatttt tataatgcca ttgtaaattc aaaatttaaa tatgatttga
    32881 taaaagagtt tggagagcaa tttttcacaa tagtagagta ttctttaaaa agtttttcca
    32941 aagaaataat aagtgaattg caggaagaaa ataaattgtg ttcagagtat actagacttt
    33001 tagcttcagc tgagataatg tttgatggga aaataagaaa tttatctggt atggggaaat
    33061 tcatgtattc taagaataga aaaacaagag aattagcaaa caaagcatat tataatttct
    33121 ttgaagaaaa tgaaactaaa tttgatgata tatttgataa attggttaaa cttagagata
    33181 agatgtctaa aaaattaggt tttgaagatt ttgtagagct tgggtacatt agaatgatga
    33241 gaagtgacta cagagaatat atgataaaaa atgttagaaa acaagtacta aaatatgttg
    33301 ttccaatggc aaatgagctt tatgaaaaac aagctaaaag aattggtcta gaatatttaa
    33361 gttacataga tgaaggcgtg gaatttttaa caggaaatgc aagtttaaag ggagattcaa
    33421 gatacataat taaaaatggt aaaaggatgt attctgaact gtcaaaagaa acaaatgaat
    33481 tttttgattt tatgttagaa aatgaactta tggatttaga aacgaagaaa ggaaaaggag
    33541 ctggagggta ttgtacttat ataccagatt ataagtcacc attcattttt tctaatttta
    33601 atcaaactgc tgatgatata gatgtattaa cacatgaggc aggacatgca tttcagttgt
    33661 atatgtctag atggattgat atgccagaaa ttaattttcc aacgcttgat agttgtgaaa
    33721 tccattcaat gagtatggaa tttataacat ggccgtggat ggatttattt tttaaagaag
    33781 atacagataa gtacaaattc actcacttat catcttcaat taaatttata ccttatggag
    33841 taattgttga tgaatttcaa cactatattt acaaaaatcc taatgtagat aagtcaaaac
    33901 gaaaagaaat ctggagattt ttagagaaaa aatacttacc tcatagaaaa tatggtgata
    33961 attctttttt agagagaggt tgttggtggt ttaaacaagg acatatattt aaaaatccgt
    34021 tttattatat agattatgta ttagctcaaa tttgtgcgtt acaattttgg aaaaaaatga
    34081 ttcaagatag agatgcagga tggaaagatt atataaatat ctgtaaagtt ggaggaacaa
    34141 aatcattttt agatattgtg agtatgggaa atttgtattc tccatttgat gatggctgta
    34201 ttgagtctat tataggtgat gttaaatctt ggtttgatga aataaatgac agcaaattat
    34261 aaaaaattat gagaatttta ttgtataaaa gtaaaaaatt aaaaaatata taaaaagtga
    34321 ttatttgttg tgaaaatagt cttgttttta actaaattga taaaataaac taaaaaagat
    34381 ttgtacttaa aaaaaaaaga aaatacaaga aatatgcaat atcaacattt ggaaaaaaaa
    34441 gtaaaaatgt atacaaaata catataaaaa aactattgca aattttatag aatgtttata
    34501 tacttaagtc aacagagata gaaaaaagct atttatagaa aacaaaggat aaattgctta
    34561 attttaaatt ttctgaaaaa aataataatc cggatgaaga gtatgggaga ctgctttata
    34621 aaaagcgacc gaagggacaa gtttaatctt acttacccaa aagattagat gaaattctca
    34681 ggtaaaggtg actgtacttg gacggggctc tgaaaatgat tttaacaggg aagacaaatt
    34741 gggagatttg tttttcctgt tttttattta tcaagtaata aataaaagtt tgaaagagag
    34801 taaaaaaatg ataataatta cgaataatcc aaaagtaaaa gaagaagttc aaggcagaga
    34861 agttttgttt aaagacacaa cttatattgg aatcttagaa gcaagtagag atttgataca
    34921 tgaaggttat gaacttttat ctcatccact ctatgggagt gtaaagccaa atgaaacacc
    34981 ttacaggact gttatactaa agaaaggaaa tcgtttggat ataaactctt taaccttaat
    35041 tgaagaagca atcatcactg caagtaaatt tcagaataat aaaaagactc caaaatggac
    35101 agaaagtgtt caagatgatt ttagagtaat tgattatgat atattttata atacaataca
    35161 aagaatgcag tatgaataaa aaaattgtta aaaagttatt aaaacagcct aaatattaaa
    35221 tctaatttaa tattaaataa aatgagaaat ttaattgggg aggataagtc aaaatggaaa
    35281 atgtgtatga cttagtaata attggttcag gaccagcagg gcttgcagca ggtctttatg
    35341 gagcaagagc aaagttaaaa actctaatcc tagaaaaaga caagactggt ggacaaattg
    35401 taataacaca tgaaattgca aattatccag ggtcagttcc aaatgcaaca ggaccaagtt
    35461 taatagctag aatggttgaa cagtgtaaag agtttggagc agaaatgcta agagacaaca
    35521 ttgttgatac tgagctagat ggagatataa aagttttaaa aggggaaaag gcagaatata
    35581 gagcaaaagc agtaataata ggaacaggag caacaccaag aaagattggt tgtcctggag
    35641 aaaaagaact tactggtaaa ggggtttcat attgtgcaac ttgtgatgca gatttctttg
    35701 aagattttga agtatttgtt gtaggtggag gagacagtgc attagaagaa gcaatgtatt
    35761 taactaaatt tgctagaaaa gtaacaattg tacatagaag acaaggattt agatgtgcta
    35821 agagtgttga agaaaaagca aaagctaatc caaagataga gtttttatta gatacagtaa
    35881 ttgaagaaat caaaggtgat ggaatattag aatcagttgt atttaaaaat aaagttactg
    35941 gagaaactca tgaatacttt gcagatgaag aagatggaac aatgggagta tttgtgttcg
    36001 ttggattaga tgctcaaact gatttattca aaggtaaagt tgatatggat gaaaaaggtt
    36061 acataataac tgatgaagat atgagaacta acataccagg cgtatttgca gcaggagatt
    36121 gtagatctaa gactctaaga caggttgtaa ctgcaactaa tgatggagca attgcatcta
    36181 tagtagctga gaaatacatt gatgaaaaat ttgggaacta gaatctgtaa attaagaaat
    36241 agtgtctgta aatttaatga ataatattta aaattaaaat taatatttaa aataaaaata
    36301 aaaatttcaa gggggaataa gaaaatgtta gatttagata aggctacatt tgaagaagaa
    36361 gttttaaatg cagaaggttt tgtatttgtt gatttttgga gtgaaggttg tgagccttgt
    36421 aaagctttaa tgccagatgt tcataaatta gctgagactt atggggataa aataaaattc
    36481 tgtaagatgg atacaacaaa agctagaaga ttagctataa aacaaaaagt attaggtctt
    36541 ccaactatgg ctatatacaa agatggagaa aaagtagacg aagttactaa ggatgatgct
    36601 acagttccta atatagaaaa catgattaaa aaatatcttt aatatgttga taaa
//
LOCUS       DAYAPA010000028        35997 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.028, whole genome shotgun sequence.
ACCESSION   DAYAPA010000028 DAYAPA010000000
VERSION     DAYAPA010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 35997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35997
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.028"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(316..813)
                     /locus_tag="ACGI59_001291"
     CDS             complement(316..813)
                     /locus_tag="ACGI59_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFG9739093.1"
                     /translation="MNYEIVEINEKIVIGISKETTNKDGQAVNDIGELWKKFMGKGIY
                     NAIKGKKNDKTIGLYTDYQGDFTSPYSFVACCEVNSNSDKEKNLEELNTNNTVGESII
                     SKVIPAGKYAKFVIVGGQKEVGDFWFEFWQMDFDRTYISDFEEYQCNTFDTKKQEIHI
                     YIGIK"
     gene            complement(988..1899)
                     /locus_tag="ACGI59_001292"
     CDS             complement(988..1899)
                     /locus_tag="ACGI59_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFG9739094.1"
                     /translation="MKINRLTEIIVILLNKKLVTAKELADRFEVSTRTIYRDIETLSM
                     SGVPVYMTKGKGGGISLIEEYSIDKAILSKKDKESLIVALKTLQATKYPEINSVVNKI
                     GSIFGEQNFSNWIEIDFTEWGSNFNEDDKFTKIKEAILRRNTINFNYVNSLSSQTNRT
                     VEPLKLMYKSKTWYLYGFCKLKDDFRIFRISRIRNLSIKDEVFSRKIIEEVCLNDSKV
                     IKENTITLKLRFKEKMLFRVFDDFNKDLITKNEDDTYDVITEFPIGEWIYGYILSFGD
                     NVEVLEPKDVRDNVITRLRELSKIYSL"
     gene            2823..3674
                     /locus_tag="ACGI59_001293"
     CDS             2823..3674
                     /locus_tag="ACGI59_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3100 domain-containing protein"
                     /protein_id="HFG9739095.1"
                     /translation="MEINKKHFIQVFLTCLILVVICEFIGQRTIAIGKASIVLFPMLY
                     AVIIGLIITPDLLGKKIKALKKVVGEKEINIAGEVVGIALVMLGIKYGTTVGPNIDKI
                     IQAGPAFIAQEFGHILAPIVALPLALMFGMKREAVGATASISREPSLGVISEKYGINS
                     PEGSGVLGTYLMGTVLGTIYFSVLGSISIYSGLHPYALGMACGVGSGSMMTAAAGSLS
                     TMVPPEMADTILAYAATSNMMSSITGIIFLVFVSLPFTNFMYKILEPKFSRNKKTKEI
                     KSTEGEC"
     gene            3678..4148
                     /locus_tag="ACGI59_001294"
     CDS             3678..4148
                     /locus_tag="ACGI59_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9739096.1"
                     /translation="MFANLNLKKSSYQLLVMSVIGVIILIGQRISVGTSIVTALPGMV
                     MLILAAMAAMIIKDLFPKSIFPAFGFATIIGLLLSMPYSPTSEVFLTNTNNINFMAIT
                     TPLLAFAGISVGNKIEALKEMSWKIVIISLIVFTTIFFACASIAHIVLSIQGKI"
     gene            4161..5471
                     /locus_tag="ACGI59_001295"
     CDS             4161..5471
                     /locus_tag="ACGI59_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HFG9739097.1"
                     /translation="MNKEQLKSLVIKTIDENREKILKIGQGIYKNPEYGYKEFKTTEA
                     VSNFFEQELNLSVEKNIAVTGCKADANSSKKGPHISILGELDGISCKEHKDANEIGAS
                     HTCGHNIQIAGMLGAAVGLVKSGVLEHLDGKVSFMATPAEEFIELEYREQLKKEGKIT
                     YFGGKQELIKRGYFDDIDISMMFHASDLGEDKALVGPESNGFVGKKVKFIGKESHAGS
                     APHDGINALNAAMLAINNVNALRETFKESERVRFHPIITKGGDIVNVVPADVHMESYV
                     RARTINGMIDANERINKALMAGGMAVGADVEITEIPGYLPILRYKSLDNLFKNNLEDL
                     GLKGKVIDGGDFTGSFDFGDVSHIMPTLHPMIGGVKGALHTRDFEIVDEDLAYIIPAK
                     AMALTVVDLLFDNAKDAKDILDTFTPVMTKEEYLTFLEKHDKTL"
     gene            5683..6429
                     /locus_tag="ACGI59_001296"
     CDS             5683..6429
                     /locus_tag="ACGI59_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFG9739098.1"
                     /translation="MFNAGNRIINTWLYPIQDGFVLIDTGYENGFKHFKKRISKFNIK
                     IKDIRYIFLTHAHDDHVGFLNQILDESPNTKIVMHDKALEILYKGQNSFIGGCTSRTA
                     FLFCQIMKLFGKGEHSFSPLKQEFEKRCILVSDKNRKEIEKELNGKIIDTPGHTDDSI
                     SLLIDEGVLFCGDSAMNGFPSTHKITIFAESKLEFIQSWKTIINTKPKMIYPGHGKPF
                     EYSKLEQNLIYVNKINLYPLHNSKDIHRFL"
     gene            6791..8332
                     /locus_tag="ACGI59_001297"
     CDS             6791..8332
                     /locus_tag="ACGI59_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HFG9739099.1"
                     /translation="MNDNVVKKKNFFDRTLNRIETVGNKLPDPVTIFLGLCVLLLILS
                     SLVGSMGISVVHPGTGETITAVNLLTVEQLQILLGNIVSNFQGFAPLGLVLVTMIGAG
                     VCDKTGLMTATIKASVSKIPETRVTLVVMTIGMLANIASDAGTILFPPLAALVYLGVG
                     RHPLIGLFSGYAAVCLGFAANIMISVNDILAASFTVPAAQMIDSGFQANATMNLFFMI
                     ASTFVLILLATWVTEKIVAPRFGKYEGNAELDVDGSLSDVEKKGLKKASISILIYVAI
                     IVALSVIGKKPFLADPETGDLLSNNAPLMKGMVPIIALAFFIPGVVYGKTIGKIKKDK
                     DVVSIMASAMSDMGGYIILAFAASQFLQLFTNSNLGLILAVKGGEFLKSAGINGPLLL
                     IGFIILSCFINLFIGSASAKWAILAPIFVPMFMMVGYNPALTQMAYRIGDCITNPLSP
                     LFPYFPIILAFTRKYDKDAGMGTVIANMIPYSISFLIVWTILLILFMVFNIPLGPGIL
                     PSYSM"
     gene            8367..9473
                     /locus_tag="ACGI59_001298"
     CDS             8367..9473
                     /locus_tag="ACGI59_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HFG9739100.1"
                     /translation="MLDKNRLINNFMDMVRIDSPSNQELEMSKWLVNYLKERNIDAII
                     DDAGEKYGGNTGNVIAYIKGEEGSRPLCLCAHMDQVQPCLGVKPILDGNVVRSDGTTT
                     LGADDKAGIAAILEALEHVITEKIPHRDIYLCFTICEEAGMHGVKNFNPDNLPCKDMV
                     ILDSGGAIGSIAYKAPAQQSIKISFQGKKAHAGIEPEKGLNAILVASHAISNMHIGRI
                     DSLTTSNIGEIEGGGATNIVTDKVTLTAEIRSHIPETLEYELNHMEKCCKDAASKFNT
                     TYTFEHNMSYPSFELSRDSHVFKLSEEAIRQVGVVPNPMVIGGGSDANILANLGYNCA
                     ILSLGMYDVHTVNEYVNIDELYDTAKIVYHMIKL"
     gene            complement(9852..11138)
                     /locus_tag="ACGI59_001299"
     CDS             complement(9852..11138)
                     /locus_tag="ACGI59_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFG9739101.1"
                     /translation="MIVGIIGPSDSGFKIKDDLKQIDSDLKTKIYVREKVVDTIEVIS
                     KCEDECDAIIFTGCAVYEFIKSKYEISKPHSFVPRSGTSIMKAFWAIKSANIKLDKFS
                     IDVVDRYVVEDALKEFDIKATSVFCNPFSLEVGESELVEWHIKLFEENKTDIMLTGFG
                     AVYNELKERGYPVFRLQATIQLIKESYDKVKSEYALSKARFSQIAVEILSLIDYKEKI
                     DNYYSDMIKKSDMDKLVVNYVRSIQGSLFLLGRNDYVVFVHKGVADNEYNYDKLFNLK
                     REIKDMGFSLSIGIGTGVTAYQAENNAHKALRHSIDSKEIGIYLVDEDENIRGPLASE
                     NELNYSLMLSDEKVIEISKKTGMSCESVAKIIAINENRKSKIYDSKELAEYLNVSERS
                     ARRILNKIVNAGLGRICAKETSIGGGRPKNITEILF"
     gene            complement(11261..14359)
                     /locus_tag="ACGI59_001300"
     CDS             complement(11261..14359)
                     /locus_tag="ACGI59_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFG9739102.1"
                     /translation="MDVINLIDELDEIVYVSSLDTYELLYINEIGKKAMGLNDISHLK
                     CYKALQGKDSPCEFCTNSILKEDEYYIWENTNTRTNRHFILKDKLIKWEGKHARLEIA
                     LDITEKEDISKSIAEKLEIKELLVGCIKHLITESNFSDAVNLVLTNIGKFHKADRAYV
                     FEVSEDRKKVTNTYEWCNEGVDEQKQALTNLDINELKNWFLLFEQKGFVIIDDIENIK
                     EEQPIEYEILKPQKIESLMTSSLKQDDKVVGFIGVDNPHKAVGDTSLLTSLSYFLLTE
                     MKKRKVENKLNYMSYYDFLTGAYNRNKYIKYIDNFQKGKVKSIGVVFVDINGLKDIND
                     NFGHANGDSAIVSACVIIKKYFHSYNVYRIGGDEFVVLCEDISKQLFEELVSWLSEEF
                     KDEMIYSVAVGSIWSDDNIDIHKLIKVADERMYQDKKRYYNKNKLRNNEMKVISDLAD
                     STNNILFKHKELVDKVYFDDLMIKGYSKAYEKLAYNTYDIILSICIDTNLITPIYQSE
                     SNIFSFPIKENLEKTFSYIKKHLIHPDDIILFNDNHLLKSLDSLNNSKQDDLELNFEY
                     RRLGIDKKYYWALIYMLKISSKNKLGEKQTHILVAIKNIDYLMQLHKNQKDTLTGLYN
                     YESFCEQISLMLKRYPDKRYAIIMMDIDKFKVINDIYGVRGGDKALIYVAELIQKNIG
                     SNGICCRMYADVFCIFYESYSDRDIHKVIYKIKNSLNRKKFEYMLVPCFGVYRILDNH
                     IPITNACEMAGYAHKKVKKQSINNYIFYDDTIRNDALEEKQMEREMEYALENGQFHVY
                     LQPKYNLNANKIVGAEALVRWVHPEKGIIPPIKFISLFEKNGFIIRLDMYVWEQVCSL
                     IRKWIDLGEEPVPISVNVSRLHLHNPHFKEIVLSLINKYSIPKSFLELELTESLFIKN
                     IKLFTKIIADLRMDGIVLNMDDFGSAYSSLNMLKNINIDTLKIDCGFFNEEGITDREK
                     IVLKHTIAMAKDLDMDVVAEGVETKEQAEFLVTLDCVVIQGYYYCKPIPSADFEKLLY
                     QK"
     gene            15085..15243
                     /locus_tag="ACGI59_001301"
     CDS             15085..15243
                     /locus_tag="ACGI59_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9739103.1"
                     /translation="MKIFSFLLFVIGICLCIAGFIGHYPVFVGTTLSFLAIAMIAVRQ
                     VKTKKKMK"
     gene            complement(15530..16234)
                     /locus_tag="ACGI59_001302"
     CDS             complement(15530..16234)
                     /locus_tag="ACGI59_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFG9739104.1"
                     /translation="MEIKPLVLIVEDDKPICKFIKVSLETQNYRCVETDNGGTAISLI
                     HSLDPDLIILDLGLPDIDGIEVIGRVRACAKTNKIIVVSAREHERDKVEALDGGADDY
                     LTKPFSVTELLARVRVALRNKAQQDNINNDAPKSFEVKNLKIDYENHIVSINGEEIHL
                     TPIEYKIIELMSKYSGRVLTHKFIIDKVWGNYYESENQSLRVFMASIRRKIEKNPAQP
                     EYILTEVGVGYRMADE"
     gene            complement(16225..17832)
                     /locus_tag="ACGI59_001303"
     CDS             complement(16225..17832)
                     /locus_tag="ACGI59_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HFG9739105.1"
                     /translation="MKKERLQHEDLKEYDYDEDGYLSKKIIIKDFIKTILVMTIATGI
                     SVVFEKVGFNESNIILVYILGVLFATSITYGYFFGILSSVIGVLSFNFFFTHPLYTFL
                     AYRKDYPMTFCIMLMAAIITSTLTSRIKREVRLSHKREKMIRLLYENNKTLLKAKNKN
                     QIIEFCGSNLVNILNRTVIVTIADENNNLGKPSIYICNNDGSENIFHSVLEKEAIKEA
                     FRTGNPVGIGTKYCKNSSAYYFPIKGQESNVLGVIGVACFKIDIITENEKILLEAVST
                     QVALAIDRENLFEKNKKANLEAERERLRGNLLRSISHDLRTPLTSILGSSSTILENDD
                     VIDKETRVELLKNIYEDTSWLTHSVENILSMTRIDEGKLDIEKRPEVVDEIIAESILR
                     VKKFANSRDIKTNIPDEIIIVHVDVLLIEQVLVNLIDNAIRHTPKNSKIELTVKKESN
                     QVLFEVADNGKGIPNEDIGNIFNRFYTKNKSRNLERRGIGLGLEICKSIVEAHGGEIV
                     AFNNPSGGATFRFSIPMYDEEGKDKWK"
     gene            complement(18086..19264)
                     /locus_tag="ACGI59_001304"
     CDS             complement(18086..19264)
                     /locus_tag="ACGI59_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9739106.1"
                     /translation="MEKLSDKRRVKAVSMETFVFIVLLAVGFGYVGSIMGAGMMFKVI
                     MSTAHALLLDTVFLIMAMAVLAGALSALLSEFGVISLVNKIFKGLMRPIWGLPGASIA
                     GVVATYLSDNPAIIPFAKDKTFTQYFKKYQVPALCNLGTAFGMGLIVTTFMIAQGKEY
                     VLPAIIGNVAAIIGSIISVRIMLTFTKKYYNYDPKNDTEKQINDKGAKLEEFREIRDG
                     NVFQRTLDAILEGGKLGVEMGMAIIPGVLVVCTLVMLLTFGPSTDPATGQAVYTGAAY
                     EGIKLLPAIGDKISFIIEPLFGFTSPEAIAFPVTALGAVGAAISLVPEFIKSGAITPN
                     DIAVFTAMGMCWSGYLSTHIGMMDALDARPLAGKAILSHTIGGLCAGICAHFIFMLVG
                     "
     gene            complement(19490..19984)
                     /locus_tag="ACGI59_001305"
     CDS             complement(19490..19984)
                     /locus_tag="ACGI59_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFG9739107.1"
                     /translation="MHLFIEVIAAFFTALSFGVLFNMKGKNLILAGIGGSIAWFSYKF
                     LLNIGVTENLCFFIATVCFAIYCELCARIYMTPATTLSVCCLIILVPGYGIYNTMYSV
                     LTNNYIKAVEYGVSTLSCASSIALGLVFITTVFRKVNLYGVLTKIKENEKYKKSINKI
                     KQQK"
     gene            complement(19985..20746)
                     /locus_tag="ACGI59_001306"
     CDS             complement(19985..20746)
                     /locus_tag="ACGI59_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFG9739108.1"
                     /translation="MDIDRILNFSSNAGKAMLQSGGETYRVEETIARICQSFGIDHVD
                     VFATPTAVMASVFVDGKLHSAIKRISSRRVDLNLVHEVNSLSRAISINNLDIELCEKI
                     LDEISEDNYYSDLITIFFAGIAAATFSILFGGNVEEFIAAFTVGILTKFVIMYLSKSS
                     LNDFFVNAIGAVIIASCSILILKLGFIKTLDHLIAGAIMLLVPGLALTNALRDLLDGQ
                     LISGLAKLAEVFFVGVSIAVGMFLVLGLYFKLGGI"
     gene            21448..24282
                     /locus_tag="ACGI59_001307"
     CDS             21448..24282
                     /locus_tag="ACGI59_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFG9739109.1"
                     /translation="MLRKDKVLLSLHKLCENMTLKDLQSDKTGFTTIEIANHSSLDRS
                     NTSKELNNLYNEKKVLKISGKPILFLHASIIENLIGRTLNSDEFSINNCNELLKSSKN
                     DSEDIFSTLLGHDSSLKLPIEKAKSAILYPPKGLNTLLIGPTGVGKSTFAETMYKYAV
                     ESKIFSSESKFVVFNCAEYAENANLLLSNLFGYVKGSFTGANKDKFGIVAQADGGILF
                     LDEIHRLPPEGQEMLFLLMDKGIYRRLGESDIMHKANVFIVCATTEDIHSSLLGTFLR
                     RIPVTINLPALQDRSLKERLSLIKYFFIIESKYTNATIKVHKDVIKSLLLYDCFGNIG
                     QLKSDIQLMSARAFLNYKTGLKDNIEIDLSLVPDYIYIGLLKLNENRSNLNKLSELDY
                     MLFYEFNGNNLEVEYTKELSKYNIDTSNNGFKYSTEIPYLENDIRSMFINYMEKYSTK
                     LLSISSVTHSDYTSNEIFKVINPKIYYAVEHSLKEAEKISRNKYTKQTYIALSMHISA
                     LIENINQDTYNPKYNLYNDKIFLDKDLNIAKIVLSIIENDLQTSLPKDEVKFISMFLN
                     SMYLEPNNQSESIGVIILAHGNSTASSMCDVANSLLGTNHCNAIDMPLDVKVSTILDK
                     TIELVKECDEGKGVLLLTDMGSLIAFGELISEKTKIKTRSIEMVSTPIVLEAVRKSTL
                     PEMTLDELANSLSSLNPYIGRAAIDSTEINYPEKSREYVIITTCITGYGSAMKVADFI
                     NSSLKNISKYNINLVPHNIESFKMYKNKFSNTKILAVVGSVDFNVANTPFIHIEELLN
                     GYGLDMLNKIIEGTFNANSITNIKETNLSYNSLIINFMTQNLELLDSVKLFNYVNSSL
                     FTIIELIDENLKSKFKLGYILHCSFMIERCFKNTTFPYNNITELISKNHELYSLIREA
                     FKPTEAYFNILIPDEEIAYIIDMINEYLNK"
     gene            24325..25569
                     /locus_tag="ACGI59_001308"
     CDS             24325..25569
                     /locus_tag="ACGI59_001308"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFG9739110.1"
                     /translation="MEDEILKGKIKQLTILALIFIFITPVFAFADTPPVPNSSRAALL
                     IDQETKRILFEKNIDEKMPLASLSKMMTFLLAIEAVDKNQVKETDMVKIDKSTASVGG
                     STCKLKDGDEISLGELMQGLMLVSGNDAAIAIAKHIGKTEKNFVNMMNKKAEEIGMID
                     TYYFNPNGLPIYTDPEHKEPPIENMSTAHDIVTLGKYMYDHYENQVTRITTMQVYNDT
                     KKDFTHYNTNPLLVSVPGVDGIKTGYTDNAGYCLAFSMMVPKDAKNERNHRLIGVVLG
                     DGNKKNRISSSATLLKYGKDNFHSKKIAHKGDIIETPCVDGIDDFKITVKVDKDLYGV
                     VSDNENINPKVVFKNMNYPIHKGDIVGVAKYYNDSGKFVGSVDVKSESNIGCIPLKDK
                     IKIKVAKINKKLEIKNSVCFKA"
     gene            complement(25984..27426)
                     /locus_tag="ACGI59_001309"
     CDS             complement(25984..27426)
                     /locus_tag="ACGI59_001309"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9739111.1"
                     /translation="MLNHISKLSGRYYSSKKTGNILSIMESDIFMIENFGAEIIFDII
                     VNVFTAIISLIFLINLQFDLFLVVLFLQLILTFSQNKMTKLISKKISEIRSDSGSLSN
                     LIQEYVSNIMDIVILNSKKNFFNKYLKRERDLIKKDLNLNIIMSSNIAISQILTGLIT
                     ILIYGIGGYKIINNKMTMGELIAFQQYTGMLIGPCIAIVRANTRIAQSKVSINRVFSV
                     IDEKIDIKVDNNGYRIDNSEIKKIDFKNVSFRYGEDLNLVLNDINLQFEKGEVTGIVG
                     GSGCGKSTITNLIYRLWDINEGDILIDGVNIKDINLKSLRNNIDIVTQDQFVFDDTIK
                     NNIVLNNKISDKELDEILEVTCLDDFIKNLDKGLDEVVGEKGVKISGGQKQRISIARA
                     LVSNKKVLILDEATSALDNISQKEILNNLKEYIQDKIVIIIAHRLSTIKSADNIYVLD
                     KGRVIESGNDAELLHKDGLYNKLVETADFA"
     gene            complement(27441..27725)
                     /locus_tag="ACGI59_001310"
     CDS             complement(27441..27725)
                     /locus_tag="ACGI59_001310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9739112.1"
                     /translation="MKKTINNNLFRILNIIVYKNKTKLIVSFIMVFIIAGVNIVIPQL
                     IRCIIDDGIGNKNIFLLINLILIYIFIRILSATFDVILSYLYTSIKIKLL"
     gene            complement(27709..28383)
                     /locus_tag="ACGI59_001311"
     CDS             complement(27709..28383)
                     /locus_tag="ACGI59_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9739113.1"
                     /translation="MKILKVKDLKKIYGKYENEVRALDGIDLDIEPHKFTAIVGASGS
                     GKSTLLHCVAGLDKPTLGNVYLDDVDLYTLNDDKLSEIRRQEFGFIFQSYNLIPVINV
                     YDNIVLPVLLDGKKEDKEYVMRLINKLGIEKQIKKYPNELSGGQQQRVAIARALANRP
                     SVIFADEPTGNLDSKTTNEVMNMLEWCVKEYKQTLVMITHNDNIAKTADRIITIKDGK
                     IDEKNY"
     gene            complement(28420..29409)
                     /locus_tag="ACGI59_001312"
     CDS             complement(28420..29409)
                     /locus_tag="ACGI59_001312"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HFG9739114.1"
                     /translation="MTKVRIAVLDTGLDVNDDDINGNYVYDSNFQLKDLGGKEINDIN
                     GHGTFVSKTILNISKNTQIYPINIFNDSGKTSSLNLLSTLSKLTMSDIQIINISAASI
                     NSMYEKELEKVCNILNSQGRFIICSKHNKYEDKESIPTIFESVIGVVGNENIYKNNQY
                     IYEPENKIQMYANSKDNFIKFKDKVTHFGKNSKACAVATGIVANILKNNPYIKFNELE
                     EILIQKSKSKEDLKIENLNTPSYKSNLIRLDIARKIINVINRNFSKIKIDFEFVSEYS
                     LFNNFTCIGNYNAYNFLTILNKEFNISIDYKEVYLYQLENLDMAVDLIYENLI"
     gene            complement(29457..30701)
                     /locus_tag="ACGI59_001313"
     CDS             complement(29457..30701)
                     /locus_tag="ACGI59_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFG9739115.1"
                     /translation="MNSSNKYFLDCICEKIINNNQVILSNKVNGRWVKIPIECYEVLK
                     YSVDNALQLGNLYDVFQENEDKKYYERVIDKLEHIGLLSDSILSEFRLERINKISFSP
                     TNRCNLSCDYCCVDSKIDNNDYLDTSQVRKVIDNIVKLNPRILTITGGEPLMRNDFLE
                     LLDYIKEKFKGKIILSTNATLIKEKQIDDIINGLYAIEISLDGYDELSCSEVRGKGVF
                     CKVLNVIRLIKLKKFENVGLSMVVGKNNEKDIEKFNKLNEDLGTTPIIRNFMNIGRGN
                     ENYSKYLNDKLDIDYICKDDYVDESSLCSNMCKAGVNQISIDHKGDVYPCPNLEYKDL
                     KIFNILEFNENILERILNREMDIFNIFDKLKPVNIESCKECKVNLFCTTCPSKIYTLK
                     NNKKMFDANCKKMKNILEPILW"
     gene            complement(30743..33289)
                     /locus_tag="ACGI59_001314"
     CDS             complement(30743..33289)
                     /locus_tag="ACGI59_001314"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFG9739116.1"
                     /translation="MINIKLAVSYLKRQKGKTLSLILSIGIAVMLVFSLSVINESQGQ
                     SDINHAYKNSGDYHAFYEDINSDIVKELNGEEDIKEFNDVLKFGEIISKDNGASLNLC
                     SYNKDFLDSTRYKLVKGREPRTSNELVIEQKVLDKMELDVKLNQTIDFQIVNEHINKN
                     NENSIYSKDKQFKIVGILSKSDEYYENLNKYKVRAFTYSNNGKDIVPYELITHMGTLK
                     LNTKDLDSQKVVNLGRKYKLTSNQFYPNTEVSMSMYMKNISKKTTYSIKQSLLPLLCA
                     TLVIYNMFNIIILDMTKQIGLLRAVGASKRNIRQVFFIQSIVVLSVGIVIGLLGGIVF
                     SYLGLMIVYDKSAELTINTNSIIESIIMSIIAVILASVVPIYKAGNLSIIDAIKKTDK
                     LNYIPRLLLLKSHKKVVGIINEIAFKNIFRNKVTSIIIIITISLCGVLFIGRLTSTKF
                     IYGKESDSANITSKSYGNFDIYLGYSPINANYTFSKYDNSLLNEIKAIEDVTDVEPNI
                     YLKGYLRNADLEEDYLDELKRTEINNNNESTVLIRGYNKELLNNIDKYIDKGENVYSY
                     TDRDYKNVLLVNNFYSRIKLSNNTQIIKSPKIGDLIDIKIPVYKDGEYKYINTKVRIA
                     GIMKNSYISEQDGESQSGGIQIIFNEKDLKELTGISDYNKVFVKIKKETDKKVIKEIN
                     NIIEKSGFSDIEGRYVRNHFGDHYNKSSYKLAMICVFGVLLISSINIIFIVRSNIIVR
                     LSEICTFRAIGMSKKNVKRMLIKENMIYGVLSMIVAGIISSFNYYKIVSETNKLNQQV
                     YGINNSIKFEIPIYEILIFGSISVFVCVIAVYFSNKMINKLSIVSGIKEN"
     gene            complement(33497..33658)
                     /locus_tag="ACGI59_001315"
     CDS             complement(33497..33658)
                     /locus_tag="ACGI59_001315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9739117.1"
                     /translation="MIHIKKIEEKIELQDVEVQADPCKWYYDKHGIISQIDCMVDCAG
                     STPKGTVAY"
     gene            complement(33714..34730)
                     /locus_tag="ACGI59_001316"
     CDS             complement(33714..34730)
                     /locus_tag="ACGI59_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFG9739118.1"
                     /translation="MKFTIFVTEDCNLRCTYCYEGNDKKKENMSYETADKTIDFICNK
                     ELESNSDYTTIVLHGGEPFINFDLIKYIHRDLVDRLSDKKILFDITTNGTIINDEIIE
                     FIAKNVKNISISIDGKKNIHDINRIDANGVGTYEKVMENGKRLIDKGIYVRARMTYNS
                     NTVKYLYESVYDIAKQGFKNIAPISDFMDKNWDNSHIKILNEQISKIMNIKKDYPDID
                     ISILDKQFINKKKGDCFGGINSYAIDTRGDVYPCTVCVGDKDILLGNIYDGLDDKKIK
                     NLIKIYISKNEECDGCTRLDYCMSTRCKLINKKVTGKFQSAVPIYCGLERVYVETTKK
                     LCSL"
     gene            complement(34727..>35997)
                     /locus_tag="ACGI59_001317"
     CDS             complement(34727..>35997)
                     /locus_tag="ACGI59_001317"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFG9739119.1"
                     /translation="VEDLIIYRKNQLVIIYNKKFRKYIKLSEEIYKYFLLGQENKIKI
                     YDFVNYFDDESDKKYIINLIDIMNKNQIFKSNNLNFDMLSNGVAIYFCLTNRCNLFCK
                     HCCVESSSNKKDLLSTYDLKNIVDIISVLNTPHIIITGGEPLIREDFFEIIKYIKQKL
                     PDTKLILSTNGTLIDEHNVDFIITNFEKIDISIDGVDENTCSETRGKGVFNKVISSVN
                     LLKDNGFSNIKLSMVFGQKNSHLNKQFKDLNKKLGTEFVERYFIPTGRGLENKESYLI
                     EDTVLPITIPYILDNELKMCKDKKNKKMGCCACNAGNSQIFINYDGDVYPCPSLIKDD
                     YKMGNIFDNEFVKVIKENKTRDLKSLIKLESIYPYNFEKCKDCDVNIFCWHCPAILDS
                     VKDNELELDRWCSMMKHNLNTAVWGDEKVN"
BASE COUNT        12381 a         5687 c         4069 g        13860 t
ORIGIN      
        1 agatttagct ttatacatta ttttttatat ttttcaaatt gtatactcat aaatccagaa
       61 gtattctaat gtgtttataa tacttttttt catattttaa attaaataac aaatcattat
      121 atttacaagt atcatttatc aataaacaat attaaatgaa gaatatgtct gaagattcaa
      181 agtgatactg atactattaa ttactaaaaa agcagtaaat cttttacaga tttactgctt
      241 atagttataa ataataaaac tatctataat aaatgtacta tactcaatat ttcattcaat
      301 atagtaataa caaatctact ttattcctat gtaaatatga atttcttgtt tctttgtatc
      361 aaaagtatta cattgatact cttcaaaatc acttatataa gtcctatcaa aatccatttg
      421 ccaaaactca aaccaaaaat ctcctacttc tttttgacct cctactataa caaactttgc
      481 atacttacct gctggtataa ctttactaat aatactttct ccaacagtat tattagtgtt
      541 taattcttct aaattcttct ctttatcaga attactattt acttcacaac atgcaacaaa
      601 gctatacgga cttgtaaaat caccctggta atctgtatat agacctattg tcttgtcatt
      661 ctttttaccc tttatagcat tatatatccc tttgcccata aattttttcc acaattcacc
      721 tatatcattt accgcttgtc catctttatt tgtagtctcc ttcgatattc ctataactat
      781 tttctcattt atctctacta tttcataatt catactttat acctcacttt gtttttatta
      841 atctatttta ttttgaacta attcaattat aatttataat atacgacacc tttatgtcat
      901 atttttaata taattaaact cagtgagata ttatatacta aatgcaagat attatatatt
      961 taataaaatc tttaatctag cattatatta taatgaataa attttagaca actcccttag
     1021 tctagttatt acattatccc taacatcttt aggttcaagt acttcaacat tgtcaccaaa
     1081 tgataaaata tatccataaa tccattcacc aataggaaat tctgtaatta catcatatgt
     1141 atcatcttca tttttagtta ttaaatcctt attaaaatca tcaaatactc taaaaagcat
     1201 cttttcttta aacctcaatt tcaacgttat agtattttcc ttaattactt tagaatcatt
     1261 caaacatact tcttctatta tctttcttga aaatacttca tcctttatag ataaattcct
     1321 tatcctacta attctaaata tcctaaaatc atcttttagt ttacaaaatc catacaagta
     1381 ccaagtctta ctcttataca taagcttcag tggttcgaca gttctgtttg tctggcttga
     1441 caaactattt acataattaa aatttatagt attacgcctt aaaatagctt cttttatttt
     1501 tgtaaattta tcatcttcat taaaattact tccccattca gtaaagtcta tttcaatcca
     1561 gtttgaaaaa ttctgctctc caaatattga gccaatttta tttactactg aatttatttc
     1621 tggatactta gtcgcctgta atgttttcag tgcaactatt aaactctctt tatctttttt
     1681 tgacaatata gccttgtcta tagaatattc ttctattaaa gatatccctc ctcctttgcc
     1741 tttcgtcata tatactggta ctcccgacat agacagagtt tctatatctc tatatatggt
     1801 tcttgtagaa acttcaaatc tatctgccaa ctctttagct gttactaatt ttttatttaa
     1861 tagaattact attatttctg ttaatctatt tattttcatt cattaccacc tactaaaatt
     1921 attaaattta tttaatctaa attaatcata ctaattttta aataaataaa atcttagaat
     1981 cttatctaaa ttatttttac atgattttat ttactttcta ttatctttct cactttggat
     2041 attatcatct taatttattt attactatat caatttactt attatcatct catttgttat
     2101 cgtcttactt aatttactta tttctatatc aattcactta ttattacctc acttgttatc
     2161 atcttactta atttacttat ttctatatca atttacttat tatcatctca tttgttatcg
     2221 tcttacttaa tttacttatt gctatgtcaa ttcatttatt attatctcac ttgttatcgt
     2281 cttacttaat ttacttattt ctatatcaat tcacttatta tcatcttaat tcatttgctt
     2341 attatcattt actttattca atttctatat caaaattagt atttgtactt aatacttaaa
     2401 attaatttaa agtctttaca tattatttat tttctattgt aatattcatt tccatacttt
     2461 actaagctac ttccactcat ttattttaaa atttcaattt actatttctc aactctatat
     2521 tattattttt caattttata ttactaattt taaagctttt tagctattat tcctacaata
     2581 tttaaacaat tttttcataa taaagctata ttacaatata ttcaaaatat gtaatatttt
     2641 agatattttt cgttgacaat gttaaatttt tgttttatcc ttaattaagg acatgttatt
     2701 gacatttctg aaacttattt gaaatagttt tttcatttta tttaggaaaa cgtattatac
     2761 ttaagaaaac gtattttctc atttcagata agtaaaatat ttattctttt ggggaggaaa
     2821 tcatggaaat taataaaaaa cattttattc aagtattttt aacttgctta atcttggtag
     2881 taatatgtga atttataggg caaagaacta tagctattgg aaaagcctct atagtactgt
     2941 tccctatgct ttatgcagtt ataattggac ttataataac accagattta ttaggtaaaa
     3001 aaattaaagc acttaaaaaa gtggttggag aaaaagaaat aaatattgct ggtgaagttg
     3061 ttggaattgc actagtaatg cttggtataa aatatggtac tacagttgga ccaaacattg
     3121 acaaaataat tcaagctgga ccagcgttta tcgctcaaga atttggtcat atacttgcac
     3181 caatcgttgc attacctctt gcattaatgt ttggaatgaa aagagaagct gttggagcta
     3241 ctgcaagtat aagtcgtgaa ccttctttag gagttatatc agaaaaatat gggataaact
     3301 caccagaagg tagtggtgta cttggtacat atttaatggg aacagttctt ggtactatat
     3361 actttagtgt actaggttca atatctattt atagtggtct tcacccatat gctttgggaa
     3421 tggcctgtgg tgttggtagt ggaagtatga tgacagcagc tgctggttca ttatctacca
     3481 tggtaccacc tgaaatggca gatacaatac ttgcctatgc tgcaacaagc aatatgatgt
     3541 ctagtataac aggaattata ttcttagttt ttgtatcctt accatttact aattttatgt
     3601 ataagatttt agaacctaaa ttttcgagaa ataaaaaaac taaagaaatt aaatctacag
     3661 aaggagagtg ctaataaatg tttgctaatt taaatttaaa gaaaagttca tatcaattat
     3721 tggttatgtc tgttattgga gttataattc ttataggaca aagaattagc gttggtacat
     3781 ctatagttac agctcttcct ggtatggtga tgctaatatt agctgctatg gcagctatga
     3841 ttataaaaga tttatttcct aaatcaatct ttccagcttt tggatttgca actataatcg
     3901 gccttttact tagtatgcca tatagtccaa catctgaagt attcttaact aacactaata
     3961 atattaactt tatggctata actactccac tactagcttt tgcaggaatt tctgttggaa
     4021 acaaaataga agctttaaaa gaaatgtcat ggaaaatagt tataatttca cttattgtat
     4081 ttacaactat attctttgca tgtgcgagta tagctcatat agttttaagt attcaaggta
     4141 aaatatagga ggaatttatt atgaataaag aacaattaaa atctctagta ataaaaacta
     4201 tagatgaaaa tagagaaaaa atattaaaaa taggtcaagg aatatacaaa aatccagaat
     4261 atggatataa agaatttaaa actacagaag ctgtttcaaa cttttttgag caagaattaa
     4321 atttatctgt agaaaaaaat atagcagtta caggatgcaa agcagatgca aattctagca
     4381 agaaaggtcc tcatatatct atacttggag aattggatgg tatatcatgt aaagaacata
     4441 aagatgctaa tgaaataggt gcttctcata cttgtggaca taatattcaa atagctggaa
     4501 tgttaggtgc tgctgttggt ttagtaaaaa gtggcgtttt agaacattta gatggtaaag
     4561 tatcttttat ggctactcct gctgaagagt ttattgagtt ggaatacaga gagcaattaa
     4621 aaaaagaagg aaaaataact tattttggtg gaaaacaaga attaataaaa agaggttatt
     4681 ttgatgatat agacatatct atgatgtttc atgcttctga tttaggagaa gataaggctc
     4741 tagttggtcc tgagagtaat ggatttgttg gaaaaaaggt taaatttata ggtaaagaat
     4801 ctcatgctgg ttctgctcca catgatggaa taaatgcttt aaatgcagca atgcttgcta
     4861 taaacaacgt gaatgcactt agagaaactt ttaaagagtc tgaaagagtt agattccacc
     4921 ctatcatcac aaaaggtgga gatatagtaa atgttgttcc tgctgatgtt catatggaat
     4981 catatgttag agctagaact ataaatggta tgattgatgc taatgaaaga ataaataagg
     5041 ctttgatggc tggtggaatg gcagttggag ctgatgttga gataactgaa attcctggat
     5101 atcttccaat attacgatac aaaagtcttg ataatttatt taaaaacaat cttgaagatt
     5161 taggtctaaa aggaaaagtt attgatggtg gagattttac aggctctttt gattttggag
     5221 atgtgtctca tataatgcct actcttcacc ctatgattgg tggtgtaaaa ggagctcttc
     5281 atacaagaga ttttgaaata gttgatgaag atttagctta cataattcct gctaaagcaa
     5341 tggcacttac agttgtagac cttctttttg ataatgctaa ggatgctaaa gatatattag
     5401 atacctttac tccagttatg actaaagaag aatatctaac attcttagaa aaacacgata
     5461 aaactttata agtctatttt tataataggg tggtatctac tgtaaaagtt ataccactct
     5521 ttgtttttgt aacagtatac ttcttttctt ttagttttta gattaaacta aaatatatta
     5581 tcctccttaa aacttttaga gctacgttga aatatcagta gaactatgct atattctttc
     5641 taattaatat aaatttctat tctaattcaa ggtggtgaaa aaatgtttaa tgcaggaaat
     5701 cgaattataa atacatggtt gtaccctatt caagatggct ttgtcttgat tgatactgga
     5761 tatgaaaatg gttttaaaca tttcaaaaaa cgaatttcta aattcaacat aaaaatcaaa
     5821 gatattcgat acatatttct aactcatgct catgatgacc atgttggttt tctaaatcag
     5881 attctagacg aatctcctaa tacaaagatt gttatgcatg ataaagcttt agaaattcta
     5941 tataaaggtc aaaactcttt tattggtggt tgtaccagcc gtactgcctt tctcttttgc
     6001 caaattatga aactatttgg aaaaggtgag catagttttt ctccacttaa acaagaattt
     6061 gaaaaaaggt gtattcttgt gtctgacaag aatcgtaaag aaattgaaaa agaactaaat
     6121 ggaaaaatta tagatacacc aggacatact gatgattcaa tttcactact aattgatgaa
     6181 ggtgttttat tttgtggaga ttctgcaatg aatggatttc caagtactca caaaattacc
     6241 atatttgcag aaagcaaact agaatttatt caatcttgga aaacaatcat caatacaaaa
     6301 cctaaaatga tttatcctgg acatggaaaa ccatttgaat acagtaaatt agaacaaaat
     6361 ctaatctatg taaataaaat aaatttatat cctctacata attcaaaaga tattcacaga
     6421 tttttgtaga aaatacaaag taaatttatt tgattaatta aaaaatctaa cactttacta
     6481 cgctacttat tttaataact taatcttcca ctagcttttc ataaaatttt gttttaaaat
     6541 taattttaaa gtgcatatgt tatttaaata cattagataa aattcgtcat taatctactt
     6601 aaagcatatt taactaacac cactcaacca gaaaattcag aaaactgcat tgacacagga
     6661 aaaagtttat tttatacttt ttttaggaca tattgtggac acgtcttaaa tcgacaaaca
     6721 gaatttaaat tgtaagatta accacaatac ttatttttta attatatttt taaacattag
     6781 ggaggatatt atgaatgata acgtagttaa gaagaaaaac ttttttgaca ggacattaaa
     6841 cagaatagaa actgttggta ataaactacc agacccagtt acaatatttt tagggttatg
     6901 tgtactttta cttatactat ctagtctagt tggttctatg ggtatatcag tagttcatcc
     6961 tggtactgga gaaactataa ccgctgtaaa tttattgact gtagaacagc ttcaaattct
     7021 tttaggaaac atagtcagca atttccaagg gtttgctcct ttgggtctag ttctagtaac
     7081 tatgataggt gctggtgtat gtgacaaaac aggacttatg acagcaacaa tcaaagcatc
     7141 agtaagcaag attcctgaaa caagagtaac tcttgttgta atgactatag gtatgcttgc
     7201 caatatagct tcagatgcag gtacaatatt atttccacca ttagctgcac ttgtatatct
     7261 tggtgttggt agacatccac ttataggttt attttctgga tatgcagcag tctgtctagg
     7321 atttgccgca aacattatga taagtgtcaa tgatatactt gctgcaagtt ttacagttcc
     7381 agcagctcaa atgattgatt ctggatttca agccaatgca acaatgaact tattttttat
     7441 gatagcatca acttttgttc taatactttt ggcaacatgg gtaacagaaa aaattgtagc
     7501 tcctagattt ggtaaatatg aaggaaatgc agaactagat gttgatggaa gtcttagtga
     7561 tgtagaaaag aaagggctta aaaaagctag tataagtata ttaatatatg tggctattat
     7621 agtagctctt agtgtaatcg gtaaaaaacc attcttagct gacccagaaa caggagattt
     7681 actatcaaac aatgctcctc ttatgaaagg tatggtacct ataatagcac tagcattttt
     7741 catacctggt gttgtatacg gaaaaactat aggtaaaata aagaaggata aggatgtagt
     7801 ctctataatg gctagtgcaa tgagtgatat gggtggatat ataatactag catttgctgc
     7861 ttcacaattt ttgcaattat tcacaaatag taatttaggt ctgatactag ctgtaaaggg
     7921 cggagaattt ttaaaatcag caggaataaa tggaccttta ttgttgatag gatttattat
     7981 attatcatgt tttataaatt tatttatagg tagtgcatct gcaaagtggg ctattcttgc
     8041 acctatattt gtaccaatgt ttatgatggt tggatacaat ccagcactta ctcagatggc
     8101 ttatcgtata ggtgattgta taacaaatcc acttagccca ttatttccgt acttccctat
     8161 tatactagca ttcacaagaa aatacgataa agatgctggt atgggtactg taattgctaa
     8221 tatgatacca tactctatat cattcttaat agtgtggact atactattaa tattgtttat
     8281 ggtatttaat attccactag gacctggaat acttccttca tatagcatgt aatttattta
     8341 aatctttcta aaaggagaaa taatttatgt tagataaaaa tagacttata aataatttta
     8401 tggatatggt tagaattgat agcccatcta atcaagaatt agaaatgtct aagtggttgg
     8461 taaactactt aaaagaaaga aatatagatg ccattataga tgatgcaggt gaaaaatatg
     8521 gtggtaatac aggtaatgtc attgcctata taaaaggtga agaaggaagt agaccacttt
     8581 gtttatgtgc acacatggac caagttcaac cttgtttagg tgtaaaacca atactagatg
     8641 gtaatgtagt tagaagtgat ggaactacta cacttggtgc agatgataaa gctggaatag
     8701 ctgctatact tgaagcttta gaacatgtta ttacagaaaa aataccacat agagatattt
     8761 atctttgttt tacaatctgt gaggaagctg gtatgcatgg tgtcaaaaac tttaatcctg
     8821 acaacttgcc ttgtaaagat atggtgattt tagattctgg tggagctatt ggttcgatag
     8881 catacaaagc tcctgctcaa caatctataa aaatctcttt tcaaggaaag aaagcacatg
     8941 ctggtataga acctgaaaaa gggttaaatg ctatattagt agcctctcat gcaataagca
     9001 acatgcacat aggcagaata gattcattga ctacttcaaa tataggtgaa atagaaggtg
     9061 gtggagctac aaacatagta actgataagg ttacattgac tgctgaaatt aggtctcata
     9121 tacctgaaac tttagagtat gagcttaatc atatggaaaa atgttgtaag gacgctgcat
     9181 ctaaatttaa tactacttat acttttgaac ataatatgtc ttaccctagt tttgaactta
     9241 gtagagatag tcatgttttc aagttaagtg aagaagcaat aagacaagtt ggtgttgttc
     9301 caaatccaat ggtaattggt ggtggaagtg acgcaaatat attagctaat cttggatata
     9361 attgtgctat attaagtcta ggtatgtatg atgtacacac agttaatgaa tatgtaaata
     9421 tagacgaatt atatgatact gccaaaatag tttatcatat gattaaatta taaatatcat
     9481 aatagaactc tttaaattat aaaaataaaa acctatgtat cctcaacttt ataagttata
     9541 tattagagtt gttttacata ggttttagta tttttatata ataatttgtt ttaacatttt
     9601 tcataataag ctttgatatt ttatatagta atttgttcta acatttttca taataagctt
     9661 caatattttg cataataatt tattctagca tttttaataa taggctctag tattttatat
     9721 taataattta ttctagtatt ttacataatt atttgttcta atttttttca taataagctt
     9781 taatacttta tatagtaatt tgttgtagaa ttttatacaa tataatgatt tatacagttt
     9841 tataaaatat cttaaaacag tatttcagta atattttttg gtcttcctcc accaatacta
     9901 gtctcttttg cacatattcg acctaaacct gcatttacta ttttatttaa tatacgtctt
     9961 gcacttcttt cacttacatt tagatactct gctaactctt ttgaatcata aatcttactt
    10021 tttctattct catttatagc aataattttt gcaactgact cacaactcat tccagtcttt
    10081 ttagatattt cgattacctt ttcatcagat agcatcaaag aataatttaa ttcattttcg
    10141 gaagctaaag gtcctcttat gttttcatct tcatctacca aatatatacc aatttctttt
    10201 gaatctatgg agtgtcttaa tgctttatgt gcattatttt cagcctgata cgcagttact
    10261 ccagtaccaa ttcctatact aagagaaaag cccatgtctt ttatctctct ctttagattg
    10321 aatagcttat cataattata ctcattatca gctacccctt tatgaacaaa tacaacataa
    10381 tcatttcttc caagtaaaaa taaggaacct tgaatgcttc ttacatagtt tactacaagc
    10441 ttatccatat ctgatttttt tatcatgtca gaataatagt tatctatctt ctctttataa
    10501 tctattaaac tcaatatttc aacagcaatt tgggaaaatc ttgccttact tagagcatat
    10561 tcactcttta ccttgtcata actttctttg attaattgta tagtagcttg taaacgaaat
    10621 acaggatagc ctctttcttt taattcatta tatactgcac caaaaccagt caacattata
    10681 tccgtcttat tttcttcaaa taattttata tgccattcta caagttcaga ctctcctact
    10741 tccaaagaaa atggattaca aaatacactc gttgctttaa tatcaaattc tttaagtgca
    10801 tcttccacaa catatctatc aactacatct atactgaatt tatctaattt tatatttgca
    10861 ctttttattg cccaaaatgc cttcattata ctcgtcccac ttctcggtac aaatgaatgt
    10921 ggcttactta tctcatactt agattttata aattcataaa ctgcacatcc tgtaaatata
    10981 atagcatcac attcgtcttc acatttagaa ataacttcta ttgtatctac tactttttct
    11041 cttacatata ttttagtctt taaatcactg tctatctgtt ttaaatcatc ctttatctta
    11101 aacccagagt ctgaaggacc tataataccc actatcataa acttcgctcc taatccataa
    11161 atattatact ttttaaaaat tatatcctca ctattttcta cttctattta attattttga
    11221 actactccat cctacctaca tttagaatgg aataattagc ttatttttga taaagtaact
    11281 tctcaaaatc agcacttgga ataggcttac aataataata accttgaatt accacacaat
    11341 ctaaagtcac taaaaattca gcttgttctt ttgtctctac accttctgcc actacatcca
    11401 tatctaaatc ttttgccatg gctatagtgt gctttaaaac tattttttct ctgtctgtga
    11461 ttccttcctc attaaagaaa ccacaatcta tctttaatgt atcaatatta atatttttta
    11521 acatatttaa tgaagaatat gctgaaccaa agtcatccat atttaaaaca ataccatcca
    11581 ttctaaggtc ggctattatc ttagtaaata attttatatt tttaataaat aaactttctg
    11641 taagctctaa ttctaaaaaa gattttggta tactatattt atttattaaa cttaaaacaa
    11701 tttctttaaa atgtggatta tgcaaatgta atcttgatac atttacagag ataggaactg
    11761 gctcttcacc taaatctatc cattttctaa tcaaagaaca tacttgttcc catacataca
    11821 tatctaatct gataataaac ccattcttct caaatagtga aataaattta attggaggta
    11881 ttataccttt ttctggatga acccatctaa ccaatgcttc tgcaccaaca attttatttg
    11941 cattaagatt atacttaggt tgtaaatata catggaattg cccattttca agtgcgtatt
    12001 ccatttccct ttccatctgt ttttcttcaa gtgcatcatt tcttatagta tcatcataaa
    12061 atatatagtt attgatactt tgtttcttta cttttttgtg agcatatcct gccatttcac
    12121 acgcatttgt aattggaata tgattatcca aaattctata aaccccaaaa catggtacta
    12181 acatatattc aaattttttt ctatttaagc tatttttaat cttataaatt accttatgaa
    12241 tatctctatc agagtaactc tcataaaaaa tgcaaaatac atctgcatac atacgacaac
    12301 atataccatt agaaccaatg tttttttgaa tcaactctgc aacatatatt aaagctttat
    12361 ctcctcctct aactccataa atgtcattaa ttaccttgaa cttatctata tccatcatta
    12421 taattgcata acgcttgtca ggatatcttt taagcattaa acttatttgt tcacaaaaac
    12481 tttcataatt gtatagtcct gttaatgtat ctttttggtt tttatgtagt tgcataagat
    12541 aatctatatt ttttatagca actaaaatat gtgtttgttt ctcacctaac ttatttttac
    12601 tactaatctt taacatataa attaatgccc aatagtactt tttatctatt cctaaacgtc
    12661 tatactcaaa atttagttct aaatcgtctt gcttgctatt attaagacta tcaagagatt
    12721 ttagaaggtg gttatcatta aataatatta tatcatcagg atgaattaaa tgttttttta
    12781 tgtatgaaaa agttttttca agattttcct ttataggaaa actaaaaata ttgctctctg
    12841 attgataaat tggagtaatt aaattcgtat caatgcaaat agataaaatt atatcatatg
    12901 tattataagc taatttttca taagccttag aatacccttt tatcattaaa tcatcaaaat
    12961 aaaccttatc aactaactct ttatgtttaa ataaaatatt attcgtcgaa tcagctaagt
    13021 cactaattac tttcatttca ttatttctaa gtttgttctt attataatac ctcttcttat
    13081 cttggtacat tctctcatca gcaactttaa ttagcttatg tatatcaata ttatcgtcac
    13141 tccaaatact gcccactgct acactatata tcatctcatc cttaaattct tcacttaacc
    13201 aactcactaa ttcttcaaat agttgttttg atatatcttc acataataca acaaactcat
    13261 ctcctccaat acggtaaaca ttataagaat gaaagtattt ttttattatt acacatgctg
    13321 acacaatagc agaatcgcca ttagcatgtc caaagttatc attaatatct tttaacccat
    13381 ttatatctac aaatacaact cctatggatt taacttttcc tttttgaaaa ttatctatat
    13441 acttaatata tttatttcta ttatatgcac cagtcaagaa gtcatagtag ctcatataat
    13501 ttaatttatt ttctactttt ctttttttca tctcagttaa taaaaaataa gatagggatg
    13561 tcaaaagaga ggtatctcca acagctttat gtggattatc aactccaata aaccctacta
    13621 ctttatcatc ttgttttagt gaagatgtca ttaaactttc aattttttga ggttttaaaa
    13681 tttcatattc tattggctgt tcttctttta tattttctat atcatcaatt attacaaaac
    13741 ctttttgttc gaataataaa aaccaattct ttaattcatt aatatcaaga tttgttagtg
    13801 cctgtttttg ttcatcaaca ccttcattgc accattcata tgtattagtt actttttttc
    13861 tatcttctga tacttcaaaa acatatgctc tatcagcttt atgaaattta ccaatattgg
    13921 ttaatacaag atttacagca tcagaaaaat tagattctgt tataagatgt ttaatacatc
    13981 caactaataa ctcctttatc tctaattttt ctgctattga cttacttata tcttcctttt
    14041 ctgtaatatc aagagctatt tctaatcgtg catgtttgcc ctcccattta attaacttgt
    14101 cctttaatat aaaatgtcta tttgttcgag tattcgtatt ttcccatatg taatattcat
    14161 cttcttttaa aatagagttt gtacaaaatt cgcagggaga atctttcccc tgtaatgctt
    14221 tataacattt taaatgggat atatcattaa gtcccattgc ctttttccca atttcattaa
    14281 tatataaaag ttcatatgta tctaaagaag aaacatatac aatttcatcc aactcatcta
    14341 ttaaatttat tacatccatt tatatatatc tccttttatt cattttccag attgaatttt
    14401 taaagctttt aaaattttgc tatatatttg gcaatgatat tatatttctt tatgttttaa
    14461 aatagtacct taatgaggtt ctatttttta gtaaatagaa ttattattgt gtcaatcgta
    14521 caatagagtt ttgtaatacc taatttaata tatttataat taattatctc attatgtgtt
    14581 tataattaat tctcatataa atgacaaatc tccacctcaa ttaagtattc caatcatata
    14641 tgaagataat acttcattca tttaaaaata taaaactatt atatattaaa atttcttcaa
    14701 taataaatta tctagattgt tactttgata tattatataa tatcacaaag gctgatataa
    14761 aaagacaaaa agggcaattt ttttatatca aaaaaaaatg ttaaattgat attatccata
    14821 tataaattga tattatccat atagatgcta tatttaacaa ttctacttac ttcccttaca
    14881 cttattagca tatagttatt aagagtttgt tgcaaaacaa cacttagcac actttaaaaa
    14941 gtgtggtcta ataagtaatc aacgaagaag acataataaa gcataaaata tgatatactt
    15001 ttccacgcca tcaaaactga ggattttatt attaatatat gctaaaatat gcttaataaa
    15061 ttaaatttta atgggagaat agttatgaaa atatttagtt tcttgttgtt tgtgatagga
    15121 atatgtttat gtattgctgg atttatagga cattacccag tatttgttgg tacaacattg
    15181 agttttcttg caattgcaat gatagctgta agacaagtca aaactaagaa aaaaatgaaa
    15241 taggcaaatc ctaactttac aagttaatat tacacaagta attagtctaa aattggatac
    15301 tttttctatt acagaaatac atcatccact ttgtattttc ttaaaaaaat aaaatagcta
    15361 tgtaaattca agattagata gattaactct ctatccattt cattgtaaaa tatacatagc
    15421 tatttgtatt cgatttaaaa tacttagatt ttcttacata acaaataatt tctatctgat
    15481 tattactcat attcatttag taaccactca gcaactaatc atttagtatc tattcatccg
    15541 ccatacggta acctacccca acctcagtca aaatatattc tggctgagca ggattctttt
    15601 caattttcct tcttatactt gccataaaaa ctctaagcga ctgattttca ctttcatagt
    15661 aatttcccca aactttatct attataaact tatgggttaa gactcttcct gaatacttag
    15721 acatcaactc aataatctta tattcgattg gagttaaatg tatttcttca ccattaattg
    15781 acacaatatg attttcataa tctatcttta ggtttttaac ttcaaaagac tttggagcat
    15841 cattatttat attatcctgt tgagctttat ttctaagagc cacacgtact ctagccaaaa
    15901 gctctgttac actaaaaggt ttagttaaat aatcgtctgc tcctccatct aaagcttcca
    15961 ccttatccct ctcatgctct ctcgctgaaa caacaattat tttattagtc tttgcacatg
    16021 ccctaactcg tcctattacc tcaatcccat caatatctgg tagtcctaaa tccaaaatta
    16081 tcaaatctgg gtcaagagaa tgtataagtg atatagctgt tcctccatta tctgtctcta
    16141 cacacctata attttgagtt tcaagagata cttttataaa tttacaaatt ggcttatcat
    16201 cttctacaat tagaactaat ggctttattt ccacttgtct ttcccctcct catcatacat
    16261 aggtatgcta aatctaaatg tagctcctcc agaaggatta ttaaaggcga caatttctcc
    16321 accatgagct tctactatag atttacatat ctccaatcca agaccaatac ctcttctttc
    16381 aagatttcta gacttattct tagtataaaa cctattaaaa atattcccga tatcttcatt
    16441 tggtattcct tttccattat ctgcaacttc aaatagtact tgatttgact ctttttttac
    16501 agttagttct atcttagaat ttttaggtgt atgccttata gcattatcaa ttagatttac
    16561 taacacttgt tctattaata atacatccac atgaacgatt attatctcat ctggaatatt
    16621 tgtttttatg tctctactat tggcaaactt tttgactctt aatatagatt cagctattat
    16681 ttcatcaaca acttctggtc gtttttcaat atccaatttt ccttcatcta ttctggtcat
    16741 actaagtata ttctcaacag aatgagttag ccaactcgta tcttcatata tatttttcaa
    16801 taattctact ctagtttctt tatctatcac atcatcattt tcaagtattg ttgaagatga
    16861 acctaatata cttgtaagtg gagttcttaa atcatgagaa attgacctaa gcaagtttcc
    16921 tcttaatctc tctctctctg cctcaagatt tgctttttta tttttctcaa ataaattttc
    16981 tctatcaatt gccaacgcaa cctgtgttga aactgcttct aatagtatct tttcattttc
    17041 tgtaataata tctatcttaa aacacgcaac acctataaca ccaagaacat ttgattcttg
    17101 accttttata gggaaataat atgcactgct gtttttacaa tattttgtcc caattccaac
    17161 tgggttacct gtcctaaaag cttcttttat agcttctttt tctaatactg aatggaatat
    17221 attctcactt ccatcattgt tacaaatata aatacttggc ttacctagat tattattctc
    17281 atctgcaata gtcactatta cagttctatt taatatattt actaaattag acccacaaaa
    17341 ttcaataatt tgatttttat tttttgcttt aagcaacgtt ttgttattct catagagcaa
    17401 tcttatcatt ttttctctct tatgagaaag tctcacttct ctttttatcc tagaagttaa
    17461 agtacttgtt ataattgctg ccattagcat aatacaaaag gtcataggat aatcttttct
    17521 ataagctaaa aaagtatata atggatgcgt aaagaaaaaa ttaaatgata gcactcctat
    17581 tactgaagaa agtattccaa aaaaatatcc ataggttata cttgtagcaa atagtacacc
    17641 taatatgtat actagtataa tattagattc attaaaacct accttttcaa aaactactga
    17701 tattccagta gcaattgtca taaccaatat agtttttatg aagtccttaa taattatctt
    17761 tttactgaga tagccatctt catcataatc atactctttt aagtcttcat gttgtagtcg
    17821 ttcctttttc atttactaca attcctcctt attgttactg tttaaattgg ctctatttta
    17881 tataatacaa ttttatcata ataaattcaa tcaatacatt taattaatgt acttaaatta
    17941 ctaaaaggtg ctgtcttaaa atgaaaataa atctctattt caaaacagca cctatatatt
    18001 taaattacta atttagtttt tacatactta aatcaatagt tttattcaat ttcataaatt
    18061 ataatacttc aattataaaa catttctatc caacaagcat aaatataaag tgggcacata
    18121 tacctgcaca taaaccacct atagtatgcg aaagtatcgc tttaccagcc aatggtcttg
    18181 catctaaagc atccatcata cctatgtgtg tacttaaata tccactccaa cacataccca
    18241 ttgctgtgaa tactgctata tcattaggag ttatagcgcc tgattttata aactctggta
    18301 ctaaagatat tgctgctcca acagctccta atgcagttac agggaatgct attgcctctg
    18361 gtgatgtgaa tccaaataat ggttctatta tgaagctaat cttatcacca atagcaggta
    18421 ataattttat accttcatat gcagctcctg tgtaaacagc ttgacctgta gcagggtctg
    18481 tagatggtcc aaaagttaat aacataacta atgtacaaac tactaaaact cctggtatta
    18541 tagccatacc catttcaaca cctaacttac caccttcaag tatagcatcc aatgttcttt
    18601 ggaatacatt accatctctt atttctctaa actcttcaag ttttgcacct ttatcattta
    18661 tttgcttttc agtatcattt ttagggtcat aattataata ctttttagta aaagtcaaca
    18721 taattctaac actaataata ctacctatta ttgccgcaac attacctatt atagctggta
    18781 gtacatattc tttaccttga gctatcataa aagttgttac aataagtccc ataccaaagg
    18841 ctgttcctaa attacataaa gcaggtactt gatacttctt aaaatattgt gtaaatgttt
    18901 tgtctttagc aaatggtata atagctggat tatcagataa atatgttgca actactcctg
    18961 ctatactagc accaggtaag ccccatatag gtctcataag acccttaaat atcttattaa
    19021 ctaaagatat tactccaaac tctgaaagca atgcactaag tgcacctgca agaactgcca
    19081 tagccattat taaaaatact gtgtctaata aaagcgcatg tgctgtactc attattactt
    19141 taaacatcat tcctgcaccc atgatgcttc ctacatatcc aaatcctacc gccaatagta
    19201 caataaatac gaaggtttcc atacttactg ccttgactct tctcttatcc gacagctttt
    19261 ccatcttgaa atcttccccc ttttaatttt aaattaaggt tcctaacatt ggtacaaatt
    19321 ttttggtaca aactgagttc taaaacttac actcgtaatg caagttgtca gttttttgaa
    19381 ataaaaaaaa attacgttta tagaataaga atatcctctt taatttggat actcttattc
    19441 tattttatgt tattatatta tcatcctttt gtttaaaagt caattttttc tatttttgtt
    19501 gtttaatttt atttatactc tttttatatt tttcattttc ttttatcttt gtaagtactc
    19561 catataagtt gacttttcta aatacagtag ttataaatac caatcctaat gcaattgaac
    19621 ttgcacacga taatgtactc accccatatt ctacagcttt tatataatta ttggtcaaaa
    19681 cagaatacat agtattatat attccataac ctggtactaa tattattaaa cagcaaacac
    19741 ttaatgtagt tgctggagtc atataaattc ttgcacataa ctcacaatat atcgcaaaac
    19801 aaactgtagc tataaaaaaa cataagtttt ctgttactcc tatatttaaa agaaatttat
    19861 atgaaaacca agctatactt ccacctattc ctgctagtat taagtttttt cctttcatat
    19921 taaatagcac tccaaagctt aaggcagtaa aaaaagctgc tattacttct ataaacaaat
    19981 gcacttatat cccccctaac ttgaagtaca atccaagtac caagaacatt cctactgcta
    20041 tagatacacc tacaaaaaat acttctgcta attttgcaag accagaaatc aactgtccat
    20101 ctaacaaatc tcttaatgca ttagtcaaag caagcccagg aactaagagc attattgctc
    20161 ctgctattaa atggtctaac gtctttatga atccgagctt taaaattaaa attgaacaag
    20221 aagctataat aactgctcca attgcattta caaaaaagtc attaagggaa gatttactta
    20281 aatacataat aacaaactta gtcagaatcc caacagtaaa tgcagctata aattcttcca
    20341 catttcctcc aaataatatc gaaaaagttg ctgctgcaat acctgcaaaa aatattgtta
    20401 ttaaatctga gtaatagtta tcttcactta tttcatctaa aattttctca cataattcaa
    20461 tatctaaatt atttatactt attgctcttg ataatgagtt aacttcatga accaagttta
    20521 ggtctactct tcttgagctt attcttttaa ttgctgaatg taatttccca tctacaaata
    20581 cagatgccat tacagctgtt ggagttgcaa atacatctac gtggtctatt ccaaaagatt
    20641 gacatattct agctatcgtt tcttcaactc tataggtttc tcctccactt tgcaacatag
    20701 ctttacctgc atttgaggaa aagtttaaaa ttctatctat atccacaaaa catatctccc
    20761 attcttttat agtattattc atattttaat acaagatatt atttatttaa actattttaa
    20821 ataattcata tccaatattt acattttatg aaaaaacttg ttttttatgc ttgtcaaaat
    20881 caaattttaa gcttgtttac accataaaat ggctgaatta atatttgaca aacactttca
    20941 accatttata taaccttcta aattgtataa ctttttacta caaataaaat tatatctctt
    21001 ttaattcttt ctcttgagta tttagcttat agtaacttca tatttatata tgtacaatat
    21061 aaaacattat ttttatgata caaaatataa tttgtaaatg tatatatgta ttaactgtgt
    21121 atatataaat tattcttgac cttatcagtt ttttcatata tcttcatcta taccaaccat
    21181 atattaataa atgatattac atagacttaa gtatattaaa aaacttaaat ttagttattt
    21241 aacaacataa tactctaatt taatctataa aaatccatac ttaatataat gcaactctta
    21301 ttttaacacc atctattaca caaaacaaca tattaaaaag taaaatacaa aatatccact
    21361 ataaacctta ttttaatact gaatatatat tataatatat ttaatgtagc ttaaatatag
    21421 tttaaacata aagaaaggaa acggtatatg ctaagaaaag ataaagttct attatctcta
    21481 cataaattat gtgaaaatat gactttaaaa gaccttcaat cagataaaac aggattcact
    21541 acaatagaaa ttgctaacca ttcatctctt gatagaagta ataccagtaa agaattaaat
    21601 aacctataca atgaaaaaaa agttctgaaa atatctggta agccaatttt atttctacat
    21661 gctagtatta tagaaaattt aattggccgt actttgaaca gtgatgaatt ttctataaat
    21721 aattgtaatg agctacttaa atcaagtaaa aatgactcag aagatatttt ttcaacatta
    21781 cttggacatg attctagtct taaactacct atagaaaaag ctaaatctgc tatactctat
    21841 cctccaaaag gtcttaatac gttactaata ggacctactg gtgttggaaa aagtacattt
    21901 gctgaaacaa tgtacaaata tgctgtagaa agtaaaatct tttcttctga aagcaaattc
    21961 gtagtattta actgtgctga atatgcagaa aatgctaact tattattaag taatttattt
    22021 ggttatgtca aaggttcttt tactggtgct aataaggata aattcggcat agttgcacag
    22081 gctgatggag gcatattatt cttagatgaa atacacagat tgccaccaga aggtcaagag
    22141 atgctttttc ttttgatgga caaaggcata tacagacgat taggagaatc tgacattatg
    22201 cacaaagcta atgtctttat tgtgtgtgcc acaacagaag atatacattc ttctctgctt
    22261 gggacttttt taagaagaat ccctgttaca atcaacctac ctgctttaca ggataggtct
    22321 ttaaaagaaa gattatctct tattaaatac ttctttatta tagaatcaaa atacaccaat
    22381 gcaactataa aagttcacaa agatgtaatt aaatcattac tcctttatga ttgttttggt
    22441 aatataggtc agcttaaatc tgacatacaa cttatgagtg caagagcctt cttaaattac
    22501 aaaactggac ttaaggataa tatagaaata gatttatccc ttgttcctga ttacatctat
    22561 attggtcttt taaaattgaa tgaaaatcgt agtaatctaa ataaattatc agaacttgat
    22621 tatatgcttt tttatgaatt taatggtaat aacttagaag ttgaatatac aaaagaacta
    22681 tctaaataca atatagatac atcaaacaat ggatttaaat actctacaga gattccttat
    22741 ctggaaaatg atataaggag tatgtttata aattacatgg aaaagtactc tacaaaatta
    22801 ctttcgatat cttcagtaac tcattctgat tatacaagta atgagatttt taaagtaatc
    22861 aatccaaaga tttactatgc tgttgaacat tctttaaaag aggctgaaaa aatatcgaga
    22921 aacaaataca caaaacaaac ttatattgct ctttcaatgc atataagtgc acttatagaa
    22981 aatataaatc aagacacata taatcctaag tacaatttat ataatgataa aattttctta
    23041 gataaagatt taaatatagc taaaatagtt ttaagtataa ttgaaaatga cttacaaaca
    23101 tctttaccta aagatgaagt aaaatttata tccatgtttc taaatagcat gtatcttgaa
    23161 ccaaacaatc aatctgaaag tataggagtc ataatacttg ctcatggaaa ttcaacagca
    23221 agcagtatgt gtgatgtagc aaattcattg cttggtacaa accattgtaa tgcaattgac
    23281 atgcctcttg atgtaaaagt aagtaccata cttgataaaa ctatagagtt ggtaaaagaa
    23341 tgtgacgaag gtaagggtgt acttctttta acagatatgg gctctcttat agcttttgga
    23401 gaacttattt cagaaaaaac aaaaatcaaa actcgctcaa ttgaaatggt atctactcca
    23461 atagttttag aagcagtaag aaaaagtaca ctaccagaaa tgactttgga tgaattagct
    23521 aattccttaa gctctttaaa tccatatatt ggacgagcag caatcgattc aacagaaata
    23581 aactatcctg aaaagtcaag agagtatgta ataataacta cttgtataac aggatatggt
    23641 tcagctatga aagtagcaga ctttataaat tcttcactta aaaatattag taaatacaat
    23701 attaatttag ttcctcataa tatagaaagt tttaaaatgt ataagaataa atttagtaat
    23761 actaaaattc tggcagttgt tggaagtgta gattttaatg tagccaacac cccttttata
    23821 cacatagagg aactactaaa tggatatggc ctagatatgc taaacaaaat aatagaaggt
    23881 acattcaatg ccaatagtat tacaaatata aaagaaacaa atctatcata caacagttta
    23941 ataataaact ttatgaccca aaacttagag cttttggact ctgttaaatt attcaactat
    24001 gtcaattctt ccttatttac aataatagaa ttaatagatg aaaacttaaa gagcaaattt
    24061 aagctaggat atatactaca ttgctctttt atgatagaaa gatgttttaa aaatacaact
    24121 tttccatata ataacattac tgaattaatt agtaaaaatc atgaactata tagtttaata
    24181 cgtgaagcct ttaagccaac agaagcttat tttaatattt tgataccaga cgaagaaatt
    24241 gcttatataa tagacatgat taatgagtat ctaaataaat aaaatttata gcacttattt
    24301 tgtgcatatt aattaatata tgaaatggag gacgagattt tgaaaggaaa aataaaacag
    24361 ttaacaatac ttgcattaat ttttatattt ataactcctg tatttgcatt tgcagatact
    24421 ccaccagtac caaattcttc aagagcagca ctacttatag accaggaaac taaaagaata
    24481 ttatttgaaa aaaacattga tgaaaaaatg ccattagcga gtttaagtaa aatgatgacc
    24541 tttttattgg caatagaagc agttgataaa aatcaagtta aagagactga tatggtgaaa
    24601 atagacaagt ctacagcttc agtaggaggt tctacttgta aacttaaaga tggtgatgaa
    24661 atatctctag gagagcttat gcagggtcta atgctagtat ctggaaatga tgctgctata
    24721 gccatagcta aacatattgg taaaactgaa aagaattttg taaatatgat gaacaaaaaa
    24781 gcagaagaaa taggaatgat tgatacgtat tattttaatc caaatgggct tcctatatat
    24841 actgacccag aacataaaga acctcctatt gaaaatatgt caactgctca cgatatagtt
    24901 acacttggaa agtatatgta cgaccattat gaaaatcaag ttacaagaat aaccactatg
    24961 caagtatata atgatactaa aaaggatttt acacattata acacaaatcc tctgcttgtt
    25021 tcagtacctg gtgttgatgg aataaagaca ggatatacag ataatgctgg atattgcctt
    25081 gcattttcta tgatggtacc taaagatgct aaaaacgaaa gaaatcatag attgataggt
    25141 gttgtacttg gtgatggtaa caagaaaaat agaatttctt cttccgctac tttattgaaa
    25201 tatggtaaag ataatttcca ttctaaaaag atagcacata agggtgatat tatagagact
    25261 ccttgtgttg atggtattga tgattttaag ataacagtta aggttgataa agatttgtat
    25321 ggtgttgttt cagataacga aaatatcaat cctaaggtag tgtttaaaaa tatgaattat
    25381 cctatccata agggagatat tgttggtgtt gcaaagtatt ataatgactc tggtaaattt
    25441 gttggaagtg ttgatgtaaa aagtgaaagt aatataggat gtattccttt gaaggataag
    25501 attaagatta aggttgctaa gataaataaa aaacttgaga ttaaaaattc tgtttgtttt
    25561 aaagcatagt aaagcatatt attaaaaggg tatgttaaat ttataaagac tccactttgt
    25621 ggagtctttt tgttttttac aaaaaattaa aatagtggca acaattgagg atgtgaggga
    25681 gagttgccac tattttgtat agggtattat attatgtttt taagctagtt ttactatctt
    25741 ctatgtttat ataataactc ttgtgtgtga atttaattga taattaatat gaaaattatg
    25801 tgaagttttg atttatttgt aatatattct actactatta taatttttct actggatata
    25861 ctaatcattt gtttaacatt atctgagatt gatttgcaaa cttttatata actaaattag
    25921 ttatataact tatagattcc attaaatacc ctaataatca tatatgtaca tacaaagtca
    25981 tgcttatgca aaatctgcag tttcaactaa cttattatat aaaccatctt tatgtagaag
    26041 ttcagcatca tttccagatt ctattactct acctttatct aatacatata tattatcagc
    26101 actcttaatt gttgaaagtc tatgagctat tataataact atcttatctt gtatgtactc
    26161 tttcaagttg ttcaatatct ctttttgtga aatattatct agagctgaag ttgcttcgtc
    26221 cagaataagt acctttttat tacttactaa agctctagct attgatatac gttgtttttg
    26281 acctcctgat attttgacac ctttttctcc tactacttcg tccaatccct tatctagatt
    26341 ttttataaaa tcatctaaac aagtaacttc taatatctca tctaattctt tatcacttat
    26401 tttattattt aatactatat tattttttat tgtgtcatca aatacaaatt gatcttgagt
    26461 tactatatca atattatttc ttagtgattt taaattaata tcctttatat taacaccatc
    26521 tattaatata tcaccttcat ttatatccca aagtcggtat attagatttg ttattgttga
    26581 tttgccacat ccacttccac caacaattcc tgttacttcc cctttctcaa attgtaaatt
    26641 tatatcattt aaaacaaggt ttaaatcctc accatatcta aaagatacat ttttaaaatc
    26701 tattttctta atttcactgt tatctattct atatccatta ttatcaactt taatatctat
    26761 cttttcatct attactgaaa atactctatt gatagacact tttgattgtg ctattctagt
    26821 attcgccctt actatagcta tacatggtcc aattaacatt cctgtatatt gttgaaatgc
    26881 tattaactcc cccatcgtca tcttattatt tattatttta tatcccccaa ttccatatat
    26941 taatattgtt atcaatcctg ttagtatttg agatattgct atattactac tcataatgat
    27001 gttcaaattc aaatcttttt ttatcaaatc tctctctctc tttaaatact tattaaagaa
    27061 attctttttt gaatttaata taacaatatc cataatatta gatacgtact cttgaatcaa
    27121 atttgataaa ctgcctgaat cacttcttat ttcagaaatt ttcttagata taagttttgt
    27181 cattttattt tgagaaaatg ttaatatcaa ctgtaaaaat aaaactacta aaaataaatc
    27241 aaactgcaaa ttaattaaga aaattaacga tattatagct gtaaacacat ttacaataat
    27301 atcaaatata atctctgctc caaagttttc tatcataaat atatcacttt ccataatgga
    27361 taaaatatta cctgttttct tacttgaata atatctacca gaaagttttg atatatggtt
    27421 aagcaacttt acttttagat ttatagtaac tttattttta ttgaagtata taaatatgat
    27481 aatataacat caaatgtagc tgataatatc cttataaaaa tatatatcaa tattaaattt
    27541 ataagcagaa aaatattttt atttcctatt ccatcatcta ttatacatct aattagttga
    27601 ggtattacaa tatttacgcc agcaattata aaaaccatta taaatgatac tatcaactta
    27661 gtcttattct tatatactat tatatttagt attctaaata aattattatt aatagttttt
    27721 ttcatctatt tttccatctt ttatagttat aattctatca gctgtctttg caatattatc
    27781 attatgagtt atcataacta gtgtttgttt gtactcttta acacaccatt caagcatatt
    27841 cataacttca tttgtagtct tactatctaa gttgccagta ggttcatctg caaatattac
    27901 tgacggtcta ttggctaaag ctctagctat tgcaactctt tgttgttgac ctcctgatag
    27961 ttcattcgga tatttcttta tttgtttttc aatccccaac ttattaatta atctcataac
    28021 atattcttta tcttcttttt tcccatcaag caacacagga agtactatat tatcatatac
    28081 atttataact ggtattaaat tgtaactttg aaatatgaag ccaaattcct gtcttcttat
    28141 ttcagacagc ttatcatcat ttaatgtata taaatctaca tcatctaagt acacattccc
    28201 taaagttggc ttgtctaagc ctgcaacaca gtgaagtagt gtacttttac cagaaccact
    28261 tgcacctaca atagctgtaa atttatgtgg ctctatatct aaatcaatac catcaagagc
    28321 tctaacttca ttctcatatt ttccatatat tttttttaaa tcttttacct taagtatttt
    28381 cataacgtat ctccattccc atttaaaaaa catatatttt taaattaaat tttcgtaaat
    28441 aagatctact gccatatcta aattctctaa ttgatataag tatacttctt tgtagtcaat
    28501 actaatatta aactctttat ttaaaatagt taaaaaatta tatgcattat aatttccaat
    28561 acatgtaaaa ttgttaaata aagaatattc acttacaaat tcaaagtcta tttttatttt
    28621 agaaaagttt ctatttatca catttattat tttccttgct atatccaatc ttatcaaatt
    28681 agatttataa cttggagtgt tcaagttttc tatttttaaa tcttctttac ttttgctttt
    28741 ttgaataagt atttcttcca attcattaaa cttaatatat ggattgtttt ttaatatatt
    28801 tgcaacaatg ccagttgcca ctgcacaagc cttactattt ttaccaaaat gagttacttt
    28861 atctttaaat ttaataaaat tatcttttga atttgcatac atttgaatct tattttctgg
    28921 ttcgtatata tattgattat ttttatatat attttcatta cctactacac caataacact
    28981 ttcaaatata gtaggaatac tttctttatc ttcatattta ttatgttttg aacaaataat
    29041 aaatctacct tgactattta aaatattaca aaccttttct aattcttttt catacatact
    29101 attaatagat gcagctgaaa tgtttattat ctgtatatcc gacatagtca acttgctcag
    29161 tgtactaaga agatttaaag aacttgtttt tccagaatca ttaaatatat taataggata
    29221 aatttgggta tttttactta tatttaatat ggttttcgat acaaaagttc catgtccatt
    29281 tatgtcattt atttccttcc cccccaaatc tttcaattga aaatttgaat cataaacata
    29341 atttccatta atatcatcat catttacatc taatcctgtg tctaagactg ctattcttac
    29401 tttagtcaat taataatcac ctctaaaata aattaccttg aatataaaac taatccttac
    29461 cataatatag gttccaagat gtttttcatt tttttacaat ttgcatcaaa catttttttg
    29521 ttatttttta aagtatatat tttcgatgga caagttgtac aaaataaatt tactttacat
    29581 tctttacaac tctcaatatt tactggtttt aatttatcaa atatattaaa tatatccatt
    29641 tctctattaa gaatcctctc taatatgttt tcattaaatt ctaatatatt gaatatcttt
    29701 aaatccttat actctaagtt tggacacgga tatacatctc ctttatgatc tatacttatc
    29761 tgattaacac ctgctttaca catatttgaa cataaactac tttcatccac ataatcatct
    29821 ttgcaaatgt aatcaatatc aagtttatca ttaagatatt tgctatagtt ctcattacct
    29881 ctaccaatat tcataaaatt tcttattata ggcgttgtac ctaaatcttc atttaattta
    29941 ttgaatttct ctatgtcttt ctcattattc tttcctacca ccatagacaa acctacgttc
    30001 tcgaattttt ttagttttat aagtctaatc acatttaata ctttacagaa tactccttta
    30061 cctcttactt cactacaact taattcatca taaccatcta aactaatttc tattgcatat
    30121 aaaccattaa ttatatcatc aatctgtttt tctttaatta aagttgcatt tgtagacaga
    30181 attattttac ctttaaattt ttcttttata taatccaaca gctctaagaa atcatttctc
    30241 atcaatggct ctccacctgt aatggttaat atccttggat ttaatttaac tatattatct
    30301 attacttttc ttacttgaga tgtatctaaa taatcgttat tatctatttt agaatctaca
    30361 cagcaataat cacaacttag attacatcta ttagttggag aaaatgaaat tttgtttatt
    30421 ctctctagtc taaattcact tagaatagaa tcacttagaa gtcctatatg ctctaactta
    30481 tctataactc gttcataata ttttttatct tcattttctt ggaatacatc atacaaatta
    30541 ccaagttgta atgcattatc tactgaatac tttagaactt cataacattc tattggaatt
    30601 ttaacccatc ttccatttac tttattacta agaataactt gattattatt tattattttt
    30661 tcacatatac aatctaaaaa atatttgttt gagctattca tttattttat ttccttccta
    30721 tatatttaaa ttagaaattt cattaatttt cttttatacc acttactata ctaagtttat
    30781 ttatcatttt attagaaaaa taaactgcaa tgacacaaac aaaaacagat atagatccaa
    30841 atattagaat ttcatatatt ggaatttcaa atttaataga gttatttata ccatatacct
    30901 gttgattaag tttgttagtt tcagaaacta ttttataata attgaaacta gatataattc
    30961 cagctacaat catacttaat accccatata tcatattttc ttttattaac attcttttta
    31021 catttttttt ggacatccct attgctctaa atgtacatat ctcactcaac ctcacaatta
    31081 tatttgatct aactataaaa ataatgttta tagatgatat taataaaact ccaaatacac
    31141 aaatcattgc taatttatat gaggatttat tataatggtc tccaaaatga ttacgtacat
    31201 atcttccctc tatatctgaa aatccacttt tttctattat attatttatt tcttttatta
    31261 cttttttatc tgtttctttt tttatcttca caaaaacttt attataatca cttatccctg
    31321 ttaattcttt taaatccttt tcattaaata taatttgaat tccacctgat tgagattctc
    31381 catcttgctc tgatatatag ctatttttca ttattccagc gattcttact tttgtattta
    31441 tatatttata ctctccatct ttatatacag gaatttttat atctattaag tctcctattt
    31501 taggtgattt tataatctga gtattatttg acaatttaat tctagaataa aaattattta
    31561 ccaataaaac atttttataa tccctgtctg tataactata aacattttcg cctttatcta
    31621 tatatttatc tatattattt aatagctctt tattgtaacc tcgaattaaa actgtagact
    31681 cattattatt atttatttca gttcttttta gctcatctaa ataatcttct tctaagtcag
    31741 catttcttag atatcctttt aaataaatat tgggttcaac atctgttaca tcttcaatag
    31801 cttttatttc atttaataaa gaattatcat atttagaaaa tgtataattt gcatttattg
    31861 gtgaatatcc caaataaata tcaaaattgc catatgattt acttgttata ttagctgaat
    31921 ctgactcttt tccatatatg aattttgtag aagttagtct gcctataaat aaaaccccac
    31981 atagtgatat cgtaattatt attattattg atgtaacctt attcctaaat atatttttaa
    32041 aagctatctc atttataatt ccaacaactt ttttatgtga ttttaacaac aaaagcctag
    32101 gaatataatt taacttatct gtctttttta tagcatcaat tatagataaa tttccagctt
    32161 tatatattgg tactacagag gctaatataa ctgctataat tgacatgatg atagattcaa
    32221 ttatagagtt tgtatttata gttaattctg cacttttatc atatactatc atgagaccta
    32281 agtaagaaaa aactatacct ccaagtaaac caattactat acctacagat aaaactacaa
    32341 tactctgaat aaaaaatacc tgtctaatat ttctcttgct agctccaaca gctctaagta
    32401 aaccaatttg cttggtcata tctagtatta ttatgttaaa catattgtat attactaatg
    32461 ttgcacataa taaagggagt aagctttgct ttattgaata agttgttttt tttgaaatat
    32521 ttttcatata catagacata cttacttctg tatttggata aaattgatta cttgttaact
    32581 tatattttct ccctagattc actacttttt gagaatctaa atcttttgta ttcaatttta
    32641 atgtacccat atgagttatt aattcatatg gaacaatatc tttgccattg tttgaatatg
    32701 taaaagctct taccttatat ttatttaaat tttcatagta ttcatcagat ttacttaaaa
    32761 taccaactat tttaaattgc ttatctttac tataaataga attttcatta tttttattta
    32821 tatgctcatt aacaatctga aaatcaatag tttgatttaa tttgacatct aattccattt
    32881 tgtctaaaac tttttgttct attactaatt cattagaagt tctaggctct ctacctttaa
    32941 ctaacttata tctagtagaa tctaaaaaat ctttattgta agagcataaa tttagagatg
    33001 ctccattatc tttacttata atttctccaa atttaagtac atcattgaat tcttttatat
    33061 cttcttcacc attaagttct tttactatat cactatttat atcttcataa aaagcatgat
    33121 aatctcccga gtttttgtat gcatgattta tatctgattg tccttgagat tcattaataa
    33181 cacttaatga aaaaactagc ataacagcaa tccctatact aagtattaat gataaagttt
    33241 ttcctttttg tctctttaaa tatgacaccg caagtttaat atttatcata atcagaatcc
    33301 tccttattca tatttacaag aaaaactcta taacctaaaa tagtaggtta tagagcacta
    33361 ttcattaaat actaactaac tcgttgtgct agttaaattt aattattaaa aatactagag
    33421 ttgagaaaat cacatttcat ttgtaaaaaa ctacatctag tactttcaac atatttaata
    33481 gactgattag tattttttaa taagctactg ttccttttgg agttgatcct gcacaatcca
    33541 ccatacagtc tatttgtgaa attattccat gcttatcata ataccattta catggatctg
    33601 cttgcacctc aacatcttga agttctattt tttcttcgat tttttttata tgtatcataa
    33661 tacatatccc cctttacttt tgttttatat ttattataac tagttaaact agattataaa
    33721 ctacatagtt tttttgttgt ttccacataa actctttcta atccacaata aattggtact
    33781 gcactttgga atttacctgt taccttttta ttgataagtt tacatctggt tgacatacaa
    33841 taatcaagtc tagtacatcc atcacactct tcattcttag atatataaat ttttataagg
    33901 ttcttaattt ttttatcatc taatccatca tatatatttc ctagtaaaat atctttatct
    33961 cctacacata ctgtacatgg atatacatct cctctagtat ctattgcata actatttatc
    34021 cctccaaaac aatctccttt tttcttattt ataaattgtt tatctaaaat agatatatct
    34081 atatcaggat aatccttttt aatattcata attttactaa tttgttcatt taatatttta
    34141 atatgagaat tatcccaatt tttatccata aaatctgaaa ttggtgcgat atttttaaac
    34201 ccttgttttg ctatatcata tacactctca taaagatact taacagtatt tgagttatat
    34261 gtcattcttg ccctaacata tataccttta tctataagtc tttttccatt ttccattact
    34321 ttttcataag tacctactcc gttagcatct attctattta tatcatgtat attctttttt
    34381 ccatcaatac ttatcgatat gttttttaca ttctttgcta taaactctat tatttcatca
    34441 tttataatag ttccatttgt agttatatca aataatatct ttttgtcact caatctatct
    34501 actaaatccc tatgtatata tttaatcaaa tcaaaattta taaatggttc tcctccatgt
    34561 aaaacaattg ttgtataatc actattactt tctaattctt tattacaaat aaaatctatt
    34621 gttttatcag cagtttcata actcatattt tctttttttt tatcattacc ttcataacaa
    34681 tatgtacatc tcaaattgca gtcttcagtt acaaatatag taaatttcaa ttaacttttt
    34741 catctcccca tacagctgta tttaaattgt gtttcatcat actacaccat ctatccaatt
    34801 ccaattcatt atccttaact gaatctaata tcgctgggca atgccaacaa aaaatattta
    34861 catcacaatc tttacatttt tcaaagttat atggataaat actttctaat ttaatcaaag
    34921 actttagatc tcttgtttta ttttctttta taacttttac aaattcattg tcaaatatat
    34981 ttcccatttt atagtcatcc tttatcagtg aaggacaagg atatacatct ccatcataat
    35041 ttataaatat ttgagagttt cctgcattac atgcacaaca tcccattttt ttattttttt
    35101 tatctttaca catttttaat tcattatcta aaatatacgg aattgtaatt ggaagaactg
    35161 tatcttcaat taaatagctt tctttatttt ctaggcctct tcctgttgga ataaaatatc
    35221 tttcaacaaa ttctgttcca agctttttat ttaaatcttt aaattgttta tttaaatgag
    35281 aatttttttg tccaaatacc atagacagct ttatattact gaatccattg tcttttagca
    35341 gattaacaga gcttattact ttattaaata caccttttcc tcgtgtttca ctacaagtat
    35401 tttcatctac tccatcaata ctaatatcta ttttttcaaa attagttata ataaaatcaa
    35461 cattatgttc atctattaaa gttccatttg tggataatat taatttggta tcaggtaatt
    35521 tttgttttat atatttaatt atttcaaaaa aatcttctct tatcaatggt tctccaccag
    35581 ttataattat atgaggagta tttaaaactg atattatatc tacgatattt tttaaatcat
    35641 atgtactaag taaatctttc ttattagaag aagattcaac acaacaatgt ttacaaaata
    35701 aattgcatct atttgtcaaa caaaaataaa ttgcaacacc attgcttaac atatcaaagt
    35761 ttaaattgtt acttttaaaa atttgatttt tgttcataat atctataaga ttaataatat
    35821 attttttgtc actttcatca tcaaaataat ttacaaaatc ataaattttt attttatttt
    35881 cttgtcctaa aagaaaatac ttatatattt cttcagacaa tttaatatat tttctaaatt
    35941 tcttattata aataattact agttgatttt ttctataaat aattaaatct tctacat
//
LOCUS       DAYAPA010000029        35926 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.029, whole genome shotgun sequence.
ACCESSION   DAYAPA010000029 DAYAPA010000000
VERSION     DAYAPA010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 35926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35926
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.029"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(80..754)
                     /locus_tag="ACGI59_001318"
     CDS             complement(80..754)
                     /locus_tag="ACGI59_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFG9739120.1"
                     /translation="MLEIIGMSVEDAKIIEDCGADRIELVSALTEGGLTPSFGLIESV
                     VNSVKIPVNVMIRHHAKSFVYSKEDISIMQKDISVVKEIGANGVVFGVLDKNNSIDEK
                     NLNVLLKCCDNLDVTFHRAIDESNTIDSVKILKDYDKITNILTSGGKGSIVDNIQMIK
                     NMMLSSNHIKILLGGGLNFNNIEKIKELTKASNFHFGTAIRINNSPFEDIDRQKLKQL
                     VNIISR"
     gene            complement(757..1809)
                     /locus_tag="ACGI59_001319"
     CDS             complement(757..1809)
                     /locus_tag="ACGI59_001319"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HFG9739121.1"
                     /translation="MNIISNKIDELKEDLLSDIIDIVKIPSVKGESENGFPFGEKVGE
                     ALNKALEISEKLGFKVRNLDNYIGYAEHGDSDDYVCVIGHVDVVHEGDGWKHQPYKGE
                     ETNGRIYGRGVLDNKGPIMSALYGLYAIKELNLKLDKRVRIIFGTNEESGFEDIPYYL
                     EKEKAPIMGFTPDCKYPVVYGEKGMAKIRIKSKINYEEDVYLGFIENMSENVLVTYKE
                     LNIENSDTILDIKVKYDFSYKLKDVLDEIKASFPNSIDIEVISNFNPVYFDKESNLVK
                     KLQLAYERVTSLDGTPVTTNGGTYAKVMPNIVPFGPSFPGQKGIAHNPDEYMDIEDII
                     LNAKIFANAIYELAKE"
     gene            complement(1858..2817)
                     /locus_tag="ACGI59_001320"
     CDS             complement(1858..2817)
                     /locus_tag="ACGI59_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase"
                     /protein_id="HFG9739122.1"
                     /translation="MWGMIATWRMALEGITEASEHLEKGMEAGDAVELAVRRVEDYPF
                     YKSVGYGGLPNENCEVELDAAFMDGNTLDIGAVAGIRDYSNPVSIARKLSYEKVNNFL
                     VGIGAEDYAHKNGFERKNMLTDRAKLHYKKRKKETLDKGLSPYAGHDTVGMISLDKTG
                     KMCAATSTSGLFMKKRGRIGDSALSGSGFYVDSSIGGATATGLGEDLMKGCISYEIVR
                     LMKEGKHPQEACDIAVNELDKQLIKRRGKAGDLSLVAMNNKGEWGVATNIADFTFSVV
                     TKEESPTVYISNRVDGKTIHKVISNEWMEAYEARIKAPIEVSE"
     gene            complement(2910..3431)
                     /locus_tag="ACGI59_001321"
     CDS             complement(2910..3431)
                     /locus_tag="ACGI59_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrdB-related putative oxidoreductase"
                     /protein_id="HFG9739123.1"
                     /translation="MKKIVMILDQIQAGAGGKEKSNIPPAGKSSPLGPGVMMEQFLNE
                     SKVIATLFCGDEFFVNNQEEVTSKMIAMVKKLNPDVVICGPSFNYENFSKMSAILSKN
                     INDKTDIPAFAAMSEENIDVINEYKNDICIVKTPKKGGIGLNDSLNNICKLAKAIANK
                     EDITLMKEEFCYQ"
     gene            complement(3442..4776)
                     /gene="celB"
                     /locus_tag="ACGI59_001322"
     CDS             complement(3442..4776)
                     /gene="celB"
                     /locus_tag="ACGI59_001322"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0019191 - cellobiose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFG9739124.1"
                     /translation="MNRLERILMPLAEKIGKNKLLIAIRDGFLVSSPLLIIGSLFLLV
                     ANFPIKGWNEFVGQIFGPDWAVKLQQPTVATFEVMTLLAVLGIGYSYAKQIDVDPIAS
                     AAVAIVAFFIITPFVIPYTPEGTESVYMVTGIPLGWMGSKGMFVGMITAITSVKLFAA
                     VVKKGWTLKMPDGVPPTVSKSFAALIPSAIVMVVFFLVKIAFEATPYGSIHEFIFKFL
                     QMPLLKLGNSLGAMAIAYIFLHLFWFFGINGSSVVGAVYNPILKILSAENLAAFQAGS
                     KIPNIITGQFQDMFATFGGAGSTLSLVIAMLLICKSKRIKSMGKLSILPGIFGINEPL
                     IFGLPIMLNPILLIPFAIVPTINIIIAYFCMSAGLVPLTNGVQLPWTTPIIFSGFLTT
                     GWQGAVLQLVLLILGIFMYIPFIKMLDKQYLREENETVEEEDDDISFDDLVL"
     gene            complement(4815..5141)
                     /locus_tag="ACGI59_001323"
     CDS             complement(4815..5141)
                     /locus_tag="ACGI59_001323"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFG9739125.1"
                     /translation="MKRKVYLFCSFGMSTSLLADKMQKVADEHNLPIEVEAFPIAEID
                     KIVEEKNPDCILLGPQVKYMLKELKPKFEAQGKLIDIINEVDYGTMNGEKVLKLAIKL
                     IKSKQA"
     gene            complement(5165..5473)
                     /locus_tag="ACGI59_001324"
     CDS             complement(5165..5473)
                     /locus_tag="ACGI59_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFG9739126.1"
                     /translation="MEEIVLKIIIHAGNAKSMLYEALDYAKENDFKKADELIENANEE
                     ILKAHKVQTELIQKEAGGDKSDISILLIHSQDHLMTCMSERNLIKEMIMLRKEIQKLK
                     "
     gene            complement(5614..7527)
                     /locus_tag="ACGI59_001325"
     CDS             complement(5614..7527)
                     /locus_tag="ACGI59_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFG9739127.1"
                     /translation="MICLNKRQENLLTTLLLKEEFINVNKIANDFECSERTIRNDCKY
                     IDEWLSTFCNAYIKRKPNIGIKFSGDGTDKILVNQKLRGEVKRVNSELHKQVEILNLL
                     LCNNKKVTLNDLSNKLYINKNIVREEISKLSVALNNYNLNISTKKGSGIYIEGDEKDI
                     RIMLVSFLFKYIDKYRLNIDEIEYFHIVDIIIVKNIMLTIEEDMDIRLTDISFKQLML
                     FLLINIIRIRLGNSLKVSNTKKELKLKMLILDIEDELKSNLSIVLNYEEKLFIESLIL
                     GMNKQINSNNIKKSLYLNEELASYTKKIINLVSEESGINFNNDILLYEQLICHLNVTM
                     HQMKSNVYLENPLLDNIKTKFCFLFTVISSSIEIELKGKQITEDEIGYLTLHFQTSLE
                     RKYSKRESNKKASIVCPFSFGVSMLLKVKIEKRFDNIKIIETLREEDLKRDNFNKTID
                     FIIAFQEYENIQKPIFITTPLFTDEDENKLKEFTNKIKEKDTSYKLMNRLMMSDYLIR
                     ELEHDDIYEAITYLTNLLIEKHYAEKEYLQSIITREKSYPTNVGKGILFPHGDMKYIK
                     QSVLCFARLKTPIKIRNGKDIKIILLLAYKKDDDRKVFRELFKEISNLTEDENMLDIL
                     SKCDIEKVKDILI"
     gene            complement(7859..8599)
                     /locus_tag="ACGI59_001326"
     CDS             complement(7859..8599)
                     /locus_tag="ACGI59_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HFG9739128.1"
                     /translation="MINKCLDFIYPENISCIICDKSIKKTNTYSICKSCFKEMNFIQD
                     GCMKCGKPIIRHSIEKEFIEECSYCFNKDFYFDKSISCIEYNDVTKKMVLGLKYNQKT
                     FMAKYIAQIMKEKLYLENIKFDYILFVPLHKKRLNKRGFNQAQKIAFNLSKIVNIPLL
                     DCISRKKYTRMLYKLNKKERKEELKNVFVVKENVKLINNKNILLIDDIFTTGLTTNEI
                     SKLLKLSGANKVFVLTLLTKANDNYIME"
     gene            complement(8644..10863)
                     /locus_tag="ACGI59_001327"
     CDS             complement(8644..10863)
                     /locus_tag="ACGI59_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0043139 - 5'-3' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HFG9739129.1"
                     /translation="MEKLEGMISEIVFKNEDNGYTIAHLVNENDEIVVVGCMPTLAIG
                     ESIEVEGKWVNHKIYGTQFEVNSFMPVTPSSLEGIYVYLSSGMIHGIGEKMAKRIIDK
                     FGVDTLEVIQNSPEKLQEVEGIGSKKVKQIVKSYEEDRELRNIIIQLSPFGITPNYCL
                     KIYKKYKSSAIEVINKNPYQLAEDIRGIGFKVADSIASKIGIDKNSKDRICQGILYTL
                     NKSLSNGHTYLPEHVLIQDSEKLLELNGEIIKECVMMLVYNQKIHIEKVNNENLIYLM
                     PYYLAENGVCSQIVKLSQYEFEDLKIDIDNEINVLEEDKKIKLAEKQILAVKESVNSG
                     VLIITGGPGTGKTTTINAIIDIFENNGKSVTLAAPTGRAAKRMSETSNKEAKTIHRLL
                     EMGFSTDDDLTFFKDEEDPINSDVIIVDEVSMVDIILMYNLLRAIKLGTRVILVGDSD
                     QLPSVGAGNVLKDMINSNIINVVKLNEIFRQAQESMIIVNAHKINNGEPLYLNTKGKD
                     FFFIRKSTNEEILNEIIGLVNERLPKFYKVDKLKDIQVLSSMRKGELGVTNLNIELQK
                     YLNKKEKFKVEENFSKRLFRVGDKVMQVKNNYTKKWETEDQKESGEGIYNGDIGYVYH
                     IDKDKKTIYILFDQTKIVSYLYDELDEIDHSFCTTIHKSQGSEFPVVVLPIAWAPPML
                     LSRNLLYTAVTRAKKLVVLVGDVKYLEYMIKNNRVNQRYSNLGYKLNKFKQEGLLIE"
     gene            complement(11127..12224)
                     /locus_tag="ACGI59_001328"
     CDS             complement(11127..12224)
                     /locus_tag="ACGI59_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9739130.1"
                     /translation="MSGKVNIKTVISFAGAYVATVIGSGFATGQEILQFFTFYGYAGI
                     IGGIISMVLFSWFGAEVIDKGRELKLKEPIKIYQVYCGKYLGTFFEWFGPLFLFGTLV
                     IMIAGAGATLSEYYGLNPYVGRIGMAIVSLITVSLGLTRLSKILGNIGPIIIIFTLLV
                     GAISLFSNIDALSNAGNMVDSLNIKTATSNGYFSGVLYTCYNVIIVITFLTGMGASAV
                     NKKDAVWGGIVGGVALMAAAIMMNLALLSDIGNIYTKEIPALYLADKISPIIGILFSV
                     VLLLGIYTTAVPLLWSVTNRFVEDDHPKFKIITIVVSILACIGGLLPFDKLVGTLYPY
                     TGYMGILILLCMLYRRITKTEGYKENKSEIS"
     gene            complement(12539..13732)
                     /gene="metK"
                     /locus_tag="ACGI59_001329"
     CDS             complement(12539..13732)
                     /gene="metK"
                     /locus_tag="ACGI59_001329"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFG9739131.1"
                     /translation="MARHLFTSESVTEGHPDKICDQISDSILDALLEKDPQSRVACET
                     TVTTGLVLVAGEISTSAYVDIPKLVRETVREIGYTRAKYGFDCDTCAVITSIDEQSGD
                     IAMGVDEGLESKTGEEIEEEIEKVGAGDQGIMFGFACNETPELMPLPISLAHKLSRRL
                     TEVRKTGLVDYLRPDGKTQVTVEYEGSKAVRVHTVLISAQHCETVSNDKIREDLINHV
                     IKEVIPAELLDEETKIYINPTGRFVIGGPQGDTGLTGRKIIIDTYGGYSRHGGGAFSG
                     KDPTKVDRSAAYAARYVAKNIVAAGLADKCEIELAYAIGIARPLSIFIDTFGTGKVSE
                     EKLVELVNKHFDLRPGAIIRDLDLRKPLYKKVAAYGHFGRTDIDLPWERTDKVEQLRK
                     DALGE"
     regulatory      complement(13869..13972)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            14161..14670
                     /gene="yyaC"
                     /locus_tag="ACGI59_001330"
     CDS             14161..14670
                     /gene="yyaC"
                     /locus_tag="ACGI59_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore protease YyaC"
                     /protein_id="HFG9739132.1"
                     /translation="MYLAKVNYSDDDVIQMLSSVLKTIINENTIVVCIGTDRAIGDTL
                     GPLVGTILKNSNFKYPVYGTLDNPIHALNIYESLDTIKNTHIQGNFLAIDACLGSQSN
                     IGNIQIREGPILPGKGVGKKLPQIGNYSIVGIVDKIDENNKLSFNNIRLSFILDLAET
                     IALALLVST"
     gene            complement(14781..15209)
                     /gene="fabZ"
                     /locus_tag="ACGI59_001331"
     CDS             complement(14781..15209)
                     /gene="fabZ"
                     /locus_tag="ACGI59_001331"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFG9739133.1"
                     /translation="MLNIDEIKKLIPHRYPFLLVDKITELEVGKRAVGIKNITVNEPF
                     FQGHFPEYPLMPGVLIVEALAQVCGVAMMSVEENKGKLGVFAGIDKVRIKREVRPGDT
                     LTMEVEMTTLRKNIAKADAKAYVGEELVCKGELMFALVEK"
     gene            complement(15225..16244)
                     /locus_tag="ACGI59_001332"
     CDS             complement(15225..16244)
                     /locus_tag="ACGI59_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HFG9739134.1"
                     /translation="MAGADIGIDLGTANVLVYVSGKGIVLEEPSVVAIDKRTDSVLAV
                     GEEAKKMIGRTPGNIVAIRPLRDGVISDYDVTEKMLKSFIDKIVDKKGFGRFFMPRIM
                     VCVPTGVTEVEKRAVEDATRQAGAREVYIIEEPIAAAIGAGVDISQPNGNMIIDIGGG
                     TVDIAVISLGGAVVSESIKVGGDRFDDAIVKYMKKQHNLLIGERTAEKIKFEIGSAFK
                     REEEKYMKITGRNLITGLPSSITINSTEMLEALRECVEQIVVATHAVLEKTPPELAAD
                     IGDVGIIMTGGGSLLYGLDKIIEQRTGITVTIADEPLSCVAKGTGIALGSIDLLETGG
                     SFKRK"
     gene            complement(16441..16701)
                     /gene="spoIIID"
                     /locus_tag="ACGI59_001333"
     CDS             complement(16441..16701)
                     /gene="spoIIID"
                     /locus_tag="ACGI59_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcriptional regulator SpoIIID"
                     /protein_id="HFG9739135.1"
                     /translation="MRSHIEERAIVVAKYILEKNTTVRQTAKTFGVSKSTIHKDVTER
                     LKEINPSLAKEVKNVLDKNKSERHIRGGLATKLKYEKEHKKM"
     gene            complement(16799..17467)
                     /locus_tag="ACGI59_001334"
     CDS             complement(16799..17467)
                     /locus_tag="ACGI59_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HFG9739136.1"
                     /translation="MKKKLLEKDGFYLSLFVCVCLLAVGGVWFTNNNVDKLASNKGIM
                     ENANKDSEEEIHLIEKDKKDAIPTATDSKQNLEKAKSKEENKSSTTKLNYIGDKVIRG
                     YSEKEPSYSKTLDVWETHKGVDISCTKGKEVKSLLNGIVVDVFDDEEYGQSVKIKSDN
                     NIVVVYSNLDKNVSVKKEQKVTEGQSLGTVGSTSQIESEEGIHVHLEAYSGEKSIDPM
                     SLIK"
     gene            complement(17533..18597)
                     /gene="spoIID"
                     /locus_tag="ACGI59_001335"
     CDS             complement(17533..18597)
                     /gene="spoIID"
                     /locus_tag="ACGI59_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein D"
                     /protein_id="HFG9739137.1"
                     /translation="MKNPLVVLLGFVTCSVLVPSLITLVSYKNVELTEKPESPVSINK
                     TIKKSDIEDKGNKEEKNTVNYETVNKKAPIINVYNHITGKTEKMDMENYLCGVLAGEM
                     SSEFDIEALKAQSVAARTYVVYKQEHGKSSKHKNAVVCTDYKHCQEYKSYDTLKKLNG
                     EEWIKNKYSKIQEAVRGTKGQIITYNDKAILPLYFSTSSGKTENSEEVFSAKYPYLKS
                     VESPYDKYSPKFASTLKISNTDFVKSLRRAYSTIVIDVNNLSKQVSITKRSDAGTVEK
                     IKLGNKELTGKDIRTVFKLNSANFDIKFGEGYIDFVVKGYGHGVGMSQWGAEGMAEEG
                     YKYYDILSHYYTDTKIKDIY"
     gene            complement(18684..19937)
                     /gene="murA"
                     /locus_tag="ACGI59_001336"
     CDS             complement(18684..19937)
                     /gene="murA"
                     /locus_tag="ACGI59_001336"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFG9739138.1"
                     /translation="MAKIIVKKSNPLKGSVKIDGAKNAVLPIIAATLLANGKSTLNGV
                     PNLRDVHVISDLLRHVGAEVEYKENTLTVDASNIKTCEAPYELVRKMRASFLVMGPLL
                     ARFNSTKISMPGGCAIGTRPIDLHLKGFKALGAKIEMDHGFVEAATEKLVGNKLYLDF
                     PSVGATENIMMAASLAEGTTIIENAAEEPEIVDLANFLNEMGADVKGAGTNTIKIKGV
                     KELKGAEHNVIPDRIEAATYMVAAAMTKGDITVENVLMEHLKPVVAKLREAGCEITEM
                     DNSVRVVGPKVLKPIDIKTLPHPGFPTDVQAQFMAMLTVANGTGVVIETVFENRFMHV
                     AEFNRMGANIKIDGRSAVVNGVDELHGAAVNATDLRAGAALILCGLIAEGETQIGEIY
                     HIQRGYVDIDKKITALGGQIEIVED"
     gene            complement(19958..20695)
                     /locus_tag="ACGI59_001337"
     CDS             complement(19958..20695)
                     /locus_tag="ACGI59_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwmB family TATA-box binding protein"
                     /protein_id="HFG9739139.1"
                     /translation="MKKVKLAISFFILMIIGMTISYADTSINTRYNRLSKALEISEAN
                     FKFYNVKINSVINHNLSNKEFHNIFIEIINNLKLDKKGINWNQKGSGDKIQLCATIKG
                     SDKEISIKGINKNNKESYIVVDILDNKVYKHKEVIYSNVENVLNKYSSQVDVSACIVG
                     EYTKRLQNNKYDDILQKILYNMSAKQIDEVKDVNFLSITAYSKVLNDYELEYLGEKIN
                     LNIGIKYNEDDGKTLIYIATPIIKLDY"
     gene            complement(21151..22446)
                     /locus_tag="ACGI59_001338"
     CDS             complement(21151..22446)
                     /locus_tag="ACGI59_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFG9739140.1"
                     /translation="MTNDFKKEKKEDYFVNLKAISTEVLQEKVQDNAWVIVDTRLNDA
                     YNGWKLDGVKRGGHIKGAVDFSANWLSVYSDRKDEVLEQALKTKRIDLDKNIVLYDAN
                     GKDALVVADYLSKKGYKYLYKYDIKQWADDENLPMERYKNYQMIVPAFIIKDILDGKI
                     PETFEDSKNIKMIEASWREESYTKGHIPTSVHVNTDIIEPPPTWMLDNDDNLTKFALD
                     YGLTKDDTVIVSSSTPMASYRLAVILRYIGVKDVRVLNGGTNSWLSAGYELEFISNPK
                     HSCTNFGADIPVNSQLIVTTSELRQKLKEKNKFILVDNRTWDEHIGKVSGYTYYDKKG
                     RIPGALYGHSGSDSVSLEEYRNIDNTMRNKYEILEMWDKENIDVNKQLIFMCGSGWRA
                     AEVLTYANVIGVENTSLYSDGWMGWSLDNSNLIEVGEHK"
     gene            complement(22897..24729)
                     /gene="glmS"
                     /locus_tag="ACGI59_001339"
     CDS             complement(22897..24729)
                     /gene="glmS"
                     /locus_tag="ACGI59_001339"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFG9739141.1"
                     /translation="MCGIVGYLGSRKAAEVIVEGLSKLEYRGYDSAGVAVNSSNEKEL
                     NIRKFKGRLSVLAEDLEKNPIDGNLGIGHTRWATHGEPSDVNSHPHFNQAKTIAVVHN
                     GIIENYMEIKEELISEGVKFESQTDTEVIAHLVDKYYEGNLLDAVYKTISKLRGAYAL
                     GVICKEHGNELVAVRKDSPLVVGVGEGENFIASDIPALLKYTRDVYFLENGEVVHLKD
                     ENVTVYDSNRNLVEKEVFHVTWDVEAASKGGYDYFMSKEIHEQPTGVRETLERRLDDN
                     GNIILDSINISKEDLEKINKVYIVACGTAYNAGLLGKYAIEKFVNIPVITDIASEFRY
                     SDPFVDENSLVILVSQSGETADTLAVLRDSKAKGARILSITNVVGSSIARESDDVFYT
                     WAGPEVAVASTKAYTTQITSLYMIALDFAIKKGTITREFYDSMISKMKEIPSKIQEIL
                     DNEEYIKEVAKTVVSSEHAFYLGRGIDYSLAMEGSLKLKEISYIHAEAFAAGELKHGT
                     IALIEKGTPVIAIATQEKLFEKMVSNMEEVRARGAYVVAIAQSHNKDVEKAADKIIYI
                     PNSDDILSPILAVVPMQLLAYHVSVLRGCDVDKPRNLAKSVTVE"
     gene            complement(24996..26342)
                     /gene="glmM"
                     /locus_tag="ACGI59_001340"
     CDS             complement(24996..26342)
                     /gene="glmM"
                     /locus_tag="ACGI59_001340"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFG9739142.1"
                     /translation="MRKYFGTDGVRGVANTELTCDLAYKLGRAGGFVLAQGDHRVKVV
                     VGKDTRISGDMLEASLIAGLMSVGCDVITVGIIPTPAVAYLTRKYGADCGVVISASHN
                     PVEYNGIKFFNKNGYKLDDEIELKIEEYIDDIDKIDCLPIGENVGRKLHEHCAQRDYV
                     DYLKSIISTDFKGLKVVLDCANGASYKVAPIVFDELGASVISINSSPDGNNINYKCGS
                     THPEQLQRAVLEHNADLGLAYDGDADRLIAVNEKGQIVDGDHIMILSALNLKKNNKLA
                     QDTLVVTVMSNIGLTIAAKENGINLSTTAVGDRYVLEDMVKNGYNLGGEQSGHMIFLD
                     YNTTGDGVLSSLILANIILQEKKPLSEIASIMSQYPQVLVNATIKNENKNKYMEYPEI
                     KTEIERIESILDGNGRVLIRPSGTEPLVRVMLEGKEEGQIKELATNLANLIQEKLS"
     gene            complement(26512..27069)
                     /locus_tag="ACGI59_001341"
     CDS             complement(26512..27069)
                     /locus_tag="ACGI59_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HFG9739143.1"
                     /translation="MSTARVICAGFGGQGVMSMGQLLTYAGMLEGKEVSWLPSYGPEM
                     RGGTANCAVTVSNEPVGSPLITDDATAAILLNIPAFEKFKDDVIPGGKIIVNSSLIKE
                     KVDRTDVDVYYIPANELAAELGNDKVANMIMLGAYLKVSDTVDIESVLEAFKKVFGPR
                     KEKFVPLNREALQKGMDATCSKATN"
     gene            complement(27070..27822)
                     /locus_tag="ACGI59_001342"
     CDS             complement(27070..27822)
                     /locus_tag="ACGI59_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HFG9739144.1"
                     /translation="MAVVFKKTEGLQDTQTHYCPGCTHGIIHRLVGEVLEELGVLGDA
                     VGVVPVGCSVLGYKYFNCDTQEAAHGRAPAAATGIKRVHPENTVFTYQGDGDLASIGT
                     AEIVHAAARGEKITTIFVNNTTYGMTGGQMAPTTLVGQRATTAQSGRNAETQGYPIRV
                     SEMLATLTGAVFVERVAVDTPAHVRQAKKAIKKAFQVQQAGLGFGIVEVLSTCPTNWG
                     LAPNDALQWLRDNMIPYYPLGNFKNVEVEEVK"
     gene            complement(27822..28901)
                     /locus_tag="ACGI59_001343"
     CDS             complement(27822..28901)
                     /locus_tag="ACGI59_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HFG9739145.1"
                     /translation="MAKILMKGNEAFGKAAIEAGCKYFFGYPITPQSELPEYLSRELP
                     KIGGAFVQAESEVSAINMVYGGAGAGARVMTSSSSPGVALKQEGITYAVGAEVPCVVL
                     NVMRGGPGLGSIQPSQADYFMSTRGGGNGDYRTPVFAPATVQEAVDMIMEAFDVADYY
                     RSPVMVVADGMIGQMMEPVEFRAPEKKRELPPKDWATVGTKGKRKPNVINSLYLEPEV
                     LEDHCWHLQEKFDAMEKNEVQYEMYKTEDAEFVFAAYGTTSRVVKSAIDILREEGIKA
                     GLIRPKVLWPFPFEAFNQIPNARNILTVEMSMGQMVEDVKMAVEGKLPVYFHGRPGGM
                     TPTPAEIVEKAKKIIAGELVAGGAR"
     gene            complement(28930..29145)
                     /locus_tag="ACGI59_001344"
     CDS             complement(28930..29145)
                     /locus_tag="ACGI59_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFG9739146.1"
                     /translation="MAKGKVSFNQERCKGCGLCVEACPVKIIQLDSNVINKKGYNPAT
                     VFEMEKCIGCASCATMCPDVVITVERD"
     gene            complement(29188..29865)
                     /locus_tag="ACGI59_001345"
     CDS             complement(29188..29865)
                     /locus_tag="ACGI59_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFG9739147.1"
                     /translation="MITNDKRIRIITGHYGSGKSEFAMNYVVKLRDMVSGKVAIADLD
                     VVNVYFRTREKKELMKSLGIQPIDSSINAPTLDLPAVSAEVMSPMVDHSYNSVIDLGG
                     DNVGARVIGRFSHLLKEGDYDMLFVINANREKTQTSEEVIQYIKEIEKSSKLKVTGLI
                     NNTHLIRFTTIDDVLRGQKVAKEVSEKCNIPIRYVACLENLVEQLPKDLEGEIFPIKL
                     YMREDWM"
     gene            complement(29855..30934)
                     /gene="buk"
                     /locus_tag="ACGI59_001346"
     CDS             complement(29855..30934)
                     /gene="buk"
                     /locus_tag="ACGI59_001346"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HFG9739148.1"
                     /translation="MSKIFKILTINPGSTSTKIAVFDNEDLVFEKTLRHSSEEIGKYE
                     KVSDQFEFRKQVIEEALKEGGVKTSELDAVVGRGGLLKPIKGGTYSVSAAMIEDLKVG
                     VLGEHASNLGGIIAKQIGEEVNVPSYIVDPVVVDELEDVARISGMPEISRASVVHALN
                     QKAIARRYAREINKKYEDINLIVAHMGGGVSVGAHKNGKIVDVANALDGEGPFSPERS
                     GGLPVGALVKMCFSGKYTQDEIKKKIKGNGGLVAYLNTNDAREVEERIEAGDEKAKLA
                     YEAMAYQISKEIGASAAVLKGDVKAILLTGGIAYSKMFTEMIADRVKFIADVKVYPGE
                     DEMIALAQGGLRVLTGEEEAQVYDN"
     gene            complement(30956..31858)
                     /gene="ptb"
                     /locus_tag="ACGI59_001347"
     CDS             complement(30956..31858)
                     /gene="ptb"
                     /locus_tag="ACGI59_001347"
                     /EC_number="2.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050182 - phosphate butyryltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019605 - butyrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate butyryltransferase"
                     /protein_id="HFG9739149.1"
                     /translation="MRSFEEVIKFAKERGPKTISVACCQDKEVLMAVEMARKEKIANA
                     ILVGDIEKTKEIAKSIDMDIENYELIDIKDLAEASLKSVELVSQGKADMVMKGLVDTS
                     IILKAVLNKEVGLRTGNVLSHVAVFDVEGYDRLFFVTDAAMNLAPDTNTKKQIIENAC
                     TVAHSLDISEPKVAAICAKEKVNPKMKDTVEAKELEEMYERGEIKGCMVGGPFAIDNA
                     VSLEAAKHKGINHPVAGRADILLAPDIEGGNILYKALVFFSKSKNAGVIVGAKAPIIL
                     TSRADSEETKLNSIALGVLMAAKA"
     gene            complement(32162..33343)
                     /locus_tag="ACGI59_001348"
     CDS             complement(32162..33343)
                     /locus_tag="ACGI59_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HFG9739150.1"
                     /translation="MINRLKNNTKIKIISFLSAIALWMYVMAVVDPEETKGFEDIPVS
                     ISNMSDLKDKNLVIYPDEKLTADVYIKGSLSKVQSVKKEDIHVYGTIEDPIQGQKEVY
                     LKANIPQGVTLEFKHDVLVVNLEKNIKEKRDIKVEVSGNSKRNINKIELSKEFVDVSG
                     PRTLVKEVKSVRANLDVGNETDDFSKKLQLIPLNSDGTKVEGVTLSSSFVTAKIELLK
                     EKIVPIRVSLNDNKDNDVLKNYKLSKEQVTIKGKKEDIDKISYIKTKPIDLADLANGE
                     EKEVSLEIPEGITLEDSSITIKLNDTKQLSAEFLYNAGDIELRNIPTNMDNPNVISDV
                     DIKVVVESMEDLSKLNKKDISLYIDLSQGPEEDSKYKIKYETTYQFKKINIEPNIVEV
                     E"
     gene            complement(33340..34179)
                     /gene="cdaA"
                     /locus_tag="ACGI59_001349"
     CDS             complement(33340..34179)
                     /gene="cdaA"
                     /locus_tag="ACGI59_001349"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HFG9739151.1"
                     /translation="MLDINSFLNGFFNIILRMSIYDLIDISIVAYIFYKIFMFIKDTR
                     AEQVFKGIIFLLLATQLSNTFKLHTVYWISLKALDYGVIAALIIFQPEFRAGLEHIGR
                     AKFNLFGKNVNTSEETLNRNIEEIVEALYSLSRQKIGALIIMERETRISDIINTGTII
                     DAEISRQLLINIFIPNTPLHDGAVVIRDSKVKAAACFLPLTESKDLSKDLGTRHRAGI
                     GVSEVSDCITLIVSEETGGVSIAKAGKLYRDISRERMMNILRSNLKTNTETRSFFKGG
                     IFK"
     gene            complement(35163..35236)
                     /locus_tag="ACGI59_001350"
     tRNA            complement(35163..35236)
                     /locus_tag="ACGI59_001350"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(35200..35202),aa:Arg,seq:tct)
     gene            complement(35246..35320)
                     /locus_tag="ACGI59_001351"
     tRNA            complement(35246..35320)
                     /locus_tag="ACGI59_001351"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(35286..35288),aa:Gly,seq:gcc)
     gene            complement(35331..35407)
                     /locus_tag="ACGI59_001352"
     tRNA            complement(35331..35407)
                     /locus_tag="ACGI59_001352"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(35371..35373),aa:Asp,seq:gtc)
     gene            complement(35413..35488)
                     /locus_tag="ACGI59_001353"
     tRNA            complement(35413..35488)
                     /locus_tag="ACGI59_001353"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(35453..35455),aa:Val,seq:tac)
     gene            complement(35494..35567)
                     /locus_tag="ACGI59_001354"
     tRNA            complement(35494..35567)
                     /locus_tag="ACGI59_001354"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(35533..35535),aa:Gly,seq:tcc)
     gene            complement(35582..35656)
                     /locus_tag="ACGI59_001355"
     tRNA            complement(35582..35656)
                     /locus_tag="ACGI59_001355"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(35621..35623),aa:Glu,seq:ttc)
     gene            complement(35664..35739)
                     /locus_tag="ACGI59_001356"
     tRNA            complement(35664..35739)
                     /locus_tag="ACGI59_001356"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(35704..35706),aa:Met,seq:cat)
     gene            complement(35755..35840)
                     /locus_tag="ACGI59_001357"
     tRNA            complement(35755..35840)
                     /locus_tag="ACGI59_001357"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(35804..35806),aa:Leu,seq:taa)
     gene            complement(35846..35920)
                     /locus_tag="ACGI59_001358"
     tRNA            complement(35846..35920)
                     /locus_tag="ACGI59_001358"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(35886..35888),aa:Asn,seq:gtt)
BASE COUNT        10804 a         6875 c         3926 g        14321 t
ORIGIN      
        1 aatacctatt aacattcata tacccaaatt ataattaact tactctcttt atttaaacta
       61 agtattttat attcatatac tatcttgaaa ttatatttac gagttgttta agcttttgtc
      121 tatctatatc ttcaaaagga ctgttattta ttctaatcgc tgtaccaaaa tgaaaattac
      181 ttgccttagt taattccttt atcttttcta tattattaaa attaagacct ccacctaaaa
      241 gaatttttat gtgattagaa ctaagcatca tattctttat catttgtatg ttatcaacta
      301 tacttccttt ccctcctgat gttaatatat tcgtaatttt atcataatcc ttcaaaattt
      361 taacactatc tattgtattt gattcatcta ttgctctatg aaatgtaaca tcaagattat
      421 cacaacattt caataaaaca tttagatttt tctcatctat actattgttt ttatccaata
      481 caccaaatac aactccatta gcaccaatct ctttaaccac acttatatct ttttgcatta
      541 tacttatatc ttctttactg tatacaaaac ttttcgcatg atgtcttatc attacgttca
      601 caggtatctt tacactgtta acaacacttt ctataagacc aaaacttggt gtcaatccac
      661 cttcagttaa agcactaact aactctattc tatctgcacc acaatcttct ataattttag
      721 cgtcttctac tgacattcct attatttcta acataactac tcctttgcta attcatatat
      781 tgcatttgca aatatctttg catttaatat tatatcttct atatccatgt attcatcagg
      841 attatgagct attccttttt gtcctggaaa tgaaggtcca aacggaacta tatttggcat
      901 tacttttgca tacgtacctc cattagttgt tacgggagtt ccatctaatg atgtaaccct
      961 ttcataagct agctgaagct ttttaacaag attactctct ttatcaaaat atacaggatt
     1021 aaaattagaa attacctcta tatcgatact atttggaaaa ctagctttta tttcatccaa
     1081 tacatctttc aatttataac taaaatcgta ttttaccttt atatctaata tagtatcact
     1141 attttctata ttcagctctt tatatgtaac aagcacattt tcagacatat tttctataaa
     1201 acctaggtat acatcttctt cataattgat tttacttttt attctaatct ttgccatgcc
     1261 tttttcacca tatacaacag gatatttaca atctggtgta aatcccataa taggtgcttt
     1321 ttctttttct aaataatatg gtatatcttc aaatccactt tcttcatttg taccaaatat
     1381 tattctaact ctcttatcta attttaagtt taattcttta atagcatata atccatataa
     1441 ggcagacatt ataggtcctt tattgtctag tactcctcgt ccataaattc ttccattagt
     1501 ttcttctccc ttataaggct gatgcttcca accatctcct tcatgtacaa catctacatg
     1561 tccaattaca caaacataat catcactatc tccatgctct gcataaccaa tataattatc
     1621 caagtttcta accttaaatc ccaatttttc tgatatttct aatgctttat taagtgcctc
     1681 ccctactttt tcgccaaaag ggaagccatt ttctgactcc cctttgacac taggtatttt
     1741 tacaatatct ataatatcac ttaataaatc ttcttttaac tcatctattt tattacttat
     1801 gatattcata aaaatctcct tattataaaa ctctatttta attatgtaac cactacttta
     1861 ctcacttact tctattggtg cttttattct agcctcatat gcttccatcc actcatttga
     1921 tataacctta tgtatagtct ttccatctac tctatttgaa atatatacag taggagattc
     1981 ttctttagtt actactgaaa aagtaaaatc agctatattt gtagctactc cccattcacc
     2041 tttattattc atggcaacta atgataaatc accagctttt cctcttcttt tgataagctg
     2101 tttatcaagt tcatttactg caatatcaca tgcttcttgt ggatgcttcc cttctttcat
     2161 aagtctaact atctcataag atatacatcc cttcattaaa tcttctccca aaccagtagc
     2221 tgtagctcct cctatactac tatcaacata gaatcctgaa ccagatagtg cagaatctcc
     2281 tattctacct cttttcttca taaacaatcc acttgttgat gtagctgcac acatttttcc
     2341 agttttgtct aaagatatca taccaacagt atcatgacca gcatatgggc ttaatccttt
     2401 gtctaaagtc tctttttttc tctttttata atgtaacttc gctctatcag tcaacatatt
     2461 ttttctttca aatccatttt tatgagcata atcttcagca cctattccaa ctaaaaaatt
     2521 atttaccttc tcataactta acttcctagc tatgctgaca ggattagaat aatctcttat
     2581 accagcaact gcgccaatat ctaatgtgtt accatccata aatgcagcgt caagttctac
     2641 ctcacaattt tcatttggaa gtccaccata tccaaccgat ttatagaatg gatagtcttc
     2701 aactcttctt acagcaagct ctacagcatc acctgcttcc atgccttttt ctaaatgttc
     2761 tgatgcctca gttattcctt ctagggccat tctccatgtc gctatcattc cccacatgtt
     2821 aaaattcccc tccttaaatt atatagattt tactaatgta tgcttattat aaatagtata
     2881 atatttaaac ttgctaataa ttaatactct tattgataac aaaactcttc tttcatcaaa
     2941 gttatatctt ctttatttgc tattgctttt gccaatttac atatgttatt taatgaatca
     3001 ttaagaccaa ttccaccttt ttttggagtc ttaactatac atatgtcatt tttatattcg
     3061 tttatcacat ctatattttc ttctgacata gctgcaaaag ctggtatatc tgttttatca
     3121 tttatatttt tagacagtat tgctgacatc ttagaaaagt tttcgtaatt aaagcttggt
     3181 ccacagatta ctacatcagg gtttaatttt tttaccatag ctatcatctt actcgttact
     3241 tcttcttgat tatttacaaa aaattcatcc ccacaaaata aggtagctat taccttagat
     3301 tcatttaaaa attgctccat cattacacct ggtcctaatg ggcttgattt tccagcagga
     3361 ggtatattac ttttctcttt tcctccagct ccagcttgta tttggtccaa tatcataact
     3421 attttcttca taatcttcct cctataatac taaatcatca aaagatatat catcatcttc
     3481 ctcttcaaca gtttcatttt cttctctcaa gtattgctta tctagcattt ttataaatgg
     3541 tatatacata aatattccta gtataagaag tactaattgt aagactgcac cttgccatcc
     3601 tgttgttaaa aaaccagaga atataattgg agttgtccac ggtaactgaa ctccatttgt
     3661 aagaggcact aatcctgctg acatacagaa gtaagctatt attatattta ttgttggaac
     3721 tattgcaaat ggtatcaata gtattgggtt aagcattatt ggtagaccaa atatcaatgg
     3781 ctcatttata ccaaatattc ctggtaaaat tgatagtttt cccattgact ttatacgttt
     3841 acttttacat attaataaca tagctatgac taatgatagt gtactacctg ctccaccaaa
     3901 tgtcgcaaac atatcttgga attgacctgt tattatgttt ggtatttttg aaccagcttg
     3961 aaaagcagct aagttttcag ctgatagaat ttttaatatt ggattataca cagccccaac
     4021 aacactactt ccatttattc caaagaacca gaataaatgt aagaatatat aagcgattgc
     4081 catagcacct aaactatttc ctaattttaa taatggcatt tgtaaaaact taaatataaa
     4141 ttcatgtata cttccatatg gtgtagcttc aaaagctatc ttaactaaaa agaaaactac
     4201 cataactatt gcacttggta ttaatgctgc aaatgatttt gatacagttg gaggaactcc
     4261 atctggcatt ttcaatgtcc atcctttttt tacaactgca gcaaataatt ttacagaggt
     4321 tattgctgtt atcataccta caaacattcc cttagaaccc atccaaccta gtggtatacc
     4381 tgttaccatg taaactgatt ctgttccttc tggtgtatat ggtattacaa atggtgttat
     4441 tataaaaaag gcaactattg caactgctgc tgatgctatt gggtctacat ctatttgctt
     4501 tgcataagaa tatccaatcc ctaaaactgc aagtagagtc attacttcaa atgttgctac
     4561 agttggttgt tgcaatttta cagcccaatc tggaccaaaa atttgaccta caaattcatt
     4621 ccatcctttt ataggaaaat ttgctactaa taaaaataat gaacctatta ttaataatgg
     4681 tgatgatact aaaaaaccat ctctaattgc aattaataat ttatttttac ctattttttc
     4741 agccaatggc ataagtattc tttccaatct attcatctat ccaccctcat ttcctattat
     4801 tttttactat aattctacgc ttgtttagat tttattagct ttatagcaag ttttaatact
     4861 ttttccccat tcatagttcc ataatcaact tcattgatta tatctataag ttttccttga
     4921 gcttcaaact ttggttttaa ctcctttagc atgtatttaa cttgtggtcc taataatata
     4981 caatctggat ttttttcttc aactattttg tcaatttctg ctatcggaaa tgcctctact
     5041 tctattggta aattgtgttc atctgctacc ttctgcattt tatctgcaag taaacttgtt
     5101 gacataccaa agctacaaaa taaatatact tttcttttca ttttttaaat cccccttaat
     5161 ttttttattt taatttttga atttcttttc ttaacataat catttctttt ataaggtttc
     5221 tctcactcat acaagtcatt aaatggtctt gagagtgaat caataaaata gatatatctg
     5281 atttatctcc cccagcttcc ttttgtataa gttcagtttg tactttatgt gcttttaaaa
     5341 tttcttcatt tgcattttca attaattcat ctgctttttt aaaatcgttt tcctttgcat
     5401 agtcaagtgc ttcataaagc atagattttg catttccagc atgaattata atctttaaaa
     5461 ctatttcttc cattttttcc tcctaagttg cttctctact tttagattat ttatacctta
     5521 caaacatata atatcttata aaaacaaaaa aacatgcaca actttttcca tcacatggaa
     5581 aattttgtgc ttatccatag ttcactatga attttatata agaatatctt ttaccttttc
     5641 aatgtcacat ttacttagaa tatctagcat attttcatcc tctgttaaat tagatatttc
     5701 tttaaataat tctctaaata cttttctatc atcatctttt ttatacgcta acaaaagtat
     5761 aatctttata tcctttccat ttctaatttt tattggtgtt tttaaccttg caaaacataa
     5821 aacagattgt tttatatatt tcatgtctcc atgagggaat aatattcctt ttccaacatt
     5881 ggttggatat gacttttctc ttgttataat agattgtaaa tactcttttt ctgcataatg
     5941 tttttcaatt aataaattag ttaaatatgt tatagcttca taaatatcat catgttctaa
     6001 ctctcttatt aaataatcag acatcattaa tctgttcata agtttatatg aagtgtcttt
     6061 ttcttttatt ttgtttgtga actctttaag tttattctca tcctcatcag taaataatgg
     6121 tgttgttata aaaattggtt tttggatatt ttcatactct tgaaatgcaa ttataaaatc
     6181 tatagtctta ttaaagttat ctctttttaa atcttcctct cttaatgttt caataatttt
     6241 tatgttatca aatctctttt ctatctttac ttttaatagc atacttactc caaaactaaa
     6301 tggacataca atacttgcct ttttatttga ttcccttttg ctgtattttc tttctagaga
     6361 ggtttgaaag tgaagtgtaa gataaccaat ttcatcttcc gttatttgtt ttccttttaa
     6421 ttcaatttct attgaacttg atattactgt aaataaaaaa caaaatttag tttttatgtt
     6481 atctaaaagt ggattttcta agtaaacatt gcttttcatt tgatgcattg taacattcaa
     6541 atgacatatt aattgttcat aaagtaatat atcattatta aaatttattc ctgattcttc
     6601 tgatactaaa tttataattt tttttgtata acttgctaat tcttcattta gatataaaga
     6661 tttcttaata ttattactat ttatttgttt gttcataccc aatattaaag attctataaa
     6721 caatttttct tcataattta atactatgga taaattagat ttcaattcat cttctatatc
     6781 caatataagc atttttagct taagctcttt cttagtattt gatactttta aagaatttcc
     6841 cagccttatt cgtattatat ttataagcaa aaatagcatc aattgcttga atgatatatc
     6901 tgtaagtctt atatccatat cctcttctat agtaagcatt atgtttttta caattattat
     6961 atctactata tggaagtact ctatttcatc tatgtttaat ctgtatttat ctatatattt
     7021 aaataagaat gatactaaca ttattcttat gtctttctca tccccttcaa tatatatacc
     7081 actgcctttt ttagtcgaga tatttaaatt ataattattt aatgctactg ataatttact
     7141 tatttcttct ctgacaatat ttttatttat gtacaactta ttagataaat catttaaagt
     7201 aactttttta ttattacaaa gtagtaaatt tagtatctca acttgcttat gaagctcact
     7261 atttactctt ttaacttcac ctcgcaactt ctgatttacc agtattttgt ctgttccatc
     7321 tccactaaat ttaatcccaa tattaggttt tcttttgata taagcattac aaaaagtaga
     7381 taaccattca tcaatatatt tgcaatcatt tcttattgtc ctttctgaac actcaaaatc
     7441 attggcaatc ttattaacat ttataaactc ttcttttaat agcaatgttg ttaataaatt
     7501 ttcttgtctt ttattcaaac atatcacctc tttatgactc aacctaaatt tattatatat
     7561 aaaaaagagc tatacataaa ggtatgctag ctctctttta atatgatatt tagctgacct
     7621 ggtttttgac cccacactcg cctactggag ggttcgcttg ggttcgccgc actactactc
     7681 tctctcgatt ttattatcgg taggtcttac ttctaagccg caccatgatc attaacccaa
     7741 tttttagtgt taacttacgt ggatcccttt gcctgcgact ggcttggact ttcaaccaca
     7801 gacctaaata tcacttattc attttaatat tttaaactaa aattgtcaat atataagatt
     7861 attccataat ataattatca tttgcttttg taagtagagt caatacaaaa accttattag
     7921 cacctgataa ctttaaaagt ttagaaattt cgtttgttgt tagaccagta gtaaatatat
     7981 catctattaa aagtatattt ttattattta ttaatttaac attttcttta acaacaaata
     8041 cattttttaa ttcttctttt cgttctttct tattcaattt atatagcatt cttgtatatt
     8101 tttttctact tatgcaatct aataatggaa tatttactat tttacttaaa ttaaatgcta
     8161 ttttttgtgc ctgattaaaa cctcttttat ttagcctttt tttatgaagt ggaacaaata
     8221 atatataatc aaattttatg ttttctaaat ataatttttc tttcattatt tgagctatat
     8281 acttagccat aaaggttttt tggttatact tcagccctaa caccatcttt tttgtcacat
     8341 cattgtattc aatacaagat attgatttat caaaataaaa gtctttatta aaacaataac
     8401 tacactcttc aataaattct ttttcaattg aatgacgtat tattggtttt ccacacttca
     8461 tgcaaccatc ttgtataaaa ttcatttctt taaaacaact tttacatatt gaataagtat
     8521 ttgttttctt tatggattta tcacaaatta tgcaacttat attctcagga tatataaaat
     8581 ccaaacattt attaatcata tttttaaaat tcttaataaa attatcattg catattttca
     8641 tatttattct atcaacaacc cttcttgttt aaatttattt agcttatatc ctaaatttga
     8701 atacctttga ttaactctat tattttttat catatattca agatatttca catctccaac
     8761 taatacaact aatttttttg ctcttgttac tgctgtatat aataaattac ggctaagtaa
     8821 cataggagga gcccacgcta ttggcaatac tacaactgga aattcacttc cctggctttt
     8881 atgtattgtt gtacaaaaac tatgatcaat ttcatctaat tcatcatata ggtatgatac
     8941 aattttagtc tggtcaaata atatataaat agttttctta tctttatcta tgtgataaac
     9001 atatcctata tctccattat agataccttc tccactttct ttttggtctt ctgtttccca
     9061 tttttttgta taattatttt ttacttgcat taccttatcg ccaactctaa ataatctttt
     9121 agaaaaattt tcttcaactt taaatttttc ttttttgtta agatacttct gtaactctat
     9181 atttagatta gtaactccca attcaccttt tctcattgaa gataaaactt gtatatcttt
     9241 taatttatca actttataaa atttaggtaa tctttcatta accaatccta ttatttcatt
     9301 taaaatttct tcattagttg attttcttat aaaaaagaaa tctttacctt ttgtatttag
     9361 ataaagtggt tctccattgt ttatcttatg ggcatttaca attatcatac tttcttgcgc
     9421 ttgtctaaat atttcattta atttaactac atttatgata ttagaattaa tcatatcctt
     9481 aagtacattt ccagcaccta cagaaggtaa ttggtcacta tctccaacta aaattactct
     9541 tgttcctaac tttatagctc taagtaaatt gtacattaag ataatgtcta ccattgatac
     9601 ctcatcaaca attattacat ctgaatttat tgggtcttct tcatccttaa aaaaagttaa
     9661 atcatcatct gttgaaaatc ccatttctag caacctatgt atagtttttg cttctttatt
     9721 agaagtttca ctcattctct ttgctgctct tcccgttgga gctgccagag tcacactttt
     9781 accattattt tcaaatatat ctatgatagc attaatggta gttgttttac cagttcctgg
     9841 tccaccagtt ataattaata ctccactatt aacagattct ttgactgcta aaatttgttt
     9901 ttctgctaac ttaattttct tatcttcttc taaaacatta atctcgttat ctatatctat
     9961 ttttaagtct tcaaattcat actgtgataa ttttactatt tgactacata ctccattttc
    10021 agctaaatag tatggcataa ggtatataag attttcattg tttactttct ctatatgtat
    10081 tttttgatta taaactagca tcattacaca ctcttttatt atttctccat ttagctccaa
    10141 taatttttcc gaatcttgta ttagaacatg ctctggtaaa tatgtatgcc cattacttaa
    10201 agatttattt aaagtgtata agattccctg acaaattcta tctttagagt ttttatctat
    10261 accaatttta cttgctatac tatcagctac tttaaatcca attcctctta tatcttcagc
    10321 aagttggtat gggtttttat ttataacttc tattgcactg cttttatatt ttttatatat
    10381 ttttaggcag taatttggtg ttattccaaa aggagatagc tgtattatta tatttctcaa
    10441 ttctctgtct tcttcataac tttttactat ttgttttact tttttactcc ctataccttc
    10501 tacttcttgt agtttttctg gtgaattttg tatcacctct aacgtatcta caccaaactt
    10561 atcaattatt ctttttgcca ttttttcacc aataccatgt atcattccag aagataagta
    10621 aacataaatt ccttctaagg atgatggtgt tactggcata aaactattca cttcaaattg
    10681 agtcccataa atcttatgat ttacccactt tccttcaact tctatgcttt ctccaattgc
    10741 taaagtaggc atacatccta caaccacaat ttcatcattt tcatttacca ggtgagctat
    10801 tgtataacca ttatcttcgt ttttaaagac tatttcactt atcattcctt ctaatttttc
    10861 catttttatc tcctaaaaat actaataatc gctccattta aatgttagta taattttata
    10921 tattaaaact atgataatcg tagaaaagtt ttatattttt atacttaacg taatttccaa
    10981 ctattataca ttttatcaga aacaaaaaaa atctgcatcc ataaaatatg gtaccatgaa
    11041 atacaggtac catatttaaa tagctatttt attttaatta ctctatattt agagtataaa
    11101 ttaaatttca taagtatatt acagtactaa cttatttcag atttattctc cttgtacccc
    11161 tcagttttag taattcttct ataaagcata catagtaaaa ttaaaattcc catgtaacct
    11221 gtatatggat atagtgtacc tactaactta tcaaatggca gtaaacctcc tatacaagct
    11281 aatatactta ccactattgt tataatttta aacttagggt ggtcatcttc tacaaatcgg
    11341 tttgttactg accataacaa tggtacagct gtagtgtata ttcctagtaa aagtactact
    11401 gagaataaaa tacctattat tggagatatc ttatcagcta agtaaagtgc tggtatctct
    11461 tttgtgtata tatttcctat gtctgaaagt aatgccaaat tcatcattat agctgctgcc
    11521 ataagtgcta ctccacctac tataccaccc catacagcat ctttcttatt tactgcagaa
    11581 gctcccattc ctgtcaagaa tgttattact attattacat tgtaacaagt ataaagtact
    11641 cccgagaaat atccatttga tgtagcagtt ttaatattta aactatcaac catatttcca
    11701 gcatttgata aagcatcaat attgctaaat aaactaattg ctcctactaa aagtgtaaat
    11761 attattatta taggtcctat attacctagt attttagaaa gccttgtcaa acctaatgaa
    11821 acagttatta gggaaacgat tgccatacca atcctaccta catatggatt taatccatag
    11881 tactctgata atgtagctcc tgctcctgca atcattatta ctaatgttcc aaataaaaac
    11941 aatggaccaa accattcaaa aaatgttcca agatattttc cacaatatac ttgataaatt
    12001 tttattggct ccttaagttt caattctctt cccttatcta taacttctgc tccaaaccat
    12061 gaaaatagta ccatagagat tattccacct ataatacccg catatccata aaacgtaaaa
    12121 aattgcagta tttcttgacc agttgcaaat ccagaaccta ttacagtagc aacataagct
    12181 ccagcaaaac ttataaccgt tttaatattt actttgcctg acatacacta ctccccccaa
    12241 ttaaaattat ttttaaagtg taagtttatt aagattatat tagactcatt attgtgatat
    12301 ttcaatatat ttttaattat ttaacattag ttaacacatt cttaaccatc ctttaacata
    12361 tatcccataa tatgtataaa tttagtagag tattatataa aatgtattga ccagattaaa
    12421 tactgtttat tcattatatg atgataaatt aacatttatt aaaaatattt aactcataat
    12481 aaaaaagctg ttagaaagta atcgtaaaat tacattctaa cagcttttta gttaaaaatt
    12541 attcacctaa agcatctttt cttaactgct caaccttgtc agttctttcc caaggtaaat
    12601 ctatatcagt tctaccaaag tgaccataag cagcaacctt tttataaaga ggctttctta
    12661 aatctaagtc tcttattatc gctcccggtc ttaaatcaaa gtgtttattt actaattcaa
    12721 caagtttttc ttctgaaacc ttgcctgtcc caaaagtatc tataaatata gataatggtc
    12781 tagctatacc tatagcatat gcaagttcta tctcacattt gtctgcaaga ccagccgcaa
    12841 caatattttt tgcaacatat ctagctgcgt aagcagcaga tctatcaact tttgtagggt
    12901 cttttcctga gaaagctcct ccaccatgtc tagaatatcc accataagta tctattatta
    12961 tttttcttcc tgtaagacct gtatctcctt gtggtcctcc tataacgaat cttcctgttg
    13021 gatttatata aattttagtc tcttcatcaa gtaattctgc tggtataact tccttaataa
    13081 catgattaat taaatcttct cttattttat catttgatac tgtttcacaa tgttgagctg
    13141 atataagaac tgtatgtact cttacagctt tacttccttc atattcaaca gtaacttgag
    13201 tttttccatc tggtcttaaa taatcaacta aacctgtttt tctaacttct gtaagtcttc
    13261 ttgataattt atgtgccaaa gatatcggaa gtggcattaa ttctggagtc tcattacatg
    13321 caaatccaaa cattatacct tggtctccag ctccaacttt ttctatttct tcttctatct
    13381 cttctccagt cttactttct agaccctcat caactcccat agctatatct ccagattgtt
    13441 catcaataga agttataact gcgcaagtat cacaatcaaa tccatatttt gctcttgtat
    13501 atccaatttc tcttactgtt tctcttacta atttaggaat gtctacataa gcagatgtac
    13561 ttatttctcc tgctactaaa actaaaccag tagtaactgt agtttcacaa gctactctag
    13621 attgtgggtc tttttctaat aatgcatcta atattgaatc tgatatttgg tcacatatct
    13681 tatcaggatg tccttctgta actgattctg acgtaaataa atgtcttgcc atttttattc
    13741 ccccttattt attcttaatt tttattatag gttttacctt gtttttaatt ttagaaattt
    13801 actcaaaata aaaaaacctc tcctttataa gaagaggtta ataatcgtaa ttacatacta
    13861 attaactgac ctcatctttc aagacttttg tcttgtagga attagcacct tttcccattt
    13921 gggtggttgc tgggcttcac agggcctatc cctcagcctc tcttgataag gtaatcaaga
    13981 tttaagtttt gttttacttt actagcttac tattttttta aaattttgtc aatattttta
    14041 gtcatatttt ttctattcct ttttttgtaa gacaaataaa attttctatc tatctgaaat
    14101 ttaaagttag gtctttttaa tgctttttat tcatatattt ttttgaaggg ggtgttccta
    14161 ttgtatttag ctaaagtaaa ctacagcgat gatgatgtta tacagatgct aagttcagtt
    14221 ctaaaaacta taattaatga aaatacgatt gttgtttgca taggaacaga cagggctata
    14281 ggtgatactt taggcccttt agttggaaca atacttaaga acagtaactt taaatacccc
    14341 gtttacggta cactagataa ccctattcac gccttaaata tatatgagtc tttagatact
    14401 ataaaaaata cacatataca aggtaacttc ttggctatag atgcttgctt ggggtcgcag
    14461 agtaacatag gtaatataca gattagagaa ggacctatcc tgcccggaaa aggtgttggt
    14521 aaaaaactac ctcaaatagg aaattattct atagtaggaa ttgtagataa aattgatgaa
    14581 aataataaac tttcatttaa taatatacgc cttagcttta tattagattt agctgaaaca
    14641 atagccttgg ctttattagt atccacctaa tctaaatata aactaaatga taaaattctt
    14701 tatataaaaa ggtagatatt ttagagtatc tgattaaata ctccaaatta tctacctttt
    14761 tacttttata tttcaagttt ttatttttct actaaagcaa acattaattc acctttacag
    14821 acaagttctt cacctacata tgccttagca tctgccttag ctatattttt tctaagagtt
    14881 gtcatttcaa cttccatagt taatgtatct cctggtctta cttctctctt gattctaact
    14941 ttatctatac ctgcaaatac acctagtttt cctttgtttt cctctacaga catcattgcc
    15001 actccacata cttgtgctag agcttctact attagtactc ctggcatcaa aggatattct
    15061 ggaaagtgac cttggaaaaa tggttcatta actgttatat tctttatacc aacagctctt
    15121 ttcccaactt ctagctctgt aatcttgtct actaataaaa atggatatct atgtggtatt
    15181 aattttttta tttcatctat atttaacatt ctaaactctc ctaattattt tcttttgaaa
    15241 gacccaccag tttcaagtaa gtctattgaa cctaaagcta ttcctgtacc ttttgcaaca
    15301 caagataatg gttcatctgc tattgtaaca gttattcctg tcctctgttc tattatttta
    15361 tctaatccat ataataatga acctcctcca gtcattatta taccgacatc tcctatatct
    15421 gctgctaact caggaggcgt tttttccaaa acagcatgag tagcaactac tatttgctca
    15481 acacattctc taagtgcttc caacatttct gttgaattta tagttataga actaggaagt
    15541 cctgtaatta aatttcttcc tgttatcttc atatattttt cttcttctct cttaaatgca
    15601 gaacctattt caaacttgat tttctctgct gttctttctc ctataagaag attgtgttgt
    15661 tttttcatat atttaactat agcatcatca aatctatctc caccaacctt tatagattca
    15721 cttacaacag ctcctcctag agatattaca gctatatcta cagtacctcc accaatatct
    15781 ataatcatat taccattagg ctgtgatatg tctacaccag ctcctatggc agctgctatt
    15841 ggttcttcta tgatatatac ttctctagct ccagcttgtc tcgtagcatc ttctactgct
    15901 cttttttcca cttctgtgac acctgttggt acgcatacca taattcttgg cataaaaaat
    15961 cttccaaatc cctttttatc aactatctta tctataaaag attttaacat cttctcagtt
    16021 acatcataat ctgaaattac accatctctt aaaggtctta tagcgactat attacctggt
    16081 gttctcccta tcatcttttt agcttcttct cccacagcta aaacagaatc tgttcttttg
    16141 tctatcgcca caacagatgg ttcttctaaa acgattccct taccactaac ataaacaagc
    16201 acatttgcag tacctaaatc tatgcctata tcagctccag ccattttttc aactccttta
    16261 tttataatat tagattatat tataagccta ttttaatact cctataataa agcatataca
    16321 aaaatctcgc tttaatcaag agaaaataaa tcttttgaga gtattattat tttgctattt
    16381 ataattactt aatatatttc tttttataat ttaattcaat aaaaaaagta ggtttttaac
    16441 ctacattttt ttatgttcct tctcatattt taatttcgtt gctaatcccc ctctaatatg
    16501 tctctctgat ttatttttgt ccagcacatt tttaacttct ttggcaaggg atggatttat
    16561 ttccttcaat ctttctgtaa catccttatg aattgtactt ttacttactc caaatgtctt
    16621 ggcggtttgt cttactgtag tgtttttttc aagtatatat tttgctacca ctatggccct
    16681 ttcctctata tgagatctca aagattaccc ccctcacata atagctttaa tgatatatat
    16741 gtaaaaacac caaaaaatat aacaagctta accaaatatg attaaacttg ttatactatt
    16801 acttaattag actcattggg tctatagatt tttctccact atacgcctct aaatgcacat
    16861 gtataccttc ttcactttct atttgagaag tactacctac agtacctaga gattgtcctt
    16921 ctgttacctt ctgttctttc ttaacactaa catttttatc tagatttgaa tatactacta
    16981 caatgttatt gtcacttttt attttgactg attgtccata ttcttcatca tcaaatacat
    17041 ctactactat tccgtttaat agagatttta cttctttgcc tttagtacaa ctaatatcta
    17101 cacctttatg agtttcccaa acatcaagtg ttttagaata actaggctct ttttctgaat
    17161 atcctcttat tactttatct ccaatgtaat ttaattttgt tgtactcgat ttattttcct
    17221 cttttgactt agctttttct aagttttgtt ttgaatccgt tgcagtaggg atagcatctt
    17281 tcttgtcttt ttcaattaaa tgtatttcct cttcactatc tttattagca ttttccataa
    17341 ttcctttatt agaagccaat ttatctacat tattatttgt aaaccaaact ccacctactg
    17401 ctaataaaca aacacataca aacaaagata aataaaaacc atctttttct aacagctttt
    17461 tcttcatttt gcacctccaa atatatttct acatttggat tattgacgtg tttttggcat
    17521 tttatacaat tattagtata tatcttttat ttttgtatct gtgtagtaat gtgataatat
    17581 atcataatat ttataaccct cttctgccat tccttctgct ccccactggc tcataccaac
    17641 accatgacca tatcctttta ccacgaaatc tatgtatcct tctccaaatt ttatgtcaaa
    17701 gtttgcagag ttcaacttaa aaacagttct aatgtctttt ccagtcaatt ctttatttcc
    17761 taattttatt ttttctacag ttccggcatc acttctttta gttattgaaa cttgtttact
    17821 taaattattc acatctatca caatagtgct atatgccctt ctaagacttt taacaaaatc
    17881 tgtgttactt attttaagtg ttgacgcaaa tttaggggaa tacttatcat aaggactttc
    17941 cacagacttt aaataaggat attttgcaga aaatacttct tcactatttt ctgtttttcc
    18001 tgaagatgta gaaaaataaa gaggaagtat cgccttatca ttataagtta ttatttgacc
    18061 tttagttcct ctaactgctt cttggatttt ggaatattta ttcttaatcc attcttcacc
    18121 atttaatttt ttaagagtat cataactctt atattcttga caatgtttat aatcagtaca
    18181 tactactgca tttttatgtt tactagattt accatgttcc tgtttatata ctacatatgt
    18241 tctagctgct acagattgag cttttaaagc ttctatatca aactctgaag acatctcacc
    18301 agctagtact ccacataaat agttctccat gtccatcttt tcggttttcc ctgttatatg
    18361 attatataca ttgataattg gtgctttttt atttacagtt tcatagttca ctgtattttt
    18421 ttcttcttta ttacctttgt cttcaatatc acttttttta atagttttgt ttatagatac
    18481 tgggctctct ggtttttcag ttaattctac atttttataa gaaactaaag ttataagtga
    18541 tggaactaac acagagcaag tcacaaatcc taacaaaaca accaatgggt tcttcatata
    18601 ctctcctcct tagcttttaa aaccttgtat ttaagtatat gaacaaccct attaatttat
    18661 aactttcttc tataaaccaa ttattaatct tctactattt cgatttgacc acctagtgct
    18721 gttatcttct tatctatatc aacataacct ctttggatat ggtatatttc tcctatttga
    18781 gtttctcctt ctgctataag accgcataaa ataagggctg cccctgctct taaatcagta
    18841 gcgtttactg ctgcaccgtg tagctcatct acaccattaa caaccgcact tcttccatct
    18901 atttttatat tagcacccat tctattaaat tcagcaacat gcataaatct gttttcaaaa
    18961 acagtttcta taactacacc agttccattg gctacagtaa gcatagccat aaattgtgct
    19021 tgaacatctg taggaaatcc tggatgtggt aaagttttta tatctattgg ttttaatact
    19081 tttggaccta caactcttac agaattgtcc atttctgtta tttcacaacc agcttctctt
    19141 aatttagcta caactggttt taaatgctcc attaaaacat tttcaacagt tatatcacct
    19201 tttgtcatag ctgctgcaac catataagtt gctgcttcta ttctgtctgg aattacatta
    19261 tgttctgctc cttttaactc tttaacacct tttattttta tagtattagt acctgcacct
    19321 ttaacatctg ctcccatttc gttcaagaag tttgctaaat ctactatttc tggctcttct
    19381 gctgcattct ctattattgt agtcccttct gctaaagaag ctgccatcat tatgttttca
    19441 gttgctccaa cagatgggaa atctaaatat aatttatttc ctactaactt ttctgttgct
    19501 gcttcaacaa aaccatggtc catttctatc tttgcaccta aagctttaaa tccttttaaa
    19561 tgtaaatcta taggtcttgt tcctatagca catcctcctg gcatagaaat tttcgtacta
    19621 ttaaatctag caagcaatgg tcccataacc aaaaaagaag ctctcatctt tcttacaagc
    19681 tcatatggag cttcacaagt ttttatattg cttgcatcaa cagtaagtgt attttcttta
    19741 tattcaactt ctgctcctac atgtcttaat aaatctgata taacatgaac atctcttaaa
    19801 tttggtactc catttaatgt tgattttccg tttgctaata aagtcgctgc tattattggt
    19861 aaaactgcat tttttgcacc atctatttta acactacctt taagtggatt actctttttt
    19921 actattatct tagccatatt ttttgatctc ctcataatta ataatctaat tttataattg
    19981 gtgtggcaat atatatgagt gtttttccgt catcctcatt atatttaatt cctatattta
    20041 aattaatttt ttcccctaaa tattctaatt cataatcatt taatacttta ctatatgctg
    20101 ttatagataa aaagtttacg tctttaactt catctatttg cttagcactc atattatata
    20161 atattttttg taaaatatca tcatatttgt tgttttgtaa cctttttgtg tattctccaa
    20221 caatacaagc agacacatct acttgagatg aatatttatt taacacattt tctacgttac
    20281 tataaataac ctctttatgt ttatatactt tgttatccaa tatgtcaact actatgtaac
    20341 tttctttgtt atttttattt attcctttta tagaaatttc tttatcgcta ccttttatag
    20401 tagcacataa ttgtatttta tcgccagacc ctttttgatt ccaatttatt ccttttttat
    20461 ccaactttag attgttaatt atttctataa aaatattatg aaattcttta tttgataaat
    20521 tatgatttat aacagaatta attttaacat tataaaattt aaaatttgct tctgatattt
    20581 caagagcttt acttaatcta ttgtatctag tatttatact tgtatctgca taagatattg
    20641 tcattcctat aatcattaat ataaaaaagc ttatagctaa ttttactttt ttcatagtaa
    20701 aaaatccccc ttaaatatgt ttttctgtat aatagaatta ttaacaaatt taagagagat
    20761 atactttctc aatatgtatt tttatacatt tttgtatttt tcatctaata atctctttca
    20821 aactcttcaa tctcaatttt ctctatcctt ctcctggact tgccaatttc ttatatgtat
    20881 caaatacatc ttgcattaat tcttatatat tctcttctgt aatataaatt acttagtcta
    20941 tacttaaatc tataatctat aatctataat ctataatcta taatctattc atattttacc
    21001 aacaaaataa acagataata cttttttatc aatttccata gctccaaatg aacataccta
    21061 agtacacttc ccaatacaca atttacacac tcttcctaat aaatcaaaaa cacctttgtt
    21121 ttaaacaact ctattgcact ttcaaaaact ttatttgtgt tctcccactt caattaaatt
    21181 tgaattatca agactccagc ccatccatcc atcactataa agggaggtat tttccactcc
    21241 tatgacattt gcatatgtta atacttcagc agcacgccat ccacttccac acataaatat
    21301 aagctgttta ttcacatcta tattttcctt gtcccacatt tctaaaatct catatttatt
    21361 tctcattgta ttatcaatat tacgatattc ttctagtgaa acactatcac tacctgaatg
    21421 tccatataaa gctcctggta tacgcccttt tttatcataa tatgtatatc cagatacttt
    21481 tcctatatgt tcatcccaag tacggttatc tactagtata aacttatttt tttcttttag
    21541 tttttgtctt aattcagatg tagtaacaat taattgtgag ttaactggta tatctgctcc
    21601 aaaattagta catgaatgtt ttggattact aataaattct aattcatatc ctgctgataa
    21661 ccatgagttt gttccaccat tcaaaactct tacatctttt actcctatat atctcaatat
    21721 aacagctagt ctataagatg ccataggtgt actacttgaa actattacag tatcatcctt
    21781 tgttaatcca tagtctaaag caaacttagt taagttatca tcattatcaa gcatccatgt
    21841 tggaggaggt tctataatat cagtatttac atgaacactt gtaggtatat gcccttttgt
    21901 atacgattct tctctccaac tcgcttcaat catttttata ttctttgaat cttcaaatgt
    21961 ctcaggaatt tttccatcta atatatcttt tattataaaa gcaggaacta tcatttgata
    22021 atttttatat ctttccattg gaagattttc gtcgtctgcc cattgtttaa tatcatattt
    22081 atatagatat ttatatcctt ttttagataa ataatctgct acaaccaaag catcttttcc
    22141 atttgcatca tacaatacta tatttttatc taaatcaatt cttttagttt tcaatgcttg
    22201 ttctaacact tcatcttttc tatctgagta tacacttaac caattagcag aaaaatctac
    22261 tgctcctttt atatgtccac ctctttttac tccatctaac ttccagccat tataagcatc
    22321 atttaagcga gtatcaacta taacccaagc gttatcttgc actttttctt gtaaaacttc
    22381 agtagatatt gccttaagat taacaaaata atcttctttc ttttcttttt taaaatcatt
    22441 tgtcataatt tatactccca atttaaaaaa atttttattt gaaattattt tactaaaata
    22501 aagtaatttt cttatactct attagcttca ttatattctt ttataaatag ttccttatat
    22561 tcttatttta aactactcac tttatcttat cacaaggtta ctaatacctt gtattttaat
    22621 ttttctcata agctattatt tttttatctg atattgacta tatgtttatt aagtactcac
    22681 tatattttat atgaactata tttatcgttc cctatacctg gtatggtcta tatatttatc
    22741 attttacaga tgatttaata tcaatctttt tataaaattt attatagcaa aaacaaaaca
    22801 aatttaatat ttatctaaaa aactcatcct ataaaataca aataacagga tgagttcttt
    22861 tcaataaata ttatctattt aatttttaat ctgtttttat tcaactgtta ctgatttagc
    22921 tagatttctt ggcttatcta catcgcatcc tcttagtaca gagacatgat aagcaagtaa
    22981 ttgcattggt acaactgcta atattggtga taaaatatca tctgaatttg gtatatagat
    23041 tattttatca gcagctttct caacatcctt attatggctt tgagctattg ctactacata
    23101 agctcctctt gctcttactt cttccatatt agaaaccatt ttttcaaata acttttcttg
    23161 agtagctatg gcaattacag gagttccttt ttctattaaa gctattgttc catgctttag
    23221 ctctcctgct gcaaaagctt ctgcatgtat atatgaaatc tcttttaatt tcaatgaacc
    23281 ttccattgct aagctatagt ctatacctct tcctaaatag aatgcatgtt cagaactaac
    23341 aactgtttta gcaacttctt ttatatattc ttcattgtct agtatttctt gtatttttga
    23401 tggtatttct ttcatcttag aaatcatact atcataaaat tctctagtta ttgttccttt
    23461 tttgatagca aagtctaaag ctatcatgta taaagaagtt atttgagtag tatatgcctt
    23521 agttgatgca actgctactt caggtcctgc ccaagtatag aatacatcat ctgattctct
    23581 tgctatagat gaacctacaa cattagttat tgaaagtatt cttgctcctt tagcctttga
    23641 atctcttaat actgctagag tatctgctgt ctcaccagat tgactaacta atattactaa
    23701 tgaattttca tctacaaatg ggtcgctata tctaaattct gatgctatat cagttattac
    23761 tggtatattt acaaattttt ctatggcata cttaccaagt aatccagcat tatatgctgt
    23821 tccacaagcc actatataaa ctttatttat tttttctaag tcttctttag aaatatttat
    23881 actgtctaga attatatttc cattatcatc aagtcttctt tctaaagttt ctcttactcc
    23941 tgttggttgc tcatgaattt cttttgacat aaagtagtca tatccacctt tagacgcagc
    24001 ttctacatcc caagttacat gaaatacttc tttttcaaca agatttctat tagagtcata
    24061 aactgttaca ttttcgtctt ttagatgaac tacttctcca ttttctaaga aataaacatc
    24121 tctagtatat tttaataatg caggtatatc tgatgctata aagttttcac cttcaccaac
    24181 accaactaca agaggactat cttttcttac tgcaactaat tcatttccat gttctttaca
    24241 tataacacct aatgcataag ctcctcttaa ttttgatata gttttgtata cagcatctaa
    24301 taaattaccc tcatagtatt tatctactaa atgtgctatt acctctgtat ctgtttgaga
    24361 ttcaaactta acaccttcag aaattaattc ttcttttatt tccatatagt tttctattat
    24421 cccattatga acaactgcta ttgttttggc ttgattaaaa tgaggatgtg agtttacatc
    24481 agatggttct ccatgagttg cccatctcgt atgccctata cctaaatttc catcaatagg
    24541 attcttttct aagtcttctg caagtacaga aagtctacct ttaaactttc taatattaag
    24601 ttctttttca ttagaactat taactgctac tcctgctgag tcataacctc tgtactctag
    24661 cttagaaagc ccttctacta taacttctgc tgcctttcta cttcctaaat atccaactat
    24721 tccacacatt atttgtgtcc tcctaaaaat attatttata tattttaaga gttaggtcat
    24781 ataagtttac tttggcttcc ctttgtccat aggattgctc ctattattta aagaagtctt
    24841 tcgatggtaa acacaccgca gagcatccgc cgacaattcg attaactctg acctcgtcaa
    24901 cttagttcct atattactca ataattatgt ctataactaa gttctggcgc ttagaaaggt
    24961 gtcctcagta tttatatctg agaataccct ttaaattacg ataatttttc ttgtataaga
    25021 ttagctaagt ttgttgctaa ttcttttatt tgaccttcct ctttaccctc taacataact
    25081 ctaactaatg gctcagttcc tgatggtctt atcaaaactc taccatttcc gtctaaaata
    25141 gattctattc tttctatctc agtttttatt tctggatatt ccatatattt gttcttattt
    25201 tcatttttta ttgttgcatt tactaatact tgtgggtatt ggctcattat tgatgcaatt
    25261 tctgataaag gtttcttttc ttgcaagatt atatttgcaa gtattagaga actaagcact
    25321 ccatctccag tagtgttgta atctaaaaat atcatatgtc cagattgctc tccaccaaga
    25381 ttatatccat ttttaaccat atcttctaaa acatatctgt ctccaactgc tgtagtagat
    25441 aaatttattc cattttcctt agcagctatt gtcaacccta tattactcat aactgtaact
    25501 accaaagtat cttgtgccaa tttattattt ttctttaaat ttaaggcact caatatcatt
    25561 atatggtctc catcaacaat ttgacccttt tcatttactg caattaatct atcagcatct
    25621 ccatcatatg caagacctaa gtcagcatta tgttccaaaa cagccctttg aagttgttct
    25681 ggatgtgtag agccacattt atagtttata ttatttccat ctggtgaact atttattgaa
    25741 attacacttg ctcctagttc atcaaacact attggtgcca ctttatatga cgcaccatta
    25801 gcacagtcca aaactacttt taatccctta aagtcagtgc ttattattga ttttaggtag
    25861 tctacatagt ctctttgtgc acaatgttca tgtaattttc ttcctacatt ttcaccaatt
    25921 ggaagacaat caattttgtc tatatcgtct atatattctt ctattttcaa ttcaatttca
    25981 tcatctaatt tatatccatt cttattaaag aatttgattc cattatattc aacaggattg
    26041 tgagatgcag atataaccac accacaatca gcaccatatt ttctagttaa atatgccact
    26101 gcaggtgttg gtattattcc aacagtaata acatcacatc caacagacat aagtcctgct
    26161 attaaagatg cttccaacat atcacctgat attctagtat ccttacctac tactactttt
    26221 actctgtggt ctccttgagc taagacaaat ccaccagctc taccaagttt atatgctaaa
    26281 tcacatgtta gttctgtatt tgctactcct ctaactccat ctgttccaaa atactttctc
    26341 actaaagatt ctcctttcat aattgaaaag cttgtaattt gtattatatt tttatagttc
    26401 ataaaatatc attataggct taatttcagt cataaaaata ttttaatact tatcagcaaa
    26461 aaagacaata gaaataattc tattgtcttt tttttgacat ctttttcttt attagttagt
    26521 tgctttagaa catgtagcat ccataccttt ttgtaaagct tctctgttta atggaacgaa
    26581 tttttctttt ctaggtccga aaactttttt gaatgcttct agtacagatt ctatatctac
    26641 agtatcagat acttttaagt aagcgcctaa cattatcatg ttagcaactt tatcatttcc
    26701 taattcagct gctaattcat ttgctggtat ataataaaca tctacatctg ttctatcaac
    26761 tttttctttt attaaagaac tatttactat tatttttcca ccaggaataa catcatcttt
    26821 gaatttttca aatgctggta tatttaataa tatagctgca gttgcatcat ctgttataag
    26881 tggagaacca actggctcat tagatacagt tactgcacag tttgctgtac ctccacgcat
    26941 ttctggtcca taagatggta accaagaaac ttcttttcct tctagcattc ctgcataagt
    27001 aagtaattgt cccatagaca taacaccttg gcctccgaag cctgcacata ttactctagc
    27061 tgtagacatt tacttcacct cctcaacttc aacatttttg aaatttccta atggatagta
    27121 tggtatcatg ttgtctctta accattgtaa agcatcgttt ggagctagtc cccagtttgt
    27181 aggacaagta gataatactt ctactattcc aaatcctaat cctgcttgtt gaacttggaa
    27241 tgctttcttt atagcttttt tagcttgtct aacatgagct ggagtatcaa ctgctactct
    27301 ttctacaaat acagctcctg ttaatgtagc taacatttca cttactctta ttggataacc
    27361 ttgagtttca gcatttcttc ctgattgagc agtagtagct ctttgtccaa ctaatgtagt
    27421 tggagccatt tgtccaccag tcataccata agttgtattg tttacaaata tagtagttat
    27481 tttttctcct ctagcagctg catgaactat ttcagcagta ccaattgaag ctaagtctcc
    27541 atcaccttga taagtgaata ctgtattttc aggatgaact ctttttattc ctgtagcagc
    27601 agctggcgct ctaccatgag cagcttcttg agtgtcacag ttaaaatatt tatatcctaa
    27661 aactgaacat ccaactggaa caacacctac agcatcacct aatactccta gttcttctaa
    27721 tacttcacca actaatctat ggattatacc atgtgtacat cctggacagt agtgagtttg
    27781 agtatcttgt aatccttcag ttttcttaaa tacaacagcc attatctagc acctcctgca
    27841 accaattctc cggcaataat ttttttagct ttttcaacta tttctgctgg tgttggagtc
    27901 attcctcctg gtcttccatg gaagtaaact ggtaatttac cttctactgc cattttaaca
    27961 tcttccacca tttgtcccat actcatttca acagtcaata tgtttctagc attaggaatt
    28021 tggttgaatg cttcgaatgg gaatggccat aatactttag gtcttataag acctgctttt
    28081 attccttctt ctcttaatat atcaattgcg ctttttacta ctcttgaagt agttccataa
    28141 gctgcaaata caaattcagc atcttctgtt ttgtacattt cgtattgtac ttcattcttt
    28201 tccattgcat caaatttttc ttgtaaatgc caacaatggt cttctagtac ttctggctct
    28261 aagtatagag agtttattac attaggtttt ctttttcctt tagttccaac agttgcccaa
    28321 tctttaggag gcaattctct tttcttctca ggagctctaa attcaactgg ctccatcatt
    28381 tgacctatca taccatctgc aactaccata actggtgatc tgtaataatc tgcaacgtca
    28441 aaagcttcca ttatcatatc aacagcttct tgaactgtag ctggtgcgaa tactggagtt
    28501 ctgtagtctc catttcctcc acctcttgta gacataaagt agtcagcttg tgaaggttgt
    28561 atacttccaa gtcctggacc acctctcatt acattaagta caacacaagg aacttctgct
    28621 cctactgcat atgttatacc ttcttgcttt aaagcaactc ctggtgaaga tgaagaagtc
    28681 ataactcttg ctcctgcacc tgctccacca taaaccatgt ttatcgcaga aacttctgat
    28741 tcagcttgaa cgaacgctcc acctatttta ggtaactctc ttgataaata ttctggtaat
    28801 tcactttgtg gagttattgg gtaaccgaag aaatatttgc aaccagcttc tatagctgct
    28861 tttccaaatg cttcgttacc tttcataagt atcttagcca ttaaaattcc cccttaaata
    28921 ttaattaaat taatctcttt ctactgttat aactacatct ggacacatag ttgcacaact
    28981 tgcacatcct atgcattttt ccatttcaaa aacagttgct gggttatacc cttttttgtt
    29041 tattacattt gagtctaatt gtatgatttt tactggacat gcttcaacac acaatccaca
    29101 acctttacaa cgttcttgat taaaagatac ttttccttta gccatttacc taagcctcct
    29161 taaactttct atatatttga gctcaaacta catccagtct tctctcatat ataattttat
    29221 aggaaaaatt tctccttcta aatcttttgg taactgttca acaagatttt ctaaacatgc
    29281 aacatatctt atcggtatat tacatttttc agaaacttct tttgcaactt tctgacctct
    29341 taaaacatca tcaatagtag taaacctaat aagatgagta ttattaatta gacctgttac
    29401 ctttaattta gaagattttt ctatttcttt tatatattgg ataacttctt cagaagtttg
    29461 agttttctcc ctatttgcat ttattacaaa aagcatatca taatcgcctt cttttaacaa
    29521 atgactaaat cttcctataa ccctagcacc tacattatct ccacctaaat ctataacaga
    29581 attgtatgaa tggtctacca taggagacat tacctctgct gatactgctg gtaaatctaa
    29641 tgttggagca ttaattgagc tatctattgg ttgtatacct aaggatttca ttaactcttt
    29701 cttttctcta gttctaaagt atacatttac cacatctaaa tcggctatag caactttgcc
    29761 agaaaccata tctcttaact taacaacata gttcatcgca aattcacttt taccacttcc
    29821 atagtgacca gttatgattc ttattctttt atcattagtt atcataaact tgagcctctt
    29881 cttcaccagt taaaactcta agtccacctt gagctaatgc aatcatttca tcttcacctg
    29941 gataaacttt tacatctgct ataaatttaa ctctatctgc aatcatttct gtaaacattt
    30001 ttgaatatgc gattccacca gttaataata ttgcttttac atctccctta agaactgcag
    30061 cactagctcc tatttcttta gagatttgat atgccatagc ttcatatgct aatttagctt
    30121 tttcatcacc agcttcaatt ctttcttcaa cttctctagc atcattagtg tttaagtatg
    30181 caactaatcc gccattacct tttattttct ttttaatttc atcttgagta tattttccac
    30241 taaagcacat ttttactaat gcacctactg gtagtccacc acttctttct ggagagaaag
    30301 gtccttctcc atctaatgcg tttgcaacat ctactatttt accattttta tgagctccaa
    30361 cagaaactcc tccacccata tgtgcaacta taagatttat atcttcatat ttcttgttta
    30421 tttctctagc atatcttctt gctattgcct tttgatttaa agcatgtact acacttgctc
    30481 tacttatttc aggcatacca gaaattctag caacatcttc taattcatct acaacaacag
    30541 ggtctactat gtatgaagga acatttactt cttcacctat ttgttttgct attattccac
    30601 ctaggtttga agcgtgttct cctaaaactc ccacttttaa atcttcaatc atagcagcac
    30661 ttactgaata agtaccacct tttataggtt taagaagtcc tcctctacct actacagcat
    30721 ctaattcaga tgtttttact ccaccttctt ttagagcttc ttctattact tgtttacgaa
    30781 attcaaattg gtcagacacc ttctcatatt ttcctatttc ttctgaagaa tgtcttaaag
    30841 ttttttcaaa tactaaatcc tcattatcaa atacagctat tttagttgat gtcgaaccag
    30901 gatttattgt taagatttta aatattttgc tcatttgcct atttccccct taaaattatg
    30961 cctttgctgc cattaaaaca cctaaagcta ttgagtttag tttagtttct tcactgtctg
    31021 ctctagaagt taatattatt ggtgctttag ccccaactat aactcctgca ttttttgatt
    31081 ttgagaagaa taccaaagct ttatataata tgttaccacc ttcaatatct ggggctaata
    31141 atatatcagc tcttcctgct acaggatgat ttataccttt atgtttagct gcttctaaag
    31201 atactgcatt atcaattgca aaaggtccac caaccataca acctttgatt tctcctcttt
    31261 catacatttc ttctagttct ttagcttcaa ctgtatcttt catttttgga tttacttttt
    31321 cttttgcgca tattgcagca acttttggtt cacttatatc taatgaatgt gctactgtgc
    31381 aagcattttc tatgatttgc tttttagtat ttgtatcagg agctaagttc atagctgcgt
    31441 cagttacgaa aaataatcta tcatatccct ctacatcaaa tactgctacg tgacttaata
    31501 catttccagt tctaagacct acttctttat ttaaaactgc ttttagtatt attgatgtgt
    31561 ctactaagcc tttcattacc atgtcggctt ttccttgtga aactaattca acagatttta
    31621 gagatgcttc tgctaaatct tttatatcta tcagttcata attttcgata tccatgtcta
    31681 tgctttttgc aatttcttta gtcttttcta tatctcctac taaaatggca tttgctattt
    31741 tttcttttct agccatttca actgccatta aaacttcttt atcttggcaa catgctactg
    31801 atatagtttt aggtcctctt tcttttgcaa acttaattac ttcttcaaaa cttctcatgc
    31861 atttccacct catcttgaat tttttgaaat agaacttttt atatctcaat taaattataa
    31921 ctcgttcagc aacgttttcc tagagataat tgaaatattt aattcaaaat atttatttaa
    31981 atctatttat cagtcttttt tcatatatct agcgcttttt agatttaaca aatatctcat
    32041 tgtccttatt ttataatata actaaaataa acaagatgca acaaaaacat aaaattaaca
    32101 aacttaattt tataggtttt tttgtcgcat cttgtaacaa ctaaatattc gaaatatttg
    32161 ttcattccac ttcaacaata ttaggttcta tatttatttt tttaaattga tatgttgttt
    32221 catattttat tttatattta ctatcttcct caggaccttg tgataaatca atatataatg
    32281 aaatatcttt tttatttaac ttactcaaat cctccattga ctctacaaca acttttatat
    32341 ctacatcaga aattacattt ggattatcca tatttgttgg gatatttctt aattcaatat
    32401 ctcccgcatt atataaaaat tctgcactca attgttttgt atcatttaat ttaattgtta
    32461 tagagctatc ctcaagagtt ataccctctg gaatttctaa gctaacttct ttttcttcac
    32521 cattagccaa atcagctaag tcaataggtt ttgtcttaat ataacttatt ttgtctatat
    32581 cttctttctt tccttttatt gtaacttgtt ccttacttaa tttatagttt ttcagtacat
    32641 cattatcttt attatcattt agacttactc ttattgggac aattttttcc ttcaatagtt
    32701 ctatctttgc agtcacaaag gatgaactta atgttactcc ttcaacctta gttccatcac
    32761 tatttaatgg tattaattgc aattttttag aaaaatcatc tgtctcatta ccaacatcta
    32821 gattagctct tacacttttg acttctttaa ctagagttct tggacctgat acatcgacaa
    32881 attccttact aagttcaatt ttatttatat ttctttttga atttccactt acttctacct
    32941 ttatatctct tttttctttt atgttttttt caagatttac tactaaaaca tcatgcttaa
    33001 attctagagt aacaccttga ggaatatttg cttttaaata tacttccttc tgtccttgta
    33061 ttgggtcttc aatggttcca tatacatgta tatcttcttt tttaacactt tgtacctttg
    33121 ataaactacc ttttatatat acatctgctg ttaatttttc gtctggatat ataacaagat
    33181 ttttatcctt taaatcactc atattactta tagatacagg tatatcttca aaccctttag
    33241 tttcttcagg gtcaactaca gccataacat acatccataa tgcaattgca cttaaaaatg
    33301 atattatttt tattttggta ttatttttta gtctatttat catttaaata taccaccttt
    33361 gaaaaaactt cttgtttctg tatttgtttt taaattacta cgtaatatat tcatcattct
    33421 ttctctagaa atatctctat ataacttacc tgctttagca attgatacgc cacctgtttc
    33481 ctctgataca atcaaagtta tacagtcaga cacctcactt acccctattc ctgctctatg
    33541 tctagtaccc aaatctttac ttaaatcctt actttctgtc aatggcaaaa aacatgcagc
    33601 tgcttttacc ttagaatctc taattacaac agctccatcg tgtaatggtg tatttggtat
    33661 aaatatattt attaatagtt gcctagaaat ttcagcatca ataatagttc ctgtatttat
    33721 tatatcactt attctagttt ctctctccat tatgataaga gctcctattt tttgtctaga
    33781 aagtgaatat aaagcttcta caatttcttc tatatttcta ttgagagttt cttcagaagt
    33841 atttacattc tttccaaata aattaaactt tgctcttcct atatgttcaa gtcctgctct
    33901 aaattctggt tgaaaaatta taagagcagc tataactcca taatctaaag ctttaagaga
    33961 tatccaataa acggtatgca atttaaaggt gttactaagt tgagttgcta ataaaagaaa
    34021 tattatacct ttaaacactt gttctgctct tgtatctttt ataaacataa atattttgta
    34081 aaaaatatac gctactatag atatgtcaat taaatcatat atactcattc ttaatattat
    34141 attaaaaaat ccatttaaaa aagaatttat atctaacaca gttttatcct cctgtttgat
    34201 aacttcttaa ttatttacac taagtattat actatataaa tctaactcta tacaaactat
    34261 ttaactttta tgtaaataat tctagtttta tatttggaga ttgttaaata atctctaaac
    34321 acattaaaaa tatcaactta ataaatacat atttcttatt aaagttagtt tttactcaaa
    34381 taactcaaac catcaaggcg cgaatacaaa atagtactcg cgccttaatt cagttttatg
    34441 ttctaaaata tcttaatata tctttgttta atatatatta attattataa attctataat
    34501 ccacttatta actataaaca aatacaaaat ctaaatatta cttatcatca atattattcc
    34561 attagattca tttttatatt aaagtaataa cttatttcat atctagatta gataaattta
    34621 ttttaatatc ttactataaa ctatcattaa aacttattat gtataactag atataatctc
    34681 ctacagtgaa aataatacac aatacctaaa gttatatatc catttcacag tataataatt
    34741 tatttagtat aacttataaa tattacatta aattgtaaat tcactatagt ctctaatttt
    34801 ttataatttc ttatacttat tgtttagaca aaaatatgct catgaatctc tttataaata
    34861 atttcataag ttatttcaat atataaatac cataaagtat acaatataat gtatataact
    34921 cttactaatt agttatattt attatattta actgatgcct tcataaacat cttgtagact
    34981 attatacatt accctccgta tgtttacaat attaatgaat atactgtaac tctattagaa
    35041 tatactgtaa tttttacata gtttaaagtt tataaataaa aagacacagg taatatacat
    35101 gtttcctgtg tctttttaaa caatataaaa gtttactata atttatagta aacttttctt
    35161 aatgagcgca cagggattcg aaccccggac acacgcctta gaagggcgtt gctctatcca
    35221 gctgagctat gcacccaagt tattatggag cgggaaacga gattcgaact cgcgacttca
    35281 accttggcaa ggttgcgctc taccactgaa ctattcccgc ttatttaatt tggcgacctg
    35341 gaaggggctc gaaccctcga cctccagcgt gacaggctgg cattctaacc aactgaacta
    35401 ccaggccgcg aatggtggga ccaacagggc tcgaacctgt gaccccctgc ttgtaaggca
    35461 ggtgctctcc cagctgagct atggtcccat ttttggagcg ggtgaagggg atcgaaccct
    35521 cacagctggc ttggaaggcc agaactctac cattgagcta cacccgcata tttaattgaa
    35581 ttggtgactc gtaggggaat tgaacccctg ttaccgccgt gaaagggcgg tgtcttaacc
    35641 tcttgaccaa cgagccgttt taatggttgc ggaggcagga cttgaaccta cgaccttcgg
    35701 gttatgagcc cgacgagctg ccaactgctc caccccgcga tattgtttta taagtgccga
    35761 agaccggaat cgaaccggta cgagaggtaa gtcccgcagg attttaagtc ctgtgcgtct
    35821 gccagttccg ccacttcggc ttttttggct ccaagggtgg ggctcgaacc cacagcctac
    35881 cggttaacag ccggttgctc caccgttgag ctaccttgga acattt
//
LOCUS       DAYAPA010000030        35507 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.030, whole genome shotgun sequence.
ACCESSION   DAYAPA010000030 DAYAPA010000000
VERSION     DAYAPA010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 35507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35507
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.030"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(53..934)
                     /locus_tag="ACGI59_001359"
     CDS             complement(53..934)
                     /locus_tag="ACGI59_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HFG9739152.1"
                     /translation="MNKIKIKQVDAFTTIPFGGNPAGVVTDTSNISDEIKQNVAKEMN
                     LSETAFVSESNIADFKVQFFTPGLEVDLCGHATIGTFHALLEEGKLNTNKSIFYQETK
                     AGVLSVELKKINNENIFMMEQNTPEFENLDNCRKELAQMIGVHEDRLLEYPIIKVNTG
                     LWWLVFGLKSLDDLKNISPDLNKIKKFSGDNNIIGITPFCIETIDKKCDYHLRSIAPY
                     VGVTEDPVCGTGNACVSSYIVHHNILDKTSFIGEQGNFINRPGKVYVNITRENNNITS
                     VKIGGSAYTVLSGEIMY"
     gene            complement(960..1673)
                     /locus_tag="ACGI59_001360"
     CDS             complement(960..1673)
                     /locus_tag="ACGI59_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFG9739153.1"
                     /translation="MDNIINELQNIGFTKYESQIYISLLKESPLTGYEISKLSGVPQS
                     KVYENITKLLNNNIIINVGTDPIKYIPINPNELLKNKKKEFDNSMDNLKNSLQILNKS
                     KSVEYVLNIKGDKNILKKAIEMIKNAKQEIIISCPYEEILELKKELLIAYKKNINIEI
                     LLLENKSLKGLDNIHIHGGNKTTLENKMLYKGIILIVDNDEILTGNLSEGSEAISIWT
                     KNSNILYIGVQYIKHEIYI"
     gene            1931..2257
                     /gene="trxA"
                     /locus_tag="ACGI59_001361"
     CDS             1931..2257
                     /gene="trxA"
                     /locus_tag="ACGI59_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HFG9739154.1"
                     /translation="MYIMVKIVNNNEFINEVENKDGLVVVDFFATWCGPCKMLSPIYE
                     ALGNEMVEKANFLKVDIDQSIELAQKFEVSTVPTMLIFKDGKPVDRLIGFMPKENLKN
                     KIESYM"
     gene            complement(2464..3738)
                     /locus_tag="ACGI59_001362"
     CDS             complement(2464..3738)
                     /locus_tag="ACGI59_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HFG9739155.1"
                     /translation="MQFMDVSKYIEGANLKVKTKSGETVKRIYFNNSATPLVLKNVVD
                     NLNCEIPLLTYINAPGIISEKNTLKYENVRSTILKLIDGDEEKDSVIYVKNATEGINF
                     LAKFFKDENPDKFVITTAMEHMANYLPFKVNFPTKVVGITEKGELDLCQLENILKNYA
                     GNVSLVTVTGASNVTGITTPIYEIARIAHKYGAKILVDIVQVIQHKPFSMMPHECDEH
                     IDFVVFSAHKCYSPLNGGALVGPKKFFEKFNPVLYGSGSTKFVNDNKILYANVPQRFE
                     AGYPEYFGTLAMGKALNTLNKIGLNRISKYERELFLYAKEEMSKIPNIIIYGDKSNNV
                     IIPNISFNISNMYYKDVAKYLVDNFGIETGAGVVGADIYVQKLLGISPKEAYVRFMRE
                     NPVGLVRISLGMYNTFDEVDSFIHAIKTLAFK"
     gene            4619..5455
                     /locus_tag="ACGI59_001363"
     CDS             4619..5455
                     /locus_tag="ACGI59_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFG9739156.1"
                     /translation="MNIKKIFNNNVVVSSLEDGTEIIVTGAGVGFKKKVGDLIDENLI
                     SKKYFVQDDQRDKYNQILNKTSIEYFKISEEIIEKANEVLNTQVNDSIILALTSHIEF
                     AVQREKQGIKLPNLILNETKQLYREEFEFGLWAIDEIEKRIGIKLPEDEAGYIAIHII
                     NGLENSQKDEGINILEFSKQVIQIIEEVHGFKLDVNSLNYTRLITHLKFFVQRILRKE
                     TYNDSDIEDMYKLVNKNYNKPKVCTEEIAALVLDRFEYKISKEEELYLMIHINKITNS
                     GC"
     gene            5808..7385
                     /locus_tag="ACGI59_001364"
     CDS             5808..7385
                     /locus_tag="ACGI59_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFG9739157.1"
                     /translation="MKKNKLGLWNFFQMLGKTFMFPIALLSVSGMMLGLGAGFTDPKM
                     IEMVPFLGNEYVNLVLNFMLTIGLFAFNNLGALFAMAIPLGLLKKEKEFGAFSGLVGF
                     IAMHIGTNFYLTRADLLVPAEQMSTNGQAMIMGIQTYNTSVLGGIVAGLIVYAIYDKV
                     SNLKIPESLGFYSGPRLVPIVSLIVMSIVGLLIPIVWPPFFNAFQSLGRWISSAGPLG
                     YFSYAVAERVTIPFGLNHLVTSVFRFTPIGGTAVIDGETYFGTLNMFMAYVENNQIIP
                     LDLAGKMEQGKLMIQYGLAGAALAIYRTAKPENRKALKGLLISGVLTVIIGGISEPIE
                     FLFLFISPALFAFHTFMNGLANMVLPYLGVLMGFTGDLIAFISFGVLRGTATGWPIAV
                     LVAVLYFAIYYFVFKWAIIKFNIKTPGREDKEIKVSEGNGSNFKNLSTYKGQQMISAL
                     GGQSNILSLDNCVTRLRLKLQDVSLINEDAIKEAGGIAVVKLDEHTIQVIIGTQVYSL
                     RKQMDKAMEGHLEACND"
     gene            7378..8562
                     /locus_tag="ACGI59_001365"
     CDS             7378..8562
                     /locus_tag="ACGI59_001365"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFG9739158.1"
                     /translation="MINFDKKVDRLGTYCTQWDYVEDRFGKKDLLPFTISDMDLEFPF
                     EITEVLQERLNHRVLGYSRWKHDDFRNSVTNWYIKRFNCSVDSNWIYYSPSVMYSISK
                     LIEMLSNEGDGILINTPAYNAFYDVIINNNRNIIKSHLVNRNGFYSIDFNDFEKKCKK
                     SKIFILCNPHNPTGRVWSELELVKMIGICKNNDVKIISDDIHMDIVYDNRMTPILNVA
                     KDYLDSVFICTSASKSFNIPALTGSYVIIPNEGIRNKFENITRYRDFVNSPAILAILA
                     TMVCYDDCEYWLDELLKYLKENLKYTIDYIKDNLEPLKLVMPEGCYFAWIDFSELKIS
                     GEKFQKLLMDIGEVAIMSGSVYGEEGEFYLRLNVACSREKLEDGLNRIKKTMDYIEKR
                     IL"
     gene            8580..9179
                     /locus_tag="ACGI59_001366"
     CDS             8580..9179
                     /locus_tag="ACGI59_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFG9739159.1"
                     /translation="MGYLKTMVDVMTTEMNAIRCLIDEAGIEYESIVSEIANCEGKVI
                     FMGVGKSAHIGKKLAATFASTGTPSFFVHATEAVHGDLGMIESKDITILISNSGNSME
                     VVNCIKYIKAIGSKTIAFTSNRNSVLAKECDYALIYPAKDEADHLNLAPTTSSTITLV
                     LGDSIACALSKSSNFGSSDFYKYHPGGSLGEKLKTANNG"
     gene            9203..9673
                     /locus_tag="ACGI59_001367"
     CDS             9203..9673
                     /locus_tag="ACGI59_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFG9739160.1"
                     /translation="MFKMFKKKSIKSPISGKVIELSKIQDGVFSQKIMGEGVAIDSTG
                     DIVYAPINGKIVVVAETKHAFVIELENGMELLIHVGLDTVNLKGEGFEALVSMGEQVK
                     EGTPMLKINRPLIESKGISLITPVTITNHSEYNMNTCNVGNSVEGGKDTVIEFK"
     gene            10032..10778
                     /locus_tag="ACGI59_001368"
     CDS             10032..10778
                     /locus_tag="ACGI59_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HFG9739161.1"
                     /translation="MKIENLSIDDIKRGYIFNDDLNCYSCIKCTKTYEVGEIYKIGNR
                     FFEASKAIELHMELEHSDYLEQLLNSDSKYNTLTENQKQLFQLFISDLPDKEIAKQVG
                     VSTSTIRHQKFIFREKAKQAKLYLALYESVFEDKSNKSSTIIPIHEHARMVDERYVVT
                     EEERKHILDTSFESMNPLKLKVFSSKEKKKVVILSKILEQFERGKKYSEKEVNQILES
                     IYDDFATIRRYLIEYGFMSRNKECTKYWLT"
     gene            10839..11513
                     /locus_tag="ACGI59_001369"
     CDS             10839..11513
                     /locus_tag="ACGI59_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFG9739162.1"
                     /translation="MNDELKSILYNKGVDIVRFVDISEFPINQTQGFSKAILFCIGLS
                     KKFITDIYNNLPTDSDEFLEKEEKAEELADWISEYIQNKGYRAYSQSEKNNLEHGYFE
                     KAYINPEMQSGISPLPHKTIANISGIGFMGKNNLFVTEEYGCAFSMCTVLTDAPISVE
                     RYPLIDSKCMDCNVCVENCPAKAIHGNEWTLPGKRESIIDVSKCFCVLKCMMSCPWSL
                     RYANQK"
     gene            11881..13227
                     /locus_tag="ACGI59_001370"
     CDS             11881..13227
                     /locus_tag="ACGI59_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 55 protein"
                     /protein_id="HFG9739163.1"
                     /translation="MEIETKTPDWINVLELNSKIDITGKYDVSNIIQDIVSDKSLEGS
                     IIYFPKGNYLFEKGIKISQQITLQGDSYYGGDNQVSNLDKKQSIIGTTNFITRGVSNM
                     SIIALTGTSQCIKNINFYYDSHDIEKIPPKNVSAITEYGETQGLSHFEHLFISGFSGI
                     GIEIPYYSTGNDIIVSSCGLGMRLGEKSMLSSSKIYECKNGMEITTGVSLNNVRIEEI
                     KEIGINNKGFGFNLIMNLMVDQCGYCGFMFEKMCYSQITARITRCGQYYKSVDYNAYE
                     NMEDRKEKAYSIFYGDILESCNIVLMNRNIDTLESDSLNEHKIYVIKANETNNVMLTC
                     YAEADNFIQCAKGNLLLENGENTYKFYNGEICSVGGVQISDIDEKDLIKIIENTLYID
                     DKGKYLMVPRLKKNTVIYSVLDSTEEINKLHGGIWEQIDVKVLAGESMYYYKKIKD"
     gene            13254..14654
                     /locus_tag="ACGI59_001371"
     CDS             13254..14654
                     /locus_tag="ACGI59_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HFG9739164.1"
                     /translation="MEESIIIKDISGWINVLDYGICNDETKDVSFKLQEIMDAVPEDS
                     IIYFPKGKYLFESGVKIKKRLTLCGDSYILANVPKNTAGVTQFTFKGKKNPKSENRAI
                     ITAKSVRHCIKNICFYSDSCDRDMTSNHEPPTDGKPRFHHNVIINYRNISAVICEDKQ
                     GEPGHYENLSFSGFSGIALDMPNNSTANDITIFTSGLGISTGENSTIANSKVWGCVDG
                     MIISTGTFINGMRVEEIKKVGIKNIGRGLNFITNITIDQCGYCGVWFDSISNVQISGN
                     ITRCGQYYYDVSYDNYLMLDNRVKEAYSLFYGNLLESSNIVLSNTNEDNWEDFKEDKH
                     KVYLIEALKTKNVLLTCNIDATDWIVKSEKGNLTYNNMRQTVIFYDGKLSSINGIGIS
                     DEDYGDKVKIKKGNIYINSNDKDIKMHKPEVGDIMSTIIKDKSILSAHYGGTWEKIGE
                     KIEFGITVHYYKLIEK"
     gene            14864..16300
                     /locus_tag="ACGI59_001372"
     CDS             14864..16300
                     /locus_tag="ACGI59_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 55 protein"
                     /protein_id="HFG9739165.1"
                     /translation="MEGRTGIKDGRWINVVERGIKNDGSYNIGPALQDIIDEIETDFC
                     VLYFPKGKYLFTSGVKINKEIVLQGDSNSSTGTTQFITRGVENMSIITLTGKKQCIKN
                     INFYSDSCEIQVNKEPPTKGNPKYHYEMIINKNEKNVEMNNVSAILYDNSKNMIKGLG
                     HYENIYISGFSGTGIRIPYYSIVNDITVSSCGLGIDTGIDTIISNSRISKCQNGMRIR
                     TGTSINNVRIEKIQKVGLISSQVKGYEGYGSYKINNITIDQCGYCGFSFDSMKDSYFS
                     GIIRRCGQYYYNTDYDTYLSIKDRVEEAYSLFYGNYFTECNIDLVSDNKDNWDDGLEY
                     KHKVYVFKTLQIPKLTLRCNIDAYDYIVKSVHGGGNLMLKNNFNNYIFIDSSNPEVIN
                     GINFNYQKNGNLLRLNNGKLSYKNGKEETAIFRPNYGLIVASTDSECEQIEATYGEKW
                     EKLGSEVIGEETVYYYKHHEPDKTNQIQ"
     gene            16644..17588
                     /locus_tag="ACGI59_001373"
     CDS             16644..17588
                     /locus_tag="ACGI59_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1848 domain-containing protein"
                     /protein_id="HFG9739166.1"
                     /translation="MIVSVSRRTDIPAFYSKWFFNRLEEGFVYVINPMNPKQVSKIEL
                     NPHTVDCFVFWTKDVTPMLYDLNKLRDYKYYFHYTITSYGKEVETCILDKRKVIDSFK
                     ELSRKIGKERVILRYDPIFLSEKYTVDYHIKAFASLCNQLDGFTEKCIISFIDLYKKT
                     KFNTKALHIKPIEITEIETLSKEISKIAHKHGIILEMCSEEYNLSKFGIGKSKCIDDR
                     LISKIIGCEVEVKKDSTQRDICGCVKSVDIGQYNTCRHYCLYCYANFNYAKVEENCRL
                     YKEDNKLLIDTLRDDAKISIRDMKTIKVDKNKQISIFD"
     gene            17757..18689
                     /locus_tag="ACGI59_001374"
     CDS             17757..18689
                     /locus_tag="ACGI59_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HFG9739167.1"
                     /translation="MNLLKKYPIPIASLILSIFTLGNLLQSYSESLRNVIGFIGFILY
                     AIYIVKIILLRKGVKEQLENPLISSTFPTITMATMIFATYIKPLSLELSIMIWSIGIV
                     GHLVLIVLFSKKFLVKFSIKTVFPSWYIVYVGIVAASVTAPAFNQLLIGKIAFWIGFV
                     SYIVLIPVILKRVWIIKEMPEPSLPSIAILAAPGSLLLSGYINSFSDKNNVVLYLLFI
                     LSIVFYIIVLGYLPKLLRLPFYPSFAAFTFPMAISAMGMKLMNGYLTKSGHFISWISY
                     ASKLEEIIATVIILYVLVLFIIYLSKSETTSKAK"
     gene            18794..19261
                     /locus_tag="ACGI59_001375"
     CDS             18794..19261
                     /locus_tag="ACGI59_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HFG9739168.1"
                     /translation="MKTKEEYLKEVRNISEEYFRKGEFFCSEAVLQTINDALGQPLSP
                     EITKLASGFPIGLGKAQCLCGAISGGEMALGIVYGRVHGEAMNPKMFEHAKGLHDYIK
                     KEYGATCCRAITKKWDGDNFMSPERKEHCIKITGQVTEWVADKLIEDEKLNIK"
     gene            complement(19505..20359)
                     /locus_tag="ACGI59_001376"
     CDS             complement(19505..20359)
                     /locus_tag="ACGI59_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFG9739169.1"
                     /translation="MLTIIIALLPVMGWGLMPIVANLKKSSPHEQLLGTSISAFIFST
                     IITLVIHPEITFFSFCISMISGIFWSLGQLMQFKAIQIASVSKVMPISNGSQLTFTTL
                     LAVILFNEWTSSKMLLIGSLSILCIILGILLTNYQTKKSTDKNMLKCVGVVLLSSLFL
                     SLYVVTNQIFLIEGYRIILPQSVGMLITSSIIVKYFSKEIVYRENVLFNLITGILWSI
                     ANLGMFITTNTLGVTISFSISQSCVIVATLGGIIFFKEKKNKKEWLAIFIGIILIMSG
                     VFMLSIIK"
     gene            20525..22489
                     /locus_tag="ACGI59_001377"
     CDS             20525..22489
                     /locus_tag="ACGI59_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFG9739170.1"
                     /translation="MLTDMIQNREIKIIKFLLKEGHTTVKAIADDLDVSEKTVSKSLK
                     EVEAVLKKIGLSLIRKPKIGVYIDGDLKKILPYLDNKGSQIPTTREERVIYIYSVLLK
                     SNDYITIQQLSDELYISRGTIERDLAEVEKLLKNEGISIYKKPSKGIKLNISERDKRQ
                     LTAKFIHKFWSRNWYIKQEQESFYQAFEKIQADVNGIFSEDDLSIIIDILRNFSRERK
                     FEFSDYEFQSLVIHLAIAVERIRKGEYIEESLYTGNIENNQMSNTNYLAKQLEEKFSI
                     LLPKSEIGYINLHLVASDQHRDTNELKNTSTNPCETSTNNISNLREIIKNTLYETAYD
                     KDLIDGLTSHVHSSINRLKYGLSIKNPYVNTIKQNFSLSFEQALDLKKVIEFIYDIRI
                     NDDECAYIALHFEAFRERMKELPQNIRVILVCSTGLGSSRLLSARIKKYFPMIKIEKV
                     LSVQELVSSKIDADLVISTIYLELETIPTIVVSPILNESDIRTLERNIQNVSSYTSKK
                     YKSFRNLIFEENILLNIEASNYEDAIKYITDNLIKKGFAKEGIAKSAIERENLSYTSF
                     QSIATPHANPSYIKKSNISVATLKNPITWGNEKVEVIFFISLENDKNLELDEIYEVFY
                     DFIDNKKNINDILSATSTKEVYDLLVEESI"
     gene            22489..22950
                     /locus_tag="ACGI59_001378"
     CDS             22489..22950
                     /locus_tag="ACGI59_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFG9739171.1"
                     /translation="MKISDVLKKEQIVFNLDTNTKEETIKKLSSIFVENGIVDNEEEY
                     VKSVLEREEHSTTGIGNGIAIPHGKSNAVKKSSIIFAKTKHKIEWDALDEKPVDSIFL
                     LAISNIDKDSNHLVLLSKLATKLMDDDNVDALKLANSEQEIINIFSEEGEM"
     gene            22953..23273
                     /locus_tag="ACGI59_001379"
     CDS             22953..23273
                     /locus_tag="ACGI59_001379"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFG9739172.1"
                     /translation="MKRKIIAVTACATGVAHTYMAAQALKKASKKMGHLIKVETQGAT
                     GIDNELTTKDAQIAEVVIFAVDTKVRNAERFEGKEILEVPVNAPIKDAEKVINDALKL
                     IDTK"
     gene            23289..24389
                     /locus_tag="ACGI59_001380"
     CDS             23289..24389
                     /locus_tag="ACGI59_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFG9739173.1"
                     /translation="MLKKIVSELKKHVLTGISYMIPLVIAGAMIMAISRVGGSFYNIP
                     DIWDAKYAESASSIVRLLHDLDGFGGTALGLMFPVIAAFIGFSIADKLAIVPGLVGGM
                     IAKDIGAGFLGALAVGLIAGYTCLFIKNHVKLPKSAASIVPIFIVPVFGTLITVVLIN
                     YVIGIPFATLNTGLENWLNGLSGTNQILMAAIIGAMMAVDLGGPVNKAALTTSLALLT
                     SGIYAPNTAAMVGIVIPPLGLGLATILAKQKYNKQLREAGKSSLIMGLIGVSEGAIPF
                     AVESPLKVIPSCVIGTAIASAMAVGLGSVNATPISGFYGWFTVENWPMYVLSIAVGTV
                     IVAGLVIVLRGNPVIEDDEFEDDDFDEAEWES"
     gene            24509..27109
                     /locus_tag="ACGI59_001381"
     CDS             24509..27109
                     /locus_tag="ACGI59_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFG9739174.1"
                     /translation="MIKAHIVNHTHWDREWYFTSGDALVLSEQLFTDVIDELERNPDV
                     SFVLDGQLSILDDYVQLHKEKIEIIKKLIKEDRLHIGPWFTQTDAFFARGESILRNLM
                     IGIFESKKYGKYMKIGYLPDTFGFNAQMPILLKHVGLDNIFFWRGIHLGKQVQSPYFK
                     WKSLNGESYIYAVNMPHGYGTGMLLEPSLKYVNGRLDPAIDFIKSYTDVDEVLIPSGN
                     DQLNIIGDFKNKIKAINDIGKYSYITSSYQEFLKYIKSIESLESYRGEFREPVLARIH
                     KSIGSSRMDIKLACDKLENKLIKRIEPLLVIAKKSKIEISNQLLINTWKKLLEGQAHD
                     SLAGCVTDTVTDDILHRIREANEICDSIENTIVKKISEGLKLSKNDILVFNTEAKRFN
                     GYKEIQVVSDSKNIYFKDNLDATIIEETYVKPRENVLEETPAGNIFIEEPGYYILKVR
                     INVQLPALGYKVVSFESSDKEMVSLNKSNDAFISNDNYKIIYEDGSLNLQLKDGTYIS
                     NFLTLKDSGNAGDTYDFSPLKNDEDIKLSFDKVYTEKALGYEKMVVKGTTLLPLTLED
                     RKNKYLNGKLNVKVSIVLSKENPLMDVKLCVDNTIYNHRLRVHIKTDIKDNKNIASLP
                     FGYITRENGVLDNWEDIYSEMPIDLEPLESNITLTNQNRSCTIFTKGIKEYQHIEDEI
                     ALTLLATTDELGKPDLLYRPGRASGDTTKKGHIRIKTDKAQMLKKLEFSFAIYMDSKS
                     FDELEIANLTHNYLRECVNYQLQDYNFFLYRIDNKIQKSIVNKTVSRELEIISLPENY
                     LISACYPSYYSKDKFIIRVENPSSNKMILDEQIFKNRNGKIVNAIEDVEEEQVYEIAP
                     FDVISILLDL"
     gene            27578..27913
                     /locus_tag="ACGI59_001382"
     CDS             27578..27913
                     /locus_tag="ACGI59_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFG9739175.1"
                     /translation="MYNIDDMIYNCPVEALSNILGKKWVAIIIWKIQEDKKRFGELQR
                     AIPDCSKKMLVQQLDFLIEQGIIVNQKKLVNNVVESTYFLSESGLRLLPVMEKMIMWS
                     NKNLNCDKY"
     gene            28050..29327
                     /locus_tag="ACGI59_001383"
     CDS             28050..29327
                     /locus_tag="ACGI59_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein/redoxin"
                     /protein_id="HFG9739176.1"
                     /translation="MQNVNLFLVFIEGIVSFFSPCILPILPIYLSILSNSSVENLKEG
                     KTSFIGSSLFKNTIFFALGISTTFFILGSSVKVLSMFFNENKDLIMFIGGIIIIIMGL
                     FYMGIIKSSILNREKRFNVKFKEMKAITAFILGFTFSFGWTPCIGPILASVLVMVSSS
                     SNHLSANLLIAVYTIGFILPFIITAMFYSKLFKTIDKIKSNMEIIKKIGGIILIVSGI
                     LMMVNGFGSISKHFNTSQNSKIESKQEENKRENSTDKEENSDGNDSQKDSNNDNNDKG
                     SNDEDRIKSIDFTLTDQYGKTHKLSDYEGKVVFLNFWATWCPPCKEEMPYIEQLYKDY
                     NKNNDDVVILGVASPNLGREGSREHVVNFLKDQGYTFPVVLDEDGALAYQYGINAFPT
                     TFIIDKEGYVTQYIPGAMDKATMASFIENQRNK"
     gene            29448..30836
                     /locus_tag="ACGI59_001384"
     CDS             29448..30836
                     /locus_tag="ACGI59_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFG9739177.1"
                     /translation="MKKTFDAIIIGFGKGGKTLAGDLANRGLKVALIEKSNKMYGGTC
                     VNVACIPTKSLENSANSVKTKNINSWDEVQAEYEKAIDKKETLITKLREANYNKLNSN
                     ENVTIFTGMGTFIDEKTVQVKTENEIYELVADNIFINTGSRPFIPNIKGIENKNIVYD
                     SESLMNLRTLPKKMTIIGAGFIGLEFAGIYSSFGAEVTILNSNNGILPNEDVEDSEEI
                     IKLLAKRNVKIVNNANIKEIKEVSELAIVEYEVDGKSKELTSNMILVATGRKANTEEL
                     GLENAGIELNERGFIKVSETLKTNKEHIWAIGDINGGPQFTYISLDDYRIVINQLFGD
                     KTRTTNDRKNIPNSIFISPAFSRVGLNVKQAKEKGYEVLVAKMPVEAIPRAKQIGKAD
                     GFIKIVIDKKSNKILGASMICENSSEIIHLIQLAVDLEVEYTYLRDRVYAHPTMTEAL
                     NDILSPNMIKEV"
     gene            31282..31920
                     /locus_tag="ACGI59_001385"
     CDS             31282..31920
                     /locus_tag="ACGI59_001385"
                     /inference="COORDINATES: protein motif:HMM:NF037435.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF434 domain-containing protein"
                     /protein_id="HFG9739178.1"
                     /translation="MKSKEEIEWLLNRDYKIDPVVTFVGDRYQFSIRQRDALKRAVCT
                     DEKNIIRQSKRLSLDKIKEDTVNIDGFNLIISIEVALSGGTLVIGSDGNIRDLAGLRG
                     TYKIIDKTEEALNLIGKFFNKYNAQNLKFYLDAPVSNSGNLKYRILEHAKTWGIETEV
                     ELVKNADVVLEKLDRVVSSDAVIVDKCISYFNVARGIIEEYIKECNIINLNK"
     gene            32038..32907
                     /locus_tag="ACGI59_001386"
     CDS             32038..32907
                     /locus_tag="ACGI59_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HFG9739179.1"
                     /translation="MRGWICVKLTKLNIKKYRAPIYKYALANVNLRSAKSTNSSIITV
                     IPQGAKMEVLDEEDDWIKVMYNSQEGYVYKDLVSVSEYAWSNLNLREDKSTTSNIITV
                     IPEKSRVEVLQVDGDWSKVVYDDKIGYVFNYFLSIDGNKPNELDYKYFYTDMIKFVNE
                     NNIKSTSDYLIVTDLRNKYTYIFKKDNGGWGQLYKWQCTIGKPETPTITGIFYISGRK
                     PSFGTDEYSVKYATRIKGGYYYHSVLYDSTGSYIIDGRLGEALSHGCIRLSTENAKWI
                     YDNIPDTTTVIIH"
     gene            complement(33062..34207)
                     /locus_tag="ACGI59_001387"
     CDS             complement(33062..34207)
                     /locus_tag="ACGI59_001387"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HFG9739180.1"
                     /translation="MSIYYVPPINLLGKGCLNEAKDSIKSLGTKKAFIVSDKFLVSNG
                     TVKKVTDILSQIDVDFVVYDEVKQNPTVTNVNKGLKILKSENCDFVITIGGGSPQDCG
                     KAISILATNGGDIRDYEGINKTSKKCLPIIAITTTAGTSAEVTINYVITDEDRHVKMI
                     MVDTNSLATMTVNDPELMISKPSNLTAATGMDALTHAIEAVVANGAYDVTDSTALYAI
                     KQIFKYLPRAVKNGNDIEAREQMCYACFLNGIAFSNAGLGNVHAMAHQLGGLYDLPHG
                     VCNAMLLPIVEEENAKSAPAKFRPIAEVIGMDVNNKSDKECVDFVIDKIKALSEEVGI
                     PKSLKDVGVDNPNFELLAENSMKDACAGANPVFFDKDKIIELFMKIS"
     gene            complement(34540..35316)
                     /locus_tag="ACGI59_001388"
     CDS             complement(34540..35316)
                     /locus_tag="ACGI59_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFG9739181.1"
                     /translation="MTSDNHRPTARILDILEALAESENGYTLTEIADVINAPKSSIFP
                     IVHTLNARKYITIDKNTSKYSIGIRSYTLGSSFFEKRTIFNYIIDEMTNIVNSCSETC
                     QLGILDNNEVLYIGKVDSPEPIRLISYVGKKIPANCTGLGKALLCDFDKEKLISLYPD
                     GLKGFTEKSITDFDVLYDQLLEVQRTQIASECEESMEHLKCLAVPIRKNGCIRAAVSV
                     TLPLFRANDEKIELIKKLLLNAQKNIQSMMEERNEDIVIN"
BASE COUNT        13726 a         3853 c         5931 g        11997 t
ORIGIN      
        1 aatactagct ttagttagct agtattttta tttgttttta ttaggtatgg gattaataca
       61 taatttcacc acttaagaca gtataagcac ttcctccaat ttttacagaa gttatgttat
      121 tgttttctct agttatattt acatatacct ttccaggtct gtttataaag ttaccttgct
      181 caccaataaa actagtttta tctaaaatat tgtgatgtac tatataagat gatacgcaag
      241 cgttcccagt tccacataca gggtcttctg taactccaac atatggtgct atagatctta
      301 aatggtagtc acacttttta tctatagttt ctatacaaaa tggagttata ccaattatat
      361 tattatcacc actaaatttt ttgattttat ttaaatctgg agagatattt tttaaatcat
      421 ctaaagattt tagaccaaat acaagccacc aaagtccagt atttaccttt ataataggat
      481 attcaagaag tctatcttca tgcacaccta tcatttgtgc aagttctttt ctgcaattat
      541 ctaaattttc aaattcaggt gtattctgtt ccatcataaa tatattttca ttatttattt
      601 tcttaagctc tacagataat acaccagctt ttgtttcttg ataaaatata cttttattag
      661 tattaagctt cccttcttcc aaaagtgcat ggaaagtacc tattgttgca tgaccacata
      721 aatcaacttc taaacctggt gtgaaaaatt gtactttaaa atctgcaata tttgactctg
      781 aaacaaatgc agtttctgat aaattcattt ctttagccac attctgctta atttcatcag
      841 aaatgttaga ggtatcagta acgacaccag caggatttcc accaaaaggt atagttgtaa
      901 aagcatcaac ttgtttaatc ttaattttgt tcatttttat aacctccttt tatttgttct
      961 taaatataaa tttcatgttt tatgtattga actccaatat ataaaatgtt agagtttttt
     1021 gtccaaattg aaatggcctc actgccttct gataagtttc ctgtcaatat ttcatcatta
     1081 tctacaatca aaataattcc tttgtaaagc attttatttt caagtgtagt tttattacct
     1141 ccatgaatat gaatattatc aagacctttc agacttttgt tttctaaaag tagtatttca
     1201 atattaatat tttttttgta agcaattagg agttcttttt ttaattcaag aatttcttca
     1261 taagggcaag aaattataat ttcttgtttt gcatttttta tcatttctat tgcttttttt
     1321 aatatatttt tatctccttt tatgtttaat acgtattcta cacttttact tttatttaat
     1381 atctgtaagc tattctttag attatccata gaattgtcaa attctttttt cttatttttt
     1441 aataattcat tagggtttat tggtatgtac ttaatagggt cagttccaac attgataatt
     1501 atattattgt ttaatagctt tgttatattt tcatagactt ttgattgtgg tacacctgat
     1561 aatttactta tttcatatcc tgttaaaggg ctttccttta ataatgatat gtatatttga
     1621 gattcgtatt tagtaaatcc tatattttgt aattcattaa taatattatc catatagaaa
     1681 ctcctttgat aaaagattag taactaccta tgtagttatt aatttgattt tataatttaa
     1741 gggaaaaaat gtcaactata ttaagaaaaa aataaaatat attagaaata tttagtctta
     1801 taaatattta atcttataaa tctatttatc ataatattaa atctattaaa aataggcggt
     1861 tacaaaaaag tgcctaattg aactgttata taggtcgtag tatattaata tcaagaagat
     1921 aataaaggag atgtatatta tggtaaaaat agtaaataat aatgaattta taaatgaagt
     1981 agaaaataaa gatggtttag tagttgtaga tttctttgcg acttggtgtg gaccttgtaa
     2041 gatgctatca ccaatatatg aagctttagg aaatgagatg gttgaaaaag caaacttctt
     2101 gaaggtggac atagaccaaa gtatagaatt agctcagaaa tttgaagtat ctacagtacc
     2161 aactatgcta atatttaagg atggaaaacc tgtagataga ttaataggtt ttatgcctaa
     2221 ggagaattta aaaaacaaaa ttgaatcata tatgtaatta aagtaaattg aagtaattaa
     2281 aataaatctc atatataaac aaaaaaactc attgtaatga gttttttgtt tgtatagtgt
     2341 aaaatagttt gtatagttac aaatgagtgt ctaattgcta atgaaattga ctgttgatat
     2401 aatttgcttt tttgtgaaaa caaaactact ctctatttat tgataaaaaa gaatagaaaa
     2461 aacttattta aaagccaaag ttttaatagc atgtataaag ctatccacct catcaaaagt
     2521 attgtacatt cctaagctta tcctaacaag acctacagga ttttccctca taaatctgac
     2581 atatgcttct tttggactta tacctaataa tttttgtaca taaatatctg cacctactac
     2641 tccagctcca gtttctattc caaagttatc aactaaatat tttgctacat ctttgtaata
     2701 catattactt atattaaaag atatatttgg gataattaca ttatttgatt tatctccata
     2761 tattattata ttaggtattt tactcatttc ttctttagca tataaaaata attctctttc
     2821 gtattttgaa attctgttta gaccaatttt atttaaagtg ttcagtgctt ttcccattgc
     2881 aagagttcca aaatactctg ggtatcctgc ttcaaaacgt tggggtacgt ttgcatatag
     2941 tattttgtta tcatttacaa atttggtaga acctgagcca tatagtacag gattaaattt
     3001 ttcaaagaat tttttaggtc ctaccagagc tcctccattt agtggagaat aacatttatg
     3061 agcactaaaa acaacaaaat ctatatgctc atcacattca tgtggcatca ttgaaaaagg
     3121 tttatgctga attacttgca caatatccac caatatcttt gcaccatact tatgagctat
     3181 tcttgctatt tcatatatag gagtggttat acctgtgaca ttactagctc ctgtaactgt
     3241 aacgagagat acattaccag cataattttt aagtatattc tctaattgac ataaatcaag
     3301 ttctcctttt tctgtaattc caacaacttt tgtaggaaaa ttaactttaa aaggcaggta
     3361 attagccata tgctccattg ctgtggtaat aacaaattta tctggatttt catctttaaa
     3421 aaattttgct aagaagttta ttccttctgt agcatttttg acataaataa cagaatcctt
     3481 ttcttcgtct ccatcaataa gtttgaggat agtagagcgt acattttcat attttaaagt
     3541 atttttttct gagattattc ctggagcatt aatataagta agcaaaggaa tctcacagtt
     3601 taagttatcc actacgtttt ttaaaactaa tggagtggca gagttgttaa aatatattct
     3661 tttaacagtt tcaccacttt tagttttaac tttaaggttt gcaccttcaa tgtatttaga
     3721 tacatccatg aattgcataa aaaaacccct tatctaataa actattctgt tttttattaa
     3781 aaaagctcaa gttttaatca agagcttgtt taatatatgt tatgttttta taaggggttt
     3841 tgttaattaa ttcttgttga gatgtttatt aaattacacg aaaatgagct acagttgggg
     3901 gaaactgtag ctcacttgat tttgggggag ataggttgaa cttattgttt atccgtatca
     3961 tatttttagt atagtcactt attttaattt atatacattt ttttataaat tttacataaa
     4021 atgttaaaca ctaattagaa ataattatga atatgactaa taaagttgta aaaatagtaa
     4081 agaaattgat tagaacataa tggaaaatat attgtatata attagaaaaa aagattttat
     4141 atgttataaa agatgtgcaa gtataataat tttataaaaa atacaaaata taatacaaat
     4201 aattttataa aaaaaattat aaaaaaattt aatttatata aattaaaaat tatattacca
     4261 atgtatatat ttgagaaaag attagtacaa agctataatc tgaatttcaa taaatatata
     4321 tataattaag ttgaaacact tgaaaatact ggaaaaaatt gaaaatagtt tttgtaatat
     4381 aaaatgtcga ttgattttaa ataattaaat atacataaga aataattaaa tatatttcaa
     4441 tgaaaatatg ataaaatatg acaaattaat tgatgacaat aaaaaatatt ttgttattgt
     4501 agtattatag ggtaaattgc aaaatatata cgaaagtaaa attttaaata ttgacattaa
     4561 attaaatata ctatactatg taagtgtgat gagagaattt ttttagaggg gatttatagt
     4621 gaatataaaa aagatattta ataacaatgt agtagtaagc tccctagaag atggcacaga
     4681 aataattgtc actggtgctg gagtaggatt taagaaaaaa gtaggcgatt tgatagatga
     4741 aaatttgatt tctaagaaat attttgttca agacgatcaa agggacaaat acaatcaaat
     4801 acttaataag acctctattg aatactttaa aatttcagaa gaaattatag aaaaggctaa
     4861 tgaagtttta aatactcaag tgaatgattc aataatatta gccttaacta gtcacataga
     4921 atttgctgtc caaagagaaa aacaaggtat aaaattacca aacttaatat tgaatgagac
     4981 aaaacaactt tatagggaag aatttgaatt tggattatgg gcaattgatg aaattgaaaa
     5041 aaggatagga ataaaattgc ctgaagatga agcaggatat attgctattc acataatcaa
     5101 tggtttggaa aattctcaaa aagatgaagg tattaatata ctagagtttt caaaacaagt
     5161 tattcagata attgaagaag tacatggatt taaattggat gtaaattcat taaattatac
     5221 tagacttata actcacttaa agttttttgt tcaaaggatt ctccgaaaag aaacttacaa
     5281 tgatagtgat attgaagata tgtataagtt agtgaataaa aattacaata aacctaaagt
     5341 gtgtactgaa gaaattgcag cattggtatt agacagattt gaatataaga ttagtaaaga
     5401 agaagaatta tatttaatga ttcacataaa taaaataaca aacagtggtt gttaggattg
     5461 taactgataa agcaggcaaa acctaagtca ttgagcttaa ctattagatt aagttttttg
     5521 atttaggttt ttttattaga tacaatcaaa atacagacag tgacttttag gattgtaact
     5581 ggttaagcag gcaaaaccta aatcattgaa tttaactatt aggttaagtt ttttgattta
     5641 ggttttttta ttagatacaa tcaaatacag acagtggttg ttaggattgt aactggttga
     5701 gcaggcaaaa cctaaatcat taaacttaat tattaaatta agttttttga tttaggtttt
     5761 tctataaatt tgattatgtt tttagataaa aataggaggg gaaaattatg aaaaaaaata
     5821 agttaggttt atggaacttc ttccaaatgt taggaaaaac gtttatgttc ccaatagcac
     5881 tacttagtgt ttctgggatg atgctaggac ttggggcagg ttttacagac cctaagatga
     5941 ttgaaatggt tccattttta gggaatgaat atgttaactt agttttaaac ttcatgttaa
     6001 caataggatt atttgcattt aacaacttgg gagcattatt tgcaatggca attccactag
     6061 gattattaaa aaaagaaaaa gagtttggtg ctttttcagg attagtagga tttatagcaa
     6121 tgcatatagg tacaaatttt tatttaacaa gagctgattt gttagtacca gcagaacaga
     6181 tgtcaactaa tggtcaagct atgattatgg gtatacaaac atataataca agtgttttag
     6241 gtggtattgt agcaggatta attgtttatg caatatatga taaagttagt aatttaaaga
     6301 taccagaatc attaggattc tacagtggac caagattagt tccaattgtt agtttaatag
     6361 ttatgagtat agttggatta ttaataccta ttgtttggcc accatttttc aatgcattcc
     6421 aaagtttagg aagatggatt tcttcagcag gaccattagg atatttttca tatgcagtgg
     6481 cagaaagagt tacaattcca tttggactaa atcatcttgt aacatctgta ttccgtttta
     6541 caccaatagg tggaactgct gttattgatg gagaaacgta ctttggtaca ttgaacatgt
     6601 ttatggcata tgtagaaaat aatcagatta tacctcttga tttagctggt aaaatggaac
     6661 aaggtaaatt gatgattcaa tatggattag ctggtgctgc attggctatc taccgtacag
     6721 caaaaccaga aaatagaaag gcacttaaag gacttttaat atcaggagtg ttgacagtta
     6781 ttataggtgg tataagtgaa ccaatagagt ttctattctt attcatatca ccagcattat
     6841 ttgctttcca tacatttatg aatggtctag caaacatggt acttccatac ttaggcgtgt
     6901 tgatgggatt cacaggagat ttaattgcat ttataagttt tggtgtgtta agaggaacag
     6961 ctacaggatg gccaatagca gtattagtag cagttttata ttttgcaata tactattttg
     7021 tatttaagtg ggcaattatt aagtttaata taaagacccc aggacgtgag gataaagaaa
     7081 taaaagtatc tgaaggaaat ggttcaaatt tcaaaaattt gtctacatat aaaggacaac
     7141 aaatgatatc tgctttaggt ggtcaatcta atatattatc actagataat tgtgtaacta
     7201 gattacgttt aaaattacaa gatgtatcat taataaatga ggatgcaatt aaagaggctg
     7261 gtggtatagc agttgttaaa ttggatgagc atacaataca agtaattata ggaacccaag
     7321 tgtattcttt aagaaagcag atggataaag ctatggaagg tcatttggag gcatgtaatg
     7381 attaattttg ataaaaaagt agaccgattg ggtacatatt gtacccagtg ggactatgta
     7441 gaagatagat ttggtaagaa ggatttgctt ccttttacaa tttcagatat ggatttagag
     7501 tttccatttg aaataacaga ggtgttgcag gaaaggttaa atcatagagt tttgggatac
     7561 agtagatgga aacatgatga ttttagaaat tcagttacga attggtatat taaaagattt
     7621 aattgtagcg ttgatagtaa ctggatatat tatagtccaa gtgtaatgta ttcaattagc
     7681 aaacttattg agatgttaag taatgaagga gatggaatcc ttataaatac tcctgcatac
     7741 aatgcttttt atgatgtgat aataaataac aatcgtaata ttataaagtc acatcttgta
     7801 aataggaatg gattctattc aattgatttt aatgattttg agaaaaagtg taaaaaatct
     7861 aagatattta ttttatgtaa tcctcacaat ccaactggta gagtatggag tgagttagaa
     7921 cttgttaaga tgataggaat ttgtaagaat aatgatgtca agataatatc agatgatata
     7981 cacatggata ttgtatatga caatagaatg acacctatct taaatgttgc aaaggactat
     8041 ttagattctg tattcatttg tacatcagcg tctaaatctt tcaatatacc agcattgact
     8101 gggtcatatg tgattatacc aaatgaaggt attagaaata aatttgaaaa tatcactaga
     8161 taccgtgatt ttgtaaattc gccagcaatt ctagcaatat tagccacaat ggtctgttat
     8221 gatgactgcg aatattggtt agatgaacta ttaaaatacc ttaaagagaa tcttaaatat
     8281 acgattgatt atataaaaga taatttagaa ccattgaaat tggtcatgcc agaaggctgt
     8341 tactttgctt ggatagattt ttccgaactt aaaataagtg gtgagaaatt ccaaaaatta
     8401 ttgatggata ttggagaagt tgctattatg tcggggtctg tatatggtga agaaggtgag
     8461 ttttatttaa ggttaaatgt ggcatgttca agagaaaaac ttgaagatgg tttaaatagg
     8521 attaaaaaaa ctatggatta tattgaaaaa agaattttat agtaggaagg gagatagtta
     8581 tgggatattt aaaaacaatg gttgatgtta tgactactga aatgaatgcc ataagatgtt
     8641 taatagatga agcaggaata gaatatgaaa gtatagttag tgaaattgca aattgtgaag
     8701 gcaaagtaat ttttatgggt gtaggaaaat ctgcacatat aggtaagaaa ttggcagcta
     8761 catttgctag tactggtaca cctagctttt ttgtacatgc aacagaggca gtgcatggag
     8821 atttaggaat gattgaaagt aaggatatta caattttaat atcaaatagt ggaaattcta
     8881 tggaagtggt aaactgtata aaatatatta aagcaatagg ttctaagaca attgcattta
     8941 cttcaaacag aaattcagta ctggcaaaag agtgtgatta tgctttaata tatcctgcaa
     9001 aggatgaagc agaccattta aatctagcac caacaacatc atctacaatt actttggttt
     9061 taggagattc tattgcatgt gcattatcta agagtagtaa ttttggttct tcagattttt
     9121 ataagtacca tccaggagga agtttaggtg agaagttaaa aacagctaac aatggataaa
     9181 agcaaaaagg agagtagttt ttatgttcaa aatgtttaaa aaaaaatcaa taaaatcacc
     9241 catatcagga aaagtaatag agttatctaa aatacaagat ggtgtatttt ctcaaaaaat
     9301 tatgggagag ggtgtagcta tagattctac aggagacatt gtatatgctc caatcaatgg
     9361 aaagatagta gtagtagcag agactaaaca tgcatttgta atagaattag aaaatggaat
     9421 ggaacttctt attcatgttg gtttagatac tgtaaattta aaaggcgaag gttttgaggc
     9481 attggtgagt atgggagaac aagtgaagga aggaacacca atgttgaaaa ttaaccgtcc
     9541 tttgattgaa tcaaagggta tatcacttat tactccagtt acaataacta atcattcaga
     9601 gtacaatatg aatacatgta atgtaggcaa tagtgttgaa ggtgggaaag atactgtaat
     9661 tgagtttaaa taaagaacta aaatacaagg ttaatattta taaataccta taaacttatg
     9721 ttttataaga ttgattgtat agatttgttg tataaaaaag aactctttaa ttataaatta
     9781 aggagttctt ttttattttg taaatattgt ttagatttat atagaaaaac acttagtaat
     9841 aaatagaata aataagtatt tatatagcta aaaatctaaa tagttttgtt taaatatatt
     9901 tttatatttt gtaatttcaa attaataaat aattagattt gataaacaaa aaataaaaat
     9961 gttgattata ttaataaact ttaatataat atatttatga aaaatgtaaa ctgtgaaatg
    10021 gaggtagtaa catgaaaatt gaaaatcttt ctattgatga tattaaacga ggctatatat
    10081 ttaatgatga tttaaattgt tatagttgca ttaaatgtac aaaaacatat gaggtaggag
    10141 aaatatataa aataggtaat agattctttg aagcttcaaa agctattgaa cttcatatgg
    10201 aattagaaca tagtgattat ttagagcaat tgcttaatag tgattcaaaa tacaacacat
    10261 tgactgaaaa tcaaaaacaa ttatttcaat tatttattag tgatttacca gataaggaga
    10321 tagcaaaaca ggttggagta tccacatcta ctatacgcca tcaaaaattt attttcagag
    10381 aaaaagcaaa acaggctaag ctctatttag cactatatga atctgtattt gaagataaat
    10441 ctaataaaag tagtactata ataccaatcc atgaacatgc tcgaatggta gatgaaagat
    10501 atgtagtaac tgaagaagag agaaaacaca ttttggatac atcatttgaa agtatgaatc
    10561 ctttaaaatt aaaagtattt tcttcaaaag aaaagaaaaa ggtcgtaatt ttatctaaga
    10621 ttttggaaca atttgaacgt gggaagaaat attcagaaaa ggaagttaat cagattttag
    10681 aatctatata tgatgatttt gcaacaatta gaagatatct aattgagtat ggttttatga
    10741 gtagaaataa agaatgtaca aaatattggt taacataatt aaaataataa attttactaa
    10801 tgtgtaatgt tatttttatc tagggggagg agtcaaatat gaatgatgaa ttaaaatcta
    10861 ttttatacaa taaaggtgtg gacattgttc gctttgttga tatatctgaa tttccaataa
    10921 atcaaacgca aggtttttct aaagcaattt tattttgtat agggttatca aaaaaattta
    10981 ttacagatat atacaataac ttacctacag atagtgatga gtttttagaa aaagaggaga
    11041 aagcagaaga attagctgac tggatttcag aatatattca aaataaagga tatagagcgt
    11101 attctcaatc ggagaaaaac aatctagaac atggatattt tgaaaaagcc tatattaatc
    11161 cagaaatgca atcagggatt agtcctttac cacataagac aatagcaaat attagtggca
    11221 ttggatttat gggaaaaaat aatttgtttg taacagagga atacggatgt gcttttagca
    11281 tgtgtacagt acttacagat gctccaattt ctgttgaaag atatcctttg attgattcaa
    11341 aatgtatgga ttgtaatgtt tgtgtagaaa actgccctgc aaaagctata catggaaatg
    11401 agtggacttt gccaggaaaa agagagtcta ttattgatgt ttcaaagtgt ttttgtgtat
    11461 tgaagtgtat gatgagttgc ccatggtcat taagatatgc aaatcaaaaa taattttact
    11521 agagaaggta ttaaattgaa tattataaaa tttttaaaat gtaagattat ataagatttt
    11581 tcaaaataaa aatttgtata gaacactcag aaatgagtgt ttttttattg gaattaaatt
    11641 atattgatac aaagtttaac tattttaatc attatataaa atttgtatta aaaaattaag
    11701 aataacttgg taatagtgaa aatataatga gattataatt gatttataat ataactataa
    11761 tgatgttgta aaatgaaaat tgtcatttca aaattcccaa aaagagtaaa agtcacttcg
    11821 gttatagtca aaaaattttt tcagtgaaaa gaatttagat ttaaaaggga ggaattaatc
    11881 atggaaattg aaactaaaac tccggattgg attaatgtac tagaattaaa cagtaaaata
    11941 gatattactg ggaaatatga tgtaagtaat attatacaag atattgtctc tgataagtca
    12001 ctagaaggtt ccattatata ttttccaaaa ggtaattatt tatttgagaa aggtattaaa
    12061 attagtcaac aaataacact tcaaggagat tcttattatg gaggagataa tcaagtatca
    12121 aatttagata aaaaacaatc aatcatagga acaaccaatt ttataactcg aggagtttct
    12181 aatatgagta ttattgcatt gacaggaaca agtcaatgta tcaaaaatat taatttttat
    12241 tatgatagtc atgatataga gaaaataccg cctaaaaatg tatctgctat tacagaatat
    12301 ggagaaacac aaggactatc tcattttgaa cacctgttta tttctggatt ttcagggata
    12361 gggattgaaa ttccatacta ttcaactggc aatgatataa ttgttagttc atgtggtctt
    12421 ggtatgaggc ttggagagaa gtcaatgctt tctagtagca agatttacga atgtaaaaat
    12481 ggaatggaga ttactactgg tgttagctta aataatgtga gaatagaaga aataaaagaa
    12541 ataggtatta ataataaagg atttggtttt aacctgatta tgaatcttat ggtcgaccaa
    12601 tgtggatatt gtggatttat gtttgaaaaa atgtgctata gccagataac tgccagaatt
    12661 actagatgcg gtcagtatta taaaagtgtt gattataatg cttatgagaa tatggaagat
    12721 agaaaagaaa aagcttattc cattttttat ggagatattt tagaaagttg caatattgta
    12781 ttgatgaata gaaatattga taccttagaa agtgattcac ttaatgaaca taagatatat
    12841 gtaatcaagg caaatgaaac aaacaatgta atgctaacat gttatgctga agcagataac
    12901 tttatacaat gtgcaaaggg caacttgctg ttagaaaatg gtgaaaatac ttataaattt
    12961 tataatggtg agatttgttc tgtaggaggt gttcaaattt ctgatataga tgaaaaagat
    13021 ttaatcaaaa taatagagaa cactttgtat atagatgata aaggtaagta tttaatggtt
    13081 cctagattaa aaaagaatac tgtaatatat tcagtactag attctacaga agaaataaat
    13141 aaattacatg gtggaatatg ggaacagatt gatgtaaaag tattagctgg ggaatccatg
    13201 tactattata aaaagataaa agattaaatt atcaaactag gaggtagtga ttaatggaag
    13261 agtctataat aattaaagat ataagtggct ggattaatgt acttgattat ggaatttgta
    13321 atgatgaaac aaaagatgta agttttaagt tgcaagagat tatggatgct gtaccagaag
    13381 attctattat atattttcca aaaggaaaat acttatttga aagtggtgtt aagattaaga
    13441 aacgattaac cttatgtgga gattcttata ttttagcgaa tgttcctaaa aatactgctg
    13501 gagtaacaca gtttactttt aaaggaaaga aaaatcctaa aagtgaaaat agagcaatca
    13561 taacagcaaa aagtgtaaga cactgtatta agaacatttg cttttattca gatagttgtg
    13621 atagagatat gacatctaat catgaacctc caacagatgg aaaacctaga tttcatcata
    13681 atgtaattat aaattataga aatatatctg ctgttatatg tgaagataaa cagggagaac
    13741 caggtcatta tgaaaatcta tcttttagtg gtttttctgg aattgcactt gatatgccta
    13801 ataattctac agcaaatgat attacaattt ttacatccgg acttggaatt agtacagggg
    13861 aaaattcaac aattgctaac agtaaggttt ggggctgtgt tgatggaatg ataatttcta
    13921 ctggaacttt tataaatggt atgagggtag aagaaataaa aaaagttgga attaaaaata
    13981 ttggtagagg tttaaatttt attacaaata tcacaattga ccaatgtgga tattgtggag
    14041 tttggtttga ttctatatcc aatgttcaga tatcaggaaa tatcacaagg tgtgggcagt
    14101 actattatga tgtaagctat gataattatt taatgttgga taatagagtt aaagaagcat
    14161 attctttatt ctatggtaat ttattggagt caagtaatat agttttatca aataccaatg
    14221 aagataattg ggaagatttc aaagaagata agcataaggt gtatcttata gaagcactta
    14281 aaaccaagaa tgtactatta acatgcaata tagatgctac tgactggatt gttaagtcag
    14341 aaaaaggaaa tttaacgtac aataatatgc gacaaactgt tatattttac gatggaaaac
    14401 taagtagtat caatggaatt ggtatatctg atgaagatta tggggataaa gtcaagataa
    14461 aaaaaggaaa tatatatata aatagtaatg ataaggatat taagatgcat aaaccagagg
    14521 taggtgacat tatgtcaaca attattaaag ataaaagtat tctttctgct cattatgggg
    14581 gaacatggga gaaaattggt gaaaaaatag agtttggaat aactgtacac tattataaat
    14641 tgatagagaa ataagtaaat acgtagtatt tgaatagatg tattttattg gagagttagg
    14701 actatagttt taatataaat ggtttggtaa tagcatggat tgaataatat aaaaataata
    14761 taaaaaatta atttgattat gtaaatttct atgaagtagt aaaactgtga ctttagtcat
    14821 aggaggttca aactaagtaa aagggaaagg aatatataat attatggaag gaagaactgg
    14881 gattaaagat ggaagatgga ttaatgtagt agaaagaggt attaaaaatg atggttcgta
    14941 caacataggt cctgctttgc aggatattat tgatgaaata gaaacagatt tttgtgtctt
    15001 atattttcca aaaggtaaat acctatttac aagtggtgtt aaaattaata aagaaattgt
    15061 attgcaaggt gattctaatt catctacagg tactacacaa tttataactc gtggagtaga
    15121 aaatatgagt attattacat tgacagggaa gaaacaatgt attaaaaata taaatttcta
    15181 ttctgatagt tgtgagatac aggtaaataa agaaccaccg acaaaaggta atcctaaata
    15241 tcattatgag atgattatta ataagaatga aaagaatgtt gagatgaata atgtatcagc
    15301 aatcctgtat gataacagta agaatatgat taaaggtcta ggtcattatg aaaatattta
    15361 tataagtggt ttttcgggta caggaattag gataccatat tactcaattg taaatgatat
    15421 tacagtttca tcttgtgggc tgggtattga tacaggaata gacacaatta tttctaatag
    15481 tagaatatct aagtgtcaaa atggaatgag gattagaact ggaactagta taaataatgt
    15541 aaggattgaa aaaatacaga aagttggtct tataagtagt caagttaagg ggtatgaggg
    15601 gtatggaagt tataaaatta ataatattac gattgaccaa tgtgggtatt gtggcttttc
    15661 atttgattca atgaaagata gttatttttc tggtattata agaaggtgtg gtcagtatta
    15721 ttataataca gattatgaca catatttaag tattaaagat agagtagaag aagcatattc
    15781 tttattttat ggaaattatt ttacagaatg taatattgat ttagttagtg ataataagga
    15841 taactgggat gatggcttag aatataaaca taaagtttat gtatttaaaa cccttcaaat
    15901 accaaaatta acattgagat gcaatataga tgcctatgat tatatcgtta aatcggtaca
    15961 tggaggaggt aatttaatgt taaaaaataa ctttaacaat tatatattta ttgattcatc
    16021 taatccagaa gttattaatg gaattaattt taattatcaa aagaatggaa acttgttaag
    16081 gttaaataat ggtaaattaa gttataaaaa tggaaaggaa gaaacagcta tattcagacc
    16141 taattatggt cttattgtag catcaacaga ttcagaatgt gaacaaatag aggctaccta
    16201 tggagaaaaa tgggagaagc ttggttctga agtaattgga gaagaaacgg tatattatta
    16261 taaacatcat gaaccagata aaacaaacca aatacaataa aatttgtatt atataatgat
    16321 ataaagtttt aatatatagt gtttaccttt aatgtaatat tggtatattt gtatagtatt
    16381 aaatttgtta aaaagtaaaa ttgtagggtg cttgaaaata gcaccttttt tagtttcatt
    16441 ataaaattag ttttgaaagt ttaggaataa tgtatattta tttttaaaga attataacct
    16501 tattaaggat attttaaatg ctagaaatga atcagtaaat catactggaa ttatcttaaa
    16561 tgttttgatt aaattaagta tttagagcta taattatatt aaaaatatat tagaacataa
    16621 ttatattact atggaggtta aatatgatag taagtgtgag tagaagaact gatatacctg
    16681 ctttctatag taagtggttt tttaacagac ttgaagaagg ttttgtttat gtaattaatc
    16741 caatgaatcc aaaacaggtt agtaagatag agttaaatcc acatactgtt gattgttttg
    16801 tattttggac aaaggatgta actccaatgt tatatgattt aaataaatta agagactata
    16861 aatattattt tcactatact ataacatcat atggaaaaga agtagagaca tgtattttag
    16921 ataaaagaaa ggtaatagat tcatttaagg aattgtctag aaaaataggc aaagaaagag
    16981 taattttaag atacgacccg attttcttaa gtgaaaagta cacagtagat tatcatatca
    17041 aagcatttgc gagtttatgt aatcagcttg atggatttac agaaaaatgt ataataagtt
    17101 ttatcgattt gtataagaaa acaaaattta atactaaagc attacatata aagccaatag
    17161 aaattacaga aatagaaaca ttatcaaaag agatttctaa gattgctcat aagcatggga
    17221 taatacttga aatgtgttct gaagaatata atttaagtaa atttgggatt ggaaaaagta
    17281 agtgtattga tgatagatta atatcaaaaa ttataggatg tgaagtagag gtaaaaaaag
    17341 atagtacaca aagagatatt tgtggatgtg ttaaaagtgt cgatatagga caatacaata
    17401 cttgcagaca ttattgtctg tattgttatg ctaactttaa ttatgcaaaa gtagaagaaa
    17461 actgtaggct ttataaggaa gataataaat tactcataga tactttaaga gatgatgcaa
    17521 aaataagcat tagagatatg aagactatta aagtagataa gaataaacaa attagtatat
    17581 ttgattaaaa taaatatact aattacctta atatattaag aatatagata cttttgataa
    17641 caaaattaaa cttaatagat attttctata taagaaagat ggtcataata tgtcaatttg
    17701 accatctttt tgatataatt aaaacacaaa taatttagta acaaaggagg cagaacttga
    17761 atttattaaa aaaatatcct atacctattg caagtttgat tttatcaatt tttactttag
    17821 gtaacttatt acaatcttat agtgaatcat tacgtaatgt aattggattt ataggattta
    17881 ttttgtatgc aatttatata gtcaaaataa tattacttag aaagggtgtt aaagagcaat
    17941 tagagaaccc tttaatatca agtacatttc caactataac aatggcaaca atgatatttg
    18001 ctacatatat aaaaccatta tctttagaat taagtattat gatttggagc ataggaattg
    18061 tgggtcatct tgtgttaata gtcttattta gtaagaagtt cttagttaaa ttctcaataa
    18121 aaacggtatt tccatcttgg tatatagtat atgttgggat agttgcagct agtgttacgg
    18181 caccagcatt taatcaactt ttgattggca aaattgcctt ttggatagga tttgtatctt
    18241 atatagttct tataccagta attttaaaga gagtatggat aatcaaggaa atgcctgaac
    18301 catccttacc aagtatagct atattagctg caccagggtc actattattg tctggatata
    18361 ttaattcttt ttcagataaa aataatgtag tattatactt gttattcata ttatcaattg
    18421 tattttatat tatagttttg ggatatttac cgaaattatt aaggttgcct ttttatccga
    18481 gttttgctgc atttacattc cctatggcaa taagtgcaat gggtatgaaa ctaatgaatg
    18541 gttatttaac taaatcaggt cattttataa gttggatttc atatgcttct aagttagaag
    18601 agataattgc tacagtgatt attttatatg ttttagtttt gtttattata tatcttagta
    18661 aatctgaaac tacatctaaa gctaaataag aattattaaa tatgtgtatt taataggaat
    18721 attgatataa atatatgaag ataagataaa ccatagaaac ttaatttagc ttaattttat
    18781 tggaggaaaa caaatgaaaa caaaagaaga atatttaaaa gaagttagaa acatatcaga
    18841 agaatacttt agaaaaggag agtttttttg ctctgaagca gttctccaga caataaatga
    18901 tgctttaggc caacctttaa gtccagagat aactaagtta gcatcaggat ttccgatagg
    18961 acttggaaag gcacaatgtc tttgtggagc aatatctggg ggagagatgg ctttaggtat
    19021 agtatatgga agagttcatg gtgaagctat gaatccaaag atgtttgagc atgctaaagg
    19081 tttgcatgat tatattaaaa aagagtatgg tgcaacatgt tgtagagcta ttacaaagaa
    19141 atgggatggt gataacttta tgtctcccga aagaaaagag cactgtatta aaataacagg
    19201 tcaagttaca gagtgggttg cagataagct tatagaagat gaaaaactta atatcaaata
    19261 gtgtaatatt agttggtgaa tttataaagt taaatttact tatagtataa gatacttgat
    19321 attctcgtat tgatagaaaa atttatagca tatgtattta atgaaatata cttatattta
    19381 gagtgtggtt taatttcata aatacatatg ctattatttt aagtatataa gcaaatttat
    19441 ttaaataaat agaaacagtt aatttatatg tacaaaacta tatatgatta gtgtaaataa
    19501 atacctattt tataatactc aacataaaga caccagacat gattagtatt ataccaataa
    19561 aaatagcaag ccattctttt ttgttcttct tttctttaaa aaatataatc cctcctaaag
    19621 tggctactat cacacaactc tgagagatag aaaaacttat tgtaactccc aatgtattag
    19681 tcgttatgaa catacctaaa tttgctattg accacaatat accagtaatt aaattgaata
    19741 aaacattttc cctatataca atctctttag aaaaatactt gacaataata gatgaagtta
    19801 tcaacatacc aacagattga ggtaatataa ttctatatcc ttcaattaaa aatatttgat
    19861 tagttacaac atataatgat aaaaatagag atgataataa tacaactcca acacatttta
    19921 acatattttt atctgtagat tttttagttt gataatttgt gagaagtata ccaagtatta
    19981 tacataatat tgataaagaa ccaatcagta acattttaga actggtccat tcattaaaca
    20041 agattacagc taataatgtc gtaaaagtta attgtgaacc atttgaaatt ggcattactt
    20101 tagatacact agcaatttga attgctttaa actgcattaa ttgtcccaaa ctccagaata
    20161 tgccagaaat catacttata caaaatgaaa aaaatgtaat ttcaggatgt ataaccagag
    20221 ttattatagt ggaaaatata aacgcactta tggatgtacc taaaagctgt tcatgaggag
    20281 aacttttctt tagatttgca actatgggca tcaatcccca tcccattaca ggaagtaaag
    20341 caataattat tgtcagcatt aaactatttc ctttcatatt aaatttagac agcttttaat
    20401 ttatcatcta aaattaaagt gttcaataca taaaaattac cgtatttaat caggaaatat
    20461 tagtagtttt ttagaaaaac attttccatt aaaataaaga caaagggaag gagtaaaatt
    20521 gaaaatgtta actgatatga ttcaaaatcg tgaaattaaa ataataaagt ttttattaaa
    20581 agaaggtcat acaactgtaa aagcaatagc agatgattta gatgtttcag aaaaaacagt
    20641 ttcaaaatct cttaaagaag tagaagcagt gcttaaaaag ataggtcttt ctttaataag
    20701 aaaacctaag ataggtgttt atattgatgg agatttaaaa aaaatacttc cttacctaga
    20761 taataaagga agtcaaattc ctacaaccag agaggagaga gttatctata tatattcagt
    20821 acttttaaag agtaatgatt atattactat acaacaactt tctgatgaac tatatataag
    20881 tcgtggaact attgaaaggg atttagcaga agttgaaaaa ctgctaaaaa acgaagggat
    20941 ttccatatat aagaaaccta gtaagggaat aaagttaaat ataagtgaaa gagataaaag
    21001 acaacttact gctaagttta tacataaatt ttggagtaga aactggtata ttaagcaaga
    21061 gcaagaaagc ttttatcaag catttgaaaa gatacaagca gatgtaaatg gtattttttc
    21121 ggaagatgat ttatcaataa ttattgatat tcttaggaat ttcagcagag aaagaaaatt
    21181 tgagttctct gattatgaat ttcagtcatt agttattcat ttggcaatag ctgtagagag
    21241 aattagaaaa ggcgaatata tagaagaaag tttgtatact ggtaatatag aaaataatca
    21301 aatgtcaaat accaattatt tagcaaaaca attagaagaa aaattttcaa tattattacc
    21361 aaaatctgaa attggatata taaatttaca tttagtagcg tctgaccaac atagagatac
    21421 taatgaacta aaaaatactt caactaatcc ttgtgagaca agtacaaata atatatcaaa
    21481 tcttcgggaa attataaaaa atacattgta tgagacagct tatgacaagg atttaattga
    21541 tggattaact tcacatgtac attcatcaat aaatagactg aaatatggac ttagtattaa
    21601 aaatccatat gtaaatacaa tcaaacaaaa cttttcttta tcatttgagc aagctttaga
    21661 tttaaagaaa gtaattgaat ttatatatga tattaggata aatgatgatg aatgtgctta
    21721 tatagcatta cattttgaag cttttagaga aagaatgaaa gaacttccac aaaatattag
    21781 agtaattcta gtatgcagta caggattagg ttcatctagg ttattgtctg caagaataaa
    21841 aaaatacttt ccaatgataa agattgaaaa agtactttcc gtacaagaat tagtatccag
    21901 taaaattgat gcagatttag taataagtac tatttattta gagttagaaa caattccaac
    21961 catagtagtt agtcctattt taaatgaaag tgatataaga accttggaaa gaaatataca
    22021 aaatgtttca agttatactt ctaagaagta taaatctttc agaaacttaa tctttgaaga
    22081 aaatatattg ttgaatattg aagcttcaaa ctatgaagat gcaattaagt atataacaga
    22141 taacctaata aaaaaaggtt ttgcaaaaga aggtatagct aaaagtgcaa ttgagagaga
    22201 aaatttatct tacacttctt tccaatcaat agctacacct catgctaatc ctagttatat
    22261 taaaaaatct aatatatctg ttgcaacgtt aaaaaatcct attacatggg gaaatgaaaa
    22321 ggtggaagtt attttcttta tttcattaga aaatgacaaa aacctagagc ttgatgaaat
    22381 atatgaagtt ttttatgact tcattgacaa taaaaaaaat ataaatgaca tattaagtgc
    22441 aacttccaca aaggaagtgt acgatttgct agtggaggag agtatatagt gaaaatatca
    22501 gatgtattga aaaaagagca aattgttttt aatcttgata caaatacaaa ggaagaaaca
    22561 ataaaaaaat taagtagtat atttgttgaa aatgggattg tggacaatga agaagaatat
    22621 gttaagtctg ttttggaaag agaagaacat tcaactacag gtattggtaa tggtattgct
    22681 attcctcatg ggaaaagtaa tgctgtcaaa aaatcttcta taatatttgc aaagactaag
    22741 cataagattg aatgggatgc tttagatgaa aaaccagtag atagtatttt cttattggct
    22801 ataagcaata ttgataagga tagcaatcat cttgtactat tatctaaatt agcaactaaa
    22861 cttatggatg atgacaatgt agatgcacta aaattagcca attcagaaca agaaataatc
    22921 aatatattta gtgaagaagg agagatgtag aaatgaaaag aaaaattata gcagttacag
    22981 catgtgcaac aggagttgca catacatata tggcagctca agcgttaaag aaagcttcaa
    23041 aaaagatggg tcaccttata aaagttgaaa cacaaggagc tacaggaatc gataatgagt
    23101 taactacaaa ggatgcacaa attgcagaag tagtaatatt tgcagtagat actaaagtca
    23161 gaaatgctga acgttttgag ggaaaagaaa tactagaagt accagttaat gcacctataa
    23221 aggatgcaga gaaagtaata aatgatgcac taaagttaat tgacactaaa taaatggagg
    23281 gaaatgtaat gttgaaaaag atagtctctg agttaaaaaa gcatgtctta actggaatct
    23341 catatatgat tccattagta attgcaggtg ctatgataat ggcgatatct cgtgtaggtg
    23401 gttctttcta taatatacca gatatatggg atgctaaata tgcagaaagt gctagtagta
    23461 ttgttagatt attacatgat ttagatgggt ttggaggtac tgcattagga ttaatgtttc
    23521 ctgtaattgc tgcatttata ggattttcta tagctgataa attagctatt gtaccaggtc
    23581 ttgttggagg tatgattgct aaagatatag gtgcaggatt tttaggagct ttggcagttg
    23641 gtcttatagc aggatataca tgtttgttta ttaaaaatca tgttaaatta ccaaaatcag
    23701 cagcttcaat agtaccaata tttatagtac cagtatttgg gacattaatc actgttgtat
    23761 taatcaatta tgtgataggt ataccttttg ccacattaaa tactggatta gaaaattggt
    23821 taaatggatt atctggtaca aatcagatac taatggcagc tattataggg gctatgatgg
    23881 cagttgactt aggtggacca gttaataaag cagctttaac aacatcactt gcattattaa
    23941 caagtggaat ttatgcgcct aacactgctg caatggtagg aatagttata ccaccactag
    24001 gattgggact tgctactata ctagcaaaac aaaaatataa taaacagtta cgtgaagctg
    24061 gaaaatcatc tcttataatg ggtttaattg gagtaagtga aggagcaatt ccttttgcgg
    24121 tagaatctcc attaaaagta ataccttcat gtgtaattgg aacagcaatt gcttcagcta
    24181 tggcagttgg tttaggttca gtaaatgcaa ctccaattag tggattttat ggatggttta
    24241 cagtagagaa ttggccaatg tatgtgttat caatagcagt tggaacagta attgtagctg
    24301 gattagttat agtattgcgt ggaaatccag tgattgagga tgatgaattt gaagatgatg
    24361 attttgatga agcagagtgg gaatcttaaa atttaataac tatttaagac gtgttataag
    24421 ctatattcaa tatgaatctt atactaaata aagtgttaaa cattcagttt agacttaaat
    24481 ataaaagata aagaacagag gagaataaat gataaaagca catattgtta atcatacaca
    24541 ctgggataga gaatggtatt ttacttctgg agatgcatta gtacttagtg aacaactatt
    24601 tacagatgta attgatgaat tagaacgtaa tccagatgta agttttgtac ttgatggaca
    24661 actttctatt ttggatgatt atgtacaatt acacaaagaa aaaatagaaa tcattaaaaa
    24721 gttaataaaa gaagatagac tacatattgg accatggttt actcaaactg atgcattctt
    24781 tgctagagga gagtctattt taagaaactt aatgattgga atttttgaaa gtaaaaagta
    24841 tggtaagtat atgaaaattg ggtatttgcc agatacattt ggatttaatg cacagatgcc
    24901 tatactatta aagcatgtag gtcttgataa tatattcttt tggagaggta tacatcttgg
    24961 taaacaggtt caatcaccat attttaagtg gaaaagctta aatggagaaa gttatatata
    25021 tgcagtaaat atgccacatg gatatggaac tggtatgctt ttagagcctt cattaaagta
    25081 tgttaatgga cgcttagacc cagctattga ttttattaaa tcatatactg atgtagatga
    25141 ggtgttaata ccttctggga atgaccagtt aaatataata ggtgatttca aaaataaaat
    25201 taaagcgatt aatgatatcg gaaagtattc atatataacg agttcatatc aagagttttt
    25261 aaaatatata aagtccatag aatcactaga aagctataga ggagaattta gagaacctgt
    25321 attagcaaga atacataaat caattggttc tagtagaatg gatataaaat tagcatgtga
    25381 taaactagaa aacaagctga ttaaaagaat agagccatta ttagtaattg ctaaaaagag
    25441 taaaattgaa ataagcaatc agttacttat aaatacatgg aaaaaacttt tggaaggaca
    25501 agcacacgat agtttagctg gatgtgtaac tgatactgta acagatgata ttcttcaccg
    25561 tataagagaa gcaaatgaaa tatgtgacag tattgaaaat actattgtaa aaaagatttc
    25621 agaagggctt aaattatcta aaaatgatat tttagtattt aatacagaag ctaagcgttt
    25681 taatggatat aaagagatac aagttgtaag tgatagtaaa aatatttatt ttaaagataa
    25741 tttagatgct actataattg aagaaactta tgtaaaaccg agagaaaatg tattagaaga
    25801 aacacctgct ggtaatatat ttattgaaga accaggatat tatatattaa aagtaagaat
    25861 aaatgtacag cttcctgcac ttggttataa agttgttagt tttgaatcaa gtgataaaga
    25921 aatggtgtca cttaataaat ctaatgatgc ttttatatct aatgataatt ataagataat
    25981 atatgaagat ggaagcttaa atttacaact taaagatgga acttacatat caaacttttt
    26041 aacattaaaa gatagtggta atgcaggtga tacctatgac ttttctccac taaaaaatga
    26101 tgaggatata aaactttcgt ttgataaagt gtatacagaa aaagctttag gatatgaaaa
    26161 aatggtggtt aaaggtacta ctttactacc acttacgtta gaagatagaa aaaacaaata
    26221 tcttaatggg aaattaaatg taaaagtaag tattgtctta tccaaagaaa atccattgat
    26281 ggatgtaaaa ctttgtgttg ataatacgat atataatcat agactacgtg ttcatataaa
    26341 aactgatata aaagataata aaaacatagc atcattgcca tttggatata taacaagaga
    26401 aaatggagtt ttagacaact gggaagatat ttacagtgaa atgcctatag atttagaacc
    26461 tttggaaagt aatattactc taacaaacca aaatagaagc tgtactatat ttactaaagg
    26521 aataaaagag tatcaacata ttgaagatga aattgcattg accttacttg ctactacaga
    26581 tgaactagga aagcctgatt tattatatag accaggacgt gcttctggtg atacaactaa
    26641 aaaaggtcac ataagaatta aaacagataa agctcaaatg cttaagaaat tagaattttc
    26701 atttgcaata tatatggatt ctaaatcatt tgatgaattg gaaattgcga atttaaccca
    26761 caattatcta agggaatgtg taaattatca attacaggat tacaatttct ttttatatcg
    26821 tatagataat aagattcaaa aatcaattgt caataaaact gtaagtagag aattagaaat
    26881 aatatcttta ccagaaaact atcttatatc agcatgttat ccttcttatt acagtaaaga
    26941 taaatttata ataagagtgg aaaatccaag tagtaataaa atgatattgg atgagcaaat
    27001 atttaaaaat agaaatggga aaattgtaaa tgcaatagaa gatgtagaag aggaacaggt
    27061 atatgaaata gcaccttttg acgttatttc aatattattg gatttataag tgtaaaagta
    27121 agtttattgt ttaaagtctg acagatatta atctgccaga ctttttaatt tgtataaatt
    27181 tgggtaaata ttaatgaatg gttttagtat ttaagtatga atttagtatt taatatagaa
    27241 tttattgaag tagaataatt agtttaacag gataagcagt ttaaataaag aattattata
    27301 ttagagtgat tttatatata atttaagata ataaaatttt aataaaatga gttaaagatt
    27361 gtatattaat agagttgatg tagttacaaa aaagttacca attgttatgt atttacaaaa
    27421 ctgatatatt aattacataa aatctaaaat agtttgtaat aggagtatat ataataaaaa
    27481 ttttaattaa ataaagctag gtatatttat aatattaata aatatattgc tataaaagtt
    27541 tgtttaataa attaaataaa gaaaagagga gaataaaatg tacaacatag atgatatgat
    27601 atataattgt ccagtagaag ctttatcaaa tattttaggt aaaaaatggg tggctataat
    27661 tatatggaaa atacaagaag ataaaaaacg atttggagag ctacaaagag ctataccaga
    27721 ctgtagtaag aaaatgttag ttcaacaact agatttttta attgaacagg ggattatagt
    27781 caaccaaaaa aagttggtta ataatgttgt agagtcaaca tattttttaa gtgaatcagg
    27841 attacgattg cttcctgtca tggaaaaaat gattatgtgg agcaataaaa atttaaattg
    27901 tgataaatac taaaatttac tctgtaagta atgaaaattt aattaagcta tgagtagaaa
    27961 aatgtattaa aattacataa ttttttagtt agattttaat ttagaaatgg gattgtaata
    28021 ttggaaataa aataaggagg aattttaatt tgcaaaatgt aaatttattt ttagtattta
    28081 tagaaggtat agtatcattc ttttcaccat gtatactgcc aattttgcct atttatttga
    28141 gtatattgtc aaatagtagt gtagaaaatt taaaagaagg aaagacgagt tttataggaa
    28201 gttctttatt taaaaatact atattttttg ctcttggaat ctcaacaaca ttctttatat
    28261 tgggttcatc agtaaaagta ttgagtatgt tttttaatga aaataaagat ttaataatgt
    28321 ttattggtgg gattataatt attatcatgg gattatttta catgggaata attaaatctt
    28381 caatattgaa tagagaaaaa agatttaatg ttaagtttaa agaaatgaaa gcaataacag
    28441 cttttatatt aggttttaca tttagttttg gatggacacc ttgtattggt ccaatattgg
    28501 catctgtatt agtcatggtt tcaagttcaa gtaatcatct tagtgcgaat cttttaatag
    28561 cagtatatac tattggtttt attttgccat tcataattac agctatgttt tacagtaaac
    28621 tatttaaaac tatagataag ataaaatcta acatggaaat tattaaaaaa ataggtggga
    28681 ttatacttat agtatcagga atattaatga tggtaaatgg atttggaagt ataagcaaac
    28741 actttaatac gtcacaaaat agtaaaattg aaagcaagca agaagaaaat aagcgagaaa
    28801 attctactga taaagaagaa aattctgatg gtaatgatag tcaaaaagac tctaataatg
    28861 acaataatga taaagggtct aatgatgaag atagaataaa gtctattgat tttactttaa
    28921 cagaccagta tggtaaaaca cataaattaa gtgattatga aggcaaagtt gtattcttaa
    28981 atttttgggc tacatggtgt cctccatgta aagaagaaat gccttatata gaacaactgt
    29041 ataaagacta taataaaaat aatgatgatg tagtaatttt aggagtagct tcacctaatt
    29101 taggtagaga aggttcaagg gaacatgtag ttaacttctt aaaagaccaa ggttatacat
    29161 tccctgttgt acttgatgaa gatggagcat tagcatatca atatggaatt aatgcttttc
    29221 caactacatt tataattgac aaggaaggat atgtaactca gtatattcca ggagctatgg
    29281 ataaggcaac tatggcatca tttattgaaa atcagagaaa taaataaaag tatttaagta
    29341 taagtttata aatcaattta aggttacaaa aaagtgccta attgtaccac ctaaataatc
    29401 ttgttatagt aaaagtataa acaatacaag aaagggttga attttatatg aaaaaaacat
    29461 ttgatgctat aataatagga tttggaaaag gtggaaaaac attagcaggg gatttggcaa
    29521 acaggggatt aaaagtagct ttaatagaaa agtcaaacaa gatgtatggt ggaacatgtg
    29581 taaatgtggc atgtatacca acaaaaagct tagaaaattc agcaaacagc gtaaaaacaa
    29641 agaatataaa ttcttgggat gaagtacaag ctgaatatga gaaagctata gataaaaaag
    29701 aaactttaat tacaaaactc agagaagcta actataataa attaaattca aatgagaatg
    29761 taactatatt cacaggaatg ggaactttca ttgatgaaaa aacagttcaa gtcaaaactg
    29821 aaaatgaaat ttatgagtta gtagcagata atatatttat taatactggt tcaagaccat
    29881 ttatacctaa tattaaggga attgaaaata agaatatagt atatgatagt gagtctctta
    29941 tgaatctaag aactttacct aaaaaaatga caattatagg agctggattt ataggtttag
    30001 aatttgctgg tatttatagt tcatttggag cagaagttac aatattaaac tctaataatg
    30061 gcattttacc aaatgaagat gttgaggatt cagaagaaat aataaaatta cttgcaaaga
    30121 gaaatgtaaa aattgttaat aatgcaaata taaaagagat taaagaagtt tctgaattag
    30181 caatagtaga atatgaagta gatggtaaat ctaaagaatt aacaagtaat atgatacttg
    30241 tagctacagg aagaaaagca aatactgagg agctagggct agaaaatgca ggtatagaat
    30301 taaatgaaag aggatttatt aaagtctcag aaactcttaa gactaataaa gagcatatat
    30361 gggctatagg agatataaat ggaggtccac aatttactta tatctcattg gatgattatc
    30421 gcatagttat taatcagttg tttggagata agactagaac aactaatgat agaaagaata
    30481 tcccaaattc tatatttata agtccagcat tttcaagagt tggtcttaat gttaaacaag
    30541 ctaaagaaaa gggatatgaa gtattagtgg caaagatgcc agtagaagca attccaagag
    30601 ctaaacagat tggcaaggca gatgggttta taaaaatagt gatagataaa aaatccaata
    30661 agatacttgg agctagtatg atatgtgaaa attcaagtga gattatccat cttattcaat
    30721 tagctgtaga tttagaagta gagtatacat atttaagaga ccgtgtttat gcacatccaa
    30781 ctatgacaga agctcttaat gatattctat ctcctaatat gataaaagaa gtataacata
    30841 agtttaatat ataaacctaa aattatcatt ataagtagat tttagaaaga ggctatctca
    30901 aaaaatagag ataagtctct tttttactgt ttaaatgata aaaatatatt tgattttata
    30961 aaagaagatt atcttatgaa ctaaaaagtt tattttaaaa cactacctat ttatgttata
    31021 aacttataaa tataatatag aatctattat tctgtattat taagtggggt taagcgttat
    31081 aattaattat aaatattgaa taatctaaat ggataagata atggtattaa aaattatctt
    31141 aaaaggtgaa ataatctgta tttaatgtag tacagttata tctttaaagc aaggtggtga
    31201 aatgatataa ataagatatc taaaagaggg tttgatgata gtgataaaag atggttttca
    31261 agtaaagaat taacaagact tataaagtct aaagaagaaa tagaatggtt attaaatagg
    31321 gattacaaaa tagacccagt cgttactttt gttggtgata gatatcagtt ttctataaga
    31381 caaagagatg cattaaaaag ggcagtctgt actgatgaaa aaaatattat aagacaatca
    31441 aaaaggcttt ctctagataa aatcaaagaa gataccgtaa atatagatgg ctttaatttg
    31501 ataatatcca tagaggtagc attatctggt ggtactctag taataggaag tgatggcaat
    31561 ataagagatt tagcaggtct taggggaacc tataagatta tagataaaac agaagaagct
    31621 ttgaatctga taggtaaatt ttttaataaa tacaatgcac agaatcttaa attttattta
    31681 gatgctccag tttctaattc aggaaattta aagtatagga tattagagca tgccaaaact
    31741 tggggaattg aaactgaagt agaattagta aaaaatgcag atgttgtatt ggaaaaatta
    31801 gatagagtag ttagtagtga tgcagtcata gttgacaagt gtataagtta ttttaatgtg
    31861 gctagaggta taattgaaga gtatattaaa gaatgtaata tcataaattt aaataaatag
    31921 ataaatatag gtgagaatat ataagtatat gtagcaatat tcttagtaat acaagtaaag
    31981 tttgaaaata ggagaacgaa tacatatcga atttcataaa atgttgtaaa aagacatatg
    32041 agaggatgga tatgtgtgaa attaactaaa ctaaatatta aaaaatatag agcacctata
    32101 tataaatatg ctttagcaaa tgtaaatttg aggtctgcta aatctactaa ttcaagtatc
    32161 ataacagtaa tacctcaggg agccaaaatg gaagtattgg atgaagaaga tgactggatt
    32221 aaggtgatgt acaattctca agaaggatat gtgtataagg atttggtatc agtaagcgaa
    32281 tatgcttgga gtaacttaaa cttaagagaa gataaatcta caacttccaa tataattaca
    32341 gtaattcctg aaaaatctag ggttgaagtc cttcaagtag atggagattg gagtaaggtt
    32401 gtatatgatg ataaaatagg atatgttttt aattattttt tatcaattga tgggaataaa
    32461 ccaaatgaat tagattataa gtatttttat actgacatga ttaaatttgt taacgaaaat
    32521 aacattaaaa gtactagcga ttatctaatt gtaacagacc ttagaaataa atatacttat
    32581 atttttaaga aagataatgg aggatgggga caactttaca agtggcaatg tactataggc
    32641 aagcctgaga ctccaactat aacaggtata ttttatataa gtggaagaaa accttctttt
    32701 ggaacggatg agtattcagt taagtatgct actagaatta aaggtggata ttactatcat
    32761 tctgtactat atgattcaac aggaagttat ataatagatg gcagacttgg tgaagctctt
    32821 agtcatggat gtataagatt aagtacggaa aatgcaaagt ggatatatga taatatacca
    32881 gatacaacaa cagttataat tcattaagaa aaattttgag ttttttattg ggataataat
    32941 tcttaaatta atagaattat atattaactt ctcataaaga gtactattta aaaagtgtaa
    33001 taagagagtg ctattaacaa aacaaagata ttttgaggca ctctcttata aatatacata
    33061 attaagatat tttcataaac aattctataa tcttatcttt atcaaagaaa acaggattag
    33121 caccagcaca agcatctttc atagagtttt ctgcaagcaa ttcaaaatta ggattatcaa
    33181 cacctacatc ttttaaagat tttgggattc caacctcttc agaaagagct tttatcttat
    33241 ctattacaaa gtcaacacac tctttatctg atttattatt tacatccatt ccaataactt
    33301 cagctattgg tcggaatttg gcaggggctg atttagcatt ttcctcctca actattggaa
    33361 gtagcatggc attacatact ccatgaggta aatcatataa acctcctaat tgatgagcca
    33421 tagcatgtac atttccaaga cctgcattac taaaggcaat tccatttaaa aaacaagcat
    33481 aacacatttg ttctctagct tctatgtcat taccattttt aacagctctt ggaaggtatt
    33541 taaatatctg ctttatagca tataaggcag ttgaatctgt aacatcataa gcaccattag
    33601 ctactacagc ttctatagca tgagttagtg catccatacc agttgctgct gttaaattag
    33661 atggtttgct aatcattaat tctgggtcat ttactgtcat tgtggctagt gaattggtat
    33721 caaccattat catctttaca tgtctgtctt catcagttat aacatagttt attgtaactt
    33781 cagcagatgt accagcagta gtagttatag ctatgatagg aagacatttt ttagaagttt
    33841 tgttgatacc ttcatagtct ctaatatctc ctccattagt ggctaatata gagatagctt
    33901 taccacagtc ttgtggagaa ccacctccta tagttataac gaagtcgcaa ttttctgatt
    33961 ttaatatttt aagtccttta ttcacattag ttacagttgg attttgttta acttcatcat
    34021 aaacaacaaa gtctacatct atctgagata aaatatctgt tacttttttt acagtaccat
    34081 ttgaaactag aaatttatca ctaacaataa atgctttttt agttcctaat gatttaattg
    34141 aatctttagc ttcattaaga catcctttcc caagtaaatt tattggtggt acataataaa
    34201 tgctcataac taaaatctcc ttttattttc attgattttt atgtaaaaat atatatctaa
    34261 caattttcac taacttactc ataatatatc gtatcttaga ttgagatagg attgttggtt
    34321 cttatataag aacttaattc gtaattacga ttatgattag aatttaacat agtattttta
    34381 tcatgtcaat aaaaatataa aaaatattat aaaaaaaaca aagttactgt agttaaatca
    34441 gcaactttgt ttttatagta taaatcaaat aaatacatta gtttaaaaca aaatttttaa
    34501 ttattatgtt ttgatatctt ccatatacag aatattaatc taatttatta caatgtcttc
    34561 attacgttct tccatcattg attgaatatt tttctgagca tttaagagta atttttttat
    34621 cagttctatt ttttcgtcat tagctctaaa taaaggcagt gttacactaa cagcagctct
    34681 tatacatcca ttttttctta ttggtacggc aagacatttt aggtgttcca ttgattcctc
    34741 acactctgaa gctatttgtg ttctttgaac ttcaagaagc tggtcgtata aaacatcaaa
    34801 atctgtaatt gatttttctg taaaaccttt tagaccatct ggatataagc ttattagttt
    34861 ttccttgtca aaatcacata aaagagcttt tccaagacct gtacaattag caggtatttt
    34921 ttttccaaca taagaaataa gtctaattgg ttctggagaa tctacttttc caatataaag
    34981 aacttcatta ttgtcaagta taccaagctg acaagtttca gaacaggaat taactatatt
    35041 agtcatttcg tcaattatat agttaaaaat agttcttttt tcaaaaaatg aagaccctaa
    35101 tgtgtaagaa cgtatcccta tagaatattt ggatgtgttt ttatcaatag taatgtattt
    35161 tctagcatta agtgtatgaa ctataggaaa gatgctactt tttggagcat tgataacatc
    35221 tgctatttct gtaagagtat atccattttc ggattctgct aatgcttcta aaatatctaa
    35281 tatcctagct gttgggcgat gattatctga agtcataaat ttccccctct ttttattaat
    35341 ttaaagtatt agaatatgtt gattttagca cactatacga tattttacaa tgtagaaaat
    35401 aaatatgtaa atttatatat aaataagctc ttgatttaga tacagataga tattttttta
    35461 gactgtacat taaggaataa ctaatttcca aaatgtacct aaagaaa
//
LOCUS       DAYAPA010000031        34748 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.031, whole genome shotgun sequence.
ACCESSION   DAYAPA010000031 DAYAPA010000000
VERSION     DAYAPA010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 34748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34748
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.031"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(441..2828)
                     /locus_tag="ACGI59_001389"
     CDS             complement(441..2828)
                     /locus_tag="ACGI59_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFG9739182.1"
                     /translation="MEANNSIKKEFILGGLNCAHCAEEINNKVSKLQEVKSSNLNFIN
                     KKLTVNIKESFNEDTTIEKIIDIIDSTEPGLDIQISSKENAASKTSIKKELILGGLNC
                     AHCAEEINNKVSKLKEVESSNLNFVNKKLTVNISNNFEEDDVINKIKEIINSTEPGLD
                     IQVGSTDKVKGRTTEKSGAVNDTNKKELIPLIIGALVYIFGIYQTATGYESQFSNIVF
                     IVAYVIVGGDVLLRAIRNISKGRVFDENFLMALATVGALAIGELSEAVGVMLFYKVGE
                     YLQGVAVGKSRKSITSLMQIRPDYANLKVNSEVKVVSPEEVNVGDIIVVKPGEKVPLD
                     GVVVDGVSMLDTSALTGESVLREVEKGDEILSGVINKNALLSIEVTKSFGESTVSKIL
                     DLVENSSIKKSKTENFISKFSRYYTPIVVIAALLIAFVPPLVISGEVFSDWLYRGLIF
                     LVVSCPCALVLSIPLSFFSGIGFASKNGILIKGSNYLEALRSVDTVVFDKTGTLTKGV
                     FNVTKLNPEGISEEELLEYAAIAEVNSNHPIAKSILSYYNKKIDLDTIDSYEEIAAYG
                     IRVKHNGNFILAGNEKLMKKENISYSSAKEVGTVVYIAVDKAYRGYIVISDEVKEDSK
                     NAIRSLKEIGVKEVVMLTGDNEKVAKNIAQELELDTVYSNLLPNEKVDRLEDLYEGRT
                     EKEKIAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVLMTDEPSKISKAIEIA
                     NKTNKIVWQNIIFALGVKIIVMILGAGGVATMWEAIFADVGVALIAVVNAMRAMR"
     gene            complement(2851..3216)
                     /locus_tag="ACGI59_001390"
     CDS             complement(2851..3216)
                     /locus_tag="ACGI59_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFG9739183.1"
                     /translation="MREEINDCNCNIVHEEIVTEAKSTMPDEEMLYDLAELFKVFGDT
                     TRVKILYALFANEMCVCDIASLLNMTHSAISHQLRVLKQARLVKFRREGKTVYYSLDD
                     SHISQIFDCGLNHIRETYK"
     gene            3415..4263
                     /locus_tag="ACGI59_001391"
     CDS             3415..4263
                     /locus_tag="ACGI59_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9739184.1"
                     /translation="MSNKKKKDLDTSYMPYLDPANYADYITNEEIPQDELRNDPQISN
                     GYTKIPNNQNINNLYPNNVNHYPKSSNSPNNLPNTNNTSGNMNYNTSATNNMPNNMNN
                     NTGMPNNMSNNMNSNMGMPNNMPNNMNSNMGMPNNMPNNMNSNMGIPNNVPNNMNSNM
                     GMPNNMSNNMNSNMGMPNNMSGNMNNNMYGNTGMPNNMNSNMGYNMNNTMGIPNFSCN
                     QNMPPNVLMMIPGVICHNTMQGMPVVMPSTMPPNIYPTPYGSSNMSIQGIPQATNIEE
                     FDEEEM"
     gene            complement(4700..5788)
                     /locus_tag="ACGI59_001392"
     CDS             complement(4700..5788)
                     /locus_tag="ACGI59_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9739185.1"
                     /translation="MNFNFAKATNSRLMGSLGLIINWIDDENNHFCQYFLLDAEGLGL
                     ADYVSLNNPTQEEAYMEEERLMGGFGSDRVELTKDESLFLVSYFGNKNLYYDKLLPGD
                     KCEYIDIIKNYKTDLTIEKLYNKICKRVDEEVEFINYMTMRFIAWDRESLKYFSGSDE
                     IANMHITNINGTLLKNVVSDKGQGRYISEALYEDNDGYYICKIAFCISKCYETGFRIN
                     SLLVTDKEPMYDFEVFDEISKSEFVSVYSVNSPEEFAGVFYRENPFLLKSNMNEGIFF
                     TRFNFNNDHVKENVYIINNDMKAIYYLMGNKFFIGTYNENDCNYINEISQSNYSDYIK
                     FEEKFFFEQNALYDFAESGSLDFDDFLE"
     gene            5913..6641
                     /locus_tag="ACGI59_001393"
     CDS             5913..6641
                     /locus_tag="ACGI59_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFG9739186.1"
                     /translation="MKNIAAFFDIDGTLYRDSLMVEHFKKLIKYDIIDQKNWYKHARD
                     TFMDWDKRQGNYDDYLLEICDLYVDSLKGLDKTCINFTSDQVIKLKADRVYKYTRSRI
                     EWHLNQGHIVIFISGSPGFLVEKMAKKYNVTDFLGSDYVFENNIFTGTVIPMWDSISK
                     NNAINDFVVKYDLDLSNSYAYGDTNGDINMLKRVGNPIAINPTKELLSQISLDEEISK
                     NVEIIVERKDIIYSLSTDVNIVDI"
     gene            complement(6843..7334)
                     /locus_tag="ACGI59_001394"
     CDS             complement(6843..7334)
                     /locus_tag="ACGI59_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9739187.1"
                     /translation="MSIKVAYGGVLLALNVILLTLTNIIPVNTLFIMGLASLLVSIVI
                     MEWGFKSGIAFYIGSIVLGFIVMASKSQWIVYSLTFGIYGIVKYLIEKDRSIYIEYFM
                     KLVFANIMILILYFLLRTIVYIPINIFIIGSFEIAFIVYDYVYSSFIGYYNNRLRKML
                     FKK"
     gene            complement(7345..7788)
                     /locus_tag="ACGI59_001395"
     CDS             complement(7345..7788)
                     /locus_tag="ACGI59_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9739188.1"
                     /translation="MYSNTYNNENFMRARAFIENSEFKKAYDFLKTLTDKCAEWYYLT
                     GFSAMNIGYYEEGEDFLKRAKFMEPENSEYSDALRSYTQYRNDYSNRADNYNRRRRND
                     LDGCCCCCCDDCCCCLGDDCCENCAKLWCLDSCCECFGGDLITCC"
     gene            complement(7778..8617)
                     /locus_tag="ACGI59_001396"
     CDS             complement(7778..8617)
                     /locus_tag="ACGI59_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5685 family protein"
                     /protein_id="HFG9739189.1"
                     /translation="MFGYVKINKMDLTFREYDYYKAYYCGLCKYLKRNHGEISRFSLN
                     YDITFLIVLLTAVYNPESISTEEVCIVNPFKKKKVITNDITEYAASMNILLTYYKLED
                     NLMDDKRIKDKLAYYIYKNKLKLAYEKYPEKAEYIKQQLNELNKLEKDKNINIDEVSS
                     IFGNIMGEVFVYKKDENERNLRMIGFNIGKYIYLLDAYEDLDEDFKKGRYNPFIEYID
                     KNDELKEKVKKIIVTSLGFLARGIDNLNLNKNVGIIENIIYSGVYLRYINILESRGGK
                     NVQ"
     gene            complement(8836..10221)
                     /locus_tag="ACGI59_001397"
     CDS             complement(8836..10221)
                     /locus_tag="ACGI59_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFG9739190.1"
                     /translation="MENLNKKFLMEYIDEIQGELRDISDKIWKNPELQYKEYYASNLQ
                     KEYLKKYGFSIDELEGMDTAFAASYGEGRPVIAVLGEYDALDSLSQKVSTIKEPIVDG
                     GAGHGCGHNLLGTGSIGAVIAVKELIKLKKLKGTIKYYGCPAEEDLSGKVLMIKKGFF
                     DGIDCAFSWHPFDINTPIRIPTLANYSVKFRYNGISAHAAQAPYNGRSALDAVELMNI
                     GCNYLREHIFDNCRVHYVTTNGGKMPNIVPDFAEVWYYIRGVKMEHVRDVFGRIVDIA
                     KGAALMTGTIMEYDIISGVYDYIPNTILTDILSQNMKLVGVQNYNEDDYYFADKLAET
                     VSIDKRSSVSSVLSGNKDITKMNLHDEVTDDTFTHNNCISLSLDIGDVSYIIPTAQCS
                     CSVWPIGISAHTWQSCASAGSDMGFKAMLLASKSISCSINDVMLDELVINKAKKELKD
                     TVGSFKYIPII"
     gene            complement(10394..11137)
                     /locus_tag="ACGI59_001398"
     CDS             complement(10394..11137)
                     /locus_tag="ACGI59_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HFG9739191.1"
                     /translation="MKKVLIVGESWVKNITHIKGFDTFVTTHYEEAVKWLKEAIESGG
                     YETVHMPAHVAADSFPYKLEELNEYDCIILSDIGSNTFLLSNSTFIDCNSNPDRLELI
                     KEYVNNGGALIMVGGYMSFTGIDAKARFGETAIKDVLPITMIDKDDRVEKPAGIIPEV
                     IDSKHPVLKGIPTEWPKFLGYNKTVARDNCPVLATIGGDPFVAVGEFGKGKSAIFSSD
                     CAPHWGPKEFTDWKYYNKLWVNMLDWLTC"
     gene            complement(11157..12419)
                     /locus_tag="ACGI59_001399"
     CDS             complement(11157..12419)
                     /locus_tag="ACGI59_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFG9739192.1"
                     /translation="MKKILLDTLNSKKQEYIDYLKELVSIKTEDVGHGILGGFEKEGQ
                     EYIEKLANYIGFSVDRQEMSEELIKKAKNIYKEGNLGHNYQDRYNLICKYSDDLPGKT
                     IVFNGHVDTMPPGDISKWKYNPYRATEDNGKLYGLGTADMKSGLIASILAVKLIKDSG
                     LNVPGNVKIMSVVDEEGGGNGTINAVMNGIDGDCCIICEPSEQNLIVAHMGFVFFEVE
                     VKGVSLHCGSKWEGVNAIEKAMLLLQDIKELEHNWLMIYKHPLLPSPTINLGVINGGT
                     AGSTVPDKCVFNLCVHFLPNIMSYEQVVNDVTNVIMTRANGDLWLKDNKPNINIYQSG
                     LGFEMDKDSDFVVNAHKILEETLGKKLEIKGSTAGNDARLMKNLAEIPTLILGPGSIE
                     QCHSIDEYVEIKEYLDSILMYASLILNL"
     gene            complement(12603..13601)
                     /locus_tag="ACGI59_001400"
     CDS             complement(12603..13601)
                     /locus_tag="ACGI59_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9739193.1"
                     /translation="MSSITKHKEEKSSPLLTKLTSVFRDQGSAAVGLVIIFIIMSVAS
                     SNFLTLDNLINVGRQISINAILAVGMTFVIITGGIDLSVGAVIALVGTFWATTVVNYN
                     APIWVGMILALAIGTILGVIKGAIISTQKLPPFIVTLAMLTIISGASFVFTGGRPISV
                     NTDAFKMLGRGYIGPIPIPVIIMIIVVIAGHFLLKRTNFGRHVHAVGGNEEAARLCGV
                     KVNKVIVKVYALAGLLTALAGIILSSRLASGSPTVGDGAELDAIAAVVLGGTNMMGGS
                     GSIVGTCIGVGIIGILGNGLNLLSVSSYNQMIIKGLVMLFAIWINNIKLKKSAKSK"
     gene            complement(13585..15096)
                     /locus_tag="ACGI59_001401"
     CDS             complement(13585..15096)
                     /locus_tag="ACGI59_001401"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFG9739194.1"
                     /translation="MSNIILKLSNIAKEFPGVRALDNVNFELFHGEVHALLGENGAGK
                     STMIKILTGAHSKTSGKFIFEGKEIENISPDISKKIGINAIYQELTVFDELTVAQNIF
                     MGKEINGKVLTNDKKMNEEAKKIFDNMGIDINPNSLVKELSIAQKQMVEIARVLSSET
                     KVLIMDEPTSSISKKETEILFRLINDLKESGVSIIYISHRMEELFEICDRITIMRDGK
                     TISTLNTKDVSSEEELVNLMIDRKLDQFFPKRKVEIKEEIMRVENLTKNNVFNDISFN
                     IRKGEILGIGGLVGSKRSEIVEAIFGLRTYDSGKIYLNNEEVKFKTPSDAIENGLGLI
                     TEDRKGTGLFLQMSVKENTTMAGLKKISKFKSIIDRKKEKEILEKYIEALKIKTPHMN
                     QVIQSLSGGNQQKAIIARWLLLQPDILIMDEPTRGIDVNAKAEIYNLMGDLVESGVSI
                     IMISSEIPELISMSDRIMVMREGHISGFLEGEEMVENNVLKLAFGGKINEFNN"
     gene            complement(15150..16112)
                     /locus_tag="ACGI59_001402"
     CDS             complement(15150..16112)
                     /locus_tag="ACGI59_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFG9739195.1"
                     /translation="MRVRKILSLGIAGILAIGMLTGCSMEGPSKSDNKGGSDKKDLTI
                     GVSTITLQHQFFIDIDEGIKEKAKELGVKVIVNDPDQDVAKQTSAIEDFIQQNVDGMI
                     VLGTDNSAIVPAVEGAFEKMPVVTVDAVLNTENITSYVGTVSYDAGKKLGEYTKKYID
                     EKLGGKSEIAIVTDLKSQIQMQRIDGFKDALKGSANVKILNSQPGYDREESLNTVENL
                     IQSNPDVDIIYATAENSVLGAKAALESAKNKDVKIVGFDLTEEASSGITDGTILAMIQ
                     QQPKEMGRLAVEAVVKAIKGEKVEKNIPVPALLYDKENIKDFKN"
     gene            complement(16160..17050)
                     /gene="rbsK"
                     /locus_tag="ACGI59_001403"
     CDS             complement(16160..17050)
                     /gene="rbsK"
                     /locus_tag="ACGI59_001403"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFG9739196.1"
                     /translation="MGNIVVIGSVNMDMVCSVDKRPEKGETVLGNSFFTSPGGKGANQ
                     AISASKLGANVKMISCIGEDGLGEELIRNFRTNKVDYSLVSRNNHKSSGVAVITLCEN
                     DNSIVVVPGTNELVDIELIKKNEEEIKNADIVLLQLEIPLKTINYVVNFCFENRIRVL
                     LNPAPAVKLNEDIIEKVTYLTPNEHEYKIVFDTNEGIEEVLKKYPNKLVITEGKNGAR
                     FYDGEEIKHVSCISVDVQDTTGAGDTFNGALAVAITEGKNLYTAVEYAVVVSGLSVTK
                     LGAQSGMPYKEDVEKYLNNK"
     gene            complement(17092..18114)
                     /locus_tag="ACGI59_001404"
     CDS             complement(17092..18114)
                     /locus_tag="ACGI59_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFG9739197.1"
                     /translation="MNFLSITIKEIGELAGVSKTTVSKVINNKDENISQATREKILKI
                     MKEKNYVPNKLAQSLVTKKTNTIGLLIPDIRNPFFTDVSRGVEDKANEEGYNIILCNT
                     DEDAKKEYEGIRTLSERMIDGIIFAASSNTNWKEANYKDIKIPTVLIDKKISMNKETL
                     KGIVKINNFEGAYIATKHLLDIGNKKIIYLSGPLQNEIAVDRLEGYKKALIECNLSYN
                     PAYVFEGKYKIEWGQEFIKNLEKIDFDAIFCANDLIAIGVIRGLKERGLSIPNDISVV
                     GFDDIQTSSLISPSLTTVKQPSYDIGYKASEILINCLRGDKKESFDELIFKPELVIRD
                     STKESE"
     gene            complement(18334..19311)
                     /gene="bioB"
                     /locus_tag="ACGI59_001405"
     CDS             complement(18334..19311)
                     /gene="bioB"
                     /locus_tag="ACGI59_001405"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HFG9739198.1"
                     /translation="MEKYIIKLKNKVLREKEISYEEALNLISLDTNNKNDFDILLKSA
                     NEIREYFMGRKADLCTIMNAKSGKCSEDCKFCAQSSHYKTGVEEYSLLDYNEILNRAK
                     EMESKGVHRFSLVTSGKGMSGKEFNNILNIYEGLRENTNLKLCASLGIIDYEQAKMLK
                     SAGVTTYHHNVETCRDNFHNICTTHTYKDRIKTIKDAKKAGLDVCVGGIIGMNESEEQ
                     RLKMAFEIRELNVKSFPINILNPIKNTPMENYDVLEPMEILKTTAVFRFIIPNVYIRY
                     AGGRLSLKGYDKVGFNGGVNSAIVGDYLTTVGSGIENDKKMIIEQGFEL"
     gene            complement(19517..19780)
                     /locus_tag="ACGI59_001406"
     CDS             complement(19517..19780)
                     /locus_tag="ACGI59_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9739199.1"
                     /translation="MKIYYEKENSKKANVNNIPSVFAFNFLRNFLTSKEYEETRKEYF
                     INNLTKSQVNQAMKDLKWLFREYEGLEVITMESLDGVKTRIVL"
     gene            complement(19850..20923)
                     /locus_tag="ACGI59_001407"
     CDS             complement(19850..20923)
                     /locus_tag="ACGI59_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HFG9739200.1"
                     /translation="MEKSKVYFCDLYSNSQNKNVPNNVRRLFDEAGFKDLIEKNDQVA
                     IKLHFGEKGNTTYMSPVAVRQVVDKVKDCEGKPFLTDTNTLYTGSRTNSVDHLTTAIE
                     NGFAYAVVNAPVIIADGLYSRNYENVKIDKKHFESVKIGGEIYNSSAMIVMSHFKGHE
                     AAGFGGALKNLAMGCASAAGKQMQHSDVTPVVKEKKCVGCGKCVNSCPTKAISIVDKK
                     AVIDSDVCYGCGECPTVCPTRAVTIQWESDSDVFVEKMAEYAYGAVSNKKDKVGYITF
                     VMNVTPLCDCVPWSGRPIAHDIGILASTDPVAIEQACYDLICKEMGHDVFKHEHPHVN
                     GTRIIDYACEMGMGSKEYELIKL"
     gene            complement(21171..21929)
                     /locus_tag="ACGI59_001408"
     CDS             complement(21171..21929)
                     /locus_tag="ACGI59_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFG9739201.1"
                     /translation="MREYIAFTKKEFKENLRNYKLFSLIILFLIFGISSPISAKFMPD
                     LIAHFAPTLKVTAAPTALDSWTQFFSNISGLGMSLTLIIFCNILSNEYSKGTLVIMLT
                     KGLSRSSIVLSKFSVTVIIMTIGFWLSFLFAYGYTIYFWPTANLNHIIFSAFNLWLIG
                     IMYISVLILGCVLFRPAFASVLLVLVATLLLSLISIPKQIAPYTPNFLMSKNIDLISG
                     KVAMPEFIIPIIITVLISVVCLLAAVILFNKKPV"
     gene            complement(21926..22846)
                     /locus_tag="ACGI59_001409"
     CDS             complement(21926..22846)
                     /locus_tag="ACGI59_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFG9739202.1"
                     /translation="MMNIVSINGLSKGFGNRKIIDNLNFTVPEGSVFGFVGKNGAGKT
                     TTMKMVLGLLKPDSGTIDVCGEKVTYGKTSSNRHVGYLPDVPEFYNYMRPLEYLSLCG
                     EITGLSKKEIQIRSEELLSLVGLGNEKRRIGGFSRGMKQRLGIAQALLSRPKLLICDE
                     PTSALDPVGRKEILDIMLKIKDSTTVIFSTHILSDVERICDHVAILNKGSIALSGTLS
                     EIKSMHGKDRLLLEFASNDEIQKFKSSDGIKSLLKDSEETNMEIVLHGKDIKAIQKTV
                     ISTLAEMNLCPVKMELIELSLENLFLEVVK"
     gene            complement(22843..23043)
                     /locus_tag="ACGI59_001410"
     CDS             complement(22843..23043)
                     /locus_tag="ACGI59_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLD nuclease N-terminal domain-containing
                     protein"
                     /protein_id="HFG9739203.1"
                     /translation="MENLMEYLPLLVPVIILDLILIITALVHVLRHPNYKIGNKAIWI
                     IVVLFISLIGPILYFTIGRGEE"
     gene            complement(23048..24142)
                     /locus_tag="ACGI59_001411"
     CDS             complement(23048..24142)
                     /locus_tag="ACGI59_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFG9739204.1"
                     /translation="MNELNIAKTLILKRKEKGITQDELANYIGVSKASVSKWETGQSY
                     PDITFLPQLATYFNITVDELICYEPQMMKEDINKLYNKLCKDFTVKPFDEVMDEIREI
                     IKKYYSCFPLIFRMGLLIVNHYDIVDEKKRELLISEALEIFIRIQETCNDIDICRQAK
                     SMEATCYILLNQPIQVIDLLQNSNFPMINESILLAQGQMMNGQMDEARETFQLGAYQN
                     LISLVQNLVGILQNADKLQMKEIERRILAISDIFELDTLSPAIMLSSYLTQAQINLIH
                     GDNEGAIKSLRKYVDLATRDIYPIIIHGDDFFNKLDRWISEIGTGITRDDTIVKAGIV
                     AAIKNNPMFSVLSENKEYKFLIEKLSLLEE"
     gene            complement(24652..24792)
                     /locus_tag="ACGI59_001412"
     CDS             complement(24652..24792)
                     /locus_tag="ACGI59_001412"
                     /inference="COORDINATES: protein motif:HMM:NF021468.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2185 domain-containing protein"
                     /protein_id="HFG9739205.1"
                     /translation="MNGEGCILSNKITKECYKVGYMIREKSIDTYPDSGWGFMTWDEN
                     GE"
     gene            complement(25102..26409)
                     /locus_tag="ACGI59_001413"
     CDS             complement(25102..26409)
                     /locus_tag="ACGI59_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFG9739206.1"
                     /translation="MDISRSVDNSLNRTIEFLKELIKIDSQQGEPISQCPFGIGPKKS
                     LDKTLDYCASLGFSVKNIDNYIGYAEIGEGEELIGIPMHLDIVPPGEGWSVDPFSGAV
                     IDNIIYGRGVIDNKGAVSMLIHVLKNIEDMYPTINKRIRLIFGTNEETGMKCIKYYLD
                     KGEEIPSMGFTPDAMYPVVNGEKGRVHIRIEKEIKIDKSKPYIIVRGGTKENVVPSHC
                     TAKIINGIISELTTKGVAVHASNPEKGENAISKMVIKIVEDNMDFQHREDIELVSKYL
                     CSDYYGDALGINQYDEVFKNTTLNLGILKVNEEKIVCELDIRYGKNIVLNNIIDRFKK
                     VFCNGWKIEVIAHKDLHYVDESNLVLKKLLEAYEEVTDENGYTIAMGGGTYASWFKDM
                     VAFGPKFLAYKTGGHGVDERVPINHIRKNMEIYTLALIKLLEL"
     gene            complement(26474..26953)
                     /locus_tag="ACGI59_001414"
     CDS             complement(26474..26953)
                     /locus_tag="ACGI59_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HFG9739207.1"
                     /translation="MSEKMYTEKEYLEGIRGATGERAVWFYLLMKEAEKLGANPDDIC
                     KEAIYGFGKMRGQKYNVADTPGKMAEMLYNSKGQKVFEMELVENTDENGVLKFHHCPL
                     DAAWKEYGLTKEERKEICRLACYGDYGRVDCAQGVKLDFAQKCAHDDEVCELVFTKK"
     gene            complement(27000..27863)
                     /locus_tag="ACGI59_001415"
     CDS             complement(27000..27863)
                     /locus_tag="ACGI59_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFG9739208.1"
                     /translation="MQEIKNKEVKNGQGPTEVNKTRVLTKKDVTKTYLRWWWTAELSN
                     SFERMQALAVCASFTPALEKLYKRKEDLVDALKRHLQFFNTQAIWGGLIHGTVLAMEE
                     EKATEGKIPGEVISGVKNGLMGPLAGIGDTLDFGTFQTIFLALGASFGAEGSVIGAFF
                     PIMFSILLFCEGYYLFHLGYSLGRDSIKKILSGGIVNKIIDGASILGMFMMGALSATT
                     VKLSTPLSFDIGGKAIVVQDTLNMIAPGLLPLGVVFFVYWGMKYKKWTITKLLVILVV
                     LALVGSFIGIF"
     gene            complement(27863..28618)
                     /locus_tag="ACGI59_001416"
     CDS             complement(27863..28618)
                     /locus_tag="ACGI59_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFG9739209.1"
                     /translation="MESVLMLAIVTGLWYWFAAGLAGYTLFSTLKSPLFIGFSLGLLW
                     GDVTTGMIVGASIEMVYLGMVAAGGNIPSDKCLAALIAIPVALQTGVNAEVAVSIAVP
                     LGVIGVLVNNLRRTGNAVLVHKADKYAEEGNTKGIWRCATLYSLIFGFVLRFPIVFVC
                     NFFGADLVQSLLDVIPQWLMNGLTVMGGILPALGFATTIFTIGKNKFLPMFIIGFFMV
                     QYFEISITAAAIFGVCIALLITFMKEDKRVGEV"
     gene            complement(28645..29133)
                     /locus_tag="ACGI59_001417"
     CDS             complement(28645..29133)
                     /locus_tag="ACGI59_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFG9739210.1"
                     /translation="MAKITLVRVDHRLIHGQVITKWVKIAQAQKIIIVDDFLGQDEFM
                     ADIYKMAAPSGVEVVILTAEDAGQAFQNNTLGDKNIFILFKNVDMANKAYKAGLKYEK
                     IQLGGIPNEAGKKMVFTAVSLGNEDVEQLNELNENGVEIVLQVIPEESSMTYENALKK
                     FK"
     gene            complement(29151..29552)
                     /locus_tag="ACGI59_001418"
     CDS             complement(29151..29552)
                     /locus_tag="ACGI59_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFG9739211.1"
                     /translation="MNKYKVIILCHGTWGKVLVEETNKNFGLSNQYEVLSLSSEKDVS
                     VFMKEVEEVVDKEDVKIIISDLYGGSTSSVAIAVAIRRGIDAINGLSLQTILIVDEEL
                     SKEDKLHTLPERIVKKNNNLCVDLIKEFKKI"
     gene            complement(29555..30052)
                     /locus_tag="ACGI59_001419"
     CDS             complement(29555..30052)
                     /locus_tag="ACGI59_001419"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFG9739212.1"
                     /translation="MSLVKYCRCDDRLIHGQVIYKWVKHLGVKKIVVVDDETTNDVIA
                     KGLIKMAAPKNIDLSILTVSESRRYFYNNQADDNVFVLIKNLDTANRMIEEGINIKKL
                     IVGRIPTGIGKKKISQNVYINKKEFLLIDEFIKKNINVSIQMVPDEEEVELNQNINRY
                     KGEFI"
     gene            complement(30076..30480)
                     /locus_tag="ACGI59_001420"
     CDS             complement(30076..30480)
                     /locus_tag="ACGI59_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFG9739213.1"
                     /translation="MIGIIVMTHGSFSEEIIKSCELIAGPAERTAAIKLNRNDNIEDL
                     NKNFVEKLNELDEGDGVLVLADLLGGSPSNVASLNLKKGGKFHALTGVNLPMLLEALI
                     NREGKSLEELAEACIEAGQTGINNINKVLSSM"
     gene            complement(30710..33277)
                     /locus_tag="ACGI59_001421"
     CDS             complement(30710..33277)
                     /locus_tag="ACGI59_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFG9739214.1"
                     /translation="MTQKKLSRKEKVLICLKRLTEEHLNFNNGIRFGFDAEYIATEVG
                     INRSNASKELNQLIKESIIIKIKGKPVQYLYKESIESILGKKLNKGIFDNINSINEYS
                     ENDSIKDEYILESIKKENNIFNLVGCDGSLRSQIEQAKAAVIYPPKGLSTLIIGPTGV
                     GKSIFAEYMYKYSLTIKEDSDNAPFIIFNCADYSDNQQLLLAQLFGYVKGAFTGADKD
                     KYGIVHEANNGVLFLDEVHRLSAEGQEMLFLLMDKGIYRRLGEANKVHECRVMIIAAT
                     TEDPETAMLETFLRRIPVTIKIPSLEERGFQERMKLICYFFKEESVRIGVKLKVSKEV
                     IKAFILYKCKGNIGQLKSDIQLICARAFLDYMTYKRECVYVRLSLLPNNIRESLYGNN
                     RKEEFVQNFSFIGKDDIIFLPEEKDFESENLLIENKKYDLDFYGMIKETWNKLQQEGI
                     KESQIRSVIDGDIQKYSYNLMNTFIYNSSNQTAYNNIVNDSIATTVKYILQKHDKWSK
                     REGLDKLIKAIALHIQNLIERIKIGNIVKHPNKDEIMKERAYEYNIAKEILSNMSLMY
                     GVEFPEDEAIFLATFLYLSYVGLNEESIAILVIMHGESTASSMVNVANTLLDCNHAVA
                     INMGLEDRVQDILVQAVEIANKIDKGKGILILTDMGSILTFAKVIRDATGKEVKAIDM
                     VSTPIVIEATRKALTPEMTLEKLYFNIIESIKKHYGDMEVTYAEKDNGGRYFDRLLID
                     NISKTLTFLNGEKAYFILKEVINRISEHYSLVIQDELLVKFIFHCSCMIERVIIKESL
                     VYKSYEERISRNKELYLVIKESFKLVEETFDIIISDMEYANILDIFESQYDTDKFKS"
     gene            33443..34186
                     /locus_tag="ACGI59_001422"
     CDS             33443..34186
                     /locus_tag="ACGI59_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HFG9739215.1"
                     /translation="MNFLSDLHTHSIVSGHGYSTLLENINYCKENGIKILGTSEHGPK
                     MPGSPHRWYFHNIQNIPRIINNIIILRGCEANILDIKGNIDLEPFVIPRLDYLILSFH
                     EAVFSPNTLENNTKALINAINKHDNIEILGHLGNPNYPIDYELIIKLAIEKNILIEIN
                     NCSIKGVSRNGSSDNCKYIATLCKKYGAKIILTSDAHICFDIGNYEYSENILKEINFP
                     DELIMNYPKKLINHFHQKGKLLDVDYTNI"
     gene            complement(34498..34704)
                     /locus_tag="ACGI59_001423"
     CDS             complement(34498..34704)
                     /locus_tag="ACGI59_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9739216.1"
                     /translation="MSKYSSLWEYVKKNNSQSFKLTFEEIKDIAGIEIDHSFLKYKKE
                     LNEYGYQVGKISLKEKTVIFDKIG"
BASE COUNT        11243 a         6368 c         3552 g        13585 t
ORIGIN      
        1 cttaaaaata attcgtcaat acttttttaa attatttttt aaaaatattt actttcttat
       61 attacataat ttattgaatg gcagtcatac tgttggttta gagtatttta acacacacat
      121 tttgttgtaa aaattacaac tattaatata atatatatac aatcataagt attataattt
      181 ttatgaatct aatttaaact taatataaac ttattaataa accttctatg tctaaatttt
      241 aaaattacaa tataaaagct ctacactaaa taaatacatt aaataaacat aatggataat
      301 gaatataaat ttatatatgc aaagcgctcc aataaaaagg taccaataaa gttaaaagaa
      361 acttattgat acctttcatt attacatact tcaatatata attataagtt ttttaaatag
      421 tatggattta catttatccc ttatctcata gctctcatag catttactac tgctataagt
      481 gccacaccta catcagcaaa tatagcttcc cacatagtag ctactcctcc agcacctagt
      541 atcataacta taatttttac tcctagagca aatattatgt tttgccacac tatcttatta
      601 gtcttgtttg ctatttcaat tgctttagat atcttgctag gctcatctgt cataagcact
      661 acatctgcag cttcaatagc agcatctgaa cctaagcctc ccatagctat accaacatca
      721 gcacgagcta atactggagc atcatttatt ccatcaccta caaatgctat tttctctttt
      781 tcagttcttc cttcatataa atcttctaat ctatctacct tttcattagg aagtaaattt
      841 gagtaaactg tatccaattc taattcttga gcaatatttt ttgctacttt ctcattatct
      901 ccagttaaca ttacaacttc ttttacccct atttctttta agcttcttat ggcattttta
      961 ctatcttctt ttacttcatc agagataact atataacctc tatatgcttt atcaacagca
     1021 atatagacta ctgtgcctac ttctttagct gatgaataag atatattttc ttttttcata
     1081 agtttctcat tacctgctaa tataaaattg ccattatgct ttactctaat tccataagct
     1141 gctatttcct cataactgtc tattgtatct aaatcaattt ttttattata gtaacttaat
     1201 atggactttg ctattggatg atttgaattt acctccgcaa tagctgcata ttctaataat
     1261 tcttcttctg atataccttc aggatttaac ttagttacat tgaacacacc tttagtaagt
     1321 gttcctgttt tatcaaatac tactgtatct acacttctta aagcttctaa atagttactt
     1381 cctttaataa ggataccatt tttagatgca aacccaattc cactaaagaa actaagtggt
     1441 attgataaaa ctaatgcaca agggcaagaa acgactaaga atatcaatcc tctatataac
     1501 caatcactaa atacctcacc tgagattaca agtggtggca caaatgctat taataaagca
     1561 gctattacta ctattggagt atagtatctt gaaaacttag atataaagtt ttctgtcttt
     1621 gattttttaa tgctcgaatt ttcaacaagg tcaagaatct tagaaactgt tgattctcca
     1681 aaactctttg taacttcaat actcaataaa gcatttttat ttataacacc tgataagatt
     1741 tcatctcctt tttcaacttc tctcagcact gactctcctg tgagtgcaga agtatctaac
     1801 atagaaactc catctactac tacaccatct aatggtactt tttcaccagg ttttactact
     1861 attatatcac ctacatttac ctcttctggt gacaccacct taacttcaga attaactttt
     1921 agatttgcat agtctggtct tatttgcata agtgaagtta tagactttct ggatttacca
     1981 actgcaacac cttgtaaata ctctcctact ttatagaaaa gcataacccc tactgcttct
     2041 gataactccc ctattgctaa tgctcctact gttgctagag ccatcaaaaa gttctcatca
     2101 aatactcttc cttttgatat atttcttatt gctcttaaaa gcacatcacc acctactatt
     2161 acataggcaa ctataaatac tatattgcta aactgacttt cataacctgt agctgtttga
     2221 tatataccaa atatatatac aagtgctcca attataagtg gaattaattc tttcttattt
     2281 gtatcattta cagctcctga tttttcagta gttctccctt ttactttgtc agtagaccct
     2341 acttgtatat caagccctgg ctctgttgaa tttattattt cttttatttt atttataaca
     2401 tcatcctctt caaagttatt acttatattt actgtaagtt ttttatttac aaagtttaaa
     2461 ttagaagatt caacttcttt taatttagaa actttgttat taatctcttc tgcacaatga
     2521 gcacagttta atcctcctaa tataagctct tttttaattg atgttttact agctgcattt
     2581 tctttagaac ttatttgtat atcaagacct ggctctgttg aatctattat atctattatc
     2641 ttttctatag tagtatcctc attaaaactt tcctttatat ttactgtaag ttttttattt
     2701 ataaaattta aattggaaga tttaacttct tgtagtttag aaactttatt attaatctcc
     2761 tctgcacagt gagcacaatt taatcctcct agtataaatt cttttttaat tgaattattt
     2821 gcttccatat atattcactc ctctcattta tcatttataa gtctctctaa tatgatttaa
     2881 tccacaatca aatatctgac ttatatgact atcatctaat gagtaataca ctgtttttcc
     2941 ttctcttcta aactttacta atctagcctg ctttaatact ctaagttgat gagatattgc
     3001 agaatgagtc atatttaata aactcgctat atcacataca cacatttcat ttgcaaataa
     3061 tgcatataat atctttactc tagtagtatc cccaaatact ttaaataatt ctgctaaatc
     3121 atataacatt tcttcatctg gcatagttga tttagcctct gttactattt cttcatgtac
     3181 tatattacaa ttacaatcat ttatctcttc tctcattttt aatcctccat tattttaata
     3241 tatgaataac tgttcatatg ttcattatat gttatcattt ctaaatttgc aacaataatt
     3301 tttattaatt taatataaat agaatgcaca tattataaac ttttttaata tatatattat
     3361 tagaataagt ttttgtaaac ttttttatag ttttctagga ggtgcaagtt taatatgagt
     3421 aacaagaaaa aaaaggattt agatactagt tacatgcctt acttagaccc agcgaactat
     3481 gcagattata ttactaatga agaaattccc caagatgaat tgagaaatga ccctcaaatt
     3541 tctaatggtt atacaaaaat acctaataac cagaacataa ataacctata tcctaataat
     3601 gtaaatcatt atcctaaaag tagtaattct ccaaacaact tgcctaacac aaataataca
     3661 tccggaaata tgaattacaa tacatctgct acaaataata tgcctaataa catgaataat
     3721 aatacgggta tgcctaataa tatgtctaac aatatgaata gtaatatggg tatgcctaac
     3781 aatatgccta ataacatgaa tagtaatatg ggtatgccta acaatatgcc taataatatg
     3841 aatagtaaca tgggcattcc taacaatgtt cctaataaca tgaatagtaa tatgggtatg
     3901 cctaacaata tgtctaataa tatgaatagt aatatgggta tgcctaacaa tatgtctggc
     3961 aatatgaata acaatatgta tggtaataca ggtatgccta ataacatgaa cagtaatatg
     4021 gggtataata tgaacaatac tatgggtatt cctaacttct catgtaatca aaatatgcct
     4081 cctaatgtac ttatgatgat accaggagtt atatgtcata atacaatgca aggtatgcct
     4141 gtagttatgc ctagtactat gcctcctaat atatatccta ctccttatgg ttctagtaat
     4201 atgtctatac aaggtatacc acaggctact aatattgagg aatttgatga ggaagaaatg
     4261 taatatatat ttttttaagt aagttgtata aaacattaat aattgaccat aataataaat
     4321 gtattcctca taatattccc ccaatattat tataggtata gaaagttgac tgaatatgcg
     4381 cttattcggt caacagatat taacataatc taataagata aacatagaaa aagtcagata
     4441 aaatttaatt ttatctgact ttttcattta aaaatgctct cttgtattgg tgggtcagaa
     4501 tagtatgata ctcgtatttg agactaatat tggcaaacaa cataaagaaa aatataaatt
     4561 ttttataata aactgtataa aattttaata attgaccaca ataataaatg tattcctcat
     4621 aatattcccc caatattata gaaataaatt taagcgagat taaattcagt taatctcgct
     4681 taaataatgt ctaaatttat tattccaaaa aatcatcaaa atctaatgaa cctgattctg
     4741 caaaatcata taatgcattt tgctcaaaga aaaatttctc ttcaaattta atatagtcgc
     4801 tataattaga ttgacttatt tcattgatat aattgcaatc attttcgtta taagtaccaa
     4861 taaaaaactt atttcccatt aagtaatata tagctttcat atcattattt attatatata
     4921 cattttcttt tacatggtca ttattaaaat taaatctagt aaagaatata ccttcattca
     4981 tattactttt taacagaaat ggattttctc tataaaatac tcctgcaaat tcttcaggtg
     5041 aatttacact gtaaacactt acaaattcac ttttagaaat ctcatcaaat acctcaaaat
     5101 catacatagg ttctttatct gtcactaata gagaattaat tctaaaacca gtttcataac
     5161 acttacttat gcaaaaagca attttacata tataatatcc atcattatct tcatataaag
     5221 cttcacttat atatctgcct tgacctttat cacttaccac gtttttaagt aatgttccat
     5281 ttatattagt aatgtgcata tttgcaatct cgtcacttcc agaaaagtac tttaaacttt
     5341 ctctatccca tgctataaat ctcatggtca tatagtttat aaattcaact tcctcgtcaa
     5401 ctcttttaca tattttattg tataattttt ctatagttaa atctgtttta tagtttttta
     5461 taatatcaat atactcacat ttatctcctg gcaatagctt atcataataa agattcttat
     5521 taccaaaata agatactaaa aataaagatt catccttcgt taattcaact ctatctgaac
     5581 caaatcctcc catcaatctt tcttcttcca tataagcttc ttcttgtgtt gggttattta
     5641 aactcacata atccgctaat cctaatccct ctgcatctaa taaaaaatat tgacaaaagt
     5701 gattgttttc atcatctatc caatttatta taagacctaa actgcccata agtctagaat
     5761 ttgtagcctt tgcaaaatta aaattcatat agtcacatcc tttaaactct aaaatacttg
     5821 ttttttaata tataaattat tatacatata ttttaacaac ttagatacaa tttataatat
     5881 aatataagga aatgattaat tgaggtggtc ttatgaaaaa tatagcagct ttttttgata
     5941 tagatggtac tctttataga gactctctta tggttgaaca ttttaaaaaa ttaattaaat
     6001 acgatataat tgaccaaaaa aattggtata aacatgctag agacactttt atggattggg
     6061 ataaaagaca aggtaattat gatgattatc tattagaaat atgtgacctt tatgttgatt
     6121 cactaaaagg attagataaa acctgtatca actttacaag tgaccaagta attaaattaa
     6181 aagcagatag agtttataaa tacacacgtt caaggattga gtggcatcta aatcaaggac
     6241 acatagttat atttatatct ggtagccctg gatttcttgt tgaaaaaatg gccaaaaaat
     6301 ataatgtaac cgatttttta ggaagtgatt atgtctttga aaacaatata tttacaggca
     6361 cagttattcc aatgtgggat tctataagta agaataatgc tataaacgat tttgttgtaa
     6421 agtatgattt agatttatca aactcgtatg cctatggaga tactaatggt gatataaata
     6481 tgctaaaaag agttggaaat ccaatagcaa taaatccaac aaaggaactt ttatctcaaa
     6541 tatcacttga tgaggaaatt tctaaaaatg ttgagataat agtagaaaga aaagatatta
     6601 tttattctct atctacagat gtaaatatag tggatattta atttatacta tctattttaa
     6661 aaacttatat tatatctaat atttatatct aacatataaa agctttgact aaaaatcaaa
     6721 gcttttatat gttgcactaa gaatatatta aatttagctt tatgctttaa tgtatattca
     6781 tatatatcat ttacacttat ctaaactaca atttcattaa aattaaatat aaaaatcaac
     6841 tattattttt taaatagcat ctttcttaat ctattattgt aataacctat aaaagaactg
     6901 taaacatagt catatactat aaaggctatc tcaaatgaac ctataataaa tatgtttatt
     6961 ggaatataaa ctattgttct taataaaaaa tacaatataa gtatcattat atttgcaaat
     7021 actaatttca taaaatactc aatatatata gacctatctt tttctataag atattttaca
     7081 ataccatata ttccaaaggt aagtgaatat acaatccatt gagacttact tgccatcact
     7141 ataaatccta aaactataga acctatataa aaagcaattc cactcttaaa gccccattcc
     7201 attattacaa ttgatactaa aagtgatgct aaccccataa taaacaatgt atttacagga
     7261 ataatatttg taagagtcaa caatatcaca tttaatgcta ataaaactcc tccataagca
     7321 acttttatac tcatatttat catcctaaca acaagttatt aaatctccac caaaacattc
     7381 acaacatgaa tctaaacacc aaagcttagc acaattttca caacaatcgt cacctagaca
     7441 acaacagcaa tcatcacagc aacagcaaca acatccatct aaatcattac gtcttcttct
     7501 attataattg tcagccctat tgctataatc atttctatat tgtgtataac ttctaagtgc
     7561 atcactatat tcactatttt caggctccat aaattttgct ctttttaaga agtcttcacc
     7621 ttcttcataa tatcctatgt tcattgcaga aaagccagtt aaataatacc actctgcaca
     7681 tttgtcagtc aaagttttta aaaaatcata tgctttttta aattcactat tttctatgaa
     7741 tgctcttgct ctcataaaat tttcattatt ataagtatta ctgtacattc tttccacctc
     7801 tactttctaa tatattaata tatcttaaat aaacaccaga ataaattata ttttctatta
     7861 ttccaacatt tttatttaga tttagattat caattcccct agctaaaaac cctaatgatg
     7921 ttactattat tttttttact ttttctttta actcatcatt tttatcaata tactctataa
     7981 atggattata tcttcctttc ttaaaatctt catctaaatc ttcataggca tctagtagat
     8041 atatatactt acctatatta aaacctatca ttcttaagtt tctctcattt tcatcttttt
     8101 tatagacaaa tacttcaccc attatatttc caaatatact agatacttca tctatattta
     8161 tatttttatc tttttctaac ttatttaatt catttagttg ttgttttata tactctgctt
     8221 tttcaggata cttttcatat gctaatttta atttgttttt atatatataa tatgccaatt
     8281 tatcttttat gcgtttatca tccattaaat tatcttccaa tttataatac gttaaaagaa
     8341 tattcatact tgctgcatat tctgtaatat catttgtaat tacctttttc tttttaaatg
     8401 gatttactat acacacttcc tctgtactta tagactctgg gttatatact gctgttaata
     8461 aaacaattaa aaatgttata tcataattca aagaaaacct agagatttct ccatgatttc
     8521 tttttaaata tttacatagt ccacaatagt aagctttgta ataatcgtat tctctaaatg
     8581 ttaaatccat tttgtttatt ttaacatatc caaacataaa tactcctttt tgtactattt
     8641 tttatttaga ttatatatag tataacactt tttatcaaga ttatataatt tacagtattc
     8701 taagaaactt aaaaaattcc ttctaatcat tgtatcttaa atttaaaata attagaaggt
     8761 tgggttaaaa ataatattaa attatatact tatattaact catacaagtt agttgtgctt
     8821 atatcaattt aagttttaaa taattggtat atatttaaaa ctacctacag tatccttcaa
     8881 ttcttttttt gctttattta taaccaattc atcaagcatt acatcattta ttgaacatga
     8941 tatagattta gatgccaata acattgcttt aaatcccata tcagaacctg cactggcaca
     9001 agattgccat gtatgggcag aaataccaat aggccaaact gaacaagaac actgtgctgt
     9061 aggaattatg taactaacat ctccaatatc caatgataat gatatacagt tattatgagt
     9121 aaatgtatca tccgtaactt catcatgcag attcatctta gtaatatcct tatttccact
     9181 taatacacta gaaacactac ttcttttatc tatagacact gtctcagcta atttatctgc
     9241 aaaatagtag tcatcttcat tatagttttg tacacctact aatttcatat tttgtgatag
     9301 aatatctgtt aatattgtat ttggaatata gtcatatact ccacttataa tgtcatattc
     9361 cataatagta ccagtcatta atgctgctcc ttttgctata tcaacaatcc taccaaatac
     9421 atctcttaca tgctccattt taactcctct tatgtaatac cacacttctg caaaatcagg
     9481 aactatattt ggcatttttc caccattagt agttacatag tgtactctac aattatcaaa
     9541 tatatgttct ctcaaataat tacaacctat attcataagt tctactgcat caagtgcact
     9601 tcttccgtta taaggagctt gtgctgcatg ggcactaatc ccattatatc taaatttcac
     9661 agagtaatta gcaagtgttg ggattcttat aggagtattt atatcaaatg gatgccaact
     9721 aaaagcacaa tcaattccat caaaaaaacc tttttttatc atcaatactt ttccagataa
     9781 atcttcctct gctggacatc catagtattt tatagtccct ttcagctttt ttagtttgat
     9841 taattccttt actgcaatta cagctcctat tgaacctgta cccagtaagt tatgtccaca
     9901 tccatgacct gcaccaccat ctacaatagg ttctttaata gtggatactt tttgagacaa
     9961 actatctaaa gcatcatatt ctcccaaaac tgctataact ggtctacctt ctccatagct
    10021 agccgcaaat gctgtatcca taccttctaa ttcatctata ctaaaaccat atttttttaa
    10081 gtattctttt tgtaaatttg aagcataata ttctttatac tgaagttctg gatttttcca
    10141 tatcttatct gatatatctc ttaattctcc ctgtatctca tctatatatt ccatcaaaaa
    10201 ctttttattt aaattctcca tattaattat ataaacctcc ctgtttctaa taacttatcg
    10261 attatcagcc aaaatgacta attttaactg atttaaaaat attttctatc atacaaccat
    10321 tttatacaat aaaacctctt gacttgtaag tttcatttta tcaagtaaga ggttttcaag
    10381 ttttataaaa tttttagcat gttaaccaat caagcatatt aacccataat ttattatagt
    10441 atttccaatc agtaaattct tttggacccc agtgaggtgc acagtcagaa ctaaagatag
    10501 cagattttcc ttttccaaac tcgccaacag ctacaaatgg gtctcctcca atagttgcta
    10561 aaacaggaca attatctcta gctactgttt tgttatatcc taaaaacttt ggccattctg
    10621 ttggtatacc ttttaatact ggatgtttag agtcaataac ttctggaatt attcctgctg
    10681 gtttttcaac tctatcatct ttatctatca ttgttattgg taatacatct ttgatagcag
    10741 tttctccgaa tctagctttt gcatctattc ctgtaaatga catatatcca cctaccatga
    10801 ttaacgcccc accattattt acatactctt tgattagttc aagtctatct ggattactat
    10861 tgcaatctat aaaagtacta ttagataata agaacgtatt agaacctata tcagataata
    10921 ttatacagtc atactcattt aactcttcta atttatacgg aaaactatct gctgctacat
    10981 gtgcaggcat atgaactgtc tcatatcctc cagattctat tgcttctttc aaccacttta
    11041 cagcttcttc ataatgagtt gtaacaaacg tatcaaatcc ttttatatga gttatatttt
    11101 ttacccaaga ttcccctact attaatactt ttttcattta tatattcctc ctacttttat
    11161 aagtttaata ttagacttgc atacataagt atactatcta aatactcctt aatttcaaca
    11221 tattcatcaa ttgaatgaca ttgttctatt gaacctggcc ctaatataag tgttggtatt
    11281 tcagctaaat ttttcattaa tctcgcatca ttgccagctg ttgaaccttt tatctctaat
    11341 tttttcccta gagtttcttc tagtatttta tgtgcattca ctacaaaatc agaatccttg
    11401 tccatctcaa aacctaaccc tgattgatat atgtttatat ttggtttatt atctttaagc
    11461 cataaatctc cattagccct tgtcattatg acatttgtta catcatttac aacttgttca
    11521 taactcatta tatttggcaa gaagtgtaca cataaattaa atacacactt atctggcaca
    11581 gtagaacctg ctgttccacc atttattaca cctaaattta ttgtaggtga aggtaatagt
    11641 ggatgtttat atatcataag ccagttatgt tccaactctt ttatatcttg aagcaacaac
    11701 atagccttct caattgcatt tacaccctcc cacttactac cacagtgaag agacacacct
    11761 ttaacctcaa cttcaaagaa tacaaatccc atatgtgcaa ctattaagtt ttgttctgat
    11821 ggttcacaaa ttatgcaaca atctccatca ataccattca taactgcatt tatggtacca
    11881 tttccgccac cttcttcatc tactactgac attattttta cattaccagg tacatttaga
    11941 ccactatctt ttataagctt tacagctaat attgatgcta taagtccaga tttcatatca
    12001 gcagttccaa gtccataaag ttttccatta tcttctgtag cacgatatgg attatatttc
    12061 cacttactta tatcacctgg tggcatagtg tctacatgac cattaaatac aattgtctta
    12121 ccaggtaaat catcactata cttacaaatt aaattatatc tatcttgata attgtgacct
    12181 agatttcctt ctttatatat attttttgct tttttaatta attcttcgct catttcttgt
    12241 ctatctacac taaaacctat gtaatttgca agtttttcaa tatattcctg tccttctttt
    12301 tcaaatccac ctagtattcc atgaccaaca tcttcagtct ttatacttac aagttctttt
    12361 aaataatcta tgtattcttg ttttttacta tttaatgtat ctaagagaat ttttttcata
    12421 tttcctccta agatttattt ttacatttaa aatttcaagt ctaatctcat ctatttaagc
    12481 catagtaaac tttttcactt agcatattcc cattcaatgt ataaagatgt atgcaaatta
    12541 aagtataaaa atatatacaa aaacttaata atttttatag tttataagct aaaatttaat
    12601 tactatttag atttagctga tttttttaat tttatattat ttatccatat agcaaataac
    12661 attaccaaac cttttataat catttgatta tatgaagata cacttaataa gtttaatcca
    12721 tttcctaata ttcctattat tccgacacct atacatgtac ctactataga accagaacca
    12781 cccatcatat tagtaccacc aagcacaacg gctgctatag catccaattc tgctccatct
    12841 ccaacagttg gtgaacctga tgccaaacgt gatgatagta ttatcccagc taatgctgtt
    12901 agtagtccag ctagtgcgta tacttttact ataactttat ttacttttac tccacataat
    12961 ctagcggcct cttcatttcc acctactgca tgtacatgac gtccaaaatt agttcttttt
    13021 aatagaaaat gtcctgctat tacaactata atcataatta taactggtat tggtattggt
    13081 ccaatatatc ctctaccgag catcttaaat gcatctgtat ttactgatat aggtcttcct
    13141 cctgtaaata caaagcttgc cccacttatt atcgttaaca tggctaaagt tacaatgaat
    13201 ggaggtagtt tttgtgttga aataattgca ccttttatga ctcctaaaat tgtccctatt
    13261 gctaatgcaa gtatcatacc tacccatata ggtgcattat aattcactac tgttgtagcc
    13321 caaaatgtac ctactaaagc aattactgct cctacactta aatctattcc tcctgtaatt
    13381 attacaaatg tcatacctac tgcaagtatt gcatttattg atatttgtct tccaacatta
    13441 attaaattat ctaaagttaa aaagttagat gaagctacac tcattattat aaaaattatt
    13501 acaagtccta cagctgcact cccttggtct ctaaatacac ttgttaattt tgtcaataat
    13561 ggagaagatt tttcttcttt atgcttagtt attgaactca ttaattttac ctccgaaagc
    13621 caattttaat acattatttt caaccatttc ttcaccttct aaaaatccac ttatatgacc
    13681 ttctctcata accattattc tgtcactcat tgatattaat tctggtattt cagaagatat
    13741 cattattata cttacaccac tctccactaa atctcccatt agattgtata tctctgcctt
    13801 agcatttaca tctatacctc tggttggttc atccataatc aatatatctg gttgtaataa
    13861 aagccatctt gctattatag ctttttgttg gtttccacca cttaaacttt gtataacttg
    13921 attcatatgt ggtgttttta tctttaaagc ttctatgtat ttttctaaaa tctctttttc
    13981 cttttttcta tctataattg atttaaactt agatatcttt tttaacccag ccatagttgt
    14041 attttctttg acactcattt gtaaaaacaa tccagtacct tttctatctt ctgttattaa
    14101 tcctagtcca ttttctattg catctgatgg agttttaaac tttacttctt cattgtttaa
    14161 atatattttt ccagaatcat atgttctaag tccaaatata gcttctacta tttcacttct
    14221 cttacttcca actagtccac ctattcccaa tatttcacct tttcttatat taaagctaat
    14281 atcattaaaa acattgttct tagttagatt ttccactcgc ataatttctt cttttatttc
    14341 tacttttctt tttgggaaaa attggtctag ctttctatct atcattaaat taactaattc
    14401 ttcctcagac gatacatctt tggtatttaa agtcgaaatc gttttcccat ctcgcattat
    14461 agttattctg tcacaaatct caaagagttc ttccatccta tgggatatat atattatact
    14521 aactccactc tcttttaaat catttataag tctaaaaagt atttcagtct cttttttact
    14581 tattgaagaa gtaggttcat ccattattaa tactttagtt tctgatgata aaactcttgc
    14641 tatttctacc atttgttttt gagctatact aagctctttg actaaactat ttggatttat
    14701 atctattccc atattatcga aaatcttttt agcttcttca ttcatttttt tgtcattggt
    14761 taagactttg ccatttattt cttttcccat aaaaatattt tgtgctactg ttaattcgtc
    14821 aaaaactgtt agttcttgat atatggcatt tatacctatc tttttagaga tatctgggga
    14881 tatattctct atttctttgc cttcaaaaat aaattttcca gaagttttcg aatgtgcccc
    14941 agttaaaatc tttatcattg ttgatttacc agcaccattt tcacccagta gtgcatgaac
    15001 ttcgccatga aataactcaa aattaacatt atctaatgct ctgacaccag gaaattcttt
    15061 agcaatatta cttaacttca gaattatatt actcataatg acactccttt ttaaatagga
    15121 tttctttcaa ataatctcat tttattttac taattcttaa agtcttttat attttcctta
    15181 tcataaagta aagctggtac tggtatattc ttttctacct tttccccttt tatagctttt
    15241 actactgctt ctacagctaa acgtcccatc tctttaggtt gttgttgaat catagcaaga
    15301 atagttccat ctgttattcc acttgatgct tcttcagtta agtcaaatcc tacaatctta
    15361 acatccttat tttttgcaga ttcaagtgca gctttagctc ctagtacact attttcagct
    15421 gttgcatata ttatatctac atctggatta gattgaatta agttttctac tgtattaaga
    15481 gattcttctc tgtcatatcc tggttgtgaa tttaatatct taacatttgc tgaacctttt
    15541 aatgcatctt taaatccatc tattctctgc atttgtattt gagattttaa atctgtcact
    15601 attgctattt ctgactttcc acctaacttt tcatctatgt attttttagt gtattcccct
    15661 aattttttac cagcatcata actaactgtt cctacataac ttgtaatatt ttcagtattt
    15721 aatacagcat ctactgtaac tactggcatt ttttcaaatg ctccttctac tgctggtact
    15781 atagccgaat tatcagttcc aagtactatc attccatcta cattctgttg tataaaatct
    15841 tctattgctg atgtttgctt agctacatct tggtctgggt catttactat aactttcaca
    15901 cctaattctt tagctttttc ttttattccc tcatcaatat ctataaaaaa ttgatgttga
    15961 agagttattg tagatactcc aatagttaaa tctttcttgt ctgaaccacc tttattatct
    16021 gatttacttg gtccttccat tgaacaccct gttaacattc ctattgctag tattccagca
    16081 attcctaaag ataaaatttt tctcactctc ataattaacc tccattttta atagttttaa
    16141 atttgaacaa attagatttt tatttattat ttaaatactt ttcaacatct tctttatagg
    16201 gcataccaga ttgtgcacct agtttagtta cagaaagacc tgatactact actgcatatt
    16261 ctactgctgt atataaattt ttcccttctg ttatagccac tgccaatgct ccattaaatg
    16321 tatctcctgc tcctgtggta tcttgaacat caacacttat acaagataca tgcttgattt
    16381 cctcaccatc ataaaatcta gctccatttt ttccttctgt tattacaagt ttatttggat
    16441 atttttttaa tacttcttct atcccttcat ttgtgtcaaa aactatctta tattcatgtt
    16501 catttggtgt taagtaagtt actttttcta ttatatcttc atttagtttt actgctggtg
    16561 ctggatttaa taaaacccta atcctatttt caaaacagaa attcactaca taatttattg
    16621 tttttaatgg aatctctaat tgtagcaata ctatatctgc attctttatc tcttcttcat
    16681 tctttttaat taattctata tctactagct cattagtccc tggtacaaca acaatactat
    16741 tatcattttc acataatgtt ataacagcaa caccagaact tttgtgatta tttctggata
    16801 ctaagctata atcaacttta ttcgttctaa aatttcttat taactcctct cctaagccat
    16861 cttcccctat gcatgatatc atttttacat ttgctcctag tttagaagct gagatagctt
    16921 gattagcccc ttttcctcca ggtgatgtaa aaaaactatt acctaatact gtttctcctt
    16981 tttctggtct tttatctaca gaacatacca tgtccatgtt tacacttcct atgacaacta
    17041 tatttcccat tacattactc ctttacatat ctatttatgt ctctcgtgct gttattcact
    17101 ttcttttgtt gaatctctta tgaccaattc tggtttgaaa atcaattcat caaacgattc
    17161 ttttttatct cctcttaaac aatttatcaa tatttcactg gctttatatc ctatatcata
    17221 agacggctgt tttactgtgg taagagaagg gcttattaga cttgatgttt gaatatcatc
    17281 aaatcccacg acacttatat catttggtat acttaatcct ctttctttaa gacctcttat
    17341 gacacctatt gcaatcaaat catttgcaca aaatattgca tcaaaatcta tcttttctaa
    17401 attttttata aattcttgtc cccattcaat cttatatttt ccttcaaata cataagcagg
    17461 attgtagctt aaattacact cgattagagc ctttttatat ccttctaatc tatctactgc
    17521 tatttcattt tgaagtggac cacttaggta tattattttc ttatttccaa tatctaataa
    17581 atgttttgta gctatatagg ccccttcaaa gttatttatt ttaacaattc cttttaaggt
    17641 ttctttgttc atacttatct tcttatcaat caagacagtt ggtattttta tatctttata
    17701 atttgcttct ttccagtttg tatttgaaga tgctgcaaat ataattccat ctatcatcct
    17761 ctctgatagt gttcttattc cttcatattc ttttttagca tcttcatctg tattacataa
    17821 aattatattg taaccttctt cattagcttt gtcttctaca cctcttgata catctgtaaa
    17881 gaatggattt cgtatatctg gtataagaag ccctatagta ttagtttttt tagttactaa
    17941 actttgtgct aatttatttg gtacataatt tttttccttc attattttca aaattttttc
    18001 tcttgttgcc tgacttatat tttcatcttt attattaatg acttttgaca cagttgtttt
    18061 agaaacacca gccaattcgc caatttcttt tatagttata ctcaagaagt tcacccccaa
    18121 aatttaggaa accgttttac ttaaccggtt acctagatac tatcacatct tttttccatt
    18181 tgcaaccatt ttatataaat tttgatattt ttcccttttt tcaaaatatt gcaacttata
    18241 attcaatttt aatatttaaa ttaatctgca aaaaaaaatt aatatacttt tataattatc
    18301 taaaagtata ttaattaatt taagcagtat ttattaaagt tcaaatcctt gctctataat
    18361 cattttttta tcattttcaa tcccacttcc aacagttgtc aaataatcac caacaattgc
    18421 agaatttact cccccattaa atccaacttt gtcatatccc tttaatgata gccttccacc
    18481 agcatacctt atgtacacat taggaattat aaacctaaat actgctgttg tttttagtat
    18541 ttccattggt tctaatacat cataattttc cattggtgta ttcttaatag gatttaaaat
    18601 gtttattgga aatgatttta catttaattc tcgtatttca aaagccattt ttagtctctg
    18661 ttcttcactc tcattcattc caattatacc acctacacat acatctagcc cagctttttt
    18721 agcatctttt atagttttta tcctatcttt atatgtgtgt gttgtacata tattatgaaa
    18781 attatctcta cacgtctcaa cattgtggtg atatgtagtc acacctgctg acttcaacat
    18841 tttagcttgc tcataatcta taatacctaa agatgcacat aattttaaat tagtattttc
    18901 cctcaatcct tcatagatat ttaaaatatt attaaactcc tttcctgaca tacctttacc
    18961 acttgtaacc aatgagaatc tatgcactcc tttactctcc atctctttag ctctatttaa
    19021 gatttcattg taatctaata atgaatattc ttctacacca gttttataat gagatgattg
    19081 agcacaaaat ttacagtctt cactacattt gccagattta gcattcatta tcgtacataa
    19141 atctgctttt cttcccataa aatattctct tatttcattt gcagatttta atagaatatc
    19201 aaaatcgttt ttattatttg tatcaaggct tattaaatta agagcttctt cataacttat
    19261 ttctttttct ctcaatactt tattttttaa ttttattatg tatttttcca tatatcctcc
    19321 tatttgtcaa cttaatattt ttatgtagtt tacttattat atatagtatt gcatatataa
    19381 catttattgg tcaactttat tttagacaca agtttactat acacaaaaaa atactgcttc
    19441 aaaataatat tagtaaatgg attatacata ttattattaa aacagtattt tttattagaa
    19501 tataattctt actggtttat aaaactatcc ttgtcttaac tccatccaag ctttccattg
    19561 taataacctc taagccttca tattctctaa atagccattt taaatccttc atggcttgat
    19621 taacctgact ttttgttaga ttatttataa aatattcttt tctagtttcc tcatactctt
    19681 tgctagtcaa aaagtttctt agaaaattaa acgcaaatac acttggaata ttgtttacat
    19741 tagccttctt gctgttttcc ttctcatagt aaattttcat aaatgcatac tcctttgata
    19801 cttttagatt tctcttacaa taattatatg catttaagtg ctactattct tataatttta
    19861 ttaattcata ttctttactt cccataccca tttcacatgc atagtctatt attctagttc
    19921 catttacatg aggatgttca tgtttgaaaa catcatgacc catttcctta caaatcaaat
    19981 catagcatgc ttgttcaata gctacagggt ctgttgatgc taatattcct atatcatggg
    20041 caattggtct acctgaccaa ggtacacaat cacaaagtgg agttacattc ataacaaaag
    20101 ttatataccc taccttgtct tttttatttg atactgctcc ataagcgtat tctgccatct
    20161 tttcgacaaa cacatctgag tcactttccc attgtatagt tactgctcta gttggacaga
    20221 cagttggaca ttctccacat ccataacata catcagaatc tataactgct tttttatcca
    20281 ctatacttat agcttttgta ggacaagaat taacacactt tccacaacct acacacttct
    20341 tttcctttac tactggagta acatcagaat gttgcatttg ttttcctgct gcacttgcac
    20401 atcccatagc taagtttttt aatgctcctc caaaacctgc tgcttcatga cctttaaaat
    20461 gactcattac tatcattgca gaagaattat aaatttctcc acctattttt acactttcaa
    20521 aatgtttttt gtctattttt acattctcat aatttctact atataatcca tctgcaatta
    20581 ttactggtgc atttacaact gcataagcaa atccattttc tatagcagtt gtaagatggt
    20641 ctacagaatt tgttctactc cctgtataca gtgtatttgt atctgttaaa aatggctttc
    20701 cttcgcagtc tttaacctta tcaaccactt gtctaactgc cacaggactc atatatgtag
    20761 tatttccctt ttctccaaag tgaagtttaa ttgcaacttg gtcattcttt tctattaaat
    20821 ctttgaatcc tgcttcatca aataatcttc ttacattgtt tggtacgttt ttattttgcg
    20881 aatttgagta taaatcacaa aaatatactt ttgacttttc catatctaca cccctttaaa
    20941 atatttactt atactaattt tatcactttt aaagtaattt ttagtatatt ttaacataat
    21001 taaatattct tatatttatt tgatacaaat aatattattt gtattatttt ctacacttaa
    21061 ttctatatat taaaaatcaa aaagacttga aacttaagtc caagtctttt tgatttttag
    21121 gtgtagtttt taacaacata agattacttt accttgtgat aaaggtatta ttaaactggt
    21181 tttttattaa ataaaataac agctgctagt aaacatacta cagaaataag aactgttata
    21241 ataattggaa taataaattc tggcattgcc actttccctg aaatcaagtc tatatttttt
    21301 gacatcaaga aattaggagt ataaggtgca atctgtttag gaatagaaat taaactcaat
    21361 aaaagagttg ccaccaatac aagtaaaaca ctggcaaatg ctggtctaaa tagtacacac
    21421 ccaagaatta atacactaat atacataatt cctataagcc ataaattaaa tgctgaaaat
    21481 ataatatgat taagatttgc agttggccaa aaatatattg tatagccata agcaaataaa
    21541 aaactcaacc aaaaaccaat tgtcataata atcaccgtaa cagagaattt agacaatact
    21601 atagatgaac gtgataaacc tttagtcagc ataataacaa gtgtcccttt agaatattca
    21661 tttgataaaa tattacaaaa aataattaga gttaaactca taccaagacc agagatattg
    21721 ctaaaaaatt gtgtccaaga atctaaagca gttggtgcag cagtaacttt taaagtcggt
    21781 gcaaaatgag caatcaagtc tggcataaat tttgcagaaa taggactact tataccaaat
    21841 attagaaata aaataattaa actaaataat ttataatttc ttaaattctc tttaaattct
    21901 ttttttgtaa atgctatgta ttctctcact ttactacctc caaaaataaa ttttcaagtg
    21961 ataattctat tagttccatc tttactggac aaagattcat ttctgcgagt gtagatataa
    22021 ctgttttttg tattgctttt atatctttac catgcaaaac aatttccata ttagtttctt
    22081 ctgaatcttt tagtaaggat ttaataccat cactagactt aaacttttga atttcatcat
    22141 tacttgcaaa ctccagtaaa agtctatcct ttccgtgcat actttttatt tctgaaagtg
    22201 taccactaag ggcaatgctt cctttgttta atatagcaac atggtcacag attctttcaa
    22261 catctgatag tatatgtgtt gaaaatatta cagttgttga gtctttgatt ttaagcataa
    22321 tatctaatat ttcttttcga ccaacagggt caagtgcact tgttggttca tcacaaataa
    22381 gtaatttggg gcgtgaaagc aaagcttgag ctataccaag tcgttgcttc attccacgag
    22441 aaaacccacc tattcgtctc ttttcatttc caagacccac aagtgataac aactcttcac
    22501 tccttatctg tatttctttt tttgaaagtc ccgtaatttc accacaaaga gaaagatatt
    22561 caagtggtct catatagttg taaaactctg gaacatcagg cagataacct acatgacgat
    22621 tagaacttgt tttcccatag gtcactttct caccacaaac atctatagtt ccactatctg
    22681 gtttaagtaa cccaagtacc attttcattg ttgtagtctt acctgcacca ttcttaccta
    22741 caaacccaaa gactgaccct tctggtacag taaaatttaa attatctatt atctttcgat
    22801 taccaaaacc ttttgataat ccatttattg agacaatatt catcattctt cacctcttcc
    22861 tattgtaaaa tacaaaattg gtcctattag actaataaac agtactacaa taatccatat
    22921 tgccttattt ccaattttat aatttggatg tctcaataca tgaacaagtg cagtaattat
    22981 aagtattaaa tctaaaataa taacaggaac caaaagtggt aagtattcca tcaaattttc
    23041 cataatctta ttcctccaat aaagataatt tttctataag aaatttgtat tctttgtttt
    23101 cacttaatac agaaaacatt ggattatttt taatagcagc aacaatacct gcttttacga
    23161 ttgtatcatc acgtgtaata cctgttccaa tttcagaaat ccatctgtca agtttattaa
    23221 aaaagtcatc cccatgtatt ataattgggt atatatcacg tgttgctaaa tctacatatt
    23281 ttcgtaatga tttaatagct ccttcattat caccatgtat taaattaatt tgtgcctgtg
    23341 ttaaatatga agaaagcata atagcaggag ataatgtatc aagctcaaat atatctgaaa
    23401 ttgctagaat acgtctttct atttccttca tttgtaattt atctgcattt tgcaaaatac
    23461 ccactaggtt ttggactaat gatataaggt tctgatatgc accaagttga aatgtttctc
    23521 tcgcttcatc catttgacca ttcatcattt gcccctgtgc aagaagtata gattcattta
    23581 tcatgggaaa attgctgttt tgcaataaat caatcacttg tatgggttga tttaacaata
    23641 tataacaagt tgcctccata ctttttgctt gacggcaaat atctatgtca ttacaagttt
    23701 cctgtatacg aataaatatt tctaatgctt cactaattaa tagttcacgc tttttttcat
    23761 ctacaatatc atagtgatta actattaaaa gacccatccg aaaaatcaaa ggaaaacatg
    23821 aataatattt ttttatgatt tctcttattt catccatgac ttcatcaaat ggtttaaccg
    23881 taaagtcttt gcacagttta ttatacagtt tattaatatc ttccttcatc atttgaggtt
    23941 cataacatat taactcatca acggtaatgt tgaaatatgt cgcgagttgc ggtaaaaaag
    24001 ttatgtcagg atagctttgt ccagtttccc atttagatac agaagcttta gaaacgccta
    24061 tataatttgc aagttcatct tgtgtaattc ctttttcttt acgctttaaa atcagcgttt
    24121 ttgcgatgtt taattcattc atgtcaatcc ctccttatgt ataattatat ccttaaacat
    24181 gtaaagctaa caagcgattt gaagttgact tttgacgaaa aaatcaacca cagagaaaca
    24241 aataaacaag tatataaaat atatatttaa atttatcttt aatctctttt gtaaaatatg
    24301 taaactttgt aaataaaatc catattatat aaaactaata gttaatctat ctaaatacag
    24361 tttttattta tcacgttttt aaatattttg tgtaaaacct cttttaatat aaacacctta
    24421 ttttcatttc tcatttcata ttctttttaa aatactgaat ataactcttc gattttccat
    24481 ttaattcttt tataaagtta ttattcgtat ttatttctct aattataaca ggcaactatc
    24541 atgtattggt atttgtctat tatataaaaa tctataaaag aaaagcagtc aatcatttag
    24601 attaactgct tgtacatata tcttattaaa ctgtataaac tataattttt attattctcc
    24661 attttcatcc caagtcataa atccccatcc actatctgga taagtatcaa tagacttttc
    24721 tctaatcata taaccaactt tataacactc ttttgttatt ttattagata gtatacatcc
    24781 ttctccattt atctaattcc attcaattaa cttttctttt caaccttaac ttttataaaa
    24841 tcatcttttt taataatttc atacttattt tctcttcaac atcatcaaat gagggtgtct
    24901 caaaattaac tttttacttc ataaggcact cttttttgta tgaagtaaaa tatattttca
    24961 tcatttcaac aataagtaag aggcttatct ctattttgag acagccccat ttgatggtgt
    25021 tgaacattta aataaggttg aactcgtttt atctctcttt ttaagatatc tgtaaatttg
    25081 cacataaagt ctcttcttct tttacaattc taatagtttt attaaagcca aagtatatat
    25141 ctccatattc tttcgtatat gatttattgg tactctttca tctactccat gaccaccagt
    25201 tttatacgct aagaacttag gtccaaacgc caccatatct ttaaaccaac ttgcatatgt
    25261 gccaccaccc atagcaattg tatatccatt ttcatctgtt acttcttcat aggcttctaa
    25321 taatttttta agtactaaat tactctcatc cacataatgt aagtctttat gagcaataac
    25381 ctctattttc caaccattac aaaaaacctt tttgaatcta tctattatat tgtttaaaac
    25441 tatattttta ccatacctta tatctaactc acatacgatt ttttcctcat ttacttttaa
    25501 tattcctaaa tttaaggtag tgtttttgaa tacctcatca tattgattaa tccctaatgc
    25561 atctccataa taatcactac aaaggtattt tgaaactaat tcgatatctt ctctatgctg
    25621 aaaatccata ttatcctcaa ctatctttat taccatttta gatatagcat tttctccctt
    25681 ttcaggatta cttgcatgta cagctactcc tttagtagtt aactctgaaa taattccatt
    25741 aataattttt gcagtacaat gagatggaac aacattttct ttagttcctc cccttactat
    25801 tatgtaaggt ttacttttgt caattttaat ttctttttct atccttatat gaacccttcc
    25861 tttttctcca ttaaccactg gatacatagc atctggagta aatcccatac ttggaatttc
    25921 ttcaccttta tctaagtaat atttgataca tttcatacct gtctcctcat ttgtaccaaa
    25981 aattaatctt attcttttat taattgtagg atacatatcc tctatgtttt tcaatacatg
    26041 tataagcata gatacagcac ccttattatc tattacacct cttccataaa taatattgtc
    26101 aattactgct cccgaaaatg ggtctacact ccacccctct cctggtggaa ctatatctaa
    26161 atgcattgga atccctatta attcctctcc ctctccaatt tctgcatatc caatataatt
    26221 atcaatattt ttaacactaa aacctaaact agcacagtaa tctaaagttt tatctaaaga
    26281 ttttttaggt cctataccaa aaggacattg tgaaataggt tcaccttgtt gtgaatcaat
    26341 ttttatcaat tcttttagaa attctatggt tctatttaaa ctattatcta cacttctgct
    26401 tatatccata aatcaaccta ctttctatct ttttaaagcc tgtccttaaa gaacaggctt
    26461 ttagcattaa aaattatttt tttgtaaata ctaattcaca aacttcatca tcatgagcac
    26521 atttttgagc aaaatctaat ttaacacctt gtgcgcagtc aactcttcca taatccccat
    26581 aacaagctaa tctacaaatt tcttttcttt cttctttagt taatccatat tctttccaag
    26641 cagcatctaa tggacagtga tggaatttta aaacgccatt ctcatctgta ttttctacta
    26701 attccatttc aaacactttt tgtcctttac tgttatataa catttctgcc atttttcctg
    26761 gagtatctgc tacgttatat ttttgacctc tcattttacc aaatccatat attgcttctt
    26821 tacatatatc atctggatta gctcctaatt tttcagcttc tttcataaga agatagaacc
    26881 atactgctct ttctcctgtt gctcctctaa ttccttctaa atattccttt tctgtataca
    26941 ttttttctga catttctttc tcctcctaaa attaaattct atattttaat cactgtgaat
    27001 tagaatattc caatgaatga ccctaccaat gctaatacaa ctaaaattac taataactta
    27061 gttattgtcc acttcttgta tttcattccc caatatacga agaatacaac accaagtggt
    27121 aataaacctg gagcaatcat atttaatgta tcttgaacaa caattgcttt accaccaata
    27181 tcaaatgata gcggagttga aagcttaact gtagttgcag ataatgctcc catcataaac
    27241 attcctagaa ttgaagcacc atcaattatc ttattgacaa taccacctga tagaattttc
    27301 ttaattgaat ctctacctaa agaatatcct aaatgaaata agtaataacc ttcgcaaaat
    27361 agtaatatac taaacattat tgggaaaaat gcacctataa cacttccttc tgctccaaat
    27421 gaagcaccta aagctaagaa tattgtttga aatgtaccaa aatctaaagt atctccaatt
    27481 ccagccaaag gtcccataag accattctta acacctgaaa taacttctcc tgggattttt
    27541 ccttcagtag ctttctcttc ttccatagct aaaactgtac cgtgaattaa tcctccccat
    27601 atagcttgag tattaaaaaa ttgtaagtgt ctctttaacg catctactaa atcttctttt
    27661 ctcttatata acttttctaa agccggagtg aaacttgcac aaactgctaa tgcttgcatt
    27721 ctttcaaaag agttggataa ttctgctgtc caccaccatc ttagatatgt ttttgttaca
    27781 tctttctttg taagtactct tgtcttatta acttcagttg gaccttgtcc atttttaact
    27841 tctttatttt ttatttcttg cattatactt cacctaccct tttgtcttct ttcataaagg
    27901 taattaaaag agctatacaa acaccaaata ttgcagctgc agtaattgat atttcaaaat
    27961 attgaaccat aaaaaatcct ataataaaca ttggtaaaaa tttattttta cctatagtaa
    28021 atattgttgt tgcaaaacct aatgcaggta atataccgcc cattactgtt aatccattca
    28081 ttaaccattg tggtataaca tctaataatg attgaactaa atctgctcca aaaaagttac
    28141 atacaaaaac tattggaaac cttaaaacaa acccaaatat taaagaataa agtgttgcac
    28201 atctccagat gccttttgta ttaccttctt cagcatactt atctgcctta tgaaccaata
    28261 cagcattacc agttcttctt aagttattaa ctaatactcc tataacacct aaaggtaccg
    28321 caatagatac tgcaacttct gcattaacac cagtttgaag tgctactggt attgcaataa
    28381 gagcagctag acatttatct gatggaatat ttccccctgc tgcgaccatt cctagataaa
    28441 ccatttctat acttgcacca acaatcatac cagttgttac atcaccccaa agtaatccaa
    28501 ggctaaaacc tataaataaa ggagacttta gtgttgagaa aagggtatac cctgcaagac
    28561 ctgctgcaaa ccaataccat aatcctgtta caattgccaa catcaaaaca gattccattt
    28621 ataattcctc ctaatttata ttttttattt aaatttcttt aacgcatttt catatgtcat
    28681 agatgattcc tctggtataa cttgtaggac tatttcaacc ccattttcat taagttcatt
    28741 aagttgttct acatcctcat ttcctaatga aacagctgta aacaccattt tctttcctgc
    28801 ttcatttgga atacctccta attgaatctt ttcatattta agtccagctt tataagcctt
    28861 atttgccata tcaacatttt taaatagaat aaatatattt ttatctccta aagtgttgtt
    28921 ttggaatgct tgtcccgcat cttctgctgt taaaataact acttctactc ctgatggtgc
    28981 tgccatttta tatatgtcag ccataaattc atcttgacct aagaagtcat caactattat
    29041 tattttttga gcttgagcga tttttaccca ttttgttata acttgcccat gaattaatct
    29101 gtggtctact cttactaatg ttatttttgc cattatattc acctcataat ttatattttt
    29161 ttaaattctt taattaaatc cacacaaagg ttattatttt ttttgacgat tctttctggt
    29221 aatgtatgta atttatcttc tttgcttaat tcttcatcta cgattaaaat tgtctgtaag
    29281 ctaagtccat ttattgcatc tattcctctt cttatagcaa cagctattgc aacacttgat
    29341 gtagatcctc catataaatc tgatattata attttcacat cttctttatc tactacttct
    29401 tcaacctctt tcataaatac agaaacatct ttttcagaac ttagtgataa aacttcatat
    29461 tgatttgaca atccaaagtt tttatttgtt tcttctacta agacttttcc ccaagttcca
    29521 tggcacagta tgattacctt atatttattc attgctaaat aaactctcct ttatagcgat
    29581 taatattttg gtttaattct acttcttctt catcaggtac catttgaatt gatacattaa
    29641 tattcttttt aataaattca tctattaaaa gaaactcttt tttattaatg tatacatttt
    29701 gagatatttt cttttttcct attcctgtag gtattcttcc aacaataagt tttttgatat
    29761 taatcccttc ttctatcatt ctatttgcag tatctaaatt tttaattaat acaaaaacgt
    29821 tatcatcagc ttgattatta taaaaatatc tccttgattc acttacagtt aatattgata
    29881 aatcaatatt ctttggtgct gccattttta tcaaaccttt agcaataaca tcattggttg
    29941 tttcatcatc tactactact atctttttta cacctagatg cttaacccat ttatatataa
    30001 cttgaccatg tattaatcta tcatcacatc tgcaatattt tactaaagac atttttaatc
    30061 aaactccttt ttctattaca ttgagctaag aactttgtta atattgttta taccagtttg
    30121 tcctgcttca atacatgctt ctgctaactc ttctaaactt tttccctctc tattaattaa
    30181 agcttcaagt agcattggaa gatttactcc agtaagtgca tgaaacttgc cacctttttt
    30241 aaggtttaaa cttgcaacat tacttggcga acctcctaat aggtcagcta atactaaaac
    30301 tccgtcacct tcatctaatt catttaactt ttctacaaaa tttttattta agtcttcaat
    30361 attatcattc ctatttagtt taattgctgc tgttctttct gctggtcctg caataagttc
    30421 acagcttttt ataatttctt cactaaaact cccatgagtc ataactatta ttcctatcac
    30481 caatttcacc tcacttttct tgtcactttt tatataagca attgccgtgc caacttatgt
    30541 gtatcaataa caaatggcta ttttacagga tttaatcaaa actttttatt caattttttt
    30601 taaaaactta ttatttcgat atgtatcaaa agattttttc aacctgtatc aaatctgtat
    30661 caaaaaaagt aaaaaaaaga tagccttttg gctattttta aattttgtat caacttttga
    30721 atttatctgt atcatattgt gattcaaata tatctaatat atttgcatat tccatatctg
    30781 aaataattat atcgaatgtc tcttctacta gcttaaaact ttcttttata actaaatata
    30841 attctttatt tctactaatt ctttcttcat atgatttata aactaaagac tcttttatta
    30901 ttactctttc aatcatacat gaacaatgaa atataaactt tactaaaagt tcatcttgaa
    30961 ttactaaaga atagtgttca ctaattctat taattacttc ttttaatata aaataagcct
    31021 tttccccatt taaaaaagtc aaagttttac taatattatc aattagtaat ctatcaaaat
    31081 atctaccacc attatctttt tcagcatatg taacttccat atctccataa tgtttcttta
    31141 tagattctat aatattaaaa tataatttct ctaatgtcat ttctggagtt agagcctttc
    31201 tagttgcttc tattacaata ggtgtactta ccatatctat tgcttttact tcttttcctg
    31261 tagcatcacg aataacttta gcaaatgtta aaatagaccc catatcagtt aaaattaaaa
    31321 ttccctttcc tttatcaatt ttattggcta tttctacagc ttgtactaaa atatcctgaa
    31381 ctctatcttc taatcccata ttaattgcca ctgcatggtt acaatccagt aaagtattgg
    31441 ctacattcac catgctagat gctgtagatt caccatgcat tatgactaat attgctatac
    31501 tctcctcatt taatcccaca tacgataaat aaagaaatgt tgctaaaaaa attgcttcat
    31561 cttctggaaa ctctactcca tacattaatg acatgttaga taatatttct tttgctatgt
    31621 tatattcata tgctctttcc ttcataattt catctttatt aggatgcttt actatatttc
    31681 caatctttat tctttcaatt aaattctgaa tatgtaaagc tatagctttt attaatttat
    31741 ctaatccttc tcttttgctc catttatcat gcttttgtaa aatatatttt acagtagttg
    31801 caattgaatc attaacaatg ttgttataag ctgtttgatt actactatta tatataaaag
    31861 tattcattaa attataggaa tacttctgta tgtctccatc tataacactt ctaatttgtg
    31921 actctttaat accttcttgt tgaagtttat tccaagtttc ttttatcatt ccataaaaat
    31981 ctaaatcata ttttttattt tctattaata aattttcact ttcaaaatcc ttttcttctg
    32041 gtaaaaaaat aatatcatct tttccaataa aactgaaatt ttgtacaaac tcctcttttc
    32101 tattatttcc atataatgat tctctaatat tatttggtag aagagagagc cttacatata
    32161 cacattccct tttataggtc atataatcta aaaaagctct tgcacaaatt aattgtatat
    32221 cgctttttaa ttggcctatg tttcccttac acttatataa aataaatgcc ttaataactt
    32281 cttttgaaac ctttaactta acaccaatcc taacactctc ttctttaaaa aaataacata
    32341 taagcttcat tctctcttga aagcctcttt cctctaaaga cggaatcttt atagttacag
    32401 gtattcttct taaaaacgtt tccaacattg ctgtttctgg gtcttctgta gtggctgcaa
    32461 ttatcataac tctacactcg tgtaccttat tagcctctcc taaccttcta tatatccctt
    32521 tatccataag cagaaatagc atttcttgtc cctctgcaga taatctatga acttcatcta
    32581 aaaacaatac tccattatta gcttcatgta caataccata cttatcttta tcagctccag
    32641 taaatgctcc ttttacatat ccaaaaagct gcgctaataa taattgctga ttatcagaat
    32701 aatcagcaca gttaaatatg ataaatggtg cattatcact atcttctttt atagttaaac
    32761 tatacttata catatattct gcaaatatag atttaccaac tcctgtaggt cctattatta
    32821 atgtactcaa ccccttaggt ggatatatta ctgctgcttt agcctgttct atttgggatc
    32881 ttaaacttcc atcacatcct actaaattaa aaatattgtt ctcttttttt atactctcta
    32941 aaatatactc atctttgata gaatcatttt cactatattc gtttatagag ttaatattat
    33001 caaatatccc tttatttagt ttctttccta aaatactctc aatactttct ttatataagt
    33061 actgaacagg cttacccttt atctttataa tgatactttc ttttattaat tgatttaatt
    33121 ccttactagc attacttcta tttataccta cttcagtagc tatatattct gcatcaaatc
    33181 caaatctaat gccattatta aagttcaagt gttcttctgt taaccttttc aagcaaatta
    33241 aaactttttc tttccttgat agcttctttt gtgtcatagt taacctcccc tataagcagt
    33301 aatactgtta aacatttatt attaagctta attctacaat attttaatat ttttgtctaa
    33361 ttatgtaact attgattttc tttcctattc gttttataat ataaattacg ttaccctcat
    33421 cttttataga ggaggaattt atatgaattt tttatcagat ttacacaccc actctatagt
    33481 cagtggtcat ggctatagca ctttactaga aaatattaat tattgtaaag aaaatggaat
    33541 taagatacta ggtacatctg aacatggtcc taagatgcct ggttctcccc acagatggta
    33601 ctttcacaac attcaaaata tcccaagaat cataaataac attataattt taagaggctg
    33661 tgaagcaaat atactagata ttaaaggaaa catagaccta gagccttttg taattcctcg
    33721 gttagattat cttatcttgt catttcacga agctgtgttt tctccaaata cattagagaa
    33781 taatactaaa gctttaataa atgcaataaa taagcatgat aatattgaaa tattaggaca
    33841 ccttggaaac ccaaactacc caatcgacta tgaattaatc attaaattag ctatagaaaa
    33901 gaatatctta atagaaataa ataattgttc tattaaaggc gtgtcaagaa atggtagctc
    33961 ggataactgt aagtatattg ctacactttg caaaaaatat ggtgctaaaa tcatactaac
    34021 ttcagatgct cacatttgtt ttgacatagg taactatgag tattctgaaa atatcttaaa
    34081 ggaaattaat tttccagatg aattaataat gaattatcct aaaaaactta ttaatcattt
    34141 tcatcaaaaa ggtaaattac tagatgttga ttatacaaat atctaagtaa taatacatgc
    34201 ttactttaac tcccataaaa attttaatac tatttatatt atatgaaata aagaaattat
    34261 aataaagaag ctatttaata aagcttaatt acttttatga acttgatgga ttgatatggt
    34321 ctatgatagc aatagaaatt ataacatttt taataagctt agttttatat accttatagt
    34381 ataaagctaa aaaaaagaat catctaaaat aaagtaaatc ttatataagg tttactttat
    34441 ttttatttgt aataatcatc aatacaaatt attttattat atgtaattat ttatgtctta
    34501 accaatttta tcgaaaataa cagtcttctc ttttaaagag atttttccta cttgatatcc
    34561 atattcattt aattcctttt tatatttcaa aaacgaatgg tctatctcta tcccagcaat
    34621 atcttttatt tcttcaaaag ttaatttaaa cgattgacta ttgtttttct taacatattc
    34681 ccataaagaa ctatatttac tcatcttata tcactccttt taaaatcata tcttatctat
    34741 taatttat
//
LOCUS       DAYAPA010000032        34144 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.032, whole genome shotgun sequence.
ACCESSION   DAYAPA010000032 DAYAPA010000000
VERSION     DAYAPA010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 34144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34144
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.032"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            191..1384
                     /locus_tag="ACGI59_001424"
     CDS             191..1384
                     /locus_tag="ACGI59_001424"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFG9739217.1"
                     /translation="MDTLKELFKIGSGPSSSHTMGPQRAAERFKNENPDAESFRAILY
                     GSLAATGKGHLTDYIIEKTIAPKKVEIVWEEDIIKDFHPNGMKFEALDKDKNVTAEWT
                     VYSVGGGTIAEEGQRNSKSNSIYHLDTMDEIVKWCKENNKTLVDFVLECEPKDIKDYI
                     KTIKDAMRKSIDDGLSTDEIIPGKLLLKRRASTFYNAYKKDKSFSTLVYAYALAASEQ
                     NASGNIIVTAPTCGSAGVIPGIFFAMQDFYNYDDEKIIEALLVAGIIGNIIKTNASIS
                     GAEVGCQGEVGAACSMAAAAVAYLKGGTIDHIEYAAEIALEHHLGMTCDPVYGYVQIP
                     CIERNAMAAQRAYDAANYALLTDGSHSVSLDQVVETMKETGIDMMDKYKETAKGGLAK
                     HFFSC"
     gene            1590..2708
                     /locus_tag="ACGI59_001425"
     CDS             1590..2708
                     /locus_tag="ACGI59_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyldiaminopimelate deacetylase"
                     /protein_id="HFG9739218.1"
                     /translation="MREASYLQEQLYKHRKSLNPIAEIGRKEYKTSKYIRDYLDKLGI
                     EYEVYLETGIVGVIEGKRPKKDIAFRADIDGLHSEEGVKHLCGHDGHATILLGLIEYL
                     NDNKEDLNDNIVFIFQPAEEGPGGAKALIDEGVLRKYNIDEIYGLHIYPELPEGYVGT
                     REGYFMAQIGDIDIEIVSKSGHGAMPQNGIDGIVIASNFVSSLQTIVSRNISPIDNAV
                     LTIGRIEGGARRNIIAENIKMEGTMRCFSPEVYEKMKLRVRELARGFELAYNCKVNIN
                     IIDDYIAVKNDKDLYQEFVEAIGSDTVVELEPLMISEDFSYYQKEVPGLFFMLGSRNE
                     EKGFVNGLHNINFNFDEKICVNALNVYSKLLKYKEAID"
     gene            2839..3084
                     /locus_tag="ACGI59_001426"
     CDS             2839..3084
                     /locus_tag="ACGI59_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small, acid-soluble spore protein, alpha/beta
                     type"
                     /protein_id="HFG9739219.1"
                     /translation="MKEKVQREKKITNGKNTKILTNDDIMKYEIASELGLMDKVGERG
                     WAGLTAKEAGRIGGMLTSRKKRLKKIVEEQNKKDESI"
     gene            3071..3817
                     /locus_tag="ACGI59_001427"
     CDS             3071..3817
                     /locus_tag="ACGI59_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFG9739220.1"
                     /translation="MSQYSDFAFVYDELMNEVDYNGWVKYIEDIIDSEGVKVKNILEL
                     ACGTGNLTIPLTKKNYDIAGIDISDEMLSVAREKAEKEGVELVLLQQDISELDFEISD
                     LDCVLCACDGFNYITYDDDLENVFSKTYELLKKDGIFIFDISSFYKLANILGNNMYGE
                     NREDIAYMWQNYFDEEENLIEMELTFFIKDECGKFERFEEIHQQRAYTEDEIVDMLEK
                     SGFKDIKYYGDFTFDKPSEESQRIFFVCKK"
     gene            4102..4977
                     /gene="hslO"
                     /locus_tag="ACGI59_001428"
     CDS             4102..4977
                     /gene="hslO"
                     /locus_tag="ACGI59_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFG9739221.1"
                     /translation="MRDYVLRATSGNGQVRAFVATTRNTVEEARRLHETTKVATAALG
                     RTLTATSIMGLMMKNDSDKLTVIIKGGGPIGTIIATSDSKGMVKGYVGNPQVEVEDYP
                     NGKLNVAAAVGTEGVVKVIKDLGLREPYNGTYPLVSGEIAEDFTYYFAVSEQTPSVVA
                     LGVLTKEDEVEFAGGFIVQLMPDAEEETIAKLEENVAKLPSITNMLKEGKSPEDILNI
                     VLDGLEPKILDTCEVGFMCECSKERVKTALVAIGKKSLAQIIEEDKKAEVGCQFCNKK
                     YMYSEEELLEILKEM"
     gene            5368..6168
                     /locus_tag="ACGI59_001429"
     CDS             5368..6168
                     /locus_tag="ACGI59_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HFG9739222.1"
                     /translation="MESKTKYIGISILILLMIFLIAKNFNGKLEIPIPESNSRKFKSA
                     AVFYPQHQDDEVLWGGSAIVDAIKQCGVDNVYVVLVSDGSGVNVFKANTKFRNLTRKQ
                     KEELRNNEFKSALRELGVKPQNVIILADIDKKEGTHYELMEKTILDFEHKFKGNVTHI
                     AHHYKYDDHIMHRKNGEVLKKLKREGKIKDDLYFVKPKYVKYIPRNERAIYKAMDFDD
                     YNKVKSACYEYKVVNSKRKMYGIGYISAHSYFEHLLNDPELTSVLSED"
     gene            6696..8936
                     /locus_tag="ACGI59_001430"
     CDS             6696..8936
                     /locus_tag="ACGI59_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFG9739223.1"
                     /translation="MNNEFIILKGCKENNLNNISLKIPKRKITIFTGVSGSGKSSIVF
                     ETIAKESQRQLNERFSTFVRSFLPKYGEVKADCIENLSTPIIIDQSRLGGNSRSTLGT
                     ITDVNSFLRALYSRFGSEYIGNANMFSFNNIDGMCPHCHGLGKKLVPNMNQILDMNKS
                     LNEGAILLSGFGVGSWHWNIFAESGFFDINKRICDYSEVELEKFLHGKAEKIKIENAG
                     QTNMTYEGLMVKFNRLYLSREGEISEATKKKLSKLLIEDRCPICDGRRLNERVYRSLI
                     NGYNIADLTSMQIDELAEVIKSIDEPEAQPLIKGIIEKLNSIIDIGLGYLTLDRETSS
                     LSGGESQRIKMVKYLNSNLVDLMYIFDEPSVGLHPKDVYKLNNLLKKLRDKGNTIIVV
                     EHDPDVIKIADHIVDVGPKAGKYGGEVVYEGSYENLLTSGTLTGNALSKFLPIKESVR
                     EHSGYLEVKNCNKNNLKNVSIKIPKGVLTLVTGVAGAGKSTLIKDEFLKQNPSAVLID
                     QSPVSANSRSSLATYSGIMNNIRSIFSKTNGVNASLFSSNSEGACDNCKGSGIVEMNL
                     AFMESIKSTCNICEGKKFKKEVLEYRFQGKNIIEVLEMSVLEAIEFFNLKQIKTKLQC
                     IEKMGIGYLTLGQTLDTLSGGECQRLKLASELNKESSVYILDEPTTGLHMADIENFIS
                     IIEEIVDNGNTVIIIEHNIDVIKRADWIIELGPEGGTKGGRVIFEGIPKQLCNNKISL
                     TAKYIV"
     gene            9350..10885
                     /locus_tag="ACGI59_001431"
     CDS             9350..10885
                     /locus_tag="ACGI59_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HFG9739224.1"
                     /translation="MINDILTLLSERSDFFIKLSIEHLMISIISIIIAIVLGGIIGII
                     VSEFHNSSKPILGTINFLYTIPSISMLGFLIPFSGIGNTTAVIALTIYALLPMVRNTY
                     TGITNVDKNILEAARGMGSTDFQILYKIKLPLALPVIMSGIRNMVIMTIALAGIASFI
                     GAGGLGVAIYRGITTNNTIMTITGSILIALLALIFDFILGLVEKFFQRKRKKKNRKKV
                     FAILGSLILIVIIFTSYLFNPSKTQTIHIATKPMTEQYILGEMLDILIEKNTGLNVEI
                     TQGVGGGTSNIQPAMENGEFDIYPEYTGTGWNMVLKKNELYTESMFSKLQSEYKTKLK
                     MQWTGMYGFSNSYALAVRKEVADKYNLKNITDLKDVSSKLTFGAEYDFYEREDGYTPL
                     CNTYGLNFKDTVDLDIGLKYQAINEGKIDVMPIFTTDGQYSVSDVVVLKDDKNFYPSY
                     QCGNVVRLEVLEENPELSKVFKMVGDILTDKEMAKMNYDVENNNLEPREVAREFLNKK
                     GLL"
     gene            10901..11662
                     /locus_tag="ACGI59_001432"
     CDS             10901..11662
                     /locus_tag="ACGI59_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFG9739225.1"
                     /translation="MTPIIQFKNIKKQYNDKTIIDNLNLDIEKGEFLTVIGSSGSGKT
                     TLLKMINGLILPDGGNILINQTDIKNEDLIKLRRRIGYCVQGSVLFPHMTVEENISYV
                     PNLLSKKNKLEVKSAVNKWMEIVGLPNDMKVRYPSELSGGQQQRVGIARALASSPEIL
                     LMDEPFGAVDEITRKQLQKEIKEIHKKTGVTIIFITHDIYEALILGTKTLVLNHGVVQ
                     QYDTPENILNTPANQFVDQLLNIRKSIIDDDNLKE"
     gene            12000..12869
                     /locus_tag="ACGI59_001433"
     CDS             12000..12869
                     /locus_tag="ACGI59_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFG9739226.1"
                     /translation="MDNNLITIEKTLDYIEKHLLDEMLDLNVISKEIGYSKYHLHRMF
                     TSVVGFSVHNYIQRRRLTEAARMLIFTEKPIIEIALISGYDTQRSFSRGFKKMFKRSP
                     NFFRRHQDFLPLQLKFDIHNRKKLRGDMVLDVKKVNCADINIVGYSGKVRKGFHIIGK
                     CWRDLHKNKEMIENKVDLGFLIGVSDYSNFKEIENTPTFTYIAGAQVATFGKLPNGME
                     KFVLPKSNYIVFTFIGKNEDSMQNVVEYIYQEWFPNSSCQFNENNLYDFVKYGEKTDE
                     NGLSEIQFWVPII"
     gene            13198..13449
                     /locus_tag="ACGI59_001434"
     CDS             13198..13449
                     /locus_tag="ACGI59_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HFG9739227.1"
                     /translation="MNVENELLKNLDRLHTTELGVVRIKKNLSLETNDVVNWCKTKIE
                     SPNAIINRKGKNWYISVYDCIITVNAHSYTIITAHKEKK"
     gene            13561..14340
                     /locus_tag="ACGI59_001435"
     CDS             13561..14340
                     /locus_tag="ACGI59_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFG9739228.1"
                     /translation="MNDTVKEKLAESLTSDTTDILPYLPYLLQDFWELGSSPEQMLQL
                     FRKHIPFNEDTKVLDLACGKGAVSVKLAKELNIHIKGIDLIPEFINYARRKAIEYKVD
                     ALCDFEMNDANSSVKIEHDYDCVIFGAVGDILGNYKETISKLLPTIKSKGFILIDDAY
                     VVDKSTNEQLNFERVYPTYNEWCILFKELGLKLIECIPCLELENDVEEEMNWIITRAN
                     ELIKKYPSQKDMFEQYVISQKNEYTDLQQDLVGATWILQKE"
     gene            complement(14475..15125)
                     /locus_tag="ACGI59_001436"
     CDS             complement(14475..15125)
                     /locus_tag="ACGI59_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9739229.1"
                     /translation="MNIVMINGSPKVNKNNSQYFLSELKQLIQNDNKVFTFKVDSTSK
                     YPDALNAILNCDRLIIASPLYVDGIPSHLLHFLEKCEKFLKTQNKKPIPVYAIVNCGF
                     NEGEHNHLAIQMIEHWCKKINFTWVQGIGIGAGEMFGSLESVPLGKGPKKDLGIALKE
                     LSKNILSDNIHIDDSNKSTIFISPNFPRFLYKLMGNHQWNTQAKSNGLTKSDILKK"
     gene            complement(15122..15625)
                     /locus_tag="ACGI59_001437"
     CDS             complement(15122..15625)
                     /locus_tag="ACGI59_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HFG9739230.1"
                     /translation="MRLLIHDLTDKELEKLLPKLDDNIKILPNTKEIHKCIGCFGCWI
                     KTPSECVVKDYYSNMGKLINDSDGIIIISRCCYGGFSPFVKNVLDKSINYILPYFVIK
                     DNMMRHKSRYDKQINLKAIFYGDCITEAEKETATKLVNGNAINFNIKEHSVCFYENIQ
                     DIRGESI"
     gene            complement(15674..16189)
                     /locus_tag="ACGI59_001438"
     CDS             complement(15674..16189)
                     /locus_tag="ACGI59_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFG9739231.1"
                     /translation="MLEYIILGFLMEKELSGYDLKQIMSESTSYFFDASFGSIYPALK
                     RLETKGYIHYHEVIDGSKLKKLYSITNTGKEVFLEWLKKPINFSKTKQDHLVNIFFYK
                     YLPKEIIETNLKMLITEVELLLNKLSKQKNDAEENHCVDQYTYMYATMIYGVDYYNFV
                     INWCKDLLKNL"
     gene            16469..16996
                     /locus_tag="ACGI59_001439"
     CDS             16469..16996
                     /locus_tag="ACGI59_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFG9739232.1"
                     /translation="MHEQIDYCERKKIRTIDIKGENMRELLKWVSIINRYNTNYIDRK
                     LADIGINNSQYFYVLHICNNPGITQDTIFEKILVNPSNITRALANLEKEGFITREPGV
                     KDKRTWHLYPTEKSLGCYDEILKITNGYVEELLSSFEKEEQELFMSMLKKVAFLAIDM
                     NEEAKLEAKENGRKI"
     gene            16980..18383
                     /locus_tag="ACGI59_001440"
     CDS             16980..18383
                     /locus_tag="ACGI59_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFG9739233.1"
                     /translation="MEEKYKEPLEYEKVSKLLTKYAIPSIIAMLVSALYNIVDQIFIG
                     QGVGVLGNAATNVTFPLTTICTAIALLLGIGGASKCSLELGAKNSEKATKAAGNSICL
                     MAVFGISLFIVVSIFLTPLLKFFGSTSEILPYAHTYTKITSIGLPFLIMSTGMSKLIL
                     ADGRPKASMFCMLIGAVINTILNPLFIFVFNMGIAGSALATVIGQLISFGISVHYIKN
                     FQHIKLHKKSFKLDSIIYKHIFALGSSSCFNQLAMTVTQIVMNNTLAYYGARSVYGSE
                     IPLACVGIIIKVNMIFISIIIGISQGMQPIIGYNYGAEKYNRVKEAYKFAVGSATIIS
                     VIAFLCFQFFPRQITSIFGNGNETYFIFAEHYFRTFLFLTFINGVQIVSSTFFTSIGK
                     SVLGLFTSLTRQILFLIPLIVILPMFMGIDGVMFAGPIADAAAAMVCIYFAVHEMKSL
                     TIKQNNLMSNHNKIAEV"
     gene            complement(18766..19077)
                     /locus_tag="ACGI59_001441"
     CDS             complement(18766..19077)
                     /locus_tag="ACGI59_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9739234.1"
                     /translation="MKKIIKSFTSWFFLIALFEIYMHQIGQDSKSIVLIGLNPILSII
                     SRVDSFFVFMDSGMQIPCRTITGSISIYWYIASILSFLIYGIILDLIRIVISKIGNKT
                     K"
     gene            complement(19133..19726)
                     /locus_tag="ACGI59_001442"
     CDS             complement(19133..19726)
                     /locus_tag="ACGI59_001442"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFG9739235.1"
                     /translation="MISDSISKRRSIRKYKNQSISHETIEKIIEAGINAPSSKNRQPW
                     RFVVITEKEKESMLKAMSKGIQNEINDNGLLPGSRQHIAGANYTVEIMKQAPVTIFIL
                     NILGKSPLEKLSPEERFYEMANMQSIGAAIQNMSLTAVELGLGSLWICDVYFAYRELC
                     EWLNTDSQLVAAISLGYPDEEPSRRPRLQLSDVTEWR"
     gene            20577..21227
                     /locus_tag="ACGI59_001443"
     CDS             20577..21227
                     /locus_tag="ACGI59_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HFG9739236.1"
                     /translation="MDFISSILQGLLSILFNFTGDFGISIVLITLIVKLVLMPLSLKQ
                     RFSVKKQQELAEKMEHIKEKYKNDTKELEKQIQVSSVESMKSMLGCLIVLVQMPVVYA
                     LYHVCLDMTKEFTTIIIPWIANLGTFDNLFILPCIYTLIMLASNLVNYIPYLKINSQV
                     KFNKQMAVVTTITSLILTVRTPVAIGLYFITSAIYSLIEDICFRIYFNRKNRIVTK"
     gene            21441..22112
                     /locus_tag="ACGI59_001444"
     CDS             21441..22112
                     /locus_tag="ACGI59_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFG9739237.1"
                     /translation="MAKILIVEDEKRMQSIIVEYMQKGGYTCITADDGVEALTILKSN
                     NIDLMILDIMMPYLDGFSVCRVSREMTNIPIIILTAKGEEEDKLKGYEYGADDYITKP
                     FSPKVLLAKVNALLRRYTTDIPKNSLSLGKIFIMVASRQVYVEDKLIDLTYKEFELLR
                     LFMENPNQVFSREKLLNCIWGYDFEGNTRTVDTHIKTLRKKLGSEGHHIVTLIRSGYK
                     FEVKE"
     gene            22109..23239
                     /locus_tag="ACGI59_001445"
     CDS             22109..23239
                     /locus_tag="ACGI59_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFG9739238.1"
                     /translation="MNSLRKIIEKLKNVDISNWIKQNFGLYSVRKKVFLLSKLSGALI
                     ILFYLVVEEFSINSKLEFWIWFIILVIFIMSIDFLLGKFISEPITSINKSAKSMSQLD
                     FSNPCTVNTNDEFGELSRSLNTMSTNLQQALSDLESANIQLEKDVNKERMLLEQRKEL
                     VDTISHEMKTPLGIIRAYTEGLMDEVDEEKKKNYMNVIIEETDRMNNMIVSLLDLSAL
                     EAGVSKLNPERFDFIEFVETVAGRLLIDIPDANFYFTYDLPEYEVFVVADKMRMEQVV
                     ENLVINAKKHVVYNGNLDLSITCENGLLLFEIYNDGRFIEQDEISKIWSKFYRSVQSQ
                     RTGGSGLGLAIVSQILTMQGLEYGVNNRDRGVEFYFMIPIDE"
     gene            23540..23662
                     /locus_tag="ACGI59_001446"
     CDS             23540..23662
                     /locus_tag="ACGI59_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9739239.1"
                     /translation="MKWYTWLTIILIILLSIPLKIKFIKWWDRRQKNDKGGYDR"
     gene            23659..24504
                     /locus_tag="ACGI59_001447"
     CDS             23659..24504
                     /locus_tag="ACGI59_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9739240.1"
                     /translation="MIKVGELKFSYGKDKQILHGLNFEVKEGEIFGFLGPNGSGKSTT
                     QKILNGVLKGYNGHVSLFGKEVKAYTESLYQKIGVLFEFPYLYTNLSAIDNLEYFSSF
                     YPKNQRRDIYELLDLLEFKKEFINKPVSSYSKGMKQRISMARALVSNPKLLFLDEPTS
                     GLDPSGAVLFRKIIEEERKKGTTIFLTTHNMLDADLMCNNVAFIADGKIMVIDKPKNL
                     KMKNSNNKVEVEFVYNGNRDIEIVDIQELESGITFKYDEILSVHSKEPTLEEVFIKYT
                     GRMLV"
     gene            24504..25532
                     /locus_tag="ACGI59_001448"
     CDS             24504..25532
                     /locus_tag="ACGI59_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9739241.1"
                     /translation="MYSKFFTLVKKDFWMMIYGKFFVLTLGSLILYSCYINFGYVKFM
                     DEDSYHNNVYLYNPENISMSASPLINQVSSIGELKAKLDEDTSAVCIDMSSSKPKVIL
                     HESIKGVDDYRADYALSLLSSEKDHNSKISDNNAIIQINDKDTSEIKQRKDMTCEILF
                     FEIVAIGFLGIASLLFKEKQMGVMRITGILPVKRSLFILSKIFAFLICDLCFTVLLVV
                     FNIGFFEGIKILPQVLVQASILSIIMSLIGIGCSMLLRDFKQFSLIYLVVAVFITTPI
                     FLSANTSFEIDWIKYHPFYHVYSQLKNAFFMNFSKELVYFVVCSLTIIVLFLLVKYIF
                     NKELVREG"
     gene            25535..26548
                     /locus_tag="ACGI59_001449"
     CDS             25535..26548
                     /locus_tag="ACGI59_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9739242.1"
                     /translation="MIGLRYQLKSIKKDKMCIISFFLPVVMAILINFMGTIDISSLGE
                     IRFGIVKDTLPVQTEQWLKKYGMVSVYDTKMHLIREIKDIKTDTIGVVRDGKGIKTVL
                     YGNEINAIKNVGKTLPEMFRLKGDLKDVEVTILPKDSILKEFQNVFIAMILITAMFMG
                     CTFNAMNIISEKEDGISFINEVLPQSRSQYIIQKVFLGLLCGCLSSMITVFICLDISI
                     LDMIMMLLLVVFSAFISSLIGLFIGKLSEGLMVGIVYIKIVMILFIGTPLLIYLLGVN
                     TSGIGNLYYIMPSVATFNGIMEIINGNVIIIKDIVILAVHCVVWFMIYIILDKKRKLF
                     FSR"
     gene            complement(27065..28315)
                     /gene="cme"
                     /locus_tag="ACGI59_001450"
     CDS             complement(27065..28315)
                     /gene="cme"
                     /locus_tag="ACGI59_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Cme"
                     /protein_id="HFG9739243.1"
                     /translation="MENKNTNSTWKKKVILFLASQYISLFGSSLVQMAMIWYVTLQTS
                     SGVWVTVLTVCSFIPQMIISFFAGVWADYYNRKVLIIVSDSTIAISTLVLILFMMSSN
                     GTTSELIAISVISVIRSLGSGVQTPAVNAMIPQLVPEEHLMRFNGINSSLQSIVQFIV
                     PIVAAAILGFGNKINQVLLIDVFTAIIAIVLLIFIKIPKHDIPKQTEKVSFFADMVSG
                     IKYCFSDKFIRKVLFTYGAFMLLCVPSGFLIGLLIERTFGKGYIYLSIIEMIGFLGMV
                     LGGLIIGVFGGFKNRNKTFFLAILSYAIFSIILGLVTQAWQFFIFMFFTNFSIPIIQA
                     SAMTMLQEHVAPQMLGRVLSLPIIIYTGFIPLGMMIFGPMADVISINNLIIIAGIILL
                     FFALAIPYSKQFYKQGISKSNESE"
     gene            complement(28401..29147)
                     /locus_tag="ACGI59_001451"
     CDS             complement(28401..29147)
                     /locus_tag="ACGI59_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFG9739244.1"
                     /translation="MDTYTTSEVADLIGVHPNTVRLYEELNLIPKPKRNTNGYRIFND
                     FHIEQFRFARTALKIEVVQRGLRKDALEIVRTSANRDFDKAISLVNQYLQQLEIEKSN
                     AEEALTIVAQILNGNTPETNKLFLTRKETAEHLNVTIDTLRNWEMNGLLKVKRKQNGY
                     RVYTDEDIKRLKIISALRCANYSLSAILRMLNALSSNPNTNIKTVLDTPKDDDEIISV
                     CDKLITSLKEAEHNAKTMKSHLQIMKKQFS"
     gene            complement(29262..30284)
                     /locus_tag="ACGI59_001452"
     CDS             complement(29262..30284)
                     /locus_tag="ACGI59_001452"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFG9739245.1"
                     /translation="MNKNNCLDLIFEKVNPSSVGMSSEKLSQLEPLIISQYSNINGII
                     IARKGKVVFEKYYNGYNADDTFHVASVTKSIISALIGIAIDKGYIKDVDQKVVDFFPE
                     YTYSPSEICKKAVTIRHLLTMTAPYPFKNMTESLERLCKQKDWIKYTLNQLGQKGEIG
                     TFKYSSAGTHLLSAIITKTTGKSAREFANEYLFKPIGMKVIPDYDMKDFGFEDFFGKN
                     LKGWAKDRQGYSIGGWGLTLSARDMLRFGFLYLNDGIWNNKRIISKSWIEESVKMNSN
                     EYGYLWWLCKKDGVLWYSAMGAGGNAICCIPQYDLVIAIPSKIVFKPRDRWEFIEKYI
                     LTSIID"
     gene            complement(30284..32704)
                     /locus_tag="ACGI59_001453"
     CDS             complement(30284..32704)
                     /locus_tag="ACGI59_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFG9739246.1"
                     /translation="MKEIKINGAKIHNLKNIDVSIPKDKLVVATGVSGSGKSSLMFDI
                     VFEEGRKQYLQSLGILAGIDSEDKFDNIQGLAPTIAVQQNIIRQSNPRSTVGTRTNIL
                     NMLTLLYSVEGQIMCTMCDTPVSYNLICNNCGYEEERLRPSYFSYNSSDGMCMKCSGH
                     GAYFEINIEKLVLDKHDTLKQVLDRAKITPGYLRVFSKKFNDYLDIQFLQLPEEVRNE
                     VLYGHYENGKKSSSLSKVFHNRYKRGEDLNGVYTMTACSDCNGFKIGEEARRVLLNGK
                     HIGELGKMTILEIDDFLKLLINQGNLNTFGTNLVNDILSKTQHLIESRLGHLSLYREM
                     STLSGGEMQRLFLNSHLDSEMDSLIYVLDEPTAGLHESEKIDILKSIKKLKDLGNTVI
                     VVEHDKNTIKMAEHIIDIGPKAGVEGGQLVYQGDLEGLLQSDKSLTGQYLSGKYPMPN
                     RTSNKNITHTDKIPCITIQNANTNNLKNVTVSLPLGAMVGISGKSGSGKSSLISDTLL
                     PLLRSQFNNHTINNQINSSESEIGEEDDYAVVETIADRLIGTKHISGYSEISQSPIGK
                     NMNSTPASYIGIWDKIRTLFAGQPESLERGFTAGHFSFNSKGACPKCSGSGYEKVWLG
                     NNLSIDHICSECQGKRFNDESLSIKYKNKNIHDILNMSVSESVHFFKDIPNIVSHLNV
                     LEQIGMGYIKLGQPTPTLSGGESQRIKLAKEIGKKRNGNILYVLDEPTTGLSLYDTSK
                     LIQLLDELVENGNSVIVVEHDIDVLNVCDWIVELGPEGGDKGGYIIAEGSPKTLKENP
                     KSITGRYL"
     gene            complement(32790..33710)
                     /locus_tag="ACGI59_001454"
     CDS             complement(32790..33710)
                     /locus_tag="ACGI59_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFG9739247.1"
                     /translation="MTNAKSKKEYLNRICKVQDYIEEHLHEPLSLDELSNIAGFSKFH
                     FHRIFKAIEKETLAQYVNRLKLENATAFLIHRTDMTVTDIAHYFGFTDSAVFSRAFKN
                     YYKISPIKYRNNYSKNCKEPYKISQYNRSISKPKCKNTREVKGEIEILELDNMNTIYT
                     RYTGSYGNLTSTFPKLLERLFTYASEQNLLGLENTQILAIYHDNPQFTQEHQLRTSIC
                     MTIPDNIVVKENDNLGNMIIPSGKYLVGHFDISKSEYSDAWDFMYNEWLSNGNYKLRA
                     SFPFEVYLNNPHNHPQNRHLVDIYLPIEPF"
BASE COUNT        12640 a         3982 c         5750 g        11772 t
ORIGIN      
        1 ttagaaaaat tttaaataaa tgttttattc tcaaaatatg catattaatt taataaaata
       61 taccaaaatg gaaataaaaa ataaatttta tgagaattta ataactaata attaatatat
      121 ttaggttata ataaatatgt gcgtaaaatc taacaataag caataatacc gtaaatttag
      181 gaggaataat atggatactt taaaggaact ttttaaaata ggaagtggac cttcaagttc
      241 tcatactatg ggaccacaaa gagcagcaga gagatttaag aatgaaaacc cagatgcaga
      301 aagttttaga gcaatattat atggtagttt ggcagctaca gggaaaggtc acttaactga
      361 ctatataatt gaaaaaacta tagcacctaa aaaagtcgaa attgtttggg aagaagatat
      421 tataaaagat tttcacccaa atgggatgaa gtttgaagct ttagataaag ataaaaatgt
      481 aacagctgaa tggactgtat attctgtagg tggagggact atagcagaag aaggtcaaag
      541 aaacagtaaa agcaatagta tatatcactt agatactatg gatgagatag taaaatggtg
      601 caaagaaaat aataagactt tggtagattt tgtgttggaa tgtgaaccaa aagacataaa
      661 agattatata aaaactataa aagatgctat gagaaaatct attgatgatg gactaagtac
      721 agatgaaata ataccaggca agttattatt aaaaagaaga gcaagtacat tttataatgc
      781 atataaaaaa gataagagct tctcaacact tgtatatgca tatgcactag cagcttcaga
      841 gcaaaacgct tctggtaata taatagttac agcaccaact tgtggttctg ctggagtaat
      901 accaggaata ttctttgcaa tgcaggattt ctataattat gatgacgaaa aaataattga
      961 agcactactt gtagctggaa taataggtaa tataataaaa actaacgctt caatatctgg
     1021 agcagaggta gggtgtcaag gtgaagttgg agccgcttgt tctatggcag cagcagcagt
     1081 agcatactta aaaggtggaa ctatagacca tattgaatat gcggcagaaa tagcacttga
     1141 acatcattta ggaatgactt gtgaccctgt atatggatat gttcaaattc catgtataga
     1201 aagaaatgca atggcagctc aaagagctta tgatgcagct aattatgcat tattaactga
     1261 tggctcacat tctgtctcct tagaccaagt tgttgaaaca atgaaagaaa ctggtataga
     1321 tatgatggat aagtacaaag aaacagctaa gggtggactt gctaaacatt tcttctcttg
     1381 ctaaaaataa aattttccaa taataattcc tctgttttga tttatgaaac aggggaatta
     1441 ttattaatgg tgaattattt aataatgcaa aatattttta tattcaattt agctttttat
     1501 ttttaattag tataacatgc tatataatat tttaattata aagaaatata gaaatatttt
     1561 ttaataaatt agaaattgga ggagattaca tgagagaggc atcatattta caagaacaac
     1621 tttataaaca taggaagtca ttaaatccta ttgcagagat tgggagaaaa gagtataaga
     1681 cttcaaaata tataagagat tatcttgata aattaggaat tgagtatgaa gtttatttag
     1741 aaacaggtat agtaggtgta atagaaggta aaagacctaa aaaagatatt gcttttagag
     1801 ctgacataga tggtcttcat agtgaagaag gggtaaaaca tttatgtggt catgatggac
     1861 atgctactat attacttgga ttaattgagt atttaaatga taataaagaa gacttaaatg
     1921 ataatatagt atttatattc caaccagcag aagaaggtcc tgggggagca aaagctttaa
     1981 tagatgaagg agtgcttaga aaatacaata ttgatgaaat atatgggctt catatatatc
     2041 cagagttacc agaaggttat gtaggtacaa gagagggcta ttttatggct caaataggag
     2101 atattgacat agaaatagtg tctaaaagtg gacatggggc tatgccacaa aatggtattg
     2161 atggtatagt gatagcttca aactttgtaa gctcacttca aactatagtg tctagaaata
     2221 taagcccaat agacaatgca gtgttgacta ttggtagaat tgaaggggga gctagaagaa
     2281 atataatagc tgaaaatata aagatggaag gtactatgag atgtttcagc ccagaagtgt
     2341 atgagaaaat gaagcttaga gttagagaac ttgctagagg gtttgaattg gcttataact
     2401 gtaaggttaa tataaatatt atagatgatt atattgctgt taaaaatgac aaggatttgt
     2461 atcaagagtt tgtagaggct attggaagtg atactgttgt agagttagag ccacttatga
     2521 tatcagaaga tttttcttat tatcaaaaag aagttccagg attatttttt atgttgggtt
     2581 caagaaacga agaaaaagga tttgtaaatg gtcttcataa tataaacttt aattttgatg
     2641 aaaagatatg tgtaaatgca ttgaatgttt attcaaaatt attaaaatac aaagaagcaa
     2701 tagattagga agataagtta taaagattaa aaagcataaa tacctaaatt taaaaaaagt
     2761 gtattaataa atacccctta catatttgta taaatttatt ttaattgaca aaaatacaaa
     2821 taagaagggg tgattttttt gaaagaaaaa gtacaaagag aaaagaagat aactaatggc
     2881 aagaatacta aaatattgac aaatgatgat ataatgaaat atgagatagc ctcagaacta
     2941 ggtcttatgg ataaagttgg tgaacgtgga tgggcaggac ttacagctaa ggaagcagga
     3001 agaattggag gtatgcttac atcacgcaaa aagaggctta aaaagattgt ggaggagcag
     3061 aataaaaagg atgagtcaat atagtgattt tgcgtttgta tatgatgaac ttatgaatga
     3121 agttgattat aatggttggg ttaaatatat agaagatata atagatagtg aaggtgtaaa
     3181 agtaaaaaat atattggaat tggcatgtgg cacgggtaat cttacaattc cattgactaa
     3241 aaaaaattac gatatagctg gtatagatat atctgatgag atgcttagtg tagcaagaga
     3301 aaaggcagaa aaagaaggag tagaattagt tttacttcaa caagatatat cagagcttga
     3361 ttttgaaata tctgatttgg actgtgtact ttgtgcttgt gatggattta attatataac
     3421 ttatgatgat gatttggaaa atgtattttc aaagacatat gaattattaa agaaagatgg
     3481 aatattcata tttgatataa gttcttttta taaattagcc aacatattgg gaaataacat
     3541 gtatggagaa aatagagaag atatagcgta tatgtggcaa aattactttg atgaagaaga
     3601 gaatctaatt gaaatggaac ttacattctt tattaaagat gaatgtggaa agtttgaaag
     3661 atttgaagag atacaccaac aaagagcata tactgaagat gagatagttg atatgcttga
     3721 aaaatctgga tttaaggata taaaatatta tggggatttt acatttgaca aacctagtga
     3781 agaaagtcaa agaatcttct ttgtatgtaa aaaataagat attcttgact atataagata
     3841 attattaaaa ataaccatga tataaaaata tataaaatgc agttttatag attaaaagta
     3901 aagtgatgat attttatgat ttttattttc taataattat aaagttttat agataaaaat
     3961 taaaatataa taactattgc ttagtaaaat taaaataaaa tgggttttgc ccaaggttaa
     4021 gatagataag tatttataga ttaaaattaa ggtagtaata taataaaaat aagacaatta
     4081 acgggacatg gaggaatatt aatgagagat tatgtactaa gagcgacatc aggaaatggt
     4141 caagttagag catttgtcgc aactactaga aatacagtag aagaagctag aagattacat
     4201 gaaactacaa aagtagcaac agcagcactt ggaagaacat taacagccac atctataatg
     4261 ggattaatga tgaaaaatga tagtgataaa ttgactgtaa taataaaagg tggaggtcca
     4321 attggaacta ttatcgccac atcagactct aagggaatgg taaaaggata tgtaggaaat
     4381 ccacaagtag aggttgaaga ctatccaaat ggaaagttaa atgtagcagc agcagtaggt
     4441 acagaaggtg ttgtaaaagt tataaaagac ttaggtctta gagaaccata taatgggact
     4501 tatccattgg taagtggaga aattgcagaa gactttactt attattttgc tgtatcagag
     4561 cagactccat cagtagtagc tttaggtgtt cttacaaaag aagatgaagt agagtttgct
     4621 ggaggattta tagttcaatt aatgccagat gcagaagaag agactattgc taagttagag
     4681 gaaaatgtag ctaaattacc ttctataact aacatgttaa aagaaggaaa atcaccagag
     4741 gatatattaa atattgtttt ggatggtttg gaacctaaaa tacttgatac ctgtgaagtt
     4801 gggtttatgt gtgaatgttc aaaagaaaga gtaaaaactg ctttggttgc aattggtaaa
     4861 aaatcattgg ctcaaataat agaagaagat aaaaaagcag aagttggttg ccagttctgt
     4921 aataagaagt atatgtatag tgaagaagag ttgcttgaaa ttttaaagga aatgtagact
     4981 ataaaaatag ttgtattgat ataagtaatg ttatagtaat atagtaaagt cgtattgata
     5041 tagtaattaa agtataattt attaataata gacatattta tattaaaaaa taagtttaat
     5101 aaatttttaa gcttaataaa ttttaagaat taagaagctt tctggctgta atgtttgcaa
     5161 tgttgcagtt agaaggcttt tttattttat tatagttcgt gtcaaagatt gtatgaaaca
     5221 ataaaatatt gtaaaataat ttgtatattt agacctgatt gacacaaagg tttataagat
     5281 atttgagttg attatacgaa attctatgaa gtagtaaccc tgtgacttta atcatgggag
     5341 gttcagaaga aggagaaaat tttaaatatg gaaagtaaaa cgaagtacat aggaataagt
     5401 atattaatat tattgatgat atttttaatt gcaaagaatt ttaacggaaa acttgaaata
     5461 cctattcctg agagtaatag tagaaaattt aaatcagcag cagtgtttta tcctcaacat
     5521 caagatgatg aggttttatg gggaggaagt gccattgtag atgctataaa acagtgtggt
     5581 gttgataatg tatatgtggt attagtatca gatggttctg gagttaacgt tttcaaagct
     5641 aacacaaaat ttagaaattt aactaggaag caaaaggaag aacttagaaa taacgagttt
     5701 aaatcagctt tgagagagct tggagtgaaa cctcaaaatg tgattatact tgcagatata
     5761 gataaaaagg aaggcacaca ttatgaatta atggaaaaga ctatattgga ttttgaacat
     5821 aagtttaaag gcaatgtgac ccatatagcc catcactata agtatgatga ccatataatg
     5881 catagaaaga atggggaagt tttaaagaaa cttaaacgtg aaggtaagat aaaagatgac
     5941 ttgtattttg ttaagccaaa atatgtaaag tatataccaa gaaatgaaag agctatctat
     6001 aaagcaatgg attttgatga ctacaataag gttaagagtg cttgttatga atataaggtt
     6061 gtaaattcaa agcgtaaaat gtatggtata ggatatattt cggcacatag ttattttgaa
     6121 catcttctaa atgaccctga acttacgtct gtattaagtg aagattaatt tcttataagt
     6181 agtaagttat gtttgataag ttgattggaa ttttctatag ttttgaaggt attggaaatt
     6241 tagttttatt taaagattta aagatagagg tttttagtgt ttaaaatttt aaagttaagt
     6301 tgaataattg tatagaatta tttgtgaaag aagaaacagt gtaaaacata gtatcttatt
     6361 tatggtttta aaatcatagg caagatactt tttttatgta actaatacca ttttgagatt
     6421 attaacttaa gaattttata taatttaaca ctcatatata atgatgatat ttaggtagaa
     6481 tagatgtaat tacgcttaaa taaaataact gtatttttta taaaaatcca aaccctacac
     6541 tatactacca gataaaatct acatgctata atttgacctg tgtttataaa aaaaggagta
     6601 atctaataaa atagtatttc acttatgtat aactaacaac tattattacg attttctcta
     6661 aataatttaa agaaaaataa tgcaggaggt aaaatatgaa caatgaattt ataattttaa
     6721 aaggttgcaa agagaacaat ttgaacaata tttccttaaa aataccaaaa agaaaaataa
     6781 ccatattcac aggggtgtcg ggttctggaa agtcatctat tgtatttgag acaattgcaa
     6841 aagaatcaca acgccaacta aatgaaaggt ttagtacatt tgtaagaagt tttttaccta
     6901 agtatggaga ggttaaagct gattgtatag aaaatttgtc aacaccaatt atcatagacc
     6961 aaagtagact tggtggaaat tcaaggtcaa ctctaggtac tattacagat gtaaattcct
     7021 ttttaagagc tttatattca aggtttggta gtgagtatat aggtaatgcc aatatgtttt
     7081 cttttaataa tattgatggt atgtgcccac actgtcatgg gcttggaaaa aagctagttc
     7141 ctaatatgaa ccaaattctt gatatgaata aatcattaaa tgaaggtgct atattgcttt
     7201 cagggtttgg agtgggttct tggcattgga atatattcgc tgaatcagga ttttttgata
     7261 ttaataagag gatatgtgat tattcagaag tagaattgga aaagtttttg catggtaaag
     7321 cagaaaagat taagatagaa aatgctggtc aaactaacat gacatatgag ggattaatgg
     7381 ttaaatttaa tcgcctatac ttaagtagag agggagaaat atctgaagct actaagaaaa
     7441 aattgagtaa acttcttata gaagatagat gtcctatatg tgatggaaga agacttaatg
     7501 aaagagtata taggtcttta attaatggtt ataatatagc agatttaact agtatgcaaa
     7561 ttgatgaatt ggctgaagtt attaaaagta tagatgagcc agaagcacaa cctttaatta
     7621 aaggaataat agaaaagtta aacagtatca tagatatagg tcttggatat ttaacactag
     7681 atagagagac atcttctcta tcaggagggg aatctcagcg tataaagatg gttaagtatt
     7741 taaatagtaa tcttgttgat ttaatgtata tttttgatga accaagtgta ggacttcatc
     7801 caaaagatgt gtataaatta aataatcttt taaaaaaatt gcgtgataaa gggaatacaa
     7861 ttatagttgt tgaacatgac ccagatgtta ttaagattgc agaccatatt gtagatgtag
     7921 gaccaaaggc aggtaagtat ggtggagaag ttgtgtatga aggcagttat gaaaatctac
     7981 ttacaagtgg tactttgaca gggaatgctt taagtaaatt tcttcctatt aaagaaagtg
     8041 taagagaaca tagtggatat ttagaagtta aaaattgtaa taaaaacaat ttaaaaaatg
     8101 tatctattaa gatacctaaa ggtgtattga ctttagttac tggagtagca ggagcaggta
     8161 aaagtacatt gattaaggat gaatttttaa agcaaaatcc tagtgctgta cttatagacc
     8221 aaagccctgt ttcagcaaat tcaaggtcga gtttggcaac atatagtgga attatgaaca
     8281 acataagaag catattttca aaaacaaatg gcgtaaatgc ttctttattc agttcaaatt
     8341 cagaaggagc ttgtgataac tgtaagggtt ctggaatagt agaaatgaat cttgctttta
     8401 tggagagtat aaagagtacc tgcaatattt gtgaaggtaa aaaatttaaa aaagaagttt
     8461 tagaatatag gtttcaaggg aagaatataa ttgaagttct tgaaatgtct gttttagagg
     8521 caattgagtt ttttaattta aaacagatta aaacgaaatt acaatgtatt gaaaaaatgg
     8581 gtattggata ccttacttta ggacaaactc ttgatacttt gtctggagga gagtgtcaaa
     8641 gattaaagct tgctagtgaa ttaaataagg aaagttcagt gtatatatta gatgaaccaa
     8701 caacaggtct tcatatggct gatattgaga attttataag tataattgaa gagattgtag
     8761 ataatggaaa tacggtcata ataattgaac acaatataga tgtaattaaa agagctgatt
     8821 ggattattga gctgggacct gaaggaggaa caaaaggcgg gagagtaata tttgaaggta
     8881 ttccaaaaca attatgtaac aataaaatat cattaacagc aaaatatata gtataataaa
     8941 taaaatataa taaaattata atgatatttt aataaatata atgggtatat aaaatgataa
     9001 ttaaacttaa cagtaataaa taatgtgaga aatattcaaa tttaattaaa taaagtgtca
     9061 gaaatttgtt taaattttag tattttaacg gttctgacct tttttcttat ttttatggag
     9121 aatatcgagg atggagtctc taatagttac ttttttgtgt ctacttgcaa gtttgattaa
     9181 aaaatggcta tacacacttg tgacttcagt catgggaggt tcagattata atcttaatat
     9241 ataatttgtg aaataatttg atgacgattt gctgatgtat aaacaataaa gattaactac
     9301 attaaaattt aaatcttgtt aagtaaaact tataaaagga gagagatgga tgataaatga
     9361 tattctaacc ttattatctg aaagaagtga tttttttata aagctatcca tagaacattt
     9421 gatgatttct attatttcta taataattgc gattgttttg ggtggtataa ttggtattat
     9481 tgtaagtgaa tttcataatt cttctaaacc tatattggga actatcaatt ttctatacac
     9541 cattccatct atttctatgc taggatttct gataccattt tccggaattg gaaatactac
     9601 tgctgttatt gcacttacca tttatgcatt attgccaatg gttagaaata catatactgg
     9661 aattactaat gtggataaaa atattcttga agcagctagg ggaatgggaa gtacagattt
     9721 tcagatattg tataaaataa aacttccgtt agccctacct gttattatgt ctggtattag
     9781 aaatatggtg attatgacaa ttgcattagc tggtatcgct tcatttatag gtgctggagg
     9841 cctaggtgta gccatatata gaggaatcac gacaaataac acaataatga caattactgg
     9901 aagtatactt attgctctat tagctttaat atttgatttt attttagggc tggtagaaaa
     9961 attttttcaa cgaaaaagaa aaaagaaaaa taggaaaaaa gtttttgcca ttctaggaag
    10021 ccttattctt atagtaatta tctttacgtc ttatttattt aatccttcaa agactcaaac
    10081 tattcatatt gctactaaac ctatgactga acaatatatt cttggtgaga tgttggatat
    10141 tttaattgaa aaaaacacag gtctaaacgt ggaaattaca caaggtgtag gagggggaac
    10201 atctaatatc cagccagcta tggagaatgg agaatttgac atttatccag agtatacagg
    10261 tacaggctgg aatatggtac ttaagaaaaa tgaattatat actgaaagta tgtttagtaa
    10321 actacagtcg gaatacaaaa ctaaacttaa aatgcaatgg actggtatgt atggtttttc
    10381 taatagctat gcattagcag ttagaaaaga agttgcagat aaatacaatt taaaaaatat
    10441 aacggactta aaagatgtat cttctaaatt gacatttgga gctgaatatg acttttatga
    10501 gagagaggat ggatatactc cattgtgtaa tacatatgga ctgaacttta aagatacagt
    10561 tgatttagat attggattaa aataccaagc tatcaatgaa ggtaagattg acgtaatgcc
    10621 tatatttaca actgatggtc agtatagtgt ttcagatgtt gtagttctaa aagatgataa
    10681 aaatttctac ccatcttatc aatgtggaaa tgttgttcgt ttggaagtat tagaggaaaa
    10741 tccagaacta tcaaaggtat ttaagatggt tggagatatt ttgactgata aagaaatggc
    10801 taaaatgaat tatgacgtag aaaacaacaa tttagaacct agagaagttg ctcgtgagtt
    10861 tctgaataag aaaggtcttt tataaattgg aggaaatata atgacaccta ttatacaatt
    10921 taaaaatatt aaaaaacaat ataatgataa aactataata gataatctta atttagatat
    10981 agaaaaagga gagtttttga ctgtaattgg ttcgtctggt tctggtaaga caacactctt
    11041 aaaaatgatt aatggtctga ttttgccaga tggaggaaat attttgataa accaaacaga
    11101 tatcaaaaac gaggacttaa ttaaattaag aagaagaata ggatattgtg tacagggaag
    11161 tgttcttttt cctcatatga ctgttgaaga aaatatttca tatgtaccaa atctcctaag
    11221 taaaaagaat aaattagaag taaaaagtgc tgtaaacaag tggatggaaa tagtgggatt
    11281 gccaaatgat atgaaagtgc gttacccatc agaattgtca ggtggtcagc aacaacgtgt
    11341 agggattgca agagctttag cttcttctcc agaaatttta ttaatggatg aaccttttgg
    11401 tgcagttgat gagattacac gaaagcaatt acaaaaagag ataaaagaaa ttcataaaaa
    11461 aacaggagtt acgattatct ttattacgca tgatatttat gaagctttga ttttgggaac
    11521 aaagactttg gtattaaatc atggagttgt tcaacagtat gatactcctg agaatatttt
    11581 aaatactcct gctaaccaat ttgtagacca gttattgaat ataagaaaat ctataataga
    11641 tgatgataat ttaaaagaat aaatttaaat ttaaaatcaa attgttgtga taaaaactat
    11701 tgatttagag ctaacttcaa gttgtatact tgaagtggaa aagttagatt aaggtataga
    11761 agggcattta gcagttggct gtagacgagg taactgctga aggtgctttc aataaatggc
    11821 tagaacctgt atctgttgaa gattataata aattgaaata agaagctcaa tatacagagt
    11881 agataggcac ttcaaataga agtgtctttt gtgtgccata aaataaatca aaatattagc
    11941 aaagaatgtg aaaaacaaaa taaaataata tgctatcttt aaaataggag ggatgctata
    12001 tggataacaa tttgattacc attgaaaaaa ctttagatta tatagaaaaa catcttttag
    12061 atgagatgtt agatttaaat gtaatttcta aagagatagg ctattcaaaa taccatttac
    12121 atagaatgtt tacatcagtt gtcggattta gtgtacataa ttatatacaa agacgtagac
    12181 ttactgaagc agcgagaatg ctaattttta cagaaaaacc aattattgag attgctttaa
    12241 tctcggggta tgatacacaa cgctcttttt caagagggtt taaaaaaatg tttaaacgta
    12301 gtcctaattt ttttagaaga catcaagatt ttttaccact acaattaaaa tttgatattc
    12361 ataatagaaa aaaattacgt ggagatatgg ttttagatgt taaaaaagtt aattgtgcag
    12421 atataaatat tgttggctat tctggtaaag taagaaaagg atttcatatt attggaaaat
    12481 gctggaggga tttgcataaa aataaagaga tgatagaaaa taaggtagat ttaggttttt
    12541 taattggagt tagtgattat agcaatttta aagagattga gaatactcct acatttacat
    12601 acattgcagg tgctcaagta gctacttttg gcaaattacc taatggaatg gaaaaattcg
    12661 tattaccgaa aagtaattat attgttttta cttttattgg gaaaaatgag gatagtatgc
    12721 aaaatgttgt agaatatatc tatcaagaat ggttccctaa ttcaagttgc cagtttaatg
    12781 aaaataattt atatgacttt gtaaaatatg gagaaaaaac tgatgagaat ggtttgagtg
    12841 aaattcagtt ttgggttcca atcatttaaa agaaggtatt atgtataaag tagatggatt
    12901 tatataggca aagttttaca gcagtatgta tttataaata atttatagtt tgtaggaaag
    12961 aaataatttt actataagat tatgataaaa tacacaatac tatgacaaaa tagtgttaaa
    13021 taatagattt aattttgcat aggaaaagtt ctataatggt gtgtattcat aaataattta
    13081 taatctgtag gaaagaaata gttttactat aagattatga taaaatacac aatactatga
    13141 taaaatagta ctaaataata gatttaattt tgcaaaggat aaggtgtttt aataaaaatg
    13201 aatgtagaaa acgaattact taaaaaccta gatagattgc atacaacaga gttaggcgtt
    13261 gtaagaatta agaaaaatct ttctttggag acaaatgatg tagtgaactg gtgtaaaact
    13321 aaaatagaat ctccaaatgc cattattaat agaaagggaa aaaactggta tataagtgta
    13381 tatgattgta ttataacagt aaacgctcac agttatacaa ttattacagc tcataaagaa
    13441 aagaaatgaa ttgcaaaata caaaggtcaa aagcattttc cactgtttgt agtacctatg
    13501 aagaagtata taaaataata ttacttttga ataataattt aaattttgga ggttgaaaaa
    13561 atgaatgata ctgtaaaaga aaaattggca gagtcactaa cttctgatac tactgatatt
    13621 ttaccttatt tgccttattt attacaagat ttttgggaat taggaagttc accagaacaa
    13681 atgttacaat tatttagaaa gcatatacct tttaatgagg atacaaaagt tttggattta
    13741 gcttgtggaa aaggtgctgt atctgtgaag cttgcaaaag agttgaatat acatatcaaa
    13801 ggtattgatt tgatacctga atttattaat tatgctagaa gaaaggctat tgaatataag
    13861 gttgatgctt tgtgtgattt tgaaatgaat gatgctaata gttctgtaaa aattgagcat
    13921 gattatgatt gtgtgatttt tggagctgta ggggatattt taggtaatta taaagaaact
    13981 ataagtaagc tattacctac aataaaaagt aaggggttca ttttaattga tgatgcatat
    14041 gtagtagata aatctacaaa tgaacagctt aattttgaga gggtgtatcc cacttacaat
    14101 gagtggtgta tattgttcaa agaattaggt ctgaagctaa ttgaatgtat accatgttta
    14161 gaacttgaaa atgatgttga agaggaaatg aattggatta taactcgtgc aaatgagcta
    14221 attaaaaaat atccttctca aaaagatatg tttgaacaat atgttattag ccaaaaaaat
    14281 gaatatactg atttacagca ggaccttgtt ggagcaactt ggattttaca aaaagaataa
    14341 gataaatatt agactagata atcaatgtta tagtgaatta tcccatgtct agtgaatata
    14401 aataacataa aaaaccgtca agatgaatag tttattcgtg cttgacggtt tttagtattt
    14461 gttgaagtaa aattttattt ctttagtata tcacttttag tcaatccatt tgatttagct
    14521 tgagtattcc attgatgatt ccccataagt ttatatagga atctagggaa gttaggtgaa
    14581 ataaaaatag tacttttgtt ggaatcatct atatgtatat tatcactaag tatatttttt
    14641 gaaagttctt ttaatgcaat tcctaaatct tttttaggtc ctttacccaa aggtacggat
    14701 tctagtgaac caaacatctc cccagctcca attccaatac cttgaaccca tgtaaagttg
    14761 atttttttac accaatgctc tatcatttga attgccaaat gattatgttc accttcattg
    14821 aatccacagt taactattgc atacactgga ataggttttt tattttgtgt ttttaaaaat
    14881 ttttcacatt tctctaaaaa atgaagtaaa tgagatggaa taccatctac ataaagagga
    14941 gatgcgatta ttaatctatc acaattaagt atggcattaa gagcgtcagg atacttagat
    15001 gtgctatcta ctttaaatgt aaaaacttta ttatcatttt gaataagttg ttttaattca
    15061 gataaaaaat actgtgaatt atttttattt acttttggac taccatttat cattactata
    15121 ttcatatgga ttcacctctt atatcttgta tattttcata gaaacaaaca gagtgttctt
    15181 ttatattgaa atttatagca tttccattta ctaatttcgt agcagtttct ttttcagctt
    15241 ctgtaataca atctccatag aatatagctt ttaaatttat ctgtttatca tatctgcttt
    15301 tatgacgcat catattatct tttattacaa agtatggaag aatataatta atgcttttat
    15361 caagtacgtt tttgacaaaa gggctaaacc ctccgtaaca gcatctgctt attataataa
    15421 ttccatcaga gtcatttata agtttaccca tgttgctgta ataatctttt acaacacatt
    15481 cagaaggagt tttaatccag caaccaaaac aaccaataca tttgtggatt tcttttgtgt
    15541 ttggaagtat ttttatattg tcatcaagtt tgggtaataa cttttctaat tctttgtcag
    15601 ttaaatcatg tattaaaagc ctcataaagt ccatctcctt ttattatgta tgtttacttc
    15661 taaaaattat atcttataga tttttcagta aatctttaca ccaattaata acaaaattat
    15721 aatagtcaac tccataaatc attgttgcat acatatatgt atattggtca acgcaatgat
    15781 tttcttctgc atcatttttt tgtttactta atttatttag aagtaattct acttcagtta
    15841 ttaacatttt taagtttgtt tcaattattt ctttgggtag atacttgtaa aagaatatat
    15901 taacaaggtg gtcttgcttt gttttagaaa aatttatagg tttctttaac cattcaagaa
    15961 atacttcttt accagtatta gttatggaat ataatttttt taatttgcta ccatctataa
    16021 cttcgtgata gtgtatatat ccttttgttt ctaatctttt aagtgcagga tagatacttc
    16081 caaagctggc gtcaaaaaag tacgaggtac tttctgacat tatttgtttt aagtcgtatc
    16141 cacttaattc tttttccatt aaaaatccta aaataatgta ttctaacata ttacctcctg
    16201 gtatcaatta gatatatatc gattcgatat atttattatt acagataata aatcttacgt
    16261 caagatgctt tttaagataa gctaaaaaga attaggagtt attgataaat atgcaaaaaa
    16321 attaatataa aaggtaatta aatatcaatg aagatttaaa aatagttaaa tcaaagtaag
    16381 agtctaatat ataaaattta tagtaacttt atggtttaaa tataaacttg cgtgctattg
    16441 acaaagtata aaattagcag taaaataatt gcatgagcaa atagattatt gcgaacgcaa
    16501 aaaaataagg acaatagata ttaaaggaga gaatatgaga gagctattaa aatgggtttc
    16561 cattataaat agatacaata caaattatat tgacaggaaa ttggcggata ttggaatcaa
    16621 taatagccaa tatttttatg tattacacat atgtaataat ccaggtatta cacaggacac
    16681 tatatttgag aaaattcttg tgaatcctag taatataaca agagctttgg caaatttaga
    16741 aaaagaaggc tttataacaa gagaaccagg tgtaaaggat aaaagaacat ggcatttata
    16801 tcctacagaa aaatctttag gatgttatga tgaaatacta aaaataacaa atgggtatgt
    16861 tgaggagttg ttgagttcat ttgagaaaga agagcaagag ttgtttatgt caatgttaaa
    16921 gaaagttgca tttttggcta ttgatatgaa tgaagaagca aaattggagg cgaaggaaaa
    16981 tggaagaaaa atataaggag ccattggaat atgaaaaggt ttcaaagtta ttaactaagt
    17041 acgcaattcc tagtattata gcaatgttag taagtgcttt atataacatt gttgaccaaa
    17101 tatttattgg gcaaggtgtt ggtgtattgg gtaatgctgc tacaaatgtc acttttccac
    17161 taacaacaat atgtactgca attgcattat tgttaggaat tggaggagcg tctaagtgtt
    17221 cattggaatt aggtgctaaa aattcagaaa aagctacaaa agcagcagga aattctatat
    17281 gcttgatggc tgtttttgga ataagtttat ttatagtagt atcaatattt ttaacaccac
    17341 tattaaaatt ctttggctct acatcagaaa tactacctta tgcacacaca tatacaaaaa
    17401 ttacatcaat tggattacca tttcttataa tgtccactgg aatgagtaag ttgatacttg
    17461 cagatgggag accaaaagct tctatgtttt gtatgttaat aggagcagtt attaatacaa
    17521 ttttgaatcc attattcatt tttgttttta atatgggaat tgctgggtct gctttagcaa
    17581 ctgttattgg gcaacttata tcttttggaa taagtgttca ttatataaag aattttcaac
    17641 atattaaatt acataagaaa agttttaaat tagatagtat aatttataaa catatatttg
    17701 cgctaggttc aagctcatgt tttaatcaat tagcaatgac tgtaacacag atagttatga
    17761 ataatacatt ggcatattat ggggctaggt cagtttatgg aagtgagatt ccacttgctt
    17821 gtgtcggtat cattattaaa gtaaatatga tttttatatc tataattatt ggtatatcac
    17881 aaggtatgca accaattata gggtataatt atggggctga aaagtataat cgtgtaaaag
    17941 aagcatataa gtttgcagtg ggaagtgcaa ctattatatc tgttattgca ttcttatgtt
    18001 tccaattctt cccaagacaa attacgtcta tatttggtaa cggaaatgag acttatttta
    18061 tatttgcaga acattatttt agaactttct tgttcttaac atttataaat ggtgttcaaa
    18121 tagtatcatc aactttcttt acatcaattg ggaaatcagt gttgggactt tttacttctt
    18181 tgactcgtca aattttattt ttaattccat tgatagtaat tttaccaatg tttatgggaa
    18241 ttgatggagt aatgtttgca ggaccaatag cagatgcagc agcggcaatg gtatgtatat
    18301 actttgcagt tcatgaaatg aaatcattga ctataaagca aaataatttg atgagtaacc
    18361 ataataagat tgcagaggta taagacaata tttaagaagt atagaaaatt aatttaaaaa
    18421 atataataaa ttaatttagg aagtgtagaa aattaatcta aagaagtata ataaactaat
    18481 ttaggaagta tagaaaatta atctaaagaa gtgtaataaa ctaatttagg aagtatagaa
    18541 aattaattta aagaagtata ataaactaat ttaagaaata tagaaaatta atttaaagaa
    18601 atacaataca ttaattttta agtaacatta aattaatgta agtaaaataa tgttcatatg
    18661 aatatttaaa ggtgcatttt ctgaagctaa gtatatgttg tagagtgtta tgtctctcta
    18721 tcctgtgaag tttaatagct caagaaaatg cactatttat gatgattatt ttgttttatt
    18781 tccaattttg gaaataacta ttctaattaa atctaaaatt ataccataaa taagaaaaga
    18841 aagtatagat gctatgtacc aataaatcga aattgaccct gtaattgttc tacatgggat
    18901 ttgcatacct gaatccataa aaacaaaaaa actatcaact ctggaaataa tagacaaaat
    18961 aggatttaaa cctattagaa caatactttt tgagtcttga ccaatttgat gcatataaat
    19021 ctcaaataat gcaattaaaa aaaaccaaga tgtaaaagat ttaataattt ttttcatcaa
    19081 aatacctcac ataatataaa ttctgtttgc actatttgct tactacaact atttatctcc
    19141 attcagtcac atcacttaat tgtaatctag gtcttcttga tggttcttca tcaggataac
    19201 ctaaagatat tgctgccact aattgactat ctgtatttaa ccattcacat aattcacgat
    19261 atgcaaagta tacatcacaa atccaaagac tacctagacc taattccaca gctgtaagcg
    19321 acatattctg aattgcagct ccaattgatt gcatatttgc catttcatag aatcgttctt
    19381 caggggatag cttttctaaa ggtgatttac ctaatatatt taaaataaaa attgttactg
    19441 gagcttgttt catgatttca actgtgtaat ttgcaccagc gatatgttga cgacttcctg
    19501 gaagaagccc attatcatta atttcatttt gaataccttt gctcatggct ttaagcatag
    19561 attctttttc cttttcagtg atgacaacaa aacgccatgg ttgcctgttt ttagatgaag
    19621 gtgcatttat tccagcttca attattttct ctatagtttc atgtgaaatg ctttgatttt
    19681 tatattttct gatacttcgt ctttttgata tagaatcact tatcatttta agtaacctcc
    19741 gtttattttt tggaaaaatc tatttaattt ttattttcta ttttaattat tttagcatta
    19801 atcagaaaaa aagtcattga ttttagtata caattattaa tgattatatt aagaattgta
    19861 tcaaaaagta tgtgattata ctaagggatt atattaaaca ttatatttaa aattgtatga
    19921 aaggttgtat ttaatattat attgagagat atatatatta ggatttatat atattcagat
    19981 ttattattat attgagtctt atattaaatt aaggactata atttgaaaat aagctataaa
    20041 aactcaatat tgttttgttc aaagataaaa aaagaatact ttaggtgatt tttattaaaa
    20101 attaagagtg ataagattat atactttata aatgtaaata tgacggataa aaatgataaa
    20161 tataataata tatatattta cattaggagt tttctatact ataattattt aaaactaaat
    20221 ataaatgttg gaattattta ggacttgaaa ttgtctaaaa ataaatataa gtgatgaaga
    20281 ggtgatgtga tgagattaga tatggatagt atgttatatg tactaattta tatgacatta
    20341 ttggtatcta tcattggaag tatattttat ttttgtaaag acctcttaaa tcataaaatt
    20401 aagtatgttt taataagaga catatttgta aatagaatta catattctga agaacggttg
    20461 cctaaacagt atatagtttt tcagaaatat gatatttgga ggtattgtag tttgttttaa
    20521 tttgaaatga atctattata aattctaaag tttaaaaata aaaaggagag aataaaatgg
    20581 atttcatttc aagtatattg caaggattat taagtatatt atttaatttt acaggtgatt
    20641 ttggaatttc tattgtactt ataactttaa tagttaaatt agtacttatg ccactatcat
    20701 taaaacaaag attttctgta aaaaaacagc aagaattagc tgaaaaaatg gaacatataa
    20761 aagaaaagta taagaatgat actaaagaat tggaaaagca aatacaagta agttctgtag
    20821 aaagtatgaa gagtatgctg ggatgtctta tagttttggt tcaaatgcca gtagtatatg
    20881 ctttatatca tgtatgttta gatatgacaa aggaatttac aacaattata attccatgga
    20941 ttgctaattt aggtacattt gataatttgt ttatattacc ttgtatatat acattaatta
    21001 tgttagcttc aaatttagtg aattatatac catatttaaa aataaattct caagtaaagt
    21061 ttaacaaaca aatggctgtg gtgactacca taacaagtct tatactaact gttagaactc
    21121 cagtagccat aggtctgtac tttataacaa gtgcaattta ttcattaatt gaagatattt
    21181 gttttagaat atattttaat cgaaaaaata gaatagtgac aaaataatat aattattatt
    21241 agaaatattt tagcctgcta acatagaaaa agttagtagg ctatttttat ctatgatatc
    21301 aattccatta agaatagtct aaaaaatact atcaaaatgc tataaaaaat actatcaaaa
    21361 tactataaaa aaatagttga aaaataaata aattaatata aactaaaata aagcctacaa
    21421 aaaagaggtg ataaatagaa atggctaaaa tactaattgt agaagatgaa aaaaggatgc
    21481 aaagtattat tgtagaatat atgcaaaagg gaggatatac ttgtataact gctgatgatg
    21541 gagtcgaggc acttacaata ttaaagagca acaatataga tttaatgata ttggatatta
    21601 tgatgccgta tttagatgga ttttcagtgt gtagagtttc acgtgaaatg actaatatac
    21661 ctattattat attgacagct aaaggtgaag aggaggacaa attaaagggt tatgaatatg
    21721 gcgcagatga ttatataaca aaaccattta gccccaaagt acttttagca aaagttaatg
    21781 ccttgttacg tagatacact actgatattc ccaaaaattc attgagttta ggaaagattt
    21841 ttattatggt agcatcaagg caagtgtatg tagaagataa attgattgat ttaacatata
    21901 aagaatttga attattgcgt ttatttatgg aaaaccctaa tcaagttttt tccagagaga
    21961 aacttttaaa ttgtatatgg ggatatgatt ttgaaggaaa cacacgaact gtagatacac
    22021 atataaaaac cttacgcaaa aagcttggta gtgaaggaca ccatatagtt acattaattc
    22081 gttcaggata taagtttgag gttaaggaat gaatagttta agaaagatta tagaaaaact
    22141 gaaaaatgta gatatttcaa attggattaa acaaaatttt ggactatatt cagttagaaa
    22201 aaaagtgttt ttactttcta aattatcagg agcgttaatt atattatttt atctggttgt
    22261 tgaagaattt tcgataaact ctaaattaga attttggatt tggtttataa tactggttat
    22321 atttattatg agtattgatt tcttattggg aaaatttatt tcagaaccaa tcacatctat
    22381 aaataaatct gcaaaaagta tgtcccaact tgatttttct aatccatgta ctgtaaatac
    22441 taatgatgaa tttggagaac tttctaggag cctgaataca atgtctacaa atctacaaca
    22501 ggcattatca gacctagaaa gtgcaaatat tcaacttgaa aaggatgtaa ataaggaaag
    22561 aatgctattg gaacaaagaa aggagttggt agatacaatt tcacatgaaa tgaagactcc
    22621 acttggcatt atacgtgcgt atacagaggg gcttatggat gaagttgatg aagaaaaaaa
    22681 gaaaaattat atgaatgtta taatagaaga aactgataga atgaacaata tgattgtatc
    22741 attattagac ttatctgcat tggaagcagg agtttctaaa cttaacccag agcgttttga
    22801 ttttatagag tttgttgaaa ctgtggcagg tcgtctttta attgatatac cagatgccaa
    22861 tttttatttt acatatgatt tgccagagta tgaagttttt gtagtggcag ataaaatgag
    22921 aatggaacaa gtagtagaga atctagttat taatgcaaag aagcatgttg tatataatgg
    22981 taatttagat ttatcgatta catgtgaaaa tggcttgctt ttatttgaaa tttataatga
    23041 tggtaggttt atagagcaag atgagatttc aaagatttgg tctaagtttt atcgtagtgt
    23101 tcaatctcag cgtacaggag gttcaggtct agggcttgca attgtttcac aaatacttac
    23161 aatgcaaggg ttagaatatg gagtgaataa tcgtgataga ggagtagagt tttattttat
    23221 gattccaatt gatgaataat ttagtattaa tttataaatt ataagtatct gatgtaaaat
    23281 atttttcttt gtatatgtaa gcatttaaag agaataaaat aaaaaaactt aatgaaaaaa
    23341 gcttaatgaa aaagtctaat aaaaagctta atgaaaagtc ttgtttttct gatgaaaaca
    23401 agatttttta ttaagctttt tattatttca cttcgatttc acaagagaga cactctgatt
    23461 tcaccaaata gaaataaaat aggttaaata aattaaaaaa tagaagcgta attaaataag
    23521 gttacaaggg ggagtactta tgaaatggta tacatggttg actattatat tgataatatt
    23581 gctttctatt ccgcttaaaa ttaaatttat aaaatggtgg gatagacgac aaaaaaatga
    23641 taaaggaggt tatgatagat gataaaagta ggagagttaa aattttctta tggaaaagat
    23701 aaacagatat tgcatggact taattttgaa gtaaaagaag gagaaatctt tggattcctt
    23761 ggacctaatg gttcaggaaa atccacaaca cagaaaatct taaatggtgt tttaaaaggt
    23821 tacaatggtc acgtatccct ttttggtaag gaagttaaag catacacaga atctttatat
    23881 caaaagattg gtgttttatt tgaatttcct tatttatata ccaatttaag tgcaattgat
    23941 aatttagaat atttttcgtc tttttatcct aaaaatcaaa ggagagatat atatgaactc
    24001 ttagatttgt tagagtttaa gaaagagttt ataaataagc ctgtatcatc ctactcaaaa
    24061 ggtatgaagc aacgtattag tatggcaaga gcattggtaa gtaatccaaa gttgttattt
    24121 ttagatgagc ctactagtgg acttgaccca tctggagctg tactatttcg taagattatt
    24181 gaagaagaac gaaaaaaagg aacaactatc tttttgacta ctcataatat gctagatgca
    24241 gatttaatgt gtaacaatgt ggcttttatt gcagatggaa agattatggt aatagataaa
    24301 cccaaaaatt taaaaatgaa aaatagcaat aataaagttg aagtggaatt tgtatataat
    24361 ggaaatcgag atattgaaat tgtagatata caagagttag aatctggaat aacttttaaa
    24421 tatgatgaaa tcttaagtgt acattcaaaa gaacctacac tagaagaagt ttttattaaa
    24481 tacacaggaa ggatgttagt gtaatgtact ctaaattttt taccttagta aaaaaagatt
    24541 tttggatgat gatttatggt aaattttttg tgctgacact tggctctctc atactttatt
    24601 cgtgttatat taattttggt tatgtaaagt ttatggatga agatagttat cacaataatg
    24661 tatatctata taatccagag aatataagca tgagtgcatc tccattaatt aatcaagttt
    24721 cttctatagg tgaattaaaa gcaaaacttg atgaagatac aagtgcagtt tgtattgata
    24781 tgagttccag taagccgaaa gtaattttac atgaaagtat taaaggagtt gacgattata
    24841 gagctgacta tgcattgtca ttattatcat cagaaaaaga ccacaatagc aaaatatcag
    24901 acaacaatgc aataattcaa attaatgata aggatacatc tgagataaaa caaagaaaag
    24961 acatgacttg tgagatttta ttctttgaaa ttgtagctat tggattttta gggattgcct
    25021 ctttattatt taaagaaaag cagatgggag taatgcgtat tactggtatt ttacctgtaa
    25081 aaagaagtct ttttatatta tcaaagatat ttgctttttt gatatgtgac ctatgtttta
    25141 cagttctttt agtagtattt aatattggat tttttgaagg aataaaaata cttccacaag
    25201 tattagttca agctagtatt ttatcaatta ttatgtcttt gattggaata ggatgctcaa
    25261 tgttattgag agattttaag caattttctc ttatatatct tgtggttgct gtattcataa
    25321 ctacacctat atttctatct gcaaatacat cttttgaaat agattggatt aaatatcatc
    25381 ccttctatca tgtttatagt cagcttaaaa atgcgttttt catgaatttt tcaaaagaat
    25441 tagtttattt tgtagtgtgt tctttgacaa taatagttct atttttactt gtaaaatata
    25501 tttttaataa agagttggtt agggagggat aatcttgata ggattacgtt accagttaaa
    25561 gagtataaaa aaagataaaa tgtgtataat ttccttcttt ttaccagttg ttatggcgat
    25621 tttaataaat tttatgggaa caattgatat atcttcatta ggagagatac gttttggtat
    25681 agtgaaggat acacttcctg tacaaacaga gcagtggttg aagaagtatg gtatggtaag
    25741 tgtatatgac acaaaaatgc atttaatacg tgaaattaag gatattaaaa cagacacaat
    25801 tggagttgtt agagatggta aagggataaa aactgtgctt tatggaaatg agattaatgc
    25861 tataaaaaat gttggaaaga cattgccaga gatgtttaga ctaaagggtg atttaaaaga
    25921 tgttgaggtt acaatattgc ctaaagatag tattttaaaa gagtttcaaa atgtttttat
    25981 tgccatgatt ttaattactg ctatgtttat gggatgcacc tttaatgcaa tgaatattat
    26041 atctgaaaaa gaagatggaa tatcatttat aaatgaagtt ttgccacaga gtagaagtca
    26101 gtatattata caaaaagtat ttttgggtct tttatgtggg tgtttatctt caatgataac
    26161 tgtatttata tgtttggata tttcaatatt agatatgata atgatgttgt tactcgttgt
    26221 attttcagca tttatttcat cacttatagg tttatttatt ggaaagttgt cagaaggtct
    26281 tatggtagga attgtttata ttaaaatagt aatgatttta tttataggta ctcctttgtt
    26341 aatatatctt ctaggggtta ataccagtgg tatagggaat ttatactata taatgccttc
    26401 agttgctaca tttaatggta ttatggagat tattaatggt aatgtcatta ttatcaaaga
    26461 tattgtaatt ttggctgtac attgtgtagt atggtttatg atatatatta ttttagataa
    26521 aaaaagaaag ttatttttta gtagataact cagtataatt ataattggga caatttaata
    26581 ggtgatttaa tatactttgt atagtgtaat ttatgtagtg caatttgtat actttaattt
    26641 atgtagtgta atagttagtt ttattttata attattcaat ataatttagt aagaagttca
    26701 gtacaattag agctacttag tggaaaactt aataggtact taaactatat aaatacttta
    26761 ttttcctcca tttgtactat aaatttaatt tatgatataa atggaggtat tattatatga
    26821 tttgataaat ttcatcatta taacctacaa gtaaaggtat tattatataa agttaataaa
    26881 gatagtatca aaatattatt tgctataaag agaggggctc aaaataaaga taaacctatt
    26941 tttcaatgtt gaaatgataa aatatatttg atttcatata aaaaattttc ttcataaatt
    27001 aaaaagtcca ttttaagccc cctcattttt aaccagataa atcgagttaa actatttaaa
    27061 tatattactc ggattcattt gacttagata ttccttgctt gtaaaattgt tttgaatagg
    27121 ggatagccaa agcaaaaaat aaaagtataa tccctgctat gattattaaa ttattaatag
    27181 aaattacatc tgccattggt ccgaaaatca tcataccaag aggaataaaa ccagtatata
    27241 taataatagg taagctaaga actcgaccta gcatttgtgg tgctacatgc tcttgcaaca
    27301 tagtcatagc tgatgcttga atgattggaa ttgaaaaatt cgtaaaaaac ataaatataa
    27361 aaaattgcca agcttgtgtt acaagaccta gaataattga aaaaatagca taggataata
    27421 ttgctagaaa aaaggtctta tttctatttt taaaaccacc aaaaactcct ataatcaatc
    27481 cacctaaaac catacctaaa aatccaatca tttcaataat agacaggtat atataaccct
    27541 tgccaaatgt tcgttcaata agaagtccta taagaaatcc tgatggaaca caaagaagca
    27601 tgaatgctcc ataagtaaaa agcacttttc taatgaactt atctgaaaaa cagtacttta
    27661 tccctgatac catatctgca aaaaatgaga ctttttcagt ttgttttggt atatcatgtt
    27721 taggaatttt gatgaatatc aataatacaa tagcaataat tgcagtaaat acatcaatta
    27781 aaagaacttg atttatttta ttaccaaatc caagtattgc agcagctaca ataggaacta
    27841 taaattgtac tatagattgt aggctactat ttataccatt aaaacgcatt aaatgttctt
    27901 ctggtacaag ttgaggaatc attgcgttta cagcaggtgt ttgtacacca cttcctagtg
    27961 aacgtataac agatattact gatatggcta ttaattcact tgttgtgcca tttgatgaca
    28021 tcataaatag aattagaact aatgtagata tagcaattgt tgaatctgat acaattatga
    28081 gtacctttcg attgtagtag tctgcccata caccagcaaa aaaagaaatt atcatttgag
    28141 gtataaagga gcatacagtt aaaactgtta cccatacacc tgatgatgtc tgaagtgtga
    28201 cataccatat cattgccatt tgtacaagtg atgagccaaa taaggaaata tattgacttg
    28261 ctaaaaaaag aatgactttt ttcttccatg tagaattagt atttttgttt tccatcctat
    28321 tcctcctttg atttgtgtgt tgaatttttc ttgctcttaa ggataacaca caaatcaaag
    28381 tctggagtta gagtggaggg ctaggaaaat tgttttttca taatttgaag atgagatttc
    28441 atggtttttg cattatgttc agcttctttc aatgaagtta tcagtttgtc acacactgag
    28501 ataatttcgt catcatcttt tggagtgtca aggacagttt taatattagt attaggatta
    28561 gaagataaag catttaacat tcgtaaaata gctgaaagag aatagtttgc acaacgaagt
    28621 gcgcttataa ttttaagtcg ttttatatct tcatcagtat atacacgata accattttgt
    28681 tttcttttta cttttaacaa accattcatt tcccaatttc ttaaagtatc tattgtaacg
    28741 tttaagtgtt ctgcagtttc ttttctagtt aaaaagagtt tatttgtttc tggagtattt
    28801 ccatttaaaa tctgtgcaac aatagtaagt gcttcctcgg cattagattt ttctatttct
    28861 aattgttgca agtattgatt tacaagacta atagccttat caaagtccct attcgctgat
    28921 gttcttacaa tctcaagggc atcttttctt aatccccttt gtaccacttc aattttcaag
    28981 gctgtacgag caaatctaaa ttgttctata tgaaaatcat taaagatacg ataaccattg
    29041 gtattacgct taggtttagg tattaagttt agttcttcat ataaacgtac tgtattagga
    29101 tgtacaccaa ttaaatctgc tacctctgat gtcgtgtaag tgtccattag tattcaactc
    29161 cttttagata tatagtatta gtcataataa caccaaacaa aagtctggtg ttataatgga
    29221 gggtttggtt atctaagagt aattaattta tgaaagagta attaatctat tatagaagta
    29281 agtatatatt tttcaataaa ctcccatcta tcacgaggtt taaacactat tttagatggt
    29341 atggctatta ctaagtcata ttgtggaata caacaaatag catttcctcc agctcccatt
    29401 gctgaatacc acaaaacacc atctttttta catagccacc acagataacc gtattcatta
    29461 ctattcattt taactgattc ttcaatccac gatttagata taatacgttt attattccaa
    29521 ataccatcat ttaaatacag aaaaccaaat cgcaacatat ctctagcact caatgtaagt
    29581 ccccagccac ctatagaata accctgtcta tcttttgccc aacccttaag attttttcca
    29641 aagaaatctt caaaaccaaa atctttcata tcataatctg gtataacttt cataccaata
    29701 ggtttaaata agtattcatt agcaaattca cgagcagatt ttccagtagt ttttgttata
    29761 atagctgaaa gtaggtgcgt tccagctgaa gaatatttga aagtaccaat ttcgcctttt
    29821 tgaccaagtt gattcaaggt atattttatc caatcttttt gtttgcaaag tctttctaaa
    29881 gactcagtca tatttttaaa agggtatggt gcagtcattg taagtaggtg acgtatagtt
    29941 actgcttttt tacatatttc agaaggacta taagtatatt cagggaaaaa atcaacaacc
    30001 ttttggtcaa catcttttat atatccttta tctattgcaa tacctattaa ggctgatata
    30061 atactttttg taactgatgc aacatgaaaa gtatcatcag cattatatcc attatagtac
    30121 ttttcaaaga caacttttcc ttttcttgca ataattattc catttatatt actatattgt
    30181 gagattatta aaggttcaag ttgtgagagt ttttctgaac tcatgccaac acttgaagga
    30241 tttacttttt caaatattaa atctaagcaa ttgtttttat tcattaaaga tacctccctg
    30301 ttatagattt tggattttct tttagagttt ttggagaacc ttctgcaatt atataacctc
    30361 ctttgtcacc accttctgga ccaagctcaa caatccagtc acaaacattt aatacatcta
    30421 tatcatgttc aacaacaatt acagaatttc cattttcaac caattcatct aataattgaa
    30481 tcaacttaga agtatcgtat agacttagac cagtagttgg ctcatccaat acataaagga
    30541 tattaccatt tcgtttttta ccaatttctt ttgcaagttt gattctttgg gattcaccac
    30601 cacttaaagt tggtgtaggt tggcctaatt ttatataccc catacctatt tgttctaata
    30661 catttaagtg tgaaacaata tttggaatat ccttgaagaa atgtacagat tcagaaacac
    30721 tcatatttaa aatatcatgt atatttttat ttttatattt aatagataaa gattcatcat
    30781 taaatctttt tccctgacat tcactgcata tatggtctat acttagatta tttcccaacc
    30841 agaccttttc atatccacta ccactacatt taggacaagc ccctttggag ttaaatgaga
    30901 aatgaccagc tgtaaatcct cgttctaacg attctggctg acctgcaaat agtgtacgta
    30961 tcttatccca tataccaata taagaagcag gagttgagtt catatttttg ccaataggag
    31021 attgagatat ctctgaatat ccagaaatat gtttagtacc aatcagtcta tctgcaatag
    31081 tttcaacaac tgcataatcg tcttcctcac ctatttcact ctctgaagaa tttatttgat
    31141 tattgatagt atggttatta aattggcttc taagaagagg aagtagggtg tctgatataa
    31201 gtgaactctt gccactacct gattttccag atattccaac catagcaccc aaaggtaatg
    31261 atacagttac atttttaaga ttatttgtat ttgcattttg aatagttata caaggtattt
    31321 tatcagtatg tgtaatgttt ttattgcttg ttctattagg cattggatat tttccagata
    31381 gatactgtcc agtgagtgat ttgtcggatt gaagtagacc ttctaaatct ccttgatata
    31441 ctaattgacc accttcaaca ccagcttttg gaccaatatc aataatatgt tctgccattt
    31501 taattgtgtt tttatcatgc tcaactacta tgaccgtatt gcctaaatct ttaagtttct
    31561 ttattgattt taagatgtct attttttctg attcatgcaa tccagctgtt ggttcatcaa
    31621 gcacataaat taatgagtcc atctctgaat cgagatgtga atttaaaaat agtctttgca
    31681 tttccccacc actcaaagtt gacatttctc ggtataagga tagatgacct aatcgtgatt
    31741 ctattaaatg ttgagttttg gataaaatat catttacaag atttgtacca aatgtgttta
    31801 aattaccttg atttattaag agtttaagga aatcatcaat ttctaaaatc gtcatttttc
    31861 caagttctcc aatatgtttt ccgtttaaaa gtactctacg tgcttcttca ccaattttaa
    31921 aaccattgca atctgaacag gcagtcatag tataaactcc atttaaatct tcgccccttt
    31981 tgtaacgatt gtgaaatacc tttgataaag aggaactttt tttaccattt tcataatgac
    32041 catagagtac ttcatttctt acttcttcag gtagttgtaa gaattgtatg tctaaataat
    32101 cattgaattt tttagaaaat actctcaaat atccaggtgt aatctttgct ctatctaaga
    32161 cttgttttag ggtatcatgc ttatcaagga ctaatttttc aatattaatc tcaaaatatg
    32221 caccatgtcc agagcacttc atacacattc catctgaaga gttgtatgag aaataacttg
    32281 gtcttaatcg ttcttcctca tatccacaat tattgcaaat tagattatag cttacaggag
    32341 tatcacacat agtacacatt atttgacctt ccactgaata caatagtgta agcatgttga
    32401 gaatattggt tcttgtgcct actgttgaac gaggattgct ctggcgaata atattttgtt
    32461 gaacagctat agtgggagct aaaccttgaa tattatcaaa cttatcttca ctatcaatac
    32521 ctgcaagtat acctaaagat tgcagatatt gtttacgccc ttcctcaaat acaatgtcaa
    32581 acataaggct agacttacct gagccactga cacctgttgc aacaacaagc ttatctttgg
    32641 ggatagaaac atctatattt ttaagattgt gtatctttgc tccattaatt tttatttctt
    32701 tcattggttc acctcatttt atcaatttta tcaaaccgta aaatagggat atattaatcc
    32761 ttaatacaag aatagattag gtgaggttat taaaaaggtt ctataggtaa atatatatcg
    32821 accaagtgtc tattttgagg gtgattgtgt gggttgttaa gatatacttc aaaagggaag
    32881 gaggcacgta gcttataatt accatttgat agccattcat tatacataaa atcccacgca
    32941 tcactatatt cagattttga aatatcaaaa tgtcccacaa gatattttcc agaaggtatt
    33001 atcatattgc caagattgtc attctctttg acaacaatgt tatctggaat ggtcatgcaa
    33061 atactagtcc ttaattgatg ctcctgtgta aattgtggat tatcatgata aatggctaat
    33121 atttgtgtgt tttcaagtcc aagtagattc tgctcacttg catatgtgaa taaccgttct
    33181 aaaagttttg ggaaagtact ggttagatta ccatatgaac cagtatacct agtgtaaata
    33241 gtattcatat tatcaagttc tagaatttca atttcacctt taacctctct agtattctta
    33301 catttaggtt ttgatatgct tctattgtac tgagaaattt tatatggttc tttgcaattc
    33361 ttgctataat tgtttctata ttttataggg cttattttat agtaattttt aaatgctctt
    33421 gagaaaactg ctgaatcagt aaagccaaag tagtgtgcaa tatcagtaac agtcatgtca
    33481 gttctatgaa taagaaaagc agttgcattt tctagtttta gcctatttac atattgtgcc
    33541 aaagtttctt tttctattgc tttgaagatt ctatgaaaat gaaattttga aaaacctgct
    33601 atattagaaa gttcatcaag agaaagaggt tcatgtaaat gttcttctat atagtcttgc
    33661 actttgcaaa tacgatttag atattctttt ttacttttag catttgtcat atatagccct
    33721 ccttattgtg taaaatgtgt atcaattaat ttatagcaat atagtaataa aattttatgt
    33781 ttttgtaatt tatgataaat aagatagcgt ttaatttttt ataattgata aaagatattt
    33841 ataattaata aaagatataa gatgccttat atttgtcttt gcatataagt atagtcttcg
    33901 taataattag tataccatag tgatttattt ttaattgtgt atttatataa atacacaact
    33961 tatgatatgg acgaattatt atgcaaataa gctgtaatta aataaattgc aatataaacc
    34021 agttgaaaca taagttaggg aaatattatc aaagttatta cttagcataa gagtatagat
    34081 ggaataaaat aagattaaaa aattacatac taaatttaat aaaattttta tattaaaaat
    34141 aata
//
LOCUS       DAYAPA010000033        33185 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.033, whole genome shotgun sequence.
ACCESSION   DAYAPA010000033 DAYAPA010000000
VERSION     DAYAPA010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 33185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33185
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.033"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(145..681)
                     /locus_tag="ACGI59_001455"
     CDS             complement(145..681)
                     /locus_tag="ACGI59_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HFG9739248.1"
                     /translation="MEYKSPVYGIIAVPIEKIESNDYNPNAVAPPEMQLLYKSIKEDG
                     YTMPIVCYYNEEKDKYIIVDGFHRYRVMLENEDIYDRENGLLPVSVIDKSLDERMAST
                     IRHNRARGSHNVDLMSKIITELCEIGKSDEWISKHLGMEVDEILRLKQITGLAYLFKD
                     KEFGNAWIPFGSDIEEEN"
     gene            complement(699..2012)
                     /locus_tag="ACGI59_001456"
     CDS             complement(699..2012)
                     /locus_tag="ACGI59_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HFG9739249.1"
                     /translation="MDKRYLESDVFEAFQERLSYLFNEFDYIYVSFSGGKDSGLLLNL
                     VLDYKRKNNISSKIGVFHQDFEAQYSLTTEYVEKVFEDNIEDIEPYWVCLPMATRTAV
                     GNYEMYWYPWDDEKEELWVREMPVKEYIINQKNNPFTYYKYKMRQESLAKQFGRWIRD
                     IHEGAKVVCLLGTRAAESLQRYCSIVNKQHGYKGKCWITKEFKNVWTASPIYDWEIED
                     IWLAHYKFDYDYNKIYDLFYKAGVAPRKMRVASPFNDSAKESLNLYRILDPDVWVKLV
                     ARVQGVNFTSIYGKTKAMGYRSVKLPYGHTWKSFTYFLLDTLPENIRNSYIKKFSTSI
                     EFWQNTGGGLSEIVIRELLRKGYKIELNGVSNHTKDKKSRVIFLDDIPDDTDDIKFTK
                     DIPSWKRMCYCILKNDHMCRFMGFGPTKEQRIRTEYIKKKYEKIH"
     gene            2367..2807
                     /locus_tag="ACGI59_001457"
     CDS             2367..2807
                     /locus_tag="ACGI59_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HFG9739250.1"
                     /translation="MYERMLDKSNKPCLSQIIEYIGKDGYARLCKLETYLKTQYNLSR
                     ELRFPFGNNYGWGYKYSHKSFHLFYAFFEKGAFTVLIQIGDKQVPAVEKTLVALTPKA
                     KELWVTRYPCGNNGGWIKYRILEDADLLDIIQYIRAKKSPPKQN"
     gene            complement(2991..3179)
                     /locus_tag="ACGI59_001458"
     CDS             complement(2991..3179)
                     /locus_tag="ACGI59_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFG9739251.1"
                     /translation="MNKNNNLVIICMFIGMILGMAIGCAIGISKGNVGITMCYGLIFG
                     MIIGICIGTIIKNSNKKE"
     gene            4201..4596
                     /locus_tag="ACGI59_001459"
     CDS             4201..4596
                     /locus_tag="ACGI59_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6110 family protein"
                     /protein_id="HFG9739252.1"
                     /translation="MNILKMKKTGIFLGGVLFGTVGVKILSSDCVKKTCTRATAGALK
                     VKDSVLETTTKIQENVEDILAEAKEINEQEDLSTSKVNVVQDTLKCEQEVLKTETIKT
                     VEDTNSNSIKCEEIKDNVIEEKNSTCTTE"
     gene            4639..6738
                     /locus_tag="ACGI59_001460"
     CDS             4639..6738
                     /locus_tag="ACGI59_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFG9739253.1"
                     /translation="MNFYIKHEIRGRIRFDLQLGKLNNKQSDTLLYYLTSINGITKAK
                     VYDRTGDAVIYYTGEREYIIREITKFSFNNSKIENLVPEHTSRELNNYYRDKLANKLL
                     VKSISKIFFPKSLRFFLIGLHSIKYFKKGLSSLLNKKIEVSVLDATAIGISIFRKDIN
                     TAGSVMFLLGIGELLEEWTRKKSIDDLAQSMSLNIEKVWLRKGDTEILIPISEIKEGD
                     LVSVTMGNMIPLDGVIVSGETMVNQASLTGESLAVNKKEGSYIYAGTVIEQGNIVMCV
                     KEKAGTTRFQKIVTMIEESEKLKSSVESKFEHLADTLVPYSFLGSILTYAITRNPIKS
                     LSILMVDFSCALKLSIPISVLSAMRECNNNNITVKGGKFLEGVACADTIVFDKTGTLT
                     KAQPTVSDIITFQNYNKEDMLRLAACLEEHFPHSIANAVVYEAEKQGLSHKEMHTEVE
                     YIIAHGISTKVDDKKVVIGSKHFVFEDEKCTIPDGEEDKYNNLSDEYSHLFMAISGKL
                     SAVICINDPLRKEAKYVISNLRECGIKKIVMMTGDSEKTAKSIASKVGVDEYYSEVLP
                     EDKANFVKSEKLKGRKVIMIGDGINDSPAISESDVGIAMSEGAEIAREISDITISADN
                     LNNLIVLKQISNKLMKRVDLSSKFIIGFNLGLILLGVGGFVRPSTSAFLHNTSTVGIS
                     LNSMTNLLENTNTYNYH"
     gene            complement(6963..12173)
                     /locus_tag="ACGI59_001461"
     CDS             complement(6963..12173)
                     /locus_tag="ACGI59_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HFG9739254.1"
                     /translation="MANKLYSEIVNLLEEGRDELRKYDLKEKSILLFLGASGVGKSTC
                     INYLKGCVMEEKTDEETGQIYITAKDSAVEIGNGVYSKTLCPEVVDIANRDFSLCDCP
                     GFFDNRGAEYMIAGAMLVRETISTSSKVKGMVVILDYQSLLDSRHTLLIETGKNLKDM
                     LGDYAKYKENFFFLINKIPKTLIGNVTEEKIRNILEKAKEDLDSQEKDYDGCKELVYL
                     LNVALSDGVSIKLCNPLDKQPEKLLDEMKNLPETFEKTDLGLMLTASCKIEIQKAVDV
                     TYKDLEKLLVPYIESVKKGMEDSISILSSINSLKDKKIQLDKMYTDLCSRTNNMKELM
                     EFVQELSYGDGVQKVNILEETRLLLELNSYCSETHIVGLSELLKSLLKNTLEDTLKDI
                     KYWIAEEEYREEEAKKLEDAEYRIEKIQSCTYIKSYEKENVLSIYIPIPKVNLSDLTK
                     IISGYQLNILSSIYMYGDCELKIDCNMEKEVFSGKNIVLLTEKLHIPEGTYSINVSGK
                     DAEKLVGESSIYDGMNGRNGNNAGTSGDIILSFKEIEKRGELKLYVDGGRGGNGQDGK
                     KGDTGITGEAGANAKIGSKKESKEFANVKGTKYYVVKAEKGKQGGTGGTGGNGGNAGL
                     GSKSGQVYLLGKAPLLKSAISKKDGNHGLAGKGGSGGAGGAGGLGGIRGIYEETIRYI
                     GTSPPMDNSDRNYYYFDRYVFNHALNQKQAADDGEKLNTLYEEHKDWTISKREDSGSA
                     GAEGKAGHSGDALKEKELLKQEYSLSIDTFFYEFFERFGFSGIKGYRNLRLLCAVGIL
                     EADILEFSHIYEIFYAVEKIEMQLNREILQKYYSFEKKNKAVLSMDDMNIKKEFYKEA
                     KEIYEYLINKLMQSDRIESFVGKNQNFSEWSMILFALEQKTAFYVQMIEESEIGGNRV
                     TDIRSLTDYLDKTLRELEQGKKDELKEGYFNTYKESILTQIESGNQQIEVLKNELENK
                     MLSVNAKMNLLIQEIIKMKEDEQEDIEELKKKQAKLEEQMKTQLILSGIQSALSLLTA
                     FSGVGQACLGAFDSAKSLKENLSAKEEEYQWTNRMVDFSDFKLDSDGMRFVLGEQLSE
                     DRKNALDKMTYNLAGVDEERRTKFDQIKEIIVKSIDDDNYADKADDIQRAKEGLSESE
                     YKEIYTKIQKKEIGMLEANYNNKQQKDKLAALEKSKQAIERQQKTSKIKNALSIGSSA
                     CSLINTAISGVNNCLNVKSSFESKIEVVKEAIKDKENNIKKLDELNTQMVEFKDKTLK
                     ESVIPFVEKFDSTVKNQDIFENEISKLKMKEVLTELSTKFGSLKMENSEEISIIFKKV
                     QDMMETQIIITDKIDKKKDNLAMGELIYQMSDSGNQMNEYQKKLIHQIELNQVRYLCQ
                     VEAAAFQLWTFPFGSNIVNILNRCHVGITEDENEVINQAKEQNRQIREFLDQDAYQWQ
                     KTDNHILKVALTGSLDGKSRVGFENTYSLYDCNEEEVKKLLTGDEISFEVPLNKNFDA
                     IKYVTMYANIPNLRRNILEDSLKVKVHIHLYDSGYFSLYDDKKDVYYQYVFPQNYINI
                     THGLDIKYNDSDSINSIWSSVEDKGIEKFKNAGLNSGRSPYSMAKISLKAHFNKKLSY
                     DSLTNEQKKKLESYFDKPSKYAWSDSIDILRSLGYIDLKGILSKSWYEIKKDIDSDGE
                     IMKHFSILQVDLLERIIYEQTSILKENIPNLTIELVGCGIYVESEFNYLKQNDLKCYE
                     ERLVENTIIG"
     gene            complement(12188..14683)
                     /locus_tag="ACGI59_001462"
     CDS             complement(12188..14683)
                     /locus_tag="ACGI59_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFG9739255.1"
                     /translation="MPENNNQLLEEITLLLNEGKVAINKAENQNLILILGGTGCGKST
                     AINYLAGCVMMEVEDEDTGISYIDCLDPVAEIGSGAVSQTLYPNIVNMRKPIEGMDAK
                     LCDTAGFSDNRGVAHDISAAVSLGEVFKNSASICAVIVLIPEADILDSRLTKVLDLFQ
                     QLNMCLHKENFKGSITFFISKSFLGRTEKQIYNVIKRKYEQLCENLDSNIEEKAWLFE
                     ELLKDNGKNIHLCKPLASDEREGLLKQVLELDEVKDKTSAFQYPISADARLVLDTLIK
                     EIQNNIVSSLEDYVKAYTEDFINIIGSITMIQEIAKYDEVVKVFKNWLESTTSLTIES
                     FINVLAKQSGLFLQDSCMKLQNKIKGNLDIIDALSKYTEHGTGEILLPSVHMEFIIEK
                     ASKKIMEQNFIIIRKNLEKVLVSYEVQKQVFTLPNREKLMEVANKKDYVYSVSDFNSI
                     LTNVTVTQNEELEDSITRLSAYGEEGNEIIREFIGKYILNPFIVKTININDKKGIEIR
                     ALIPNLVISSVLEYFKNRKVDDNVSTFMFFAKDTIYMDDNLGMNFASEKNIILASNNL
                     DVGSNVKINVSGKSGELVDNSMYGKNGEDGRDAGNIYLYIKEEIRNYRLTLIANGGNG
                     SKGIDGENGETGASGSNGADAGYTSYFHGQMFKGYFEKGTSGKKGEKGGAGGNAGRGG
                     NAGKKGNIYFISDSEEQKNRIINHCDITVKDGEAGPNGRPGLGGYGGSGGYHGYNTLS
                     FSPSGIDKTVYYTGYFTSWDYKLDSIIKYHIEFSGGSWNEGKTGPAREYAEYGDRGDN
                     GGFNAPNTKLSTAVSKVEENLIKEKYQGYCSEI"
     gene            complement(15344..15631)
                     /locus_tag="ACGI59_001463"
     CDS             complement(15344..15631)
                     /locus_tag="ACGI59_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9739256.1"
                     /translation="MRLNITKQNTIEVENSRKEDVYFVIVDNVFFERQGDVFNSITNN
                     NLNNYDVKILPTDNIFLELKITSTKDLEPVFIVVEDRQYNHGSIMFKPVLV"
     gene            complement(15651..15926)
                     /locus_tag="ACGI59_001464"
     CDS             complement(15651..15926)
                     /locus_tag="ACGI59_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9739257.1"
                     /translation="MSLFVGGWSKFRAVEEEDLKVFNEAVGMLEGVGYEPLIVSTQVV
                     AGTNFKFICNATSVTNPPQNYLAEIVVFKPLPCDEHNATITSINIIK"
     gene            complement(16581..16760)
                     /locus_tag="ACGI59_001465"
     CDS             complement(16581..16760)
                     /locus_tag="ACGI59_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6440 family protein"
                     /protein_id="HFG9739258.1"
                     /translation="MGKDRFEKVYSQGKINATEIWVDKETGVNYVFHYYGNAAGFTPL
                     LDREGKPVISPIVSK"
     gene            complement(17045..18520)
                     /locus_tag="ACGI59_001466"
     CDS             complement(17045..18520)
                     /locus_tag="ACGI59_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFG9739259.1"
                     /translation="MKRKPQYLIIFEHIIQQIYTGKITIGDRLDSIKSMSEEYMVSKN
                     TIKTVIKMLSEKGFIETKAGSRPVLIQSTSKQSIESDLLYEKILQISEIYKIFSLIFP
                     SIAMYNIKRFTSKDFEELNEIIDCMEKNSSNYLVFKEQRLKYINKLISKTNNFLIHHF
                     CEIIQHDVIISQLAYNFDNLDNLDNCCIDFFRNKYIEQVRKTYLYGLDGNLIEFKKLL
                     TYMYKACRESILKMVENRAPQSIDTSSFNSVTLEKCYLYDLIVSDVICQIFEGNIKIG
                     DCLPSITSIINRYNVSLPTVRSAYNELNELGIAKTINGKGTYVTLFSEYDDDYINTEE
                     GAKRLSLLLDAMEFVTITLEDVVLLLGNKMDLIAVDKIETHLRYLQNNCEECKVYPDF
                     VLLCKIVDYTQIYVLQETFLHLKQYMIFGIYLERFFHEEYNEILKTRFDVCFEILRYL
                     KKGDVERFAESFSNIFKESLQYLKKVISSLNVDMRLKLGIL"
     gene            19551..20729
                     /locus_tag="ACGI59_001467"
     CDS             19551..20729
                     /locus_tag="ACGI59_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFG9739260.1"
                     /translation="MKYHIFLIITTILIIELCNIYICKIASFGNKNITIHKNYKYIFL
                     IKPLTFLIIIFTKLPLPLYYILFYIVSHKLLLSRKSSTNNHFIPNMAVISFCILHLIT
                     VSTISLVKNTSPDIIFANMTYKLISILIASILDFILWYSMNKIKEHIYIITQNQIEFS
                     LFLKFIWFTLGYILFDSIQITFAPPSSLTSYFILGSNILLILQLIIFINHIYSIEKNI
                     YLEKEHNRLKMIKQEQLLKVNLLKKKANIDELTSAYNRKFAFEKIQHYIDNNLLFSLI
                     YIDLDKLKTINDTYGHLAGDNYIINFSSIVIENLGIDDIFARIGGDEFIVIMPKSNEN
                     TTKEFIEKISKIMDNTISFSYGIVEVSSENKMTVEEIINIADIRMYTNKQNNHKRNNN
                     "
     gene            21061..21960
                     /locus_tag="ACGI59_001468"
     CDS             21061..21960
                     /locus_tag="ACGI59_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020397798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFG9739261.1"
                     /translation="MSIVLNMPAYLFDSNSIANIYRVFPISFSFIVTGLYFSYKNNKN
                     NKINFEAVLEDKKNLIFLLSLMFGMYLYLCLSLSIYYTVGNVLILKLYGIKSAIIVLV
                     GNYIAERYSFRITQIKGYDIKNQIEYVESVKTEYEINKLNDIVYTDLLTGFYNRPFVN
                     QKLSEWTSNHYKFTLCFTDLNSLKPVNDKFGHQFGDKYISTTAQIIKKLINVENSLLF
                     RYGGDEFLFLLQNTSCVEAEKIMININYELSKLSNTDEYPYALSISYGLVEWDGISDI
                     ETLISKADSLMYENKLKNKSTLN"
     gene            22169..22318
                     /locus_tag="ACGI59_001469"
     CDS             22169..22318
                     /locus_tag="ACGI59_001469"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFG9739262.1"
                     /translation="MTTVAEGIETSFQKDFLQEINCDMLQGYVFSRPLPIKDFEKLMF
                     QNSSN"
     gene            complement(22578..25145)
                     /locus_tag="ACGI59_001470"
     CDS             complement(22578..25145)
                     /locus_tag="ACGI59_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002602373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HFG9739263.1"
                     /translation="MIYYVLPLEHKQATIEIVGGKGMSLSKLLTAGIPVPDGFHVTTA
                     SYQIFVETNHIQSRINKLLDGIDSNNTSQLEDVSKKIGELFHNGEMPQEVSDAIKMAY
                     AGLGNISVAVRSSATAEDLPDASFAGQQETYLNIQGEDKVIDSVKRCWASLWTARAIA
                     YRVKNNIKHEIVALAVVVQKLAFSDSSGIMFTLNPINGRRSEMIINAAWGLGEAVVSS
                     LVTPDTIVVDKDSERIISYEVANKEIMTVRTSEGTEETMVPERLRKKYALTRNQVMQL
                     IQLGKKIEKYYQMPMDVEWALEKDKLYIVQARPITVLPPEWVLPEQDVVYTKGSLAEH
                     LPNPVTPLFATLGLEIVNRASELLWIDMFGKSAKKLLPKNGAYTVINGYVYLSAKSKP
                     LLIAIKSLSPRSLRRTLMNSVARWETARKGYEDAIKQWEEKPLHTMNAHQIMEGIQTI
                     FYAACIYFTRIQLTLPAASISETLFTKLFQGAVRRAGITDTSVFLLGFDTIVLQSEKE
                     LWGISEWVKQNNSINLYLQNSPAIKIAEDFMSSVVPVGVSQEVWIEWKNRINKYFKEF
                     GRTAYEFDFAHSTPQETLTPTFESVKTFVEGKGESPFLRQTTFEKRRKHAEEEILQHI
                     GGLRKKLFFKLLHWAQETSPMRENAIYLMGMGHPLIRCMFQEISERFIRGGAISHLDD
                     IYWLTKSELEVLIEQLDKNKSLSDMNGIIPVRKAELKKYMGYMSPSKLPEKNKKTISQ
                     AQQKQKDGKIVLTGIGTSTGVVTAPACVLNSPADFESFQPGSVLVAVTTTPAWTPLFS
                     SASAIVTDIGGPLSHSSIVAREYGIPAVMATHIATRTIKSGQMITVDGLAGTVTFDE"
     gene            complement(25148..25753)
                     /locus_tag="ACGI59_001471"
     CDS             complement(25148..25753)
                     /locus_tag="ACGI59_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFG9739264.1"
                     /translation="MPKGIVLTPEQQAERREKIVAVALSLMAENGFQKTSMREIAVLA
                     NMGKSSLYDFFKTKDEIVVYAVEKEIEKTIQQVHRIIDGEPSPQQCLRKIMLNHLRFP
                     EQYRTVLMWLNTESDYLEEEYRKRLKDARYAYQDIIKSVIDNGVTTGIFRKTDTALVT
                     RLLINSMLSISYTSRPLDSSEKMLDETMNIFLNGIMNDGGE"
     gene            complement(26127..27587)
                     /locus_tag="ACGI59_001472"
     CDS             complement(26127..27587)
                     /locus_tag="ACGI59_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HFG9739265.1"
                     /translation="MFNELKTKENVYKNLINGKWVESNSRKPIEIYSPIDNSLVGKVQ
                     SMTKHEVDEVIKNTKESIKVWAEMPVYKRGNIFHKAADLLLENIDEIANILVLEIAKD
                     IKSARAEVERTADFLRYTADVGKNMEGEAISGDNFPGGTRNKMSYVSRVPLGTVLAIS
                     PFNYPVNLSMSKIAPALIGGNAVVLKPATQGAISALHVVEIMRKAGIPDGVLNTITGR
                     GSEIGDYVVTHKGINFINFTGSTEVGQHISKISGMVPLLLELGGKDAAIVLEDADLDF
                     AAKNIVSGAYSYSGQRCTAVKRILVQESVADKLVGKIKPLVEKLTIGNPMDEVVITPL
                     IDNKATDFVQGLVDDALHKGAKLITGNVRKNNLFYPTLLDNVNVDMKIAWEEPFGPVL
                     PIIRVKDINQAIEIANQSEYGLQSSVFTSDIDKAFYIADKLEVGTVQINNKTERGPDH
                     FPFLGVKASGMGTQGVKYSIEAMTRPKAVVVNVREL"
     gene            27933..28511
                     /locus_tag="ACGI59_001473"
     CDS             27933..28511
                     /locus_tag="ACGI59_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFG9739266.1"
                     /translation="MAKSFTEIEKDNIRKRLISECEINWSKYGYKKTNIDELCSKSGI
                     AKGSFYTFFDSKEKLFFEVLNGIQNRLISSVDEILSNMPPKEGFAKSLKMLYRLYDKN
                     PFLYSPNNPDFISLINKLPQDMFLQLESNNIQSFYDLIERHNLKLKIEKEKVFGVCNA
                     LLSSLLVKENIEYNYIEVFDFMVDNLVEHILE"
     gene            28802..30052
                     /locus_tag="ACGI59_001474"
     CDS             28802..30052
                     /locus_tag="ACGI59_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFG9739267.1"
                     /translation="MVKSSNTFNKNFNLMILGQIISLFGASILKFALSLYILDITGKA
                     EIFATILAVSSIPIIVFSPIGGAIADIFNRRNLMVIFDFSSSLTVLILAVFLFNNNGS
                     ILLVGIIMTILSVISTMYQPTVQSSTPLLVDNEHLMNANGIVAGVASLTNIAGPVLGG
                     VLYGVIGINAIIVISCVSFFLSAIMEIFIQIPFIKQEQNSNIIKTIFTDIKDGIVHIC
                     KENRFILKILFLAIAINLFLSSMLIVGIPYIVKITMGASNFLYGLAEGAISFSSLVAA
                     VSIGIFSKHLKITNLHISFIICAVMFIPMAVSVYPFILNTGFPVPFLIFIVSSMLIFF
                     TTTLISIYVITVTQLKTPNELLGKVMSILFAGTAIAMPLGQVMYGKLFDLLSDRVYII
                     ILGVGFITLLIAFVSKITLRNENL"
     gene            30213..30782
                     /locus_tag="ACGI59_001475"
     CDS             30213..30782
                     /locus_tag="ACGI59_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFG9739268.1"
                     /translation="MSYMPTLEQAWEILRKYNKEEFHIRHAQIVSGVMRYYAKEYDPE
                     RVEFWEIVGLLHDLDFEMYPDEHCVKQRELMTELDLDKSIIDSTISHGYGLTGSDVKP
                     EQFMEKVLFAVDELTGLIGAAAIMRPSKSVSDLELKSVKKKFKDKSFAAGCSRDVIRS
                     GAEMLGWELDKLIQTTIEAMRSLIPEMAI"
     gene            complement(31360..32700)
                     /locus_tag="ACGI59_001476"
     CDS             complement(31360..32700)
                     /locus_tag="ACGI59_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFG9739269.1"
                     /translation="MIKSSLFWNIFIAISFMIEWFLCKKMLDYTSEKRISNKKINFCM
                     VGIIVFADIIYFFDVPPNIRVIISMIITIAFYKISYDVGFIKSVLISLIYWMLLIATD
                     ALSISLTVWLNSINDMSSMGTGDIYRIESIILGKILLIVSLLLYRALKIKLNIEINKR
                     TLFYIVIPIVANIASFFVIFEYIFNPGSGSLIHNFQFVMISVVLLISSITLVLIAKKL
                     QQYNTLVVENNMIKNTLKYENEYYEDIEKKYLKTRLLSHDMKNHILCINAMIKKGIDV
                     TSYLNNLQVEIQSNDLLFNTGNFILDAILSEKKEVCDKNDIELKIGINFSCCEAVELV
                     DICHIFSNILDNAIEACNKIDSGNRVICVKGDVMQNFYLIRVENTKINKIITKNGDIL
                     TDKKGSLHGLGIKSVKNSVNKYGGNVVIEYDDKKFVVKIAIPLRNVKKAVNHVI"
BASE COUNT        11305 a         5617 c         3885 g        12378 t
ORIGIN      
        1 agtataatta taaaaataaa aaaagaatag gataattttc ctactctttt ttctgtactt
       61 acatataata ttaaaactct ttatttttta atatatattt taaattttct tttttaataa
      121 atctttttct tttaataaat ctatttaatt ttcttcttct atatcagagc caaagggaat
      181 ccatgcatta ccaaactctt tatctttaaa taaataagct aatccagtta tttgcttcaa
      241 tctaagaatt tcatcaactt ccatacccag gtgctttgat atccattcat cagattttcc
      301 aatttcacaa agttcagtta taattttact cattaaatcc acattatgag aacctcttgc
      361 acgattgtgt cgaatagtac ttgccattct ttcatcaaga gatttatcaa ttactgaaac
      421 aggaagaagt ccattttctc tatcatatat atcctcattt tcaagcatta ctctatatct
      481 atgaaaacca tctacaataa tatacttatc cttttcctca ttataataac agacaattgg
      541 cattgtatac ccatcttctt ttatactttt atacaacaac tgcatttctg gtggggctac
      601 tgcatttgga ttatagtcgt tagattctat cttttcgatt ggcactgcga tgattccata
      661 tacaggactt ttatattcca tagcatctta actcctatct aatgaatttt ttcatatttc
      721 ttcttaatat attccgttct aattctctgt tcttttgtag gaccaaatcc cataaatcta
      781 cacatatggt cattttttaa aatacaataa cacattcttt tccaacttgg tatatctttg
      841 gtaaatttaa tatcatccgt atcatcaggt atatcatcca aaaatatgac tctactcttt
      901 ttatcttttg tatgatttga aactccattt aattctattt tataaccttt tctcaacaat
      961 tcacgtataa ctatttctga caaaccacca cctgtattct gccaaaactc aatagatgtt
     1021 gaaaattttt taatatagct attccttata ttttcaggta aggtatcaag caaaaaatat
     1081 gtgaaacttt tccatgtgtg tccatatggc aatttaacac tacgatatcc catagctttt
     1141 gttttgccat aaatagaagt aaaattaact ccttgtactc tagcaaccaa tttcacccat
     1201 acatctgggt ccaatattct gtataaattt aaactctctt ttgctgaatc attaaaaggt
     1261 gatgccactc tcatttttct tggagctact ccagctttat agaataagtc ataaatttta
     1321 ttatagtcat agtcaaattt atagtgtgct aaccatatat cttctatttc ccaatcatat
     1381 ataggagatg ccgtccagac atttttaaat tccttagtta tccagcactt tcctttatat
     1441 ccatgctgtt tattcacaat agaacaataa cgttgtagag attctgctgc tcgtgttcct
     1501 aataagcaaa caacttttgc accttcatgt atatctctaa tccatctacc aaattgtttt
     1561 gccaaacttt cttgtctcat tttatattta taataagtaa aaggattatt tttttgattt
     1621 attatgtatt ctttgactgg catttccctt acccataact cttctttctc atcatcccat
     1681 ggataccaat acatttcata atttcctaca gcagttcttg tagccattgg taaacacacc
     1741 caatatggtt caatatcttc aatattatct tcaaatactt tttcaacata ttcagtagtc
     1801 aaactatatt gagcctcaaa atcttgatga aatactccta ttttggaact tatattattc
     1861 tttctcttat aatccaaaac taaatttagc agtaaaccac tatcttttcc tccgctaaaa
     1921 gatacataga tataatcaaa ttcattaaaa agataagaaa gtctttcttg aaaagcttca
     1981 aatacatcgg attctaaata tctcttatcc atttgaatta tcccctttca attagaagtt
     2041 tataaaacct tacaatgcaa tattattatg cttttttagc agtaatcagc ataaattata
     2101 cattatttaa atacacaaat ccaactaact acatataatt ataatgtaat tatacttttg
     2161 tattataaac tatattttat cattatttta catttttttc caatatatat aaatatcata
     2221 tttttctatt agctaaaact aattacttta tatttctacg tattagtata ataatacata
     2281 aaaacttgaa taatgcataa aaacttgaca tactgttgtc ataattactt tgctataatt
     2341 aaaagtaaaa atggaggtaa attctaatgt atgaacgtat gcttgataaa agcaataaac
     2401 cctgtttgtc ccaaataata gaatatatag gtaaagatgg atatgcacga ttgtgtaagt
     2461 tggaaactta cttgaaaact caatataatt tatcaagaga acttcgattt ccttttggaa
     2521 acaattatgg ttggggttac aaatatagcc ataaatcttt tcatttattt tatgcttttt
     2581 ttgaaaaagg tgcatttact gttctgattc agataggtga caagcaagtt ccagcagtag
     2641 aaaaaacgtt ggtagctttg acaccaaaag ctaaagaact ctgggtcact cgttatcctt
     2701 gtggcaataa tggaggctgg attaagtatc gtattttaga agatgcagac ttacttgata
     2761 ttattcaata tataagagca aaaaaatctc cacctaaaca aaattaactt ttattttttg
     2821 tgatttaaat atagtgtata taaattccat tctttccata ttaaaaaata atataaaaaa
     2881 tatctattag tataacttat ttagtatgtc ttattctact ttttacagat aattaaaaag
     2941 ttttatcata aataaatcat aactagacat cctagtaaat caaaattaat ttattctttt
     3001 ttattcgaat ttttgataat agtccctata catatgccaa ttatcattcc aaaaattaga
     3061 ccataacaca ttgtaatacc tacatttcct ttagaaatac ctatagcaca acctatagcc
     3121 attccaagta tcatgcctat aaacatacat ataataacta aattgttgtt tttattcata
     3181 gtttttctcc attcaatata ttttaaattg cctagctcgc cttcttagct taaaacaaag
     3241 aaatataaat taactttctt tcattctaat atctgcctgt ataaatgtca aacaacacat
     3301 ttcttgtcat aacctcctct ttttgaagta agtacaatct cccagacgca ataatatcaa
     3361 tttcctaaaa ccatttaagt aacttttata tttacataat atttacaatc ataaactata
     3421 ttgttattca tttatacatc ataaacactc cctcctaatc attattatac ttaaaataat
     3481 gttcatttat aataatatat ctaacgcact ttaattgaaa aaactaaaac tttgcctttc
     3541 attcccatat ataaataagt atactttttg atgctctatt acaaatcaac taaattaatt
     3601 gatataaatt atcattttct gttgaaaata ccattcgcac atgctatact taattgcata
     3661 aatggtaata aatgtcatat gattttacaa atctattaat aacaattatt acttttactg
     3721 tattttaaag tttttggact aaatatcaag aactttctaa aatacaaagt acaaaaaatt
     3781 tatataagtt ataaatttat atgaactaga taagctcaaa caatatgtta tgtataaact
     3841 gtgtagtatt cggttataaa tttatataaa ttatataagc tagataaagt attacctcaa
     3901 ctatataagc tagatatata aattgcttat ataagctaga taagaattat ttcaactata
     3961 taaactacat atataaatta catacataag ctagatatat aaactgcata tataagctat
     4021 ataagaatta tttcaaccat ataaactaca catgtaaaat tcatataaat ttactaatat
     4081 aaatcaaata aaattactaa actctctaaa ctaattttgt aaaacatact ctatttttaa
     4141 catttgttac cttatacata agtacaaaca aaatctaact aaatgaaagg ataatgtaaa
     4201 atgaatattt taaaaatgaa aaaaacaggg atatttttag gaggagtact ttttggtact
     4261 gtaggtgtta agatactttc aagtgattgt gttaaaaaaa cttgcactcg tgcaacagca
     4321 ggagcattga aagttaaaga ttctgttctt gaaacaacta cgaaaattca agaaaatgta
     4381 gaagatattt tagctgaagc aaaagaaatc aacgagcaag aagacttatc tacatctaag
     4441 gtaaacgttg tacaagatac tcttaaatgc gaacaagaag ttcttaaaac tgaaactata
     4501 aaaactgtag aagatactaa ttctaatagc attaaatgtg aagaaatcaa agataacgta
     4561 attgaagaaa aaaatagtac ttgtacaaca gaataaattt aatgattatt atgattaata
     4621 atatcaggag gaagtaaatt gaatttttat ataaaacacg aaataagagg tcgtatacgc
     4681 tttgacttac aacttggcaa attaaacaac aagcagtccg atactctctt atattattta
     4741 acttctataa atggtattac caaagcaaaa gtttacgata gaactggtga tgcagtcatt
     4801 tattacactg gtgaaagaga atacataata agagaaatca ctaaattcag ttttaacaat
     4861 agcaaaatag aaaacctagt tcctgaacat accagcaggg aattaaacaa ttattataga
     4921 gacaaattag caaataaact gctagtcaaa agtatctcca aaatattttt tccaaagtcc
     4981 ttaagatttt ttttaattgg acttcattct ataaaatatt ttaaaaaagg tctctcttcc
     5041 ctgctaaata aaaaaataga agtttctgtt ttagatgcca ctgctatagg tatttctatt
     5101 tttagaaaag atatcaatac ggctggttca gttatgtttc ttcttggtat cggtgaacta
     5161 ttagaagaat ggactagaaa aaaatctata gacgacttgg ctcaaagtat gtcactaaac
     5221 attgaaaaag tatggttaag aaaaggtgac actgaaatct taattccaat ttcagagata
     5281 aaggaaggag atttagtaag tgtaactatg ggaaatatga ttccattgga tggagttata
     5341 gtatcaggag aaacaatggt caatcaggca tcacttacag gtgaatccct tgccgttaac
     5401 aaaaaagaag gctcttacat atatgctgga actgtaatag agcaaggcaa tattgttatg
     5461 tgcgtcaagg aaaaagctgg tactactaga ttccagaaaa tagtaactat gattgaggaa
     5521 tctgaaaaac taaaatcttc tgtagagagt aaatttgaac atctagcaga tacattagtt
     5581 ccatacagct ttttaggaag tatattaacc tatgcaatta ctagaaaccc tataaaatca
     5641 ctctctatat taatggtaga tttctcttgt gcattaaaac tctctatccc tatttcagta
     5701 ctatcagcca tgagagaatg taacaataac aacattactg taaaaggtgg aaaattcctt
     5761 gaaggtgttg catgtgcaga tactattgta tttgataaga caggcacatt aacaaaagct
     5821 caaccaactg tttcagatat aattacattc caaaattata ataaggagga tatgttaaga
     5881 cttgcagctt gcttagaaga acactttcca cactccatag caaatgcagt agtttacgaa
     5941 gctgaaaaac aagggcttag tcacaaagaa atgcatacag aggtagaata tattattgct
     6001 catggcattt caacaaaagt tgatgataaa aaggttgtaa taggtagcaa acattttgta
     6061 tttgaagatg agaagtgtac tatcccagac ggtgaggagg ataagtacaa caatctatca
     6121 gatgagtatt cacacctatt tatggcaata tcaggtaaat tatcagcagt tatttgcata
     6181 aatgaccctt tacgaaaaga agcaaaatat gtaatttcaa atttaagaga atgcggaata
     6241 aaaaagattg tgatgatgac tggagatagc gaaaaaacag ctaaatctat agcaagtaaa
     6301 gttggtgttg atgaatatta ttcagaagtc ttaccagaag acaaagccaa ttttgttaaa
     6361 agtgaaaaac tcaaaggtcg caaagtaatt atgattggtg atggaatcaa tgattctcct
     6421 gctatttcag aatcagatgt cggaattgca atgagtgaag gtgctgaaat agcaagagag
     6481 atttcagata ttaccatatc agcagataat ttgaataatt taatcgtttt aaagcagatt
     6541 agtaataagc ttatgaaaag agttgattta agttctaagt ttattattgg ctttaattta
     6601 ggtctgattt tacttggtgt aggtggattt gttagaccaa gtacatctgc ctttttacac
     6661 aacacatcta ctgttggaat aagtttaaac agtatgacaa atttattgga aaacaccaat
     6721 acttataact accattaatc tccattacat aaaacatatg atataaaaaa tttataattc
     6781 atctttttaa aagaatatgt cttaaaatta gcttttttct aatcaaaatt atataaagca
     6841 tactttacaa attttaaact caacttattt ctatttttta caaaaaagga gatgattcaa
     6901 attttcattt tgaaacatcc ccttttgtaa atttctttaa aatttatata ttaattatat
     6961 tttcaaccaa taattgtatt ttcaaccaat ctttcttcat agcattttaa atcattttgc
     7021 tttagataat taaattcaga ctctacataa ataccacagc ctaccaattc aattgtcaaa
     7081 ttaggtatat tttcctttag tatactagtc tgctcataaa taattctttc caataaatca
     7141 acttgtaata tagaaaaatg cttcataatc tccccatcac tatctatatc tttcttgatt
     7201 tcataccaac tctttgacaa aattcccttc aaatcaatat accctagact cctcaatata
     7261 tctatactat cgctccatgc atacttacta ggtttatcaa agtatgattc tagtttcttc
     7321 ttctgctcat ttgtaagtga atcataagaa agcttcttat taaaatgtgc cttcaaagaa
     7381 atcttagcca tagaatatgg acttctacca gagtttaatc ctgcattttt gaacttttca
     7441 attcctttat cttctactga agaccatatt gagtttattg aatctgaatc attgtattta
     7501 atatcaagac catgtgtaat atttatatag ttttgtggaa atacatattg ataatagaca
     7561 tcttttttat catcatatag agagaagtat ccactatcat ataaatgaat atgcaccttt
     7621 acttttaaac tatcctctaa aatatttcgt ctaaggttcg ggatatttgc atacatggtt
     7681 acatatttta tggcatcaaa gtttttattt aatggaactt caaaactaat ttcatctcct
     7741 gtcaaaagct ttttcacttc ttcttcatta caatcatata gagaatatgt attttcaaat
     7801 cccacacgtg atttcccatc taaactgcct gttaatgcta ccttcaaaat atggttatct
     7861 gttttttgcc attggtaagc atcttggtca agaaactctc taatctgtct attttgctct
     7921 ttcgcctgat ttataacctc gttttcatct tctgtaatcc ctacatggca tctatttaag
     7981 atatttacta tattgcttcc aaatgggaat gtccatagtt gaaaagctgc tgcctcaacc
     8041 tgacacaaat atctaacctg atttaattca atttgatgga taagtttttt ctgatattca
     8101 ttcatctgat taccactatc tgacatttga taaatcaatt ctcccattgc aagattatct
     8161 tttttcttat caatcttatc agtaatgata atttgagttt ccatcatatc ctgtactttt
     8221 ttaaatatta tagaaatctc ctctgagttt tccattttta agcttccaaa cttcgtgctt
     8281 aattcagtta aaacttcctt catctttaat ttacttatct cattttcaaa aatatcttga
     8341 tttttaactg tactatcgaa tttttctaca aatggtataa cagattcttt caaagtttta
     8401 tctttaaatt ccaccatttg agtattcaat tcatctaatt tcttaatatt attttcttta
     8461 tctttaattg cctctttaac tacctctatt ttactctcaa aactagattt tacattaaga
     8521 caattattaa caccactaat cgcagtattt ataagagaac aagcagaact tccaatactc
     8581 aatgcatttt taattttaga tgtcttttgc tgtctttcaa tagcttgttt acttttctct
     8641 agtgcagcta acttatcttt ttgctgttta ttattgtaat ttgcctccaa catacctatt
     8701 tcttttttct ggatttttgt gtaaatttcc ttatactcgc tttcacttaa cccctctttt
     8761 gctctttgaa tatcgtcagc tttgtctgca tagttatcat catctataga cttaactata
     8821 atctccttaa tttggtcaaa tttagttctt ctttcttcat caacacctgc caaattataa
     8881 gtcattttat ctaaagcatt ttttctatct tcacttagct gttctccaag tacaaatctc
     8941 ataccatcag aatccaattt aaaatctgaa aaatcaacca tcctgttagt ccattggtat
     9001 tcctcttctt tagctgaaag attttccttc aaagattttg cactgtcaaa tgcccctaga
     9061 caagcttgac caacaccact aaatgcagtc agtaaactca atgcgctttg tattccagaa
     9121 agaattaact gtgttttcat ttgttcctca agttttgcct gtttcttttt tagttcctct
     9181 atgtcttctt gttcatcttc tttcatcttt ataatttctt gaatcaacag gttcattttt
     9241 gcattaacag aaagcatttt attctcaagt tcatttttta aaacctctat ttgttgattt
     9301 ccagattcaa tttgagttag gatactttcc ttatatgtat tgaaatatcc ttcttttaac
     9361 tcatctttct ttccttgttc caattctctt aaggtcttat ccagatagtc tgtcaaagaa
     9421 cgaatatctg taacacgatt tcctccaatt tcactttctt caatcatctg cacataaaat
     9481 gctgtcttct gctcaagtgc aaataaaatc atactccatt cgctaaaatt ctgatttttt
     9541 cctacaaaag attctattct atcagactgc atcaatttat ttataaggta ttcatagatt
     9601 tcctttgcct ctttataaaa ttctttcttt atattcatgt catccataga aagtactgct
     9661 ttatttttct tctcaaaact atagtacttc tgaagtattt ctctatttaa ttgcatctct
     9721 attttctcaa ctgcataaaa aatttcataa atatgtgaaa attctaaaat atcagcttct
     9781 aaaattccta ctgcacacaa cagtctaaga tttctataac ctttaattcc agaaaaacca
     9841 aatctctcga aaaattcata gaagaatgta tctattgaca aagaatactc ctgctttaac
     9901 aattcctttt cttttagtgc atctccagag tgaccggctt ttccctctgc tccggctgaa
     9961 cctgaatctt cacgtttgct aattgtccag tccttatgtt cttcataaag agtatttaat
    10021 ttctctccat catctgctgc ttgcttctga tttaaagcat gattaaacac atatctatca
    10081 aaataatagt agtttctatc actattatcc attggaggag aggtacctat atatcttatt
    10141 gtttcttcat atatacctct aattccacca agacctcctg caccaccagc tcctccagag
    10201 cctccttttc ctgctaaacc atgatttcca tcttttttac ttatagcact ttttaataaa
    10261 ggagcttttc caaggagata aacctgtcca gacttgcttc caagacctgc atttccacca
    10321 ttacctcctg tgcctccagt tccaccttgt tttccttttt ctgcttttac aacatagtat
    10381 tttgtacctt taacattagc aaattctttt gactcttttt tagaacctat tttagcattt
    10441 gccccagctt ctccagttat ccctgtatct cctttttttc catcttgtcc atttccacca
    10501 cgtccaccat ctacgtaaag tttaagttct cctctttttt ctatttcttt aaaagataat
    10561 atgatatcac ctgaagtacc tgcattattg ccatttcttc cattcattcc gtcgtaaata
    10621 gaactttctc caaccaattt ttctgcatcc ttaccagata cgttgattga ataagtccct
    10681 tctggtatat gtaatttttc tgtaagtagt acaatattct tacctgagaa gacttctttc
    10741 tccatattac agtcaatttt caactcacaa tctccataca tatatataga tgacaaaata
    10801 ttaagttgat aaccagatat tattttagtc aaatccgata aattcacttt aggtatagga
    10861 atatatatag aaagtacatt ttctttctca tatgatttta tatatgtaca actttgaatc
    10921 ttctctatcc tatactctgc atcttcaagc tttttggctt cttcttccct atattcttct
    10981 tctgctatcc aatatttgat atcttttaat gtatcttcta aagtattttt tagcaatgat
    11041 tttaacaact cacttagacc aacaatatga gtctcactac agtaactatt taactctaaa
    11101 agaagacgtg tttcttctaa tatattcact ttctgtacgc catcaccata tgaaagttct
    11161 tgtacaaact ccataagctc tttcatatta ttagtcctac tacataaatc tgtatacatc
    11221 ttatctaatt gaatcttttt atcttttaga ctatttatag aagatagaat ggaaatagaa
    11281 tcttccatac cttttttaac actttctata taaggaacta aaagtttttc taggtcttta
    11341 taggttacat caacagcttt ttgtatttct attttacaag aagctgtaag catcaatccc
    11401 aaatctgttt tttcaaatgt ttcaggtaga tttttcattt catccaacaa cttttctggc
    11461 tgtttatcta gtggattaca taatttaata gatacaccat ctgataatgc tacatttaat
    11521 aagtacacta attccttaca accgtcgtaa tctttctctt gactatctaa gtcttctttt
    11581 gctttttcca atatatttct tattttttct tctgttacat taccgattag tgttttaggt
    11641 attttattaa taagaaagaa gaaattttct ttatactttg cataatcacc aagcatatct
    11701 ttcaaatttt tgcctgtttc tatcaataat gtatgtctag agtccagtag ggattgatag
    11761 tctaagataa ctaccatccc tttcaccttt gaagatgtgg aaatagtttc ccttacaagc
    11821 atagcaccag caatcatata ctctgctcca cggttgtcaa aaaaacctgg gcaatcacat
    11881 aatgaaaaat ctctatttgc aatatctaca acttctggac ataaagtctt agaatataca
    11941 ccatttccaa tttcgacagc tgaatctttt gcagttatat atatttgtcc agtctcttca
    12001 tccgtttttt cttccatcac acagcctttt aaataattta tacaagtact ctttccaacg
    12061 cctgaagcac ctaggaataa caaaattgac ttttctttta agtcatattt tctaagctcg
    12121 tctcttcctt cttccaaaag gtttacgatt tcactataaa gtttatttgc catacatcca
    12181 tccctttcta tatttcggaa caatagcctt gatatttttc tttaataaga ttctcttcta
    12241 cctttgaaac agctgttgat aacttagtat ttggagcatt aaatcctcca ttgtctcctc
    12301 tatctccata ttcagcgtat tctcttgctg gtcctgtttt accttcattc cagcttccac
    12361 cactaaattc tatatgatat ttaattatac tatctaactt ataatcccaa cttgtaaaat
    12421 atcctgtata gtatacagtc ttatctatac cacttggaga aaatgaaagt gtattatatc
    12481 cgtgatagcc accacttcct ccatatccac ctaaacctgg tctaccattt ggacctgctt
    12541 ctccatcttt cactgttata tcacaatgat taattattct atttttttgt tcctctgaat
    12601 ctgaaatgaa gtaaatatta cctttcttac cagcatttcc acctcttcca gcatttccac
    12661 cagctccacc tttttcacct tttttgccac ttgtaccttt ttcaaaatac cctttaaaca
    12721 tctgtccgtg gaaataactt gtatatcctg catcagctcc attacttcca gaagctcctg
    12781 tttcaccatt ttctccatct attcctttag aaccatttcc tccatttgca atcagtgtta
    12841 gacgataatt tctaatttct tctttaatat ataagtaaat attacctgca tctctaccat
    12901 cttcaccatt tttcccatac atagaattat ctacaagttc tccgctcttt ccacttacat
    12961 taatttttac attactacct acatcaagat tgttagaagc aagaataata tttttctctg
    13021 atgcaaagtt cattccaagg ttatcatcca tataaatagt atctttagca aagaacataa
    13081 aagtagaaac attgtcatct acttttctat ttttgaaata ttccaataca ctgctaatta
    13141 ctaagtttgg aattaatgct cttatttcaa tacctttctt atcattaata ttaattgttt
    13201 ttactataaa tggatttaaa atgtatttac caataaattc tctaattatc tcattacctt
    13261 cttctccata tgcacttaat cttgttatac tgtcttctaa ctcttcattt tgtgtaactg
    13321 taacatttgt caaaattgaa ttaaaatcac ttacactata tacataatcc tttttatttg
    13381 caacttccat taatttttct ctatttggta atgtaaatac ctgtttttgc acttcataag
    13441 aaactaaaac tttttctaaa ttctttctta ttataatgaa gttttgttcc attatcttct
    13501 tacttgcctt ttctataata aattccatat gtacagaagg taataaaatt tccccagttc
    13561 catgttctgt atatttactt aaagcatcaa ttatatctaa gtttcctttt atcttatttt
    13621 gtaatttcat gcaactatct tgtaagaata atccactttg tttggctaat acgttgataa
    13681 aactctctat agtaagactt gttgtacttt ccaaccagtt tttaaatact tttacaacct
    13741 catcatattt tgctatttct tgaatcattg ttatacttcc aataatattg ataaaatctt
    13801 ctgtatacgc cttaacataa tcttctaggc tagatacgat attgttttga atttctttta
    13861 tcaacgtatc taaaactaat cttgcatctg cactaattgg atattggaat gctgatgtct
    13921 tatctttaac ttcatctaat tctagtactt gctttaataa gccttccctc tcatctgatg
    13981 caagtggctt acaaagatga atattttttc cattatcttt tagcaattct tcaaataacc
    14041 aagctttttc ctctatatta ctgtctaaat tttcgcataa ttgttcatat tttcttttta
    14101 taacattata tatttgtttt tctgttcttc ctaagaaact ttttgaaatg aaaaaagtta
    14161 tactaccctt aaaattttct ttatgaagac acatatttaa ctgttggaac aggtctagta
    14221 cttttgtaag tctagaatct aatatatctg cttctggtat caatacaatt actgcacata
    14281 tagatgcaga atttttaaat acttcaccta atgaaactgc tgcactaata tcatgggcta
    14341 ctcccctatt gtcgctaaaa cctgcagtat cacataattt tgcatccatt ccttctattg
    14401 gttttctcat atttacaatg ttcgggtata aagtttgaga aacagctcct gaaccaatct
    14461 ctgccacagg gtctaagcaa tcaatatatg atataccagt atcttcatct tctacttcca
    14521 tcattacaca tcctgcaaga tagttaattg cagtactttt gccacatcct gtcccaccaa
    14581 gaattagaat taaattttga ttttcagcct tatttattgc tacttttcct tcatttaata
    14641 aaagtgttat ttcttctaaa agttgattgt tattttctgg catttatttt cccccttggt
    14701 tatatatttt atttaaataa agtttaatcc cttaagtaga ttgcaaagtt tttagactat
    14761 atttaaattt gttttttatt ttttattttt aataaatttg cattttcact aaataattat
    14821 tcttctttgt ttattaaaat aactgtgttg tataatataa tctttatcaa cacagttatt
    14881 ttatttaatg cgcttatttt attgtatttt atgtaataca gttgtttttt atattatatg
    14941 tccattattt ttctatggtt attacatata gatttttaat tatatttgta ccattaattt
    15001 aaacattcct atactactgc ttaagtttta cacctatacc agcttgaata ttttattttt
    15061 atttgcttaa atagttcaac tatactggtt taaataggtt atctttattt gcttaaatag
    15121 ttcaactata ctggttaaat aggttatctt tatttgctta aatagttcaa ctatactgat
    15181 ttaaataggt tatctttatt tgcttaaata agttcaacta tactgattta aataggtttc
    15241 ctttagttta aataattcac ctatactggt ttaaataggt ttcctttagt ttaaataatt
    15301 cacctatacc tacttgaata gttcactttt atttgcttaa attctatacc aatactggct
    15361 taaacattat actaccatga ttgtattgtc tatcttcaac tacaataaaa acaggttcta
    15421 aatctttagt agatgttatt tttaattcta aaaaaatatt atcagttggt aatattttta
    15481 catcataatt atttaaatta ttattcgtga tactattaaa tacatcacct tgacgttcaa
    15541 aaaaaacatt atcaactatt acaaaataaa catcttcttt tctactattt tcaacctcta
    15601 tagtattttg tttagttata tttagtctca tcttccacct cctattaata ttacttaatt
    15661 atattaatac ttgtaattgt tgcattatgt tcatcacaag gaagaggttt aaaaacaact
    15721 atctctgcta aataattctg tggaggattt gtaactgaag tagcattgca aataaattta
    15781 aaatttgttc ctgctacaac ttgagtagaa acaataagtg gttcataacc aaccccttct
    15841 aacattccta cagcttcatt aaatactttt aaatcttctt cttcgacagc tctgaatttt
    15901 gaccatccac caacaaaaag actcatataa aaacctcctt tatatatata tttatttata
    15961 tatagtagta ccaattttct caatattcaa tattttctgt gtaactggtc aatgaaaatg
    16021 cataactgga taaaaatact tataaaataa cttaacttta catataaata gcttacttta
    16081 catgtaataa gattattcta catgtaataa gattattcta caatatgaaa tgcatcttgt
    16141 tttgaatata ttctaaatac aataatggat attttcaatt attataatta gaaaatatcc
    16201 attacttaac ttgttttatt taaaatcaca tagagacttt attatttttt ttattgcatg
    16261 aatagctatt tcaggtatca taaggtgata taccccctcc ttgaaaccac aaaaaacatg
    16321 acaaacttta cgaatcttgt cttaaaaaac tacaggtaca ttccttattt gtggattgga
    16381 gtagccataa caagcaaatt ttacaatttt tatgatgaca aaaatcaata aaatcgctta
    16441 gtatataaca cgaaatacta cttgttacta ttaagaatag ttagatagga agtatgcatt
    16501 atacaaataa aggaatcaat aaagaaagtt aaaacatccc tacaaattaa aaatctgtta
    16561 catttttcta tatttaaacg ttatttactt acaattggag aaataacagg ttttccttct
    16621 cggtcaagta aaggcgtaaa tccggctgca ttgccataat agtggaatac atagttcaca
    16681 cctgtttctt tatccaccca aatctccgtt gcatttattt tcccttgtga atatactttt
    16741 tcaaatctat cttttcccat tttataatcc tccatatttt tgattggtct atgccttctt
    16801 cacttaagta gaaaaaatac ataacttatt tttttaattc taatatgagc acgtaaaaat
    16861 gtcaaacagc ttatttaatt tcataacttc atgttactgt tcgtaacctt gtctgtttac
    16921 ttacactgca ttacagcacc attttatcct ttacattatt gtattgcaca cagtaaaaat
    16981 gcttacttta tgggattttt caactaaaaa aaggatttta tctccacaaa acatttacta
    17041 tttgttacaa tatacctaat tttaatctca tatcaacatt taatgaagat ataacctttt
    17101 tcaaatattg taaagactcc ttaaaaatat tagaaaaaga ttctgcaaat ctctcaacat
    17161 ctcctttttt taaataccta agtatctcaa aacatacatc aaaacgtgtt ttcaaaattt
    17221 cattatattc ttcatgaaaa aatctctcta aatagattcc aaatatcata tattgtttta
    17281 aatgcaaaaa tgtttcctgt aatacataaa tttgtgtata atctactatc ttacaaagta
    17341 aaacaaaatc tggataaacc ttacactctt cacaattatt ttgtaagtac cttaaatgag
    17401 tttctatttt atctacagct atcaaatcca ttttatttcc tagtaaaagt actacatcct
    17461 caagagtaat cgtaacaaac tccatagcat ccaaaagcaa actcaacctt ttagctcctt
    17521 cttctgtatt tatataatca tcatcatatt cagaaaaaag cgttacatat gttcccttgc
    17581 cattaattgt tttagcaatt cccaattcat ttagctcatt ataggcactt ctaactgttg
    17641 gaagtgatac attatatcta ttaattatcg aagtaattga tggtagacaa tctcctattt
    17701 taatattgcc ttcaaaaatt tgacaaatta catctgaaac aattaagtca tataaataac
    17761 atttttccaa cgttacagaa ttaaaactac ttgtatctat actttgtgga gcacgatttt
    17821 ctaccatttt aagtatggat tctctacaag ctttatacat atatgttaat agttttttaa
    17881 attcaattag attcccatcc aatccatata agtaagtttt tcttacctgc tctatgtatt
    17941 tattacgaaa aaagtctata caacaattat ccaagttatc caagttatcg aaattatagg
    18001 ccaattgtga aataatgaca tcatgctgaa taatctcaca aaaatggtga atcaggaaat
    18061 tatttgtttt acttattagt ttatttatat attttagcct ttgttcttta aagactaaat
    18121 aattagaact atttttttcc atacaatcga taatctcgtt taattcttca aagtctttag
    18181 atgtaaatct tttaatattg tacatagcaa tagaaggaaa tattaaacta aaaatcttat
    18241 atatttctga tatttgtaaa atcttctcat ataataagtc agattctatt gattgtttac
    18301 tcgtactctg gattaaaact ggtctagaac cagcttttgt ctctataaag cccttttctg
    18361 agagcatttt tattacagtt tttatagtat tttttgatac catatattct tctgacatgc
    18421 tttttataga atcaagccta tctccaatag taattttacc agtataaatt tgttgaatta
    18481 tatgctcaaa aataattaaa tattgtggtt ttctcttcat atttttcctc ttcgataaat
    18541 tagatttatt gttttttgta aggtatattt acttttattt aatagggcaa aatagttgct
    18601 tattttttcc tcataatcta taattaatat aaaccattta tatatatggt ctaagtttta
    18661 taatactgta atgcagtaaa atagcatgtt gtagctatac tacagcaaat taacatacca
    18721 ttgtagtata ttaattggaa taattttaag tacttcaaaa ttccagcaaa acatatatgt
    18781 gtttttgata ttatgtatat tcgttaatag aatagctata cttgttttac ataataaatt
    18841 atatgatact tgctatatgt aatcactata tttaatacac ataactatta tacttgctat
    18901 atgtaattac tatactttat acacatagtt atcatatatg ctatatgtaa ttactatact
    18961 ttatacacat agttatcata tatactatat ataattacta tacttggaca catagttatc
    19021 atacatgcta tatgtaatta ctacacttgg acacataact actatattta ctatatgtaa
    19081 ttatactata cttggacata tagttatcat acatactata tgtaattact atacttgaac
    19141 acataactat tatatttgct atatgtaatt atactatact tggacatata gttatcatac
    19201 atactatatg taattactat acttatacac ataattatca tacatgctat atgtaattac
    19261 tatacttata cacataacta tcatacatgc tatatgtaat tactatactt atacacataa
    19321 ctattatact ttccatacat aattactata tttggacaca tagttatcat acatactgta
    19381 tattaagata taatactttt taaattttat ataacacaga cacatgcaat aaaaaagaag
    19441 gtatccataa taagctcttt gcatgtacct aaataaaaac tgtaaaatag aatatataaa
    19501 atctaatcta ttttaagtaa atacaatttc actttaaaag aggatgtact atgaaatacc
    19561 atatattttt aatcataact actatattaa taatcgaact atgtaacatt tacatatgca
    19621 aaatagcatc ttttggaaac aaaaatataa ctatacataa gaattacaaa tatatttttt
    19681 taataaaacc attaaccttc ttgataataa tattcacaaa attaccatta ccactatatt
    19741 atatattgtt ttacatagta agtcataaat tgctcttaag cagaaaaagt tctactaaca
    19801 accattttat tcccaatatg gcagtaataa gtttttgtat tttacacctt attactgtaa
    19861 gtacaatatc acttgttaaa aatacaagtc cagatataat atttgcaaat atgacctata
    19921 agttaattag tattttaata gcttctattt tagattttat cttatggtac tctatgaata
    19981 aaataaaaga gcatatttat ataataactc aaaatcaaat agagttcagt ctatttttaa
    20041 aatttatctg gttcacttta ggatatatat tatttgatag tatacaaatt acatttgctc
    20101 caccaagttc tctaacttcc tactttatat tgggaagtaa catattatta atcttacaac
    20161 taattatatt catcaatcat atatattcca ttgaaaaaaa tatttattta gaaaaagaac
    20221 ataatagact aaaaatgata aaacaagaac aattactaaa agtaaattta ttaaagaaaa
    20281 aagctaacat tgatgaatta acatcagcat ataatcgaaa atttgctttt gaaaaaatac
    20341 agcattacat tgataacaat ttattatttt cattgatata tatcgatttg gataaattaa
    20401 agacgataaa tgatacatat ggacatttag ctggggataa ctatattatt aacttctcaa
    20461 gtatagttat tgaaaactta ggaatagatg acatatttgc tagaatagga ggagatgagt
    20521 ttatagttat tatgcctaaa tctaatgaaa acaccacaaa agaatttata gagaaaataa
    20581 gtaaaattat ggataatacg atatctttta gctatggtat tgtagaagtt tctagcgaaa
    20641 acaaaatgac tgtagaagaa attattaata ttgctgatat tagaatgtac actaataaac
    20701 aaaataatca taaaaggaac aataactaaa atgaatattt acatgagctt acttgtagtc
    20761 tcactatgtt ttttatctta ttatctctat cattataatt tttttaaata tctggacata
    20821 atgattaata actctaaaaa taaatacaca gtatatatta tatattctat atttaattat
    20881 ttaattttta ttatttgtac ttatttaaag tttcatctaa ttataaattg gacaattttt
    20941 atgttgattt tattcataca aattaaagta acttataaaa tctccacaat aaaaaacatt
    21001 ttttattcac taaactgctc catcattggt ttagcaataa atatattttt taggtcttta
    21061 atgtcaatag tgttaaatat gccagcatat ttatttgata gtaattctat tgcaaatatt
    21121 tacagagtat ttcctatatc atttagcttt atagttacag gactatattt cagttataaa
    21181 aataataaaa ataataagat taactttgag gcagttttag aggacaaaaa aaacttaatt
    21241 ttcttattga gccttatgtt tggaatgtac ttgtatctat gtttgagttt gtcaatatac
    21301 tatactgttg gaaatgtatt gattttaaaa ttatatggaa tcaagtctgc cataatagtt
    21361 ttggtaggta actatattgc agaaagatat agctttagaa ttactcaaat aaaaggttat
    21421 gatattaaaa atcaaataga atatgtagaa agtgtaaaga cagaatatga gattaataaa
    21481 ttgaatgata ttgtatatac agatttactg acaggtttct acaatagacc ttttgtgaat
    21541 caaaaactat cagaatggac atcaaatcac tacaaattta ccttatgttt tactgattta
    21601 aatagcttaa aacctgtaaa tgataaattt ggacaccaat ttggtgataa atacatatcc
    21661 actactgctc aaataataaa aaaacttata aatgtagaaa attccttact atttagatat
    21721 ggtggagatg aatttttatt ccttttacag aatacaagtt gtgtagaagc agaaaagata
    21781 atgattaata taaattatga attatcaaaa ttaagtaata ctgacgagta tccttatgct
    21841 ctttccataa gctatggatt agttgagtgg gatggaatat ccgatattga aactttaata
    21901 tcaaaggcag attctttgat gtatgaaaat aagcttaaaa ataaatctac tttaaactag
    21961 ctagttaaga tattctacaa aatatatgtt ataagagagt actaagaata tcaaaatata
    22021 tagattttgc aaatattgaa tacagtttac taatgaatgc actggatgta agtgttagta
    22081 aacacctatt agatgaacac tttactgttg tttgggctaa tgaccgttat tatgaaatgt
    22141 tcagctatac ctagctaaaa agttaaaaat gaccactgta gccgagggaa tagagaccag
    22201 ttttcaaaaa gatttcttgc aagaaataaa ctgtgatatg ttacaaggat acgtgttctc
    22261 tagaccttta ccaataaaag actttgaaaa acttatgttt caaaactcct ccaattaaca
    22321 atataaaatt gcttattata agtgatatta ttgtatcaac aaataaagga attgtctcaa
    22381 aattgatttt ttctaatcaa atttttattt tgagacattc tcattttttt gcataagaaa
    22441 atacccacca agctacggtt tttcttaccg ctgttcggtg ggtattagta gtattatgaa
    22501 atctgaaaac tttaagtatt actacttccg ctaacgcaat cacaaaggtc gaaagcttca
    22561 aagcgaaaac aggcttttta ctcatcaaac gtcaccgtac ctgccaaccc gtccaccgtt
    22621 atcatttgtc cgctcttaat agttcgtgtg gcaatgtgtg tagccataac ggctgggatg
    22681 ccatattcac gtgcgacaat gcttgaatgg ctaagaggac caccaatgtc cgttacaatc
    22741 gcacttgcag atgaaaataa tggtgtccat gctggagttg tggtaactgc aaccaaaaca
    22801 cttccaggtt gaaagctttc aaaatctgct ggactattca gtacacaagc tggagccgtc
    22861 acaacaccag tactggtacc aatgcccgtc aacacaattt tcccatcttt ctgcttctgt
    22921 tgtgcttgtg atatagtttt tttgtttttc tctggcaact tggatggtga catataaccc
    22981 atgtattttt taagttcagc ttttcgaaca ggtattatcc cgttcatatc agatagagac
    23041 ttgtttttat ccaactgctc tataagtacc tccaattccg attttgtgag ccaataaata
    23101 tcatctaaat gtgaaatagc tcctcctcgt atgaatcgtt ctgagatttc ctgaaacata
    23161 caacgaatca gaggatgtcc cattcccatc aaataaatag cattttcacg cataggagag
    23221 gtttcctgtg cccaatggag caacttaaaa aatagttttt tacgcaatcc acctatgtgt
    23281 tgtaaaatct cttcttctgc atgttttcta cgcttttcaa acgtggtctg gcgtaaaaaa
    23341 ggactttccc cttttccttc cacaaatgtt tttacggatt caaaagttgg tgtaagtgtt
    23401 tcctgcggtg tggaatgcgc aaaatcaaac tcataagcgg tacgaccaaa ctctttaaag
    23461 tacttattga ttcgattttt ccattctatc catacttcct gtgaaactcc aacaggcaca
    23521 actgaagaca tgaaatcttc tgctatcttt attgcaggac tgttttgcag ataaaggttg
    23581 atgctgttat tttgctttac ccattctgaa ataccccaca actccttttc cgattgcaga
    23641 acaatcgtat caaacccaag caggaaaacg gaagtatccg ttataccagc acgtcgaact
    23701 gctccttgaa ataattttgt aaataatgtt tcactgatag aagcagcagg caatgtaagc
    23761 tgaatccttg tgaaataaat acatgcggca taaaatattg tctgaatacc ctccataatt
    23821 tgatgggcgt tcatagtatg caggggcttt tcttcccatt gtttaatagc atcctcatat
    23881 ccttttcgtg ccgtttccca gcgtgcaacg ctgttcataa gtgtcctacg taaagagcgt
    23941 ggggaaaggg atttgatagc aatcaaaagt ggctttgatt tagcagaaag ataaacatat
    24001 ccattaataa ccgtatatgc cccgtttttt ggtaataatt ttttcgcact tttaccaaac
    24061 atatctatcc ataaaagctc tgatgcacgg tttacgattt caagaccaag tgtggcgaac
    24121 aaaggtgtaa caggatttgg caaatgttct gccaaacttc cctttgtgta tacaacatcc
    24181 tgttctggca atacccattc tggtggcaga acagtaattg gacgagcctg aacaatgtac
    24241 aatttatctt tttccagtgc ccactctaca tccataggca tttgataata cttctcaatt
    24301 ttctttccaa gctgtatcag ttgcattacc tgattacgag taagagcata tttttttctc
    24361 aacctttcag gaaccatggt ttcttctgtt ccttctgaag tacgaaccgt cataatctct
    24421 ttatttgcca cttcatacga tataattcgt tcggagtcct tatccactac gattgtatcg
    24481 ggtgtaacta agctagaaac caccgcttca ccaagccccc atgcagcgtt aataatcatt
    24541 tcgctacgtc taccattgat tgggtttaac gtaaacataa taccagatga atcggaaaat
    24601 gcaagctttt gtactacaac agcaagtgcg acaatctcgt gctttatatt attcttcaca
    24661 cgataagcaa tagcacgagc tgtccataag gaagcccagc atctctttac agagtcaata
    24721 actttatctt caccttgtat gttaagataa gtttcctgct gacctgcaaa agaagcgtca
    24781 ggtaaatctt cagcagtcgc agacgaacgg acagccaccg atatgttccc taatccagca
    24841 taagccattt taattgcgtc cgacacttcc tgtggcattt ctccattatg gaaaagctcc
    24901 cctattttct tagatacatc ttcaagttgg ctggtattat tggaatcaat accatctagc
    24961 agtttattaa ttcgagactg aatatggttc gtctcaacaa aaatctggta tgaagcagtc
    25021 gtaacatgaa aaccgtctgg cacagggatg cctgctgtca atagttttga taaagacatc
    25081 ccttttccac caacaatttc aatggttgct tgtttatgtt ccagtggcaa gacataataa
    25141 atcataatca ttcacctcca tcattcataa ttccgttcag aaatatattc attgtttcat
    25201 ccaacatttt ttccgaacta tccaatggtc gtgacgtgta gctgattgat aacatggagt
    25261 taattaacaa acgtgtcaca agagcggtat ccgttttgcg aaagatgcct gtggtcacac
    25321 cattatcaat taccgatttt ataatatctt gatatgcata acgagcatct ttaagtctct
    25381 ttcgatactc ttcttctaaa taatcagatt ctgtatttaa ccacatcagt acagttctat
    25441 actgctctgg aaatctaaga tgattaagca tgatttttct aaggcactgt tgtggtgaag
    25501 gttcaccatc aataatccta tgaacttgtt ggatagtttt ttctatttct ttctcaactg
    25561 catatacaac aatctcgtct ttcgttttaa aaaaatcgta tagacttgac tttcccatgt
    25621 tggctaaaac tgcaatttct ctcattgatg ttttttggaa tccattttca gccatgagac
    25681 tcaaagcaac agcaactatt ttttctcggc gttctgcttg ttgttcgggt gtaagcacaa
    25741 ttccttttgg catagtgcga tacctccttt tagcgaccac tggtcgtttc ttttttattt
    25801 atcatacagc aaccaacggt cacttgtcaa ggttaatttt tttcattgta tatggtactc
    25861 gagataaaaa tgttcatttt agaaattact ctatttaagt agaaataggc ttcatcatta
    25921 gaaagcaaga ctataaattt gagatattta tcatgttcaa atttcatcaa tatcaaagtt
    25981 taaaatatta ataaaaaaag aattactaag aaataacata taaagattca aaaaaggagt
    26041 tgaacttaaa attcaattac aaaaaatcaa atttaagaca actcctttat tctttatctc
    26101 tactattctt tatctctatt tatttattac aactccctaa catttaccac tactgcctta
    26161 ggtctagtca tagcctcaat tgaatactta acaccttgag ttcccatacc agatgccttt
    26221 acacctaaaa atgggaagtg gtctggacct ctttcagtct tattatttat ttgaacagta
    26281 ccaacttcaa gtttatcagc tatataaaat gccttatcaa tatcacttgt aaatactgat
    26341 gattgaagcc catactctga ttgattagca atttcaattg cttgatttat atctttaact
    26401 cttataattg gaagtactgg accaaatggt tcttcccaag caattttcat atcaacattt
    26461 acattatcta acaatgttgg atagaataaa ttattttttc taacattgcc tgttattaac
    26521 tttgctcctt tatgtaatgc atcatcaacc aacccttgaa caaaatcagt tgctttatta
    26581 tctataagag gagtgataac tacttcatcc attggatttc caattgtcaa cttctctacc
    26641 aatggcttta tttttccgac taatttatcc gccacactct cttgtactaa aattctcttt
    26701 actgccgtac atctttgtcc tgaatatgaa tacgctccag atactatatt cttagctgca
    26761 aaatctaagt ctgcatcttc taatactata gccgcatctt ttccaccaag ctccaataat
    26821 aatggtacca ttccagatat tttagatata tgctgaccaa cttcagtact accagtaaag
    26881 ttaataaaat taattccttt atgcgttaca acataatccc ctatctcact acccctacct
    26941 gtaatagtat taagtactcc atctggtatc ccagcttttc tcataatttc taccacatga
    27001 agtgcgctaa tagccccttg tgtagcaggt tttaatacga ctgcatttcc accaataagt
    27061 gctggagcaa tctttgacat tgataaattt acaggataat taaaaggaga tatagctaaa
    27121 acagtaccta atggaaccct tgaaacataa gacatcttat ttcttgttcc accagggaaa
    27181 ttatctccac taatggcttc tccctccata ttttttccca catcagctgt gtatcttaaa
    27241 aaatctgctg ttctctcaac ttctgccctt gcagatttta tgtctttggc tatttcaagt
    27301 accagtatat tagctatctc atctatattt tctaacaata aatcagcagc cttatgaaat
    27361 atattgcctc tcttgtatac aggcatttca gcccatactt ttatactctc ttttgtgttt
    27421 tttataacct catctacctc atgttttgtc atagattgta ctttcccaac aagagaatta
    27481 tctattggtg agtaaatttc tattggcttt ctcgaattag attctaccca ttttccattt
    27541 attaaatttt tatatacatt ttcttttgtt ttaagttcat taaacatatt tttattcctc
    27601 tctctgatat aactttgtgt atttaaatta tagccattta ttcgttatgt aaacatttct
    27661 ttttttcata aacgtttacc tataaaaaaa ttttaagtta acgtttttct acattcttct
    27721 attttaataa catactcgct ccaaatctat ataggtcaca ctttaatact aatatatcaa
    27781 tataccaata taccaatata ccaatatacc aatataaata ttataaacat aaatcacttg
    27841 acaaatttat aagtagacaa tattatatat ctatgactca aaatattttt aaagtcatat
    27901 atatcatata tagacagaaa ggattgataa aaatggctaa aagttttaca gaaatagaaa
    27961 aagacaatat aagaaaaaga ctaatttcag aatgtgaaat caactggtct aaatatggat
    28021 acaaaaaaac taatattgat gaactttgtt caaaatcagg aattgcaaag ggttctttct
    28081 atacattttt tgattcaaaa gaaaagttat tttttgaagt attaaatgga atccaaaaca
    28141 gacttatatc atcagtagat gaaattttaa gtaacatgcc tccaaaagaa ggctttgcaa
    28201 aatccttaaa aatgttatat cgattatatg ataaaaatcc atttttatat tcaccaaata
    28261 atcctgattt catcagccta ataaataaac tacctcaaga tatgttttta caacttgagt
    28321 ccaataacat acaaagtttt tacgacttaa ttgagagaca caatctcaag ttgaagatag
    28381 aaaaagaaaa agtttttggt gtttgtaatg ctctactatc aagtctgttg gtaaaagaaa
    28441 atattgaata taactatatt gaagtgtttg actttatggt ggataatctt gtagaacaca
    28501 tacttgaata acaatttaaa ataatttttc taagtatttt tttatactaa ataggattaa
    28561 tatgattgaa ttaatgcaaa aaataaacaa tataatttat aaaataaatg ataaactaaa
    28621 tatcacacaa aacttataga ccctagagca aacccattcc actctactct aagccatcta
    28681 taatagtttg tatatttcta ataatattag atattaaagt aagaaaaaaa ttccttataa
    28741 taattgctat tatatcaatt attgtaaatg ttttaataat cttagatagg taggtacata
    28801 tatggtaaaa tcctcgaaca cttttaataa aaacttcaac ttgatgatat taggtcaaat
    28861 aatctcattg tttggtgcat caatacttaa atttgcttta tctttataca tacttgatat
    28921 aactggtaaa gcagaaatat ttgctactat actagcagta tcttcaatcc caattatagt
    28981 attctcccct attggtggag ctattgcaga tatattcaac agaagaaact taatggttat
    29041 ttttgatttt tcaagtagtc ttacagtact aatcttagca gtattcttat tcaacaataa
    29101 tggttcaata ttacttgtag gaattattat gaccatatta tcagtcataa gcacaatgta
    29161 tcaaccaact gttcagtcaa gtacacccct tttagttgat aatgagcatt taatgaatgc
    29221 aaatggtatt gtagctggag ttgcatctct tacaaatatt gcaggacctg tattaggagg
    29281 tgtcttgtat ggtgtgattg gaattaatgc aattatagtt ataagttgtg taagtttctt
    29341 tctatctgca attatggaga tatttataca gattcctttt atcaaacaag agcaaaacag
    29401 caacattata aaaactatat ttactgatat aaaggatggt attgttcata tatgtaaaga
    29461 aaatagattt atactaaaaa tacttttttt agctatagca ataaacttat ttttaagctc
    29521 tatgttaata gttggtatcc cctatatagt aaaaatcact atgggagcta gtaacttctt
    29581 atatggtcta gcagaaggag caatttcttt tagttcactg gtagctgctg ttagtattgg
    29641 tattttctca aaacatctaa aaatcactaa tttgcatatt tcctttatta tatgtgcagt
    29701 tatgttcata ccaatggcgg tttcagtata tccatttata ttaaacacag gatttccagt
    29761 tccattttta atctttatag tatcatctat gctaatattt tttacaacga ctcttatctc
    29821 aatatatgtt ataacagtca cacaattaaa aactccaaat gaactcttag gaaaagtaat
    29881 gtcaattctt tttgcaggaa cagcaattgc aatgcccctt ggtcaggtta tgtatggtaa
    29941 actttttgat ttattatcag atagagtgta catcattata ctaggagttg gatttattac
    30001 attacttata gcatttgtat caaagataac attgaggaac gaaaatcttt agatacaaag
    30061 aatttagata taaagattta attttcacta atttctgaat ttaaatgcaa aattgtgtat
    30121 cagtatgttg acgttttaga aattgattga tataatcaat ggaaaataaa ctttataaaa
    30181 attcaaaata ttcagctaga ggagtgataa aaatgagtta catgccaact ttagaacaag
    30241 catgggaaat attaagaaaa tacaataagg aagaatttca tataagacat gctcaaattg
    30301 tatctggagt tatgagatac tatgcaaagg agtacgaccc tgaaagagta gaattttggg
    30361 aaatagttgg acttttacat gatttagatt ttgaaatgta tcctgatgaa cattgtgtta
    30421 aacaaagaga attaatgaca gagcttgatt tagataaatc aataatcgat tctacaataa
    30481 gtcatggata tggattaact ggttcagatg ttaaaccaga gcaatttatg gagaaagtat
    30541 tatttgctgt ggatgaactt actggtttaa ttggtgctgc tgcaataatg cgtccttcaa
    30601 agagcgtttc tgatttagaa ttaaaatctg ttaagaagaa atttaaagat aaatcatttg
    30661 ctgctggctg ttctcgagac gttataagaa gtggtgcaga aatgcttgga tgggaacttg
    30721 ataagcttat ccaaacaact atagaagcta tgagaagctt gattccagag atggcaatat
    30781 agctcctcca tacatatacc atagaagtta catctttaaa gagcatttct ctttttgatg
    30841 caacttcttt ttatattttg tagaataaaa agtttaatat tttaaactca ctatataata
    30901 aataatataa tgtataatag tcataaactt tctaacctta aaagtttcta ataatcaata
    30961 aacaattcta atatacaaat gtcattatat gatttaatta tatatcttca atttatatat
    31021 taaaataaat atatatatat taaattctta tacaccaaat ttttacatta aacttctatg
    31081 caccacattt atacatatta aattcttata tattaaattt ctatgcatca cttttataca
    31141 tattaaattc ctatatatta aacttttatg catcactttt atacatatta aattcttata
    31201 tattaaactt ttatgcatca cttttataca ttagttctta tatattaaac ttctatgcat
    31261 cacttttata cattaattct tatatattaa acttctatgc atcactttta tacattaatt
    31321 cttatatatt aaatttctac acatcaaatt tatatacatt taaatcacat gattaactgc
    31381 tttcttcaca tttctaagtg gaatagcaat tttaactaca aattttttat catcatattc
    31441 aattacaaca ttaccaccat acttatttac agagtttttt acacttttta ttccaagtcc
    31501 atgaagagaa ccttttttgt ctgtcaaaat atctccattt ttagtaataa tcttatttat
    31561 cttagtattt tcaactctta ttaaataaaa attttgcata acatcacctt taacacaaat
    31621 aactctattt ccactatcaa ttttattaca tgcctcaatt gcattgtcta aaatatttga
    31681 aaatatatga catatatcca ctaattcaac agcttcacaa caactaaaat ttattcctat
    31741 ttttagttct atatcattct tatcacaaac ctctttcttt tcacttaata tagcatctaa
    31801 tataaaatta ccagtattaa ataacaagtc attactctga atttctacct gcaaattatt
    31861 taaataagaa gtaacatcaa tacctttttt aatcatagca tttatacaca atatgtgatt
    31921 tttcatatca tgacttagta atctagtttt taaatatttc ttttctatat cttcataata
    31981 ctcattttca tacttcaaag tatttttaat catattgttt tctacaacta aagtattata
    32041 ttgctgtaat tttttagcaa ttaaaactaa agtgatactt gatataagca atacaacaga
    32101 aatcataacg aattggaaat tatgtattaa gctaccagac cctggattaa atatgtattc
    32161 aaaaattaca aaaaaagatg ctatattagc gactatagga ataactatat aaaataatgt
    32221 ccttttattt atttctatat taagttttat ctttaaggct ctatataaaa gcaaagacac
    32281 aatcaaaagt atctttccca atattataga ctcaattctg tatatatctc ccgtccccat
    32341 actactcatg tcatttatag aattaagcca aactgttaaa gatatactca aagcatctgt
    32401 ggcgattagt aacatccaat aaattaagga tattaataca gactttataa accccacatc
    32461 atatgagatt ttataaaatg caatagtaat tatcatagat ataattactc ttatatttgg
    32521 aggtacatca aaaaaatata ttatgtcagc aaaaacaata atacctacca tacaaaagtt
    32581 tatttttttg ttagaaatcc ttttctcact tgtataatct agcattttct tacaaagaaa
    32641 ccattctatc ataaaggata tcgcaatgaa aatattccaa aataagcttg actttatcat
    32701 aatactttct cctaacgtat tactaaatta tttttttata ttttatgtat aatttaatta
    32761 tatacgactc tacacatgta gtaaatagat tgtcaaaatt attccaaata agtccatatt
    32821 tcttttcttt tttaattata tcactaaatt tgctaaattg aattaaactt tttattgctt
    32881 tttttaataa taactatata aatttatcta taactaggta taaaatatat tatttataaa
    32941 gtttttacat tatttctata ataaaatctt ataaatatta caataaaata tttttttcca
    33001 tgatatacct tgataaaatc tctgcttctt taagcagtaa ctttgagttg aatcattata
    33061 gtgatttata acatttatag aaatgctgtt atagatttat atttaaacac tacaaatgga
    33121 aatttaacta ttataaaaaa caatcattag ataaaatgat tcttacaaca aaagcatttc
    33181 tattg
//
LOCUS       DAYAPA010000034        32694 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.034, whole genome shotgun sequence.
ACCESSION   DAYAPA010000034 DAYAPA010000000
VERSION     DAYAPA010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 32694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32694
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.034"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(57..908)
                     /locus_tag="ACGI59_001477"
     CDS             complement(57..908)
                     /locus_tag="ACGI59_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFG9739270.1"
                     /translation="MNRNNFSHEIIEPIDNLDVNFKLFDDSGSYVANHWHNSLEIIYI
                     TSGDLQINMEGYTYNLKANECMFINSGIIHSTRCIYQNTSILLQVPLSFLNKYIPDFK
                     NCYFDFKVNANDNNYKKNSSKVKLILENMREIKLSSPPAANLLFTSLLFELLFELYTN
                     FKISVGNKNMKKTVLDLSKFEPVLEYTKINYKMPISINKIAKVAHLQPEYFCRKFKQY
                     MGQTYLEYLNDVRISHIYKDLINTNDTLCSILEAHGFTNTKLFYRIFKEKFKCTPKHI
                     RKNLPKI"
     gene            1125..2291
                     /locus_tag="ACGI59_001478"
     CDS             1125..2291
                     /locus_tag="ACGI59_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HFG9739271.1"
                     /translation="MYIKGVNLGGWLVLEKWMTSSLFEGTEAEDEYYLPRQLSREAYE
                     SRIKTHRSEYITERDFAMIKSMGFNSVRIPVPYFIFGDCEPFIGCVKELDKAFAWADK
                     YGLSILIDLHTVPGSQNGFDNGGISGICSWSQNPEYVAFTLNVLERLAKRYGMRHELY
                     GIQILNEPITERMWNIMNVPNRFKAVDKEMARGSKPNSLEFLRDFYIKAYKVMRPYMR
                     EENVIVFHDAFELKAWKDFMREEEFKNVVLDTHQYLMLAEADGCEQSIDSYLKYIREN
                     YAKDILQMQKYFPVICGEWSLFNSYACGIDTNGGQSPLNGIESNIDKLSKDDKRELYR
                     KIAKAQLDAWRNGSGHYYWNYKLLLDTVNEEGWIGWDSWDLVKCVAQEWYPIEY"
     gene            2313..3959
                     /locus_tag="ACGI59_001479"
     CDS             2313..3959
                     /locus_tag="ACGI59_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HFG9739272.1"
                     /translation="MIKNPILPGFNPDPCICRKGDDYYLVVSSFEWFPGIPVYHSKDL
                     KNWELYTHILTDETKIDLKKLPSSKGIWAPCLTYCEEEDLFYIVYGIMNSMNARYFDV
                     DNYLITSKDIKGEWSEPVYLHSSGFDASIFHDDDGKKWIASLDWETREGYEKPGVICL
                     VEYCTKKKEIVGYPKRIWSGGTDRGCIEAPHITKRGDYYYIMCAEGGTGYGHSVTMGR
                     AKNIWGPYEKDSMNPIVTSIPGDFYERHDPDHLKPKYYNPESKLQKSGHGSYIETTSG
                     EVYLVHLTSRPFVPELRCTLGRETAIQKMKWTKDNWLRMEDESNLAKEYVSESKLEEH
                     LVSSIPSFDDFDSNELGLQYYAPRISPLSFADVKSRPGYVRIRGQESRTSLNKVSILA
                     RKLTSVYARITTKMEFYPEVHQHSAGLIMYYDNMNYINLRKYYSETLGQSALSIIHLE
                     NGEKTEFLNTRIPIKDIPIYLRLYVQGRKSYFEWSYDEKNYQRIGKVFDTTKFSDEYC
                     KYGEFTGTFIGLTCADRVKHKHYADFDFFEYIVDESKDVD"
     gene            4056..5429
                     /locus_tag="ACGI59_001480"
     CDS             4056..5429
                     /locus_tag="ACGI59_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFG9739273.1"
                     /translation="MNTEVNVKATTIRYEKISFFERISYGCGDLGCNIIYSAMSAFLL
                     FYYTNYADVSAAAVGSIMLVSRILDGFSDLTMGIIVDRTKSKYGKARPWILRMAIPFA
                     IAAVLLFSVPSNLGITSKLIYIFITYNLVSTVIYTAINVPYATLNSLITQNQYERGVL
                     SIFRMILATCGTLLINGLTLPLVEYFGNNLSAWTKTFFVFGIASIMVFFITFTGTKER
                     VKAVKQDRNEVIPFKIGIKSLFRNKYWIQITLCLVCIFIVFAINGGSSVYYAKFILGD
                     EKLFGPINMVSNISQIIAMFMVAPFIKKFGKRNVLIVGSIILISSNVMFIIVGQNHIG
                     VIFASAIKGIGSAGIASTMFAIVSDTIEYGEWKTGYRTEGLINSASSFGFKVGNGLGS
                     AILGAVLSIGGYVGTSATQSDLAIVSIKVCFIYLPIFITILQIIIMSFYKLDKEYSTI
                     LNELNTK"
     gene            5874..6827
                     /locus_tag="ACGI59_001481"
     CDS             5874..6827
                     /locus_tag="ACGI59_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HFG9739274.1"
                     /translation="MKKIGDLRLMMKCCSLYYEDNLNQQEIANQLGISRPTISRILKE
                     AFEQGIVKIQIVDVLKNDYQKIERSLERKYKLKEVIVVDDKQDALTQKQELARAVSEY
                     LTRVVKENDIIGVSIGTTLKEIPRYVEKSNCKNVTFIPLLGGIGDNEIDIHANQIAVS
                     LARAFGGDFKLLHAPAVMSDLSTKEKLCKDEKIKEVLDLIDKTTIAIVGIGNPMSLNS
                     TIMASGYMNEADIEDLKKYNSIGAICLQAFDKEGKTSILEFNQRVLGVKLEDLKKIKR
                     TIGVASGDEKIEAIKASLKAKFINSLAINHSLALKLLEDCD"
     gene            6865..7827
                     /locus_tag="ACGI59_001482"
     CDS             6865..7827
                     /locus_tag="ACGI59_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HFG9739275.1"
                     /translation="MSISKETLLEMYKRMNQARKFEEKVSWFFARGMVHGTTHLSVGQ
                     EASSVAAVMALEKGDLVSLTHRGHSQFIGMGIDLNKMMAELMGKETGFCKGKGGSMHI
                     ADIESGNLGANGVVGGGLTIAPGAALTQQYKKTGKIVLCSFGDGASNEGTFHEGINLS
                     SIWKLPIIFYCENNLYGMSTSIKRHMNIESIATRAASYGIEGISIDGYNPIEVYETVQ
                     KAAEKCRRGEGPVLIESRTYRWLGHSKSDANVYRTKEEIESWKAKDPIEFLKNYLIEN
                     NLSNEDELDKIQEFAKQSIEDAVEFAQNSPNPKIESLLEDVYAD"
     gene            7852..8838
                     /locus_tag="ACGI59_001483"
     CDS             7852..8838
                     /locus_tag="ACGI59_001483"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HFG9739276.1"
                     /translation="MSTRELTYAQAIKEAMSEEMRRDENVIFMGEDIGIYGGAFGVSV
                     GMIDEFGPERVRDTPISEAAIAGAAAGAAATGLRPIMEVMFMDFVTISMDAIVNQAAK
                     MRYMFGGKAQVPMVVRCPGGSGTGSAEQHSQSLEAWFCHVPGVKVVAPSTPADAKGLL
                     KAAIRDNNPVIFVENKLLYRKKGFVPEDDYVIEIGKADIKREGTDVTVITYGRMLQSV
                     EEAAETLSKENINVEIIDLRTLYPLDKETIVKSVCKTGRVLICHEAAKTGGLGGEISA
                     LITESESFDYLDAPVKRICGKDVPIPYNPELEKAVVPRVDEIEEAIKSLIVR"
     gene            8851..9897
                     /locus_tag="ACGI59_001484"
     CDS             8851..9897
                     /locus_tag="ACGI59_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HFG9739277.1"
                     /translation="MVANKIKATPAARSQARKDNIKLDRLIGSGENGRIHLVDVLNYL
                     KDNKANTTPLARRIAEDLNIDLETIVGTGYNGKIRKCDVEKLTAKETIVSTNTSKSSE
                     KKELKIENENSSMFNTVEGIFEKPNPMRATVAKRMSESYFSAPVFTFNIEVDATELKV
                     LRAKLIDTVKESTGVKLTMTDLIVMAVSKILPNHQALNSAWTDEGIFRYKDVNIAIAV
                     GLDEGLYVPVVKNANKKSLKEIAKESKELAEKVKTGKLMPADQEGNTFTISNVGMYGI
                     TTFTPIINMPSSAILGVGATQDKFVPVNGEAKIKPIMNLSLTSDHRVIDGTVAAKFLK
                     DLKELLENPLSMLV"
     gene            10064..11794
                     /gene="lpdA"
                     /locus_tag="ACGI59_001485"
     CDS             10064..11794
                     /gene="lpdA"
                     /locus_tag="ACGI59_001485"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFG9739278.1"
                     /translation="MSVEVIMPKAGVAMEEGTIVSWLKQEGEEVKIGEPILEITTDKV
                     NMEIESEGEGTLAVIIHKEEGEVLPVFTVIGVIAEKGENQEEVKAKYLSGNVSKEDIV
                     EENQNIEFKEEKINKKECNHDYDVVVIGGGPGGYLSALKAALLGGRVALVEENILGGT
                     CLNRGCIPTKTYIKTAEILEEIDQLSKRGVKVTVDKEQDIKKAIKYKNRVVKKLTAGV
                     GGLLKSRDVDVFNLKASVKEEHKVILSDGKVLDTENIIIATGSKVRILPIKGIESNLI
                     ITSTEALDLETVPEELVIIGGGVIGCEFAEIFNSRGSKVTIVEMEDRVIPRMDKELSE
                     SLKYSLSKKGINVLTKKKVSEFKEEGNNILVCIEGEEPIKADLCLYAIGREANLSGIE
                     DLDIKIDKGSIVVNSKMETSIPSIYAVGDVTGGVMLAHAAFKMGEVAASNALGVNKEV
                     DLGALPSCVYTIPEVASVGITEEDARKKYNVKVGKFNFAGNGRALASGQEQGYVKVVA
                     DAKYGEILGIHMFGCGVAELINHAASFKALEIPTDEASELIFGHPCTSEALMEALADV
                     NGECLHLPKK"
     gene            12212..15160
                     /locus_tag="ACGI59_001486"
     CDS             12212..15160
                     /locus_tag="ACGI59_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFG9739279.1"
                     /translation="MRQRIKNLIENEDKKNPLTDEAISSRLNIRREDVTFLRNELKIE
                     DSRQRRKVVLIKAIKDILKEEKTINKNDITRRLNSIGFTVSRFTVIQYLKEIQDSGDL
                     KLDKESDDKKEEIINNRENKDIAFDKLIGSSGSLSTIIKLAKAAILYPPHGLHTIIMG
                     PTGVGKSELAECMYKFAVESSRFPKNAPFIVFNAADYSENPQLLLSQLFGHVKGAFTG
                     ADENKEGLVSKADGGILFIDEIHRLPHEGQEILFQLIDKGMVRKLGETRLTHKIDVMI
                     ISATSEPIDSHLLNTFKRRIPVTIEIPELVARPLNERFDIINNFFLIEAQRMGANIHI
                     KSDVLKALMLYDCIGNVGQLRSDIQVACARGLLNQLTNKLKEVNISISDVPGYVKQGL
                     IKIRNCRGKIENYVDGDLIIDSNLQGEIEKKNEDIYTFPDEIYKLTERRHIDLLEQGL
                     DFDVINRIIGGELESCIQKYIKQVKKVSISNNLPDISPIVGKYIVELTYEVIKIASQY
                     LSSLEPNLHLCLAVHLKAAFERLKEGKVISNAHLKEIIGNYPLEYSVAEKVAQYLKER
                     YNINLPKEEIGIIAIYFKMSSRRDMSEQKKIGVLLMAHGRVASSVCEVTNKLLGINHA
                     RYLDMPFEKKPEEMIEEATTIIKDIDEGRGVIILVDMGSLSYFGDIISGKTGINIRTI
                     SRFDTLLSIEVIRRAVLPDLTIDEIVDEVKGIEPQVKEKVSKCNNTKVDFRKPIILTL
                     CITGLGGACKIKKILEENIKSITEKVDIEPIGMIEYPSLDLEIKQISNDKNVIAIVGT
                     IDPQIANIPFFSLVDINDKAKLNQINNLIDTNQIGETNKKYELSDLIHKEDIFVDLQV
                     KSKEEVLKYMSKKLREKGYVTKNFYKKVVERDNLFPTELSDEIAIPHTDGIDVIRPAI
                     SIAILKKPIVWSKSKVKIILLLAVDQKCFNPLSTLLSFIETDEFKKIKNIKDRDFIRE
                     MILDGVNENN"
     gene            15138..15602
                     /locus_tag="ACGI59_001487"
     CDS             15138..15602
                     /locus_tag="ACGI59_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFG9739280.1"
                     /translation="MESMKIISSNLIFKNIEVSNNEDALKFLGQRLFDEQYVKESYIQ
                     AVVAREKKYATGLPTEIYGVAIPHTDIVHVNEPGIAIGILNKPVKFIMMGTDDTEIDV
                     KVVFMLAVKEPQEQLQLLEKLMTIFQDKNILNNIVDLSEESVSDLLNSKLKN"
     gene            15715..16017
                     /locus_tag="ACGI59_001488"
     CDS             15715..16017
                     /locus_tag="ACGI59_001488"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFG9739281.1"
                     /translation="MGVTNVAKKILVACGTGVCTSTIAINKLKKALQDIGKLDMVNIT
                     QCKVVEVASKAPDYDLIICTTQVSSSIKTPVINGLPFLTGVGMDKLINDVLEELEL"
     gene            16094..17443
                     /locus_tag="ACGI59_001489"
     CDS             16094..17443
                     /locus_tag="ACGI59_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFG9739282.1"
                     /translation="MEILNFIVGLGAQVMMPIIICIFGLILGTKLGKSLRAGLTVGVG
                     FIGLNTIIALLTDNLGPATQQMVKNLGLSLSIIDVGWPAASAIAFASTVGALIIPIGL
                     VVNIVMLITNTTQTVDVDIWDYWHFAFTGALVAGATQSVMWGVFAAVANMVIVLVMAD
                     LTAPGIEEYLGMPGISLPHGFTQAFVPIAIVVNKLLDLIPGINKIEINADTLQKKFGL
                     FGEPLIMGSVIGVIIGIAAKYDIKGILQLGVTMGAVLILIPKMAALLMEGLLPVSEAA
                     QEFIEKRFKNRGKIYIGLDSAVGIGHPVTLSVALVLVPLTILIAAILPGNKVLPFADL
                     AVIPFALVLIVPITKGNVFRTLIIGIIIITSGLLIATNLAPLFTQMALNASFKMPEGA
                     TMISSICDGANPLSWVFVKVMNYKVIGGVVFGVIALGMAIYNRNRIINENKKLSIEE"
     gene            17478..17951
                     /locus_tag="ACGI59_001490"
     CDS             17478..17951
                     /locus_tag="ACGI59_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9739283.1"
                     /translation="MKKYKYDSKRYLLSVTIPGFFMILILIYALFNNYTNFNLNIYSL
                     LIIVCTYGIFNTFISSSNPKKIIIDSKCIHFTSFMTTHKYEIKDIEKIRIREFYNKKI
                     YLKINDGKLFKGRYWIRTNMFNNSIALYSYFIELEECLYPDSLKFRNKKFENKNI"
     gene            18123..18773
                     /locus_tag="ACGI59_001491"
     CDS             18123..18773
                     /locus_tag="ACGI59_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HFG9739284.1"
                     /translation="MLLEKLRKEVLQASLDLLNYNLVTLTGGNVSGRDEQTGYIAITP
                     SGMDYRNLTPSDIVIVDVDGNIIDGKWKASVDLSDHLYIYKHREDINSIIHTHSTYSS
                     CFAILNEPIECASTTLANEVGGSVPVAKFSPPTSKKMGKCVIEAIGDKRACLLANHGV
                     IAVGPSVGHALTAAVMLEDSAKVYYLAKSIGTPVLLPDEEIQRARDVFFNVYGQDK"
     gene            18947..19879
                     /locus_tag="ACGI59_001492"
     CDS             18947..19879
                     /locus_tag="ACGI59_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoate A/B protein ligase family
                     protein"
                     /protein_id="HFG9739285.1"
                     /translation="MYYLLNKSTNPYFNLALEEYLFLNDKYNDDIIIIWRNEESIFIG
                     KNQNPYQEVYHDVIEKGEIPILRRISGGGTVYHDLGNINMSFIQKDRQLHEIDFLEHT
                     KFMQKMLSTLGLDVSITERKDLFLNGKKISGSAQSIKRKNSLYHGTLLYDSDLNKLTK
                     YLNSNKATESNATKSVSSKVTNIKFLLEKDINYFLDYCVEYLKSNIKNIKELELDEED
                     IKSVYNIGEDKYKKIEWTYGKTPKFQVVLPFNNESKINIHVNRWKVSKFFISYDDNVI
                     NMDKLLDLNFFKEEIKESILENYPHYIDLINLIF"
     gene            20153..20866
                     /gene="ispD"
                     /locus_tag="ACGI59_001493"
     CDS             20153..20866
                     /gene="ispD"
                     /locus_tag="ACGI59_001493"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HFG9739286.1"
                     /translation="MYSVIIVAAGSGRRMNLDINKQFIKLREKEIIAHTIQVFYENIN
                     IDEIVVCIKKEEEDFFKENIINKYNFKNIKIAYGGKERQDSIYNGLKKLDKNCDIVLI
                     HDGARPFVDHRIINESIKVAKEKKAVVVGVPVSDTIKIVSDGTVQETPERNLLWAAQT
                     PQTFEYNLIIDAYEQAYKNNYYGTDDSMLVENIGQSVTMVMGSYENIKITSPEDLNIA
                     EQILNMEKRDEVNSRRRII"
     gene            20863..21348
                     /gene="ispF"
                     /locus_tag="ACGI59_001494"
     CDS             20863..21348
                     /gene="ispF"
                     /locus_tag="ACGI59_001494"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HFG9739287.1"
                     /translation="MRIGLGYDVHKLTEDRKLIIGGVEIPHDKGLLGHSDADVLIHAI
                     MDSILGALALGDIGKHFPDTDEEYKGADSMKLLEHVYNLITSKGYKIGNIDSTIIAQS
                     PKMAPYIESMRSNISKVLNTDIDNINIKATTEEGLGFTGAKQGIASQSICLLLLTSQN
                     N"
     misc_binding    21443..21651
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            21715..23430
                     /locus_tag="ACGI59_001495"
     CDS             21715..23430
                     /locus_tag="ACGI59_001495"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFG9739288.1"
                     /translation="MKMSKMFMPTLKEIPADAEITSHQLMVRSGMIKKMTSGVYNQLP
                     MGLRVFKKIEQIIREELNKKDCQEILCAALLPSELWKESGRWTAMGEEMFRLKDRTER
                     EYCLGPTHEEAFTDIIRQEITSYKQLPLNLYQIQVKYRDERRPRFGVMRTKTFTMKDA
                     YSFDVDDKGLDKSYQDMFDAYVSIFNRCGLENSPVQADSGAIGGSTSAEFMVKSEVGE
                     DEVVFCSGCDYAANVERAESCNLASQKEEMKELEEVHTPGAATIKELEEFLKTSPDKF
                     AKTLVYEADGKTVVVVVRGDREVNEIKVSNAIGSVIEFALATDDVVRKVTNAEVGFAG
                     PIGINADYVFIDKEIVEQRNIVVGANKTEYHIKNANYGRDFEGIVGDFRNVQEGDKCI
                     VCGKPLEIARGVEVGHIFKLGTKYSESMNANFIDKDGKSKPIVMGCYGIGVERTAAAI
                     IEQHNDEKGIIWPLSVAPYHVVIVPANMKNEEQISIAENIYNDLQAMGVEVLLDDRDE
                     RIGVKFNDSELIGIPMRITVGKNINEGKVEFKLRHKEDKEIIDIEEINEKVKAEFIRN
                     NVRLG"
     gene            23552..24997
                     /gene="proS"
                     /locus_tag="ACGI59_001496"
     CDS             23552..24997
                     /gene="proS"
                     /locus_tag="ACGI59_001496"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFG9739289.1"
                     /translation="MAKNEKQFVEEITKMEDDFPQWYTDVITKTDLVDYAPVKGFMVV
                     KPYGYALWEKMQEFMDKKFKETGHKNCYFPLLIPESLLNKEAEHVEGFAPEVAWVTHG
                     GNKKLEERLCVRPTSETIICTMYAKWLKSYRELPYLYNQWCSVVRWEKSTRPFLRTSE
                     FLWQEGHTLHETAEEAQEETIQQLEVYKALCEELLAMPVVAGQKSESEKFAGGERTYT
                     IEAMMHDGKALQSGTSHFLGQHFTKAFDITFADREGNLANPYHTSWGASTRLIGGLIM
                     THSDNRGLVLPPRVAPIQVVIVPIAAKKGNVMETVDKIYADLKAKGVAVEVDDRDNYT
                     TGWKFNEWEMKGVPVRVEIGPKDIENNQAMVFRRDTLEKDSMPLEGLADAICDLFDVI
                     HNDMFEKARKHREDNTSIVENMDEFRKALEEKPGFIKTMWCGDAECEAKIKEETGATI
                     RCLPFEQENLGHKCVYCGKEADSMVVMAKAY"
     gene            25435..26916
                     /gene="gltX"
                     /locus_tag="ACGI59_001497"
     CDS             25435..26916
                     /gene="gltX"
                     /locus_tag="ACGI59_001497"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFG9739290.1"
                     /translation="MNVRVRFAPSPTGFVHIGSLRTALYNYLFAKKMGGEYILRVEDT
                     DQSRLVEGAIENMLNAMKWAGVNHSEGVILDDSGKVVQKGEYGPYIQSQRLDIYQEYI
                     KQLLDSGKAYYCFCTKERLDEVRDAQRAAGETAKYDGHCKNLSKEEVEANIKAGIPYV
                     IRLRLPENHTIKFTDLVRGDMEFNTNDLDDQVLMKTDGFPTYHFAVVVDDYLMKITHV
                     IRGEEWVSSTPKHVYLYEAFGWEAPVFVHLPNILNKEKKKLSKRQGDVAVEDFKKKGY
                     LPEGLVNYVALVGWSPEDNQELFTMEELEKAFSVERVSKSGGVFDTEKLNWVNQHYIK
                     DGDDAYLTDLAIPFLIEDGFITEEEATNKYDFLKSMISVLKEKLQYVKEVTEHASIFF
                     GDKIEVETEEGREFLRLEHIPTLIDALREKIEKTEVLNAEFVQAMLKEIQKEYKIKGK
                     NLFMGSRIILTGQMHGPDLPKVMEVLGKETCLNRIAYVKNNIL"
     gene            27137..27292
                     /locus_tag="ACGI59_001498"
     CDS             27137..27292
                     /locus_tag="ACGI59_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9739291.1"
                     /translation="MYSNIDDVKKELKELCLEYVTILEKLKDEKMITEETFEKCSSQK
                     KIFLEEQ"
     gene            27437..28834
                     /gene="cysS"
                     /locus_tag="ACGI59_001499"
     CDS             27437..28834
                     /gene="cysS"
                     /locus_tag="ACGI59_001499"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFG9739292.1"
                     /translation="MKVYNTLTRTKEEFVPLEEGKVKMYVCGPTVYNYIHIGNARPFI
                     IFDTLRRYLEYRGYDVTYVQNFTDVDDKIINRSHEEGISPEEVAAKYIKEYFVDCDGL
                     GIKRATVHPQVTDNIQQIIEFIKELEDKGYAYAVNGDVYFDTNKFEGYGKLSGQKQED
                     LEAGARIEVNDQKRHPMDFVLWKAKKEGEPGWDSPWGEGRPGWHIECSVMSKRYLGET
                     IDIHAGGQDLTFPHHENEIAQSEARSGKTFSKYWMHNGYININDEKMSKSKGNFFTVR
                     DISKLYDLEIVRFFMLSAHYRNPVNFSDEMLNQAKAGLERLYNTKEKLEFTLSNLVES
                     PLTEKEVELVKELDDFRQKFIDAMDDDVNTADAVSVIFELAKLINSNVDENSSLEFAK
                     KCLDEFNELTGVLNIVNKKKDTVLDKDIEELIQKRTDAKKNKEFQLADDIRQQLLDMG
                     IVLEDTRQGVKWKRI"
     gene            28837..29232
                     /locus_tag="ACGI59_001500"
     CDS             28837..29232
                     /locus_tag="ACGI59_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III domain-containing protein"
                     /protein_id="HFG9739293.1"
                     /translation="MEKTELVTMSPLVLAYLGDTVYETYIREYLIRQNTQRKVNDLHK
                     LAIKYVKAKAQATIIHEVEIELTEEESKIYKRGRNQKSNTSPKNADIIDYKHATGFEA
                     LVGYLYLNNEIERLQYIINKGIKIIERDM"
     gene            29338..30096
                     /gene="thyX"
                     /locus_tag="ACGI59_001501"
     CDS             29338..30096
                     /gene="thyX"
                     /locus_tag="ACGI59_001501"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HFG9739294.1"
                     /translation="MKVKLISHTPEPEKVIAMAAKLCYSPVGTDEIEKDLTDESIEKF
                     LNMLLSIGHGSILEHASFTFSIEGISRACSHQIVRHRIASFSQQSQRYVKLEQFEYII
                     PPEIEKIEKAKELFIDSMKKDQENYDKLVEILFENHYNDLIKNGKNEKTAKRQAEKKA
                     IEDARYVFPNACETKMVFTINARSLFNFFEHRCCERAQWEIRNLAVEMLREVKKVAPI
                     LFKKTGPSCVNGSCPEGTMTCGDIVQVREKFKAL"
     gene            30109..30846
                     /gene="rlmB"
                     /locus_tag="ACGI59_001502"
     CDS             30109..30846
                     /gene="rlmB"
                     /locus_tag="ACGI59_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFG9739295.1"
                     /translation="MASIEGRNPVIEAIKSDREIDKILIANSAKEGSIKKIIGMAKDK
                     NIIIQYVDKHKLDEVSTSHSHQGVIAYASEYKYYELDELIDLAKNKDEDPFFIILDEI
                     TDPHNLGSIIRTADAVGAHGVIIPKRRSVHITPVVAKASAGAVEYMPVCKVTNIVNTI
                     KRLKEEGLWIAAADMDGETFYKQNLTGPLGVVIGSEGFGISRLVKQNCDFIVKMPMIG
                     NVTSLNASVAGGILLYEIFRQRLDKSK"
     gene            30849..31376
                     /locus_tag="ACGI59_001503"
     CDS             30849..31376
                     /locus_tag="ACGI59_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HFG9739296.1"
                     /translation="MKKNINHYLIIDGYNIINAWDNLKELAKEDLEDSREKLIDDIIE
                     FSEFMGYKTIIVFDAYNVKNSREKVEKRKHITIVYTREHQTADSYIEKFITSLSKYDD
                     VKVATNDYAEQQMILGKGATRMSARELKLELDRSKNKMKEKNIGLRKKIQRNWLEERL
                     DKETLSKLENIRRKR"
     gene            31441..32088
                     /gene="sigH"
                     /locus_tag="ACGI59_001504"
     CDS             31441..32088
                     /gene="sigH"
                     /locus_tag="ACGI59_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigH"
                     /protein_id="HFG9739297.1"
                     /translation="MLVAKEKSYELVDNCQQDEYNIVLRASEGDKIALEYIITKYRNF
                     VKAKAKSYFLIGADKEDIIQEGMIGLYKAVRDFDGSKTNSFKCFAEICITRQIITAIK
                     TATRQKHIPLNSYVSLNKPIYDEESDRTLLDIIATSIVTDPEELIISKEELKNIESKM
                     NELLSDLELEVLELYLNGKSYQFIADKLKRDVKSIDNALQRVKRKLEKHLENRND"
     gene            32162..>32694
                     /locus_tag="ACGI59_001505"
     CDS             32162..>32694
                     /locus_tag="ACGI59_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFG9739298.1"
                     /translation="MAKAKYERTKPHVNIGTIGHVDHGKTTLTAAITKTLYDRYQLGE
                     AVDFANIDKAPEERERGITISTAHVEYETPNRHYAHVDCPGHADYVKNMITGAAQMDG
                     AILVCSATDGPMPQTREHILLSRQVGVPYIVVFLNKCDMVDDEELLELVEMEVRDLLT
                     EYDFPGDDTPIVRGSAL"
BASE COUNT        13290 a         3441 c         6178 g         9785 t
ORIGIN      
        1 ctaatcaaat tttaagataa actctttttt attataaaga taaataacat ataacttcat
       61 attttaggta gattttttct tatatgttta ggagtgcatt taaatttttc cttaaaaatt
      121 ctgtaaaata attttgtatt agtaaatcca tgagcttcta atatactgca taaggtatca
      181 tttgtattaa ttaagtcttt ataaatatga gaaatacgca catcatttaa atactctaag
      241 taagtttgtc ccatatactg tttaaatttt ctacaaaaat attctggttg taagtgtgct
      301 accttagcga ttttgttaat agaaataggc attttgtaat taatctttgt atattctaaa
      361 actggttcaa attttgacaa atcaagtact gtttttttca tatttttatt acctacagat
      421 attttaaagt tagtatataa ttcaaataaa agttcaaata ataagcttgt aaagagcaga
      481 ttagctgcag gaggagaact aagtttaatt tctctcatgt tttctaatat aagtttaact
      541 ttacttgaat tttttttata gttattgtca ttggcattaa ccttaaagtc aaaatagcaa
      601 tttttaaaat cagggatata tttgtttaga aaagacaatg gtacttgcaa taatatagag
      661 gtattttgat aaatacatct agttgagtga ataattccag aattaatgaa catacactca
      721 ttagctttta aattataagt gtaaccctcc atgttaattt gtaaatcacc agaagttata
      781 taaattattt ctaatgagtt atgccaatga tttgctacat aactaccact atcatcaaat
      841 agtttgaaat taacatctaa attatcaata ggttcaatta tttcatgtga aaaattattt
      901 ctgttcataa taatatcctc cttataattt taaattataa atagtaaatt tataatgttc
      961 aatattaaaa agataatata gattaataaa ttagataata aacatactta agaaaacgga
     1021 taaaatcaaa tataataact gtatattaaa ataacttaaa aaaattattt tattataagt
     1081 gcaaaatata cctatttaaa taagataaga ggggaggatt ttatgtgtat ataaaaggtg
     1141 taaatttagg aggttggtta gttttagaaa aatggatgac ctcaagttta tttgaaggca
     1201 cagaagctga agatgaatac tatttaccaa gacagctttc aagagaggca tatgaatcta
     1261 gaattaagac tcatcgtagt gaatacatca cagagagaga ttttgcaatg attaaatcta
     1321 tgggatttaa ttcagttcgt ataccagtac catactttat ttttggagat tgtgaaccat
     1381 tcataggatg tgttaaagaa cttgataaag catttgcttg ggcagataag tatgggctta
     1441 gtatattgat agacttacat acagttccag gaagccaaaa tggatttgat aatggaggaa
     1501 tatcaggtat atgtagctgg tcacaaaatc cagaatatgt agcgtttaca ttaaatgtat
     1561 tagaaagact tgcaaaacgt tatgggatga ggcatgaatt gtatggaatc caaattttaa
     1621 atgagcctat aacagaaaga atgtggaaca taatgaatgt tccaaatcgg tttaaggctg
     1681 tagataagga gatggcaagg gggagtaaac ctaattcatt agagttttta cgtgattttt
     1741 atataaaagc atataaggta atgcgtcctt atatgaggga ggaaaacgtt atagtatttc
     1801 atgatgcatt tgaattaaaa gcatggaaag actttatgag agaggaagaa tttaaaaatg
     1861 ttgttctaga tacacaccaa tatttgatgt tagctgaagc agatggatgt gaacaaagta
     1921 ttgatagcta tctcaaatat attagggaaa attatgctaa agatatttta caaatgcaaa
     1981 aatattttcc tgttatatgt ggggagtgga gcttatttaa ttcatatgct tgtggtatag
     2041 atacaaatgg aggtcaatca cctctaaatg ggattgaatc aaatatagat aaattatcaa
     2101 aagacgataa aagagaatta tataggaaga tagcaaaagc acagcttgat gcatggagaa
     2161 atggaagtgg tcactactat tggaattata agttattact agatacagtt aatgaagaag
     2221 gttggatagg atgggatagt tgggacctag tcaaatgtgt agctcaagaa tggtatccta
     2281 tagaatatta aactaaaatt ggaggaaaag atatgattaa aaaccctata cttccaggtt
     2341 ttaatccaga tccttgtatt tgtagaaagg gagatgatta ttatcttgta gtttcatctt
     2401 ttgaatggtt tccaggtata cctgtttatc actctaaaga cttaaaaaat tgggagttat
     2461 atactcatat tttgacagat gaaacaaaaa tagatttgaa gaagcttcct tcatctaaag
     2521 gaatttgggc accctgtttg acttattgtg aagaagaaga tttattttat atagtttatg
     2581 gaattatgaa ctctatgaat gcaagatatt ttgatgttga taattattta attacatcaa
     2641 aagatataaa gggagagtgg agtgaaccag tatatctaca ctcatcaggt tttgatgcgt
     2701 ctatttttca tgatgatgat ggtaaaaagt ggattgcatc attagactgg gagacaagag
     2761 aaggatatga aaaacctggt gttatatgtt tagtggaata ctgtacaaaa aagaaagaaa
     2821 tagtaggata tccaaagaga atatggtcag ggggaactga tagaggttgt attgaagcac
     2881 cacatataac aaagcgtgga gattactatt atataatgtg tgcagaaggt ggaactggat
     2941 atggtcatag tgtaactatg ggaagggcta aaaatatatg gggaccttat gaaaaagatt
     3001 caatgaatcc aattgtaact tcaattccag gcgattttta tgaaagacat gaccctgacc
     3061 atctaaagcc taaatactat aatccagaat caaaacttca aaaatctggt catggaagct
     3121 atatagaaac aacatcaggt gaagtatatt tagttcatct aacatctaga ccatttgtac
     3181 cagagttaag gtgtacttta ggtagagaaa cagctattca aaaaatgaaa tggacaaagg
     3241 ataattggct tcgaatggaa gatgaaagta atttggcaaa agaatacgta tcagaaagta
     3301 aactagaaga acatttagtt tcatctatac caagttttga tgactttgat tcaaatgaat
     3361 taggtttaca gtactatgct cctaggattt caccattatc atttgcagat gttaagtcta
     3421 gaccaggata tgtaaggatt agaggacaag aatcaagaac atcattaaat aaagtgagca
     3481 tattggcaag aaaattaact tcagtatatg caagaataac cactaagatg gagttttacc
     3541 cagaggtaca tcagcatagt gcgggtttga taatgtatta tgataatatg aattatataa
     3601 atttaagaaa gtattatagt gaaacattgg gtcaaagtgc actatcaata atacatcttg
     3661 aaaatggaga gaaaacggaa tttttaaata ctaggatacc aataaaagat ataccaatat
     3721 atcttcgtct gtatgtacaa ggtcgtaagt catattttga atggagttat gatgaaaaaa
     3781 actaccagag aataggaaag gtatttgata caactaagtt ttcagatgag tactgtaaat
     3841 atggagaatt tacaggtacg tttataggat taacttgtgc agatcgtgta aagcataaac
     3901 actatgctga ttttgatttt tttgaatata tagtagatga atcaaaagat gtcgattaat
     3961 aaaatcatta ctaaaatttt cagagcaaag gtaaatctta tgctctgaaa taagaaacat
     4021 tagaaggcaa atcttacaaa aaggagtatg gtattatgaa tacagaagtt aatgttaaag
     4081 caactacgat tagatatgaa aagatttcat tttttgaaag aatttcttat ggttgtgggg
     4141 atttggggtg caatatcata tactcagcta tgtcagcatt tttgctgttt tattatacta
     4201 attatgcaga tgttagtgca gcagctgtag gtagtattat gcttgtatct aggatactgg
     4261 atgggtttag tgacttaaca atgggaatta tagtggatag aactaaatcc aaatacggta
     4321 aggctaggcc ttggattttg agaatggcga ttccttttgc tatagcagca gtattattat
     4381 tttctgttcc atcaaatctt ggtatcactt ctaaattaat ttatattttt attacatata
     4441 acttagtttc tactgttatt tatacagcaa tcaatgtacc ctatgcaact ctaaattcac
     4501 ttataactca aaaccagtat gaaagaggag tattaagtat atttagaatg atattagcca
     4561 cttgtggtac cttactaata aatggactta cactacctct agttgaatac tttggaaata
     4621 atttatcagc atggactaaa acattctttg tatttggaat agcatctata atggtatttt
     4681 ttataacatt tactggaacc aaggaaagag taaaagcagt taagcaagat aggaatgaag
     4741 taataccatt taaaataggt attaaatctt tatttagaaa taagtactgg atacaaataa
     4801 cattatgttt agtttgcata tttattgtat ttgcgataaa tggtggttca tccgtatatt
     4861 atgcaaagtt tattttggga gatgaaaaat tatttggtcc tataaatatg gtttcaaata
     4921 tatcccaaat tatagctatg tttatggttg ctccttttat aaaaaaattt ggaaaaagaa
     4981 atgtattaat agtaggttca ataatattaa tatcgtcaaa tgttatgttt ataatagttg
     5041 gacaaaatca tataggagta atatttgcaa gtgctataaa gggaatagga agtgctggaa
     5101 tagcatcaac aatgtttgct attgtttcag atactattga atatggagaa tggaagactg
     5161 gatatagaac tgaggggctt ataaatagtg catctagttt tgggtttaaa gtgggaaatg
     5221 gattaggttc agcaatatta ggtgcagtat tatcaattgg tggttatgta ggaacatcag
     5281 ctactcaaag tgatttagct atagtatcaa taaaagtatg ttttatatac ctacctatat
     5341 ttataacaat attacaaatt atcataatgt ctttttataa gcttgataaa gaatatagta
     5401 ctatacttaa tgaattaaat actaaataat gaaaaagttt cttataaagt cttaagttta
     5461 attaaggtct attgtaaatg ttccctataa aaactattat atatttatga ttattgaaga
     5521 atttttagag aagcgaataa taaaaatata agaagacttt aaatgatgaa atggttggtt
     5581 ttttagaagt gatttaataa gttattgtta tttaaagcat tataagtgga caattaattt
     5641 ttaatttatg gagatgatat ttaacaatta gaagtaatat ataatatgca ctaagaagca
     5701 atttttcaac tatttatgaa ggtggtggta caagaaaagt atcacctttt ttttgttata
     5761 caaaaaaaca gttgactttt tatatgaacc ccttctataa tgttactgaa attaagtaaa
     5821 atgattgaac aaatgtaaag ataattgaaa agcttgattt tggaggaaag caaatgaaaa
     5881 aaattggaga cttaagatta atgatgaagt gttgttcact ttattacgaa gataatttga
     5941 atcaacagga aatagcaaat caattgggaa tatcaagacc aacaatatca agaatattaa
     6001 aagaagcttt tgaacaagga atagtaaaaa tacagatagt tgatgttttg aaaaatgatt
     6061 accaaaaaat agagagaagt ttggagagaa aatataaatt aaaagaagta atagtagtag
     6121 atgataaaca ggatgcacta acacaaaaac aagaactagc aagagctgta tctgaatatt
     6181 taactagagt agtaaaggaa aatgatataa taggtgtttc aataggaaca acactaaaag
     6241 aaataccaag atatgtggaa aaaagtaact gtaaaaatgt tacatttata cctctattag
     6301 gagggattgg agacaatgaa atagatattc atgcaaatca aatagccgta agtttagcga
     6361 gggcttttgg aggtgatttt aaattattac atgctccagc tgttatgtct gacttgtcta
     6421 caaaagagaa actttgcaaa gacgaaaaaa ttaaagaagt actagattta atagataaaa
     6481 caactatagc aattgtgggt ataggaaatc ctatgagttt aaactcaaca ataatggctt
     6541 ctggatacat gaatgaagca gatatagaag atttaaaaaa atacaactca ataggtgcaa
     6601 tttgtttaca agcctttgat aaagaaggaa aaacttctat acttgaattt aatcaaagag
     6661 tattaggagt gaaactggaa gatttaaaaa agataaaaag aacaataggt gtcgcttcag
     6721 gagatgaaaa aatagaggca ataaaggcat cattaaaagc gaaatttata aatagtttag
     6781 caattaatca cagtttagca cttaaattat tagaagattg tgactaatag aaagatagat
     6841 aaaagttaac aggagggaat gtatatgtca atatcaaaag aaacattgct tgaaatgtat
     6901 aagagaatga atcaggctag aaaatttgaa gagaaagtta gttggttttt tgcaagaggt
     6961 atggttcatg gaacaactca cttatctgta ggtcaagaag cgtcaagtgt ggcggctgta
     7021 atggctttag aaaaaggaga tttagtatca ttaactcata gaggacatag tcaatttata
     7081 ggaatgggaa tagacctaaa taaaatgatg gctgaactta tgggtaaaga aactggattt
     7141 tgtaagggaa aaggaggctc aatgcatata gcagatattg agtcaggtaa cttaggagca
     7201 aatggagttg taggtggagg tcttacaata gctccaggag ctgcacttac acaacagtat
     7261 aaaaaaacag gcaaaatagt tttatgtagt tttggtgatg gagcatcaaa tgaaggtact
     7321 tttcatgaag gaataaattt atcttcaata tggaaattgc caataatatt ttactgtgag
     7381 aataatttat acggtatgtc aacaagcata aaacgtcata tgaatataga aagtattgcc
     7441 actagagcag cttcttatgg aatagaagga atatcaatag atggatataa cccaatagaa
     7501 gtatacgaaa cagtacaaaa agctgctgag aagtgtagaa gaggagaagg acctgtatta
     7561 attgaaagta gaacttatag atggttaggt cattcaaaat cagatgcaaa tgtatataga
     7621 acaaaagaag aaatagaatc atggaaagca aaagacccta ttgaattttt aaaaaattat
     7681 cttatagaaa ataatctatc taatgaagat gaattagata agattcaaga atttgcaaag
     7741 caatcaatag aagatgcagt agagtttgct caaaatagcc caaatccaaa aatagagtct
     7801 ttattagagg atgtatatgc agactaagaa tggtaaagga ggaaaataaa aatgagtaca
     7861 agggaattaa catatgcaca agcaataaag gaagcaatgt cagaagaaat gagaagagat
     7921 gaaaatgtaa tatttatggg agaagatata ggaatatatg gaggagcatt tggtgtatca
     7981 gttggtatga tagatgaatt tggaccagaa agagtaaggg atactcctat atcagaagca
     8041 gctatagcag gagcggcagc aggtgcagca gcaactggct taagacctat tatggaagta
     8101 atgtttatgg actttgtaac aatttctatg gatgcaattg taaatcaagc ggcaaaaatg
     8161 agatatatgt ttggaggaaa agcacaagtt ccaatggttg ttagatgccc aggaggttct
     8221 ggaactggtt cagcagagca acattcacaa agtttagaag cttggttttg tcatgtacca
     8281 ggagtaaaag tagttgcacc ttctacacca gcagatgcta agggattgtt gaaagcagct
     8341 ataagagata ataatccagt catatttgtt gagaataaat tgttatacag aaaaaaaggt
     8401 tttgtgccag aagatgacta tgtaattgaa attggaaagg cagatattaa aagagagggt
     8461 actgatgtta cagtaattac ttatggaaga atgttacaaa gtgttgaaga agcagcagag
     8521 actctatcaa aagaaaatat aaatgttgaa attatagatt taagaacttt atatccatta
     8581 gataaagaga ctatagtaaa gagtgtatgt aaaactggga gagttttaat ttgccatgaa
     8641 gcagctaaga ctggtgggct aggtggagaa atatcagcac tgataacaga aagtgaatca
     8701 tttgattatt tagatgctcc agtaaaaaga atttgtggaa aagatgtgcc aataccatat
     8761 aatccagagt tagaaaaagc agttgtccca agagttgatg aaatagaaga agcaataaaa
     8821 tctttaattg ttagataagg agggatagat atggtggcaa ataaaataaa agccacacca
     8881 gcagctagaa gtcaggcaag aaaagacaat ataaaattag ataggctgat tggaagtgga
     8941 gaaaatggaa ggattcattt agtagatgtg ttaaattatt taaaagataa taaagcaaat
     9001 actactcctt tagctagaag aatagcagaa gatttaaata tagatttaga aactattgta
     9061 ggtactggat ataatggaaa gataagaaag tgtgatgtgg aaaaattaac tgcaaaagaa
     9121 actattgtat cgactaatac atctaaatct agtgagaaaa aagagctgaa aattgaaaat
     9181 gaaaattcaa gcatgtttaa cacagtagaa ggaatatttg aaaagccaaa tccaatgaga
     9241 gctactgtag caaaacgaat gtcagaaagt tattttagtg ctcctgtatt tacattcaat
     9301 atagaagtag atgctacaga attgaaggta cttagagcaa agttaatcga cacagtaaaa
     9361 gaatcaactg gagtaaaatt aactatgaca gatttaatag taatggcagt atctaaaata
     9421 cttccaaatc atcaagcctt aaattcagca tggacagatg agggtatatt tagatataaa
     9481 gatgtaaata tagctattgc agttggttta gatgaaggct tatatgttcc ggtagtaaaa
     9541 aatgcaaaca aaaagtcttt aaaagaaata gcaaaagaaa gcaaagaact tgcggaaaaa
     9601 gtaaagacag ggaaacttat gccagcagac caagaaggta atacatttac aataagtaat
     9661 gttggaatgt atggtataac tacatttaca cctataataa atatgccttc aagtgcaatc
     9721 ttaggggtag gtgctacaca agataagttt gttccagtaa atggagaggc taaaataaaa
     9781 cctataatga acttatcatt aacatcagac catagagtaa tagatggaac agtagctgca
     9841 aaattcttaa aagatttaaa agaattgtta gaaaatcctt tatcaatgct tgtataggtt
     9901 ttgatatttt gttactgata taaatagttt gaatagatta tattattata attgtttcag
     9961 taaatttaaa gcttttatat attaagaatt atttttaaca gtaactatat gtgaatatga
    10021 tttgcttaat taaaatataa gaaaataaat aggaggagag cctatgtcag tagaagtaat
    10081 aatgccaaaa gcaggagtag ccatggaaga aggtactata gtatcttggc taaagcaaga
    10141 aggcgaagaa gtaaagattg gagaacctat actagaaata acaacagata aagttaatat
    10201 ggagatagag tcagaaggag aaggtacact tgctgtaatc atacataaag aggaaggtga
    10261 agtattacca gtatttacag taataggtgt aatagcagaa aaaggggaaa atcaagagga
    10321 agtaaaagct aaatatttat ctggaaatgt ttcaaaagaa gatatagttg aagagaatca
    10381 aaatatagaa tttaaagaag aaaaaataaa caaaaaagag tgtaatcatg attatgatgt
    10441 agtggtaata ggaggaggac ctgggggata tttatcagca ttaaaagcag ctcttttagg
    10501 tggaagagtt gctttagttg aagaaaatat actaggaggt acttgcttaa atagaggttg
    10561 tatccctaca aagacttata taaaaacagc agaaatatta gaagaaattg accaattatc
    10621 aaagagaggt gtaaaagtaa ctgtagataa agaacaggac attaagaagg ccataaaata
    10681 taaaaataga gtagttaaaa aacttacagc tggtgttggc ggattattaa aaagtagaga
    10741 tgtagatgtg tttaatctta aagctagtgt taaagaagag cataaagtaa ttttatcaga
    10801 tggaaaagtt ttagatacag aaaatataat aattgctaca ggttccaaag taaggatatt
    10861 acctataaaa ggaatagaat caaatttaat aataactagt acagaagcat tagatttgga
    10921 aacagtgcca gaagaattag taataattgg tggtggagtg attggttgtg aatttgcaga
    10981 gatattcaat tcaagaggtt ctaaagtaac tatagtagaa atggaagata gagtaatccc
    11041 aagaatggat aaagagttaa gcgaatcttt aaaatattct cttagtaaaa aaggtataaa
    11101 tgtattaact aaaaagaaag tttctgaatt taaagaagaa ggaaataaca tattagtgtg
    11161 cattgaaggt gaagagccaa taaaagcaga tttatgtcta tatgctatag gaagagaagc
    11221 taatctttct ggcatagagg acttagatat taaaatagat aaaggttcaa tagttgtaaa
    11281 tagtaaaatg gaaacaagta taccaagcat atatgcagta ggtgatgtta ctggaggtgt
    11341 aatgttagct catgctgctt ttaaaatggg agaggtagca gcttcaaatg ctctaggtgt
    11401 gaataaagaa gttgacttag gtgcattacc tagttgtgtt tatacaattc cagaagtagc
    11461 atcagttggt attacagaag aggatgctag aaaaaaatat aatgtaaaag ttggtaagtt
    11521 taattttgct ggaaatggaa gagctttagc ttctggtcaa gaacaaggat atgtgaaggt
    11581 agtagcagat gctaaatatg gagaaatttt aggtatacat atgtttggat gtggtgtagc
    11641 agaacttata aatcatgcag catcatttaa agcattagag atacctactg atgaagcaag
    11701 tgagttgata tttggtcatc catgtacttc agaagcttta atggaagctt tggcagatgt
    11761 aaatggagaa tgtttacact tacctaaaaa ataaatctaa acataaatta ttaatttaag
    11821 tctattatta atattaattt atatcataaa attttaaaac acttacttag ccatacccct
    11881 tattatgcaa ataatttatt tgcataataa ggggtatttt ttatggaaaa atatagaata
    11941 tagaatcctt gtagtttttg ttgtacaaaa aagatagtaa tataaaataa cattatcatt
    12001 ttagaatgat ttcaattgta attaggaaaa gtgatttttt tgtttttaaa aaaagtatta
    12061 tttgtaaaat ataaaaaaaa atttcttaat atttaaataa aagaatagct taatttaaat
    12121 gttattactt tataaaaaaa taataagtac aaaaaaaaca atatatggca tataagttgc
    12181 tatataaata ataaggaggg aacaattata aatgaggcaa agaataaaaa acttaattga
    12241 aaatgaagat aagaaaaatc ctttaacaga tgaagcgatt tcaagtaggt taaatattcg
    12301 aagagaagat gttacctttt tgagaaatga gttaaaaata gaagattcaa gacaaagaag
    12361 aaaagttgtt ttaattaaag ctataaaaga tattttaaaa gaagaaaaaa ctataaataa
    12421 aaatgatata acaagaagat taaacagcat tgggtttaca gtatcaagat ttacagtcat
    12481 acaatactta aaagagattc aagattctgg tgacctaaaa cttgataaag agagtgatga
    12541 taaaaaagaa gaaataatca ataatagaga aaataaagat atagcatttg ataaactcat
    12601 aggtagtagt ggtagcttaa gtacaattat aaaactagcc aaagcagcaa ttctttatcc
    12661 acctcatggt cttcatacta ttattatggg gccaactggt gttggaaaga gtgagttagc
    12721 agagtgtatg tataaatttg cagtagaatc atccagattc cctaagaatg caccatttat
    12781 agtttttaat gcagcagatt attcagagaa tccacagctt ttgctatcac agttatttgg
    12841 acacgttaaa ggtgctttta caggagctga tgagaacaaa gaaggattag tatcaaaggc
    12901 agatggagga atattgttta tagatgaaat acatagatta cctcatgaag gtcaagaaat
    12961 actatttcaa ctgattgata aaggtatggt cagaaagtta ggagaaacaa gactaacaca
    13021 taaaatagat gtaatgatta tttcagcaac ttctgagcca atagattctc atttacttaa
    13081 tacatttaaa agacgtatac ctgtaacaat tgaaatacca gaattagttg caagaccatt
    13141 aaatgagagg tttgacatta taaataattt cttcttaata gaagctcaaa gaatgggagc
    13201 aaacatacat ataaagtcag atgtattaaa agctcttatg ctttacgatt gtataggtaa
    13261 tgtaggtcaa cttagaagtg atattcaagt agcttgtgct aggggactat taaaccaact
    13321 tacaaacaaa ctaaaagaag taaatataag catatcagat gtacctggat atgtaaaaca
    13381 aggtcttata aaaatcagaa attgtagagg aaaaatagaa aattatgtag atggggattt
    13441 aattatagac tcaaatcttc aaggtgagat agaaaagaaa aatgaagata tatatacttt
    13501 tccagatgaa atatacaaac ttactgaaag aagacatatt gatttattgg aacaaggttt
    13561 agattttgat gttataaata gaatcattgg aggagaatta gaaagttgca ttcaaaaata
    13621 cataaaacaa gttaaaaaag tttctattag taacaatctt ccagatataa gtccaattgt
    13681 tggaaaatat atcgtagagc taacttatga agtaatcaaa atagcatcac aatacctaag
    13741 ttcactagaa ccgaatctac atttatgttt agcagttcac ctaaaagcag cttttgagag
    13801 attaaaagaa ggaaaagtaa tatcaaatgc tcatttgaaa gaaataatag gaaattatcc
    13861 actagaatat tctgtagcag aaaaggtagc tcaatattta aaggaaagat ataacataaa
    13921 cttaccaaaa gaagaaatag gtataatagc aatatatttt aaaatgagta gtagaagaga
    13981 tatgtctgaa caaaaaaaga taggtgtact tcttatggct cacggaagag tagcatcttc
    14041 tgtatgtgag gttacaaata aactattagg aattaatcac gcaaggtatt tagatatgcc
    14101 atttgagaag aaaccggaag aaatgattga agaagctact acaataataa aagatattga
    14161 tgaaggtaga ggtgtgatta tattagtaga tatgggttca ctttcatatt ttggagatat
    14221 tatttctgga aagactggaa ttaatataag gacaatatct agatttgata cattgctttc
    14281 tatagaggtt atacgaagag cagttttacc agatttaaca atagacgaga tagttgatga
    14341 agttaaaggt atagagccac aagtaaaaga aaaggttagt aaatgtaata atacaaaagt
    14401 agattttaga aaacctataa ttttaacatt atgtattact ggattagggg gagcttgtaa
    14461 gataaaaaaa atcctagagg aaaatattaa aagtataact gaaaaggttg atatagaacc
    14521 tataggtatg attgaatatc caagtcttga tttagagata aaacaaataa gcaatgataa
    14581 aaatgtaatc gccatagttg gaacaattga ccctcagata gcaaatatcc ctttttttag
    14641 tctagtagac ataaatgata aagcaaagtt aaatcaaata aataacctaa ttgatacaaa
    14701 tcaaattggt gaaacaaata aaaaatatga attatcagat ttaatacata aagaagatat
    14761 atttgtggat ttacaggtta aatcaaaaga agaagttcta aaatatatga gtaaaaaact
    14821 tagagagaaa ggttatgtga caaaaaattt ctataaaaaa gtagtggaaa gagataactt
    14881 atttccaact gaattatcag atgaaatagc aatacctcac acagatggaa tagatgtaat
    14941 taggccagct ataagtatag ccatcttaaa gaagcctatt gtgtggagta aaagtaaagt
    15001 aaaaataata cttttattag ctgtagacca aaagtgtttt aatccattaa gcactctact
    15061 ctcatttata gaaacagatg aatttaagaa gataaaaaac attaaagata gagattttat
    15121 tagggagatg atattagatg gagtcaatga aaataattag tagcaattta atttttaaaa
    15181 atattgaagt gtctaacaat gaagatgcat taaagttttt gggacaacgt ttgtttgacg
    15241 aacagtatgt aaaagaaagc tacatacaag ctgttgtagc aagagaaaaa aagtatgcta
    15301 cagggttacc aacagaaata tacggagtgg caataccaca tacagatata gtacatgtaa
    15361 atgagccagg aatagcaatt ggaatactga ataaacctgt aaaatttata atgatgggta
    15421 cagacgatac tgaaattgat gtaaaagttg tatttatgtt agcagtgaaa gagccacaag
    15481 aacaattgca actattagaa aaacttatga ctatatttca agataagaat atactaaata
    15541 atattgttga cttaagtgaa gaaagtgtga gtgatttatt aaactctaaa ttgaaaaatt
    15601 aaacatgtaa agtatattaa aaatgtaaaa ttaaacatgc aaagtatgtt aaaaaatata
    15661 aaaagattat taaagatttc taactataga tatctttaaa ataaaataat ttcaatgggg
    15721 gtgactaatg tggctaaaaa gatattagtt gcatgtggaa caggagtatg tacttcaaca
    15781 attgctataa acaaattaaa gaaagcactt caagacattg gtaaattgga tatggttaat
    15841 attacacaat gtaaagtagt agaagttgca tcaaaagcac ctgattatga tttaataatt
    15901 tgtactacac aagtatctag tagtataaaa acaccagtga taaatggatt acctttttta
    15961 actggtgtcg gaatggataa attaatcaat gatgtattag aagagttaga attataatta
    16021 aaaaaattaa ggaacatggt ctctaaaatc ataagtttta gagacttaaa attaaataat
    16081 ggagggtctt tatatggaga tattaaattt cattgttggt ctaggtgcac aagtaatgat
    16141 gccaataata atatgtattt ttggtttgat attaggtaca aaactaggaa aatctttaag
    16201 agctggttta actgtgggag ttgggtttat aggtcttaac actattatag ctttgctaac
    16261 agataattta ggtccagcta cacaacaaat ggttaagaat ttagggctaa gtttatcaat
    16321 aatagatgta ggatggccag cagcatcagc tatagctttt gcatcaacag taggggcttt
    16381 aattattcca ataggtcttg tggtaaatat agttatgcta attaccaata ctactcaaac
    16441 tgttgatgta gacatatggg attactggca ttttgctttt acaggagctt tagtggcagg
    16501 ggctactcaa agtgtaatgt ggggagtatt tgcagcagta gcaaatatgg taattgtatt
    16561 ggtaatggca gatttaactg ctccaggtat agaggaatat ttaggtatgc caggtatatc
    16621 tcttcctcat ggatttacac aggcatttgt tccaattgct atagtagtaa ataaattatt
    16681 agacttaata ccaggcataa ataagattga gataaatgct gatacattgc agaaaaaatt
    16741 tggattattt ggagaacctt taataatggg gtctgtaata ggtgtaataa ttggtattgc
    16801 tgcgaaatat gatataaaag gaatattaca attaggtgta actatgggag ctgtattaat
    16861 attaattcca aagatggcag cattattaat ggaaggatta ttaccagtat cagaagctgc
    16921 acaggaattt attgagaaga gatttaaaaa cagaggcaag atttatatag gcttagattc
    16981 agcagtagga ataggtcatc ctgttacatt atcagttgca ctagtgttgg ttcctttgac
    17041 tatacttata gcagctatat tgccaggaaa taaagtttta ccatttgcag atttagcagt
    17101 aattccattt gcacttgtat taatcgtacc aattacaaag ggaaatgtgt ttagaacatt
    17161 aataatagga ataattataa ttacttctgg attattaatc gcaactaact tagctccatt
    17221 atttacccaa atggctttaa atgctagttt taaaatgcct gaaggagcaa ctatgatttc
    17281 aagtatttgt gatggtgcga atccattaag ttgggtattt gtaaaagtta tgaactataa
    17341 agttattgga ggagtagttt ttggagttat agctttagga atggctattt acaataggaa
    17401 tagaataata aatgaaaata aaaagctatc tatagaagaa taaggttaaa acaattttat
    17461 taaggagtgt ataggttatg aaaaaatata aatatgattc taaaagatac ttactcagtg
    17521 ttacaatacc aggatttttt atgattttaa tattgattta tgcactgttt aataactata
    17581 caaattttaa tttgaatata tatagtttgt taataattgt ctgtacttat ggtatattca
    17641 atacttttat atcatcaagt aatcctaaaa aaattattat tgatagcaaa tgtatacatt
    17701 ttacctcatt tatgactaca cataagtatg aaattaaaga tatagaaaag ataagaataa
    17761 gagaatttta taataaaaaa atttatctaa aaattaatga tggaaaactt ttcaaaggta
    17821 ggtattggat aagaacaaat atgtttaata atagtatagc attatatagc tatttcattg
    17881 agttggaaga gtgcttatat ccagattcac taaaatttag aaataaaaaa tttgagaata
    17941 agaacatata aaaataaaaa attgaattga agcctagctt aatctttttt gataagatat
    18001 aagtttgaac tttatactaa atttttagaa ttttcaatca agacaataac ataatagcta
    18061 agcaattaga acacaactaa gatgtataag gcatgtatat gatgaatggg aggatttaat
    18121 taatgttatt agagaaatta agaaaagaag tattacaagc atctttagac cttttaaact
    18181 ataatttagt gacattaaca ggaggaaatg taagtgggag agatgaacaa actggctata
    18241 tagcaatcac cccaagtgga atggattata gaaatcttac accaagtgat attgttatag
    18301 tagatgtaga tggaaatatt atagatggaa aatggaaagc atcagtggat ttatcagacc
    18361 acttgtatat atataagcat agagaagaca taaatagtat aattcataca cattctacat
    18421 actcaagttg ttttgctatt ttaaatgaac ctatagaatg tgcaagtaca actcttgcaa
    18481 atgaagtagg gggttcagta ccagttgcaa aattttcgcc accaacatct aaaaagatgg
    18541 gaaaatgtgt tattgaagca attggtgaca aaagagcatg tcttttagca aatcatggcg
    18601 taattgcagt tggtccatct gtaggacatg ctcttacagc cgctgtaatg ttagaagatt
    18661 cagcaaaagt atattactta gcaaaatcaa ttggcacacc agtgctactg cctgatgaag
    18721 aaattcaaag agcgagagat gtatttttta atgtctatgg tcaggataaa taaattagat
    18781 aaaattttaa aagtaatatg aaaatataga tattcaatga tattaaggag atttataatg
    18841 atataataag tttaatagtt aagaagtaaa atatgaaatg taatagtata taaattcatt
    18901 taagactaaa aagaataaga taaaataaaa aggagtctgt atttctatgt attacttatt
    18961 aaacaaaagc acaaatcctt actttaattt ggcattagaa gaatatctat ttttaaatga
    19021 taaatataat gatgatatta taatcatatg gagaaatgaa gaatccattt ttataggtaa
    19081 aaatcaaaat ccatatcagg aagtatatca tgatgttatt gaaaagggag aaattcctat
    19141 tttaagaaga atatctggag gaggaactgt ttatcatgac ttaggtaata tcaatatgtc
    19201 attcatacaa aaagataggc aacttcatga gatagatttt ttggagcata ctaagtttat
    19261 gcaaaagatg ctttcaacct taggactaga tgtatctata actgaaagaa aagatttatt
    19321 tttaaatggg aaaaaaattt caggctctgc acagtctata aaaagaaaaa attctcttta
    19381 tcatggaact cttctttacg attcagattt aaataaatta actaaatatt taaattcaaa
    19441 taaagcaact gaatcaaatg caactaaatc tgtttcaagt aaagttacaa atattaagtt
    19501 tttactcgaa aaagatataa attacttttt agattattgt gttgaatatt taaaatctaa
    19561 tataaagaat ataaaagaac ttgaattgga tgaagaagat attaaaagtg tatacaatat
    19621 aggagaagat aaatataaaa agatagagtg gacatatggt aaaacgccga aatttcaagt
    19681 agtgctacca tttaataatg aaagtaaaat aaatatacat gttaacagat ggaaagtatc
    19741 aaaatttttt atttcttatg atgataatgt aattaatatg gacaagttgt tagatttgaa
    19801 tttctttaaa gaagaaataa aagagagtat tttagaaaat tatccacatt acatagattt
    19861 aatcaattta atattttaat ataaaacctc taatttgata aaattgtctt atatcaaact
    19921 agaggttttt aatattcttg catattaaca accattacaa aattgtaaaa ttaaaaacaa
    19981 cactattgaa aaatcaatat atattgtaat aaatatatgt gtttttcatt agcaaataga
    20041 cattaactat ttatacaact gaaaatttaa tatataatta atcagatggt taaaataaaa
    20101 atattaaaag ttttaataaa tgatggttaa aaataaaata cgaggtgata ccatgtatag
    20161 tgttataatt gttgcagcgg gaagcggaag gagaatgaac ttagatataa acaaacagtt
    20221 cataaaatta agagaaaaag aaattattgc tcatacaatt caagtttttt atgaaaatat
    20281 aaatatagat gaaatagtgg tatgtataaa aaaagaagaa gaagattttt ttaaagaaaa
    20341 tataataaat aagtataact ttaaaaatat aaaaatagca tatggcggaa aagaaagaca
    20401 ggactcaata tataatggtt taaaaaaatt agataaaaat tgtgatatag tacttatcca
    20461 tgatggagca agaccatttg tcgaccatag aataatcaat gaatctataa aggtagctaa
    20521 agaaaaaaag gctgtggttg taggtgttcc agtaagtgat actataaaga tagtgtcaga
    20581 tggaacagta caagaaactc cagaaagaaa tttgctgtgg gcagcacaaa cccctcaaac
    20641 ctttgaatat aatctcataa tagatgctta tgaacaagca tataagaaca attattatgg
    20701 tactgatgat tcaatgttgg tagaaaatat aggtcaaagt gtcactatgg taatgggttc
    20761 ttatgaaaac ataaaaataa ctagtccaga agatttaaat atagcagaac aaatattaaa
    20821 tatggaaaaa agagatgaag ttaacagtag aaggagaatt atatgagaat aggattagga
    20881 tatgatgtac acaaattaac agaggataga aaacttataa tcggtggtgt agagatacca
    20941 cacgataaag gtttgttggg tcattctgat gctgatgtat tgatacatgc aataatggat
    21001 tctattttag gtgctctagc attaggagat atagggaagc atttcccaga tacagatgaa
    21061 gaatataagg gagcagatag tatgaaatta ttagaacatg tgtataatct aataacaagt
    21121 aaaggatata aaattggtaa tatagacagt actataattg ctcaaagccc taagatggct
    21181 ccatatattg agagtatgag aagtaatata tcaaaagttt taaacacaga cattgataat
    21241 ataaacataa aggcaacaac agaagaaggt cttggtttta caggagcaaa acagggaata
    21301 gcttcacaaa gtatttgttt attattgttg acaagtcaga ataattaata tattattagt
    21361 aataagttta atggatatca agtaataggc ctatatagaa tatctataat gtatgaattt
    21421 aagtgaaatt aatataaata taaaattagc tatgataaag aaatagtaaa atgatgaaat
    21481 cttcacagag agaaaacggt ggtgagagtt ttcaagagga agtctttgaa cctgtctttt
    21541 agcttaaagg tataaaaatc tttacggtag gaaccgttat attcccaaaa gagagctatt
    21601 catatagcta actagagtgg taccgcggaa attaaacctt tcgtctctat acttgtatag
    21661 agatgagagg ttttttatat tttcaggaag ttaatattca ggaggaatct attaatgaaa
    21721 atgtcaaaaa tgtttatgcc gacattaaaa gaaatcccag cagatgctga gattacaagc
    21781 catcaattaa tggttaggtc aggcatgata aaaaaaatga cttcaggtgt ttataaccaa
    21841 ttaccaatgg ggttaagagt gttcaaaaaa attgaacaaa taattagaga agaattaaat
    21901 aaaaaagatt gtcaagaaat actttgtgca gccttacttc cttctgaatt atggaaagag
    21961 tctggaaggt ggactgctat gggtgaagaa atgtttagac taaaagacag aacagaaaga
    22021 gagtactgtc ttgggccaac acatgaagaa gcattcactg atataataag acaagaaatt
    22081 acttcatata aacaattgcc actgaacttg taccaaatac aagtaaaata tagagacgag
    22141 agaagaccaa gatttggtgt tatgagaact aagactttta caatgaagga tgcctatagt
    22201 tttgatgtag atgataaagg tttagataag tcatatcaag atatgtttga tgcatatgta
    22261 agtatattta atagatgtgg attggagaat agccctgtac aggcagattc aggagctata
    22321 ggaggttcta catcagccga atttatggtt aagtcagaag ttggtgaaga tgaagttgta
    22381 ttttgtagtg gatgtgatta tgcagccaat gtagaaagag cagaatcttg taatctagca
    22441 tcacaaaaag aagaaatgaa agaattagaa gaggttcata caccaggagc agctacaata
    22501 aaagagttag aagaattttt aaaaacttct ccagataagt ttgctaaaac tttagtatat
    22561 gaggcagatg gaaaaacagt tgtagtcgtt gtaagaggag atagagaagt taatgaaatc
    22621 aaagtatcta atgccatagg ttctgttata gaatttgctc ttgctacaga tgatgtagtg
    22681 agaaaagtaa ctaatgcaga agtaggattt gcaggtccaa taggtataaa tgctgactat
    22741 gtatttatag ataaagaaat tgttgaacaa agaaacatag ttgttggggc taataaaact
    22801 gagtaccata taaaaaatgc taactatgga agagattttg aaggaatagt tggagacttt
    22861 agaaatgttc aagaaggcga caagtgtata gtatgtggta agccacttga aatagctaga
    22921 ggtgttgaag taggacatat atttaaacta ggaactaagt actcagaatc tatgaatgca
    22981 aacttcatag acaaagatgg aaaatcaaag cctatagtaa tgggatgtta tggaataggt
    23041 gttgaaagaa cagcagcagc tataattgaa caacataatg atgagaaagg tataatatgg
    23101 cctttgtcag tagctccata ccatgttgtg atagttccag caaatatgaa aaatgaagaa
    23161 caaatatcaa tagctgaaaa tatatataat gatttgcaag ctatgggtgt agaagtttta
    23221 ttagatgaca gagatgaaag aataggtgtc aaattcaatg attcagaatt aataggaata
    23281 cctatgagga ttacagtagg taaaaatatt aatgaaggta aagtagaatt taaacttaga
    23341 cataaagaag ataaagagat aattgacata gaagaaataa atgaaaaagt aaaggcagag
    23401 tttatcagga ataatgttag gttaggataa taacttaaat aagatattgt atagataatc
    23461 aaagttgtac tttatagaca ccattttatc tatatataag gatataaaat ggtgtttttt
    23521 tataaaacaa ataaatgagg aggtaattaa aatggcaaaa aatgaaaaac aatttgttga
    23581 agaaataaca aagatggaag atgattttcc acaatggtac acagatgtaa taactaagac
    23641 tgacttagtt gattatgccc cagtaaaagg ttttatggtt gtaaagccat atggatatgc
    23701 attatgggaa aagatgcaag aatttatgga taaaaaattt aaagaaactg gacataaaaa
    23761 ctgttatttt ccattattaa taccagagag tttattaaac aaagaagcag aacacgttga
    23821 aggttttgct ccagaagttg catgggtaac acatggagga aataaaaaat tagaagaaag
    23881 attatgtgta agacctactt cagagactat aatatgtaca atgtatgcga aatggttaaa
    23941 atcatataga gaattaccat atctttataa tcaatggtgc tcagttgtta gatgggaaaa
    24001 atcaactaga cctttcttaa gaacatctga gtttttatgg caagaaggtc atacgttaca
    24061 tgaaacagca gaagaagcac aagaagaaac aatacaacaa ttagaagtat ataaagcgct
    24121 atgtgaagaa ttacttgcaa tgccagttgt agctggtcaa aaaagtgaaa gtgagaaatt
    24181 tgcaggtggt gaaagaacat atactataga agctatgatg catgatggta aagcattaca
    24241 atcaggtaca agtcacttcc taggtcaaca ctttactaag gcttttgata ttacttttgc
    24301 agatagggaa ggaaatttag ctaatccata ccatacatct tggggagcat ctacaagact
    24361 tataggtgga cttataatga ctcatagtga taacagaggt ttagtattac caccaagagt
    24421 agctccaatt caagttgtta tagttcctat agctgctaaa aaaggaaatg taatggagac
    24481 tgttgataaa atatatgctg atttaaaagc taagggagta gctgtagaag ttgatgatag
    24541 agataactat acaactggat ggaaatttaa tgagtgggaa atgaaaggtg ttccagtaag
    24601 agttgaaatt ggaccaaaag atatagaaaa taatcaagct atggtattta gaagagatac
    24661 tttagaaaag gattctatgc ctttagaagg attagctgat gctatatgtg atttatttga
    24721 tgttatacac aatgatatgt ttgaaaaagc aagaaaacat agagaagaca atacatctat
    24781 agttgaaaat atggatgagt ttagaaaagc tttagaagaa aagccaggat tcataaagac
    24841 tatgtggtgt ggagatgcag aatgtgaagc taagattaag gaagaaacag gagcaactat
    24901 aagatgctta ccttttgaac aagaaaactt aggtcacaag tgtgtgtact gtggaaaaga
    24961 agcagatagt atggtagtaa tggcaaaagc ttactaaaat acatttaaat aaaaactggg
    25021 gtagaaagta tttaatactt tatcccagtt ttttgttttt tatgagtaat aaaatgtatt
    25081 tgtaactatg tcataagata agtcatagat acttatatta aaaagaatat aatagagaac
    25141 tatttttaat aattacatat taatgaaagc aaaatatttt gttatataga ttttcaataa
    25201 agcaaacaat aaatataaat gaatattaat aaggagtgct tttaattttt agaaaataaa
    25261 tgacttaaaa gaaagattta tgaaattaaa actaggattt tctgatataa taaacatgtg
    25321 tatttttaaa taaaaaggat attattatta gatttgatta aaaataaact tgcaaaacaa
    25381 tgtactagtt taatattata gttatattga ataaaatatc aggaggaagt aaaaatgaat
    25441 gtaagagtaa ggtttgcacc aagtccaaca ggatttgtac atataggtag tttgagaact
    25501 gctttatata actatttatt tgcaaaaaaa atgggcggag aatatatatt aagagtggaa
    25561 gatacagacc aaagtagatt agtagaaggt gctatagaga atatgcttaa tgctatgaaa
    25621 tgggctgggg tcaaccatag tgaaggtgtt atattagatg atagtggaaa agtagtacaa
    25681 aaaggagaat atggtccata tattcaatct caaagattag atatatacca agaatatata
    25741 aagcaacttt tagatagtgg aaaagcttat tactgtttct gtacaaaaga aagactggat
    25801 gaagtaagag atgctcaaag agcagcagga gaaacagcta aatatgatgg tcattgtaag
    25861 aatctttcta aagaagaagt agaagcgaat ataaaagcag gaataccata tgttataaga
    25921 ttaagattgc cagagaatca tacaattaag tttacagact tagttagagg agatatggaa
    25981 tttaatacta atgacttaga tgaccaagta ttaatgaaaa cagatggatt tccaacttat
    26041 cattttgcag tagttgttga tgactatttg atgaaaatta cacatgttat aagaggagaa
    26101 gaatgggtat catctactcc aaaacatgtt tatttatatg aagcttttgg atgggaagca
    26161 cctgtatttg tgcatttacc aaatatactt aataaagaaa agaaaaaatt aagtaaaaga
    26221 caaggtgatg tagctgttga agactttaag aaaaaaggat atttaccaga gggattagta
    26281 aactatgtag cattagttgg ttggtctcct gaagataatc aagaattgtt tacaatggag
    26341 gaattagaaa aagccttctc tgtagaaaga gtatctaaaa gtggtggagt atttgatact
    26401 gaaaaattaa attgggttaa tcagcactat ataaaagatg gtgatgatgc ttatctaaca
    26461 gacttagcaa taccattttt aatagaagat ggatttataa cagaagaaga agctactaat
    26521 aaatatgatt tcttaaagag tatgatatca gtacttaaag agaaattaca atatgtaaaa
    26581 gaagttactg aacatgcaag tatatttttt ggagataaaa tagaggtaga aactgaagag
    26641 ggaagagagt tcttaagatt agaacatata cctactttaa tagatgcttt gagagaaaaa
    26701 atagaaaaaa cagaggtatt aaatgctgag tttgttcaag ctatgcttaa agaaatacaa
    26761 aaagagtaca aaataaaagg gaaaaattta tttatgggct caagaataat tttgacaggg
    26821 caaatgcatg gtccagattt accaaaagta atggaagttt taggaaaaga aacttgctta
    26881 aatagaatag cttatgtaaa aaataatata ttatagtcat aaaagtccgc taatttagaa
    26941 gcggactttt tttgtgttag tatttggtga aaatatctgc ttaagaaaat gaaggtattt
    27001 aaggtaataa atttaatata taaaaagcaa agcaaaaata taaataaaat atgaataata
    27061 aactaataaa atattaagca tagaagaagg gaatctatta ttttagtttg atttaattat
    27121 taagggggta attactatgt atagtaatat tgatgatgtt aaaaaggaat taaaggagct
    27181 atgcttagaa tatgttacaa ttttagaaaa attaaaagat gaaaaaatga ttacagaaga
    27241 aacttttgaa aagtgctctt ctcaaaaaaa aatattttta gaagaacaat agaggatggt
    27301 aatagtatta ttattatgta gtttgaaaga tagtacgaaa aatgatagta atactattat
    27361 ttattgcaat tttcaactat aatataatat ataatgttaa gaagataatt tataaaaata
    27421 cagtgaggtg atgagtgtga aagtttataa tacattgact agaacaaaag aagaatttgt
    27481 tccattagaa gaaggaaagg taaaaatgta tgtgtgtggt ccaacagttt ataactatat
    27541 acacataggt aatgcaagac catttataat atttgatact ctaagaaggt atttagagta
    27601 tagaggatat gatgtaactt atgtacaaaa ctttacagat gttgatgata agattataaa
    27661 tagaagtcat gaggagggaa tatctcctga agaagttgcg gctaagtata taaaagaata
    27721 ttttgtagac tgtgatgggc taggaataaa aagagctact gttcatcctc aggttacaga
    27781 taatatacaa cagattatag agtttataaa agagttggaa gataagggat atgcatatgc
    27841 agtaaatggt gatgtatatt ttgatacaaa taagtttgaa ggttatggaa aactttcagg
    27901 tcaaaaacaa gaagatttag aggcaggggc tagaattgag gttaatgacc aaaaaagaca
    27961 tcctatggac tttgttcttt ggaaggcaaa aaaagaaggt gagccaggat gggatagccc
    28021 ttggggagaa ggtagaccag gatggcatat agaatgttct gttatgtcta agagatattt
    28081 aggtgaaaca attgatatac atgcaggtgg gcaagattta acattcccac atcatgaaaa
    28141 tgaaatagct caaagtgaag ctagaagtgg aaaaacattc tcaaaatact ggatgcataa
    28201 tggatatata aatataaatg atgaaaagat gagtaaatca aaaggtaatt tctttacagt
    28261 aagagatata tcaaaattat atgatttaga gatagtaaga tttttcatgt tatcagcaca
    28321 ttatagaaat ccagtaaact ttagtgatga gatgttaaat caagctaaag caggtcttga
    28381 aagactatat aatactaaag aaaaattaga atttacgcta agtaatttag tagaatctcc
    28441 tttaacagaa aaagaagtag agcttgttaa agaattagat gactttagac aaaaattcat
    28501 agatgctatg gatgatgatg ttaatacagc agacgcagta agtgtaatat ttgaattagc
    28561 taagttaata aattctaatg tagatgaaaa ttcttctttg gagtttgcta aaaaatgctt
    28621 agatgaattt aatgaactta caggagtatt aaatattgta aataagaaaa aggatactgt
    28681 attagataaa gacattgaag aattgataca aaaaagaact gatgcaaaga aaaataaaga
    28741 gtttcaatta gctgatgata ttagacaaca attactagat atgggaattg tattagaaga
    28801 cacaagacaa ggtgttaagt ggaaaaggat ataaaaatgg aaaaaactga attagttact
    28861 atgtcaccat tagttttagc atacttaggt gatacagtgt atgagacata tataagagaa
    28921 tatttaatta gacagaatac acaaagaaaa gttaatgatt tgcataaact agctataaag
    28981 tatgtaaaag ctaaagctca ggcaactata attcatgaag ttgaaattga gttaactgaa
    29041 gaagagagta aaatttataa aaggggaaga aatcaaaagt caaatacttc tcctaagaat
    29101 gcagatatta tagactacaa acatgcaact gggtttgagg cattagttgg atatttgtat
    29161 ttaaataatg aaatagagag acttcaatat attatcaata aaggaatcaa aattattgaa
    29221 agagatatgt aaatattaaa gagtgttgtc ttaataataa gtaataaggc aacacttttt
    29281 ttataactaa tgtataataa atatatagaa attagtaatg agaaaggata aaaagttatg
    29341 aaagtaaaat taatatcaca tacacctgaa ccagaaaaag taatagctat ggcagctaag
    29401 ctatgttatt caccagttgg gacagacgaa atagaaaaag acttaacaga tgaaagtata
    29461 gagaaatttt taaatatgtt attaagtata ggtcatgggt caatattaga acatgcttca
    29521 tttacatttt caatagaagg tatttcaagg gcatgttcac atcaaattgt tagacatcgt
    29581 atagctagtt tttcacaaca aagtcaaaga tatgttaaat tagaacaatt tgagtacata
    29641 attcctccag aaatagaaaa aatagaaaag gcaaaagagt tatttataga ctccatgaaa
    29701 aaagaccaag agaattatga taagttagtt gaaatattgt ttgaaaatca ttataatgac
    29761 ctaataaaaa acggtaaaaa tgagaaaaca gctaaaagac aagctgagaa gaaagcaata
    29821 gaagatgcta gatacgtatt tccaaatgca tgtgaaacaa agatggtatt tactataaat
    29881 gctagaagtt tgtttaattt ctttgaacat agatgctgtg aaagagcaca gtgggaaata
    29941 agaaatttag ctgtggagat gttaagagag gttaaaaagg ttgctcctat attatttaaa
    30001 aagactggtc caagttgtgt aaatggaagt tgtccagaag gaactatgac ttgtggagat
    30061 atagttcaag ttagagaaaa atttaaggcc ttgtaggagg tgaataattt ggcgagtata
    30121 gaaggaagaa atccggtaat agaagcaata aaaagcgaca gagaaataga taaaatatta
    30181 attgcaaatt cagctaaaga aggttcaatt aagaaaataa taggaatggc taaagataag
    30241 aatataatta ttcagtatgt tgataaacat aagttagatg aagtaagtac aagtcattca
    30301 catcaaggtg taatagctta tgcaagtgag tataagtatt atgaattaga tgagctaata
    30361 gatttagcaa agaataaaga tgaagaccca tttttcatta tattagatga gataacagac
    30421 ccacataatt tgggttcaat aataaggaca gcagatgcag taggagcaca tggagttatc
    30481 attccaaaaa gaaggtctgt acacataact cctgtagttg ccaaagcatc tgctggagca
    30541 gtagaatata tgccagtttg caaggtaacc aatatagtta atacaataaa aagactcaaa
    30601 gaagaagggc tgtggattgc tgcagctgat atggatggag aaacttttta taaacaaaat
    30661 cttactggtc ctcttggtgt agtaattgga agtgaaggtt ttggaatatc tagattggtt
    30721 aaacaaaact gtgactttat agtaaaaatg ccaatgatag gtaatgttac atctttaaat
    30781 gcatctgtag caggtggaat acttttatat gagatattca gacaaagact ggataaaagt
    30841 aaataggcat gaaaaaaaat ataaatcatt acttaataat agatggctac aatattataa
    30901 atgcgtggga caatttgaaa gaactggcta aagaggactt agaagattct cgagaaaagc
    30961 ttattgatga cattatagaa ttttctgagt ttatgggtta taagactata atagtttttg
    31021 atgcttataa tgttaaaaat tctcgagaaa aagttgaaaa aagaaaacac ataacaatag
    31081 tttatacaag agaacaccaa acagcagata gttatataga gaaatttata acttctttat
    31141 caaagtatga tgatgtaaaa gtagccacta atgactatgc agaacagcaa atgattttag
    31201 gaaaaggtgc tactagaatg tctgcaagag aattaaagtt agagttagat cgttccaaga
    31261 ataaaatgaa ggaaaaaaat atcggtttga ggaaaaaaat tcagcgaaat tggttggagg
    31321 aaaggttaga taaagaaaca ttgtcgaaac ttgagaacat tcgtagaaag cgttgaaatt
    31381 actaggttct ttagactata atatacctat aaatttgttt tatatatggg ggagatttac
    31441 atgttggtag caaaagaaaa aagttatgag ttagtggaca attgtcagca ggatgagtat
    31501 aacattgtat taagagcaag tgaaggggat aaaatagcac tagagtacat tattactaaa
    31561 tacagaaatt ttgtaaaggc aaaagcaaag tcatattttt taattggagc agataaagaa
    31621 gatataatac aagaaggaat gataggcctt tataaggcag ttagagattt tgatggaagt
    31681 aagaccaatt catttaaatg ttttgcggag atatgtataa caagacaaat aataactgct
    31741 ataaaaactg caacaagaca aaaacatata cctttaaatt catatgtttc tttgaataaa
    31801 ccaatatatg atgaagagtc agacagaaca cttttagata ttatagctac aagtatagta
    31861 acagatccag aagagcttat aattagtaaa gaagagttaa aaaatataga atcaaaaatg
    31921 aacgaattgt taagtgactt ggaattagaa gtactagaat tatatttaaa tggaaagtca
    31981 taccagttta tagcggacaa gcttaaaaga gatgtaaagt caatagataa cgctttacaa
    32041 agggttaaga gaaaattaga aaaacatctt gaaaacagaa atgattaata gtaaaatact
    32101 taccataagc ttattgtgag caaaattaat ttatatcgaa agataaggga ggaaattcaa
    32161 aatggctaaa gctaaatacg aaagaacaaa acctcatgtt aatataggga caataggaca
    32221 cgtagaccac ggtaaaacta cattaacagc agcaataaca aaaacattat atgacagata
    32281 tcaattagga gaagcagtag atttcgcaaa catagataaa gctccagaag aaagagaaag
    32341 aggaatcaca atatcaacag cacacgttga gtatgaaaca ccaaatagac actatgcaca
    32401 cgttgactgc ccaggacatg ctgactacgt taagaacatg ataacaggag cagcacaaat
    32461 ggacggagca atattagttt gttcagcaac agatggacca atgccacaaa caagagagca
    32521 tatactatta tcaagacaag ttggagtacc atatatagta gtattcttaa acaaatgtga
    32581 catggtagat gatgaagagt tattagagtt agtagagatg gaagtaagag atttattaac
    32641 agaatatgat ttcccaggag atgacactcc aatagtaaga ggttcagcat taat
//
LOCUS       DAYAPA010000035        32069 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.035, whole genome shotgun sequence.
ACCESSION   DAYAPA010000035 DAYAPA010000000
VERSION     DAYAPA010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 32069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32069
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.035"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            148..1362
                     /locus_tag="ACGI59_001506"
     CDS             148..1362
                     /locus_tag="ACGI59_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFG9739299.1"
                     /translation="MNQEYVKGNPIFTNINKVPRQFPYLTDDIDTDVIIVGGGVTGCI
                     CAYYLAKNNIKSVILEKGRIAHGSTSVTTSLLQYELDDNLIDLTEVMTLKDALKAYNL
                     CTSALEELDTFIELYGNKCDYAKRDTLLYTANKLEVKAIKEEYNLRKENGFDVEYIDE
                     STNPFSFDLKSGLIAKNGGRELDPYKYSHHLIDVSLKNGLQVYENTEVKKVDLSNDKV
                     TAEVSYGYKVHGKKLIVATGYNTSLFTKRNFATKSNTFNIATKPLKNIASWKNNILIR
                     DNCDPYNYLRTTKDNRLIIGGEDVSFDDIENETLANEKYDILEQRLKSMFKDIKDIEV
                     EYKYCGCFASTLDNLGFIGPDNKNNNLWYCLGYGANGILFAILGGIMLSELYLGKQNK
                     NMKLFKVDRFDK"
     gene            1539..3452
                     /locus_tag="ACGI59_001507"
     CDS             1539..3452
                     /locus_tag="ACGI59_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFG9739300.1"
                     /translation="MKYGGIIMKEILVIAPTKGTYEKSIHIVKKNKYTNIDVVFGNLK
                     EGIPLAEKSINHGTRIIISRGGTYNMLKATYNIPIVEIKVDAYDIIKSYKEVKNSNEP
                     FGIIGFNNVIYGFDIIEEILNKKITMIEIEKEEEIYDAIEKYRKKGINTYIGDTTVAH
                     IVKRLNCKGILIESREENILRAIQQAEQILEATKDEQKRRLQIEAMTDFVHDGIITVD
                     KNFKITLFNKSAEKIFGIKKENALYHNVVDVIPNTVLPQVVETMEPQIGEIQNIGSTK
                     IITNRVPIIVDGQIQGAVSTFQDSKEISSFEHTIRRSLLSKGLSAKYTFDDIIHASEK
                     TKDCIELAKKYACYDTPMIITGESGVGKELFCQSIHNYSKRKNAPFVAVNCAAIPPSL
                     IESEFFGYEEGSFTGARKKGKAGVFELAHEGTIFLDEISEIPLELQGRLLRVLQEKQV
                     MRIGGDKVIPIDVKIICASNKDLKNMMRNGLFRQDLYFRINILTLYLPSLKERKEDII
                     KLSEFFIHKYSSKYNKTPLIITPNIKKALLEREYEGNIRELEGMMERAVIVESFDSIL
                     LEKLSNEYTNNIVDKDNSDFECSNLDLKTLEKKYIYKIYNENNRNTTKTCEILKITRS
                     TLWRKLKEIEDDV"
     gene            3723..4697
                     /locus_tag="ACGI59_001508"
     CDS             3723..4697
                     /locus_tag="ACGI59_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HFG9739301.1"
                     /translation="MHILKSVKKIPGGLMVCPLLLGALFNTVCPQALEIGGFTTSLFK
                     TGAMSILALFCLCNGAQINIRQAGKPLTKGIVLTATKFIIGAVLGIIVSKFIGPKGII
                     GITPLAIVATMTNSNGGLFAALAGEYGDSTDVGAISILSLNDGPFFTMLAFGVTGLAN
                     VPIVALFAALIPILLGFILGNLDEDIREFLAPGTTLLIPFFAFPLGAALNFNQIITAG
                     LPGVVLGLGVTIITGMGGYFVMKLIRAEHPAVGAATGTTAGNAAGTPEALAAIDPTLA
                     TIAAVSTVQVAAAIIVTAICCPMLVSYLDKREKKKKSIFKTNKNIEVN"
     gene            4718..4993
                     /locus_tag="ACGI59_001509"
     CDS             4718..4993
                     /locus_tag="ACGI59_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HFG9739302.1"
                     /translation="MNAIIHANEKDNLVTCVRPLIKGEKVSIDGKSYIVNDNIPVFHK
                     MATEDVKKGDVVYKYGEIIGIATVDIKTGDYVHVHNIESTRGRGDKK"
     gene            5006..6169
                     /locus_tag="ACGI59_001510"
     CDS             5006..6169
                     /locus_tag="ACGI59_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HFG9739303.1"
                     /translation="MKLMGYLRENGQFGIRNHVLIIPTSVCSSETATRIASLVPGAIA
                     IPHQHGCCQIGSDIELTAKTLIGFGKNPNVAAVLVVGLGCDGIQAKELASEIATTGKK
                     VNYVVIQECGGTLKTVSKGAEIVSKMAREVSKEVRVEFGMSEITLALECGGSDPTSGI
                     ASNPSIGVASNLLVDEGGSSILSETTEVIGAEHLLATRFEDEEMKDKFLKFVSDVEKR
                     AIAMGEDLRSGQPTPGNKAGGLSTIEEKSLGCMYKAGNKPFKGALEYADIVPPDKKGL
                     YFMDTPGQDIDSITGMVAGGAQIVIFSTGRGTPTGSPISPVIKITGNSDTYNKMPDNI
                     DINAGRIITDGAKIADIGQEIFNEIIEVCNGKHTKAESLGHREFGIYRISSTF"
     gene            6506..8785
                     /locus_tag="ACGI59_001511"
     CDS             6506..8785
                     /locus_tag="ACGI59_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFG9739304.1"
                     /translation="MNFENTKNKKIIATLLLTLLLIVSLTCVTFIMNLNKVTHNETER
                     YLVEVSQSISTTINVKCKSIFRTLYSMGDTYFEINDSEKEVNRYLSKKLKLFEFDWLG
                     IIDSNGNFVSALKDTGNVKGQDFYRKALQGKETITPVKSQSGTYGILYSVPLYNGDKI
                     VGAVAAWNTIDTMRNLLSVESFHGEGFSQIIDQDGNFIVSSANKNAPKNVVNFFDMIK
                     HQGKQVDKNKLEEMKINLKKNKTGSMYYTLNDGISKAMHYLPLQEENLYLLSIVPTKT
                     ASAQFDLLLKKAIFINILIVILFLLLIILIMFINQKNRAQLIKIAYVDSVTKGYSKAR
                     FDIEAKKIIQSSSSESYSLVSINIQKFKLINDSFGSEAGDKTLKYIHDVISNYLEPGE
                     LLCRVSNDQFNVLIKTIPQKEILKYIENITNDINSFNKNEECKYFIALSIGVYKIDDP
                     NLPLVSIQDRANVARKNNKGNIGNHPLYTCVFYSDLERLKMIKEKEMENRMEEALNNN
                     EFIVYLQPKISLKDNTIVGAEALVRWQDPNKGLIPPDEFIPFFEKNGFITKLDHYVFE
                     KVCSMIRKWIDNGETPIPVSVNFSRANLNNIDFLEKYRMLRNKYNIPAKLLEIELTET
                     LVFENLQKLINIIDQIHEEGFQCSLDDFGSGYSSLNMLKEIKVDTLKLDRAFFSSPNA
                     DNIAENHVIESIVNLAKKLNMNSISEGVETILQMDYLKKINCDMIQGYVFSKPLPQEI
                     FEKMTFGKKLEETSMDSSV"
     gene            complement(8880..10220)
                     /locus_tag="ACGI59_001512"
     CDS             complement(8880..10220)
                     /locus_tag="ACGI59_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFG9739305.1"
                     /translation="MENQEALRHEKIWILLFRYSIPAIIAMMVTSLYNVVDRAFIGSM
                     EGIGSIAIAGLGVTMPVFTLIIAFGMLVSVGASTRLSIKLGERNREEAEKILGNALTL
                     SIIISLIITILGLVFLEDILFILGASKDSIFYAKDYMSVILVGSIFNLVAFSLNNAIR
                     AEGNPKLAARTMIVGCVLNLILDPIFIFVFNLGIKGAAIATVLCQIVVFFWVIHYFIR
                     GKSNLKLKKCNLKLNKDIVKKVFAIGMTPFAMEVAISITHMFTNNSLKVYGGDLAIGA
                     MTALTSILLMFMMPVFGLNQGMQTIISYNYGAKQYERAKKTLILSIIVSVVILSFGFL
                     VVQVFPEVFVGIFNKDSDLMDIAVRGISLNLITLPIMGISIVGPVYFQCISKVKHSMF
                     LTLLRQFILFIPLIIVLPIQFNLDGVWLAQPVADFIAMIIVLLFLKREFKTSRV"
     gene            complement(10232..11014)
                     /locus_tag="ACGI59_001513"
     CDS             complement(10232..11014)
                     /locus_tag="ACGI59_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9739306.1"
                     /translation="MNCINTKNLNVSYGNTDIIKNLNLNIPKGKITTIIGANGCGKST
                     ILKTIGRIISPNSGKIYINGEDIYKKKSKEIAKDMAVLPQSPQAPSGLTVSELISYGR
                     FPHKSGFGNSSKEDREIVNWSLEVTGIAEFKDRNMEDLSGGQRQRAWIAMALAQETDI
                     LLLDEPTTYLDLAHQLEILKLLDELNKKQGRTIVMVIHELNNAARFADHMIGIKKGKI
                     VCEGDAYDVMTKDNLKELFNIDAEIATDPRTNKPICITYDMV"
     gene            complement(11029..12051)
                     /locus_tag="ACGI59_001514"
     CDS             complement(11029..12051)
                     /locus_tag="ACGI59_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFG9739307.1"
                     /translation="MKNIIKINSKTLLVISILMVLIFITFLLSLNMGSFSIEPMDVIK
                     TFLGQGQRNHEIAIFKLRLPRIVIALLVGSALSTAGVILQGVTKNDLADSGILGINSG
                     AALFVVVYIYLMNGNVYEGMSNLTVFTMPIVALIGALFGAFLIYSLAWKGGINSSRLL
                     LIGIGVNIAFTSILTIFQLKFTTQEFNRVMAWTSGSIWGTSWKYVIAVLPFIVIFMGI
                     TIYKSKYIDVLNLGDEVSISLGVDVEKQRRRLIIYAVILSGVSTAVAGSISFLGLIAP
                     HIGRKIIGPKHKRLIPVSALIGTLLLLIADTISRNLLAPIEIPVGIVVSIIGVPYFIY
                     LMLVND"
     gene            complement(12057..13070)
                     /locus_tag="ACGI59_001515"
     CDS             complement(12057..13070)
                     /locus_tag="ACGI59_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFG9739308.1"
                     /translation="MTKLSKKNITYLLILVSIVVLVFGIVLSITIGAKDMNLSTVIDS
                     LIKMEDGINMRIVKDVRLPRAIAAALVGGFLAVSGAIMQGITRNPIAEPSVIGITQGA
                     TFAISISLVLQKKLPQLIHGSFSVMMFAFIGASISGLFIYFISSKSRGRVNNVKLALA
                     GVALGTLLISLASAISMYFNLSQQLSFWISGGLVGVKWEGIKLLFVAGGIGFILAIIM
                     APRITILSLGEEVAIGLGQKTNFVRFICIVLVILMTGASVSVSGNIIFIGLIVPQIAK
                     GIVGSDYKYIIPSSLVLGAVLLVYTDILSRMINPPYETPVGSITALIGVPIFIYLVRK
                     GEK"
     gene            complement(13198..14136)
                     /locus_tag="ACGI59_001516"
     CDS             complement(13198..14136)
                     /locus_tag="ACGI59_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFG9739309.1"
                     /translation="MKKIKSLAIFISIITTLVLVTACSDKNTEDKDKSETRVVQSVKG
                     EVKIPSNPKKIVDISGSSEELLLAGYKPVATANVDSYETDKLPSYIREELKGVKIVGH
                     SMMDTMDMEAILEVNPDLIIMSQRQEKIYDQLKEIAPVVMMKDYANDWRSKLTDVSKL
                     FDKEEEAKSWLQKYDEKATKLGKEVIEKNGEKTYLPVLASSGQFMVFSDGGIGTLIND
                     DMKLAKPKNMPKQDGITLPMVSMEGLTDIDADHIVVIATEADKKDLENSAIWAQIRAV
                     KDGNVTILDAAPFFSQSYNPIGKELLLESVKNELTK"
     gene            14406..14870
                     /locus_tag="ACGI59_001517"
     CDS             14406..14870
                     /locus_tag="ACGI59_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016208838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9739310.1"
                     /translation="MVHLVYCDNAGKKGEKVLDKILAGTKTMVIRGAAGRKIPHSRVF
                     EDELLYFMEKGTAKISAKATVKKVENYVKLTEDEISKILNENQPKLNLSEKQKVRWHK
                     KCLCLVEFENVEEITPLDFDHQGNMDDWLIVEKIEDVVVGTSIPYNYEKSKF"
     gene            complement(14982..15299)
                     /locus_tag="ACGI59_001518"
     CDS             complement(14982..15299)
                     /locus_tag="ACGI59_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFG9739311.1"
                     /translation="MDEKMIEISFGIIGFAGDAKGLAFEAIKEAKSGNIDKARECIEK
                     SKESIHKAHKFQTELIQNEANGNKTEISVILIHAQDHLMTAMNFQQLAEEFIDVYERL
                     ESK"
     gene            complement(15312..16043)
                     /gene="chbG"
                     /locus_tag="ACGI59_001519"
     CDS             complement(15312..16043)
                     /gene="chbG"
                     /locus_tag="ACGI59_001519"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HFG9739312.1"
                     /translation="MTKIIINADDFGYCEAVNYGIISAHNNGIVRSTSMMANMPGVEH
                     GVGLLKENKTLNCGVHMTLSCGRPLLSNLKTIVDKDGFFIRRITDEIIEKMDCDEIYR
                     ELCAQIDRVKGLGIDISHLDSHHHIHTLLSLKPVIEKIVTKYNLPIRGGFEYNLEYSK
                     VVPLIDSFYKENVSEEYFIKNIDEIMKYDVVDIMSHPAFLDDYILNSTSYAIDRTKEH
                     KILTSKKVKEFLEKNGLVISSYRDI"
     gene            complement(16069..17379)
                     /locus_tag="ACGI59_001520"
     CDS             complement(16069..17379)
                     /locus_tag="ACGI59_001520"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFG9739313.1"
                     /translation="MKKGLKIVTIGGGSSYTPELVEGFINRYEELPVKELWLVDIEAG
                     KEKLEIVGNLAKRMVKKAGVDMKINLTLDRREALRDADFVTTQLRVGLLDARIKDETI
                     PLSHGVMGQETNGAGGLFKALRTIPVIFDIIKDVEELCPNAWMVNFTNPTGIITEAVF
                     KYTNFRRYIGLCNVPIHLKNDVAKLFNVESDRISMDFAGLNHMVYGLNVSLDGEDVTK
                     EAIDKFVKADISMQNIKAIDFNAEFVKSLGAIPCPYHRYYYKTKEMLEDELREFKEGK
                     ARGQVVKELEEQLFELYKDEKLDVKPPQLEKRGGAYYSDAACNLISSIYNDKKDIQVV
                     NTLNKGSIRDFKDEQAVEVSSVITKNGPKPISIGYLPDSVHGLVSQIKSFEVLAAKAA
                     VYGDYESALLALCINPLIPSDDLAKTILDEMLEAHKDYLPRFNR"
     gene            complement(17476..18789)
                     /locus_tag="ACGI59_001521"
     CDS             complement(17476..18789)
                     /locus_tag="ACGI59_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFG9739314.1"
                     /translation="MEKFMSFMDKYIVPVAAKIGAQRHLVAVRDAFIVMIPITMVGAL
                     GTLINNLPLEAYKNLMASIFGENWTTFGGDLWWGAIGTMAVFLVIGVAYFLAKSYESD
                     GLQSGLIALSIFFIMAPQIGKIVPEGGTTVVEGWGMIQQTYLGTAALFSSILIGLLST
                     EIFVRLSKVKKLTIKMPDGVPPAVSRSFAKLIPGMLTIMIFTVIGIFIKMLSNGSFLT
                     DILNTYLGAPLSNVADSLGSTMLIAFIIHILWTVGLHGANIALPFTETILMKLGGENA
                     ALAQAGATEGYHVLAGAFFDAFVYLGGSGMVLGLIVALLIAGRRRKEMIVLGGPPAIF
                     NIGEPLIFGLPIVLNPIFMIPFVLAPVICSAVSYLAIDFGLVAPVILPKIPWVTPPIL
                     GGAMATGDWTGGALALFNLILSILIYIPFVIASEKMEANKLKINN"
     gene            complement(18811..19122)
                     /locus_tag="ACGI59_001522"
     CDS             complement(18811..19122)
                     /locus_tag="ACGI59_001522"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFG9739315.1"
                     /translation="MIKIMLACSAGMSTSLLVTKMEDAAKENGIEAKIWAISEVNLKN
                     EIENCDVLLLGPQVRYVLGKATEMAKPYNIPVEVINMMDYGRCNGKAVLDRAVELNSS
                     K"
     gene            complement(19322..20026)
                     /locus_tag="ACGI59_001523"
     CDS             complement(19322..20026)
                     /locus_tag="ACGI59_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFG9739316.1"
                     /translation="MKEPIYKVIENHVRELINSDSLKEGDLIPSEKQLSEEFNVTRMT
                     VRSALNNLVKEGYITRQRGVGSIVIANNIYDNISSVSGFTKEMESKGYKVSNILVSLE
                     IVQADENLSSKLNISLEENVWEIKRVRLANDVRVSYMITYMPVKLFPNLNKTHCENSL
                     YNFVEEVCGYKIAMSEREVQAVISNEECMDNLKLEEPEPLLYISQICKLQNSEIFEYS
                     HTYHYGYTLTLNAVVE"
     gene            complement(20117..21478)
                     /locus_tag="ACGI59_001524"
     CDS             complement(20117..21478)
                     /locus_tag="ACGI59_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFG9739317.1"
                     /translation="MKDRLNLTEGRITEKLLKLSLPIMGTSFIQMGYNMIDMIWVGKA
                     GSKAVAAVGTAGFFPWLAMAFIMISKVGGEIKVAQSIGANNISTTKSYMKSAIEINII
                     LAIIYTISLIVLNKQLIGFFRLGDLEVITMSRQYLIIVALGMVFYFINPVFTAIFNGL
                     GNSKTPFRINTVGLITNIILDPILIFGWGFAPKLGVAGAAIATVFAQIVVTTCFIYII
                     VKSKEEYFKLRLFKEIETKYYKVLYKLGLPIAIQSGMFTVFSMLLGVIVASWGPVAVA
                     VQKVGSQIEAISWMTAEGLSVALGSFVGQNYGAKKYSRINKGCKIAITVSSVLGIITT
                     LVLVFAGESIFSIFIDESEAIEKGAVYLKILGYSQFFMCLEIITTGAFKGLGRTYIPS
                     IIITILTGARVPLAYFISRPEMLGLNGVWWSITLSSILKGILMISLFISLLKLGKLYK
                     ISE"
     gene            21977..23131
                     /locus_tag="ACGI59_001525"
     CDS             21977..23131
                     /locus_tag="ACGI59_001525"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFG9739318.1"
                     /translation="MKINKKFNRVDMQVSILTAIIVIISSSCVYFFNYTLTYKSMIYS
                     LSERSKHIYEYVERNIDKNTFTEINSKEDQSKKSYKSSKILLESVKNTTGVMYLYTAK
                     KTKDGSLVYVVDGLNSSRSDFRNAGDLIEKEIWGDLNKALDNHILLPKKIKSTSWGYI
                     FISYFPIHNDVGKVVGVIGIEFEAKHQYNTFKFISIVTPIIALLTCLISALIAVILFK
                     RISNPTYTDFANTDYLTGLKNRNAFEIDMNNLNNSNLKNLISIISIDLDGLKKINDKF
                     GHQTGDLYIKQASKIIQYVINKKYIVYRIGGDEYIIILKNLSHKEINNIINNINLKIV
                     EENSTNELKLSMSIGYAIFDEKVDVSLFDTYRRADSQMYDNKRKINKQYQ"
     gene            complement(23213..24931)
                     /locus_tag="ACGI59_001526"
     CDS             complement(23213..24931)
                     /locus_tag="ACGI59_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033225 - ATPase-coupled
                     2-aminoethylphosphonate transporter activity [Evidence
                     IEA];
                     GO_process: GO:0033223 - 2-aminoethylphosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     permease subunit"
                     /protein_id="HFG9739319.1"
                     /translation="MNNNAISSKSISLKSKMNLDIKKCMLTVLVMSLVIFLLAPIIYL
                     FVQAFLDKDGVFVGLQNFQEYLASESLLNSLKNTVFVSVISTVISLTLALVYAYALTR
                     TKIKFKGFFKSLAMFPMFAPTMMHAIGLVYLFGNQGLVTNLLGVHVPIYGAVGIILAQ
                     IIYVFPQSFQILYISLSSTDYRLYEASNTMGISKIREFISITIPSIKYSLISSAFVSF
                     TLCFTDFGAPKVIGGEFNVLSIDVYKQIIGQQNMPMGATVSILLLIPAIISFIVTKKI
                     EKNQNSYITSKSIPYKVKTNKLRDSILGGVVGIIAFGIIAVMLTVILSSLISVWPYDL
                     RLTLEHFSMKNSIDGIKPFINSIKMSLLTAILGTSFVFLFAYLNEKTEKAPYLKSFGY
                     FLSTLPMALPGLVLGIAYVLFFNKLEFNFMDTIYIKNLFNGLYGTLLIMVICNIVHFF
                     SVAFITATTAIKKVDKELDMVAKSMGINSASILKNVTIPLCLPAVLENFMYFFLNSMV
                     TISALVFIYTASSKVAAISIVQLDDKGSTAQAASLAVLILVTNILVKVIYEFIKNRLE
                     KRTNFN"
     gene            complement(24936..25445)
                     /locus_tag="ACGI59_001527"
     CDS             complement(24936..25445)
                     /locus_tag="ACGI59_001527"
                     /inference="COORDINATES: protein motif:HMM:NF019998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HFG9739320.1"
                     /translation="MSALDAKVRHKLRMDIKRLQKELNITTIMVTHDQEEAITMADKI
                     AILNGGDIMQIGTPEEIYQNPQNLFTAQFIGDTNCFDNGDSILTVRPEYVQIEKSTKE
                     NYQGIISNIEFRGNLLRVEIKDKLNENFIISDASIKEWVNLNLVEGDLVKISIDEKYY
                     LKYPKVKGA"
     gene            complement(25455..25805)
                     /locus_tag="ACGI59_001528"
                     /pseudo
     CDS             complement(25455..25805)
                     /locus_tag="ACGI59_001528"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     regulatory      26094..26191
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            26246..26422
                     /locus_tag="ACGI59_001529"
     CDS             26246..26422
                     /locus_tag="ACGI59_001529"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9739321.1"
                     /translation="MFRDKMDKCTHVLTAYIGSSYDYCDFIDTQLDDFILEYGEKVVE
                     SCLHQVMVLVSKYN"
     gene            26529..26729
                     /locus_tag="ACGI59_001530"
     CDS             26529..26729
                     /locus_tag="ACGI59_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9739322.1"
                     /translation="MCFKTYILKRVVNNKQLEITRSFELDNLICTATEILKDDEIDLI
                     IIDEKNSIVWRNEKSTPQQENA"
     gene            complement(26908..27780)
                     /locus_tag="ACGI59_001531"
     CDS             complement(26908..27780)
                     /locus_tag="ACGI59_001531"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9739323.1"
                     /translation="MSNINNKNKSWIEKHKILTIFISVIAILIICIGLPSLINRVLYW
                     DIKTHNSTDGEWLSFWGSFLGGIFGGLATLGAILITREMAKKDNEELKESISMEREPK
                     VIPIKKNAYLYKTYGSPCMFNEIKTSSEVPRCSDIIELTLVNVGKEAAIQIEVFWEKP
                     LNIVKTEYLTDDDLEVCNNFFDEFDENENIKVEKEYISSLQEVEIGLNAYTEMYIREL
                     SSKLIDNFRENNEDGIYIRKNIKAGTINIKCKNIYDKSYNKSYDMEISIFEMDRSKEL
                     YTINIDFNLIEDEG"
     gene            complement(27977..28561)
                     /locus_tag="ACGI59_001532"
     CDS             complement(27977..28561)
                     /locus_tag="ACGI59_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9739324.1"
                     /translation="MKGEYNKEKEKVIKFNSNKDKSKINKYTDYYNTLNSTLMEKNKC
                     IGDNWFLATTSEEVGNMNEEEKRILETEKRILETEKRVHQNNKEVKESIDKQFNDIKG
                     IFKEYKNDLDKDRIELKNDLARERIELKSDLKEAINEHKKVTEKDVSEIKNSIKGIED
                     RIDSTNKWIIALCITTIIGIATMAITIGISIWPK"
     gene            complement(28564..29055)
                     /locus_tag="ACGI59_001533"
     CDS             complement(28564..29055)
                     /locus_tag="ACGI59_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9739325.1"
                     /translation="MAYENGTNLVAYFPNCIKKYEKDNKIFEKTILLLNLYMQMPTWP
                     HCIHEFKAKANEILGEDPEFIGEYGIKLKDFYNEGENVNISQNFKNDLYTFSNSTYDI
                     LDNSYIQRINPIGLYSISSLARENDFILKLFRNDKKSLELELSKSEIQSIIEHLESMI
                     EEE"
     gene            complement(29305..30111)
                     /locus_tag="ACGI59_001534"
     CDS             complement(29305..30111)
                     /locus_tag="ACGI59_001534"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFG9739326.1"
                     /translation="MKIAIVPGHTLSGKGTGATGYIDEGKENRILTDLIVKWLKQGGA
                     IVYTGKVDKSNNYLAEQCKIANRQNVDLAVQIHFNANKTTLNPMGTETIYKTNNGKVY
                     AERVNSKLSTVFKNRGAKSDVRGLYWLSHTKAPAILIEVCFVDSKADTDYYIRHKDIV
                     AKLIAEGILNKTIDNKENGEGKIMYKHTIVYDGEVDKIPATVVGWGYNDGKILICDIK
                     DYVPGQTENLYVVGGGACEKISSMTKERYTMIKGNDRFDTLYKALEFINK"
     gene            complement(30111..30368)
                     /locus_tag="ACGI59_001535"
     CDS             complement(30111..30368)
                     /locus_tag="ACGI59_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFG9739327.1"
                     /translation="MDNLISFIPEQLLLLVVALNVLGFGFKKYKQLDNKYIPIILLVI
                     GIGFSIWMLGFNPSSILQGILCWGVAIGANQVYKQLKDGDK"
     gene            complement(30388..30618)
                     /locus_tag="ACGI59_001536"
     CDS             complement(30388..30618)
                     /locus_tag="ACGI59_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin XhlA family protein"
                     /protein_id="HFG9739328.1"
                     /translation="MNEELVKEKISTLETRVKEHGKQIDRIEIEQAKFAIQIQNLCSD
                     IKNLTGVLKWLVGVIITTLVGFFIFAIQKGIF"
     gene            complement(30659..30841)
                     /locus_tag="ACGI59_001537"
     CDS             complement(30659..30841)
                     /locus_tag="ACGI59_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015981517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9739329.1"
                     /translation="MNINNVVVRILAERILNKGLNPLKNRVFELDDVTNTEYRMAVEN
                     YIIEHSEVVEGAELTK"
     gene            complement(30841..31134)
                     /locus_tag="ACGI59_001538"
     CDS             complement(30841..31134)
                     /locus_tag="ACGI59_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9739330.1"
                     /translation="MERGNRIICDQTGKILLQTGEATGDILEHDEITELHFVDIPYGS
                     IDYTRNRIIGINIETKEPILEEIPTFISDEEKRIQELENQILLNENEKVGGIL"
     gene            complement(31155..>32069)
                     /locus_tag="ACGI59_001539"
     CDS             complement(31155..>32069)
                     /locus_tag="ACGI59_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9739331.1"
                     /translation="KITLLQALPFLTVSSYNSITATFKADHTDPTLLTKIEYFIDDVL
                     KETITTDLTTEKTINYTLEDNALHTLKIVVTDSNNATAEKVLSISKNIMPLPEDVNLQ
                     DISSKLIEVNAGFKTGKTSIINTLALKNIEANLNNTLVELSEKIKTGFDSSDATAEQL
                     QNQITNLNNQLSQRKRWAKGTYTFTQSDYENFYSRYSSAENYAKSLTVPINLNFIPSL
                     VIVEKLCFYGNGYTVNFYAINNLNQLASVGRVPKSSGEYRFCAFMFVKTITSKEFSLY
                     TYRDEYTDSSSVVYRCKVGESFTWYAYE"
BASE COUNT        11172 a         5112 c         4062 g        11723 t
ORIGIN      
        1 tatgaatata ttatacatat gttttatact aaaatgttat cacattttat ctatacatac
       61 ccatataact agataattaa aatgtataat attatttgct atagaaaata atacactcat
      121 acataataaa ttttaggagt gaagtatatg aatcaagaat atgtaaaagg aaatcctatt
      181 tttactaata ttaataaagt tccaagacaa tttccatacc ttactgatga tattgatact
      241 gatgttataa ttgttggtgg tggcgtaact ggatgtattt gtgcttacta tctagctaaa
      301 aacaatataa agtctgtcat tcttgaaaaa ggaaggattg cacatggaag cactagcgtg
      361 acaacttctc tattacagta tgaattagat gacaatttga tagatttaac agaggttatg
      421 actctaaaag atgctctaaa agcatacaac ctttgtactt cagcacttga agaattagat
      481 acatttatag agctatatgg aaataagtgt gattatgcca aaagagatac tctactttat
      541 actgccaaca aacttgaagt taaggctatc aaagaagaat ataacttaag aaaagaaaat
      601 ggatttgatg ttgaatatat tgatgaatcc acaaaccctt ttagctttga tttaaaatca
      661 ggattgatag caaagaatgg aggacgagaa ttagacccat ataaatatag tcaccatttg
      721 attgatgtaa gtttaaaaaa tggtcttcaa gtatatgaaa atacagaagt aaaaaaagta
      781 gatttatcaa atgataaggt tactgctgaa gtatcgtatg gatacaaagt tcatggtaaa
      841 aaattaattg tagctacagg gtacaataca agtttattta caaaaagaaa ttttgcaact
      901 aagtcaaata cttttaatat agcaacgaaa ccacttaaaa acatagcttc ttggaaaaac
      961 aacattttaa ttagagataa ctgtgaccca tataattatc taagaactac taaagacaat
     1021 cgattaatta taggtggtga agatgtaagt tttgatgata tagaaaatga aactttagcc
     1081 aatgaaaagt atgatatttt agaacaaaga ttgaaaagta tgtttaagga tataaaagac
     1141 attgaagttg aatataaata ttgtggttgt tttgcttcaa ctttagataa tttaggtttt
     1201 attggtccag ataataaaaa taacaatcta tggtattgct taggttatgg agcaaatggt
     1261 atactctttg caatattagg aggtataatg ttaagtgaat tatatcttgg caaacaaaat
     1321 aaaaatatga aattatttaa agtagataga tttgataaat agatattata tattaaaaga
     1381 ctgtaaaaag atgaaacttt ataattctaa aagttaactt tgatacagtc ttttataatg
     1441 ttccttttaa aaatacttta tttataatta aatataaagt aaaatgttaa aatataacta
     1501 ataaaattta gtcataagat aatatttaag tttatttgat gaaatatgga ggaattatta
     1561 tgaaggaaat actcgttatt gctcccacta aaggaaccta tgaaaaatca attcacatag
     1621 ttaaaaaaaa caaatataca aatattgatg ttgtatttgg caatctaaaa gaaggtatac
     1681 ctcttgctga aaaaagtatt aatcatggta ctagaattat cataagccga ggtggaactt
     1741 acaatatgtt aaaagctact tacaatatac ctatagttga aattaaagta gatgcttatg
     1801 atattataaa aagctacaag gaggtaaaaa attccaacga accatttgga ataattggat
     1861 ttaataatgt catctacggt ttcgatatta tagaagaaat attgaataag aaaataacta
     1921 tgattgaaat tgaaaaagaa gaagaaattt atgatgctat agaaaagtac agaaagaaag
     1981 gtataaacac atatatagga gataccactg ttgcacatat tgttaagaga cttaattgta
     2041 agggaatatt aatcgaatct agagaagaaa acatacttag agccattcaa caagcagaac
     2101 aaatactcga agcaaccaag gatgaacaaa aaagaagatt acagatagaa gctatgacag
     2161 attttgtaca tgatggaata ataacagttg ataaaaattt taaaataaca ttatttaata
     2221 agagtgctga aaaaatattt ggtataaaaa aagaaaatgc actttatcat aatgttgtag
     2281 atgttatccc aaacacagtt cttccacaag tagttgagac tatggaacct caaattggtg
     2341 aaatacaaaa tatagggagt acaaaaatta ttacaaatag agttccaata attgttgatg
     2401 gtcagattca aggggctgtt tcaacatttc aagatagcaa ggagataagt tcttttgaac
     2461 atacaataag aaggagctta ttaagcaaag gtctatctgc taaatacact tttgatgata
     2521 taattcatgc tagtgaaaag acaaaagatt gtatagaact tgcaaaaaaa tatgcttgtt
     2581 atgatactcc tatgattatt actggagaat ctggagttgg taaagaacta ttttgtcaaa
     2641 gtatacataa ctatagtaag cgtaaaaatg caccttttgt tgctgtaaat tgtgctgcca
     2701 tacctccatc tctaatagag agtgagtttt ttggttatga ggaaggttca tttacaggtg
     2761 ctagaaaaaa aggtaaagca ggtgtattcg aactagcaca tgaaggcaca atcttcctag
     2821 atgaaataag tgaaatccct cttgaacttc aaggtcgatt attgagggta ttgcaggaaa
     2881 aacaagttat gagaattggt ggtgataaag ttattcctat agatgtaaaa attatctgtg
     2941 cttcaaataa agacttaaaa aatatgatgc gcaatggttt atttagacaa gacctttatt
     3001 ttagaataaa tatactaaca ttatatcttc cttcattaaa agaaagaaaa gaagatatta
     3061 taaaattatc agaatttttt atacataaat attcaagcaa atacaataaa acgcctttaa
     3121 ttatcactcc aaatattaaa aaggcacttc ttgaacgaga atatgaaggt aatataaggg
     3181 aattggaagg tatgatggaa agggctgtta ttgtggaatc atttgactcg attttattag
     3241 aaaagcttag caatgaatat acaaataata tagttgataa agataatagt gattttgaat
     3301 gcagtaattt agatttaaaa actttagaaa aaaaatatat ctataaaatt tataacgaaa
     3361 ataatagaaa tactacaaaa acctgtgaaa ttctaaagat aactcgctcc actctatggc
     3421 gtaagttaaa agaaatagaa gatgatgttt aaaaataata catatctata tatatcaaaa
     3481 cgttttatgt ttaattttaa tacataagac gtttatttgt tttcaataaa aaatacaatt
     3541 atatgctaaa aagaatcttt gtaagtgaaa ttctcgtcaa tcatactttt aattataagt
     3601 aatctacagc atttcaatcg attattaaaa ataatcatga taactaaaga tacaaactct
     3661 atatattggc atagtaattg cttttataat acctatataa aaatgaaagg gagtgatact
     3721 agatgcacat cttaaaatca gtgaaaaaga taccaggagg acttatggtc tgtcccttgt
     3781 tattgggagc actatttaat acagtatgtc cacaagctct tgaaattgga ggattcacaa
     3841 catccttatt taaaactggt gcaatgagta tacttgctct attttgtcta tgcaatggag
     3901 ctcaaatcaa tatcagacaa gctggtaaac cattgactaa aggtatagta cttacagcaa
     3961 caaaatttat aattggtgca gtattaggta ttattgttag taaatttatt ggacctaaag
     4021 gtataattgg tattacacct cttgccatag ttgctaccat gactaattca aatggaggtc
     4081 tatttgctgc tcttgcaggt gaatatggag actccacaga tgttggagca atatctatct
     4141 tatctctaaa tgatggtcct ttttttacaa tgcttgcttt tggagtaact ggacttgcaa
     4201 atgtacctat agtagcacta tttgcagcac ttataccaat actacttgga tttatacttg
     4261 gtaaccttga tgaagatata cgagaatttc ttgcacctgg tacaacatta ttaattcctt
     4321 tcttcgcttt tcctttagga gcagcattaa actttaatca aattataact gctggactac
     4381 ctggagttgt acttggttta ggagttacta taataacagg tatgggtggg tactttgtaa
     4441 tgaaacttat acgtgcagaa catccagcag taggtgcagc tacaggtaca acagccggaa
     4501 atgcagctgg tactcctgaa gctttagcag ctatagaccc tactctagct acaatagcag
     4561 ctgtttcaac agtacaagtt gctgctgcta ttattgttac agcaatatgt tgtccaatgt
     4621 tagtttccta tctagataaa agagaaaaaa agaaaaaatc tatttttaaa acaaataaaa
     4681 atattgaagt aaattaatac ttaaggaggt aaaattaatg aatgcaatta ttcatgcaaa
     4741 tgaaaaagat aatctagtta catgtgtcag acctttaatt aaaggtgaaa aagtttcaat
     4801 tgatggaaag tcttacatag taaatgataa tatacctgtc tttcataaga tggctacaga
     4861 agatgtaaaa aaaggtgatg ttgtatacaa atacggtgaa attataggaa ttgcaacagt
     4921 tgacataaaa actggtgact atgttcatgt acataacatt gaaagtacac gtggtcgtgg
     4981 agataaaaaa taggaggaag ttaaaatgaa attaatgggt tatttaagag aaaatggtca
     5041 gtttggtatt cgtaatcatg ttttaattat tccaacatct gtatgttcaa gtgagacagc
     5101 tacaagaata gcatcacttg tgcctggagc aatagcaatc cctcatcaac atggatgttg
     5161 tcaaattggt tctgatatag agcttacagc aaagacatta ataggatttg gaaaaaatcc
     5221 taatgttgcc gcagttttag tagttggtct tggatgtgat ggtattcaag caaaagaatt
     5281 agcatctgaa atagctacaa caggaaaaaa ggtaaattac gttgttatac aagaatgtgg
     5341 tggaacatta aaaacagttt ctaagggagc tgaaatagta agtaaaatgg ctcgtgaagt
     5401 atcaaaagaa gtaagagttg aatttggtat gagtgaaatt acattagcac ttgaatgtgg
     5461 tggttctgac ccaacaagtg gaatagcttc aaacccttca ataggcgttg catcaaacct
     5521 tcttgtagat gaaggtggaa gtagcatact aagtgaaact acagaagtaa ttggtgcaga
     5581 acatttgttg gctacccgtt ttgaagatga agaaatgaaa gataagtttc ttaaatttgt
     5641 aagtgatgta gaaaaaagag caattgctat gggagaagat ttacgtagtg gtcaacctac
     5701 tccaggcaat aaagctggtg gactttcaac aattgaagaa aagtcacttg ggtgtatgta
     5761 taaggcaggc aataagccat tcaaaggtgc tcttgaatat gctgatattg tacctcctga
     5821 taaaaaagga ttatatttta tggatactcc aggtcaagat atagattcta tcactggtat
     5881 ggtagcaggt ggtgcacaaa ttgtaatctt ttcaactggt agaggtactc caacaggaag
     5941 ccctatttca ccagttatta aaatcactgg gaatagtgat acatacaaca aaatgccaga
     6001 caatatcgat ataaatgcag gtagaattat aacagatggt gctaaaatag cagatatagg
     6061 tcaagaaata tttaatgaaa taatagaagt ctgtaatggt aaacatacca aagctgaaag
     6121 tcttggtcat agagaatttg gcatttacag aatttcatct acattttaga ttgtaacaaa
     6181 atatctttta taaatataat acttatgtag atacataagc taaaagttct ttctaatact
     6241 ataaaaattt tctatactga aaaggaactg tatcaaaatt attttgaaaa cagttccttt
     6301 tttaacctct tttactcaaa atattataaa ataagattta aacaataaac gtatcttgat
     6361 taatctctaa gaagacataa ataaagctaa atgtaaagtg ggataaataa aatttaatat
     6421 ctttgtatga aaagatattt ggtataatat gtctgagaga attcttattt gtaaacaaat
     6481 aaaggatgga ggatgaaaca aaagaatgaa ctttgaaaac acaaaaaata aaaaaataat
     6541 agctacttta ctattgacac tccttttaat cgtgtcatta acatgtgtca cttttattat
     6601 gaatctaaat aaagtaacac ataatgaaac agaacgatat cttgtagaag tttcacaaag
     6661 tatttccact acaattaatg tgaaatgtaa aagtatattt aggacactat attctatggg
     6721 agatacttat tttgagatta atgatagtga aaaagaagtc aatagatact tatcaaaaaa
     6781 attaaaatta tttgaatttg attggttagg tattatagat tctaatggaa attttgtaag
     6841 tgcccttaaa gatacaggta atgttaaagg acaagacttt tatagaaaag cccttcaagg
     6901 caaagaaaca attactcctg ttaaatcaca atctggtaca tatggaattt tatattctgt
     6961 accactatac aatggtgata aaattgttgg agctgttgct gcttggaata ctattgatac
     7021 tatgagaaat ttattaagtg tagaaagctt tcatggtgaa ggattttccc aaattatcga
     7081 ccaagatgga aattttattg taagttcagc aaacaaaaat gctccaaaaa atgtggtaaa
     7141 tttttttgac atgattaaac accaaggaaa acaggttgat aaaaataaac ttgaagaaat
     7201 gaaaataaac ttaaaaaaga acaaaactgg ttctatgtat tatacattaa atgatggtat
     7261 ttctaaggca atgcattatc ttccattaca agaagaaaac ttatacctcc tttcaattgt
     7321 tccaacaaaa acagcaagtg cacagtttga tttacttcta aaaaaagcaa tcttcatcaa
     7381 tattttaatt gttatactat ttttattgct tattatattg attatgttta taaatcaaaa
     7441 gaacagggca caacttataa aaatcgccta tgtagactct gtaactaaag gttacagtaa
     7501 agcacgtttt gatattgaag ctaaaaaaat tatacagtca tcttcatcag aaagctattc
     7561 attggtatct ataaatattc agaaattcaa acttattaat gattcttttg gtagtgaagc
     7621 aggagataaa acattaaagt acatacatga tgtcattagt aattatctag aaccaggaga
     7681 attgctttgt cgagtttcta atgaccagtt taatgtactt attaaaacta ttcctcaaaa
     7741 agaaattctt aagtacatag aaaatatcac caatgatatt aatagtttca ataaaaatga
     7801 agaatgtaaa tattttatag ctctatctat aggtgtatat aaaattgatg acccaaatct
     7861 acctttagtt tcaattcaag accgtgcaaa tgttgcacga aaaaataata aaggaaacat
     7921 tggaaatcat ccactttata catgtgtttt ttatagtgat ttagagcgtt taaaaatgat
     7981 taaagaaaaa gaaatggaaa atagaatgga ggaagcatta aataataacg aatttatagt
     8041 atatcttcag cctaaaataa gcctcaaaga taatactatt gtaggtgcag aagcattagt
     8101 aagatggcaa gacccaaata aaggtcttat accaccagac gagtttattc ctttctttga
     8161 aaaaaatggt tttattacaa aactcgacca ctatgttttt gaaaaagtat gcagtatgat
     8221 aagaaaatgg atagataatg gagaaactcc aatacctgtt tcagttaatt tttcacgtgc
     8281 taatttaaat aatatagatt ttttagaaaa atacagaatg cttcgtaata aatacaacat
     8341 tcctgctaag ctcttagaaa ttgaattaac tgaaacactt gtatttgaaa atctccaaaa
     8401 attaattaat ataatagacc aaatccatga agaaggtttt caatgttcac tagatgactt
     8461 tggaagtgga tactcttcac tcaatatgtt aaaagaaata aaagttgaca cattaaaact
     8521 tgaccgtgct ttttttagct cacctaatgc agataatatt gctgaaaatc atgtaataga
     8581 atctatagtt aatttggcaa aaaaattaaa tatgaatagt atatcagaag gtgttgaaac
     8641 aatcttgcaa atggattatc ttaaaaaaat taattgtgat atgatacaag gatatgtttt
     8701 ttcaaaacct ctacctcaag aaatatttga gaaaatgact tttggaaaaa aactagaaga
     8761 aaccagtatg gatagttccg tttaaataaa ataaaaaact tgtctctata aaataatttt
     8821 aatcatttta taaagacaag tctttttatt ttccaattta attttatttt ctaaatttct
     8881 tacactcggc ttgttttaaa ttcccttttt aagaataata atacaattat catagcaata
     8941 aaatctgcaa ctggctgtgc caaccaaaca ccatctaaat taaattgaat tggaagtaca
     9001 atgattaatg gtataaataa aataaattgt cttaaaagag ttaaaaacat tgaatgttta
     9061 actttactta tacactgaaa atacacaggt cctacaatag aaatccccat tataggcaat
     9121 gtaattagat ttaggcttat acctcttact gctatatcca tcaaatctga atctttatta
     9181 aatataccta caaatacttc tgggaaaact tgtactacta aaaatccaaa tgacaatatg
     9241 acaactgaca ctatgataga taatattaaa gttttctttg cacgctcata ttgttttgca
     9301 ccataattgt atgatattat agtttgcata ccttgattca atccaaacac aggcatcata
     9361 aacatcaata aaatagatgt aagtgctgtc atagctccaa tagctaaatc tccaccataa
     9421 acctttagag aattatttgt aaacatgtga gttatactta tagctacttc catagcaaaa
     9481 ggtgtcattc ctattgcaaa taccttcttt actatatctt tattcaattt taagttacat
     9541 ttttttagtt ttaaattgga cttccctctt ataaaataat gtattaccca aaagaatact
     9601 actatttgac agagtactgt tgctatagct gctcctttta tacctaaatt aaatacaaat
     9661 ataaatatag ggtccaatat caggtttaat acacatccta caatcattgt ccttgctgct
     9721 aattttggat taccttctgc cctaattgca ttattaagag aaaatgcaac taaattaaat
     9781 atacttccaa ccaatatgac actcatatag tcttttgcat aaaatattga atctttactt
     9841 gcacccaaaa taaaaagtat atcttctaag aaaactaatc ctaatatagt tataattaaa
     9901 gagataatta tagatagtgt caaagcatta cctaatatct tctctgcttc ttctctattt
     9961 ctttccccta actttataga caatcttgtg ctagcaccta ctgataccaa cattccaaaa
    10021 gctattatta gagtaaatac tggcatagtt acaccaagac ctgcaatagc tatagagcca
    10081 ataccttcca tagaaccaat aaaagctcta tctactacat tataaagtga agttaccatc
    10141 attgctatta ttgcaggtat cgaataccta aataataaaa tccatatttt ttcatgtctt
    10201 aatgcttctt gattctccat gttgtcacca tttacaccat gtcataagtt atacaaattg
    10261 gtttatttgt tcttgggtct gttgctattt ctgcatcaat attaaataat tcttttagat
    10321 tatccttcgt cataacatca tatgcatcac cttcacaaac tattttacct ttcttaatac
    10381 ctatcatgtg gtcagcaaat cttgctgcat tattaagttc atgtattacc atgacaatag
    10441 ttcttccttg ttttttattt agttcatcta ataattttag tatctccaat tgatgtgcca
    10501 aatctaaata tgttgtaggc tcatccagta ataatatatc agtttcttgt gccaaagcca
    10561 tagctatcca tgccctttgt ctttgtcctc ctgataaatc ttccatattt ctatccttaa
    10621 attctgcaat tccagtaact tctaaactcc aatttacaat ctctctgtct tctttactag
    10681 aattaccaaa tccactttta tgaggaaatc ttccataaga aattaattct gaaacggtaa
    10741 gaccactagg agcttgaggg ctttgtggaa gtacagccat atcttttgct atttctttag
    10801 acttcttttt gtagatgtcc tctccattta tatatatttt cccactatta gggctaataa
    10861 ttctacctat agttttaagt attgtcgatt ttccacaacc attagcccct atgatggtag
    10921 ttatttttcc ttttggtata tttaaattta aattttttat aatatctgtg tttccatatg
    10981 atacatttag attcttagta tttatacagt tcataacaac accctttcct aatcatttac
    11041 caacattaaa taaataaagt acggcactcc tattatagaa actactatac ctactggtat
    11101 ttctataggt gctagaagat ttcttgatat agtatctgct attaaaagta ataatgttcc
    11161 tatcaatgct gatactggta ttaatctttt atgctttggt cctattattt ttctacctat
    11221 atgtggagct attaaaccta aaaatgaaat actacctgct actgctgtcg atacaccaga
    11281 taaaattact gcatatatta taagtcttct tctttgtttt tctacatcta caccaagact
    11341 tatagatact tcatcaccca aatttaaaac atctatatat ttagatttat aaatagttat
    11401 acccataaaa atcactataa atggaagcac agctattaca tatttccaac tggttcccca
    11461 aatgcttcct gatgtccatg ccataaccct attaaattcc tgtgttgtaa atttcaattg
    11521 gaatattgtc aaaattgaag taaaagctat gtttacacct ataccaataa gtagtaatct
    11581 tgatgaattt ataccacctt tccatgcaag agaatatata agaaatgctc caaataaagc
    11641 tcctataagt gcaacaatag gcattgtaaa tacagtcaag ttactcatac cttcatatac
    11701 attaccattc ataaggtata tataaactac tacaaacaat gcagcacctg aatttattcc
    11761 caaaattcca gaatcagcta aatcattctt tgtaactcct tgaagtatta cacctgctgt
    11821 tgataatgca gaaccaacca aaagtgctat tactattctt ggtaatctta atttaaatat
    11881 tgcaatttca tgattacgct gaccctgccc taaaaaagtc tttataacat ccattggttc
    11941 tatagaaaat gagcccatat ttaaactcaa taaaaaagtg ataaaaataa gcaccattaa
    12001 aattgatata acaagtaatg ttttagaatt tatttttata atatttttca tagtacctat
    12061 ttctccccct ttctaacaag gtatataaat attggcactc ctataagtgc tgttatagaa
    12121 cctactggtg tctcatatgg aggatttatc atcctactta atatatctgt atatacaagc
    12181 aatacagctc ctaatactaa agagctcggt attatatact tataatctga acctactatt
    12241 cctttagcta tttgaggaac tataagacct ataaaaataa tattaccaga cactgacaca
    12301 gatgctccag tcattaatat tacaagtact atacatataa atctaacaaa gttagttttt
    12361 tgtcctaatc ctattgccac ttcttctcca agacttaata tagttattct tggagccata
    12421 ataatagcta aaataaatcc aataccacca gctacaaata gcaattttat accttcccac
    12481 ttcactccta caagccctcc tgatatccag aaacttaatt gttgagatag gttaaaatac
    12541 atagatattg ctgatgccaa agaaattagc aatgtaccaa gagcgactcc tgcaagagct
    12601 aattttacat tattaactct tcctcttgat ttagaactta taaaatatat aaataatcca
    12661 cttatacttg ctccaataaa tgcaaacatc attacactaa aacttccatg tataagttga
    12721 ggaagttttt tttgaagtac cagtgatatc gaaattgcga atgtagctcc ttgtgtaatt
    12781 cctataacag atggttcagc tataggattt ctcgttattc cttgcattat tgccccagaa
    12841 actgctaaaa atcctccaac aagtgcagct gctattgccc ttggaagtct aacatccttt
    12901 actattctca tatttattcc atcttccatt tttatcaaac tatctataac agttgataga
    12961 ttcatatctt ttgccccaat agttatggat aatacaattc caaatacaag tacaactata
    13021 cttactaata tcaacaaata agttatattt tttttactca gcttagtcaa ttatcctacc
    13081 tccttttccc ttttaatcgt cagtataata tcatctatta agtattttaa ttttaagatt
    13141 tattaaaaat aggtgtgaaa aatgaatatc attatctcac acctactaag ttaagaatta
    13201 ctttgttaat tcatttttaa ctgattctaa taataactct tttccaattg gattgtatga
    13261 ttgactgaaa aatggagcag catcaagtat tgttacatta ccatctttta ctgctcttat
    13321 ttgagcccag atagcactat tttctaaatc ttttttatct gcttctgtag caattacaac
    13381 tatatggtct gcatctatat ctgttaaacc ttccatactt accattggta gtgttattcc
    13441 atcctgtttt ggcatattct taggtttagc taatttcata tcatcattga ttagagtacc
    13501 tattccacca tcactaaata ccataaattg acctgaactt gccaacactg gtaaataagt
    13561 tttttctcca ttcttttcta taacttcctt acctaacttt gtagcttttt catcatactt
    13621 ctgtagccaa cttttagctt cttcttcttt gtcaaataac tttgaaacat cagttaactt
    13681 gcttctccag tcatttgcat aatctttcat cataactaca ggagctattt cttttaattg
    13741 gtcatatatt ttttcttgtc tttgactcat tattattaaa tcaggattta cttctaatat
    13801 agcttccata tccatagtat ccatcattga gtgcccaact atctttacac ctttaagctc
    13861 ttctcttatg taacttggta atttatcagt ttcatatgag tctacatttg ctgttgcaac
    13921 aggcttataa cctgctaata atagttcttc actacttcct gatatatcaa ctattttttt
    13981 aggatttgat ggtatcttaa cctctccttt aacagattgt acaactcttg tctctgattt
    14041 atctttatcc tctgtattct tatctgaaca tgctgtaaca agtactaacg tagttataat
    14101 agatataaat attgcaagtg attttatttt tttcatgatt acttctccta cttgtttatt
    14161 atacttacat gatattgatt atcataagca catatttatt atagcatttt tctattttta
    14221 gtcaatatta ttttgtaaaa ggataaaaat tcctaattaa tcttaattat atatagctta
    14281 gataaggttt tcttatatta aacagtattt ttttaaagaa ttggacacaa atacaatgac
    14341 ttttcagtta aacaatgata aaatgcaatt tatagataat gaatttagta aggaggattc
    14401 aaaacatggt acatttagta tattgtgata atgcaggaaa aaaaggagaa aaggtcttag
    14461 ataaaatctt ggctggaaca aaaacaatgg ttattagagg tgctgctggt agaaaaatac
    14521 cacatagtag agtatttgaa gatgagttac tttattttat ggaaaaagga actgcaaaaa
    14581 tatctgcaaa agccactgtt aaaaaggtag aaaactatgt taaattaaca gaagatgaaa
    14641 tttctaaaat tttaaatgaa aatcaaccaa aattaaattt atctgaaaag caaaaagttc
    14701 gttggcataa aaaatgttta tgcttagttg aatttgaaaa tgttgaagaa ataacacctc
    14761 ttgattttga ccatcaagga aatatggatg attggcttat tgttgaaaaa attgaagatg
    14821 tagttgttgg tactagtata ccttacaatt atgagaagtc taaattttaa agtatcaata
    14881 acataatact atattgctaa actctactta aataaaaaaa gagtgcattt aataattata
    14941 aaaattattt ttatacactc ttttttaata ataattttaa attacttact ttctaatctt
    15001 tcatatacat ctataaattc ttctgctaat tgttgaaaat tcatagctgt cattaaatgg
    15061 tcttgtgcat gaataagtat aacacttatt tcagttttat taccattagc ttcattttgt
    15121 atcaattctg tttggaattt atgagcttta tgaattgatt ctttcgactt ttcaatacat
    15181 tctctagctt tatctatatt cccgctcttt gcttctttta ttgcttcaaa tgctagccct
    15241 ttcgcatcac ctgcaaatcc aattattcca aaagatatct ctatcatctt ctcatccatt
    15301 tattcgacct cctaaatatc tctatagctt gatatgacta atccattctt ttctaaaaat
    15361 tctttgactt ttttagatgt aagtatcttg tgctctttcg ttctatcaat agcataagat
    15421 gtagaattta aaatataatc atctaaaaaa gctgggtgag acataatatc cactacatca
    15481 tacttcatta tttcgtctat attttttata aaatattctt cacttacatt ttccttataa
    15541 aaactatcta ttaaaggaac tactttagaa tactctaaat tatattcaaa tccacctctt
    15601 attggaagat tatacttggt tacaatcttc tctattactg gctttaaact taataaagta
    15661 tggatatgat ggtgactatc taaatgagat atatctattc caagcccttt taccctatct
    15721 atttgagcac aaagttctct ataaatctca tcacaatcca ttttttcaat aatttcgtct
    15781 gtaatcctcc taataaaaaa accgtcttta tcaacaatag tttttaagtt agataataat
    15841 ggtctaccac aacttaaagt catatgaact ccacaattta aagttttatt ttctttaagt
    15901 aagcctactc catgttctac cccaggcata tttgccatca tagaagtaga cctaactata
    15961 ccattattat gagctgaaat tattccatag ttcacagctt cacaatatcc aaaatcatct
    16021 gcatttatta taattttagt cataccataa cctactttct atagacaact atctattaaa
    16081 tctaggaaga taatctttat gagcttccaa catttcgtct aatatagttt ttgctaagtc
    16141 atcagatgga atcaatgggt ttatacaaag tgccaaaaga gcagattcat aatcgccata
    16201 tacagctgct ttagctgcca atacttcaaa tgatttgatt tgacttacta atccatgcac
    16261 agaatctggt aaatatccta tacttattgg ttttggacca ttttttgtta ttacactact
    16321 tacttctacc gcttgttcat ctttaaaatc tcttatagaa cccttattta atgtattaac
    16381 tacttggata tcttttttgt cgttgtatat agaacttatt aaattacaag cagcatcact
    16441 atagtaagcc cctcctcttt tttcaagttg aggtggtttt acatctaatt tttcatcctt
    16501 atataattca aataattgtt cttccaattc tttaactact tgacctctag cttttccctc
    16561 tttaaactct ctcaactcat cctcaagcat ttctttagtt ttatagtagt atctatgata
    16621 tggacaaggt attgcaccca aagattttac aaattcagca ttaaaatcaa ttgcctttat
    16681 attttgcata cttatatctg ctttgacaaa tttatcaatt gcttcttttg taacatcttc
    16741 tccatctaaa gatacattta gaccataaac catatgatta agacctgcaa aatccatact
    16801 tattctgtca ctttctacat taaatagttt ggcaacatca ttttttaaat gaattggcac
    16861 gttgcataga cctatatatc ttctaaaatt agtatatttg aatacagcct ctgttattat
    16921 tcctgttgga tttgtaaaat tcaccatcca tgcatttgga cataattctt ccacatcttt
    16981 tattatatca aatataactg gtattgtgcg aagggcttta aataaacctc cagcaccatt
    17041 agtttcctgc cccataactc catgagataa aggaatagtt tcatctttta ttctcgcatc
    17101 taaaagacct actctaagtt gtgttgtcac aaagtctgca tctcttagag cttctcttct
    17161 atctaaagta agatttattt tcatatctac tccagctttt tttaccatcc tcttagctag
    17221 atttccaact atctctaatt tttcttttcc agcttctata tctacaagcc ataattcctt
    17281 tacaggtaac tcttcatatc tatttataaa accctctaca agctcaggag tataacttga
    17341 acctcctcca attgtaacta tttttagtcc ctttttcatc tcattatacc atccttacct
    17401 ataatttaat atactctaca aaaccccata catttattta aaactctata catttattta
    17461 ccgaaataaa actacctagt tgttaatttt caatttatta gcttccattt tttcagacgc
    17521 tatgacaaat ggaatatata ttaatattga taatattaaa ttaaatagtg ctaaagctcc
    17581 accagtccag tcacctgtag ccattgctcc acctaatata ggaggtgtta cccaaggtat
    17641 tttaggtaaa attactggtg ctactaatcc aaaatctatt gctaagtatg atacagcact
    17701 acatattact ggtgcaagta caaatggaat cataaatata ggatttaaaa ctattggaag
    17761 tccaaatatc aatggttctc ctatgttaaa tattgcaggt ggaccaccta atactatcat
    17821 ctctttacgt cttcttccag ctattaataa agctactatt aatcccaata ccattccaga
    17881 accacctaaa tatacaaatg catcaaagaa tgcacctgca agtacatggt atccttccgt
    17941 tgctcctgct tgagccaagg cagcattttc tccacctaat ttcataagta tagtttctgt
    18001 aaaaggaagt gctatatttg caccatgaag tccaacagtc caaagtatat gaattataaa
    18061 tgcaataagc attgtagaac ctagactatc agctacattt gatagtggtg ctcctaaata
    18121 agtatttaaa atatctgtta agaaacttcc attagaaagc atttttataa atattcctat
    18181 tacagtaaat atcattatag ttaacattcc tggtattaac tttgcaaatg atcttgaaac
    18241 tgctggtgga actccatctg gcatttttat agttaatttc tttaccttag ataatcttac
    18301 aaatatctca gttgataata atcctattaa tatagaagag aataaagctg ctgtacctaa
    18361 gtaggtttgt tgtatcattc cccatccttc aactactgta gttcctccct caggaactat
    18421 tttaccaatt tgtggagcca ttataaaaaa gatagataaa gctataagcc cagattgtaa
    18481 cccatcactc tcataagatt ttgctagaaa ataagcaaca cctattacta agaatactgc
    18541 cattgtacct attgctcccc accaaaggtc gcctccaaat gtggtccagt tttctccaaa
    18601 tatacttgcc atcaaatttt tgtatgcttc caatggtaag ttatttatca atgttcccaa
    18661 tgcacctacc atagttatag gtatcattac tataaatgca tctcttactg ctactaagtg
    18721 cctttgagct cctattttag cagctacagg cactatatat ttatccataa atgacatgaa
    18781 tttttccatt ttacatcctc cagtacaact ttattttgaa ctatttaact ctacagctct
    18841 atctaacaca gcttttccat tacatcttcc ataatccatc atatttataa cttctactgg
    18901 aatattatat ggttttgcca tttcagttgc ttttcctaat acatatctaa cttgaggccc
    18961 taaaagtaag acatcacaat tttctatttc attttttaag ttcacttctg atatagccca
    19021 tatttttgct tctataccgt tttcctttgc tgcatcctcc atttttgtaa ctaataaact
    19081 agttgacatt cctgctgaac aagctaacat tatttttatc attttttccc ctccaaatta
    19141 gattcattaa atatttttta agtcataagt tttttaaact atacatttaa aatatgtttt
    19201 tctattaatt ttttcttaat gtatatatct tatttttaaa tgtatataca tttaattctt
    19261 gatattatta tataccaaat gtatatacat ttcaagaaaa ctttttcatt ttatttaaat
    19321 tttattcgac aacagcattc aaagttaatg tatatccata gtgatatgtg tgtgagtact
    19381 caaaaatctc actattttgt aacttacaaa tttggcttat atataaaagt ggctctggtt
    19441 cttctaattt tagattatcc atacactctt cattcgatat tacagcttgt acttctctct
    19501 ctgacatggc tatcttgtat ccacacactt cttcaacaaa attataaaga gaattttcac
    19561 aatgagtctt atttaaattt ggaaataact taactggcat ataagtaatc atatatgaaa
    19621 ctcttacatc attagctaat cttactcttt ttatttccca aacattttct tctaaactta
    19681 tattcaactt acttgataaa ttttcatctg cctgaactat ttcaagagat accaatatat
    19741 tagatacttt atagccttta ctttccatct ctttagtaaa tccacttaca gacgatatat
    19801 tatcatatat attatttgca ataactatac tacctacacc tctttgtcta gttatatagc
    19861 cttctttaac tagattatta agtgcagacc ttacagtcat tctagttaca ttaaattctt
    19921 cactcagctg tttttcagaa gggattaaat ccccttcttt caaactgtct gaatttatta
    19981 actctctaac atgattttct ataactttat aaattggctc tttcatttaa ttcactcctt
    20041 attaaatctt ttatataata agataatttt atatacaaac aaagaaaatg tctatacatt
    20101 taagataatt taaattttat tcagaaatct tgtacaactt tccgagtttt aataaagata
    20161 taaatagact tatcattaat atacctttta atatacttga taaggttata ctccaccata
    20221 caccatttag acccaacatt tctggtctag atataaagta agccaatggt actcttgcac
    20281 cagtcagtat agtaattata atagaaggta tataggttct ccctaatcct ttgaaagctc
    20341 ctgttgttat tatttctaaa cacataaaaa attgagaata gcctaatatt ttaagataaa
    20401 ctgctccttt ttctattgcc tcactttcat ctataaaaat agaaaatata ctctctcctg
    20461 caaacactaa tactaaagta gttattatac caagtacaga agatacagtt atagctattt
    20521 tgcaaccttt atttattcta gaatacttct tggcaccata attttgtcca acaaaactgc
    20581 ctaatgcaac tgatagccct tcagccgtca tccaggaaat tgcttctatt tgtgagccta
    20641 ctttttgtac tgcaacagcc acaggccccc atgatgctac tattactcct aatagcattg
    20701 agaatactgt aaacatacca ctttgtattg ctataggtaa tcccaattta tacagaactt
    20761 tataatattt agtctctatt tctttaaata gtcttaattt aaaatattct tctttacttt
    20821 taactattat atatataaaa catgtagtaa ctactatctg agcaaataca gttgcaatag
    20881 cagctcctgc aacaccaagt tttggagcaa atccccatcc aaaaattaat atagggtcaa
    20941 gtataatatt agttattaat cctactgtat taatacgaaa aggagtttta ctatttccaa
    21001 gaccattgaa aattgcagta aatactggat taataaaata aaataccata cctagagcta
    21061 ctattatcaa atactgtcta gacatggtta taacttctaa gtctcctaat ctaaaaaaac
    21121 ctatcaattg tttattaagt actattaaag aaattgtata tattattgct agtataatat
    21181 ttatttctat tgctgacttc atatatgatt tagtagtgct tatattattt gcaccaatac
    21241 tttgtgctac ttttatctct ccacctactt ttgaaatcat aataaatgcc attgcaagcc
    21301 atggaaaaaa accagctgtt ccaactgcag ctactgcttt acttccggct ttaccaaccc
    21361 atatcatatc tatcatgtta taccccatct gtataaatga agtccccatt attggaagtg
    21421 atagcttaag taatttttct gtaattcttc cttctgttaa attaagtctg tctttcattt
    21481 tccacctctt ttatattaaa tttatttgct tatatagcta gattaatcat tttatttgat
    21541 tgagttcact atccttcaat ttatattatc atatctcagg attgcatctc aatcatactt
    21601 atgtttaaga tacagtattt tacctatcta tgataaaatt aatacttatg aatataaatc
    21661 tagccatttc atcaatacat attctaacct ttaacttaac ttcaattaag tatacatatc
    21721 gtaaccaatt agaatatttt atacggtatc ttccattaat aaacaaccaa tatcaatgat
    21781 ttatatataa ttttatttat acataaaata gtatactata ataataaaaa aactatattt
    21841 gaatgcttta agcatgttta ctaattgtaa tttccacaca aatttgataa tatatgaata
    21901 tattatggtg aaatttatag atatatatta attatgggga gattgtggga ggttcttttc
    21961 acaagacatt taagctatga aaataaataa aaaattcaat agagttgata tgcaggtttc
    22021 aatacttact gcaattattg taataatatc ttcctcatgt gtatattttt ttaattatac
    22081 actaacttat aagagcatga tatactcttt aagtgagagg tcaaaacata tctatgaata
    22141 tgtagaaaga aatattgata aaaatacctt tactgaaatt aattctaaag aagaccaatc
    22201 aaaaaagtct tataaaagtt ctaaaatcct tttagaatct gtaaaaaata ctactggagt
    22261 catgtatcta tatactgcta aaaaaacaaa agatggatca ctagtatatg ttgttgatgg
    22321 tttaaattca tctaggagtg attttagaaa tgctggtgat ttgatagaaa aagagatttg
    22381 gggagacctt aacaaagcat tagataatca tatattgtta cctaaaaaga ttaaatcaac
    22441 atcttgggga tacatattta tatcgtattt tcctattcac aatgatgttg gtaaggttgt
    22501 tggagtaata ggaattgaat ttgaagcaaa acatcaatac aatacattta aatttataag
    22561 tatagttaca ccaataatag cattattaac ttgtctaata tcagcattga ttgcagtgat
    22621 tctatttaaa agaatatcta atcctacata tactgatttt gctaatacag attatttaac
    22681 aggcctaaaa aatagaaatg cttttgaaat agatatgaat aacttaaaca attcaaatct
    22741 aaagaatcta atatctatca tatcaataga tttagacggt ttaaaaaaga ttaatgataa
    22801 atttggccat cagacaggag acttatatat taagcaagca agtaaaatta tacaatatgt
    22861 aataaataaa aaatatatag tttatcgtat tggaggtgat gaatacataa ttattctaaa
    22921 aaatttatct cacaaagaaa taaataatat tataaataat attaatttaa aaatagttga
    22981 agaaaactca actaatgaat taaaattatc aatgtctatt ggatatgcta tttttgatga
    23041 aaaagtagat gtttctttat ttgatacata tcgtcgtgca gattctcaaa tgtatgataa
    23101 caaaaggaaa attaataaac aatatcaata ataaatttaa aaagtctatt ctttaaataa
    23161 taaatcacaa gaatagactt ttttaaattt gtgtttttat aagataaatt atttaattaa
    23221 agtttgttct tttttctaaa cgattcttta taaattcata tattactttc actaatatat
    23281 ttgtgactaa tattaacaca gctagtgatg ctgcctgtgc agtactcccc ttatcatcta
    23341 gttgtacaat tgatattgca gctacttttg aacttgcagt atatataaaa actaatgctg
    23401 atattgtcac catggagttt aggaaaaagt acataaagtt ctctaaaact gctggcaaac
    23461 aaagtggtat agttacgttc ttaagaattg atgcgctgtt tattcccatt gatttagcta
    23521 ccatatccaa ttctttatct actttcttaa tagcagtagt agctgttata aatgctactg
    23581 aaaagaaatg aactatatta catataacca ttataagtaa tgttccatat aatccattaa
    23641 acaaattctt aatgtatatt gtatccataa aattaaactc taatttatta aaaaataata
    23701 cataagctat acctaaaact aatcctggca atgccatagg caatgtactt aaaaaatacc
    23761 caaatgattt taaatatgga gctttttctg ttttttcatt caaatatgca aataagaaaa
    23821 caaagctcgt ccctagaata gctgtcaata gtgacatttt tattgaattt ataaatggtt
    23881 taatcccatc aatactgttt ttcatagaaa aatgttccag tgtcagtctt aaatcatatg
    23941 gccaaactga tattaatgaa gataagatta cagttaacat tacagctatt ataccaaatg
    24001 caataattcc tacaacccct cctaaaatac tatctcttaa cttattagtt ttaaccttat
    24061 atggaattga ctttgaagtt atatatgaat tttgattttt ttcaattttt ttagttacta
    24121 taaacgatat tattgcaggt attaaaagta atattgatac agttgctccc attggcatat
    24181 tctgttgccc tataatttgc ttatatacat ctattgataa tacattaaac tctcctccta
    24241 tcacctttgg agctccaaaa tctgtaaaac ataatgtaaa cgatacgaat gcagaactta
    24301 ttaaagagta ttttatactt ggtattgtta tgcttataaa ttctcttatc ttgcttatcc
    24361 ccatagtatt actagcttca tatagtctat aatcggttga acttagagat atataaagta
    24421 tttgaaatga ttgaggaaat acatatataa tttgtgccaa tattattccc actgctccat
    24481 atattggaac atgcactcca agtaaattag ttaccaaacc ttgatttcca aataaatata
    24541 ctagacctat tgcatgcatc attgttggag caaacatagg aaacatagct aatgatttaa
    24601 aaaaaccttt aaatttaatc tttgttctag ttaatgcata tgcatatact aatgcaagtg
    24661 ttagtgatat tacagttgat ataactgata caaatactgt attttttaga gaatttaata
    24721 gagattcaga agccaagtat tcttgaaagt tttgtaatcc tacaaagact ccatctttat
    24781 ctaaaaaagc ctgtacaaat agatatatta taggtgctaa taaaaatatt actaaagaca
    24841 taactaatac tgtaagcata cattttttta tatctaaatt catttttgat ttaagactta
    24901 tacttttaga acttatagca ttattattca tacacttatg ctcctttaac ttttggatac
    24961 tttaaatagt atttttcgtc tatacttatt tttacaaggt caccttctac taaatttaaa
    25021 ttaacccact cttttataga agcatcactt attatgaaat tttcatttaa tttatctttt
    25081 atttcaactc tcaataaatt ccctctaaac tctatatttg atattattcc ttggtagttt
    25141 tcttttgtcg atttctctat ttgaacatat tctggtctta cagttaatat agaatctcca
    25201 ttatcaaaac agtttgtatc tcctataaat tgagctgtaa ataggttttg aggattttgg
    25261 tatatttctt ctggagtccc tatttgcatt atatctcctc catttagaat agctatttta
    25321 tctgccatag ttatagcttc ttcttggtca tgtgttacca ttattgtcgt tatattaagt
    25381 tctttttgta atcttttaat gtccattcta agcttgtgtc taacctttgc atctaatgcg
    25441 gacattggtt catctaaaag taaaaacttt ggttcaagag ctaatgctct tgctattgca
    25501 atcctctgtt gttgcccacc tgataatgct ttaggatatt tatgagcttc attagtcaat
    25561 ccaactgttt caagtacttc cttaacttta ttatctattt ttccttttga aacttttctc
    25621 tcttttagtg gaaaagctat attgttataa gcagtcatat tcggaaataa tgcatatgat
    25681 tgaaatacta taccaaaacc tctctttgat ggctctaaat ttgtaatatc tttatcctgg
    25741 agaattatag ttccgctatt tacatcttca agacctgcta ttattctaag taatgtagtt
    25801 ttaccacatc cacttggcta ttattagtta gtccaaataa ataaaatact tataacaatt
    25861 gaaatacgcg atatacactg aacctcccat gactaaagtc acagggttcc tgtttcatag
    25921 aatttcgcat aattaaatgt tagttcacaa tactattcta acttaccttg acctttaaat
    25981 actctgttcc tttttaggaa taaaatacta ttcataccta taaatttatt caattttcaa
    26041 acaatcttgt tgaacaataa ctaattctat tgtattattt tagttgtaga ataaaactaa
    26101 atcggcaaaa ctagagaaat ttagtgacgc aaagctatag ggactaagac ttatataaat
    26161 atcttatgag ttatgtcagc cagttgccaa agagatattg ttctttttgt ttttatgaaa
    26221 gttttttagg gggataataa tttatatgtt tagagataaa atggataaat gtacacatgt
    26281 attgactgct tatattggta gttcatatga ttattgtgat tttatagata cacagttaga
    26341 tgattttata ctagagtacg gagaaaaagt tgtggaatct tgcttgcatc aagtaatggt
    26401 attggtaagt aagtataatt agatagacat tgatttctag agaacccttt ttagttttat
    26461 ctacttttaa ataatgatat gttaaaaaat taattaaaat atctttgtaa gaaaggataa
    26521 taatataaat gtgctttaag acttatatac taaaaagagt tgtaaataac aaacaactag
    26581 aaataactcg aagttttgaa ttagataatc tcatttgtac tgcaacagag attttgaaag
    26641 atgatgagat tgatttaata attattgatg aaaaaaatag tattgtttgg agaaatgaaa
    26701 aaagcactcc ccagcaagag aatgcttagt tatagctata gaagtgtact acgaatacac
    26761 ttctattttt ttattatatc ataaattatt catagtatta agtaaaaata gagctaatac
    26821 aatcagtttt aaaatagctt gaatagcttt cttgagttat ttacctttgc ataaattatt
    26881 gaatatttga aataaaaaaa gataggttta cccctcatct tcaatcaaat taaagtctat
    26941 atttatagta tataattctt tacttctatc catttcaaat attgatattt ccatatcata
    27001 tgatttatta taggatttat catatatatt cttacattta atattaattg tacccgcctt
    27061 aatatttttc ctaatatata taccatcttc attattttct ctaaaattat ctataagttt
    27121 actagataat tccctaatat acatttcagt atatgcgttt aaacctatct caacttcttg
    27181 taaagaagaa atatattctt tctcgacttt aatattttcg ttttcatcaa attcatcaaa
    27241 gaaattatta catacttcta aatcatcatc tgttaaatat tcagttttaa ctatattcag
    27301 aggtttttcc caaaatactt ctatttgtat tgctgcttct ttccctacat tcacaagtgt
    27361 taattctata atatctgaac atcttggcac ttcgcttgat gtcttaattt cattaaacat
    27421 acaaggacta ccataagttt tatacaaata tgcatttttc ttaataggaa taaccttagg
    27481 ctctctttcc atagatatac tttcttttaa ttcttcatta tcttttttag ccatttctct
    27541 agttatcaat atagcaccta atgttgctaa ccctccaaat ataccaccta aaaaactgcc
    27601 ccaaaaactt aaccactcac catctgttga attatgtgtt tttatatccc aatataacac
    27661 cctatttatt aacgatggta agccaataca tataataaga attgctatta cacttataaa
    27721 tatggttagt atcttatgct tttcaatcca acttttattt ttattgttta tattgctcat
    27781 tactttccct ccgttctata atatatattt taccattttg tacaaaatgg taaaatatat
    27841 tatgggataa atattatttg gatataaact ttccttttgt attaagatat tatcaatctc
    27901 ttattttatc tataatatgt tatttttaag cctaaaaaag agataactat tttaatccta
    27961 gttacctctt tttatgttat tttggccata tagatatacc tattgtaata gccattgtag
    28021 caattcctat tatagtagtt atacataatg caataatcca tttattagtt gaatcaattc
    28081 tatcttctat accttttata ctattcttta tttcacttac atccttttct gtcacctttt
    28141 tatgttcgtt tatagcttct tttaaatcgc tttttaactc aattctttct ctagctaaat
    28201 cattttttag ttctattcta tctttgtcta aatcattctt gtattcttta aaaataccct
    28261 ttatgtcatt aaattgctta tctatacttt cttttacttc cttattattt tgatgtactc
    28321 ttttttcagt ctctaatatt cttttttcag tttctagtat tcttttttct tcttcattca
    28381 tattacctac ctcctctgaa gtagtagcta aaaaccaatt atctccaata catttatttt
    28441 tctccatcaa agtactattt aaagtattat aatagtctgt atatttgttg attttacttt
    28501 tatccttgtt ggaattaaac tttattactt tttctttttc tttattatat tcacccttca
    28561 taactattct tcctctatca tagactctaa atgttcaatt attgattgta tttctgattt
    28621 agatagttct aattccaaag attttttatc atttctaaat agtttcaaaa taaaatcatt
    28681 ttctctagcc aaagaagata tagaatataa gccaattgga ttgattctct gaatatatga
    28741 gttatctaat atatcatatg tactatttga aaaagtatac aaatcatttt taaagttttg
    28801 gcttatattt acattttcac cttcattata aaagtctttc agtttaattc catactcacc
    28861 tataaattct gggtcttctc ccaaaatttc atttgcttta gctttaaatt catgaataca
    28921 atgtggccaa gttggcatct gcatatataa attaagtaat agtattgtct tttcaaatat
    28981 tttgttatcc ttttcgtatt tttttataca gttaggaaaa tacgctacca aattagtccc
    29041 attttcataa gccatcttaa ctccccctaa aataatatta tttaatacta ttctacatta
    29101 taattacttt cattgcacta ttatttttta ttcctcctcc taccacttat tgatatatac
    29161 tcaactgctc ttcatatacc tgatttttta aagtttgtag atgacttatg gttatattta
    29221 atatatcatt ttttatttta taaattgttg tgatactgtc ataaaattgt agtcaaaatg
    29281 taaaatatat taactctatt gattttattt atttataaac tccagtgctt tataaagtgt
    29341 atcaaatcta tcattaccct ttatcatagt gtatctttct ttagtcatag aacttatctt
    29401 ttcacatgct ccaccaccaa cgacatataa attttctgtc tgacctggta cgtaatcttt
    29461 tatatcacat atcagtattt ttccatcatt atagccccaa ccaactacag ttgcagggat
    29521 tttgtcaact tctccatcat aaacgattgt atgtttgtac atgatttttc cctcaccatt
    29581 ctctttatta tctattgtct tatttaaaat accttctgct attaacttag caactatgtc
    29641 tttatgtcta atataataat ctgtatctgc tttactatct acgaaacata cttcaattaa
    29701 tattgctggt gcttttgtat gactaagcca ataaagaccc ctcacatctg attttgcacc
    29761 cctattttta aatacagttg ataattttga attaactctt tcggcatata ccttaccatt
    29821 gttagttttg tatattgtct ctgtacccat aggatttaga gttgttttat ttgcgttgaa
    29881 atggatttgt actgctaagt ctacattttg cctatttgca attttacatt gttctgctaa
    29941 gtagttatta gatttatcta cttttccagt atacacaata gctccacctt gtttcaacca
    30001 tttaacaatt aaatcagtta aaattctatt ttcttttcct tcgtctatat agccagttgc
    30061 tcctgttcct tttccactta aagtgtgtcc tggtactatt gctattttca ttatttatca
    30121 ccatccttca actgtttgta aacttgattt gcccctattg caactcccca acataaaatt
    30181 ccttgtaaaa ttgatgaagg attaaatcct aacatccata ttgaaaatcc aattcctatc
    30241 acaagcaata tgattggaat gtatttatta tctaactgtt tgtatttctt aaatccaaat
    30301 cctaacacat taagagcaac tactaataaa agcagttgct ctggtataaa acttattaaa
    30361 ttatccatat cttaacctcc taattaatta aaatattcct ttctgtatgg caaatataaa
    30421 gaaccctact aaagttgtaa ttataactcc tacaagccac ttgagtactc ctgtcagatt
    30481 ttttatatca ctacataggt tttgtatttg tatagcaaac tttgcttgct caatctcgat
    30541 tctatcaatt tgtttgccgt gttcttttac tcttgtttca agtgtactta ttttttcttt
    30601 cacaagttct tcattcatga aaacctccta ttgctttgta ttaaaaaaag aacattacct
    30661 attttgtaag ttctgctcct tctactacct cactatgttc tataatgtag ttttcaactg
    30721 ccatcctgta ctctgtgtta gtaacatcat caagttcaaa tactctattt tttagaggat
    30781 ttaatccctt atttaatatt ctctctgcta atattcttac tacaacatta tttatattca
    30841 ttataaaatt cctcctactt tctcattttc atttaataaa atttgatttt ctaactcttg
    30901 tattctcttt tcttcatctg aaataaatgt tggtatttcc tctaaaattg gttcttttgt
    30961 ttctatattt atacctataa ttctatttct tgtatagtct atacttccat acggaatatc
    31021 aacaaaatgt aattccgtta tttcatcatg ttctaatata tcccctgttg cttctccagt
    31081 ttggagtaat attttcccgg tttggtcaca tataattcta tttcctcttt ccatttaaat
    31141 taccaccttt catattattc ataagcatac catgtaaaac tttctccgac tttacaacgg
    31201 tatactacgc tactactatc agtgtattca tctcgataag tatataaaga aaattctttg
    31261 ctagtaatag tttttacaaa cataaaagca caaaaacgat attctccact actcttagga
    31321 acacgaccaa cagaagcaag ttgatttaaa ttattaatag cataaaaatt aacagtatac
    31381 ccatttccat aaaaacaaag tttttcaaca atgacaagac tagggatgaa atttaaatta
    31441 ataggaacag taagactctt agcataattt tcagcacttg aataccttga ataaaaattt
    31501 tcataatcac tttgagtaaa agtgtaagta cccttagccc atcttttacg ttggcttaac
    31561 tgattattta aattagtaat ctgattttga agttgttcag cagtagcatc tgaactatca
    31621 aaacctgttt ttattttctc tgataattca actaatgtat tatttaaatt tgcttctata
    31681 ttttttaatg ctaaagtgtt tataatactt gtttttccag ttttaaatcc tgcattaact
    31741 tcaattagtt tagaagatat gtcttgcaga ttcacatctt cgggcaatgg cattatattc
    31801 ttacttatac ttaacacttt ttctgctgta gcattattac tgtctgtaac aactatctta
    31861 agtgtgtgta gtgcattatc ttctagtgta tagttaattg ttttctctgt tgttaaatct
    31921 gttgttatag tttcttttaa cacatcatct ataaaatatt ctatttttgt aagcaatgta
    31981 gggtctgtgt ggtcagcttt aaatgtagct gtaatggaat tataagaaga tactgttaaa
    32041 aatggtaatg cttgtagtaa tgttatttt
//
LOCUS       DAYAPA010000036        31760 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.036, whole genome shotgun sequence.
ACCESSION   DAYAPA010000036 DAYAPA010000000
VERSION     DAYAPA010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 31760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31760
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.036"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            392..1591
                     /gene="hadA"
                     /locus_tag="ACGI59_001540"
     CDS             392..1591
                     /gene="hadA"
                     /locus_tag="ACGI59_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocaprenoyl-CoA:2-hydroxyisocaproate
                     CoA-transferase HadA"
                     /protein_id="HFG9739332.1"
                     /translation="MLLEGVKVVELSSFIAAPCCAKMLGDWGAEVIKIEPIEGDGIRV
                     MGGTFKSPASDDENPMFELENGNKKGVSINVKSKEGVEILHKLLSEADIFVTNVRVQA
                     LEKMGIAYDQIKDKYPGLIFSQILGYGEKGPLKDKPGFDYTAYFARGGVSQSVMEKGT
                     SPANTAAGFGDHYAGLALAAGSLAALHKKAQTGKGERVTVSLFHTAIYGMGTMITTAQ
                     YGNEMPLSRENPNSPLMTTYKCKDGRWIQLALIQYNKWLGKFCKVINREYILEDDRYN
                     NIDSMVNHVEDLVKIVGEAMLEKTLDEWSALLEEADLPFEKIQSCEDLLDDEQAWAND
                     FLFKKTYDSGNTGVLVNTPVMFRNEGIKEYTPAPKVGQHTVEVLKSLGYDEEKINNFK
                     DSKVVRY"
     gene            1630..2430
                     /gene="hadI"
                     /locus_tag="ACGI59_001541"
     CDS             1630..2430
                     /gene="hadI"
                     /locus_tag="ACGI59_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyisocaproyl-CoA dehydratase activator
                     HadI"
                     /protein_id="HFG9739333.1"
                     /translation="MYTMGLDIGSTASKGVILKNGEDIVASETISSGTGTTGPSRVLE
                     KLYGKTGLAREDIKKVVVTGYGRMNYSDADKQISELSCHARGVNFIIPETRTIIDIGG
                     QDAKVLKLDNNGRLLNFLMNDKCAAGTGRFLDVMAKIIEVDVSELGSISMNSQNEVSI
                     SSTCTVFAESEVISHLSENAKIEDIVAGIHTSVAKRVSSLVKRIGVQRNVVMVGGVAR
                     NSGIVRAMAREINTEIIVPDIPQLTGALGAALYAFDEAKESQKEVKNI"
     gene            2445..3671
                     /gene="hadB"
                     /locus_tag="ACGI59_001542"
     CDS             2445..3671
                     /gene="hadB"
                     /locus_tag="ACGI59_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(R)-2-hydroxyisocaproyl-CoA dehydratase subunit
                     HadB"
                     /protein_id="HFG9739334.1"
                     /translation="MSEKKEARVVINDLLAEQYANAFKAKEEGRPVGWSTSVFPQELA
                     EVFDLNVLYPENQAAGVAAKKGSLELCEIAESKGYSIDLCAYARTNFGLLENGGCEAL
                     DMPAPDFLLCCNNICNQVIKWYENISRELDIPLIMIDTTFNNEDEVTQSRIDYIKAQF
                     EEAIKQLEIISGKKFDPKKFEEVMKISAENGRLWKYSMSLPADSSPSPMNGFDLFTYM
                     AVIVCARGKKETTEAFKLLIEELEDNMKTGKSSFRGEEKYRIMMEGIPCWPYIGYKMK
                     TLAKFGVNMTGSVYPHAWALQYEVNDLDGMAVAYSTMFNNVNLDRMTKYRVDSLVEGK
                     CDGAFYHMNRSCKLMSLIQYEMQRRAAEETGLPYAGFDGDQADPRAFTNAQFETRIQG
                     LVEVMEERKKLNRGEI"
     gene            3671..4798
                     /gene="hadC"
                     /locus_tag="ACGI59_001543"
     CDS             3671..4798
                     /gene="hadC"
                     /locus_tag="ACGI59_001543"
                     /EC_number="4.2.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(R)-2-hydroxyisocaproyl-CoA dehydratase subunit
                     beta"
                     /protein_id="HFG9739335.1"
                     /translation="MEAILSKMKEVVENPNAAVKKYKSETGKKAIGCFPVYCPEEIIH
                     AAGMLPVGIWGGQTELDLAKQYFPAFACSIMQSCLEYGLKGAYDELSGVIIPGMCDTL
                     ICLGQNWKSAVPHIKYISLVHPQNRKLEAGVKYLISEYKGVKRELEEICGYEIEEAKI
                     HESIEVYNEHRKTMRDFVEVAYKHSNTIKPSIRSLVIKSGFFMRKEEHTELVKDLIAK
                     LNAMPEEVCSGKKVLLTGILADSKDILDILEDNNISVVADDLAQETRQFRTDVPAGDD
                     ALERLARQWSNIEGCSLAYDPKKKRGSLIVDEVKKKDIDGVIFCMMKFCDPEEYDYPL
                     VRKDIEDSGIPTLYVEIDQQTQNNEQARTRIQTFAEMMSLA"
     gene            4846..5979
                     /gene="acdB"
                     /locus_tag="ACGI59_001544"
     CDS             4846..5979
                     /gene="acdB"
                     /locus_tag="ACGI59_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative isocaproyl-CoA dehydrogenase AcdB"
                     /protein_id="HFG9739336.1"
                     /translation="MLYNKEQELLRKAVRDFVSKELDTLPAEMDKTGVMPKELIKKLA
                     DAKFISSNIPEEYGGGGAGYVSYAIVMEEIARRCASTATFVTAGSSLASLPILYNGTE
                     EQKQKYLKGIATGELIGAFGLTEPGAGSDAGGQQTTAELVGDHYILNGRKTFITNGPF
                     CDVAIVIAVTDRSKGLRGTSAFIVESKWDGFSTGAHEDKMGIRGTETSDLIFENVKVP
                     KENLLGKEGQGFKIAMGTLEVGRIGVAALALGIAQGALDEAVKYTKQRVQFGKPIAKF
                     QNTQFTIADMETKVCAARGLVYDAAQKRDAGMRVAQESAMAKYYASEIANEVAYKALQ
                     LHGGYGFIKDYEIERMYRDARIVSIYEGTSEVQKMVISSNVLK"
     gene            6012..6797
                     /gene="etfB"
                     /locus_tag="ACGI59_001545"
     CDS             6012..6797
                     /gene="etfB"
                     /locus_tag="ACGI59_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta"
                     /protein_id="HFG9739337.1"
                     /translation="MKILVCVKQVPDTNEVRINKETGTLIRDGVPSILNPDDANALEE
                     ALKIKDEHDDVTITVITMGPPQADFMLRECLAMGADDAILLSDRAFGGADTWATSNTI
                     AAGISKVGDYDIIFAGRQAIDGDTAQVGPQIAEKLDIPQVTYVQDFKIEGKDIIVQRQ
                     LEDGYELIKVSTPVLLTAVKELNTPRYMSVDKIVKAYKHDVKVWTIDDLDVNKEEVGL
                     KASPTKVFRSFTPEPRGKGEILEGKADEIASKIIIGLKQKHII"
     gene            6816..7853
                     /locus_tag="ACGI59_001546"
     CDS             6816..7853
                     /locus_tag="ACGI59_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFG9739338.1"
                     /translation="MNDIKDLSSYKNVWIFAEQREGKIAPVVIELLGEGRKLAKEVDA
                     ELCAILLGKDVDGLAKELITFGADKVYVADDALLEKYTTDAYTKVIKDAIDEIKPEIM
                     LFGATHIGRDLAPRIASRVGTGLTADCTKLEIDPEDKKIKQTRPAFGGNIMATIICPN
                     HRPQMSTVRPGVMDKAEKDETRTGEVIALDYKITQDDIRTTVLETVKTKKDLVSLTDA
                     NVIVSGGLGLGGPEGFEMLKKLADKLGGVVGSSRAAVDAGWIDHSHQVGQTGTTVKPN
                     LYIACGISGAIQHLAGMQSSDFIIAINKNPAAPILEIADYGVVGDLHEIVPMLIEKLD
                     SVDDLLEAIKA"
     gene            8336..9967
                     /locus_tag="ACGI59_001547"
     CDS             8336..9967
                     /locus_tag="ACGI59_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFG9739339.1"
                     /translation="MDNNLIGLIEHVENPAILCKESGEIIYCNHLIDSIFSFLDIKKP
                     RNINELDSNFDKTEILTDSKKKIAFRELRMTAHIYNMKDNNNENNIVYLFEKSLISDK
                     VIEDIIEHIDEVVVVFNKDGVIEKMNTVSDEILPFKRTEVLGRNITDLVRQGLVEEPI
                     ILNMLKVKKKIYRNIVYPDGKLIAYTAVPRWDSKGKLTGGVLTGRDISRVIKLESQIK
                     YSDISEDTEYISQSKIMDNIKKVVKRAAASDSSIFINGESGVGKEIIARTIYKYSSRR
                     DKPFIAINCGAIPNELLESEFFGYEEGSFTGAKKKGKKGLFEEANGGTIFLDEIGELP
                     MQMQKKLLRVIQENTITRIGGSKPIKIDVRYISATNISHEDLRNNLKFRQDLYYRLSV
                     IPVKIPPLRERKEDIVPLVNYFLKLYNEKYNREVEVSPKVIELLEEYSWPGNIRELKN
                     IIERFVVLSAKNVIGEDEFNMLINLDMIDNETDDLSPIVVNGIMNLNDAYKIVDQIMI
                     SKAINKYGSITKAAEVIGIYPSTIHRKIKSGHIHV"
     gene            complement(10102..11028)
                     /gene="fba"
                     /locus_tag="ACGI59_001548"
     CDS             complement(10102..11028)
                     /gene="fba"
                     /locus_tag="ACGI59_001548"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0030388 - fructose 1,6-bisphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HFG9739340.1"
                     /translation="MALITTKEMFKKAYEGGFAIGAFNISDLEQLQGVLKAAKAKNSY
                     VMIQASMSAVKYAGPHTLVEMVKAASDEIGVDVALHLDHGPNMDAIKTCIDAGFSSVM
                     IDGSHFDFEENVRITKEAVEYAHSKGVVVEAELGVLAGTEDDVTSDVHKYTQPAEAVE
                     FVERTGVDSLAIAIGTSHGAFKFKGEAKLRFDILEEIQSKLPGFPIVLHGASAVDQNA
                     VATCNEFGGNIAGAKGVPVDMLRKASSMAVCKINMDTDLRLAMTAAIRKFLAENPKEF
                     DPRKYIGAGRDAIQAVVESKIDDVLGSANSIN"
     gene            11358..11687
                     /locus_tag="ACGI59_001549"
     CDS             11358..11687
                     /locus_tag="ACGI59_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1904 domain-containing protein"
                     /protein_id="HFG9739341.1"
                     /translation="MPQIKIRGINENDICKISEKMINDLVEAVKCPRDYFEIECIKSV
                     AIRDGKIADVYPFVEVAWFDRGQEVQDIVARIITDSIRNNLDVESIDLAFTVFEKEKY
                     YENGEHF"
     gene            11687..12409
                     /locus_tag="ACGI59_001550"
     CDS             11687..12409
                     /locus_tag="ACGI59_001550"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFG9739342.1"
                     /translation="MAKKQRIDKILSNLGYGSRSEIKKYCKQGSVVVNGSEVSNPGTQ
                     VDTENDEILFNGEEVIYREYIYLMMNKPDGYISATTDKYDPTVLDLIDLSYLAFEPFP
                     VGRLDKDTEGLLVLTNDGKLSHRVLSPKKHVPKTYYAKIDGVVTEEDVEAFLEGVVLD
                     DGYKTMPSQLNILKSDDESEIELTIHEGKFHQVKRMFESVGKKVVYLKRLSMGNLKLD
                     ESLELGEYRELTDEEVKLIEER"
     gene            12436..13797
                     /gene="rlmD"
                     /locus_tag="ACGI59_001551"
     CDS             12436..13797
                     /gene="rlmD"
                     /locus_tag="ACGI59_001551"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFG9739343.1"
                     /translation="MSVKKKDVIEFEVDKMEFGGTSLSQVGDRVIHMKGGISGQKVRA
                     GVKKVRSKKAEVKMIELLENSPLETEETCKHFRECGGCTLLSVPYEKQLEIKEKQVMD
                     LFLKQDLFGFQFLGIEGSPENKYYRNKMEYTFGDEVKNGPLTLGLHKKGKHIDIQTVE
                     ECMLVDEDFSKILVSSVEFFNEKKLPYYRTMNHKGYLRHLVVRKGIHTNEIMVNIVTS
                     SQEDFDMYEFKDMLLGIDLKAELVGVLHTINDGLADAVQCDELRVLYGRDYIQEEILG
                     LKFKISPFSFFQTNTKGAEVLYSIARDFIGDYNDKVVFDLYSGTGSIGQVMAGAAKKV
                     YGIEIVEEAVVAANENAKLNGLTNCEFIAGDVAKVVKDLKDKPDLIIVDPPRPGIHKD
                     AIRDICGFGAKEIVYISCNPKSLVVDLVDFKGYGYEIKMVKCMDMFPNTPHCETVVLL
                     RRK"
     gene            14189..16258
                     /locus_tag="ACGI59_001552"
     CDS             14189..16258
                     /locus_tag="ACGI59_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFG9739344.1"
                     /translation="MQLTKIILSNYRCFGEEPTTIEINNLTGFIGHNSSGKTALLSAL
                     MKLFGEKSSDRIIERSDFHIPESIDPDSISKNELYIEAQFEFPELERGKDSISIPVFF
                     KNMVVSEPGGKPYLRIRLEANWEKSSNPEGSIDSNVYYITSTTEEINPEDKRNIKRYE
                     LDQIKMIYVPAIRRPDEQLKNVSGTIISRLLNGISWSEQTKARIKTKASETEEIFFEE
                     KGVESLKKSLETQWNSYHKDIRYSNPKINFNSTDLETILKKVEVTFSPTGTHRDYRVE
                     ELGDGLRSLFYLSLVNTFLEIEEEILKSINDGKEEDIFTLIPPVLTIVAVEEPENHIS
                     PHLLGRVVEKLNFISTKENSQTILTSHSPAIVKRIEPTDIRYFRICKEYECTKVKSII
                     LPNEEKEAEQFKFVKESVLAYPELYFAKLVVLGEGDSEEIIIKQMIELKAESVDSSEI
                     SIVPLGGRHVNHFWRLLNDLEIPFITLLDLDRERDGGGWGRIKYAIQQLINIGHDKNV
                     LLYTCEGISQDNEFKEMHTWDVTDTENMAKWIEMLKGYDVYFSAPLDIDFAMLQSFKD
                     KYISALTSAEGPRIKDHGKIQDIVINEDAEDESLKAAYKDRIDSDIKSTLKQEGGDGS
                     TYTEGEKELMIWYSYFFLGRGKPTTHLSLFSNNTDIDFESFPYCLKEFVNSIIEKMID
                     DSGGGRK"
     gene            16255..18120
                     /locus_tag="ACGI59_001553"
     CDS             16255..18120
                     /locus_tag="ACGI59_001553"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFG9739345.1"
                     /translation="MKRNWVSKEDWSPAAGFRLEVEAMNTVINDKNTLVVAGPGAGKT
                     ELLAQRACFLLETNTCRYPKKILAISFKKDAAENLKERLELRCGKELASRFESKTYDA
                     FAKEIVDRFRNSLDEAYRPLNNYNIAENRDVRRAFEIAGLNFRGNQTEFNKVYGNKLS
                     INKLPLHTDFVDKIFIDAWNVLLKGNKDTNFVSSLTFGMISRLAEYLIRTNRYIKKAI
                     EMTYSHVFLDEFQDTKSIQYDLVKACFFKTETIITAVGDGKQRIMLWAGARKSIFEDF
                     QNDFLSQKRTLIMNHRSAPRLVEIQKAMYNSLNEEAIDLKTNEKWNYEDGEVYLRLFE
                     DYIKEADILSEEIVELVETGIEINEICILVKQSVDIYSKEIINKLSERNIKARNEAMY
                     QEILKEDIIKTLVSLFRLSLSDRNPDDWDYIYDILGELYGIDEGYNPKILNDFITKVE
                     LMINDLKVNLNITDEKQFSDLVDSKVEYISYEKFASKFQKYKSKDYFDDLVDKFKKLV
                     WIEYNETKDWIKAIDNFEGKNSIPIMTIHKSKGLEYDTIFFVGLEDSAFWNFKNQPDE
                     DRCAFFVALSRAKRRIDFTFSLDRPAGFSNSQKHYCINEFYELFDNTEIVKKIEY"
     gene            18244..18438
                     /locus_tag="ACGI59_001554"
     CDS             18244..18438
                     /locus_tag="ACGI59_001554"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9739346.1"
                     /translation="MKKKTKISKKIVSFIMVFITIVTTILTSSVSAYASELDMSEQTG
                     FNYLGISPITGKKLIITFIV"
     gene            18441..19058
                     /locus_tag="ACGI59_001555"
     CDS             18441..19058
                     /locus_tag="ACGI59_001555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9739347.1"
                     /translation="MDNKIVFCVEPGIKTSSGGGYISEELGDKFISTTLPNYHKRKAE
                     ILEKVNRHNILPSWNNEKVAVKSGETIELEDKNGIIREMQLESNNTGTDILLEENTLK
                     ITANKNSNSGVISYIKIPKNEVGISIVYRKHNYQSLSEFHMESSMSANLNINVIKLGI
                     VKVKKIDEGTGKELANAKLRFEYDGDSKEIITGSNEIASIEDIIG"
     gene            19050..19571
                     /locus_tag="ACGI59_001556"
                     /pseudo
     CDS             19050..19571
                     /locus_tag="ACGI59_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901831.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
     gene            19684..19845
                     /locus_tag="ACGI59_001557"
     CDS             19684..19845
                     /locus_tag="ACGI59_001557"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9739348.1"
                     /translation="MAIAIIGVYLGFKREFSKLIGFLVVALIAVGLVFNTAGVKDILL
                     ELFNKIIGV"
     gene            20085..20312
                     /locus_tag="ACGI59_001558"
     CDS             20085..20312
                     /locus_tag="ACGI59_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFG9739349.1"
                     /translation="MDIVKVFANNVRKYRNKKGLSQEAFAEKAGLHRTYICAIERKKR
                     SIALDNVQKIADALEIETYLLFIEGEEKECL"
     gene            20309..23248
                     /locus_tag="ACGI59_001559"
     CDS             20309..23248
                     /locus_tag="ACGI59_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFG9739350.1"
                     /translation="MKKLPIEKLIENLGFSGSKNLVNIDMLTKLDISKHDVKTLKVIN
                     PYAIYLVNSKPFILFIESSFEEKKLQQISKLIWNAQIPIAFICDSNSVKIFNGKALDL
                     KSYLIEEIYNQSIEDFSEESEFSYLKISDPLFWDQYTKQYTKTHLNDYLLDNITSLTN
                     KLKKKYKVKFATKLVLRLIFIRYLIDRGVDIAYKNFNHDIKASQKELLKITKNKVELY
                     ELFKYLKEKFNGNLFELNDEINNTCLTDEVFILLSDFLSGKISIDNGQMSLFSMYDFN
                     IIPVELISNIYEILLGKEIRNKDNAFYTPNYLAEYILDKTTLEFLKNNRQYKILDPSC
                     GSGVFLVNSFRRIIDMNLGDKLFCEDDKLLKELLLSNIYGIDINEDAIDVTIFSLYLT
                     ILDYKDPKILSEFKLPNLKGCNLIASDFFDDNKLESLKKINFDFIIGNPPWGSVSDGL
                     HLDYCNKNGYNDMQQNKEISRSFIFRAKDFSSRNTVCSFVVHSKILYTQKKPSKKFRE
                     FLLKKSQIFSVVEMSSVRKLVFKNADAPAAVISFKYNESEENLNNRIIYTSLKPNVFF
                     KLFNIIVIEKYDVKYISQKLLLENDWAWKTIVYGFSKDIDIIMNIKKKYQTVNEIILK
                     NSFVHGTGIQINGNESHDATHLKGKWYVDSKKGIDSFYVDINKGKELTQDKMRRPKAN
                     QQEIFIPPYVLLKKGVDTKTYRYRAAFSNDAFVYSDAVTGICGKKDNLTTLYCLTGML
                     NSSFYSYLNIMLGTSIGIEREQGFSTEIFTYPAIESDEIANLVVKIQEATKLEKTTMF
                     LNEKNSCKYIKQLDKLILKSFNLNDDKFIDYAINIQIPELTNSKSTNIYRKITKEDLI
                     NYSECFKEQFSSIYRRVGKSISISVYPKILNRFTVFELEVTDEKTDREIMVKAEADNN
                     MELFTKIIRYDYNDVFHQIRDIIHFGENSFYIIKPNCYKYWHPAIAEMDLADVLDQIL
                     SDTGGEE"
     gene            23248..23922
                     /locus_tag="ACGI59_001560"
     CDS             23248..23922
                     /locus_tag="ACGI59_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9739351.1"
                     /translation="MGGFKNSSLNFYLKQGLYEIIIDGLIDCCLLMSKSCIEQSRKIL
                     NHEEKIRTHLVENYLENDIERRKTSLSKQHLRFEVEAQENYDNKNESYEGRVDIKVIS
                     KDTLADRRAYYIIECKRIDGKETLNKKYITEGVQRFITEKYSSHYGKNIMLGFIVKDI
                     NIQSNIQKINSLNQNILYTYIDTDMLTVRSDSENNYAVCESKYKVKSKLLLLNHIFYN
                     FSSIIR"
     gene            24083..24499
                     /locus_tag="ACGI59_001561"
                     /pseudo
     CDS             24083..24499
                     /locus_tag="ACGI59_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003455547.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            24459..26906
                     /locus_tag="ACGI59_001562"
                     /pseudo
     CDS             24459..26906
                     /locus_tag="ACGI59_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            27046..27468
                     /locus_tag="ACGI59_001563"
     CDS             27046..27468
                     /locus_tag="ACGI59_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9739352.1"
                     /translation="MRLEDIIDKESYEEKYDDLTVEIKKLSKEKKTLKASLINEKSIK
                     ARLREFRKILERNELLEKFDRYVFASIVDKVIVGVINEDGIVDPYKITFVYKTSFDDK
                     QDGHKFINKSPLETYSKDNNDNKKKCSYTNNETLRDCS"
     gene            28006..29040
                     /locus_tag="ACGI59_001564"
     CDS             28006..29040
                     /locus_tag="ACGI59_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHYH domain-containing protein"
                     /protein_id="HFG9739353.1"
                     /translation="MFKKKLIAVAMSITMISVGSFSYAHSGRTDSSGGHRDNKNKSGL
                     GSYHYHCGGYPAHLHNNGGCPYTGGGSSSGTTTSVNNEEKQKRSVGEKGYNQGYEDGY
                     KGNYSSSNYSGDYSDTYESKYSEGYEKGKAKLEEEEKVAKETGYNLGLTGAKSNNTYE
                     KEVLKNAYDTGYSTGYNEYKTKKIEEYKAKGIEDSNKDKEKMTFEENIDSEFIDAYNN
                     AYDEIQEQLKNDYTTQGFESAIKGERFDTSTIGNVKYANWFKEGYDNGKVKLPKAKES
                     VYNQGYNEEDFSIPDEMKSIETKLKGVYDEGLEKREEEKSRNATYGVGAGTAAVVAGV
                     VYKKKKRKKI"
     gene            29127..29330
                     /locus_tag="ACGI59_001565"
     CDS             29127..29330
                     /locus_tag="ACGI59_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4236 domain-containing protein"
                     /protein_id="HFG9739354.1"
                     /translation="MGFNFRKSINLGGGLKLNLGKKSVGISAGVKGARVSVNSKGRKS
                     ATLSIPGTGISYTKTSTKKRKPK"
     gene            29501..30661
                     /locus_tag="ACGI59_001566"
     CDS             29501..30661
                     /locus_tag="ACGI59_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp27"
                     /protein_id="HFG9739355.1"
                     /translation="MKLSKILLAIGMSVSLLIASCPTSNALSSINSIKGTNKYETAGL
                     IADKQNYDIAILINSENGLSDGLSASGLSGAKNAPILLTKKDTIPSETLKRLEKVKEV
                     YLIGGKNSIGASVQKILSDKKISINRISGTDRIDTSHKVAKEIGRVSTVKKVILTNAF
                     KGEPDAMSVASIAVRDKSPIVLTDGNTTSFATDGLETYAIGGKVSMSDKLINETKATR
                     IGGVDRYDTNKQIINKFYNGSKEFYIASGTDLVYSLVGSTIAQKTPIVLVGNGSNKSI
                     LKNASKITAIGNIDDVVIKQCLNITNNVGDSNTGIVKDTNSNNTNKPSNEPSSNNPGL
                     TDKSTVYVTPNGKSYHLTKNCSTLKRSKTINKVTLKQAKSQGKIDPCNVCVK"
     gene            30920..31060
                     /locus_tag="ACGI59_001567"
     CDS             30920..31060
                     /locus_tag="ACGI59_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9739356.1"
                     /translation="MENLMMSIITGVIASYIYDKLKSHSERPTKSGWELNIKFQKSKS
                     SK"
BASE COUNT        12777 a         3346 c         5750 g         9887 t
ORIGIN      
        1 gtgtttttga gtttagcaat gaaaactttt tttgaaatgt caagcttttt cataaaaaaa
       61 tatataaaaa aaacttttat aagcatagaa agaatagcaa aatgcaatgg atagtggcaa
      121 gatgccacga tatgaaaaag attattcaat tgcatattgt taaaaaacat tcaaatcaag
      181 cgatattttg attaaatata aagttggcac gataaatgct taacaatata ggtgtaagac
      241 aataaaacaa taaaaaataa ttttaataat ttttaagtta ttttagataa gattaggaac
      301 tttgacatca gacagttaat atcagaaaaa agtattttat gagtgattta atcgaaattt
      361 tataattgat actattaagg aggaaaacgt tatgctttta gaaggagtta aagtagtaga
      421 actttcaagt ttcatcgcag caccatgttg tgcaaaaatg ttaggtgact ggggtgcaga
      481 ggttattaag attgaaccta tagaaggtga tggaataaga gttatgggtg gaacatttaa
      541 atctccagca tcagatgatg aaaaccctat gtttgaatta gaaaatggaa ataaaaaggg
      601 tgtaagtatt aatgtaaaat caaaagaagg agtagaaata ttacataaat tattatcaga
      661 agcagacata tttgtaacta atgttagagt tcaagcatta gaaaaaatgg gtatagctta
      721 tgaccaaata aaagataagt atccaggatt aatattctct caaatattag gatatggtga
      781 aaaaggacct ttaaaagata aaccaggatt tgactatact gcatacttcg caagaggagg
      841 agttagccaa tctgttatgg aaaaaggaac atctccagca aatacagcag caggatttgg
      901 tgaccactat gcaggtctag cactagcagc aggaagttta gcagcattac ataaaaaagc
      961 tcaaactggt aaaggtgaga gagtaacagt aagtcttttc catacagcta tatatggaat
     1021 gggaacaatg ataacaacag cacaatacgg aaatgaaatg cctttatcaa gagaaaatcc
     1081 aaacagccca ttaatgacta catataaatg taaagatgga agatggattc aattagcttt
     1141 aatacaatac aacaagtggt taggcaaatt ctgtaaggtt ataaatagag aatatatatt
     1201 agaagatgat agatataata acatagattc aatggttaat catgttgaag atttagttaa
     1261 gatagttgga gaagctatgt tagaaaaaac attagacgag tggtcagctt tattagaaga
     1321 agcagactta ccatttgaaa aaattcaaag ctgtgaagat ttattagatg acgaacaagc
     1381 ttgggcaaat gacttcttat ttaagaaaac atacgatagc ggaaatacag gtgtcttagt
     1441 taatactcca gttatgttta gaaatgaagg aattaaagaa tatacaccag caccaaaagt
     1501 aggtcaacat actgtagaag tattaaaatc tttaggctac gatgaagaga aaataaataa
     1561 ctttaaagat agtaaagttg taagatatta attagtaatt ctagattttt taaaaggaga
     1621 atgctaaaga tgtacacaat gggattagat ataggttcaa ctgcatcaaa gggagtaatc
     1681 ttaaagaatg gggaagatat tgtagcttct gaaacaatat cctctggtac tgggactact
     1741 ggaccatcaa gagttttaga aaaattatat ggcaagacag gtcttgcaag agaagatatt
     1801 aaaaaagttg tagttacagg atatggaaga atgaactatt cagatgctga taagcaaata
     1861 agtgaattaa gctgtcatgc tagaggggta aatttcataa ttccagagac aagaaccatt
     1921 attgacatag gtggtcaaga tgcaaaggta ttaaaattag ataataatgg aagactatta
     1981 aactttctta tgaatgacaa atgtgctgca ggtacaggaa gatttttaga tgtaatggca
     2041 aaaataatag aggttgatgt atctgaactc ggaagtatat ctatgaattc tcaaaatgaa
     2101 gtatcaataa gcagtacatg tacagtattt gcagagtctg aggttatatc acatttatct
     2161 gaaaatgcaa aaattgaaga tatagtggca ggtattcata cttcagtagc aaagagagtt
     2221 tctagcctag taaaaagaat aggagtacaa agaaatgtag ttatggttgg tggagttgct
     2281 agaaatagtg gtattgtaag agctatggca agagaaatca acacagaaat tattgtacct
     2341 gatatacctc aattaactgg tgctttagga gcagcgttat atgcttttga tgaagcaaaa
     2401 gaatcacaaa aagaagtgaa aaatatataa aaagagggat gaaaatgtct gaaaaaaaag
     2461 aagctagagt agtaattaat gatttattag ctgaacaata tgcaaatgca tttaaagcta
     2521 aagaagaagg aagacctgta ggttggtcaa catcagtatt tcctcaagag ttagcagaag
     2581 tatttgactt aaacgtatta tatccagaaa accaagcagc tggagtagca gctaaaaaag
     2641 gttctttaga attatgtgaa atagctgaat ctaaaggata ttctattgac ctatgtgcat
     2701 atgcaagaac aaattttggt cttttagaaa atggtggatg tgaagctttg gatatgccag
     2761 ctccagattt cctactttgc tgtaacaata tatgtaacca agttataaaa tggtatgaaa
     2821 atatttcaag agaattagat atacctttaa taatgattga tacaactttc aataatgaag
     2881 acgaagttac tcaatcaaga atagattata ttaaagctca atttgaagaa gctataaaac
     2941 aactagaaat tatatcagga aagaaatttg accctaagaa gtttgaagaa gtaatgaaaa
     3001 tatcagctga aaacggaaga ctatggaagt attctatgag tttaccagca gattcttctc
     3061 cttctccaat gaatggattt gacttattta cttacatggc tgtaatagtt tgtgctagag
     3121 gtaaaaaaga aactacagaa gcatttaagt tacttataga agaattagag gacaacatga
     3181 aaactggtaa atcttctttc agaggggaag aaaaatacag aataatgatg gaaggtatac
     3241 cttgttggcc atatatagga tacaagatga aaacattagc taaatttgga gttaacatga
     3301 caggtagtgt ttacccacat gcttgggcat tacaatatga agttaatgat ttagatggaa
     3361 tggcagtagc atatagtact atgtttaaca atgtaaacct agaccgtatg acaaaatata
     3421 gagttgattc tttagtagag ggtaaatgtg atggagcatt ctatcatatg aacagaagct
     3481 gtaaacttat gagtttaata caatatgaaa tgcaaagaag agcagctgaa gaaactggat
     3541 taccatatgc tggatttgat ggtgaccaag cagaccctag agctttcact aatgctcaat
     3601 ttgaaacaag aattcaaggt ttagttgaag taatggaaga aagaaaaaaa cttaatagag
     3661 gtgagatata atggaagcta ttttatctaa aatgaaagaa gtagttgaaa atccaaatgc
     3721 ggctgtaaaa aaatataaaa gtgaaactgg taaaaaagct ataggttgtt tcccagttta
     3781 ttgcccagaa gaaattatac atgcagctgg aatgcttcca gttggtatat ggggagggca
     3841 aacagaatta gatttagcta aacaatattt ccctgcattt gcatgttcaa taatgcaatc
     3901 atgtttagaa tatggattaa aaggtgctta tgatgaatta tctggagtta ttataccagg
     3961 tatgtgtgat acactaattt gtttaggaca aaactggaaa tcagcagtac ctcatataaa
     4021 atatatatca ttagtacacc cacaaaatag aaaacttgaa gctggtgtaa aatacttaat
     4081 cagtgagtac aaaggcgtaa aaagagaact tgaagaaatt tgtggatatg aaatagaaga
     4141 agcaaaaatt catgaaagta tagaagttta caatgaacat agaaaaacta tgagagactt
     4201 tgttgaagta gcttataaac attctaatac tataaaacca tcaataagaa gcttagtaat
     4261 taagagtggg ttctttatga gaaaagaaga acatactgag ctagtgaaag atttaatagc
     4321 aaaattaaat gctatgccag aagaagtctg ttctggaaag aaagttttat taacaggtat
     4381 attagctgat tctaaagata tattagacat tttagaagac aacaatatat cagttgtagc
     4441 tgacgactta gcacaagaaa caagacaatt cagaacagat gtaccagcag gtgatgatgc
     4501 gttagaaaga ttagcaagac aatggtcaaa catagaagga tgttcattag cttatgaccc
     4561 taagaaaaaa cgtgggtcac ttatagtaga tgaagttaaa aagaaagata tagatggtgt
     4621 tatcttctgt atgatgaaat tctgtgaccc agaagaatac gattatcctt tagttagaaa
     4681 agatatagaa gatagtggaa tacctacttt atatgttgaa atcgaccaac aaactcagaa
     4741 taatgaacaa gccagaactc gtattcaaac ttttgctgag atgatgagtt tagcgtaagt
     4801 tcattaaaat aaatttacta taaaatatga aaattggagg aagctatgtt atataacaaa
     4861 gaacaagaac ttttaagaaa agcagtaaga gattttgtta gtaaagaatt agatacttta
     4921 ccagcagaaa tggataaaac aggtgtaatg cctaaggaat taatcaaaaa attagctgat
     4981 gcaaaattta taagcagtaa tatcccagaa gaatatggtg gtgggggagc aggatatgtt
     5041 tcttacgcta tagtaatgga agaaatagct agaagatgtg cttcaacagc tacttttgtt
     5101 acagctggtt cttctcttgc ttcattacca atactttaca atggtactga agagcaaaaa
     5161 caaaaatact taaaaggtat agcaacaggt gaattaatag gagcatttgg tttaactgag
     5221 ccaggagctg gttctgatgc aggtggacaa caaacaacag cagaattagt tggagaccac
     5281 tatatattaa atggtagaaa aactttcata actaatggac cattctgtga tgtagctata
     5341 gtaatagcag ttacagatag aagtaaagga cttagaggaa catcagcgtt tatagtagaa
     5401 agtaaatggg atggtttctc aacaggagct catgaagata agatgggtat aagaggaact
     5461 gaaacttctg acttaatatt tgaaaacgtt aaagttccaa aagaaaactt acttggaaaa
     5521 gaaggtcaag gatttaagat agcaatgggt actctagaag ttggtagaat aggtgttgct
     5581 gccttagctc taggaatagc tcaaggtgct ttagatgaag ctgttaaata tacaaaacaa
     5641 agagttcaat ttggtaagcc tatagctaaa ttccaaaata ctcaatttac tatagctgac
     5701 atggaaacaa aagtttgtgc agctagagga ttagtttatg atgcagcaca aaagagagat
     5761 gcaggaatga gagttgctca agaatctgct atggctaaat actatgcatc agaaattgca
     5821 aatgaagttg cttataaagc attacaactt cacggtggat atggatttat aaaagattat
     5881 gaaatcgaaa gaatgtacag agatgctaga atcgtatcaa tatacgaagg aacatcagaa
     5941 gttcaaaaaa tggtaatttc atcaaacgta ttaaaataat aaataatcta atgaaattgg
     6001 aggtatagac tttgaaaata ttagtttgcg taaaacaagt tccagatact aacgaagtaa
     6061 gaataaataa agaaacaggt actcttataa gagatggagt tccaagtata ttaaacccag
     6121 atgatgcaaa tgcattagag gaagctttaa aaattaaaga tgagcatgat gatgtaacta
     6181 taacagttat aacaatggga cctcctcaag cagactttat gctaagagag tgtttagcta
     6241 tgggtgctga tgatgcaata ttattaagtg atagagcatt tggaggagct gatacttggg
     6301 ctacatcaaa tactattgca gcgggaatat ctaaagtagg tgactatgac ataatatttg
     6361 caggaagaca agcaatagat ggagatactg cacaagttgg accacaaatt gcagagaaat
     6421 tagacattcc tcaagttaca tatgtacaag atttcaaaat agaaggaaaa gatataatag
     6481 ttcaaagaca attagaagat ggatacgaat taataaaagt aagtacacct gtacttttaa
     6541 cagcagtaaa agagttaaat acacctagat acatgtctgt tgataaaata gtaaaagcat
     6601 ataaacatga tgtaaaagtt tggacaattg atgatttaga tgtaaataaa gaagaagttg
     6661 gtttaaaagc atcaccaact aaagtattta gatcatttac tcctgaacca agaggaaaag
     6721 gagaaatctt agaaggtaaa gctgatgaaa tagctagtaa aataataatc ggtctaaaac
     6781 aaaagcacat tatataagtt ggaggagtac aaactatgaa tgatataaaa gatttaagtt
     6841 cttataaaaa cgtatggata tttgcagaac aaagagaagg aaaaatagct ccagtagtta
     6901 tagaattatt aggagaagga agaaaattag ctaaagaagt agatgcagaa ctttgtgcaa
     6961 tattattagg aaaagatgtt gatggattag ctaaagaatt aatcactttt ggagctgaca
     7021 aagtttatgt tgcagatgat gctcttttag aaaaatatac aactgatgca tatacaaaag
     7081 taataaaaga tgcaatagat gaaataaaac cagaaataat gcttttcgga gcaactcata
     7141 taggtagaga cttagcacct agaatagctt caagagttgg aactggatta acagctgact
     7201 gtactaagtt agaaatagac ccagaagata agaaaataaa acaaactcgt ccagcatttg
     7261 gtggaaacat aatggctaca atcatttgtc caaaccatag acctcaaatg tctacagtta
     7321 gacctggtgt tatggataaa gctgaaaaag acgaaacaag aactggtgaa gttatagcat
     7381 tagactacaa aataactcaa gatgatataa gaactactgt tttagaaaca gttaaaacta
     7441 agaaagattt agtatctctt acagatgcaa atgttatagt atcaggtggt ctaggattag
     7501 gtggaccaga aggatttgaa atgcttaaga aattagctga caaattaggt ggagtagttg
     7561 gttcttctcg tgcggctgtt gatgctggat ggatagacca ttctcaccaa gtaggtcaaa
     7621 caggaactac tgttaaacca aacctatata tagcttgtgg tatatcagga gcaatacaac
     7681 atttagcagg tatgcaatca tcagatttca taattgctat aaacaaaaac ccagcagctc
     7741 caattttaga aatcgctgac tatggagtag ttggagactt acatgaaata gttccaatgc
     7801 ttatagaaaa attagatagc gttgatgatt tattagaagc tataaaagct taatagatac
     7861 aaactatata aatattactg attgaataag tcttattagt tagtaacata attattaacc
     7921 ctttgataag ttattgctag tgtgtatttg tcagttatta aaaagtctgt ttgaataaaa
     7981 ttttataata caagataatc tggataatcc ttatatgtat attgcttaca aattgtgagt
     8041 tatattctgt aaggattatc tttgtttttt taaataaatc ttaaatttaa ttaaattgta
     8101 ttaaatagac aaataattac taaatatgtg ataaaataca attaaaagca aaatgaaatt
     8161 tttaacaaac tataatcatt aaaattatat cttagttacg ttttaatgaa ataaactact
     8221 tgaaaagacg aatattataa ggagttgatt atatgtgctg atggtattta ttgtaagcaa
     8281 acaataaatt ttgtatgtag ttcaataaat tacttaatga aaggtgcgaa tattaatgga
     8341 taataattta atagggctta tagaacatgt agaaaatcca gccattttat gtaaagaatc
     8401 aggcgaaata atatactgta atcatctaat agatagtatt tttagttttc tagatataaa
     8461 aaagcctaga aatataaatg aattagattc aaattttgac aaaacagaaa tactgacaga
     8521 tagtaaaaag aaaatagctt tcagagagtt aaggatgact gcacatatat acaatatgaa
     8581 agataataat aatgaaaata atatagttta tttatttgag aagtctctaa tatctgataa
     8641 agtaattgaa gatataatag agcatataga tgaagttgtt gttgtgttta acaaagatgg
     8701 ggtaattgaa aagatgaaca ctgtcagtga tgagatatta ccttttaaaa gaactgaggt
     8761 acttggtaga aatataacag accttgtgag acaaggatta gttgaagaac caataatatt
     8821 aaacatgctt aaagtgaaga aaaagattta tagaaatatc gtttacccag atgggaagct
     8881 tattgcatat acggcagttc caagatggga ttctaaggga aagcttacag gaggagtact
     8941 aacagggaga gatatttcaa gggttattaa gcttgaatct cagataaaat atagtgatat
     9001 atcagaagac acagagtata taagtcaaag taagattatg gacaatataa aaaaagttgt
     9061 taagagagct gcagcctcag attcttctat atttataaat ggagaatcag gtgtaggaaa
     9121 agaaataata gcaagaacaa tatataagta tagttcaagg agagacaaac cttttatagc
     9181 tataaactgt ggagctatac caaatgaatt attagagtca gagttttttg gatatgaaga
     9241 aggttctttt actggagcta agaaaaaagg taaaaaggga ctttttgaag aggcgaatgg
     9301 tggaactatt ttcttggatg agataggtga gttaccaatg cagatgcaaa aaaaattact
     9361 tagggttata caagaaaata ctattactag aataggtggt agtaagccta taaaaataga
     9421 tgttagatat ataagtgcta ctaatatttc tcatgaagac cttagaaata accttaaatt
     9481 tagacaagat ttgtactata gattaagtgt tattccagta aaaatacctc ctcttagaga
     9541 aagaaaagag gatatagtac cattagtaaa ttatttctta aaactttaca atgaaaagta
     9601 caatagagaa gtagaagttt cgcctaaagt tattgagtta ttagaagaat attcttggcc
     9661 aggaaacata agagaattaa aaaacatcat tgaaagattt gtagtattat cagctaaaaa
     9721 tgtaattgga gaagatgaat ttaacatgct gataaattta gatatgattg ataatgaaac
     9781 agatgattta tcaccaatag tggtaaatgg aattatgaat cttaatgatg catataaaat
     9841 agtggaccaa ataatgataa gtaaggctat aaataagtat ggttcaataa caaaggctgc
     9901 tgaagttata ggtatatatc cttctaccat ccatagaaag ataaagagtg gacatattca
     9961 cgtttagctg tcagtttaaa aagacaatat aattaaaata ttataagata cataatttat
    10021 acaacttaaa agagatggct taaaaataaa tttaattact ttaagtcatc tcttttcttt
    10081 tataaatatt taaaagttat attagtttat agagttagca gaacctaaaa catcatctat
    10141 cttgctttca actacagctt gtatagcatc tctaccagca ccaatgtatt ttcttgggtc
    10201 aaattctttt ggattttcag ctaagaattt tctgatagca gcagtcatag ctagtcttaa
    10261 gtcagtatcc atgtttattt tacaaacagc catagaagat gcttttctta acatatctac
    10321 aggtacacct ttagcaccag ctatatttcc accaaattca ttacatgtag caacagcgtt
    10381 ttggtcaaca gcagatgcac catgaagaac tattgggaaa cctggtaatt tgctttggat
    10441 ttcttctagt atatcgaatc ttaatttagc ttctcctttg aatttgaaag caccatgaga
    10501 agttccaata gctattgcta atgaatcaac acctgttctt tcaacgaact caactgcctc
    10561 agctggttga gtatacttgt gaacatcaga agtaacatca tcttctgttc cagctagaac
    10621 accaagttct gcttcaacaa caacaccttt agagtgtgca tattcaacag cttctttagt
    10681 tattctaaca ttttcttcaa agtcaaaatg agaaccatct atcataactg aagagaaacc
    10741 agcatcaata caagttttaa tagcatccat atttggacca tggtctaagt gaagagctac
    10801 atctacacct atttcatcag aagcagcctt aaccatttca actaatgtat gaggtccagc
    10861 atatttaaca gctgacatag aagcttgtat cataacataa gaatttttag ctttagcagc
    10921 ttttaaaaca ccttgtaatt gttctaagtc acttatgtta aatgcaccta tagcaaatcc
    10981 accttcgtaa gcttttttaa acatttcttt tgtagtaatt aatgccattt taaactcctc
    11041 ctattaactt ttaaaattta cccatcactt atattataac gttaaatcaa aaaaagtata
    11101 tagtaaattg taataactat aaattacaag tataatgtta aatagaagac aataggatta
    11161 aataaaataa taaagaatat aaacaattta ggaaaactag ctatataatg ctaactataa
    11221 gggtaggaac taccttatta gtttggagat actgataaca atagttgcct tgaccaagaa
    11281 gttaccactt caaacgttat cgaaaggaat taagtggatg ataattcact aaaaatttaa
    11341 aataaaggag ataaactatg ccacagataa aaataagagg aattaatgaa aatgatatat
    11401 gtaaaataag tgaaaaaatg ataaatgatt tagttgaagc tgtaaaatgt ccaagagact
    11461 actttgaaat agaatgtata aagtcagttg caataagaga tggtaaaata gcagatgtat
    11521 atccttttgt tgaagtagct tggtttgata gaggacaaga agtacaagat atagtagcaa
    11581 gaataataac tgatagtata agaaataatt tagatgtaga aagtatagat ttagcattta
    11641 cagtatttga aaaagaaaaa tattatgaga atggagagca tttttaatgg ctaaaaagca
    11701 acgtatagat aaaattcttt caaatttagg ttatggaagt agaagtgaaa taaagaagta
    11761 ttgtaaacaa gggtctgtag tagtaaatgg aagtgaagtt tcaaatccag gaactcaagt
    11821 agacactgaa aatgatgaga tattatttaa tggagaagaa gttatatata gagaatatat
    11881 atatttgatg atgaataaac ctgatggata tatatcagca actacagata aatatgaccc
    11941 aacagtatta gatttaattg atttatcata cttagctttt gaaccatttc ctgttggaag
    12001 gttagataaa gacacagagg gattattagt tttaactaat gatggaaagc tttctcatag
    12061 agttttatct cctaagaagc atgtgcctaa gacttattat gcaaaaattg atggagtagt
    12121 tactgaagaa gatgtagaag cttttttaga aggtgtagtg cttgatgacg gatataaaac
    12181 tatgccatca cagttaaata tattaaaaag tgatgatgaa tcagaaatag agcttacaat
    12241 acatgaaggt aagtttcacc aagtaaaaag aatgtttgaa agcgtaggca aaaaagtagt
    12301 atacttaaaa agattgtcta tgggcaattt aaaattggat gaaagtttag agttaggtga
    12361 gtatagagag ttaacagatg aagaagtaaa attgattgaa gaaagataat aaagatacta
    12421 tagtaaattg gaggaatgtc tgtgaagaaa aaagatgtta tagaatttga agtagataaa
    12481 atggaatttg gtggtacatc tttaagccaa gttggagata gagttatcca tatgaagggt
    12541 ggaataagtg gtcaaaaggt gagagctgga gttaaaaaag ttagaagtaa gaaagctgaa
    12601 gttaagatga tagaattatt agagaactca ccacttgaaa ctgaagaaac ttgcaagcac
    12661 tttagagagt gtggaggatg tacactattg tctgtgccat atgaaaaaca attagaaata
    12721 aaagaaaaac aagttatgga tttattttta aagcaagatt tatttggatt tcagttttta
    12781 gggatagaag gaagtccaga aaacaaatat tatagaaata agatggaata tacttttgga
    12841 gatgaagtaa aaaatggacc tttgacatta ggtcttcata aaaaaggaaa gcatatagat
    12901 atacaaactg ttgaggagtg tatgttggtt gatgaagact tctcaaagat attagtttct
    12961 agtgtagagt ttttcaatga aaaaaagctt ccttattata gaacaatgaa tcacaaagga
    13021 tatttgagac atcttgtagt tagaaaaggg attcatacta atgagattat ggttaatata
    13081 gtaacttctt ctcaagaaga ttttgatatg tatgagttta aagatatgct tttagggatt
    13141 gatttaaaag ctgagttagt aggtgtactt catactatta atgatggttt agcagatgca
    13201 gtccaatgtg atgagttaag agtattgtat ggaagagatt atatacaaga agaaatttta
    13261 ggtcttaagt ttaagatttc accattttcg tttttccaaa ccaatactaa gggagcagag
    13321 gttctttata gtattgctag ggattttatt ggagattaca atgataaagt tgtatttgac
    13381 ttgtatagtg gtactgggtc tattggacag gttatggctg gtgctgctaa gaaagtgtat
    13441 ggtattgaga tagttgagga agctgttgtt gctgctaatg agaatgctaa gttgaatgga
    13501 cttactaatt gtgagtttat tgctggggat gtggctaagg ttgttaagga tttaaaggac
    13561 aagcctgatt tgattattgt tgaccctcct agaccaggca ttcataagga tgctattagg
    13621 gatatttgtg gatttggtgc taaggagatt gtttatattt cttgtaatcc taagtctttg
    13681 gttgttgatt tggttgattt taaggggtat ggttatgaga ttaagatggt taagtgtatg
    13741 gatatgttcc caaatacgcc acattgtgag actgtagtgt tgctacgtcg aaaataatgc
    13801 tagaaatctt gagctttaga cagtttcata gacatataat atttagatta gagggtaata
    13861 aaattaggaa taaagaattg aaaaggtgtt ttgatgtgtc tgtagttata tatatggagt
    13921 gtgggaatac gaaataaaaa aagttttaat tatagaaata gatattaaat ttataaattt
    13981 aatatctatt ttttatgtga aaatatattt aatctaaaga atgatatttc ataaaaaaaa
    14041 ttaaatatat ttttaaaata tttacaatag agttattttt tattcgtata ataatagaaa
    14101 tattttagca taagatgtat aataaaggaa aaaatacaga attaaaaaac ataagataga
    14161 taagtggact taggggagag aaattattat gcaattgaca aaaataattc ttagtaacta
    14221 taggtgtttt ggagaagaac ctacaactat tgaaattaat aatttaactg gatttatagg
    14281 tcataatagc agtggtaaaa ctgctttatt aagtgcactt atgaaacttt ttggagaaaa
    14341 gtctagtgat agaattattg aaagatcaga tttccatata cctgaaagta ttgatccaga
    14401 tagtatttct aagaatgaat tatatattga agcacaattt gagtttcctg aattggagag
    14461 aggtaaagac agcatatcta taccagtatt ttttaaaaat atggttgtta gtgaaccagg
    14521 tgggaaacct tatttaagaa ttagacttga agcaaattgg gagaaaagct caaatcctga
    14581 aggaagtatt gattcaaatg tatactatat aacatctaca acagaggaga taaatcctga
    14641 agataagcgt aatatcaaaa gatatgagct tgatcaaata aagatgattt atgtacctgc
    14701 cattaggaga ccagatgaac agttaaaaaa tgtctcagga acaataattt caagattatt
    14761 aaatggtata agttggtcag aacaaacaaa ggcaagaatt aaaacgaaag caagtgaaac
    14821 ggaggaaatt ttctttgaag aaaaaggggt tgaatcatta aagaaatcac tcgaaactca
    14881 atggaatagt tatcataagg atattagata ttctaatcca aagataaact tcaatagtac
    14941 tgatttagag acaattttaa aaaaagttga agtaactttt tcacctacag ggacacatcg
    15001 agattatcgg gttgaagagc ttggagatgg gttacgttct ttattttatc tttcacttgt
    15061 aaatacattt ttagaaattg aagaagaaat tctaaaatct attaatgatg gtaaagaaga
    15121 agatatattt acattaatac cgcctgtatt aacaattgtt gcggttgaag aaccagaaaa
    15181 tcatatttca cctcatcttt taggaagggt agttgaaaaa ttaaatttta tttctacaaa
    15241 agaaaattca cagacaatat tgacatcaca ttcaccagca atagtaaaaa gaattgaacc
    15301 aacagacata aggtatttta ggatttgtaa ggaatatgaa tgtacaaaag tgaagtcaat
    15361 tatattacca aatgaagaaa aggaagcaga acaatttaaa tttgtaaagg aatcagtatt
    15421 agcatatcct gaactttatt ttgcaaaact tgttgtattg ggcgaaggtg atagtgaaga
    15481 gatcattatt aagcagatga ttgaattaaa agcagaatct gtagattcat cagaaatttc
    15541 aatagtacca ttaggtggta ggcatgttaa tcatttttgg cgtttattaa atgatttaga
    15601 aattccattt attactttgt tagatttaga tagagaacga gatggaggtg gttggggacg
    15661 tataaagtat gcaatacaac aattaataaa tattggtcat gataagaatg tattacttta
    15721 tacatgtgaa ggaatttcac aagataatga atttaaagag atgcatactt gggatgtaac
    15781 tgatactgaa aatatggcta aatggattga aatgctaaaa ggttatgatg tttatttttc
    15841 agctccgtta gatatagatt ttgcaatgtt acaaagtttt aaagataaat atatttcagc
    15901 attaacaagt gcggaaggtc ctaggataaa agatcatgga aaaatacaag atattgtgat
    15961 caatgaagat gctgaagatg aatcattgaa agctgcttat aaagatagaa tagatagtga
    16021 tattaaatca accttaaaac aagaaggtgg tgatggatct acttatactg agggagagaa
    16081 agaactaatg atatggtatt catatttctt tttaggaaga ggtaaaccaa ctactcattt
    16141 atctttattt tctaataata ctgatataga ttttgaaagt tttccatact gtctaaagga
    16201 gtttgtaaat agtattattg aaaagatgat agatgattca gggggtggaa gaaaatgaag
    16261 aggaattggg ttagtaaaga agattggagt ccagcggctg gattcagact agaagttgaa
    16321 gcgatgaata cagttattaa tgataagaat acacttgttg tagctggtcc tggcgctgga
    16381 aaaacagagt tattagctca gagagcctgc tttttactag agactaatac ctgtagatat
    16441 cctaaaaaaa tccttgctat tagttttaaa aaggatgctg ctgaaaattt aaaagagcgt
    16501 ttagaattga gatgtgggaa agaattagct tcaagatttg aatcaaagac ctatgatgct
    16561 tttgcaaaag aaatcgtaga tagatttaga aatagtttag atgaagcata tagaccttta
    16621 aataattaca atatagctga aaatcgtgat gttagaagag catttgagat agcaggatta
    16681 aatttcagag gaaatcaaac tgaatttaat aaagtatatg gaaataaact atcgataaat
    16741 aaattgcctt tacatacaga ttttgtagat aagattttta tagatgcttg gaatgtacta
    16801 cttaaaggca acaaagatac taattttgta agttcactta cttttggtat gatatcacgt
    16861 ttagccgaat atttgataag aaccaatagg tatattaaga aagcaattga aatgacttat
    16921 agtcatgtgt ttttagatga gtttcaagat actaaatcta ttcaatatga tcttgttaaa
    16981 gcttgttttt ttaaaacaga aacaataatt actgctgtag gggatggcaa acaacgaatt
    17041 atgttatggg ctggtgctag aaagtcaata tttgaagatt ttcaaaatga ttttttaagt
    17101 caaaaaagaa cattaattat gaatcatcgt tcagcgccaa gacttgtaga gattcaaaaa
    17161 gcaatgtata acagtttgaa tgaagaggca attgatttaa aaactaatga aaagtggaat
    17221 tatgaagatg gagaagtgta tttaagactt tttgaggatt atataaaaga agctgatata
    17281 cttagtgagg aaatagtcga attggtggaa acaggtatag aaattaatga aatatgtata
    17341 ttagtaaaac agagcgtaga tatttattca aaagagatta taaataaact ttctgaacgt
    17401 aatattaagg cgcgtaatga agcaatgtat caggagatat taaaagaaga tataataaag
    17461 acactggttt cgctttttag gttgtcgtta agtgatagaa atcccgatga ttgggattat
    17521 atttacgata ttctaggtga attatatgga atagacgaag ggtataaccc taaaatatta
    17581 aatgatttca ttaccaaagt agaattaatg attaatgatc tcaaggtaaa tttaaatatt
    17641 actgatgaaa aacaattttc agatttagtt gattctaagg ttgaatatat ttcatatgaa
    17701 aaatttgcat caaagtttca aaaatataaa tctaaagatt attttgatga tttggtagat
    17761 aaatttaaaa agctggtatg gattgaatat aatgaaacta aagattggat taaagcgatt
    17821 gataactttg aaggtaaaaa ttcaattcca ataatgacaa ttcataaaag taaaggactt
    17881 gaatatgata ctatcttttt tgttgggtta gaagattcag cattttggaa ttttaaaaat
    17941 caaccagatg aagatagatg tgcatttttt gttgcattat caagagctaa aagaagaatt
    18001 gattttacat ttagcttaga tagaccagca ggattttcga attcacagaa gcattattgt
    18061 attaatgaat tctacgaatt gtttgataat actgagattg taaaaaagat agagtattga
    18121 ttaagatgat aaaaacttta gtatattata ttttaagata ttagttatat aagtagacat
    18181 tgaatttatg tatttgatgt ctatttttta tataaaaata tattaaagaa aggatgaatt
    18241 tgcatgaaga aaaaaacaaa aatatctaaa aagattgtaa gttttattat ggtttttata
    18301 actatagtta ctactatact tacatcatca gtttcagctt atgctagcga gttggatatg
    18361 agtgaacaga caggctttaa ttatttaggt attagtccaa ttacaggaaa aaaattaatc
    18421 ataacattta tcgtatgaag atggataata aaattgtatt ttgtgtagag ccaggaatta
    18481 aaacttcaag tggaggagga tatatatcgg aggagttagg agataaattt atatctacaa
    18541 cacttccaaa ttatcataaa agaaaagctg aaatacttga gaaggtaaat agacataata
    18601 tattaccttc atggaataat gaaaaagttg cagttaaaag tggagaaaca attgagttag
    18661 aagataagaa tggtattatt agagaaatgc aattagaatc aaataatact ggaacagata
    18721 tattacttga agaaaatact ttgaaaatta cagcaaataa aaattctaat tcaggagtta
    18781 ttagttatat aaagattcct aaaaatgagg ttggaatatc aatagtttat cgtaagcata
    18841 actatcaaag cttatcagaa tttcatatgg aaagttcgat gagtgcaaat ttaaatataa
    18901 atgtaatcaa gttaggaatt gttaaagtaa aaaagattga tgaaggcaca ggaaaggaat
    18961 tagcaaatgc aaaacttaga tttgagtatg atggtgatag taaagaaatt ataacgggtt
    19021 ctaatgaaat tgctagtata gaagatatta ttggttgatt aaaaagtgaa gtatactttt
    19081 gtatctatga aaaagactat aagcaatata taaaattaga tatacctatt gatgaagcaa
    19141 aaataaaaaa tagatttgaa ataaggttaa aaaatgaaag agcatatcat gcagcagttg
    19201 atttgttagc aaatagagat gaagaaaaga cagcattttc aattattaat cgttatatac
    19261 gttttgttga taaagatgat gaaaaaatta gaagtgaatg gaaaactaat gatcagtgga
    19321 gttattttat tggtgaaggt agagcgaagt taaaactaac aacaaatcca gaaccatata
    19381 atattcaaaa aactttaaat tggttatcaa gacaagttgc accaacgtta aaaacaatta
    19441 aaaggttaga tagtttgaac aactcaacat taattgaggg cttaataagg tatgcaagac
    19501 taacagataa acataaaaaa ataattgaac aaaattcagt aaatgttgaa gacattattt
    19561 atgatgaata aaaaaatgta attagtattg cagattttaa tataaaagaa gggagatata
    19621 cgaatgaatt ttggtcaaaa tttatataat tagtttttat caaatgcaca atcattggta
    19681 ttaatggcta tagctataat tggtgtatat ttaggattta agagagagtt ttcaaaactt
    19741 ataggatttt tagtagttgc attaattgca gtagggcttg tgtttaatac agcaggagtt
    19801 aaagatatat tattggagct atttaataaa ataataggtg tgtaaggatt aaaagagaat
    19861 agaaaattat gagtatatga tgaagggttc taataagttt tattaaaata tatattgaaa
    19921 gacaagtaga gctatttcga gttattacat gtaatgctaa tatatataac tgcattttgg
    19981 aaattaaata cataattaga aatagcaaat tttataggtg acttattaat atgtttactt
    20041 attgtatact atttgatata tttaatatga ggtgattctt gaaaatggac atagtgaaag
    20101 tttttgctaa caatgtgaga aaatatagaa ataaaaaagg tctatcacaa gaagcttttg
    20161 ctgaaaaagc tggattgcat agaacttata tttgtgctat tgaaagaaaa aagagaagta
    20221 ttgctttaga taatgtgcag aaaattgctg atgcgcttga gattgagact tacttactat
    20281 ttatagaagg tgaggagaaa gaatgtctat gaaaaaatta ccaatagaaa agcttataga
    20341 aaatttaggt ttttcagggt caaagaatct tgtgaatata gatatgctta caaaattaga
    20401 tatatctaaa catgatgtaa aaactttaaa agttataaat ccttatgcaa tttatttagt
    20461 aaatagtaag ccatttatat tatttataga gtcttctttt gaagaaaaaa aattacaaca
    20521 aattagtaag cttatatgga atgctcaaat accaattgct tttatatgcg attctaattc
    20581 tgttaaaata tttaatggaa aagctttgga tttaaagtca tatttaattg aggaaatata
    20641 taatcaatct attgaggatt tttcggaaga atctgagttt tcttatttga aaatatctga
    20701 tccattattt tgggatcaat acactaaaca atataccaaa acgcatttaa atgattattt
    20761 gctagataat attacttcat tgacaaataa attaaagaaa aaatataagg tgaaatttgc
    20821 aacgaagctt gttctaagat taatatttat tagatattta atcgatagag gtgtcgatat
    20881 agcttataaa aattttaatc atgatattaa agcttcacaa aaagaactgc taaagattac
    20941 taaaaacaaa gttgaattat atgaactgtt taaatactta aaagaaaagt ttaatggtaa
    21001 tttatttgag ttaaatgacg aaattaataa tacttgttta acagatgaag tgtttatttt
    21061 actatctgat tttttatcag gtaaaataag tattgacaat ggacaaatgt ctttattttc
    21121 aatgtatgac tttaatatca taccagttga actaattagt aatatatacg aaattctttt
    21181 aggaaaagaa attcgtaata aagataacgc attctatact ccgaattact tagcagaata
    21241 tattttagat aaaacaacac ttgaatttct gaaaaataat aggcaataca aaattctaga
    21301 tccttcttgt ggatcaggag tatttttagt aaatagtttt agacgtatta ttgatatgaa
    21361 tttgggtgat aaactttttt gtgaagatga taaactttta aaagaacttt tattatctaa
    21421 tatttatgga attgatatca atgaagatgc tatcgatgtt actattttct cgttatatct
    21481 aactatttta gattacaaag accctaagat attatcagaa tttaaactgc ctaatttaaa
    21541 agggtgtaat ttaatagcga gtgatttttt tgatgataat aaattggaga gtttaaagaa
    21601 aattaacttt gattttatca ttggaaatcc gccttgggga agtgtctcag atggattaca
    21661 tttagattat tgcaataaaa atggctataa tgatatgcaa caaaacaaag aaatcagtag
    21721 aagttttatt tttagagcaa aagattttag ctctagaaat acagtatgtt cttttgtagt
    21781 gcattctaaa atactatata ctcaaaaaaa gccatcaaaa aaattccgtg aatttttgct
    21841 taaaaaaagt caaatattta gtgtagttga aatgtcatct gtaagaaaat tagtgtttaa
    21901 aaatgcagac gctcctgcag cagttatatc atttaagtat aacgaaagtg aagaaaattt
    21961 aaataataga atcatctata catctttaaa accaaatgta ttttttaaat tatttaacat
    22021 tattgtaatt gaaaaatatg atgttaaata tatttcacaa aaactattat tggagaatga
    22081 ttgggcttgg aaaactattg tgtatggatt cagtaaagat atagatataa ttatgaatat
    22141 caaaaaaaaa tatcaaactg taaatgaaat tattttgaaa aacagttttg ttcatggaac
    22201 agggatacaa atcaatggta atgaaagcca tgatgcgaca catttaaaag gaaagtggta
    22261 tgttgattca aagaaaggaa tagattcatt ctatgttgat ataaataaag ggaaagaact
    22321 tactcaagat aaaatgagaa gacctaaggc taatcagcaa gaaatattta ttcctccata
    22381 tgtattatta aaaaagggag ttgacacgaa aacatataga tatagagcag cattttcaaa
    22441 tgatgcattt gtatattcag atgcagttac aggaatatgt ggaaaaaaag ataatttaac
    22501 tacactttat tgcttgactg gtatgcttaa ctcttctttt tactcatact taaatataat
    22561 gctaggtaca tcaattggaa tagaaagaga acaaggattt tcaacggaaa tttttacgta
    22621 tccagcaata gaaagtgatg aaattgctaa tctggtagta aaaatacaag aagcgactaa
    22681 attagaaaaa acaactatgt tcttgaatga aaaaaatagt tgtaagtata tcaaacaatt
    22741 agataagtta attttgaagt cttttaattt aaatgatgat aaatttattg actatgcaat
    22801 aaatattcaa attcctgagc ttacaaattc taagtcaact aacatatata gaaaaataac
    22861 taaggaagat ttaattaatt atagtgaatg tttcaaagaa cagttttcat cgatatatcg
    22921 tagggttggg aaaagtattt caatttcggt atatccaaaa atattaaatc gttttacagt
    22981 atttgaacta gaagtaacag atgaaaaaac agatagagag attatggtta aagcagaagc
    23041 tgataataat atggagttgt ttacgaaaat aattagatat gattataatg atgtttttca
    23101 tcaaattaga gatattattc attttggaga aaattcattt tatatcataa agcctaattg
    23161 ctataaatat tggcatcctg ctatcgcaga aatggatctt gctgatgttt tggatcagat
    23221 tttatctgat acagggggtg aagaataatg ggaggattta aaaattcttc actaaacttt
    23281 tatttaaagc aagggcttta tgaaataatt atagatggat taatagactg ttgtttactt
    23341 atgagcaaat catgtattga acaatctaga aaaatattaa atcacgaaga aaagataaga
    23401 acgcatttag ttgaaaatta tttagaaaat gatattgaaa gaagaaaaac atctttatct
    23461 aaacaacatc taagatttga agtagaagca caagaaaatt atgacaacaa aaatgaatct
    23521 tatgagggta gggttgatat aaaagttata tctaaagaca ctttagcgga tagaagggct
    23581 tattatatca ttgaatgtaa aaggatagat ggtaaggaaa ctttaaataa aaaatatata
    23641 accgaaggtg tacaacgatt tattactgag aaatattctt cgcattatgg caaaaatata
    23701 atgcttggat ttatagtaaa agatataaat atccagagca atatacaaaa aattaattca
    23761 ctgaatcaga acatattata tacatatata gatacagata tgttgacagt tagaagtgat
    23821 tctgaaaata attatgctgt ttgtgaaagt aaatataaag taaaatctaa actactattg
    23881 ttaaatcata ttttttataa cttttcgagt ataataagat aatcttatga atataaaata
    23941 ttactatgtt aaaattatag tttggttttg ataatattat aaaaataagt ttaagaataa
    24001 aatataaagg aaaaaagggg cgtagcacta ttttttagtg tgacagcccc tttaatttta
    24061 aaatcggtaa ggaggagtgt atttgaaaaa aattaagtca tatacaggga ttttgaatgt
    24121 ggagaaggtt atatatgcaa taaatgattt tcagcttcca tttccagtta cttttacgca
    24181 gatgacatgg tttgtattaa cactttttat aattatatta tttggtaagt tacctccatt
    24241 atttatgata gaaggagcat ttttaaagta tcttggcata ccaattttta taacttagtt
    24301 tatgtctcaa aagacttttg atggaaagaa accatatgga tttttaaagt caataatact
    24361 atattcatta agacctaaaa taacatacgc aggaaaaaac attaaattta aaaataaaat
    24421 tattgatgaa aatataacta tagtaaggag tgaattatat gttcccgata aaatatatag
    24481 agaacaatct tgtatttaat aaggaaggag aatgttttgc atattatgaa ttaataccat
    24541 ataattattc attcttataa gctgaacaaa aatttattgt acatgataat tttaggcaat
    24601 taatatcaca aagtagaaat ggtaaaattc atgcacttca aatagccact gaaagttcta
    24661 taagagttac acaagaaagg ttaaagaaaa atattacagg aaaattaaaa gaaattgctt
    24721 atgaaaaaat agacgagcaa acagaggcac ttgtttctat gattggcgat aatcaaattg
    24781 attatcgttt ttttattggg tttaagcttt tagttacaga gaaagaaatg aatataaaga
    24841 gtataaaaaa gtcaatatta gctaatctat cagattttat atatgatgtt aataataaat
    24901 tgatggatga ctttgtcagt ataaataatg atgaaattat gagattttta aaaatggaaa
    24961 agttattaga aaataaaatc tcaagaagat ttaaagttag aaggttagat aaaaatgatt
    25021 ttggatattt aattgaacat aattatggaa agaaaggcac agtttttgaa gattatcaat
    25081 accatttacc tacaaagaaa cttaaaagag aaacattagt aaagaaatac aatcttataa
    25141 agccaactag atgtcttata gaagagaagc aaagatactt aaaaattata catgaggatt
    25201 cagaaagttt tgtagcttat tttactattg aaattattat tggagaaatg aattttccat
    25261 ctagtgagat attctattat cagcaacaac agtttgcatt tcctattgat actagtatga
    25321 atgtggaaat tgtaacaaat aaaaaggctt taactacagt tagaaataag aaaaaagaat
    25381 taaaagattt agacaatcat gcttggcagt cagggaatga aacagtaaca aatgtagtag
    25441 aagcattaga ggctgtaaat gagcttgtaa ataatttaaa tcaaacaaaa gaatcaatgt
    25501 ataagttaag ctatgtaatt agggtatcag cagatagcat tgatgaatta aaacgccgtt
    25561 gtgatgaagt taaagatttt tatgacgatt taaatataaa attagtacgc ctaatgggag
    25621 atatgatagg atttcacaat gaatttatac cttcaagcaa aaggtatata aatgattata
    25681 ttcaatatgt aactagtgat tttctagcag gattagggtt tggagcaact caatagcttg
    25741 gagagaacga aggaatatat attggatata gtttagatac aggaagaaat gtctatcttc
    25801 aaccatctct tgcaagtcaa ggagtaaaag aatctgtaac taatgcgttg gtagcagcat
    25861 tctttggatt tttaggaggt gggaaatcat ttgctaataa cttacttgtt tactattcag
    25921 tattgtttgg agggcaagct gttttggtgg atccaaaagc tgaacgtggc aaatggaaag
    25981 aaaatttacc agatatatat catgaagtaa atattgttaa tttaacaagt gatatagaaa
    26041 ataagggaat gctagatcct tatgtaatta tgaaagaagt aaaagatagt ggaagtctag
    26101 ctattgatat attaactttt cttacaggaa taagtagtca tgatggagag cagtttccta
    26161 tacttagaaa atcaatcata aatgttactc aagacaaatg cagaggactt ttgaaggtaa
    26221 tagatgaatt aaggaaagat aattctcaaa tatcaaataa tattgcagat catattgaaa
    26281 gttttacaga ttatgatttt gcacaattac ttttctcaga tggaaatatt aaaggctcaa
    26341 ttaatctaga aaaacaaatg aatataatac aagttgcaga tcttgtttta ccgaataaag
    26401 atactttatt tgaagaatat acaacaatgg aaagtctttc agtagctatg ttgatagtaa
    26461 ttagtacttt tgcattagat tttatccata cagatagaag tacttttaaa atagtagatt
    26521 tagatgaagc ttggacattt cttaatgtag cacaaggaaa aactttatct aataaattag
    26581 ttagagcagg atgtgctatg aatgcaggag tgtattttgt aactcaaaat gcaggtgatg
    26641 tatcagatga aaatttaaaa aacaatattg gattaaaatt tgcattcaga agcacagata
    26701 tcaatgaaat taagagaata cttgaatttt ttggagttga taaggaagat gagaataatc
    26761 aaaaaaggtt aagagagtta gagaatggac agtgtttaat acaagattta tatggacgag
    26821 ttggggtaat acaaatacat ctagtttttc aagaactcct tactgcattt gatacaagac
    26881 cacctataag aaaagaggtg gattaatgaa atcgaaagta tttagaaatt taagctggat
    26941 aactatttta atagtagtac ttttactatt ttttatttat ataaatttat agatttagag
    27001 aaacaaatag atacattaga taagaaaaga aaaaaactca tagatatgag attagaagat
    27061 ataattgata aagaatcata tgaagaaaag tatgatgatt taacagttga aataaagaaa
    27121 cttagtaaag agaaaaaaac tcttaaagca tcattaataa atgaaaagag tataaaagca
    27181 agacttagag aatttagaaa gatacttgaa agaaatgaat tgttagaaaa atttgataga
    27241 tatgtatttg caagtatagt agataaggtt atagttggag ttattaatga agatggtata
    27301 gttgatcctt ataaaataac ttttgtttat aaaactagct ttgatgataa acaagatgga
    27361 cataaattta taaataagag tccgttagaa acgtattcaa aagataacaa cgataataaa
    27421 aagaagtgtt catatactaa taatgagaca ttgcgagact gtagttaaac ttattagaga
    27481 ataacaaggt ttcagacttt tatattgtgt cttaaaagta gatttgccac ctatttgcca
    27541 ccataattat ttttgggtgg caaatttatt ctacaatgat ttcaaaaata tttaccgtct
    27601 ggtttctcat gttgttagtt acatgtaaat atgtatccat agttgttgaa gttttagcat
    27661 gtccgagtat atgctggata tcttttatat tagtaccatt ttctaataac atagtatcag
    27721 ggtttttaaa ataaagtgtg tattcttcta aagtagtgat aattaaaata tcaaatatca
    27781 ctatacaaag gaaagtgcac gctttttatg tttaacaaaa acgaaatatt aaaaaactta
    27841 ttgaagaata tgatgtcaaa acaactacag atattcaaga tatgctaaaa gatttattta
    27901 caaatacaat tcatcaaata tttacattgt cgaattttag cggtttaaac ataatatatt
    27961 gactgatata ataaaatcat agaatatttt agggggaaat agattatgtt taagaaaaag
    28021 ttaatagctg ttgcaatgag tataactatg attagtgtag ggagcttttc gtatgctcat
    28081 tcaggaagaa ctgattcatc aggaggtcat cgtgataata aaaataaatc tggattagga
    28141 agttatcact atcattgtgg tggttatcca gctcatttac ataataatgg tggttgtcct
    28201 tatactggag gaggaagcag ttctggaaca acaacttcgg ttaataatga agaaaagcaa
    28261 aagaggtctg ttggagaaaa aggatataat caagggtatg aagatgggta taaaggaaat
    28321 tatagttcat caaattatag tggagattat agtgatacat atgaatcaaa gtactcagaa
    28381 ggatatgaaa aaggtaaggc taaattagaa gaggaagaaa aggtggctaa agagactgga
    28441 tataatttag ggcttacagg tgctaaatct aataatacat atgaaaaaga agttttaaaa
    28501 aatgcttatg ataccggata ttcaactgga tacaatgaat ataagactaa aaagatagaa
    28561 gaatataaag ctaaagggat agaggatagc aataaagata aggaaaaaat gacatttgaa
    28621 gaaaacatag attctgaatt tatagatgct tataataatg cttatgacga aatacaagaa
    28681 caattaaaaa atgattacac tactcaagga tttgagagtg ctatcaaagg agaaagattt
    28741 gacacatcaa ctataggtaa tgttaaatat gctaattggt ttaaagaagg atatgataat
    28801 ggtaaggtta agttacctaa agcaaaagaa agtgtatata atcaaggtta caatgaagaa
    28861 gattttagta taccagatga aatgaagtct attgaaacta agttaaaagg tgtatatgat
    28921 gaaggtttag aaaaaagaga agaagagaaa agtagaaatg ctacttatgg ggttggagct
    28981 ggaacagcag ctgtagttgc tggagtagta tataagaaga aaaaacgtaa aaagatatag
    29041 atgctagata taccaatgat gacaagtgta taacaaatat aattttaagt ataaatattt
    29101 aaaatataga agggaaggtt atatctatgg gatttaactt taggaaaagc ataaatctag
    29161 gaggaggact caaattaaat ttaggtaaga agagtgtagg tataagtgct ggtgttaaag
    29221 gtgctagagt gtcagttaat tcaaaaggaa gaaaatcagc tacattgagt atacctggaa
    29281 ctggaatatc atatacaaaa actagtacaa agaaacgtaa acctaaataa atatttgata
    29341 tactaagaaa tagactgtta caaatagtct attttttagt atatagattt aaaaagtgac
    29401 aaaattttga aatataaact aatttcataa aggaaaatat tattttatgt agaattataa
    29461 tatttaagta atattataaa atttatagga ggatttcatt atgaaattaa gcaaaatact
    29521 attggcaata gggatgtctg tttcattatt gattgctagt tgtccaacat caaacgctct
    29581 tagttctatt aattcaataa aaggtacaaa taagtatgaa acagcaggtt taatagcaga
    29641 taaacaaaat tatgatattg caatacttat taattctgaa aatggattat ctgatggatt
    29701 gtcagcaagt ggattgtcag gagctaaaaa tgccccaata ttgttaacta agaaagatac
    29761 aatcccttca gaaacattaa aaagattaga aaaggtaaaa gaagtatatc taattggagg
    29821 aaaaaattcg ataggagcat cagttcagaa aatattaagt gataaaaaaa taagcataaa
    29881 tagaataagt ggaactgata gaatagatac aagtcataaa gttgcaaaag aaataggaag
    29941 agtaagtacg gttaaaaaag taatattaac taatgctttt aaaggcgaac cagatgctat
    30001 gagtgttgct tctatagcag ttagagataa atctccaata gtattaactg atggaaatac
    30061 tacaagtttt gctacagatg gattagaaac ttatgcaatt ggtggaaaag tcagtatgag
    30121 tgataagtta ataaatgaaa ctaaagcaac aagaataggt ggagtagata gatacgatac
    30181 aaataaacaa ataataaata aattttataa tggctcaaaa gaattttaca tagcaagtgg
    30241 gactgattta gtttattctt tagttggttc tacaattgca caaaaaacac ctatagtgtt
    30301 agttggaaat ggaagtaata agagtatatt aaaaaatgca agtaaaataa ctgctattgg
    30361 aaatattgat gatgttgtta taaaacaatg tttaaatata actaataatg ttggggattc
    30421 aaatacaggt attgtaaaag atactaattc aaataatacg aacaaaccaa gtaatgagcc
    30481 tagctctaat aatcctggat taactgataa aagtacagta tatgtaactc ctaatggaaa
    30541 atcatatcat ctaacaaaaa attgttctac actaaaaaga agtaaaacaa taaataaagt
    30601 aacattaaaa caagctaagt cacaaggtaa aatagatcct tgtaatgttt gtgtaaaata
    30661 atgtattagc caaatatata ttaaaaaaga caattaatta agattgtctt ttttattgaa
    30721 gaaatataaa tttatattat gtattattaa aaaaaaatta ttaattataa aaaataataa
    30781 taaagacgat aagtattaaa attttatgtt tccaatattt atgatataat taatatggca
    30841 aggaataaat atttgaaaaa gctatgagtg gtgtttccat ggaagtttct tcactttctt
    30901 atgagaggag gtgaaaacta tggaaaatct aatgatgagt attataactg gtgttatagc
    30961 cagttacatt tacgataaat taaagagcca ctctgagcga ccaactaaga gtggctggga
    31021 acttaatata aagttccaga aaagtaaatc ttcaaaataa tttgttgaag gaaacatcac
    31081 tcaaagttaa gtaaaatatg tttccttgct tttattatat cacaaattaa aaatattaac
    31141 acaaaatttt gtgaatatat acttattatt gttctaaaaa aatatgtaaa taattgattt
    31201 gattgcatgg aattattgat gtaatattta agttaaatat acacaatctc aattaaagaa
    31261 gataagtact ataaattcat atcaaaaaaa tacataaact tttaagttat aattttataa
    31321 aaagtctttt agtatcatta tttcaagagg aattatcata tgaaaaaata acttcattag
    31381 aaaagtggat aaaaaactgg taagaagatg actaaagatt acatatacta ttacttaaat
    31441 taaaaattta atataattta taatgtatac aatttattat aaaaaattta attttaattt
    31501 tctatttttg atactataat atataatctt gataatttaa ttaaaaatct agattttaca
    31561 taccaaactg gtttcttatt tcgctatctt tacttatgaa ctcatatatt cctataaagg
    31621 ttattttaga aaaagttggt tatttaaaca ttaataccac aatttctata gattgtttgg
    31681 tgaataatct ataattatat tatggactaa aaaatccaat gagctaattt attgtttttt
    31741 tatatttgag agattacaac
//
LOCUS       DAYAPA010000037        30680 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.037, whole genome shotgun sequence.
ACCESSION   DAYAPA010000037 DAYAPA010000000
VERSION     DAYAPA010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 30680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30680
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.037"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            103..2004
                     /locus_tag="ACGI59_001568"
     CDS             103..2004
                     /locus_tag="ACGI59_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFG9739357.1"
                     /translation="MRERIILETLKNSSGYLNIDYFADKLGVSTRTIRNEIKKIETIK
                     ECNGFKLEYKSKLGYILNIKDQDKFEHYLRNLPSDLVENPEQRLESIIVELLVNEGYK
                     TIEQLSKKFLVSSSQIKNDLKKIDEKIKDTELKLERKAHYGIKIEGSIKEIQSILVDS
                     YFRGNRNITEYRNKFIDNLKLANIRSTIKNVLNEHDLEANLTELEEILAQIIILYIRV
                     SMRVVGNASDLKLYTEDLIIDKLLDKIFRDKKYNLNYDEKYYLKELIKLKTKDKKATI
                     KNIDKNKLQDIMFEFFREIDKKYNSNFLEDKEFFNLFYLHIACLIERIKKNHKIINPF
                     SVKISQQYPTVFNLSIQFSKIIENEYHIKISQDEIGFIATHIAVPFEKREEANFNKKY
                     KIAIICSSGGGSAFLINLRLKEIFPNAEIRNFSLLEEKAVIEFAPDLIFSITNLLFEI
                     NAPVILINEILDELDYLDIKESVRFADHIGNISSPKQYILGLFDKNHFRCIKDKAKYK
                     DILDSMSKCIVDEGACSPTYPKDVWERESYLSTIYTNGVAIPHPIEMTGNKNIISVAL
                     IQTDIIYENRVPKIIFMISLIKGNLELHKQISKYLSKIMANKDMVDMLNKSQSYEEFM
                     YKLKIYLGG"
     gene            2007..3050
                     /gene="ypdE"
                     /locus_tag="ACGI59_001569"
     CDS             2007..3050
                     /gene="ypdE"
                     /locus_tag="ACGI59_001569"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFG9739358.1"
                     /translation="MNIEFFKRLSEADGIASNEEEVRHVLLEELKAYSDKIICDGLGS
                     IIFSKIKDESAPNVMICAHMDEVGFMVRSIDKLGMIHLITIGGVKPLAQFVQKVRITT
                     KEGKKIPAVINATYNNGKAENIYADIGAYTEEDVYNLGINVGDMVTYTTSFEEFTLPD
                     RLVGKAFDDRIGCFVMGEVLKELRKENLNCNIHFAATSSEEVGIRGAKTSTQLINPDI
                     VFVIDVACAKNEFVRDYTNKKQIDKGVMLMHRDRTLVPSRKMIDYVMEIADKNNIPLQ
                     HDMFESGGTDGGEAHLVNEGKPCVVTCVPVRYGHCAFSIVSNKDLENIIRLYTQLILN
                     FDEEQYKHIRNFI"
     gene            3065..3370
                     /locus_tag="ACGI59_001570"
     CDS             3065..3370
                     /locus_tag="ACGI59_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFG9739359.1"
                     /translation="MSTEVQIMELISTAGESKSKAFEALKKVKSKDFKGAKLLIEEAR
                     KIDIEAHRIQTELITQDLNEDVESPAMSLLMVHAQDHYMTSQLAKDLIEALIYCLDK"
     gene            3467..3802
                     /locus_tag="ACGI59_001571"
     CDS             3467..3802
                     /locus_tag="ACGI59_001571"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFG9739360.1"
                     /translation="MKILLCCAGGFSTNMLMQNMKKVVKESAKLNEKDFEFTAIPADS
                     IESEVDDWDIVLIGPQIAHKIDFLKGILEPKNKPFAVIDKDVYGKMDGATVMKLALIT
                     YKKHQLAQG"
     gene            3834..5156
                     /locus_tag="ACGI59_001572"
     CDS             3834..5156
                     /locus_tag="ACGI59_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFG9739361.1"
                     /translation="MFSKFEAFMNRVFMPLAHKVDNQRHLGSIKAGMVAMTPFTILGS
                     IFAILPALPNMLGENNPISKFILSNSAILDLPVSLSIGLIGLYACMCIAFNLGNYYKL
                     YIPGCVALSTFSFLFLVASFTPDGALEIGNLGAKGLFTGMIAGLGSVELYNFCNKKNI
                     TIKMPENVPDFVSRSFELIPTTIIVCGTFIVARFISLKVFGELPPQILTQFLSPLVGS
                     MDNPWSVLALNFAICTIFFFGIHSSVFSPITRPIMITFIAENIAAMQAGETIPHFFTA
                     GASSAFFGFTGCGISIGCVIACMLSKNASYKKIGRVSLFPALFGINEPILFGAPIILN
                     PIMFIPHVFGGAIIGTLPMFFMHWGWLAKPVFDPPYVGVFLEGFLTNGDWHSILANAL
                     QLVLAVLLYWPFFKIMERQQNNKKQKEETKNIFSKEEEGLLDDLGLDF"
     gene            5178..6236
                     /locus_tag="ACGI59_001573"
     CDS             5178..6236
                     /locus_tag="ACGI59_001573"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HFG9739362.1"
                     /translation="MERLVSLIYKRGVDALLIKAKNTKRYIGALSGSGVYVLVTKDKN
                     YQILDGRYIDEADKKTTGFIKRIVSQGSYIPTIIELLKELNIKKLAIENQAMSIQEYL
                     SLKNEGFEINLITNELWKLRAIKSKEEIKLIKKACEITDEVFDEVISEIKKDMTELEV
                     SALIQYHALKKGASGMSFETIVVSGERGAMPHGRPTNKKLKVNEAITIDFGVVYQGYQ
                     SDMTRTISIGKPPKIIKEIYDVVLEAQLSAIESIKEGTRASDVDKVARKIIDKHGFGE
                     YFNHGLGHGIGLGDGEVPTLNPNSEDILVEGMVMSCEPGIYIPNVGGVRIEDDIVIID
                     GKGVPLNKTSKEFIILGE"
     gene            6241..7428
                     /locus_tag="ACGI59_001574"
     CDS             6241..7428
                     /locus_tag="ACGI59_001574"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFG9739363.1"
                     /translation="MKYNFNKVLNRKEGNCRKWSNHVIKEKFGLNEDAIPMDLADIDF
                     ECAPAIKESILKRASVGDYSYTFIGDDFYDAIINWNKRRFDVYIEKDWIKLTFGTVST
                     LHYIVQAYTKEGEGVLINTPAYDPFAEAVVNNNRKLCCSSLKLDNNRYYLDLEDMENQ
                     MKYENIRLLIFCSPQNPSGRIWTKEELYQVSELCLKYNVILVSDEIHRDIIFKDYRFV
                     SLWNAHPEIYKNSIICVSPNKGFNLGGLKTSYVLIRDVKIRETLLERLKSNSITSPNV
                     FAIPAIVAAYNESEEWLDAMTSYVEENFEIVYDFFETNIPKAKVMKSDSSFLAWIDVR
                     EVFRNEEESKEFFRHANLTMVVGSYFVKDGDGFIRINVGCPRETLNEALNRIKKTYIS
                     MYC"
     gene            complement(7625..8497)
                     /locus_tag="ACGI59_001575"
     CDS             complement(7625..8497)
                     /locus_tag="ACGI59_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFG9739364.1"
                     /translation="MNILQNENLIIESSSFGAELTKIFSKKYDKNILWDGNKKYWGRQ
                     SPILFPIVGKLFDNETIIEENLYSMTQHGFARDMDFEIVDKGDTFVTYKLTDNESTLK
                     KYPYSFELIINYTLNDNSIEVEWIVKNTDSKDIFFSIGGHPAFNLPFYEQNDFSKHYL
                     EFKSKNDVEKINLNGSFTDDIIPIGKLKNLQLNPEVFKNDALIYTNIDEVSICNNDGS
                     KYVTISMEDFPLVGIWTPYYSETNSTAPFLCIEPWYGLADSINSNKIYKDKKFINKLS
                     KGKVFTASYNINIH"
     gene            complement(8891..9082)
                     /locus_tag="ACGI59_001576"
     CDS             complement(8891..9082)
                     /locus_tag="ACGI59_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9739365.1"
                     /translation="MDDFSKLKSLYEDGYRCIYHDCVDNNYTIYLKNFYTEGSETIEL
                     SSESDFSQFKDYIDGLRMS"
     gene            9302..10348
                     /gene="cobT"
                     /locus_tag="ACGI59_001577"
     CDS             9302..10348
                     /gene="cobT"
                     /locus_tag="ACGI59_001577"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HFG9739366.1"
                     /translation="MSLLESISKNIYSLDNSSIEKTKQRLDRLIHPTGSLGKIEDICM
                     QLAGIFGNENFDTSKKVIIAFAGDHGVYEEGVAPDPQNITKLQFPNFSKGLCGVGVIS
                     KFVGADVVAVDVGINCDEKLDGVLDYKIRKGTSNMAKGPAMSKQEAIRCLEIGIEIAE
                     QCIEKDYKVIGIGEMGIANTTPSTAIISVISGCDPLEVTGIGAGLKKERLKHKAEVIR
                     KAIEINNPNPTDGVDILSKVGGFEIGSMAGVILGCSANRIPVVIDGFISYAAALIAYK
                     INPKTREYMIASHLSAESGTKRALDILKLDPLLNMDMRLGEGSGAALAFDIIEASNYT
                     YKNMATFDEIDMGR"
     gene            10380..10940
                     /gene="cobU"
                     /locus_tag="ACGI59_001578"
     CDS             10380..10940
                     /gene="cobU"
                     /locus_tag="ACGI59_001578"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HFG9739367.1"
                     /translation="MSKIILVTGGARSGKSNFAEKLCFDRNNNTAYIATSIPFDDEMK
                     DRVKKHQENRPRNWNTYEIYEDIYSIIKEIYKEHETVILDCVTLLVNNLMFEHNLNIE
                     ESSQEEINKLEQYIKEQVVKLIEEIEKTNLYFVFVTNELGMGIVPGNKLSRIYADIVG
                     RINQYIASKSNEVYFVVSGIAMKIKE"
     gene            10950..11723
                     /gene="cobS"
                     /locus_tag="ACGI59_001579"
     CDS             10950..11723
                     /gene="cobS"
                     /locus_tag="ACGI59_001579"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HFG9739368.1"
                     /translation="MKRFILILQFLTRIPIKLNVGFDDEFYKSIVYFPLVGFVIGILS
                     YLIGWISMLLFEPFIASIIITLAGVLITGGLHIDGLGDTFDAIYSYRDKEKMLEIMKD
                     SRLGTNSLLAIMFVLLLKVGFVYDIISNNSLWVIIFMPMIARLGVMLLTYKTVTPREK
                     GMGNLFIGKLTTSMLITAIIYTLLIVALITKFIFLLPNIVLIKVLGSIIVVFVFIILF
                     KKHIYKKIDGVTGDILGCGIELSELVYLIYIYLLIFMFF"
     gene            11743..12357
                     /locus_tag="ACGI59_001580"
     CDS             11743..12357
                     /locus_tag="ACGI59_001580"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFG9739369.1"
                     /translation="MIRLILIRHALTNDNKKGRLSGHINSCISEEGKLQINKITRYLS
                     NENIDKVYTTPSTRTKDTVEKISKLKLLEIEEKEALREISFGDFEGRTFEEIKIKYPN
                     EFEKMIKEGNNYRYPNGESLIDSYKRVAKEIDNIILENNSNLDTKTILICSHAGTIRN
                     IITHLISGSYKYHWNFKIDNASITVLEIDGGFAVIDKMNFTDFI"
     regulatory      12424..12603
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            12718..14232
                     /locus_tag="ACGI59_001581"
     CDS             12718..14232
                     /locus_tag="ACGI59_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HFG9739370.1"
                     /translation="MGKKIMLQGTASNVGKSVLVAGLCRIFKQDGYTVAPFKSQNMAL
                     NSFITKEGLEMGRAQVFQAEAAKIEPIADMNPVLLKPSGNNKSQVIVRGKVVGEMPSS
                     KYHDYKLELVDVLKETFNSLNSRYDIVVMEGAGSNAEINLKDRDISNMGMAEIADAPV
                     IIVGDIDRGGVFASLAGTMLLLNEDEKRRVKGVIINKFRGKKELLQDGVKMLEDIIKV
                     PVIGVVPYTDIKIEDEDSVTTRFKKKMNKSDIHIEIIRTPHMSNFTDFNIFETQEDVS
                     IRYVDYGESLGNPDILIIPGTKSTIDDLTYIRKSGLESQIKNLHSQGKLIFGICGGYQ
                     MLGKKLKDPYHVESEIEEVDGIGLLDTETTFEKEKTTTQVEATIVECISEYMNNLKGK
                     KVKGYEIHMGITKRGNDVRSLDLITKKLDKEVNYTEGSVNKEGNVIGTYLHGIFDEIS
                     FTRAILNNIRKKKGLGELESKVNSFNEFKDKEYDKLADFLREHLDIEKIYEIMK"
     gene            14271..15647
                     /locus_tag="ACGI59_001582"
     CDS             14271..15647
                     /locus_tag="ACGI59_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042242 - cobyrinic acid a,c-diamide
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HFG9739371.1"
                     /translation="MKKILIAGTNSGVGKTTISLGIMQALVKRNMKVQPYKVGPDYID
                     PSYHTFITGRHSRNLDSYMLDDEKIKYIVNKSSKDADISVIEGVMGLYDGFGIDLDNC
                     TSSHTSKVLKSPVILVINGKSMAASSAAMVLGYKELDKDVNIKGVIVNNVKTNSHYQI
                     IKSSIEKYCNVEVLGYFPPNNKFSLESRHLGLVPSVEMKSLREKFYTLADEIEHYIDI
                     DRIIEISESDEFESDFDFQTLIENNKIKEHVNNKSIAIAYDKAFNFYYRENIELLEEL
                     GLEINYFSPLEDVSVPNADYIYIGGGFPEIFGKELEANESMRTSILEAHNNNIPIYAE
                     CGGLMYLGEKLLNQEEQEFNMVGIFSGISKMTSSLKRFGYCIGVAKENTLLSKKGESI
                     KGHEFHHSIFESDEKCVYSMRKERDGNIVEEWDGGYSKGNTLATYLHTHFYNNLNCIE
                     NFLKKGNE"
     gene            15648..16619
                     /gene="cbiB"
                     /locus_tag="ACGI59_001583"
     CDS             15648..16619
                     /gene="cbiB"
                     /locus_tag="ACGI59_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0048472 - threonine-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-phosphate synthase CbiB"
                     /protein_id="HFG9739372.1"
                     /translation="MSILSIYIGYVTDLIVGDPYSFPHPVRFIGKLINFTQGIIRKVS
                     KNDRQLKFGGFILWFITVGITYLTTYAIVKLFSFNILLSIVVNSFIIYTTLATKCLKD
                     EALKIYNVLKTEDIEKSRIQLSYIVGRDTTNLSEPEIIRATVETVAENTVDGIIAPMF
                     YAFIGGAPLAMAYKAINTLDSTVGYKNKKYKDIGFASAKIDDIANYIPARISVILMTI
                     GSFFLNYNYRNCFKISIRDRKNHKSPNCAFSEGAVSGALGIQLGGTNVYFGEKVYKPT
                     IGDKLREIDKEDIIKTNKIMYASSFVSMLVFTLIYVLCRMILINLII"
     gene            16651..17721
                     /locus_tag="ACGI59_001584"
     CDS             16651..17721
                     /locus_tag="ACGI59_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFG9739373.1"
                     /translation="MKDLGHGANVDEMARLYGKNPKEIIDFSSNINPNVLPNLERYIL
                     KGLEECRNYPDINYTNLRENISKYIDINPDFIIPGNGATEVIYLLMKSIKKKLAIINP
                     TFSEYRRSAELNNLDIIDLELDLENNFKLNIDIIKENIKRFDSLFICNPNNPSGNVQD
                     LKELVHLLDKHNKVLIIDETFMEFVEDESKYSLVKYIESNKNIFIIKAVTKFFGMPGL
                     RLGYGLTSNTEIMNKIYEHKEPWTINSFADILSNFIFEDKEYIKNSKEYYIEERKYML
                     QELRNIRNIKVYDTDANFILIRIYKKTTKELKKDLFKQGNILVRDASNFIGLDDSFIR
                     VAIKSHEDNKILIENMKNLLGD"
     gene            17723..18610
                     /locus_tag="ACGI59_001585"
     CDS             17723..18610
                     /locus_tag="ACGI59_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HFG9739374.1"
                     /translation="MKSYGICPASCGEFVQGIIDDEEYLCSYAIDMYSKVYIEEKLVD
                     INLGRYKSRLAIEKVFEKFNLPKKYTKNISLNINSKIPVGKGMASSTADIGATIKATL
                     SLIDKDLSSEEISKLAAEIEPTDSIFIDKNSIFNPLNGTVIKYLGNLTNAKVVILEPN
                     KVLDTMKIRLRQDYNKLKVENKEVIKKSFALLEEGLKKNNLSLVGEACTLSSLANENI
                     EKKEYLNEIIKISKKYGAYGVNIAHSGTVVGILIDKFMNDKKMIDALCESNINSVYNK
                     IYTQNIINGGIKGEIEWNT"
     gene            18598..19230
                     /locus_tag="ACGI59_001586"
     CDS             18598..19230
                     /locus_tag="ACGI59_001586"
                     /EC_number="5.4.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-8 methylmutase"
                     /protein_id="HFG9739375.1"
                     /translation="MEYIKNPMKIEEKSFELIQEIIDEIRPDYKFKNNIEEKIIKRAI
                     HTTADFDYLDILKISEEAVDSIIDALKNNASIYTDTNMALSGINKRKLESLGCKYKCL
                     VADDETVKLAKEKGITRSMAAVEIAAKEEGRKIFVLGNAPTALYKVMEMKSEGRLDLD
                     AVIGVPVGFVGAQESKDEVEKTDIPYIISKGRKGGSNLAAAIVNAILYTM"
     gene            19239..20465
                     /gene="cbiD"
                     /locus_tag="ACGI59_001587"
     CDS             19239..20465
                     /gene="cbiD"
                     /locus_tag="ACGI59_001587"
                     /EC_number="2.1.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-5B (C(1))-methyltransferase
                     CbiD"
                     /protein_id="HFG9739376.1"
                     /translation="MEEYVYIDGKKYRRGYTTGSCATGASKAAVYMLITKNRINTINI
                     DTPKGIPLLLKVDNINISDTFVECSIKKDGGDDIDATHTMDIYARAEIVAKNDKNKGY
                     LTLKDIDNLSTNSECKSELYKFIRVYGGTGIGVVTKKGLSVDVGKPAINPTPLKMINH
                     EIRKLIGDNFESILGNDKVLKITIFAPQGETVAKKTFNPRLGIVGGISIIGTTGIVEP
                     MSDEGWKKSLSIELQMKKEQGLDKIILVPGNHGEQFIREKLNLDIKYVVRVSNFIGYM
                     IKEAQRIGYKKILMAGHIGKFIKVSAGIFNTHSKVADARSEILVANLALMGARYEFLN
                     KINQCVTTEEAVELINNSEYREVYNILSNKCRERVKQYLNEDSDDIDVEVIIFSMDKS
                     LLGKSDNTDDLVEVFI"
     gene            20462..21070
                     /locus_tag="ACGI59_001588"
     CDS             20462..21070
                     /locus_tag="ACGI59_001588"
                     /EC_number="2.1.1.289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-7 (C(5))-methyltransferase"
                     /protein_id="HFG9739377.1"
                     /translation="MINIIGLGPGNLDYITKKGENLISTSDVLIGGKRNLESIKNFEG
                     EKIVLDSNLREIIEYINNNKEKQISIIASGDPLIYGIGRYLSKNIDNKMLNMVSGISS
                     LQYIFSKIYVDMNDVYITSSHGKVPDFDYILSHKKVCMVTDSKIGPKQISREIIDRNL
                     NKIIVVGENLSYDNEKITIAKPEEIIRMDNFDMNVVVILDEE"
     gene            21060..21635
                     /locus_tag="ACGI59_001589"
     CDS             21060..21635
                     /locus_tag="ACGI59_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating cobalt-precorrin-6B
                     (C(15))-methyltransferase"
                     /protein_id="HFG9739378.1"
                     /translation="MRNSEFITGKVPITKEEVRAISISKLNLVNAKNLIDIGAGTGSV
                     SVEAAYNYPNLKVISIEKNDDAIELIEKNKKKFNLKNIEVIKGYAPINLGLNVKVESI
                     FLGGTGNNLEEIIEWSKKNLVTGGRLVANFIIIDTFNQTLKLLKKHGFKEIDVCVLNV
                     SKLEKLGKGEYFKPLNPIYIISCEKGENDDE"
     gene            21628..22380
                     /locus_tag="ACGI59_001590"
     CDS             21628..22380
                     /locus_tag="ACGI59_001590"
                     /EC_number="2.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-4 methyltransferase"
                     /protein_id="HFG9739379.1"
                     /translation="MNKVHFVGAGPGDKELITLKGYKLLSNADVVIYAGSLVNPELLE
                     YCKEDCQIHNSAHMDLQEIIDVMREGIENNKSVVRLQTGDFSIYGSIREQVEDLNKLN
                     IDYDCTPGVSSFLGAASSLGVEYTVPEISQSVIITRMEGRTPVPEKESIQSYAKHQTS
                     MVIFLSVQEIEKVVSKLLEGGYPKDTPIAVIYKATWADEKIVKGTLSDIAVKVKENNI
                     NKTALIMVGRFLGEEYNNSKLYDKDFKHEYRG"
     gene            22364..23485
                     /gene="cbiG"
                     /locus_tag="ACGI59_001591"
     CDS             22364..23485
                     /gene="cbiG"
                     /locus_tag="ACGI59_001591"
                     /EC_number="3.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin 5A hydrolase"
                     /protein_id="HFG9739380.1"
                     /translation="MNIEGNIAIICITENGKKLALKINSLVNNCVIYQVKNKKSNLVV
                     EETCVHTVQKKLSDFVGEIFSKFDYIVFIMATGIVVRSIAPYIISKFSDPAILVMDEK
                     GKNVISLLSGHIGGANSLTQYISSLIDANPVITTATDINQKASLDMIAKKLNAHIDNF
                     RESVKEVNSMLVNNKCVGLYVDEGYQVDTRGFKLIESKDELRDSFSNTYIEKLVVVSN
                     KINCIEDIENLNLDNKFEHCYSTQKIIKVIPRDIVIGIGCRRNTDSELMYNSLEEFLN
                     KQNIDIRAINTVGSIELKKDEKAIIELSKILKVPFKTFSVDEISKVDNLFDKSDFVKK
                     NVGVYSVAEPVAYLLSKGNLLVEKHKYKGITFSVGRIKI"
     gene            23482..24207
                     /gene="cobJ"
                     /locus_tag="ACGI59_001592"
     CDS             23482..24207
                     /gene="cobJ"
                     /locus_tag="ACGI59_001592"
                     /EC_number="2.1.1.131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030789 - precorrin-3B
                     C17-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-3B C(17)-methyltransferase"
                     /protein_id="HFG9739381.1"
                     /translation="MIYVVGIGPGSKDTMTLEAIKAIEDSEAIVGYKTYIKLIEEFIQ
                     GKEIIQNGMRQEVDRCKQAVEEAKKGKKVAVVSSGDAGIYGMAGLILELISKEDEDIK
                     VKVIPGVTASIGAAAILGAPIMHDFCHISLSDLMTPWEVIEKRLRLAAEADFVICLYN
                     PRSKGRSEHLSKAFKIMGEFKGGDTPVGVVKDVGREKEEKFVCTFENMDFEKVDMTTM
                     VIIGNKSTYINGELMITPRGYTV"
     gene            24204..24956
                     /gene="cobK"
                     /locus_tag="ACGI59_001593"
     CDS             24204..24956
                     /gene="cobK"
                     /locus_tag="ACGI59_001593"
                     /EC_number="1.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016994 - precorrin-6A reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6A reductase"
                     /protein_id="HFG9739382.1"
                     /translation="MILILGGTSDSLSICDKINELKNQPYILSVTTDYGRELAQQHAE
                     NVILGKLGKEDMLKFIKDNNIVKIIDATHPYAVEVSKTAITCAKLLNIDYIRFERKSL
                     IEEIDYDNKFIVDTIEEACEIANKIGTNIFIGTGSKNLNRFIEEISDKNLVVRVLPTS
                     EVILSCEKLGLSADNIVAMKGPFSQYINEETYKHYNIDLVITKESGIAGGFLEKVNAC
                     KTFNIPVIIIKRESINYENVFNDINKIKDILV"
     gene            24975..26453
                     /locus_tag="ACGI59_001594"
     CDS             24975..26453
                     /locus_tag="ACGI59_001594"
                     /EC_number="2.1.1.151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-factor II C(20)-methyltransferase"
                     /protein_id="HFG9739383.1"
                     /translation="MKKALLVVSFGTSYHETREKTIDICEDKIKNSLKSHDFFRAYTS
                     NMIINKIKKRDGIEIDNPIQALDKIYEQGYDEVIIQTLHIICGEEFNKLKEQVDGYSS
                     KFKKIVLGRPLLTHIEDYQEAVEAIKHQIPHMESNEAVVFMGHGTLHESHSAYPALEY
                     MLRDNGINAYVGTVEGYPEIEHVIRRLKEGNIKTVNLMPFMLVAGDHAINDMAGDEED
                     SWKTILEENGFNVRVHLKGLGENSYIQDKFVRHAVKCAENAKFYGIGAGPGDGSLLTI
                     KAVNTLKKLDILYTPESKKGGDSLALSIVSEYLPESVEIKSRHFPMSFDGNEKSLAWD
                     NVAQEIISDVNDGKNVGFVTLGDPMIYSTYVYIMKRILGEVEVETIPGISSFSNIASN
                     QNFPLVMDKEALVVIPCTMEDDKIEYALQNYNSIVLMKVYKNFKEIIKKLEKYDLIEH
                     AILVSNSSQESENVFTDLKEAHLEDKISYFSTILVNKDNKIK"
     gene            26483..27388
                     /gene="hemC"
                     /locus_tag="ACGI59_001595"
     CDS             26483..27388
                     /gene="hemC"
                     /locus_tag="ACGI59_001595"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HFG9739384.1"
                     /translation="MNIVVGTRGSNLALIQTEWVINELKKKYPEISFEIKIIKTKGDL
                     IQNVSLDKIGDKGLFVKEIEQQLLDGKIDIAVHSMKDMPSYLADGLKFAHTPKREDPR
                     DVLILREGYKNLDDLPHGAVIGTGSKRRKFQLLKQRPDLNIVQVRGNVETRIRKIKDE
                     NMHGIVLAASGIIRANLQDKISSYLPVDVVIPAPAQGALAIEIRSNDSTIEGIVNSLK
                     DENTEIQILAERGFLDGVNGSCHIPMAAYCEIIQDKIHLTGLYGDSEGKKVVIKSIDG
                     DISSPRELGLKLAKLVLKEYENYEG"
     gene            27378..28877
                     /gene="cobA"
                     /locus_tag="ACGI59_001596"
     CDS             27378..28877
                     /gene="cobA"
                     /locus_tag="ACGI59_001596"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HFG9739385.1"
                     /translation="MKGKVYLVGAGPGDYKLMTLKGLECIRKSDVIVYDRLANSSYLR
                     EAKPDCELIYVGKASSNHILPQEDINRIIAEKAKEGKIVTRLKGGDPYVFGRGGEEAE
                     TLVDEGIEFEVVPGITSAIGGLCYAGIPITHRDHASSFHVITGHLKGDDSGELNWNAL
                     ANNKGTLVFLMGISNLEKISENLMKEGKDKDTPVALISWATRYNQRVVTSTLENVYET
                     AIKEEVKPPTLIVVGSVVGLREKLNFFESKPLFGKSIAVTRSRNQNSVLVEKIMDLGG
                     NPIEIPTIKVEKIQNNINLENEIKNINKYNYLILTSKNAVEIFFEKIYEMNFDLRILS
                     NLKVCAIGSATSNELKKRGIIADIVPKKFVAESLYEELAPILNDDDNILIPRAENARD
                     YLVDKLKAICEVTEVHIYRTVIDEEKKGEILDILNSNDIDYITFTSSSTVNNLVEIIG
                     KENIKILEKIKTLSIGPITTETINSLGIKLYKESATSTIDSLVDLIIKN"
     gene            28894..29859
                     /gene="hemB"
                     /locus_tag="ACGI59_001597"
     CDS             28894..29859
                     /gene="hemB"
                     /locus_tag="ACGI59_001597"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HFG9739386.1"
                     /translation="MLRRPRRLRASKAIRNLVRETKVNVEDLIYPLFVVEGENIKEEI
                     KSLPDVYHLSIDMLEDEIKEISELGIEHIMLFGVPDEKDECASQAFNDNGIVQKAIRK
                     VKEINSDINVITDVCMCQYTSHGHCGILTDTGYVDNDKSLEYLAKISVSHAKAGADMV
                     APSDMMDGRIQAIREALDENGLENVGIMAYSVKYASNYYGPFREAAHSAPSFGDRKTY
                     QMDPANSNEAIIETELDILEGADILMVKPALSYLDIIRRVKDNFDLPLAAYNVSGEYS
                     MLKFAVKEGLLNEDVILESITSIKRAGADIIITYFAKDIAKLLKK"
     gene            30012..30590
                     /locus_tag="ACGI59_001598"
     CDS             30012..30590
                     /locus_tag="ACGI59_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HFG9739387.1"
                     /translation="MLYPINLKLDELDVVIIGGGEVAYRKCKNFLEFNKNVTIVSKQI
                     LNKFYDLKGNIKIIKDDYKEIYIKDASVIIAATNNRELNMEIGLYCKAKNKLVNVVDN
                     IEISNFTVPSYIKRGDLLISVSTGGKSPSLSSKIKKEIEERYTEDYEEYLNVLGDIRK
                     KVIKKYEDKSKRKDVLNMLVTLDLEELKKFDI"
BASE COUNT        12422 a         3066 c         5675 g         9517 t
ORIGIN      
        1 aggatataaa attcttaaat aaaaagaaaa ggttgttttt ataaaaaata ttttttatac
       61 cactttaaaa aacacaataa taacactgag ggaggtgggg atatgagaga acgcattatt
      121 cttgaaacat taaaaaatag tagtggttat ctaaacatag attattttgc agataagttg
      181 ggggtatcta ctcgaactat cagaaatgaa ataaaaaaga tagaaactat aaaagaatgt
      241 aatggtttta aattagaata taagtctaaa ttaggatata ttctaaatat taaagaccaa
      301 gataaattcg agcattatct aagaaactta ccctcagatt tagttgaaaa tccagaacaa
      361 cggttggagt ctataatagt agagctttta gtaaatgaag gttataaaac tatagaacag
      421 ttaagtaaaa aatttctagt aagttcatcc caaataaaaa atgatttaaa aaagatagat
      481 gaaaagataa aggatacaga attaaaacta gagcgaaagg ctcattatgg aattaaaata
      541 gaaggttcta taaaagaaat tcaaagtata ttggtggata gttattttag agggaataga
      601 aatataacag agtacaggaa taagtttata gataatttaa aattagctaa tattagaagt
      661 acaataaaaa atgtacttaa tgaacatgat ttagaagcaa atttaacaga acttgaggaa
      721 atattagcac aaattattat attgtatata agagttagta tgagagtagt gggaaatgct
      781 agcgatttaa aattatatac agaagattta attatagata aattattaga taaaattttt
      841 agagataaaa aatataacct taattatgat gaaaagtatt atttaaagga gcttataaaa
      901 ctaaagacta aagataagaa agccactata aagaatattg ataagaataa acttcaagat
      961 attatgttcg aattttttag agagatagat aaaaaatata actctaattt tttagaagat
     1021 aaagagtttt ttaacttatt ttacttacat attgcatgtt taatagaacg tataaagaag
     1081 aaccacaaga taataaatcc tttttcagtt aaaataagtc aacagtatcc aactgttttt
     1141 aatttatcta ttcaattttc aaaaatcata gaaaatgagt atcatataaa aataagtcaa
     1201 gatgaaatag ggtttatagc tactcacatt gctgttcctt ttgaaaagag agaagaagct
     1261 aattttaata aaaaatataa aatagcaata atatgctctt ctggtggagg tagtgcattt
     1321 ttgataaatt taagacttaa agaaattttt ccaaatgctg agataagaaa tttttcatta
     1381 ctagaagaaa aagctgtaat agaatttgca ccagatttga ttttttcaat aacaaatctt
     1441 ttatttgaaa taaatgctcc agttatactt ataaatgaaa ttttagacga attggattat
     1501 ttagatatta aagaaagtgt tagatttgca gaccatatag gaaatatatc aagcccaaaa
     1561 caatatatat taggtctatt tgataagaat cactttaggt gtataaaaga taaagctaag
     1621 tataaggata tccttgatag tatgtcaaaa tgtatagttg acgagggagc ttgttctcca
     1681 acctatccca aagatgtatg ggaaagagaa agttatttga gtactattta tacaaatggg
     1741 gtggctatac ctcatccaat cgagatgaca ggaaacaaaa atataatatc agtagctttg
     1801 atacaaactg atattattta tgaaaatagg gtgcctaaaa ttattttcat gatttcatta
     1861 ataaagggaa atttagaact tcataagcaa atatctaaat atttaagtaa gattatggca
     1921 aataaggata tggtagacat gctaaataag tcacaatctt atgaagagtt tatgtataag
     1981 ttaaaaatct atctgggagg ttagacatga atatagagtt ttttaagagg ttgtcagaag
     2041 cagatggtat agcatctaat gaagaagagg ttagacatgt tttattggaa gaactaaaag
     2101 cttatagtga taaaattatc tgtgatgggc tagggagtat catcttttct aaaataaaag
     2161 atgagagtgc accaaacgta atgatttgtg ctcatatgga tgaagttgga tttatggtga
     2221 gaagtataga caaacttggt atgattcact taataacaat aggaggagta aaacctcttg
     2281 cacagtttgt acaaaaagtt agaattacaa caaaagaagg gaagaaaatc cccgctgtca
     2341 taaatgctac atataataat ggtaaggctg aaaatatata tgcagatatt ggagcatata
     2401 cagaagaaga tgtatataac ctagggatta atgttgggga tatggtaaca tatacaacaa
     2461 gctttgagga atttacttta ccagaccgat tagtgggaaa ggcttttgat gataggatag
     2521 gatgttttgt gatgggagaa gttttaaaag aacttaggaa agaaaatcta aattgtaata
     2581 ttcactttgc ggctacaagt agtgaagagg ttggaataag gggagcaaag acatcaacac
     2641 agttaataaa tccagatata gtatttgtaa tagatgtagc atgtgctaaa aatgagtttg
     2701 tgagagacta tacaaataag aaacaaattg ataaaggtgt aatgctaatg catagagata
     2761 ggactctagt ccctagtaga aaaatgatag attatgtaat ggaaattgct gataaaaata
     2821 atataccact tcaacatgat atgtttgaat caggtgggac tgatggagga gaagcacatc
     2881 tcgtaaatga agggaaacct tgtgtagtaa cttgtgtacc agttagatat ggtcattgtg
     2941 cattttcaat agtaagtaat aaagatttgg agaacattat aagattgtat acacagttaa
     3001 ttttaaattt tgatgaagaa caatataaac atataagaaa ttttatctaa aggagaataa
     3061 gaaaatgtca acagaagtac aaataatgga acttatatca actgcaggag agagtaaatc
     3121 gaaagctttt gaggctttaa aaaaagtgaa gtcaaaagat tttaaaggag caaaattgtt
     3181 aatagaggag gctcgtaaaa tagatattga agctcacagg atacaaacag aattaataac
     3241 tcaagattta aatgaagatg ttgaaagtcc agcaatgagt cttttaatgg tacatgctca
     3301 agaccactat atgacttctc agctagcaaa agacttgata gaggctttga tatattgtct
     3361 tgataagtaa ttgtaatata gaaatagatt atttatgtaa aaattctttt aaaagtttag
     3421 atgcttttaa gagataataa aaatacaaac tttaggagga attattatga aaatattatt
     3481 atgttgtgca ggtggatttt ctactaatat gttaatgcaa aatatgaaaa aggtggtaaa
     3541 agagagtgct aaactcaatg aaaaagattt tgagtttaca gctataccag cagattcaat
     3601 agaaagtgaa gttgatgatt gggatatagt acttataggt cctcaaatag ctcacaaaat
     3661 agacttctta aaaggaatat tagagccaaa aaataagcct tttgcagtaa tagataaaga
     3721 tgtatatgga aaaatggatg gagctacagt tatgaagttg gcattaataa cttataagaa
     3781 acatcaatta gctcaaggat aataaaaata atgagggaaa agggagatta gatatgtttt
     3841 caaaatttga agcatttatg aatagagtat ttatgccttt agctcataag gttgacaatc
     3901 aacgtcacct tggttctata aaggctggta tggttgctat gacaccattc acaatattag
     3961 gaagtatatt tgcaatactt ccagcacttc caaatatgct tggagaaaat aatccaataa
     4021 gcaaattcat acttagcaat tcagcaatat tggatttacc agtaagtctt tccataggtt
     4081 taataggatt atatgcttgt atgtgtattg cttttaatct ggggaactac tataaattat
     4141 atataccagg ttgtgttgca ctatcaactt tttctttttt attcctagta gcctcattta
     4201 caccagatgg agctttagaa ataggaaatt taggagctaa agggctattt acaggtatga
     4261 tagcagggct tggttctgtg gagttatata atttttgtaa taagaagaat atcacaataa
     4321 aaatgccaga aaatgtacct gattttgtat ctaggtcttt tgaattaata cctactacta
     4381 taatagtttg tggaactttt atagttgctc gttttatttc attaaaagta tttggagaat
     4441 tacctccaca gatacttact caatttttat ctccattagt aggaagtatg gataacccat
     4501 ggtcagttct tgcacttaac tttgctatat gtactatatt cttttttgga atacattcat
     4561 cagtatttag tcctataact agacctataa tgattacatt tatagctgaa aacatagcag
     4621 ctatgcaggc tggagaaaca atacctcatt tctttacagc aggagcttca agtgcattct
     4681 ttggatttac aggatgtgga attagtatag gttgtgtaat tgcatgtatg ttatcaaaaa
     4741 atgcttccta taagaagatt ggacgtgtat cattattccc tgcattattt gggataaatg
     4801 aaccaatatt atttggagca ccgataatat taaatccaat aatgtttata ccacatgtat
     4861 ttggtggagc tataataggt acactaccaa tgttttttat gcactgggga tggttagcaa
     4921 agccagtatt tgaccctcca tatgtagggg tattcttaga aggattttta actaatggag
     4981 attggcattc tatattagca aatgcattgc aattagtatt agcagtttta ttatattggc
     5041 cattctttaa aataatggaa cgtcaacaaa ataataaaaa acaaaaagaa gaaactaaga
     5101 atatattctc taaagaagaa gaaggcttat tggatgattt aggattggat ttctaatatt
     5161 ttgttatgaa taatacaatg gagagattag tatccttaat ctataaaaga ggtgttgacg
     5221 ctcttttgat taaagcaaaa aatactaaaa gatatatagg agctttatca ggtagtggtg
     5281 tatatgtgct tgttactaag gataaaaatt atcaaattct agatggcaga tatattgatg
     5341 aagcagataa aaaaacgact ggtttcataa aaaggattgt atctcaaggt tcttatatac
     5401 caactataat tgagttatta aaagaattaa atataaaaaa gttagcaata gaaaaccaag
     5461 ctatgagtat acaagagtat ctttcactaa aaaatgaagg ttttgaaatc aatttaataa
     5521 caaatgaatt atggaagcta agggctataa aatctaaaga agaaattaaa cttattaaaa
     5581 aggcttgtga gattacagat gaagtatttg atgaggttat ttctgaaatt aaaaaagata
     5641 tgactgagct agaagtatca gctctaattc agtatcatgc attaaaaaaa ggtgcaagtg
     5701 gaatgtcttt tgaaacaata gtggtatcag gagaaagagg tgcaatgcca catggaagac
     5761 ctacaaacaa aaaattgaaa gtgaatgaag ctataacaat agattttggt gttgtttatc
     5821 aagggtatca atcagacatg actcgtacaa tatctatagg caaacctcct aaaattatta
     5881 aagagatata tgatgtagta ttggaagcac aattatctgc aatagagtca ataaaggaag
     5941 gaactagggc aagtgatgtt gacaaagtgg ctagaaaaat tatagataaa catggatttg
     6001 gcgaatattt taatcatgga ctgggtcatg gtataggtct tggagatggt gaagttccca
     6061 ctttaaatcc aaatagtgaa gatatattag tggaaggaat ggtaatgtct tgtgagccag
     6121 gtatttatat tccaaatgta gggggagtta gaatagaaga tgatattgtt ataatagatg
     6181 gaaaaggtgt tccactcaat aagacatcca aagagtttat aatattagga gagtaaaatt
     6241 atgaaatata attttaataa agtattaaat cgtaaagaag gaaattgtag aaaatggagc
     6301 aaccatgtga ttaaagaaaa atttggctta aatgaagatg caattcctat ggatttagct
     6361 gatatcgatt ttgagtgtgc tcctgctata aaagaatcta tcttaaaaag agcatcagta
     6421 ggagattata gttatacttt tataggagat gacttctatg atgctattat aaattggaat
     6481 aaaagaagat ttgatgtata tatagaaaag gattggataa aattaacttt tggtactgtt
     6541 tcaacccttc attatatagt ccaagcatat actaaagaag gagagggtgt attgataaat
     6601 acacctgctt atgacccttt tgcagaggct gtagtaaata ataatagaaa gttgtgttgt
     6661 agctcattaa aattagataa caatagatat tatttagatt tagaggatat ggaaaatcaa
     6721 atgaaatacg agaatataag attgcttata ttctgtagtc cacaaaatcc atcaggtcgt
     6781 atatggacaa aagaagaact atatcaagtg tcagagttat gtttaaagta taatgtaatt
     6841 ttggtaagtg atgaaattca tagagatata atatttaaag attatagatt tgtatctttg
     6901 tggaatgctc atccagaaat ttataaaaat agtataatct gtgtatctcc taataaaggc
     6961 ttcaacttag gtggattaaa aacatcatat gtattaataa gagatgtaaa aattagggag
     7021 actttattag aaaggctaaa aagtaattca ataacttctc caaatgtatt tgcaatacca
     7081 gcaatagttg cagcatataa tgaaagcgaa gaatggctag atgctatgac tagttatgta
     7141 gaggagaatt ttgaaatagt ttatgatttc tttgaaacta atataccaaa agctaaagtg
     7201 atgaagtcag actcttcttt tttagcctgg atagatgtaa gagaagtatt tagaaatgaa
     7261 gaagaatcta aagaattttt tagacatgca aacttaacta tggtagttgg aagttatttt
     7321 gtgaaagatg gagatggatt tattcgtatt aatgtggggt gtcctagaga aacattaaat
     7381 gaggctctaa atagaatcaa aaaaacgtat ataagtatgt attgttaaat cataaaaaaa
     7441 gtatctatgg tttatataaa aacacattat ttagttatca aatattactg gaattttgta
     7501 ttccagtaat attttttgat taaggaatat tattgaggaa tattaaataa tattatttca
     7561 tacccttata ttaacttata taaaaatatt ttattatttt gggaacgggt tagctaacct
     7621 ttatctagtg tatatttata ttgtaagaag cagtaaaaac ttttccttta ctaagtttat
     7681 taataaactt cttatcttta taaattttgt tagaatttat actatcagca agaccatacc
     7741 aaggttctat acataaaaaa ggagcagtag aattagtttc actataataa ggagtccaaa
     7801 taccaactag agggaaatct tccatagata tagttacata tttactacca tcattattac
     7861 atatagacac ttcatctatg ttggtgtata tcaaagcatc attcttgaat acttctggat
     7921 ttaactgtaa gttttttaat tttccaatag gtattatatc atctgtaaaa gaaccattta
     7981 aattgatttt ttctacatca tttttacttt taaactcaag gtagtgttta gaaaaatcat
     8041 tttgttcgta aaaaggaagg ttgaatgcag gatgtccacc tatagaaaag aatatatctt
     8101 tagaatcagt attttttaca atccattcta cctcaatact attatcattt aatgtatagt
     8161 ttatgattag ctcaaaagaa tatggatact tctttaaagt agattcatta tctgttaatt
     8221 tataagttac aaatgtatct cctttatcta caatctcaaa gtccatatct ctagcaaacc
     8281 catgttgagt catgctatat aaattttctt ctattattgt ttcattatca aaaagtttac
     8341 ctactatagg gaacaatata ggtgattgtc taccccaata ttttttatta ccatcccaaa
     8401 gaatgttttt gtcgtatttt ttagaaaata ttttagttag ttcagcacca aaactgcttg
     8461 attcgataat taaattttca ttttgtaata tattcattta aaatctcctt tattatattt
     8521 tatgagtata ttatactata aagtataaag attattaaat tttatttgat attatatttg
     8581 aaaggtagat tttagtatat tttgaaaatt ttgttttgaa aatttgggat tatttaagaa
     8641 attttgtatt ataaatagaa ggaaatagat aaattgtata gaataaaata tattaagata
     8701 agttaataat gttaaatggg aagtgagttt atggaaaaac caaatacaaa agttgttaac
     8761 tttcaagagt ataaagacag aaaaaaccaa agagaagtaa atagagacga acttttaaag
     8821 cttataaaaa aaattgtgac ttcaaaatag ggagttgtta aactccctat tttaatgggt
     8881 ttcataataa ttaagacatt ctaagtccat ctatataatc tttgaattgt gagaaatctg
     8941 attcactact taattctatg gtttctgaac cttccgtata aaaattctta agatagatag
     9001 tataattatt atcaacacaa tcatgataaa tacatctata tccatcttca tacaaacttt
     9061 ttaacttaga aaaatcatcc acaatatcat ctccttaaag ttcttttaaa tatattttta
     9121 caaaaagcat ctaattgtat gcaaattgac aatgtaaaat aaaggaaatg aacataaaat
     9181 gtagaataat ttttatatgt atgtaaaaat aaaaagaaaa agttgaaatt tttagaaaat
     9241 aagagtattt tttatctaaa aagagagttt aaaaaataaa aaaatgttgg gaggataaaa
     9301 aatgagttta ttagaaagta tatcaaaaaa catatattcc ctagataact cttctattga
     9361 aaaaacaaag cagagattgg ataggcttat acatccaact ggaagcttag gaaagataga
     9421 agatatttgc atgcaattag caggaatatt tggcaatgaa aattttgaca caagtaaaaa
     9481 ggttataatt gcttttgcgg gagaccatgg tgtatatgaa gaaggcgtag cacctgaccc
     9541 tcaaaatata acaaaattac agtttccaaa tttttcgaaa ggtctatgcg gagttggagt
     9601 aataagtaaa tttgtagggg cagatgttgt agctgtagat gtaggaataa actgtgatga
     9661 aaaactggat ggggtattag actacaagat aagaaaaggg acatcaaata tggcaaaagg
     9721 tccagctatg agtaaacaag aagcaattag atgtttagag ataggtattg aaatagcaga
     9781 gcagtgtata gaaaaagatt ataaggttat tggtattggg gagatgggta tagcaaatac
     9841 tactccaagt acagcaataa tatctgtaat ttcagggtgt gacccgttag aagttactgg
     9901 tataggtgca ggtcttaaga aagaaagact aaagcataag gctgaagtaa ttagaaaggc
     9961 tattgaaatt aataatccaa atccaactga tggagtagat attttatcta aagtaggagg
    10021 gtttgaaata ggctcaatgg caggtgttat attaggttgt agtgctaata ggattccagt
    10081 agtaattgat gggtttattt cttatgcagc agcattaatt gcatataaga taaatccaaa
    10141 aactagagaa tatatgatag catctcattt atcagcagaa tcaggaacta aaagagcatt
    10201 agacatatta aaattagacc ctctattaaa tatggatatg agattaggtg agggaagtgg
    10261 agctgcttta gcatttgata taatagaagc ttcaaactat acttataaaa atatggcaac
    10321 ttttgatgaa attgacatgg gaagataaat ataaaaattt aatatgtagg agtgttttga
    10381 tgagtaaaat tattctagta acaggaggag ctaggtcagg gaagagtaac tttgcagaaa
    10441 aactatgttt tgacagaaat aataatacag cttatatagc aacctctatt ccatttgatg
    10501 atgaaatgaa agatagagta aaaaaacatc aagagaatag acctagaaac tggaatactt
    10561 atgaaatata tgaagatata tattccataa ttaaggagat atataaagaa catgaaactg
    10621 ttatactaga ttgtgtaaca ttattagtaa ataatctaat gtttgaacac aatctaaata
    10681 tagaagaatc tagtcaagaa gaaataaaca aattagaaca atacataaaa gaacaagttg
    10741 tcaaacttat tgaagaaata gaaaaaacaa atctatactt tgtatttgta acgaatgagt
    10801 taggtatggg aattgttcct gggaataaac ttagtagaat ttatgctgat attgtaggtc
    10861 gtataaatca atatatagct tcgaaaagta atgaagttta ttttgtagtt agtggcatag
    10921 ctatgaagat aaaggagtag tatgcaatta tgaaaagatt tattttaata ctacagtttt
    10981 tgacaaggat acctataaaa ttaaatgtag gatttgatga tgaattttat aagtcaatag
    11041 tatattttcc acttgtaggt tttgtcatag gtatattatc ctatttaata ggatggattt
    11101 ctatgctttt atttgaacca tttatagctt ctataataat aactctagct ggtgtcttaa
    11161 ttactggagg acttcatata gatggattag gagatacatt tgatgcaata tatagttata
    11221 gggataaaga aaagatgctt gaaataatga aagattcaag attaggtaca aattcacttc
    11281 tagcaattat gtttgtactt ttattaaagg ttggattcgt ttacgatata ataagtaata
    11341 attcattgtg ggttataatt tttatgccaa tgattgcaag gttaggagta atgttattga
    11401 catataaaac tgtgacacca agagaaaaag gaatgggaaa tttatttata ggaaaattaa
    11461 ctacaagtat gttaataact gctataatat acacattatt gatagttgct cttattacta
    11521 aatttatatt tttattacct aatatagtgt tgattaaagt attaggttcg attatagtgg
    11581 tttttgtatt tataatatta ttcaaaaaac atatttataa aaaaattgac ggagtaacag
    11641 gagatatatt aggctgtgga atagaacttt cagagcttgt atatttaatt tacatatatt
    11701 tgttaatttt tatgtttttt taaagtaaaa agtggtgata atatgataag attaatatta
    11761 ataagacatg cactgacaaa tgataataaa aaaggaagat tatcaggcca tataaatagt
    11821 tgtattagtg aagagggcaa actacaaata aataaaatta ccagatatct gagtaatgaa
    11881 aacatagata aggtttatac aacaccatct acacgtacta aagatacagt agaaaaaata
    11941 tcaaaattga aattattaga aatagaagaa aaagaagctt taagagaaat tagttttgga
    12001 gactttgaag gaagaacttt tgaagaaatt aaaatcaaat atccaaatga gtttgaaaaa
    12061 atgataaaag aaggtaacaa ctatagatat ccaaatggag aaagtttgat agactcttat
    12121 aaaagagtgg caaaagaaat tgataatata attttagaaa ataatagtaa tttagataca
    12181 aagacaatac ttatatgttc tcatgcagga acaataagaa atataataac acatttgatt
    12241 tctggtagtt ataagtatca ctggaacttt aaaattgata atgcatctat tacagtttta
    12301 gaaattgatg gtggatttgc tgtaatagat aaaatgaatt ttacagactt catataattt
    12361 aatcaaaacc aaacattaca atctgtgttt gtaatatatc tgtaaaatta gcaatgaaaa
    12421 tagaatatta gattaaaatt aggttcttaa ttagatgtta aaagggaaaa aggttagatg
    12481 cctttgcagc ccccgctact gtgaaaccaa cgaaactata aataccactg tcagtttgat
    12541 gggaaggtta tagaagtagg atgaagttaa gccaggatac ctgcctaatt taatttaaaa
    12601 caagaggtct tcggggtgaa agatttttaa ttaatatata taaaagtcct atctttagaa
    12661 gtattaggac tttttttaat ttgtttttta gtaaatttta acaaggaggg atggaaagtg
    12721 gggaaaaaaa taatgctaca aggaacagct tcaaatgtag gtaaaagtgt cttagtagca
    12781 ggtttgtgta gaatatttaa gcaagatggt tatactgttg ctccttttaa gtcacaaaat
    12841 atggctttaa attcttttat aactaaagaa ggattagaaa tgggaagagc acaagtattt
    12901 caagcagagg ctgcgaaaat agagccaata gctgatatga atccagtttt attaaaacct
    12961 tctggaaata ataaaagtca agttattgta agaggtaaag ttgtaggaga aatgccttct
    13021 tctaagtatc atgattataa attggagtta gtagatgtgt tgaaggaaac atttaatagc
    13081 ttaaattcaa ggtatgatat tgtagttatg gaaggggcag gaagtaatgc agagataaac
    13141 ttaaaagata gagatatctc taatatgggt atggcagaaa tagctgatgc acctgtaata
    13201 atagtcggag atattgatag aggaggcgta tttgcctcat tagcaggaac aatgctttta
    13261 ttaaatgaag atgagaaaag aagagtaaaa ggtgtaataa tcaataaatt cagaggaaaa
    13321 aaagagcttt tacaagatgg tgtaaagatg ctagaagaca tcataaaagt acctgttata
    13381 ggagtagtac cttatacaga tataaaaata gaagatgaag acagtgtaac tacgagattt
    13441 aagaaaaaaa tgaataaaag tgatatacac atagaaataa ttagaacccc acatatgtca
    13501 aattttacag attttaatat atttgagaca caagaagatg taagtataag atatgtagac
    13561 tatggagaat ctttaggaaa tccagatata ttaataatac ctggaactaa aagtactata
    13621 gacgatttaa catatataag aaaaagtggt cttgaatcgc aaatcaaaaa tttacatagt
    13681 caaggtaaat tgatatttgg gatatgtggt ggataccaaa tgttaggtaa aaaattaaaa
    13741 gacccttatc atgttgaaag tgaaattgaa gaagttgatg gaataggtct tttagataca
    13801 gaaacaacgt ttgagaaaga aaagactaca acacaagtag aagctactat tgttgaatgt
    13861 atcagtgaat acatgaataa ccttaaggga aaaaaagtaa aaggttatga aattcatatg
    13921 ggaattacaa agcgaggtaa tgatgtaaga agtttagatt taataactaa aaaacttgat
    13981 aaagaagtga attatactga gggaagtgta aataaagagg ggaatgtaat tgggacttat
    14041 cttcatggaa tatttgatga aataagcttt acaagagcaa ttttaaacaa tataagaaag
    14101 aaaaaaggtt taggagaatt agaaagcaaa gtaaactctt ttaatgaatt taaggataaa
    14161 gagtatgata agttggctga ttttttaaga gaacatttag atatagagaa aatatatgag
    14221 attatgaaat aatgatgacc tttatttata atttaggtag gaggtgtatt atgaaaaaaa
    14281 ttttaattgc gggtactaat agtggagtag gtaaaacaac aatatcatta ggaataatgc
    14341 aagctttggt taagagaaat atgaaagtac agccatataa agtagggcca gactatatag
    14401 acccttcata tcacactttt ataactggaa gacattctag aaatttagat tcttatatgt
    14461 tagatgatga aaaaataaag tatatagtaa ataagtcctc aaaagatgca gatatttcag
    14521 taatagaagg tgttatgggc ttatatgatg gctttggaat agatttagat aattgtacaa
    14581 gttcacatac ttcaaaggta ttaaagtcac ctgttatatt agttataaat ggtaaatcta
    14641 tggcagcatc tagtgctgct atggtacttg gatataagga actagataaa gatgtaaata
    14701 taaagggtgt tatagtaaat aatgtaaaga ctaatagcca ttatcaaata attaagagtt
    14761 ctatagaaaa atattgtaat gttgaagtat tggggtattt tcctccaaat aacaaattct
    14821 ctttggaatc tcgacatcta ggattagttc ctagtgtaga aatgaaatca ctaagggaaa
    14881 aattttatac cttagcagat gaaattgaac actatataga tattgacaga ataattgaaa
    14941 tatcagaaag tgatgaattt gagagtgatt ttgattttca aactttgata gaaaataata
    15001 aaataaaaga acatgttaat aataaatcaa tagctatagc atatgataaa gcattcaatt
    15061 tttattatag agaaaatata gagcttttag aggaacttgg tttagaaata aactatttta
    15121 gccctcttga agatgtatca gtgccaaatg cagattatat ttatattgga ggaggttttc
    15181 cagaaatatt tggaaaagaa ttagaagcaa atgagtcaat gagaacttcc atattagaag
    15241 cacataacaa taatatacct atttatgcag aatgtggagg actgatgtac ctaggtgaga
    15301 aactattgaa tcaagaagaa caagaattta atatggttgg aatatttagt ggaattagta
    15361 agatgacatc ttctttaaag agatttgggt actgtattgg tgtagccaag gaaaatactc
    15421 ttttatctaa aaaaggggaa tccattaaag ggcatgagtt tcatcattca atatttgaaa
    15481 gtgatgaaaa atgtgtatat tcaatgagaa aagaaagaga tggaaatata gtagaagagt
    15541 gggatggtgg ttatagtaaa ggaaacactt tggctacata tcttcatact catttttata
    15601 ataacttaaa ttgtatagaa aattttttga aaaaaggaaa tgaataaatg agtattttat
    15661 ctatttatat aggatatgtg acagacttaa ttgtaggaga cccatattct tttcctcatc
    15721 cagtgagatt tattggtaaa cttataaatt ttactcaagg tataataaga aaggtctcta
    15781 aaaatgatag gcagttaaaa tttgggggat ttatattatg gtttataacc gttggaataa
    15841 catatttaac aacttatgct atagtgaagt tatttagttt taatatatta ttgagtattg
    15901 tagtgaatag ttttataatt tatacaacac tagccacaaa atgtttaaaa gacgaagcat
    15961 taaaaattta taatgtatta aaaactgaag atatagaaaa atctaggatt cagctatctt
    16021 atatagtagg tagggatact actaatttaa gtgaacctga aataataaga gcaactgtag
    16081 aaactgttgc agaaaatact gtagatggta taattgcgcc tatgttttat gcatttattg
    16141 gaggagcacc acttgctatg gcctataaag ctatcaatac attggactca acagttggat
    16201 ataaaaataa aaagtataaa gatattggtt ttgcatctgc taagattgat gatatagcca
    16261 attatatacc agctagaata tctgtcattc ttatgacaat tggaagtttc tttttaaatt
    16321 ataactatag gaactgtttt aaaatctcta ttagagatag aaagaatcat aaaagtccaa
    16381 attgtgcttt ttcagaaggt gcagtatctg gtgctctagg aatacaacta ggaggtacca
    16441 acgtttattt tggggaaaaa gtatataagc caacaatagg ggataagctt agggagatag
    16501 ataaagaaga tataattaaa actaataaaa ttatgtatgc atcttcattt gtatctatgt
    16561 tggtatttac actaatatat gtgttatgta ggatgatttt aattaactta attatataaa
    16621 ttattgtttg ttaacttaag ggggacatag atgaaggatt tagggcatgg tgccaatgta
    16681 gatgaaatgg caagattata tgggaaaaac ccaaaagaaa taattgattt tagttcaaac
    16741 ataaatccaa atgtattacc aaatttagag agatacattt taaaaggatt agaagagtgt
    16801 aggaattatc cagatataaa ttatacaaac ttgagagaaa atatatcaaa atatatagat
    16861 ataaatccag actttataat acctggcaat ggtgcaacag aagtgattta tttgcttatg
    16921 aagagtatta aaaaaaaatt ggctataata aatccgactt tttcagagta tagaagaagt
    16981 gctgagttaa ataatttaga tattatagat ttagagttag atttagaaaa taactttaaa
    17041 cttaatatag atataataaa agaaaatata aaaagatttg atagtttatt tatatgtaat
    17101 cctaataatc cgagtggtaa tgtccaagat ttaaaggaac ttgttcattt actagataaa
    17161 cataataagg tactaataat tgatgaaact ttcatggaat ttgttgaaga tgaaagtaaa
    17221 tatagtttgg ttaaatatat agaatcaaat aaaaatatat ttataattaa agctgtgact
    17281 aagttttttg gtatgcctgg tttaaggcta ggatatggtc ttacaagcaa cactgaaatt
    17341 atgaacaaaa tttatgaaca caaagagcca tggactatca attcttttgc agatatacta
    17401 tccaatttta tttttgaaga taaagagtat attaaaaata gtaaagaata ttacatagaa
    17461 gaaagaaaat atatgttaca agagttgaga aacataagaa atataaaggt ctatgataca
    17521 gatgctaatt ttatcttgat aagaatatat aaaaaaacaa ctaaagaatt gaaaaaagat
    17581 ttgtttaagc aaggaaacat attagttaga gatgcatcta attttatagg attagatgat
    17641 agctttataa gagtggctat aaaatctcat gaagataaca aaattcttat agaaaatatg
    17701 aaaaatttat tgggtgatta aaatgaaatc ttatggaata tgcccagcat cttgtggaga
    17761 atttgtacaa ggtataattg atgatgaaga atatttatgt tcatatgcaa tagatatgta
    17821 ttctaaagtg tatattgaag aaaaattagt agatataaat ttagggagat ataagtcaag
    17881 gttagcaatt gaaaaggtat ttgaaaaatt taatctacct aaaaaatata ctaaaaatat
    17941 ttctttaaat ataaatagta aaatacctgt tggaaaagga atggcaagtt caactgctga
    18001 tataggagct actataaagg caacattatc attgatagat aaagatttaa gtagcgagga
    18061 gatttctaaa ttagcagcag aaatagaacc aacagattct atatttatag ataaaaatag
    18121 tatatttaat cctctaaatg gaactgttat aaaataccta ggaaatctga ccaatgcgaa
    18181 agttgtaata cttgaaccaa acaaagtact agatactatg aaaattagat tgaggcaaga
    18241 ttataataaa ttaaaagtag agaataaaga agtaataaaa aaatcatttg ctcttttaga
    18301 agaaggattg aaaaaaaata atttatctct agttggagag gcttgtactt tgagtagctt
    18361 agcaaatgag aatatagaaa aaaaagagta tttaaatgaa ataataaaaa tatcaaaaaa
    18421 atatggagca tatggagtaa atattgctca cagtggaact gtagtaggta ttttaattga
    18481 taagtttatg aatgataaaa agatgataga tgccttatgt gaatctaaca taaattctgt
    18541 ctataataaa atttatacac aaaacataat taatggagga attaaggggg aaatagaatg
    18601 gaatacataa agaatcctat gaaaatagaa gaaaaaagtt ttgaattgat acaagaaata
    18661 atagacgaaa ttagaccaga ttataaattt aagaataata tcgaagaaaa aataataaaa
    18721 agagctatac atactactgc tgattttgat tacttagata tattaaaaat atcagaagaa
    18781 gcagttgata gtataataga tgcattaaaa aataatgcaa gtatttatac tgatacaaat
    18841 atggctctta gtggtataaa taaaagaaag ttagaatcat taggatgtaa gtataaatgt
    18901 ttagtggcag atgatgaaac tgtaaaatta gctaaagaaa aaggaattac tcgttctatg
    18961 gcagcagtag aaattgcagc taaagaagaa ggaagaaaaa tattcgtttt aggtaatgct
    19021 ccaacagctt tatataaagt tatggagatg aaatcagaag gtagattaga tttagatgca
    19081 gttataggag ttcctgtagg ttttgtagga gcacaagaat caaaagatga agttgaaaaa
    19141 acagatatac catatataat ttctaaaggt agaaaaggtg gcagtaattt agcagcagca
    19201 atagtaaatg ctatattata tactatgtag gtacctatat ggaagaatat gtatatatag
    19261 atggaaaaaa atatagaaga ggatatacta caggttcatg tgcaacaggt gcatctaagg
    19321 ctgctgtata tatgttgatt acaaaaaata gaatcaatac tataaatata gatactccta
    19381 aggggatacc attactacta aaagttgata atataaatat ttctgataca tttgtggaat
    19441 gttccataaa aaaagatggt ggagatgata tagatgcaac tcataccatg gatatttatg
    19501 caagagctga aattgtagct aaaaatgata agaacaaagg gtatcttact ctaaaggata
    19561 tagataattt aagcaccaat agtgagtgta aaagtgagct ctataagttt attagggttt
    19621 atggcggaac tggtattggt gtggttacaa aaaaaggtct tagcgtggat gttggtaaac
    19681 ctgctataaa tccgactcca cttaaaatga taaatcatga aataagaaag cttataggag
    19741 ataattttga atctatactt ggaaatgata aagttttaaa aataacaatc tttgctccac
    19801 aaggagaaac tgtagcaaaa aaaacattta atccaagatt aggtatagtt ggaggaatat
    19861 ctataatagg tacaactggg attgttgaac ctatgagtga tgagggttgg aagaagtctc
    19921 tatctataga acttcagatg aaaaaagaac aaggcttaga taaaataata ttggttccag
    19981 gaaatcatgg agaacagttt ataagagaaa aattaaactt agatattaag tatgtagtta
    20041 gggtaagtaa ttttataggc tatatgatta aagaagctca aagaatagga tataagaaaa
    20101 tcttaatggc aggtcatata ggtaaattta taaaagtatc tgctggtatt tttaatactc
    20161 atagtaaggt agcagatgct agaagtgaga tattagtggc aaacttagct ttaatgggag
    20221 ctagatacga atttttaaat aaaataaatc aatgcgtaac tactgaagaa gctgtagagc
    20281 taataaataa tagtgaatat agagaagtat ataatatatt aagtaataaa tgtagggaga
    20341 gagtaaagca atacttgaat gaagattcag atgatattga tgtggaagta atcatatttt
    20401 caatggataa gtctttatta ggtaaatcgg ataatacaga tgatttagtg gaggtcttta
    20461 tatgataaac ataataggtc taggacctgg taacttagac tatataacaa aaaaaggtga
    20521 aaatttaatt tccacttctg atgtattaat tggtggtaaa agaaatttag agtctataaa
    20581 aaattttgaa ggagaaaaaa tagttctgga ttctaattta agagaaatta tagagtacat
    20641 aaataacaat aaagaaaagc aaatatcaat aatagcatct ggggacccac taatatatgg
    20701 gataggtaga tatctatcta aaaatattga taataagatg ttaaacatgg tttctgggat
    20761 tagttcatta cagtacatat tttcaaaaat atatgtagat atgaatgatg tatatattac
    20821 aagtagtcat ggtaaagtac cagattttga ttacatatta tcacataaaa aagtatgtat
    20881 ggttacagat tcaaagatag gaccaaaaca aataagtaga gaaataatag atagaaatct
    20941 aaataaaatt attgttgtag gagaaaatct gtcatatgac aatgaaaaaa taacaatagc
    21001 aaaaccagaa gagataataa gaatggacaa ttttgatatg aatgttgtgg tgattttaga
    21061 tgaggaatag tgaatttata acaggtaaag ttccaattac taaggaagag gttagagcaa
    21121 tatcaataag taagttaaat ttagtaaatg ctaaaaacct catagacata ggggctggta
    21181 caggaagtgt aagtgttgaa gctgcatata attatcctaa tttaaaggtt atatctatag
    21241 agaaaaatga tgatgctata gagcttatag agaaaaataa aaaaaaattc aacttgaaaa
    21301 atatagaagt gataaaagga tatgctccaa ttaatctagg gttaaatgtt aaagttgagt
    21361 ctatttttct tgggggaaca ggaaataatc tggaagaaat aatagaatgg tctaaaaaaa
    21421 atctcgttac aggaggaaga cttgtagcta attttataat aatagataca tttaatcaaa
    21481 ctttaaaact gcttaaaaag catggattta aagaaataga tgtatgtgtt ttgaatgttt
    21541 ctaaactaga gaaactagga aaaggtgaat attttaaacc actaaatcct atatatataa
    21601 tatcttgtga aaaaggggaa aatgatgatg aataaagtac attttgtagg agcaggacct
    21661 ggagataaag agctgataac attaaaagga tacaaattat taagtaatgc agatgtagtg
    21721 atatatgcag gttcacttgt taatcctgaa cttttggaat actgtaaaga agattgccaa
    21781 atacataata gtgcacatat ggatttacaa gagataatag atgttatgag agaaggtata
    21841 gaaaataata aatctgttgt aagactgcaa acaggtgatt tttctatata tggttcaatt
    21901 agagaacaag tggaagattt aaataaattg aatatagatt atgactgtac tcctggagta
    21961 agttcttttt taggggcagc atcatcttta ggggttgaat atacagttcc agaaatttct
    22021 caaagtgtaa ttataacaag aatggaaggc agaactccgg ttccagagaa agaatcaata
    22081 caatcatatg ctaagcatca gacatctatg gtaatatttt tatctgttca agaaattgag
    22141 aaagttgtgt ctaaattact ggaaggtgga tatccaaaag atactcctat agcagttata
    22201 tacaaagcaa cttgggcaga tgaaaaaata gttaaaggaa ctttaagtga tatagctgta
    22261 aaagttaaag aaaacaacat aaataagact gctttaataa tggttgggag atttttagga
    22321 gaagagtata ataattcaaa gttatatgat aaggatttca aacatgaata tagagggtaa
    22381 tattgctata atctgtatta cagaaaatgg aaaaaaatta gctctaaaga taaattctct
    22441 agtaaataac tgtgttatat atcaagtaaa aaataaaaaa agtaatttag ttgttgaaga
    22501 aacatgtgtg catacagttc aaaagaagtt aagtgatttt gtaggagaaa tatttagcaa
    22561 atttgattac atagtattta taatggctac aggtatagtt gtacgaagta tagcaccata
    22621 cataataagt aagtttagtg accctgcaat tttggtaatg gatgaaaaag gcaaaaacgt
    22681 aataagttta ttgagtggtc atattggagg ggcaaacagc ttgacacagt acataagtag
    22741 tcttattgat gctaatccag taataacaac agctactgat ataaatcaaa aagcttcctt
    22801 agatatgata gcaaaaaaac taaatgcaca tattgataat tttagagaaa gtgtaaaaga
    22861 agtaaattct atgctagtga ataataaatg tgttggatta tatgttgatg aaggatatca
    22921 agttgatact aggggattta aacttataga aagtaaggat gagttaagag atagtttttc
    22981 aaacacatat atagaaaaat tagttgtagt aagcaataaa ataaattgta ttgaagatat
    23041 tgagaattta aacctagata ataaatttga acattgctat agtacccaaa aaataataaa
    23101 ggtaattcca agagatatag tgataggaat tggctgtaga agaaacacag atagtgaact
    23161 gatgtataat tcacttgaag agtttcttaa taaacaaaat atagatatta gagcaataaa
    23221 cacagttgga agtatagaac tgaaaaaaga cgaaaaagca ataattgaat tatcaaaaat
    23281 attgaaggtg ccatttaaaa cattttcagt agatgaaata tctaaggttg ataatctttt
    23341 tgataaatct gattttgtta aaaaaaatgt gggtgtgtat tcagtagcag aacctgtagc
    23401 atatcttttg agcaaaggta atttgttagt tgaaaaacat aaatataaag gaataacttt
    23461 ttcagtgggg agaataaaaa tatgatatac gttgtaggaa ttggaccagg aagtaaagat
    23521 acaatgactt tagaagcaat aaaagcaata gaagattctg aggctattgt tggatataag
    23581 acttatataa aactaataga agaatttata caaggtaaag aaataatcca aaatggtatg
    23641 aggcaagaag ttgatagatg taaacaagca gtggaggaag caaaaaaagg aaaaaaagta
    23701 gcagttgtaa gcagtggtga tgctggaata tatgggatgg caggtcttat tttagaatta
    23761 atatctaaag aagatgaaga tataaaagta aaggtaattc caggcgttac tgcaagtatt
    23821 ggtgcagcag ctatacttgg agctcctatt atgcatgatt tctgtcatat aagtttgagt
    23881 gatttaatga ctccgtggga agtaattgaa aaaagattga gattggctgc tgaagccgat
    23941 tttgtaattt gtttgtataa tccaagaagt aagggaagaa gtgaacatct aagcaaagca
    24001 ttcaaaataa tgggtgagtt taaaggtgga gatacacctg ttggagtggt aaaagatgta
    24061 ggtagagaaa aagaagaaaa gtttgtatgt acttttgaaa atatggactt tgaaaaagtc
    24121 gatatgacta caatggttat aataggaaat aagtctacct atattaatgg tgaattgatg
    24181 ataactccaa gaggttatac agtatgattt taattttagg aggaacttca gatagtttat
    24241 ctatatgtga taagataaat gaactgaaaa atcaaccata tatcctttca gttactacag
    24301 attatggaag ggaacttgca caacaacatg cagagaatgt aattttaggt aagcttggaa
    24361 aagaagatat gctaaaattt ataaaagata ataatatagt aaaaataata gatgccactc
    24421 atccttatgc agttgaagta tccaaaactg ctattacttg tgcaaaacta ttgaatatag
    24481 attatataag gtttgagaga aaatctttaa ttgaagaaat agattatgat aataaattta
    24541 tagtagatac aattgaagaa gcctgtgaaa tagcaaataa aatagggaca aatatattca
    24601 taggaacagg aagtaaaaat ttgaacagat ttatagagga aataagtgat aaaaatttag
    24661 ttgttagagt tcttccaacg agtgaagtca tattaagttg tgaaaaatta ggtttaagtg
    24721 cagataatat agtagctatg aaaggtccat ttagccagta tataaatgaa gaaacatata
    24781 aacattacaa tatagattta gtcataacaa aagaaagtgg aatagctggt gggttcttag
    24841 agaaggtaaa tgcatgtaaa actttcaata taccagtaat aattataaaa agagaatcga
    24901 ttaactacga aaatgtattt aatgacataa ataaaattaa agacatactt gtgtaatttt
    24961 taggaggaga atatatgaag aaagccttat tagtagtgag ttttggtaca agctatcatg
    25021 aaacgagaga aaagacaata gatatatgtg aagataaaat aaaaaattcg ttaaaaagcc
    25081 atgatttttt tagagcttat acatcaaata tgataataaa taaaataaaa aaaagggatg
    25141 gaatagaaat agataatcca atacaggctt tagataaaat atatgaacaa ggatatgatg
    25201 aagtaataat ccaaacactt catattatat gtggcgaaga atttaacaaa ttaaaagaac
    25261 aagtagatgg atattcttct aaattcaaaa agattgtatt aggaagacct ttgcttacac
    25321 atattgaaga ttatcaggaa gctgtagagg caattaagca tcaaattcct catatggaaa
    25381 gtaatgaagc agtagtattt atgggacatg gaacacttca tgaatcacat tcagcgtatc
    25441 cagcattgga atacatgctt agggataatg gaataaatgc ttatgttggt acagttgaag
    25501 ggtatccaga aatagaacat gtaataagaa gattaaaaga aggaaatata aaaactgtaa
    25561 atttgatgcc atttatgtta gttgcaggag accatgctat aaatgatatg gcaggagatg
    25621 aagaagattc ttggaaaaca atattagaag aaaatggatt taacgtaaga gttcatttaa
    25681 aaggactagg agaaaattct tatatacaag ataaatttgt tcgtcatgct gtaaaatgtg
    25741 cagagaatgc gaagttctat ggtataggag cagggccagg agatggttct ttacttacga
    25801 taaaagcggt taatacttta aagaaactgg atattctata cactccagaa tcaaaaaagg
    25861 gtggagatag tctagcttta tcaatagtta gtgaatattt acctgaatca gtggaaataa
    25921 agtctaggca tttcccaatg agttttgatg ggaatgaaaa aagtttagct tgggacaatg
    25981 tggcacaaga aattatatca gatgttaatg atggtaaaaa tgtaggattt gttacattag
    26041 gagaccctat gatttatagt acatatgtat atataatgaa aagaatatta ggagaggtag
    26101 aagtcgaaac tataccagga atttcatcat tttccaatat agcatctaat cagaattttc
    26161 cattagtcat ggataaagaa gctttagtgg taattccttg tactatggaa gatgataaga
    26221 tagagtatgc tttgcaaaat tataattcaa tagtattgat gaaagtatat aaaaatttta
    26281 aagaaataat aaaaaaatta gaaaagtatg atttaataga acatgctatt ttagtaagta
    26341 attcttccca agaaagcgaa aatgtgttta cagatttaaa agaagcgcac cttgaagata
    26401 agatatctta tttttctact atactagtaa ataaagataa taaaataaaa taggtgaaag
    26461 caatatttaa aggagactta atatgaatat agtggttgga actaggggta gtaatctagc
    26521 gcttatacaa acagaatggg taataaatga attaaaaaag aagtatccag aaattagttt
    26581 tgagattaaa atcataaaga caaaaggaga tttaattcaa aatgtttcac tggataaaat
    26641 aggtgataaa ggattgtttg tcaaagaaat agagcaacaa ttattagatg gaaaaataga
    26701 catagcagta catagtatga aagatatgcc ttcttattta gcagatggac taaaatttgc
    26761 tcatacgcct aagagagaag acccaagaga tgttctaata cttagagaag gatataaaaa
    26821 tttagatgat ttgcctcatg gagcagtaat aggaacagga agtaaaagaa ggaagtttca
    26881 attattgaaa caaagacctg atttaaatat agtacaagtt agaggtaatg tggaaactag
    26941 gatacgaaaa ataaaagatg aaaacatgca tggtattgtg ttggcagcat caggaataat
    27001 tagagctaat ttacaagata aaataagttc ttatttacct gttgatgttg ttattccagc
    27061 acctgcccaa ggagctttag ctatagaaat tagaagcaat gatagtacga tagagggaat
    27121 agtaaattca ctgaaggatg agaatacaga aatccaaata ttggctgaaa gaggtttttt
    27181 agatggtgta aatggaagtt gtcatattcc aatggcagct tattgtgaaa taatccaaga
    27241 taaaatacat cttactggct tatatggaga ttcagaagga aaaaaagtag taatcaagtc
    27301 tatagatgga gatatttctt ctcctagaga gttaggttta aagttagcta aacttgtatt
    27361 gaaggagtat gagaattatg aagggtaagg tttacttagt aggggcagga ccaggtgatt
    27421 ataaactcat gactttaaaa ggtttagagt gtataagaaa atcggatgta atagtatatg
    27481 atagattagc aaatagtagt tatttaagag aagctaagcc tgattgtgaa cttatatatg
    27541 taggcaaggc atcaagtaat catattttgc cacaagaaga tataaataga attatagcag
    27601 aaaaagctaa agaaggaaaa attgtaactc gtcttaaagg aggagaccct tatgtatttg
    27661 gcagaggggg agaagaagca gagactttag tagatgaagg tatagaattt gaagtagttc
    27721 caggtataac ttctgctata ggaggtttat gttatgcggg tattcctata acacatagag
    27781 accatgcttc gtcatttcat gtaataacag gtcatttgaa gggtgatgat agtggagaat
    27841 taaactggaa tgctcttgca aataacaaag gtactcttgt gtttttgatg ggaatttcaa
    27901 acctagagaa aataagtgaa aacttaatga aagaaggaaa agataaggat acacctgttg
    27961 ctcttataag ttgggcaact agatataatc aaagagttgt tacatctaca cttgaaaatg
    28021 tttatgaaac tgctattaaa gaagaagtaa aaccgccaac tcttatagta gttgggagtg
    28081 ttgtaggact tagagaaaag ttgaacttct ttgaaagtaa gcctttattt ggaaaaagta
    28141 ttgctgtaac taggtctaga aatcaaaata gtgtattggt agaaaaaata atggatttag
    28201 gtggaaatcc tatagaaata cctactatta aggtagaaaa aatacaaaat aatattaact
    28261 tagaaaatga aataaaaaat ataaataagt ataattatct aatactaact agtaaaaatg
    28321 ccgtagaaat cttctttgaa aagatatatg aaatgaattt tgatttaagg atattatcta
    28381 atttaaaggt atgtgctata ggttctgcta catcaaatga gttgaaaaaa agaggaatta
    28441 tagcggatat agtaccaaaa aaatttgtag cagagtcact gtatgaagag ttagcgccta
    28501 ttttaaatga tgacgataat atacttatac ctagagcaga aaatgctaga gattatctgg
    28561 ttgacaaact caaggctatt tgtgaagtaa cagaagtaca tatatataga acagtaatag
    28621 atgaggagaa aaaaggtgag atattagata ttttaaattc taatgacata gattatataa
    28681 catttactag ttcctcaaca gtaaataatc ttgttgaaat tataggaaaa gaaaatatta
    28741 aaatattaga aaaaataaaa acactatcta taggtcctat aactacagag acaataaata
    28801 gtttgggaat aaaattgtac aaagaatctg ctacatctac tatagattcg ttagtagatt
    28861 taattataaa aaattaattt tggaggcttg aatatgttaa gaagaccaag aagactaaga
    28921 gcaagtaaag caatcagaaa tttggtaaga gaaactaagg tgaatgtaga agacttaata
    28981 tatccactgt ttgtagtaga gggggaaaat ataaaagaag aaattaaatc tttaccagat
    29041 gtctatcatt tatcaataga tatgctagaa gacgaaataa aagaaataag tgagttggga
    29101 attgaacata taatgttatt tggtgttcca gatgaaaaag atgagtgtgc ttcacaagct
    29161 tttaatgata atggaatagt tcaaaaggca attagaaagg taaaagaaat aaattcagat
    29221 ataaatgtaa taacagatgt ctgtatgtgc caatatacaa gtcatggaca ttgtgggatt
    29281 ttaactgata ctggttatgt tgataatgat aaatcattgg agtatttggc taaaatatct
    29341 gtaagccatg ctaaagcagg agctgatatg gttgctccat ctgatatgat ggatggaaga
    29401 atacaagcaa taagagaagc tttagatgaa aatggattag aaaatgttgg aatcatggct
    29461 tatagtgtta agtatgcttc aaattattat ggacctttta gagaggcagc tcattcagca
    29521 ccatcttttg gagatagaaa aacatatcaa atggacccag caaatagcaa tgaagctata
    29581 atagagactg aacttgatat attggaaggt gcagatatac ttatggttaa acctgcatta
    29641 tcatatttag atattataag aagagttaaa gataattttg acttacctct agcagcttat
    29701 aatgtaagtg gagaatactc tatgcttaag tttgctgtga aggaaggttt attaaatgaa
    29761 gatgtaattc ttgaatcaat tacatctata aaaagagcag gagcagatat aataataact
    29821 tattttgcta aagacattgc taaactgttg aaaaaataaa ttctagcagt ataaagaata
    29881 tcaaaaagta taggaattgt aattaactat tatacagtag ttttgtttag acatttattg
    29941 aagaataata gaaactaatt tgcaatttct atactttatt tatgtaacta aataaaattt
    30001 ggaggtagaa gatgctatat cctattaatt taaaattaga tgagttagat gtagttataa
    30061 ttggtggtgg agaagttgca tataggaaat gtaaaaattt tttagagttt aacaaaaatg
    30121 ttactatagt ttctaaacaa attttaaata aattttatga tttaaaagga aatataaaaa
    30181 taataaaaga tgattataaa gaaatatata taaaagatgc ttctgtaata attgctgcca
    30241 caaataatag agagttaaat atggaaatag gattatattg taaagcaaaa aataagctag
    30301 tgaatgtagt tgataatata gaaatatcga actttactgt tccatcatat ataaaaagag
    30361 gagatttact tataagtgtg tctactgggg gcaagagtcc ttcattatca tcaaagataa
    30421 aaaaagaaat tgaagagaga tatactgaag attatgagga gtatttaaat gtattaggag
    30481 atataagaaa aaaggttata aaaaaatacg aagataaaag taaaagaaaa gatgtgctta
    30541 atatgttggt aacacttgac ctagaagaac ttaagaagtt tgatatataa aaaataaaat
    30601 aaaaaatgtt aaaaaggtgt tgacggatta tttttaagat gatatagtat tacttgtcct
    30661 taagaaaagg gcaaacataa
//
LOCUS       DAYAPA010000038        30233 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.038, whole genome shotgun sequence.
ACCESSION   DAYAPA010000038 DAYAPA010000000
VERSION     DAYAPA010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 30233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30233
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.038"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            <1..1143
                     /locus_tag="ACGI59_001599"
     CDS             <1..1143
                     /locus_tag="ACGI59_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HFG9739388.1"
                     /translation="ISNNAVDSKLMNSRIKSIDIIRGLSIALMIVCNNPGTWMRMYPQ
                     LRHAVWHGVTLADFAFPFFVISLGVTIPISINSKLKNNKSTLSIILSIFKRSILLILF
                     GFFLNYLGNPDLDTVRILGVLQRMGLVYFVTSLVYLLLKKLNVGSTATIITFLCISTF
                     IIVGYYILAKPYGFELEGSLAQLVDLHFFKGHLYKPEFEPDGFLTSIVAISSGMLGCT
                     MGCVLLKENIGEYKKFFKILVMSIILLIGAFIFNQYFPFNKRLWSSSFVLLMAGSYGI
                     LLSIFYFICDIKNKSKIFTPIIALGSSPIFTYMCLEILSHVFWNVPKLTNKVDYPTTL
                     VEWTTYELITPWAGTTWDSLIFSLLYVLFWVIVMSIMYKKKIFIKI"
     gene            1396..3420
                     /locus_tag="ACGI59_001600"
     CDS             1396..3420
                     /locus_tag="ACGI59_001600"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFG9739389.1"
                     /translation="MKKTTKLLATGMLSVAMVAPNVALAAENTTANTESNSDININLQ
                     RKSVVLGSKSNASVKFKEKLNADSITLNFMCYDMPLEATLNYNEKTDSYEGVINYNKD
                     PEYLNVWELQSIKINGKDEQKVLNKEDLESMGLNLKDYDVTQEFIISDANSTKAVNEY
                     MRKTSAPVKKLAGATRFETAVEISKQGWKDGSSKVVIVNGELAADGITATPLASTYDA
                     PILLANKDDIPESTKAELKRLNPSDVIIIGDDGSVSQKAVSQIKSAVNVNVTRIGGVD
                     RHETSLLIAKEIDKYHDVNKIYIANGYAGEYDALNISSKAGEDQQPIILANKDSVPQG
                     TYNWLSSQGLEEAYYIGGSQSLSSKIIDQISKIAKNGTSKNRVSGADRHETNANVIKT
                     FYPDKELSAMLVAKSDIIVDSITAGPLAAKLKAPILITPKTYVSAYHSTNLSEKTAET
                     VYQIGDGMKDSVINSIASSLSKHNAPTEPDNSGSAAGKTVVIDPGHGGSDSGATSGLN
                     GGAQEKKYTLNTALATTEYLRSKGINVVMTRDTDKTMALGERTALSNTIKPDLFTSIH
                     YNASNGSGNGVEIYYKVKDKNGGTTKTAASNILKRILEKFNMKNRGIKTRTLDNGKDY
                     LYVLRNNNYPAILVECAFIDNKSDMDKLNTAEKVKTMGTQIGIGIEDTVK"
     gene            3640..4326
                     /locus_tag="ACGI59_001601"
     CDS             3640..4326
                     /locus_tag="ACGI59_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFG9739390.1"
                     /translation="MSGYTNDECEIPKIKSYPGADKEIASEIDYSIVKGTVLFDLYQR
                     IMDLVLSIIGLVIGLPLIAIFGILIKIEDKGPITYKQERLGKCGRRFYIYKLRSMRTD
                     AEKFGAQWAEKDDPRITKVGKFIRKTRIDEIPQLFNILKGDMGLIGPRPERPNFTVQF
                     NEEIPGFINRLAIKPGLTGWAQVNGGYEITPEEKLKEDIYYIKNRSILLDFKILFKTV
                     KVVLTGDGAR"
     gene            4421..5476
                     /locus_tag="ACGI59_001602"
     CDS             4421..5476
                     /locus_tag="ACGI59_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp7"
                     /protein_id="HFG9739391.1"
                     /translation="MIKKISTILSLVLLISISSTIGVFADANPKRELIEGSIPEISTE
                     LNKRAFKDSKEVILVNEESIVDSISATPLAYAKNAPIVVTKSKNLGRVTRNYLKELGP
                     EKVTIVGGLKAVSKDAERNIEKMGMKVERIRGKDRYDTSLKIAREMYRTVGFDEAFLL
                     SSTTGLENAISVYSYAAKSGMPIIWAKDEGFEEQIDFLKGKNLKKIYALGDSKEFIAE
                     IDSNLKNIEGIKQINKSSTNVDLIKKFYDEKDIKKIYTARLDFGSRSDVNEYISLGVV
                     SAKENMPILICSDNLSRAQDKFLKDSNINDVVEVGYTVGDYSLFKSIFNLTFLSCIVL
                     ILLLLLITFRALRYESK"
     gene            5591..7147
                     /gene="murJ"
                     /locus_tag="ACGI59_001603"
     CDS             5591..7147
                     /gene="murJ"
                     /locus_tag="ACGI59_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HFG9739392.1"
                     /translation="MSKTAKAALWIMAATMFSKVLGFLRELVLANFYGTGMYADVFVL
                     TLNIPGLIIAVIGSAVATTYIPMYFETKKRLGDEGALKFTNNVLNICYIMAIVIAIIG
                     LLFTEQFVTVFAAGFRNDPAKFQAAILFTKIMISGVLFLSGSKIFSSFLQVNDSFVIP
                     GLIGIPYNIIIIAAIALSAGKNVWILPAGALLAMASQLLFQLPFAFKKSYKYKPYINL
                     KDESIKELVNLVLPMLVGVAVGQLNIFVDRLLATTLGDGKLSALNYANRLNEFVMALF
                     VTSIITVIYPKLAKMSGKDNKEGFISTIVKSSNCIILVVLPISIGAIILAEPLVRILF
                     QRGKFDALSTDLTSIALRLYSLGLLACGVRDVLYRAFYSLSDTKTPMINGSIALIINI
                     VLNLILIRPLGHAGIAISTSTSNIITVILLFISLKKKNGYFGGDKIIKTGLKSLVASG
                     VMAVATLLIYNNLYAFMGSGTIKEIISVGAGVLGGASVYTVLIVLFKVEEMDLAFEFL
                     RKGKQKLLRR"
     gene            7193..8899
                     /locus_tag="ACGI59_001604"
     CDS             7193..8899
                     /locus_tag="ACGI59_001604"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HFG9739393.1"
                     /translation="MDYKNNYEMWLNSPYFDEQTKNELLSIKDDEKEIQDRFYKNLEF
                     GTGGLRGIIGAGTNRINIYTVRRATLGVLNYIMKTQGEEGKQKGIVIAHDSRYMSREF
                     CIEVAKTLSAYGVKAYIFEELKPTPELSFAVRYLKCAMGIVITASHNPKEYNGYKVYD
                     SDGGQICIDMANDIIAEVNKIDDYSTIKSIDFEEALSKNLITILDNEVDDEFIKAVKK
                     QVLRQNIIDEYGKKLKIIYTPIHGTGNKPVRKVLNECGFENVMVVKEQELPDSNFSTV
                     KYPNPEEKSVFNIAIEMAKNNGTDLIIGTDPDCDRVGIVVKDSSGEYVVLNGNQVGSL
                     LVRYILESLVEENKLPKNNPTIIKTIVTSELGAKIAKAYNVDCLNTLTGFKFIGEKIK
                     AFEESNDRSFIMGYEESYGYLIGTHARDKDGVVSSLMICEMAAYYSSKGMNLYEALID
                     TYNKFGYYKEDLKSVTLKGIDGIKKIKEMMLYFRSVKIDNVADVKVDKILDYKDGVDD
                     LPKSDVLKFLLEDGSWIAIRPSGTEPKIKFYFGANSDNQEDVEFKLNNLISYILNVVD
                     SI"
     gene            8921..9994
                     /locus_tag="ACGI59_001605"
     CDS             8921..9994
                     /locus_tag="ACGI59_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HFG9739394.1"
                     /translation="MKVYNVIMAGGGGTRFWPLSRQEVPKQLINLSGEDALINETINR
                     IDSLAKKDDLFIVTNEKQLEALKDIVKDKCLDSNILPEPCARNTAAAIGFAAFNIMKK
                     YGDGVMCVYPADHYIKDEKEFKSILEKAIYIAENNDKLVTIGITPTFPSTGYGYINFN
                     RENTIEDVAYEVVEFVEKPNYEIAKEYVNSKKYVWNSGMFVWKVSKILEDFKRYLPKV
                     YEKLEDISKYLGTKEEMEKIKEIYPTIQSISIDYGIMERSNDVIVVPGDFGWNDVGSW
                     DSLGAIYPTDDEGNIKRGENITIDTKNSIIYSDDKLISTIGISDLIVVSTNDAVMVCR
                     KDKAQDVKKIVEQLKEEDRQEYM"
     gene            10006..10728
                     /locus_tag="ACGI59_001606"
     CDS             10006..10728
                     /locus_tag="ACGI59_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFG9739395.1"
                     /translation="MIPKKIHYVWFGGPKGNIENICINSWKEKLPEYEIVEWNEKNFD
                     IEKEIKGNKFLEECYKRKLWAFISDYTRIKVLYEQGGVYMDTDMQILKDITPLLENNR
                     LICGYEDDREYINGAIIGVEKGHPFLKDLLEYYEKEVLTSSLFTIPKIMTHLMEKNYK
                     KIDPNNYEEGIRVYDKEYFYPFGFKEDFTPECITENTFGIHWWGKSWAKKRNYFLESK
                     HLTGVNKIWKCCKIFASNTLRS"
     gene            10744..11901
                     /locus_tag="ACGI59_001607"
     CDS             10744..11901
                     /locus_tag="ACGI59_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide polymerase"
                     /protein_id="HFG9739396.1"
                     /translation="MKTLNDDRNIMIRKYLYIFVLFIILLRKYMISFIDPNMDIGMIK
                     SALFYSSIGILMLLFLFDKRKSITEMVLVGVCVLLYLLNREGAILLIVLLAVSAKQID
                     DKFIVKNYLIISACFLMVAILLFNLFPSLIFNQEVHYRYIEKIDMLVTRMDFGLGNPN
                     SVFYHMVTIYAAYIFLRYKDYNKWDRIILFGSAFFVYQTTYSRTGFFTILAGLIFVEI
                     IRWIDIKKIKGLPMLLKTLPIILTLFSVIIGTVFDKSTLLNRLLASRPKFWHVYLAEK
                     GNFLKPFGNSYSPAIKATNPLDSSYVYIISVLGVVACILFMYLMYKGIESFIEKDKKA
                     YLVALFIFLLYSFAENLLLEASFSFAVVLLIKEVILNDKREIDLWKMKSRR"
     gene            11904..12743
                     /locus_tag="ACGI59_001608"
     CDS             11904..12743
                     /locus_tag="ACGI59_001608"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFG9739397.1"
                     /translation="MLISLIMPTLNRYDDIYLLMDSLENQTYKNFELIVVDQNDNSKV
                     KEIVDKYIDKLDIKYIKSSKKGLSYNRNVGIDNAVGQIIGFPDDDCVYENDTLEKVIN
                     FFNKNKDYKIYSCKTMDSNKVDAFKKMYDGTCDITSSNVLDTITSITFFIDFEGKDYT
                     RFDEKLGVGGEFGAGEEIDYVLNLLSLGFKGKYFGNDIIYHPAKKHSKSKEKYQKDYN
                     YGRGFGALCKKEIVYRKNYKFAKVMVSKLVRNIGGLILSSNRDYHSATIKGRINGFRQ
                     YKL"
     gene            12758..13933
                     /locus_tag="ACGI59_001609"
     CDS             12758..13933
                     /locus_tag="ACGI59_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HFG9739398.1"
                     /translation="MDKVKFLINMLLAFFIYPFNKHKFNGRDIWLIGGHSGDIYNDNS
                     KFFYEYMLKEHNDVETYWVVNKDSKVFDKIPGKKLIRGSVENYLYYYNSKAIVFSHAP
                     SADIAPYNFAVPVLNYFHKKTIKVFLNHGTISFKKRKPMNKKFKNIIDNLYKSYNIVT
                     ASSEFERNVMVNDWGMLDDSVYIIGNARYDNLPTNEVAQTRDILYTPTWRDWIKFSSG
                     KFTDTDYFKNIMNFLNDDKLNKILDEKDINVKIYMHHLMHEFIDDIKENITGKRIVFL
                     DKGVTLANEIRKSAANITDYSSVAIDFLYMNRPILFYQFDLDEYMEKVDSYIDLKSEM
                     FGSLAYNNDEAVNKLIDIIENNFEVMDNQKNERNKFFRYNDNKNCKRIYDCVLSKIK"
     gene            13962..14711
                     /locus_tag="ACGI59_001610"
     CDS             13962..14711
                     /locus_tag="ACGI59_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFG9739399.1"
                     /translation="MKKNLVSIITPMYNSEKFIEATIKSVLNQTYQEWEMLIIDDCST
                     DNSPNIVKSYMQQDSRIKCIKTETNKGVSNARNLALSKATGQFIAFLDSDDQWNSSKL
                     EKQVNFMLENDYVISFTSYELMDENDKKLNKVIKVPPNVDYRRLLKGNILGCLTVVID
                     KSKLDFEIRMSGVRHEDYVLWLSILKKGHIAHGINEVLALYRKSSNSLSGNKIKAAMW
                     TWNIYRNIEKIPLYKAIYYFINYGINGIKKS"
     gene            14733..15641
                     /locus_tag="ACGI59_001611"
     CDS             14733..15641
                     /locus_tag="ACGI59_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFG9739400.1"
                     /translation="MYKYKFTVFTPTYNRAHLLENLYNDLKAQTYDFNDFEWLIVDDG
                     SSDSTKELVEKFISENKLNIRYIYKENGGKHTAINLGVKNADGELFFIVDSDDGLIKD
                     SLEIANDEWDSLENKEGFSGVVGLCVYPSGDLIGTEMPEDKKICHFADLYFKYGVKGD
                     KSIVFVTDELIKFPFPERKEVRFLPESVVWNEMSKYYKVICVNKPMIIRDYLDDGLTK
                     NILSKNALRGRALEFLYLINQNTYPLSRYPYMWIKNYINLARYSLLSDSHYFGELRKV
                     SDKLLYLALFPLGYYKYIGQRKLVSK"
     gene            15689..16441
                     /locus_tag="ACGI59_001612"
     CDS             15689..16441
                     /locus_tag="ACGI59_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFG9739401.1"
                     /translation="MNEPLVSIITPVYNSEEFLSETIKSIQNQTYKNWQLLLVDDCSK
                     DNSSEIIKSFRKEDARIKYIKLEKNSGAAVSRNVGIKNAEGRFIAFVDSDDLWDSRKL
                     EIQIEYMLKENVGFSFTSYRYMRQDGSKTNKVARAPKKIDYEGLLRNTIIGCSTVVID
                     KEIVGEFSMPLVRRGQDTATWLQLLKKEKYAYGIQEDLVNYRLVGNSISSNKIKALKR
                     TWNTYRNVENLSLPKSLYVFCFYVFNAIKKRV"
     gene            16395..17762
                     /locus_tag="ACGI59_001613"
     CDS             16395..17762
                     /locus_tag="ACGI59_001613"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HFG9739402.1"
                     /translation="MYFVSMFLMPLKKEYKFYILGGKKMKIAIAGTGYVGLVTGVCLA
                     EVGNENVVCVDVDEQKVKSMKLGIAPIYEEGLEELMVKNYNRGRIDYTTDYKKAYEDV
                     DVILIAVGTPERSDGSANLDYIKAVAKQIAETVTKDTLVVIKSTVPIGTNEEIEDYIN
                     RNLKNDVKVELASNPEFLSQGTAVKDTLHASRIVIGVESEWAREILEEIYKPFNQPII
                     VTNRNSAEMIKYASNNFLALKISFMNDIANLCEIAGANIEDVALGMSYDDRIGSKFLK
                     AGIGYGGSCFPKDTKALHWLSEHNGYELKTVKAAIEVNESQKLKLVRKASKLVESFQD
                     MKVAVLGLTFKPDTDDIREAPSIPNIKYLLERGARVTVYDPIGVNNTKKIFGDSILYA
                     DSVDDAIEDAEICFIMTEWKDVLNYDIHKYKEKMKNPLVLDGRNCYKIKDMEELGIEY
                     YSIGR"
     gene            17768..18859
                     /locus_tag="ACGI59_001614"
     CDS             17768..18859
                     /locus_tag="ACGI59_001614"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFG9739403.1"
                     /translation="MKTVCYLADGSNPHTLKWCSFFKNKGYDIHVISLNGGHMEGIKI
                     YNFGSNVEELKNENISKKTGYLGSIKQIKKLVNEIKPDILHAQYASSYGFIGSLLGYH
                     PYIISVWGTDIYDFPNNGFIQKQIIKHNFRKADYIFSNSRDMAKEANKYTAKHVDVTF
                     FGVDMDRFKPMEVEKEDAFVIGIIKSLEKKYGIEYLIQAFKMLKDEYKDKKIILKIGG
                     SGSQMDNLINLAKELGIENDVQFLGRISPENVSKTFNSFDVTVFPSLREGFGVAAIES
                     EACEVPVIVTNVGGHPESVWENETGLIVEPKQPEEIKNAIIKLMENDELRLNMGKKGR
                     QFVRENYEVNLNFNDIEKIYDSIFDKYKK"
     gene            18943..20856
                     /locus_tag="ACGI59_001615"
     CDS             18943..20856
                     /locus_tag="ACGI59_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HFG9739404.1"
                     /translation="MRESIDLEKESFFNRLFKKYKFRHLTLLILDICSVLVAFRMSLS
                     LTGNLNAFTTNDIIISACIYIVLHIVSFRLFKCYNTLWRYAGEEEIISIFVATLAYLI
                     PIYIINKLLGFDYPIMFYVLNTIFIIMFTSGARIAYRAIRIVMNKTYSRGKVSNILII
                     GAGDAGEMVIQELKRNPELKKVAVAIIDDDKNKIGRIIHNVKIVGTTSDIKDVVEKYN
                     VDEIIFSIANIEKRRKKEIIDICKNTNCKIKTIPGIYEIIDGKVDIKQIREVEIEDLL
                     GREPIKTNLREISNYIEGKVILITGGGGSIGSELCRQIAGFNPKELIIVDNYENNAYS
                     IQQELLRKYKNKLDLKTVIASIREEKRMDEIFNKYKPEVVFHAAAHKHVPLMESSPGE
                     AIKNNIFGTLNIAGLSSKYRAKKFVLISTDKAVNPTNIMGATKRAAEMIIQTMNAESQ
                     TEFVAVRFGNVLGSNGSVIPLFKKQIEDGGPVTVTHPDIIRYFMTIPEAVGLVIQAGA
                     MAKGGEIFVLDMGEPVRILDLAKNLIKFSGFEPDVDIKIEFSGLRPGEKLYEELLMSE
                     EGLLDTEHKKIFIGRPIDVDREKITKYLKLLREITNNEEVEKIDGIMRELVPTYIKPE
                     DANIKEIATTREK"
     gene            21055..21762
                     /locus_tag="ACGI59_001616"
     CDS             21055..21762
                     /locus_tag="ACGI59_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFG9739405.1"
                     /translation="MMNIKNKKHILKKFIAMVLIAGVVTVEAGAITASAAEPTNSPMS
                     ATVDQCDFLNVRSGASANDAVVGKINTGDKVEVLELHSNGWIKIKSVDNVTGWVNGDY
                     LTIQGGNVDAKVQNVLNLAFKQQGKPYKWGATGPNSFDCSGFTSYVYKNGAGVNLPRV
                     SRSQATVGKKVSRAELKPGDLVFFGSGGSINHVGLYVGDSKFIHSPQTGDVVKVTSMA
                     PGTNYARRLITATRVLQ"
     gene            22064..24175
                     /locus_tag="ACGI59_001617"
     CDS             22064..24175
                     /locus_tag="ACGI59_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding glycosyl-hydrolase Cwp19"
                     /protein_id="HFG9739406.1"
                     /translation="MKKISILVLSLIMTLTMCSVSSFADSSNDKEMRAAWISTVYNLD
                     WPKTKNNEAKQKKEYTDLLDKLKSVGINTAVVQVRPKSDALYKSNINPWSEYLTGTQG
                     KDPGYDPLPFLIEEAHKRGMEFHAWFNPYRITMADESIDKLPANHPAKKNPSWVVKHG
                     NKYYYDPGLPEVRKYIVDSIAEVVQNYDIDGVHFDDYFYPGVSFNDTATYQKYGKGQN
                     KDDWRRENVNTLLRDVKASIKSIKPNVVFGVSPAGIWRNKSSDPTGSDTSGNESYVGT
                     YADTRAWIKQGLIDYVVPQLYWPIGLKAADYSKLVAWWANEVKGTNVDLYIGQGIYKQ
                     GQSSYGGQNIAKEIVQQVTLNRKYSEIKGSMYFSAKDIANSTSIQKDLKSLYSSSEEP
                     VTPPSNVKVEKLRGDERYDTAVAISKKGWATNSDTVVLVNGYSIVDGITSTPLATSND
                     APILLVNKDNIPTSTKNELKRLNPSKVILIGGNNSIGDKVESEIKDTLSNVSINRVGG
                     SDRYSTSLMIAKELVKTNPVEKLYITSGTGEADSLSIASKAGEEKQPIVLVSKDNVSD
                     EVYNWISDLKVKDAYFIGGNLSISDSVINKLDKVITNDVSKNRIAGENREETNGKVIQ
                     KFYPNAEYSSMFVSKSNQLVDALTSGPLAAKLKSPVVMLGNSVTSAQKTALEPKKTTL
                     VYEAGDGINQNTLNTFLNLVK"
     gene            complement(24373..25338)
                     /locus_tag="ACGI59_001618"
     CDS             complement(24373..25338)
                     /locus_tag="ACGI59_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFG9739407.1"
                     /translation="MSKLKKFVILLAFLVVIFPISVYGYFYYKLSAIHDSSISSDLLD
                     NNDHKNEDGIINILLMGTDARPNEDSSRSDAMMILTIDNKHNDIKLTSLARDSYVDIP
                     GHGKQKLTHAYAYGQADLLIQTIEENFNIDIQNYACVNFESFMYIIDAIGGVEVTVEK
                     GEIRELNKFIPETYKWNKSDDKGSIQYIRNSGKQTLNGYQALSFARIRHNDTAFARDG
                     RQRQIIQAIIKKTETLPVTKYPGLLDAVLPYVKTNMKPNAILSLGAQVLKMGDLNIKQ
                     FEFPIDDEIHSTGGIYGKAGWVLRFDPDTLDILHDFIFNDIEFKQ"
     gene            complement(25623..26579)
                     /locus_tag="ACGI59_001619"
     CDS             complement(25623..26579)
                     /locus_tag="ACGI59_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFG9739408.1"
                     /translation="MSGLKKFVIFLALLVIAFPVSIYGYVFTKLKTIQDDSINTEALS
                     SNDYKNEENITNILLLGTDARPGEEVSRSDAMMILTIDNKNKSLKLTSLNRDTYVNIP
                     GRGEQKLTHAYVYGQANLLIETIEENFQLDIQNYAVVDFFSFMDIVDALGGVTVTVDK
                     SEIRELNKFIPETYKWNKNDNKGSLKYIRNSGEQKLNGYQTLAFSRIRKNDSTDERDR
                     RQRSVIQSLINGVKDLPVTKYPNLVNTILPYVKTNMNPNEIISLGKELLSIGNLNLKS
                     MEFPLSTENGRKIGNAGYVIPFEEYELDAMHDFIFKDIMVED"
     gene            26947..27735
                     /locus_tag="ACGI59_001620"
     CDS             26947..27735
                     /locus_tag="ACGI59_001620"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFG9739409.1"
                     /translation="MIKLIATDLDGTLLDEKSEINPEFYKVFKKLRERGIMFSAASGR
                     QYQNLIKKFEDIKDDMMFISENGTLVVYKGKEILSNPLNKELVNEIIETTRSIKGKKI
                     VMSGKKYAYIESKDEAFIQEVSTYYAKFKIVEDLTKVEGDILKIAVFDFKGAEHNNNI
                     YFEKFSDRAQVCISGVEWLDLTAKGANKGSAIKKVQKMLDIKYEETMVFGDQLNDVEM
                     MKSAYHSYAMENANEHLKQIARFRAKRNTENGVVDKIKEVIKIG"
     gene            complement(27858..28328)
                     /locus_tag="ACGI59_001621"
     CDS             complement(27858..28328)
                     /locus_tag="ACGI59_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4358 domain-containing protein"
                     /protein_id="HFG9739410.1"
                     /translation="MKKILILFFSIILSLGMVSCSSSGDYKNVSTSKIKKALKSSDLL
                     IKESKIFDAKDFNYFNDVEEDIIQGFVINSSEKLSLEDIIVIKTDEVDKIYKTLENYK
                     KDMIQAPFGEGHGEKINSEIANNTIIEKAGRYVYLISAENATQIENKILNIIKK"
     gene            28467..29111
                     /gene="uppS"
                     /locus_tag="ACGI59_001622"
     CDS             28467..29111
                     /gene="uppS"
                     /locus_tag="ACGI59_001622"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HFG9739411.1"
                     /translation="MRIPKHVGIIPDGNRRWASQNGMTKDKGYESGIDPGLESYRLCR
                     DLGCEEVTFYGFTTDNTRRPSVQTKAFTKACIDAANILIDEGAALLVVGNTKSPMFPE
                     ELLPYTNRIKAKEGQIKANLLVNYGWYWDLNNISGVENVNKNNIQDYIQSFDVSRLDL
                     IIRWGGRRRLSGFLPVQSIYADFYVIDNYWPDFKSDDIKDAFSWYDTQDVTLGG"
     gene            29235..30059
                     /locus_tag="ACGI59_001623"
     CDS             29235..30059
                     /locus_tag="ACGI59_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFG9739412.1"
                     /translation="MSKGNNNNNSRNKSKKTSHLNRKKRKLNKKKLAVLICFTVLFLF
                     IAFKATQGVVALVKSMDKSNKTNQQQNVNSEQFDLGNEEENKKKKYTVFIDPGHGGND
                     KGTESKTSNRYEKDLNLQIAKKLANKLSKQKDIQVVVSRTDDTYISLKDRAILANNSS
                     ADVLVSIHLNAEKNGNTATGIETWYRNKATDGSKELAQTVQSTIVSYVKVRDRGIVEN
                     NFEVLRESNMPAILIECGFLTTPSEEQKIINEKYQDQLAEGIVQGVLSYLDSKGNK"
BASE COUNT        11984 a         3298 c         5369 g         9582 t
ORIGIN      
        1 atttcaaata atgctgtaga ttctaaactt atgaatagta ggattaaatc aatagatata
       61 ataagagggc tatcaatagc cctcatgata gtctgcaata atcctgggac ttggatgaga
      121 atgtatcctc aattaagaca tgctgtatgg catggtgtaa cacttgcaga ttttgcattt
      181 cctttttttg ttatatcttt aggtgttaca attccaatat caataaatag taaactaaaa
      241 aataataaat ccacactgag tataatactt agtatattca agagaagtat cttattgata
      301 ttatttggat ttttcttaaa ttacctagga aatcctgatt tagatactgt aagaatactt
      361 ggagtacttc aacgtatggg attagtctat tttgtgacta gtttagtata tttactacta
      421 aaaaaattaa atgttggaag tactgctaca ataattactt ttctatgtat atctacattt
      481 ataatagttg ggtactacat attagcaaaa ccttatggat ttgagctaga aggttctctt
      541 gcacagttag tggatttaca tttcttcaag ggacatttgt acaagccgga atttgaacca
      601 gatggttttt taactagtat agtagcaatt tcttcaggta tgttgggatg cactatggga
      661 tgtgtacttt taaaagaaaa cataggagaa tataaaaaat tctttaaaat actggttatg
      721 agcataatcc tgctaatagg tgcttttatc tttaatcagt attttccatt taataaaaga
      781 ttatggtcta gctcatttgt acttttaatg gctggcagtt atggtatatt actgtcaata
      841 ttttatttta tatgcgacat caaaaataaa agtaaaatat ttaccccaat aatagcactt
      901 ggaagtagcc ctatttttac atatatgtgc cttgaaatat tgagtcatgt tttttggaat
      961 gttccaaaac ttacgaacaa agtcgattat ccaactacct tggtagagtg gactacatat
     1021 gaacttataa caccttgggc aggtacaact tgggattctc ttatattttc tttattgtat
     1081 gtcctattct gggttatagt aatgtctata atgtacaaga aaaaaatatt tattaaaatt
     1141 tagtgcaata tttttagatg ataaagtctt tgatttgttc aaatatacgg aaactatcaa
     1201 atataatcaa aatttctcct cgataaaatt taaaaaccat gccataaaag attacaaaaa
     1261 gtttacaatt tgtaattttt gtgctataat gaaaaaagat ttacataata ttgaatttat
     1321 gtaatataaa acaagcttaa gatgtattaa aaaagattta aagatttaaa gaaccaaaaa
     1381 attagggggg atatgatgaa aaaaacaaca aaattattgg caacaggaat gttgtctgta
     1441 gcaatggtgg caccaaatgt agctttagcg gctgaaaata ctactgcaaa tacagaatct
     1501 aattcagata ttaatataaa tttacaaaga aaaagtgtag tgttaggaag taaatctaat
     1561 gcaagtgtaa aatttaaaga aaaattaaat gctgattcaa taacattaaa ttttatgtgt
     1621 tatgatatgc cacttgaagc tactttaaat tacaatgaaa agacagattc atatgaaggt
     1681 gtaataaact ataacaaaga cccagaatac ttaaatgtat gggaacttca aagcataaaa
     1741 ataaatggta aagatgaaca gaaagtttta aacaaagaag acttagagtc tatgggatta
     1801 aatcttaagg attatgatgt tacacaagag tttataataa gtgatgcaaa ttcaacaaaa
     1861 gcagtaaatg aatatatgag aaaaacttct gcaccagtaa aaaaacttgc tggagctact
     1921 agatttgaaa ctgctgttga gataagcaaa caaggttgga aagatgggtc tagtaaggtt
     1981 gtaatagtta atggagaatt agcagcagat ggaattacag ctactccact tgcatctaca
     2041 tatgacgcac caatattact tgcaaataaa gatgatatac cagaaagtac aaaagcagag
     2101 ttaaaacgtc taaatccaag tgatgtaata attataggtg atgatggttc agtttcacaa
     2161 aaggcagtgt ctcaaattaa atctgctgta aatgtaaatg tgactcgtat tggtggagtt
     2221 gatagacatg aaacatcttt acttatagca aaagaaatag ataagtatca tgatgttaat
     2281 aaaatataca tagcaaatgg atatgctggt gaatatgatg ctcttaatat ttcatcaaaa
     2341 gctggtgaag accaacaacc tataatatta gctaataaag attctgtacc acaaggtaca
     2401 tataattggt tatcctcaca aggtttagaa gaagcttatt acataggtgg tagtcaaagt
     2461 ttatcatcaa aaattataga ccaaatatct aaaatagcaa aaaatggtac ttcaaaaaat
     2521 agagtttctg gagcagatag acatgaaaca aatgcaaatg taattaagac tttctatcca
     2581 gataaagagt tgagtgcaat gttagttgca aaatctgaca taatagttga ttcaataaca
     2641 gcaggacctt tagcagctaa attaaaagct ccaatactta taactccaaa aacttatgtt
     2701 tctgcatacc atagtacaaa cttatcagaa aaaacagcag aaacagtata tcaaattggt
     2761 gatggaatga aagatagtgt tataaattct atagcatcta gtttatcaaa acacaatgca
     2821 ccaacagaac cagataattc aggttcagca gcaggaaaaa ctgtagtgat agacccaggt
     2881 catgggggtt ctgattcagg ggcaacaagt ggattaaatg gtggagcaca agaaaagaaa
     2941 tatactctta atacagcatt agcaactact gaatatttac gttcaaaagg tataaatgtt
     3001 gttatgacta gagatacaga taaaacaatg gctttaggag aaagaacagc attatcaaat
     3061 actataaagc cagatttatt tacaagtata cattacaatg cgtctaatgg atctggtaat
     3121 ggtgtggaaa tttattacaa agtaaaagat aaaaatggtg gaacaactaa gactgcagca
     3181 tcaaatatct taaaaagaat actagaaaaa tttaatatga aaaatagagg aatcaagaca
     3241 agaacacttg ataatggaaa agattaccta tatgtgttaa gaaataataa ttatccagca
     3301 atacttgttg aatgtgcatt tattgacaat aagagcgata tggataagtt aaatactgca
     3361 gaaaaagtaa aaacaatggg aactcaaatt ggaataggaa tagaagatac agtaaaataa
     3421 aatagaaaat attaaagcaa aacatatcat ctcctgttat aatgtaaaat ataacaagag
     3481 atgatatatt tttgtaaaga atcaaattgt ttacaatgat gtttcttgta ttgataatct
     3541 gctcattatt gagtataata tagaaataag tcaaaaataa gatagaaata agttgaaatt
     3601 aagaattaaa aaaattttta ttccaatatg gaggtaacta tgtctggata tactaatgat
     3661 gagtgtgaaa tacctaaaat taaaagctat ccaggggcag ataaagaaat tgcaagtgag
     3721 attgactata gcatagtaaa aggaacagtt ttattcgact tgtaccaaag aattatggat
     3781 ttagttttat ctataatagg acttgtgata ggtttacctt taatagcaat atttggaatt
     3841 cttattaaaa tagaggacaa aggaccaata acttataagc aagaaagact aggaaaatgc
     3901 gggagaagat tctatatata taaattgaga tctatgagaa cagacgcaga aaaatttggc
     3961 gcacaatggg ctgaaaaaga tgatcctaga ataactaagg ttggaaagtt tattaggaaa
     4021 actagaattg atgagattcc acagctgttt aatatattaa agggggacat gggcttaata
     4081 ggtcctagac cagagagacc gaactttact gttcaattta atgaggaaat acctggattt
     4141 atcaacaggc tcgctataaa acctgggctt acaggttggg cacaagtaaa tggtggatac
     4201 gaaatcactc ctgaagaaaa acttaaagaa gatatttact atataaaaaa taggagtata
     4261 ttacttgact ttaagatact ctttaaaact gttaaagtag tattgacagg agacggagct
     4321 agataatttt aaaatattag attactaatc aatgaaaaag ctctttaaaa agagcttttt
     4381 tattgtcaaa aaatacaaaa aagaaaagga atgagaactt atgattaaaa aaatttctac
     4441 tatattatcg ttagttttgt taatctctat ttctagtacg ataggagtat ttgcagatgc
     4501 aaatcccaaa agagaattga tagaagggag tataccagag atttcgacag aattaaataa
     4561 aagagcattt aaggattcta aagaagtaat tcttgtaaat gaagagtcaa ttgtagatag
     4621 tataagtgca acacctttag catatgcaaa aaatgctcca atagttgtaa ctaagagtaa
     4681 aaatcttggt agagtaacaa gaaattatct aaaagaacta ggtccagaga aggttacgat
     4741 agtaggtggt ttaaaggcag tatctaaaga tgctgaacgt aatatagaaa aaatgggaat
     4801 gaaagtagaa agaataagag ggaaagacag atatgataca tctttaaaga tagctagaga
     4861 aatgtataga acagtaggat ttgatgaagc atttttactt agctcaacaa caggattaga
     4921 gaacgccata tctgtatatt cttatgccgc taaaagtgga atgccaataa tatgggctaa
     4981 agatgaaggg tttgaggaac aaattgattt cttaaaaggc aaaaatctta aaaaaatata
     5041 tgcattaggt gattcaaaag agtttattgc agagattgat tctaacttaa aaaatataga
     5101 aggaataaag caaataaata agtcaagtac aaatgttgat ttaataaaga agttttatga
     5161 tgaaaaagat ataaagaaaa tctatactgc tagattagac tttggtagta gatctgatgt
     5221 aaacgaatat atttctttgg gagtagtatc agcaaaagaa aatatgccaa tactgatttg
     5281 tagtgataac ttaagtcgtg cacaagataa attcttgaaa gatagcaata taaatgatgt
     5341 agttgaagtt gggtatacag taggtgatta tagtctattt aagagcatat ttaatctaac
     5401 attcttatct tgtatagtat taatactatt attactattg attacattta gggcacttag
     5461 atatgagtcg aaatagtttt tgatttataa ggaagctttt cataggttaa atttacttaa
     5521 ttagaataat gtttataagc agttttaaaa gttctacttt tagagtttaa attagataag
     5581 gagaaagaag atgtcaaaaa cagctaaagc agcattgtgg attatggcag ccactatgtt
     5641 ttcaaaagta ttaggtttcc ttagagaact tgtacttgca aatttctatg ggacaggtat
     5701 gtatgcagac gtatttgtat taacattaaa tatacctggt ttaatcatag cagtcatagg
     5761 ctctgcagtt gctacaactt atatacctat gtactttgag acaaagaaaa gactcggaga
     5821 tgaaggtgcc ttaaaattta ctaataatgt tttaaatata tgctatataa tggctatagt
     5881 aatagctatt attggtcttt tatttacaga gcaatttgtt acagtatttg cagcaggatt
     5941 tagaaacgac cctgctaagt tccaagcagc aatattattt actaaaataa tgatttcagg
     6001 agttttattc ctgagtggaa gtaagatatt tagttcgttt ttacaggtga atgacagttt
     6061 tgtaattcct gggcttatag gaatcccata taatattata ataatagcag caatagcact
     6121 aagtgcaggt aaaaatgtat ggatactgcc agcaggagca ctattggcta tggcaagtca
     6181 gctattgttt caactgccat ttgcgtttaa gaagtcctac aagtataagc catatataaa
     6241 tttaaaggat gagtccataa aagagttagt caacctagta ttgcctatgt tggtaggagt
     6301 agctgttgga cagttaaata tatttgttga ccgattattg gcaaccactt tgggtgatgg
     6361 aaagttgtct gctcttaact atgcaaatag gctaaatgag tttgttatgg cattatttgt
     6421 tacatctata ataacagtta tatatcctaa actagcaaaa atgtctggta aagataataa
     6481 agaaggattt ataagtacta ttgtaaaatc ttcaaattgt attatattgg tggtgcttcc
     6541 tatatctata ggagctatta tattagcaga gcctcttgtt agaatattat tccaaagggg
     6601 taaatttgat gcgttatcaa cagaccttac atctatagcg cttagattat attcactagg
     6661 tttattggct tgtggagtaa gagatgtctt atatagggca ttttactctc tttcagatac
     6721 aaaaacgcca atgataaatg gaagtatagc tttaataata aatatagttc ttaatttaat
     6781 attgataaga ccattgggtc atgcaggtat tgctatatca actagtacat caaacataat
     6841 tacagttata ttattattta tatctcttaa aaagaaaaat ggatattttg gaggggataa
     6901 aattataaag actggtctaa aaagtttggt agcatctggt gtgatggcag tagctacttt
     6961 attgatatat aataatctat atgcatttat gggttcagga acaattaaag aaattatatc
     7021 agttggagct ggagtattag gtggagcttc tgtatatact gtattgatag ttttatttaa
     7081 ggtagaagag atggatttag cttttgaatt tttaagaaaa ggaaaacaaa aactacttag
     7141 aagatagata attaccttga aattttaaat tagagtcaga aaggatgaaa atatggatta
     7201 taagaataat tatgagatgt ggttaaattc tccttatttt gatgaacaaa cgaaaaatga
     7261 gctattaagt ataaaagatg atgaaaaaga aatacaagat agattttata aaaatttaga
     7321 gtttggaaca ggtggtttaa gaggtataat aggagcggga actaatagaa tcaatattta
     7381 tactgttaga cgtgcgacac tgggcgtttt aaattatatc atgaaaactc aaggagaaga
     7441 aggtaagcaa aagggaatag ttatagcaca tgatagtaga tacatgtcta gggagttttg
     7501 tatagaagta gcaaagactt taagtgctta tggtgtaaag gcttatattt ttgaagaatt
     7561 aaagcctaca ccagaacttt cttttgctgt tagatattta aagtgtgcta tgggaatagt
     7621 aataacagca agtcataatc ctaaagaata caatggatat aaagtatatg actcagatgg
     7681 tggtcaaatc tgtatagata tggcaaatga tataatagca gaagttaata agattgatga
     7741 ttacagtact attaaaagta tagattttga agaagcttta tctaaaaatt taataactat
     7801 attagataat gaagtagatg atgagtttat aaaagctgtt aagaagcaag ttttaagaca
     7861 aaatataata gatgaatacg ggaaaaaatt aaaaataata tatacaccta ttcatggtac
     7921 aggaaataag ccagtaagaa aagtcttaaa tgaatgtggc tttgaaaatg taatggtagt
     7981 taaggagcaa gaattgccag attctaattt ttcaacagta aagtatccta atcctgaaga
     8041 aaagtcagtg tttaatatag ctattgaaat ggcaaagaac aatggcactg atttgataat
     8101 aggaacagac cctgattgtg atagagtagg tatcgttgta aaagattcaa gtggtgaata
     8161 tgtagtctta aatggaaatc aagtcggttc acttttagtt agatatatat tagagagttt
     8221 agttgaagaa aataaacttc ctaaaaacaa tcctacaata ataaaaacta tagttacatc
     8281 agaactaggt gcaaaaatag caaaggctta taatgtagat tgtttaaata ctttaacagg
     8341 atttaaattt ataggagaaa aaataaaagc gtttgaagaa agcaatgata gaagttttat
     8401 aatgggatat gaggaaagct atggttactt aattggaact catgctagag ataaagatgg
     8461 tgttgtatct tcacttatga tatgtgaaat ggcagcatac tattcttcaa aaggtatgaa
     8521 cttgtatgaa gctttgatag atacatataa taaatttgga tactacaaag aggacttaaa
     8581 gtcagtaact ttaaaaggca tagatggaat aaaaaaaata aaagagatga tgctttactt
     8641 tagaagtgtt aaaatagata atgtggctga tgtaaaagta gataaaattt tagactataa
     8701 agatggtgtt gatgatttac ctaagtcaga tgtacttaaa tttttacttg aagatggctc
     8761 ttggatagct ataagacctt caggtacaga acctaaaatt aaattttact ttggagcaaa
     8821 ttcagacaat caagaagatg tagaatttaa gttgaacaat ttaatatctt atatattaaa
     8881 tgtagtggat tctatataaa aagctaaggg gagataaata atgaaagtat acaatgtcat
     8941 aatggctgga ggaggaggaa ctcgtttctg gccacttagt agacaagaag tacctaaaca
     9001 gcttataaat ttaagtggag aagatgcttt aataaacgaa actataaaca gaatagattc
     9061 tctagcaaaa aaagatgatt tatttatagt aaccaatgag aagcaattgg aagctttaaa
     9121 ggatatagta aaagataaat gtttggatag taatatattg ccagagccat gtgccagaaa
     9181 tacagcagca gctataggtt ttgcagcatt taacataatg aaaaaatatg gtgatggtgt
     9241 tatgtgtgta tatccagctg accattatat aaaagatgaa aaagaattta aatctatttt
     9301 ggaaaaagct atctatatag cagaaaataa tgataaacta gtaactattg ggataactcc
     9361 tacatttcca tcaacaggat atggatacat aaattttaat agagaaaata ctatagaaga
     9421 tgttgcatat gaggtagtgg agtttgtaga aaaaccaaac tatgaaattg ctaaagaata
     9481 tgtaaactct aaaaagtatg tatggaatag tggaatgttc gtatggaagg tatctaaaat
     9541 actagaagat tttaagagat acttgccaaa agtatatgaa aaattagaag atataagtaa
     9601 atacttagga acaaaagaag aaatggaaaa aataaaagaa atatacccaa ctatacaatc
     9661 aatctctata gattatggta ttatggaaag aagtaatgat gttatagtag ttcctggtga
     9721 ttttggatgg aatgatgttg gttcttggga ttctctaggt gcaatatatc caactgatga
     9781 tgaaggtaat atcaaaagag gtgaaaatat tactatagat actaaaaatt caattatata
     9841 ttcagatgac aaattaatat caacaatagg tattagtgat ttgatagtag tatctacaaa
     9901 tgatgcagtt atggtttgta gaaaagataa agctcaagat gtcaaaaaaa tagtagagca
     9961 gttaaaagaa gaagatagac aagaatatat gtaggaggtg ttcatttgat tcctaaaaaa
    10021 atacattatg tatggtttgg tggtccaaaa ggaaatatag aaaatatatg tataaattct
    10081 tggaaagaaa agcttccaga atatgagata gtagagtgga atgaaaaaaa ctttgatata
    10141 gagaaagaaa taaaaggaaa taagtttttg gaagaatgtt ataaaagaaa actttgggca
    10201 ttcatatctg attatacaag aataaaagta ctctatgaac aaggtggagt ttatatggat
    10261 acagatatgc aaatattaaa ggatataact cctcttttag aaaataatag gctaatctgt
    10321 gggtatgaag atgatagaga gtatataaat ggtgctataa taggtgtaga aaaaggacat
    10381 ccttttttaa aagacttatt ggagtattat gaaaaagaag tacttacttc ttcattgttt
    10441 acaataccaa agataatgac tcatcttatg gaaaagaatt ataagaagat tgacccaaat
    10501 aattatgaag aaggaatacg tgtttacgac aaagaatatt tttatccttt tggatttaaa
    10561 gaagacttta caccagaatg tataacagaa aacacttttg ggatacattg gtggggaaaa
    10621 agttgggcta aaaagagaaa ttatttctta gaaagcaaac acttaacagg tgtaaataag
    10681 atatggaagt gttgtaaaat atttgcaagt aatactttac gaagctaaaa gggagaaagt
    10741 aaaatgaaga ctttaaatga tgatagaaat attatgatta gaaaatatct ttatatattt
    10801 gttttgttta tcatattact tagaaagtat atgatatcat ttattgaccc aaatatggat
    10861 atagggatga ttaaatcagc gttattttat tcttcaatag gaatactaat gctacttttt
    10921 ttatttgaca agagaaaaag tataacagaa atggtactag ttggagtttg tgtcttactt
    10981 tatttattaa atagggaagg agctatatta ttaatcgttt tattagctgt atcagctaag
    11041 caaatagatg ataaatttat agttaaaaat tatttaataa tatcagcatg ttttttaatg
    11101 gtagctatat tattatttaa tctatttcct tcattgatat ttaatcagga agtccactat
    11161 agatatatag aaaaaataga tatgcttgtt acaagaatgg attttggact tggtaatcca
    11221 aatagtgtat tttatcatat ggttactata tatgcagcat atatattctt aagatataaa
    11281 gattataata aatgggatag aataatttta tttggttctg cattttttgt ttatcaaacc
    11341 acatatagta gaactggatt tttcactata ttagcaggat taatatttgt agaaataata
    11401 agatggatag atataaaaaa aataaaagga ctaccaatgc tcctaaagac attaccgata
    11461 atacttactt tatttagtgt aattattggt acagtatttg ataaaagcac tttattaaat
    11521 agattattag caagtagacc taagttctgg catgtatact tggctgaaaa ggggaatttt
    11581 ctaaaacctt ttggaaattc atatagtcct gctataaaag caactaatcc attagatagt
    11641 tcttatgtat atataattag tgtactagga gtagttgctt gtatattatt tatgtatttg
    11701 atgtataaag gaatagaaag ctttatagaa aaagataaaa aagcatattt agtagcttta
    11761 tttatatttt tactatactc ttttgcagaa aatttattat tagaagcttc atttagtttt
    11821 gcagtagtat tactaataaa agaagtgata ttaaatgata aaagggaaat agatttgtgg
    11881 aagatgaaaa gtaggaggta gtaatgttaa tatcattaat aatgccaaca ttaaatagat
    11941 atgatgatat atacttactt atggacagct tagaaaatca aacttacaaa aactttgaac
    12001 ttatagtagt agaccaaaat gataatagca aagttaaaga aatagttgat aaatacattg
    12061 ataagttaga tataaagtat ataaaaagct ctaaaaaagg attgagttat aacagaaatg
    12121 taggcataga caatgctgta gggcaaatta taggtttccc tgatgatgat tgtgtctacg
    12181 aaaatgatac attagaaaaa gttataaatt tctttaataa aaataaagat tataaaatat
    12241 acagttgcaa aactatggat tcaaataaag ttgatgcatt taagaaaatg tatgatggaa
    12301 cttgtgatat cacaagttcc aacgttttgg atactataac atccataact ttttttattg
    12361 attttgaagg taaagactat acaagatttg atgagaaatt gggtgttggt ggagagtttg
    12421 gtgctggtga agagatagat tatgttttaa acttattgag tttaggtttt aaaggaaaat
    12481 actttggaaa tgatataatt taccatcctg caaaaaaaca ttctaaatct aaagaaaagt
    12541 atcaaaaaga ttataactat ggaagaggct ttggagcgct ttgcaaaaag gaaatagtct
    12601 atagaaaaaa ttataagttt gcaaaagtta tggtatcaaa acttgtaaga aatataggag
    12661 gtcttatatt gagttccaat agagattatc atagtgccac tataaaagga agaattaatg
    12721 gatttagaca gtacaagtta taatgaggat ggtgtttatg gacaaggtta aatttttaat
    12781 aaatatgctt ttggcttttt ttatatatcc atttaataag cataagttta atggtagaga
    12841 tatctggctt ataggtggac attcagggga tatatacaat gataattcaa agttttttta
    12901 tgagtatatg ctaaaagaac ataatgatgt agaaacttat tgggtagtaa ataaagacag
    12961 taaagtattt gataaaattc caggcaaaaa attaataaga ggaagtgtag aaaactatct
    13021 gtattattac aattcaaaag ctatagtatt ttcacatgca ccatcagcag atatagcacc
    13081 atacaatttt gctgtacctg ttcttaatta ttttcataaa aaaacgataa aagtgttttt
    13141 aaatcatggg actataagtt ttaaaaaaag aaaacctatg aacaaaaagt tcaaaaatat
    13201 tatagataac ctatataaaa gctataatat agttacagca agttctgaat ttgaaagaaa
    13261 tgttatggta aatgattggg gaatgttaga tgactcagtt tatataattg gaaatgcaag
    13321 atatgacaat ttaccaacaa atgaagttgc acaaactcga gatatattat atactcctac
    13381 atggagagat tggataaaat ttagttcagg aaaatttacg gatacagatt attttaaaaa
    13441 tataatgaat tttttaaatg atgacaaatt aaataaaata ttagatgaga aagatataaa
    13501 tgtaaaaatt tatatgcatc atcttatgca tgagtttata gatgatataa aagaaaatat
    13561 aacaggaaaa cgaattgtgt ttttagataa aggggtaact ctagcaaatg aaatcagaaa
    13621 atcagctgca aatataactg attattcaag tgtagccata gactttttgt atatgaatag
    13681 acctattttg ttttaccaat tcgatttgga tgaatatatg gaaaaagttg attcatatat
    13741 agatttaaaa agtgaaatgt ttgggtcttt ggcttataat aatgatgaag ccgtgaataa
    13801 acttattgat attattgaaa ataattttga ggttatggat aaccaaaaaa atgaaagaaa
    13861 taagtttttc agatataatg ataataagaa ctgcaagagg atatatgatt gtgtattaag
    13921 taaaattaaa taaatatttt ataaatgatg aggaaggtaa tatgaagaag aatttagtat
    13981 ctataattac tcccatgtat aattctgaaa aatttattga agcaaccata aaatcagtat
    14041 taaaccaaac ttatcaagaa tgggaaatgt taattattga tgattgctca acagataata
    14101 gtcctaatat agtcaaatct tatatgcaac aggatagtag aataaaatgt ataaagactg
    14161 agactaataa gggtgtctct aatgctagaa atttagcact aagtaaggca acaggacaat
    14221 ttatagcttt tttagatagt gatgaccaat ggaatagtag taagttagaa aaacaagtaa
    14281 attttatgtt agaaaatgac tatgtaattt catttacttc atatgaactg atggatgaaa
    14341 atgataaaaa attaaacaaa gtaataaaag ttccacctaa tgtagattat agaagattat
    14401 taaaaggaaa tatcttagga tgtcttactg ttgtgattga taagtctaaa ttggattttg
    14461 aaataagaat gagtggtgta agacatgaag attatgtttt atggttatcg atattgaaaa
    14521 aagggcatat tgctcatgga ataaatgaag ttctagcatt atatagaaaa tctagtaatt
    14581 ccctaagtgg taataaaata aaagctgcta tgtggacttg gaatatatat agaaatatag
    14641 aaaaaatacc tttatacaaa gctatatact actttattaa ttatggaata aacggaatca
    14701 aaaaaagtta gacagagagg aagatttaat taatgtataa atataaattt actgtattta
    14761 ctccgacgta taacagagct catttacttg aaaatttgta caatgattta aaagctcaaa
    14821 cttatgattt caatgatttt gaatggttga ttgttgatga tggaagtagc gacagtacta
    14881 aagagttagt tgaaaaattt atatcagaaa ataaattaaa tataagatat atatacaaag
    14941 aaaatggagg aaaacatact gcaataaatc taggtgtaaa aaatgcagat ggagagctat
    15001 ttttcattgt agatagtgat gatggtttaa ttaaagattc tcttgaaatt gctaatgatg
    15061 agtgggattc tttagagaat aaagaaggat ttagtggagt tgttggactt tgtgtatatc
    15121 caagtggaga tttgataggg acagaaatgc ctgaagataa gaaaatatgt cactttgcag
    15181 acctatattt taagtatggc gttaagggag ataaaagcat agtatttgta actgatgagc
    15241 ttataaaatt tccttttcca gaaagaaaag aagtgagatt tttaccagaa agtgtagtat
    15301 ggaatgaaat gtctaaatac tataaagtta tatgtgtaaa taaacctatg ataataagag
    15361 attatttgga tgatggtttg actaagaata tattaagtaa aaatgcatta agaggtagag
    15421 cattagagtt tttatattta ataaatcaaa atacatatcc attaagtaga tatccatata
    15481 tgtggataaa aaattacata aatttagcca gatactcatt gttgtctgat agtcattatt
    15541 ttggtgaatt aagaaaagta tctgataaat tgttgtattt agcattattc ccattaggat
    15601 attacaaata tattggacaa agaaaattag tatctaagta aacttgttgg tatagtactg
    15661 ctagttgcta tataaggaga gtaaaaatat gaatgagcct ttagtatcga taattactcc
    15721 tgtttataat tcagaagagt ttttatcaga gacgataaag tcaatccaaa atcaaactta
    15781 taaaaattgg cagcttttat tagtggatga ttgttctaag gacaattcaa gtgaaattat
    15841 aaaaagtttt cgaaaagaag atgctagaat aaaatatata aagcttgaaa aaaattctgg
    15901 agctgcagta tctagaaatg ttggtattaa gaatgcggaa ggtcgattta ttgcatttgt
    15961 agatagtgat gatttatggg atagtagaaa attagagatt cagatagaat atatgttaaa
    16021 agaaaatgta ggtttttctt ttacatctta tagatatatg agacaagatg ggtcaaaaac
    16081 gaataaggtt gcaagagctc ctaagaaaat tgactatgaa ggattactta gaaatactat
    16141 aataggatgt tctacagtgg tcattgataa ggagattgta ggagagttca gcatgccttt
    16201 ggtaagaaga ggacaagata ctgcaacctg gttacagtta cttaaaaagg aaaaatatgc
    16261 atatgggatt caagaagatt tagtgaatta cagattagtt ggaaattcta tatcaagtaa
    16321 caagataaaa gcattaaaac gaacttggaa tacttataga aatgttgaaa atttaagttt
    16381 accaaaaagt ctatatgtat tttgtttcta tgtttttaat gccattaaaa aaagagtata
    16441 agttttatat acttggaggt aaaaaaatga aaatagctat agctgggacg ggatatgtag
    16501 gtttggttac aggtgtttgt ctagcagagg taggcaatga aaatgtagtc tgtgttgatg
    16561 tagatgaaca aaaagttaaa tcaatgaaac ttggaatagc acctatatat gaagaaggcc
    16621 tagaagaact tatggttaaa aattacaaca gaggaagaat agattacact acagactata
    16681 aaaaagctta tgaagatgta gatgtaatat taatagctgt aggtacacct gagagaagtg
    16741 atggttcagc caacctagat tatataaagg cagttgcaaa gcaaatagct gaaactgtga
    16801 caaaagatac tctagtagta ataaaatcta cagttccaat tggtacaaat gaagagatag
    16861 aagattatat aaatagaaac ctaaaaaatg atgtaaaagt agaacttgca tctaatccag
    16921 agtttttatc acaaggaaca gcagtaaaag atactctaca tgcttcaaga atcgttatag
    16981 gtgtagaaag tgaatgggct agagaaatat tagaggaaat ctataagcct tttaatcaac
    17041 ctataatagt tacaaataga aatagtgctg agatgataaa atacgcatca aataacttct
    17101 tagctcttaa aatatcattt atgaatgata ttgcaaatct ttgtgaaata gcaggagcta
    17161 acatagaaga tgtagcttta ggaatgagtt atgatgatag aataggaagt aagtttttaa
    17221 aggcgggaat aggatatggt ggttcttgct ttccaaaaga cacaaaagct cttcactggt
    17281 tatctgaaca taatggttat gaattaaaaa cagtaaaagc tgcaatagaa gttaatgaaa
    17341 gtcaaaaact taaattagtt agaaaggcat ctaagttagt agagagcttt caagatatga
    17401 aggttgctgt tttaggtttg acttttaagc cagacactga tgatataaga gaagcaccat
    17461 ctataccaaa tataaaatat ttgctcgaaa gaggggcaag agttactgta tacgacccaa
    17521 ttggtgtaaa taatactaag aaaatatttg gtgactctat actttatgct gattctgtag
    17581 atgatgcgat agaagatgca gagatatgtt ttataatgac agaatggaaa gatgtattaa
    17641 attatgatat acataagtat aaggaaaaga tgaaaaatcc tttagtcctt gatggaagaa
    17701 attgttataa aattaaagat atggaagagt taggaattga gtattattcg ataggaaggt
    17761 aaatattatg aagactgttt gttatttagc tgatgggtca aatcctcata ctttaaaatg
    17821 gtgtagtttt tttaaaaata aaggatatga cattcatgta atctctttaa atggaggtca
    17881 catggaagga ataaaaattt ataattttgg ttctaatgta gaagaactaa aaaatgaaaa
    17941 tatttctaaa aaaacgggat acttaggttc aataaagcag ataaagaagc tagtaaatga
    18001 aataaagcca gatatattgc atgcacagta tgcaagtagt tatggattta taggaagtct
    18061 acttgggtat catccatata ttatttctgt ttggggtact gatatttatg atttccctaa
    18121 taatggtttt atacaaaaac aaattataaa acataacttt agaaaagcag actatatctt
    18181 ttcaaatagt agagatatgg ctaaagaggc taataaatat acagctaaac atgtagatgt
    18241 aacctttttt ggagtagata tggataggtt taagcctatg gaagtagaaa aagaagacgc
    18301 ttttgtaata ggaattataa agagtttaga aaaaaaatat ggaatagaat atttaataca
    18361 agcatttaag atgcttaaag atgaatataa agataagaaa ataattctta aaataggtgg
    18421 ctctggaagt cagatggaca acctaataaa tttagctaaa gaattgggta tagaaaatga
    18481 tgttcagttt ttaggaagaa tatctccaga aaatgtaagc aagactttta attcttttga
    18541 tgtaactgta tttccttctt taagagaggg atttggagtg gcagctatag aatcagaagc
    18601 atgtgaagta cctgttatag ttacaaatgt aggaggacat ccagaatctg tatgggaaaa
    18661 tgaaactggc ttaattgtag aaccaaaaca accagaagaa ataaaaaatg ctataataaa
    18721 actaatggaa aatgatgaac ttagacttaa tatgggtaag aaagggcgtc aatttgttag
    18781 agaaaattat gaggttaatt taaactttaa tgatatagaa aaaatttatg attctatatt
    18841 tgataaatat aaaaaataaa atgaaaaaat acagcaaaag atagatgttg ttggctatgc
    18901 ttaggtgcgt agcttttgct gtttaaagga gggcttctag ttatgcgaga aagtatagat
    18961 ttagagaaag aaagcttttt taataggctt tttaaaaaat ataaatttag acatttaaca
    19021 ctattaattc tagatatttg tagcgtacta gtagcgtttc gaatgtcttt aagcttgaca
    19081 ggtaatttaa atgcatttac cactaatgat ataataattt cagcgtgtat atatatagta
    19141 cttcatatag tatcatttag attgtttaag tgctacaaca cattatggag atatgcagga
    19201 gaagaagaaa ttatatctat ttttgtggca actttagctt atttaattcc gatttatata
    19261 ataaataaat tattagggtt tgattatcct ataatgtttt atgtattaaa tacaatattt
    19321 attataatgt ttacaagtgg cgcaagaata gcctatagag ctataagaat agtcatgaac
    19381 aagacatatt ctagaggaaa ggtttcaaat atccttataa ttggtgcagg agatgcagga
    19441 gaaatggtta tacaggaact aaaaagaaat ccagaactta aaaaagtagc agtggctata
    19501 attgatgatg ataaaaataa aattggaaga ataatccaca atgtgaaaat tgtaggaaca
    19561 acttcagata taaaagatgt ggtagaaaag tacaatgtag atgagataat cttttcaata
    19621 gcaaacatag agaaacgtag aaaaaaagag attattgata tatgtaaaaa cactaactgt
    19681 aaaataaaga caataccagg aatttacgaa attatagatg gaaaagttga tataaaacag
    19741 attagagaag tagagataga agacttgttg ggtagagagc ctataaaaac aaatttaaga
    19801 gagataagta attacattga gggaaaagta atcctaataa ctggaggagg aggctcaatt
    19861 ggttcagagc tttgcagaca gattgcagga tttaatccta aagagcttat tatagttgat
    19921 aattatgaaa ataatgctta ctcaatacaa caagaattgc ttagaaaata taaaaataaa
    19981 ttagatttaa aaacagtaat agcatcaata agagaagaaa aaagaatgga tgaaatattt
    20041 aataaatata aaccagaagt ggtatttcat gcagcagcac ataaacatgt tcctcttatg
    20101 gaatcgagcc ctggcgaagc tataaagaat aatatatttg gaactttaaa catagctggc
    20161 ttgtcttcaa aatatagagc taagaaattt gtccttatat ctacagataa ggctgtaaac
    20221 cctactaaca taatgggagc tactaaaaga gctgctgaaa tgattataca gactatgaat
    20281 gctgaaagcc agactgaatt tgttgctgtt agatttggaa atgtgcttgg tagtaatggt
    20341 agtgtaattc cactttttaa gaagcaaatt gaagatggcg gaccagtgac agttactcat
    20401 ccagatatta ttagatattt tatgacaata ccagaagcag taggcttagt tatacaagct
    20461 ggggctatgg ctaaaggtgg agaaatattt gtacttgata tgggagaacc tgtaagaata
    20521 ttggatttag caaaaaactt aattaaattc agtggatttg agccagatgt tgatataaaa
    20581 attgaatttt ctggtcttag accaggagaa aagttgtatg aagaactact tatgtcagaa
    20641 gaaggtctgt tggatacaga gcataaaaag atttttattg gtagaccaat agatgttgat
    20701 agagaaaaaa taacaaaata cttaaaactt ttaagagaaa ttactaacaa tgaagaggtt
    20761 gaaaaaatag atggtattat gagggaatta gtgccaactt atattaaacc agaagatgct
    20821 aatatcaaag aaattgcaac aactagagaa aagtagaata tatttctaat ttgtaaccag
    20881 ggggagcaaa aagaaacaaa tatatactaa aaagaaacaa aagattacaa aaagattaca
    20941 aaaagattac aaaaaaggtt gcaatttatt ttgatgtaag atagaatgtt aattaggaat
    21001 tggaaacgaa ggaacaaaat gttaaatttt cgaaaggatt aggtatttga gattatgatg
    21061 aatattaaaa ataaaaaaca tatattgaaa aaatttatag ctatggtgtt aatagcagga
    21121 gttgtcacag tagaagcagg agcaataacg gctagtgcag cagagccaac taattcacct
    21181 atgagtgcaa cagtagacca gtgtgatttt ttaaatgtaa gaagtggagc tagtgcaaat
    21241 gatgcagtag taggaaagat aaacacagga gacaaagttg aagttttaga attacattca
    21301 aatggatgga ttaaaataaa aagtgttgac aatgttacag gatgggtaaa tggagattat
    21361 ttaactattc aaggtggaaa tgtagatgca aaggtacaaa atgttttaaa cttagcattt
    21421 aagcaacaag gtaagccata taaatgggga gcaactggac caaattcttt tgattgttct
    21481 gggttcactt cttatgtcta taaaaatgga gcaggagtta atttaccaag agtatcaaga
    21541 tcacaagcaa cagtaggaaa gaaagtatca agagcagaat taaaaccagg agatttagtt
    21601 ttctttggtt caggtggttc aataaatcac gttggattgt atgtaggaga ttctaaattc
    21661 atacattcac cacaaacagg agatgtagta aaagttacta gcatggctcc aggaactaat
    21721 tatgcaagaa gacttataac tgctacacgt gttttacaat aataatagat attgatttac
    21781 ataggattga atattaacat taaatatata attacattta atgttttaca tagagattta
    21841 agagtttagt aatattaaaa tagtgatgaa cgttataaaa acagcaaaga agataaattt
    21901 ctttgctgtt ttttttataa taaattattt catattataa tcaaatatca aatataaatt
    21961 ttaactatat tattcaatat ttgacagata atatgaattt tagatatact taataagaag
    22021 tatattgtag aaaaaataaa tttaaaaaat taagggggat gaaatgaaaa aaataagtat
    22081 attagtatta tcattgataa tgacacttac tatgtgtagt gtaagttcat ttgctgattc
    22141 atcaaatgat aaggaaatga gagctgcttg gatatctaca gtttataatt tagactggcc
    22201 taagactaag aataatgaag ccaagcaaaa aaaggaatac acagatttat tggacaaatt
    22261 aaaaagtgta ggaataaata cagcagtggt tcaagtaaga ccaaaatcag atgctttata
    22321 taaatcaaat ataaacccat ggtctgaata tcttaccggt acacaaggta aagaccctgg
    22381 atatgaccca ttaccatttt taatagaaga agcacataaa agaggaatgg aatttcatgc
    22441 atggtttaat ccatatagaa taactatggc agatgagagc atagataaat tgccagcaaa
    22501 ccatccagca aagaaaaatc caagctgggt agttaaacat ggaaataagt attactacga
    22561 cccaggttta ccagaagtta gaaagtacat agtggatagt atagcagaag tagttcaaaa
    22621 ttacgatata gatggagttc attttgatga ttacttctat ccaggagtaa gtttcaatga
    22681 tactgcaaca taccaaaaat atggaaaagg acaaaataag gatgattgga gaagagaaaa
    22741 tgtaaatact ctacttagag atgtaaaagc gtctataaag agtataaagc ctaatgttgt
    22801 atttggtgta agcccagcag gtatatggag aaataagagt agtgacccaa caggttcaga
    22861 tacatcaggt aatgaaagtt atgtaggtac atatgcagat acaagagcat ggataaaaca
    22921 aggattgatt gactatgtag ttccacaact ttactggcca attggtctta aagcagcaga
    22981 ttattctaaa ttagtggcat ggtgggctaa tgaagtaaaa ggaacaaatg tagacttata
    23041 cataggacaa ggtatatata agcaaggtca atcgagttat ggtggacaaa atatagcaaa
    23101 agaaatagta caacaagtaa cattaaatag aaagtatagt gaaataaaag gtagtatgta
    23161 tttctcagct aaagatatag caaatagtac ttctatacaa aaggatttaa agagtttata
    23221 ttctagtagt gaagaaccag ttactccacc atctaatgtt aaagtggaga aattaagagg
    23281 cgacgaaaga tatgacacag cagtagcaat aagtaaaaaa ggttgggcta caaattcaga
    23341 tacagtagta ttagtaaatg ggtactctat agttgatggt ataacatcaa caccacttgc
    23401 tacatcaaat gatgctccta tactattggt aaataaggat aatataccta catccactaa
    23461 gaatgagtta aaaagactta atccaagtaa agttatttta attggaggaa ataactctat
    23521 tggagataaa gttgagagtg agataaaaga tacattgtca aatgtatcaa taaatagagt
    23581 tggtggttca gatagatata gtacatcttt aatgatagca aaagaattag taaaaactaa
    23641 tccagtagaa aaactatata taacaagtgg tacaggtgaa gcagattctc tttctatagc
    23701 ttcaaaagca ggagaagaaa agcaaccaat agtattagta tcaaaagata atgtaagtga
    23761 tgaagtttac aattggatat cagatttgaa agtaaaagat gcttatttta taggtggtaa
    23821 tctaagtata tctgattcag taataaataa gctggataag gtcattacaa atgatgtatc
    23881 taaaaataga atagctggag aaaatagaga agaaacaaat ggaaaagtaa tacaaaaatt
    23941 ctatccaaat gctgaatatt catcaatgtt tgtaagtaaa agtaatcaat tggtagatgc
    24001 attaacatca ggtcctttag cagctaaatt aaaatcacca gtagttatgc ttggaaatag
    24061 tgtaacatct gctcagaaaa ctgctttaga gcctaagaaa actactctag tatacgaagc
    24121 tggagatggt ataaatcaaa acacattaaa cactttctta aacttagtta agtaataaaa
    24181 aagttaattt aaaatacaga gtattttata aatgtagatt aaaatgaaaa ttatcatgat
    24241 aaattagaaa ataaaaaaga actatatgca ttttgtaatc aagtgtacac agcaacttga
    24301 aaataattat gcgatatagt tcttttttag taattaatct tatacttttt aataaacttt
    24361 atgttgaatt ttttattgtt taaactctat gtcattaaat ataaaatcgt gtaatatgtc
    24421 taaagtatct gggtcaaatc taagaaccca tccagctttt ccatatatac cacctgtact
    24481 atgaatttca tcatcaatag gaaattcaaa ttgcttaata tttaagtcgc ccatttttaa
    24541 aacttgagct cctaaagaaa gtattgcatt aggcttcatg ttagttttaa catatggaag
    24601 aactgcatct aataaacctg gatatttagt aacaggtaaa gtttccgttt tcttgattat
    24661 agcttgtata atctgtctct gtctgccatc tctagcaaag gctgtatcgt tgtgtctgat
    24721 acgtgcaaaa gatagtgcct gatatccatt tagagtctgc tttccagagt ttctaatgta
    24781 ttgtatgcta cctttatcat cacttttatt ccatttataa gtttcaggaa taaatttatt
    24841 tagttctcgt atttctcctt tttcaacagt aacctcaacg ccaccaatag catcaattat
    24901 atacataaat gattcgaaat taacacaagc ataattttga atatcaatat taaaattctc
    24961 ttcaattgtt tgtattaata aatcagcttg tccataagca taagcatgag taagtttttg
    25021 tttaccatga ccagggatgt ctacatagct atcacgagct aatgatgtaa gtttgatatc
    25081 attgtgtttg ttatcaatag taagtatcat catagcatct gaccttgaag aatcttcatt
    25141 tggtctagca tctgttccca taagtagtat gttaattatc ccatcttcat ttttatggtc
    25201 attgttatcc aataaatcac tacttatgct cgagtcatga atagcagata atttataata
    25261 aaaatatcca tagacactta tagggaatat tactaccaag aaagccagta gtataacaaa
    25321 tttctttaat tttgacaaaa gtaccccttc tttcttcttt ttcctgtata ttttttaata
    25381 gcatacactt aattatatta aagtattgag atatattcaa tacttgtttc agttacaaaa
    25441 aaaagacaat gatataattt ataagaaaat ttatcgacct atatatatag ttaaaagtat
    25501 atataatata ttttttaaaa gtaatttata ttttaaaata aaaacacacc ccataaatac
    25561 aaagtaattt gcatttaaga ggtatgtatt tatcatcaaa ttattggttt gttttggaat
    25621 ttctaatctt caaccataat atctttaaat atgaaatcat gcattgcatc tagttcatat
    25681 tcctcaaaag gtattacata gcctgcatta cctatttttc taccattttc agtagaaaga
    25741 ggaaactcca tagatttcaa atttaaattt cctatactta aaagttcttt tcctaaagat
    25801 attatttcat taggattcat attagttttt acatacggaa gtattgtgtt aactagatta
    25861 ggatatttag tgactggtaa atccttaaca ccatttatta aactctgtat aacacttctt
    25921 tgtcttctat ctctttcatc tgtactatca ttttttctta tacgagaaaa tgcaagtgtc
    25981 tgatatccat ttaatttttg ctcaccagaa tttcttatat atttaagaga acctttattg
    26041 tcatttttat tccatttata agtttcagga ataaatttat ttaattctct tatttcactc
    26101 ttatcaacag taacagtaac tccacctaaa gcatcaacta tatccataaa agagaaaaag
    26161 tcaactacgg cgtagttttg tatatcaagt tggaagtttt cttctatagt ttctattaat
    26221 aagttagctt gtccatatac gtaggcatgt gttaattttt gttctccacg tcctggtata
    26281 ttaacataag tatctctatt taatgaagtt agcttaagac ttttattttt attatcaata
    26341 gtaagtatca tcatggcatc agaacgagaa acttcttctc ctggtcttgc atctgtacct
    26401 aacaataata tgtttgtgat attttcttcg tttttatagt cattactact aagcgcctct
    26461 gtatttatac tatcatcttg tatagttttt aatttagtaa aaacatatcc atatatacta
    26521 acaggaaatg ctataactaa aagggctaga aatataacga actttttgag tcctgacaat
    26581 aatatcccct actttctaaa ttttttaatt acacatagtt aattatatta aaaatttgtt
    26641 acataagcaa taaaaaacac aataaattag cataagtttg tcataatata attattttta
    26701 tagcatttta tagaagaaag tgtgtagtta ttattgattt tattggaata taattgtatg
    26761 aagacttaaa tttttataaa ctgatgtgta tattgaaaaa aatattttcc tgaatgtagt
    26821 tatagcatct tatgaaaggt gcaaattgta atttaaaatt tttagtgtat aataagaatt
    26881 gaaaattcaa accgataaaa attaataaaa aataattata aattaataat ttgggggagg
    26941 aaatgtatga taaaattaat agcaacagat ttagatggaa ctttgttaga tgaaaagagc
    27001 gaaataaatc cagagtttta taaagtattt aaaaaattaa gagagagagg gataatgttt
    27061 tctgctgcta gtggcaggca gtatcaaaat cttataaaaa aatttgaaga tataaaagat
    27121 gatatgatgt ttatatcaga aaatggaaca ctagtagtat ataaaggaaa agaaatatta
    27181 tcaaatcctt taaataaaga attagtaaat gaaatcatag agacaacaag aagtataaaa
    27241 gggaaaaaaa ttgtaatgag tgggaaaaaa tacgcttata tagaaagcaa agatgaagcg
    27301 tttatacaag aggtcagtac ttattatgca aagtttaaaa ttgtagaaga tttgacgaaa
    27361 gtagaaggag acatattgaa gatagctgta tttgacttca aaggtgcaga acataataat
    27421 aatatctact ttgaaaagtt ttctgataga gctcaagtat gtatttctgg agtggagtgg
    27481 cttgatttaa ctgcaaaagg tgcaaataaa gggagtgcca taaaaaaagt acaaaaaatg
    27541 ttagatataa aatatgaaga aactatggta tttggtgacc agttaaatga tgtggaaatg
    27601 atgaaaagtg cctatcatag ttatgccatg gaaaatgcaa atgaacattt gaaacaaatt
    27661 gctagattta gagcaaaaag aaatactgaa aatggcgttg tagataagat aaaagaggtt
    27721 ataaaaattg gatagtcata tgtatagatg aaaaagatta ttttgaagaa atcaaaaaaa
    27781 tatcaatgta attttgataa ataagtttat ttatgcgaaa attacattga tatttaataa
    27841 acatcaataa tagatattta tttttttatg atattcagta ttttgttttc tatttgagtt
    27901 gcattttcag cagaaattaa ataaacatat ctacctgcct tctcaattat agtattattt
    27961 gctatttcag aattgatttt ttctccatgt ccttctccaa aaggtgcttg tatcatatct
    28021 tttttataat tttctagagt tttatagatt ttatctacct cgtcagtttt tataacgata
    28081 atatcttcta gagataattt ttcagaagaa ttaattacaa aaccttgaat tatgtcttcc
    28141 tctacatcat taaaatagtt aaaatccttt gcatcaaaga tttttgattc ctttataagt
    28201 aaatcagaag attttaatgc tttttttatt tttgatgtag aaacgttttt gtaatcacca
    28261 cttgaagaac aactaaccat tcctaatgat aagattatcg aaaaaaacaa tattaaaatt
    28321 tttttcatga taaacctcca agttttttaa actctgtctt aaattagttt actacataat
    28381 tatataaaaa taaatgtatt taatgttaca gttttgatta ataatgtaat attttgataa
    28441 taatacctat tgagaggtga ttagaaatga gaataccaaa acatgtagga attataccgg
    28501 atggaaatag aaggtgggca agtcaaaatg gtatgacaaa agataaagga tatgaaagtg
    28561 gaatagaccc tggactagag tcttatagat tatgtagaga tttaggctgt gaagaggtga
    28621 ctttttatgg atttacaact gataatacaa gacgtccatc agtgcagaca aaagctttta
    28681 caaaagcatg tattgatgct gcaaatatat taatagatga aggtgctgct ttattagttg
    28741 taggaaatac aaaatctcca atgtttccag aagaattatt accatatact aatagaatta
    28801 aagccaaaga aggtcaaata aaagcaaatc ttctggtaaa ttatggttgg tattgggact
    28861 taaataatat atcaggagta gaaaatgtaa ataaaaataa tatacaggat tacatacagt
    28921 catttgatgt atccagacta gacttgataa tcagatgggg tggaagaagg agattaagtg
    28981 gtttccttcc tgtacaatca atttatgcag atttttatgt tatagataat tattggccag
    29041 attttaagag tgacgatata aaggacgcat ttagttggta tgatacacaa gatgtcactt
    29101 taggtggata attaaaaata ttctgattta aggttgtaga gtgttttaaa ttattgaaaa
    29161 aaaaacttct tgatggtaaa ataacactaa taatatgtaa aagtgtaaaa atttatacca
    29221 agaagggtga agaaatgtca aaaggcaata ataacaataa ttcaagaaac aaatctaaaa
    29281 agaccagtca tctgaataga aaaaagcgaa aattaaataa gaaaaagtta gcagtgttga
    29341 tttgctttac agttttattt ttatttattg catttaaggc tactcaaggt gtagtagctt
    29401 tggtaaaaag tatggataaa agtaataaaa ctaatcaaca acaaaatgtt aactcagaac
    29461 aatttgacct tggtaatgaa gaggaaaata aaaagaaaaa atatactgta tttatagacc
    29521 caggtcatgg aggcaatgat aaaggaactg aaagcaagac cagtaataga tatgaaaaag
    29581 acctaaatct acaaattgca aaaaaattag caaataaatt atctaaacaa aaggatattc
    29641 aagtagtagt atccagaact gatgatacat atatatcatt aaaggataga gctatcttag
    29701 caaataatag ttctgctgat gtgcttgttt ctattcattt aaatgcagag aaaaatggta
    29761 atacagcaac aggtatagaa acttggtata ggaataaagc tacagatggt tcaaaagaac
    29821 ttgcacagac agtacaaagt actatagtat cctatgttaa agttagagat aggggaatag
    29881 ttgaaaataa ctttgaagta cttagagaat caaatatgcc tgctatatta atagagtgtg
    29941 gttttttaac aacaccttca gaggaacaaa aaataataaa tgaaaaatat caagaccaac
    30001 ttgcggaagg aattgttcaa ggtgtacttt catacctaga ttctaaaggg aataagtaag
    30061 ttaactttac tttaatgtga ggataaattt aaaagtttaa ctgtgtaaat tgtattaact
    30121 ttatttcgta atgttgcaaa cattgtttga atttagaaac tatgaaatta gaaattattt
    30181 tatctatatc taatttcata gttttttttg caaaaaattt atagttataa aat
//
LOCUS       DAYAPA010000039        29978 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.039, whole genome shotgun sequence.
ACCESSION   DAYAPA010000039 DAYAPA010000000
VERSION     DAYAPA010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 29978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29978
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.039"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(814..1317)
                     /locus_tag="ACGI59_001624"
     CDS             complement(814..1317)
                     /locus_tag="ACGI59_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HFG9739413.1"
                     /translation="MFLFKIDDYIMYGMTGVCKVMDITNERFTNGIKKEYYVLSPIYS
                     NNTVIKIPVDNEKVPMRKLLSKINVLSLINDIPNMDTSWIDNEKLRSEQFKKILRGGK
                     CEELIKLVRSIDNNREYVKSIGKKTHQADDNIMKEAERLLNEEFATILDISPNEVSSY
                     ISSHIPQ"
     gene            complement(1534..2943)
                     /locus_tag="ACGI59_001625"
     CDS             complement(1534..2943)
                     /locus_tag="ACGI59_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFG9739414.1"
                     /translation="MENYFDKQAKELYNKASEIINTHSMLKANRANEKFDIDIPQDFK
                     YEFFSLVDKVNLSLMEEEDNFYGYFLFQMSREIRFDISSPTGVNFKGAKYIIYFNPII
                     FLTLDIKQMETTIKHEIHHILSMHLMRAKELKGKYSTLAINMAMDIVVNKFLNNLPPY
                     ATTLEWVNLKYSLKLEPYEPFEYYVEKIQTELDLMEVDEDGEEDDSDKYGDIEIDYSP
                     ERTHDIWDESNEIDENTLREFTEKFINSSQKGKVPAYLESMISSLKNSKGELPWNLYL
                     NRIMGTVENNKKKTITRRNRRQPNRLDLRGELRSHKSEIAVALDVSGSISDEEFKQAI
                     KEVLSIVKNYNHEITIIECDNEIRRVYKVRTEKDIKDRIRIGGGTRFSPVFEYANNKK
                     INLLVYFTDGKGENKLQVIPRGYKILWVISGRGDKISLKEPYGAVKKLTKVKTNDEIL
                     EINDVRNDGYSMNSQAPIL"
     gene            complement(2947..4077)
                     /locus_tag="ACGI59_001626"
     CDS             complement(2947..4077)
                     /locus_tag="ACGI59_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFG9739415.1"
                     /translation="MNFIDTLKSVDLILSTGEVPLIVGESGIGKTALANKLAKENDWS
                     LIVIDGNLLKEGEIGGLPTIESYVGVNSNGYKTEKKTTVYAVHNKLREIDEEISKSKT
                     VLLFIDEINRCEHTVQQELMNLILNREINGYKLHDDVKILAAMNPSSKYGSDFDYQVV
                     DMDAAQENRFVWLNMESDHTQWIKWAIDEGIERKVIEFISTFPEYLHKINEDDVRATP
                     RSYERVSKIYKVYKEKNNSIPRAVFLNVVKGNVGKVIAEEFISFIESNSEPLISYEDV
                     FLGESIDESIVERVKNESHTRLYLSAMNILKDLELNIKNDKYESNHYINRFIEFLKMY
                     PVDLMIGIMKDIRNSYIEVYKKAIENEEFVKSYFESYSLIRG"
     gene            complement(4089..4892)
                     /locus_tag="ACGI59_001627"
     CDS             complement(4089..4892)
                     /locus_tag="ACGI59_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9739416.1"
                     /translation="MEKLRVIEMIKNNPNIIATIDNPTDEMKLLAIKENGLVLEYINN
                     PTREMQELAIDNNIRAIKFIDNPTEDMMIKAVNEGWSILDYIKNPTDKVIEMAINQAG
                     WAIKYAKNPSEELQLLAVRKNYDSIKFIKQPYESVQEEAVRISYDALRYIDSPSYNAE
                     LIAIKNNEAAISFITDLDKNKMLEFLKVNILVIKYILKKVSKDELESALKEVLSKEDV
                     EEKYIRDFLNCSVIDRNSESIPIDKIMFIYKYGSKTAKKIAVDEKLKIM"
     gene            complement(5274..6155)
                     /gene="pepI"
                     /locus_tag="ACGI59_001628"
     CDS             complement(5274..6155)
                     /gene="pepI"
                     /locus_tag="ACGI59_001628"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline iminopeptidase"
                     /protein_id="HFG9739417.1"
                     /translation="MKITEGYMPFKGFKTYYRIVGENTEGKKPLVLLHGGPGSTHNYF
                     EVLDKIAESGRQVIMYDQIGCGNSFVEGHPELFNADTWIEELIELRKHLGLDEIHLLG
                     QSWGGMQAIWYAIEYKPKGIKSYILSSTLSSAKLWEKEQKRRISYMSEVDQKALLDAV
                     NTGDYSSKEYNDALERFMEMYCAGEVTEDSPECLRRPKKSGSEAYIVGWGQNEFSPTG
                     TLSGYEFTDRLHEIKEPCLVTSGAIDLCSPYIAKTMYDRIPNSKWELFEYSRHMPFVE
                     ENEKYIKVLTEWLNAND"
     gene            6611..7585
                     /locus_tag="ACGI59_001629"
     CDS             6611..7585
                     /locus_tag="ACGI59_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HFG9739418.1"
                     /translation="MNNINSLKVIKLSLGCSLAIFIAWLLKLEYSMVAGVIVLLTVKD
                     TKRETLKGSIGKIYGFLLCTIFSYLCFNILGYNLTSFSIFIFIIIPLCFLLNIQDVIA
                     MCVVIASHYYLQGETSIKWILNEAGIFAVGTGIGVLINMYIPTNIHKIHEVQKKLQEE
                     VSIVLIDIADIIVNPKKENGYSRDLYTLNSLIDSSISETYDNINNTLLSDTRFFLEHM
                     DIIKSQRDILENLYSYVSQLNSTPPQAHILSAFIHKIGYTEFEAETGNLLLEELKRLM
                     LSMKNQPLPVDRTEFENRAILFLCLTELKQFLVNRKHAQMLRDNNFYK"
     gene            complement(7701..8762)
                     /locus_tag="ACGI59_001630"
     CDS             complement(7701..8762)
                     /locus_tag="ACGI59_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HFG9739419.1"
                     /translation="MKKIRKLATKLLITTIVLISGMSMTVYGMTAKEVTAKTPKSYVT
                     GTNSVYGPKLSQAQLNSVAQATADFMNKKITKNMTTDAKILVAYNHIKNNTTYVDWNA
                     VEGANTAYTLVTKKGACSGMARSMKALCDAMGIESYYVHSTSNDHQWNLIRFGDGRLY
                     HVDIDANKSAGKDIIYKSLSHPLPFDKTAYPKVGAIVQTVKPEQTQKPAPPGKHTMLP
                     HQEGAKAVGNFGKKGEDINEKHVMYEGVRCSVVNSSVNTITFTGSESNLDPSNLTIAV
                     IGFMNTPYGMFALAPEGKPVEYPFKYDKPFSLDFSGKNRSGAELAKYCKKNDVTIKIE
                     ITDEKANIGLGQIMYVRYE"
     gene            complement(9374..10225)
                     /locus_tag="ACGI59_001631"
     CDS             complement(9374..10225)
                     /locus_tag="ACGI59_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFG9739420.1"
                     /translation="MSCILKLKQIYEDLTEVDKKIADYILNNTEAISKLSVSELASNS
                     KTSTASIVRFSRKMGYSGFGDLKIEIAKDLMGKENEYTYVDENYDCNIDSVINKITNK
                     NIETINQTRLLNEKDIIKEAVEQIIKAKNVYIFGVGGSALVALDLQMKLLRINKQAFT
                     SLDSHTQLMVSSNVDKEDIAIAISYSGESKEVIKSIENAKLKGCKVICITKYSDNHLS
                     KISDLKLVVPNIEKRLREGAISSRIAMLTLIDIIYISIIQENLNEAEQKLQETKRILD
                     YLNINNA"
     gene            complement(10242..11162)
                     /locus_tag="ACGI59_001632"
     CDS             complement(10242..11162)
                     /locus_tag="ACGI59_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HFG9739421.1"
                     /translation="MYFLGVDGGGTKTTFTLVDEELNIVGTITKGTCHYNQIGFDNLT
                     KLLITGLEEVCKDAKINVEEITYAFVGLAGYGKIKEVLYALEVATKNAYSHINYTLGN
                     DVEIALAGSLNGEKGINIIAGTGSIAQALDKDGNLHRCGGWGYVLGDEGSAYYIGMAT
                     LKMFTMQSDGRCSKTKLYDLIKRHLNIENDYDIIKYVNDEIKGDRIEIAKFATICLKA
                     VIEGDNTASKIFDDAAYELSRLIIGLEHHFEQGTKIKVSYSGGVFKSGDLILEPLKKY
                     LNKTRFGIVRPVLTPDLGACLLAKKKYYSK"
     gene            complement(11272..12186)
                     /gene="murQ"
                     /locus_tag="ACGI59_001633"
     CDS             complement(11272..12186)
                     /gene="murQ"
                     /locus_tag="ACGI59_001633"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFG9739422.1"
                     /translation="MLIKTLENLVTEGRNKNTLQIDKEDTLGIIELINNEDKTVAYAV
                     EEQKESIAKAVNIIVDRMKQGGRLFYIGAGTSGRIGILDATECPPTYGVDFELVQAII
                     AGGNQAIFKAIEGAEDDKELGKQDIIDRGVTSKDVICGIAASGRTPYVIGAMEYAKEL
                     GCAVLSITMNPNSEMSKKADLPINIIVGAEVIMGSTRMKSGTAQKMVCNMLTTASMVK
                     MGKVYSNLMVDVKTSNEKLVERAKRIIMIATNVKYDVAEKFLEEADNSVKLAIFMIKS
                     GLDKDSAKSILDRQEGYISEALKSIEKL"
     gene            complement(12285..13382)
                     /locus_tag="ACGI59_001634"
     CDS             complement(12285..13382)
                     /locus_tag="ACGI59_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFG9739423.1"
                     /translation="MGRLGISIYPEKTTSKEIYDYIDKAAENGFTRIFSCLLSVKDTK
                     ENIIKKFKSINEYAKFKGFEVILDVNPRVFGELEISYKDLNFFKKTKADGIRLDMGFT
                     GSEEALMTFNPQNLKIEINMSNNTNYIDTIMDYQPNKENLIGCHNFYPHRYSGLNLEH
                     FIKCTERFNKYGLRTAVFVTSQNETTFGPWPVTDGLPTIEMHRNLPIDIQVKHIIAMN
                     NINDIIISNCYPTYEELKVLGSMRKDMVTFDVAIEENIREVEKKILFEEIHFNRGDIS
                     DSLIRSTQSRVKYKGYKFELFNTPKIIKRGDIVIESSEYGHYAGELQIALTDMKNSGK
                     SNVVGYIRKEELFILDYIKPWQKFNFKPSTK"
     gene            complement(13615..14961)
                     /locus_tag="ACGI59_001635"
     CDS             complement(13615..14961)
                     /locus_tag="ACGI59_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFG9739424.1"
                     /translation="MLDKFTEFLDTKLSTSMARLAEQRHLRAVRDGIVASLPLIIVGS
                     FFLILAFPPLPDSWGVTQYLKENAVTILLPYRMTFFIISVYSTFGIGYSLAKSYKLDG
                     LTGGLLATATFLLTIVPTAIEGLTPDLLKIVEKSPELLAWYNSIPQGWQMPMANLGGG
                     SMFVAIVTSIIGVEIYRFTDKSKFKITMPEAVPASVTRSFEALTPAALIIILMAIVTY
                     FLGFDWHGFIAKLVTPLVSATDTLPSVIILIFLITFFWSFGIHGVSIIGSLARPLWIQ
                     LLDANTASYAAGQAVPGIAAEPFFQWFIWIGGSGATIGLALLLAFRAKSAYGKSLGKA
                     TVIPAIFNINEPIIFGAPIVLNPILIIPFVVAPIVMATVSWFAMSANLVSRVVVTAPW
                     TLPGPVGAYLATGGDWRASVLNLLCIAISIIIYYPFVKMYDKRLLHEEKVEEQEAY"
     gene            complement(15050..15358)
                     /locus_tag="ACGI59_001636"
     CDS             complement(15050..15358)
                     /locus_tag="ACGI59_001636"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFG9739425.1"
                     /translation="MRVLFVCSSGMSSAIAVNALQKEGAKNGIDIDVLAVGTQEFEDE
                     VKNGWDIAMVAPQVRHRFDYLKAFADEVSVPCALIQAQAYSPLGGPKLLKQVQELLSK
                     "
     gene            complement(15621..16019)
                     /locus_tag="ACGI59_001637"
     CDS             complement(15621..16019)
                     /locus_tag="ACGI59_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9739426.1"
                     /translation="MSNNKKKKRKKNNNYKNAKSTKSNKNTKKQMEKNQRTDYNIPTI
                     SFKSGLILIVSSLVSTVVFPLLLSIFGIDNRFGVVIGNSLITSFAVAYTRYFVETKRG
                     FSLGFFRLYILFALSFAIIGYFWIYKGIHI"
     gene            complement(16021..17172)
                     /gene="anmK"
                     /locus_tag="ACGI59_001638"
     CDS             complement(16021..17172)
                     /gene="anmK"
                     /locus_tag="ACGI59_001638"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase AnmK"
                     /protein_id="HFG9739427.1"
                     /translation="MYSVGLMSGTSLDGVDAVLAEISGNGRNTKVKQIEFITLEIPKD
                     IKDEIRKCCIEEESSVDLICSLNFKLGYLFSKAVKSVCHKANFHIANLDFIASHGQTI
                     FHIPRSYNNFVSSTLQIGEPAVIAYETNTKVISNFRVMDIAAGGEGAPLVPYSEFLLY
                     SDNNKNLALQNIGGIGNITIIPKSCNIDDVFAFDTGPGNMIIDGVCQRLFNRKYDKNG
                     YFASKGKINEEMLKDLMSHKYLSQAPPKSTGREVFGQVYLDNMLNKYKHVDKYDLIAT
                     VTMFTAKAIYYNYKNFILPKVNVDTLLIGGGGAHNLTLIGYIKELLLEVEVLTQDEYG
                     YSSDAKEALAFVILGNETLNNSFSNVISATGAKNKVILGNITPKPFGGK"
     gene            complement(17612..18631)
                     /locus_tag="ACGI59_001639"
     CDS             complement(17612..18631)
                     /locus_tag="ACGI59_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HFG9739428.1"
                     /translation="MLVKYLKGRSKYIALPLVVVSIINIYLFAIDIFKNKYSELIYLD
                     FLVLFIIMVFFVIDYINFRNSYTNLYNCIENSGEIDSYLVDGQSFEENLIKDIIENLK
                     IKNNSDIETYKQSLKELDEYIAKWVHEIKIPISSLSIITDRLSSIEDSLDIKNQVAKI
                     NFLVNSILYSSRSNTMFEDVFINKFNLEKLIKMSIKNNSFLLIKNNVEVSLNELENDV
                     YTDSKCMSYVLDQIINNAIKYSKEVGKIEFNSKKLENGVVLSIKDFGIGINEEDISRV
                     FDKGFTGKNGRNQLYKSTGMGMYFVKKMIDSLGHEIEVCSENGSYTIFNIYFYDISDY
                     LSLDS"
     gene            complement(18625..19305)
                     /locus_tag="ACGI59_001640"
     CDS             complement(18625..19305)
                     /locus_tag="ACGI59_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFG9739429.1"
                     /translation="MYRILLVEDDIDLSKEIALALEKWGFKVELIDDFEVVLDEFIDK
                     KPDVVLLDVNLPLYNGFYWCEKIRAISNVPLIFLSSRDSDMDLIMGINNGADDYITKP
                     FSIEILVTKINGIIRRVYNYSDSNSILYCEDLMFDVGKGIIKHKYKDKSIELTKNEIK
                     ILTLLLKNKNRVVSRESLMMTLWDNDEFVTDNALTVNMNRLRSKVKELGFDDFIKTKK
                     GVGYIIQC"
     gene            complement(19457..21364)
                     /locus_tag="ACGI59_001641"
     CDS             complement(19457..21364)
                     /locus_tag="ACGI59_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFG9739430.1"
                     /translation="MSILGIAYNNFKSNIRTYLAFFISMVFSVVVLTNFELLRYGDAI
                     NVLQDENKKFTLSVLVAVIMILSVFLFFFIWYATNIFFKNRSKEIGILSFMGLDLYTI
                     GKIYFVENMLIGISSCITGIIIGIITSRFFQVVIIKLSGFNIKVSNGLSIEAILYATL
                     IFISIFIIMAVKGFITICRSSVINLINISKKQEKIPKVGIGIYILAIVSIIVIGYGYY
                     LSTNIRTGDITSVLPVIGIIIVGTYGLFKSVMPVIFDIIIKNKKILFNGNNIIAINNI
                     NYRLNKNYKTYAIIAIIITTTISTLGASVAMKRIHKNAQEQRNVYTISIVSMDKDEVN
                     KKQIENIIKDTNKIKYSVNPELTILKKTDIKEAYDTTDTIMKYSDFCNTLKVNGNDKE
                     LKEYGERLVSGNNVIELKSPQTIASIATKEAKIKLDGQLYNISRGEVKVPVLGTGLNQ
                     SIIVVNDDTYFKLRDKGQQVYFYGAKVFQEENSQVMFNNIEKNLSLKNGYIQNGYKAQ
                     GESQWMKFAYVVLVFLFLVFAIVAGSIIYMKIYSDAYEDKEKYKILLKIGTTEKDINR
                     AILKEVAMFYTLPMLSATISSYFALRLAGDLLMTDLFGIYILSLVICLIIFIIYGAVS
                     VNKFKSVVYKN"
     gene            complement(21365..22117)
                     /locus_tag="ACGI59_001642"
     CDS             complement(21365..22117)
                     /locus_tag="ACGI59_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9739431.1"
                     /translation="MKEILKIKNISKDYGIKGFKTNVLKNISLTVNEGDFISIMGPSG
                     AGKTTLLNLMSTLDKQTSGEIILDGINISKVKNNELSKLRREKIGFIFQDYNLLDNMK
                     LMDNIALPLALGKKKSKEIEAKVFSIAKKFGLENHLDKYPYQLSGGQKQRGAAARSLI
                     TNPTVIFADEPTGALDSKSAYELLESLEKINRENNATIIMITHDPLTASYSNEVYMIN
                     DGNIKCKLNKGNSRKEFYGKIMDMLASMGGEM"
     gene            complement(22433..23587)
                     /locus_tag="ACGI59_001643"
     CDS             complement(22433..23587)
                     /locus_tag="ACGI59_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFG9739432.1"
                     /translation="MINKAIKFKFIIFYFFSYIIFAFASTKFTPFLSKLGYSAFERGI
                     ILSSYAITNILFQLLFGVLADKYQTMKKIVLISLSVYGIASVIMFSTESATFIIYLLL
                     VALSGGLLNTCCSLYDTWIIGCGDNINNYLSFIKTFGSIGWAIGSMVASYLILRFSYF
                     GLSVAIGLIVVLLIINILLLPDIEKINKKINVTNKDIIELLKDKKYILLVCILFLLYS
                     MVVANNCTVIDKMIALNATNSQISLKWSLQSLLEIPTYLAGSYLLKKYSSLNLLKLSA
                     IMITVQFLLFALTNDSNIIIILCVFQVFSTPLILITSKRLIFEVSPKKLRSSSQLIAL
                     SIFMGGSSLIIPTVAGFLSINIGYNYTLMVLSLFGCLAYCLIVLLNYLQE"
     gene            complement(23590..24057)
                     /locus_tag="ACGI59_001644"
     CDS             complement(23590..24057)
                     /locus_tag="ACGI59_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFG9739433.1"
                     /translation="MFFKRKKEAITINSYIDGKIINLGEIPDKVFSTKVMGDGFAMIA
                     KDNILKSPTKGVISFIAQTNHAIGIKLQNDVQILIHIGLDSATTKKENFKLEVKQGQE
                     VNMGEKLVEIDNKLLENTLYIPLVIVENPNTVKYHLSNLNQEGNSGIKVINFD"
     gene            complement(24071..24559)
                     /locus_tag="ACGI59_001645"
     CDS             complement(24071..24559)
                     /locus_tag="ACGI59_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9739434.1"
                     /translation="MITNKIKNITAVLKDENIFLSVVMVCFLILPVLYIVYLELTSLF
                     TNISFSQLLNNDVNASLNLISNFSAVYSAYVIYNIKKNKEIKENMIAFYLVLLGQIFQ
                     IYTITIVIMFTYIYKFVGIKQIISSIKELQWGKSYKALFSSIAVCLISLFVMIIRIKL
                     IM"
     gene            complement(24573..25904)
                     /locus_tag="ACGI59_001646"
     CDS             complement(24573..25904)
                     /locus_tag="ACGI59_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HFG9739435.1"
                     /translation="MERKRQRILIAGGGSTYTAGIVTMLIESVAKFPIESIKLYDNND
                     ERQRKVAEACAIIVREKNPEIKFSYTTNPEEAFTDIDFVMAQIRVGLYALREQDEKIP
                     LKYGVVGQETCGAGGIAYGLRTIGPIIEMIDYMEKYSPNAWMLNYSNPAAIVAEACRV
                     LRPNSRIINICDMPVCLEEIFARVLGLNSRKDFDVRYYGLNHFGWWTSIKDKEGNDLM
                     PKLQEYCAKKGYEEFTPQGQHKESSWLETMRAAKDLLEIEPTTLPNTYLKYYLMADET
                     VEHANPNYTRANEIMDRREKDTFEECERIIKNGTARDTWFDASEHSQFIVELACALAF
                     NTQERFLLIVPNNGAIENFADDAMVEIPCLVGKDMVEPMSIGKIPTFQKGLMEQQVAS
                     EKLAVEAWIEKSYQKLWQSFTMSKTVPSAKVAKEILDEMIVANKDFWPTFK"
     gene            complement(25918..27483)
                     /locus_tag="ACGI59_001647"
     CDS             complement(25918..27483)
                     /locus_tag="ACGI59_001647"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucoside-specific PTS transporter subunit
                     IIBC"
                     /protein_id="HFG9739436.1"
                     /translation="MMKKIQRFGGAMFTPTLLFAFAGIMVGFSIVFQNQSIMGSLATP
                     ENIWYQFWGVISSGAWMVFNQLPLLFAISLPIALAKKQQARACMEALATYLTFNYFVG
                     SMLSFWGKSFGVDFAAEISAGSGLVSIAGIKTLDTGMVGALLISGIVIYIHNRFYDKE
                     LPDFIGLFRGSSLVVAICFFVMIPVALLTCFIWPHIQNVIRHLQTFFINSGNIGVWCY
                     AFLQKILIPTGLHHFVYAPICYDSVVVPGGTSVYWATHIQDFQTSAKTLKEMYPIGFS
                     LSGLSKVFGSLGVFGAFYVTAKPEKKKKVLGLMIPATLTAVLTGITEPLEFTFLFVAP
                     LLFLVHAFLDACLQTISFALGVVGDFGGGIINWVVLNWLPLGMYHWKVYVVQVVVGII
                     FSFIWFFVFTFLIKKFDMKTPGREEDSEETKLYTKNEYLETKDEKGNKLSKASQQASE
                     YIKLVGGAENVVDVTNCATRLRLTLKDDSIISKEEDFKAVGAHGLVHNGKAIQIIIGL
                     SVPSVREEFENLL"
     gene            complement(27653..28438)
                     /locus_tag="ACGI59_001648"
     CDS             complement(27653..28438)
                     /locus_tag="ACGI59_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFG9739437.1"
                     /translation="MRLEEMIFLNYNKLNDTDLLIWKYITANKKHCSKMSINELAMKC
                     NVSRATITRFVKKLDLKGFSEFKVLLSWESEKVHVIENQSFDIACNSIIKYVEDQKNK
                     DYDALCKLLYESRTIYAYGSGDIQNVVAKQIKRMFLSCQEIIYDFEGRTFDDSFYNIV
                     SKNDVMIIISLSGENENTLNIARRLKLIGTKIISITEFKNNTLTDLSDESIYISATNI
                     SFLQMHPSYKLTMLYFILIELLFIKYSIYKRHRMITEGIDCKL"
     gene            complement(28605..29804)
                     /locus_tag="ACGI59_001649"
     CDS             complement(28605..29804)
                     /locus_tag="ACGI59_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HFG9739438.1"
                     /translation="MKKKVLFLVIILAFIILLGNSTYSQKNQLSEEEKVDDFNYVYNV
                     VKTGYPYLGVNKRLNNIDWLANKDEYTKRIKNTENDEEFIEELSSILSDLNNKHTEVI
                     DNKKRYELFKKSYSNNDWYDFLNDKKVVDRYDSMNLKIKMPDDIFVKKELILKDVIKG
                     QIGYMYLPSMSSKNGSINNDLKIIGDYINTLEKHKALIIDIRGNLGGSDRYWQGIVSK
                     LIKNDVKINGYRIYRNNNEIVKKYTNARNIKLNPVENLPASVKENAPKEIIKDFNDFE
                     DTSYTIKSTDDSKFKGNIYLLVDRKIYSSSESFAMFCKENKFATLVGETTGGDGGCID
                     PVLFNLKNSGLIVRMASCMYLNKNGICDEEFKTIPEFKVKDCKRTADFKDDNCIRKAL
                     ELENINY"
BASE COUNT         9948 a         5183 c         3006 g        11841 t
ORIGIN      
        1 ttttctaaat ttaggtatgc tttactatat aaaaatgatt ttttatctta atcttctgaa
       61 tataagtgaa tgctatcatt ttaagtatct tataagcaat gtaactatat atctctaata
      121 actacttttt taacatgctt aaactattta taagctatta tatttatgta ttaacctaat
      181 aactttttca ctaaaaaata acaatctatt caaagataaa ctatagtact tcttctactt
      241 tgtttcaata aaatactaaa taaatttcgt acatccacaa tttttcaggc tacactataa
      301 atatacaata tttatcttag ttataagttt aagaatattt ttagtcttat tttgtcgaat
      361 atctttttaa taaaacttca taattctaag catattttta cactgtgttt tcatgtacaa
      421 aaataatgct tacaaataaa caacataaat aaaacttaaa taagatggat tctattcaac
      481 atagtatcca tcttattcat tcaaaaccta aaaggatttt ttataatatt atatatttta
      541 aaatctaagt aactcattat agtatcaatt tcacttaaag cttacaatac acttattaat
      601 cttgttttat attaaaaaca tctacaataa caatttaaat ctttaagtga tatccatctc
      661 acacctaaag gagtaggctc tccgcactat tctgtaattc ttcaatacaa tcattattat
      721 tttttatata atttaattat atattttatt ttaccaaaac cagcatacat aaataatact
      781 taaaatagat taaataaagg atactaaaac actttattga ggtatatgac ttgaaatata
      841 tgaactaact tcattaggag aaatatctaa aatagttgca aattcttcat ttaataacct
      901 ttcagcttct ttcattatat tatcatctgc ttgatgtgtt tttttaccaa tagattttac
      961 atactctctg ttattatcta tacttcttac taattttatt aactcttcac atttaccacc
     1021 tctgagtatt tttttaaatt gctcacttct caatttttca ttatctatcc atgatgtatc
     1081 catattagga atatcattta ttaatgaaag cacattaatt ttagaaagta actttctcat
     1141 tggtactttt tcattatcta caggaatttt tattactgta ttattagaat aaataggact
     1201 caatacataa tactcttttt ttattccatt tgtaaatcgt tcatttgtta tatccatgac
     1261 tttgcagact cctgtcatac catacattat ataatcatca atcttaaaca aaaacatcac
     1321 catccttaat aaaaaaaata gttttgacaa ctaaatccac cttgccaaaa ctatctgcta
     1381 tatattatat tttaacacat tttgagactt aaatgtaagt aaaacttata ttattaaaat
     1441 taaaaaatac attaaccatt gattaatgta ttttttagat ttttataaca agcatatcaa
     1501 aataaattaa tacagcttat ctcctgtgaa tacctataaa attggtgctt ggctattcat
     1561 tgagtaccca tcatttctta catcatttat ttctaatatt tcatcatttg ttttaacctt
     1621 agtaagtttt ttaactgctc cataaggttc ttttaatgaa attttatctc ctcttccaga
     1681 aataacccat aaaattttgt accctctagg tattacttgt agtttatttt cacctttacc
     1741 atcagtaaag tagactaaca aattaatctt tttattatta gcatactcaa agactggaga
     1801 aaatctagta cctccaccta tcctaattct atcttttata tctttttcag ttctcacttt
     1861 atatacacgt ctaatttcat tatcacattc tataattgta atttcatgat tatagttttt
     1921 cactatacta agaacttctt taatagcttg tttaaactct tcatcactaa tacttccact
     1981 tacatcaagt gcaactgcta tttctgactt atgacttcta agttctcccc tcaaatctaa
     2041 acgatttggt tgtcttctat ttcttcttgt tatagtcttc tttttattat tttcaactgt
     2101 tcccattatt ctattaagat ataaattcca tggcaattca cccttactat ttttaagtga
     2161 tgatatcata ctttctaaat aagcaggaac tttacctttt tgagaactgt taataaattt
     2221 ttcagtaaat tctctaaggg tattttcatc tatctcattg gattcatccc atatatcatg
     2281 agttctttcg gggctataat caatttctat atctccatat ttatcactat catcttcctc
     2341 accatcttca tccacttcca tcaaatctaa ttcagtctga atcttttcta cataatattc
     2401 aaaaggttca tatggttcaa gttttaagga atattttaaa tttacccact ctaaggtagt
     2461 tgcatatggt ggtaaattgt ttagaaattt atttactact atatccattg ccatattaat
     2521 tgctaatgta ctatactttc cttttaactc ctttgctctc atcaaatgca tagataatat
     2581 atgatgaatt tcatgtttaa ttgtagtctc catttgcttt atgtcaagcg ttaaaaagat
     2641 tattggatta aaatatataa tatatttagc tcccttaaaa ttcacacctg taggactact
     2701 tatatcaaat cttatttctc ttgacatctg aaacaaaaaa tatccataaa aattatcttc
     2761 ttcttccata agacttaaat tcactttatc tacaagacta aaaaactcat acttaaaatc
     2821 ttgtggtata tctatatcaa atttctcatt agcacgattt gcctttagca tagaatgagt
     2881 atttataatt tcacttgctt tattataaag ctcttttgct tgtttatcaa aataattttc
     2941 catacattat ccccttatta aactataaga ttcaaaatat gattttacaa actcttcatt
     3001 ttctatagct tttttatata cttcaatata actgtttcta atatccttca taatacctat
     3061 cattaaatct acaggataca tttttaaaaa ttcaataaat ctattaatat agtggtttga
     3121 ttcatattta tcattcttaa tatttaattc taagtctttt agaatgttca tagctgatag
     3181 ataaagtctt gtatgactct cattttttac tctttctaca atagactcat caatagattc
     3241 tcctaaaaaa acatcttcat aggatattaa tggttcagag tttgattcaa taaaacttat
     3301 aaattcttct gcaataactt tacctacatt accctttaca acatttaaaa atactgctct
     3361 aggaatagaa ttattttttt ctttataaac tttataaatt ttagatactc tctcataact
     3421 tctaggagtg gctcttacat catcttcatt tattttatgc agatattctg gaaaagtgga
     3481 aataaactcg ataacttttc tttcaatacc ctcatctatt gcccatttta tccattgtgt
     3541 atggtcactc tccatattta accaaacaaa cctattttct tgggctgcat ccatatctac
     3601 cacttgataa tcaaaatctg aaccatattt acttgatgga ttcattgctg ctaaaatctt
     3661 tacatcatca tgcagtttat atccattaat ttctctattt aaaattaaat tcataagttc
     3721 ttgctgtact gtatgctcac aacgatttat ctcatctata aataaaagaa ctgtcttact
     3781 tttagatatc tcttcatcaa tttctcttaa tttgttatga acagcataca ctgttgtttt
     3841 cttttcagtc ttatatccat tagaattaac tcctacataa gattctatag ttggaagacc
     3901 acctatttca ccctctttaa gtaagtttcc atcaataaca attaaactcc aatcattttc
     3961 tttggcaagc ttatttgcta aagctgtttt tcctattcca ctttcaccta ctattaaagg
     4021 tacttctcca gtggataata ttaagtcaac gctttttaat gtgtctataa aattcatttt
     4081 tctctctcct acataatctt taatttctca tctacagcta ttttttttgc tgtcttactt
     4141 ccatatttat aaataaacat aattttatct attggtatac tttcgctatt tctatctata
     4201 acactacaat tcaaaaaatc tctaatatac ttttcctcaa catcttcttt ggataatact
     4261 tcttttaatg cactctctaa ttcatcttta gatacttttt tcagaatgta cttaatcact
     4321 aaaatattca cttttaaaaa ttccaacatt ttatttttat ctaaatcagt tataaagcta
     4381 atagctgctt cattattttt tatagctata agttctgcat tataactagg tgaatctata
     4441 tatctcaagg catcataact tattctcact gcctcctctt gtacactttc ataaggttgt
     4501 tttataaact ttatcgaatc ataatttttt cttactgcta acagttgtaa ttcttcactt
     4561 ggatttttag catatttaat tgcccatcct gcttgattta tagccatttc aataacttta
     4621 tcagttgggt ttttgatata atccagtata ctccatccct catttactgc ttttatcatc
     4681 atatcttctg ttggattatc tataaattta attgctctaa tattattatc tattgctagt
     4741 tcttgcatct ctctggttgg attatttata tattctagta caagcccatt ttctttaata
     4801 gctaacaatt tcatttcatc agttggatta tctattgtag ctataatatt gggattattt
     4861 tttatcattt caataactct caatttttcc ataatgtgcc tttccttcta taattatttt
     4921 tatatctata ttaattataa taatctattc ctatttatat aattgtttct tattataatt
     4981 aatatacttt ttaatcaaat ttaaacagaa taattatacc aatatataaa aaaatttcta
     5041 aaaaacaata aaattaagtt ataaacacca atatatttct attatttcaa taagtataca
     5101 attagattag aatctaacgc tagtaataaa aaattacttt cccaaaatag atataagcct
     5161 cattttatcg ttcaaataat gaaaatatat ttgatttcat gcaaaaagag tgcctaatga
     5221 actaaaaagt tcattttgag acactcattt ttttatataa atttatatag tttttaatcg
     5281 ttcgcattca accactcagt taagactttt atatatttct cattttcttc aacgaatggc
     5341 atatgtcttg aatactcaaa taattcccac ttactattag gtattctatc atacatagtc
     5401 tttgcaatat atggtgaaca taaatctatt gctccactag ttactagaca aggctctttt
     5461 atttcatgta atctatctgt aaattcatat ccagaaagag ttcctgtagg agaaaattca
     5521 ttttgtcccc atcctacaat atatgcttca gaacctgatt ttttaggtct tctcaggcac
     5581 tctggagaat cttctgtcac ttcacctgca caatacattt ccataaatct ttctaatgca
     5641 tcattatatt ctttgctaga gtaatctcca gtatttactg catcaagtaa tgctttttgg
     5701 tcaacctcac tcatatatga tattcttctt ttttgttctt tctcccaaag ttttgcagaa
     5761 gaaagagtag atgaaaggat gtaagactta ataccttttg gtttatattc aatagcatac
     5821 caaatagcct gcatacctcc ccaagattga cctaacagat gaatttcatc aagtcctaga
     5881 tgttttctta gttcaataag ctcctctatc caagtatctg cattaaatag ctcaggatgc
     5941 ccttctacaa atgaatttcc acaaccaatt tggtcataca ttattacttg tctaccactt
     6001 tcagctatct tatccaatac ctcaaaatag ttgtgtgtag aacctggacc tccatgaagt
     6061 aatactaatg gcttcttccc ttccgtattt tctcctacta tacggtaata cgttttaaaa
     6121 cctttaaaag gcatatatcc ttcagtaatt ttcatgttta tctcctccaa tttgtaatta
     6181 taaacaataa tcaagttcat taacaatact atttaaaacc tcttatacat atttgattat
     6241 gtattttaat ctttatacct acttttataa atagtataac ataaaagtta aaaatctcta
     6301 tactaaacca attataaaat tatattaact atataatttt attttttaat aatagtatat
     6361 acatatttga ttattttcat ttgctattta aaatcaattt taagttttct tcttataata
     6421 aacttcctct ttgtggtctc atgttagtct ctaaatttaa aattttacaa aaaattttca
     6481 taaaataaaa ttatattgat tgtatctaaa caaatagggc aataaaataa ttaagtatag
     6541 ttacttacct ttcaagcaat attagtttta tgttagaatt ttattatatt gatttacagg
     6601 aggtttgcat ttgaataata ttaattcact taaagtaata aaattatctc ttggatgttc
     6661 tcttgctata tttattgctt ggctattaaa attagaatat tcaatggttg ctggagttat
     6721 agtactactt acagtaaagg acactaaaag agaaactctt aaaggctcca ttggaaagat
     6781 ttatggtttt cttttatgta ctattttttc atatttatgt tttaatattt taggatataa
     6841 tttgacttca ttttcaatat ttatatttat aattatacca ctttgttttt tgttaaatat
     6901 tcaagatgtt attgctatgt gtgtagtcat agcatcccat tactaccttc aaggagaaac
     6961 ttctatcaaa tggattttaa atgaagctgg aatatttgca gtaggaactg gaataggtgt
     7021 cttaataaat atgtatattc caacaaatat acataaaatc catgaagttc aaaaaaagtt
     7081 acaggaagaa gtgagtattg tacttataga cattgcagat attattgtaa atcctaaaaa
     7141 agaaaatgga tatagtagag atttatatac attaaattca cttattgaca gcagtatttc
     7201 tgaaacttac gataatatta ataatacttt attaagtgat actagattct tcttagagca
     7261 tatggatatt ataaaaagtc aacgggatat cttagaaaat ttgtatagtt atgtatctca
     7321 actaaattct acaccaccac aagctcatat tctatctgct tttatacata agataggtta
     7381 tactgaattt gaagctgaaa caggcaatct tcttcttgaa gaattaaaga gactaatgct
     7441 tagtatgaaa aaccagcctc tacctgttga cagaacagag tttgaaaata gagctatttt
     7501 attcctatgc ttgactgagt taaaacagtt tttagttaat cgtaaacatg ctcagatgct
     7561 tagagataat aatttttata aataaaaagg ggagaaatct agcagttgac ctgactacaa
     7621 aatatcagat ataatattaa atgcgcccct agtaagaggg cgcaatgttt ataacttatt
     7681 ctactgtcat gattaaatca ttattcatat cgaacataca ttatttgacc aagtccaata
     7741 ttagcttttt catcagtgat ttctatttta atagtcacat catttttttt acagtacttc
     7801 gctaattctg caccagaacg atttttacca ctaaaatcta aagaaaatgg cttatcatat
     7861 ttaaaaggat attcaacagg tttaccctct ggtgctaatg caaacatccc atatggtgtg
     7921 ttcataaatc ctattacagc aatcgtaaga tttgatgggt ctaaatttga ttcactacct
     7981 gtaaatgtaa ttgtatttac tgatgaatta actacactac atcgaacacc ttcatacatt
     8041 acatgttttt catttatatc ttctcctttt ttcccaaaat ttccaacagc ctttgcccct
     8101 tcttgatgtg gtaacatagt gtgcttccca ggtggtgctg gtttctgtgt ctgttctggt
     8161 tttaccgtct gtactattgc accaactttt ggatatgctg tcttatcaaa tggtaatggg
     8221 tggctaagac tcttgtatat gatatccttt cctgctgatt tattagcatc aatatctaca
     8281 tgatacaaac gcccatcacc aaaacggata agattccact ggtggtcatt tgatgtgctg
     8341 tgtacataat aagattcaat tcccatagca tcacacaaag ctttcatgct cctagccatt
     8401 cctgagcatg cacctttttt agtaaccaat gtataggcag tatttgctcc ctctactgca
     8461 ttccagtcaa catatgttgt gttattttta atgtgattgt aggcaactaa aatcttagca
     8521 tctgtggtca tattctttgt gattttcttg ttcataaaat ctgctgttgc ttgtgctact
     8581 gagtttagtt gggcttgtga cagctttgga ccatatacgc tattggttcc agtgacatag
     8641 ctttttggtg tttttgctgt tacctctttg gctgtcatac cataaactgt catactcata
     8701 cctgagatta aaactattgt tgtaatcaat aacttggtag ctaacttcct aattttcttc
     8761 atacttaata ttctccttta taaaacattg ttacttccta ttctctacca ttaataaaat
     8821 taatttagga tttaaaaagg tggattgtca acatgtatat ttctccattc atacattcgt
     8881 gaaatattta aatttcatgg aaaatctaat agatattttc tagataattt gtatacaata
     8941 caactcgcaa atcagggtgt ttgctgttag tttgagaaaa tgatgatgat taatacattc
     9001 ggaatctttg acctataata aataccattg tcttcaatca ttaataatgg taagagtaaa
     9061 gagaattatc aataatacag ttaaaatcaa ctttgtatac atttcataca ttctctccca
     9121 acaacattat acttcttata atttgtatat tcaatacgtt ttctgggaaa atataggata
     9181 tatctataaa aatataaaaa aggagttgaa cttaaaattt gattaaaaaa aatcaatttt
     9241 aagacaactc atttgtatta taaaaatgct atatattatt attaagtaag tcaatatttt
     9301 agatatttat ctatttctta ttgacttacg cttatagtct aacttatttg tttagttgat
     9361 atataaccta gttttaagca ttattaatat ttaaataatc aagtatcctt tttgtttctt
     9421 gtagtttctg ttctgcctca tttaagtttt cttgaattat agaaatatat attatatcta
     9481 tcaacgtaag cattgctatt cttgaagaaa tagctccttc tctaagcctt ttttctatat
     9541 ttggtactac tagttttaaa tcactaatct tactaagatg attgtcacta tatttagtta
     9601 tacaaatgac cttacatcct tttaattttg cattttctat agactttatt acttcttttg
     9661 attctccaga gtaagatatt gctatagcta tatcttcttt atctacattt gaagatacca
     9721 taagttgtgt atgactatct aaagaggtaa atgcctgttt atttatcctt aaaagtttca
     9781 tctgtaaatc tagtgcaact aaagccgaac cacctactcc aaatatatat acatttttag
     9841 cctttattat ctgttcaact gcttctttta ttatatcttt ttcattcaac aaccttgttt
     9901 ggttgatagt ctctatgttt ttgtttgtaa ttttgtttat aacactatct atattacaat
     9961 catagttttc atctacatat gtgtattcat tttctttgcc catcaaatct ttagctatct
    10021 caatttttaa atccccaaaa cccgaatatc ccatttttct agaaaaccta actatacttg
    10081 ctgtacttgt ttttgagttt gacgccaatt ctgatacaga aagcttactt atagcctctg
    10141 tattattaag tatgtaatca gctatttttt tatctacttc cgttaaatct tcatatatct
    10201 gttttaattt taaaatgcaa ctcatactct atcacctcta cctatttact ataatatttc
    10261 ttcttagcaa gtaaacatgc tcctaaatct ggagtcaaca ctggtctcac tataccaaat
    10321 ctcgttttat tcaaatattt tttaagtggc tctaatatta aatctcctga cttaaacact
    10381 ccacctgaat aagatacctt tatctttgta ccttgttcaa aatgatgctc taatcctatt
    10441 ataagcctac ttaattcata agctgcatca tcaaatattt tactagcagt attatcacct
    10501 tcaataactg cttttaaaca tatcgtagca aactttgcta tttctattct atctcctttt
    10561 atttcatcat ttacatattt tattatgtca taatcattct ctatattaag atgtcttttt
    10621 attaaatcat ataattttgt ttttgaacac cttccatcag actgcattgt gaacattttt
    10681 aaggtagcca ttcctatata atacgctgaa ccttcatctc ctaatacata gccccatcct
    10741 ccacatctgt gaagattccc atctttatct aaagcctgtg ctattgaacc agtacctgca
    10801 attatattta tacctttttc cccatttaaa gaacctgcta atgctatttc tacatcatta
    10861 cctagagtat aatttatatg actatatgca ttttttgttg ctacttctaa tgcatacaat
    10921 acttctttta tttttccata tccagcaagt ccaacaaatg catacgttat ttcttctaca
    10981 tttatcttag catctttaca aacttcttcc aacccagtta ttaataattt cgttagatta
    11041 tcaaatccta tttgattata atgacaagtt cctttggtta tggttcctac tatatttaat
    11101 tcttcatcta ctaatgtaaa tgttgttttc gttcctccac catctacacc taaaaaatac
    11161 atactatcct cctacaatat ctttaataat tctttcaata cttaatatct aaaaataacc
    11221 ttccaatgct gtttttttaa tattgaaagg ttatcagtac tattatttat tttacaattt
    11281 ttctattgat tttaaagctt cacttatata accttcttgt ctatctagga tactctttgc
    11341 actatcctta tctaaaccac tttttatcat aaatatagcc aatttaacgc tattatctgc
    11401 ttcttctaag aatttttctg caacatcata ttttacattt gtagctatca ttattattct
    11461 ttttgctctt tcaaccaact tttcatttga tgtcttaaca tctaccatca agtttgagta
    11521 tacttttccc atcttaacca tagaagctgt tgttaacata ttacatacca ttttttgagc
    11581 tgtaccactt ttcattctag ttgaacccat tattacttct gcccctacta taatgtttat
    11641 tggtaaatcc gcttttttac tcatttcact attcggattc atagttattg atagtactgc
    11701 acaccccaat tccttagcat actccattgc acctataaca taaggagtcc ttcctgatgc
    11761 agctatacca catataacat ccttagatgt aactcctcta tctattatat cttgctttcc
    11821 aagttcttta tcatcttcag caccttctat tgctttaaat attgcttgat ttccaccagc
    11881 tattatagct tgaacaagct caaaatccac tccgtaagtt ggaggacatt ctgtggcatc
    11941 tagtattcct atcctcccac tggtacctgc tcctatatag aataatctac ctccctgttt
    12001 cattctatct actattatat taacagcttt tgctatactc tccttttgtt cttcaacagc
    12061 ataggctaca gttttatctt cgttatttat taattctatt atgcctaatg tatcttcttt
    12121 atctatttgc aatgtattct tatttctacc ttctgtaact aaattttcta aagtttttat
    12181 taacatatca tttcctcatt ttcttaatat taaattttta aacacatttt aattccaact
    12241 ttcataatta tatttttata ataaattaat attttcataa taaattattt tgttgagggt
    12301 ttaaaattaa acttttgcca tggtttaata taatctaata taaataattc ttcttttctt
    12361 atatatccta ctacattcga tttaccactg tttttcatat cagtcaaggc tatttgtaac
    12421 tcccctgcat aatgaccata ttcactactt tcaattacta tatctcctct ttttattatt
    12481 ttaggagtat taaatagttc aaatttatat cctttatatt taacacgaga ttgagtagac
    12541 cttatcagac tatcagatat gtcccctcta ttaaaatgta tttcttcaaa gagtatcttc
    12601 ttctcaactt ctcgtatatt ttcctctatt gctacatcaa atgtaaccat atcttttctc
    12661 atagaaccta atacctttag ctcctcatat gttggataac aatttgatat tattatatca
    12721 tttatattat tcatagctat tatatgcttt acttgaatat ctataggtag atttctgtgc
    12781 atctcaattg ttggaagccc atcagttaca ggccatggtc caaatgtagt ttcattttga
    12841 cttgttacaa aaactgctgt tcttaatcca tacttattaa atctttctgt acattttata
    12901 aagtgttcta aatttaatcc actatatcta tgtggataaa agttatgaca tcctatcaaa
    12961 ttttctttat ttggttggta gtccattatt gtatctatat aattagtatt attactcata
    13021 ttgatttcta ttttaagatt ttgaggatta aaagtcatta atgcctcttc agaacctgta
    13081 aatcccatat caagccttat tccgtctgcc ttcgtctttt taaaaaaatt caaatctttg
    13141 tagcttattt ctaattcacc aaatactctg ggatttacat caagtattac ctcaaaacct
    13201 ttaaacttag cgtattcatt tatactttta aattttttaa ttatattttc tttagtatct
    13261 ttaactgata ataaacatga aaatatacgt gtaaatccat tttcagcagc tttatctata
    13321 taatcatata tttccttaga tgttgttttt tctggataaa ttgatattcc taatcttccc
    13381 atctgtgtca tctccttata tcctatatgt gtttaaattt atttcgaatt acacgaaact
    13441 tttttaaaaa tttgatgaaa aaaagtttca tatatattta taaaaaaaga aaagtttacc
    13501 ttttcttttt ttataaacaa tcaacaaaat atcttcgtta cttatttcat aactgttata
    13561 ctttgaaatt tatactaatc taatatagtt tttaatttcc aatatagttt ctaattaata
    13621 agcttcttgc tcttcaactt tttcttcatg aagcaacctc ttatcgtaca tcttgacaaa
    13681 tggatagtaa attattattg atatagctat acataataag ttaagaactg aagctctcca
    13741 atctccacca gtggctaaat atgcccctac tggtcctggt aatgtccaag gagcagtaac
    13801 tactactctg gatactaaat tagcactcat agcaaaccat gaaactgttg ccattactat
    13861 tggtgctaca acaaatggta ttattaatat tgggtttaat actattggcg ctccaaatat
    13921 tattggttca tttatattga atattgctgg tattacagtt gcttttccta aagactttcc
    13981 ataagctgat tttgctctaa atgcaagaag taaagctaag cctattgtag ctccagaacc
    14041 tccaatccat ataaaccatt gaaaaaaggg ttcagcagca atacctggaa ctgcttgacc
    14101 cgcagcatat gaagctgtat tagcatctaa taattgtatc cataaaggtc ttgccaatga
    14161 acctattatt gatacaccat gtattccaaa tgaccaaaaa aacgttatta aaaatattag
    14221 tataattact gatggtaatg tgtcagtggc tgaaactaat ggagttacta atttagctat
    14281 gaagccatgc caatcaaacc ctaggaaata agtaacaata gccattaaaa ttattattaa
    14341 tgcggctggt gtcagagctt caaaagacct tgtgactgat gctggaactg cttctggcat
    14401 ggttatcttg aatttagatt tgtcagtaaa tctataaatc tcaacaccta ttatagatgt
    14461 tacaatagca acaaacattg aacctccacc aagattagcc attggcattt gccatccttg
    14521 aggaattgaa ttataccaag ctaaaagttc tggactcttt tcaactatct ttaataaatc
    14581 aggggtaaga ccctctattg cagtaggaac tattgtcaaa aggaatgtag ctgtagctaa
    14641 taaacctcct gtcaatccat ctaacttata tgactttgcc aaactataac ctatgccaaa
    14701 tgtagaatat actgatataa taaaaaatgt cattctatat ggtagtaata ttgtaactgc
    14761 attttccttt agatactgag ttacccccca tgaatctggt aatggaggaa atgctaatat
    14821 caagaaaaat gacccaacta ttataagtgg taatgaagct acgattccat ctcttacagc
    14881 tcttaagtga cgttgttctg ccaatctggc catagaagtt gataatttag tgtctaaaaa
    14941 ttctgtaaat ttatctagca tcttttctct ccttaattaa tatttatttt tattatcatt
    15001 aaaattttta taaaattaac tttcttctaa tctaacaaca ctgcaactac tatttactca
    15061 acagctcttg aacttgtttt aataactttg gtcctcctaa tggactataa gcttgtgctt
    15121 gtattaatgc acatggtaca ctaacctcat cagcaaaagc ttttaaatag tcaaatctat
    15181 gtctaacttg tggagctacc atagcaatat cccatccatt tttaacttca tcctcaaact
    15241 cttgagtacc aacagccaaa acatctatat ctattccatt ttttgctcct tctttttgta
    15301 atgcattaac tgctatagca cttgacatcc ctgatgaaca aacaaataat actctcatat
    15361 tgattccccc tgttttttta tttcttttca atcattatag catgtatttt tgattacagg
    15421 aatacgtttg tatatatttt tttcatattt tacattttaa attgtaattt tatttcatac
    15481 atttatatct tgctttaatt gctttattta tctaatcatt cttttgtcta aaattacaca
    15541 ctacttaaaa tactcatatc ttttcttaaa aaagaaaaaa gactatctta ttattaacat
    15601 aaaatagtct caatactttt ttaaatatgt attcctttat atatccagaa gtaacctatt
    15661 attgcaaatg ataaagcaaa taaaatatac agtctaaaaa atcccaaaga aaaacctctc
    15721 ttagtttcaa caaagtatct tgtatatgca actgcaaaag atgtaattaa tgaatttcct
    15781 ataacaactc caaatctatt atctattcca aatatagata ataacaaagg aaaaactaca
    15841 gtacttacta gagatgaaac tattaatatt aatccacttt taaaacttat agttggaata
    15901 ttataatctg ttctttgatt tttctccatt tgtttcttag tatttttatt actttttgta
    15961 ctttttgcat ttttataatt attatttttc tttcttttct tttttttatt attagacatg
    16021 tcatttacct ccaaaaggtt taggtgttat attccctaaa ataactttat ttttagcccc
    16081 tgttgcagat attacattag aaaaagaatt atttaatgtt tcattgccca agattacaaa
    16141 tgccaaagcc tcctttgcat cagaagaata cccatattca tcttgagtta atacttcaac
    16201 ctctaataat aactctttta tatatcctat taaagttaga ttatgagctc ctccacctcc
    16261 aattaataaa gtatctacat taacctttgg aagtatgaag tttttataat tataatatat
    16321 agcttttgcc gtaaacatag ttactgttgc tatcaaatca tatttatcta catgtttata
    16381 tttgttaagc atgttatcca aataaacttg tccaaatacc tctcttccag tgcttttagg
    16441 tggcgcttga ctaagatact tatgactcat caaatctttt aacatttctt cattaatttt
    16501 accttttgat gcaaagtatc catttttatc atactttcta ttaaataatc tttgacatac
    16561 tccatctatt atcatatttc ctggtcctgt atcaaatgca aatacatcat ctatgttaca
    16621 tgatttaggt attatagtaa tatttcctat tccaccaata ttttgaagtg ctaaattttt
    16681 attgttatca ctatataata aaaattcact atatggtact aatggagcac cttctccacc
    16741 tgctgctata tccattactc taaaattaga tattaccttt gtatttgttt catatgctat
    16801 aactgctggt tcacctattt gaagcgttga agatacaaaa ttattataac ttcttggtat
    16861 atgaaagatt gtttgaccat gagatgcgat aaaatccaaa tttgctatat gaaagtttgc
    16921 tttatgacac actgatttta cagctttact aaataaatat ccaagcttaa aatttaagct
    16981 acagattaaa tcaacactcg attcctcttc tatacagcac tttcttattt cgtcttttat
    17041 atctttagga atttctagag ttataaattc tatttgctta actttagtat ttctaccatt
    17101 cccactaatt tcagctagta ctgcatcaac tccatctaag gaagttcctg acattaaacc
    17161 tacactatac atagttatca tccttttaag tttatagtta ttcctcccca aatcaaggta
    17221 ttaagtttaa taattttaga attaatctta tttcatcttt aattacgata atttactctt
    17281 atgaaattaa ttgagttaat cttatacacg ttttttacct tagctaaatc ttacattaat
    17341 ttattttcag tgtcaatgta ataccatgta attttttttc acaaacaaaa catattctct
    17401 ctccaaaaac catattaaaa tagttactac aaataaaaaa cataccaggt acctatctga
    17461 tatgtttttc atatataata attattattt tataatatag atagagcatc atttaaggca
    17521 ctaattaaat cttcactatt ttcaagaact aaatttattc ttattgtata tgtcgatttg
    17581 tatatgatga tagattttat agtctaataa cctaactatc taggcttaaa taatccgata
    17641 tatcataaaa atagatatta aaaattgtat atgaaccatt ttccgaacat acctcaatct
    17701 catgccctaa actatctatc attttcttta caaaatacat ccccattcca gtagacttat
    17761 acaattgatt tcttccattt ttccccgtaa atcccttgtc aaaaacccta gatatatctt
    17821 cctcatttat acctatacca aaatctttta tagataatac cactccattt tctaactttt
    17881 ttgaattaaa ttcaatctta ccaacttcct ttgaatactt tatagcatta ttaataattt
    17941 ggtctaaaac atatgacatg catttagaat ctgtgtaaac atcattttct aattcattca
    18001 aactaacttc tacattattt tttataagca aaaatgaatt attttttata gacattttta
    18061 tcaatttttc taaattaaat ttatttataa atacatcttc aaacatagtg ttgcttctac
    18121 tactatacaa aatagagttt actaaaaaat ttatctttgc aacctgattc ttaatatcca
    18181 aactatcttc tatgctactt aacctatctg taattatact taaagaagat attggaattt
    18241 ttatttcatg aacccattta gctatgtact catccaattc ttttaatgat tgtttataag
    18301 tctctatgtc actattattt tttattttta agttttctat aatatctttt attagatttt
    18361 cttcaaaaga ttgtccatca actaaataac tgtctatttc accactattt tctatacaat
    18421 tatataaatt tgtgtaacta tttctaaaat ttatataatc tataacaaaa aataccatta
    18481 ttataaacag tactaaaaaa tctaaatata ttaactctga atatttattt ttaaaaatat
    18541 ctatagcaaa taagtagata tttattatac taactacaac taaaggtaga gcaatatact
    18601 tagacctacc tttcaaatat tttactagca ttgtattata tatcctaccc ccttcttagt
    18661 ttttataaaa tcatcaaaac ctagttcttt aactttactt ctcaatctat tcatatttac
    18721 agtaagtgca ttgtctgtca caaactcatc attatcccat agagtcatca taagactttc
    18781 ccttgataca accctattct tattttttaa taataatgtt aaaattttta tttcgttttt
    18841 agtcaactct atacttttat ccttatactt atgttttata atccctttcc ccacatcaaa
    18901 catcaaatct tcacaataca aaatactatt agaatcacta taattataga ctcttcttat
    18961 tataccattt attttggtaa ccaatatttc tatagaaaat ggtttggtaa tgtaatcatc
    19021 tgctccatta tttataccca ttattaaatc catatcagaa tctcttgatg ataaaaatat
    19081 taatggtaca tttgaaatag ctcttatttt ttcgcaccag taaaatccat tatataatgg
    19141 taaatttaca tctaaaagta ctacatctgg ttttttatct ataaattcat ctaatactac
    19201 ctcaaaatca tcaattaatt ctactttaaa tccccatttt tcaagtgcta atgcaatctc
    19261 ttttgataaa tcaatatcgt cctctactaa taaaattcta tacatttgta tattctccat
    19321 tatcctaata tttcactatg ctatgaatta ttaatatcat aacatatttt atatgaattt
    19381 attacccaaa ttatttagtt aatcaacaag gagactctta ttacaagaat ctcctttctc
    19441 tttattaata tatcaactaa tttttataca ctacactctt aaacttatta acagatactg
    19501 ctccatatat tatgaatatt atcaaacata tcactaaact tagtatatat ataccaaata
    19561 aatcggtcat caataaatct cctgctaatc tcaatgcaaa ataactagat atagttgcac
    19621 ttaacattgg caaagtataa aacatagcta cttctttaag tatagctcta tttatatctt
    19681 tttctgtagt acctattttt aacaaaatct tatatttctc tttatcttca tatgcatcac
    19741 tatatatttt catatatatt atacttccag ctacaattgc aaagactaag aataaaaaca
    19801 caagcactac atatgcaaac ttcatccatt gactctcacc ttgagcttta tatccatttt
    19861 gaatataccc attttttaaa cttaaattct tttcaatatt gttgaacatt acttgactat
    19921 tttcttcttg aaataccttt gcaccataaa aatacacttg ttgaccctta tctcttagtt
    19981 taaaatatgt atcatcattt accactataa tactttgatt aagtcctgtc cctagtactg
    20041 gaacttttac ttctcccctt gaaatattat ataactgacc atcaagtttt atcttggctt
    20101 cttttgtagc tattgatgct atcgtttgag ggctttttaa ctctattaca ttatttccac
    20161 taactaatct ttcaccatac tcttttaatt ctttatcatt gccattcact ttaagagtat
    20221 tacaaaaatc actatatttc attatagtat ctgttgtatc ataagcttct tttatatctg
    20281 ttttctttaa aattgttagt tctggattta ctgagtactt tatcttatta gtatctttta
    20341 ttatattctc tatttgtttt ttattcactt catctttatc cattgaaact atgctaatag
    20401 tgtacacatt tctttgttct tgggcattct tatgtatacg cttcatcgca actgaagcac
    20461 caagagttga tattgttgta gttataatta tagctattat agcataagtt ttatagtttt
    20521 tatttaatcg gtagtttata ttgtttattg caattatatt atttccatta aataatattt
    20581 ttttattttt gattataata tcaaatataa caggcataac tgatttgaat aatccataag
    20641 tacctactat tattatacct ataacaggta gtacactagt tatatctcca gttcttatat
    20701 tggtagaaag atagtagcca tatcctatta ctataattga tactatagct aatatatata
    20761 tacctattcc tacctttggt attttttctt gttttttaga tatattgatt agatttatta
    20821 cactacttct acatattgtt ataaaacctt ttacagccat tattataaat atagaaataa
    20881 agattaaagt agcatatagt atagcttcta tactaagtcc attagatacc tttatattaa
    20941 atccagataa ctttattatc actacttgaa aaaatctaga tgttattatc cctataatta
    21001 ttcctgttat acaagaactt attcctataa gcatattttc tacaaaatat atttttccta
    21061 tagtatataa atctaatccc ataaaagaca atattccaat ttctttagac ctattcttaa
    21121 aaaatatatt tgttgcatac catataaaga aaaataaaaa tactgatagt atcattatta
    21181 cagctactaa cactgatagt gtaaattttt tattttcatc ttgtaacaca tttatagcat
    21241 caccatatct taataattca aaattagtta gcacaactac tgaaaaaacc atagatataa
    21301 aaaatgctag ataagttctt atgttactct taaaattatt ataagcaatt ccaagtatac
    21361 tcacttacat ctcacctccc atagaggcta gcatatccat aatttttcca taaaattctt
    21421 ttctactatt tcctttgttt aatttacact ttatatttcc atcatttatc atatatactt
    21481 catttgaata acttgctgtt agagggtcat gtgtaatcat tataatagta gcgttatttt
    21541 ctctgtttat tttttctaat gattctaaaa gttcatatgc tgatttagag tctaaagcac
    21601 cagttggctc atcagcaaat atgactgttg gatttgtaat taatgaccta gctgctgcgc
    21661 ctctttgttt ttgtcctcct gataactgat atggatattt atctaaatga ttttctaatc
    21721 caaatttttt agctatagaa aataccttag cttctatctc cttacttttc ttttttccta
    21781 atgctagagg aagtgctata ttgtccatta gtttcatatt gtctaataga ttatagtctt
    21841 gaaatataaa ccctattttt tctcttctta attttgatag ttcattattt tttacttttg
    21901 atatattaat tccatctagt attatttctc ctgaagtttg tttgtcaagt gtagacatta
    21961 aatttagcaa tgttgtcttt cctgcaccag atggacccat tattgatata aaatcacctt
    22021 cattgactgt aagagatata ttttttaaca catttgtttt aaatcctttt attccataat
    22081 cttttgatat attttttatt tttaatattt ctttcataat aatagcctcc agttgctaat
    22141 tttttaatat atatttattt taaaggtttt atataaataa atccttaagt aaatattaca
    22201 ttttaactgt tagcattaca cttttgtaac atatggaaga ctattataat tatagtagaa
    22261 atatcttaaa ataaaagcat gatttacttc tttgtataaa caaagaaaag aagctcctaa
    22321 gaagcctcta atctatacaa ttattcttct ctataaaaaa ccaaaataca cattgattgt
    22381 ggcaaataca gatactataa acaagtgata ttactactat atattaaaac aattattcct
    22441 gaagataatt caataagact atcaagcaat aagctaagca tccaaaaaga cttaatacca
    22501 ttaatgtata attataccca atattaatgc tcaaaaaacc agcaacagtt ggaataatca
    22561 gagatgaacc tcccataaat atacttaaag ctattaattg gctactactt ctaagttttt
    22621 taggagaaac ttcaaatatt aatctttttg aagtaattaa aattaaagga gtagaaaaca
    22681 cttgaaaaac acacagtata ataatgatat ttgaatcatt tgttaaagca aataatagaa
    22741 actgaactgt tatcataata gcacttaatt ttaataagtt taaactgcta tattttttta
    22801 ataaataaga accagccaga tatgttggta tctctaacaa tgactgtaaa gaccacttta
    22861 aagatatctg agaatttgta gcatttaaag ctatcatttt atctattaca gtacagttat
    22921 ttgctaccac cattgagtat aataagaata aaatacatac tagtaagata tattttttat
    22981 cttttaataa ttcaataata tctttattag ttacatttat tttcttatta atcttttcaa
    23041 tatcaggtaa taatagtata tttataataa gtaatactac aattaaacca atcgctacag
    23101 ataatccaaa atatgagaat ctaagaatca agtaagatgc taccatactt ccaatagccc
    23161 atccaatcga cccaaaagtc ttaataaatg acaaataatt attaatatta tctccacaac
    23221 ctattatcca tgtatcatat aaactacagc aagtatttaa taatcctcct gataaagcaa
    23281 caagtaataa gtatattata aaagttgcag attcagtaga aaacataata actgatgcaa
    23341 ttccataaac tgataaacta attaaaacaa tttttttcat tgtttgatac ttatcagcta
    23401 atactccaaa taataattga aacaatatat tggttattgc atagctcgat aatataattc
    23461 ctctttcaaa cgctgagtaa cctaattttg ataagaaagg tgtgaatttt gttgaagcaa
    23521 aggcaaaaat aatatatgag aaaaaataga atatgataaa tttaaattta atcgctttat
    23581 taatcataat caatcaaaat tgataacttt aataccacta tttccttcct gatttaagtt
    23641 tgaaagatga tattttaccg tattaggatt ctctacaata actaaaggaa tatataaagt
    23701 attttcaagt aacttattat caatttctac taacttttcc cccatattaa cttcttgacc
    23761 ttgtttaact tctaatttaa agttttcttt ttttgttgtt gcactatcca atccaatatg
    23821 aataagaatt tgaacatcat tttgcaattt aattcctata gcatggttag tttgagcaat
    23881 aaaacttatt actcctttag tcggtgattt aagaatattg tcctttgcta tcatagcaaa
    23941 tccatctccc attaccttag ttgaaaatac tttatcagga atttctccaa gattaattat
    24001 ttttccatca atgtatgaat taattgtaat tgcttctttt tttcttttaa agaacattat
    24061 agacactcct ctacataatt aactttattc taattatcat tacaaataac gatattaagc
    24121 atactgcaat tgaggaaaaa agagccttat agctctttcc ccattgtagt tctttaatac
    24181 tgcttatgat ttgtttaatt ccaacaaatt tataaatata ggtaaacatg ataacaatag
    24241 ttattgtgta aatttggaat atctgcccta ataagactaa atagaaagca atcatgtttt
    24301 cttttatctc tttattcttt tttatattgt aaataacata tgcactgtat actgcactaa
    24361 agttagagat taaatttaaa cttgcattaa catcattgtt taaaagttgt gaaaatgaaa
    24421 tatttgtaaa taatgatgtc aattctaaat acactatata taaaacaggt aatattagaa
    24481 aacaaaccat aaccactgaa agaaatatat tttcatcttt taatactgct gtgatatttt
    24541 ttattttatt tgttatcatg tttttgttct ccttatttaa aagttggcca gaaatcctta
    24601 ttagccacaa tcatttcatc taagatttct tttgctactt ttgcacttgg aacagtttta
    24661 ctcatagtaa acgactgcca taatttttga taactttttt ctatccatgc ttctacagct
    24721 aatttttcag aagcaacttg ttgttccatt aaaccttttt ggaaagttgg aattttacct
    24781 atagacatag gttcaaccat atcttttcca acaaggcaag gaatttcaac cattgcatca
    24841 tctgcaaaat tttcaatagc tccattatta ggaacaatca gtaaaaatct ttcctgagta
    24901 ttaaatgcta atgcacaagc taattcaaca ataaattgag aatgttcact tgcatcaaac
    24961 catgtatctc ttgctgtgcc attttttata attctttcac attcttcgaa tgtatccttt
    25021 tctcttctat ccataatttc atttgctctt gtataatttg gattagcatg ttcaacagtt
    25081 tcatcagcca ttaaataata ttttaaataa gtattaggta atgtagtcgg ttctatttcc
    25141 aataaatcct ttgctgctct catagtttct aaccaactag attctttgtg ttgaccttga
    25201 ggtgtaaatt cctcatatcc ttttttagca caatattcct gtaatttagg cattaaatca
    25261 tttccttctt tgtccttaat acttgtccac cacccgaaat gattcaatcc ataatatcta
    25321 acatcaaagt ctttacgaga atttaaacct aaaactctag caaatatttc ctctaaacat
    25381 actggcatat cacaaatatt aataattctt gaatttggtc ttaatacacg acatgcttct
    25441 gcaactatag ctgctggatt tgaataatta agcatccaag catttggaga atatttttcc
    25501 atataatcaa tcatttcgat tattggacct attgttctta atccataggc aattccacct
    25561 gccccacaag tttcttgacc aacaacccca tattttagtg gaattttttc atcttgttca
    25621 cgtaaagcat ataaaccaac acgaatttgt gccattacaa aatcaatatc agtaaatgct
    25681 tcttctggat ttgttgtata actaaattta atttctggat ttttctctct aacaattatt
    25741 gcacatgctt cagcaacttt tctttgtctc tcatcattat tatcatataa tttaattgat
    25801 tcaataggaa atttagcaac actttcaatt aacattgtta caattccagc agtataagta
    25861 ctacccccac cagcaattag tatcctctgt ctttttcttt ccatctttcc ttctccttta
    25921 taataaattt tcaaattctt ctcttacact tggtactgaa agcccaataa taatctgaat
    25981 agcttttcca ttatgcacta aaccatgtgc acctactgcc ttaaaatctt cttctttaga
    26041 aataattgag tcatctttca aggttaatct caacctcgta gcacagtttg taacatctac
    26101 aacattttct gctccaccaa ctaattttat gtattcagat gcttgttgag aggctttact
    26161 caatttattt cctttttcat ctttagtttc taagtattca tttttagtat atagttttgt
    26221 ttcttcacta tcttcctctc taccaggagt tttcatatca aacttcttaa ttaagaaagt
    26281 aaatacaaag aaccagataa atgaaaatat aatccctaca acaacttgaa caacatatac
    26341 cttccagtga tacattccaa gaggaagcca atttaataca acccagttaa taattcctcc
    26401 accaaaatca ccaacaactc ctagggcaaa cgaaatcgtt tgcaagcatg catctaaaaa
    26461 tgcatgtact aggaataaaa gtggtgctac aaataaaaat gtaaattcta acggctcagt
    26521 aattccagtt aatactgcag taagagtagc aggaatcatt aaacctaata cttttttctt
    26581 tttttctggc tttgcagtta catagaaagc accaaataca cctaatgaac caaacacctt
    26641 cgataatccg gataatgaaa atccgattgg atacatttcc tttagagtct tagctgaagt
    26701 ttgaaaatcc tgaatatgag tagcccaata tactgatgta ccacctggaa caacaacact
    26761 atcataacaa attggtgcat atacaaaatg atgtaatcca gttggtatta aaatcttttg
    26821 taagaatgca taacaccaaa ctcctatatt tccactattt ataaaaaacg tttgcaaatg
    26881 tctaataaca ttttgaatat gaggccaaat aaaacaagtt aataatgcaa ctggtatcat
    26941 aacaaagaaa caaattgcaa ctacaagtga tgagcctcta aataatccaa taaaatctgg
    27001 cagttcttta tcataaaatc tattatgaat ataaatgaca atacctgata ttaacaaagc
    27061 accaaccata ccagtatcta atgttttaat accagcaatg cttacaagtc cacttccagc
    27121 acttatttca gctgcgaaat caactccaaa gctttttccc caaaaactta acatagaacc
    27181 tacaaaataa ttaaaagtca aatacgttgc taaagcctcc atacaagctc ttgcttgttg
    27241 cttttttgca agtgctattg gtaaagaaat agcaaataat aatggcaatt gattaaatac
    27301 catccaagct ccactagata ttacacccca aaattggtac caaatatttt ctggtgtagc
    27361 aagtgaaccc ataattgact gattttgaaa tacaattgag aatccaacca ttatcccagc
    27421 aaaagcaaat aatagtgttg gtgtaaacat tgcccctcca aaacgctgta ttttcttcat
    27481 catataaatt actcccctcc tcatattttt agtttatctt tggccttaga tatctattat
    27541 tttaacaggt taataaatat atacaatctt tagggtacat tcggagctta ttcacagtca
    27601 aataagtttt tgcacatttt tatgttcaaa ttctcataaa aaaaaagaat gactacaatt
    27661 tgcaatcaat cccttctgta atcattctat gccttttata aatactatat ttaataaata
    27721 ataactcaat caatataaaa tataacattg tcaatttata actagggtgc atttgtaaaa
    27781 atgagatatt agtcgctgaa atataaattg attcatcact taaatcagtc aatgtattat
    27841 ttttaaactc tgtaattgaa attattttag ttcctattaa ttttaatctt ctagcaatat
    27901 taagtgtatt ctcattctca ccacttaggg atataataat cataacatca tttttactga
    27961 caatattgta aaaagaatca tcaaatgttc ttccctcaaa atcatatatt atttcttgac
    28021 atgataagaa cattctcttt atctgttttg caaccacatt ttgaatatca cctgaaccat
    28081 aagcgtatat tgttcttgat tcataaagaa gtttacacaa tgcatcataa tctttatttt
    28141 tttgatcttc aacatactta attattgaat tacatgcaat atcaaatgac tgattttcta
    28201 tgacatgaac cttttctgat tcccagctta ataatacttt aaactcactg aaacctttta
    28261 aatctaattt tttaacaaat cttgtaatag ttgcacgaga cacattacat ttcatagcaa
    28321 gttcatttat tgacatctta ctacaatgtt ttttatttgc agttatatat ttccatatta
    28381 ataaatctgt atcattcaat ttattataat ttaaaaaaat catttcttct aatctcataa
    28441 actattaccc cctttaatta aaaatcgtca taaatattat accaatattc aacattaaat
    28501 tacagcgtat tcttatattt actatttagc ccataaaatt gactataaaa aatactgcta
    28561 taattgatgt ttaaaaaaat gtagttttaa ttttattctt ttatctaata attaatattt
    28621 tcaagttcta gtgcttttct tatacaatta tcatctttaa aatcagcagt ccttttacaa
    28681 tcttttactt taaattctgg aatagtttta aattcctcat cacatattcc attcttattt
    28741 aaatacatac aacttgccat tctaactatt agaccactat tttttaaatt aaacaataca
    28801 gggtctatac accctccatc tccgccagta gtttcaccaa ctaaagtcgc aaatttattt
    28861 tctttacaaa acattgcaaa tgactctgat gatgaatata tttttctatc aacaagtaag
    28921 tatatattac ccttaaattt tgaatcatct gtagatttta tagtatagga agtatcttca
    28981 aaatcattaa aatcttttat aatttcttta ggtgcatttt cctttacact tgctggtaaa
    29041 ttttctacag gatttaattt tatatttctt gcatttgtat atttttttac tatttcatta
    29101 ttatttctat aaattctata accatttatt ttgacatcat tttttatgag tttagatacg
    29161 attccctgcc aatacctatc acttccacct aaattacctc ttatatcaat aattaaagct
    29221 ttatgttttt ctaaagtgtt aatataatct ccaattattt ttaaatcatt atttatagag
    29281 ccattctttg atgacattga tggtaaatac atatatccaa tctgaccctt aataacatcc
    29341 tttaaaatta attctttttt tacaaaaata tcatctggca tttttatttt aagattcata
    29401 gagtcatatc tatccacaac ttttttatca ttaagaaaat cataccaatc attattagag
    29461 tatgatttct taaaaagttc atatcttttc ttattatcta ttacttctgt gtgtttatta
    29521 tttaaatcag ataatataga tgacagttct tctataaatt cttcatcatt ttcagtgttt
    29581 tttattcgtt ttgtatattc atccttattt gctaaccaat caatattatt aagcctctta
    29641 tttacaccta gatatggata tcctgtcttt acaacattat acacataatt aaagtcatcc
    29701 accttttcct cttcagacaa ctgattcttt tgagaataag tagaattacc taataatatt
    29761 ataaatgcta aaataattac taaaaataat actttctttt tcatgtaatt ctcatccact
    29821 ttcagatttt atattttaat acattgtatc attattttat tatcacctaa acagttaaaa
    29881 gaaattcttg aagatatatt ccactattat tctctttaaa aatttttatt tgtattttca
    29941 aaattatttc atgaaagtta taaaataaca attatatt
//
LOCUS       DAYAPA010000040        29962 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.040, whole genome shotgun sequence.
ACCESSION   DAYAPA010000040 DAYAPA010000000
VERSION     DAYAPA010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 29962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29962
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.040"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            53..2077
                     /locus_tag="ACGI59_001650"
     CDS             53..2077
                     /locus_tag="ACGI59_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFG9739439.1"
                     /translation="MDKKKLILITYDKLNSDHYKEELTNFFGDEIIIETQNILDGIKE
                     NLEGDVVLSLSPLTSNFLIKHFKEDIEIIHGTKALSKLGYEKMMKLPPGTKSLLMTTN
                     KTSAFEMATYLYKIGINHIDFVPTYPDCDEIYDLDTAITPGQIRFIPKYIKNIVDLGW
                     RKISLDTYMSLLVVLKLKNEKFIEKLYKLSKETLSHDFLNTSLDNISKLKTILYMTID
                     EIGDGLIFFNTFNKVTFVNKSLLNMLELDEKLIKSPSLMEYMPKSFLDKITKNLNIDN
                     MIIYIDEIDKKFILSKKPFYLYKNIEGCLITLKDVNNIEILEQKIRSDSVKRGYVAKY
                     KFNNIIGNSSIIKDCIKKAKKMALTDNPILITGETGTGKEAFTQSIHNHSNRKNKPFV
                     AINCASLPSELLESELFGYEDGSFTGAKKGGKKGLFELAHTGTIFLDEIGDMPHDLQV
                     KLLRVLQEKEIRKIGGTSIIPIDVRILAATNKDLEKLIIENKFRMDLFYRISMFTLDL
                     PPLRKRLEDIPLLLESFLKELPYKNIKLDKSLLEALNSYTWMGNIRELRNCVEYMAYM
                     GSNYLTINDLPQNISSKLNNNHMSSNMSIFNDLNQYDKNICISILKSLHMKPMGRTKL
                     MKFMEYNVTEYEVRNMLEYLTRNGYLISSKGRKGSSITEKGIKIIENNII"
     gene            complement(2307..3494)
                     /locus_tag="ACGI59_001651"
     CDS             complement(2307..3494)
                     /locus_tag="ACGI59_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3798 domain-containing protein"
                     /protein_id="HFG9739440.1"
                     /translation="MLRKITAFMLSICLIIGLTGCSLLVGKKDKEKIIDDFKVAVVTQ
                     PLSENKVQYNMVEEMAKEYEEENKIDKDKDGQTKVKQTIKHVVLPENFTSNIDSAINK
                     IVKLADDKEVQAIVVSTDQAGLLPALQKVKEKRPEIITISAPMGDDKNQLSQFVDVNL
                     GVSAEERGKVLAERSKEMGAKAFIHYASTDDLKDVNIAKRLEMIKETCKNIGLPFVQV
                     NTPNINTEEDKNKVKQFLNEDIEKQVKKYGKDINVFGVNEYMDEVILTKALELKYIVA
                     EQSNPSPIQTYPSVMGLKISEKDAQNYDKINDMISEKAKAFGMSNRLGGYPMPMDAFL
                     PSVAIYLATEMVKQDLTQEDVCDPDYLEAFTELRFGIGSEFTPLTEVLYNYQSVILSQ
                     LIY"
     gene            complement(3696..4967)
                     /gene="brnQ"
                     /locus_tag="ACGI59_001652"
     CDS             complement(3696..4967)
                     /gene="brnQ"
                     /locus_tag="ACGI59_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFG9739441.1"
                     /translation="MRDIFIVGFALFSMFFGAGNLMFPPFLGMESGKDWLIPLIGFVV
                     ADAGIAMLVIIATARCKGSMDDVLRRAGKGLARVMSVAALACLALLVIPRTCATTYEM
                     GIMPIFGDHGPVAKAVFSIIFFGLTLAFTIRSSKVIDIIGKYLTPALLLVLFVLIVKG
                     IISPAGPMSPEHMIDKNLFGEGISQGYQTMDALGAASMATIMLMSIIAKGYTSDKDQI
                     GMTIKAGFVACVFLAVVYGGLTYLGATVSTLYDTSIPQASLLVEITSILLYGQVGKVI
                     LGVIVALACLTTSTGLTASISTYFEGISKASYKQLVIGICVASMLISNLGVDSIIAIS
                     VPILQTIYPVLLAIVVMELAGKHIKNDNAFKGAAYVTLVISLLSAINGMTGAVPFIQS
                     LPLAGLGFNWVIPAILGGIVGNFIKSNKQVA"
     gene            complement(5108..5761)
                     /locus_tag="ACGI59_001653"
     CDS             complement(5108..5761)
                     /locus_tag="ACGI59_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFG9739442.1"
                     /translation="MTKIIKVQKPKFTGELEIIENLEDILEDIFNDEKIFNKIIENEV
                     ISDLDSVRVSFESCIFRNVSFENCNLKKIDLLDVVFENCNLSNTFFDGGSIYRVEFKN
                     SKLIGVRFDDCILKNVLFKDVLGKYSNFSFSKFKIVSILDSNFENSVFQEVKGDNLVF
                     EYTNLKKANFNGTKLSGIDFTTNNIEGIEIGIDDIRGAIFDVSQAIDLTKLMGIIIK"
     regulatory      6090..6191
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            6264..7607
                     /locus_tag="ACGI59_001654"
     CDS             6264..7607
                     /locus_tag="ACGI59_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFG9739443.1"
                     /translation="MVPNTNNVQPGGNNSSLSFLDKMFKFSERGSSLKTEVIGGVTTF
                     LTMAYIVFVNPAILSEAGMDKAALITVTILATAIGTLIFAILGNAPFALAPGMGLNAF
                     FTYSLVIGAGIPWQTGLGVVFLSGCVFFTLAVTGLRKTIADAIPRELAVASSAGIGLF
                     LAFVGLKNMGVIIANPDTIVSLADFTPTVLIALFALILMAILEYKKVKGGILISIVVA
                     TIISVIFGFVELPTAIISTPPSIAPVAFKLDIVGALKFSLIGPIFSFMFIDMFDSLAF
                     LITCCKQMGLEDENGEIKDLGRMLYADVSSTLIGSFLGTSTVTTFGESASGIAAGART
                     GLASIVTAALFLATLIFTPLLGIVPTYAAAPALVMVGVFMFESIKQIDFADSKIAVPA
                     FVTVLLMPLTYSISIGLCFGFISYIIMHVVAKETDKISITLWIIGLLAVVNLVLS"
     gene            7786..9150
                     /locus_tag="ACGI59_001655"
     CDS             7786..9150
                     /locus_tag="ACGI59_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HFG9739444.1"
                     /translation="MSQLLIKNAYLITMNADREVYENGSILIENNIIKEVGKFDINIV
                     DKDAEIYDAKGKILMPGLVNTHVHLSQQLGRGLADDVVLLTWLRERIWPYESSFNYED
                     SLISSTACCVELIKSGVTTFLESGGQYVDAMVEAVDKTGLRACLAKSVMDTGDGLPEA
                     WQKTTDEEINTQLDLFHKFNNTSNERIKIWFALRTIFNTTEELLVRTKELADKYNTGI
                     HMHIAEIADEISFVKQNNGVGTVEYLDKLGVLGPNLLAAHTVWLTHKEIDLFRLHDVK
                     VSHDPGSAMKVVLGFASIPEMLEKGIPVSIGTDGAPSNNRMDLMRDMYLTSLIHKGRT
                     LNPTVVPAEEVLEMATINGAKCALLEKEIGSLEVGKKADMIILNPDTIHCLPMHNPIG
                     NIVYSMTSENVDSTICDGKWLMKERKVLVVDESELLNKVKKQASKIKDKAEVKIPSKF
                     KIIK"
     gene            complement(9328..11193)
                     /locus_tag="ACGI59_001656"
     CDS             complement(9328..11193)
                     /locus_tag="ACGI59_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIRAN domain-containing protein"
                     /protein_id="HFG9739445.1"
                     /translation="MENVFKRLQEFNGYDGYKESFEMNYLCIYESIPLREQVELANNL
                     VDEILNMYKSESNEIYLLEGSNSKSLICYFEIFMKKINTLVKEMIIDEKWLYKLTKEL
                     IYKSKKVEYVKLGLVLSEKYLNVENLREVVDTFSKSGEYVFYLSNTIKKLEFYNTYLF
                     NLSKKATGSIKVFAIVNMENLDSKINSYLIEDGYKDTKYERLLMNYIISIVDLNEYLE
                     KRDLDKEKINNLARLICNYLLSVEFKYIGNKLELVNRFLPTVVNYGTNFESLYSIFLI
                     AINVLKDENIECNKIEFEKEINGILLSEKWKNIYFEALRDASGKTEDIIKMSEIYDVN
                     LSFDDLLPYLNRDIRDFEVYWHISKKGTTSSRLKLLNFFEETFKIDDLIGKMKDIEKD
                     KLTQEYYDDMLFFIVLKGSKSLYPEGKNISLKGIFGNINEVRKESINILKRYREKLSL
                     EELKIVKEAYEKEKNVILKDELRRVLYESNNLKKEFVNIEKIKVDEHGKDIYLTSIAV
                     AGSRFRNREYLEKELEKSKIYYLTREKDNLYDEKAIKIVGETGYVIGYVPRKENYILS
                     NLLDGGKLLYCRVTEYNLYEDCIYANVYLSYKDVIETVENSLKMVLDKSRIKLIN"
     gene            complement(11429..12646)
                     /locus_tag="ACGI59_001657"
     CDS             complement(11429..12646)
                     /locus_tag="ACGI59_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFG9739446.1"
                     /translation="MNNKKYYPTAIALYFSYFLLGIGISILGQYKPEFSSMWGAKTLS
                     DGTLDVSIVLAVIAALGLGRLISYPFAGPISDKYGRKVSGLIGNFLHAIFFVGIVFSP
                     NFYIAYVFAIIGGAANSFLDTCVTPSCMEIFASLGTIANMFTKFTIALAQFLLPFIIG
                     FVAANSISFKVIFIVTAILIVVDAFLIAVLPFPPANNVIDNKGKTVKSEKMKFTPTSI
                     ALVCIGFTCTSTFVLWLNCNQELGKLYGMADPTKIQSFYSMGVICAVLITSLLIKKYI
                     KPIRILVIYPIIALLMLLVVYFVQTPTICMLGGFVIGYSAAGGVLKLTTSTANEMFPT
                     NKGKITSIVMIASSIANYVILNIAGIITKSGGVNGPKYVVLFNVAITFVGILLALFVN
                     MRYEKEKVYDYDV"
     gene            complement(12846..13709)
                     /gene="aroE"
                     /locus_tag="ACGI59_001658"
     CDS             complement(12846..13709)
                     /gene="aroE"
                     /locus_tag="ACGI59_001658"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFG9739447.1"
                     /translation="MEISGRTGLFALIGTPVGHSKSPVMYNYSFKKLDLDYRYLAFDI
                     TVDKVKEALLAIKTFNIKGANVTMPCKSAVTEYMDELSPAARIIGACNTIVNDNGKLV
                     GHITDGVGYVRNLKENGVEVKGKKITIMGAGGAATAIQVQCALDGAREISIFNPKDDF
                     YKRAEQTVENIKKDVPECVVNLYDLEDTNKLYEEIQSSDILTNATLIGMKPYDNETNI
                     KDTSVLRKDLVVTDVVYNPKKTKMIEDAEANGCKAIGGLGMLLYQGAEAFNLYTGLEM
                     PVEEVNELCFK"
     gene            complement(13791..15713)
                     /locus_tag="ACGI59_001659"
     CDS             complement(13791..15713)
                     /locus_tag="ACGI59_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFG9739448.1"
                     /translation="MKISSMFTPIRIGSMTVPNRFVVPPMGNNFANTDGTLSETSKAY
                     YLERALGGFGLITIESSVVDKKAKGGPRKPCLYDDSTIDSFKNVIDACHDAGSKVSIQ
                     LQHAGSEGNEKVAGHPLKAASAIPASNGRNTPLAITTEEIYELIESYGDAALRAQKAG
                     ADAVEVHCAHGYLVSSFISQRTNKRVDEFGGCFENRMRLPRLIIENIRKKVGNSLAIL
                     CRINSTDDVPGGIDVHDSSVIAAYLEDCGIDGLHVSRSIHIHDEYMWAPTTLHAGFSA
                     ELVTEIKKAVSIPVITVGRYTEPQFAELMVRQGRCDLVAFGRQSLADPEMPNKAKNGK
                     LDEMIPCIACLQGCVPNMFQGKPIACLANPILGHEAELKPAEISKEVLVVGGGVGGML
                     AAWVCAKRGHNVTLVEKSEVLGGQMRLAAYPPGKGDITNLVRSYISKCNQYGVKICTN
                     TEATVELIKEKSPDVVIIATGATPLVLPIPGINDSGLIHAVDLLDGKKSCGKKVLVVG
                     GGMVGCEVAAFLGEQEHEVTVIELREEVGADVISEHRKFLMNDFENYNIHTITGAKVS
                     KFYDDGVDYLLTDGTEHDLKGFDSVVLAMGSRNYDPLSEVIKEVVKETYIVGDAVKAR
                     RALDATKEAFEVALNI"
     gene            complement(15777..16625)
                     /locus_tag="ACGI59_001660"
     CDS             complement(15777..16625)
                     /locus_tag="ACGI59_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFG9739449.1"
                     /translation="MNNQKNLNIPITISSYTLGTEVSFRDRVRIAKEAGFDGIGLRAE
                     NYIDAKNAGVTDEEMIAILNEYDMKVTEVEYITQWGREEDRTEAQREKEQNVYHMAHL
                     FNVKHINCGLLEMIPEEQIITALGELCDRAGELIIGLEFMPYSGVPDLATAWRVVKGC
                     NRENAMLILDTWHWARAKQTAEDLAPVPADKIVSIQVCDVLETPYEKLRDESLHDRLA
                     PGEGYGNTEEFVKIIKEHNISPRVIGAEVISDSLISKGLSTAAETVFNATKKVLDKAW
                     SEVSPK"
     gene            17038..17919
                     /locus_tag="ACGI59_001661"
     CDS             17038..17919
                     /locus_tag="ACGI59_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFG9739450.1"
                     /translation="MNLYHLRYFVTLAHLEHYTKAAENLSITQPSLSHAISLLENELG
                     VALFEKEGRNIVLTKYGKIFLKDVEKSLEILDSSVKSLKITGTGEGQIDLAFLRTLGT
                     DFIPDIVHKFLKSNPAKSIDFKFHTGVTTDIIQGLKERKYDIAFCSKLEKEKGIEFIP
                     VAKQDLVLIVPYSHPLAAKDTIDLKETIPYPQIVFNQRSGLRYIIDDMFKKINQQPNI
                     VYEVEEDQVIAGLVAKNFGIAVVPNMNMLSFTKVKVIQIIHPSWERNFYLAFIKDRYL
                     PPAIKNFKNFVIKNAQL"
     gene            complement(18127..18966)
                     /locus_tag="ACGI59_001662"
     CDS             complement(18127..18966)
                     /locus_tag="ACGI59_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HFG9739451.1"
                     /translation="MSKIIKKKYIMFIDESGRADINHEDPFTLTGVIFEYKYAVSQGD
                     YVCSLRKEMDAFKEYCFGTSNIGIHLTDISRKSRAFEKFDDSQIKLFYDELPLFLSRL
                     ECTIISVTVDKARLKKYYAPSKEPYVVAFIHVLQNFYSFVNNETVESARIVIESRDDV
                     SNLKVQKAFFDVFNNGTIYLDIDEELRDKVKGFIIAKKEDADYREGLEIADILCNPLS
                     RARRGLIEANPKCMQYGEKNKIFKSVRNKIYTPTSVNDIRNWGFKEVPIVEPVGPWLE
                     KMR"
     gene            complement(19145..21079)
                     /locus_tag="ACGI59_001663"
     CDS             complement(19145..21079)
                     /locus_tag="ACGI59_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp22"
                     /protein_id="HFG9739452.1"
                     /translation="MKKSLKKYLVLALTLVLVFACGVPESSAASKHVIIVNSRKNTLG
                     YFVNNKLVKEFRVATGKKGSETPTGKTKVVNKIKNRPYYKGNIPGGSPRNPLGDRWMG
                     LALKGTYGDTYGIHGNNNESSIGKHISGGCIRMHNKDVRWLFDQVPVGSDVIIDYSND
                     SYVKIAAKYKINLNQTGWKTENGKKYYVKSDGTYQKNSWLKVNGKMYYFDASGVMQTG
                     WKTINNKKYYLGTDGARVSGWKVIDGKTYYFNSDGVMQTGWQEKNGKKYYLGSDGLAV
                     TGWQEIDGNKYYFDKTGIMQTGWQQIDGKSYYLDKDGKMLTGSQKIDGKDYTFNEDGT
                     INPTWDTIIGANRFDTAKKISSVGNWNADSSDTVILVNGNAIADGITATPLASSYDST
                     ILLTNTANLPTETVEEMKLLAPKTVILIGGENAISSKLEQEIKTTFNTETKRIAGQDR
                     YQTATRIAEELGNREEIKTAYMVSGNGEADALSVASKAGEEKQPIILVNKDGITEESY
                     KWLTERKLENAYFIGGPSAINDSVIAKMNDITTEDISGNRIYGDSRVDTNAKVIEKFY
                     GDTDLQAVLVSKSDALVDALSAGPLAVKLHSPIVLMDNSGLSSEQQRVFANKKVETPY
                     QIGGGVSYIVMDKLMDILAK"
     gene            complement(21191..22204)
                     /gene="galE"
                     /locus_tag="ACGI59_001664"
     CDS             complement(21191..22204)
                     /gene="galE"
                     /locus_tag="ACGI59_001664"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFG9739453.1"
                     /translation="MAVLVAGGAGYIGSHTAIELLESGYEVVIVDNLSNSNSIVVDRI
                     KELSKKPVKFYNIDIRNKDEMHVVFKENNIESIIHFAALKAVGESVEKPIEYYSNNLI
                     STLNLFELMREYGVKKFVFSSSATVYGDPHTCPILEDFPLSVTNPYGRTKLMIEQMLV
                     DISKADKSLDIALLRYFNPVGAHKSGRIGEEPNGVPSNLMPYITKIAVGKLKELSVYG
                     NDYPTHDGTGVRDYIHVLDLAAGHVKALQKLEENPGLVVYNLGTGKGYSVLDLVKAFS
                     KASGKEIPYKIVGRRAGDVAMCYADSSKAEKELGWKAKYELEEMCEDSWRWQSMNPNG
                     YEE"
     gene            complement(22228..23103)
                     /gene="galU"
                     /locus_tag="ACGI59_001665"
     CDS             complement(22228..23103)
                     /gene="galU"
                     /locus_tag="ACGI59_001665"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFG9739454.1"
                     /translation="MQMTVKKAIIPAAGLGTRFLPATKSQPKEMLPIVDKPTLQYIIE
                     EAIESGIEEILIITGRNKKSIEDHFDKSVELELELEQKGKTEMLEMVRDISNMVNIHY
                     IRQKEPKGLGHAIYCAKSFIGDEPFAVLLGDDIVDSDVPCLKQLIDTYNEYKTTVLGV
                     QKIAKEDTNKYGILDVKHIEDRVYKVKDMVEKPAIEEAPSDIAILGRYIITPAIFSIL
                     EKQTPGKGGEIQLTDALQTLGKQEAIYAYNFEGRRYDVGDKIGFLEATIDFALKRENL
                     KDDLMDYMRKKVSEK"
     gene            complement(23295..23615)
                     /locus_tag="ACGI59_001666"
     CDS             complement(23295..23615)
                     /locus_tag="ACGI59_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9739455.1"
                     /translation="MSSKYNVWTFNYEFLGLDSGDEKNNEVYYEIDLDEILNENYIDE
                     DIDSGLDSDGNSILGELISEMDIDEDVSVDMTYQELEVDLEDLDSYLDEDIDSDIKGI
                     LDEI"
     gene            complement(23605..25596)
                     /locus_tag="ACGI59_001667"
     CDS             complement(23605..25596)
                     /locus_tag="ACGI59_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9739456.1"
                     /translation="MLKLGEKIIYELSDGFELSGISRYSKEYKKLHITNLGNIIISKS
                     DEVNEDTGVKYIYSFSDNKEKLEAVLEDSQIIVYGNNLPFYIKSENSSKNVTANLTMK
                     ITLDEYDLICKSQREFIFYLKDNMTILSLDDNKFYMGGINKDNEKFIFISGKNRFEIN
                     FDDIERYILEDKRVSLKGYFHMEREGIIVRSVSIFNNNIDSVVPSDLNERVKDNQKIG
                     NLPKDCEIVFCKISGNIDGFDYKNTNMLLVKYQDQLIFINKKSKKTIVKSAKDNCSKL
                     NLGEDIILYDNKNVFNLHINDKNREIMQIDDLKDIENEIVGYTLKHAPFFIQEDFDSL
                     TILKSFQKEIISIKNSDIKDIVINKELENENSNFVETEIKFNNQKVLLNLSKSMVQKL
                     MQDVFIYAKQPLLKENSIEVIYKNWSKAMNDMIIFNFFGNIYYMKSEFDKILEKELND
                     EIRIEVINSLYKQIQEQRNNLDLLSAYMPRILENQEIDLFEKYNTKLDVQVFKQIKNL
                     LSDLSYNISSYLNEVEKSLDNIIFVISGEDKKKYNYRMLKESESASLDVFLKQAISRL
                     NHLVENMYPYYVDETSREMFKLFELLWKNYRNIDDDSIKEILFERITNTYVFKQLTLN
                     NSTKERRKDIIEKIYNSVDYGTNKLDENMFFTGGIKYVK"
     gene            complement(25719..28262)
                     /locus_tag="ACGI59_001668"
     CDS             complement(25719..28262)
                     /locus_tag="ACGI59_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HFG9739457.1"
                     /translation="MKVLSENNIIRNTVLNVTASFLKQESKINEKLDGVLEKKFEKVE
                     FNEAKYAELLKFNILFYKTLARNTEPLIGKWIVDKYIPEIDELEKDLELTTAKCRKYV
                     NKAMKEGLDSLKANDLNSFLAYDKMELSERRRRLEKDYKVLNLYKDLLNISLRKISLE
                     KKECGDLFLKNQADAKRELKREIIFCVNKILASNKDVVPNNVEENNEQNKVEIKATEI
                     EDLQLGKDNSENDMLLNEIKNSKVKHESKSNDIESKFIDKLNSLNNESISQTIHNEYK
                     KLCGLEELHSVEAYGFGKEIIKDFACATVVLEFLKRRNRDLIEGAMRLTIIGEFGPEN
                     FKEFVDYVIKNKTEISEDTWNKAQNLIKDNYSELENHEIASKRTRRNKDIDIEEYIYM
                     IKNADKDICFRSSISIEDDAEEGTKEEVDSNNQDIGDVVEDKDTTDKEYDSNKEDIIE
                     PENKKSKKKAKLFGFIKKDNEEAEQEEENLNDISPDIILDKPVENHQVKSEEIEQNEL
                     KEIKQEEPSQHIEEERSVKIEKPINNNLDEKVSSNNESKLEKESKNLEDKKAKEIKEE
                     KLENEKSVVIPIKKKENSNKKSKNSSKDKYRENKKEMKNYVSDKEDSLDDEEVVSKKS
                     RLKETIIAVVIVVIVGVGYFITVGNNKKNDKENIPKSSTQQQANNKLTEEEKKAQAEK
                     EKKEAEEKAKAEKEAQEKAQAEKKAKEMEAYKDGKGVYYTVYAGSLKVEKTAKETAKE
                     YEAKGISSTIIQENGYYKIKIGDYSQYGEAQEKCNELAKKSIDTYIAMYDKYYDYKLE
                     ELKESAPSLSAEELKQKYEDLRSELKNKSGYREYVKHLDKLYEEIVEGA"
     gene            complement(28540..28874)
                     /gene="rnpB"
                     /locus_tag="ACGI59_001669"
     ncRNA           complement(28540..28874)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACGI59_001669"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            29006..29683
                     /gene="srtB"
                     /locus_tag="ACGI59_001670"
     CDS             29006..29683
                     /gene="srtB"
                     /locus_tag="ACGI59_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase SrtB"
                     /protein_id="HFG9739458.1"
                     /translation="MKKLYRIVINIILVLVILYSGFNIYSKLTKYNHDTKISSELQKK
                     EYKKEDLSKINSDFKFWLSVENTNINYPVVQSKDNSYYLDKDFYKKDSISGTLFMDYR
                     NKSIDDKNIIIYGHNMKNKTMFNNLNKFKDADFFKKNNKIKITLNGKEFLYDVFSAYI
                     VESDYDYLKTNFNNESDYQNYINDITSKSLYKSPIKVNSNDKIVTLSTCTYEFDDARM
                     VIHGRLI"
     gene            complement(29843..29917)
                     /locus_tag="ACGI59_001671"
     tRNA            complement(29843..29917)
                     /locus_tag="ACGI59_001671"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(29883..29885),aa:Thr,seq:tgt)
BASE COUNT         9883 a         5084 c         3417 g        11578 t
ORIGIN      
        1 cttatacttt aaatacttta aaaataaata ctattttgtt aggagaaata aaatggataa
       61 aaagaagtta atccttataa catatgataa attgaatagt gaccactaca aagaagaatt
      121 aacaaacttt tttggagacg aaataatcat agaaacacaa aatatacttg atggaataaa
      181 agaaaattta gagggagatg tagtgctttc tctatcacct ttaactagta attttttaat
      241 aaaacatttt aaggaagata ttgaaattat tcatggaacg aaagcacttt caaaacttgg
      301 atatgaaaaa atgatgaaac tgcctccagg tacaaaatcg ttacttatga caaccaataa
      361 aacttcagca tttgagatgg ctacttatct atataaaatt ggtataaatc atatagattt
      421 tgtaccgaca tatccagatt gtgatgaaat atatgattta gatacagcta taacaccagg
      481 tcagattaga tttataccaa aatacataaa aaatattgtt gatttaggtt ggagaaaaat
      541 ctcattagat acatatatgt cattattagt agtattaaaa ctaaaaaatg agaaatttat
      601 agaaaaatta tacaaattgt ccaaggagac actaagtcat gatttcttaa atacatcatt
      661 agataatata tctaaattaa aaactatact ttacatgacc attgatgaaa taggtgatgg
      721 tctaatattt ttcaatacct ttaataaggt tacttttgta aataagtctt tacttaatat
      781 gttggaacta gatgaaaaac ttataaaatc accttcctta atggaatata tgccaaagag
      841 tttcttagat aaaattacaa agaacttaaa tatagataat atgattatat acatagacga
      901 aatagataaa aaattcatac tttctaaaaa accattttat ctttataaaa atatagaggg
      961 ttgcttaatt acattaaaag atgtaaacaa tatagaaata ttagaacaaa aaataagaag
     1021 tgattctgtc aaaagaggtt atgtagctaa atataaattt aataatatta ttggaaatag
     1081 ttctattata aaagattgta ttaaaaaggc aaaaaaaatg gctcttacag ataatcctat
     1141 attaataaca ggtgaaacag gaacaggtaa agaagccttt actcagtcaa tacacaatca
     1201 ttcaaataga aaaaataaac cttttgtagc aataaattgt gcttcattac caagtgaatt
     1261 acttgaaagt gaactatttg gatatgaaga tggttctttt actggagcaa aaaaaggtgg
     1321 aaaaaaagga ttattcgaat tagctcatac tggcactata tttttagatg agataggaga
     1381 tatgccacat gatttacagg ttaaactttt aagagtctta caagaaaaag aaattagaaa
     1441 aattggtggt acaagtatta ttccaattga tgtgagaata ttagctgcca caaataaaga
     1501 tttagagaaa ttaattatag aaaataaatt taggatggat ttattttata ggataagtat
     1561 gttcacttta gatttacctc ctcttagaaa gagattagaa gatatacctc ttcttttaga
     1621 atcattttta aaagaacttc cctataaaaa cataaaactc gataagagtc ttttagaagc
     1681 tttaaattca tacacttgga tggggaatat tagagaactg agaaattgtg ttgaatatat
     1741 ggcttacatg ggttccaatt atcttacaat aaatgattta cctcaaaaca tatcttcaaa
     1801 attaaataat aatcatatgt caagtaatat gtccatattc aatgatttaa accaatatga
     1861 caaaaacata tgcatttcta tcctaaaatc attgcacatg aaaccaatgg ggaggacaaa
     1921 gttaatgaaa tttatggaat ataatgtcac agaatacgaa gtaagaaata tgctagaata
     1981 tttgacacgc aatggatact taatttctag taagggaaga aaaggctcat caataactga
     2041 gaaaggcata aaaataatag aaaataatat aatttaaacc tatagtaatt catctttact
     2101 ttatcaaaat ttatatataa ttataatttt ttatttttct ataaattttt tataaaataa
     2161 taaaacttat ttatattaaa aacgaagcta tctcaacata tggataagct tctttttaat
     2221 tgttaaaaag ataaaaatat atttgattta acagaaaaag agtgcctcat aaactaaaaa
     2281 gttcattttg agacacgtcc ttaatattaa taaattaatt ggcttaaaat tactgattga
     2341 taattataaa gtacctctgt taaaggagta aattctgaac cgattccaaa tcttaattct
     2401 gtaaacgcct ctaaataatc tgggtcacat acatcttctt gtgttaaatc ttgtttcacc
     2461 atttcagtag caagataaat tgctactgat ggtagaaaag catccattgg cataggatat
     2521 ccaccaagtc tatttgacat gccaaaagcc ttagcctttt cacttatcat gtcgtttatt
     2581 ttatcataat tttgagcatc tttttcagat atttttagac ccataacact aggatatgtt
     2641 tgtattggtg atggattgct ttgctctgca actatatact ttaattctaa agccttagtc
     2701 aatataactt catccatata ctcatttaca ccaaatacat ttatatcttt tccatatttt
     2761 ttaacttgtt tttcaatatc ttcatttaaa aactgcttaa ctttgttttt gtcttcttca
     2821 gtatttatat ttggagtatt aacttgtaca aatggcaatc ctatattttt gcaagtctct
     2881 ttaatcattt ctaatctttt ggctatatta acatctttca aatcatctgt agaggcataa
     2941 tgaataaaag cttttgctcc catttctttt gaacgttcag ctaatacttt tcctctttcc
     3001 tctgcactaa cccctagatt cacatcaaca aattggctta attggttttt atcatctccc
     3061 ataggggcag aaatagttat tatttctgga cgcttttcct ttaccttttg taaagcaggt
     3121 aaaagccctg cttggtcagt tgaaactaca atagcctgaa cttctttatc gtcagccaac
     3181 tttactatct tattaatagc actgtctata ttagaagtaa aattctctgg caatacaaca
     3241 tgctttatag tttgtttaac tttagtttga ccatctttgt ctttatcaat tttattctct
     3301 tcctcatatt cttttgccat ctcttccacc atattgtatt gtactttatt ttcagataat
     3361 ggttgagtta ccactgcaac tttaaaatca tctataattt tctctttatc tttcttgcct
     3421 acaagtaaac tacaacctgt aagtccaata attaaacata tggatagcat aaatgctgtt
     3481 atttttctaa gcattaatta aaacctccta aatttgtaca ttcttatcac tattactaat
     3541 tttatcatac ttcaaagaaa aattatatta taaaaaatta tatacatgct attataatcc
     3601 aatctaaaaa gtactatggg tagattaggt ttagattaaa cctacccata gtactaaaaa
     3661 ttaatatatt ataaaattaa attaattaca aattattatg ctacttgttt gttgcttttt
     3721 ataaagttac caactattcc acccaatata gctggtatta cccagttaaa gcccaaacct
     3781 gcaagtggca agctttgtat aaatggaaca gctcctgtca ttccatttat agctgataat
     3841 aaactaatca ctaatgtaac atatgctgca cctttaaaag cattatcatt ctttatatgt
     3901 ttaccagcca actccataac aactattgct aataaaacag gatatatagt ttgtaggatt
     3961 ggaactgata tggctattat agaatcaaca cctaaatttg atataagcat actagctaca
     4021 catataccta taaccagttg tttataactc gccttactta ttccttcaaa ataagttgat
     4081 attgatgctg ttaaacctgt agaagtagtc agacaggcaa gtgccacaat tacacctaaa
     4141 attacctttc ctacttgacc atataacaat atactagtta tttcaactaa taaagatgct
     4201 tgaggtatac tagtatcata taaagttgaa actgttgcac ctaaataagt aagaccacca
     4261 tatactactg ctagaaatac acaggctaca aaaccagctt taatagtcat tcctatttgg
     4321 tctttatctg atgtatatcc tttagctatt atagacatca acattatagt tgccatagat
     4381 gctgcaccta gagcgtccat tgtttgataa ccttgtgata taccttcacc aaatagattt
     4441 ttatctatca tatgctcagg actcataggt cctgctggag aaattatacc ctttactatt
     4501 aaaacaaata atactaataa taacgctgga gttaaatact tacctattat gtctataacc
     4561 ttagaagacc taatagtaaa tgctaaagtt agcccaaaga agattattga aaatacagct
     4621 ttagctactg gaccatggtc tccaaatata ggcataatac ccatttcata tgttgttgcg
     4681 caagttcttg gtattacaag aagtgctaaa catgctagag cagctacact cattactctt
     4741 gctaaccctt taccagctct cctaagtaca tcatccatgc ttcccttaca tcttgctgtt
     4801 gctataataa caagcattgc tataccagca tctgcaacaa caaatccaat aagaggaatt
     4861 aaccagtctt tgccggattc catccctaaa aatggaggaa acataagatt tcctgcacca
     4921 aagaacatag aaaataaagc aaaaccaact ataaaaatat ctctcatctg cttactcaca
     4981 ctaaattacc ccctttgaaa atattttgaa tattaagata atcttacttt attttcagaa
     5041 aaatgtcaat ctggtaaaaa ttcacacatt gttaacgttt tcccaattta ttttatgaat
     5101 atttttttca ttttatgata attcccatca atttcgttaa atcaattgct tgtgacacat
     5161 caaaaattgc tcctctaata tcatctatac caatctcaat tccctcaata ttattagtag
     5221 taaaatctat gccagaaagt ttagttccat taaagtttgc tttttttaaa tttgtatact
     5281 caaatactag attgtcacct tttacttctt gaaatacaga attttcaaaa tttgaatcta
     5341 agatagaaac tatcttaaat ttagagaatg aaaagttaga atacttacct aaaacatcct
     5401 tgaacaaaac gtttttaagt atacaatcat caaatctaac tcctatcaat ttactatttt
     5461 taaattcaac tctgtatata cttcctccat caaaaaatgt atttgataaa ttgcagtttt
     5521 caaataccac atcaagtaaa tcaatctttt ttaaattaca attttcaaaa gacacatttc
     5581 taaatataca agattcaaag ctaactctta cagaatctaa atcacttata acttcatttt
     5641 ctataatttt attaaatatt ttttcatcat taaatatatc ttcaagtata tcttcaagat
     5701 tttcaataat ttctaattct cctgtaaatt taggtttttg cactttaata attttagtca
     5761 ttttcccctc cactataaat tatattattt ttgatattat tttttttaat atattaactt
     5821 aatatacata tttgatatat taaaaagttt ttacgaaata tccatgtatt cagtactttt
     5881 ataattatat cacaaactca tttataataa acttccatta atagtataaa acaagttaga
     5941 ataattgaca ttatactcat acatattcaa ttattggaaa aagtaatttt tttcaataaa
     6001 taaccttaat tttttataaa atcgacaaaa aaacgaataa ttttattata tttattgtaa
     6061 ataatacaat attacgatat aatacaacag aaaaacaaat agaaaatgtt catataatat
     6121 tagtaatacg gtctaatagt ttctacaaac tcaccttaaa tgggttttct atgaatagaa
     6181 tgtcaactct gaatatatgt tctattctat ctatatgaat atatattttt ttgtttatat
     6241 cttaaagttt tttggaggta aaaatggtac caaacactaa caatgtacaa ccaggtggaa
     6301 ataattcatc attaagcttt ttagacaaaa tgtttaaatt tagcgaacgt ggctctagcc
     6361 ttaaaacaga agtaataggt ggagttacta cattcttaac aatggcatac atagttttcg
     6421 taaatccagc aatattatct gaagctggaa tggataaagc cgcccttata acagttacca
     6481 tactagctac agccattggt acacttatat ttgcgatttt aggaaatgca ccatttgctc
     6541 tagctcctgg tatgggatta aatgcatttt tcacatactc tttggttata ggtgcaggaa
     6601 tcccatggca aacaggtctt ggggtagttt ttctatctgg atgtgtcttc tttacactcg
     6661 cagtaacagg tcttcgaaag acaattgcag acgcaattcc tagagaattg gcagtagcat
     6721 cttcagcagg tataggtctt ttccttgctt ttgttggttt aaaaaatatg ggtgttataa
     6781 tagctaatcc agatactata gtttcacttg cagattttac accaacagtt ttaatagccc
     6841 tttttgctct tatcttaatg gcaatactag aatataaaaa agttaaaggt ggaatattga
     6901 taagtatagt agttgcaaca ataatatctg ttatatttgg atttgtggag ttgccaacag
     6961 caataatctc aacacctcct agtatagcac cagttgcttt taaattagat attgtaggtg
     7021 cattgaaatt ctcattaatt ggacctatat tctctttcat gtttatagac atgtttgatt
     7081 cattagcatt tcttattact tgttgtaaac aaatgggtct tgaagatgag aatggtgaaa
     7141 taaaagattt aggaagaatg ttatatgcag atgtttcttc tactttaata ggctcattct
     7201 taggaactag tactgttaca acatttggag aatctgcttc tggtattgca gcaggtgcaa
     7261 gaacaggact tgcttcaata gtaacagcag cattattctt agctacactt atatttacac
     7321 cattacttgg tatagttcct acttacgctg cagctcctgc acttgtaatg gttggtgtgt
     7381 ttatgtttga aagcataaaa cagattgatt ttgctgatag caaaatagct gtacctgctt
     7441 ttgttacagt tttattgatg cctttaactt atagtataag tattggttta tgctttggat
     7501 ttatttcata tataataatg catgtagtag ctaaagaaac tgataaaatc agtattacac
     7561 tttggattat aggtttactt gcagttgtaa acttagttct aagttaatat ttttaaatag
     7621 ttctaggtca atattttaaa ttatatagaa taaaatctta tttattttaa atttataaat
     7681 agcaaaatca ctttatatgt aaattgattt tgctattaac tttgtataga aaatttatta
     7741 tcataatata tagatataaa aatatataga aaaggagtgg tgtttatgtc acaattactt
     7801 ataaaaaatg catatctaat tactatgaat gcagatagag aggtatatga aaatggaagc
     7861 atacttattg aaaataatat tataaaagag gttggtaagt ttgatattaa tattgtagat
     7921 aaagatgcag agatatatga tgctaaagga aaaatactaa tgccagggct agtcaatact
     7981 catgttcact tatctcaaca attaggacgt ggtcttgcag atgatgttgt acttttaact
     8041 tggcttagag aaagaatttg gccatacgaa agtagcttca attatgaaga ttccttgatt
     8101 tcatctactg catgttgtgt tgaattgata aaatctggtg tcactacatt tttagaatct
     8161 ggtggtcaat atgtagatgc tatggttgaa gctgttgata aaactggact tagagcctgt
     8221 ttagctaaat cagttatgga tacaggagat ggtcttcctg aggcatggca aaaaactact
     8281 gatgaggaaa tcaacactca attagactta ttccataagt ttaataatac atctaatgag
     8341 agaattaaaa tatggtttgc attaagaact atttttaata caactgaaga attacttgtt
     8401 agaactaaag aactagctga taaatacaat acaggtatac atatgcatat tgctgaaata
     8461 gctgatgaaa tttcatttgt aaagcaaaac aatggagttg gaacagttga ataccttgat
     8521 aaactaggtg tattaggacc aaatctttta gctgctcata cagtttggct tactcataag
     8581 gaaattgatt tatttagatt acatgatgta aaagtttctc atgacccagg ttcagctatg
     8641 aaagtggttc tagggtttgc atcaattcct gaaatgttag aaaaaggaat acctgtttca
     8701 attggtactg atggagctcc ttcaaataat agaatggacc ttatgaggga catgtatctt
     8761 acatcactaa tacataaagg aagaacttta aacccaactg tcgtaccagc tgaagaagtc
     8821 ttagaaatgg ctactataaa tggagcaaaa tgtgctttac tagaaaaaga aattggtagt
     8881 cttgaagtag gaaaaaaagc tgatatgatt attctaaatc cagatacaat tcattgttta
     8941 ccaatgcata atccaattgg aaatatagtt tattctatga ctagtgaaaa tgtagactct
     9001 actatttgtg atggtaaatg gttgatgaag gaaagaaaag tattagttgt agatgaatct
     9061 gaattattaa ataaagttaa aaaacaagct tctaaaataa aagataaagc agaagttaaa
     9121 attccatcta aatttaaaat tataaaataa cataaatttc aattgttttg taaatatgaa
     9181 tatataaata tatttatata catcatatat acaaaacaat tgttttttta ttttatattc
     9241 attagcattg ctcataaaat acaccataat tccagaaaaa ataaccaatc agttaggatt
     9301 acatcatcta agcaatatat aaacgtctta gtttataagt tttattctac tcttatctaa
     9361 aaccatcttt aaagagtttt caactgtttc aattacatct ttataactca agtacacatt
     9421 tgcgtagata caatcttcat ataaattata ttcagttact ctacaataca gcagttttcc
     9481 tccatctaat aaattgctca aaatatagtt ttcttttctt gggacatagc ctatcacata
     9541 tccagtttca ccaactattt taatagcttt ttcatcatat agattatctt tctctctagt
     9601 taaatagtat atcttacttt tctccaattc tttttcaaga tattctctat ttctaaacct
     9661 tgaaccagca acagctattg aagtcaaata gatatcctta ccatgttcat ctacttttat
     9721 tttttcaata tttacaaatt ctttttttaa attattactt tcatataata ctcttcttaa
     9781 ttcatccttc aatataacat ttttttcttt ttcatacgct tctttaacaa tttttaattc
     9841 ttcaagagat agtttctctc tgtatctttt taagatattt atggattctt ttcttacttc
     9901 atttatattt ccaaatattc cttttaaaga tatattcttt ccttcaggat atagtgattt
     9961 acttccttta agcacaataa aaaataacat atcatcataa tattcttgag tcaatttatc
    10021 tttttctata tctttcattt ttccaattaa atcatctatt ttaaacgtct cttcaaaaaa
    10081 atttaataat tttaatctag aacttgtcgt tcccttttta gatatatgcc aatatacctc
    10141 aaaatctcta atatctctat ttagatatgg caacaagtca tcaaaagata agtttacatc
    10201 ataaatttct gacattttta taatatcttc tgtttttcca ctagcatctc ttaatgcttc
    10261 aaaatatata tttttccatt tttcactgag caagatacca tttatttctt tttcaaattc
    10321 aatcttattg cactctatat tttcatcttt taagacgttt atagctatta ggaaaattga
    10381 atataaactt tcaaagttag tcccataatt tactacagta ggtaaaaatc gattcactaa
    10441 ttctaattta ttacctatat atttaaattc tacacttagc aagtaattgc atataagacg
    10501 tgctaaatta tttatctttt ctttatctaa atctcgtttt tcaagatatt cattcaaatc
    10561 tacaattgaa ataatatagt tcatcaataa tcgttcgtac tttgtatcct tataaccatc
    10621 ttcaattaga taggaattta ttttagaatc taaattttcc atattaacaa tagcaaatac
    10681 ttttattgaa cctgtagcct ttttagataa attaaataaa tatgtattgt aaaattcaag
    10741 ttttttaata gtattactca gataaaatac gtattctcca gatttagaaa atgtatctac
    10801 aacttctctt aagttttcta catttagata tttctcactt aaaactaacc ctagttttac
    10861 atactcaact tttttactct tatatattaa ttctttagtt aatttatata accatttctc
    10921 atcaataatc atttctttta ccaaagtatt tatctttttc ataaaaattt cgaaataaca
    10981 aattaaagac ttagaattac taccctccaa taaataaatt tcattagatt cacttttata
    11041 catatttaaa atttcatcta ctagattatt tgcaagctct acttgttctc ttaacggtat
    11101 actttcatat atacataggt aattcatttc aaatgattct ttgtatccat cataaccatt
    11161 aaattcctgc aatcgcttaa aaacattctc caccaaatta tcctccttta catgatgtaa
    11221 tttatattga ttatactact tatttcatca agtttacatt tatacgcaaa aaaatattac
    11281 tagaaattga aattctagta atatccttat tattaattat gtaaataccc tatcttataa
    11341 ctgtaaataa tatccttatt aattttaatt acttttaata aaaatctatc ctagatttag
    11401 acttctctat gcatatatta tactaatatt agacatcata atcatacact ttttcttttt
    11461 catatctcat gtttacaaac aatgcaagta aaattccaac aaatgttatt gccacattaa
    11521 acaatacaac atattttggt ccattaactc cccctgattt tgtaataata cctgctatat
    11581 ttaaaataac atagtttgct atactagatg caatcataac tatagatgtt atttttcctt
    11641 tattagttgg aaacatttca tttgcagttg acgttgttaa cttaagaaca cctcctgctg
    11701 ctgaataacc tattacaaat ccccctaaca tacaaatagt tggagtttga acaaaatata
    11761 caactaatag cataagtaaa gctattatag gatatataac taatattctt attggtttaa
    11821 tatatttttt aataagtaaa gaagttatta atactgcaca tattactccc attgagtaaa
    11881 aggattgaat ttttgtaggg tcagccatcc catacaattt acccaattcc tgattacaat
    11941 ttaaccataa tacaaatgta gatgtacatg taaatccaat acaaacaaga gctatacttg
    12001 ttggtgtaaa tttcatcttc tctgatttaa ctgttttgcc tttgttatca ataacattat
    12061 tagctggtgg aaatggtaac acagctatca aaaaggcatc tactacaata agaatcgctg
    12121 ttactataaa aataacttta aatgatattg aatttgctgc aacaaatcca attataaaag
    12181 gaagcaaaaa ttgtgctaaa gctattgtaa atttagtaaa catatttgct attgttccta
    12241 aactagcaaa tatctccata caagaaggag ttacacaggt atctaagaat gaatttgctg
    12301 cccctccaat tattgcaaat acatatgcta tataaaaatt tggtgaaaat acaattccaa
    12361 caaagaaaat tgcatgtaga aagtttccta taagaccact tacttttctt ccatacttat
    12421 ctgaaatagg tcctgcaaat ggataagata taagtctccc taatcctagt gcagcaataa
    12481 cagctaaaac aatacttaca tccaatgttc catctgataa tgttttagcc ccccacattg
    12541 atgaaaattc tggtttgtat tgtcctaaaa ttgaaattcc aataccaagt aaaaaatatg
    12601 aaaaatacaa cgctatggca gtaggataat attttttatt gttcataatt tttctcccat
    12661 tcttgttaat ttattaacat ttttgtgtac aataaactca tccccataat ggatttaaag
    12721 tatagtcagt taaagcttca aatttaactg gactatgagg caaaacccac tatgacaatt
    12781 tgtaatttat tattaaaaat attgaaatac gtatgttttt tcattgttta agatattact
    12841 cttatctatt taaagcataa ttcattaact tcttctacag gcatttctaa tccagtatat
    12901 agattaaaag cttctgctcc ttggtataat aacattccta atccaccaat agccttacat
    12961 ccattagctt cagcatcttc aatcattttt gttttcttag gattataaac gacatctgtt
    13021 actactaaat cttttcttaa aacactagta tcttttatat ttgtttcatt gtcataaggc
    13081 ttcataccaa taagagttgc atttgtcaat atatcacttg attgtatctc ctcataaagt
    13141 ttgtttgtat cttctaaatc atatagatta acaacacatt ctggtacatc tttttttata
    13201 ttctcaactg tttgctctgc tcttttataa aaatcatcct taggattaaa gatagatatt
    13261 tctcttgctc catctaaagc acattgtact tgtatagcag ttgctgcacc accagcaccc
    13321 ataattgtaa tctttttacc tttaacttct accccatttt cttttaaatt acgaacatag
    13381 cctactccat ctgttatatg accaactaac tttccattgt catttacaat tgtattacat
    13441 gctccaatta tacgagctgc tggagaaagt tcatccatat attctgtaac agcagattta
    13501 catggcatag taacattggc tccttttata ttaaaagttt ttattgctaa aagtgcctct
    13561 tttaccttat ctactgtgat atcaaaggct aaatatctat aatctaaatc taatttctta
    13621 aaactataat tatacatcac tggtgattta gagtgaccaa ccggtgtacc tattaatgca
    13681 aataatcctg ttctacctga aatttccata actataatct cctctcaata ttatcatttt
    13741 ctttgcaaat aacctattca gttatttaca aagaaaaatt tatttattat ttatatattt
    13801 aatgctactt caaaagcttc ttttgttgca tctaatgccc tacgagcttt tactgcatca
    13861 ccaacaatat aagtttcttt tacaacttct tttataactt cacttaatgg gtcgtaatta
    13921 cgagaaccca tagcaagtac tactgaatca aaacctttta aatcatgctc tgtgccatct
    13981 gtaagtaaat agtcgacacc atcatcataa aatttagaaa cttttgcacc agtaatggta
    14041 tgtatattat aattttcaaa atcattcata aggaatttac ggtgttctga aattacatct
    14101 gcaccaactt cttctctaag ctcaataaca gtaacctcat gttcttgttc tccaaggaag
    14161 gctgccactt cacatccaac cattccacct ccaacaacaa gtactttttt cccacaagat
    14221 tttttaccat caagaaggtc tacagcatga attaatcctg aatcattgat tcctggtata
    14281 ggaagtacaa gtggagttgc tccagtagct ataataacta catcaggtga tttctccttt
    14341 ataagttcta ctgtagcttc tgtattagta catattttta caccatattg attacatttt
    14401 gaaatataac ttctaactaa gttagtaata tctcctttcc ctggaggata tgcagctaaa
    14461 cgcatttgcc caccaagaac ttcagatttt tcaactaaag ttacattatg tcctctttta
    14521 gcacataccc atgctgcaag catacctcca acacctccac caacaacaag aacttctttg
    14581 ctaatttcag ctggcttaag ctctgcttca tgtcctaata taggatttgc aagacaggct
    14641 ataggtttgc cttggaacat atttggtaca catccttgta agcatgcaat acatggtatc
    14701 atttcgtcta gttttccatt tttagcttta ttaggcattt ctgggtcagc caagctttgt
    14761 ctaccgaatg ctactaaatc acatcttcct tgtctgacca ttaactcagc aaactgtggc
    14821 tctgtataac gtccaactgt tattactggt atactaacag ctttttttat ttcagtaact
    14881 aattctgcac tgaatccagc atgtaaagtc gtaggagccc acatatattc atcatgtata
    14941 tgtatactac gtgaaacatg aagtccatct attccacaat cttctagata tgctgctatt
    15001 actgaactat cgtgtacatc aattcctcct ggaacatcat cagtgctgtt tatacgacat
    15061 aatattgcta gagaatttcc tactttttta cgtatatttt caatgataag acgaggtaat
    15121 ctcattctat tttcaaaaca tccaccaaac tcatctacac gcttatttgt tctttgtgat
    15181 ataaaactac taaccagata tccatgagcg cagtgaactt caacagcatc tgcaccagct
    15241 ttttgtgcac gtaatgcagc atctccataa gattctataa gttcatatat ttcttctgtt
    15301 gttattgcta gtggtgtatt acgaccattt gatgcaggta ttgcagaagc agcttttaat
    15361 ggatgtcctg ctactttttc atttccttca gaaccagcat gttgtaattg tatagatacc
    15421 ttcgaacctg catcatgaca agcatctatt acatttttaa aactatctat tgtgctatca
    15481 tcatataaac aaggcttacg aggacctcct tttgcttttt tgtctacaac actagattct
    15541 atagttatta atccaaaacc acctaaagca cgctcaagat aataagcctt agaagtttca
    15601 cttaaagtac catctgtgtt tgcaaaattg tttcccatag ggggaactac aaaacgattt
    15661 ggaactgtca ttgaaccaat tcttataggt gtaaacatag aactaatctt cataaggttt
    15721 tccttccttt caagttttat attataatac tctgtatatt tttatattat ataagtttat
    15781 tttggagata cttcagacca tgctttatcc aaaacttttt tagttgcatt aaatacagtt
    15841 tcagcagcag tggataaccc tttagatatt agagaatcag atattacttc tgcccctatg
    15901 actcttggtg atatattgtg ttcttttatt attttcacaa attcctctgt gtttccatat
    15961 ccttcacctg gagccaaacg gtcatgtagt gattcatccc taagtttttc ataaggagtt
    16021 tctaaaacat cacacacttg aattgatact atcttatctg ctggaacagg tgcaaggtcc
    16081 tctgctgtct gctttgctct agcccagtgc caagtatcta atataagcat tgcattttca
    16141 cgattacatc ctttcacaac acgccatgca gtagccaaat ctggtacacc actatatggc
    16201 ataaactcaa gacctataat aagttcacct gctctgtcac ataattctcc caatgcagtt
    16261 ataatttgtt cttctggtat catctctaat aatccacaat ttatatgttt aacattaaat
    16321 aagtgagcca tatgataaac attttgctct ttttctcttt gagcttccgt tctatcttct
    16381 tctcttcccc attgagttat atattctact tctgtaactt tcatatcata ttcattaaga
    16441 attgcaatca tttcttcatc tgtaacacct gcattttttg catcaatata attttcagca
    16501 cgcaatccaa taccatcaaa cccagcttct tttgcaatac gaactctatc acggaatgaa
    16561 acttctgttc ccaatgtata agaactaatt gtaattggaa tattaagatt tttttgatta
    16621 ttcatagctt aaatttgccc cctttatttt ctttataatt aatagtattt ttgattttat
    16681 atttttattt atcctactag tagtattatt ttaccttaaa aataaaagta ttttttgatt
    16741 tctatttatc tagattttat aaatttattg tatcacgaga ctttactaat aacaaattga
    16801 taatttccta ttaattcata gattttgttt atagatttta tttataagat aattcttgta
    16861 tttttctatt aggtcgtata aatatattta tcttataatt cagacaaaaa acgataaatt
    16921 tttttaaaag cttttattct tactctataa tcatatataa taaaaatgta aaaatatctt
    16981 aaaggttaac ttctacaaat atataaataa aaatacctat gaaaggatga acaaaaattg
    17041 aatttatatc acttacgcta ttttgttaca ttggcacatt tagaacacta tacaaaagcg
    17101 gcagaaaatt tatctataac acaaccaagc ttaagtcatg ctatttcttt actagaaaat
    17161 gaacttgggg tggctctatt tgaaaaagaa ggtagaaata tagtccttac taagtatgga
    17221 aaaatatttt taaaagatgt ggaaaaatca ttagaaattt tagattcaag tgtaaaatca
    17281 ttaaaaataa ctggtactgg tgaaggacaa atagacttag cttttcttag aacacttggt
    17341 actgatttta tcccagacat agtgcacaaa tttttaaaat ctaatcctgc taaatcaata
    17401 gattttaaat ttcatactgg tgtaactact gacattattc aaggtcttaa agaaagaaaa
    17461 tatgatattg cattttgttc gaaacttgaa aaagaaaagg gaattgaatt tatacctgtt
    17521 gccaaacaag atttagtact aattgtacca tatagtcatc cactagctgc aaaggatact
    17581 atagatttaa aagaaaccat accttatcct caaatagtat ttaatcagag aagtggtctt
    17641 agatatataa ttgatgatat gtttaaaaaa ataaatcaac agcctaatat agtctatgaa
    17701 gtagaggaag accaagtaat tgctggatta gttgcaaaaa atttcggaat tgctgtagtc
    17761 ccaaatatga atatgcttag ctttacaaaa gtaaaagtaa ttcagattat tcacccaagt
    17821 tgggaaagaa acttttactt agcatttata aaagatagat atttgcctcc tgcaattaaa
    17881 aactttaaaa actttgtcat taaaaacgct caattataat ttattaataa aggggttatt
    17941 tttaaatatc ctcttttatt ctttttataa aataaaaaaa tctatacttt taaaagtata
    18001 gattcctaac tgacagtcgg catccgagtc tcagtaatta tcgttataca acagcaactg
    18061 gattcagttc ctgaataact aggttactta cattatatca gattttttaa aatttgtcaa
    18121 ttgtatctaa cgcatttttt caagccatgg tccaacaggc tctacaatag gaacttcttt
    18181 aaatccccaa tttcttatat cattcacact cgttggagta tatattttgt ttctgacaga
    18241 tttgaatatt ttgttttttt ctccatattg catacacttt ggattagctt ctattaaccc
    18301 tcttcttgct ctgctaagag gattacataa aatatctgct atttctaaac cttctctata
    18361 gtcagcatct tcttttttgg caattataaa acccttaacc ttatctctta attcttcatc
    18421 tatatctaag tatattgttc cattattaaa tacatcaaaa aacgcctttt gtacttttaa
    18481 gtttgagaca tcatcccttg actctataac tattcgtgca gattctacag tttcattatt
    18541 tacaaatgaa taaaaattct gaagaacatg tataaaagcc acaacatacg gttcctttga
    18601 aggtgcataa tatttcttta atctagcttt atctactgtt actgatatta tagtacactc
    18661 taatctagat agaaataaag gcaactcatc atagaataac ttaatctgtg aatcatcaaa
    18721 cttctcaaat gctcttgatt ttcttgatat atctgtcaaa tgtattccta tgttactggt
    18781 tccaaaacaa tactctttaa atgcatccat ttctttcctc aaagaacata cataatcgcc
    18841 ttgacttact gcatatttat attcaaaaat tactcctgtc aatgtaaatg ggtcttcatg
    18901 atttatatca gctcttccac tttcatcaat aaacattata tattttttct ttataatctt
    18961 actcaccctc ttaacatatg atgatagcat ctatattcat tttatatgag ttttaaaaat
    19021 attccaagat ttttatataa cttaaactta attttatact aaaaaattga gatgtaaaac
    19081 aatcaagctt ttattgattg aatttcacat ctcaattata attatttttc aatatatatt
    19141 tttactattt tgcaagaata tccattaatt tatccattac tatataagat actccgcctc
    19201 ctatttgata aggagtttca acttttttat ttgcaaatac tctttgttgc tctgatgata
    19261 atcctgaatt atccattaaa actattggtg aatgtaattt tacagctaat ggtccagctg
    19321 ataaagcatc tactaaagca tctgatttac taactaatac agcctgtaaa tcagtatctc
    19381 catagaattt ttctatcact ttggcattag tatctactct actatctcca tatattctat
    19441 ttcctgaaat atcttcagta gttatatcat tcatttttgc tattactgaa tcgttaattg
    19501 cacttggtcc acctataaag tatgcatttt ctaattttct ttctgttagc catttataag
    19561 attcttctgt aattccatct ttattgacta atattatagg ttgtttttct tccccagctt
    19621 tagaagctac agataaagca tctgcctccc catttccaga aaccatatat gcagttttta
    19681 tttcttctcg atttccaagt tcttcagcta ttcttgtagc agtttgatat ctatcttgac
    19741 ctgctattct ctttgtttct gtgttgaaag tagtttttat ttcttgctct aatttagaac
    19801 ttattgcatt ttctccacct atcaagatta ctgttttagg tgctaataac ttcatttctt
    19861 caacagtttc agttggtaaa ttagctgtat tagtaagtag tattgttgag tcatagctac
    19921 ttgctaaagg agttgctgtt ataccatcag ctattgcatt accatttact aatattacag
    19981 tatctgaact atcagcattc cagtttccaa ctgaacttat ctttttagct gtatcaaatc
    20041 tatttgcacc tataattgta tcccatgtag gatttatagt tccatcttca ttaaaagtat
    20101 aatctttccc atctattttt tgagaaccag ttagcatctt tccatctttg tctaaatagt
    20161 aagacttacc atctatttgt tgccatcctg tctgcattat tcctgttttg tcaaagtaat
    20221 acttattacc atctatttct tgccatcctg ttactgctaa accatcagat cctagatagt
    20281 atttctttcc attcttttct tgccatcctg tttgcattac tccatctgag ttaaagtaat
    20341 aagtttttcc gtctattact ttccatccag atactcttgc tccatcagtt cctaaataat
    20401 attttttgtt attaattgtt ttccatcctg tttgcattac tcctgaagca tcaaagtaat
    20461 acatttttcc atttactttt agccaactat tcttttggta tgttccatca gattttacat
    20521 aatacttctt accattttct gttttccatc ctgtttgatt tagatttatc ttatattttg
    20581 ccgctatctt tacatagctg tcgttactat aatctataat tacatctgaa cctacaggaa
    20641 cttggtcaaa taaccatcta acatctttat tgtgcattct tatacatcca ccagaaatat
    20701 gctttcctat agaactctca ttattatttc catgtattcc atatgtatct ccataagttc
    20761 cttttaatgc taatcccatc catctatctc ctaatggatt tcttggactt cctccaggta
    20821 tattaccttt ataatatggt ctatttttta tcttgttaac tacttttgtt ttccctgttg
    20881 gtgtttctga ccccttttta cctgttgcaa ctctaaattc ttttacaagt ttattattta
    20941 caaaatatcc tagagtattt tttcttgaat ttactataat cacatgttta ctggctgcgc
    21001 ttgattctgg tacgccacat gcaaatacta aaactaaagt cagtgctagt actaaatact
    21061 tttttagact ttttttcact ataacttccc cctcttttag aaacattact acatgtgtct
    21121 aattataaca taaaattacc aataccttat atttacaaat ttgttacatt tatacatata
    21181 agtatgtatt ctactcttca tacccattag gattcatact ttgccatctc catgaatctt
    21241 cacacatctc ttcaagttca tacttagctt tccatccaag ctctttttct gcctttgaag
    21301 aatctgcata acacatggct acatctccag cccttcttcc tacaatctta tatggtattt
    21361 cttttccact tgctttagaa aatgctttta ctaaatcaag aacactatac ccctttccag
    21421 ttcctaaatt ataaacaaca agtcctggat tttcttctaa tttttgtaat gcttttacat
    21481 gaccagcagc taaatctaat acatgtatat agtctctaac tccagttcca tcatgtgttg
    21541 gatagtcatt accataaaca ctcaattcct tcaatttacc tactgctatt tttgttatat
    21601 aaggcattag attgctcgga actccatttg gttcttcacc tattctacca cttttatgag
    21661 caccgactgg attaaagtat cttaaaagag ctatatccaa tgacttatct gcttttgata
    21721 tatctactaa catctgctct atcataagtt tagttcttcc atatggattg gttacagaaa
    21781 gtggaaaatc ctctaatata ggacaagtat gtgggtctcc atatacagta gctgaagaac
    21841 taaatacaaa ctttttaaca ccatattctc tcattaattc aaataaattt aaggtactta
    21901 ttagattatt gctgtaatat tcaattggtt tttctactga ttcaccaact gcttttaaag
    21961 ctgcaaagtg tataattgat tcaatattat tttctttaaa tacaacatgc atttcatctt
    22021 tatttctgat atcaatatta taaaatttta ctggtttttt agataattcc ttgattctat
    22081 caaccactat tgagttgcta ttacttaagt tatcaactat aacaacttca taacctgatt
    22141 ctaaaagttc tattgctgta tggcttccta tatatcctgc tcctccagct actaaaactg
    22201 ccattgtaat tacctctttt ctaatattta tttttcactt actttttttc tcatatagtc
    22261 cattaaatca tcttttaaat tttctctctt tagagcaaag tctattgttg cctctaaaaa
    22321 accaatttta tctcccacat catatcttct accttcaaag ttataagcat aaatagcctc
    22381 ttgttttcca agagtttgaa gtgcatctgt cagttgtatc tctccacctt taccaggtgt
    22441 ctgtttctct aatatgctaa atattgcagg agttattata tatcttccaa gtatagctat
    22501 atcagatgga gcttcttcaa ttgcaggttt ttcaaccata tctttaactt tatatactct
    22561 atcttcgata tgttttacat ccaatatacc atatttatta gtatcttctt tagctatctt
    22621 ttgaacacct aatacagttg ttttatactc attataagta tctattaatt gttttaagca
    22681 cggaacatct gaatctacta tatcatcacc taataatacg gcaaaaggtt catcacctat
    22741 aaaacttttt gcacagtaaa ttgcatgtcc aagacctttt ggctcttttt gtcttatata
    22801 atgaatattt accatatttg atatgtctct taccatttct aacatctctg tcttaccttt
    22861 ttgctctagt tctaactcta attcaactga tttatcaaaa tgatcttcta tagatttttt
    22921 atttctacct gttattataa gaatttcttc aattcccgat tctatagctt cttctattat
    22981 atactgtaaa gttggtttat ctactatagg aagcatttct ttgggctgtg attttgttgc
    23041 tggtaaaaat ctagttccta gccctgctgc tggtattatt gcttttttaa ctgtcatctg
    23101 cattttttca cccttcttcg taaattttct attgtaccaa attaatatac catatttata
    23161 ctttttcttc aaactctata ttatcccaat atttaatgct ataagataat ataaggtaga
    23221 tgaaatttca tctaccttta gttttataag ttaatattgt attgtttata ttttatccaa
    23281 aacttataca ggtactaaat ttcatctaag atgcctttta tatcactatc tatatcttca
    23341 tctagataag aatctaaatc ttctaagtca acttctagtt cttgatatgt catatcaaca
    23401 ctgacatcct catctatatc catttctgat attaattctc ctaaaatact atttccatca
    23461 gaatctaaac cactatctat atcttcatct atatagtttt catttaaaat ttcatctaaa
    23521 tcaatctcgt aataaacttc attatttttt tcatcccctg agtccaatcc taagaactca
    23581 tagttaaaag tccaaacatt gtatttactt gacatacttt attcctcctg tgaaaaacat
    23641 attttcatct agtttattgg ttccataatc aacagagtta tatatttttt caattatgtc
    23701 ttttcttctt tcttttgtac tattatttaa tgtcaattgt ttaaatacat atgtatttgt
    23761 tattctctcg aataatattt cttttatact atcatcatca atatttctat agtttttcca
    23821 caataactca aataacttaa acatttccct actagtttca tctacataat aaggatacat
    23881 attttctact aaatggttta atcttgatat ggcttgtttt aaaaatacat caagacttgc
    23941 actttcactt tcttttaaca ttctataatt gtatttcttc ttatcttctc ctgaaattac
    24001 aaagatgata ttgtctaaac ttttttcaac ttcatttaaa taactgctta tattatatga
    24061 taaatcagac aatagatttt taatctgctt aaacacttgt acatcaagtt tagtattata
    24121 cttttcaaac aaatctattt cttgattttc taatattctt ggcatatatg ctgacaataa
    24181 atctaaattg tttctttgtt cttgtatttg cttataaagt gaatttataa cttctattct
    24241 tatttcatca tttaactctt tttctaaaat cttatcaaat tcagatttca tatagtaaat
    24301 atttccaaag aaattaaata ttatcatatc attcatagct ttagaccaat tcttatatat
    24361 cacttcaata gaattttctt taagtagagg ttgctttgca tatataaata catcttgcat
    24421 aagtttttga accatacttt tacttaagtt taaaagtact ttttgattat taaattttat
    24481 ttccgtttct acaaaattag aattttcgtt ttcaagttct ttattaatta caatgtcttt
    24541 tatatcacta ttttttatag atatgatttc tttttgaaaa gactttagaa tagtcaagct
    24601 atcgaaatct tcttgaataa aaaatggagc atgttttaat gtatatccca ctatttcatt
    24661 ttctatatct tttaggtcat ctatttgcat aatttctcta tttttatcat ttatatgtag
    24721 gttaaataca tttttattat catataatat tatatcttct cctaaattta acttagaaca
    24781 attatctttc gctgacttta caatagtctt cttagatttc ttatttataa aaattaattg
    24841 gtcttgatat tttactaaaa gcatgtttgt gtttttataa tcaaatccat ctatatttcc
    24901 tgatatcttg cagaatacta tttcacaatc tttaggaaga tttcctattt tttggttatc
    24961 tttaactctc tcatttaaat cagatgggac tacactatct atattgttat taaatataga
    25021 tacagacctt actataatcc cctctctttc catatgaaag tatcccttca atgacactct
    25081 tttatcttct aaaatgtatc tttctatatc atcaaaattt atttcaaatc tatttttccc
    25141 actgataaat ataaacttct cattatcttt attaattcca cccatataaa acttattgtc
    25201 atctaaactt aatatagtca tattatcctt tagatagaat ataaattctc tttgactttt
    25261 gcagattaaa tcatattcat ccaaagttat tttcatagtc aaatttgctg taacattttt
    25321 acttgaattt tcacttttta tgtaaaaagg caagttattt ccatagacaa ttatttgaga
    25381 atcttctaaa actgcctcta atttttcttt attgtctgaa aaactatata tatattttac
    25441 acctgtatcc tcattaactt catctgattt gctgattatt atattcccta gatttgttat
    25501 atgaagcttt ttatattctt ttgagtatct acttatgcca ctaagctcaa acccatctga
    25561 aagctcatat atgatttttt ctcctaattt caacatacct tcaccaccta aacagaatta
    25621 taagtatttt ataaaggtga ataaaatgtc tcaaaacaaa taatattttt tttaatactt
    25681 tttattctga gacatagttt ctcctttttt ataatgctct aagcaccttc aactatttct
    25741 tcataaagtt tatctaaatg tttaacatat tcacgatatc cagatttgtt ctttaactca
    25801 cttcttaaat cttcatattt ttgtttaagt tcttcagcac tcaaagaagg agctgattct
    25861 tttaattctt ctaacttata atcatagtac ttatcataca tagctatata tgtatcaata
    25921 gacttcttag ctagttcatt acatttttct tgtgcttctc catattgaga ataatcacct
    25981 atttttattt tatagtatcc attttcttga attattgttg aagaaatacc tttagcttca
    26041 tattcttttg ctgtttcttt tgctgttttt tcaactttaa gtgaacctgc ataaacagtg
    26101 taatatacac ctttaccatc tttatatgct tccatttctt ttgctttctt ttcagcttgt
    26161 gctttttctt gagcctcttt ttcagctttt gccttttctt ctgcttcttt tttctctttt
    26221 tcagcttgtg cttttttctc ttcttcagtt aatttattgt tagcttgttg ttgagttgat
    26281 gactttggaa tattctcttt atcatttttc ttattgttgc ctactgttat aaaatatcct
    26341 acaccaacaa taaccactat aactactgct ataatagttt cttttaatct actcttctta
    26401 gaaacaacct cttcatcatc taaagaatct tctttatctg atacataatt tttcatttct
    26461 ttcttgtttt ctctatattt gtccttagaa gaatttttag attttttatt actattttct
    26521 ttctttttaa taggtattac tactgatttt tcattttcta atttttcttc ttttatttct
    26581 tttgcttttt tatcttctaa gtttttactt tctttttcaa gcttcgattc attatttgaa
    26641 gatacttttt catctaagtt attatttatt ggtttttcta tttttacact acgttcttct
    26701 tctatatgtt gacttggttc ttcttgtttt atttctttca attcattttg ctctatttct
    26761 tctgatttca cttgatgatt ttcaactggt ttatctaaaa ttatatctgg agaaatatca
    26821 tttaaattct cttcttcttg ttcagcttct tcattgtctt ttttaataaa tccaaataat
    26881 ttagccttct ttttactctt tttattttct ggttcaatta tatcttcttt attgctgtcg
    26941 tactctttat ctgtagtatc tttatcttct acaacgtcac ctatatcttg attattacta
    27001 tctacttctt ctttagtccc ttcttcagca tcatcttcta ttgaaatact acttctaaag
    27061 caaatatctt tatctgcatt ttttatcatg taaatgtatt cttcaatatc gatatcttta
    27121 tttcttctag ttctttttga tgcaatttca tgattttcta attcactata gttatctttt
    27181 attagattct gtgctttatt ccatgtatct tcactaattt ctgttttatt tttaattaca
    27241 tagtccacaa attccttaaa attttcagga ccaaattctc caattatagt cagtctcatt
    27301 gcaccttcta taaggtctct atttcttctc tttaaaaatt ctaatacaac tgttgcacaa
    27361 gcaaaatctt taataatttc tttgccaaaa ccatatgctt caacagaatg aagttcttct
    27421 aatccacata acttcttata ttcattgtgt attgtttgag atatactttc attatttaag
    27481 ctatttaact tatctataaa cttcgattct atatcatttg attttgattc atgttttact
    27541 tttgaatttt taatttcatt tagtagcatg tcattttctg aattatcttt acctaattgc
    27601 aaatcctcaa tttcagttgc ttttatttct actttgtttt gttcgttatt ctcttccaca
    27661 ttattaggta ccacatcttt attactagct aatattttgt ttacacagaa tataatttct
    27721 ctttttaatt ctcttttcgc atctgcttga tttttaagaa ataaatctcc acattctttc
    27781 ttttctaaac ttatttttct aagagatata ttaagaaggt ctttatatag gtttaaaacc
    27841 ttataatcct tttctaaacg tcttcttctc tctgatagtt ccattttatc ataagctaaa
    27901 aaactattta aatcattggc ttttagagag tctaatccct ctttcatagc cttatttaca
    27961 tactttctac attttgctgt agttaactct aaatcttttt ctaattcatc tatttcaggt
    28021 atgtacttat ctactatcca tttgccaata agtggttctg tatttcttgc taatgtctta
    28081 taaaacaata tattaaattt taaaagctct gcatattttg cttcattaaa ttctactttt
    28141 tcaaatttct tttccaaaac accatctagc ttttcattta ttttagactc ttgttttaaa
    28201 aaacttgctg tgacatttaa aacagtgttt cttattatat tattttcact caaaaccttc
    28261 actccttaaa tcaattattt tattgtaata tttacattgt gcaaagttgc taatttgatt
    28321 atgatatcta aataattatt tactaaattt gctacatctt atatcatatc atattattta
    28381 catcatttta ctttttcttt ttatatctat cttatattat accattttta atatctgtac
    28441 taacttatat tacaatattc ctcaaaattt taaaagtaga ttagacaaat ttacaaataa
    28501 aaaaacatca gtttaaactg atgtttttga ctatcgaaat aattaagcaa gtctataagc
    28561 ggagttctgt attagataat catctttcta ggcctattgt caccaatagg ctcaagcgac
    28621 ctacctaccg gagagtacga gcagcactct ttattcctat cttggtcttg ctccaggtgg
    28681 ggtttacata gccaactagt cgcctagctg ctggtgagct cttacctcgc ctttccatcc
    28741 ttaccacaat agtggcggtt tatttctgtt gcactatcct taggatcgct cccactaggc
    28801 gttacctagc accctgctct atggagctcc gactttcctc atgtaagatt acatgcgatt
    28861 atctggctta cttaatattt attttactta gttatgttaa cacaaattct tttacatgtc
    28921 aaagtaatat tatactaaaa atttaaattt attcaaaaat agtgtaatat agatatataa
    28981 actacattta actcgaggtg atctattgaa aaaattatat agaatagtaa ttaacattat
    29041 actagtatta gtaatacttt acagtggatt taatatatac tcaaaactta ccaaatacaa
    29101 tcatgatact aaaatatcct cagaacttca aaaaaaagaa tataaaaaag aggatttatc
    29161 aaagattaac agtgatttta aattttggct ttctgttgag aatacaaata taaattatcc
    29221 agttgtacaa tctaaagata attcttacta tcttgacaaa gatttttata aaaaagattc
    29281 tatttctggt acactgttta tggattatag aaataaatct attgatgaca aaaatatcat
    29341 aatatatgga cataatatga aaaataaaac tatgttcaac aacctaaata agtttaaaga
    29401 tgctgacttc tttaaaaaaa ataataaaat aaaaataacc ttgaatggaa aagaattttt
    29461 atatgatgtt ttttctgctt atattgtaga atctgattat gattatctaa aaacaaattt
    29521 taacaatgaa tctgattatc aaaattacat aaatgatatt acttctaaat cattgtataa
    29581 atctcctatt aaggtaaata gcaatgataa aatagttaca ctttctacat gtacctatga
    29641 atttgatgat gccagaatgg tgattcatgg tagattgatt taaaactcac agacacagtt
    29701 taaaacaata attttaaact gtgtcatctt ttatacctat aatttgtaaa ttagaccact
    29761 aaaagtattt aatacaaaat aacaaataca tcaaacaata aaaaacaata aaaaaagact
    29821 atgctaaaaa catagtcttt actggagctg gtgataggaa tcgaacctac aacctgctga
    29881 ttacaagtca gctgctctac cgttgagcca caccagcaca cctattttat tgtaaattag
    29941 caaaactaat ttttaaaagt ta
//
LOCUS       DAYAPA010000041        29097 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.041, whole genome shotgun sequence.
ACCESSION   DAYAPA010000041 DAYAPA010000000
VERSION     DAYAPA010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 29097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29097
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.041"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(51..1727)
                     /gene="bclA2"
                     /locus_tag="ACGI59_001672"
     CDS             complement(51..1727)
                     /gene="bclA2"
                     /locus_tag="ACGI59_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like exosporium glycoprotein BclA2"
                     /protein_id="HFG9739459.1"
                     /translation="MSDISGPSLYQDVGPTGPTGATGPTGPTGPRGATGATGANGITG
                     PTGNTGATGANGITGPTGNMGATGANGTTGSTGPTGNTGATGANGITGPTGNTGATGA
                     NGITGPTGNKGATGANGITGSTGPTGNTGATGANGITGPTGNTGATGATGPTGLTGAT
                     GATGANGITGPTGNTGATGANGVTGATGPTGNTGATGPTGSIGATGATGTTGATGPIG
                     ATGATGADGEVGPTGAVGATGPDGLVGPTGPTGPTGATGANGLVGPTGPTGATGANGL
                     VGPTGATGATGVAGAIGPTGAVGATGPTGADGAVGPTGATGATGANGATGPTGAVGAT
                     GANGVAGPIGPTGPTGANGVAGATGATGATGANGATGPTGAVGATGANGVAGAIGPTG
                     PTGANGATGATGATGATGANGATGPTGATGATGVLAANNAQFTVSSSSLVNNTLVTFN
                     SSFINGTNITFPTSSTINLAVGGIYNVSFGIRATLSLAGFMSITTNFNGVAQNNFIAK
                     AVNTLTSSDVSVSLSFLVDARAAAVTLSFTFGSGTTGTSAAGYVSVYRIQ"
     gene            complement(2105..2644)
                     /gene="hpt"
                     /locus_tag="ACGI59_001673"
     CDS             complement(2105..2644)
                     /gene="hpt"
                     /locus_tag="ACGI59_001673"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFG9739460.1"
                     /translation="MDIETKKWEVLYSEEKIKDKLRELGAIIEKDYKDKNLMVVSLLK
                     GSFIFCADLVRNINLPLRVNFMTTSSYGNNEESTGRVKVVSDVTTDIAGYDVLVVDDI
                     TDSALTMDFVLKHLKAKNPASLKCCVLLDKPSRRKVDLVPDYCGFEIEDKFVVGYGFD
                     FGDYYRNVPYIFNVTDEDR"
     gene            complement(2972..4279)
                     /locus_tag="ACGI59_001674"
     CDS             complement(2972..4279)
                     /locus_tag="ACGI59_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HFG9739461.1"
                     /translation="MRDIRKELVEMLKAEVKPAVGCTEPVALALACAKAKELLGEEIV
                     ENRMLVSPSIYKNGMCVGIPGTERLGLKIAAALGIVGGHSENGLSVLETLTKEEVKIA
                     EDYMDNTPLSITPADTREKVFIEVVLKGKNHIAKVRIRTKHDNFTFLEKDGEVLLDNE
                     PKVSASNDAAEKAESLMDTVTIQELIKNVEEIDFKDIEFLLDGVKMNEEMAEYGLKQK
                     TGIGVGYGIKKSIEEGLLGNDVINYAMMLTAGASDARMAGVKMPVMSSNGSGNHGLTA
                     ILPIVAYNKKFPQSDERLAKALAISHLVTGYIKNYTGRLSAVCGCGVAASTGATAGIS
                     WLMNGTEKQIEGAIENMIADLSGMICDGAKAGCALKLSSAASAAIQSAIIAKQDCFVP
                     PLNGIVGSSVEQSIQNLGRVSDKGMSITDEIILNVMNDMNKVD"
     gene            complement(4704..6479)
                     /locus_tag="ACGI59_001675"
     CDS             complement(4704..6479)
                     /locus_tag="ACGI59_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFG9739462.1"
                     /translation="MMIMIGLEYIKSDIQNIAEAIVSVLNIDVTIVNKELFRIAGTGV
                     YIDKIGEKVDKYTAFKKSLTEQITILIDDPKSSDICRECYKSGACVEFAEVCCPIICD
                     GDSHGVIGLIAFTKEQAQIIENNKSGLINFLGKMADLISNKLKAQIKTYELELEKKKL
                     ETLLNNMDKAVVSIDVDGKIDKYNSKFKELFNIKDSITGKNIFVELDFIKKPSINNFK
                     KDKSCSFFYKRYGYDSKGIYNINKIVLKNELKGYVIDFIDKRDAIKNYNKMNKDYRIK
                     LENIIGESVAIRNSKKEALIASKSTSTVLITGESGTGKELFARAIHNHSNRVDNPFIA
                     VNCAAIPDNLLESELFGYEEGAFTGAKKGGKLGMFEVAHKGSIFLDEIGDMSLHLQSK
                     LLRVLQEKELNKIGSKSNVSIDVRIIAATNKDLERMVQNGTFREDLYYRLNVIPIKLP
                     SLRERKEDIPLIIKHMVKEYSKKLDKNVIDIDSRVVDAMMEYKWPGNIRELQNIIEYS
                     VNMSSSSIITMNVIPQKIKNTSVEELEVKSDRIIPLDELEKIEIIKALNKYKDYKKDK
                     ELTAKALGISRATLYRKIEKYNIIS"
     gene            complement(6476..6613)
                     /locus_tag="ACGI59_001676"
     CDS             complement(6476..6613)
                     /locus_tag="ACGI59_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9739463.1"
                     /translation="MEINYELIIIVMLFVILMSIQFTLNKIYLILKEIRQILMLKKNK
                     E"
     gene            complement(6691..7260)
                     /locus_tag="ACGI59_001677"
     CDS             complement(6691..7260)
                     /locus_tag="ACGI59_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFG9739464.1"
                     /translation="MKSILCQINKQPVKAKYTSDELECFLDTIEYNVGDKILLVGHIG
                     NLGKRLRGLGTSVTILENSNYEDVCYSLVYNENCNVVKGKLEFLPFDDNQFNKIIIVD
                     QFNVITDCEKASSEIQRVLKGNGEVILEDLNLKNLKVKLNYLKHKFCGSVAKIHYPHE
                     VFNIFSRLNFDGMIKESNKLKYIYVGKRR"
     gene            complement(7444..8079)
                     /locus_tag="ACGI59_001678"
     CDS             complement(7444..8079)
                     /locus_tag="ACGI59_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFG9739465.1"
                     /translation="MIAVKDENNVVNLRGELDNKLEFSHEIFGEKFYNMKIKINRLSD
                     SFDILPMTVSERLLQDIDLDKQNLVNVVGQLRSYNKTLNNKNRLVLTVFVREIKSIDE
                     ENKDPNSIFLDGYVCKEPVYRKTPLGREITDLLVAINRPYNKSDYIPSIVWGRNAKFA
                     KNLKVGDRIQLWGRVQSREYEKKIDENNVVKKMAYEVSISKIKKLDENNNE"
     gene            complement(8747..9628)
                     /locus_tag="ACGI59_001679"
     CDS             complement(8747..9628)
                     /locus_tag="ACGI59_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HFG9739466.1"
                     /translation="MVAILKALYVVFAAIFAVVFGKDVAKANKEGKLEEQSTPKVAVT
                     GFITDFFDTLGIGSFAPTIAMSKALKLNIPDKKMPGTLNVAHTIPVVTEAFIFTSIIP
                     VDGVTLVSMVVAAAVGSYIGAGIIAKMDERKIQLVMGVTLALTAILMLLGHPWINVLP
                     GGGNELGLTGAKLVIGVIGNFILGALMTAGIGLYAPGMAMVYFLGMSAKVAFPIVMGS
                     CALLMPVASMKFIKEDAYTKKASVIIAICGLIGVFVAAYIVKTLPMDILKALVIVVIA
                     YTSATMIMAANKNKKTA"
     gene            complement(9748..10755)
                     /locus_tag="ACGI59_001680"
     CDS             complement(9748..10755)
                     /locus_tag="ACGI59_001680"
                     /EC_number="5.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline racemase"
                     /protein_id="HFG9739467.1"
                     /translation="MKFSRSIQAIDSHTAGEATRIVVGGIPNIKGNSMPEKKEYLEEN
                     LDYLRTAIMLEPRGHNDMFGSVMTQPCCPDADFGIIFMDGGGYLNMCGHGTIGAMTAA
                     IETGVVPAVEPVTHVVMEAPAGIIRGDVTVVDGKAKEVSFLNVPAFLYKEGVEVDLPG
                     VGTVKFDISFGGSFFAIIHASQLGLKIEPQNAGKLTELAMKLRDIINEKIEIQHPTLA
                     HIKTVDLVEIYDEPTHPEATYKNVVIFGQGQVDRSPCGTGTSAKLATLHAKGELKVGE
                     KFVYESILGTLFKGEIVEETKVADFNAVVPKITGSAYITGFNHFVIDEEDPLKHGFIL
                     K"
     gene            complement(10775..11248)
                     /locus_tag="ACGI59_001681"
     CDS             complement(10775..11248)
                     /locus_tag="ACGI59_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050485 - oxidoreductase activity, acting
                     on X-H and Y-H to form an X-Y bond, with a disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HFG9739468.1"
                     /translation="MGISTSMKNTSLHIFRDPLLDLIDNDSDIDLLGVVVVGTSEHNE
                     WKTFLATRLGRWIESLRPDGVIITLDMAGNQHIDFTNAIAEFVKSDIPTVGLTIMGAD
                     GLVITNPYLDKATIIDYKKTTGYIETEVVGDNHMDEVDVKKALAFLKLKMRKDAK"
     gene            complement(11275..11742)
                     /locus_tag="ACGI59_001682"
     CDS             complement(11275..11742)
                     /locus_tag="ACGI59_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050485 - oxidoreductase activity, acting
                     on X-H and Y-H to form an X-Y bond, with a disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HFG9739469.1"
                     /translation="MGIGPSTKETSLHHFRDPLLDIVESDKDVDLLGVIVVGTPDGNE
                     NKTFVGQRTAAWLEAMRVDGAIVSSDGWGNSHVDYANTFEEIGKRDIPVVGVTFNGTQ
                     AKFVVSNQYMDTIVDMNKSKEGIETEVVGENNTNEIDAKKALAFLKLKMRKHG"
     gene            complement(11757..12515)
                     /gene="prdD"
                     /locus_tag="ACGI59_001683"
     CDS             complement(11757..12515)
                     /gene="prdD"
                     /locus_tag="ACGI59_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase cluster protein PrdD"
                     /protein_id="HFG9739470.1"
                     /translation="MEEKIMRRLVIKPFHMNEVNFGSKTSIKKDVLTIDLSSIDEIKE
                     REELITDIKVDIIKPGDYDREINTIMDIIPISTKVLGRLGEGITHTLTGVYVMLTGAD
                     EDGNQMHEFGSSEGILKDQMVFGRYGTPSVEDYIIHVDVTLKGGLPFERTLPLAAFRA
                     CDDFIQEIRASLKMEDGRNATQVREYFDKIRPNAKKVVIVKQIAGQGAMYDNQLFSKE
                     PSGFEGGTSIIDMGNVPMIISPNEYRDGALRAMT"
     gene            complement(12595..13320)
                     /gene="prdB"
                     /locus_tag="ACGI59_001684"
     CDS             complement(12595..13320)
                     /gene="prdB"
                     /locus_tag="ACGI59_001684"
                     /EC_number="1.21.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050002 - D-proline reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(12868..12870),aa:Sec)
                     /transl_table=11
                     /product="D-proline reductase (dithiol) protein PrdB"
                     /protein_id="HFG9739471.1"
                     /translation="MSLTTVQGLQSEIFVPITPPPVWTPVTKELKDMTIALATAAGVH
                     LKSDKRFNLAGDTTFRAIPNTATVDEMMVSHGGYDNGDVNKDINCMFPIDRLHELAAE
                     GFIKGVAPMHYAFMGGGGNQHVFTEETGPAIAAKLKEEGVDGVVMTAGUGTCHRTAVI
                     VQRAIEEAGIPTIIIAALPPVVRQNGTPRAVAPLVPMGANAGEPHNIEMQTHILRDTL
                     EQLVAIPSAGKIVPLPYEYKAHV"
     gene            complement(13358..13642)
                     /locus_tag="ACGI59_001685"
     CDS             complement(13358..13642)
                     /locus_tag="ACGI59_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBO2463/CBO2479 domain-containing protein"
                     /protein_id="HFG9739472.1"
                     /translation="MDRLKYISSETFYEGIIVDIKGGGVTIDLKGRLGQFKIPNRMLI
                     TDYELKIGQEVGFMLSYPEVLSPEPNQEYVENIRREKEKQAEMQSKQEEK"
     gene            complement(13723..15603)
                     /gene="prdA"
                     /locus_tag="ACGI59_001686"
     CDS             complement(13723..15603)
                     /gene="prdA"
                     /locus_tag="ACGI59_001686"
                     /EC_number="1.21.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050002 - D-proline reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-proline reductase (dithiol) proprotein PrdA"
                     /protein_id="HFG9739473.1"
                     /translation="MSITLETAQAHANDPAVCCCRFEAGTIIAPENLEDPAIFADLED
                     SGLLTIPENGLTIGQVLGAKLKETLDALSPMTTDNVEGYKAGEAKEEVVEETVEEAAP
                     VSEAAVVPVSTGVAGETVKIHIGEGKNISLEIPLSVAGQAGVAAPVANVAAPVASAAA
                     EVAPKVEEKKLLRSLTKKHFKIDKVEFADETKIEGTTLYIRNAEEICKEANETQELVV
                     DMKLEIITPDKYETYSEAVLDIQPIATKEEGELGSGITRVIDGAVMVLTGTDEDGVQI
                     GEFGSSEGELNTTIMWGRPGAADKGEIFIKGQVTIKAGTNMERPGPLAAHRAFDYVTQ
                     EIREALKKVDNSLVVDEEVIEQYRREGKKKVVVIKEIMGQGAMHDNLILPVEPVGTLG
                     AQPNVDLGNMPVVLSPLEVLDGGIHALTCIGPASKEMSRHYWREPLVIRAMEDEEIDL
                     VGVVFVGSPQVNAEKFYVSKRLGMLVEAMEVDGAVVTTEGFGNNHIDFASHIEQIGMR
                     GIPVVGVSFSAVQGALVVGNKYMTHMVDNNKSKQGIENEILSNNTLAPEDAVRIMAML
                     KNAIEGVEVKAPERKWNPNVKLNNIEAIEKVTGEKIVLEENEQSLPMSKKRREIYEKD
                     EN"
     gene            complement(16032..17792)
                     /gene="prdR"
                     /locus_tag="ACGI59_001687"
     CDS             complement(16032..17792)
                     /gene="prdR"
                     /locus_tag="ACGI59_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator
                     PrdR"
                     /protein_id="HFG9739474.1"
                     /translation="MFSIPEVKKVEEVMDTKFTTIDEDTRIEDAIKEMIKSNTKTLMV
                     IDSSDQLKGIISMTDIHNLYEMHKKYEGQPVKLIMKKDVIYVNEGLTLDECRDIMILK
                     NIGILPVLRDNKIIGVLKQEHIRDYLYMHLEDYGLTLKYIIGQIKEGICAINNEGVVI
                     LWNKFMEERYDIKSEDIVGRPMNEFLENTISEKVLNSKVGMSDLYFTDKKENMYALVH
                     ANPIFYKEEFIGVVCTEVDVTEAKILALELEKVNDTLKYLKNEVKNLSKGSFDKILGK
                     SYKLEKSKAIAKQVARTNSSIFIWGESGTGKEVFARAIHDYSERKGQFIPVNCSAIPN
                     ELFESEFFGYESGAFTGASKKGRIGIFELAKDGTVFLDEIADLPLSMQAKLLRVLQEK
                     EIRRVGGDTTIKINPRIISATNKDLSKMVKAEKFREDLYYRLNVVEIKIPPLRERKED
                     IGLLVHSFLEEICKQNNKPVLTISKDVIDIFQNYRWKGNIRELKNTIENIVVLSQNSK
                     IDVDDVPSYMMDSTNNSTEEEEYPLDLTKATQKIEIKNITKALKMSNGNKAKAAKILN
                     IPRTTLYYKIDQYKIDVSKI"
     gene            complement(18199..20373)
                     /locus_tag="ACGI59_001688"
     CDS             complement(18199..20373)
                     /locus_tag="ACGI59_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-Gln thioester bond-forming surface protein"
                     /protein_id="HFG9739475.1"
                     /translation="MKTKIKKSSIISLVIAFSMVFTAFTPIVSYADEVTSNDTILNGE
                     EQSNGQTPDVEKPSDGQVPDGEKPSDSQVPDGEKPSDGQVPDGEKPSDGQIPDGEKPS
                     DGQIPDGEKPSDGQIPDGEKPSDGQIPDGEKPSDGQMPDGEKTSDSQTPDVEKPSDGQ
                     MPDGEKPLDEQTPEEEKPLEEEIVIEEMSLKQDIDKILDMTLSQINKIVYNFWEDEED
                     VKADEQSEINQVFTSEDSFISLWYDKKAKVKNSCLLKEIDGSDRPYHDLRFDDITKTL
                     TFDYVIKGLVGASSKDDKYVIEGEDGEQTAFCYNNHLRPPSSNGKSPYLPAEDFNGQE
                     NQNEEAVKSILYAGSEFDGFGYKQQFNLGGEENELMTYSATQSAIWIILGQMDEQERL
                     KQYQGSINLCDKLIERAKTEEERAEYQKKKEVAINIKAYLEALLKAGREELKPNDTGK
                     PSLSNGLTKINFEKNEDGTYETEAVALVGYSGVVKLRLPDGVTAYDGDGNIIGTGEVE
                     ISTQQKFKLKSVVKPDAKANISAVSYDYIFPKAIQYYKAVLDLGQKDHSSSLPSSKQN
                     LLSYTIEKKNGQEVNFNIGLPTDDVVNPPVPPTDDTNGHKPKPSPPIDDTVINPPVPP
                     MDDTIINPPVPPTEDAVLNPPVPPTEDAVLNPPVPPTEDTVLNPPVPSVSDTVEKTPE
                     LSRDDTIIKSPKTGDETQLMSYVFISVIAICGLAYQCKIKRN"
     gene            complement(20528..20656)
                     /locus_tag="ACGI59_001689"
     CDS             complement(20528..20656)
                     /locus_tag="ACGI59_001689"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9739476.1"
                     /translation="MDNQQVSFEKKKLQRLERNLVPTSVGKWGVPNLWLGNDIVCA"
     regulatory      complement(20955..21040)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            complement(21445..22758)
                     /gene="prdC"
                     /locus_tag="ACGI59_001690"
     CDS             complement(21445..22758)
                     /gene="prdC"
                     /locus_tag="ACGI59_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase-associated electron transfer
                     protein PrdC"
                     /protein_id="HFG9739477.1"
                     /translation="MNKLYSILLKQHVGGPDKPVVSVGDVVKKGTLIAEPTGLGANIY
                     ASVSGKISEINDQAIVIEADEVQEDTFEPLKGEGILDLIKEAGVVGMGGAGFPTHVKL
                     NIDLNGGTILANAAECEPLLAHNIKEIEERPEIVYQGIKYAMEVTNAGKGMLAIKSKH
                     PKAIEAFKKVIKPGDNIEVAELVDMYPMGEERAIVRDVLGKLLEPTQLPSEANAVVIN
                     VETLTRIVEAVEQKRPVISKNITVVGQLNSGKESIVFEDVPIGTTVGELIERAGGIKG
                     EYGEIILGGPFTGKATTLDAPITKTSGGIIVTMPFVNEKRKMGLLVCACGPNEERMRD
                     IATKMGVTDIVSVQKCKQAQEIKGALKCENPGHCPGQAQKCIEFKKAGAEVILIGNCT
                     DCSNTVMGSAPKLKLGVYHITDHVMRTVNHPLIRRIKGDVRLKIK"
     gene            complement(23147..23911)
                     /locus_tag="ACGI59_001691"
     CDS             complement(23147..23911)
                     /locus_tag="ACGI59_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFG9739478.1"
                     /translation="MKREVKRTKALIYSVLFFSLLLVLIFVFVYGIKYICSKSDNSKM
                     PIYKVDTNEKKIALSFDVAWGTDNMDDILKILDKHNVKATFFLVGSWVDDNEEIVKAI
                     DEKGHEIGNHSNTHANLKEISKEDITKEIETTSEKIYNITGKRTNLFRPPFGDVNNKA
                     MDICSDLGYKVIKWDVDSIDWKELGPNHVIEKVIKESQPGSIVLFHANINDVDNYLDT
                     IISRLKKDGYSLVKISDLLYKDNYVVDSNGVQKTKN"
     gene            24202..24405
                     /locus_tag="ACGI59_001692"
     CDS             24202..24405
                     /locus_tag="ACGI59_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small, acid-soluble spore protein, alpha/beta
                     type"
                     /protein_id="HFG9739479.1"
                     /translation="MSNSNRTVVPEAKAALNQMKLEIANEIGLSNYENIDKGNLTARE
                     NGYVGGYMTKKLVEMAERQMAGK"
     gene            24596..25132
                     /locus_tag="ACGI59_001693"
     CDS             24596..25132
                     /locus_tag="ACGI59_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4364 family protein"
                     /protein_id="HFG9739480.1"
                     /translation="MFENSSEELAYHKLLILYILEKIKMDLTNSQITQVVLETEMMNY
                     FSLQQLLSQLMESKFLTTYKDSDREYYALTQRGVESLEYFFNRIPTSVTEKIDKYIDS
                     NKENLLADTQVKSSFVKQSDNEFIVNLRVIENQSNLIDLNLNVSSEKQAKLICNNWKN
                     NASYMYAEVIDLLIRDIH"
     gene            complement(25136..26002)
                     /locus_tag="ACGI59_001694"
     CDS             complement(25136..26002)
                     /locus_tag="ACGI59_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HFG9739481.1"
                     /translation="MKIYISNYLSKSISIVDYSTLELEKEIVLEDNIYPHHFCIEKEK
                     NLIYIPSSSNGILYVLDLSNDKIIDTVSIGGSLSQVVLSDNELFVANEDSNSIYVLDK
                     NTLNPIGIIGVDNMPHGFDFDRESKKLYVPCINSIVCIDTINKNIQKKINMDFRAWHI
                     ELDKQKKEMYISTLDGKLIIIDEVSMDIKKVLYEFLLPVEIRFNYSGKKVYVADLGYN
                     NVRILDYTTSRYIGNIEINGIPQGLEISKDEKLLFVSDTQENSVKVYETANNKLIKEI
                     KVGKEPTTIICL"
     gene            26150..26395
                     /locus_tag="ACGI59_001695"
     CDS             26150..26395
                     /locus_tag="ACGI59_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03905 family TSCPD domain-containing
                     protein"
                     /protein_id="HFG9739482.1"
                     /translation="MKVTFNPSGVCCREMIFEVDENNVIVDAEFIGGCNGNLSGLRSL
                     IIGQNALEVADKLNGIDCGGKGTSCPDQLSKAIRQSI"
     gene            complement(26509..27807)
                     /locus_tag="ACGI59_001696"
     CDS             complement(26509..27807)
                     /locus_tag="ACGI59_001696"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HFG9739483.1"
                     /translation="MKYEEALEYISQTNKFGIRLGLENIGKLLELLGNPQETLNIIHV
                     AGTNGKGSVCSFVSNILRECGYKVGLYTSPYLETFTERIRVNGQNIPQDDVARIIELI
                     KEKIEIMVKEGYAYPTEFEVVTAMAFYYYSEQKVDFVALEVGLGGRYDATNIITKSLV
                     SVIVSISLDHTGILGDTIEKIAYEKAGIIKENGVVLVYDQTDEAKDVIKSVCKEKKAK
                     YIEVDFDDINIKKSDINSQIYDCTVMKETYRDLEIKLIGEHQINNSILAISVIKYLKD
                     INKLANINEESIRKGLINTKWPGRIEKIKENPIFIIDGAHNEDGAKSLAKALDKNFKG
                     RKLTLLIGMLEDKDIDSVLEILLSHFNKVITTTPNNPRAINSDILREKVLKYVDDVTS
                     KHEIEDAVNYTLETSSEDDIIISAGSLYMIGTVRTLVKKL"
     gene            complement(27940..28953)
                     /locus_tag="ACGI59_001697"
     CDS             complement(27940..28953)
                     /locus_tag="ACGI59_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HFG9739484.1"
                     /translation="MNNKVKVAVLDTGIDKEHDYLKDNLVGGIAFECMHDYIFISDKF
                     DDEDGHGTACASIIKKEYEDVELFVIKILRKDGITNIKVLEEALRYLLDTNIRLINLS
                     LSVIGVESVKGLFEVCYELFRKGKIIVCSLANDFDLSYPAMFNNVIGVRGFTLDGDDS
                     FWYNKKYDVQCVMDSNSYISCDINNSYRLPPKCNSYLSAKLTGKIAKILSEKPGITIS
                     DLNDKLESLATRNHWDSCDFDECSRIVNFKLDLYDKENALFMKVADVVRDCLDIEENN
                     EKLFKGSLFNKEIGLVYDNCFNLLKKLEHRFDIKFNYMDISKYDLVSIYTLTELVERY
                     KNL"
BASE COUNT         8614 a         5502 c         3364 g        11617 t
ORIGIN      
        1 gtatatgaaa tcatattgtt tattatttta taatcttttc atcatataaa ctattgtatt
       61 ctataaactg atacatatcc agctgcagaa gtacctgtcg tgcctgaacc aaatgtaaaa
      121 cttaaagtaa cagctgctgc tctagcatca actaaaaagc ttaaacttac acttacatct
      181 gatgaagtaa gtgtatttac tgcttttgca ataaagttat tttgagctac tccattaaag
      241 ttagtagtaa ttgacataaa tcctgcaagt gaaagtgtgg cacgtatacc gaaagataca
      301 ttgtatatcc ctccaactgc aagatttata gtactacttg ttggaaaagt tatattagtt
      361 ccatttataa atgatgaatt aaatgtcact aatgtattat tcactaaact tgaagaggac
      421 actgtaaatt gtgcattgtt tgctgctaac actcctgttg ctccggtcgc tccggttgga
      481 cctgttgctc catttgctcc tgttgctccg gtcgcccctg ttgctcctgt cgctccattt
      541 gctccagttg ggcctgttgg tcctatcgct cctgctactc catttgctcc cgttgctcct
      601 actgctccgg ttgggcctgt tgctccattt gcccctgttg ctcctgtcgc tcctgttgct
      661 cctgctactc catttgctcc ggttggacct gttggaccta ttggacctgc tactccattc
      721 gctccggttg ctcctactgc tcccgttggg cctgttgctc catttgcccc tgttgctcct
      781 gtcgctccgg ttggacctac tgctccatct gctcccgttg ggcctgttgc tcctactgct
      841 cctgttggac ctattgcccc agctactcct gttgctcctg tcgctcctgt tggacctacc
      901 aaaccatttg ctccagttgc tccggttggg cctgttgggc ctaccaaacc atttgctcca
      961 gttgctccgg ttgggcctgt tgggcctgtt ggacctacca aaccatctgg acctgttgct
     1021 cctactgctc ctgttggacc tacctctcca tctgctcctg ttgctccggt tgctcctatt
     1081 gggcctgttg ccccagttgt tcctgttgct cctgtcgctc ctatacttcc ggttggacct
     1141 gttgctcctg tatttcctgt tgggcctgta gcacctgtta ctccatttgc tccggttgct
     1201 cctgtatttc ctgttggacc tgttattcca tttgctccgg ttgctccggt tgctccagtt
     1261 agtccggttg gacctgttgc tccagttgct cctgtattcc ctgttggacc tgttattcca
     1321 tttgctccag tcgctcctgt atttcctgtt ggtcctgtag agcctgttat tccatttgct
     1381 ccagttgctc ctttgtttcc tgttggacct gttattccat ttgctccggt tgctcctgta
     1441 ttccctgtcg gacctgttat tccattcgct ccagtcgctc ctgtatttcc tgttggtcct
     1501 gtagagcctg ttgttccatt tgctccagtc gctcccatat ttcctgttgg tcccgttatt
     1561 ccattcgccc cggttgctcc cgtatttcct gttggacctg ttattccatt tgctccggtc
     1621 gctccagttg cacctctagg ccccgtcggt cctgttggac cagtagcacc tgttggccct
     1681 gttggaccta catcttgata taaacttgga cctgaaatat cactcatata attaatcctc
     1741 cttttttaaa gttagagtat tactctataa atattgagta ttactctata taattatatg
     1801 aatcgacaat ttcttatgtg ataatgtatc tatttgctta tatcaacata tatgctttga
     1861 ttcttgaatt tcattacttt tttgtaaaat cttttacaat tattagtact cttttacaat
     1921 tattaataca attaaaaaac tcattttcca tcaattattt aaaatttttt tattatgtta
     1981 taaaaaatag ataattaaat tgcataaaat taatttaata ctactttaat tactttgtaa
     2041 ataaaaaaac ctcaacacga ttaataaaaa tcatattaag gcatccacaa aattatataa
     2101 ttgcttatct atcttcatca gtaacattga atatataagg aacatttcta taataatcac
     2161 caaaatcaaa cccatatcca accacaaact tatcttctat ttcaaatcca caataatctg
     2221 gcactaaatc gacttttctt ctgcttggtt tatccaaaag aacacaacat tttaaactag
     2281 caggattttt agcttttaaa tgttttaaca caaaatccat agtaagtgca gaatcagtaa
     2341 tatcgtctac aaccaaaaca tcatatcctg ctatatcagt agtcacatct gaaactactt
     2401 taacccttcc agtactttct tcattgtttc cataactaga tgtagtcata aaattaactc
     2461 taagtggtaa atttatattt ctaactaagt ctgcacaaaa tataaagcta ccttttaata
     2521 gagaaactac cataagattt ttatctttat aatctttttc tataattgct cctaattccc
     2581 ttaatttatc tttaattttt tcttctgaat acaatacttc ccattttttt gtttctatat
     2641 ccataaaaag acctccaatt gtaataatcc ttcttaattt tataagagat tgctcacttt
     2701 ttcaatactc ttattcaata ttagtcaata ttattgagtt tttatgttca ttcaacaatt
     2761 attaacaatg tatcttatat tttaagtagt tcatatatcc acactaaatt acatactata
     2821 tattttagac aaaaaaagaa aaaacttgca ctaacaagtt ttttctatta tacataaatt
     2881 tatacataat catattttat tagagggtac taattaaatt tatttatgct atttttgtta
     2941 tacctcataa tacaaaacat attataaagt actagtcaac tttattcata tcattcatta
     3001 catttaagat tatctcatct gttatagaca tacctttgtc acttactcta cctaagtttt
     3061 gtatactttg ctcaacactt gaaccaacta taccatttag tggtggtaca aaacaatctt
     3121 gcttagctat tatagcactt tgtatagctg cagatgctgc agaagataat tttaatgcac
     3181 aaccagcttt cgcaccgtca catatcattc cacttaagtc agctatcata ttttctatag
     3241 ctccttctat ttgcttttca gtaccattca taagccaaga gataccagca gttgctccag
     3301 tagaagcagc aacaccacat ccacatacag cagataatct tcctgtatag ttcttaatat
     3361 aaccagttac tagatgagat atagctaaag cttttgctag tctttcatca gattgtggga
     3421 attttttatt ataagcaacg attggaagaa ttgctgttaa tccatgattt ccacttccat
     3481 ttgaactcat aacaggcatt tttacccctg ccattcttgc atctgaagct ccagcagtta
     3541 acatcattgc ataatttata acatcattac ctaaaagacc ttcttctata gactttttaa
     3601 ttccataccc gacaccaata ccagtttttt gttttaatcc atattcagcc atctcttcat
     3661 tcatttttac tccatctagt aaaaattcta tatctttaaa atcaatttct tctacatttt
     3721 ttattaattc ttgtatagtt actgtatcca ttaaactttc agctttttct gccgcatcat
     3781 ttgaagctga tacttttggt tcattgtcta ataatacttc tccatctttt tctaagaacg
     3841 taaaattatc atgtttagtt cttattctca ctttagcaat atgatttttt ccttttaaca
     3901 caacttctat aaatactttc tctcttgtat cagcaggtgt tattgatagt ggagtattat
     3961 ccatataatc ctcagctatt ttaacttcct cttttgttag agtttctaac acacttaatc
     4021 cattttcaga atgtccacct actataccta gagctgctgc aatttttaat cctaatcttt
     4081 cagtacctgg tatacctaca cacataccat ttttatagat actaggacta actaacatac
     4141 gattttcaac tatttcttct cctaataatt cctttgcctt tgcacatgct agagctaagg
     4201 ctactggttc agtacaccct actgctggtt taacttctgc ctttaacatt tccactaatt
     4261 cttttcttat atctctcatt atatataaac ctcccaatta agtttagatt tagtactcaa
     4321 ataattagtt aaataatttc ttatctatat ttatagcaaa atacatgcca tattttttat
     4381 atctttttat atttttagtc ttatattcta taaccattgg tatgactgcc tctatttatg
     4441 tatttttttt atttgtatag agtatttata ttctttaaaa tggagaaaca tatgacaatt
     4501 tcgcaaaaag acaaaaaaaa gtctcaaaat gagattctat atcattttga gacggctcaa
     4561 atatattatt ttttaactta taaaaaccat tttttaattc ataaaaaaag attctgcaaa
     4621 acacttatag tataagttta aagctttctt taaagttatg aatttagaaa aatttaaatt
     4681 ttatatagct ttttattata ttactatgaa attatattgt atttttcaat ttttctatat
     4741 aatgtagctc ttgatattcc aagtgcttta gctgttaatt ctttatcttt tttataatct
     4801 ttatacttat ttaaagcttt tattatctca atcttctcta gttcatcaag tggaattatc
     4861 ctatcgcttt taacttctaa ctcttctaca gaggtattct taattttttg aggaattaca
     4921 ttcattgtga ttattgaaga agaagacata ttcacactat attcaataat attctgcaac
     4981 tctcttatat tccctggcca cttatattcc atcatagcat caacaaccct gctatctatg
     5041 tcaataacat ttttatctaa tttttttgaa tactctttaa ccatatgctt gattataagt
     5101 gggatatctt cttttctctc tcttaaactt ggtaatttta taggtataac atttaatcta
     5161 taatataaat cttctctaaa agtgccattt tgaaccattc tttctaaatc tttatttgta
     5221 gcagcaataa ttcttacatc tattgaaaca tttgatttac ttccaatttt attaagttcc
     5281 ttttcttgta atactctcaa taactttgac tgtaaatgca aactcatatc tcctatttca
     5341 tctaaaaata tacttccctt atgagctact tcaaacattc caagctttcc acctttttta
     5401 gctccagtaa aagctccctc ttcatatcca aataattcac tctctaataa attatccggt
     5461 atagcagcac agtttacagc tataaaagga ttatctactc tattgctatg gttgtgtatg
     5521 gctcttgcaa atagctcctt acccgttcca ctttccccag taataagtac tgttgatgtt
     5581 gatttagaag ctattagggc ttccttttta gaatttctta tagctacact ttctccaatt
     5641 atgttttcca attttattct ataatcttta ttcatcttat tgtagttttt tatagcatct
     5701 cttttatcta taaaatcaat aacatatccc tttaactcat tttttaatac tattttattg
     5761 atgttgtata ttcccttcga atcatatccg tatcttttat aaaaaaaact acaggatttg
     5821 tccttcttaa agttatttat agaaggtttc tttataaaat ctaactcgac aaatatgttt
     5881 ttgccagtta tactatcttt tatattgaaa agctctttaa attttgaatt atatttatca
     5941 atttttccat ccacatcaat agacacaaca gctttgtcca tattgtttaa tagtgtctct
     6001 aattttttct tttctaattc aagctcataa gttttaattt gtgcttttaa tttattagat
     6061 attaggtctg ccattttgcc taaaaagttt ataagaccac ttttattatt ttctataatt
     6121 tgtgcttgtt cttttgtaaa ggctataagg cctataacac catgagaatc gccatcacat
     6181 ataataggac aacacacctc tgcaaactct acacaagccc cacttttata gcattcccta
     6241 catatatcac tactctttgg gtcatcaatt agtattgtaa tctgttctgt aagtgatttt
     6301 ttaaaagccg tatatttatc tactttctca cctattttgt ctatatatac tcctgtacct
     6361 gcaatcctaa ataactcctt atttactata gttacatcta tattgagtac tgatactata
     6421 gcttcagcta tattttgaat atcagatttt atatattcta aacctatcat aatcatcatt
     6481 ccttattttt ctttagcata agtatttgtc ttatttcttt aagaatcaag tatatcttat
     6541 ttaaagtaaa ttggattgac atcaggatta caaagagcat aacaattatt attaactcat
     6601 aatttatttc catctaaaca tctccttata acaaaagact aggcttaacc tagtctttta
     6661 tattatatcc atatttatat tttaactcat ttatcttctt tttcccacat aaatatattt
     6721 taatttattt gactctttaa tcataccatc aaagtttaat cttgaaaata tattaaatac
     6781 ttcatgtgga tagtgaatct ttgcaacaga accacaaaat ttatgtttaa gatagtttaa
     6841 cttaactttt aaatttttta aatttaaatc ctctaatata acctctccat tgccctttaa
     6901 cactctttgt atttcagatg aagctttttc acaatctgta ataacattaa attggtcaac
     6961 tattattatc ttgttgaact gattatcatc aaatggtaga aactctaact tacctttaac
     7021 tacattgcaa ttttcattat acactagaga ataacagaca tcttcataat tactattctc
     7081 cagtatagtt acagaagttc caagacctct caatctcttg cctaggtttc ctatatgacc
     7141 aacaagcaat attttatcac ctacattata ctcaattgta tccaaaaaac attctaattc
     7201 atcagatgta tatttagctt taactggctg tttatttatc tgacataata tgcttttcac
     7261 aatgcttcac ctcttcattt cctcaattat aaattatgta cagattacat tataataaat
     7321 ggcgagcaac tattcaagag atatttcatt agtatttttt taaacaatct ttatacattt
     7381 taatatgata ttataccaaa tttattagga ttttattcat aaaaatcagt ctatttttta
     7441 tgtttattca ttgttatttt catccaattt ttttattttc gatatggata cttcatatgc
     7501 catttttttg accacattat tttcatctat ttttttctca tattctctac tttgaaccct
     7561 accccaaagc tgtattctat caccaacctt tagattcttt gcaaatttag catttctacc
     7621 ccaaactatt gatggtatat aatcagattt attataaggt ctgtttatag caactaataa
     7681 atcggttatt tcccttccta aaggtgtctt cctatacaca ggttccttac atacatatcc
     7741 atctaaaaat atactattag ggtctttatt ttcctcgtct atacttttta tttctctgac
     7801 aaacactgta agcactaatc tatttttatt atttaatgtt ttgttatatg atcttaattg
     7861 gcctacgaca ttgactaaat tttgtttatc aaggtcaata tcttggagaa gtctttctga
     7921 tacagtcatt ggaagtatat caaaagaatc gcttagtctg tttattttta ttttcatatt
     7981 ataaaatttt tctccaaata tctcatggct aaattctagc ttattgtcta actcccctct
     8041 taagtttact acgttattct catctttaac agctatcata ttttatccct ccacaattgt
     8101 taattcttat atttctggta tcagcttata taataaatat atttataagg gatgtatttt
     8161 attacaaatc tttgattgct gtttaaaagt ttttatttag ttataaattt catttaatat
     8221 ttcactttta taattataaa attattatct ttttgtacca catatactaa ttaaactacc
     8281 tatttcagtt ttgtattaat tataaaaatc ctattatata attaataagt catatataac
     8341 ttaaatttat tcactctata taagcaactt caacgaatta aatttattga aataattcag
     8401 atgaatattg agtcattata aacttatttt tcttaattat aagttatatt agacttatta
     8461 caggttatat ttttattaat ctgtattaat atatgaacat ttattgaact tattgattat
     8521 aaaatttaaa tatttagctt taaattatgc tttattcttt aaaaatacaa cgttttttag
     8581 attaaatcta ctaaatcact atatatatta cataattggc atgttaattg ctttatatta
     8641 atttagtaaa taaaaaactg ccctaaaata ttaattcaat attttaaagc agtttataat
     8701 ctttattatt agctgtaata tttaataaac actaagtagt ctttaactaa gcagtctttt
     8761 tgtttttatt tgcagccatt atcattgtag cagaagtata agctataact actattacta
     8821 atgcttttaa tatatccatt ggtagagttt ttactatata tgcagctacg aaaactccta
     8881 ttaaaccaca tatagctatt ataactgatg cttttttagt atatgcatct tcttttatga
     8941 atttcatact tgcaactggc attaataatg cacaagaacc cataactatt gggaatgcaa
     9001 cttttgcaga cattcctaag aaataaacca ttgccatacc tggagcatat agacctatac
     9061 cagctgtcat taaagctcct agtataaagt tacctattac acctattact aatttagcac
     9121 cagtaagacc taattcattt cctccacctg gtaatacgtt aatccaagga tgacctaata
     9181 acattaatat tgctgttaat gctaaagtta cacccataac aagttgtatt tttctctcgt
     9241 ccatcttagc tattattcct gcacctatat atgaaccaac tgctgctgcc acaaccatag
     9301 atactaaagt aacaccatct actgggataa ttgatgtaaa tatgaatgct tctgtaacaa
     9361 ctggaatagt atgtgctacg tttaatgtac caggcatttt tttatctggt atatttaact
     9421 ttaaagcctt tgacattgct attgttggag caaatgaccc tattcctaat gtatcaaaga
     9481 agtctgttat aaatcctgtt acagcaactt ttggagtact ttgttcttct agtttcccct
     9541 ctttgtttgc tttagctacg tctttaccaa acacaactgc aaagattgct gcgaatacta
     9601 catataatgc ctttaagatt gctaccataa tccatcctcc tattttttta ttttttcata
     9661 tattctgaat cttatagcct aaaagctata gaatagcaac cgacaagtgg ctactattct
     9721 attttaattt cctaatttat ttattactta tttaagaata aatccatgtt taagtgggtc
     9781 ttcttcatct attacaaaat gattaaatcc agttatataa gcagaaccag ttattttagg
     9841 tactacagca ttgaaatctg caactttagt ttcttcaact atttcacctt tgaataaagt
     9901 tcctaatata ctttcatata cgaatttttc tcctactttt aattcacctt tagcatgtaa
     9961 agtagctaat ttagcacttg ttccagttcc acatggagat ctgtcaactt gaccttgacc
    10021 aaatataact acatttttgt aagtagcttc tggatgagtt ggctcatcat atatttcaac
    10081 taagtctaca gtttttatat gagctaaagt tggatgttgt atttctattt tctcatttat
    10141 tatatctcta agtttcatag ctagttcagt taatttacca gcattttgag gttcgatttt
    10201 taaacctaat tgacttgcat gtattatagc aaagaagctt ccaccaaatg atatgtcaaa
    10261 tttaacagtt ccaacacctg gtaagtcaac ttcaacacct tctttatata agaaagctgg
    10321 tacgtttaag aatgatactt ctttagcttt tccatctaca actgtaacgt ctcctcttat
    10381 tattcctgct ggagcttcca taactacatg agttacaggc tctactgctg gaactacacc
    10441 tgtttctata gctgctgtca tagctcctat tgtaccatgg ccacacatgt taaggtatcc
    10501 gccaccatcc atgaagatta ttccaaagtc agcatctggg caacaaggtt gagtcattac
    10561 agaaccaaac atatcattat gtcctcttgg ctctaacatt atagcagttc ttaaataatc
    10621 tagattttcc tctaaatatt cttttttctc aggcatagaa tttcctttta tgttaggtat
    10681 tccacctaca actattctag tcgcttcacc tgctgtatga gagtctatag cttgtatact
    10741 tctgctaaat ttcatatcaa attccccctt aggtttattt tgcgtcttta cgcattttta
    10801 attttaggaa agccaatgct tttttaacat caacttcatc catgtgattg tctccaacta
    10861 cttctgtttc tatgtagcct gttgtctttt tataatcaat tatggtagct ttatccaaat
    10921 atggattggt aattactaat ccatctgctc ccataatagt aagacctact gtaggtatat
    10981 ctgattttac aaactctgct atagcatttg taaaatcaat atgctggttt cctgccatat
    11041 ctaaagttat aatcactcca tcaggtcgca atgattcaat ccatcttcca agtctagttg
    11101 ctaaaaatgt tttccattca ttgtgctctg atgtcccaac tacaaccact cctaacaagt
    11161 ctatgtcaga atcattatct atcaaatcta gtaacgggtc tctaaatata tgaagtgatg
    11221 tgtttttcat tgatgtagaa attcccacaa aactcattcc cttacttaaa ttttttatcc
    11281 atgttttctc attttaagtt ttaaaaatgc caaagctttt ttagcatcta tttcatttgt
    11341 gttgttttct ccaactactt ctgtctcaat accttctttt gatttgttca tatcaactat
    11401 ggtatccata tattgattgc ttacaacaaa cttagcttga gtcccattaa atgtaactcc
    11461 aactacaggt atatctcttt ttcctatttc ctcaaatgtg ttagcgtagt caacatgtga
    11521 gtttccccaa ccatctgatg aaactatagc tccatctact ctcatggctt ccagccaagc
    11581 ggccgttctt tgtcccacaa aggttttatt ttcatttcca tctggagttc ctactactat
    11641 tactccaagt aaatctacat ccttatcact ttcaactatg tctaaaagtg ggtctctaaa
    11701 gtgatgaaga gatgtttctt ttgttgaagg tcctattccc ataatatcca cctctcttaa
    11761 gtcatagctc taagggcacc atctctgtat tcattaggag atataatcat aggtacattt
    11821 cccatatcta taattgatgt tcctccttca aatccactag gttcctttga gaataactga
    11881 ttgtcataca tagcaccttg acctgctatt tgttttacta tgacaacctt ttttgcattt
    11941 ggtcgaattt tgtcaaaata ttcacgaact tgagtagcat ttcttccgtc ttccattttc
    12001 aaagacgctc ttatttcttg tataaaatca tcacatgctc tgaatgctgc aagtggaagt
    12061 gttctttcaa aaggtaaacc tccctttaaa gttacatcta catggattat ataatcttca
    12121 actgatggag ttccatatct cccaaatacc atttggtctt tcaaaattcc ttctgaagaa
    12181 ccaaattcat gcatttgatt tccatcttca tctgcaccag ttaacattac atatacaccc
    12241 gttaaagtat gtgtaatacc ctctcccagt ctgcctaaaa cttttgttga tattggtata
    12301 atgtccataa tagtattaat ttctctgtca taatctccag gttttataat gtctactttt
    12361 atgtctgtta ttaactcttc tctttctttt atttcatcta tacttgataa atctattgta
    12421 agtacatctt tctttataga agtttttgaa ccgaaattaa cttcattcat atgaaacggt
    12481 tttataacaa gtcttctcat tattttttct tccataatat ccacctttgt tcttaaaacc
    12541 cctctcctaa ccgtgtagga gaagggttct atattttcaa atataaatta cattttaaac
    12601 gtgagcttta tattcgtatg gtaatggaac tatcttacca gcagatggta ttgcaactaa
    12661 ttgctctaat gtatctctta atatatgtgt ttgcatttct atattatgtg gttcaccagc
    12721 attagcaccc attggaacta atggagcaac tgctctagga gttccgtttt gtctaactac
    12781 tggaggaaga gctgctatta ttattgtagg tattccagct tcctctattg ctctctgcac
    12841 gatcacggca gttctatggc aagtacctca gccagctgtc ataactacac cgtctactcc
    12901 ctcttcctta agtttagcag cgatagcagg accagtttct tcagtgaaga catgttggtt
    12961 tcctccacca cccatgaatg catagtgcat aggagctact cctttgataa atccttctgc
    13021 agctaattca tgtaatctat ctatagggaa catacagttt atatctttgt ttacatctcc
    13081 attgtcatat ccaccatgtg ataccatcat ttcatcaact gtagctgtat taggtatagc
    13141 tctaaatgta gtatctcctg ctagattaaa tctcttgtca gattttaaat gaacaccagc
    13201 tgctgtagct aaagctatag tcatatcttt taattcttta gttacaggag tccaaactgg
    13261 aggaggtgtt attggaacga atatttcaga ttgaagtcct tgtactgttg taaggctcat
    13321 atatttcctc tcccttctat tttcaaacga ttatttatta tttttcttct tgttttgatt
    13381 gcatttctgc ttgtttttct ttttctctcc taatattttc tacatactct tgattaggct
    13441 ctggacttaa tacctcagga tagcttagca tgaatccaac ttcttgacct atcttaagtt
    13501 cgtagtcagt tataagcatt ctattaggta ttttaaattg tccaagtcta ccttttaggt
    13561 ctatagtcac tccaccgcct ttgatgtcaa ctataatacc ttcataaaaa gtctctgatg
    13621 agatatattt taacctatcc attataattc tacctctcta tactgtttta aacttggatc
    13681 taaatttatg ttattgtcta aatcttagtt agtatctaaa ttttagtttt cgtctttttc
    13741 gtatatttct cttctcttct tactcattgg tagagattgc tcattctctt ctaatactat
    13801 tttttctcct gtaacttttt ctatagcttc tatgttattt aatttaacat ttggattcca
    13861 ttttctttca ggagctttaa cttctacacc ttctatagca tttttaagca tagccattat
    13921 tctaacagca tcttctggag ctaaagtgtt gttagataat atttcattct ctataccttg
    13981 cttagactta ttgttgtcta ccatgtgagt catgtattta ttaccaacaa ctagagcacc
    14041 ttgaacagct gagaaactta caccaactac tggtatacct ctcataccta tttgctctat
    14101 gtgagatgcg aaatctatat ggttgtttcc gaaaccttca gtagttacta cagctccatc
    14161 aacttccata gcttcaacta acatacctaa tctcttagat acatagaatt tctcagcatt
    14221 tacttgtgga gaaccaacaa atacaacacc tactaaatct atttcttcgt cttccatagc
    14281 tcttattact aatggctctc tccagtaatg tcttgacatt tcttttgatg caggtcctat
    14341 acaagttaat gcatggatac caccatctaa tacttcaagt ggagataata caactggcat
    14401 gtttcctaag tcaacgtttg gttgagctcc taatgtacca actggctcaa ctggtaatat
    14461 taggttatca tgcattgcac cttgtcccat tatttctttt ataacaacaa ctttcttttt
    14521 accttctctt ctgtattgct ctattacttc ttcatcaact actaaagagt tgtcaacttt
    14581 ctttaatgct tctcttattt cttgagttac atagtcaaat gcacggtgag cagctaaagg
    14641 tcctggtctt tccatgttag ttcctgcttt tattgttact tgacctttga tgaatatttc
    14701 acctttgtca gcagcacctg gtctacccca cattatagta gtatttaact caccttctga
    14761 agaaccaaat tcacctattt gaactccatc ttcatctgta ccagttaata ccattacagc
    14821 tccatctata actctagtta tacctgaacc taattcgcct tcttctttag tagcgattgg
    14881 ttgtatatct aatacagctt cactgtaagt ttcatattta tcaggagtta ttatttctaa
    14941 cttcatatct acaactaact cttgagtttc attagcttct ttacatattt cttctgcgtt
    15001 tctgatgtat aaagtagttc cttctatttt agtttcatca gcaaattcaa ctttatctat
    15061 tttaaagtgt tttttagtta agcttcttaa aagtttcttt tcttcaactt ttggagctac
    15121 ttctgctgct gcacttgcca ctggagcagc aacgtttgct actggagcag caactcctgc
    15181 ttgaccagct actgataaag gtatctctaa gcttatgttc ttaccttcac ctatgtgtat
    15241 tttaactgtt tcacctgcaa ctcctgtgct tactggaact actgctgctt ctgatactgg
    15301 agctgcttct tctactgttt cttctactac ttcttcttta gcctctcctg ctttgtatcc
    15361 ttctacgtta tctgtagtca ttggagaaag tgcatctaaa gtttctttta acttagctcc
    15421 tagtacttga cctatagtta atccattttc tggtattgtt aataatccag aatcctctaa
    15481 gtctgcaaat attgctggat cttctaagtt ttctggcgct ataattgttc ccgcttcaaa
    15541 tctacaacaa caaactgctg ggtcatttgc atgggcttga gctgtttcta aagttattga
    15601 catagtataa aatacctcct tacattattt tcttatttat tattttaaat ctcaattcct
    15661 caacaggtaa ccccttaact gagaaattaa gtaaattaaa acctacataa tttgttatga
    15721 ttaatattat ttttttctca cttaaagtca ctttatatta taagcaattc ctatgccaac
    15781 ttattaacaa aaagattttt tctataatag attcttcatc tatcgctatt tttagccaaa
    15841 tattatataa atatttattt aaaatatgtt attttttgta tttgtaaaca ttttctcaaa
    15901 caaaaaaagt gtcaattaat caacactttt tcattttttt taacacatct atatacacaa
    15961 cacttatata agcgttggct ttactaggtt ttaaattaaa gtttatcatt ttgtcaaaat
    16021 ttagtcatgt gtcatatttt agacacatct attttgtatt ggtctatctt ataatacaaa
    16081 gttgttctgg gtatatttaa tatcttagct gctttagctt tatttccatt agacatttta
    16141 agtgccttgg ttatattctt tatttcaatt ttttgagttg ccttcgttaa atctaaagga
    16201 tactcttctt cttcagtact attatttgta gaatccatca tataacttgg tacatcatcc
    16261 acatctattt tagaattttg tgacaataca acaatatttt caatcgtatt ttttaattct
    16321 ctaatattac ctttccatct ataattctga aatatatcta ttacatcttt gctaattgta
    16381 agaacaggct tattattttg tttacatatc tcctctaaaa agctatgaac caataatcct
    16441 atgtcttctt ttctctctct aagtggagga atctttattt caacaacatt taatctatag
    16501 tataaatctt ctctaaattt ttctgccttt accattttac tcaaatcttt attggtagct
    16561 gatattattc taggatttat ttttattgtg gtatctccac caactcttct tatttctttt
    16621 tcctgtaata ctctaagcaa ctttgcttgc atactaagag gtaaatcggc aatttcatct
    16681 aaaaatacag ttccatcttt tgcaagttca aatataccta ttcgaccctt tttacttgct
    16741 cctgtaaacg cacctgattc atacccaaaa aactcacttt caaataactc atttggtatg
    16801 gcactacagt taacaggtat aaattgacct tttctctcac tataatcatg aatagctctt
    16861 gcaaatactt ctttacctgt accactttcg ccccaaataa atatgctact atttgttcta
    16921 gccacttgtt ttgcaatcgc tttcgatttt tccaatttat aacttttacc taaaatctta
    16981 tcaaaactac ctttagataa atttttgact tcgttcttta gatattttaa tgtatcattt
    17041 actttttcaa gttcaagagc taatatctta gcttctgtaa catcaacttc tgtacataca
    17101 acaccgataa attcttcttt ataaaatata ggatttgcat gaactaatgc atacatattt
    17161 tcttttttat ctgtaaaata caagtcactc attccaactt tactatttag tactttttct
    17221 gaaatagtat tttccaaaaa ttcattcatc ggtcgaccaa ctatatcttc agactttata
    17281 tcataccttt cctccataaa cttattccac aagataacaa caccttcgtt atttatagca
    17341 caaattcctt ctttaatttg acctataata tacttcaaag ttaatccata atcttctaaa
    17401 tgcatatata gatagtccct aatgtgctct tgttttagaa ctccaataat tttattatct
    17461 ctaagaacag gaagaatccc tatatttttt aatatcatta tatctctaca ttcatctaag
    17521 gttaaacctt cgttgacata tattacatcc ttcttcatta taagttttac tggttgacct
    17581 tcatactttt tatgcatctc ataaagatta tgtatatccg tcatggatat aatgcctttt
    17641 aattggtcag atgaatctat taccattaga gtttttgtgt tgctttttat catttctttt
    17701 attgcatctt caattcttgt gtcttcgtct atagttgtaa attttgtatc cattacctct
    17761 tctacttttt ttacttctgg tatcgaaaac ataaagtccc ccctttatag tagtacctca
    17821 tcaaaactta tttatatata tttattctag catattataa taattattta ttaaataatt
    17881 attattaaaa tttattaagt aattagcatt taaatttata taatcacttt aaaatttata
    17941 attaaattta tacaaatgaa atcaaatgaa ttataccctt agtatattat tttttcaata
    18001 ctattttcac cttcaaaaca tttttctatt catcaaacaa ttaatgaatg cctgtatcca
    18061 atcataaatt taagaatata attaaaatat atatttttaa acaaactata tttgcatatt
    18121 atatttacat aaaaaagccc caaaatgact tataaatcaa tttgagactt tttattttaa
    18181 acttaataat aatctagatt aatttctttt tattttacat tgatacgcta atccacaaat
    18241 tgcaatgaca ctaataaata catatgacat tagctgagtt tcatcacctg tttttggtga
    18301 ttttattatt gtatcatctc ttgatagttc tggagttttt tccactgtat cacttactga
    18361 tggtactggt ggatttaaca ctgtatcctc tgttggtggc actggtggat ttagcactgc
    18421 atcctccgtt ggtggcactg gtggatttaa cactgcatcc tccgttggtg gtactggtgg
    18481 atttattatt gtatcatcca ttggtggtac tggtggattt attactgtat catctattgg
    18541 tggtgatggt tttggcttat gtccatttgt atcatctgtt ggtggcactg gtggatttac
    18601 aacatcatct gttggtagac ctatattaaa gttaacttct tgaccatttt ttttctctat
    18661 tgtataactt aataaatttt gcttactaga tggtaatgaa gatgaatggt ctttttgtcc
    18721 taaatcaagc actgccttat aatattgaat agcttttgga aaaatataat cataagatac
    18781 agcagaaata ttagctttgg catctggttt tacaacactc ttaagtttaa atttctgttg
    18841 tgttgaaatt tctacttcac ctgtacctat tatattacca tctccatcat aagcagttac
    18901 tccatctggt agtcttaact ttactacacc actataacct actaaggcta cagcttctgt
    18961 ttcataagta ccatcttcat ttttttcaaa gttaatctta gttaaaccat tactcaaact
    19021 aggttttcct gtatcatttg gctttaactc ttcacgacct gcttttaaca atgcttctaa
    19081 ataagctttt atatttatag ctacttcttt cttcttctga tactcagctc tttcctcttc
    19141 agtttttgct ctttctataa gtttatcaca aaggtttata gaaccttgat actgcttaag
    19201 tctttcttgt tcatccattt gtccaagaat aatccaaatt gcactttgag tagctgagta
    19261 agtcatcaat tcattttctt ctccacctaa attaaattgt tgcttatatc caaatccatc
    19321 aaattcagaa cctgcatata gtatagattt aactgcttct tcattttggt tttcttgacc
    19381 attaaaatct tctgctggta aatatggact ctttccatta ctacttggtg gtcttaaatg
    19441 attattgtaa caaaatgcag tttgctctcc atcttcacct tctattacat atttgtcgtc
    19501 ttttgagctt gctcctacaa gaccctttat tacatagtca aaagttaaag tctttgtaat
    19561 atcatcaaat cttaaatcat gataaggtct atctgaacca tctatttctt ttagtaaaca
    19621 agaatttttt acttttgctt ttttgtcata ccataagcta ataaatgagt cttctgaagt
    19681 aaaaacttgg ttaatttctg attgttcatc tgcctttaca tcttcttcat cttcccaaaa
    19741 gttatatact attttattta tttgagataa agtcatatca agaattttat caatatcttg
    19801 ttttaaactc atttcctcta taactatttc ttcttccaat ggtttttctt cttctggagt
    19861 ttgctcatct aatggtttct ccccatctgg catctgacca tctgatggtt tctctacatc
    19921 tggcgtctga ctatctgatg tcttctctcc atctggcatc tgaccatctg atggtttctc
    19981 cccatctggt atctgaccat ctgatggttt ctctccatct ggtatctgac catctgatgg
    20041 tttctctcca tctggtatct gaccatctga tggtttctcc ccatctggta tctgaccatc
    20101 tgatggtttc tccccatctg gtacctgacc gtctgatggt ttctccccat ctggtacttg
    20161 actgtctgat ggtttctccc catctggtac ctgaccgtct gatggtttct ctacatctgg
    20221 tgtctgacca tttgattgtt cttctccatt aagtattgta tcattgctag tcacttcgtc
    20281 agcataagat actattggtg taaatgcagt aaacaccata ctaaaagcaa ttactaaact
    20341 gattatactt gattttttaa tttttgtttt caaaattctc ctgatactaa aaacatatat
    20401 tgtattcact taatataatt aaaattatta aatattatat gaatcttatt caataataca
    20461 tctttagtgt gttcaattag ggtcgttact cctaaccagt tctcctatga acttcttaat
    20521 atttctatta agcacagact atatcattac ctaaccataa attaggtact ccccatttcc
    20581 ccacacttgt aggtacgaga tttctctcta gtcgttgaag tttcttcttt tcgaagctta
    20641 cctgctgatt atccattttc aaggcactta ggatttaacc ttatgctatc taactaattt
    20701 tttctacttt cgtaacctca agtgcttatt aaatgcacac tctatcacac ttatccttat
    20761 ttcatgatta tgttgtggtt tagttagctt taggaacttc cagcaattaa aggagttttg
    20821 ggcagatatc tgccactcta cactcgttat gaatgcaggg agcatttcaa aaatatattt
    20881 atatctatct tctattaatt acttatttat gtatttattt tatatatttt tgacaattaa
    20941 aagttactcc tatttatatt tatagcaggt tgcactacta actccatata tcttttaagt
    21001 gcccaggttt aagatatact tcacagtttc tataaatagg ttttttaatt tttctttttt
    21061 aactttttaa gtgcactttt ttaacaactc aataaaatat aaattagtat cctagatact
    21121 tatctaataa aagacaaaaa ttagttttta taataaaaaa gatactatag agtatcttca
    21181 aatatcacaa aaacctttta ctctatcgga tagttgactt aatataatat gcatattaat
    21241 acaaaccaca tacttgagta tcattatttt tgtccaatat taattgtaat agctattatt
    21301 atcattatac aatatctagc atattttgtc tattttttcc atttatttta caatatatat
    21361 attgccctca aaatacatta aaaaacatcc ttagaagtaa cttttaagtt ttacttctaa
    21421 agatgtctaa cttctattta tctattattt tatttttaat cttacgtcac cttttattct
    21481 tcttattaat gggtgattaa ctgttctcat tacatggtca gtaatatgat atacacctaa
    21541 ttttaatttt ggtgcagaac ccattactgt gttactacag tcagtacagt ttcctattaa
    21601 tataacttct gctccagctt tcttaaattc aatacatttt tgagcttgtc ctggacagtg
    21661 tcctggattt tcacatttta atgcaccttt aatttcttga gcttgtttac atttttgaac
    21721 tgatactata tcagtaactc ccattttagt tgctatatct ctcattcttt cttcattagg
    21781 tccacatgca caaactaaaa gacccatttt tctcttttca ttaacaaatg gcatagtaac
    21841 tattatacca ccactagttt tagttattgg agcatctaaa gtagtagctt taccagtgaa
    21901 aggtcctcct aggattattt caccatattc accttttatt ccaccagctc tttcaattaa
    21961 ttctccgaca gttgttccaa ttggcacatc ttcaaacact atagattctt ttccactgtt
    22021 taattgacca actacagtta tatttttaga tataactggt cttttttgct caacagcctc
    22081 tactattcta gttagtgttt ctacgtttat tactactgca ttagcctctg aaggtaattg
    22141 agttggctca agtaattttc ctagaacgtc tcttactatt gctctctctt ctcccattgg
    22201 gtacatatca actagttctg caacttctat gttgtctcct ggctttatta cttttttgaa
    22261 agcttctatt gcttttggat gcttactttt tattgctagc ataccttttc cagcattagt
    22321 aacttccata gcatatttta taccttgata aactatttct ggtctttctt ctatttcttt
    22381 tatattatga gctaataaag gttcacattc agctgcattt gctaatattg ttccaccatt
    22441 taaatctatg tttaatttta catgagtagg gaatcctgct ccacccattc ctactactcc
    22501 agcttcttta attaagtcaa gtataccttc tccttttaat ggttcaaaag tatcttcttg
    22561 tacttcatca gcttcaatta ctatcgcttg gtcatttatt tcacttattt ttccactaac
    22621 actggcataa atatttgccc ctaatccagt tggttctgcg attagagttc ctttttttac
    22681 tacatctcca acacttacta ctggcttatc tggaccacca acgtgttgtt ttaataaaat
    22741 agaatatagt ttgttcaact agtacgcccc cttaagtata ttaatttttt tataagctgt
    22801 tcaaataatg ttaatgctat ttttctatta taaggtctta acatattgtt tatttctata
    22861 gaacatatcg cttattaaca aagcaatttc tatgccaact tttaaacaaa cttataccct
    22921 ttggaaatac tgtcttacag cattcattaa ttcttctaaa tttttaaacc catcaaaaaa
    22981 aagtcaatta ttagacactt ttttaatcaa tttaatctat aattattgat attgcataca
    23041 tgtataaaaa gtgtcaaaaa atagacaccg tctttttatc gacacttttc taacatatac
    23101 tatataattt atcacaactt tacttaaaat atacatttat tacttattaa ttcttagttt
    23161 tttgcactcc attggaatca actacataat tatctttata caacaaatca gatattttga
    23221 ctagactgta tccatctttt tttagtcgac ttattatagt atccaaatag ttatctacat
    23281 catttatatt agcatggaat aaaactattg aaccaggttg agattctttt attacttttt
    23341 caattacatg atttggtcca agctccttcc agtctataga atctacatcc cacttaatta
    23401 ctttataacc taaatcacta caaatgtcca tagctttatt gtttacgtcc ccaaaaggag
    23461 gtctaaataa attagtcctt tttcctgtta tattatatat tttttctgat gtagtttcta
    23521 tctcttttgt tatgtcttct tttgatattt ccttcaaatt ggcatgagta tttgaatgat
    23581 ttccaatttc atgtcctttt tcatctatag cttttacaat ttcttcatta tcatccaccc
    23641 aactaccaac taaaaagaag gtagctttta cattatgttt atctaaaatt tttaaaatat
    23701 catccatatt atcagttccc catgctacat caaaactcaa agctattttt ttctcatttg
    23761 tatcaacttt atatataggc atttttgaat tgtcagattt agaacatatg tattttattc
    23821 cataaacaaa aacaaatatt aacactaata ataatgaaaa aaataaaaca ctatatatca
    23881 aagctttagt ccttttaact tctcttttca tactattccc cctttgaaat aatttcttct
    23941 atatttaaaa tttatttttt ttcagctcaa aaaagtactg taatatttaa tttataagac
    24001 aataactaaa aaaataaaac ctatattgac ctaattaaac tctctaaaat actcatttat
    24061 ttaatttttt tatatttttt acatttttac ctttattttt ctgtatatat tatttttatt
    24121 ttggagaaat tagtattaac atcacataaa taaaagtgtt gttagacatc ataaactaat
    24181 tatttgagga ggaaatttac tatgtcaaat tcaaacagaa cagtagttcc agaagcaaaa
    24241 gctgctttaa accaaatgaa attagaaata gctaacgaaa taggtttatc taactatgaa
    24301 aacatagaca aaggtaactt aacagctaga gaaaatggat atgttggtgg atatatgact
    24361 aagaaattag ttgaaatggc tgaaagacaa atggctggaa aataaatagt aaattttgac
    24421 acctgggtat caacagggct gctaattagc agccctgttt ttctatattt atttataaaa
    24481 ttgtttttat atgaattatt aaagattaat aatacaatac aaattgttct tttaattttg
    24541 tacaaattag tttaatatat tatatataga aatagtttga cggaggatga tatttatgtt
    24601 tgaaaactcg tctgaagaat tagcctatca caaactttta attttatata tattagaaaa
    24661 aattaaaatg gatttaacta attctcaaat aactcaagta gtattagaga ctgagatgat
    24721 gaattatttt tcattacaac agcttttatc tcagcttatg gaatctaagt ttttgactac
    24781 atataaagac tctgacagag aatattatgc tcttactcaa agaggcgtag aaagtctaga
    24841 atacttcttt aatcgaatac ctactagtgt tactgaaaaa atagacaagt atatagattc
    24901 taacaaagaa aatctactag cagatactca agtaaagtct agttttgtaa aacaaagtga
    24961 taatgaattt attgtcaacc ttagagtcat agaaaatcag tctaacttaa tagatttaaa
    25021 tctaaatgtt tcatcagaga aacaagcaaa acttatttgc aataactgga aaaataatgc
    25081 atcttatatg tatgccgaag ttatcgattt acttataaga gacatacatt aaagcctaca
    25141 aacaaattat agttgtaggc tcttttccta ccttgatttc ttttattaat ttgttatttg
    25201 cagtttcata aacttttact gaattttctt gtgtatctga tacaaatagt aacttttcat
    25261 ctttagatat ttctaatcct tggggaatcc catttatttc aatatttcca atgtatctac
    25321 tcgttgtata atctaaaatt cttacattat tataacccaa atcagcaaca taaacttttt
    25381 tgccactata attaaatctt atttctactg gtaataaaaa ttcatataat acctttttta
    25441 tatccatgct tacttcatct attattataa gctttccatc aagtgttgat atatacattt
    25501 ctttcttttg tttatcaagt tctatatgcc aagctctaaa gtccatattt attttctttt
    25561 gtatattttt attaattgta tctatacata ctatcgagtt tatacacggt acatataact
    25621 ttttgctttc tctatcaaag tcaaatccat gtggcatatt atcaactcca ataattccta
    25681 ttggatttaa tgtgttttta tctagtacat atatactatt agaatcttca tttgccacaa
    25741 acaattcatt atcgctaagt acaacttgac taagacttcc tcctatagag acagtatcaa
    25801 ttattttatc attacttaaa tctaagacat agagtatccc attacttgaa ctaggtatgt
    25861 atattaaatt tttttctttt tctatgcaaa aatggtgtgg atatatatta tcttctaata
    25921 ctatttcttt ttctagttca agtgtagaat aatcaactat gctaatactc tttgataagt
    25981 agttagaaat atagattttc atataacccc tcctctctac aatacatata ctacaataat
    26041 atattatttc attagcttgt attatgcttt atagttgcat tattacgcat atatttggta
    26101 ttattattaa tgttaaaaaa agaaataata tttaatggag gtctttttaa tgaaagtaac
    26161 atttaatcca agtggtgtat gttgtagaga aatgattttt gaagttgatg aaaataatgt
    26221 gattgttgat gctgagttta taggaggatg taatggtaat ttatcaggac ttagaagctt
    26281 aataattggc caaaatgctc ttgaagttgc tgataaatta aatggtattg actgtggtgg
    26341 aaaaggtact tcttgcccag accaattatc taaagctata cgtcaatcta tataattact
    26401 tatataaaca tcagtaaaat tattaaaaac ttattttata taaaaagagt tgtccatttt
    26461 ttagacaact cttttttata tttttatttg tatacttatt ttataaaatt ataatttctt
    26521 tactaaagtt ctcactgtac caatcatata taatgaacct gcacttatta taatatcatc
    26581 ctcacttgat gtttctaatg tataatttac tgcatcctct atctcatgct ttgatgttac
    26641 atcatctaca tattttaata ctttttctct taatatatct gaatttatgg cccttgggtt
    26701 atttggtgtt gttgttatta ccttattaaa atgtgacaac aatatctcta aaacactatc
    26761 aatatcttta tcttctaaca tacctattaa taaggttaat tttctacctt taaaattctt
    26821 atcaagtgct tttgccaaag attttgctcc atcttcatta tgtgcaccat ctattataaa
    26881 aattggattt tcctttatct tttctattct tcctggccat ttagtattta taagtccttt
    26941 tcttatagat tcttcattta tatttgctaa tttatttatg tcttttagat attttattac
    27001 actaattgcc aagatagaat tgtttatttg atgttcgcct atcaatttta tttcaaggtc
    27061 tctataagtt tctttcatta cagtacaatc gtatatttga gaatttatat ctgatttttt
    27121 tatgtttata tcatcaaaat caacttctat atatttagct tttttctctt tacaaactga
    27181 ctttataaca tcctttgctt catcagtctg gtcatatact agtactactc cattttcctt
    27241 tattattcca gccttttcgt acgctatttt ttctattgta tctccaagta tacctgtatg
    27301 gtccaaactt atagatacta tcacacttac aagtgatttt gttattatat ttgtagcatc
    27361 atatctgcca cctaaaccaa cttctaatgc cacaaagtct accttttgtt ctgaatagta
    27421 ataaaatgcc attgcagtaa ccacttcaaa ttctgtcgga tatgcatagc cttcttttac
    27481 cataatctct attttttctt ttattaactc aattattcta gcaacatcat cctgtggtat
    27541 gttttgtcca tttactctta tacgctcagt aaaagtttct aaatatggag atgtatataa
    27601 accaacttta tatccacact ctcttagtat attagaaaca aaagaacaca cagaaccttt
    27661 tccattagtt ccagctacat gtattatatt caaagtttct tgtggatttc ctaataattc
    27721 taataacttt ccgatatttt ctaatcctaa tcttataccg aatttattag tttgtgatat
    27781 gtactctaat gcttcttcat atttcattat ctaactcccc ctgtattggc attttctctt
    27841 atgctatcta attatagtat attttttact tttaatctac cgacaaagaa tacatataat
    27901 ttaatacctt ctacattaaa tttagctaca aatctttatt tataagtttt tatatctttc
    27961 cactaattca gtcagtgtat atattgaaac taagtcatat tttgagatgt ccatataatt
    28021 aaactttatg tcaaatctat gctctagctt tttcaacaga ttaaaacaat tatcataaac
    28081 caagcctatc tccttattaa aaaggctacc cttaaagagt ttttcattat tctcttcaat
    28141 atctaagcaa tccctaacaa cgtcagcaac tttcataaaa agagcatttt ctttgtcata
    28201 caaatccaat ttaaagttta caattctact acactcatca aaatcacaac tatcccaatg
    28261 atttctagtt gctaatgatt caagcttatc atttaaatca gatattgtta tgcctggttt
    28321 ttctgataat atcttagcta ttttaccagt aagctttgct gataaataac tattacattt
    28381 tggtggtaat ctataggaat tatttatatc acaacttata tatgaattgc tatccataac
    28441 acattgaaca tcatattttt tgttgtacca aaatgaatca tctccatcta aagtaaatcc
    28501 tcttacacct ataacattgt taaacatagc tggataactt aagtcaaaat cattagcgag
    28561 tgaacatact attatttttc cttttctaaa caattcatag caaacttcaa ataagccctt
    28621 aacactttct actcctatga ctgataaact taaattaatt aatcttatgt tagtgtctaa
    28681 aaggtatctt aatgcttctt caagaacttt tatatttgta ataccatctt ttctcaaaat
    28741 ttttattaca aataactcta catcttcata ctctttcttt attattgatg cacaagcagt
    28801 tccatgacca tcttcatcat caaatttatc tgatataaag atataatcat gcatacattc
    28861 aaaagcaatt cctccaacta aattatcttt taagtaatca tgttctttgt ctattcctgt
    28921 atctaatact gcaaccttaa ctttattatt catatcacat atattctcca ttacaaaagt
    28981 ttttaatata tctatgttaa tagttcatct taaactttga tttaagttgc acaatttatc
    29041 ttttaaatgc tatatttctt gctaaaaaca taaaataagt acaccatata tttaatt
//
LOCUS       DAYAPA010000042        28850 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.042, whole genome shotgun sequence.
ACCESSION   DAYAPA010000042 DAYAPA010000000
VERSION     DAYAPA010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 28850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28850
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.042"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            826..2496
                     /gene="pdcB"
                     /locus_tag="ACGI59_001698"
     CDS             826..2496
                     /gene="pdcB"
                     /locus_tag="ACGI59_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase PdcB"
                     /protein_id="HFG9739485.1"
                     /translation="MREESNSLSLDHIEIFFELLSQSTEDYIFFWDINRNKFKISSAI
                     FDEFNLSKEIESDLVNCWSKIVYPDDVQIWKDDIQELLHGKKGEHNLEYRLINKYKEI
                     VWISCRGKVYVSDDPKTIFLVGRIKNIGEKNKFDSITGTWNREQFEHRMNYLIKEKIY
                     KNGAMFIMDIDNFKNINEKYGHSYGDKVLRAIATEVLEYLPKDVRLYRLDGDEFAFFY
                     PMCTKETIEKIYEKIQMYTNTQHEIESNKYYCTVTAGVAMYPEDGDNYLDLFKHADIA
                     LDIAKISGKNRIKFFSQELYENKLKVISMQQKLRECVENNFNDFELFFQPQVNAVTKE
                     VIGAEVLLRWHSSTYGEVSPVEFIPILEQSNLIIPVGKWIIKEAVKQCKEWHKINPDF
                     KISVNVSYIQLKEDFFRDFIVECLVEYQLRPEFLILELTENCWIPDINLLNDKFISLK
                     GIGVYIAIDDFGTGYSSLNYLKELSVNIIKIERSFVKNITYNSYEYTFLEYIIKLAHI
                     INLKVCVEGIESYEEYDIVKSLGVDIIQGFLFGRPVSASEFYKFQLSK"
     gene            complement(2618..3352)
                     /gene="pflA"
                     /locus_tag="ACGI59_001699"
     CDS             complement(2618..3352)
                     /gene="pflA"
                     /locus_tag="ACGI59_001699"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HFG9739486.1"
                     /translation="MIKGKIHSIETFGTVDGPGIRYILFFQGCPLRCKYCHNRDTWDI
                     KSGKEYTVDEIITDALKYTSFMKFSGGGITASGGESTLQPEFLSELFKKAKENDIHTC
                     LDTSGFVDIETIDPVLDNIDLVLLDLKHMVEEKSIDLTGVGMDKALKLARHLESRNIP
                     VWIRHVLVPGITDDIDNLEKLGQFVATLKNVERFELLPYHSMGIHKWESLGIDYELKD
                     VPDATKEDVQKASEIISKFGVKVYNS"
     gene            complement(3512..5743)
                     /gene="pflB"
                     /locus_tag="ACGI59_001700"
     CDS             complement(3512..5743)
                     /gene="pflB"
                     /locus_tag="ACGI59_001700"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFG9739487.1"
                     /translation="MNAWQGFKTGRWTKEINVREFIQLNYSPYEGNDSFLAGATENTK
                     KLWDEAMVLFKKERENGGTLDVDTKTVSGIAAYAPGYLDKELETIVGLQTDAPLKRAV
                     MPYGGIKMVENSCEAFGYELDPEIKDIFTKYRKTHNQGVFDVYTPEMRAARKSGIITG
                     LPDAYGRGRIIGDYRRVALYGVDALIEDKNEQKKSLEVSCMDEEVIRLREEITEQISA
                     LNELKKMAESYGFDISKPATNSKEAVQWLYFGYLGAVKDQNGAAMSLGRTSTFLDIYF
                     ERDLKAGIVTEEELQEYMDHFVMKLRMVKFLRTPDYNNLFSGDPTWVTECIGGMGIDG
                     RTLVTKNSFRMLNTLYTLGPSPEPNLTVLWSTKLPQGFKDFCSKVSIDTSSVQYENDD
                     LMRAYWGDDYGIACCVSAMRIGKQMQFFGARVNLAKTLLYAINGGVDEKSGVQVGPRF
                     EPITSEYLDYDEVMSKFEPFTDWLATLYVNTLNVIHYMHDKYSYEALEMALHDRDIFR
                     TMACGMAGLSVCADSLSAIKHAKVKTIRNEQGIAVDFEIEGDYPKYGNNDDRVDSIAV
                     ELVESFMNKIRKNKTYRNSYPTQSILTITSNVVYGKKTGNTPDGRRAGAPFAPGANPM
                     HGRDTNGALASLSSVAKLPYEHAQDGISNTFSIVPGALGKDMTERINNLSAMMDGYFA
                     QNAHHLNVNVFDRATLEDAMEHPEEYPQLTIRVSGYAVNFIKLTKEQQLDVINRTFHG
                     KMA"
     gene            6102..7097
                     /locus_tag="ACGI59_001701"
     CDS             6102..7097
                     /locus_tag="ACGI59_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HFG9739488.1"
                     /translation="MFITVVLFLVGFLLITKGADIFINCTVEIGKKTNISEIILGATI
                     VSFATTLPEFTVSLLASIDGHTTMSLGNAVGSIICNTGLALGLVVFISPFNVDKKMFF
                     SKSLLLIVSVIVLILLSLDGVITRGDSLLLIIILIFYMVNNYRSVVGKSDTKNRNIKN
                     NTIKDISTKKGKNYRGFSVLEIVKILLLFATGLIMMIIGSQILIESGVIIASFLNIPQ
                     GIVSLTIIALGTSLPEIVSSITAIRKNHHEISVGNILGANILNIVSVIAVSAIPNNIP
                     ILSQNRQLDIPFMILLLLIVIIPTLKSNKLSRIQGILMLFTYFLYISILYFMYII"
     gene            7188..8801
                     /locus_tag="ACGI59_001702"
     CDS             7188..8801
                     /locus_tag="ACGI59_001702"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFG9739489.1"
                     /translation="MNITKFEDLPISEGIKKAIAEMGFEEPSPIQAQSIPAILSGKDV
                     IGQAQTGTGKTAAFSIPILETIDPNNRSLQAVVLCPTRELAIQVSTEIRKLAKYSHGI
                     KTLPIYGGQPIDRQIKSLKSGVQVVIGTPGRTIDHINRKTLKMDNVKMIILDEADEML
                     DMGFREDIEMILSKIPEERQTTFFSATMPRGILELTKRYQKDPEHIKVVRKELTVSNT
                     KQYYIETRSSNKLEVLCRLVDVYDPKLSVVFCNTKRKADELVGDLQARGYFADALHGD
                     LKQTQRDIVMDKFRNGTIDILVATDVAARGIDVDDVECVFNYDLPQDEEYYVHRIGRT
                     GRAGREGMSFTFVFGKEMRKMKDIERYTKSKLIKHNIPTITDVEEKKVGTFFAQVKQT
                     IEEGHLTKQLQWLEGFCNDEDYAMVDIAAALVKLSLGEEMKEEIIEEKPRRERGDRKG
                     GTGAKDGMIRLFINIGRNQRVQAKDIVGAIAGEVGIPGKVVGTIDIYDKYTFVEIPKK
                     DAKTVIEKMKDIKIKGNKINIEKANKKKK"
     gene            9076..11061
                     /locus_tag="ACGI59_001703"
     CDS             9076..11061
                     /locus_tag="ACGI59_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFG9739490.1"
                     /translation="MSMTYMDPRVFLILEMVLEEDTNTLEQFSNVLGVSTRTIRNYIN
                     QINRELKGGIAKIVKNEKGTYSIRIEDESKLIEIVNFNRGKLSNFINLNSPDERINYV
                     LDILTMTNNPITIDDLAEEICIGRTTLIKDLKKADRILAEYDLELKRKPNIGMILVGE
                     EMDIRLLILDRLYENYIDVLENVNQINLVKDYDIECLRDELKKLFKKEELYITEQVLR
                     DVERYIIMSVLRNLNGYKFEKIDKRFEVIRCSDEYTLGLKLKALLEKIFNIVLNEKET
                     IFLTMPLIGRKAPSTKLALSSIKINDSVKEVVDEVTSFLLETSGIDFKEDKKLIENLE
                     YHLYFALNRMRFNIRVKNPLLQEIKERYTFPYSVAKIAAMIIEEKFDLKICDHEIGYI
                     ALHFGSYFEKNSRKVLNVNRVAVVCGTGLGTAQLIRIKIGKILGESVEVNTFSDIEAK
                     ANLDLLKYYDLVFTTVDFKTDLDVPIFRVNALFNEESIKKEIENAILLKNSINLKSIK
                     KIEKNIPFIGKLIEEDKFFILNKSTFMENLEDMLEKLVHKGIIDIEFKKKVLKREKKS
                     STALDNYIALPHSVNTNGENLYLAFGILEKPVIWDTKEIRIIILMIIPDKNVDSTDLI
                     IKSYEEVLEVGRDKKMVEELAKAKSFDEFYKIITKRR"
     gene            11066..11500
                     /locus_tag="ACGI59_001704"
     CDS             11066..11500
                     /locus_tag="ACGI59_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFG9739491.1"
                     /translation="MDPVIFIIASLITVYLLNIFLGYLQLKDFNKNYIELKRKGRVAI
                     GRKKGRISSGTIVLILINDDGVIVETRKMQGVTVLARVKSFEGLVGKNLEYIEKSDLT
                     VYNKLLKKAILDAVKQYITFEKNKCEDTNNLSTDEECRMEVV"
     gene            11529..12089
                     /locus_tag="ACGI59_001705"
     CDS             11529..12089
                     /locus_tag="ACGI59_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFG9739492.1"
                     /translation="MTQFFQVLASGADSFMNLFRAGGQQFVGFVTDIVPLLVALLVTM
                     NAIINFIGTDRVERLAKKCSANIFTRYLILPVLGTFVFANPMTLSLGRFLPEKFKPSY
                     FAAASFSCHTMNGLFPHINPGELFIYLGIANGITTLGFSTADLAVRYLLVGIVANFIK
                     GVVTDYTTKFVEKQQGIALNSDIKIG"
     gene            12153..12653
                     /locus_tag="ACGI59_001706"
                     /pseudo
     CDS             12153..12653
                     /locus_tag="ACGI59_001706"
                     /inference="COORDINATES: protein motif:HMM:NF015569.4"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbitol transporter subunit IIB"
     gene            12693..13112
                     /locus_tag="ACGI59_001707"
     CDS             12693..13112
                     /locus_tag="ACGI59_001707"
                     /inference="COORDINATES: protein motif:HMM:NF019286.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbitol transporter subunit IIB"
                     /protein_id="HFG9739493.1"
                     /translation="MAFVAVLIGIINQSGIGNWVANLLTPLIGNVGGLVVLSIICSFP
                     LLSPFLGPGAVIAQIVGVLVGTEIGKGNIPPSLALPALFAINSQAACDFIPVGLGLAE
                     AEPDTVQVGVPSVLYSRFLTGAPTVLLAWLASFGLYK"
     gene            13153..13518
                     /locus_tag="ACGI59_001708"
     CDS             13153..13518
                     /locus_tag="ACGI59_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFG9739494.1"
                     /translation="MELIYSTQINKIGDNANEFLQHNMFVTFKDNAPQELENYCYIHS
                     ENNLVKEILTDDVLSINNVDYKITSVGELVNQNLSELGHITFKFTGEKDGAIGGTLYL
                     EEKEIAPIGVGTILKIIRK"
     gene            13619..14392
                     /gene="srlD"
                     /locus_tag="ACGI59_001709"
     CDS             13619..14392
                     /gene="srlD"
                     /locus_tag="ACGI59_001709"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HFG9739495.1"
                     /translation="MRKVALVVGGGRSLGEYLSKHLAKVGYDVAIADLNEDNVKKVAE
                     EIKTEFGCKSVGYSCNAIDERQVISTVKAIENDFDRIDLLVYNAGVAVSKKITDFGYK
                     DFKFIVDVNLNGYFLFAREVSKVMIAKGIEGRIIQINSKSGRVGSKHNSGYSAGKFAG
                     VGLTQSLALDLAEHKITVNALMLGNLLDSDMFESLIPQYAVKLGIPESEVKQVYIDKV
                     PLKRGCRFIDVANMLTFYASKEAEYCTGQSINITGGQVM"
     gene            14741..16885
                     /locus_tag="ACGI59_001710"
     CDS             14741..16885
                     /locus_tag="ACGI59_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HFG9739496.1"
                     /translation="MDIIKKLSHGCTLDCHDCCKFNVYTKGNNVVKIEGDKNHPYTKG
                     FICKKGMAHLDRLNHKDRIKTPMLKVDGVWKEISFDKAIEIMAEKLTYYKEKYTSKSV
                     MHYDQYGSGSVLKYIGDIFFNFYGGVSRHKGGPCWSAGMHAQKYDFGVAKSHAIEDML
                     NSKSIFVWGKNPAYTTIHTMQIIKKAKEKGIKIVVIDPIYTKTAQIADKYVQVNPGTD
                     GALAIAMAKIIVEDKLYDEEYINSYVIGFEEYKKYLSSLELSFLIDECGVKENDIREL
                     VDLYTNKYSSINVGYGLQKYKNGGNTIRAIDALGAITGQIGFSGGGVNYANKVYPSVI
                     NSDPYNSQSYGEDREFYVSNISKFIEESLKNTSNKVNYASDELDMTSNKVNYVSDELD
                     ITSNKTDYISNELYNLSNKSIKGNIPIKMAVITKSNMLNQLPDLVELERVFSKIEFKV
                     CFDMFITDTATLCDMFIPCTNTLESEDIIYSSMTNPYITYNERAVKPAHKLMDEYYFF
                     MELAKKMGLNDYPFVEKRTYLEKVIEPLKRFDKNLDIEKLKNNYFTIHNPVAWEDKKF
                     ETPSGKYELYSESIKNLGISPTPVYISNKYKEIEDKNISFRLLTNHHADTLFSQHFMD
                     KKSIAQAYINRRMAKKVGIEDKDIVILRSKKAKINVQINIDDGVGNYIVKMYVGWWKK
                     HGNPNSLTDTGISDFGGQVTYNESMVEIIRQN"
     gene            16973..17308
                     /gene="spoIIAA"
                     /locus_tag="ACGI59_001711"
     CDS             16973..17308
                     /gene="spoIIAA"
                     /locus_tag="ACGI59_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045152 - antisigma factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0030435 - sporulation resulting in
                     formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor antagonist"
                     /protein_id="HFG9739497.1"
                     /translation="MVNYSLEHKNLYIEFMCSELDHHVANEIREEIDNLLSVNQVKNV
                     VFNFENINFMDSSGIGVIIGRYKKISNEGGRVSVINISSRVKKIFDLSGLNKIIGIYD
                     TYEEALSSL"
     gene            17322..17750
                     /gene="spoIIAB"
                     /locus_tag="ACGI59_001712"
     CDS             17322..17750
                     /gene="spoIIAB"
                     /locus_tag="ACGI59_001712"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA];
                     GO_process: GO:0042174 - negative regulation of
                     sporulation resulting in formation of a cellular spore
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor"
                     /protein_id="HFG9739498.1"
                     /translation="MNNIMEVKFSAISENESLARVIVASFAAKLDPTVDELVDIKTAV
                     SEAVTNAIIHGYEEDSSKFVFLRCEIEGNTIKIIVEDEGYGIENVEKAMEPLYTSKPE
                     LDRSGMGFTVMKSFMDDVEVSSVKDNGTRIEMTKKINVPK"
     gene            17773..18537
                     /gene="sigF"
                     /locus_tag="ACGI59_001713"
     CDS             17773..18537
                     /gene="sigF"
                     /locus_tag="ACGI59_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigF"
                     /protein_id="HFG9739499.1"
                     /translation="MEVTVAREEKKPLLSHEETLELIEKVQNGDEEAKEILISSNLGL
                     VRSVVSRFLNIGYDREDLFQLGSIGLIKSIYKFDPKFNVKFSTYAVPMILGEIKRYLR
                     DDGMIKVSRSLKQIAIKAKMESEALTKKLGREPSIEELAKAIDVEKEDLVMAMEANFN
                     VEYLQGVIHEEEGSPICLIDKISMKGESEEEKVVDNILLKDILGRLDKRERQIIVLRY
                     FEDMTQSEIGEMLNISQVQVSRIEKKVLSKLKEYIS"
     gene            18817..19254
                     /gene="spoVAC"
                     /locus_tag="ACGI59_001714"
     CDS             18817..19254
                     /gene="spoVAC"
                     /locus_tag="ACGI59_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AC"
                     /protein_id="HFG9739500.1"
                     /translation="MDKNYKKYVDQISPKPTYLKNYTLAFIVGGIICMIGQAINDLYM
                     KVGGLDKLGASSATSITLIFIGAFLTGLGVYDLIGKRAGAGSIIPITGFANSIVSPAM
                     EYKREGYVLGVGANLFKIAGPVLVYGIGSSILCGIIYYIFKMF"
     gene            19421..20434
                     /gene="spoVAD"
                     /locus_tag="ACGI59_001715"
     CDS             19421..20434
                     /gene="spoVAD"
                     /locus_tag="ACGI59_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AD"
                     /protein_id="HFG9739501.1"
                     /translation="MKNKRIGKRTVKLENKPTIISTGTIVGPKEGEGPLKDYFDMIMT
                     DDLYGEKTWELAESKMVETASQQAIQKAGKKLSDVNYMLGGDLINQIVPASFAARELA
                     IPFLGIYGACSTMAEGLCIGSMLIDGGFADLVLSGTSSHYCTAERQFRFPLELGNQKP
                     MTAQWTVTGAGSVLLAPNGDGPKVKYVTVGKVIDEGIDDGNNMGPAMAPAAIDTIYSY
                     FEDTNDDPNSFDIIATGDLGTLGKQIAEDFLKEKGVDISKVYTDCGIEMFNLKEQDVH
                     CGGSGCGCSATVFAGYIYDKLRKKEFNKVMLVSTGALLSPTSTLQKQTIPCVAHAVVI
                     VNE"
     gene            20448..20810
                     /gene="spoVAE"
                     /locus_tag="ACGI59_001716"
     CDS             20448..20810
                     /gene="spoVAE"
                     /locus_tag="ACGI59_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AE"
                     /protein_id="HFG9739502.1"
                     /translation="MLMDYVRVFIVGGIICLIAQVMMDYFKMQTPYVMVTYVTTGVVL
                     TFLGLYEPLVKFAAAGATVPIIGFGYSLATGVIKSIQSDGFLGIFLGGTTATAGGIAA
                     AIFFGYIMSVVFTPKAKP"
     gene            20993..22009
                     /locus_tag="ACGI59_001717"
     CDS             20993..22009
                     /locus_tag="ACGI59_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFG9739503.1"
                     /translation="MVTIKDIARNLGISYSTVSRCLNNNPNVSEKTKKKVVEEANRLG
                     FHFNVNARNLAKKETNRIGVIFSNNFNHQDTRKFFSDIMDSSINSIETNKYDFIIQPN
                     NNISGDSNVYKMVNGQMVDGLVIVSQSIKKEEYDFLKDNNFPHVFIYFKPSFIGEVDN
                     FFWDDNVYGGYIATKHLIDHGHKDIITVTSDDKSSKMHEDRTKGYLNAMEEANLKTEV
                     IKSKMDFESQVEFLEKYIDKIKKASAIFVQQDVPAISIIQELKTTYGINVPEDISIIG
                     YNNIELISYFRSHLSTIDDPREQVIKNGVDSLVNIINKKSTEHIPRKLYPRLIIRNSV
                     KRIK"
     gene            22384..23226
                     /locus_tag="ACGI59_001718"
     CDS             22384..23226
                     /locus_tag="ACGI59_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3100 domain-containing protein"
                     /protein_id="HFG9739504.1"
                     /translation="MKEKAGIRNAIIAAFIITILAELIGPISFKVMGINVTLLSILWA
                     IFIGMAVSPHLLGRVIPALKKFIGDNEINVSPYLLSLTLYPLGIMFGINAGPKVGILL
                     QAGPALLLQEFGHMGTMLIALPVGVALGLGRSALGGTFSLCRDTALGIIGDKYGLNSP
                     EGIGTLGTYISGSIFGTLLFSFLAPVGVLLGFHPYALAMASGMGSGSMMGAATASLMN
                     TVPQMSEQILAYSATSGLITAVTGIYIELFLSLPVANYYYSKVAPVIERFRGRKTRRT
                     SNKL"
     gene            23241..23705
                     /locus_tag="ACGI59_001719"
     CDS             23241..23705
                     /locus_tag="ACGI59_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9739505.1"
                     /translation="MEKTFKTIMEQIKIVLFIYIIICFGQMIALKTQAQDLLIGSVSG
                     FLLVIIAIIIKNFVKKPNLPGFAWATLVAFVLSLPISPVGDIIATNVGKINFMSTVTP
                     LLAFAGISVGNQLDILKTLSWKLVVISFIVMTSTYFGSAFISQTVLRLNGML"
     gene            23719..24864
                     /locus_tag="ACGI59_001720"
     CDS             23719..24864
                     /locus_tag="ACGI59_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFG9739506.1"
                     /translation="MNELKAKIKEEIKLLSEEKKTSFEKVSDYLFSNPELAFEEYKSQ
                     KALCDLLEENEFNVTKGVGGLETSFEAVYSNGTNGKTVAFLAEYDALPGMGHACGHNI
                     IGTSSVGAGIILKEIMKKHNIEGTVKVFGTPAEERVGGKITMIKEGVFNNVDAALILH
                     PSDASMPDDISFAQVNLKFDFTGKASHAAAFPWEGKSALSGVIALFNSVNSMRLHLKD
                     YARVHGIITDGGSIHNIIPEKSTAIFNVRALSIEYLNEICEMLKNCAKGAAISTGTNV
                     EIVQLDEIYKEIKNDSELVNIVRQNFEVLGEDYVERDLSQGIGSTDTGNLTHEIPAIQ
                     AYIKLKENTATHTDEFAVAAGGEEGRVALIKAIKVLAMCGVDILYSK"
     gene            24908..25843
                     /locus_tag="ACGI59_001721"
     CDS             24908..25843
                     /locus_tag="ACGI59_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1177 domain-containing protein"
                     /protein_id="HFG9739507.1"
                     /translation="MILKQVIEVYDILDKANANGEEVKTYLQGYGEVDVTVKELSSSK
                     GSTDLVKLTIPGKNGKLKGGDAPTLGILGRLGGIGARPEVIGFVSDGDGALVAIAVAA
                     KLLDMQRKGDILDGDVVISTHICPDAPTKEHYPTPFMDSPIDMMTMNENEVDSSCDAI
                     LSIDTTKGNRVINTRGFAISPTVKEGYILKTSDNLLDIMQTVTGKSPFVFPLSIQDIT
                     PYGNNLYHLNSILQPAVATDAPIVGVAITTEMPVAGCATGATNFSDLDSAGRFAIEVA
                     KLFGRNKCDFYDKEEWEMLIKRYGKLNRFQTFGIQ"
     gene            complement(26117..28726)
                     /locus_tag="ACGI59_001722"
     CDS             complement(26117..28726)
                     /locus_tag="ACGI59_001722"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HFG9739508.1"
                     /translation="MNNDNDKNGNKIRRKKVSSSGNTNKPINRTSANKTRSTKNKKKS
                     KKSDKFKKLRVFGIVFLVLLVVGTAGTAGLVFASLRDVTPVTEAVLDKQTNQTTTIKY
                     ANGKTLSTAPSVNKKTPVPLDKISPYLQHAVIAIEDERFYEHKGVDIKGLFRSVLKTL
                     TGTKQGGSTIPMQVSKMLLTTEQQTIPRKIKDIYYAHEMSKTVSKDKILETYLNNFFV
                     GRGLAGAEAGARGYFDKSAADLTLAESALLAGSTKNPSRFSAYKTSKLEGNETKEDLE
                     NKLLFFVNTTDDDLDDPTQVDFDMIEKIKSWELISNDTYRQLKAGTLVVRKAVSNPEA
                     KKRQEIVLKKMLELKYIKQSEYDEAIKAKIEIKLPQSSDKVSSSVEDLIESEVINALM
                     EQGHTNDEAQNLFYNGGLIVNTTIDPKMQDALEEEFDRNSNFPGHMVGPDGVSQPQAA
                     MVILDYKNGEIRALAGGRNISGRKTLNRATNPHQPGSSIKPLAIYTPAIDTLKITQAT
                     ALSDSRGGYKFEENNKWNPRTTTAGHGSMSLRKALAKSSNTIAVKTAEMLGDSYDECV
                     DIMMDYLKNFGITTLKNNHSGSSEASDRKFPSLTLGGMANGITPLQMAAAYGTLANHG
                     IYVEPSIFTTITTFDGQLLVKNAPEEHKVVDPEVAYVVTDMLESVITEGTGGVATLPK
                     GMPVAGKTGTTNSAYDAWFVGYTPYYVGATYIGDDAGRKDDSGNTIKRREVPHGSTST
                     AKLWEKIMEKIHANLTVTEFEVPKNVYFTKINLEDGGKQSSGSKAAFIEGTAPTKVSS
                     QPSSEDTKKPDNNQPEENNNNNNNTGDNGGGSTPPDNGGNNGGGSTTPPDGGGNNGGG
                     STTPPDGGGTPAT"
BASE COUNT        10898 a         3435 c         5004 g         9513 t
ORIGIN      
        1 ataattaaaa taaaaaatat tattttatat ttataaacat tgacattcta attttgtagt
       61 gtaatcgtat tttttattaa ttcttacaaa atcaatgaaa ataagtatat ccattttaaa
      121 gtacataaat ttaaaaatcc agaaaaatat acttaaatta tgtaagtaaa agaggggttg
      181 aatcagttga aaatgtaata ttagtaaata gtacatcaat cctatatacc ctgtaagcaa
      241 ctctattttc atgtactaaa aatgtaccta ttttattaac tgaaaagaat aaattaaatg
      301 taagtacata agaagataga aaaaattaaa aacaaaaaat atttatataa ataaatataa
      361 aaaatgatat aatccctatt aaaaaattta tagatgatga agaaatatca aaatcttata
      421 ttataggaag agacacttct ttgccaaaat ctatagaaaa gcaataccaa atccaaagag
      481 aataggcgaa gaaaaaaata aaataaattc aagagtaata gaaaagtttt atagaataaa
      541 gaactgataa ctctttatat aacgaaaaat agaattttaa gagaaaatga tttaataaat
      601 gatttaacat ggtgttttag caggtaaaaa taaatcacca gtaatgatta tagaaaaatt
      661 actttatgaa tctcaaaaaa cttagttaag agtaagagat ttgataagat aactaaagtt
      721 ggggaaatgt gtttgatgag acaaaaaatt agtaaaatag tataatctaa ataaggatat
      781 tttgttgatt aaactacact aatagtaata ttgtaggggg aaaagatgag agaagaatcg
      841 aactctttaa gtttagacca tatagaaatt ttctttgaac ttttatctca aagtacagaa
      901 gattatatat ttttttggga tataaatagg aataaattta aaatttcttc agcaattttt
      961 gatgaattta acttaagtaa agagatagaa tctgatttgg tcaattgttg gagtaaaatt
     1021 gtttatccgg atgatgttca aatatggaaa gatgatatac aagaattact acatggaaaa
     1081 aaaggtgagc acaatttaga atataggttg attaataaat ataaagaaat agtatggatt
     1141 tcttgtagag gtaaagttta tgtgtcggat gacccaaaaa ctatatttct agtcgggaga
     1201 ataaagaata ttggtgaaaa aaataaattt gattctatca caggaacgtg gaatagagaa
     1261 caatttgaac atagaatgaa ttatcttata aaagagaaaa tttataaaaa tggtgctatg
     1321 tttattatgg atattgataa ttttaaaaat ataaatgaaa aatatggaca ttcttatggt
     1381 gacaaagtat tgcgtgcaat tgctactgaa gtattagaat atcttccaaa agatgtaaga
     1441 ttgtataggt tagatggaga tgaatttgca tttttttacc ctatgtgtac taaagaaact
     1501 atagagaaaa tatatgaaaa gatacagatg tatacaaata ctcagcatga aattgaatca
     1561 aataagtact attgcacagt gactgcaggc gttgctatgt atccagaaga tggagataat
     1621 tatttagatt tatttaaaca tgctgatatt gcattggata tagcaaaaat tagtggtaaa
     1681 aatagaatta agtttttttc acaagagctc tatgaaaata aactaaaggt aatttcaatg
     1741 caacaaaaac taagagagtg cgttgaaaat aattttaatg attttgagtt gttttttcaa
     1801 ccccaggtaa atgctgttac aaaagaggta ataggggcag aggtactttt aagatggcat
     1861 tcgagtactt atggagaagt atctcctgtt gaatttatac caatactaga acagagtaat
     1921 ttaattatac cagttgggaa gtggattata aaagaagctg taaaacagtg taaagaatgg
     1981 cataaaataa atccagactt taaaatatct gtaaatgtat cgtatattca gttaaaggaa
     2041 gattttttta gagattttat agtagaatgt ttagtagagt atcaattaag acctgaattt
     2101 ttaattttgg aattaacaga gaattgttgg ataccagata taaatttact aaatgataaa
     2161 tttattagtt taaagggtat aggagtatat attgcaatag atgactttgg tacaggatat
     2221 tcatctttaa attaccttaa agaactgtct gttaatataa taaaaattga aagaagtttt
     2281 gttaagaata ttacatataa tagttatgaa tatacttttc ttgaatatat tattaaatta
     2341 gctcatatta taaatttaaa agtttgtgtt gagggaatag agtcttatga agagtatgat
     2401 atagtaaaaa gcttaggtgt agatattatt cagggttttt tatttggaag acctgtaagt
     2461 gcctctgaat tttataaatt tcagctatca aagtgatgta tataaaatga gatatatagt
     2521 atataaaaat agggcaaagt ggaagttttg aagtttatta aaaatttaca ctttacccta
     2581 ttttgttatg tcttctataa aggattaata tactacacta actattatat actttcactc
     2641 caaatttaga gattatttca ctagcttttt ggacatcttc cttagtggca tcaggaacat
     2701 cttttagttc ataatcgata cctaaacttt cccatttgtg tattcccata gaatggtatg
     2761 gtagcaattc aaatctttct acatttttca atgtagctac aaattgacct agtttttcta
     2821 aattatctat atcatcagta atgccaggaa ctaaaacatg tcttatccat acaggaatat
     2881 ttcttgattc taagtgtcta gcaagtttta atgccttatc cataccaaca ccagtcaaat
     2941 caatagattt ttcctcaacc atatgtttta aatcaagtaa aaccaaatca atattatcta
     3001 aaactgggtc tatagtttct atatctacaa atccagatgt atccaagcaa gtgtgtatat
     3061 cattttcttt tgctttttta aataattcac ttaaaaattc tggttgaaga gtggattcac
     3121 caccagaggc agttatacct ccaccagaaa acttcataaa agaagtatac ttaagagcat
     3181 ccgttattat ttcatcaact gtgtattctt ttcctgattt aatatcccat gtatccctgt
     3241 tatgacagta tttgcatcta agaggacacc cctgaaaaaa taatatatat cttattcctg
     3301 gtccatctac agtaccaaac gtttctatag aatgtatctt tcccttaatc atagagttca
     3361 cctcacaata tattttaata accttatcaa tagttataat atttctctaa aaaaggccat
     3421 cttataccag aaataattca aatataagat agccttttat ttttattttt tacacagtag
     3481 taatcttagt taaagtataa aaatttaaag actaagccat tttaccgtgg aatgttctgt
     3541 ttataacatc taattgttgc tctttagtta attttatgaa gttaactgca tatccagata
     3601 ctcttatagt taattgtgga tattcttctg gatgttccat agcatcttct aaagtagctc
     3661 tatcaaatac atttacattt aagtgatgag cattttgagc gaagtatcca tccatcattg
     3721 cagaaagatt gtttattctt tcagtcatat cttttcctaa agcaccaggt actatagaga
     3781 aagtattaga tataccatct tgagcatgtt catatggtaa tttagcaact gaagataatg
     3841 aagctaaagc accattagta tctctaccat gcattgggtt agcaccagga gcaaatggag
     3901 caccagctct tctaccatct ggtgtattac cagttttctt accataaaca acatttgaag
     3961 ttattgtaag tatagattga gttggataag aatttctata agttttattc tttcttattt
     4021 tattcataaa gctttcaact aactctacag ctatactatc aactctatca tcattatttc
     4081 cgtattttgg ataatctcct tctatttcaa aatcaacagc tattccttgt tcatttctaa
     4141 tagttttaac cttagcatgt tttatagcag ataatgagtc tgcacaaact gataacccag
     4201 ccataccaca agccatagtt ctaaatatat ctctatcatg taatgccatt tctaatgctt
     4261 cataagaata tttatcatgc atataatgta ttacatttaa agtatttaca tataatgttg
     4321 ctaaccaatc agtgaatggt tcaaatttac tcataacttc atcataatct aagtactcag
     4381 aagttatagg ttcaaatctt ggtccaactt gaacaccaga tttctcatct acaccaccat
     4441 ttatagcgta taataaagtt ttagctaagt taactctagc tccaaagaac tgcatttgtt
     4501 taccaattct cattgcagat acacaacaag ctataccata gtcatctccc cagtatgctc
     4561 tcattaaatc atcattttca tactgtactg aacttgtatc tatagatact ttagaacaga
     4621 aatctttgaa tccttgaggt aatttagttg accatagaac tgttaagttt ggttctggag
     4681 aaggtcctaa tgtatataga gtatttaaca ttctaaatga gtttttagta actaatgttc
     4741 taccatctat tcccatacct cctatacact cagttaccca agttgggtct ccagagaata
     4801 ggttattgta atcaggagtt cttaggaact taaccattct taatttcata acaaaatggt
     4861 ccatatactc ttgtaattct tcttctgtta cgattccagc ttttaaatct ctttcaaagt
     4921 atatatctaa gaaagtagat gttctaccta aagacattgc agcaccattt tggtctttaa
     4981 cagcacctaa ataaccaaag tataaccatt gtacagcttc ttttgagtta gttgctggtt
     5041 ttgatatatc aaatccataa gattcagcca tttttttaag ttcatttaaa gctgatattt
     5101 gctcagttat ttcttctctt aatctgataa cttcctcatc catgcaagaa acttctaatg
     5161 atttcttttg ctcattttta tcttctatta aagcatcaac accgtataga gcaactcttc
     5221 tgtagtcacc aattattcta cctctaccat aagcatctgg aagacctgtt attataccag
     5281 attttctagc agctctcatt tctggagtat aaacatcaaa aactccttgg ttgtgtgttt
     5341 ttctgtattt agtaaatata tcttttattt ctgggtctaa ttcatatcca aaagcttcac
     5401 atgaattttc aaccatcttt ataccaccat atggcatgac agctcttttt aaaggggcat
     5461 ccgtttgtaa cccaactatt gtttctaatt ctttatctaa atatcctgga gcatatgcag
     5521 ctattccaga aacagttttt gtatcaacat ctaaagtacc accattttct ctttcttttt
     5581 taaataggac cattgcttcg tcccataatt ttttagtatt ttcagtggca ccagctaaga
     5641 aagagtcatt tccctcatat ggagaatagt ttaattgtat aaactctcta acatttattt
     5701 ctttagtcca tcttcctgtt ttaaatcctt gccatgcatt cattaagtat ttcctcctta
     5761 gatttaaatg ataaataata ctattaaaat taatttttat atacttaatt tattttatat
     5821 atttttttaa aaatatattt tctaatataa taatagcata ttattcgtat cctgtataca
     5881 taaaaaataa aaaattgtcg ctttaaagta taaaacagat tatatgttaa taatgtggtg
     5941 aaattatacc catatataaa taaaataaaa caaataaata gctaattaag gagaaaatat
     6001 tttttatttt ttgtttagga taaataaatt ttattatgga aaaaagaaaa agcatacatc
     6061 ttcacaaact gaatatttat ttaatagggg gaggtgatag attgtttatc acagtggtat
     6121 tatttttagt aggtttccta ttaattacta aaggcgcaga tatctttata aattgtactg
     6181 tagagatagg taaaaaaacg aatatatcag aaattatact tggagctact atagttagtt
     6241 ttgcaacaac attacctgag tttacagtgt ctctacttgc ttctattgat ggtcatacta
     6301 ctatgagttt aggaaatgca gttggttcta taatatgtaa tacagggtta gcattagggt
     6361 tggttgtttt tataagtcca tttaatgtag ataaaaaaat gtttttttct aagtctttac
     6421 tattaatagt atccgttata gttttaattt tattgagttt agatggagtt ataacaagag
     6481 gtgattcatt attacttata ataatattaa tcttttatat ggttaataat tatagaagtg
     6541 tcgttggaaa atctgatact aaaaatagaa atattaaaaa taacactatt aaagacatat
     6601 ctactaaaaa agggaaaaat tatagaggtt tttccgtatt agaaattgta aaaatactat
     6661 tactttttgc tacaggattg ataatgatga taataggttc acaaatcctt atagagagtg
     6721 gagtaataat agctagtttt ttaaatatac ctcaaggtat agtaagttta actataatag
     6781 cacttggaac ttctctgcca gagatagttt cgtcgattac tgctataaga aaaaatcatc
     6841 atgaaatatc agtaggaaat attttgggtg caaacatatt gaatatagta tcagtaatag
     6901 ctgtatcagc aataccaaat aacataccaa tactatctca aaatagacaa ttggatatac
     6961 cattcatgat attattatta ttaatagtaa taataccaac acttaaatct aataaactta
     7021 gtagaataca aggtatattg atgttattca catattttct gtacatatca attttgtatt
     7081 tcatgtatat tatttaacta tactgtttaa aatattaatt gcagaatatc ctaaaaaagt
     7141 atataatgga taggttaaga ttaattgata acggagagga ataatatatg aatataacaa
     7201 agtttgaaga tttaccaata agtgagggaa taaaaaaagc tatagctgaa atggggtttg
     7261 aagaaccatc tcctatacaa gcacagtcta tacctgcgat tttgtcaggt aaagacgtta
     7321 taggacaagc tcaaactggt acgggaaaaa cagctgcttt tagtatccct atactagaaa
     7381 caatagaccc aaataataga agtttacaag ctgtagtgct ttgtccaaca agagaactgg
     7441 caatacaagt atcaacagaa ataagaaagt tagctaagta ttcgcacgga ataaagacat
     7501 tacctatata tggtggtcag ccaatagata gacaaataaa atctctaaag agtggtgtgc
     7561 aagttgtaat aggtacacca ggacgtacaa tagaccatat caatcgtaaa acattaaaga
     7621 tggataatgt aaaaatgatt atattagatg aagcagatga aatgctagat atgggattta
     7681 gagaagatat agaaatgatt ttaagtaaga ttccagaaga aagacaaaca actttctttt
     7741 cagctactat gccaagagga attttggaat taactaagag atatcaaaaa gacccagagc
     7801 atataaaagt tgttagaaaa gaacttacag tttcaaatac aaagcaatac tatatagaaa
     7861 ctaggtcatc aaataaatta gaagtattat gtagattagt agatgtatat gaccctaaac
     7921 tatctgttgt tttctgtaat actaagagaa aagcagacga attggtagga gatttacaag
     7981 caagaggata ttttgcagat gcattacatg gtgacttaaa acagactcaa agagatatag
     8041 taatggataa gtttagaaat ggtacaatag atatacttgt tgctactgat gttgcagcta
     8101 gaggtataga tgttgatgat gttgaatgtg tattcaacta tgacttacca caagatgaag
     8161 aatattacgt tcatagaatt ggtagaactg gtcgtgctgg tagagaaggt atgtcattta
     8221 cttttgtatt tggaaaagaa atgagaaaga tgaaagatat agaaagatat actaaatcta
     8281 aactaataaa gcataatata ccaacaataa ctgatgttga agagaaaaaa gtaggtactt
     8341 tctttgcaca agttaaacaa actatagaag aaggtcactt aactaaacaa ttacaatggt
     8401 tagaaggttt ctgtaatgat gaagattatg caatggttga tatagctgct gcacttgtaa
     8461 aactatctct aggtgaagaa atgaaagaag aaataataga agaaaaacct agaagagagc
     8521 gtggagatag aaaaggtggt actggagcta aagatggtat gattagatta tttatcaata
     8581 ttggtagaaa tcaaagagta caagcaaaag atatagttgg tgccatagct ggagaagttg
     8641 gaataccagg taaagtagta ggtactatag atatatatga taagtatact tttgttgaaa
     8701 tacctaaaaa agatgcaaaa acagtaattg aaaagatgaa agatattaag ataaaaggta
     8761 ataagattaa tatagaaaaa gcaaataaga agaaaaaata acgtatagta tacaaaacta
     8821 taattagaaa atatataaaa taatatattg gatatttaaa aagcctgatg tgttttcaaa
     8881 acagatagta gataatctgt aagagttaac agcataaggc ttttcttgtt ttatatgatg
     8941 ttataatttt ccagattgtt gtgaaaaatg tatattagtt cctttttttc aaatctgatt
     9001 tgaaaaagag aatattaatt atctatatag tttagattat aataaaaaat gtaagataac
     9061 aaaggggaga aaaagatgtc aatgacttat atggacccta gggttttttt gattttagaa
     9121 atggtgttag aagaagacac aaatactcta gaacaatttt caaatgtttt gggtgttagt
     9181 actaggacta tcagaaacta tataaaccag attaatcgag aattaaaagg tggtattgct
     9241 aaaatagtta aaaatgaaaa aggtacatat agtataagaa ttgaggatga aagtaaatta
     9301 attgagattg taaattttaa tagaggaaag ctatcaaatt ttataaattt aaattcacct
     9361 gatgaaagaa taaattatgt tcttgatatt ttaactatga ccaacaatcc aattaccata
     9421 gatgatttgg ctgaagagat atgtatagga agaacaacat taataaagga tttaaaaaaa
     9481 gcagacagaa ttttagctga atatgattta gaattaaaaa ggaagccaaa tataggtatg
     9541 attttagttg gtgaagaaat ggatataaga ttattaatcc ttgatagatt gtatgaaaat
     9601 tatattgatg tcttggaaaa cgttaatcag ataaatttag ttaaagatta tgatattgaa
     9661 tgtcttagag atgaattaaa aaagttattt aaaaaagaag aactctacat aacagaacag
     9721 gtcttgagag atgttgaaag atatataatc atgtctgtct taagaaatct aaatgggtac
     9781 aaatttgaaa agatagataa gagatttgaa gtgataagat gttcagatga atatacttta
     9841 ggattaaagc tcaaagcttt attggaaaag atatttaata tagttttaaa tgaaaaagaa
     9901 accatatttt tgacaatgcc attaataggt agaaaagctc ctagtacaaa attagcactt
     9961 agcagtataa agataaatga tagcgtaaag gaagttgtag atgaagtaac tagtttttta
    10021 ttagagacct cagggattga ttttaaagaa gataaaaagc ttatagaaaa tttggaatat
    10081 cacttgtatt ttgcacttaa tagaatgaga ttcaacataa gggtaaaaaa tcctctttta
    10141 caagaaataa aggaaagata tacatttcca tatagtgttg caaagatagc tgcaatgata
    10201 attgaggaaa agtttgactt gaaaatatgt gaccacgaaa taggatatat agcattacat
    10261 tttggaagtt attttgaaaa aaattcaaga aaagttctaa atgtaaatag ggtagctgtt
    10321 gtttgtggaa caggtcttgg gactgcacaa cttattagaa taaagatagg aaaaatactt
    10381 ggagaaagtg tagaagtaaa tactttttct gatatagaag ctaaagctaa tttagattta
    10441 ttaaagtatt atgatttagt atttacaaca gtagatttca aaacagactt agatgttcca
    10501 atttttagag taaatgcgtt atttaatgaa gaaagtataa agaaagagat agaaaatgca
    10561 atacttttga aaaatagtat aaatcttaag tctataaaaa agattgaaaa aaatatccct
    10621 tttataggta aattaattga agaggataag ttctttatat tgaataagag tacgtttatg
    10681 gaaaatttag aagatatgtt agaaaaatta gttcataaag gaattattga tatagagttc
    10741 aaaaaaaagg tattaaaaag agaaaagaaa tcttcaacag cattagataa ttatatagca
    10801 ttgcctcact cagttaatac taatggggaa aatctttatc tggcatttgg aatattagaa
    10861 aaaccagtga tttgggatac aaaggaaatt agaataataa tattgatgat aattccagat
    10921 aagaatgtag atagtacaga cctgataata aaaagttatg aagaagtact tgaagtaggg
    10981 agagataaaa agatggtaga agaacttgct aaggcaaaat catttgatga attttataaa
    11041 ataattacca agaggaggta ataatatgga cccagtaatt tttattatag catcacttat
    11101 tacagtgtat ttattaaata tttttcttgg atacttacag cttaaagatt ttaataaaaa
    11161 ttatattgaa ctaaaaagaa aaggaagagt ggcaattggc agaaaaaaag gaagaatttc
    11221 atcgggtaca attgtgttga tacttataaa tgatgatggt gtgattgtcg agactagaaa
    11281 aatgcaaggt gttacagtat tggcaagagt aaagtcattt gaaggtttgg taggaaagaa
    11341 cttagagtat atagaaaaat cagatttaac tgtttacaat aaactactta aaaaagcaat
    11401 actagatgca gtcaaacagt acataacatt tgagaaaaat aaatgtgaag atacaaataa
    11461 tctgagtact gatgaagagt gtaggatgga agtagtttaa aaaaatatat ttataaggag
    11521 gagttaatat gacgcaattt tttcaagtat tagcaagtgg tgcagatagt tttatgaatt
    11581 tatttagggc aggtgggcaa caatttgtag gttttgtaac agatatagta ccacttttag
    11641 tagcattatt agttacaatg aatgcaatta tcaactttat tggtacagat agagtcgaga
    11701 gacttgcaaa aaaatgttct gcaaatatat ttactagata tttgatatta ccagtccttg
    11761 gtacatttgt atttgcaaac ccaatgacac tatcattagg aagattcttg ccagaaaagt
    11821 ttaaacctag ttactttgca gcagcaagct tttcatgcca tacaatgaat ggattattcc
    11881 cacatatcaa tccaggtgaa ctatttatat atctaggaat tgcaaatggt ataacaactt
    11941 tgggattctc aacagcagat ttagcagtta gatatttgtt agtgggtata gttgcaaact
    12001 ttattaaagg tgtagttact gactatacaa caaaatttgt agagaaacaa caaggaatag
    12061 cattaaattc agatataaag attggataat tactttaagg tataaataaa tttttcaaat
    12121 aaaaagatag tgtatagagg aggataaatt atatggcatt taacaaagtt aaaatagtaa
    12181 aaggttctgg aggatggggt ggaccattga taatagaacc aacagaaaag aagaataagg
    12241 tagtatatgt gactggggga gcaaagccag aaactgcagt aaaaatagct gaacttacag
    12301 gatgtgagtt aatagatgga ttttcacagg gtgttaaaga tgatgaaatc gcatgtgtaa
    12361 taataaattg tggtggaaca ttgagatgtg gtatatatcc tcaaaagaaa ataccgacta
    12421 taaatataat gaaaacagga aaaagtggac cattagcaat gtttataaaa gaagatatat
    12481 atgtttcagg ggtaaaacca acaaatgtag agcagttata attgaggtag taggagggaa
    12541 gaaatatggc ttatgatact aaaaagaaga taagtgagca gagtcaagga attgttgcaa
    12601 aaataggtat agcactagga aatgtgacaa gtactctttt tcaagctggt agggatacta
    12661 tagatacatt aatacatact atactgcctt ttatggcttt cgttgcagtc ctaataggta
    12721 taataaatca atcaggtata ggaaattggg tagccaattt acttactcca ttaatcggaa
    12781 atgttggagg tttagttgtc ttatcaataa tatgttcatt tccgttgttg tctccgtttc
    12841 ttggaccagg agctgttata gctcagatag tgggagtatt ggttggtaca gaaataggaa
    12901 aaggaaatat tccaccaagt ttagcattac cagcattgtt tgcaataaac tcacaagcag
    12961 cttgtgactt tataccagta ggtcttggtc ttgcagaagc tgaacctgat acagttcaag
    13021 taggagttcc gtcagtttta tattctagat ttttaacagg tgcaccaact gttctattag
    13081 catggttagc aagctttggg ttatataaat agaagaatac tttataaata attagagaga
    13141 ggttcagttg atatggaatt aatatacagt actcaaataa ataaaatagg agataatgca
    13201 aatgaatttt tacaacataa tatgtttgta actttcaaag ataatgctcc tcaagagtta
    13261 gaaaattact gttatataca ttctgaaaat aatttagtaa aagaaatact tacggatgat
    13321 gtgctatcaa taaataatgt tgattacaaa attacttctg ttggtgagtt agtaaaccaa
    13381 aatctttcag aattaggtca tataacattt aagtttacag gtgaaaaaga tggagcaatt
    13441 ggaggaacct tatatcttga agaaaaagaa atagcaccta ttggcgtagg tactatacta
    13501 aaaataatta gaaaataaaa tttaaaacga atctaatcat attataagta aattatagat
    13561 aaaagtcaat ttaactaatc aagttaactt ttaatgtgat aaaaaagggg aaatgaaaat
    13621 gagaaaagtg gctttagtcg ttggaggagg aagaagttta ggtgaatatt tatctaaaca
    13681 tttagctaaa gtaggttatg atgttgctat tgcagattta aatgaagaca acgtaaaaaa
    13741 agtagctgaa gaaataaaaa ctgaatttgg ttgtaaatct gtaggatata gttgtaatgc
    13801 catagatgaa agacaggtta taagtactgt caaagctatt gaaaatgatt ttgatagaat
    13861 agatttatta gtgtacaatg caggagtggc tgtaagtaaa aaaataactg attttggata
    13921 caaagatttt aaatttattg tagacgtaaa tttaaatggg tactttctat ttgcaagaga
    13981 agtatctaaa gtcatgatag caaaaggtat tgagggtaga ataatacaga taaattcaaa
    14041 atcaggtaga gtgggaagca agcataattc aggatattca gcaggtaaat ttgctggagt
    14101 aggacttact caaagtttag cgctagattt ggcagaacat aagataactg taaatgcact
    14161 gatgctcggt aatttacttg attctgatat gtttgaaagt ttaattcctc aatatgcagt
    14221 aaaattggga atacctgaat cagaagtcaa gcaagtttat atagataaag tacctttaaa
    14281 aagagggtgt agatttatag atgttgcaaa tatgcttaca ttttatgctt caaaggaagc
    14341 tgaatactgc actggtcaat ctataaatat tactggtgga caagttatgt aaggttttta
    14401 tggagtgtct caagatgaac tctttagttc atgaggcact tttttgtata aaattaaata
    14461 tattttcagc atttaaacaa tgaaaaagag gtttatcttt attttgagat agcctcattt
    14521 ttgtaattaa aaaacatagt gtttaagtaa acatataaga ttgttagttt tttaaaagag
    14581 aattttttaa taatattata tttatgaagt atttttaaac ttattttgat aaaatatagc
    14641 tatgttttat atactgtagt tttgaacata gaaagtttaa taaattatag tatatatgtt
    14701 aatttttaat aaggtaagaa agtagaaaga aggatatttt atggatataa taaaaaaatt
    14761 aagtcatggg tgtacattag attgtcatga ctgttgtaaa ttcaatgttt atactaaagg
    14821 aaataatgta gtaaaaattg aaggagataa aaatcatcct tatacaaaag gttttatatg
    14881 caaaaaaggg atggcccatt tagatagatt aaatcataaa gatagaataa agactcctat
    14941 gcttaaagtt gatggagtat ggaaagagat ttcttttgat aaagctatag aaataatggc
    15001 agaaaagctt acatactata aagaaaagta tacatcaaag tctgtcatgc attatgacca
    15061 atatggaagt gggtctgtgc taaaatacat aggagatata ttttttaatt tttatggagg
    15121 tgttagcaga cataaaggtg gtccctgttg gagtgcaggg atgcatgctc aaaaatatga
    15181 ttttggagta gctaagagtc atgctataga agatatgtta aatagtaaaa gtatatttgt
    15241 gtggggaaaa aatccagcat atactacaat tcatactatg caaataatta aaaaagctaa
    15301 agaaaaaggg ataaagatag tagtgattga ccccatatat acaaaaacag ctcaaatagc
    15361 agataagtat gtacaagtaa atcctggaac agatggagct ttagctatag ctatggcaaa
    15421 aattatagta gaagataaat tatatgatga agaatatata aactcgtatg ttattggttt
    15481 tgaagaatat aaaaaatatt tatcttcatt ggaattaagt tttttaattg atgaatgtgg
    15541 tgttaaagag aatgatataa gagagttagt ggatttatat actaacaaat attcaagtat
    15601 aaatgttgga tatgggcttc aaaaatataa aaatggtgga aacacaataa gagcaataga
    15661 tgctttaggt gctataactg gtcaaatagg atttagtggt ggaggggtaa actatgctaa
    15721 taaagtttat ccgagtgtta taaattctga cccttacaat agccaatcat atggtgaaga
    15781 tagggagttt tatgtaagta atatcagtaa atttatagaa gaatctttaa aaaatacatc
    15841 aaataaagta aattatgcat cagatgaact tgatatgaca tcaaataaag taaattatgt
    15901 atcagatgaa cttgatataa catcaaataa aacagattat atatcaaatg aattgtataa
    15961 tttatctaat aagtcaatca aaggtaatat tcctataaag atggctgtaa taactaaaag
    16021 taatatgctg aatcaactgc cagatttagt agaattggag agggtgtttt caaaaattga
    16081 atttaaagta tgttttgata tgtttataac tgatacagct actttatgtg acatgtttat
    16141 accgtgtaca aatacactag agagtgaaga tataatttat agctcaatga ccaatccata
    16201 tataacctat aatgaaagag ctgtaaagcc agcacataaa ttgatggatg aatattactt
    16261 ctttatggag ctagctaaaa agatgggatt aaatgattat ccttttgtag aaaagagaac
    16321 ttatttagaa aaagtgattg aaccacttaa gcgttttgac aaaaatctgg atatagaaaa
    16381 acttaaaaac aattacttta ctatacataa ccctgttgct tgggaagata agaagtttga
    16441 gactccatct ggaaaatacg agctttattc agaaagtata aaaaacttgg gaataagtcc
    16501 aacacctgta tatataagca ataaatataa agaaatagaa gataaaaata tttcttttag
    16561 attattaact aatcatcatg cagatacttt atttagtcag cattttatgg acaaaaaatc
    16621 tatagctcaa gcatatataa atcggagaat ggctaaaaaa gtaggtattg aggacaaaga
    16681 tattgtgata ttgaggtcaa aaaaagcaaa aataaatgta caaataaata tagatgatgg
    16741 agtaggtaat tatatagtaa aaatgtatgt tggttggtgg aagaaacatg gaaatcctaa
    16801 tagtttaaca gacactggta tatcagattt tgggggtcag gtaacatata atgaaagtat
    16861 ggtagaaata atacgacaaa actagttaaa tttcattgaa ggaataaaaa tataattata
    16921 gaattgatta aaagttacta tttttacaaa actattgaag gagggatttt tgatggtaaa
    16981 ttattcctta gaacataaga atctatacat agaatttatg tgctcagaac tagaccacca
    17041 tgtagccaat gaaataaggg aagaaataga taacttatta agtgtaaatc aagtaaaaaa
    17101 tgttgtattt aattttgaaa atattaattt tatggattct tcaggtatag gcgtcatcat
    17161 aggtagatat aaaaagattt ctaatgaagg gggacgagtt tcagtaatta atataagttc
    17221 acgagttaag aagatttttg atttatctgg gttaaataaa attattggca tttatgatac
    17281 gtatgaagaa gctctaagtt ctttgtaggg aggagtatgt gatgaataat attatggaag
    17341 ttaaattttc ggcaatatct gaaaatgaaa gtttagcaag agttatagta gcatcctttg
    17401 cagctaagtt agacccaaca gtagatgagt tagtagatat aaaaactgct gtctctgagg
    17461 ctgttacgaa tgctataata catggatatg aagaagacag ttctaagttt gttttcttaa
    17521 gatgtgagat agaaggaaat actataaaga ttatagttga agatgaaggg tatggaatag
    17581 aaaatgtgga gaaggcaatg gaaccacttt atacatcaaa accagaactt gatagatcag
    17641 gaatgggatt cacagtaatg aaaagcttta tggacgatgt agaggtaagt tctgtaaaag
    17701 ataatgggac tagaattgaa atgactaaaa aaataaatgt gcctaaataa agtccaaaag
    17761 tgaggtttta ttatggaagt aactgttgcc agagaagaaa aaaaacctct tcttagtcat
    17821 gaagagactc tagaacttat tgaaaaagtt caaaatggag atgaagaggc aaaagaaata
    17881 ttaatatcaa gcaatttagg tctagttagg agcgttgtat ctagattttt aaatatagga
    17941 tatgatagag aagatttatt tcagttaggt tctataggct taataaaatc aatatataaa
    18001 tttgacccaa aatttaatgt aaaattctct acttatgcag taccaatgat tttaggtgaa
    18061 ataaagagat acttaagaga tgatggtatg ataaaagtat caaggtcgtt aaagcaaata
    18121 gctataaagg caaagatgga aagtgaagct ttaactaaaa aattaggtag agagccgagt
    18181 atagaagaat tagctaaagc aatagatgta gaaaaagaag atttagtaat ggctatggaa
    18241 gctaatttca atgttgagta tttgcaagga gttattcatg aagaggaagg ttctccaata
    18301 tgccttattg ataaaataag catgaaagga gaaagtgaag aagaaaaagt agtggataac
    18361 atattgttaa aagacatact tggaagactg gataagagag agcgtcaaat aatagttctc
    18421 agatactttg aagacatgac tcaaagtgaa ataggagaga tgctcaatat atcacaagtt
    18481 caagtatcta ggatagagaa gaaagtattg tctaagttaa aagaatatat atcgtgataa
    18541 tacttatgat atgtagaata ataaaatata ataaatatat tactaaaata aaatgtaata
    18601 actttttatg ttaagttttt aaattattaa aagatattaa aataataaaa agcattaaaa
    18661 aagtaaaaat ataaaaacaa ggtgtcttta tggaaaaata gattttttct ttattaggca
    18721 tcttttttat tttttgacca aactttagat agtcaaaata aataataggt ttattttttt
    18781 aaaattaaca aataatactt tagagaggtg tttagtatgg ataaaaatta taaaaaatat
    18841 gtagaccaaa taagcccaaa acctacatat ttaaaaaact atacacttgc atttattgta
    18901 ggtggaatta tttgtatgat agggcaagca ataaatgatt tgtatatgaa agttggaggt
    18961 cttgataaac taggtgctag ttctgcaaca tcaataacat taatatttat aggggcattt
    19021 ttaactggat taggagttta cgacctaata ggaaaaagag ctggagctgg ttctataatt
    19081 ccaataacag gttttgctaa ctcaatagta tctccagcaa tggagtataa aagagaaggc
    19141 tatgtattag gtgtaggtgc aaacttattt aaaattgcag gaccagtttt agtttatggg
    19201 ataggttctt ctattttatg tggaattatt tattatattt ttaagatgtt ttagatgatt
    19261 tttatttaat ttaaatttta ttaattatga agaaggtata aaatctgaaa atagctacta
    19321 aagtaactaa ttgaaaaata attctgcaga tttaattaat atttttaatt aatattaaaa
    19381 atttgaatag taaaatgcaa aattaaaaag gtgtgaaaat atgaaaaata aaagaattgg
    19441 aaaaagaaca gtcaagttag aaaataaacc aaccataata tcaactggaa ctatagtagg
    19501 tcctaaggaa ggtgaaggac cactaaagga ctattttgat atgattatga ctgacgattt
    19561 atatggtgaa aagacttggg aattagctga aagtaaaatg gtagagactg catctcaaca
    19621 agctatccaa aaggcaggta aaaaattgtc agatgtaaat tatatgctag gtggcgattt
    19681 aataaatcaa atagttccag catcctttgc agcaagagaa ctggctattc catttttagg
    19741 tatatatgga gcttgttcta caatggcaga aggtctttgt ataggttcta tgcttataga
    19801 tggtggtttt gctgatttag tactgtcagg tacatcaagt cactattgta cagcagaaag
    19861 acaatttaga ttcccacttg aacttggaaa tcaaaaacca atgacagccc agtggacagt
    19921 tacaggagca ggaagtgtat tattagctcc taatggagac ggtccaaaag taaagtatgt
    19981 tactgttggt aaagtaatag atgagggtat tgatgatggt aataatatgg gaccagctat
    20041 ggctccagca gcaatagata ctatctactc ttactttgaa gatactaatg atgacccaaa
    20101 tagctttgat ataattgcta ctggagattt aggtactctt ggaaaacaga tagcagaaga
    20161 ctttctaaaa gaaaaaggtg tagatatatc aaaagtttat actgactgtg gtatagagat
    20221 gtttaacctt aaagaacaag atgtccactg tggtggaagt ggatgtgggt gttcagctac
    20281 agtgtttgca ggatatatat atgataagtt gagaaaaaaa gaatttaaca aagtaatgct
    20341 tgtatcaaca ggagcactac tttcaccgac aagtacacta caaaagcaga caataccatg
    20401 tgttgcacat gcagtagtaa tagtaaatga gtaggaagtg atataatatg ttaatggact
    20461 atgtaagagt atttattgta ggtggaatta tatgtttaat agcccaagta atgatggatt
    20521 atttcaaaat gcaaactcca tatgttatgg taacgtatgt aacaactggt gtagttttaa
    20581 cttttctagg actttatgag ccattagtta agtttgcagc agcaggtgca acagttccta
    20641 taataggatt tggatattca cttgctacag gagtaataaa atcaattcaa agtgatggtt
    20701 ttttaggtat ttttttagga ggtacaactg ctacagctgg tggaatagca gcagctatat
    20761 tctttggata tattatgtca gtagtattta ctccaaaagc aaaaccataa atctttatta
    20821 aaagaataat aagtttttga aataataact ttttataaag caggtggata tcttttagat
    20881 tccacctgtt ttttatataa agaagcgttt acataattga agttgacaat tatttacaac
    20941 atagtgtagt attatattat caaaataaaa tgaagtgaag taggggataa agatggtaac
    21001 tattaaagat attgcaagga atctaggtat cagttattca actgtttcta ggtgcttaaa
    21061 caataatcct aatgtatctg aaaaaacaaa aaagaaagtg gtagaagagg caaatagact
    21121 agggttccat tttaatgtaa atgcaagaaa tttagcaaaa aaagaaacta acaggatagg
    21181 ggtaatcttt tcaaataatt ttaatcatca agatactaga aagtttttta gtgatataat
    21241 ggatagttct ataaattcta ttgagactaa taaatacgat tttataatac agcctaataa
    21301 taacattagt ggtgatagta atgtatataa aatggtaaat ggacaaatgg ttgacggatt
    21361 agtaattgtg tctcaatcaa taaaaaaaga agagtatgat tttttaaaag acaataattt
    21421 tccacatgta tttatatatt ttaaaccatc ttttatagga gaagtagata atttcttttg
    21481 ggacgataac gtgtatggag gttatatagc taccaagcat ttaattgacc atggtcataa
    21541 agatataata acagtaactt cagatgataa gagttcaaaa atgcatgaag atagaaccaa
    21601 gggatatttg aatgctatgg aagaagcaaa tttaaagaca gaagtcatta aatctaaaat
    21661 ggattttgaa tctcaagttg aatttttaga aaaatacatt gataaaataa aaaaagcttc
    21721 agctatattt gtacaacaag atgtaccagc gatttctata atacaagagt taaaaactac
    21781 ttatggtata aatgtaccag aagatatttc cataataggg tataataata ttgaattaat
    21841 atcatatttt aggtcgcatt tatcaacaat agatgaccca agagagcaag ttataaaaaa
    21901 tggagtagat agtttagtaa atataataaa taaaaagagt acagaacaca ttccaagaaa
    21961 attatatcca aggttaataa tacgaaatag cgttaaaaga ataaagtagt aagcttttaa
    22021 gtatcaaacg tttattaaaa atattataga ttatgtgaag ttttttaaca aataatataa
    22081 ataaaaatta aaaggagtag gtgtatataa gtacattgta ctcctttatt tattgagata
    22141 gtttttataa tatgaatgaa taattttatt caattatttg aaaatccgtt attttaacaa
    22201 tataaattaa taaattattt gtattttata tcatgaatct ataatatttt tttgattcat
    22261 aactaaaagt atataaaaat gatgaataaa tatatattat acattatcaa tgataggtta
    22321 tataataaaa tcactaacaa ttatattata agaaaattat aaaaattaca aggaggtata
    22381 gaaatgaaag aaaaagcagg gattagaaat gccataatag cagcctttat aatcacaata
    22441 ttggcagaat taattggacc tatttcattt aaagttatgg gaattaatgt aactttactt
    22501 agtatactat gggctatatt cataggaatg gctgtttctc ctcatttatt agggagagtt
    22561 ataccagcct taaaaaagtt tataggggat aatgaaataa atgtatctcc atatttatta
    22621 tctttgacac tatatccatt agggataatg tttggaatta atgcagggcc taaagttggt
    22681 atattacttc aagcaggacc agcactttta ttacaagaat ttggtcatat gggtacaatg
    22741 ttgatagcat tacctgttgg agtagcatta ggtcttggaa gaagtgcatt aggtggtact
    22801 ttttcattat gtcgtgacac agcactagga attattggag ataagtatgg attaaactca
    22861 ccagaaggta taggaacact aggaacttac ataagtggta gtatatttgg aacattatta
    22921 ttttcatttt tagcaccagt gggtgtactt ttaggatttc atccatatgc tttagcaatg
    22981 gcatcaggaa tgggtagtgg ttcaatgatg ggagctgcaa cagcatcatt aatgaataca
    23041 gttccacaaa tgtcagagca gattttagca tattcagcta ctagtggatt gattacagct
    23101 gttactggaa tatatataga attattctta tctttaccag tagccaacta ctattatagc
    23161 aaagtagctc cagttattga aagatttaga ggaagaaaaa caagaagaac aagtaataaa
    23221 ttgtaaagga ggatatatat atggaaaaaa cttttaagac tattatggag cagataaaaa
    23281 tagtattgtt tatatatatt attatttgct ttggacaaat gatagcactt aagactcagg
    23341 ctcaagattt gttgattgga agtgtatctg gatttttgtt ggtgataatt gctattataa
    23401 taaagaattt tgttaaaaaa cctaatttac caggttttgc atgggctaca ttagttgcat
    23461 ttgttttatc gcttccaata tctccagtgg gggatatcat agcaacaaat gtagggaaaa
    23521 tcaattttat gtctacagta acaccattat tagcttttgc aggaatctct gtaggtaatc
    23581 aattagatat attaaagact ttgagttgga agttagtagt tatatcattt atagttatga
    23641 catctactta ctttggttct gcttttatat ctcaaacagt tttaagatta aatggaatgc
    23701 tttaaggagg tagtaaatat gaatgaatta aaagcaaaaa ttaaagagga aataaagtta
    23761 ttatctgaag agaaaaagac atcatttgaa aaagtttctg actatttatt ttcaaatcca
    23821 gaattagcat ttgaggagta taagtcacaa aaagcattat gtgatttgtt agaagaaaat
    23881 gaatttaatg taactaaagg tgtaggagga ttagaaacat cttttgaagc tgtgtattca
    23941 aatggaacta atgggaaaac agttgctttt ttagctgaat atgatgcact tccaggaatg
    24001 ggacatgcat gtgggcacaa tatcataggg acaagttctg ttggtgcagg tatcatatta
    24061 aaagaaatca tgaaaaaaca taatatagaa gggactgtaa aggtttttgg aacacctgca
    24121 gaagaaagag taggtggaaa aataactatg attaaagaag gtgtatttaa taatgtagat
    24181 gctgcattaa ttttacatcc aagtgatgct tcaatgcctg atgatatatc atttgctcaa
    24241 gttaatttaa aatttgattt tactggtaag gcatcacatg ctgctgcttt tccttgggaa
    24301 gggaaaagtg ccttaagtgg agtaatagca ctatttaaca gtgtaaatag catgagatta
    24361 catcttaaag attatgcaag agtacatgga attataactg atggaggtag catacataat
    24421 ataattcctg aaaaatctac agctatattt aatgtaagag ccttaagtat agaatattta
    24481 aatgagattt gtgaaatgct taaaaattgt gctaaaggag ctgcaatttc tactggaact
    24541 aatgtagaaa tagttcaatt agatgaaata tacaaagaga ttaagaatga ttctgaatta
    24601 gtgaatatag ttagacaaaa ttttgaagtt ttgggtgaag actatgttga aagagattta
    24661 agtcaaggta taggttcaac tgatacagga aatttaactc atgagatacc tgcaatacaa
    24721 gcatatataa aattaaaaga aaatactgca actcatacag atgaatttgc tgttgcagct
    24781 ggtggtgaag aaggcagagt agcacttata aaagcaatta aggtgttagc aatgtgtgga
    24841 gtagatattt tgtatagtaa ataatatata attttaaaat atattaagaa aggaaatcgg
    24901 attagacatg atattaaaac aagtaataga ggtttatgac attttagata aagcaaatgc
    24961 taatggggaa gaagtaaaaa cttacttaca aggttatggt gaagtagatg taactgtaaa
    25021 agaattgtct tcaagtaaag gttcaacaga tttagtaaag cttacaattc ctggaaaaaa
    25081 tggaaaatta aaaggtggag atgctcctac tcttggtatt ttaggtagac taggagggat
    25141 tggagcaaga ccagaagtta taggttttgt ctcagatggt gatggagcat tggtagcaat
    25201 tgcagtggct gctaaacttt tggatatgca acgaaaaggg gatatattag atggtgatgt
    25261 tgtaatttca actcatattt gcccagatgc tccaactaaa gaacactatc ctactccttt
    25321 tatggattct ccaattgata tgatgactat gaatgaaaat gaagttgata gtagttgtga
    25381 tgcaatactt tctatagata ctactaaagg aaatagagta atcaatacta gaggatttgc
    25441 aatatcgcca actgttaaag aaggctacat attaaaaact agtgataacc ttttagatat
    25501 tatgcagact gtaacaggga aatctccatt tgtattccct ttgtctattc aagatataac
    25561 accatatgga aataatctgt atcacttaaa tagtatttta caaccagcag tagctacaga
    25621 tgcaccaata gtaggagtag caattacaac agagatgcct gttgctggat gtgctacagg
    25681 tgcaactaat tttagtgatt tagattcagc tgggagattt gcaattgaag ttgccaagct
    25741 atttggtaga aataagtgtg atttctatga taaagaagag tgggaaatgc ttattaagag
    25801 atatggcaaa ttaaatagat ttcaaacatt tggtatacag taattagaaa aatattaagt
    25861 ttatataatt tgtgatataa ctatttaaga atatataaac aattaggaat aaaagtaaga
    25921 ataaagcact tagttaaaaa ctttaaaaat aaaatattta tttgtttata taaaaaggag
    25981 gattctggtt attgggattc tcttttttat gaatacaaat aatatattgt aattataaaa
    26041 aaaggagcgt ttcaaagtga tttttttaat caccttgaga caactcctta tatttttatt
    26101 taaattctat tttttattaa gttgctggag ttccaccacc atcaggagga gtagtagagc
    26161 caccaccatt atttccacca ccatcaggag gagtagtaga gccaccacca ttgtttccgc
    26221 cattatcagg aggtgtagaa ccgccaccat tatcacctgt gttattattg ttattattat
    26281 tttcttctgg ttggttattg tctggttttt ttgtatcttc agatgatggt tgagaactta
    26341 ctttagtagg agcagtacct tcaataaatg ctgctttaga gccagaagat tgctttcctc
    26401 catcctctaa attaattttt gtaaaatata catttttagg aacttcaaat tcagtaaccg
    26461 ttaaattagc atgtattttt tccataattt tttcccatag ttttgcagta ctagtacttc
    26521 catgtggtac ttctctacgt tttatagtgt taccagaatc atcttttcta ccagcatcat
    26581 ctccaatata agttgcacca acataataag gagtgtaacc gacaaaccaa gcatcatatg
    26641 cactatttgt agtacctgtt ttacctgcta caggcattcc ctttggaagt gtagcaacac
    26701 caccagtacc ttcagtaatt acgctttcta acatatctgt aacaacataa gctacttcag
    26761 ggtctacaac tttatgttct tcaggagcat ttttaactaa taattgacca tcaaaggtag
    26821 ttatagtagt aaaaatagaa ggttctacat aaataccgtg atttgccaat gtaccataag
    26881 cagcagccat ttgaagtgga gtaataccat tcgccatacc acctagtgtc aatgatggga
    26941 attttctatc acttgcttca gatgaacctg agtgattatt ctttaaagtt gttataccaa
    27001 agttctttaa gtaatccatc attatatcta cacattcatc ataagagtct ccaagcattt
    27061 cagcagtttt gacagctatc gtgtttgaag atttagcaag tgctttacgt aaactcatag
    27121 aaccatgacc agcagtagta gttcttggat tccatttatt attttcttca aacttatatc
    27181 ctcctcgaga atcacttaga gcagttgctt gtgttatttt tagagtatca atagctggcg
    27241 tatatattgc taaaggtttt atagaagaac ctggctgatg agggttagta gcacgattta
    27301 aagtttttct accacttata tttcttcctc cagctagtgc tcttatttca ccatttttat
    27361 aatctaagat taccattgca gcttgcggtt ggctaactcc atcaggaccg accatatgtc
    27421 ctgggaaatt gctatttcta tcaaattctt cttctaaagc atcttgcatt ttagggtcta
    27481 tagttgtgtt tacaattaga ccaccattgt agaataagtt ttgagcttca tcatttgtat
    27541 gtccttgttc cattaaagca tttataactt cactttcgat aaggtcttct acagatgaag
    27601 aaactttatc tgatgattga ggcaatttta tttctatttt agctttgatt gcttcatcat
    27661 attcactttg ttttatatat ttcaattcta acattttctt taaaacaatt tcttgtcttt
    27721 ttttagcttc aggattactt acagcctttc taacaactaa tgtacctgct tttaattgtc
    27781 tataagtatc attagatata agctcccaag attttatttt ttcaatcata tcaaagtcca
    27841 cttgagtagg gtcatctaaa tcatcatcag ttgtgttaac aaaaaataat aatttattct
    27901 ctaaatcctc cttagtttca tttccctcta acttagaagt tttataggct gaaaatcttg
    27961 atggattttt agttgaacca gcaagaagtg ctgactctgc tagagtaagg tcagctgcag
    28021 acttatcaaa ataaccacgt gcaccagcct cagcacctgc tagacctcta ccaacgaaga
    28081 agttatttaa ataagtttct agtattttat ctttacttac tgttttactc atctcatgtg
    28141 catagtaaat atcctttatt ttacgaggta tagtttgctg ttcagttgtt aacagcattt
    28201 tggatacctg cataggaata gtactaccac cttgcttagt acctgtaagg gtctttaata
    28261 ctgacctaaa taaaccttta atatcgacac ccttatgctc ataaaaacgc tcatcttcta
    28321 ttgcaattac agcatgttga agataagggg aaatcttatc taatggaacg ggtgtctttt
    28381 tatttacact tggagctgta gataatgttt ttccattagc gtatttaata gtagttgttt
    28441 ggttcgtctg tttatctaaa acagcttctg taactggagt tacatctctt agtgacgcaa
    28501 atactagacc agcagtacct gctgtaccaa caactagtaa aactaaaaat actatcccaa
    28561 atactcttag ttttttaaat ttatcgcttt ttttagattt tttcttgttt tttgttgaac
    28621 gagtcttgtt agctgaagtt ctattaattg gtttatttgt attaccagat gagctaactt
    28681 tttttctcct gattttattt ccatttttat cattatcgtt attcataaga ttttaacctc
    28741 cttacttaat gcaatattat aacacaaata ggagtagttt tgtaattaat agaaaaaatg
    28801 aatttgctat aatattctgt acattgtata tgtataaacg taatatatta
//
LOCUS       DAYAPA010000043        28026 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.043, whole genome shotgun sequence.
ACCESSION   DAYAPA010000043 DAYAPA010000000
VERSION     DAYAPA010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 28026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28026
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.043"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            27..2261
                     /locus_tag="ACGI59_001723"
     CDS             27..2261
                     /locus_tag="ACGI59_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFG9739509.1"
                     /translation="MQNHNYIKMLNKKVEVLSKELDSRNFKLKTLNEIDFFSKIPNRE
                     FMYQHIDDCISKNIHIGIMLLNIDNFKLFNDIYGYLVGENLLITLTQNLLHFIPESKG
                     IVAYLKGEGFLIVLPNVSNKELNNMGATIIESARELKIRLPNEKEIYSNVTFSIGSTI
                     WNGEPNMTTLMLFNQVYSALKKAKSEGKNRYILFDFKDNSFYNKLENDKEFLSEISHV
                     LQTKQIEVFYQPLYNIKNNTIVGCEALARWRHPIKGLLTPDKFIPLFEQFGLITELDL
                     HLFEENCKNIRRWIDNNDVFVPICCNFSRLHFFNPNFPSMLKGITEKYSVSTSNFSIE
                     ITENILIENTNTIIQQLAELRSYGFSVSMDDFGSGYSSFGMLQNLPFDIIKIDRIFLS
                     HNLNDFKNTTILYSIINIARALGMLTVCEGVETEQHVEFIKSINCDIAQGYYYAKPME
                     CKEFEKLLSKENNSEKMFIKYSDIGKSLIEKIFDGFFIMQDMKKLRANVTEDIQWFDF
                     YNKTEITSFPKVIEHFEKHIKGKKFSILYKSITPHNTESDSLLISGEAVLSDEASEHQ
                     KYSNFYFSANCIISENKLLLAKLHLDLIKTAGYINEFESYSIPKMTNTHLDESNILSQ
                     FYSMLPVGIIRYDLFGDMIITYMNEEMFDIIGYTKEQFFGEMGGNLRAIVHPDDLDFV
                     YKKSLDMIDTNKIEPFLYRFIKRDGQIVTVMYTQCNLSAIDGRSIAQGMYVDVNKIKN
                     LTKI"
     gene            2292..3137
                     /gene="dccA"
                     /locus_tag="ACGI59_001724"
     CDS             2292..3137
                     /gene="dccA"
                     /locus_tag="ACGI59_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP diguanylate cyclase DccA"
                     /protein_id="HFG9739510.1"
                     /translation="MFKEIFLRTFPGFLIIRDSNYRIIFINDNLKNLIKSYMQDNPLG
                     MTNIEIAKKLPDNIAKFFTDSHNIQLDWEKNYPYDKISNWILEFKKDTTSYWNVLEYK
                     VDVDEKTYIITMANDITKLYEENKRNLHYSITDPLTGAYNRKYLNDRFDIFIGDYIVL
                     IDLDNFKMINDYEGHNVGDKILCDFVSLLNKELINSTSIIRLGGDEFIVIFSSDVDKN
                     YVYSQLEALRENFLKVFSKYKYLSFSYGVDTVKRNLKLTIVELDKKMYKNKEKNKKKF
                     DKNDY"
     gene            3377..5110
                     /locus_tag="ACGI59_001725"
     CDS             3377..5110
                     /locus_tag="ACGI59_001725"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFG9739511.1"
                     /translation="MSILLKKAPKLAKHIITSFYINRDINEVLKYLCENVTWIGPGEQ
                     EFLTSFNEIKNYFYAGQDEIPSCDINNDIFETVSEDENRCMVLGRYTVRTKENAQMIL
                     EVNQRCTFEIIEDRGKLLIKHMHISNPYGEMQLDEYFPTKIGTQSYDYLQRLLKEKTE
                     VIEMITNNINGGLKGSNDDSTYSFFYVNEGLPKILGYTYDEFMEMSGGSAVGAVYPPD
                     LPKALEDCERCFAKGPTYSSEYRIRKKDGTLMWVLDSGMKSLNSDGIVKINSIITDIT
                     QLKNIESELKLERERYRIALQNITDIMFEYDIENDTLIKYQRVEIDKKIELENFETKN
                     YSKLLESGKIIHLDDIGKLLEVLHGNLRETIEIRQVNSLTKNEWRWIRVQCSVIYDSD
                     HNPIKTIGVLKDITEDKSKLESLINQAQRDPLTQLYNQRVSQNLIEEYLCSSDSKNND
                     ALLVIDIDDFKTVNDTFGHLEGNEVLVAVSKILLHNTHDKDIVARIGGDEFTIFIKSL
                     TKDLIIKITNDILNDASKIKVKDNHKITLSIGIAFTDDSTKLYKDLFSKADKALYLSK
                     ADGKNCYSTYE"
     repeat_region   5442..5922
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=5508..5530
                     /rpt_unit_seq="ataccacttagttaatataaaac"
     gene            complement(6452..6556)
                     /locus_tag="ACGI59_001726"
     CDS             complement(6452..6556)
                     /locus_tag="ACGI59_001726"
                     /inference="COORDINATES: protein motif:HMM:NF024729.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFG9739512.1"
                     /translation="MDDYIYYYNNFRYQNRLNSMSPFEFRKYLSTERA"
     gene            7181..7426
                     /locus_tag="ACGI59_001727"
     CDS             7181..7426
                     /locus_tag="ACGI59_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9739513.1"
                     /translation="MNQKKIKISIKMVIIGIFSVIIAFVLSRLFVEYIDELLRSNWQY
                     QLFESESSYIIFANCIMFSNILLEIFLIYICRKFRKI"
     gene            complement(8179..8325)
                     /locus_tag="ACGI59_001728"
     CDS             complement(8179..8325)
                     /locus_tag="ACGI59_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9739514.1"
                     /translation="MVFETFDKTIELYPNAKPIFHSDIGFRLDDESFEEYLGRVFRKR
                     CKGI"
     gene            8421..8744
                     /locus_tag="ACGI59_001729"
     CDS             8421..8744
                     /locus_tag="ACGI59_001729"
                     /inference="COORDINATES: protein motif:HMM:NF024502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFG9739515.1"
                     /translation="MKDRECSFSPNTIRNRKNMIKNQIEVFFKDMKLIDITPSILQSY
                     INNIYNEHMLNSIKNQVDFIKSVLKESYRLKEISENICDFVTTPIKKVVQLLSFIQNK
                     KLNYY"
     gene            9611..10966
                     /locus_tag="ACGI59_001730"
     CDS             9611..10966
                     /locus_tag="ACGI59_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFG9739516.1"
                     /translation="MDTKISINENMSLGKRFFKYLAPSVVAMWVFSLYTMVDGIFVSK
                     GVGELALAAVNISMPFINFIFAVSLLFSTGASTIIAIYLGKKDIKSANEVFSFNLVSI
                     IILSIIILAITFFNLDRLALFLGATESTIGMVKDYLGIIIFFNGFFIVSYSLEVIIKT
                     DGFPILATVGVIISALTNIILDYLFVIEFGWGVKGAGIATGLSQVFSTIFFLIHFLRK
                     NSTLNFSKFRIDFKTLRKIVFIGFPDSTTELSCGIVVLLFNLSLTKYIGENALIYYSV
                     INYINTLVLMTMMGITQGMQPLTSFYYGAGNIDNVKKLLKMGIKATIIASVAVFAICM
                     AFSGPIVSLFIHPEETMLFNEGVRVFKIFSISFLLVGINVIISGFFVSVEKPSISTVI
                     SLGRGLVIVVLSLISMILIFGGQGIWMTTIVSEFICLILSLVFLKKNFSTLDSNLNKV
                     A"
     gene            complement(11084..11743)
                     /locus_tag="ACGI59_001731"
     CDS             complement(11084..11743)
                     /locus_tag="ACGI59_001731"
                     /EC_number="6.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphthine--ammonia ligase"
                     /protein_id="HFG9739517.1"
                     /translation="MLKLADEKFVMSFSGGKDSILALNRMLKKGYKPVALLTTISEEH
                     GKSWTHNLEYNMLKQVSSNIGLPLLVAECGVEGYEESFERALIKAKNMGATICAYGDI
                     DIESHRKWDTDRCEAVGMKVDLPLWQESREELVYEFIDSGFCSVVTKVNLKHLGEEFL
                     GKKLTRELVEKIKNAGADPCGEHGEYHTFVVDGPIFKKPVEYEVKGTLIKDGYGYLEI
                     K"
     gene            complement(11819..12247)
                     /locus_tag="ACGI59_001732"
     CDS             complement(11819..12247)
                     /locus_tag="ACGI59_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFG9739518.1"
                     /translation="MTKLWAIIFALLAGGSTALEAFINGELGKSTTALVATFISLTVG
                     AIFFLASIIVTGDLKCLVSINGFNYKLLLGGVFGGLIIYFTVKAIPNLGVSNTLILTL
                     VSQVLVGFFIDSVILGQETMHLYKYIGVILLILGTFFIVN"
     gene            12399..12947
                     /locus_tag="ACGI59_001733"
     CDS             12399..12947
                     /locus_tag="ACGI59_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HFG9739519.1"
                     /translation="MYTNFNIDFNNFNKKENAIKFMLMGVLLIILGLLCLTFKTLGIK
                     LISWTFGIALLFFAYLNLKNINELKRYATKEEIKPSINIQWVLIIACILLFVFPQKIQ
                     SIFSLFLGFYLIFNQLVALVNSKNNPYSKFTTWNIVKILFGICLILSPLFLSRFIVSI
                     MSFFIILFGLVLFFSGNTARKY"
     gene            13066..14001
                     /locus_tag="ACGI59_001734"
     CDS             13066..14001
                     /locus_tag="ACGI59_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893027.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFG9739520.1"
                     /translation="MDKFKYAFDNKRYHTWNYYLRNTFGEKVFKVSINAGFTCPNIDG
                     SLGYGGCTYCSKEGSGDFAGNPKDNLISQFYNIKEMMLKKWPHAKYIGYFQAYTNTYA
                     PLEVLKEKYETILELEDVIGLSISTRPDCLPDDVVEYLSELNKRTNLWVELGLQTIHD
                     STSKIINRGHDYKTFVEGVEKLKSKNIKVVVHIINGLPGEDYNMMMETAKAVGKMGVD
                     GIKIHLLHVIKDTPMEKMLQNGMLTLMEQDEYINLVCDQLEILPETMIIHRLTGDGKR
                     DELVGPIWSLKKWEILNKIDDTLKARNSYQGCKFV"
     gene            complement(14272..15216)
                     /locus_tag="ACGI59_001735"
     CDS             complement(14272..15216)
                     /locus_tag="ACGI59_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFG9739521.1"
                     /translation="MGEKSKGYVFIAIAGLLWATLGLFGKFLMGNGLTSEQVAFTRLF
                     FGFIVLGVYSSIRTPQILKINKKGIIYSVIIGIICQAMFNLCYFKAIDIAGVSIAAVL
                     LYTSPLFLAIFSKLCYKENITRSKLFSLILCFIGAIMAVTGGRLDFQGLNAFGLLLGV
                     LSAIAYALMPTISKNALKEFSSSTILVYSFLFGAIFMIPSSRPWEILNYAKDLDVLSC
                     MLMLGIVPAALAYIFYAAGISKGVELSVAGVVASVELVGSVIIGCTILGESFSLGKLF
                     GVMLMLISAVVALNLSYDEIRIFYKSNKLKQIEKTESI"
     gene            15534..16964
                     /locus_tag="ACGI59_001736"
     CDS             15534..16964
                     /locus_tag="ACGI59_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFG9739522.1"
                     /translation="MNLDLEFYQKILEASHDEICVSDDKGIIIYCNKAFEENYGLKKE
                     DILGKNVSFLEDSGYSTKSPIPIVLKTKTKFSLEQDTQTGKKLIITATPIFDENGHLE
                     FTVENCRDITELNNIKNKLEDTKKQVKKYKFEVETLYRTALRIEDTVIMDGIVMRPII
                     NTVNHVSKTDVSVLLLGESGTGKSSLARYIHHNSNRSNGPFITINCATISPQLLESEL
                     FGYTSGAFTGASTKGKVGLVELANGGTLFLDEIGDIPQNLQAKFLQLIQDRTFTPVGS
                     LKNKKVDIRIISATNVDLVSKVKEKKFREDLYYRLNVIEIKLPPLRERRDNLVEIIKY
                     YFNRYSSDFNLNKTISKEAMDAIANYRFPGNIRELQNIIQKILLTCTDNHITIDDLPN
                     ILTKNIHITNNGNKTHISQINKVIISDSKSTNYKNKNFDTLIKEYEKNIILDAYEKFG
                     SSYKVAKHLEISQSKANRLIRKYTNT"
     gene            17164..17748
                     /locus_tag="ACGI59_001737"
     CDS             17164..17748
                     /locus_tag="ACGI59_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HFG9739523.1"
                     /translation="MLMNTHFIMAKSILDNIDENKTFFISEKNFIYGNIKPDAFSKYK
                     LKKHYLDESFNMIVSKINYLCSLNIDSLSKYFSVSRLSQELGVICHFLCDFFCVAHSE
                     RWEFKHSLNKHVTYERELGAFAKDVDLSKIKCGTINSSFEKFFTKLYSDYKKKTDYMN
                     DLKFSAYTCNSVINYILDSILSNTIKSYHIANCG"
     gene            complement(17852..18631)
                     /locus_tag="ACGI59_001738"
     CDS             complement(17852..18631)
                     /locus_tag="ACGI59_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFG9739524.1"
                     /translation="MIKHIFCDLDGTLYENGTITKEDIVAIEEIEKKGVQFNVATGRI
                     FKQAHNIIKDSLDMNGYYVCENGSFIYDKDYNVIFKRTIDDNLVKKVIDRFESSDAQL
                     YFKYKGDVIVGSDTTAFRHYSSDFIVDPDFEKRSSFDNLIGNIGVCSENLEELSRIEL
                     YLKSEFSEVLEIYFSGTYTLNIVPKSVSKRGSIEHVIKTLNVSPDEVATIGDSPNDIC
                     MLEGFKYSFAMSKAREDVKQSANYVVDSVKSAIDVIMEINS"
     gene            complement(18689..19945)
                     /locus_tag="ACGI59_001739"
     CDS             complement(18689..19945)
                     /locus_tag="ACGI59_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HFG9739525.1"
                     /translation="MKAEIISVGTEILLGDIVNTNSQFLAKELASLGIEVYHQSTVGD
                     NKQRLLECFDESLKRSDFVITTGGLGPTGDDMTKETAAEYFGQKLELHKPSLEVLESF
                     FVKTGKKMAENNMKQVYFPKDAIVLKNNNGTAPGAILKKDGKFIIVLPGPPREMKAMF
                     NESVKPYLQQFTNEMLVSKTLRLYGIGESNLELEILDIINEQTNPTVALYAKELEVTI
                     RITAKAENEREAFKLIKPVEEKIKSRVGKYVYTEGDISISEGETALEDAVSKLLVEKN
                     LTIAVAESCTGGLVSSSLINYPGISSVFLEGCVTYSNDSKMKRLGVKRETLEEFGAVS
                     EQTAIEMAEGIAKGLKANIGISTTGVAGPGGGTKEKPVGLVYTAIYINGKTIVKKNIF
                     NGDRRKIRLRATRDLLNELRIQLEKL"
     gene            20203..21102
                     /locus_tag="ACGI59_001740"
     CDS             20203..21102
                     /locus_tag="ACGI59_001740"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFG9739526.1"
                     /translation="MKIAVIMGGISSEREVSLNSGKEIYNNLDKNKYEVVKVIIDDKK
                     DIFTKIPEDIDFAILALHGKFGEDGCIQSILETMDIPYSGCGPLCSGMCMDKNITKKM
                     LRDSNLPTAPWVLVKSVDEIDYDEIDNIGYPVFIKPNSGGSSVATFFIHSKDEVEEAV
                     RKGLEVDEFVMIEKYIPGGEYTSFILNGEVFPTISIKSDSGFFDYEAKYSVEKGAKEE
                     VVYLDEELQKRVNEISETCWKIFNCKAYVRVDMIISEGIPYVLELNTLPGMTQTSLIP
                     RSAAARGIKYSELLDKLIEYSLN"
     gene            21126..22574
                     /gene="ddlR"
                     /locus_tag="ACGI59_001741"
     CDS             21126..22574
                     /gene="ddlR"
                     /locus_tag="ACGI59_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator DdlR"
                     /protein_id="HFG9739527.1"
                     /translation="MIFSNLKLNDNEPIYIQLKNYISDMISKGLIPDNSKLPSTRELS
                     QLLQVSRNSVVLTYEELKSEGLIYSISGKGTFVKSKNKSSNTTWSLNWDCLENTYSKK
                     ANELDIIKSEIPWSSDLISFKSISPDGDLFDMEELKKSFLNRISLEGHKLLNYGYAQG
                     YKPLIDYLLEYMTNKGADISNKDILITNGFTEGFDIIMSSFTQEKDYIICENPTHNTA
                     IKIMKSHNLNILGVDINEDGLDFNMLEDNLVKYKNKIKFAYITPSYHNPTGIVMTPEN
                     RYKFYNLMKKYNIAIIEDGFNEELLYNSSHIFPICSLDNMNNGVVYIGSFSKILFPGM
                     RIGWIFADKKVINKLESVKRCRNIHVSFLDQGILFDYLSNGGFEKYIKKIRKFYGDKF
                     NFAYKCIKKYIKTEYILGDGGLHIFIKLKNIDTRVLLEKCYEKNVIFMPGDLFFTDNS
                     GFDTLRLGFSRLSFEDIEIGIKIIGETINEMF"
     gene            22657..23439
                     /locus_tag="ACGI59_001742"
     CDS             22657..23439
                     /locus_tag="ACGI59_001742"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HFG9739528.1"
                     /translation="MSKKSKRRKINKLKLTIVITVLFLALLSIYEILFSNPNKKGNIK
                     SQETMQNKNNTTDDSKNNQSSTKKQEDNNIKPTFSYSSIPDNIKNKMIGKSMPTDEPI
                     SFDSLSYLKLTYYGFDEKTHQGEMIVNSELAPEVVDIFKELYEKKYPIEKIKLIDDYD
                     AVDEKSMSDNNTSSFCYRTIAGTNVVSNHGKGRAIDINPLQNPQVSGNDVTPKVSTVY
                     ADRSSTKFGMIKKGDDCYNAFVSRGWSWGGYWKNPDYQHFEK"
     gene            23465..25384
                     /locus_tag="ACGI59_001743"
     CDS             23465..25384
                     /locus_tag="ACGI59_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFG9739529.1"
                     /translation="MNLMTLENISKSYSEKKLLENISLGINEGEKIGFIGVNGTGKST
                     LLKIIAGVEESETGNITKTNGIRIEYLPQNPTYNENSTVLEQVFNGTSEELKLLGEYQ
                     EILEKLNSNFTEELNKKLLSVHEKIDALNLWDLESEAKSVLTKLGINDFNQKIAELSG
                     GQRKRVSLASALITPCEFLILDEPTNHLDNDTIDWLEEYLNSRKGSLLMITHDRYFLD
                     RVTNRIIELDKGRLFSYDGNYSIFLEKKMERLSLEASIEDKRQNLIRTELAWVRRGAQ
                     ARSTKQKARLQRFDELINRDSFKPDENLDISVASSRLGNKIIEIHNISKSFEENTVID
                     NLEYTLTRTDRIGIIGKNGMGKSTLINILNGEIEPDSGYISIGETVKIGCFSQDDSHM
                     NIDMRAIDYVKEASDYIETSDGTRITASQMCERFLFNGTMQYTLIGKLSGGERRRLHL
                     LKILMTAPNVLLLDEPTNDLDIETLKILEEYLDEFKGVVVTVSHDRYFLDRICNKIFA
                     YEGNGKIHIYTGNYSDYLIYREIQGIEFEESKRESVKKDDSTTPKKEKPKNDKKLKFS
                     YNEQREFENIDSDIEKLESKIATLDESTAKFSTDFTKLQEILDEKSKLEKELEYKYER
                     WEYLNNLADEIANNK"
     gene            complement(25626..25973)
                     /locus_tag="ACGI59_001744"
     CDS             complement(25626..25973)
                     /locus_tag="ACGI59_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HFG9739530.1"
                     /translation="MSKSNDVLDIVFKITDGLEYEVAENRVVTILEKQDHKIQKFFRK
                     LKARIPKYKKIELDEYSSFVFLQIDGQRTVREIGKNLEAKYGEESHPLYERLLVFLNH
                     IEVNCHYIERLDI"
     gene            complement(25960..27888)
                     /locus_tag="ACGI59_001745"
     CDS             complement(25960..27888)
                     /locus_tag="ACGI59_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OPT family oligopeptide transporter"
                     /protein_id="HFG9739531.1"
                     /translation="MNKKLPKGAYGEVSGKDYVPYITDKSRTGGNVAVLIIGIILAAI
                     FAASTTYSGMKAGLTVAAGIPGAIIGSAFVGAFARSKGILGKNLIQGMSSGGESVASG
                     FIFVLPAVILIGSQITFLEGLAVGVGGVLFGIGIAAIVHNYLIVEEHGKLMYPESMAI
                     SETLVASEAGGDSIKYMGIGFVISGFITVLTGSFLNVANNVMSLVGSKFYKWKFDIEV
                     NPLLLGIGFIVGLEVSLTMFAGSILSNFGIAPLIGYFTDMAKDGAMVWNNPAMPLNQM
                     DVGAISSSYVKYIGAGMMLCGGIIGAIKLIPTIIASIKETLKAKSSAGEGEEGSSIQM
                     ILLLGGVVIGFLAAFLISGNIVMAIIGAIISLLLSLLFVIVAGRLTGTIGTSNLPVSG
                     MTIASLVIVTLVFVIMGWTDLEANKSLLLFGSFIVVAIAIAGGYTQSQKVTYIIGGSK
                     NEMQRYFTIASIVGVIVVVGVILLLSDQLRATGDNVQFALPQANLMSTLTSGIMSGSL
                     PWVMIIVGVFMAIVLYALNLPIMTIAIGFYLPIATTSIILVGALIRLFVELVSKTEKE
                     KEVKVSNGISLSSGLVAGGSIIGLIGIILQVTGVVTPKVPSGFAATNSMAIALLVVLV
                     VLTALPIVLSKVKNNEQE"
BASE COUNT        10636 a         3863 c         3680 g         9847 t
ORIGIN      
        1 tttaaagtat cttatggagg aataccatgc aaaatcataa ttatataaaa atgttaaata
       61 aaaaggtaga agtattatct aaggaattag atagtagaaa ttttaaatta aaaaccttaa
      121 atgaaataga ttttttttct aaaataccaa acagagaatt tatgtaccaa catattgatg
      181 actgtatttc gaaaaatata catataggaa tcatgttatt aaatatcgat aactttaaat
      241 tatttaatga tatatatgga tacctagtcg gagaaaattt attaattact ctaactcaaa
      301 acctattgca ctttattcca gaatcaaagg gaatagtcgc ttatttaaaa ggagaaggtt
      361 ttttaattgt acttccaaat gtatcaaata aagagttaaa taatatgggt gcaactatta
      421 tagagagcgc ccgagaatta aaaataagac ttcctaatga aaaagaaata tactccaatg
      481 ttacatttag tatcggcagc actatctgga atggtgaacc aaacatgact actttgatgc
      541 ttttcaacca agtgtatagt gctttaaaaa aggctaaaag tgaaggtaaa aatagataca
      601 ttctatttga ttttaaagat aatagttttt acaataaatt agaaaatgat aaggagttct
      661 tatcagaaat atcacatgtt ctacaaacta aacaaattga agtattttac caaccacttt
      721 ataatataaa aaataatact atagttggat gtgaggcact tgcaagatgg agacacccta
      781 ttaaaggact tcttactcca gataaattta ttcctttatt tgaacagttt ggtctgataa
      841 cggaattaga tttgcattta tttgaagaga actgtaaaaa tataagaaga tggattgata
      901 acaatgatgt ttttgttcca atttgttgta acttttctcg tcttcatttt tttaacccta
      961 attttccaag tatgctaaaa ggtattactg aaaagtacag cgtctctaca tcaaatttta
     1021 gcattgaaat aacagaaaat atattaatag aaaatacaaa tacaattatt caacaactag
     1081 ctgaacttcg ttcttatggt ttttctgtat caatggacga ttttggttct ggatattcat
     1141 cttttggtat gttgcaaaat ctaccttttg atataatcaa aattgatagg atatttttat
     1201 ctcataattt aaatgatttt aaaaatacaa ccattttgta ctctataata aacattgcaa
     1261 gagctcttgg gatgcttaca gtatgtgaag gtgttgaaac agaacaacat gtagaattta
     1321 ttaaaagtat aaattgtgat atcgcccaag gttattatta tgcaaaaccc atggagtgca
     1381 aagaatttga aaaattacta tctaaagaga ataattcaga gaaaatgttt ataaaatatt
     1441 ctgatatagg aaaatcttta atagagaaga tatttgatgg attttttatt atgcaagata
     1501 tgaaaaaact aagagctaat gtcactgaag acattcaatg gtttgatttt tacaacaaaa
     1561 ctgaaattac atcttttcct aaagtaatag agcattttga aaaacatatt aaaggtaaaa
     1621 aattttctat tctttacaaa agtataaccc cacataatac tgaatcagat tcattattaa
     1681 ttagtggaga agccgtttta tcagatgaag cttctgaaca tcaaaaatat agcaatttct
     1741 actttagtgc aaattgtata atttcagaaa ataaattact cttagcaaaa ttacatttgg
     1801 atttaattaa aactgctggc tatataaatg aatttgaatc atattcaata cctaaaatga
     1861 caaatacaca tcttgatgaa agtaatattt tatcacaatt ttattcaatg ttgcctgtag
     1921 gtataattcg ctacgattta tttggtgata tgattattac ttatatgaat gaagaaatgt
     1981 ttgatattat aggttataca aaagaacagt tttttggtga gatgggcgga aatttaagag
     2041 caattgttca tccagatgat ttagactttg tttataaaaa aagtttggac atgattgaca
     2101 caaataaaat tgaaccattt ttatatagat tcataaaacg agatggtcaa attgtcacag
     2161 ttatgtatac acaatgtaac ctttctgcaa ttgatggaag gtcgattgcc cagggtatgt
     2221 atgtagatgt aaataaaata aaaaatttaa caaaaatcta gtacaaagta ttttattttg
     2281 gagaaattaa tatgtttaaa gaaatttttt taagaacttt tccaggattt ctaattataa
     2341 gagattctaa ctacagaatt atatttataa atgataattt aaaaaattta attaaaagtt
     2401 atatgcaaga taatccactt ggaatgacta atatagaaat agctaagaaa ctccctgata
     2461 atattgctaa attttttacg gattctcaca atattcaatt agattgggaa aaaaattatc
     2521 cttatgataa aatttcaaat tggattttag agtttaaaaa agatactaca agctattgga
     2581 atgttttaga atacaaagta gatgttgatg aaaaaaccta cataattact atggccaatg
     2641 atataacaaa actctatgaa gaaaataaga gaaatcttca ctactcaatt actgacccat
     2701 taactggagc atataataga aaatatttaa atgatagatt tgacatattt ataggagatt
     2761 atattgtact aattgactta gataatttca aaatgataaa tgactatgaa ggtcataatg
     2821 taggagacaa gattttatgt gattttgttt cacttttaaa taaagaatta ataaattcta
     2881 cttctattat acgattagga ggagatgaat ttattgttat attctctagt gatgttgaca
     2941 aaaattatgt ttattcacag ctagaagcct taagagaaaa ttttttaaaa gtattctcta
     3001 agtataagta tctttcattt agttatggtg ttgatacagt aaaaagaaat ttaaaattga
     3061 caatagttga attggataaa aaaatgtata aaaacaagga gaaaaacaag aaaaaatttg
     3121 ataaaaatga ttattaacat tgttatttta aaataaatta aaattatctt tcttttttta
     3181 aacttgtaca ttagtttata aatatgtatc tatacaagat attgacatta agtactgaag
     3241 catatattgt ttcagttctt ttttgttaaa attaacttat ttaacaaaga tatcatagtt
     3301 taacaaagct tttaatttaa atatattgat gtataatata attaaaatat aatagcaaaa
     3361 agaaaggagg aatatcatga gcatactcct aaaaaaagca cccaaattag caaaacatat
     3421 tataacaagt ttttatataa atcgagatat caatgaagta ttaaaatacc tttgtgaaaa
     3481 tgttacatgg attggaccag gagaacagga atttctcact tcttttaatg aaataaaaaa
     3541 ttatttttat gcaggtcaag atgaaattcc ttcttgtgat ataaataatg atatatttga
     3601 aactgtaagt gaagatgaga atagatgtat ggtattagga agatatactg ttcgaactaa
     3661 agaaaatgct caaatgattt tagaggtaaa tcaacgttgt acatttgaga taattgagga
     3721 tagaggaaaa ttattaataa agcacatgca catatcaaac ccttatggtg aaatgcaact
     3781 tgatgaatac tttcccacta aaattggaac tcaaagttat gattatttgc aaagattact
     3841 aaaagaaaaa acagaagtta ttgaaatgat tacaaataat atcaatggtg gcttaaaagg
     3901 tagcaatgat gatagtacat actcattttt ctatgtcaat gagggattgc ccaaaatatt
     3961 aggatacaca tatgatgagt tcatggaaat gagtggtggt tcagcagtgg gtgctgtata
     4021 tccaccagac ttacctaaag cactagaaga ttgtgaacga tgttttgcaa aaggacctac
     4081 atattcatct gaatatagaa tccgaaaaaa agatggaaca ttaatgtggg tattagattc
     4141 tggaatgaaa tcgttaaata gtgatggcat agtaaaaatt aatagtatta ttactgacat
     4201 aacccaacta aaaaacatag aatctgaact aaaattagaa agagagcgat atcgtattgc
     4261 tttgcaaaat attacagata tcatgtttga gtatgatatt gaaaacgata cccttataaa
     4321 atatcaacgt gtagaaattg acaaaaaaat tgaattagaa aattttgaaa caaaaaatta
     4381 ctccaaacta ttagaatctg gtaaaattat tcaccttgat gatattggaa aactactaga
     4441 agtacttcat ggaaatcttc gtgaaacaat tgagataaga caagtaaatt cattaactaa
     4501 aaatgaatgg agatggattc gtgttcaatg ttcagttata tatgatagtg accataaccc
     4561 tatcaaaaca ataggcgttt taaaagacat aactgaagat aaatctaaat tagaaagttt
     4621 aattaatcag gctcaacgtg acccactaac acaattatat aatcaaagag tatcacaaaa
     4681 tcttattgaa gagtatctat gtagttctga ttccaaaaat aatgatgctc tattagttat
     4741 tgatattgat gattttaaaa ctgtaaatga tacttttggg catctagagg gaaatgaagt
     4801 tttagttgct gtatcaaaaa tactattaca taacacacat gataaagaca tagttgctcg
     4861 aattggtgga gatgaattta caatatttat aaaatcatta acaaaggatt taataataaa
     4921 aattactaat gatattttaa atgatgcgag taaaataaaa gtaaaggaca atcataaaat
     4981 aacattaagt attggtattg ccttcactga tgattcaact aaattatata aagatttatt
     5041 ttctaaagct gataaagctc tttatctatc aaaagcagat ggcaaaaatt gttatagtac
     5101 atacgaataa taaagctatt tatacgacca agaatgaacc tttaaattta atttcatagt
     5161 ctaacaatca aagagttgaa gctaatctta tagctagtca actagttcct agaaaaaatt
     5221 taaacaatat atttattgat actgctcatt tttctaaaga gcaaatttat ataaaaaatt
     5281 aaacttttat atacatacag atactaatga taaacagtta tcattagtat cttctatctt
     5341 aaaacaaaat gataatttat ctatataaaa aagtgctttg ttgacagagt tcactttttt
     5401 atataggtac agaatttaag tataaataca tgttttattc tatacatttt agttaatgta
     5461 aaatttcttg attgtactct ctaacttttc aacattatac tatttatata ccacttagtt
     5521 aatataaaac tataaaagtg ttgcaattac tgggttttat aaagttaatt tacataccac
     5581 ttagttaata taaaacttta gcaccttatt tatctataga gctacagaaa gtatttacat
     5641 accacttagt taatataaaa catgtattta tttgttcttg tactattgtt ttactctatt
     5701 tacataccac ttagttaata taaaacaata tcaaaacaaa taaaaatttt taataaatca
     5761 ttatttacat accacttagc taatataaaa cattctggta ctgttgctat ttatgttccg
     5821 tattcagaat ttacatacca cttagttaat ataaaacttt ggagttgtta cagattattt
     5881 gccttatctc tctatttaca taccacttag ttaatataaa acccccaaat aaactgggca
     5941 ttttcaatac ttacacatat gcaactctct taaatttgca gtgaaccatg agtagtgcaa
     6001 atgataaagc ttatcatata ccctcaacac cttatatttc aagcgttaag ctctactttg
     6061 taacaaatat cgctcactgc aaaactattt acatttttat tatatcataa atatattatt
     6121 tttgaatatc tataccaatt tgtggtataa taaaagcaag aagaactaca atctatttag
     6181 acgtagagtg aagttcaaat actaatgttt attctttttg aacttgattt ttagttcaaa
     6241 actaaagtca ctcctgcctg tgtgaactat acccgctgtt ttatacagtt aaaacatgta
     6301 aaaatagtta taaagtacta gtgtaactca ttagtactct ttttattata ttcatataat
     6361 taatatctat attttaaagc ttactatcta aaaaagagca cgtcaaataa aaccctagat
     6421 atttaatttt tttagagata cttaaaataa attatgctct ctctgtactt aaatatttcc
     6481 taaattcaaa tggagacata gaatttaatc tattttgata tctaaaatta ttatagtagt
     6541 atatataatc atctattgct ttaactaatt attcatatgt atcaaatttc cttaaatagt
     6601 acatttctac ctttaaaatt ccccaaaatc cttccatagg agcattgtca aggcaacgac
     6661 caactcttgt ataatatatg tatatctaac accattaaga aatatatatt ttataatata
     6721 tatttcttca tttaatgcac ttagaaaagc aaagaaaata cagattagta cactttttag
     6781 agaaaaataa aattaataca tctaaaatat taaaaaatac tttagataat ctaaagacac
     6841 atctcaaagt ttttaactaa attataaaac taattacaaa tgctatgtaa cgataataaa
     6901 taaaaaattt acttcgctta agcgacgtgt cgttgaaata tttcatgttg ccaactgatt
     6961 cataagtcga taagaaaaga acactaatta tttagagttc ttttcttagc tacaagtgga
     7021 ttataatact tttaaatctg tgagaaaatc atatataata atgatatgaa caagtaaaga
     7081 aaatacaaaa atgacaaaat tataaataaa atattataaa atcataaggt cataattgtt
     7141 atattgtaga aacaaagtaa agagagaggg tggagaataa atgaatcaaa aaaaaataaa
     7201 gattagtatt aaaatggtaa ttataggtat attttctgta attattgctt ttgtattatc
     7261 cagattattc gttgagtata ttgatgaatt attaaggtct aattggcaat atcagctatt
     7321 tgaaagtgag agttcatata taatatttgc caattgtatt atgttctcga atatattatt
     7381 agaaatattt ttaatatata tttgtagaaa atttagaaaa atctaatatg gtcaacgtat
     7441 ttcgaatcga atacttcatg attatttagt ctattgtgct agttaaagtt taattcccat
     7501 ataaagtaaa ttgattttag taaaacaaat cacccctaag ggttagttta tggataagtc
     7561 atccaaatat taactcttta atttttgcta tagattcatc tttgctcata actttactta
     7621 taaaaaatga aatattataa gctagaactt ttattctaat tctagtcata aatcctagca
     7681 tagagttact ttttttatat tataaataaa actatacaac aaagataata tttagtaaaa
     7741 ataactttca ttttaactaa acttaaaatt caattactct ctaaattgac gtttaggaat
     7801 tagttcaaaa taaaaaagag tgtatatatt gaaattacta catttcaata tatatactct
     7861 ttttaaattt ctcagttttc catggaactt tactttattt atttctgtat tagttgtatt
     7921 ttttatgcat ttttctttaa aatcatattt tctgattcaa ctcttatatt ataaaatttc
     7981 tttactaaat tattctcttt ttctatatct tcatatatat tttttaatcg ccttttacct
     8041 aatcttttat ctaacattac taatgattta actaatatat tatatgattt tatcgcatct
     8101 tctattgaga tatttaaaca tatatttact gcttcataaa aatcaaattc attaaaaaat
     8161 ccatttttat ttacaatctt atatcccttt gcatctcttt ctaaatactc ttcctaaata
     8221 ctcttcaaaa ctttcatcat ctaatctaaa tcctatatca ctatgaaaaa taggttttgc
     8281 atttggatat agttctattg ttttatcaaa agtttcgaat accaagttat tattattcga
     8341 ttttccaata gtccactata aacaataata agtttgttgc tccaaataat gtaacacttg
     8401 tagaaagatg ttacagatat ataaaagata gagaatgtag tttttctcct aatactatta
     8461 gaaatagaaa aaatatgatt aaaaatcaaa ttgaagtttt ctttaaagat atgaaattaa
     8521 tagatataac accttctatt ttacagtctt atataaataa catatacaat gagcacatgt
     8581 taaattcaat aaaaaaccaa gtcgatttta taaaatcagt attgaaagaa tcttatagat
     8641 taaaagaaat atcagaaaat atttgtgatt ttgttacaac cccaatcaaa aaagtagttc
     8701 aacttctaag ctttatacaa aacaaaaagc tcaattatta ttagaaaaat caaaaaatat
     8761 tcctataggt ataccaattt ttttgatgct aactcttgga ttacgttttg gtgaagctgt
     8821 atcacttata tggtcagatg tagaccttga taaaaaatct cacatgtaaa tcaaacatta
     8881 gtttatgtat acaataaaat aatcttcaaa gaccctaaaa cccctatatc aaaaagaaaa
     8941 ttatttgctc ctgatgaact tataagtttg cttaaagggg aaaaattaaa acaaaataag
     9001 ttaaaattac agggtatatt gaaaaatgaa tttgatttga tatgcttaaa taaaagattc
     9061 aacccgtgag ttcaaccaac ttttgttgtt cattttaaaa aactattaag agataataat
     9121 cctagagata taaaagtaca tgaattaata catgcaaatg caactttgat attactttca
     9181 ggtactaaca taaaagctat ttataaaata ttagtacatg cagatataaa aatagctatg
     9241 aatatatatt ccatattctc acttattaga tgaaatgaat aaagaaacat ctgaaaatat
     9301 aagcaaaata ctattcaaat aatgttcagt tttatgtcaa ttaaatgtca ctcaaagtgc
     9361 ttttttttca tttgtcagtt aaatgtcact taaagtacct ttttcctatt tgtcagttaa
     9421 atgtcagtaa tatataaata agatatattt ataagaccct taaaatcact attatttaat
     9481 aaaatttata attatatact atttgctgtt gacattggta ttataccata gtttatcatt
     9541 tatattagaa ataaaattat taattaatag tttcgtaatt ttataataga tttacaggga
     9601 gtgatagtaa atggatacaa aaatatctat aaacgaaaac atgagtctag gtaaaagatt
     9661 ttttaaatac ctagcaccat ctgtagttgc aatgtgggtt ttctcactat atacaatggt
     9721 tgatggaata tttgtaagta aaggagttgg agaattagct ttagctgctg tcaatatatc
     9781 aatgcctttt attaatttta tatttgcagt ttctctatta ttttcaactg gtgcatctac
     9841 cataattgca atctatctgg ggaaaaaaga tattaaatct gctaacgaag tcttttcatt
     9901 taacttagta tcaataatta tattatcaat aattatatta gctataactt tttttaattt
     9961 agatagatta gcactatttt taggagctac agaatctaca attggtatgg ttaaagatta
    10021 tttaggaata ataatatttt ttaacggatt ctttatagtg tcttattctt tagaagttat
    10081 aataaaaact gatggctttc caatattagc tactgttggt gtaataatat ctgctcttac
    10141 aaacatcata cttgattact tgtttgtaat tgaatttgga tggggtgtaa aaggtgctgg
    10201 tattgcaact ggtttgtctc aagtcttttc tactattttc ttcttaatac attttttaag
    10261 aaaaaactct actttgaatt tttcaaaatt tagaattgat tttaagacac ttagaaaaat
    10321 tgtatttata ggttttcctg attcaactac tgaattaagt tgtggtatag ttgttttact
    10381 ttttaactta agtcttacta aatatattgg agaaaatgct cttatttatt atagtgtcat
    10441 aaattatata aatacattag tacttatgac tatgatgggt ataactcaag gtatgcaacc
    10501 acttactagc ttttattatg gtgcaggaaa tatagataat gtaaaaaaat tattaaaaat
    10561 ggggataaaa gccacaataa tagcatcagt tgctgtattt gcaatttgta tggctttttc
    10621 aggaccaata gtctctttat ttatacatcc agaagaaaca atgctattca atgaaggagt
    10681 tcgtgtattt aaaatattct ctatatcttt cttattggta ggaattaatg taataatttc
    10741 tggattcttt gtatcagtag aaaaaccatc aatttctact gttatatctc ttggtagagg
    10801 gcttgtaata gttgttttaa gtctaatttc aatgatacta atatttggtg gacaaggaat
    10861 atggatgact actattgttt ctgagtttat atgtcttatt ttatcattag tttttctaaa
    10921 aaagaatttt tctacattag actctaatct aaataaggtt gcttagatga taagagtgac
    10981 aatatatgaa aatatctaaa taaaaaatac ctattataca atcttgcaaa tattaaacct
    11041 ttgcaaaaat taggattata taataggtat tttatttatg taattatttt atttctaaat
    11101 atccatagcc atcttttatt aaagttcctt ttacttcata ttctacaggt tttttaaata
    11161 ttggaccatc tacgacaaat gtatgatact ctccatgttc tccacatggg tctgcaccag
    11221 cattttttat cttctccaca agttctctag ttaatttttt tcctaaaaat tcctctccta
    11281 agtgctttaa gtttactttt gttacaacac tacaaaatcc actgtctata aattcatata
    11341 ctaactcctc tctactctct tgccataatg gtaaatctac tttcatacca actgcttcac
    11401 atctatcagt atcccatttt ctatgacttt ctatatctat atctccatat gcacatatag
    11461 ttgctcccat gtttttagct tttattaaag ccctctcaaa gctctcttca tatccttcta
    11521 caccacactc agcaactaat aaaggtaatc ctatattgct acttacttgc ttaagcatat
    11581 tatattctaa attatgtgtc catgactttc catgttcttc acttatagtt gtcaagagtg
    11641 ccacaggctt atatcctttt ttcaacattc gatttaatgc taatatacta tccttaccac
    11701 cactaaatga cattacaaat ttttcatcag ccaattttaa cacctctttc atctaacttt
    11761 gttttattaa cataattata ccaaattttt gagttgcata gcaaattata ttaaattgtt
    11821 aattcactat aaagaatgtc cctaatatta aaagtataac ccctatgtat ttgtataaat
    11881 gcattgtttc ttgtcccaaa attacgctat caataaaaaa tccaactaaa acttgggata
    11941 caagagttaa tataagtgta tttgatactc ctaaatttgg aattgccttt actgtaaaat
    12001 atattattag acctccaaat actcctccta ataacaactt ataattaaaa ccatttatag
    12061 atactagaca ctttaagtca cctgtaacta ttatacttgc caaaaaaaat attgctccta
    12121 cagttaagct tataaaggtg gccactaatg ctgttgtact ttttcctagc tctccattta
    12181 taaaagcttc aagagctgta gaaccacctg ctaataatgc aaatattatt gcccataatt
    12241 ttgtcatgga caaacctcct tctatataat tctattaata gaagtttatg atacaaccta
    12301 ttaattaatt actaagtata gtttaatttt tatacactag gctttatttg gatatgaatt
    12361 tctgttataa ttgttctatc gattttggag gtgattttat gtacacaaat tttaatatcg
    12421 attttaataa ctttaataaa aaagaaaatg ccattaaatt tatgttaatg ggagtattac
    12481 tcattatatt aggattatta tgccttacat ttaagacatt aggtataaaa ttaatttctt
    12541 ggacttttgg tattgcactt ttattctttg catatttaaa tttaaaaaat attaatgaac
    12601 taaaaagata tgctactaaa gaagaaatta aaccctctat taatatccaa tgggttctaa
    12661 taattgcatg tatattactt tttgtatttc ctcaaaaaat acaaagcata ttttcattat
    12721 ttttaggatt ttatttaatc tttaatcaac tagtagcact tgtaaatagt aagaataatc
    12781 cttattctaa atttacaact tggaatatag ttaagattct atttggtata tgccttatat
    12841 tatctccact ttttttatct agatttatag tatcgataat gtcttttttc ataatattat
    12901 ttggactagt tctatttttc tctggaaata cagcgagaaa atattaatta ttttctcgct
    12961 gtatttttat attacttaaa atagaatttg tttaagttat ctatttgtgg taatatgttt
    13021 tttgtacaag ctcagcgtac aattttatta gaaataggtg attttttgga taaatttaaa
    13081 tatgcttttg ataataaaag atatcatact tggaattact atcttagaaa tacttttgga
    13141 gaaaaagttt ttaaggtatc tataaatgca ggatttactt gcccaaacat tgatggaagt
    13201 cttggttatg gcggatgtac ctattgtagc aaagaaggct caggtgattt tgctggaaac
    13261 cctaaagata atcttatcag tcaattttat aatataaaag aaatgatgtt aaaaaaatgg
    13321 cctcatgcaa agtatattgg ctactttcaa gcctatacaa atacgtatgc tcctcttgaa
    13381 gttttaaaag aaaaatatga gactatatta gaacttgaag atgtcatagg tctttcaata
    13441 tccacaagac ctgactgttt gccagatgat gttgtagaat acttgtctga actaaataaa
    13501 agaaccaatt tatgggttga acttggactt caaacaattc atgattctac atctaaaatt
    13561 ataaatagag gtcatgatta taaaacattt gttgaaggtg ttgaaaaatt aaagtctaaa
    13621 aatataaagg ttgtcgtaca cattataaat ggtcttcctg gtgaagatta taatatgatg
    13681 atggaaacag caaaggcagt tggtaaaatg ggtgtagacg gtataaaaat ccatctattg
    13741 catgttataa aagatactcc tatggaaaag atgttacaaa atggtatgtt aactcttatg
    13801 gaacaagatg aatatatcaa tcttgtctgt gaccaattag aaatacttcc tgaaactatg
    13861 ataatccata gactcactgg tgatggtaag agggatgaac tagtaggtcc tatatggagc
    13921 ctaaaaaaat gggaaatttt aaataaaatt gatgatactt taaaggctag aaattcttat
    13981 cagggctgta agtttgttta atacaaaata ataactatag aaatggttat acaaatatag
    14041 tcctttctat agttatttag tatttacaaa acaaaatatt aatttgtaca tttatacaat
    14101 atataataaa aaatagatag tctaaactgt atctacttat attttatttg tactgtccac
    14161 tttattctat aaaatttata aatagttatt ttttaatacc aaatataatt tggtataatt
    14221 tttgttggaa tctacaatgt ataccaataa atccttttta agtatataat tctaaatact
    14281 ttcagttttt tcaatctgct ttaacttgtt ggacttataa aatattctta tttcatcata
    14341 agaaagatta agtgctacta ctgcagatat taacatcaac atcactccaa ataacttccc
    14401 taaggaaaaa ctttctccta atatagtgca accaattatt actgaaccaa ctaattctac
    14461 tgatgccaca acacctgcaa ctgacaactc tacaccttta gatatacctg ctgcataaaa
    14521 aatatatgca agtgcagctg gaacaatgcc taacattagc atacaagata aaacatctaa
    14581 atccttagca taatttaaga tttcccaagg tcttgaagaa ggaatcataa atatagctcc
    14641 aaataagaaa ctgtatacta atattgttga acttgaaaat tcctttaaag catttttact
    14701 aatagttggc ataagtgcat atgctattgc tgataatact ccaagtaaaa gcccaaaagc
    14761 gtttaaacct tgaaaatcca aacgtccacc agtcactgcc ataattgcac caataaaaca
    14821 aagaataagt gaaaataatt tgcttctagt aatattttct ttataacata attttgaaaa
    14881 tattgctaaa aacagtggtg atgtatatag taaaactgct gcaattgata cacccgcaat
    14941 gtcaattgct ttaaaataac ataaattaaa cattgcctga catataattc ctattattac
    15001 tgaatatata attccttttt tgtttatctt taagatttgt ggtgtcctta tagatgaata
    15061 tacgcctaaa actataaatc caaaaaacaa tcttgtaaat gctacttgtt ctgatgtcag
    15121 tccatttccc attaaaaact ttccaaataa tccaagtgta gcccataata accctgctat
    15181 tgctataaat acataccctt tacttttttc tcccatttta aattcccttc tatttttatt
    15241 tagtcaattt acactcttct ttttaagcaa aaactatgcc atcttaaatc aatatatatc
    15301 gactctaaaa ccctagtctt tccaataata ctaataaatt ttcaattttt ttatatttat
    15361 ttttcgctta ctttaagttg atttagactt attttaattg taaaatgagt tatatataac
    15421 ttatttaaag ttaaaataga ctgaaactta tatataaata tcaaatttta agtatattaa
    15481 tcaattaaac ctattttaat atatcattaa aaatttagga ggaaaatata aaaatgaatt
    15541 tagatttgga attttatcaa aaaatattag aagcttccca tgatgaaata tgtgtttctg
    15601 atgataaagg aataataata tattgtaata aagcatttga agaaaattat ggtctaaaaa
    15661 aagaagatat tttgggaaaa aatgtttctt ttttagaaga ctctggatat tcaacaaaaa
    15721 gccccatacc aattgtatta aaaacaaaga ctaaattttc actggaacaa gatactcaaa
    15781 cagggaaaaa gcttatcata actgctaccc ctatatttga tgaaaatgga cacttagaat
    15841 ttactgttga aaattgtaga gatataactg aacttaataa tataaaaaat aaacttgaag
    15901 acacaaaaaa gcaagtaaaa aaatataaat ttgaagtaga aacactatac agaactgcac
    15961 ttagaattga agatactgta attatggacg gaatagttat gagaccaatc ataaacacag
    16021 taaatcatgt ttctaaaacg gatgtaagtg tacttttatt gggtgaatct ggaacaggaa
    16081 aaagctcact tgctagatat atacaccaca acagcaatag atctaatggc ccatttataa
    16141 caataaattg tgcaactata tctccacaac ttcttgaatc tgaacttttt ggatatactt
    16201 caggtgcttt tacaggtgca agtactaaag gtaaagttgg gctcgtagaa ttagctaatg
    16261 gaggaactct ttttttagat gaaataggag atataccaca aaatctacag gcaaaatttt
    16321 tgcaattaat acaggataga acttttactc cagttgggag cttaaaaaat aaaaaggtcg
    16381 atataagaat tatatctgct acaaatgttg atttagtttc aaaagtaaaa gaaaaaaagt
    16441 ttagagaaga tttatactat agattaaatg ttatagaaat aaaattgcct cctcttagag
    16501 aaagaaggga taatctagtg gaaattataa aatattattt taataggtac tcaagtgact
    16561 tcaacctaaa taaaactatt tctaaggaag ctatggatgc tattgcaaat tatagattcc
    16621 caggcaatat aagagaatta caaaatataa ttcaaaaaat cttgcttact tgtacagata
    16681 atcatataac aatagatgac ttaccaaaca ttcttacaaa aaatatacat attaccaata
    16741 atggaaataa aacacatata tcccaaatta ataaggttat tatatctgac tcaaaatcta
    16801 ctaattataa aaataaaaat tttgacactt taattaaaga gtatgaaaaa aatataattt
    16861 tggatgctta tgaaaaattt ggtagttctt ataaagtagc caaacacctt gaaatctcac
    16921 agtctaaagc caataggtta attagaaagt atactaatac ttaattttaa atttgataaa
    16981 taaaaactta acactattta catattgtaa atttggccat ttactgttat tatgaatatg
    17041 gttgaaatta tttaaataaa tactgttgac ttagtttcat atatttacca ttaaactttt
    17101 atcataaata tcatatcata ataatatatt taataagtct aataaactat acgaggtgta
    17161 aatatgttaa tgaataccca ttttattatg gcaaaatcaa tacttgataa tatagatgaa
    17221 aataaaacat tctttataag cgaaaaaaat tttatatatg gcaatataaa gcctgatgct
    17281 ttttctaaat ataaattaaa aaaacattat ttagatgagt catttaatat gattgtttct
    17341 aaaataaact atctatgcag tcttaatata gatagtcttt ctaaatactt ttcagtaagt
    17401 agacttagtc aggaactagg agtcatatgt cattttttat gtgatttttt ctgtgtagct
    17461 cacagtgaac gttgggaatt taagcatagc ttaaataaac atgttactta tgaaagagaa
    17521 cttggagcat ttgcaaaaga tgttgattta tctaaaatta aatgtggtac tataaatagt
    17581 agttttgaga agttttttac taaactttat agtgattaca aaaagaaaac tgattatatg
    17641 aatgatttaa aattttcagc ttatacttgt aatagtgtca taaattatat acttgactct
    17701 attttatcta atacaataaa atcatatcat atagcaaatt gcggttaact taagaatttt
    17761 taaacttata taaacttata attgaatata attaaaatga ctgctctata gaatataatt
    17821 ttatacttct ctaggacagt cattttttat actaactatt tatctccatt ataacatcta
    17881 tggcactttt tacactatct actacataat ttgcactttg ctttacatct tcccttgcct
    17941 tagacattgc aaaactatat ttaaatcctt ctaacatgca aatatcattt ggagaatccc
    18001 caatagtagc aacttcatct ggacttacat ttaacgtttt tattacatgc tctatacttc
    18061 ctctttttga cacactttta ggaactatat ttaaagtata tgttccagaa aaatatattt
    18121 ctaagacttc actaaactca ctttttagat aaagttctat tctggataac tcttccaaat
    18181 tttcagaaca tacgcctata tttcctataa gattatcaaa actacttctt ttttcaaaat
    18241 ctgggtcaac tataaagtca ctagaataat gtctaaaagc agtggtatca ctacccacaa
    18301 ttacatcacc cttatattta aaataaagtt gtgcatcact tgactcaaat ctatcaatga
    18361 ctttttttac taaattatca tctatcgttc ttttaaaaat tacattataa tctttatcat
    18421 atataaatga accattttca catacatagt atccattcat atctaaacta tctttgataa
    18481 tattatgtgc ttgtttaaaa attctacctg tagcaacatt aaactgtact cccttttttt
    18541 ctatttcttc aattgctact atatcttctt ttgttatggt tccattttca tatagagttc
    18601 catctaaatc acaaaatata tgttttatca taaattaaaa gcctccaaaa acaattttat
    18661 attttactaa tactttaatc aaaataactt aaagcttttc taattgtatt ctcaattcat
    18721 taagcaaatc tctagtagct cttaatctta tctttcttct atccccatta aatatatttt
    18781 tctttactat tgtttttcca tttatatata ttgcagtata aaccagtccc acaggcttct
    18841 ctttggttcc tccaccaggt ccagctactc cagttgtaga tatacctata ttagctttaa
    18901 gtcctttagc aatgccctca gccatttcta tagctgtttg ctcacttact gctccaaact
    18961 cttccaaagt ttctcttttt actcctaatc ttttcatttt agaatcatta gaatatgtta
    19021 cacaaccttc aagaaataca gaggatatac ctggatagtt tataagagat gaagaaacca
    19081 aacctccagt acaagattca gccactgcta ttgttagatt tttttctaca agtagcttag
    19141 aaacagcatc ttctaaagca gtctcccctt cagatatgga aatatctcct tcagtatata
    19201 catatttacc aactctactt tttatttttt cttctactgg ttttattaat ttaaaagctt
    19261 ctctttcatt ttcagccttt gcagttattc ttattgtaac ttctaattct tttgcatata
    19321 aagcaactgt tggattagtc tgctcattaa ttatatcgag aatttctaat tctagatttg
    19381 actcacctat tccatataat cttaaagtct tagatactag catttcattt gtgaattgtt
    19441 gtaagtaagg tttgacactc tcattaaaca tagctttcat ttctcttgga ggtccaggca
    19501 atactattat aaatttacca tcttttttta aaattgcccc tggagctgta ccattattat
    19561 tttttagtac tatagcatct tttggaaagt acacttgttt catattattt tcggccattt
    19621 ttttccctgt tttaacaaag aaagattcca aaacctctag tgaaggttta tgtagttcta
    19681 atttctgacc aaagtattct gcagcagttt cttttgtcat atcatcacca gttggcccaa
    19741 gaccacctgt agtaattaca aaatcacttc tctttaagct ttcatcaaaa cattctaaaa
    19801 gtctttgttt gttatctcca acagtactct gatggtatac ttctattccc aatgatgcaa
    19861 gttctttagc taaaaattga gaatttgtat ttactatatc tcccaataat atttctgttc
    19921 ctacacttat tatttcagct ttcaaaattt atcactcctt gatttatagc cattaagact
    19981 agtcttaagt tcataacaat aattttagca aaaataataa cataaataaa gttaaaaaat
    20041 catttaaaca tatctttaaa ctagattatt attctaaaac cacacataat ataaacttcg
    20101 tatatagtgg tactttcatc ctcaactgtt aattcatata attaagtatg taaataaaaa
    20161 gtttacttta aaataataaa aattaaaata ggagtgtata atatgaaaat agcagttata
    20221 atgggtggaa tatcatcaga gagagaagtt tctttaaatt caggaaaaga gatatataat
    20281 aaccttgata aaaataaata tgaagttgta aaggtaataa tagatgataa aaaagatata
    20341 tttacaaaga taccagaaga tatagatttt gcaatacttg ctttacatgg aaaatttggt
    20401 gaagatggat gtatacaatc aatacttgag actatggata taccatactc tggatgtgga
    20461 cctctttgca gtgggatgtg tatggataaa aatataacta aaaaaatgct tagagattca
    20521 aacctaccta ctgctccatg ggttttagta aaatcagttg atgaaataga ctatgatgaa
    20581 atagataata taggttatcc agtatttata aaaccaaact caggaggttc aagtgtagcc
    20641 acattcttta ttcattctaa ggatgaagtt gaagaagcag ttagaaaagg tctggaagtt
    20701 gatgaatttg taatgattga aaaatatata ccaggtggag aatatacttc attcatatta
    20761 aatggagaag tgttccctac aatatctata aaatctgatt caggattctt tgattatgaa
    20821 gctaaatact cagttgaaaa aggtgcaaag gaagaagttg tttacttaga tgaagaactt
    20881 caaaaaagag taaacgaaat ttctgagact tgttggaaaa tttttaattg caaagcatat
    20941 gtaagagttg atatgattat aagtgaaggt attccatatg ttcttgaact aaatactctt
    21001 cctggaatga ctcaaactag tttaattcct agaagtgctg ctgctagagg tattaagtac
    21061 agtgaactat tagataaact tatagaatac tctttaaatt agatgaaata atttgaggtg
    21121 gtaatttgat attttcaaat ctaaaattaa atgataatga accaatttat atacaactaa
    21181 aaaattatat aagtgatatg atatcaaaag gactaattcc agacaatagt aagctccctt
    21241 ctacaaggga gcttagtcag cttttacaag ttagccgaaa ttctgtagtt ttaacttatg
    21301 aagaattaaa atcagaagga ttaatctact ctatttcagg aaaaggtaca tttgttaaat
    21361 caaagaataa gtcttcaaat actacatggt cattaaactg ggattgttta gagaatacat
    21421 actctaaaaa agccaatgaa ttggacatta taaaaagtga aattccttgg agctctgacc
    21481 tgatttcctt taaaagtatt tctccagatg gagatttatt cgacatggaa gaattaaaaa
    21541 aatccttttt aaatagaatt tcactagaag gacataaatt actcaactat ggatatgcac
    21601 aaggttataa gccacttatt gactatttac tagaatatat gacaaacaaa ggtgctgata
    21661 taagtaataa agatatacta ataacaaatg gattcacaga aggctttgat attattatgt
    21721 catcttttac ccaagaaaaa gactacataa tatgtgaaaa tcctactcat aatactgcta
    21781 taaaaattat gaaatctcat aacctcaata tcttaggtgt agatataaat gaagatggtt
    21841 tggacttcaa tatgctagaa gacaatttag ttaagtataa aaataaaata aagtttgcat
    21901 acattacacc ctcttatcac aatcctacag gaattgtaat gactcctgaa aataggtaca
    21961 aattctataa tcttatgaaa aaatacaata tagcaattat agaagatgga tttaatgaag
    22021 aattactcta caatagttct catatatttc ctatctgttc cctagataat atgaataatg
    22081 gggtcgtata tattggtagt ttttcaaaaa ttttatttcc tggaatgaga attggatgga
    22141 tatttgcaga taaaaaagta atcaataagc tagaaagtgt aaaaaggtgc agaaacatac
    22201 atgtatcctt tttagaccaa ggaattttat ttgattattt aagtaatgga ggttttgaaa
    22261 aatacattaa aaaaatacgt aaattctatg gagacaaatt taactttgca tataaatgta
    22321 taaaaaaata tataaaaaca gaatatatct tgggtgatgg tggtttgcat atatttataa
    22381 aactaaaaaa tatagatact agagttttat tagaaaaatg ttatgaaaaa aatgttatat
    22441 ttatgcctgg agacttattt tttacagata actctggttt tgatacttta aggctcggat
    22501 tttctaggtt gtcttttgaa gacattgaaa ttggaataaa aataattgga gaaactataa
    22561 atgaaatgtt ctaggttaac taaatattgt tagcctagtt tatttttatg aggtataata
    22621 tatatagttt atatgaatct aatactgggg gataatatga gtaaaaagtc aaaacgaaga
    22681 aaaattaata aattaaaatt aacgattgta ataactgtat tatttcttgc tttattatct
    22741 atatatgaga tactgttctc taatcccaac aaaaaaggta atataaagag tcaagaaact
    22801 atgcaaaata aaaataatac aacagatgat tctaaaaata atcaaagctc taccaaaaag
    22861 caggaagata ataatatcaa acctactttt tcatattctt caataccaga taatataaaa
    22921 aataaaatga ttggtaaatc tatgcctact gatgaaccta taagctttga tagtctatct
    22981 tatttaaaac ttacatatta tggatttgat gaaaaaactc atcaaggaga aatgatagta
    23041 aacagtgaac tagctccaga agtagtggat atttttaagg aattatacga aaaaaaatat
    23101 ccaattgaaa aaattaagtt aatagatgac tatgatgctg tagatgaaaa atctatgtct
    23161 gataataata cctcatcatt ttgttataga actatagctg gtacaaatgt agtttcaaat
    23221 catggtaaag gacgtgctat agatataaac ccattacaga atcctcaagt ttctggaaat
    23281 gatgttacac ctaaagtaag tacagtttat gctgataggt ctagcacaaa atttggaatg
    23341 ataaaaaaag gtgatgattg ttataatgct tttgtaagca gaggttggag ttggggtggc
    23401 tattggaaaa acccagatta tcaacatttt gaaaaataat agattagaaa taaaggtgaa
    23461 ttatatgaat ttaatgacat tggaaaatat aagtaaaagc tattctgaaa aaaaattact
    23521 agaaaatata tctcttggaa taaatgaagg ggaaaaaata ggatttattg gtgtaaatgg
    23581 tactggtaaa tctaccttac tcaaaattat tgcaggagtt gaagaaagtg agactggtaa
    23641 tataacaaaa actaatggta ttaggataga atatctacct caaaatccaa cttacaatga
    23701 aaattcaact gtacttgaac aagtatttaa tggaacttct gaagaattaa agctacttgg
    23761 tgaatatcaa gaaatcctag aaaaacttaa ttcaaacttt acagaggaat taaataaaaa
    23821 attattatca gtacacgaaa aaatagatgc cttaaatctt tgggatttag aaagtgaagc
    23881 taagtcagta cttacaaagc ttggtataaa tgactttaat caaaaaatag cagaactttc
    23941 tggtgggcaa agaaagagag tctctctggc atcagccctt attacacctt gtgagttttt
    24001 gatactagat gagcctacaa accatctaga caatgatact atagattggc ttgaggaata
    24061 tcttaattcc agaaaaggtt cattgcttat gataactcat gatagatact ttttagaccg
    24121 tgttacaaat agaattattg aactggacaa aggtagatta ttcagttatg atgggaatta
    24181 ttcaatattt ttggaaaaga aaatggaaag actttcgcta gaagcaagta ttgaagataa
    24241 aagacaaaat ttaataagaa cagagcttgc gtgggtaaga cgaggagctc aggctcgtag
    24301 cactaaacaa aaagctcgtt tacaaagatt tgatgaactt ataaatagag attcttttaa
    24361 acctgatgaa aatctcgata tatctgttgc atcaagtaga cttggaaata aaataataga
    24421 aatacacaat atatctaaat catttgaaga aaatactgtt atagataatt tagaatacac
    24481 tttaactcgt acagatagaa ttggtataat aggcaaaaat ggtatgggta aatctaccct
    24541 tataaatata cttaatggag aaatagagcc tgatagtgga tatatttcaa ttggagaaac
    24601 agttaaaata ggttgttttt ctcaagacga ctctcatatg aatattgata tgagggctat
    24661 agactatgta aaagaagcta gtgactatat agaaacttca gatggtacta ggattacagc
    24721 ttctcaaatg tgtgaaagat tcctttttaa tggtactatg caatatactt tgataggtaa
    24781 attatctggt ggtgaaagaa gaagactaca tcttcttaaa atccttatga cagctcctaa
    24841 tgtgcttctt ttagatgaac ctacaaatga tttagatatt gaaacgctta aaatattaga
    24901 agaataccta gacgaattta aaggtgtagt agttacagta tcacatgata gatatttctt
    24961 agatagaatt tgtaataaaa tatttgcata tgaaggtaat ggaaaaattc atatctatac
    25021 aggtaattac agtgattatc ttatttatag agagattcaa ggaattgaat ttgaagaatc
    25081 taaaagggaa tcagttaaaa aagatgactc tactacccca aaaaaagaaa aacctaaaaa
    25141 tgataaaaag cttaagttct catataatga acaaagggag tttgaaaata ttgactctga
    25201 tatagaaaaa ctcgaatcta aaattgcaac acttgatgaa agtactgcaa aattttcaac
    25261 tgattttact aaactccaag aaattttaga tgagaaatct aaactagaaa aagaacttga
    25321 atacaaatat gaaagatggg aatatttaaa taatctagct gatgaaatag ctaataataa
    25381 gtaatattaa aataatctta agtttaataa gttttgcttt aaatctgtaa tttataaata
    25441 ttttaaagga agtgtctcaa aatgaacttt ttagttttat gagacactct tttttatgag
    25501 aaatcaaata tattttcatc atttcaacaa tacaaaagag gcttatctct attttgagac
    25561 agcctctttc ataattacct tctaatttat atgctctaat atatctataa taattcttat
    25621 tatatctata tatccagtct ttcaatatag tgacaattaa cttcaatatg atttaaaaat
    25681 actaacaacc tttcataaag tggatgagat tcttctccat atttagcttc taggttcttg
    25741 cctatttctc taacagtcct ttgaccatca atttgtaaaa aaacaaaact ggaatattca
    25801 tctagttcta tttttttgta tttaggtatt ctagctttta actttctaaa aaacttttga
    25861 attttatggt cctgtttttc aagtatagta acaacacgat tttcagctac ttcatattca
    25921 agtccatcag taattttaaa tacaatatct aaaacatcat tactcttgct cattattttt
    25981 tactttagat aaaacaatag gtaatgctgt aagcacaact aaaactacta ataaagcaat
    26041 tgccattgaa tttgttgcag caaatccact tggtaccttt ggagttacaa ctccagttac
    26101 ttgtaatatt atacctatta aaccaatgat agaaccacca gcaacaagac cagaagataa
    26161 acttatacca ttagaaactt taacttcttt ttctttttca gttttagata ctaattcaac
    26221 aaataaacga attaatgctc caactaatat tatagaagta gttgcaattg gtaaatagaa
    26281 tcctatagct atagtcatta ttggtaaatt taatgcatat agaactattg ccataaatac
    26341 tccaacaata atcataaccc aaggtaaact acctgacata ataccagaag ttaatgttga
    26401 cattaagttt gcttgtggta atgcaaattg tacattatca ccagttgctc taagttggtc
    26461 agaaagtaat aaaattactc ctacaacaac tataacacca actatacttg caattgtaaa
    26521 gtaacgttgc atttcgtttt tgctaccacc aataatataa gttacttttt gagattgagt
    26581 ataacctcca gctatagcaa tagcaacaac tataaatgaa ccaaataaaa gtaatgattt
    26641 attagcttct aaatcagtcc atcccattat aacaaacact aatgttacaa taactagaga
    26701 agctatagtc ataccagaaa ctggaagatt tgatgttccg attgttcctg ttaaacgacc
    26761 agctactatg acaaataata aagataataa aagagatatg atagctccaa taattgccat
    26821 aacaatgtta ccagaaatta agaatgctgc taaaaatcca ataactactc cacctaataa
    26881 aagtatcatt tgtatagatg aaccttcttc tccttctcca gcacttgatt tagctttaag
    26941 tgtttctttt atagaagcga ttatagttgg tataagtttt attgcaccaa taatacctcc
    27001 acaaagcatc ataccagcac ctatatattt tacataacta ctagatattg ctccaacatc
    27061 catttggtta agaggcattg caggattatt ccataccata gctccatctt tagccatatc
    27121 tgtaaagtat cctattaaag gtgctatacc aaagtttgat aaaatacttc ctgcaaacat
    27181 tgttaaagaa acttcaagcc ctactataaa tccaattcct aaaagtagag gatttacttc
    27241 aatatcaaat ttccatttgt agaatttgct tcctacaaga ctcattacat tgtttgctac
    27301 atttaagaat gaaccagtta atacagtaat aaatccactt attacaaatc caattcccat
    27361 gtacttaatt gaatctccac cagcctctga agcaacaagt gtctcagata tagccattga
    27421 ttctgggtac ataagtttac catgttcttc aactattaga tagttgtgaa ctatagcagc
    27481 aattcctata ccaaataaaa ctccaccaac tccaacagct aagccttcta agaatgtaat
    27541 ttggcttcca attaatataa ctgctggtaa tacgaaaata aatccacttg caacagattc
    27601 tccaccactt gacatacctt ggattaagtt tttaccaaga ataccctttg aacgagcaaa
    27661 tgctccaaca aaagcagaac ctattatagc tcctggaata cctgcggcaa ctgtaagacc
    27721 ggctttcatt ccagagtaag ttgtagatgc ggcaaaaatg gcagctaaaa taataccgat
    27781 aatcagtaca gctacatttc caccggttct ggacttatct gtgatatatg gaacgtaatc
    27841 ttttccacta acctcaccat aagcaccctt aggtaatttt ttgttcataa aattttcacc
    27901 ccttttcaat ttgtatatat tataacatat atacatataa aacttaactt tttattattt
    27961 tacaaatagc gagtcaaaac tactaaaatt taaccctata ttattaattt ctacttttag
    28021 atattt
//
LOCUS       DAYAPA010000044        27735 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.044, whole genome shotgun sequence.
ACCESSION   DAYAPA010000044 DAYAPA010000000
VERSION     DAYAPA010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27735
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.044"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            66..383
                     /gene="flgB"
                     /locus_tag="ACGI59_001746"
     CDS             66..383
                     /gene="flgB"
                     /locus_tag="ACGI59_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HFG9739532.1"
                     /translation="MNTYDIIKMGLDATNLRSQTIANNIANINTEGYKRHYITFEETL
                     NDAMPKIEEKTDNSKGLRVDGNNVDIEEEKVNQAMATLQYNALISFANGKIAMTKSII
                     SGR"
     gene            391..795
                     /gene="flgC"
                     /locus_tag="ACGI59_001747"
     CDS             391..795
                     /gene="flgC"
                     /locus_tag="ACGI59_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HFG9739533.1"
                     /translation="MSVFSGMRISASGLTAESLKMDIISSNVANINATRGPNGEAYRR
                     KVVSFEENYDNKLGMLGVKVTSVEDDKSPLKEVYDPSHPDAKPNGYVEYPNVNILVEM
                     TDLITASRAYDSNVDTLNAQKSMISKALEIGK"
     gene            805..1122
                     /gene="fliE"
                     /locus_tag="ACGI59_001748"
     CDS             805..1122
                     /gene="fliE"
                     /locus_tag="ACGI59_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HFG9739534.1"
                     /translation="MPITQINNLMQGAKLDNLIGEAKPLTLNNKEEKKNFEDVIIDTI
                     DKVNFQQVTADDMKEKFIKGEDVSMHEVMLIGQEAQLSMQYLIEVRNKIHDAYQEMNK
                     VQI"
     gene            1147..2697
                     /gene="fliF"
                     /locus_tag="ACGI59_001749"
     CDS             1147..2697
                     /gene="fliF"
                     /locus_tag="ACGI59_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HFG9739535.1"
                     /translation="MITSFKNKIKEQVAKSRTKISSLKKGTKIALGIGVIAICLALVF
                     TAISASKNKYAVLFSELDAQDAADISAELESKKIDTKVKGSTIYVPRTEVDKLRLELA
                     PSLTGGSKGFALMDDSSSIGLTDEEFQIKKQRMIQGEIEKTIKSFPQVEDTRVHITFG
                     ESSVFEKEAVPGSAAVYLMLKPGTTLEKNQIKSVMALISGSVTNIPEKNVQVIDNKMN
                     LLSEGILEDENVDENNQPINSGISVDKNKASEQALNKQLEKSILEMLEPIFGKGKVKA
                     TVNADLSFDTAEKTEIKIDPDKVAIKETRSKNSSKSAANNVQGVDANMNNQGNNNGTN
                     TEDSLDESFEYETGKIETHTIVAPGELNRLTASVAIDGKIAKGVQADVEDIVNNAIGM
                     DGARGDTLSVVSMVFDPEGKKEAKQELEEIKKEGFKKNIIKAVIGVVAVIAVGAIGYL
                     IYKKRSEREDMDYEDEDYISKSDSVDRKDNPLLGDDPELTLEEAVKMYAEEKPDQVTE
                     IIKTWLNE"
     gene            2710..3780
                     /gene="fliG"
                     /locus_tag="ACGI59_001750"
     CDS             2710..3780
                     /gene="fliG"
                     /locus_tag="ACGI59_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HFG9739536.1"
                     /translation="MSTEITNVNAFDLSDINESDKNEKVEGEGLKKAAVLLMTLGPNV
                     SSDIIKSLPDKQVQKIGLEIANISSIASKERREILKEFVEINRAKDYVIEGGLEYARE
                     LLSNALGNVKANKLLEGISINTSTKPFKHIIKLDVKKILEILRNESAQTIAVLLAHME
                     PENGAEVLSYLDFDLQNEVAMRISSMGSISPEVIKVIESSLEDKLHSISGDDFNDYGG
                     VSTLVQILINSDRKTEKNILGTIEEQNPDLAAQIKSHIFVFEDIVKLDDISIQKVLKE
                     VTMKDLVYALKGVDDEISDAIYRNQSSRAADALKEEMEMVGVIKVTQVEASQQKIANI
                     VRKLEREGAITIVRNFGGDFVE"
     gene            3764..4504
                     /locus_tag="ACGI59_001751"
     CDS             3764..4504
                     /locus_tag="ACGI59_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliH/SctL family protein"
                     /protein_id="HFG9739537.1"
                     /translation="MTLLNNKIIKSKKALYKGVLDLTIIDKVRVGESNVNKENDCRFR
                     DEVIKEAIESAEIRKNEILEEAKMNAQNIEKQAYEKGYSQGQKNGYEDGYKESYDEYT
                     QKAKDEADFIKNQADLILLQANEKMTEYIKEKNKEIVRLAINMASSVLKKHFEDEESM
                     SDLLKQIIQDYEGSSSFIVRCNSFYKDNIEKEFELFKEETSLKSSVFVIADDSIDKGN
                     AIIQKENGRIIVGIDCAMEKLKEELLGE"
     gene            4508..5827
                     /gene="fliI"
                     /locus_tag="ACGI59_001752"
     CDS             4508..5827
                     /gene="fliI"
                     /locus_tag="ACGI59_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HFG9739538.1"
                     /translation="MYKTDISIDFDKINKKIENLELTISEGRVKKIIGLTVEVEGIKA
                     FVGELCVIYNQVNKPVNCEVVGFKDKEVILMALGELTLIAPGCKVISKGIPLSVMCSD
                     NLLGKVLDGLGNPIDNSDAVLGDRYNLNNEPPDPMKRKKIRNIMETGVRAIDAFTTCG
                     EGQRIGIFAGSGVGKSTTLGMIARNAKADVNVIALIGERGREVLDFIDKDLGEEGMKK
                     SVVVCATSDKAPLVRLKGALTATAIAEYFRDQGKKVILMMDSVTRFAMAQREVGVAIG
                     EPPAQKGYTPSVFAMLPKLMERTGTSDKGSITAFYTVLVDGDDFNEPIADTTRGILDG
                     HIVLSRDLANKNHYPSIDVLNSLSRLMNEIASKEDIKIASFARDMLAEYREAEDLINI
                     GAYASGTNKKIDEAIYYHEHIINFLKQGINEKSSFNETISSLRRIFE"
     gene            5859..6305
                     /gene="fliJ"
                     /locus_tag="ACGI59_001753"
     CDS             5859..6305
                     /gene="fliJ"
                     /locus_tag="ACGI59_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012815984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HFG9739539.1"
                     /translation="MELDKNFKFRLQKVLDLKIKDEEEIKMEFAKIQQKKIDIETNLE
                     NLESNYSKYSISKNNDNVQNQKITINYLLALNNSIMDLSEELDKSTNELEKARKQLIS
                     KQIERKSLEKLKEKKYGQYYKEEQLKEQSTNDEFASMSYLRNRQVL"
     gene            6333..7538
                     /locus_tag="ACGI59_001754"
     CDS             6333..7538
                     /locus_tag="ACGI59_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HFG9739540.1"
                     /translation="MKIQADSYNIIKDIGNRKNSSKNKNIKNNKFEDNLYKTTDHKNV
                     KDRRETSKQNVSSDRPGNSDNECHLDEDKSNHLEDENTYEKDCSKNNEALQQLLNLLK
                     ENYKKDKDINTILNNIKKLSISDELKQELKLNKNILNKIANQNLELNGANTNNPNDIE
                     NLDIMKLRELLENNASEDIQVISKTTENDTKSIDLYSFDSNRMDTLNSNKQKDDSSDI
                     LEKLAGLNGNKSGNFGQVINKSVDMNKIKNDNVNLKQIESNVMDDSIKAIKHMKTNDI
                     QELTIKLRPKELGDMNIQLLKDGESMRAVVTVFNKDVFDSINKNIADLKQHLELTNVN
                     IKDVSVQMHSDNTNTSDTFDKAFEQQNRQNNQENMSNQNTNDKRNNVVIEDEIKENII
                     DDDRVDLLA"
     gene            7556..8164
                     /locus_tag="ACGI59_001755"
     CDS             7556..8164
                     /locus_tag="ACGI59_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HFG9739541.1"
                     /translation="MYGIATALAGTQVNYRSNNLSQLERNINDIANKQVRSKGNQTVT
                     DNGTPIIGQGDDADKDLFMKILVAQMGNQDPMNPQDPTEYVSQLAQFATLEKMSAMND
                     KLDILLALSNSTLINSALSMATSLIGKEVEVSGEEDEDENIVGVVKSTYIKDGEVHLE
                     LEVDGSDKTVDVKYEKLLKIGEVEANSGEENDSSEQESPEEV"
     gene            8191..9174
                     /locus_tag="ACGI59_001756"
     CDS             8191..9174
                     /locus_tag="ACGI59_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HFG9739542.1"
                     /translation="MIKAMYSGVSGMKANQTKLDVIGNNVANAGTTSFKKSSARITDS
                     FYQTVLYASAPTAALGGTNLGQVGVGSKISSINKDMTQGNVQPTNRPSDLMIDGDGYL
                     PVVRQGTVMYTRDGSFNLDTGFKAADGALKNIKGGRLVNGDGYMLQGMIYDGEYKADT
                     GEFVKKGDAKGKEPIVIPLQSISPKDGTVQNVLEYNISKDGTVEFLLSDGQRTQFVYD
                     IPDGAKVGDTPAGIDKLKGTIQKVQTYAFQNPAGLDAEGGNLFKPSANSGDPQIAGAT
                     GTIIQGAIEASNVDIAEEFTEMIIASRSFQANSKIISTSDEILQEIINLKR"
     gene            9187..9390
                     /locus_tag="ACGI59_001757"
     CDS             9187..9390
                     /locus_tag="ACGI59_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FlbD family protein"
                     /protein_id="HFG9739543.1"
                     /translation="MIRVTDLNGRKYILNSDLIVRIDSVPESRILLTTGEKNLVKESV
                     DEIVDKVIKYKRKINVGYIRSEK"
     gene            9390..10208
                     /locus_tag="ACGI59_001758"
     CDS             9390..10208
                     /locus_tag="ACGI59_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HFG9739544.1"
                     /translation="MKKTDSLTFIGLILSTVLVLFGAVKGSSSGLKSFFDFASILITV
                     FGSFGALMITFTIDDIKLIKDALQYSFKTMNISKLDLLNQFKTLSKKARREGLLSIES
                     DVMKIQDPFMKKGLELCIDGLEINEIRSILELEIDSVEETNNRYSKLFKTWGTFAPAF
                     GMLGTLIGLIQMLSDLTSPDLIASGMGKALITTFYGSLLANIALNPIAYNIDEKTEKE
                     IYVKEMMLEGIISIQSGESSIVVEERLATYLSNNEKLEIMKSNKNTERAMSNGA"
     gene            10198..10893
                     /locus_tag="ACGI59_001759"
     CDS             10198..10893
                     /locus_tag="ACGI59_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFG9739545.1"
                     /translation="MARKKNKKKDEINPDAWLATYADTITLILTFFVLLYATSVVDME
                     KFKNIADALKGQFSGTSMFSTGSSTSEKSPIESVLVDKNDKSNLEQDLKDKVNNSNLQ
                     NSVTVKDDSRGILLELDDSILFDSGISELKEDSKATLNKVYDMIKMMKNKIVIEGHTD
                     NVPVKSSTHESNWDLSSSRAVSVVRYFVEEKGMDPKLFSATGCGEYQPLVSNETAKGR
                     AENRRVNILITID"
     gene            10905..11348
                     /gene="fliL"
                     /locus_tag="ACGI59_001760"
     CDS             10905..11348
                     /gene="fliL"
                     /locus_tag="ACGI59_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HFG9739546.1"
                     /translation="MFKNKKIIIIILILIIFSAGAGFFILGEGGNKQIDKIAGLFIPT
                     GKGKDEVKTENLDLEEMVLKLADEGVRYLKVSITVSYDSSYKDIEKNTPLIRDNIINC
                     FMAKRADDFTADNLKNIKEEIKNTLNIQLGENVIQNIYFTNIVVQ"
     gene            11363..11737
                     /locus_tag="ACGI59_001761"
     CDS             11363..11737
                     /locus_tag="ACGI59_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliZ"
                     /protein_id="HFG9739547.1"
                     /translation="MILYLLKILICIPIILILIVLSLKISQGTLLKKYNDKYIKILDI
                     VNISKNNSIIILKIGEEGCIMASSSSSMSKIKDLTKEEILRIENDIKERNFKIQDFKM
                     ERIKKKYDFSRYIGKFTKKEDK"
     gene            11738..12403
                     /gene="fliP"
                     /locus_tag="ACGI59_001762"
     CDS             11738..12403
                     /gene="fliP"
                     /locus_tag="ACGI59_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425215.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HFG9739548.1"
                     /translation="MELLSKLPLADVPYTSSMQLFLFLTALMFLPFVMFMMTSFVRIV
                     ISLSFLKSALGAQQAIPSQILVGLAIVLTIFIMRPVLNEINEKALQPYMKEEVTMEEA
                     MKEAEGPIKEFLLKQTRQTDLDLFVEQAGLKEKKLTRENIPLSVVVPAFAISELKTAF
                     QIGFLIYIPFLIIDLVVASVLMSMGMFMLPPVMISLPFKLLLFVMVDGWNLIVKTLIL
                     GFG"
     gene            12416..12685
                     /gene="fliQ"
                     /locus_tag="ACGI59_001763"
     CDS             12416..12685
                     /gene="fliQ"
                     /locus_tag="ACGI59_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HFG9739549.1"
                     /translation="MSEFMIMSVLKNGMFTGAMIAAPVLIVSLVVGLLISVFQATTQI
                     QEQTLTFVPKLIAILLVVAIGGSWMLHTLISFTSEIFNMMSLITK"
     gene            12697..14511
                     /locus_tag="ACGI59_001764"
     CDS             12697..14511
                     /locus_tag="ACGI59_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused FliR family export protein/FlhB family
                     type III secretion system protein"
                     /protein_id="HFG9739550.1"
                     /translation="MIFVFIRIVAFFGTLRILFPAGTPALFKSLFAIIISILISSTMQ
                     IEYATNIDNIVLFTLYGINETITGIMLGYITNLCFYSIRMAGSMMDQQMGLSMINMFD
                     PNSMTQTTLINNLMNWTALMIFFSMDGHHVLIRGIRYSFELIPIGKSFIDNNIDYIIN
                     IFVQCFLTGFKIAIPIVLCLLMADFILGLISRSVPQLNVMIIGMPLKILVGIALFIIS
                     IPLIVNQISHLLSQIPKMYEGTFALAPMFFMGSTDKTEEATPKKKGEQRKKGNIAKSK
                     ELPIAMTLLAFTLLVPTLFSYVVNTLKSSLNYFLSLDFYMNINYSSIEKLVIAGLMDF
                     FKIFLPIAIPFLVLGIVANLFQVGILFTGETLKPNLSKLNPISGFKNMFSMRSLSTLI
                     KDTAIISILAYIGYTFFQDNYLDILKLGNIYLPTLMYTVKDLVYSILSKICVAMIVIA
                     VADYVYQRYSHKKQLRMTKQEVKDEYKNSEGDPEIKAKIKQKQRQISSQRTMQAVPSA
                     TVIVTNPTHLSIAVRYEKGKDQAPVVVAKGADYLAFKIREIAKGNDIPIIENKPIARL
                     LYKQVEIDQEIPEDMYQAFAEILVAVYKIKNRYKVPKR"
     gene            14520..16595
                     /gene="flhA"
                     /locus_tag="ACGI59_001765"
     CDS             14520..16595
                     /gene="flhA"
                     /locus_tag="ACGI59_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HFG9739551.1"
                     /translation="MNKFNLKENMDVIVAFGVVGIVLMMIVPLPTFILDILLAINISL
                     SVLILLMTLFTTNVLDLSIFPTVLLVTTLFRLGLNLSSTRLILTQGYAGEVIEAFGSF
                     VVGGNYVVGIIIFLIILIVQFVVITNGSGRVSEVTARFTLDAMPGKQMSIDADLNAGV
                     IDDKTARKRRKELQQEADFYGSMDGANKFVKGDAIAGLIVTAINIIGGIVIGAVMLNM
                     TLMGAAQTYVRLTIGDGLVSQIPALLISTSAGIIVTRASTDENFGGLVGKQLTAIPKA
                     VMMTGCVLIVLGMMPGLPKPAFFSLGIGAIAAGYFLGREKKDTLEDLEFEKPDMVNPD
                     KNEVEDVTNLINVESIEVEIGYGLISLADESAGGDLLQRIASIRRQCAIDMGIVVQPI
                     RIRDNLQLNPNQYNIKIKGNVVGGYEIMPNMVLCMDPMNQGFSIFGIKTIEPTFGIEA
                     LWIESDKVEDAELKGYTVVDSSTILITHLLDIIKSNAHELLGRQEVKTIIDAARENYS
                     AVVDELIPDLMTLGEIQKVLKNLLREKVNIKDRVTILETLADQSRNTKDIELLTEYVR
                     IALARSICNNLVDEDKAIVVATLSPETEELVSNNLQRSVNGTYPAVDPENTNKIFESI
                     QEVMNNVYFNHNIPVIVVSPKIRAPFRKLVEIVYPNLTVLSLNEIPNDIKIKAEGVVS
                     IYDDEKVYS"
     gene            16570..17586
                     /gene="flhF"
                     /locus_tag="ACGI59_001766"
     CDS             16570..17586
                     /gene="flhF"
                     /locus_tag="ACGI59_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HFG9739552.1"
                     /translation="MMTKKYTANTIQDAMNLAKMELGDNITLIDKKEVRKSGIRGIFS
                     KKDIELTIGWEKKDNLEQKDLKREIEQLKSIINNMGFDNKNDNDIDKICKNLLNLDLN
                     QEIVEFIKADLQEMKFNGIDTSKNLVESLKKKIKIENQAINGKIALVGPPGVGKTTTI
                     AKLAAKLVFEENKKVGVITIDTYRIGAVEQLKIYTDIMNIPFKGVISPDEMELALDEM
                     KDCDVVLIDTTGRGYKNSMQILEIKNLIDKAETDNIHLVLNCTTRESDTKAIIDSYRN
                     VNFKSLIITKLDETITYGSIFNIMNYAQKPISYITTGQNVPDDIIKPNEDKIIRLLLG
                     VESI"
     gene            17583..18461
                     /locus_tag="ACGI59_001767"
     CDS             17583..18461
                     /locus_tag="ACGI59_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HFG9739553.1"
                     /translation="MIDQAQILRKMAMEKRGSDEIIVENENMPKIITIASGKGGVGKS
                     NLATNLSICLTKLDKKVLILDADIGMSNIDIIMGVNVKGTIIDVINGEKNIEDIISQT
                     KYGVNIISGGSALNHIEDFTEAQRNKFIHSIEQIHNVDFIIIDTGAGMSKSLLSFIYC
                     STEFFLITTPEPTSLTDAYSLLKAISNFGIKKSANIIINRVIDLEEAKSTYKRIKMVV
                     DKFLNLNLELYGYIIDDKKVGVCVKKQIPFILEYPTSIASKCIIAIAKRMVSQSREDI
                     VDNKSFLRKMFSIFKS"
     gene            18475..19173
                     /locus_tag="ACGI59_001768"
     CDS             18475..19173
                     /locus_tag="ACGI59_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFG9739554.1"
                     /translation="MNREELIKENMPLVKSIARKFFIPGKGFEYQDLVNSGVIGLIDA
                     INKFDTEKGAKFSSYSYIKIKSAILDEIRNQSPISKHNLTKVNKYNRVVEKLQSKLLR
                     EPTSYEIAKELKVSEKELHDIESNIDMLNIVSLNYVIFEDTNETVQDVISDREEEAPE
                     NIIEEEKLEILSKAISNLNEREKLILSLYYYEDLNLKEIGKVLGVSESRVSQLHRKSI
                     RNLRNKIKELKYSI"
     gene            19189..19611
                     /locus_tag="ACGI59_001769"
     CDS             19189..19611
                     /locus_tag="ACGI59_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein"
                     /protein_id="HFG9739555.1"
                     /translation="MIQIILIVVSILIIVTIVINLQKESKNKDNRYFDKIFEQEYEAN
                     DRLIIAEKRRFFLDKVLAEKRINSHIKVYENYNLIKANEERKENIFIESKKEVRQENE
                     LSLKVKHLISSGYDDVEICKMLDIGKGELLLIRSCYKI"
     gene            19596..19781
                     /locus_tag="ACGI59_001770"
     CDS             19596..19781
                     /locus_tag="ACGI59_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein"
                     /protein_id="HFG9739556.1"
                     /translation="MLQNIRILLCDKKVFLGIGIGILISTLCIMLFSNNDMSKAEIEK
                     KARALGMKYTSEMKVLE"
     gene            19822..20595
                     /locus_tag="ACGI59_001771"
     CDS             19822..20595
                     /locus_tag="ACGI59_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HFG9739557.1"
                     /translation="MIRGLYTAVSAMITNQQKQNVVVNNLANMDTNGYKSKQLLTKSF
                     DELKLEGYNNYANGTKQKQIIGDISPGISMDETITNFNQGPIKTTDNKMDVAIQGKGF
                     FLVSDAAGNQFYTRNGNFRTDNQGDLITSEGYYVLGTNTATGAVGHINVGNQKFEVAK
                     DNTISLDGNAMYKFNIVDFNNYNNLKKEGDNLYSGGGAMKVNNALTIQSAIESSNVDM
                     VSEINNMMTISREYEANQKIIQAMDSKLAKIASEIGSVR"
     gene            20616..21377
                     /locus_tag="ACGI59_001772"
     CDS             20616..21377
                     /locus_tag="ACGI59_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HFG9739558.1"
                     /translation="MYTMFYTSKTALSANQSKLDIISNNIANSDSTGYKKINMEYSDL
                     VDEVLNRPSYPTNGKDISTGTGAKATAPMRNYEQGALAPTDSKSNLAIDGEGFFRVIR
                     KDGTYAYTRNGGFNVDALGKIVDDNGNILDVQFDAGYNYNNTNIDSTNLTVSRNGELF
                     VDNKKIGKINLYQPIGTQNFISEGDSLFVATDAAQIKQVEKVDIVQGYRERSNVSLQE
                     EFVDLIATQRAFQMNSKGIKTADEMWQIANNLRAK"
     gene            21400..22281
                     /locus_tag="ACGI59_001773"
     CDS             21400..22281
                     /locus_tag="ACGI59_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HFG9739559.1"
                     /translation="MNDMKEYDFGKPQSYSRENLTCLNFLLAEFCKKYKNYIIYELKC
                     NSSMVVDSIDQVNYQTFLDRVTSSCVVVQNAMEPVMKNFSFRIDRSVADMWIDITSGG
                     TGVVKDNDRELTELDKKVLLHLMDDLVRNMHLFEGFENIQVLDTHTHINLPQLCSPTA
                     PVCVVDLKVLNDNECIGSVSLCFPYNGLEALLESISVIEFFDNDVGDDSEEFTQKIYN
                     SVSNIELSVIAEIGKVSINVEDLLKLEVGDVIVTNKKINDYIDIFVENSKSYTATPGF
                     ISSKKGVKINDAVGKEV"
     gene            22284..22658
                     /locus_tag="ACGI59_001774"
     CDS             22284..22658
                     /locus_tag="ACGI59_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HFG9739560.1"
                     /translation="MDNSNLISDEEAKMLLNDKVESEVDGDDELALDVSEVNRNNIQR
                     ILDLKLELSVVIGRTKMTLRDILNLHKGSLIELDTLADQDVEILIDNKVLAYGKVVVV
                     DLNFGVKITSIVSEEDMVKSLV"
     gene            22690..24093
                     /locus_tag="ACGI59_001775"
     CDS             22690..24093
                     /locus_tag="ACGI59_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFG9739561.1"
                     /translation="MVLILNLNDIKKDILKLNKKDSCSLYNESINNINNNCISTGLKF
                     LKQARDLNPDDVDILNLTGLVNLLKCNFDDAVESFYKSSLCEKTALCEKYINMLTSEE
                     FLIFFEKYNQLIRLINKGMYKEAIQGFKLITDQYCDLIEPYELLALLYDKEEEYIKFD
                     ECLEVLKIIDKENYLLNQENNTLDMYNSANIQTKSNIKVDDYKNEFLHKSSSNHKIND
                     YNDVTKEKSSSKNQKSSDSNKKKSIKKNKYLYIAIILGILLVGQSIYNSYKMNNLTDK
                     ISKNNETKNEESIDSNLTKKENNIEENEDMSKASNINKSNKNINKEDDVKSKKNTVEN
                     EDDKDSDLFTEDELMSKANTLKSEKKTKSSIQLYKKVADIGENKGNTSEATYQVAILS
                     EKLKDYKTAEEYYKMYVENYSEKDAYFDESYYNLGMMYYNNGDLKNSKLTLKKLVNKV
                     PNSMYNNSKVKEILKEE"
     gene            24209..26146
                     /gene="htpG"
                     /locus_tag="ACGI59_001776"
     CDS             24209..26146
                     /gene="htpG"
                     /locus_tag="ACGI59_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HFG9739562.1"
                     /translation="MEFEKGSISIHTENIFPIIKKWLYSDKDIFIRELISNGCDAVSK
                     HKRLVSLGEISENKSSDYKITVSVNKGEGTLKFIDNGIGMTEEEIKKYINQVAFSGAE
                     DFFNKYKDKMEESNDIIGHFGLGFYSAFMVSKKVQIDTLSYTEGATPVRWISEGGTEY
                     EISESDARNDRGTTITLFIDDDSKEFLDEFTVRGIINKYCSFLPVEIYLEDVERLERE
                     AKEAEEKAKKQKEDGKEEIVDAKVIEPLNDTNPLWLKSPKDCTDEEYKEFYRKVFNVF
                     DEPLFWIHLNVDYPFNLKGILYFPKLKNEFELTEGKVKLYNNQVFVADNIKEVIPEFL
                     LLLKGVIDCPDLPLNVSRSFLQNDRDVSKISKHIIKKVADKLKSLCKNEREEYNKFWD
                     DIQIFIKYGCLKDESFYEKVKECILFKTIDDEYITLQDYLEKCKDKHENKVFYVSDKE
                     QQSQYIKLFKEYDLSAVVLNSSIDTHFISFMEYKENGVKFNRIDADLSDVLKDKNENK
                     DSEENKEEIAKIEGLFKEAVGERVKNYSVEGLKNEDTPAMVLVSEQSIRMAEMQSRFA
                     GMDLGMNFEEEKTLVINENSPIIKKLVSLKDDEEKKDKITLICNQIADLALLSNKELK
                     PDELDSFVQRSNKLMSLLIEL"
     gene            26534..27664
                     /locus_tag="ACGI59_001777"
     CDS             26534..27664
                     /locus_tag="ACGI59_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HFG9739563.1"
                     /translation="MNFNYNLPVNILFGSGRINDVGKEVSKYGKKVLIVTGKNSTKKT
                     GLLDKTIDLLKDSKIEYEVFDRVEQNPLITTIYSGVEIIKSTGCDCVLGLGGGSIMDA
                     AKSIAFSYKNPGDLNDYIFGIRQGEEALPIILVPTTSGTGSEGNCFAVLTNSETKDKK
                     SIKKNSMYAKASIIDPELMVTMPKHIIASVGFDALAHNMEAYLSNGRNPLADVQAIYG
                     IELISENLIKVYNDVNDLEAWEKITLASTIGGMTIGTAGTALPHGMEHPVSGLRDVMH
                     GKGLAALTPTIIESSWDSDIERFGNISKVLGGTCAEDCSDAIRNFLKKIDLDIKLSDL
                     GIEKSDIDWLTDNCMKISVANIKRHPKYFNKEQIKEMYHKSL"
BASE COUNT        11485 a         2929 c         5156 g         8165 t
ORIGIN      
        1 atagcaactt catttttttt attaataaac aaaatagata aaatatattt tgaggagggg
       61 tgagtgtgaa tacatatgac attataaaaa tgggattaga tgcaactaat ctaagaagtc
      121 agacaatagc aaataatata gcaaatataa atactgaagg atataaaaga cattatataa
      181 cttttgaaga aacactaaat gatgctatgc ctaaaataga agaaaaaaca gacaattcta
      241 aaggtttaag agtagatggt aataatgtag acatagaaga agaaaaagta aatcaagcaa
      301 tggcaacatt acaatataat gctttgataa gttttgcaaa tggaaaaata gcaatgacca
      361 aatcaataat aagtgggagg taattagaag atgagtgtgt tcagtggaat gagaatatct
      421 gctagtggat taacagcaga aagtttgaaa atggatatca tatcatccaa tgtagctaat
      481 ataaatgcta caagaggtcc aaatggagaa gcatatagga gaaaagttgt ttcatttgaa
      541 gaaaactatg ataataaatt aggtatgcta ggagtaaaag ttacatctgt tgaagatgat
      601 aaatcacctc ttaaagaagt atatgaccca tctcatccag atgcaaagcc aaatggatat
      661 gtagaatatc caaatgtaaa tatattggta gaaatgacag atttaataac agcttcacga
      721 gcatatgatt ctaacgtaga tacattgaat gctcagaagt ctatgatatc aaaagctttg
      781 gaaataggaa agtagggata aattatgcca attacacaaa ttaacaattt aatgcaaggt
      841 gcaaagttag ataatttgat tggagaagca aagccattaa ctcttaataa taaagaagaa
      901 aaaaagaatt ttgaagatgt aataatagat actatagata aagttaattt tcaacaagta
      961 acagcagatg atatgaaaga aaaatttata aaaggcgaag atgtttctat gcatgaagtt
     1021 atgttaatag gtcaagaagc acaactatct atgcaatatt taatagaagt tagaaataag
     1081 attcatgatg catatcaaga aatgaataag gtgcaaattt agccttttga acaggagtaa
     1141 taatcaatga taaccagttt taaaaataag attaaggagc aagttgctaa atctaggaca
     1201 aagataagtt ctttaaaaaa gggaactaaa atagcattag gtataggggt tattgctata
     1261 tgtttagcgc tagtatttac agcaatatct gcgagcaaaa ataagtatgc agtcttattt
     1321 tcagaactag atgctcaaga tgctgcagat atatcagcag agctagagtc taaaaagata
     1381 gatactaagg taaaaggaag tacgatttat gtacctagaa cagaagttga taagttaagg
     1441 ctggagttag cgccaagtct tacaggtggt tctaaaggat ttgcactaat ggatgatagt
     1501 tcatcaatag ggcttactga tgaagaattt cagattaaga aacaaagaat gatacaaggt
     1561 gaaatagaaa aaacaataaa gagttttcca caagtggaag acactagagt acatataaca
     1621 tttggagagt catcagtatt tgagaaggag gctgttccag ggagtgcagc agtttatttg
     1681 atgctaaaac cagggacgac tttagaaaaa aaccaaataa agtctgttat ggcacttata
     1741 tcaggaagcg ttacaaatat accagagaaa aatgttcagg ttattgataa caaaatgaat
     1801 ttattatctg aaggtatttt ggaggatgaa aatgttgatg aaaataatca accaataaat
     1861 agtggcatta gtgttgataa aaataaggca tctgaacaag cattgaataa acaactagaa
     1921 aagtctattt tagaaatgct agaaccgata tttggtaagg gaaaggtgaa agctacagta
     1981 aatgctgatt taagttttga tacagctgaa aaaactgaga taaaaattga ccctgataaa
     2041 gttgcaataa aggagacaag gtcgaaaaat tcatctaaat ctgctgcaaa taatgttcaa
     2101 ggtgtagatg ctaacatgaa taaccaagga aataacaatg gtactaatac agaagatagt
     2161 ttagatgaga gttttgagta tgaaacaggt aagattgaaa ctcacactat agttgcgcct
     2221 ggagaactta atagattgac agcttctgtt gcaatagatg gaaaaatagc taaaggtgtt
     2281 caagctgatg tagaggatat tgttaataat gctataggta tggatggagc gaggggagat
     2341 actcttagcg ttgtttcaat ggtatttgac ccagaaggta agaaagaggc taaacaagag
     2401 ctggaagaga ttaaaaaaga aggatttaaa aagaacataa taaaagcagt tattggagta
     2461 gtagcagtaa tagcagttgg agctattgga tacttaatat ataagaaaag aagtgaacga
     2521 gaagatatgg attatgaaga tgaagattat atttctaaat ctgattctgt tgataggaag
     2581 gataatcctc tattaggaga tgacccagaa ctgacattag aggaagctgt taaaatgtat
     2641 gcagaagaaa agccagacca agtaacagag ataataaaga cttggttaaa tgagtaaggg
     2701 ggatagaaag tgtccacgga aattacaaat gttaatgcgt ttgacttaag tgatataaat
     2761 gaaagtgata agaatgagaa ggtagaaggt gagggtttaa aaaaagctgc tgtattatta
     2821 atgacattag gcccaaatgt gtctagtgat ataataaaaa gcttgcctga taaacaagtt
     2881 caaaaaatag gtttggaaat tgcaaatatt tcttcaatag cttcaaaaga aagaagagaa
     2941 atcttaaaag agtttgtaga aataaatagg gcaaaagatt atgttataga aggtgggctt
     3001 gaatatgcaa gagagttatt aagtaatgct ttaggaaatg ttaaagcaaa caaattactt
     3061 gaaggtatat caataaatac aagtacaaag ccttttaaac atattataaa actagatgta
     3121 aagaagatat tagaaatact taggaatgaa agtgcacaaa ctatagccgt tttacttgca
     3181 catatggaac cagaaaatgg agcagaagtt ttatcatact tagattttga tttacaaaat
     3241 gaagtagcta tgagaataag ctcaatgggt tcaatatctc cagaagtaat aaaggtaatt
     3301 gaaagttcat tagaagataa attacattca ataagtggtg atgattttaa tgattatgga
     3361 ggggtgtcta ccttagttca aatattgatt aattctgata gaaaaacaga aaagaatatt
     3421 ttgggaacta ttgaagaaca aaacccggat ttagcagctc aaattaaatc tcatatattt
     3481 gtctttgaag atattgtcaa gttagatgat ataagcatac aaaaagtgtt aaaagaagtg
     3541 actatgaaag atttagtata tgccttaaaa ggtgtagatg atgagatttc agatgctata
     3601 tatagaaatc aatcttctag agcagcagat gcacttaaag aagaaatgga aatggttggt
     3661 gttataaagg ttacacaagt tgaggcttct caacaaaaga tagctaatat cgtaagaaaa
     3721 cttgaaagag aaggtgccat aactattgta aggaattttg gtggtgactt tgttgaataa
     3781 taagattatt aaaagtaaaa aagcattata taaaggagtg ctagatttaa ccataataga
     3841 taaagttaga gtgggagaat caaatgtaaa taaagaaaat gattgtagat ttagggatga
     3901 ggtaataaaa gaagctatag aatctgctga gataagaaag aatgagatat tagaagaagc
     3961 aaaaatgaat gcacaaaata tagaaaagca agcatatgaa aaagggtata gtcaaggtca
     4021 aaaaaatgga tatgaagatg gatataaaga gtcatatgat gaatataccc aaaaagctaa
     4081 agatgaagca gattttataa aaaatcaggc agacttaata ctattacaag ctaatgagaa
     4141 gatgactgag tacataaaag aaaaaaataa agagattgta agacttgcta ttaatatggc
     4201 tagttctgtt ctaaaaaagc atttcgaaga tgaagaatct atgagtgatt tgttaaagca
     4261 aattattcaa gattatgaag gaagtagttc atttatagta agatgtaatt ctttctataa
     4321 agataatata gagaaagagt ttgaattatt caaagaggaa acatcactaa aatcaagtgt
     4381 atttgttatt gcagatgata gtatagataa aggcaatgct attatccaaa aggaaaatgg
     4441 aagaataata gttggtattg attgtgctat ggaaaaacta aaagaagaat tgcttggtga
     4501 ataaagtatg tataaaactg atatatctat agattttgac aaaataaata agaaaataga
     4561 aaatttagaa ctcactatat cagaaggtag agtaaagaag ataataggac ttactgtaga
     4621 agttgaagga ataaaagctt ttgttgggga gttatgcgtt atatacaacc aagttaataa
     4681 accagtgaat tgtgaagtag taggttttaa agataaagaa gtcatattaa tggcgttggg
     4741 agaattaaca ttgatagcac ctggttgtaa agttatttct aaggggatac ctttaagtgt
     4801 tatgtgtagt gataatttac taggtaaagt acttgatggt cttggaaatc ctatagacaa
     4861 ctcagatgct gttttaggag atagatacaa cctgaacaat gaacctcctg acccaatgaa
     4921 gagaaaaaag atacgaaata taatggaaac aggtgtaaga gcaatagatg catttactac
     4981 ttgtggtgaa ggacagagaa tcggcatatt tgcaggaagt ggagtaggta agagtacgac
     5041 tctaggtatg attgctagga atgcaaaagc agatgtaaat gtaattgctc ttattggaga
     5101 aagaggaaga gaagtactag attttattga taaagattta ggtgaagagg gaatgaaaaa
     5161 atctgttgtt gtgtgtgcaa catcagataa agcccctcta gttaggttaa aaggtgctct
     5221 tactgctact gcaatagcag aatattttag agaccaaggc aaaaaagtaa tacttatgat
     5281 ggattcagta actagatttg ctatggcaca aagagaagtt ggtgtagcaa taggagagcc
     5341 accagctcaa aaaggatata caccatctgt atttgcaatg ttgccaaaac ttatggagag
     5401 aacagggaca tcagataaag gttctataac tgcattttat acagtattag ttgatggaga
     5461 tgactttaat gagccaatag cagatacaac aagagggata cttgatggtc atatagtttt
     5521 atctagagac ttggcaaata agaatcacta tccttctata gatgtattaa acagtttaag
     5581 taggcttatg aatgaaattg cttcaaaaga agacataaaa atagcgtctt ttgcaagaga
     5641 catgcttgct gagtatagag aagcagaaga tttgataaat ataggtgcat atgcttcagg
     5701 tacaaacaaa aaaatagatg aagcaatcta ttatcatgag catattatta attttttaaa
     5761 acaaggaata aatgaaaaga gttcttttaa tgaaactata agtagtttaa gaagaatctt
     5821 tgaataaaca atttaaaaaa atatttgaat aggttgtgat ggaattggat aaaaatttta
     5881 agtttagatt acagaaagtt ttagacttaa aaataaaaga tgaagaagag ataaaaatgg
     5941 aatttgctaa gattcaacaa aaaaaaatag atatagaaac taatttagaa aacttagaaa
     6001 gtaattacag caaatattct ataagtaaaa ataatgataa tgttcaaaat caaaaaataa
     6061 ctattaacta tttgctagct ttaaacaact ctataatgga tttatcagaa gaactggata
     6121 agtcgacaaa tgaacttgaa aaagctagaa aacagcttat aagcaaacag atagagagaa
     6181 agtcattaga aaaattaaaa gaaaaaaaat atggacagta ttataaagaa gaacagttaa
     6241 aagaacaaag cactaatgat gagtttgcaa gcatgagtta tttgagaaat agacaagttc
     6301 tataaaataa taggaagtga ggatattatt gtatgaagat acaagcagat tcttataata
     6361 ttattaaaga tattgggaat agaaaaaact catctaagaa taaaaatata aaaaataata
     6421 aatttgaaga taatctatat aagacaacag accataaaaa tgttaaagat agaagagaaa
     6481 caagtaagca aaatgttagt tcagatagac cgggaaatag tgataatgag tgtcatttag
     6541 atgaagataa aagtaatcac ttagaagatg aaaatacata tgaaaaagat tgtagtaaga
     6601 ataatgaagc tttacagcaa ttactgaatc ttttaaaaga gaattataaa aaagataaag
     6661 atataaacac tatattgaat aatataaaaa aactaagtat atcagatgaa ttaaaacaag
     6721 aacttaagct taataaaaat atattgaata aaattgcaaa tcaaaattta gagttaaatg
     6781 gagctaatac aaataaccct aatgatatag aaaatttaga cataatgaag ttaagagaac
     6841 ttttagaaaa taatgctagt gaagatattc aagtaattag taaaactaca gaaaatgata
     6901 ctaaaagcat agacttatac agttttgata gtaatagaat ggatactctt aactcaaata
     6961 aacagaaaga tgatagttct gatattttgg aaaaactagc agggttaaat ggaaataaga
     7021 gtggaaattt tggacaagta ataaataaaa gcgttgatat gaataaaatt aaaaatgata
     7081 atgttaattt aaaacagata gaatctaatg taatggatga ttctataaaa gctattaagc
     7141 atatgaaaac aaatgatata caagaattga caattaaatt aagaccaaaa gaattaggag
     7201 atatgaacat acaattatta aaagatggtg aaagtatgag agctgttgtg actgttttca
     7261 ataaagatgt ctttgactct ataaataaaa acatagcaga tttaaagcaa catcttgaac
     7321 ttacaaatgt aaatataaaa gatgtatcag tgcaaatgca tagcgataat acaaatacat
     7381 ctgatacctt tgataaagca tttgaacaac agaatagaca aaataaccaa gaaaacatga
     7441 gtaatcaaaa tacaaatgat aagcgtaaca atgtagtcat agaagatgag attaaagaaa
     7501 atattattga tgatgataga gtagatttac tagcttagta agggggaagt attgcatgta
     7561 cggaatagca acggctttgg caggaactca ggtaaactat aggtcaaata acttaagcca
     7621 acttgaaaga aatattaatg atattgccaa caaacaagtt agaagtaaag gaaatcagac
     7681 agtaacagac aatgggactc caattatagg acaaggtgat gatgctgata aagacttgtt
     7741 tatgaagatt cttgtagcac agatgggtaa tcaagaccca atgaatccac aagacccaac
     7801 ggagtatgta tcacaattag cacagtttgc aactttagaa aaaatgtcag ctatgaatga
     7861 taagctagat atacttttag ccttaagtaa ctctactcta ataaactctg ctttgagtat
     7921 ggcaacatca ttaattggta aagaagtaga agtatctggg gaagaagatg aagatgaaaa
     7981 cattgtagga gtagtaaagt ccacatacat aaaagatggg gaagtacact tagaattaga
     8041 agtagatgga agtgacaaaa ctgtagatgt taaatatgaa aaattactta aaataggaga
     8101 ggtagaggca aattcaggag aagaaaatga ctcttcagaa caagaatctc cagaagaagt
     8161 ataatattaa atgggaaaag aggtaaattt atgataaaag caatgtattc aggtgtaagt
     8221 ggaatgaaag caaatcaaac aaaactagat gtaatcggta acaatgtagc caacgctgga
     8281 actacttctt ttaagaaaag ttcagctaga ataacagatt cattttatca aactgtacta
     8341 tatgcaagtg ctccaacagc ggcattaggg ggaacaaact taggacaagt aggtgtaggt
     8401 agtaagatat caagtataaa caaagatatg acacaaggaa atgtacaacc aactaataga
     8461 ccatctgatt taatgataga tggagatgga tatcttccag tagtaagaca aggaacagtt
     8521 atgtatacga gagatggttc ttttaattta gatacaggat ttaaagcagc tgatggtgct
     8581 ttgaagaata ttaaaggtgg aagacttgta aatggtgatg gatatatgtt acaaggtatg
     8641 atttatgatg gggaatataa agctgatact ggagaatttg ttaaaaaggg tgatgctaaa
     8701 ggaaaagagc ctattgtaat accacttcaa agtataagtc ctaaggatgg aacagttcaa
     8761 aatgtactag agtataatat atcaaaggat ggaacagtag aatttttatt gagtgatggt
     8821 caaagaactc aatttgtcta tgatatacct gatggtgcta aggtaggaga tacacctgct
     8881 ggaatagata aattaaaagg tacaatacaa aaagtgcaaa catatgcatt ccaaaatcca
     8941 gcaggactag atgcagaagg aggaaacttg ttcaagccat cagcaaactc aggagaccca
     9001 caaatagcag gagctacagg cacaataata caaggagcta tagaagcttc caatgtagat
     9061 atagcagaag aatttacaga aatgataata gcaagtaggt ctttccaagc aaacagtaaa
     9121 ataatatcga catcagacga aatacttcaa gaaataataa atcttaagag atagtaggta
     9181 catattatga taagagtaac ggatttaaac ggaagaaaat acatattaaa ttcagatctt
     9241 atagtgagaa ttgactccgt gccagaaagc aggattctcc ttacaacagg agaaaaaaat
     9301 cttgtaaaag agtcagtaga tgagatagta gataaagtaa taaagtataa aagaaaaata
     9361 aatgtaggtt atattaggag tgaaaaataa tgaaaaaaac agattcacta acattcattg
     9421 gactaatatt atctacagta ttagttttat ttggagcagt aaaaggaagt agttcaggat
     9481 tgaaaagttt tttcgatttt gcatctatat taatcacagt atttgggtca tttggagctt
     9541 tgatgattac atttactatt gacgatataa aacttataaa agatgctcta caatactcat
     9601 tcaagacaat gaatatatcg aaattagact tattaaatca atttaaaaca ctttctaaaa
     9661 aagcaagaag agaaggactt ttatctatag aaagtgatgt aatgaaaata caagacccat
     9721 ttatgaaaaa aggattagag ttatgtattg atggtcttga aataaatgaa ataaggtcta
     9781 tactagagct cgaaatagat tctgttgaag aaaccaataa cagatattct aaactattta
     9841 aaacttgggg gactttcgca cctgcatttg gtatgttggg aactctaata ggtttaatac
     9901 aaatgttatc cgaccttaca tctccagatt taatagcatc tggtatggga aaagcattaa
     9961 ttacgacatt ttatgggtct ttacttgcca atatcgcttt gaacccaatt gcatataata
    10021 tagatgagaa gactgaaaaa gaaatttatg taaaggaaat gatgctggag gggataataa
    10081 gcatacaatc tggagagagc agcatagtag ttgaggaacg tttagctaca tacctttcaa
    10141 ataatgagaa gttagaaatt atgaaatcca ataaaaatac agaaagagca atgagtaatg
    10201 gcgcgtaaaa aaaataagaa aaaggatgag ataaatccag atgcatggtt ggctacttat
    10261 gcagatacga taacactgat tcttacattt tttgtgttat tgtatgctac atcagttgta
    10321 gatatggaaa aatttaaaaa tatagcagat gcgttaaaag gtcagttttc tgggacaagt
    10381 atgtttagta caggttcaag tacaagtgag aaatctccaa tagagagtgt tttagtggat
    10441 aaaaatgata agtcaaattt agaacaggat ttaaaagata aagttaataa ttctaactta
    10501 caaaatagtg taacagtcaa agatgatagt agagggattt tactagagct agatgattca
    10561 attttatttg actctgggat ttctgaatta aaagaagata gtaaagccac tcttaataaa
    10621 gtatacgata tgataaagat gatgaaaaat aagatagtta tagaaggcca tactgataat
    10681 gtacctgtaa aatcaagtac gcatgaatcc aattgggatt tatcttctag tagagcagta
    10741 agtgttgtaa gatactttgt agaagaaaag ggcatggacc ctaaactatt ttcggcaaca
    10801 ggatgtggag agtaccaacc tttggtatca aatgaaacag caaagggaag agctgagaat
    10861 agaagagtta atatacttat aactattgac taggaggaag aggtttgttc aaaaataaaa
    10921 aaattattat aattattttg atactgatta ttttctcagc aggagcaggt ttttttatat
    10981 taggagaagg tggaaataaa caaattgaca agattgcagg tttgtttatt ccaacaggga
    11041 aaggcaaaga tgaagtaaag accgaaaatc ttgacttaga agagatggta ttgaagctag
    11101 ctgatgaagg tgtaagatat ttaaaagtat caataactgt atcttatgat tcttcatata
    11161 aagatataga aaaaaatacg cctttaatta gggataatat tataaattgt tttatggcaa
    11221 aaagggcaga tgattttact gctgataatt taaagaacat aaaagaagaa attaaaaata
    11281 cattaaatat acaactaggt gaaaatgtaa ttcaaaatat atatttcaca aatatagtag
    11341 tgcaataaaa ggatggtaag acatgatact ttatttacta aaaatattaa tatgtatccc
    11401 aataatttta atattgatag tcttatcttt aaaaattagc caaggaactt tattaaaaaa
    11461 atataatgat aaatatataa aaatactaga tattgtaaat ataagtaaaa acaatagcat
    11521 aataatttta aaaataggtg aagaaggatg cattatggca tcttcttctt ctagcatgag
    11581 taaaataaaa gatttgacca aagaagaaat attaagaata gaaaacgaca ttaaagaaag
    11641 aaattttaaa attcaagatt ttaaaatgga aagaattaaa aagaaatatg atttcagtag
    11701 atatattgga aaatttacaa aaaaggaaga taagtaaatg gaactattat caaaattacc
    11761 tttggctgat gtaccatata caagttcaat gcaattgttt ttatttctaa ctgcattaat
    11821 gtttttacca tttgtaatgt tcatgatgac aagctttgtc agaatagtta taagtttatc
    11881 atttttgaaa tcagcattag gagcacagca agcaattcca agtcaaattt tggtagggct
    11941 tgcaatagta ttgacaatat ttataatgag accagtatta aatgagataa acgaaaaggc
    12001 attacaacca tatatgaaag aagaagttac tatggaagaa gctatgaagg aagcagaagg
    12061 acctattaaa gaatttttat taaagcaaac cagacagaca gatttagatt tatttgtaga
    12121 gcaagctggt ttaaaagaaa agaaattaac tcgtgaaaat ataccattat ctgttgtagt
    12181 tccagcattt gctataagtg aactaaaaac tgcttttcaa ataggttttt taatatatat
    12241 accatttttg ataatagacc ttgttgtggc aagtgttttg atgtctatgg gtatgtttat
    12301 gttacctcca gttatgatat cattaccatt taaattatta ttatttgtaa tggtggatgg
    12361 atggaacttg atagtaaaaa cattgatatt gggatttgga taggagaaaa atagtatgag
    12421 tgaatttatg ataatgagtg ttcttaaaaa tggcatgttt acaggagcta tgattgcagc
    12481 tccagtgcta attgtttctc ttgtagtggg gcttttaata agtgtatttc aagccacaac
    12541 tcaaatccaa gagcaaactt tgacttttgt accaaaacta atagcaattc ttttagtagt
    12601 agctattggt ggaagctgga tgttacatac tcttattagc tttacttcgg agatattcaa
    12661 tatgatgtct ctcataacta aataagtagg atatatatga tttttgtatt tattagaata
    12721 gtagcattct ttggaacatt aaggatacta tttccagctg gtacacctgc tttatttaag
    12781 tcgctctttg caatcattat atcaatcttg atttcaagta ctatgcaaat tgaatatgca
    12841 acaaatatag acaacattgt attgtttacc ttatatggta taaatgagac cataactggg
    12901 attatgcttg gatatataac caatctatgt ttttatagta tcagaatggc tggttctatg
    12961 atggaccaac agatgggatt atcaatgatt aatatgtttg acccaaattc aatgactcag
    13021 acaactttaa tcaataatct tatgaactgg acagcactta tgatattttt tagcatggat
    13081 ggtcatcatg ttttaattag aggcataagg tatagttttg agttaattcc aatagggaaa
    13141 tcattcatag ataataatat agattatata ataaacatat ttgtgcagtg ctttttaact
    13201 ggattcaaaa tagcaatacc tattgtactt tgtctgctta tggcagattt tatattaggg
    13261 cttatatcaa gaagtgtacc tcaactaaat gtaatgataa ttggtatgcc attaaaaata
    13321 ctggttggta ttgcattatt tatcatatca atacctttaa ttgtcaatca aataagtcat
    13381 ttattaagtc aaataccaaa gatgtatgag ggaacttttg cactagctcc tatgtttttt
    13441 atgggttcaa cagataaaac agaagaagct actccaaaga aaaagggaga acaaagaaaa
    13501 aaaggtaata tagcaaagag taaagaatta cccattgcaa tgactctact tgcattcact
    13561 ttattagtac ctacattatt ttcatatgta gtgaatacac ttaaatcttc tcttaattat
    13621 tttttgagtc tagattttta tatgaacata aattattcaa gtatagagaa attagttata
    13681 gcaggattaa tggatttttt taagatattt ttaccaatag ctattccatt cttagtttta
    13741 ggtatagtcg caaacctatt tcaagtagga atactgttta ctggtgaaac tttaaaaccc
    13801 aatttatcaa aattaaatcc aattagtgga ttcaaaaaca tgttttctat gagaagtttg
    13861 tcaaccttaa taaaagatac agcgattata tctattttgg cttacatagg atatacattc
    13921 ttccaagata attatttaga tatattaaag ttaggtaaca tatacctgcc aactttaatg
    13981 tacacagtta aagatttagt atattcaata ttaagtaaga tttgtgtagc tatgatagtt
    14041 atagcagttg cagattatgt ttatcaaaga tacagtcata aaaagcaact tagaatgaca
    14101 aaacaagaag ttaaagatga atataaaaac tcagaaggag acccagaaat aaaagctaag
    14161 ataaaacaaa aacaaagaca aatttcgtct caaagaacca tgcaagcagt accaagtgct
    14221 actgtaatag taaccaatcc cacccactta tctatagcag tgagatatga aaaagggaaa
    14281 gaccaagctc cagtagtggt ggcaaaaggt gcagattatt tagcttttaa aataagggaa
    14341 attgcaaagg gaaatgacat acctataata gaaaataaac ctattgctag gttgttatat
    14401 aagcaagtag aaatagacca agaaattcca gaggacatgt atcaagcttt tgcagagata
    14461 ttagtagcag tatataaaat caaaaataga tacaaagtcc ccaaaaggta ggataagtaa
    14521 tgaataagtt taatttaaag gaaaatatgg acgtaatagt ggcatttggt gttgtaggga
    14581 ttgttcttat gatgatagtg ccgttaccta catttatatt agatatttta ctggcaatta
    14641 acataagttt atctgtgcta attttactaa tgacactatt tacaacaaat gtacttgatt
    14701 tatctatatt tccaacagtg ttacttgtga caacactatt tagattggga ttaaatcttt
    14761 catcaactag attgatttta acacagggat atgcaggtga agttatagag gcatttggaa
    14821 gttttgttgt aggtggaaat tacgtagtag gaataattat attcttaatt atattaattg
    14881 ttcaatttgt tgttataacc aatggttctg gaagagtatc tgaagtaaca gctagattta
    14941 cgttagatgc catgccaggt aagcagatga gtatagatgc agatttaaat gcaggagtta
    15001 tagatgacaa aactgctaga aaaagaagaa aagagcttca gcaagaagca gatttttatg
    15061 gctctatgga tggagcaaat aagtttgtaa agggagatgc catagcaggt cttatagtaa
    15121 cagcaataaa cataataggt ggaattgtaa taggagctgt aatgctgaat atgactctta
    15181 tgggtgcagc acagacatat gtaagactta ctatagggga tggtcttgta agccagatac
    15241 ctgctctatt aatatctaca tcagcaggta taatcgtaac aagagcttct actgatgaaa
    15301 actttggtgg attagtaggt aaacagttaa cagcaatacc aaaagcagta atgatgactg
    15361 gctgtgttct aatcgtactt ggtatgatgc ctggattacc taaaccagca tttttttcac
    15421 taggtatagg tgctatagca gcaggttact ttttaggaag agaaaagaaa gatactttag
    15481 aagacttgga gtttgaaaaa cctgatatgg taaatccaga taaaaatgaa gtagaagatg
    15541 taacaaactt aataaatgta gaatcaatag aagtagaaat tggatatggt ttaatatctt
    15601 tagctgatga atcagcagga ggagatttac ttcagagaat agcatcaata agaagacaat
    15661 gtgcaataga catgggtata gtcgtacaac ctataagaat aagagataat ttacagttaa
    15721 atccaaacca atacaatata aaaataaaag ggaatgtagt tggtgggtat gagataatgc
    15781 ctaatatggt tttatgtatg gatcctatga atcaaggatt tagtatattt ggaataaaga
    15841 caattgagcc tacttttgga atagaggcac tttggataga aagtgacaag gtagaagatg
    15901 cagagctaaa gggttataca gttgtagatt catccacaat acttataact cacttactag
    15961 atataataaa atcaaatgca cacgaattac tgggaagaca agaagttaag actattatag
    16021 atgcagcaag agaaaattat agtgctgtag tagatgaatt gataccagat ttaatgacac
    16081 ttggtgaaat tcaaaaagta cttaaaaatc tgttaagaga aaaagtaaat ataaaggata
    16141 gagttacaat acttgagaca cttgcagacc agtctagaaa tacaaaagat atagagcttc
    16201 ttactgaata tgtaagaata gctttggcta gaagtatttg taataattta gttgatgaag
    16261 ataaggcaat tgtagtggct acattgtctc cagaaactga agaattggta tctaataatt
    16321 tacaaaggtc tgtaaatgga acttatccag cagttgaccc agaaaataca aataaaatat
    16381 ttgagagtat acaagaagta atgaacaatg tatatttcaa tcataatata ccagtaatag
    16441 tagtatcacc aaagataaga gctccattta gaaaattagt tgaaattgta tatcctaatt
    16501 tgactgtatt atcattaaat gaaataccta acgatattaa aatcaaagca gaaggtgtgg
    16561 tgagcatata tgatgacgaa aaagtataca gctaacacaa ttcaagatgc tatgaatcta
    16621 gccaaaatgg aattgggtga caatattaca ttaatagata aaaaagaggt aagaaaatct
    16681 ggaataagag gcattttttc aaagaaagat attgaattga ctattggatg ggaaaagaaa
    16741 gataatttag aacaaaaaga tttaaaacga gagatagaac aactaaagtc aattataaat
    16801 aatatggggt ttgataataa aaatgataat gatatagata aaatatgcaa gaatttacta
    16861 aacttagatt taaatcaaga aattgtagaa tttataaaag ctgatttaca agaaatgaag
    16921 ttcaatggaa ttgatacaag taaaaattta gtagaaagtc taaagaaaaa gattaaaata
    16981 gaaaatcaag caataaatgg taaaattgct ttagttggac caccaggagt aggtaaaacg
    17041 actacaatag caaagttggc tgccaaatta gtttttgagg aaaataaaaa agtaggagta
    17101 ataactatag atacatacag gattggtgct gtagaacagt taaagatata tactgatata
    17161 atgaatattc cttttaaagg ggtaataagc cctgatgaga tggaattggc acttgatgag
    17221 atgaaagatt gtgatgttgt attaatagat acaacaggta gaggctataa aaactctatg
    17281 caaatattag aaatcaaaaa tcttatagat aaagctgaaa ctgataatat acatttagtt
    17341 ttgaactgta caacaagaga aagtgataca aaagcaatta tagattcata tagaaatgtg
    17401 aattttaaaa gcttaataat tacaaagtta gatgaaacaa taacatatgg ctcaatattt
    17461 aacataatga attatgcaca aaaaccaata tcatatatta caacaggaca aaatgttcct
    17521 gatgatatta taaaacctaa tgaagataag attataagat tactattagg agttgaaagt
    17581 atatgataga ccaagctcaa attttgagaa aaatggcaat ggaaaaaaga ggttctgatg
    17641 agattatagt agagaatgag aatatgccta agataattac aatagcttct ggtaaaggcg
    17701 gcgttggaaa gagtaatctc gctacaaatt tgtctatatg cttaaccaaa cttgataaaa
    17761 aagttcttat attagatgca gatataggaa tgtctaatat agatataata atgggggtaa
    17821 atgtaaaagg gactataatt gatgtaatta atggcgaaaa aaatatagaa gatataattt
    17881 cacagactaa gtatggtgta aatataatat ctggaggttc tgcccttaat catatagaag
    17941 actttacaga agctcaaaga aataagttta tacatagcat agaacagatt cacaatgtgg
    18001 attttataat aattgataca ggagctggta tgagcaaaag cctattgtca tttatatact
    18061 gtagtactga atttttctta ataacaactc cagaaccgac atcattgact gacgcatata
    18121 gtttattaaa agctataagt aattttggca taaagaaaag tgcaaatata ataatcaata
    18181 gggtaattga tttagaagaa gcgaaatcta catacaaaag aataaaaatg gttgtggaca
    18241 aatttctaaa ccttaatctg gaattgtatg gatatataat agatgacaaa aaagtaggtg
    18301 tatgtgtaaa aaaacagata ccattcattt tagaatatcc tacaagtatt gcatcaaaat
    18361 gtattatagc tatagcaaag agaatggtaa gtcagtcaag agaagatatt gttgataata
    18421 aaagttttct aagaaagatg tttagtatat ttaaaagcta aggaggcgta gttaatgaat
    18481 agagaagaat taataaaaga gaatatgcct cttgtaaaga gtatagcaag aaaatttttt
    18541 ataccaggga aaggttttga atatcaagac ttagtaaata gtggagtcat tggattgatt
    18601 gatgcaataa ataaatttga tactgaaaaa ggagcaaagt tttctagtta ctcttatata
    18661 aagataaaat ctgctatatt agatgaaatt agaaatcaaa gtccaatttc taaacataat
    18721 ctaactaaag taaataagta taatagagtg gtagaaaagt tgcaatctaa attattaaga
    18781 gagccaactt catatgaaat tgcaaaagaa ttaaaggttt cagaaaaaga acttcatgat
    18841 atcgaaagta atatagatat gttaaatatt gtatcattaa actatgtaat ctttgaagat
    18901 acaaatgaaa ctgtacaaga tgtaataagt gatagagaag aggaagctcc agaaaatata
    18961 atagaagaag aaaagttaga gattctatcc aaagcaatta gtaatttaaa tgaaagagaa
    19021 aaattaatac tttcactgta ctattatgaa gatttaaatc taaaggagat agggaaagtg
    19081 ctaggtgttt cagaatcaag agtttcacaa ctccacagaa aatcaataag aaacttaaga
    19141 aacaagataa aagaacttaa atattctata tagatggtga ttgataagat gatacaaatt
    19201 atattaatag tagtgtctat tttaataata gtaactatag ttataaattt acagaaggaa
    19261 tcaaaaaata aagataacag atactttgat aaaatatttg agcaagaata tgaagcaaat
    19321 gatagattaa taattgcaga aaaaagaaga ttctttttag acaaagtact tgcagaaaaa
    19381 agaattaata gtcatataaa agtttatgaa aattataacc taataaaggc taatgaagaa
    19441 agaaaggaaa acatatttat agaatcaaaa aaggaagtaa gacaagaaaa tgagttatct
    19501 cttaaagtga aacatcttat aagttcaggt tatgatgatg tggaaatatg taagatgcta
    19561 gatattggaa agggggaact gttattaata aggagttgtt acaaaatata aggatattat
    19621 tatgtgataa aaaagtattc ttaggaatag gtattggtat attaatatca actttatgta
    19681 taatgctttt tagtaataat gatatgagta aggcagagat agaaaaaaag gccagagcgt
    19741 taggcatgaa atatacaagt gagatgaaag ttttagaata gatgatactg tttagacatt
    19801 tacaaaaaag gaggtcataa tgtgataaga ggattatata cagcagtttc tgctatgata
    19861 actaatcaac aaaagcaaaa tgttgttgtc aataacctag ctaatatgga tacaaatgga
    19921 tataaaagta aacaactttt gactaaaagt tttgatgaat taaagttgga gggttacaat
    19981 aactacgcaa atggaactaa acaaaagcag attataggcg atataagtcc aggtatttct
    20041 atggatgaaa ctatcacaaa ttttaatcaa ggacctataa aaactactga taataaaatg
    20101 gatgtagcta ttcaaggtaa gggatttttt ctagttagtg atgcagctgg aaatcaattt
    20161 tacactagaa atgggaactt cagaacagac aatcaaggtg accttataac aagtgaaggg
    20221 tattatgtac ttgggacgaa tactgctact ggtgctgtag gtcatataaa tgtaggaaat
    20281 caaaaatttg aagtggcaaa agataataca atatcactag acggaaatgc tatgtataaa
    20341 tttaatattg tagattttaa taactacaat aacttaaaaa aagaaggaga taacttatat
    20401 tctggaggag gagctatgaa agtaaataat gcacttacaa tacaaagtgc gatagagtcc
    20461 tctaatgtag atatggtatc tgaaattaat aatatgatga caatatcaag agaatatgag
    20521 gcaaatcaaa agataattca ggctatggat tctaaattag caaaaatagc aagtgagatt
    20581 ggttcagtaa gataggtaat aggaggtgaa tttttatgta cacaatgttt tatactagta
    20641 aaacagcttt atcagcaaat caaagtaaac tagatataat atctaacaat atagcaaatt
    20701 ctgattcaac aggatataaa aaaataaata tggaatattc tgatttagtg gatgaagtac
    20761 taaatagacc atcataccct acaaatggta aagatatatc gactggaaca ggagctaagg
    20821 caacagctcc catgagaaat tatgaacaag gtgcattggc tcctacagat agtaagagta
    20881 atttggcgat agatggagaa ggattcttta gagtgataag aaaagatggt acatatgcat
    20941 atacaagaaa tggtggattt aatgtagatg ctttaggtaa gattgtagat gataatggaa
    21001 atatattaga tgtccaattt gatgcaggat ataactataa taatacaaat atagatagta
    21061 caaacctgac agtttcaaga aatggagagc tattcgtaga taataaaaaa ataggaaaaa
    21121 ttaacttata tcaacctata ggaactcaaa attttatatc tgaaggagat agtttatttg
    21181 tcgcaactga tgcagcacag attaaacaag ttgagaaagt cgatatagtt caaggctata
    21241 gagagaggtc aaatgtttct ttacaagaag aatttgttga cttaatagcc actcaaagag
    21301 catttcaaat gaactcaaag ggaataaaga cagcagatga aatgtggcaa atagcgaata
    21361 atttaagagc taaataatta tgtaaggaga ggaatattca tgaatgatat gaaagagtac
    21421 gattttggca aacctcagag ttattcaaga gaaaatttaa cttgtttaaa ttttttatta
    21481 gctgaatttt gtaaaaaata taaaaactat ataatttatg aacttaagtg taattctagt
    21541 atggttgttg atagtataga ccaagtaaat tatcagactt ttttagatag agtaacttct
    21601 tcatgcgtag tagtacaaaa tgcaatggaa cctgttatga aaaacttttc ttttagaata
    21661 gatagaagtg tagctgatat gtggattgat ataacttctg gaggaactgg agttgtaaag
    21721 gataatgata gagagcttac agaacttgat aaaaaagtgc ttttacacct tatggatgat
    21781 ttagtaagaa atatgcactt atttgagggt tttgaaaata tacaagtatt ggatacacat
    21841 acacatatta atttgccaca attatgttct ccaactgcac cagtatgcgt tgtagattta
    21901 aaagtgctaa atgataatga gtgtattgga agtgtttctc tttgttttcc atacaatggt
    21961 ttagaggctc tgttagaaag tatatctgtt atagaatttt ttgataatga tgtaggagat
    22021 gattcagagg aatttaccca aaaaatctat aatagtgtat ctaatataga attatcagtt
    22081 attgcagaga taggaaaagt atcaattaat gttgaggatt tacttaaact agaagttgga
    22141 gatgtaatag taacaaataa aaaaataaat gattatatag atatatttgt agaaaattca
    22201 aaatcataca cagcaacacc aggatttata tcaagtaaaa aaggtgtaaa aataaatgat
    22261 gctgttggaa aagaggtgta gttatggaca atagtaatct tatttcagat gaagaagcaa
    22321 agatgttatt aaatgataaa gttgaaagtg aagttgatgg agatgatgag ttagccttag
    22381 atgtctctga agttaatcga aataatatac aaaggatact tgaccttaag ttagaattat
    22441 ctgtagttat aggaagaact aagatgactc taagagatat tttaaattta cataaaggtt
    22501 ctcttattga actggatact ttagcagacc aagatgtgga aatactaata gataataagg
    22561 ttttggctta tggaaaagta gtggtggtag atttaaattt tggtgtaaag ataacaagta
    22621 tagtaagtga agaagacatg gtaaaatctc ttgtttagag ataacatcaa ttaatactta
    22681 aggcatgagg tggtacttat tttaaactta aatgatataa aaaaagatat actaaaactc
    22741 aataaaaaag actcatgttc tctatataat gaatctatta ataatataaa taataattgt
    22801 atatcaactg gattaaaatt tttaaaacaa gcaagagatt taaacccaga tgatgttgat
    22861 atattaaatc tcacaggtct tgtaaactta ctaaaatgta attttgatga tgctgtagaa
    22921 agtttctata agagttcact ttgtgaaaaa actgctctgt gtgaaaagta tataaacatg
    22981 cttacatctg aggagttttt aattttcttt gaaaagtaca atcaacttat aaggttaata
    23041 aataaaggaa tgtataaaga agctatacaa gggtttaaac tcataactga ccaatactgt
    23101 gatttaatag aaccttatga gttgcttgcc ttactgtatg acaaggaaga agaatatata
    23161 aaatttgatg aatgtttaga agtattgaag attattgata aggaaaatta tttattaaat
    23221 caagaaaata acacattaga tatgtataat agtgctaata tacaaactaa aagtaatata
    23281 aaagtagatg attataagaa tgagttctta cataaaagta gtagtaacca taaaattaat
    23341 gattataatg atgtaactaa agaaaagagt tcaagcaaaa accagaaaag ttcagattct
    23401 aataagaaaa aaagtattaa aaaaaataag tacctatata tagccataat attaggaata
    23461 ttattagtag gacaaagtat atataattct tataagatga ataatttaac tgataaaata
    23521 tctaaaaata atgaaactaa aaatgaagag tcaatagatt ctaacttgac taagaaagaa
    23581 aataatatag aagaaaatga agatatgagt aaggcttcta atataaataa gagtaataaa
    23641 aacattaata aagaggatga tgtaaaatct aaaaaaaata ctgtagaaaa tgaagatgat
    23701 aaagattctg acttatttac agaggatgaa ttgatgtcta aggcgaatac tttaaaatca
    23761 gaaaagaaaa ctaaatcttc tatacaacta tataagaaag ttgcagatat aggtgaaaat
    23821 aaagggaata catctgaagc aacttatcaa gttgctattc tcagtgaaaa gttaaaagac
    23881 tataaaactg ctgaagaata ttataaaatg tatgtagaaa attactcaga aaaagatgct
    23941 tattttgatg aatcttatta taatttaggt atgatgtact ataacaatgg tgatttaaaa
    24001 aattctaaat tgacattaaa gaagttggtt aataaggtac caaatagtat gtataataat
    24061 tcaaaagtaa aagaaatttt aaaagaagaa tagttaaatt agataagaaa aataaattat
    24121 tttattttaa aataatatga ttataatata ttgacatacc attttaaaaa atttaaaata
    24181 ttaattataa aatgaggagg gataaaaaat ggaatttgaa aaagggagta tttcaattca
    24241 tacagaaaat atttttccta taattaaaaa atggttatac tctgataaag acatatttat
    24301 aagagagctt ataagtaatg gatgtgatgc agtaagtaaa cataaaagac ttgtatcact
    24361 aggagaaata tcagaaaata agtcatcaga ttataaaatt acagtatctg taaataaagg
    24421 agaaggtaca cttaaattta ttgacaatgg aattggtatg actgaagaag agataaaaaa
    24481 atacataaat caagttgctt tttcaggtgc tgaagatttc tttaataaat ataaggataa
    24541 aatggaagag tcaaatgata taataggtca ttttggatta ggattttatt ctgcatttat
    24601 ggtgtctaaa aaggtacaaa tagatacact ttcatataca gaaggagcga ctcctgtaag
    24661 atggatatct gaaggtggta cagaatacga aatatctgaa tcagatgcta gaaatgatag
    24721 aggaactact ataacactat ttattgatga tgatagtaaa gaatttttag atgaatttac
    24781 tgtaagagga ataataaata aatactgttc tttcttacca gtagaaatat atttagaaga
    24841 tgttgaaaga ttagaaagag aagctaaaga agcagaagaa aaagctaaaa aacaaaagga
    24901 agatggaaaa gaagagattg tagatgcaaa agtaattgag cctttaaacg acactaatcc
    24961 tctatggtta aaatctccaa aagactgtac tgatgaggag tataaagagt tttatagaaa
    25021 ggtattcaat gtatttgatg aacctttatt ttggatacat ttaaatgtag attatccttt
    25081 caatttaaag ggtatacttt acttccctaa attaaaaaat gagtttgaac tgactgaagg
    25141 aaaagtaaaa ttatacaaca atcaagtgtt tgtggctgac aatataaaag aagttatacc
    25201 agaattttta cttttattaa aaggagttat agactgccca gacttaccat taaatgtatc
    25261 aagaagtttc ttacaaaatg acagggatgt aagcaaaatt tctaaacata taattaaaaa
    25321 agtagctgat aagttaaaat ctctttgtaa aaatgaaaga gaagaataca ataagttttg
    25381 ggatgatata caaatattta ttaaatatgg ttgcttaaaa gatgaaagtt tctatgagaa
    25441 ggtaaaagaa tgtatattat tcaaaactat tgatgatgag tatataacat tacaagatta
    25501 tttagaaaag tgtaaggaca aacatgaaaa taaggtattt tatgtaagtg ataaagaaca
    25561 gcaatctcaa tacattaaac tatttaaaga atacgactta agtgctgttg ttttaaactc
    25621 atcaatagat actcatttta tttcatttat ggaatataaa gagaatggag ttaagtttaa
    25681 tagaatagat gcagaccttt ctgatgtatt aaaagataaa aatgaaaata aagatagtga
    25741 agaaaacaaa gaagagatag ctaagataga aggtctgttt aaagaagctg ttggagaaag
    25801 agtgaagaat tattctgtag aaggattgaa aaatgaagac actccagcta tggtgttagt
    25861 atcagaacaa tctattagaa tggcagaaat gcagtcaaga tttgctggaa tggatttagg
    25921 aatgaatttt gaagaagaaa aaactcttgt aataaatgag aacagtccaa taatcaaaaa
    25981 attggtttct cttaaagatg atgaggaaaa gaaagataag attacgctta tttgtaacca
    26041 gatagcagat ttagctttac tttctaataa agaattaaag ccagatgagt tagattcatt
    26101 tgtacaaaga agtaataagc ttatgagtct tttgatagaa ttataagatt gaatttgaat
    26161 acattgaagc aaatttataa aaagtattca atatcagtga taagaaagaa gtgtctcaaa
    26221 atgaactttt cagttcatga ggcgcttttt ttatgtgaaa ttaaatttat tttaatcatt
    26281 tcaacaatga aaaatatatt tatctctatt ttgagacaga ccgttcttta tttttttatc
    26341 tttggttaat ttttaaatat ttaagtaggt tctaataaaa aactctattt agttaaatat
    26401 agttaacttg atatataata agataataca tataattaag taaaagattg ttttttagtt
    26461 tatgtttaaa tacataatta cactttgtta aaaacataaa attattacta aagtagaaat
    26521 aggaggtttt agcatgaatt ttaattataa tttgccagta aatatcttat ttgggagcgg
    26581 cagaattaat gatgtgggaa aagaagtatc taaatatggc aaaaaagtat tgatagtaac
    26641 aggtaaaaat agtacaaaga aaactggatt acttgataaa acaatagatt tactaaaaga
    26701 ctctaaaata gaatatgaag tttttgatag agtagaacaa aatcctctta taactactat
    26761 atattcagga gtagaaatta taaaatcaac tggatgtgat tgtgttttag gtcttggtgg
    26821 tggaagcatt atggatgctg ccaagagtat agctttttct tataaaaacc caggagattt
    26881 aaatgattat atatttggaa ttagacaagg tgaagaagct ttacctataa tcttagttcc
    26941 aacaacatca ggtacaggta gtgaaggaaa ttgttttgca gtacttacaa attcagaaac
    27001 taaagataaa aaatctataa agaagaatag tatgtatgca aaagcatcaa taattgaccc
    27061 agaacttatg gttacaatgc caaaacatat aattgcatca gttggatttg atgcacttgc
    27121 ccataatatg gaagcatatc tttctaatgg aagaaaccct cttgcagatg tacaagctat
    27181 ttatgggatt gaacttatat cagaaaacct aataaaggta tataatgatg ttaatgattt
    27241 agaagcatgg gaaaaaataa cacttgcaag tactattgga ggaatgacga ttggtacagc
    27301 gggtacagcc ttgccacatg gaatggagca tcctgtaagt ggtcttagag atgtaatgca
    27361 tggaaaagga cttgcagctt tgactcctac tattatagaa agttcatggg atagtgatat
    27421 tgaaagattt ggtaatattt caaaggtatt aggaggaact tgtgcagaag attgttcaga
    27481 tgcaattaga aatttcctta aaaaaataga tttagatata aagctaagtg atttaggtat
    27541 tgaaaaatca gatatagatt ggttaactga taattgtatg aaaatatcag ttgcaaatat
    27601 taagagacat ccaaagtatt ttaataagga acaaataaag gaaatgtacc ataagtcttt
    27661 ataatttgac tttacatatt tagttttata taaaaattat aaattaataa taaaaaatta
    27721 taagtaatta atagt
//
LOCUS       DAYAPA010000045        27687 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.045, whole genome shotgun sequence.
ACCESSION   DAYAPA010000045 DAYAPA010000000
VERSION     DAYAPA010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27687
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.045"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(<1..425)
                     /locus_tag="ACGI59_001778"
     CDS             complement(<1..425)
                     /locus_tag="ACGI59_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9739564.1"
                     /translation="MIGCIYEVYNEEITKIIENQNTLAVYLVGSSKDVDFTLYDVEIN
                     DIDVFVFVKEGEKQERILFKKKGIEFDVNYFSKDGVKKLLSNREYFFVKEMKDAKVLF
                     DRENISHIIKDISRNIYLEGPSKMSLEEKSFIKQDIGAK"
     gene            complement(459..1202)
                     /locus_tag="ACGI59_001779"
     CDS             complement(459..1202)
                     /locus_tag="ACGI59_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HFG9739565.1"
                     /translation="MKNNVGFDSSLSKFGIGLFETIRVKNGIAVDLDIHIERMLSSIN
                     CLDLDIKYKKDFLINEIVTYIKKENVINKALRITVFDEGYNISIRDIPYNQETYEKGF
                     KLAISPIVRGNSLIHRHKTTNYFENIYTKNIANKTGYNDGIFLNSEGVILECSMSNIF
                     FVKGERVYTPSDRLPILNGIIKKRIIEICDELHIELIENEINISEISSFDFVFVTNSL
                     MGAIKVTEIDKIKFNKENVVFDKIVKLLE"
     gene            complement(1192..2541)
                     /gene="pabB"
                     /locus_tag="ACGI59_001780"
     CDS             complement(1192..2541)
                     /gene="pabB"
                     /locus_tag="ACGI59_001780"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008153 - para-aminobenzoic acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HFG9739566.1"
                     /translation="MIREINTKLNSFEIFTIFRNEHDSFILDSAMDKEKLGRYSFISS
                     QPFKVLKYKDTDENPLEVLKEELHKYRVVNDTNLPFVGGAVGYLSYDLGNYIENLPRT
                     AVDDIEMPDMYFGFYNHVIVIDHLVQKTYIATPNIDIELEEKIIDDIEQRILKEEKKG
                     IDSICYEEKEVTPIRLKSNFTKEEFKNAVQSVREYIRQGDIYQANLTQRFSGETELTS
                     FELYRDLRRFSPAPFGAFLNFEDAHILSNSPERFIRCVNKRIETRPIKGTRPRGKDKE
                     EDLRLQQELRNSEKDRAELLMIVDLERNDIGRISKTGSVKVPELFVIEPYANVNHLVS
                     TVVGELKDDKDATDVIKATFPGGSITGAPKIRAMEIIDELEPTQRNVYTGSIGYIGFN
                     GDMDFNIAIRTIIKNDKKVYFQVGGGMTWDSDPDEEYQETLDKAKSIMKALRGYYEE"
     gene            complement(2543..3127)
                     /locus_tag="ACGI59_001781"
     CDS             complement(2543..3127)
                     /locus_tag="ACGI59_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HFG9739567.1"
                     /translation="MILMIDNYDSFVYNLVQYIEELGETVVVKRNNEIKISDIEELNP
                     EVIVLSPGPCSPKEAGICIDIVEHFKGKKPILGICLGHQTIGHVFGGDIIKAQQPVHG
                     KVYSINHTNKGVFRGLKNPLNVTRYHSLIVDSNTVPKELEITAITDKGEIMGIRHKKY
                     LIEGVQFHPEAILSEYGHEMLKNFITEARERVHM"
     gene            complement(3151..3870)
                     /locus_tag="ACGI59_001782"
     CDS             complement(3151..3870)
                     /locus_tag="ACGI59_001782"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFG9739568.1"
                     /translation="MTSWKKKTVYKCLIAVVLFCGIVLISNFSKVSALMMDTNGNVLI
                     KHGSREKKLIAITFDDGPHPKETSQVLDVLKKYNVKATFFIAGKHAKWYKEPLVRASK
                     EGHEIGNHTFNHPDISNLSSSQIEEEIVKCEDILKEVTGKKPTLFRPPFGSYREKELI
                     EIAKKHDYKVVLWTGVDVKDWKNPGANSIADKIINKVQNGDIILLHDYATNDTVEALD
                     MFIPKMIEKGFKFVTVSELIK"
     gene            complement(3898..4962)
                     /locus_tag="ACGI59_001783"
     CDS             complement(3898..4962)
                     /locus_tag="ACGI59_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HFG9739569.1"
                     /translation="MKRLIALVLVVLIVLFYSPELLKEGYDLKEKFFQSEIWNEFLDK
                     ININDNSKKDKKEKSQNLQSNNTEGENLGESDIKNFEKISLGDNLSYVLSSIGKPGRI
                     DISEYGFDWYVYNQYGKEFAMVGLENDEVVALYSNSINSCENQDIKLNQDRQTVRTKI
                     TPLKYKRKGNTRYIINSENQYDIISKEGKYITIFYDIHEENRVCSYLIIDKSTEDEFK
                     NLYPDDSEELKKCFELEVIDLVNSVRNQRGLNSLRYSEQATLSSRKHSEDMRDNNFFD
                     HVNKKNETPFDRMKREGIVYTSAGENIAAGQINAIYAHEAWMNSEGHRKNILGNYNNI
                     GVGVIFGGSYKTYYTQNFYK"
     gene            complement(5106..5915)
                     /locus_tag="ACGI59_001784"
     CDS             complement(5106..5915)
                     /locus_tag="ACGI59_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MORN repeat-containing protein"
                     /protein_id="HFG9739570.1"
                     /translation="MNLSKEYVNLYDNYLKYINEVNKEIRSIKNMKKRVLKRGDFAPE
                     NEVLELEGKTIRDIDDVDTKKPKNKIYKFSNGDVYVGKFLEGKMEGKGSYTFFVDEGT
                     VMEYIGEFKEDKKNGKGNFIFSNGNEYIGYFEEDMMSGIGNMLYSSKDEYIGTWKNGK
                     KDGKGIYKWSDGCIYAGEFKGGKMEGYGICYNSKGEVLYEGEWKNNLIHGKGTYIWEK
                     GKKYIGEFMHGKKHGQGTFYLNNELVYEGTWKFDKPSIFDRTLDEIFSLRL"
     gene            complement(6157..7023)
                     /locus_tag="ACGI59_001785"
     CDS             complement(6157..7023)
                     /locus_tag="ACGI59_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFG9739571.1"
                     /translation="MSEVKNTLQKHPVLRYSTMLFGMLMTSIGINGFLRPAHLLSGGA
                     TGIATSINYLTNINVGLLTFLINIPIFILGFIYLEKEFCISSLVNMIVFSLLLGATQE
                     ISNIIPIHDILLQSVYGGILSGLGVGVVFRTRSSQGGTDIIAAILKIKKNIEMKDTAL
                     AINGLIVLTGSFLFGLDLALYTLIGLFLNAYSMSFIKDAMNYQKSVMVMSNEVDLIAE
                     DIMKSLVRGVTFLDAEGAYTHQKKKIIYTIVSSNEIPKIKDIALKYDKKAFISVNDVT
                     EVKGRGFKAKDL"
     gene            complement(7543..8232)
                     /locus_tag="ACGI59_001786"
     CDS             complement(7543..8232)
                     /locus_tag="ACGI59_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HFG9739572.1"
                     /translation="MYNVLQTPVFGIIVTIVFFNLGRYIQRKTSNPICNPLLIAIVGI
                     ILFLSISKIPYENYKIGADSLNFFLGPVTVVLAVPLYKQFELFKKHMFEIIVGIGCGI
                     VISFVSVLIIGKIANSDVSIINSLIPKSITTPMGISLANSLNGVESITVVAIIFTGIF
                     GGMVASTVFKLGNINHPVAKGIALGTSAHALGTTKAFELGEIEGAMSGVSIGVSGTIT
                     VILIPIIMNFI"
     gene            complement(8225..8596)
                     /locus_tag="ACGI59_001787"
     CDS             complement(8225..8596)
                     /locus_tag="ACGI59_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFG9739573.1"
                     /translation="MKVFNQLVIILSIWVIGEYISSFIQGIILIPGSIVGMLLLFLLL
                     QFKVLDLSSIENVSGFFLDNMAIFFIPAGVSLIKSLDLIRENVLVLLLVICLSTLIVM
                     YTTGIIVEKMIKKKEEGQKNV"
     gene            complement(8778..10175)
                     /locus_tag="ACGI59_001788"
     CDS             complement(8778..10175)
                     /locus_tag="ACGI59_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFG9739574.1"
                     /translation="MEVSIILVKDIKQDIESMPRKILDISLIEDNKSDLTFFNIEEED
                     GDETLKALKQSYVTTNYKEETLLNLQGALNSKSDTTSKELKLESYKRYNNALDLAYKN
                     YITSAINMVNKALEINPKDIDILNLRGLLKLLKCDFAKSFESFYTALCYENNHISRKY
                     VNLISSEDFKTFLGRYNHSIRFINEELNYESIHILENIIEEEPELIEPYVILSLLYDK
                     LGNVKKREGYLDKLKELDKDNPVFEKNEEEKEDTSKKEEIKKVKRKKSILPYIIVGCV
                     LIGMGIYLIQSKKRIENLNNQISSKEEKLSETDKKLGETSKKLEKTNKELNEVKNKEP
                     EREITVADEEDLYYQALNLKKNKEYEKAIDNFKSVVSSGKTKKYISESIYQLAITNKL
                     LGNKDEAIKYYKKYINTYTKNDQYYDDSYYELGMLYYDNGDLKNAQQTFYSLRSEVPD
                     SMYNNSKIKEILSEK"
     gene            complement(10306..10899)
                     /locus_tag="ACGI59_001789"
     CDS             complement(10306..10899)
                     /locus_tag="ACGI59_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFG9739575.1"
                     /translation="MNLNNITNYVQSICENISNVLDVDVTLVTKDLTRIAGTGIFRGK
                     IGETISEKSVYSLVLKDGKSYVINKEIEENCNKCANRENCKELADICTPVKMGEHILG
                     ILGIAAFNEEQKEKILSKDKELTAFVRSMSDLISFKLDELYKQEVKTIDDLEREAIEN
                     AIKKYGSNTEGMKKVAEALNIGIATLYRKVKKFNIKS"
     gene            complement(10964..12433)
                     /locus_tag="ACGI59_001790"
     CDS             complement(10964..12433)
                     /locus_tag="ACGI59_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HFG9739576.1"
                     /translation="MSNVNKKLVIAIGAVALVVIIFIGIMCMQFKGEKIAKNTYVNGV
                     NIGKLTKSQAKQELAKKYKLENVDFNYNDKSWKVKSKDLNLSTDLDKTVENAYNLNRK
                     SAFFGNLSKTISANFGKKSNLVVAINYDKNKLKAEMEKIAKEIDVDVKDATLDISGEK
                     VKVIPDSDGLKMDISKSMENFDNQTKKGNYKNELVVKATPAKVKKEQLANIDTNLGTY
                     STTFKTSQINRSINIKLATDNISNVLLMPGETFSFNKHTGKRSKENGYKSAPVIMEGE
                     MEEDYGGGVCQVSSTLYNSVLYAGLEIVNVKNHTIPSSYVPKGRDATVADSGIDFLFK
                     NNLKHPVYIKNYVSGNQIVCNIYGSAEDKQNITISTKLDGVSQTTMKRVNDPTMPKGK
                     EKVDKSGRNAYSVSTYRTFNDANGKKIKTEKIANSYYPKKEGIILVGTMEPKPEEKPN
                     TDENKNNQNTNNQNPNNQQKPETPPTDNKPNETQPQPQA"
     gene            complement(12548..13939)
                     /locus_tag="ACGI59_001791"
     CDS             complement(12548..13939)
                     /locus_tag="ACGI59_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HFG9739577.1"
                     /translation="MNFRDLFEKAVDFIDEKRKSIVAISLSTLGVIALIIVFFLSSNE
                     FSVGNEANELLKIIEKRQYSIAVDYYTSIEKKFSDSKMERFNKSVSKKINKLLLNSGD
                     KYLDGDISQESFIGLINTVKELNKISIDVDDLLAQADRVREMYKEENTTYDIAINYIS
                     TVSILNGMGSNLDVYKQNIETIKESRDVYDSAVKDQKAYKYYEAIEKYNKVLKEDEKY
                     YSMAQNGKEQCIEEMYDYYISRAEEANTSGDYERALQYIEYLKDDYSDDEKVQSLEKK
                     YKKNLSLYTLTSDDIINVIAKKSGKDKANLSINSLPQMIKNNKYYYAEVYEYDKLINE
                     VLVSAKTKDLYSYKDGKKDYKVDYGDGYFRILEDGSYQFGITKDKAKFVLTNTLDEKE
                     NKYKKIEILDIEKADRYVKSKKSLEELFGKYKNIYYYAVVNKGLFRGKEVYAINIYNE
                     KIYAISENGLNEY"
     gene            complement(14105..14395)
                     /locus_tag="ACGI59_001792"
     CDS             complement(14105..14395)
                     /locus_tag="ACGI59_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9739578.1"
                     /translation="MSGLKKILRILVISMMICFAMTFALVLINSVMGVHIGLDYYFLM
                     GSVLTVCLTIGIVLCNARKIFDSKAIHKTRTKTVSNNKKQKSHSRDIKRKIS"
     gene            14619..16748
                     /locus_tag="ACGI59_001793"
     CDS             14619..16748
                     /locus_tag="ACGI59_001793"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HFG9739579.1"
                     /translation="MPNLPSLGSKAPDFKANTTNGPIRLSDYKGNWIVLFSHPGDFTP
                     VCTTEFLCFAKYYDEFKKRNTELIGLSVDSNSSHLAWMYNISLLTGVEIPFPIIEDRD
                     MRIAKLYGMISKPMSDTSTVRSVFIIDNNQILRTILYYPLTTGRNIPEILRIVDALQT
                     SDRDNIVTPANWFPGMPVILPYPKNYKELKNRVNSCNKKYSCMDWYLCFVPDNYNDEE
                     VSKKIDNTCSWKKEHTKNIENECNCEHEHHDYLNKALDCKQEHKTDIKDDCNHEKKHT
                     KNTNKVHNSKQDKFKDKSCDEMNFNYDKDESCDKINSSYNKEDSSYEDFYKHNYKNYD
                     YTSEKNTKKIAMKTLKDSKKLVRPQITDPYNPIVENANCPDINPIVAEYVLGNPTNVD
                     AQLLDAVIFAFAEIDQSGNLFIPYPRFLNQLLALKGEKPSLKVIVAIGGWGAEGFSDA
                     ALTPTSRYNFARQVNQMINEYALDGIDIDWEYPGSSASGITSRPQDRENFTLLLTAIR
                     DVIGDDKWLSVAGTGDRGYINSSAEIDKIAPIIDYFNLMSYDFTAGETGPNGRKHQAN
                     LFDSDLSLPGYSVDAMVRNLENAGMPSEKILLGIPFYGRLGATITRTYDELRRDYINK
                     NGYEYRFDNTAQVPYLVKDGDFAMSYDDALSIFLKTQYVLRNCLGGVFSWTSTYDQAN
                     ILARTMSIGINDPEVLKEELEGIYGQF"
     gene            complement(16975..17574)
                     /locus_tag="ACGI59_001794"
     CDS             complement(16975..17574)
                     /locus_tag="ACGI59_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viroplasmin family protein"
                     /protein_id="HFG9739580.1"
                     /translation="MAKQKFYAVKKGKNIGVYNTWDECKKQVNGFSGAEYKSFSTFQE
                     AKEYIDGSEKLSFQEDKEFIEAYVDGSYEHSVKMYGSGVVILKNNEVIKTYSEKGKEK
                     TLVSMRNVAGEIEASKIAMQYCIDNNVQNLILYFDYEGIEKWCTGVWKTNKEGTIAYK
                     NFYDSIKNKLNVKFTKVKAHSGNKYNEEADKLAKKAIGV"
     gene            complement(17592..20711)
                     /locus_tag="ACGI59_001795"
     CDS             complement(17592..20711)
                     /locus_tag="ACGI59_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HFG9739581.1"
                     /translation="MDNIISVLNDVVFLDIEVSGLDCLNSEILEVGAVKVKDWKIYTY
                     ESLIKNKFEVPIEVFSVCKNLDKNDLEIANKIELVEDRLVNFVEDSFIICHDLSLKKK
                     FFEYHMPKLKNKFIDLIELAVILEPYHKDYSLEYLKNTLTNCNSKVENRALSDAIDII
                     NIVNCLLVKFNNYEKTTLEPLSFKINSYLKKFNLPTWEWSKFLEEANYDLSNNINIKK
                     EYNIFDSKEEKKKERETLKILNEEEKNYEELLKYKTIWENKEGFTYEYRPGQYELTKT
                     IRELFRNSEDEEKIACIEAPTGIGKSVGYLLPAILEARINKKRLLISTDTKELQIQLI
                     NKDIPNVLDSLGLNGKVSYGYIKGKNNYICIDRLEAYIDDYESQNPTKGEILSLIFLK
                     RLVEGGKYGDIEEINYLVFDNFKEISTHLRNVSCDPNMCRPKKCKKDCLYKNRIEELK
                     EEHITVVNHSLLAKWPYKDEKPLENIIVDEAHNLTEKGYDFFSSIINSKSLRYLLQEI
                     YPYEFIQNSSFIYKKYSRNMRKIKAFDKFYNVLKIGREDKQKIARSINFIIEEIDSIL
                     KFGNCNEYNNVSNYNLRWELNLQIDEIVGKLKKDGIDTEISYRAYSEKIKLSCEKIIK
                     NLVSIIIIIYRNIDDDSIDKEADIYKFGKAKTRDLEDIKIIFEIFLEYDEKDDYARIV
                     EIDKNYNDFEFRVVPLKIADLFEENILSQLEKGIFLSATLSLSESMSYFKNTLGIDRV
                     KNVEKIIEPIFDYKNRVSVVGFSDICEYRNSEFPNEMSKIISNISKITEGHTLALFNS
                     KDRQEKTYEILKKYLHSFNLEIYADKKGIRHLNDLNRKCVVLGSKGCFEGVDIPGDGL
                     VCVTLDKLPNLNPKDPLYFTIMKKYGIDYYTINYPQMTIKVKQAMGRILRSKYDYGCF
                     VIFDVGTNISVLKRLEKDLHDCKISKVNSNEFYTYIRRHLNKSRSLILKSVIFDTIKA
                     LNVDAKMDNNDVDKDIIKKDINENIRQRAVKGEVYHIDIIKKDMKVKYFDRNYLINLD
                     IFMREEDKN"
     gene            20932..21756
                     /gene="pdaA"
                     /locus_tag="ACGI59_001796"
     CDS             20932..21756
                     /gene="pdaA"
                     /locus_tag="ACGI59_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="delta-lactam-biosynthetic de-N-acetylase"
                     /protein_id="HFG9739582.1"
                     /translation="MKKDSLKKYIMIGAFALTLFGIASINFKSLDKTKTQISSPTLDT
                     HEYDWYFNPREDGKQPSPIKEADFFKKYGAYYVGNPNEKVIYLSFDAGYESGNTPKLL
                     DTLKKHNAKAQFFVVESYIKSNPELIKRMEKEGHLVCNHSKSHPSMAGITDFEKFKEE
                     ITSVEKAYKDVTGKEMPKYFRPPMGKFSEQSLKYTQDLGYKSIFWSFAYVDWYEKKQP
                     THEFAKNKIYSRTHPGAIVLLHPNSSTNTEILDEVLTHWEKEGYKLKTLDYLNNKK"
     gene            complement(21853..22638)
                     /locus_tag="ACGI59_001797"
     CDS             complement(21853..22638)
                     /locus_tag="ACGI59_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFG9739583.1"
                     /translation="MIVKIDNTLEKEFWQYVSHEESLNLFIIGYVENYGFSSQYQDIW
                     SQVEGGNITSIILKNKSTLIIYSFKNNFNIGEMKNHIKDLDVESISGKKCVIDRLISK
                     YKDFYEKLDNKFCVLKEIKEIDFSNMKEYKIENAQEKDIDEIGKLLNRSDYKVSKNYI
                     EERKVHLKEGNVRAYFIRNDDTMISTVSTGMETSFLAMVVSVSTDKRYRGKGLASYMV
                     YNLSKELLLEGKVPCLFYNNDVAGKIYHNIGYKEINEWTILFK"
     gene            complement(22738..22872)
                     /locus_tag="ACGI59_001798"
     CDS             complement(22738..22872)
                     /locus_tag="ACGI59_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9739584.1"
                     /translation="MNISRKAMKIIELAQKIANKRGISVEEAWSEAVTEYKNKYEHIA
                     "
     gene            23103..23468
                     /locus_tag="ACGI59_001799"
     CDS             23103..23468
                     /locus_tag="ACGI59_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFG9739585.1"
                     /translation="MKIRAEYTCPLEIVHDIIKGKWKTILIFELRQSNKTFSELQHNI
                     YGINQKMLIQQLKELRDFGIIDKISSAGYPLHVEYFLTNRGKKMLKAVEIMQEIGIEY
                     MLEHGQQEILDNKGICYNK"
     gene            23546..24100
                     /locus_tag="ACGI59_001800"
     CDS             23546..24100
                     /locus_tag="ACGI59_001800"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFG9739586.1"
                     /translation="MSIALILIDIQNDYFKNGKCELFQSEETAENAKKILLFFRKNNL
                     PVIHIQHISTDKTASFFLPNTHGSEINKIVFPLDNEEIIIKHTPDSFFETDLQSILEK
                     KNITNLVICGMMSHMCIDTSVRTAKKLRYDITLISDACTTKNLSWNNIEINANTVHQV
                     FMASLQNSFADVKNTDKFLSNYYK"
     gene            24320..24994
                     /locus_tag="ACGI59_001801"
     CDS             24320..24994
                     /locus_tag="ACGI59_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFG9739587.1"
                     /translation="MNHHILIQFEYIARIITAGFCGALIGYERKNRLKEAGIRTHFIV
                     ALASALIIIVSKYGFNDIVGTPGIGLDPARVAAQVVSGIGFLGAGLIFIRNQSVSGLT
                     TAAGIWATAGTGLAIGSGLYLVGIVSSIIIVIVQITLHRDRKWMKLPTAEQVSLKISN
                     STDSIEYIEGKFKKSNIEIINMNIKRLDGDWLEVVIYTKLPKGYKKTNLLKLFSDNPL
                     IETLEI"
     regulatory      25349..25445
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            25499..25675
                     /locus_tag="ACGI59_001802"
     CDS             25499..25675
                     /locus_tag="ACGI59_001802"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9739588.1"
                     /translation="MFRDEMDKCTHMLTAYISSLYDYCDFIDTQLDDFILEYGENVVE
                     SCLHQVMVLVSKYN"
     gene            complement(25846..26085)
                     /locus_tag="ACGI59_001803"
     CDS             complement(25846..26085)
                     /locus_tag="ACGI59_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9739589.1"
                     /translation="MKCSNCGSVNIEKGILTTGGIYAETIGLKYRGDGKLDKVINKTF
                     PVQSTIYSDLCLDCGEIVRTYIKDNTDRNWCKDKK"
     gene            complement(26200..27039)
                     /locus_tag="ACGI59_001804"
     CDS             complement(26200..27039)
                     /locus_tag="ACGI59_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFG9739590.1"
                     /translation="MKDYYKIGEISKIYGIGRDSLMYYEEIGILRPVRDINGYRMYNI
                     SDIWKLNLIKEFRSLNFPMKKIKEYLDDRSIESTKNILNEELDLIDKKIAEFISHKEN
                     IIKRLSSIESVIQNTKIDEIEVVYIEKRKALELNADIKRDEDFDFLIQKLQKEYEDRF
                     NILGNNNIGSAFSTEAINKGIFNEFKSVFCFLESNEKVYNIVFDEGYYVTLNYTGSNS
                     NNKMYMEKVFKFIEENNYKIIADPIEIYKIDIHETGIVEEFVTEIQVPITKYLYFNGL
                     DGK"
BASE COUNT         9190 a         4519 c         2765 g        11213 t
ORIGIN      
        1 atttttgctc caatatcttg ctttataaaa gacttttcct ctaatgacat tttactgggt
       61 ccttctaagt aaatatttct acttatatct tttataatat gtgatatatt ctccctatca
      121 aacagtactt tagcatcttt catttctttc acaaagaaat attccctatt tgataataat
      181 ttttttacac catcttttga aaagtaattt acatcaaatt ctattccctt ttttttaaac
      241 agtattctct cttgtttttc cccttctttt acaaaaacga acacatcaat atcgtttatc
      301 tctacatcat aaagagtaaa atctacgtct ttactagaac ccactaaata tacagccaaa
      361 gtgttttggt tttctattat tttagttatc tcttcattgt atacttcata aatacaacct
      421 atcatctttt gtctccaaca aattgttaat attttaattt attctaataa cttcactatt
      481 ttatcaaaaa caacattttc tttattaaat tttattttgt caatttctgt aacctttata
      541 gcgcccataa gactatttgt gacaaaaaca aaatcaaaac tactaatttc tgatatatta
      601 atttcatttt ctatcaactc tatatgtaat tcgtcacaaa tctctataat cctttttttt
      661 attattccat ttaatatcgg taatctatca ctaggagtgt acactctttc tccttttaca
      721 aagaaaatat tgctcataga acattctaat attacacctt ctgaatttaa aaatatacca
      781 tcattataac cagtcttatt tgcgatattt ttggtatata tgttttcaaa ataattagta
      841 gtcttatgtc tatgaattaa actattcccc ctaactattg gagatatagc taatttaaac
      901 cccttctcat acgtctcttg attatatgga atatccctaa tagatatatt ataaccttca
      961 tcaaaaacag taattctaag tgctttatta atgacatttt ctttttttat atatgtaaca
     1021 atttcattta tcaagaaatc ttttttatat tttatatcta aatccaaaca atttattgaa
     1081 cttagcattc tttctatatg gatatctaaa tctactgcta ttccattttt aactctaata
     1141 gtttcaaaca aaccaattcc aaatttactt aaactactat caaaacctac attattcttc
     1201 ataatatccc ctcaaagcct tcattatgga tttagcttta tcaagtgttt cttgatattc
     1261 ttcatctggg tcagaatccc aagtcattcc tcctccaact tgaaaataga cttttttgtc
     1321 atttttgatt attgttctaa ttgctatatt aaaatccata tcaccattaa aacctatata
     1381 acctattgaa ccagtatata catttctttg cgttggctct aattcgtcta taatttccat
     1441 tgctctaatc ttaggtgctc ctgttataga cccccctgga aaagttgctt ttattacatc
     1501 agtagcatct ttatcatctt ttaactcacc tacaactgtg gaaactaagt gatttacatt
     1561 tgcatatggt tcaattacaa atagctctgg aactttaaca cttccagttt tagatattct
     1621 cccaatatca tttctttcta aatctactat cataagtagt tcagctctat ctttttcact
     1681 atttctaagt tcttgttgaa gtcttaaatc ttcttcttta tctttccctc ttggacgagt
     1741 accttttatt ggtctagttt ctattctctt attaacacat cttataaatc tttctggaga
     1801 atttgataat atatgtgcat cttcaaagtt taagaatgcc ccaaatggtg ctgggctaaa
     1861 tcttcttaag tccctataca attcaaagct tgtaagttct gtttctccac tgaacctttg
     1921 tgttaaatta gcttgatata tatctccttg tcttatgtac tctctaacac tttgaactgc
     1981 atttttaaat tcttcctttg taaaattaga tttaagtctt ataggtgtta cttctttttc
     2041 ttcataacat atgctatcta tacctttttt ctcttctttt aatattcttt gttctatatc
     2101 atctattatc ttttcttcta attcgatatc tatgtttgga gtagctatat aagttttttg
     2161 cacaagatgg tctataacta ttacatgatt ataaaatcca aaatacatat caggcatctc
     2221 aatatcatct acagcagttc taggtaaatt ttctatgtaa ttacctaaat cataagataa
     2281 atatcccaca gcacctccta caaatggaag attagtatca tttacaaccc tatacttatg
     2341 tagttcttct tttaaaactt ctaaaggatt ttcatcagta tctttatact ttaatacctt
     2401 aaatggttgg ctacttataa aagaatatct tcccaatttt tctttgtcca tagcactgtc
     2461 taatataaaa ctgtcatgtt catttctaaa aatagtaaat atctcaaaag aatttaattt
     2521 agtatttatc tctcttatca tattacatat gtaccctttc tctagcttct gttataaaat
     2581 ttttaagcat ttcatgacca tactctgaca aaatagcttc tggatgaaac tgtactcctt
     2641 ctattaaata tttcttatgc ctaatcccca ttatttcccc tttatcagta attgctgtaa
     2701 tttctaattc ttttggaact gtattggagt caactattag tgaatgatac ctagtgacat
     2761 ttaaaggatt tttaagtccc ctaaaaactc ctttattagt atggttgata ctatatactt
     2821 ttccatgcac aggctgttgt gcttttataa tatctcctcc aaaaacatgt ccaatagttt
     2881 ggtgacctaa acaaattcct agtataggtt ttttcccctt aaaatgctca acaatatcta
     2941 tgcaaatacc agcttctttt ggagaacacg gtcctggaga taacacaatt acctctggat
     3001 ttagttcttc aatgtcactt attttgattt cattatttct ttttactaca actgtttccc
     3061 ctaattcttc tatatattgg actaggttgt atacaaaaga atcataattg tctatcataa
     3121 gaatcatact gtaaatctcc cccctaaaaa ttattttatt aactcagaaa ctgttacaaa
     3181 tttaaaacct ttttcaatca tttttggaat aaacatatct aaagcttcaa ctgtatcatt
     3241 agttgcataa tcatgcaaaa gtattatatc tccattttgt actttattaa ttattttatc
     3301 tgcaatgcta ttagctcctg gatttttcca atctttcaca tctacacctg tccataaaac
     3361 aactttatag tcatgctttt ttgcaatttc tattaattct ttttctctat aacttccaaa
     3421 tggtggtcta aataatgtcg gtttttttcc agttacttct ttcaaaatgt cttcacactt
     3481 aacaatctcc tcttctatct gactagaact taaattactt atatcaggat gattgaaagt
     3541 atgatttcct atctcgtgac cttctttact tgctcttact aatggttctt tataccattt
     3601 tgcatgtttt ccagctataa aaaaagtagc ttttacattg tatttcttta atacatctaa
     3661 aacttgacta gtttctttag gatgaggacc atcatcaaag gtaattgcta ttagcttttt
     3721 ttcccttgaa ccatgtttaa tcaaaacatt tccattagta tccatcatca aagcagaaac
     3781 tttactaaaa ttagatatta acactattcc acaaaacaag actactgcta ttaagcactt
     3841 atatactgtc ttttttttcc atgatgtcat tttttaactt cctttcaaat attttagtta
     3901 tttataaaaa ttttgagtgt aatatgtttt atagcttcct ccaaatatta ctccaactcc
     3961 aatattatta tagttcccca aaatattttt tctatgtcct tcagagttca tccaagcttc
     4021 atgcgcatat attgcattaa tctgtcctgc tgctatgttt tctccagctg aagtatatac
     4081 tataccttct cttttcattc tgtcaaatgg agtttcattc ttcttattta catggtcaaa
     4141 aaaattatta tctctcatat cttcactatg ttttcttgaa ctcaaagttg cctgttcact
     4201 gtatctcaat gagtttaacc ctctttgatt tctaacacta tttacaaggt ctataacttc
     4261 aagttcaaaa cattttttaa gttcttcgct atcatctgga tataaatttt taaactcatc
     4321 ttcagtgctt ttatcaatta ttaaatatga acaaactcta ttttcttcat gaatatcgta
     4381 gaaaattgta atatacttac cttccttact tattatatca tattgatttt ctgagtttat
     4441 tatatatctg gtatttcctt ttctcttata ttttaatggt gtaatcttag ttctcactgt
     4501 ttgtctatct tgatttagtt ttatatcttg attttcacat gaattaatag aattactata
     4561 aagtgctact acttcatcat tttcaagtcc aaccattgca aattcttttc catattgatt
     4621 atatacatac caatcaaaac catattcact aatatcaatt ctaccaggtt tgccgatact
     4681 tgataaaaca tagcttaaat tatctcctaa gcttattttt tcaaaattct ttatatctga
     4741 ctctcctaag ttctcccctt cagtattatt tgattgtaaa ttttgacttt tttctttttt
     4801 atctttctta gaattatcat ttatatttat tttgtctaaa aattcattcc atatttcact
     4861 ttgaaaaaac ttttctttta aatcatatcc ttcttttaat aattcaggac tataaaataa
     4921 aacaataagt actactaaca ctaaagcaat taatctcttc aatatatctc tccttcttaa
     4981 aatcaatcaa aattttttaa tattgcattt attaagttaa tattgatagt gtatactaat
     5041 caatatcatt catacctatt tacagtaagt attagaaaaa agctttatct actaattaga
     5101 taaagctaca gtctcaagga aaatatttca tctaatgttc tatcaaatat agatggctta
     5161 tcaaatttcc aagtcccttc ataaaccaat tcattattta gataaaaagt tccttgccca
     5221 tgttttttac catgcataaa ttcacctata tactttttac ctttttccca tatataagtt
     5281 cccttaccat gaatcaaatt attcttccat tctccttcat aaagtacttc ccctttacta
     5341 ttatagcaaa taccgtaccc ttccatcttt ccacctttaa attcacctgc atatatacat
     5401 ccatcactcc acttataaat accttttcca tctttttttc cgtttttcca agttccaata
     5461 tattcatctt tactactata taacatattt ccaataccgc tcatcatatc ttcctcaaaa
     5521 taacctatat attcatttcc atttgaaaat ataaagtttc cttttccatt ttttttatct
     5581 tctttaaatt ctcctatgta ctccataact gttccttcat ctacaaaaaa agtatatgag
     5641 cctttcccct ccatctttcc ctctaagaat ttccctacat atacatcccc atttgaaaat
     5701 ttatagattt tattttttgg tttttttgta tcgacatcat ctatatcgcg tattgttttt
     5761 ccttccaatt ctaatacttc attttctgga gcaaaatcgc ccctttttaa aacacgtttc
     5821 ttcatgttct ttatacttct aatctctttg tttacttcat ttatatattt caaataatta
     5881 tcatacaaat taacatattc tttagataaa ttcataatgc cattcctcca acgttattcc
     5941 tagatatttt actaaatcta ttaaatttat tatatcctaa tagtggtcat gttttctaca
     6001 tattaagtaa ttttttcaat tttaatttta aaatgatatc ttttaaagtg atacacaaat
     6061 cattaataaa acctttattt tcgtatacta aatttaaata agctgagaga tataatatac
     6121 aaatttacat tataaactcc cagcttgtaa aattttctat aaatcttttg ctttaaaccc
     6181 tcttcctttt acttctgtaa catcatttac agatataaag gcctttttat catacttcaa
     6241 agctatatct tttatctttg gtatctcatt tgatgaaact attgtatata taatcttctt
     6301 tttttgatga gtgtatgcac cttctgcatc taaaaaagtt acacctctaa ctaaactctt
     6361 cattatatct tctgctatta aatcgacttc atttgacatt accataacag atttttggta
     6421 attcattgca tctttaataa aactcattga atacgcattt agaaatagac ctataagagt
     6481 gtataatgct aaatccaacc caaataaaaa acttcctgta agtactatca agccatttat
     6541 tgctaaagca gtgtctttca tttctatatt cttcttaatt tttagtattg cagctattat
     6601 atctgtacca ccttgagaag acctagttct aaatacaact ccgactccta aaccagataa
     6661 tataccacca tatacacttt gaagcaatat atcatgtata ggtataatat tacttatttc
     6721 ctgagttgct cctaataata atgaaaatac aatcatattt accaaacttg aaatacaaaa
     6781 ctctttttca aggtatatga accctaagat aaatattgga atattgatta aaaatgttaa
     6841 taaacctaca tttatatttg tcaaatagtt tatagatgtt gctatacctg tagcacctcc
     6901 actaagtaga tgtgctggtc ttaagaaccc atttattcct atagatgtca tcaacatccc
     6961 aaatagcatt gttgaatatc ttaaaactgg atgtttttgt agggtattct taacttcact
     7021 catttctttc tcccttcaat ctttctaaat ctttagaatt ttaaaagctt actcttacat
     7081 atattattcc tgatgtaata gataatattt caaataaatt ttttttataa tttcttgaac
     7141 tgtcatctat tacatcatct attatattat ctttttcata tatgtctagt gcaatctttt
     7201 atattctaat ctttaaaaac gttcaaatca tctcatttat gccttaaatg aaactttttc
     7261 tagcataata tgatactgct tattatttaa cttgcaatat agtaaattta tagtattttt
     7321 ttctaaatat ctattttcgc agtaatttat tttattttag caaattcaag tttagtaatt
     7381 gtctctttat aattaattcc taaatgaaat ttttcttttt ataatttcag tacatgattt
     7441 ttttatttca ttatttgcat ttttttcgtt cagtttttat tttaaatttt ttttatttta
     7501 ttttgtttta agtattcaag ttactcgtct taattttatt ttttatataa aattcattat
     7561 aattggaatt agtattactg taattgtacc agagactcca atagaaacac cactcatagc
     7621 tccttctatt tctccaagtt caaaagcctt tgtagttcct aaagcatgtg cagatgttcc
     7681 aagagcaatt cctttagcaa ctggatgatt tatattcccc agtttgaata ctgtagaagc
     7741 aaccatacca ccaaaaatac ctgtaaaaat tatggctaca actgttattg attctacacc
     7801 atttaaagaa tttgctaaag atattcccat tggtgttgtt atagattttg gaattagtga
     7861 atttattata cttacatctg aattagctat tttgccaatt atcaatacag atacaaaaga
     7921 aatcactata ccacaaccta ttcctacaat aatctcaaac atatgctttt taaataattc
     7981 aaattgttta taaagtggaa ctgcaagtac tactgtgaca ggacctaaaa agaaatttag
     8041 actatcagct cctatcttgt aattctcata agggatttta gatatgctta aaaacaatat
     8101 tattcctacg attgcaatta ataaagggtt acatatagga ttacttgttt ttcgttgaat
     8161 gtatcttcct aaattgaaaa aaactattgt tactattata ccaaatactg gtgtctgtaa
     8221 tacattatac atttttttgt ccttcttcct tcttttttat cattttctcc acaataattc
     8281 cagtagtata cattactatt agtgtactta aacatatgac taataaaagc actaatacat
     8341 tttcccttat taggtctaat gactttataa gtgatacacc tgctggaata aaaaatatag
     8401 ccatattatc gaggaaaaat ccacttacat tttctatact actcaagtct aaaactttaa
     8461 attgtagtag tagaaataat aatagcatac caactatact cccaggaata agtataattc
     8521 cttgtataaa ggaacttata tactctccta ttacccagat agataaaatt attaccaatt
     8581 gattgaaaac tttcattttc cacctccaaa aattaaagag attgcttcaa atcatatatt
     8641 tgtagtttta tcaacaagca aatatctttc tttaaaatag aaagttataa tttgacacaa
     8701 tctcttttta aatttatttt tattttaatt taataaaata catacttggt tgtttcaaaa
     8761 aacttaacta tataaaccta tttttcactt aaaatttctt ttatttttga attgttatac
     8821 atactgtctg gaacttcact tctcaaacta taaaaagtct gttgtgcatt ttttaaatcg
     8881 ccattatcat aataaagcat acccaactcg taatatgaat catcataata ctggtcattt
     8941 ttagtataag tatttatata ttttttgtaa tattttatag cttcatcctt atttcctaat
     9001 aatttatttg ttattgctaa ttgatatatt gactcagaaa tatatttttt agttttacca
     9061 gaagaaacta cacttttaaa attatctatg gctttttcat attctttgtt tttctttaaa
     9121 tttagagctt gataatataa atcttcctca tcagctacag ttatctctct ttctggttct
     9181 ttatttttaa cctcatttaa ttccttattc gttttttcta attttttact cgtttctcct
     9241 aactttttat cagtttcact taacttttcc tctttactac ttatttggtt attcaagttt
     9301 tctattctct ttttgctttg aatcaaatat atccccatac ctattaaaac acagccaact
     9361 ataatatatg gcaaaatact tttctttctt tttacttttt ttatttcttc ctttttactt
     9421 gtatcttctt tttcttcttc atttttttca aatacaggat tatctttatc tagctcttta
     9481 agtttgtcta aatatccttc tctctttttt acattaccta gtttatcata taacaaagat
     9541 aaaattacat atggctctat tagttcaggt tcttcttcaa ttatgttctc taaaatatgt
     9601 attgactcat aatttaattc ttcatttata aatcttatag aatgattgta tcttcctaaa
     9661 aaagttttaa aatcttcaga tgaaattaga tttacatatt ttctagatat atgattattt
     9721 tcataacata aagcagtata aaaactctca aatgatttag caaaatcaca ttttaataat
     9781 tttaaaagac ctctaagatt tagtatatca atgtctttag gatttatctc cagagcttta
     9841 ttaaccatat taatggcact agttatataa tttttatatg ctaaatccaa tgcattgtta
     9901 tatcttttat atgattctaa ttttaactcc ttagacgttg tatctgactt tgaatttaaa
     9961 gctccttgca agtttaatag agtttcttct ttataatttg tagtcacata actctgtttt
    10021 aatgctttta atgtttcatc accgtcttct tcttctatat taaaaaaagt caaatcactt
    10081 ttattatctt ctatcaaaga tatatctaaa atttttctag gcatgctttc tatgtcttgt
    10141 tttatatctt taactaaaat aatactcacc tccactctaa aatatatatt taagataaca
    10201 tatttaatat gtcaagtcaa aactatacat atttttccac cattctcata ataaaaaagg
    10261 taatcctctt cttttcataa aaaggttacc tctttttcaa tactattaac ttttaatgtt
    10321 gaattttttt acttttctat agagtgtagc tatacctata tttaatgcct cagctacttt
    10381 tttcatacct tctgtattac ttccatattt ctttatggca ttttctatag cttccctctc
    10441 aaggtcatct atagtcttaa cttcttgttt ataaagttca tctagtttaa aagatattaa
    10501 atcagacata ctccttacaa aagcagttaa ctccttatct tttgaaagta tcttttcttt
    10561 ttgttcttca ttaaacgcag ctatacccaa gattccaagt atatgctctc ccattttaac
    10621 gggagtacat atatcagcta actctttaca attttctcta tttgcacatt tattacagtt
    10681 ctcttctatt tctttattta taacatagga tttcccatct tttaaaacaa gagagtacac
    10741 tgacttctct gatattgtct ctcctatttt acctctgaat attcctgtac ctgctatcct
    10801 agttaaatct tttgtaacta aagtaacatc tacatccaag acattagaaa tattctcgca
    10861 aatactttga acgtaattcg ttatattatt taaattcaaa gtaatccctc ctatttagat
    10921 ttatattttt tattactcta cataaatcct attcttgaga actttaagct tgcggttgtg
    10981 gttgtgtctc attaggctta ttatcagttg gtggtgtttc tggtttttgt tgattatttg
    11041 ggttttgatt gtttgtattt tgattattct tattttcgtc tgtatttggt ttttcctctg
    11101 gttttggttc catagtccca actaatataa taccttcctt cttaggataa tatgaattag
    11161 ctattttttc agttttaatt tttttaccat ttgcatcatt aaatgttcta tatgttgata
    11221 cagaatatgc atttcttcca cttttgtcta ctttttcttt acctttaggc attgtagggt
    11281 cattaactct tttcattgta gtttgtgaaa cgccatctaa ttttgttgat attgttatat
    11341 tttgtttatc ttctgcactt ccatatatat tgcaaacaat ttgatttcct gaaacgtaat
    11401 tttttatgta aactggatgc tttagattat ttttaaataa gaaatctatt ccactatctg
    11461 caacagttgc atctcttcct tttggaacat aacttgatgg tatagtatga ttttttacat
    11521 taacaatttc taaacctgcg tatagcacag aattgtataa cgtagatgaa acttgacaaa
    11581 ctcctccacc ataatcttct tccatctcac cttccattat tacaggtgca cttttatatc
    11641 cattttcctt acttcttttc cctgtatgtt tattaaatga gaaagtttct cctggcataa
    11701 gtaatacatt acttatatta tctgttgcta attttatgtt tatacttcta tttatttgag
    11761 aagttttaaa tgttgttgaa tacgtaccta aatttgtatc tatattagca agttgctctt
    11821 tttttacttt tgctggagta gcttttacta ctaactcatt tttatagtta ccttttttag
    11881 tttgattatc aaaattctcc atactcttag aaatatccat tttaagacca tcagaatctg
    11941 gtataacctt gactttttca ccacttatat caagtgtagc atcttttaca tctacatcta
    12001 tttctttagc tatcttttcc atttctgctt ttaatttatt tttatcataa tttatcgcaa
    12061 ctactaagtt gctttttttg ccaaaatttg ctgaaatagt ttttgataga ttgccaaaga
    12121 aagcactctt tctatttaaa ttatatgcgt tttctactgt tttatccagg tcagtagata
    12181 aatttaaatc tttgctcttt actttccagc ttttatcatt gtaattaaaa tcaacattct
    12241 ctaatttgta ttttttagct aattcctgtt ttgcttgtga tttagttaat tttccaatat
    12301 ttactccatt aacatatgtg ttttttgcta ttttctcccc tttaaattgc atgcacatta
    12361 tgcctataaa aattattact actaatgcta cagctccaat agctataact agctttttgt
    12421 tcacattact catcccatac ttctccttac aataaaatta tttatatatc cctattatta
    12481 tattactaca taatgcgcct taaaacccca aaaacttttt aattatatat taatttaata
    12541 ttattaatta atattcattt agtccatttt ctgatatggc atatattttt tcattgtaaa
    12601 tattaatagc ataaacttct ttgcctctaa ataagccttt atttactaca gcataataat
    12661 aaatattttt atattttcca aacaattctt ctaaactctt tttacttttt acatatctat
    12721 ctgctttctc tatatctaat atctcaatct ttttatactt attttctttt tcatcaagag
    12781 tattagttaa cacaaactta gctttatctt ttgtaatacc aaattgataa cttccatctt
    12841 ctaatattct aaagtatcca tcaccataat ctactttgta atctttttta ccatctttat
    12901 atgaatataa atctttagtt tttgcactta ccaaaacttc atttattaat ttatcatact
    12961 catatacttc agcataatag tatttattat tttttatcat ttgtggaagt gaatttatag
    13021 atagatttgc cttgtctttg ccactttttt tagctattac atttataatg tcatcagatg
    13081 ttaaagtata caatgataaa ttctttttat attttttttc taaactctgt accttttcat
    13141 catctgaata atcgtctttt aaatactcaa tatattgtaa tgctctttca taatctccag
    13201 aagtatttgc ctcttcagct ctacttatat agtaatcata catttcttca atacattgtt
    13261 ctttcccatt ttgagccata ctgtaatact tttcatcttc ttttaatact ttattatact
    13321 tttcaatagc ttcataatac ttatatgctt tttggtcttt tactgctgag tcataaacat
    13381 ctctagactc tttgattgtt tctatattct gtttgtatac atctaggtta ctacccatac
    13441 catttaatat tgaaacagta cttatgtagt taatggcaat atcataagta gtattttctt
    13501 ctttatacat ttctctaact ctatcagctt gagctaataa gtcatccaca tctatactta
    13561 ttttgtttaa ttctttcacg gtatttatta atccaataaa actttcttga cttatgtctc
    13621 catctaaata tttatcacca ctatttaata gcaatttatt tattttttta gaaacagact
    13681 tattaaacct ttccattttt gaatcagaaa actttttttc tatacttgtg tagtaatcaa
    13741 cagctataga atactgtctt ttctctataa tctttaacaa ctcattagct tcatttccta
    13801 cactaaactc gttactactt aagaaaaata ctattatcaa agcaattaca cctaatgttg
    13861 ataatgaaat cgcaactata gatttccttt tttcatcaat aaaatctaca gccttctcaa
    13921 ataaatctct aaaattcatt atagttccct ctccatttaa taatctttat attttatggt
    13981 aaactaaatt tcttattggt tattatatca taattcttga aaaaaaataa aaacacaagt
    14041 taaatcgtgt ttttatttta cctatcaatc attattttat acataaatat ttcctttaca
    14101 taaattatga tatctttctt tttatatccc tagaatgact cttttgtttt ttattattac
    14161 tgacagtttt cgttcttgtc ttgtgtatag ctttgctatc aaatattttt ctagcattgc
    14221 ataaaacaat tccaattgtt aaacaaactg taagcacact tcccatcaaa aagtaataat
    14281 ccaatcctat atgtactccc ataactgaat ttattaacac taatgcaaaa gtcattgcaa
    14341 aacatatcat catggagata actaaaattc ttagaatttt tttcaatcca ctcacctcgt
    14401 ataatcacta aaattgacac taccccttat ccattactat accaaattag taaaaaaata
    14461 gcaattacta ttgtcattta tattaggaaa atattaaatc tttttaataa atatcttttt
    14521 ttgacataag gattacttaa cttttaataa aatagtaaca ctaatatgta ttaattaata
    14581 gaataaaata aatgataatt tattgagggg tgatttacat gccaaatttg ccaagtttag
    14641 ggtcaaaggc tcctgatttt aaagccaata caacaaatgg tcctattaga ctctctgact
    14701 ataagggtaa ttggattgtt ttattttcac atcctggtga ttttacacca gtttgtacta
    14761 cagaattttt atgttttgct aaatattatg acgaatttaa aaaaagaaat acagaactaa
    14821 ttggtctaag tgttgatagt aacagttcac atttagcttg gatgtataat atttctttac
    14881 ttacaggtgt agaaattcca tttcctatta tagaagatag agatatgaga attgccaagt
    14941 tatacggcat gatatcaaaa ccaatgagtg atacatcaac tgttcgctct gtatttatta
    15001 tagataataa tcaaattcta agaacgattc tttattatcc actaactaca ggaagaaata
    15061 ttccagaaat acttagaata gtagatgcac ttcagactag tgatagagat aatatagtta
    15121 ctcctgcaaa ctggtttcct ggaatgccag tgattttacc ttatcctaaa aactataagg
    15181 aattaaaaaa tagagttaac agttgtaata agaaatattc atgtatggac tggtacttat
    15241 gttttgtacc agataattat aatgatgaag aagtgagcaa gaaaattgat aatacttgta
    15301 gctggaaaaa agaacatact aaaaacattg aaaatgaatg taactgtgaa catgaacatc
    15361 atgactacct gaacaaagct cttgactgta aacaagaaca caagactgat attaaagatg
    15421 attgcaatca tgagaaaaaa catactaaaa atactaacaa agttcacaac tccaaacaag
    15481 ataagtttaa agataagtct tgtgatgaaa tgaattttaa ctatgataaa gatgaatctt
    15541 gtgacaaaat aaattctagc tataacaaag aagatagtag ttatgaagat ttctataaac
    15601 ataattataa aaactacgat tatactagcg aaaaaaatac taaaaaaata gctatgaaaa
    15661 ctttaaaaga ttctaaaaaa ttagttagac cacaaataac agacccatac aatccaatag
    15721 ttgaaaatgc aaactgtcca gatataaatc caattgtagc agaatatgtt cttggaaatc
    15781 caactaatgt agatgctcaa ctattagatg cagttatatt tgcttttgct gagatagacc
    15841 agtctggaaa tttgtttatt ccttatccta gatttttaaa tcaattactt gctcttaaag
    15901 gtgaaaaacc tagcttaaaa gtaattgtag ctattggagg ttggggagct gaaggattct
    15961 ctgatgcagc attaacacct acatctagat ataattttgc aagacaggtc aatcagatga
    16021 taaatgaata tgctttagat ggaatagata tagactggga atatcctgga agtagtgcat
    16081 ctggaataac atcaagacct caagatagag aaaactttac actcttacta actgccataa
    16141 gagatgttat aggagatgat aaatggctta gtgtagctgg aacaggagat agaggatata
    16201 taaattcaag tgctgaaata gataaaatag ctcctataat agattatttt aatcttatga
    16261 gttatgattt tacagcaggt gaaacaggcc caaatggtag aaaacatcaa gcaaatcttt
    16321 ttgattcaga cttatctttg ccaggatata gtgttgatgc aatggtaaga aatcttgaga
    16381 atgctggaat gccttctgaa aaaatccttc ttggtatacc attttatgga agattaggtg
    16441 ctactataac aagaacttat gatgagctta gaagggatta tataaataaa aatggatatg
    16501 aatatagatt tgataatact gctcaagttc cgtatttagt taaggatgga gattttgcaa
    16561 tgtcatatga tgatgcttta tcaatattct taaaaactca atatgttctt agaaattgtc
    16621 taggtggtgt attctcatgg acatcaactt atgaccaagc aaatatactg gctagaacca
    16681 tgtctattgg tataaatgac cctgaagtat taaaagaaga acttgaaggt atttatgggc
    16741 aattctaata gaatgtagta aaattttata gttatataaa aagcagattt tattatatta
    16801 aataagttgt atctaattaa tactattatt aattgtttat aaattagata cttatttttt
    16861 cataatacaa tctgcttttt taatcaaaat ttatttaatt acaagttgtt taattataaa
    16921 gtaggaattt agatatactc ttataccatg taattatgta taaatatttt tttattatac
    16981 accaatagct tttttagcta atttatcagc ttcctcatta tacttatttc cagaatgagc
    17041 tttaaccttt gtaaatttta cattaagctt attcttaata ctgtcataga aatttttata
    17101 tgctatagtt ccttctttat ttgttttcca aactccagta caccactttt caatcccttc
    17161 atagtcaaag tataaaatca agttttgaac attattgtca atacagtact gcattgcaat
    17221 tttagaagcc tctatctctc cagctacatt tctcatactt actaaagttt tctcttttcc
    17281 tttttcacta taagttttta taacttcatt attttttaaa atcacaacac ccgaaccata
    17341 catttttact gaatgttcat aacttccatc tacatatgct tctataaact ccttatcttc
    17401 ttgaaaactc aatttttctg acccatcaat atattcttta gcttcttgaa aagtagaaaa
    17461 actcttatat tcagctcctg agaatccatt tacttgtttt ttgcactcat cccatgtatt
    17521 atatacacct atatttttac cttttttcac agcataaaac ttttgttttg ccaaatatat
    17581 cactcccact cttaattttt atcttcttct ctcataaata tatctaaatt tataagatag
    17641 tttctatcaa aatattttac tttcatatct ttttttataa tatcaatatg gtacacttct
    17701 ccttttacag ctctttgtct tatattttca tttatatctt tttttataat atccttatca
    17761 acatcattgt tatccatttt agcatcaaca ttcaacgctt ttatagtatc aaatataaca
    17821 gattttagta ttaaacttct agatttattt aaatgtcgcc ttatataagt ataaaactca
    17881 ttgctattaa cctttgaaat tttacaatcg tgtaaatctt tctctaatct ctttaacaca
    17941 cttatattag ttcctacatc aaaaatcaca aaacatccat agtcatattt gcttcttaaa
    18001 attctcccca tagcttgctt tacctttatg gtcatttgag gataatttat agtatagtag
    18061 tctataccat acttcttcat tatagtaaaa tacaaagggt ctttaggatt tagattcgga
    18121 agcttgtcta aagttacaca aaccaaacca tcccctggaa tgtctacgcc ttcaaaacat
    18181 ccttttgagc caagtactac acattttcta tttaaatcgt taaggtgtct aatgcccttt
    18241 ttatcagcat aaatttctaa gttaaaacta tgtaagtatt ttttcaatat ttcataagtt
    18301 ttttcttgtc tatctttact attaaaaaga gctaatgtat gaccttctgt aatctttgat
    18361 atattagata taattttact catctcattt ggaaattcag aatttctgta ctcacaaata
    18421 tcagaaaatc caactacaga gactctattt ttataatcaa aaattggttc tataatcttt
    18481 tccacattct taactctgtc tattcctaat gtatttttaa aataagacat actttcagat
    18541 aaactcaaag ttgctgataa aaatattcct ttttctaatt gagataaaat attttcttca
    18601 aataaatctg ctatttttaa tggtactact cgaaattcaa agtcattata attcttatca
    18661 atttcaacta tccttgcata atcatccttt tcatcatact ctaaaaagat ttcaaatata
    18721 atctttatat cctctaaatc tcttgtcttt gcctttccaa atttatatat atctgcttct
    18781 ttatctatac tatcatcatc aatattccta taaattatta ttattattga aactaaattt
    18841 tttattattt tctcacaact caactttatc ttttcactat aggctctata acttatttca
    18901 gtatctatac catctttttt tagttttccc acaatttcat ctatttgcaa attcagttcc
    18961 catcttaaat tataattact tacattatta tattcattac aattaccaaa ctttaatatt
    19021 gaatctatct cttcaataat gaaatttata cttctagcaa ttttttgttt atcttctctt
    19081 ccaattttta atacattata aaatttatca aaagccttta tttttctcat atttctacta
    19141 tattttttat atataaagct actattttga ataaactcgt aaggataaat ttcttgtaat
    19201 aagtacctta aagactttga atttataata cttgaaaaga aatcatatcc tttttcagtc
    19261 aaattatgtg cttcatctac tattatattt tctaaaggtt tttcatcctt atacggccat
    19321 ttagctaata gtgaatgatt tactactgtt atatgttctt cttttaattc ttctattctg
    19381 tttttatata aacaatcttt tttgcatttt ttaggtctgc acatatttgg gtcacaactt
    19441 acatttctta agtgagtact tatttccttg aagttgtcaa acactaaata atttatttct
    19501 tctatatctc catacttgcc accttcaact aatctcttca aaaatattaa agataaaatt
    19561 tctcctttag tagggttttg agactcataa tcatctatat atgcttccag tctatctata
    19621 caaatatagt tattttttcc ctttatatat ccgtaactta cttttccatt tagacccaaa
    19681 gaatctaaca catttggaat atctttattt attaattgta tctgcaattc ctttgtgtca
    19741 gtagaaatta ataatctttt tttattaatt ctagcctcta atattgcagg caataaatat
    19801 cctacacttt tacctatacc agttggtgcc tctatacatg caattttttc ttcatcttcg
    19861 ctatttctaa acaactctct tattgtttta gtaagctcat attgaccagg tctatattca
    19921 tatgtaaatc cttctttatt ctcccaaata gttttgtatt ttaataattc ttcataattt
    19981 ttttcttctt catttaaaat ttttaaggtt tctctttcct tcttcttttc ttcttttgag
    20041 tcaaatatat tatattcttt ttttatattt atattatttg ataaatcata atttgcctct
    20101 tctaaaaatt tgctccattc ccatgttgga agattaaatt ttttaagata tgaatttatt
    20161 ttaaaactca atggctccaa agtagttttt tcatagttat taaattttac taaaaggcaa
    20221 tttacaatat ttattatatc aatagcatct gataaagctc tattttccac tttagaatta
    20281 caatttgtta aagtattttt taagtattct aagctataat ctttatgata tggttctaaa
    20341 ataactgcta attcaattaa atcaataaat ttatttttta actttggcat atgatactca
    20401 aaaaactttt ttttcagact taaatcatga catataatga aactatcttc tacaaaattt
    20461 acaagtctat cttccaccag ttctatttta ttagctattt ctaaatcatt tttatctaaa
    20521 tttttacata ctgagaatac ttctataggt acctcaaatt tgttttttat taatgattca
    20581 taggtatata ttttccagtc tttaaccttg acagctccaa cttctaaaat ttctgaattt
    20641 aaacaatcta atccactaac ttctatatct aaaaaaacaa catcattcaa tacacttatt
    20701 atattatcca tttttatatc ccttcaagtt aaaccagcct aacttattta caatcatttc
    20761 tctttatatt ttatcattta tatacacttt atttaagata attaatatca actttatatc
    20821 ttacagtaaa cttctttcat ttattatttg tatatgtgaa tatttttaac tataaatatt
    20881 caaactatat tataagaaat atactttaaa ttttaaataa gaggtgaaat tgtgaaaaaa
    20941 gatagcctaa aaaaatacat tatgatagga gcatttgctc taacactttt tggaattgct
    21001 agtataaatt ttaaatcatt agataaaaca aaaactcaaa tttctagtcc aacactcgac
    21061 acacatgaat atgattggta ttttaaccct agggaagatg gtaaacagcc atcacctata
    21121 aaagaagctg atttttttaa aaaatacgga gcttattatg taggaaatcc caatgaaaaa
    21181 gttatatatc tatcatttga tgctggatat gaaagtggaa acactccaaa attattagat
    21241 acgcttaaaa aacacaatgc taaagctcaa tttttcgtag ttgaaagtta tataaaaagc
    21301 aatccagagt taataaagcg tatggaaaaa gaaggacatt tagtttgtaa tcattcaaaa
    21361 agccatccat caatggcagg tattacagat tttgaaaaat tcaaagaaga aattacaagt
    21421 gtagaaaaag catacaaaga tgttactgga aaagaaatgc ctaagtattt tagacctcca
    21481 atgggtaaat ttagcgaaca atctttaaaa tatacacaag atttaggata taaatcaata
    21541 ttttggagtt ttgcttatgt agattggtat gaaaagaaac aacctactca tgaatttgcc
    21601 aaaaacaaaa tttattcaag aactcatcct ggtgcaatag ttttactgca tccaaattca
    21661 tcaacaaata ctgagatatt agatgaggtt cttacacatt gggaaaaaga aggttataaa
    21721 cttaaaacac ttgattattt aaataataaa aaatagtatc ataaatttaa aaaaactcta
    21781 tcatgaatta tagtaaaaat atagttattt ataaatactt taattctcga tagagttttt
    21841 tattttttat agctatttaa aaagaattgt ccattcattt atctctttgt atcctatatt
    21901 atgatatatt ttacctgcta catcattatt ataaaaaaga catggaactt ttccttctaa
    21961 caacaattct ttactcaaat tgtaaaccat ataacttgca agtccttttc ccctatatct
    22021 tttatctgta cttacagaga ccaccattgc caaaaaacta gtttccatac cagttgacac
    22081 agtgctaatc atagtatcat catttctaat aaaatatgct cttacatttc cctcttttaa
    22141 atgaactttt ctttcttcta tgtaattttt acttacttta tagtctgacc tatttaaaag
    22201 ttttcctatt tcatcaatat ctttttcctg agcattttct attttatact ccttcatgtt
    22261 ggaaaaatct atttctttta tttcttttaa tacacaaaat ttattatcta gtttttcata
    22321 aaaatctttg tatttactta ttagtctatc tattacacat ttttttccac ttatgctttc
    22381 tacatctaaa tcttttatat gattcttcat ttctcctata ttaaaattat ttttaaaact
    22441 ataaattata agtgttgact tattttttaa tattatagag gttatatttc caccttctac
    22501 ctgagaccat atatcttgat attgagagct aaatccataa ttctcaacgt atccaattat
    22561 gaataaattt aaactttctt catgtgatac atattgccaa aattcttttt ctaaagtatt
    22621 atctattttt acaatcaagc agatatcctc cttctatgtt aataatgata taccttataa
    22681 tttatagtat catttaaaat cattatattc taccaaatat tctaaatttt taaatatcta
    22741 tgcaatatgt tcgtatttat ttttatattc tgttactgct tcactccaag cttcttctac
    22801 tgaaatacct ctcttatttg caattttttg tgctaattct attattttca tagcttttct
    22861 tgatatattc atatatattc ctcctctaaa ttatcataag ttattttaaa gttaaatttc
    22921 tatagtcatg ttatatattt atttacttat atcttactaa ttatttatgt actaaatatg
    22981 aactacaata gaagaaattt agaacttaat aaaacataaa atttatatac agttcatata
    23041 tatttgtcat atcacaagac aagtgatata atttattatc attttactgg gggtgatatg
    23101 ctatgaaaat aagagctgaa tatacatgtc ctcttgaaat tgtacatgac ataatcaaag
    23161 gaaaatggaa aactatcctt atatttgaat taagacaatc taataaaaca ttttctgaat
    23221 tgcaacataa tatttatgga ataaatcaaa aaatgttgat tcaacaatta aaagagttac
    23281 gtgattttgg gattattgat aaaataagtt ctgctggata tccattacat gttgaatatt
    23341 ttcttactaa tcgtggtaaa aaaatgttaa aagcagtgga aataatgcaa gaaataggaa
    23401 tcgaatatat gcttgaacat ggtcaacaag aaattctaga taataaagga atttgctaca
    23461 ataagtaatc ctatacatac caaaaagtaa gtaatttaca ttttttacaa tagtattata
    23521 ataaaaataa ataggaggta taatcatgtc tatagcttta atattgattg atattcaaaa
    23581 tgattatttt aaaaatggaa aatgtgaatt atttcaatca gaagaaactg ctgaaaatgc
    23641 taaaaaaata ctactgtttt ttagaaaaaa caatttacca gttattcata tacagcatat
    23701 atcaacagat aaaacagctt ctttttttct tcctaatact catggttcag aaataaataa
    23761 aattgttttt ccactagata atgaagaaat tataattaaa cacacaccag atagtttctt
    23821 tgaaacagat ttgcaaagta tccttgagaa aaaaaatatt actaatctag taatctgtgg
    23881 aatgatgagc catatgtgta ttgatacaag tgtaagaaca gcaaagaaac ttcgatacga
    23941 cataactcta ataagcgatg catgtacaac gaagaattta tcttggaata atatagaaat
    24001 taatgcaaat actgtacatc aagtattcat ggcatcacta cagaacagtt ttgcagatgt
    24061 aaaaaacaca gataaatttt tatctaacta ttacaaataa aaacatacac tatgttaaaa
    24121 gagcaagttt gatttattta agcttgctct ttaatcattt aaaattagat gctttctatc
    24181 atttttatat acataatata cgtttataca ttttatattc atatataact attatataaa
    24241 ttttaaactt tactatattc acataaaaaa tgtagtatta tagtatatat aatattatat
    24301 agaagggggt gctagataaa tgaaccatca tatattgatt caatttgaat atatagcaag
    24361 aatcattact gctggtttct gtggggcttt aattggatat gagagaaaga atagactaaa
    24421 agaagctgga atacgcactc attttatagt ggcattagct tcagcactta taatcattgt
    24481 ttccaagtat ggatttaatg atatagtggg tactccaggt attggtctag acccagcccg
    24541 cgttgcagca caagttgtca gtggtatagg ttttttaggt gctggattaa tatttattag
    24601 aaatcaatca gtaagtggtc ttactacagc tgctggtata tgggcaacag ccggtacagg
    24661 tcttgccatt ggctctggat tatacttagt tggtattgtt tcaagtatca ttattgttat
    24721 tgtacagata acacttcata gagataggaa atggatgaaa ctccctacag cagagcaagt
    24781 ttctcttaaa atttctaaca gcactgattc catagaatat attgaaggta aatttaaaaa
    24841 atctaatatt gaaattataa acatgaatat taaacgcctt gatggggatt ggttagaagt
    24901 agttatatat actaagttac ctaaaggata taaaaaaaca aatttattaa aattatttag
    24961 cgataatcct cttatagaaa ctttagaaat ataatataaa aattgaagct taaaaataat
    25021 aatatattta atcaaggtca atatataaat aattaaggga tgagtctata taaaattcat
    25081 cccttgtttt ttgccattca tatatatttt ttcaagatat ttttgtttat ctattatcta
    25141 gccaagtaac ccttgtgaac taaacttttt aatacaaaaa cttcaataca aaactaaata
    25201 taatttttat taaacattct atacatatag tttgtttctt gagtgaaaaa tagatttcat
    25261 attgttaaga tattgttaaa tatttcaatt ttctgacaag tatgttgaac aataacttat
    25321 cctagtgtat tattttagtt gtagaataaa actaaatcgg caaaactaga gaaatttagt
    25381 gacgcaaagc tatagggact aacgcttata aaataaacta tgagttatgt cagccagttg
    25441 ccaaaaagat attgttcttt ttgttttatg aaagtttttt agggggatag taatatatat
    25501 gtttagagat gaaatggata aatgtacaca catgttaact gcttatatta gtagtttata
    25561 tgattattgt gattttatag atacacagct agatgatttt atactagagt acggagaaaa
    25621 tgtagtagaa tcttgtttac atcaagtgat ggtattggta agtaagtata attaaataga
    25681 aattgttttc tagagaaccc tttttagttt tatctacatt taaaaatgtt attatattaa
    25741 aaaactaatc ttccatatat gcaaagtttt cttccatata acaatttctt cccatttata
    25801 ctttttcact tgtatcttta ataaaattca acttcaaaaa ttattctatt tcttatcttt
    25861 acaccaattc ctatcagtat tatcctttat ataggttcta actatttcgc cacaatctaa
    25921 acataaatct gaatatatag tagattgtac tggaaaagtt ttatttataa ctttatctaa
    25981 ttttccatct cctctatatt ttaatccaat agtttcagca tatatgccac cagttgttaa
    26041 aattcccttt tcaatattta cacttccaca attagaacat ttcacattta taacccccta
    26101 aacaattatt ttttcatgct atcttataat ttaaaatata atttgctacc gattttataa
    26161 cacagtggca aatcggtgcc aaatcaacat tttttcattc tatttaccat ctaatccatt
    26221 gaaatataga tatttagtta taggaacttg aatttctgtt acaaactcct ctacaattcc
    26281 agtctcatgt atatctatct tatagatttc tatagggtca gcaattatct tataattatt
    26341 ttcttctata aatttaaaca ccttctccat atacatctta ttattagaat tactcccagt
    26401 ataatttaaa gttacataat atccttcatc aaaaactata ttatatactt tctcattact
    26461 ttccagaaaa caaaatacag atttaaactc attaaatatg cctttattaa ttgcttcagt
    26521 agaaaaggca gaaccaatat tgttgtttcc aagtatatta aatctatctt catattcttt
    26581 ctgaagtttt tgaattaaaa aatcgaagtc ttcatccctc tttatatcag catttaattc
    26641 taaagctttt cttttttcta tatatacaac ctctatctca tctatttttg tattttgaat
    26701 tacactttca attgaactca atctttttat aatattttcc ttatggctta taaattcagc
    26761 tattttttta tcaattaaat cgagttcttc atttaaaata tttttcgtag actctatact
    26821 tctgtcatct aggtattctt ttattttctt cattggaaag ttcaagcttc taaattcttt
    26881 tattaaattt aatttccata tatctgatat attatacatt ctatatccat ttatatctct
    26941 aactggtctt agaatgccta tttcttcata atacattaat gaatctctac ctattccata
    27001 tattttagat atctctccta ttttatagta atctttcatt tatataataa actcctccga
    27061 aatcttattt ttattttaaa tcaacattat tactctataa tactatagta ttagaatata
    27121 ctaaaaaaat ctttaaaaca aggataggaa aataaacatc ctaataatta attattaaaa
    27181 tatttttata ttgtaatatt aggtatagta tttataactt caactttttt ataaatatat
    27241 tttagtttta tatagtttta tgtttacagc tattatatcc atctctatgt aatatttttc
    27301 atattaaaac tgatttctat agattaatag tttataaatt tagtaaatga atatttatta
    27361 tagtctttaa caaaaaatac aagaataaac ttaattttaa taactaaaat taattatgca
    27421 aaattattaa tatggtatta aattatgtat aaaaagttaa ttatgaataa aataattatg
    27481 cctttgaaaa atcattaaac ttaaaattaa ttataaaaaa actctacctt actaactata
    27541 cagctcacaa tctatctatt tatgaaaaaa tatgatataa ttgcattcaa aaaggaaatc
    27601 tttcaatata ctagaattta agcattaact aattttatct aatattatta ctaaactaaa
    27661 aaatatttgt atttgtagca ttggagc
//
LOCUS       DAYAPA010000046        27680 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.046, whole genome shotgun sequence.
ACCESSION   DAYAPA010000046 DAYAPA010000000
VERSION     DAYAPA010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27680
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.046"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            81..1685
                     /gene="rho"
                     /locus_tag="ACGI59_001805"
     CDS             81..1685
                     /gene="rho"
                     /locus_tag="ACGI59_001805"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFG9739591.1"
                     /translation="MRRIFLILDDKVLEGLGSKTLVELREIAKELKIKSITTYKKNEL
                     IEIINSKSKNEEKVNEETKEKNEDIKEDIKNRKENIERESIEKEVEYKSPTKSYNKKE
                     IDTQNISQNQEHRNQRNDYNKTTSVNDSNKSSNISNRMVRNNNKNYYMPKQVDESKIV
                     DEFNTSKEDEVVGVLEILPDGFGFLRGSNYLSTEGDVYVSPSQIRRFNMKTGDKIKGI
                     TRHPKSGEKFRALLYVQKINDENPDTAIQRNAFETLTPIFPEERLTLETNRNEIATRI
                     IDLISPIGKGQRGLIVAPPKAGKTVLLKSVANSIAKNHPNVELIVLLIDERPEEVTDM
                     KESIEGDVIYSTFDQVSSHHVKVAEMVLNRAQRLVEHGKDVVILLDSITRLARAYNLT
                     ISPTGRTLSGGIDPGALHGPKKFFGAARNIRQGGSLTILGTALVETGSRMDDVIFEEF
                     KGTGNMELHLDRKLAEKRIFPAIDIYKSGTRRDDLLLDDEEKTALWRLRREMSNNSVM
                     EITDKVIELIKRTKDNKEFVKSIKNL"
     gene            1838..2038
                     /gene="rpmE"
                     /locus_tag="ACGI59_001806"
     CDS             1838..2038
                     /gene="rpmE"
                     /locus_tag="ACGI59_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018591942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HFG9739592.1"
                     /translation="MQKEIQPKYNPVEVRCACGNTFVAGSTKDEIKVEICSECHPFYT
                     GKQKNIEKGGRIDKFKKRFKMD"
     gene            2183..3076
                     /locus_tag="ACGI59_001807"
     CDS             2183..3076
                     /locus_tag="ACGI59_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HFG9739593.1"
                     /translation="MAKQAVGGQAVIEGVMMQSKDKRAVAVRKSDGEIALKEDRIKSW
                     VRDKNIDKIPFVRGSFVMIDTMIQGIKSLNFSSEFFMEEAEEDKFDLFIKKIFKDKAN
                     DIIIIFSLVIAMLLSAGLFIFIPTLVGGAFSKVMPNDFMLNLIEGIIRIAILFAYIVL
                     ISRSKDIERVFQYHGAEHKSIYCYENDLELTVENARKFKRLHPRCGTNFLFIVMAVSI
                     ILFAFFGWPNPILRIFMRIICVPIVAGLSYEVIRVLGKYDNGFTKIIAYPGMMLQYFT
                     TKEPDDEQLEVALEALKAVVD"
     gene            3094..3942
                     /gene="prmC"
                     /locus_tag="ACGI59_001808"
     CDS             3094..3942
                     /gene="prmC"
                     /locus_tag="ACGI59_001808"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFG9739594.1"
                     /translation="MTIKDIIIKYSDKLKDISDTPRLDTELLLQKTLGVDRLYIHLNL
                     NKELTEEQKTKFMGFAEERLNGRPIAYIVENREFMGLDFFVKEGVLIPRPDTETLVEE
                     IIEICREKKDVSILDIGTGSGAITISLAKYIENSKIMSFDISETALEIAKKNAIINEV
                     GEKIKYINSDLFTAISDSNIKFDIIVSNPPYIKKQDIETLHKQVKDYEPYNALEGGED
                     GLDFYRRITEQGKKYLNKCGILAYEVGHNQAEDVINIMKSNGYKKIYTKKDIQGIDRV
                     VIGYNI"
     gene            3986..5050
                     /gene="prfA"
                     /locus_tag="ACGI59_001809"
     CDS             3986..5050
                     /gene="prfA"
                     /locus_tag="ACGI59_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFG9739595.1"
                     /translation="MLKKLEVLEDTYKDLSEKIGDPDVINDQKVWQKYIKEHADLEPI
                     VMKYREYKSVLDSIKESKEILQEESDEELRELAKMELAEMEEKVAPLEEEIKILLLPK
                     DPNDDKNVIVEIRGGAGGDEAALFAGDLFRMYSRYAERRRWKIELLSASDTGVGGYKE
                     VSFMIKGKGAYSRLKYESGVHRVQRIPSTESGGRIHTSTSTVAVLPEVEDVEVEINPN
                     DLRIDVFRSSGNGGQSVNTTDSAVRVTHIPTGEVVSCQDGKSQLKNKEQALKILKARL
                     YDKALAEQHKDIAAERKSQVGTGDRSERIRTYNFPQGRISDHRINLTLYKLDAFLDGD
                     IDEMIDALITVDQTEKMTAI"
     gene            5179..5859
                     /locus_tag="ACGI59_001810"
     CDS             5179..5859
                     /locus_tag="ACGI59_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HFG9739596.1"
                     /translation="MILKVTIIGLLAGVIGTGLGGVISAIFKREVDKYLSFFMGLSGG
                     IMLAVVVFDLMKESMDKMGIINTVIFTFVGALITMYIKTKLDVSGNMASGYLIFISIL
                     LHNLPEGLAIGSSFMSTESLGITLAIVIGLHNIPEGLAMALGLVCNKMKLSKVILFTV
                     IAGLPMGLGSFLGVYFGGVFTSLIGVFLATAGGTMMYVVLEEIFPHSKSVYCIIGFLL
                     GTMIVNYI"
     gene            5888..6928
                     /locus_tag="ACGI59_001811"
     CDS             5888..6928
                     /locus_tag="ACGI59_001811"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFG9739597.1"
                     /translation="MKTIISNIDINNIDYEEIKIQAKLLREGKTVIFPTETVYGLGAN
                     ALDENAVKKIYEAKGRPSDNPLIVHIYEKEEVYDLAKDISDKAKLVIEKLWPGPITII
                     LNKKDIIPYKTSGGLDTVAIRMPSNVIARAIIKEAGIPIAAPSANISGRPSPTKAKHV
                     YEEMNGRVDGIVLGGDSNFGLESTVLDLTEETPMILRPGSITKEVLEELLGEVKLDPS
                     LSKKEDNQKAKAPGMKYKHYSPNADVYIISGKRENVATKINDMIKSNRDKGLKTGVMC
                     ISRNREFYDGEVIDLGNSLEEVASNLFDALIEMDKKGVDVIYSEEFPKTGVGQAIMNR
                     LLKSAGYKVIKA"
     gene            6945..7394
                     /locus_tag="ACGI59_001812"
     CDS             6945..7394
                     /locus_tag="ACGI59_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight protein arginine
                     phosphatase"
                     /protein_id="HFG9739598.1"
                     /translation="MNILIVCTGNTCRSPMAEAILRKAIKESGRSIEEYSISSAGIST
                     ANGMGASENSIEVLKEIGIDLSNHRSKVITKKLIDESDIILTMTKSHKEILVQAVPKC
                     KEKVYTFKGFANKNEEDISDPFGGNLDIYRSTMREIMYSVNEIVKKI"
     gene            7411..7863
                     /gene="rpiB"
                     /locus_tag="ACGI59_001813"
     CDS             7411..7863
                     /gene="rpiB"
                     /locus_tag="ACGI59_001813"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HFG9739599.1"
                     /translation="MKIGLGCDHGGYNLKKEIISYLESKGIECVDYGTNNATDSVDYP
                     VYGEIVANSVINKEVDYGILCCGTGIGISLAANKVPGIRCAVVSDVFSAKMSKAHNDA
                     NMLSLGERVLGKGLALEIVEAWINTDFEGDRHARRVNMIKSIEEKHNK"
     gene            7899..8528
                     /gene="upp"
                     /locus_tag="ACGI59_001814"
     CDS             7899..8528
                     /gene="upp"
                     /locus_tag="ACGI59_001814"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011971477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFG9739600.1"
                     /translation="MSKVVETNHPLIQHKLTLMRDKNTGSKDFRELLTEIAMLMGYEI
                     TKDIPLKDVEIETPIQKTSSKVVAGKKLAIIPILRAGLGMVDGLVSLMPAAKVGHVGL
                     YRDPETLKPVEYYCKLPQDIGERDIIVVDPMLATGGSAVAAIDLLKSKGAKSIKLANL
                     VAAPEGIAEVQKYHDDVDIYVASVDERLNEHGYIIPGLGDAGDRLFGTK"
     gene            8823..9638
                     /gene="yqeB"
                     /locus_tag="ACGI59_001815"
     CDS             8823..9638
                     /gene="yqeB"
                     /locus_tag="ACGI59_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent molybdenum cofactor
                     biosynthesis protein YqeB"
                     /protein_id="HFG9739601.1"
                     /translation="MKNCIVVRGAGDLATAVIHKLHNSGFKVVALEIEEPLAIRRQIS
                     FCEAVYEKKVTVEGVECIFCNTEKEIEEVLSQHKVALIIDPKGEKLSKLNPIVLVDGI
                     LAKKNLGTTKDMAPLTIALGPGFCAGEDVDVVIETMRGHNLGRIITNGRAEKNTGVPG
                     AIKGFSKERVMHSQYDGIMENVCKIGDIVEKGDLLCYINNYEKKHEVRATISGVLRGL
                     LKDGAKVNKNLKILDIDPRKEEVKNAFTISDKARCIAGGVLEAILNNGIYPQN"
     gene            9864..10301
                     /locus_tag="ACGI59_001816"
     CDS             9864..10301
                     /locus_tag="ACGI59_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HFG9739602.1"
                     /translation="MRPSWDEYFMEIAEVVKKRSTCIRRQVGAVIVRDKQILTTGYNG
                     SPRNLEHCENIGCKRQELNIPSGERHELCRALHAEQNAIIQAAHNGISVDGATLYVTT
                     RPCVLCAKMCINAGILKIVYEGDYPDDMSTELLKEAGIELIKF"
     gene            10486..10713
                     /locus_tag="ACGI59_001817"
     CDS             10486..10713
                     /locus_tag="ACGI59_001817"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HFG9739603.1"
                     /translation="MSKRNTHTEIAKLFSLISQIGLMIVISILGCTFLGKFLDSKLNT
                     TPVLTIIFLLLGVGGAFSGVYKTLIVYTRRK"
     gene            10718..11104
                     /locus_tag="ACGI59_001818"
     CDS             10718..11104
                     /locus_tag="ACGI59_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HFG9739604.1"
                     /translation="MDIKLLNQVRSVTKGIVIFDVVLIVLMLITSTLSKPVLLGLIFG
                     SIIAMLNFRLLAISLEKSVTFPAGKAQAYASAQYAVRLFIMAVVLIASATVPHLNIIG
                     TALGLLSTKFVILSKNFINKLKRKEA"
     gene            11107..11811
                     /gene="atpB"
                     /locus_tag="ACGI59_001819"
     CDS             11107..11811
                     /gene="atpB"
                     /locus_tag="ACGI59_001819"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFG9739605.1"
                     /translation="MNQAHYYLIGNFKISETILVSWGIMAFLAIASYFLTRNLKKVPT
                     SKVQIFLEFAVGGLAKMVTDVMGKNTVKRMPYMIPYIGSLFLFFACSNLSPLLGLRSP
                     TTDLDTTAAWAMITFFMIYFAGVKFKGLSYFKELAQPLAIMLPINIIGELARPISLSF
                     RPFGNILGGTIIMGLFYQLLGFISSLIPGLSIPIGEILIPVPLHLYFDLFAGLLQAYI
                     FIMLTMVFISSAGELD"
     gene            11880..12140
                     /gene="atpE"
                     /locus_tag="ACGI59_001820"
     CDS             11880..12140
                     /gene="atpE"
                     /locus_tag="ACGI59_001820"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HFG9739606.1"
                     /translation="METAIVAAASAIGAGIAVATGIGAGIGQGIAAAKAAEAVGNQPE
                     AKGDITSTLLLGVAIAESSAIYGLVISIILLFVNPFFKYLGM"
     gene            12263..12760
                     /gene="atpF"
                     /locus_tag="ACGI59_001821"
     CDS             12263..12760
                     /gene="atpF"
                     /locus_tag="ACGI59_001821"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFG9739607.1"
                     /translation="MEKALVGITWEFVFQIVNTFIIFLLLRKLLFKPVLNIIESREND
                     IKSDLAEGEKAKNEGLALKKEYESKINFAKDEGQEIIKQATIRAEQKSDDIVNTAKKD
                     ALDIKEKANKDIEQERQKVINEIKNDISNIALLAASKVIEKDLDKSKHEELIENFIKE
                     VGEAK"
     gene            12757..13302
                     /locus_tag="ACGI59_001822"
     CDS             12757..13302
                     /locus_tag="ACGI59_001822"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFG9739608.1"
                     /translation="MINVIANRYAEALFQLGEEENSTDVLFKELEKVVDMMTKVSKDF
                     YKVLKSPLVSKSEKKNLVEIIFSKEVSSNIKNFLKVLVDKDRISYLEDIELAYKELLN
                     KKNNVIDGVAISAIPMSETDIKELEVKLSNKYNKNVTIENVVDKTILGGVLVRIGNEQ
                     IDGTVKTRLDKMKEKLSEVIS"
     gene            13319..14821
                     /gene="atpA"
                     /locus_tag="ACGI59_001823"
     CDS             13319..14821
                     /gene="atpA"
                     /locus_tag="ACGI59_001823"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFG9739609.1"
                     /translation="MNLKPEEISSIIKQQIKNYENKVELTDTGSVLTVGDGIASVYGL
                     EKAMSGELLEFPGEIYGMALNLEEEVVGAVILGDDSEIKEGDIVKRTGRIVEVPVGEA
                     LIGRVVNSLGQPIDGKGPIAYTKTRPVESEAPGIIDRRSVYEPLQTGIKSIDSMIPIG
                     RGQRELIIGDRQTGKTSIVIDTILNQKGKDVICIYVAIGQKRSTIAQLVSSLEKGGAL
                     DYTIVVSATASESAPLQYIAPYAGAAMGEEFMYNGKHVLIVYDDLSKQAVAYREMSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDELGGGSMTALPIIETQAGDVSAYIPTNV
                     ISITDGQIYLQPELFYSGVRPAVDPGISVSRVGGSAQIKAMKKVAGTLKLAYSQYREL
                     AAFSQFGSDLDEDTKKRLAQGERIVEILKQGEHQPIKVENQVMIIYAVINNHLEDIPI
                     DNIARFESELYAFVDNNYPEISRKILGGEDFTHDLTDAINEFKEKFVVEV"
     gene            14849..15727
                     /gene="atpG"
                     /locus_tag="ACGI59_001824"
     CDS             14849..15727
                     /gene="atpG"
                     /locus_tag="ACGI59_001824"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HFG9739610.1"
                     /translation="MAGAGIKAIKTRMGTVESTKQITKAMELVASSKLRKAKEKAEDS
                     REYFNTLYNTVKDIAQTTRGIRNEFLKEREVKNKCYVVVAGDRGLAGGYNSNVLKESV
                     SHMKGKKETVITVGKKAHEFFSKRNYNIAKDINSVEACGYEDATEISQTIIDLYKKGE
                     VDEVYIAYTKFKSALSQEVEILKLLPLVFEKDTDEQGMAVKEEKQNRSKVQYIPSAEA
                     VLSYIVPQFISGTVYGGILESFASEQGARRTAMESATDNANEMLSNLELSYNRARQSA
                     VTQEITEIVGGVEALK"
     gene            15740..17134
                     /gene="atpD"
                     /locus_tag="ACGI59_001825"
     CDS             15740..17134
                     /gene="atpD"
                     /locus_tag="ACGI59_001825"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFG9739611.1"
                     /translation="MANVGKVVQIVGAVLDVKFDSEQSLPNLLNALVIKLGDKEIVAE
                     VAQHIGDDTVRCIAMSATDGLVRGMEVVDTGGPISVPVGDETLGRIFNVLGKPVDGKP
                     APKSAPKLPIHRPAPAYDELETTAEILETGIKVVDLLAPYLKGGKIGLFGGAGVGKTV
                     LIQELINNIAKQHGGISVFSGVGERTREGNDLYGEMSESGVINKTALVFGQMNEPPGA
                     RMRVALTGLTMAEHFRDEQGQDVLLFVDNIFRFTQAGSEVSALLGRMPSAVGYQPTLA
                     TEMGALQERITSTKKGSITSVQAVYVPADDLTDPAPATTFSHLDAKTVLSRQISSLGI
                     YPAVDPLESTSRILDPSIVGKEHYEVARGVQSILQRYKELQDIIAILGMDELSDEDKL
                     IVARARKIQRFLSQSFTVAEQFTGNPGQYVPVKETVRGFKEILEGKHDDLPESAFLFV
                     GTIEDAVRKAKGSM"
     gene            17137..17397
                     /gene="atpC"
                     /locus_tag="ACGI59_001826"
     CDS             17137..17397
                     /gene="atpC"
                     /locus_tag="ACGI59_001826"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HFG9739612.1"
                     /translation="MASEFSLKVVTPSNTFYDGDVEMIVVRTTKGDKGVLKGHIPFVS
                     GIPVGTLKIKKEGSFKEAKIAEGFINVTQDKTVILTESAEWI"
     gene            17859..18239
                     /gene="acpS"
                     /locus_tag="ACGI59_001827"
     CDS             17859..18239
                     /gene="acpS"
                     /locus_tag="ACGI59_001827"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFG9739613.1"
                     /translation="MNIFDIGVDIIEIDRIRKAVDKNNRFLEKIFTDREIEYFNSKNF
                     KAESIAGNFAAKEAISKSIGTGIRLFNFKDIEVLRNEMGKPIVKTYNNLAKMCIDYNV
                     LEIKVSISHSKDYAIANAITIIKD"
     gene            18254..18715
                     /locus_tag="ACGI59_001828"
     CDS             18254..18715
                     /locus_tag="ACGI59_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HFG9739614.1"
                     /translation="MMDKTAKEIMTTDVIVAKQDDSIADVANMLIAEKIGGLPVVDSE
                     NRVVGIISETDILKKEKYIEAPLYINLLQGLIFLDDLKKVEKDIKQVAAYKVGELMSK
                     DIIKVHEDDKFDDVANVMIKKSINRVPVVDDDNKLKGIICRYDIIKALYNE"
     gene            18736..19326
                     /gene="gerS"
                     /locus_tag="ACGI59_001829"
     CDS             18736..19326
                     /gene="gerS"
                     /locus_tag="ACGI59_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="germination lipoprotein GerS"
                     /protein_id="HFG9739615.1"
                     /translation="MRKKWTIVCIMFLALVIIVIGCQKRQSTKEEVYKDFQKQISDMN
                     YYSCKAEVEVVGNKSPHNYVLIHTYKKTDNYKLEVISPKHLKGKSIEYQDDKILVKNP
                     KISDVVELPNTGKNNQYLFVGDFIKNYLQNEEMKVKLSKGHLVLETFIPGDNKYFNKQ
                     VLYVNADTKNPEKMEVLDKEGVPRFTVKYKDFEYRN"
     gene            19341..20498
                     /gene="alr"
                     /locus_tag="ACGI59_001830"
     CDS             19341..20498
                     /gene="alr"
                     /locus_tag="ACGI59_001830"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFG9739616.1"
                     /translation="MQKITVPTWAEINLDNLRFNLNNIKNLLEEDIKICGVIKADAYG
                     HGAVEVAKLLEKEKVDYLAVARTAEGIELRQNGITLPILNLGYTPDEAFEDSIKNKIT
                     MTVYSLETAQKINEIAKSLGEKACVHVKIDSGMTRIGFQPNEKSVQEIIELNKLEYID
                     LEGMFTHFATADEVSKEYTYKQANNYKFMSDKLDEAGVKIAIKHVSNSAAIMDCPDLR
                     LNMVRAGIILYGHYPSDDVFKDRLELRPAMKLKSKIGHIKQVEPGVGISYGLKYTTTG
                     KETIATVPIGYADGFTRIQKNPKVLIKGEVFDVVGRICMDQIMVRIDKDIDIKVGDEV
                     ILFGEGEVTAERIAKDLGTINYEVLCMISRRVDRVYMENNELVQINSYLLK"
     gene            20531..20818
                     /locus_tag="ACGI59_001831"
     CDS             20531..20818
                     /locus_tag="ACGI59_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HFG9739617.1"
                     /translation="MYDKSKEKIEVTLPDSLISEVDSIVQMENSNREDFAKAAFQFYI
                     TQKKKIHIKESMKTGYREMGQINLSLAELGMTVEEVSSDDKNEGKIAQGEY"
     gene            20787..21170
                     /locus_tag="ACGI59_001832"
     CDS             20787..21170
                     /locus_tag="ACGI59_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFG9739618.1"
                     /translation="MKGRLPKVSTNCEIKRGDLYYADLSPVVGSEQGGVRPVLIIQND
                     VGNKYSPTVIVSAITSQINKAKLPTHVEISSNEYGLNKDSVILLEQIRTIDKKRLREK
                     IGCLDENMMIKVDDGLQISLGLFTF"
     gene            21341..22168
                     /locus_tag="ACGI59_001833"
     CDS             21341..22168
                     /locus_tag="ACGI59_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFG9739619.1"
                     /translation="MRDHKGLNEIARIIRRDIVSMIHRAKSGHPGGSLSVVEILTALY
                     FDEMNVDSSNPKMEDRDRFVLSKGHAAPALYATLAEKGYFDKEELNGLRKIGRMLQGH
                     PDMKGTPGVEISTGSLGQGFSVACGMAMASKLDNAPWNVYTLLGDGEVQEGIVWEAAM
                     SAAHYKLDNLIAFLDNNGLQIDGDIESVMSLGSIVDKFKAFGWNVIEIDGHDFDQIFA
                     ALDIAKSTVKKPTMIVAKTIKGKGISFMENQAGWHGTAPSDEELEKALLELGGADNE"
     gene            22161..23102
                     /locus_tag="ACGI59_001834"
     CDS             22161..23102
                     /locus_tag="ACGI59_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HFG9739620.1"
                     /translation="MSKMATREAYGKALVKLGQINDNVVVLDADLSKSTKTHDFYKSF
                     PDRFFNMGIAEQNLIGAACGLSTAGKIPFASTFAMFATGRAFEIIRNSVCYPKLNVKI
                     CATHAGLTVGEDGASHESVEDIAIMRAIPNMTVLVPADGVETEKIIFEIAKYNGPVYV
                     RLGRSSVPVLFDEDYKFEIGKGTVLREGKDVSIIACGIMVNEALLAQEKLQEEGISAR
                     VINMSSIKPIDKDLILESAKETNAIVTVEEHSIIGGLGSAVSEVVGESCPTIVKKVGI
                     KDTFGESGTPNELLKKYELTCDDIIKTVKEVIIAKRM"
     gene            23525..24619
                     /locus_tag="ACGI59_001835"
     CDS             23525..24619
                     /locus_tag="ACGI59_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9739621.1"
                     /translation="MSEKVNVKTVISFAGAYVATVIGSGFATGQEIMQFFSFYGFAGI
                     IGGIISMVLFSWMGASVMSKGKELQLKEPIKIYRVYCGKYLGIFFEWFGPLFLFGVFV
                     VMISGAGATLTEYYGLNPFVGRVGMAVVALLTVSLGLDKLSKILGGIGPIIIIFTLLV
                     GGISLAKNIGNIGEAAQVLSTVKIAKPVPNAYLSGVIYTTYNTIVVMAFLTGLGASAA
                     NKKEAICGGILGGVALMAAAIMMHLAILSDIGNLYSKAIPSLFLADKISPVIGVLFSI
                     ILILGIYTTAVPLLWSVTNRFVDDKHPKFKLITLIAAILGLIGGFLPFDKLVGILYPY
                     TGYMGVIILVCVLYRQITKTSGYQEGISDK"
     gene            24845..25261
                     /locus_tag="ACGI59_001836"
     CDS             24845..25261
                     /locus_tag="ACGI59_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9739622.1"
                     /translation="MNVKFNIKGIIYGVIALVFVIGGFIFIPSMFVEKSKPIDYTVLQ
                     RNSIPEKILDVMDKYTNEERALAAKIDNKIYIIVTRGNNKYGIEMDKIESVVEEGKDV
                     LRVKIKYKDKEDSFPYIVVETNMSELPDRIELNATK"
     gene            complement(25443..26012)
                     /locus_tag="ACGI59_001837"
     CDS             complement(25443..26012)
                     /locus_tag="ACGI59_001837"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFG9739623.1"
                     /translation="MKKEFRKKVIESRKNKSANFVLSNSNLITEKLLQMEDIRNATNI
                     MLYLNFNNEVQTDNLIKKLLSLKKIVSSPITIKENHTLIPTQITDLKEGLRIGAYGIR
                     EPNEKSPTIDIKALDVVIVPAVAYDIHCYRLGYGGGFYDRFLENLRKDAITIGIAFDF
                     QIFDSIPKETHDAQLDYIVTETRIITPNN"
     gene            26166..27323
                     /locus_tag="ACGI59_001838"
     CDS             26166..27323
                     /locus_tag="ACGI59_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HFG9739624.1"
                     /translation="MYLVISKKKAIFLGVILVIITAMVTSAFQLTLGNKVVISKELYE
                     DYKKYDKLLGLESIIKQDFYKKVSDTDLVNGAAKGLFLGTNDKYSGYYTKDEMENLIN
                     DSEGSYVGVGMYIGASKDGGLVVVPMKDSPAEKAGVKSGDKLVKVNGKSVSYKNSDEA
                     VRMMKGKKGKTVELTILREDKQLNFKVKTDQIIEKSIESKVIDNDLGYIEITQFISST
                     YTDFDKALKELKAKNIKGLVIDLRNNPGGMLDICKEVADELIGEGTIVYTKDNKGNTE
                     YLKSDKEKLGLPIVVLTNGESASAAEILTAAIVDNKEGISVGTTTFGKGLVQSVVRLK
                     DGTGYKLTTAQYFTPNGDYINEKGIKPTIEEKDENKQLDVATKWLREQIDK"
BASE COUNT        10907 a         3030 c         5312 g         8431 t
ORIGIN      
        1 agacttaact gtttaaataa aacttttctt tttcatgcct tcaatttcca aaataagtat
       61 gtatataaat gaatatatag atgaggagga tttttttgat tttagatgat aaagtattag
      121 aaggattagg tagtaagacc ttagttgagc tacgagagat agctaaagaa ttaaaaataa
      181 aatcaattac tacatataag aaaaatgagt taattgagat tataaattcc aaaagtaaaa
      241 atgaggaaaa agtaaacgaa gagactaaag aaaaaaatga agatattaaa gaagacataa
      301 agaatagaaa agaaaatata gagcgtgaaa gtatagaaaa agaagttgaa tataagagcc
      361 ctaccaaatc atacaacaag aaagaaatag atacccaaaa tatttcccaa aaccaagaac
      421 atagaaatca aagaaatgac tataataaga caacgagtgt aaatgatagt aataagtcat
      481 caaatatatc taatagaatg gtcagaaata ataataaaaa ctattatatg cctaaacaag
      541 ttgatgaatc taagatagta gatgaattta acacatcaaa agaggatgaa gtagtaggtg
      601 tacttgaaat tctgccagat ggatttggtt ttttaagagg ttcaaattat ttgtctactg
      661 aaggagatgt ttacgtttca ccatctcaaa taagaagatt taatatgaaa acaggagaca
      721 agattaaggg aataactaga catccaaaaa gtggagagaa atttagagca cttttatatg
      781 ttcaaaaaat aaatgatgaa aaccctgaca cagctataca aagaaatgcg tttgagacat
      841 taacaccaat atttccagaa gaacgactta cactggaaac taatagaaat gaaatagcta
      901 caagaattat agatttaatt tcacctatag gaaaaggaca aagaggattg atagtagcac
      961 ctccaaaagc tgggaaaaca gttcttttaa aaagcgttgc aaatagcata gctaaaaatc
     1021 atccaaatgt tgaacttata gtactcctta tagatgaaag accagaggaa gttactgata
     1081 tgaaagaatc tattgaagga gacgttatat attccacttt tgaccaagta tcaagtcatc
     1141 atgtcaaggt tgcagagatg gtgttaaata gagctcaaag attagttgaa catggtaaag
     1201 atgtagtaat tttactagat agtataacca gattggcaag ggcatataac cttacaatat
     1261 ctcctacagg tagaacttta tctggaggta ttgaccctgg tgcacttcat ggacctaaaa
     1321 agttctttgg tgcagcaagg aatattagac agggtggttc tttgacaata ttaggaacag
     1381 cattggttga aactggttct aggatggatg atgtaatatt tgaggaattt aaaggtacag
     1441 gaaatatgga gctacattta gatagaaaat tagcagaaaa aagaatattc cctgcaatag
     1501 atatttacaa gtctggtaca agaagagatg atttattgct tgatgatgaa gaaaagactg
     1561 ctctttggag attaagaaga gaaatgagta ataattcagt aatggaaatt acagataaag
     1621 tcattgaact tataaagaga actaaggaca ataaagagtt tgtaaaatca ataaagaatt
     1681 tgtaaaatca ataatacaaa tgcaaatatg gattatataa actaatagaa aaatcacttg
     1741 aatggcataa taagttgtgc tataataatg tagttgataa tgtaaaaaga aagttaatat
     1801 aattaatttt tttaaataaa gagaagaggt gactataatg caaaaggaaa tacaaccaaa
     1861 atacaatcca gttgaagtgc gttgtgcttg tgggaacaca tttgttgctg gttcaactaa
     1921 ggacgaaata aaagttgaaa tatgttcaga gtgccatcca ttctatacag gaaaacaaaa
     1981 gaacatagaa aaaggtggaa gaatcgacaa attcaagaaa agattcaaaa tggactaatt
     2041 tttataaaag tggagttgga ggaagccaac tcctatttta ttacaaaaaa acattcatat
     2101 tctactttaa attgtctttt tgtattataa taataaatat gtaaaattga gtattctaaa
     2161 tacaataaaa tgaggtgagt ggatggcaaa acaagctgta ggtgggcaag ctgttattga
     2221 aggtgtaatg atgcagtcaa aagacaaaag agcggtagct gttagaaaaa gtgacggaga
     2281 aatagcacta aaagaagata gaataaaaag ttgggtaaga gataagaata tagataagat
     2341 accttttgtt agaggttctt ttgttatgat agatacaatg attcaaggca taaaaagttt
     2401 aaacttttct tcagagtttt ttatggaaga agctgaagag gataaatttg atttatttat
     2461 aaagaaaatt tttaaagata aggcaaatga tataattata atattttcat tggtcatagc
     2521 aatgttactt tctgctggat tattcatttt tatacctaca ttggtaggag gagctttttc
     2581 taaagtaatg cctaatgatt ttatgcttaa tttaatagaa ggaatcataa ggatagctat
     2641 attatttgca tatatagtat tgatttcacg aagtaaggat atagaaagag tctttcaata
     2701 tcatggagca gaacataaat ctatatattg ctatgaaaat gatttagaac ttacagtaga
     2761 gaatgctaga aaatttaaaa gattacatcc tagatgtggt acaaatttcc tatttatagt
     2821 tatggctgta tcaataatat tgtttgcatt ttttggttgg ccaaatccaa ttcttagaat
     2881 atttatgaga attatatgtg tgcctatagt agcaggttta tcttatgagg taattagagt
     2941 tcttgggaaa tatgataatg gatttactaa aattattgca tatccaggaa tgatgttgca
     3001 atattttaca acaaaagagc ctgatgatga gcaattagag gttgctttgg aagcattgaa
     3061 agcagtagta gattagagaa aaggatattt gttatgacga taaaagatat aattattaaa
     3121 tattcagata aactaaaaga tataagtgat actccaagat tagatacaga gttattgtta
     3181 caaaaaacat tgggtgttga tagattatat attcacttaa atctgaataa agagctaact
     3241 gaagaacaaa aaacaaaatt tatgggtttt gcagaagaaa gattaaatgg aagaccaata
     3301 gcatatatag ttgaaaatag ggagtttatg ggattagatt tttttgttaa ggaaggcgta
     3361 ttgattccaa gaccagatac agaaacttta gtagaagaaa taattgaaat atgtagagaa
     3421 aaaaaagatg taagtatatt agatatagga actggttctg gagctattac aataagttta
     3481 gcaaagtaca ttgaaaattc taaaatcatg tcttttgata tatcagagac agcattagaa
     3541 atagctaaaa aaaatgctat tataaatgaa gttggtgaaa aaataaagta tattaattct
     3601 gatttattta cagcaataag tgatagtaac ataaaattcg atatcatagt ttcaaatcca
     3661 ccatatatta aaaaacaaga tatagaaact ctgcacaaac aagttaaaga ttatgaacca
     3721 tataatgctc ttgaaggtgg agaagatgga ttagactttt atagaagaat aacagaacaa
     3781 ggtaagaaat atttaaataa gtgtggaata ttagcatatg aagttggtca taatcaagct
     3841 gaagatgtaa taaatattat gaaaagtaat ggatataaaa aaatatacac aaagaaggat
     3901 attcaaggca ttgatagagt tgttataggt tacaatatat gatataatca acaattgata
     3961 tgttacattt aatcgaggtg aaagtatgtt aaaaaaatta gaggtattgg aagatacata
     4021 taaagactta agtgaaaaaa taggtgatcc ggatgtaata aatgatcaaa aagtttggca
     4081 gaaatatata aaagaacatg ctgacttgga acctattgtt atgaaataca gagagtataa
     4141 aagcgtttta gatagtataa aagaatcaaa agaaatttta caagaagagt cagatgaaga
     4201 attaagagaa ttagctaaaa tggagttagc tgagatggaa gaaaaagtag cacctctgga
     4261 ggaagaaata aaaatactat tattgcctaa agaccctaat gatgataaga acgttatagt
     4321 tgaaattaga ggtggagctg gaggagatga agcagctcta tttgctggtg atttatttag
     4381 aatgtactca aggtatgctg aaagaagaag atggaaaata gaattattga gtgcaagtga
     4441 tactggagtt ggtggatata aagaagtatc ttttatgata aaaggtaaag gtgcttattc
     4501 aagattaaag tatgaatctg gagttcatag agttcaaaga ataccatcaa ctgaatctgg
     4561 tggaagaata cacacatcaa cttctactgt tgcagtatta ccagaagttg aagatgtaga
     4621 ggttgaaata aatccaaatg atttaagaat agatgttttc cgttcttcag gtaatggagg
     4681 acaaagtgtc aatactactg actctgctgt tagggtaaca catataccta caggtgaagt
     4741 agtatcttgt caagatggaa aatcacaatt aaaaaataaa gagcaagctt taaaaatatt
     4801 aaaagctaga ctttacgata aagcattggc agaacaacac aaagatatag cagcagaaag
     4861 aaaaagccaa gtcggaacag gagacaggtc agaaagaata agaacatata atttccctca
     4921 aggtagaata agtgaccata gaataaattt aactttatat aagttagatg cattcttaga
     4981 tggagatata gatgaaatga tagatgcttt aattactgtt gaccaaacag aaaaaatgac
     5041 tgcaatataa gtaatgtttt aaagaagctg tttcttccta gaaattattt tctattttga
     5101 aacagctatt ttgcatttta ggtcatgtta tcatattcta ttgtatataa ttgttatata
     5161 caagtatagg agggttatat gatactaaaa gtaacaataa ttgggctttt ggcaggtgtt
     5221 atagggacag gattaggagg agtaatatca gccattttta aaagagaggt cgataaatat
     5281 ctaagttttt ttatgggatt atctggtgga ataatgttgg ctgtagtggt gtttgattta
     5341 atgaaagaat ccatggacaa aatggggata atcaatacag ttatatttac gtttgttgga
     5401 gctctcataa ctatgtacat aaaaacaaaa ttagatgtgt ctggaaatat ggcatctgga
     5461 tatcttatat tcataagtat attactacat aatttaccag aaggactagc cattggttct
     5521 tcttttatgt caactgaaag tttaggtatt acacttgcta tagttatagg tctacataat
     5581 ataccagaag ggttagctat ggcattaggc ttagtttgca ataaaatgaa actaagtaaa
     5641 gttattttgt ttactgtaat agctggatta cctatgggtc taggaagttt tttaggtgtt
     5701 tattttggag gagtatttac ttctcttata ggagtatttc ttgctacagc aggaggtact
     5761 atgatgtatg ttgtattgga agaaattttt ccacattcaa aaagtgtgta ttgtataatt
     5821 ggtttcttgt taggtacaat gattgtaaat tatatataaa attaaaatga aagcaggcat
     5881 acaaaagatg aaaactatta taagtaatat tgatataaat aatattgatt atgaggaaat
     5941 aaaaatacaa gcaaaacttc ttagagaagg taaaacggtt atttttccaa ctgagacagt
     6001 atatgggttg ggtgcaaatg cattagatga aaatgcagta aagaagattt atgaagctaa
     6061 aggaagacct agtgataatc cattgatagt ccatatatat gaaaaagaag aagtatatga
     6121 cttagccaaa gatataagtg ataaagctaa acttgttata gaaaagcttt ggccaggacc
     6181 tataacaatt atattaaata aaaaagatat aattccatac aaaactagtg gagggcttga
     6241 tacagttgca ataaggatgc cttctaatgt tattgccagg gcaataataa aagaagctgg
     6301 tatacctatt gcagctcctt ctgcaaatat ctcaggtcgc ccatctccaa caaaagcaaa
     6361 acatgtatat gaagagatga atggaagagt ggatggaata gttttaggag gagacagtaa
     6421 ttttggattg gagtcaactg tattggattt aacagaagag actcctatga tactgagacc
     6481 aggaagtata actaaagaag ttttagagga acttcttgga gaagtaaagc ttgacccatc
     6541 attaagtaaa aaagaagata atcaaaaagc taaagctcct ggaatgaaat ataaacatta
     6601 ttctccaaat gcagatgtat atataatatc tggaaaaaga gaaaatgtag caacaaagat
     6661 taatgatatg ataaaatcta atagagacaa aggtctaaag acaggtgtaa tgtgtattag
     6721 tagaaaccga gagttttatg atggagaagt aatcgattta ggaaactctc ttgaagaagt
     6781 tgcaagtaat ctgtttgatg ctcttataga aatggacaaa aagggtgttg atgtcatata
     6841 ttcagaagag ttccccaaga ctggagttgg acaagcaatt atgaatagac tactgaagtc
     6901 agcaggatac aaagtgataa aggcttaaga gggagttgta aaatatgaat atacttattg
     6961 tgtgtacagg taatacttgt agaagcccta tggctgaggc tatactaagg aaagcaatta
     7021 aagaatctgg aagaagtatt gaagaatatt ctatatcatc agcaggtata tctacagcaa
     7081 atggaatggg tgcttctgaa aattctatag aagtactaaa agaaatcggt atagacttat
     7141 caaatcatag aagtaaagta attactaaaa aattaattga tgaatcagat ataatactaa
     7201 caatgactaa atctcataaa gaaattttag tacaagcagt tccaaaatgc aaagaaaaag
     7261 tgtatacttt taaagggttt gcaaataaaa atgaggaaga tatatctgac ccatttggtg
     7321 gaaatttgga tatttataga agcactatga gagaaataat gtattctgtc aacgaaatag
     7381 taaaaaaaat atgaaacgga ggataaacaa atgaagatag gattaggctg tgaccatgga
     7441 ggatataatt taaaaaaaga aataataagc tatttagaaa gtaaaggtat agagtgtgta
     7501 gattatggaa ctaataatgc tacagattct gttgattatc ctgtttatgg agaaatagtt
     7561 gcaaattcag ttataaataa agaagtagat tatggaatat tatgttgtgg tacagggata
     7621 ggaatatctt tagctgctaa taaggttcct gggataagat gtgcagttgt atcagatgta
     7681 ttttctgcaa aaatgtcaaa agcacataat gatgcaaata tgttatcatt aggcgaaaga
     7741 gttttaggaa aaggtctagc acttgagata gttgaagcat ggattaatac ggattttgaa
     7801 ggtgatagac atgctagaag agtaaacatg attaaatcaa tagaagaaaa acataataaa
     7861 taataaaaat tacagtttta tcaggaggaa ttatcaaaat gagcaaagtg gtagagacaa
     7921 accatccatt aatacaacat aaattaactt taatgagaga taaaaatact ggttctaaag
     7981 actttagaga gcttttaact gaaattgcaa tgttaatggg gtatgaaata acaaaagata
     8041 taccattaaa agatgtagaa atagaaactc ctattcaaaa aacatcatct aaagtagttg
     8101 caggtaaaaa attagcaata attccaattt taagagcagg tcttggaatg gtagatggat
     8161 tagtgagctt aatgccagca gcaaaagttg gacatgtagg actatataga gacccagaaa
     8221 cattaaaacc agttgaatat tattgtaagc ttcctcaaga cataggagaa agagatataa
     8281 tagtagttga ccctatgctt gcaacagggg gttctgctgt agcagctata gatttactta
     8341 agtctaaagg agctaaaagt ataaaattag ctaatttagt agctgcacca gaaggtatag
     8401 cagaagtgca aaaatatcat gatgatgttg atatatatgt tgcatcagtt gacgaaagat
     8461 taaatgaaca tggatatata attcctggtc ttggtgatgc tggagataga ttgtttggaa
     8521 ctaaataaaa ataaataact ataaatattt attagggact ttcttatata gtgtatataa
     8581 ttttatgtaa gaaggttctt ttttatatat tttttctcaa aataggaatt attgaattga
     8641 taaatttctc gaattgagaa taaaaagttt ttaccaatta aaaaagattg cttattttac
     8701 aaggttttat agaatagtag tctattttaa tgtggcatgt tagttgctta cgattatagg
     8761 tattaagtgt attagttaaa ttgagaattg aatattaata atattattgg gagtggtaat
     8821 acatgaagaa ttgtatagta gtaagagggg ctggagattt agcaacagca gttattcata
     8881 aattacacaa cagtggtttt aaagtagttg cgttagaaat agaagagcca ttagctataa
     8941 gaaggcaaat ttctttttgt gaggctgttt atgaaaaaaa agtaactgtt gaaggtgtag
     9001 aatgtatatt ttgcaatact gaaaaagaaa tagaggaagt tttatctcaa cataaagtag
     9061 ccttgattat agacccaaaa ggagaaaaat tatctaaatt aaatccaatc gttttggttg
     9121 atggaatact cgctaagaaa aacttaggta caactaaaga tatggcacca cttacaatag
     9181 ctttaggtcc tggattttgt gcaggagaag atgtagatgt tgttatagag acaatgagag
     9241 gtcataatct aggaagaata ataactaatg gtagagcaga aaaaaataca ggagtaccag
     9301 gagcaataaa aggtttttct aaagagagag ttatgcattc acagtatgat ggcataatgg
     9361 aaaatgtatg taagataggt gatatagttg aaaagggaga cctactttgc tatattaata
     9421 attatgaaaa aaaacatgaa gttagagcaa caatatctgg agtccttaga ggtttattaa
     9481 aggatggagc aaaggtaaac aaaaatttaa aaatactaga catagaccct agaaaagaag
     9541 aagttaagaa tgcttttaca atatcagata aagctagatg tatagctgga ggggttttag
     9601 aagccatact aaacaatggg atttatcccc aaaattaatt tatattagat aaagctatta
     9661 atattttatt aagatgatat aataaattta atagattaaa gagagcatat gacgggtatt
     9721 taaaatttaa atggtaaaaa tgctcttttt ttattggaca ttcaattaac atgtgtatat
     9781 aatataaaaa taagtattta tttaggttga aatctaataa atatataata ccagtacata
     9841 agcaaagagg agaaaagtaa attatgagac catcatggga tgaatacttc atggaaattg
     9901 cagaagtagt gaaaaagcgt tcaacttgta ttagaagaca agtaggagct gtaattgtaa
     9961 gagacaaaca aatattaaca actggatata atggttctcc gagaaatctt gaacactgtg
    10021 aaaatattgg atgtaagagg caggaattaa atattccctc aggagaaagg catgaattat
    10081 gtagagcatt acatgctgaa caaaatgcga taatacaagc tgctcataat ggtattagtg
    10141 tagatggggc aactttgtat gttactacaa gaccttgtgt tttgtgtgca aaaatgtgca
    10201 taaatgcagg tatattgaaa attgtttatg aaggtgatta tccagatgat atgtctacag
    10261 aattattaaa agaagcagga atagaactca taaaatttta gtggataaaa tttatatgac
    10321 aataaatgtc aaattatgac actgaaatac aatggtatca aatgattaaa agaaagtatt
    10381 tgaaaaatca tcagattagt agtaagatat attttataat taaataaatt tatgagatag
    10441 attaattaca tgttatttat tttataataa tgagggatac tatttatgag taaaagaaat
    10501 acacatacag aaattgcaaa gttgtttagt ttaataagtc aaataggatt gatgattgta
    10561 atatcaatat taggatgtac tttcttagga aaatttttag atagtaaatt gaatactact
    10621 ccagttctga caataatatt tttattgttg ggtgttggag gtgcttttag tggtgtttat
    10681 aaaactctaa tagtatatac acgaaggaag tgatatattg gatataaaat tacttaacca
    10741 ggtaaggtca gtaacaaaag gtatagtaat atttgatgta gttttaattg tgctaatgtt
    10801 gataacatca acattaagta aaccagtatt attaggactt atatttggct ctataatagc
    10861 tatgttaaat tttagattat tggcgatatc actggaaaaa tcagtgacat ttcctgctgg
    10921 gaaggcacaa gcttatgcat cagcacaata tgctgttaga ttatttataa tggcagttgt
    10981 tctaattgca tcagctacag taccacattt aaatataatc ggaacagcat tgggattatt
    11041 gagcacaaag tttgtcatat tatcaaaaaa ctttataaat aaattaaaga ggaaggaggc
    11101 ataagtatga atcaagcgca ttattatctc ataggaaact ttaagattag tgaaacaata
    11161 ctagtaagtt ggggaataat ggccttttta gcgatagcat cttatttttt gactagaaac
    11221 ttaaaaaaag ttccaactag taaagttcaa atattcttag aatttgcagt aggtggtctt
    11281 gctaaaatgg ttacagatgt tatgggtaaa aatacagtaa aaagaatgcc atatatgata
    11341 ccatacatag gaagcttgtt tttatttttc gcatgttcaa atttaagccc attacttgga
    11401 ttaaggtcac caacaaccga cttagacaca actgctgcat gggcaatgat tacatttttt
    11461 atgatttatt ttgcaggagt taaatttaag gggttatcat actttaaaga attggctcaa
    11521 ccacttgcca taatgttacc aatcaatatt ataggtgagt tagctagacc aatatcatta
    11581 agtttccgtc catttgggaa tattttaggt ggaacaataa taatgggtct gttttatcaa
    11641 cttttaggtt ttatatctag tttaatacca ggattatcaa taccaattgg agaaatatta
    11701 ataccagtcc cattacactt gtactttgac ttatttgcag gattgttaca agcatatata
    11761 tttataatgt taacaatggt atttatatca agtgcaggag agttagatta agaaatcaac
    11821 ttatattaag ataaaaataa aataatttaa aaaattaaag ttttaaggag gaaataatta
    11881 tggaaacagc aatagtagcg gcagcatcag caataggagc agggatagca gttgcaacag
    11941 gtataggtgc aggtataggt caaggtatag cagcagctaa agctgcagaa gcagtaggaa
    12001 accaaccaga agctaaaggt gatataacat caactttatt actaggagtt gctatagcag
    12061 agtcttcagc aatatatggt ttggttatat caataatact tctattcgta aatccattct
    12121 ttaaatactt aggaatgtag atttagtcct taaatatttt gagataggtt tataggtaaa
    12181 tcatctcaaa cttataatca tgagatgaac tttatcatct cgtgattata agtagttatg
    12241 atttatggaa aggaggataa ttatggaaaa agcactagta ggtataacgt gggaatttgt
    12301 gttccaaatt gttaatactt ttataatatt tttacttctg agaaagcttt tattcaagcc
    12361 agtattaaat ataattgaat caagagaaaa tgatattaaa agtgatttgg cagaaggcga
    12421 aaaagcaaaa aatgaaggtt tagctctgaa aaaagaatat gagtcaaaaa tcaattttgc
    12481 caaggatgaa ggtcaagaaa tcataaaaca agcaactata agagcagagc agaaatcaga
    12541 tgatatagta aatactgcaa agaaagatgc tcttgatata aaagaaaaag caaacaaaga
    12601 tatagaacaa gaaagacaaa aggttataaa tgaaatcaaa aatgatatat ctaacatagc
    12661 actacttgca gcatctaaag tcattgaaaa agatttagat aaatctaaac atgaagaatt
    12721 gatagaaaac tttataaaag aggtagggga agctaaatga taaatgtaat agcaaataga
    12781 tatgcagaag ccctatttca attaggagaa gaagaaaatt caactgatgt cttattcaaa
    12841 gaactagaaa aagtagtaga tatgatgact aaagttagta aagatttcta caaagtttta
    12901 aaatcaccat tagtttcaaa atcagaaaag aaaaacttgg tagaaattat ttttagtaaa
    12961 gaagtaagtt ctaacataaa aaacttttta aaagttttag tagataaaga tagaatatca
    13021 tatttagaag atatcgaatt agcttataaa gaattattaa ataagaaaaa caatgtaatt
    13081 gatggagtag caataagtgc tattccaatg agtgaaacag atattaaaga acttgaagtc
    13141 aaactttcaa ataaatacaa caaaaatgtt actatagaaa atgttgttga caagactata
    13201 ctagggggag ttctagttag aattggaaat gaacaaatag atggcactgt gaaaactcgt
    13261 cttgataaga tgaaagaaaa gctatctgaa gtaatatctt aagaatggcg gtgaacccat
    13321 gaacttaaaa cctgaagaaa taagttctat aattaaacag caaataaaaa attatgagaa
    13381 taaagttgag ttgacagata caggtagtgt tttaacagtt ggggatggta tagcgagtgt
    13441 atatggatta gaaaaagcta tgtctggtga gttgctagag ttcccaggtg aaatatatgg
    13501 tatggcactt aacttggaag aagaagtagt tggagctgtt atccttggag atgacagtga
    13561 aataaaagaa ggcgatatag ttaagagaac tggaagaata gttgaagttc cagttggaga
    13621 agctttaata ggtagagttg taaactcact aggtcagcca atagatggaa aaggacctat
    13681 agcgtacaca aaaactagac cagttgaatc tgaggcacct ggtataatag atagaaggtc
    13741 tgtttatgaa ccattacaaa caggaatcaa atcaatagat tcaatgatac caattggtag
    13801 aggtcaaaga gagttaataa taggtgacag acaaactggt aaaacatcta tagttattga
    13861 cactatacta aatcaaaaag gaaaagatgt tatctgtata tatgtagcaa ttggacaaaa
    13921 acgttctact atagctcaat tagttagctc tttagaaaaa ggtggagctt tggattatac
    13981 aatagtagtt tcagcaacag catctgaatc agcaccactt caatatatag caccatatgc
    14041 tggagcagct atgggtgaag aatttatgta caatgggaaa catgtactta tagtgtatga
    14101 tgatttaagt aaacaagctg ttgcatatag agaaatgtca ttgttactta gaagaccacc
    14161 aggacgtgag gcatatcctg gagatgtatt ctatttacat tcaagattac ttgaaagagc
    14221 agctaagtta tctgatgaat tgggtggagg ttcaatgact gccttgccta taatagaaac
    14281 tcaagctggt gacgtttctg catatatacc aacaaatgtt atatctataa cagatggtca
    14341 aatatactta caaccagagt tattctattc aggtgtaaga ccagcagttg accctggtat
    14401 atcagtatca agggttggtg gttctgcgca aattaaagca atgaaaaaag ttgcaggaac
    14461 attaaaactt gcatattcac aatatagaga acttgcagca ttctcacagt ttggttctga
    14521 cttagatgaa gatactaaaa agagacttgc tcaaggtgaa agaatcgttg agatattaaa
    14581 acaaggtgag catcaaccaa taaaagttga aaatcaagtt atgataatat atgctgttat
    14641 aaataatcat ttagaggata ttccgataga taatatagct agatttgaat cagaattgta
    14701 tgcatttgta gataataatt atccggaaat atcaagaaaa atattaggtg gagaagactt
    14761 cactcatgat ttaacagatg caataaatga atttaaagaa aagtttgttg ttgaagtata
    14821 atccttttta aaaaaggagg taaattaatt ggcaggagct ggaatcaaag caatcaaaac
    14881 acgtatggga actgttgaaa gtacaaagca aataactaaa gcaatggaat tagttgcatc
    14941 ttctaagctt agaaaagcaa aagaaaaggc tgaagattca agagaatatt ttaatacttt
    15001 atacaataca gtaaaagata ttgcccaaac aacacgtgga attagaaatg aatttttaaa
    15061 agaaagagaa gtaaaaaata aatgctatgt agtagtagct ggagacagag gacttgcagg
    15121 tggatacaat tctaatgtat taaaggaatc tgtctctcat atgaaaggta aaaaggaaac
    15181 agtgattact gttggaaaga aagcacatga atttttttct aaaagaaact acaatatagc
    15241 aaaagatata aatagtgttg aagcttgtgg atatgaagat gcgacagaaa tttcacaaac
    15301 tattattgac ctgtataaaa aaggtgaagt agatgaagtt tatattgctt atacaaaatt
    15361 taaatctgct ttatcacaag aagttgaaat tttgaaattg cttccattag tctttgaaaa
    15421 agatacagac gaacaaggaa tggcagtaaa agaagaaaaa caaaatagat caaaagtaca
    15481 atatatacca tcagcagaag cagttttaag ttatattgta cctcagttta tatcaggtac
    15541 agtatatggt ggaattttgg aatcttttgc atcagagcaa ggtgctagaa gaactgcaat
    15601 ggaatcagcc acagacaatg cgaatgaaat gctatctaat ttagagttaa gttataacag
    15661 agcaagacag tcagctgtaa ctcaagaaat aacagagata gttggtggag ttgaagctct
    15721 aaaataggga ggttaggaaa tggcaaatgt aggtaaagta gtacagattg taggagctgt
    15781 actagatgtt aagtttgata gtgaacaaag cctacctaac ttattaaatg cgttagtaat
    15841 aaagttagga gataaagaaa tagttgcaga agttgctcaa catataggtg atgatactgt
    15901 aagatgtata gctatgagtg caacagacgg acttgtaaga ggtatggaag ttgtagatac
    15961 aggagggcca ataagtgttc ctgttggaga tgaaacttta ggtagaatat tcaatgttct
    16021 tggaaaacca gttgatggga aaccagctcc aaaatctgca cctaaattac ctatacatag
    16081 accagcacca gcatatgatg aactagaaac tacagcagag atactagaaa ctggtattaa
    16141 ggtagttgac ttactagctc catatctaaa aggtggaaaa ataggtctat ttggaggagc
    16201 aggagtagga aagacagttc taatacaaga gcttataaac aatattgcta agcaacatgg
    16261 tggtatttct gtgttttcag gagtaggaga aagaacaaga gaaggtaacg acctttatgg
    16321 cgaaatgagt gagtctggag ttataaataa aacagctcta gtatttggtc aaatgaatga
    16381 accacctgga gcgagaatga gagttgcttt aactggactt acaatggcag aacattttag
    16441 agatgagcaa ggacaagacg ttttactttt cgttgataat atattccgtt tcacacaagc
    16501 tggttcagaa gtttcagcac ttctaggacg tatgccatca gctgttggtt atcagccaac
    16561 attagctact gaaatgggtg cacttcaaga gagaataaca tcaactaaga aaggttcaat
    16621 aacatctgtt caagcagtat atgtaccagc agatgactta actgacccag caccagctac
    16681 aacattctct cacttagatg caaaaacagt tttatcaaga caaatatcat cactgggtat
    16741 atatccagca gttgaccctc ttgaatctac atcaaggatt cttgacccta gtatagtagg
    16801 aaaagaacat tatgaagtag ctagaggtgt acaatcaata cttcaaagat ataaagaact
    16861 tcaagatata attgctatac ttggtatgga tgaattatct gatgaagata agttaatagt
    16921 tgcacgtgct agaaagatac aaagattctt atctcaatca ttcacagttg ctgagcaatt
    16981 tacaggaaat ccaggtcaat atgttcctgt taaagagact gtaagaggat ttaaagaaat
    17041 tcttgaaggg aaacatgatg acttaccaga gtcagcattc ttatttgtag gaacaataga
    17101 agacgctgtt agaaaagcga agggaagtat gtagtcatgg ccagtgaatt ttcattgaaa
    17161 gttgtaactc caagtaatac tttttatgat ggagatgttg aaatgatagt agttagaact
    17221 acaaaaggag acaaaggggt gttgaaaggt catataccat ttgtttcagg tatacctgta
    17281 ggaactttaa agataaaaaa agagggaagt tttaaagagg cgaaaatagc agaaggattc
    17341 attaatgtaa ctcaagataa aactgtcata ttgacagaat ctgctgagtg gatttaataa
    17401 attaatttag agtataaaaa gtcattttga ccaaaaaaac ttttgggtaa aatggctttt
    17461 ttgcgttttg gtgttaataa aataacgata aaattgtaag tttttagtga taataaattc
    17521 acaaaatgtt taaatttact gccaaattat gttgtaatta ataagataaa gaagatttag
    17581 atagctttta tgtaagtgaa aaagtcttca tataagaaat acatataaaa tttttataaa
    17641 atttaaaaaa tttgcttagt tgtgtagtat aataaataat ataatagatt tgtatataat
    17701 attcattaca aataatttta aaagttacag ttttattata atgattatta aattaagata
    17761 agtaacataa actaaataat aatgtgtatt atcgatagta aatttataaa ttcattatta
    17821 attattgaaa gaagtattat aaattttctg gaggaacaat gaatatattt gatattggcg
    17881 tagatataat tgaaattgat agaataagaa aggctgtaga caaaaataat aggtttttgg
    17941 agaagatatt tacagataga gaaatagagt attttaattc taagaatttt aaagcagaaa
    18001 gtatagcagg taatttcgca gccaaggaag ctataagtaa atctataggt acaggaatta
    18061 gattgtttaa ttttaaagat atagaagttc tcagaaatga aatgggaaaa ccaatagtaa
    18121 aaacttataa taatctagca aaaatgtgca tagattataa tgttttagaa ataaaagttt
    18181 caatatcaca ttcaaaagat tatgctatag caaatgcgat aaccataatt aaggactaag
    18241 gagtgattac attatgatgg ataaaacagc aaaagaaata atgacaacag atgtaatagt
    18301 agcaaaacaa gatgatagta tagcagatgt agctaatatg ctaattgcag aaaaaatagg
    18361 aggtctgcca gttgtagatt cagagaatag agttgttgga ataatttctg aaacagatat
    18421 tcttaaaaaa gaaaaatata tagaagcacc tctctatata aatctattac aaggattgat
    18481 tttcttagat gacttaaaaa aagttgaaaa agatataaaa caagtagctg cttataaggt
    18541 tggagaactt atgtctaaag atataataaa ggttcatgaa gatgataagt ttgatgatgt
    18601 agctaatgta atgattaaaa aatcaataaa cagagttcct gtagtagatg atgacaacaa
    18661 acttaaaggt ataatttgta gatatgatat aataaaagct ttatacaatg aataaactga
    18721 agtaggaggt gtgatgtgag aaaaaagtgg accatagtat gtattatgtt cttggcactg
    18781 gttataattg taataggatg ccaaaaacga cagtccacaa aagaagaagt atacaaagat
    18841 tttcaaaagc agatttctga tatgaattat tacagttgta aagctgaagt agaagtagtt
    18901 ggaaataaaa gcccacacaa ttatgtttta attcatactt ataaaaaaac cgataactat
    18961 aaattagaag taatttcccc aaaacattta aaagggaaaa gtatagaata tcaagatgat
    19021 aaaatattag ttaaaaatcc aaagattagt gatgtagtgg agttacctaa tacaggaaaa
    19081 aataatcaat atctttttgt aggagacttt ataaaaaact atcttcaaaa tgaggagatg
    19141 aaagtaaagt tatccaaagg acatttggtt ttagagactt ttattcctgg ggataataaa
    19201 tattttaaca agcaagtatt atatgtaaat gctgacacaa aaaatcctga aaaaatggaa
    19261 gtgttagata aagagggagt gccaagattt acagtaaaat acaaagattt tgaatacaga
    19321 aactaagggg gactaaagac atgcaaaaaa taacagtgcc tacatgggca gagataaatc
    19381 tagataactt aagatttaac ttaaataata ttaaaaattt attagaagaa gatattaaga
    19441 tttgtggagt aataaaagct gatgcatatg gacatggtgc agtagaagtt gcaaaattgc
    19501 tagaaaaaga aaaagtagat tacttagcag tagcaagaac tgctgaagga attgaactta
    19561 gacaaaatgg cataacactt cctattttga acttgggata tactccagac gaagcttttg
    19621 aagattctat aaaaaataaa ataactatga cagtttattc tttagaaaca gcacaaaaga
    19681 taaatgaaat tgcaaaatct ttaggagaaa aagcctgtgt tcatgttaaa atagactcag
    19741 ggatgactag aataggtttc caacctaatg agaagtcagt acaggaaata atagaattaa
    19801 ataaattaga atatatagat ttagaaggta tgtttactca ttttgctaca gctgatgaag
    19861 taagtaaaga gtacacttat aaacaagcta ataattataa atttatgtct gataaattag
    19921 atgaggctgg tgtaaaaata gctataaaac atgtatcaaa cagtgcagct attatggatt
    19981 gccctgattt aagattaaat atggtaagag caggaataat attatatggt cattatccat
    20041 ctgatgatgt atttaaagat agattagaat taagaccagc catgaaatta aaatcaaaaa
    20101 tcggacatat aaaacaagtt gaaccaggtg taggaataag ttatggacta aaatacacaa
    20161 ctacaggtaa agaaacaata gctacagttc caataggata cgcagatgga tttactagaa
    20221 tccaaaaaaa tccaaaggtt cttattaagg gagaagtgtt tgatgtagtt ggtagaatat
    20281 gtatggatca aataatggtt agaattgaca aagatataga cataaaagtt ggagatgagg
    20341 ttatactatt tggagaaggc gaagttacag ctgagcgtat agctaaagac ttaggaacta
    20401 taaactatga agtgttatgt atgatatcaa gaagagttga ccgtgtttat atggaaaata
    20461 atgagcttgt acaaataaac agttatttgc taaaataaaa ccatgatatt ataatctggg
    20521 aggcgatttt gtgtacgata agagtaaaga aaaaatagaa gttactttac ctgactctct
    20581 gatttctgaa gttgatagta tagttcagat ggagaacagt aacagagaag attttgcaaa
    20641 agctgctttt cagttctata taactcagaa aaagaaaatt catattaaag aatctatgaa
    20701 aacgggttat agagaaatgg gtcaaattaa tctgtcacta gctgaattag gtatgacagt
    20761 tgaagaagta tcttctgatg ataaaaatga agggaagatt gcccaaggtg agtactaatt
    20821 gtgaaataaa gcgtggtgat ttatattacg ctgatttaag tccagttgtt ggttcagagc
    20881 agggaggcgt tagacctgtt ctgataatac aaaatgatgt aggtaataaa tatagtccta
    20941 cagttatagt atcagctata acatctcaga ttaataaagc caaattacct actcatgtag
    21001 agataagttc caatgaatat gggcttaata aagactcagt aattctttta gagcaaatta
    21061 gaactatcga taaaaagaga ctgagggaaa aaattggttg tctagatgaa aatatgatga
    21121 taaaagtaga tgatggactt caaataagtt taggattgtt tactttttaa agagggattt
    21181 gtcttttatt agacaatccc ttttttcttt taaatatctc ttaaggttta attttaatgt
    21241 actatgttaa taaaattaaa aataacgcaa aaaaatagca taaaagtgat ataataatat
    21301 ggaattgata taaaattacg aactcaaggg aggtaattgc atgagagacc ataaaggatt
    21361 aaatgagatt gcgcgtatca taagaagaga tatagtttca atgatacata gagccaaatc
    21421 tggacatcca ggagggtcat tatcagtagt tgaaatactt actgcgttat actttgatga
    21481 aatgaatgtg gattcttcaa atcctaaaat ggaggataga gatagatttg tattatcaaa
    21541 aggacatgct gcaccagcat tatatgctac attggctgaa aaaggctatt ttgataagga
    21601 agaactgaat ggattaagga agataggaag gatgttacaa ggtcatccag acatgaaagg
    21661 cacgccaggt gtggaaatat ctactggttc tttaggtcaa ggtttttcag ttgcatgtgg
    21721 tatggctatg gcatcaaagt tggataatgc tccgtggaat gtgtatactt tgcttggtga
    21781 tggtgaggtt caagaaggaa ttgtttggga agctgctatg agtgctgcac attataaact
    21841 tgataattta attgcttttt tagataataa tggacttcaa atagatggag atatagaaag
    21901 cgtaatgagt ttaggttcaa tagttgataa atttaaagct tttggttgga atgtaattga
    21961 gatagatgga catgattttg accaaatatt tgcggctctt gatatagcta aatcaacagt
    22021 taaaaaacct acaatgatag ttgcaaaaac tataaaaggc aaaggaatat cttttatgga
    22081 aaatcaagca ggatggcatg ggacagctcc aagtgatgag gagctagaaa aagctttgct
    22141 tgaattagga ggtgctgaca atgagtaaaa tggcgacgag agaagcatat ggtaaagcat
    22201 tagtaaaatt aggtcaaata aatgacaatg ttgtagtatt agatgcagat ttatcaaagt
    22261 ctacaaaaac acatgatttt tacaagtcat ttccagatag attttttaat atgggtatag
    22321 ctgaacaaaa tttaataggc gctgcttgtg gattatcaac tgctggtaaa ataccttttg
    22381 caagtacttt tgctatgttt gctacgggaa gggcttttga gattataaga aattcagttt
    22441 gttatccaaa gctaaatgta aaaatatgtg ctactcatgc aggtcttact gttggagagg
    22501 atggagcatc tcatgaaagt gtggaagata ttgctataat gagagctata ccaaatatga
    22561 cagttcttgt tccagctgat ggagttgaaa cagaaaaaat tatttttgaa atagctaagt
    22621 ataatggacc tgtttatgtt agattaggga gaagttcagt accagtttta tttgatgaag
    22681 attataaatt tgaaatagga aaaggtacag tcttaagaga aggaaaggat gtatcaatta
    22741 tagcttgtgg aattatggta aatgaagctc tattagcaca agaaaagttg caagaagaag
    22801 gtataagtgc tagagttata aacatgtcct cgataaaacc tatagacaaa gatttaatat
    22861 tagaatcagc aaaagaaacg aatgctattg taactgtaga agaacatagc ataattgggg
    22921 gattaggttc tgctgtaagt gaggtagtag gagagtcttg tccaaccatt gtaaagaaag
    22981 ttggaattaa agatacattt ggagaatctg gtactccaaa tgaattgtta aaaaaatatg
    23041 aattaacttg tgatgacata ataaagactg ttaaagaagt tattattgca aaaagaatgt
    23101 aagagatata aattattcat gtttgttaat aatataatat atttaaaaga gaggtataaa
    23161 gtgttatatt aatttatatc tctctttttt ttatatgata tcaatatatc aaaaatgtaa
    23221 taaaatattc aaatttttac aaaaatagag aaaatattca aatatatatt gactattcag
    23281 aaaaatatga ctaatatata atttatagag aaactaattt tatatataat gactacttag
    23341 ataaaagaat ctaaaaaaat tatgagagga ggagtagggt ataaaatcag attataagtt
    23401 ttaagttatt atactaaaaa gctataaaac agttatcatg tattttatgt taaaggaaat
    23461 tgtaggtagt ttatcgaatt gtttaatcag aagaaaaaat ttcacatggg gaggtaactg
    23521 agatatgtca gaaaaagtaa atgttaaaac agtcatcagt ttcgcaggtg cgtatgtagc
    23581 aacagtaatc ggttctggat ttgcaacagg acaggaaata atgcaattct tctcttttta
    23641 tggttttgca ggtattattg gtgggataat ttcaatggta ttattctcat ggatgggtgc
    23701 ttcagttatg tcaaaaggta aagagcttca acttaaagaa cctatcaaaa tttatcgtgt
    23761 ttattgtggt aagtatttag gaatattctt tgaatggttt ggacctttat tcttatttgg
    23821 tgttttcgta gtaatgattt caggagcagg agcaacttta actgaatact atggattaaa
    23881 tccatttgta ggtagagtag gtatggcagt agtagcatta ttaactgttt cactaggtct
    23941 tgataaatta tcaaagatac taggtggaat agggcctata ataataatct ttacactttt
    24001 agttggtggt ataagtttag ctaaaaacat aggtaatata ggagaagctg cacaagttct
    24061 tagcactgta aagatagcta aaccagttcc aaatgcctat ctatctggtg taatatatac
    24121 tacttataac acaattgttg ttatggcatt cttaactgga ttaggagcaa gtgctgctaa
    24181 caaaaaagaa gctatttgtg gtggtatatt aggtggagta gcacttatgg ctgcagctat
    24241 aatgatgcac ttagctattc tttctgatat aggaaactta tattcaaaag caataccatc
    24301 attattctta gcagataaga tttcaccagt aattggagtt ttattctcaa taattcttat
    24361 attaggtata tatacaacag ctgtaccttt attatggtca gtaactaata gatttgtaga
    24421 tgataaacat cctaagttta aattaataac tttaatagca gctatattag gtcttatagg
    24481 tggcttccta ccatttgata agctggttgg tattttatat ccatatactg gatatatggg
    24541 cgtcataata ctggtttgtg ttctttatag acagattaca aaaacatctg gatatcaaga
    24601 aggaatcagt gacaaataag tattttttaa tattgtaaac tcaagagcta tatgaaaaca
    24661 atgaaaatgt tttcatatag ctctttttat tttttagctc gtttattatg aaatagaaaa
    24721 aacatgtttt ttagttacat tacatatatt tataattatg tataaatata tactaatcaa
    24781 taatttagag tctaagataa gtttataaat catatattta gaatatatgg gaggagtgat
    24841 aagattgaat gtaaagttta atattaaagg tataatttat ggagttatag ctcttgtatt
    24901 cgttataggg ggatttatat tcataccaag tatgtttgta gagaaatcta aacccataga
    24961 ttatacagta ttacaaagaa attcaatacc agaaaagata ttagatgtga tggataagta
    25021 tacaaatgaa gaaagagcat tagcggctaa aatcgataac aaaatataca ttatagtgac
    25081 tagaggaaat aataaatatg gtatagaaat ggacaaaata gaaagtgtgg ttgaggaagg
    25141 aaaggatgtt ttgagagtca agataaagta taaagataag gaagattctt ttccatacat
    25201 agttgttgaa acaaatatga gtgaacttcc agatagaata gaattaaatg cgacgaaata
    25261 agaaggtatt ttataattaa ataactgcat attgtacaat aaagtcatgg tttttattaa
    25321 tgtaagtaaa ttgtatatgg ttcttattgt tgtaaaagcc atgacttaaa atgcttataa
    25381 agtaagatat taacagtagt atttattact atattaaatg gggtctggaa atacttaata
    25441 ttctaattat ttggagtgat aattctagtt tcagtaacta tgtagtctaa ttgtgcatca
    25501 tgagtttctt ttggaataga atcgaatatc tggaagtcaa atgctatgcc gatagttata
    25561 gcatcttttc ttaagttttc taaaaatcta tcataaaatc ctccaccata tcctaatcta
    25621 tagcaatgta tatcatatgc tacagctggt acaattacaa catctaaggc cttaatatca
    25681 attgtaggtg atttttcgtt aggttctctt attccatatg ctccaattct caatccctct
    25741 tttaaatctg ttatttgagt agggattaat gtatgatttt cttttattgt gatgggactt
    25801 gatactattt tctttagaga caaaagtttt ttgattaaat tatctgtttg aacttcattg
    25861 ttaaaattta gataaagcat tatattagtt gcatttctta tatcttccat ttgaagtaat
    25921 ttttcagtga ttaaatttga attactaagt acgaagtttg cactcttatt ttttcttgat
    25981 tcaataactt tttttctaaa ttcttttttc ataaaataat tcctccctta atatgttata
    26041 atgtgtacag caattgtatt taatataaat tttagtataa tttattgtaa taaagcaata
    26101 tatttgggta aatgtaataa aaataacagc tagtcatata ataataatag tttatgtaaa
    26161 aggagatgta tcttgtgatt tcaaagaaga aagctatttt tttaggggtg attttagtaa
    26221 ttataacagc tatggttaca tcagcatttc agttgacatt ggggaataaa gttgtaatat
    26281 cgaaagaact ctatgaagac tataaaaaat atgataagtt gttaggacta gaatctatta
    26341 taaaacaaga tttttataaa aaagtatcag atactgattt ggtaaatgga gcagcaaaag
    26401 gtctatttct aggaacaaat gataagtatt ctggttatta tactaaagat gaaatggaga
    26461 atctaattaa tgatagtgaa ggttcttatg ttggtgtagg tatgtatata ggggcatcaa
    26521 aagatggtgg cttagtagta gttcccatga aagattctcc agcagagaaa gcaggtgtaa
    26581 aatcagggga taaattagta aaagtcaatg gaaaatctgt ctcatataaa aattcagatg
    26641 aagctgttcg tatgatgaag ggtaaaaaag gtaaaacagt tgaactgact atattaagag
    26701 aagataaaca attgaacttt aaagtaaaaa cagaccagat aattgagaag agtatagaaa
    26761 gtaaagtaat tgataatgat ttaggatata tagaaattac ccaatttata tcaagtacat
    26821 atactgactt tgataaagct ttaaaagaat taaaagcaaa aaatatcaag ggattagtaa
    26881 tagaccttag aaataatcct ggtggaatgc tagatatatg taaagaagtt gctgatgagt
    26941 taattggtga aggaactata gtatatacaa aagataataa gggaaatact gagtacttga
    27001 aatctgataa agaaaaattg ggacttccaa tagtagtgtt aactaatgga gaaagtgctt
    27061 ctgctgctga aatattaaca gctgcaatcg ttgataataa agaaggtatt tctgtaggga
    27121 ctacaacttt tggtaaaggg ttggtacaat cagttgtaag attgaaggat ggaacaggat
    27181 acaagttaac tactgctcaa tattttacac ctaatggaga ttatatcaat gaaaaaggta
    27241 taaagcctac tattgaagaa aaagatgaaa ataaacagtt agatgtagca actaagtggc
    27301 ttagagaaca gattgataaa taggatttaa tttttataaa cttatactat aaaataaaga
    27361 acattataaa agagatgtct caaaatgaac tttttagttc atgagggagt ctggaaatat
    27421 ttatgtgtat tctttaaata aaaatttatt ttttaagtga taattaaatt tttacagaca
    27481 gggctatggt tttaaaaatc atagccttat tttgatattt tttaaaaaca cattgcaacc
    27541 cagttgttcc aaatattata tcatttactt ttaaaatatc gtaaactgca tactatatat
    27601 tcattataat ataatttatc taactagaac ctcagtttac taattaaatt agatgcatag
    27661 aataactttt ttaaacattg
//
LOCUS       DAYAPA010000047        27465 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.047, whole genome shotgun sequence.
ACCESSION   DAYAPA010000047 DAYAPA010000000
VERSION     DAYAPA010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27465
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.047"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            <1..860
                     /locus_tag="ACGI59_001839"
     CDS             <1..860
                     /locus_tag="ACGI59_001839"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002844370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HFG9739625.1"
                     /translation="HSLKNIISAKELISKLEKNDNLVVIDCRFDLINRTYGIDSYKKG
                     HIKGSFILDIDKDLSSPTKEHGGKNPLQDPLILKEKLEKMGVDNDTTIVTYDDGDLNG
                     ACRLFFQLKHLGLKNVYVLDGGITSFVKEGGELEEKVNIPSCTGKEIRPNINNDLVVP
                     MEYVKSKLYKKDTVIIDCRANERYQGLVEPAYSKAGHIPSAKNYFCKDLIKSDFENGS
                     LKDIEFLKKFFKDLNNYDEVILSCGSGISACVNSLALRELDIPHKIYIGSFSDWISYD
                     DNEIKTGQE"
     gene            complement(993..2606)
                     /gene="ggt"
                     /locus_tag="ACGI59_001840"
     CDS             complement(993..2606)
                     /gene="ggt"
                     /locus_tag="ACGI59_001840"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HFG9739626.1"
                     /translation="MKFNAYQHKYSSIRNVVYAKNGAVATSTPLASQAGLEILKKGGN
                     AVDAAVATAATLAVVEPTSNGIGGDAYALVWIEEEKRLYGLNSSGFAPENMELKNYNN
                     MKEMPKYGFGAVTVPGIPAAWSELNKKYGKLSLMECLSPAINYAREGYVVSPNVAKVW
                     KKSYELYEKELIGEEFKPWFDTFSKDGKAPEAGDIFICEEQASTLEEIANTQAESFYR
                     GRLADKIDEYSKKFNGAIRKSDLECFYPTWVEPISTEYKGYKIFEIPPNGHGITVLMA
                     LNILKELELEGNIENVEDIHKIIESLKLAFADSKTYVTDIEHMKVKIQELLSQEYAKK
                     RSLLIDNKEALYPTAGEPYCGGTVYLCTADKDGNMVSYIQSNYINFGSGIVIPRTGIA
                     LHSRGNNFNLDPKHHNVVKPFKKPYHTIIPGFLGKEDKAIGPFGVMGAFMQPQGHIQV
                     LTNMIDFGLNPQEALDAPRWQWIKGKEIEVEPEMPKHIIDSLIEKGHEIKVIHDTVDM
                     GRGQIIFKTEQESYICGTESRCDGHIAVY"
     gene            complement(2831..4768)
                     /locus_tag="ACGI59_001841"
     CDS             complement(2831..4768)
                     /locus_tag="ACGI59_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9739627.1"
                     /translation="MTLFRIAMQNVKNSFFNYLMYFISIVFSVFVFFSFKSIEYNEAL
                     SSLGEKTRMSINTSSIVIVVFVFLFIYYSNSFFFNRRRREVGTYSLLGMRKNQIGKIF
                     LYETFLMGIVAILIGIFLGLLFSKLMTMMLVKLMNEMIVVNMNLSIKALVQTLAVFLI
                     IFIVIGIRNTIVIRNKRIIELFNKYPEKYKFKKFIKLKCVLGVLLIAISYLMSISYFI
                     AENIMLSVFILVTIIPGTFLFFSSTMYMIIDAVKKRKSFYYKGQNLIAFSELGFKLKS
                     NSSVLAVIAILIATSVTMLGFTISLYYDIDRNISENYKYSYTINLGNSYVNNEIDKLL
                     YKYKKDNKIIFDKTIELIDKDINIEMYYKNGNSYIEDRTSVDIIRESDFKKLRQYQNN
                     NYEELTSKESVYYVSDSYKKIFFKDFEVEKINLHIKDTNEYDINLFKVQKSILEPEIN
                     SQATFDLIVAKDDVFNQLKLHGNSRLIRLIDIKNEKKELDLTLKLKNIVSENMKFTYP
                     FNFTSSIESYNNLIKLSGLMLFIGIFLSVVFLLCTGSIILFKQLSNIYDDKERYIMLI
                     KLGANNKDIEKIISKQLKVIFLMPLVVGTVHNLFAMSIAQKFIPRSLLVPIIITLVIY
                     FIGYFIYYFLTLKYALNMIKE"
     gene            complement(4755..5522)
                     /locus_tag="ACGI59_001842"
     CDS             complement(4755..5522)
                     /locus_tag="ACGI59_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9739628.1"
                     /translation="MEEILSVENIKKEYGRKGSKHEALRGITFKVYKGEFVGIMGSSG
                     AGKSTLLNIISTIDLPSSGDIYINGKNTIKMKQNELADFRRDNLGFVFQDSNLLDTLT
                     IKENIMLPLSLKNERVSVIENRIKEISKELNIESILDKYPGEVSGGQKQRGAVCRAIA
                     TKPSLVLADEPTGALDSKSARDLLNCLLKLNKDSNKTILMVTHDAISASFCNRILFIK
                     DGIIFTEIVKGESNREFYNKIVNTVSLIGGVNKNDFI"
     gene            5810..6484
                     /locus_tag="ACGI59_001843"
     CDS             5810..6484
                     /locus_tag="ACGI59_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFG9739629.1"
                     /translation="MFKIMVVEDDVSLKNIIAKCLTKWGHDVHQIENLENIIEEFKNY
                     NPELVLLDINLPFYDGFHWCNEIRKISKVPIIFISSRNSNMDVIMGVNLGADDYIQKP
                     FSVDVLVAKVNALLRRTYNFVDNNSNQIIHNGVTLDLSTATINYEDNTIELTKNEIKI
                     LHELMKYKGQIVSRNKLMKKLWDNDWFVDDNTLTVNVNRIRSKLNEIGLEDFIETKRG
                     LGYIIS"
     gene            6508..7512
                     /locus_tag="ACGI59_001844"
     CDS             6508..7512
                     /locus_tag="ACGI59_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HFG9739630.1"
                     /translation="MSFKDFLKDKLGFLVYNFTFLIFTVSVIIFSPVDVFLTDTILYI
                     LVVNVVFLFLYLFISYIKKNKFLDKIKNDTFKINMNDIELARCKNEEKIYLNIIKNHQ
                     YQCNNIINNNIEKFEENKEILNMWVHDVKIPIAIIKLILEQNENLIDEKISDDIDSEI
                     FRIENSVEKILYLSRLEDFHKDFLIQEVNIEKIIRNIIKKYSKYFISKKIILDLNNLN
                     FKVLSDEKWLYFIFEQLLSNSLKYSSLGDNISIYCKRTKNYLQLRVEDTGCGIKKEDL
                     NRIFNKGFTGNNGRNNSKSTGLGLYLVKELCDKLGHKIDVDSEYNQYTKFTITFKCDL
                     "
     gene            7624..8853
                     /locus_tag="ACGI59_001845"
     CDS             7624..8853
                     /locus_tag="ACGI59_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFG9739631.1"
                     /translation="MSFLKKFKNKRFRNNSFKSNNFRNSSFGSSGFKSKNKGFKNNPF
                     KNKRFKIYAGIAVIILLILGVLAYVDSKNTELQANENFIETYTIPENEKIFINGMVVP
                     KQTKDFNISGDYELSDVNVTNGQKVNQGDLLFTAKNPTIIAEIDSLKSQLSQYKKQKI
                     SLSDIAENKDAIASINAQITALSSQIASLDKKAYDRVTAPFDGTVYLNDQTGNPDQPV
                     SFMTIQGLEFYMKGQASEQDLPKMKIDQMVNILVFSTDQKLTGRISFISDKPSTPNTE
                     MGAQQNTLSYYDINIAFDNQEGLVNGFHLQASLEVANSSFKIPASCVLKDNKHSYVFK
                     DLDGILKKQIVDVASQNDDFAVVRGGLEQGDIIIKHPTKEMKEGDPVQGDGVTAGSNN
                     GDTTPNKKVEEVTMDVN"
     gene            8861..9535
                     /locus_tag="ACGI59_001846"
     CDS             8861..9535
                     /locus_tag="ACGI59_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9739632.1"
                     /translation="MLIKLENIQKYYKVGKDELHVLKSLNLEIESGEFVMIMGKSGSG
                     KTTLLNILGFLDVFDEGRYIFDGTDVTNLSENERSVFRNINIGFVFQQFNLIETLNVY
                     QNVELPLIYNKALKKSNREEIVKDKLSSVGLLDKLKQKPLQLSGGQQQRVAIARCLAN
                     DPQIIFADEPTGALDSETSREIMELLTRLNKQGKTIIMVTHDQDLTKYATKVIRLKDG
                     VFTSEV"
     gene            9538..10770
                     /locus_tag="ACGI59_001847"
     CDS             9538..10770
                     /locus_tag="ACGI59_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9739633.1"
                     /translation="MSLLKNSLANLKGHKLRVFVALLWIIIGITSVILVSSIGNGFQK
                     EIKKSVNNVNPNKTTISFESADNTGLTDDMSIFLKPFNAKDLEELSFVEGVERIAPSR
                     DGFNLDSVYSSQASFDKKTTYVDVGPVKKDSKINLICGRDFSLDDEKRKVILLTLQST
                     SEIFENPEDALGHGININGTIFEIIGVLDDSQQNQAGGFFGGYQDMQFTTSLVPKKAF
                     DTLMSQNSYSNEIYQLDLVSSKGYNVNEVANNVIAKLYEMHPGINGSYTTPDPTEQTA
                     YLESINSNVNKYVSIITVVAMFVGGIGVMNIMYVSVMERQREIGIRRAIGAKPRSILF
                     QFLVEAVFITVCGGILGTIVGFTATNYVSKYIGFEAIPSLNSLFYAIVATILTGVVFG
                     LIPAFKASKLDPIKAIYK"
     gene            complement(11056..12321)
                     /locus_tag="ACGI59_001848"
     CDS             complement(11056..12321)
                     /locus_tag="ACGI59_001848"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFG9739634.1"
                     /translation="MILIKNGRVIDPKSQRDENIDLIIKENKIYKIGKFDESDEYEKI
                     IDASGNVVAPGLVDVHVHFRDPGFTYKEDIESGAKSAARGGFTTVICMANTNPIVDNE
                     DTFNYVKEKSKNACINVLQAAAITKGFEGKELVDMEALKKAGVPGFTDDGLPLMDSNL
                     IMEAMIKAKELDVPLSFHEEDPSLVGNPGVNAGKVASELGLKGASSVAEDVMVARDCM
                     LALKTGAKVDIQHISSGVSVDMVRFAKYLGANVVAEASPHHFTLTEEDVLEFGTNAKM
                     NPPLRSKWDRDKIIEGLNDGTIEIIATDHAPHSKEEKDREFIKAPSGIIGLETSLALG
                     ITNLVHKNHLSMMQLIEKMSINPAKLYNLNIGFIEEGAVADIVIFNPEEEWTVESFVS
                     KADNSPFKGKSLYGKVNYTICNGEIVYNA"
     gene            complement(12374..13522)
                     /locus_tag="ACGI59_001849"
     CDS             complement(12374..13522)
                     /locus_tag="ACGI59_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HFG9739635.1"
                     /translation="MKSVSVRSCKTYNYEEVKLSIEKNLEDIGGIDKFVKKGSKVLIK
                     PNLLMKKKPEEATTTHPMVVKVVCEKLLELNCDITIADSPGGPYNQSSLKSIYKASGI
                     EEVANELGIKLNYDVSDVKIHSEKAHVLKYMDIIKPIVDSDYIINLCKLKTHIMATFT
                     GGTKNLYGCIAGLKKAEIHYRFPTEELFCENVLLDICDYIKPTLTIMDGIVGMEGDGP
                     SAGTTREIGVLLASQNPYAIDVVACKIISLLPNRVATIRGAIKRGYIQEDFSDIEILG
                     EDIKDLIIKNYKIPNVSKDLRLFSVKLPKFLNEPISKLITPKPVVRFQDCIKCGKCKE
                     ACPASTIEIGTKGAIIDLSKCIRCYCCHELCPKKAIDVKRNFVFKYVK"
     gene            complement(13613..14158)
                     /locus_tag="ACGI59_001850"
     CDS             complement(13613..14158)
                     /locus_tag="ACGI59_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH peroxidase"
                     /protein_id="HFG9739636.1"
                     /translation="MKKFVCTVCGYIHEGDAAPAQCPVCKVGADKFEEMKGEMVWADE
                     HRIGVAQGVDAEIIEGLRANFTGECTEVGMYLAMSRQADREGYPEVAEAYKRIAFEEA
                     EHAAKFAELLGEVVVADTKENLRVRVDAEYGATDGKLKLAKRAKELGLDAIHDTVHEM
                     CKDEARHGKAFLGLLNRHFGK"
     gene            complement(14381..15325)
                     /locus_tag="ACGI59_001851"
     CDS             complement(14381..15325)
                     /locus_tag="ACGI59_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFG9739637.1"
                     /translation="MQKNKKEGALLKKYFGEIGLIFIAIIWGSGFVATQFALDGGLTP
                     LQIITLRFFLAAIIMNLLFFKQIRANMGKKLLKAGGILGIFLFLAFTVQTIGLMYTTP
                     SKNAFITAANVVIVPFIGFILYRRKLDKIGIISSLVALIGIGILSLEADFSINFGDFL
                     TLICSFGFAFHIFFTSEFAKDNNPMALTAIQFTVAFLMSVVVQTFAGQLKMEAELSGY
                     MGTMYLAVFSTTIGFLFQTICQKRVDGTRTAIILSTEAVFGTIFSIIILKELITAKLV
                     IGSILIFVAIITAETKLSFLKSKKVKLKDSEESSLESI"
     gene            15711..16592
                     /locus_tag="ACGI59_001852"
     CDS             15711..16592
                     /locus_tag="ACGI59_001852"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFG9739638.1"
                     /translation="MVKKRKKKTVTLTILVVIVIGIFSMAWADMSRKKEMKETQERLK
                     AERLEKERIEKEKNEPIIGAKKEAVKEYGYDAKIVWDKLNKYDYSNNGEKIVFLTFDD
                     GPSTTNTPQVLDILKRHGVRGTFFIKGDSLERKGANEILKRTFDEGNAIAHHSYTHDY
                     KKLYPNRSLNLDTFVNELNKTDEAMKKVLGKNFSSNVVRCPGGYMSWKNMEPLGNYLK
                     EKNMASIDWNALNADAEGKKKNAQELFEHAKKSSEGKEMVVLLMHDTYGKQETVNALD
                     QIITYFKDNGYQFKILI"
     gene            complement(16653..17861)
                     /gene="tyrS"
                     /locus_tag="ACGI59_001853"
     CDS             complement(16653..17861)
                     /gene="tyrS"
                     /locus_tag="ACGI59_001853"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFG9739639.1"
                     /translation="MKSIDEQMRIIMKGVDDLIDEKELREKLIKSEKEGKPMIVKLGL
                     DPSAPDIHLGHTVVLRKMKQLQDLGHQIVIIIGDFTGKIGDPTGKSKARKALTTEQVL
                     ANAKTYEEQIFKVLDKEKTIVRFNSEWLAKLNFEDVIKLAATITVARMLEREDFKKRY
                     EGQMPISVHEFFYPLMQAYDSIALEADIELGGTDQRFNLLMGRSLQREFGMESQIVIM
                     MPLIEGLDGKEKMSKSLGNYIGIDEEAGIMYQKSMEIPDELIIKYYNLVTDVHPDEVN
                     KIESQLKEGSVNPRDIKMNLAREIVTLYHGEESAKEAEERFKSVFQKGQIPEDIQTIQ
                     VKEDGFDLIEVLVSNEIVKSKSEVRRLASQGGVKVNGEKVEDLSTIVKESELVVQIGK
                     KKFVKIELVK"
     misc_binding    complement(17934..18186)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(18338..19306)
                     /locus_tag="ACGI59_001854"
     CDS             complement(18338..19306)
                     /locus_tag="ACGI59_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HFG9739640.1"
                     /translation="MNTTKKLTEAALLSSLFIVVTIIAVSTGFGYAIYLDFIVPVFFC
                     IICLKCDLKYTILSGITSLLIISLVLGNLGTAIWASQSVLLGIMCGYLINKPTMVMDD
                     LVYGSVFGLIVMVFIDIYASTLIGYSFMKEFQGYSKWIYFNGYTNFIYYLMIALFPFG
                     MVFCIYLLSLILGNKLHILDSNSLKKFLIFKNFRSLNQFLCCSKKAFYICVSYLAIFE
                     VLKLLNVKVDSVYLKTIFISITYISLYFIIRDSCMSLQNFIIAKFRKLLYARILFLII
                     ILLLFFIFDVTVIGLIIINAFLNKKINIRLSQSNIVNKQINLLIEK"
     gene            19479..19919
                     /locus_tag="ACGI59_001855"
     CDS             19479..19919
                     /locus_tag="ACGI59_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFG9739641.1"
                     /translation="MFKIKHFNDLSLDEFYEIAKSRYEVFACEQKIFSLNDYDDIDKN
                     SYHIFLKENGLICAYARIIPKEYSSYNDVSIGRVLVLSSHRRKGLAKQMMDCAIDFIK
                     VNLHENNITLSAQTYIKNLYLSCGFKEISEVYDEAGIEHIKMRL"
     gene            20246..20473
                     /locus_tag="ACGI59_001856"
     CDS             20246..20473
                     /locus_tag="ACGI59_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HFG9739642.1"
                     /translation="MKNLNDIQVKKTVKVEDILSSGNLRERMLALGLTRGAVIDVVRK
                     GPKNNLTVYKIRGSKIALRQEESSLILVSDI"
     gene            20499..22619
                     /gene="feoB"
                     /locus_tag="ACGI59_001857"
     CDS             20499..22619
                     /gene="feoB"
                     /locus_tag="ACGI59_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HFG9739643.1"
                     /translation="MGLTHNSTKMSSLKDMFDIDNKEDQFVIALAGNPNTGKSTVFNH
                     LTGLRQHTGNWPGKTVATARGNFKYKNTEYALIDLPGTYSLFALSQEEIVARDFICFG
                     NPDAVIVVCDATCLERNLNLVFQVMELTDKVILCINLIDEARKKGITIDKKLLEDSLG
                     IPVILTAARNGSGMDELLDTLNDVSFDKYKLNNKPVRYNENIENVVKSIQPELDNIIP
                     GINSRWLGLRLIDGDESIFESMSNYIDKNSIDAINEVKKKIPDNINKQKIRDEFTKIN
                     YDYAKKLSDECCSNVAKKSTDREEKVDKILTSKIFGLPIMLLLLGTILWITIEGANYP
                     STLLSNLLLGFEPSISGILNSINCPSWLNDMLVLGLYRTLAWVISVMLPPMAIFFPLF
                     TLLEDFGYLPRVAFNLDHLFKKACAHGKQCLTMCMGFGCNAAGVIGCRIIDSPRERLI
                     AILTNNFVPCNGRFPTLIAISTIFFSSVITNSFVSSVATALCITLLIILGVIITLLVS
                     YTLSKTLLKGVPSTFTLELPPYRVPQIGRTLYTSIIDRTIFVLGRAVMVAIPAGVITW
                     IFANIYIGDLSILSHVANFLDPLAKLIGLDGFILLAFILGFPANEIVVPILLMAYLAT
                     GSMIELDSFSALGQVLREHGWTYLTALNVMLFSLLHWPCATTLLTIKKETGSLKWTAL
                     GFLMPTILAFVVCFLTTTVYNLFI"
     gene            complement(22752..23570)
                     /locus_tag="ACGI59_001858"
     CDS             complement(22752..23570)
                     /locus_tag="ACGI59_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFG9739644.1"
                     /translation="MEKQYFTTGEFAKLCGISKQTLIFYDKIGIFSPEYKDKNNYRYY
                     SVYQYDTLDILQSLREIGMSLEEIKEYIQNRTPQLCVKMLKEEEKKIREKIKKLRKIS
                     SKMQNRINITVEGISKMNSEEIYISKKFEEYLVLSSDLSNMSNSDFMMELIDFTNYCK
                     SKNLYQGYELGVIVSNENIKNGDYLSISSFYLKIDKKIKDKKLYVKPEGMYACIKHIG
                     KYEDSYKSYEKLKKYIYENEYKIVGNSYEESILDFFCESNEDNYMTEISIQVSR"
     gene            23789..24061
                     /locus_tag="ACGI59_001859"
     CDS             23789..24061
                     /locus_tag="ACGI59_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9739645.1"
                     /translation="MKIVIGATIRYLIKHKVNYCNKKSLDKLELKYGNIDREKAVKLE
                     IFYQYLIELEYIEMSLLIRRLDTAITSLALVSIITIASYYLIRKNV"
     gene            complement(25114..27207)
                     /gene="pdcA"
                     /locus_tag="ACGI59_001860"
     CDS             complement(25114..27207)
                     /gene="pdcA"
                     /locus_tag="ACGI59_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdcA"
                     /protein_id="HFG9739646.1"
                     /translation="MNKHNFEVILNQLQINIYVTNIHTNEIIFMNKKMKEEYNILDPE
                     GKVCWQVLYPEKNSTCSFCKVLELLKNDKKGVLIKWYEKCNKLNRVFENYDSLITWQD
                     GTVVHMHQSIDIANSTSLNKPIKINEFHEISNNKEEKGVFNFSRDNFDYNSTLLYDAL
                     IRGTDEYIYICNMKTGVFRYSPSQVELFDLPGEIVKNPLVYWKKIVHPEDWNRFYKSN
                     TEIGKNQMDYHTVEFRAKNRSGEYIWLRCRGQLMRDEFGEPSIFAGIMTQLGKQNKID
                     SLTQLLNYHEFMSVFEDKISNPMIEKLCIVLLDIDDFKNVNEMYDRDFGDNIIKTLAQ
                     SVQSILPDNAELYKLDGDEMGILVDNVEENEILTLYNQIQNMIIHLQLWRKYGLNITI
                     SAGCVIYPKHGDTVKELYKCASYSLQYAKEHGKNRLVFFSQEILKNKMYSLEMMRDLK
                     ASINDDFRGFSLRFQPQVDTESHKIIGVEVLLRWTNDKCKAISPLEFIPILEENDMIN
                     IVGAWVLRMALRTFRKWIDYYPFFKVSVNVSAVQILEDTFIEDIVKIIDDENFPYQNL
                     VLELTESHTVQNMSILQFKFKALQDLGIYIAMDDFGTGYSSLEVLKFSPIDIVKIDRV
                     FVKDILKSKFDATFIHFIVAICHDVGIKVCLEGVETQEEYDLVKQIKPDYIQGYLFGK
                     PQTATEIFDLLKLDN"
BASE COUNT         9657 a         4184 c         3371 g        10253 t
ORIGIN      
        1 tacattcttt gaaaaacatt atttcagcaa aagaacttat atcaaaacta gaaaaaaatg
       61 ataatctagt tgtaatagat tgtagatttg atcttatcaa tagaacttat ggtattgatt
      121 cttataaaaa aggacacatc aaaggcagtt ttatcttaga tatagacaag gatttatcat
      181 ctcctacaaa agaacatggt ggtaaaaatc ctttacaaga ccctttaata ctaaaagaaa
      241 aattagaaaa aatgggtgtt gataatgaca ctacgattgt aacttatgat gatggagatt
      301 taaatggagc ttgcagatta tttttccaat taaaacattt ggggcttaaa aatgtctatg
      361 tactagatgg gggaataaca tctttcgtaa aggaaggtgg agaattggaa gagaaagtga
      421 atataccaag ttgtacaggt aaggaaatta gaccaaatat aaacaatgat ttagttgttc
      481 caatggaata tgttaaatct aaattataca aaaaagatac tgtaataata gattgccgtg
      541 ctaatgagag ataccagggt ttagttgaac ctgcttactc aaaagctggt catataccaa
      601 gtgcaaaaaa ttatttttgt aaggatttaa taaaaagtga ctttgaaaat ggctcactta
      661 aagatataga atttttaaaa aaattcttta aagatttaaa taattatgat gaagtaattt
      721 tatcttgtgg ttcagggata tctgcttgtg ttaacagtct tgctcttaga gaactggata
      781 tacctcacaa gatttatatt ggtagcttta gtgactggat tagttatgat gacaatgaaa
      841 tcaaaactgg tcaagaataa ggctcataat ctatataaaa tcagcatatg atagcttaaa
      901 ttttgtaaag gcgtcagtca ttgtagactt tcgcctttac ttattatttc atatactctt
      961 taaatttatt atatatattt tagttgataa tattaataaa ctgcaatatg accatcacat
     1021 cttgattccg tcccacaaat ataactttcc tgttctgttt taaatattat ttgacctcgt
     1081 cccatgtcga cagtatcatg aattactttt atttcatgac ccttttcaat taatgaatct
     1141 attatatgct ttggcatctc tggttcaact tctatttctt ttccttttat ccactgccat
     1201 cttggagcat ctagcgcctc ttgtggattt aatccaaaat caatcatatt tgttaacact
     1261 tgaatatgtc cttgtggttg cataaaagct cccattacac caaatggacc tattgcttta
     1321 tcttctttac ctaaaaatcc aggtattata gtatgataag gttttttaaa tggttttact
     1381 acattgtggt gtttagggtc taaattaaaa ttatttcctc tactatgtaa agcaatccca
     1441 gttcttggta tcacaattcc tgaaccaaaa tttatgtaat tgctctgtat atatgaaacc
     1501 atatttccat ccttatctgc agtacaaagg tatactgtac caccacaata tggctctcct
     1561 gcagttggat acaaagcctc tttattgtca atcaataaac ttcttttttt agcatactct
     1621 tggcttagta attcctgaat ctttaccttc atatgctcta tatcagttac atatgtttta
     1681 gaatctgcaa aagctaattt taaactctct ataattttat gtatgtcttc tacattttct
     1741 atatttcctt ctaattccaa ttcttttaat atgttaagtg ccattaaaac tgttattcca
     1801 tgaccatttg gtgggatttc aaatatttta tatcccttat attctgtact tataggttct
     1861 acccatgttg gataaaaaca ctctaagtca ctctttctta tagccccatt aaatttttta
     1921 gaatattcat ctattttatc agcaagtctt cctctataaa agctttcagc ctgagtattt
     1981 gctatctctt ctaatgtact tgcctgttct tcacatataa atatatctcc agcctctgga
     2041 gctttaccat ctttagaaaa tgtatcaaac catggtttga actcttctcc aataagttct
     2101 ttttcataga gttcatatga ttttttccat accttagcta cattaggaga tactacgtat
     2161 ccctctcttg catagtttat cgctggagat aagcattcca ttaagctaag tttaccatat
     2221 tttttattta attctgacca agctgctggt attccaggta cagttactgc tccaaaccca
     2281 tattttggca tttctttcat attattataa ttttttaatt ccatattttc tggagcaaag
     2341 ccactagaat ttaaaccata caatcgtttt tcttcctcta tccaaacaag ggcatatgcg
     2401 tctcctccaa tcccattgct tgtaggttct actactgcta atgtggcggc tgttgcaact
     2461 gctgcatcaa cagcatttcc accctttttt agtatttcaa gtccagcttg agatgctaat
     2521 ggtgttgatg ttgcaactgc tccattttta gcatacacga catttcttat tgatgagtat
     2581 ttatgttggt aggcattaaa cttcataaat ttcagctcct cgtcattttt tatttgatat
     2641 tctgcttaga tttattatat aacatcaagt aatgtaactg atacaaaatg atataaaata
     2701 tagacttttt cctaacttta attttgtctt taaataaata tcttaaactc aattctagta
     2761 ttttctatct gcacttaaat aagttatttt attcaaaaca tatatctaat tcttttgact
     2821 taaaaataat ttattctttt atcatgttta gagcatattt taaagttaaa aaataatata
     2881 taaaatatcc tataaaataa atgactagag ttatgataat tggtacaagt aatgaccttg
     2941 gtataaactt ttgtgctata gacatagcaa ataaattatg aactgtacct acaactaatg
     3001 gcattaaaaa tataactttt aactgttttg atattatttt ctcaatatct ttattatttg
     3061 ctccaagttt tattaacata atatatcttt ccttatcatc atatatattt gataattgtt
     3121 taaataagat tatactacct gtacataaaa gaaatactac agacaaaaat atgcctataa
     3181 acagcattag accacttaat tttatcaagt tattataact ttcaatactt gatgtaaaat
     3241 taaaaggata agtaaatttc atattttcac ttactatatt ttttaatttt aaagtcaaat
     3301 ccagttcttt tttttcattt tttatatcaa ttaatcttat taatctagaa tttccatgta
     3361 attttaactg attaaatacg tcatcttttg ctacaattaa atcaaacgta gcttgtgaat
     3421 ttatttctgg ctccaagatg cttttttgta ctttaaacag atttatatca tactcatttg
     3481 tatctttaat atgcaagttt attttttcaa cctcaaagtc tttaaaaaat atttttttgt
     3541 atgaatcaga aacatagtac acagattcct ttgaagttaa ttcttcataa ttgttattct
     3601 gatattgtct taatttttta aaatcacttt ctctaataat atctacagac gttctatctt
     3661 ctatataact atttccattt ttataataca tttcaatatt tatatctttg tctataagtt
     3721 ctattgtttt atcaaagatt atcttattat cttttttata tttatataat aatttgtcaa
     3781 tttcgttatt tacataagaa tttcctaaat ttatggtata actgtattta taattttcac
     3841 ttatgttcct atctatatcg taataaagtg atatggtaaa ccctagcata gttacacttg
     3901 tagctattaa tattgctatt acagccaata cactactatt gctctttaat ttaaatccaa
     3961 gttcagaaaa tgcaattaaa ttctgtccct tataataaaa actttttctc ttcttaacag
     4021 catcaataat catatacatc gttgatgaaa aaaataaaaa agttccaggt attattgtaa
     4081 ctaatataaa aactgaaagc ataatatttt cagcaataaa ataactaatt gacattaaat
     4141 aagaaatagc tattaataaa acaccaagta cacattttaa ctttataaat tttttaaact
     4201 tatacttttc tggatattta ttaaataact ctataattct cttatttctg ataactatag
     4261 tatttcttat tccaataact atgaatatta ttaaaaatac cgctagtgtc tgaactaagg
     4321 cctttatact taagttcata tttactacta tcatttcatt cattaatttt acaagcatca
     4381 tagtcataag ctttgaaaat aacagtccta aaaatatacc tattagtatt gcaactatac
     4441 ccatcaaaaa agtttcatat aaaaatattt ttcctatctg atttttcctc attccaagta
     4501 aactataagt tcctacttct cgcctacgtc tattgaaaaa aaatgagttt gaataatata
     4561 taaataaaaa tacaaatacc actattacaa ttgaacttgt attaatactc atcctagttt
     4621 tctctccaag tgaactcaac gcttcattat actctataga tttaaaacta aaaaatacaa
     4681 atacactaaa tacaattgaa ataaagtaca ttaaataatt aaaaaaacta ttctttacat
     4741 tttgcattgc aattctaaat aaagtcattt ttattaactc ctccaattaa ggaaactgta
     4801 tttactattt tattatagaa ctctctgtta ctttctccct ttacaatttc tgtgaatatt
     4861 attccatcct ttataaagag tattctattg caaaaactcg cacttatagc atcatgagtt
     4921 accatcaata ttgttttatt gctgtctttg tttaatttta atagacaatt caacaaatcc
     4981 ctagcagatt tagaatctaa tgctcctgta ggctcatctg caagtactaa tgatggtttt
     5041 gttgctatag ctctacatac agctcctctt tgcttttgtc caccagaaac ttcgccaggg
     5101 tatttatcta atatagattc tatatttaat tcttttgaaa tctcttttat tctattttct
     5161 attactgata ctctttcatt ttttaaagaa agaggcaaca ttatattttc ttttattgta
     5221 agtgtatcca aaagattaga atcttgaaat acaaagccta gattatcacg tctaaaatct
     5281 gccaattcat tttgcttcat tttaatagta ttctttccat ttatataaat atctcctgat
     5341 gaaggtaaat ctattgtaga aataatattt aaaagtgttg atttaccagc accggaagac
     5401 cccataatac caacaaattc tcctttataa accttgaaag ttattcctct tagagcttca
     5461 tgttttgaac cttttcttcc atactccttt tttatatttt caacagataa tatttcttcc
     5521 atagttaata atcacctttc atcacaatta gttatttatc aattctcaca aatatattat
     5581 atatattaaa atggctttta aacatttaat tatattaatt taaggttaca ttttcgtaag
     5641 tattaattat atttcttcaa atttattgca attatgttta aaatataatt tatcaagtaa
     5701 aatatagtta tattaatata attattttag gtaataaggt agaaatattt taatttatca
     5761 agtagaatat agttatgtta atataactat tttgaattag gaggtaagaa tgtttaaaat
     5821 aatggtagtt gaagatgacg tctcattgaa aaatatcata gcaaagtgtc ttactaaatg
     5881 gggacatgat gttcatcaaa tagaaaatct tgaaaatata atagaagagt tcaaaaacta
     5941 taacccagaa ttagttttat tggacataaa ccttccattt tatgatggtt ttcattggtg
     6001 taatgaaata agaaaaattt caaaagttcc tataattttt atttcttcaa gaaattctaa
     6061 tatggatgta ataatgggtg taaatttagg tgcagatgat tatatacaaa aacctttttc
     6121 tgtagatgtc ttagtagcta aagttaatgc ccttttaaga agaacttata attttgttga
     6181 taataactca aatcagatta ttcacaatgg tgttacttta gatttatcaa ctgcaacaat
     6241 aaactatgaa gataatacta ttgagctaac aaaaaatgaa atcaaaattc ttcatgagct
     6301 tatgaaatat aaggggcaga tagtaagtag gaataaactc atgaaaaaat tatgggataa
     6361 tgattggttt gtagatgata atacattaac tgtcaatgta aatagaatac gctcaaagtt
     6421 aaatgaaatt ggtttagaag attttataga aacaaaaaga ggattgggtt atataatcag
     6481 ttaaaacaat tctaagtagg tgaatttatg agctttaaag attttctcaa ggataaacta
     6541 ggttttttag tatacaattt tactttctta atttttacag tatcagttat tatattttct
     6601 cctgtagatg tttttctgac tgatactatt ttatatatat tagttgtaaa tgttgtgttt
     6661 ttatttttat atctatttat tagctacatc aagaagaaca aatttttaga taagataaaa
     6721 aatgatacat tcaaaattaa tatgaatgat atagaattag ctagatgtaa aaatgaagaa
     6781 aaaatatact tgaacattat taaaaatcat caatatcaat gtaataacat aataaataat
     6841 aatatcgaga aatttgaaga aaacaaagag attttaaata tgtgggtaca tgatgtaaag
     6901 atacctatag ctataataaa actcattctt gaacaaaatg aaaatttaat tgatgaaaaa
     6961 atttcagatg acatagatag tgaaatattt agaattgaaa actccgttga aaaaatttta
     7021 tatttatcaa gactcgaaga ttttcataag gattttttaa ttcaagaggt aaatattgaa
     7081 aaaatcatta gaaatattat aaaaaaatac tcaaaatatt ttatttcaaa aaaaattatt
     7141 ttagacttaa ataatttgaa ttttaaggtt ttatctgatg aaaaatggct atacttcata
     7201 ttcgagcaat tattgagtaa ttccctaaaa tactcctcat taggagataa tatatctatc
     7261 tattgcaaaa gaaccaaaaa ttatttacaa ttaagagtgg aagatactgg ttgtggtata
     7321 aaaaaggaag atttaaatcg aatattcaat aagggattta caggtaataa tggaagaaac
     7381 aattctaaat ctacaggtct tgggctttat ttagtaaaag aactttgtga taagcttggt
     7441 cataaaattg atgtagattc agagtacaac caatatacaa aatttactat tacattcaaa
     7501 tgtgatttat agtatcttat tattgatagt ttattttcat taacttatat tcaacttctt
     7561 atagtaattc tatatgtaca tcccagtata taattaagta gtttttataa aaaggaggaa
     7621 gacatgtctt ttctaaaaaa atttaaaaat aaaagattta gaaataacag ttttaaaagt
     7681 aacaacttta gaaatagcag ttttggaagt agtggcttca agtctaaaaa taaaggtttt
     7741 aaaaacaatc cctttaaaaa taagagattc aaaatatatg caggaatagc agtaatcata
     7801 cttttaattc taggagttct tgcttatgtt gattcgaaaa acactgaatt acaagctaat
     7861 gaaaatttta tcgaaacata tactattcct gaaaatgaaa aaatatttat aaatgggatg
     7921 gttgttccta agcaaactaa ggacttcaat atatctggag attatgagtt aagtgatgtt
     7981 aatgttacaa atggtcaaaa agtaaatcaa ggagaccttc tttttactgc taaaaatcca
     8041 actataatag cagaaataga tagtttaaaa tcacaactta gtcaatacaa aaaacaaaaa
     8101 atatcacttt ctgatattgc tgaaaataaa gatgctattg cttctataaa tgcacagata
     8161 actgctttaa gctcccagat agcttctctg gataaaaaag cttatgacag agttactgct
     8221 ccttttgatg gcacagtata cttgaatgac caaactggaa atccagacca accagtttca
     8281 tttatgacta ttcaaggtct agagttttat atgaaaggac aagcaagtga acaagattta
     8341 cctaaaatga agattgacca aatggtcaat atactagttt tttcaactga ccaaaagctt
     8401 actggtagaa taagttttat atctgataaa ccatctactc caaatactga aatgggagct
     8461 cagcagaata cactatctta ttatgatata aacatagcat ttgataatca agaaggatta
     8521 gtaaatggat tccatcttca agcatcactt gaagtagcta attcaagctt taaaatacca
     8581 gcatcttgtg tacttaaaga caacaaacac tcatatgtat ttaaggattt agatggtata
     8641 ttgaaaaagc aaattgtaga tgtagcaagt caaaatgatg attttgctgt agttagaggc
     8701 ggtcttgaac aaggcgatat aataataaaa caccctacta aagaaatgaa agaaggagac
     8761 ccagttcaag gcgatggcgt aactgctggt tctaataatg gagatactac tccaaacaaa
     8821 aaagtagaag aagttactat ggatgtgaat taatttacat atgttaatta aattagaaaa
     8881 tattcaaaaa tattacaaag tagggaaaga tgaattacat gttttaaaat ccttaaattt
     8941 agaaattgaa tctggagaat ttgtcatgat aatgggtaag tctggtagtg gtaaaactac
     9001 tttacttaat atcttaggtt ttctagatgt atttgatgaa ggaagatata tatttgatgg
     9061 aacagatgta actaatttga gtgaaaatga acgttctgtt tttagaaata ttaatattgg
     9121 atttgttttc caacaattta atttaataga aactctaaat gtatatcaaa atgtagagtt
     9181 gcctctaatt tacaataaag cactaaaaaa atctaataga gaagaaattg ttaaagataa
     9241 attaagttct gttgggcttt tggacaagct taagcagaaa ccacttcaat tatctggtgg
     9301 tcaacaacag cgtgtagcca ttgcacgttg cctagcaaat gaccctcaga taatctttgc
     9361 tgacgaaccg actggggcat tagatagtga aactagcaga gaaattatgg agcttttaac
     9421 aaggctcaac aaacaaggta agactataat aatggttaca catgatcaag atttaactaa
     9481 gtatgcaact aaggttatac gacttaaaga tggtgtgttt acttcggagg tgtaatcatg
     9541 agtttattaa aaaattcttt ggccaattta aaaggtcaca agcttcgtgt atttgtagct
     9601 ttactctgga ttataatagg aataacttca gtaatacttg ttagttctat aggtaatgga
     9661 tttcaaaaag aaattaagaa atctgtaaac aatgtaaacc ctaataaaac tactatttcg
     9721 tttgaatcag ccgataacac aggtcttact gatgatatga gtattttttt aaaaccattt
     9781 aatgcaaaag atttagaaga attatcattt gttgaaggag tagaaagaat agctccttca
     9841 agagatgggt ttaaccttga ttcagtttac tcttcacaag catcttttga taaaaaaact
     9901 acgtatgttg atgttggacc agtaaaaaaa gattctaaaa taaatttaat ttgtggtaga
     9961 gatttttctt tagatgatga aaaaagaaaa gtaattttac ttacattaca aagtacaagt
    10021 gaaatttttg aaaatccaga agatgcctta ggtcatggta tcaacataaa tggaactata
    10081 tttgaaataa taggggtatt agatgattct cagcaaaacc aagctggagg attctttgga
    10141 ggttaccaag atatgcaatt tactacttcc ctagtaccta aaaaggcttt tgacacttta
    10201 atgagtcaaa attcttattc aaatgaaatt taccagttag acttagtttc ttctaaaggc
    10261 tataatgtaa atgaagttgc aaacaatgtt atagctaaat tatatgagat gcaccctggt
    10321 atcaatggtt cttatactac accagaccca actgaacaga cagcttatct tgaaagcatt
    10381 aattccaatg taaacaaata tgtatctata ataactgtag ttgcaatgtt tgttggtggt
    10441 attggtgtaa tgaatataat gtatgtatct gttatggaaa gacaaagaga aattggtata
    10501 agacgtgcta ttggagcaaa acctaggtct atattatttc aatttttagt tgaagctgtt
    10561 tttataacag tatgtggtgg aattttagga actatagttg gatttactgc tactaactat
    10621 gtttctaaat atataggttt tgaagctata cctagcttaa atagcttatt ttatgcaatt
    10681 gtagctacta tattgactgg agttgtattt ggtctgattc cagcctttaa agcctctaaa
    10741 ctagacccta taaaggcaat ttataagtag aaattgtata aagattttcg atttttagat
    10801 tagtttatat aaaacatact taaatatata acgcactatt atatatgata gattaggaac
    10861 ttaatctttc caaatataat agtgcgttct ttcataaaaa ggattctctt ccttactata
    10921 tctaaattac caagatgtta tgttctaatt gttcaaacta atacttccaa ttttgattaa
    10981 tcaatctcat tctcctaata tacaatcttt tactttcaat cattgtacac tccgcctaaa
    11041 aaaaataatc ttaaattagg cgttatatac aatctcccca ttacatattg tataattaac
    11101 ctttccatat aaagatttac ctttaaatgg tgaattatca gctttagaaa caaagctttc
    11161 aactgtccat tcttcttctg gattgaatat aacaatgtct gctacagccc cttcttctat
    11221 aaatccaata tttaaattgt acaactttgc aggatttata gacatttttt ctattaactg
    11281 catcatacta agatgatttt tatgcaccaa atttgttatt ccaagagcta aagaagtttc
    11341 aagcccaata attccacttg gagcttttat aaactctcta tctttttctt ctttactatg
    11401 aggtgcatgg tccgttgcta ttatttcaat agtaccatca tttaatccct ctataatctt
    11461 atctctgtcc catttacttc ttagaggtgg attcattttt gcatttgttc caaactccaa
    11521 tacatcttcc tcagtcaatg taaagtgatg tggtgaagct tctgcaacta catttgcacc
    11581 taagtatttt gcaaatctaa ccatatcaac agatacacct gaacttatat gttgtatatc
    11641 aactttagca ccagtcttta gtgccaacat acaatctctg gctaccataa catcttctgc
    11701 tacacttgat gcacctttta atccaagttc tgaagctacc tttcctgcat ttacacctgg
    11761 atttccaact aaagaagggt cttcttcatg aaaactaagt ggtacatcca actcttttgc
    11821 cttaatcatt gcctccataa ttagattgct atccattaaa ggtaatccat catcagtaaa
    11881 ccctggtaca ccagcttttt taagagcttc catatcaact aactctttac cttcaaaacc
    11941 tttagttata gctgctgctt gtaaaacatt tatacatgca ttttttgact tttctttaac
    12001 ataattaaaa gtatcttcat tatctacaat aggattagta tttgccatac atataacagt
    12061 tgtaaatcca cctctagcag cagatttagc tccactttct atatcttctt tatatgtaaa
    12121 cccagggtct ctaaagtgta catgtacatc cactaaacct ggagcaacta cattaccact
    12181 agcatctatt attttttcat attcatcact ttcatcaaac ttgcctattt tataaatttt
    12241 attttctttt ataattaagt caatattctc atctctttga cttttagggt ctattactct
    12301 accattttta attaaaatca tattatttct cctttatatt tttaatttat atagactcat
    12361 ataatattat acattatttt acatacttaa atacaaaatt acgtttgaca tcaatagcct
    12421 ttttaggaca aagttcatgg caacagtaac atcttataca tttacttaag tctataatag
    12481 cacccttagt ccctatctct atagtggatg caggacaagc ttccttgcat tttccacatt
    12541 ttatgcaatc ttgaaatctt accacaggct ttggtgttat aagctttgaa ataggttcat
    12601 ttaaaaattt tggtagcttt acagaaaaca atcttaaatc cttacttaca tttggaatct
    12661 tgtaattttt tataattaaa tcttttatat cttcacctaa aatttcaata tctgaaaaat
    12721 cttcttgtat atatcctctc ttaatagcac ctctaattgt agcaactcta tttggaagta
    12781 aacttataat cttacatgca acaacatcta tagcatatgg attttgtgat gctaataaaa
    12841 cgcctatttc tcgagtagta ccagctgaag gtccatcacc ttccatacct actataccat
    12901 ccataattgt caatgttggt tttatataat cacaaatatc taaaagtaca ttttcgcaaa
    12961 acaactcttc tgtaggaaat ctataatgta tctctgcttt tttcaatcca gctatgcatc
    13021 catataagtt ttttgtacca ccagtaaaag ttgccattat atgagtcttt aatttacaaa
    13081 gatttattat ataatctgaa tctactattg gtttaataat atccatatat tttagaacat
    13141 gtgctttttc actatgtatc ttaacatctg aaacatcata atttaactta atgcccagtt
    13201 catttgctac ttcttcaatt ccagatgcct tataaatgct cttaagagaa ctttgattat
    13261 atggaccacc agggctatct gctattgtaa tatcacaatt taactctaaa agtttttcac
    13321 atacaacttt tacaaccata ggatgtgtag ttgtagcttc ttcaggcttt tttttcatta
    13381 ataaatttgg ttttataagt actttgcttc cttttttaac aaacttatct ataccaccaa
    13441 tatcctctaa atttttttca atagataact ttacttcttc ataattgtaa gttttacaac
    13501 tcctaacaga taccgacttc atattatctc catccttcta tcacaacagt ttatttgtat
    13561 aatttaaaaa aggagaaacc aaagtttctc cttttaatct atgttttaat tattattttc
    13621 caaaatgtct gtttaataat cctaagaatg ctttaccatg tctagcttcg tctttacaca
    13681 tttcatgtac tgtatcatgt atagcatcta atcctaattc tttagctctc ttagctaatt
    13741 ttaattttcc atcagttgca ccatactcag catcaactct aactcttaag ttttcttttg
    13801 tatctgcaac tacaacttct ccaagaagtt ctgcaaattt agcagcatgt tcagcctctt
    13861 caaaagctat tctcttatac gcttcagcta cttctggata accttctcta tcagcttgtc
    13921 tactcattgc taaatacatt cctacttctg tacactcacc agtaaagttg gctcttaatc
    13981 cttcgattat ttctgcatct acaccttgag ctactcctat tctatgttca tcagcccaaa
    14041 ccatttcgcc tttcatttct tcaaatttat cagctccaac tttacatact ggacattgtg
    14101 ctggtgcagc atctccttca tgtatatatc cacatactgt acaaacaaat tttttcataa
    14161 ttaatttcct cccaatttta tataattaaa tttttatttt taagtttaat aacttgctac
    14221 aagaatatat ataccccaga ctttatagct taaacactaa aaataaaatt tatttcctaa
    14281 ttaattttat cactttgaaa caccagtatc aaccaagttt tgatgtcaaa tttctacata
    14341 atacataatt tatttgatta attaggaaat agttgtcata ttaaatactt tctaatgaag
    14401 actcctctga atctttaagt ttcacttttt tgctctttaa aaaagataat ttagtttctg
    14461 cagttatgat tgcaacaaat atcaagatac taccaataac caattttgct gtaataagct
    14521 cctttaaaat tattatagaa aatattgtac caaatacagc ctctgtggat aatattatag
    14581 ctgttctagt tccatcaact cttttttgac atatagtctg aaataaaaat ccaattgtag
    14641 tactaaatac agctagatac atagttccca tgtaaccaga taattcagct tccatcttta
    14701 attgacctgc aaatgtttga actacaacag acattaaaaa cgctactgta aattgaatag
    14761 ctgtcaatgc cattgggttg ttatcttttg caaactcact tgtaaagaat atatgaaatg
    14821 caaatccgaa tgaacatata agtgttagaa aatctccaaa attaatagag aaatcagctt
    14881 ctaaagaaag tatcccaatt cctataagag ctaccaaact acttattatt cctattttat
    14941 caagttttct tctataaagt ataaatccta tgaatggaac tattacaaca ttagctgctg
    15001 ttataaacgc atttttagat ggtgtagtgt acataagtcc aatagtttgt actgtgaatg
    15061 ctagaaataa aaatattcct aaaatccctc ctgcttttaa taatttttta cccatattag
    15121 cacgaatctg tttaaaaaac agtaaattca taattatagc tgccaagaaa aatcttaaag
    15181 ttatgatttg tagaggtgta agcccaccgt ctaatgcaaa ttgggttgct acaaagccac
    15241 ttccccatat aattgctata aatattagtc ctatttctcc aaagtatttt ttcaataaag
    15301 ccccctcctt tttatttttt tgcaaaatta taaaccccct aaacagcaag agagtttttc
    15361 tataagcatt actataaatt aatatagtaa attttttcag tattttaaat ctaaatttac
    15421 atacattttg agctcaaatt tatatccact atttaacagt aatatattaa tcattttttg
    15481 taaacacctt tttaataaat ttttatattt tttatatttt tttacctata ctaaattttt
    15541 ttatagatat ttttaatata tatgatactt taatattatg caaataatgt catataagtt
    15601 acactttagt tatcttagtt acaactttgt cggtttactt tttctacttt cttcgatata
    15661 attataatat atttttgata caattcgaca aaagggggaa tgctgtaatt atggtaaaaa
    15721 aaagaaaaaa gaaaactgtt acacttacta tacttgttgt aatagttatt ggtatttttt
    15781 ctatggcatg ggcggacatg tctagaaaaa aagaaatgaa agaaactcaa gaaagactta
    15841 aagctgaaag attagaaaaa gaaagaattg aaaaagaaaa aaatgaacct attattggtg
    15901 caaaaaaaga agctgtaaaa gaatatggat atgacgcaaa aatagtttgg gacaaactta
    15961 acaaatacga ctattcaaac aatggagaaa agatagtctt tcttacattt gatgatggac
    16021 catctactac taatactcca caagttctag atatcttgaa aagacatggt gttagaggaa
    16081 ctttctttat taagggtgat tctcttgaaa gaaaaggagc taatgaaatt ttaaaaagaa
    16141 catttgacga aggaaatgca atagcccatc actcttatac tcatgactat aaaaaactat
    16201 atcctaacag aagcctaaat ctcgatacat tcgtaaatga acttaacaaa actgatgaag
    16261 ctatgaaaaa agtcttagga aagaattttt cctcaaatgt agttagatgc ccaggcgggt
    16321 atatgtcatg gaaaaatatg gaacctctag gaaactactt aaaagagaaa aacatggcat
    16381 caattgattg gaacgcttta aatgctgatg cagaaggtaa aaagaaaaat gcacaagaat
    16441 tatttgaaca tgcaaagaaa tcttctgaag gcaaagaaat ggttgttctt ttaatgcatg
    16501 atacttatgg aaaacaagaa acagtaaatg cattggacca aattataaca tattttaaag
    16561 acaatggtta tcaatttaaa attttaatat aataaaaaag caagtttatt gaatgtacat
    16621 tttatactca atagacttgc ttttacttta ctctatttta ctagctcgat ttttacaaat
    16681 tttttctttc ctatttgaac aactaattca ctctctttta caatagtgct taaatcctct
    16741 actttttcac catttacctt tactccacct tgagaagcta atcttctaac ttcactctta
    16801 ctcttaacaa tctcattaga aactagtact tcgattaagt caaatccatc ttccttaact
    16861 tgaattgttt gtatatcttc aggtatttga cctttttgga atacagattt aaatctttct
    16921 tcagcttctt tagcagattc ttctccatgg tataatgtta ctatttctct tgctaagttc
    16981 atctttatat ctcttggatt tactgaaccc tctttcaact gtgattctat tttattaact
    17041 tcatcaggat gaacatctgt cactaaattg tagtatttaa ttataagttc atcaggaatt
    17101 tccatagatt tttggtacat tattcccgct tcttcatcta ttccaatata gttacctaaa
    17161 ctcttactca ttttttcttt accatctaat ccttctatta aaggcatcat tattactatt
    17221 tgagactcca taccaaattc tctttgtaat gatcttccca ttagtaaatt gaatctttgg
    17281 tcagttccac caagttctat atcagcttct aatgctatag agtcatatgc ttgcattaat
    17341 gggtagaaga actcatgtac tgatattggc atttgtcctt catatctctt tttaaaatct
    17401 tctctttcta acattctcgc aacagtaata gttgctgcta attttattac atcttcaaaa
    17461 tttaacttag ctaaccactc gctattaaat cttacaatag ttttttcttt atctaaaact
    17521 ttgaaaattt gttcttcata agtttttgca tttgcaagaa cttgctctgt tgttaaagct
    17581 tttctagctt tagattttcc agttgggtct ccaatttttc ctgtaaaatc tccaattatt
    17641 attactattt gatgacctaa atcttgtaat tgtttcatct ttctaagaac tactgtatgc
    17701 cctaaatgta tatctggagc acttgggtct agacccaatt ttactatcat aggctttcct
    17761 tctttttctg atttaataag cttttccctt aactctttct catctattaa atcatctaca
    17821 cctttcataa ttattcgcat ttgctcgtct atcgatttca tttttcattc ctcctaaaat
    17881 atttatataa tctataattt ttatttttat aataaaaaaa gctttcatcc tcttaaaagg
    17941 acgaaagcat tgcttacgtg ttaccacctt agttcatcaa tatctcacga tattaatctc
    18001 ttagagtaca attacatata ctcgtgtact ataacgtgta ctaatccgat atctcttaat
    18061 ggttgttatc tttcagagat agtgctctga gatgtattca aactaaatca atttattcca
    18121 tttcaccaaa ctggaactct ctgttaaata aaatctagcc ctacttcttc tcttcatagc
    18181 atttatatat tcagattatt tcatcaatta caagaaattt ttcttaactt actcataata
    18241 tattattatt tttatatttt gtcaaccttt tttattatta tatacatgtt tttttacatt
    18301 atactacttt tttatatttt ttaccaatac ccaaaattta tttttcaatc aaaagattta
    18361 tttgtttatt gactatatta ctctgactca atcttatatt tattttctta tttaaaaatg
    18421 cgtttataat tattaaacca ataactgtta catcaaatat aaaaaacagc aataaaatta
    18481 ttatcaaaaa taaaattcta gcatataata attttctaaa cttagctatt ataaagtttt
    18541 gtagtgacat acatgagtct ctaatgataa aatagagact tatataggtt attgatataa
    18601 agattgtttt taaataaaca gaatcaactt taacattcaa cagttttaga acttcaaaaa
    18661 tggctagata agaaacacat atgtaaaaag ctttttttga gcaacataaa aattggttta
    18721 aacttctaaa attcttaaat attaaaaatt tttttagaga attagaatct aaaatatgca
    18781 atttattccc taaaattaaa cttaataaat atatgcaaaa caccatacca aaaggaaaca
    18841 atgctatcat taaataataa ataaaatttg tatacccatt aaaataaatc catttgctat
    18901 atccttgaaa ttctttcata aaactataac ctattaatgt tgaagcatat atatcaataa
    18961 aaaccattac aataagccca aatactgaac catatactaa atcatccata accattgtag
    19021 gcttatttat tagatagcca cacattatac caagtaatac actctgactt gcccaaatag
    19081 cagttcctag atttccaagt accagagaaa ttattaaaag cgaagtaatt ccagataaaa
    19141 tggtatactt taaatcacat ttcaagcaga ttatacaaaa aaatactggc actataaaat
    19201 ctaagtaaat agcatatcca aatccagtac tcacagctat aattgttact actatgaata
    19261 aagatgacaa taaagctgct tcagttaatt ttttagtagt attcaaaaca ctgcctccat
    19321 aaatcataaa ttttctaact aaagttttgt ttttaatata gtatatcact ttttaaaaat
    19381 atttttaata aatataaatt ttaataaagg tatttatgtt aaaaatggta taatgtatct
    19441 taatattcag ttttttaaga tgctaggagg taattactat gtttaaaatt aaacacttta
    19501 atgatttgag tttagatgaa ttttatgaaa tagcaaaatc aagatatgag gtatttgctt
    19561 gtgaacagaa gatattttca ttaaacgatt atgatgatat agataaaaac tcttatcaca
    19621 tatttttaaa agaaaatgga ttgatttgtg cttacgctag aataattcct aaagaataca
    19681 gctcttataa tgatgtttcc attggtagag ttttagttct ttctagtcat agaagaaaag
    19741 gattagcaaa acaaatgatg gattgtgcta tagattttat aaaagttaac ttacacgaaa
    19801 ataatataac cttatcagca caaacttata taaagaacct ttacttatct tgtggtttta
    19861 aagaaatctc agaagtatac gacgaagcag gtattgaaca tattaaaatg agattatagt
    19921 gttgacaagc taatattgtt aatatataat ttaagtataa attaaatatt gaattgatat
    19981 ttaggtattt gaaaatacct tacatagagt cagaaggctt ttgctttctg acttttttat
    20041 tatcaaaatt caataattat atggattcaa agtgttaatt tttattctat attctacttt
    20101 ccccaaaagt aagaattata cccaactttg tatgataggc atcttgccta tctttttttt
    20161 gtaaaagtaa caaggtaata atataatgca tagtttaaaa taagaagatt agcccaggct
    20221 aaatttcaag catggaggtg ctattatgaa aaaccttaac gatattcaag tgaaaaaaac
    20281 tgtcaaagtt gaagatatat tatctagtgg taatttaaga gaaagaatgt tagcgcttgg
    20341 tcttactaga ggagctgtta tagatgtagt tagaaaaggt ccaaaaaata atttaactgt
    20401 ctataaaatc agaggtagca agatagctct acgacaagaa gaaagtagct taatattagt
    20461 ttctgacatt taaaaatttc tagcaggagg atattaatat ggggttgaca cataactcaa
    20521 ctaaaatgag ttctttaaaa gacatgtttg atatagacaa taaggaagac cagtttgtaa
    20581 ttgcattggc tggcaatcca aatacaggta aaagtactgt atttaatcat cttacaggac
    20641 ttagacagca tactggaaac tggccaggaa aaactgtagc tacagctcgt ggtaatttta
    20701 aatataaaaa taccgaatat gcattaatag atttaccagg aacatattct ttatttgcct
    20761 tatcacaaga agaaattgta gctcgtgatt ttatatgttt tggtaatcca gatgcagtaa
    20821 tagtagtatg tgatgctaca tgtcttgaaa gaaatttaaa tttagtattt caagtgatgg
    20881 aacttacaga taaagttatt ttgtgtatta atttaataga tgaagctaga aaaaaaggca
    20941 ttactattga taaaaaactt ttagaggatt ctcttggtat tccagttatt ttaactgctg
    21001 caagaaatgg ctctggtatg gatgaactat tggatacttt aaatgatgta tcttttgaca
    21061 aatataaatt aaataataaa cctgttagat acaatgaaaa tatagagaat gtggtaaaat
    21121 caattcaacc agaattggat aatattatac caggtataaa ttctagatgg ttgggactta
    21181 gacttattga tggcgatgaa agtatatttg aatctatgag taattatata gacaaaaatt
    21241 ctatagatgc tattaatgaa gtaaagaaaa agattccaga caatataaat aaacagaaaa
    21301 taagagatga gtttactaaa atcaactatg attatgctaa aaaactgagt gatgagtgtt
    21361 gttctaatgt tgctaaaaaa tcaactgata gagaagaaaa agtagataaa atattaacat
    21421 ctaaaatatt tgggcttcca ataatgctct tattattagg tactatattg tggattacta
    21481 tagaaggagc taactatcca tccactttat tatctaattt attactcgga tttgagccaa
    21541 gtatttcggg tatattaaat agtattaatt gtccatcatg gttaaatgat atgttggttt
    21601 taggattata cagaacatta gcatgggtaa tctctgtaat gctacctcct atggcaatat
    21661 tttttccatt atttacccta cttgaggatt ttggatattt gccaagagtt gcatttaact
    21721 tagaccacct atttaaaaaa gcttgtgcac atggaaaaca gtgtttgact atgtgtatgg
    21781 gatttggatg taatgcagct ggagttattg gttgtaggat aattgactct ccaagggaaa
    21841 gacttatagc tatacttaca aacaactttg ttccttgtaa tggtcgtttt cctactttga
    21901 tagctatatc tacaatattt tttagctcag ttataaccaa ctctttcgta tcaagcgtag
    21961 caactgcact ttgcataact ttactgataa ttctaggtgt tataataaca ttgttggttt
    22021 cttacactct ttctaagacg ctattaaaag gagttccgtc tacatttaca ttagaacttc
    22081 ctccttatag agttcctcaa ataggaagaa ctttatatac ctctataata gatagaacaa
    22141 tatttgtttt aggaagggct gtaatggtag ccatacctgc tggtgtcata acttggatat
    22201 ttgcaaatat atatatagga gatttaagta tattatcaca tgttgcaaac tttttagacc
    22261 ctttagctaa attaattggt cttgatggtt ttatattact tgcttttatt cttggattcc
    22321 cagcaaatga gatagttgta cctatacttt taatggctta cctagcaact ggttctatga
    22381 tagaattaga tagctttagt gctttaggtc aagttcttag agaacatgga tggacttatt
    22441 tgacagcttt aaatgttatg ctgttcagtt tactgcattg gccttgtgca acaacacttt
    22501 taacaataaa aaaagaaact ggtagtttaa aatggactgc cttaggattc ttaatgccta
    22561 ctatacttgc ttttgttgtt tgttttttaa caactacagt ttacaattta tttatataat
    22621 tagtattaac tgacagtaga catatattag agaaaccatt taaaaattta tatactttat
    22681 tataaagcat agagatatat tcaatgataa gtaaaattac ttaacatttg aatatatctc
    22741 tattttaatt cttatctgga tacttggatt gaaatttcag tcatataatt atcttcatta
    22801 ctttcacaaa aaaagtcaag tatactctcc tcatacgaat ttccaacaat tttatattca
    22861 ttttcatata tatatttttt caatttttcg taagatttat atgaatcctc atattttcca
    22921 atatgtttta tgcaagcata cattccttct ggctttacat atagcttttt atcctttatt
    22981 tttttatcta tcttcaaata aaaacttgat atacttaaat agtccccatt ttttatattt
    23041 tcattactta ctattactcc caactcatat ccttgataca aatttttcga tttacaataa
    23101 ttggtaaaat ctattaattc catcataaaa tcactattac tcatattact caaatcgctt
    23161 gatagaacta aatattcttc aaactttttg gatatataaa tttcttcact attcatttta
    23221 gaaattcctt caactgttat atttattcta ttttgcatct tagaacttat ctttcttagt
    23281 ttttttattt tttctcttat ctttttttct tcttctttaa gcattttcac acaaagctgg
    23341 ggagttctat tttgtatata ctcttttatt tcctctaaac tcattcctat ttctcttaat
    23401 gattgtaata tatcaagtgt atcatattga taaactgagt aatatctata attattttta
    23461 tctttatatt caggagaaaa aatacctatt ttgtcataga aaattaatgt ctgcttagat
    23521 ataccacaca atttagcaaa ttcacctgtt gtaaaatact gtttttccaa gggttataca
    23581 ctccttttct ttttgttgtc tatatagtta ctatattctt tattatttac taaatcaagt
    23641 agttattatt ttagaaaatc aatatggttt tgattttaaa gcttaaatat cagttagatg
    23701 tcagttataa atattttacc ctttcccatc ataaaaaaga tatcccctct taacttttgg
    23761 atggaatatt atatttatac ttgttagagt gaaaattgta ataggtgcta caataagata
    23821 tttaattaaa cataaagtta attattgtaa taaaaaatct ttagataaat tagagttaaa
    23881 gtacggaaat atagatagag aaaaagctgt taaactagaa atattttatc agtatttaat
    23941 agaacttgaa tatatagaaa tgagtttatt aataagaaga ttagatactg caataacatc
    24001 attagcatta gtatctatta taacaatagc atcatattat ttaatcagaa aaaatgtgta
    24061 attatgtaag ctatttataa taaaacactg ataaaattaa taataaaaag gtaataggat
    24121 ttctatacta cctttttgtt tttataatat agtgtaattt tattatattt ttgtatgcta
    24181 atgtaactag ataatcataa gataacacat taaaagaaaa ttatgaaaac ttacattctg
    24241 tatagttaat ataaaacata agtaatataa atttatgtaa agcaacaaaa gatagattta
    24301 cattccatat agttaatata aaaccttaga gaaagcgaaa acgtaccatt aaaatcaaat
    24361 atttacattc catatagtta atataaaacg ttgtaatcat agttccaatt agccacttta
    24421 acatgcattt acattccata tagttaatat aaaacaccaa aataaactta gtattttcaa
    24481 cacttacacg tatacaactc tcctaaattt gcagtgaacg agcaatagtg caattgataa
    24541 cgcttatcat gcactctcaa cgccttatat ttcaactgtt acgccaaatt ttttcacaaa
    24601 tatcgctcac tgcaaaattc ctataatttt attatatcat aaatatatta cttttgaata
    24661 ttgataccaa tttataccat aataaaagca aaaaaacgta atctatctac attagattta
    24721 aattctataa cttaaagttt tttacataat atttatgctt gtttaatcaa tctaatatat
    24781 cattcttgta tattgctttt aatagtcttt tttcttaaat aaatatctat ctgaacaagc
    24841 atagtttaat taaaaacttt tgtattccct taaaactgta tctacctctt attattttat
    24901 ttatgatata ttatttttaa tctaaaaata gatatctatt tttattatgg ttatcccttt
    24961 ttaaaatact ttaatcatat actattccca atttaacatc cattgaaatg ctattttctt
    25021 atattgcaat ttataattga tatatcttca atataataat ttaatgtatt tttgatttta
    25081 aagcttttca gtgatattat tgataaatta gaattaatta tctagcttta aaaggtcaaa
    25141 gatttctgtt gctgtctgcg gttttccaaa taaatatcct tgtatgtaat ctggctttat
    25201 ttgtttaact aaatcatact cttcttgtgt ttctacacct tctaaacata cttttattcc
    25261 tacatcatga caaattgcaa caataaaatg tataaatgta gcatcaaact tactctttaa
    25321 tatatcttta acaaatactc tatcaatttt cactatatct attggagaaa attttaaaac
    25381 ttctagagac gagtatccag taccaaaatc atccatagca atataaattc ctaaatcttg
    25441 taaagcctta aatttaaact gtaaaatact catattttgt actgtatgac tttctgtcaa
    25501 ttctaaaaca agattttgat aaggaaaatt ttcatcatct ataattttta ctatatcttc
    25561 aataaatgtg tcttctaata tttgaacagc tgaaacgttt acactcactt taaaaaaagg
    25621 atagtaatca atccatttcc taaaagtacg taatgccatt cgaagcaccc aagcccctac
    25681 aatatttatc atatcatttt cttctaatat tggaataaat tccaaaggag aaattgcttt
    25741 gcatttgtca ttagtccacc ttaaaagaac ttctacacct attattttat gactttctgt
    25801 atctacttgt ggctggaatc gaagactaaa ccccctaaaa tcatcattaa ttgatgcctt
    25861 taaatcccgc atcatttcta atgaatacat tttatttttt aatatttctt gggagaaaaa
    25921 aacaagcctg tttttaccat gctctttcgc atattgtaaa gagtaactag cacacttata
    25981 aagttctttt actgtatctc catgttttgg atatattaca caaccagcag aaattgttat
    26041 atttaatcca tattttctcc atagttgaag gtgtataatc atattctgta tttgattata
    26101 tagagttaga atttcatttt cttcaacatt atcaacaaga atgcccattt cgtccccatc
    26161 aagtttataa agttcagcat tatctggtaa aatagattgt acagactgtg ctagtgtttt
    26221 tattatatta tctccaaaat ccctatcata catctcatta acatttttaa aatcgtcaat
    26281 atctaataat actatgcata atttttcaat cataggattt gaaattttat cttcaaatac
    26341 actcataaat tcatgataat tcagtaactg tgtcaatgaa tcaatcttat tttgtttacc
    26401 taattgcgtc attattcctg caaaaatact tggctctcca aattcatcac gcattagttg
    26461 acctctacat cttaaccata tatattctcc actacgattt ttagccctaa attccactgt
    26521 atgataatcc atctggtttt tacctatttc agtgtttgac ttataaaaac gattccagtc
    26581 ttctggatgt acaatttttt tccagtaaac aagcggattt ttaacaattt ctcctggtaa
    26641 gtcaaataat tcaacttgtg atggtgaata tcgaaatacg cctgttttca tattgcaaat
    26701 gtatatatat tcatctgtac ctctaatcaa agcatcgtaa agcaatgtag aattataatc
    26761 aaaattgtct cttgaaaaat taaaaactcc tttttcttct ttattatttg atatttcatg
    26821 aaattcatta atcttaattg gtttatttaa gcttgttgag tttgcaatat ctattgattg
    26881 atgcatatga acaactgtgc catcttgcca tgtaattaaa ctatcataat tttcaaaaac
    26941 acgatttaac ttattacatt tttcatacca ctttattaat actccttttt tatcattctt
    27001 taataattct aaaactttac aaaaactaca agtagaattt ttttcaggat ataatacttg
    27061 ccaacaaact tttccttctg gatctaaaat attatattct tctttcattt ttttgttcat
    27121 aaaaataatt tcattagtat gaatatttgt aacatatata ttaatctgta gttgatttaa
    27181 tataacttca aaattatgtt tgttcatata cttatctcct ccatagttac tgctctttat
    27241 aacaacattg ttaaaattat tctttttaga agatttactt ataacttata catatacact
    27301 aaatacctta atttaattag aaagtttatt acttcatatt ttaaattata attgataaaa
    27361 aaaaatacag caatatagta ttaccaattt aaaaaaaatt aatattacat aatacatctc
    27421 tctcttatat taaattttaa cttggtacta atcttgacaa attgt
//
LOCUS       DAYAPA010000048        25973 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.048, whole genome shotgun sequence.
ACCESSION   DAYAPA010000048 DAYAPA010000000
VERSION     DAYAPA010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25973
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.048"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(183..1775)
                     /gene="hcp"
                     /locus_tag="ACGI59_001861"
                     /gene_synonym="priS"
     CDS             complement(183..1775)
                     /gene="hcp"
                     /locus_tag="ACGI59_001861"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HFG9739647.1"
                     /translation="MEKMFCFQCEQTAGGKGCTINGVCGKKGGTANLQDELTGVLIGL
                     SRATIGNKNRPTSETDKIMIEGLFTTITNVSFDDEAIKRQIEKTEVEKSKLVPRCSDC
                     STTCNRNDNYDMKDLWNDNEDIRSLKSLILFGLRGMAAYAYHAMVLGYTDKDVNEFFY
                     EGLIAVGGDLSIDELLGLVMKTGEINLKCMELLDRANTETYGTPEPTQVSMKIEKGPF
                     IVVTGHDLYDLHKLLEQTKDKGINIYTHGELLPAHAYPKLKEYKHLKGNFGTAWQNQQ
                     KEFDNIPAPILFTTNCLMPVKDSYRDRVFTTEVVAYPGMVHIDEDKDFSSVIEKALEL
                     GGYREDVHMTGINGGDTLTTGFARGTVSSVQDKVVDAVKSGAIRHFLLVGGCDGAKPG
                     RNYYTEFVKQAPKDTVILTLACGKYRFNDLDLGDIGGLPRLMDMGQCNDAYSAIKVAV
                     SLANAFECGVNDLPLSMVLSWYEQKAVCILLTLLHLGIKNIYIGPSLPAFLSKNVLDI
                     LVDKFALTPISTPEEDMKKILG"
     gene            complement(1835..2560)
                     /locus_tag="ACGI59_001862"
     CDS             complement(1835..2560)
                     /locus_tag="ACGI59_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFG9739648.1"
                     /translation="MMRKIIKIDEEKCNGCGLCVEACHEEAIGMVNGKAKLLRDDYCD
                     GLGDCLPTCPTNAISFEYREAAEYDEAAVKANMEAKKAQKKTLACGCPGSQSKSINRE
                     VSNSTSISNDIVEIKGSQLNQWPVQIKLVPTNASYLKNASFLIAADCTAYAYGNFHNK
                     FMKNKVTLIGCPKLDEVDYAEKLTEILKENDIKNIVVTRMEVPCCGGIVNAVKTALQN
                     SGKMIPWQIVTISVDGKIVDGEI"
     gene            2725..3441
                     /locus_tag="ACGI59_001863"
     CDS             2725..3441
                     /locus_tag="ACGI59_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFG9739649.1"
                     /translation="MQQGSDYLKKFLEKHIDLLKKIPLFEGIKSDELEEMLECLGVVD
                     KTYPKDSIIFSVGSEVTSIGIMLKGSAHIIKEDIEGNRNIVAELLPGDLFGEVFACTR
                     LHKSNVTVTTTSSCEVLFIKFKSVTGICSSACVFHNRLVENMLQLIAEKNILLHNKIE
                     LLSRKTTREKLLMYFSKQIEQTGSHQFTIPFSRNEMADFLCVDRSSMSRELGKMRDEG
                     LLSFNKNKFEIFYFQNKLDI"
     gene            complement(3566..4141)
                     /locus_tag="ACGI59_001864"
     CDS             complement(3566..4141)
                     /locus_tag="ACGI59_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HFG9739650.1"
                     /translation="MVKVLAFMGSPRKKGNVDTILDEIIKGVNDNNVEVKKYYLNDMN
                     IKGCQGCLYCRKVPTCAFKDDMIDIYEDIKSAEYIIIGSPVYICQVSAQTKLLLDRLY
                     PLTEINKSKHIPRFGQKKLIMVYTQAAPISFLFKKYFKYTAKHLKGMGLNHYKTIVVT
                     KAFERNSTKKNFKVLKKAYKLGMKIAIDING"
     gene            complement(4315..4737)
                     /locus_tag="ACGI59_001865"
     CDS             complement(4315..4737)
                     /locus_tag="ACGI59_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFG9739651.1"
                     /translation="MKSLFKFNKELCTACGACLLACIDQNDNNIDNGELFRHVFTIEE
                     GENIEYFSLACIHCNEPKCLGVCPKNCFKKEEGFIVLDNQNCIGCKLCEKACEYGALI
                     YGNDGKANKCNGCIERYKCGMDSPCVMVCPTGALKFNK"
     gene            complement(4751..6928)
                     /locus_tag="ACGI59_001866"
     CDS             complement(4751..6928)
                     /locus_tag="ACGI59_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HFG9739652.1"
                     /translation="MYNKYNNLYLLLGKENVNMDSYKQLKAKIPCKETGIEIKRTLCD
                     ICSPGVHCGIDAYVKDGKVIKIEGTKEHPMNKGLLCTKGLCNREYIYREDRIKTPLKR
                     IGKRGEGKFKPISWDEAYREIAEKLNRVKSEYGAKHVAFFTGYGKWYRPFLQRFASSF
                     GSPNYGTESSSCSTSAQMAGNVATGRNFTMQDMENAKTYLGWGFNPYYSKFLNSVNLI
                     KLKEKGLKFIIVDPRITPTTQKLADLHLRIKPGTDGALALGMANLFIQKDLIDREYIE
                     KHVHGFNEYKEYVKLFDLDTVSRITGVSKDNIVKAVEMLSQGPFCINESAAPIVHHQN
                     GFQNYRAMMSLSAITGNYDRIGGNIPKQDYFAGKIGGFQTLEKEFVKEVLPKNINERI
                     GGERFPLWNEMVTQFQAMDLPRQIEEKTPYEIRALFCHGINARMFPNSGRLFKALNSV
                     DFFVNIDLFMTDTSKYADILLPACTSFEREECKSYPGGYITYTKKVIDKLYDSKSDVE
                     ILSDLANAMNIDDDLLKAGYEASVRHMFKNTCVDVDKLKESDLPLKCKDFKPYIVGSY
                     TREGYDTSTSRFELKSTIIEKYKDFGLDALPTYRGFNDNIDDEYTHLLTSGARISNAL
                     HSRLHDIKLSRILKNEANCDISSLDAKELGVISGDYIKITTKIGSLDVKVNVTDRVLY
                     KNIHMYHGYREADINSIICDNFDPYSGFPAYRSCKCKIEKMNR"
     gene            7209..10388
                     /locus_tag="ACGI59_001867"
     CDS             7209..10388
                     /locus_tag="ACGI59_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HFG9739653.1"
                     /translation="MKIDMIYKLIRRNTEPSRWSRGVKYYTNQLVDYIDIDIDDLQNN
                     LLTFRGGVNSEDNYSFYFNSITIDIKHFKIIENDCSCADCQSKGKSIFICKHIVAIVI
                     KSLKQIKNKKDVPPILIDILGADKDKLSNTNSKTSLDLSKQLLSLLDNTKRERVNLDV
                     LINVDSNKAIAEFKIGINKMYILKNITEFAYSKLNGYELDFGKKFTYNPNTHYFADED
                     EKLVDIIEEYGTNLSYNFSSSHSKKFMEINASSLKQFLNAIKYKDFTLIFSKEEYHPK
                     ILNDTLPVRFDFKNIEDKIVLYSKDSLPVPLTKKNDVLLYDGNIYLLSHKKAHDYSKI
                     YQILSKTKELKFDKEDSKDVLGLLVPRLKSISQEVHLDDNIKNNITKDFKSEFYFDMI
                     DGNICCDVKYIYDDEDKKFVLPNIQKEATIENKLTSSQFTKENNHYVFKGTDHDLFTF
                     LDLQLESLKEFGDIYYSEKFKLKKIYNASSIQASINQTNQNYLEFNFNISDINPKEYK
                     DILKAFQEKRTFYKLKDGNFIDLSERETKDFFELVENLDIMDKSKNNKIHKNKALYIN
                     DVIRSKNLKFIDGKKELDSICEKFRNFDSINLEIPANLNANLRDYQINGLNWFKVLDY
                     YKFGGILADEMGLGKTIQTIAFLLSLSNKKSLIVTPTSLIYNWKNEFEKFAPDIKVLL
                     IHGNKRDREKCFMELENFDVILTTYGTLRNDLDKYSEIKFDYCILDEAQNIKNPVALV
                     TESVKSINAENKFALTGTPMENNLLELWSIFDFIMPGYLYSKAKFQELFINKEDNVKN
                     LKKLIKPFILRRSKKQVMKELPDKIEKNFFVELNKEQKKIYSVYSKDIQDKMKDKNLK
                     KDKIVIFSYLTKLRQLCLDPSIVVKDYNKKSSKIETCLEILRDSINENHKILLFSQFT
                     SVLKNISKELDKYKIKYHYIDGKTNAKERLELVDEFNNSMDKKVFLISLKAGGTGLNL
                     TSADMVIHFDPWWNPSVENQASDRAHRFGQKNSVQVIKLIAKGTIEEKIIKLQESKKE
                     LINQFINGELSNEGVLKSLSDEEIINLFN"
     gene            complement(10673..11437)
                     /locus_tag="ACGI59_001868"
     CDS             complement(10673..11437)
                     /locus_tag="ACGI59_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFG9739654.1"
                     /translation="MAYFNYNDKQCYYNEIGDGTPLLFLHGNTASSKMFNDIINFYKD
                     EYKVILIDFVGHGKSQMVDKFSADLWFDEAMQVICFLEAMNYKKVNIIGSSGGALVAL
                     NVALERPDLVNKVIADSFEGEVPLESFVQNVKIEREASKQDDGAKAFYIYNQGENWEK
                     VVDNDTEAIFEHYKTIGKFFHKPLETMQPEVLLTGSREDEFVSLISSDFFENTFSLLL
                     EKIKNGKMYLFDKGGHPAILSNGLDFSNVAKKFLEE"
     gene            complement(11593..11826)
                     /locus_tag="ACGI59_001869"
     CDS             complement(11593..11826)
                     /locus_tag="ACGI59_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9739655.1"
                     /translation="MKAEDMKRKVSPIDLERILVTGGVIKHEDSIWLLDFWGNKDIAG
                     ILLMPPTRHVILHLNDCCKWKEYFRTKKKFYRS"
     gene            complement(11900..13393)
                     /locus_tag="ACGI59_001870"
     CDS             complement(11900..13393)
                     /locus_tag="ACGI59_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFG9739656.1"
                     /translation="MKYTFEKFKLNEKILKSLKSLGYNIPSRVQREVIPKLLKGQNLV
                     VRSKTGSGKTASFAIPLCENINVDYNNIQALIVVPTRELALQVKDEISDIGRLKKVRC
                     SAIFGKQSIKDQIAELKQRVHIVVATPGRILDHINRGSIKLENVKYLVIDEADKMFNK
                     GFVEQMEKILLNLPKEKIVSLFSATIDEEIKYICEKYMLDYSVINIEENESDTNQKTR
                     QIDDKIIKANGREKYILLKELIYSENPKSVIIFCNTKEKVSKLYNKMSKEGFLIRELH
                     ADLSQERRIFVIKDFKNQKFNILVSSDVASRGIHIDDISLVINYDVPQDKENYIHRIG
                     RTGRKGNSGKAITIVTEKDEKYIENIETYIGYKINELKDIEKSRITHGKVLFEEYSKG
                     ILKKVSKRKKVDKNSYKSKDIKLNIESEVVKLYLNAGKKKKIRVLDIVGAFSNIKGIT
                     NDDIGVIEVQDLCSYVDILNYKGDLILKKYKEIPIKKKMVKVKRDIR"
     gene            complement(13721..14263)
                     /locus_tag="ACGI59_001871"
     CDS             complement(13721..14263)
                     /locus_tag="ACGI59_001871"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFG9739657.1"
                     /translation="MKICITVGHSILKGGKSTGVNGIVDEYNYNKKLAPMLAEMLISQ
                     GNTVDVIICPERHFMSEREEFFYRVPKVNSGKYDLLVELHLNKADGTQCGTEVLYYGT
                     EGLEYAKRVSNRLGELFENRGAKKRENLYILRNTNPVAIQIESFFCDNVNDCNKANES
                     GYDYIARLITEGILNKDINM"
     gene            complement(14553..15500)
                     /locus_tag="ACGI59_001872"
     CDS             complement(14553..15500)
                     /locus_tag="ACGI59_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9739658.1"
                     /translation="MRITKTTFNDVFSNDANGSIIKVEKYVDKKCASVFEILTYTIIV
                     TNTSRYKTGNIFFKDYISKYIEFINNTVKVNGIIKRGLDPQQGFYIGRIDASCKKIIS
                     FKSVVLPNSAHRIIKNSANIYYYYKCNLDKFPTRISIESNRVYTNVNKIVFKQLNISN
                     TLKAPKNLRDILKVNVNSKIFDVKPMKNLINKGSNLKLCNLIVFGYIEVEIDYSCKNR
                     SNFKTVNKSNDIKKDKYYKNDIQNNNENINDCKKQISFGDNKVKKITKTFGFSCFICS
                     PVGIIYEDMKNINIKIEHTSINELNPEELFVNTSLLLYY"
     gene            complement(15528..16019)
                     /locus_tag="ACGI59_001873"
     CDS             complement(15528..16019)
                     /locus_tag="ACGI59_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9739659.1"
                     /translation="MYENNKVIYNLERCEKLEKTLYLSQFNTYKNFKLASKKTVINSL
                     ETYVYNMNINNISLVDTIECISLEDEQLTGKKLILVGDIDIDAILDYAGNKRNRNSSK
                     KNTFKLKIPFSTFIQMPRKIENKDKINLKYLIQDITSSILDDNLFISVTAIISYENSS
                     KIE"
     gene            complement(16462..17355)
                     /locus_tag="ACGI59_001874"
     CDS             complement(16462..17355)
                     /locus_tag="ACGI59_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFG9739660.1"
                     /translation="MKNKKFIVIILIISILIGVLVKEYSSREIKKDDINVSKYIKYAD
                     LASKNNAQVNWKYVASIVAVLNKNNLKNVKDSQIQEVSDLFVKNFSKNNKINKLSDIL
                     DELEFSNRQKRLVDNYIDNLKDYGIKPERLKSDTKYMKFIDEIKTEAIQNYKDYKILP
                     SITIAQAIIESSWGKSTLAKQYNNLFGIKADAYWKGKSVTLETKEHLDTIIDDKFRIY
                     DDKNESIKDHAKFLATNKRYKNNGVFDAKTYIYQAKALEKAGYSTAKDENGNSIYAAR
                     LIELIQQYNLQLIDSEIQSEM"
     gene            complement(17580..19865)
                     /locus_tag="ACGI59_001875"
     CDS             complement(17580..19865)
                     /locus_tag="ACGI59_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HFG9739661.1"
                     /translation="MKCLFSNVDEWKIIQDKIEIKENRLAESIMSIGNGYMGMRGNYE
                     ENYSGDTHKGSYIAGVWFPDKTRVGWWKNGYPEYFGKIPNSVNYIGVRIFIDDVELDL
                     AKCKVEDFHRELDMKNGILNRRFIVNINDKKFEVRVTRFLSISVKELAVIKYSIKSLN
                     SNTKIKFVPYLDSNIKNEDSNYEEKFWNGISSSSLEYKGSILSRTKENAFNTPVFTVG
                     SMMYIKTNGDVEAVSHESDYNYCENSITMDVLKNEVASIEKYVSITSTRDYKESELLR
                     KCEDILNRELSKSYDDILNEHIKLWNKRWESADVKINGDKSSQQGIRYNLFQLFSTYY
                     GEDSRLNIGPKGFTGEKYGGATYWDTEAFCVPVYLGVADKEVTRNLLEYRYNQLEQAK
                     DNAKKLGLKGALYPMVTFDGVECHNEWEITFEEIHRNGSIVYAIYNYTNYTGDYKYIK
                     EKGIDVIIEIARFWASRVHLSTKKDLYMIHGVTGPNEYENNINNNWYTNYIAKWCLDY
                     AVENILRLEKECNECIDRNNVTREEVEFWKIISNKMYLLYDEELQIIIQNDNFLDKEF
                     VEVKDLPKENFPLNQNWSWDKILRSCFIKQADVLQGIYYFGNRFTKEEKKRNFDFYEK
                     YTVHESSLSSSIYSIIASEIDNLEKAYELYSRTARLDLDNYNNDTNDGLHITSMSGSW
                     LSIVHGFAGMRTWNETLSFDPKLPKEWKNYSFNINYRDNIINVDVCSDLIKIENLKGK
                     PIKIIVYGKKYILEKCIKIDK"
     gene            complement(19862..20542)
                     /gene="pgmB"
                     /locus_tag="ACGI59_001876"
     CDS             complement(19862..20542)
                     /gene="pgmB"
                     /locus_tag="ACGI59_001876"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFG9739662.1"
                     /translation="MIEAFIFDLDGVITDTAYYHYMAWRKLAHKVGIDIDTKFNESLK
                     GISRMESLDRILEFGNKKYSFSEEEKVRMAEEKNNYYVSLIDEITSNDILPGIESLLI
                     DVKSNNIKIGLSSASKNAINVLNHLGISDKFDFIADAGKCKNNKPHPEIFLMSAKGLN
                     VNPQNCIGIEDASAGIDAINSANMFSVGVGNYENLKKANLVVDSTNQLKFEYIQEKYN
                     EYIVRRII"
     gene            complement(20766..22649)
                     /locus_tag="ACGI59_001877"
     CDS             complement(20766..22649)
                     /locus_tag="ACGI59_001877"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HFG9739663.1"
                     /translation="MSNKNKENITLSNQIKGTIQNSLTKEAILHIPMSNYAYGYDRET
                     LHIRLRTKKNEAKRVILRIGDQYVWDKGGAGGGNLNASGVGWSGGTNIVMSKEVETEL
                     FDYWIAKCKPLNKRSRYGFIIEGQEEKLLFTEKRIIELGNENDEKELCEIGNFFGYPY
                     LNYIDIPKVPNWVKDTVWYQIFPDRFANGDPSINPEGVDKWGAIPTRDNFTGGDLQGV
                     IEHLDYLSDLGINGIYFCPITIGKTNHRYDTVDYMEVDPTLGDKETLKKLIEEAHKRN
                     IKIMLDAVFNHIGYYSKQWQDVVQNKENSRYKNWFYIKDMSKIDTPIEQIDEKNIPYE
                     TFGCEKYMPKLNTENSEVIKYLLDVGKYWIQEFDIDAWRLDVSNEVDHVFWRKFREEV
                     KKVKPDIYILGEIWHGSLPWLMGDQFDSVMNYLMSEAMKKFFCTDEINAEEFKYMIND
                     VMVSYPIQVSEVIFNLLGSHDTTRILTYANGNIDRFKLAYMFMFVQTGCPCIYYGDEI
                     GMEGELSSISEGQRKCMEWNEEKWNKDIFDFMKKIIRLRKENKELRSISNEWVLADKE
                     NGTIILKKESISIIINNSSKNIILTLPDYLANKKVKDLYEEEIVDLKEELMLEKYKFI
                     ILK"
     gene            complement(22907..23728)
                     /locus_tag="ACGI59_001878"
     CDS             complement(22907..23728)
                     /locus_tag="ACGI59_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HFG9739664.1"
                     /translation="MNLIDNHIHTNFSSDGKDSMEDTIKKAISIGVRYLTFTDHLEHD
                     EDRGFCINYNDYVPVFNKFKEKYKKDIELLLGVEVGYRKHLKNEIEEIINSNPFDFVL
                     CSTHTIDNVPVPSKAYFKGLSKEDAYYKYFNSILETNREFGDYNIYGHLDYISRYGIY
                     SDNRVIYNDFKDVIDEVLKSIINNGSGIELNTSGYRYGLNAIHPNEDILKRYKELGGF
                     IVTVGSDSHRVEDICKDFDVAYDMLKYLDFKYVSLFKERETYFVNIEKVKSNNIA"
     gene            complement(23919..24272)
                     /locus_tag="ACGI59_001879"
     CDS             complement(23919..24272)
                     /locus_tag="ACGI59_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9739665.1"
                     /translation="MKKSNIFSIIFPTVIMVLMFLTFATDIFNIPDIQSKSIFVVGMV
                     LIFPISFLIQGIICVLNKTNWILSLIVSLVTYIVLMYIFLNDSAYIYVLLYTAFYMIG
                     HITAKLYYKIKTFKN"
     gene            complement(24314..25756)
                     /locus_tag="ACGI59_001880"
     CDS             complement(24314..25756)
                     /locus_tag="ACGI59_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HFG9739666.1"
                     /translation="MRTIRRVLALGLTLAIFLINVPNVDALTSDTIKGNNIYETAGLI
                     ADKKSYDTAIMVNMDNSIADGLSASGLAGAVDAPILLAQKNKIPNETKQRLKNVKKIY
                     IIGKELSISKSVETELKNTGAQVTRLGGDDRIKTSYSVAKEVNGIKKVDEVILTNAYK
                     GEADTISAAPVSVRDIAPIVLTDGKSVPFSTSNVKTYAVGGSISMSTSLVNKTNAKRL
                     GGSDRYDTNKKVIKEFYPDASEFYLSDGYDLVNALTGSTIAKENPIVLVSESSDKSIL
                     AGADKITRLGSISDSVYNKCVSAAQNNGDSSTKGESPMKNETSILGQPTASLEACLKW
                     AKSKKANDLFIELIPILYDTAVQEGVNPVLAVAQSAKETGFCNFGGVLDASFKNPCGL
                     KTSVGGSDTDKNAHSRFDTWEEGILAQIQHLCLYAGQDGYPLSNPVDPRHEKSLFGKA
                     KTVESLSNNWAGGQYGQDLVRMMGEIEATK"
BASE COUNT         8695 a         4286 c         2673 g        10319 t
ORIGIN      
        1 acaaagattc ttaaaaagaa tatactgaaa gcaggtttat ttttcaatta ttacaaaaaa
       61 taaataaagg gtcatctcaa aattgatttt ttaatcaaat tttaagttta accccttatc
      121 ttaattgtgg catacttaaa aatattttaa ctataaatat ctttaagtat agatatgttt
      181 atttatccta aaatcttctt catatcttct tcaggagtag atataggtgt caatgcaaat
      241 ttatcaacca atatatctaa tacattctta gataagaatg ctggcaaaga aggccctata
      301 tagatatttt tgatacctaa atgaagtagt gtaagaagta tacaaacagc tttctgctca
      361 taccatgaca gtaccatgct aagaggcaaa tcatttactc cacattcaaa agcatttgcc
      421 aatgaaactg ccactttaat tgcactataa gcatcattac attgtcccat atccattaaa
      481 cgaggtaagc cacctatatc cccaaggtct aaatcattaa atctatattt tccacatgca
      541 agtgtcaata taacagtgtc tttaggagct tgttttacaa attcagtgta ataatttctt
      601 ccaggttttg caccatcaca acctccaacc aataaaaagt gtcttatagc tccactcttt
      661 acagcatcta ctactttatc ttgtacagat gaaactgtac ctcttgcaaa accagtagtt
      721 aatgtatctc ctccattaat accagtcata tgaacatctt ctctatatcc tccaagttca
      781 agagcttttt ctattacact agaaaaatct ttatcttcat caatatgaac catacctgga
      841 taagcaacta cttctgtagt aaatactcta tctctataac tatctttaac aggcattaaa
      901 cagtttgttg taaataatat tggagcagga atattatcaa attctttttg ctggttttgc
      961 catgctgtac caaaattacc ttttagatgt ttatattctt ttagttttgg atatgcatgt
     1021 gctggtaata actcaccatg agtatatata tttataccct tatctttcgt ttgttctaat
     1081 aatttgtgta aatcataaag gtcatgtcct gtaactacaa taaatggccc tttctcgatt
     1141 ttcatagata cctgagtcgg ttctggtgtt ccataagttt cagtatttgc tctatcaagc
     1201 aattccatac attttaagtt tatttctcca gttttcataa ctagacctaa aagttcgtca
     1261 atagacaagt ctcctccaac tgctattaat ccttcataga aaaattcatt tacgtctttg
     1321 tctgtataac caagtaccat tgcatgatat gcataggcag ccatacctct taatccaaat
     1381 aagattaatg attttaatga acgtatgtcc tcattatcat tccataaatc cttcatatca
     1441 tagttatcat ttctattaca agttgttgaa caatctgaac atctaggtac aagtttactt
     1501 ttttctacct cagttttttc aatttgtctt tttatagctt catcatcaaa acttacattc
     1561 gttatagtag tgaatagccc ctctatcata attttatctg tttcacttgt tggtctattc
     1621 ttatttccaa tagttgctct tgaaagacca atcaacactc ctgtaagttc gtcttgtaag
     1681 ttagctgtac cccccttttt accacagaca ccatttatag tacaaccttt acctcctgct
     1741 gtttgttcac attgaaaaca aaacattttt tccatttttt attcctccct ttttatttat
     1801 taaaatttat tatatttttc tataattcac ttaattaaat ttctccatct acaatttttc
     1861 catctacaga tatagtaact atttgccatg gtatcatttt tccactattt tgtagagctg
     1921 tcttaacagc atttactata ccaccacagc aaggaacttc catacgtgtt actactatat
     1981 ttttaatatc attttctttt aaaatctctg taagtttttc agcatagtca acttcatcaa
     2041 gtttagggca tccgattaat gtaaccttat ttttcataaa tttattatga aaatttccat
     2101 atgcatacgc tgtacagtct gctgctatta aaaaacttgc attctttaaa tatgatgcat
     2161 tagttggtac taattttatt tgtactggcc attgattaag ttgagaacct tttatctcta
     2221 ctatatcatt gcttatagac gtactatttg aaacctctct atttattgac ttagactgag
     2281 aacctggaca tccacatgct aaagttttct tttgtgcttt ctttgcttcc atatttgctt
     2341 ttactgcagc ttcatcatat tctgctgctt ctctatactc aaatgaaatc gcatttgttg
     2401 gacaagttgg cagacaatct cccagcccat cacaatagtc atctcttaaa agttttgctt
     2461 ttccatttac cattcctata gcttcttcat gacaagcttc aacacatagt ccacaaccat
     2521 tacacttttc ctcatcaatt ttaattattt ttctcatcat ttatatcacc tcataaaatt
     2581 tttttaattt aaagtattga tttcttatat atttattttt aatacctata tttctttatt
     2641 tcaaatttct tgtagtagga tttttatatc cccttgcttt tataaactta gtatagtaca
     2701 atgaaaacaa attatcggtt gcatatgcaa caaggaagtg attatttgaa aaaattttta
     2761 gaaaagcata tagacttact taaaaaaatt cctctatttg aaggaattaa aagtgatgaa
     2821 cttgaagaaa tgttggaatg tttaggtgtt gtagataaga cttacccaaa agatagtata
     2881 atattctctg ttggaagtga agttacttct attggaatta tgcttaaagg aagtgctcat
     2941 ataataaagg aagatatcga agggaatagg aatattgtag ctgaactctt gcctggagat
     3001 ttatttggag aggtattcgc atgtactaga ctacataaaa gtaatgttac agtaaccaca
     3061 acatcttctt gcgaagtttt atttataaag tttaaaagtg taactggtat ttgttcatct
     3121 gcatgtgtat ttcataatag gcttgttgaa aatatgctac aattaatagc tgaaaaaaac
     3181 attttacttc acaataaaat agaattactt tctcgtaaaa caactcgcga gaaattgctt
     3241 atgtattttt caaaacagat agaacaaaca ggtagccacc agtttacaat tcccttttca
     3301 agaaatgaaa tggctgattt tttatgtgtt gacagaagtt ctatgtcaag agagcttggc
     3361 aagatgcgag atgaaggctt gttatctttt aacaaaaata aatttgaaat cttttatttt
     3421 caaaacaagt tagatatcta ggcttgtata atatctgtat tcaaataata ttgtttttta
     3481 ttagttattt tctagaaacc tttatacaaa aaaggagtat taataattaa tgctccttta
     3541 gctatactat aaattaaact tttatctatc cattgatatc aatggctatt ttcattccaa
     3601 gtttataggc ttttttcaat accttaaaat tttttttagt ggaatttctt tcaaatgctt
     3661 tggttacaac tatagttttg tagtgattta atcccatgcc ctttaaatgt tttgcagtat
     3721 acttaaaata ttttttaaat aaaaacgaaa taggagctgc ttgagtatag accatgatta
     3781 actttttttg accaaaccta ggtatatgtt tagacttatt tatctctgtt aaaggataca
     3841 atctatctaa aagtagcttt gtttgagctg atacctgaca tatatatact ggcgaaccaa
     3901 ttattatata ttcagcactt tttatatctt catatatgtc aatcatatcg tctttaaatg
     3961 cacaagtagg aacttttcta cagtatagac agccttgaca tcctttaata ttcatatcat
     4021 ttagataata ttttttaact tcaacattat tatcatttac accttttatt atctcatcaa
     4081 gtattgtgtc tacattccct ttttttcttg gactgcccat aaaggctaat acttttacca
     4141 tattaagttc tcctgtctaa tttatttttc taaatttaat ctaaagcaca ctttattcta
     4201 aaaatatatt ttatatactt tgtatttgtt ttatttacat tttatattac aaaagtatat
     4261 caaaaaataa ttcttaacac aattatttaa aagtagtata gcaatttaat acaattattt
     4321 attaaatttt aatgctcctg tagggcatac cattacacaa ggtgaatcca taccacactt
     4381 atatctctca atacaaccat tacacttatt tgccttacca tcatttccat aaattaaggc
     4441 tccatattca caagcttttt cacaaagttt acaccctata cagttttgat tgtctaatac
     4501 aatgaatcct tcttcttttt taaaacaatt tttaggacat acacctaaac attttggctc
     4561 attacaatgt atacatgcta atgaaaaata ttctatattt tcaccttcct ctattgtaaa
     4621 tacatgtcta aaaagttcac cattgtctat attattatca ttttggtcta tacatgcaag
     4681 caaacatgct ccacatgctg tacataattc tttattaaat ttaaataaac ttttcattat
     4741 atctctccat ttatctattc attttttcaa tcttacactt acaacttcta tacgcaggga
     4801 atccactata agggtcaaaa ttatcacaaa taatactatt tatatcagct tctctatatc
     4861 catgatacat atgtatattc ttatataaga ctctatcagt aacattgact tttacatcca
     4921 aacttcctat cttagtagtt atttttatat aatctccact gataacaccc aattcttttg
     4981 catctaagct agaaatatca caattagctt cattttttaa tattcttgaa agtttgatgt
     5041 catgaagtct tgaatgtagt gcatttgaaa ttcttgctcc tgatgtcaat aaatgagtat
     5101 attcatcatc tatattatca ttaaatcctc tatatgttgg tagtgcatcc agcccaaaat
     5161 ctttatattt ttcaataatt gtagatttta attcaaacct agaagtgcta gtatcatacc
     5221 cttctcttgt atatgaacct actatatatg gcttaaaatc tttacatttt aatggtaaat
     5281 cactttcttt aagtttatct acatctacac atgtattttt aaacatatgt cttacacttg
     5341 cttcataacc tgcttttaat aaatcatcat ctatattcat agcatttgct aaatctgaaa
     5401 gtatttctac atctgatttt gaatcgtata atttatcaat aacttttttg gtatatgtaa
     5461 tataccctcc tggataactc ttacattctt ctctctcaaa tgatgtacat gcaggtaaaa
     5521 gtatatctgc atattttgat gtatctgtca taaataaatc tatattcaca aaaaaatcta
     5581 cactattcaa cgctttaaaa agtctccctg aattaggaaa cattctagca tttatcccat
     5641 gacaaaacaa agctcttatt tcatatggtg tcttttcttc tatttgccta ggtaaatcca
     5701 tagcttgaaa ttgagtcacc atttcattcc atagaggaaa tctttctcca ccaattcttt
     5761 catttatatt tttaggtaga acttctttta caaactcttt ttctagtgtt tggaatccac
     5821 ctatcttacc agcaaagtaa tcttgttttg gtatgtttcc acctattcta tcataattac
     5881 ctgttatagc ggataaagac atcattgctc tataattttg aaatccattt tgatgatgaa
     5941 ctattggagc tgcactttca tttatacaaa atggaccttg tgacaacatc tcaacagctt
     6001 ttactatatt atctttagaa actcctgtaa ttctacttac agtatctaaa tcaaataatt
     6061 ttacatattc tttatattca ttaaatccat gtacatgctt ttctatgtat tctctatcta
     6121 ttaaatcctt ttgtataaat agattcgcca ttccaagagc taaagctcca tcagttccag
     6181 gtttaattct taaatgtaaa tctgctagtt tttgagttgt cggtgttatt ctagggtcta
     6241 caattataaa ttttagacct ttttctttta attttattag attcacacta tttaaaaatt
     6301 tagagtagta tggattaaat ccccacccta aataagtttt tgcattctcc atatcttgca
     6361 ttgtaaagtt tcttccagta gcaacatttc cagccatctg tgctgatgtt gaacatgaac
     6421 tagattctgt accataatta ggtgaaccaa atgaagatgc aaacctttgt aaaaaaggtc
     6481 tataccactt tccataaccc gtaaaaaaag ctacatgctt tgctccatac tcagatttta
     6541 ctctatttaa tttttctgct atttctctat aagcttcatc ccatgatatt ggtttaaatt
     6601 ttccttcacc tctttttcca attcttttta gaggtgtttt tattctatct tctctataga
     6661 tatattctct attgcaaaga cctttggtac ataacaaccc tttattcatt ggatgttctt
     6721 ttgtaccttc tatttttatt actttaccat ctttaacata tgcatcaatt ccacaatgta
     6781 ctcctggcga acaaatatca cataaagtcc tctttatttc tatacctgtt tccttacagg
     6841 gtattttagc tttaagttgt ttataactat ccatattaac attttccttt cccaataata
     6901 aatacaaatt attatactta ttatacattc tttttattca aatatcatac tttatatttt
     6961 atacagtgta atttctaaga atatttttaa taataagatt ttatatttat taataatcta
     7021 tttatataat tatttttcat tcatagttta taagataatt caataaaaat ctaatagtaa
     7081 attgttattt acacatatat cgtgatagat aaaagctatt catatctgcc ttaattttag
     7141 tcttaaattt agagtgtaaa ttgaatgcat ttagattata atatatacag tgaggtaagg
     7201 tgaataatat gaaaatagat atgatatata aactaataag aagaaataca gaaccatcaa
     7261 gatggtcaag aggtgtaaaa tattatacta atcaattagt tgattatata gacatagata
     7321 ttgatgacct acaaaataat ctgttgacat ttcgtggtgg agtcaattct gaagataatt
     7381 atagctttta ttttaattca ataactatag atataaagca ctttaagata attgaaaatg
     7441 attgtagttg cgctgactgt caatcaaagg gcaaaagtat ttttatatgt aagcatatag
     7501 ttgctattgt gataaaaagc ttaaaacaaa taaaaaataa aaaagatgtt cctcctattc
     7561 taatagatat attaggtgca gataaggata agctttctaa tacaaattct aaaaccagct
     7621 tagatttaag caagcaatta ctttctttgt tagacaatac taaaagagaa agggttaatt
     7681 tagatgtatt gataaatgtg gattcaaata aagcaattgc tgaatttaaa ataggaatta
     7741 ataaaatgta cattttaaaa aacattactg aatttgcata ttcaaaactt aatggatatg
     7801 aattagattt tgggaagaaa tttacttaca accccaacac tcattatttt gctgatgaag
     7861 atgagaagtt agtagatatt atagaagaat atgggacaaa tctttcatac aacttttctt
     7921 catcacattc aaaaaaattt atggagataa atgcaagctc tttaaaacaa tttttaaatg
     7981 ctattaagta caaggatttt acccttattt tttctaaaga ggaatatcat ccaaagatat
     8041 taaatgatac tctacctgta aggtttgatt ttaaaaatat agaagataaa atagtcttat
     8101 attctaaaga ttcactacct gttccactta ctaaaaaaaa tgatgtactt ttatatgatg
     8161 gcaatatcta tttattatca cataaaaaag ctcatgatta ttcaaaaata tatcaaatat
     8221 tatcaaagac taaagagtta aaatttgata aagaggattc taaggatgtt ttaggattgc
     8281 tagttcctag attaaaatct atatctcaag aagttcattt agatgataac ataaaaaata
     8341 atataacaaa agattttaaa tctgagttct actttgatat gattgatggc aacatatgtt
     8401 gtgatgtaaa gtatatatat gatgatgaag ataaaaaatt tgtgctcccc aacatacaaa
     8461 aggaagctac tattgaaaat aaacttactt cttcacaatt tacaaaggaa aataatcatt
     8521 atgtgtttaa aggaactgac catgacctat ttacattttt agatttacaa ttagaatctc
     8581 taaaggaatt tggagatata tattattctg agaagtttaa attgaaaaaa atatataacg
     8641 cttcatcaat tcaagcttca atcaatcaga ctaaccagaa ttacctagaa tttaatttta
     8701 atatatctga tataaatcca aaagagtaca aggatatttt gaaagctttt caagaaaaac
     8761 gtacttttta taaacttaaa gatggaaact ttatagattt atcagaaaga gaaacaaaag
     8821 atttttttga attagttgaa aatctagata taatggataa atcaaaaaat aataaaattc
     8881 ataaaaataa agctctatat ataaatgatg taatacgtag taaaaattta aagtttatag
     8941 atggtaaaaa agaactagac agtatatgtg aaaaatttag aaattttgat agcataaatt
     9001 tagaaattcc cgcaaattta aatgcaaacc ttcgtgacta tcaaataaat ggcctcaatt
     9061 ggtttaaagt tttagattat tataaatttg gtggtatatt agctgatgaa atgggtcttg
     9121 gtaaaacaat acagactatt gcatttttat tatcattatc taataaaaaa agtcttattg
     9181 ttacaccgac atcactcatt tataattgga aaaatgaatt tgaaaagttt gctccagata
     9241 taaaagtttt attgatacat ggaaataaaa gagatagaga aaaatgtttt atggaattag
     9301 aaaattttga tgtaatactt acaacatatg gtactcttag aaatgattta gacaaatata
     9361 gtgaaataaa gtttgattat tgtatattag acgaagctca aaatataaaa aatccagtgg
     9421 ctttagtaac tgagtctgta aaaagtataa atgcagaaaa taaatttgca ctcactggta
     9481 cacctatgga aaataatcta ctagaattat ggtctatatt tgattttata atgcctggct
     9541 atctatatag taaagcaaag tttcaggaat tgtttataaa taaagaagat aatgttaaaa
     9601 acctgaaaaa attgataaaa ccttttattc ttagaagaag taaaaaacaa gttatgaaag
     9661 aacttccaga taagattgaa aaaaactttt ttgttgaatt gaataaagag cagaaaaaga
     9721 tatatagtgt atattcaaaa gatatacaag ataaaatgaa agataaaaat ctaaagaaag
     9781 ataaaatagt tatattctct tatttaacta aactaagaca actttgttta gacccaagta
     9841 tagtagtaaa agattacaat aaaaaaagtt caaaaattga aacatgttta gaaatactta
     9901 gggatagtat aaacgaaaac cacaaaatat tattgttttc acaatttact tctgtgctta
     9961 agaacataag taaggaactt gataagtata aaattaaata ccattatata gatggtaaaa
    10021 ctaatgccaa agaaagactt gaacttgtag atgagttcaa taatagtatg gataaaaaag
    10081 tttttttaat atctttaaaa gcaggcggaa ctggtttaaa tcttacatct gctgacatgg
    10141 taattcactt tgacccatgg tggaatccat cagtagaaaa tcaagctagt gatagagctc
    10201 atagatttgg tcaaaaaaat tcagtacaag ttataaaatt gattgctaaa gggactatag
    10261 aagaaaaaat aataaaatta caagaaagta aaaaagagct tataaatcaa tttataaatg
    10321 gagaactttc taatgaaggt gtattaaaaa gtctatctga tgaggagata ataaacttat
    10381 ttaactaaat tatttaatcc tacagcttaa ctatatgaat ttgttctaac atttatatag
    10441 ttaagctaaa atatctgaat aaataaaatt taatattgtt atttaatccc aactttacaa
    10501 tctaatgata tctctttcta tacatattgc atatacaaat gtaataactt gactattaga
    10561 atattttgtt ttaacaatcc tatttcatca taataatgaa gtatatgtac actcactttt
    10621 gtaagttttg caatccattt tacagttttc attattgtaa attttggtta aattattcct
    10681 ctaaaaattt ctttgctaca tttgaaaaat ctaatccatt cgataaaatt gcaggatgac
    10741 cacctttatc aaacaaatac attttaccat ttttaatctt ttcaagaagt aatgaaaaag
    10801 tattttcaaa aaaatcacta gatattagag atacaaactc atcttctcta cttcctgtta
    10861 aaagcacctc tggttgcata gtttctagag gcttatgaaa aaactttcct atagttttat
    10921 aatgttcaaa aatagcttct gtatcattat ctacaacttt ttcccagttt tctccttgat
    10981 tataaatata aaaagctttt gcaccatcat cttgcttaga tgcttcacgc tctattttca
    11041 cattttgtac aaatgactct agaggaactt ctccttcaaa actatctgca attaccttat
    11101 tcactaaatc aggtctctca agtgcaacat ttaatgcaac aagagctcct ccactactac
    11161 caattatgtt tactttttta tagttcattg cttctaaaaa acatatcact tgcatagcct
    11221 catcaaacca taagtcagca gaaaatttat cgaccatttg agattttcca tgacctacaa
    11281 aatcaattaa tattacttta tattcatctt tataaaaatt gattatatca ttaaacatct
    11341 tggaagaggc tgtatttcca tgtaaaaaaa gtaatggtgt accatcccca atttcattat
    11401 agtagcactg tttgtcgtta tagttaaaat atgccatata tttcccccct ttaatatccc
    11461 tataggtata tatttattac ttatatttaa aatgactatt cccttagcca cttaaaaata
    11521 gtataactta gaatgtagat aaaatcaaca ttccttgcta atttatctat tgtttgtcta
    11581 atttattata atttaacttc tgtaaaattt cttttttgta cgaaaatatt ccttccattt
    11641 acaacaatca ttcaaatgaa gtataacatg acgagtagga ggcataagca aaattccagc
    11701 aatatcttta ttcccccaaa agtccaacaa ccaaatagaa tcttcatgct ttattactcc
    11761 tccagtcacc aaaatccttt ctaagtcaat tggagagacc tttcgtttca tatcttcagc
    11821 tttcaaatcc ttaacaactt ctattgtgtt ttttgcaact tcatttcgat aacaaagcaa
    11881 ttcatcaata ttgactaaat tatcgtatat ctcttttcac tttaaccatt tttttcttta
    11941 taggtatctc cttatatttt tttaatatca aatctccctt ataatttaat atatctacat
    12001 atgaacataa atcttgaact tcaattacac ctatatcatc attagttata ccttttatat
    12061 tactaaatgc accaactata tctaaaactc ttattttctt cttcttacca gcatttaaat
    12121 ataatttaac aacttcactt tctatattta gttttatatc tttacttttg taagaatttt
    12181 tatcaacttt ttttctcttt gatacttttt ttaggattcc ttttgaatat tcttcaaaca
    12241 aaactttgcc atgtgtaatt ctactttttt ctatatcttt taattcattt attttatatc
    12301 ctatatacgt ttctatattc tctatatatt tttcatcctt ttcagttact atagtaattg
    12361 cctttccact atttcctttt ctcccagttc tacctattct atgtatatag ttttctttat
    12421 cttgtggcac atcataattt attactaaag aaatatcatc aatatgtatt cctctggacg
    12481 ctacatcact actaactaat atattaaatt tttggttttt aaaatcctta attacaaaaa
    12541 ttcttctttc ttgtgataaa tctgcatgta actctcttat taaaaaaccc tctttactca
    12601 tcttgttata taatttagat actttctctt tggtattaca aaatattatc acactctttg
    12661 gattttcgct gtatattaat tcttttaaaa ggatgtactt ctctcttcca tttgccttga
    12721 ttattttgtc atctatttgt cttgtttttt gattagtatc gctctcattt tcttctatat
    12781 ttataacact gtaatctagc atatattttt cacatatata ttttatctct tcatctattg
    12841 tagctgaaaa aagacttact attttctcct taggtaaatt taataatatt ttttccattt
    12901 gttcaacaaa acctttatta aacattttat ctgcctcatc tattactaaa tactttacat
    12961 tttccaactt aatagaacct ctatttatat ggtcaagtat tctgcctgga gtagctacaa
    13021 caatatgaac cctttgtttc aattcagcta tttggtcttt tatagattgt ttgccaaata
    13081 tagcactaca tctcaccttc tttagtctcc ctatatctga tatctcatct tttacttgca
    13141 aggctaattc tcttgttgga accactataa gagcttgaat attattgtaa tctacattta
    13201 tattctcgca taaaggtatt gcaaaacttg ctgtctttcc acttcctgtt tttgacctaa
    13261 ctactaagtt ttgacctttt aataattttg gaataacttc tctttgtact ctagaaggta
    13321 tattatatcc taaactcttc aatgacttaa gtatcttttc atttaactta aatttttcaa
    13381 atgtatattt catatattac caccttatta tatttatctt atctatatat ttttctacat
    13441 tatattatta taatacaagg ttctactatt tttatattat ttattagact gaagataaaa
    13501 gtatatactc tataaaccta tagtttagta gcaaattaat taaattttta tatttaaata
    13561 cctccttaaa tatactgata aatcaatatt ttcaaggagg tatctcttgt caataatcaa
    13621 actaatctat cttttgaaag tttaaattat actaatacat tgtattttta tttatatcta
    13681 atattaaaag gtattaatca atacctaaat caccataatc tcacatattt atatctttat
    13741 tcaatatacc ttctgtaatc aatcgtgcta tataatcata acctgattca tttgctttgt
    13801 tacaatcatt cacattatca cagaaaaaac tttcaatctg tattgcaact ggatttgtat
    13861 ttctcaatat atataaattt tctctttttt tagctcctct attttcaaat aattctccaa
    13921 gtctattaga tactctttta gcatattcta atccttcagt gccatagtaa agcacttctg
    13981 taccacattg tgttccatca gctttattta aatgtagctc tactaataaa tcgtatttac
    14041 cactgtttac ttttggaact ctatagaaaa attcttctct ttctgacata aagtgccttt
    14101 ctggacaaat tattacatct actgtattac cttgacttat aagcatctca gctaacatag
    14161 gagctaattt tttattataa ttgtattcat caactatacc atttacacct gtacttttac
    14221 caccttttag tatactatga cctactgtta tgcatatttt catttacttt tttcaccttc
    14281 ttttaagcca atttttatat aatagtaata taattttaaa gttattacta ataataatat
    14341 attaattgaa gaaggtaaaa gttctccgtg attatcattt ttttaacata tttgtcttat
    14401 tttatcataa atctatattt tttaaatatt atactatact tattatttat aattgtttaa
    14461 ttaatcattt atatatatga taaatatatc aatatctatt ttcctttaac caaatgctat
    14521 tttaataagt ttaagtttta gctttaaaat acttaataat acaataaaag tgatgtattt
    14581 acaaataatt cttctggatt taattcattt attgatgtat gttcaatttt tatatttata
    14641 tttttcatat cttcataaat aattccaact ggagaacaaa tgaaacaaga aaatccaaaa
    14701 gtttttgtta ttttttttac cttattatca ccaaaactta tttgcttttt acaatcgttt
    14761 atattttcat tgttattttg tatatcattt ttataatact tatctttttt aatgtcatta
    14821 gacttattta ctgttttaaa attactccta tttttacaac tatagtctat ttcaacttct
    14881 atatacccaa aaactatcaa gttacataat ttcaaattac tacctttgtt tataagattt
    14941 ttcataggtt taacatcaaa tattttacta tttacattta cttttaatat atctcttaga
    15001 tttttaggag cttttaaagt attacttata ttcaattgtt tgaatacaat tttatttaca
    15061 ttagtataaa ctctattact ttctattgat attcttgttg ggaatttatc tagattacac
    15121 ttataataat agtatatatt agcactattc tttataatcc tatgtgcaga atttggtaaa
    15181 actacactct taaaagatat tattttttta caacttgcat ctattctccc tatgtaaaaa
    15241 ccttgctgtg ggtctaatcc acgttttata atgccattaa cttttaccgt attatttata
    15301 aactctatat atttagaaat ataatctttg aaaaatatat ttccagtttt gtacctactt
    15361 gtatttgtaa caataatagt gtaggtcaaa atctcaaata cagatgcaca ttttttatca
    15421 acatattttt ctacttttat aatacttcca tttgcatcat ttgaaaatac atcattaaaa
    15481 gtagttttcg ttattctcat tgcatccacc tttctataat ttaaagatta ctctatcttg
    15541 gaactatttt cataacttat aattgcagta acactaataa ataaattatc atctaaaata
    15601 gatgaagtaa tatcttgtat aagatacttt aaattaattt tatctttatt ttctatcttt
    15661 ctaggcattt gtataaatgt actaaagggt atttttaatt taaatgtatt ttttttacta
    15721 gaatttctat ttcttttatt gcctgcataa tctaatattg catcaatatc tatatcacca
    15781 actaaaatca acttctttcc tgtaagttgt tcatcttcta atgatataca ttctatagta
    15841 tcaacaagac ttatattgtt aatattcata ttgtaaacat atgtctctaa tgaatttatt
    15901 acagtttttt tagaagctaa tttaaaattt ttatatgtat taaattgaga taaataaaga
    15961 gtcttttcta gtttttcaca tctttcaagg ttgtatatta ccttattatt ttcatacata
    16021 atcgcatcaa tctccctgta tctatatatt aatactattc ataaaaatta tttcaaatta
    16081 ccatttttta ttattcacta tctagttgtt atttactttt aagaaagaat ccttttttac
    16141 ttcattgatt tcaatttaca agtaaaagtc tttctgaaaa aaaaatatat atatctctat
    16201 tcactttttt ataagtaatt ataattttta tagactaacg gtcagcctta agggaaaagg
    16261 tgttctttaa gtgcagaatt tagataaagt aaataatcct tttataaata attccaagtt
    16321 ttaacttaaa taatttgatt tacagtataa agtaaatttt gaaagataat gatatcgatt
    16381 taataattac aagtaaaagc atgtcaaaat aaggctatga ttttaaaaat catagcctta
    16441 tcaataaaaa tttaatcaaa gttacatttc actttgaatt tcactgtcta ttaattgaag
    16501 attatactgt tgaattaatt ctattagacg agctgcatat atactattgc cattttcatc
    16561 cttcgcagta ctataacctg ccttttctaa tgctttagct tgatatatat acgtttttgc
    16621 atcaaataca ccattgtttt tatacctttt atttgtggct aaaaattttg catggtcttt
    16681 tatagattca tttttatcat catatattct aaatttatca tctataattg tatctaaatg
    16741 ttctttcgtt tctaaagtca ctgacttgcc tttccaataa gcgtcagcct ttataccaaa
    16801 tagattgtta tattgttttg ccaaagttga cttgccccag ctagattcta ttatagcttg
    16861 tgcaatagtt atagatggaa gtattttata atccttataa ttttgtattg cttctgtttt
    16921 aatttcatca ataaacttca tatattttgt atcagacttc aatctttctg gttttatacc
    16981 ataatcctta agattgtcta tataattatc aaccaatctt ttttgtctat tactaaattc
    17041 taactcatcc aaaatatcac ttaacttatt tattttatta tttttagaaa aattcttaac
    17101 aaataaatct gatacttctt gtatctgtga atccttaacg ttttttaagt tatttttatt
    17161 taacacagca acaatggaag ctacatactt ccagttaact tgtgcattat ttttacttgc
    17221 taaatcagca tattttatat acttactgac atttatatca tcctttttta tctctctaga
    17281 tgaatattcc ttcactaata cacctattaa aattgatatt atcagtatta taacaatgaa
    17341 ttttttattc ttcataaata ttgtcctccc ttatactatc tgctgaatat aatatacctt
    17401 acttttgtga catattcttt acgaaatttt gaaaatttta tgaagattta tgaaatatat
    17461 ttaattaaaa gcactactat tgttataata tatagttttc tatcacacaa aatagttaaa
    17521 ataaataaat aattagatag cttattttaa ctatttatat aataaaaata ttattaaatc
    17581 tatttatcaa tttttataca tttttctaat atatattttt tcccatatac aataattttt
    17641 attggtttac cttttaaatt ttctatttta attaaatcac tacatacatc tacattaata
    17701 atattatctc tataatttat attgaaagaa taatttttcc actcttttgg caacttaggg
    17761 tcaaaagata gagtttcatt ccatgttctc atgcctgcaa atccatgtac aatggaaagc
    17821 catgaaccag acattgaagt tatgtgcagt ccatcatttg tatcattatt gtaattatct
    17881 aaatcaagcc tagctgttct tgaatataat tcataagcct tttctaggtt atctatttct
    17941 gaggcaataa tagaataaat actagaagat aaagaagatt catgtacagt atatttttca
    18001 tagaagtcaa aatttctctt tttttcttct ttagtaaatc tatttccaaa atagtaaatt
    18061 ccctgtaata catctgcttg ttttataaaa cacgacctaa gaatcttatc ccaagaccaa
    18121 ttttgattaa gaggaaaatt ttcttttggt aaatccttta cttctacaaa ttctttatct
    18181 aaaaaattat cattttgaat tattatttgt agttcctcat catacagcag atacatttta
    18241 tttgatatta ttttccaaaa ctcaacttct tccctagtca cattatttct gtcaatacat
    18301 tcattacact ctttctctaa tcgtaagata ttttctacag cataatctaa acaccattta
    18361 gctatataat tagtatacca attattattg atattgtttt catactcgtt tggacctgtt
    18421 actccatgta tcatatacaa atcttttttt gtagacaaat gaactctact tgcccaaaat
    18481 ctagctattt ctattattac atctattcct ttttctttta tatatttata gtcacctgta
    18541 taatttgtat agttatatat tgcatatact atacttccat ttcggtggat ttcctcaaat
    18601 gttatctccc attcattatg acattctact ccatcaaatg ttaccattgg atataaagca
    18661 cctttaagac ctagtttttt tgcattatcc tttgcctgtt ctaactgatt gtacctatat
    18721 tctaaaagat ttctagttac ctccttatct gctacaccta gatatactgg aacacaaaac
    18781 gcttctgtat cccaataagt agctcctcca tacttttcac cagtaaatcc ttttggacct
    18841 atatttaatc gcgaatcttc tccataatat gttgaaaata gttggaataa attatatctt
    18901 ataccttgtt ggctactttt atctccattt atttttacat ctgcactttc ccatctttta
    18961 ttccataact ttatatgttc atttaatata tcatcataac ttttagatag ttctctattt
    19021 aaaatgtctt cacattttct taatagctca ctttccttat aatctctagt tgaggttata
    19081 gatacatatt tttctatact tgcaacctca ttttttaaaa catccattgt tatcgaattt
    19141 tcacagtaat tataatcact ctcatgagat acagcttcta catcaccatt tgtcttaata
    19201 tacatcattg aacccacagt gaatactggt gtattaaatg cattctcctt tgttcgagaa
    19261 agaatactcc ctttatattc taaagaactt gaagaaattc cattccaaaa cttttcctca
    19321 tagtttgaat cttcattttt tatatttgaa tctaaatatg gtacaaattt tattttagta
    19381 ttagaattta atgattttat actgtattta attactgcaa gttccttaac agatatactt
    19441 aaaaacctag taaccctaac ttcaaatttt ttatcattta tatttacaat aaacctacga
    19501 tttaaaatac catttttcat atctaattct ctatgaaaat cttctacttt acattttgct
    19561 aaatctaatt ctacatcatc tatgaaaatt cttacaccta tatagttgac tgaattagga
    19621 attttaccaa agtattctgg atacccattt ttccaccaac caacacgagt tttatctgga
    19681 aaccatactc cagctatata agaccccttg tgtgtatctc cactataatt ttcctcatag
    19741 tttcctctca tacccatata tccattgcct atactcatta tggactctgc taatctattt
    19801 tctttgattt caatcttatc ttgtataatc ttccactcat ctacattact aaataaacat
    19861 ttcatataat tctcctaact atatactcat tatacttttc ttgtatatat tcaaacttta
    19921 attgatttgt tgaatctaca actaaatttg ctttttttaa gttttcataa tttccaacac
    19981 caacagaaaa catattagct gagtttatag cgtctatacc tgcacttgca tcctctatcc
    20041 ctatgcaatt ttgtggattt acattaagcc ctttcgctga cataagaaat atctctggat
    20101 gtggtttatt gtttttacat tttcctgcat cagctataaa atcaaatttg tcacttatac
    20161 ccaaatgatt taatacattt attgcattct tacttgcaga agatagacct atctttatat
    20221 tattagattt tacatcaatt aacaagcttt ctatgcctgg caaaatatca tttgaagtta
    20281 tctcatctat caatgataca tagtaattat ttttttcttc agccattctc actttttctt
    20341 cttctgaaaa tgaatatttc ttatttccaa attctaatat cctgtccaat gactccattc
    20401 tacttatgcc ttttaaagat tcattaaatt ttgtgtctat atctatccca actttatgag
    20461 ctagttttct ccaagccata tagtgataat atgcagtatc tgtaattaca ccatctaaat
    20521 caaatataaa tgcttcaatc attcttattt cctctttcta caagaaattt ttagactaat
    20581 aatcaattta ttttctatct tgtatttaag atattattta taaaattatc tactctaact
    20641 cagtctttaa tttaaaatct actttcaatt cactcacttg tagaccatat tttttatcgt
    20701 ttataaagac ttgtttaaac tgtataataa tttaacatga aatctaaaat aaatactttt
    20761 ttgtattatt ttaaaataat gaacttgtat ttctctaaca ttagttcttc ttttaaatca
    20821 actatttctt cttcatataa atcttttact tttttatttg ctaaataatc tggtaacgtc
    20881 aaaattatat ttttagatga attatttatt attatagaaa tagattcttt tttcaatata
    20941 atcgttccat tttccttatc tgctaacacc cattcatttg atatagacct aagttcttta
    21001 ttttctttac gaagtctaat aatcttcttc ataaaatcaa atatatcctt attccatttt
    21061 tcttcattcc attccataca ttttctttga ccttcagata ttgaacttaa ttctccttcc
    21121 attccgattt catcaccata gtatatacaa ggacatcctg tttgtacaaa cataaacata
    21181 tatgcaagtt taaatctatc aatatttcca ttagcataag tcaatattct tgtagtatcg
    21241 tgacttccaa gtaaattaaa aataacttca ctaacttgta ttggataact taccattaca
    21301 tcatttatca tatacttaaa ttcttcggcg tttatctcat ctgtacaaaa gaattttttc
    21361 atggcttctg acatcaagta attcataact gaatcaaatt ggtcacccat taaccatggt
    21421 aaactaccat gccaaatttc tcccaatata taaatgtctg gttttacttt tttgacttct
    21481 tctctaaact ttctccaaaa aacatggtct acttcatttg aaacatctaa tctccacgca
    21541 tctatatcaa attcctgtat ccagtacttg cctacatcca ataaatattt tattacttct
    21601 gaattttctg tattaagttt aggcatatat ttttcacacc caaaggtttc ataaggtata
    21661 tttttttcat ctatttgttc tattggagta tcaattttac tcatatcttt tatataaaac
    21721 cagtttttat atcgagaatt ttccttattt tgaactacat cttgccactg tttagaataa
    21781 tatcctatat gattaaaaac agcatccaac attattttta tatttctctt atgcgcttct
    21841 tctattagtt tttttaaagt ctctttatct cctaatgtag ggtctacctc catataatct
    21901 accgtatcat atctatgatt ggtttttcca attgtaatag ggcaaaagta aattccgttt
    21961 attcccaaat cactcaaata atctaagtgt tctatcacac cttgtaaatc tccacctgta
    22021 aaattatctc tggttggaat agctccccat ttatctactc cttctgggtt tatagacggg
    22081 tcaccatttg caaatctatc tggaaatatc tgataccaaa cagtatcttt cacccaattt
    22141 ggcactttag gtatatctat ataatttaaa tatggatatc caaaaaaatt acctatttca
    22201 cataattcct tttcatcatt ttcatttccc aactctatta ttcttttttc tgtaaataaa
    22261 agtttttcct cttgaccttc tattataaag ccatatcgtg agcgtttatt taaaggctta
    22321 cacttagcta tccaatagtc aaataattca gtttctactt ctttactcat tactatattt
    22381 gttccaccag accaaccaac tccagatgca tttaaatttc ctcctcctgc acctccttta
    22441 tcccacacat attggtctcc tattcgtaaa attactcttt tcgcttcatt ttttttagtt
    22501 ctaagtctta tatgtagtgt ttctctatca taaccatagg cataattgct cattggtata
    22561 tgtaatatag cttcttttgt taaactattt tgaattgttc ctttgatttg attacttaaa
    22621 gttatatttt ctttattttt attagacatt ttaaaatttc cctcctttat aataatttat
    22681 ataatctaaa gataggttaa tatattttac tattatatca caataaaaat atataatagt
    22741 aaaatatatc aacaataaac tattcaattt aaaataagct atactattta aatttaattc
    22801 tatttagtta ttttagcaaa ataaaaacct ctagtatttt tctagaggat acaatactat
    22861 aactattaac tttttaacta tctattaatt tacttcaaaa cacactctaa gctatattgt
    22921 tagatttaac tttttctata tttacaaaat aggtttctct ttctttaaat aaagatacat
    22981 atttaaagtc aagatactta agcatatcat aggccacatc aaaatctttg catatatctt
    23041 ctaccctatg agaatcagaa cctacagtaa ctataaatcc tccaagttct ttatatcttt
    23101 ttagaatatc ttcatttgga tgtatagcat ttagtccata tctataacca gaagtattta
    23161 gctctatacc actcccatta ttgattattg actttaagac ctcatcaata acatctttaa
    23221 agtcattgta tataactcta ttatctgaat atattccata cctagatata taatccaaat
    23281 gaccatatat attgtaatct ccaaattcac gattagtttc aagtatgcta ttaaaatatt
    23341 tataatacgc atcttctttg ctaagacctt taaaatacgc ctttgatgga acaggtacat
    23401 tgtcaattgt atgagttgag cacaatacaa aatcaaaagg attagaattt attatttctt
    23461 ctatttcatt ttttagatgt tttctgtaac ccacttcaac acctagtaat aactctatgt
    23521 cctttttata tttttcttta aacttattaa atactggaac atagtcatta taatttatgc
    23581 aaaatcctct atcttcatca tgttctaaat ggtctgtaaa agtcaaatat ctaacaccaa
    23641 tacttatagc tttttttatt gtatcttcca tggaatcctt tccatcactt gaaaaattag
    23701 tatgaatatg attatctatt aaattcatat tatccctccc tttattttat ctatcttaat
    23761 tatacaacaa gtttattgta tacatttgtt aaaagaaaat tataatggtt aaattttatt
    23821 tacatacttt tatttttaca aaaaagagaa gcacctattt ctgattttag aaaatcatta
    23881 aggatacttc tcctaatgtt acttttatat acttcaattt aatttttaaa agtcttaatt
    23941 ttgtaataga gtttagctgt tatatgtcca atcatataaa aagctgtata aagtaaaaca
    24001 tatatatatg cagaatcatt taaaaatata tacattaata ctatataagt tactaaagaa
    24061 acaataagag ataaaatcca attagtctta tttaatacac aaattatccc ttgtatcaaa
    24121 aaagaaattg gaaaaatcaa aaccattccc acaacaaaaa tacttttaga ttgaatatct
    24181 ggaatattaa atatatctgt agcaaaagtt aaaaacatta gcaccattat gactgtagga
    24241 aatatgatac taaaaatatt actctttttc ataataatca atccttattt attggatatt
    24301 tgataatctt caattatttt gttgcctcaa tttctcccat cattcttact aaatcttgtc
    24361 catattgacc tccagcccaa ttattagata aactttcaac tgttttagcc ttaccaaata
    24421 aagatttttc atgtctaggg tctactggat ttgaaagtgg gtaaccatct tgtcctgcat
    24481 ataagcataa atgttgaatt tgagctaata ttccttcttc ccaagtatca aatcttgaat
    24541 gggcattctt atcagtatca gaaccaccta cagaagtttt aagtccacaa ggattcttaa
    24601 atgatgcatc taatactcca ccaaaattac aaaaaccagt ttcttttgca gattgagcta
    24661 ctgccaaaac aggattaaca ccttcctgaa cagcagtatc atataatatt ggtattaact
    24721 ctataaataa atcattagct tttttagatt ttgcccattt taagcacgct tctaaactag
    24781 ctgttggctg acctaatatg cttgtctcat ttttcatagg tgactcacct tttgtagacg
    24841 aatcgccatt attttgtgca gcagaaacac acttattata cacactgtca cttattgaac
    24901 ctaatctagt aatcttatct gctcctgcta atatagactt atcactactt tctgacacca
    24961 atacaattgg attttcctta gcaattgtag aacctgtaag tgcatttact aaatcatatc
    25021 catcactcaa ataaaattct gatgcatctg gataaaactc ttttataact ttcttatttg
    25081 tatcatatct atctgaacca ccaagtcttt tagcattagt tttattaact aaactagtgc
    25141 tcattgatat actcccacct actgcatatg tttttacatt gcttgttgaa aaaggcacac
    25201 tctttccatc tgtaagtact ataggagcta tatctcttac tgatacgggt gcagcactta
    25261 ttgtatctgc ttctccttta tatgcattag ttaatatcac ttcatcaact tttttgatac
    25321 catttacctc tttagcaaca ctataactag tctttattct gtcatctcca cccaatctag
    25381 ttacctgtgc ccctgtgttt tttagttcag tttctactga cttacttatt gataattctt
    25441 tacctattat gtatatcttt ttaacatttt ttaatctttg ttttgtttca tttggtatct
    25501 tatttttttg tgcaagcaaa attggagcat caacagcacc tgcaagacca cttgctgaaa
    25561 gaccatctgc tatagaatta tccatattta ccataattgc tgtatcataa ctctttttat
    25621 ctgctattaa tccagccgtc tcatatatat tgttaccttt aattgtatct gatgttaatg
    25681 catctacatt aggtacattt attagaaata tagctaatgt taaacctaat gctaaaactc
    25741 ttctaattgt tctcatattc ttcccccttg atatatcaag tttaattcaa aaatgactat
    25801 aacatagtat tacatctacg ctatagtcat ttattataat ttaataatta tatatttgtt
    25861 attatatcat aaattacaaa aagtttccat aatattgtat agattaaagg aaaaatttac
    25921 attttagtta caatttacta gatttttgtt ctaatgtacc tatatttagt aac
//
LOCUS       DAYAPA010000049        25444 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.049, whole genome shotgun sequence.
ACCESSION   DAYAPA010000049 DAYAPA010000000
VERSION     DAYAPA010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25444
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.049"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            250..1401
                     /locus_tag="ACGI59_001881"
     CDS             250..1401
                     /locus_tag="ACGI59_001881"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004452985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFG9739667.1"
                     /translation="MELRPRSAKLEEFDKVIELINYVFRISRNHKPTMMEEFPLLLSK
                     NNIENMIIISEDDKVISDVNYLIQDVSIQGNRLKVAAIGGVCTHPDYEKRGYSSKILD
                     KVEEKMFYDGVDIVIISGTRSLYSRRNCSLVKSFYKYTIKPEDVKIAYEIVEFDETNF
                     EKDNDLDKMIELYNQNSTRFIRTRDEFQKLLHAATIAWGPIGYKKVFIKENNNIIGYL
                     IIRTIKKEDSTVGEVAEIGLNSVNVENILKYVANKFGLEYLNYKVHVKNLKDQLKCNG
                     TKSLDYQQGTMKIINFTKLCDSLRSYFSQYVDFELLKYMEFKQVENKYIIKYKEEELV
                     IENLDKLNKLFFEKNEEQYNEFKHLKNIYEFATKAFPVDFPWTANLNYQ"
     gene            1519..2157
                     /locus_tag="ACGI59_001882"
     CDS             1519..2157
                     /locus_tag="ACGI59_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423639.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HFG9739668.1"
                     /translation="MDILDDIAECILNMGVDNIENLVKIAIDKNIDVEDIYEYGLNKG
                     MIGALDKFENKEYYLSEVIVCTDALNKGINLLKGTGKVKKKSKGVILMSVVEGDTHEI
                     GKNIVKVMVEATGYKVIDLGVNRKSEDIIEEAIKNNVDIIGLSSMMTTTMENMKSVID
                     ELNMIEIDKRPKVIIGGGPVSMEFAEEIGADGYSSNAPKAVKLINKLIGGEV"
     gene            2158..3180
                     /locus_tag="ACGI59_001883"
     CDS             2158..3180
                     /locus_tag="ACGI59_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HFG9739669.1"
                     /translation="MFSDMTLLQYINSEDRGFFLPDMGTNGLFLINKKAYEVYDSVDL
                     QLELAKTMDKYFESDFIYSFCDGITFCETLGLETLRPDYDFPSVLTHTITDRDKLRKL
                     DVPDPYTSGRMPLNIESLSVISKSIKKPLYESIQGPFTLAGQLAGATQLLRCIITDKK
                     FVEELLEFTTELVRRYAVAANKAGAKYISIAEPTSVTLSKDRFDEFIVKNLNKIYNEL
                     DCWKGMHICGDTRELLDNMLSCNIDAVSLDQILDYEEIAPMIPRDIVLIGNLDPIKLL
                     GRSKPDKIRRETLKLLKKMRRYDNFLCDFGCNCLNTTPVENLQAAIKAGRISYKELDR
                     IDIDEL"
     gene            3170..3790
                     /locus_tag="ACGI59_001884"
     CDS             3170..3790
                     /locus_tag="ACGI59_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9739670.1"
                     /translation="MNYNSNKNKYLLKDINLDDIFIDKNIVLKFLGYGNRKAPDTISK
                     IVEREIRNINDILDIKIYISEVDINKTPKECKKAFAVLYTIGDKIDIKMNDYMENCNM
                     MAGLALDKIGIVCLDYINEKIKMYLKGKYDSLKISHEIYPGDKDFEVERQKDIYNYVK
                     NKYNSIEIEINDYHQLSPIKSVAMLILMGDEENLESRCSKCPRKCF"
     gene            complement(3914..4600)
                     /locus_tag="ACGI59_001885"
     CDS             complement(3914..4600)
                     /locus_tag="ACGI59_001885"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HFG9739671.1"
                     /translation="MKTYKFIFFDADDTLFDFKKSESHAFKKLLSEFDLEFNFENYIE
                     SYRNISDKLWLDLEKNIITLNELKLLRFELFANKISLDVDSETLSKMYLNFLGECTFL
                     IPGAIDILQYLKKKYTIVIITNGISAVQNKRLENSKIKGYIDGMVVSEELKISKPNPE
                     IFKYALKKFNCHDKSSALIIGDSLTSDVLGGINSGIDTCWLNSNNSINYTNHIPTYEI
                     NTLIELKKLL"
     gene            complement(4614..5159)
                     /locus_tag="ACGI59_001886"
     CDS             complement(4614..5159)
                     /locus_tag="ACGI59_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFG9739672.1"
                     /translation="MNDLNLGEKIAEVRKKQNLSIRDLAKLADVTPSLLSQIERGLAN
                     PSVNSLKSIASSLNVPLFTFFVSEVDKKNLIVRHDNRKKVILPGSKEVIYEILIPDSS
                     GNLEFAIMDLAPNTSSCVDRITHNGDEIAYVLEGEVKLFMDDDEFTLSKGDSVKVPLG
                     TKHKWQNDSNSESKVIFAVIL"
     gene            5387..6634
                     /locus_tag="ACGI59_001887"
     CDS             5387..6634
                     /locus_tag="ACGI59_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HFG9739673.1"
                     /translation="MQITTFGAIFGLLIAIILIIKKFQAVYSLMLGAFIGGLVGGANI
                     TQTVDFMANGAMNISPSILRALASGVLAGSLIKTGAVDKISEQIVKIFGEKRALFSIA
                     ISTMVLAGVGVNLDVSIITVAPIGLYIGRKLNYSKLSILLAMLGGGKAGNIISPNPNT
                     IAVADNFSVNLSSVMMANIIPAIIGVVITVILASILINKGNKVQSYEILEQREDLPSL
                     FKSLCGPIIAIFLLFLGNVSPIVIDPMIALPIGGIVTLIVTGNLNNSREYLTFGLSKM
                     QGVCILLLGTGTIAGIIQMSELQQSTIGALQFLNMPQFLLAPVSGILMSLATASSTAG
                     ATIASSTFHDAIINGGLSPISGASIVNAGSSVFEQLPHGSLFHTSAGSINMDIGERFK
                     LIPYEALIGIVMTIISTSIQLVL"
     gene            6721..8715
                     /locus_tag="ACGI59_001888"
     CDS             6721..8715
                     /locus_tag="ACGI59_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFG9739674.1"
                     /translation="MNKKKIVIIGIIYSFLVVFSLTNMYVNMEYNLNVFEYIKKSLPF
                     TEEEKKWLEKHKNLIYSSDQSSPPLRYKGKEDGQYKGIIVDLINSLSIQIGRDFYFKP
                     NNWWKESFVNSIDDSIKFFDLIPSKERANKFIFTDPIYTLSANILKDKKSQDINSYMD
                     LKGKTVAIPEGDYSINFLKQKIQDINILLTPDIKTGVNHLMSGKVDAVVGDEPVLRYY
                     INNYGLSNKYSVLSNPIYTKKAVLAVPKQYEELVSILNKGIFKLQKNGVYKDLKKKWY
                     STYNEVDDILYERGIVPSIYLFIGIILISIYVFYSYTYLLKIEIKRKTEQVIENKKTL
                     EATFNSITDIIMLVDENNNIVESNKVLYDFMGEMSYKIADLISMIKGVIENTFSENTN
                     KTSEIEIHNKILKINTFPVEYKKNNTEYIVVLIKDITNDKIVEAKLLRENKMISIGQL
                     ASGVAHEIRNPLGIIRNNCYLLKDNVTMEEVNDCVKSIESNVDRASNIITNLLNFARI
                     SDDNLEHINIKNFIENIVKLQYKMLQLKNVEIKIDCEHNLICYINGESLKHVFINLIS
                     NSIDAIHQDGKIIIYCYEKNHCLFIDFKDNGEGIKEDVLKDIFNPFYTTKPIGEGTGL
                     GLYITYNEIKKNNGDISVESKLGVGTCFHIKIPLNKEVTI"
     gene            8715..10037
                     /locus_tag="ACGI59_001889"
     CDS             8715..10037
                     /locus_tag="ACGI59_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HFG9739675.1"
                     /translation="MKILLVDDELEYGVVMKKILQKKGYLVDIALSGEEAINIIKKDK
                     NYDLVLSDVMMKNMDGVQLLDRIKSINRDIEVILVTGYGSIENAVDAMKRGALSYFIK
                     SNPIENLLEEVEKVKASKISVSVQKNNFEFTLESKNRDFNNIIKIAKKAACKDVNILI
                     LGESGVGKDILARYIHSISPRKNEIFVPVNCCSFSENLLESELFGHEKGSFTGAVDSR
                     KGRFELSNKGTLFLDEIGDIPLNVQVKLLRTLEDKSIERIGSNKSIKVDFRLICAMNK
                     EPKVEISNGNIREDFFYRISTITITIPPLRKRREDLTTLIEFFLNKYQIEHDKKIHSI
                     DKEVKDFLLNYNYPGNIRELKNIINRLVVLSEEGNLSKDNLNLISNNVYIDDKISIRP
                     LREIRKEFECEYIEKVLSLCGNNISNTAKKLEISRRQLTNKISEYNIK"
     gene            10316..11602
                     /gene="grdG"
                     /locus_tag="ACGI59_001890"
     CDS             10316..11602
                     /gene="grdG"
                     /locus_tag="ACGI59_001890"
                     /EC_number="1.21.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006807454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine reductase complex component B subunit
                     alpha"
                     /protein_id="HFG9739676.1"
                     /translation="MKLELGNIFIKNVEFGEKTEVKDGVLYISSEEIEKIVLEDERIV
                     SVNVELARPGESIRIAPVKDVIEPRVKIGDESKIFPGIINKVKTVGSGRTHVLLGACV
                     VTCGNIVGFQEGVIDMSGPTAKYTPFSKTNNICIVIKAKDGIDTHDYEEAARIAGLKI
                     GAYVGEAGREVEPDEVIVYETKPLLKQVKEYPDLPTVAYVHMLQSQGLLHDTYYYGVD
                     AKQIVPTFMYPTEIMDGAIISGNCVAPCDKVTTFHHLNNPVIHDLYKRHGKDLNFIGV
                     ILTNENVFLVDKERSSDMVAKLIEFLGVDGVLVTEEGYGNPDTDLMMNCRKCSEVGAN
                     VVLITDEFPGKDGKSQSIADATKEADAVVSCGQGNLVVHFPAMEKIIGTLDYVEMMIG
                     GYKGCLNEDGSMDAELQIIIASTIANGYNHLTARYY"
     gene            11688..12995
                     /gene="grdF"
                     /locus_tag="ACGI59_001891"
     CDS             11688..12995
                     /gene="grdF"
                     /locus_tag="ACGI59_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_082007859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:12735..12737,aa:Sec)
                     /transl_table=11
                     /product="sarcosine reductase complex component B subunit
                     beta"
                     /protein_id="HFG9739677.1"
                     /translation="MSKIRVVHYINNFFAGIGGEEKADIPPEKRAGVVGPGIAFQSQF
                     NDDAEIISTVICGDTYFGENIESATQELLKMIKEESPDLFIAGPAFNAGRYGVACGTI
                     CKAVEEELNIPVITGMYKENPGVDMFKLDLHIVSTGNSAASLRKVVPIMTKLGLKLVR
                     GEEIGPPEEEGYIMRGIRKNFFHELRGSERAIDMLVKKMKGESFETEYPMPEFDRVTP
                     MKPVKDLSKIKLALVTSGGIVPIDNPDKIESSSATKYGIYDLTDMDSMSNKEFTTIHG
                     GYDRAYVLENPNLVVPLDVVRELEKEGVIGELVDYFITTTGTGTSVGNSKRFGEEFSK
                     KLLEDDVDAVILTSTUGTCTRCGATMVKEIERAGIAVVHICTVVPISLTIGANRIVPA
                     VGIPYPLGNPMLGEEESKKIRKNIVLKALNALTEDIEEQTVFE"
     gene            13287..14003
                     /locus_tag="ACGI59_001892"
     CDS             13287..14003
                     /locus_tag="ACGI59_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFG9739678.1"
                     /translation="MYILISIAICDDESYMLNHLKENIELYMKQKNLLYNIELFNKVE
                     DLLCNHEKFNIVFLDIQMEEINGMQAARKLRSYGNECFIIFVTVLNEFVFDAFEVDAI
                     NYLIKPLSNEKLYKTLDKIVTKLDDNESNYITIQKGYSYWKLKLADILYCEVIKRKIY
                     VHLKKETVDYYETIENLERSLPTNFFRCHRSYIINLQYVYGYENGNAVMESGDRIPVS
                     RLRQQIFSKVMLEFMKNRRI"
     gene            14005..15363
                     /locus_tag="ACGI59_001893"
     CDS             14005..15363
                     /locus_tag="ACGI59_001893"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFG9739679.1"
                     /translation="MINLFSSDLFLKYSFFFSFIVYRILMVIVQYYFLINFTNFNSKF
                     WCFILYSIISIFLIIINTYFNLYNLFSSQILFFLMTFLFVKYIIKQSTVLSIVSTLLV
                     YLIEQLVYGFTAPLNYLIFTSDINTDIYKFDINMGLSSLITIIIAGFIYWYSSKKFNI
                     KVMNFDKYIAILMIPLLLIILFMQSFEYSSNINIDTSLGIVKLLLNTSITEEIQAFLF
                     SIIGTICVFFSLFTFKKLIQALEDDKERAIMNQQIHAQKNYIEEAKSRLSQTISFRHD
                     FNNHLAIVNGLLKKDQILKAQDYLNKLEKVTSDLSFSCNTQNIVIDALFNNKLSIAKQ
                     LGIEVDCEVIIPQSTNMNDFDLCIVFANAIDNAIKACRVVDKQNRYLKLSTKLEGGFF
                     MIEIKNSYNPINNYTSGSGIGLLNIKEIAKKYQGAISIDKTSDYFQINVLLVISLHCA
                     SI"
     gene            15453..15887
                     /locus_tag="ACGI59_001894"
     CDS             15453..15887
                     /locus_tag="ACGI59_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9739680.1"
                     /translation="MKVGNINGQAPITNGLDSCQSEVQGLEQKKKALQDEIEKVRSDK
                     SIDGETAKLKIEALKKEMEEIENKIQELKGNKGKKTNKEENNVSEVNNNLLERKKEPS
                     NNENVKQLYDVFERSNVDEQWDNTYRVLQDDKNLKVKFNRPI"
     gene            complement(16542..16766)
                     /locus_tag="ACGI59_001895"
     CDS             complement(16542..16766)
                     /locus_tag="ACGI59_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HFG9739681.1"
                     /translation="MTVYNLKLGQKGIIDNIAGNEKLMKRLLALGLIDGTEVEVKKIA
                     PLNDPIVIRFRGFDLAIRKSDAKNINLKNN"
     gene            complement(16863..18116)
                     /locus_tag="ACGI59_001896"
     CDS             complement(16863..18116)
                     /locus_tag="ACGI59_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HFG9739682.1"
                     /translation="MITLSFDIVQTLTLSIFFFLIGNLLKNKVNFLNNFCIPAPVIGG
                     LLFCFLNLFFKYFNIADISISGNLMPNFITFFFTTIGLEISINLIKKGGSVLFRYWIL
                     CGVLAFCQNILAITISKIIKLEPLLGLMCGNVSMEGGHGYSAAFGLTIENLGIEGAVG
                     VGLSAATIGLIMGGILGCPVAKFLINKYKLKPSSNIDLSIPRYNRNLKRFGSKFFRNK
                     NRIKNNNFSETITPSVFLEQVLLIFICINTGEIISRCFYITFNILLPSVVTCMFSAVI
                     FRNLNDKINILELNFKLIDFLKELSLGIFLTLSLMNIDLFELSTLLPPILLIVTFQVI
                     FIILFSIFICFRVLGKDFDSAIIISGLIGHGIGATPNALANMSSLTQKYGDSPKAFLV
                     VPLVSGFLLDAISIPCILFFINILT"
     gene            18376..19365
                     /gene="add"
                     /locus_tag="ACGI59_001897"
     CDS             18376..19365
                     /gene="add"
                     /locus_tag="ACGI59_001897"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFG9739683.1"
                     /translation="MFENLPKIDLHCHLDGSVRVETMLDIAIKEKIDLPSNNMDEIKK
                     LAKVSFNCTSLDEYLEKFDLPLKVMQSKENLKRITFELLEDASRENVKYIEIRFAPLL
                     HTQKGMSVKNIIEGIIEGIREAESIYDIKGNLILGCMRTMTSKEALLVIEEGKSFVNK
                     GVVAVDLCGPEKEGFCKEYKDVFKLAREYGYKVTIHAGEAASGENVLDAINILKADRI
                     GHGVKIKDHKKAYNLVKDKKILLELCPTSNVQTKTVDSYEAHPFYTFYKDNLHVSINT
                     DNRTVSDINLSSELNVIFDTFKLGLEDYKIIYRNAVEASFADKETKEYLNSLI"
     gene            19437..20177
                     /locus_tag="ACGI59_001898"
     CDS             19437..20177
                     /locus_tag="ACGI59_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EcsC family protein"
                     /protein_id="HFG9739684.1"
                     /translation="MKDYEDVVYIKLVKKELEEWKKNILKKPSIFDKASKGIQNKFNG
                     VLPDKYHEIITSSIKVMTKTVLFGYKYATKPSLRNMSLEEREKLVLEKAKTYKTTAMI
                     EGAGTGAGGILLGLSDFPLLLGIKVKFLYDVASIYGYDVKDYRERIYILNILQLAFSS
                     QQHINKTFKLMNNWDEYVNSLPEDINDFDWRTFQQEYRDYLDLAKFLQLMPGIGAFVG
                     FYVNGKLIDKLCETAVSAYRLRIRELKL"
     gene            20377..21528
                     /locus_tag="ACGI59_001899"
     CDS             20377..21528
                     /locus_tag="ACGI59_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double-cubane-cluster-containing anaerobic
                     reductase"
                     /protein_id="HFG9739685.1"
                     /translation="MTNLPKQFDSFNEARQKGFINAKELKESGKKMVGVFCTFTPVEI
                     PMAAGATVVGVCGVSEEPIPDAERVLPRNLCPLIKSSYGHAITDTCPYFYFSDLLIGE
                     TTCDGKKKMYEELAKVKPTYVMHLPNTSKGEFAYKLWKDEMIRLKEEVEKSLGVTITE
                     EDIRTAIKDKNEERELLKEFYALGKLQPSALTGLELHNVLYQAGFKFDRAELKHSLRK
                     VIDDMKERYEKGECPVQKDKPRILITGSPIGGISEKIVKTLEDAGASVVAYELCGAIR
                     SNDLLVDEEIEDVYDALTQKYINIGCSCMMNNDNRIELLDRIIDEYNVDAVIDVVLQA
                     CHTFNIESYRIREFVTKEKNKPFMSLETDYSKSDTEQLRTRFEAFVEML"
     gene            21553..22308
                     /locus_tag="ACGI59_001900"
     CDS             21553..22308
                     /locus_tag="ACGI59_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HFG9739686.1"
                     /translation="MFSIGVDSGSVATKGVLFDGEKIIKKIIIPTGWSPKSTSKQVYE
                     LLSSEIDKKDIKKVVGTGYGRGVMDFADKKVTEITCHTRGIYFLNKNIRTILDVGGQD
                     SKVINLDRDGNVFNFIMNDKCAAGTGRFLEITSNLLGSDIESIDTLAKGYEPVNISSM
                     CTVFAESEIVSLLAQNISTGEVAAGILKSIANKSTSMLARGEVIDEVAFTGGLAKSKE
                     LVKMIEEILGKKIFIAEDTQIIGALGAAVIGFR"
     gene            22500..24887
                     /locus_tag="ACGI59_001901"
     CDS             22500..24887
                     /locus_tag="ACGI59_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding cysteine protease Cwp13"
                     /protein_id="HFG9739687.1"
                     /translation="MKKFTSKKVTRFVALFLISILVMSTIPVSADNSNTLNQIKKAEY
                     SETYKQYLNDAKNGRTEKYNGIIPNPYQLEGTQIQSKGRTAPTSYDPRKLGLMTSIKN
                     QEDLGICWDFAAMATLESFLKLNNYGDYDLSEEHLRWWASDGEYGWSVNDMNGALNYE
                     AMGYLTSWSGPKLEKDIPYNGRVSKAQGAKKPTNMNTAPTVFNVTDAVCVSNDINSTK
                     NAILQYGAVTSGYYEDIKYQSDDQNSYYCPTKSFNTNHAISIVGWDDNYSKDNFNANI
                     RPSKNGAWLIKNSWGDYNSEGGYFWISYEDKTLMSDIDNFSIKGGKKPNDDEKMYQHD
                     YASIVPLISKKITAANVFDFNRGDETLKSVMFLTESIGAKYEVYYAPVVNGIPQENNM
                     KKLKEGTAQYSGYITVPIDSFDIPEGKGAIVVSIEGKNGESTIGSESNVPGYDTFKAK
                     ANLGESYIIDNTGKFFDINRDSNFYPCNFTIKAVTEKSSGESIPNESLIGSDRYETAI
                     KVSQNGFNSSENVVLVNGSSIVDALAATPFTSAINSPILLTQKEALNSKTKAEIQRLG
                     AKKVYLIGGENSISKEIEQQLKSLNISIERISGSDRYKTSLLLAQKLNGIKNVSQIAV
                     VNGVKGLADAISVGAAAAENNIPIILANEKSELQGADEFLNSLNIEKSYIIGGTASLS
                     NNLESKLKNPTRLSGSSRDETNSKIIDNFYKKDTLKNAFVVKNGIKNQNDLIDGLAVG
                     PLGAKTGSPVILVGDKLADSQKEVLKNKTLEKVTQVGGGANKNAFKELVKLKSAK"
     gene            complement(25118..25351)
                     /locus_tag="ACGI59_001902"
     CDS             complement(25118..25351)
                     /locus_tag="ACGI59_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFG9739688.1"
                     /translation="MATTTKNRFYDYVQENLNKYVEVHSYFSSCPLYGEIIEADSLSI
                     TLCSRYIDESNPTLNETYTLYLPLSSIISIRTL"
BASE COUNT        10200 a         2655 c         4276 g         8313 t
ORIGIN      
        1 aaaagtgatt atttcaattt agttatagaa cttagtcata taaatatata gaaaatattt
       61 taatatgtta tacataacgg gttattttat aagtttaact ataaattagg tttatatttt
      121 ataaaaattt gctaaaaaaa acaattaaaa attgtaaaga aaaaagatat aattagaact
      181 gtagtaataa atgttttatt atagataata gagttttaag tatcttaaat aatatttgga
      241 ggaatatgaa tggaattaag accaagaagt gcaaaattag aagaatttga taaagtaata
      301 gaattaataa actatgtatt tagaatatca agaaatcata aaccaactat gatggaagaa
      361 tttcctcttt tactaagtaa aaataatata gaaaatatga ttataatatc agaagatgat
      421 aaagtgattt ctgatgtaaa ctatctaatt caggatgtat ctattcaagg aaacagattg
      481 aaggttgcag ctataggtgg agtatgtact catccagatt atgaaaagag aggctattca
      541 tctaagatat tggataaagt agaagaaaaa atgttttatg atggtgtaga tattgtaatt
      601 atatcaggaa caagaagtct atacagtaga agaaattgta gtttggtaaa gagtttttat
      661 aaatatacaa taaaacctga agatgtaaaa atagcatatg aaatagttga atttgatgaa
      721 actaattttg aaaaagataa tgatttagat aaaatgattg aactatataa tcaaaatagt
      781 actagattta taagaacgag agatgagttt caaaaactat tgcatgcagc aactattgcc
      841 tggggaccaa ttggttacaa gaaggtattt ataaaggaaa ataataatat aataggatat
      901 ttgataataa gaacaataaa aaaagaagat tctactgtag gagaagttgc tgaaataggg
      961 ttaaactctg taaatgttga aaatatattg aaatatgttg caaataaatt tggattagaa
     1021 tatttaaatt ataaagtaca tgtgaaaaat cttaaagacc agttgaaatg taatgggaca
     1081 aaatctttgg attatcaaca aggaactatg aaaataataa attttactaa gttgtgtgat
     1141 agtttaagaa gttattttag tcaatatgta gattttgaat tactaaaata catggaattt
     1201 aaacaagttg aaaataaata tattattaaa tacaaagaag aagaacttgt aatagaaaat
     1261 ttggataagc ttaataaact gttttttgaa aaaaatgagg aacagtacaa tgaatttaaa
     1321 catctaaaaa atatatatga atttgctaca aaggcatttc ctgtagattt tccatggact
     1381 gcaaatttaa attatcaata gttttagatg atattaaata aaattgattg atacctagag
     1441 ttagtccttg ttaaataaga gaaattattg gatattgaaa aagcaatttt gtaagtaatt
     1501 agaaaggatg agtattatat ggacatactt gatgatatag cagaatgtat attaaatatg
     1561 ggtgtagata atatagagaa tttagtaaaa attgctatag ataaaaatat tgatgtggaa
     1621 gatatttatg aatatggttt aaacaaaggt atgataggag ctttagataa gtttgagaat
     1681 aaggaatact atctttcaga agttatagta tgcacagatg cattaaataa aggtattaat
     1741 cttttgaagg gaactggaaa agtaaagaaa aaatcaaagg gagtaattct gatgtctgta
     1801 gtagaaggag atactcatga gataggaaaa aatattgtaa aagtaatggt agaagccact
     1861 ggatataagg ttattgactt aggtgtaaat agaaaaagtg aagatataat agaagaagct
     1921 ataaaaaaca atgtagatat aataggctta tcttcaatga tgacaactac aatggaaaat
     1981 atgaaatcgg ttatagacga acttaatatg attgagatag ataaaagacc aaaagtaata
     2041 attggtggag gacctgtaag tatggagttt gcagaagaaa taggagcaga tggatacagt
     2101 tcaaatgctc caaaagcagt taaattaata aataaactta taggaggtga agtttaaatg
     2161 ttttcagata tgactttact tcagtatata aatagtgaag atagaggttt ttttctgcct
     2221 gatatgggta caaatggatt gttcttaata aataaaaagg catatgaagt atatgatagt
     2281 gtagatttgc aattagaatt agcaaagact atggacaagt attttgaatc agattttata
     2341 tactcattct gtgatgggat tacattttgt gaaactctgg gtttagagac attaagacca
     2401 gattatgatt ttccaagtgt attgacacat actataactg acagagataa attaagaaaa
     2461 ttagatgttc cagaccctta tacgagtgga cgtatgccac tcaatataga gagcttatca
     2521 gttatatcaa aaagtattaa aaaaccacta tatgaatcga tacaaggtcc atttacactt
     2581 gcaggtcaat tagctggagc tactcaactt ttaagatgta taataactga taaaaaattt
     2641 gtagaagaat tattggaatt tacaactgaa ttggtaagaa gatatgctgt agctgcaaat
     2701 aaagcaggag caaaatatat ttcaatagca gagccaacat cagtaaccct aagtaaagat
     2761 agatttgatg aatttatagt aaaaaattta aataaaatct acaatgaact tgattgttgg
     2821 aaaggtatgc atatatgtgg agacaccaga gaattattag ataatatgct aagttgtaat
     2881 atagatgcag taagtttaga ccaaatttta gactatgaag aaattgctcc aatgattcct
     2941 agagatatag ttttgattgg taatttagac ccaataaaac ttcttggacg ttctaagcca
     3001 gataagatta gaagagagac gctaaaattg ttaaagaaga tgagaagata tgataacttt
     3061 ttatgtgatt ttggctgtaa ctgcttaaat accactccag tagaaaattt acaagcagct
     3121 ataaaggcag gaagaattag ttataaagaa ttagatagga ttgatattga tgaattataa
     3181 tagtaataaa aataaatatc ttttaaagga tataaattta gatgatattt ttatagataa
     3241 gaatattgtg ctaaagtttt tggggtatgg aaatagaaaa gctccagata caattagtaa
     3301 aatcgttgaa agagaaataa gaaatataaa tgatatttta gatatcaaga tatatataag
     3361 tgaagttgat attaataaaa cacctaaaga atgtaaaaaa gcatttgctg tactatatac
     3421 aataggggat aaaattgata ttaagatgaa tgactatatg gaaaattgca atatgatggc
     3481 agggctagca ttggataaaa taggtatagt atgtttagat tatattaatg agaaaataaa
     3541 aatgtattta aaaggaaagt atgatagtct caaaatatcg catgaaatat atccaggaga
     3601 taaagatttt gaagtagaga gacaaaagga tatttataac tatgtaaaaa ataaatataa
     3661 tagtatagaa atagaaatta atgattatca tcaactatcc cccataaaaa gtgttgcaat
     3721 gcttatttta atgggggatg aagaaaattt agaatcaaga tgtagtaagt gtcctagaaa
     3781 atgcttttag ttaattttca agactaagat ttgttttgtg tataaattgc aataattttt
     3841 ttggtcattt tatgtataaa ctaaaaaaat tattgcaaag taatattcta tttaatttga
     3901 acagacttac aaactatagt agtttcttta attcaataag tgtatttatt tcataagttg
     3961 gtatatgatt agtataattt atactgttat tggaatttaa ccaacaagta tctattcctg
     4021 aatttattcc acctaagaca tcagaggtta agctatcccc aattattaag gcagaagatt
     4081 tatcatgaca attaaacttt ttgagagcgt atttaaatat ttcaggattt ggttttgata
     4141 ttttcaactc ttcagataca accattccat caatatatcc ttttattttt gaattttcaa
     4201 gtcttttatt ttgaactgca gaaattccat ttgttattat aacaatagta tacttttttt
     4261 taagatattg taatatatcg attgcacctg gaattaaaaa tgtacattca cctaaaaaat
     4321 ttaagtacat tttgctaaga gtttcagaat caacatccaa agatatttta tttgcaaaaa
     4381 gttcaaatct aagtaattta agttcattca aagttataat atttttttca aggtcgagcc
     4441 aaagcttatc acttatattt ctatatgact ctatataatt ctcaaaatta aattctaaat
     4501 caaactcaga tagaagtttt ttaaaagcat gtgattcact tttcttaaaa tcaaataaag
     4561 tatcatctgc atcaaaaaat ataaatttat atgtcttcat ggtttttact cctttacaag
     4621 atgactgcaa aaattacttt actttcacta tttgagtcat tttgccattt atgttttgtt
     4681 cctaaaggaa cttttacact atctcctttg gacaaagtaa attcatcatc atccataaaa
     4741 agtttaactt ctccctccaa aacatatgca atttcatctc cattatgagt aatcctatca
     4801 acacaagaag aagtgtttgg agctaaatcc ataattgcaa attctaaatt accactagag
     4861 tctggaatta aaatctcata tataacttct ttgcttccag gtaaaataac tttttttcta
     4921 ttatcatgtc ttactatcaa gtttttttta tctacttcag atacaaaaaa tgtaaataaa
     4981 ggaacattta gagaagaggc tatagatttt aatgaattta cagatggatt agcaagacct
     5041 cgttctattt ggctcaataa tgatggtgta acatcagcta gttttgctaa atctcgaata
     5101 cttaagtttt gtttttttcg aacttcagct attttttccc ctaaatttaa atcattcata
     5161 taaatatcct ctctttttaa tttaaaaaat attaacttaa aataatcaag taaaatgatt
     5221 atgtagtatg aaattattat aacatataga attaggtttt gaaaaaacta tgttcaatat
     5281 aattaaataa attaaaatat aattgacaaa gtggatacag aatacttaaa atatatttaa
     5341 ataaaattaa gcaaaagata gttaatttat gataggaggg gatattatgc aaataacgac
     5401 ttttggagct atatttggtt tattaatagc aattatttta attataaaaa aatttcaagc
     5461 tgtatatagt ttaatgttag gagcatttat tggaggatta gttggaggag caaatataac
     5521 acaaactgta gattttatgg caaatggagc tatgaatata tcaccatcaa ttcttagagc
     5581 tttggcatca ggtgtattag ctggaagttt aataaaaaca ggggcagtag ataaaatatc
     5641 agaacagatt gtaaaaatat ttggtgaaaa gagggcttta ttttcgattg ccatttcaac
     5701 tatggtactt gctggagtag gtgtaaattt ggatgtatct ataataactg tagcacctat
     5761 aggattatat ataggtagaa aattaaatta ttctaaattg tcaatactat tagctatgtt
     5821 gggagggggt aaagcaggta atataatatc accaaatcca aatactatag cagtagcaga
     5881 taatttttct gtcaatctct ctagtgttat gatggcaaat ataattccag caattattgg
     5941 agttgttatt acagttattt tagcaagtat attaataaat aaaggaaata aagtacaatc
     6001 atatgaaatt ttagagcaaa gagaagattt accaagttta tttaaatcat tatgtggtcc
     6061 aattatagct atatttctat tgtttttagg aaatgtatct cctatagtta tagacccaat
     6121 gatagccctt ccaataggag gaatcgtaac tctaattgtc acaggaaact tgaacaattc
     6181 aagagaatat ttaacttttg gattatctaa gatgcaaggg gtatgcatat tattacttgg
     6241 aacagggact atcgcaggaa ttatacagat gtctgaatta caacagagta caataggtgc
     6301 tttacaattt ttaaatatgc cacaattttt attggcacca gtttcgggta tacttatgtc
     6361 attagcaaca gcatcatcaa cagctggtgc tactatagca tcatctactt ttcatgatgc
     6421 tataataaat ggtgggctat caccgatttc aggtgcatca atagtaaatg ctgggtctag
     6481 tgtattcgag caattaccac atggctcact atttcatact agtgcaggta gtataaacat
     6541 ggatataggt gaaagattta agttaattcc atatgaagca ttgataggaa tagtaatgac
     6601 tataatttca acatctattc aattagtttt ataataaata taaatattta atgaaaaaag
     6661 taggattttg ttattaaata ttgaacttat tatagtaaaa aagaagattg gagttttaaa
     6721 gtgaataaaa aaaagattgt tattataggg attatttatt catttttagt agtattttca
     6781 cttacaaata tgtatgtaaa tatggagtat aatctaaatg tatttgaata tattaagaag
     6841 tcacttccat ttacagaaga agaaaaaaag tggttagaaa aacacaagaa tttgatttac
     6901 agctctgacc aaagttcacc tcctttgaga tataaaggta aggaagatgg acagtataag
     6961 ggaattattg ttgatttaat aaattctctg tcaattcaaa ttggtagaga tttttatttt
     7021 aaaccaaata attggtggaa agaatctttc gtaaattcaa tagatgatag tataaagttt
     7081 tttgacttga ttccatcaaa agaaagggca aataaattta tatttactga cccaatatac
     7141 actttgagtg caaatatatt gaaagataaa aagtcccaag atataaatag ttacatggat
     7201 ttaaagggga aaacagtagc aattccagaa ggtgattatt ctataaattt tttaaagcaa
     7261 aaaattcaag atataaacat tttattgact ccagatataa agactggagt caaccattta
     7321 atgtctggaa aagtagacgc agttgttggt gatgaaccag tattgcgata ctatataaat
     7381 aattatggac tttcaaataa atacagtgtt ttaagtaatc ctatatatac taaaaaagca
     7441 gttcttgctg tacctaaaca atatgaagag ctggttagta ttttaaataa aggcattttt
     7501 aaacttcaga aaaatggagt gtataaagat ttaaagaaaa aatggtattc tacgtataat
     7561 gaagttgatg atatattgta tgagcgagga attgtgccta gtatttatct ttttataggt
     7621 ataattttaa tatcaattta tgttttttat agctatacat atttgttaaa gatagaaatc
     7681 aaaaggaaaa cagaacaggt aatagaaaat aaaaaaactt tagaggcaac ttttaatagt
     7741 attacagata taataatgct tgtagatgaa aataacaata tagtagaatc gaacaaagta
     7801 ctatatgact ttatgggtga aatgagttat aagatagcag atttgattag catgataaaa
     7861 ggtgttattg aaaatacctt ctctgaaaat actaataaaa ctagtgaaat agaaatacac
     7921 aataaaattt taaagataaa cacttttcct gtagagtata aaaaaaataa tactgagtat
     7981 atagtagttc ttattaaaga tataactaat gataaaatag tagaggcgaa attacttaga
     8041 gaaaacaaaa tgatatctat agggcaatta gcttctgggg tagcacatga gataagaaat
     8101 cctttgggta taataagaaa taattgctat ctcttaaaag ataacgttac aatggaagaa
     8161 gtaaatgatt gtgttaaatc aatagaaagt aatgtagata gggcaagtaa tataataacc
     8221 aacttgttaa atttcgcaag aatatcagat gataatttag aacatataaa tataaaaaat
     8281 tttattgaaa atatagtgaa acttcaatat aaaatgttac agttaaagaa tgttgaaata
     8341 aaaattgact gtgaacataa tttaatatgc tatataaatg gagaatcgtt aaaacatgta
     8401 tttataaatt taatttctaa ttcaatagat gcaatacatc aagatggaaa aattattata
     8461 tactgttatg aaaaaaatca ttgtttattt atagatttta aagataatgg tgaaggaata
     8521 aaagaagatg tcctaaagga catatttaat cccttctata caacaaaacc aattggtgag
     8581 ggaactggtt taggtttata tattacttat aatgagatta aaaagaataa tggagatata
     8641 agtgttgaaa gtaaattggg ggttggaact tgttttcata taaaaattcc attaaataaa
     8701 gaggtgacta tataatgaaa atactattag ttgatgatga gctagaatat ggagtggtaa
     8761 tgaaaaaaat tctacaaaag aagggctatc ttgtagatat agcactaagt ggtgaagaag
     8821 ctataaatat aattaaaaaa gataaaaatt atgatttggt tttatctgat gttatgatga
     8881 aaaatatgga tggagtacag ctattagata gaattaaatc tattaataga gatatagagg
     8941 ttatattagt tacaggatat ggaagtattg aaaatgcagt tgatgctatg aaaagaggag
     9001 ctttatctta ttttataaaa agtaatccca ttgaaaattt attagaagaa gttgaaaagg
     9061 taaaggcaag taaaatatct gtttctgtac aaaaaaataa ttttgaattt actttggaat
     9121 ctaaaaatag agattttaat aatatcataa aaatagcaaa aaaagctgct tgtaaagatg
     9181 ttaatatatt aatcttgggg gaatctggtg taggaaaaga tatattagct agatatatac
     9241 atagcataag tccaagaaag aatgagatat ttgtacctgt aaattgttgt tctttttcag
     9301 aaaatctatt ggagtctgag ctgtttggtc atgaaaaagg ttcttttaca ggagcagtag
     9361 atagtagaaa aggtagattt gaactttcga ataaaggaac tctatttttg gatgaaatag
     9421 gagatatacc tctaaatgtt caggtaaaac ttttgaggac acttgaggac aaatctatag
     9481 agaggatagg aagtaataaa tctattaaag ttgactttag gttaatttgt gctatgaata
     9541 aagaaccaaa agtggaaata tcaaatggaa atataagaga agattttttt tatagaataa
     9601 gtacaataac aataactata ccaccactaa gaaagagaag agaagactta actactctta
     9661 tagaattttt cttaaataag taccaaattg aacatgataa aaagatacac tcgattgata
     9721 aggaagtaaa ggatttttta ttaaattaca attatccagg aaatataaga gaattaaaaa
     9781 atataataaa tagacttgta gttttaagtg aagaaggtaa tttatcaaaa gacaatttaa
     9841 atttaatatc aaataatgta tacatagatg ataaaataag tattagacct ttaagagaaa
     9901 taagaaaaga gtttgaatgt gagtatattg aaaaagtttt atctttgtgt ggaaataata
     9961 tttcaaatac agctaaaaaa ttggagatta gtagaagaca gttaaccaat aaaatatcag
    10021 agtataatat taagtgagaa aaaaacttct cattaagtga gaaaaaaatt tctcaaactg
    10081 tattaaaaga taaacagaaa aaatctgttt atcttttttt attataattt taaaaattaa
    10141 tatttacaat aattattgct aaattcaata ttggtgtaaa ttaaaaatga aaatttctat
    10201 gacattaata catagaatat atattttaaa taattggcat agaaaatgct ttatatatta
    10261 ataaacgtaa taaatacttt taattgcgat taaatatatt aagggggatt ttcatatgaa
    10321 gctagagcta gggaatatat ttataaaaaa tgttgaattt ggagaaaaga cagaagtaaa
    10381 agatggagta ctatatatta gtagcgaaga aatagaaaag atagtattag aagatgaaag
    10441 gattgtaagt gtaaatgttg aacttgcaag acctggcgaa tcaataagaa tagctccagt
    10501 taaagatgta atagagccta gagttaaaat tggagatgaa agcaaaatat ttcctggaat
    10561 tataaataaa gtgaagactg taggaagtgg aagaacccat gtacttttag gagcttgtgt
    10621 tgtaacttgt ggaaatatag ttggatttca agaaggcgta attgatatga gtggtcctac
    10681 tgctaaatat acaccttttt caaaaacaaa taatatatgt attgttataa aagcaaagga
    10741 tggtatagat actcatgatt atgaagaagc agctagaatt gcaggattaa aaataggtgc
    10801 atatgtaggt gaggcaggaa gagaagttga gccagatgaa gtcattgtat atgagacaaa
    10861 gccattatta aaacaagtaa aagaatatcc agacctgcca acagtagcat atgttcacat
    10921 gttacaatca caaggacttt tacatgacac ttattactat ggggtggatg ctaaacagat
    10981 agtgcctaca tttatgtatc caactgaaat aatggatgga gctataatat ctggaaactg
    11041 tgtagcacca tgtgataagg ttacaacatt ccatcattta aataatccag ttatacatga
    11101 cttatataaa agacatggaa aagatttaaa ctttatagga gttattttga ctaatgagaa
    11161 cgtattctta gtcgataaag agagaagctc tgatatggta gctaaattaa tagaattttt
    11221 aggagttgat ggagttctag tgacagaaga aggttatggg aatccagata ctgacctaat
    11281 gatgaattgt agaaaatgta gtgaagttgg agcaaatgta gttttgataa cggatgaatt
    11341 tcctggaaaa gatggcaaga gtcaatctat agcagacgct actaaggaag cagatgcagt
    11401 tgtttcttgt ggtcaaggaa atctagtggt tcatttccca gcaatggaaa aaattatagg
    11461 aactttagat tatgttgaaa tgatgatagg tgggtataaa ggatgcttaa atgaagacgg
    11521 aagcatggat gcagagctac aaattataat agcatcaact atagcaaatg gctataatca
    11581 tttgacagca agatattatt aaataagtat ttgaatataa agttctgttg atgtaatcat
    11641 tggtctaact gatgtaataa ctataaagtg atttggagga ttaagagatg agtaaaatca
    11701 gagtggttca ttatataaac aatttctttg ctggtatagg tggagaggaa aaagcagata
    11761 ttccgccaga aaaaagggct ggtgtagtag gacctggaat agcatttcag agtcaattta
    11821 atgatgatgc agagataatc tcaactgtaa tttgcggaga tacgtatttt ggtgaaaata
    11881 ttgagagtgc aactcaagaa cttttaaaaa tgataaaaga agaaagccct gatttattta
    11941 ttgcaggccc agcatttaat gcaggtagat atggagtagc ttgtgggact atttgtaagg
    12001 cagttgaaga agagttaaat attcctgtaa ttacaggcat gtataaagaa aatccaggtg
    12061 tagatatgtt taaattagat ttgcatatag tgtctacagg aaattcagca gcaagtctta
    12121 gaaaggtagt tccaataatg acgaaactag gtcttaaatt ggttagaggt gaggaaatag
    12181 gacctcctga agaggaagga tatataatga gaggaataag aaaaaatttc ttccatgaac
    12241 ttagagggtc tgaaagagca attgatatgt tggttaaaaa gatgaaaggg gaaagctttg
    12301 aaactgagta tccaatgcct gaatttgata gagttacacc tatgaaacct gttaaagatt
    12361 tatctaagat aaaacttgcc ctagtaactt ctggagggat agttccaatt gataatccag
    12421 acaaaattga atcttcaagt gctactaaat atggaattta cgatttaaca gatatggatt
    12481 ctatgtcaaa taaagaattt acaactatac atggggggta tgacagagca tatgtccttg
    12541 aaaatccaaa tttagttgta ccactagatg tagttagaga gcttgaaaaa gaaggtgtaa
    12601 ttggagaatt agtagattat tttataacta ctacaggaac aggaacgagt gttggcaatt
    12661 ctaagagatt tggagaagag ttttctaaga aattgttaga agatgatgta gatgctgtaa
    12721 ttttaacttc tacatgaggt acttgtactc gttgtggagc aacgatggta aaagagattg
    12781 agagagctgg tatagcagtt gttcatatat gtacagtagt accaatatca cttacaatag
    12841 gagctaatag aattgttcca gcagtaggga taccatatcc acttggaaat cctatgttag
    12901 gagaagagga aagtaaaaaa attagaaaga atatagtttt aaaagcttta aatgcactaa
    12961 cagaagatat agaagaacaa acagtatttg aataataaaa atacaacaca acactgaaat
    13021 aaaaaaagat atcaaaaaat aattttttaa aattatttta cgatatcttt ttttattagg
    13081 taaattcgaa atttgcattt ttttaaatta aatttataaa agtgataaat tagtttatta
    13141 taatattgat acacaaattc aaattagata tactatgaaa agcgagatta ttacattttg
    13201 gaacaaatat tcaacatatt agatcatatt aattgaacag aaaaaattaa ctttatataa
    13261 taattagaat aatgataaga aagaaggtgt atatattgat ttcaattgcc atttgtgatg
    13321 atgaatccta tatgctaaat catttaaagg aaaatataga attatacatg aagcagaaga
    13381 atttattata taatatagag ctttttaata aagtagaaga tttactatgc aatcatgaaa
    13441 agtttaatat tgtattttta gatatacaga tggaagagat taacggaatg caggcagcta
    13501 gaaaattaag gtcatatggt aatgagtgct ttataatttt cgttacagtt ttaaatgaat
    13561 ttgtatttga tgcttttgaa gtagatgcta taaattatct gataaaacct ctatctaatg
    13621 agaaactata taaaactttg gataaaatag ttacaaaact tgatgataat gaaagtaatt
    13681 atatcacaat acaaaaaggt tattcatatt ggaaattaaa acttgctgat attttatatt
    13741 gtgaagttat aaaaagaaag atttatgttc atttaaagaa agagactgta gattattatg
    13801 agactattga aaatttagag agaagtttac caacaaattt ttttaggtgt catcgtagtt
    13861 atataatcaa tttacaatat gtatatggat atgaaaatgg taatgcagtt atggaaagtg
    13921 gagatagaat acctgtttct agactacgtc aacaaatatt ttcaaaagtt atgcttgagt
    13981 tcatgaaaaa taggaggata taagatgatt aatttatttt cttccgattt atttttaaag
    14041 tacagttttt tcttctcttt tattgtatat agaattttaa tggtaatagt gcaatactat
    14101 tttctaatca attttacaaa ttttaattca aaattttggt gttttatttt atattcgata
    14161 atttcaattt ttttaatcat tataaataca tattttaatt tatataattt attttcttca
    14221 cagattctat tttttcttat gactttttta tttgtaaagt acataataaa acaatctact
    14281 gttttatcta tagtatcaac tttgttagta tatttaattg aacaattagt atatggattt
    14341 acagctccat taaattactt gatatttact agtgatataa atacagatat ttataaattt
    14401 gatataaata tgggactatc ttctttaatt actattataa tagctggatt tatatattgg
    14461 tatagttcta aaaagtttaa tattaaggtc atgaactttg ataaatatat agcaattttg
    14521 atgattccat tgttgcttat aatattattt atgcaatcat ttgaatatag ttcaaatata
    14581 aatatagata caagtcttgg gattgtaaag ctgctattaa atacttctat aactgaagaa
    14641 attcaagctt ttcttttttc tataattgga actatatgtg tatttttttc tctatttacc
    14701 tttaagaaac taattcaagc tctcgaagat gacaaggaga gggcaattat gaatcaacag
    14761 attcatgctc aaaaaaacta tattgaagaa gcgaaatcac gattatcaca aactatatcc
    14821 tttagacatg attttaataa tcatttagct attgtcaatg gattgttgaa aaaagaccaa
    14881 attttaaaag cacaagatta tttaaataag cttgaaaaag taacaagtga cctatcattt
    14941 tcttgtaata cacagaatat tgttattgat gctcttttca acaataaatt aagtatagca
    15001 aaacaattag gcatagaggt agattgtgaa gtaattatac cccaaagcac taacatgaac
    15061 gattttgacc tatgtatagt gtttgctaat gctattgaca atgcaattaa agcttgtaga
    15121 gttgtagata aacaaaatag gtatttaaag ctttccacta agttagaggg tggatttttt
    15181 atgatagaaa taaaaaatag ctataatcca ataaataatt atacaagtgg ctcaggtata
    15241 ggtcttttaa atataaaaga aattgctaaa aaatatcaag gggctataag tatagataag
    15301 acatcagact attttcaaat aaatgtattg ctagttattt cattacattg cgctagtatt
    15361 tgagaacaat ttcattgaaa atagtatttg taagctatat tatgtatttg aaaagagaaa
    15421 gtttgatata tagaaatgag gaggttacta aaatgaaagt tggaaatata aatggtcaag
    15481 caccaattac aaatggttta gatagttgtc aaagtgaggt acaaggctta gaacaaaaga
    15541 aaaaagcatt acaggatgaa atagagaaag taagaagtga taaaagtatt gatggtgaaa
    15601 cggccaaatt aaaaatagaa gctttaaaaa aagaaatgga agagattgaa aataaaattc
    15661 aagagttaaa aggaaataaa ggaaaaaaaa caaataaaga agaaaataac gtttcagaag
    15721 taaataataa tctattagag agaaaaaaag aaccttctaa taatgaaaat gtaaaacaat
    15781 tatatgatgt ttttgaaaga agcaatgtag acgaacaatg ggataatact tatcgtgttc
    15841 tccaagatga taaaaactta aaagtaaaat ttaatagacc tatatagttt atttataaaa
    15901 atagttaata ttttaattat caccactttt tagaagaata cacactttat tttaaagacc
    15961 cacatattta tttacagtta gcacatttat atgtggtttt ataactatta tttacaagtc
    16021 tatattgata acttttacat agtatatgat gtttaaatac ttattcaaat ttatctatac
    16081 ataaacaaaa aaagagattt taaaatctct ttttagattg ttgacttatg gctttttgat
    16141 ttaataaaat caagaagccg tttgaatttt atatgttggc tgataccctt ctgatattga
    16201 aacaccattt ttgtttccaa aatcgtttaa aattaagaac ttattatgaa tgactatttc
    16261 attatgaaca ataagcaaat aacatgaatc ttcaatcaat gttttaacag tagcattaaa
    16321 tgcttgctta tatagttcta tcgtttgttt gaactgccca ttaaagtaaa tatgagtcaa
    16381 taacataact atatctacac aaattaatat aaatttttta tactgtacat tatctaactg
    16441 ttgattatct gtattacaac atgattgaat ttaaaaagcc atcaacacat agaatgttga
    16501 tggcctgaaa atagatttta aaatctcttt tttattatat attaattatt ttttaaattt
    16561 atattttttg catcagattt tctaatagct aagtcaaacc ctctaaatct aatcactata
    16621 gggtcattaa gaggagctat ctttttcact tcaacttcag tgccatctat tagtcctaag
    16681 gctagaagtc tcttcataag tttttcattt ccagctatat tgtctataat tcccttttgt
    16741 ccaagtttta agttgtaaac agtcattatt aacacctcct aatgaaaatc attatcaaac
    16801 tttatattta taatatataa caaaaagagc aaattgtcaa tatataacaa aaaataaaca
    16861 tactaagtta gtatgtttat aaaaaatagt atacatggga tactaatagc atctagcaaa
    16921 aaaccactta ctaatggtac aactaaaaag gcttttggag aatcaccata tttttgtgtc
    16981 aaagaactca tatttgccag agcgttcgga gtagctccta ttccatgtcc aatcaaacca
    17041 cttattatga tggcagaatc aaaatctttt ccaagtactc taaagcaaat aaatattgaa
    17101 aataggatta taaagattac ttgaaaagtt actattaaga gaataggagg taataatgta
    17161 gataattcaa ataaatctat attcataagt gataaagtta aaaaaattcc caatgaaagt
    17221 tcttttaaaa aatctataag tttaaaattt aattctagta tatttatctt atcatttaaa
    17281 tttctaaaaa taacagccga aaacatacaa gttacaacag agggtaatag tatattaaac
    17341 gttatataga agcatctact tataatttct cctgtattta tacaaataaa tatgagaaga
    17401 acctgttcca aaaatacaga tggtgttatt gtttcggaga agttattatt ttttatccta
    17461 ttcttatttc taaaaaattt acttccaaat ctctttagat ttctattata tctaggaatc
    17521 gacaagtcta tatttgatga tggttttaat ttatatttat ttattagaaa tttagctaca
    17581 ggacatccca atataccacc cataataagt cctattgttg cagcagataa tccaacccca
    17641 actgctcctt ctataccaag attttctatt gtaagtccaa atgcagcaga ataaccatga
    17701 ccaccttcca tagatacatt tccacacata agccctaaaa gaggttcaag ttttataatc
    17761 tttgatattg taatagctaa tatattttga caaaatgcca atactccaca caaaatccaa
    17821 tatctaaata atacactacc acctttcttt ataagattta tgcttatttc aagtcctata
    17881 gttgtaaaaa agaaggttat gaaatttggc attaaatttc cacttataga tatatctgct
    17941 atattaaaat atttaaaaaa taaatttaga aaacaaaata atagacctcc tattactggt
    18001 gcaggtatac agaaattatt taaaaagtta actttattct tcaataaatt accaattaaa
    18061 aagaagaata tagatagtgt caaggtttgc actatatcaa aacttaaggt aatcaaaata
    18121 agtcctcctt tgtaattcaa agttttaatt atataactta aattcttatg tattgaaatt
    18181 ttattatcaa atgtatatta ataaatgtat attaaaaaga atcagtagat ataattagat
    18241 agtcataaat taaattaaaa tattttaatg agatatgact tttttagaat taaaaaccgt
    18301 agtatattga tataatagaa ataagactta aaaatctgtt gatagtatta tttataagta
    18361 cagtaggagg ataaaatgtt tgaaaattta cctaagatag atttacattg ccacttagat
    18421 ggaagtgtta gagtagaaac catgcttgat atagctatta aagagaaaat agatttacct
    18481 tcaaataata tggatgaaat aaaaaaatta gcaaaggttt cttttaattg tacttcacta
    18541 gatgaatatt tagaaaaatt tgatttaccc ttaaaagtaa tgcaatctaa ggaaaattta
    18601 aaaagaatta cttttgaact tttggaagat gcaagtagag aaaatgtaaa atatattgaa
    18661 attagatttg caccattact tcacacccaa aaaggtatga gtgttaaaaa tataattgag
    18721 ggtataattg aaggaattag agaagctgaa agtatttatg atataaaagg caatttaata
    18781 ttaggttgta tgagaacaat gacctcaaaa gaggcattat tagtaattga agaagggaaa
    18841 tcatttgtaa ataaaggtgt tgtagcagta gacttgtgtg gtccagagaa agagggattt
    18901 tgtaaggaat ataaagatgt ttttaaatta gcaagagaat atggatataa agttacaata
    18961 catgcaggag aagctgcaag tggagaaaat gtattggatg ctataaatat tttaaaggcg
    19021 gataggattg gtcatggggt aaaaattaaa gaccataaaa aggcgtataa tttagttaaa
    19081 gataaaaaaa tattgcttga attatgtcca actagtaatg tacaaactaa gacagtagat
    19141 tcttatgaag cacatccatt ttatactttt tataaagata atttacatgt aagtataaat
    19201 actgataata gaactgtatc agatataaat ttaagttcag aactaaatgt tatatttgat
    19261 acttttaaac taggattaga agactataaa ataatttata gaaatgcagt agaagctagt
    19321 tttgcagata aagaaactaa agagtatttg aatagtttaa tttaatagat aaaaatattt
    19381 aaactttaaa ttaagctatt aaattgataa aaatacattg tattaggaga aaaaatgtga
    19441 aagattatga agatgttgtt tatattaaac tagtcaaaaa agaacttgaa gaatggaaga
    19501 aaaatatatt aaagaaacct tctatatttg acaaagcatc aaagggtata cagaataaat
    19561 ttaatggagt attgccagat aaatatcatg aaataattac ttcttctata aaagtaatga
    19621 caaaaaccgt actgtttgga tataaatatg ctacaaagcc atcacttaga aatatgtcat
    19681 tagaagaaag agaaaaatta gttttagaaa aagctaaaac ttacaagact acagcaatga
    19741 ttgaaggggc aggaacaggg gcaggaggaa tcctcttagg tttatctgat tttcctttgc
    19801 ttttaggtat taaagtgaaa tttttatatg atgtagcttc aatatatgga tatgatgtta
    19861 aagattatag agaaaggatt tatatattaa atatattaca gttggctttt tctagtcaac
    19921 agcatataaa caaaactttt aaattgatga ataactggga tgaatatgta aatagtctac
    19981 cagaagacat caatgatttt gattggagaa catttcaaca agagtatagg gattatttag
    20041 atttagctaa gtttttacaa ctaatgcctg gaataggagc atttgtagga ttttatgtga
    20101 atggaaaatt aattgataaa ttatgtgaaa ctgctgtgtc tgcttataga ttgaggattc
    20161 gagagcttaa gttataagag aaattataac ataaaaaatt agttatacaa tatatctata
    20221 tgtatctaat tttgcattga taaaaacaat tatggataaa aggtaaagca tgatagaatc
    20281 taatgtagag gtttgtattt taatggaaaa atttagtttt tattgcttaa gatacaaatt
    20341 gaattacgaa gagaagtaat tgggaggaat attttcatga caaatttgcc aaaacaattt
    20401 gatagcttca atgaagctag acaaaaagga tttataaatg caaaggaact taaggaaagt
    20461 gggaaaaaga tggttggagt tttctgtaca tttactccag ttgaaattcc aatggcagct
    20521 ggagcaactg tagtaggtgt atgcggcgtt agtgaagagc caatacctga tgcagaaagg
    20581 gtgcttccaa gaaatctttg tcctttaatt aaatcaagct atggacatgc aataacagat
    20641 acctgtcctt atttctattt ttctgatttg ttgataggag aaaccacttg tgatggtaag
    20701 aaaaaaatgt atgaagaact tgcaaaagtt aagccaacat atgtaatgca tttgccaaat
    20761 acttcaaaag gagaatttgc ctataaactt tggaaagatg aaatgataag attaaaagaa
    20821 gaggttgaga aatctttagg tgtaactata actgaagaag atataagaac tgctataaaa
    20881 gataaaaatg aagaaagaga acttttaaaa gagttttatg cacttggtaa attacaacca
    20941 tctgcactta caggccttga gttacataat gttttatatc aagcaggttt taaatttgac
    21001 cgtgcagaac ttaaacattc tttaagaaaa gttatagatg atatgaaaga aagatatgaa
    21061 aaaggtgaat gtcctgttca aaaagataag ccaagaatat tgataacagg ttctcctatt
    21121 ggtggaataa gcgaaaaaat agttaaaact ttagaagatg caggggcatc agtcgttgca
    21181 tatgaacttt gtggagctat tagatcaaac gatttattgg ttgatgaaga aatagaagat
    21241 gtatatgatg ctttaacaca aaaatatata aatataggtt gttcttgtat gatgaataat
    21301 gacaatcgta ttgaattatt agatagaata atagatgaat ataatgtaga tgctgtaata
    21361 gatgtagttt tacaagcatg tcatacattt aatatagaaa gttatagaat aagagaattt
    21421 gttacaaaag aaaaaaataa gccatttatg tctcttgaaa ctgattattc aaaatcagat
    21481 actgaacaat taagaactag atttgaagcg tttgtagaga tgttgtagga ataaataaaa
    21541 gtaggaggaa ctatgtttag tataggagtt gactcagggt cagtggcaac aaagggtgtg
    21601 ctatttgatg gtgaaaaaat tattaagaaa ataattatac caactggatg gagccctaaa
    21661 agtacatcta aacaggtcta tgagttgcta agtagtgaaa ttgacaaaaa agatataaaa
    21721 aaggtggtag gtacaggtta tggaagggga gtaatggatt ttgctgataa gaaagtaaca
    21781 gaaataactt gtcatactag aggtatttac tttttaaaca agaacataag gactatttta
    21841 gatgtaggtg gtcaagatag taaagttata aatttagata gagatggaaa tgtatttaac
    21901 tttataatga atgataagtg tgctgcagga actggtcgat ttttagagat tacttctaac
    21961 cttttaggaa gcgatattga aagtatagat acattagcta aaggatatga accagttaat
    22021 atatctagta tgtgtacagt ttttgctgag tctgaaattg taagtcttct agctcaaaac
    22081 atttctactg gagaggtagc agcagggata cttaaatcta ttgcaaataa atccacctca
    22141 atgttagcaa gaggtgaggt aatagatgaa gttgcattta ctggagggct tgcaaaaagt
    22201 aaagaactag taaaaatgat agaagaaata ctgggtaaaa aaatttttat tgcagaagat
    22261 actcaaataa taggagcttt aggagctgct gtaataggat ttagataaaa acatataaaa
    22321 ggaaaatgag aagctgaatt tattataaat aaattcagtt ttttatttta ttttatttat
    22381 ttataaagga aaactttggt ataattgatt taaatgattg tatatttgta aatacttgat
    22441 atttagtcat atataacatt ataattttaa aaattagctc aaaataaggg ggagaaagcg
    22501 tgaaaaaatt tacttcaaaa aaagtaacaa ggtttgttgc gctgttttta atatctattt
    22561 tagtaatgtc aacaattcct gtatcagctg acaatagcaa tacattgaat cagataaaaa
    22621 aagcagaata cagtgagacg tataaacaat atctaaatga tgctaaaaat ggacgtacag
    22681 aaaaatataa tgggattata ccaaatccat atcagttgga aggtactcaa atccaaagta
    22741 aaggaagaac agctccaaca tcttatgacc ctagaaaatt aggtcttatg acatcaataa
    22801 aaaatcaaga ggatttagga atctgttggg attttgctgc aatggcaacc ttagaaagtt
    22861 tcttaaagtt aaataattat ggagactatg atttatcaga agaacactta agatggtggg
    22921 cttcagatgg tgaatatgga tggtctgtaa atgatatgaa tggtgcttta aattatgaag
    22981 ctatgggata tttaacatct tggtcaggtc ctaaactaga aaaagatatt ccatacaatg
    23041 gtagagtatc taaagcacaa ggagcaaaaa aaccaactaa tatgaataca gcacctactg
    23101 tttttaatgt gacagatgct gtgtgtgtaa gtaatgacat aaattctaca aaaaatgcta
    23161 tattacagta tggagcagta acatctggtt attatgaaga tattaaatat caaagtgatg
    23221 accaaaattc atattattgt ccaacaaaaa gttttaatac aaatcatgct atttcaattg
    23281 taggttggga tgataactat tctaaagata attttaatgc aaatattaga ccatcaaaaa
    23341 atggtgcttg gttaataaaa aatagctggg gagattataa ttctgagggt ggatatttct
    23401 ggatatctta tgaagacaag acacttatgt ctgatataga taatttttca atcaaaggtg
    23461 gtaaaaaacc aaatgatgat gagaagatgt atcaacatga ctatgcaagt attgtacctt
    23521 taatttcaaa aaagataaca gctgcaaatg tatttgattt caatagaggc gatgaaactc
    23581 taaaatctgt aatgttctta actgaaagta taggagctaa gtatgaagta tattatgctc
    23641 cagtagtaaa tggtatacct caagaaaaca atatgaaaaa attaaaagaa ggtactgccc
    23701 aatattcagg atacataaca gttccaattg attcttttga tataccagaa ggaaagggtg
    23761 caattgtagt cagtatagag gggaaaaatg gagagtcaac tataggttct gagtcaaatg
    23821 taccaggata tgatacattt aaagcaaaag ctaatttagg tgaaagttat atcatagata
    23881 atactggaaa attctttgat ataaatagag atagtaactt ctatccgtgt aattttacta
    23941 taaaagctgt tacagaaaag tcttctggtg agagtatccc aaatgagtct ttaattggta
    24001 gtgatagata tgaaactgct atcaaagtta gtcaaaatgg tttcaattct tcagaaaatg
    24061 tagttttagt aaatggcagt tcaatagtag atgcattggc agcaactcct tttacatctg
    24121 ctataaattc accaatatta ttgacacaaa aagaagcttt aaattcaaaa actaaagctg
    24181 agatacagag attaggtgct aagaaagtat acctaatagg tggagaaaat tctataagta
    24241 aagaaattga acaacaatta aaaagcttaa atatatcgat agagagaata tcaggaagtg
    24301 atagatacaa gacaagttta ttattagctc aaaagttaaa tggtataaaa aatgtatctc
    24361 aaatagcagt tgtaaatgga gtgaaaggat tagctgatgc aattagtgta ggcgctgcag
    24421 cagcagaaaa taacatacca atcatacttg caaatgaaaa gagcgaattg caaggagctg
    24481 atgaatttct aaattcatta aatatagaga aatcttatat tattggagga acagcatccc
    24541 tatcaaacaa tctagaaagt aagttaaaaa atccaactag attatctggt agtagtagag
    24601 acgaaactaa ttcaaagata atagataatt tttataaaaa agatacttta aaaaatgcat
    24661 ttgtagttaa aaatggaatc aagaatcaaa atgatttaat agatggattg gcagttggtc
    24721 ctttaggagc aaaaactggg tcaccagtaa ttttagttgg tgataaatta gctgatagcc
    24781 aaaaagaagt tcttaaaaat aagactttag aaaaagtaac tcaagttggt ggaggagcaa
    24841 ataaaaatgc atttaaagag ttagtaaaat taaaaagtgc taaatagagt acttaagtat
    24901 taacatatat tagtgtgtat tagtaactta tcttaataaa agctgaaaat aattacttca
    24961 aattattgat aaataagata atcaatgatt gtaaagtttt gttttcagct ttaaatttat
    25021 gtagtataag tttaaaaaca taaaaaaggg ttactttata tagaaatata gaaaatctta
    25081 cattttatat ataaataacc catatttaat taacttttta tagtgttcta atagatatta
    25141 ttgaacttaa tggtaaatat aatgtgtaag tttcatttaa agttggatta gattcatcta
    25201 tatatcttga gcaaagtgta attgataagc tatctgcttc tataatttca ccatatagag
    25261 gacaactgct aaagtaagaa tgtacttcaa catatttatt taagttttct tgaacataat
    25321 cgtaaaaacg gttttttgta gtagtagcca taaaatcaac tcctttattt tttattaaag
    25381 ttgaaacttt taaaaagata aatatgatta cttctattag aaactaagtt aaaaaataag
    25441 caaa
//
LOCUS       DAYAPA010000050        25169 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.050, whole genome shotgun sequence.
ACCESSION   DAYAPA010000050 DAYAPA010000000
VERSION     DAYAPA010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25169
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.050"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(155..859)
                     /locus_tag="ACGI59_001903"
     CDS             complement(155..859)
                     /locus_tag="ACGI59_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B3/4 domain-containing protein"
                     /protein_id="HFG9739689.1"
                     /translation="MKKFVIEDAFWNLFPSAKIGVVICNDIDNSIKDEDYKKIILKGE
                     EEALKYLEDSEFSNNQVIKVWREAFKKFKTKKGARSSIEALLKRVHNGNNIGTINPLV
                     DIYNFISLKYALPCGGEDIDKFIGDIRLTRAIGNEEFIPLGTDENSFPYEGEIIYKDD
                     GGAICRCWNWREAVRTMLTEDTNNAFLCIELVDESRFKEFENALNELAKIVQEKLGGN
                     CKISILDIDNKEVSVD"
     regulatory      complement(923..1017)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00442"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="guanidine-I (ykkC/yxkD leader) riboswitch;
                     Guanidine-I riboswitches control genes whose products are
                     involved in modification or pumping out guanidine as a
                     toxic compound from bacteria; This is class of guanidine
                     riboswitches is based on the ykkC/yxkD leader; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="guanidine"
                     /db_xref="RFAM:RF00442"
     gene            complement(1213..2229)
                     /locus_tag="ACGI59_001904"
     CDS             complement(1213..2229)
                     /locus_tag="ACGI59_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HFG9739690.1"
                     /translation="MGYYNDNDNNKIRRKKISNSKDSNKKSKHSYTPANKKNKSSKTS
                     SSKRMSEKVDSQNLIKRDRDSFTRDNVRPLNTDYNQRERRRVEYKQREKKLKSKKRKK
                     QKRIRIVRALLSIMLVALIIFSVAGAIFTVSAIKGSPEVTKKLIRSKYISSEVVSIKQ
                     MPNDLKHAIVSIEDERFYKHKGVDVISLVRSVLNNVFTDTTQGASTIDMQVSKNLLTS
                     NDKTMKRKIRDMYNAKQMNKIMTKDEILEAYLNNMYLGKNAYGAAKGAEVYFGKKVSE
                     LNLAECAMLAGMTNNPARYIDHGEAKKRQVIVLYKMHQLGYITDKEYRIAKADDTPFK
                     SEID"
     gene            complement(2357..2863)
                     /locus_tag="ACGI59_001905"
     CDS             complement(2357..2863)
                     /locus_tag="ACGI59_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HFG9739691.1"
                     /translation="MKEYVVVDLETTGLDPYKGCEIIEIGITEIKNEQIVKNYSRLIK
                     PKGIISSFITELTHISNEMVENEEPLELVLPRFRKYIGDRTIIAHNAKFDLKFLNYYL
                     RMLDLEPINNYICTVELLKKCKSYKGKNKKLETACAYYNIENINAHRADSDTLATAKL
                     FLKIKDEY"
     gene            complement(3022..3690)
                     /locus_tag="ACGI59_001906"
     CDS             complement(3022..3690)
                     /locus_tag="ACGI59_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MORN repeat-containing protein"
                     /protein_id="HFG9739692.1"
                     /translation="MENITSYFTTILCVFICLSSVFIFTQLARVFINKKKINQKIKSR
                     NGFRYDRDFIEARREEIHIKDNNNNKNKSNNKKLKGEKVFKYDNGDLYKGEFVDGKKN
                     GFGIYIFSSKEKYEGLWKDDKMHGIGKYTYRDGSIYTGEFKYGLKNGLGKLTYPNNDI
                     YKGYFLDNKKNGKGVLYKNDGNKQAGIWEDDEQCKSLDFKDLNNNKINNVYQNRLNNN
                     KLKV"
     gene            complement(4103..4609)
                     /locus_tag="ACGI59_001907"
     CDS             complement(4103..4609)
                     /locus_tag="ACGI59_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HFG9739693.1"
                     /translation="MGFREVKIEELQFNPFTKIGKEWLLITAGDSEKFNTMTASWGGV
                     GVYWGKNVVTTYIRPQRYTKEFVDSNDTFTVAFFDETYREALNICGTISGRDINKIEK
                     AGLTPYFVDDTVAFEEANMIIVCKKLYHGNMPPENFDAKENDKKWYPEKDYHTMYISE
                     IIKVLVKE"
     gene            complement(4980..6008)
                     /locus_tag="ACGI59_001908"
     CDS             complement(4980..6008)
                     /locus_tag="ACGI59_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HFG9739694.1"
                     /translation="MKRVYIDFEMNMPNSKSKRAVFNSDIIAIGAVVYDEKTKNIDKF
                     KSLIRPVSNEELYPHIQELTHISSEELKSAPSYEEVMRKFKKWLGIFSDIKGIYTFGN
                     LDLTCFNNTDMRSAKKNNHPRFVNNIRDLFVDIKEKYINCGMRCMNYISLKNLLEFVN
                     LEFSGDAHDPLNDAYNLFILDEAITNNVDIQNLLIIRDIIRPPFNDINSNLDNCFNKF
                     KESLYKKEGNYNIVDFSVEIIKTVRMYLLTIIDVNIQNLEIMKDISKKMDTIDKLKDI
                     EEGYFYLLEDVYFDMKDVLEDLMLYRMNEDEYKYEIKNIIKMFDEDLDNEKIYINKCN
                     NLNVVNKI"
     gene            complement(6056..6916)
                     /locus_tag="ACGI59_001909"
     CDS             complement(6056..6916)
                     /locus_tag="ACGI59_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HFG9739695.1"
                     /translation="MSELSGKGCEIIVPFEERLPVRDIEKSIIKKYRKNLWSKFMKAI
                     RDYKLVEEGDKIAVAISGGKDSILMAKMFQELKKHGQVNFDVEFIAMDPGYHANIRQL
                     LIDNCEYLNIPIHLFDSRIFEIADEIAKDYPCYMCARMRRGALYSKAEELGCNKLALG
                     HHYDDVIETTMLNLLCAGNFKTMLPKLNSTNFEGIKIIRPLYYIREEHIIRFIQNSGI
                     WPLNCACMVAAKKTGNKRYEIKDLIKSLESNFKNVEKSIFKAAENVNLDSVLGWQKDG
                     EKHSFLENFE"
     gene            7185..7394
                     /locus_tag="ACGI59_001910"
     CDS             7185..7394
                     /locus_tag="ACGI59_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat associated protein CotJA"
                     /protein_id="HFG9739696.1"
                     /translation="MENKTRDIKKNCGPSLARPYINNQIFTEMYCLSDALKFGTIFPE
                     LNLLESEMYNKELYAKPKKYRGGRR"
     gene            7394..7660
                     /locus_tag="ACGI59_001911"
     CDS             7394..7660
                     /locus_tag="ACGI59_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein CotJB"
                     /protein_id="HFG9739697.1"
                     /translation="MKDSSRAELMRKVQETSFACVDMNLYLDNHPDDKNAINTYNSLC
                     NQFAQARYAYENKYGPLTNFGYAPSRCPWQWADQPWPWDREFNY"
     gene            7689..8261
                     /locus_tag="ACGI59_001912"
     CDS             7689..8261
                     /locus_tag="ACGI59_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HFG9739698.1"
                     /translation="MWIYQKTIQHPVNIKTCDPRMAKFLITQFGGPNGELAASLRYLS
                     QRYTMPTGNMRALLTDIGTEELAHVELICTMVYQLTSDASPEELKAAGLGSNYAQNGY
                     GIYPTDSNGVPFDVRPIAVMSNPVTDLHEDMAAEQKALATYYQLINLTDDVDVIDVLK
                     FLGQREIIHYQRFGEALMDAYELEESQKMF"
     gene            complement(8496..9686)
                     /locus_tag="ACGI59_001913"
     CDS             complement(8496..9686)
                     /locus_tag="ACGI59_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HFG9739699.1"
                     /translation="MNFNQKRIAASIMATAIIMPTMGNLAYANESEVESVSIESRTIT
                     GNAVNFRKGPGTNHESMGKLYKGDKVEYVGKEGSWVKVKYNGNTGYVHGNYVAINSLG
                     SSNESSDTSVKSTKVVTAKGLNFRTGPSTSSSKISTLGYGTEVGYISESNGWSKISSN
                     GRVGYVSSKYLGTSVNDSTNENAENSSNDLVKGTKVVTAKSLNVRTGPGTSHSKIATL
                     SYGTEVGSISESGGWTKVSYGNQTGYVSSQYLAEKGSVDTSIPSYSTNSPSQGADSVI
                     SFAKTLLGKPYVWGAEGPNSFDCSGFTQYVMKKSVGVSIPRVSRDQSKYGTYVNRGDL
                     RSGDLVFFDTQGSNNGSVSHVGIYIGNGDMIHASSGSSKKVTISNINSSYYSSRYVNA
                     RRVL"
     gene            complement(9946..10377)
                     /gene="dut"
                     /locus_tag="ACGI59_001914"
     CDS             complement(9946..10377)
                     /gene="dut"
                     /locus_tag="ACGI59_001914"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFG9739700.1"
                     /translation="MYNLKVKKLNDDAIIPNFAHKGDAGMDLYSIEEVVIPPGETKLI
                     KTGICIELPTMTEAQVRPRSGLALKHSVTVLNTPGTIDEGYRGELKIILINHGKNDFK
                     VEKHMKIAQMIVKPIYDINIEEVKELSDSERGKGGFGSTGF"
     gene            10490..12718
                     /locus_tag="ACGI59_001915"
     CDS             10490..12718
                     /locus_tag="ACGI59_001915"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HFG9739701.1"
                     /translation="MKLVLAEKPSVAKTIASFLGAKTRQDGYFEGNDYIITYAVGHLV
                     SLYDMKDYDKDKYSGSWKMNNFPFVPEDKFKFKIDSSKTKQFNTVKKLLNRKDVEYVI
                     NATDNDREGELIAFLIFLLAKNKKPVKRILVNEWTPEDITRGIKNLKDEDEMRNLQAA
                     GYTRLITDWLIGINFTSVATLKYGNGKLLNIGRVILPTVKLVYDRDMEILNFVPKTYY
                     EIEGHFKAEAGEYKGKYVKGKESKFDTLEDANKIIASITSETGKILDKKVTMSKEYAP
                     KLFSLTSLQGYITSKYSNFTSDKVLNVCQSLYEGNGKGGYITYPRTDSIFLEESLASK
                     ASQTLDKLKVGLEYENKIKFSKTKRVFDSSKVDSHSAIIPTYIIPKSLSKDEQLVYDA
                     IKDRFVANFMPPAEYENTEIKTEVDNCTFLTKGKVLKSKGYLEVYNKEEKNDLLPLVN
                     KDDVVDVLEIKPLTKQTTPPKPYTEDTLLKAMKNCGKNVPEEDTTVLSGYSIGTSATR
                     ADVLKKISQVGYVKKKGKSYSITDLGKNLVEIFPVKDLFDVDYTGKLEKSLSDIQKGK
                     YTRKEYLTNIISFIYQNVNIIKQDASKNINTEAYTFDSKTKKFTKTKEKKQKKDVTNT
                     EKEVAISKDSNNKKEVKNGDENQSLGKCPICQGDVLEFDKGFACKNHKECKFVIWKND
                     KYLSLYKKKVNKTMVKNILKKGETKVKSLTAKNGNKFDATLKYDKNPDTGYFNWKIEF
                     DN"
     gene            complement(12829..13314)
                     /locus_tag="ACGI59_001916"
     CDS             complement(12829..13314)
                     /locus_tag="ACGI59_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFG9739702.1"
                     /translation="MDNNNILKIADSFLDFLFVLENNIFKESDLLKKFQNNSDIMKAY
                     FEECPMAPSHAKVILYLMTSNSSSISQIASNLGILKSNMTPIIDRLVEHGLVNKFPDP
                     KDRRILRVELTDKAFKLFDAVKAILKESLVKKLSNLSEEELTLLDEHTLKLSEIVKKL
                     G"
     gene            13566..14789
                     /locus_tag="ACGI59_001917"
     CDS             13566..14789
                     /locus_tag="ACGI59_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFG9739703.1"
                     /translation="MKRYLIVFLMCSSLFLVGCGKTEESEPEKPIAVSVQKAIGGEIE
                     NTNSFSGTTKVKDETAVTAQTVGTVQEVYVKLGQNVRKGDELLSISSPELENSVKQSK
                     ASLDLAKASYSSATGGSLEAQVNQAKTALDNAKIQYEESQRNYDNNKILYEQEVISLD
                     QFKKIEFSLEQTKQQLDSAQRAYDTATSKSIPQAKALAKKQLDQAQVSYNLAMSNLDK
                     LTLTSPVDGTITAKNFDSKEMITQSQPAFIISNPSILEVDLNVAESDIGKFKKDGNVD
                     VIIEDQRILGKIDYVPSVVDPQTSLYPVKVLVNNTNNKFKAGMSAQVNLSVEKENGAV
                     TVPKKAIFEENGKKYVYIATKDNIAKKHLVQTGIVTEDKIEIKSGVSDKDTVIIGGIS
                     LISDGTKIFPVEKEK"
     gene            14793..17927
                     /locus_tag="ACGI59_001918"
     CDS             14793..17927
                     /locus_tag="ACGI59_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HFG9739704.1"
                     /translation="MNLTQTSVKRPLTIIMVFLVLIVFGGIGYKKMSINLMPDIEIPV
                     VMVMTTWTGAGPQDVDEQVSQKVDESLSAVSNVKSTISSSQESVSMVVAQFEFGTNLD
                     EIMNDVRSKVDALQTSLPDDAAKPTVLKLDMNAQAIGQLVISGGNENSSQALRKYAED
                     VIQPKIESIDGVTSADLKGGKKAQVNVIADPAVLSNYGVSLSTIKGVLSSSNKTFPYG
                     SITQGEDKIVLRAIDKLESLDDIKQIQIPVKGGNTVRLDNLCSVDYGYADKDSIYRYN
                     GKDSLVIDISKQQDANTVKVMQSAYKYVDELNKENPQFKIKIANDTSSYISESINSVM
                     SNLLISAVIAFIVIFAFLKSVRASLVVAVAIPISIIGAIAILYFTGESLNLITASSLV
                     ISVGMVVDNATVVIENIFKYRKSGRLSLDDCAIEGTRTVTNAILASTLTTIAIFLPIM
                     FTEGIAKITFGALGKTLIASLAFSFIGAITLVPSVFAKLSRGKNSQKMQEKDSPIFDK
                     VSEAYKKLLSVCLRHQRLVVISSLLIFVASLFGMGFIGMDFMPAADKGELSISIELPK
                     GLSLESNDYYVSMTEKKVSDIPEIKTLITTLTSDSNNSKASIAIELESQKNRKRTTDE
                     IEKEIIDRVATVPDCKINVSQNSSVMGGAGGADFTLEMKGPDLDVLKILGKEAEKEFS
                     SIKGFRNVETSLTDTTQEAQFTIDKHKAQEYGVNTAEIAGMLRTAINGDSVTTATIDD
                     YKVDVNLKFKADTIDSLEDIKQLKVNSATGKEVPLGAFSDIKMADGLQSISRTDGDFS
                     VNITATLDNLDAGTATKLALEKTNKMVIPKDYSIGVGGETEMMNESMASLGFSMVIAI
                     ALVYMVMVAQFESFSKPFIIMFCVPFAFVGVVLSLLVSGQSLSTVGMLGIILLIGIVV
                     NNGIVLIDYIEQLRKTNRNNDLIGLVSIGCAARLRPVLMTTATTILGMLPTALSLGEG
                     GETMQPLAIVIMGGLTVSTLITLVLIPTIYLIFDKWENKFSDKMPKIFGKITDKIGSI
                     KRKIIKKPKLKAKV"
     gene            17949..19259
                     /locus_tag="ACGI59_001919"
     CDS             17949..19259
                     /locus_tag="ACGI59_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HFG9739705.1"
                     /translation="MNKKMSKIVAIGCGVGIITANLIPVYADNNTSSINKVDNSILSD
                     SNKRTSEKEILTAREAVSAALKNSEKLKMKSEEIKMLKEKLEVQDEFDSFTGSDNSFP
                     YDQIGLLKNQSEQAKGFMEDQIANDITNKFNDLVSRENELDKIKNNLEIKTKEIKDMK
                     LKKDLGLVTSLETESAELELQTLQNTQKAKLQELKNNQDYFKLLTNIDLNNYQLDKEY
                     RFESFRVGGSVDSYMEGKVNEYLKYDQLILERTEESFNDKDENKADLPDRPNLTRPVA
                     PTKPEQGNLTDDEYKVLTDKYEKDYKRYEKEVEAYNLERQTYAAGLTTYANYLQQKFN
                     TENGLVTLEDSKKALKKGLIDSYAQLLALEDTIQITKKQLDLSEKQLKNTKLRYDLGL
                     ITLTDYKKQVVSNEDAKNSYDTLIVNYNSLKNGIEKPWILNTGK"
     gene            complement(19548..19793)
                     /locus_tag="ACGI59_001920"
     CDS             complement(19548..19793)
                     /locus_tag="ACGI59_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFG9739706.1"
                     /translation="MTKCPKCGKEVSSRPGSICPRCGFKVADESLKIRCPEPSCRALV
                     SRKLDYCPKCGCKLRGYNISEFVNMVRCKIDETFDKK"
     gene            complement(20007..22634)
                     /gene="ppdK"
                     /locus_tag="ACGI59_001921"
     CDS             complement(20007..22634)
                     /gene="ppdK"
                     /locus_tag="ACGI59_001921"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HFG9739707.1"
                     /translation="METKYVYSFGEGSKDMKSLLGGKGANLAEMTKIGLPVPPGFTIT
                     TEACNDYYVNNESIRAEIIKEIEEHLATLEKDLNKTLGCNKNPLLVSVRSGAVFSMPG
                     MMDTILNLGLNDNSVVGLAEATQNERFAYDSYRRFIQMFSDVAMEVPKYKFENVLDRV
                     KEAKGYTVDTELTTDDLKEIVKEFKAIYKKEIKNDFPQDPKEQLMLAIEAVFRSWNNP
                     RAIVYRKLNDIAHNLGTAVNIQSMVFGNMGETSGTGVAFTRNPATGENKLFGEFLMNA
                     QGEDVVAGIRTPQNISTLADVMPAVFDEFVKITHILEGHYKDMQDIEFTIENERLYIL
                     QTRNGKRTAAAAINVAVDLVEAGIIDEKEAIMRIEPNQLDQLLHPKFEDKALKEAKVI
                     AKGLPASPGAASGKVYFNADDVVKANEKGEKVVLVRLETSPEDIEGMVKAEGILTARG
                     GMTSHAAVVARGMGKCCVAGCGEIKVDEFNKEIRALDDVVIKEGEYISIDGSTGNVYL
                     GDVKKTEVSLTGNFEKLMNWVDKHKCMMVRTNADNPRDARAAIEFGAEGIGLCRTEHM
                     FFDEARLPAVREMILSNTVEQREKALEKILPMQREDFVELFKVMDGKPVNIRLLDPPL
                     HEFLPHDDETIEELSKSMGIKVSDIKKRIVDLDEFNPMLGHRGCRLAITYPEICVMQS
                     KAIIQGAIEAIKAGVKVSPEIMVPLVGEVNELKIIRKMIVETVDAIIKEEGIEVPYTV
                     GTMIEIPRACLTADEIAQEADFFSFGTNDLTQMAFGYSRDDAGKFLGQYVDEEILEKD
                     PFQVLDQNGVGKLVKMGAKLGREVKPELKLGICGEHGGEPSSVEFCYSVGLNYVSCSP
                     FRVPIARLAAAQASIKNPR"
     gene            complement(22695..23537)
                     /locus_tag="ACGI59_001922"
     CDS             complement(22695..23537)
                     /locus_tag="ACGI59_001922"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HFG9739708.1"
                     /translation="MLVKNLIIYAVSDSVGETAQQVAKACMSQFYVNETYEIKRFPYM
                     INKGVLLETLENAKAENALIVYTLVDEELCSIVERYCEREGLSCIDLMTDILREISKR
                     TGRKPKREAGIIRKLDESYFKRVEAIEFAVKYDDGKDPRGVLQADIILVGISRTSKTP
                     LSMYLANKNIKVANVPLVPEIPIPKEVFEIDTKKIIGLTNSPEKLNEIRTQRLKALGL
                     SSKANYANLERILQELDYSEEIMKRIGCPVINVSNKAIEETAGIILDIMKENGLKIYK
                     EIEI"
     gene            complement(23544..24182)
                     /locus_tag="ACGI59_001923"
     CDS             complement(23544..24182)
                     /locus_tag="ACGI59_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFG9739709.1"
                     /translation="MIIIQLNERQLKIIDIVKENEPITSESIASSLNVTRATLRSDLA
                     ILTMTGILDARPKVGYFYSGVSEINLIGKSVKEKTVEDIMSMPVLAKKDESIYDVIVT
                     MFLSDVGSIVIIDENEELCGVVSRKDLLKATIGGSDINKMPIGMIMTRTPNVVTLTKG
                     ASVLLASRKIIEHEVDSIPIVEYKGEDKNHMRVVGRISKTNITKLFLEIVDN"
     gene            24455..25066
                     /locus_tag="ACGI59_001924"
     CDS             24455..25066
                     /locus_tag="ACGI59_001924"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HFG9739710.1"
                     /translation="MINDIKNKFKNYKPYINGYKNMKRASVLIPIVEINNTHYILFEV
                     RSKNLKHQPSEISFPGGKIESGETPYEAVIRETCEELGTFSDNIEVISSLDLLITPVK
                     FIIHPYLGYIKNINNLNINKDEVDHTFLVPIKYLLENPPNTYSNTVKVFPDENLPYGS
                     IPNQKDYKFESGSYPILFYKYKDYVIWGMTAKILENFLNFLSE"
BASE COUNT         8698 a         4010 c         3201 g         9260 t
ORIGIN      
        1 tacatttttt tcttatttta aaggcttttt atattctaca tttttatttt ctatattata
       61 tcctttgata atcgttatat ctaaaataga ccttcttacc ctaaatataa gtatctttat
      121 aactagaata gtccattaca acagcacctc catattaatc cactgatacc tctttattat
      181 caatatctaa aattgaaatt ttacaattcc cacccaattt ttcttgaact attttagcta
      241 attcatttaa agcattttca aattctttaa atctactttc atctactaat tcaatgcata
      301 aaaaggcatt gttagtatct tcagtaagca ttgttctcac tgcttcacgc caattccaac
      361 atctacaaat tgctccacca tcatctttat aaattatttc tccttcatat ggaaatgaat
      421 tttcatctgt ccctagtggg ataaactcct cattaccaat tgctctagtt agcctaatat
      481 ctcctataaa tttatctata tcctcccctc cacaaggtaa tgcatatttt aatgagataa
      541 aattgtagat atccactaaa gggttaatag ttcctatatt atttccatta tgaactcttt
      601 ttaataatgc ttcaatagaa gaccttgctc cttttttcgt tttaaatttt ttaaatgctt
      661 ctctccatac tttaatcact tggttgttgc taaattctga gtcctccaag tattttaatg
      721 cttcttcttc tcccttcaaa attattttct tatagtcttc atcttttata gagttatcta
      781 tatcattaca aataacaact ccaatttttg cactaggaaa tagattccaa aatgcatcct
      841 caataacaaa ttttttcatt agaaaacctc ctcaaattta aaatatgtaa aaataaaaaa
      901 gcctatagaa atattcttaa atatctccca ggcttttatc cttccgtgta cacaacaatt
      961 gcgcgttttc tcttggacct gaccattata aaatgcggaa ccctagaaaa ctaaattata
     1021 agttttttaa tttacaaaat acaattaaat tttagcataa tatagcattt aagacaatat
     1081 tctattatct agtttgtaca ttaaaacaat ttgccattag attataatta tggtggcaaa
     1141 tgggtggcaa atcacctttt ttattacatt tttgtttgtt taaactattg aaaatactat
     1201 attcttacaa ttttaatcaa tttctgattt aaatggagtg tcatcagctt ttgctattct
     1261 atactcctta tcagttatat aaccaagctg atgcatctta tacagtacaa tcacttgtct
     1321 cttcttagcc tctccatggt caatatacct tgcaggatta tttgtcatac cagctaacat
     1381 agcacactca gctaaattta attcactaac ttttttacca aagtagactt cagctccctt
     1441 tgcagctcca tatgcatttt tacctagata catattattt aaatatgctt caagaatttc
     1501 atccttagtc ataatcttat tcatctgctt agcattatac atatccctta ttttccgctt
     1561 catagtttta tcattactag taagcaggtt ttttgaaact tgcatatcta tagtacttgc
     1621 accttgtgtt gtatctgtaa acacattgtt taatactgac ctaactagag aaataacatc
     1681 tactccttta tgcttataaa atctctcatc ttctatggaa actattgcat gttttaaatc
     1741 atttggcatt tgttttatac ttacaacctc actacttata tatttagacc taattaactt
     1801 tttagtaact tctggtgacc cttttatagc tgaaactgta aatattgcac ctgccactga
     1861 aaatataatt agtgcaacta acataataga taataatgct ctaactattc taatcctttt
     1921 ttgttttttt ctttttttac tttttaattt tttctctctt tgtttatact cgactcttct
     1981 tctttctctt tgattgtaat ctgtatttaa aggtcttaca ttatctcttg taaaactatc
     2041 tctatctctt tttataagat tctgtgaatc tactttttca ctcattcttt tactagaact
     2101 tgtcttagaa cttttatttt ttttatttgc cggagtataa gaatgtttag attttttatt
     2161 actatctttg ctgttactaa tttttttacg tcttatttta ttattatcat tatcattgta
     2221 ataacccatt ttagtctccc ttcttcatat aaggtacatt atacattgtt ctacaaaata
     2281 aatcaaagaa caaaattgta agcttaaggg tcaattttat aaaggatatt ttcactaatt
     2341 ttctctatat ttataattaa tattcatctt ttattttcaa aaatagtttt gcagttgcta
     2401 atgtatcact gtcagctcta tgagcattta tattctctat gttgtaataa gcacaagctg
     2461 tttcaagttt cttgtttttt cctttataac ttttacattt ttttaataac tctactgtac
     2521 atatataatt gtttattggt tctaaatcta acattctaag atagtaattt aaaaatttta
     2581 aatcaaattt cgcattgtgt gcaattatag ttctatcacc tatgtatttt ctaaatctag
     2641 gtaaaactaa ttctagtggt tcttcatttt ctaccatttc attacttata tgtgtcaatt
     2701 ctgttataaa tgaagaaatt atccccttag gttttatcaa ccttgaatag ttttttacaa
     2761 tttgttcatt ttttatttct gttattccta tttctattat ttcacaacct ttatatggat
     2821 ctaatcctgt agtttctaaa tccacaacta catactcttt catatctctt ctccttatta
     2881 ttaaatggat atatttatat tctatattta tgtaatagac taatcaatac ttaattttat
     2941 atgatatagg aagattttac aaattacaac atagatatat cctatattcc atcataaata
     3001 aagatttttt tattagaata attaaacttt taatttattg ttatttaatc tattttgata
     3061 aacattattt attttattat tatttaaatc tttaaaatct aaacttttac attgttcatc
     3121 atcttcccat attcctgctt gtttatttcc atcattttta tataatacac ccttaccatt
     3181 tttcttatta tctaaaaaat atcccttata aatgtcatta ttgggatatg ttaattttcc
     3241 caaaccattt tttaatccgt atttaaattc acctgtatat attgaaccat ctctataagt
     3301 atattttcct attccatgca tcttgtcatc tttccataat ccttcatact tttccttact
     3361 tgaaaaaata tatatcccaa atccattttt ctttccatca acaaactcac ctttatataa
     3421 atcaccatta tcgtatttaa atactttttc tccctttaat ttcttattgt tactcttatt
     3481 tttattatta ttgttatcct ttatatgtat ttcttctctt ctggcttcaa taaaatctct
     3541 atcataccta aatccgtttc ttgatttaat tttttgatta atcttcttct tattaataaa
     3601 tactctagct agttgagtaa atataaatac agatgataag catataaata cacatagtat
     3661 tgttgtaaaa taactagtta tattttccat aaaaaatcac cctttaaaaa atttatttaa
     3721 aatacctaat ataatatata tttttattaa atgttattta tacttttata gtactatatt
     3781 gaataaaatt aactttgaaa ttatttacat taaaataaat caaatacaaa tgccataaat
     3841 taagtgtttc tagttagcat aaaaatactt ataaagtata ctcaaatatt tcaaataaat
     3901 acttttattc aaataaatct ctacttatat aagtgcttat ataaataaaa agatgaacta
     3961 tagtaaatat agttcatctt ttttataata gtatttattt aaaattaata tttatttagt
     4021 tactagcatc tacttagata tcagataaga aagcacagtc atttacttat aataagttct
     4081 tttcatatat tttttattat aattattctt ttacaagaac ttttataatt tctgaaatgt
     4141 acatagtatg gtagtctttc tctggatacc attttttatc attttcttta gcatcaaagt
     4201 tttctggtgg catattacca tgatatagct ttttacatac tataatcata ttagcttctt
     4261 caaaagctac tgtatcatcc acaaaatatg gtgtaagtcc tgctttttct attttattta
     4321 tatctctacc agaaatagta ccacaaatat ttaaagcctc tctatacgtt tcatcaaaga
     4381 aggcaactgt aaatgtatca ttactatcta caaattcttt tgtatatctc tgtggtctta
     4441 tgtaagttgt aacaacattt ttaccccaat agactccaac accaccccag ctagcagtca
     4501 ttgtgttaaa cttttctgaa tcaccagctg ttataagtaa ccattctttg ccaatcttag
     4561 taaatggatt aaactgtaat tcctcaattt ttacttctct aaaacccatg acttcatcct
     4621 acccttcatt ttttaattat ataaattaat tgttaaaaat ttgaatatat gtactcttat
     4681 aatactgact tttgattgtc ctacataatt catgtaaatt ataatacaac aaatcaatta
     4741 ctttcgcaat aagtagtttt catattttat aagtgtaaaa tatagataaa atatattgat
     4801 tataaagtat ataatatatt tttaaaccta aagcaatata caataaaaaa atgtagaaag
     4861 taagtatctc tctacatttt ttattgtata ttatttttat attaatttaa ttttatcttg
     4921 attcagtttt ataaaaaatt gttataattg tcaagttcta ctctattatt tataatttct
     4981 tatatcttat taacaacatt taggttatta catttattta tatatatttt ttcattatct
     5041 aagtcttcat caaacatttt tattatattt tttatctcat acttgtactc atcttcattc
     5101 attctataaa gcattaaatc ttctagtaca tctttcatgt cgaagtacac atcttctaat
     5161 aaataaaaat agccttcctc tatatctttc aatttatcta tagtatccat ttttttactt
     5221 atatctttca taatttctaa attttgtata tttacatcta tgattgttaa taagtacatt
     5281 ctcacagttt ttattatttc aacagaaaaa tctacaatat tataatttcc ttctttttta
     5341 tataatgact ccttaaattt gttaaaacaa ttatctaaat tagaatttat atcgttaaat
     5401 ggtggtctta ttatgtcacg tattattaaa agattttgta tatctacatt atttgtgatt
     5461 gcctcatcca aaataaataa attatatgca tcatttaatg ggtcatgggc atctccacta
     5521 aactctaagt taacaaattc aagtaaattt ttcaatgata tatagttcat acatctcata
     5581 ccacaattta tatatttttc ttttatatct acaaataaat ctctaatgtt atttacaaac
     5641 cttggatggt tatttttttt agcacttctc atatctgtgt tattgaaaca tgtcaaatcc
     5701 aaattaccaa aagtatatat tcctttaata tcagaaaata tgccaagcca ttttttaaac
     5761 tttctcatga cctcttcata acttggtgca ctcttcaatt cttcagaaga tatatgtgtt
     5821 aattcttgta tatgaggata caactcctca ttacttactg gtcttattaa agatttaaat
     5881 ttatcaatat tttttgtttt ttcatcatac acaactgctc caattgcaat tatatcagaa
     5941 ttaaatactg ctctttttga tttagaattt ggcatattca tttcaaaatc tatatatact
     6001 cttttcaata taaacaccct ttccaattgt ttatagtgat tctttttaac tatttttatt
     6061 caaaattttc taagaaagaa tgtttctccc catctttttg ccaaccaagt actgaatcaa
     6121 gatttacatt ttctgccgcc ttaaatatag atttttctac atttttaaaa tttgattcta
     6181 aactctttat taagtctttt atctcatatc ttttattacc tgttttttta gcagcaacca
     6241 tacatgcaca atttaatggc catataccac tattttgaat aaatcttata atatgttctt
     6301 ctcttatata ataaagtggt cttataattt ttataccttc aaaattagta gaattaagtt
     6361 taggaagcat tgttttaaaa tttccagcac acaataagtt aagcattgtt gtctctatta
     6421 catcgtcata atgatgacct aatgcaagtt tgttgcaccc tagttcttct gcctttgaat
     6481 ataatgctcc tctcctcatt cttgcacaca tatagcatgg ataatctttt gctatttcat
     6541 ctgctatctc aaatatcctt gaatcaaata aatgtattgg tatatttaga tactcacaat
     6601 tatctataag taattgtctt atatttgcat gatatccagg gtccatagca ataaattcaa
     6661 catcaaaatt aacttgtcca tgttttttta actcttgaaa cattttagcc ataagaatac
     6721 tgtcttttcc accagaaatc gctacagcta tcttatcacc ttcttcaacc aatttataat
     6781 ctcttattgc cttcataaac ttagaccata aattttttct atattttttt attatacttt
     6841 tttctatatc tcttactgga agtctttcct caaaaggaac tataatttca cagccttttc
     6901 ctgatagttc actcatctaa tcacctcgta tatttatgtt aataatattt tacaaaaaag
     6961 tctaccttca tccacaaaat actataattt ttactttaat tttactaaat aatttatagt
     7021 ttacaataac acataaactt ataataacat aaataacctc tttcttttat tatctttttt
     7081 atttttctca tttaatattt tattattagt aacgattttt aaatgattga atattataca
     7141 ctagatagag ttacctatct atttacaaca aggaggtttt catcttggaa aataagacta
     7201 gagatatcaa aaaaaattgt ggtccatctt tggctagacc atacattaat aatcaaattt
     7261 tcacagaaat gtactgcctt tctgatgcat taaaatttgg aactatattt ccagaactaa
     7321 acttactaga gtctgaaatg tacaacaaag aattatatgc taagccaaaa aaatatagag
     7381 gaggtagaag ataatgaaag actcatctag agctgaactt atgagaaaag ttcaggaaac
     7441 atcttttgca tgtgttgata tgaacttata tttagacaat caccctgatg ataaaaatgc
     7501 tatcaacact tataattcat tatgtaatca atttgcgcaa gctagatatg cttacgaaaa
     7561 taaatacggt cctttaacta actttgggta tgctccaagt agatgccctt ggcaatgggc
     7621 tgaccaacct tggccttggg acagagaatt taattattag acatcaagta ttggaggatt
     7681 gaaaattcat gtggatatat cagaaaacta tacaacaccc agttaatata aaaacttgtg
     7741 accctagaat ggctaaattt cttataactc aatttggtgg gccaaatgga gaacttgctg
     7801 catctttaag atatttaagc caaagatata caatgcctac tggtaatatg cgtgcacttt
     7861 taacagatat tggtacagaa gaactagctc acgttgagct tatatgtact atggtttatc
     7921 agttaacttc tgatgcaagc ccagaagagt taaaagctgc aggtcttggt tcaaactatg
     7981 ctcaaaatgg atatggaatt tatccaacag attcaaatgg tgttccattt gatgtaagac
     8041 ctatagcagt tatgtcaaat cccgtaaccg atttacatga ggatatggca gctgaacaaa
     8101 aagcacttgc aacttattat caacttataa acctaacaga tgacgttgat gttatagatg
     8161 tattaaaatt cttgggtcaa agagaaataa ttcactatca aagatttggt gaagctttaa
     8221 tggatgctta cgagttagaa gaatctcaaa aaatgttcta aaatttttat ttgtataact
     8281 attattattt gataatttat aatttactgt tgtgttaaaa tttataaaaa atataaatat
     8341 aatttatttt ctataaacag agaaaataaa taaaaagcag gtatcaatca aatagtaaca
     8401 tgtattatac tatacattac tatcaatatt gtacctgctt tcatattata agcaataaag
     8461 aaaaatctat ttattaggtt ttataataaa aacaattata aaactcttct tgcatttaca
     8521 tatctacttg aatagtagct actatttata ttagatattg ttactttttt tgatgaacct
     8581 gaagatgcat gtatcatatc tccatttcct atgtatattc caacatgact tacagaacca
     8641 ttatttgaac cttgtgtatc aaagaatact aaatctccac ttctaaggtc tcctctattt
     8701 acatatgttc catacttact ttggtctcta gatactcttg gtatactaac tcctacagat
     8761 tttttcataa cgtactgtgt gaatccagaa cagtcaaaac tatttggacc ttctgctccc
     8821 catacatatg gttttccaag tagtgtcttt gcaaaactta ttacactatc agctccttga
     8881 cttggagaat tagtcgaata agatggaatt gaagtatcaa cagaaccttt ttcagcaagg
     8941 tattgacttg acacatatcc agtttgattg ccataactta cttttgtcca tcctccactt
     9001 tcagaaatac ttccaacctc agtaccataa cttaatgtag ctattttaga atgacttgta
     9061 cctggtcctg ttctaacgtt caatgacttt gctgttacaa cctttgtacc ctttacaagg
     9121 tcatttgaac tgttttcagc attttcattt gtagagtcat ttacactagt tcctaaatat
     9181 ttacttgaaa cataacctac tctaccatta gaacttattt ttgaccatcc atttgactca
     9241 gatatgtatc cgacttcagt cccatatcct agtgttgata ttttagaact acttgtactt
     9301 ggtcctgttc taaaatttaa tccttttgct gttacaactt ttgtcgactt aactgaagta
     9361 tcactacttt cattgctact tccaaggcta tttattgcta cataatttcc atgcacataa
     9421 cctgtgtttc cattatattt aacttttacc caagaacctt cttttcctac gtactcaact
     9481 ttatcacctt tgtaaagttt ccccattgat tcatggtttg tacctggtcc ttttctaaaa
     9541 tttaccgcat tccctgttat tgttctgctt tctatactca cactctctac ttcagactca
     9601 ttagcatatg ctaaattccc catagtaggc attattatcg cagttgccat tatactggct
     9661 gctattctct tttgattaaa attcataact ttccactcat ctcctctttt ctactatctt
     9721 tatttcactt caaaatataa aagaaataat tgacactatt aaaatatcaa ttatttcttt
     9781 tatattaata ttattacaaa tttgtaacta tttgtctata gtaatatttg cttaccataa
     9841 attaccatgt ataaatttta atctttaata gcctgtaatt tagtaatata gccatctttt
     9901 acctttttta aaaatggcta tattttttaa cttttttgta actttttaaa atccagttga
     9961 gccaaaccca ccttttcctc tttcggagtc acttaattct tttacttctt ctatgttaat
    10021 atcatagata ggttttacta tcatttgagc aattttcata tgcttttcaa ccttaaaatc
    10081 atttttacca tgatttatta atataatttt aagctctcct ctgtatcctt cgtcaatagt
    10141 tcctggtgta tttaatacag taacagaatg ctttaaggca agcccacttc taggtcttac
    10201 ctgcgcttct gtcatagtag gaagctctat acatatccca gttttgataa gtttagtctc
    10261 tccaggaggt attacaacct cttctataga atacaaatcc ataccagcat cacctttgtg
    10321 agcaaaatta ggtattatag catcatcatt taattttttt acttttaaat tgtacatctt
    10381 atatctcctt tcaactttct ataaatatta taacttaaat ttcgacctct tgaagttttt
    10441 tatttttttg tggtaatata attattagtg ttttaaagga ggatttttta tgaaacttgt
    10501 acttgctgag aaaccatctg tggcaaaaac tatagcatca tttttaggtg caaagaccag
    10561 acaagatggt tactttgaag gaaatgatta tataattact tatgcagttg gacacttagt
    10621 atcattatat gatatgaagg attatgataa agataaatac tctggctctt ggaaaatgaa
    10681 caatttccca tttgttcctg aggataagtt taaatttaag attgatagtt ctaaaactaa
    10741 acaattcaac actgtaaaaa agcttttgaa tagaaaagat gtagaatatg taataaatgc
    10801 cactgacaat gaccgagaag gagaacttat agcattttta atatttttac tggcaaaaaa
    10861 taaaaaacca gtaaaaagaa tcttggtaaa tgaatggaca ccagaagata ttacaagagg
    10921 tataaaaaac ctaaaagatg aagatgaaat gagaaatctt caagctgctg gatatactag
    10981 gcttattact gactggttga ttggaataaa tttcacttct gttgctacat tgaaatatgg
    11041 aaatgggaaa ctattaaaca taggtagggt aattcttcca acagttaaat tagtctatga
    11101 tagagatatg gaaatactaa actttgttcc taaaacctat tatgaaatag aagggcattt
    11161 taaagctgaa gctggtgaat ataaaggtaa atatgttaag ggtaaagaaa gtaaatttga
    11221 tacacttgaa gatgcaaata aaattatagc ttcaattaca tctgaaactg gtaaaatact
    11281 agataaaaaa gttactatgt caaaggaata tgcaccaaaa ctttttagtt tgacatcact
    11341 tcaaggatat ataacttcta agtattccaa ttttacatct gataaggttt taaatgtctg
    11401 tcaatctctc tatgagggta atggtaaggg tggatacata acttacccta gaactgattc
    11461 cattttctta gaagaaagtt tggcttcaaa agcttctcaa actttagata aattaaaggt
    11521 tggtcttgaa tatgaaaata aaattaaatt ttctaaaact aaaagagttt ttgattcttc
    11581 taaagtagac tcccatagtg ctataattcc aacttatata attccaaaaa gtttatctaa
    11641 ggatgaacaa ttagtttatg atgccataaa agatagattt gtagcaaact ttatgcctcc
    11701 tgctgaatat gagaatacag aaattaagac tgaagtagat aattgtactt tcttaacaaa
    11761 aggtaaggtg cttaaatcaa aaggatacct tgaggtatat aataaggaag aaaaaaacga
    11821 tttacttcca cttgtaaata aagatgatgt tgtagatgtg ttagaaataa aaccattaac
    11881 taagcaaact actccaccta aaccatatac tgaggacaca cttttaaagg ctatgaaaaa
    11941 ttgtggtaaa aatgtgcctg aagaagatac aacagtgcta tctggatatt cgattggaac
    12001 atcagccaca cgtgctgatg tgttaaagaa gataagtcaa gtgggctatg taaagaaaaa
    12061 aggtaaatct tattctataa cagaccttgg gaaaaattta gttgaaattt ttcctgttaa
    12121 agacttattt gatgtagatt atactggaaa acttgaaaaa agtttaagcg atattcaaaa
    12181 aggaaagtat acaagaaaag aatatcttac taatatcatt agttttatat atcaaaatgt
    12241 caatattata aaacaagatg cttcaaaaaa tataaacaca gaagcatata ctttcgattc
    12301 aaaaactaaa aaatttacta aaacaaaaga aaaaaaacag aaaaaagatg taactaatac
    12361 tgaaaaagaa gttgctatat caaaggattc taataacaaa aaagaagtta aaaatggtga
    12421 tgaaaatcaa tctcttggca aatgccccat ttgtcaaggt gatgtactag aatttgacaa
    12481 aggctttgct tgtaaaaatc ataaagaatg taaatttgtt atttggaaaa atgataagta
    12541 tttaagtctt tataaaaaga aagttaacaa aacaatggtc aaaaatattt taaaaaaagg
    12601 tgaaactaaa gtaaaatctt taactgctaa aaacggtaat aaatttgatg ctacattaaa
    12661 atatgataaa aatccagata ctggctattt caattggaaa atagagtttg acaactaaat
    12721 attcataaga aaagaaatag cagtagtata ataatctata ttatattact gctatttctt
    12781 ttcttatgaa gttttaactt ttttaatagt gacttttaca atcacactct aacctaattt
    12841 ctttactatt tctgataact ttaatgtatg ttcatctaat aaagttagtt cctcttcaga
    12901 aagattagat aattttttta ctagagattc tttgagaata gcttttactg catcaaataa
    12961 tttaaatgct ttatctgtta attcaaccct aagaatcctt ctatcttttg ggtcaggaaa
    13021 tttattgact aaaccatgtt ccactaatct gtcaataatc ggagtcatat ttgattttaa
    13081 aattcctaaa ttagaagcta tttgagaaat tgatgatgaa tttgatgtca taagataaag
    13141 aattacctta gcatgtgatg gagccattgg acattcttca aaataagctt tcattatatc
    13201 agagttattt tgaaactttt ttaacaaatc gctttcttta aatatattat tttcaagcac
    13261 aaataaaaaa tctaaaaaag agtccgctat tttaagtata ttgttgttat ccatatttgt
    13321 aacatccctt caatagataa tataataaaa cattgttgtt taatatagta taaattataa
    13381 actattaata aatttaaaac aagaaaaagt tattgactca taataattat aaatttataa
    13441 tcaaattaga gaattataaa ttgccaataa agggaagttt tttaattagt ctaaaactaa
    13501 acttaaatta taagacactt taaattctac aacaagctag tgtgcaatat ataataggag
    13561 gagatatgaa aagatattta atagttttct taatgtgtag ttcactgttt ttagttggat
    13621 gtggaaaaac agaagaatct gaacctgaga aaccaatagc agtttctgtt caaaaagcaa
    13681 taggtggaga aattgaaaac acaaattctt tctcaggaac tacaaaggtt aaagatgaaa
    13741 cagctgttac tgcacagact gttggcacag ttcaagaagt gtatgtaaaa cttgggcaaa
    13801 atgtaagaaa aggagatgaa ttattgagta taagcagtcc agagctagaa aatagtgtaa
    13861 agcaaagtaa agcctcacta gatttagcaa aagcaagtta ttcaagtgcc actggtggtt
    13921 cattagaggc acaagtaaat caggcaaaaa cagctcttga caatgcaaag attcaatatg
    13981 aggagtctca aagaaattat gacaataata agattcttta tgaacaggaa gtcattagtt
    14041 tagaccaatt taaaaaaata gaattttctt tagagcaaac aaaacaacag ttagattctg
    14101 ctcaaagagc atacgataca gcaacttcaa aaagtatacc tcaagctaaa gcacttgcta
    14161 aaaaacagct tgaccaagct caagtatcat ataatttagc aatgagtaat ttagacaaat
    14221 taacattaac atctccagta gatggtacaa taacagctaa aaactttgat tcaaaggaaa
    14281 tgataacaca atcacaacca gcttttatta tttctaatcc tagtatacta gaagttgatt
    14341 taaatgtagc agaatcagat ataggtaaat ttaagaaaga tggaaatgta gatgttataa
    14401 ttgaagacca aaggatttta ggaaaaatag attatgtacc ttcagtagtt gaccctcaga
    14461 catctttata tcctgttaaa gtacttgtaa ataatacaaa caacaaattt aaggcaggta
    14521 tgtctgctca agtaaactta agtgttgaaa aagaaaatgg tgctgttaca gtacctaaaa
    14581 aagcaatatt tgaagaaaat ggtaaaaaat atgtatacat agcaacaaaa gacaatatag
    14641 caaagaaaca cttggttcaa actggaattg taacagagga taaaatagaa attaaaagtg
    14701 gtgtaagtga taaagataca gtgattattg gagggataag tcttatttct gacggaacta
    14761 agatatttcc tgtagaaaag gagaagtaga ctatgaattt aacacagact tccgttaaac
    14821 gtcctctcac aatcataatg gtctttttag tattaatagt ttttggtggt attggatata
    14881 aaaaaatgtc aataaatcta atgcctgata tagaaatacc agttgtaatg gtcatgacta
    14941 cttggacagg tgctggtcct caagacgttg atgaacaagt tagtcaaaaa gttgatgaaa
    15001 gtttatctgc cgtttcaaat gtaaaatcta ctatatcaag ttcacaagaa agtgtttcta
    15061 tggtagttgc tcagtttgaa tttggaacta atttggatga aataatgaat gatgttaggt
    15121 ctaaagttga tgcattacag accagcctcc cagatgatgc agctaaacca acagttttaa
    15181 agctggatat gaatgcacag gccataggtc aacttgtcat atctggagga aatgaaaata
    15241 gttcacaagc cttaagaaaa tacgctgaag atgtaattca gcctaaaata gaatccatag
    15301 atggtgttac ttcagcagac cttaaaggtg gaaaaaaagc acaggttaat gtaatagctg
    15361 acccagctgt tctttcaaat tatggggtta gtttatccac tataaaaggt gtcttatcct
    15421 ctagtaataa aacattccct tatggttcaa ttactcaagg tgaggataaa atagttttaa
    15481 gagcgattga taaattagaa tccctagatg atataaaaca gattcaaatt cctgtaaaag
    15541 gaggaaatac agttagatta gataatcttt gtagtgtaga ttatggatat gcagacaaag
    15601 atagtattta tagatacaat ggaaaagaca gtttggtaat agatatttct aaacaacaag
    15661 atgccaacac tgtaaaagtt atgcagagtg catacaaata cgtagatgaa ttgaataagg
    15721 aaaatcctca atttaaaatt aaaattgcta atgatacaag ttcatacata agtgaatcaa
    15781 taaacagtgt aatgagcaac ttgcttataa gtgctgttat cgcatttatt gtaatatttg
    15841 ctttcctaaa aagtgtaaga gcttcactag ttgttgcagt tgcaatacca atttctataa
    15901 taggtgctat agcaattctc tactttactg gtgaaagttt aaatcttatt actgcaagtt
    15961 cattggtaat ttcagttggt atggttgttg ataatgctac agttgtaatt gaaaatatat
    16021 ttaaatatag gaaaagtggc agattgagtc ttgatgattg tgctattgag ggaacaagaa
    16081 ctgtaacaaa tgcaatactt gcatcaacat tgaccacaat agcaatattc ttacctatca
    16141 tgtttacaga aggtatagct aaaataactt ttggggcatt aggtaaaaca ctaatagctt
    16201 cactagcatt ctcctttata ggtgcaatta cactcgttcc tagtgtattt gcaaaattaa
    16261 gcagaggaaa aaatagtcaa aagatgcaag aaaaagatag tcctatattt gataaggtaa
    16321 gtgaagcata taaaaaatta ttaagtgtat gtttgagaca tcaacgctta gttgtaatat
    16381 caagtttact tatttttgta gcttccttat ttggaatggg atttataggt atggacttta
    16441 tgcctgcagc agacaagggt gaattatcta tttctataga acttccaaaa ggtcttagcc
    16501 tagaatccaa tgattactat gtatctatga ctgagaaaaa agtctcagat attccagaaa
    16561 ttaaaacttt aataacaact ttgacatcag attcaaataa tagtaaagca agtatagcaa
    16621 ttgaattaga atctcaaaaa aatagaaaaa gaactactga tgaaatagaa aaagagataa
    16681 ttgatagagt ggctactgta ccagattgta aaataaatgt aagtcaaaac agtagtgtta
    16741 tgggtggagc tggaggagca gacttcacat tagaaatgaa aggtcctgat ttagatgtat
    16801 taaaaatact gggaaaagaa gctgaaaaag aatttagctc tataaaagga tttagaaatg
    16861 ttgaaacttc tcttacagat acaacacaag aagctcaatt tactatagat aaacataagg
    16921 ctcaagaata tggtgtaaat accgctgaaa ttgcaggcat gttacgtaca gcaataaatg
    16981 gtgactccgt aaccactgct actattgatg attacaaagt ggatgtaaac ctaaaattta
    17041 aagcagatac tattgatagc ttagaagata taaagcaact aaaagtaaac tctgcaactg
    17101 gaaaagaagt tcctcttggt gctttttcag atataaaaat ggcagatggt ctacaatcta
    17161 tatctaggac tgatggagat ttttctgtaa atataactgc tacacttgac aatttagatg
    17221 ctggtactgc tacaaaacta gcactagaaa aaacgaataa aatggtaatt cctaaagatt
    17281 actctatagg tgttggtggt gaaacagaaa tgatgaatga atctatggca agtcttggat
    17341 tttcaatggt catagctata gcattagttt atatggtaat ggtagctcag tttgaatcat
    17401 ttagtaaacc atttataatc atgttctgtg ttccatttgc attcgttgga gttgtgcttt
    17461 cgcttctagt tagtggtcaa agtttaagta ctgtcggtat gctaggtata atcttactta
    17521 tcggtattgt tgttaataat ggtatagttc ttattgacta catagagcaa cttagaaaaa
    17581 caaatcgtaa caatgattta attggtttgg tttcaattgg ttgtgcagca agattaagac
    17641 cagtcttaat gactacagca acaacaattt taggtatgtt gccaacagca ctatctcttg
    17701 gtgaaggtgg agaaactatg caaccactag ccatagttat aatgggtggt ctaactgtat
    17761 ctacacttat aactttggtg cttataccta ctatatactt aatatttgat aaatgggaaa
    17821 ataagttttc agacaaaatg cctaaaatat ttggtaagat aacagataaa ataggaagta
    17881 taaaacgtaa aataataaag aaaccaaaat tgaaagctaa agtttaaata gaatggaggg
    17941 tgattgtatt gaataaaaaa atgagcaaaa tagttgcaat tggctgtggt gttggtataa
    18001 taactgccaa tttaatccca gtttacgcag ataataatac ttcttctata aacaaagttg
    18061 ataattctat tttaagtgac tcaaataaaa gaacatctga aaaggaaatt ttgactgcta
    18121 gagaagctgt tagtgctgca cttaaaaata gtgaaaaatt aaaaatgaaa tcagaagaaa
    18181 taaaaatgtt aaaagaaaag ttagaagtac aagatgagtt tgatagtttt actggaagtg
    18241 acaactcgtt tccatacgac caaattggac ttcttaaaaa tcaaagtgaa caggctaaag
    18301 gctttatgga ggaccaaata gcaaatgata ttactaataa atttaacgat ttagtttcaa
    18361 gagaaaatga gctagacaag ataaaaaaca atcttgaaat aaaaactaaa gaaattaaag
    18421 atatgaaatt aaaaaaagat ttagggttag taacatcttt agaaactgaa tcagctgaac
    18481 ttgaacttca aacactacaa aatactcaaa aagctaaact tcaagaacta aaaaacaatc
    18541 aagactactt taagttgttg actaatatag atttaaataa ttatcaattg gataaagaat
    18601 atagatttga atcttttaga gtaggtggct cagttgattc atatatggaa ggtaaggtta
    18661 atgaatattt aaaatacgac caattgattt tagaacgtac agaagaaagc tttaatgata
    18721 aagatgagaa caaagcagat ttaccagata gacctaatct tacaagacca gttgctccta
    18781 caaaaccaga gcaaggtaac ctaactgatg atgaatataa ggttcttacg gataagtatg
    18841 aaaaagacta caagagatat gaaaaagaag ttgaagcata caatcttgaa agacagactt
    18901 atgctgctgg ccttactact tatgcaaatt atcttcaaca aaaatttaat accgaaaatg
    18961 gtctagtaac attagaagac agtaaaaaag ctcttaaaaa agggttaatt gatagctatg
    19021 ctcaattact tgctttagaa gatacaattc agattactaa aaaacaactt gatttatcag
    19081 aaaaacaatt aaaaaatact aaattaagat atgatttagg attaattact ctaactgatt
    19141 ataaaaaaca agtggtaagt aatgaagatg ctaaaaatag ctatgacact cttattgtga
    19201 attataactc actaaaaaat ggtatagaaa aaccttggat acttaatact ggaaaataat
    19261 tctggtaaat agaaagatta ctttaacata cgtttgatta aaaaatagtt atgcacactc
    19321 gtggctttag tcatggaaga ttcagaaggc aaataataga aatagattag taaaaatata
    19381 tttgcttata aaactgtata attttataga ggctatctca aaatagagat aagcctcttt
    19441 tttattgttt aaattataaa aatatattta attccataca aaaagagtgc cttatgaact
    19501 aaaaagttca ttttgagaca ccctcaaaag cttaactcta aatttaatta ttttttatca
    19561 aatgtctcat ctattttaca tctcaccatg ttaacaaatt cagaaatatt atatcctcta
    19621 agtttgcatc cacatttagg acaataatcc aatttcctag ataccaaagc cctacaacta
    19681 ggttctggac atcttatttt tagactctca tctgctactt taaatccaca tcgtgggcat
    19741 atactacctg gacgtgatga tacttccttt ccacatttag gacactttgt caactttatc
    19801 ccctccagta aactcaattt aattgatagt tgaaatttct ctctacaata atatatgcaa
    19861 tataattaaa tacgtttcac ttacaactat ataagttaaa tttttataat aaaaatcccc
    19921 tatattcaat ttacaaatac ttaatttgat acattgaaca ataggggatt ttatttttat
    19981 ttagttacat attttgcagt ctattattat ctaggatttt ttatagatgc ttgagctgct
    20041 gcaagtctag caattggcac tctaaatggt gaacaagata catagttaag tcctacacta
    20101 taacagaact ctactgaaga tggttctcct ccatgctctc cacatattcc tagttttaat
    20161 tctggtttaa cttctctacc taatttagct cccatcttaa ctaacttacc aacaccattt
    20221 tggtctagta cttggaatgg gtctttttct aatatttctt catctacata ttgacctaag
    20281 aatttaccag catcatctct tgaatatcca aatgccattt gagttaagtc attagtacca
    20341 aaactgaaga agtctgcttc ttgtgctatt tcatcagcag ttaaacaagc tctaggtatt
    20401 tctatcatag tacctacagt ataaggaact tctatacctt cttcttttat tatagcgtca
    20461 acagtttcta ctatcatttt tcttattatt tttaactcat ttacttctcc aactaatgga
    20521 accattatct ctggagaaac tttaacacct gctttgattg cttctatagc accttgtatt
    20581 atagctttag attgcattac acatatttca ggataagtta ttgcaagtct acaacctctg
    20641 tgtccaagca ttgggttaaa ctcatctaaa tcaactattc tcttcttaat atcacttact
    20701 tttattccca tagattttga aagttcttct atagtctcat catcatgagg caaaaattca
    20761 tgtagtggtg ggtctagtaa tcttatatta actggttttc catccataac tttaaataac
    20821 tctacaaaat cttctctttg cattggtaat attttttcaa gagctttttc tctttgttct
    20881 actgtattag ataatatcat ttctctaact gctggcaatc tagcttcatc aaagaacata
    20941 tgctcagttc tacatagacc tataccttct gcaccaaact ctatagctgc acgagcatct
    21001 ctaggattat ctgcattagt tctaaccatc atacatttgt gtttgtctac ccagttcatt
    21061 aatttttcaa aatttccagt taaagatact tctgttttct taacatctcc taggtataca
    21121 tttcctgtag aaccatctat agaaatgtat tctccttctt ttataactac atcatcaagt
    21181 gctcttattt ctttattaaa ctcatcaact tttatttctc cacatccagc aacacaacat
    21241 ttacccatac ctctagcaac aactgctgca tgtgatgtca ttccacctct agctgtaagg
    21301 attccttctg ctttaaccat tccttctata tcttctggag atgtctctag tctaactaaa
    21361 actacttttt ctcctttttc atttgctttt actacatcat cagcattgaa ataaacttta
    21421 ccacttgctg cacctggtga tgctggtaaa ccttttgcta taaccttagc ttcttttaat
    21481 gccttatctt caaattttgg atgtaataat tggtctaatt gattaggctc aattctcatg
    21541 atagcttctt tttcatcaat tattccagct tcaactaaat caaccgctac atttattgct
    21601 gctgctgctg ttctcttacc atttctagtt tgtagtatat ataatctttc attttctata
    21661 gtaaactcta tatcttgcat atctttataa tgaccttcaa gtatatgagt aatttttaca
    21721 aattcatcga atacagctgg cattacatct gcaagagttg atatattttg aggagttctt
    21781 atacctgcaa caacgtcttc accttgtgca ttcattaaaa attcaccaaa taatttattt
    21841 tctcctgtag ctggatttct agtaaatgca acacctgtac cactagtttc tcccatatta
    21901 ccaaatacca ttgattgaat atttactgct gttcctaagt tatgagctat atcattcaat
    21961 ttacggtata cgattgcacg aggattattc catgatctaa atacagcctc tattgcaagc
    22021 attaattgct cttttgggtc ttgtgggaaa tcatttttta tttctttttt ataaattgct
    22081 ttaaattctt ttactatttc ttttaaatca tcagttgtaa gttctgtatc aactgtgtat
    22141 ccctttgctt cttttactct gtctaataca ttttcaaatt tatattttgg aacttccata
    22201 gcaacatcag aaaacatttg tatgaatctt ctgtaactat catatgcaaa tctttcattt
    22261 tgtgtagcct ctgctaagcc aacaacacta ttgtcattaa gacctaggtt aagaattgta
    22321 tccatcattc ctggcataga gaatactgca ccagaacgaa ctgatactaa taatggattc
    22381 ttattgcatc ctaatgtttt atttaagtct ttttctaaag tagctaaatg ctcttctatt
    22441 tcttttatta tctctgctct tatactttca ttgttaacat aataatcatt acatgcttct
    22501 gttgttatag tgaatccagg agggacaggt aagcctatct tagtcatttc tgctaaattg
    22561 gcacctttac ctcctagtaa agatttcatg tctttgcttc cttcaccaaa actataaaca
    22621 tacttagttt ccattgttaa atcccccatt aatcttttta ttttaattta tatcattttt
    22681 attaatagac attattaaat ttctatttct ttgtatatct ttagaccatt ttctttcatt
    22741 atgtctaaaa ttatacctgc tgtttcttct attgccttat ttgaaacgtt tattactggg
    22801 catccaattc ttttcattat ctcttctgaa taatctaact cctgaagtat tctttctaaa
    22861 ttagcatagt ttgctttact ggacaatcct aaagctttta atctttgggt tcttatctca
    22921 ttcagctttt caggtgaatt agtaagacct attatctttt tagtatctat ctcaaacact
    22981 tctttaggaa ttggtatttc tggaactaat ggaacattag ctacttttat atttttatta
    23041 gctaaataca tactcaaagg agttttagat gttcttgata ttcccactaa aattatgtct
    23101 gcttgaagaa cacctctagg gtcttttcca tcatcatatt tcactgcaaa ttctatagct
    23161 tcaactctct taaagtaaga ttcatctaat tttctaatta taccagcttc tcttttaggt
    23221 tttcttcctg ttctcttgct tatttctctt agtatatctg tcattaaatc tatacaacta
    23281 agaccttctc tttcgcaata tctttcaaca atactgcata attcttcatc aactaatgta
    23341 tatacaatta aagcgttttc agcttttgca ttctccaaag tttcaagtag tactccttta
    23401 ttaatcatgt atggaaatct ttttatttca taagtttcat tcacataaaa ctgagacata
    23461 catgcctttg caacttgttg tgcagtctcc cctactgaat ctgatactgc atatataatt
    23521 aggtttttca ccagcatacc tccttaattg tcaactattt ctaagaatag cttagttatg
    23581 ttggtttttg atattctccc aacaactctc atatgatttt tgtcttctcc cttatattca
    23641 acaatcggaa tcgaatctac ttcatgttct attatttttc ttgaagctaa taaaacactt
    23701 gctcctttag ttaaagtgac tacatttgga gttcttgtca ttatcatacc tattggcatt
    23761 ttatttatat cagaacctcc tatagttgct tttagcaagt cttttctgga aacaactcca
    23821 cataactctt cattctcatc tataataact atactcccta catcagataa aaacatagtg
    23881 actattacat catatatact ttcatccttc ttagctagta caggcatgct cattatatcc
    23941 tctactgttt tttcttttac acttttacca attaaattta tttcactaac tcctgaataa
    24001 aaatatccaa cttttggtct tgcgtctaat attcctgtca ttgttaatat agctaaatca
    24061 gatctaagtg tagcacgagt cacattcaag ctggaggcaa tactttcact cgttattggt
    24121 tcattttcct taactatatc tataattttt agttgtcttt cattaagctg aataataatc
    24181 accttctttc attgtgctat actttttatc ttttcgcatc ttatatagta tattatataa
    24241 ctatatatac cattctgtaa acagattttt tattgaatac gatatatttt ttactaagtt
    24301 ctctcgctta aagatattta ttatatatta atgggggata tttctttaat taatagaact
    24361 atattagtat tacagttaat attataatac ataattggtc attttaattc attttatatt
    24421 aaaattttta ataatattat tttaggaggt aaaaatgata aacgatatta aaaataaatt
    24481 caaaaattac aaaccatata taaatggata caagaatatg aagagagcat ctgttttaat
    24541 tccaatagta gaaataaata acacacacta tatattattt gaagttcgct caaaaaattt
    24601 gaagcatcaa cctagcgaaa tttcttttcc aggtggtaaa atagaatcag gtgagactcc
    24661 atatgaagct gttattagag aaacttgtga ggaacttgga acttttagtg acaatataga
    24721 agtaatatca agtcttgacc tccttataac accagtaaag tttatcatac atccatactt
    24781 aggatatata aaaaatatta ataatttaaa tataaacaag gatgaagttg accatacttt
    24841 tttggttcct ataaaatatc ttttagagaa tcctccaaat acttatagta atactgtaaa
    24901 agtttttcca gatgaaaatc taccatatgg ttcaattcca aaccaaaaag attataaatt
    24961 tgaaagtgga agttatccta ttctgtttta taaatataaa gattatgtaa tttggggaat
    25021 gacagcaaaa atactagaaa actttttaaa ttttttatca gaataacact cataataaat
    25081 tatcaaaatt tttacataag aaatttataa aagtttctaa atttaacaat tctaaaatat
    25141 caataactat attttgtagc actttttta
//
LOCUS       DAYAPA010000051        24527 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.051, whole genome shotgun sequence.
ACCESSION   DAYAPA010000051 DAYAPA010000000
VERSION     DAYAPA010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24527
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.051"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            405..3059
                     /gene="adhE"
                     /locus_tag="ACGI59_001925"
                     /gene_synonym="adhC"
     CDS             405..3059
                     /gene="adhE"
                     /locus_tag="ACGI59_001925"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFG9739711.1"
                     /translation="MAHKEQVIIENKKENEVMSVGLDTVNNVESLVKKLAKIREAQKI
                     FATYSQEQVDKIFLAASLAANKQRVPLAIMAQKETGMGIAEDKVIKNHYASEYIYNAY
                     KETKTCGVIEKDEAFGMTKIAEPIGVIAAVVPTTNPTSTAIFKALLALKTRNGIIFSP
                     HPRAKNSTIEAAKVVLEAAVLAGAPEGIIGWIDEPSLELTTTVMKEVDLTLATGGPGM
                     VKSAYSSGKPAIGVGAGNTPAIIDDSADIKTAVNSILVSKTFDNGMICASEQSVIVLE
                     NIYNEVKKEFKERGAYLLDKDETEKIRNIILVNGGLNSKIVGQTACTIAKLAGFEVPV
                     DTKVLIGEVESVEIEEAFAHEKLSPVLAMYKASDFDDAVRKAEKLVEDGGFGHTSSLY
                     IDDVNQREKLDKFTSAMKTCRILINTPSSQGGIGDLYNFKLAPSLTLGCGSWGGNSVS
                     ENVGVKHLLNIKTVAERRENMLWFRAPEKVYFKKGCLGVALKELKDVMNKKRAFIVTD
                     TFLYNNGYTKAVTDLLDEMNIKYTTFFEVEPDPTLECAKIGAKAMREFNPDVIISIGG
                     GSAMDAGKIMWTLYEHPDVDFQDLAMRFMDIRKRVYTFPKMGEKANFVAIPTSAGTGS
                     EVTPFAVITDQDTGVKYPLADYELMPNMAIVDSDMMMNMPKSLTSASGIDALTHALEA
                     YVSMLATDYTNGLALQAIKSIFEYLPRAYDNGAKDPEAREKMANASTMAGMAFANAFL
                     GVCHSMAHKLGAFHHVPHGVANALLITEVMKFNSSDAPKKMGAFSQYKYPEALKRYAE
                     IASFLGLKGNSDEEKFQSLLVAIEDLKLKVGIPKSIKEFGVEESKFMDSIDEMVIQAF
                     DDQCTGANPRYPLMNEIKDMYLNSYYGR"
     gene            3335..5554
                     /locus_tag="ACGI59_001926"
     CDS             3335..5554
                     /locus_tag="ACGI59_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFG9739712.1"
                     /translation="MTGRNKSFTLITILFLTSLFLTMGYIYIEDTKHLLMSEAKIHIK
                     EVAVQGSQLAQRQIEKDLDVLNIFSNYYASNPDIPNEEKMKNLLDEMENQKFYTMAIV
                     DINGNAENTKGDKFSVKDREFFKNSIKGKKYVSSPYVDEVNKSIKKIAISVPLLNNDK
                     VVGVLYCTYNINTLMKLINISFYENNSISYVVKNNGTIILHPQGDSLSKNIYKLLKQD
                     NDIQEVNRLKKELQENKTGATVLNMLEERRYLGYATMDNGNSENNYIKDWNLIFSIPE
                     TVVFSNSKQIINRAVYAVLSIVLIFIAIIFYIIYIKKSNEKEILSLAYEDKVTYIGNQ
                     NKFYRECSKYLLDKPSLNYIIVYFDINNFKMINDTFGYEFGDNLLITIAKALKEELTE
                     GEVYARLSSDYFAIFCDYKNGRNKIIRKLDNIRSNIESNLSIVFEISLCVGIYFVEEG
                     EVDIQKAVNKANMARSVAKGKNINYAIYNEDVRNKLSEESMILDDIKIALVKNQFEVY
                     YQPKFSLVTGEMIGSEALIRWNHPEHGFISPAVFIPIAEKSKLILKIGRFVFERVCND
                     LYEWKKQGKKIVPVSVNLSRVELYQPDIVKFINKTIKMYNLSSDFIEIEITETVAINE
                     LNILKNVLNELRTYGFSISMDDFGTGYSSISCLRDMPIDILKLDKSFLGGIEHDERSR
                     NIAKSIVSLAKSLDLVVIIEGVESKEQAELMKQFGCDLVQGFYFARPMPAKNFLDLL"
     gene            5808..6470
                     /locus_tag="ACGI59_001927"
     CDS             5808..6470
                     /locus_tag="ACGI59_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HFG9739713.1"
                     /translation="MIDISIDDKLKERWPNAKLGCIQAKVAVNKSSEKLINEINEFCD
                     TINQSLKVEDITKLDKIKDARNAYKELGKNPSRYRTSSEALVRRIIQGKGLYTINNIV
                     EINNLISIKSLYPVGTYNVSKLHSPVCFTVGDEGEQYKGIGKELINIENLPILSDSVG
                     KFGSPTSDSERAMITEDANEILICIFSFSQESDIEEYFGYAKELLKEYADGKDIETKI
                     IK"
     gene            6841..8250
                     /locus_tag="ACGI59_001928"
     CDS             6841..8250
                     /locus_tag="ACGI59_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HFG9739714.1"
                     /translation="MIDGKEVKQESGQKNLKFKIAIVVGVLFVAYIGMAIFFRNHFYF
                     GTTINGVKASGKTVEQVNDLLTSSTNSYTLNLEERNNKKEQIKAKDIDLKFTPDDKVQ
                     KIKDSQSAIGWIGGIFKSKEYNNMITLSYNKDLLEKRFNALSCFDSKNVVEPKSAYPK
                     YDSTKNAYVIVPEVLGNKVKKDDFYKLVQNSINSGNTSISLDESKVYEAPNNTKDSEK
                     LKQAIKTLDKYVGVVVTYDFSDRKETLDGSEINKWLKVDSEKNYDITFDNDAMVEYTR
                     GLSKKYSTFGDSRPFKTATGKDITISGGIYGWLIDKKKEAEALVDVVKAGKNVTREPI
                     YIQKAVSRNKDDIGNTYVEISLSGQHMWFFKNGEMLVSTDVVTGDLARGFATPSGIYP
                     LNYKARNVSLTGQGYSSPVSYWLPFNGNIGIHDATWRNSFGGSIYKSSGSHGCVNTPF
                     SKAKKIYENIEPGTPIILY"
     gene            8365..8640
                     /locus_tag="ACGI59_001929"
     CDS             8365..8640
                     /locus_tag="ACGI59_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif11-like leader peptide family natural product
                     precursor"
                     /protein_id="HFG9739715.1"
                     /translation="MNEKLTKILEEAKNDDEFRKELIKTREQRDVMDSFCKVVTSRGY
                     DMTVGELFSMGEEYTSNLLKSVNGGAAYPIEDWSDWYEDFFIMLSNI"
     gene            9270..9581
                     /locus_tag="ACGI59_001930"
     CDS             9270..9581
                     /locus_tag="ACGI59_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9739716.1"
                     /translation="MAFEIIKSIVEAEQTADSIKVKAVTDAESIRADAVNKCESIFAD
                     VKKQAKLMEETLIEKAVTDSRAEVDKILANAKSECLKIEKTAEERKSKAIEAVIGKVV
                     R"
     gene            9583..11508
                     /locus_tag="ACGI59_001931"
     CDS             9583..11508
                     /locus_tag="ACGI59_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HFG9739717.1"
                     /translation="MAIVNMNKISIVGLEAQKSQILKLLMNRGFVQIDDSAFLTEEEE
                     LKNYLVKDSEESEVIMLEQKMSHVNQAINIVSSLVKQKKSLLAPKREFNKIDSEKEGQ
                     VYAEALELNNLHKEIGTIRSNINLLNNNKSMLEPWVNFDIPLENMETKYTKTILGTVP
                     ISANIDALRVKLEDEIPESVIGIVNSDKLMNYVYLITLKETFDDVLEVLKEFNFSVVS
                     LPNEEGTPLQAIKKYNEMIIRQENVSKDKTEEIKRHADSIHMLENLYDFYTIERDQKK
                     IIERLVKTKTTFCLNGWLPSKRADELIKEITEKFNCCVQTEEGSKEEGFPILLENNKL
                     VTPFESVTNMYSYPSTKDIDPNTILTFFFVVFFGMMLSDAAYGLIIAVVCGFVVYKLK
                     IQKGEGNLIKLIGICGVSTTVWGLIFGGILGDLIPIKALINPLEDVMTLMGMSLLFGI
                     IHIYVGLGIKAYTLIRSGKVIDAIFDIGFWYLCITGVCLLIIPFVAGDIGVFSEVGKY
                     LAIIGAIGLVLTQGRSFKGIPVKLFKGFSSLYGITSYFADILSYTRIMALCLTTGVIA
                     QVINLLGAIAGPILAVVIGVVGHTINLLINALGAYVHTSRLQYVEFFNKFYEGGGVPF
                     VPFKYKTKYTSINKKEM"
     gene            11511..12002
                     /locus_tag="ACGI59_001932"
     CDS             11511..12002
                     /locus_tag="ACGI59_001932"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HFG9739718.1"
                     /translation="MEFLNALGNGQFLAISGAAIAALFAGMGSAKGTSIAGQAAAGVV
                     TEDPSKFGQLLVLQLLPGTQGLYGFIIAFLILSKAGIIGGDAIPTSAQGLQLLMAGLP
                     IGLVGLVSGIAQGKAAAAGVGIVAKRPEELGKAITFAAIVETYALLGLLVSFLAYNGI
                     AIG"
     gene            12054..12617
                     /locus_tag="ACGI59_001933"
     CDS             12054..12617
                     /locus_tag="ACGI59_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HFG9739719.1"
                     /translation="MGNEQKMIDRIIADAKQEAQEILDKAKSEADLKVNSANEKAEKE
                     MASYTKLAEAEAEKAASKEISGAYMEAKKQILSKKQEILEEVILEAKNKLLNLKDNEY
                     EEIILNMIEKSNCTDDSEIVLSKKDKKTLKDVLSKKGIKVSDETRDITGGFIVKKGDI
                     EYNYSFEAIIAVEHEYIEQIAAEILFN"
     gene            12632..13609
                     /locus_tag="ACGI59_001934"
     CDS             12632..13609
                     /locus_tag="ACGI59_001934"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit C"
                     /protein_id="HFG9739720.1"
                     /translation="MAEEKTYPYAVARIRVLEKQLLNRQMFIQMAEAKSPEDSLRIIA
                     EAGYADTSNNNIHNFENILTQELSKTYEILKSLAPEEKFVDVFLYKNDYHNLKVLIKE
                     EISGVDGEKYLIDGGTIPLIKLRESLANRSFSDLPKIMSSAILEAFDVYNKTQNGQMV
                     DIVLDKATFTSMKETAKESQNKFVIDYVMKVCDLTNLKSFIRVKNMKKDFDMFMNVFV
                     SGGSLDKEKFLEAFSSDTPASCFKSTSYSDVCKNGMDSGFTVFEKLCDDYLMEYVRGA
                     KFKPLTLEPLIAYLYAKESEIKTIRIILTSKLNNIDADTIKERLRDAYV"
     gene            13602..13919
                     /locus_tag="ACGI59_001935"
     CDS             13602..13919
                     /locus_tag="ACGI59_001935"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HFG9739721.1"
                     /translation="MYKVGVVGDKDSIMGFLALGIDIFPAYDSDEIKKSIHKLVEDEY
                     AIIYITEQASLLAKESIAKYKDYQLPAIIVIPGIGGSMGLGMNEVRESSKRAIGADIL
                     FKE"
     gene            14036..15814
                     /locus_tag="ACGI59_001936"
     CDS             14036..15814
                     /locus_tag="ACGI59_001936"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HFG9739722.1"
                     /translation="MKTGTIVKVSGPLVVAEGMRDANMFDVVRVSDKHLIGEIIEMHG
                     DKASIQVYEETSGLGPGEEVVSLGMPMSVELGPGLISTIYDGIQRPLEKMYDISGTNI
                     TKGVEVSSLDRTTKWEFKPKKSVGDKVVAGDIIGGVQETAVVECKIMVPYGVKGTIKE
                     IYSGEFTVEDTVCVITDEKGNDIPVTMMQKWPVRKERPYREKKTPDSLLVTGQRVIDT
                     FFPITKGGVAAIPGPFGSGKTVTQHQLAKWADADIVVYIGCGERGNEMTDVLLEFPEL
                     KDPRTGESLMQRTVLIANTSDMPVAAREASIYTGITIAEYFRDMGYSVALMADSTSRW
                     AEALREMSGRLEEMPGEEGYPAYLGSRLAQFYERAGKVNCLGMDEREGTLTAIGAVSP
                     PGGDTSEPVSQATLRIVKVFWGLDASLAYKRHFPAINWLTSYSLYQEKVDVWADKNVN
                     DNFSAQRAQAMKLLQVESELQEIVQLVGVDSLSDGDRLILEVTRSIREDFLHQNSFHE
                     VDTYTSLHKQYRMMGLILKFYKSAQDAIKQNVTINDIIKLSVLEKIGRAKYVEEGDID
                     AAYDSIEDEIDNELADLIKKRGAEEL"
     gene            15811..17184
                     /locus_tag="ACGI59_001937"
     CDS             15811..17184
                     /locus_tag="ACGI59_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HFG9739723.1"
                     /translation="MIKEYKSIQEVAGPLMLINGVEGVKFDELGEIELSNGEIRRCKV
                     LEVNGDTALVQLFESSTGINLAESKVRFLGKSLDFGVSPDILGRVFSGMGRPIDGGPE
                     IIPDKRLDINGAPINPAARDYPAEFIQTGVSAIDGLNTLVRGQKLPIFSGSGLPHANL
                     AVQIARQAKVRGTDSKFAVVFAAVGITFEDAEFFISDFKATGAIDRSVVFVNLANDPA
                     VERVSTPRMALTAAEYLAFEQDMHVLVIITDITNYAEALREVSAAKKEVPGRRGYPGY
                     LYTDLATMYERAGKMKGHEGSITMIPILTMPEDDKTHPIPDLTGYITEGQIILSRELY
                     KKDIQPPIDVLPSLSRLKDKGIGKGKTREDHADTMNQLFAAYSRGKDAKELMAILGES
                     ALSEIDLMYAKFADEFEKEYVSQGFRTDRTIEQTLEIGWKLLSMLPKSELKRIRDEYF
                     EKYMPKE"
     gene            17190..17858
                     /locus_tag="ACGI59_001938"
     CDS             17190..17858
                     /locus_tag="ACGI59_001938"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HFG9739724.1"
                     /translation="MARLNINPTRMEMTRLKKLLKTATRGHKLLKDKLDELMKQFLEI
                     VRENKRLREEAENALDTAYKNFIIARAVMSQEYLGSALMMPKQSVSVDVSTRNIMSVD
                     VPVFDFKTENNQSDIYPYGLAFTSGELDSAMEAFSDAMQPLLRLAESEKSAQLLAQEI
                     EKTRRRVNALENVMIPNYIETIKYIAMKLEENERASTTRLMKVKDMVLKKALEEKKKN
                     DLVV"
     gene            18135..19184
                     /locus_tag="ACGI59_001939"
     CDS             18135..19184
                     /locus_tag="ACGI59_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033225 - ATPase-coupled
                     2-aminoethylphosphonate transporter activity [Evidence
                     IEA];
                     GO_function: GO:0033226 - 2-aminoethylphosphonate binding
                     [Evidence IEA];
                     GO_process: GO:0033223 - 2-aminoethylphosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HFG9739725.1"
                     /translation="MKEFIFTCLCIVVITFMTSCISTTPQTTQGKGNNKLTIYAALEE
                     EQIAYYMEGFREEYPNIDVEIVMDSHGVIASKLLAEKDNPQADIIWGLSAMNMIELKK
                     DGALEAYKPKNYDKVDKKFKDTEDDVSWVGMSVPETAIVVNYKELKKLGIDIPKSYED
                     LIKPEYKGLITMPNPASSGTGYLTVSGLIQMMGEKDAYNYMDKLHKNIATYTHSGSKP
                     AKLAGSGEYPIGITLGYRATQQLSSGEPIEVVFPKEGSGWDLEANALVKKDNIKKEAK
                     LFLDWAISDSAMDRYAHEVAVTSIKTDNPVPKGYSKQPFEQLIKEVDLNSAAKNRSTV
                     LKTWESKYGSKSESK"
     gene            19280..19426
                     /locus_tag="ACGI59_001940"
     CDS             19280..19426
                     /locus_tag="ACGI59_001940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9739726.1"
                     /translation="MSYLKINNVFKSYDQKRVLNNISLDIEEGEFLCLLGPSGYYMLV
                     QIII"
     gene            complement(19419..20777)
                     /locus_tag="ACGI59_001941"
     CDS             complement(19419..20777)
                     /locus_tag="ACGI59_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFG9739727.1"
                     /translation="MLRVALYIRVSTEEQALNGDSIRTQIEALEQYSKENDFNIVGKY
                     IDEGCSATNLKRPNLQRLLRDVEKDKVDLVLMTKIDRLSRGVKNYYKIMETFEKHKCD
                     WKTILENYDSSTAAGRLHINIMLSVAENEAAQTSERIKFVFQDKLRRKEVISGTIPIG
                     YKIENKHLVIDKEKKYIVKAIFDEYEKSGSVRTLIETINNLHGELYSYNKIKNILRNE
                     LYIGIYNKRGFYVEDYCEPIISKKQFKQIQRILEKNKKTTPNKNIHYHIFSGLLKCKE
                     CGYTLKGNSSNVGEKLYLSYRCSTFYLNKNCVHNVTHNEKHIENYLLTNLKPQLHKHM
                     VKLEAQNEKIRQNKKSNKKDEKKKIMKKLDKIKDLYLEDLIDKETYRKDYEKLQSQLD
                     NITEEQESQIIDTSHIKKFLDIDINEMYSDLSRVERRRFWLSIIDYIEIDNNKNITIN
                     FI"
     gene            complement(20842..21315)
                     /locus_tag="ACGI59_001942"
     CDS             complement(20842..21315)
                     /locus_tag="ACGI59_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFG9739728.1"
                     /translation="MNSYEKLLSEADDNNIIVREVSLISNSHGLYKNNRIALNKNTLN
                     NMSEKACVLAEELGHHYTSYGNILDLNKVENSKQEYKARLMAYNKLIGLKGIIDSFNA
                     GCKTITEMAEYLDVTEKFLNEALECYKSKYGFSATLDNYVIFFEPRFSIMNANFL"
     gene            complement(21333..21869)
                     /locus_tag="ACGI59_001943"
     CDS             complement(21333..21869)
                     /locus_tag="ACGI59_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFG9739729.1"
                     /translation="MKNTGHIIKILRIEKKLTQDELAKNLNSSFNLKLNKGMISKWES
                     NKAEPRFEYIKYISKYFDVSIDYLLGMTEYRNMYDNFDKKYDSKKLSLEVKEIEKNGE
                     KIKEKNKVLENKLLSSFSELNDIGQNEAIKRVDELTQIGKYVNKNHIDTIAAHNEHLH
                     EEGEIEKIYQDLDDMDNW"
     gene            22032..22241
                     /locus_tag="ACGI59_001944"
     CDS             22032..22241
                     /locus_tag="ACGI59_001944"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFG9739730.1"
                     /translation="MKSKRNPYLKFKIFLMERGIKQSELCKILGKSASAINQNINGTG
                     GDFSVEDLRIISQTYNISIDEFFIN"
     gene            22304..23194
                     /locus_tag="ACGI59_001945"
     CDS             22304..23194
                     /locus_tag="ACGI59_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HFG9739731.1"
                     /translation="MNEVVNAENINEIEVIGITEVDGMKFHDIEGGFGECKKAMLVKE
                     IAEIHNKELKHINELINKNRKRFQNDIDIIDLLGVGLNDTKIKKFGFTQQAINSYRGS
                     KNKGLLTGIYLLSERGYAKLLKILEDDKAWEQYEKIVDGYFNMRKSLKENNKFNSSKP
                     KDIKDESEIKYMNAQARLKNARAREAKIYLELADKVDIKEYKQIMYSKTTELLSGETL
                     IPLPKMEKKTYSATEIGKILGISANKVGGLANAYNLKTDEFGIKVWDKAKHSNKQVPN
                     FRYYENVMPILEKALSSFEN"
     gene            23251..23850
                     /locus_tag="ACGI59_001946"
     CDS             23251..23850
                     /locus_tag="ACGI59_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9739732.1"
                     /translation="MKENNRIVSVGTESEIDLTVKELNMIDDFKKIINGVNNEIANDY
                     SILEMDRDILEKIALSTALNYKKMNFEYSEIDEKLFNDFTFEFMLKFFGDIEEDELIK
                     YLVYIGEEEESYELNDRERIFYKIVDTLDTIADIKIADQNKIEKEVGMCIGEDNYITY
                     CIDENLIKFYIKDEEELVIDKDSPLLYMLDTLFYEVHEE"
     gene            23902..24090
                     /locus_tag="ACGI59_001947"
     CDS             23902..24090
                     /locus_tag="ACGI59_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9739733.1"
                     /translation="MGKKSEYTVTTVGEPNLDALGETLAKILTEKFGVLITHTYIENY
                     YVDKERGGDVDGAIESEF"
     gene            24065..24292
                     /locus_tag="ACGI59_001948"
     CDS             24065..24292
                     /locus_tag="ACGI59_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9739734.1"
                     /translation="MELLKVNFNLEKLKIGNVVSVSSKRFGFNINCIVVVATEEELNL
                     AYYSKDKGCIEYQALIPEDIRYGDYILQRLG"
BASE COUNT         9389 a         2862 c         4382 g         7894 t
ORIGIN      
        1 atactatatg ttgtaaatgt ttgtacattt aactatattt actttaactt gttaattaag
       61 ttttaatttt cattttcaag gatttttatt tttaaacttg atagagtatc atattaaaaa
      121 ttctttttat aaagttttat ttgcaaaaaa tacaaaaata taaacgttaa aaaaataacg
      181 ttatccttaa gtttttagat gatttcatta gtgatttgcg taaaaaataa agtgatatct
      241 agttaaatta agtatttgta aagctagata ttattagtag aaatgatgtt atattagata
      301 aaaaataaaa tgttaaaaaa ataacttgaa aataggaaaa gtaaatgcta taatgttaat
      361 aaattaacat aataaataaa aaactatctc aggaggttgt taaaatggct cataaagaac
      421 aagtaataat agaaaataaa aaagaaaatg aagttatgtc agttggttta gatactgtaa
      481 ataacgttga aagcttagtt aaaaaactag caaaaatcag agaagctcaa aaaatatttg
      541 ctacatatag tcaagagcag gtagataaaa tttttttagc agcatctttg gcagctaata
      601 agcaaagagt tcctctagct ataatggcac aaaaagaaac tggtatggga attgctgaag
      661 ataaggttat aaagaatcat tatgcctcag agtatatata taatgcatat aaagaaacta
      721 aaacttgtgg tgttatagag aaagacgaag catttggtat gactaaaatt gctgaaccaa
      781 taggtgttat tgcagcagtt gtacctacaa ctaatccaac ttctactgct atctttaaag
      841 cacttttagc tttaaaaact agaaatggaa taattttctc tccacatcca agagcaaaaa
      901 attctactat agaggctgca aaagttgttc ttgaggctgc tgttttagct ggagctcctg
      961 aaggtataat aggatggata gatgagccat ctttagaatt aactacaact gttatgaaag
     1021 aagtggattt gacccttgct acaggaggtc ctggaatggt taaatcagca tattcatctg
     1081 gaaaaccagc tattggagtt ggtgcaggta atactccagc aataattgat gatagtgcag
     1141 atataaaaac tgctgtaaac tcaattcttg tatctaagac ttttgacaat ggtatgatat
     1201 gtgcttctga acaatcggtt atagtattag aaaatatata caatgaagtc aaaaaagaat
     1261 ttaaagagcg tggtgcttat ttattagata aagatgaaac agaaaaaata agaaatataa
     1321 ttttagtaaa tggaggatta aattctaaaa tagttgggca aacagcttgt actatagcaa
     1381 aacttgcagg ctttgaagtc ccagtagata ctaaggtttt aataggtgaa gtagaatcag
     1441 ttgaaataga agaagctttt gctcatgaga aactttcacc agtacttgca atgtacaagg
     1501 caagtgattt tgatgatgca gttagaaaag cagaaaaatt agtagaagat ggtggatttg
     1561 ggcatacatc ttcattatat attgatgatg taaatcaaag agaaaaactt gataagttta
     1621 cttcagccat gaaaacttgt agaattttaa ttaatactcc ttcttctcaa ggtggtatag
     1681 gggacttata taattttaaa ctagctcctt ctcttacttt aggatgtggg tcttggggtg
     1741 gaaattcagt atctgaaaat gtaggagtta agcatttact taatattaaa acagtagctg
     1801 agaggagaga aaatatgctt tggtttagag caccagaaaa agtttatttc aaaaaaggtt
     1861 gtttaggagt agcattaaaa gaacttaaag atgtaatgaa taagaagaga gcttttattg
     1921 taacagatac attcctttac aataatggat atactaaagc agttactgat ttattagatg
     1981 aaatgaatat taagtacact acattctttg aagtagaacc agacccaact ttggaatgtg
     2041 ctaagatagg cgctaaagct atgagagaat ttaatccaga tgtaataata tctataggtg
     2101 gaggaagtgc aatggatgca ggaaagataa tgtggacatt gtatgagcat ccagatgtag
     2161 atttccaaga cctagctatg agatttatgg atataagaaa aagggtatat acattcccta
     2221 aaatgggtga aaaagctaat tttgtagcta taccaacatc agcaggtact ggttcagaag
     2281 taacaccatt tgctgttata actgaccaag atacaggagt aaaatatcca ttagcagatt
     2341 atgaactgat gccaaatatg gctatagttg attctgatat gatgatgaat atgccaaaaa
     2401 gcttaacatc tgcttctggt atagatgctt taactcatgc attagaagca tatgtatcta
     2461 tgcttgcaac cgattataca aatggattag ctttacaagc tattaagagt atatttgaat
     2521 atcttccacg tgcatatgat aatggtgcaa aagacccaga agctagagaa aaaatggcaa
     2581 atgcttctac aatggcaggt atggcttttg caaatgcatt tttgggtgtt tgtcactcta
     2641 tggctcataa attaggtgct ttccaccatg taccacatgg tgttgcaaat gctcttttga
     2701 taacagaagt tatgaaattt aattcttcag atgcaccaaa gaaaatggga gcattctctc
     2761 aatataaata tccagaagca ttaaagagat atgctgaaat agcttcattt ttaggattaa
     2821 aaggaaattc tgatgaagag aaattccaaa gtttattggt agctattgaa gatttaaaac
     2881 ttaaagttgg tattcctaaa tctataaaag agtttggcgt tgaagaatct aagtttatgg
     2941 attcaataga tgaaatggta attcaagcat ttgatgacca atgtacagga gcaaatccaa
     3001 gatatccact tatgaatgaa ataaaagata tgtatttaaa ttcatattat ggaagataag
     3061 tttgctataa aatagataaa ataaaaactc ttatacataa aacaaagttt tattgtataa
     3121 gagttttttt gtttgagaaa atggatagtt tttattcttt aatttaaaat tcaattaaat
     3181 agtgttatta taataataaa agaatgtttt tatggttatt atgttgattt tatgtgtaac
     3241 atttaagaat agttatatac aatataaatg atgaattggt ataaaaagga tatattatct
     3301 atatgaagta ttgactaatt aggggaggag tcgaatgaca gggagaaata aaagttttac
     3361 attaattaca attctttttt taacatcatt atttttgaca atggggtata tctatataga
     3421 agatacaaaa catcttctta tgtcagaggc aaaaatacat ataaaagaag ttgcagtaca
     3481 aggctctcaa ctagcacaaa gacaaattga aaaagattta gatgttttaa atattttttc
     3541 taactattat gcttcaaatc cagatatacc taatgaggaa aaaatgaaaa atcttttaga
     3601 tgaaatggaa aatcaaaaat tttacactat ggcaatagtt gatataaatg gtaatgctga
     3661 aaataccaaa ggtgataagt tttcagtgaa agatagagaa tttttcaaaa actcaattaa
     3721 aggtaagaag tatgtttcaa gtccatatgt tgatgaagta aataagtcta taaagaagat
     3781 tgctatttca gtaccattat taaataatga taaagtagtt ggagttttat attgtactta
     3841 caatatcaat actctgatga aacttattaa tatatctttt tatgaaaata atagtatatc
     3901 ttatgttgta aaaaataatg gtacaattat ccttcatcca caaggggata gcctatcaaa
     3961 aaatatttat aaattgttaa aacaggataa tgatattcaa gaagtaaata gattaaaaaa
     4021 agaattgcaa gaaaataaaa caggtgcaac tgtattaaat atgttggaag aaagaagata
     4081 tttaggatat gccactatgg ataatgggaa tagtgaaaat aattatataa aagactggaa
     4141 tttaattttt tcaattccag aaactgttgt atttagtaat tcaaaacaaa taatcaacag
     4201 agctgtatat gcagtgttat ctattgtatt aatatttatt gcaattattt tttatataat
     4261 ttatataaaa aaatctaatg aaaaagaaat attaagtttg gcttatgaag ataaagttac
     4321 atatatagga aatcaaaata aattttatag agaatgtagt aagtatcttt tagataaacc
     4381 atctttaaat tatattatag tatattttga tattaataac tttaagatga ttaatgatac
     4441 ttttggatat gaatttggtg acaatttatt aattacaata gcaaaagctt taaaagaaga
     4501 attaacagaa ggtgaggtat atgcacgact ttcaagtgat tattttgcaa ttttctgtga
     4561 ttataaaaat ggaagaaata aaattataag aaaacttgat aatatacgaa gtaatattga
     4621 aagtaatttg agtatagtct ttgaaatttc tctgtgtgtg ggtatttatt ttgttgaaga
     4681 aggtgaagta gatattcaaa aggctgtaaa taaagcaaat atggctaggt ctgttgctaa
     4741 aggtaaaaat ataaattatg ctatttataa tgaagatgtt aggaataagc ttagtgagga
     4801 atcaatgatt ttagatgata taaaaatagc attggtaaaa aatcaatttg aagtgtatta
     4861 tcaacctaag ttttcacttg taactggaga aatgataggg agtgaagcac taataagatg
     4921 gaatcatcca gaacatggtt ttataagtcc cgcagtattt attccaatag cagagaaaag
     4981 taagttgatt ttaaaaattg ggagatttgt atttgaaagg gtttgcaatg atttatatga
     5041 atggaaaaag caaggtaaaa agatagttcc agtttcagta aatttatcta gggtcgagtt
     5101 gtatcaacca gatattgtaa aatttatcaa taaaacaata aaaatgtata atttaagttc
     5161 agatttcatt gaaatagaga taacagaaac tgtggctatt aatgagttaa atattttaaa
     5221 gaatgtttta aatgagttga ggacgtatgg attttctatt tctatggatg actttggtac
     5281 aggatattct tctattagtt gtttaagaga tatgccaatt gatatattga agctagataa
     5341 gtcttttctt ggtggcattg aacatgatga gagaagtcgc aatatagcaa aatcaatagt
     5401 gtctctagca aaatcactag atttagttgt aattatagaa ggtgttgaga gtaaagaaca
     5461 agctgagtta atgaaacaat ttggatgtga tttagttcaa ggattttatt ttgcacgacc
     5521 aatgccagct aagaattttt tagatttact ttaaaacaaa atttgatttt tatataaagc
     5581 actattatga atgcttgatt ttaataaatc attgctaaat aattagtgtt tttttatttt
     5641 atcaatgtat tgtctaatat aaagcaaaaa ataattgtat tttataaaaa ttatattaaa
     5701 aataaattta ttttgctaat aaacatgtta ttaacaaaag aatagtacta taatgtatag
     5761 atgataaaaa ctttaataaa aatttagtgt ataggagttg tttaagtatg atagatataa
     5821 gtattgatga caaacttaag gaaagatggc ctaatgctaa gctgggttgc attcaagcaa
     5881 aagtagcagt aaataaaagc tcagaaaaat taattaatga aataaatgag ttttgtgata
     5941 ctataaatca aagtctaaaa gttgaagaca ttacgaaact agataaaatt aaagatgcaa
     6001 gaaatgctta taaagagctt ggaaaaaatc caagtagata tagaacatct tcagaagcat
     6061 tggttagaag aattattcaa gggaagggtc tttatacaat aaataatata gtagaaataa
     6121 acaatttaat atcaataaaa tcattatatc cagtaggtac atataatgta agcaaattac
     6181 attcaccagt atgttttact gtaggagatg aaggagaaca gtataagggt attgggaaag
     6241 aattaataaa tatagaaaat cttcctatat tatctgattc agtgggtaaa tttgggagtc
     6301 caactagtga ttctgaaaga gcaatgataa cagaagatgc aaatgaaata ttaatttgta
     6361 tattttcttt tagtcaagaa tctgatattg aagaatattt tggatatgct aaggaattac
     6421 ttaaagaata tgcagatgga aaagatatag aaactaaaat aattaaatag aatctatata
     6481 acaacgttat gttaagctaa aattcaatat aaaaataagt taagataaag aaactaacac
     6541 aaaattagtt tcttttttaa aatagtagtt tagtgttaca aaaatagata gatagtccca
     6601 cgattattaa aagtatggta atatattaat atagcagaga ttgtgtttaa gggattttga
     6661 aaggggggat ttataataat cattttatct aaaagataaa atggttttat agaaataact
     6721 atatatttaa aaatataaat aatgatacag taatttgggt atataaaata aggcatacat
     6781 ggttttgttt aaaaaccaat aaggtacata ggtaattaca atgtaaggaa ggatttaatt
     6841 atgattgatg gaaaagaggt aaaacaagaa agtggacaaa aaaatctaaa atttaagata
     6901 gctatagtag taggtgtttt gtttgtagct tatataggaa tggcaatctt ttttagaaat
     6961 catttttact ttggaacgac tattaatggc gtaaaggctt caggaaaaac agttgagcaa
     7021 gtaaatgatt tgttaacatc tagtactaat tcctatactt taaacttaga ggaaagaaac
     7081 aataagaaag aacaaataaa agcaaaagat attgatttaa aatttacacc agatgataaa
     7141 gttcaaaaga taaaagatag tcagagcgct attggatgga ttggtggaat atttaagtct
     7201 aaagagtata ataatatgat aactctttca tataataaag atttactaga aaaaaggttt
     7261 aatgctttat cttgttttga tagtaaaaat gttgtagaac ctaagagtgc ttatcctaag
     7321 tatgatagca ctaaaaacgc ttatgttata gtaccagagg tacttggaaa caaagtgaaa
     7381 aaagatgatt tttataagtt agtacaaaac tcaattaatt ctgggaatac ttctatttct
     7441 ctggatgaaa gtaaagttta tgaagcacca aacaacacaa aggattcaga aaagttgaaa
     7501 caagctatta agacattaga taagtatgtt ggtgtagtcg ttacatatga ttttagtgac
     7561 cgtaaggaaa ctttagatgg ttctgaaatt aataaatggt taaaagtaga ctcagaaaag
     7621 aattatgaca taacttttga taatgatgct atggtagaat atacaagagg tttaagtaaa
     7681 aaatacagta cttttggtga ctcaagacca tttaaaactg ccacaggtaa agatattaca
     7741 atttcaggtg gaatttatgg ttggttgata gataaaaaga aagaagctga agcattagtt
     7801 gatgttgtaa aagctggtaa aaatgttaca agagaaccta tttacataca aaaagctgta
     7861 tctcgtaata aagatgatat tggaaataca tatgtagaga ttagtctttc tggccaacat
     7921 atgtggttct ttaaaaatgg agaaatgctt gtatcaacag acgttgtaac aggtgattta
     7981 gctagaggat ttgctacacc atcaggtata tatcctctta attataaggc tagaaatgtt
     8041 tcattaactg gtcaaggtta cagttcacct gtaagttatt ggcttccgtt taatgggaac
     8101 ataggcattc atgatgctac ttggagaaat tcttttggtg gaagtatata taagtcttca
     8161 ggttcacatg gatgtgtaaa tacacctttt tctaaggcaa agaaaatata tgaaaatata
     8221 gaaccaggta caccaattat tttatactag taaatgtgtt gctaacagtt aagcgaagtc
     8281 atcttagttt atttaggatg gcttcctttt tatataaagc ttattgaagt ttacagaaag
     8341 actacttaaa aggagaaata cataatgaat gaaaaattaa ctaaaatttt agaagaagct
     8401 aagaatgatg atgaatttag aaaagaactt ataaaaacaa gagaacaacg tgatgttatg
     8461 gattcatttt gtaaggttgt tacatctaga ggttatgata tgacagtagg ggaacttttt
     8521 tctatgggag aagaatatac atccaatcta ctgaaaagtg taaatggagg agcagcatat
     8581 cctattgaag attggagtga ttggtacgag gattttttta taatgttgtc gaatatatag
     8641 agtaactaat gatattatct acttaagatt aatacattgc cttaaaataa aactattaga
     8701 actaggtgta agttttatca atctttaaaa atattgattt atttctaact agatataaaa
     8761 aaattagatt aaaaataagg tgacaaaaat cgatatagct tgatgattta taaaagttag
     8821 ataaaatagt gcgagatatt tctaaaaatt caaaaaaaac aataaacttc aatggttaga
     8881 ggttgaaaaa gaatataaaa aacttttata tgtaataaat taaattaaaa ttataaaaaa
     8941 aataaaataa aaatttaaaa tgtcgacata acgttaattt taaaacaaaa taacgctatt
     9001 gacaaactat atcgctttaa attataatta ttgaaaataa tggtatttaa aaaccatact
     9061 catgtcatag tctaaaagta agtcaaaaaa ggatgtaaat ctaggcaagt actattctaa
     9121 aatatgtttt attaagaaat aaacgttgaa aaatattaat tattatataa aaatgctgtt
     9181 tatactttaa caatgaggta gtaatctaat tagaataaaa aaatctaatt ggttatatac
     9241 atagtaaaaa ggtaagagag gtgatttgga tggcttttga aataataaaa agcattgttg
     9301 aggcagagca gacagcagac agtatcaaag taaaagctgt tactgatgca gagtctatca
     9361 gagctgatgc tgtaaacaaa tgtgaaagca tatttgctga tgtaaaaaaa caagcaaagc
     9421 ttatggaaga aactcttatt gagaaggcag tcaccgacag tagagcagag gttgataaaa
     9481 tcttagctaa tgctaaaagt gaatgtctga aaattgaaaa aactgctgaa gaaagaaaaa
     9541 gtaaggctat tgaagcggtt attggaaagg tagtgagata atatggcaat agttaatatg
     9601 aacaaaatat ctattgttgg cttagaagct caaaaaagcc agattttaaa gctacttatg
     9661 aaccgtgggt ttgttcagat tgatgatagt gcctttctta ctgaggagga ggaattaaaa
     9721 aattatcttg taaaagatag tgaagaatct gaagttataa tgctagagca gaaaatgtct
     9781 catgtaaacc aagcaattaa tattgtttct tcactagtga aacaaaaaaa atccctactt
     9841 gcaccaaaaa gagagtttaa caaaattgat agtgaaaaag aagggcaagt ttatgctgaa
     9901 gctctggagt taaacaattt gcacaaggag ataggtacaa taaggtctaa cataaactta
     9961 cttaacaaca ataaaagtat gttagaacca tgggtgaact ttgatattcc tcttgaaaat
    10021 atggaaacta agtatacaaa aactatactt ggaactgtgc ccataagcgc aaacattgat
    10081 gctttaagag taaaattaga agatgaaata ccagaaagtg ttattgggat tgtcaattct
    10141 gacaagttaa tgaactatgt ttatttaatt actttaaaag aaacttttga tgatgtttta
    10201 gaagttttaa aagaatttaa tttttcagtg gtttcattac caaatgaaga aggtacaccg
    10261 ttacaagcta tcaagaaata caatgaaatg ataattagac aggaaaacgt ttctaaggac
    10321 aaaacagagg aaattaaaag acatgcagat agtatacaca tgcttgaaaa cctatatgat
    10381 ttttacacta tagaaagaga ccaaaaaaag attatagaga gacttgtcaa aacaaaaaca
    10441 acattctgtc ttaatggttg gttaccaagc aaaagggcag atgaattaat taaagaaatt
    10501 acagaaaagt ttaattgttg tgttcagact gaagaaggaa gtaaagaaga aggcttccca
    10561 attctgcttg aaaacaataa attagtaaca ccttttgaaa gtgtaactaa catgtacagc
    10621 tatccaagta caaaagatat tgacccaaat acaattttaa ctttcttctt tgttgtattc
    10681 tttggaatga tgctaagtga cgccgcatat gggttaataa ttgcagtagt ctgtggattt
    10741 gttgtatata aattaaagat acaaaaaggt gaaggaaatt taattaaact tattggtatc
    10801 tgtggagttt caactactgt atggggtctt atattcgggg gtattttggg ggatttaatt
    10861 cctataaaag cattaattaa tcctttagag gatgttatga cacttatggg aatgtcgtta
    10921 ctgttcggta ttatccatat atatgtaggt ttgggaataa aagcatatac attaataaga
    10981 agtggaaagg taatagatgc aatctttgac ataggttttt ggtatttgtg tataacaggt
    11041 gtatgtctac ttataatacc ttttgttgca ggggatatag gtgtattttc agaagtagga
    11101 aaatatttag caattatagg tgctataggt cttgtactta ctcaaggaag aagctttaaa
    11161 ggtattcctg ttaagctttt taaaggattt tcaagtctct atggtataac aagttatttt
    11221 gctgatatat tatcttatac aagaattatg gcgctttgtt taacaacagg agttatagca
    11281 caagttatca accttttagg agctatagct ggtccgatac ttgcagttgt aattggtgtt
    11341 gtaggacaca caataaacct tcttataaat gcactaggtg catatgttca tacaagtagg
    11401 ttacagtatg tagaattttt caataagttt tatgaaggcg gaggagttcc ttttgttccg
    11461 tttaagtata aaacaaaata tacaagcatt aataaaaagg agatgtaaat atggaatttt
    11521 taaacgcgtt aggaaatggt caatttttgg caatatcagg agctgctata gcagcattat
    11581 ttgcaggtat gggttctgct aaaggtactt caatagcagg tcaagccgca gcaggtgttg
    11641 taacagaaga ccctagtaaa ttcggtcaac ttctagtttt acaacttctt cctggtacac
    11701 aaggtctgta tggatttatc atagctttcc ttattttatc aaaagcagga attattggtg
    11761 gagatgcaat accaacatca gctcaaggtt tacaacttct tatggcagga cttccaattg
    11821 gtttagttgg tttagtatca ggtatagcac aaggtaaagc agcagcagca ggagttggaa
    11881 tagttgcaaa aagacctgaa gaacttggta aagctataac atttgcagca atagttgaaa
    11941 catatgctct tttaggacta cttgtttctt tcttagctta caatggaatt gcaattggtt
    12001 aattataaat tataattcat aagtaaaaaa aatgaaaagg aggaagcact cctatgggta
    12061 atgaacaaaa aatgattgac agaattatag ctgatgccaa acaagaagca caagaaatat
    12121 tggataaagc taaaagtgaa gcagatttaa aagtaaattc agctaatgaa aaagctgaaa
    12181 aggaaatggc ttcctataca aagcttgcag aggcagaagc agaaaaagcg gcttctaagg
    12241 aaatatcagg agcttatatg gaggctaaaa agcaaatatt atcaaaaaaa caagagattt
    12301 tagaagaggt aattttagaa gctaaaaaca aacttttaaa tcttaaagat aatgaatatg
    12361 aagagattat actaaacatg attgaaaaat caaattgtac agatgattca gagatagtat
    12421 tatctaaaaa agataaaaaa acattaaaag atgtgctatc aaaaaaaggc attaaagtgt
    12481 ctgatgaaac tagagatatt actggaggtt tcatagttaa aaaaggtgac attgagtaca
    12541 attattcatt tgaagccata attgcagtag agcatgaata tattgaacaa attgcagcag
    12601 aaattttgtt taattaagga ggggttaaaa aatggcagaa gaaaaaactt atccctatgc
    12661 cgttgcacga ataagagtgc ttgaaaaaca gcttcttaac agacaaatgt ttattcaaat
    12721 ggcagaagcc aaaagccctg aagactcttt aagaattata gctgaggcag gttatgctga
    12781 tacatcaaat aataatatac ataattttga gaatattttg actcaagaat tatcaaagac
    12841 atatgaaatt cttaaaagtc tagctcctga agaaaaattt gttgatgtat ttctttataa
    12901 aaatgactat cacaatttaa aagtgctgat taaagaagaa atttctggtg tagatggtga
    12961 gaaatattta attgatggag gtacaattcc tcttattaag cttagagaat ctttagcaaa
    13021 tcgtagtttt agtgatttac ctaagataat gtcatcagct atattagagg catttgatgt
    13081 ttataataag acacaaaatg gccagatggt tgatattgtt ctagataaag caacctttac
    13141 cagtatgaaa gaaactgcta aagaaagcca aaacaaattt gtaatagatt atgttatgaa
    13201 agtttgtgat ttaacaaatc taaagagttt tataagagtc aaaaatatga agaaagattt
    13261 tgatatgttt atgaatgtgt ttgtttcagg tggctcactt gataaggaaa aattcttaga
    13321 agctttttcc tcagatactc cagcatcatg ttttaaatct acttcttact ctgatgtctg
    13381 taaaaatggc atggatagtg gttttactgt ctttgaaaaa ctttgtgatg actatcttat
    13441 ggaatatgtt agaggagcaa aatttaagcc tcttacatta gaaccactaa ttgcatattt
    13501 atatgctaaa gaatcagaga taaaaacaat tagaattatt ttgactagta agcttaataa
    13561 tatagacgca gatactataa aagaaaggct aagggatgct tatgtataag gtaggagtag
    13621 ttggagataa agatagcatc atgggatttt tagctcttgg aatagatatc ttcccagcct
    13681 atgatagtga cgaaattaaa aaaagtatac ataagttggt tgaagatgaa tatgcgataa
    13741 tttatattac agaacaggca tcattgcttg caaaagaatc aatagcaaaa tacaaagatt
    13801 accaacttcc tgccattata gttattccag gtattggagg aagtatggga ttaggtatga
    13861 atgaagtaag agaatctagt aaacgtgcta taggtgcaga tatattattt aaagaataga
    13921 tgaaagttga ataaaagaca atctcaaatt ttattggtgc taaatatatg tgctaataaa
    13981 atataggagg gaatatttaa attttgataa agtatatgat tgcaggtgag agaatatgaa
    14041 aacaggcaca attgtaaagg tgtcaggacc gcttgttgta gcggagggca tgagagatgc
    14101 gaatatgttt gacgtcgtaa gagtttcaga taaacatctt ataggcgaga ttatagaaat
    14161 gcatggagat aaagcttcta tacaggttta tgaggaaact tccggtctag gaccagggga
    14221 agaagtagtt tctcttggaa tgcctatgag tgttgaactt ggaccaggtc ttatttcaac
    14281 tatttatgat ggtattcaac gtccacttga aaaaatgtat gatatttcag gtactaatat
    14341 aactaaaggt gttgaagtat cttcacttga tagaacaact aagtgggaat ttaaacctaa
    14401 aaaatctgtt ggtgataagg ttgtagcagg agatataatt ggtggcgttc aagaaacagc
    14461 agtagtagaa tgtaaaatta tggttcctta tggagttaag ggtactataa aagaaatata
    14521 ttctggtgaa tttactgttg aagatacagt ttgtgttatt acagatgaaa aaggtaatga
    14581 tatacctgta actatgatgc aaaaatggcc agttagaaaa gaaagaccat atagagaaaa
    14641 gaaaacacca gattcacttc ttgttacagg tcaaagagtt atagatacat ttttcccaat
    14701 cacaaaaggt ggggtagcag ctataccggg accttttgga agtggaaaaa ctgttacaca
    14761 gcatcagctt gctaaatggg cagatgcaga tatagttgta tatataggct gtggcgagcg
    14821 tggaaatgaa atgacagatg ttcttcttga atttcctgaa ttaaaagacc caagaacagg
    14881 cgagtcactt atgcaaagaa ctgtgcttat agcaaataca tcagatatgc cggttgctgc
    14941 acgtgaagct tctatataca ctggtattac aatagctgaa tattttagag atatgggata
    15001 tagtgttgca cttatggcag actctacatc aagatgggct gaggctctta gagagatgag
    15061 tggtcgttta gaggagatgc ctggtgaaga aggttatcct gcatacttag gttcacgtct
    15121 tgctcaattc tatgagagag caggaaaggt aaattgtcta ggtatggatg aaagagaagg
    15181 aacacttaca gcaattggtg cagtttctcc tcctggtggt gatacatcag aacctgtcag
    15241 ccaagcaact cttcgtatag taaaagtatt ctggggtctt gatgcatctt tagcatataa
    15301 acgtcatttc ccagcaatta actggttgac tagttattca ctctatcagg aaaaagttga
    15361 tgtatgggca gataaaaatg taaatgataa tttctcagct caaagagctc aagctatgaa
    15421 acttttacaa gttgaatctg aacttcaaga aatagttcaa ttggttgggg ttgattcact
    15481 ttcagatgga gatagattga tactagaggt tactcgttct ataagagaag acttccttca
    15541 tcaaaactct ttccatgaag ttgatactta tacatcactt cataaacaat atagaatgat
    15601 gggacttata ttaaaattct ataagtcagc acaagatgct atcaagcaaa atgttactat
    15661 aaacgatatt atcaagcttt cagttcttga aaagattggt cgtgctaagt atgtagaaga
    15721 aggcgatata gatgcagctt acgattcaat tgaagatgaa atagataacg agcttgcaga
    15781 ccttattaaa aagagggggg ctgaagaact atgataaagg aatataagtc aatacaagag
    15841 gtagccggtc ctcttatgtt aataaacggc gtagaaggtg taaaatttga tgaacttgga
    15901 gaaattgaac tttcaaatgg agaaattcgc cgttgcaaag ttctagaggt aaatggagat
    15961 actgcacttg ttcagctttt cgaaagctca acaggtataa accttgctga aagtaaagtt
    16021 cgttttttag gaaagagtct tgactttgga gtttcaccag atatattagg tcgtgttttt
    16081 agtggaatgg gtagacctat tgatggaggt ccagaaatta taccagataa aagacttgat
    16141 ataaatggag ctccaataaa tccagcagca cgtgattatc cggccgagtt tatacaaact
    16201 ggagtctctg ctatagatgg acttaataca ttggttagag ggcaaaaact tcctatattc
    16261 tcagggtctg gtttgccaca tgccaatctt gcagttcaga ttgcaagaca ggcaaaagta
    16321 cgtggaactg attctaagtt cgccgttgta tttgctgcag ttggtataac ttttgaagat
    16381 gctgaatttt ttataagcga ttttaaagca actggtgcaa tcgaccgttc tgtagtgttt
    16441 gtaaaccttg caaatgaccc agctgtagag cgtgtatcta cacctagaat ggcacttaca
    16501 gcagcagaat accttgcttt cgagcaagat atgcatgtac ttgttataat aacggatatt
    16561 acaaactatg cagaggcgtt gcgtgaagtt tcagcagcta aaaaagaagt tccaggacgt
    16621 cgtggatatc caggatatct ttatactgac cttgctacta tgtatgaaag agcaggaaaa
    16681 atgaaagggc atgaagggtc tataactatg attccaatac ttacaatgcc tgaggatgat
    16741 aagacacatc caattcctga ccttacagga tacattacag aagggcaaat tatacttagc
    16801 cgtgaacttt ataaaaaaga tatacagcct cctatagatg ttttaccatc actttctcgt
    16861 ctaaaagata aaggtattgg taaaggtaag actcgtgaag accatgcaga tacaatgaac
    16921 cagctttttg cagcttattc aagaggtaag gatgcaaaag aacttatggc tatacttggt
    16981 gaatctgcac tttctgagat tgatttaatg tatgctaagt ttgcagatga atttgagaaa
    17041 gaatatgttt cacaaggatt tagaactgat agaactattg agcagacatt agaaatcgga
    17101 tggaagcttc ttagtatgtt acccaaaagt gaacttaaac gtatccgtga tgaatatttt
    17161 gaaaaatata tgcctaagga gtgatatctg tggctagatt aaatataaat ccaacacgta
    17221 tggaaatgac tagacttaag aaactcctta aaactgctac aagaggtcat aagcttctta
    17281 aagataaact tgatgaactt atgaaacaat ttcttgagat tgtaagagaa aataagcgat
    17341 tgcgtgaaga agcagaaaat gctcttgata ctgcttataa aaactttata atcgcaagag
    17401 cagttatgag tcaggagtat ctgggttcag cacttatgat gccaaagcaa tcagtttcag
    17461 tagatgtttc tacgagaaat ataatgagtg ttgatgttcc tgtgtttgac tttaaaactg
    17521 aaaacaatca aagtgatatt tatccttatg gtcttgcttt tacatcaggt gaattagatt
    17581 ctgctatgga agctttctca gatgctatgc aaccactttt aagacttgca gaaagtgaaa
    17641 aaagtgcaca gcttcttgca caagagatag aaaagacacg tagacgtgtt aatgctcttg
    17701 aaaatgtaat gataccaaat tacattgaga ctattaagta tatagctatg aagcttgaag
    17761 aaaatgagag agcaagtact actagactta tgaaggttaa ggatatggtg cttaaaaaag
    17821 cacttgaaga aaagaaaaaa aatgatttgg ttgtataatt aaatacttat aaaaataagt
    17881 ctaacattct actttataga ggttagactt attttttatg tttaaattaa aatgtagata
    17941 ctataaaaat taatatattg tttaaaaaca gactgtattt aactaaaaat aatatattta
    18001 acaaaaattt ctaaaaatta acgaactgtt aatttacact taactttata ttaacaaatt
    18061 aggtgtatta tataaacata agcaaaaaca ataaaaaaca aataaaaaaa ggaaaaaaca
    18121 caaaggggga agatatgaaa gaatttatat ttacttgtct gtgcattgta gtgattacat
    18181 ttatgacaag ttgcatatca acaacaccac aaactactca aggaaaagga aataataaac
    18241 ttacaatata tgcagcacta gaggaagagc agatagctta ttatatggaa ggttttagag
    18301 aagagtatcc caatatagat gtagagatag ttatggattc tcatggtgta atagcttcaa
    18361 aactattagc agaaaaagat aatccacaag cagatattat atggggatta tcagcaatga
    18421 acatgataga gctaaaaaaa gatggtgcac ttgaagcata taagcctaaa aattatgata
    18481 aagttgataa aaaatttaaa gatactgaag atgatgtatc ttgggtagga atgagtgtcc
    18541 cagagacagc aatagtagtt aattataagg aacttaagaa actaggtata gatattccaa
    18601 aaagttatga agatttaata aaaccagaat ataaaggact tataacaatg ccaaatccag
    18661 catcatctgg aacggggtat cttacagtat cagggcttat tcaaatgatg ggtgaaaaag
    18721 atgcttacaa ttacatggat aaattacata aaaatatagc cacatataca cactcgggtt
    18781 caaaacctgc gaagttagct ggctctggag agtatccaat aggtattaca cttggatata
    18841 gagctacaca acaattatct agtggagaac ctatagaggt tgtatttcca aaggaaggtt
    18901 caggctggga cttagaagca aatgccttag ttaaaaaaga taatataaaa aaagaagcaa
    18961 agctattttt agattgggca ataagtgatt cagctatgga tagatatgca catgaagtag
    19021 cagttacttc cataaagaca gataatccag ttccaaaagg ttattctaaa caaccatttg
    19081 aacagcttat aaaagaagtt gatttaaact ctgctgccaa aaacagaagt acagtactta
    19141 aaacttggga atcaaaatac ggttcaaaat ctgaaagtaa gtaaagttta aattaataat
    19201 ttagatgaaa gaattttaga agaaaaagta ggatataaaa tttaaaataa aaaaataact
    19261 atataaggag agataagata tgagttactt aaaaataaat aatgtgttta aaagttatga
    19321 ccaaaaaagg gttttaaata atattagttt ggatattgaa gaaggagaat ttctatgttt
    19381 actcggacct agtggatact atatgttagt ccaaataatt atataaaatt aatagtgatg
    19441 tttttgttat tatctatttc tatataatct attatagata accaaaaacg tctgcgttca
    19501 accctgctta aatcactata catctcatta atatcaatat ctaagaattt ttttatatgt
    19561 gatgtatcaa taatttgact ctcctgttct tcagttatat tatccagttg agattgtaat
    19621 ttttcataat cttttctata agtctcttta tcaatcaaat cctctaaata taaatctttg
    19681 attttatcta attttttcat aattttcttc ttttcatctt ttttgttaga ttttttattt
    19741 tgtctgattt tttcattttg tgcttctaat ttcaccatat gtttatgtaa ttgtggtttt
    19801 aagttagtca aaaggtagtt ttctatatgt ttttcattat gagttacatt gtgtacacag
    19861 tttttattta gatagaatgt agaacatcta taagataaat aaagtttttc tccaacatta
    19921 ctagaattac cctttaatgt ataaccacac tccttacatt ttaaaagacc agaaaaaata
    19981 tgataatgta tgtttttatt aggagtagtt tttttatttt tttctaatat tctttggatt
    20041 tgtttaaatt gtttttttga tataataggt tcacaataat cttcaacata aaatcctcgt
    20101 ttattataga ttcctatata aagttcattt cttagtatat tctttatttt attataactg
    20161 tataattcgc catgaagatt attaattgtt tctattaaag tcctaacaga acctgatttc
    20221 tcgtattcgt caaatatagc tttaactata tatttttttt ctttatcaat aactagatgc
    20281 ttattttcta ttttgtaacc tataggaatt gtaccactta taacttcttt acgtctcaat
    20341 ttatcctgaa acacaaattt tattctctca gatgtttgag ctgcttcatt ttctgctaca
    20401 gataacataa tgtttatatg taatctacca gcagcagtac tagaatcata gttttctaaa
    20461 atggttttcc aatcacattt atgtttttca aaagtctcca ttattttata atagttttta
    20521 acgccacgac ttagtctatc aattttagtc attaaaacta ggtctacttt atctttttcc
    20581 acatctctta ataatcgttg taagttaggt ctttttaaat tagttgcaga gcaaccttca
    20641 tcaatgtatt taccaactat gttaaaatca ttctctttag agtactgttc taaagcctct
    20701 atttgtgttc ttatgctatc tccatttaaa gcttgttctt ctgtactaac acgtatataa
    20761 agtgctactc ttaacatttt acattccccc ttattgtata tttaagagga gctgaacggc
    20821 tcttaaatat actatgaatt gttacaaaaa atttgcattc ataatactaa atcttggctc
    20881 aaaaaatatc acataattat ctagtgtagc tgaaaaacca tatttacttt tataacattc
    20941 taaagcttca tttagaaact tttcagttac atcaaggtat tctgccattt cggttatagt
    21001 cttacagcca gcattaaaac tatctattat acctttaaga ccaattaact tgttgtaagc
    21061 catcaatcta gctttatatt cttgtttgct attttcaact ttatttaggt ctaatatatt
    21121 tccatatgaa gtataatgat gccctagttc ttctgctaac acacaagctt tttcactcat
    21181 attattaagt gtatttttat ttaaagctat tctattattt ttatataatc catgagaatt
    21241 tgaaattaaa gaaacttctc taacaataat attattatca tctgcttcag agagtaactt
    21301 ttcataacta ttcataagtt cacctaccta ttttaccaat tatccatatc atctaaatct
    21361 tgatatattt tttcaatttc tccttcttca tgtaaatgtt cattgtgtgc tgctattgta
    21421 tctatgtgat ttttatttac atatttacct atttgagtaa gttcatctac tctttttatt
    21481 gcttcatttt gtccaatatc gtttaactca ctaaagctag ataaaagctt attttctaat
    21541 actttatttt tttctttaat cttttctccg tttttttcaa tctctttaac ttctaaactt
    21601 aattttttag aatcatattt cttatcaaag ttatcataca tatttctata ctctgtcatt
    21661 cctaataaat agtctattga aacatcaaaa tactttgata tatattttat atattcaaat
    21721 ctgggttctg ctttgtttga ttcccatttt gaaatcatac ctttatttaa ttttaaatta
    21781 aaactagaat ttagattctt tgcgagttca tcttgcgtta attttttttc aattcttaaa
    21841 atctttataa tatgacctgt atttttcatg gattacacct ctttataatt tagatatact
    21901 taaattatat aacatgaggt ttcattgtca atgatttaat ttaaaaaaaa taaagaaagt
    21961 tattgacaat gaaacttgca ataatatata atttaggtat caaatatgaa acaaagaagg
    22021 gggtgaaaaa cttgaaaagt aaacgtaacc catatctcaa atttaagatt tttttaatgg
    22081 aaagaggaat taaacaatct gagttatgta aaatacttgg aaagtcagca tctgctataa
    22141 atcaaaatat aaatggaact ggaggtgatt ttagtgttga agatttaaga attatatcac
    22201 agacatataa tataagtata gatgaatttt ttattaatta aaaagtttct tatatgaaac
    22261 aagagctgtt taaaaaatac aaaaaactag ggaggaagaa attatgaacg aagttgtaaa
    22321 tgcagaaaat ataaatgaaa tagaagtgat aggaattaca gaagttgatg gtatgaaatt
    22381 ccatgatatc gaaggaggtt ttggagaatg caaaaaagca atgttagtta aagaaatagc
    22441 tgaaatacat aataaagagt taaagcatat aaatgagctt ataaataaga atagaaaaag
    22501 atttcaaaat gacatagata taattgattt gttaggtgtc ggtttgaacg acaccaaaat
    22561 aaagaagttc ggatttactc aacaagcaat taattcttat agaggttcaa aaaataaggg
    22621 tctattgaca gggatatatt tgttatctga aaggggttat gcaaagttac ttaaaatact
    22681 agaagatgat aaagcatggg agcaatatga gaaaatagtt gatggatatt ttaatatgag
    22741 gaagagttta aaagaaaata ataaatttaa tagtagcaaa cctaaagata ttaaggatga
    22801 atcagaaata aaatatatga atgctcaagc aagattaaaa aatgcaaggg caagagaagc
    22861 aaaaatatat ttagagttag ctgacaaagt agatataaaa gaatataaac agattatgta
    22921 ttcaaaaact actgaattac tttctggaga aacattaata ccacttccta aaatggagaa
    22981 aaaaacttat tctgctacag agataggtaa gatattaggg atttcagcca ataaggttgg
    23041 aggattagca aatgcttata atttaaaaac agatgagttc ggaattaaag tttgggataa
    23101 ggctaaacat tcaaataaac aagtgcctaa ttttagatat tatgagaatg taatgccaat
    23161 tctagaaaaa gctttaagta gttttgaaaa ttaaattcag aagttgctaa gtacaacttt
    23221 agaaacttaa atttaaggag gaataagaaa atgaaagaaa ataacagaat agtaagtgtt
    23281 ggaactgaaa gtgagataga tttaacagtt aaggaattga atatgataga tgatttcaag
    23341 aaaattataa atggtgttaa caatgaaata gctaatgatt attctatttt agaaatggat
    23401 agagatattt tagagaaaat tgcattaagt acagctttga actataaaaa aatgaatttt
    23461 gagtattcag aaatagatga aaaactattt aatgatttta cttttgagtt tatgctaaag
    23521 ttttttggtg atatagaaga ggatgaatta atcaaatatc tcgtttatat aggagaggaa
    23581 gaggaaagtt atgaattgaa tgatagagaa aggatatttt ataaaatagt agatactctt
    23641 gatacaatag cagatataaa aatagcagac caaaataaga tagaaaaaga agtaggtatg
    23701 tgtatcggtg aagataatta tataacttat tgcatagatg aaaatttaat aaaattctac
    23761 attaaagatg aagaagaatt agttatagat aaggatagtc ctttgttata tatgctagac
    23821 acgttatttt atgaagtaca tgaagagtaa aaattgcaat gattaaatac ggaatatttt
    23881 gaaaatagag gggtgattta aatgggtaaa aaaagtgaat acacagtaac aacagttggt
    23941 gaaccaaatt tagatgcatt aggcgaaact ttagctaaaa ttcttacaga gaaatttggg
    24001 gtactaataa ctcatacata cattgagaac tattatgttg ataaagagag agggggtgat
    24061 gtagatggag ctattgaaag tgaattttaa tctagaaaaa ttaaaaatag gaaatgtagt
    24121 gagtgtaagt tctaagaggt ttgggttcaa tattaattgt attgtagtag tagcaaccga
    24181 ggaggaacta aatttagctt attatagcaa agataaggga tgtattgagt atcaagcatt
    24241 gataccagag gatattagat atggtgatta cattcttcaa agactaggtt aggaggaaat
    24301 aaaatagcag ttttaataat ggtaggttta tttgcaatat gtttagtagg attatttcaa
    24361 taaaaaagtg ctggtcaaag taaccaacac acaaaataaa aaaagttaat aaaattataa
    24421 cataaaaagg agagatttaa aatgcttgaa gttaatataa aaatcaaagg tgaagcaagt
    24481 gaaataattg actcaattag agctatattt aatagctttg gtgaaga
//
LOCUS       DAYAPA010000052        24491 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.052, whole genome shotgun sequence.
ACCESSION   DAYAPA010000052 DAYAPA010000000
VERSION     DAYAPA010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24491
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.052"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            116..1504
                     /locus_tag="ACGI59_001949"
     CDS             116..1504
                     /locus_tag="ACGI59_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LVIVD repeat-containing protein"
                     /protein_id="HFG9739735.1"
                     /translation="MEDKFYAKGNGNNGYIKNLEVCSFNNLDGTCGMFQMALYKRDEK
                     YYLYGCCFGGNKKNGVMISDITDPYNPQFIKHFQMLDPKEYPTTTTPKIQIADDLMIV
                     AMSCGSGPGALVDQAKLANIKCEAGIRIYSLKEDPLNPKFLGYWDCGLKHVMGVHRFM
                     YNGGRYVHLSSDCVGFEGLIYRVIDIINPTNPVEIGKWWRPDQYANGYPNRTFDAGAP
                     HCPEFMDKGWLHGPPFVRDGKAYCGYGGAGLVVLDVEDLTRPRCLGELPFMPAFSSRL
                     AGARTHTALPLPGRDLVVVQNEGERFQFFKPDNITDVQAMNNIHMVDVSDPTKPTLIA
                     QFPYPEVPKDFPYPNFNVAGLGKPGPFGPHNLHEPMDNKPWLEQRGDRVYCCYFHAGL
                     RVYDVSDPYYIKELAYFIPPNPNKTPEESYFPGFPGPRLAVTEDLIVDDRGYIIIDAL
                     DDGFYILKMKED"
     gene            1532..2485
                     /locus_tag="ACGI59_001950"
     CDS             1532..2485
                     /locus_tag="ACGI59_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HFG9739736.1"
                     /translation="MKILGISFGTKNGNNDTMCKVALKGAKEAGAEVEFIQMSSINIK
                     HCTGCCACVKTLLSGKGSMCVLKDDFEWLLDKMKDADGIVVSDPIFEEGASGLFHTIM
                     DRFGPRADRGNNIIGTKVAEAIGGKIPDSRMLNDKVISFMGIGGSDWGTRVQCEHAML
                     ALIPMWKVIDNAWFPWAKELVMDDTRLVQVHQIGLNIVEAAKDFEKASYQGEEGVCPH
                     CHNRLFYLEPGTKKAICALCGIVGELDTISGKTIFSFPEEQLGHAHDTLPGKFIHGDD
                     IKKMEGHYAEVRKTQEFKERKTSYNFIEPLTKEKQAGVSLG"
     gene            2545..2769
                     /locus_tag="ACGI59_001951"
     CDS             2545..2769
                     /locus_tag="ACGI59_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9739737.1"
                     /translation="MNEKSSFKQIEIILAVIFLVLTYYKVRLTPAFIVIFIPLYLSYF
                     AVSMIVNQIAIKLLEIRQAREATNYNIGNG"
     gene            2762..3274
                     /locus_tag="ACGI59_001952"
     CDS             2762..3274
                     /locus_tag="ACGI59_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFG9739738.1"
                     /translation="MDRKKKEILTLSMGIALLPPLWAVLAPYIGIKTGAVALICAGLY
                     VTNGNKQKDGLKIMFGFWCGDLWAVLAILIMGYMNFNQNLELFLTLSILGFFAVVIAS
                     LFEKIIFLPSWLCGWAIGLTIMTGENIINLQDICIQIAVAMAVGVWYVGAGVDLFTIY
                     ILSKDRKKGN"
     gene            3306..4160
                     /locus_tag="ACGI59_001953"
     CDS             3306..4160
                     /locus_tag="ACGI59_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase"
                     /protein_id="HFG9739739.1"
                     /translation="MAMGFEFKIMRSLIYVGLAKEEYRPKLMDWLYRHHIPDSISTFG
                     PYCTKYAFYQAYPTPNEGERFGARKMQLTEHYWLVDEHMPEMANRIMTEYMPMDVLRW
                     QGCIPDVENKRVHENAESGDAGRAVGGDNGCPPFIFAFVPINWEEDFRGKGRTVQDGP
                     NYRWQFMIKYPDGISKEEGEKWFYDEVVPYFTNCCYVNRFVSSKIMINYGATAFDRVS
                     ELWFEGEEEWYKAVVEETKSFIKKPEWAQEEEFPYLKPQFNIASVFLGDIATMDAYSQ
                     YRGYIPMR"
     gene            4694..5488
                     /locus_tag="ACGI59_001954"
     CDS             4694..5488
                     /locus_tag="ACGI59_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFG9739740.1"
                     /translation="MYTIGQVSKFLGISRDTLKFYEDKGLVNPKKNDENGYRIYNQVD
                     IYDIATINFYREIDIEIKKIQEIRKSKSINNLELLLEEKEQIILKEIEYKKLLLKKVQ
                     LVKEDCDKIKTYFGKYTIKEMKPLEVKGKIKHVTNYEEYEALKENTNNLKKAVTLTDL
                     RRVINFNEEGIIEEKFIIVREVDDYNKIIEGEVLSHPNCIYTIIEDGRWSTGGENIDN
                     KVEDRLREIAIENECELLGIVYVNILLTTYEEGIERIFLEIYAPIK"
     gene            5592..6398
                     /locus_tag="ACGI59_001955"
     CDS             5592..6398
                     /locus_tag="ACGI59_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFG9739741.1"
                     /translation="MRTEKSLKRPIASFIILTNIIFLPLFLLVVVTIMLKLPTVISDI
                     ALCISAWSSTFAFMILFKKIYPGKKFLVHVKDRFRNKLKFSTVSIIISIQVLIAVVVI
                     FILASKNHGIMPNFTVSSWGAFIYLFLKNLFAGSLGEELGWRGFVQNHLQKRHSPLKA
                     SIIVGFWWGMWHLPIWFTTGFKGLDLIKYIIFFMIAIISISIIMTTFYNLNKTLIIPI
                     VIHQLFNFLIGIINGDLIELINYYAMLYLIFAIALIVINPGNVLYKNIRE"
     gene            6460..6981
                     /locus_tag="ACGI59_001956"
     CDS             6460..6981
                     /locus_tag="ACGI59_001956"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFG9739742.1"
                     /translation="MVLLIVDAQKLITNERLYKFNEFVVNVENLIDTARKNNIEVIYV
                     RHDDGVENELTKGKNGFEIYEKFKPCNGEKIFDKKVNSAFKETGLLEYLTNNGEKDII
                     IAGLQTDYCIDATIKCGFEHGFNIIVPAYSNTTVDNFFMSAEQSYKYYNEFIWNERYA
                     KCISLEETLRKMK"
     gene            7396..9483
                     /locus_tag="ACGI59_001957"
     CDS             7396..9483
                     /locus_tag="ACGI59_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFG9739743.1"
                     /translation="MVVDKEIVSILQILLNSTLITTNGLQEESNSSKRQITYRINKIN
                     DMLKVKNVPLICLRADKDIIVRKETKKAIKEILEKNYSKNTYYFSKTERLLYMYLMLF
                     INMDDISINHFINSIKVSRSTVNLDFKDLIPELEKKNIKVKNNRINGYYIVGNEMEIR
                     RVLIKNIIETLSNEKSSRVFDIFIKEYNLDSFEEAKKIILKLIKKYEITFVEDRLVEF
                     IYIFIFLKARMYSTRIVTQENVDIPNISVMSSMKEYKFAKELLEVYGSEDKIKPHNIM
                     YISAWILGISVGNYEEDTEDKDVISKIVNKMMTKFGYLSGVYYISQEKIFKQLYSHFR
                     PAYYRLLFKLPIYNPICEKVKEEYKLVYRIVSNAMKEFCGLFGEEIQEEELAYLTMHF
                     ATLFSNKKEFDGFRKRIALIVCSNGVGSSAILHAELTNLFPDLHFIPMSESVGLENIS
                     EPVDIVFTTNYNAEGLKTDVPVIKVSPVMTTKEKYKITREVYIQLGDIFLRQPKIDEV
                     MDIIKKYANVTSESILSSELLAYFTQIENFTSKEGEGPMLSEITNETLVKIKVKAKNW
                     EEAIRQSASVLVENNKVTEGYVDAMINSAKASGPYIVITKHVALPHARPEAGSKEIAI
                     GIATLDSPIEFGNNENDPVKYVFCLSAIDNNSHLRAMSELVELLEEDEFFKVLDSAKE
                     AKEIIDFIKTHEL"
     gene            9498..9785
                     /locus_tag="ACGI59_001958"
     CDS             9498..9785
                     /locus_tag="ACGI59_001958"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFG9739744.1"
                     /translation="MYKILIACRAGVGSSLMLKIKTQQIIKENNFPIEVEHGSLDSLN
                     GFNGDAVETLIDVAEELKNKNLKFDIIGIHNIVDRNEIKTALEKFLASKEG"
     gene            9812..11083
                     /locus_tag="ACGI59_001959"
     CDS             9812..11083
                     /locus_tag="ACGI59_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFG9739745.1"
                     /translation="MEFIISLLSTPAVLLGVVACIGLILQKKSAVEVFTGSSKTLIGF
                     LIFGIGASAMTGALQNFNKLFQHGFGITGVIASPEAATALAQGTYGFAVSCTLILGFI
                     LNLVFARITKFKNIFFTTGHSLFFSCVLVLIMKAHGFDNTITILIGGTILGFMSAALP
                     QFCQPFMRELTGGDEQAIGHFNMIGYGLSGYIGRLFSKHKDDTTETIEFPKWLSIFRD
                     FLMGLSIVMLILFYIATLKAGQAFTQEIAGSTHWLVFPLIEAFTFVAGMSILMSGVRM
                     FLAEITAAFVIISEKYIPGSRPALDVPTVFPYAPNAVIIGFISAYAAGLLAIAIMASF
                     PSIFPVVIIPAAHICFFSGGTAAIFGNTSGGWRGAIAGSFVVGLLLAFLPVILYPVYG
                     TLGIEGATFPNIDYNVVGSILHNILQFIKPI"
     gene            11141..11740
                     /locus_tag="ACGI59_001960"
     CDS             11141..11740
                     /locus_tag="ACGI59_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFG9739746.1"
                     /translation="MENTLSEFLDNISNELSEFIASIDETSINKACELILEAEKNHGR
                     VHVTGIGKPGHVSGYISSLLSSTGTSAYILHGTEAVHGSSGQVVEGDVVIAISNSGET
                     QELKATLKTLKVNGAKIIGVSGNESSFLKNISDIFLFAGVKQEGDCLNKAPRASILAE
                     TIILQSLSVVLQYAKGLNTQEYLKWHPAGSLGKSIREGI"
     gene            11745..12650
                     /gene="dapA"
                     /locus_tag="ACGI59_001961"
     CDS             11745..12650
                     /gene="dapA"
                     /locus_tag="ACGI59_001961"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFG9739747.1"
                     /translation="MKYKGIICAMITPFDENQNINSQATCQLIDYLIEKGIYGLFILG
                     TNGECHVLTDDEKVEFAKIVINHTNNRVPVFVGTGGNSTREVVDLSKRMEAIGASALS
                     IITPYFVTPTQQELILHYKTIANSVNLPIIMYNMPGKTGINIEPESVRELSKIKNIVG
                     IKDSSGKLDNMKAYIEATKNENFSVFSGSDSLILDSLKAGSQGAVAATTNFLTEIGIS
                     IYENFMKGDLEEAQKSQSSIEELRRILKLGTIPSVMKKTIVLNGINVGTARLPVTEPT
                     GEVLEEIKRVVENYRTILNKSSENR"
     gene            12756..13388
                     /locus_tag="ACGI59_001962"
     CDS             12756..13388
                     /locus_tag="ACGI59_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketohydroxyglutarate aldolase"
                     /protein_id="HFG9739748.1"
                     /translation="MKKMNITKRMSECGALAIVREENLNRACEIAEGCIKGGITVIEM
                     SYTLNNAGEIIQGLNKKYGEILCVGAGTVLDSETARHAILHGAQFIIAPNYNEGVAKI
                     CNRYQIPYAPGCTSLTEAVDALSLGATFIKAFPISDFYGPKLVKVFKTPIPDMPVLAS
                     GGLNIENLHIWLENGVDVCGFGGLLTKGSVEDIAKNANKIREIIKNVRKI"
     gene            13865..16372
                     /locus_tag="ACGI59_001963"
     CDS             13865..16372
                     /locus_tag="ACGI59_001963"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFG9739749.1"
                     /translation="MKEDIRLHRMCKKEQATKNEVISEDILKSMSIGVSKHFLDDSFT
                     IIWGNTSFYQLLGYTEEEFLVKFSSLKDYYEQNLYHFESMKNHFLEAYHHGEKSIEYQ
                     ICISANNGDPVWVIMTGILIESQDNEKSMVYILYSKINELKIKQEELSDMEKESTENF
                     KWMMSEYAGNVYISDMDTYELLYLNKHSCNTLQASANELIGRKCYEVIQGRTSPCPFC
                     TNSYLKKDDIYEWEFYNKKLKRTFIIKDRMLNWQGRRVRIELSYDMYSAEYKLAKKDQ
                     ERESILKTIPAGMVRLDARDYLTILWCNDIFLNMIEYTKEQFAEELNNQCGYLFSEDY
                     KRSKKLAQDLKESGDNVVFEAKIYTRSREERIWTVTLCYISGEDSWDGIPSFYSIGID
                     ITKERKQIEKLQHISEKDALTGIYNRAETERQIKKYFEKNLNVMGALFMIDTDNFKQI
                     NDTEGHMIGDIVLTEMASGMKKIMRDSDVVGRIGGDEFTIFMKNISSVKDAEKKAEEL
                     LYMFRHLFQKEKSFLKVTCSIGIAIYPKDGTTFKEIYARADKALYQAKNMGKNNYVIY
                     NQDYFKELEEFDCSSLGTVIDSEKQYAEYPDNLTRYIFRMLYQTDDIDQAINMILEVV
                     GKQFDVSRVYIFENTEDGRYTSNTYEWCNEGISSQMAYLQNSNYQDYEDYEKLFEDEL
                     VFYCRDIHTLSPKLEELFSSQGIHSTLQCAYKEDRVFSGFVGFDECTGLRLWSQEEIS
                     TLSLISQIISMFLQRKKNNKLNQQIQQYQNILDNIDQCICVINKENNFLLYVNKKFKN
                     TYSKFQIGQPYYLDSIDIHKISIIWNKKEAYLCTSTI"
     gene            16714..18876
                     /locus_tag="ACGI59_001964"
     CDS             16714..18876
                     /locus_tag="ACGI59_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HFG9739750.1"
                     /translation="MKKIIYVLFTIELLIVSLLGLNIVKDNEINNILYNDTTSISVMF
                     KDYKKLNKNYSKWIKDIADENNVAISKYVFNNNEKLSIYTTDTSLNNNIKLKSGKFPN
                     TNSNEFISNINTNNSNQVGKFSTMSKDISILIRDFGKISEVGESGILYISTQNEDTIN
                     KILRELNVDRSIFVARLHDRYVNSNLYLNPSIIRDLILIILCFLATIIHYSISVSREA
                     SILRLNGYSKLDIIARVIKNILRIMILSSVTALLIYIIYILKLNIGVRACLYFAMFSI
                     ICILINILISILIVGFNSRSSKYVLSLNGKKSYGFVNIMHFTLKIVFVSFLILSINNC
                     ILNYNMLQTQLGNLSEWDKAKNVYNLTLKDTGEQSLGKEEVIKNAKIKGFYKNLVEEK
                     DAFLIDSTNFEKLEDGSYMYEINSKGKNPEVSQYGKNIKINKNYLKVNPIYSNGKEIF
                     NLIDYDDKTINLLVPKKLQVHENEIKKEFRELFYFEKIEVENMYNKELNRELNKTKKS
                     DLNVNIIYVDNNQSYFTYNSLVMDDNRNLIDDPIAIVDIDNIDDSFYLSYISRCVYFN
                     SKKLDALADISSIIEAQGVEAHIQSLHAVYNEYGLEINKLEKYLNSEIFTIIIIAISN
                     LMITYNIVASYYERNKYKLYIKKIFGYSTTARSMLLIVSLILTNIIPIGIISTRVDLS
                     NNIILFGLLILVIEVIVSIVLDKIISNSSFNKIIKGEH"
     gene            18881..19498
                     /locus_tag="ACGI59_001965"
     CDS             18881..19498
                     /locus_tag="ACGI59_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9739751.1"
                     /translation="MISLINISKKFKDKSIIDKFNLEISKGEFVAITGNSGSGKTTLL
                     NIMGLLEKPNSGDIIINNIKNPNSKQIRILQRDFYGYLFQNYALIENENVENNLKIAL
                     KYQKNINHNEKINYALESVGLRKYNKKKIYELSGGEQQRVALARVILKECEVIFADEP
                     TGNLDKKNRDIVFDILKSLNKNGKTIVFVTHDLDLSEQADRVIKL"
     gene            19687..20031
                     /locus_tag="ACGI59_001966"
     CDS             19687..20031
                     /locus_tag="ACGI59_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFG9739752.1"
                     /translation="MENLPNCPKCNSEYTYEDGTLLVCPECSHEWALGLGAEEDSNII
                     KDVNGNVLNDGDSVTVIRDLKVKGSSSSIKIGTKVKNIRLIHDSSDGHDIECKIDGFG
                     AMKLKSSVVKKA"
     gene            20601..21467
                     /locus_tag="ACGI59_001967"
     CDS             20601..21467
                     /locus_tag="ACGI59_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFG9739753.1"
                     /translation="MIDIHKNIYDNKLFEELKIDCKKCFGLCCVALYFSASDGFPINK
                     ESGKPCINLQPDFKCSVHNSLIKRGFKGCTAYDCFGSGQKVAQVTYKGIDWMQSPELT
                     NQMSEVFLIMRQLHEMLWYLKEASVLNISDTIKSKIDLIIEETEKITNMGPEQIINLD
                     IISHRTKVNLLLSQASESMMGKVKSFIKTSTLKNMKKLSKNIDLIGADLRKINLIGAD
                     LRGRFLIAANLRNTDLSGANLIGADLRDCDIRGANLENSIFLTQLQVNTAKGDSSTKL
                     PASLIRPKYWEK"
     gene            21580..21984
                     /locus_tag="ACGI59_001968"
     CDS             21580..21984
                     /locus_tag="ACGI59_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmaF family protein"
                     /protein_id="HFG9739754.1"
                     /translation="MSYQNYKCERDYVYDDYKYNKCNKCYNDYEEMTHVHEYSESVKL
                     AEECEDRHNHRAAGVTGEVIPINGGTNHVHKINDNVDFLDHFHKICVTTGPAIRIPGT
                     DKHIHLICGETTVNDGHCHKFLFTTQIEAPLV"
     gene            22148..22597
                     /locus_tag="ACGI59_001969"
     CDS             22148..22597
                     /locus_tag="ACGI59_001969"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFG9739755.1"
                     /translation="MKSGIILEMKQINSTEYDEMLQVWESSVRATHDFLTEKDIESLI
                     PLVEIGLKEVENIVCIKDNDIIKGFIGIDKDKIEMLFIEDKYRGNGIGKKLIKYAIDK
                     YNVKYVDVNEQNKKAVGFYIHLGFKVFDRSEIDGQGNPFPILHMKLI"
     gene            complement(22753..23130)
                     /locus_tag="ACGI59_001970"
     CDS             complement(22753..23130)
                     /locus_tag="ACGI59_001970"
                     /inference="COORDINATES: protein motif:HMM:NF018663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFG9739756.1"
                     /translation="MLTPYLIFNGTCEKAFNFYAEAFGGGKTIFARLDSNPNNPIMHA
                     SVTFTKYEGCIMGADTDKPVVISGMAICVVLPSREAIEEISVKLAEGGTLVQEFLPHP
                     PPHQNDGAAEVLDRYGYTWYLST"
     gene            23401..24309
                     /locus_tag="ACGI59_001971"
     CDS             23401..24309
                     /locus_tag="ACGI59_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2268 domain-containing protein"
                     /protein_id="HFG9739757.1"
                     /translation="MNIIEIRSDKIYKKIMDAPINKKEDIYRYELMKPFEFKWKCMNV
                     PIVARQKGGYDVIIASEMLGVLSPKDIDEKQKKNINVLSADKIWGTCKETIENSINAF
                     IKEGYDLNIKDYKYSILLANPNSPYTILSDGYWGDGGIPGYIFLSLVPNEYTINRLPV
                     LIAHECNHNIRFQFIEWNNNITLEEMMINEGLAENFATWMFGEEMLGPWVSRTDIETL
                     NTYIKPIIKSALKETGFQNITSYLYGDDIAKMQGYFPVGLPYCAGYACGYYMIKYYLE
                     KTNKSIIEATLLPYSEIIEAVKEFWE"
BASE COUNT         9538 a         2669 c         4263 g         8021 t
ORIGIN      
        1 tattaataag aataacttat atattccaat gaaaacgata ttggatttaa gtaagtttgt
       61 ctcataaaat gaaagtatat caattgataa tactttaaat aagaagggag aaaaaatgga
      121 agataaattt tatgcaaaag gcaacggaaa taacggatat attaaaaatc ttgaagtttg
      181 ttcctttaat aacttagatg gaacttgtgg aatgtttcaa atggctctgt acaaaagaga
      241 tgaaaaatac tatttatatg gatgctgttt tggaggaaat aaaaaaaatg gagtaatgat
      301 tagcgatatt acagaccctt ataatccaca atttataaaa cattttcaaa tgttagaccc
      361 taaagagtat cctacaacaa caactcccaa aattcaaata gcagatgatt taatgatagt
      421 agcaatgagt tgtggaagtg gaccaggagc acttgttgac caagctaaat tagcaaatat
      481 taagtgtgaa gcaggaatta gaatatatag tttaaaagaa gaccctttaa atcctaagtt
      541 tttaggatat tgggattgtg gcttaaagca tgtaatgggt gttcatagat tcatgtacaa
      601 tggtggaaga tatgtacatt tatcaagtga ttgtgttggc tttgaaggtc tgatttatag
      661 ggtcatagat ataataaatc ctactaatcc agtggaaata ggtaaatggt ggagaccaga
      721 ccaatatgca aatggatatc caaatagaac ttttgatgca ggagcacctc attgcccaga
      781 atttatggat aaaggatggc ttcatggacc tccatttgta agagacggaa aagcatattg
      841 tggttatgga ggagctggtt tagttgtatt agatgttgaa gatttaacaa gaccaagatg
      901 cttaggtgaa ttgccattta tgcctgcatt ttctagtaga cttgcaggtg caagaactca
      961 tacagcatta ccattgccag gaagagattt agtcgttgtt caaaatgagg gagaaagatt
     1021 ccagttcttt aaaccagata acattacaga tgttcaagct atgaataata tacatatggt
     1081 tgatgttagt gacccaacaa aaccaacatt aattgctcaa tttccatatc ctgaagttcc
     1141 aaaagatttc ccttatccta actttaatgt tgcgggatta ggaaaaccag ggccatttgg
     1201 cccacataat cttcatgaac caatggataa taagccatgg ttagagcaaa gaggagatag
     1261 agtatattgc tgttatttcc atgcagggct aagggtttat gatgtatcag acccatatta
     1321 tatcaaagag ctagcatatt ttataccacc aaatccaaat aaaacaccag aagaatctta
     1381 tttcccagga ttcccaggac cacgcttggc agtaacagaa gatcttatcg ttgatgatag
     1441 aggctacatc atcatagatg ctttagatga tggattctat atattaaaaa tgaaagaaga
     1501 ttaaataaat actaaagagg gagagttgaa aatgaaaata ctaggtattt cttttggaac
     1561 aaaaaatgga aataatgaca caatgtgtaa agttgcactt aaaggtgcca aagaagcagg
     1621 ggctgaagta gaatttattc aaatgtcatc tattaacatt aaacattgta ctggatgttg
     1681 tgcgtgtgtt aaaactttat tatctgggaa aggtagtatg tgtgtattaa aagatgattt
     1741 tgaatggttg cttgacaaaa tgaaagatgc agatggtata gtagtttcag accctatttt
     1801 tgaagaaggg gcatcaggat tattccatac tataatggac cgttttggac ccagagcaga
     1861 tagaggaaat aacattatag gaacaaaagt tgcagaagct ataggaggaa aaattccaga
     1921 ttcaagaatg cttaatgata aagtaatatc atttatggga ataggaggtt ctgactgggg
     1981 tacaagagtt caatgtgaac atgctatgct agctctcata ccaatgtgga aagttataga
     2041 taatgcttgg ttcccatggg caaaggaatt agtaatggat gatacaaggc ttgttcaagt
     2101 tcatcaaatt ggtttaaata tagttgaagc agcaaaagat tttgaaaaag cttcatatca
     2161 aggagaagaa ggtgtatgtc cacattgcca caatagatta ttctaccttg agccaggtac
     2221 taaaaaggca atttgtgctc tatgtggtat agttggagag ttagatacta taagtggaaa
     2281 aactatattt agttttccag aagagcaatt aggtcatgct catgatactt taccaggtaa
     2341 gtttatacat ggtgatgata ttaagaaaat ggaaggtcat tatgcagaag ttcgtaaaac
     2401 acaagaattt aaagaaagaa aaacatctta taattttata gagccattaa ctaaagaaaa
     2461 gcaagctgga gtttcattag gataataatt aaaatactaa gactcttttt taaggtatgt
     2521 tagatgttat aggaggctta ttgtatgaat gaaaagtcaa gttttaaaca aattgaaatt
     2581 attttagcag ttattttttt ggtacttaca tattacaagg ttagattaac tccagcattt
     2641 attgttatat ttataccact atatttaagt tattttgcag tatctatgat tgtaaatcaa
     2701 atagcaatta agctattgga gataaggcaa gctagggaag ctactaatta caatatagga
     2761 aatggataga aagaaaaaag aaatattaac actgtctatg gggattgctt tactacctcc
     2821 tttatgggca gtattagcac cttatatagg aataaaaact ggagctgtag cactaatctg
     2881 tgcaggatta tatgtaacta atggtaataa acaaaaagat ggattaaaaa taatgtttgg
     2941 tttttggtgt ggagatttat gggcagtgtt agccatttta attatgggat atatgaattt
     3001 caatcaaaac ctagaattat ttttaacact tagtatatta ggtttttttg cagttgtaat
     3061 tgcatcctta tttgaaaaaa taattttcct accatcatgg ctttgtggtt gggctatagg
     3121 tctaactatt atgacaggtg aaaatattat taatttacaa gatatttgta ttcaaattgc
     3181 tgtagctatg gcagtaggag tatggtacgt gggtgcaggt gtagatttgt ttacaatata
     3241 tattttgagt aaagatagga aaaagggaaa ttgaaagcat gtgaatatac tttgattata
     3301 ggagaatggc tatgggtttt gaatttaaaa taatgagaag tttaatatat gtaggacttg
     3361 ccaaggaaga atatagacct aagctaatgg actggttata tcgtcaccat attccagata
     3421 gtattagcac ttttggacca tattgtacta aatatgcctt ttatcaagca tatcctacac
     3481 caaatgaagg tgagcgtttt ggtgcacgta agatgcaact aacagaacat tattggcttg
     3541 tagatgaaca tatgcctgag atggcaaata gaattatgac agaatatatg cctatggatg
     3601 ttctacgttg gcaagggtgt ataccagatg tagaaaataa aagggttcat gaaaatgcag
     3661 aaagtggaga tgcaggacgt gcagtaggtg gagataatgg atgtccacca tttatatttg
     3721 cctttgttcc aataaactgg gaagaagact ttagaggaaa aggacgtact gtacaagatg
     3781 gaccaaacta tcgttggcaa tttatgatta agtatccaga tggtatctct aaagaagaag
     3841 gagaaaaatg gttctatgat gaggtagtgc catactttac aaactgttgc tatgttaatc
     3901 gttttgtcag tagtaaaata atgattaatt atggagcaac tgcttttgac cgtgtatcag
     3961 aactatggtt tgaaggggaa gaagaatggt ataaagctgt ggttgaagag acaaagtcgt
     4021 ttattaaaaa accagaatgg gcacaagaag aggagttccc atatttaaaa ccacaattca
     4081 atatcgcatc agtattctta ggtgatatag caactatgga tgcatactca cagtatcgtg
     4141 gatatatacc aatgagataa aaattatagt ttgtgataag tagaggaggc atttactctt
     4201 actggtagaa gaagacttat tgtatcctta aaagtaggtg aatatatagt tttatattta
     4261 aatttatata aaactaagac taataaatat gaaacaatag aattagaata atttgataat
     4321 atttgttaaa agagcattta gaatgtgttt aaagattaag atattccttt tatggtagca
     4381 gatagttgaa ataataaaac tgttatcatg aaaggaatat ttttatgtat aggaaatttt
     4441 taaaaataaa agttaaatat gtttaagaac atcttaaagt gaaagaatca ttgttagaaa
     4501 tatgtagaca atatagtctt tataacgtat catattaaca agtttacact tggattatga
     4561 aataaaaaga gtaattccaa aggttgtaaa tactatgctg actttgatac ttcaaagttt
     4621 aggtgatttt gtaattgtta atagaaatac gtttaatgag cataaagaat ttatctaata
     4681 ataggagaat ataatgtata caattggaca agtatctaaa tttttaggaa tatctagaga
     4741 tacactaaaa ttttatgagg acaaaggatt agtaaatcct aagaaaaatg atgaaaatgg
     4801 atatagaata tataatcagg ttgatatcta tgatattgca acaataaact tttatagaga
     4861 aattgatata gaaattaaaa agattcaaga aataagaaaa agtaagagta taaacaattt
     4921 agaactctta ctagaagaga aggaacaaat catactaaaa gagatagaat ataaaaagct
     4981 tctcttaaaa aaggttcagt tagtcaaaga agattgtgac aaaataaaaa catattttgg
     5041 aaaatacaca ataaaagaaa tgaagccttt ggaagtaaaa ggtaaaataa aacatgttac
     5101 taactatgag gaatatgaag ccttaaaaga gaatacaaac aacttaaaaa aagcagttac
     5161 attaactgat ttaagaagag taataaattt taatgaagaa ggtataatag aagaaaaatt
     5221 tataattgta agggaagtag atgactacaa caaaatcata gaaggtgaag ttctatctca
     5281 tcccaattgt atttatacta ttattgaaga tggcagatgg tcaactggag gagaaaatat
     5341 tgataataaa gtggaagaca gattaagaga aatagcaata gaaaatgaat gtgaattact
     5401 tggaatagtc tatgttaata tattgcttac tacttatgaa gaagggattg agcgtatatt
     5461 tttagagatt tatgcaccta taaaataata taaaagcatt gacctgggac ttactcatag
     5521 gtttaaactc atgatataag cagttataac ttatatagca attaaatatt aatttgaaat
     5581 gaggattact tatgagaaca gagaaatcat taaaaaggcc tatagccagt tttattattc
     5641 taacaaatat aattttttta ccactttttt tattggtagt agtgactata atgttaaaac
     5701 ttccaacagt gatatctgac attgcgttgt gtatatcagc gtggtcatcc acttttgcat
     5761 ttatgatatt atttaaaaaa atttatccag gtaagaaatt tttagtacac gtaaaagata
     5821 gatttagaaa taaacttaaa ttttcaacag tatctataat aattagtata caagtcctaa
     5881 tagctgtagt agttatattt atattagcat ctaaaaatca tggaataatg cctaacttta
     5941 cagtttcatc atggggagca tttatatatt tatttttaaa aaatttattt gcaggatcat
     6001 taggtgagga attaggttgg agaggatttg ttcaaaatca tcttcagaaa agacattctc
     6061 ctttaaaagc atccataata gtaggttttt ggtgggggat gtggcattta ccaatatggt
     6121 ttacaacagg attcaaagga cttgatttaa taaaatatat tatatttttt atgattgcaa
     6181 ttatatcaat cagtattata atgacaactt tttataatct aaataaaact ttgataatac
     6241 ctattgtaat acatcaacta tttaattttt taatagggat aattaatgga gatttaatag
     6301 agcttataaa ttactatgca atgttatatt taatatttgc aattgcatta atagttataa
     6361 atccaggaaa tgtattatat aaaaatatta gagagtaata tatatcttta tggtaagata
     6421 taaagatatt attagaagaa catccaagga ggaataaaca tggttttact gattgttgat
     6481 gcacaaaagt taataacaaa cgagaggtta tataaattta atgaatttgt agtaaatgta
     6541 gaaaatttaa ttgatacagc tagaaaaaat aatatagaag taatatatgt acgtcatgat
     6601 gatggagttg aaaatgaatt gactaaaggt aaaaatggct ttgaaatata cgaaaaattt
     6661 aaaccatgta atggagaaaa aatatttgat aaaaaagtaa atagtgcttt taaagaaact
     6721 ggtctattag aatacctaac aaataatggt gaaaaagata ttattattgc tggtctccaa
     6781 acagattatt gtattgatgc cactataaaa tgtggatttg aacatggatt taatataata
     6841 gttccagctt attcaaatac aactgttgac aattttttta tgtcagcaga acaatcatat
     6901 aaatactaca atgaatttat atggaatgaa agatatgcga agtgtatttc attagaggaa
     6961 acacttagaa aaatgaaata aaaatagagt atgaattgta tatgtacata agttgatttc
     7021 ataaaaaagg atgttaaatt ccaagttaaa aattagatta aatatattat attatagata
     7081 aggcacatca attttatttt aaattaatgt gctttttaat tgtagtaatt tatttataat
     7141 caatatgatt tttattattt aaatcaattt ttcttgagtt tctttgtcta aagaaacttt
     7201 ttttatttat aaaatctaca gttatattat agtatctatt ttagagaagt ttaatttttg
     7261 tatcaatcta aaacaaaagt tatacttaaa tgaaattgaa aacgataaaa ttctcaaata
     7321 aaataagtat tgtggaggtg atgaaagaat ccaatgataa tatgaagact aatcttaata
     7381 agaaagtagg ggttaatggt agtggacaaa gaaattgtat caattctcca gattttatta
     7441 aatagcacat tgattacaac taatggatta caagaggagt ctaattcatc aaagagacaa
     7501 attacttata gaataaataa aatcaatgac atgcttaaag taaaaaatgt accacttata
     7561 tgtttaagag ctgataaaga tattatcgtt agaaaagaaa caaaaaaagc aattaaagaa
     7621 attttagaaa aaaactattc taagaatact tattatttta gtaagacaga aagactttta
     7681 tatatgtatt taatgctttt tattaacatg gatgatattt caattaatca ttttataaat
     7741 tctataaaag taagtaggtc tactgtaaat cttgatttca aggatttgat accagagctt
     7801 gaaaagaaga acattaaagt taagaacaat cgtataaatg gatattacat agttggcaat
     7861 gagatggaaa ttaggagagt gcttattaaa aatattatag aaactttgtc taatgagaaa
     7921 agttcaagag tatttgatat ttttattaaa gaatataatt tagatagttt tgaagaggct
     7981 aaaaaaatta tattaaagct aataaaaaaa tatgaaatta cttttgttga agatagatta
     8041 gtagaattta tatatatatt tatctttcta aaggcaagga tgtattcaac tagaatagtt
     8101 actcaggaaa atgtagatat tcctaatatt tcagttatga gttcaatgaa ggagtataaa
     8161 tttgctaagg aattattaga agtatatggg agtgaagata agataaagcc tcacaatata
     8221 atgtatatta gtgcttggat tttaggtata tcagtaggaa attatgaaga agatacagaa
     8281 gataaggatg taatttcaaa aattgtaaat aaaatgatga caaagtttgg atatttaagc
     8341 ggagtttact atataagcca agaaaaaata tttaaacaat tatattctca ttttagacct
     8401 gcctactata gacttttatt taaattacct atttataatc cgatttgtga aaaagtaaaa
     8461 gaagaatata aattagttta tagaattgta agtaatgcaa tgaaagagtt ttgcggatta
     8521 tttggagaag aaattcagga agaagagtta gcatatctta caatgcactt tgcaacactt
     8581 ttttcaaata aaaaagaatt tgatggtttt agaaaaagaa tagcattaat agtttgttca
     8641 aatggcgttg gtagttcagc tattttacat gcagaactta caaacttatt tcctgactta
     8701 cactttatac caatgtcgga atcagttgga ttagaaaata taagtgaacc tgtagatatt
     8761 gtatttacta ctaattataa cgcagagggt ttaaaaacag atgtaccagt aattaaagta
     8821 agccctgtta tgaccacaaa agaaaaatat aagattacac gtgaagtgta tattcaatta
     8881 ggtgatattt ttttaagaca acctaaaata gatgaggtta tggatataat aaaaaaatat
     8941 gcaaatgtaa cctctgaaag tattctttca agtgagctat tagcttattt tacacagata
     9001 gagaatttta catctaagga aggagagggg cctatgctaa gtgagattac taatgaaaca
     9061 ctagtaaaaa taaaggtaaa agctaagaat tgggaggaag ctattagaca gtcagcttct
     9121 gtattagttg aaaataacaa agtaactgaa gggtatgtag atgctatgat taactcagca
     9181 aaagcatctg gaccatacat agtaattaca aaacatgttg cacttccaca tgctagacca
     9241 gaagctgggt caaaagagat tgcaataggc attgctactt tagatagtcc tattgagttt
     9301 gggaataacg aaaacgaccc tgtaaaatat gtattttgtc taagtgcaat agacaataat
     9361 agccatttac gtgctatgtc tgagctagtt gaactattgg aagaagatga gttttttaag
     9421 gttttagata gtgctaaaga agctaaagaa attattgatt ttataaaaac acacgaatta
     9481 taaaggagga tttaattatg tataaaattt taatcgcatg tagagcagga gttggctcaa
     9541 gtttaatgtt aaaaataaag actcaacaaa ttattaagga aaataacttt ccaattgaag
     9601 tagaacatgg ttcattggat tcactaaatg gattcaatgg agatgctgta gaaacattaa
     9661 ttgatgttgc tgaagaatta aaaaataaaa atttgaagtt tgatataatt gggattcaca
     9721 atattgttga ccgtaatgaa ataaagacag cactagaaaa attccttgct tcaaaagagg
     9781 gataaattta agatgataag gaggaatatt tatggagttt attattagtt tgctgagtac
     9841 acctgctgtt ttgcttggcg tagttgcatg tataggattg attcttcaaa agaaatcagc
     9901 tgttgaagta tttacaggct cttcaaaaac acttatcggt tttttgatat ttggtattgg
     9961 tgcctctgct atgacaggag cacttcaaaa tttcaataaa ctgttccaac atggctttgg
    10021 aattactggt gttatagctt caccagaagc agctacagca cttgcacaag gtacatatgg
    10081 ttttgcagtt tcatgtacat taattcttgg attcatatta aatttagtat ttgcacgtat
    10141 tacaaagttt aagaatattt tctttacaac aggtcatagt ttgttctttt cttgtgtatt
    10201 agttttaatt atgaaagcac atggatttga taatacaatc actattttaa taggtggaac
    10261 tatcttaggg tttatgtctg ctgcactacc tcaattctgt caaccattta tgcgtgaatt
    10321 aacaggtggc gacgaacaag ctattggtca ctttaacatg attggttatg gtttatcagg
    10381 ttatattggg cgtttatttt caaaacataa agatgataca acagagacta tagaatttcc
    10441 taagtggtta tcaatattca gagacttctt aatgggtcta tccatagtta tgttaatctt
    10501 attttatatt gcaacattaa aagctggtca agcatttact caagaaattg ctggttcaac
    10561 acattggttg gtttttccgc ttatagaggc atttacattt gtggcaggta tgtcaatatt
    10621 gatgtcagga gttcgtatgt tcttagctga aataactgca gcatttgtaa tcatttcaga
    10681 aaagtatatt ccaggttcac gtcctgcgct agatgttcca accgtatttc catatgcacc
    10741 aaatgcagtt attattgggt ttatatcagc atatgctgct ggtctattag caattgctat
    10801 tatggcatca tttcctagta tatttcctgt agtaattatt ccagctgctc acatatgttt
    10861 cttctcagga ggtacagctg caatatttgg caatacttca ggaggatgga gaggggctat
    10921 tgctgggtca tttgttgtgg ggttattact tgcattttta ccagttatac tatatccagt
    10981 ttatgggact cttggtatag aaggagcaac tttccctaat attgattaca acgtagttgg
    11041 ttcgatatta cataatattt tacaatttat aaaacctatt tagactaaat tatataaaag
    11101 tatagtcata gttactatga tttcaaggag gatacatctt atggaaaata cattatctga
    11161 atttttagat aatatatcta atgaattgtc agaatttatt gcctcaatag atgagacatc
    11221 tataaataaa gcatgcgaat taattcttga agctgaaaaa aatcacggac gtgtacatgt
    11281 gactggtatt ggcaaaccag gacatgtatc gggatatatt tcatcattgc tttcatctac
    11341 tggaacgtca gcatatatat tgcatggtac agaagcagta catgggtcaa gtggtcaagt
    11401 agttgaagga gatgttgtta ttgcaatatc aaatagtggt gaaactcaag agttaaaagc
    11461 aactcttaag actctaaaag taaatggagc aaagataatt ggtgtaagtg gtaatgagag
    11521 ttcattttta aaaaatattt ctgatatatt cctgtttgca ggtgttaagc aggaaggaga
    11581 ttgtctcaat aaagctcctc gtgcttcaat tttagctgaa acaataatat tacaatcatt
    11641 aagtgttgta ttacaatatg ctaaaggtct taatacacag gagtatttga agtggcatcc
    11701 agcaggaagt cttggaaagt ctattagaga gggaatataa ttttatgaaa tataaaggga
    11761 ttatatgtgc aatgataaca ccatttgatg aaaatcaaaa tattaattca caagcaacat
    11821 gtcaattaat tgattatttg attgaaaagg gaatttatgg attatttatt ttgggcacta
    11881 atggtgaatg tcatgtatta acagatgatg aaaaagttga atttgcaaag attgttatta
    11941 atcatacaaa taatcgtgta cctgtttttg ttggaacagg gggtaattca actcgtgaag
    12001 ttgtagattt atctaagaga atggaagcga ttggagcaag tgcattatct attattacac
    12061 catactttgt tacaccaaca caacaggaat taatcttaca ttacaaaact attgctaatt
    12121 cagttaattt acctattata atgtataata tgccaggtaa gactggaatt aatattgaac
    12181 cagagtcagt acgtgaatta tcaaagataa agaatattgt tggaattaag gatagtagtg
    12241 gtaaacttga caacatgaaa gcttatattg aagcaacgaa gaatgagaat tttagtgtgt
    12301 tttcaggctc agattcactt attctggata gtctaaaagc tggtagccaa ggagctgttg
    12361 cagcaacaac aaacttcctc acagagattg gtatctctat ctatgaaaac tttatgaaag
    12421 gggatttgga agaagctcaa aaatctcaaa gtagtataga agaacttcga cgtatactga
    12481 aacttggaac aattccatct gttatgaaga aaactattgt cttgaatgga attaatgttg
    12541 gaactgcacg acttcctgta actgaaccaa ctggggaagt acttgaagaa attaaacgag
    12601 tagttgaaaa ttataggaca atacttaata aaagtagtga gaatagataa tataaatagg
    12661 gaaattacac tggtatataa ttaatatata tattataaaa catgactaaa attaatgaat
    12721 aagtaaagtg gtttgatgag tatagaggtg ataatatgaa aaagatgaat ataaccaaaa
    12781 gaatgtcaga atgtggggct cttgcaattg taagagaaga aaatcttaac cgtgcatgtg
    12841 aaattgctga aggatgtata aaaggtggta taacagttat agaaatgagt tataccctaa
    12901 ataatgctgg agaaataatt caagggctta ataaaaagta tggtgaaata ctttgtgtgg
    12961 gagcaggaac agttttggat agtgaaacag ctcgtcatgc tatattacat ggagcccaat
    13021 ttattattgc accaaattat aatgaaggtg tagcgaagat ttgcaataga tatcagattc
    13081 cttatgctcc aggatgtaca tcattgactg aggcagtaga cgcactaagt ttaggagcaa
    13141 cttttataaa agctttccca atatcagatt tctatggacc taaactagta aaggtattta
    13201 agacaccaat acctgatatg ccagttttag caagtggagg gcttaatatt gaaaacctac
    13261 atatatggtt agaaaatgga gttgatgtat gtggatttgg tggtttatta actaaaggtt
    13321 ctgttgaaga tattgctaaa aatgctaaca agattcgtga aatcattaaa aatgtaagaa
    13381 aaatttaaca tagggctgta tcatagtatc ttataattac tatgatacag cctttttaat
    13441 attattcaaa tttaaaacag tttacatttc tattatatta catataattt cagtaatttt
    13501 atcttaattt ttctatctgg aaaaaaatga tttaaaactt aagttatgat ataatttcat
    13561 taaaagaagg cgatttttag taaatatgag aaaataatgt gtttttaggg gaaaagttaa
    13621 atacagattt aagtgaacaa gaatcgataa ttaagatatt aaatggtgaa tagggcatca
    13681 aaaaaattac taaattaaaa gttttagagg gagaatctta gcaagtgtgt gtgaaaattc
    13741 aatttcaaac aaaaataatt gtaaatgaaa tcaatttata cacgttgaag attcactttt
    13801 atcattatat taaaaagcag agtgaattaa aagtgagtct aaagatagga ggataattaa
    13861 aacaatgaaa gaagacatta ggttacatcg aatgtgtaaa aaagaacaag caacaaagaa
    13921 tgaagttata tcagaggata ttctaaaatc tatgagtatt ggggtcagta aacatttttt
    13981 agatgattca tttacaataa tatggggaaa tacatcattc tatcaattat taggatatac
    14041 agaagaagaa tttttagtta aattttctag tttaaaagat tattatgaac agaatcttta
    14101 tcattttgaa tctatgaaaa atcatttttt agaggcttat catcatggcg aaaagagtat
    14161 agaatatcaa atttgcattt cagcaaataa tggagaccct gtttgggtaa taatgactgg
    14221 tatattaatt gaatcacaag ataatgagaa atcaatggtc tatatattgt attcaaaaat
    14281 taatgagtta aaaataaagc aagaagaact atctgatatg gaaaaagaga gtacagagaa
    14341 ttttaaatgg atgatgtcag agtatgctgg gaatgtttat attagcgata tggatacata
    14401 tgaattacta tacttaaata agcactcatg caatacttta caggcatcag caaatgaact
    14461 tattggaaga aaatgttatg aggtaattca aggtagaact tcgccatgtc cgttttgtac
    14521 aaattcgtat ttaaaaaaag atgacatata tgaatgggaa ttttataata aaaaactgaa
    14581 acgaacattt ataattaaag accgtatgtt gaattggcaa gggcgaagag ttcgtattga
    14641 attatcttat gatatgtata gtgctgaata taaattagca aaaaaagatc aggaaagaga
    14701 atcaattttg aaaactattc cagcaggaat ggttagatta gatgcaagag attatcttac
    14761 tatactttgg tgtaatgata tatttttgaa tatgattgaa tatacaaagg aacagtttgc
    14821 agaggaactt aataatcaat gtggatattt attttctgaa gattataaac gtagtaagaa
    14881 actggcacaa gatttgaagg agtctgggga taatgttgta tttgaagcaa aaatatatac
    14941 acgttcaagg gaagaacgta tatggactgt tacattgtgt tatatcagtg gggaagacag
    15001 ttgggatgga ataccatctt tttatagtat aggtattgac ataacaaagg aaagaaaaca
    15061 gattgagaaa ttacagcata tatcagagaa agatgcatta acaggaattt ataatcgtgc
    15121 tgagactgaa aggcagatta agaaatattt tgaaaaaaat ttaaatgtta tgggtgcact
    15181 gtttatgatt gatacagata attttaaaca gattaatgat acagaagggc atatgattgg
    15241 agatatagta cttactgaga tggcatcagg tatgaaaaaa ataatgcgtg acagtgatgt
    15301 tgttggaaga ataggtggag atgagtttac aatttttatg aagaatattt cctcagttaa
    15361 agatgcagaa aagaaagcag aagaactatt gtatatgttc cgtcatctat ttcagaaaga
    15421 aaaaagtttt ctaaaagtaa cttgtagcat aggaatagct atatatccaa aggatggtac
    15481 tacattcaag gaaatatatg caagagctga taaagccctg tatcaagcaa aaaatatggg
    15541 aaaaaataat tatgtaatct acaaccaaga ttattttaaa gaattagaag aatttgattg
    15601 ttcttctctt ggtacagtta ttgattcaga aaaacaatac gctgaatatc ctgataatct
    15661 tacacgttat atattccgta tgctttatca gactgatgat atagaccagg cgattaatat
    15721 gatacttgag gttgttggga aacagtttga tgtaagtcgt gtttatattt ttgaaaatac
    15781 agaagatggt agatatactt ctaatactta tgaatggtgt aatgaaggaa tctcttctca
    15841 aatggcttat ttgcaaaaca gcaattatca agactatgag gattatgaaa agctttttga
    15901 ggatgaatta gttttttatt gtcgagatat ccatacatta agtcctaaac tagaagaatt
    15961 gttttctagt caaggtatcc actctacact tcaatgtgca tataaagaag atagagtttt
    16021 cagtggattt gttggatttg atgaatgcac aggactacgt ctatggtcac aagaggaaat
    16081 aagtacatta tcattgattt ctcaaattat atcaatgttt ttacaacgta aaaaaaataa
    16141 taaacttaat caacagattc aacaatacca gaatatttta gataatattg accagtgtat
    16201 ttgtgtgatt aataaagaaa ataatttttt attatatgta aataaaaaat ttaaaaatac
    16261 atattctaaa ttccaaattg gacaacctta ttatcttgat tctatagata ttcataagat
    16321 atccattata tggaataaaa aagaagcata cctatgtact agcacgattt aatggtaaag
    16381 taaagtttat attggactta aattaaattt cgttttaaat aagatattat tttagttgct
    16441 tactgaaaag actagaatct atattaatga attatactaa ttttatacca ctattaaaat
    16501 attgtttgct aaaaataaaa aatagcctta aaacagttga ttataaaaaa ttaactgctt
    16561 taaggctatt tttatctttg tgacgaaata gacacttgcc atttagaatg tgacaatata
    16621 gtagttgttt agaaaaggca caaagcatta tactttaact aaagtaaaat gtattttgct
    16681 tttagaataa aaacaattag gagggagcta taattgaaaa agataatata tgtattattt
    16741 acaattgaac tattaatagt atctttatta ggtctaaata tagttaaaga taatgaaatt
    16801 aataatattt tatataacga tacaacaagt atatctgtaa tgtttaaaga ttataaaaag
    16861 ttaaataaaa attatagtaa gtggattaaa gatattgcag atgaaaataa tgtggctata
    16921 tcaaagtatg tttttaataa taatgaaaaa ttatctatat atacgactga tacaagtttg
    16981 aataacaata taaagttaaa atcaggaaaa tttcctaata ctaattcaaa tgaatttatt
    17041 agtaatataa acactaataa tagtaatcaa gtaggtaaat tctcaactat gagtaaagac
    17101 atatcaattt taattaggga ttttggtaaa ataagtgaag ttggagaatc aggaatctta
    17161 tatatttcaa ctcaaaatga agatacaata aataaaatat taagagaact aaatgtagac
    17221 agaagtattt ttgtagcaag attacatgat agatatgtta actcaaattt atacttaaat
    17281 ccttcaataa ttagagattt aatactaatt atcctttgtt tcttggctac aataatacat
    17341 tattcaatta gtgtgagtag agaggcttct attttaagat taaatggata ctctaagcta
    17401 gatataattg ctagagttat aaaaaatata cttagaatta tgattttatc atctgtcaca
    17461 gcattattaa tatatataat ctatatttta aaattaaata taggtgttag agcatgctta
    17521 tactttgcta tgttttcgat tatatgtatt ttgataaata ttttgatatc tatattaata
    17581 gttggtttta atagtagaag ttctaaatat gtgctatctc taaatggaaa aaaatcttat
    17641 ggttttgtaa atataatgca tttcacattg aaaattgtat ttgtatcatt tcttatatta
    17701 tccataaata actgtatttt aaattataat atgctacaaa ctcagttagg aaatctatct
    17761 gagtgggata aagcaaaaaa tgtttataat cttactctta aagatacagg cgaacaatct
    17821 ttaggaaagg aagaagtaat taaaaatgcc aagattaaag gtttctataa aaatttggta
    17881 gaagaaaaag atgctttctt aatagattca actaattttg aaaaacttga agatggaagt
    17941 tacatgtatg aaataaattc aaagggtaaa aacccagaag taagtcaata tggtaaaaat
    18001 ataaaaataa ataaaaacta tcttaaagtt aaccctattt actcaaatgg caaagaaata
    18061 tttaatctaa tcgattatga cgataagact ataaatttat tagttcctaa aaagttacaa
    18121 gtacatgaaa atgagattaa aaaagagttt agagaattat tttattttga aaaaatagaa
    18181 gtagaaaata tgtacaataa agagttaaat agagagttaa ataaaacaaa gaagtccgat
    18241 ttaaatgtta atataatata tgtagataat aatcaaagct attttactta taactccctt
    18301 gttatggatg acaataggaa tctcattgat gacccaatag caatagttga catagataat
    18361 attgatgatt ctttttatct ttcttatata tctagatgtg tatattttaa ttctaagaaa
    18421 ttagatgccc ttgcagatat aagtagcatt attgaagctc aaggtgtaga agcgcatatc
    18481 caaagtttac atgctgttta taatgagtat ggtttagaaa taaataaact agaaaaatat
    18541 ttgaatagtg aaatttttac tataataata atagcaatat caaacttaat gattacctac
    18601 aatatagtag ctagctacta tgaaagaaat aaatataagt tatatattaa aaaaatattt
    18661 ggttactcaa ctacagctag aagtatgttg ttaatagtct cattaatatt aacaaacatt
    18721 attccaatag gcattatatc aacaagagta gatttaagta ataatattat cttatttgga
    18781 cttctaatat tagttataga agtaattgta tctattgtat tagacaagat tatctcaaat
    18841 agttcattta ataaaattat aaaaggagaa cattaaaaag atgatttcat taataaatat
    18901 atctaaaaaa tttaaagata aatctataat agacaaattt aacctagaaa tttctaaggg
    18961 cgagtttgta gcgataacag gaaatagtgg ttcaggtaag acaactttac ttaatataat
    19021 gggattatta gaaaaaccta attctggtga tattataata aataatataa aaaatccaaa
    19081 tagtaaacag ataagaattt tacaaaggga tttttatggc tatctatttc aaaattatgc
    19141 tttaattgag aatgaaaatg ttgaaaataa cttgaaaatt gctttaaagt atcaaaaaaa
    19201 tatcaatcat aatgaaaaaa taaattacgc acttgagtct gtaggtctta gaaaatacaa
    19261 taaaaaaaag atatacgaat tgagtggagg agaacaacaa agggtagcat tagcaagagt
    19321 aatattaaaa gaatgtgaag taatatttgc agatgaacct acaggtaatt tagataaaaa
    19381 aaatagagac atagtatttg atatacttaa aagtctaaat aagaatggta aaactatcgt
    19441 atttgtaact catgatttag atttatcgga gcaggcagat agagttataa aattgtaata
    19501 atataaacta taataaatat aagaaagaga ttacagctaa attaagttta ataataatac
    19561 gaatagaatt taaacattaa gtaaattagt agtttgtaaa tataagtata atatgatatc
    19621 ataaaggctc aaaagaaatt ttatataata agaaatctaa aaataacaaa tggaggacat
    19681 ataaagatgg aaaatttacc aaattgcccg aaatgtaatt cagaatatac ttatgaagat
    19741 ggaactcttt tagtttgtcc agaatgctct catgagtggg cattaggatt gggagctgaa
    19801 gaagattcaa atattatcaa agatgtaaat ggaaatgtat taaatgatgg agattctgtt
    19861 acagtaatta gagaccttaa agtaaaagga agttcatcat ctataaagat tggaacaaaa
    19921 gtgaaaaata tccgtttaat ccatgactca tcagatggac atgatataga atgtaagata
    19981 gatggttttg gagctatgaa gctaaagtct tctgttgtta aaaaagcgta atattataaa
    20041 taagctgtag tgtttattgt ttttcagtac agtataaaac cataagaatc tattaagtaa
    20101 taaaagtttg tttatttatc tttatatttt cttatggttt tattaaatat aaattttatt
    20161 gaacaccaaa atattctctc ctctacaaat aagccaatct tatttaaact aatatacttt
    20221 gtactattta tactgaaatt gtaaatttaa atgctatgtt atttaggttt gtatagttaa
    20281 tttatatatt tctgatttta taaattgtat ttattattat atcttgattc taaaatcatt
    20341 aaaaatttca acttctataa agatttacaa tttatcaaat acattatgag tatataaaca
    20401 atttattatt acagtgctac atttaaaata agtcttagta taaaggtatt gttacaagta
    20461 taaagaaact tatgtatgct attggaataa ataatacctt aaattataat taagaataag
    20521 aactgttaaa taagggactt gattatcatt tagttataga taatataata ttaaaaataa
    20581 agtttaagag gagaaaaaat atgatagata tacataaaaa tatatatgat aataagttat
    20641 ttgaggagct aaagattgac tgtaagaagt gctttggact ttgctgtgta gctctttatt
    20701 tttctgcttc agatggattt cctatcaata aggaatcagg aaaaccctgt attaatttac
    20761 aaccagattt taaatgttct gtacataata gtcttattaa gcgaggtttt aaagggtgta
    20821 ctgcatatga ttgttttggt tcaggtcaaa aagtagcaca agtaacatat aaggggatag
    20881 actggatgca atcaccagaa ctaacaaatc aaatgtctga agtgttttta attatgagac
    20941 aattacatga aatgttatgg tatcttaaag aagcatctgt attaaatata tcagatacaa
    21001 tcaaatcaaa aattgattta ataattgaag aaactgaaaa gataactaat atgggcccag
    21061 aacagataat taatttggat atcatatcac atagaacaaa ggttaattta ttattatcac
    21121 aagcgagtga gtctatgatg ggaaaggtta aatcatttat caagacttct actcttaaaa
    21181 acatgaaaaa attaagtaaa aatatagatt taataggagc agaccttaga aaaataaatc
    21241 taataggagc agatttaaga ggtagatttc taatagcagc aaatttaaga aatacagatt
    21301 tgagtggggc aaatttaata ggagcagact taagagattg tgatattaga ggtgctaatc
    21361 ttgaaaatag tatattttta acacaattac aagtaaatac tgcaaaagga gattctagta
    21421 cgaagttacc agcttcacta atacgcccta aatactggga aaaataatta aattaataca
    21481 taattttaca aaatatgaac ttttttgact ttaattcata ttatacatta gagattcttt
    21541 acaagaattt cttaatataa tatagggaga gatataaaaa tgagttatca aaattataaa
    21601 tgtgaaagag attatgtgta tgatgattat aaatacaata agtgcaacaa gtgctataat
    21661 gattatgagg aaatgacaca tgttcatgaa tattcagaaa gtgttaagtt agcagaagaa
    21721 tgtgaggata gacataacca ccgtgcagca ggggttactg gtgaagttat accaataaat
    21781 ggaggtacaa atcatgttca taaaataaat gataatgtag atttccttga ccatttccat
    21841 aagatatgtg ttacaacagg gccagcaatt agaatacctg gaactgataa acacattcac
    21901 ttaatatgtg gagaaactac tgttaatgat ggtcattgtc ataaattcct ttttactact
    21961 caaatagaag cacctttagt ataagctgat gtgaattatt gtagtgaatt tgctagatta
    22021 tagtataata tataatagat aatatttaaa cttaatgagg actatgatat ttaaccttcg
    22081 tagtcctttc taagttttgt ataaaaaagt gttatgcaat taaaagtgat atagggagga
    22141 aatttttatg aaatctggaa taattcttga aatgaaacaa ataaattcaa cagaatatga
    22201 cgaaatgttg caagtctggg aatcatcagt aagagcaacg catgattttt taacagaaaa
    22261 agatattgaa tctttgatac cattagttga gattggactt aaagaagtag aaaatatagt
    22321 ttgtataaaa gataatgata taataaaggg ctttatagga atagataaag ataaaattga
    22381 gatgctcttt atagaagata aatatagagg aaatggtatt ggtaaaaaat taataaaata
    22441 tgctatagat aaatacaatg taaaatatgt tgatgtaaat gagcagaata aaaaagctgt
    22501 aggattttat attcacttag gatttaaagt gtttgatagg tctgaaatag atggacaagg
    22561 gaacccattt ccaatactac atatgaaatt aatttaaagt tatacttccc acttattcgc
    22621 aaaaatattt acttaaattt ttatacaaat aaaagtcgtg acagtcttag gacacgactt
    22681 ttttactaca ctattaaatt gaaatttatc tgttctatat tatgattatt agatgtagtt
    22741 tttttatttt aactatgtac ttaaatacca agtataccca tacctatcaa gtacttcagc
    22801 agcgccatca ttttgatgtg gtggtgggtg tggtaaaaat tcttgtacaa gtgtaccacc
    22861 ttcggcaagt tttacagata tttcttctat cgcttctcga gatggtagaa caacacaaat
    22921 cgccatgcca gaaattacaa caggcttgtc tgtatccgca cccattatac aaccttcgta
    22981 ttttgtgaaa gtaacacttg cgtgcataat aggattgttt ggattgctgt ctaatcgcgc
    23041 aaatatagtt tttcctcctc cgaaagcctc agcataaaaa ttaaatgctt tttcacaagt
    23101 accattaaat attaaatatg gagttagcat gaccaatata tcctccttga tatagaataa
    23161 tttatagttg cacctgataa tctaaattat gatttagtaa attgtatcat agggaaacct
    23221 tgaagtatat aattttttat tattatatta tacaaattta ttaatttaaa aaaatgttat
    23281 atgtacggaa ggtttacagg ataaaatata ataaaaagat taaggtgaca ttataaaaaa
    23341 tgtatattga cattgacgct acgtaattgt atatactttg tcttaaagga gatggtaaat
    23401 atgaatatta tagaaattcg ctcagataaa atatacaaga agataatgga tgcaccaata
    23461 aacaaaaaag aagatatata cagatatgaa ttaatgaagc cttttgaatt taagtggaag
    23521 tgtatgaatg ttccaatagt tgctagacag aaaggtggat atgatgtaat tatagcaagt
    23581 gaaatgttag gggttttatc gcctaaggat attgatgaaa agcaaaaaaa gaatataaat
    23641 gtgttatctg ctgataaaat ttggggcact tgcaaagaaa ccatagaaaa ctctataaat
    23701 gcttttataa aagaagggta tgatttaaac attaaggact ataaatattc aatattattg
    23761 gcgaatccaa atagtcctta tacaatatta agtgatggat actggggtga tggtgggatt
    23821 cctggatata tatttctatc attggttcct aatgaatata ctatcaatag attaccagta
    23881 ttaatagcac atgaatgtaa tcacaatatt agatttcagt ttatagagtg gaataataat
    23941 ataacattag aagaaatgat gataaatgaa ggtcttgcag aaaattttgc aacatggatg
    24001 tttggagagg aaatgttagg accttgggtc agtagaacag atatcgaaac attaaatact
    24061 tatataaagc caataataaa aagtgcttta aaagaaactg gatttcaaaa tataacatct
    24121 tatctttatg gtgatgatat agctaaaatg caaggatatt ttccagtagg gttgccttat
    24181 tgtgcaggat atgcttgtgg atattatatg attaagtatt atttagaaaa gacaaataaa
    24241 tcaataatcg aagcgacttt attgccttat agtgagataa tcgaagcagt aaaagagttt
    24301 tgggaataaa ttttataaat tgcataattt gaaataaatt atgcaattat tttattttta
    24361 agaaaaaata aattgacaat aatttataag aaatatacaa taatatatat aaagaaatat
    24421 ttcaatattt aaaagtttta agatttcata tctagagtag ctaacattac atgttagttt
    24481 cttgttactt a
//
LOCUS       DAYAPA010000053        23942 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.053, whole genome shotgun sequence.
ACCESSION   DAYAPA010000053 DAYAPA010000000
VERSION     DAYAPA010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23942
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.053"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(<1..338)
                     /locus_tag="ACGI59_001972"
     CDS             complement(<1..338)
                     /locus_tag="ACGI59_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HFG9739758.1"
                     /translation="MIINRSKDSSSNEISFVSKDMGFLLTQSEVSYNFKDKLVEDIAK
                     QVFAENRLSVGTIAKTNVKYTKMFIGVNGYDTIMSAYTEASKKTKKKYMIEANLDKFN
                     VIEKGTVTLNV"
     gene            complement(620..1042)
                     /locus_tag="ACGI59_001973"
     CDS             complement(620..1042)
                     /locus_tag="ACGI59_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate protein"
                     /protein_id="HFG9739759.1"
                     /translation="MEMWLRQAEDRFRFPVFPSSFSINGKAAVNSSSILKIGEIATFG
                     GVALKSISISSFFPNKDYTFCDYTGFPSPYDCVNKIEKWMKEGFILRFTITETNINME
                     VIIEGFSYEERDGTRDVYFTLDLKEYKRIKIPKVTPKQ"
     gene            complement(1042..3495)
                     /locus_tag="ACGI59_001974"
     CDS             complement(1042..3495)
                     /locus_tag="ACGI59_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9739760.1"
                     /translation="MGNVREEGINMYLTDNYTPKMNQIISVTDNFRRATVAVSLSTNV
                     MASSIKNSIGSASNRVNSLNSSLRKVQTTASSVSSTMTKLSSSINAVSGVIGSLNGSI
                     MILAITIAMIIDYFNKLIQKKNEFNSNIMIILIFKAKSDEVEKTKNKLLGNLKKIGGK
                     VWNIVIKAKDMTKRVISSILGKLKQVEKRPYQGSINLKDMVSSAMGRILPKLMLFKNT
                     FWSGVIAIKDMASGIISKVFPKLRLFAGKVWSGAIAVKDMASGILGSIKGKISDLTNG
                     ATIGVAVKKGVDLLGQEQNQKVVLESVMKRNTGKVNQIDVDDYYGSLVRMANDTPFDP
                     EDVVAMGTKAKMISNITGGKKEKDITQAMVDVRALNMNTSSEQDVSAAFLSAAKGNME
                     SLNTLVGENYKTFDEALEGISVKQMGLAKEMSNTIPGIISGAQTSINNGLKSIVKPFD
                     DILGQGLKKIKTFIESGLGNLAGLSEKMAGKIGNVMNGKIIIGNKYDQMQSRSVKNGK
                     EFSDSTQYRISNEAEKRKMMVENKQERFENHAATMIGNAPKAIVNAGSTLLQNIDFTA
                     LIDSLLPVVNLVNNLLDSINNKSPIAQGLISIFGTIVTTAFQLIGPVVEAVSPIITRI
                     FTFLGEYAPQINNFIETLGVIWKTVWETLGPLLETGWKIIEPILGAFFNILDKVCKIV
                     KDICKWWQTMINKIKNGSITGTVLNLVEKSKKNYKDNPYAGTKAGDSGKAYSGKKGNN
                     AFGLNYVPYNDYQTRLHEGEMVLTKQEANQYRSRKNGGNINIAKLADTIVIREEADIE
                     KITSKLVASIQLAQLGGVL"
     gene            complement(3495..3662)
                     /locus_tag="ACGI59_001975"
     CDS             complement(3495..3662)
                     /locus_tag="ACGI59_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9739761.1"
                     /translation="MYYYWKKKGIRPSLFYAMDKGELKLIEAFFALEIEEEVEKMKHG
                     YGVCPLTGGGM"
     gene            complement(3689..4135)
                     /locus_tag="ACGI59_001976"
     CDS             complement(3689..4135)
                     /locus_tag="ACGI59_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFG9739762.1"
                     /translation="MSENGLSKNINIVDLLLNSDTENLERPSTIVELKRLSTIFGQEF
                     KVMCRALTISKDEEIQNTCLKIDENMKTDIDLPEMQMLTIIEGVCDLDGKLLFKNKEL
                     MDKFKAPTPKELARKLLLPGEITNLYRILQDVMGYGKNAVIEEVKN"
     gene            complement(4261..4689)
                     /locus_tag="ACGI59_001977"
     CDS             complement(4261..4689)
                     /locus_tag="ACGI59_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFG9739763.1"
                     /translation="MANMEARNVMSGTWGELWLDGNKVAEVKKFQAKMEFTKEDIIIA
                     GQMGTDTKYIGYKGKGSITLYHVSSRMHKLIGEKIKRGSEPRFVAISKLNDPDSYGAE
                     RIAVKNIAFDDLTLADWEVGVKGEIEAPFTFTEYDFLDII"
     gene            complement(4703..5767)
                     /locus_tag="ACGI59_001978"
     CDS             complement(4703..5767)
                     /locus_tag="ACGI59_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HFG9739764.1"
                     /translation="MAIGLPSINISFKELATTVKERSARGIIAMVLKDAKALGLNEIH
                     EKEDIPVDLSAENKEYINLALMGNVNTPNKLLVYVIEGEADIQTALDFLETKEFNYLC
                     MPKAVEADKTAIKNWIIKLRDIDKVKVKAVLGKVVGNHEGIINFTTEDVLVGEKKYSV
                     DEFTSRVAGLIAGTPLSQSVTYTKLSDVVDIPKMTKVDAESKVNKGELILIKEAGAIR
                     IARGVNSLTELTAEKGEMFQKIKIVDTLDIIHSDIRKVIIDDYIGKVTNSYDNKCLLI
                     VAIKSYLEELEKSALIESDSTVEIDFEAQKSYLKSKGVDLSYMTLQEIKEANTGSKVF
                     LKAKIKVLDAMEDIDLSIEI"
     gene            complement(5772..6215)
                     /locus_tag="ACGI59_001979"
     CDS             complement(5772..6215)
                     /locus_tag="ACGI59_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6838 family protein"
                     /protein_id="HFG9739765.1"
                     /translation="MLKYKEILETIIEILKKNFTESIFIDDESVQGSEGSCFFVSILS
                     VICTPVMLNTNNKDIVISIKYLPKPQSKSIRMYEVSDELNKLFNRNIKVTDRKLNITK
                     LEQSIKKEESIYVLNFTFTLNYLDSVYEEDVVYENMEEINLNLGE"
     gene            complement(6464..6904)
                     /locus_tag="ACGI59_001980"
     CDS             complement(6464..6904)
                     /locus_tag="ACGI59_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9739766.1"
                     /translation="MENKKDILFKETDKRLHNYKYLDIKIKNINLDIKRCENEYSGCG
                     AMVYTEKTSNTYNISSSVENEVLKREERLRKLKMEKEDIEIEKEKIENALTCLNDIEM
                     EFFNLFYNSKTKNNMTYISMKLHLDRTSCYNLKKKMIFKLSEIL"
     gene            complement(7009..7206)
                     /locus_tag="ACGI59_001981"
     CDS             complement(7009..7206)
                     /locus_tag="ACGI59_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFG9739767.1"
                     /translation="MFKNNLKYYRKCKGMTQIQLARKAGITNDYISQIERGIKNPGLL
                     MAKKISSILEQNIEEVFFIQL"
     gene            7375..7695
                     /locus_tag="ACGI59_001982"
     CDS             7375..7695
                     /locus_tag="ACGI59_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFG9739768.1"
                     /translation="MFAKRLRELRKEFGLTQRELGEKVGVSQRVLGYYETENRFPDEH
                     ILNKLADVFNVSVDYLLGRTLVKENIDTVAAHRKNPHEELPEEAQEQLNDYIEFLLNK
                     YKKK"
     gene            7777..8283
                     /locus_tag="ACGI59_001983"
     CDS             7777..8283
                     /locus_tag="ACGI59_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFG9739769.1"
                     /translation="MNNLDKLFELASQEEIIIHYTTYIAGDLEGLYINKHGIKIISLL
                     SNLKQNSKKLTSILAEELGHHFTSLGYYVSSYNDYYTKIIIDKCENKALKWACEFLIT
                     EEDIINIINSGITCVYEMADILNVDITFFQKRLEFLSLKKQSLQLGNNKYLILTNLPY
                     FYIFDPIS"
     gene            8984..9739
                     /locus_tag="ACGI59_001984"
     CDS             8984..9739
                     /locus_tag="ACGI59_001984"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFG9739770.1"
                     /translation="MEKKVLATVGEKEITNIDIENALKSLDPYQAMQFKTEEGKKHLL
                     NDLVNQELFFLDAKEEKLDEEEIFKLEMKKIEENVLKQFAINKVLSSVNVTEDEKVKF
                     FEANKSSFSKPESATAKHILVDSDEKAKEILAQIKSEEISFEDAALKHSSCPSKDMGG
                     DLGTFGRGQMVPEFEEAVFSMAKGEVSEPVKTQFGYHIIKLEDLQESTESTFDEVKAE
                     VEKSLLYQKQNEVYGNKINALNAKYGNLVKYND"
     gene            complement(9882..10310)
                     /locus_tag="ACGI59_001985"
     CDS             complement(9882..10310)
                     /locus_tag="ACGI59_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HFG9739771.1"
                     /translation="MNILFFLTPKSEVAYIYEDYTIRQALEKMEYHKYSAIPIISKDG
                     KYVGTITEGDFLWTLKNDLNLDLKGLEDVPVTDINRKMDNSPVSINADIEDLVIKSLN
                     QNFIPVIDDQDTFIGIIKRRDVIGYCYEIIRGYKNLANDN"
     gene            complement(10731..10931)
                     /locus_tag="ACGI59_001986"
     CDS             complement(10731..10931)
                     /locus_tag="ACGI59_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFG9739772.1"
                     /translation="MKTGVVKWFNNEKGFGFISVEGEGDVFVHFSAITGDGYKTLEEG
                     QSVEFEVVDGAKGPQAANVVRL"
     gene            complement(11114..12268)
                     /locus_tag="ACGI59_001987"
     CDS             complement(11114..12268)
                     /locus_tag="ACGI59_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase domain-containing protein"
                     /protein_id="HFG9739773.1"
                     /translation="MKILKNKNKYIKGIMAFVFLSLATTAGVFLINDLSMKNMEEKVM
                     ESANVEGDDVKDQYKLLLENLFDYRNKAILEKNENILKGLYETDKKFGLWAYENEVKK
                     MKYLENWSSKQGVKFKDIKTRVKIKKIKEKEKDLYGIICSVSTEYKYSYENQKDKENA
                     FRIGTYHYLNVKIIDNQYVITKEWYTDPFADSLNLENIKSDDIRNYIGQQDGVELQLT
                     DEQKKAIDYAHKYCGAAADEEHGMKFNPNYRDYNPEGGDCANFASQIMFESGRFKKNS
                     IWNYSERAGTKAWVNAQAFKNYALYSGRGSLIAKGSYEEVYKEAYNLRPGDFVGYEKK
                     GRITHVSTVTGLDSRGYPLVTCHNTDRMLVPWDLGWSDKSIRFHIIKINY"
     gene            12519..13340
                     /locus_tag="ACGI59_001988"
     CDS             12519..13340
                     /locus_tag="ACGI59_001988"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine kinase"
                     /protein_id="HFG9739774.1"
                     /translation="MQKKVAAINDLSGIGKCSLSVAIPILSALKVQCCPFPTAILSSQ
                     TGYPEFTFLDFTDEMVKYSNVWKNLKVNFDSIYSGFLGSKHQIEIVANFINDYPNAFI
                     VVDPVMGDNGVMYPIFTEEMRQEIKELVKHSDLTTPNLTEACFLTGNDYTKSDYNRDE
                     LIYIAKSVSDLGPSKVVITGILEDDNILNLAYNRDNDHVFFTSVKYNNCSYSGTGDIF
                     TSILCGMLVNKHDLGVAVNTATDFIYKTINYTSQFDTDRNDGVMFENFLSDLTNI"
     gene            complement(13416..14804)
                     /locus_tag="ACGI59_001989"
     CDS             complement(13416..14804)
                     /locus_tag="ACGI59_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase"
                     /protein_id="HFG9739775.1"
                     /translation="MLGGKKSNNKKVEKLRTIFIKYLSLFFIMTISIVLLLMLSFSVL
                     LSSGVILPANYAEKQFNKYKEQIISSEKVTEDIIPSIYEYGVYTLDGNLISGTFNKKE
                     SKEVWNLMRDIEERHAYSESYIKFFKKDEVFIIKYKIVSEYSSPILRAYLPKPETLGM
                     IIFSIIFFIEIVILSKVFGKKFNIEMELLKNTTEKIEQQDLDFVVESSKIYEINNVLF
                     SMDKMKLALKDSLEKRWMLEENRKEQISALAHDIKTPLTIIHGNTDLLIEINENPELL
                     EYMEYISKGVTQIEKYINTLIDISKTENGYILNKEAISMSEFIKDVLVQIEALASTKN
                     LDVEFYKQDNLPKNITIDRELLFRAIMNIISNAIDYSPSQSKLYISVSVHNKYLKFVV
                     TDCGSGFSKADLSKATEQFYTGDLSRNSKSHYGMGLYIVNNIVQKHNGTLCIENSVKT
                     GGAMVTIEIPIV"
     gene            complement(14939..15697)
                     /locus_tag="ACGI59_001990"
     CDS             complement(14939..15697)
                     /locus_tag="ACGI59_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutG family
                     permease subunit"
                     /protein_id="HFG9739776.1"
                     /translation="MRQLIRIFCSDLIKLKRNFIILMHICIALMGMGLFLWYYKVSGA
                     DNILKIVAYLQVIAIAFPLLSSVVCSMFVEQEYYAGNYKHMLTTSNPKYLTLISKYII
                     LVCLGFVATLVSVLGFKFGISKAYFTSSFYMISILILIGCNLFEYILHLFLSLRFGKG
                     TSIGVGIVEMLLSALLLTGLGSGIWQYFPCAWGVRFITIWSSFSSSKTVEYIKVESIK
                     AYQSTGLICGFVTILAFVILCIWFSKWEGKKSEE"
     gene            complement(15699..16415)
                     /locus_tag="ACGI59_001991"
     CDS             complement(15699..16415)
                     /locus_tag="ACGI59_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HFG9739777.1"
                     /translation="MERFIRVEFQKMKGTFGMKLIWIVPVFCILLCGLGGGQNAAFNW
                     WYTTFLPGALTLISSLIVLKDEKSKYRGILPLPIEMKDVWTGKMIYCIICCFIASIIS
                     AIGVVFVGIIYKETISIGQNIIASILIILLFMWQIPLCMFLASKFGMFVTVLTNMFFT
                     IVGVAVFSTTEMWYMFPYSIPSRLMAVVLHILPNGLPVPEGSEFLNNAVILPGVLISI
                     VLFVLFALATRVWFNNREVK"
     gene            complement(16417..17124)
                     /locus_tag="ACGI59_001992"
     CDS             complement(16417..17124)
                     /locus_tag="ACGI59_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic protection ABC transporter
                     ATP-binding protein"
                     /protein_id="HFG9739778.1"
                     /translation="MSNYILQVKGVSKKFKEQMAVDNISLAIKSNSIYGLLGPNGAGK
                     STLLKMITGILNPTSGKIMFEGHRWTRKDLSNIGSLIESPAIYENLTARENLKVHTTL
                     LGLPDSRIEEVLETVDLRDTFKKRSGQFSLGMKQRLGIAIALLNHPKLLILDEPTNGL
                     DPIGIEELRELIRSFPKQGITVILSSHILSEVEQVVDEIGIISNGVMGYQGEVSKEQN
                     LEELFMKVVAENRKRGQ"
     gene            complement(17240..17776)
                     /locus_tag="ACGI59_001993"
     CDS             complement(17240..17776)
                     /locus_tag="ACGI59_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFG9739779.1"
                     /translation="MNKIAVSFLIIATTLLSTACMDYSISAVELVDSKESAVVKKDED
                     AKEETTSKMINSKKTTKIPIEIISKDEKIVKYLQIDEESSLKDKLRLILDTLSNEYFN
                     GLDMEVEVKEKDNLVKINLIEPDKKSRVSWKDDYLNEQNIIYTINNIIKNVIQEEDNS
                     IWIEEVEIYYNGKLIELR"
     gene            complement(17878..18948)
                     /locus_tag="ACGI59_001994"
     CDS             complement(17878..18948)
                     /locus_tag="ACGI59_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HFG9739780.1"
                     /translation="MNKKLAIFAAVLLVIGIIGTTWSGILVMPSLINFGLEKEAELNK
                     ENKLYQEKVNIDKLDIAVDNINVMIRKSSNEDVRVTTRGNNEFYKYSVTLQDKTLVVK
                     GERKSENKIKKIRSFDHLLNTSINSMFSRDYREIRIYVPNNVDINASSISSYLFISDD
                     VDSNNITYKTSSGGFSRAITENKVNRLENLNLISNSNLHLSTKSILGVKNVNIESESL
                     DISSENGDVFINNIEEYIPENVNIKEKIGRNSNYESEFYLYSDMPIAKFLDIEVPNSK
                     VRLDIPVNKYKFNCDIKSKEAIEEFNNDEEYDNDSYDYEHNEEHSNKYRNTREIKGLL
                     NKNLSNLEKEYTIKINSNSLEL"
     gene            complement(18941..19999)
                     /locus_tag="ACGI59_001995"
     CDS             complement(18941..19999)
                     /locus_tag="ACGI59_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HFG9739781.1"
                     /translation="MNLNKTEFLEILKDYLKKDFSESEVNDILRDYEEYFVDGLIEGK
                     SDMEIIASLGSPKSIAKELILQTKEKNIEEEKIINTDVLNDKFSKFKSDMKKGYSILK
                     DEAKEKYSKSKENIDGKIMNTDILNGKFSRFKSNVKKDYSILKDEAKEKYNKSKENIN
                     DKLTPSIEEGVGGLSRQVIKGLLVVLSLFLIVPAFAFVCAIVSATIALVCSLIAFVIL
                     IPFVIKFIAMVPDVMMFCIFATIAFVGLEILAWQILLFAIKLGKQLLNNYIHWIKRKN
                     IYIKANEKMEKRKKATLNDEDLNKDINENSYNNDYYNREEESIKETDEYNDDSINFEA
                     IYKDLEDIENKKGEDKHE"
     gene            complement(19986..20318)
                     /locus_tag="ACGI59_001996"
     CDS             complement(19986..20318)
                     /locus_tag="ACGI59_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFG9739782.1"
                     /translation="MNTQFRKGVLEICVLALISKKDMYGYEIVHNISKVIDVNEGTIY
                     PILRRLTKDLYFETYILESNEGPARKYYRITSLGKENLSNLMEEWREFVKAVEILISE
                     DEGGNELE"
     gene            20517..21638
                     /locus_tag="ACGI59_001997"
     CDS             20517..21638
                     /locus_tag="ACGI59_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFG9739783.1"
                     /translation="MKDDKTILILTAQFGAGHISAAKAVKECIIEKYSNYNVVIQNFI
                     NASIPMMNKPMVKLYENNTKYTPGLYNYYYYFKKSFDSRHDLSHKLYTPKLSEYIADI
                     NPDLIISTFPLAAACVNNFKIKNPDINIPTLTVITDVVDSMEWVFENTDLYFVPSPEI
                     KNRFFQKGINPDSIKVTGVPVDKRFQIESKELCCDKYRLLLLGGGRGLFDIDEDFMHW
                     IDEFIEEHSNSIEITIVTGKNKKLYDNLTHKKPLKNIKVLGFVNDMYNLIRECDLMLT
                     KPGGATIFEAIQSQTPVLVKMPKVGQEIENAKFIIDKGLGMIYSDDLDLKNIFYKLVS
                     NEFDSIINFMKKNLKEFKTVIHPEKIADYISELIDNHYS"
     gene            complement(21766..23664)
                     /locus_tag="ACGI59_001998"
     CDS             complement(21766..23664)
                     /locus_tag="ACGI59_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HFG9739784.1"
                     /translation="MDSLLYVIEKDKHTNEELREILKSNKNIRFVSLMGVDLGGNATD
                     EKIPVELFLEDIDKFLESAIQTDGSSVELYNIATLNNAKVDLMPDKSCHWYVDYNMEY
                     IDEEVGLPVGTLKIPAFLIHDNKKVCSRGVLQKADKYFKKSMYEIFREYPHVINNIGI
                     DSVDDIEEIMLTAATELEFWVNTPEDKADLEKLYVSQSLKEQYWKRTHGIIRTCLEKS
                     LIILQKLGVNPEMAHKEVGGIQSSISIDGRTNHAMEQLEVSWKFSTPLQAADNELLVR
                     DVIEDVFTSHGLEVTFKAKPIHGVAGSGGHTHVGVSAKLKDGSIKNLFAPKDLKEDYL
                     SELGYGALMGLLYNYEVLNPIVTASNDGFNRLVPGFEAPVCIVTSLGHSYEIPSRNRS
                     VLVGLIRDMKNPKTVRFELRSPSPLSNTYLVIAGCYQTMLDGIKAAAKSGLSTKELEK
                     ELSKNVGEESFYLEKDRAYRDENDVFEHYSLEERNARFGIPPATVYENMKNLEIYASK
                     LKSLKQGDVFTDSIIESFKIGAIDKWQKKLKTRIIEAGIQKIRSIVKIHTKENMDALD
                     EVVWNSISDLKFNIMKDTLTRESLFTRVREAIENKDYQTASDLQIELKRSMEEIQQLY
                     MQYKKNIY"
BASE COUNT         7810 a         4036 c         2596 g         9500 t
ORIGIN      
        1 acatttaatg taacagttcc tttttcaata acattaaact tatctaaatt ggcctctatc
       61 atatactttt tctttgtctt tttacttgct tctgtatatg cactcattat tgtgtcataa
      121 ccatttactc ctataaacat ctttgtatac ttgacattgg tctttgctat tgttccaact
      181 gaaagcctat tttcagcaaa tacttgcttt gctatgtctt caactaactt atctttaaaa
      241 ttgtatgaca cttcactttg tgtaagtaaa aatcccatat ctttagatac aaaactaatt
      301 tcattactgc ttgaatcttt agacctatta attatcattc ctctaaagag ttctttctca
      361 tctacataga aacagactgt actagctatt ggtatatcta tttgttggaa atttacatca
      421 aatgatgatt gtattattga aaactctagt gtccttgatg gagatttata atcacctgac
      481 catgatacct tgtctactat gtcagttatg tcatatatac ttccgttttt tatgtgtacc
      541 caaatcttaa taataaaaaa tcacctgcct tttcatttaa ttctataagc agttatttat
      601 aacttattat ctataatagt tattgttttg gagttacttt tggtatcttt attcttttat
      661 actcttttaa atctaatgta aaatatacat ctcgagtccc atctctttct tcataactaa
      721 acccttcaat tatgacttcc atatttatat ttgtttccgt aattgtaaat cttaatataa
      781 aaccttcctt catccatttt tctatcttat ttacacaatc atatggtgat ggaaaacctg
      841 tatagtcaca gaaagtatag tctttatttg gaaaaaaact tgatattgaa atgcttttaa
      901 gagctacacc accaaaagtt gctatttcac ctattttgag tatactagaa gagtttacag
      961 cagcttttcc attaatacta aaggaagatg gaaatactgg aaatctaaat ctatcttcag
     1021 cttgtctaag ccacatttcc attataagac accccctaac tgtgccaatt ggatacttgc
     1081 aactaatttt gatgttatct tttctatatc agcttcttct ctaatcacta ttgtatcagc
     1141 taacttagct atgtttatat ttccaccatt ttttctgctt ctatattgat ttgcttcttg
     1201 tttagttaaa accatttcac cttcatggag tctggtttga tagtcattat aaggaacata
     1261 gttcaatcca aatgcattat tacctttctt acctgaataa gctttaccag aatcaccagc
     1321 ctttgttcca gcatatggat tatctttgta atttttttta ctcttttcca ctagatttaa
     1381 aactgttcct gtgatgcttc cattttttat cttattaatc atagtttgcc accacttgca
     1441 tatatcttta actattttac atactttatc taatatgtta aaaaaagctc ccaatattgg
     1501 ctctataatt ttccatccag tttccaacag aggtcctaag gtctcccata cagttttcca
     1561 aataacaccc agtgtctcta taaaattgtt tatttgaggt gcatattcac ctaaaaaagt
     1621 aaaaattctt gtgataatag gactaacagc ttcaactaca ggtccgatta gttggaatgc
     1681 tgtagttact attgtaccaa atatacttat taatccttgt gcaattggtg atttattgtt
     1741 tatactatct agtaaattat ttactaagtt tactactgga agtaatgaat ctattaatgc
     1801 tgtaaaatcg atattttgta atagtgtact tcctgcgtta acaattgctt ttggtgcatt
     1861 ccctatcatt gttgctgcat gattttcaaa acgttcttgc ttattttcaa ccatcatttt
     1921 acgcttttca gcctcattag aaattcgata ttgagtagaa tcagaaaact cttttccatt
     1981 ttttacactt ctagattgca tctggtcata tttgttgcca ataattatct taccattcat
     2041 tacattgcct attttaccag ccattttttc agataagcca gctaaattgc ctaatccact
     2101 ttctataaaa gtttttattt tctttagtcc ttgacctaaa atatcatcaa aaggtttaac
     2161 aatactcttc aagccattgt taatgcttgt ttgagctcct gatattatac ctggtattgt
     2221 attactcatt tctttagcta accccatctg ctttacactt atgccttcca atgcttcatc
     2281 aaaagtttta taattttctc ctaccagagt attaagagat tccatgtttc cttttgctgc
     2341 acttaagaaa gctgctgata catcttgttc actacttgta ttcatattta aagctctaac
     2401 atctaccata gcttgagtta tatctttttc ttttttgcca ccagtaatat tactaatcat
     2461 tttagcttta gttcccattg caacaacatc ttcagggtca aaaggcgtat catttgccat
     2521 tcttactaaa ctgccataat aatcatcaac atctatttga ttaacttttc cagtatttct
     2581 tttcattaca ctttctagaa caactttctg attttgttcc tgaccaagta aatcaacacc
     2641 ctttttcaca gcgacaccta tagtagcacc atttgtcaaa tcagatatct tcccttttat
     2701 cgaaccaagt attccacttg ccatatcctt tacagctatt gcaccactcc ataccttacc
     2761 tgcaaataat ctcaatttgg gaaatacttt acttataatg ccacttgcca tatcttttat
     2821 agctattaca ccactccaaa aagtattttt aaacaacatt aacttaggca aaattctacc
     2881 catagcacta ctcaccatat ctttaagatt aatacttcct tgataaggac gtttctctac
     2941 ttgttttaat tttcccaaga tactacttat aactctctta gtcatatctt ttgcttttat
     3001 tacgatattc caaaccttgc caccaatctt ttttaaattt ccaagtaatt tatttttagt
     3061 tttttctact tcatcacttt tagctttaaa tattaatata atcataatat ttgaattaaa
     3121 ctcatttttc ttttgaatca acttattaaa ataatcaata atcatagcta tagttattgc
     3181 tagtatcata atacttccat ttaaacttcc aataactcct gaaacagcat ttatgctaga
     3241 acttaatttt gtcatagttg aacttacact actagcagta gtttgaactt ttcttaacga
     3301 ggaatttaaa ctgtttactc tattacttgc acttccaata gaatttttta tgctactagc
     3361 cattacatta gtggaaagtg aaacagccac agttgctctc ctaaaattat cagttactga
     3421 tataatttga ttcatttttg gtgtgtaatt atctgtaagg tacatattta taccttcttc
     3481 tctcacattt cccattacat accacctcct gtcaaaggac acactccata tccatgtttc
     3541 attttttcaa cttcttcctc aatttctaag gcgaaaaaag cttcaataag ctttaattcg
     3601 cctttatcca ttgcataaaa aagggacggt cttatacctt ttttcttcca ataatagtac
     3661 attattgtag tcctggtatc cgtccctatt agttttttac ctcttctatc actgcatttt
     3721 taccataacc cataacatct tgaagtattc tatataagtt ggtaatttca cctggtaata
     3781 ataattttct tgctaattct tttggtgttg gagccttaaa tttatccatt agctccttat
     3841 ttttaaataa aagctttcca tccaaatcac aaacaccttc tataattgta agcatctgca
     3901 tctccggtaa gtctatatcc gttttcatat tttcatcaat tttaagacaa gtattttgta
     3961 tttcttcatc tttacttatt gttaaagctc tacacattac tttaaattcc tgcccaaata
     4021 tagttgataa tctcttaagt tcaactatag tactcggtct ttctaagttt tctgtatctg
     4081 aatttaaaag taaatctact atgtttatat tttttgataa tccattttca ctcattatta
     4141 tttgctcctt tatcaatcaa attcaatcaa ctaacttttt tacttattaa ctatctgaat
     4201 ttattaagta tatatttact aaatatcagt ataaaaccaa atataaaact aaatataaaa
     4261 ctaaattata tcaagaaaat catactcagt aaatgtgaaa ggtgcttcta tctctccttt
     4321 tacgccaacc tcccaatcag ctaaagttaa atcatcaaat gctatattct ttactgctat
     4381 tctttctgct ccataagaat ctgggtcatt taatttagat atagcaacaa acctaggttc
     4441 agaacctctc tttatctttt ctccaattaa cttgtgcatt cttgaactaa cgtggtatag
     4501 agttattgag ccttttcctt tatatcctat atactttgta tcagtaccca tttgacctgc
     4561 tattataata tcttcttttg taaattccat ctttgcttga aatttcttta cttctgctac
     4621 tttgtttcca tcaagccaaa gttctcccca agtaccactc attacatttc tagcttccat
     4681 atttgccata ttaataatcc tcctatattt ctattgataa atctatatct tccatagcat
     4741 caagtacttt tatttttgct tttaaaaata ctttagaacc tgtgttagct tcttttattt
     4801 cttgtaatgt catataagat aaatctactc cttttgattt taaatacgat ttttgtgctt
     4861 caaaatctat ttcaacagta gaatcagatt ctataagtgc tgatttttct aattcttcta
     4921 aataactttt tatagctact atcaataaac atttgttgtc ataactgtta gtaacctttc
     4981 ctatatagtc atctattatc acctttctta tgtcactatg tataatatct aaagtgtcaa
     5041 ctatttttat tttctggaac atttctcctt tttctgctgt taattcagtt aaagaattta
     5101 ctcctctagc aattcttata gctcctgctt ctttaataag tataagctct cctttattaa
     5161 cctttgattc tgcatcaact ttcgtcatct taggtatatc gactacatca ctaagtttag
     5221 tataagttac tgattgactt aaaggtgtac ctgctataag tccagccacc ctacttgtaa
     5281 actcatcaac actgtatttc ttttctccaa ctaacacatc ttctgtagta aaattaatta
     5341 tcccttcatg atttcctaca acttttccta atacagcttt aaccttaacc ttatctatat
     5401 ctctaagttt aattatccaa ttttttatag cagtcttatc agcttctact gcttttggca
     5461 tacatagata attaaattcc ttagtctcta aaaaatctaa tgcagtttga atatctgctt
     5521 ctccttctat tacataaact aataacttat ttggagtgtt aacatttccc atcaaagcta
     5581 aatttatata ttctttattt tcagcagata aatcaactgg tatatcctct ttttcatgta
     5641 tttcattaag acctagtgcc ttagcatctt taagtaccat tgcaattatt cctctagctg
     5701 aacgttcttt aacagttgta gctagctcct taaatgatat gttgatactt ggtaatccta
     5761 tagccatact atcactctcc taaatttaaa ttgatttctt ccatattttc atatactaca
     5821 tcttcttcat atacactatc cagatagttt agtgtaaatg taaagttcaa tacataaatt
     5881 gactcttctt ttttaatact ttgttctagc tttgttatat ttaattttct gtctgttacc
     5941 tttatatttc tgttgaataa cttatttaat tcatctgaaa cttcatacat tctaatactc
     6001 tttgactgtg gttttggtaa gtattttata gagataacaa tatctttgtt attcgtattt
     6061 aacattacag gtgtacaaat aactgatagt atacttacaa aaaaacaaga cccttcagag
     6121 ccttgcacac tttcatcatc aataaaaata ctttcagtaa agtttttttt gagaatctca
     6181 ataattgttt ctaatatttc cttgtattta agcaaataat caccacacta tcactaaata
     6241 ttcttgctta atctgataat cttactataa atgatataag tcatttttaa atatatatct
     6301 catttattcc tgtcaagtat cttctatatt tttaggtaat attattcttt tcaatctatc
     6361 acctccttca atatggattt ctttcacaat aacattatct cacatatttt attgcctatt
     6421 gttgtaatgg tgtatataaa gtgttgtaaa attgtcctat tttttataat atctcactca
     6481 atttaaatat catctttttc tttaaattgt agcaagatgt tctatctaag tgtagtttca
     6541 tagaaatata tgtcatattg ttttttgtct tactattata aaaaagatta aaaaattcca
     6601 tttctatatc atttagacac gttagagcat tttctatctt ctctttttct atttctatat
     6661 cttctttttc catttttaat tttcttaatc tttcctctct ttttaacacc tcattttcca
     6721 cagaagagct tatgttatat gtgttactag tcttttctgt atataccatt gctccacatc
     6781 cagagtattc attctcacat ctttttatgt ccaagttaat attctttatc tttatatcca
     6841 aatacttata attatgtaat cttttatctg tttctttaaa taatatatct tttttgtttt
     6901 ccatagtact tccccttttt tatatactta ttattagctt caaatttaca ataatctctt
     6961 tagtgcacag ttttttacta atctcacaac cttcaagaac atattgttct ataactgtat
     7021 aaaaaaaact tcttctatat tttgttctaa aatactagaa atcttcttag ccataagaag
     7081 tccaggattt tttatgcctc tttctatttg agatatataa tcatttgtaa ttccagcctt
     7141 tctggcaagt tgaatttgtg tcataccttt gcattttcta taatatttca agttattttt
     7201 aaacattgtt ttatacctcc attactatta agaatatttt gttcttcctc acttagatta
     7261 tatagaatta tttgttcttt atcaatagat tttagaaaaa aatattcttt tatattttat
     7321 tagttttatt ttttagaaca atttgttata atctatacat aaggtggtgt tataatgttt
     7381 gccaaaagat taagagaact tagaaaggaa tttggattga ctcaaagaga acttggagaa
     7441 aaagtaggcg tttcccaaag agtcttaggg tactatgaaa cagaaaacag atttcctgat
     7501 gaacacattt taaataaatt ggctgatgta tttaatgtat cagttgatta ccttcttgga
     7561 agaaccttag ttaaggaaaa tattgataca gtagctgcac atagaaaaaa tccacatgaa
     7621 gagttacctg aagaagcaca agaacaactt aatgattata tagaattttt actaaataaa
     7681 tataaaaaaa aataaaccat atgatgagca gtctattttt ctgctctttt aatataattc
     7741 aaaggcaaac atacattcta ttttaggggg atttttatga ataatttgga taagcttttt
     7801 gaattagctt ctcaagaaga gataataatt cattatacca cttatattgc aggtgattta
     7861 gaaggactat atataaacaa acatggtatt aaaattatat cattactcag taatttaaaa
     7921 caaaactcaa aaaaattgac atccatctta gctgaagaac ttggacatca tttcactagc
     7981 cttggatact atgtatcctc ttataacgat tattatacaa aaattattat agataagtgc
     8041 gaaaataaag ccttaaaatg ggcttgtgag ttcttaatta ctgaagaaga tataataaac
     8101 ataattaact ctggcatcac ttgcgtttat gaaatggcag acatacttaa tgtggatatt
     8161 actttctttc aaaaaagatt agaatttcta tcattaaaaa aacagtcttt acaacttgga
     8221 aataataaat atttaatatt aactaatttg ccgtatttct acatatttga tccaatttca
     8281 taataaaaat agtagtaatt ttaactggct taacagtaaa atctatatat ttattttaac
     8341 aaattttggt ttctataaat tcaatattaa tatataataa tgatatcata ttatttaaaa
     8401 ttctaaaaac aaatatatgc ttttgttaag aaaggttgtt tgatgaatgt ataaagaaaa
     8461 gttgtttgat aattatttta agttcttagc actattattt tggccaatta tgtggtataa
     8521 gtggatagta atttcaaatg gtactcttga aaatatgtta tttaccatat atgcaatcgt
     8581 cgcaattgtt ttcatcattt tatattcggt gtttatgata aaatataaag acattacaca
     8641 aatagatttc ttttacagga taagtactct acttgctttt atatttacac tttttagctt
     8701 tttaatatac ccaaagagtt tatttttctt gtatttaaag attattttta ctggaatata
     8761 cctttattat tcaatagtaa aaactcttaa atttaaagat gacgaaggtg tagtgggtat
     8821 tatgagctca cttttattaa ttgtaataac tttgttctac taaatttaat tctatttgac
     8881 ttaatatggc tttaaatatt agacatctac attattttct gatattattt aagatggtag
     8941 tcttatatta ataatgaaat acactatgaa ggagttaact attatggaaa aaaaagtttt
     9001 agcaacagtt ggagaaaaag agataactaa tattgatatt gaaaatgcat taaaatcatt
     9061 agacccttat caagctatgc aatttaaaac tgaagaagga aaaaaacact tattaaatga
     9121 tttagtgaat caagaattat tcttcttaga tgctaaagaa gaaaaattag atgaagaaga
     9181 aatatttaaa ttagaaatga aaaagataga agaaaatgta ttaaagcaat ttgctataaa
     9241 taaagtttta tctagtgtta atgtaacaga agatgaaaaa gtaaaattct ttgaagctaa
     9301 taagagctct ttctctaaac cagaaagtgc aactgctaaa catatattag tagatagtga
     9361 tgaaaaagct aaagaaatac ttgctcaaat aaaatcagaa gaaatatctt ttgaagatgc
     9421 agctcttaaa cattcttctt gcccatctaa agatatgggt ggtgatttag gaacattcgg
     9481 tagaggacaa atggttcctg aatttgaaga agctgtgttc tctatggcta aaggtgaagt
     9541 aagtgaacca gttaaaactc aatttggata ccatataata aaattagaag accttcaaga
     9601 aagtacagag tctacatttg atgaagttaa ggctgaagtt gaaaaaagtt tattatatca
     9661 aaaacaaaat gaagtttatg gaaataagat aaatgctctt aatgctaagt acggaaacct
     9721 tgtaaaatac aacgattaat cataaggtca tatataaaaa agctgtcttt tcacaatatt
     9781 tattagaaaa gacagctttt tatattaaag tttaaatatt cagtttcata ttatcatttt
     9841 tttattgtaa agaactcagc ttaaaagttt aaataagatt tttaattatc attagctaaa
     9901 tttttatatc cacgtattat ctcataacag taacctatta catctcttct ttttattatc
     9961 ccaataaatg tgtcttggtc atctataaca ggaatgaaat tttgatttaa agattttata
    10021 actaaatctt caatatctgc gttgatggaa acaggagaat tatccatttt tctatttata
    10081 tctgtcactg gaacatcttc caatcccttt aagtccagat ttaaatcatt ttttaatgtc
    10141 cataaaaaat caccttctgt tatagttcca acatatttac catccttact tattattgga
    10201 attgcagagt atttgtgata ttccattttt tctagggctt gccttatagt gtagtcttca
    10261 tatatatatg ctacttcact ttttggtgtt agaaagaata atatgttcat tacactaccc
    10321 accgttactg aaaacttata aatattactc aatatttata atgttacttt tttgcagtaa
    10381 ctttctcctt tcttattttc taagtattct taatactata cgctgtaaca agcgtttttc
    10441 ctttgctcat tttaatctta ttatgcttta ttaatatttt tatatggtct ataagtatca
    10501 cttattctct ttttagaaaa tttactaaaa attaattgac ttaaacttag ccataataat
    10561 tttactctac actattaatt ttaacatatt tatctcatta ttaatgattt tattttaata
    10621 attttaattt attttgctat taattttatt tacaaaaact gaaaaaaccg ctaaatgatt
    10681 agcggcttat ctctcatatt actagaacac taatatatat gaatttttaa ttataatcta
    10741 actacgttag cagcttgagg acctttagct ccatctacta cttcgaactc aacgctttgt
    10801 ccttcttcta atgttttgta tccatctcca gttatagctg agaaatgtac gaaaacatct
    10861 ccttcacctt ctacagatat gaatccaaat cctttttcgt tgttaaacca tttaactaca
    10921 ccagttttca ttaataaatc ctcctaaaaa aacacaaata tttagctact cttttctgta
    10981 accttattac tactctatca tatatagcta tgtttgtcaa ttattattat ttttatacat
    11041 taataatcaa tttttttact ttataatttt aaatgattat tatcttttac actttatttt
    11101 tctttataat tttttaataa tttattttta ttatatggaa tcttatggac ttatcactcc
    11161 agcctaaatc ccatggcacc aacattctat cagtattatg gcaagttacc aaaggatatc
    11221 ctcttgagtc taatcctgtc actgtcgaaa catgtgttat tctgcctttc ttctcataac
    11281 caacaaaatc ccctggacgt aaattataag cttctttgta tacttcttca taggaacctt
    11341 tagcaattaa tgaacctctt ccactataca gtgcataatt tttaaaagct tgtgcattta
    11401 cccatgcttt tgtacctgct ctctcgctat aattccaaat tgagttcttt ttgaaccttc
    11461 cactttcaaa cattatctgt gatgcaaagt ttgcacaatc tccaccttct ggattataat
    11521 ccctatagtt tggattaaat ttcataccat gttcttcatc agctgcggct ccacagtatt
    11581 tatgtgcata atctatagct tttttttgtt catctgtaag ctgtaactct acaccatctt
    11641 gttgaccaat ataatttctt atatcatcag attttatatt ttctaaattt aaagagtctg
    11701 caaaagggtc tgtataccac tctttagtta tgacatattg attatctata atttttacat
    11761 ttagataatg atatgtacct attctaaagg cattttcttt gtccttttga ttttcatatg
    11821 aatacttata ttcagtggat actgaacaga ttataccata gaggtctttt tctttttcct
    11881 ttattttttt aatcttaacc cttgtcttta tgtctttaaa ttttacacct tgtttactag
    11941 accagttttc taaatatttc atctttttta cttcattttc ataagcccac agaccaaatt
    12001 ttttatccgt ttcataaaga ccttttaaaa tattttcatt tttctctaat attgccttat
    12061 ttctgtagtc aaataagttt tcaagcaata gtttatattg gtcttttaca tcatcacctt
    12121 caacatttgc agattccatg actttttctt ccatattttt catacttaaa tcattgatta
    12181 aaaatacacc tgctgtagta gctaaagaca aaaatacaaa tgccattata cctttgatgt
    12241 acttattctt atttttcagt attttcaaag ctacacctcc caaatagtac accttacaaa
    12301 tcaaattaca aagaatattc tgtcacatat agtaattttc atatttaata attctctatt
    12361 ttattatgtc caatattaaa aattaattgt atcaatacac tgtttttttt acaatttttt
    12421 attttataat ctactttttt ctttttttta tagtaatata tatgtatata aatattttgg
    12481 tatcattata attaatacat actaatggag ggttttttat gcaaaaaaaa gtcgcagcaa
    12541 ttaatgactt atctggaata ggaaaatgct cattatcggt tgctatacct atactttcag
    12601 cattaaaggt tcaatgttgt ccatttccaa cggccatact ttcaagtcaa actggttatc
    12661 ctgaatttac tttcttagac tttactgatg aaatggtaaa atattcaaat gtttggaaaa
    12721 atttaaaagt caattttgat agtatatata gtggtttttt gggttcaaag catcaaattg
    12781 agatagtggc taattttata aacgattatc ccaatgcttt tatcgtagtt gaccctgtga
    12841 tgggtgataa tggagttatg tatccaatat tcacagaaga aatgagacag gaaattaaag
    12901 aattagttaa acattcagat ttaactactc ctaatttaac agaagcttgt ttcttaactg
    12961 gaaatgatta taccaaaagc gattataata gagatgagct tatatatatt gcaaaatctg
    13021 tatctgattt gggacctagc aaagtcgtta ttactggtat attagaagat gataatatat
    13081 taaatttggc ttacaataga gataatgacc atgtattctt tacatcagtc aaatataata
    13141 attgttctta cagtggaact ggagatatat tcacatctat actttgtggt atgttagtaa
    13201 ataaacatga tttgggagta gcggttaata ctgcaacaga ttttatatat aaaactataa
    13261 attacacatc tcaatttgat actgatagaa atgatggagt tatgtttgaa aactttttat
    13321 cagatttaac caatatataa aatcaaaaaa actatccatt ttataaatac ctataaatca
    13381 taaaatttag gtatttacaa aatggatatt atttactaaa ctattggtat ttcaattgtt
    13441 accatagcac caccagtttt cacagaattt tcaatacata aagtcccatt atgtttttgt
    13501 acaatattat ttacaatata gagtcccatt ccataatgag atttagaatt tctactcaaa
    13561 tctcctgtat aaaactgttc tgtagctttt gacaaatctg ctttagaaaa accacttcca
    13621 caatctgtaa ctacaaattt taagtattta ttatgtactg atacacttat gtatagttta
    13681 ctctggcttg gtgaataatc tatagcattt gaaattatat tcataatagc tctgaataac
    13741 agttctctat ctatagttat atttttaggc aaattatctt gtttataaaa ctccacatct
    13801 aagtttttag tactagctaa tgcttctatt tgcactaaaa catcttttat aaactcactc
    13861 atactgattg cttctttatt taatatatag ccattctctg tttttgatat atctattaaa
    13921 gtattaatat atttttctat ttgagtgaca ccctttgaga tatattccat gtattctaat
    13981 aactcaggat tttcatttat ttcaattaat aagtcagtat ttccatgtat aattgttagt
    14041 ggtgttttta tatcatgtgc cagtgcagat atctgttcct ttctattttc ttctagcatc
    14101 catcgttttt ctaaagaatc ttttaacgcc aacttcattt tatccataga aaaaaggaca
    14161 ttattaattt cataaatttt actagattct acaacaaaat ctaaatcctg ttgttctatt
    14221 ttttctgtag tattttttaa aagctccatt tcaatattaa atttcttgcc aaatactttt
    14281 gataatataa caatttcaat aaaaaatatt atactaaaaa taatcatacc caatgtctct
    14341 ggttttggta aatatgctct taaaattgga gaactatact ctgatactat tttgtatttt
    14401 attataaaaa cttcatcttt cttaaaaaac tttatataac tttctgaata tgcatgcctt
    14461 tcttcaatat ctctcattaa attccatact tcttttgatt cttttttatt aaaggtccct
    14521 gaaattaaat tcccatccaa agtataaacc ccatactcat atatacttgg aattatatct
    14581 tcagttactt tttcacttga aataatctgt tctttatatt tattaaattg tttttctgca
    14641 taattagctg gtaaaattac acctgatgat aataatacag aaaatgataa cattaaaagg
    14701 agtacaattg aaattgtcat aataaagaat aaagataaat attttataaa aatagttctt
    14761 aacttttcaa cttttttgtt gttagatttt ttacctccaa gcatttgtac cctgtcctcc
    14821 aatagtttaa ttaattcata attaataccc aaccagtttt ttatttgatt attttatatc
    14881 tttttattgc taagttatat tttaacataa taatataaat aaacagtcaa tttttactct
    14941 attcttctga ttttttacct tcccatttag aaaaccaaat acataaaatt acaaatgcta
    15001 aaattgttac aaaaccacaa attaaacctg ttgattgata tgcttttatt gattcaacct
    15061 ttatatattc aaccgtttta gaacttgaaa aagacgacca aatagttata aatctaacac
    15121 cccatgcaca aggaaaatac tgccaaatac cagaacccaa tccagttaat aataatgctg
    15181 aaagtaacat ttcaactata cctactccaa ttgatgttcc ttttccgaac cttaaactta
    15241 agaataaatg taatatgtat tcaaataaat tacacccaat taaaattaga attgaaatca
    15301 tataaaagct tgatgtaaag taagccttag atataccaaa tttaaaacct aatacagaca
    15361 ctaatgttgc tacaaaacca agacatacta agattatata tttacttata agagtaagat
    15421 attttggatt agatgttgtt aacatatgtt tataattccc tgcataatat tcttgttcaa
    15481 caaacattga acacacaaca cttgacaata aaggaaaagc tattgctata acttgtaaat
    15541 atgctactat ctttagtata ttatcagctc ctgaaacctt ataataccat aaaaacaatc
    15601 ccatccccat taaagcaatg caaatgtgca ttagtattat aaaatttctc tttaatttta
    15661 ttaaatcaga acaaaatatt cttattaact gtctcatatt acttcacctc tctattattg
    15721 aaccaaaccc ttgtagctaa agcaaataat acaaataaaa caatagatat taatactcct
    15781 ggcaaaataa cagcattatt taaaaattca cttccctctg gtacaggaag accatttggc
    15841 agaatatgca atacaacagc cattaatctt gaaggaatcg aatatggaaa catataccac
    15901 atttctgtag ttgagaaaac agcaactcca acaatagtaa agaacatatt tgttagaact
    15961 gtaacaaaca taccaaattt tgaagctaaa aacatacaaa gaggaatctg ccacataaat
    16021 agtaaaataa ttaatatact tgcaatgata ttttgaccta ttgaaatagt ttctttatag
    16081 attatcccaa caaaaactac acctattgca gaaataattg atgctataaa acaacaaata
    16141 atacagtaaa tcatttttcc agtccaaacg tctttcattt caattggcaa tggtaaaata
    16201 ccacgatatt tacttttttc atcttttaat actattaaag aagatattaa tgttaatgct
    16261 cctggaagaa atgtcgtata ccaccagttg aaagcagcat tttgccctcc acctagtcca
    16321 cacaaaagta tacaaaacac tggaactatc catatcaact tcattccaaa agttcctttc
    16381 atcttttgaa attccactct aataaatctt tccatattac tgccctctct ttctattttc
    16441 agctacaact ttcataaaaa gttcttctaa gttttgttct tttgatactt ctccttgata
    16501 acccattact ccattagaaa ttattcctat ttcatctaca acttgttcaa cttcagacaa
    16561 gatatggctt gataaaataa ctgtaattcc ttgttttgga aaagaacgaa tcaattctct
    16621 aagttcttca ataccaatag ggtcaagacc atttgtaggc tcatctaaga ttaaaagttt
    16681 tgggtgattt aataatgcaa tagcaatacc aaggcgttgc ttcatgccta gagagaattg
    16741 acctgaacgc tttttaaagg tatctcttaa atctacagtc tctaaaactt cttcaatgcg
    16801 agagtcagga agaccaagta aagtagtatg gacttttaaa ttttctcgtg ctgttaaatt
    16861 ctcatatata gcaggagact caataagaga accaatattt gataaatcct ttctagtcca
    16921 tctatgtccc tcaaacatta tttttcctga agttggattt aaaatcccag ttatcatttt
    16981 taataaagta gatttcccag ctccatttgg tcctaataat ccatatatag aattagattt
    17041 aattgctagt gaaatgttat caactgccat ctgctcctta aattttttag aaactccttt
    17101 tacctgcaaa atataattac tcataatttt catcctttct tttgtcaaac ttatttacaa
    17161 gaatcattct aaatcataaa tataaggatt ttataagaat taaaaaacaa aaaaagatgt
    17221 gttaaaaaca catctttttt tatcttaatt ctattaattt tccattgtag tatatctcta
    17281 cttcttcaat ccaaatgcta ttatcttctt cttggatgac atttttaata atattattta
    17341 tagtatatat tatattttgt tcattcaaat aatcatcttt ccaagaaact ctactttttt
    17401 tatctggttc tatcaagttt attttaacaa gattatcttt ttccttaact tctacttcca
    17461 tgtctaaacc attaaaatac tcatttgata atgtatctaa gattaatcgc agtttatcct
    17521 taagtgaact ttcttcatca atctgtaagt actttactat tttctcatcc tttgaaatta
    17581 tctctatagg tattttagta gtttttttag aattaatcat tttactagtt gtttcttctt
    17641 ttgcatcttc atccttcttt acaactgctg attctttaga atcaactagc tcaactgcac
    17701 tgatgctata atccatacat gccgtactca ataaagtagt agcaattatt aaaaaactta
    17761 ctgctatttt attcataagt atccctctcc taattctctt tcgatactta ttataccata
    17821 gtatagatta tatttttata cttttaaatc aatttataaa ttattatagt aaattattta
    17881 caattctaag ctatttgaat ttatttttat agtatattct ttctctaaat tcgacagatt
    17941 tttattcaaa agccctttta tttccctcgt atttctatac ttgttgctat gttcttcatt
    18001 atgttcataa tcatatgaat cattgtcata ttcttcatca ttattaaatt cttcaattgc
    18061 ttcttttgac tttatatcac aattaaattt atacttattt actggtatat caagtcttac
    18121 cttagaattt ggaacttcta tgtccaggaa cttagcaatt ggcatatcag agtacaagta
    18181 aaattcactt tcataatttg aatttctacc tattttctct tttatattta cattttcagg
    18241 tatatactct tctatattat tgataaatac atctccattc tctgaagata tatccaatga
    18301 ctctgattct atgtttacat ttttaacgcc taaaattgac tttgttgata gatgaagatt
    18361 gctattagat attaagttta agttttctaa tctatttact ttattttctg ttatagctct
    18421 gctaaaacct ccagaagaag ttttatatgt tatattattt gaatctacat catcagatat
    18481 aaataagtac gaacttatac ttgatgcatt tatatctaca ttatttggta catatattct
    18541 aatttctcta taatcacgtg aaaacatact atttatagat gtatttaata agtggtcgaa
    18601 acttctaatc tttttaattt tattttcaga ttttctttcg ccttttacaa ctaaagtctt
    18661 atcttgtagg gttacactgt atttgtaaaa ttcattattc cctcttgttg tcacacgcac
    18721 atcttcattt gaactttttc ttatcataac atttatattg tcaacagcta tatctaactt
    18781 gtcaatattt actttttctt gataaagttt gttttctttg tttaattcag cttccttttc
    18841 taaaccaaag tttattaggc ttggcataac taaaatccct gaccatgttg tccctattat
    18901 acctattacc aataaaactg cagcaaatat agcaagtttt ttattcatgt ttatcttctc
    18961 ctttcttatt ttctatatct tcaaggtcct tatatatcgc ctcaaaattt atactatcat
    19021 cattatattc atcagtttct tttatgcttt cttcttctct attgtagtaa tcattattat
    19081 atgaattttc attaatgtct ttattcaaat cttcatcatt taaagtagct ttttttcttt
    19141 tttccatctt ttcatttgcc tttatataaa tattttttct ctttatccaa tgtatataat
    19201 tatttagtaa ttgtttacct agttttatgg caaataataa aatttgccat gcaagtatct
    19261 caagccctac aaatgctatg gttgcgaaaa tacaaaacat cataacatct ggaaccattg
    19321 ctataaattt aatcacaaat ggaattaata taacaaaagc aattaatgag catactaatg
    19381 caatagtagc agatacaata gcacatacaa acgcaaatgc tggaacaatt agaaataaag
    19441 ataacactac taataacccc tttataactt gtctggataa tcctcctaca ccttcttcta
    19501 tacttggtgt aagtttatca tttatatttt cttttgattt attgtacttt tccttagctt
    19561 catctttcaa gatagaataa tcctttttta catttgattt aaatctacta aatttaccat
    19621 ttaatatgtc tgtattcatt atttttccat ctatattttc ttttgattta ctatactttt
    19681 cttttgcttc atcttttaag atagagtaac cttttttcat atctgattta aatttactaa
    19741 acttatcatt caaaacatct gtatttatta ttttttcctc ttcaatattt ttttctttag
    19801 tttgtaagat aagttctttt gctatagatt ttggtgagcc aagtgatgct attatctcca
    19861 tatcactctt tccctctata agaccatcta cgaaatactc ttcataatct ctcaatatat
    19921 cattaacttc gctctcagaa aaatcttttt ttagataatc ctttaaaatt tcaagaaact
    19981 cagttttatt caagttcatt acctccctca tcttcactta ttaaaatctc aacagccttt
    20041 acaaattctc tccactcttc catcagattg gataaattct cttttcctag tgaagttatt
    20101 ctatagtatt ttctagcagg accttcatta gattctagta tatatgtttc gaaatacaaa
    20161 tcttttgtaa gtcttcttag aataggatat attgttcctt catttacatc tattacttta
    20221 gagatgttat gtactatctc atatccatac atatctttct tagatatgag agcaagtaca
    20281 caaatttcta aaacaccctt tctaaattgt gtgttcatat actccatcct ttctttaaat
    20341 acacagtatt gtgttttaca aggtactatg ttaaaacttc ttatatcctc ataataacac
    20401 agtactatgt tctgcacaat agttattttt aaatattctt gttgattaaa tccttctata
    20461 aatgtataat actctaatca gatatatttt atgaaagtgg tgataaattg gctaaaatga
    20521 aagatgataa aacaatatta atcctaactg ctcaatttgg tgctggacat ataagtgctg
    20581 caaaagcagt caaagaatgt attattgaaa aatatagtaa ctataatgtt gttatacaaa
    20641 actttataaa tgcaagtatt cccatgatga ataagcctat ggtaaaactt tatgaaaaca
    20701 atacaaaata tactcctgga ttatataatt actattatta ctttaagaaa tcgtttgatt
    20761 caagacatga tttatctcat aaattatata cacctaaact ttctgagtat attgctgata
    20821 taaatccaga tttgattatc tctacatttc cactagctgc tgcttgtgta aataacttta
    20881 agataaaaaa tcctgatata aatataccta cacttacagt tataacagat gttgtagata
    20941 gtatggaatg ggtttttgaa aatacagatt tgtattttgt tccttctcct gaaataaaaa
    21001 atagattttt tcagaaagga ataaatccag actccataaa ggttactgga gttcctgttg
    21061 ataagagatt tcaaattgaa agtaaagaac tatgttgtga taaatataga ctattacttc
    21121 taggtggagg aagaggtcta tttgatatag atgaggattt tatgcattgg atagatgaat
    21181 ttattgaaga acatagtaat tctatagaaa ttacaatagt tactggtaaa aacaaaaaat
    21241 tatatgacaa tctaactcat aaaaaacctc taaaaaatat taaagtactt ggatttgtaa
    21301 acgacatgta taatctaata agagaatgtg acctaatgct aactaagcct ggaggagcaa
    21361 ctatatttga agctattcaa tcacaaactc cagtactcgt aaaaatgcca aaagttggac
    21421 aagaaattga aaacgctaaa tttataatag ataagggtct tggcatgatt tatagtgatg
    21481 acctagacct taaaaacata ttttataaat tagtatcaaa tgaatttgat tctataataa
    21541 actttatgaa aaaaaattta aaagaattta aaacagtcat tcatccagag aaaattgctg
    21601 attatatatc agaattaata gataatcatt acagttaaat tgtactatta aaaaatttca
    21661 aacataaaat taaaggcaat caagtatgct tttacacttg agaatttact catagtgttt
    21721 aataatactt gattgccttt atcatatcaa aatcaaattg ttatattagt atatattttt
    21781 cttgtactgc atatacagtt gttgtatttc ttccatactt ctctttaatt ctatttgtaa
    21841 atctgaagca gtctggtaat ccttattttc tatagcttct ctcactctag tgaatagtga
    21901 ttctctagtt aaagtatcct tcataatatt gaactttaaa tccgatattg agttccaaac
    21961 tacctcatca agcgcatcca tattttcttt tgtgtgtatc ttgacaatac ttcttatttt
    22021 ttgaatccca gcctctatta ttctagtttt taatttcttt tgccacttat ctatagctcc
    22081 aatcttaaat gattctatta tactatcagt aaatacatca ccttgtttta aagattttaa
    22141 ttttgaagca tatatttcta agtttttcat attttcataa actgtagccg gtggtattcc
    22201 aaatcttgca tttctttctt ccaaactata atgttcaaat acatcatttt catctctata
    22261 tgctctatct ttttctaaat agaaactttc ttcacctaca tttttagata actctttttc
    22321 aagttctttt gtactaagac cactcttagc tgctgctttt ataccatcca acattgtttg
    22381 ataacatcct gctattacta aatacgtgtt agatagaggg cttggtgacc ttaactcaaa
    22441 tcttacagtc tttggatttt tcatatctct tattaatcca acaagcactg acctatttct
    22501 tgatggaatc tcataactat gacctaaaga tgtaactatg catacaggag cttcaaatcc
    22561 aggaactaat ctattaaatc catcattaga agctgttaca attgggttta atacttcata
    22621 attgtaaaga agtcccataa gagccccata tcctagttca cttagataat cttcttttaa
    22681 atcttttggc gcaaatagat tttttatact tccatccttt aacttcgcac ttactcctac
    22741 atgtgtatgt ccaccacttc ctgctactcc atgaattggt tttgctttaa atgtaacttc
    22801 tagtccatgt gatgtaaata catcctctat aacatctctt actaataatt cattgtctgc
    22861 tgcttgtaac ggtgtagaaa acttccaaga aacctctaat tgctccatag catggtttgt
    22921 tcttccatct atactaatag aactttgtat tcctccgacc tctttgtgag ccatttcagg
    22981 gtttacacct agcttttgaa gtataattaa acttttctct aaacaagttc ttataatacc
    23041 atgagttctt ttccagtatt gctcttttag gctttgtgat acatataatt tctctaagtc
    23101 agctttatcc tcaggagtat taacccagaa ctcaagttct gtagctgctg ttaacattat
    23161 ttcctctata tcatctactg aatctattcc tatgttattt attacatgtg gatattctct
    23221 aaatatttca tacattgact ttttaaagta cttatctgct ttttgaagta ctcctctaga
    23281 acatactttt ttattatcat gtattaaaaa tgcaggtatc tttaatgttc caacaggtag
    23341 acctacttct tcgtctatat attccatatt gtaatctaca taccaatgac aacttttatc
    23401 tggcattaaa tctactttcg cattatttaa tgtagctata ttataaagct caacacttga
    23461 accatcagtc tgtatagctg attccaaaaa tttatctata tcctccaaaa ataattctac
    23521 aggaatcttt tcatctgttg catttcctcc taaatcaacc cccattaagg aaacaaatct
    23581 tatattttta ttgcttttta ggatttctct taattcttca ttagtgtgtt tatctttctc
    23641 tattacgtat aatagactat ccatttttcc actctcccat aatttttatg tatattttat
    23701 tttgttaaat acaagataaa tttcattttc cttacattta tgaatttttc ttgcaaatta
    23761 ttcgtgtttt attcgtgttt ttatttatat tattatatat caagtataaa aagttcgcaa
    23821 tattttttat tggaaaaaat attatacata caatagttta acatttttac aaatatgggg
    23881 tatactaaaa taaaagtatt tttatttgaa aatttaattt tatttataat ataatctata
    23941 gt
//
LOCUS       DAYAPA010000054        23704 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.054, whole genome shotgun sequence.
ACCESSION   DAYAPA010000054 DAYAPA010000000
VERSION     DAYAPA010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23704
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.054"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(467..1339)
                     /locus_tag="ACGI59_001999"
     CDS             complement(467..1339)
                     /locus_tag="ACGI59_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFG9739785.1"
                     /translation="MEETNEMKDSKHLISNIQSQYTRLSKGQKLIAQYILNNYDKVAF
                     MTACKLGETVGVSESTVVRFANALGYSGYPKLQAALQELIKNKLTTVQRVEMAHDYSD
                     DFAILNKVLKSDIDNIRSTLEEIDERAFKEASNKLLRARKIYILGMRSSFVVAQYLGF
                     YLDIILDNVHIIRMDMGDAFEQIVRINEEDVIVAISFPRYSKKSYQIVNYAKEKGAHV
                     ISLTDSLFAPVASLADNTLLVKSNMASFVDSLVPALSISNALAISVGMKEKEDIKQHF
                     DDLEQIWKRYSVYE"
     gene            complement(1733..2311)
                     /locus_tag="ACGI59_002000"
     CDS             complement(1733..2311)
                     /locus_tag="ACGI59_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFG9739786.1"
                     /translation="MAYAKYLTKITDINKFYLENIINEGDIVIDATMGNGYDTRYLAE
                     KVGEKGFVYAFDIQEEAIKSTRKKLEKEGYTDRVKLIFDGHEKMNQYIKEEVSCVLFN
                     LGYLPRAKHSIITKPDTTLKAIKLSLEMLKENGVISIAIYTGHDGGQEEKDCIYNLVN
                     NLNQDEFNVLESKFLNQVNNPPQLILIEKKKA"
     gene            complement(2369..3085)
                     /locus_tag="ACGI59_002001"
     CDS             complement(2369..3085)
                     /locus_tag="ACGI59_002001"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFG9739787.1"
                     /translation="MRINKYIASCGIASRRKAEEIILGGRIKVNGSVVKELSFNIDEE
                     KDIVEFDNKKVKPSENYIYIVLNKPEGYITTVKDQFNRPSVIDILKDVRERVYPIGRL
                     DYETSGLLILTNDGDLTYKLTHPKHEIDKMYVASVKGIISDDEIRQFETGLKIEDYTT
                     APAKIRVTKENKEKNYSVCEITIHEGRNRQVRKMCKAINHPVLNLRRISVGKIVLKDT
                     KVGEYRYLTEDEIKYLKSIK"
     gene            3276..4361
                     /locus_tag="ACGI59_002002"
     CDS             3276..4361
                     /locus_tag="ACGI59_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFG9739788.1"
                     /translation="MIIISKENIKYYLIVVFIGILFFKFINTPSDFISSIEGFLKFFS
                     PFLLAILLALLLNPLVMLFEIKFKAHRLLAIFLSYIFIGFILAFAIRLLIPSIANTLN
                     RLINEMPMYTDYIDSFIEKNMSNIYFLKTLIPHIQHSLDNLLKEASNFVGKIPKNFLI
                     YTLSISSMLFNMTMGFILSIYIIYDKEKIALGFKRFLYSSTARNKADNIIEFFRTTHD
                     IFYDYLLGRILDSLIIGIIAFLGFQFVIRIENALFLASIIFLGNIIPYFGPFIGAIPP
                     IAMTILYSPQKTIWVIAFLFILQQLDGNFIEPKVMGNQVGIGAIWVISAILVGQSLFG
                     FIGVFLSVPIAAVVKTYVDKYIDNRLQ"
     gene            complement(4516..5175)
                     /locus_tag="ACGI59_002003"
     CDS             complement(4516..5175)
                     /locus_tag="ACGI59_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFG9739789.1"
                     /translation="MRGRVILLISILSIFLVGCSFNKKDEINLVEQGKTYLEKHNYKK
                     AMESLSSALEEDSTNENARAMYMQAMRMSNMTEFEELKNYEGAIKELKLIEKIKNGSS
                     VIKEEATKKKEELTKLEKEQNEAEEQRKKKAKDTAGEDRYRVESDARKSSYSGSSKNN
                     KSSSKNNDKKSDSNKSDNNNSSSQNQEKPNNSNPTPAPTPQDTTGGSDSGGNSQNSNN
                     Q"
     gene            complement(5392..6684)
                     /gene="lysA"
                     /locus_tag="ACGI59_002004"
     CDS             complement(5392..6684)
                     /gene="lysA"
                     /locus_tag="ACGI59_002004"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFG9739790.1"
                     /translation="MKLHGTMTVNNDELYIGGVSCLELSKKYQTPLYVFDEALVRQNC
                     REYIEYFKAREGKNKVAYAGKAFLPLYMCNLINEEQMCLDVVSGGELYTAYKSNFPME
                     KVFFHGNNKTKEEINMGLDLGVGKFVVDNFYELDLLEALCEDKGKTQEIYFRITPGIE
                     AHTHDYIKTGQIDSKFGFALVNGDLFRAIEKLSEYKNIKLVGLHAHIGSQIFDVEPYI
                     DTVDIMMNLVKEIKEKFNIQLTEIDLGGGVGVYYSEGDKPKTIKEFCETIITKAETSC
                     KELGIEVPTLVIEPGRSIVANAGSTLYTVGSIKDIKDVRVYVSVDGGMTDNVRPSLYQ
                     ASYECSIVNKINHEAMNHVTIAGKCCESGDILIGDINIMDIKSGDILITTSTGAYGYS
                     MSSNYNKIPRAAVVSVFDGNDKIICKRQSYEDLLALEI"
     gene            complement(6702..7895)
                     /locus_tag="ACGI59_002005"
     CDS             complement(6702..7895)
                     /locus_tag="ACGI59_002005"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HFG9739791.1"
                     /translation="MSIIVQKYGGSSVADTEKIKSIAENIIERRKENPQMVIVVSAMG
                     KSTDEYITLAKELSNEPSKRELDALMSTGEMISASLLSIALNALGCKAISYNAYQLNI
                     KTSGLHGKSQIDDINVNRIKNSLDEGNVVIVTGFQGINEDGDVTTLGRGGSDTSAVAL
                     AVKLNGKCEIYTDVDGIYFTDPRKYSKASKLDEIEYEEMLELASLGAQVMHSRSIELA
                     QKYGVEIYVGRTCGTEKGTYIRGGKDMKLEDKVITGLATSDDDSSITIKDFKAENISS
                     LFEDIATIGISVDMISQTAPILDKISVSFTVPKEELGECKKIVSKYTDEEHLVIDNNI
                     TKFSLVGLGMKNTSGVAAKVFKIFNENGIMIKLITTSEIRITCAINSDDKQVAIEKIA
                     EVFNI"
     regulatory      complement(7977..8145)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            complement(8256..8873)
                     /locus_tag="ACGI59_002006"
     CDS             complement(8256..8873)
                     /locus_tag="ACGI59_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9739792.1"
                     /translation="MTNMEKEILLLISRVRCVTESQFRKIYGDQKRYNKKNFKKTLRK
                     MCSEYTLKKYPCDVDYYNYKDLSYVYYLNGSKLFKGDDLIKALVGSELVVRINTAGCD
                     VKRFYRNVSVDDVKYDLFIEYTDQFGNIKQVLVDVDLNNEIDISKYENLSKKIDKSTI
                     PFFKVPNVLVVTPDNLSELNDLERYDNEYSINFIDLGLNKLFNCI"
     gene            complement(9043..10743)
                     /locus_tag="ACGI59_002007"
     CDS             complement(9043..10743)
                     /locus_tag="ACGI59_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9739793.1"
                     /translation="MKRYLKTYGVALIIVIIYAFIKLPVLRLDFTSLFSVGIIFFVVA
                     GILDMMLDRNERASKMAKYNFSIAIALLVYIVVVPFITSTPVLHAKSYRELLGKVTES
                     KFTDDISPVSVNDIRLVDEDMAMKLGDKKLGEVPAIGSVSKLGKFHIQNVDGELYWVA
                     PLVHRDIIKWITSLSGTSGYVKVSASNPQDVQLVQEIDGKPIKIVYQPEAYLHQDLQR
                     HLYIHGIVNVGMTDFTLEINDEGRPYWVVSLYEHKIGYGGANATGIATVDAETGKINV
                     YDVKNTPKWVDRIQPQSFVTDQIKDWGVYVNGFLNSVISEKGVLVPTEGTSLVYGNDN
                     RSYWYTGITSSGGDESTIGFMLVDSRTKEARLYKQPGATETAAMKSAEGNVQEKNYEA
                     TFPVMYNILGQPTYVSSLKDKAGLVKRVAFVSVEDYNVLGIGEDKNEALRNYKDALES
                     KGNDLKLDNDLKDEVLEGTVTRISPDVRGGNTNYYVTLDSNKDIIFRATSKVSSELPL
                     TQVGDKVKISYSKEESGVIEMSEFDNLNIANFEDKKAKEKTGTDENTDSKDSENQGNV
                     "
     gene            complement(11140..11703)
                     /locus_tag="ACGI59_002008"
     CDS             complement(11140..11703)
                     /locus_tag="ACGI59_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9739794.1"
                     /translation="MNKVYIVTTYTGTILSYLIRNISKKLYTHVSISLNENLKPMYSF
                     GRLNPRNPFIGGFVEENINQGLYAIRKNTVCRVYSLEVDNLQYENLYKNIKLISDYRE
                     DYYYDTMALIYLLMNIQRQSEYKYVCSNFVADMLEKSEIDILNKQAFEVTPNDFYNLS
                     GLTLEYEGLLSEYNSRQHSYVSKIANI"
     gene            complement(11862..12575)
                     /locus_tag="ACGI59_002009"
     CDS             complement(11862..12575)
                     /locus_tag="ACGI59_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2140 domain-containing protein"
                     /protein_id="HFG9739795.1"
                     /translation="MGKVAKILLSLIVIVLVLAGIVVYILTPKERYDVSSQNNKIIDE
                     EYLSKGSYINSMEVVKNPLRMVGKISVSEEEFKNLIYTLMQKHGINELENNFVEMKNG
                     KIKVAGPYKVFGLVNSQYELELRPTLKDGNLVVSLENVKIGKFKLSDKMLEKILSNYN
                     KKVPFEVNGNKITLEKSYLYPITLKNISIKKGNVDLDMEVEIDLKSQINGALGYLKDS
                     GKVQDILNHLINLKKTSVG"
     gene            12867..14531
                     /locus_tag="ACGI59_002010"
     CDS             12867..14531
                     /locus_tag="ACGI59_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HFG9739796.1"
                     /translation="MSNETNSSNFIKNIIINDLETGKHDSIITRFPPEPNGYLHIGHA
                     KSICLNFGLAKEFNGKANLRFDDTNPLKEDVEYVESIKEDVKWLGFDWNELNFASNYF
                     DEMYKRALILIKKGKAYVCDLTQEEMREYRGTLTEPGKESPHRNRTIEENLDLFERMK
                     NGEFKDGEKTLRAKIDMSSPNINLRDPIIYRISHSTHHNTGDKWCIYPMYAFAHPIED
                     AIEGITHSICTLEFEDQRPLYDWFVKECEMENIPRQIEFARLNINNTVMSKRKLKQLV
                     DEGIVDGWDDPRVPTISGIRRKGYTAEALRNFCSEIGVSKVNSTVDSQMLDYFLRENL
                     QPKAPLTMGVLRPLKLIITNYPEDKIEMLEIENNAKDESQGKRLVPFSRELYIEQDDF
                     MEEPVKKYFRFFPGNEVRLKGAYFVKCTDVIKDENGNVVEIHGTYDPETKSGSGFTGR
                     KVKSTIHWVDAKSAIPCEFRLFEPLILDDIPENEGKHFLKQINPNSLEILQGFVEPTQ
                     IKDAKPFDKFQFVRNGFFSIDNKYTTDEKFVFNRIVPLKSSFKPGK"
     gene            complement(14638..15537)
                     /locus_tag="ACGI59_002011"
     CDS             complement(14638..15537)
                     /locus_tag="ACGI59_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HFG9739797.1"
                     /translation="MKKVKLIYNPNSGEKKILSNLDTIIELYQEKNYLLIPYRLTIKE
                     PVKNAFKEVDSSYEHILIAGGDGTVDLVVNAMKELDIKIPIGILPTGTANDFSNALQI
                     SFDIKEAINEIINSKPKKIDIGKVNNKYFINVASAGMFTDVSQRINTDLKNSFGRISY
                     YIKGIEEALYMRKFKIKVSSEEMYYDGDMYLMLIFNGKTAGNINLAYKAEIDDGYLDV
                     IIFKGMPIPKSIPVLISVLRGDYLDQYNGNEILYFKTKKVHIECEDSLITDIDGEKGP
                     DFPLNIECIKDGIEVMGICSEKS"
     gene            complement(15564..16202)
                     /locus_tag="ACGI59_002012"
     CDS             complement(15564..16202)
                     /locus_tag="ACGI59_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HFG9739798.1"
                     /translation="MDQNLNWLNEKRIEKTIKSLENNNMKGYLVSNKEELLKKIEEIV
                     KAGSLVGCGGSMTLFETGVIDFIREKYNFLDRYAKDIKPEEMKEIYRKSFSADAYFTS
                     TNAITEDGELYNLDGNGNRVAAMIYGPDKVIVIAGVNKIVKDVDEAIKRTKTIAAPAN
                     CKRLSTNTGCKTTGICIDCSSPGRICCSYTLIKRQLTPERIHVIFLNEDFGY"
     gene            complement(16539..17537)
                     /locus_tag="ACGI59_002013"
     CDS             complement(16539..17537)
                     /locus_tag="ACGI59_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4003 domain-containing protein"
                     /protein_id="HFG9739799.1"
                     /translation="MEQSFINGKVNLLIENYKALNEVKGSWQMGLIQHSCALAFTLKN
                     KRISPRLVEERIELIKKNTGLFSNFRGYNMFYMATLLSFESNPESSFKMILDIYKELK
                     SEKFWGDTYLPLTASIVYENREKMDYLTCISKMKIIYDYMRKKHPFLTSSDDYCNIAL
                     IAIHSKNLDEDLEYIEKCYEFLNENGFYKGNDLQALSQILLFSDDRTMLKCKKTIELK
                     KAFKENDCKMNYYGYPIIGAISLLDYREDEIIEEIKNVSNKLKEEKGFGNWSLGKSNR
                     VMISSAIVASIYADLIRNENNIGSITNNIFLNIVVAIQIACVMAATSAAIASSSSN"
     gene            complement(17635..18210)
                     /locus_tag="ACGI59_002014"
     CDS             complement(17635..18210)
                     /locus_tag="ACGI59_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9739800.1"
                     /translation="MKLKEALITCVVCSVCLTPLLVNGETKIDADHCKESIVNIDTIV
                     KSGKSFNSKYNQGMEDYKNSDEYKLKVQKEKLQNQLGIKIKKLVPITFEVSYYTSLNQ
                     ENGYGAITATGDDLEDGFVANNHLSFGTKIIVDGKLKTVMDRGSSKYFSNSNAIDVFV
                     PKWSNESESEYYDRVNNMGRHYKKGYIIVEE"
     gene            18526..18789
                     /locus_tag="ACGI59_002015"
     CDS             18526..18789
                     /locus_tag="ACGI59_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9739801.1"
                     /translation="MIKNTKQITVEVNEILLQEMSKITNDENNILTDLINESIVNYIL
                     ENKKHNIENKDLEEVIKFYKKENTEKPKSAARAIAEAKTHTLY"
     gene            complement(19226..19603)
                     /locus_tag="ACGI59_002016"
     CDS             complement(19226..19603)
                     /locus_tag="ACGI59_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HFG9739802.1"
                     /translation="MTITKIPPAELKVMKFIWSSDFTVASKDVIEAMEKLYNWKQTTT
                     LTLLSRLVNKQFLSARKIDRHTHYTVLIGEEEYLHFETKTFLDNMHGSSIESFMKSLF
                     KKGVNEEELNSVEKWVKDIKKDE"
     gene            19944..21056
                     /locus_tag="ACGI59_002017"
     CDS             19944..21056
                     /locus_tag="ACGI59_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFG9739803.1"
                     /translation="MNAFIRKRNKNYVVYLEFEDEKTGKRKQKNMGTFDKKREASKRL
                     NEVKESLYNDGFLVPSEITVSEFLLDFLNKYSENISLATYNNYVSICKNYINPSIGKY
                     KIQDLHPVHIQNYIDKLSYKLNPQSIKIHINILKLAIKRAYRLKLIKENVMDSIEAPR
                     YKKFKNEIYDREQMIKLLDLAKNTEMELPINLAIGLGLRISEVLGLTWDNIDFEENTI
                     TVNKITSRINGSVILKEPKTESSVRKISSPKELMSLLKEYKIKQNKNLLKSSIRNNNN
                     LLFFNKKCEPIAEDVMSKKFKRFLEKNELPHIRFHDLRHSHVTLLINSKVPIKVISER
                     VGHSNISTTLSVYSHVLKEMDKEASDKISESLFNAN"
     gene            complement(21264..21740)
                     /locus_tag="ACGI59_002018"
     CDS             complement(21264..21740)
                     /locus_tag="ACGI59_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9739804.1"
                     /translation="MVKKRLVAVVISLAMISVGNFSYAHSGGHNSSGGHHNKNKSELT
                     RYYCGGHSAHQHNKGFCPYASTKENSGVTISSVHNEKNHKNYFAEKGYNKGYEDGCKG
                     LYNLSVTEAKSDNLYEKEILKSSYEKGYLIGYNEYKAKKVDDVMKKMLDIPMLASI"
     gene            complement(21939..23564)
                     /locus_tag="ACGI59_002019"
     CDS             complement(21939..23564)
                     /locus_tag="ACGI59_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFG9739805.1"
                     /translation="MLQVTGVGLRFGDKELFKDVNLKFTKGNCYGIIGANGAGKSTFL
                     KILSGEIEPNTGSISITDKERMSVLKQDHFEYEEETVLNVVIRGHERLWNIMKEKDAL
                     YMKEDFNEEDGIKAAELEGEFAELDGWDAETNAEKILMGLGITKDMHYKQMKELVGGE
                     KVKVLLAQSLFGKPEILLMDEPTNHLDFKSINWLNNFIMDLEESIVIIVSHDRHFLNQ
                     ICTNIVDVDFGKIQMYVGNYDFWYESSQLALQLAKDQNKKTEEKIAQLKEFIARFSSN
                     ASKAKQATSRKKQLEKLEVEDIQPSRRRYPYVGFTPAREIGNEVLEVHNLTKTIDGVK
                     VLDNVSFRLDRDDKVVFMGDEIATTALLNIVMGELEPDSGEYKWGVTTSQDYLPKNHN
                     KFFDGVEYSLVDWLRQFSEEKSESFIRGFLGRMLFSGEEALKEAQVISGGEKVRCMLS
                     KLMLSNANVLVLDDPTNHLDLESITSVNKGLEKFPGVLLFTSHDHEFISTIANRIIEI
                     TPNGIMDRKMDFDEYLESKEIQDQLAKMYGKDK"
BASE COUNT         7902 a         3854 c         2488 g         9460 t
ORIGIN      
        1 ttctaaaaaa gtttcccttt ccccaaaaat ggctacttaa aaaatcaaga tttcatacct
       61 aactactaat tctatgattt ttaaattttt caacttcttt gtcaaagtta aaataaaata
      121 ctaaaagaca catattatag tgaacaaaaa agatagaaat acatatttta atataatttt
      181 aataagatat tatatactta aatacaaata aaattaattt tataattgtt aattaaggaa
      241 atgatattgt ttagagaaat aaaaaaagca ctctccatga atggaaatgc tttctttaat
      301 aaattagttg acgtagtaaa gatgtattca gaatacatct ttatgttttg agtctcgaaa
      361 tatttatttg tattatttaa acgaaaattt attttttaag tgataatcga gtatttacta
      421 ataaggctat gattctcaat ttcatagcct tattaatata caaactttac tcatacacag
      481 aatatctttt ccaaatttgc tctaaatcat caaaatgctg tttaatatct tctttttcct
      541 tcatcccaac agatatagca agggcatttg aaatactaag agctggaact agagaatcta
      601 caaatgaagc catattactc ttaactagaa gagtgttatc tgcaagtgat gctactggcg
      661 caaataagct atctgttaat gatattacat gggctccttt ttctttagca taattgacaa
      721 tttgataaga tttcttagaa taacgaggga aactaatagc aactattaca tcttcttcat
      781 ttattcttac aatctgctca aaagcatctc ccatatccat tctaattata tgaacattgt
      841 caagtattat atctaagtaa aaacctaaat attgagctac tacaaaagaa ctcctcattc
      901 caagtatata tatttttctc gctcttaaaa gtttgttaga agcttcttta aacgctctct
      961 cgtcaatttc ttctagtgta gaccttatat tatctatatc actttttaaa actttgttta
     1021 gtattgcaaa atcatcggaa taatcatgtg ccatttcaac tctttgtact gtagttagtt
     1081 tattttttat gagttcttga agtgcagctt gtaatttagg atatccacta taacctaatg
     1141 catttgcaaa tcttacaact gtagactcac ttactcctac ggtttcaccc aatttacatg
     1201 cagtcataaa tgcaacttta tcataattat ttaaaatgta ttgtgctata agtttttgac
     1261 ccttactcaa tctcgtatat tgagattgta tatttgatat caaatgttta ctgtccttca
     1321 tttcatttgt ttcttccatt tttttctcct tttagttata taaactattt tgttttatta
     1381 acactgtctt tttctctaaa aattttattt tttatctcaa tttcatctta gtttttatca
     1441 tagcttctta aactcataaa attctaactt ttctgtacct tatacttcac atagtatttt
     1501 cgattaaatt tattatacca tctttccttg cattttttaa tatttatcca atatttcttt
     1561 taaatttttc aatttttact catttaaaag ttatctatct tattttgcat atttagatgt
     1621 atattttgtt ttgagaataa actgttgtat acttattatt taaagtattt ttaataggtc
     1681 acactatata tattaactct taatacaatc ttgtcaagtt ttttagtatt tattatgctt
     1741 ttttcttttc aattaaaatc aattgtggtg ggttattgac ttgatttaaa aacttagatt
     1801 ctaaaacatt aaattcatct tgatttaaat tattgacaag gttatatatg caatcttttt
     1861 cttcttgacc tccatcatgc cctgtatata tggcaatgct tattactcca ttttctttta
     1921 acatttctaa acttaacttt atagccttta aagttgtatc tggtttggtt ataattgaat
     1981 gtttcgctct tggaagataa cccaaattaa ataaaacaca tgaaacttct tcttttatat
     2041 attgattcat tttttcatgc ccatcaaata tcaattttac tctatctgta tatccttctt
     2101 tctctagttt ctttcttgta gactttattg cttcttcttg tatatcaaaa gcatatacaa
     2161 agcccttttc tccaaccttc tcagctagat accttgtatc atacccattt cccatagtag
     2221 catcaattac tatgtcacct tcatttataa tattttctaa ataaaattta tttatatctg
     2281 taattttagt taaatacttt gcatatgcca taatactatt tctccctact attaacttta
     2341 tttcattttt tctcaattta tttttatctt attttattga ttttaaatat tttatttcat
     2401 cttctgttag gtatctatat tctccaactt tggtgtcttt taatactatt ttccccacag
     2461 aaatccttct taaatttaaa actggatgat ttatagcttt gcacattttt cttacttgtc
     2521 tatttctacc ctcatgtata gttatttcac acactgaata atttttttct ttattttctt
     2581 tagttaccct tattttggca ggtgccgttg tgtaatcttc tatttttagt cctgtttcga
     2641 attgtcttat ctcatcatca cttataattc ccttcacact tgccacatac attttatcaa
     2701 tttcatgctt tggatgggta agcttatatg ttaaatcgcc atcattagta agtattaata
     2761 aaccactagt ttcataatct aatctcccta ttggatatac tctttccctt acatctttaa
     2821 gtatgtctat tacactgggt ctattgaatt ggtcttttac tgtagttata tatccctctg
     2881 gtttatttaa tactatatat atataatttt cacttggttt tactttttta ttatcaaatt
     2941 ctactatgtc tttttcttca tcaatattaa aagataactc ttttactaca cttccattta
     3001 cctttattct tcctccaaga attatttctt ctgcttttct tcttgatgct attccacaag
     3061 aagctatata cttattaatt ctcatactta atccttcctt atcttaaatt atgtgtgttt
     3121 aatattttta tcaatgttaa ttttgctaca ttattatact atactagacc ttaattaaaa
     3181 acaagcaaaa aaattattta attttcctaa ataattgtaa tttcttttca catactatta
     3241 atatgtaata gctgactaaa cactatttta aggaagtgat tattatttca aaggaaaata
     3301 taaaatatta tttaatagtc gtgtttattg gaattttatt ttttaaattt attaatactc
     3361 catcagactt tatatctagt attgaaggat tcttaaagtt ttttagccca tttttattag
     3421 ctattttact tgctttactt ttaaatccac ttgtgatgct ttttgagata aaattcaaag
     3481 cacatagact tttagccatt tttttatctt atatatttat aggttttatt ttagcctttg
     3541 ccattaggtt attaattcca tcaatagcta atacattaaa tagattgata aatgaaatgc
     3601 ctatgtatac tgattatata gatagtttta tagaaaaaaa tatgtcaaac atatattttc
     3661 taaaaacttt aattcctcat atacaacata gtttagataa tttattaaaa gaagcaagta
     3721 attttgttgg taaaattcct aaaaacttct taatctatac acttagtata tcttctatgc
     3781 tatttaatat gacaatggga tttatactat ctatatacat tatatatgac aaagaaaaaa
     3841 ttgcattggg atttaagaga tttctatatt cttctactgc tagaaacaag gcagataaca
     3901 ttatagaatt ttttaggact actcatgata ttttctatga ttatttactt ggtagaatat
     3961 tagactctct tattattggt ataattgcat ttttaggatt tcaatttgtt atcagaatag
     4021 aaaatgcttt atttttagct tcaataatat tcttaggtaa tataattccc tattttggtc
     4081 cttttattgg tgcaatccct ccaatagcca tgactatatt atatagtcca caaaaaacta
     4141 tatgggtcat tgcatttcta tttatattgc aacaattaga tggaaatttt atagaaccaa
     4201 aagttatggg taatcaagtt ggtataggag ctatatgggt aatatctgct attttggtgg
     4261 gacaatctct atttggtttc ataggagttt ttctttcagt accaatagct gctgttgtaa
     4321 aaacttatgt tgataaatat atagataatc gtttacaata gattatctct actgtgactt
     4381 taatttttag tttaaaattt aactgctatt tattaaatta tttctcaaaa aatatctctt
     4441 agtaagaaaa aattgctaag agatattttt tataatatat aatttgttca ttaaatttat
     4501 gcatatattt tatacttatt gattatttga gttttgagaa tttccaccac tatctgaacc
     4561 accagtagta tcttgtggag ttggtgctgg tgttggatta ctattatttg gcttttcttg
     4621 attttgagaa gaactgttat tattgtcaga cttattacta tctgattttt tatcattgtt
     4681 tttagaacta gatttattat ttttactact tccactataa gaagatttcc ttgcatctga
     4741 ttcaactcta tacctatctt ctccagccgt atcttttgcc tttttctttc tttgttcctc
     4801 tgcttcgttt tgttcttttt ctaatttagt taactcttct ttctttttag ttgcctcttc
     4861 tttaataaca gaggaaccat ttttgatttt ctctataagt ttaagttcct tgattgcacc
     4921 ctcataattt tttaattctt caaactccgt catatttgac attctcatag cttgcatata
     4981 catagctctt gcattctcat tagtactatc ttcttccagt gcagaggata atgattccat
     5041 agctttttta tagttatgtt tttctaaata tgtttttcct tgctctacta aatttatttc
     5101 atctttttta ttaaatgaac atcctacaag aaatatagat aatatcgata ttaataaaat
     5161 tactcttcct ctcattaaat ccctccttgt catatacttt aaattttatc ataattataa
     5221 agtttttaaa atattttata tttattacaa gaaatttgtt agtaaaacaa aaaataagca
     5281 gatatgctac gaacgataca ataaaccaaa aattaattta gagatacata tcctagcaaa
     5341 tctgctcata ataaacttta atatttaatt tttattatat acttctctat tttatatctc
     5401 taaagctaat aagtcctcat agctttgtct cttacaaatt atcttatcat ttccatcaaa
     5461 tactgatacc actgctgctc ttggtatttt attataattt gaagacattg aatatccata
     5521 tgcaccagtt gatgttgtta taagtatatc tccactttta atatccatta tgtttatatc
     5581 tccaattaat atatctccac tctcacaaca ttttccagct attgttacat gattcatagc
     5641 ctcatgatta attttattca caatactaca ctcataacta gcttggtaca acgaaggtct
     5701 tacattatct gtcatacctc catctacact tacataaacc ctaacatctt ttatatcttt
     5761 tatagaacct actgtataca atgtacttcc tgcatttgca actattgacc ttcctggttc
     5821 tattaccaat gtaggtactt ctattccaag ctctttacat gaagtttctg ctttagtgat
     5881 tatggtttca caaaactctt ttatagtttt aggtttatct ccttcgctat aataaacgcc
     5941 aactcctcca cctaggtcaa tttctgttag ttgtatgttg aatttttctt ttatttcttt
     6001 taccaagttc atcattatat ccactgtatc tatgtatggt tctacatcaa atatttgtga
     6061 acctatatga gcgtgaagac ctacaagttt aatattttta tattcactta atttttctat
     6121 tgctctaaat aaatctccat ttactaaagc aaatccaaat ttagaatcta tctgtccagt
     6181 ttttatataa tcatgtgtat gagcttctat accaggagtt attctaaagt atatctcttg
     6241 agttttacct ttatcttcac ataatgcttc aagcaaatct aattcataaa aattatccac
     6301 tacaaattta cctacaccta aatctaatcc catgtttatt tcttctttag tcttattgtt
     6361 tccatgaaag aaaacttttt ccataggaaa atttgattta taagctgtat ataactcgcc
     6421 tcctgaaact acatctaaac acatttgttc ttcatttata agattacaca tgtaaagagg
     6481 taaaaaagct ttccctgcat aagcaacctt attttttccc tctctagcct taaaatattc
     6541 tatatattct ctacaatttt gtctaactaa agcttcatca aatacataaa gtggagtttg
     6601 atatttttta ctaagttcta aacaactcac tcctccaata tataattcat cattatttac
     6661 agtcatagtt ccatgaagtt tcataatagc ctccccaaat tttatatatt aaatacttct
     6721 gctatttttt ctatagctac ttgcttatca tcagaattta tagcacaagt tatacgaatc
     6781 tctgatgtag ttataagttt tatcattata ccattttcat tgaatatctt aaatacttta
     6841 gcagcaactc cagaagtatt tttcatacca agacctacta aagaaaattt agtgatatta
     6901 ttatcaatca ctaaatgttc ttcatctgta tatttagata ctatcttttt acattctccc
     6961 aactcttctt ttggaactgt aaatgataca cttatcttat ctaaaatagg tgcagtttgg
     7021 cttatcatat ctacacttat acctatagtt gctatatctt caaataagct agatatattt
     7081 tcagctttaa aatcttttat tgttatagaa ctgtcatcat cactagtagc taatcctgtt
     7141 attactttat cctctaattt catgtctttt ccccctctta tatatgttcc tttttctgta
     7201 ccacatgtac gtccgacata gatttcaaca ccatattttt gtgcaagctc tatacttctt
     7261 gaatgcatta cttgtgctcc taaactcgct aactcaagca tttcttcata ttcaatctca
     7321 tcaagtttac tagcctttga atattttctt gggtctgtaa aataaattcc atcaacatct
     7381 gtatatatct cacattttcc atttaattta accgcaagag ctacagcaga tgtatctgaa
     7441 ccacctcttc caagagtggt tacatcacca tcttcgttga ttccctgaaa tccagttact
     7501 atcacgacat ttccttcatc taaactattt ttaattctgt ttacgtttat atcatcaatt
     7561 tgagactttc catgaagacc actcgttttt atatttaact ggtaagcatt ataacttata
     7621 gctttacatc caagtgcatt taatgctatt gataataaag atgctgaaat catttcacca
     7681 gtagacatta aagcatctaa ttccctctta ctaggttcat tactcaattc ctttgctaaa
     7741 gttatatatt catcagtgga ttttcccatg gcagatacaa ctattaccat ttgtggattt
     7801 tcttttcttc gttctattat attttctgct attgatttta ttttttcagt atctgctact
     7861 gaacttccac catatttttg aacaattata ctcatgcttt ctccccctca agctaaattt
     7921 tgataaataa aaacgcccaa aggtatactt tggacgtgta aatttcatag accaagtaac
     7981 cttgtagctc accactttaa aagtgacagc cttatgcata ttctgcataa gtccagaaaa
     8041 tttgtccgtc aacaaaattt cttcggctat ataccctttc atcaattatc aacgcttacc
     8101 agctcctatt gaatactatt gaatacagct acctctaccc taggctcatt atttttaatt
     8161 aatttattaa tatagtttta tcatattcta tataatattg caacaattat tttacaaaat
     8221 gtggcaaatc gatagttttc ttttctaaag ttagcttata tacaattaaa tagtttattt
     8281 aatcctaaat ctataaaatt tatgctatac tcattatcat atctctctaa atcatttaat
     8341 tcggatagat tatctggtgt aactaccaaa acgtttggta ctttaaaaaa tggaattgta
     8401 gatttatcta tttttttaga aagattttcg tatttagaaa tgtctatttc attatttaag
     8461 tcaacatcaa ctaaaacttg ctttatatta ccaaattggt ctgtatattc aataaataaa
     8521 tcgtatttca catcatctac actaacattt cggtaaaatc tttttacatc acaacctgca
     8581 gtattgattc taacaactaa ttctgatcct actagagcct ttattaaatc atctccctta
     8641 aaaagtttac taccgtttaa atagtataca taagagaggt ctttataatt ataataatcc
     8701 acatcacaag gatatttttt tagtgtatat tcactacaca tttttctcaa agttttttta
     8761 aaattttttt tattgtatct tttttggtct ccataaattt ttctaaattg agactctgta
     8821 acacatctaa ccctgctaat taaaagtaat atctcttttt ccatattcgt catagtacat
     8881 tctccttgaa gtattatttg caaagttgta ctaatattat attaatttta tgacattaaa
     8941 tttacaaaga attaaaataa aatagctaat ttttatagaa ttaaattcta taaaaattag
     9001 ctatcactat gttaaatact attatcaatc tttttattta aattatacat taccttgatt
     9061 ttcagaatct ttggaatctg tattttcatc agttcctgtt ttttcttttg cttttttatc
     9121 ttcaaagttt gcaatattta ggttatcaaa ttctgacatt tctattacac cagattcttc
     9181 ttttgaatac gatattttga ctttatcacc aacctgagtc aatggcaatt cgctagatac
     9241 ttttgaagta gccctaaata ttatatcttt atttgaatct aaagttacat aataattagt
     9301 gttaccacct cgcacatctg ggcttattct tgtcactgtg ccttctaata cctcatcttt
     9361 taaatcatta tcaagtttta aatcattacc cttagattct aatgcatcct tatagtttct
     9421 taatgcttca ttcttatctt cacctattcc aagtacatta taatcttcaa cagatacaaa
     9481 tgcaactctc ttaacaagac ccgccttatc ttttaatgat gatacatatg ttggctgacc
     9541 caatatatta tacattacag ggaacgtcgc ttcataattt ttttcttgaa cattaccttc
     9601 agctgatttc attgcagccg tttcagtagc tcctggttgt ttatataatc tagcttcttt
     9661 tgttctagaa tcaactaaca taaatccaat tgtagattca tctccacctg aagatgttat
     9721 acctgtatac caataagacc tgttatcatt gccataaact aatgatgtac cttctgttgg
     9781 tactaaaact cccttttctg atataacaga gtttaaaaat ccattaacat aaacacccca
     9841 gtctttaatt tggtctgtta caaagctttg tggttgtatt ctatccaccc atttaggcgt
     9901 gtttttaaca tcataaacat ttatttttcc agtttctgca tcaacagtgg ctattcctgt
     9961 tgcatttgct cctccatagc caattttatg ttcatataaa cttacaaccc aataaggtct
    10021 tccttcatca tttatttcaa gagtgaaatc tgtcatacca acatttacaa taccatgtat
    10081 gtataaatgt ctttgtaaat cctgatgtaa gtaggcttct ggttggtaaa ctatctttat
    10141 tggctttcca tctatttctt gaactaattg gacgtcttga ggattactag ctgatacttt
    10201 tacataacca cttgtaccac ttagagatgt tatccattta attatgtctc tgtgaacaag
    10261 aggcgctacc cagtacaact ctccatctac attttgtata tgaaattttc ctaatttgga
    10321 tacactccct attgcaggaa cttctccaag ctttttgtca cctaatttca ttgccatatc
    10381 ttcatctaca agacgaatgt catttacact gacagggcta atgtcatcag taaatttact
    10441 ttcagttact tttcccaata gttctctata tgattttgca tgaagtacag gtgtagaagt
    10501 tataaaggga actactacaa tatacacaag caaagctatt gctattgaaa aattatattt
    10561 agccatttta gatgctcttt catttctatc taacatcata tctagtattc ctgctacaac
    10621 aaagaaaatt attcctactg aaaataaaga tgtaaaatct aatcttagta caggtaattt
    10681 aataaatgca taaattatta caattattaa tgcaactcca tatgttttaa gatatcgttt
    10741 catttaatac tcctctctaa aaaatgttat tattactatt atatccatat atttacaaaa
    10801 aataaatatt tatcatattt attatatatt aagatatctt tatatattta tagttctgct
    10861 tattcaacta tatttaacat tatacgtgta tgtataaata ctttttatac actgtttgtg
    10921 ctatccatcc cgcacctaaa ggagtgggat ttttgcacta ttctgtatta tatatatcca
    10981 aaaatgttat aaaaatacaa atattagcag aaatttaaaa actgaagaaa gtattctaac
    11041 taagatttac aaattcttaa tcctatcgaa gttaaactac tatcttcagc ctaattatct
    11101 agcaatattt ttttatatga aaaactctaa atcaagtttt taaatgtttg cgatttttga
    11161 tacataagaa tgttgtctag aattatattc tgataataaa ccttcatact ctaaggttaa
    11221 gccagataaa ttataaaaat catttggcgt tacttcaaat gcttgtttat ttaaaatgtc
    11281 tatctcactt ttttctaaca tgtcagcaac aaaatttgaa catacatatt tatattcaga
    11341 ttgtctctga atattcatta gtaaatatat taatgccatt gtatcgtaat agtaatcttc
    11401 tctataatca ctaattaatt ttatattttt atatagattt tcatattgta aattatctac
    11461 ttctaacgaa tataccctac acacagtgtt ttttcttata gcatataatc cttgatttat
    11521 attttcttca acaaatcctc caataaatgg attccttgga tttagccttc caaaagaata
    11581 cattggtttt agattttcat ttaatgatat tgacacatgt gtatacagct ttttacttat
    11641 attccttatt aagtaagata atatagttcc tgtatatgtt gttacaatat agactttatt
    11701 catcatttcc ccccaatcta gtaactaaat aatattatac acttacaatt taatatcata
    11761 agatattcta acataataaa tgtggtttta caaattttga ttttaaggta ctttaatatt
    11821 atataatata ataaaaatat aaatataagt tttctatatt tttaaccaac agatgttttc
    11881 ttcaaattaa tcagatgatt tagtatatct tgaacctttc cagagtcctt taaatatcct
    11941 aatgccccat ttatttgtga ttttaaatct atttctactt ccatatccaa atcaacattt
    12001 ccttttttta tagatatatt tttaagtgtt attgggtaaa gataactttt ttccaaagta
    12061 atcttatttc cattaacctc aaatggaact tttttattgt agttacttaa tattttttct
    12121 agcattttat cacttagttt aaattttcca attttcacat tctcaagact tacaactaag
    12181 tttccatcct ttaaagtagg tctcaattca agttcatatt gactatttac caaaccaaat
    12241 accttatatg gtcctgctac ctttatttta ccatttttca tttcaacaaa attattttca
    12301 agttcattaa ttccatgctt ctgcatcaat gtatatatta aatttttaaa ctcttcctct
    12361 gaaacagata tttttccaac cattctaagt ggatttttta ctacctccat agaatttata
    12421 tatgagcctt ttgataaata ttcttcatct atgattttat tattttgtga actaacatca
    12481 tatctttctt ttggtgtcaa gatatataca acaattcctg caagtactaa aacaataaca
    12541 attaaactta acaaaatctt tgcaaccttt cccataatcg tctcctttac aaatctgtca
    12601 tcagatttaa aataaattta gataactaac ttcacataat attcttaaca atatacatac
    12661 tataatacag tcttttaaaa attaattcaa ccaatctaat gttttgtaaa taaactgtaa
    12721 aaacatgttt ttagtataaa agttaatatt ttgcataata ctcttaatta cacctttatt
    12781 tttttctaat cttggattaa aattaatatt atgcaataca gtgtagtgtt ctgtattata
    12841 atcaaattaa ataggaggta tagaatatgt caaatgaaac gaactcatct aactttataa
    12901 agaatattat tataaacgac ttagaaacag gaaaacatga cagtatcata actcgttttc
    12961 cacctgagcc aaacggatat ttacatattg gtcatgcaaa aagtatatgt cttaattttg
    13021 gattagctaa ggagtttaat ggaaaagcta atttaagatt tgatgataca aacccattaa
    13081 aagaggatgt tgaatatgta gagtccataa aagaagatgt aaagtggtta ggatttgatt
    13141 ggaacgagtt aaattttgca tctaactact ttgatgaaat gtataagaga gctttaatac
    13201 taataaaaaa gggtaaagct tatgtatgtg atttgacaca agaggaaatg agagaatatc
    13261 gtggtactct aactgaacca ggaaaagaaa gtcctcatag aaacagaact attgaagaaa
    13321 accttgattt atttgaaaga atgaaaaatg gtgaatttaa agatggtgaa aaaacattaa
    13381 gagccaaaat agatatgtct tctccaaata taaaccttag agacccaatt atatatagaa
    13441 tatctcattc aacacaccat aatacaggag ataaatggtg catatatcct atgtatgctt
    13501 ttgctcatcc aattgaagat gcaattgaag gaataactca ctctatatgt actttagagt
    13561 ttgaagacca aagacctctt tatgattggt ttgttaaaga atgtgagatg gaaaatatac
    13621 ctagacaaat agaatttgct agacttaata taaataatac tgtcatgagt aaaaggaaat
    13681 taaaacaact tgtagatgag ggtattgttg atggttggga tgaccctcgt gttcctacaa
    13741 tatccggaat cagacgtaag ggatatactg cagaagcatt gcgcaatttc tgtagtgaaa
    13801 taggggtatc aaaagttaac tctacggtag atagtcaaat gcttgattat ttcctaagag
    13861 aaaacttaca accaaaggct cctcttacta tgggagtttt aagaccttta aaacttatta
    13921 taactaatta cccagaagat aagattgaga tgcttgaaat agagaataat gctaaagatg
    13981 aatctcaagg aaagagatta gttcctttct ctagagaatt gtatatagaa caagatgatt
    14041 ttatggaaga acctgttaag aaatatttta gatttttccc aggaaatgaa gtccgtttaa
    14101 aaggtgctta ttttgtaaaa tgtactgatg taattaagga tgaaaatgga aatgtagttg
    14161 aaatacatgg tacttatgac cctgaaacta agagtggctc tggctttaca ggacgtaagg
    14221 taaaatctac aatacattgg gttgatgcta aatcagctat accatgtgaa tttagattat
    14281 ttgaaccatt aattcttgat gatatacctg aaaatgaagg taaacatttc ttaaaacaaa
    14341 taaatcctaa ttcattggaa atattacaag gttttgttga acctacacag ataaaagatg
    14401 ctaaaccttt tgataaattc caatttgtta gaaatggatt ctttagtata gataataaat
    14461 atacaactga tgaaaaattt gtatttaata gaatagttcc tctaaaaagt tcttttaaac
    14521 caggtaaata atcttacaat caaaaaatga agacttaaaa tataaattac ttctactata
    14581 aataaactta gttagaagta attttttatt tgagtcttca tcatttactt taaatgatta
    14641 acttttttca ctacagattc ccatgacttc tattccatct tttatgcatt ctatgttaag
    14701 aggaaaatct ggtccttttt ctccgtcaat atctgttata agtgaatctt cacattctat
    14761 atgcaccttt ttagttttaa agtatagtat ttcatttcca ttatattgat ctaaataatc
    14821 acctctaagc acacttatca aaactggtat tgattttggt attggcattc ctttaaaaat
    14881 aattacatcc aaatatccat catctatttc agccttatat gctagattta tatttcctgc
    14941 tgttttgcca ttaaaaatca acataagata catatctcca tcataataca tttcctcaga
    15001 agatactttt atcttaaatt tcctcatata caatgcttct tctattccct ttatataata
    15061 agaaattctt ccaaatgaat tttttaagtc tgtgtttatc ctttgtgata catccgtaaa
    15121 cataccagca cttgcaacat ttataaagta cttattattg actttcccta tgtctatttt
    15181 ctttggtttt gaatttatga tttcatttat agcttctttt atatcaaatg aaatttgaag
    15241 agcatttgaa aaatcatttg cagttcctgt aggcaaaatt ccaattggaa tttttatgtc
    15301 taattctttc attgcattta ctactaaatc tacagtacca tcaccaccag ctatcaatat
    15361 gtgttcataa gaactatcaa cttccttaaa tgcattcttt acaggttctt taatagttaa
    15421 tctatagggt atcaataaat aatttttttc ttgatataat tctatgatag tgtctaaatt
    15481 tgataatatc tttttttcac ctgaattagg attgtatata agttttactt ttttcattat
    15541 ttctccttac tcattttatt cttttagtat ccaaaatctt catttaaaaa aatcacatgt
    15601 attctttctg gtgttaattg tctttttata agtgtataac tacagcatat tcttcctgga
    15661 cttgaacagt ctatacatat accagtagtc ttacatccag tatttgtact taatctttta
    15721 caattggctg gagcagctat tgtttttgtt ctttttatag cttcatctac atctttaact
    15781 attttattta ctcctgctat aactattact ttatcaggtc catatatcat agcagcaact
    15841 ctattcccat tcccatctaa attgtaaagt tctccatcct ctgttatcgc attagtactt
    15901 gtaaaataag catctgcaga aaatgacttt ctatatattt ctttcatttc ttctggtttg
    15961 atatcttttg catatctatc aagaaaatta tatttttctc ttataaaatc aatcactcct
    16021 gtttcaaata aggtcataga acctccacat cctactagag aacctgcttt aactatttct
    16081 tcaatttttt taagaagctc ttctttgtta cttactaaat accctttcat attattgttt
    16141 tccaaagatt ttattgtttt ttctattctt ttttcattaa gccaatttaa attttgatcc
    16201 attttaactt cccccttatt tttaatatgc acttaattat ttataaactt tattttcttt
    16261 ataagtttat tttagtacat ctaaaaaaaa atacaatata aaacttaaat agttttacat
    16321 aattttttcc aaaataactt actcaatcaa aaaaggaaag tagatgtact attctacttt
    16381 ccctatattg tatgtagtat ttattgccta aaatctatat attaattata tgtttcatat
    16441 tatataatat cacagttaat taaaatgtaa tactatcttc tttttaaaca tataaattgt
    16501 attattaaat ttaatatatc caacttcaat aaatcttatt aatttgatga agaagatgct
    16561 atagctgcac ttgttgctgc cattacacat gcaatttgaa tagcaacaac aatatttaaa
    16621 aatatattat tagttataga gcctatatta ttctcatttc gtatcaaatc tgcataaatt
    16681 gaagctacaa tagcagaact aatcattact ctattacttt ttcctaaaga ccaatttcca
    16741 aagccttttt cttctttaag tttatttgaa acatttttta tctcttctat tatttcatct
    16801 tctctataat ctaataatga aattgctcct attattggat atccataata attcatttta
    16861 caatcatttt ctttaaatgc ttttttaagt tcaatagtct ttttacattt aagcatagtt
    16921 ctatcatcac taaataaaag aatttgggat aatgcttgta aatcatttcc tttataaaaa
    16981 ccattttcat ttagaaattc ataacatttt tctatgtatt ctaaatcttc atctaaattt
    17041 tttgaatgaa ttgcaataag tgctatatta caatagtcat cactagaagt tagaaatgga
    17101 tgttttttcc tcatgtaatc atatattatt ttcatttttg atatacatgt aagataatcc
    17161 attttctctc tattttcata aactattgat gctgtaagtg gtaaatatgt atcgccccaa
    17221 aacttttcac ttttaagttc tttgtatata tctaaaatca tcttaaaact actttctgga
    17281 tttgattcaa acgaaagtag tgttgccata taaaacatat tatatcctct aaaattagag
    17341 aataatccag tatttttttt aataagctct attcgttctt ccactaatct aggacttatt
    17401 ctcttgttct taagggtaaa tgcaagtgca caactatgtt gtatcaatcc catttgccaa
    17461 gaacctttaa cttcatttaa tgctttgtaa ttttctatta ataagtttac ttttccattt
    17521 ataaacgatt gttccatatt gccctcctag taatatatct cattgtacta atagttttat
    17581 atttattata tatatattaa atactacaat caatacatat tacttcaaag aacatcattc
    17641 ttcaacaata atgtagcctt ttttgtaatg tcttcccata ttattgactc tatcatagta
    17701 ctctgattca ctttcattac tccatttggg cacaaataca tctattgcat tagaattact
    17761 aaaatactta ctagaccccc tatccataac agtttttaat tttccatcta caataatctt
    17821 agttccaaaa cttaagtgat tgtttgctac aaaaccatct tctaaatcat ctcctgtagc
    17881 agttatcgct ccatatccat tttcttgatt aagacttgtg taataactaa cttcaaaagt
    17941 tataggtact aacttcttta ttttaatacc taactgattt tgtagtttct ccttttgtac
    18001 tttcaactta tactcatcac tatttttata atcttccata ccttgattat acttggaatt
    18061 aaagctttta cctgacttta ctatagtatc tatatttact atagattctt tacaatgatc
    18121 tgcatctatt tttgtttctc catttaccaa taatggagtt aaacatacac tacatacaac
    18181 acatgtaatc aatgcttctt taagcttcat aaattccccc cttattattt tgatatatta
    18241 gcatatttaa tattatatgt ctacatttag atttaaatac cacacccatt tgctttaaga
    18301 agttgtctta tatttaattc accttaaaca cttataaatc ctctatctta gaatcatata
    18361 gaataaagct tttatcttta aatatttttt attaatcttt taaaatctaa aactacttta
    18421 tctaattttc tcttttttag aatttaagaa ttaaaattct ataattttaa aacatattga
    18481 atttattttt ttgagatgat ataattttct taagaggtgg ttgatatgat aaaaaataca
    18541 aaacaaatta cagtagaagt taatgaaatt ttattacaag aaatgtctaa gataactaat
    18601 gacgaaaata atattttgac agatttgata aatgaatcaa ttgtaaatta tattcttgaa
    18661 aacaaaaaac ataatattga aaataaagat ttagaagaag tgattaaatt ttacaaaaaa
    18721 gaaaatactg aaaaacctaa aagtgcagca agagctatag ctgaagcaaa aactcatact
    18781 ctgtattaat caaataaaaa agttcctaaa aactaggaac ttttttatat taataattat
    18841 taatatatct caatatttat ctcaaatcta tcaatataag catgtttaat acaatctatt
    18901 gtatacttat cttaccctag aaataatcaa aacaaaaata aaattatgcc ctttatatct
    18961 aaaacaatta atttacatga ttaatataca aaaattctga cttttaaatt cgtatacaat
    19021 atcaaactaa aataatatta atgacaaacc caatataaat taaattcaaa caggtttgat
    19081 tttacatcaa acttattgta ttaatattaa ataacaagac aaaaatattt ataaaaatca
    19141 caaattttac ataaaacttt atatttgatt catttaacta attattgtaa aaactattta
    19201 ttttatgata attaactaca ccaaattatt catctttttt aatatcctta acccattttt
    19261 caacagagtt caattcttct tcatttaccc cctttttgaa caaggatttc ataaaactct
    19321 caatagaact tccatgcata ttatctaaaa aagtttttgt ttcaaaatgt aaatactctt
    19381 cttctccaat aagaactgta taatgtgtat gtctatcaat ttttcttgca cttaagaatt
    19441 gcttatttac taatcttgat aaaagtgtta gtgttgtagt ttgcttccaa ttatatagtt
    19501 tttccatagc ttcaatgaca tcttttgatg ctactgtaaa atctgaactc caaataaatt
    19561 tcatgacttt taattctgct ggtggtatct tagtaatagt catatgattt tacactcctc
    19621 tcaaacttct acataattaa atactatatt aatagacttt tcgagtcaaa ggttttattt
    19681 aaaatatctc atccaaattt attaatacaa aaaatttata tttttattca aaattaaaca
    19741 cacaatctat taatataaag ataaatatct attctataag tataacagat tatcaaatta
    19801 aaattctaat atttgacata atatatacat tttttaaata aatatataca atgtattaaa
    19861 atgtatgcaa ctctatttca aaattatttt gtgatatata tatgttataa taaatgtata
    19921 aaattttgat tggtgggtta tatatgaatg catttattag aaaaagaaat aaaaactatg
    19981 ttgtttacct ggaatttgaa gatgaaaaga caggtaaaag aaaacaaaaa aatatgggta
    20041 cttttgataa aaaaagggaa gcaagtaaaa gactaaatga ggtaaaagaa agcttatata
    20101 atgatggttt tttagtacca agcgaaatta cagtgtcaga atttctatta gattttctaa
    20161 ataaatatag tgaaaatata tctttagcta catataataa ttatgttagt atttgtaaga
    20221 attacattaa tccatctatt ggaaaatata aaatacaaga cttacatccg gttcacatac
    20281 aaaactacat agataaacta tcttataaac taaatcctca atcaattaag attcatataa
    20341 atatattgaa acttgcaata aaaagagctt atagactcaa attaataaaa gaaaatgtta
    20401 tggatagtat agaggctcca agatataaaa aatttaaaaa tgagatttat gatagagaac
    20461 aaatgataaa attattagat ttagcaaaaa atacagaaat ggaattacct attaatttgg
    20521 ctattggatt aggtcttaga atttcagaag ttttaggtct aacttgggat aatattgatt
    20581 ttgaagaaaa tacaataaca gtaaataaga taacttcccg aattaatggt tctgttattc
    20641 tcaaagagcc aaagacagaa agctctgtta gaaaaatatc ttctcccaaa gaattaatgt
    20701 ctttattaaa agaatataaa ataaaacaga ataaaaattt attaaaatct tccattagaa
    20761 ataataataa tttgttattt ttcaataaga aatgtgaacc tattgctgaa gatgttatga
    20821 gtaaaaaatt taaaagattt ttagaaaaga atgaattacc tcatattaga ttccatgatt
    20881 taagacactc acatgttact ttacttataa attctaaagt acctataaag gttatatccg
    20941 agagagtagg acactcaaat ataagtacta ctctaagcgt ttattctcat gtacttaaag
    21001 aaatggataa agaggcttct gacaaaattt ctgaaagttt atttaatgct aattaatatt
    21061 aatttatctt agctttaaat tagcttagct tattgagata gattcattaa tattaaatgt
    21121 atctatctca aattaataac tacttaaata atctgtttta ttttaattta tcgcctcagt
    21181 tattatactt ttttacgaaa aattatactt tacaataaaa aagatattcc cttatagaaa
    21241 atatcttttt aaatactata ttattatata cttgctaaca ttggaatatc taacatcttc
    21301 ttcattacat catctacttt ttttgcttta tattcattgt atccaattaa atatcctttt
    21361 tcatatgaac tttttaaaat ctctttttca tataaattat cagatttggc ttctgtgaca
    21421 cttaaattat aaagcccttt acacccatct tcataacctt tattataccc tttttctgca
    21481 aaatagtttt tatgattttt ttcgttatgt actgaagata ttgttactcc agaattttct
    21541 tttgtactag cataaggaca aaaaccttta ttgtgttgat gtgctgaatg tcctccacaa
    21601 taataacgtg ttaactctga tttattttta ttatgatgac ctcctgatga attatgccct
    21661 cctgagtgag catatgaaaa attccctaca ctaatcatag ctaaactaat tactacagct
    21721 actaaccttt tcttaaccat aatctattcc ccccaaaatg ttatatattt ttattatatc
    21781 atctactata ttatatttaa attcttaata tacaaaatat tactctattc tgctaataaa
    21841 catgtttaaa caatataaaa aacctcctaa tatttgatta aaaatttaca cactcatgca
    21901 tagcatgtct caaatattaa aaggttttat actaataatt atttatcttt tccatacatt
    21961 ttagctaatt ggtcttgtat ctctttactt tctaaatact catcaaagtc catttttcta
    22021 tccattattc catttggagt tatctctata attctatttg ctatagtaga tataaattca
    22081 tggtcatgag aagtaaataa aagcactcct gggaactttt caagtccttt atttacagaa
    22141 gttatacttt ctaggtctaa gtggttagta gggtcatcta atacaagtac atttgcattt
    22201 gaaagcatta gcttactaag catacatcta accttttctc ctccagatat aacttgagct
    22261 tcttttaatg cttcttcacc tgagaataac attctaccta aaaatcctct tataaaactt
    22321 tcactttttt cttcagagaa ctgtctaagc cagtcaacca aagaatattc tactccgtca
    22381 aaaaacttat tatgattttt tggaagataa tcttgagaag tagttactcc ccatttatac
    22441 tctccactgt ctggttcaag ttctcccata actatgttta aaagtgcagt agtagctatt
    22501 tcatctccca taaatactac tttatcatct ctatccaatc taaatgacac attatcaaga
    22561 accttaaccc catctatagt tttagttaaa ttgtgtactt ctaaaacttc attcccaatt
    22621 tctcttgcag gtgtaaatcc tacatatgga tatcttcttc ttgatggttg tatatcttca
    22681 acttctaatt tctctaactg tttctttctt gatgttgctt gtttagcctt tgaagcatta
    22741 gaactaaatc tagctataaa ttcctttaat tgagctatct tttcttcagt ctttttattt
    22801 tggtccttag ccaattgtaa agccaattga cttgactcat accagaaatc atagtttcca
    22861 acgtacattt gtattttacc aaaatctacg tcaactatat tagtacatat ttgatttagg
    22921 aaatgtctat catgtgatac tattataact atagattctt ctaaatccat tataaaatta
    22981 tttaaccagt ttattgattt aaaatctaag tggtttgtag gttcgtccat taatagtatt
    23041 tcaggcttac caaaaagtga ttgtgctaac aataccttaa ccttttcccc accaactagt
    23101 tccttcattt gtttgtaatg catatctttc gttatgccaa gacccataag tattttctca
    23161 gcattagttt ctgcatccca tccgtcaagc tctgcaaact caccttcaag ttcggcagct
    23221 tttatcccat cttcttcatt aaagtcttcc ttcatataca atgcatcttt ttctttcatt
    23281 atgttccaaa gtctctcatg acctcttata acaacattta aaacagtttc ttcttcatat
    23341 tcaaagtggt cctgttttaa aactgacatt ctttctttat cagttataga tatactacct
    23401 gtattaggct ctatttctcc tgataatatt tttaaaaaag tagattttcc cgcaccatta
    23461 gctcctatta ttccatagca attaccttta gtgaatttta agttaacatc tttaaatagt
    23521 tccttatcac caaatctaag tccaacacct gtaacttgta acattaatat attttccttt
    23581 ctttttgctc ataatttatt ttatcataaa actttgataa catttttatt atatattatc
    23641 acgtaaatag tatatttttt atactaaatt tgtgaaatat ttattaaaat aatactaaaa
    23701 attg
//
LOCUS       DAYAPA010000055        23619 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.055, whole genome shotgun sequence.
ACCESSION   DAYAPA010000055 DAYAPA010000000
VERSION     DAYAPA010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23619
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.055"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(68..1984)
                     /locus_tag="ACGI59_002020"
     CDS             complement(68..1984)
                     /locus_tag="ACGI59_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFG9739806.1"
                     /translation="MNIKQFAINNISRNIKAYLGYLASIVISSSLLFSFIMFTSHPDL
                     DVSQFPDYLKEGLKMSKIIAYLFLFFFVFYSVSVFLKSRYKEFGILYITGISKRQVMK
                     LIFIENMIINIVSSVIGIIIGLIFAKIFLVIMSTFLELSPLKFYIPFNAILSTLIYFM
                     ILAVLTSIFTSFIVKENQVLKLLKGTKTPKPEPKTSLMLAILSICLFIIAYYNAITST
                     DQYTTYLRLVPVTSLTIVATYLFFSQFSVFFMKKLKLNKRFYRKNTNMLWISNLIYKV
                     KDNARIFFLITITSAVAFTAIGTVYSFWKDVERQINLIYPNTIYYSTMTLHNDPKKPD
                     SKEKDKERMMFIENNLKKENVDYTRTNGEFKTVFPKKSDFNVRIMKESKYLEITETIG
                     VKSISFEDNECISLLSSSLPGNNNVKENIIVGNRGLKVAKQVEECVMPAYYKNMYVVK
                     DNFYDSIESKFIVDRFTAFEVEDSSKTIEICREFENKFNNESGMQPYLFFSKELMIGT
                     GKLMYSTFIFLAIFIGLIFFVTTSSFLYNKFYMDCQVDKKKYEQLNKLGMTYNEIKKA
                     STIEIGIVFLFPYVVAVIHSIFALTALKNSFDIEVASSAVLVMGSLFLVQIVYFFIVR
                     NNYLKEIRLNLVNF"
     gene            complement(1984..2730)
                     /locus_tag="ACGI59_002021"
     CDS             complement(1984..2730)
                     /locus_tag="ACGI59_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9739807.1"
                     /translation="MKILTVNNLSKVYGKKIIFNALNDINFSIEDGEFVGIMGPSGSG
                     KTTLLNMISTIDKPTTGTMELKGKNPLLLRGEELALLRRRELGFVFQDFNLLDTLTIG
                     ENIVLPLTLDKVSVKEQDERLNEVSTILGIKDLLGKRTFEVSGGQAQRTAIARALINN
                     PSILLADEPTGNLDSKSSKVVMELFQKINKENKVTTMMVTHDPLAASYCSRILFIKDG
                     SIYNEIYKGSSREQFYQEIMDVLTLLGGDN"
     gene            complement(2834..3685)
                     /locus_tag="ACGI59_002022"
     CDS             complement(2834..3685)
                     /locus_tag="ACGI59_002022"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFG9739808.1"
                     /translation="MSLDVIFILKSVIIAIVEGLTEFIPVSSTGHMILVGNLIDFKGQ
                     FAEMFEVVIQLGAILAVVVLYWKKIKDSVIEFFKFIFTGGKEGKIGFRFGMNVIIGCI
                     PFAIIGVLFYDNIKSLFNLQSVIIGFIVGGILLLVVETLFRKKNHSTDNIDKITPIQA
                     LKVGTLQVLSAWPGMSRSASTIMGGWIAGLNSPTAAEFSFFLAVPAMVASSGKDLFEF
                     DYSIMTPTLWIALVVGFIVAFIVSIIVMEKFVNFLKKKPMRVFAVYRIIMGVVLAVLA
                     FTNIISV"
     gene            complement(3953..4726)
                     /locus_tag="ACGI59_002023"
     CDS             complement(3953..4726)
                     /locus_tag="ACGI59_002023"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFG9739809.1"
                     /translation="MYLGSSFLGRNISVILKQQHKLYQSMIQEHKKIHSEHLTFINQW
                     IHQMKTPISVISLQLQDYEGEEVTENIRKEIDRLNKGLDMAMHFARADNFQKDFIVEK
                     AKLNEIVSSIVNEEKRLFIKSGMIPKIQVDKFTYVYTDVKWMKFIIGQLITNGVKYSK
                     DYSNYLTIKSEENNKYVILSIIDEGIGIVSKDLKRVFDPFFTGENGRKFGESTGMGLY
                     LVKKVCDDLGHKVSIKSEVGKGTEVSIYFNKDVNSLIKV"
     gene            complement(4967..5659)
                     /locus_tag="ACGI59_002024"
     CDS             complement(4967..5659)
                     /locus_tag="ACGI59_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFG9739810.1"
                     /translation="MSYKVYIVEDDISISTLLKDYIEKYGFEVIVEENFDDIMITFES
                     FKPHLVLLDVNLPKFDGFYWCRCIRQQSNLPIIFISARDGNVDQIRALESGADDYITK
                     PFYYDVVMAKIKSHIRRVYGEYSPKIDERIVELDGLKFYPERLELELNGENLIITKKE
                     GILIECLIKRYPKVVSRDYLLEKIWDDIEFVEENTLNVNVSRIRKRFEELGIDNAVQT
                     VRGLGYKLNKNW"
     gene            complement(5974..6933)
                     /locus_tag="ACGI59_002025"
     CDS             complement(5974..6933)
                     /locus_tag="ACGI59_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFG9739811.1"
                     /translation="MKLKKLLIGITILTLATASLAGCAKKNNGEKLSEINLTYVKSPL
                     NVPSIIQKQDDLFGKEFKKDNIKVNFHEITTGPEQTQALAAGEIDFLHALGGTSALIA
                     ASNGVELKILNTYSRSPKGFMILTNNNSIKSAADLVGKKVAGPKGTILHQVLISALDK
                     EGLSMDDVEFVNMGIPEASAALSDGSVDAALIAGPAALKAMKSGSKLVANGEGLVDGI
                     IVTAVSTDFAEKHPEIVERFMKVEKETLEYVNNNFDEAMEKVAKEVDLSLEETKELYA
                     WYDFSLDITDKDISSLEDTQDFLIKNKLQEKKVNIKELIYNAK"
     gene            complement(6966..7844)
                     /locus_tag="ACGI59_002026"
     CDS             complement(6966..7844)
                     /locus_tag="ACGI59_002026"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9739812.1"
                     /translation="MNNLVCDSGYIIRNLYKSYKVDNEEHVVLSDISLNIDRNHITVI
                     LGESGCGKTTLLRAIASLEGINSGEIRFINDNKEYRPNVGFVFQESRLMPWLNVSENI
                     AFHNQRKNTILNKVLRKFKHNKRISSKIINDNFRSLETNDNLNKSNKYNRKSNIDVEK
                     YLKMMNLQKFKNSYPNELSGGMAQRVSIARALSFNPDMLLMDEPFSALDYFTRIDMQN
                     EVIRIHKSTNKGVIFVTHDIDEALKIGKKIVVFTDERKVKEFNIEDGYNRDLTSDYYI
                     KLKKDILMTMKSNKGI"
     gene            complement(7837..8619)
                     /locus_tag="ACGI59_002027"
     CDS             complement(7837..8619)
                     /locus_tag="ACGI59_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9739813.1"
                     /translation="MVNKFNNKFLRLVKSVLIFFIILLLWKITNYLGIWSDYILPSPE
                     KVYSTFLNMISDGSIFINVYASMKRVLIGFAISTAIGVPLGIFFGIYSGVYEYFKSLI
                     NFLRNTPPLALIPMLILWFGIGEESKIIIIVLASFFPIFTSTLKGIKNCDSKLIEVGR
                     VFEFSKLQIIFKIIIPNAILDIAVGLKLALGYSFRAIIGAELVAASSGLGYLISDGKE
                     MSRTDVVIVGIIVIGLLGIITDYIFSIIVKKVSKGKMVEAYE"
     gene            complement(8620..9249)
                     /locus_tag="ACGI59_002028"
     CDS             complement(8620..9249)
                     /locus_tag="ACGI59_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9739814.1"
                     /translation="MNKYLLINPVAERMYGEKFHLIKANLIKKGYIIAECEPQLEYVK
                     KQYKEYTKKTENTMLDCRCPESISLLKRNNLINDFEVPMIEPILVRTCRVLYDKYIKS
                     KDDMLVVTCPCTQLRDFASEKFKDKGNAMFYTWKEFAEQEGVKSLGKIDESPIPLGYF
                     KDTFEKVLEVSSEDEILSEIQKIRNGFEDKYDIVEMFYCKDGCNNGDGL"
     gene            complement(9644..10270)
                     /locus_tag="ACGI59_002029"
     CDS             complement(9644..10270)
                     /locus_tag="ACGI59_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFG9739815.1"
                     /translation="MKYEWRKRDKELYLPKKNPVIVEIPEQKFIMLKGSGDPNSKEFT
                     EAIGVLYSLAYAIKTMPKKGIVPEGYFDYTVFPLEGIWDKGEKSKGLDVLIKEDLIYT
                     IMIRQPDFVTQDLLDKATDIVKKKKPHNLLEKVRFGSINEGLCVQMLHIGSYDNEPES
                     FDIMNEFCKKNKLIRKSYTHREIYISDARKVSPESLKTVLRFEVEREV"
     gene            complement(10346..11317)
                     /gene="nrdF"
                     /locus_tag="ACGI59_002030"
     CDS             complement(10346..11317)
                     /gene="nrdF"
                     /locus_tag="ACGI59_002030"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFG9739816.1"
                     /translation="MTFNLHKIHNAVNWNREEDGFTQAFWEQNVKQFWLPEEISVSKD
                     IKVWNELSNKEKELYKKVLGGLTLLDTKQGNNGIPSMMSLTENLQRKAVLSFMGTMEE
                     IHAKSYSSIFMTLLSNLEIDELFEWIETEPTLQRKADLVLAQYENTTNQEGLYLSMVT
                     SVFLESFLFYSGFFYPLYLSGQGKMVASGEIISLILRDESLHGKYIGLLAQEIYDSFD
                     KMDKEMLEEKMYSILYSLMENEIEYTNVIYRESGLEKEVVNFLKYNANRALENLGFEK
                     LYTVDAINPIVLNGLSTETKTHDFFSTKGNGYQKGVYEELEDEDFII"
     gene            complement(11330..13429)
                     /gene="nrdE"
                     /locus_tag="ACGI59_002031"
     CDS             complement(11330..13429)
                     /gene="nrdE"
                     /locus_tag="ACGI59_002031"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFG9739817.1"
                     /translation="MNWIELNNQVIIKNEQGKYQLEKDKEALSSYIEEFIQPKLRKFN
                     NLEERLKYLIDEGYYSKEVINQYSIDVIKNIYDMIDKHKFEFQSYMSANKFYQNYALK
                     SNDGKEILETYNDKVLIVALTLGNGDKDLALNLADKIVKQEFQPATPTFLNAGRKRAG
                     EMVSCFLLSVEDSTEGISYAISSSNHLSKIGGGVALNLSKLRASGESIKDIEGAAGGV
                     VGVAKMLEQSFSYFNQMGARQGSGAVYLTVFHPDFELLMDTKKINADEKIRLATLSLG
                     AIIPDKFMELAEKNEVAYAFYPHTVYKKYGVSLDEIEMDKWYDKLVNDSDIRKKEINP
                     RQMLTKIAQMQQESGYPYVVYIDTANREHTLKDVGIIKMSNLCCEIFQYQTPSEIEGY
                     GGKNEWGQDISCNLGSLNIANVMDNKTIESTVETAIRALSFVADSTDIKPVPTVSNSN
                     SKSHSIGLGAMNLHGYLVRENILYTSDDAIDFSNVFFAMVRYYSIKASMKIAIERNQT
                     FEGFDKSEYVKGRNSKVLSKYYEQSYLPKSEKVRALFEGIYIPTKEDWTKLLDEVKEK
                     GIYNAYLMAVAPTQSISYVQNATSSIMPITEPVEVRTYGDSTTIYPMPFLTNDNMLYY
                     QSAYRMDMRKVIDLVATVQNHVDQGISTTLFVTDEKTTRDIARHYIYAYKKGLKSLYY
                     TRTKMTRDTHECLVCSV"
     gene            13907..14632
                     /locus_tag="ACGI59_002032"
     CDS             13907..14632
                     /locus_tag="ACGI59_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFG9739818.1"
                     /translation="MKTISKQELEKTLEKHYLWLKNNNAGEKADLKELRLENMDLRGY
                     CLSNIDFSWSDLINLNLEKADLENSIFNNSYLSDCSLKNSILKNADLSGASFRFCDLS
                     NSDIRGANLENSNLEYATLNGVISDESTRFFKLYCPETGAFIGYKKCFNFRMVQLLIP
                     SDAKRVSATSNACRCDKAKVLSITSVDGKESFKSARAYADENFIYRVGETIVAEDFNE
                     NRWKESTTGIHFFLTREEAIGYL"
     gene            complement(14760..15956)
                     /locus_tag="ACGI59_002033"
     CDS             complement(14760..15956)
                     /locus_tag="ACGI59_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9739819.1"
                     /translation="MLKTNNLSVGYNNKVVISNINVEVKNGEILCLLGSNGAGKTTLL
                     RSLSKLISPIKGEIYLNGVNINCISRKALSKKMALVLTNRLLGDLMTVQDIVNIGRYP
                     YTGFFGSLSKKDLIMVDEALESVDALHLKKRYFDELSDGEKQKVLVARALVQEPEIII
                     LDEPTTHLDIKHRLELINILKKLSKEKSISVILSLHEIDIALKSCDKVALIKNNKVIA
                     YGQPEDVVDEDIINSLYELDDKNFNSLLGSVEISNKSKNEVFIIGGGGKATPIYRAFT
                     KKGIGLYSGIIHENDIDYEIGRTMGIKMFTENPFEPISDESFDLAIRNLNDSKIIIDT
                     GFSVGETNKRNIDIIKEALKLDKKVYSFRNRDESKKYYDSLDSKIEHIDKVSQIINSA
                     DINNLL"
     gene            complement(15959..16984)
                     /locus_tag="ACGI59_002034"
     CDS             complement(15959..16984)
                     /locus_tag="ACGI59_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFG9739820.1"
                     /translation="MDFINKMRIRNTTIFIVLIISMILTFLVCVAFGSVKIEFKEVID
                     VVLRGMDNTEHYKIIYNIRIPRVLATMVGGACLAVSGILLQIFFKNPIVEPYILGVSS
                     GSTLSVALMILGGYTLGFESVNSMSIFVAAFIGALIVMSIVILFANKVKNITTLLIIG
                     IMTGYVCSGITNVLQSFANSDKLKEFTMWSMGSFSGFTWEKVEILIIVGILGLLMTVF
                     IIKPLNAFLLGEEYAKSIGINIRFFRVLIVFISSILAAIVSAFAGPIGFIGLAVPQIS
                     KMIFKTSDNRVLIPVVIFLGSIVTALCDLISRVILSPVELPISTITSLLGAPIVVMLL
                     VKRKDRA"
     gene            complement(17056..18186)
                     /locus_tag="ACGI59_002035"
     CDS             complement(17056..18186)
                     /locus_tag="ACGI59_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFG9739821.1"
                     /translation="MKFKSNVLKQFFLIAMVIFMVGGLIGCSKSDKENKQASTGKKLE
                     LKYASGFSVEDLEDGIKKVTDGDNRELILIPKKIKIPEKYKNANIVRTPVDNVLIAST
                     TQACSLRTIDELDSIKAVTTQEQYWTIKEIKDLMKDGKIHYVGSNLAPDYEKIVDLNP
                     DLTIVSSGLSEADTKFIDKLKELGLNYVVDNEYKEQNPFGRMEWTSLIAAFYDKEDMA
                     TSELNKTVQKVEEVSKKIKNKAKPKVVWASVYEGSAYIAPKDSYVDNMIKMAGGINAC
                     ASIDSNEGRVSIEQLHEVAKDADIFVYSSSSDYAPNLDSIKSSAPVLADLDVVKNGNL
                     WVFAPDYYQSVDKTDELIVDLVEMFHPGTTGEKIKHYINYDK"
     regulatory      complement(18414..18595)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(18648..19541)
                     /locus_tag="ACGI59_002036"
     CDS             complement(18648..19541)
                     /locus_tag="ACGI59_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HFG9739822.1"
                     /translation="MKAEFLTPVVTIFDEEGNLDKEGNKKVYDHLIDGGVDGLVIMGS
                     TGEFFNMSMDMQKELIDLVTSHVNKRVKVFIGTSRMCINESIELSNYAHNSGADGVMI
                     ISPYYFSLDDDSIELFYSEIAKNTEAEIYLYNYPDRTGYDLSPELTLRLARKHKNIVG
                     YKDTVTLMGHTRDLINTMKIEFPEFKVYSGYDENFIHNLMSGGAGCIGGLSNLIPEVC
                     SKWAEAYNNRDMEKVIEIQKYINKAMDFYLITNPFIPAMKKAMNIRGLQISDYMTAPF
                     VKPNEKQVCEIKKLMNELNIC"
     gene            complement(19621..20523)
                     /locus_tag="ACGI59_002037"
     CDS             complement(19621..20523)
                     /locus_tag="ACGI59_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFG9739823.1"
                     /translation="MKFVSFTEGENECKRTGVFSKDELFIIDVNSLNLSRNFKDLNEL
                     IQNVSSEDIIKLKTIINESTFYNYDVFKRERCTVHVPIEKPIHDILCVGVNYKDHLEE
                     TQTHFDDSFEEPQKTVYFTKRVTKAIGPEDEIDGHFEINNQLDYEVELGVVIGKTGVN
                     IRKEEVEDYIFGYTVINDISARKLQKEHVQWFRGKGLDTFTSLGPCILHKSAVPFPIK
                     LKVCSRVNGEERQSSNTGLFISDVSDIVSELSKGMTLEAGDIIATGTPSGVGMGFSPP
                     RYMSSGDVVECEIESIGILKNYIK"
     gene            complement(20573..21733)
                     /locus_tag="ACGI59_002038"
     CDS             complement(20573..21733)
                     /locus_tag="ACGI59_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HFG9739824.1"
                     /translation="MKFYGYKRPDGRVGIRNHILILPTSVCASDTTRIIASQVQGAVT
                     FNNQNGCSQVHSDQQLTMDVMAGYAANPNVYGVIVVSLGCENCQNDLVVDAIKERTNK
                     PIKTLVIQEEHGTLKTIEKAVRYAREMAQEASLLRKEEFPISELILGTECGGSDPTSG
                     LAANPLIGELSDKLVDLGATSILSETTEFIGAEHILARRAVNEEVKEKILHIVHRYEN
                     SLKLVGEEVREGNPSPGNIAGGLTSLEEKSLGCIHKGGHRQISEVYDYAKQIDKKGLV
                     IMDTPGNDASSVAGMVAGGAQIVVFSTGRGTPSGNPIAPVIKITGNKITFANMEDNLD
                     FDASPVIYGPQTMEELTDDLLNMVVDVANGKQSKAESLGYTEMAIARVCNYV"
     gene            complement(21889..22194)
                     /locus_tag="ACGI59_002039"
     CDS             complement(21889..22194)
                     /locus_tag="ACGI59_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HFG9739825.1"
                     /translation="MINAVIIDEKDNVAVAIEAIAKGSEISFKLKDKTVKTITALDDI
                     TIYHKLAIKDMAQGDKVTKYGEHIGEANQEIKSGQHVHIHNVRSVREDLDKQVMTNI"
     gene            complement(22292..23266)
                     /locus_tag="ACGI59_002040"
     CDS             complement(22292..23266)
                     /locus_tag="ACGI59_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HFG9739826.1"
                     /translation="MIMDFIKKIPAGMMIVPMFIAAFINTFCPDIVQIGSFTTAVFSS
                     VGSATIIGVQLVCLGTQLQFREMPKVLKRGGILLGSKFAIGALIGILIGKLFGMDGVL
                     GLTTLAVISAVTNSNGSIYLSLMTSYGDETDCATMSLLTLNDGPFFTLIALGTSGLAN
                     VPLLSLLAAIVPILVGMIIGNFDKKMKEFLEPGCNLLVPFVGFALGSSINLASILKGG
                     LSGVLLGLISVFVGGIFIVFCDKFIGKRPGYAGWAVATTAGNAIAVPAAVALVDPSWQ
                     PYVATATTQVAAAVVVTAILVPLITSWWAKKYGCPKFPKQNEDGTITA"
BASE COUNT         7772 a         4249 c         2422 g         9176 t
ORIGIN      
        1 aaacatctat aattccagta atattaatta attttaaacc ctttttatta tcatttaata
       61 atagtaacta aaaattgact aaatttaacc ttatctcttt taaataatta tttcttacaa
      121 taaaaaaata aactatttga accaagaaca agcttcccat taccaaaaca gctgatgatg
      181 ctacttctat atcaaaagaa tttttaagtg cagtaagtgc aaatattgaa tgtataacag
      241 ccactacata aggaaacaaa aatactattc caatctcaat tgttgaagct tttttaatct
      301 cattatatgt catacccaat ttatttaact gttcatactt tttcttatca acttggcaat
      361 ccatataaaa tttattatat aaaaaactac ttgtagtaac aaagaaaatt aaacctataa
      421 atattgctaa aaatataaat gtagaataca ttaatttccc agttcctatc attaattctt
      481 tagagaaaaa caaatatggt tgcatccctg actcattatt aaatttattt tcaaattctc
      541 tgcatatctc tatagtttta cttgaatcct ctacttcaaa tgcagtaaat ctatccacta
      601 taaattttga ttctatacta tcataaaaat tatcctttac cacatacata tttttgtaat
      661 atgctggcat aacacattct tccacttgtt ttgccacctt taaaccccta ttccctacaa
      721 taatattttc tttgacgtta ttatttcctg gtaaacttga tgataataat gatatacatt
      781 cattatcttc aaaacttata gacttaaccc ctatagtttc tgtaatttct aagtacttag
      841 attctttcat aattctgaca ttaaaatcac ttttcttggg aaatacagtt ttaaattcac
      901 cattagtcct tgtatagtct acattctctt tttttagatt attttctata aacatcattc
      961 tttccttgtc tttttctttg ctatcaggct ttttagggtc attatgcaat gtcattgttg
     1021 aataatatat tgtgttagga tatattaaat taatttgacg ctctacatct ttccaaaagg
     1081 aataaactgt acctatagca gtaaatgcta ctgcagaagt tatagttatc aagaaaaata
     1141 ttctagcatt atcctttacc ttataaatca agttcgaaat ccaaagcata tttgtatttt
     1201 ttctataaaa tctcttgttt agttttagct tcttcataaa aaatacacta aattgagaaa
     1261 agaacaagta agttgctact atagtaaggg atgtaactgg aactaagcgc agatatgttg
     1321 tatattggtc ggttgaggta atagcattat aatacgctat tataaacaaa caaatactta
     1381 atatagcaag cattagtgat gttttaggct ctggttttgg agtttttgta cctttaagta
     1441 acttcaacac ctgattttct ttaactataa aagaagtaaa tattgatgtt aatacagcta
     1501 aaatcataaa atagattaaa gtagacaata tcgcattaaa tggtatatag aattttaatg
     1561 gtgataattc taaaaaagta gacataatca ctaaaaatat ctttgcaaag attaagccta
     1621 ttattattcc aattacagaa gatactatat ttattatcat attttctata aagattaatt
     1681 tcattacttg gcgctttgat atacctgtaa tataaagtat tccaaattct ttatatctac
     1741 ttttaagaaa cacactaact gaataaaaca caaagaagaa taagaataga tatgcaatta
     1801 tcttactcat cttgagacct tcttttaaat aatcaggaaa ttgagataca tctaaatctg
     1861 gatgacttgt aaacataata aaagaaaata gcagtgaaga tgatataaca atacttgcta
     1921 agtatcctaa atacgcttta atatttcttg aaatattgtt aatagcaaac tgttttatat
     1981 tcattagtta tcgcctccca ataatgtaag tacatccatt atctcttgat aaaactgctc
     2041 tctactacta cccttatata tttcattata tatagaccca tcttttatga ataagattct
     2101 actacagtag ctcgctgcta atgggtcatg agttaccatc atagtagtaa ctttattttc
     2161 tttatttatc ttttgaaata gctccattac aacctttgat gactttgaat ctaaattacc
     2221 tgttggttca tctgctaaaa gtattgatgg attgttaatc aatgctctag caatagcagt
     2281 cctctgagct tgaccacctg atacttcaaa agttcttttt cctaataaat cttttatacc
     2341 taatattgta gatacttcat tcaatctctc atcttgctct tttacactga ccttatctaa
     2401 tgtcaatggc aatactatat tttctcctat agttaatgta tccaataaat taaaatcctg
     2461 aaatacaaaa cctaactctc ttcttctaag taatgcaagt tcctcacctc ttaacaataa
     2521 aggattcttg ccctttaatt ccatagttcc tgtagttggc ttgtcaatag tagaaatcat
     2581 atttaaaaga gtagtttttc cactaccaga tggacccata attcctacaa attctccatc
     2641 ttctatacta aaatttatgt catttaatgc gttgaaaatt atttttttac catatacttt
     2701 acttaaatta tttacagtta aaattttcat tatttaacta tccttcctaa tttaattttt
     2761 gaaataacat atatataatt gttaattata atttatctta gtttaatttt tatagatgat
     2821 aaatctattt tttctataca ctaattatgt tagtaaaagc taatactgca agtactacac
     2881 ccattattat tctgtataca gcaaacactc tcattggttt tttctttaaa aaattcacga
     2941 atttttccat aactattatt gatacaataa aagctactat aaacccaact accaaagcaa
     3001 tccacaatgt tggtgtcatt attgaataat caaattcaaa taaatctttt cctgaagaag
     3061 ccaccatagc tggtactgct aaaaagaaag aaaactcagc tgctgtagga gaattaagtc
     3121 ctgctatcca tccacccata atagtagatg cactccttga catacctggc catgcagaca
     3181 aaacttgtaa tgtcccaacc tttaaagctt gtattggagt aattttatct atattatctg
     3241 tggaatgatt cttttttcta aacaaagttt ctactactaa taataaaatc ccacccacta
     3301 taaatcctat aattacagat tgtaaattaa acaatgattt tatattatcg taaaatagaa
     3361 ctcctataat cgcaaatggt atacaaccta taattacatt cattccaaat ctaaaaccaa
     3421 tctttccttc ttttccacca gtaaatataa atttaaaaaa ttctataacg ctatctttta
     3481 tctttttcca ataaagtact actactgcta atatagcccc aagttgtatt acaacctcaa
     3541 acatttcagc aaattgcccc ttaaaatcta ttaaattacc aaccaaaatc atatgacctg
     3601 tagatgaaac tggaataaat tctgtaagcc cctctactat agctattatg actgatttca
     3661 atataaaaat aacatctaaa ctcatcaata aacatccttc cttttttaca aaatttcttt
     3721 ttttatgtta tttctcttat gttatttctt atattaattg taatttagat tagaaaattt
     3781 aactatctgc aaacattaca caaaccttac atctatgtaa ggtttgcaaa taaacgctct
     3841 taaattttga ttatttaact atgaatattt catttaataa attgtataat aaaaaagtac
     3901 aattaactaa aaaccctatt gtaacagata atttacatct ttatcaaact atttacacct
     3961 ttattaaact atttacatct ttattaaagt agattgaaac ttctgtacct tttcctactt
     4021 ctgattttat acttacttta tgacctaaat catcgcatac cttttttact agataaagac
     4081 ccatacctgt agattctcca aacttacgac cattttctcc tgtaaaaaat gggtcaaata
     4141 ccctttttaa atctttagaa acaattccaa ttccttcatc tattatactt aaaattacat
     4201 atttattatt ttcttcagat tttatagtta ggtagttact ataatcttta gaatatttca
     4261 ctccatttgt aattaattga cctattataa atttcatcca ttttacatct gtataaacat
     4321 aagtaaattt gtctacttga atcttaggaa tcattccact ttttataaat aacctttttt
     4381 cttcatttac aatactagat acaatttcat ttaatttggc tttttcaact ataaagtctt
     4441 tctgaaaatt atctgccctt gcaaaatgca tagccatatc taacccttta tttaatctat
     4501 ctatttcttt tcttatattt tcagttactt cttctccctc ataatcttga agctgaaggc
     4561 ttataactga tattggtgtt ttcatctgat gaatccattg gttgatgaaa gttaaatgtt
     4621 cactatgtat ctttttatgc tcttgtatca ttgattgata caatttatgt tgttgtttta
     4681 aaataactga tatatttctc cccaagaatg agcttcctaa atacaaaaat gactcctcca
     4741 aagaatgtac tcctttttca aacagattat atacttctct atttttataa tacttatata
     4801 ttaaaaaaca taacaatata aaagaactaa ataaaagaat atataaactc tctccaaaac
     4861 taataaactt cataagatta cagtaaataa ttgtcataac tatgcctata taatatataa
     4921 aaatataacc tttataatct tttaaaaata acttcatact ttctccttac caattcttat
     4981 ttaatttata tcctaaccct cggacagtct gaacagcatt atctatacct aattcctcaa
     5041 atcttttcct tatcctgcta acatttacat ttaaagtatt ttcctctaca aactcaatat
     5101 catcccatat cttttctaaa agataatctc tacttactac ttttgggtat ctcttgataa
     5161 gacattcaat cagtatacct tcttttttag ttataattag attctcacca tttaattcta
     5221 gttctaacct ctctggataa aactttaatc catctagctc cactattctt tcgtctattt
     5281 taggtgaata ttcaccatat acccttctta tatgactctt tatttttgcc attactacat
     5341 catagtaaaa aggttttgtt atataatcat ctgctccact ttctaaagct ctaatttggt
     5401 ctacattacc atctctagct gatataaaaa ttataggtaa attcgactgt tgtctaatac
     5461 atctacacca gtagaaacca tcaaatttag gtaaatttac atctaataga actaagtgtg
     5521 gcttaaaact ttcaaatgta atcattatat catcaaagtt ttcttcaaca atcacttcaa
     5581 atccatattt ctcaatataa tctttaagta atgtacttat tgatatatca tcttccacaa
     5641 tatatacttt ataactcata taattcctcc taatatacct atttactcat catattaaca
     5701 gtccaatttt aatatattat aatcttttta caatatataa atttaatcat attactaact
     5761 tcacaattac tgacttcata agtataacca tattattaga catcttaata aaactctata
     5821 aaaaagtatc tttttatgat taattaatca ttattatcaa ctaatcattc taaaataaat
     5881 tgaaattact aattcaaaat agaaaaagtt accttaataa ggtaacttaa acttttacta
     5941 tatttcttac ttagtattat tttatcatag tttctactta gcattataga ttaattcttt
     6001 tatgttcact tttttctctt gtaacttatt ttttattaag aagtcttgtg tatcttcaag
     6061 cgaagatatg tctttatctg ttatatctaa actaaaatca taccaagcat ataattcttt
     6121 agtttcttca agacttaaat caacttcttt agctactttt tccatagctt catcaaaatt
     6181 attatttaca tattctaaag tttccttttc aaccttcata aatctttcaa ctatttctgg
     6241 atgtttctct gcaaagtctg tgctaacagc agttactatt ataccatcta ctaaaccttc
     6301 accatttgct acaagtttac tacctgactt catagcttta agagctgctg gaccagcaat
     6361 caatgcagca tctacgctgc catcagaaag agctgctgat gcctctggaa tacccatatt
     6421 aacaaattcc acatcatcca tacttaatcc ttctttatca agtgctgaaa tcaatacctg
     6481 atgtaatata gtcccttttg ggcctgccac cttcttacct actaaatctg ctgctgattt
     6541 tattgaatta ttattagtca aaatcataaa tccttttggt gacctgctat aagtgtttaa
     6601 tatctttaac tctactccat tagatgcagc tattaaagct gatgtaccac ctagtgcatg
     6661 taagaagtct atttctccag cagctaaagc ttgtgtttgc tctggacctg tagtaatttc
     6721 gtggaaatta acttttatat tatccttttt aaattccttt ccaaataaat catcctgttt
     6781 ttgaattata gaaggaacat ttaatggtga ttttacataa gttaaattta tttctgaaag
     6841 tttttctcca ttattttttt tagcacatcc agctaaagat gctgttgcaa gtgttaatat
     6901 tgttatacct atcaatagct tttttaattt catttacttt actccttaaa atattattaa
     6961 tttctttaaa tgcctttatt tgatttcatt gtcattaaaa tatctttttt taatttaata
     7021 tagtaatctg aagttaaatc tctattataa ccatcttcta tattaaattc ttttactttt
     7081 ctttcgtctg tgaaaacaac tattttcttg cctatcttta acgcttcatc aatatcatga
     7141 gttacaaata tcacgccttt attagttgac ttatgtattc ttataacttc attttgcata
     7201 tcaatccttg taaaataatc taatgccgaa aaaggctcgt ccataagcaa catatctgga
     7261 ttgaaagaaa gagctctagc tatgcttact ctctgcgcca tacctcctga caattcattt
     7321 ggataagagt ttttaaattt ctgcaagttc atcatcttaa gatatttttc tacatcaata
     7381 ttacttttcc tattatattt atttgatttg ttcaaattat catttgtttc taagcttcta
     7441 aaattatcat ttataatttt actgctaatt cttttattgt gtttaaattt tctaagcacc
     7501 ttgtttaaaa tagtattttt tctttgatta tgaaaagcta tattttcact tacgtttaac
     7561 caaggcatta atctgctctc ctgaaaaaca aaaccaacat taggtctata ctctttatta
     7621 tcattaataa acctaatttc accactattt ataccttcaa gtgatgcaat tgctctaagc
     7681 aaggtagtct ttccacaacc actttcacct aaaattactg ttatatggtt tctatctatg
     7741 tttaaagaga tatcactaag aactacatgt tcttcattgt caactttata acttttatat
     7801 aaatttctaa ttatatatcc agagtcacat actaaattat tcatatgctt ccaccatctt
     7861 tcccttactt accttcttca caataatcga aaatatgtaa tctgtaatta ttcctaaaag
     7921 tccaattacg attataccta caataacaac atctgttcta gacatttctt ttccatcaga
     7981 tatcaagtat ccaagaccag atgaagctgc tacaagttct gccccaataa tggctctaaa
     8041 gctatatcca agagctaact ttaacccaac agcaatatca agaatggcat ttggtattat
     8101 tattttaaat attatctgaa gctttgaaaa ctcaaatact ctacccactt caatcagttt
     8161 tgagtcacaa tttttaatcc ctttcaatgt gcttgtaaag attgggaaaa aggatgctaa
     8221 aactataatt ataatttttg attcctctcc tataccaaac cataatataa gcattggtat
     8281 taaagcaagg ggaggtgtat tccttaaaaa atttattaga gatttaaagt attcgtatac
     8341 tccactataa attccaaaga atatccctaa aggcacacct atagcagtac ttattgcaaa
     8401 acctatcaaa actcttttca tacttgcata tacattaata aatattgaac catcacttat
     8461 catatttaaa aatgtactat ataccttttc aggtgatggt aatatataat cactccaaat
     8521 accaagataa tttgtaattt tccataataa aagaattata aaaaatatca aaacactctt
     8581 tactagcctt aaaaacttat tattaaattt attgaccatt tacaaaccat ctccattatt
     8641 acaaccatct ttacaataaa acatttctac tatgtcatac ttatcttcaa agccatttct
     8701 tattttttgt atttcactta atatttcgtc ttcacttgat acttcaagaa ctttttcaaa
     8761 agtatctttg aaatatccta atggaattgg tgattcatct atttttccaa gtgattttac
     8821 accttcttgt tcagcaaact ctttccatgt ataaaacata gcattaccct tatccttaaa
     8881 cttttcactt gcaaagtctc ttaattgtgt acatggacag gttactacca acatatcatc
     8941 tttacttttt atatatttat cgtataaaac cctacaagtc cttactaata ttggttctat
     9001 cataggtact tcaaaatcat ttatcaaatt atttcttttt aaaagactta tactttctgg
     9061 acatctacaa tccaacattg tattttcagt cttttttgta tactctttgt actgcttttt
     9121 tacatattct aactgtggtt cacattctgc tattatgtac ccctttttta tcaaatttgc
     9181 tttaataagg tgaaactttt ctccatacat tctttctgcc actggattta ttagaagata
     9241 tttattcata acgcctccta ttatttaata ttgaatatta ttatcaatta atagtttaaa
     9301 tgagcactta aattttgtca atatattaat tttttcttaa taaaacaatt tttatctaca
     9361 ttttttaata ttttaaatga aatattaaat agtatatgta atttatttta attaataatg
     9421 catacatctt actaataatc tatttatata ataatctata aatattatat aataatactt
     9481 atataattag tattattatt taatagcaac agttattcac actaataaaa gtagaaagtt
     9541 tttaaaatat tatgaaacat caaatttaat tttaaaattg catcaaaaaa tgaagtctca
     9601 acagacttca ctttttataa ctcttataaa ttattcaaaa tatttatact tccctttcta
     9661 cttcaaaacg taaaaccgtc ttcaatgatt caggcgatac ttttctagca tcacttatat
     9721 aaatttcacg atgtgtgtaa gattttctta taagtttatt ttttttgcaa aattcgttca
     9781 taatgtcaaa gctttctggt tcattatcat atgaacctat atgaagcatt tgaacacata
     9841 gcccttcatt aatagaccca aatctaactt tttcaagaag attatgaggt tttttcttct
     9901 ttactatatc agtagcttta tccaacaggt cttgtgtaac aaaatctggc tgacgtatca
     9961 ttatagtgta aattaaatct tctttaataa gtacatctaa gcctttactt ttttctccct
    10021 tatcccatat accttctaaa ggaaaaaccg tatagtcaaa atacccttct ggtacaattc
    10081 cttttttagg catggttttt attgcatatg ccaaagaata aagtactcct attgcttcag
    10141 taaattcttt agaatttggg tctccactcc cttttaacat gataaatttc tgttctggta
    10201 tttcaactat tacaggattc tttttaggta aatataactc tttatctctt tttctccatt
    10261 catatttcat aaaaatacct ccatatatat tttatacatt gatattatca cgctaaaagt
    10321 gacaacagtg tgtcataatt ataaattata ttataaaatc ttcatcctct aattcctcat
    10381 acactccttt ttggtatcca ttacccttag ttgaaaaaaa atcatgtgtt ttggtttcag
    10441 tgctaagacc atttaataca attggattta tagcatctac agtatatagc ttctcaaacc
    10501 ctagattttc taaagctcta ttcgcattgt attttaaaaa gttcacaacc tctttttcaa
    10561 gcccactttc tctgtatata acatttgtat actctatctc attctccata aggctataaa
    10621 gtatagaata catcttctct tctaacatct ccttatccat tttatcaaat gaatcgtata
    10681 tttcttgtgc tagcaatcct atatacttac catgtaaaga ctcatctcta agaataagcg
    10741 atattatttc tccactagct accatctttc cttgaccaga tagatacaat ggataaaaga
    10801 atcctgaata aaacaagaaa ctctctaaaa atacacttgt aaccattgat aggtataatc
    10861 cctcttgatt tgtagtattt tcgtattgag ctaaaactaa atcagccttt ctttgtaatg
    10921 ttggttctgt ttctatccat tcaaataatt catctatttc taaattagaa agtagagtca
    10981 taaatataga agaataactt tttgcatgta tttcttccat agtccccata aaagacaaga
    11041 cagcttttct ttgtaggttt tctgttaaac tcatcataga tggtattcca ttattcccct
    11101 gttttgtatc cagtaatgtt aaaccaccca atactttttt atataattcc ttttctttat
    11161 tacttagttc attccatacc tttatgtctt tagaaacaga tatttcttca ggaagccaaa
    11221 attgttttac attttgttcc caaaaagctt gtgtaaatcc atcttcttct ctattccagt
    11281 taactgcatt atgtatttta tgtaaattaa atgtcatata tttctctccc tatactgaac
    11341 acactagaca ctcatgagta tctcttgtca tctttgttct tgtgtaatat aaacttttca
    11401 aacctttttt ataggcatat atgtaatgtc tagctatatc tctagttgtc ttttcatctg
    11461 taacaaacag tgttgttgat ataccttggt caacatggtt ttgaactgtt gccactaaat
    11521 ctatgacctt tctcatatcc atcctgtatg ctgattgata atacaacata ttatcatttg
    11581 ttaaaaatgg cattggatat attgtagtag agtctccata tgttcttact tctactggct
    11641 cagtaattgg cattatactt gatgtagcat tttgaacata actaatgctt tgagttggtg
    11701 ctactgccat taaatatgca ttatatatcc ctttttcttt tacctcatcc aataattttg
    11761 tccagtcttc ttttgttggt atataaatcc cttcaaataa tgctcttacc ttttctgact
    11821 taggtagata agattgttca tagtattttg ataaaacctt gctatttctt cccttaacat
    11881 attctgattt atcaaatcct tcaaaagttt gatttctttc tatagctatt ttcatagaag
    11941 ctttaattga atagtatcta accattgcaa aaaatacatt agaaaaatct atagcatcat
    12001 ctgaagtata taaaatattt tctcttacta agtatccatg taaattcata gctcctaatc
    12061 ctatagaatg agacttgctg ttactattgc ttaccgttgg cactggtttt atatctgtgc
    12121 tatctgctac aaatgacagt gctcttattg ctgtttcaac tgtactttct atagttttat
    12181 tatccattac attggctatg tttaaagaac ctaggttaca acttatatct tgtccccatt
    12241 cattttttcc tccatacccc tctatttctg aaggtgtttg atattgaaat atctcacaac
    12301 ataagttaga cattttaata attccaacat cttttaatgt atgttctcta ttagctgtat
    12361 ctatgtacac tacatatgga tatccacttt cttgttgcat ctgtgctatt tttgtcaaca
    12421 tctgcctagg atttatttct ttttttctta tatcactatc attaaccagt ttatcatacc
    12481 acttatccat ttctatctca tcaagagaaa ctccatattt cttatacaca gtatgaggat
    12541 aaaatgcata tgctacttca tttttttctg ccaactccat aaatttatct ggtattatag
    12601 ctcctaatga taatgtagca agccttattt tttcatctgc atttatcttt ttagtatcca
    12661 tcaacaattc aaaatcagga tggaatacag taaggtacac cgcaccagaa ccttgtctag
    12721 ctcccatttg attaaagtaa ctaaatgatt gttctaacat tttagctact ccaacaacac
    12781 ctccagctgc accttctata tctttgatag attcgccaga tgctctaagt ttagataaat
    12841 ttagtgcaac tcctccacca atctttgata agtgatttga tgaagatata gcatacgata
    12901 taccttctgt actatcttct acagatagta aaaaacatga taccatttct ccagcccttt
    12961 ttcttccagc atttaaaaaa gtaggtgtag ctggttgaaa ttcttgtttt actattttat
    13021 ctgccaagtt taaagctaaa tctttatcgc catttcctag tgtcaaagct acaattaaca
    13081 ctttatcatt atatgtttct aatatttcct ttccatcatt gctctttaat gcataatttt
    13141 gataaaactt atttgcactc atatatgatt gaaattcaaa cttatgttta tcaatcatat
    13201 catatatatt tttaataaca tctatgctat actgatttat tacttctttt gaataataac
    13261 cttcatctat taaatacttc aatctttctt ctaggttatt aaattttctc aactttggct
    13321 gtataaattc ttctatgtaa ctacttaagg cttccttatc cttttccaat tggtacttgc
    13381 cttgttcatt tttaattatc acttgattgt taagctctat ccaattcatt atttaccgtc
    13441 cttttaacat aatcttgtgc cataatttca cttaatttgc ttattatttc ttttggtact
    13501 tatcagttga aatcccaaaa ttaatctata attatagaaa tgcaataagt atttttcaaa
    13561 aacaaaagca ctacatatag tcagtgcttt atctattata tactacatat agtatataca
    13621 caaatttttt tatatctaaa cttttattac tttataattg acattatttt tatttaaaga
    13681 aatttttatc aactcaaata taaaacaata caagcttatt atgtcctgtt taaaaattta
    13741 aattatctct aaatagctta aaaatatatt ctgatatatt gaaaaaagta ctttcaagat
    13801 gatattttat atattattta gaagtttttt atttattgtt atataattaa atatatgact
    13861 attttgctac tacatatata aaaataaatt tatgaaggtg attttaatga aaactatttc
    13921 taaacaagaa ttagaaaaaa cacttgagaa acattattta tggcttaaaa acaataatgc
    13981 tggagaaaag gcagatttaa aagaattaag attagaaaat atggacttaa gaggatactg
    14041 cttaagtaat attgactttt cgtggtctga ccttatcaat ttaaatttag aaaaagcaga
    14101 tttagaaaac tctattttta ataattctta tctttcggat tgttcattaa aaaattctat
    14161 attaaaaaat gcagacttaa gtggtgcaag ttttagattt tgtgacttat ctaactcaga
    14221 tattagagga gctaatttag aaaattccaa tttagaatat gctactttaa atggagtaat
    14281 atcagatgaa agtactcgtt tttttaaatt gtattgtcct gaaacaggag catttatagg
    14341 ctataaaaaa tgttttaatt ttagaatggt acaattatta atacccagcg acgcaaaaag
    14401 agtgtctgct acatcaaatg cttgtagatg tgataaggca aaagtactgt caattactag
    14461 tgtagatggt aaagaatctt ttaaaagtgc tcgtgcatat gctgatgaaa attttatata
    14521 tagagtagga gaaacgattg tagctgaaga ttttaatgaa aatagatgga aagaatcgac
    14581 tacagggatt cacttttttt taactagaga ggaagctatt ggttatctat aaaatgctaa
    14641 agttttataa atatataaat acataaacaa aataacaaaa gagtatagat tgtaatttca
    14701 aaaaaattat aatctatact cttttttcta taacacataa gtacatttta tttcataaat
    14761 tataataaat tatttatatc tgcactattg attatttgag aaactttatc tatatgttct
    14821 atcttactat ccaaactgtc ataatacttc ttactctcat ctctatttct aaaagaataa
    14881 acttttttat ccaattttaa agcttctttt ataatatcta tattcctctt atttgtctct
    14941 cctacactaa atcctgtatc tattataatc ttggaatcat ttaaatttct aatagccaaa
    15001 tcgaaactct catcacttat aggctcaaat ggattttctg taaacatctt tatccccata
    15061 gttctaccaa tttcataatc aatatcattt tcatgtatta ttccagaata cagacctata
    15121 ccttttttag taaaagctct ataaattggt gttgctttac ccccaccacc aattataaaa
    15181 acttcatttt tagatttatt actaatttct actgaaccta aaagactatt aaagttttta
    15241 tcatccaatt cataaagcga atttataata tcctcatcta ctacatcttc aggctggcca
    15301 taagctatga ctttattatt ttttataaga gcaactttat cacaactttt taatgctatg
    15361 tctatttcat gcaatgaaag tattactgat attgactttt ctttacttaa ttttttcagt
    15421 atgtttatga gttccaatct atgttttata tccaagtgag ttgtaggctc atctaatata
    15481 attatttctg gttcttgaac aagagccctt gcaaccaaca ccttttgttt ttctccatca
    15541 cttagttcat caaaatatct tttctttaaa tgtaaggcat ctacagactc caatgcttca
    15601 tctaccatta ttaaatcttt cttagataga ctcccaaaaa atcctgtata tggatacctt
    15661 cctatattga ctatatcttg tacagtcatt aaatcaccta ataatctatt tgtaagtact
    15721 aaagccattt tttttgataa tgcttttctt gatatacaat ttatgtttac accattcaaa
    15781 tatatttcac cttttattgg tgatataagt ttagataagg accttaatag ggtagttttt
    15841 ccagctccat tacttcccaa aagacatagt atttctccat tttttacttc tacatttata
    15901 ttacttataa caactttgtt attgtatcct acacttaaat tattagtttt caacatgtct
    15961 aagccctatc cttccttttc accaataaca ttactacaat tggagctcct aataaagatg
    16021 ttattgtact tattggtaat tctactggtg ataaaattac tcttgatatt aaatcacaaa
    16081 gagctgtaac tatactgcct aaaaatatta ctactggtat caaaactcta ttatctgatg
    16141 ttttaaatat catttttgaa atttgtggca ctgcaagtcc aataaaccct ataggtcctg
    16201 caaatgctga tactattgct gctaatatac tagatataaa tactatcaaa actctaaaaa
    16261 atcttatatt tatgccaata ctttttgcat actcttctcc tagtaaaaaa gcatttaaag
    16321 gttttattat gaatacagtc atcaaaagac ctaatatacc aactattata agtatttcta
    16381 ccttttccca tgtaaatcca gaaaaacttc ccatagacca catagtaaat tcttttaatt
    16441 tatcactatt tgcaaatgat tgtaacacat ttgttatacc actacataca tatccggtca
    16501 taattcctat tattaaaaga gttgttatat tctttacctt atttgcaaat aaaatgacta
    16561 tagacattac aattaaagct cctataaaag ctgctacaaa tatactcatt gaatttacac
    16621 tctcaaatcc caatgtgtat cctcccaaaa tcatgagagc tactgataaa gtagaacctg
    16681 aagatactcc taatatatac ggttcaacta ttgggttttt aaaaaatatc tgtaataaaa
    16741 tacctgatac tgctaggcat gctccaccta ccatggttgc aagtactcta gggattctta
    16801 tattgtatat tattttataa tgttctgtat tatccatacc tcttaataca acatctatta
    16861 cttctttaaa ttcaattttt acagagccaa atgctacaca cactaaaaaa gtaagtatca
    16921 tacttataat taatactata aatattgtcg tattccttat tctcatttta ttgataaaat
    16981 ccatttgtat actcctttta gcaaagctat attaactaaa atctataaaa cctatctttt
    17041 tgtgtcctct ctgtactatt tatcataatt tatataatgt ttgatttttt cacctgtagt
    17101 acctgggtgg aacatctcaa ctaaatctac tattaattcg tctgtcttat ctactgattg
    17161 ataataatct ggagcaaaca cccataggtt cccgtttttt acaacatcca aatcagccaa
    17221 tacaggtgca ctagatttta tagaatctaa atttggtgca taatctgaac ttgatgagta
    17281 tacaaatata tctgcatctt ttgcaacttc atgcaattgt tcaatactta ctcgcccttc
    17341 atttgaatct attgaagcac atgcatttat tccaccagcc atttttatca tattatccac
    17401 atatgaatct ttaggagcta tataagcact tccttcatat acagatgccc atacaacttt
    17461 aggtttagct ttgtttttta tttttttact tacttcttct accttttgta cagttttatt
    17521 tagttcacta gtagccatat cttctttatc ataaaatgca gctattaaac ttgtccactc
    17581 cattcttcca aatggatttt gctctttata ctcattgtct acaacataat taagccctaa
    17641 ttcttttaat ttgtcaataa attttgtatc agcctctgat aaaccactac tcactatagt
    17701 taagtcagga tttaagtcta ctattttttc ataatctggt gctaaatttg aaccaacgta
    17761 atgtattttt ccatctttca ttaaatcttt aatttcttta atagtccagt actgctcttg
    17821 tgttgttacg gcttttatac tatctagttc atctattgtt cttagagaac aagcctgtgt
    17881 tgtagatgct attaatacat tgtccacagg cgttctaact atattagcat ttttatattt
    17941 ttcaggaatt ttaatcttct ttgggattaa aatcagttct cgattatctc catcagttac
    18001 ttttttaatt ccatcttcta aatcttcaac actaaatcca ctagcatatt ttaattctaa
    18061 tttcttacca gtactagctt gtttattctc tttgtcactt ttactacacc ctataagacc
    18121 tcctaccata aaaattacca tagcaattaa aaaaaactgc tttaaaacat ttgatttaaa
    18181 tttcatctct ttcactccaa tatcttattt tttatgagtg gctaaaaaac tttaataata
    18241 tatgcttttt aaagtatact tattaaagtt atcctaaagt catctttaat aagtataacc
    18301 ttttaataaa ataaaaaact tctcctaatt taataaggag aagttcatat atcaacttta
    18361 caaaaacgaa ttgatacttc attttattat aaattacaat ctccacccta aattgaactt
    18421 atataatact gggcaggtct cctgactcac ggcttttcct ccatcaccct tcccaaaatt
    18481 tttataaatt tcagtggatt ttgatttcgt atccgtatac agtagcgggg gctgtagtga
    18541 attttcatca ctttccctat taaactaata tagtacccta gtattatttt aatttaattt
    18601 ttattaagat aattgtaatt tattgttgac ctctttgcaa ggttgattta acatatattt
    18661 aattcattca tcaatttttt aatttcacat acttgttttt catttggttt tacaaatgga
    18721 gcagtcatat aatcacttat ttgtaaccct ctgatattca tagctttttt cattgctggt
    18781 ataaatggat tggttattaa gtaaaaatcc attgctttat taatatattt ttgaatttct
    18841 ataaccttct ccatatctct attattgtaa gcttcagccc atttggagca aacttctgga
    18901 attaaatttg ataacccccc tatacaacct gctcccccag acattagatt atgtataaag
    18961 ttttcatcat aaccactata aaccttaaat tctgggaact ctattttcat agtatttatt
    19021 aaatctctgg tatgtcccat caaagttact gtatccttat atccaactat gtttttatgt
    19081 tttcttgcaa gtcttaatgt aagttctgga cttaaatcat aaccagttct atctggataa
    19141 ttgtacaaat atatttcagc ctctgtattc ttagctattt cactataaaa taactcaata
    19201 ctatcatcat caagagagaa ataataaggg cttattatca taacaccatc tgctccacta
    19261 ttatgtgcat agtttgaaag ttctatagat tcatttatac acatacgact tgtacctata
    19321 aacactttta ctctcttatt tacatgacta gttactaaat ctataagttc tttttgcata
    19381 tccatagaca tattaaagaa ctctccagta cttcccatta tgactagtcc atctacacct
    19441 ccatctataa gatggtcata cactttttta ttaccttctt tatctaaatt tccttcttca
    19501 tcaaatattg taacaactgg tgtcaaaaat tctgctttca taatttaaac tcctaaaatt
    19561 tattttaata tgttcattaa tacatcagat ttagctttaa tattaattat attaaaacag
    19621 ttacttaatg taatttttta aaattccaat actctcaatc tcacattcaa ctacatctcc
    19681 actactcata tatcgaggag gtgaaaatcc catccctact ccagaaggtg ttccagtagc
    19741 tataatgtct ccagcctcta aagtcatacc ttttgaaagt tctgaaacta tgtctgaaac
    19801 gtctgatatg aataaccctg tattagaact ttgtctttct tcaccattta ccctactaca
    19861 tactttaagt tttatgggaa aaggaactgc acttttatgt aaaatacatg gacctaaaga
    19921 ggtaaatgta tctaatcctt ttcctctaaa ccattgaaca tgttcttttt gcaactttct
    19981 agcagaaata tcatttataa cagtgtaacc aaagatataa tcttctacct cttcttttct
    20041 aatattaaca cctgttttac ctattactac tccaagttca acttcatagt ctagttgatt
    20101 atttatctca aaatgaccat ctatctcatc ttctggacct attgcctttg tcacacgttt
    20161 tgtaaaataa accgtttttt gtggttcttc aaaactatca tcaaagtgag tttgagtttc
    20221 ttcaagatgg tctttatagt tcactcccac acataagata tcatgtattg gtttttctat
    20281 aggaacatgt acagtacatc tctctctttt aaaaacatca taattataga atgtactttc
    20341 atttataata gttttcaact taataatatc ttcactagat acattttgaa ttaattcatt
    20401 taaatcctta aaatttctac tgagatttaa agagttaaca tctataataa ataattcatc
    20461 tttagaaaaa actccagttc ttttacattc attttctcct tcagtaaaac ttacaaactt
    20521 cataatctca ccctttatta aaattctatg tatttatttt cttacttaaa gattatacat
    20581 agttgcatac tctagctatt gccatttcag tatatccaag ggactctgct tttgattgtt
    20641 ttccatttgc tacatctaca accatattta acaaatcatc tgttaattct tccattgttt
    20701 gtggaccata tataactgga ctagcatcaa aatccagatt atcttccata tttgcaaaag
    20761 ttattttatt tccagttatt tttattactg gtgctattgg atttcctgat ggtgttcctc
    20821 ttcctgtact aaataccact atttgtgctc ctcctgctac cataccagca acagatgaag
    20881 catcatttcc aggggtatcc attataacaa gacctttctt atcaatttgt ttggcataat
    20941 catatacttc acttatttgt ctatgaccac ctttatgtat acaaccaagt gatttttctt
    21001 ctaaacttgt taaaccacca gctatatttc caggagatgg attaccttct ctaacttctt
    21061 ctccaaccaa ttttaatgaa ttttcatatc tatgtactat atgtaatatt ttttctttta
    21121 cttcttcatt tactgctctc ctagctagta tatgctctgc acctataaat tcagtagttt
    21181 cacttaaaat tgatgttgct cctaaatcga caagtttatc acttaattca cctattaatg
    21241 gatttgctgc taatccactt gttgggtcag acccaccaca ttcagttcct agtataagtt
    21301 cagatattgg aaattcttct tttctaagca aagaagcttc ttgtgccatt tctctagcat
    21361 aacgaacagc tttttctata gtttttaaag ttccatgttc ttcttgtatt acaagagttt
    21421 ttattggctt atttgttctc tcttttattg catcaactac caaatcattt tggcaatttt
    21481 cgcatcccaa agatacaact attacaccat atacatttgg atttgctgca taaccagcca
    21541 taacatccat agttaattgt tggtctgaat gtacttgtga acatccattc tgattattaa
    21601 aggtaactgc cccttgaact tgtgatgcta ttattctagt tgtatctgat gcacacacac
    21661 tcgtaggcaa tattaatatg tggtttctta taccaactct tccatctggt cttttatatc
    21721 cataaaattt catactgatt tcctcctaaa atagtctata tactttctat aatttttata
    21781 tattataatt tttattacac tcactaatca aacagctaaa aacctattta aaaactattt
    21841 gacactaaaa tcaatgaata caacttttat aactctataa atattatttt atatattagt
    21901 cataacttgt ttatccaaat cctctctaac acttctaaca ttatgaatat gtacatgttg
    21961 accacttttt atctcctgat ttgcttctcc tatatgttca ccatatttag taactttatc
    22021 accttgggcc atatctttta tagccaattt atgataaata gttatatcat ctagtgctgt
    22081 aatcgttttt actgttttgt cttttaattt aaaactaatt tcactacctt tagctattgc
    22141 ttcaatagct actgcaacat tatctttttc atcaattatc actgcattta tcattattct
    22201 tgctctcctt tataaataat ataattttat taatatccta aaatctaatt aaagaattta
    22261 ttattttacc aacaactcac tgtaatataa attaagctgt tattgtacca tcctcatttt
    22321 gttttgggaa cttaggacat ccatacttct tagcccacca actagttata agaggtacta
    22381 atattgcagt gacaactact gcggctgcca cttgtgttgt agctgttgca acatatggtt
    22441 gccatgatgg gtcaactaat gcaacagcag caggtacagc aattgcattt ccagcagttg
    22501 tagctacagc ccatcctgca tatcctggac gttttcctat aaatttatca cagaatacta
    22561 taaatatacc tcctacaaat acagatatta atccaagtaa tactcctgat aaacctcctt
    22621 ttagtatact tgcaagattt atgctactac caagtgcaaa ccctacaaaa ggtacgagta
    22681 aattacaccc tggctctaaa aactctttca tttttttgtc aaaatttcct attatcatac
    22741 caacaagtat tggcactatt gcagccaata atgacaacaa tggtacattt gcaagacctg
    22801 aagtcccaag agctattaat gtaaaaaatg gtccatcatt taaagtaagt aaactcatag
    22861 ttgcacaatc tgtttcatct ccatatgaag tcatcaatga aagatatata ctaccattac
    22921 tatttgtaac tgcacttata acagccaatg ttgtaagacc aaggacacca tccattccaa
    22981 atagctttcc tataagtata cctatcaaag cacctatagc aaatttagaa ccaagaagta
    23041 taccacctct ctttaaaact tttggcattt ctctaaattg aagctgagtt cccaagcata
    23101 ccagttgaac cccaattata gttgcagacc caacacttga aaatactgct gtcgtaaacg
    23161 aacctatttg tacaatatct gggcaaaagg tattaatgaa agctgctata aacattggca
    23221 ctatcatcat acctgcagga atttttttaa taaaatccat tatcataaag ttaatcctcc
    23281 taaaacatga tatttaatta ctcttattaa gtttttagct ttaattctaa cggccgtttc
    23341 ggttcgtatt taagaactaa gtttttaatt acgaatcttt gaattaatga tagcattttc
    23401 ctatttatga gtcaataaaa aaaataaatt tttgtcaaaa ttttttcaaa ctttctgata
    23461 ataccttgta tattatcaaa tgacttcctt atatgcaaaa atacagcgta tttaaaatta
    23521 tatagtattc aaaactataa agtttttata cagtgtaaaa aatagataaa atataagttc
    23581 ataatatttt gatatttaca tataaaaata actattgca
//
LOCUS       DAYAPA010000056        23551 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.056, whole genome shotgun sequence.
ACCESSION   DAYAPA010000056 DAYAPA010000000
VERSION     DAYAPA010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23551
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.056"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            262..2280
                     /locus_tag="ACGI59_002041"
     CDS             262..2280
                     /locus_tag="ACGI59_002041"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFG9739827.1"
                     /translation="MRRNTKLLTTGILSMAIVAPTMAFATESNAMENNADLNINLEKK
                     SIVLGSTSKVSVKFKEKPDADSITLKYKCYDMPLDTTLNYNQSTESYEGTINYNKDPE
                     YLNVWELQGITINSKNNPKTLNKQELEKMGLNLKDYNVTQECIIEDITSRKDVNKYLR
                     KTSAPITELTGSDRYETAVKISKEGWKNGSDKVVIINGDVSIDGIISTPLATTYNAPI
                     LLVEKNNVPNSVKSELKRLNPRDVIIIGDENAISKTTANQIKSTVNASQTRLKGSNRY
                     ETSLLIAKEIDKNHDVEKVYITNANGGEVDALTIAAKAGQDKQPIILTDKNSITDNTY
                     KWLKSEDLQNAYFIGGPQMISTNVINKVNDITKDNVTNNRVYGADRHETNANVIKKFY
                     TDDELEAVLVAKSDVLVDALAAGPLAANLKSPILITPKTYVSAYHKENLEAKSANKVY
                     KIGGGLTSKVMSSIASSLSKHNTTPTEPGNSGGKTVMIDPGHGGSDTGTTGKPLGGIR
                     EKDYTLNTSLATTEYLRSKGFNVIMTRDTDKTLSLGNRTALSNSLRPDLFTSIHYNAS
                     DTTGNGVEVFYKLKDKDGGTTKTVATNILNRILEKFNLKNRGAKTRTLSTDPTKDYLY
                     VLRNNDMPAVLVECAFLDNEKDMSLLNTSNKVKEMGTQIGKGIEDSLK"
     gene            2785..4797
                     /locus_tag="ACGI59_002042"
     CDS             2785..4797
                     /locus_tag="ACGI59_002042"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFG9739828.1"
                     /translation="MRRNTKLLTTGILSMAIVAPTMAFATESNAMENNADLNINLEKK
                     SIVLGSKSKVSVKFKEKPDADSITLKYKCYDMPLNTTLNYNQSTGAYEGTINYNQDPE
                     YLNVWELQGITINSKNNPKTLNRQDLEKLGLNLKDYNVTQECIIEDITSRKDVNKYLR
                     KTSSPITELTGSDRYETAVKISKEGWKNGSDKVVIINGDVSIDGIISTPLATTYNAPI
                     LLVEKNNVPNSVKSELKRLNPKDIIIIGDENAISKTTANQIKSTVNASQTRLNGSNRY
                     ETSLLIAKEIDKNHDVEKVYITNANGGEVDALTIAAKAGQDKQPIILTDKDSITDNTY
                     KWLKSEDLQNAYFIGGPQMISTNVINKVNGITKDSVTNNRVYGADRHETNANVIKKFY
                     TDDELEAVLVAKSDVLVDALAAGPLAANLKSPILITPKTYVSAYHKDNLEAKSANKVY
                     KIGGGLTSKVMSSIASSLSKHNTTPTEPGNSGGKTVMIDPGHGGSAPGNSSGGMIEKD
                     YNLNTSLATTEYLRSKGFNVIMTRDTDKTLSLGNRTALSNSLKPDLFTSIHYNGSTNK
                     QGHGVEVFYKLKDKNGGTTKTVATNILNRILEKFKLTNRGIKTRVLPSDSTKDYLYVL
                     RSNDMPAVLVECAFLDNENDMSLINSSAKVKEMGTQIGKGIEDSLK"
     gene            5039..6952
                     /locus_tag="ACGI59_002043"
     CDS             5039..6952
                     /locus_tag="ACGI59_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9739829.1"
                     /translation="MEYLFEKSSLKEYGNEALDKYRELLDKTGNNSRCILLLVPNNNT
                     KIRYERSLRLNYSEELKITTYISFVKKELVKYWPLIIEKCDEISTKVVSPTFISNSLS
                     EYLIVQKVKEKRIQEGYFEDITGTNKSIANSIMTNINKSAFNLIDFDFIGEKIYSSKK
                     NKDSIYRFSYTQMDEIISYYINSLLSNSILDNALSIYLYNQYLLNDDFYLKNLFTEVR
                     YIIVDSLESSSNAEVDFITEALDNTLQSYIYFDYSRDYSVFNNVDIDYINEKIISKIK
                     PKEENIKKKEIRIEDLYLLPANIELNQSSQLYNEMLDLISEKVISLIEEDVSPRDIAI
                     ISPINNSILEHQIRDSLIEKNIDVFSTKKDKKAVDYPYGNALVVATCIFYGYLDFIKD
                     DEYLSFLETLLEINRIKAFKIFNETRHLSIDNIDEHSQYREILQYIEEKKKSDIKIHE
                     FLTQFYIDKMLNLKEGKKNVGLCKKIIAESESFSESLSLLGMKKEKIFIEALKSTIND
                     YYSVVDIEELKSKDKIVITTPYSYISSNIDRSIQIWVDIGSNAWNMKIEKDISNLIVL
                     RKSFEEKKIYTNEMEEYYKKYYLYNTVYNLLLNAKKIYAFKSEYAINGYIQESILYSI
                     LLKISHKGDKNYD"
     gene            6945..9164
                     /locus_tag="ACGI59_002044"
     CDS             6945..9164
                     /locus_tag="ACGI59_002044"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFG9739830.1"
                     /translation="MININYREDQIPIINYDNGTMAVPAVPGAGKTFIITNLVAKLLL
                     EQKHKGGKILILTYMNSAVNNFKGRIRKILEENKIDDTNSYEVMTIHSLAVKIIKEKP
                     EVVMLSEDFNIADDLQKSIILNECISRFRFEGGERAFRWFLKEQKDERWKEITLEAWE
                     RGFFEFAGNAISELKYKGISPDNLEEILSRGHKGMLKIILPIYKLYDRKLKQNGLLDY
                     DDILILAEKTLSLDEGLRKKFQTRYKYIFEDECQDSNEIQGNIIKIISSENKNLVRVG
                     DINQSITGTFSSSDPKFFKEFINSADFCYRMDMSNRSSKDILDLANTLVKYVTKEFRQ
                     KECRHALEDMQIKTVPNGMGYKENPNPEHYNINVKWYESWKIEIEQTARYVKGIKNKY
                     PDKSIGILVPFNEQVTQVAKELRENNLTFEELGPNSLNKRKVINNIAYIIDFMLHCDD
                     VEKLIVVLNKVFIDTDNEEEIKYFLEELKNYSTEEIIYNFEFSKLDNDLIEFKEESDI
                     YLSFKRGIDIIKQILEYPIIRLDLLVLFIGKKLNLEKEDKAVLDYISFYIKYLVSENI
                     NMDLEDVYNLLFDVKNRVFNHIIDVVYEINGYEPEPGSITLCNYHKSKGMEWDCVFLL
                     GLVEYNFPDNINQKFQSDKWYLKEKYKNPMAIIKSEVEAILKGSISTDYAHKTKIDSI
                     NEKIRLLYVGITRAKEMLVLSGSAYRDESDIGNKRKEQKPCIYLSRLNQHIIEKRSN"
     gene            9273..10103
                     /locus_tag="ACGI59_002045"
     CDS             9273..10103
                     /locus_tag="ACGI59_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HFG9739831.1"
                     /translation="MNDKLKYFSYSQNSINTYKSCPLKFKYKYIDRINWKNDDVGSRE
                     YYETLKTGRDFHLICERYFSNIPLGIYFNEDDKNSKKFLKWMENIKKVVPIEKGKTYL
                     PEYEVRMTLNGDIIQAKYDLVVIGNNNIEIWDWKTESKKIDYKHVENRIQTIVYMFLA
                     KEVIPKVLKMDINVKDINMRYYQPEFDDLPITILYDEEKHEANRNKIQNYISMIKNTN
                     YEEHTYEDNLYNDIEKVYNKEERMCYRNKKHCKYCEFNKLCNGKEIDYSILEAEIYGT
                     "
     gene            10093..13560
                     /gene="addB"
                     /locus_tag="ACGI59_002046"
     CDS             10093..13560
                     /gene="addB"
                     /locus_tag="ACGI59_002046"
                     /EC_number="3.1.-.-"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddB"
                     /protein_id="HFG9739832.1"
                     /translation="MGLRFVLGRSGSGKSTYILDEIKKEAQKNETTSIILLVPEQYTF
                     EAENRVSKLFLGKEKDKYLRVRVLSFKTLSNIVFSQVGGLTDVNINSSGKAMMVYRAI
                     EDVSEELNVFSKSKSQSGFVSSITDMISEMKQYNISPEMLENISGELDNETLSLKLKD
                     ISKIYNSFEGKLHENYVDAQDMLTSLASKIELSSYLDGACVYIDEFTGFTPNQYNVIK
                     SILNKSKSVNISLTVDDINYIGYSKSDMFSRTKFTYSKLTQLCNEEGIKILPQVNLNT
                     GVIKRFEKVKELQHLERFYNAYPYKIYSNPTENIKIKEFNNLYSEVEEIAREIVHLVR
                     DKNVRYRDITIATRDLNRYDFLVHSIFNEYNIPNFIDKKREAKSNPIVILIISALEMK
                     NRRYGYETMFRYLKSGLIGIDNDDINLLENYVLANGIKGKKWFDEKWDYRITQSLSGQ
                     ESEFELELKEKINEIKNRVLEPIVILQEKLRGKNRVKEICRYIYEFLLDINMPETIES
                     LIVNFKDKGELDVANQYSQVWDIVVDILDQMVELMGDEIISLEKFIKLITLGFDEYEL
                     GLVPPSIDQVLVSSVDRMKNPDTKYLYLVGTTDGVFPLITKDSGILSDNDRESLGNKG
                     IEVDIDSKTRSFEEQFLVYKALTSTSKNLTITYPISDHEGKTLRPSIIISRLKKIFPN
                     IENKSYLVEENKSTDKDILKKITVKSPTFNELINVIKNYDSDGYNTEEINSIWLDIYR
                     YYLKDEIYSSITKKVIKGLSYTNQVHKIEEKKIRSLYKSNSLSISRLEKYAECPFAYF
                     IQYGLKAKKRKEYSFTPPDLGTFIHNILDRFSKELLQDNLTWRDIDEKYIELKIGIIV
                     DEIILKIPGYILNSSERYKYLAYRLKNMLTTAITIISQQIKQGSFEPIDYEVKFGNNG
                     KYPPIKMVLENGQEVSLIGQIDRVDEFEEGENKYIRIIDYKSGNKSISLTEIYYGLQL
                     QLLVYLDAILESAKDEDININPAAILYCRINNPIAKFNEDKDDEEIQEAILKELRMKG
                     LVVKDSHIVKEMDKSLIDGERKNSLVIPVGLTKDGNVGKSTSAISYEDFKLLRKYVRH
                     AIKDLCEEMLSGEIRIAPYKHKDGTSCDFCDYSAICQFDSTIKDNKYKNLNNKSNEEI
                     IKMMKGDVN"
     gene            13560..17387
                     /gene="addA"
                     /locus_tag="ACGI59_002047"
     CDS             13560..17387
                     /gene="addA"
                     /locus_tag="ACGI59_002047"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HFG9739833.1"
                     /translation="MSSPKWTKEQLEVIESRECNLLVAAAAGSGKTAVLVERIIQMIT
                     SRENPIDIDKLLVVTFTNAAASEMRERIGDAIGKALDENPENKHLQNQLVLLNKSSIT
                     TIHSFCLDVIKSNFHRINLDPNFRIGDQTECAILKQEAIEEVFEDLYEERDEGFLNLV
                     ESYAERGGDKEVQDIILGIYSFAMASPEPKKWLIDSAERFNIDENFDFSQSIWARAIL
                     DTVKIEINGLCLNMERALKEVESIEELETFAEKLSVEYKKIADISQACNKSWDEAYKK
                     MASMSFENYVKGVKRISKDAPSYIKESKEKAKTIRDKTKKSLESIVSATFNKDNDSIR
                     EEIKYLYNIVKPISSVVLRFEEEYSNKKREKGIIDFNDIEHFALNILTDVDEKGNIVP
                     SDIAVGCRNKFYEIFIDEYQDSNLVQEVLLKAVANTETPNRFMVGDVKQSIYRFRQAK
                     PELFLQKYNNYNDKKGSSHRKIMLYKNFRSREEVVDAVNYIFENIMNENIGEIEYTEK
                     ERLNLGANFNVDTDEKSIIGGATEIHLIQKDNKLDDDIINDKDDRINNKENEIEEEEK
                     LDNIQLEARMVGNIIKDLMKVNEDGKIQKVYDKGIDGYRPVEFRDIVILLRATSAWAP
                     VFADELMNMDIPTYADVGVGYFDTIEIKTILSLLQIIDNPMQDIPLISVLKSPIFGFT
                     PEDLIDIRVQSKDKIFYEVLKSTAEYDGFTDSQNENESEFIPSEECINKSKDFLIKLK
                     EFKEKSMYMSTDEFIWYLYTRTGYYAYVGALPGGSQRQANLKVLFERAKQFEETSLKG
                     IFNFVNFIEKLKKSSSDMGSAKTLGENANVVRIMSIHKSKGLEFPVVICSAMGKNFNT
                     QDFKKSILYHHNLGYGPQFVDYERRISFPSIAKEALKSKINIENLSEEMRVLYVAFTR
                     AKEKLIITGSTRNIQDSIKRWSNGIESLDTISQYEILKGKNFLDWIMPCVLRHRDLSN
                     LLEEVGLDAVFNVEHNSKWYGKLWNKNDILVEKKSDEEKESIEEILEKIDVNNPDSDY
                     YGEIEEKLNYIYPYEFSTRKPATISVTEIKKIQNNYEEELINTIFEQKVILKKPLFIQ
                     NEEEREKISGTERGTIVHLVMEVLDLKNVSSVNDIKSQIRGFVSKGIITEKQASIVNP
                     YKIYKFFASNIGKRMLNAEIINREKSIYAQVNMKDIYIYEKLINNDDKKLYDNESVML
                     RGIVDAYFEEDNQIVLVDYKTDFVNEENINQIIEKYKKQLDLYADIIETLTGKSVKEK
                     CIYLFGVDEAVCY"
     gene            17433..18644
                     /locus_tag="ACGI59_002048"
     CDS             17433..18644
                     /locus_tag="ACGI59_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFG9739834.1"
                     /translation="MRFIHTSDWHLGKSLEGHSRIEEQAKFCEEFIKIVESNEIDMVI
                     IAGDVYDTSNPPAQAEKLFYQTVSRLANNGQRCVLIISGNHDNPERLSAITPLAHEQG
                     ILIYGYPLSATIEAKYKGFEITYATQGCTKLNINGENIVVATLPYPSEKRLNEVFSSE
                     DEFEKQKNYSEKVGDIFRSLEENFRSDTINIAVSHIFVIGGESTESERPIQLGGSFLV
                     ERKDLPEKAQYTALGHLHKQQKASERLNAYYSGSPLQYSKDERAYTKGAYIVDIKAGE
                     KPIIEDVYFNNYKPIEVFKCNGIEEALDICEQNQDREIWSYFEINTDEIISQNEIKKM
                     KELLKDIIEIKPIITSCYEQESVDIKEKSMAELFREFYSFSKGVEPKGELMDLFLDII
                     SEEGESADETN"
     gene            18631..22158
                     /locus_tag="ACGI59_002049"
     CDS             18631..22158
                     /locus_tag="ACGI59_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFG9739835.1"
                     /translation="MRPIRLELTGLNSYIDKQVIDFEKLIERGLFGIFGTTGSGKSTI
                     LDAITIAMYGNISRNTKEYINSVCDKAIISYEFEIGSKNTRRRYIVDRTIARSKTGTK
                     TSNARLIEVLNDNTQNVLADKVVEVNEKVAQVVGLTANDFTRSVVLPQDKFNEFLRLS
                     RADRRDMLERIFNLEKYGRSLGEKVKKRKNIQLQNLKDLKSKLSQYDGITEEVYNNIN
                     QELIELKNLEKDKDNALDLAQKSYEESKTVYEEQLKLEKNELRKNELDLKNSEIKEKI
                     ALVENDSNAKKVDPYISSVQNLEKKIDEDSFAVSNLEKKLAILNQELEVTKNRYEKIN
                     KIKNEEVPKLSEEKIRLQQAIKLEEELVLLDRELKELKESGINLNKTKVELEKVKQVS
                     ESRKDAVTKSIKEVEGKIDKVNISAELKQKIFLAYEYEKDYNNVLEEKNQKLNKLEEI
                     LKETENINLKVRYIDKDKNDVNRNLENLSLHLDVLLKKCPGKSADLLLKSEYVTELRN
                     KANNTKENEIKKSSIQDELKIILESKFNTEREVNLLNEKLENNRKNRDDLEKELEELK
                     YLNLASELRRELKENMPCPVCGSKHHENHDITKYDENISFVKEKLEKLEKEKISIRNN
                     IEELNAKVSGYLSIEKMKTKELEDVKGKLGGIPSSQLLKKLDEEQRKLALLKSNIQEW
                     EKEKESTENKVTLAKEEKNKIEKEELKIRESLNNYKKLTRDLNIEIEGLENKCKKLKQ
                     EYLGLKTITKVSNLLSKVKEIRENEKELELLSTGHSNLLKNRDSLDIKIRENESKLHE
                     IEIELIKARELYVEKKLSRDNKYKEVISITKGDLAKNLLHNVEENICKILEQEESSKK
                     KLEEQRLEYEKNIAEKHNIDGRLKTAKEQYKDQKSTLNKLLAENKFESIYAVKRALLD
                     TDAVKKLIEEILEHEEEQKLLSFKIKSSKEKLNGRSIKKEYFEQLKDEIYNLKVEIGN
                     ISKDIGANQNQLITLKDSLDKINDFNKQLKVVEHNVDLLEELDKCVQGNKFVEYVSTN
                     QLKYIALEASKRLDGITKGRYALEIDSTLNFVMRDNFNGGERRSIDTLSGGETFLTSL
                     SLALALSSQIQLKGSAPLEFFFLDEGFGSLDSELLEIVIESLERLHSNNLSVGIISHV
                     EELKNRVPVKLLVSSSEAGIGSKVKIEYS"
     gene            22289..23542
                     /locus_tag="ACGI59_002050"
     CDS             22289..23542
                     /locus_tag="ACGI59_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HFG9739836.1"
                     /translation="MLESPNNLIQIIFLIVLLIGSAFFSASETALMSLSKIRIRYMQD
                     EGVKGAKLVSSLIENPNKLLSSILVGNNVVNIAATSISTSLFIGLMGEKGVALATAVM
                     TVLVLIFGEITPKTIAANNSEKVSLLVSKPIKAIIFILRPIVWIFNIITNIIFKLFGI
                     TNKGAKSFITEEELKTMVNVSHEEGVLEMEEREIINNVFEFGDMQAKNAMVQRIDMVA
                     IDMEDSYDEIIQVFKTEKLSRMPVYEETIDDIVGILNIKDIIFLSDEEIESFDIKNYM
                     REPFFTYEFKKITQLLEEMKLEKSQMAIVVDEYGGTSGLLTIEDLVEVIVGDIEDEYD
                     EEEDEIQVIKEDEYIVDGSTKIGDVNELIGVNLESEEFDSIGGFIIGHLSRLPEENEV
                     IEVDNIRFCIESIEKNRIKKIRIYT"
BASE COUNT        10156 a         2279 c         4142 g         6974 t
ORIGIN      
        1 ttgtattaaa tttaataaac ctacaattgt tatatgtttt tatttgtctt ttatagattt
       61 ttttggcaat aaatgatata gtgaagataa ttaaatatat tcaatatatt taattttttt
      121 attttttgtt tttataattt ttttagaagt ttatttttta tgtacttatt tataattttg
      181 ggtaaaaaca ctttgatata atttcaaaaa ttaaaaggtt aaaaaacaaa gtagaaattt
      241 atgttaaata aggagattaa tatgagaaga aatacaaaat tattaacaac agggattctt
      301 tcaatggcaa tcgtcgcacc tacaatggca tttgctactg aatctaatgc tatggaaaat
      361 aacgctgatt taaatataaa cttagagaaa aaaagtatcg ttttaggtag tacctcaaaa
      421 gtcagtgtta agtttaaaga aaaaccagat gcagatagca ttacattaaa gtataaatgc
      481 tatgatatgc cattggatac aactttaaac tataatcaat caactgagtc atatgaagga
      541 actatcaatt ataataaaga cccagaatat ctaaatgttt gggaactaca agggataaca
      601 ataaacagca aaaataatcc taaaacttta aacaaacaag aactagaaaa gatgggatta
      661 aatttaaaag actataatgt aacgcaggaa tgtataattg aagatataac ttctagaaaa
      721 gatgtaaata aatatttaag aaaaacttct gcacctatta cagaacttac aggaagtgat
      781 agatacgaaa cagcagttaa aataagtaaa gaaggctgga aaaatggttc agataaggta
      841 gttataataa atggggatgt aagtatagat ggcattatat caactccact agcaactaca
      901 tataatgcgc caatactttt ggttgaaaaa aacaatgtac ctaatagtgt aaaatcagaa
      961 ttaaagcgtt taaatcctag agatgtaatt ataattggag atgagaatgc tatttctaaa
     1021 actactgcta atcaaattaa atcgactgta aatgctagtc aaacacgtct aaagggctct
     1081 aatagatatg agacatcttt attgatagca aaggaaatag ataaaaatca tgatgtggaa
     1141 aaagtataca taacaaatgc taatggcgga gaagtggatg cacttactat agcagcaaaa
     1201 gcaggtcaag acaagcaacc aattatatta actgataaaa atagtattac agacaacaca
     1261 tataaatggt taaagagtga ggatttacaa aatgcttatt ttataggtgg ccctcaaatg
     1321 atatcaacaa atgttataaa taaggtaaat gatataacta aagataatgt tactaataat
     1381 agagtatatg gagcagatag acacgaaaca aatgcaaacg taataaaaaa attctataca
     1441 gatgatgagt tagaggctgt tttagtagct aaatcagatg tacttgttga tgctttagca
     1501 gcaggtccat tggctgcgaa cttaaaatct ccaatactta taacaccaaa gacatatgta
     1561 tctgcatacc ataaagaaaa cttagaagct aaatcggcaa ataaggtata taaaataggt
     1621 ggaggattga cttctaaggt aatgagctct atagcatcat cattatctaa acacaataca
     1681 actccaacag aaccaggaaa tagtgggggc aagacagtta tgattgaccc aggacatggt
     1741 ggttcggaca ctggaacaac aggtaaacca ttagggggta ttagagaaaa agactataca
     1801 ttaaatactt cacttgcgac aactgaatat ttacgttcaa agggattcaa tgtaataatg
     1861 acaagagaca cagataagac tttatctctt ggaaatagaa ctgctttatc taactctttg
     1921 agaccagatt tatttacgag tatacactat aatgcatctg atacaactgg gaatggtgta
     1981 gaagtatttt ataagcttaa agataaagat ggagggacta ctaaaactgt agctacaaat
     2041 atattaaata gaatcttaga gaaatttaat ctaaaaaata gaggtgcaaa aacaagaaca
     2101 ctatcaactg accctacaaa agattattta tatgttttaa gaaataatga tatgccagct
     2161 gtacttgtag aatgtgcatt tttggataat gagaaagata tgagtttact taatacatca
     2221 aataaagtaa aagaaatggg tacacaaata ggtaaaggca tagaagattc attaaaatag
     2281 tctacaatga gagcatagaa aaatttaaaa taaattaatc tgcaatttta aatttaaact
     2341 aaagctataa gataagaatg taattataat agtaataaag actgtatata cattgtgcat
     2401 ataagtgcga aatgaggtat atacagtctt tttatgatta attgtaaaag gatttatatt
     2461 atatgaagaa tttacatatc ttaaggctac agttaattgt caagagttgg agtattcatt
     2521 tgtaatacac aataaaagct taattactat attttattgt gtgtttgtgt gttttttgtg
     2581 gatttttatg tcaataaatg atatagtata gataattaaa tgtattaaat atatttaatt
     2641 tttttatttt ttgagtttta taagtttatt tttatatatt acttatatat tatttatatt
     2701 ttgggttaga tacactttga catattttca aaagttaaaa gattaaaaat aaagtagaaa
     2761 tttatgttaa gtaaggagat taatatgaga agaaatacaa aattattaac aacagggatt
     2821 ctttcaatgg caatcgtcgc acctacaatg gcatttgcta ctgaatctaa tgctatggaa
     2881 aataacgctg atttaaatat aaacttagag aaaaaaagta tcgttttagg tagcaaatca
     2941 aaagttagtg tcaaatttaa agaaaaacca gatgcagata gcattacatt aaagtataaa
     3001 tgctatgaca tgccattgaa tacaactcta aattacaatc aatcaactgg ggcatatgaa
     3061 ggaactatca attataacca agacccagaa tatctaaatg tttgggaact acaagggata
     3121 acaataaaca gcaaaaataa tcctaaaact ttaaacagac aagacctaga aaagctggga
     3181 ttaaatttaa aagactataa tgtaacacag gaatgtataa ttgaagatat aacttctaga
     3241 aaagatgtaa ataaatattt gagaaaaact tcttcaccta ttacagaact tacaggaagt
     3301 gatagatatg aaacagcagt taaaataagt aaagagggct ggaaaaatgg ttcagataag
     3361 gtagttataa taaatgggga tgtaagtata gatggcatta tatcaactcc actggcaacc
     3421 acatataatg caccaatact tttggttgaa aaaaacaatg tacctaatag tgtaaaatca
     3481 gaattaaagc gcctaaaccc taaagatata attataattg gagatgagaa tgctatttct
     3541 aaaactactg ctaatcaaat taaatcaact gtaaatgcta gtcaaacacg tttaaatggt
     3601 tctaatagat atgagacatc tttattgata gcaaaggaaa tagataaaaa tcatgatgtg
     3661 gaaaaagtat acataacaaa tgctaatggc ggagaagtgg atgcacttac tatagcagca
     3721 aaagcaggtc aagacaagca accaattata ttaactgata aagatagtat tacagacaat
     3781 acatataaat ggttaaagag tgaggattta caaaatgctt attttatagg tggtcctcaa
     3841 atgatatcaa caaatgttat aaataaggta aatggaataa ctaaagatag tgttactaat
     3901 aatagagtat acggagcaga tagacacgaa acaaatgcaa acgtaataaa aaaattctat
     3961 acagatgatg agttagaggc tgttttagta gctaaatcag atgtacttgt tgatgcttta
     4021 gcagcaggtc cattggctgc gaacctaaag tctccaatac ttataacacc aaagacgtat
     4081 gtatctgcat accataaaga taatttagaa gctaaatcag ctaataaggt atacaaaata
     4141 ggaggaggat tgacttctaa ggtaatgagc tctatagcat catcattatc taaacacaat
     4201 acgactccaa cagaaccagg aaatagtggg ggcaagacag ttatgattga cccagggcat
     4261 ggtggttcag cacctggaaa ttcatctgga ggaatgattg aaaaagatta caatttaaat
     4321 acttcacttg caacaactga atatttacgt tcaaagggat tcaatgtaat aatgacaaga
     4381 gacacagata agactttatc tcttggaaat agaactgctc tatctaattc attgaaacca
     4441 gatttattta caagtataca ttataatggc tcaactaata aacaaggtca tggtgtagaa
     4501 gtattttata agcttaaaga taaaaatgga gggactacta aaactgtagc taccaatata
     4561 ttaaatagaa ttttagagaa atttaaactt acaaatagag gtataaaaac aagagtactt
     4621 cctagtgatt ctacaaaaga ttatttatac gttttaagaa gtaatgatat gccagctgta
     4681 cttgtagaat gtgcattttt ggataatgaa aatgatatga gtttaataaa ctcatctgca
     4741 aaagtaaaag aaatgggtac acaaataggt aaaggaatag aagattcatt aaaataaatg
     4801 gaaataaaaa tatttaaaaa cttaatgtaa atttatagtt gtaaatttat actaaagtca
     4861 aaaaatgaaa tataagtgta tcaatagtaa gactgtatgt acatttgcac gtgtgaatgc
     4921 aagtatatat acagtctttt tttgtagtta aatgcaataa tttgaatgtg ttttgtaaaa
     4981 tacacaatat gatataatta gataatatac acttaattta attgaggaag gtgattatat
     5041 ggaatattta tttgaaaaaa gtagtttaaa agaatatgga aatgaagctt tagataaata
     5101 tagagaatta ttagataaaa ctggtaataa ttcaaggtgt attcttttgt tagtaccaaa
     5161 caataacaca aagatacgat atgaaaggtc tctaagatta aattatagtg aagaactgaa
     5221 aataacaaca tatataagtt ttgtgaagaa agaattagtt aaatactggc cactaataat
     5281 agaaaaatgt gatgaaataa gtacaaaggt tgtatctcca acatttatat caaatagttt
     5341 aagtgaatat ttaatagtgc aaaaagttaa ggaaaaaagg atacaagaag gatattttga
     5401 agatattaca ggaaccaata aatccatagc aaatagtata atgactaaca taaataagtc
     5461 tgcatttaat ttaattgatt ttgattttat tggagaaaaa atatattcat caaagaaaaa
     5521 taaagatagt atatatagat tttcatacac tcaaatggat gaaattatat catattatat
     5581 aaattcctta ctatcaaatt caattttaga taatgcatta agcatatatt tatacaatca
     5641 atacctactt aatgatgatt tttatttaaa aaacttattt acagaagtga gatatataat
     5701 tgtggatagc cttgaaagct caagtaatgc agaagttgat tttataacag aagcattgga
     5761 taatacatta caaagttata tatattttga ttatagtagg gattattctg tatttaataa
     5821 tgtggatata gactatataa atgagaaaat aatatcaaaa attaagccta aagaagaaaa
     5881 tattaaaaag aaggagatta gaatagaaga tttatactta ttacctgcaa atatagaatt
     5941 aaatcaatca agtcaattat ataatgaaat gttagattta ataagtgaaa aagttatatc
     6001 tcttatagaa gaagatgtat ctccaagaga tatagcaatt atatctccta taaacaatag
     6061 tatattggag catcaaatta gagattcttt gatagaaaaa aatatagatg tttttagtac
     6121 taagaaggat aaaaaagctg tggattatcc atatgggaat gcattggtag tggctacatg
     6181 tatattttat ggatatttag attttataaa agatgatgaa tacttaagct ttttagaaac
     6241 tcttttagaa ataaatagga taaaagcatt taagattttt aatgaaacaa gacatttaag
     6301 tattgataat atagatgaac atagtcagta tcgtgagatt ttacagtata ttgaagaaaa
     6361 aaagaagagt gatataaaaa tacatgaatt tttaactcaa ttttacatag ataaaatgtt
     6421 aaatttaaaa gaaggtaaaa agaatgttgg gttatgtaaa aagataatag ctgaaagtga
     6481 gtcttttagt gaaagtttaa gtctacttgg gatgaaaaaa gaaaagatat ttatagaagc
     6541 tttaaaaagt acaataaacg attattactc tgttgtcgat atagaagagt tgaaatccaa
     6601 agacaaaata gtaataacta ctccttattc atatatatca tcaaatatag atagaagtat
     6661 acaaatatgg gtagatatag gaagtaatgc ttggaatatg aaaatagaaa aagatataag
     6721 taatttaatt gtacttagaa aatcttttga agaaaaaaag atttatacaa atgaaatgga
     6781 agaatattat aaaaaatact atctatacaa tacagtatat aatttgttat taaatgcaaa
     6841 gaaaatctat gcatttaaaa gtgaatatgc gattaatggt tatatccaag agagtatatt
     6901 atatagtata cttttaaaaa tctcacataa aggagataaa aattatgatt aatataaatt
     6961 atagagaaga ccagatacca ataataaatt acgataatgg tactatggca gtaccagcag
     7021 taccaggtgc tggaaaaaca tttataataa caaatttagt agctaaacta ttacttgagc
     7081 agaaacataa gggtggtaaa atattaattc ttacatatat gaatagtgct gtaaataatt
     7141 ttaaaggtag aataagaaaa attcttgaag agaataaaat agatgatact aattcgtatg
     7201 aagtaatgac tatacacagt ttggctgtaa aaataataaa ggaaaagcca gaagttgtaa
     7261 tgttaagtga ggattttaac atagctgacg atttgcaaaa aagtattata ttaaatgaat
     7321 gtataagtag atttagattt gaaggtggag aaagagcttt tagatggttt ttaaaggagc
     7381 aaaaagatga acgctggaaa gaaataactc ttgaagcctg ggaaagaggt ttttttgaat
     7441 ttgcaggaaa tgcaataagt gaacttaagt ataagggaat ttctccagat aaccttgaag
     7501 aaatattgtc aagaggacac aaaggaatgc ttaaaattat tttaccaata tataaattgt
     7561 atgatagaaa attaaagcaa aatggattgc tagattatga tgatatatta attttagcag
     7621 aaaaaacttt aagcttagac gaagggctta gaaagaagtt tcaaactaga tacaaatata
     7681 tatttgaaga tgagtgtcag gactcaaatg aaattcaagg taatataata aaaattatat
     7741 ctagcgaaaa taaaaaccta gttagagttg gagatataaa tcagagtata acaggaacat
     7801 tttcatcatc agaccctaaa ttttttaaag aatttataaa tagtgcagat ttctgttata
     7861 ggatggatat gtcaaatcga agttcaaaag atatacttga tttagcaaac actcttgtca
     7921 agtatgttac aaaggaattt agacagaaag aatgtagaca tgctcttgaa gatatgcaaa
     7981 taaaaactgt gccaaatgga atgggatata aagaaaatcc taatccagaa cattataata
     8041 taaatgtaaa gtggtatgag agttggaaaa ttgaaataga gcaaactgct agatatgtta
     8101 aaggtataaa aaataaatat ccagataaaa gtataggtat attggttcca tttaatgaac
     8161 aagtgacaca agttgctaaa gaacttagag agaataatct aacttttgaa gaattaggac
     8221 caaattcttt gaataaaagg aaggttataa acaacatagc atatattatt gattttatgt
     8281 tacattgtga tgatgtagaa aagttaatag ttgtacttaa taaagttttt atagatactg
     8341 ataatgaaga agaaataaag tattttttag aagaattaaa aaactattct actgaagaaa
     8401 ttatttataa ttttgaattt agtaagttag ataatgattt aatagaattt aaagaagaaa
     8461 gtgacatata tttaagtttt aaaagaggaa tagatattat aaaacagata ttagaatatc
     8521 caataataag gttagacctc ttagttttgt ttattgggaa aaaacttaat ttagaaaaag
     8581 aagataaagc tgtactggat tatatatctt tttatataaa gtatctagta tcagaaaata
     8641 taaatatgga tttagaagat gtatataatt tattgtttga tgtaaagaac agggtgttta
     8701 atcatataat agatgtggta tatgagataa atggatatga accagagccc ggtagtataa
     8761 cactatgtaa ctatcataaa tcaaagggga tggaatggga ttgtgtgttt ttactaggtc
     8821 ttgttgaata taatttccct gacaatatta atcaaaagtt tcagagtgat aaatggtatc
     8881 taaaagaaaa atataaaaac cctatggcaa ttattaaatc agaagttgaa gctatattaa
     8941 aaggaagtat ttcaacagat tatgctcata aaacgaagat tgattctata aatgaaaaga
     9001 ttagactttt atatgtaggg attactagag caaaggaaat gttagtactt tcaggaagtg
     9061 cttatagaga tgaaagtgat atagggaata agagaaaaga gcaaaaacca tgtatatatt
     9121 taagcagact taatcaacat attattgaaa aaaggtcaaa ttaatatata ataagttatt
     9181 aaaagattgc ttgaaatata tctaaaaggg gataaaacaa tatgtacagt aagtaactac
     9241 taaaaaataa agttaagaga gaagtgatat gagtgaacga taaattaaaa tatttttctt
     9301 atagtcaaaa ttcaattaac acatataaat catgtccatt gaaatttaaa tataaatata
     9361 ttgatagaat aaattggaaa aatgatgatg ttggtagtag agagtattat gaaactttaa
     9421 aaacaggaag agattttcat ttgatttgtg aaagatattt tagcaatata cctcttggaa
     9481 tctattttaa tgaggatgat aaaaatagta aaaagttttt aaagtggatg gaaaatataa
     9541 aaaaagttgt tcctattgaa aaaggaaaaa cgtatttgcc agaatatgaa gtaaggatga
     9601 cattaaatgg ggatataata caggcaaagt atgatttagt tgtaattgga aataacaata
     9661 tagaaatatg ggattggaaa accgagtcta aaaagattga ctataaacac gtagaaaata
     9721 gaatacaaac tattgtttat atgtttttag caaaagaagt aatacctaaa gttttaaaaa
     9781 tggacataaa tgttaaagat ataaatatga gatattatca acctgaattt gatgacttac
     9841 caattaccat tttatatgat gaagaaaaac atgaagcaaa tagaaataaa attcaaaatt
     9901 atataagtat gataaaaaac actaattatg aagaacatac atacgaagac aatctataca
     9961 atgatataga aaaggtatat aataaagaag aacgtatgtg ctatagaaat aaaaagcatt
    10021 gcaagtattg tgaatttaat aagttatgta atggcaaaga aatagattat agtattttgg
    10081 aggcggaaat ttatgggact taggtttgta ttaggtagaa gtggaagtgg aaaatccacc
    10141 tatatactag atgaaattaa aaaagaagct caaaaaaatg aaacgacatc aattatactt
    10201 ttagttccag aacagtacac atttgaggct gaaaatagag taagtaaact atttttagga
    10261 aaagaaaaag ataagtatct aagagttagg gtacttagct ttaagacact aagtaatata
    10321 gttttttctc aagtaggagg attgacagat gtaaatataa attctagtgg aaaagcaatg
    10381 atggtatata gagctattga agatgtaagt gaagaattaa atgtgttttc aaaatcaaaa
    10441 tcgcagtctg gttttgtaag ttcaattaca gatatgatat cagaaatgaa acaatacaat
    10501 ataagccctg aaatgttaga aaatatatct ggagaattgg ataatgaaac tttaagttta
    10561 aaattaaaag atataagtaa aatatataat tcttttgaag ggaaattgca tgaaaattat
    10621 gtagatgctc aagatatgtt aacttcattg gcaagtaaaa ttgaattaag cagttattta
    10681 gatggagctt gtgtttatat agatgaattt acaggtttta caccaaatca gtacaatgtc
    10741 ataaagagta tattaaataa gtctaaaagt gttaatatct ctttgactgt agatgatatc
    10801 aactatattg ggtacagtaa atctgatatg ttttctagaa cgaaatttac ttattcaaaa
    10861 ttgactcaat tgtgcaacga agaaggcata aaaatattgc cacaagtaaa tttaaacaca
    10921 ggtgttataa aaagatttga aaaagttaag gaattacagc acttagaaag attttataat
    10981 gcttatccat ataaaatata ttcaaatcca actgaaaata taaagataaa agagtttaac
    11041 aatttatatt cagaggtgga agagatagcc agagaaattg tacatttagt tagagataag
    11101 aatgtcagat atagagatat aaccattgct acaagagatt taaatagata tgatttttta
    11161 gtacactcta tatttaatga atataatata ccaaacttta tagataagaa gagagaggca
    11221 aaaagtaatc caatagttat tttaattatt tcagctttag aaatgaagaa tagacgatat
    11281 gggtatgaaa ctatgtttag gtatttaaaa agtggactta taggaattga taatgatgat
    11341 ataaatttac tagaaaatta tgtgttggct aatgggatta aaggtaaaaa atggtttgat
    11401 gaaaaatggg attatagaat aacccaaagt ctttcaggtc aagaaagtga atttgaactg
    11461 gaattaaaag agaaaataaa tgaaataaaa aatagagtat tagagcctat agtaatttta
    11521 caagaaaaat taagaggaaa aaatagagta aaagaaatct gtagatatat atatgaattt
    11581 ttattagata taaatatgcc agaaactata gaaagtttaa tagtaaattt taaggataaa
    11641 ggggaacttg atgttgcaaa tcaatactca caagtttggg acatagtagt ggacatatta
    11701 gaccaaatgg tggaacttat gggtgatgaa ataatttctt tagaaaaatt tataaaatta
    11761 ataaccttag gttttgatga atatgaatta ggactggttc ctcctagtat agaccaggta
    11821 cttgtaagta gtgtggatag aatgaaaaat ccagacacga aatatttata cctagttgga
    11881 actactgatg gtgtgtttcc gctgattaca aaggatagtg gaatattgag tgataatgat
    11941 agggaaagtc ttggaaacaa aggaatcgaa gttgatattg atagtaagac taggagtttt
    12001 gaagaacagt ttcttgtata taaagctctt acttcgacat ctaagaattt aaccataaca
    12061 tatccaatat cagaccatga aggtaaaacg cttagaccat caataattat atcaagactt
    12121 aagaagatat ttccaaatat agaaaataaa agttatttag tggaagaaaa taaaagcact
    12181 gataaagata tacttaaaaa aataacggtt aaatccccta catttaatga acttataaac
    12241 gtaataaaaa attatgacag tgatggttac aatacagaag agattaatag tatttggtta
    12301 gatatatata ggtactattt aaaagatgaa atctatagtt ctataacaaa aaaagttata
    12361 aaaggtctta gttatactaa tcaagttcat aagattgaag agaaaaaaat aaggtcattg
    12421 tataaaagta attctcttag catatctagg cttgaaaaat atgctgaatg tccatttgct
    12481 tactttatac aatatggtct aaaagcaaaa aagcgtaaag agtattcatt tacaccacca
    12541 gatttaggaa cttttattca taatatttta gataggtttt caaaagaact tttacaagat
    12601 aatttaactt ggagagatat tgatgaaaaa tatatagaat taaagatagg tataatagtt
    12661 gatgaaataa ttttaaagat accaggatat atattaaata gttctgagag gtataaatac
    12721 cttgcatata gattaaagaa tatgttgaca actgctataa ctataattag tcagcaaata
    12781 aaacaaggtt catttgaacc aatagattat gaagttaaat ttggcaacaa tggaaaatat
    12841 ccacctataa aaatggtgtt agaaaatgga caagaagtaa gtttaattgg acaaatagat
    12901 agagttgatg aatttgaaga aggagaaaat aaatatataa gaataataga ttataagtca
    12961 ggcaataagt ccataagtct aacagaaata tattatggtc tacaattaca attacttgtg
    13021 tatttagatg caatccttga aagtgcaaag gatgaagata taaatataaa tccagctgca
    13081 atactatatt gtagaataaa taatccaata gcaaaattta atgaagataa agatgatgaa
    13141 gaaatacaag aagcaatatt aaaagaactt agaatgaagg ggcttgtagt aaaagactct
    13201 cacatagtta aagagatgga taaatcatta atagatggag aaaggaaaaa ctctttagta
    13261 ataccagtag gtcttactaa ggatggaaat gtaggtaaaa gtacttcagc aataagctat
    13321 gaggatttca aactgcttag aaaatatgta aggcatgcta taaaggattt atgtgaagaa
    13381 atgttaagtg gagaaatacg tatagctcca tataaacata aagatggaac atcatgtgac
    13441 ttttgtgatt actctgctat atgtcagttt gattcaacta taaaagataa caagtataaa
    13501 aatttaaata ataagagtaa tgaagagata ataaaaatga tgaaaggaga tgttaattag
    13561 tgagttctcc taaatggaca aaagagcaat tagaagttat agaaagtaga gaatgcaatt
    13621 tattggttgc tgctgctgct ggttcaggaa aaacagccgt tctagttgag cgtataattc
    13681 aaatgataac aagtagagaa aatccaattg atatagataa gttattagtt gttacattta
    13741 ctaatgctgc tgcgtcagaa atgagagaga ggatagggga tgctatagga aaagccttag
    13801 atgaaaaccc agaaaataaa catttacaaa accaacttgt tttattaaat aaatctagta
    13861 ttacaacaat acactcattc tgtttggatg ttataaaatc aaactttcat agaataaact
    13921 tagaccctaa tttcagaata ggagaccaaa ctgaatgtgc tattttgaaa caagaagcaa
    13981 tagaggaagt atttgaagat ttatatgaag aaagagatga ggggttttta aatttagttg
    14041 aaagttatgc agaaagagga ggagataaag aagtacaaga tattatcttg ggaatatatt
    14101 catttgcaat ggcttctcct gaacctaaaa aatggttgat tgactcagca gaacgattta
    14161 atattgatga aaattttgat ttttcacagt ctatttgggc tagagctata ttagacactg
    14221 taaaaataga gattaatggt ctttgtctaa atatggaaag ggcattaaaa gaagtggaat
    14281 caatagaaga attagaaact tttgcagaaa agttatccgt tgaatataaa aaaattgctg
    14341 acatttcaca agcatgtaat aaatcttggg atgaggcata taaaaagatg gcaagtatgt
    14401 cctttgaaaa ttatgtaaaa ggagtaaaaa gaatttcaaa agatgctcct tcgtatataa
    14461 aagaatcaaa agaaaaggca aaaactataa gagataagac taagaagtct ttagaaagta
    14521 tagtaagtgc tacattcaat aaagataatg attctattag agaagaaata aaatatcttt
    14581 ataacatagt taaaccaata tctagtgttg tactgagatt tgaagaggaa tatagcaata
    14641 agaaaagaga aaaaggaata atagatttta acgatataga gcattttgca ttaaatatac
    14701 ttacagatgt agatgaaaaa ggtaatattg taccttctga tatagcagtt ggatgtagaa
    14761 acaagtttta tgaaatattt atagatgaat atcaggatag taatctagtg caggaagtac
    14821 tgcttaaagc tgttgcaaat acagaaactc caaatagatt tatggttggt gatgtaaaac
    14881 agagtatata tagatttaga caagcaaagc cagaattatt tttgcaaaaa tataataact
    14941 ataatgataa aaagggaagc tcccatagaa aaataatgct ttacaaaaac tttagaagta
    15001 gagaagaagt tgtagatgct gtaaattata tatttgaaaa tataatgaat gagaatatag
    15061 gagaaattga atatacagaa aaagaaagac taaatttagg tgcaaatttt aatgtggata
    15121 cagacgagaa atctattatt ggtggagcaa cggagataca cctaatacaa aaagataata
    15181 aacttgatga tgatattata aatgataaag acgatagaat taataacaaa gaaaatgaaa
    15241 tagaggaaga agaaaaatta gataatatac aacttgaggc tagaatggtt ggaaacatta
    15301 tcaaagattt aatgaaagtc aatgaagatg gaaaaattca aaaggtctat gataagggaa
    15361 tagatggata tagacctgtg gaatttagag atatagttat tcttttaaga gctacatctg
    15421 catgggctcc agtgtttgca gatgaattga tgaatatgga tataccaacg tatgctgatg
    15481 ttggagtggg gtattttgat acgattgaaa taaaaacaat attgtcatta cttcaaataa
    15541 tagataatcc aatgcaagat atacctctta tatctgtatt aaaatcacct atatttggat
    15601 ttacaccaga ggatttgatt gatattagag ttcagagtaa agataaaatt ttttatgagg
    15661 ttttaaaaag tacagcagag tatgatggat ttacagattc tcaaaatgaa aatgagtctg
    15721 agtttatacc aagtgaagag tgcataaaca aaagtaaaga ttttttaatt aagctaaagg
    15781 aatttaaaga aaagtcaatg tacatgagta cagatgaatt tatatggtac ttgtacacaa
    15841 ggactggata ctatgcctat gttggagcat tgccaggtgg aagtcaaaga caagctaatt
    15901 taaaagtatt gtttgagaga gcaaaacaat ttgaagaaac cagtcttaaa ggaatattca
    15961 actttgtaaa ttttatagag aaattaaaaa aatctagttc tgatatggga agtgcaaaaa
    16021 cacttggtga gaatgcaaat gttgttagaa taatgagtat tcataaaagt aaaggtctag
    16081 aatttccagt agtcatatgc tctgcaatgg gtaaaaattt caatactcaa gattttaaaa
    16141 agagtatttt atatcatcat aatttagggt atggacctca atttgtagac tatgagagaa
    16201 ggatttcttt tccaagtata gccaaagaag cattaaaaag caaaataaat atagaaaatt
    16261 tatcagaaga gatgagagtt ttatatgttg catttacaag agctaaagaa aaattgatta
    16321 taactggttc tactagaaat atacaagata gtataaagag atggtcaaat ggaattgaaa
    16381 gtttagatac aatatcacaa tacgagatat taaaaggtaa aaatttttta gattggataa
    16441 tgccttgtgt attaagacat agggatttat ctaatctact agaagaagta ggattagacg
    16501 cagtttttaa tgttgaacat aattcaaaat ggtatggaaa gttgtggaat aagaatgata
    16561 ttttagtgga aaagaagagt gatgaagaaa aagaaagtat tgaagaaatt cttgaaaaaa
    16621 tagatgtaaa taatcctgac agtgattatt acggtgaaat agaagaaaaa ttaaactata
    16681 tatatccata tgaatttagt acaagaaaac ctgctactat atctgttaca gaaataaaaa
    16741 agatacaaaa taattatgaa gaagagttaa taaatactat atttgaacaa aaagtaatat
    16801 taaaaaagcc tctatttatt caaaatgaag aagaaagaga aaaaataagt ggaacagaaa
    16861 gaggtacaat agtccattta gttatggagg ttttagactt aaaaaatgta agtagtgtaa
    16921 atgatataaa atcacaaata agaggttttg tttcaaaagg tataataaca gaaaaacaag
    16981 ctagtatagt aaatccatat aaaatatata aattttttgc ctctaacata ggaaaaagaa
    17041 tgttgaatgc tgaaattata aatagagaaa aatcaattta tgcacaagta aatatgaaag
    17101 acatatatat ttatgaaaag cttataaaca atgacgataa aaaactttat gataatgaaa
    17161 gtgtcatgtt aagaggtata gttgatgctt attttgaaga agataatcaa atagttttgg
    17221 tggattataa aacagatttt gtaaatgagg aaaatataaa tcaaataata gagaaatata
    17281 aaaaacagtt ggatttatat gcagatatta ttgaaacttt aactggtaag tcagtaaaag
    17341 agaaatgtat atatttattt ggagttgatg aagctgtttg ttattagttt ttatatataa
    17401 attaaaccta aaagatagag gaaggggaaa aaatgagatt tattcataca tcagattggc
    17461 atctagggaa gtccctagaa ggccattcta gaattgagga acaagcaaag ttttgtgagg
    17521 aatttataaa aatagtagaa tcaaatgaaa tagatatggt tataattgct ggagatgtat
    17581 atgatacttc taatccccca gcacaagcag aaaaactatt ttatcagact gtatcaagac
    17641 ttgccaataa tggtcagagg tgtgtattaa taatttcagg aaatcatgat aatccagaga
    17701 gactctctgc aataacacct ctagcacatg aacaggggat acttatatat ggttatccat
    17761 taagtgctac gatagaagct aagtataaag ggtttgaaat aacatatgca actcagggat
    17821 gtacaaaact gaatataaat ggagaaaata ttgtggttgc tacattacct tatccaagtg
    17881 aaaaaagatt aaatgaagtt ttttcaagtg aagatgagtt tgagaaacaa aaaaattatt
    17941 ctgaaaaggt aggagatata tttagaagtt tagaagaaaa ttttagaagt gatactataa
    18001 atatagccgt atcacatata tttgtaatag gtggagagag tactgaatca gaaagaccta
    18061 tacagttagg tggaagtttt ttagttgaaa gaaaagattt gccagaaaag gctcaatata
    18121 ctgcactagg tcatttacat aaacaacaaa aagcttccga acgtttaaat gcatactatt
    18181 ctggttctcc acttcaatat agcaaggatg agagagcgta taccaaaggg gcatacatag
    18241 tggatataaa agctggtgaa aaaccaataa ttgaagatgt atattttaat aattacaaac
    18301 ctatagaagt atttaaatgt aatggaatag aagaagcact agatatatgt gagcaaaatc
    18361 aagatagaga gatttggtca tattttgaaa taaatactga tgagataata tcacagaatg
    18421 aaattaaaaa aatgaaggaa ctattaaaag acataataga aataaaacca ataataacat
    18481 cttgttatga acaagaaagt gtagatataa aagaaaagtc tatggcagag ttgtttagag
    18541 agttctattc ttttagtaaa ggtgtagagc caaaaggaga acttatggat ttatttctag
    18601 atataataag tgaagaaggt gaatcagcag atgagaccaa ttagattaga acttacaggg
    18661 cttaatagct atattgataa acaagttata gactttgaaa aattaattga aagaggtttg
    18721 tttgggatat ttggaactac tggaagtgga aaatctacaa tcttagatgc aataactatt
    18781 gctatgtatg gaaatatatc cagaaataca aaagaatata taaacagtgt gtgcgataag
    18841 gcaattatat catacgagtt tgagatagga agcaaaaaca ctagaagaag atacattgta
    18901 gatagaacca ttgctagaag caaaacagga acaaagacct ctaatgctag attaatagaa
    18961 gtattgaatg acaataccca aaatgtttta gctgataaag ttgtggaagt aaatgaaaaa
    19021 gtagcacagg ttgtaggatt aacagcaaat gattttacta ggtcagtagt tttaccacaa
    19081 gacaaattta atgagtttct aagattatca cgtgcagata gaagagacat gttagagaga
    19141 atttttaatc ttgagaaata tggaagaagt cttggagaaa aagtgaaaaa gagaaaaaat
    19201 atacagttac aaaatttaaa agatttgaaa tcaaaattaa gtcaatatga tggaattaca
    19261 gaggaagtat acaacaatat aaaccaagaa ttaatagagt taaaaaattt ggaaaaagat
    19321 aaggataatg ctttagacct agctcaaaaa tcttatgaag aaagtaaaac agtatatgaa
    19381 gaacaattaa agttagaaaa aaatgaactt agaaaaaatg agcttgattt aaaaaatagt
    19441 gagataaaag aaaagattgc tcttgtagaa aatgatagta acgctaaaaa ggtagaccca
    19501 tacataagtt cagttcaaaa tctagaaaaa aagatagatg aagatagctt cgcagtaagt
    19561 aatttagaaa agaaattagc aatattaaat caggagcttg aagttacaaa aaatagatat
    19621 gaaaaaataa acaaaattaa gaatgaagaa gtccctaaat tgagtgaaga aaaaataaga
    19681 ttgcaacaag caataaaatt agaagaagag ttagttttat tagatagaga gttaaaagag
    19741 ctaaaagaaa gtggaattaa tttaaataag actaaagttg aattagaaaa agtaaagcag
    19801 gtgtcagagt ccagaaaaga tgcagttact aaatctataa aagaagttga aggaaagatt
    19861 gataaagtaa atataagtgc agaattaaaa caaaaaatat ttctagcata tgaatacgag
    19921 aaagattaca acaacgtatt ggaagaaaaa aatcaaaagc taaataaact agaagaaata
    19981 ctcaaagaga cagaaaatat aaatttaaaa gttagatata ttgataaaga taagaatgat
    20041 gtaaatcgta atttagaaaa tttatcactt cacttggatg ttcttcttaa aaaatgtcct
    20101 ggtaagagtg ctgatttact tttaaaatct gaatatgtta ctgaattaag aaataaagca
    20161 aataatacta aagaaaatga aattaagaaa agcagtatac aagatgaatt aaagataata
    20221 cttgaaagta agtttaatac tgaaagggaa gtaaatttac taaatgaaaa acttgaaaat
    20281 aatagaaaga acagagatga cttagaaaaa gaattagaag aactaaagta tttaaattta
    20341 gcctctgaac ttagacgtga attaaaagaa aatatgcctt gtccagtttg tgggtcaaaa
    20401 catcatgaaa atcatgatat aactaagtat gatgaaaata tatcttttgt caaagaaaaa
    20461 ctggagaaat tagaaaaaga aaaaataagt ataagaaaca atatagagga attaaatgct
    20521 aaagtaagtg ggtatttatc tatagaaaaa atgaagacta aagaacttga agatgtaaaa
    20581 ggaaaactag gggggattcc atctagtcaa ttattaaaaa aacttgatga agaacaaaga
    20641 aaacttgcat tgttaaaatc taatattcag gagtgggaga aagaaaaaga gagtactgaa
    20701 aataaagtaa cattagctaa agaagaaaaa aataaaattg aaaaagaaga attaaaaata
    20761 agagagagtc tgaataatta taagaaatta acaagagatt taaacataga aatagaaggt
    20821 ttagaaaata aatgtaaaaa acttaaacag gaatacctag gattaaagac gattactaaa
    20881 gtctctaatc tattgtcaaa agtaaaagaa ataagagaga atgaaaaaga attagaatta
    20941 ctaagtactg ggcattcaaa tttattaaaa aatagagact cactagatat taaaataaga
    21001 gaaaatgaga gtaaactaca tgaaatagag atagaattaa taaaggctag agaattatat
    21061 gtagaaaaga aattgagtag agataataag tataaagaag ttattagcat aacgaaaggt
    21121 gatttagcta aaaatctttt acataatgtt gaagaaaata tatgtaagat attagaacaa
    21181 gaagaatcaa gtaagaaaaa attggaagag caaagattag aatatgaaaa aaacattgct
    21241 gaaaagcaca atatagatgg tagacttaaa actgctaagg aacaatataa agaccaaaaa
    21301 tcaacactaa ataaactatt agcagaaaat aaatttgaaa gtatatatgc agtaaaaaga
    21361 gctttattag atactgatgc agtaaaaaaa ttgattgaag aaatactaga gcatgaagaa
    21421 gaacaaaagt tattatcatt taaaataaaa tcatcaaaag aaaaattaaa tggaagaagc
    21481 ataaagaagg agtactttga acagttaaaa gatgaaattt ataatttgaa agtagaaata
    21541 ggaaatatat ccaaagatat tggagctaat caaaatcagt taataacatt aaaagactct
    21601 ttagataaga taaatgattt taataaacaa cttaaggtag ttgaacacaa tgtggactta
    21661 ttggaagaat tagataaatg tgtacaagga aataaatttg tagaatatgt ttctacaaac
    21721 caattaaaat atatagcgtt ggaagcatca aaacgattgg atggaataac aaaaggaaga
    21781 tatgcacttg aaattgactc aacactaaac tttgtaatga gagataactt taatggagga
    21841 gaaagaagaa gcattgatac tctatctggt ggagagacat tcttgacatc tctatcatta
    21901 gcacttgctc tttcatctca aatacaatta aagggaagtg caccattaga atttttcttt
    21961 ttagatgaag gttttggttc attagatagt gaacttttag aaattgttat agaatcattg
    22021 gaaagattgc atagtaataa tctaagtgtg ggtataataa gtcatgtcga ggaacttaaa
    22081 aatagagttc ctgttaagct attagtttca tctagtgagg ctggaatagg ttcaaaagta
    22141 aaaattgaat atagttagaa aatatatgtt aattgaatat attatctaat atttccaaat
    22201 aatatgtaat tgtgatataa taaaaactaa tattaaagtt atttataatt aatattaata
    22261 taaaaacatg taagtaggag tgtggtaagt gttggaatcg cccaataatt tgattcagat
    22321 tattttttta atagtattac ttatagggtc tgcatttttc tcagcatctg aaacggcttt
    22381 aatgtcgttg agtaaaatca gaatcagata tatgcaagat gaaggagtta aaggagctaa
    22441 gttagtaagt tcattaatag aaaatccaaa taagctttta agttctatat tggttggaaa
    22501 taacgtcgta aatatagcag caacatccat atcaacatct ttgtttatag gattaatggg
    22561 agaaaaagga gtggcacttg ctacagctgt tatgacagta ttagtattaa tatttggtga
    22621 gattactcca aaaacaatag cagcaaataa ttcagaaaag gtttctctat tagtatctaa
    22681 acctataaaa gcaattatat ttatattaag accaatagta tggatattta atataattac
    22741 aaatattata tttaaattat ttggtataac aaataaagga gctaaatcat tcataactga
    22801 agaagagcta aaaactatgg taaatgtaag tcatgaagaa ggcgtccttg aaatggaaga
    22861 gagagaaatc ataaataatg tttttgaatt tggagatatg caagctaaaa atgcaatggt
    22921 acaaagaata gatatggtag ctatagatat ggaagatagt tatgatgaga taatacaggt
    22981 atttaaaact gaaaaattaa gcagaatgcc tgtgtatgaa gaaactatag atgatattgt
    23041 aggaatactt aatataaaag atattatatt tttatctgat gaagaaatag agtcatttga
    23101 tattaaaaat tatatgagag aacctttttt cacgtatgag ttcaaaaaga taacacaact
    23161 tcttgaagag atgaaacttg aaaaaagtca gatggctata gttgtagatg agtatggtgg
    23221 aacatctgga cttttaacta tagaagattt agtagaagtc attgttggag atatagagga
    23281 tgaatatgat gaagaagagg atgaaataca agttataaaa gaagatgagt atattgtaga
    23341 tggaagtact aaaattggtg atgttaatga acttattggt gtaaacttgg aatctgagga
    23401 gtttgattcc ataggaggtt ttataattgg acacctaagc agattgccag aagaaaatga
    23461 agtaattgag gttgataata taagattttg tatagaaagt atagaaaaaa atagaattaa
    23521 aaaaataaga atatatacat aatgtaataa t
//
LOCUS       DAYAPA010000057        23235 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.057, whole genome shotgun sequence.
ACCESSION   DAYAPA010000057 DAYAPA010000000
VERSION     DAYAPA010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23235
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.057"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            205..1437
                     /locus_tag="ACGI59_002051"
     CDS             205..1437
                     /locus_tag="ACGI59_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HFG9739837.1"
                     /translation="MQEREELVLNPVPKEERVGWLAPLFNMLGSNIAISELMVGGTLI
                     LGMTLSNMIITSIIGNLILVAIIMIQGYIGYKEGLNTYVLAKGAFGEIGGKYLISLLL
                     GITSFGWFGVQAGVAGLSVQKIFPSVNLTLVTVILGLFMVVFALYGFKAMAKFNYLVI
                     PPLMILMVWGVFKAFSTYGVEAIYNYTPQTTMSMVEGLNIVVGLVIVGAIISPDQLRY
                     TRRVKDIWIISFLGLGIISLFQQVAAGVMSMGAPTWDITEVLANLGFGWVAFVILILA
                     SWSTNVSNAYSGGLALKTIFPNVKRNVLTLVAGLIGTIIAATGIIFKFQSFLSFLGIA
                     VPAIAGIMWCEYYFIQGRTYKHREGINWIAVISWLIGFAASYYSSKINFLIPPINGIV
                     VSMVIYYILMNCFGIKDK"
     gene            1451..2257
                     /locus_tag="ACGI59_002052"
     CDS             1451..2257
                     /locus_tag="ACGI59_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HFG9739838.1"
                     /translation="MGRKVKIGIIQQHSVLGNVKKNIEKAVEMIDDLGKQGADIICLP
                     ELFATGYNLESLGGVKTLELIREHNKYIEESMSEAAKRNNVYLISPYGTLEKGSTHVY
                     NSAVIFDRKGKIMGEYCKNHLWSLEAVYFKGGEKVEVYDADFGRFGVMICYDAGFPEV
                     SRELTLKGSEIIFIPSAWRIQDEDMWDLNVSQRALENTVYTVGVNLVSNDSNLILFGK
                     SKICNPRGTVITQLDTYREKYVLSEIDLDEIKKYREDIPYLKDRKSSYNI"
     gene            complement(2369..3238)
                     /locus_tag="ACGI59_002053"
     CDS             complement(2369..3238)
                     /locus_tag="ACGI59_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFG9739839.1"
                     /translation="MDLLHLKYFQTVARMEHITKASHKLNIAQPALSKTISSLEKELG
                     VQLFDRKGRYIVLNEYGRLFLKRVDSILDLVESSKKELQDTSLENSGEVKILSPAAAN
                     VLPSLLSNFRKLYPNITFNVSHTLPSSYKKSDFDLYISSSFTKLNSENSITLTCEEIL
                     LGVSINHPLSLKDEVYLSEVSDENFIVITKGENYREVIDILCESANFKPKIAFESDSP
                     YTIYALIKSLQGVGFICGKSWGLSQDPEIKLLHIKDIEFKRYLNLSWFSENYESKAVL
                     LFKNFLINYFKNI"
     gene            3610..5037
                     /locus_tag="ACGI59_002054"
     CDS             3610..5037
                     /locus_tag="ACGI59_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp14"
                     /protein_id="HFG9739840.1"
                     /translation="MKLTKSISSMLCLSLVLSLSPVAYANEISRTTVENTLVGQDRYQ
                     TAVEVSKKGWSSANEAVVVNSESIVDALAVTPFAKLKNAPILLTEKNNLNSQTEKELK
                     RLGVKKVYIIGLSSSVSNDVQKQLESNNLTTDRVGGSDRYQTALSIAKKIEGLKDISE
                     IAVVNGYTGLADAVSIASVAATNGMVILPVSDASGISSFKDFIESKNINKSYIIGSTN
                     AVSDKIKQSLPNSERIGGADRNETNGKVIEKFYTSNKLNNVFVAKDGMKNQNQLIDAL
                     AVGVLASKENSPVLIVGEQLSATQKSVFSNKNATSITQVGSGGNENAFKELKDLQANS
                     NNKRVMYVTNTDKVNIRNDATIEASVIGYLNNGDEVEVLDVLKTGWVEIKYNEGIGYV
                     SGSYLTNNKPDNSNENIKIKYVKEKDGLNVRKGPSTEDEKIGHLSYGSKVETIEMFAT
                     GWVKIKYNGGYGYVSNDYLSDTPVI"
     gene            complement(5114..5383)
                     /locus_tag="ACGI59_002055"
     CDS             complement(5114..5383)
                     /locus_tag="ACGI59_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9739841.1"
                     /translation="MKFEYYYLIQDIAGILLAFIGLRMSIIGFRILSMKGLSINTLLI
                     VIKYCLFTIAGLNLLISKFGIRHWIWSVCMLIISIIINPRIKVSK"
     gene            complement(5386..6828)
                     /gene="nhaC"
                     /locus_tag="ACGI59_002056"
     CDS             complement(5386..6828)
                     /gene="nhaC"
                     /locus_tag="ACGI59_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFG9739842.1"
                     /translation="MKMNTERKIPSFRYSLLVMLAIIALTSVGIVVFNASITTMFLLS
                     WLIVVPAAMKLGYTNDEIEAFGFEVGKDAFQSNLIILSVGVLIAAWIAAGTIPTVVYS
                     GLTIITPKYFLLTTLIVCSLTSVATGTSWGTLGTSGIAMMSIGTSMGIPLGLTAGAVI
                     SGAFFGDKMSPLSDSTNLAAAVCKTDVITHMKHMFYTTGPAYVICIVLYTVIGFKYSN
                     NTIDYVQINQIKDVLNSNFHIGLVAMIPIIFLLLLLLLQKPPIISILSSAIMGLIIAV
                     FQEGEKIGDVLNYMLSGFTIDTGFVYADKLLNRGGIMSMAETVLLVFVVFVIAGILQK
                     TGFLEVLLQPLITKIGKSRTKLVGSTFIVSYFANAFSSSMMFTSVFVGTLMSPIYKEF
                     KLKPQNLSRIIEDTATLGGPLIPWNSNAVFCAQTLGVSPLEFIPYCFLSWITPIISFI
                     YGVTGFTMLTYTDDEIEELEIFDTAQRRVD"
     gene            complement(6853..8019)
                     /locus_tag="ACGI59_002057"
     CDS             complement(6853..8019)
                     /locus_tag="ACGI59_002057"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFG9739843.1"
                     /translation="MNYNFNEIVDRSNNFSSKWSEMEKKYGTNDLLPMWVADMDFKAA
                     PCIIDSLKNRLEQEIYGYTTRPDSYNESIVNWLDRRHNWKIKSEWLIYSPGVIPAISL
                     LINELTKANDKIMIQEPVYSPFNSVVKNNNRELIISPLQKLENGNYIMDYEDIENKIK
                     DVKLFILCNPHNPVGRVWTKDELKKLGDICLKHNVKIISDEIHSDIILKKHKHIPMAS
                     ISKEFEKNTITCMAPTKTFNIAGLQSSYVILPDEKDYKLLDDAFTRIDIKRNNCFSLV
                     ATEASYNNGESWLESFLEYLESNIDFAIKYINENMPKLKVRKPEGTYLLWVDFSALGL
                     SDEELESILVQKGKVALNQGNSFGIGGSGYQRINLACPRSMLEEALIRIKNAIN"
     gene            complement(8244..10364)
                     /locus_tag="ACGI59_002058"
     CDS             complement(8244..10364)
                     /locus_tag="ACGI59_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFG9739844.1"
                     /translation="MKQMELAIVSLKKDAGEIYENQIRQFLGDNLKINLYSFEEGNLK
                     FFKEKLILLSAYLKYDEIVKLSHYDAQIIVPKLTFEKNSIDMISKLEKDKIIYVYNLS
                     KDMAIETISLIHRLGIDNINILPCYPEIEFTPTDAVILTPGEKILPKFKNCEVIDLKY
                     RIIDLSCIVEIATKTKLKHLIKDDLIKKYVEKIIPTSYSTGELLLDANKFERQFDLLL
                     SIIDDGIICTNNDGIIQFYNHMARKILSINANEMIDSFVGDCIKDINFQSILTNKTPF
                     FEKLIKINHIDINLEIKHIQLNVFDGFILKMTKFSQLEKKQAKLRAQLVNSGNISKYT
                     FDDILGSSIQTINTKKIANKMAQSNSSILIIGESGTGKELFAQSIHSASRRKDGPFVA
                     VNCSTFQENLLQSELFGYDEGAFTGAKKGGKIGLFELANNGTIFLDEIGEMDLNSQSK
                     LLRVIQEKQVRRIGSNNVIDIDVRIIAATNRNLKELVSKNMFRRDLYFRLNVLPLKIH
                     PLRERPADIFEIFGSLKYDIPCNFILSEEVKEIFKMYRWEGNVRELRNLGEYFCYLEK
                     DIIEICDLPEYILDTIDSNYSRTVCNKVSDNIKKYQFNIGKDKNIMKYDYNFKRSLDE
                     YIFILDNLKKAYDLKERIGRKSLCKIALEENRFLTEQQIRNMLLELQNFGLVDILVGR
                     GGSIITSKGVEFLKNINRSNKLNS"
     gene            10597..10998
                     /locus_tag="ACGI59_002059"
     CDS             10597..10998
                     /locus_tag="ACGI59_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9739845.1"
                     /translation="MKKCLYCNRDIKSPNNKLAIKYDDNMNIYPCRAECKEKIEEYIA
                     KKPTYKFINILEVLLGVGGIFCFLSKWTIAAQVVLGIDALVIFLFPLAGFKMNGKLSM
                     EQTKNQSRSIAISILAFIVIITVLYFKQVGK"
     gene            11041..11334
                     /locus_tag="ACGI59_002060"
     CDS             11041..11334
                     /locus_tag="ACGI59_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HFG9739846.1"
                     /translation="MEERKLAPNNEREALIKRLKRIEGQVKGIQKMVEQERYCVDILV
                     QISAIRSAINRVGTIILENHIKGCVAESIKHDDADKTEEMIKELMDTINKFTK"
     gene            11349..11789
                     /locus_tag="ACGI59_002061"
     CDS             11349..11789
                     /locus_tag="ACGI59_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HFG9739847.1"
                     /translation="MNQQGFIMEIVDDRTAKLKMQRHSACAACGKCATTNSAESKEIL
                     VEVDNTIGAKVGDHVEVSMDNMNVLKAAVMAYIIPLIALLVGTIGTYYILGAIGMTTG
                     VEAISGLVGICLTIICYLYLKKNDKKFRDSREFIPVITRILIDL"
     gene            complement(11881..12354)
                     /locus_tag="ACGI59_002062"
     CDS             complement(11881..12354)
                     /locus_tag="ACGI59_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFG9739848.1"
                     /translation="MTDMSMILHFLFSGVATAGFAVFFNAPLYLLLPAGVTGGIGWIV
                     YVYLFNFTTNAVFAGFIAAALVSACSETLARKLKQPAIVFVIPGILPLIPGIGLYNTM
                     LSLIQKNYSLAMSKGTDALFLSAAIALGVLVVTSFVRTLNLLKIRKNFIPYRKSK"
     gene            complement(12354..13142)
                     /locus_tag="ACGI59_002063"
     CDS             complement(12354..13142)
                     /locus_tag="ACGI59_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFG9739849.1"
                     /translation="MNQNEELEYKKNILKLALFVGELMIKNGAETSRVEDSVLRICRS
                     RGFYHVNIFTTPTVIIISDEKFDGFSFMKTIQSRGINLNKISLLNSFSREFVSDKEYD
                     IDNAIARLYEIQDVKPYPLWLFYSCTGIASACFACLLGGNYVLNFILTFIIAIFAAIT
                     YDKTMKISAISAFSCLVSSFLIALSGVLLVEIGILDDPKMLIVGAIMPLLPGVAFIKA
                     IRDLISGNLISGISRAFDAAMIIIAIASGVGFVLDTWYRIGGPF"
     gene            13470..14714
                     /gene="glyA"
                     /locus_tag="ACGI59_002064"
     CDS             13470..14714
                     /gene="glyA"
                     /locus_tag="ACGI59_002064"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFG9739850.1"
                     /translation="MFENLKKADPEIYESMKRELKRQQRNIELIASENIVSVPVMETM
                     GSHLTNKYAEGYSGKRYYGGCEYIDEVETLAIDRIKKIFGAEHANVQPHSGANANIGV
                     YFAMLKPGDVVMGMNLSQGGHLTHGAPVNISGQYYKFYEYGIDKDSGLIDFDEVRKIA
                     HEVKPKMIVAGASAYPREIDFKKFREIADEVGALLMVDMAHIAGLVAAGLHQNPCEVA
                     DFVTTTTHKTLRGPRGGVILCKEKYAKDIDKAIFPGIQGGPLEHIIASKAVCFKEALS
                     DEFKEYQVQVAKNAKALAEELIKRDFKLISGGTDNHLILLDLTNKNITGKAAEKRLDD
                     AYITANKNTIPFDPNGALITSGIRLGTPAVTTRGMKEEDMAIIAEAIDLCLTYDEESK
                     ARTLVVGLTEKYPLYEEYNIMD"
     gene            15037..16134
                     /locus_tag="ACGI59_002065"
     CDS             15037..16134
                     /locus_tag="ACGI59_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFG9739851.1"
                     /translation="MKVLILTGKFGMGHYSASNSLSEDIKAKFDNSEIIIKDIFEYIM
                     PNYSDKMYKTFSILVNRGSSLYNLFYKCAENGKKDIKFTFSDYFLNKLDTLLHEVQPT
                     VVISTFPFCSQLVSRYKEKYNSNLPLITCITDISSHSEWISKNTDCYLVASKSTKEEL
                     VFKGIDESKIKVNGIPVKKEFKRIEHVNHSTKKNILIMGGGLGLLPKSEQFYKELNSL
                     EGVKTTVITGNNKKMYYKLYGRYENIEVVGYTNEVYKYMKDSDLIISKPGGITLFETI
                     YSELPILAFNPFLQQEIDNASFILNNEIGRILGKNKKYYVDEIKDLIYDDATLKEMSS
                     NMKELKKQFDNNTLENILFSLDEQGACRECM"
     gene            16125..16823
                     /locus_tag="ACGI59_002066"
     CDS             16125..16823
                     /locus_tag="ACGI59_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFG9739852.1"
                     /translation="MYVVGLIIVVALIFLVHSIIPTYYNKLLNKEVLKNMAGENEIAL
                     TFDDGPDKRYTEKLLDVLKENDIQAMFFVVAKNAEKEPEIIKRMLRENHIVGLHSLEH
                     RNAWLYSYSYVKKDFIESTNIMKNLGVDVNYYRPPWGHTNIFSNSFVKKYNLKMTLWD
                     VMAEDWEKDSTVDIIINKLMSRTKENSIICLHDAGENSGGAVGAPERTIEALKIAIPK
                     LKASGLKFVTPERM"
     gene            16826..17890
                     /locus_tag="ACGI59_002067"
     CDS             16826..17890
                     /locus_tag="ACGI59_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HFG9739853.1"
                     /translation="MISKTEKKNKCLGSVAFLVLLMGITGYFVFRGQSVESLIKSLKG
                     ASPMFILIGFAMMFIYVACEGINIYLGMKALNQKTTLLKCMGYAFIGFYFSSITPSAS
                     GGQPAQVYYMKKDDINISYSSLILLVIVVIHQVVILAYSGIMFIMEREFILNNVSGMN
                     ILLIYGVITNVALVIGVIAIIFSKKLVNNFIISITNLLGKLRIIKDVESSRKVIISQI
                     EEYVKGAQYIKQNPKLVVQILVITIVQITAMFLVPFFVYKAFHLSTYTVFEILAIQSL
                     LNIAVSSLPLPGAVGASENSFMTLFKIFFPGHLLVPAMLLSRGISFYAFVAISGLICI
                     VVHVKSSEKVKAVKKVAYVR"
     gene            18073..18777
                     /locus_tag="ACGI59_002068"
     CDS             18073..18777
                     /locus_tag="ACGI59_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFG9739854.1"
                     /translation="MNKEIPKKILVADDNPEIREIVEILLTGEGYEVIMATNGEEAVN
                     LVDSTIDLIILDVVMPVKTGFVACNEIREKTTAPILFLTAKTQDSDKVIGFSAGGDDY
                     LSKPFSYSELISRVKSLLRRYYIYQGKEKEREPNILKTKELCVNLDTQDVTLNGEPVF
                     LTTIEYSILILMLKNRKKVFSSENLYETIWDEQYFYTANNTIMVHIRNLRKKLEKDQK
                     NPQYIKTAWGKGYYID"
     gene            18770..20092
                     /locus_tag="ACGI59_002069"
     CDS             18770..20092
                     /locus_tag="ACGI59_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HFG9739855.1"
                     /translation="MINKIKPSKLGVILLIYVVISAAVAFILNIPFYSAVESIVKDVS
                     EGKVYFKREVNQLMDNFQTYVNNNGIRADDGDDIAKWNSDNWFVSMKIYKGNKVFYDT
                     LYYPNEVPDKSEENEFYSSPMARNYEIQFSDIKANIVVVALFRFRFQNFIEAISITFM
                     ALTFIITFLLLFSRKIKYLRKIEMGIKIVESGSLKYRIPVKGNDELSSLASSINEMSH
                     TLENQLIKEREMKNKNKDIVTSISHDVRTPLTSVICYLDLIKDHKYNSPEQLEQYIKN
                     VRSKAYQIKDLTDDLFTHFVNSGEEVSKGYEKINANEFIFQLLLDSSYTLEEKGFHIT
                     IENELYNQYHIYVDVTQFRRIFDNLCSNIIKYARKDKPIVFLIKVEDSKLLIIQKNRV
                     SNFSKNVESFGIGIKNCEKIVAYHNGSINVNSDDKEFAIEISIPAKES"
     gene            20279..21652
                     /locus_tag="ACGI59_002070"
     CDS             20279..21652
                     /locus_tag="ACGI59_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9739856.1"
                     /translation="MVLTGAHYIYILFMVIILITMIMKKDTIVPCILGVFFMGLFFEK
                     NIFGAITAVFNSFIISLNELGPIILIIAIMVALSKALEANNAIQYMVRPFSRVIKNSN
                     TAFFVTGFVMLVLSWFFWPTPAVALVGAVFLPVAMRAGLPAIGVAVALNLFGHGLALS
                     TDFVIQGAPSITAGAAGVAVSYVINDGMILFWVMGIVTISMAFYTLKRDINKGMFREE
                     LKGFESEEVEEFNGKSKIATILVALGFLADIIAMYIFDLKGGDASALLGGTAVFLIII
                     INVINFGKDSLENVCENIIDGFVFGIKIFGAIIPIAAFFYMGEVAPLTGVFGKVLAPG
                     SQGLLSDIGIALSQTIPLNKFAVSGIETVVGAITGLDGSGFSGISLVGSLASVFGTAI
                     NASVGALAALGQISGIWVGGGCLVPWGLISAAAICGVSPIELAKRNFVPVITGLVVTT
                     IVAMFII"
     gene            complement(22024..22962)
                     /gene="pdaA"
                     /locus_tag="ACGI59_002071"
     CDS             complement(22024..22962)
                     /gene="pdaA"
                     /locus_tag="ACGI59_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="delta-lactam-biosynthetic de-N-acetylase"
                     /protein_id="HFG9739857.1"
                     /translation="MFRKVLHYLTLLSISIFFIVGCSNSQNNQNENQNKETQLQEDKE
                     KIDSGKDTSNVIVSDGTDKPSKATTNNDNNKLDVSSLDNTTLDWFYIPNNKHKTPEVN
                     TDIEFKFSDYDALYNGPTKDGQKTLYLTFDEGYENGYTTKILDTLKQNQVKAVFFVTA
                     PYIKENKDLVKRMVSEGHIVGNHSKTHPSMPTKTSNLKNFNDELYDVEKLYKDVTGKD
                     MVKFFRPPMGKYSEKSLAMTKNLGYKTVFWSFAYRDWDTDKQPSHEEATQKIMDNLHD
                     GSILLLHAVSKTSTEILNDFISNARKLGYEFELLEY"
BASE COUNT         8561 a         2890 c         3674 g         8110 t
ORIGIN      
        1 atgacttaat agattaaatt aaaataataa atttggacta tttatctatt tataacttaa
       61 attatatcaa attgataaca aactagatat tatacttttt tgtagaatag aattacaatt
      121 atataaagaa aaattctatt aatagtgaaa aaaaagtaga aaaaagtaga aattattctt
      181 ttataaagaa agggagggat aatattgcaa gagagagaag aattagtttt aaatccagtt
      241 ccaaaagagg aaagagttgg atggttagct ccattgttta atatgcttgg ttctaacata
      301 gctatatctg aactaatggt tgggggaact ttaattttag gaatgactct ttctaatatg
      361 attattacat caattatagg gaacttaata ttagttgcaa ttattatgat acaaggttat
      421 ataggttaca aagaagggtt aaatacgtat gtattagcta agggggcatt tggagagata
      481 ggaggaaagt atcttatatc tctattactt ggtataacta gttttggatg gtttggtgta
      541 caagcagggg ttgcaggact atcagtacaa aagatattcc caagtgtaaa cctaacatta
      601 gttacagtta tacttggatt atttatggtt gtttttgcac tgtatggttt taaagctatg
      661 gctaaattta attacttagt tataccacca cttatgatat taatggtgtg gggagttttt
      721 aaagcttttt ctacttatgg agttgaagct atttataatt atactcctca aacaactatg
      781 agtatggttg aaggtcttaa tattgtagta ggtcttgtta tagttggtgc aattatatca
      841 cctgaccagt taagatatac gagacgagta aaagacattt ggattatcag ttttttaggt
      901 ctagggatta tttcattatt ccaacaagtt gcagctggtg taatgtctat gggagcacct
      961 acttgggata taacagaagt attggctaat ttaggttttg gatgggtagc gtttgttatt
     1021 ttaatacttg catcttggtc tacaaacgta tcaaatgctt attctggtgg attagcatta
     1081 aaaactattt ttccaaatgt aaaaagaaat gttttgactt tagttgcagg tctaataggt
     1141 acaataatag cagctactgg tataatattt aaatttcagt catttttatc tttcttggga
     1201 atcgcagttc ctgcaatagc aggaattatg tggtgtgagt attattttat acaaggaaga
     1261 acttataagc atagagaagg aataaattgg attgcagtaa taagttggtt aataggtttt
     1321 gcagcgtcgt attatagttc aaaaataaat ttcttaatac ctccgataaa tggaattgta
     1381 gtgagtatgg ttatttatta tatattaatg aattgttttg gaatcaaaga taaataaagg
     1441 gggcaattac atgggtcgta aggtgaaaat aggtataatt caacaacaca gtgtacttgg
     1501 aaatgttaaa aaaaatatag aaaaagcagt agagatgata gatgacttag gaaaacaagg
     1561 ggcagatata atttgtttac cagaactatt tgctacagga tataatttag agtctttagg
     1621 tggagttaaa acattagaat taataagaga acataataag tacatagagg aatcaatgtc
     1681 agaagctgct aaaagaaata atgtatacct gatatctcca tatggaacct tagaaaaagg
     1741 gtctacacat gtttataatt cagcagttat atttgataga aaaggtaaga ttatgggaga
     1801 atactgtaaa aatcatctgt ggtcattaga agcagtatac tttaaaggag gggaaaaagt
     1861 agaggtatat gatgctgatt ttggtagatt tggtgtaatg atatgttatg atgcaggttt
     1921 tccagaagta tccagagaat tgacattaaa aggttctgaa ataatattta taccatctgc
     1981 atggagaata caagatgaag atatgtggga tttaaatgta tctcaaagag ctttagagaa
     2041 cactgtatat acagtaggtg taaatttagt aagtaatgac tctaatttaa tactatttgg
     2101 aaagagtaaa atatgcaatc ctagaggtac tgtaataact caattagata catacaggga
     2161 aaaatatgta ttatcagaga ttgatttaga tgaaataaaa aagtataggg aagatatccc
     2221 ctatttgaaa gacagaaaaa gtagctataa catataattc tatactataa tacaagttca
     2281 atataaaatg ataaaaagag gatgtatcaa tattaagatt aaatttatta tactttgata
     2341 tatcctcttt aatattatca actttgattt aaatattttt aaaatagttg ataagaaagt
     2401 ttttgaacaa caacacagct ttagattcat agttttcgct aaaccatgaa agatttaaat
     2461 atcttttaaa ttcaatatct ttgatatgta acaacttaat ctcagggtct tgagataatc
     2521 cccatgattt tccacatata aatccaactc cttgaagtga ttttataaga gcataaattg
     2581 tatatggtga atcagactca aatgctattt tgggtttgaa attagctgat tcgcacagga
     2641 tatcaattac ctctctataa ttctcaccct tcgttataac tataaagttt tcatctgaaa
     2701 cttcgcttag atatacttca tcctttaatg ataaaggatg gtttattgaa actcctaata
     2761 gtatttcttc gcatgttaaa gttatgctat tttctgagtt tagctttgta aatgaagaag
     2821 atatgtacaa atcaaaatca ctttttttat aagatgatgg caaagtatgt gacacattaa
     2881 aagtaatatt gggatatagt tttctgaaat tactcaaaag tgatggtaac acattagccg
     2941 ctgcaggaga taatatcttt acttctcctg aattttccaa agaagtgtct tgtaactcct
     3001 tttttgaact ttctactaaa tctaaaatgc tatctactct ttttagaaac aatcttccat
     3061 actcatttaa aacaatatat cttccttttc tgtcaaataa ctgaactcca agttcttttt
     3121 ctaaggaaga tatagttttg cttaaagctg gctgtgctat atttaacttg tgtgatgctt
     3181 ttgttatgtg ctccattcta gcaacagttt gaaaatactt aagatgcaat aaatccatag
     3241 tataaaatac acctctttta atatgacttg agttatatca attttataat caactccaca
     3301 ttatacatta taatataaag tttatataat aaactacaat atattaaaaa gttatttaaa
     3361 ttgttattta tattttgtac ataatctaat taaaaaaagt aactttaaat ggttaactaa
     3421 ggaaatattg ttacaaactt tagttatata aaaaattcgt ttgatgagca aagtattaat
     3481 gtcaagtaag aataaaagct tattagataa gaaaaataat attttataat aatgatgtta
     3541 cttaaattat tatgttatac tattttaaat aggaaacatt tagatactat aattttaggg
     3601 ggagtcaaga tgaaattaac taaatcaata tcatcaatgc tgtgcttatc tctagtttta
     3661 agcttatcac cagtagcata tgcaaatgaa ataagtagaa caacagttga gaatacttta
     3721 gtaggtcagg atagatatca aactgctgtt gaagtaagta aaaaaggatg gagtagtgca
     3781 aatgaggctg tagttgtaaa tagtgaatct attgtagatg cattagctgt aacaccattt
     3841 gctaaattaa aaaatgctcc tatattattg acagaaaaaa ataatctaaa ttcacaaact
     3901 gagaaagaat taaaaagatt aggagttaaa aaagtttata taattggtct atctagttct
     3961 gtatcaaatg atgtgcaaaa acagttagaa tctaataatt taactactga tagagttggt
     4021 ggtagtgata gataccaaac agctttgagt atagctaaaa aaatagaagg attaaaagat
     4081 atatcagaaa tagctgtagt aaatgggtac acaggtcttg cagatgcagt tagtatagct
     4141 tctgtagctg ctactaatgg aatggttata cttccagtat ctgatgcatc tggtatatct
     4201 agctttaaag attttataga atctaagaat ataaataaat catacataat aggctctact
     4261 aatgctgtat cagataaaat aaaacaaagt ttacctaatt cagaaagaat aggtggagca
     4321 gatagaaatg aaactaatgg taaagtaata gaaaagtttt atacatctaa taaattaaac
     4381 aatgtatttg tggctaagga tggaatgaaa aatcaaaatc aacttataga cgctttagca
     4441 gtaggagtac tagcatcaaa agaaaattct cctgtactaa tcgtaggaga acaattaagt
     4501 gcaacacaaa agagtgtatt ttcgaataaa aatgctacct ctataactca agttggtagt
     4561 ggaggaaatg aaaatgcatt taaagaatta aaagatttac aggcaaatag taacaataag
     4621 agagtaatgt atgtaactaa tacagataag gtaaatataa gaaatgatgc tacaatagaa
     4681 gcttctgtta taggatatct aaataatggt gatgaagtag aagttttaga tgtgttaaaa
     4741 actggatggg tagaaataaa atacaatgaa ggtattggat atgttagtgg gtcttatttg
     4801 acaaataata agcctgataa tagtaatgaa aatataaaaa taaagtatgt aaaagaaaaa
     4861 gatggtctaa atgttaggaa aggaccaagt acagaagatg aaaaaatagg tcacctttca
     4921 tatggaagca aagtagaaac aatagaaatg tttgctactg gatgggtaaa aataaagtat
     4981 aatggtggat atggttatgt aagtaatgat tacttatctg atacaccagt tatttaatta
     5041 taagtaacaa taaaaaaact gctttaattt gtaaagcagt ttttttataa aaatttaagt
     5101 ctaataacta tttctatttt gataccttaa tcctgggatt tattattatg gatattatca
     5161 acatacaaac actccaaatc cagtgtctaa ttccaaactt agatattaat aaattcagtc
     5221 ctgctatggt aaataagcag tatttaatta caattaaaag tgtatttata gaaagcccct
     5281 tcatagaaag tatcctaaat ccaataatag acattctaag tcctataaat gccaataata
     5341 tacctgcaat atcctgaatt aagtaatagt attcaaattt catattcaat caactcttct
     5401 ctgagcagta tcaaatattt ctaactcttc aatttcatca tctgtgtatg taagcatagt
     5461 aaaaccagtt acaccataaa taaatgaaat tataggtgta atccaactta agaaacaata
     5521 tggaataaat tctagtgggc ttacaccaag agtctgagca cagaaaacag catttgaatt
     5581 ccatggtatt aatggaccac caagtgtagc tgtatcttct ataattctag atagattttg
     5641 tggttttaat ttaaattctt tgtatattgg agacattaga gttccgacaa atacagaagt
     5701 gaacatcatt gatgaactaa atgcatttgc aaagtaactt actataaaag tagacccaac
     5761 caatttagtt cttgattttc ctattttagt tataagaggt tgaagcagta cttctaagaa
     5821 tccagttttt tggagaatac cagcaataac gaaaactaca aatactagta aaacggtttc
     5881 agccatactc attattccac cacgatttaa tagtttgtcg gcatagacaa atccagtatc
     5941 tatagtaaat cctgatagca tgtaatttaa aacatcacct attttttctc cttcttgaaa
     6001 tactgctata ataagtccca tgatagctga acttagtata gatataatgg gtggcttttg
     6061 tagcaataaa agtaataata aaaatattat tggaatcatt gctactaaac caatatgaaa
     6121 gtttgaattt aatacatcct tgatttgatt tatttgaaca tagtctatag tattatttga
     6181 atacttaaat ccaattactg tatacagaac tatacatatt acgtatgcag gtcctgtggt
     6241 ataaaacata tgcttcatat gtgtaattac atctgtctta caaactgctg cagctaagtt
     6301 tgtagaatct gatagaggtg acatcttatc tccaaagaaa gcaccagaaa tgactgcacc
     6361 agctgttaaa ccaagaggaa tacccatact agtaccaata ctcatcattg ctataccaga
     6421 agttccaaga gtcccccaag atgtaccagt agcaacagaa gttaatgaac atacgattaa
     6481 tgttgtaagc agaaaatatt taggggttat tattgttagt ccactatata ctactgttgg
     6541 tattgtacca gcagctatcc aagctgctat tagtacacca acagaaagta taattaagtt
     6601 tgattgaaag gcatctttac caacttcaaa tccaaaagct tctatttcat cattagtata
     6661 accaagtttc atagctgcag gaactactat aagccatgaa agtaaaaaca ttgtagttat
     6721 agaagcatta aatacaacta taccaactga tgttagagct attattgcaa gcattactaa
     6781 caaggaatat ctaaatgaag gtatttttct ttctgtattc attttcatag tgcaaattct
     6841 ccttaaatat ttttagttaa ttgcattttt tattcttata agagcttctt caagcatact
     6901 tcttggacaa gctaagttta ttctttgata cccacttcct ccaatgccaa agctgtttcc
     6961 ttgatttaga gcaactttac ctttttgtac aagtatactt tctagttcct catcacttaa
     7021 tccaagtgca ctaaagtcta cccataataa ataagtacct tctggttttc taactttaag
     7081 ttttggcata ttttcattta tatattttat agcaaaatct atattagatt ctaaatattc
     7141 taaaaatgat tctagccaag attcaccatt attatatgaa gcttcagttg caactaagct
     7201 aaagcaatta tttctcttta tatcaattct ggtaaaggca tcatctaaca atttatagtc
     7261 tttttcatca ggtagtatta catatgatga ttgaagacca gctatattaa aagttttagt
     7321 tggagccata caagtaattg tatttttttc aaattcttta gaaatagatg ccataggaat
     7381 atgcttatgt ttttttaata taatgtcact atgtatttca tcagatatga tttttacatt
     7441 atgcttaaga caaatatctc ctagtttttt taattcatct tttgtccata ctctaccaac
     7501 tggattatga ggattacaaa gtatgaataa ttttacatct ttaatcttgt tttcaatgtc
     7561 ttcataatcc attatgtagt ttccattttc tagtttttgt aatgggctta taattagttc
     7621 tctattgtta ttttttacaa cactattaaa agggctgtaa actggttctt gtatcattat
     7681 tttatcattt gcttttgtga gttcatttat taaaagactt atagctggga taacacctgg
     7741 actataaatt aaccactcac tttttatttt ccagttatgt ctcctatcta gccagtttac
     7801 tatagattca ttgtaagaat ctggtcttgt tgtgtagcca taaatctctt gttctagtct
     7861 attttttaag gaatcaatta tacaaggtgc agccttaaaa tccatgtcag ccacccacat
     7921 aggaagtaaa tcattagttc catacttttt ttccatttct gaccatttag atgaaaaatt
     7981 attgcttcta tcaacaatct cattaaaatt ataattcatt gtaaatacct ccagtaattt
     8041 taattttatg ttaataatat taagcaaaca acgtgccaat attgataata aaaaatattt
     8101 attcacaagt taatatttaa tattaagtaa tttcagagca tagataagat aaaaatatta
     8161 attatactta aaactttgtg gaaaaggtta tttctaataa atttttaaat atatgaaaat
     8221 gtaattaaat aggttaaata ggtttaactg tttaacttgt tagacctatt tatatttttt
     8281 agaaattcta ctccttttga tgttataatt gaacctccac gaccaacaag tatgtctaca
     8341 agcccaaagt tttgtagttc taaaagcata tttctaatct gttgttctgt caaaaatcta
     8401 ttttcctcta aagctatttt acataagctt tttctaccta ttctttcttt taaatcataa
     8461 gcctttttta aattatccaa tataaatatg tattcatcta gtgacctttt aaaattatag
     8521 tcgtatttca tgatgttttt gtctttgcca atgttgaatt gatacttttt tatattatca
     8581 cttactttat tacaaacggt tctactataa tttgaatcta tagtatctaa tatatattca
     8641 ggtaaatcac atatttctat aatatctttt tccaagtaac agaagtattc tcctaagttt
     8701 cttaattctc ttacatttcc ttcccatcta tacattttaa agatttcttt tacttcctca
     8761 cttagaataa aattacatgg aatatcatat tttaatgaac caaaaatttc aaatatatca
     8821 gcaggtcttt ccctaagtgg atgtatttta aggggaagaa cattcaacct aaaatataaa
     8881 tctcttctaa acatattttt agatacaagt tcttttaaat ttctattagt agctgcaatt
     8941 attcttacat caatgtcaat gacattatta gaaccaatac gtcgtacttg tttttcttgt
     9001 atgactctaa gtaatttaga ttgagaattt aaatccattt ctccaatttc gtctaaaaat
     9061 atagttccat tgttggctaa ttcaaataaa ccaattttcc cacctttttt agcaccagta
     9121 aaagcccctt catcatagcc aaataattca ctttgcagta gattttcttg aaaagtacta
     9181 caatttactg cgacaaaagg accatctttt ctcctagaag cactatgaat ggattgagca
     9241 aataattctt taccagtacc actttcacca attataagga tagatgaatt tgattgagcc
     9301 attttattag caattttttt tgtattaata gtttgtatac tagaacctaa tatgtcatca
     9361 aatgtgtatt tactgatatt accagagttt actagttgtg ctcttaattt agcttgcttt
     9421 ttttctaatt gagaaaactt tgtcatcttt agtataaatc catcaaatac atttaattgt
     9481 atatgtttaa tctctaaatt tatatcaata tgatttattt taatcaattt ctcaaaaaat
     9541 ggagttttat tggtcaatat gctttgaaaa ttgatgtctt ttatgcaatc accaacaaat
     9601 gagtcaatca tttcatttgc atttatagac aaaatttttc tagccatatg attatagaat
     9661 tgaattattc catcgttatt tgtgcatata attccatcat caattataga tagcaacaaa
     9721 tcaaattgtc tttcaaactt atttgcatct aacaataact cacctgtact ataacttgta
     9781 ggaattattt tttctacgta tttttttatt aggtcatctt ttattagatg ttttaatttg
     9841 gtttttgtag caatttcaac aatacaactt aaatctatta ttctgtattt taaatctata
     9901 acttcacaat ttttaaactt aggcaagatt ttttcgccag gagttaggat tacagcatct
     9961 gtgggagtaa actcaatctc tggatagcag ggcaggatat ttatattgtc tattcctaat
    10021 ctatgtatta aagaaattgt ttctattgcc atatctttac ttaagttata tacataaata
    10081 attttatctt tttctaattt ggatatcata tcaatactgt tcttttcaaa tgtcaactta
    10141 ggaacaatga tttgagcatc atagtgagat aattttacta tttcatcata ttttaaataa
    10201 gcagatagga gtataagttt ttctttaaaa aactttaaat ttccctcttc aaatgagtat
    10261 aaatttatct ttagattatc acctaagaat tgccttattt gattttcata aatttctcca
    10321 gcgtcttttt ttaaacttac aatagctaat tccatttgtt tcattttaac ctctcataca
    10381 aatttgactt attttagatt acataataat taaattagat taaataatat tatttaatta
    10441 ttattataaa tttattatag actacacatt gaagtaaaag aagtagctaa aattattcct
    10501 tttatttttg ttttttgtat ctatttggta taatagtatt agagcgtata ataatttagg
    10561 caaaattttt taaggaagac tagaggagaa tataaaatga aaaaatgtct ttactgtaat
    10621 agggatataa agtcaccaaa taataaatta gctataaaat atgatgacaa tatgaatata
    10681 tatccatgtc gtgcagaatg taaggaaaaa attgaagaat atatagcaaa aaaacctaca
    10741 tataagttta taaatatatt agaggttctt ttaggtgtgg gcggaatatt ctgctttttg
    10801 agtaaatgga ctattgcagc ccaagttgta cttggaattg atgcactggt tatattttta
    10861 ttcccattgg caggatttaa aatgaatgga aagttaagta tggaacaaac taagaatcaa
    10921 agtaggtcta ttgcaatatc aattttggca tttatagtaa taataactgt attatatttt
    10981 aaacaagttg gtaagtagag cataattgta taagacatca ttagagtatg aggtgagttt
    11041 atggaagaaa gaaaattagc accaaacaat gagagggaag ctctcataaa aagattaaaa
    11101 agaatagaag gtcaagttaa gggtatacaa aagatggtgg aacaagaacg ttattgtgtt
    11161 gatatattgg ttcaaatatc tgctataagg tctgctataa atagagtagg cacaattatc
    11221 ttagagaatc atataaaagg atgtgtagca gaaagtatta aacatgatga tgctgataaa
    11281 actgaagaga tgataaaaga gcttatggat actataaata aatttacaaa ataaggaggc
    11341 ggttagttat gaatcaacaa ggatttatta tggaaattgt tgatgataga actgcaaagt
    11401 taaaaatgca aagacattca gcttgtgctg cttgtggaaa atgtgctact acaaattcag
    11461 cagagtcaaa agaaatatta gtagaagtag acaacacaat tggtgcaaaa gttggtgacc
    11521 atgtagaagt gagtatggat aatatgaatg tattaaaagc agctgtaatg gcttatataa
    11581 tacctcttat agcacttctt gtaggaacaa tagggactta ttatatattg ggggcaattg
    11641 gaatgacaac tggagttgaa gccataagtg gattagttgg aatttgtctt acaataatct
    11701 gttatttata cttaaagaag aatgacaaaa aatttagaga cagtagagaa tttataccag
    11761 taataacaag aatattaatt gacctttaag ataattataa ttaaaaaata gcaatgtact
    11821 ttttaaataa atactcaata cgtatttgta gagtttaagt acattgctat ttttatattt
    11881 ttattttgat tttctgtaag gaataaagtt ttttctgatt tttaaaagat tcaatgttct
    11941 tacaaaagaa gttacaacta atacaccaag tgctattgca gcacttagaa atagcgcatc
    12001 tgtacctttt gacatagcca aagaataatt tttctgaatc aaagacaaca ttgtattata
    12061 tagcccaatt cctggtatta gaggaagaat acctggaatt acgaacacaa tagctggttg
    12121 ctttagtttt cttgctaaag tctcactgca cgctgagacc aaagcagcag cgataaaacc
    12181 tgcaaaaact gcatttgtag taaaattaaa taaataaaca tatacaatcc aaccaattcc
    12241 gccagttact ccagctggaa gaagtagata tagtggagca ttaaagaaaa cagcaaatcc
    12301 agcagttgca acacctgaaa ataaaaaatg caaaatcata gacatatcag tcattagaaa
    12361 ggacctccta ttctatacca tgtatcaagg acaaaaccaa ctccagaagc aattgcaata
    12421 ataatcatag cagcatcaaa agcacgagaa attcctgata ttaaattccc tgatattaaa
    12481 tctctaatag cttttataaa agcaactcct gggagtagtg gcattatagc gccgactata
    12541 agcattttag ggtcatctaa aatgcctatt tcaactagta gaactcctga aagtgcaatt
    12601 aaaaaagaag atactaaaca ggaaaatgca gatattgcac ttatcttcat cgttttatca
    12661 taagtaattg cagcaaagat tgctattata aacgtaagta taaaatttaa tacataattc
    12721 cctccaagca aacaagcaaa acaagcagat gcaattcctg tacaagagta aaataaccat
    12781 aaaggataag gttttacatc ttggatttca taaagacgtg caatggcatt atcaatatca
    12841 tactctttgt cactaacaaa ttctcgagaa aaactgttta ataaagaaat cttatttaag
    12901 tttatacctc ttgattgaat cgttttcata aacgaaaagc catcaaattt ctcatctgaa
    12961 ataattatta ctgtaggagt tgtaaaaata tttacatgat aaaaccctct tgacctgcat
    13021 attctaagga cactgtcttc aaccctagat gtttcagctc cattttttat catcagttcc
    13081 cctacaaata gagctagttt tagaatattt ttcttatatt caagttcctc attttgattc
    13141 ataaatacac ctccaaatta aaaaacgtaa tattgaaatt atatcaaatt tttaagtaat
    13201 attccttaat atctatacaa atatattatc ttttttttgt ttcaaaatag tcacatttaa
    13261 attatatcca attaaaagaa ttagacaata ttatttatta tattttaact taaaaataaa
    13321 gaaagtaact atgttaaata tggaaaaatg gcaaaacact tgactattaa ttggtaaagg
    13381 actataataa taaattatca tattgataaa aacgaaaatt atgaaatgtc tgtaaaaata
    13441 aaatttaaat aataactagg aggagtaata tgtttgagaa cttgaaaaaa gcggaccctg
    13501 agatttatga aagtatgaaa agagagttaa aaagacaaca aagaaatatt gaattaatag
    13561 catcagaaaa catagtatca gttccagtaa tggaaactat gggaagtcat cttacaaata
    13621 agtatgcaga aggttactca ggcaaaagat attatggtgg atgtgaatac atagatgaag
    13681 ttgaaactct tgcaatagat agaatcaaaa aaatatttgg agcagaacat gctaatgttc
    13741 aacctcactc tggagcaaat gcaaatattg gtgtatattt tgctatgtta aaaccaggtg
    13801 atgttgttat gggaatgaat ttatctcaag gtggacattt aactcatggt gctccagtta
    13861 atatttctgg tcaatattat aaattttatg aatatggtat agataaagac agtggactta
    13921 tagattttga tgaagttaga aagatagcac atgaagtaaa acctaaaatg atagttgcag
    13981 gagcaagtgc atatcctaga gaaatagatt ttaaaaagtt tagagagata gctgatgagg
    14041 ttggagcatt gcttatggta gatatggctc atatagcagg tcttgtggct gctggtcttc
    14101 accaaaatcc atgtgaagtg gctgattttg ttacaacaac aactcacaaa actttaagag
    14161 gacctcgagg gggtgttatt ctttgtaaag aaaaatatgc taaagacata gacaaagcaa
    14221 tattccctgg tattcaaggt ggacctcttg aacatataat tgcttctaag gcagtttgct
    14281 ttaaagaagc tttaagtgat gagtttaaag aatatcaggt acaagttgct aaaaatgcaa
    14341 aagccttagc tgaagaactt attaaaagag attttaaatt gatttctgga ggtacagata
    14401 atcatttaat actattagat ttgactaata aaaatataac tggtaaagca gcagaaaaga
    14461 gattggatga tgcttatata acagctaata aaaatacaat tccatttgac ccaaatggtg
    14521 ctcttattac aagtggaata agacttggta ctccagctgt aactactaga ggtatgaaag
    14581 aagaagatat ggcaataatc gctgaggcta tagacctatg tttaacttat gatgaagaat
    14641 ctaaggctag aacattggtt gttggtttaa ctgaaaaata tcctttatat gaagaatata
    14701 atattatgga ttaattataa agaacaacta aataaaatac atttaaataa ttacaatgat
    14761 tgtgtatgta tttgaatata gttagatata aaaaaggtgt ctcaaaatga actttttagt
    14821 ttatgaggca cttttttgta tggaatcgaa catatttgtg ttatttaaat aataaaaaat
    14881 atggttatct ctatttttag atggtctctt tgtatttttt aattaaaact ttaagatttc
    14941 ttaagaatta tactaggttt ttctaaagat ttaaagtcta tacttataag tatagtaatc
    15001 atatgaaaag aaaagcttta acgaggaggg aatcttatga aggtattaat tcttacagga
    15061 aaatttggta tgggtcatta ctcagcatca aattctttaa gtgaagatat aaaagcaaaa
    15121 tttgataatt cagaaataat aattaaagat atatttgagt acattatgcc taactattca
    15181 gataaaatgt acaagacttt ttctattctt gtaaatcggg gaagtagtct ttataattta
    15241 ttttataaat gtgctgaaaa tggaaagaaa gatataaaat ttactttttc agattatttt
    15301 ttaaataaat tagatacatt acttcatgaa gtacaaccaa cagtagttat ttcaactttt
    15361 ccattttgtt ctcaattagt atcaagatat aaggaaaaat ataactcaaa tcttccttta
    15421 ataacatgta taactgatat aagtagtcac tcagaatgga ttagtaaaaa tacagactgt
    15481 tatttagtag ctagtaaatc tactaaggaa gaacttgttt ttaaaggaat agatgaatct
    15541 aagattaaag ttaatggtat tccagtaaaa aaagaattta aaagaataga acatgtaaat
    15601 cattcaacta aaaaaaatat tcttataatg ggtggaggat taggattact gcctaaatca
    15661 gaacaatttt acaaagaatt aaatagttta gaaggagtaa aaactactgt aataactggt
    15721 aataataaaa aaatgtacta taaactatat ggtagatatg aaaatataga ggtagtaggt
    15781 tacaccaatg aagtatataa atatatgaaa gattctgacc ttattatttc taaaccaggt
    15841 ggaataacac tatttgaaac aatttattca gaacttccaa tattggcatt taatccattt
    15901 ttacaacaag agatagataa tgctagtttt atattaaata atgaaattgg gagaatactt
    15961 ggtaaaaata aaaaatacta tgtagatgag ataaaagatt taatttatga tgatgctact
    16021 cttaaagaaa tgagcagtaa catgaaagaa ttaaaaaaac aatttgataa taatacctta
    16081 gagaatattt tattttcact tgatgaacaa ggagcgtgta gagaatgtat gtagtaggtt
    16141 taattatcgt tgttgcattg atttttttag tccattcaat tatacctaca tactataata
    16201 aactattaaa taaagaggta ttaaaaaata tggctggaga aaatgaaatt gcacttacat
    16261 ttgatgatgg tccagataag agatatacag aaaaattact agatgtgtta aaagaaaatg
    16321 atatacaagc tatgtttttt gtagtagcaa agaatgctga aaaagagcct gaaattataa
    16381 aaagaatgtt gagagaaaat catatagttg gtttacattc attggaacac agaaatgcat
    16441 ggctttatag ttatagttat gttaaaaaag attttattga aagtacaaat ataatgaaaa
    16501 atttaggtgt agatgtaaac tactatcgtc caccatgggg tcatactaat atattttcaa
    16561 attcatttgt taaaaaatat aatctaaaaa tgactttgtg ggatgtaatg gcagaagatt
    16621 gggagaaaga tagtacagta gacattatta taaacaagtt aatgagtaga acaaaagaaa
    16681 attctataat ttgtttacat gatgcaggtg aaaattcagg tggtgcagta ggtgcacctg
    16741 agcgtactat tgaagcttta aagatagcta ttccaaaact taaagccagt ggtttaaagt
    16801 tcgtaacacc agaaaggatg tagttatgat aagtaaaact gaaaagaaaa ataaatgcct
    16861 aggtagtgta gcttttttag ttttactaat gggcataaca ggatattttg tttttagagg
    16921 gcagtcagtt gaatcattga taaaatcatt aaaaggtgca agccctatgt ttatactaat
    16981 tggatttgca atgatgttta tatatgttgc ctgtgaaggt ataaatatat acttaggtat
    17041 gaaagcttta aaccaaaaga ctactctttt aaaatgtatg ggatatgctt ttattggatt
    17101 ttattttagt tctattacac cttcagcatc aggtggtcaa ccagcacaag tttattatat
    17161 gaaaaaagac gatataaata tatcatattc ttcacttata ctattagtca ttgttgttat
    17221 tcaccaagta gttatactgg catatagtgg aataatgttc attatggaac gtgagtttat
    17281 attaaataat gtaagtggta tgaacatttt actaatatat ggtgtaataa caaatgttgc
    17341 tcttgtcatt ggagtaattg caattatatt ttcaaaaaaa cttgtaaaca attttataat
    17401 atcaataaca aatttattag gtaagttaag aataataaaa gatgttgaaa gttcaagaaa
    17461 agttataata tctcaaattg aagagtatgt taaaggtgct caatatataa agcagaatcc
    17521 aaaacttgta gtccaaattt tagtcataac tattgtacaa ataacagcta tgtttttagt
    17581 tcctttcttt gtatacaaag cttttcatct atcaacatat actgtatttg agatattagc
    17641 gatacaatcc ttgcttaata ttgctgtttc ttcattacca ttgccagggg ctgttggagc
    17701 ttctgaaaat agttttatga cattatttaa gatatttttc cctggtcact tattagttcc
    17761 ggcaatgtta cttagtaggg gaataagttt ttatgcattt gtagctataa gtggattgat
    17821 atgtatagta gtacatgtaa aatcttctga aaaagtaaaa gcagtgaaaa aagttgctta
    17881 tgtaagataa tattatatag attattttta ttaatatgtc aatttgtgtg tatattttaa
    17941 ttaagcttta aacatattta caggagtaga aactattaaa acgtgtgaca ttgctaagct
    18001 tttgctaata aagtataata ttttatatag tttgaaatat ttaagaaata tagcaaaaaa
    18061 aggagcttgg tgatgaataa agagattcca aaaaaaattt tagtagctga tgataatcct
    18121 gagattaggg aaatcgtaga aatactttta actggtgaag gttatgaagt tattatggca
    18181 acgaatggag aagaagctgt aaacttagta gatagtacga ttgatttgat tattttggat
    18241 gttgtaatgc ctgtaaaaac aggatttgtt gcatgtaatg aaattagaga aaaaacgaca
    18301 gcaccaatat tatttttaac tgcaaaaact caagactctg ataaagttat tggattttca
    18361 gcaggaggag atgactattt atcaaagcct ttttcatatt ctgaactaat ttcaagagtt
    18421 aagtctctac ttagacgtta ttatatatac cagggaaagg aaaaggaaag agagccaaat
    18481 atattaaaaa caaaagagtt atgtgtcaat ttagatactc aagatgttac actaaatgga
    18541 gaacctgtgt ttttgacaac tatagaatat agtatattaa ttcttatgtt gaaaaataga
    18601 aaaaaagttt tttcgtctga aaatttatat gaaactattt gggatgagca gtatttttat
    18661 acagcaaata atacaataat ggttcatatt agaaatttaa gaaaaaagtt agaaaaagac
    18721 caaaaaaatc cacaatatat aaaaaccgca tggggaaagg ggtattatat tgattaacaa
    18781 aattaagcca tctaagttgg gagttatact attgatatat gtagtaatct cagctgcagt
    18841 ggctttcatt ttaaatatac cattttactc tgctgttgaa tctatagtaa aagatgtatc
    18901 agaagggaaa gtatatttta aaagagaagt aaatcaactt atggataatt ttcaaactta
    18961 tgtgaataac aatggtataa gagcagatga tggagatgat atagcaaaat ggaatagtga
    19021 taattggttt gtttctatga agatatataa aggaaataag gttttttatg atacattata
    19081 ctatcctaat gaagtacctg ataagagtga ggaaaatgag ttttatagca gtcccatggc
    19141 tagaaactat gagatacaat tttctgatat taaagctaat atagttgtag tagctttatt
    19201 tagatttaga tttcaaaatt ttatagaagc aatttctatt acatttatgg cacttacatt
    19261 tattataaca tttttactgt tgtttagtag aaagataaag tacctaagga aaatagaaat
    19321 gggtataaag atagtagaga gtggcagttt aaagtataga ataccagtaa aaggtaatga
    19381 tgaattatca tcacttgcat ctagtattaa tgagatgagt catacattgg agaatcaact
    19441 tataaaagaa agggagatga agaataaaaa taaagatata gtaacttcaa tatcacatga
    19501 tgttagaact ccattaacgt cagtaatttg ttatttagat ttgattaaag accataaata
    19561 caatagtcct gagcaattgg aacaatacat aaaaaatgta aggtctaaag catatcaaat
    19621 aaaagattta acagatgacc tttttacgca ttttgttaat tctggagagg aagtttcaaa
    19681 agggtatgaa aaaataaatg ctaatgaatt tatttttcaa ttacttttag atagttcata
    19741 tacattggag gagaaaggat ttcatataac aatagaaaat gaactttata accaatatca
    19801 tatttatgta gatgttacac aatttagaag gatatttgat aatttatgtt ctaatataat
    19861 aaaatatgct aggaaagata agcctatagt atttttaatt aaagtagagg attctaaatt
    19921 actaattata caaaaaaata gagtaagtaa tttttctaag aatgtagaaa gttttggaat
    19981 tggtataaag aattgtgaga aaatagttgc atatcataat ggaagcataa atgtaaattc
    20041 agatgataaa gaatttgcta ttgaaatatc tattccagca aaagaatcat aaaaatttaa
    20101 attatatatt gtgtaattgc tattatatat tgtgtaattg cttatgtaaa attctatgaa
    20161 gcaggaagca tgtgacttta gttatgggat gttcaatatt gtgtacctct aggcaagtaa
    20221 caagtttact tacaagacca tataatatta ttatcttaaa aaaaggggac ataataatat
    20281 ggttttgaca ggagcacatt atatttacat tctatttatg gtaattatat taattactat
    20341 gattatgaaa aaggatacaa tagtaccatg tatattaggt gtttttttta tggggttgtt
    20401 ctttgaaaaa aacatttttg gtgctataac agcagtgttt aactctttta ttatatcttt
    20461 aaatgagttg ggtccaatta tcttgattat agctattatg gttgcattat ctaaagccct
    20521 agaagccaat aatgctatac agtatatggt gagaccattt tcgagagtta taaaaaattc
    20581 taatacagca ttttttgtaa ctggatttgt tatgttagtt ctttcttggt ttttctggcc
    20641 aactccagct gttgccttag ttggagctgt atttttacct gttgctatga gagctgggct
    20701 accagcaatt ggtgttgcag tagcattaaa tttatttggt catggtcttg ccttgtctac
    20761 agactttgtt attcaaggag ctccaagtat aacagctggt gcagcaggtg ttgcagtatc
    20821 atatgttata aatgatggta tgattttgtt ttgggtgatg ggaatcgtaa ccatatctat
    20881 ggcattttat acattaaaaa gagatattaa caaaggaatg tttagagaag aactaaaagg
    20941 ttttgaaagt gaagaagtag aagagttcaa tggaaaatct aaaatagcaa ctattttagt
    21001 agctctggga tttcttgctg atataattgc tatgtatata tttgacctaa aaggaggaga
    21061 tgcatcagct cttttgggtg gaacagcagt atttttaata ataattataa atgttataaa
    21121 ttttggcaaa gatagtcttg aaaatgtatg tgagaatata attgatggtt ttgtgtttgg
    21181 gataaaaata tttggagcaa ttataccaat agcagcattt ttctatatgg gtgaagttgc
    21241 accattgaca ggtgtatttg gaaaagtatt agcaccaggt tcacaaggtt tattatcaga
    21301 tataggcata gctctttctc aaacaatacc acttaataaa tttgccgtat caggtataga
    21361 aactgtagtg ggagcaataa ctgggcttga tggttctgga ttttctggga tttcattagt
    21421 tggttcactt gcctctgtat ttggaactgc tataaatgca agtgttggtg cactagctgc
    21481 attaggacaa atcagtggta tatgggtagg tggaggctgt ctagttcctt ggggtcttat
    21541 ttcagcagcg gcaatttgtg gagttagtcc aatagaattg gcaaagcgaa actttgtccc
    21601 agttataacc ggattggttg taacaaccat agtagcaatg tttattattt aataaatcga
    21661 ataaatcttt aattgtataa attaaaaata gatgtgtagc tataatttat tataattgtt
    21721 ttactaattc taagccatac atctattttt attgatattt ttattagtat tctttaataa
    21781 aataatattt ttcattatac tatacataac atctaaatat aacatctaaa atactgttat
    21841 ctatatttaa tatcttgcca aattttttga tgttttattg ccctgtccca ccaacatata
    21901 taagctatca caagttttta cctatactat caccaaacaa aaattctaat tttaatttat
    21961 tattttataa cctatattat attaactata ttagtttgta gactttagat gtaattataa
    22021 atattaatac tccaagagtt caaattcata tcctagtttt cttgcatttg atataaaatc
    22081 atttaaaatt tctgtactag ttttggaaac tgcatgtagt aacaagatag aaccatcatg
    22141 tagattatcc attatttttt gcgttgcttc ttcatgtgat ggttgtttat cagtatccca
    22201 gtctctgtat gcaaaactcc aaaatacagt cttgtatcct aaatttttag tcattgccaa
    22261 gcttttttct gagtattttc ccataggagg tctaaaaaat ttaaccatat ctttcccagt
    22321 tacatcttta tataactttt caacatcata taattcgtca ttaaagtttt ttagattgct
    22381 tgtttttgtt ggcatagatg gatgggtttt actatgattt ccaactatat gtccctcaga
    22441 aaccattctt tttactaaat ctttattttc ttttatatat ggagctgtaa caaaaaaaac
    22501 tgctttaacc tgattttgtt ttagagtgtc gagtattttt gtagtgtatc cattttcata
    22561 accttcatca aatgtaagat atagtgtttt ttgaccatct tttgtaggtc cattgtatag
    22621 ggcatcatag tcagaaaatt taaattcaat gtccgtattt acttctggtg ttttatgctt
    22681 attgtttggt atataaaacc aatcaagagt agtgttatct aaagaagata catctaattt
    22741 attattatca ttattagtag tggcttttga tggtttatct gttccgtctg aaactattac
    22801 attggaagta tctttaccac tatctatttt ttccttatct tcttgtaact gtgtttcctt
    22861 attttgattt tcgttttgat tgttttgtga atttgaacat ccaacaataa aaaaaataga
    22921 aatagaaagt agtgttaaat aatgcaatac ttttctaaac attttgaagc tcctttctaa
    22981 attaataata attgcatata ataataatta tatgattaat tacaattgca tatagtatta
    23041 ttataattgg aaattactat taataatagt tatatcttta aaaataaggt cacaatagta
    23101 tgacaaaggg tgaataatca ataaatttaa aaaaaaatga aaaaaatagt tgaccatatt
    23161 atgagaagat gttattataa taaatgtgct caacaagaag taacaaaata acaagcacaa
    23221 agaagttaat tgaat
//
LOCUS       DAYAPA010000058        23008 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.058, whole genome shotgun sequence.
ACCESSION   DAYAPA010000058 DAYAPA010000000
VERSION     DAYAPA010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23008
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.058"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(152..916)
                     /locus_tag="ACGI59_002072"
     CDS             complement(152..916)
                     /locus_tag="ACGI59_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFG9739858.1"
                     /translation="MFAEERIQAILNILKKEGRVTVKELSSKFNVTEDCIRKDLKNIE
                     KISSIRRIYGGAVLARETLENQDTKDRKEINIPTKKIIAEKAFNLIEDRETIFLDIST
                     INILLAKLLAESNKKVTLVTNMLDILNVVTSKPNNLNIISTGGLLNLSLDGFVGTPTI
                     EFISKYKFDKTFMGSCGVDVFNSSLTTFEIEDGLTKKAIINSSKKVFIVMEDKKFKFD
                     GNFKFAHLEDISSIITEKTPTPDIVDILSEFNVNVL"
     gene            1190..1864
                     /gene="fsa"
                     /locus_tag="ACGI59_002073"
     CDS             1190..1864
                     /gene="fsa"
                     /locus_tag="ACGI59_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFG9739859.1"
                     /translation="MQLILDTGNVEEIKELCTCLPIDGVTTNPSIVSKEKKNFKQLIN
                     EIGEIIGEDMPIHAQVLSTKYEEILEEALYISSLRKNIYVKIPVTQDGLRAIKDLHRK
                     GVKITATAIFTAHQGFLAAKAGANYIAPYVNRLDNISGDGVAMVSELIKIIDTYKMDT
                     KVLAASFKNAQQVIELMQHGVHSVTVPYDICKSMMNHPLTDWSVGKFIEDWENTFGKG
                     SKTNNI"
     gene            complement(1986..3380)
                     /locus_tag="ACGI59_002074"
     CDS             complement(1986..3380)
                     /locus_tag="ACGI59_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFG9739860.1"
                     /translation="MGIILKPKQFYMTVIATIILYFSLNYSFNIKFSICLSFLTILIW
                     AVDSVEKTFVALFFVILAFIFNIAPIKVILQFLFTENFYIIILAYIITNAVTKTGVAK
                     IISEKLILNTVNTPKKMILLSYILGILLIFFIPQPFPRVILVSAFYKEFLKEQQITED
                     SKSILLFSIFTASTFTSMFFVNGDTLLNYVVLELGNCNINWGQWAFYMSVPTIITCFI
                     TYFLFIFVFKKELHSIKFISNENLEGNKLHFSYSNLKKEFNLLNKEQKHVFLCLGIMF
                     FMFLTQFIHNINTLIIMSICVLLLLLRKIVGFSTLKEINWKVLIFFIAAFSIGGVLKY
                     SGVVDIMGNYLIKFIPNSSEIISILFLITLTIILNICLGSAVTTSSVVIPLLGSLHIL
                     KENSVTLCLFVYIIVSIQYILPFHHATIMVGHGENLYNSKVIFKYGLTLIPLTYFIII
                     CITFPWWKFIGLEI"
     gene            complement(3458..4804)
                     /locus_tag="ACGI59_002075"
     CDS             complement(3458..4804)
                     /locus_tag="ACGI59_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFG9739861.1"
                     /translation="MKDLTTGHEGKSIFFFAMPMLIGSLFQQLYNTADSIIVGRFIGK
                     EAMAAVSGANPIMFLLVAALMGVSLGFSILVSQFYGSGDLKKVKATIDTTYILLFIGS
                     ILISILGIVFGGPMLKLMNTPESVFAQSKLYLTIIFSGILFSAGYNSVSAILRGLGDS
                     VTPLYFLIIATILNIVLDLTFIVVLRMGVEGVALATIMAQAVSFIISIIYLNKKHEVL
                     KFKIKGIVYDNKIFKDGLRLGLPSGVQQMLFSIGNMALQFLVNSYGTSAMAAFGAGLR
                     IENFISLPIMNLGSAVSTFVAQNIGAGENERVKKGIRESIKMTLVLAVTVIALILLFR
                     ENLIALFNTDKDVIKIGSSYLFIIGPFFLFIGTSFVLSSAMKGAGDSMFALISSIVSL
                     WLGRLPASYMLSKFFGTDGIWMGIPFGWTLGLIVTVIYYKKGHWKTKAIVNHRINE"
     regulatory      5054..5156
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            5250..6626
                     /locus_tag="ACGI59_002076"
     CDS             5250..6626
                     /locus_tag="ACGI59_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFG9739862.1"
                     /translation="MQTTNSSTMQKLFPILTNKNVDMKKEIIAGVTTFLTMAYIIAVN
                     PNILSETGMPAGALVTGTCLAAAFGCILMGVVANLPFALASGMGLNAFFAYTVVLQMG
                     VPWEVALTAVFVEGIIFIVLSVSGVREAVVNAIPKNMKLAVTGGIGIFIALIGLVNSG
                     IVIGDQATLIKMGRFTPAVIITCVGLIIIAVLDKKRVKGSILFGIVVSSLLAWAFAFI
                     NPEYAQKLGIYLPGGLFKFESLAPIAGKIDLGYVLHPTNIGGFLVVVCTFLFVDFFDT
                     VGTLVGVCSKANMLDEKGNVPNVGRALLTDAVATTVGAGLGVSTVTTYVESSTGVIAG
                     GRTGWTAITVGILFLAAMFFSPVFIAIPSCATAPALIYVGYLMLGTVKDIEFDNITEG
                     VPAFVTIACMALTYSIGDGLTLGILTYVFVNIFYNIFGAKKAEDKKHVSVVMIVLAIL
                     FIIKLWLL"
     gene            complement(6740..7885)
                     /locus_tag="ACGI59_002077"
     CDS             complement(6740..7885)
                     /locus_tag="ACGI59_002077"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFG9739863.1"
                     /translation="MNTFEQLKISSTLIDGLKKQDITSPTEVQSLVIGNIIQNKDLLI
                     NSQTGTGKTLAYLLPIFEKIDTSKRETQALILAPTHELVMQITNQVELLAKNAELSVT
                     SLALIGEVNIQKQIKNIKAVKPHIVIGSCGRVLDLIKQKKLKSHNIKTIILDEVDNLL
                     NGKNITCIEDIIRTTLRDRQIIGCSASLTDSTIKICDKLMKEFEIIKTKEKSQINPNI
                     NHSYLLGEIRDKFTFLRKALAATNPKKAIVFVNNEKNIEVLVSKLNYHNYKAIGIFGN
                     MEKEDRKNAINKFKLGKAKILITTDLSARGLDIVDVSHVFNLDFPKSKNEYLHRCGRT
                     ARGNRSGNTISIITKKELDIIKDLQKEFNIVITPKTLQNGELIDIIK"
     gene            8138..8845
                     /locus_tag="ACGI59_002078"
     CDS             8138..8845
                     /locus_tag="ACGI59_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9739864.1"
                     /translation="MLKVINVSKSYKNKKVVSSVSFDVNDGELFGFIGHNGAGKTTTI
                     KAIVGIHDFEEGEVLINSKSIKKQPVECKKEMAYIPDNPDLYESLTGIQYLNFIADIF
                     EVAKDKREELIKYYSNKFELNKALGDLISSYSHGMKQKLAIISALIHSPKILILDEPF
                     VGLDPKASFILKEIMREFCDKGGCIFFSTHVLEVAEKICDKIAIIKDGNIIAYGTTEE
                     VKGSNSLESIFMELIEK"
     gene            8892..10487
                     /locus_tag="ACGI59_002079"
     CDS             8892..10487
                     /locus_tag="ACGI59_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9739865.1"
                     /translation="MSNLSILIKTNIINEFKLNALKKADGREKQKIMGMMLSVVIIAG
                     LLIFYVTSLSLSLVDILKQINQLEMLLIFGFGLSTLTTIVTSLYKTSSYLFQAKDLDL
                     LTSLPIKESTVLASKILMLVGANYLFSSICMIIPAVVYFLNSDVSITYFPYLILLFLA
                     LPLLPIVASSIIFLFIGQISSRIKYKNLVLIIGSIILMLLYTLLMSRLNSMSVEMLKN
                     SVSISETIRKVYPPIYYFVDALKTGSIVSFLAFIAISIIPFFMFVTLFAKKFKDINAK
                     MSEGYKSKNYEFKGQKTSRPSKALLKKEFKRYTSSFIYFLNSSAGLLFLMVGTILILL
                     FGADKVGELLKLNIDFNLIKIQLLGVFFFIIVMNCTTYCSISLEGKNLWILKSSPIDE
                     REIFWSKILMNFLLNAPLSILCLILVSIRLDFEIQFIIVSICLIVSFSMFVALMGLLS
                     NLLYPNLDWKNEVAVVKRSASMIVTMLVSLAYVIILGGIYVLLGISFLNIYIILASIL
                     TLILDFMIWRIISTKGVRIFKNL"
     gene            10590..11672
                     /locus_tag="ACGI59_002080"
     CDS             10590..11672
                     /locus_tag="ACGI59_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HFG9739866.1"
                     /translation="MRKLSKREKDFIQYGFLLFLICITTYLVLTTLDIKLIPKIIRLV
                     NKTYIFLGILTIIVYMIIESIVTHLIINAVQKTKVKFIGIKMATMGFYYNLVTPFASG
                     SQPMQIYALTKYDVSLSKSVAIVTNKTVVFQSTVTVFCGILILLNKSLLMEQVHSVKV
                     LITFGMIMNVSMLLGGLLIVFSPKQVKMIAEVLINFLSKFKTFKFLDSKRDRINHYID
                     DYNYSIKIFIKNVRILVLSILLTVLQLIAYFSIVYCVYKASNLRGVSYVHIMTLQVFL
                     YMAISPIPTPGNVGANEVTFFTMFSNIIPKELLGYSVLLYSGFVYYFILIVSGLFTFG
                     THYTLNSWKKENKVNSNLDKVIEKLN"
     gene            11821..11982
                     /locus_tag="ACGI59_002081"
     CDS             11821..11982
                     /locus_tag="ACGI59_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III"
                     /protein_id="HFG9739867.1"
                     /translation="MKQDKQNEKQSEISKIKQSNDNKLEIAQELHVSKNIGNMLGYRI
                     DKKKMNRPS"
     gene            12323..13591
                     /locus_tag="ACGI59_002082"
     CDS             12323..13591
                     /locus_tag="ACGI59_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFG9739868.1"
                     /translation="MEASIHTIASNNLLIIFAIVSITGIICSKLSEIIKVPDVVLYLL
                     VGILIGPSFLKFIDIRGFQIENQLILTFGSAFILYLGGKEISLKVLRNVKISVFLLAT
                     LGVVISAFIMQQVIGFTFGISAMTALLAGTIIASTDPATLVPIFNQVKIKDRVKQTVI
                     SESAFNDATGAILTSAVLAVILSNKFSLGENIYELGTMVIVGVLVGLITGILLLKLVN
                     DKPYGIFKDFAPIISVISVVVAYELSTKLGGSGYMSCFIVGIITGNKKNFKIWLSQKS
                     YDADFYVAETLGTICRMAIFIILGSQVNLAQLSKYFMPSVITVLVLMFIARPLCVLVC
                     TLVDREAKWSKSEILFMMWVRETGVIPAALCGIISAMKVPGYEVISSVVFMTILITLV
                     IQGSTTKLVAKKLGLLEEEVINISEKVSTF"
     gene            13786..14574
                     /locus_tag="ACGI59_002083"
     CDS             13786..14574
                     /locus_tag="ACGI59_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HFG9739869.1"
                     /translation="MFTVMDIVQPDTVEEAYSILNKRKTNQVIGGSAFLRMGKKRIGT
                     GIELSKLNLDYIKEYEDYVEIGSMTTFRTLETSSIIKNNFGRIIEDSVKNIIGVQFRN
                     VVTVGATVFSKYGFSDLIVALLSLDTEVELYNIGRISLEEFLNRDYEKDLLIKIYIKK
                     TNKNASYKSIRNAKSDYPILNVSVSKHMEQFKLCVGARPQKATIAKQASEFLSNNEIN
                     EINIDKAVEIASEELTFGSNMRASREYRKAMSKVLLKRAIMEVI"
     gene            14574..15023
                     /locus_tag="ACGI59_002084"
     CDS             14574..15023
                     /locus_tag="ACGI59_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HFG9739870.1"
                     /translation="MLVKININGKKKSVDIEPEEYLVDTLRKVGNLSVKRGCDTGCCG
                     LCTVLIDKKPTLSCATLTLRALDKEITTIEGLENEVKEFSEVLVKEGAEQCGFCSPGF
                     ILTVLAMKDELENPTDDEIKHYLTGNLCRCTGYMGQLRAIKTYLEVK"
     gene            15027..17291
                     /locus_tag="ACGI59_002085"
     CDS             15027..17291
                     /locus_tag="ACGI59_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HFG9739871.1"
                     /translation="MNIVGKGIKKIDGMSITTGKPVYTDDLSAKDALVVKILRSPHAY
                     AKIKDIDTSKAEMVDGVECVLTYKDVPKSRFTLAGQTYPEPSPYDRLILEDVVRYVGD
                     EVAIVAAIDEKTAIKAMNLIKVKYEVLTPVLDFEEAIGNKTMVHDEDRHCNFDIGMQR
                     EKNIVSHHDYTKGDVEKVLSNCDVVIEETYYTQAQAHAMMETYRAYNYLDHMGRLVVV
                     SSTQIPFHVKRHLSRALEIPASKIRVIKPRIGGGFGGKQTACVEIFSAIVTLKTGKPA
                     KIIYDRKETFNCSTSRHAMRLKVRLGATNDGIIKAIDIKALSDTGAYGEHASTTIGLV
                     GEKTLPMYNKVEATRFIADVVYTNKMPAGALRGYGASQGCFAVESTINMLANKLKMDP
                     TDLRLKNVVTEGETTFAYGKTLNSVDLIKCINKGKELIGWNEKYPCKHLDNGKIRSVG
                     MAITMQGSGIQGLDKSSAEIKLNDCGDYTLMIGSTDMGTGSDTILAQMAAEVLDTSIE
                     NITVISADTDLVPYDPGSYASSTTYVTGMAVVKAAQDLREKIISAGATKLGVEKDEVE
                     FDGIKLTSKKGSTLLFDIAVDSTIGPDTNQLIGLASHGSPVSPPPFIAGFAETEIDTE
                     TGKVDLVNYVAVVDCGTVINKNLAKIQVEGGIVQGIGMALFEEVRYTEKGSMDTNTFM
                     QYKIPARCDIGNVKVDFVETYEPTGPFGAKSVGEVVINTPLPAIQEAVFNGIGVRINT
                     LPITPEKVFMEMNK"
     gene            17431..17742
                     /locus_tag="ACGI59_002086"
     CDS             17431..17742
                     /locus_tag="ACGI59_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9739872.1"
                     /translation="MAGNLNNMRAVNNFRGDKNILECLVSFEGRSISQRKVRVFFKEK
                     QNQIEIDFAEEEISKLVENVVLNTSYQEMLYDEIEKQLEIDCIGTWMILSKLKDGSRV
                     H"
     gene            18028..18528
                     /locus_tag="ACGI59_002087"
     CDS             18028..18528
                     /locus_tag="ACGI59_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFG9739873.1"
                     /translation="MRKNSINIALTAAITAIYAVLTLSLGFISYGPIQFRVAEIMMLL
                     AFLDKGYIVGLTLGCFLANVIGPYGVPDIIFGTLATFISASMIYITRKLGKQNKSTLV
                     IASLWPTVINSIVVGLMLNVFFGLPLILSMIQVGFGEFVVVTIVGVPFFCFLMKKYKN
                     VINIKF"
     gene            18585..19571
                     /locus_tag="ACGI59_002088"
     CDS             18585..19571
                     /locus_tag="ACGI59_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFG9739874.1"
                     /translation="MSKVKRLTDVWIYINDKQKFTIKELSEEFNLSAKTIQRYLKELN
                     KMGLPIQAEQGRNGGYRVLNNSYIPPVIFTEKEVMIILFALKSLQIYNYKLMEIEINS
                     IMRKVSYERKSSIKVGIENIKRYIGFVANDTNDGDFKSSNVTEFFRASSESLILNIKY
                     KCGNQILEKNVCPIGIYLNKGAWFSPAYDCKSDKIILVHIDNIIDIKRTGESKKIHIN
                     LEEWLHEVYNNLYGNSSTTKKFGEIIYLNVLLTKEGVSKIKDSSWNLDMELNEDGSGI
                     ISTFIKVEDIDCYASILFFIGNNAKVIEPKFLNKLLYDKANELRYFYEENMV"
     gene            19621..20343
                     /locus_tag="ACGI59_002089"
     CDS             19621..20343
                     /locus_tag="ACGI59_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein"
                     /protein_id="HFG9739875.1"
                     /translation="MENFNKNAKIIGIGAEGINIINEVEEKIKANMDIEKININQEIE
                     KEYVRSLLDGVDILFLIYSSEDKHIRDIIKAVSYMSNERRVLSIGMDCSEKENKEDLE
                     LGREFKINNDSIFKFVDLMNIMVESISDSCMINIDITDLKEAIVGDKGIKYSFEEFED
                     TKSYSEIADILFDRMEYIGEEFISKKGIVFVEGSPEFSIMELNDLISNIQSKVEESYE
                     VIFSLYIKENLNGNIRVGLLYN"
     gene            20372..22306
                     /locus_tag="ACGI59_002090"
     CDS             20372..22306
                     /locus_tag="ACGI59_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFG9739876.1"
                     /translation="MSENIRGTEYWLMEEFFYQETDTPQIFREESIIALGFDIKINLK
                     KVFSMGKKSEKYINENCNDKDFIKKFMSIKKGDYFLYKAYQNIEEKVKCNVSIGIVEE
                     DFENGYELSHILGHTLPVKWMHNFDTVSNCKVYTSEFYKLNNNDISSYLEIIKSQDKK
                     LLRKRIEESENNKWYLKNYYYKNVDKCVDDEELFIQPINLEQLESLKNIKTDDYILLY
                     NSEKVICENDHTMKSLFGVCQVMEGFDKEKVSALEGGYFFPVRWIEYEKENIDGNAWV
                     IFENNSIVEKYVKRFTNKMPLNTILYGPPGTGKTYNTKQMVYNLVSETSLRNRYYTGY
                     LDYKNNVEFCTFHQSYGYEEFIEGLKSDGEGNFKPEDGILKDISIEACYNALKFEKKM
                     SFELEKDSLTLETIKDRKKKLVLENIEYSESFNFEESENYVLIIDEINRGNVSKIFGE
                     LITLLEDDKRLTKSNQTIVKLPYSKEKFSLPPNLYIVGTMNTSDKSIALLDIALRRRF
                     NFEEIMPDYDLLSIVDGIDIRKMLYTINKRIEFLYDRDHVIGQAYLLAVENINDIVVV
                     FRSKIIPLLQEYFYYDSEKVGLILGGIGRNEKDKYIVYKEKLIASELFKNNNASIMES
                     KVNYCIKQNISSEELKNIYE"
     gene            22299..>23008
                     /locus_tag="ACGI59_002091"
     CDS             22299..>23008
                     /locus_tag="ACGI59_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9739877.1"
                     /translation="MSKNIYVKETYEWIRIGNGENELTEIEYEKLLKYLENNNDVLKS
                     NIIDIKYKKLRFINYVGIICFENVILEILPKLSLSDNLVKDREILLQMLSICNKIPIT
                     MNEKIRLSLKNYNLLNFFVMYFIESMQTQMKKGIYFEYINKIENLNVMRGKILLSTYA
                     KEKGISPMKIRCEYDEYSENNFLNQVLKKACISILCRINDNSIQGKIKKILSYFQNVD
                     LIYIDRKKLLDYKFYKNN"
BASE COUNT         8822 a         2468 c         3727 g         7991 t
ORIGIN      
        1 atagacataa ggataaagta gatgttatag aactagattt taaaaaatag atactaagga
       61 taaaataaat attatagaaa tatgttttta agaatattta tcaaataaaa atttatattt
      121 ataagaaatc ctataaaaca tataattaaa cttataaaac atttacatta aattctgata
      181 aaatatctac tatatcagga gtaggcgttt tttctgtaat tatagaacta atatcttcta
      241 aatgagcaaa cttgaaattt ccatcaaatt taaacttttt atcttccata actataaata
      301 cttttttact tgaatttatt atagccttct tcgtaagacc atcttcaatt tcaaaagttg
      361 taagtgaaga gttgaataca tctactccac aacttcccat aaaagtctta tcaaacttat
      421 atttcgatat aaactctata gttggagtac cgacaaaacc gtctaaactg agatttaata
      481 aacctccagt ggaaattata tttaaattat ttggttttga agtaactaca tttagtatat
      541 ccagcatatt tgtaacaagt gttacttttt tattactttc agctaacaac ttcgcaagca
      601 agatgttaat tgtagagata tctaaaaata tagtttctct atcttcaatt aaattaaatg
      661 ctttttctgc tattattttc tttgttggaa tattaatttc ttttctgtct tttgtatcct
      721 gattctctag tgtttctcta gctagtacag ctcctccata aattcttctt attgaagaaa
      781 tcttttctat attttttaaa tcttttctta tacaatcttc tgtaacatta aatttagaac
      841 tgagctcttt gacagtaact ctaccttctt tttttaggat atttaatata gcttggattc
      901 tttcttctgc aaacattttc tacctctttt cgcaatatag tattatagta aatattataa
      961 gataaaatac aatatatcaa ctattttaac atgaaataca aaattaattt aatataaaaa
     1021 gtatgttttt tctaactata tatttagaat ttagtaaata aaaaaattat aaaactaaga
     1081 aggaaaagta aaacaataat aaaaaataaa aaacaataag caaaatgttg aaaataatac
     1141 caaatcaata tataataaga ataaagaaag attaaagggg gatattttaa tgcaactaat
     1201 cttagataca ggtaatgtgg aggagattaa ggagttatgt acttgtttac caatagatgg
     1261 tgtaactaca aatccaagta ttgtttcaaa agaaaaaaag aattttaagc aacttataaa
     1321 tgaaattggt gagattatag gtgaggatat gcctatacat gctcaagtgt taagtacaaa
     1381 gtatgaagaa atattagaag aagcattata tataagtagt ttgagaaaaa atatatatgt
     1441 aaaaatacct gtaacacaag atggtttaag agctataaaa gaccttcaca gaaaaggtgt
     1501 aaaaataact gctactgcaa tattcactgc tcatcaaggt tttttagctg caaaggctgg
     1561 agcaaattat attgcacctt atgtaaatag gcttgataat atatctggtg atggagtagc
     1621 tatggtttca gaacttataa aaataataga tacttataaa atggatacaa aagtacttgc
     1681 agccagtttt aaaaatgcac aacaagttat agaattaatg caacatgggg tgcatagtgt
     1741 tacagtacca tatgatattt gtaaatctat gatgaatcat ccattaactg attggagtgt
     1801 aggtaagttt atagaggatt gggagaatac ttttggaaaa ggaagtaaaa caaataatat
     1861 ttagtattat ggtaaaagag tagttttgtc caatttcaca agaacttata taaaaagatg
     1921 aaaatatgaa ttttcttttt aataatttcg ttattatctt aagttaattc atattttcat
     1981 cttttttaga tttctagtcc tataaatttc caccaaggga atgttataca tattattata
     2041 aagtaggtta aaggaatcaa agttaaaccg tatttgaaaa taaccttgct attatacaga
     2101 ttttcaccat gaccaaccat tattgtagca tgatgaaatg gtaatatgta ctgtatgctt
     2161 actataatgt agacaaatag acataaagta acactatttt cctttagtat atgtaaactt
     2221 ccaagtaacg gtattactac agatgatgta gtgacagcac ttccaagaca tatattcaag
     2281 attattgtga gagttatcaa aaacaatatt gaaataattt ctgaagagtt tggtataaac
     2341 tttatcaaat aatttcccat tatatctaca actccagagt actttaacac tcctccaatt
     2401 gaaaatgctg ctataaaaaa aataaggacc ttccaattta tttcttttaa agtactaaat
     2461 ccaactattt ttcttagtaa taaaagaagt acacaaattg acattataat taatgtgttt
     2521 atgttatgta tgaactgagt taaaaacata aaaaacatta ttcctaggca taaaaatacg
     2581 tgtttttgct ctttgtttag caaattaaat tcttttttta aattagaata tgaaaaatga
     2641 agtttattac cctcaagatt ttcattagaa ataaatttta ttgaatgcaa ttctttttta
     2701 aagacaaaga taaataaaaa ataagttata aaacaagtaa ttatagttgg aacagacata
     2761 taaaaagccc attgacccca atttatatta caatttccaa gttctagtac aacataattt
     2821 agaagggtgt ctccatttac aaaaaacatt gatgtaaagg tagatgctgt aaatatacta
     2881 aataaaagta tagattttga atcttcagtt atctgttgtt cttttaaaaa ctccttgtaa
     2941 aaagcagata caagaataac ccttggaaat ggttgtggga taaaaaatat taaaagtata
     3001 cctaaaatat acgataaaag aatcattttt ttaggggtat ttacagtatt taaaattaac
     3061 ttctcagaaa taatttttgc gacacctgtt tttgtaactg cattagtaat tatgtatgca
     3121 agaattatta tgtaaaaatt ttctgtaaat aaaaactgta aaataacctt tattggtgca
     3181 atgttaaata taaacgctag aattacaaaa aacaatgcaa caaaagtttt ttcaactgag
     3241 tctacagccc atatcaagat agttaaaaaa gataagcata tagaaaattt tatgttgaaa
     3301 ctgtaattaa gagagaagta gagaattatg gtagctataa cagtcatata aaattgtttt
     3361 ggcttaagaa taatacccat ttttatattc acctccataa tttattatat cataatggag
     3421 gtgaatacaa tgaatgtgaa tacattttat aaaaagatta ttcattgatt ctatgattta
     3481 caatagcttt agttttccaa tgtcctttct tatagtagat aactgtaaca ataagaccta
     3541 aagtccaacc aaaaggaata cccatccaaa ttccgtctgt tccaaaaaat ttagaaagca
     3601 tataagaagc tggaagtctt ccaagccata aagatactat tgagcttata agtgcaaaca
     3661 tggaatcacc agcacctttc attgcacttg aaagtacaaa agaagttcca ataaataaga
     3721 agaatggtcc tattataaat aagtaagaag agccaatttt aatgacatct ttatcagtat
     3781 tgaatagagc aattagattt tccctaaata gcaaaattaa agcaataact gtaactgcta
     3841 aaactaaggt catttttata gattctcgaa tgcctttttt aactctttca ttttctccag
     3901 cacctatgtt ttgagcaaca aaagtagaaa cagcagaacc aagattcata ataggtaagc
     3961 ttataaaatt ttctatccta agaccagcac caaaagctgc catagcagat gtaccataac
     4021 tattaactaa aaattgaaga gccatatttc caattgaaaa caacatttgt tgaactccac
     4081 ttggaagtcc taatctaagt ccatctttaa atattttgtt atcatataca attcctttga
     4141 ttttaaattt aagcacttca tgttttttat ttaaataaat tatacttatt ataaatgata
     4201 ctgcctgagc cattatagtg gcaagtgcaa cgccctcaac acccattctt agcactacaa
     4261 taaaagttaa gtctaaaact atatttagaa ttgtggctat tatcagaaaa taaagtggag
     4321 ttactgaatc tccaagtcct ctaagtattg cacttactga attatatcca gcagaaaata
     4381 atattccact aaatattata gtaagataca attttgattg agcaaaaacg gattctggtg
     4441 tattcataag cttaagcatt gggccaccaa atactattcc aagaatactt attaatatag
     4501 aaccaataaa taaaagtata tatgtagtat caatagtagc ttttaccttt tttaaatctc
     4561 ctgagccata aaattgtgac actaaaatag aaaatccaag gctaacaccc ataagcgctg
     4621 ccaccaataa aaacattata gggttagctc cagagactgc cgccattgct tcttttccga
     4681 taaatctacc aactattata ctgtcagctg tattgtatag ttgttgaaat aaactaccaa
     4741 taagcatagg catggcaaaa aagaatatgg actttccctc atgacctgta gttaaatctt
     4801 tcaccttatt cctccaaata aaaaaattat taatttatta tagtataatc ttaacataaa
     4861 ctttatatcc aaactataaa taaattgtat caaaatatca tatatttttt taaaatgtaa
     4921 agaatataat tataatttga atttaataaa tcaatattta atataatcga atattaatac
     4981 aaaatcaata caatatatac gtatatattg acaaaattaa ttatataaat taaaataata
     5041 ttcgtaaaga aaaattcatg ttttgtactg tataacatat aattttgaca atatgggtca
     5101 taagtttcta ccggaatacc gtaaatattc tgactatgta tactattatt tagttaatta
     5161 aatatttaat taactgaatt atgaaggata cggctcagct tatcaactac tgtgtcgttt
     5221 ttttgtaaaa tttaatagga ggtgttgtta tgcaaacaac aaacagtagt actatgcaga
     5281 aactttttcc aattttgact aataaaaatg tcgacatgaa gaaggaaata attgcaggag
     5341 taactacttt tttaactatg gcttatataa tagctgtaaa tccaaatatt ttatctgaga
     5401 caggcatgcc tgcaggtgca cttgttacag gtacttgttt agcagcagca tttggatgta
     5461 ttttaatggg tgtagttgct aaccttcctt ttgctttagc atcaggtatg ggtcttaatg
     5521 cattttttgc atatacagta gttttacaaa tgggtgttcc ttgggaagta gctcttacag
     5581 cagtatttgt agaaggtatt atttttatag tattatcagt aagtggagtt cgtgaggctg
     5641 ttgtaaatgc gataccaaaa aatatgaaat tagctgttac aggtggtata ggaatattta
     5701 ttgcacttat aggtctagtt aacagtggaa ttgttattgg agaccaagcc actcttatca
     5761 aaatgggtag atttacacca gcagtaataa taacttgtgt aggattaata attattgctg
     5821 tattagataa aaaaagagtt aaaggttcta tactttttgg aatagtagtg agttcattac
     5881 ttgcttgggc atttgctttt ataaatccag agtatgcaca aaaattaggt atatatttac
     5941 ctggtggatt atttaaattc gaaagtttag caccaatagc aggtaaaata gatttaggat
     6001 atgtactaca tccaacaaat ataggtggtt ttttagtagt agtttgtaca tttttatttg
     6061 ttgatttttt tgatacagta ggtacattag ttggtgtttg ttctaaagca aacatgttag
     6121 atgaaaaagg gaatgttcct aatgtaggta gagccttact tacagatgcg gttgcaacta
     6181 cagttggtgc aggacttgga gtatctacag ttacaactta tgttgagagt tcaacaggag
     6241 ttatcgcagg tggaagaact ggatggacag caataacagt tggaatttta ttcttagctg
     6301 caatgttttt ctctcctgta tttatagcaa ttccatcatg tgctacagca ccagctttaa
     6361 tatatgttgg atatttaatg cttggtacag ttaaggatat agagtttgat aatataactg
     6421 aaggtgttcc agcttttgtt actattgctt gtatggcatt aacatatagt ataggtgatg
     6481 gtttaacttt aggtatatta acttatgttt tcgtaaatat attttataat atatttggag
     6541 ctaaaaaggc agaagacaaa aaacatgttt ctgtagtaat gattgttctg gcaatattat
     6601 ttataataaa actttggcta ctataatagg tattatttaa aattttaatt taatataaaa
     6661 taaagggctg taatttatta tcaattgtga tgataaacta cagctctttt gtatttgttg
     6721 tatttttgta tttattgtat tatttaataa tgtctattag ttctccattt tgtaaagttt
     6781 taggtgtaat tactatattg aactcttttt gcaaatcttt tataatatct aattcttttt
     6841 ttgttattat tgagattgtg ttaccacttc gatttcctct agcagttcga ccacatctat
     6901 gcaggtattc atttttactt tttggaaaat ccaagttaaa aacatgactt acatccacaa
     6961 tatctagtcc acgagcagat aaatcagttg taattaaaat ctttgcttta ccaagtttaa
     7021 atttatttat tgcattcttt ctatcttctt tttccatatt tccaaaaatt cctattgctt
     7081 tgtaattatg ataatttagt tttgatacta ggacttctat atttttttca ttatttacaa
     7141 atacaatagc tttttttgga tttgtagctg ctaatgcctt tctaagaaaa gtaaatttat
     7201 cccttatctc acctaataaa tatgagtgat tgatgttagg atttatttgt gatttttcct
     7261 ttgtttttat tatttcaaac tccttcataa gtttatcaca aatttttata gtgctatcag
     7321 taagacttgc agagcatcct ataatttgtc tatctcttaa cgttgttctt attatgtctt
     7381 ctatacaagt tatatttttt ccattcaata gattgtccac ttcatctaag ataatagttt
     7441 ttatattatg tgattttagt ttcttttgtt ttatcaagtc taaaactctt ccacaagagc
     7501 caattactat gtgtggtttt actgctttta tattttttat ttgtttttgt atattgacct
     7561 ccccaattaa agctaaagaa gtaacggaga gttctgcatt tttagccaat aattcaacct
     7621 ggtttgtaat ctgcattact aattcatgcg taggtgcaag tataagagct tgtgtttctc
     7681 tttttgatgt atcaattttt tcaaatatag gtaaaaggta tgctagtgtt tttcctgttc
     7741 cagtctgtga attaatcaat aaatccttat tttgaattat attccctata acaagtgact
     7801 gaacttctgt tggagaagtt atgtcttgtt ttttgagtcc atctattaga gtggaactta
     7861 tttttaattg ttcaaatgta ttcatatttt actccttata ttttaataat tatagtcaac
     7921 tattttttat tatacattgt ttattcaaat cgaactatat attcttttat aattatgtgt
     7981 aatttgaaag taaatatcaa ttatatttat acctttatat ttctgatacc tttgcatttt
     8041 atagatttct aaaataaaca aataaaagtt gtaattgttt gatatttgtt atatattata
     8101 tataacaaat attaaaacta taatatggag ggaaattatg ttaaaagtaa tcaatgtatc
     8161 taaatcatat aaaaataaaa aagtagtaag tagtgtctca tttgatgtaa atgatggaga
     8221 gctttttggg tttataggac ataatggagc aggtaaaact actactatta aagcaattgt
     8281 tggaatacac gattttgaag agggagaggt actaataaat tctaaatcta taaaaaaaca
     8341 gcctgttgaa tgtaaaaagg aaatggcata tatacctgat aatccagatt tatacgagtc
     8401 attaactggt atacaatacc ttaattttat agctgacata tttgaagttg ccaaagataa
     8461 aagagaagag ttaataaaat attattcaaa taagtttgaa ctcaataaag cattaggaga
     8521 tttaatatct tcatattcac atggtatgaa acaaaaatta gctataattt cggctttgat
     8581 acacagccca aaaatcttaa ttcttgatga accttttgta ggtcttgacc caaaagcttc
     8641 ctttatattg aaggaaatta tgagggagtt ttgtgacaaa ggtggttgta tatttttctc
     8701 aacacacgtt ttagaagttg ctgaaaaaat ttgtgataag attgccataa taaaagatgg
     8761 aaatataatt gcttatggaa ctacagaaga agttaaggga agtaattcac ttgaaagtat
     8821 ctttatggaa ttaatagaga aataaaatgt tttttataca atatagtgtc taaagtgagg
     8881 aatgataaaa tatgagcaat ctaagtatat taataaaaac taatataata aatgaattta
     8941 aattaaatgc gttaaaaaaa gctgatggta gagaaaaaca aaaaataatg ggaatgatgc
     9001 tttcagttgt tataatagcg ggtctattaa ttttttatgt aacaagctta agtctctctt
     9061 tagttgatat cttaaagcaa ataaatcaac tggaaatgct actgatattt ggttttggat
     9121 tatcaacatt aacaactatt gttacatcat tatataaaac gtcatcatat ctatttcaag
     9181 caaaagattt agaccttttg actagtttac ctataaaaga atctactgta ctagcaagta
     9241 agatattaat gttggttgga gcaaactatt tattttcttc aatatgtatg attatacctg
     9301 cagtggttta ctttttaaat tctgatgtaa gtataactta tttcccttat ttgatattgc
     9361 tatttttagc tttaccatta ttaccgatag ttgcttcatc aataattttt ctatttatag
     9421 ggcagatatc atcaagaata aaatacaaaa atttagtatt aatcataggt agcattatat
     9481 tgatgttgct ttatacccta cttatgtcaa gacttaattc tatgtcagtt gaaatgctaa
     9541 agaatagtgt gtctatttca gagacaatta gaaaagtata tcctcctata tattattttg
     9601 ttgatgctct taaaacagga agtatagttt catttttagc atttatagcc atctcaataa
     9661 taccattttt tatgtttgta actctatttg caaaaaaatt taaagatata aatgctaaga
     9721 tgagtgaagg atataagtca aaaaattatg aatttaaggg tcaaaaaact tcaagacctt
     9781 caaaagcttt attaaaaaaa gaatttaaga gatatacttc ttcgtttata tactttttaa
     9841 attcatctgc aggtttgtta tttctaatgg taggtacaat tttaatatta ttatttggag
     9901 cagataaagt gggtgagtta ctgaaattga atatagattt taatcttata aaaatacaat
     9961 tattgggagt attcttcttt ataatagtaa tgaattgtac aacatactgt tcaatttctc
    10021 ttgaaggaaa aaacttatgg atactgaaat cttctccaat agatgaaaga gaaatattct
    10081 ggtcaaaaat attgatgaat tttttattga atgcaccact atctatttta tgtctaatat
    10141 tagtatctat aagattagac tttgaaattc agtttattat tgtatcaatc tgtttgattg
    10201 taagtttttc aatgtttgta gcattgatgg gattattatc aaatttatta tacccaaacc
    10261 ttgattggaa aaatgaggtg gctgtagtaa aaagaagtgc aagtatgata gttacaatgt
    10321 tagtatcatt agcttatgta attatacttg gaggtatata tgtattactt ggaatttcgt
    10381 ttttaaatat atatattatt ttggcaagta tacttacttt gatattagat tttatgatat
    10441 ggagaattat ttctactaaa ggagtaagaa tatttaaaaa tttataggtg tatttatgtc
    10501 aaataaagac ttatcttttt tagtcttgca aataataggt taagtagtat actatgtata
    10561 gtgatatgaa aaaatttagg agaaaataaa tgagaaaatt atccaaaaga gaaaaagatt
    10621 ttattcaata tggattttta ttatttttga tatgtattac tacttattta gtattaacta
    10681 ctttagatat aaaactaata cctaaaatta taaggctagt aaataagact tatatatttc
    10741 ttggcatatt gacaattata gtatatatga taattgaatc tattgtaact catttgataa
    10801 taaatgcagt tcaaaaaaca aaagtaaaat ttattggtat taaaatggct acaatgggat
    10861 tttactataa cttagttact ccatttgcat ctgggagtca accaatgcaa atatatgcac
    10921 ttacaaaata tgatgtaagc cttagtaaat cagtggctat agttactaat aaaactgtag
    10981 tatttcaaag tacagttact gtattttgtg gaattttaat tttgctaaat aagtcattac
    11041 tgatggagca agtacattct gtgaaagtct tgataacttt tggaatgatt atgaatgtaa
    11101 gtatgttgtt aggtggacta ctaatagtat ttagtccaaa acaagtaaaa atgatagcag
    11161 aagttttgat taatttttta tctaaattta aaacatttaa atttttagat tcaaaacgtg
    11221 atagaataaa tcattatatt gatgattata attattctat aaaaatattt attaaaaatg
    11281 taagaatctt agttttaagt atattactta ctgtgttaca attaatcgca tatttcagta
    11341 tagtttattg tgtgtataaa gcctcaaatt taagaggagt ttcatatgta catataatga
    11401 cattacaggt ctttttatat atggctatat caccaattcc aacaccagga aatgttggag
    11461 ctaatgaggt tacatttttt actatgttct ccaatataat tccaaaagaa ttattggggt
    11521 actctgtact cttatatagt gggtttgtat actattttat attaatagtt tcaggtttat
    11581 ttacatttgg aacgcattat acattaaatt cttggaaaaa ggaaaataaa gtaaattcta
    11641 atttagataa ggttattgaa aaattaaatt aataaaataa ttagggaaat tctatatata
    11701 aatatggtgt tgaataagct atattatatt gagatatttc ccttttttga ttaaaattat
    11761 agaaatttga aataataagt aataaacaga aaaagtgata ttgttttgaa aggagaaact
    11821 atgaaacaag ataaacaaaa tgaaaaacaa agtgagattt ctaagataaa acaaagcaat
    11881 gataataaac ttgaaatagc tcaagaatta catgtaagta aaaatatagg gaatatgctt
    11941 ggatatagaa tagataagaa aaaaatgaat agaccaagtt aatgaaaatg ttgaaaatgg
    12001 tacattttaa aaaatatata taatgaaatg ttttattgtt aaatttaaac ttattttaat
    12061 gggggaaaaa gtcgaaaaat atctaaaaag atatggatat aaaaaatatt aatatatata
    12121 tactataaac ttgacaaaat caacctttta ataactagca aaaaatagca aaattatata
    12181 agcgttattt aattgtcaaa aatgaaagct aaattgcatg tcctaaaaaa aatgattgat
    12241 aacatttatt tgtgtgctat aattatatag taaaacaata agtatattgc ataatgtatg
    12301 caaatcggga ggagagacgg acatggaagc atctatacac acaattgcca gcaataattt
    12361 attgataatt tttgctattg taagtataac tggaataatt tgtagtaagc ttagtgaaat
    12421 aataaaggtt ccagatgtag tgttatattt acttgtaggt attttaatag gaccatcatt
    12481 tttaaaattt atagatattc gtggatttca aatagaaaat caactaatac ttacatttgg
    12541 ttctgcattt attttatatt taggtggaaa agaaataagt ttaaaagtat tgagaaatgt
    12601 gaagatatct gtattcttat tagctacttt aggagttgtg atatctgctt ttataatgca
    12661 acaagtaata ggatttactt ttgggataag tgcaatgact gctttactag caggaactat
    12721 aatagcatca acagacccag ccactcttgt acctatattt aatcaggtaa agataaagga
    12781 tagggtaaaa caaactgtaa taagtgaatc agcttttaat gatgcaacag gagctatact
    12841 aacatcagca gtgttggctg taattttgtc aaataaattt tcattaggtg aaaatattta
    12901 tgaactgggg acaatggtta ttgtcggagt tttagttggt ttaattacag gaatactgct
    12961 tttgaaatta gttaatgata aaccttatgg aatatttaaa gattttgcac caataatatc
    13021 tgttatttca gttgtagttg catatgaatt atctacaaaa ttaggtggaa gtggatatat
    13081 gtcttgcttt attgtaggta taataactgg taacaagaaa aattttaaaa tttggttaag
    13141 tcaaaaatct tatgatgcag atttttatgt tgctgaaact ttaggtacta tttgtcgtat
    13201 ggcaatattt ataatattgg gtagccaagt taatttggct cagttaagta aatattttat
    13261 gccatctgta attacagtat tagtattaat gtttatagca agaccacttt gcgttttagt
    13321 ttgtacactt gtagacaggg aagcaaaatg gagtaaaagt gaaatattat ttatgatgtg
    13381 ggtaagggaa acaggcgtaa taccagcagc actttgtggt attatttcag caatgaaagt
    13441 tcctggatac gaagtgattt catctgtagt attcatgact atactaatta cattagtaat
    13501 acaaggaagt actacaaaat tagtagctaa aaaacttgga ttattagaag aagaagtgat
    13561 aaatataagt gaaaaagtat caacttttta aagttttgct ttaaaatagt acctgttaat
    13621 ggctatttta atattgtcta atgatgatta ttgaaatagt cattttttta tgtataagta
    13681 atttacagaa tttacatatt agacatattt attatcaaaa tttgttgaag ctatgatata
    13741 attagttgta tttaaaataa gaatagcaaa taagggagga aatttatgtt tacggttatg
    13801 gatattgtac agccagatac agtagaagaa gcgtactcaa ttttaaataa aagaaaaact
    13861 aaccaagtaa ttggtggaag tgctttttta agaatgggga aaaaaagaat aggaacagga
    13921 atagaattat ctaaattaaa tttagattat ataaaagagt atgaagatta cgttgagatt
    13981 ggttctatga ctacttttag aacattagaa acaagttcca taataaaaaa taattttgga
    14041 agaataatag aagattcagt taaaaatata ataggagttc agtttagaaa tgtagtaaca
    14101 gttggtgcaa cagtattttc taaatatggt ttttctgact tgatagtagc attactttca
    14161 ttagatacgg aagtagaatt atataatatt gggagaatta gtcttgaaga gtttctaaat
    14221 agagattatg agaaagattt acttattaag atatacataa agaagacaaa taagaatgct
    14281 tcttataaat caattagaaa tgcaaagagt gattatccaa ttctaaatgt atctgtatca
    14341 aaacatatgg aacaatttaa gttatgtgta ggagcaagac cgcagaaagc tacgattgca
    14401 aaacaagcta gtgaatttct atctaacaat gaaatcaatg aaattaatat agataaagca
    14461 gtagaaatag catcagaaga actaactttt ggttctaaca tgagagcatc tagggagtat
    14521 agaaaagcta tgagtaaagt acttttaaaa agagctataa tggaggttat ttaatgttag
    14581 taaagattaa tataaatggg aagaaaaaaa gtgttgatat agaaccagaa gaatatctgg
    14641 ttgatacttt aagaaaagtt ggtaatttaa gtgtgaaaag aggttgtgac acaggttgtt
    14701 gtggtttatg tacagtttta attgacaaga aacctacatt atcttgtgca acactgacat
    14761 tgagagcttt agataaagaa ataactacaa ttgaaggttt agaaaatgaa gttaaagaat
    14821 ttagtgaagt tttggtaaaa gaaggagctg agcaatgtgg tttctgttct cctggcttta
    14881 tacttactgt acttgcaatg aaggatgagc ttgaaaatcc tacagatgat gaaataaagc
    14941 attatcttac aggaaatcta tgtagatgta cagggtatat gggacagtta agagctatca
    15001 aaacttatct ggaggtaaaa tagaacatga atatagttgg aaaaggaatc aagaaaatag
    15061 atggaatgag tatcacaaca ggaaagcctg tatatacaga tgatttatca gcaaaagatg
    15121 ctttggtggt aaagatactt agaagtcctc acgcttatgc aaaaataaaa gatatagata
    15181 catcaaaggc agaaatggtt gatggtgttg aatgtgtttt aacatataaa gatgtaccaa
    15241 aatctcgatt tacacttgct ggtcaaacat atccagagcc atctccatat gatagattaa
    15301 ttcttgaaga tgttgtaaga tatgtaggtg acgaagtagc tatagtggca gctatagatg
    15361 aaaaaactgc aattaaagca atgaatctta tcaaagtaaa gtatgaagta cttacacctg
    15421 ttttggattt tgaagaggca ataggaaata aaactatggt acatgatgaa gatagacatt
    15481 gtaactttga tataggtatg caaagagaaa aaaatatagt atcccatcat gattatacaa
    15541 aaggtgatgt agaaaaagta ttaagtaatt gtgatgtagt gattgaagaa acttattata
    15601 ctcaagctca agcacatgca atgatggaga cgtatagggc atacaattat ttagaccata
    15661 tgggaagatt ggttgtagta agttcaactc aaataccatt tcatgtaaaa agacatttat
    15721 ctcgtgcact agaaatacca gctagcaaaa ttagggttat aaaacctaga attggtggcg
    15781 gatttggtgg taaacaaact gcatgtgttg agattttttc tgcaattgtt actttaaaaa
    15841 cagggaaacc agctaaaata atttatgata gaaaagaaac ttttaattgt tcaacaagta
    15901 gacatgcaat gagattaaaa gttagactag gtgctacaaa tgatggcata ataaaggcaa
    15961 tagatataaa agcactttct gatacaggag catatggaga gcatgcttct acaactattg
    16021 gtttggttgg agaaaaaact ttaccaatgt ataataaagt tgaagcaaca agatttattg
    16081 cagatgtggt atatactaat aagatgccag caggagcact tagagggtat ggagcatcac
    16141 aaggatgttt tgctgttgaa tcaactataa atatgcttgc taataaatta aaaatggacc
    16201 caacagattt aaggttaaaa aatgtggtaa cagaaggtga aactacattt gcatatggta
    16261 aaactttaaa tagtgtcgat ttaataaaat gtataaataa aggcaaagaa cttattggat
    16321 ggaatgaaaa atatccttgt aaacatcttg ataatggaaa aattagaagt gtgggtatgg
    16381 caattactat gcaaggttct ggtatacaag gtcttgataa gtcttcagct gaaataaaat
    16441 tgaatgactg tggagattat actcttatga taggttctac tgatatgggt acaggaagtg
    16501 atacaatttt agctcaaatg gctgctgagg ttcttgatac aagcattgaa aatataacag
    16561 ttatatcagc tgatactgac ttagttccat atgacccagg ttcatatgct tcaagtacta
    16621 cttatgtaac aggtatggca gtagttaagg cagctcaaga cttaagagaa aaaataattt
    16681 ctgctggagc aactaaacta ggtgttgaga aggatgaagt agaatttgat ggtataaaat
    16741 taactagtaa aaaaggtagc actttattat tcgatatagc agttgattct acaattggtc
    16801 cagatactaa tcaattaata ggtcttgctt ctcatggtag cccagtatct cctccacctt
    16861 ttatagcagg ttttgcagaa actgaaattg acacagaaac tggaaaagtt gatttagtaa
    16921 attatgtagc tgtagtagat tgtggaacgg ttataaataa aaatctagct aagatacaag
    16981 ttgaaggtgg aattgttcaa gggattggaa tggcattatt tgaagaagtt agatacacag
    17041 aaaaaggaag catggacaca aatacattta tgcaatacaa gattccagct agatgtgata
    17101 ttggaaatgt taaagtagat tttgttgaaa cttatgaacc aactggacca tttggtgcta
    17161 aatctgttgg agaagttgtt ataaatacac ctttaccagc aatacaagaa gctgtgttta
    17221 atggaattgg tgtaagaata aatacactcc ctattacacc tgaaaaagtt tttatggaaa
    17281 tgaataaata attatttatg aaaatttaga ctagttttac atgttaaaca accagttatg
    17341 tcatacctgt tgaaagatat ttaataagta gttattataa tattatgaat tttacttata
    17401 aaatacaaaa ttaaataagg ggatgatgtt ttggctggta atctaaataa tatgagagca
    17461 gtaaataatt ttagaggaga taagaacatt ttagaatgtt tagtcagctt tgagggtcgt
    17521 tcaataagtc agagaaaagt aagggtattt tttaaagaaa aacaaaatca aatagaaatt
    17581 gattttgcag aagaggaaat ttctaaattg gttgaaaatg ttgttttaaa tacatcatat
    17641 caagaaatgt tatatgatga aatagagaaa caactggaaa ttgattgtat aggtacttgg
    17701 atgatattat ctaaattaaa agatggtagt agagttcact gattttaaaa taaattatta
    17761 gagcctaatt ttaaggctct tttttgctgt ttttaattaa caatagaaaa tttatcttta
    17821 gtttgtatat aattttaata tcatatattc tatatatata aaattgaatt tgtatgtatt
    17881 atattgtata atataaaatg aattgggaaa atagttcgct aaaataattt gtttttataa
    17941 aattactgtt taagtttata gattttgtat aaaataaagt tatgttatta atttttttaa
    18001 aaataaaact tttactagga gacagatatg agaaaaaata gtattaatat agcactaact
    18061 gcagcaatta cagcaatcta tgcagtttta acattatcat tgggatttat aagttatgga
    18121 ccaatacaat ttagggtagc agagattatg atgcttttgg catttttaga taaaggatat
    18181 atagtaggtc ttacattagg ttgtttttta gcaaatgtaa taggaccata tggtgtacct
    18241 gatataattt ttggaacatt agccacattt ataagtgcaa gtatgattta tattactaga
    18301 aagttaggta aacaaaataa aagtacttta gttatagctt ctttatggcc aactgttata
    18361 aattcgattg tggttggtct aatgttaaat gtattctttg ggcttccatt aattctatct
    18421 atgatacagg taggttttgg agaatttgta gtagtaacaa ttgtaggagt accatttttc
    18481 tgctttttaa tgaaaaaata taaaaatgtc attaatatta aattttaaat agtgtatagt
    18541 aatttaatta attgagagta ttattagtga aaagaggtgt ttatttgtcg aaggtaaaaa
    18601 gattgactga tgtttggata tatattaatg ataagcaaaa gtttacaata aaagagttgt
    18661 cagaggaatt taatctttca gctaaaacaa tacaacgata tttaaaagaa ttaaataaaa
    18721 tgggacttcc aattcaagca gaacaaggta gaaatggagg ttatagggtg ctaaataata
    18781 gctatatacc tcctgttata tttacagaaa aagaagttat gattatacta tttgcactta
    18841 agtcacttca gatttataat tataagttaa tggaaataga aattaattct ataatgagaa
    18901 aagtaagtta tgaacgtaaa agcagtataa aagtaggtat agaaaatatt aaaagataca
    18961 ttggctttgt agctaatgat actaatgatg gagattttaa gagtagtaat gtaactgagt
    19021 tttttagagc ttcttcagaa tctctaattt taaatattaa gtataagtgt ggtaatcaga
    19081 ttttggaaaa aaacgtatgc cctataggta tatatttaaa taagggagca tggttttcac
    19141 cagcatatga ttgtaaaagt gataaaataa tattagttca tatagataat ataattgata
    19201 taaaaagaac aggagaaagt aaaaaaatac atataaactt ggaagaatgg ttacatgaag
    19261 tatataacaa tttatatgga aatagttcaa ctactaaaaa atttggtgaa attatttatc
    19321 taaatgtatt attaactaaa gaaggtgttt ctaaaataaa ggacagttct tggaatttag
    19381 atatggagct aaatgaagat ggtagtggaa taattagtac attcataaag gttgaagata
    19441 tagattgtta tgcttcgatt ttatttttta ttggaaataa tgctaaggtt atagagccaa
    19501 aatttttgaa taagctactt tatgataagg ccaatgagtt aagatatttt tatgaagaaa
    19561 atatggtata atatttagaa atgttgaaat taaataatta ttaaggagat atacgttata
    19621 atggaaaatt ttaataagaa tgctaaaata attggtattg gagctgaagg tattaatata
    19681 ataaatgaag tagaagaaaa aataaaagca aatatggata tagaaaaaat aaacataaat
    19741 caagagattg aaaaagaata tgttagaagt cttttagatg gtgtagatat attatttttg
    19801 atatattcat ctgaagataa acatattaga gatattataa aagctgtttc atatatgtca
    19861 aacgaaagaa gagttctatc tataggaatg gattgttcag agaaggaaaa taaagaagat
    19921 ttagagcttg ggagagagtt taaaatcaat aatgatagta tatttaaatt tgttgattta
    19981 atgaatatca tggtagagtc tatttctgat tcttgcatga taaatataga tattactgat
    20041 ttaaaagaag ctattgtagg agataaagga attaagtatt cttttgaaga gtttgaagat
    20101 acaaagtcat atagcgagat agctgacatt ttatttgaca ggatggaata tattggtgag
    20161 gaatttataa gtaagaaagg aattgtattt gttgaaggaa gtccagaatt ttctataatg
    20221 gaattgaatg atttaataag taatattcag agcaaggtag aagaaagcta tgaagtaata
    20281 ttttctttat atataaagga aaatttaaat ggaaatatta gagtaggctt attgtataat
    20341 tagtatattt tagatattaa ggagagtgta tatgtctgaa aatataagag gtacagagta
    20401 ttggttaatg gaagaatttt tttatcagga aacagacaca ccacagattt ttagagaaga
    20461 aagcataatt gcacttggat ttgatataaa aataaattta aaaaaagtat tcagtatggg
    20521 gaaaaaatca gaaaagtata ttaatgagaa ttgtaatgat aaagatttta taaaaaagtt
    20581 tatgtcgatt aaaaaaggag attatttttt atataaagca tatcaaaata tagaagaaaa
    20641 agttaaatgt aatgtgtcta ttggcatagt tgaagaagat tttgaaaatg gatatgaatt
    20701 aagtcatatt ttagggcata cactaccagt aaaatggatg cataattttg atacagtttc
    20761 aaattgtaaa gtatatacaa gtgaatttta taaattaaat aataatgata ttagttcata
    20821 tttagaaatt attaagtcac aagataagaa attattgaga aaaagaatag aagaaagtga
    20881 aaataataaa tggtatttaa aaaactatta ttataaaaat gtagataagt gtgtagatga
    20941 tgaagaatta tttattcagc ccattaatct agaacaatta gaaagcctaa aaaatataaa
    21001 gacagatgat tatattttat tgtataatag cgagaaagta atttgtgaaa atgaccatac
    21061 aatgaagagt ctatttggcg tatgtcaagt tatggaaggg ttcgataaag agaaagtaag
    21121 tgctttggag ggtggatatt tttttcctgt tagatggata gagtatgaaa aggagaatat
    21181 agacggaaat gcatgggtta tatttgaaaa taatagtata gttgaaaagt atgtaaaaag
    21241 atttactaat aaaatgccat taaatactat actttatgga ccaccaggaa ctggtaaaac
    21301 atacaataca aaacagatgg tttacaatct agtaagtgaa accagtctaa gaaacagata
    21361 ctatacaggt tacttagatt ataaaaataa tgtagaattt tgtacatttc atcaatcata
    21421 tggatatgag gaatttatag agggccttaa gtctgatggt gaaggcaatt ttaagccaga
    21481 agatgggata ttaaaggata tatctataga agcatgttat aatgcattga aatttgaaaa
    21541 aaagatgagc tttgagttgg aaaaagatag cttgacttta gaaactataa aagacaggaa
    21601 aaagaaatta gtattagaaa atatagaata tagtgagagt tttaattttg aagaatcaga
    21661 aaactatgtt ttaataatag atgaaattaa tagagggaat gtatctaaaa tatttggtga
    21721 gctaataaca ttattggaag atgataaaag acttactaaa tcaaatcaaa ctattgtcaa
    21781 actaccatat tcaaaggaaa aattttctct acctccaaat ttgtatatag ttggaactat
    21841 gaacacatca gataaatcaa tagcactttt agatatagct ttaagaagaa ggtttaattt
    21901 tgaagaaatt atgccagatt atgatttatt gtctattgta gatggtatag atattagaaa
    21961 aatgctatat acaatcaata aaaggataga gtttttatat gatagagacc atgttattgg
    22021 acaagcttat ctacttgctg tagaaaatat taatgatata gtagtagtat ttagaagtaa
    22081 aataatacca cttcttcaag agtattttta ttatgattca gaaaaagtag gtttgatttt
    22141 aggaggaatt ggaagaaatg aaaaagataa atacattgtt tataaagaaa aacttatagc
    22201 ttctgagtta tttaaaaata ataatgcctc aataatggag agtaaagtta actattgtat
    22261 aaaacaaaac atatctagtg aggagctaaa aaatatatat gagtaaaaat atatatgtta
    22321 aggaaaccta tgaatggata aggataggta atggtgaaaa tgagcttaca gaaattgaat
    22381 atgaaaaatt gcttaagtat ctagagaaca ataatgatgt attaaaaagt aatataatag
    22441 atattaaata caaaaaactt agatttataa actatgtagg catcatatgt tttgaaaatg
    22501 taatacttga aatacttcct aaattgtcat taagtgataa tcttgtaaaa gatagagaga
    22561 tactattaca gatgctttct atatgtaata aaattccaat tacaatgaat gaaaaaataa
    22621 gattgagttt gaaaaattat aatctcttaa atttttttgt tatgtatttc attgagagta
    22681 tgcaaacaca gatgaaaaaa ggtatttatt ttgaatatat aaataaaata gagaacttaa
    22741 atgttatgag gggtaaaata ttattaagta catatgcaaa agaaaaaggc atttctccaa
    22801 tgaaaataag atgtgaatat gatgagtata gtgaaaataa tttcttgaat caagttctta
    22861 aaaaagcatg tatatctatt ttatgtagaa taaatgacaa ttcaattcaa ggcaagatta
    22921 aaaaaatttt aagctatttt caaaatgtag atttaattta tatagataga aaaaaactgt
    22981 tggattataa attttataaa aacaatga
//
LOCUS       DAYAPA010000059        22936 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.059, whole genome shotgun sequence.
ACCESSION   DAYAPA010000059 DAYAPA010000000
VERSION     DAYAPA010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22936
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.059"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            <1..321
                     /locus_tag="ACGI59_002092"
     CDS             <1..321
                     /locus_tag="ACGI59_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HFG9739878.1"
                     /translation="VEKADCYTGNTIESERFFPGWLYDEEDEFVQAAYKGLKEAGIDS
                     EITQYSFCTNGSHYAGEAGIRTIGFGPSKENLAHTIDEYIELDQLFKGAEGYYGILKS
                     VYKK"
     gene            337..1920
                     /locus_tag="ACGI59_002093"
     CDS             337..1920
                     /locus_tag="ACGI59_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFG9739879.1"
                     /translation="MRILIKHGLIVDGNKTPAYEGDILIENEKILKISQDLNEEADKV
                     IDAKGRVICPGFIDTHSHSDLVILVNPYNEVKIRQGITTEVLGQDGISMAPLPQEHIS
                     SWRKNLAGLDGESDEIDWKYETTENYLKMMDYNGVGLNETYLVPHGNVRMEAMGLEDR
                     PATKEEIQKMCEITERELKAGAIGLSTGLIYIPCAYSLTEEIIEMCKVVAKYDGVFVV
                     HQRSEADTILTSMEEIIEIGKQSGVKVHFSHFKVCGKANWKYIPQVIELLEKAEKEGI
                     RVSFDQYPYAAGSTMLGVVLPPWAHSGGTDKLIERLSDENERAKMKKDIANGIEGWDN
                     FIEFAGIDQIFVTSVKTEKNKDTIGKNLLEIGKMRGKDPLDATFDLLKEEENAVGMVD
                     FYGLEEHIIGFMKRDEQNVCTDGLLAGKPHPRAYGSFPKILGRYVRELNVLTIEEAVY
                     KMTKKAATSFSIKDRGELKEGYFADIVIFDKDTVSGCDDFINSMQYPTGIDYVIINGN
                     CVVEEGKYNKIKAGKVLKN"
     gene            1948..3276
                     /gene="ssnA"
                     /locus_tag="ACGI59_002094"
     CDS             1948..3276
                     /gene="ssnA"
                     /locus_tag="ACGI59_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HFG9739880.1"
                     /translation="MILVGNGRLISQDSLNPYMEDGCVVINDNLIEDIGTTEEMKAKY
                     PVHEFIDAGKKIIMPGLINSHMHIYSSFARGMAVPGKPSENFLEILNNLWWRLDKQLT
                     LEDTKYSAYATYIECIKNGVTTVYDHHASPNAIEGSLFTISDVAKDLGIRTCLCYEVS
                     DRDGGNTIQQGINENVNFIKYAQKDDTDMIKGMFGLHAAFTLSDDTLNKCASEMAGLD
                     TGYHVHVAEGLADLQYNVDKYGKRVLERFNDFGVLKEKTIAVHCIYISAEEISLLNKT
                     NTFVVHNPESNMGNAVGCSPAIELLKRGVTVGLGTDGYTSDMFESLKVANIIHKHHLK
                     DPRVGFEESQTMLFDNNRKIAKAYYKNDLGVLKKGAYADVIVVDYIPHTPLTENTIGG
                     HTIFGMSGRSVDTTIINGKVLMKDRKLINIDEESILAKSRELSQNLWNRI"
     gene            3475..4827
                     /locus_tag="ACGI59_002095"
     CDS             3475..4827
                     /locus_tag="ACGI59_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HFG9739881.1"
                     /translation="MSRDKSPKIAPVDERLPVSKSWVFSLQHVLAMCAGAVAVPMMIG
                     SAARLDHASIVFLINACLFMAGIGTLIQSVGIGNIVGAKIPVIEGTSFAAVAGMTAIA
                     SSYSDSQVAIQTVFGSVIVAGLFCILLAPVFEKLIRFFPSVVTGTVVMVIGISLFPVG
                     IKWITDNKLEAVEPNSLALALAVLAITLLSFKFLKGIWNSTAILVGIVLGTLLSMAVG
                     MADFSAVNGVGWLNLNVPLKFGMPRFEPSAILSLCLIMLVLMTESVGNMIAIHEMVDK
                     EVTGKNIKRGLFGDGISTALSGIFNSFPITPFGQNTGIVGLTGIKSRFIAVYAGVILL
                     VLGFLPKFAAIIGAIPKPVLGGVGFAMFGMVMVGGVKTLSKVKFDGTKNGVIVAVSVG
                     LSMIPLTNPDFYHNFPTWVQTIFHSGITTGSLSAVLLNIFFNELGSKKEKNNKVEEAK
                     "
     gene            5019..6128
                     /locus_tag="ACGI59_002096"
     CDS             5019..6128
                     /locus_tag="ACGI59_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFG9739882.1"
                     /translation="MANLKVNLYGMEFLNPIMTAAGPGAKDGALCQEAAKGGAGAICT
                     KTISVLPADVPRPCMAKTNSGFLNTELWSELPKEQWIEEEYKKVKETGLPVIVSMGYS
                     AEQIAEVAPLVKPYADAVELSTHYVGTDVAPIVNALKAAKAVLDVPVFMKMSPHTDIQ
                     TIAKAVEEAGADGLVMINSFGPCMAIDVNTGYPIMGSKAGYGWLSGAPIRPLAIRCIY
                     EASQVVNIPIIGVGGITCGKDVAEMMMAGASAVQVCTEAILKGPSIYGKIAKELNEFL
                     DENGYADVNEIKGLTHKKMAARGFRTHSVAPVVDDNKCIKCKQCVTSCVYEALEVTDK
                     LNIDADKCFGCGLCVTRCPKGALSICYNQVEAMNE"
     gene            6411..7802
                     /gene="hydA"
                     /locus_tag="ACGI59_002097"
     CDS             6411..7802
                     /gene="hydA"
                     /locus_tag="ACGI59_002097"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HFG9739883.1"
                     /translation="MGIVLKGGKVVTSEKTYCADVRIEDEKIVDIGLDISKENDEIVD
                     VSDSYVIPGGIDTHTHFDLDTGVTKTADNFETGTKAAIVGGTTTILDFATQAKGCSLK
                     DGLEEWHEKAKDKSYSDYGFHMAITDWNDSVCNEMEDMVKEGVSSFKLYMAYKGSLQV
                     DDGVIFEALRKAEEIGGIIGFHCENGDIICELVDKAKSENNLSPKYHQLTRPAIMEAE
                     ATSRLMKIAEVANSKVYVVHLSCKESLEEVLKAKQRGVDVIVETCPQYLLLNDELYDL
                     DGFESAKYVMSPPLRNKENNEILWNAILDNQIDTIGTDHCSFNYKGQKELGINDFSKI
                     PNGSPGVEHRMSLLYTYGVCKNKISINKMVEVTSTNAAKLFGMYPNKGTIEIGSDADI
                     LVLNPNKKSKIEAKKQLQNVDYTPYEGYEVDCQIEHVYLRGKEVVRDGELICNTPSGR
                     YINRRLTKGAREW"
     gene            7796..10357
                     /gene="xdh"
                     /locus_tag="ACGI59_002098"
     CDS             7796..10357
                     /gene="xdh"
                     /locus_tag="ACGI59_002098"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004854 - xanthine dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0046415 - urate metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent xanthine dehydrogenase"
                     /protein_id="HFG9739884.1"
                     /translation="MVEFILNGEKVVAKEGKKLLDFLREDMNITSVKNGCSEGACGTC
                     MVIIDGKATKACVFKTDKLAGKNITTIEGLSERERDVFAYSFASQGAVQCGFCIPGMV
                     ISSKALIDVNNNPTEDDIKKALKGNICRCTGYVKIIKAVDLAAKIFRENIDVPKVNCK
                     GLVGENLPRVDAVGKALGYSEYVDDMRIEGMIYGSAVRAKYPRALVKKIDIEEAKAYP
                     GVVAVLTAKDIPGTIKVGHLKKDWDTLIPEGEITRYLGDGIVLVAAETREALEEAKKL
                     VKIDYEELTPLSTTADALREDAPKIHETGNILVKEHLVRGNAKEKIENSKYVVTKRYT
                     TPATEHAFLEPECAVAGPYEDDGVIIYSTDQGVFATQHECADMLGLPYEKVRVINKMV
                     GGGFGGKEDMSVQHHAALLAYHTKRFVKVQLTRKESIIVHPKRHATEMEFTTACDENG
                     YLTGMKATIISDTGAYASLGGPVLQRLCTHAAGPYNYQDIDITGTAVYTNNPPGGAFR
                     GFGVTQSCFATECNLNLLAEMVGISPWEIRYKNAIRPGQVLPNGQFADEGTAMAETLE
                     AVKEEYEKHPRAGIACAMKNSGVGVGIPDVGRCRLVIKDGKVHIRTSASCIGQGLGTV
                     MTQVVCETANLLPENIVHEVPDTHLTPDSGNTTASRQTVFTGEATKVASNKLKEALDS
                     GKTLLDLEGEEFYGEYESITDKMGCDKENPVSHVAYGYATQVVILDENGKLEKVVAAH
                     DVGRAINPISLEGQIEGGVVMGLGYALTEDYPVVNSIPTAKFGTLGLFKSTDIPEIQA
                     LIVEKNKDLLAYGAKGVGEICTVPTAPAVQNAYYVFDKEFRTELPLQNTPYSKKK"
     gene            10509..11855
                     /gene="rpoN"
                     /locus_tag="ACGI59_002099"
     CDS             10509..11855
                     /gene="rpoN"
                     /locus_tag="ACGI59_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFG9739885.1"
                     /translation="MNFNNSLELTQSQKLIMTTQLKQSLSILNMSKLELEEEIKREAE
                     NNPLVEVEKSGEINWEEYIKDMDRSRRLDRTEISYNPDNEVNLENLVKYSSNLYEDLK
                     FQISLYKLTDKELEVCEYIIDSLDEDGYLRTEEKVIVDTLKIDEELFEKCLISVQQLE
                     PSGVGARSLSECLMIQMASLGIYNEILEEIVTKDLNLIANNKYKEISKKYNMSLQKCV
                     ELINIIKTLDPKPGRTCSVEKSVYIQPDVTVEKIDDEFIVYLNEKDSYQIRINSYYKD
                     ILKNSQSDESAKEFIKERLNSATGLMKNIESRKTTVLKIAEEIVKAQDEFLRKGTKYI
                     KPLKMKDIAEKLEFHESTISRGVNGKYMLTPFGVYEFRYFFSSAIETENNEMASSTSI
                     KKIIKETIKDENKKKPLSDDHISKLLKEKGINVARRTVAKYREELGIPSSSKRKEY"
     gene            12637..13659
                     /locus_tag="ACGI59_002100"
     CDS             12637..13659
                     /locus_tag="ACGI59_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HFG9739886.1"
                     /translation="MKNLLEIQKKLIPQAIELMERRYSILRQISLSEPIGRRTLSNVL
                     EISERIIRSETEFLKEQGLIDVAVSGMTITKEGSDLLDKLKDIMSDIMGLSKLQDRVR
                     EKLGIKRVILVPGSCDENGSLLKDVTRYGCEYFLGILKDGDIVSITGGSTMLEFSNVI
                     KTDKRYPNSTIVPARGSMGTDVEMQSNNIVASISKKLHSNCKLLHIPDELDESAMKTL
                     SQVPEIKTTLEYIKRTDILVFSIGRADVMAKRRKLPEDKVDEILSKNAVGEAFGYYFN
                     KDGEIVYKLNTVGIDLETVKQVKETIAIFAGTEKVEALLAISNMHKNMVLVTDEESAY
                     KILSLL"
     gene            13719..14726
                     /gene="gap"
                     /locus_tag="ACGI59_002101"
     CDS             13719..14726
                     /gene="gap"
                     /locus_tag="ACGI59_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFG9739887.1"
                     /translation="MVKVAINGFGRIGRLALRKMMEQQDKFEVVAINDLTDAKMLAHL
                     FKYDTAQGRFNGEIEVKEGAFVVNGKEIKVTAERNPADLPWAELGVDIVLECTGFFTS
                     KDKAEAHIQAGAKKVVISAPATGDLKTIVFNTNSDILDGSETVISGASCTTNCLAPMA
                     KVLNDKYGIEKGLMTTIHAYTNDQNTLDGPHPKGDLRRARAAAGNIVPNTTGAAKAIG
                     LVIPSLKGKLDGAAQRVPVVTGSITELVCTLGKNVTVEEINAAMKEASNESFGYTEEM
                     LVSSDIIGISYGSLFDATQTKVMEVDGKQLVKVVSWYDNEMSYTSQLIRTLGYFAQLA
                     K"
     gene            14948..16150
                     /gene="pgk"
                     /locus_tag="ACGI59_002102"
     CDS             14948..16150
                     /gene="pgk"
                     /locus_tag="ACGI59_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFG9739888.1"
                     /translation="MSMLNKKTIEDIDVCGKKVLVRCDFNVPLQDGVITDENRLNGAL
                     PTIQYLISKGAKVILCSHLGKPKGEAKPELSLAPVAKRLSEMLGKEVVFAADDNVVGE
                     NAKKATEKMENGDVVLLENTRYRKEETKNEENFSKELASLAEIFVNDAFGTAHRAHCS
                     TVGAGEFLQERVCGYLIQKELKFLGEAVANPVRPFTAILGGAKVSDKLAVINELLEKV
                     DNLIIGGGMAYTFLKAQGYEVGTSLLEIDKVEYAKEMMEKAKNKGVNLLLPVDVVMAD
                     HFAPDATPIVTEDANVKEDYMGLDMGPKTIANFVKTIKESKTVVWNGPMGVFEFENFA
                     NGTLSVARAMAELTDATTVIGGGDSAAAVNQLGFGDKMTHVSTGGGASLEFLEGKELP
                     GIAALDNK"
     gene            16176..16919
                     /gene="tpiA"
                     /locus_tag="ACGI59_002103"
     CDS             16176..16919
                     /gene="tpiA"
                     /locus_tag="ACGI59_002103"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFG9739889.1"
                     /translation="MRKPIIAGNWKMHKTIKEALEFVNEVKDKVNSDKVEAVICAPFT
                     LLKDLKEATKGTNIKIGAQNMHFEEKGAFTGEVSPLMLKEIDMDYVVIGHSERRQYFN
                     ETDETVNKKVLKALEVGIDPILCVGETLEQREAGKTKDVCKVQVEKALENVLKDDLAK
                     VVVAYEPIWAIGTGKTATAEDANDVISYIREVIKGLYGELANEVRIQYGGSVKPSNVA
                     EIMGQSDIDGALVGGASLASNDYLDLVNF"
     gene            16931..18460
                     /gene="gpmI"
                     /locus_tag="ACGI59_002104"
     CDS             16931..18460
                     /gene="gpmI"
                     /locus_tag="ACGI59_002104"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HFG9739890.1"
                     /translation="MKKPVALIIMDGFGYNKDVKGNAIAESKTPNLDRIKKEYPNTLI
                     NASGLDVGLPDGQMGNSEVGHTNIGAGRIVYQDLTRITKSIKDGDFFTNKVLCEAMDN
                     AKENSLHVMGLLSDGGVHSHIDHLKAIIKMAKDKGVQKVYVHAFTDGRDTDPQSALEY
                     AKEVQASMDEIGVGEFATVSGRYYAMDRDKRWERVELAYNAMVRGIGEKANSIEEAIQ
                     NSYDDGKNDEFIMPTVIMKDDKPVGSIKENDSIIFFNFRPDRARQITRALVCEEFDGF
                     KREDIKNFFVCLTEYDITIENVHIAFGPQSLANTLGEYLAKNGKTQLRAAETEKYAHV
                     TFFFNGGVEEPNKGEERLLIPSPKVATYDLKPEMSAYELTDKALDKLGEDKFDFIVLN
                     FANPDMVGHTGSIEAAIKAVETVDTCVGRLIDKIVELGGSAIITADHGNAEYMLDPET
                     GKTVTAHSINPVPFIVVGQEYESAKLLDGGRLSDIAPTILDMMKLEKPEEMTGHSLIS
                     K"
     gene            19048..20340
                     /gene="eno"
                     /locus_tag="ACGI59_002105"
     CDS             19048..20340
                     /gene="eno"
                     /locus_tag="ACGI59_002105"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFG9739891.1"
                     /translation="MSVIELVYAREVLDSRGNPTVEVEVVLEDGAMGRAIVPSGASTG
                     AFEAVELRDGDKGRYLGKGVETAVANVNEIIAPEIEGMDAFDQPAIDAIMIELDGTPN
                     KGKLGANAILGVSMAVARAAADEIGLPLFQYLGGVNAKQLPVPMMNILNGGEHADNNV
                     DVQEFMILPVGACCFKEGLRMGAEVFHSLKKVLGEKGLACGVGDEGGFAPNLGSNREA
                     LELIVEAITKAGYKPGEDVMLGLDVAATEMYNKETKKYVLAGEGKELTAAEMVALYED
                     WSNNFPIITIEDGLDEEDWDGWKLLTEKLGNKLQLVGDDLFVTNTERLEKGIENGVAN
                     SILVKVNQIGTITETLDAIEMAKRAGYTAVISHRSGETEDSTIADLAVAVNAGQIKTG
                     APSRTDRVAKYNQLLRIEEMVGEQARYCGLKSFYNLKK"
     gene            20933..21157
                     /gene="secG"
                     /locus_tag="ACGI59_002106"
     CDS             20933..21157
                     /gene="secG"
                     /locus_tag="ACGI59_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFG9739892.1"
                     /translation="MSNILMGVQVVLGILLVISIMPQDSKNALPSEFGGEGSQAYFKP
                     KGKQAFLARVTKITAVLFFINALALLIVKK"
     gene            complement(21541..22479)
                     /locus_tag="ACGI59_002107"
     CDS             complement(21541..22479)
                     /locus_tag="ACGI59_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HFG9739893.1"
                     /translation="MENLILSINVVLPLFLTMSLGYVLKKLGLFDTHTLNKMNNVCFK
                     SFLPLLLFFNIYNTDLDGSLNFKLMAVAVIIIFSIFLILVFIIPKIEKDNKTRGALIQ
                     GVFRSNFVIFGIPVTISLFGDKSIGVTSLLIAVIVPMFNVLSVVILEIFRGGKVNIKH
                     MIKSVLTNPLIIASFIGLTMLILNINIPTFLHKTIGDISKIATPLSLIILGGSFSFSA
                     IKGHIKPIILGVCGKLILVPAIFLPISIFLGFRNVELASLMIMLSAPTAVSSFTMAQQ
                     MDSDSELAGQIVVFTSGFSIITVFLIIFILKQNNLI"
     gene            complement(22467..22865)
                     /locus_tag="ACGI59_002108"
     CDS             complement(22467..22865)
                     /locus_tag="ACGI59_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFG9739894.1"
                     /translation="MIKKIEHVVITNNNIENYINFYKKFGFSSKEMSKNYKLNARNVK
                     INLNIKGYKFLPYVKNSKASRDNLCFKINTNIDEPKKYLEQNEIQIKRGIVECTGVFG
                     NMKSIYLKKTNGNLIELSNHRITESKPWKI"
BASE COUNT         9119 a         2624 c         4433 g         6760 t
ORIGIN      
        1 gttgaaaaag ctgattgcta tacaggaaat actatagaaa gtgaaagatt cttcccaggt
       61 tggctatatg atgaagaaga tgaatttgtt caagcagcgt ataagggatt aaaagaagct
      121 ggaatagatt cagaaataac tcaatactca ttctgtacga atggaagtca ctacgcaggt
      181 gaggctggaa taagaactat aggatttggt ccttctaagg aaaatcttgc tcatacaata
      241 gatgagtata tagaattaga ccaattattc aaaggtgcag aaggatatta tggtatatta
      301 aaatcagttt ataaaaaata attaggagat ggttttgtga gaattttaat aaaacatggg
      361 cttatagtag atggaaataa aacaccagca tatgaaggag atattttaat agaaaacgag
      421 aaaatattaa aaatatcaca agatttaaac gaagaagctg acaaagttat agatgcaaaa
      481 ggtagagtta tatgtccagg tttcatagat actcatagcc attcagacct agttatttta
      541 gttaatccat ataatgaggt aaagattaga cagggaatta caacagaagt gttgggccaa
      601 gatggtatat ctatggcccc tctacctcaa gagcatatat cttcttggag aaaaaattta
      661 gcaggtctag atggtgaaag tgatgaaatt gattggaaat atgaaacaac agagaattat
      721 ttaaaaatga tggattacaa tggggtaggt ttaaatgaaa cttatttagt acctcatgga
      781 aacgttagaa tggaagctat gggacttgaa gatagacctg ctactaaaga agaaattcaa
      841 aaaatgtgtg aaatcacaga gagagaatta aaagcaggag ctataggatt gtctactgga
      901 cttatatata taccttgtgc atactcattg actgaagaaa ttatagagat gtgtaaggta
      961 gtagcaaaat atgatggagt atttgtagtt caccaaagaa gtgaagcaga tacaatttta
     1021 acttcaatgg aagaaatcat agaaattgga aaacaatcag gagtaaaagt tcatttttca
     1081 cattttaagg tttgtggtaa agcaaactgg aagtatatac cacaagttat agagctttta
     1141 gaaaaagcag aaaaggaagg aataagagta tcttttgacc aatatccata tgcagcagga
     1201 agtacaatgc ttggagtagt tttacctcct tgggcacatt ctggaggaac tgataagtta
     1261 atagaaagat taagtgatga aaacgaaaga gcaaaaatga aaaaagatat agcaaatgga
     1321 atagaaggct gggataattt tatagaattt gctggaatag accaaatatt tgtaacaagc
     1381 gtaaaaacag aaaagaataa agatacaatt ggtaagaact tattagaaat tggaaaaatg
     1441 agaggaaaag acccattaga tgcaacgttt gatttattaa aagaagaaga aaatgcagtt
     1501 gggatggtag atttctatgg attagaagaa catataatag gattcatgaa aagagacgag
     1561 caaaatgtgt gtacagatgg tcttttagca ggtaaaccac atccaagagc ttatggttct
     1621 ttccctaaaa tactaggaag atatgttaga gagttgaatg tgttaacaat cgaagaagct
     1681 gtttataaaa tgactaaaaa ggcagctaca agctttagta taaaagatag aggcgaatta
     1741 aaagaaggat attttgcaga tatagttata tttgacaagg atacagttag tggttgtgat
     1801 gattttatca actctatgca gtaccctaca ggtatagatt acgtaattat aaatggaaat
     1861 tgtgttgttg aagaaggaaa atacaataaa attaaagcag gaaaagtact taaaaattaa
     1921 atcttagctt taaggaggaa taaatttatg attttagtgg ggaatgggag gctaataagc
     1981 caagattctt taaatcccta tatggaagat ggatgtgtag taataaatga taatctaatt
     2041 gaagatatag gtactacaga agaaatgaaa gctaaatatc cagtacatga atttatagat
     2101 gcaggcaaga aaataatcat gccgggtcta ataaattcac atatgcatat atacagctca
     2161 tttgctagag gaatggcagt tccaggaaaa ccttctgaga actttttaga gatactaaac
     2221 aatttatggt ggagacttga caaacagctt acactggaag atacaaaata cagtgcatat
     2281 gcaacgtata tagaatgtat aaaaaatggt gtaacaacag tttatgacca tcatgcaagt
     2341 ccaaatgcaa ttgaaggcag tttgtttaca atatcagatg ttgcaaagga tttgggaatt
     2401 agaacgtgtc tttgttatga agtgtctgat agagatggtg gaaatacaat ccaacaaggt
     2461 atcaatgaaa acgtaaactt tataaaatac gctcaaaaag atgatacaga catgataaaa
     2521 ggaatgtttg ggcttcatgc tgcatttaca ctatctgatg atacattaaa taaatgtgct
     2581 tcagagatgg ctggtcttga tacaggttac catgtacatg ttgctgaagg actagcagac
     2641 ttacaatata atgtagataa atatggaaaa agggttttag aaagattcaa tgattttgga
     2701 gtactaaaag aaaaaacaat agcagttcat tgtatatata taagtgcaga agaaatcagt
     2761 cttttaaata aaacaaatac atttgtagtt cataatccag aatctaatat gggaaatgca
     2821 gttggatgtt ctcctgcaat agagttatta aaaaggggag ttacagtagg attaggaact
     2881 gatggatata ctagcgatat gtttgaatca ttaaaagttg ccaatataat tcacaaacat
     2941 cacttaaaag acccaagagt tggatttgaa gaatcacaaa caatgttatt tgataacaat
     3001 agaaaaatag ctaaagctta ttataaaaat gacctaggag tacttaaaaa aggtgcttat
     3061 gcagatgtta tagtagttga ttatatacca catactccat tgacagaaaa tacaataggg
     3121 ggacacacta tttttggaat gagtggaaga agcgtagata caactattat aaatggaaag
     3181 gtacttatga aagatagaaa gttaataaac atagatgaag aaagtattct agcaaaaagt
     3241 agagaattaa gtcaaaatct atggaataga atttaaataa ttgattatgc aaaattctat
     3301 gaagcaggaa ccctgtgact ttagtcatgg gaggttcaga ttaatataca agtaagaaac
     3361 aagaatttag atgttaaaaa cagagcttaa acaaaaatag gaatttaaat gaagtatata
     3421 aataaaaaat atacggaata gaatattaaa ttaaatatag aagggagacc caaaatgtct
     3481 cgagataaaa gccctaaaat agctccagta gatgaaaggt tacctgtatc caaatcttgg
     3541 gtattttcac tacagcacgt tttagcaatg tgtgcaggag cagtagcagt tcctatgatg
     3601 ataggaagtg ctgcacgact agatcatgct agtattgttt ttttaataaa tgcttgcttg
     3661 tttatggcag gtataggaac actcatacaa agtgttggta taggaaatat tgtaggtgct
     3721 aaaataccag taatagaagg aactagtttt gccgctgtgg caggtatgac agcaatagcc
     3781 tcatcatata gtgattcaca agttgcaata cagacagtat ttggctctgt aatagtagct
     3841 ggattgtttt gtatattact tgctccagta tttgaaaaat taataagatt tttccctagt
     3901 gtagttactg gaacagttgt tatggtaata ggtatttcac tatttccagt tgggataaaa
     3961 tggataactg acaacaaatt ggaagcggtt gaaccaaact cattagcatt agctttagca
     4021 gtattggcaa taactttact ttcatttaaa ttcttaaaag gaatttggaa tagcacagct
     4081 atattggtag gtatagtact tggtacacta ctttccatgg cagttggaat ggcagatttt
     4141 agtgcagtaa atggagttgg ttggcttaac ttaaatgtac ctttaaaatt tggaatgcct
     4201 agatttgaac catctgcaat actatcattg tgtttgatta tgttggtact tatgacggaa
     4261 tcagttggta atatgatagc tattcatgaa atggtagaca aagaagtaac aggtaaaaat
     4321 attaaaagag gtctatttgg agatggaata tctacagcat tatctggaat attcaattca
     4381 tttccaataa ctccatttgg tcaaaataca ggaatagtag gtcttacagg tataaaatca
     4441 agatttatag cagtttacgc aggggttata ttgctagtac ttggattctt accaaaattt
     4501 gcagcaataa taggagcaat acctaagcct gttcttggag gcgtaggatt tgcaatgttt
     4561 ggaatggtaa tggttggagg agtaaaaact ttaagcaaag tgaaatttga tggaactaaa
     4621 aatggggtta tagtagcagt tagtgttggt ttgtctatga taccactaac aaatcctgat
     4681 ttctatcata atttcccaac ttgggtacaa acaatattcc atagtggaat aacaacaggt
     4741 agtttatcag cagtgctttt aaacatattc ttcaatgaac taggttctaa aaaagaaaaa
     4801 aataataaag ttgaagaagc aaaataaaaa taaaacaagt aaaaatctat attgacttaa
     4861 agtcattaag tattaataaa aataattcag agatattaat aattcaacaa aactatctta
     4921 tattttaaat aatataaaaa agtaaataaa taacaaatac aaataaaaga atacaatcta
     4981 aatattaaaa atatttaatt taagggagag ataataatat ggctaattta aaagttaatt
     5041 tatatggaat ggaattttta aatccaatta tgacagcagc aggacctgga gcaaaagatg
     5101 gtgctctctg tcaagaggca gcaaaaggtg gagcaggagc tatatgtaca aaaactatat
     5161 ctgtattacc agctgatgta ccaagaccat gtatggcaaa aactaatagt ggttttttaa
     5221 acacagaatt atggtcagag cttccaaaag agcaatggat agaggaagaa tacaaaaagg
     5281 taaaagaaac agggcttcca gtaatagtaa gtatgggata ttcagcagag caaatagctg
     5341 aggttgctcc tttagtaaag ccttatgcag atgcagttga attatctact cattatgtag
     5401 gtacagacgt tgcaccaata gtaaatgctt taaaagctgc aaaagctgtt ttagatgtac
     5461 cagtatttat gaaaatgagt cctcatactg acattcaaac tattgcaaaa gctgttgaag
     5521 aagcaggtgc agatggactt gtaatgataa actcatttgg accatgtatg gcaatagatg
     5581 taaatacagg ataccctata atgggaagta aagctggata cggatggtta tcaggtgcac
     5641 caataagacc tctagcaata agatgtatat atgaagcttc tcaagttgta aatataccaa
     5701 taatcggtgt tggtggaatc acttgtggaa aagacgtggc tgaaatgatg atggctggag
     5761 caagtgctgt tcaagtatgt acagaagcaa tccttaaagg gccttctatc tatggaaaaa
     5821 ttgctaaaga attaaatgaa tttttagatg agaatggata tgctgatgtt aatgaaataa
     5881 aaggactaac tcataagaaa atggcagcta gagggtttag aactcattca gtagctccag
     5941 tagtggatga taacaagtgt atcaaatgta agcaatgtgt aacaagctgt gtatacgaag
     6001 ctttagaagt tacagataag ttgaacatag atgcagacaa gtgttttgga tgtggattat
     6061 gtgtaactag atgtccaaaa ggagctttaa gcatctgcta caatcaagta gaagctatga
     6121 atgagtaata attatataga attttggtaa ttaacttaga gtaataggta ttaggataat
     6181 aaaatatatc agataaacaa acacacatta tgcattaata atacagtcta aagttaataa
     6241 ttttattata ttgatttaac taaatattag tttagctgaa tattaattta attaggtatt
     6301 ggtttaatct taatatagat ttactgcgta taagtgaact aactagatta ctttaagtta
     6361 attttttcat ataaaataaa ttgatacatt acaagtaagg tggagtctat atgggaattg
     6421 ttttaaaagg tgggaaagta gtaacaagtg aaaaaactta ctgtgcagat gtaagaatag
     6481 aagatgaaaa aatagtcgat ataggtcttg atatatcaaa agaaaatgat gaaatagttg
     6541 atgttagtga ttcttatgtt ataccaggag gtatagatac acacacacac tttgacttag
     6601 atacaggcgt tactaagaca gcagataact ttgagacagg aacaaaagct gctatagtag
     6661 gaggaactac tactatattg gattttgcta cacaagctaa aggatgttct ttgaaagacg
     6721 gattagaaga atggcatgaa aaagctaagg ataaatccta ttcagattat ggctttcata
     6781 tggctataac agattggaat gactcggttt gtaatgaaat ggaggatatg gtgaaagagg
     6841 gagtatcttc atttaaactg tatatggcgt ataaaggaag ccttcaagtt gatgatggtg
     6901 taatctttga agcattgaga aaagcagaag agataggagg gattattggt ttccattgtg
     6961 aaaatgggga cataatatgt gaacttgtgg ataaagcaaa gtcagaaaat aatttatctc
     7021 caaaatatca tcaattaact agaccagcta taatggaggc agaagctaca tcaagactta
     7081 tgaagatagc tgaagttgca aactctaaag tatatgtagt tcacttaagc tgtaaagaat
     7141 ccttagagga ggttttaaaa gctaagcaaa gaggtgtgga tgtaatagtt gaaacttgtc
     7201 ctcaatacct tctattaaat gatgaattat atgatttaga tggatttgaa agtgctaagt
     7261 atgtaatgtc accaccttta agaaataaag aaaataatga aatattatgg aatgctattt
     7321 tagataatca aatagacact ataggaacag accattgttc ttttaattat aagggacaga
     7381 aggaacttgg gataaacgat ttcagtaaga taccaaatgg ttctccaggt gtagaacata
     7441 ggatgagtct tttatacaca tatggagtct gtaaaaataa aatatctatc aacaagatgg
     7501 tagaagtaac atcaactaat gcagcaaagt tgtttggaat gtatcctaat aaaggaacta
     7561 tagaaatagg cagtgatgca gatatattag tcttaaatcc aaacaaaaaa tccaaaatag
     7621 aagcaaaaaa acaattacaa aatgtagatt atacacctta tgaagggtat gaagtggatt
     7681 gtcaaataga acatgtttat ttaagaggaa aagaagtagt tagagatgga gaattaattt
     7741 gtaatactcc aagtgggaga tatataaata ggcgtctaac aaagggggct agagaatggt
     7801 agaatttata ttaaatggag agaaagttgt tgcaaaagaa ggcaaaaaac ttttagactt
     7861 ccttagagaa gatatgaata taacttcagt taaaaatgga tgttcagaag gtgcttgtgg
     7921 aacatgtatg gttataatag atggtaaagc tactaaagca tgtgtattta aaactgataa
     7981 attagctggg aaaaacataa caactataga gggattatca gagagagaaa gagatgtttt
     8041 tgcatattct tttgctagtc aaggagctgt acaatgtgga ttttgtattc caggtatggt
     8101 aattagttca aaagcactta tagacgttaa taataatcct actgaagatg atataaagaa
     8161 agctttaaaa ggtaatatat gtagatgtac agggtatgta aaaataatta aagctgtcga
     8221 tttagcggca aagattttta gagaaaatat agatgtgcca aaagtaaact gtaagggtct
     8281 agtaggtgaa aatcttccta gagttgatgc agttggcaaa gcacttggat attctgaata
     8341 tgtagatgat atgagaattg agggtatgat ttatggttca gcagttagag ctaagtatcc
     8401 tagagcttta gttaaaaaaa tagatataga agaagcaaaa gcttatccag gagttgttgc
     8461 agttctaaca gctaaagata ttccaggaac tataaaggta gggcacttaa agaaggattg
     8521 ggatacttta ataccagaag gtgaaataac tagatactta ggagatggaa tagttttagt
     8581 agcagcagaa actagagaag cgttagaaga ggccaaaaag ctagttaaga tagattatga
     8641 ggaattaaca ccattaagta ctacagcaga tgcacttaga gaagatgctc caaagataca
     8701 tgagacaggg aatatacttg ttaaggaaca tttagtaaga ggaaacgcta aagaaaagat
     8761 agaaaattca aaatatgttg taactaaaag atatactaca cctgctacag aacacgcatt
     8821 tttagagcct gaatgtgcag tagctggtcc ttatgaagat gatggagtaa taatctattc
     8881 gacagaccaa ggggtgtttg ccactcaaca tgaatgtgca gatatgttag ggttaccata
     8941 tgagaaggta agagttataa acaaaatggt aggaggaggg tttggtggaa aagaagacat
     9001 gagtgttcaa caccatgcag ccttacttgc ataccataca aagagatttg taaaagtaca
     9061 gttaacaaga aaagaaagta ttattgttca tccaaaacga catgccacag aaatggaatt
     9121 tacgacagca tgtgatgaaa atggatactt aacgggtatg aaggcaacga taatatctga
     9181 cacaggtgca tatgcctctc ttggaggacc agtgctacaa agactgtgta ctcatgcagc
     9241 aggaccatac aattatcaag atattgatat aacaggaaca gcagtatata ctaataaccc
     9301 tcctggagga gcatttagag gatttggagt tacacagagt tgttttgcaa ctgaatgtaa
     9361 tctaaattta cttgctgaaa tggttggaat atctccttgg gagataagat ataaaaatgc
     9421 aataagacct gggcaagttt taccaaatgg tcaatttgca gatgaaggaa cagcaatggc
     9481 agagacacta gaagctgtaa aagaagagta tgaaaagcat ccacgtgcag gtatagcttg
     9541 tgctatgaaa aatagtgggg ttggagttgg tatacctgat gttggaagat gtagattggt
     9601 gataaaagat ggcaaagttc acataagaac aagtgcttct tgtataggtc aagggcttgg
     9661 tactgttatg actcaagtag tttgtgaaac tgctaaccta cttcctgaaa atatagttca
     9721 tgaagttcca gatactcatt taacaccaga ctctggtaat actacggctt caagacagac
     9781 agtatttacg ggagaagcta ctaaggttgc atcaaataag ttaaaagaag ctttagactc
     9841 tggcaaaact ttacttgatt tagagggaga agagttttat ggagaatatg aaagtattac
     9901 agataaaatg ggttgtgaca aagaaaatcc agtaagtcat gttgcttatg gatatgcaac
     9961 acaagttgta attctggatg aaaatggaaa actagaaaaa gtagtagcag cacatgatgt
    10021 tggaagagct ataaatccaa tcagtttaga aggtcaaata gaaggtggag tagttatggg
    10081 tcttggatat gctctaaccg aagattaccc agtagttaac tctattccta cagctaagtt
    10141 tggtacatta ggtttattta aatctacaga tataccagaa atacaagctt taatagttga
    10201 aaagaacaaa gatttattgg cttatggggc aaaaggtgta ggagaaattt gtacagttcc
    10261 tactgcacca gcagtacaaa atgcctatta tgtatttgat aaagaattta gaactgagtt
    10321 accactacaa aatactccat atagtaaaaa aaagtaacaa aaaaaagaaa tattaaataa
    10381 aaaaatagat gaaattgtaa ataaaaaaaa cccttttgca taaggtatat tttaaatgat
    10441 agaatataca tatacaggag ggtttttctt ttataaaata agtttaataa aattgagagg
    10501 tgagcagaat gaattttaat aatagtctag aattaactca gtctcagaag ctaataatga
    10561 ctacacaact taagcaatca ttatcaatat taaatatgag taagttagag ttagaagaag
    10621 agataaaaag agaagcagag aataacccat tagttgaagt cgagaaaagt ggggagataa
    10681 attgggaaga gtatataaaa gacatggata ggtcaagacg tttagataga acagagataa
    10741 gctataatcc tgataatgaa gttaatctag aaaatttagt taaatattcg tcaaatttat
    10801 atgaagattt aaaatttcag ataagcctgt ataaacttac agacaaagaa ttggaagtct
    10861 gtgagtatat aattgatagt ttagatgagg atggatattt aagaactgag gaaaaagtaa
    10921 ttgttgatac cttaaagata gatgaagaac tttttgaaaa atgtttaatt agtgttcagc
    10981 agttggaacc tagtggagta ggggcaagaa gtctttcaga atgtctgatg atacaaatgg
    11041 ctagtttagg aatatataat gaaattttag aggaaatcgt tacaaaagac ttaaatttaa
    11101 ttgcaaataa taaatacaaa gagataagta aaaagtacaa tatgtcatta caaaaatgtg
    11161 ttgaactaat aaatataatt aagacattag accccaaacc aggaagaact tgttcagtag
    11221 agaaaagtgt atacattcaa cctgatgtaa cagtagaaaa aattgatgat gaatttattg
    11281 tatatttaaa tgagaaagat tcttatcaaa taagaatcaa tagttattat aaagacatat
    11341 taaaaaattc acagtctgat gaaagtgcaa aagaatttat aaaagaaaga ctaaattcag
    11401 caacaggact tatgaaaaat atagagagta ggaaaactac agtacttaag attgcagaag
    11461 agatagtaaa agctcaagat gaatttttga gaaaagggac aaaatatata aaacctttga
    11521 aaatgaaaga catagcagaa aaattagaat ttcatgaatc tactataagt agaggtgtaa
    11581 atggtaaata tatgctaaca ccttttggcg tatatgaatt taggtacttt ttcagtagcg
    11641 ccattgagac tgagaataat gaaatggcat ctagtacaag cataaaaaaa attataaagg
    11701 aaacaataaa agacgaaaat aaaaagaaac ctttaagtga tgaccatata tctaagttat
    11761 taaaagaaaa aggaatcaat gttgcaagaa ggacagttgc caaatatcgt gaagaattag
    11821 gaatcccatc ttcaagtaag agaaaagaat actaataagt aaaagacaaa tatcagtatg
    11881 gtaaggggaa aaggctaatt aagagatgaa tgtagattgg gctaaaaatt ttataaagta
    11941 aaataatata atatgaaaaa gtattaaaat gctataaggt attaaaaaac tggggaacat
    12001 taaaacatta agatagcaaa gagcataaaa aactaaagaa gattaaaaca ttaaagaaca
    12061 ttagagcttt gaggaatatt agaactttaa agaatattaa aatttataaa atataaaaat
    12121 actaaaaaga attgaaaaac tggggaacat taaaatattg taaaatgcta aaaaactaga
    12181 aaacataatt aaataagtgc ttataaagaa tgattaaatt aaataagtgt ttgtaaagaa
    12241 taattagata ttagaaaaag aagaatacct agataaataa actattatta aatttaataa
    12301 tcacaaaagg aaaacacgta gggtataaat aaaaatctta actagataaa agtaaactta
    12361 gttgtagata aaaataacta gaaataaaat tatttatagt aataaatatt agaatgaaaa
    12421 tataaaaaaa tatatataaa aagccttata taaaaagaaa aaatactgtg atttgaaaat
    12481 ggtggagaaa tattttataa aataaaaaaa taaaaaagtt tttgaaaaac tattgaaatc
    12541 tatacgcaaa tcctataaaa taaaaatatc ttgtgggact taaataaaca ctagggacaa
    12601 aaaaagtccc gaaaggaaag caataaggag tatgttatga agaacttatt agaaattcaa
    12661 aagaaattga ttccacaggc aattgaactt atggaaagaa gatactctat tttaagacaa
    12721 atatctttaa gtgaaccaat aggtaggaga acattatcaa atgtattaga gattagtgaa
    12781 agaataatca ggtcagaaac tgagttttta aaagaacaag gacttataga cgtagctgta
    12841 tctgggatga ctatcactaa agaaggttct gatttgttag ataaacttaa ggacatcatg
    12901 agtgacatca tgggattgtc taagttacaa gatagagtca gagaaaagct tggaataaaa
    12961 agggtaatat tagttcctgg aagctgtgat gaaaatggaa gtttactaaa agatgtaact
    13021 agatatggat gcgaatattt cttaggcatt ttaaaagatg gagatatcgt ttcaataaca
    13081 ggaggaagca ctatgttgga gttttcaaac gttattaaaa ctgataagag atatccaaac
    13141 tctacaatag ttccagctag aggaagtatg ggaactgatg tagagatgca atctaacaat
    13201 atagttgctt caataagtaa gaaattacat tctaattgca aacttttaca tataccagat
    13261 gagttggatg aaagtgcaat gaaaactctt agccaagtgc cagagattaa gacaactttg
    13321 gagtatatta aaagaacaga cattttagtt tttagtatag gtagagcaga tgtcatggca
    13381 aagagaagaa aacttccaga agacaaggtt gatgagatat tatctaaaaa tgcagttgga
    13441 gaagcatttg gatattattt caacaaagat ggagaaatag tttacaaact gaacactgtt
    13501 ggaattgatt tagaaacagt taaacaagta aaagaaacta ttgcaatctt tgcagggact
    13561 gaaaaagtag aagccttatt agctatatca aatatgcata aaaacatggt tcttgtaaca
    13621 gatgaagaaa gtgcttataa gatattgtca ttattataaa agtcaagacc tagcacagag
    13681 ctagaaaaca tacatttact ttataggagg tagcgaaaat ggttaaagta gctataaatg
    13741 gatttggaag aataggaaga ttagcattaa gaaaaatgat ggagcaacaa gataagttcg
    13801 aagttgttgc aataaacgac ttaacagacg ctaaaatgtt agctcattta tttaaatatg
    13861 atacagctca aggaagattt aacggagaaa tagaagttaa agaaggtgct tttgtagtaa
    13921 acggaaaaga aataaaagta acagcagaaa gaaatcctgc tgacctacca tgggctgaat
    13981 taggagtaga tatagtatta gaatgtacag gattctttac atctaaagat aaagctgaag
    14041 ctcatataca agcaggggct aagaaagttg ttatatctgc accagctact ggagatttaa
    14101 aaacaatagt attcaacaca aacagtgaca tattagatgg ttctgaaaca gttatatcag
    14161 gagcttcttg tactactaac tgtttagctc caatggctaa agtattaaat gataaatacg
    14221 gtatagaaaa aggcttaatg actactatac atgcatacac taatgaccaa aatactttag
    14281 atggtcctca tccaaaaggt gatttaagaa gagctagagc tgctgctgga aacatagttc
    14341 caaacactac aggagctgca aaagcaatag gcttagtaat accttcatta aaaggaaaat
    14401 tagatggggc agcacaaaga gtaccagttg taactggttc aataactgaa ttagtttgta
    14461 cattaggaaa aaatgttaca gttgaagaaa taaatgcagc tatgaaagaa gcttcaaacg
    14521 aatctttcgg atatactgaa gaaatgttag tatcttctga cataatcgga ataagctacg
    14581 gttcattatt tgacgctact caaacaaaag ttatggaagt agatggaaaa caattagtta
    14641 aagttgtttc ttggtatgat aatgaaatgt cttatacttc tcaattaata agaactttag
    14701 gatactttgc acaattagca aaatagtcag ttttgagaaa atagaaaagt agattgacaa
    14761 agtccgagtt tgtagtttaa tactacggac tcaggacttt ttttaagaaa aaatatataa
    14821 aatacaccta aataatgtta catactatat ataaaaatat gataatatga tatatttaat
    14881 agggtaaaag tatgtactat attatacttg gcaataaaaa attactttat taaaaggaga
    14941 gatatttatg tcaatgctta acaaaaaaac tatagaagat attgatgtgt gcggaaaaaa
    15001 agtattagta agatgtgact ttaatgtgcc attacaagat ggtgtaatta ctgatgaaaa
    15061 tagattgaac ggagctttac caactattca atatttaatt tctaaagggg caaaagttat
    15121 attatgctct cacttaggaa agccaaaagg agaagctaaa ccagaattat ctttagcacc
    15181 agtagcaaag agattatctg agatgttagg aaaagaagtt gtatttgcag cagacgataa
    15241 tgttgtagga gaaaatgcaa agaaagcaac tgaaaaaatg gaaaacggag atgtagtatt
    15301 attagaaaat actagatata gaaaagaaga aactaaaaac gaagagaatt tctctaaaga
    15361 actagcatca cttgctgaaa tatttgtaaa tgatgcattt ggaactgctc atagagcaca
    15421 ttgctcaaca gttggtgcag gcgagttttt acaagagaga gtttgtggat acttaattca
    15481 aaaagaatta aaattcttag gagaagctgt tgcaaaccct gtaagaccat ttacagctat
    15541 acttggagga gctaaagttt ctgataaatt agctgttata aacgaattat tagaaaaagt
    15601 tgataatcta ataataggtg gaggaatggc ttatacattc ttaaaagctc agggttatga
    15661 agtaggaact tctttacttg aaatagataa agttgaatat gctaaagaaa tgatggaaaa
    15721 agctaagaat aagggtgtta acttattatt accagtagac gttgttatgg cagaccactt
    15781 tgcaccagat gctactccaa tagtaacaga agatgctaat gtaaaagaag attacatggg
    15841 actagatatg ggacctaaga ctatagcaaa ctttgtaaaa actataaaag aatcaaaaac
    15901 tgtagtatgg aatgggccaa tgggagtatt tgaatttgaa aactttgcaa atggtacatt
    15961 atcagtagct agagctatgg cagagttgac tgatgcaact actgtaattg gcggtggaga
    16021 tagtgcagca gcagttaatc aattaggatt tggtgacaag atgactcacg tatcaacagg
    16081 tggaggagca tcactagaat ttttagaagg taaagaatta ccaggtattg cagcattaga
    16141 taataaataa actaatatat agaggagatt gtaaaatgag aaaacctata attgcaggaa
    16201 actggaaaat gcataaaaca ataaaagaag ctttagaatt tgtaaatgaa gttaaagata
    16261 aagtaaattc tgataaagta gaagctgtta tatgtgcacc atttacttta ttaaaggatt
    16321 taaaagaagc tactaagggt acaaatataa agataggtgc tcaaaatatg cactttgaag
    16381 aaaaaggagc atttacagga gaagtttcac ctcttatgtt aaaagaaata gatatggact
    16441 atgttgtaat aggacattca gaaagaagac aatacttcaa tgaaactgat gaaactgtaa
    16501 acaagaaagt tttaaaagca ttagaagtag gaatagaccc aatattatgt gttggagaaa
    16561 ctttagaaca aagagaagct ggaaaaacta aagatgtttg taaagttcaa gttgaaaaag
    16621 ctttagaaaa tgttcttaaa gatgatttag caaaagtagt tgttgcatat gagccaatct
    16681 gggctattgg aactggtaaa actgcaactg ctgaagatgc taatgacgtt atatcttata
    16741 taagagaagt tataaaagga ttatatggag aattagctaa tgaagttaga atacaatacg
    16801 gtggaagtgt taaaccttca aatgtagccg aaataatggg tcaaagtgat atagatggag
    16861 ctttagtagg tggagctagt ttagctagta atgattacct agaccttgtt aatttctaag
    16921 gagtgaaatg atgaaaaaac cagttgcttt aattattatg gacgggtttg gatacaataa
    16981 ggatgttaag ggaaatgcaa ttgctgagtc caaaactcct aatttagata gaataaaaaa
    17041 agagtatcca aacacattga taaatgctag tggtcttgat gttggtctac ctgatggtca
    17101 aatgggaaat tcagaagttg gacacactaa cataggtgca ggtagaatag tatatcaaga
    17161 tttaactaga ataacaaaat ctataaaaga tggtgacttt tttactaata aagttttatg
    17221 tgaagcaatg gataatgcaa aagaaaactc tcttcatgta atgggacttt tgtctgatgg
    17281 aggagtacat tcacatatag accatcttaa ggctataatc aaaatggcaa aagataaagg
    17341 cgtacaaaag gtgtatgttc acgcatttac tgatggtaga gatactgacc ctcaaagtgc
    17401 attagaatat gccaaagaag tacaagctag tatggatgaa attggagtag gtgaatttgc
    17461 tacagtttct ggtagatact acgctatgga tagagataag agatgggaaa gagtagaact
    17521 tgcatacaat gccatggtta gaggtattgg tgaaaaagca aattctattg aggaagctat
    17581 tcaaaactct tatgatgatg gaaaaaatga tgagtttata atgccaacag ttattatgaa
    17641 agatgataaa ccagttggaa gtataaagga aaatgattca attatattct tcaactttag
    17701 accagataga gctagacaaa taactagagc tttagtttgt gaagagtttg atggatttaa
    17761 aagagaagat attaaaaact tctttgtatg cttaacagag tacgatataa ctatagagaa
    17821 tgtgcatata gcatttggac ctcaatcttt agccaacact ctaggtgagt atctagctaa
    17881 aaatggaaaa actcaactta gagctgctga aactgaaaag tatgcacatg taactttctt
    17941 cttcaatggt ggagtagaag agccaaataa aggtgaagaa agactattga taccttctcc
    18001 aaaggttgca acttatgatt taaaaccaga aatgtcagca tatgaattga cagataaagc
    18061 tttagataaa ttaggagaag ataagtttga ctttatagta ttaaactttg caaatcctga
    18121 tatggtaggt cacacaggaa gtatagaagc ggctataaag gctgttgaaa ctgtagatac
    18181 ttgtgtagga agattgatag ataaaatagt agagttaggt ggaagtgcaa taataactgc
    18241 tgaccacgga aatgctgaat atatgttaga cccagaaaca ggaaaaactg taacggctca
    18301 ctctattaat ccagtaccat ttattgtagt tggtcaagaa tatgaaagtg caaaattatt
    18361 ggatggagga agattatctg atatagcacc tactatttta gatatgatga agttagaaaa
    18421 gccagaagaa atgactggtc attcacttat ttcaaaatag tcgattctga taagtagata
    18481 ataatatata aactacaata taaatttaaa aataatgcag tttaggttta ttttgtaaag
    18541 tttagacttt atgacataag gtttaaattt tgtaagttct ggatattgta aggtgtagat
    18601 gaagatatat tttataaaat ttacaagata gacaatttat aaaataaaaa attacaatat
    18661 agataataat gtaaataatg atataaataa taacaagata gacaataaca atataaataa
    18721 taataattaa tatatattat ataagcaaaa attttagaat cattagttgt acattttatg
    18781 tttaatgttt taaatctagt tataatattt gtgtatttaa gattggtttt ggattttaga
    18841 caagtttttt tatattatat aaattaaaat attataaaaa ataaaataaa aataggaact
    18901 tttacaaatt tacccatcaa ataaatttgt acctaatttt caaaagcaac tttatgtact
    18961 ataatataga aagtagatta aaatagtaga cggatgttta cagtaaatga aaatttaact
    19021 aatagaaaat cgaagggaga tatacaaatg tcagttattg aattagtata tgctagagaa
    19081 gtattagact caagaggaaa tccaactgta gaagttgaag tagtattaga agatggagca
    19141 atgggaagag caatagttcc atcaggagca tctacaggag cttttgaagc tgttgaatta
    19201 agagatggag ataagggaag atacttagga aaaggtgtag aaacagctgt tgctaacgta
    19261 aatgaaataa tagcacctga aatagaagga atggatgcat ttgaccaacc agctatagat
    19321 gcaatcatga tagaattaga tggaactcca aacaaaggta aattaggagc taatgctata
    19381 ttaggtgttt caatggcagt agctagagca gctgctgatg aaataggttt accattattc
    19441 caatacctag gtggagtaaa tgctaaacaa ttacctgttc caatgatgaa catattaaac
    19501 ggtggagagc atgctgataa caacgttgat gttcaagaat ttatgatatt accagtagga
    19561 gcttgttgtt tcaaagaagg tttaagaatg ggagcagaag tattccattc gttaaagaaa
    19621 gttttaggag aaaaaggatt agcttgtggt gtaggtgacg aaggtggatt tgctccaaac
    19681 ttaggttcaa acagagaagc tttagaatta atagttgaag ctataacaaa agctggatat
    19741 aagccaggtg aagacgttat gttaggtctt gacgttgctg ctacagaaat gtacaacaaa
    19801 gaaactaaaa aatacgtttt agcaggagaa ggaaaagagt taactgctgc tgaaatggtt
    19861 gctttatatg aagattggtc aaacaacttc ccaataataa ctatagaaga tggtttagac
    19921 gaagaagatt gggatggatg gaaattatta actgaaaaat taggaaataa attacaatta
    19981 gttggtgacg acttatttgt tactaacaca gagagattag aaaaaggaat tgaaaatgga
    20041 gtagctaact ctatattagt taaagttaat caaataggta caataactga aactttagat
    20101 gctatagaaa tggctaagag agcaggatat actgctgtta tatctcacag atcaggagaa
    20161 actgaagatt ctactatagc agatttagct gttgcagtaa atgctggtca aataaaaact
    20221 ggtgctccat caagaactga tagagttgct aagtacaacc aattattaag aatagaagaa
    20281 atggttggag agcaagctag atattgtgga ttaaaatctt tctataactt aaaaaaatag
    20341 ttattaaaat ggttactaaa aaagattaat gaagagagaa tataagatat ttaagctcat
    20401 cttataagat ataaaagaga taagtgttaa ttgatttttc aattcactta tcctttttta
    20461 tattttatta cttaatttta ttcatagatt cagaaaaaat atgaataaat gtttctatct
    20521 catttttatc taattgcata ggaggttttt attaaaaaat aaaagtgaag acatattttg
    20581 atttaatcat aattgtcatt gaagtattat tgctaaatgt ggattattta ttttaaatta
    20641 tgttgaaatt aaaagttata tcaatatttt aaaatgattg ttttattttg gtggctattt
    20701 ataatatata caatgagagt agattatttt aagtgttaat ttatgtatta atttaaatag
    20761 ttttctcttc taagttttat tcatttttat attgagaata tagtaatata ttatagtaac
    20821 aatatatttt tttgtatgtt atttatagca taatagtctt gaaatggcta tattattgtg
    20881 atacactaaa gagtaatgaa atttaagtaa taaacagata ggaggggcaa gaatgagcaa
    20941 cattttaatg ggtgtgcaag tagtactagg gattttacta gtaattagca taatgcctca
    21001 agattctaag aatgctttac cttcagaatt tggtggagaa ggaagtcaag cttactttaa
    21061 accaaaagga aaacaagcat ttcttgctag agttacaaag ataacagcag tattattttt
    21121 tataaatgca ttggcattac ttatagttaa gaagtagtga tattgttgaa taactggcca
    21181 ttatttaatt agtgcatttg ctagacattt aaaaattaaa tttagttttt aataaaagta
    21241 catcaacata aaaagtacgt aaaataaaag ctggactaat gattttatat gattagtcca
    21301 gtttttattt tatagattga ctatttggtg gcgtttatta tttgtattta tatattgttt
    21361 gtatttatat attttcttca aactaaaaaa tatatagcaa cactactttc accatgttta
    21421 atgagataat atacacaaag ttatattata tagtaaaaat agtataataa tatgttttta
    21481 cgcgattaaa tcagttttat atatgtttct tataatacca atttgtgtta tcaaatatca
    21541 ttaaataaga ttattctgct ttaaaataaa tattattaaa aatactgtaa ttatagaaaa
    21601 tccagaagta aatactacaa tttgtccagc taattcactg tcagaatcca tctgttgtgc
    21661 cattgtaaat gaagaaactg cagttggagc agataacatt atcataagtg aagctaattc
    21721 tacatttcta aatccaagaa aaatgctaat tggtaaaaat atagcaggta caagtataag
    21781 ttttccacat acgcccaata taatgggttt tatatgcccc tttatagcag aaaaactaaa
    21841 agagccgcct agaattataa gtgataaagg agtagctatt ttagaaatat caccaatagt
    21901 tttgtgtaaa aatgtaggta tattaatatt taaaattagc attgtcaatc ctataaaaga
    21961 ggcgattatg agtggatttg ttaagacgct tttaatcata tgttttatat ttactttgcc
    22021 acctctaaaa atttcaagaa ttacaacaga taatacatta aacataggta caataactgc
    22081 aattaataaa gatgtgactc caatactctt atcgccaaat agtgaaattg tcacaggaat
    22141 accaaatata acgaaattgc tacgaaatac accttgtatt aatgcacccc ttgtcttatt
    22201 atctttttca attttaggaa taataaagac taagattaag aagattgaaa aaataattat
    22261 aacagctaca gccataagct taaaattaag agaaccatct aaatctgtat tgtatatatt
    22321 aaaaaataat aataatggca agaaagactt gaagcaaaca ttattcattt tatttaaagt
    22381 atgagtatca aatagaccta atttttttag aacatatcca agtgacattg tgagaaacaa
    22441 aggaagaaca acatttattg ataggattaa attttccaag gtttactctc cgtgattcta
    22501 tgatttgata attcaattaa atttccatta gtttttttca aataaataga tttcatatta
    22561 ccaaagacac ctgtacattc tacaattcct ctcttaattt gaatctcatt ttgttctaag
    22621 tatttttttg gctcatcaat atttgtgttt attttaaaac ataagttatc tctacttgct
    22681 tttgaatttt taacataagg taaaaattta taacctttta tgttaagatt tatcttaaca
    22741 tttctagcat taagtttata atttttgctc atttctttag atgaaaaacc aaatttttta
    22801 taaaaattga tataattttc tatattatta ttagtaatga ccacatgttc tatttttttt
    22861 atcatggcaa ctccttaaga ttttatataa attagttaaa tttatataag ttattaaatt
    22921 ttatatagat tgatga
//
LOCUS       DAYAPA010000060        22567 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.060, whole genome shotgun sequence.
ACCESSION   DAYAPA010000060 DAYAPA010000000
VERSION     DAYAPA010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22567
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.060"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            230..1390
                     /locus_tag="ACGI59_002109"
     CDS             230..1390
                     /locus_tag="ACGI59_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HFG9739895.1"
                     /translation="MIETGFVYCSFLVVFVGVIFFMVDKLKWKVFDVINPMLLIYLGS
                     AILGSFGLFAQNEDVGTYQNIITVNFLPLMLVFLLIQCDLRKILKMGPKMLLSFFCAT
                     FTFMLGFVIAFIIFKNSMGADSWKILGAASGSWIGGSSNMIAAANALGVAEEGLSYAI
                     LMGSIGYTVWMSVCLMSVKFGPKFNKWTKCDTSFIDEVTSKLEKENKDNTAPTFVELM
                     ILLSVGFGVASFAQIVSKILPTSGILNETMWVVLVASTVSILLGMTKISKLKGSTLVG
                     NVFMYLLLAATGSKANFFGLSEAPVYIMFGLVVVLVHAISFIGLAKLFKLDLFTCEVG
                     SIANIGGVTTAPMIAGVHNPVLIPVGVLMGLLGNIIGTYCALMVTQILHMLA"
     gene            1732..1893
                     /locus_tag="ACGI59_002110"
     CDS             1732..1893
                     /locus_tag="ACGI59_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9739896.1"
                     /translation="MIIPSRRIQDITLTNLKNGEVTLIELDEIYKKMGFLFVVSEGKL
                     KKIKKENHH"
     gene            2086..2715
                     /locus_tag="ACGI59_002111"
     CDS             2086..2715
                     /locus_tag="ACGI59_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFG9739897.1"
                     /translation="MKIVTYNIHKGMDSNNRLTLTKMGLYLKQLDCDVICLQEVLYPQ
                     FLALKAVLNMDGVFATNVKKVNMIYGICTFTTSKMLNSNHFFLTSKKEQRGALCITID
                     AYGRIINVINTHLGLDRQERAKQLDEIIDYRNRLVGIVILCGDFNEKNVFLSMFNDMA
                     ISLNKSYLPTFEKSNSRIDYIFVNKNTELKGYTVEKIYLSDHYPVIGYI"
     gene            2954..4213
                     /locus_tag="ACGI59_002112"
     CDS             2954..4213
                     /locus_tag="ACGI59_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HFG9739898.1"
                     /translation="MGRRTTRVSRLGKEKKRSVLIVLAALVLIMGGFTYFFNSKFLYN
                     GKIAKNVYIEGVNVSDMTKAEALKAITDKYTPEDLNLAYDGKKYTISPKDIDLKYDTE
                     DVVKDAYESTKKGSYFQNLKKYIDIRVNKANMKIKAEYDEAKLSSKVSSIADSINVKM
                     KNASISVGSGGLSYTDSVVGREFDLAANKESIYNMIKNKEHKTLELKVNLQKPDITTE
                     QVKTVNSVIGQYSTTYSQAVEGRSYNVGLSARKTSDVLLMPGEEFSYNKLTGPSNKAN
                     GYKDAPVIVYGKLEQSAGGGVCQTSSTVYNAALLSGMEITQVTNHSSASTYVPKGRDA
                     TVSDGGLNLKFKNPYKHPVYIKNYAGGGSVSSVIYGNSGDKPNISIEVKQTGQNKYST
                     YRIFKDSSGKVIKKEHISNSSYKELKK"
     gene            4484..5467
                     /locus_tag="ACGI59_002113"
     CDS             4484..5467
                     /locus_tag="ACGI59_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HFG9739899.1"
                     /translation="MTWLNFIGNRYEVVNSDDVLEINKIYKARDVFYRKNVLIKVIKH
                     NNYICEDFVSNLIDESTALDEINSPYILKIIDVGIHCTEETTLYYVVSEDFNGIGLDE
                     LILGNYLHLEAIVNIMIQVLKALEMIHRNHSYHGSLKSSSIIVDTEYNIKICDFGITK
                     ANNGVNTRSYGNRRYLCPHQLCINYTDKESDFFATGLILFESIFKKLPFGESNSEEKM
                     LQSIDKGLDWNSIKAINGNTELINVIKRLLGRNNKYTRANDIIIDLSKVMYEKAYIEV
                     EDEKEEEQKQDKVIQVKKEYKSKMRKKLHKKLVVAGLAIVMISMIIISSII"
     gene            5713..6780
                     /locus_tag="ACGI59_002114"
     CDS             5713..6780
                     /locus_tag="ACGI59_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HFG9739900.1"
                     /translation="MSNIKIIASNKCWIEDIARKQLEDISKLNGILRVVGLPDLHAGK
                     IPVGLAVETKNIIYPHIIGNDIGCGMTLFKTGVLKKKFKKDKWIKSLSKIKDLSDIEI
                     KNTYKEECPILNLGTIGSGNHFAEIQCISEIYNKEQFEQLKFSSDDIMMLVHCGSRNY
                     GEDILKKFYDKDGLEVESEKAIDYIKKHDNALMWAERNREAISKKIMQSIGTSGDVET
                     IFSINHNFIEKREDKFIHRKGAVSSERGAVIIPGSRGSLSYIVMPTENTEISLYSLSH
                     GAGRKWSRSVCKSRLKSKYSKDTIKQTKFKSQIICNDVNLLFQEAPEAYKNIEQIIES
                     LIEYELIQVVATMKPLITYKG"
     gene            7314..9224
                     /locus_tag="ACGI59_002115"
     CDS             7314..9224
                     /locus_tag="ACGI59_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HFG9739901.1"
                     /translation="MNSNTFERLQLNEVKDLIKIHCVSSLGKNLIDKLTPSGNIGVVR
                     RKLAENKEARKIIENSNHIPLEGLFNVGSTIDKIEKGMIIEPVELVNIEDFLRGCRKM
                     KAFMLEKEFYSPTLSSYALNITECKSIEDEINYCIKSNKVDSNASKELKKIRRNIEIT
                     EGKIKDRLNKFITSTVNKKYIQEFIISKRNDRYVIPIKSSYKNEVNGTILDTSSKGNT
                     VFIEPISVSNLSTELTMLKADETIEEYKILSYLTELIFEKISQIKLNIEILSEYDMVF
                     AKAKYSQKIKGITPKINNNGYIKIIKGKHPLLTGDAVPLDFEIGKNYRSLIITGPNAG
                     GKTVTLKTVGLLTLMVQCGFDISAKEGSEFSVFEKVFVDIGDNQSIENALSTFSSHIK
                     NIAEIMSLSNNSTLVLFDEIGSGTEPNEGAGLAISLLEEFYKMGCITIASTHYGEIKK
                     FATLHPEFENAGMMFDKETLEPLYKLTIGKSEDSNALFISKKMGIKNRVLDRAKEYVI
                     DRNYNLDLVKRSKLQSNIEDKNSSEVILNYTDYNVGDKVKLLDEDDFGIVYKPMDKFN
                     NVEVLFNDNFISVNIKRLQLSIKASELYPEGYDLDVLFTSFKERKLEKDIQRGSKKAL
                     KKIQKEIRENRK"
     gene            complement(9287..9910)
                     /gene="ytaF"
                     /locus_tag="ACGI59_002116"
     CDS             complement(9287..9910)
                     /gene="ytaF"
                     /locus_tag="ACGI59_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation membrane protein YtaF"
                     /protein_id="HFG9739902.1"
                     /translation="MIESILLVTSLCIDAFVTSFGYGVNKVKVPLYSNIIISVVCSAI
                     LAISLFAGTIVRDFLPQNITMLICFFILFLLGSARLIESFFKSYFKKKSIHSKNYNFN
                     MLDLKLNFNVSIEPDVLDTSKSKVLKSAEAFTLALALSLDGLAIGFGSGLVGVNYIQV
                     ILFSLISNIIAVLLGCLLGNMFVKNVNLNLSWLSGLILLVIGFMKIC"
     gene            10098..10742
                     /locus_tag="ACGI59_002117"
     CDS             10098..10742
                     /locus_tag="ACGI59_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFG9739903.1"
                     /translation="MEQNNKVGNFGDLLKILLDEKELSMGELSKKSGIDKSTISRIAN
                     NKQKPNINHLEKMAIHLNINLEELLKASGYEFRNSNNQIFNADSDFSNFDDILGFANL
                     INDKNFNGNIEKELSKCKLYVQTDEGKKLLFDNFNKKIDSIEKQGQFTDRLRQMYADF
                     CTDGLTIKKYLLIGSMLLYFVISTDVIPDFVFPIGFMDDLVALNIVTKLLKNDK"
     gene            10956..12014
                     /locus_tag="ACGI59_002118"
     CDS             10956..12014
                     /locus_tag="ACGI59_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HFG9739904.1"
                     /translation="MNCKWISKIDERKKCHREADSSGYCIFHKENKSDEEIQLMMDTI
                     HKEEISEFNGFVFENEFNAEEILTYNYKILDFSESIFKQKANFKKYIFKKNIIFNYTE
                     FRDKVLFNGCVFLENCDFNRSIFSKNYINDRIFEKVKFKGPDLVVNKVENFPRMDGII
                     FSMCTKFVLKNVEYGKSEYEHGKINYRIARNQATKIGEYEMIGFYYYKERIYSSKIMK
                     RSNYPTFSDYLVEKFFDQIARYTTGYGEKPWNILLVIIAIISVFALLYLFVGIESSNS
                     TLVALDINNIGDYSLSEIFKMYMDLWYFSMATFSTVGYGDMVATSLIGKALAGIEVFF
                     GVTIGAIWASVIIKRMIR"
     gene            12118..13302
                     /locus_tag="ACGI59_002119"
     CDS             12118..13302
                     /locus_tag="ACGI59_002119"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFG9739905.1"
                     /translation="MKNALKKIISLAMILVFIIPNYSYADDKSVDKYSKTSILIDQET
                     GRVLYSKEPDMKVPLASLSKMMTFLLAIEAIENKEVKETDKVKIDKSIASVKGSSYKL
                     KDGEEVPLIELMRGLMIVSGNDAAIAIAKHICKTKEAFVDRMNKKAKEIGMSNTHFLN
                     PNGLPIYDLSNPKKPAKENISTAKDIAILGKYMFDHYEKQVTAITDMETYTNSDRSFE
                     KSNTNALLRIIPEVDGIKTGYTGNAGYCLSFSMIVNKNDKNEKKRRVIGVVLGTNHKN
                     KRTSASLALLKYGKENFNMKKVIDKDSFIGKKYIKGMKELEVTLKAKDELYTILKDDE
                     SIKSEVKIKNIEYPVKKGDTMGIIKYYTNSGDLLGSVDIVSNNSIDNVSLKTKLKMKF
                     AN"
     gene            13509..14222
                     /gene="rpsB"
                     /locus_tag="ACGI59_002120"
     CDS             13509..14222
                     /gene="rpsB"
                     /locus_tag="ACGI59_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFG9739906.1"
                     /translation="MSVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIYIIDLQ
                     KTVKKVEEAYKFTKEVAETGKPILFVGTKKQAQDAIKDEAERCGMYFVNERWLGGMLT
                     NHKTIKTRINKLRELEKMEEEGVFNVLPKKEVIKLRAEKEKLEKYLGGIKDMPELPGA
                     MFVVDPRKENIAIQEAHRLGIPVVGIVDTNCDPEQLDFAIPGNDDAIRAVKLITGAMA
                     TAVIEGRQGAEEEVAEDQE"
     gene            14301..15212
                     /gene="tsf"
                     /locus_tag="ACGI59_002121"
     CDS             14301..15212
                     /gene="tsf"
                     /locus_tag="ACGI59_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFG9739907.1"
                     /translation="MANITAQMVKELRESTGAGMMDCKKALQEAEGNMEKAVDLLREK
                     GLSKAAKKAGRVAAEGLVAIEMNDDNTVASMVEVNSETDFVAKNEDFKVFVKDAACMA
                     LATDKEDIASLLGETHKEGITLQEVLNNRVAKIGEKLDFRRFAKVVTNGQVAGYIHGG
                     GKIGVLVEMETEARDAKVLELGKDVAMQVAAMNPKYVSRDEVDAEYIAHETEVLTQQA
                     LNEGKPANIVEKMVKGRLEKELKEVCLLEQTFVKNPDITVKQLVADVAKAVGSDIKVV
                     KVVRFEVGEGIQKREENFAEEVAKQLK"
     gene            15297..16001
                     /gene="pyrH"
                     /locus_tag="ACGI59_002122"
     CDS             15297..16001
                     /gene="pyrH"
                     /locus_tag="ACGI59_002122"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFG9739908.1"
                     /translation="MDKPMYKRVLLKLSGEALAGERGFGINNDVVNDIAIAIKKIQEI
                     GVEVAVVVGGGNFWRGRTSEGMDRTTADYIGMLATVMNAMALQDALENIDVATRVQTA
                     IDMRQIAEPYIRRRAVRHLEKERVVIFGAGTGNPYFTTDTTAALRAAEMEAEVILLAK
                     NVDAVYDKDPKVHADAKKFTELSYMEVIQKELKVMDSTATSLCMDNKIPIKVFELTTE
                     NIIRAVKGENIGTTVK"
     gene            16027..16584
                     /gene="frr"
                     /locus_tag="ACGI59_002123"
     CDS             16027..16584
                     /gene="frr"
                     /locus_tag="ACGI59_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFG9739909.1"
                     /translation="MKLEIHKQLEEKMNGTIDALKFEFGTIRAGRANAQMLDKIRVDY
                     YGTPTPINQIGAISVPEPRILMISPWDKSAMHEIEKAIANSDLGLNPSNDGEVIRLSV
                     PALTEERRKELAKKASKAAEEFKVRIRNERRDANEKIKKMEKGGELTEDELKKAQDEV
                     QKMTDKFIKEIDALLSKKEKDIMEV"
     gene            16587..16790
                     /locus_tag="ACGI59_002124"
     CDS             16587..16790
                     /locus_tag="ACGI59_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9739910.1"
                     /translation="MKKYYNLLGLHVDEVKQYFDEQNINYTVKSIEGKKDKEKLVVPR
                     VIKISEIGDSVELIITYFSDSLV"
     gene            16819..17553
                     /locus_tag="ACGI59_002125"
     CDS             16819..17553
                     /locus_tag="ACGI59_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HFG9739911.1"
                     /translation="MNNNIIYDIDLNNIPTHIAIIMDGNGRWAKARFLPRTAGHKAGV
                     ETIRDIVKECSKLGVKHLTLYAFSTENWKRPKLEVDTLMNLLSTYLRNEIAELHQNNV
                     KVTAIGDISALPKTCIRELNSAKELTKNNTGVNLNLALNYGSRADIKNALIDIVKNCE
                     SGKIDINNIDEDIIKNYLSTKSIPDPDLVIRTSGEQRLSNFLLWEVAYSEFYFTDIHW
                     PDFKKEELQKAIYVYQRRDRRFGGLK"
     gene            17566..18345
                     /locus_tag="ACGI59_002126"
     CDS             17566..18345
                     /locus_tag="ACGI59_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFG9739912.1"
                     /translation="MLTRIIASLALVPLFLFVVYGGIPLYIAETAIVYIALHEFYKAF
                     KIKDVHPIFIIGYLFSIYLAVKNIFNLPLEYTYAVIFILFLASIIYMLMGKNNVIDVS
                     ITFLGVFYIGVFLDFIIITINGFEKGSIYVWLIFVISFMTDIFAYFSGYLLGKHKLIP
                     KVSPKKTIEGAIGGIIGSTLCCILFGYLFGIDLLQLAIIGSIGSVIAQLGDLFASSIK
                     RYVGIKDYGKIIPGHGGILDRFDSVILVAPFVYSAIKFFIR"
     gene            19169..20980
                     /locus_tag="ACGI59_002127"
     CDS             19169..20980
                     /locus_tag="ACGI59_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFG9739913.1"
                     /translation="MKKKYDFLIYSAIWLVIIILFIFSLYHSIEHIKIISHTGTIRSE
                     TQKVVKQELNNERNDDLIKRLDNILIKLRTGNGENGFQRCDNKEFQQKLNQMDSMWES
                     MKKEIIKVRNGASGDKLYKLSEEYSVLSNQIVFISEKHSNAKLYSFATALFIYLIFST
                     ISLLIWEYYNKKRFKRIFYTDNLTKIKNQVAFENRAIEILHNASNKEYVLLNIDIDNF
                     KYINDTHGYEYGDKVLIIVATVLSKTFNIKETCARICSDNFVILAKYRDSLLEDIREM
                     LTNAIISELDMNVTQTISYCIGAYLVEIDNLGYKSINSMMDKANIAHKVSKTRGISST
                     VWYNENLLKQLQMENSIYNYMYKAIENEEFHMYLQPKFQISSLNVVSAEALVRWFSPE
                     LGFLSPDEFIPLFEKSGFIIELDFYMLKKACSFVKKTFMKKNQYTYPIAVNFSRVTIY
                     QNSFYQRFLDTVKEYEIPFKYIEIEVTESAFNEISQPVISILEELKKLGFLISMDDFG
                     SGYSSLSLLCSLSINGLKLDKSLLKETFNREKVYSIIQCIIEMSHRIGMSVVCEGIET
                     KKDLEFLNTVKCDVGQGFYFSKPIEEKEFFNKYVTKK"
     gene            complement(21068..21649)
                     /locus_tag="ACGI59_002128"
     CDS             complement(21068..21649)
                     /locus_tag="ACGI59_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9739914.1"
                     /translation="MSYLLKINQFERSYDIGIFKNEQSIYKFIEKIPFVKKDSSNFNY
                     TNYYMEFEAIPEYYEVNFNNYLYIITRFSFIPDGTEIFFTWEEIHCWDEKVATEKTFI
                     NGETLVDSYNFPNNEVKDYIQKRESLYIETKKYYEEKGLKIERHALGSEDGEYVELTN
                     DSILYLLDIEAVEIREQSKNIEEFLYKYKKRFK"
     gene            complement(22085..22291)
                     /locus_tag="ACGI59_002129"
     CDS             complement(22085..22291)
                     /locus_tag="ACGI59_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9739915.1"
                     /translation="MNQSQEKDLEFIKNHWDLTIIEKERNVTRIIRYSLIILAIIAFF
                     LPTKIKFSIYIFISLFCWLMYLVL"
BASE COUNT         9171 a         2234 c         3757 g         7405 t
ORIGIN      
        1 attttagtaa taaaataatt gttgaaaaag attttaaatt cagattattc atttctaaag
       61 taattaatgt gcaaatcttt attaatatat gtcaatagtt aggtatatat gtatttaatt
      121 agcatgatat agataaaagt atttatttta attgtctatt atctgaaaaa gtctttactt
      181 agaaatcata aaaatcaaat taattaggaa aagaaagggt gtgtataatg tgattgagac
      241 tggatttgta tattgtagtt tcttggttgt attcgtaggt gtaatttttt ttatggttga
      301 taaattgaaa tggaaggtat ttgatgttat taatccaatg cttttaatat acttaggttc
      361 agctatactt ggatcatttg gtttatttgc acagaatgaa gatgttggaa cgtatcaaaa
      421 cataataaca gtgaacttcc ttccattaat gttagtgttt ttgttaattc aatgtgattt
      481 aagaaaaata ttaaagatgg ggcctaaaat gttattatca tttttctgtg ctacatttac
      541 atttatgctt ggatttgtta tagcattcat aatttttaaa aattcaatgg gtgcagattc
      601 atggaaaatt cttggggcag catctggttc atggataggt ggctcatcaa atatgattgc
      661 agcagctaat gctctaggag ttgcagaaga aggacttagt tatgcaatac ttatgggttc
      721 tataggatat acagtatgga tgtctgtttg tctaatgtct gtgaaatttg gacctaaatt
      781 taacaaatgg acaaagtgcg atacaagttt tatagatgag gttacatcaa aacttgaaaa
      841 agaaaataaa gataatacag cacctacgtt tgtagaactt atgattttac tttctgttgg
      901 gtttggagtt gcgtcttttg cacaaattgt atccaagata cttccaactt caggtatact
      961 taatgaaact atgtgggtag tacttgttgc atctacagta tcaatattat tgggtatgac
     1021 aaaaatatca aagcttaaag ggagtacact ggtaggaaat gtgtttatgt atttactcct
     1081 tgcagctaca ggtagtaaag caaatttctt tggattgtca gaagcacctg tttacataat
     1141 gtttggattg gtagtagtcc ttgtacatgc tatttcattt atagggcttg ctaaactatt
     1201 taaacttgat ttatttactt gtgaagtagg aagtatagct aatataggag gagtaactac
     1261 agctccaatg attgctgggg ttcacaatcc tgtattaata ccagtaggtg tacttatggg
     1321 gcttctagga aatattatag ggacttattg tgctcttatg gtaactcaaa tattacatat
     1381 gttagcttaa taaaaatatg gtataaaatt aataatttaa ataagaaaag tcatttcaat
     1441 ttaagcagaa gcttttactg aaatggcttt tttgtttgac aagataatct atttcaagaa
     1501 gatgttttga catatttagt gtataatatt gtaaaattta ctaaaaacga gatatattta
     1561 atttttatat tgaatcgaac gtctgtatga tgtataataa atatacagaa cgaatgtttg
     1621 cagaacgggt gttttttgaa tttattttaa taatttatga atatatatca gaatgcataa
     1681 atatatttaa atataaagat taaaaagaat ttaaaggagt gttgataact tatgataatt
     1741 ccatcaagaa gaatacaaga tataacatta acaaatttaa aaaatggtga agtaacttta
     1801 attgaattgg atgaaatata caagaagatg ggatttttat ttgttgttag tgaaggcaaa
     1861 ttgaaaaaga taaaaaagga gaatcatcat tagaaatgaa tgaaataata aatattataa
     1921 tagtttataa aacctaactt atgtagaata taaatactta agttaggttt ttttgtgata
     1981 aagtttataa gtactcaatt ttataagata aataatggtc ttaagtgaaa agtaaataag
     2041 tttaaatttt agtaagtatt atattgcata agaggtgata ttattatgaa aatagtaacc
     2101 tacaatattc ataaaggtat ggattcaaat aatagattaa ctctaactaa aatggggtta
     2161 tatttaaaac aattggattg tgatgtaatt tgtttgcaag aagtattata tcctcaattt
     2221 ttagctttaa aagctgtttt aaatatggat ggtgtctttg cgacaaatgt aaaaaaagta
     2281 aatatgatat atggtatttg tacatttaca acatctaaaa tgttaaacag taatcatttt
     2341 ttcttaacta gcaaaaaaga acaacgggga gctctttgta taactataga tgcatatgga
     2401 agaattataa atgtaataaa tactcacttg ggacttgata ggcaggagag agcaaaacaa
     2461 ttagacgaaa taatagacta taggaacaga ttagtgggga tagttatttt atgtggtgat
     2521 tttaatgaga aaaatgtctt tttaagtatg tttaatgata tggctatttc actaaacaag
     2581 tcttacttac caacatttga aaaatcaaac tctagaatag attacatttt tgttaataaa
     2641 aatactgaac taaaaggata cacagtagaa aaaatttacc tgtcagacca ttatcctgta
     2701 ataggttaca tttaaatttc aatataaatg aaattagttg ttataaaata aacaattttt
     2761 tgttaagaaa tagtaaaaga aatataataa aaaatatcaa aaataaaact aattataaga
     2821 cataaattta caataaagtt acatttgatt aaattccttg tttaacaaaa gctattcaaa
     2881 tataatctaa ggtagttttg aaatgggaac tctattatgc aaatttaagc tagtgttaaa
     2941 atgaggtgaa gttatgggca gaagaacaac aagagtttca agattaggta aagaaaaaaa
     3001 acgaagtgtt ttaattgtat tggcagcttt agttctaata atgggtggat ttacttattt
     3061 ttttaacagt aaatttctgt acaatgggaa aatcgctaaa aatgtatata tagaaggagt
     3121 aaatgtatct gatatgacta aagcagaggc attaaaagct ataacagata agtatactcc
     3181 agaagattta aatcttgctt atgatgggaa aaaatataca atatcaccaa aagatattga
     3241 tttaaaatat gatacagaag atgttgtaaa agatgcttat gaaagtacaa aaaaagggtc
     3301 ctattttcaa aacttaaaaa aatatattga tataagagtg aataaggcaa atatgaagat
     3361 aaaagctgaa tatgatgagg caaaacttag ttccaaagtg tcttctatag cagatagtat
     3421 aaatgttaag atgaaaaatg cttctattag tgttggaagt ggtggactaa gttatacaga
     3481 ctctgttgta ggtagagaat ttgatttggc agcaaataaa gagtctatat ataatatgat
     3541 aaaaaataaa gagcataaaa ctttagaact taaagtaaat cttcaaaaac cagatataac
     3601 aactgaacaa gtaaaaactg taaattctgt tataggtcaa tattcaacta cttattcaca
     3661 agctgtagag ggtaggtcat ataatgtagg attatcagca agaaaaacta gtgatgtatt
     3721 attaatgcca ggtgaagaat tttcatacaa taaacttaca ggacctagta ataaagcaaa
     3781 tggttacaaa gatgcacctg ttatagttta tggtaagcta gaacaatcag caggaggagg
     3841 tgtttgtcaa acatcttcaa cagtttataa tgcggctctt ctttcgggta tggagataac
     3901 tcaagttaca aatcattcat ctgcatctac ttatgtgcct aaagggagag atgctactgt
     3961 gagcgatgga ggattaaact taaagtttaa aaatccatat aagcatcctg tatatattaa
     4021 aaattatgca ggtggaggaa gtgtatcatc agtaatctat ggtaattctg gtgacaaacc
     4081 aaatatatct atagaggtaa aacaaactgg tcagaataaa tattctacat atcgaatatt
     4141 taaagattca agtggaaagg tcataaaaaa agaacatata tcaaatagtt cttataaaga
     4201 attaaaaaaa tagataattt tttagagaaa tatttgtaat ataaaaggga gtgtctcaaa
     4261 atgaactttt tagtttatga gacactcttt ttctgtgaaa tcaaatatat tttcatcatt
     4321 tcaacaatac aaaagaagcg tatctctatt ttgagacagc cttttttgta aaaattaaca
     4381 ctattagttc ttgttttaat attacaatca ttaatatata gaaaatattc ctatatattg
     4441 gtataattgt aaatagattt agttatataa aatgtatgag gaggtgacat ggttgaattt
     4501 tataggcaat agatatgagg tagtaaattc tgacgacgtt ttagaaatta acaaaatcta
     4561 taaagctaga gatgtttttt acaggaaaaa tgtgttaata aaagttataa agcacaataa
     4621 ttatatctgt gaagattttg tttcaaattt aattgatgaa agtactgctt tagatgagat
     4681 aaactctcct tatatactaa aaataataga tgttgggatt cactgtacag aagaaacaac
     4741 attatattat gttgtaagtg aagactttaa tggtatcgga ctagatgaat taattctagg
     4801 taattatctt catttagaag caattgtaaa cataatgata caagttttaa aagctttaga
     4861 aatgatacat aggaatcatt catatcatgg tagtttaaaa tcgagtagta taatagtaga
     4921 tacagaatac aatataaaaa tttgtgattt tggaattaca aaagcaaata atggggttaa
     4981 tacaagaagc tatggaaaca ggagatattt atgcccacat caactatgta taaattatac
     5041 tgataaagaa agtgattttt ttgcaacagg tcttattcta tttgaatcta tatttaaaaa
     5101 attgcctttt ggagaatcaa attcagaaga aaaaatgcta caatctattg ataagggatt
     5161 agattggaat agtataaaag caattaatgg aaatactgaa cttataaatg ttataaaaag
     5221 gcttcttgga agaaataata agtatactag agccaatgat attattattg atttaagtaa
     5281 agtaatgtat gaaaaagcat atatagaagt tgaagatgag aaagaagaag agcagaaaca
     5341 agacaaagtt atacaagtta aaaaagaata taaaagtaaa atgagaaaaa aattacataa
     5401 aaaacttgtt gttgccggtc tagctatagt catgatttca atgattatta ttagttccat
     5461 aatataaaaa ttgtatatta agtattttaa aatcaatgtc tataaaggat atacatagaa
     5521 attttagtgt agtcttaaat tatgaaagga agtggttatt atgttaacaa aaaataatga
     5581 ggtattaaac gtctaggagt attttaacaa atagccacct gcttttacag tttagaataa
     5641 aaaagctgtg gctaaaggtc acagcttttt gctacttttt aaaggcagga gaatattaaa
     5701 aaggagcaaa ttatgagcaa tataaaaata atagcaagta ataaatgttg gattgaagat
     5761 atagcaagga aacaacttga agatatttca aagttaaatg gaattttaag agtagtagga
     5821 ttacctgact tacatgcagg taaaatacct gtaggtttag cagttgagac taaaaatatt
     5881 atttatccac atataattgg aaatgatata ggatgcggaa tgactttatt taaaactggg
     5941 gttctaaaaa agaaatttaa aaaagacaaa tggattaagt cacttagtaa gattaaagat
     6001 ttgtctgata tagaaataaa aaacacatat aaagaagaat gtccaatttt aaatttagga
     6061 actataggga gtggaaatca ttttgcagaa attcaatgta taagtgaaat ttataataaa
     6121 gaacaatttg aacaattaaa attttcatct gatgatatta tgatgttagt tcactgtggg
     6181 tcaagaaatt atggagaaga tattttaaaa aaattttatg ataaagatgg acttgaagtt
     6241 gaaagtgaga aagctataga ttatattaaa aaacatgata atgctttaat gtgggcagag
     6301 cgtaatcgtg aagctatcag taaaaaaatt atgcagtcaa taggtacatc tggagatgtt
     6361 gaaacaatat tctctataaa tcataatttt atagagaaaa gggaagataa atttatacat
     6421 agaaaaggtg ctgtttcaag tgaaagagga gctgtgatta taccaggttc aagaggcagt
     6481 ctatcatata tagttatgcc tactgaaaat acagagatat ctttatattc tctatcacat
     6541 ggagcaggta gaaaatggtc tcgttcagtt tgtaaatctc gccttaaaag taagtatagt
     6601 aaagatacta taaaacaaac taaatttaaa agtcagatta tatgtaatga tgtaaattta
     6661 ttatttcaag aagctccaga ggcatataag aatatagagc agattattga aagtttgata
     6721 gaatatgaac ttattcaagt agtggcaact atgaagccat tgattactta taaaggataa
     6781 tttttaaagt taaaaaagat taaaattata ttataaatag ctaattttat ggaacattta
     6841 aaagtgtttt attattagct atttttttat tttgtagaga cgataattta tagagctaaa
     6901 ttctttgata tataatgatt acaaagttag actgtattgg aaactgcaca tacatgaatt
     6961 gatgacaata taagtgattc ccaaatataa atgaaagtat aagcttattt ttactataaa
     7021 aattttcact gtacaaaaac attaatgaca ttatttttat tgacataaat atataattta
     7081 tggaataata ttagaagcac tgatataagt gtttaaataa gaatagaaaa ggagaagtgt
     7141 tttatgaaaa aaaatactaa cattaatgag tccggagtta aacttcttaa taaataagga
     7201 gtttgatata taataactcc ttatttatta agttgttcgc aaaataacta ttatcaaagt
     7261 aaatgatagg gtgatttaaa agacgatgac ttataaataa aggagaaaaa ataatgaata
     7321 gtaatacatt tgaaagatta caactaaatg aagttaaaga cttaataaaa atacattgtg
     7381 taagttcgct aggaaaaaat ttaatagata aattaactcc tagtgggaat attggagtag
     7441 taagaaggaa attagcagaa aataaagaag ctagaaagat aattgaaaat agtaatcaca
     7501 taccacttga aggattgttt aatgtaggtt ctacaataga taaaattgaa aaaggaatga
     7561 taatagaacc tgttgaactt gtaaatattg aagatttttt aagaggttgt aggaagatga
     7621 aagcttttat gttagaaaaa gaattttatt cacctacatt aagttcttat gccttaaata
     7681 taacagaatg taaaagtata gaagatgaga taaattactg tataaaatct aataaagttg
     7741 attctaatgc tagtaaagaa ttaaaaaaga taagacgaaa tatagaaatt acagaaggaa
     7801 agataaaaga tagattaaat aaatttataa catcaactgt aaataaaaag tatatacaag
     7861 agtttataat aagtaaaaga aatgatagat atgtaatacc aattaaatct tcttataaaa
     7921 atgaagttaa tggtactata ttagatacat catcaaaagg aaatactgta tttattgaac
     7981 ctattagtgt ttcaaattta agtacagagt taactatgct aaaagctgat gaaactattg
     8041 aagaatacaa aatattatct tatttaacag aacttatatt tgagaagata agtcagataa
     8101 aattaaatat agagatactt tcagaatacg atatggtatt tgcaaaagct aagtatagtc
     8161 aaaaaataaa aggaattact ccaaaaataa ataataatgg ctatataaaa ataattaaag
     8221 gtaaacatcc acttctaaca ggagatgcag tacctttaga ttttgaaata ggcaaaaatt
     8281 atagaagtct tataataaca ggtcctaatg caggaggaaa aactgtaacc cttaagactg
     8341 ttggtttact aactttaatg gttcaatgtg gatttgatat ttctgcaaaa gaaggtagtg
     8401 aatttagtgt atttgaaaaa gtttttgtag atatagggga taatcaaagt attgaaaatg
     8461 ccttaagtac tttttcatca catataaaaa atattgcaga gattatgagt ttatcaaata
     8521 attctactct tgttctgttt gatgaaatag gaagtggaac tgaacctaat gaaggagctg
     8581 gtttagcaat atcattatta gaagagtttt acaaaatggg atgtataact atagcatcaa
     8641 ctcattatgg agagataaaa aagtttgcta cattgcatcc agaatttgaa aatgctggta
     8701 tgatgtttga taaagaaacg ttagaaccat tgtataagtt aactattgga aaatctgaag
     8761 atagtaatgc tctttttatt tctaaaaaaa tgggaataaa aaatagagtt ttagatagag
     8821 ctaaagaata tgtaattgat agaaactaca atttagattt agttaaaaga agtaagctac
     8881 aatctaatat tgaagataaa aatagttctg aagtcatttt aaactataca gattataatg
     8941 taggggataa ggtaaaatta ctagatgaag atgattttgg aattgtatat aaacctatgg
     9001 acaagtttaa taatgtagaa gtattgttta atgataattt tatttcagta aatataaaaa
     9061 gattacaact tagcataaaa gcatcagaat tgtatccaga agggtatgat ttagatgtat
     9121 tatttactag ttttaaagaa agaaaattag aaaaagatat tcaaagaggt tcaaaaaaag
     9181 ctcttaaaaa gattcagaaa gaaattagag agaatagaaa ataaatataa aaagcacaaa
     9241 agaaagcaat ctaaagagaa aagtttatct ttagattgct ttcttattaa catatcttca
     9301 taaatccaat gactaacaag attaaaccac ttaaccaaga taagtttaaa tttacgtttt
     9361 taacaaacat atttcctagt aaacaaccaa gtaatactgc aattatattt gatattaaag
     9421 aaaatagaat aacttgtata taattcacac ctacaaggcc actaccaaaa ccgatagcta
     9481 atccatctaa acttaaagca agtgcaagtg taaatgcttc agcagattta agcactttag
     9541 atttacttgt atctaagaca tcaggttcga tacttacatt aaaatttaat ttaaggtcta
     9601 acatattaaa attataattc tttgagtgta tagatttttt cttaaaataa gatttaaaaa
     9661 aactttcaat tagtctagca cttcctaata aaaataaaat aaagaaacat attaacattg
     9721 ttatattttg aggtaaaaag tctctaacta ttgtacctgc aaaaagcgat attgctaaaa
     9781 tagctgaaca aactacgctg ataattatat ttgaatataa gggaacttta actttattta
     9841 cgccatatcc aaagcttgtc acaaaggcat ctatacaaag agaagtaact aataaaattg
     9901 attcaatcac agtagattaa catcctttct cttaaatttt gtacttatat tagttatagt
     9961 attgtaaaaa tggataaatt gttacagata atccaataaa ttgttgataa aattccaaca
    10021 tcatacaaca cctaaataac aaaagaatca tatcatataa taatataaaa ccaatcataa
    10081 taaagcgggt gaaaataatg gaacaaaata ataaggttgg gaatttcggt gatttgttga
    10141 agattttatt agatgaaaaa gaattatcaa tgggagaact aagtaaaaaa agtggaattg
    10201 ataaatctac tatatctcgt atagctaata ataaacaaaa gccaaatata aatcatttag
    10261 aaaagatggc aatacattta aatattaacc tagaagaact gttgaaagct tcaggatatg
    10321 aatttagaaa cagcaataat caaatattta atgcagatag tgattttagt aactttgatg
    10381 atattcttgg atttgcaaat ttaatcaatg ataagaactt taatggaaat attgaaaaag
    10441 agttaagtaa gtgtaagtta tatgtccaaa cagatgaagg taaaaaatta ctttttgata
    10501 attttaataa aaaaatagat tctattgaaa aacaaggtca atttacagat agattaagac
    10561 aaatgtatgc tgatttttgt acagatggac ttacaataaa gaaatattta ttgattggaa
    10621 gtatgctact ttattttgtt atatcaacag atgtgattcc agacttcgta ttcccaatag
    10681 gatttatgga tgatttagta gcattaaata tagttacaaa attacttaaa aatgataagt
    10741 aagttttaga atcatattta atatataaat aaatcaaagt atataaaatt tattattttt
    10801 tatatgaaac aaattaaaag cacaatgaag tagattaaaa atttaaaact aagaatctaa
    10861 tatatatttt gtaactattt aagtatataa aataaaataa tatccaaact aatacaaaat
    10921 agtaataatt gttttgtact ggaggatata tatttatgaa ttgtaaatgg atatctaaaa
    10981 tagatgagag aaaaaaatgt catagagaag cagattcttc tggatattgt atctttcata
    11041 aagaaaataa gtctgatgaa gaaatacagt tgatgatgga tactattcat aaagaagaga
    11101 taagtgaatt taatggattt gtttttgaaa atgaatttaa tgcggaagaa atattaactt
    11161 acaattataa aatactggat ttttctgaat ccatatttaa acaaaaagca aattttaaaa
    11221 aatatatttt taaaaagaat attattttta actatacaga atttagagat aaagttttat
    11281 ttaatggttg tgtattctta gaaaactgtg attttaatag aagcatattt agtaaaaact
    11341 atataaatga ccgtatattt gaaaaagtga aatttaaagg acctgatttg gtagttaata
    11401 aagtagaaaa ttttccaaga atggatggaa taatttttag catgtgtaca aaatttgtgt
    11461 taaagaatgt tgaatatgga aaaagtgagt atgagcatgg caagataaat tatagaatag
    11521 ctagaaatca agcaacaaaa attggagaat atgagatgat aggattttac tattataagg
    11581 aaagaattta tagtagtaaa ataatgaaac gttctaatta tcctactttc agtgattatt
    11641 tagttgaaaa attttttgac caaatagcac gttacacaac aggttatgga gaaaagccat
    11701 ggaatatatt attagttatt atagcaataa taagtgtttt tgctttatta tacttatttg
    11761 taggcataga aagttcaaat agcacacttg tagctttaga tataaataat ataggtgatt
    11821 actcactaag tgagattttc aaaatgtata tggatttatg gtattttagc atggctacat
    11881 ttagtacagt aggatatgga gatatggttg ctacaagttt gattgggaaa gcattggcag
    11941 gtatagaagt attttttgga gttacaatag gagcaatttg ggcatctgtg attattaaaa
    12001 gaatgataag gtaatatttg tgtaaatatg gataggtatt aattactaga tataaattgg
    12061 actaaatagg gaaaatttaa atataaatat acttaataat ccaagaaggt gaaaaatttg
    12121 aaaaatgcac taaaaaaaat tatatcatta gccatgattc tagtttttat aataccaaat
    12181 tattcatatg cagatgataa atctgtagac aagtattcaa aaacatctat acttatagac
    12241 caagaaacag gtagagtttt atatagtaaa gaacctgata tgaaggtacc attagctagt
    12301 ttaagtaaga tgatgacttt tttacttgca atagaagcta ttgaaaataa agaagtaaaa
    12361 gaaactgata aagtaaaaat agacaaatct attgcatcag taaaagggtc aagttataaa
    12421 cttaaagatg gtgaagaagt gccacttata gagcttatga gaggtcttat gattgtatca
    12481 ggaaatgatg cggcaattgc tatagcaaaa catatttgta aaacgaaaga agcatttgta
    12541 gatagaatga ataaaaaagc aaaagaaata ggaatgagta atacacattt tctaaatcca
    12601 aatggtcttc caatatacga tttaagcaac cccaaaaaac cagcaaaaga aaatatctct
    12661 actgcaaaag atatagctat tcttggaaaa tacatgtttg accattatga aaaacaagta
    12721 acagctataa ctgatatgga gacatataca aattcagata gaagttttga aaaaagcaat
    12781 acaaatgcat tattaagaat tataccagaa gtagatggta taaaaactgg atatactggc
    12841 aatgctggat attgcttatc attttctatg attgtaaata aaaatgataa aaatgagaaa
    12901 aaacgtagag taataggggt tgtattaggt actaaccata aaaataaaag aacctctgct
    12961 tcattagctc tactgaaata tggaaaagaa aatttcaata tgaaaaaagt aattgataaa
    13021 gatagcttta ttggtaagaa atatataaaa ggaatgaaag agttagaggt tacattaaaa
    13081 gcaaaagatg aactttatac aatattaaaa gatgatgaaa gtataaaatc agaagttaag
    13141 attaaaaata tagagtaccc tgttaaaaaa ggtgatacta tgggtattat aaaatattat
    13201 actaattcag gtgacttact tggaagtgta gatatagtaa gtaataatag tatagataac
    13261 gtatcattaa aaacaaaatt aaaaatgaaa tttgcaaatt aaattcaaaa aatggtataa
    13321 aatacacaaa aaaatataaa aatagaaaat atggaaaaaa tattgttatt tcttcacctc
    13381 tgtgatatac tactagaggt gaaaaaataa aataaatcac acacagcatt gggatcctta
    13441 aaaggtgcct attacatggt tttttaggga gaagatcaag ctggaggtat aaaaccagga
    13501 ggtaagatat gtcagtaata tcaatgaaac aattattaga agcaggtgtt cactttggtc
    13561 accaaacaag aagatggaac cctaaaatgg ctaagtatat attcacagag agaaatggaa
    13621 tatatataat cgacttacaa aaaacagtta aaaaagttga agaagcttac aagttcacta
    13681 aagaagtagc tgaaacagga aagccaatct tattcgtagg aactaaaaaa caagctcaag
    13741 atgcgataaa agatgaagct gaaagatgtg gaatgtactt tgttaatgaa agatggttag
    13801 gtggaatgtt aacaaaccac aaaactataa aaacaagaat aaataaatta agagaattag
    13861 aaaaaatgga agaagaaggc gtatttaatg ttcttcctaa aaaagaagtt ataaaattaa
    13921 gagctgaaaa agaaaaacta gaaaaatact taggtggaat aaaagacatg cctgaattac
    13981 caggtgcaat gttcgtagtt gacccaagaa aagaaaacat agctattcaa gaagctcata
    14041 gattaggtat accagtagtt ggtatagttg atactaactg tgacccagaa caattagact
    14101 ttgctatacc aggaaatgat gacgctataa gagccgtaaa attaataact ggtgctatgg
    14161 caacagctgt aattgaaggt agacaaggtg ctgaggaaga agtagctgaa gaccaagaat
    14221 aataatttga caattaaatg gtaagggtcg cccttaccat ttataatatg gaatagaata
    14281 agtaggagga aacttaaaaa atggctaata taactgctca aatggtaaaa gagttaagag
    14341 aaagtacagg cgcaggaatg atggactgca agaaagcttt acaagaagct gaaggaaaca
    14401 tggaaaaagc agtagattta ttaagagaaa aaggattatc aaaagctgct aaaaaagctg
    14461 gtagagttgc tgctgaaggt ctagttgcaa tagaaatgaa tgatgataat acagttgctt
    14521 ctatggtaga agttaactca gagacagatt tcgttgctaa aaacgaagac ttcaaagtgt
    14581 tcgttaaaga tgctgcttgt atggcattag ctactgataa agaagatata gcttctttat
    14641 taggtgaaac tcataaagag ggaataactt tacaagaagt tttaaataat agagttgcaa
    14701 aaataggtga gaaattagac tttagaagat ttgctaaagt tgtaactaat ggacaagttg
    14761 ctggatacat tcacggtggt ggaaaaatag gtgttcttgt tgaaatggaa acagaagcta
    14821 gagatgctaa agttttagaa ttaggtaaag atgtagctat gcaagttgct gctatgaatc
    14881 ctaagtatgt ttcaagagat gaagtagatg ctgaatacat agcacatgaa acagaagtat
    14941 taactcaaca agctttaaat gaaggaaaac cagctaatat agttgaaaaa atggttaaag
    15001 gaagattaga aaaagaatta aaagaggttt gtttattaga gcaaactttc gttaaaaatc
    15061 cagatataac tgttaaacaa ttagttgctg atgttgctaa ggcagtaggt tctgatataa
    15121 aagttgttaa agttgtgaga ttcgaagttg gtgaaggtat acaaaagaga gaagaaaact
    15181 ttgcagaaga agttgctaag caacttaagt aataaccaaa gagagaacac gcaggtgttc
    15241 tcttttttaa aaaatagaaa aaattggata tgaaggattg aaataggagg tttctgatgg
    15301 ataaaccaat gtacaaaagg gtattgttaa aattaagtgg agaagcatta gcaggagaaa
    15361 gaggttttgg tataaataat gatgtagtta atgatattgc tattgctata aaaaaaatac
    15421 aagaaatagg tgttgaagta gctgtagtag taggtggagg aaacttctgg agaggcagaa
    15481 ccagtgaagg aatggacaga actactgctg actatatagg aatgttagct acagttatga
    15541 atgcaatggc gctacaggat gcactagaaa atatagatgt tgctacaaga gttcaaactg
    15601 ctatagacat gagacaaatt gcagaaccat atataagaag aagagcagtt agacacttag
    15661 aaaaggaaag agttgttata tttggtgcag gaacaggaaa cccttacttt acaacagata
    15721 caactgctgc tttacgtgct gctgagatgg aagcagaagt tatattgtta gctaaaaatg
    15781 ttgatgcagt atatgacaag gatccaaaag tacatgcaga tgctaagaaa tttacagaat
    15841 taagttacat ggaagttata cagaaagaat taaaagtaat ggattctaca gctacatctt
    15901 tatgtatgga taataaaatt ccaataaagg tatttgagct tacaacagag aatataataa
    15961 gagcagtaaa aggcgaaaat ataggaacta ctgtaaaata atgaagaatg aaggaggagt
    16021 aaaattatga aactggaaat acataagcaa ttagaagaga aaatgaatgg gacaattgat
    16081 gctttaaagt ttgaatttgg aactataaga gcaggaagag ctaatgcaca aatgctagac
    16141 aaaataagag ttgattatta tggaactcca actccaataa atcaaatagg tgctatatct
    16201 gtaccagaac caagaatatt aatgatatct ccttgggata aatctgctat gcatgaaata
    16261 gaaaaagcga tagctaactc agacttagga ttaaatccat caaatgatgg tgaggttata
    16321 agactttctg tacctgcttt aacagaagaa agaagaaaag aattagctaa aaaagctagt
    16381 aaagctgcag aagaatttaa agttagaata agaaatgaaa gaagagacgc caatgagaaa
    16441 attaaaaaga tggaaaaagg cggagaatta actgaagatg aattaaaaaa agcacaagat
    16501 gaagttcaaa aaatgactga taaatttata aaagaaatag atgcattatt atctaaaaaa
    16561 gaaaaggaca taatggaggt ataatattga aaaaatatta taacctttta ggtcttcatg
    16621 tagatgaggt aaaacaatat tttgatgagc aaaatataaa ttataccgta aaatctattg
    16681 aaggtaaaaa ggataaagaa aaacttgtag ttcctagagt tataaaaata tctgagatag
    16741 gtgatagtgt agaactgata atcacatact tttctgactc cttagtttaa ggagtcagaa
    16801 caatattgga ggcaaaatat gaataacaac attatatatg atatagactt aaataacata
    16861 cctactcata ttgctattat aatggatgga aatggaagat gggcaaaagc tagatttctt
    16921 ccaagaactg caggacataa ggctggagta gaaactataa gagatatagt aaaagaatgc
    16981 tcaaaattag gtgttaagca cttaacatta tatgcatttt caacagaaaa ctggaaaaga
    17041 ccgaaacttg aagttgacac acttatgaac ttattatcta catacttaag aaatgaaata
    17101 gctgaacttc accaaaacaa tgtaaaagta actgctatag gagatataag tgctttacca
    17161 aaaacttgta taagagaatt aaactcagct aaggaactga caaaaaacaa tacaggagtt
    17221 aacttaaatt tagcattaaa ctacggaagt agagcagata taaaaaatgc tcttatagat
    17281 atagtaaaaa attgtgaaag tggtaaaata gatataaaca atattgatga agatattata
    17341 aaaaactatt taagcacaaa gtctatacca gatccagact tggtaataag aacaagtggt
    17401 gaacaaagat tgagtaactt tttactttgg gaagttgctt attctgagtt ttactttact
    17461 gatatacatt ggcctgattt taaaaaagaa gaactacaga aggctatata cgtatatcaa
    17521 agaagggata gaagatttgg aggacttaag taaggagatt attttatgct tacaagaatt
    17581 attgcttcac tagctctggt tccattattt ctatttgttg tatatggagg tattcctcta
    17641 tatatagctg agacagcaat agtttatatt gcattgcatg agttttataa ggcatttaaa
    17701 ataaaagatg tacatccaat attcattata ggatatttat tttctattta tttagctgta
    17761 aaaaatattt ttaatttacc attagaatat acttatgcag taatttttat tttattttta
    17821 gcaagtataa tttatatgtt gatgggcaaa aacaatgtta tagatgtttc gattacgttt
    17881 ttaggagttt tttatatagg agtattttta gattttataa ttataactat aaatggattt
    17941 gaaaaaggta gtatatatgt atggcttata tttgtgatat cttttatgac agatatattt
    18001 gcatatttta gtggttattt acttggaaaa cataagctaa ttcctaaagt tagtcctaaa
    18061 aagactatag aaggagctat tggaggaata ataggaagta cactttgttg tattttattt
    18121 ggatatttat ttggcattga tttgcttcaa ttagcgataa taggaagtat aggaagtgta
    18181 atagctcaat taggagattt atttgcatct tctataaaga gatatgtagg gataaaggat
    18241 tatggaaaga taatacctgg tcatgggggt atattagata gatttgatag tgttatatta
    18301 gttgcacctt ttgtatatag tgcgattaaa ttttttatta gataaagatt ggttggattt
    18361 aaaagagcaa tctataattg ctagtaatga aaatagctat gtaactttta aatgcagaga
    18421 tattctgcag ttattagtta catggctatt ttcattgttc aaaaatttag aagaccttgg
    18481 ggaattgtat aagaataggt attactttat aatagtatac tatatttgta gtgagtatat
    18541 tttttatact gtaactatca ttaaaaaata tttttttgtt taataatttc gatattttta
    18601 gctttattat atatttataa tatatatgta taaatgtaac taatttatta tccatttata
    18661 aattttattt ttaaatataa ttaataagag ataagtaaat tattggtctt attgcacaaa
    18721 aatagatata atttaagtaa tataccaaaa aaaaggaata tatttacata atatgataaa
    18781 ataaattcaa agagttaaat tgtaggtaaa tagtatttta attatttatt aaaatataga
    18841 ttatgtatat gccaaattta aaaagagaca taaagacatt atacaaatat attattgggg
    18901 tgatattttg taagaaaaga agtgatgcag taaattaatt gagtaattta aaaatgattt
    18961 aatgataatg ttgatgaatg tttaaaaaga aattttagaa aaacgtatat tgctgtttgt
    19021 gttggaggtg atgaatttgt agaatttata gggtataaat gattaaacta tttaagataa
    19081 atacatatca ctgtaaaata ttgataatca ttttttataa gagattgatt atttgcaaat
    19141 tatatatatt acaaatgagg taacaataat gaagaaaaaa tatgattttt taatttattc
    19201 tgctatttgg ctagttatta ttattctttt tatattttct ctatatcata gtatagagca
    19261 tattaaaatt ataagtcaca ctggtactat tcgcagtgaa actcaaaagg ttgttaagca
    19321 agaattgaat aatgaaagaa atgatgactt gataaaacga ctggataata ttctgataaa
    19381 gctaagaaca ggtaatggag aaaatggatt tcaaagatgt gataataaag aatttcaaca
    19441 aaaactaaat caaatggatt ctatgtggga atctatgaag aaagaaatta taaaagttag
    19501 aaatggtgct tcaggtgata aattatataa attgagtgaa gagtattctg tgttgtctaa
    19561 tcaaattgtt tttatatctg aaaaacactc taatgcaaaa ttgtattctt ttgctactgc
    19621 actatttatt tatcttatat tctcaacaat aagtctttta atttgggaat attataataa
    19681 aaaaaggttt aaaagaatat tttatactga taatttaaca aaaataaaaa atcaggttgc
    19741 atttgaaaac agagcgattg aaattttgca caatgcatct aacaaagagt atgtactgct
    19801 aaatattgat attgacaatt ttaaatatat taatgataca catggatatg aatatggaga
    19861 taaagttcta ataatagttg caactgttct ttctaaaact tttaatataa aagaaacttg
    19921 tgcaagaatt tgttcagata attttgtaat acttgcaaaa tatagagatt ctcttttaga
    19981 ggatattcgg gaaatgctaa ctaatgctat aatctctgaa ttagatatga atgtaactca
    20041 aactatatct tattgtatag gagcttactt agttgaaatt gataatttag gttataaatc
    20101 tatcaattct atgatggata aggcaaatat tgcacacaag gtaagtaaaa caagaggaat
    20161 ttcctccaca gtatggtata atgaaaatct gttaaaacag ttacaaatgg aaaatagtat
    20221 ttataattat atgtataaag cgatagaaaa tgaagaattt catatgtatt tgcaacctaa
    20281 atttcaaata tcttctttaa atgttgtgag cgcagaagct cttgtaagat ggttttctcc
    20341 agaacttggt tttttatcac cagatgaatt tattccattg tttgaaaaaa gtggatttat
    20401 tattgaattg gacttttata tgttaaaaaa agcgtgttca tttgtaaaaa aaacgtttat
    20461 gaaaaaaaat caatatacat atccgattgc agttaatttt tcaagggtta ctatttacca
    20521 aaatagtttt tatcaaaggt ttttagatac tgttaaagaa tatgaaattc cttttaagta
    20581 tatagagata gaggtaactg aaagtgcttt taatgaaata tctcaaccag ttatttcaat
    20641 tttagaggaa cttaaaaagt taggattttt aatttctatg gatgattttg gatcaggata
    20701 ctcttcatta agtcttttat gttctctatc tattaatgga ttgaagttag ataaaagctt
    20761 acttaaagaa acatttaacc gggagaaagt atatagtatt attcagtgta ttattgaaat
    20821 gtcacatcgt ataggcatgt ctgttgtatg tgaaggtatt gaaactaaaa aagacttgga
    20881 atttttaaat acagttaaat gtgatgtagg acaagggttt tatttttcta aaccgataga
    20941 agaaaaagaa ttttttaaca aatatgtaac taaaaaataa tataattatt tttatcagtc
    21001 tatacattga gtcattttat atctcgttaa atgactcaat gcactgttat gtattttaaa
    21061 atataaatta tttaaaacgt tttttatact tatatagaaa ttcttctata ttttttgatt
    21121 gttctcttat ttcaactgct tctatatcta gtaaataaag tatagaatca tttgtaagtt
    21181 caacatattc accatcttct gaaccgagtg catgtctctc aatttttaat cctttttctt
    21241 cataatattt ttttgtttct atatataatg attctcgctt ttgtatgtag tctttcactt
    21301 cattatttgg aaagttatat gaatccacta atgtttcgcc atttataaaa gttttctcgg
    21361 tagcaacttt ttcatcccaa caatggattt cttcccaagt aaaaaatatc tcagttccat
    21421 ctggaatgaa tgaaaacctg gtaataatat ataaataatt attgaaattt acttcataat
    21481 attcaggaat agcttcaaat tccatatagt aatttgtata attaaagtta ctactatctt
    21541 tttttacaaa tggtattttc tcaataaatt tatatattga ttgttcattt ttaaaaattc
    21601 ctatatcata gcttctttca aattgattaa tttttaataa atatgacata tattctcctt
    21661 atttgttgaa atataatttt tataactcta ctaattttaa acatttttaa ataaatattt
    21721 gtatatacct tatataatat tatagtaatt agttttaaat caatagaata gtatgtattt
    21781 atctaatgcg gcaatataat ttaaagctgg tgttgtagaa aaactaagag tgaatatagc
    21841 aaagagaagc aatagtattt taaactttgt ctctcttatt ttttgaaaac taacattaaa
    21901 acataaggta taaataatat tatcatataa atgaaaacaa atttgaattt ttctctggtc
    21961 gatgtatata gtaagtttgt tgatatgaga aaaattatta ttgataatat acatgcatag
    22021 aaaggaaatg gtatataata ttttcttaag tttctttttt aggcatttct attggcattt
    22081 taggctatag tactaaatac attaaccaac aaaataatga aataaatata tatatagaaa
    22141 acttaatttt cgtgggaaga aaaaaagcaa taatagcaag tattattaga gaataacgaa
    22201 taatacgagt aacatttctt tctttttcta tgatagttaa atcccagtga ttttttataa
    22261 attcaaggtc tttttcttga ctctgattca tttaatcatc tcctgttaat agtataattt
    22321 taaagctatt actttgtttt attttaacat atttatacac taaagtgaat tttatgatta
    22381 gatttattta attatttctg aattaaaact tattagaatt ctctattttg ataatttcaa
    22441 actctattga ttgtatgata attgaaacta taggatatgt aggataacaa gtttcaaagt
    22501 agttaaaata aaaaagataa tgatattatt aatttagaat aaattttata tattatattt
    22561 aattatt
//
LOCUS       DAYAPA010000061        22469 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.061, whole genome shotgun sequence.
ACCESSION   DAYAPA010000061 DAYAPA010000000
VERSION     DAYAPA010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22469
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.061"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            318..1109
                     /gene="pxpA"
                     /locus_tag="ACGI59_002130"
     CDS             318..1109
                     /gene="pxpA"
                     /locus_tag="ACGI59_002130"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HFG9739916.1"
                     /translation="MYKVDLNSDLGESFGTYKIGLDEEVLKYISSANIACGFHAGDPS
                     HMEKTVQLAKKNGVKIGAHPGFLDLIGFGRREMKITKQEAKDYTKYQLGALMAFASSN
                     GCNIQHVKPHGALYNMAAKDKELAMGICEAIYEVDKDIILLGLYNSEMINSAKEIGLR
                     FANEVFADRAYDNNGFLVPRNVEGAVIHDTKHAIDRVVRMVKEGTVETLTGEVIHIKA
                     DSICVHGDNPKAIEFVKEIRKRFELEGIEVCPLENIEVCSSENIV"
     gene            1138..2358
                     /locus_tag="ACGI59_002131"
     CDS             1138..2358
                     /locus_tag="ACGI59_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NRAMP family divalent metal transporter"
                     /protein_id="HFG9739917.1"
                     /translation="MSTKNITDKTKNKKDVGALIGAAFIMATSAIGPGFLTQTAQFTQ
                     DFGPNFSFVILITTILFIGAQVNVWRVIGVSGLRGQDIANKIIPGLGYLVAFLVALGG
                     LAFNIGNVGGAALGMNVMFNMNMTLGTVLSGLIAIFVFMSKNSNSLVDKITKFLALGM
                     IIIVGYVAISNHPPVGEAVSRMVKPENPKGLIFPIITLLGGSVGGYITFAGGHRLIDG
                     GITGEENIKEITKSSLLGILVATMMRVLLFLAILAVVSKGLQLDPENPAASAFKFSAG
                     AIGYKFFGLVLWSAAITSVIGAAYTSVSFLKTLNPFIDKYEKYFIIAFIAISTLIMAF
                     IGKPATLLILAGALNGLILPITLGIMLIASKRKDIVGDYKHPTWLLIFGLIVVLISAY
                     TGITSLSSLGALFA"
     gene            2378..3073
                     /gene="pxpB"
                     /locus_tag="ACGI59_002132"
     CDS             2378..3073
                     /gene="pxpB"
                     /locus_tag="ACGI59_002132"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HFG9739918.1"
                     /translation="METRYLISGDKAVVAEFGNEISEDINKKVISFMRAIEISNLKGI
                     VTEMVPTYRSLMISYNPLEIDFDSLIENLKKIEDNLESIVLPKPKIHEIPVCYDEVFG
                     IDIKNVASYNNLTVDEVIKIHTSREYLIYMLGFTPGFPYLGGMDERIATPRLEVPRTK
                     IYGGSVGIAGSQTGVYPIDSPGGWQIIGRTPLKLYDENREEQILLRAGDFIKFVPITL
                     DEFIEIEKNNLSI"
     gene            3103..4089
                     /locus_tag="ACGI59_002133"
     CDS             3103..4089
                     /locus_tag="ACGI59_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HFG9739919.1"
                     /translation="MGFKVELGGFQTLIQDRGRVGYGQYGVSGCGAMDEYAHRVGNIL
                     VGNSEDEASLEVLMLGPTITFDEYTQIAVTGGDLGAKINGKEIQNWRSYQINPGDVLS
                     FRGVKSGTRAYVSIAGGIDVPLAMGSKSTYTRAKIGGFEGRALKKGDYINTFIQEKDF
                     TINKKLSSKYIPTYSSEIVLRIVKGPQFDAFSNGEVEKFLSNKYKVTNEIDRMGCRLD
                     GESIKHLNGADIISEGISYGAIQVPGHGKPIIMLSDRQTSGGYTKIGNVISVDLYKLA
                     QAKPNDVVKFELVDIYEAHRLLREQEDKIQDIYKSMKNIRVVKAKVLNDIAV"
     gene            complement(4303..4869)
                     /locus_tag="ACGI59_002134"
     CDS             complement(4303..4869)
                     /locus_tag="ACGI59_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFG9739920.1"
                     /translation="MLGEKMRNIRKSKKKTLSDVSKLTDLSISYISQIERDAIEPSLS
                     SLRKIAEVLDTPLYMFMDDNNTDDLVIRKEDRVMMKFPKSEMFYEIVSPMPTTEFTPS
                     ILFVEFELKPESEDAKDYIYHASEEIMVVTSGVVDICMGEKVVRLNPGDSTFIKANVP
                     HKIINPSKDTIARGYGVISPPIWPIKSK"
     gene            5221..6288
                     /locus_tag="ACGI59_002135"
     CDS             5221..6288
                     /locus_tag="ACGI59_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HFG9739921.1"
                     /translation="MKITDIKFEKLRIKLKKPVVVSFGVIEYGESIILKIETDEGYCG
                     FGEAAPLAAVTGEVLDNVLSVLLMFKKELIGKDPLDIETIHTIMDGIIIGNTSAKAAI
                     DIALYDIKGKIMNAPLYKVLGGFDSKVQTDITISIDKPEKMAVEALERVKEGFRILKL
                     KAGINPEDDIEAVKLIREAVGESIRIRIDANQGWNVNSSINTIKKLEEFNVDAIEQAL
                     PHWDLDGTAYIRNKSNTKIMIDESLHSPIDAIKAIKKNAVDTFNIKLMKSSGIYPAIK
                     INNIAEASGVNCMLGCMLETRIGITAAANLIASKKNITEADLDSFMFCEELEGISGGF
                     VMDGDIMNLVNKPGLGIEVNL"
     gene            6307..7467
                     /locus_tag="ACGI59_002136"
     CDS             6307..7467
                     /locus_tag="ACGI59_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HFG9739922.1"
                     /translation="MNTLFSNPGSILAVMTSMIALGFYLQRYKAIKSLGPALTIIIMG
                     IILSNLKVVPVSTELYGTISTYAIPVSMTIMLMSVDLKEMTKLSREPLIAIFVAVLTV
                     SIMAFLFGLVFAEKISEGWKVAGMFVGTYTGGSANLTAIGTGLNVSRQTLAAANAADY
                     VIGVPTLIFMFALPAILKNSKKFKKLWPYHVEESELEDCQNEEFMESKEWSIKDIAWM
                     LAIGFVVTEVATILAGYFNSSFSSAARILLVTTISIIIAQLKPVKKLKGNLDLGLFVA
                     LFFLCTIGFSVDIKEFLGSTFTITLYCFSIIFASFVFHLGITRLLKIKYQYVILSIVG
                     AIADGPTSALVAASAKWNSLVSVAVVMGVIGGVLGNYAGISVAYAIKMSLGL"
     gene            7480..9576
                     /locus_tag="ACGI59_002137"
     CDS             7480..9576
                     /locus_tag="ACGI59_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HFG9739923.1"
                     /translation="MIKFKLYISGLYSGNVIFDGDLLIEKLNPFTNKIESLKPISKEE
                     NTYYLNLTKIDLKSLFNNFDVYTKSLVNTDKDTVINNLGETHSKLNEYIWVQRNKKFP
                     LDIIIVDNKIVGFICLSRETCTILIMDGYEEYTVLKEWEKTHKNEEIYSIRFGGNYMI
                     DMKDGIKLSTDVYLPDFVDSTKKAPTILMRTPYGKENDKEIYYKYVQRGYAVVIQDVR
                     GRNESEGKWEPMIHEREDGDSTINWIVSQEWSSGIVGMLGASYLGYVQWAAASSGNKH
                     LKALVSIVTSGSPFIDIPRKGGAFVSGMLAWAFMVSRNKVDRSKMVRDDWDDVLNIRP
                     IENIPVEALGYRIEFLEEWLKRVEKDEYWDLMDWHLQKDKINVPALVVSGWYDDNSMG
                     TTEALDVIKDYEKGKRKAILGPWMHNSNTLRDINGISLGNSSLRYDLDYNYLLWFDKY
                     LKGIENNIDTTAPVEYYSVGSNKWKTEENWPIINKIDKSMYLISDGNANTSLGNGRLV
                     FDNGLEEKYDSYIYNPKDPSVQLIDMSENEVGVPNNYKDLEKRSDMLCYTSDAFSEEF
                     TVTGDIKLEFFASSSAKDTDWVIKIMDVDLDGNSIKLADGILSARFRNSFYKSEFMEE
                     GEIYKFTIITSKISNTFKVGHKIRLDITSSAKNFIFQNSNTTEGYNSIEYIEAKNTIY
                     HGGKYPSKLILPIENK"
     gene            complement(9735..10079)
                     /locus_tag="ACGI59_002138"
     CDS             complement(9735..10079)
                     /locus_tag="ACGI59_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFG9739924.1"
                     /translation="MEIKRYEGTGRMSRAVVHNNTVYLCGQTHAEGGVIEQTTEVLAK
                     IEDLLNKYGSDKQHLLSVTIYLRDMKDFEAMNSVWDAWVEKGFEPARACVEARLAREH
                     LLVEMSVVAATK"
     gene            10371..11261
                     /locus_tag="ACGI59_002139"
     CDS             10371..11261
                     /locus_tag="ACGI59_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFG9739925.1"
                     /translation="MSSTIKSNRIKGIMFIIASALGFAMMSAFVKLAGDLPSFQKVFF
                     RNLVSAIIAFWLIIKHKGSLTGKKENLKVLLYRSIFGTLGVIFNYYAIDKLMLSDANM
                     LNKMSPFLVVIFCAIALKEKINVKQIGAIIIAFIGALFIIKPTFSVEIIPYLGGIAGA
                     VFAAMAYTCLRVLGDREDYYTIVFFFSVFSLVTVGPIAFAVYEPMTLMQLIYLLLGGV
                     FASLGQFGITLAYKYAPAKEISIFDYSNIIFSAILSIFLFNVYPDMLSVVGYLIVFSA
                     AFYMFLYNKKLDKLDKEKKK"
     gene            11479..12765
                     /locus_tag="ACGI59_002140"
     CDS             11479..12765
                     /locus_tag="ACGI59_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFG9739926.1"
                     /translation="MEKVKVAVIGAGNRGTYAYAPYIYENSDVCEIVAVAEPKKGRRE
                     LFTQKYNLDSKNVFETLEDFFKHDKMADAVIIATNDDRHYDVAKLALEKGYHVLLEKP
                     MSNSLDGLVHIDDLCDKYKDKIFMICHVLRYSPFYNKLKEIVESKKLGELVSIQYNEN
                     IGYWHFAHSFTRGNWRNSNETSPLILAKSCHDMDILLYLVGSRCKKISAFGSLKHLNN
                     QNASGEMAQNCLQCLVEKKCPYSAKRIYLEKDRSINRAVHINPTEENLLNILKTSPYG
                     RCVYRCDNNVVDNMVNILQFENGVTATFNLCAFTKENGRTIKLMFSHGEVGGDLNKNE
                     IRIKEFGKNEEIVMNPSNQQNMVEEYDRNLIAEFIKLVSNKELEKGRVAAKEAIQSHV
                     MAFAAEYSRVSDEVVYIEEFFDSAKQMTKEIEETIF"
     gene            complement(12854..13297)
                     /locus_tag="ACGI59_002141"
     CDS             complement(12854..13297)
                     /locus_tag="ACGI59_002141"
                     /inference="COORDINATES: protein motif:HMM:NF025547.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory YrvL family protein"
                     /protein_id="HFG9739927.1"
                     /translation="MIKISNKRRIPLGEFISVFLFFAVFFGITFCIFTVIGIGFLSFL
                     GFEYKSLGAVLIFFLIYFCITTPIDFLCTTILDIFRYVNKLPYSIYKLCEFIIDFILT
                     FLAMNIIDTFMDSVTIPLSTEILFALLSYLLSECMDFFDRDKKKP"
     gene            complement(13321..14115)
                     /locus_tag="ACGI59_002142"
     CDS             complement(13321..14115)
                     /locus_tag="ACGI59_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HFG9739928.1"
                     /translation="MKLYLSADIEGTCGIVNWDETKLECEFSQHYRTQMSKEITAACD
                     GATQSGVHEILVKDAHDSGRNINPNILPENVKILRGWTKDPLVMMAGIDESFDACIFT
                     GYHSGATCNGNPLSHTMDIDYDYFKINDEIASEFTINAYIAAYYNVPVAFLSGDEMLC
                     ESAKKLNPNIVTVPVSKGIGNGSISIHPNLALKKIEKGVKKALSGDLSRHLIKLPDKF
                     KIEIKFREHYKAFKASFYPNMKKIDSQTVLFETEDYYEFLRMLLFI"
     gene            complement(14680..15156)
                     /locus_tag="ACGI59_002143"
     CDS             complement(14680..15156)
                     /locus_tag="ACGI59_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HFG9739929.1"
                     /translation="MKEILINPIGKICIQGEEVFIKLDKKYVPALNELDDFSHLNVFW
                     WADGFDNPELRSILETPKPYKSGPDVIGIFATRSPIRPNPIALTAVQIINIDHENGII
                     KIPYIDANDNSPVIDLKPYTPSVDRVENPSIPKWCSHWPKSVEKSGEFNWEDEIEF"
     gene            complement(15257..16426)
                     /locus_tag="ACGI59_002144"
     CDS             complement(15257..16426)
                     /locus_tag="ACGI59_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFG9739930.1"
                     /translation="MLNIKTLCHEINDWVINIRRDLHKTPELGLEEFQTKKKIIKYLN
                     EIGINYIEYKNHTGITAYINVSPNFETVAIRADIDALPITEELNYSYKSINIGKMHAC
                     GHDAHTAILLGTCNILFKLKDYLNVNVKFFFQPAEETIGGAQLMIEDGCLENPNVKYI
                     FGLHVNSNINKNLIELKYNTLNASTDTLQLTVHGSKCHGAYPHQGIDAIVISAHIITA
                     LQTIVSRNTNPTDSVVISLGKIEGGIKENIVCDKVVIRGTLRTLTPETREFSKKRIRE
                     ICDFTCKTFGGSISVEIEEGYPALINSNHLVDYVKQNAVELFGEENIILKDSPTLGAE
                     DFSYFLRHCEGAFYHLGCANREKNITSPLHTSTFDIDEDCLITGVMLHVKNVLSF"
     gene            16589..17452
                     /locus_tag="ACGI59_002145"
     CDS             16589..17452
                     /locus_tag="ACGI59_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HFG9739931.1"
                     /translation="MLGIIAPSGPLRNTSLEEIKFNLESYGYEVKFSESCSLNYKGYL
                     AGNDDIRARDIEDMFLDKDVDIIMCLRGGYGTTRILDKINYDIIKQNPKPFIGFSDIT
                     GLNLAFYKNCGLLPYHGIMAANVGKWDKFTYKSLVNALEFKDELYLENPKEEKIYTVC
                     EGKAEGIIMGGNLSLIIATMGTKYEIDAKDKILFIEEIGEPQYKLDRMLTQLYSSGKL
                     EECNGIIFGDFKDCIEENDLMELLIEFANKVNKPSIYNLQSGHCIPMITIPLGRMCEL
                     DATEKIVKLKK"
     gene            17523..18116
                     /locus_tag="ACGI59_002146"
     CDS             17523..18116
                     /locus_tag="ACGI59_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3867 domain-containing protein"
                     /protein_id="HFG9739932.1"
                     /translation="MSDDRIIDFNELKNKVKDSDVDKFEQYIYNLYFSVMDGKMSMAE
                     FSRKIFDYMRDNNISQEKFMKIQKQFMERYGMDTEEVEKQLRNFGIDPSTAGFMSNNT
                     SSKVSTEDLESFKKSAGFYEKYGEKIQPKSCITTFIKNDLNDINVIIDQEKIMLCSDR
                     KINLMDSELNEFLLEYKNMFNKKIKVVMCETTNKYDY"
     gene            complement(18367..19002)
                     /locus_tag="ACGI59_002147"
     CDS             complement(18367..19002)
                     /locus_tag="ACGI59_002147"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HFG9739933.1"
                     /translation="MDNLLNELEALKSNLDNLPIEKIENYDLSKTALFIIDVNNGFAR
                     QGALYSPRVESLIKPIEMFTKKISNKLNRVIAFTDSHTPKSIELLSYPVHCLENDVES
                     ELVDELKSIENLQILPKNSTNGFFALENLDFDNIDNIIIVGDCTDICIYQFAITLKSY
                     FNQHNIEKNIVVPMNLVDTYDIPNVHPAEILNLVFFNSMIQNGVNVLKEIR"
     gene            complement(19082..19813)
                     /locus_tag="ACGI59_002148"
     CDS             complement(19082..19813)
                     /locus_tag="ACGI59_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFG9739934.1"
                     /translation="MNIYAHRGFSGKYPENTILAFKKCLDMDIYGIELDVHRTKDGKI
                     VVIHDEKVDRTFNGHGFVKDFTLRKLKTLNSSFEGYQSNKECKIPTLEEVLILISPTD
                     LTLNIELKTDKINYPNIEKGVLELILKYNMKNRVLISSFNSNSLKNFHKLDPSVKTGL
                     LCYLPINNVVNFAKFLGNSYLHPPLVLVNESLIELCHKNLLGVNVYTVNEEDDILHCL
                     KLNVDGIFTNYPDIASNLLHSKQYS"
     gene            20144..22333
                     /locus_tag="ACGI59_002149"
     CDS             20144..22333
                     /locus_tag="ACGI59_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HFG9739935.1"
                     /translation="MYEVNLERAMNYWMDLLDDYTNIAKIPNDFFYKDCSDEVVDEVF
                     KMNIEKHERLIQYSKVNNISIDSIIETIYGLILQKYIYEDDVVFGKTMNIIPIRVRVR
                     QSEKFIDVVRKLKRQWSRSLEYGYYLLSDIENKNHLNTKLINTIFIAKSFDLSYDYDI
                     EKKGYDLSVIVYTKRALKIKIKYKPSIYKRETIRRILNQFDYIIEQIIEDNDILIEDL
                     EFVCKEEEKVLLEDFNNNQYEVPYDKSYVDLFREQVKVTPSNIAARDENRKLTYKELD
                     EVTDKLAGYLNHIGVESEDIVAVMLPRDINIIITAIGIMKSGGAFFPIDTSNPEERLN
                     YLLEDSNAKVVITTDELKSKVVNENTIVLDINDEEMFKFGYELTEKITSSNCAYTIST
                     SGSTGRPKTIAIEHKSLVNMCYYSVKSISATENDICGIYLSFSFDAVMKQLFPYLLVG
                     ASIDIMPEEAKFDEYTVNEYCEENDITILALPTAFARLFIQNCNNNSLRVVQTGGERL
                     KGYRKRNYELYNEYGPTEFTVVSTSFHVDREYGKIPIGKPIFNTYAYVLDKKNKLCPI
                     GAPGELCLSGIQISRGYLNKKGLTEQVFVENPYKTCEHNKLMYRTGDLVRWLDDGNLD
                     YIGRMDNQVKIDEFRIELYEIENIINNITEIKSVVCISRTNDDGDIYICAYYVIDEED
                     SGKINERTIREYLNEHLPPYMIPTIIMRIDKIPVTPIGKVNKRALPE"
BASE COUNT         8622 a         2570 c         3773 g         7504 t
ORIGIN      
        1 gcatataaag tgattaaaaa tgaaaaatac tttaaaaatg aaggttaaag cccttatttt
       61 aagtgaaaat aattaattat taactaaaaa atggtaaaaa gattttttga aaaagaaata
      121 caaatgaatg ttcaattaaa ataattgcaa aaatattcct taaatggtaa aattatatta
      181 attaaatgca atagaatagt tgcaaaaata aaattgaata caagtgataa aatatttcat
      241 gcaatatata aaataaacaa aatttaaaaa ttaaaactac ttattctatc aaaatgttag
      301 cgaggagggg tcataaattg tataaagtag atttaaatag tgatttagga gaaagttttg
      361 gaacgtataa gattggttta gatgaagaag ttttaaaata tatttcatca gccaatatag
      421 cttgtgggtt tcatgcaggt gaccctagtc atatggaaaa aacagtacag ctagctaaaa
      481 aaaatggagt gaaaataggc gcacatccag gtttcctaga tttaatagga tttggaagac
      541 gtgagatgaa aataacaaag caggaagcta aagattatac aaaatatcaa ctgggtgctt
      601 taatggcatt tgcaagttca aatggatgta atatacaaca tgtaaaacca catggagcat
      661 tatacaatat ggcagctaaa gataaagagt tagctatggg gatttgtgaa gctatatatg
      721 aagtcgataa agatattata ctgttggggc tatataatag tgaaatgata aattctgcta
      781 aagaaattgg acttaggttt gcaaatgaag tatttgcaga tagagcttat gataataatg
      841 gttttttggt tccaagaaat gtagaaggag cagttataca tgatacaaaa catgcaatag
      901 atagggttgt taggatggta aaagaaggaa ctgtagaaac tttaacaggt gaagttatac
      961 atatcaaagc agattctata tgtgtacatg gggacaatcc aaaagcaata gagtttgtca
     1021 aagaaataag aaagcgtttt gaattagaag gtatagaagt ttgtccatta gaaaacatag
     1081 aagtttgttc gtcagagaac atagtataag actgataaca tgagggggag ataaaatatg
     1141 agtacaaaaa atattacaga taaaactaaa aataaaaaag atgtcggagc acttataggg
     1201 gctgcattta taatggcaac atcagcaatt ggaccagggt ttttaactca gactgcacaa
     1261 tttacccaag attttggacc gaatttttca tttgtaattt taattacaac aatccttttt
     1321 ataggggctc aagtaaatgt atggagagta attggtgtat caggacttag aggtcaggat
     1381 atagcaaata aaataatacc tggattaggc tatttagtag cttttttagt agctcttgga
     1441 ggattagctt ttaatatagg aaatgttggt ggagctgcac ttggtatgaa tgtaatgttt
     1501 aatatgaata tgacattagg aactgtatta agtggattaa tagctatatt tgtatttatg
     1561 tcaaaaaatt caaattctct agtcgataag ataactaaat ttttagcatt aggaatgata
     1621 attatagttg gatatgtagc aatatcaaat catcctcctg ttggagaggc agtatcaaga
     1681 atggtaaaac cagaaaatcc aaaaggactg atattcccaa taataacttt acttggagga
     1741 agtgttggag gttatataac ttttgctgga ggacatagat taatagacgg tggaataaca
     1801 ggagaagaaa atataaaaga aataactaaa tcttctcttt tgggtatatt agttgccaca
     1861 atgatgagag ttcttttatt tttagcaata ctggcagtgg tttcaaaagg tcttcaactt
     1921 gacccagaaa atccagcagc ttcagctttt aaatttagtg caggagcaat aggatataaa
     1981 ttctttggac ttgtattatg gtctgctgca attacatcag ttataggagc tgcttataca
     2041 tcagtttctt tcttaaagac actaaatcct tttatagata aatatgaaaa atattttata
     2101 atagcattta tagcaatatc aacacttata atggcattta taggaaaacc agctacttta
     2161 ttaattttag caggagcttt aaatggattg atacttccaa tcacattagg aattatgctt
     2221 atagcatcta aaagaaaaga tattgttgga gattacaagc atcctacatg gcttttaata
     2281 tttggattaa tagtagtttt aatatctgca tatacaggaa taacttctct tagcagccta
     2341 ggtgctttat ttgcttaatt tagaagaggt gacatttatg gaaactagat acttgatatc
     2401 aggggataag gcagtggttg ctgaatttgg aaatgagata tcagaagata taaacaaaaa
     2461 agtaatttca tttatgagag caatagaaat atcgaatctt aaaggtattg taacagaaat
     2521 ggtaccaaca tataggtcac ttatgataag ttataatcca ttagaaatag attttgatag
     2581 tttgattgag aatttaaaaa aaatagaaga taatttggaa agtatagtat taccaaaacc
     2641 taaaatacat gagattccag tatgttatga tgaggttttt ggtatagata ttaaaaacgt
     2701 tgcaagctac aataatctca ctgtagatga agttataaaa atacacacaa gcagagagta
     2761 cttgatatat atgctagggt ttactcctgg atttccatat ctaggtggta tggatgaaag
     2821 aatagctaca cctagattag aagtgccaag aactaaaatt tatggtggga gtgttggaat
     2881 agcaggttcc cagactggtg tatatcctat tgatagtcct ggtggatggc aaataattgg
     2941 taggacacct ttaaaattat atgatgaaaa tagagaagaa cagattttgc ttagagctgg
     3001 agactttata aaatttgttc caataacatt agatgaattt atagaaatcg aaaaaaataa
     3061 cttaagtatt taaatatcgt tatagtacag ggagtggtat atatgggatt caaagttgaa
     3121 ttaggaggtt tccaaacatt aatacaagac agaggtagag ttggctatgg tcaatatggt
     3181 gtatctggat gtggagcaat ggatgagtat gcacatagag taggaaatat attagtagga
     3241 aattctgaag atgaggcttc tttagaagtt ttaatgttag gtcctacaat tacctttgat
     3301 gaatatacgc aaatagcagt aactggggga gatttagggg ctaaaataaa tggaaaagaa
     3361 attcaaaact ggaggtcata tcaaataaat ccaggtgatg tattatcatt tagaggtgtg
     3421 aaatcaggta ctagagccta tgtgtcaata gctggtggaa tagatgttcc acttgcaatg
     3481 ggaagtaagt caacttatac aagagctaaa attggtggat ttgaaggtag agcacttaaa
     3541 aaaggcgatt atataaatac ttttattcag gaaaaagatt ttacaataaa taaaaaatta
     3601 agttcaaaat atattccaac ttatagttca gaaatcgtac taagaattgt aaaaggtcct
     3661 caatttgatg ctttttctaa cggtgaagta gagaaatttt tatcaaataa atacaaagtt
     3721 acgaatgaaa tagatagaat gggttgtaga ctagatggag agagtataaa gcaccttaat
     3781 ggagcagata ttatatctga gggtatttct tatggagcta tacaagtacc tggtcatgga
     3841 aaacctatta taatgttatc agatagacag actagtggtg gctatactaa gatagggaac
     3901 gttataagtg tagatttgta taagttagca caagcaaagc caaatgatgt agtaaaattt
     3961 gaacttgtag atatatatga agcacataga ctattgagag agcaagaaga taagattcaa
     4021 gatatctaca agagtatgaa aaatataaga gttgtaaagg caaaagtatt aaatgatata
     4081 gcagtttaat tttaacaaat acaattttta ttatagttta taatataact tacaaatagc
     4141 tatttactat ttaccataat taagatggtt tagattagtc aaatagctat ttttttagtt
     4201 tgagtaaata ttttaaattt aaataaatac ttcaatttta aaataaaagc aatatttaaa
     4261 tctattatat tatctacaaa aagtaggtgt gtcatattga gtttatttag attttattgg
     4321 ccaaattgga ggtgatataa ctccatatcc acgagctatt gtatctttac ttggattgat
     4381 gattttatgt ggtacattag cttttataaa tgttgagtca cctggattta atctaactac
     4441 cttttcaccc atacaaatat caaccacacc actcgttacc accataattt cttctgatgc
     4501 atgatatata taatctttgg catcctcact ttctggttta agttcaaact ctacaaataa
     4561 aattgaaggt gtaaactcag ttgtaggcat tggagatact atctcgtaaa acatttcact
     4621 ctttggaaat ttcatcataa ctctatcttc ttttctaatg actaaatcat cagtattatt
     4681 gtcatccata aacatatata aaggagtgtc taaaacttca gcgatttttc tcaatgaaga
     4741 caaggatggt tcaatagcat ctctttctat ttgagagata taacttatag ataaatctgt
     4801 caatttggaa acatcactta atgttttttt cttgcttttt cttatatttc tcatcttttc
     4861 acctagcata agaacctcca aataataaaa ttaatataaa atattcgctt aaaacaaagt
     4921 aattatagca caacatagaa gtagtggcaa taagaataca tagtagattt tgacaattaa
     4981 aatttaatta aatccaaata aaagtctatg aaaatgaatt attgttaaat taatacaaag
     5041 aaaataataa taaaaaaatt taatagaaga gtattaattg aattaagatt atcacattat
     5101 attttggaaa agagtaaatt ttcagtaaat tatcttgaca tatatattga agtaataata
     5161 taatttaaag tataaaattt taaaaattga attataactt caaaaaataa ggagggtaat
     5221 atgaagataa cagatatcaa atttgaaaag cttagaatta aattaaaaaa acctgtagta
     5281 gtttcatttg gagtaattga atatggagaa agtataattt taaaaattga aactgatgaa
     5341 gggtattgtg gatttggcga agcagcgcct cttgcagcag ttacaggtga agttttggat
     5401 aatgttcttt cagtattgct tatgtttaaa aaagagttaa taggcaaaga cccactagat
     5461 attgaaacca tacatacaat catggatgga attattatag gtaatacttc tgcaaaagca
     5521 gctattgaca tagctttata tgatattaaa ggaaaaatta tgaatgcacc cctttataaa
     5581 gttcttggag gatttgatag taaagtccaa acggatatta ctataagtat agataagcca
     5641 gaaaaaatgg cagtagaagc tttagaaaga gtaaaagaag gttttaggat attgaagctt
     5701 aaagcaggta taaatcctga agacgatatt gaagctgtaa agttaattag agaagccgtt
     5761 ggagagagta taagaattag aattgatgca aatcaaggat ggaacgtaaa tagcagtata
     5821 aatactatca aaaagttaga agaatttaat gttgatgcaa ttgaacaagc acttccacat
     5881 tgggatttag atggtactgc ttacataaga aataaaagta atacaaaaat tatgatagat
     5941 gaatcattac attcaccaat agatgcaatt aaggctataa aaaagaatgc agttgataca
     6001 tttaatatta agctgatgaa atcaagtgga atatatcctg ctataaaaat caacaacata
     6061 gctgaggcaa gtggagtaaa ttgtatgtta ggatgtatgc tggaaacgag aattggtata
     6121 acagcagcag caaatttaat agcatctaaa aagaacataa cagaagctga ccttgatagt
     6181 tttatgtttt gtgaagaatt agaaggtata tctggaggat ttgtaatgga tggagatatt
     6241 atgaatcttg tcaataaacc aggtttagga atagaagtaa acttatgata aatttggagg
     6301 ttaaatatga atacactttt ttcaaatcca ggtagtatac ttgctgtaat gacatcaatg
     6361 attgcattgg gtttttactt acaaagatac aaagccatta aaagcttagg accagcactt
     6421 acaataatca taatgggaat aatactttca aacttaaaag tagttccagt aagtactgag
     6481 ttatatggaa ctatttcaac atatgctatt ccagtttcta tgactataat gttgatgagt
     6541 gttgacttaa aagaaatgac aaaattatca agagaacctt taattgcaat ttttgtagct
     6601 gtattaactg taagtatcat ggcattttta tttggattag tatttgcaga aaaaatatca
     6661 gaaggttgga aggttgctgg aatgtttgta ggtacatata ctggtggaag tgctaatctt
     6721 acagctattg gaacaggtct taatgtaagt agacaaactc ttgctgcagc aaatgcagca
     6781 gactatgtaa ttggagtacc aacattgata tttatgtttg ctctaccagc aatattaaaa
     6841 aattcaaaga agtttaaaaa actttggcca tatcatgtag aagaatcaga attagaggat
     6901 tgtcaaaatg aagaatttat ggagtcaaaa gaatggagta ttaaagatat agcttggatg
     6961 ttggctatag gttttgttgt tacagaagtt gcaacaatac ttgctggata tttcaattcg
     7021 agttttagta gtgcagcaag aatactttta gttacaacaa tttcaattat aattgctcaa
     7081 ttaaagccag taaagaaact aaagggaaat ttagatttag gattatttgt tgcattattc
     7141 tttttatgta caataggttt ttcagtagat ataaaagaat ttttaggttc aacttttact
     7201 ataacacttt actgttttag cataattttt gcttcatttg ttttccattt aggcataact
     7261 agacttttaa agataaagta tcaatatgta atactttcta tagtaggagc aattgctgat
     7321 ggtcctactt cagctttagt agctgcttct gcaaagtgga attcgcttgt aagtgtagca
     7381 gtcgtaatgg gagtaattgg tggagtatta ggaaattatg caggtatatc agtggcatat
     7441 gcaataaaga tgtctctagg attatagggg ggagtttaat tgataaaatt taaattatac
     7501 atatctggac tatacagtgg aaatgtaatc tttgatggag atttattaat tgaaaagttg
     7561 aatcctttta caaataaaat agaaagttta aaaccaataa gcaaggaaga aaatacatac
     7621 tatttaaatc ttacaaaaat agatttaaag tctttattta ataattttga tgtatatact
     7681 aaaagcttag taaacacaga taaagacaca gttataaata acttaggaga aacgcattct
     7741 aaacttaatg aatatatatg ggttcaaaga aacaaaaagt tcccactaga tataattatt
     7801 gtagataata aaatagtagg ttttatctgt ttatccagag aaacttgtac tatattaatt
     7861 atggatggat atgaagagta tactgtatta aaagaatggg aaaaaactca taaaaatgaa
     7921 gagatatatt ctataagatt tggtggaaat tatatgattg atatgaaaga tggtataaaa
     7981 ctttctacag atgtatatct ccctgatttt gtagattcta ccaaaaaagc accgacaatt
     8041 cttatgagaa ctccatatgg aaaagaaaat gataaagaaa tatattataa atatgtacaa
     8101 agaggatatg ctgttgtaat acaagatgtt aggggaagaa atgaatcaga aggtaagtgg
     8161 gaacctatga ttcatgagag ggaagatgga gatagtacca taaattggat agtatcacag
     8221 gaatggtcaa gtggaatagt tggaatgctt ggagcatcat atcttggtta tgtccaatgg
     8281 gcagctgcat cttcaggcaa taagcatctt aaagctttag ttagtatagt aacatcagga
     8341 agccctttta ttgatatacc aagaaaagga ggagcttttg tatctggtat gcttgcatgg
     8401 gcatttatgg tatcaagaaa taaagttgat agaagcaaaa tggtaagaga tgattgggat
     8461 gatgttttaa acatacgacc aatagaaaat atacctgttg aagctcttgg atatagaatt
     8521 gaatttctgg aagaatggtt aaagagagta gaaaaagatg agtattggga tttgatggat
     8581 tggcatttac aaaaagataa aataaatgta cctgcattgg ttgtatctgg gtggtatgat
     8641 gataatagta tgggaactac agaggcctta gatgttataa aggactatga aaaaggaaag
     8701 agaaaggcta ttctaggtcc atggatgcat aattcaaata ctttaagaga tataaatggt
     8761 atttctttag gaaatagttc attaagatat gatttggatt ataattatct attatggttt
     8821 gataaatact taaaaggtat agaaaataac atagatacga ctgctccagt agagtattac
     8881 tctgtaggtt ctaataagtg gaagacagaa gaaaattggc caataataaa taagatagat
     8941 aaaagtatgt atctaataag tgatggaaat gctaacacat ctttaggaaa tggtagatta
     9001 gtatttgata atggtttaga agaaaagtat gatagttata tatataatcc aaaagaccca
     9061 tcagtacaac taattgatat gtcagagaat gaagttggag ttccaaataa ctacaaagat
     9121 ttagaaaaaa gaagtgatat gttgtgttat acatcagatg cttttagtga agaatttact
     9181 gtaacaggtg atataaagtt agagtttttt gcatcaagtt ctgccaaaga tactgactgg
     9241 gttataaaaa ttatggacgt ggatttagat ggaaattcaa ttaaattagc tgatggtata
     9301 cttagtgcta gatttagaaa tagtttttat aagtcagagt ttatggaaga aggagagatt
     9361 tataagttta cgattataac ttcaaaaata tcaaacacgt ttaaagttgg tcataaaatt
     9421 agactagata taacgtctag tgctaaaaac tttattttcc aaaatagcaa cacgacagag
     9481 ggatataata gtattgaata tatagaagca aagaatacga tttatcatgg aggtaaatat
     9541 ccatctaaat tgatattgcc aatagagaat aagtaaattt atatagatta aatagtgagg
     9601 aggtgtctca aaatgaattt tttagttcgt gagacacttt cttttataca aaatcaaatg
     9661 tattttcatc atttcaacaa tgaaaaagag gcttatctct ctgttttgag atagcctcct
     9721 ataagctaaa ttgattattt tgtagctgca acaacgctca tttcaactaa caaatgctct
     9781 ctagcaagtc tagcttcaac acaagctcta gcaggttcaa atcctttttc aacccaagca
     9841 tcccaaacag agttcatagc ttcaaagtct ttcatatctc ttaaataaat agtaacagat
     9901 agcaaatgtt gcttatctga accatatttg tttaataaat cttctatttt agctaaaact
     9961 tcagttgttt gttcaattac tcctccttca gcatgagttt gtccacataa ataaactgta
    10021 ttgttatgaa ctacagctct actcattctt ccagttcctt catatctctt tatttccata
    10081 ttttaaatac ctccatagtt aaaaaatcta ttaaaaatct aaataataaa ttaaaaaaag
    10141 tctatactat ataaaattat attatatatt ctgataataa tccataaaaa catataactt
    10201 tttagctata attattattg ttattaattg aaaagattta ttagttactt gacatagttt
    10261 acacttttaa ttatagtata aattggaata tttagataat tattatttaa tgtataagct
    10321 aagtttttaa ttaatatggt tttatacatt tatatagaaa agaggtacgt atgagtagca
    10381 caatcaaatc aaatcgaata aaaggtataa tgtttataat agcatcagcg cttggctttg
    10441 caatgatgtc tgcattcgta aagctagctg gagatttacc aagttttcaa aaagtgttct
    10501 ttagaaatct ggtatcagct ataatagctt tttggcttat cataaaacat aaaggaagtc
    10561 taactggtaa aaaagaaaat ttaaaagtac ttttatatag gtcaatattt ggaactctag
    10621 gagttatatt taactactat gcaattgaca agttgatgct ttcagatgct aatatgctaa
    10681 ataaaatgag tcccttcctt gtagtaatat tttgtgcaat tgctctaaaa gaaaaaataa
    10741 atgtaaagca gataggagct attataattg catttatagg agctttattc ataataaaac
    10801 caacatttag tgtagaaata attccatatc ttgggggaat tgcaggagct gtatttgcag
    10861 caatggcata tacttgttta agagttcttg gagatagaga agattactat acaattgtat
    10921 ttttcttttc agttttttca ttagttactg tagggccaat tgcttttgca gtatatgaac
    10981 caatgaccct tatgcaactt atatatctat tactaggggg agtatttgca agtctaggtc
    11041 aatttggaat aactctagca tataaatatg cacctgctaa ggaaatctct atatttgact
    11101 atagtaatat aatcttttca gcaattttaa gtatattctt attcaatgtc tatccagata
    11161 tgttaagtgt tgttggatat ttaatagtat tttcggcagc attttatatg tttttataca
    11221 ataagaagtt agataaatta gacaaagaaa agaagaagta aactagataa aagtgttttt
    11281 ataatttgta ttgaatattt aagtctttgt attaaaatta aagaaaattt aattaaaaat
    11341 gtttggtata ttaacttata acagcagtta aaattgaaaa attataactg ctgtttcttt
    11401 tattaaaaat atccaaatgt ctgtaaaaat agactataat agagtagtat aaataatata
    11461 aaggggataa attaaagtat ggaaaaagtg aaagttgctg tgattggagc aggtaataga
    11521 ggaacgtatg catatgctcc atatatttat gaaaattctg atgtatgtga aatagttgct
    11581 gtagcagaac ctaaaaaagg aagacgagaa ctatttacac aaaaatataa cctagatagt
    11641 aaaaatgtat ttgaaacttt agaagacttt tttaaacatg acaagatggc agatgcagta
    11701 ataatagcta caaatgatga tagacattat gatgtagcaa aactagctct tgaaaaaggt
    11761 tatcatgtac tattagaaaa gccaatgtct aatagtttag atggtctggt acatatagat
    11821 gacttgtgtg ataagtataa agataagatt tttatgattt gccatgtact gagatattct
    11881 ccattttata ataaattaaa agaaattgta gaaagcaaaa aattaggaga gctagttagt
    11941 atacaatata atgaaaatat aggatattgg cattttgcac atagctttac tagagggaat
    12001 tggagaaata gcaatgaaac cagcccttta attttagcaa agagttgtca tgacatggat
    12061 attttgttat atttagtagg aagtagatgc aaaaaaatat ctgcatttgg aagcttaaag
    12121 catcttaata accaaaatgc tagtggagag atggcacaaa attgtcttca gtgtttagtt
    12181 gaaaaaaaat gtccatattc agcaaagaga atttatttag aaaaagatag aagtataaat
    12241 agagctgttc acataaatcc aactgaagag aatctattaa atatacttaa gacaagtcca
    12301 tatggaagat gtgtttacag atgtgataat aatgttgtag acaatatggt aaatatacta
    12361 cagtttgaaa atggagtgac ggctactttt aatttatgtg catttactaa agaaaatgga
    12421 agaactataa agttaatgtt tagccatggt gaagttggtg gggatttaaa taaaaatgag
    12481 ataagaatta aagaatttgg aaaaaacgaa gaaatagtta tgaatccatc aaatcaacag
    12541 aatatggtag aggaatatga tagaaatctt atagctgaat ttattaaatt agtttcaaat
    12601 aaagaactag aaaagggtag agtagcagca aaagaagcta tacaaagtca tgtaatggca
    12661 tttgcagcag aatattctag ggtcagtgat gaagtagtgt atatagaaga attttttgat
    12721 agtgctaagc aaatgactaa ggagatagaa gaaactatat tttaattaat aaaaatttat
    12781 taaataaaat aaagatagat taaataatag aaatacgaaa atatgtgttc tattaaggaa
    12841 tctatctttt ttattaaggt tttttcttat ccctatcaaa aaaatccata cattctgata
    12901 aaagataaga aagtaaagcg aataagattt ctgtacttaa aggaatcgtt actgaatcca
    12961 taaaggtatc aattatgttc atagctagaa aagttagtat aaaatcaatt ataaattcgc
    13021 aaagtttata tattgagtat ggtaatttgt ttacatatct gaatatatct aaaatagtag
    13081 tgcatagaaa atcaattgga gtggttatac aaaaatatat taaaaagaaa attaatacag
    13141 ctccaagaga tttatattca aatcctaaga atgataagaa gccaattcct ataaccgtga
    13201 aaatacaaaa agttatacca aaaaaaacag caaaaaataa aaatacagaa ataaattcgc
    13261 ctaaaggaat ccttctttta ttacttatct taatcaactc cttaatttat gatatgaaat
    13321 ttatataaat aataacattc ttaaaaattc ataatagtcc tctgtttcaa atagtactgt
    13381 ttgagaatct atttttttca tatttggata aaaagaggcc ttaaaagctt tataatgttc
    13441 tctaaatttt atttctatct taaatttatc tggcaatttt attaagtgcc tagataaatc
    13501 tccagataaa gcttttttga cacctttttc aatttttttt agtgcaaggt ttggatggat
    13561 tgaaattgaa ccatttccaa ttccttttga cacaggaaca gtgactatat ttggatttag
    13621 tttttttgca ctctcacaaa gcatttcatc tccacttaaa aatgctacag gaacattata
    13681 atatgcagca atataagcat ttatagtaaa ttcagatgct atttcatcat ttattttaaa
    13741 atagtcataa tcaatatcca tagtatggga aagtggatta ccattacaag tagctccaga
    13801 gtgatagccg gtgaatatac atgcatcaaa gctttcatca attccagcca tcataactag
    13861 tgggtctttg gtccatcctc taagtatttt gacgttttca ggtaaaatat ttggattaat
    13921 atttctacca ctgtcatgtg catctttaac aagtatttca tgtacaccag attgagtagc
    13981 tccatcacat gctgctgtta tctctttact catttgtgtt ctatagtgtt gcgaaaattc
    14041 acattcaagt tttgtttcat cccaatttac aataccacaa gtaccttcta tatcagcact
    14101 taaatacaat ttcataatat ataccccctt atgtttatta ttgatgataa taacagataa
    14161 cagaaaatac ataatcaata aagggcataa aaatattatg tcttactatt aattattatt
    14221 aaatctgtaa ctgaaaaata actaaattgg tattaaaaaa tataacacta tgtgtcattt
    14281 atttatatag tttttatatc tgatgtttat ataaaattgt aacatataag tggttacatt
    14341 tactagttta acatagttaa aatagttagg tattattttt aagttaaatt gattattata
    14401 aaatcgcaga ataaaagaaa gatttaattt attaaaatgc atgaaatgtt ttttcctcaa
    14461 cataatgcta aattaaactc aaaaagaaat aatagaaaaa tcttatgtag cttagaaaga
    14521 atgtgaatga tttaagggaa aaatagaaat ttattatata atattaggct gtattatata
    14581 atacagccta atattatatg tatatgtgac ataaaatctt ggtaagtgta aataaaataa
    14641 atgtggaaaa caagttaatg aaatcaagaa aaaagtaatt taaaattcaa tttcatcttc
    14701 ccaattaaat tctcctgatt tttctacact cttaggccag tgactacacc atttaggaat
    14761 ggacggattt tcaaccctat caacgcttgg agtatatggc tttaagtcta taacaggact
    14821 attatcatta gcatcaatat atggtatttt tattatacca ttttcatggt cgatattgat
    14881 aatttgtaca gcagtcaatg ctattggatt gggtcttata ggtgaccttg ttgcaaatat
    14941 tcctataacg tctggtccac ttttataagg ttttggggtt tctaaaatac ttcttagttc
    15001 aggattatca aacccatcag cccaccaaaa tacatttaaa tgactgaaat cgtcgagttc
    15061 atttaatgca ggtacatact ttttgtctaa tttaataaag acctcttcgc cttgaataca
    15121 tattttacct ataggattta ttaatatttc tttcataata ttacctccaa ttatttaatt
    15181 tatcatttgt aattttaggg tataacatga catcatgtga gggtcaatat aaaataataa
    15241 ataaaaatat taatagttag aatgataata cattttttac atggagcatg acaccagtaa
    15301 taaggcaatc ttcgtcaata tcaaaagtag aggtatgcaa aggggaagta atgttttttt
    15361 cacgatttgc acaaccaagg tggtaaaaag ctccttcaca gtgtcttaga aaatacgaaa
    15421 agtcttcagc cccaagagtg ggcgagtctt taaggataat attttcttct ccaaacaatt
    15481 ctacagcatt ttgtttgacg taatctacta gatggtttga attgataaga gctggataac
    15541 cttcctcaat ttcaacagaa atacttcctc caaatgtttt acaagtaaaa tcgcaaattt
    15601 ctctaattcg tttttttgaa aactctctag tttcaggagt caaggttctc aaagtacccc
    15661 ttataaccac tttatcacaa actatatttt ccttgattcc accttcaatc tttcctagag
    15721 aaataaccac agaatcagtt ggatttgtgt ttcgactaac gattgtctgt agagctgtaa
    15781 taatatgagc agatataaca atagcatcaa taccttgatg tggataagct ccatgacatt
    15841 ttgaaccatg tacagttaat tgaagtgtat cagtggaagc atttagagtg ttgtatttta
    15901 attcaattag atttttgttg atattggaat ttacatgaag tccaaatata tattttacat
    15961 ttgggttttc aagacatcca tcttctatca taagttgagc cccaccaata gtttcctcgg
    16021 caggttgaaa aaagaatttt acatttacat ttaagtaatc ctttaattta aatagtatat
    16081 tacaagtacc cagtaaaata gcagtgtgtg catcatgacc acatgcatgc attttgccaa
    16141 tattaataga tttataagaa taattaagtt cttctgttat aggtaaggca tctatatcag
    16201 ctcttatagc aactgtttca aagtttggac tcacgttaat gtatgcagtt atccctgtat
    16261 gattcttata ttcaatataa tttataccta tttcatttaa gtatttaata atttttttct
    16321 tagtttgaaa ttcttctaat ccaagttctg gagtcttatg taaatctctt cttatattta
    16381 taacccaatc atttatctca tgacaaagag tttttatatt aagcataaaa cacatcctaa
    16441 ctatattaaa aatttatttt tcttcattaa ttatattatt ttatgtaaat cattattatg
    16501 tatcaagtac aaataaaata tcttataatt atattagaga tatttagtat ggagaagaat
    16561 atatcatttt ataagaagag gtgtttaaat gttaggaata attgcgccat ctgggccatt
    16621 aagaaataca agtttggagg aaataaagtt taatttagaa agttatggtt atgaagttaa
    16681 gttttctgaa agttgtagtt taaattataa aggttattta gctggtaatg atgatattag
    16741 agcaagggat atagaagata tgtttttaga taaagatgta gatataatta tgtgcttaag
    16801 aggaggttat ggaacaacaa gaatattgga taaaataaat tatgatataa taaagcaaaa
    16861 tccaaaacca tttataggtt tttctgatat aacaggttta aatttagctt tctataaaaa
    16921 ttgtggtctt ttaccatatc atggaataat ggcagcaaat gtaggtaaat gggataaatt
    16981 tacctataaa tcattggtaa atgctttaga atttaaagat gaattatatt tagaaaatcc
    17041 aaaagaagaa aagatttata ctgtatgtga aggtaaagca gagggaataa taatgggagg
    17101 aaatttatcc ttaataattg ccacaatggg aacaaaatat gaaatagatg caaaagataa
    17161 aattttgttt attgaggaaa ttggagaacc tcaatataaa ctggatagaa tgttaacaca
    17221 actatattct tcaggaaaac ttgaggaatg taatggtata atttttggag attttaagga
    17281 ttgtattgaa gaaaatgatt tgatggaatt actaatagaa tttgcaaata aggtcaataa
    17341 accttctata tataatcttc agtcaggaca ttgtatccca atgataacta ttcctttggg
    17401 cagaatgtgc gaattagatg ctacagaaaa aatcgttaaa ttaaaaaaat agtgtgtata
    17461 taaatataat agaaatatgg tataatctta tacaagtaaa aatttagcta ggaggatgaa
    17521 aaatgtctga tgatagaata atagacttta acgaactaaa gaacaaagtt aaagattctg
    17581 atgtagataa atttgagcaa tacatatata acttatattt ttctgttatg gatggaaaga
    17641 tgtctatggc tgagttttca agaaaaatat tcgattatat gagagataac aacatatctc
    17701 aagaaaaatt tatgaaaata caaaagcaat ttatggagag atatggtatg gacactgaag
    17761 aagtagaaaa acaacttaga aactttggaa tcgacccttc tacggcaggt tttatgagta
    17821 ataatacttc cagcaaagta tctacagaag atttagagtc atttaagaag agtgcaggat
    17881 tttatgagaa atatggagaa aaaatccaac caaagagttg tataacaaca tttataaaaa
    17941 atgacttaaa tgatattaat gtaataatcg accaagaaaa gataatgctt tgtagtgata
    18001 gaaaaataaa tcttatggat tcagaactaa atgagttttt attagaatat aagaatatgt
    18061 ttaataaaaa aataaaggta gtaatgtgcg aaactacgaa taagtatgat tattaaaaaa
    18121 tgaatttaaa tactgaagta gtatcaatta attgtactac ttaaaaataa aaatcgcaat
    18181 ttattattcc ttataaattt gtataaggaa tatctaaact gcgatttttt atattgtgaa
    18241 gattagtaat aatagatagt gttttaaatt gtaatcttct cactaaaact gtgtttttct
    18301 attagaaatt ttattaaaaa taaatatctg ataattgtat tatatgggat gtaaagtatt
    18361 agaagcttaa cgaatctctt ttaatacatt aactccattt tgaatcatac tgttgaaaaa
    18421 tactaagttt aaaatctcag cagggtgaac gttaggaata tcataagtat ctactaaatt
    18481 cataggtaca actatatttt tttcaatatt atgttgatta aaatatgatt ttagagttat
    18541 tgcaaattga tatatacata tatctgtgca atcgccaact ataattatat tatctatatt
    18601 atcaaaatcc aaattttcta atgcaaaaaa accattagta gaatttttag gaagaatttg
    18661 taggttttct atcgacttaa gttcatcaac caattcactt tctacatcat tttccaaaca
    18721 gtgaacagga taactcaaaa gttctattga tttaggagta tgagaatcag taaatgctat
    18781 tactctattt aatttgtttg atattttttt tgtaaacatt tcaattggct ttattaaaga
    18841 ctctactcta ggagagtata atgcaccttg tcttgcaaag ccattattaa catcaattat
    18901 aaataaagca gttttactca aatcataatt ttctattttt tctattggta gattatctaa
    18961 attactcttt aaagcttcaa gttcatttaa taagttatcc atagttatac ctccaagata
    19021 aatttaataa aattgtacat attaattata taaaagtcaa ggagtacaaa ctattgtata
    19081 attagctgta ttgttttgag tgtaaaagat ttgaagcaat atctggatag ttagtaaaaa
    19141 taccatctac attgagtttt agacagtgta gtatatcatc ctcttcattt acagtatata
    19201 cattgacacc aagtagattt ttgtgacaaa gttcaattag agattcattt actaaaacta
    19261 gaggtggatg aagataacta ttacccagaa atttagcaaa attgactaca ttgtttatag
    19321 gtaaataaca caataatcct gtttttacac tagggtctaa tttatgaaag ttttttaagc
    19381 tattgctgtt gaaactagat ataagaactc tatttttcat attatacttt aaaataagct
    19441 ctaaaacgcc tttttctata ttgggatagt ttattttgtc agtttttagt tcgatattta
    19501 aagtaaggtc tgtaggtgag attagaatta atacttcttc taaagttgga attttacatt
    19561 ctttattgct ttgatatcct tcaaaagatg agtttagagt ttttaatttt cttaaagtaa
    19621 agtcttttac aaaaccatgt ccattaaaag ttctatcaac cttttcatca tgaataacta
    19681 caatttttcc atccttagtt ctgtggacat caagttctat tccatatata tccatatcca
    19741 aacatttttt aaaagctaga atagtatttt ctggatactt tccactaaac cccctatgag
    19801 cataaatatt catttatatg gttccctcct ataagtataa cttatcttat aaaaatttaa
    19861 ttaatataat atatcatatt ccaaaaggta ttgttatata ttagttaaat aactatttgt
    19921 aatttatata ttatataaat gtagaaaaat aaaatcatat atgtgttata atatatttta
    19981 aacattgtaa atattacgaa tttagtagtc ataataagtg taattttatg caagctcatg
    20041 tttttagagc ttgcatatgt attattaata tatttatata ttgtgaaaat aaaattccaa
    20101 aagaattttt tctatagact tagtagtaag ggggaatttt gatatgtatg aagttaattt
    20161 agaacgagct atgaattatt ggatggattt gttagatgat tatacgaata tagcaaaaat
    20221 tccaaatgat tttttttata aagattgttc tgatgaagta gtagatgagg tttttaagat
    20281 gaatattgag aaacatgaaa ggttaattca atattctaaa gtgaacaata ttagcataga
    20341 ctctatcata gaaactatat atggccttat acttcaaaag tatatatatg aagatgatgt
    20401 agtttttggt aaaacaatga atataattcc aataagagta agagttagac aaagtgaaaa
    20461 atttatagat gttgtaagga agctaaaaag gcagtggagc aggtctttag agtatggata
    20521 ctatttgtta tcagatatag agaataaaaa tcatctaaat actaagctta taaacacaat
    20581 atttatagct aaaagttttg atttatcata tgattatgac atagaaaaaa aaggatatga
    20641 tttatctgta atagtatata ctaagagggc acttaaaata aaaataaagt ataaaccatc
    20701 tatatataaa agagaaacta ttagaagaat tttaaatcaa tttgattata taatagagca
    20761 aataatagaa gataatgata ttttaataga agatttagaa tttgtatgta aggaagaaga
    20821 aaaagtatta ttggaagact ttaataataa tcaatatgaa gtaccctatg ataaaagtta
    20881 tgttgattta tttagggagc aagttaaggt aacacctagt aatatagctg ctagggatga
    20941 aaatagaaag ttaacatata aagaattgga tgaagtaact gataaacttg caggttattt
    21001 aaatcatata ggagtggaaa gtgaagacat agtagctgta atgttaccaa gagatattaa
    21061 cataattatt acagcaatag ggattatgaa atcaggggga gcattttttc caatagacac
    21121 aagtaatcca gaagaaagat tgaattacct tcttgaagat agtaatgcga aagtagtgat
    21181 aactacagat gaactcaaaa gtaaggttgt aaatgagaat accatagtac tcgatataaa
    21241 tgatgaagaa atgtttaagt ttggatatga attaactgaa aaaataacat catcaaattg
    21301 tgcatatacg atatctactt cagggtcaac tggtaggcca aagaccatag ctatagaaca
    21361 taagagttta gtaaatatgt gttattatag cgtaaaatct ataagcgcta cagaaaatga
    21421 tatatgtggt atatacttaa gtttcagttt tgatgctgta atgaaacaac tattccctta
    21481 tttgcttgta ggtgcatcaa tagatattat gcctgaagaa gctaaatttg atgaatatac
    21541 agtaaatgaa tactgtgagg aaaatgacat aactatacta gcattgccaa cagcatttgc
    21601 aaggttattt atacaaaatt gcaataataa ttcacttaga gttgtacaga ctggaggaga
    21661 aagattaaaa ggatatagaa agcgtaatta tgaactatat aatgagtatg gaccaaccga
    21721 atttactgtt gtatcgactt cttttcatgt ggacagagaa tatggaaaaa taccaatagg
    21781 aaaacctata tttaatactt atgcatatgt attagataaa aagaacaaac tatgtccaat
    21841 tggagcacca ggagagctat gtctatcagg cattcaaata tcaagaggat atttaaataa
    21901 aaaagggcta acagagcaag tatttgtaga gaatccttac aaaacttgtg aacataataa
    21961 gttaatgtat agaactggtg atttagtaag atggttagat gatggaaatt tagattatat
    22021 tggaaggatg gacaatcaag taaaaattga tgagtttagg atagagctat atgagattga
    22081 aaatataata aataatatta cagaaattaa aagtgttgtt tgtatatcga gaacaaatga
    22141 tgatggagat atttatatat gtgcatatta tgtaattgat gaggaagata gtggtaagat
    22201 aaatgaaaga actattagag aatatttaaa tgagcatcta cctccatata tgattccaac
    22261 aataataatg aggatagata agataccagt tacaccaatc ggaaaagtaa ataaaagagc
    22321 attaccagaa taattttttt attatgatag gtgtcataaa gtgatttcag acatttcttt
    22381 atgacactta tttttataaa ataatcaatt tgcataaaaa caagaatttt ctgtatatat
    22441 ttactatggt gaaaatttat ttataagaa
//
LOCUS       DAYAPA010000062        22132 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.062, whole genome shotgun sequence.
ACCESSION   DAYAPA010000062 DAYAPA010000000
VERSION     DAYAPA010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22132
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.062"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            444..1514
                     /locus_tag="ACGI59_002150"
     CDS             444..1514
                     /locus_tag="ACGI59_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9739936.1"
                     /translation="MADIRLENVTKSYNKKNTVIENLNLTIEDGSFTVLVGPSGCGKS
                     TTLRMIAGLEDITSGRLKIEENDVTKTDASKRDIAMVFQNYALYPHMTVRENIEFGLK
                     NKKVEKEKREKLITEVIDVVGLREYLNVKPGNLSGGQRQRVALARAMVKNPKVFLMDE
                     PLSNLDAKLRNQMRTELIQLHQKLKSTFVYVTHDQVEAMSMADKIVIMNKGKIMQIGS
                     PKKIYQNPKNLFVAQFIGSPCMNILDIKNDIKFGFRPEKATLESINIDGKNDDVFSLF
                     GKVVTKEVLGNETIYCLSVLNREIRVKTENDIFDIGRTLRISVLEKDLYYFDKNGERI
                     EDVKVCKSMYSKIKGDIYEKAI"
     gene            1498..2367
                     /locus_tag="ACGI59_002151"
     CDS             1498..2367
                     /locus_tag="ACGI59_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFG9739937.1"
                     /translation="MKKQFNKAYLYITPAIIGTLLFIVYPIIKTISLSFKSGSLLSST
                     FENVGLNNYTELLKNPEFIQTITNTAIYTFFMVTISISLAIVLAVWLNKNSIIHNLTQ
                     SIIFTPHVISLVSVAVLWLWIMEPQYGFLNWILSTLHLPTSKWLSSESSALMSLILIG
                     IWKTLGYNILIVLSGLKSIPNYIYEASKLDNANKITNFFKVTLPLLSPTIFFLMITTT
                     TASFQVFDEISIMTQGGPVGSTNMLSYYIYESGFIYYDIGQASAASMFLLAIVMIATY
                     ANFRLLSKKVHYQ"
     gene            2411..3271
                     /locus_tag="ACGI59_002152"
     CDS             2411..3271
                     /locus_tag="ACGI59_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFG9739938.1"
                     /translation="MDIIQPIKKDKVDIKKIASKSIEILILLLLSAIFIFPFIWMLST
                     SFKNPQEMMQLPPTIIPNDIVLENYSTAWHSGPFFRYLLNSVFVTGSVLIVQFLVMVP
                     AAYAFSKIKFKGEKMLFGLLLVGLMIPPQVTFLPVYMMMSKFGFINTYVPLIIPFMTS
                     SFGIFLLRQNFMQISDEIIEAAKLDNASDLKIMFRIMVPMAKPAVITFILFNFIYHWN
                     NYFWPLVMTNTDAIRTLPIGVALLKSSEGITAWNVIMAGNIILILPIILVYIFANKKV
                     KEAFMYSGIK"
     gene            3302..4606
                     /locus_tag="ACGI59_002153"
     CDS             3302..4606
                     /locus_tag="ACGI59_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFG9739939.1"
                     /translation="MKLKRKLATGLAITLLLCSLVGCSNSNDANASKGEKTDSNITKI
                     DFWAPLGGTNGETAQKMVDQFNSEHKDVQVNMLKQKDYYENATKLQAALTSKDQPDVT
                     LLEITQTGTFASAGALVDMSKYFDKTYQERFFSGLLTNSYYEDKFVGMPFNRSTPILY
                     INKDMATKAGLDPSGPKSWEELKDYASKMTNKSEDTYGFETPIDIWFYEAMVMQSGGE
                     ITNGKKVAFNNEAGQAPVKFWQDMMKAGIMKMPPGEDYNAWDVAKQDFVNGKVGMIFT
                     STADLAGLMQQTEGKFEISTAFLPKNQKYATPTGGANLVMLEGGTDKEKEASAEFIEW
                     MTQTDKIVQFSSSTGYLPTTEDATNSEKLQTLYKEKPQYKVATDQLQYAVALPMLNGY
                     KEATDKLMDEIKKGLTDLNANPKDVVSKGTSAMQAVIDKTNK"
     gene            4669..5931
                     /locus_tag="ACGI59_002154"
     CDS             4669..5931
                     /locus_tag="ACGI59_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HFG9739940.1"
                     /translation="MTKVIVLSVDSLFEKDLEFVKNLPNFKSILENCSIVKNINCVYP
                     TLTYPCHTSMVTGVYPKKHGIYHNEKFDPNKENKDWYWYSKDIKAKTIIDVAKENNLT
                     TSSVLWPVMGANPNIDYNIAEIWAPSREDDPRETFERSSSKVIMDGIYNRHCHYIDWK
                     LEPNMDLFGVNCSVDIIKEYKPDLMLIHLATLDHTRHNYGLFNDEVDKALKMNDKWLG
                     DIIQATKDAGTYKDTNFIILGDHGHLRVDKVINPNVLLKSNGFIKVENGEVKDFDAYV
                     NSSGISAQIIVNNLDRLTELRELLEEFKEELFIENIFTKEEASNLGLEGDFEMVIEGL
                     EGISFGNDFEGSIIKDSDIKDYKFAVATHGHLPTKGNRPPFIAFGPNIKGGVVIEEGD
                     LRAHAATILKMFNLKLDGVEKEAFDFIM"
     gene            6118..8088
                     /gene="uvrB"
                     /locus_tag="ACGI59_002155"
     CDS             6118..8088
                     /gene="uvrB"
                     /locus_tag="ACGI59_002155"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFG9739941.1"
                     /translation="MDFKIKSDFKPTGDQPEAIKSIVDSINRNEKFSTLLGVTGSGKT
                     FTMANIIQQVKKPTLIMAHNKTLAAQLYSEFKEFFPDNAVEYFVSYYDYYQPEAYVAH
                     SDTYIEKDASINDEIDKLRHSATASILERRDTIIISSVSCIYGLGDPKDYKELMLSIR
                     PGMQRDRDDVIKRLIEIQYERNDINFTRGTFRVRGDILEIFPASNDEKAIRIEFFGDE
                     VDRITEIDYVTGKIVGTRNHVVIFPASHYVTTPERIEKAIVEIENELQEQIKFFKEND
                     RLLEAQRIEQRTKYDIEMLKEIGFCQGIENYSRHITGRSEGERPYTLMDFFPDDYLII
                     VDEAHVTIPQVRGMYAGDRSRKTSLIENGFRLPSALDNRPLNFQEFEGNINQMLFVSA
                     TPGPYEIQHSETIAEQIIRPTGLLDPIVEVRPINNQIDDLVGEITKTIEKNERVLITT
                     LTKKMSEDLTNYLKEIGIKVKYLHSDIVTLERTEIIRDLRLGKFDVLVGINLLREGLD
                     IPEVSLIAILDADKEGFLRSETALIQTIGRAARNENGRVIMYADRITDSMQNAIDETK
                     RRRDIQNLYNEEHNIIPKTIQKNIRDSIEATKVAEEEVVYGISDTDDKDEIRANIDKL
                     KSEMMEAAQNLQFERAAELRDKVKQLEEKLEK"
     gene            8107..10932
                     /gene="uvrA"
                     /locus_tag="ACGI59_002156"
     CDS             8107..10932
                     /gene="uvrA"
                     /locus_tag="ACGI59_002156"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFG9739942.1"
                     /translation="MEDKIIIRGAKEHNLKNIDLELPRDKFIVFTGLSGSGKSSLAFD
                     TIYAEGQRRYVESLSAYARQFLGQMEKPNVEYIEGLSPAISIDQKTTSKNPRSTVGTV
                     TEIYDYLRLLFARVGDVYCPTCGEKISQMTIQEIVDKMLDFPDRTKLQILSPIVRGQK
                     GTHKKVFDNIKKEGFVRVRVNGENYEVSDDIILNKNQKHNIEVVVDRIVIKDGIESRL
                     ADSIETAVKLSDGLVIAQIIDGEEILFSTKFACPEHGIGIEELSPRMFSFNAPFGACD
                     VCNGLGESKEVDPELVVPNKDLSIKQGAVAAWGSTGVNDDTYYSKMIKSLAEHFNVSL
                     TTPFKDLPDDFVEELLYGKDNIIVEFTYESKFGGTRNYKSYFEGVIVNLERRYRETNS
                     DYMRDKIEEYMAERPCPKCKGMRLKKEVLSVLVDGKNIMEVTNLSVNELIDFMENINL
                     TEKQRFIAHEIIKEIKGRAMFLRDVGLDYLNLSRKAGTLSGGEAQRIRLATQIGSALV
                     GVLYVLDEPSIGLHQRDNDRLITTLRHLTDLGNTLIVVEHDEDTMREADYVIDIGPGA
                     GIHGGEIVAQGTLDEIIENENSITGQYLSGKKQILLPETTREGNGSFIEIVKASENNL
                     KNIDVKFPLGKFTCITGVSGSGKSTLINDILYKGVASKVNRLKQRAGKHKEIKGIESI
                     DKVINIDQSPIGRTPRSNPATYTGVFDFIRDIFASTNEAKARGYKKGRFSFNVKGGRC
                     EACKGDGIIKIEMHFLPDVYVPCEICKGERYNRETLQVKYKDKTISDILDMNVEEAVE
                     FFENIPNIKRKLETLMDVGLSYIKLGQPSTQLSGGEAQRIKLAAELSKRPTGKTLYIL
                     DEPTTGLHMADVDKLIQVLQRLADTGNTIIVIEHNLDVIKTCDYVIDLGPEGGDKGGT
                     IVATGTPKEVSKVDGSYTGQFLKKYFS"
     gene            11191..13008
                     /gene="uvrC"
                     /locus_tag="ACGI59_002157"
     CDS             11191..13008
                     /gene="uvrC"
                     /locus_tag="ACGI59_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFG9739943.1"
                     /translation="MFDIQEHLKKLPSEPGVYLMKDKYDHIIYVGKAISLKNRVRQYF
                     QSSKNHTSKVKSMVKNIYKFEYIITDSELEALILECNLIKRYRPKYNVVLRDDKTYPY
                     IKVTTNEDYPRILKVRRVLKDKAKYFGPYTNITAVNDTLELISSTYPIRSCKIDIDKA
                     IKNKTRPCLNLHINKCLGPCTGNVSKEEYGKMIEEIIMCLSGKEEKLMELLKEKMNES
                     SMNFRFEEAAVYRDKIKSLEEMIQKQKIDATVSDLNQDVVAMARAHNEACVQVFFIRN
                     GKIVGREHFILEGVMDSPRASILSSFVKQFYNEQEYIPKELIIEDEIEDSSILEEWLS
                     SKKGQKVTIRVPQKGEKKSLVEMVRKNAVEYLEKFSDMNKRKYEKSIGALEELKQILN
                     LEKLPIRIEAYDISNIQGVDSIGSMVVYTNAKKDKKEYRRYKIKTVIGPNDYDSMAEI
                     VDRRLKHGNLPDLILLDGGKGQVSAVKKVLELNDVDIPLWGMYKDDKHRTKGLICKEK
                     EIELDKTTNLYRFIASIQEEVHNYAITYHRSLRNKALTKSILDDIQGIGEKRKKSLLN
                     HFKDVDAIKKATMEELLEVDGMNKSIADNVYNFFRKEEN"
     gene            13008..13937
                     /gene="hprK"
                     /locus_tag="ACGI59_002158"
     CDS             13008..13937
                     /gene="hprK"
                     /locus_tag="ACGI59_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HFG9739944.1"
                     /translation="METSNKVSIREIIKELKLKVVYMPDDVDYYVYSQDINRPGLQFA
                     GYFDYFAYERIQIVGKTEYNFFGLMDSHIREEVLDRFFSYEIPALIISRDLEIKPDVI
                     EKAKKYKRILLSTNRNTTRLINRLSNYLDNKLAPHTTIHGVLVDIYGIGVLIKGESSI
                     GKSETALELIQRGNRLVADDAVEIRKLDESMLVGQSPELIRHFLEIRGIGIIDVRSLY
                     GVGAIKNSKMIDLVVHLEAWDETKYYDRLGLDKEYEEILGVNVEKLVVPVKPGRNTAM
                     ILEVAAMNFRQRGMGYDAAQEFTKKLSKLIDTK"
     gene            14149..15945
                     /locus_tag="ACGI59_002159"
     CDS             14149..15945
                     /locus_tag="ACGI59_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate oxidoreductase"
                     /protein_id="HFG9739945.1"
                     /translation="MGIEYFSKLLESSTEKELLLSKKSNNISTARLISFLIVIAGLAI
                     GFYNKNIVGIFVGVLSIIIFIALLVIHSKVKEEETYFKSKSEVLNKYIKRFGDGWKEF
                     KIDGKEYLKDENSQAKDLDLFGRASLYQYICVANTSYGKNFLAKYLWNENPDENIILE
                     RQRAIKELLSKDDFSIHIQTLSNIIGKEQKNNSDSSIESFIEYGENKNVYIPKWMHIF
                     TWGLPTATILSFIFCMLGFLPVLPVFLLFIIQLGFSGFGYPRLMQTLAPLFSFSRSIQ
                     VYEKMFEVLEKETFESAYLKELQDKLSKGSGVSRGVKQLNSIGNAVNLRYNQILYIIA
                     CGVLMWNYHCAEALERWKGIYGNQIRDWFESIGEFEALISLTVVSHVKENTCFPVIKY
                     EDTPRLKVEEVYHPLIAEKSVIANSIQLNSQTCIITGSNMSGKTTFLRSIGVNLVLAY
                     AGAPVCAKSFDATCMAIFTSMRIQDDVSQGISTFYAEILRIKSMIQYSLKELPMLVLV
                     DEIFKGTNSADRIIGASEAVKKLSKPWVISMVTTHDFELCDLSGSGDVEVVNYHFSEY
                     YVDDKIHFDYKIKDGRCKTTNAKQLMRMAGIL"
     gene            complement(16085..17866)
                     /locus_tag="ACGI59_002160"
     CDS             complement(16085..17866)
                     /locus_tag="ACGI59_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HFG9739946.1"
                     /translation="MSLVNLTINGKHVSAPSGTSILDAAKLINIKIHNLCHLHMNEID
                     KLDTCASCRVCMVETERGLVPACGTVIKEGMKVQTNSAKALNARRTIVELLLSDHPQD
                     CFVCEKNGDCELQTIAADLGVRKIRYQGSKSFAGKDTSTKSLVKDHSKCILCRRCETV
                     CNDIQTVGALSGVNRGFNTLVSTFFNSDMVETECTFCGQCISVCPTGALTEVDNVPKL
                     WDVLNKKEKTIVVQVAPAVRVAIGEEFGLEPGSISTGKMVAALKALGFEHVFDTNFGA
                     DFTIMEEATEFIERIQKGENLPILTSCCPAWVNFLEHNYPDKLNLASSCKSPQGMFGS
                     IAKNYYAPKILGINPEELYVVSVMPCVAKKYEASREELSESGILDVDLSITTRELAKM
                     IKEAAIDLPNLQDQDFDNPLGKSTGAASIFGASGGVLEAALRTSYEKITNKTLDNVNF
                     TNVRGLKGIREASIDVDGTTVNVCIVNTLKNARKIMDKVRSGECKYHIIEVMACPGGC
                     VGGAGQPYHHGNTEIVDRRANALYEIDRNKAIRKSHENPDLQAIYKDFFGEPNSDVAH
                     KYLHTHYFDKSCVYGECPQECACEEAK"
     gene            complement(17889..19775)
                     /locus_tag="ACGI59_002161"
     CDS             complement(17889..19775)
                     /locus_tag="ACGI59_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HFG9739947.1"
                     /translation="MRKVNSFDELKVLADELKPNLSLRKNYSKENVLRRELLVCCDTG
                     CTSSNSLEIVSELENEIKKSGIQDKVSVRLTGCFGFCAQGPIVKVYPDNVFYVKVEPS
                     DAEKIVQSHLIRNTVVEELLYEEESLSKKVEEQEEMSFYKKQLRIALKNCGLIDPDSI
                     EEYIANDGYLALGKCLTSLTPQEVIAEVKTSGLRGRGGAGFPTGSKWEAASKNPAGPT
                     DKKFVVCNADEGDPGAFMDRSVLEGDPHSVLEAMAICGYAIGSDTGYIYIRAEYPKSI
                     ERLKVAIAQAEKYGLLGENILGTGFNFKLELKYGAGAFVCGEGTALMHSIEGRRGEPR
                     MKTYSSSKSGLWKSPTCLNNVETFANIPAIILKGGDWFANLGTEDSSGTKVFALGGKV
                     ENVGLVEVPMGTTLRDIVFEIGGGIPEGKKFKAVQTGGPSGGCIPTEHLDTPIDFGSL
                     SSIGSMMGSGGMLVLDESDCMVDIAKFFLEFTVEESCGKCTPCRIGTTRLLEILTKIT
                     EGNGTLQDLDDLENLAETIQTASLCGLGKAAPNPVLSTLQYFKDEYIAHVVDKKCPAG
                     KCQNLLSYFITDDCKGCTKCSRVCPAGCITGSVKEQHTIDTSKCLKCGACIDNCTFNA
                     IIKK"
     gene            complement(19792..20289)
                     /locus_tag="ACGI59_002162"
     CDS             complement(19792..20289)
                     /locus_tag="ACGI59_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HFG9739948.1"
                     /translation="MCDFISHNKQLFDELDIFIDSLATKEGALIQVLHEAQGIFGYLP
                     KEVQLHVARKLGVAPAKVYGVVTFYSYFTTEPVGKYKISVCLGTVCFVKGADKILSAF
                     EKQLGIKVGETTSDFKFSLEGLRCLGACGLAPVVTVNGKVYGKVKPDQVSEILDTYRE
                     LELNC"
     gene            complement(20607..22088)
                     /gene="cls"
                     /locus_tag="ACGI59_002163"
     CDS             complement(20607..22088)
                     /gene="cls"
                     /locus_tag="ACGI59_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFG9739949.1"
                     /translation="MFDISFFEMSIYEIAVTTIYIINFMVILNLIFREKRNINTTLTW
                     LLILVLIPALGFILYIAFGRNISKNNMFRLKEKDDKIIKSNILDTQVKLQSTSEIDSD
                     IHQHKDMIYALANSNNAHYTNNNDVWIYAESSQFFNSLLEELKKAKKYINIQFYIFKD
                     DKIGTEIIDILIDKAKEGVEVRLLFDAVGGRTLKNSTLSRLKESGVKVGSFFPSFLKI
                     VNFNLNYRNHRKIVVIDGKVGFVGGYNVGDEYLGRNPKFGLWRDTHTKLTGDCVIDLN
                     MRFILDWRYTTKEDLDLEKYFIEEDTNSNHPSENIGIQIVSSGPDISELDEIKYGYLK
                     MIQKARKYIYIQSPYLILDSTFIDTLKIACLSGVDVRVMIPSKPDHPFVYWASYSYAG
                     ELLKFGAKIYTYGQNAFLHAKTIVIDDSICSIGTANMDIRSFELNFEVNAFMYSSKKA
                     LEQRIIFENDILNSKEITLDIYNSRSTYIKIKESISRLLSAVL"
BASE COUNT         8511 a         2967 c         3695 g         6959 t
ORIGIN      
        1 ggtaaattta taaaaattaa aaaattttta ttttttatgt ttaaaccttg taaatttggg
       61 acataatata attgtaaaac ttaatatagc aaaaataaat tactcaatct cccccactta
      121 aagacctcta tcccccatag aggtcttttt tactttcaaa ccaaattata taaaaaataa
      181 atagttttat atgactcttc ataaattaaa attacattgt gatagcaaac catgaatatg
      241 aaaaatatag aaaattatag ctaacataag ctgtatatca tcttaacttc agtatattta
      301 acaaaaaatg tttataattt aacctttatt ttaataagtt taccatatat tcactttgtc
      361 ttaatatatc ctttacaaat taagagtatt attaatctat aagtaaatcg tagaattaaa
      421 ttaaatattt tgggaggata aaaatggcag atataagact agaaaatgta acaaaatctt
      481 ataataaaaa aaatacagtt atagaaaatt taaatcttac tatagaagat ggttctttta
      541 cagtattggt aggcccaagt ggatgtggta aatctactac gttaagaatg attgcaggtc
      601 ttgaagatat aacttcaggt aggttgaaga ttgaagaaaa tgatgttaca aaaacggatg
      661 ctagtaaaag ggatatagct atggtatttc aaaattatgc attatatcca catatgactg
      721 ttagagaaaa catagaattt ggtcttaaaa acaaaaaagt agaaaaagaa aaaagagaaa
      781 aacttataac agaagttatt gatgtagtag gattgagaga atatttaaat gtgaaaccag
      841 gcaacttatc tggaggtcaa agacaaagag tggcacttgc tagagctatg gttaaaaatc
      901 caaaggtatt tttaatggat gaaccattgt caaatttaga tgctaaatta agaaatcaaa
      961 tgagaacaga attaatacaa ttacatcaaa aactaaagtc tacatttgtt tatgtaactc
     1021 atgaccaggt tgaggcaatg tcaatggcag ataagatagt tataatgaac aaaggaaaga
     1081 taatgcaaat aggttctcca aagaagatat atcaaaatcc taaaaattta tttgtagctc
     1141 aatttatagg gagtccttgt atgaacatac tagatataaa aaatgatatt aaatttggat
     1201 tcagaccaga aaaagcaaca ctagagagta taaacataga tgggaaaaat gatgatgtat
     1261 ttagtctatt tggaaaagtt gtaactaaag aagtgttggg aaatgaaact atatattgtt
     1321 tatctgttct taatagagag ataagagtca aaacggaaaa tgatatattt gatattggaa
     1381 gaactttaag aataagtgta ttagaaaaag atttatatta ttttgataaa aatggagaaa
     1441 gaatagaaga tgtaaaagta tgtaaaagta tgtattctaa aatcaaagga gatatttatg
     1501 aaaaagcaat ttaataaagc atatttatat ataacacctg ctattatagg aactttattg
     1561 tttatagtct atccaataat taagacgata tctctaagct tcaaatctgg tagtcttctt
     1621 agttcaacct ttgaaaatgt aggcttaaac aattatacag aacttttaaa gaatccagag
     1681 tttatacaaa ctataacaaa cactgcaatt tatacttttt ttatggtgac tatatctata
     1741 tcactagcca tagtacttgc tgtttggcta aataaaaatt ctataataca caatttaacg
     1801 caaagtataa tctttacacc acatgtaatt tcactagtat ctgtagctgt attgtggctt
     1861 tggattatgg agccacaata tggcttttta aactggatac tatcgacatt acatttacca
     1921 acatcaaagt ggctttcaag tgaaagttca gctttaatgt cactaattct aataggaata
     1981 tggaagactc ttggatataa tatcttaata gtgttatcag gtcttaaatc cataccaaac
     2041 tatatatatg aagcatctaa attagataat gcaaacaaaa taacgaattt ctttaaagtg
     2101 acattgccac tattatcacc aacaatattt tttcttatga taacaacaac aactgcatct
     2161 tttcaagtat ttgatgagat aagtataatg acacaaggtg ggccagtggg ttcaactaac
     2221 atgttatctt attatatata tgagtctgga tttatatact atgacatagg gcaagcgagt
     2281 gcagcgagta tgtttttgtt agctatagtt atgattgcta catatgctaa ctttagactc
     2341 ttatcaaaga aagttcatta tcaataaaga aaaagtagat attataaggt ttatccaaaa
     2401 ggaggagaca atggatatta tacaacctat caaaaaagac aaagttgata taaaaaaaat
     2461 agcatcaaaa tcaatagaaa tattaattct attactttta tctgcaatat ttatattccc
     2521 ttttatatgg atgctatcta catcctttaa aaatccacaa gaaatgatgc aattaccgcc
     2581 cactattata ccaaatgata tagtgttaga aaattatagt acagcatggc attcaggtcc
     2641 attttttaga tatttgctaa acagtgtatt tgtaacagga agtgttttaa ttgtacaatt
     2701 tttagttatg gttccagcag catatgcatt ttctaagatt aaatttaaag gtgaaaaaat
     2761 gctatttggg cttttgcttg taggacttat gattccacca caagtaactt ttctaccagt
     2821 ctatatgatg atgagtaagt ttggatttat aaatacttat gttccactta taattccatt
     2881 tatgacatct tcatttggga tatttctgct tagacagaat tttatgcaga tttcagatga
     2941 aattattgaa gcagctaaac tagataatgc ttctgactta aaaataatgt ttagaataat
     3001 ggttcctatg gcaaaaccag cagtaataac atttatatta tttaatttta tataccattg
     3061 gaataattat ttctggccct tagtaatgac aaatacagat gcaataagaa ctttaccaat
     3121 aggagttgcg ctccttaaat cttcagaagg aattactgca tggaatgtaa taatggcagg
     3181 aaatataatt ctaattttac caataattct tgtttatata tttgcgaata aaaaagtaaa
     3241 agaagctttt atgtattcag gaataaaata aaaaattaaa gactaaattt ggaggatgaa
     3301 aatgaagctt aaaagaaaat tagcaacagg attagcaata acattattat tatgttcttt
     3361 agttgggtgt tcaaattcta atgatgcaaa tgcatctaaa ggagaaaaga ctgatagcaa
     3421 tattacgaag atagatttct gggctccttt aggtggaact aatggagaaa ctgctcaaaa
     3481 aatggttgac caatttaata gtgaacataa ggatgtacaa gtaaatatgt taaaacagaa
     3541 agattactat gaaaatgcaa caaaactaca ggcagcctta acatctaaag accaaccaga
     3601 tgtaacatta ttagaaataa ctcaaacagg tacattcgca tcagcaggtg ctttagtaga
     3661 tatgagtaaa tattttgata aaacatatca agaaagattc ttttctggtc ttttaacaaa
     3721 ttcatattat gaggataagt ttgttggtat gccatttaat agaagtacac caatattata
     3781 cataaataaa gatatggcaa ctaaagcagg tcttgaccct tcaggtccaa aatcatggga
     3841 agagcttaag gattatgcta gtaaaatgac aaataaatca gaagatactt atggttttga
     3901 aacaccaata gatatttggt tctatgaagc tatggtaatg caaagtggag gagaaataac
     3961 taatggtaaa aaagtagcat ttaataatga ggcaggacaa gcaccagtta agttctggca
     4021 agatatgatg aaagctggaa taatgaaaat gcctcctgga gaagattata atgcctggga
     4081 cgtagcaaaa caagattttg taaatggtaa agtaggtatg atatttacat caacagcaga
     4141 tttagcagga cttatgcaac aaacagaagg taagtttgaa atatcaacag cattcttacc
     4201 aaaaaatcaa aaatacgcaa ctcctacagg tggagctaat ctagttatgc ttgaaggtgg
     4261 aacagataaa gaaaaagaag caagtgcaga atttatagaa tggatgacac aaacagataa
     4321 aatcgttcag tttagttcat caacaggata cttaccaaca actgaagatg caacaaactc
     4381 agaaaagtta caaacattat ataaagaaaa acctcaatat aaggttgcaa ctgaccaatt
     4441 acaatatgct gttgcacttc caatgttaaa tggatacaag gaagcaactg ataagctaat
     4501 ggatgagatt aaaaaggggc ttactgattt aaatgcaaat ccaaaagatg tagtaagcaa
     4561 aggaacttct gcaatgcaag cagtaataga taaaacaaat aaataattat atagacttgt
     4621 aagttgatta aaagtattca ataatatata aatgaagagg taatgtaaat gacaaaggtt
     4681 atagtattat cagtagattc tttatttgaa aaagacttgg aatttgtaaa aaatctacca
     4741 aactttaaat ctatactaga aaattgctcc atagtaaaaa atataaattg tgtttatccc
     4801 actctgacat atccgtgtca tactagtatg gttacaggag tatatcctaa aaaacatggt
     4861 atatatcata atgaaaaatt tgacccaaat aaagaaaata aagactggta ctggtactca
     4921 aaggacataa aagcaaaaac tattatagat gtagcaaaag aaaataatct aactacctct
     4981 agtgtattat ggcctgtaat gggagcaaat ccaaatatag attataatat tgcagaaata
     5041 tgggctccta gtagagaaga tgacccaaga gaaacttttg aacgaagttc cagtaaggtt
     5101 ataatggacg gtatttacaa tagacattgt cattatatcg attggaaatt agaacccaac
     5161 atggatttat ttggagtaaa ctgttcagta gatataataa aggaatataa accagattta
     5221 atgctaatac atttagctac attggaccat acacgacaca attatggctt atttaatgac
     5281 gaagtagata aagcattaaa aatgaatgat aaatggctag gagatattat tcaggctaca
     5341 aaggatgcag gtacatacaa agatacaaat tttataatac ttggggacca tggtcatctt
     5401 agagttgata aagttataaa tccaaatgtc ttgcttaaat caaatggatt tataaaagtt
     5461 gaaaatggag aagttaagga ttttgatgcc tatgtaaatt cttctggaat atcagctcaa
     5521 ataatagtaa ataacttaga taggttaaca gagttaagag aactgcttga agaatttaaa
     5581 gaagaattat tcatagagaa tatttttacc aaagaggaag catctaacct tggtcttgaa
     5641 ggtgattttg aaatggtaat tgaaggtctt gaaggaattt catttggaaa tgatttcgaa
     5701 ggaagtatta taaaagattc agatataaaa gattacaagt ttgctgttgc gacacatggt
     5761 catttaccta ctaaaggtaa tagacctcca tttatagctt ttggaccaaa tattaaaggt
     5821 ggtgtggtta tagaagaagg agacctgaga gcacatgcag caactatatt aaaaatgttt
     5881 aatttaaaat tagatggagt tgaaaaagaa gcatttgatt ttattatgta agttgaataa
     5941 agtatttatt ttccatgaat tatagacttc tattgcaaag atagaagtct ttttttatgt
     6001 tttagattta agtaaaattt aaatgggtaa aatatttaaa ataacttaaa caattttata
     6061 aaggtatgat ataataatac atgtcaggaa ctaatgttcg aaaaaaggag tgatactatg
     6121 gattttaaaa taaaatcaga cttcaaacct acaggtgacc aaccagaagc tattaaatca
     6181 atagtggatt ctataaatag aaatgagaaa ttttcgacat tattaggtgt aacgggttca
     6241 ggaaaaacat tcacaatggc aaatatcatt caacaagtta agaaacctac cctaataatg
     6301 gctcataata aaactctagc agcacaactt tacagtgagt ttaaagaatt tttcccagac
     6361 aatgcagttg aatactttgt aagttactat gattattatc aaccagaagc atatgtagct
     6421 cacagtgaca cttatataga aaaagatgca agcataaatg atgaaataga taaacttcga
     6481 cattctgcta cagcatctat tttagagaga agagatacta taataatatc atctgtatct
     6541 tgtatatatg gacttggtga cccaaaagac tataaagaac ttatgttatc tataagacca
     6601 ggtatgcaaa gagacagaga tgatgtaata aaaaggttaa tagagataca atacgaaaga
     6661 aatgacataa actttacacg aggtaccttt agagttagag gagatatact agaaatattc
     6721 ccagctagta atgatgaaaa agcaataaga atagaatttt ttggtgatga ggtagataga
     6781 ataacagaaa ttgattatgt aacagggaag attgttggta ctagaaatca tgttgttata
     6841 ttcccagcat ctcactatgt aacaacgcca gaaagaattg aaaaggcaat tgtagaaata
     6901 gaaaacgagc ttcaagaaca aattaaattt ttcaaggaaa atgataggct attagaagca
     6961 caaagaattg aacaaagaac taagtatgat atagaaatgt taaaggaaat aggattttgt
     7021 caggggatag agaactattc tagacatatt acaggaaggt cagaaggaga aagaccatat
     7081 actttaatgg atttcttccc agatgattat ttgataattg tagatgaggc acatgtcact
     7141 ataccacaag taagaggtat gtatgcagga gacaggtcta gaaaaacatc acttatagag
     7201 aatggtttta gattaccttc tgcacttgac aatagaccat taaattttca agaatttgaa
     7261 ggaaatataa atcaaatgtt gtttgtaagt gctacaccag ggccttatga aattcaacac
     7321 tcagagacaa tagcagaaca gataataaga ccaactggcc ttttagaccc aattgtagaa
     7381 gtaagaccta taaataatca aatagatgat ttggtaggag aaataactaa aactatagaa
     7441 aaaaatgaaa gagtgcttat aaccacttta actaaaaaaa tgagtgagga cttgactaac
     7501 tatcttaaag aaatagggat aaaagttaaa tatttacact cagatatagt tacattagaa
     7561 agaacagaaa taataagaga tttaagatta gggaagtttg atgtccttgt aggaataaac
     7621 ttacttagag aaggattgga tataccagaa gtttcactta tagcaatttt agatgcagac
     7681 aaagaaggtt tcttacgttc cgaaacagct ttaatacaga ctattggacg tgcagctaga
     7741 aatgaaaatg gaagagtaat tatgtatgca gacaggataa ctgactcaat gcaaaatgct
     7801 attgatgaaa caaagagaag aagagatata caaaatttat ataatgaaga gcacaatatc
     7861 attcctaaaa ctatacagaa aaatataaga gatagtatag aagctactaa ggttgcagag
     7921 gaagaagttg tatatggaat atctgataca gatgataaag acgaaataag agctaatata
     7981 gataaattaa aatcagagat gatggaagct gctcaaaact tacaatttga aagagcagca
     8041 gaacttagag ataaagtgaa acaattagaa gaaaaattgg aaaaatagaa aggtaaaaac
     8101 aacattatgg aagataaaat tataataaga ggagcaaaag aacataattt aaaaaatata
     8161 gatttagaat taccaagaga taaatttatt gtttttacag gtttgtcagg gtctgggaag
     8221 tcttctttgg catttgatac aatatatgca gaaggacaga gaagatatgt agaaagtcta
     8281 tctgcttatg caagacaatt tttaggtcag atggaaaagc caaatgtaga atatatagag
     8341 ggtttatcac cagctatctc aattgaccag aagactacct caaaaaaccc acgttctacg
     8401 gttggaacag taacagaaat atatgattat ttgagactat tatttgcaag agtaggagat
     8461 gtgtattgcc ctacatgtgg tgaaaaaata agtcagatga ctattcaaga aatagttgat
     8521 aaaatgctag attttccaga tagaacgaaa ttacaaattc tttcaccaat tgttagagga
     8581 caaaaaggta cacataaaaa agtctttgat aatatcaaaa aagaaggatt tgtaagagtt
     8641 agagttaatg gtgaaaacta tgaagttagt gatgatataa tcttaaataa aaatcaaaaa
     8701 cataatatag aagttgtagt ggacagaata gtaattaaag atggaataga atcaagacta
     8761 gcagattcaa ttgaaactgc agttaagcta tctgatggtc ttgtaatagc ccaaataata
     8821 gatggagaag aaattctttt ttcaactaaa tttgcatgcc cagaacatgg aattggcata
     8881 gaagaattat ctccaagaat gttttcattt aatgctccct ttggtgcttg tgatgtttgt
     8941 aatggtcttg gggaaagtaa agaagttgac ccagaattag tagtaccaaa caaagattta
     9001 tcaataaaac aaggtgcagt ggcagcatgg ggttcaacag gtgttaatga tgacacttat
     9061 tacagtaaaa tgataaaaag tttagcagag cattttaatg tatctttgac aacaccgttt
     9121 aaagatttac cagatgattt tgtggaggaa ttactttatg gtaaagataa tataattgta
     9181 gagtttactt atgagtcaaa atttggagga actagaaatt ataaatcata ctttgaaggt
     9241 gtaatagtaa acttagagag aagatataga gaaactaatt cagattacat gagagataag
     9301 attgaagaat acatggcaga gagaccatgt ccaaagtgta aagggatgag acttaaaaag
     9361 gaagttttat ctgttttagt tgatggaaaa aatataatgg aagttacaaa cttatctgta
     9421 aatgaattga ttgactttat ggaaaatata aatcttacag aaaaacagag atttatagct
     9481 catgagatta taaaagagat aaaaggaaga gctatgttct taagagatgt aggtcttgat
     9541 tatttaaatt tatcaagaaa agctggaact ttatcaggag gagaagcgca acgtataaga
     9601 ttagctactc aaataggttc tgcattggtt ggagttttat atgtacttga tgagccaagt
     9661 atagggcttc accaaagaga taatgatagg cttataacta ctcttagaca tctaacagat
     9721 ttaggaaata ccttaatcgt tgtagagcat gatgaagaca caatgagaga agctgattat
     9781 gttatagaca ttggtccagg tgcaggtata catggtggtg aaatagttgc acagggtact
     9841 ttagatgaaa taattgaaaa tgagaactca ataacgggtc aatatttaag tggtaaaaaa
     9901 caaatattgc ttcctgagac tactagagaa ggaaatggaa gttttataga aatagttaaa
     9961 gcatcagaaa ataatctgaa aaatattgat gtaaagttcc cattaggtaa gtttacatgc
    10021 ataacaggtg tttcaggttc tggaaaaagt acattaataa acgatatact ttacaaaggt
    10081 gttgcaagta aagtgaatag acttaagcaa agagctggta agcataagga gataaaagga
    10141 attgaaagta tagataaagt tataaatata gaccaaagtc ctattggaag aacacctcgt
    10201 tcaaatcctg ctacttatac aggtgtattt gattttataa gagatatttt tgcatctact
    10261 aatgaagcta aagcaagagg ttataaaaaa ggtagattta gttttaatgt aaaaggtggt
    10321 agatgtgaag cttgtaaggg tgatggaata ataaaaatag aaatgcattt cttaccagat
    10381 gtatatgttc cttgtgaaat ttgtaaaggt gaaagataca atagagaaac attgcaagta
    10441 aaatacaaag ataaaactat atctgatata ttagatatga atgtggaaga agctgttgag
    10501 tttttcgaaa atataccaaa tataaaaaga aaattagaaa ctttaatgga tgtaggactt
    10561 tcatatatta aattaggaca gccttcaact caattatcag gaggagaagc acaacgtata
    10621 aaattagctg ctgaacttag taaaagacca acaggaaaga ctttgtatat attagatgaa
    10681 cctactactg gacttcatat ggcagatgta gacaagctaa tccaagtatt acaaagacta
    10741 gcagatacag gaaatacaat tatagtcata gaacataatt tagatgttat aaagacttgt
    10801 gattatgtaa tagacttagg accagaaggc ggagataagg gtggaactat agttgcaact
    10861 ggaacaccaa aagaagtatc taaagttgat ggttcttata caggtcaatt cttaaagaaa
    10921 tatttttctt aagttatata aattgtgaaa aattttagag cagatatacg ataaactgtt
    10981 ttgttataag taaattagtc aaatataagt tgtaaatagt aatacaaagt aaagtccata
    11041 ttactcttca tggttaacat tgttttcaaa tcaattatag aagggaatat gggctttaac
    11101 ttgtttaaag cactggaaat tgtataactt tttaaaattc atattttagg gtataatttt
    11161 agtataaaat ttaatgtttt ggagggcgag atgtttgata tacaagagca tttaaaaaaa
    11221 ttaccttctg agcccggagt atatttaatg aaagacaaat acgaccatat aatatatgtg
    11281 ggaaaagcca tatctctaaa aaatagggta aggcagtatt ttcaatcttc aaaaaaccat
    11341 acatctaaag taaaatctat ggttaagaat atatataagt ttgagtatat tataacggat
    11401 tcagagttag aggcattaat acttgaatgt aatttaataa aaagatacag accaaagtat
    11461 aatgtagtgc ttagagatga taaaacttat ccatatataa aagtaactac gaatgaagat
    11521 tatccaagaa tcttaaaagt tagaagagtt cttaaagata aggcaaagta ttttgggccc
    11581 tataccaata taactgctgt gaatgatacc ttggagctta taagtagtac gtatccaata
    11641 agaagctgta aaatagatat agataaagca ataaagaata agacaagacc ttgtttaaat
    11701 ctacacataa ataaatgttt aggaccttgt acaggaaatg tttctaaaga agagtatgga
    11761 aaaatgatag aagagattat aatgtgttta tctggtaaag aagaaaaatt gatggaactt
    11821 ttaaaggaaa aaatgaatga gagttctatg aactttagat ttgaagaggc tgctgtatat
    11881 agggataaga taaagagcct tgaagagatg atacagaaac aaaaaataga tgctactgta
    11941 agtgacttaa accaagatgt ggttgcaatg gcaagagctc ataatgaagc ttgtgtccag
    12001 gtatttttta taagaaatgg taagattgta ggtagggaac attttatatt agaaggggta
    12061 atggatagtc caagagcttc tatattaagt tcatttgtaa aacaatttta taatgaacaa
    12121 gagtatatac ctaaagaatt aattatagaa gatgagatag aagatagttc tatattagaa
    12181 gaatggctgt catctaaaaa ggggcaaaaa gtaacaataa gagttcctca aaagggagag
    12241 aagaaaagct tagttgaaat ggttaggaaa aatgctgtag aatatcttga aaaattttct
    12301 gatatgaata aaagaaaata tgaaaaaagt attggagcat tggaagaatt aaagcaaata
    12361 ttaaatctag aaaagttgcc aataagaata gaggcttatg atatatcaaa tatacaaggt
    12421 gtagattcga ttggttctat ggtagtgtat acgaatgcta aaaaagataa gaaggaatat
    12481 aggagatata aaataaagac agttattgga cctaatgatt atgattctat ggctgaaatt
    12541 gtagatagaa gattaaaaca tggaaattta cctgacttga tattgttgga tggtggaaaa
    12601 ggacaagtta gtgcagtaaa aaaagtacta gaactcaatg atgtagatat acctctttgg
    12661 ggaatgtaca aagatgataa gcatagaact aaaggattga tatgcaaaga aaaagaaata
    12721 gaactagata aaacaactaa tttatataga tttattgcta gtatacaaga agaagttcat
    12781 aattatgcaa taacatatca tagaagtcta agaaataaag ctttgacaaa atctatctta
    12841 gatgatatac aaggaatagg tgaaaaaaga aagaaatcat tattaaatca ctttaaagat
    12901 gtagatgcta taaaaaaagc tactatggaa gaacttttag aagtagatgg aatgaataaa
    12961 agcattgcag ataatgttta taattttttt agaaaggaag aaaattaatg gaaactagca
    13021 ataaagtatc aataagagag ataataaaag agttgaagtt aaaagtagta tacatgcctg
    13081 atgatgtaga ttattatgtc tactctcaag atataaatag accaggatta cagtttgcag
    13141 gatattttga ttattttgca tatgaaagaa tacaaatagt tggaaagaca gaatataatt
    13201 tttttggatt aatggatagt catattcggg aagaagtact agatagattt ttctcatatg
    13261 aaataccagc gttaataatt tctagagact tggaaatcaa gccagatgta attgaaaagg
    13321 ctaaaaaata taaaagaata cttttgagca caaatagaaa taccactaga cttataaata
    13381 gactgtcaaa ctatttggat aataagttag caccacatac aacaattcac ggtgtgctag
    13441 tagatattta tggtataggt gtactgataa aaggtgaaag tagtatagga aagtcggaaa
    13501 cagcattaga actgattcaa aggggtaata gattagttgc agatgatgct gtagaaataa
    13561 gaaaattaga tgaaagtatg cttgtgggtc agtcacctga actaataaga cattttcttg
    13621 agataagagg aataggaata atagatgtta gaagtttata tggagtaggg gcaatcaaaa
    13681 attctaaaat gatagattta gtagttcatt tagaagcttg ggatgaaact aagtattatg
    13741 atagactagg actggataaa gaatatgaag aaatacttgg tgttaatgta gagaaattag
    13801 ttgtacctgt taaacctgga agaaatactg ctatgatatt agaagtagca gcaatgaatt
    13861 ttagacaaag aggaatggga tacgatgcag ctcaagaatt tactaaaaaa ttatcaaaat
    13921 taatcgatac taagtagtac tgaataatag tggactacac atcatttaat taaatattga
    13981 ttaggattat gtcctacacc ttataaatta aaatcaagtt gtatatataa tgtttattaa
    14041 taagatatat tatataagga taatttaaag tacagtgtta gattataata atactagatt
    14101 aattttatat ttgagtttca ttatataaca agaaagttgg aattttttat gggaatagaa
    14161 tattttagta aacttttaga aagtagtact gaaaaagaat tgctcttaag taaaaagtca
    14221 aataacataa gtacagcaag attaatatct tttttgatag tgatagcagg gcttgctatt
    14281 ggattttata ataaaaatat agtaggtatt tttgttggag ttttatcaat aataatattt
    14341 atagcactct tagttattca tagtaaagta aaagaagaag aaacatattt taaaagtaag
    14401 agcgaagtat taaataagta tattaagaga tttggagatg gttggaagga atttaaaata
    14461 gatggtaaag aatacttaaa agatgaaaat tcgcaagcaa aagatttaga cttatttgga
    14521 agagcatcat tgtatcaata tatctgtgta gctaatacat cttatgggaa aaatttttta
    14581 gccaaatatt tgtggaatga aaatccagat gagaatataa ttttagaaag acagagagct
    14641 ataaaagaac ttttatcaaa agatgatttt tctattcata ttcaaacact cagtaatata
    14701 attggtaaag agcaaaaaaa caattcagat agtagtatag aatcgtttat agaatatgga
    14761 gaaaataaga atgtgtatat accaaaatgg atgcatattt ttacttgggg tttaccaact
    14821 gcaacaatct tatcttttat attttgtatg ttaggatttt tacctgtttt acctgttttt
    14881 ttacttttta taatccagtt agggtttagt ggttttggat atccaagact tatgcaaact
    14941 cttgctccat tattttcatt tagtagaagt atacaagtat atgaaaaaat gtttgaggtt
    15001 ttagaaaaag agacgtttga gagtgcatat ttgaaagaat tacaagataa actttctaag
    15061 ggaagtggag tttcaagagg tgttaaacaa ttaaattcaa taggaaatgc tgtaaaccta
    15121 aggtataatc agatactata tataattgcc tgtggtgttt tgatgtggaa ctatcattgt
    15181 gcagaagctt tggagcgatg gaaaggtata tatggaaatc aaattagaga ttggtttgaa
    15241 tctattggag aatttgaagc tctaataagt ttaacagttg tttctcatgt aaaggaaaat
    15301 acatgttttc cagtaataaa atatgaagat actccaagat tgaaagttga agaggtatac
    15361 cacccattaa tagcagaaaa aagtgtcata gcaaattcga tacaattaaa ttctcagact
    15421 tgtattatca caggttcaaa tatgtctgga aagacaacat ttttaagaag tatcggtgta
    15481 aacttagtgc tggcatatgc aggtgctcca gtatgcgcta agagttttga tgcgacctgt
    15541 atggctatat ttacttctat gagaatacaa gatgatgtaa gtcaaggaat atcaactttc
    15601 tatgctgaga ttcttagaat aaaatcaatg attcaatata gtttaaaaga actgccaatg
    15661 ttggttctag tagatgaaat ttttaaggga actaactctg ctgatagaat tattggagca
    15721 agtgaggcag taaagaaatt atcaaaaccc tgggttataa gtatggtgac aacacatgac
    15781 tttgagctat gtgacctttc aggtagcgga gatgttgaag ttgtaaacta tcatttctca
    15841 gagtactatg tagacgataa aattcatttt gactataaga taaaagatgg aagatgtaaa
    15901 actacaaatg caaaacaact tatgagaatg gcaggtattc tttaattaat agataggtta
    15961 attaataaat aagtctattt agaaatataa tttaatttgt gaaattaata aggattgtct
    16021 taaattacgt aaaagtattt taagacaatc cttttttaag aataaatttt atactttgtt
    16081 atgactactt agcttcttca caagcacatt cttgtggaca ctctccatag acacaactct
    16141 tatcaaagta atgagtatga aggtatttat gagctacatc actatttggt tctccaaaga
    16201 agtctttata aatagcttgt aaatcaggat tctcatgaga tttacgaata gccttatttc
    16261 tatctatttc atataacgca tttgcacgtc tatcaactat ttcagtatta ccatggtgat
    16321 aaggttgacc agcacctcca acacatccac caggacatgc cataacttct atgatatggt
    16381 atttacattc tccacttctg accttatcca taatctttct agcgttttta agtgtattta
    16441 caatacatac attaactgta gttccatcaa catctatact tgcttcccta attcctttaa
    16501 gacctctaac attagtgaag tttacattat ctaaagtctt attagttatc ttctcatatg
    16561 aagtacgtag ggcagcttct aaaactccac cacttgctcc aaatatagat gcagcacctg
    16621 tagattttcc tagaggatta tcaaaatctt ggtcttgaag attaggtaaa tcgattgcag
    16681 cttcttttat catcttagct aactctctag tagttataga aaggtcaaca tctagtattc
    16741 cagattcact taattcttct cttgaagctt catatttttt agctacacaa ggcattacag
    16801 atacaacata aagttcttca ggattgattc ctaatatttt aggtgcatag tagtttttag
    16861 ctatagaacc aaacatacct tgaggagact tacagctaga tgctaagttt aacttgtctg
    16921 gataattgtg ctctaagaag tttacccatg caggacaaca acttgttaat ataggaagat
    16981 tttcaccttt ttggattctt tctataaatt cagtagcttc ttccattata gtaaaatctg
    17041 caccaaagtt tgtatcaaat acatgttcaa atccaagtgc ttttaaagca gctaccattt
    17101 tccctgtaga gattgaccct ggttctaatc caaactcttc accaatagca actcttacag
    17161 caggagcaac ttgcactact atagtctttt cttttttatt taaaacatcc caaagtttag
    17221 gtacattatc tacttctgtt aatgctccag ttggacagac agatatacat tgaccacaga
    17281 atgtacactc tgtttcaacc atatcagagt taaagaatgt actaactaaa gtattaaatc
    17341 cacgattaac tccagataat gctcctactg tttgtatgtc attacataca gtctcacatc
    17401 ttctacataa tatacactta gaatggtctt taactaaaga tttagtagaa gtatctttac
    17461 ctgcaaaaga tttagaacct tgatatctta tttttcttac tcctaaatca gcagctatag
    17521 tctgtaattc acaatctcca tttttttcac agacaaaaca atcttgtgga tggtctgata
    17581 acaataattc aacaatagtt ctacgagcat ttaatgcttt agcactatta gtctgaactt
    17641 tcataccttc ctttataact gttccacaag ctggaactaa tcctctctct gtctcaacca
    17701 tacaaacacg acaagaagca caagtgtcaa gcttatctat ttcattcata tgtaaatgac
    17761 aaaggttgtg tatttttata tttattaatt tcgcagcatc taagatagaa gttccactag
    17821 gtgctgaaac gtgtttgcca ttaatagtta aattaactaa actcattgtt attcctcctc
    17881 caaataagtt atttcttgat tatagcgtta aatgtacaat tatctataca agcaccacat
    17941 tttagacact tagaagtatc tattgtatgt tgttccttaa cagaacctgt tatacaacca
    18001 gcaggacaca ctctagaaca ttttgtacaa cccttacagt catctgttat aaagtaagaa
    18061 agtaagttct gacatttacc agcaggacat ttcttatcta caacatgagc tatatactca
    18121 tctttgaaat attgaagagt acttaaaaca gggtttggag cagctttacc aagaccacat
    18181 aatgaagcag tttgtatagt ttcagcaagg ttttctaggt catctaaatc ttgtagagta
    18241 ccattacctt ctgtaatttt agttaatatt tctaataatc ttgtagtacc tatacgacaa
    18301 ggtgtacatt taccacaaga ttcttcaaca gtaaactcaa ggaagaactt ggctatatct
    18361 accatacagt cagattcatc aagaacaagc ataccaccag agcccatcat tgaaccgata
    18421 gaacttaatg aaccaaagtc tataggagtg tctaaatgtt cagttggtat acatccacca
    18481 gatggtccac ctgtttgaac agccttaaat ttcttacctt cagggattcc cccaccaatt
    18541 tcaaatacaa tatctcttaa agtagtaccc attggaactt caacaagtcc tacattttct
    18601 acctttccac caagagcaaa gactttagta ccactagaat cttcagtacc taagttggca
    18661 aaccaatctc cacccttaag tataattgca ggtatgttag caaaagtctc aacgttattt
    18721 aagcaagtag gagatttcca tagaccactc ttagaagaac tataagtttt cattctaggt
    18781 tcaccacgtc taccttcaat tgaatgcatt agcgctgtac cttcaccaca aacgaaagca
    18841 cctgcaccat attttaattc taatttaaag ttaaatccag tgcctaagat attttcgcca
    18901 agtaaaccgt atttttcagc ttgagctatg gctactttta atctttctat agattttgga
    18961 tattctgctc ttatgtatat gtatcctgta tcagaaccta tagcgtatcc acatattgcc
    19021 atagcttcta gtacactatg agggtcacct tcaagtacag atctatccat aaatgcacct
    19081 gggtcacctt catcagcatt acatacaaca aactttttat ctgttggacc agctggattt
    19141 tttgaagcag cttcccattt agagccagta gggaaacctg caccacctct accacgaagg
    19201 ccagaagttt ttacctctgc aataacttct tgaggagtaa gagaagttaa acatttgcct
    19261 agagctaaat aaccatcatt ggcaatatat tcttctatac tatctgggtc tataagacca
    19321 caatttttta aagctatacg taactgtttt ttgtagaaag acatttcttc ttgttcttca
    19381 acttttttac ttaaagattc ttcctcatat aataattcct cgacaactgt atttctaatt
    19441 aagtggcttt gaactatctt ttcagcatca gatggctcaa cttttacgta gaaaacgtta
    19501 tcaggataaa cttttacaat aggaccttga gcacagaaac caaaacaacc tgtcaaacga
    19561 acactaactt tatcttggat tcctgatttc tttatttcat tttctaattc actaactatt
    19621 tctagactgt ttgaagatgt acatccagta tcacaacaaa caagtaattc tcttcttaat
    19681 acattttcct tagaataatt ttttcttaag ctaagattag gtttcagctc atcagctaaa
    19741 acttttaatt catcaaatga atttacttta cgcattattt attcacccct cttaacaatt
    19801 caattctaat tctctataag tatctaatat ttcgcttact tggtcaggtt tcactttacc
    19861 ataaacttta ccatttacag taacaactgg agctaaacca catgccccca aacatcttaa
    19921 accttctaaa gaaaatttaa aatcactagt agtttctcca acttttatgc caagctgttt
    19981 ttcaaaagca cttaagattt tgtcagcacc ttttacaaaa catacagttc ctagacagac
    20041 actgatttta tacttaccta caggttctgt agtaaaataa gaatagaatg taacaacccc
    20101 atataccttt gcaggagcta ctccaagttt tctagcaaca tgaagttgaa cttcttttgg
    20161 aagatatcca aatatacctt gggcttcatg tagaacttgt attaacgcac cttcctttgt
    20221 tgcaagagag tcaataaata tatctagttc gtcaaataat tgcttgttgt gtgaaataaa
    20281 atcgcacata acaaatcccc cttttctttt taaagttatt atttttcata aaataattaa
    20341 ccatgagttg accccaacat tatgtgggtg aacaaaacca aaacaataaa gaaagaataa
    20401 tatacaacaa tttctttatt tataaaaaca atgcaaaaaa gttacctatt aaaaataaaa
    20461 actatactag tctattataa tataaaataa ttcagaatag ttaaaaaaaa taaaaaaaat
    20521 gagctaaatt caataaaaaa aaaataatac atataaaaaa ctttaaaaaa taaaccaaaa
    20581 tgtaaaattt ttataaaata aaagatttat aaaactgctg ataatagtct tgaaattgat
    20641 tccttgattt ttatataagt agacctagaa ttatatatat ctaatgttat ttccttagaa
    20701 tttaaaatat cattttcaaa tataattctt tgttccaatg ctttttttga agagtacata
    20761 aatgcattaa cttcgaaatt aagctcaaaa cttctgatat ccatattagc tgttcctata
    20821 gagcatatag agtcatctat aactattgtc ttagcatgta aaaatgcatt ttgaccataa
    20881 gtatagattt tagctccaaa ctttaaaagt tctccagcat atgaatatga tgcccagtat
    20941 acaaagggat ggtcaggttt agaaggaatc atcactctca catcaacacc agataaacag
    21001 gctattttta aagtatctat aaaagttgag tcaagtatta aataaggact ctgtatgtat
    21061 atatactttc tagctttctg aatcatttta aggtaaccat atttaatctc atcaagttct
    21121 gatatatctg gaccacttga tactatctga atcccaatat tttcagaagg atgattagaa
    21181 tttgtatctt cttctataaa gtatttttct aaatctaagt cttctttagt tgtatatctc
    21241 caatcaagga tgaatctcat atttaggtca attacgcaat caccagtaag cttagtatgt
    21301 gtatctctcc atagaccaaa ttttggattt ctacctaaat attcatcacc aacattatat
    21361 ccacctacaa atcctacctt accatcaatg acaacaattt ttcggtgatt tctatagttt
    21421 aagttaaagt ttacaatttt caagaatgat gggaagaagc ttccaacttt aacaccactt
    21481 tctttaagtc tggaaagtgt actatttttt agtgttctac caccaactgc atcaaataaa
    21541 agtcttactt caacaccttc tttagctttg tctataagaa tgtctattat ttcagttccg
    21601 attttgtcat ctttgaaaat gtaaaattgt atgtttatat attttttagc ttttttaagt
    21661 tcttcaagaa gactgttaaa aaattgacta gattcagcat aaatccacac atcgttatta
    21721 ttggtataat gagcattatt tgaattagcc aatgcataaa tcatatcctt atgttgatgt
    21781 atatctgaat caatttcaga tgtagattgg agtttaacct gagtatctag tatattactt
    21841 ttgattatct tatcatcctt ttctttcaat ctaaacatat tgttttttga aatatttctg
    21901 ccaaaagcta tataaagtat gaatccaaga gcaggaatca atactaatat aagaagccat
    21961 gttaaagttg tatttatgtt tcttttttct ctaaagataa ggtttagtat aaccatgaaa
    22021 ttaattatat atattgtagt aactgctatt tcatatattg acatttcgaa aaatgatata
    22081 tcaaacataa aattccctcc ttcaaatatt tattaaaata agtaattata ta
//
LOCUS       DAYAPA010000063        21942 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.063, whole genome shotgun sequence.
ACCESSION   DAYAPA010000063 DAYAPA010000000
VERSION     DAYAPA010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21942
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.063"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            370..681
                     /gene="rpsJ"
                     /locus_tag="ACGI59_002164"
     CDS             370..681
                     /gene="rpsJ"
                     /locus_tag="ACGI59_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFG9739950.1"
                     /translation="MAKNEKIRIRLKSYDHKLLDFSAGKIVETAKKAGSQVSGPVPLP
                     TEKQVVTILRAVHKYKDSREQFEIRTHKRLIDIANPTPKTVDSLMRLDLPAGVDIEIK
                     L"
     gene            773..1402
                     /gene="rplC"
                     /locus_tag="ACGI59_002165"
     CDS             773..1402
                     /gene="rplC"
                     /locus_tag="ACGI59_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFG9739951.1"
                     /translation="MKGILGKKVGMTQIFTDKGVVIPVTAVEAGPMVVTQIKTVDKDG
                     YNAIQIGFEDAKEKALNKPKKGHLAAANVLKKHLKEFRVDSVEGYTVGQEIKADVFEA
                     GAKIDVTGISKGKGFQGPIKRHGQSRGPETHGSRYHRRPGSMGACSYPGRVFKNKKLA
                     GHMGSVKVTVQNLEVVKVDADKNLILVKGAIPGAKGSVVTIKEAIKVSK"
     gene            1432..2055
                     /gene="rplD"
                     /locus_tag="ACGI59_002166"
     CDS             1432..2055
                     /gene="rplD"
                     /locus_tag="ACGI59_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFG9739952.1"
                     /translation="MPKLNVLNVSGQNVGEIELSDSIFGVEVNGHVLYEVVKNQLANK
                     RQGTQSAKTRAEVRGGGRKPWKQKGTGRARQGSTRSVQWVGGGVAFAPKPRSYKYTLP
                     KKVRRLAMKSALSSKVQNSEVIVLDALNMDAPKTKEFAQILNNINAAKKALVVIADKN
                     DNVIKSARNIEGVQTALVNTMNVYDILKYDSFIITTDAVKKVEEVYA"
     gene            2055..2345
                     /gene="rplW"
                     /locus_tag="ACGI59_002167"
     CDS             2055..2345
                     /gene="rplW"
                     /locus_tag="ACGI59_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFG9739953.1"
                     /translation="MTNPHDVIIRPVVTEHSMAEMGEKKYTFVVAKDANKTEIKKAVE
                     KVFGVSVDKVNTLNYDGKVKRMGRTQGRTSSFKKAVVKLTADSKEIEFFQGM"
     gene            2375..3205
                     /gene="rplB"
                     /locus_tag="ACGI59_002168"
     CDS             2375..3205
                     /gene="rplB"
                     /locus_tag="ACGI59_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFG9739954.1"
                     /translation="MAIKKFRPTSPALRQMTVLVSDEITCNQPEKSLLVNLKKNAGRN
                     VHGRITVRHRGGGQKRKYRIIDFKRDKDGIPAKVATIEYDPNRTANIALLNYADGEKR
                     YILAPVGINVGDTILSGLGADIKPGNCLALKDMPVGTIIHNIELKPGKGAQLVRSAGV
                     SAQLMAKEGKNALLRLPSGEMRLVSINCKATIGQVGNIEHGNVVIGKAGRKRHMGIRP
                     TVRGSVMNPNDHPHGGGEGRSPIGRPSPVTPWGKPALGYKTRKKNKASNKLIVSRRTK
                     "
     gene            3240..3521
                     /gene="rpsS"
                     /locus_tag="ACGI59_002169"
     CDS             3240..3521
                     /gene="rpsS"
                     /locus_tag="ACGI59_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFG9739955.1"
                     /translation="MSRSTKKGPFVHARLLKKIEAMNASGNKEVIKTWSRSSTVFPQM
                     VENTIAVHDGRKHVPVYITEDMVGHKLGEFVPTRTFKGHKDDEKSNKRK"
     gene            3553..3888
                     /gene="rplV"
                     /locus_tag="ACGI59_002170"
     CDS             3553..3888
                     /gene="rplV"
                     /locus_tag="ACGI59_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFG9739956.1"
                     /translation="MEAKATAKYVRVSPRKAGQICDLVRGKNVDEALAILKFTPRGAA
                     SIIAKVVKSAKANAENNHEMDTEKLYIASIVANQGPTMKRFMPRAMGRATTIRKRTSH
                     IEVVVKEKK"
     gene            3911..4726
                     /gene="rpsC"
                     /locus_tag="ACGI59_002171"
     CDS             3911..4726
                     /gene="rpsC"
                     /locus_tag="ACGI59_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFG9739957.1"
                     /translation="MGQKVNPHGLRVGVIKDWDSRWFATDKKEFGNLLLEDHNIRKFL
                     KKRLYSAGVAKIEIERSANKIKMDLHVAKPGVVIGRAGAGIEALKAELEKMTKKTIIV
                     NIVEVRSTDKNAQLVAENIALAIERRVAFRRAMKQAIQRAMKSGAKGIKVSASGRLGG
                     AEMARTEGYSEGNVPLQTLRADIDYGFAEADTTYGKIGIKVWICNGEVLPTRDGVNPR
                     EESRKSDRRDNKRDNRRNDRRGNDRRGNDNRGNYRGQRPQGGSRPQRTENKGN"
     gene            4762..5193
                     /gene="rplP"
                     /locus_tag="ACGI59_002172"
     CDS             4762..5193
                     /gene="rplP"
                     /locus_tag="ACGI59_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFG9739958.1"
                     /translation="MLMPKRVKRRRVHRGSMAGQAHKGNKVTYGEFGLVALEASWITS
                     NQIEAARIAMTRYIKRGGKVWIKIFPHKPVTRKPAETRMGAGKGSPEYWVAVVKPGRV
                     MFELAGVSEDKAREAMRLAAHKLPIKCKFVKKEDLEVKGGE"
     gene            5195..5398
                     /gene="rpmC"
                     /locus_tag="ACGI59_002173"
     CDS             5195..5398
                     /gene="rpmC"
                     /locus_tag="ACGI59_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFG9739959.1"
                     /translation="MKAKELRDLTSEELMNKLNDFKSELFSLRFQLATGQLENTARIK
                     FVKKDIAKVKTVLAERKLYETRA"
     gene            5422..5676
                     /gene="rpsQ"
                     /locus_tag="ACGI59_002174"
     CDS             5422..5676
                     /gene="rpsQ"
                     /locus_tag="ACGI59_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFG9739960.1"
                     /translation="MERGRRKVRIGRVVSDKMDKTIVVAVEEFVRHPLYNKRVKRTKK
                     FKAHDEKNICNIGDRVKIMETRPLSKDKRFRLVEVVEKVK"
     gene            5702..6070
                     /gene="rplN"
                     /locus_tag="ACGI59_002175"
     CDS             5702..6070
                     /gene="rplN"
                     /locus_tag="ACGI59_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFG9739961.1"
                     /translation="MIQQESRLRVADNSGAKELLCIRVLGGSKRRYGNIGDVIVATVK
                     SATPGGVVKKGKVVKAVIVRSKQGVRRNDGSYISFDENAAVIIKDDKTPVGTRIFGPV
                     ARELRDNEFMKIVSLAPEVL"
     gene            6094..6399
                     /gene="rplX"
                     /locus_tag="ACGI59_002176"
     CDS             6094..6399
                     /gene="rplX"
                     /locus_tag="ACGI59_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFG9739962.1"
                     /translation="MRVKKGDTVVVIAGKDKGKKGSVLKVYPKTSKVLVEGVNVITKH
                     QKPSAMNQQGGIINKEAPIHISNVMPFDPETGKGVRVRYEVKDGNKVRVSAKSGKEL"
     gene            6429..6971
                     /gene="rplE"
                     /locus_tag="ACGI59_002177"
     CDS             6429..6971
                     /gene="rplE"
                     /locus_tag="ACGI59_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFG9739963.1"
                     /translation="MASRLQEKYMKEVAPALMEKFGYKNVMEIPKLNKIVINMGIGDA
                     RENPKGLEKGVEELEMISGQKPVITKARKSVANFKLREGMPIGTKVTLRADKMFYFMD
                     KLVSVSLPRVRDFRGVNPNAFDGRGNYALGVKEQLIFPEIEYDKIDKVRGMDIIFVTT
                     AKTDEEARELLKLLGMPFSK"
     gene            6989..7174
                     /locus_tag="ACGI59_002178"
     CDS             6989..7174
                     /locus_tag="ACGI59_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HFG9739964.1"
                     /translation="MARKAMVVKQQRKQKYATREYTRCTICGRPHSVLKKFGICRICF
                     RELAYKGQIPGVRKASW"
     gene            7207..7605
                     /gene="rpsH"
                     /locus_tag="ACGI59_002179"
     CDS             7207..7605
                     /gene="rpsH"
                     /locus_tag="ACGI59_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003241998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFG9739965.1"
                     /translation="MTMTDPIADMLTRIRNANVVKHETVDVPASNMKKELARILLEEG
                     FIRGYDVIEDGKQGIIRIQLKYGQEGERVITGLKKISKPGMRVYAANHEIPKVLNGLG
                     ISVISTSKGILTDKQARKENVGGEVICYVW"
     gene            7636..8178
                     /gene="rplF"
                     /locus_tag="ACGI59_002180"
     CDS             7636..8178
                     /gene="rplF"
                     /locus_tag="ACGI59_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFG9739966.1"
                     /translation="MSRIGVKPIIIPAGVEVTIAEGNLVTVKGPKGTLTKQLSAELNI
                     KKEENTIMVERPTDNKKHRSLHGLTRTLLDNMVVGVNTGFEKKLELKGVGYRAQKQGK
                     KLVMNLGFSHPVEMEDPEGITVEAPNQTELIVKGIDKQLVGNYAAKIRAWRKPEPYKG
                     KGIKYVDEVIRRKEGKTGKK"
     gene            8214..8582
                     /gene="rplR"
                     /locus_tag="ACGI59_002181"
     CDS             8214..8582
                     /gene="rplR"
                     /locus_tag="ACGI59_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFG9739967.1"
                     /translation="MLKKADKNANRLQRHKRVRRKISGTSQRPRLCVFRSANNIYAQI
                     IDDTKRVTLVAASSLEAEVKSAVNHTGNKEAAKKVGELVAKKAVEKGITEVVFDRGGY
                     LYHGRIQELAEGAREAGLKF"
     gene            8603..9112
                     /gene="rpsE"
                     /locus_tag="ACGI59_002182"
     CDS             8603..9112
                     /gene="rpsE"
                     /locus_tag="ACGI59_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFG9739968.1"
                     /translation="MLRRKPIDAGQLDLQEKVVEVRRVTKVVKGGRNFRFAALVVVGD
                     ENGHVGIGAGKAMEVPDAIKKAVEDAKKNLIVVPIVGTTIPHEVRGHFGAGNILIMPA
                     VEGTGVIAGGPARAVLELAGLKDVRAKSLGSNNPRNMVNATIEGLNSLKTVEDIAKLR
                     GKKVEELLG"
     gene            9127..9312
                     /gene="rpmD"
                     /locus_tag="ACGI59_002183"
     CDS             9127..9312
                     /gene="rpmD"
                     /locus_tag="ACGI59_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFG9739969.1"
                     /translation="MAKLQIKLVRSVIGTTPNQKKNVEALGLRKREQVVVKEDNAQTR
                     GMINKVSHLLEVTEIAE"
     gene            9345..9788
                     /gene="rplO"
                     /locus_tag="ACGI59_002184"
     CDS             9345..9788
                     /gene="rplO"
                     /locus_tag="ACGI59_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFG9739970.1"
                     /translation="MKLHELKPAEGAVRAKRRLGRGTATGQGKTAGRGQKGQWSRSGG
                     GVRVGFEGGQMPLARRLPKRGFNNIFKKVYTEVNVEVLNRFENGTEITAELLKSTKTI
                     SKIGKDGIKILGEGNLEKALTVKAAKFTASAQEKIEKAGGKAELV"
     gene            9832..11100
                     /gene="secY"
                     /locus_tag="ACGI59_002185"
     CDS             9832..11100
                     /gene="secY"
                     /locus_tag="ACGI59_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFG9739971.1"
                     /translation="MLSKLKQAWKIKAVRKKVMYTLMMIVIFRIGTTIPVPGIDTSII
                     QKMVGGNSLLSLYNMFTGGAFSNFSLFALGISPYITASIIIQLLTVGFESLAELQKSG
                     EEGKKKINKYTKYTALALAVVQALGITLGIVRSALISNSVFFITTVVITLVSASMLVM
                     WIGDKITEKGIGNGSSVIIFAGIISRIPTDVIKISQQVKSGEVAPWVIVILAVVILLT
                     VTGVTFIQEATRKIPVQYAKRVVGRKMYGGQSSHIPMKVNQSGVMPIIFASSLLAFPQ
                     TIAMFMGPNAQAFVQKYLSMATEQGFWTYRSIEILLIIFFSYFYTTVSFNTEDISKNM
                     KNNGGFIPGIRPGEPTMDYLNRILTRLTLAGATFLAIIAMVPALTTHYMKVNMSLAGT
                     SLLIVVGVALELKRQLESNLVMRSYQGFLK"
     gene            11117..11767
                     /locus_tag="ACGI59_002186"
     CDS             11117..11767
                     /locus_tag="ACGI59_002186"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFG9739972.1"
                     /translation="MRIILLGPPGAGKGTQAAGIVEKYNIPHISTGDIFRKNIKEGTE
                     LGKKAKEYMDQGLLVPDELTVGLVTDRISQEDCKNGFMLDGFPRNVAQGEHLDIFLKN
                     AGISLDKVVNIEVDKSILVSRAVGRRICKSCGATYHVEFNPPKVEGVCDVCQGELYQR
                     ADDNEETVSKRIQVYLDETKPLVDYYSKQGIIADIKGDQAIDKVFEDIVAALGSGK"
     gene            11768..12514
                     /gene="map"
                     /locus_tag="ACGI59_002187"
     CDS             11768..12514
                     /gene="map"
                     /locus_tag="ACGI59_002187"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFG9739973.1"
                     /translation="MIILKSKKEIELLREAGKIVADTHEVLRKAISPGISTLELDKIA
                     EENIRKYNAEPSFKGYGGFPGSICASINREVVHGIPGETILQEGDIVSLDIGAYYKGY
                     HGDSAKTHGVGMISEEDRKLIEVTRESFYEGIKFAKLGYRLSDISHAVQTHVEKHGFS
                     VVRDLVGHGVGANLHEDPQVPNYGLPGKGPRLREGMVIAIEPMVNAGRYHVKTLSDGW
                     TTVTIDGKKSAHYEHTIAITEHEPLILTKL"
     gene            12536..12805
                     /locus_tag="ACGI59_002188"
     CDS             12536..12805
                     /locus_tag="ACGI59_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KOW domain-containing protein"
                     /protein_id="HFG9739974.1"
                     /translation="MLSDNLSIGQVVKVSLGRDKGNLFFVVKIINNEYVLIADGKKRK
                     LDKPKLKKVKHLKKYNFINDEVRKRVVSGQEITDSFLRAELTKLN"
     gene            12843..13061
                     /gene="infA"
                     /locus_tag="ACGI59_002189"
     CDS             12843..13061
                     /gene="infA"
                     /locus_tag="ACGI59_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017185218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFG9739975.1"
                     /translation="MAKKDVIELEGTVSEALPNAMFKVKLENGHEILCHISGKLRMNF
                     IRILEGDKVNVELSPYDLTRGRITWRKK"
     gene            13097..13210
                     /gene="rpmJ"
                     /locus_tag="ACGI59_002190"
     CDS             13097..13210
                     /gene="rpmJ"
                     /locus_tag="ACGI59_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFG9739976.1"
                     /translation="MKVRPSVKPICEKCKVIKRKGKVMVICENPKHKQKQG"
     gene            13363..13734
                     /gene="rpsM"
                     /locus_tag="ACGI59_002191"
     CDS             13363..13734
                     /gene="rpsM"
                     /locus_tag="ACGI59_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFG9739977.1"
                     /translation="MARIAGVDLPREKRAEIGLTYIYGIGKATANEILAKAEINPDTR
                     IKDLSEDQVNELRKVIDDDFLVEGDLRREIALNIKRLRDIKCYRGIRHAKGLPLRGQR
                     TKTNARTRKGPRKTVSRKKKK"
     gene            13770..14168
                     /gene="rpsK"
                     /locus_tag="ACGI59_002192"
     CDS             13770..14168
                     /gene="rpsK"
                     /locus_tag="ACGI59_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020449874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFG9739978.1"
                     /translation="MAKPKKKVTRIRRRERKNIERGHAHIQSTFNNTIITLTDVHGNA
                     ISWASSGQLGFKGSRKSTPFASQMAAETAAKAAMEHGLKSVEVFVKGPGSGREAAIRA
                     LQATGLEVTMIKDVTPIPHNGCRPPKRRRV"
     gene            14200..14823
                     /gene="rpsD"
                     /locus_tag="ACGI59_002193"
     CDS             14200..14823
                     /gene="rpsD"
                     /locus_tag="ACGI59_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFG9739979.1"
                     /translation="MARYTGASCRQCRREGMKLFLKGDRCYTDKCAIVKRNYAPGQHG
                     QGRKKVSNYGLQLREKQKVKRIYGVLETQFRNLYERAENMPGKAGENLLSLLERRLDN
                     VVYRMGLASSRKEARQLVTHGHFTLNGNKVDIPSLIVKVGDVIEVKEKSRSSAKFKNL
                     VEVNSRIAPKWLEANVEGMTAKVVGVPTREDIDLEIAEHLIIELYSK"
     gene            14901..15848
                     /locus_tag="ACGI59_002194"
     CDS             14901..15848
                     /locus_tag="ACGI59_002194"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFG9739980.1"
                     /translation="MIEIEKPKVDIVELSEDYRYGKFVIEPLERGYGITIGNALRRIL
                     LSSLPGVAVNAIKIDGVLHEFSTIPGVKEDVTEIILTLKELSATIDGEGSRTLKIEAQ
                     GPCSITGADIICPPDVEILSKDLAIATLDDNAKLNMEIFVDKGRGYVSAEENKTENVP
                     IGVLPVDSIYTPVEKVSYHVENTRVGQKTDYDKLVLEVWTNGSINPQEGISLAAKVLV
                     EHLNLFIDLTEHVSSVEIMVEKEEDQKEKVLEMTIEELDLSVRSYNCLKRAGINTVEE
                     LANKSEDDMMKVRNLGKKSLEEVIQKLEELGLGLKPSEE"
     gene            15869..16210
                     /gene="rplQ"
                     /locus_tag="ACGI59_002195"
     CDS             15869..16210
                     /gene="rplQ"
                     /locus_tag="ACGI59_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFG9739981.1"
                     /translation="MAKYRKLGRETAHRNLMLRNLVTCLLRSGRIETTVTRAKETRRM
                     AEKMITLAKRGDLHARRQVLAYVMDETVVNNLFTDLAPKYAERNGGYTRIIKIGPRKG
                     DAAEMAFIELV"
     gene            16330..17163
                     /locus_tag="ACGI59_002196"
     CDS             16330..17163
                     /locus_tag="ACGI59_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HFG9739982.1"
                     /translation="MDNIVKVNNISFEYITDEAKLKAIDNLSLDVKKGEFVAIIGHNG
                     SGKSTLSKNLNAILMPTEGNILIDDMDTKEEERLWDIRQTAGMVFQNPDNQIVATIVE
                     EDVAFGPENLGIEPKEIRRIVEESLKSVGMYDLRDRQPHLLSGGQKQRVAIAGIIAMR
                     PKCIIFDEATAMLDPSGRKEVMKTIKRLNKEENITVIHITHFMEEAVEADRVVVMEKG
                     KKILEGTPREVFSKIKMLKEIGLDVPCMTELSSLLIEEGINISSDILTVDEMVMELCQ
                     L"
     gene            17151..18017
                     /locus_tag="ACGI59_002197"
     CDS             17151..18017
                     /locus_tag="ACGI59_002197"
                     /EC_number="3.6.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HFG9739983.1"
                     /translation="MSIIVKNLTHIYNEGMPFASKALDDVSFEIKDRDFVGLIGHTGS
                     GKSTLIQHLNGLLKPSSGEIFINDFNITDKNLNLTEIRKRVGVVFQYPEYQLFEETID
                     KDIAFGPSNLGLEESEIHNRVKASMEAVGLDYEGFKDKSPFELSGGQKRRVAIAGVIA
                     MNPEVLILDEPTAGLDPGGRDEIFNLIKDLHEKKNMTIILSSHSMDDMAKLAKTLIVM
                     NHGSVEFMGTPREVFKSNASKLKDIGLDIPQVLELALKLREKGFDISEDILTLEEAKQ
                     EILKVVRGRGLC"
     gene            18011..18814
                     /locus_tag="ACGI59_002198"
     CDS             18011..18814
                     /locus_tag="ACGI59_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFG9739984.1"
                     /translation="MLKDITIGQYYPTSSAIHKLDPRIKLVATIVFMVSIFVVNKFWP
                     YIVVLLCLLAMIKLANIPVKYIVKGVKPLKWIILFTFLINIFFLPGDEIWSFGFLAIT
                     KQGLRQAIFMAIRLIFLVVGTSLLTLTTSPIELTDGIERLLNPFKKIGLPVHELAMMM
                     TIALRFIPTLLDETDKIMKAQMSRGADFESKNLINRAKNLVPLLVPLFVSAFRRADEL
                     AMAMEARCYRGGHNRTKMRESVISRRDYMACVFQVVYLGAIIATRFIAI"
     gene            18846..19577
                     /gene="truA"
                     /locus_tag="ACGI59_002199"
     CDS             18846..19577
                     /gene="truA"
                     /locus_tag="ACGI59_002199"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFG9739985.1"
                     /translation="MRNIKIKIQYNGKNYCGWQKQPDSLGIQGTIERAIYDITKEETS
                     LIGSGRTDSGVHAIGQIANFKINSGISIESIPMALNAKLPKDISVIEACEVNDDFHSR
                     YSAKGKTYKYLVYNSKFRNPILSEISYQVKYELDFDKMCSEAKSLLGTHDFKGFMSSG
                     SSVKDTVRTIYDIDISKKDDLITFEISGNGFLYNMVRIIVGTLVDMGRGRINEPFLDI
                     IQSKTRSRCGHTAPAQGLFLKKVHY"
     gene            19697..20128
                     /gene="rplM"
                     /locus_tag="ACGI59_002200"
     CDS             19697..20128
                     /gene="rplM"
                     /locus_tag="ACGI59_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFG9739986.1"
                     /translation="MKSYIAKPADVQRKWYLVDAEGKTLGRLATEIATVLRGKHKPTF
                     TPHVDGGDFVVVVNAEKIVLSGKKLDQKYYRYHTGYVGGLKEISYRDMMDKKPEEVIS
                     HAVSGMLPKNKLRSRMMTRLRVFAGAEHTHAAQNPEVLNFK"
     gene            20157..20549
                     /gene="rpsI"
                     /locus_tag="ACGI59_002201"
     CDS             20157..20549
                     /gene="rpsI"
                     /locus_tag="ACGI59_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFG9739987.1"
                     /translation="MANVQYYGTGRRKSSVARVRLVAGEGNILVNGRALENYFNYETL
                     IRDVKQPLVLTGNENKYDVIVKVEGGGFTGQAGAIRHGISRALLKADLDLRPALKKEG
                     FLTRDARMKERKKYGLKAARRAPQFSKR"
     gene            21056..21760
                     /gene="cwlD"
                     /locus_tag="ACGI59_002202"
     CDS             21056..21760
                     /gene="cwlD"
                     /locus_tag="ACGI59_002202"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase CwlD"
                     /protein_id="HFG9739988.1"
                     /translation="MRKYIKHIIFSFAMICLVVVSIFEIKNISEDVIKYMPVTNKTII
                     LDAGHGGIDPGALNKDKSTSEKDINLAITLKLRELIESSGGLVILTREDDSSLYKEEN
                     NKTTRQKYNENLKNRKEIISNSNANMFVSIHLNAFEQSKYYGAQTFYPKDKQDSKELS
                     KCIQEELKRVVDKTNNREVKPRDDIYLLKENNIPSVLIECGFLSNEKECKLLTDETYQ
                     EKIAWAIYIGIQKYLS"
BASE COUNT         8816 a         2563 c         4493 g         6070 t
ORIGIN      
        1 ctttactaaa ctaatggatt taatataaaa aaaagagggg gtaactcctc tcttttttgt
       61 tttttgttga caagtagcgc taaagcatat atactaaact agtgccttga gacagattaa
      121 caatcaagtt taaggttatg atgttgtaat agaaaataaa attgaaaaaa acagaaaaaa
      181 aacttgaaaa aatgtaacag atgatataaa atataatagt gtgcttgaga gattaagaaa
      241 ataataatta aatcctagtg aaatacgaac tgatatcgat ttaaaagtta caacacaccc
      301 ggagcagtgt atcggtattg gttgtacgca acttgaaaat taaacacgtg ataaaggagg
      361 gaaaacaaaa tggctaagaa tgaaaaaata agaataagat taaaatcata tgatcacaaa
      421 ttattagatt tttcagcagg taaaatagtt gaaactgcta aaaaggctgg atcacaagtt
      481 tcaggacctg tgcctctacc aacagaaaaa caagttgtaa ctatattaag agctgtacat
      541 aagtacaaag actctagaga acaatttgaa ataagaactc ataaaagact aatcgacata
      601 gctaatccaa cacctaagac agttgactca ttaatgagat tagacttacc agcaggtgta
      661 gatatagaaa taaaattata agaaccccta agatattatc ctatagatga ttatcttaga
      721 atgattactc taaaagatgt aatccgctgt aagaattata ggaggtgcta atatgaaagg
      781 aatattaggc aaaaaagttg gtatgactca aatattcact gataaaggtg tagttatacc
      841 tgtaacagct gttgaagcag ggcctatggt cgttactcaa ataaaaacag ttgataaaga
      901 tggatacaat gctatacaaa taggttttga agatgctaaa gaaaaagctt taaacaaacc
      961 taaaaaagga catttagctg ctgctaatgt tttaaagaaa catttaaaag aatttagagt
     1021 agattctgta gaaggataca cagtaggaca agaaataaaa gctgatgtat ttgaagcagg
     1081 tgcaaaaata gatgttactg gaataagtaa aggtaaggga ttccaaggtc caataaaaag
     1141 acatggacaa agtagaggtc ctgaaactca cggttctaga taccacagaa gaccaggttc
     1201 aatgggagct tgttcttacc caggtagagt atttaaaaac aaaaaattag ctggacatat
     1261 gggaagcgta aaggtaacag ttcaaaactt agaggttgtt aaagtagatg ctgacaagaa
     1321 ccttatatta gttaaaggag ctataccagg agctaaaggt tcagtagtaa ctataaaaga
     1381 agctataaag gtttctaaat aatgactaac ctagagaaag gaggaataac aatgccaaaa
     1441 ttaaatgtac taaatgttag tggacaaaac gttggagaaa tagaattatc agattctata
     1501 tttggcgtag aagttaatgg acatgtttta tacgaagttg taaaaaatca attagcaaat
     1561 aagagacaag gaactcaatc tgctaaaact agagcagaag ttagaggtgg cggaagaaaa
     1621 ccttggaaac aaaaaggaac aggtagagca agacaaggtt ctacaagatc tgtacaatgg
     1681 gtaggtggag gagttgcttt tgcacctaaa ccaagaagct acaaatatac attacctaag
     1741 aaggtaagaa gattagctat gaagagtgct ttatcttcaa aagttcaaaa tagtgaagtt
     1801 atagtattag atgcattaaa catggatgct cctaagacta aagaatttgc tcaaatatta
     1861 aacaatataa atgctgctaa aaaagcttta gtagttatag ctgacaaaaa cgataacgta
     1921 ataaaatcag ctagaaatat agaaggtgtt caaactgcat tagttaatac tatgaatgta
     1981 tatgatatat taaaatacga ttcatttata ataactacag atgctgttaa aaaagtggag
     2041 gaggtgtacg cataatgact aatccacatg atgtaataat aagaccagtt gtaactgagc
     2101 acagtatggc tgaaatgggt gaaaaaaaat acacttttgt tgtggctaaa gatgcgaaca
     2161 aaactgaaat aaaaaaagca gtagaaaaag tattcggagt tagtgttgat aaagtaaata
     2221 ctttaaacta cgatggaaaa gttaaaagaa tgggtagaac tcaaggaaga acatcaagct
     2281 ttaagaaagc agtagttaaa ttaactgctg atagtaaaga aatagaattc ttccaaggaa
     2341 tgtagtatta taacaaacga aggagggaaa acagatggct ataaaaaagt ttagaccaac
     2401 ttctcctgcc ttaagacaaa tgacagtttt agtttctgat gaaataacat gtaatcaacc
     2461 agaaaaatct cttttagtta acttaaagaa aaatgctggt agaaacgtac atggtagaat
     2521 aactgttcgt catagaggtg gaggacaaaa aagaaaatat agaataatag attttaaaag
     2581 agataaagat ggtatacctg ctaaggttgc tactatagaa tatgatccaa acagaactgc
     2641 aaatatcgca ttattaaact atgctgatgg agaaaaaaga tatatattag ctccagttgg
     2701 aattaacgtt ggagatacta tattatcagg attaggtgct gatataaaac caggaaactg
     2761 tttagcatta aaagatatgc cagttggtac tatcattcat aatatagaat taaagccagg
     2821 aaaaggagct caattagtta gatctgctgg agtttctgca caattaatgg ctaaagaagg
     2881 aaaaaatgct ttattaagat taccatcagg tgagatgaga ttagtaagca taaactgtaa
     2941 agctactata ggtcaagttg gaaatataga gcatggtaac gtagttatcg gtaaagcagg
     3001 tagaaaaaga catatgggaa taagacctac tgtaagaggt tctgtaatga accctaatga
     3061 ccatccacac ggtggagggg aaggtagatc tccaataggt agaccatctc ctgttactcc
     3121 atggggtaaa ccagctcttg gatacaaaac tagaaagaaa aataaagctt caaataaact
     3181 aatagtatca agaagaacta agtagtaaat ttatttttgc tcggaaagga ggaatataaa
     3241 tgtcaagatc aactaaaaaa gggccttttg tccatgcaag acttttaaag aagatagaag
     3301 ctatgaatgc tagtggaaat aaagaagtta taaaaacttg gtcaagatct tcaactgtat
     3361 ttccacaaat ggtagaaaac actatagctg ttcatgatgg aagaaaacat gttccagtat
     3421 atataacaga agatatggtt ggacataaat taggtgagtt cgttcctact agaactttca
     3481 aaggacataa ggatgacgaa aaatctaata agagaaaata attaaattat gactaagaaa
     3541 ggaggaataa caatggaagc aaaagctact gctaaatacg tacgtgtatc accaagaaaa
     3601 gcaggccaaa tatgtgacct tgttagagga aagaatgttg atgaagcatt agcaatatta
     3661 aagtttactc caagaggagc agcttcaata atagctaagg ttgtaaaatc agctaaagct
     3721 aacgcagaaa acaatcacga aatggatact gaaaaattat atatagcatc aatagttgct
     3781 aatcaaggac caacaatgaa gagattcatg cctagagcta tgggtcgtgc aactacaata
     3841 agaaaaagaa cttctcatat agaggttgtt gttaaagaaa aaaaataatt aatagatagg
     3901 agggaaagta atgggtcaaa aggttaatcc acacggctta agggtcggtg ttataaaaga
     3961 ttgggactca agatggttcg ctactgataa aaaagagttc ggaaacttat tattagaaga
     4021 ccataatata cgtaaattct taaagaaaag attatactca gctggagttg ctaagataga
     4081 aatagaaaga tcagctaaca aaataaaaat ggacttacac gttgctaagc ctggtgtagt
     4141 tataggaaga gctggtgcag gaatcgaagc attaaaagct gaattagaaa aaatgacaaa
     4201 gaaaactata atagttaata tagtagaagt aagaagtaca gataaaaatg ctcaattagt
     4261 agctgagaat atagctttag ctatagagag aagggttgct ttcagaagag caatgaaaca
     4321 agctatacaa agagcaatga aatctggtgc taaagggata aaagtttctg cttcaggaag
     4381 attaggtgga gctgaaatgg ctagaactga aggttatagt gaaggaaatg tgccactaca
     4441 aactttaaga gcagatatag attatggttt cgctgaagct gatacaactt atggaaaaat
     4501 cggtataaaa gtttggatat gtaatggtga agttttacca actagagacg gtgtaaatcc
     4561 aagagaagaa agcagaaaga gcgatagaag agacaacaag agagataata gaagaaacga
     4621 tagaagagga aatgacagaa gaggaaatga taacagagga aactacagag gacaaagacc
     4681 tcaaggtgga tcaagacctc aaagaactga aaacaaagga aattaataat agttaggttc
     4741 aaggaaggag gaaaaatact catgttaatg ccaaaaagag taaagcgtcg tagagttcat
     4801 agaggaagca tggctgggca agctcataag ggtaataaag ttacttatgg tgagtttgga
     4861 ttagtagcat tagaagcttc ttggataact tctaatcaaa tagaagctgc cagaatcgcg
     4921 atgactagat atataaaaag agggggaaag gtttggataa aaatattccc tcataaacca
     4981 gtaacaagaa aaccagcaga gactcgtatg ggtgcaggga aaggttctcc agaatattgg
     5041 gtagctgtag ttaagccagg aagagttatg tttgaattag caggtgtttc tgaagataaa
     5101 gcaagagaag ctatgagact tgctgcacat aaattaccaa tcaaatgtaa atttgttaaa
     5161 aaagaagatt tagaagtaaa gggtggtgaa taggatgaaa gctaaagaat taagagattt
     5221 aacaagcgaa gagctaatga acaaattaaa cgacttcaaa agtgaattat ttagcttaag
     5281 attccaatta gctactggtc aattagaaaa tacagctaga ataaagtttg ttaagaagga
     5341 tatagcaaaa gttaaaactg ttcttgctga aagaaagtta tacgaaacta gagcttaatt
     5401 tggaaaggag gctttttaaa catggaaaga ggaagaagaa aagttagaat aggccgtgtt
     5461 gttagtgata agatggataa aacaatagta gtagctgttg aagaattcgt acgtcatcca
     5521 ctatataata aacgtgttaa gagaactaag aaattcaagg ctcatgatga aaagaatata
     5581 tgtaacatcg gagatagagt taaaataatg gaaactagac cattatctaa agataagaga
     5641 ttcagactag ttgaagttgt tgagaaagtt aagtagtttt tgaagaagga gggattacga
     5701 tatgatacaa caagaatcac gtctaagagt tgctgataac tcaggagcta aagaactttt
     5761 atgtatacgt gttctaggcg gaagtaaaag aagatatggt aacataggcg acgttatagt
     5821 tgctactgtt aaaagtgcaa cacctggtgg agttgtaaaa aaaggtaagg ttgttaaagc
     5881 tgttatagta agaagcaaac aaggcgtaag acgtaatgac ggaagctata tatcttttga
     5941 tgaaaatgct gctgttataa taaaggacga taaaactcca gtaggaactc gtatattcgg
     6001 gcctgttgct agagagttaa gagacaatga attcatgaaa atagtttctc ttgctccaga
     6061 agtactataa ttaaggaggt gcaataggac atgatgcgtg ttaaaaaagg tgacactgtt
     6121 gtagttatag caggtaaaga taaaggtaaa aaaggttcag ttttaaaggt atatcctaaa
     6181 actagcaaag tgttagttga aggtgtaaat gtaataacta aacatcaaaa accaagtgct
     6241 atgaaccaac aaggtggaat aataaataaa gaagccccaa tacacatatc taatgtaatg
     6301 ccatttgatc ctgaaacagg aaaaggcgtt agagtaagat atgaagtaaa agatgggaac
     6361 aaagtaagag tatcagcaaa gagcggaaaa gaattataat aaatattgct tgaaaggagg
     6421 gaccttaaat ggcttctaga ttacaagaaa aatacatgaa agaagttgct cctgctttaa
     6481 tggagaaatt tggatacaaa aacgtaatgg agatacctaa gttaaataaa atagttatta
     6541 acatgggtat aggtgacgct agagaaaatc caaaaggatt agaaaaaggc gttgaagaat
     6601 tagaaatgat atcaggacaa aagcctgtta taactaaagc tagaaaatct gttgctaact
     6661 tcaaattaag agaaggaatg ccaataggaa caaaagttac attaagagct gacaaaatgt
     6721 tctactttat ggacaaatta gtttcagttt ctttaccaag agttagagac tttagaggag
     6781 ttaaccctaa tgcttttgat ggtagaggaa attatgcttt aggagttaaa gaacaattaa
     6841 tattccctga aatagaatat gataaaatag ataaagtaag aggaatggat ataatatttg
     6901 ttactacagc aaaaactgac gaagaagctc gtgaattatt aaaattatta ggaatgccat
     6961 tttctaagta agcaaaggag ggattcccgt ggctagaaaa gcgatggttg ttaagcaaca
     7021 aagaaagcaa aagtacgcta ctagagaata cactagatgt actatatgcg gaagaccgca
     7081 ttcagttttg aaaaaatttg gtatatgccg tatatgcttt agagaattag cttataaagg
     7141 tcaaatacct ggtgttagaa aagcaagctg gtagaagctt gattagaatg gaaggagggc
     7201 aacaaaatga caatgacaga tccaattgca gatatgctta ctcgtataag aaatgctaat
     7261 gttgttaagc atgaaactgt agatgttcca gcttctaata tgaagaaaga attagctaga
     7321 atcttattag aagaaggttt cataagaggt tatgatgtta tagaagatgg aaaacaaggt
     7381 atcataagaa tacaattaaa gtacggacaa gaaggcgaaa gagttataac aggtcttaag
     7441 aaaatatcta agcctggaat gagagtttac gctgctaacc atgagatacc aaaagtatta
     7501 aacggattag gaatatcagt tatatctact tcaaaaggaa tattaactga caagcaagct
     7561 agaaaagaaa atgttggtgg agaagtaatc tgctacgttt ggtaattaat atgaacaatg
     7621 taaggaggtg cgaccatgtc aagaataggt gttaagccaa taataatacc agcaggtgtt
     7681 gaagttacta tagctgaagg aaatttagtt acagtaaaag gtccaaaagg aactttaact
     7741 aaacaattaa gtgctgagtt aaacataaaa aaagaagaaa atacaataat ggttgaaaga
     7801 ccaactgata ataaaaaaca tagatcttta cacggactaa ctagaacttt attagataat
     7861 atggttgtag gtgtaaatac tggtttcgaa aagaaattag aattaaaagg tgttggatat
     7921 agagctcaaa aacaaggaaa gaaattagtt atgaacttag gtttctctca tccagttgag
     7981 atggaagatc cagaaggaat aactgttgaa gctccaaacc aaacagagtt aatagtaaaa
     8041 ggtatagaca agcaattagt agggaattat gctgctaaga taagagcttg gagaaagcca
     8101 gagccataca aaggtaaagg tataaaatac gttgatgaag taatcagacg taaagaagga
     8161 aaaactggta aaaaataata ccaaaactta aacgctagaa aggagtgagc tagatgttaa
     8221 aaaaggctga taaaaatgct aatagacttc aaagacataa gagagttcgt agaaaaataa
     8281 gcggtacttc tcaaagacca agattatgtg tatttagaag tgctaacaat atatatgctc
     8341 aaataataga tgacacaaaa agagtaactc tagttgctgc atcttcttta gaagcagaag
     8401 ttaagagtgc tgtcaatcat acaggaaaca aggaagcagc taagaaagtt ggagaattag
     8461 ttgctaaaaa agctgttgaa aaaggaatca ctgaagttgt atttgacaga ggtggatact
     8521 tatatcatgg aagaattcaa gaattagctg aaggtgctag agaagctggt cttaagttct
     8581 aatacgaagg agggaaaaat acatgctacg tcgtaagccg atagatgcag gacaacttga
     8641 cttacaagaa aaagttgttg aagtaagacg tgttactaag gttgtaaaag gtggtagaaa
     8701 tttcagattt gcagcattag tagttgttgg agatgaaaac ggacacgttg gtataggtgc
     8761 tgggaaagca atggaagtac cagacgctat aaaaaaagca gttgaagatg ctaagaaaaa
     8821 tctaatagta gtgccaatag ttgggactac aattcctcac gaagtacgcg gacactttgg
     8881 tgctggaaat atattaataa tgcctgctgt agaaggtaca ggagtaatag ctgggggacc
     8941 tgctagagcc gtacttgagc tagctggact aaaagacgtt agagctaaat ctttagggtc
     9001 taacaatcca agaaatatgg taaatgctac aatagaagga cttaattctc taaaaacagt
     9061 tgaagatata gctaaactta gaggtaaaaa agttgaagaa cttctaggtt aaggaggtag
     9121 tgtaaaatgg ctaaattaca aataaagtta gttagaagtg ttataggaac tactcctaac
     9181 caaaaaaaga atgttgaagc gttaggatta agaaaaagag aacaagttgt tgtaaaagaa
     9241 gacaatgctc aaacaagagg tatgataaat aaagtaagtc acttattaga agtaactgaa
     9301 atagctgaat aatttttata aagtagtgca aggaggtgca atccatgaag ttacatgagt
     9361 taaaacctgc tgaaggtgca gtaagagcta agagaagatt aggtagaggt actgcaactg
     9421 gacaaggtaa aactgcagga cgtggacaaa aaggtcaatg gtctcgttct ggtggtggag
     9481 taagagtagg atttgaaggt ggacaaatgc cactagctag aagacttcct aagagaggtt
     9541 tcaataacat atttaagaaa gtttacacag aagttaatgt tgaagtttta aacagatttg
     9601 aaaatggaac agaaataact gcagaattat taaaatctac taaaactata agcaaaatag
     9661 gtaaagacgg aattaaaatc ttaggtgaag gtaatttaga gaaagcttta actgttaaag
     9721 ctgctaaatt tacagcttca gctcaagaaa aaatagaaaa agctggtgga aaagcagagt
     9781 tagtataata acctgctaat ctcctagcca gtacaggcgg ggtgaattag cgtgctgtca
     9841 aaattaaaac aagcttggaa gattaaagca gtaagaaaaa aagtaatgta tacacttatg
     9901 atgatcgtga tattcagaat aggtactact ataccagttc caggcataga cactagtatt
     9961 atacaaaaga tggtaggcgg caatagtcta ctttctctgt ataatatgtt tactggtgga
    10021 gcatttagta acttttcact atttgcttta ggtataagtc catatatcac tgcatcaatc
    10081 ataatacaac ttctaacagt aggttttgaa agtttagcag aacttcaaaa atctggtgaa
    10141 gaaggaaaga aaaaaatcaa taagtacact aaatatacag cactagctct agcagttgta
    10201 caagctttgg gaattacatt aggaattgta agaagtgctt taatatcaaa tagtgtgttt
    10261 tttatcacaa ctgttgtaat aacattagtt tcagcaagta tgttagtaat gtggattggg
    10321 gacaaaatta ctgaaaaagg aatcggtaat ggtagttcag taataatatt tgcaggtata
    10381 atatcaagaa tacctacaga tgttattaaa atatcgcaac aagttaaatc tggcgaagtt
    10441 gcaccttggg ttatagtgat tctagctgta gttatattac ttacagtaac aggagttaca
    10501 tttatacaag aagcaactag aaagatacct gtacaatatg ctaaaagagt tgttggaaga
    10561 aaaatgtatg gaggacaaag ttctcatata ccaatgaaag ttaaccagtc aggagttatg
    10621 cctataattt ttgctagttc acttttagct ttccctcaaa caatagctat gtttatgggt
    10681 ccaaatgctc aggcttttgt tcaaaaatac ttaagtatgg caacagaaca aggattttgg
    10741 acatatagat caattgagat tctacttata attttcttct catattttta tacaactgta
    10801 tcatttaata cagaagacat atctaaaaat atgaaaaata atggtggatt tataccaggt
    10861 ataagaccag gtgagcctac tatggattat ttaaatagaa ttttaactag attaacttta
    10921 gctggagcta ctttcttagc aataattgct atggtgccag ctcttacaac acattatatg
    10981 aaagtaaata tgagtctagc tgggacttca ttgctaatcg ttgttggagt tgctttggag
    11041 cttaagagac agttagagtc aaacttagtt atgagaagct atcaaggttt cttaaaataa
    11101 aatggagatg gataatatga gaataatatt acttggacct ccaggtgctg gtaaaggtac
    11161 tcaggcggca ggaatagtag aaaaatacaa tatacctcat atatcaacag gagatatatt
    11221 cagaaagaat ataaaagagg gaacagaact tggaaaaaaa gctaaagaat acatggacca
    11281 aggtttatta gtaccagatg agttaactgt aggtttagtt actgatagaa tatctcaaga
    11341 agattgtaaa aatggattta tgttagatgg atttccaaga aatgtagcac aaggagaaca
    11401 tttagatatc ttcttaaaaa atgctggtat atcactagat aaagttgtca atattgaagt
    11461 tgataagagt atattagtgt ctagagcagt tggtagaaga atatgtaagt cttgtggagc
    11521 tacttaccat gttgagttta atcctcctaa agtagaaggt gtatgtgatg tatgccaagg
    11581 agaattatat caaagagctg atgataatga agaaactgta tctaagagaa tacaagttta
    11641 tctagatgaa actaagcctt tagtagatta ttatagcaaa caaggtataa tagcagatat
    11701 aaaaggtgat caagcaatag ataaagtatt tgaagatatt gtcgcagcct taggaagtgg
    11761 aaaataaatg attatcttga aatcaaaaaa agaaatagaa cttttaagag aagcaggtaa
    11821 aatagttgct gacactcatg aagttttaag aaaagctatt tctccgggaa tatctacttt
    11881 agaattagat aaaatagctg aagagaatat aagaaaatac aatgcagaac cttcttttaa
    11941 aggatatgga ggttttccag gaagtatatg tgcttctatc aatagagagg tagtacatgg
    12001 tatcccagga gaaactatat tacaagaagg tgacattgta agtttggata taggagccta
    12061 ttataaagga tatcatggtg attctgcgaa aactcacgga gtaggcatga tatctgaaga
    12121 agataggaaa ttgatagaag taactagaga aagcttctat gaaggaataa aatttgctaa
    12181 attaggatat agactttcag atatctcaca cgcagtacaa acacacgttg agaaacatgg
    12241 tttctcggta gttagagatt tagtgggtca tggagttgga gcaaatcttc atgaagaccc
    12301 acaagtacct aattatggac tcccaggaaa aggaccaaga cttagagaag gaatggttat
    12361 tgctatagag ccaatggtta atgcaggtcg ttatcatgta aagactctat cagatgggtg
    12421 gacgactgtt acaatagatg gaaaaaaatc agctcattat gagcatacga tagcaattac
    12481 tgaacatgaa cctttgatat tgacaaagtt gtaatttaaa aagtaggtga aaaaagtgct
    12541 atcagataat ttaagtatag gtcaagttgt taaagtttca ttaggacgag ataaaggaaa
    12601 tttattcttt gtagtcaaaa taattaataa tgaatatgta ttaatagctg atggaaaaaa
    12661 gagaaaactt gacaaaccta agttaaagaa agtgaagcac ttaaaaaaat ataattttat
    12721 taatgatgaa gttagaaaaa gagtagtatc tggacaagag attacagatt cttttttaag
    12781 agctgaactt actaagttaa attagataat ttaacttatt gaaatggagg gctggttagt
    12841 taatggccaa aaaagatgtt atagaattag aaggtacagt ttcagaagct ttacctaatg
    12901 ctatgttcaa agttaaacta gaaaatggac atgagatatt atgccacatt tctggaaagc
    12961 taagaatgaa cttcataaga attcttgaag gtgacaaggt gaatgttgaa ctttctccat
    13021 atgaccttac aagaggaaga attacttggc gtaagaagta gactatatta atatttagtc
    13081 taaggaggga ttaataatga aggttagacc atcagtaaaa ccaatatgtg aaaaatgtaa
    13141 agtaataaaa agaaaaggaa aagtaatggt tatctgtgaa aatccaaagc ataagcaaaa
    13201 gcaaggataa gaaattatta ctagcatttt gttaaatttt atagtataat actatattgt
    13261 gatgttaaca aaagaacgtt aaaatcgttg aacaatctgg cgtaggattg gcatatgatg
    13321 tgaagtttta aacactaaaa actatgtgta ggaggtgcga tcatggcaag aatagctggg
    13381 gtagatttac ctagagaaaa aagagcggag ataggcttaa cttatatata tggtataggt
    13441 aaagcaactg ctaacgaaat attagctaag gctgagataa atccagatac tagaataaaa
    13501 gatttatcag aagatcaagt taatgagtta agaaaagtta tagatgacga tttcttagtt
    13561 gaaggtgact taagaagaga aattgcttta aacataaaga gattaagaga tataaaatgt
    13621 tatagaggaa taagacacgc taaaggtctt ccattaagag gacaaagaac taagactaat
    13681 gctagaacta gaaaaggtcc tagaaaaaca gtatctcgta aaaagaaaaa ataaataaat
    13741 aataactaga tgaaggaggg aaaaggaaaa tggctaaacc aaaaaagaaa gttacacgta
    13801 ttagaagaag agaacgtaaa aatatagaac gtggtcatgc tcatatacaa tcaactttca
    13861 ataatacaat aataacttta actgacgttc acggaaatgc tatatcttgg gcaagttctg
    13921 gacaattagg attcaaagga tcaagaaaat caactccatt tgcatctcaa atggctgctg
    13981 aaacagctgc aaaagctgcg atggaacacg gactaaaaag tgttgaggta ttcgtaaagg
    14041 ggccaggttc aggaagagaa gctgcaataa gagctttaca agcaactgga ctagaagtaa
    14101 ctatgataaa agacgttact ccaatcccac ataacggatg cagaccacca aagagaagaa
    14161 gagtgtaatt aataagagta caataggagg tgtagataaa tggcaagata tacaggtgca
    14221 tcatgtagac aatgccgtag agagggaatg aaattattcc ttaaaggtga tagatgttat
    14281 acagacaaat gtgctatagt aaaaagaaac tatgctccag gtcaacatgg ccaaggaaga
    14341 aagaaagttt ctaactatgg attacaatta agagaaaaac aaaaagttaa aagaatatat
    14401 ggagttttag agactcaatt tagaaattta tatgaacgtg ctgaaaatat gcctggtaaa
    14461 gcaggtgaga acttattaag tttattagag agaagattag ataatgtagt ttacagaatg
    14521 ggactagcat cttctagaaa agaagctaga caattagtga ctcatggtca tttcacttta
    14581 aatggaaata aagtagacat accttcatta atagttaagg ttggagatgt tatagaagtt
    14641 aaagagaaat caagatcttc tgcaaaattc aaaaatttag tagaagttaa ctcaagaata
    14701 gctcctaagt ggttagaagc taatgtagaa ggaatgacag caaaagtagt tggtgttcca
    14761 acaagagaag atatagatct tgagatagca gagcacttaa tcatagaact ttactctaaa
    14821 taataagcac acttttaaaa attattttaa aattttgttt accctcagtg gattactgaa
    14881 ttttaaggag ggttttgtcc atgatagaaa tagaaaagcc aaaagtagat atagttgagc
    14941 ttagcgaaga ctatagatat ggtaagtttg ttatagagcc tctagaaaga ggatatggaa
    15001 taactatagg taacgcatta agaagaatat tattatcatc attaccaggt gtagcagtga
    15061 atgctataaa aatagatgga gttcttcatg aattttcaac aatacctggt gttaaagaag
    15121 atgttactga gataatatta actttaaaag agctttcagc aactatagat ggtgaaggaa
    15181 gtagaacact taaaatagaa gctcaaggac catgctctat cacaggtgca gatataatct
    15241 gtcctccaga tgttgaaata ttaagtaaag atttagcaat agctacatta gatgataatg
    15301 ctaaacttaa tatggagata tttgtagata aaggtagagg ttatgtttct gctgaagaaa
    15361 ataaaacaga gaatgttcca ataggtgttt tacctgtgga ttcaatatat actcctgttg
    15421 agaaggttag ttatcatgtg gaaaacacaa gagttggtca aaaaacagat tatgataaat
    15481 tagtattaga agtttggact aatggtagta taaatcctca agagggaata tcattagctg
    15541 caaaggtatt agttgagcat ttaaacttat ttatagactt aactgaacat gtaagtagtg
    15601 ttgaaatcat ggtagaaaaa gaagaagatc aaaaagaaaa agttcttgaa atgactatag
    15661 aagaattaga tttatcagtt agatcatata actgtttaaa gagagcggga attaatacag
    15721 ttgaagaatt agctaataaa tctgaggatg atatgatgaa ggttagaaac ctaggtaaga
    15781 agtcattaga agaggtaata caaaaactag aagaacttgg attgggatta aaaccaagtg
    15841 aagaatagcg agcaaaggag ggataagaat ggctaagtac cgtaaattag gacgtgaaac
    15901 agctcacaga aaccttatgt taagaaactt agtaacttgc ttactaagaa gtggaagaat
    15961 agaaactaca gtgactagag cgaaagaaac tcgtagaatg gctgaaaaga tgataactct
    16021 tgctaagaga ggagatcttc atgctagaag acaagtttta gcttatgtta tggatgaaac
    16081 agtagttaat aacttattca cagatttagc tccaaaatat gctgagagaa atggtggata
    16141 cactagaata ataaaaatag ggccaagaaa aggcgatgct gctgaaatgg cttttataga
    16201 attagtatag taaggaaagg attaggcttg atggcttaat ctttttttta tttttttgac
    16261 taaggtatga tataataatg tgtaaaatga tttttataag ttattgctta ctaaaaggag
    16321 tatgagttaa tggataatat agtaaaggta aataatattt cgtttgagta tattacagat
    16381 gaagcaaaac ttaaagcaat agataattta agcctagatg ttaaaaaagg agaatttgtt
    16441 gcgataatag gacataatgg ttctggtaag tctactttat ctaaaaactt aaatgctatt
    16501 cttatgccta ctgaaggaaa tattcttatt gatgatatgg atactaaaga agaagaaaga
    16561 ttatgggata taagacaaac tgcaggcatg gtatttcaaa atccagataa tcaaatagtt
    16621 gctactatag tagaagaaga cgttgcattt ggacctgaga acttaggtat agaaccaaaa
    16681 gaaataagac gaattgtaga ggagtcttta aaaagtgtag gtatgtatga tttaagagat
    16741 agacagcctc atttgttatc aggaggtcaa aagcaaagag ttgcaatagc aggaattatt
    16801 gctatgaggc ctaaatgtat aatatttgat gaagcaacag caatgttaga tccatctgga
    16861 agaaaagaag ttatgaaaac aataaaaaga cttaataagg aagaaaatat aactgttata
    16921 catataacgc actttatgga agaagcagta gaagctgata gagttgtagt tatggaaaaa
    16981 ggaaagaaaa tcttagaggg aactcctaga gaagtattca gtaaaataaa gatgttaaag
    17041 gaaatcggtt tagatgtacc ttgtatgaca gagttatcta gtttattaat agaagaaggt
    17101 ataaatatta gtagtgatat attaactgtg gatgagatgg tgatggaatt atgtcaatta
    17161 tagtaaagaa tttaacacat atatataatg aagggatgcc ttttgcaagt aaggcacttg
    17221 atgatgtttc ttttgagatt aaagatagag attttgttgg tcttataggt catacaggct
    17281 caggaaaatc tacattaata caacatttaa atggattact aaagccttcc tcaggagaaa
    17341 tatttataaa tgattttaat ataacagata aaaacttgaa tttaactgag attagaaaaa
    17401 gagtaggggt tgtatttcaa tatccagaat accaattgtt tgaagaaacg attgataaag
    17461 atatagcttt tggaccctct aatttaggct tagaggagtc tgagatacat aacagggtaa
    17521 aagcatcaat ggaagctgta ggacttgatt atgagggatt taaagacaaa tctccttttg
    17581 aattatctgg gggacaaaag cgtagagtag caattgctgg agttatagcc atgaacccag
    17641 aagttctcat attggatgaa ccaactgctg gtcttgaccc tggtggaaga gatgaaattt
    17701 ttaacttaat aaaagactta catgaaaaaa agaatatgac tataatacta tcatcacata
    17761 gtatggatga tatggcaaag ttagcaaaaa ctttaattgt tatgaatcat ggtagcgtag
    17821 aatttatggg aactcctaga gaagtattta aatcaaatgc tagtaaatta aaagatatag
    17881 gattagatat tccacaagtt ttagagcttg cattgaaatt gagagaaaaa ggctttgaca
    17941 tcagtgagga tatattgact ttagaagaag caaaacagga aatattaaaa gttgtgagag
    18001 gacgaggatt atgttaaaag atataactat agggcaatac tacccaacaa gttctgctat
    18061 tcataaatta gatccaagga taaagcttgt agcgacaatt gtatttatgg tatctatatt
    18121 tgtagtaaat aaattttggc cttatatagt agttttatta tgtttattag ctatgataaa
    18181 attggctaat ataccagtca aatatattgt taagggtgtt aaacctctta aatggattat
    18241 tttgtttaca tttcttataa atattttttt cttaccaggt gatgaaatat ggtcatttgg
    18301 atttttagct attacaaaac agggtctaag acaagcaatt tttatggcaa taagattaat
    18361 atttttagta gttggtacat cattgcttac tttaacaact tcaccaatag aattaactga
    18421 tggaatagag agattgttga atccatttaa gaaaattggt ttgccagttc atgaacttgc
    18481 aatgatgatg acaattgctt tacgttttat tcctacattg ttagatgaga cagacaaaat
    18541 aatgaaggct cagatgtcta gaggtgcaga ttttgaaagt aagaatctta taaatagagc
    18601 aaagaattta gtaccacttt tggtaccttt atttgtaagt gcatttagaa gagctgatga
    18661 attggctatg gccatggagg ccagatgtta cagaggtgga cataatagga ctaagatgag
    18721 agagtctgtt atatcaagaa gggattatat ggcatgtgta tttcaggttg tttatctggg
    18781 agcaattata gctactagat ttatagcaat ttaattaata agttatagat ataatgaggt
    18841 aaagtatgag aaatataaaa attaaaatcc aatacaatgg taagaattat tgtggatggc
    18901 aaaagcaacc tgattcttta gggatacaag gtacaattga aagggctata tatgatataa
    18961 caaaagaaga aaccagtctt atagggtctg gtagaactga ttcaggagtt catgctattg
    19021 gtcagattgc aaattttaaa attaattcag gtataagtat agaaagtata ccaatggcac
    19081 taaatgccaa gttgccaaaa gacatatcag ttatagaagc atgtgaagta aatgatgatt
    19141 ttcactccag atacagtgca aaaggtaaaa catataaata tttagtatat aatagcaagt
    19201 ttagaaatcc aatacttagt gagatatcat atcaagtaaa atatgaactt gattttgata
    19261 aaatgtgttc agaagcaaaa agtttattag gaacacatga ttttaaaggt tttatgagtt
    19321 ctggttcatc tgtaaaagat acagttagaa cgatatatga tattgatata agtaaaaaag
    19381 atgacttgat tacttttgag attagtggta atggtttttt atataacatg gtgaggatta
    19441 ttgttggaac acttgttgat atgggtcgtg gcagaattaa cgaaccattt ttagatataa
    19501 tacaatcaaa aactaggtca agatgtggac atacagctcc agcacaagga ttatttctca
    19561 aaaaagttca ttattaactt gacacaccag gctacatgta ttaaaatata tgagaatgtg
    19621 ttaataagtc cacttgcccc ggacttgaca taaacggtaa aaaaatttta tgaaaagtag
    19681 ttaaggaggg acagttatga aaagttatat agctaagcca gctgatgtac aaagaaaatg
    19741 gtacttagtt gatgctgaag gaaaaacatt aggtcgttta gcaacagaaa ttgcgacagt
    19801 attgagaggt aaacataaac caacatttac tcctcacgtt gatggtggag attttgttgt
    19861 tgtagtaaat gcagaaaaaa tagttttaag tggtaagaaa ttagatcaaa aatattacag
    19921 ataccatact ggttatgtag gtggattaaa agaaatatct tatagagata tgatggataa
    19981 gaaaccagaa gaagttatat cacatgctgt aagcggtatg ttacctaaaa ataaattaag
    20041 aagcagaatg atgactagat taagagtatt tgcaggtgct gagcacactc atgcagctca
    20101 aaatccagaa gttttaaact ttaaataaga ttaataggtg aaaggaggag tttataatgg
    20161 ctaacgttca atattatgga actggaagaa gaaaaagttc tgttgctaga gtaagactag
    20221 ttgcaggtga aggaaatata ttagtaaatg gaagagcatt agaaaattat tttaattatg
    20281 agacattaat aagagatgtt aaacaaccat tagttttaac tggtaatgaa aataaatatg
    20341 atgttatagt aaaagttgaa ggtggaggat tcactggaca agctggagct ataagacatg
    20401 gaatatctag agctttatta aaagctgatt tagatttaag acctgcttta aagaaagaag
    20461 gtttcttaac tagagatgca agaatgaagg aaagaaagaa atacggatta aaagcagcaa
    20521 gaagagctcc acaattctca aaaagataat tacttatttt tttggaaaag caaaatcctt
    20581 tatttactgt tattcagaaa gtattggtat tttaaaaatg ttgggttaag ttaattattg
    20641 atatataata aaataataag atatcttatt aggatgtttt tcattaagaa gaacatcctt
    20701 tttattttga ctatttaata aaaaacatat gctaataaca tttaaatttt atttttataa
    20761 tattgtaatt taaaagttta tataaggtta tatattttaa gatattattt aacaatataa
    20821 attctgaata tacgttatca aaatttataa tataaactaa gtataattta ttgtatagta
    20881 ttgaatgtta gataaacata gtgaattaaa tagggacaga aatataatat cattacataa
    20941 taatatatta ataccaataa ataaagatat atcaatatta aaaaaattta aatgtttgta
    21001 ctaatatatg gaatattata gtgtttttat aagtatacat atacagggtg atgttgtgag
    21061 aaagtacata aaacatataa tttttagttt tgcaatgata tgtctagtag tagtatccat
    21121 atttgaaatt aaaaatattt ctgaagatgt tatcaagtat atgccagtaa caaacaaaac
    21181 tataatttta gatgctggtc atggaggcat tgatccaggt gcattaaata aggataagag
    21241 tacatctgaa aaggatatta acctagcaat aacacttaag cttagagagc ttatagaatc
    21301 aagtggtggc cttgtaatat taactcgaga agatgacagt agcctttata aagaagaaaa
    21361 taataaaact acaagacaaa aatataatga gaatttgaaa aatagaaaag aaataatatc
    21421 taattctaat gccaatatgt ttgtctctat acatcttaat gcatttgaac aatcaaaata
    21481 ctatggagct caaacttttt atcctaaaga caaacaagac agtaaagaat tatcgaagtg
    21541 tattcaagaa gaacttaaaa gagtagtaga taagacaaac aacagagaag tcaaaccaag
    21601 ggatgatata tatcttttga aagaaaataa tattccatca gtactgatag aatgtggttt
    21661 tttatcaaat gaaaaagagt gtaaactctt aactgatgaa acatatcaag aaaaaatagc
    21721 atgggcaatc tacataggaa tacaaaaata tttaagttga aataactata aaaataggtc
    21781 taaatatgta aatagcttgt attaaagcag ttaaaatgca ttaatatagg ctatttttat
    21841 tttgacaaaa aaatatttaa aataaaagtt aaaaagttgt tgacttagaa taatatagat
    21901 gatattatat atgagtgccc aaaaggagca ccaaaataag ac
//
LOCUS       DAYAPA010000064        21647 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.064, whole genome shotgun sequence.
ACCESSION   DAYAPA010000064 DAYAPA010000000
VERSION     DAYAPA010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21647
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.064"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            70..1521
                     /locus_tag="ACGI59_002203"
     CDS             70..1521
                     /locus_tag="ACGI59_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HFG9739989.1"
                     /translation="MDFFNGILTSINNAVVYVNNYLWSYILIIMLVVIGIYFTIKTNF
                     VQFRYFIEMFRLLGDGTANKNAKKEGKISSFQAFCISTSSRVGTGNIAGIAIAVVAGG
                     PGAVFWMWLIALIGSASSFVESTLAQIYKVKDGQAFRGGPAYYMEQGLNKKWMGILFS
                     ILITICYGFVFNAVQANTVSLAFNNAFGISKMAMGIGLGILTAIVIFGGVHRVAKVSE
                     IIVPVFAGLYILVALAIVVMNITEIPSVIALIVESAFDFKGMAIGTFMGVVMTGVKRG
                     LFSNEAGMGSAPNAAATAHVTHPVKQGLIQSLGVFTDTIIICSCTAFMVLLYSGYSSS
                     GATGIELAQEALTHHIGPVGNIFIAVCIFLFAFSSIVGNYYYGESNMEFMSGSKTKLN
                     VFRVFVVGMVLFGSLTQVDVVWNLADLFMALMAIINLIAIALLGKYAFIALKDYTSQK
                     KSGIKDPVFIADEIEGLEKVSEWHRELSEEHLG"
     gene            complement(1740..2201)
                     /gene="ribE"
                     /locus_tag="ACGI59_002204"
     CDS             complement(1740..2201)
                     /gene="ribE"
                     /locus_tag="ACGI59_002204"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HFG9739990.1"
                     /translation="MIYEGKLIGKDLKIGIINSRFNEFITSKLLSGAEDCLLRHDVSP
                     ENIEIVWVPGAFEIPLVAQKMAKSGKYDAIICLGCVIRGATSHYDYVCSEVSKGIAKV
                     SLDSELPVIFGIVTTENIEQAIERAGTKAGNKGYDCAMNALEMANLFKSLN"
     gene            complement(2306..3499)
                     /locus_tag="ACGI59_002205"
     CDS             complement(2306..3499)
                     /locus_tag="ACGI59_002205"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HFG9739991.1"
                     /translation="MFNTIEEAIEDIKNGKMVIVVDDESRENEGDLLMAAEMATPESI
                     NFMATYGRGLICLPATEKKFKSLNIPLMVRENTDTFQTAFTVTIDGADTLTGISAYER
                     AETVKLFCDENSTSKDFKTPGHIFPLIAKTGGVLVRDGHTEASVDLARLAGFKEIGLI
                     CEIMKDDGTMARVDDLMIFKEKHNLKIITIKDLIEYRKINETTIEKVSSAFLPTKYGN
                     FEIIGYRDTYSNEEHIALTYGNINIENTLVRLHSECLTGDVFHSLKCDCGLQLESSMK
                     KIVENGSGVLIYMKQEGRGIGLLNKIKAYKLQEEGYDTVEANLMLGFEEDMRDFYMAA
                     QILKNLNIKSINLLSNNPDKINQLEKYGIKIENRIPINEEINDFNKLYLKTKKDKMGH
                     LLDII"
     gene            complement(3522..4187)
                     /locus_tag="ACGI59_002206"
     CDS             complement(3522..4187)
                     /locus_tag="ACGI59_002206"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HFG9739992.1"
                     /translation="MFTGIVEEVGILRKITANGKSGKVTILSNKILDGTNLGDSIAVN
                     GVCLTVSNLGKNEFTADVMMETIRSTNLGLLNANDKVNLERAMSLSSRFGGHIVTGHV
                     DGKGTICKFEKDENAVLVSIRPDKKLLSSMILKGSVAIDGVSLTISYLDDEIFKVSII
                     PHTKINTILLTKNVGDFVNLESDVIGKYVNNFMANNYKELNSNSNNHKSNIDKDFLFK
                     NGF"
     gene            complement(4221..5333)
                     /gene="ribD"
                     /locus_tag="ACGI59_002207"
     CDS             complement(4221..5333)
                     /gene="ribD"
                     /locus_tag="ACGI59_002207"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HFG9739993.1"
                     /translation="MNFVNQKEKDIYYMKKAIELAKNGEGFVNPNPLVGCVIVKASNI
                     IGKGYHEKFGSNHAEVNAINSAKQSLKDSTLYVNLEPCSHYGKTPPCVDKIIQNKIKR
                     VVISTLDPNPLVCGNGVKKLKDNNIDVTVGILEDEARDLNEAFFYHIKNKRPLCIVKS
                     AVSLDGKIATKSLESKWISNESSRYLTHKYRNKYQSIMVGINTVLNDNPLLTCRLNQE
                     KVSHPTRIVIDTHLKLPLNSNLVKDKTSKTIVFTCCKESIKLSMLKENNVETIISPSK
                     NNLVDLEFVMYKLGELNIDSVLVEGGATLNDSLFRNKLVDKVKLFLSPKIIGGKDAPT
                     FVSGEGINHLSDSTQLTINNVTLIDGDILIESDVLN"
     regulatory      complement(5465..5586)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            5775..8258
                     /gene="recQ"
                     /locus_tag="ACGI59_002208"
     CDS             5775..8258
                     /gene="recQ"
                     /locus_tag="ACGI59_002208"
                     /EC_number="5.6.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HFG9739994.1"
                     /translation="MNKKLLEILSKYYGYTSFRKGQESIINSILSEKDVLAIMPTGGG
                     KSICYQIPALILDGMTIVISPLISLMKDQVDALKTMGISAAFINSSLSSKEFNEILNN
                     IRKNNYKILYIAPERLDAQEFIELINNNNVSQVAIDEAHCVSQWGHDFRLSYRRISDF
                     IKNLPKRPIVTAFTATASEEVRTDIINLLCLENPDYYITGFDRENLSINIVKSSSKNK
                     YILDYIQNHKNESGIIYVATRKEVENIYNGLSKRNISVSKYHAGLSKNERSKNQEDFI
                     NDNVDIMVATNAFGMGIDKPNIRWVIHYNMPQSIENYYQEIGRAGRDGEKSECVLLFS
                     PGDVHIQKYLIDIGVENPERKLFQHKKLQYMIDLVYSNSCYRKTILSYFGENYIDNCN
                     NCSNCLVEGEIVDKTIDAQKVISCVARMKRSYGVTTIVDVLRGSKNKKILQLGLNTLS
                     TYNIMRDYSSEDLKNFVNTLVSHGFLDLVETLGNGNRGSFPTIRLNEMSMKVLKGEVK
                     VEFKEIVMTKSLEVEDELYNALRELRHSIASEERIAPYMVFGDGTLRAMSSSYPINKE
                     EMLDISGVGEIKYQKYGRDFEAVIKEYVEKNNINKKIDEKNVDSKNVNSDFLEVNTDK
                     VLYEKLLVIREEYATKEKLPAYMILANKALKEISGRYPLDEEQLKDISGIGGVKFEKY
                     GASILAEVNKYVKENNINVIWEKKGKRKLILDGESRKNNEIALDLLNQGKDIQSISQE
                     IEVSLSTIIGYVHDYVKDGHSISFELGLENFYNEDKKKMILEVCEKVGYDNLNNIKRK
                     LPDSIKYENIRAVILDSYLENLKNNIIAE"
     regulatory      8347..8458
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            8600..9901
                     /gene="thiC"
                     /locus_tag="ACGI59_002209"
     CDS             8600..9901
                     /gene="thiC"
                     /locus_tag="ACGI59_002209"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HFG9739995.1"
                     /translation="MNYTTQMDAARKGIITKEMEIVSQKEQVDVNELRELIANGQVVI
                     PANKNHKSLSAEGVGKNLRTKINVNLGISRDCKDIEKELEKVRVAIDMKAEAIMDLSN
                     YGKTREFREKVVEMSPAMIGSVPMYDAVGYLEKELKDITEEEFLNVIRQHAIDGVDFI
                     TIHAGLTRSVCQKIKNHERLTHIVSRGGSLLFAWMELNNKENPIYTNFDKILDICEEY
                     DVTLSLGDACRPGCIKDSTDGVQIQELVVLGELTKRAWERNVQVMIEGPGHMAIDEIE
                     ANVVLEKRLCHGAPFYVLGPLVTDIAPGYDHITSAIGGALACAKGVDFLCYVTPAEHL
                     RLPNLDDMKEGIIAAKIAAHAGDIAKNVKGAREWDNKMSKARADLDWCEMFRLAIDPE
                     KAKRYRDESTPTHEDSCTMCGKMCSMRTVKKILNNEELNLI"
     gene            9955..10149
                     /gene="thiS"
                     /locus_tag="ACGI59_002210"
     CDS             9955..10149
                     /gene="thiS"
                     /locus_tag="ACGI59_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HFG9739996.1"
                     /translation="MKVNGKEIEFEKDLTVIDLLNKYNLKSDRVVVEVNLEIIEESNY
                     NTYVLKDEDIVELISFIGGG"
     gene            10142..10945
                     /gene="thiF"
                     /locus_tag="ACGI59_002211"
     CDS             10142..10945
                     /gene="thiF"
                     /locus_tag="ACGI59_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine biosynthesis protein ThiF"
                     /protein_id="HFG9739997.1"
                     /translation="MVKIKIFVNELLTDVEENTTAYKVRDSYNNKCDVLVLNGYPIKT
                     DMTLCENDKLTLIQKGVKPSLDELESLLIARHTPNIHNKLKKGKVAILGLGGLGSNIA
                     ISLARIGVGELLLIDYDVVEPSNLNRQQYFIDDIGKLKTDAMIENIKKINPFIKLNKR
                     DIFLNKNNMDTIKDSDLIIEAFDDASCKAQVCNYVLINLKDKYLIASSGMAGYYDSNI
                     ITTKKIKDRFYICGDFVNEAKFGEGLMAPRVAICANHMANLATQILIDM"
     gene            11008..11778
                     /locus_tag="ACGI59_002212"
     CDS             11008..11778
                     /locus_tag="ACGI59_002212"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HFG9739998.1"
                     /translation="MDKLVLGGHEFNSRLLVGTGKYGSNNILPEVIKESGSEIITMAL
                     RRVDLDNKQENILTYIPKEMTILPNTSGATNAEEAVRIARISRKMGCGDFIKIEVISD
                     TRYLLPDNEETIKATKILADEGFIVLPYMTPDLYAGRRLIEANAAAVMPLGAPIGSNR
                     GLQMKEMIRIMIDELDIPIIVDAGIGKPSQAMEAMEMGADAVLVNTAIASAGDPVQMA
                     RAFKLAVEGGREAYIAKTGNVSEFANASSPLTGFLGNL"
     gene            11795..12898
                     /gene="thiH"
                     /locus_tag="ACGI59_002213"
     CDS             11795..12898
                     /gene="thiH"
                     /locus_tag="ACGI59_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HFG9739999.1"
                     /translation="MSFYDVIEKYRDFDFDGYLNNVTDNDVLRSLSKDKLEDFDILNL
                     LSKTAVKHLEDMAQKAHKLSVQYFGKTVCLYTPMYIANYCVNQCVYCSYNIKSGIKRK
                     KLTMDEIREEGEVISKEGFKHLLVLTGESSFHSSVEYIGEAIEILREKFPSIGIEVYP
                     MEVEEYKYIVDKGVEGLTVYQETYDEEIYKRVHIKGPKSNYKFRLDAPERGAKAGMRT
                     LSIGALLGLNDFRKETFFTILHGKYLKTKYPHIELSYSTPRMRPFKGCFEELVDISDT
                     DLVQAMVCMRLFDPHAAINISTRENLEMRSHIIPLGVTKLSAGVSTDVGGHSQDEHDT
                     AQFKINDESTVKDVEKMLNSIGYQHVFKDWERF"
     gene            12899..13588
                     /locus_tag="ACGI59_002214"
     CDS             12899..13588
                     /locus_tag="ACGI59_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HFG9740000.1"
                     /translation="MYLITNRKLCSEERYLEVIKESILSGVENIIIREKDLEYQELRK
                     LYMKIKTKINCIDFQEQISDESLKTNINQKEFRNKFKVNFIINSNIEFFEKVDCQGIH
                     LPFKLFLNLIENKYNFNENKILGLSLHKVEEVDYLEKLIRNQNIKIDYITLSHIYETK
                     CKEGLNPKGIELLKEAKKITDIKIIALGGILPSNVKETLKYCDDFAIMSTIMRSKDIK
                     KTISNYNEKLN"
     gene            13776..15170
                     /locus_tag="ACGI59_002215"
     CDS             13776..15170
                     /locus_tag="ACGI59_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFG9740001.1"
                     /translation="MTDSKKKRFIIPHTFTIIFLLIVLMAILTWIVPSGEFSREDVEG
                     RMVVVPGTYQSVESNPQGFADVFKAPIEGFIDSAEVVGFVLIVGGAFGIVNRTGAIEA
                     GIASVISKFKGKELLIIPISMILFGLGGTTFGMAEETLPFYMIFVPLMTSLGYDSLTA
                     IAIVFIGAAAGAAASTINPFSVGIAQALAQLAPGSGVTYRVVIFIAYILISIAFVMYY
                     AKKVKENPESSIVYEIDKNNKSILNQTSTEIKEFTFKEVSVLAIFGIGMAIMIWGVLS
                     QGWYIPEISMCFAAIGVLSGLVGRLSQIEISESFIDGAKDLASAALAIALARGIVIIA
                     QNGFIIDTILNSAAAFLGGLPKVIFVYLSFFLEAALAFLIPSSSGLASLTVPVLAPLG
                     DLVNISAQVIVTAYQFGVGLTNFITPTSGVLMGALAVAHIPFSKWVRFIMPILLILTA
                     VSLVFLTIGIYIGF"
     gene            15687..17054
                     /locus_tag="ACGI59_002216"
     CDS             15687..17054
                     /locus_tag="ACGI59_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HFG9740002.1"
                     /translation="MGLKEFDFIEESIDMLRTMSPTLETISDEIEEYFENILDEKNQE
                     YINVTSRIKSESSLREKIIRNRYLKKYGEASNLIHNVSDLIGLRIECRFIEDENKIYR
                     LLRRYFNKTDDKINYYNKENKNIKLKLSERQPNKQKNGFEIYKIDGVFAYLDREVKFE
                     LQIKSLVNVFWSEIEHKIIYKNNTYLLADKFIKDMMDSIKNNLTMIDNQLLSIYKNFH
                     SGKSFNMKVSKKEIEKLFAKLVYDAFSEKMNKSIGFVVDFKKPCETILNYSFNKQDIS
                     DEVLGNFMLDEFARLNEIVNKDIDFNEQIEFEREPTFDDKFCKDLGNHFRSRLNTEFP
                     WNLFFRILFEIEPYNNTKDFENFVEFIKENVLLEENREQLLCQFEEDSKLIIEDIYDC
                     IFNSISEIDSVEILYNYNLEKINFTSSEIINCICREYECYGEYLEEKEKLMNTFKEKI
                     IQIFE"
     gene            17079..17687
                     /locus_tag="ACGI59_002217"
     CDS             17079..17687
                     /locus_tag="ACGI59_002217"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HFG9740003.1"
                     /translation="MRHFSNIYELSKIILEEAICIEKTGVILAGGKNSRMGRDKAFLE
                     LHDKLFIEIAIEAFKNFDELIIISNNEELYSKYDIKVYNDIVKDVGPIGGIYTALEYA
                     KYDIVTIACDMPYLNNQIVERIANKMDDKSVISVTNGKLQPLCSGYKKSIINKVSLCI
                     RENDLKLRSFIDKIDKSYIYFDEEDLFLNVNTVDEYKKLTKV"
     gene            17735..18106
                     /locus_tag="ACGI59_002218"
     CDS             17735..18106
                     /locus_tag="ACGI59_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFG9740004.1"
                     /translation="MNLLKCDSWAVILNNSDKESKAYKILDELKRNMYKVVAIDEEKK
                     PIEGIDVYECLKDVPHNIDVVAIIDKQSKMDVILEEVELLDIQNMWFEKGSFSEMMIK
                     KTKDLKLNIEYNLSLYDELTR"
     gene            18285..18623
                     /locus_tag="ACGI59_002219"
     CDS             18285..18623
                     /locus_tag="ACGI59_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFG9740005.1"
                     /translation="MLIVTTEKVEGKKISKVLGLVRGSTIRAKHVGKDIGASFKNLVG
                     GELTGYNEMLTEARQIAIGRMVEDAEAKGANAVIAFRLSSASVMQGAAEMLAYGTAVV
                     LEDDNSILEK"
     gene            19089..19592
                     /locus_tag="ACGI59_002220"
     CDS             19089..19592
                     /locus_tag="ACGI59_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HFG9740006.1"
                     /translation="MFNVAIITLSDKGYEGKREDITGKKLTEFVENTGVYKVTEYVLI
                     KDDKEMLKENIIRLCNSNKIDLILTNGGTGFSKRDITPEATKEVLEKEIPGLSEYMRM
                     KSTEITKKAILSRGVSGIRNNSIIINLPGSPKGAVENLSFIIDVLDHGIEVLRGEATE
                     CATKKSE"
     gene            19639..20598
                     /gene="moaA"
                     /locus_tag="ACGI59_002221"
     CDS             19639..20598
                     /gene="moaA"
                     /locus_tag="ACGI59_002221"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HFG9740007.1"
                     /translation="MIDKYGRKIDYLRISLTDKCNLRCVYCMPPDVKFDKNYINENLS
                     FEDYKFIIKAMAEQGITKIRFTGGEPLLYPKLNELIKFTKEECGINNIGMTTNAIGLS
                     DRIFELEKCGLNKVNISLDSLKEYKYKSITRGGNLKDVLKSIESCLNLGIKVKLNCVA
                     ISGFNDDELKDFMFLTVKSPIDVRFIELMPLGEANRVYKKGYFNVKEMIEKIYGLYKV
                     KNDEKSTAEYYMFKGAKGRIGIITPLSCSFCNTCNRIRLTSSGAIKLCLHSKEEIDIK
                     PFLHKPLLFRESMKDIIKQKPEKHHLIENKCSDTNRRMYEIGG"
     gene            20602..21072
                     /gene="moaC"
                     /locus_tag="ACGI59_002222"
     CDS             20602..21072
                     /gene="moaC"
                     /locus_tag="ACGI59_002222"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HFG9740008.1"
                     /translation="MLTHINEKGYAKMVDISEKDDTKRTAVATATITLSKEALEKVKN
                     GQIKKGDVLSVAQVAGIMGAKNTSSNIPMCHQIKLVGCDLEFDVDDSMSAIKIYATCK
                     SLGKTGVEMEALNACSLAALTIYDMCKAVDKKMVISNIHLVEKTGGKSGDFKFE"
     gene            21108..21539
                     /locus_tag="ACGI59_002223"
     CDS             21108..21539
                     /locus_tag="ACGI59_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HFG9740009.1"
                     /translation="MAKVVSINISEKKGVIKVPVQCAELKINHGIVGDAHAGNWHRQI
                     SLLGKESIDKMKMKGFDQLKYGDFAENVTTEGIEVFSLPVGTKLRIGQCEVEVTQIGK
                     KCHNGCEIKKLTGDCVMPREGIFVKVLKEGIINVNDSIDVI"
BASE COUNT         8343 a         2338 c         3747 g         7219 t
ORIGIN      
        1 gaattattca tttttttgat tttaagattt attgagaaaa caaaactaaa taggggggta
       61 attaatataa tggatttttt caatggtatt ttaacgagta ttaacaatgc agtagtatat
      121 gtaaacaatt acttatggtc gtatatctta attataatgt tagttgtaat tggtatttac
      181 tttactatta aaacaaattt tgtacaattc agatatttta tagaaatgtt tagacttctg
      241 ggtgatggta cagcgaataa aaatgcaaaa aaggaaggaa aaatctcttc atttcaagca
      301 ttttgtataa gtacatcttc aagagttgga actggaaata tagctggaat agcaattgca
      361 gtggtagcag gaggacctgg tgctgtattt tggatgtggc ttatagcatt aattggttct
      421 gcatcaagct ttgttgagag tacattagct cagatttaca aagtgaaaga tggtcaagct
      481 tttagaggtg gaccagcgta ttatatggaa caaggtctaa ataaaaaatg gatgggtatt
      541 ttattctcaa tacttataac tatatgttat ggttttgtat ttaatgcagt tcaagcaaat
      601 actgtttcac ttgcatttaa taatgcattt ggaataagta aaatggctat gggaataggt
      661 cttggaattt taactgcaat agttattttt ggtggagtgc atagagttgc aaaagtttct
      721 gaaataatag ttcctgtatt tgcaggatta tatatattag ttgctttggc tatagttgta
      781 atgaatataa cagagatacc aagtgttata gcattgatag ttgaaagtgc atttgatttt
      841 aaaggaatgg cgattggtac atttatggga gttgtaatga ctggagtgaa gagaggtcta
      901 ttttctaatg aagcaggtat gggtagtgct cctaatgctg ctgcaacagc acatgtaact
      961 catcctgtaa aacaaggttt aattcaatct ttaggtgttt ttacagatac tataataata
     1021 tgtagttgta cagcattcat ggtattatta tattcaggtt atagtagtag tggagcaact
     1081 ggaattgaat tagctcaaga agctttaact catcatatag gaccagttgg aaatatattt
     1141 atagcagttt gtatattctt gtttgcattt agttctatag ttggtaatta ctattatggt
     1201 gaatcaaata tggagtttat gagtggaagt aagactaaac ttaatgtatt tagagttttt
     1261 gttgtaggta tggttttatt tggttcactt actcaagtgg atgtagtttg gaatcttgca
     1321 gacttattta tggcattgat ggcgataata aacttaatag caatagcact tttaggaaaa
     1381 tatgcattta ttgctttaaa agattataca agtcaaaaaa aatcaggaat aaaagaccct
     1441 gtgtttattg cagatgaaat agaaggccta gaaaaggtat cagagtggca tagggaatta
     1501 tctgaggaac atttaggata aaaaatagta taaaattata aaaccatttt gaaagactag
     1561 agtatgtata tactttatat gaatataata ttgaattaat ttatattact ggaattttta
     1621 attccagtaa tattttttta ttatataatt tagctttatt aaaagttttt attattaaaa
     1681 atagcttatt tatcgtaaag tgaatgatat tgggttttaa ttattatgaa tgttagtatt
     1741 taatttaaag acttaaataa atttgccatt tctagcgcat tcatagcaca atcataacct
     1801 ttatttccag cttttgtacc tgctctttca atagcttgtt caatattttc tgtagttacg
     1861 attccaaata taacaggtaa ttcactgtct aaagatactt tggctatccc tttagagact
     1921 tcactacata cataatcata atgagaagta gcccctctta taacacagcc taaacagatt
     1981 atagcatcat acttaccact tttagccatt ttttgagcaa caagaggaat ctcaaaagct
     2041 ccaggtaccc aaactatttc tatattttct ggagaaacgt catgtcttaa aagacagtct
     2101 tcagctcctg ataaaagttt tgatgttata aattcattaa aacgagaatt tattatacct
     2161 atttttaaat cttttccaat aagttttcct tcatatatca tttttacttc ctccaatttt
     2221 atttatgtta tagatttata tttataaata tctttaattt aattataaaa aactacttag
     2281 tagaaaaata tcatcatata atcatttata taatatctaa aagatgtccc attttatctt
     2341 ttttagtttt taaatatagc ttattaaagt cattaatttc ttcattaata gggattctat
     2401 tttcgatttt tattccatat ttctctaatt gatttatttt atctgggtta ttacttaaaa
     2461 gatttatact ttttatgttt aaatttttta atatttgagc agccatatag aaatctctca
     2521 tatcttcttc aaatccaagc attaaatttg cttcaacagt atcgtaacct tcttcttgaa
     2581 gtttataagc cttaatttta tttaaaagac caatacctct accttcttgc ttcatataaa
     2641 ttaaaactcc acttccattt tccacaattt tcttcataga agattctagc tgtaatccac
     2701 aatcacattt tagagagtgg aacacatctc ctgttaaaca ttcagaatga agtctaacta
     2761 gagtattttc tatatttata tttccataag taagggcaat gtgttcttca tttgaataag
     2821 tatctctata tcctatgatt tcaaaatttc catattttgt cggaaggaaa gcactagata
     2881 ctttttctat agtagtttca tttattttgc ggtattctat aaggtctttt atagtgatga
     2941 tttttaaatt atgcttttct ttgaaaatca ttaaatcatc aactcttgcc attgtaccat
     3001 catctttcat tatttcacag ataagaccaa tctctttaaa acctgcaagt ctagcaaggt
     3061 ctactgaggc ttctgtatgt ccatccctga caagaacccc accagttttt gctattaaag
     3121 gaaagatgtg accaggcgtt ttgaaatctt tacttgtaga attttcatca cagaataatt
     3181 ttacagtttc agctctttca tatgcagata taccagttag agtatctgct ccatctatag
     3241 taacagtaaa ggctgtttga aatgtatctg tattttctct aaccataaga ggtatattaa
     3301 gacttttaaa ttttttttca gtggcaggta gacaaattag acctctacca tatgtagcca
     3361 taaaatttat tgattcagga gtagccattt cagctgccat caataaatct ccttcattct
     3421 ctctactttc atcatcaacc acgattacca ttttcccatt ttttatatct tcaatagctt
     3481 cttcaatagt attaaacatg taattcactc ctttattatt tttaaaaacc atttttaaat
     3541 aaaaaatctt tatctatatt tgatttatgg ttattactat tagaatttaa ttccttgtag
     3601 ttattagcca taaaattatt aacatacttt ccaattacat cgctttctaa attaacgaaa
     3661 tctccaacat tctttgtcaa gagaatagtg ttaattttag tatgaggtat aatagaaacc
     3721 ttaaatattt catcatctaa atatgatata gttaaactta caccatcaat agcaacagaa
     3781 cctttgagta tcattgagga taaaagtttt ttatcaggtc gtatacttac taaaactgca
     3841 ttttcatctt tttcaaattt gcagatagtt cctttaccat caacatgacc tgttactata
     3901 tgaccaccaa atcttgaaga aagactcata gctctttcaa gatttacctt gtcatttgca
     3961 tttaataaac ctaaatttgt ggaacgaatt gtctccatca taacatcggc tgtaaattca
     4021 ttttttccga ggtttgaaac agttagacaa actccattta cggctatact atctccaaga
     4081 tttgtgccat ctaatatttt atttgatagt atggttactt ttccactctt accattagca
     4141 gtaatttttc taagtattcc aacttcttct actatgccag taaacatcct aacctcctat
     4201 atgaatgtat taacaaaaat ttaatttaaa acatctgatt ctataagaat atcgccatct
     4261 atcaaagtta cattattgat tgttaactgt gttgaatctg acaaatgatt tattccttct
     4321 ccagaaacga atgtaggtgc atctttacca cctataatct tgggtgatag aaataatttg
     4381 accttatcaa ctaacttatt tctaaagaga ctgtcattga gagtagcacc accttcaact
     4441 agaacagaat caatgtttaa ctctcctaat ttatacatta caaactccaa atctactaag
     4501 ttgttttttg aaggagaaat tattgtttca acattatttt cttttaacat agataatttt
     4561 atactttctt tgcaacatgt gaaaactata gttttactgg ttttatcttt aaccaaatta
     4621 ctattaagtg gcaactttag gtgtgtatct attactattc ttgtaggatg agacactttt
     4681 tcttgattga gcctacaagt tagtaaagga ttatcgttca aaactgtatt tatgccaacc
     4741 attatacttt ggtatttatt tctgtattta tgagttagat accttgatga ttcattagat
     4801 atccattttg attctagtga ttttgtagca attttaccat ctagacttac tgctgatttt
     4861 acaatacaaa gaggtctttt gttttttata tgataaaaaa atgcctcatt taaatctctt
     4921 gcttcatctt ctaatattcc cacagtaaca tcaatattgt tatcttttaa tttttttact
     4981 ccattaccac aaacaagtgg atttgggtct aatgtagata ttacaactct cttaatctta
     5041 ttttgtataa ttttatctac acaaggaggc gttttcccat aatgagaaca tggttctaag
     5101 tttacataaa gagtagagtc ttttagactt tgtttagcac tgtttatagc atttacttca
     5161 gcatgattag agccaaattt ctcatgatat cctttgccta ttatattact agctttaaca
     5221 attacacacc caactaatgg atttggattt acaaagcctt ctccattttt agccaattca
     5281 atagcttttt tcatataata aatatctttt tctttttgat tcacaaaatt cacaatatgt
     5341 acacctcctg tttaattgga taatttttaa acaaaaaacc ctgagaatta actcagggtt
     5401 tagaataatc tacaataaat gtttaataaa tgtagaacaa aattgctcta caaaaacact
     5461 cattaaatat cttcttccat ccagacttta ctgtcggctt tggaatttaa ccaaatcaac
     5521 caagttttac cttggctcgc gggctatacc gccgattggg aatttcaccc taccccgaag
     5581 attaagtatt aaatttatat ttagaatgta tcataaatta ttttactttg caaactattt
     5641 ttttaaaaaa tatatgttaa tatataatat agaatgtcta taaactttga aataccacga
     5701 ttgtgaatat aaaaataaaa atttgaacat aatcaggaaa gaagtctaat taatttaaat
     5761 taggagagta atatatgaat aaaaaacttt tagaaatact atcaaaatac tatggatata
     5821 caagttttag aaaaggtcaa gaatctataa taaactctat cttaagcgaa aaagatgtgt
     5881 tagctattat gccaacagga gggggaaaat caatatgtta tcaaattcca gctttgattt
     5941 tagatggaat gacaatagta atttctccac ttatatcact tatgaaagac caagtagatg
     6001 ccttaaagac tatgggaatt agtgctgctt ttataaatag ttcattaagt agcaaagaat
     6061 ttaatgaaat attaaataat attagaaaaa ataattataa aatactctat attgctccag
     6121 agagattaga tgcacaagag tttattgagc taataaataa taacaatgta agtcaagttg
     6181 cgatagatga agcacattgt gtatctcaat gggggcatga ttttaggttg agttatagaa
     6241 ggatttctga ttttataaaa aatttgccta aaagacctat agttacagca tttactgcaa
     6301 cagcttcaga agaggtaaga actgatataa taaatttact gtgtttagaa aatccagatt
     6361 attatataac tggatttgat agagagaatt tatcaataaa tatagtaaaa tcatctagta
     6421 aaaataagta tattttagat tatattcaaa atcataaaaa tgagtctgga attatatatg
     6481 ttgcaactag aaaggaagtt gaaaatatat ataatggttt atctaagaga aatatatctg
     6541 tgtcaaaata tcatgcaggt ttatctaaaa atgaaagaag taaaaatcaa gaagatttta
     6601 taaatgataa tgtggatata atggtagcaa caaatgcatt tggaatgggt attgataaac
     6661 ctaatataag atgggttata cattacaata tgcctcaaag tatagaaaac tactatcaag
     6721 aaataggtag agctggtagg gatggagaaa aaagtgagtg tgtactttta ttttctccag
     6781 gagatgtaca tatacaaaaa tatttaattg atattggagt tgaaaatcct gaaagaaaat
     6841 tatttcagca taagaagctt caatacatga ttgacttagt ttacagtaat tcatgctata
     6901 gaaagacaat attgagttat tttggagaaa attatataga caattgcaat aattgtagca
     6961 attgtttagt agagggtgaa atagtagata aaactataga tgcacaaaaa gtcatttcat
     7021 gtgtagctag aatgaaaaga agttatggtg ttactactat tgtagatgtg cttagaggtt
     7081 ctaagaataa aaagatttta cagcttggat taaatacttt gtctacatat aacattatga
     7141 gagattattc tagtgaagac ttaaagaatt ttgtaaatac attggtttct catggttttt
     7201 tagatttagt tgagactcta ggcaatggaa atagaggtag ttttcctact ataagattaa
     7261 atgagatgtc tatgaaagtt ttaaaaggtg aagtgaaagt agagtttaaa gaaattgtta
     7321 tgacaaaatc attggaagta gaggatgagt tatataatgc tttaagagaa cttagacatt
     7381 caattgcaag tgaagaaagg atagctccat atatggtatt tggagatggt acacttagag
     7441 ccatgtctag tagttatcca attaataaag aagaaatgct agatatctct ggagtaggag
     7501 aaattaaata tcaaaaatat ggtagagatt ttgaagcagt aattaaagaa tatgtcgaaa
     7561 aaaataatat aaataaaaaa atagatgaaa aaaatgtcga ttcaaagaac gttaatagtg
     7621 atttcttaga ggttaataca gataaagttt tatatgaaaa actgttagtc attagagaag
     7681 aatatgcaac aaaggaaaaa ttacctgcat atatgatttt agccaataag gctttgaaag
     7741 aaataagtgg aagatatcca cttgatgaag aacaattaaa agatatttct ggcataggtg
     7801 gagtaaaatt tgaaaaatat ggagctagta ttttggcaga ggttaataaa tatgtaaaag
     7861 aaaataatat taatgttatt tgggagaaaa agggcaaaag gaagttaata ttagatggag
     7921 aaagtagaaa aaataatgaa atagcattag atttgctaaa tcaaggaaaa gatatacaaa
     7981 gtatcagtca agaaatagaa gtttcactgt caaccattat tggatatgtg catgactatg
     8041 taaaggatgg acatagtata tcatttgaat taggactaga aaatttttat aatgaagata
     8101 aaaagaagat gattttagaa gtttgcgaaa aagttggcta tgataattta aataacataa
     8161 aaagaaaact tccagattca ataaagtatg aaaatataag agctgtgata cttgactctt
     8221 atcttgaaaa cttaaaaaat aatattatag cagagtaatt tttaacttaa taaaaaataa
     8281 aatttgtatt attgtgttga actaataatt ttgctgtgtt atactgaatt tgtaaaaata
     8341 atgaaaaatt agagctaggg gcgctagaat tggctagctg agagataaag ccgtaactta
     8401 caaaccctaa taacctgatc aagataatgc ttgcgaaggg aagcttttta atataatttt
     8461 attatataaa taggcaaatt attcaatgtt aggagaaatt tgtctaagtg tatatacgat
     8521 tataagagtg ctttccaaac gaggaaaagc acttttttta ttttcaaaaa ctataacttt
     8581 aatcttggag gattgtaata tgaactatac aacacaaatg gatgctgcta gaaaaggcat
     8641 aataactaaa gaaatggaaa tcgtttctca aaaagaacag gtagatgtaa atgaattaag
     8701 agaattaata gcaaatggac aagttgttat accagctaat aaaaatcata aatctttaag
     8761 tgcagaaggt gtaggtaaaa atttaagaac aaaaataaat gtaaatctag gaatttcaag
     8821 agattgtaag gatatagaaa aagaacttga aaaagttaga gtagcaatag acatgaaagc
     8881 tgaagctata atggatttaa gtaattatgg caaaactaga gaatttagag aaaaggttgt
     8941 tgaaatgtca cctgcaatga taggttcagt acctatgtat gatgcagtag gatatttgga
     9001 aaaagaatta aaagatataa cagaagaaga gtttttaaat gtaataagac aacatgctat
     9061 tgatggagta gactttatta ctatacatgc aggtcttaca agaagtgttt gccaaaaaat
     9121 aaaaaatcat gagagattga ctcatatagt atctcgtgga ggttctttat tgtttgcatg
     9181 gatggaatta aataataagg aaaatcctat ttatactaat tttgataaaa tattggatat
     9241 atgtgaagaa tatgatgtta cattgagttt aggagatgct tgtagaccag gttgtataaa
     9301 agattctact gatggtgttc aaattcaaga acttgtagta ttaggtgaac ttactaaaag
     9361 agcatgggaa agaaatgtac aagtaatgat agaaggtcct ggacacatgg ctattgatga
     9421 aatagaagca aatgtagtat tagaaaagag attatgccat ggagcacctt tttatgttct
     9481 tggaccattg gttacagata tagcaccagg ttatgaccat ataacaagtg caataggagg
     9541 agctttagca tgtgccaaag gagtagattt tttatgttat gtaactccag cagaacattt
     9601 aagattgcct aatttagatg atatgaaaga gggtataata gctgcaaaga tagctgctca
     9661 tgcaggagat atagctaaaa atgttaaagg tgctcgtgaa tgggataata agatgagtaa
     9721 agctagagct gatttagact ggtgtgagat gtttagactt gcaatagacc ctgaaaaagc
     9781 taagagatac agagatgaat caactcctac tcatgaggat agttgtacta tgtgtggaaa
     9841 aatgtgctca atgagaacag ttaagaaaat attaaacaat gaggaactta atctaatata
     9901 atttttggct agtatgactg gaataaaaat aagctgaaaa gaggaggtga tttagtgaag
     9961 gttaatggga aggaaattga atttgaaaaa gatttgactg ttatagattt attaaataaa
    10021 tataatttaa aaagtgatag agttgtagtt gaagtgaatt tagagataat tgaagaatct
    10081 aactacaata catatgtatt aaaagacgag gacattgtag agcttattag ctttattgga
    10141 ggtggttaaa ataaaaatat ttgtaaatga attattaaca gatgttgagg aaaatacaac
    10201 tgcttataaa gttagagata gctataacaa taaatgtgat gtattggtgt taaatggata
    10261 tccaattaaa acagatatga ccctttgtga aaatgataaa ttaacactta tacaaaaagg
    10321 agttaagcca tcacttgatg aattggaaag cttgttgatt gctagacata ctccaaatat
    10381 acataataaa ttaaaaaaag ggaaagttgc tatattggga ttggggggac ttggttccaa
    10441 tatagctatc tctttagcta gaataggagt gggagaactt ttacttatag attatgatgt
    10501 tgtagaacca tctaatttaa atagacagca gtattttata gatgatatag gaaaactaaa
    10561 aactgatgcc atgatagaaa atataaaaaa aataaatcca tttataaaat taaataaacg
    10621 agatattttt ttaaacaaga acaatatgga tactattaag gattcagatt taataattga
    10681 ggcctttgat gatgcttcat gtaaagcaca ggtatgtaat tatgtattaa taaatttaaa
    10741 agataaatat ttaatagcat catctggtat ggcaggttac tatgattcaa atataataac
    10801 tactaagaag ataaaagaca ggttttatat atgtggggat tttgtgaatg aagctaaatt
    10861 tggtgaaggg cttatggctc caagggttgc catctgcgca aatcatatgg caaatttggc
    10921 aacgcaaata cttatagata tgtaaatatt aaaattagat ataaaaaaga ttgaatatta
    10981 aaaaaattta acgtggaggg aaataatatg gataaattgg tacttggtgg acatgaattt
    11041 aatagcagat tattggttgg aacaggtaaa tatggttcaa ataatatatt gcctgaggta
    11101 ataaaagaaa gtggaagtga aataataaca atggcactta gaagagtgga tttagacaat
    11161 aaacaagaaa acatattaac gtatattcca aaagagatga caatacttcc aaatacatca
    11221 ggagctacta atgcagaaga agctgtaaga atagctagaa tatctagaaa aatgggatgt
    11281 ggagatttta taaaaataga agtaatatca gatactagat atttacttcc tgataatgaa
    11341 gaaactataa aagctacaaa gatacttgct gatgaggggt ttatagttct tccatatatg
    11401 acaccagacc tttatgctgg aagaagactt atagaagcta atgcagcggc agttatgcca
    11461 cttggagctc caattggttc aaatagaggt cttcaaatga aagaaatgat aagaataatg
    11521 atagatgagt tggatatacc aataatagta gatgcaggta ttggaaaacc atctcaagct
    11581 atggaggcta tggaaatggg agctgatgca gtacttgtca acactgctat agcttcagca
    11641 ggagaccctg tacaaatggc tagagcattt aaattagctg tagaaggtgg aagagaagca
    11701 tatattgcaa aaacaggaaa tgtgtcagaa tttgcaaatg catcttctcc tttaacaggt
    11761 tttttaggca atttatagaa agtggtgatt ggtgatgagt ttttatgatg taatagaaaa
    11821 atatagggat tttgattttg atggatattt aaataatgtt actgataatg atgttttaag
    11881 aagtttatca aaagacaagc ttgaagattt tgatatatta aatttgcttt ctaaaactgc
    11941 agtaaaacat ttagaagata tggcacaaaa agcacataaa ttaagtgttc aatactttgg
    12001 taaaacagta tgtttatata caccaatgta tatagcaaat tattgtgtta atcaatgtgt
    12061 ttattgtagc tataatataa agagtggaat aaaaagaaaa aaattaacta tggatgaaat
    12121 tagagaagag ggagaagtaa tttcaaaaga aggtttcaaa catctacttg ttttgactgg
    12181 agaaagtagt tttcactcaa gtgtagaata tataggtgag gctatagaaa tattaagaga
    12241 gaaatttccc tctataggta tagaagttta tccaatggaa gtagaagaat ataagtacat
    12301 tgtagataaa ggtgttgaag gattaacagt atatcaagaa acttatgacg aggaaatata
    12361 taagagagta catataaaag gcccaaaatc aaattataaa tttagactag atgctcctga
    12421 aagaggggca aaagcaggta tgaggacttt atcaataggt gcacttttag ggctaaatga
    12481 ttttagaaaa gaaacttttt ttacaatatt gcatgggaaa tacttaaaaa cgaaatatcc
    12541 tcatattgag ctttcatatt caacaccaag aatgagacct tttaaagggt gttttgagga
    12601 attggtagat ataagtgata ctgatttagt tcaagctatg gtatgtatga gattatttga
    12661 ccctcatgca gctataaata tatccactag agaaaattta gaaatgagaa gtcatataat
    12721 acctttagga gtaactaaat tgagtgcagg tgtatctact gatgtaggag gtcattctca
    12781 agatgaacat gatactgctc aatttaagat aaatgatgaa agtactgtta aagatgtgga
    12841 gaaaatgcta aactcaattg gttatcagca tgtgtttaaa gattgggaaa gattttaaat
    12901 gtatctgata acaaacagaa aattatgtag tgaggaacga tatttagaag ttattaaaga
    12961 gtctatattg agtggtgtag aaaatataat aataagagaa aaggatttag aatatcaaga
    13021 gctaagaaaa ttatatatga aaataaaaac taaaatcaat tgtatagatt ttcaagagca
    13081 aatatcagat gaaagtttga aaactaatat aaatcaaaaa gagtttagaa ataaattcaa
    13141 ggttaatttt ataataaata gtaacattga attttttgaa aaagtggatt gtcaaggtat
    13201 ccatcttcct tttaaattat tcttgaattt aattgaaaat aagtataatt ttaatgaaaa
    13261 taaaatacta ggattatctt tacataaagt agaggaagta gattatttag aaaaattaat
    13321 aagaaatcaa aatataaaaa tagattacat aacattatca catatatatg aaactaaatg
    13381 taaagaagga ttaaatccaa aaggaattga gcttttaaaa gaagctaaaa agattactga
    13441 tattaaaata attgcacttg ggggaatttt accatctaat gtcaaggaaa cattaaaata
    13501 ctgtgatgat tttgctataa tgtcaactat aatgaggtct aaagatatta aaaaaaccat
    13561 atcaaattat aatgaaaagt tgaattagta aatatagtat attgaatttt taacttaaat
    13621 aaaaataaaa aagttatctt ttaaatttta aaaggtagct tttttatttt atatctttgc
    13681 taataaaaaa ttgtaaaaag ggaaaacttt agtcagagag attaaacaat atcctagaaa
    13741 gatttaaaat ttagtaaagt atggaggaca ttataatgac tgacagtaaa aagaaacgat
    13801 ttattatacc acacactttt acaataatat ttttactaat agtcttgatg gcaatattga
    13861 cttggatagt accaagtggt gaatttagta gagaagatgt agaaggtaga atggtagttg
    13921 taccagggac ttatcaaagt gtagaaagta atcctcaagg atttgcagat gtttttaaag
    13981 caccaataga aggctttatc gattcagctg aagttgtagg ttttgtttta atagttggag
    14041 gtgcttttgg tattgtaaat aggactggag ctatagaagc aggaatagct tcggttatct
    14101 ctaagtttaa gggaaaagaa cttcttataa ttccaatatc aatgatttta tttggacttg
    14161 gaggtacaac atttgggatg gcagaagaga cattaccatt ttatatgata tttgttccac
    14221 tcatgacatc cttgggatat gattctctaa cagcaatagc aattgtattt ataggagcag
    14281 cagcaggagc agccgcttca acaataaatc cattttcagt tggtattgca caggcactag
    14341 ctcaacttgc tcctggttca ggagtaactt acagagtagt tatttttata gcatatatac
    14401 taataagtat agcatttgtt atgtactatg ctaagaaggt aaaggaaaat cctgaaagtt
    14461 caatagtata tgaaatagat aaaaataata aatcaatact taatcaaact tcaacagaaa
    14521 taaaagaatt tacttttaaa gaagtatctg ttcttgctat atttggaata gggatggcaa
    14581 taatgatttg gggtgtttta agtcaaggat ggtacattcc agaaatatca atgtgttttg
    14641 ctgcaatagg tgtgctttct ggattggttg gtagattatc tcaaattgaa atctcagaaa
    14701 gttttataga tggagcaaaa gatttagcat ctgcagctct tgctatagct ttggctcgtg
    14761 gaatcgtaat aattgctcaa aatggattta taatagatac tatattaaat tccgcagcag
    14821 cctttctagg tgggcttcca aaggttatat ttgtatattt atcatttttc ttggaagctg
    14881 cattggcatt tttaattcca tcatcttcag gtcttgcttc acttacagta cctgtacttg
    14941 ctcctcttgg tgatttggtt aatatatcgg ctcaagtgat agtaaccgct tatcaatttg
    15001 gtgtgggtct tacaaacttt ataactccaa cttcaggagt actgatggga gcacttgcag
    15061 tagcccatat tccattttca aaatgggtaa gatttataat gcctatcttg ttgattttga
    15121 cagctgtatc tctagtcttt ttaacaatag gtatttatat agggttttaa ttaagtgtag
    15181 ttttatttaa gatatttagt aatggtataa aatgataata tgttttcaat aaattgccac
    15241 taatttaatt agtggtaatt tgttttttgt atttaaaaat aaatgtattt acttgtattt
    15301 tgaattaata tctacttaga cattgtttta attgttatat ttttatttaa ttaagcattg
    15361 atttatcatt ttttatattt catataacaa tgcaaaaaat aatttttata tagtaaatga
    15421 caaaagttta caataaatgt atattttctt actcattcct taaaattact catttagttt
    15481 aataactctc atttcatatg taaagttggt aaaaaaatta aaattaaaag taaaaaacta
    15541 aataaattaa tgttaatttg aaatatatat gaaggttttt ggagaatact tacttatgat
    15601 aggaaaaaat gttaaaataa tagtgtaatc aaatataaag gactgaaata attaacagtt
    15661 attaaatatt aggagtgtaa aaatttatgg gattaaaaga atttgatttt attgaagagt
    15721 ctattgatat gttgaggaca atgtcaccaa cattagaaac tatatcagat gaaatagaag
    15781 aatattttga aaatatcttg gatgaaaaga atcaagaata tataaatgta acctctagaa
    15841 taaaatcaga atcaagttta agagaaaaga taattagaaa tagatattta aaaaaatatg
    15901 gagaagcaag taatcttatt cataatgttt ctgacttaat aggcctaaga attgagtgta
    15961 gatttattga ggatgaaaac aaaatatata gacttttgag aagatatttt aataaaacag
    16021 atgacaaaat aaactattat aataaagaga ataagaatat taaattaaaa ctatctgaga
    16081 ggcaacccaa taagcaaaaa aatggatttg aaatatataa aatagatgga gtttttgcgt
    16141 atttagatag agaagtaaaa tttgaacttc aaattaaatc tcttgtaaat gtgttttgga
    16201 gtgaaataga acataaaata atttataaaa acaatacata tttacttgct gataaattta
    16261 taaaggatat gatggactct ataaagaata atcttactat gatagataat cagcttttaa
    16321 gtatttataa aaactttcat tcagggaagt cttttaatat gaaagtaagt aaaaaagaaa
    16381 ttgaaaaatt atttgcgaaa ctagtctatg acgctttttc tgaaaagatg aataaaagta
    16441 taggttttgt tgtagatttt aaaaaaccat gtgagactat attgaactat tcttttaata
    16501 aacaagatat aagtgatgaa gttttaggta attttatgct tgatgagttt gcaagattaa
    16561 atgaaattgt aaacaaggat atagatttta atgagcaaat agaatttgaa agagaaccaa
    16621 catttgatga taagttttgt aaagacctag gaaatcattt tagaagtaga ttaaacacag
    16681 aatttccatg gaacctattt tttagaatat tatttgaaat agagccatat aacaatacca
    16741 aagactttga aaactttgta gagtttataa aagaaaatgt gctactagaa gaaaataggg
    16801 agcaattatt atgccaattt gaagaggata gtaagcttat aattgaagat atatatgatt
    16861 gtatatttaa tagtataagt gagattgata gtgttgaaat tttatataac tataatcttg
    16921 aaaaaataaa ctttacatca agtgaaatca taaattgtat atgtagagaa tatgagtgtt
    16981 atggtgaata cttagaagaa aaagaaaagc ttatgaatac atttaaagaa aagattattc
    17041 aaatctttga gtaataggtc aatgctgaag tggcgctaat gcgccatttt tcaaatatat
    17101 atgaattaag taaaattatt ttggaggagg cgatttgtat agagaaaaca ggtgtaattt
    17161 tggcaggggg aaaaaattct agaatgggta gagataaagc ctttttagag ttacatgata
    17221 agctttttat tgaaattgca atagaagcat ttaaaaactt tgatgagttg ataataatat
    17281 ctaacaatga agaattatat tctaaatacg atattaaagt ttataatgat atagtaaaag
    17341 atgttggtcc aattggagga atatatactg ctcttgaata tgcaaagtat gatatagtta
    17401 caattgcatg tgatatgcca tatctaaata atcaaatagt cgaaagaata gctaataaaa
    17461 tggatgataa aagtgtgatt tcagtgacaa atggaaagtt acaaccattg tgttctggat
    17521 ataaaaagag tataataaat aaagtatctt tatgtataag ggaaaatgat ttaaagctca
    17581 gaagctttat agataaaata gataaaagtt atatatactt tgatgaagaa gatttatttt
    17641 taaatgtaaa cactgttgat gagtataaaa aattaactaa agtttaatta gctatggttg
    17701 taaaatagag taggatttat atggaggaga gtttatgaat ttacttaaat gcgattcttg
    17761 ggcagtaata ttaaataata gcgataaaga aagtaaagca tataaaatat tagatgaact
    17821 taaaaggaat atgtacaaag ttgttgctat agatgaagag aaaaaaccta ttgaaggaat
    17881 agatgtgtat gaatgtttaa aagatgttcc acacaatatt gatgttgtag ctattattga
    17941 taaacaatct aagatggatg tgattttaga agaagtagag cttttggata tacaaaatat
    18001 gtggtttgaa aaaggaagct ttagtgaaat gatgattaaa aagactaaag atttaaaatt
    18061 aaatatagaa tataatttaa gtttatatga tgagttgact agataaaatt atatttctta
    18121 ataataaaaa tataattagt taataaaaca tattatttta tagtatagat tggttaataa
    18181 aagtacagat tggttaatga aacattaaat tacaatattt gttaaaaaaa ttgtgagtta
    18241 catgatataa tgtattaaat aggatactaa ggagttgata tattatgtta atagtaacta
    18301 cagaaaaagt agaaggtaaa aagatatcaa aggttttagg attagtgaga ggaagtacaa
    18361 taagagcaaa acatgttgga aaagatatag gagcaagttt taagaatctt gttggaggag
    18421 aacttactgg atataatgaa atgctcactg aagcaagaca aattgccata ggtagaatgg
    18481 ttgaagatgc agaagctaaa ggtgcaaacg cagtaatagc atttagactg tcttcagctt
    18541 cagttatgca aggagcagca gaaatgcttg cttatggaac agcagttgtt ttagaagatg
    18601 ataatagtat tcttgaaaaa taaaatataa atagttaaaa agaggcatct tttaaaaatt
    18661 ttatggatgc ctttttttat ttgaaaaaac taatttgaga agaaatttta actattatga
    18721 caataaaatt agggtgatat tatataaata agtaaaatta tattatataa tgtatggtat
    18781 agactttaaa aattttattt gtagagaaaa atatggacaa tgttataata attatagaaa
    18841 ttaaaagtac ttacattcat tatgtatgtg acatgtataa ttagttttat atttaataca
    18901 gttttgaagt aatatcttca ttttgagaac gttggaataa taaaaaatgt attattatag
    18961 gtgatataaa taaaaaataa atataatatg ttatattgct gatagaaaag agaataaaag
    19021 aaaataggtt ttattaaaag agatttttat ttataggact taatcaatgt atagaggggt
    19081 gagaacatat gtttaatgtt gcaattataa cacttagtga taaaggatat gaaggaaaaa
    19141 gagaagatat aacaggaaaa aagttaactg aatttgttga aaatacagga gtttataaag
    19201 ttactgaata tgtacttata aaagatgata aggaaatgct aaaggaaaat attataagac
    19261 tttgtaatag taataaaata gatttaattc ttacaaatgg aggtactggt ttttcaaaaa
    19321 gagatataac cccagaagca actaaggaag tgttggaaaa agaaatacca ggtctgagcg
    19381 aatatatgag aatgaaatcc acagaaatta ctaaaaaggc catactaagt aggggtgtga
    19441 gtggaataag aaataattct ataataataa atttacctgg aagtcctaaa ggtgcagttg
    19501 aaaatctaag ttttataatt gatgttttag accatggcat agaagtttta agaggagaag
    19561 ctacagaatg tgccactaaa aaaagtgaat agtatataat ctagatagat aaaaaataga
    19621 caaagaggta gataaagtat gattgataag tatggaagaa aaatagacta ccttcgtatt
    19681 tcacttacag ataagtgtaa tttaaggtgt gtctattgta tgccaccaga tgtaaaattt
    19741 gataaaaatt atataaatga aaatctaagc tttgaagact acaagtttat tataaaggct
    19801 atggctgaac aaggtataac aaaaattaga tttactggag gagaacctct tttatatcct
    19861 aaattaaatg agcttataaa atttacaaaa gaagaatgtg gaataaataa cataggaatg
    19921 accactaatg ctattggtct ttctgataga atttttgagc ttgaaaaatg tggcttaaat
    19981 aaagttaata taagtttaga ttctttgaag gaatataagt ataaatccat aacaagagga
    20041 ggaaacttaa aagatgtctt aaaatcgatt gaatcttgtt taaatttagg tataaaagta
    20101 aagttaaatt gcgttgctat aagtgggttt aatgatgatg agcttaaaga ttttatgttt
    20161 ttaacagtta aatcaccaat tgatgtaaga tttatagagc ttatgccatt aggtgaggct
    20221 aatagagttt ataagaaggg ttattttaat gtaaaagaaa tgattgaaaa aatatatggt
    20281 ttatataaag tcaaaaatga tgaaaaaagt acagcagaat attatatgtt taaaggagct
    20341 aaaggaagaa taggaattat aactccatta agctgttcat tttgtaacac ttgtaatcgt
    20401 attagactta cttctagtgg tgcaataaaa ttatgtctac attcaaaaga agaaatagat
    20461 ataaaaccat ttttacataa accattgtta tttagagaat ctatgaaaga tataataaaa
    20521 caaaaaccag aaaaacatca tttaatagaa aataaatgta gtgatacaaa taggcgtatg
    20581 tatgaaattg gaggataagt aatgttaaca catataaatg aaaaagggta tgccaagatg
    20641 gtggacataa gtgaaaaaga tgatactaaa agaacggccg tagctacagc tacaattact
    20701 cttagtaagg aagcattaga aaaagtaaaa aatggtcaga taaaaaaagg agacgtattg
    20761 tctgtagctc aagttgcagg tataatggga gctaaaaata catcttcaaa cataccaatg
    20821 tgccatcaga ttaaattagt tggatgtgat ttagaatttg atgtagatga ttctatgtct
    20881 gctattaaga tatacgcaac ctgtaaatct cttgggaaaa ctggagtaga aatggaagct
    20941 ttaaatgcat gttctttagc agcacttaca atttatgata tgtgtaaagc agttgataaa
    21001 aaaatggtta taagtaatat ccatcttgtg gagaagactg gtggaaaatc aggagatttt
    21061 aagttcgagt aaattaataa tatattgggt taaaatttgg aggaaatatg gcgaaggtag
    21121 tgtcaataaa tataagtgag aaaaagggtg taattaaagt tcctgtacaa tgtgcagagc
    21181 taaaaataaa tcatgggata gttggagatg cacatgcagg aaactggcat cgtcagataa
    21241 gcttacttgg aaaagaaagc atagataaaa tgaaaatgaa aggctttgac caattaaaat
    21301 atggagattt tgctgaaaat gtaactacag aagggataga agttttttcc ctgcctgttg
    21361 gtacaaagtt aagaataggg caatgtgaag ttgaagtaac tcaaataggg aaaaaatgtc
    21421 acaatggatg tgaaattaag aaattgacag gtgactgtgt gatgccacga gaaggaattt
    21481 ttgtaaaggt tctaaaagaa ggaattataa atgtgaatga ttcaatagat gtaatctaat
    21541 gaagtcacta ttaaaatata attataagta aatactatta agatttaaat atatatttta
    21601 agagagaatt aattttaata aaatatttta gttataaatt ggaatat
//
LOCUS       DAYAPA010000065        21553 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.065, whole genome shotgun sequence.
ACCESSION   DAYAPA010000065 DAYAPA010000000
VERSION     DAYAPA010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21553
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.065"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(16..894)
                     /locus_tag="ACGI59_002224"
     CDS             complement(16..894)
                     /locus_tag="ACGI59_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFG9740010.1"
                     /translation="MRYDIAIIGSGPAGLSAAINAKIRNKTIIMFGNDNLSNKLVKAP
                     SIDNYLGFYDISGDELKDKFKSHIDSMDISIENKRINNIYAMGEYFTIMSGNDMYEAT
                     TVILATGVEYTRPIKGEEEFLGRGVGYCATCDAPLYRNKKVAVIGYNEESKEEANFLS
                     ELTSKTYFIPMYKKDNLMRSSDNLDDSIEVIHDRPVQIDGDKLVNKVSFKENHIEVDG
                     VFVIKDSTAPSALVPGIEIDGIHIKVDNNMKTSIDGCFAAGDCVGKPYSYIKAAGQGQ
                     IAALNAVYYLDKLKRA"
     gene            complement(911..1228)
                     /gene="trxA"
                     /locus_tag="ACGI59_002225"
     CDS             complement(911..1228)
                     /gene="trxA"
                     /locus_tag="ACGI59_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HFG9740011.1"
                     /translation="MAKVINTSEFRSSVEGSKGVVVVDFFATWCGPCNMLGPVFAELG
                     EEMKDKARFVKVDIDESLEIAQQFNVSTVPTMIIFKDGKPVETLIGFMPKNKIEMQVK
                     SYL"
     gene            complement(1378..2610)
                     /locus_tag="ACGI59_002226"
     CDS             complement(1378..2610)
                     /locus_tag="ACGI59_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFG9740012.1"
                     /translation="MINKNVFTSTKNHLIKMYIIVVGSFLIIFSIFIYSYFRGLTYSG
                     IDSEINDELEYIVSQFKRTSFLNPIRLKDPKDMVYVYEDGRISYYTQNEYFDELLPDR
                     RLDKKNSFFKYTENGYTFRELNVDVGRYQIQIIRNIDSEMNSLRQLTSVLIIGILISV
                     IITYFVAVYLTRKALIPIETAWKNQAKFIQDASHELRTPITIVSSKLESMLKSPESTV
                     NDEVETIATAMKETRRLKKMITDLLSLTKEDSIVKVNLEEIDLEKLLEEISEDYIDIA
                     EFQEKRFVFNSKLKNKVIITDKNKLRQLILIFIDNAFKYTKLGDEIALELKEDIEDEV
                     TLLISDTGIGIKKEEIPLIFDRFFRSENVRNKDLEGSGIGLSIARMISLNLSIDINVT
                     SDVDIGTTFELSIPKKLK"
     gene            complement(2600..3271)
                     /locus_tag="ACGI59_002227"
     CDS             complement(2600..3271)
                     /locus_tag="ACGI59_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFG9740013.1"
                     /translation="MKVLIVEDNKILLESVVEELSKHFETEKCEDGEEALYLINQNIY
                     DLVILDLMLPNINGFDILKKMRINNIDTPVLILTAKETLDDKVEAFTIGANDYLTKPF
                     YMEELVARVYAILRTNGKIKERNGLEFKSLYLDTLEKRVYIEKEEIKLQNKQFNLLEY
                     FVLNKGSILLKEQIYDRIWGIDSDATIEIVEVYVSNLRKKLSKYGYDKYIKTKRKVGY
                     IFDDK"
     gene            complement(3287..4123)
                     /locus_tag="ACGI59_002228"
     CDS             complement(3287..4123)
                     /locus_tag="ACGI59_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9740014.1"
                     /translation="MNKIVKKSISLMIILTIFIFMLTACEKDEQPDSVDIQTEDKNEI
                     KIDEGNAKVLNIGKSEIINIDEGDKVDTSTKVENNSTFNISNVELIYNEYDENKKIIS
                     SDSKALLDMTLMPGKVAYIECGHKTFVKGVEVYAYEYEAEGKIVYVNLKENTINIRNN
                     NIKLENSSQYEVLSTSELKKVNESNEGITYQVKVKNSSSKDLGNIILKTAEVNENGEY
                     LTVNRVPSYKILKPSEETDIDILCSTKAKSVEIVGYTYDDIKEKANVDIDLKSHKVKI
                     DK"
     gene            complement(4149..4823)
                     /locus_tag="ACGI59_002229"
     CDS             complement(4149..4823)
                     /locus_tag="ACGI59_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFG9740015.1"
                     /translation="MNNNEDRIDKFLELQSKGFKFAEIAQNLKISQSTLRTFLNKKGY
                     KSEKGKYILKDELNYNKSLDNRNLEEEKAKEYEKSKKNTISNRKKVSTKKKISNEKAL
                     SKSVEQIEFNDTGLKNAKPKQNIKPKKDRKINITQEDLDKLCEVYDWYLEVKDYNIKP
                     KKVSNKKDVHIDDEHIDELKTTRIRIDKEIWNDFERLCSNSNFEKHQILTQALKEFMK
                     KYKNLL"
     gene            complement(4837..5565)
                     /locus_tag="ACGI59_002230"
     CDS             complement(4837..5565)
                     /locus_tag="ACGI59_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HFG9740016.1"
                     /translation="MMFDRGKWNKLDYNELLEYMMSIQDIKYRDFNKKLIPGTENIIG
                     IRVPNLRKLSKEISQGNWKEFLEVAQDTYYEEVRLQGMVIGNINSNFEETLYYVKRFI
                     PKIDNWSVCDGFCSDLKSMKKYKENMYDILKKYVYSKNPWEIRFALVMFLIYYVDDKH
                     IKEIFEYCNNIQSEEYYVKMGMAWLLSICFIKCEEETFLYIKNNNLDDFTYNKMLQKI
                     IESNRVDLEKKNIIRSMKRKTRRC"
     gene            complement(5692..6381)
                     /locus_tag="ACGI59_002231"
     CDS             complement(5692..6381)
                     /locus_tag="ACGI59_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFG9740017.1"
                     /translation="MDRYSEITNKNQREIVLLKGFPCIWGKCSFCDYIDDNSNLEEEM
                     NKLNLKVLKNVTGKYGVLEVINSGSCFELPKDTLEKIKCIIKEKNIKKLFLESHWSYK
                     NRLKEMREYFEIPVVFKIGVETFDNDFRNNILNKNANFKTPQDVKEYFDSPCIMVGIK
                     GQTKEMIDKDIEIILNTFEKATVNVFINNSSSIKRDEELVKWFVSKYKFLDENPNIEV
                     LYNNTDFGVGD"
     gene            complement(6389..6952)
                     /locus_tag="ACGI59_002232"
     CDS             complement(6389..6952)
                     /locus_tag="ACGI59_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFG9740018.1"
                     /translation="MKRKICVKTITLIAFGISINIIGAFIAMGLRLPVYLDSIGTIMI
                     ASLLGPKYAVVTGVFGSLISGITFDVYSLYFAPVQISTGLLAGIAFKKNFLRGLKTPL
                     GVLLFAIPTSIISSIISAFLFGGMTSSGSSYIVQILKVLGLGDVFSVFVTQVFTDYGD
                     KLLAVVLVNLGLNAVPKTLKVSLTGGK"
     gene            complement(6954..7922)
                     /locus_tag="ACGI59_002233"
     CDS             complement(6954..7922)
                     /locus_tag="ACGI59_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HFG9740019.1"
                     /translation="MEKRKVIIDCDPGIDDSLAILLALNSPELEVIGITTCCGNVPAN
                     IGAENALKTLQMCSSLNIPVYIGEEAPLKRKLVTAQDTHGEDGIGENFYQKVVGAKAK
                     NGAVDFIINTLYNHEKVSIIALAPLTNIAKALIKDKKAFENLDEFVSMGGAFRIHGNC
                     SPVAEFNYWVDPHGADYVYKNLSKKIHMVGLDVTRKIVLTPNIIEFINRLDKKMAKYI
                     TEITRFYIDFHWEQEGIIGCVINDPLAVAYFIDRSICKGFESYVEVVEDGIAMGQSIV
                     DSFNFYKKNPNAIVLNEVDEKKFMYMFLKRLFKGYEDIIDSVEGVI"
     regulatory      complement(8023..8066)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            8432..9061
                     /locus_tag="ACGI59_002234"
     CDS             8432..9061
                     /locus_tag="ACGI59_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFG9740020.1"
                     /translation="MSIKERRKNEKEEMKKKIMDASIEIINQHGYENLSIRKIATKIE
                     YSPTTIYLYYKDKAEIISDMTNKLYNTVESNAIDIMNNCSSLPIDKQIIEIMTSFIKT
                     LSSEPEMAKSIMHSRMNIIFASENTNNTPSNNGIKMLDKFLSIGIEQGIFKKNIDKSS
                     WMLISALLGFVLCVVENKLYSLHNFSQLIENFLDILVGGLYNENSQQGY"
     gene            9036..9440
                     /locus_tag="ACGI59_002235"
     CDS             9036..9440
                     /locus_tag="ACGI59_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HFG9740021.1"
                     /translation="MKIRNKATNKKKKYVKISIALFFVFICIIIVIVSNLKPENLVVK
                     DIDINNVKNGIYTGSADNNLVKATVSVEVNNGKIQNINILKHDHLLGKPAEKITTSII
                     KQQSLDVDAITSATYSSNTIRKAVENALRKGE"
     gene            9443..9919
                     /locus_tag="ACGI59_002236"
     CDS             9443..9919
                     /locus_tag="ACGI59_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HFG9740022.1"
                     /translation="MNTIIIYSSKYGCTKDCANILKNKLSDNVTIVDINNNNNKIELS
                     KFDKIIIGSSIYVGSVSKKIQVLCNDNVELLNKKQVGIFLCCGFSEQADKYLKSNFPS
                     SLLESANAIGIFGSEARLEKMKFLDKLIMKAVSKGNYDSFRISQDNIDNFLINLNS"
     gene            10491..11156
                     /locus_tag="ACGI59_002237"
     CDS             10491..11156
                     /locus_tag="ACGI59_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HFG9740023.1"
                     /translation="MIKLIGILIVVIGFILKIDTLFTVLLAGVATGIVAGLDFNQIFT
                     ILGDSFVSNRGVSLFILTLPVIGVLERYGLRQRAVSLIEKLKRLTTGKVLTIYMIARQ
                     IAGALSIRMSGHPQFVRPLVNPMAQAAGLSNSDELKESDEEAIKALSAASENYGNFYG
                     QNLFAGSSGVLLIASTLTQFGYNVTGLNIVKASTIMAVIALVVATIQFTLYDKKLNKS
                     HKK"
     gene            11178..12110
                     /locus_tag="ACGI59_002238"
     CDS             11178..12110
                     /locus_tag="ACGI59_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HFG9740024.1"
                     /translation="MQNFINISLEIFYALMGFLMIVIAYKSFTTINNNKKYGTSLFWI
                     LISLPFIFGRLIPANIIGIILILSSLLTLSKQVVFAKYEEPDENFGKEQADKLKNKIF
                     IPSLILAFAAVVVAMSLSNFDNSSQFAIGVGSIIALISALIITKAKPATSVQDGSRLL
                     QQMGPASMLPQLLVALGALFTQAGVGEVISTMISGVVPADSRLFGVIAYVLGMVIFTM
                     IMGNAFAAFSVITAGIGIPFVLSQGGNPAIIGALALTAGYCGTLLTPMAANFNIVPAA
                     LLECKNDYIVIKYQAPVALVLIIAHILVMYFLGF"
     gene            12131..12772
                     /gene="pcp"
                     /locus_tag="ACGI59_002239"
     CDS             12131..12772
                     /gene="pcp"
                     /locus_tag="ACGI59_002239"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016920 - pyroglutamyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HFG9740025.1"
                     /translation="MKILLTGFDPFGGEPINPAQEAVERVNNNINGAEIIKITIPTVM
                     TKSVEAIDKAIQEHNPDIVISVGQAGGRFDITPERVAINIDDFRIKDNEGNQVIDTII
                     KEDGEPAYFSKLPVKAMVKHMNENKIPASVSNTAGTFVCNHVMYGILYMIDKKYPNIR
                     GGFIHIPYTTSQVIDKKNTPFMSLEEIVKGLELAIEACIIYKEDVKEIGGEIS"
     gene            complement(12892..13224)
                     /locus_tag="ACGI59_002240"
     CDS             complement(12892..13224)
                     /locus_tag="ACGI59_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFG9740026.1"
                     /translation="MSIDCISNTDLSETGFSYTLSLISGKYKMIILYCLVEFEVVRYN
                     ALKRYINTISYKTLSLSLKELEADNLIIRTEYPQIPPKVEYSLSERGKSLIPILDAMC
                     EWGERNRP"
     gene            13490..14110
                     /locus_tag="ACGI59_002241"
     CDS             13490..14110
                     /locus_tag="ACGI59_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HFG9740027.1"
                     /translation="MKVLLINGSPNQYGCTYTALNEITKVLSKHDIKTEILYLGKETI
                     PGCISCASCFETGKCIRNDKVNELIEDLDNIDGIIIGSPVYFSSATGQLTSFLDRLFF
                     IAGSRMATKLGASVVSCRRGGASATFDQLNKYFSISNMPIVSSQYWNQVHGFTPEDVM
                     KDEEGLQTMRTLGENMAWLLKCISAGKKAGIKEPQYEETIMTNFIK"
     gene            complement(14435..15259)
                     /locus_tag="ACGI59_002242"
     CDS             complement(14435..15259)
                     /locus_tag="ACGI59_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraX family protein"
                     /protein_id="HFG9740028.1"
                     /translation="MDFFKKGISGFTIKILALIFMTFDHIAAFMPQTMQIPIWFHWVG
                     RISAPLFIFMAVEGFYHTSNRKKYISRLYIWSVIMAIGNQIINNVFSHPEGAIIINNT
                     FSTLFLIAIYLQAIEFIKKFRKEKEIKYFIIGLLMIIIPIILGIFTVALLFKVTNRVI
                     ALFMILVPVPFLVEGGPIWIILGIIFYLCRGKKFSLSICYVLMCIFIFTTMSNGDYSL
                     KNSILQNYQWMMIASLPLMLLYNGEKGKSMKYLFYLYYPIHVYILYILGIYLINGF"
     gene            complement(15270..16673)
                     /locus_tag="ACGI59_002243"
     CDS             complement(15270..16673)
                     /locus_tag="ACGI59_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFG9740029.1"
                     /translation="MKWKITRNFIFTIVFVAISVVIINIISILYVISTNSFFKVVDSG
                     NNPEEFARSFEKDLYEKDGEFKLSKIGAEKLEKSNSWIQVLNDLGEEVYGVNVPKDTP
                     KKYTPFQMVNNYKYIETKYVNFVLEKYLNKKHLNIIVGIPSRDISRIILTYSQNNIKK
                     TLNKVIIITLVIDSVVALGVGYLFSRKLTKPISSVLWSIETMANGNYSLYLKDRGIYE
                     EVFKNINMLADTLRVNEVERKENEELREEWLANITHDIKTPLASIQGYAEIINDKDYE
                     FEEDEIQEYTEIIYNKSKYIKDLVDDLNLSTRLKNNTIVLDKKKINLVSLVRDIIIDI
                     LNDNRYKNRNIEFESNEDLIEVYVDSILFRRAITNLIFNSIVHNSEGTLISVEIVKKD
                     NIEIIIKDDGIGISKSDLKHIFKKYYRGTNTGEMHKGSGLGMAISKEIIEIHKGKIYV
                     SSEIGIGTKIIIEIKQN"
     gene            complement(16677..17402)
                     /locus_tag="ACGI59_002244"
     CDS             complement(16677..17402)
                     /locus_tag="ACGI59_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFG9740030.1"
                     /translation="MENRVLIIDDEVEILKLLETVLKKEGLNNIYTAKTKKEGLELFK
                     SINPDLIVLDIMLPDGEGYDICKEIRKTSNSPIIFLSAKTEELDKLLGLAIGGDDYVT
                     KPFSPKEVAFRVKAHLRRLSYFSDAQNESKNLNNNEEKIISFGPYILNESRAELIKDG
                     KSIGLSAKELKILSLFAHNQNQIISKEKLWDKVWGEDYVGFDNTIMVHIRKIREKLED
                     NPSKPEYILTIKGLGYKLAVKED"
     gene            complement(17514..18287)
                     /locus_tag="ACGI59_002245"
     CDS             complement(17514..18287)
                     /locus_tag="ACGI59_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9740031.1"
                     /translation="MMYLVKAEMIKILKSKSVWIIWLFLIFFGFFLIRKFDVLDTYAD
                     IFYKIEGSIPLIGLIMFIITSGNYIKEYDSNMTGLINTTKKGKKSVVLSKLVANGLIL
                     SIINISFILLVGIRGFSFFDFKNLNESIHNLWYFGNSNLNLTVIQMYIIVILTTIFAS
                     FIFAQIGLTLSSIFKSAVIPFILGGLIMAIPYFSVGFIPDKAIKFMSVTPNWIMMSQQ
                     MVKYNVPSILIVFSIVISIILMIVLTKITYENFTSSKRF"
     gene            complement(18293..19180)
                     /locus_tag="ACGI59_002246"
     CDS             complement(18293..19180)
                     /locus_tag="ACGI59_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9740032.1"
                     /translation="MNKLEIENLSKIYGKKVANDKITVTLENGVYGLLGPNGAGKSTL
                     MKQITTLIKPDEGQILYNGEDIFKMDADYRNILGYLPQEFGVYKNFTAKEFLQYIGAL
                     KGLRGKYLNSKIGELLDLVGLYDVRNKSIGKFSGGMKRRVGIAQVLLNDPKIIVLDEP
                     TAGLDPQERARFRNLIAKMSRDKIIILSTHIISDIESVAKETIMIKDGKLLMKGTHKD
                     ILSHMENKVYNVQVKNECEVDDIQSKYKVVSIQSDIDSVVLRIVSEDIPSEDNIQPVQ
                     AKFEDVYMFYFDLEDAREV"
     gene            complement(19194..20216)
                     /locus_tag="ACGI59_002247"
     CDS             complement(19194..20216)
                     /locus_tag="ACGI59_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9740033.1"
                     /translation="MVNWEIKKLAKSKSMFISLGILVLILMISIFITPVLETESYYID
                     KEKGRIEDTRSGIDVGNEKFQNKINVLKQFSNQTDTGEFSKKIELMSKKKLDNLSVNE
                     YKDISFWKVFNYRVTNSLINVGMLIIISIIISNIYVDEVVTSVKDIILSSKEKKKALK
                     SKIFVALLVPLVIYSVYLMGIFIITCMQQGVPVNGDLESFRIVDNVVALKTNLSITKY
                     VILNIGIELLMFEGWAMIAIFGSFISKSSISSISFFIFIISITKIMSVIHILPKKLLS
                     VVSNVNYYDLIFGFNKIIGNYLGDIMIFKVNIDIVNIAIGLLMAILIGAMSLCFLVIK
                     SDYISR"
     gene            20791..20967
                     /locus_tag="ACGI59_002248"
     CDS             20791..20967
                     /locus_tag="ACGI59_002248"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9740034.1"
                     /translation="MFIGEIDKCTHILTAYISSSYDYCNFLDTQLDDFISEYGETVVE
                     TCLHQVLLLVSRYN"
BASE COUNT         7487 a         3382 c         2313 g         8371 t
ORIGIN      
        1 taatcaatat aaatattaag ccctctttaa tttatctaaa tagtaaacag catttaaagc
       61 tgctatttgc ccttgaccag ctgcttttat ataagaatag ggttttccaa cacaatctcc
      121 agcagcaaag catccatcta tactagtctt catattattg tcaactttaa tatgaatgcc
      181 atcaatctct attccaggaa ccaatgcact tggagccgta ctatctttta taacgaatac
      241 tccatcaact tcaatgtggt tttctttaaa agatactttg tttacaagtt tatctccatc
      301 tatctgtact ggtctgtcat gtataacttc aatagaatca tctaggttat ctgaacttct
      361 cataagatta tcttttttgt acataggtat aaaataagtc tttgaagtga gttcacttaa
      421 aaaatttgct tcttctttag attcttcatt atatcctata actgctacct ttttatttct
      481 ataaagtggt gcatcacatg tagcacagta gcccacacct cttcctagaa attcttcttc
      541 acccttgatt ggtctagtat actctacccc agttgcaagg attacagtag tagcttcata
      601 catatcattt ccagacatta ttgtaaagta ttctcccata gcataaatat tatttattct
      661 tttattttca attgaaatat ccatactatc tatatgtgat ttaaacttgt cttttaattc
      721 atcaccactt atatcataaa aacctaaata attatctatt gaaggagctt taacaagctt
      781 attacttaaa ttatcattac caaacatgat aattgtttta tttcttattt ttgcatttat
      841 agcagcagac aatccagctg gaccactgcc gattatagct atatcatatc tcataatact
      901 cacccatttt ttataaatat gattttactt gcatttcaat tttgttcttt ggcatgaatc
      961 caattaaagt ttctactggt tttccatctt taaatataat catagttggt actgtagata
     1021 cattgaattg ttgagctatt tctaaactct catctatatc aacttttaca aatctagctt
     1081 tatccttcat ctcttcacct aattctgcaa atacaggtcc caacatgtta caaggtccac
     1141 accaagttgc aaagaaatca actacaacta ctcccttact accttcaaca ctacttctaa
     1201 attcagatgt atttataact tttgccattt ttaaatcctc ctattaaact ttattatccc
     1261 tatataatta tactatgaaa tttataaata taaatttact tttataaggt ctttttatat
     1321 tttatactaa ttagtatacc cattcttcat ttctaaaaac aattttttat atttttatta
     1381 ttttaacttt tttggtattg acaattcaaa tgtagttcca atatctacat cagaagttac
     1441 atttatatca atagataagt ttaaactaat catgcgagct attgaaagtc ctattccaga
     1501 accttctaaa tctttatttc ttacattttc acttctaaaa aatctatcaa atataagagg
     1561 tatctcttct ttttttatgc ctataccagt atcacttata agtaaagtta cctcatcctc
     1621 tatatcttct ttcaattcta atgctatctc atcacccaat tttgtatact taaaggcatt
     1681 atcaatgaaa attaatataa gttgtcttaa tttattttta tctgttatta taactttatt
     1741 cttcagttta gaattaaaca cgaatctttt ttcctgaaac tcagcaatgt caatatagtc
     1801 ttctgatatt tcttccaata acttttctaa atctatttct tctaaattaa ctttaacaat
     1861 agagtcctct ttagtaagag acaataaatc agttatcatt tttttaagtc ttctagtttc
     1921 cttcattgca gtagctattg tttcaacttc atcatttact gtactttctg gggattttaa
     1981 catactttct aattttgatg aaactattgt tattggtgtt cttaattcat gagaagcatc
     2041 ttgtataaac ttagcttgat ttttccatgc tgtttctatt ggaattaaag cttttctagt
     2101 gagatataca gccacaaagt aagtaattat tacagaaatt aatattccaa ttataagaac
     2161 agatgtaagc tgtcttaaag aattcatctc agaatctata tttctaatta tctgtatttg
     2221 gtatctacct acatctacat ttaattctct aaaagtatat ccattttctg tgtatttaaa
     2281 aaacgaattt tttttatcta gtcttctatc tggaagtaac tcatcaaaat attcattttg
     2341 agtatagtaa cttatccttc catcttcata tacatacacc atatctttag gatctttgag
     2401 tcttattggg ttcaaaaatg aagttctttt aaattgagag actatatatt caagttcatc
     2461 atttatctca ctatcaattc cactatatgt aagtccccta aaatatgagt atataaatat
     2521 agaaaagatt attaaaaaac tacctactac aataatatac atttttatta aatgattttt
     2581 agtactggta aatacattct tatttatcat cgaatatata tcctacctta cgtttagttt
     2641 taatatattt atcatatcca tatttactta gcttttttct tagattactt acatatactt
     2701 ccactatttc aatagttgca tctgaatcaa ttccccatat tctatcataa atctgctctt
     2761 ttaataaaat tgaaccttta tttaatacaa aatactcaag caagttaaac tgtttgtttt
     2821 gaagttttat ttcttctttt tcaatataca ctcttttttc tagtgtatct aaatataaag
     2881 atttaaattc taatccgttt ctttctttta tctttccatt agtccttaat atagcgtata
     2941 ccctagctac taactcttcc atataaaaag gttttgtcaa ataatcatta gctcctatag
     3001 taaatgcttc tactttgtca tctaatgttt cttttgctgt caatataagt acaggtgtat
     3061 ctatattatt aattctcatt ttctttagga tatcaaatcc atttatatta ggaagcatta
     3121 agtctaaaat gactaaatca tatatatttt gatttattaa gtataaagct tcttctccat
     3181 cttcacactt ttcagtttca aaatgcttgc taagttcttc tactacactt tctaaaagta
     3241 tcttattatc ttcaactatt aaaaccttca taagtatccc ctacctttat ttgtctatct
     3301 taactttatg agattttaag tcaatatcca cattggcttt ttcttttata tcatcatacg
     3361 tatatcccac tatttcaaca ctttttgcct ttgttgaaca caatatatct atatctgtct
     3421 cttctgaagg ctttaaaatc ttatatgatg gtacacgatt tactgttaaa tattctccat
     3481 tttcattaac ttctgctgtc ttaagaataa tatttcctaa atcttttgat gatgaatttt
     3541 ttacttttac ttgatacgta attccctcat ttgattcatt tactttttta agttctgatg
     3601 tagatagcac ctcatactga ctactatttt ctagcttaat attattattt cttatattaa
     3661 tagtattttc ttttaaatta acatacacaa tcttaccctc tgcctcatat tcatatgcat
     3721 aaacttctac accctttaca aaagttttat gcccacattc tatgtaagca actttcccag
     3781 gcattaaagt catgtccaac aaagccttac tatccgaaga tataattttt ttattctcat
     3841 catattcatt atatatcaat tcaacattgg atatattaaa tgttgaatta ttttctacct
     3901 ttgttgatgt atctacttta tcaccttcat ctatatttat aatttctgat tttcctatat
     3961 tcaatacttt tgcatttccc tcatctattt ttatctcatt tttatcttct gtttgaatat
     4021 caactgaatc aggctgttca tctttttcac atgctgtcaa cataaaaata aatattgtta
     4081 atataatcat tagagaaatt gattttttta ctattttatt catattaagc acctcatata
     4141 gtagtatttt atagtagatt tttatacttt ttcataaatt ctttcaatgc ctgtgtaagt
     4201 atttgatgtt tttcaaaatt cgaattacta cataatcttt caaaatcatt ccatatttct
     4261 ttatctatac gtattcttgt tgtttttaat tcatctatgt gttcatcatc tatatgtaca
     4321 tcttttttat tagatacttt tttaggcttt atattgtaat cttttacttc taaataccaa
     4381 tcatatactt cacaaagctt atctaaatct tcttgagtta tatttatttt tctatctttc
     4441 ttaggcttaa tattttgttt tggctttgca ttttttaaac ctgtatcatt aaactcaatt
     4501 tgttcaacac ttttacttaa agccttttca ttagaaatct tttttttggt gcttactttt
     4561 tttctattac ttatagtatt ctttttactt ttttcatact ccttagcttt ctcttcttcc
     4621 aagtttctat tatccaaact tttattataa tttaattcgt cttttaatat gtatttacct
     4681 ttttctgact tatatccttt tttatttaaa aaagttctta aagtactttg actaatcttt
     4741 agattttgag ctatctctgc aaatttaaat cctttgcttt gaagttctaa aaatttgtca
     4801 attctgtctt cattgttatt cattaatatt cctccgtcaa catcttcttg tctttctttt
     4861 catacttctt attatattct tcttttctaa atctactcta tttgattcaa ttattttttg
     4921 tagcatttta ttataagtga aatcatctaa attattattt tttatataga gaaaagtttc
     4981 ctcttcacat ttaataaaac aaattgatag caaccatgcc atacccattt taacataata
     5041 ttcttcactt tgtatattat tacagtattc aaatatttct ttaatatgtt tatcatcaac
     5101 ataatatatt aaaaacataa ctaatgcaaa tctaatttcc catggattct ttgaataaac
     5161 atatttttta agtatatcat acatattttc tttatatttt ttcatagact ttaaatcaga
     5221 acaaaatcca tcgcaaactg accaattatc gattttaggt ataaatcttt ttacatagta
     5281 taaagtttct tcaaaattac tatttatatt gccaattacc ataccttgta gcctaacttc
     5341 ttcatagtat gtatcctgtg ctacttctaa aaattccttc caattccctt ggcttatttc
     5401 ttttgataac tttcttaaat taggaactct aatacctatt atattttctg ttccaggtat
     5461 aagcttttta ttaaaatctc tatattttat atcttgtata gacatcatat attctaaaag
     5521 ttcattataa tccaatttat tccattttcc cctatcaaac atcatttaat tgctccttaa
     5581 ttcctttttt cttattgtaa cataaaactc tacaaaaaaa gcttctaact attagaagct
     5641 ttttcaaaaa cataagaaac ttttcaattt taaatttaaa ctaaattaat gctaatctcc
     5701 tacaccaaag tctgtattat tgtataaaac ttctatattt ggattttcat ccaagaattt
     5761 atacttacta acaaaccatt tcactaactc ttcatctctt tttatactag aagaattatt
     5821 tataaaaaca ttcactgtcg ctttttcaaa agtatttagg ataatctcta tatctttatc
     5881 tatcatctct tttgtttgtc cttttattcc aaccataata caaggtgaat caaaatattc
     5941 ttttacatct tgtggagtct taaaatttgc atttttattt aaaatattat ttctaaaatc
     6001 attgtcaaag gtttcaactc caattttaaa taccacagga atttcaaaat actctctcat
     6061 ttccttaagc ctatttttgt aagaccaatg actttctaaa aataattttt ttatattctt
     6121 ctcttttata atacacttta ttttttctaa agtatcctta ggtaattcaa aacagcttcc
     6181 agaatttatg acttcaagaa ctccatattt cccagttacg ttttttaaaa cctttagatt
     6241 taatttattc atctcttctt ctaaattaga attatcatct atataatcac aaaaagaaca
     6301 ctttccccat atacaaggaa atcctttaag cagaacaatt tctctttgat ttttatttgt
     6361 tatctcacta tatctatcca ttttctatct attttcctcc agtcaatgat acctttaaag
     6421 tttttggaac tgcatttaat cctaaattca ctaaaacaac tgctaacagc ttatctccat
     6481 aatcagtaaa tacttgggtt acaaacacac taaaaacatc ccccagtcct aacactttca
     6541 agatttgcac tatatatgat gaacctgaag aggtcatacc tccaaataaa aatgctgaaa
     6601 ttattgaact tattatagat gttggaatgg caaataaaag tactcctagt ggagttttta
     6661 aaccccttaa aaaattcttt ttaaatgcta ttccagcaag taatccggtt gatatttgaa
     6721 ctggtgcaaa atagagagaa tacacatcaa acgtaattcc acttattaaa cttccaaaca
     6781 cacctgtaac aacagcgtat ttaggtccta aaagtgaagc tatcattata gttcctattg
     6841 aatctaagta tacaggtaat cttaatccca tagctataaa agcgcctatt atatttatag
     6901 aaataccaaa agctataagt gtaatagttt ttacacatat ttttcttttc acactatatc
     6961 actccttcca cagagtctat aatgtcttca taacctttaa aaagcctctt taaaaacatg
     7021 tacataaatt tcttctcatc aacttcattt agaacaattg cattaggatt ttttttatag
     7081 aaattgaaag aatccactat agactgaccc atagctattc catcttctac aacttctaca
     7141 tatgattcaa atcctttaca tatacttcta tctataaagt atgctactgc tagagggtca
     7201 tttatcacac agccaattat tccctcctgt tcccaatgga aatcaatata aaatctagtt
     7261 atttcagtta tatactttgc catcttctta tcaagtctat ttataaactc aataatatta
     7321 ggagtaagta caatttttct agttacatct aaacctacca tgtggatttt tttagataaa
     7381 ttcttgtaaa cataatctgc tccatgtggg tctacccaat aattaaactc tgctactgga
     7441 gagcaatttc catgaatcct aaatgctcct cccatagata caaactcatc gagattttca
     7501 aatgctttct tatctttaat aagtgcttta gctatatttg taagtggtgc aagtgctatt
     7561 attgatactt tttcatgatt atataaagta tttattataa aatccactgc tccatttttt
     7621 gctttagctc ctacaacctt ttgataaaag ttttctccaa taccatcttc tccatgtgta
     7681 tcttgagctg ttacaagttt tctttttagt ggtgcttctt ctcctatata tactggaata
     7741 tttagtgaag aacacatttg aagtgttttt agtgcatttt ctgcacctat atttgctggg
     7801 acatttccac aacatgtggt aattccaatt acttctagct ctggtgagtt taaagccaga
     7861 agaattgcca aagaatcatc aattcctggg tcacaatcaa ttattacttt tctcttttcc
     7921 attttttatc ctccttgatt tgtggtcgag gagaactaca catctttttt ccactttcaa
     7981 aaaggtgtgt ctaagggtat taattatata ctgccactat ataattcctt agttttttat
     8041 acttggagtt ctcgaacaag tacagatgta aaatcatatt tttaggctac ttactataag
     8101 actcttaaaa actatagacc gttaaaaaat ttttaaacat ctgatttaat agctataaaa
     8161 taaattttat agcataaaag aatttattat attttaatgt atcttattaa agtttattta
     8221 gaattatgaa ccaactttaa taagatgtgg cgcatttaaa ccgattatta ttatacaata
     8281 atatcttaaa tattacaatt aataacatta atttattatc cataaaaata tattaactta
     8341 tattatttaa taatttattg acagtattaa aattaaatta tacaataaca gtgtaaatta
     8401 acttaacacc gttaagcaaa gaggtgatta tatgagtatc aaagaaagac gtaaaaatga
     8461 aaaagaagaa atgaagaaaa agattatgga tgctagcatc gaaattataa accaacatgg
     8521 atatgaaaat ttatcaataa gaaaaattgc tacaaaaata gaatattctc caactacaat
     8581 ttatctttac tacaaagaca aagcagagat tatcagcgat atgaccaata aactttataa
     8641 cacagtagaa agtaatgcca ttgacattat gaataattgc tcatcactac caatagataa
     8701 acaaatcata gaaattatga ctagctttat aaaaactctt tctagtgaac cagagatggc
     8761 aaaatctatc atgcatagta gaatgaatat tatatttgca tctgaaaata caaacaatac
     8821 gcctagcaat aatggtataa aaatgcttga taaattcctt tccattggaa ttgaacaagg
     8881 aatattcaaa aaaaatatag ataaaagctc atggatgctt ataagtgcat tgttaggatt
     8941 cgttttatgt gttgttgaaa ataaactata ttctttacat aatttttctc aacttataga
     9001 aaatttctta gatattttag tgggaggact ttacaatgaa aattcgcaac aaggctacta
     9061 ataaaaagaa aaaatatgta aaaatatcta ttgctctatt ctttgtattt atatgcatta
     9121 ttattgtaat tgtaagcaac ttaaagccag aaaacttggt agtaaaagat atagatatta
     9181 ataatgttaa aaatggcatt tatactggaa gtgctgataa taacttggta aaagctactg
     9241 tgtctgttga agttaataat ggtaaaatcc aaaatattaa tatcttaaaa cacgaccatc
     9301 ttttaggtaa acctgctgaa aaaattacaa caagtattat taaacaacag tctttagatg
     9361 tggatgctat aacttctgca acttatagca gtaatactat acgaaaagct gtagaaaatg
     9421 ctttaagaaa gggagaataa ttatgaatac tattattata tattcaagta aatatggttg
     9481 tacaaaagac tgtgcaaata tactaaaaaa taaactatca gataatgtca ctattgttga
     9541 tattaataat aataataata aaattgagtt atcaaaattt gataaaatta ttattggaag
     9601 ttctatatat gttggttcag tttcaaaaaa aatacaggtg ctatgtaatg ataatgtaga
     9661 gttattaaac aaaaaacaag ttggcatttt cctttgctgt ggcttttctg aacaagcaga
     9721 taaatattta aaatcaaatt ttccatcatc cttattagaa agtgcaaatg ctattggaat
     9781 ttttggtagt gaagctcgat tagaaaaaat gaaattttta gataaactta ttatgaaggc
     9841 tgtttcaaaa ggtaattatg acagttttag aatatcacag gataatatag ataatttttt
     9901 aataaatctt aatagctaaa ataaagctaa ttatttctat gataaattct tttatttata
     9961 agtaatattg ctcaaaatag tctttaaact ttatcaaaat tttatttttc ttagtgaaat
    10021 ctaatgaaac tagcacttaa tatatgcttt attaatcata acaaatatgt tttattaatc
    10081 ataacaaaaa gagagtgtct aatgaactaa aaaattcatt agacactctc tttttgtatg
    10141 aaatcaaata tatttttatc atttcaacag agaaaaatac acttgtctct atgtttggca
    10201 cattctcttt ttataatata atgtttaatt taaaactact atttcaaata ttgacatata
    10261 cttaaactat agacttacaa aatcaattca tattagctta aaatataatc tttgttattc
    10321 aaattcctat acacaaccat tatttacttt gatactttaa aacatcttcc ctatttttta
    10381 ttaaatttta taataatagt cctattaacg caaatactat aaaaagagtt taactttatc
    10441 cattaaataa atttaaatta aaacatatag aaatggaagg agaacaattt atgataaaac
    10501 taatcggtat attgatagtt gtaattggat ttatacttaa aattgacaca ctatttactg
    10561 tattattagc aggagtggca acaggaattg ttgcaggatt agattttaat caaattttta
    10621 caattttagg agattctttt gtctcaaaca gaggtgtatc attattcata ttaacattgc
    10681 cagtaatcgg tgtattagaa agatatggtt taagacaaag agctgtttct ttaatagaaa
    10741 aattaaaaag acttacaaca ggaaaagtac ttactatata catgatagct agacaaatag
    10801 ctggtgctct atcaataaga atgagtggac atcctcaatt tgtaagacca cttgttaatc
    10861 ctatggcaca agctgctggt ttaagtaata gtgatgaact aaaagaatcg gatgaagaag
    10921 ccataaaggc attatctgct gcatcagaaa actatggaaa tttttatggt caaaacttat
    10981 ttgcaggtag tagtggagta ttacttatag catctactct gactcaattt ggatacaatg
    11041 taactggact taatatcgta aaggctagta ctattatggc agtaatagca cttgtagtgg
    11101 ctacaatcca gtttacctta tatgataaaa aattaaacaa aagtcataaa aaatagtttt
    11161 atttaggagg aataaaaatg caaaatttta taaacatatc attggaaatc ttttacgcac
    11221 taatgggatt tttaatgatt gttatagcat ataagtcttt tactaccata aataacaata
    11281 agaaatatgg tacatcatta ttttggatac ttatatcact gccattcata tttggtagat
    11341 tgattccagc caatataata ggaataatat taatattatc tagtttatta acactttcta
    11401 aacaagttgt atttgcaaag tatgaagaac cagatgaaaa ttttggaaaa gaacaagcag
    11461 acaaacttaa aaataaaata tttataccat cattaatatt agcatttgct gcagtagtag
    11521 tagctatgag tctttctaat tttgataatt cttctcagtt tgcaataggt gtgggttcaa
    11581 taattgcatt aatatcagcc cttataataa ctaaggcaaa acctgctaca tcagttcaag
    11641 atggctcaag attacttcaa caaatgggac ctgctagtat gttaccacaa cttttagttg
    11701 cattgggagc tttatttact caagccggag tcggtgaagt tatttcaacc atgatatctg
    11761 gtgtagtccc tgctgatagc agattatttg gtgttatagc ttatgtatta ggtatggtta
    11821 tatttaccat gataatggga aatgcatttg ccgcattttc agtcataacg gcaggaattg
    11881 gtattccttt tgtattgtct caaggtggaa atcctgctat aattggagca ttagcattaa
    11941 cagcaggata ttgtggaact ctacttactc caatggctgc taattttaat atagttccag
    12001 cagccttgct agaatgtaaa aatgattata ttgttataaa atatcaagct ccagttgctt
    12061 tggtattaat tatagcccac atattagtaa tgtacttttt aggattttaa tataattagg
    12121 aggtaaaata atgaaaattt tgttaacagg atttgaccca tttggcggag aaccaataaa
    12181 cccagctcaa gaagcagttg aaagggtcaa taataatata aacggtgcag aaatcataaa
    12241 gataactata ccaacagtta tgacaaaatc tgttgaagct atagataaag ctattcaaga
    12301 gcacaatcca gatatagtaa tatcagttgg acaagctgga ggtagatttg acattacacc
    12361 tgagagagtt gctataaata tagatgattt tagaataaaa gataatgagg gtaaccaggt
    12421 tatagatact attataaaag aagatggtga acctgcatat ttttcaaaat tgcctgtaaa
    12481 agctatggta aaacatatga atgaaaataa aattccagct agtgtttcaa acactgcagg
    12541 aacatttgtt tgcaatcatg tgatgtatgg aattctttat atgatagaca agaaatatcc
    12601 taatataagg ggtggattta tacacatccc atatacaact tcacaagtaa tagataaaaa
    12661 aaatacacca ttcatgtctt tagaagaaat agtaaaaggc ttagaattag ctatagaagc
    12721 ttgtattata tataaagaag atgtaaaaga aattggtggt gaaatttcat aataaatttt
    12781 ttagtatttt tataaattaa aaatactaac tattaattta aatatagact gtgagtagat
    12841 tattcaaaca attttactca cagtcttttt atggtatcta cagatacaaa attaaggtct
    12901 attcctctct ccccactcac acattgcatc taaaattgga atcagagatt ttcccctttc
    12961 tgatagacta tattctacct taggaggtat ttgaggatat tcagtacgaa taatcaagtt
    13021 atctgcttct aattctttta atgataaact caaagtttta taggagatag tatttatata
    13081 acgttttaat gcattatatc ttacaacttc aaattctaca agacagtaaa gaattatcat
    13141 cttatattta ccactaatca gtgaaagtgt ataactaaat cctgtttcac ttaaatctgt
    13201 atttgatata cagtctatac tcatatatta cactctcctt ttggttagta ttgaacaaaa
    13261 atgtgcgtac ttgtttttag aataatttat aataaaatta tagtgtaaga ataattgaaa
    13321 gtcaatttta taataggagg tgtatttact aaaaaaaaag ttattttaaa taggattcta
    13381 agattagaca tttctatata aattgataat attcatgaaa atcctatttt ataaaaaata
    13441 atgtctctta gaaaatctat taaataaaat tcattggagg aaatttataa tgaaagtttt
    13501 attgataaac ggtagcccaa accaatatgg atgtacttat acagcactaa atgagattac
    13561 taaagtattg agtaaacacg atattaaaac agaaatactg tatttaggta aagaaacaat
    13621 cccaggatgt attagttgcg catcctgttt tgaaactgga aaatgtattc gaaatgataa
    13681 agttaatgaa ttgattgaag atctggataa tattgatggt attattattg gttctcctgt
    13741 ttacttttct agtgcaactg gtcaattaac ttcttttctt gaccgtctat ttttcatagc
    13801 tgggagtcgc atggctacaa aattaggtgc ttcagttgtg tcatgtagac gtggtggtgc
    13861 aagtgcaaca tttgaccaac taaataaata tttttcaatc agcaatatgc ctattgtatc
    13921 atcacagtat tggaaccagg tacatggctt tactccagag gatgttatga aggatgaaga
    13981 aggattacag actatgcgaa ctctaggaga aaacatggca tggcttctta agtgtatatc
    14041 agcaggtaaa aaagctggaa ttaaagaacc acaatatgaa gaaacaatta tgactaattt
    14101 tataaaataa ataaaaaatg tactaatatt aattaaaaca aaacactaat ttataaataa
    14161 aataagcata aatgtaggat aaaaagcact aaaacagagg tattgaattt tatatcaaac
    14221 ctctgtttta ttattacatt aataaccaaa ccatgtataa catcatatat ttctaaataa
    14281 attataatat tatattctaa tgttattgaa attacaataa ctgaatttct gctcataaat
    14341 ttaaatattc tacaaatctc atctaaataa caattttaat agacctaaaa caatcctcat
    14401 actgttcatt ttgtattaaa aatatcactt acaatcaaaa accatttatt aagtatattc
    14461 ctaagatata caatatatat acatggattg gatagtataa ataaaataaa tacttcatac
    14521 tcttcccttt ttctccattg taaagtaaca ttaaaggtag agaagcaatc atcatccatt
    14581 ggtaattttg taaaatagaa ttttttaaac tataatctcc atttgacatt gttgtaaata
    14641 taaatataca cattaaaaca taacaaatac tcaaactgaa ctttttacct ctacacaaat
    14701 aaaaaataat cccaagtata atccaaattg gtccaccttc tactaaaaat ggtactggta
    14761 ccaaaatcat aaataaagca ataactctgt tagtgacttt aaaaagtaaa gctactgtaa
    14821 atattcctaa aattatagga atgattatca tcaataatcc tataataaaa tatttaattt
    14881 ctttctcttt tctaaatttc tttatgaact ctattgcttg taaataaatc gctattaaaa
    14941 ataatgttga aaaagtgtta tttataatta tagctccttc tggatgtgaa aatacattat
    15001 taattatttg atttcctata gccataataa cagaccaaat atacaatcta cttatatatt
    15061 tttttctgtt actagtgtga taaaatcctt caacagccat gaatataaac agtggtgctg
    15121 atattctgcc tacccaatga aaccaaattg gtatttgcat tgtttgtggc ataaatgctg
    15181 ctatatggtc aaatgtcata aatattaatg ctaaaatctt aatagtaaac ccacttatac
    15241 cttttttaaa aaaatccata tcatatctcc taattttgtt ttatctctat aataattttt
    15301 gttcctattc caatttcact acttacataa attttacctt tatgaatctc tattatttct
    15361 ttagaaatag ccataccaag acctgaacct ttatgcatct cacctgtatt agtacctcta
    15421 taatattttt taaaaatatg tttcaaatca cttttgctta ttccaattcc atcatctttt
    15481 attataattt ctatattatc tttttttaca atttctacac ttatcaaagt tccttcgcta
    15541 ttatgaacta ttgaattaaa tataagattg gttatagccc ttctgaataa gattgaatct
    15601 acatatactt caattaaatc ttcattactt tcaaattcaa tattcctatt tttatatcta
    15661 ttatcattta aaatatcaat tattatatct ctgactaaac tgactaaatt tatttttttc
    15721 ttgtccagaa ctatagtatt attttttaat cgagtagata aatttaaatc atcaactaaa
    15781 tcttttatat acttagactt attatatata atttctgtat actcttgtat ttcatcttct
    15841 tcaaactcat aatctttgtc attaattatc tctgcatacc cttgtataga tgctagaggt
    15901 gtctttatat catgtgttat atttgccaac cattcctctc tcaactcttc attttctttt
    15961 ctctcaactt catttactct taaagtatca gctaacatat ttatattttt aaaaacttct
    16021 tcatatatac ctctgtcctt aagatataaa ctataatttc cattagccat agtctcaata
    16081 ctccatagta cgcttgaaat tggttttgta agttttctac taaataaata tcctacacct
    16141 aaagctacca cgctatcaat tactagtgtt ataatgataa ctttatttaa agttttttta
    16201 atgttatttt gagaataggt taaaattatt cttgatatat ctctactagg tatacctact
    16261 attatgttta gatgtttttt gtttaaatat ttttctaata caaaattaac atactttgtt
    16321 tctatatact tgtaattatt caccatttga aagggtgtat attttttggg agtatccttt
    16381 ggcacattta ctccataaac ttcctctcct aaatcattta aaacctgtat ccaggaattt
    16441 gatttttcta atttttcagc tccaatctta gatagtttaa attctccatc tttttcatat
    16501 aaatcttttt caaaagaacg tgcaaattct tcaggattat ttccagaatc tacaacttta
    16561 aaaaaactat tagttgaaat cacatacaat atagatatga tatttataat tactactgat
    16621 atagctacaa acacaattgt aaatataaaa tttctagtaa tcttccactt catattctaa
    16681 tcttccttta ctgctaattt atatccaaga ccttttattg tcagaatata ttcaggcttt
    16741 gaaggattat cctctaattt ttccctaatt tttcttatat gaaccattat agtattgtca
    16801 aatccaacat aatcttctcc ccacacttta tcccatagtt tttctttact tataatttga
    16861 ttttgattat gtgcaaataa agataggatt tttagttcct ttgcagacag tcctatagat
    16921 tttccatctt ttataagttc tgccctactt tcatttaata tatatgggcc aaatgatata
    16981 attttttctt cattattatt tagattttta ctttcgtttt gagcatcact aaaataactt
    17041 aatcttctca aatgcgcttt aactctaaaa gccacttcct tagggctaaa aggttttgta
    17101 acataatcat ctcctccaat cgctagtcca agtaacttat ctaattcttc ggttttagct
    17161 gataaaaata ttattggaga attggaggtt tttcttattt ctttacaaat atcataacct
    17221 tcgccatctg gtaacataat atccaataca atcaaatcag gattaatact tttaaataat
    17281 tctaatcctt cttttttagt ttttgcagta tatatattat taaggccctc tttttttagg
    17341 acagtttcta aaagcttcaa tatttctact tcatcatcta ttattaaaac acgattttcc
    17401 ataaacaacc tcctgtaata atttacatat atattagcat atattttatc taaaattcat
    17461 ttatcttatt tttaattaaa ctaaaatata aataaaatta ctattatttt tagttaaaac
    17521 cttttgcttg atgtaaaatt ttcatatgtt attttagtta acactatcat taatataatt
    17581 gagattacta tagaaaaaac tatcaaaata cttggtacat tatacttaac catttgttgg
    17641 ctcatcataa tccaatttgg agtcactgac ataaatttta tagctttatc tggtataaaa
    17701 cctacactaa agtatggaat tgccattatc aatccaccta gtataaatgg aattacagcc
    17761 gatttaaata tagatgaaag tgttaagcct atttgtgcaa atataaaaga agcaaaaata
    17821 gtagtcaata taacaattat atacatttgt attactgtaa gatttaaatt actatttcca
    17881 aagtaccata agttatgaat cgattcattt aaatttttaa aatcaaaaaa actaaaacct
    17941 ctaattccta ctaacaatat aaaagaaata ttaataatag ataaaattaa tccattagct
    18001 actagttttg ataatactac tgattttttt ccctttttag ttgtatttat taaacctgtc
    18061 atattagaat catattcttt aatgtaattt ccagaagtta ttataaacat tatcaaacct
    18121 attagaggta ttgagccttc tatcttgtaa aatatatctg cataagtatc taaaacatca
    18181 aactttctaa tcagaaaaaa tccaaaaaat attaaaaaca gccaaattat ccaaacactc
    18241 tttgatttta atatttttat catttcagct ttaactaaat acatcatttc tactacacct
    18301 cccttgcatc ttctaaatca aaataaaaca tgtatacatc ttcgaatttt gcttgaactg
    18361 gttgtatatt atcctcgctt ggaatatcct ctgaaacaat tctaagtact actgaatcaa
    18421 tatcactttg aatacttaca accttatatt tagattgtat atcatcaact tcacattcat
    18481 ttttaacttg aacattatat actttatttt ccatgtgact taatatgtct ttgtgtgttc
    18541 ctttcattag gagtttgcca tctttaatca ttattgtttc ctttgcaact gattctatat
    18601 cggaaattat atgagtagaa agtattatta tcttatctct agacattttt gctattaaat
    18661 tcctaaatct agctctttct tgtgggtcaa gtcctgctgt tggctcatca aggactatta
    18721 tcttagggtc gtttagtaaa acttgtgcta tcccaactct cctcttcata cctcctgaaa
    18781 attttcctat agacttattt ctcacatcat aaagacctac taaatctaat aattctccta
    18841 tcttactatt taaatatttt ccccttaaac cttttaaagc tccaatatac tgtaaaaatt
    18901 cttttgctgt aaaattttta taaactccaa attcttgtgg caaatatcct aaaatatttc
    18961 tataatcagc atccatttta aatatatcct caccattata aagtatctga ccttcatcag
    19021 gttttatcaa cgttgttatt tgtttcatta atgtagattt tcctgctcca tttggtccta
    19081 gaagaccata aactccattt tccagagtta ctgtaatctt gtcgtttgct acttttttac
    19141 catatatctt tgaaagattt tctatttcta atttattcat cttaactccc cttttatcta
    19201 cttatataat ctgattttat tactaaaaaa cataatgaca tagcacctat caatattgcc
    19261 atcaaaagtc ctattgctat attgacaatg tctatattca ctttaaatat cataatatca
    19321 cctagataat ttcctattat tttattaaaa ccaaatatca aatcataata atttacattt
    19381 gatacaactg aaagtaattt tttaggtaat atatggatta ccgacattat cttagttata
    19441 gatataataa atataaaaaa acttattgaa gatattgaag acttacttat aaaagaccca
    19501 aatatggcta tcatggccca tccttcaaac atcaaaagct ctatgcctat atttaatatt
    19561 acatactttg ttatacttaa attagttttc aatgcaacta cattgtctac tattctaaaa
    19621 gattctaagt ctccattaac aggtactcct tgttgcatac aagttataat aaatattccc
    19681 attaaataaa cagaataaat tactaatgga actaataaag caacaaatat tttagacttt
    19741 aatgcttttt tcttctcttt tgatgatagt atgatatctt ttaccgaagt aacgacttca
    19801 tctacataaa tattggatat aattatagat ataatgatta acattcctac atttataagt
    19861 gaatttgtga ctctatagtt aaatactttc caaaaactaa tatccttata ttcattcaca
    19921 cttagattat caagtttttt ctttgacatt aattcaattt ttttagaaaa ttcacctgta
    19981 tctgtttgat ttgaaaactg ctttaaaaca ttaatcttat tttgaaattt ctcattacct
    20041 acgtcgatac cacttctcgt atcttctata cgtccttttt ctttatctat ataataactt
    20101 tctgtttcta aaacaggcgt aataaagata ctaatcatta gtattaacac taatatccct
    20161 agagatataa acatactctt agatttagcc aattttttta tttcccaatt taccaatata
    20221 tttctcctcc taaatcattt taattacaca tatatattaa aacttaaatc ttatataaat
    20281 ataatactaa ccttaaataa agcttaaatt attttataat ttataggtgt gtttattatt
    20341 ggagttacat ttattatttt tctgggaaat tatacatttt caattatttt tattattttt
    20401 gatattttta ttgattggaa attttattgt tgttcaaatt gtagttattc tttaaattta
    20461 aagatggagt tttttaagaa tagaacctac tgtccttatt atggaaagat tattaaatag
    20521 ttgcatataa ctttatttat cttgaatctt atttttaaaa acaattataa ttttagaata
    20581 ttttctattc tcaaacaagc atgttgaata atggagcatc ttctagtatt atatacttgt
    20641 agaataaaac taaattggta aaactgggaa tatttaacct taaggagtta agttatagaa
    20701 actagtacta aaaaatttta taaattatgt taattgacta atagaatgcg actttttatt
    20761 tttactaaat tttttagggg gatggataat atgtttattg gagaaataga caaatgtaca
    20821 catatactaa ctgcttacat cagtagttca tatgattatt gtaatttttt agatacacag
    20881 ttagatgatt ttatatcaga atatggagaa acggtagttg aaacctgctt acatcaagtc
    20941 ttattattag taagtagata caattaaaac taaatttttc tagaaaaacc tttttagatt
    21001 tatctactct aataaatata taattttttg tactttataa gtaactataa cttctgctaa
    21061 agtaaagatg tattgggagt acatctttac tttcccatta tagaagttaa aagcatacac
    21121 cataaaatta atcatccaat acaaattata tatttcctat gtctaactta atttacaaga
    21181 atcattctat atgcatattc tgcaatccac taaacatggc tctaaacctt tactatttta
    21241 tacattctct ataaatcata caaatcatat ataaaatccc cctaatatgt ttaaaccacc
    21301 tataaaataa caccaatatt tagaatattc aatatcaact gcataactgg tcaataaaaa
    21361 cgtataacta gacaaatttc aatatttata taaataatac aagaaaaaca agcattttat
    21421 ttatataagt tttttatttt ttgtttatat taaaaaacta tattgattaa tttattttat
    21481 ttagtaatta taaaaattcg tcttagaata tattctatat ttgtagatta actttaatat
    21541 ttttaatttc aca
//
LOCUS       DAYAPA010000066        20707 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.066, whole genome shotgun sequence.
ACCESSION   DAYAPA010000066 DAYAPA010000000
VERSION     DAYAPA010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20707
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.066"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            411..1304
                     /locus_tag="ACGI59_002249"
     CDS             411..1304
                     /locus_tag="ACGI59_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HFG9740035.1"
                     /translation="MSNKIVSKRQLTDSIYLMEIEAPRVAKSSQPGQFIIIKNDEKGE
                     RIPLTIADYDREKGTVTIVFQTVGASTKKLAMFEENDFVMDFVGPLGQASEFIHEDIE
                     ELRNKKILFVAGGVGSAPVYPQVKWFKEHGLDVDVIIGARTKELIILEDDMKKVAKNV
                     YVSTDDGTYGFNGRVTDLLKDLVDNQGKKYDQAIVIGPMIMMKFMCQLTKELNIPTIV
                     SLNTIMIDGTGMCGGCRVSVGNETKFACVDGPEFDGHLVDFDQAMRRQSMYKTQEGRA
                     MLKLEEGDSHHHSNCGCGGNK"
     gene            1304..2698
                     /gene="gltA"
                     /locus_tag="ACGI59_002250"
     CDS             1304..2698
                     /gene="gltA"
                     /locus_tag="ACGI59_002250"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HFG9740036.1"
                     /translation="MDAKKVKVPVREQEPAVRATNFDEVCLGYNKEEAMAEANRCLAC
                     KKPKCVGGCPVGIDIPGFITKIKEDDIEGAAKVIAKSSSLPAVCGRVCPQESQCEGVC
                     ILGIKSDAVSIGKLERFVADWSKENDINLSDTEPKKNQKVAVIGSGPAGLACAGDLAK
                     KGYDVTIFEAMHEPGGVLTYGIPEFRLPKQAVVQPEIDNIRKLGVKIETNVIVGKTIT
                     VDELIEDEGFEAIFIGSGAGLPMFMNIPGENANGVFSANEFLTRVNLMKAYRDDYDTP
                     ISSGKKVAVVGGGNVAMDAARTALRLGSESYIVYRRSEKELPARAEEVHHAKEEGIIF
                     NTLTNPKEILVDENGYVKGMVCIRMELGEPDDSGRRRPIEIEGSEFVLDVDTVIMSLG
                     TSPNPLISSTTKSLDINKKRCLITDENGQTSKEGVFAGGDAVTGAATVISAMGAGKTA
                     AASIDEYLKAKVNA"
     gene            3064..5283
                     /locus_tag="ACGI59_002251"
     CDS             3064..5283
                     /locus_tag="ACGI59_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFG9740037.1"
                     /translation="MKKREKVFDGLVFKHILILLILLVLIDLTSTIIYASNSMEITSR
                     NMIEASKRELENYLEVNISLLKALSQDDRFSNGETSLIEKGKLLSPYQKEYNLFMIGI
                     TDTKGNASSTYRENVNSIKDKPSFEKAIKTKQVVVSDIEVSNVTGDKVFIIYVPIVKN
                     NEMIGTIFASFYFQDVNNLISRSNFDDSIKFLMIDKDYTIISHPNKKYVNDKSKMLDL
                     EGNIIGTTKSEILKNINKKCQGDFLSWDNWRLYNVKYTNIKWTNWTLVSKCNIFKNFK
                     NLIVNFMIKLYFYIVIFMILWKLSNAKLIEQLKKLAYYDSLSGIKNKEKFRKDSMYIL
                     KNYYQDNFYLVQLDVNKFKYINEMFGYAEGNKILIHISQVLNNNTNKYEICARMDNDH
                     FILLIACNTEDELLNRLSKINKEICNLNTTNSSKYKIVMSSGIYKITKKDDIKKIDLL
                     IDRANIAAKSKKEKYEHSYSFFNEDTRNRLYKEKRLEDNMNKALEKGEFIVYYQPKYS
                     LDDVNEIEGAEALIRWNSPEFGFISPIDFVPLFEKNGFIVNIDMFVFEEVCKTLNKWI
                     NKGYTPVPISVNMSRVHLYRDNFIENITDLISKYNISPEFIELELTESVVFDNLNILI
                     DIMKKIKEIGFLISMDDFGSGYSSLNLLKDLSFDILKLDRGFLIETTDTKRGKIIISK
                     IVEMAKAIDIKVICEGVETYEQVEFLKEIGCDKVQGYLFAKPMVLDEFEKHLNFKFD"
     gene            5430..6176
                     /locus_tag="ACGI59_002252"
     CDS             5430..6176
                     /locus_tag="ACGI59_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9740038.1"
                     /translation="MNILSIRNISKTYCGNIPFKALDKVSLNIEKGEFVSVMGPSGSG
                     KSTLLNIISTVDRQSEGEVVLDGYDVSKLKGEKLAEFRRKQLGFVFQDFNLIDTLTVG
                     ENIMLPLTLEGASIKDMNIQTKSISKFLGIDKILDRKTYEISGGQAQRCAIARAIINK
                     PAILLADEPTGNLDSKSTDDVLKLFTRINKEQKVTTLMVTHEAYSASYSDRVIFIKDG
                     CIYTEIKKSESSNSFYSDILAVLSQIGGVR"
     gene            6176..8056
                     /locus_tag="ACGI59_002253"
     CDS             6176..8056
                     /locus_tag="ACGI59_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9740039.1"
                     /translation="MDFRKFAYNNIVKNFRAYLAYFLSSVFSIMIFFVFAVSMFHPII
                     TKSGMQSGSTAFMALMTSELIIFGFSLLFILYSLGNFLKYRLKDFGVLMIIGMSNRQL
                     KKLILIENLIIGFLAVMLGILLGLSISKLFLLYLSKLFYMNLTEFYFPVKAMLLTIVS
                     FMILFLITGPMSLKLLSKKSILELLVGTKKPKKEIKSSKLFGIIGVICLILGYAMILF
                     GNKLKINGGMYTTALLITLGIFLFFSQFTILILKYLKNKEKFYKNKVNMLLISNLIYK
                     LKDNTRLLFLITILLSATLVAFTTTSTLVSSQGEASKNNFPMVYSYFSEHNNEKEYEE
                     LQQIREAIKGYDYKELSFPVLKYNREVLLSVKNYNKLSKELGLDKISSKLEKYEGVII
                     PSSNSQEHINSLKQLKNYSIKNLNIEVKKSKNKIIFPTGLFSKVVVISDDLYNEVKGE
                     FSKINFHGFDYKNWQSSAQITENLKEKHFNLDREYNRSYFLTLPDMYLVELQQSKILQ
                     FMGVFIGVILFIAVFSFLYFRLYTDEIEDNVKYKKLSKIGLSFKNMNKIVSIEIGTLF
                     FIPYFIAIINSIISLLFLNQVLNNSFKLENLGILLLISSIYSLYFYLLKKIYTRKVWF
                     FD"
     gene            8175..9566
                     /locus_tag="ACGI59_002254"
     CDS             8175..9566
                     /locus_tag="ACGI59_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFG9740040.1"
                     /translation="MENQQLLGTERISKLLLKYSIPAIIGMLVNSLYNVVDRIFIGNI
                     PGVGPLAITGLGVTMPIMTIILAFGMLIGIGTTTTISIKLGQGKVEEARKLIGNAMTL
                     SVITGIIIMILGILFANKILTLFGASENTLIYAKSYINIILLGTVVNLLSFSLNHSIR
                     ADGSPKISAGIMIVGCLTNIVLDWILIFGFNLGIQGAAIATVTSQALTAILTIGYYIS
                     GKSNLRFSKSNLKLDKKLIKAVFAIGMSPFAMQLAASLVQVISNIALKTHGGDLAIGA
                     MATISSIAMVFLMPIFGINQGAQPIIGFNYGAEKYDRVKKAYLGSLVVATIILCMGMV
                     VVMLFPEAIIGIFNKDPELMNISVNGLRIYLLMLPIVGLSVTGTNFIQSIGKAKMAML
                     LSLLRQVILLIPAVLILPTFLGLQGVWTAQPVSDFIATVITGIVVFRELKRYTPKTEK
                     LNENERLNEITTE"
     gene            9673..10713
                     /locus_tag="ACGI59_002255"
     CDS             9673..10713
                     /locus_tag="ACGI59_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HFG9740041.1"
                     /translation="MEFTKKSKLKNMYVNMNVARMLSKINEYKGRQLLYKKQPKEILE
                     NLEKKSLVDCSESTYIGNQRDSSNFNLEKLISNEVTPRSREELSIVEYRDVVKTINSA
                     YESIPISSQTILELHGYLYKFSSTRGGSYKSDNDFIEHNLKPSEFISIDSSVNKVEKA
                     VEEICEAYNTLIEEDEIDILILISAFVLDFILIHPFKEGNIKMARVLILLLLNKNGYE
                     VGRYISLGKIFDDSSYEYYSNLNYLKASIGSEKADMNAWIEYFLETILTAYEKLDDSL
                     NISDKKRQTKTSRIEKIINSTLGYFTKEDIRDLCPDIPEPTINRVFNNLRKQDKIEVV
                     ARGRSAKWKKKY"
     gene            11202..11855
                     /locus_tag="ACGI59_002256"
     CDS             11202..11855
                     /locus_tag="ACGI59_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFG9740042.1"
                     /translation="MKKKLLYINVNSKPEDLSSSKTVARKFINKFMEKNKDFEVEEID
                     LYKEHIPRLEYQYFEKRNSIVSEENAKNLDDKDRKELTKIRNLCDQFVSASVYVIAAP
                     MWSLSFPAPLKEYIDCIIQDGKTISFEGNDKPQGILNDIDRSMVYIQSSGGHIPWVLK
                     PVMNKGLNYVESIMKFIGIKKFDELLVDGTGTSEEERQAAIEKAAEKIDGIIDGMKF"
     gene            11931..12140
                     /locus_tag="ACGI59_002257"
     CDS             11931..12140
                     /locus_tag="ACGI59_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9740043.1"
                     /translation="MREEKSNEKYDCYWCNQENNFCVEIKDNIVMIDDGTGTLKQAVF
                     IGYKQIQINLNCSHCQNLNRIKLNL"
     gene            complement(12280..12846)
                     /locus_tag="ACGI59_002258"
     CDS             complement(12280..12846)
                     /locus_tag="ACGI59_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HFG9740044.1"
                     /translation="MMNNELNTIILETLNNADITSNDIPSIDLYMDQIISLIDNKLSA
                     NKRFESDKILTKTMINNYSKEGLIKPVKGKKYTKEQILQMIIIYSMKNTLTIQEIKRI
                     LHGVYEKDNFSEKDLVSCYEKFMLIKENQRKNIPDFIESNFENISINPENKDDLLIAL
                     LSLTSMADQLKNISEKLVDRYFPDITKK"
     gene            13007..13660
                     /locus_tag="ACGI59_002259"
     CDS             13007..13660
                     /locus_tag="ACGI59_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFG9740045.1"
                     /translation="MYQFFLKGRDPISSLTHFIGACLSLLATIILVFQSVTLQETSLL
                     MIVSVSVFGFSLIALYSASSYYHFLKGTPEQELFFRKVDHAMIYVLIAGSYTPICLNF
                     MEKKEGIIFVTAIWIVAFIGIIIKIFWMDAPRWLSTSIYLLMGWAIVFDINAFNSIPK
                     DCLRLLIMEGVSYSIGAIIYIIKKPNISPEFGFHEIFHIFIMIGSLFHFLAVLLYVL"
     gene            complement(13818..13994)
                     /locus_tag="ACGI59_002260"
     CDS             complement(13818..13994)
                     /locus_tag="ACGI59_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9740046.1"
                     /translation="MYKYDDYVNLTNDLDLPVEIRNKCVRIVSVSENLEDIIIVHKFK
                     LYVVNEKYIKGIME"
     gene            14522..15466
                     /locus_tag="ACGI59_002261"
     CDS             14522..15466
                     /locus_tag="ACGI59_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HFG9740047.1"
                     /translation="MKFLRIEDEVIYSKKRYKLKREIDKLFLDEEYFQIEPQLFEEYD
                     EFTTINNKIPEESMVKVVNGKVMVLRADITTSIIKSLVPRWEDGLKLKLFYNSSIYKN
                     KNTVGIKEIRQIGCEYLGEASVEADREVVKLALKILEKYNNNFILEVGSSKYIHGLLE
                     ELNLNKNCENQIKNLLYTKNTHELKVYIEELKIKREVKELLSNILSLQGNLYNVIEKS
                     NKFYCNNKMKQALEELKQVNNLIEECNFLDKARFDLSMITMLDYYEGIMFRGYYPNSY
                     KEILSGGRYDSLTKEYGKEIPAIGFTLSVDELMKYVYK"
     gene            15488..16114
                     /gene="hisG"
                     /locus_tag="ACGI59_002262"
     CDS             15488..16114
                     /gene="hisG"
                     /locus_tag="ACGI59_002262"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HFG9740048.1"
                     /translation="MDYLTIALAKGRIEGESFKKFKKMGLGDSIDTDTRKLIFKDEEN
                     KIIYIHVKPSDVVTYVEKGVADLGIAGKDTILENETDVYEIYDLGFGKCKFAVAGLKG
                     DSIYREDEYLKVATKYPNIAKKYFKEKGQKIEIIKLNGSVELAPIVGLSDVIVDIVET
                     GNTLKANGLEILEDICNISARIISNRASYRFKYEQIQNIIRLFEELDN"
     gene            16148..17197
                     /gene="hisC"
                     /locus_tag="ACGI59_002263"
     CDS             16148..17197
                     /gene="hisC"
                     /locus_tag="ACGI59_002263"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFG9740049.1"
                     /translation="MREKESIRELRGYEPNHVNCKVKLDANEGSKRLFKYLIKEISDS
                     DIDLNLYPEDSYSDLKESIIDYINISGVNKKNLLVGNGSSEIIDLIIHTFVDKDEVIL
                     SFSPSFSMYSIYSQINGSKFIGVESDENLVINIDNVIEKVKENNPKIVIVCNPNNPTG
                     TILKREEIIKLLDSTNSLVVLDEAYMDFGEESMLSDVFKYDNLIVLRTLSKAFGLAGI
                     RTGYMLSNSSLINSVEKVRPPYNLNSLSDFIATRALRNKDVVKAYIKEVKEEREVLYE
                     EMIGMGIKAYKSQANFILFYSEIENLSQKLIDRGVLIRKFGGKLENYYRVTIGDKEEN
                     SMFVGAIRDILKKEK"
     gene            17200..17778
                     /gene="hisB"
                     /locus_tag="ACGI59_002264"
     CDS             17200..17778
                     /gene="hisB"
                     /locus_tag="ACGI59_002264"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HFG9740050.1"
                     /translation="MRIWKVERNTLETQILVELNIDGSGKAEIDTGIGFLDHMLTLMS
                     FHGKFDLKVICKGDTYVDDHHSVEDIGIAIGEAFKNALGDKKGIRRYSNIYIPMDESL
                     SMVAIDISNRPYLVFNAKFDTQMIGSMSTQCFKEFFRAFVNESRVTLHINLLYGENDH
                     HKIESIFKAFARALKEGSEIVSNEIASSKGVL"
     gene            17780..18385
                     /gene="hisH"
                     /locus_tag="ACGI59_002265"
     CDS             17780..18385
                     /gene="hisH"
                     /locus_tag="ACGI59_002265"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HFG9740051.1"
                     /translation="MNIIVDYGLGNIDSVSRGFRKAGIETKISSDIDEIKQADSLILP
                     GVGAFRDSISALDKLGLIPIIKEHVSKGKFMIGICLGMQLLYEKSYEYGEYEGLGLIK
                     GSIDKLDISLKVPHMGWNNLKFNKANDDILKYINEDDYVYFVHSYYANSSNEELIAFS
                     EYEKKIPAIVRKGNVYGIQFHPEKSGEVGLNILRAYGEMIK"
     gene            18382..19104
                     /gene="hisA"
                     /locus_tag="ACGI59_002266"
     CDS             18382..19104
                     /gene="hisA"
                     /locus_tag="ACGI59_002266"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HFG9740052.1"
                     /translation="MIIFPAIDIKDNKCVRLTQGEFDKVNVYYDNPLEVAYKWKNEGA
                     EYIHIVDLNGARSEFGVNTKIIEDIANNIDIPIQVGGGVRDKEKVKSLINAGVTRVIL
                     GSIAIENLNLVEELVNEYKEKIVVSIDAKDGKVAVRGWEVVSNVDSLTLCKQLEKIGV
                     QTIVYTDISKDGMLQGPNFDIYERIAKETSLNVIASGGVTSIEDVKRLKAMNLYGAII
                     GKALYDKKIDFKEAQQLCLLGE"
     gene            19086..19844
                     /gene="hisF"
                     /locus_tag="ACGI59_002267"
     CDS             19086..19844
                     /gene="hisF"
                     /locus_tag="ACGI59_002267"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HFG9740053.1"
                     /translation="MLTRRIIPCLDVRNGRVVKGKKFKDIVDVDSPEVLGKFYSDCGA
                     DELVFYDITASNEERKTSLEFVTKVAENINIPFCVGGGVNKLEDFTDILRKGADKVSI
                     NSSAVKNPELIREASLKFGAQCVVLSIDAKKNEEGSWSVYVKGGREKTNLDAIEWAVK
                     GVELGAGEIVVNSMDEDGMKNGYDIELLSKITSLVNVPVIASGGAGKKEDFYEAVNKS
                     NVDGILAASVFHFGEIKINDLKKYLKDMGVEVRL"
     gene            19872..20540
                     /gene="hisIE"
                     /locus_tag="ACGI59_002268"
     CDS             19872..20540
                     /gene="hisIE"
                     /locus_tag="ACGI59_002268"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HFG9740054.1"
                     /translation="MDNKCNNVYSDEVEKFIRSIKFDDKGLVPVVVQEVVSKDVLMLA
                     YMNKDVIKKTLKDKVACYFSRSRQELWVKGETSGNTQKVVKMSYDCDVDTILLFVEQT
                     GVACHTGNYSCFYRDLFDDSSKMELEVQTNILKELYDLINERKNNPVEGSYTNYLFEK
                     GIDKILKKVGEESSEVIIASKNTDKSELIYEISDLVYHTLVLMIEKGVEIDEIKKELL
                     KRRK"
BASE COUNT         8221 a         1945 c         3650 g         6891 t
ORIGIN      
        1 aatagattaa agccttatac acgtttagtt tttaaagaaa ttagtgttat tatgaaaacc
       61 taagttaatt tattaaataa ataattgaat aaaaaaattt tattatgtat ttataaaatt
      121 tttgttacaa atttggcata atatagtata ctttaagtgt gtattaggtt aaatctataa
      181 caaattagac gtatgatata gacttagtgt ttattggcat ttacaagaaa tttgttaata
      241 tcaactattt gtagtttggg cagttataat tattataggg gggatgtcca atacaaaaca
      301 atcaaccaag aactaatatt atattataaa gagttatagt tttgtgtgtt tatgttttta
      361 attaaattta aagtgggaaa aatatatttg ttagaatgga gaatagcgag atgagtaata
      421 aaatagttag taagagacaa ttaacagata gtatttattt aatggaaata gaggcaccta
      481 gagttgcaaa atcttctcaa ccaggtcaat ttataataat taaaaatgat gaaaaaggag
      541 agaggattcc tcttacaata gctgactatg atagagaaaa aggaacagta actattgtat
      601 tccaaacagt tggtgcttca actaaaaagt tagcaatgtt tgaagaaaac gattttgtaa
      661 tggattttgt agggccatta ggacaagcaa gtgaatttat acatgaagat atagaagaat
      721 taagaaataa aaagatatta tttgtagctg gtggagttgg ttctgctcca gtttatccac
      781 aagttaaatg gtttaaagag catggattag atgtagatgt aataattggt gcaagaacta
      841 aagaattaat tatattagaa gatgatatga aaaaagtagc taaaaatgta tatgtatcaa
      901 cagatgatgg tacatatgga tttaatggaa gagttacaga cctattaaaa gatttagttg
      961 ataatcaagg taaaaaatac gaccaagcaa tagtaattgg accaatgata atgatgaaat
     1021 ttatgtgcca attaactaaa gaactaaata tacctactat agtaagttta aatacaataa
     1081 tgatagatgg aacaggtatg tgtggtggtt gtagagttag tgttggaaat gaaactaagt
     1141 ttgcatgtgt agatggtcct gaatttgatg gtcatttagt tgattttgat caagcaatga
     1201 gaagacagtc tatgtacaag actcaagagg gaagagctat gctaaaactt gaagaaggcg
     1261 atagtcatca tcatagtaat tgtggatgtg gaggtaacaa ataatggatg ctaaaaaagt
     1321 aaaagtacca gttagagaac aagaacctgc tgtgagagca acaaattttg atgaagtatg
     1381 cttagggtat aataaagaag aagctatggc agaagctaat agatgtttag cttgtaaaaa
     1441 accaaaatgt gtgggtggat gtccagtagg aatcgacata ccagggttta taacaaaaat
     1501 taaagaagat gatatagaag gtgctgctaa agtaatagct aagagcagtt cattaccagc
     1561 tgtttgtggt agagtatgcc cacaagaaag tcaatgtgaa ggtgtatgta tacttggaat
     1621 aaaatcagat gctgtatcaa taggtaaatt agaaagattt gtagcagatt ggtcaaaaga
     1681 aaatgacata aatttatctg atacagagcc taagaaaaat caaaaagtag cagtaatagg
     1741 aagtggtcca gcgggtctag cttgtgctgg tgatttagct aaaaaaggct atgatgtaac
     1801 aatctttgaa gcaatgcatg agccaggtgg agttttaact tacggaatac ctgagtttag
     1861 acttccaaaa caagctgttg ttcaacctga aatagacaat ataagaaaat taggtgtaaa
     1921 aatagaaact aatgtaattg ttggtaaaac aattacagta gatgaactta tagaagatga
     1981 aggatttgaa gctatattca taggttcagg agctggtctt ccaatgttta tgaatatacc
     2041 aggggaaaat gcaaatggag tattttctgc aaatgagttt ttaactagag taaacttaat
     2101 gaaggcttat agagatgatt atgatacacc tattagttct ggtaaaaaag ttgctgtagt
     2161 aggtggtgga aatgttgcta tggacgctgc tagaactgca ttaagattag gttcagaatc
     2221 atatatagtt tatagaagaa gtgaaaaaga gcttccagca agagctgaag aagttcatca
     2281 tgcaaaagaa gaaggcatta tatttaatac attaacaaat cctaaggaaa ttttagtaga
     2341 tgaaaatgga tatgttaaag gtatggtttg tataagaatg gaattaggag agccagatga
     2401 ttctggaaga agaagaccta tagaaataga aggttctgaa tttgtacttg atgtagacac
     2461 agttataatg tcacttggaa caagcccaaa tccactaata tcttctacaa caaagagtct
     2521 tgatataaat aagaagagat gcttaataac agatgaaaat ggtcagactt ctaaagaggg
     2581 tgtatttgca ggtggagatg ctgttactgg agcagcaaca gtaatatctg caatgggtgc
     2641 tggtaaaaca gcagcagcat caatagatga atatttaaaa gcaaaagtta atgcttaatt
     2701 tgtaaattaa agagagggtg tctcaaaatg aactttttag ttcatgaggc actctttttg
     2761 tacaaaatca aatatgtttt aatcatttca acaatgaaaa tttcattcct gtagaaagtc
     2821 taacttttag gtcttagtac aatctctatt ttgatgtagc cactttttta gatggtttaa
     2881 taatttatca gttattattg taatatttga tactaagtaa cataactttg tctaactatg
     2941 ataaaatagt tatacaataa aggtcttgta aggagtgaat aatgtgtggc taataaaatc
     3001 agatttgtca aatctaacat taaataatag aaaaataaat tgtgtaatta atggtatttt
     3061 aatatgaaaa aaagagaaaa agtatttgat gggctagttt ttaagcatat attaatctta
     3121 ttaatactat tagttttgat tgatttaaca agtaccatca tatatgcaag taactctatg
     3181 gaaattactt ctagaaatat gattgaggca tcaaaaagag aacttgaaaa ctatttagaa
     3241 gtaaacatat ctttattaaa agctcttagt caagatgata gattttctaa tggtgaaaca
     3301 tcactaattg aaaagggaaa attattaagt ccatatcaaa aggaatataa tctttttatg
     3361 attggtataa cagatacaaa aggaaatgct tcaagtacat atagggaaaa tgttaactca
     3421 ataaaagata agcctagttt tgagaaagca attaaaacaa agcaagtagt agtttcagat
     3481 attgaagtca gtaatgtaac aggtgacaaa gtatttatca tatatgtacc aattgtaaaa
     3541 aataatgaga tgattgggac catttttgct tctttttatt ttcaagatgt aaataattta
     3601 ataagtagaa gcaattttga tgattctatt aaatttctaa tgatagataa agattatact
     3661 attatttctc atccaaacaa aaaatatgta aatgataaaa gtaagatgct tgatttagaa
     3721 gggaatatta taggaaccac taaaagtgaa atcttaaaaa atataaataa aaaatgtcaa
     3781 ggtgattttt tatcttggga taattggaga ttatataatg taaaatatac gaacataaag
     3841 tggactaatt ggactttagt atcaaaatgt aatatattta agaactttaa aaatctaata
     3901 gttaatttta tgataaagtt gtatttttat atagtaatat ttatgatatt gtggaaatta
     3961 agtaatgcta aattaataga acaacttaaa aaattggctt attatgattc tttaagtgga
     4021 ataaaaaata aagaaaagtt tagaaaagat tctatgtata ttttaaagaa ttattatcaa
     4081 gataattttt acttagtaca attggatgtc aataaattta aatatataaa tgagatgttt
     4141 ggatatgctg aagggaataa gatattaatt catatatcac aggttttaaa taataataca
     4201 aataaatatg aaatttgtgc taggatggat aatgaccatt ttatattgtt gattgcttgt
     4261 aacactgaag atgaactttt aaatagatta tctaaaataa ataaagaaat ttgtaactta
     4321 aatactacta actcatctaa gtataaaatt gttatgtcaa gtggaatata taagattact
     4381 aaaaaggatg atattaaaaa gattgacttg ttaattgata gagcaaatat tgcagcaaaa
     4441 agtaaaaagg aaaaatatga acattcatat tcatttttta atgaagatac aagaaatcga
     4501 ttgtacaagg aaaaacgatt ggaagataat atgaataaag ctttagaaaa aggtgaattt
     4561 atagtatatt atcaacctaa gtatagttta gatgatgtaa atgaaattga aggagcagaa
     4621 gccttgataa gatggaatag tccagaattt gggtttattt cacctataga ttttgtacct
     4681 ttatttgaaa agaatggttt tattgtaaat attgatatgt tcgtgtttga agaagtatgc
     4741 aaaacattaa acaagtggat aaataaaggg tatactcctg taccaatctc tgtaaatatg
     4801 tctagagttc atttatatag ggataatttt atagaaaata taactgattt gataagtaaa
     4861 tacaatatat ctcctgagtt tattgaactt gaactaacag aaagtgttgt gtttgataat
     4921 ttaaatatat tgattgatat aatgaaaaag attaaagaga taggattcct tatatcaatg
     4981 gatgattttg gttcgggata ttcatcttta aatctgttaa aagacctttc ttttgatatt
     5041 ttaaaattag atagaggatt tttaatcgaa acaacagata caaaacgtgg aaaaattatt
     5101 atttctaaga ttgtggaaat ggcaaaagcg attgatatta aagtaatctg tgagggcgta
     5161 gaaacttatg aacaagtaga gttcttgaaa gagataggtt gtgataaggt tcaaggatac
     5221 ttatttgcta aacctatggt attagatgaa tttgaaaaac acttaaactt taaatttgat
     5281 taatcattaa taattattag actataatat accctaacaa cttacttaca aatatgtaag
     5341 ttaattgtta gggtattttt atggttaaag tagtatattc acgatattat ggtattgtag
     5401 taaaaataaa aaattatgga gaatataaaa tgaacatatt gagtattaga aatatatcaa
     5461 aaacatattg cggaaatatt ccatttaaag cattagataa ggttagtcta aatatagaaa
     5521 agggagaatt tgtgtctgtt atgggaccat ctggaagtgg taaatctact cttttaaata
     5581 taatatctac agttgataga cagagtgaag gtgaggttgt attagatgga tatgatgtaa
     5641 gtaaattgaa aggagaaaaa cttgctgaat ttagaaggaa acaacttggt tttgtatttc
     5701 aggattttaa tttgattgac actttaactg ttggcgaaaa tattatgttg ccacttacac
     5761 tagagggggc aagtatcaaa gatatgaata tacaaactaa aagtatatct aaatttttag
     5821 gaatagataa aattttagat agaaaaacct atgaaatttc aggaggtcaa gctcaaaggt
     5881 gtgctattgc tagagctatt ataaataaac ctgccatact tcttgcagat gagccaactg
     5941 gaaatcttga ttcaaaatct actgatgatg ttttgaaatt atttactagg ataaacaaag
     6001 aacaaaaggt aacaacactt atggtaactc atgaagcata tagtgcaagt tattcagata
     6061 gagtaatttt tataaaagat ggttgtatat atacagaaat aaaaaaatct gaaagcagta
     6121 attctttcta ttctgatata ctagctgttt tgtctcaaat aggaggtgta agatagtgga
     6181 ttttagaaag tttgcttata ataatatagt taaaaatttc agggcatatt tagcatattt
     6241 tttaagttct gttttttcta taatgatatt ttttgttttt gcagtgtcta tgttccatcc
     6301 aattataaca aaatctggaa tgcagagtgg ttctactgca tttatggctc ttatgacttc
     6361 agaattaata atattcggtt ttagtctact ttttatattg tactctttag gaaatttctt
     6421 gaaatataga ttgaaagatt ttggagtatt gatgataatt ggaatgagta acagacaact
     6481 gaaaaaattg atattaattg aaaatttgat aataggattt ttagcagtga tgttaggaat
     6541 attacttggt ttatcaattt caaaattgtt tttattgtat ttaagcaaac tgttttatat
     6601 gaatttaact gaattttatt ttccagtaaa agcaatgcta ttgactattg tatcatttat
     6661 gattttattt ttgataacag gaccaatgag cttaaaatta ttgagtaaaa agagcatttt
     6721 agagctttta gtaggtacaa aaaagcctaa aaaagagata aaatcttcaa agctatttgg
     6781 aataattggt gtaatatgtc ttatattagg atatgctatg atactatttg gcaataagtt
     6841 aaaaataaat ggaggtatgt atacaacagc actattaatt actttgggga tatttttatt
     6901 tttttctcaa tttactattt tgatattaaa gtatttaaaa aataaagaaa aattttataa
     6961 gaacaaagta aatatgttac ttataagtaa tttgatatat aaattaaagg ataatacacg
     7021 attgttattt ttaataacaa tacttttatc ggctacttta gtagcattta caacaacttc
     7081 aacacttgta agttctcaag gagaagcttc taaaaataat tttccaatgg tttactcata
     7141 tttttcagag cataataatg aaaaagaata tgaagaacta caacaaatta gagaagcaat
     7201 caaaggatat gattataagg agttaagttt tcctgtactt aaatataata gggaagtctt
     7261 actaagtgta aagaattata acaaattatc aaaggaattg ggattagata aaattagttc
     7321 aaagttagaa aaatatgaag gggttataat tcctagtagc aattcacaag aacatataaa
     7381 ttctttaaaa caattaaaaa attatagtat aaaaaattta aatatagaag taaaaaaatc
     7441 aaaaaataaa ataatttttc ctacaggact gtttagtaaa gttgtagtta taagtgatga
     7501 cttatacaat gaagtaaaag gtgaattttc aaaaattaat tttcatggat ttgattataa
     7561 aaactggcaa agcagtgcac agataactga aaatttaaaa gaaaaacatt ttaatttgga
     7621 tagagaatat aacaggtcat attttttaac attaccagat atgtatttag tagagttaca
     7681 acaaagtaaa attcttcaat ttatgggtgt ttttatagga gtgatattat ttatagcagt
     7741 ttttagtttt ttatacttta gattatatac agacgagata gaagataatg taaagtacaa
     7801 aaaactatca aaaataggtc tatcttttaa aaatatgaat aagattgtta gtatagagat
     7861 aggtacattg ttttttatac cgtattttat agcaattata aatagcataa tttctttact
     7921 gtttttaaat caagtattaa ataatagttt taagttagaa aatttgggta tattactact
     7981 gatatcatct atatattcac tttattttta cttattaaaa aaaatttata ctagaaaagt
     8041 ttggtttttt gattaaagtg gtatataata gatatataaa ttaatattta aaaagtagtt
     8101 aatattaggg gagggcattt gcacctccct tgtaatatgt ggaaaattga atattagaag
     8161 aaggaggaga cttgatggaa aatcaacaat tattaggaac agaacgaatt agtaaattac
     8221 tactaaaata ttctatacca gctataatag gtatgttggt aaatagttta tataatgtag
     8281 tagatagaat ttttattgga aatataccag gggttggacc acttgcaata acaggtcttg
     8341 gtgtaacaat gccaataatg acaataatac ttgcatttgg gatgttgata ggtataggaa
     8401 ctactactac catatcaata aaacttgggc aaggtaaagt agaagaggca agaaaactta
     8461 taggaaatgc aatgacgtta tctgtaataa caggaattat cataatgata cttggaatat
     8521 tatttgcaaa taaaatactt acattgtttg gagcaagtga aaatacttta atatatgcaa
     8581 aatcatatat caatattata ttattaggta cagtagtaaa tcttctttcc ttttctttaa
     8641 accactcaat aagagctgat ggtagtccta aaatttcagc aggaataatg atagtaggat
     8701 gtttaactaa tatagtttta gactggatat taatatttgg attcaatttg ggaatacaag
     8761 gagctgcaat tgctacagta acttctcagg ctcttacagc tattttaact atagggtatt
     8821 atataagtgg aaaatcaaac ttacgattca gtaaatctaa tctaaaatta gataaaaagc
     8881 taataaaagc agtgtttgca ataggtatgt cgccatttgc tatgcaactt gcagcaagtt
     8941 tagttcaagt aatatcaaat attgcactta aaactcatgg tggagattta gctatagggg
     9001 ctatggctac tatttcttct attgcaatgg tatttttgat gcctatcttt ggaataaatc
     9061 aaggagcgca acctattata ggtttcaatt atggagctga aaaatatgat agggttaaaa
     9121 aagcatattt aggttcatta gtagtagcta ctataatttt atgtatgggt atggtagtag
     9181 ttatgctttt cccagaagct attataggta tatttaataa agaccctgaa cttatgaata
     9241 tatctgttaa tggattaaga atatacttac ttatgttgcc aattgtaggc ttatcagtta
     9301 caggaacaaa ctttatacaa tctataggaa aagcaaaaat ggctatgtta ttgagccttt
     9361 taagacaagt aattttattg ataccagcag tattaattct tcctacattc ttaggtttac
     9421 agggtgtatg gacagctcaa ccagtgtctg attttatagc tacagtaatt acaggtatag
     9481 ttgtatttag agaattaaaa agatatactc ctaaaactga aaaattaaat gaaaatgaaa
     9541 gactaaacga gataactact gagtaaaaat ataaattcag ataattgagc aagaaattgc
     9601 aaaaaatacg attatatgat aaaataaaac cgctgtcaag gataagagaa attctaatta
     9661 tggagtgatt ttatggaatt tacaaagaaa agcaaattaa aaaatatgta tgtaaacatg
     9721 aatgtagcta gaatgttgtc taagataaat gagtataagg gtagacaact gctttacaaa
     9781 aaacaaccaa aagaaatttt agaaaactta gaaaaaaaat ctttagtaga ttgttctgaa
     9841 tctacataca ttggaaatca aagagatagt agtaatttta atctagaaaa gttgataagt
     9901 aatgaagtta cacctagaag tagagaggaa ttgagtattg ttgaatacag agatgtggta
     9961 aaaactatta acagtgctta tgagagtatt cccatcagtt cacaaacgat tcttgagctt
    10021 catggatact tatataaatt ttcatctact agaggaggaa gttataagtc agataatgat
    10081 tttattgaac acaatttaaa acctagcgaa tttataagta ttgattcaag tgtcaataaa
    10141 gttgaaaaag ctgtagaaga aatatgtgaa gcttataata cattgataga ggaagatgaa
    10201 attgatatat taatattaat ttctgcattt gtgttagatt tcatattgat acatccattc
    10261 aaagaaggta atataaaaat ggcgagagta cttatcctat tgttactaaa taaaaatggg
    10321 tatgaagttg gaagatatat aagtttagga aagatatttg atgatagttc atatgaatat
    10381 tatagtaatt taaattattt gaaagcctca ataggtagtg aaaaagcaga tatgaatgca
    10441 tggattgagt attttttaga gacgatatta actgcatatg agaagctgga tgatagttta
    10501 aatatatctg ataagaaaag acaaacaaaa actagtagaa tagagaaaat aataaattct
    10561 acccttggat attttacaaa agaagatata agagatttat gtcctgacat tccagaacct
    10621 actataaata gagtttttaa taatctaaga aaacaagata agatagaagt tgttgcaaga
    10681 ggaagaagtg caaaatggaa gaaaaaatat taaaaaatgt ttagataact aaaatggtat
    10741 agtgttttac actataccat ttttaatgtc attagcatga gcaataattt aacaacttaa
    10801 gcaatatcaa ttttatttat tgattatttt tgaactgaaa aatggcgtta tatggtatac
    10861 ctcagtggta attaattttg gatgagaaga tataatatta cagaatatat taggagtata
    10921 tgtagaaaaa atataataaa agtgtgaggc ttagtaatta acaaattatg tggcttcaca
    10981 cttttttata aaataaagat acttagcaga agaatatttt agttgatatg tttataagat
    11041 ggtacttgtt tgggtaaaag tataaatagt aaaaaagtag aagtaatgtg tataaaaacg
    11101 taagttacat tctgtaataa tagttatttt ataattcaac aaaagaatag aatgggtata
    11161 tttgtgaaat aataatataa aataatctta ggagtgagct tatgaagaaa aaattattat
    11221 atataaatgt aaattcaaaa cctgaagact tatcttcaag taaaactgta gcaagaaaat
    11281 ttataaataa atttatggaa aaaaataaag attttgaggt tgaagagata gacctttata
    11341 aagaacatat accaagactt gagtatcagt actttgaaaa aagaaatagc atagttagtg
    11401 aagaaaatgc taaaaattta gacgataagg atagaaaaga acttacaaag attagaaatc
    11461 tttgtgacca atttgtgagt gcaagtgtat atgtaatagc agcacctatg tggagtttat
    11521 cttttccagc accacttaaa gagtatatag attgtataat tcaagatgga aaaactatat
    11581 cttttgaagg caatgataaa ccacaaggaa ttttaaatga tattgataga agtatggtat
    11641 atatacaatc ttctggtgga cacataccat gggtgttgaa gccagttatg aataaagggt
    11701 taaattatgt ggaaagtatt atgaaattta taggaattaa aaaatttgat gaattgttag
    11761 tagatggaac tggaactagt gaagaagaaa gacaagctgc tatagaaaaa gcagctgaaa
    11821 aaatagatgg tataatagat gggatgaaat tttaattata atattttatt aaaaatataa
    11881 aggaaaaacc tcttttaatg tagaaactta tattgaagga ggcgagaatc atgcgagaag
    11941 aaaaaagtaa tgaaaagtat gattgttatt ggtgtaatca agagaataat ttctgtgtag
    12001 aaataaaaga taatatagtc atgatagatg atggcactgg tacgttaaaa caagccgttt
    12061 tcatagggta taaacaaatc caaattaatt taaactgttc acattgtcaa aacttgaata
    12121 gaataaaatt aaatttgtag aattttaaaa tggaaattat tgaaggaatt aaaaatagaa
    12181 agtgttaata tttagactga aaattatatt ttatacaata aaagtttatt taaataacat
    12241 tgtattttaa tatttatttt cagtcttact ttattaaaat tattttttgg ttatgtctgg
    12301 aaaatatctg tctactaatt tttctgaaat attttttaat tggtcagcca ttgaagtcag
    12361 acttaataag gctatcaata aatcatcttt attttctgga tttatactaa tattttcaaa
    12421 attagactct ataaagtccg gtatattctt tctttgattt tcttttatta acatgaattt
    12481 ttcgtaacat gaaactaaat ctttttcact gaaattatcc ttttcataca caccatgtaa
    12541 gattctttta atttcttgta ttgtaagtgt atttttcata gaataaataa ttatcatctg
    12601 taaaatttgt tctttagtat acttttttcc cttaactggt ttgattaatc cctctttgct
    12661 gtaattattt atcatggttt tagtaagaat tttatcactt tcaaatcttt tattggcaga
    12721 aagcttattg tcaattagag aaataatttg atccatatat aagtcaatag atggaatatc
    12781 gtttgaagtt atatctgcat tatttagtgt ttctagtatt atagtattaa gttcattatt
    12841 catcatatgt tacctccata atatatataa tacactaaat tagaaagtaa aacaatatta
    12901 aggcttgtaa aaaaaacata aagtggtata attaaatata taatatagta atggaaacta
    12961 tatttgtgaa tctgattttt aaaatacata attgtaggag gacgaaatgt atcaattttt
    13021 tttaaagggt agagacccaa taagcagttt aactcatttt ataggtgctt gtttatctct
    13081 tttagcaact ataattttag tttttcaatc tgttacatta caggaaactt ctctcttgat
    13141 gatagtatct gtatcggtat ttggattttc actaattgct ttatatagcg caagttcgta
    13201 ttatcatttt ttaaagggga caccagaaca agaattgttt tttagaaaag tagaccatgc
    13261 aatgatttac gtattgattg ctggctctta tacaccaata tgtctgaatt ttatggaaaa
    13321 aaaagaaggc ataatatttg ttacagcaat atggatagtt gcatttattg gtataataat
    13381 aaaaatattt tggatggatg cacctagatg gttatctacc agtatatatt tacttatggg
    13441 gtgggcaatt gtttttgata taaatgcatt caattcaatt cctaaggatt gccttagact
    13501 attaattatg gaaggtgtat cttactctat aggtgcaatt atttatataa ttaaaaagcc
    13561 taatataagt cctgagtttg gatttcatga aatatttcat atttttatta tgataggttc
    13621 tttatttcac tttttagcag ttttattgta tgttttatag ttttaaaaga tttgattaat
    13681 tgatactaaa attctacaat gaataaaatg gaactgcctt atatagatta tatcttcgac
    13741 aataagacag ttccttatta taaatattaa attctactta taataaaatt ttgataaaaa
    13801 tagtccattc aaaatgccta ttccataatt cctttaatgt acttttcatt tacgacatat
    13861 agtttgaatt tatgtactat aattatatcc tctaaattct ctgaaacact aacaattctg
    13921 acacatttgt ttctaatttc tacaggtaaa tctaaatcat ttgttaaatt cacatagtca
    13981 tcatatttat acataaaatc atccctttac attaagatat acttattata ctatatttta
    14041 ataagttttt ttaaattatt acaatttttc ccgaaatttc attttttata cataaaattt
    14101 ccaaattagg taaagattaa attttttatt gtataaacaa aagttacata taaaatattt
    14161 tattcaattt tttaaataac tattgccaat ttttgaaaaa tatattatta ttaatagtaa
    14221 caaaaaactg taaatttaga atattaataa aaattatttg ggaggaaatt atcatggtga
    14281 gattaaatat agtaaaatta aatacaaaat taaatcatca ccatcatcat agattaaagg
    14341 gattgtaata tgaaattaat tcatagacac aagccctgtt tttagtgtaa ttggtcttgt
    14401 atctatgatg ggaagtaatt ttaagagaaa attatagcca tataggtata aagcctatgt
    14461 ggctttttta ttgttttaaa tttgcaaata taagagtaaa tttaatgaag agggtgagaa
    14521 tgtgaaattt ttgaggattg aagatgaggt tatttattct aaaaaaagat ataaattaaa
    14581 aagggaaata gataaacttt ttttggatga agaatatttt caaatagaac cacaattatt
    14641 tgaagaatat gatgaattta caactataaa taataaaatt ccagaagaat ctatggtaaa
    14701 ggttgtaaat ggaaaagtaa tggtacttag agctgatata actacaagta taataaaaag
    14761 ccttgtacca agatgggaag atggtttaaa gcttaagctt ttttataatt catcaatata
    14821 taaaaataaa aatacagttg gaataaaaga gataagacaa ataggttgtg agtatctagg
    14881 agaagcctct gtagaagcag atagagaagt agtgaaatta gctcttaaga tacttgaaaa
    14941 atacaataat aattttattc tagaggttgg gagtagcaag tatattcatg gtttgttaga
    15001 agaattaaat ttaaataaga attgtgaaaa tcaaattaaa aatttactat atacgaagaa
    15061 cactcatgaa ctaaaagtat atattgaaga attaaaaatt aagcgtgaag taaaagagtt
    15121 attatcaaat attttaagtt tacaagggaa tttatataat gttattgaaa aatcaaacaa
    15181 gttctattgt aataataaga tgaagcaagc tcttgaagaa ttaaagcaag taaataattt
    15241 aattgaggaa tgtaattttt tagataaggc tagatttgat ttatcaatga ttactatgct
    15301 tgactattat gaaggaatta tgtttagagg atactaccca aactcatata aagaaattct
    15361 tagtggcgga agatatgatt ctttaactaa agagtatgga aaagaaattc cagccattgg
    15421 atttacacta agtgttgatg agttaatgaa gtatgtatat aagtaaagtt tagaaggagg
    15481 gattgctttg gattatttga ctatagcact agcaaaagga cgaattgaag gggaatcatt
    15541 taaaaaattt aaaaaaatgg gtctaggaga cagtatagac acagatacga gaaaactaat
    15601 ttttaaagat gaagaaaata aaattatata tatacatgta aagccatcag atgtagttac
    15661 atatgttgaa aaaggagtcg ctgacctcgg tatagcaggt aaagatacta ttttagaaaa
    15721 tgagacagat gtatatgaaa tatatgattt aggttttgga aaatgtaaat ttgctgtagc
    15781 aggattaaag ggagatagca tatatagaga agatgaatat ttaaaagtgg caacaaagta
    15841 tcccaatata gcaaaaaagt attttaaaga gaaaggtcaa aaaattgaaa taataaaatt
    15901 aaatggttct gtagaactag ctccaatagt agggttgtca gatgtaattg tagatatagt
    15961 tgagactggc aatacactta aagcaaatgg tcttgaaata ttagaagaca tatgtaatat
    16021 aagtgctaga attatatcaa atagagctag ctatagattt aaatatgaac aaattcagaa
    16081 tataattcgt ttatttgaag aacttgataa ttaaaatata attgagcttt ataaggaggg
    16141 atttaagttg agagaaaaag aaagcataag agagttaagg ggatatgaac caaatcacgt
    16201 aaactgtaaa gtaaaacttg atgcaaatga aggaagtaag aggttgttta aataccttat
    16261 taaagaaatt tcagatagtg atatagattt aaatttatat cctgaagatt cttatagtga
    16321 tttgaaagaa tctataatag attacataaa tatttctgga gtaaataaga aaaatcttct
    16381 agtaggaaac ggttcaagtg aaataattga tttaataata catacttttg tagataaaga
    16441 tgaagtaata ttgagtttct ctccaagttt tagtatgtat tctatatata gtcagataaa
    16501 tgggtctaaa tttataggtg ttgaaagtga tgaaaattta gttataaata tagataatgt
    16561 gattgaaaaa gtaaaggaga ataatcctaa aatagtaatt gtatgtaatc ctaataatcc
    16621 aactggaaca atattaaaga gggaagagat aataaagctt ttagattcaa ctaatagtct
    16681 agtggtttta gatgaagcat atatggattt tggagaagaa agcatgttaa gtgatgtttt
    16741 taagtatgat aatctaatag ttcttagaac tttatcaaag gcatttggat tggctggaat
    16801 aagaacagga tatatgttat ctaatagtag tttaataaat tctgttgaga aagtaagacc
    16861 tccatataat ttaaattcat tatcagactt tattgctaca agagcactta gaaataaaga
    16921 tgtggtaaag gcttatataa aggaagtaaa agaggaaaga gaagttttat atgaagaaat
    16981 gattggtatg ggaattaaag catacaaatc acaagcaaat tttatattat tttattctga
    17041 aatcgaaaat ttatcacaaa aacttattga tagaggtgtt ttaataagaa aatttggtgg
    17101 aaagttagaa aattattaca gagttacaat tggtgataaa gaagaaaatt ctatgtttgt
    17161 tggagctata agagatattt tgaaaaagga gaaataggta tgagaatttg gaaggtagaa
    17221 agaaatactc ttgaaacaca gattttagta gaattaaata ttgatggttc tggtaaagct
    17281 gaaatagata ctggaatagg atttttagac catatgctta ccttaatgtc atttcatggc
    17341 aagttcgatt taaaagtgat ttgtaagggt gatacatatg tagatgacca ccatagtgta
    17401 gaagatatag gtatagctat aggtgaagca tttaaaaatg cactaggtga caaaaaagga
    17461 atacgaagat attctaatat ctatatacct atggatgaat ctctatcaat ggtagcaata
    17521 gatataagca atagacctta tcttgtattt aatgctaaat ttgataccca aatgattggt
    17581 agtatgagta ctcaatgctt taaagaattt tttagagctt ttgtaaatga atcgagagta
    17641 acattacata tcaatctctt atatggggaa aatgaccatc ataagataga aagtattttt
    17701 aaagcatttg caagagcttt aaaagagggt agtgaaatag tttctaatga gatagcatct
    17761 tcaaaggggg ttttgtagaa tgaatataat agtagactat ggtcttggaa atatagattc
    17821 tgtgtctagg ggttttagaa aagctggaat agaaactaaa atttctagtg atatagatga
    17881 aataaaacag gcagattcac ttatacttcc aggtgttgga gcttttagag attctataag
    17941 tgccttagat aaactaggat tgataccaat aataaaggag catgtttcta agggtaaatt
    18001 tatgataggt atatgtctag gaatgcaatt actttatgaa aaaagttatg agtatggaga
    18061 atatgaagga ttaggactta taaaaggaag tattgataaa ctggatataa gtttaaaagt
    18121 gccacatatg ggatggaata atctaaaatt taataaggca aatgatgata tacttaaata
    18181 tataaatgaa gatgattatg tatacttcgt tcactcatat tatgcaaatt catcaaatga
    18241 agaactaatt gcattttcag aatatgaaaa gaaaatccct gctattgtta gaaaaggcaa
    18301 tgtatatggt atacaatttc atcctgaaaa aagtggagaa gtagggttaa atatattaag
    18361 agcatatggg gagatgataa aatgataata tttccagcaa tagatataaa agataataag
    18421 tgtgtaagac tcacacaagg agaatttgac aaagtaaatg tatattatga taatccatta
    18481 gaagtagcat ataaatggaa aaatgaagga gcagaatata tacatatagt agatttaaat
    18541 ggtgctagaa gtgaatttgg tgtaaataca aaaataattg aggacatagc aaacaatata
    18601 gatataccta ttcaagttgg cggtggagtt agggataagg aaaaagtcaa aagtttaata
    18661 aatgctggtg ttactagagt aatattaggt agtatagcga ttgaaaattt gaatttagtt
    18721 gaagaattgg tcaatgaata taaagagaaa attgtagttt ctatagatgc taaagatgga
    18781 aaagtagcag ttaggggatg ggaagttgta agcaatgtag actcattaac attatgtaaa
    18841 caacttgaaa agataggtgt acaaactatt gtttatacag atatatcaaa agatgggatg
    18901 ttgcaaggtc caaattttga catatatgaa agaatagcaa aggaaacatc actaaatgtt
    18961 attgcttctg gaggcgtaac ttctatagaa gacgtaaaaa gactaaaagc tatgaactta
    19021 tatggtgcaa taataggtaa agcgctttat gataaaaaga tagactttaa ggaggcacaa
    19081 caattatgct tactaggaga ataatacctt gtctagatgt gagaaatgga agagttgtaa
    19141 aggggaaaaa atttaaagat atagtagatg ttgatagtcc agaagtactt ggaaaatttt
    19201 atagtgattg tggtgcagat gaacttgtat tctatgatat aactgcatca aatgaagaga
    19261 gaaagacatc tttagaattt gtgacaaaag ttgcagaaaa tataaatata ccattttgtg
    19321 ttggtggagg agtaaataaa ttagaagatt tcactgatat tttaagaaaa ggtgctgata
    19381 aagtttctat aaattcatca gcagtcaaaa atcctgaatt aataagggaa gcttcattaa
    19441 aatttggagc acaatgtgtt gttttatcga tagatgcaaa gaaaaatgaa gagggttcat
    19501 ggagcgtata cgtaaaaggt ggaagagaaa aaactaatct ggatgcaatt gaatgggctg
    19561 ttaaaggtgt agaacttgga gcaggagaaa tcgttgtaaa tagtatggat gaagatggaa
    19621 tgaaaaatgg atatgatata gaattattat caaagataac ttctttagta aatgtacctg
    19681 ttatagcatc tggaggcgct ggaaaaaagg aagattttta tgaggcggtt aataaatcca
    19741 atgtagatgg aattttagca gcatctgttt ttcattttgg cgagataaaa ataaacgatt
    19801 taaagaaata tttaaaagac atgggtgtag aagtaagatt ataagttaaa tactaaaagg
    19861 agattagaaa tatggataat aagtgtaaca atgtatacag tgatgaagta gagaaattta
    19921 taagaagtat aaagtttgat gataaagggt tagtccctgt tgttgttcaa gaggtagtta
    19981 gcaaagatgt actgatgtta gcatatatga ataaagatgt gataaagaaa accttaaaag
    20041 ataaagtagc ttgctatttt agtagaagta gacaagaact ttgggttaaa ggtgagacat
    20101 ctggtaatac tcaaaaagta gttaagatgt cttatgattg tgatgtagat actatactac
    20161 tttttgttga acaaactggt gtagcttgtc atacaggcaa ttattcttgt ttttacagag
    20221 acttgtttga tgatagttca aaaatggagc ttgaagtaca aacaaatata ttgaaagaat
    20281 tatatgattt aatcaatgaa agaaaaaata atccagttga aggttcttat actaattatc
    20341 tttttgaaaa aggaatagat aagattctta aaaaagtagg tgaagagagt agtgaggtaa
    20401 ttatagcatc aaaaaatact gataaaagtg aacttattta cgaaattagc gatttagtat
    20461 atcatacatt ggttttaatg atagaaaaag gtgttgaaat tgatgaaata aaaaaagaat
    20521 tacttaaaag aagaaaatag aataagatag attaatgggt aataagaaaa tataccactt
    20581 ttaataaaaa gtggtatatt ttttatttta tattttgatt tatagaataa tatttaaatt
    20641 acttgataaa attaactata tatataattt attgataaaa taattctagt acattattta
    20701 ttttaga
//
LOCUS       DAYAPA010000067        20444 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.067, whole genome shotgun sequence.
ACCESSION   DAYAPA010000067 DAYAPA010000000
VERSION     DAYAPA010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20444
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.067"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            331..1524
                     /gene="nifV"
                     /locus_tag="ACGI59_002269"
     CDS             331..1524
                     /gene="nifV"
                     /locus_tag="ACGI59_002269"
                     /EC_number="2.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004410 - homocitrate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homocitrate synthase"
                     /protein_id="HFG9740055.1"
                     /translation="MCVISKDRAKEIKIVDTTLRDGEQTAGVVFANREKIMIAEMLSD
                     LGVDQIEVGIPTMGGDEKNVIKHICSRNLKSDIMAWNRAVIKDVEESISCGVDAVAIS
                     ISVSDIHIENKLRTSRGWVLENMAKTVEFAKKNGLYVSVNGEDASRADIDFLTEFINV
                     GKQAGADRFRYCDTVGVMNPFSIKNAIETLYERTNFDIEMHTHNDFGMATANALAGIA
                     AGANYVGVTVNGLGERAGNAALEEVLMALKCVYKCDLNNIDTRKFRGICEYVAQASGR
                     ILPTWKPVVGDNMFIHESGIHADGALKDPHNYEPFDPSEVNLERKIVIGKHSGRAAVV
                     NKLSEYEMYISPENATKLLNAIRATSIRLKRSLMDKEILQLYCDILAHEKGTTEEEAV
                     RGSYI"
     gene            1549..3474
                     /locus_tag="ACGI59_002270"
     CDS             1549..3474
                     /locus_tag="ACGI59_002270"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HFG9740056.1"
                     /translation="MGDNIVYKIIKKHIVDGEAVAGSSIGIKIDQTLTQDSTGTMTYL
                     QLEAMGIDKVKTKRSVAFVDHNMLQQGFENADDHKYIQTVADKYGVYFSKPGNGICHQ
                     VFLERFSTPGDTLLGSDSHTPTAGGVGMMAIGAGGLDVALAMAGGAYYIKAPKVCKVN
                     LVGKLNNMVSSKDIILEVLRKQTVKGGVGKVYEYGGEGVKSLSVPQRATITNMGAELG
                     ATTSIFPSDEKTLEFFKSQGREDAWVELKPDADAVYDEEITINLDELKPLAAKPHSPD
                     NVDEVENIGKIKIDQVAIGSCTNSSYEDLMKVAQILKGNKVHKDVSLVIAPGSRQVME
                     MIARNGALADIISAGARILENSCGPCIGMGQSPGTDSVSLRTFNRNFYGRSGTLSAQV
                     YLVSPEVAAVSAIKGVLTDPREFDIKFTNLDVNEFLIDDSMIIKPADVGSDVEVVRGP
                     NIKPFPLNTELSQSIGGKVILKTEDNITTDHIMPSNAKLLPFRSNIPYLANYCFNTVD
                     TEFPQRAKDNNGGFIVGGDNYGQGSSREHAALAPLYLGVKGVIVKSFARIHKANLINS
                     GIIPMEFCDEKDYENISLLDNLEIPNILDNLGSGILEVKNTTKGTSFKVKVELSAKEV
                     DVLKAGGKLNYTKNQAN"
     gene            3496..4491
                     /locus_tag="ACGI59_002271"
     CDS             3496..4491
                     /locus_tag="ACGI59_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HFG9740057.1"
                     /translation="MYKVTLIPGDGIGPEVAKAMKKVVEATGVEIEWEEVNAGEAVIE
                     EYGTPLPEYIIDSIKKNKIAIKGPITTPVGKGFRSVNVTLRQALDLYVNLRPIKSFKG
                     IKSRYEDVDLVVVRENTEDLYAGIEHKIGDYAAESIKIITRSASERIVDFACNYVKDN
                     KRKKVTAIHKANIMKMSDGLFLDVFREVASKHGVEYDDLIVDAAAMNLVLNPENYDVM
                     VMPNLYGDILSDLGAGLVGGLGIIPSANIGKDCAIFEAVHGSAPQIAGQNKANPTALI
                     QSSVMMLRYLGEYENAQKIETALEKVFLEGSKLTVDLGGSASTTEFADEVCKYIV"
     gene            4816..5289
                     /locus_tag="ACGI59_002272"
     CDS             4816..5289
                     /locus_tag="ACGI59_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFG9740058.1"
                     /translation="MVVSVEVCLIKTLDSNILREVGTLSRAVNSINDIKYKELKLQKG
                     QFTFLTRICENPGINLVELSNMLKVDKATTTKAIQKLIKAGYVDKKQDKFDKRGYNLT
                     PTDKSLEVYELIIEEENRSIEICFDNFTDEEKQVVTKLLEKMSKNVENEWFKVKR"
     gene            5323..5817
                     /locus_tag="ACGI59_002273"
     CDS             5323..5817
                     /locus_tag="ACGI59_002273"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFG9740059.1"
                     /translation="MIRKANMNDLESIMKIIKSTVEEMKTYNNTQWDENYPLEKDFVS
                     DIKKQDLYIYEVDGEVAGFICLNYEEPEEYLGLNWSSNKKAMVIHRMAVNPNFRKTGI
                     ASKLVDFAEKLAVENNVSYLKSDTYSINSKMNLLLTKCGFIKIGEMSFLGKEKSFYCY
                     DKIL"
     gene            5914..6435
                     /locus_tag="ACGI59_002274"
     CDS             5914..6435
                     /locus_tag="ACGI59_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFG9740060.1"
                     /translation="MIGFLKKRRSIRKYKDVEVEKEKLDKILKAALLAPSSKGLRTWE
                     FIVVDDKEKLINLSQCRTKGGGFFLKNAPLAIVIIADKEKNDVWIEDASIAASYIQLQ
                     AHELGLGSCWIQVRNRMHDDNIEADKYIREELKVPSKYSVECIISIGYPDEEKKAYGD
                     SDLDYKKVHFNNF"
     gene            6624..6935
                     /locus_tag="ACGI59_002275"
     CDS             6624..6935
                     /locus_tag="ACGI59_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFG9740061.1"
                     /translation="MGIFKTKMDEDWKVNYIKEFNEMRDSYESKLQKKQFEVDSLKSE
                     LDRLRSYKNSLKPKEKQITDDDINNIKSLRRDGLSYKEISNQTSWSKATVSRVLNGLY
                     D"
     gene            6963..7649
                     /locus_tag="ACGI59_002276"
     CDS             6963..7649
                     /locus_tag="ACGI59_002276"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HFG9740062.1"
                     /translation="MKYSYKKCIIDSILLMFIVQILRMILNYVLLSQFEFTLENFNII
                     NLISFTLVGLSLILFLKDNSLYNKVRNRKITEAFEENKNNILIEKCKLILFVVVLSLA
                     IIVTYCTKGYVLFNVTMMTLSVLIVPIFEELFFREYIWNYLSNFIKSKSKIICITSIL
                     SGIYNIGYIDVIRNYVILYNNSSYTFEVIISKIMIGTVFGIVLGLVKYRFRDVGFCIL
                     LRSLFAIFIR"
     gene            7881..8765
                     /locus_tag="ACGI59_002277"
     CDS             7881..8765
                     /locus_tag="ACGI59_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFG9740063.1"
                     /translation="MKFVTFCSSNEEKIGVFNSETNSIYEINSLGLSKLYTDMNDFIE
                     NVSTGDLEKIKNNSFENAKCYKLEEVKLCSPIVRPKKDIICLGLNYKDHVNEIPDGVI
                     KNVVMPDYPIYFSKRADKIIGVDDKISLHGDLVEKLDYESELAVIIGKEGINISKEDA
                     YEYIFGYTIVNDISERALQDKHVQWFRGKSLDTHTSMGPCIVHKEEFEHPLKLDISSV
                     VNGEVRQDSNTEYFIFDIPTIINDLSRGMTLKPGDIISTGTPAGVAMGMNPQVYLKHG
                     DIVECKVEGIGVLKNIVD"
     gene            8864..10039
                     /locus_tag="ACGI59_002278"
     CDS             8864..10039
                     /locus_tag="ACGI59_002278"
                     /EC_number="2.1.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolecarboxamide
                     formyltransferase"
                     /protein_id="HFG9740064.1"
                     /translation="MARELELKYGCNPNQKPSKIYMKNGELPIEVLNGKPGYINFLDA
                     FNSWQLVKELKEATGLPAATSFKHVSPAGAAVGVPLSDTLKQIYFVDDLELSPLACAY
                     AMARGADRMSSYGDFIALSDVCDKETATIIAREVSDGIIAPGYTDEALEILKGKRKGN
                     YNIVKIDENYTPEPIETKDVYGITFEQGRNEILINEDLLKDIPTDNKIFTDSAKRDLI
                     IALITLKYTQSNSVCYAKDGQVIGVGAGQQSRIHCTRLAGNKADTWYLRQHPKVLNLK
                     FKKDIGRPDRDNTIDVYLSDDYMDVLADGIWQNFFEEKPEPLTGEGKRAWLKTLTGVA
                     LGSDAFFPFGDNIERAKRSGVSFIAQPGGSIRDDNVILTCNKYNIVMAFTKNRLFHH"
     gene            10167..11021
                     /locus_tag="ACGI59_002279"
     CDS             10167..11021
                     /locus_tag="ACGI59_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9740065.1"
                     /translation="MALNIRNYSLLCNQGTAKFNEDVVGINPFGAWVLDGATGLNGKN
                     LVSTESDARWYVQWWNRYLYKNINKEESLKSIINTGIIEVKKEYELRLNGFKVEKLDL
                     PSSSIVVVKFHENKVEYFLLGDCALFIKNGDSKVIKDRSICKFDNTVFDEMSKLSNLE
                     EMAFDEIRTSVMDTIVSNRLKKNTKEGYWILDFDEEAVENSIYGYIEIKKDFQLMLTS
                     DGFTSACDRYNLIKEEELIRIAEKFGIGYIHNKVRDFEDNDYKAVKVPRFKVKDDSSC
                     LYLDIYND"
     gene            11065..12300
                     /locus_tag="ACGI59_002280"
     CDS             11065..12300
                     /locus_tag="ACGI59_002280"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFG9740066.1"
                     /translation="MKILHIITQKPNSTGSGIYLSGMIKGFEKIGHKQAVIAGIDVND
                     DVNCFPSEVSFYPVKYNCGELNFPVVGMSDSMPYESTRYKDLNIDMINRLKYQFKVNI
                     DKAMNDFKPELIICHHLYLLTAFVREMVKDIKVMSICHGTCLRQLNTIDLEKEYIIAN
                     IRKLDLIFALHENQKYDIIKTFGVSESKVVVIGSGYNDDIFYNKNYKIKDDKIKIVFA
                     GKICKSKGLIPFIKAISKLKYSKDLIEVNFAGTGSDIESYNEIVKLASKSPFKMNFLG
                     KLEQRDLAELFNRSQIFVLPSFYEGLPVVVLEALSCGTDVITTDILGVKEWIGSEINN
                     SGKIEYVSLPFMEKEGIPKDEELYDFENNLYNAIDSKIQSLLNNSNKKTSVDMSKKTW
                     DGLAYRINEVILMDELCLV"
     gene            12401..13342
                     /locus_tag="ACGI59_002281"
     CDS             12401..13342
                     /locus_tag="ACGI59_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp25"
                     /protein_id="HFG9740067.1"
                     /translation="MRISKKVLALGISALLLSVSFPTSINALDKIENIQGVDKYETAG
                     LISDKQDFTTAVLINADSTMVDGIAASGLAGVNNAAILLTNKDDIPEATLQRLNRVTK
                     IYVIGGENSISKDVEKMLLMRRMQVIRIDGVDRVDTSYKIAGEIEKIKNSDKMFLVNG
                     FKDEADAVSVASVAYRDGAPIILTKDIPSAEEDTDLDPWFGVSPVPVYAIGGESTLSD
                     YIVSRYRATRIGGVDRYQTNKNVIEKFYNGAKEFYITSGDDLVYALVASPLAKNAPVV
                     LVSNKSDKSILSGASKVTAIGISDKSIIEQCLDAVKK"
     gene            complement(13561..13914)
                     /locus_tag="ACGI59_002282"
     CDS             complement(13561..13914)
                     /locus_tag="ACGI59_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFG9740068.1"
                     /translation="MDRKRELKQLYKEMKFDTGVFIIKNDITKKIYLGKSNDIKSKFN
                     SLKFQLGAGSCMIKELNNEWKKYGEKAFTFEVLELIKHDDNKTEKDYLEELDILEMVW
                     LEKLKEDNTYEIYSI"
     gene            14210..15121
                     /locus_tag="ACGI59_002283"
     CDS             14210..15121
                     /locus_tag="ACGI59_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HFG9740069.1"
                     /translation="MNKKIELIGNFASDWIRYSDYEYKITEEDELYIVPTEDAVFTMY
                     NPFDVADDIIVDIIRIGQEALQDISKETKEMKLKKEVLEFVKKYGLLGLIYSSVYNRD
                     IVGEERVLMIEKNYITKENILSTDEYVNMFIPFASDEDVYFKKYRRGIDVSKREESPK
                     FFGKRPLILDLVFSKFYTEQLDWIVSFAKDMCKHFNQICVYKNTSNLLTESVTIMSGG
                     FNPQKIGFSISQLDKPIIAWEFDSLKTAIETIYAFAITSEPSSLNKCKYCGKIYIPTN
                     SKSQYCSPSCRNCSNVKKSRSRAKKDK"
     gene            15381..15815
                     /locus_tag="ACGI59_002284"
     CDS             15381..15815
                     /locus_tag="ACGI59_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6323 family protein"
                     /protein_id="HFG9740070.1"
                     /translation="MISPIILSSINQNLKEIERNELLETNIESGDYGLALSESDVKDI
                     INSRDNTLKGYGRIELDIKVTKQLIENIYTSQYTNVDNYLEAINDMQEIFYYLKNETD
                     DKICDDEIIEILGEFYEKFSGNMDNVRGEADEFAKKFKFGEV"
     gene            15838..17373
                     /locus_tag="ACGI59_002285"
     CDS             15838..17373
                     /locus_tag="ACGI59_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6179 domain-containing protein"
                     /protein_id="HFG9740071.1"
                     /translation="MLNNLSNININENNLIKNQYSISLLKECLNCKVIDEREVYNIQQ
                     EVSLILMDLIKKYTNGQSTSVKTEVAEKLLISIWYAIDAYINKLGDIEKRVVELTAKN
                     VKKVYLGGIEILEEELVRLKDFYKLMMSRKLDTNSIAYNDTLMELSSFFKSYIVKFEA
                     QDVPVSIDYPLALDDMDVKGIYYVKNYIESIDIENRFCNLFKKKDREKLFYDYGMTYK
                     IDCDVILVNLFELIMNNCIFSTILGNEVTNLEISKYEYEFLEKQFKNMYNNITEDKVI
                     DSEESNIIELIEVDDSFNTEVDSNLDYNDFEEINREIRINKKITLLLMEAIEVLIKRL
                     SIEDEDVIRYIKKYEKIFIESVLSSIKSNTFKSMVTVSKNKKEHLEDYIVDDESKLDD
                     ESFREIFNEILDSTSIIEKIRIIKENINAKKDFIDILESECLFGEEYLMLFASLSELE
                     LAILGSVVFYEDIRMREINILEFMLNEKTETTLWKIEYIKFMKRQSEDKIYSIEEHMN
                     KIN"
     gene            17457..18824
                     /locus_tag="ACGI59_002286"
     CDS             17457..18824
                     /locus_tag="ACGI59_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFG9740072.1"
                     /translation="MKEYIINNVEEIREELIDLSKKIWENPELAFEEKYASSIQKEYL
                     KSKGFKIEEVENLPTGFIASFGEGKPVIGILGEYDALPELSQCVSAERKPLVEGKAGH
                     GCGHNLLGVAGVGAVVSIKKLIEENKFNGTIKYFGCPAEEEGGGKTVMCINGCFDDVD
                     CAFTWHPFDINAPWRGGSLANLSVKFKFKGITAHAAQAPHNGRSALDAVEIMNVGANY
                     LREHVIDSIRMHYVITNGGGRPNVVPGFAESWYFIRGKKAKDAEHVLDRLIKVAQGAA
                     MMTETEMEYKVTDGIYDYIPNQCLTDLVYDNMVFVGCPKNTPEEEEFAKKLCDTLTRE
                     ERLGVATSLQNDKSIVESYIHKEIVDGDKDKGLAGSTDVGDVSYVIPVAQFAMAAWPV
                     GIASHTWQSCSSAGSNIGFSAMINSAKVLACSAYDVFMDTKIIDEAKIEFDKSLDGQK
                     FKPLV"
     gene            18859..19083
                     /locus_tag="ACGI59_002287"
     CDS             18859..19083
                     /locus_tag="ACGI59_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015759013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HFG9740073.1"
                     /translation="MREKVEKVLEEKIKPVLQRDGGDVELIDVNENGVVLVRLQGACS
                     GCPGVTMTIKAIIENVLVSEVPGVTQVLGV"
     gene            complement(19533..19931)
                     /locus_tag="ACGI59_002288"
     CDS             complement(19533..19931)
                     /locus_tag="ACGI59_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HsmA family protein"
                     /protein_id="HFG9740074.1"
                     /translation="MNYKLILAIVFITSALIFYTIGVFGERKAKILKKKHVIIFWLGF
                     IFDTLGTFTMSNIANSHTFEVKSALSQNLHSITGLLAIVLMLFHASWATFVLYKDDEE
                     KKKFFHKFSIVVWTIWLVPYFIGMFIGMAG"
     gene            complement(19934..20377)
                     /locus_tag="ACGI59_002289"
     CDS             complement(19934..20377)
                     /locus_tag="ACGI59_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFG9740075.1"
                     /translation="MILLKSNYDCFRIAMLLKELYSKTMYTVEENFKENGLTHQQIIV
                     IKLIAHNQELTISQLCDEMSLAKGTVSGIISRLEQIGYIEKFKKSNDKRNTYVKFTTT
                     GFEFATNFKIKMQESFDDIFKNCDENELSDLVKNLRNILAKVKER"
BASE COUNT         8143 a         1987 c         3754 g         6560 t
ORIGIN      
        1 gaatgtaact taaattaaat tttagtagga attttatagg tttattttta aaggtttttt
       61 gaaaataaat ctatttaaaa attaaattaa aatgcaagaa aaacataata actttcattt
      121 ttaagaagtt ttcacaaaaa agaaaaaata tagtgcaata aatgatacaa agtaatataa
      181 tagattacaa gatatgaaaa gctaaaaaaa taaattgcaa atgaaattaa aatgaataaa
      241 aaataaaaaa ataatcataa gttagaagta tcaatgaaaa ataagactta atgtttttaa
      301 aattaaaagt atactaaggg gggcattaat atgtgcgtta tatcaaaaga tagagcaaag
      361 gaaataaaaa tagtagatac tactttaaga gatggagagc aaacagctgg ggttgtattt
      421 gcaaatagag aaaaaataat gattgctgaa atgttaagtg acctaggtgt tgaccaaatt
      481 gaagttggta tacctactat gggtggagat gaaaaaaatg taataaagca catatgtagt
      541 agaaatttaa aatcagatat aatggcttgg aatagagcag taataaaaga tgttgaagaa
      601 tctatttctt gtggtgttga tgcagtagct atatcaatat cagtatctga cattcatata
      661 gaaaataaat tgagaacttc tagaggctgg gttttagaaa atatggctaa aacagttgaa
      721 tttgcaaaga aaaatggatt atatgtttca gtaaatggag aagatgcttc aagagcagat
      781 atagacttct taactgaatt tataaatgtt ggaaaacaag caggggcaga tagatttaga
      841 tactgtgata cagtaggagt tatgaatcct ttttctataa agaatgcaat agaaactctt
      901 tatgaaagaa ctaattttga tatagaaatg catacacata atgactttgg gatggcaact
      961 gcaaatgcac ttgcaggaat agcagcagga gcaaattatg ttggtgttac agtaaatgga
     1021 ttaggtgaaa gagcaggaaa tgcagcatta gaagaagttt taatggcact taagtgtgtt
     1081 tataaatgtg atttaaataa tatagatact agaaaattta gaggaatatg tgagtatgta
     1141 gcacaggcat caggaagaat acttcctaca tggaaaccag tagttgggga taatatgttt
     1201 atacatgaat ctggtataca tgcagatgga gctttaaaag acccacataa ctatgaacca
     1261 tttgacccaa gtgaagtaaa tcttgaaaga aaaatagtaa taggtaaaca ttcaggaaga
     1321 gctgcagttg taaataagtt gagtgaatat gaaatgtata tttctccaga aaacgctacc
     1381 aagctattaa atgctataag agctacatct ataagattaa aaagaagttt aatggataaa
     1441 gaaattcttc aattatattg tgatatacta gcacatgaga aaggcacaac agaagaagaa
     1501 gcagttagag gttcatatat ttaagatata attgtttagg aggaaaatat gggagataat
     1561 atagtttata aaataattaa aaaacatata gtagatggag aagctgttgc tggaagttca
     1621 attggtataa aaatagacca aactttaaca caagattcta ctggaacaat gacatatctt
     1681 caactagaag ctatgggaat agataaagta aagactaaaa gaagtgtggc atttgtagac
     1741 cataatatgt tacaacaagg atttgaaaat gcagacgacc ataagtatat acaaacagtg
     1801 gcagataaat atggagttta tttctctaag ccaggaaatg gtatttgtca tcaagtattt
     1861 ttagaaagat tttcaacacc aggagataca cttttaggtt ctgatagcca cacacctact
     1921 gctggaggtg tgggcatgat ggctattgga gctggtggat tagatgttgc tttagccatg
     1981 gcaggaggag catactatat aaaagcacca aaagtctgta aggtgaatct agttggaaaa
     2041 ttaaataata tggtatcatc aaaagatata atattagaag tgttaagaaa acaaactgta
     2101 aaaggtggag taggtaaagt ttatgaatat ggtggagaag gtgtcaaaag cctatctgtt
     2161 ccacaaagag ctacaataac aaatatgggg gctgaacttg gagcaactac ttcaatcttc
     2221 ccaagtgatg aaaaaacact agaatttttt aagagtcaag gaagagaaga cgcatgggta
     2281 gagctaaaac cagatgcaga tgcagtttat gatgaagaga ttactataaa cttggatgaa
     2341 ttaaaaccat tagctgcaaa acctcatagc ccagacaatg tagatgaagt tgaaaatata
     2401 ggcaagatta aaatagacca agtggcaata ggaagttgta caaattcttc ttatgaagac
     2461 ttaatgaaag tagcacaaat cctgaaagga aataaagtac ataaggatgt gagtttggta
     2521 atagcaccag gttcaagaca agttatggag atgattgcta gaaatggggc tttggcagat
     2581 ataataagtg ctggagctag aatactagaa aattcttgtg gtccatgtat tggtatgggt
     2641 caatctccag gtactgactc tgtgtcttta agaactttca atagaaattt ctatggtaga
     2701 agtgggactt tgtcagcaca agtatattta gtaagtccag aagtggcagc agtaagtgca
     2761 atcaagggag tgcttacaga cccaagagaa tttgatatta aatttacaaa tttagatgta
     2821 aatgagtttt tgatagatga tagcatgata attaaacctg ctgatgtagg aagtgatgta
     2881 gaagtagtta gaggacctaa tataaaacct ttccctctta atacagagct aagtcagtct
     2941 ataggtggaa aagttatatt aaagacagaa gataatataa ctactgacca tataatgcca
     3001 tcaaatgcta agttacttcc ttttagaagt aatataccgt atcttgcaaa ctattgtttc
     3061 aatactgtag atacagaatt tcctcagaga gcaaaagaca ataatggtgg atttatagtt
     3121 ggtggagata attatggtca aggttcaagt agagaacatg cagctttagc accattatat
     3181 ctaggtgtta aaggggtaat agttaagagc tttgcaagaa tccacaaggc taacttgata
     3241 aatagtggta taattccaat ggagttttgt gatgagaaag attatgaaaa tatatctctt
     3301 ttagataatc tggagatacc aaatatttta gacaatctag gtagtggaat tttagaagtt
     3361 aagaatacaa ctaaaggaac ttcttttaag gtcaaagtag aattgtcagc aaaagaagtt
     3421 gatgtattaa aagctggtgg aaaattaaac tacactaaaa atcaagctaa ttaatatgaa
     3481 aagagggaat attgaatgta taaagtgaca cttataccag gagatggaat aggaccagaa
     3541 gttgcgaaag ctatgaaaaa ggtagtagaa gctactggag tagaaataga atgggaagaa
     3601 gttaatgcag gagaggcagt tattgaagag tatggtactc cacttcctga atatattata
     3661 gatagtataa aaaagaacaa aatagctata aaaggcccaa ttactactcc tgtaggtaaa
     3721 ggatttagga gtgtaaatgt aactttaaga caggcattag atttgtatgt caacttaaga
     3781 cctattaaaa gttttaaggg gataaaatca agatatgaag atgttgattt agtggttgta
     3841 agagaaaata cagaagattt atatgctgga atagaacata aaataggtga ttatgcagct
     3901 gaaagtataa agataataac aagaagtgca agtgaaagaa tcgttgattt tgcatgtaac
     3961 tatgttaaag ataataagag aaaaaaagtt acagctatac ataaggcaaa tataatgaaa
     4021 atgtctgatg gattgttttt agatgtgttt agagaagttg catcaaaaca tggtgtagaa
     4081 tatgatgatt tgattgtaga tgcagcagct atgaacttag ttttaaatcc agaaaattat
     4141 gatgtaatgg taatgccaaa tctttatgga gatatactat cagatttagg tgctgggctt
     4201 gttggaggtt taggtataat tccaagtgca aatattggta aagattgtgc gatatttgaa
     4261 gctgttcatg gaagtgctcc acaaatagca ggtcaaaata aggctaatcc aacagcactt
     4321 attcaatcat cagtaatgat gcttagatac ttaggtgaat atgaaaatgc acaaaaaata
     4381 gaaacagctt tagaaaaagt atttcttgaa ggaagtaaat tgactgtaga tttaggtggc
     4441 tcagcttcta ctacagagtt tgcggatgag gtgtgtaagt atattgtata gattttattt
     4501 agggaaaaag taaaatatag attgaagaaa agaggctttc tcaaaataga gataagcctt
     4561 ttttattgga atgataaaat atatttgatt ttatacaaaa gagagttcct cataaacgaa
     4621 aaagttaatt ttgggatatc cattttaatt ttaaaattaa ttgattaata cgcaaagaaa
     4681 gattaaataa atttattatt tttaattgaa ctttcaacca aaatatggta aaatttaaaa
     4741 agttgaactt tcaactaaaa atgaaaatat tgattatgtg aaattctata aaacagaaac
     4801 tatttgactt tagccatggt agtttcagtg gaggtgtgtt tgattaagac tttagatagt
     4861 aatatattaa gagaagtagg tactttatct agagcagtaa attcaataaa tgatattaag
     4921 tacaaggaat taaagttaca aaaaggacaa tttacatttt tgacaagaat ctgtgaaaat
     4981 cctggtataa atcttgtgga attatcaaat atgttaaagg tagacaaggc tacaacaacc
     5041 aaggctatac aaaaattaat aaaggcagga tatgtagata aaaagcagga taagtttgat
     5101 aaaagaggat ataatttaac tccaactgat aaatctttgg aagtttatga gttgataatt
     5161 gaagaagaga atagaagtat agagatatgt tttgataatt ttacagatga agaaaaacaa
     5221 gtggtaacta agttattaga aaaaatgagc aaaaacgtag aaaatgaatg gtttaaagta
     5281 aagagatagt aaataaatac attaacttaa ggagatattt ttatgattag aaaagcgaat
     5341 atgaatgatt tagaaagcat tatgaaaatt ataaaatcta cagtggaaga aatgaagact
     5401 tataataata cacaatggga tgaaaactat ccattagaaa aagactttgt ttcagatatt
     5461 aagaaacaag atttatatat ttatgaagtg gatggagaag tagcagggtt tatatgtctt
     5521 aattatgaag agccagaaga atatttaggc ttaaattggt catctaataa aaaagcaatg
     5581 gtaatacata gaatggctgt aaatccaaac tttagaaaga caggaatagc atctaagtta
     5641 gttgattttg ctgaaaaatt agctgtagaa aacaatgtat catacttaaa gagcgataca
     5701 tattcaataa actcaaaaat gaatttatta cttacaaagt gtggttttat aaaaatcgga
     5761 gaaatgagtt ttttaggaaa ggagaaatct ttctactgtt atgataaaat attataaaga
     5821 gcatttataa tacaaggggt aaataataga ttttcagcat tagtaagggc ataatataac
     5881 ctagtatatg ctattatgaa agggatgatt tatatgattg gatttctaaa gaaaagaaga
     5941 agtattagaa aatacaagga tgttgaagta gaaaaagaaa aattagataa aatattaaaa
     6001 gctgctcttt tagctccatc atcaaagggg cttagaacat gggaatttat agtggtagat
     6061 gataaagaaa agctaattaa tttatctcaa tgtagaacta aaggtggtgg atttttctta
     6121 aaaaatgcac ctttagctat tgtaattatt gcagataaag aaaaaaatga tgtttggata
     6181 gaggatgcaa gtatagctgc ttcatacata caacttcaag ctcatgaact aggtttaggt
     6241 tcttgttgga tacaagtaag aaatagaatg catgatgata atatagaagc tgataagtac
     6301 attagagaag aattaaaagt acctagcaag tacagtgtag aatgtataat ttctatagga
     6361 tatcctgatg aagaaaagaa agcatacggt gactctgatt tagactataa aaaggttcat
     6421 tttaacaact tttagtatat aatatgttgt attaagacat tatatttttt tctattgtta
     6481 atatatactt aaaattgtaa aaatatatct gaattttact aacttatcta ggggttatgt
     6541 acaaggattt taaagataaa tatagaaatt aatgtttata atcgatacat aacttgagta
     6601 acaaaattta aggaggttaa agtatgggta tatttaaaac aaaaatggat gaggactgga
     6661 aagtaaacta cattaaagag tttaatgaaa tgagagattc ttatgagagt aaacttcaaa
     6721 aaaaacagtt tgaggtagat agtttgaaat cagaattgga tagactaaga agctataaaa
     6781 atagccttaa gccaaaagaa aaacaaatta ctgatgatga tataaataat ataaagagtt
     6841 taagaagaga tggcttaagc tataaagaaa tttcaaatca aacaagttgg agcaaagcaa
     6901 ctgtaagtag ggtattaaat ggactttacg attagcatat acatggggtg atttaaattg
     6961 ggatgaagta tagttacaaa aaatgcataa tagattctat tttattgatg tttatagttc
     7021 aaatattaag gatgattttg aattatgtac ttctaagtca atttgaattt actcttgaaa
     7081 attttaatat tataaactta atttctttta cattagttgg tttatcgtta attttatttt
     7141 taaaagataa ttctttgtat aacaaggtta gaaatagaaa aatcacagaa gcttttgaag
     7201 aaaataaaaa caatatactt attgaaaagt gtaagctgat actttttgtt gtggttttat
     7261 ctttagcaat aattgtaact tattgtacta agggatatgt actttttaat gtaactatga
     7321 tgactttgtc tgtattgata gtcccaatat ttgaagagtt attttttaga gaatatatat
     7381 ggaactatct aagtaatttt ataaagtcaa agagtaaaat tatctgtata acatctatac
     7441 tatctggaat ttataatata ggatacatag atgttataag aaattatgta atattgtata
     7501 acaattcaag ctacaccttt gaagttatta tttcaaaaat tatgataggt actgtttttg
     7561 gtattgtact tggattagtt aagtataggt ttagagatgt agggttttgt atattattga
     7621 ggagcttatt tgctatattt attaggtaga tagtataatc ggtttataag gttgttttaa
     7681 atttaatttt aaatcaacct tattttattt gtgttttata aagcacttat atattaataa
     7741 ctaataaatt taataaaaat tatttgaata tttttaaaat atataaatat gtattgacat
     7801 ataaatatta tgaattacaa tttatataaa ctaataaaaa ttaaataata ttaatcaact
     7861 tattgaatgg aggaataaaa atgaaatttg tgactttttg tagtagcaat gaagaaaaaa
     7921 taggtgtatt taattcagaa acaaatagta tatatgaaat taatagttta ggattaagta
     7981 aattatatac tgatatgaat gattttattg aaaacgtatc tacaggagat ttagaaaaaa
     8041 taaaaaacaa ttcatttgaa aacgcaaagt gttataagtt agaagaagtt aaattatgtt
     8101 ccccaattgt aagacctaaa aaagatatta tatgtttagg tctaaactat aaagaccatg
     8161 tcaatgaaat cccagatgga gttataaaaa acgtagttat gccagattat ccaatttatt
     8221 tttctaagag agcagacaaa ataattggag tggatgataa gataagctta catggagatt
     8281 tggtagagaa gttggactat gaatcagaac ttgcagtaat cataggtaaa gaaggtataa
     8341 atatatcaaa agaagatgct tatgaatata tatttggata tacaatagta aatgatataa
     8401 gtgaaagagc acttcaagat aaacatgttc aatggtttag aggaaaaagc ttagacacac
     8461 atacatctat gggaccttgt atagtacata aagaagaatt tgaacatcct ttaaaattag
     8521 atataagtag tgtagtaaat ggagaagtaa gacaagattc taatacagag tactttatat
     8581 ttgatatacc tactataata aatgatttat caagaggaat gactctaaag ccaggagata
     8641 taatttcaac tggaactcca gcaggggttg ctatggggat gaatcctcaa gtatatttaa
     8701 agcatggcga tatagttgaa tgtaaagtcg aaggaatagg agttttaaaa aatatagttg
     8761 attaatggat aaatatgcca tataatttta ttagtacgaa taatttaatt aatataggat
     8821 taatcaaact tatgattaat ttattataga gagggatgag tatatggcaa gagaactaga
     8881 attaaagtat ggctgtaatc caaatcaaaa accatcaaag atttatatga agaatggaga
     8941 acttcctata gaggtattaa atggaaaacc tggatacata aattttttag atgcatttaa
     9001 tagttggcaa cttgtgaagg aattaaaaga agctacaggt cttccagcag caacttcatt
     9061 taaacatgta agtccagcag gagcagcagt aggggtacct ttatcagata ctttaaagca
     9121 aatatatttt gttgatgatt tagagctttc tccattagct tgtgcttatg caatggcaag
     9181 aggagcagat agaatgtctt cttatggcga ttttatagca ctttcagatg tatgtgataa
     9241 ggagactgca actattatag caagagaggt ttctgatggt ataatagctc caggatatac
     9301 agatgaagcc cttgaaatat taaaaggaaa gcgtaaaggt aactataata ttgtaaaaat
     9361 agatgaaaat tatactcctg aacctattga aacaaaggat gtttatggaa ttacttttga
     9421 acaaggtaga aatgaaatat taataaatga agacctatta aaagatattc caactgataa
     9481 taaaattttt acagatagtg caaaacgtga tttaattata gctcttatta cattaaaata
     9541 tacacaatca aattctgttt gttatgctaa agatggacaa gtaatagggg taggtgcagg
     9601 tcaacaatca cgtattcatt gtactagact tgcaggtaac aaagctgata cttggtatct
     9661 tcgtcaacat cctaaagtat taaacttaaa atttaaaaag gatataggtc gtccagatag
     9721 agataacact attgatgtat atctttctga tgattatatg gatgtgcttg ctgatggcat
     9781 atggcaaaac ttttttgaag aaaaaccaga gcctctaact ggagaaggaa aaagagcatg
     9841 gcttaaaact ttaacaggag tagcacttgg ttcagatgca tttttcccat ttggagataa
     9901 tatagaacgt gcaaaaagaa gtggggtaag ttttatagca caaccaggtg gttctattcg
     9961 tgatgataat gttatattaa cttgtaataa gtataacata gtcatggcat ttactaaaaa
    10021 tagattattc catcattaaa atactgaaag tataaagata atatagatta agtaaaaatc
    10081 tacaacaagt tttgttgtag atttttttat ttgctgtata attttaaata gaactttatt
    10141 tttagatatt caatttggag gttgatatgg ctttaaatat aagaaactat agcctattat
    10201 gtaatcaagg aacagctaaa tttaatgaag atgtagtcgg tataaatcca tttggggcat
    10261 gggttttaga tggtgctaca ggacttaatg gaaaaaattt agtgtcaact gaaagtgacg
    10321 caaggtggta tgttcagtgg tggaataggt atttatataa aaatataaat aaagaagaat
    10381 cattaaagag tattattaat actggaataa tagaagtaaa aaaagagtat gaactaagat
    10441 taaatgggtt taaggttgaa aaattagatt taccatctag ttctatagtt gttgtaaaat
    10501 ttcatgaaaa taaagtagag tattttttat taggagattg tgctttgttt attaaaaatg
    10561 gagattccaa agttataaaa gataggagta tttgtaaatt tgataatact gtatttgatg
    10621 aaatgagtaa actttctaac ttggaagaga tggcttttga tgagattaga actagtgtta
    10681 tggatacaat agtaagcaat agattgaaga aaaatacaaa agagggctat tggatattgg
    10741 attttgatga ggaagctgtg gaaaattcta tatatggata cattgaaata aaaaaagatt
    10801 ttcaattgat gctaacgagt gatggtttta caagtgcatg tgatagatat aatctaataa
    10861 aagaagaaga attgatacgt atagcagaaa aatttggtat aggatatata cataataaag
    10921 taagggattt tgaagataat gactataaag cggttaaagt gccaagattt aaagtaaaag
    10981 atgattcatc ttgtttatat ttagatatat ataatgatta gactgtataa aactatttaa
    11041 ttttagaaag gaggcaagtg aaaagtgaaa attcttcata taataacaca aaaaccaaat
    11101 agtacaggta gtggtatata tttgtctggt atgataaaag ggttcgaaaa aatcggtcat
    11161 aagcaagctg taattgcagg tatagatgta aatgatgatg taaattgttt tccaagtgaa
    11221 gtaagttttt atccagtaaa gtataattgt ggggagttaa attttcctgt tgtaggtatg
    11281 agtgattcta tgccatatga aagtacaaga tataaagatt taaatataga catgataaat
    11341 agattgaaat atcaatttaa agtaaatata gataaggcta tgaatgattt taaaccagaa
    11401 cttataatat gtcatcattt gtacctctta actgcatttg ttagggaaat ggtaaaagat
    11461 ataaaggtta tgtctatttg tcatggaact tgtttaagac agttaaatac cattgattta
    11521 gagaaagaat acataatagc aaatataagg aaattagatt taatatttgc tcttcatgaa
    11581 aatcagaaat atgatattat aaagactttt ggagtaagtg aaagtaaagt agttgtaatt
    11641 ggtagtggtt ataatgatga tatattttat aataaaaatt ataaaataaa ggatgataaa
    11701 ataaaaatag tatttgcagg taagatatgt aaatcaaagg gactgattcc atttataaaa
    11761 gctatatcta aattaaaata tagtaaagat ttaatagaag tcaattttgc aggtactgga
    11821 agcgatatcg aatcatataa tgagatagtt aaactggcaa gtaagtctcc atttaaaatg
    11881 aatttcctag gaaagttaga acagagagat ttagctgagc tattcaacag gtcacaaata
    11941 tttgtgttac catcatttta tgagggatta ccagttgtag ttttagaagc tttatcttgt
    12001 ggcacagatg taatcactac tgatattctt ggtgttaaag agtggattgg tagtgagatt
    12061 aataattctg gaaagataga atatgtttct ctacctttta tggagaaaga aggtattcca
    12121 aaagatgaag agttatatga ttttgaaaat aatttgtata atgcaataga ttctaagata
    12181 caatctttat tgaataactc aaataagaaa acatctgtag atatgtcaaa aaaaacgtgg
    12241 gatggcttag catatagaat aaatgaagtt attttaatgg atgaattgtg tttagtctaa
    12301 aattatttaa attataaaaa agtgtaacta tttttacact atttttaaat gattttatat
    12361 tatactaata ctaaatatat ttgttttagg gaggtaattt atgaggatta gtaaaaaggt
    12421 attggcgtta ggtatttcag cattactgtt aagtgttagt tttccaacat caataaacgc
    12481 actagataag attgaaaata ttcaaggagt agataaatat gaaacagctg ggcttatttc
    12541 agataagcaa gattttacaa cagcagttct tatcaatgca gactctacaa tggtagatgg
    12601 gattgctgca agtggtcttg caggagtaaa taatgcagct attttattaa ctaataagga
    12661 tgatatacca gaagcaacac ttcaaagact gaatagagtt actaagatat atgtaatagg
    12721 tggagaaaat tctataagta aagatgtaga aaaaatgtta cttatgagaa gaatgcaagt
    12781 tataagaatt gatggtgtag atagagtaga tacaagttat aaaattgcag gtgaaataga
    12841 aaaaattaaa aacagtgata agatgttttt agtaaatgga tttaaggatg aagctgatgc
    12901 agtaagtgtt gcatctgttg cttatagaga tggagctcct ataatattga caaaagatat
    12961 accttcagca gaagaagaca cagacttaga cccatggttt ggagtttctc ctgttccagt
    13021 atatgctatt ggaggagagt ctacattaag tgattacata gtgagcagat atagagcaac
    13081 tagaattggt ggtgttgata gatatcaaac taataaaaat gttattgaaa agttctataa
    13141 tggagcaaaa gagttctata taacaagtgg agatgattta gtttatgctt tagttgcatc
    13201 accacttgca aaaaatgctc ctgttgtttt agtttcaaat aaaagtgata agagtatatt
    13261 aagtggagct agtaaagtaa cagcaatagg tataagtgat aagagtatta tagagcaatg
    13321 tttggatgct gtaaagaagt aatggagtgt tattagtaaa atgaatatat acattgaaaa
    13381 ttagtagatt gttcatttta taatggtata aaatatatgg gaagaggcta tcaaatggag
    13441 ataagcctct ttttcattgt taaaattatg aaaagatact ttattttata taaaaaagta
    13501 tcttatgaac taaatgtcta ttttgagaca ctatctactt ttcttatatt atattatttt
    13561 ttatatggaa taaatttcat aagtattatc ttcctttaat ttttctaacc aaaccatctc
    13621 taaaatatct agttcttcta aatagtcttt ctctgttttg ttatcatcat gttttataag
    13681 ttctaaaact tcaaatgtaa atgctttttc accatatttt ttccattcat tatttaattc
    13741 ttttatcata catgagccag caccaagctg aaatttgaga gagttaaatt tgctttttat
    13801 gtcattactt tttccaaggt aaatcttttt agtaatatca ttttttatta tgaatacacc
    13861 tgtgtcaaat ttcatttctt tgtataattg ttttaattca cgttttctat ccatgatttt
    13921 agatacctct ctttataatt gatatgtaat atgtaagtgt aaaaatatat tacatatcaa
    13981 ttataaagag attaatttta tatgtaaata gtttttataa atttaattat gaattagtca
    14041 tcaataaaca agaaatttag ctgtaaaaat ccattataaa ggctattata aattttaata
    14101 tgctaaaaat ataaaaagaa tagttgacag tgtagaaaat atggaataaa ctaaaagtgt
    14161 aatagtaata tgaagttata tattacattg aaaggtgtga atgattatta tgaataaaaa
    14221 aatagaatta atagggaatt ttgcaagtga ttggattagg tatagtgatt atgaatataa
    14281 aattactgaa gaagatgaat tatatatagt tccaacagaa gatgctgtgt ttaccatgta
    14341 taacccattt gatgtagcag atgatataat agtagatata attcgcatag gacaagaggc
    14401 attacaagat atatcaaaag agacaaaaga aatgaaacta aagaaagaag tacttgaatt
    14461 tgtaaaaaag tatggattat tgggtttaat atattcaagt gtatataata gagatatagt
    14521 tggagaagaa agagtactga tgattgaaaa gaattatatt acaaaagaga atatattgag
    14581 tacagatgaa tatgtaaaca tgtttatacc atttgcaagt gatgaagatg tgtattttaa
    14641 aaagtataga aggggtatag atgtatcaaa gagagaagaa tctccaaagt tttttggaaa
    14701 gagaccattg attttagatt tagtattttc aaagttctac actgaacaac tagattggat
    14761 tgtaagtttt gctaaagata tgtgtaagca ttttaatcaa atttgtgtat ataaaaatac
    14821 atctaatcta ttaactgaaa gtgttactat tatgtctgga gggtttaatc ctcaaaagat
    14881 aggattttct ataagccaat tagataaacc aattattgca tgggagtttg attctttgaa
    14941 aacagctatt gagactatct atgcctttgc aattacaagt gaaccttctt ctttaaataa
    15001 atgcaagtac tgtggtaaaa tttatattcc tacaaattct aaatctcaat actgttcacc
    15061 ttcttgtaga aattgctcaa atgtaaagaa aagtagaagt agagccaaaa aagataaata
    15121 aaattaataa atatatcatt gtttagattt tagtaaataa ctactgaatt aaaagttgtt
    15181 atagaaaatt atatatctaa atgatggtat ttttttgttg agaaaattga aaagtttaat
    15241 tgagatagta attttgagtt gacaaaaacc acttttaaca ctactattat aagtggttgg
    15301 taaaataata tacattaatt taagtatagc ataaaaaaca aaaaaagtca atggaaaaaa
    15361 taaaaaagga gaatttaaaa atgatatcac ctattatttt gagttctata aatcagaatt
    15421 taaaggaaat agaaagaaat gaactgcttg aaactaatat agaatctgga gattatggtc
    15481 tagctctttc tgaatctgat gtaaaagata taataaattc gagagataat actttaaagg
    15541 ggtatggaag aatagaatta gatataaaag ttacaaaaca gttaatagaa aatatatata
    15601 catcacaata tacaaatgtg gacaattatt tagaggctat taatgatatg caagaaattt
    15661 tttattattt aaaaaatgaa actgatgaca agatatgtga tgatgagatt atagaaatac
    15721 tgggtgaatt ttatgaaaaa ttttcaggca atatggacaa tgtaagagga gaagcagacg
    15781 aatttgcaaa gaaatttaaa tttggagaag tttaatatat gataggagag attttgtatg
    15841 ttaaataatt taagtaatat taatataaac gaaaataatt taataaagaa ccaatattcc
    15901 atatctctac ttaaggaatg tctaaattgt aaagtgatag atgaaagaga agtatataat
    15961 atacaacaag aagtatctct aatacttatg gacttgatta aaaaatatac aaatggtcaa
    16021 agcacatcag taaaaacaga agttgccgaa aaactgttga tttcaatatg gtatgctatt
    16081 gatgcatata taaataagct tggagatata gaaaaaagag ttgtggagtt gactgctaag
    16141 aatgttaaaa aagtgtacct tggaggcatt gaaatattag aggaagagtt agttaggcta
    16201 aaagattttt ataaactaat gatgagtaga aaattggata ctaattcaat agcatataat
    16261 gataccttga tggagttatc aagtttcttt aaaagttata ttgtaaaatt tgaagctcag
    16321 gacgttccag taagtataga ttatccattg gcactagatg atatggatgt taagggtatt
    16381 tattatgtga agaattatat tgagagtata gatatagaaa atagattttg taacctattt
    16441 aagaaaaaag atagagaaaa attattttat gactatggga tgacttataa aatagattgt
    16501 gatgttatac ttgtaaattt atttgaactt attatgaata attgtatttt ttctactatt
    16561 ttaggaaatg aagtgactaa tttagaaatt tcaaaatatg agtatgaatt tttagaaaaa
    16621 caatttaaaa atatgtacaa taatattact gaagataaag ttatagactc tgaagaaagt
    16681 aatataattg aattgattga agtagatgat agttttaaca ctgaagtaga tagtaatttg
    16741 gattataatg attttgaaga aattaacaga gaaattagaa taaataaaaa aataacttta
    16801 ctacttatgg aagcaattga agtattaata aaaagattaa gcatagaaga tgaagatgta
    16861 ataagatata ttaaaaaata tgaaaagata tttattgaat ctgtgctaag ctcaataaaa
    16921 agtaacactt ttaaaagtat ggtaactgtg agtaaaaata aaaaggaaca cttagaagat
    16981 tatattgttg atgatgaaag taaattggat gatgaatcgt ttagagagat attcaatgag
    17041 atattagata gtacatctat aattgaaaaa ataagaataa taaaggaaaa tatcaatgct
    17101 aaaaaagact ttatcgatat tcttgaatct gaatgtttat ttggagaaga gtatttaatg
    17161 ttgtttgcat cattaagtga attagaattg gcaatattgg gtagtgtagt attttatgaa
    17221 gatattagaa tgagagaaat taatatatta gaatttatgt taaatgagaa aactgaaact
    17281 actttatgga agatagagta cattaaattt atgaagaggc aaagtgaaga taagatatac
    17341 tccatagaag agcatatgaa taaaattaat taacaaaaaa ttgtgaataa atagaatgtt
    17401 agtatataat ataatctgta aagcatatca aaaatacata tatttggagg aaaattatga
    17461 aagagtatat cataaataat gttgaagaaa taagagaaga attaattgat ttatctaaga
    17521 aaatatggga aaatccagaa ttagcctttg aagaaaagta tgcaagtagc attcaaaaag
    17581 aatacctaaa gtcaaaagga tttaaaattg aagaagtaga aaatttacct actggattta
    17641 tagcaagttt tggagagggt aaacctgtga ttggcatact tggagaatat gatgctttac
    17701 cagagctatc acaatgtgta agtgcagaaa gaaagccatt ggtagaagga aaagctggac
    17761 atggatgtgg gcacaacctg ttgggtgtag caggtgtagg agcagttgtt tctataaaaa
    17821 aattaataga agaaaataaa tttaatggaa caataaaata ttttggttgt cctgctgaag
    17881 aagaaggtgg aggaaagaca gttatgtgta taaatggctg ttttgatgat gtagattgtg
    17941 catttacatg gcatcctttt gatataaatg caccttggag aggtggaagt ctggcaaact
    18001 tatctgtaaa atttaaattt aaagggataa cagcccatgc agctcaagct cctcataatg
    18061 gtagaagcgc acttgatgct gttgaaatta tgaatgttgg agctaattat ttgagagaac
    18121 atgttattga ctctataaga atgcactatg ttataactaa tggtggtgga agacctaatg
    18181 tagttcctgg atttgctgaa agttggtact ttattagagg taaaaaagca aaggatgcag
    18241 aacacgttct tgatagatta ataaaagtag ctcaaggagc agcaatgatg acagaaactg
    18301 aaatggagta taaggttaca gatggaatat atgactatat accaaaccaa tgtttgactg
    18361 atttagttta tgacaatatg gtttttgttg gttgccctaa aaatactcct gaagaagaag
    18421 aatttgcaaa aaagttgtgt gatactctta ctagagaaga aagattaggt gtagcaacat
    18481 cattacaaaa tgataaatct attgtagaaa gctatataca caaggaaata gttgacggtg
    18541 acaaagataa aggactagct ggttctactg atgttggaga tgtaagttat gtaataccag
    18601 tggctcaatt tgcaatggca gcttggcctg ttggaattgc aagtcataca tggcagtcat
    18661 gttcatcagc aggttcaaat ataggatttt ctgctatgat aaattcagct aaagtactgg
    18721 cttgtagtgc ttatgatgta tttatggata ctaaaattat tgatgaagca aaaattgaat
    18781 ttgataaatc attagatgga caaaaattta aaccattggt ataattttgc taataaaata
    18841 tttctggggg gaatagctat gagagaaaaa gttgaaaaag tgcttgagga aaagataaaa
    18901 ccagtattac aaagagatgg tggagatgta gagcttatag atgtaaatga aaatggtgtg
    18961 gttttagtta gactacaagg ggcatgtagt gggtgtccag gagtgactat gacaatcaaa
    19021 gctattattg aaaatgtttt ggtgagtgaa gttccaggag taactcaagt attaggtgtt
    19081 taatagttag cgtttattaa atgtatgtta tacataagta tttaataaag aagtaaaaaa
    19141 aatagtgaag caatatatat gaaaaagtat gagaaagata ataaaacatt atagaaataa
    19201 tgaaatattt catatgtata tgaagaaata agaaaaaata gtgaagcaat atatatatga
    19261 aaaagtatga gaaagataat aaaacattta tagaaataat ggaatatttc atatatacat
    19321 atgaagaaat agtaaaacaa tatatatatg aaaaagtgtg agaaaataat aaaatactat
    19381 agaaacaatt aaatattcta tataggtatt aagaaacatg aaagaaataa tggagtatta
    19441 taaataaata tgtaaaagtg tgaaagaagg atgtagataa atttgtctta tacaaattta
    19501 cctacagtat ttcttacaca ctttttattt tactatccag ccataccaat aaacatacct
    19561 ataaaatatg gaactaacca tatagtccat acaactatac taaacttatg aaagaatttt
    19621 ttcttttcct catcatcttt gtataggaca aaagtagccc aacttgcatg aaaaagcata
    19681 agcacaattg caagaagacc tgtaatacta tgaaggtttt gtgataaagc agattttact
    19741 tcaaaagtat ggctgttggc tatgttactc attgtaaatg ttcctaaagt atcaaatata
    19801 aaaccaagcc aaaatataat tacatgtttc ttttttaaaa tttttgcctt tctttctcca
    19861 aatacaccta tagtgtagaa aattaaagct gatgttataa atactattgc aagtattaat
    19921 ttataattca taattatctc tcctttactt ttgccaatat atttctaaga ttttttacta
    19981 agtcactcaa ttcgttttca tcacaatttt taaaaatatc atcaaaactt tcttgcattt
    20041 taattttaaa atttgtggca aattcaaatc ctgttgttgt aaacttaacg tatgtatttc
    20101 ttttatcatt agatttcttg aatttttcta tatatccaat ctgttctaat ctgctgatta
    20161 tgcctgaaac cgtaccttta gctaaagaca tttcatcaca tagttgtgat attgtaagtt
    20221 cttgattatg agctatcagt tttataacta taatctgttg gtgtgttaat ccattttctt
    20281 taaaattttc ttctactgtg tacatagttt tagaataaag ttcttttaag agcatggcta
    20341 ttctaaaaca gtcataatta cttttcaata aaatcacctc ttaaatggtt tgcatacaaa
    20401 ctaatataat atacttttct tatatagtca acctaaaatt ttat
//
LOCUS       DAYAPA010000068        20202 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.068, whole genome shotgun sequence.
ACCESSION   DAYAPA010000068 DAYAPA010000000
VERSION     DAYAPA010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20202
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.068"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            78..776
                     /locus_tag="ACGI59_002290"
     CDS             78..776
                     /locus_tag="ACGI59_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9740076.1"
                     /translation="MKISSQYRSQYSFRYESNINNTRINESMVKKNETVGKDTYLSNI
                     MKQKQELNDRIRDLKYRQEVYTKKINDAIKNLCKSEIRETTNNFSNIEIGIKNSIIEE
                     KNKSTMLDENSTYLNTNDEKESLITKESNEKIEEEILNDEKLEELEQKKDYKEDSNKK
                     EKVSEDLSLVGKTREELENMLKNFINLTQEEIMKLESRIEKLDKNAEEYKQNSKTNIF
                     DKTDEQKKHINVLI"
     gene            954..1844
                     /locus_tag="ACGI59_002291"
     CDS             954..1844
                     /locus_tag="ACGI59_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9740077.1"
                     /translation="MKKNILTTMITMIIILMMSMTVMAEPYQEDETSSLKKQSANERN
                     QTNIGTMDGRVQTNAISKGANYDPKYPLKGMLTQLGLNTTGKEGWDLLYGGNPNGVEL
                     RADGKYYYINKGMEGLGFYAAALSGGSYQRIGFGKLDPIEVIADGNWISYKQAQDTLT
                     VIRNFLNSFDWRNASEMERANRAAKLVTEAKYADSKYCNIVYGNLVDKRGVCGSFASS
                     FHLLTRLMGMDSLSILNPSLNHAWNYIQIDGKWYRFDGSEISAFGGSLDFDYRKLKDA
                     TREMTTYYDAKALSILGFNQ"
     gene            complement(2237..3193)
                     /locus_tag="ACGI59_002292"
     CDS             complement(2237..3193)
                     /locus_tag="ACGI59_002292"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFG9740078.1"
                     /translation="MENNTIQKQLRDLSKKIWHIYILNPEEENLKFIISLLDDNLALI
                     GTGKHEFYETLEEFIIGLEKDQTESEQIDFEIIDEWYSCTKVTDEAFIVYGTLWVIEK
                     DYLEKDIYVEMDTRFTMVYRVTDDMIKLIHVHHSIPNFDQMKDEYYPKTITEKAQEAL
                     AMANEFKLKSEKDLMTGLYNRHFFEVHIKEMLKTVSHGYFYIFDLDNFKQVNDIFGHV
                     RGDEILIHFSNILTKVFVNDAIIGRLGGDEFAVFEYSNQSKENVENKAKKVLELCLES
                     NSCSIGISKLENNSDTFQSLYKQSDYALYYSKRNNKGNFSWF"
     gene            3896..4891
                     /locus_tag="ACGI59_002293"
     CDS             3896..4891
                     /locus_tag="ACGI59_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFG9740079.1"
                     /translation="MSIIELNELTKAYRRFEKKAGFMGSVKSLFNRKYTEKTAILSFD
                     LKIEEGEFVGLIGQNGAGKTTLIKMLTGIIAPSSGEISVMGYYPNKLENNFKKQYAVV
                     MGQKSQLFFELTPADTFLLFKELYDISNEEYKKNLDYFIELFEVSDYLNVQVRTLSLG
                     ERMKMELIVALLHNPKILFLDEPTIGLDAIAQKQIRYFLKEVNETKGTTIILTSHYME
                     DIKSLCSRSIVVENSRKIYDGSTEKLFNHFQKYKKITVSFSNETNIVFPKDCKVVEQT
                     NHKISVIVPKERGNETIKWIFDKYDVKDVMIEEEDIGSIVERIYKKGSDEYYEQL"
     gene            4878..5732
                     /locus_tag="ACGI59_002294"
     CDS             4878..5732
                     /locus_tag="ACGI59_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9740080.1"
                     /translation="MSNYNEASSNDSMFYKFKQSIRRYYEVAKINFKVQLIWRFDVVV
                     NMVFTIVKILFAYILWKAIFGENKIVSGFTFNSMLSYYIISSFLAGIDMSKSTSEEIC
                     NRVKLGTFSKYMIIPINVQNYFIAKNFGMTCFYFVFNMISAFVWIFIFKIDFAFTSNP
                     VQILISILMIFLGFIFMMKLHFFIGILSFKFLEISFFKMIIENIVEFITGTMIPLVLL
                     PETIVTVMKVFPFYYTTYLPSMLLIGRNSNEAIQGLIILALWVLFFSVVNKVMYKRMR
                     TLFDGVGI"
     gene            5732..6523
                     /locus_tag="ACGI59_002295"
     CDS             5732..6523
                     /locus_tag="ACGI59_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HFG9740081.1"
                     /translation="MKKNLKIIFMLLKMKLSKMMVFRFDFFGAFFVDSSLFILQLLMF
                     NSIYSHVDSIGGWQQGEMLIFIGTFSLINAINMTIFFFGIYDIPRKIQEGELDYYITK
                     PLDPLFRLTFENINIGSSPLILASILIVLYGISELNSEITLVMIIIYIFMVLLMTLLF
                     YDVCIILRTVSFFVISSSAIMRLEDNLLPMNMKIPGVIYEGVFKVLFYLILPYGIMST
                     IPTQILAGTITINGLIYSILIVFLFTLFMWRFWKLGMRHYKSASS"
     gene            6808..8187
                     /locus_tag="ACGI59_002296"
     CDS             6808..8187
                     /locus_tag="ACGI59_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFG9740082.1"
                     /translation="MSDVYYSENHEQDNLYEQDNLLAQRFNLFTLLKFAFPTIFMMIF
                     MGLYTIVDTIFVSRFINTNALSAINIVCPIINLIVGIGTMLATGGSAIVARKMGNGDI
                     EGANKAFSLIVLSGVVIGVIITGIGIVFMDYIIIWLGASEVLIKYCRSYLFIILVFAS
                     ANILQVLFQCLFVTAGKPQFGLWLVLGAGCANIILDYVFIVLLQMGISGAALATGVGY
                     LIPTVSGMVFFSKNKGLLKFCIPRLDKRILFEACFNGSSEMVGQLSTAVTTFLFNAMM
                     MKLIGEDGVAAITIIIYSQFLLTSLYIGFSMGVAPIISYNYGSENNTQLNHVFKICIL
                     FISVASLLVFIFSISCSPIIVEIFSQRGSRVYEIARSGFYIFSFSFIFCGYNIFTSSM
                     FTALSNGKVSAILSFLRTFGFITIGLLILPNILNINGIWLAVPSAEILTLFLTIFCIC
                     KYKNRYKYM"
     gene            8635..9348
                     /locus_tag="ACGI59_002297"
     CDS             8635..9348
                     /locus_tag="ACGI59_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFG9740083.1"
                     /translation="MDTCFNKKILLVDDEKDIVDLIEEVLINDGFKNIIKAYNGLDAI
                     SLCKIACPDVVILDIMLPDIDGIEVCKKIREFSYCSILFLSSKNDDIDKILGLSSGGD
                     DYITKPFSPREIAFRVKAQLRRQQYQSIVPSDSEVIKIGDITIDIEGNRVYKDRNEIE
                     LTGREYHLLSYMAQNVNKIIGKERLYEQVWGVYSSICDNTIMVHIRHIREKIEDNPSN
                     PKILITVKGLGYKLVNRID"
     gene            complement(9620..11158)
                     /locus_tag="ACGI59_002298"
     CDS             complement(9620..11158)
                     /locus_tag="ACGI59_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFG9740084.1"
                     /translation="MSLLKVTNLSQCFMDKSLYEKANFDLFKGEHIGVVGQNGTGKST
                     LIKILLGEVVPDSGEIKWQPNINIGHLDQYAEINRDTTISLYLHTAFEELYKIEKEMN
                     LLYQKSAISENEQYLIKASDYQEQLIASNFYSIDNEINKIANGLGLDSIGMNRVVGEL
                     SGGQRAKVILAKLLLSNHDVLLLDEPTNFLDKEHVEWLSNYLNTFNGAFIVVSHDFDF
                     LEKISTGILDIEFGMIKKYHGKYSEFLKQKSHLREDYIRRYQAQQKKIEKEETFIRKN
                     KAGVNSKIARGRQKQLDKIERIAPPSFTGKPNIQFSEIEISAQNALTITNLEVGYYYS
                     LLPKLNFSVDGGQKIVITGFNGIGKSTLLKTLVKDIPRISGDFQFSEQVKIGYYEQDL
                     KWENPDKTPLQIVADKYPKLNTKEIRRHLARCGVKEEHVSRSVSTLSGGEQSKVKLCC
                     MMLSPCNFLILDEPTNHFDAETKDALQNALKQFRGSIILVSHEEKFYKGWIDKVFNIE
                     KQLV"
     gene            12049..12174
                     /locus_tag="ACGI59_002299"
                     /pseudo
     CDS             12049..12174
                     /locus_tag="ACGI59_002299"
                     /inference="COORDINATES: protein motif:HMM:NF024804.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AsnC family transcriptional regulator"
     gene            12229..12495
                     /locus_tag="ACGI59_002300"
     CDS             12229..12495
                     /locus_tag="ACGI59_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HFG9740085.1"
                     /translation="MAFIFINTDLSKSMKEFKKTILEYDTVLECHHISGEYDYLIKVL
                     AEDTSNLEEFISVTLKEIGGIQKANTTIVLSTIKEEFNTIKDIF"
     gene            12544..13128
                     /locus_tag="ACGI59_002301"
     CDS             12544..13128
                     /locus_tag="ACGI59_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HFG9740086.1"
                     /translation="MKVLVFLAKGFETMEFSVFVDVMGWARNDYGHDIDVVTCGFKKQ
                     VMSTFNIQVLVDKTIEEVCVDDYDALAIPGGFEEFGFYDEAYDSSFLNLIREFNSKEK
                     IIASICVAALPVGKSGVLKNRKATTYHLKNGKRQRQLSEFDVNVVNEPIVVDKNIITS
                     YCPETAPHVAFKLLEMLTSKEQMDEVKLAMGFKL"
     gene            complement(13133..13456)
                     /locus_tag="ACGI59_002302"
     CDS             complement(13133..13456)
                     /locus_tag="ACGI59_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFG9740087.1"
                     /translation="MKKCLDNYSCPIEATLALIGGKYKTLILWHLKDTILRFNELKKL
                     IPKATPKMLTQQLRELESDGLIIRVVYPVVPPKVEYSLSDFGKSIIPILDSMCDWGSD
                     YLENL"
     gene            13608..14126
                     /locus_tag="ACGI59_002303"
     CDS             13608..14126
                     /locus_tag="ACGI59_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFG9740088.1"
                     /translation="MNFVELAKKRYSCRNYQDRKVEKEKLEKVLDVARIAPTGGNRQP
                     QRLIVIQEKEGINKLSKAANIYDAPLAILVCGDKDKVWTRPFDGKQLTDIDTSIVTDH
                     MMLQATELGLASVWVCYFNPDIIREEFSLPDNLEPINILLMGYESKIPESPERHEKTR
                     VPLSEIVSYETL"
     gene            complement(14302..14970)
                     /locus_tag="ACGI59_002304"
     CDS             complement(14302..14970)
                     /locus_tag="ACGI59_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HFG9740089.1"
                     /translation="MLEISHKVKEIYPNIKFGVMVINITHSKPNKENFLVLKNSTIKN
                     IIEQHPEYNRKEKIKTEPVSSYIKYYKKFKKTYPVLLQLESILLKSKGIPDVGVTIES
                     MFLAELKNLLLTAGHDLDKMELPLKIELANGSEHFYGIGGKEHILTKDDLFLSDNIGI
                     LSSILNGPDNRTAITKDTKNIMYFVYGPDKISEKQIQDHLNDIKHYISSAFPDLKVNS
                     IDIF"
     gene            15085..16500
                     /locus_tag="ACGI59_002305"
     CDS             15085..16500
                     /locus_tag="ACGI59_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFG9740090.1"
                     /translation="MWITINRNSNISLSRQIYCEIKDMILDGSLKFEQKLPSSRTLSK
                     ELSVSRNTVLDAYNQLIAEGYLETRNGYGTIVAKGISGHKVDFKLNDTFRDEKDLHTN
                     KQKNYIDFRSGVPELKLFPRKELSKLYKQILYDLSDSDFRYGSTAGVWVLRVEISKYL
                     FRTRGIRCNPKNIMIVSGSTQGLSLISQLLYKSNKKVAVEDPIHKGLLKVISSIGYSV
                     VGIKVDDKGMNTNLIKSIKGISFVYTTPSHQYPLGGILPIQRRLELIKCATENDYYII
                     EDDYDSEFRYEGQPISSLYELSPNKVIYIGSFSKILSPAIRVGFMLLPDNILESYKTL
                     KMYSDVHTETILQHVLAEFIGNGGLEKYIFRMKKLYNRKRKYLIKVLNTEFKNEFKIK
                     GEAAGLHIVAHFYNVKFTESLIHKIFEAGIKVYPIQQYAIENILEYDYEIILGYAHLS
                     FDEIEKGVKILSKIIHMNQGT"
     gene            16699..17181
                     /locus_tag="ACGI59_002306"
     CDS             16699..17181
                     /locus_tag="ACGI59_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFG9740091.1"
                     /translation="MKTNTDVIMEIIDYIEENLTENLNLDKIAYIANYSKYHLHRMFT
                     NVVGCTIHQYIQRRRLTEAARQLIFTDKYIIDIAITVGYSTHQSFTLAFRNLYKESPQ
                     VYRNKDEFYPIQQRFVLNLNHQIQSEKCISYANMNYKQRRDEINSYLNFNINCEVKVA
                     "
     gene            17178..17762
                     /gene="clpP"
                     /locus_tag="ACGI59_002307"
     CDS             17178..17762
                     /gene="clpP"
                     /locus_tag="ACGI59_002307"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFG9740092.1"
                     /translation="MSLVPHVIEQTGQGERSYDIYSKLLKDRIIFIGEEINDAIASLV
                     VAQLLFLESEDPDSDIIIYINSPGGSATSGFAIYDTMNYVRCDVSTICIGMAASMSAF
                     LLAGGTHGKRCALPNSEIMIHQPMGGAKGQATDVKIAVDNILKIKERLDKILSENTGK
                     SIEDIRRDTDRDNFMTALEAKEYGLIDYIMNRNE"
     gene            18074..19150
                     /locus_tag="ACGI59_002308"
     CDS             18074..19150
                     /locus_tag="ACGI59_002308"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFG9740093.1"
                     /translation="MITISLCMIVKNEEEVIGRCLECVKDIVDEIIIVDTGSTDSTKE
                     IVSKYTDMVYDFEWIDDFSAARNFSFSKASKDYTMWLDADDIILEADREKLLKIKESL
                     DTSIDIVMAKYNVSFDENGNPTLSYYRERLFKRSSNHKWVDPIHEVVPLFGEVFYSDI
                     AISHKKLHRQDPLRNLRIFEKMISEGKTLEPRHQFYYSRELYYNARYKEAIEGFTKFL
                     DSSRGWIEDCISACRDLATCYYLINDEKSALYSLFRSFEFDEPRAEICCDIGKHMFDR
                     QKYKEAIFWYKVALTRDKNDTDGGFKSDDCYGYIPSIQLSVCYDRLGESDKAIYYHEK
                     TKEIKPNDSAVLHNENYFSKFKNS"
     gene            19217..>20202
                     /gene="bclA3"
                     /locus_tag="ACGI59_002309"
     CDS             19217..>20202
                     /gene="bclA3"
                     /locus_tag="ACGI59_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like exosporium glycoprotein BclA3"
                     /protein_id="HFG9740094.1"
                     /translation="MLLIMSRNKYFGPFDDNDYNNGYDKYDDCNNGRDDYNSCDCHHC
                     CPPSCVGPTGPMGPRGRTGPTGPTGPTGPGVGATGPTGPTGPTGPTGNTGNTGATGLR
                     GPTGATGATGPTGATGAIGFGVTGPTGPTGATGATGADGVTGPTGPTGATGADGITGP
                     TGATGATGFGVTGPTGPTGATGVGVTGATGLIGPTGATGTPGATGPTGAVGATGIGIT
                     GPTGPTGATGADGATGVTGPTGATGATGADGVTGPTGATGATGFGVTGPTGPTGATGI
                     GITGATGLIGPTGATGATGATGPTGVTGATGAAGLIGPTGATGATGADGATGA"
BASE COUNT         7499 a         2384 c         3299 g         7020 t
ORIGIN      
        1 tatacttttg atgataaaat ataattaagt taagttggtt tttataaaca taaatttatt
       61 ttaaggaggt gaaataaatg aaaatatcta gtcaatatag aagtcaatat tcatttagat
      121 atgaaagtaa tataaataat acaagaataa atgaaagtat ggttaagaaa aatgaaactg
      181 taggaaaaga cacttattta tctaatatta tgaaacaaaa gcaagaactt aatgatagaa
      241 ttagagattt aaaatataga caagaggttt atactaaaaa aataaatgac gcaattaaga
      301 acttatgtaa atcagaaata agagaaacaa ctaataattt ttctaatata gaaataggta
      361 ttaaaaatag cattatagaa gagaaaaata aaagtacaat gttagatgaa aattcaactt
      421 atctaaatac aaatgatgaa aaagaatctt taatcactaa agagtctaat gaaaaaattg
      481 aagaagaaat attaaatgat gaaaaattag aagagttaga acaaaaaaag gattataaag
      541 aggattctaa taaaaaagag aaagtatctg aggacttatc tttagtaggt aaaactcgtg
      601 aagagcttga aaatatgctt aaaaatttta taaatttaac acaagaagaa ataatgaaac
      661 ttgagtcgag aatagaaaag ttagataaaa atgctgaaga atacaaacaa aattcaaaga
      721 ctaatatatt tgataaaaca gatgaacaaa aaaaacatat aaatgtactg atttaaacaa
      781 tatgttttaa aaaaataagt ttattatata atgaaaatat aaaggtacaa tttcaataca
      841 cttctattaa accaataatt tatgaaccag acttaatttc aatttactaa taaccatata
      901 tttatgagtt aatagtttta tgttttaaaa agaattaaat aagggggagt agtatgaaga
      961 agaatatatt aactactatg attaccatga ttattatatt gatgatgagt atgacagtaa
     1021 tggcagagcc atatcaagaa gatgaaacat ctagcttgaa aaaacagagt gcaaatgaac
     1081 gtaatcagac taatataggc acaatggatg gcagagtgca aacaaatgca ataagtaaag
     1141 gtgctaatta tgaccctaaa tatcctctta aaggaatgtt aacacaactt ggtcttaata
     1201 caacaggtaa agagggatgg gatttactct atggaggaaa tcccaatgga gttgagcttc
     1261 gagcagatgg taaatattat tatataaata agggtatgga agggctagga ttttatgcgg
     1321 cagcattgtc aggtggctca tatcagagaa taggctttgg aaaacttgac cctatagaag
     1381 taatagctga tggaaattgg atttcttaca aacaagctca agatacactt acagtaatca
     1441 ggaatttttt aaatagcttt gactggagaa atgcatctga gatggaaaga gctaatagag
     1501 ctgcaaaatt ggtaaccgaa gcaaaatatg cagactctaa atattgtaac atagtctatg
     1561 gtaatctggt tgataaacgt ggtgtatgtg gaagttttgc aagtagcttt catttattaa
     1621 caaggttaat gggaatggat tcactatcta tattaaatcc atcattaaat catgcttgga
     1681 actatataca aattgatggg aaatggtata ggtttgatgg gtctgaaata tcagcttttg
     1741 gtggatcatt agactttgat taccgtaaat taaaggatgc aacaagagag atgactactt
     1801 actatgatgc taaggcattg tctatattag gatttaatca ataaaaatag ggggaggctt
     1861 cttatagagc ctttttttat tgatttgaaa tttaataagg aattttatat gaaaatttaa
     1921 tcaataaaag caaaaaaggc tctttataaa gcctttctaa gtgttttttt aagtaaaatt
     1981 ttttatatca acatcagttt taatcaaaac atataacatt ttggacttac ttttaaaaat
     2041 aaaattatta aaatataata ttaatcatgt ttttagttta ttatattcta taatagtggt
     2101 catgttgact attgtagttt tattaagaat agttggttta tataaatata tgcaaagata
     2161 gcttgttgtt tactttactt attagtatat aggacaagat actctaataa atttgcctat
     2221 tttaatatct tttgtactaa aaccaactaa agtttccttt attatttcgt tttgaataat
     2281 acaaagcata atccgattgt ttgtataaag actggaaagt atcagagtta ttttctaatt
     2341 tagatatacc tatagaacaa gaattactct ctaggcataa ttctaaaacc ttctttgcct
     2401 tattttcaac attctctttt gattgattac tatattcaaa aacagcaaat tcatctccac
     2461 caagtcttcc aataatagca tcatttacga atactttagt caaaatattt gaaaaatgta
     2521 ttagtatttc atctcctcga acatgaccaa agatatcatt tacctgttta aaattatcta
     2581 aatcaaagat gtaaaagtat ccatgtgaaa cagttttaag catttctttt atgtgtactt
     2641 caaaaaaatg tctattgtat agtccagtca taaggtcttt ttctgatttt agtttaaatt
     2701 catttgccat tgctagtgct tcttgtgctt tttcagttat tgttttaggg taatactcat
     2761 ctttcatttg gtcaaagttt ggaatagaat ggtgaacatg gataagttta atcatatcat
     2821 ctgtaactct gtataccata gtaaatcgag tatccatttc tacatagata tctttttcta
     2881 ggtaatcttt ttctattacc caaagagtac catatactat aaaagcttcg tctgtgactt
     2941 tggtacatga ataccactca tctataatct caaagtctat ttgctctgat tctgtttggt
     3001 ctttttcaag acctataata aattcttcta atgtttcgta aaattcatgt tttcctgttc
     3061 caattaatgc aaggttgtca tctaataagg aaataataaa tttaaggttt tcttcttctg
     3121 gatttaaaat ataaatatgc caaatttttt ttgataaatc tcttaattgt ttttgtattg
     3181 tattattttc catagattac ccccttaaga tttacagttt atattttaat gtattataag
     3241 ttgaactctt attacaatat tattataata tagaactaaa gaaattacat cctgtatagg
     3301 taaaagtata tcttttacaa taaaaatatt acagtaaaat gaccagtttt aatatacgta
     3361 ttatattttt aatttataaa cgaatattat aaatgaaatt taaattcaac cttgaagaaa
     3421 taagctaaat gtattagtat attaatccat gcttgaacat aaataacctt tatgatatga
     3481 tatcaataat aatattatga gaggagtgcc ttgatgtcgg agttatataa tggtaaccaa
     3541 caattaaata gttggagaca catatattct gtataatcag aaatttcaag tgtattttta
     3601 atacatttaa tttttgtgtc ttattctgcc tttatagata actgacatat gacaacaaat
     3661 agtatttaaa atatatatta ctttgttaaa ttatggtgta gtaggcttta atctgctaca
     3721 cttttattaa ttttgtatag tattattttg ataactattt tgaaatagag agcattaatt
     3781 tttctagtcc acaggagttt tctgtttttg tgctgttaaa tataagcaca gaaaaaggca
     3841 gatatatacc tgtggacttt ttttatataa attatgaaat atggaggtag ttaaaatgag
     3901 tataattgaa cttaatgaac tgactaaagc ttatagaaga tttgagaaaa aggcaggatt
     3961 tatggggagt gtaaagtctt tatttaatag gaaatataca gaaaaaacag ctatattgtc
     4021 atttgattta aagatagaag aaggagaatt tgttggatta ataggtcaaa atggagctgg
     4081 taaaactact cttattaaga tgttaactgg tattattgca ccgtcttctg gagaaatatc
     4141 tgtaatgggt tattacccta acaaattaga aaataatttt aaaaaacaat atgctgttgt
     4201 tatgggccaa aaaagtcaac tattctttga attgacacca gcagatacgt ttcttttatt
     4261 taaagaatta tatgatatat ctaatgaaga atataaaaaa aacttggatt actttatcga
     4321 attatttgag gtatctgact atttaaatgt tcaagttagg acgctttctt taggagaaag
     4381 aatgaaaatg gaactaatag tagcccttct acataatcca aaaatattat ttttggatga
     4441 accaactata ggcttagatg ctatagccca aaaacaaatt aggtatttcc taaaagaagt
     4501 aaatgaaaca aaaggaacta ctattatact tacttctcat tacatggaag atattaagag
     4561 tctctgtagt agaagtattg ttgtagaaaa tagccgtaaa atatatgatg gttctacaga
     4621 gaaactattt aatcattttc aaaaatataa aaaaataaca gttagttttt ccaatgaaac
     4681 taacattgta tttccaaaag attgtaaagt tgttgaacaa acaaatcata agatatcagt
     4741 aattgtgcca aaagaaagag gaaatgaaac gattaaatgg atttttgata aatatgatgt
     4801 taaagatgta atgattgaag aagaagatat aggtagtatt gtagagagaa tatataagaa
     4861 aggaagtgat gagtattatg agcaactata atgaagcaag ttcaaatgat tcaatgtttt
     4921 ataaatttaa acagagtatt agaaggtatt atgaagtagc caaaattaac tttaaagtgc
     4981 aacttatctg gagatttgat gtggtggtta atatggtgtt tacgattgta aaaattttat
     5041 ttgcttatat tttatggaaa gcaatttttg gtgaaaataa aatagtctct ggttttacat
     5101 ttaactctat gttgtcttac tatataataa gctcattttt agcaggtatt gatatgtcaa
     5161 agagtaccag tgaagagata tgtaatcgtg taaaattggg caccttttca aagtacatga
     5221 ttattccaat taatgttcaa aattatttta ttgctaaaaa ttttggaatg acatgttttt
     5281 attttgtatt taatatgatt tctgcatttg tatggatttt tatatttaaa atagattttg
     5341 cctttacatc aaatccagtt caaatattaa tttcaatttt gatgattttt ctagggttta
     5401 tttttatgat gaaactccac ttctttattg ggattttgtc atttaaattt cttgagatta
     5461 gtttttttaa aatgataatt gaaaatattg ttgaatttat tacaggaaca atgataccac
     5521 ttgtactctt accagaaact atagtaacag ttatgaaggt atttccattt tattatacaa
     5581 catatttgcc atctatgtta ctaataggta gaaacagcaa tgaggcaata caaggattaa
     5641 ttattctagc attatgggtt ttattttttt ctgtagtaaa taaagttatg tataaaagaa
     5701 tgagaacatt atttgatggg gtgggtatat gatgaaaaaa aatttgaaaa taatatttat
     5761 gttattaaaa atgaaattat caaagatgat ggtatttaga ttcgattttt ttggagcatt
     5821 ttttgtagat agttcattat ttatattaca acttctaatg tttaatagta tatattctca
     5881 tgtagactca atagggggat ggcaacaagg agagatgtta atatttatag gaactttctc
     5941 attaataaat gcgataaata tgacgatttt cttttttggt atttatgata ttccacgaaa
     6001 aatccaagaa ggagaattag actactatat tacaaaacca ttagacccac tatttaggtt
     6061 aacatttgaa aatattaata ttggttcatc accattaatt ctagcaagta tacttattgt
     6121 tttatatgga atatctgagt taaactcaga gataacttta gttatgataa taatatatat
     6181 tttcatggtg ttattgatga cattactttt ttatgatgta tgtatcatac ttagaacagt
     6241 ttcatttttt gttatatcat cttctgcgat aatgagatta gaagataatt tattgccaat
     6301 gaatatgaaa attcctggtg taatatatga aggtgttttt aaagtattat tctatttaat
     6361 cttaccatat ggaattatgt cgacaattcc gactcaaatt ttggctggga caataacaat
     6421 taatggatta atatactcaa ttttaattgt atttttgttt acattgttta tgtggagatt
     6481 ttggaagtta ggtatgagac attataaaag tgcaagtagt taataaatta gtaaaaatag
     6541 acaatgtata actatatata aatacgtttc atatgacact aaactatgtg aacaagtatt
     6601 tgcgctaaag gcactgaaag ctataaaatt attaaagtaa ggatttaaaa gtttctttag
     6661 tagaaatata attgtgtaaa aaagcaagat taattaaaac tatatccaaa taattagata
     6721 taattcttat gcagaaagga ggtctgtaaa tgtctaattg tttggaatat attttaaaaa
     6781 taattaatgt acagaaaaat aaacaaaatg agtgatgtat attattcaga aaatcatgaa
     6841 caagataatc tttatgagca agataattta ctggcacaaa gatttaattt atttactcta
     6901 ttgaagtttg catttcctac tatttttatg atgattttta tggggttgta tacaatcgta
     6961 gatactatat ttgtatcaag atttattaat acaaatgctc tatcagcaat taatattgta
     7021 tgtccaatta ttaatcttat agttggaatt ggaactatgc ttgccactgg aggaagtgct
     7081 attgtagcac gtaagatggg aaatggtgat attgagggag caaataaagc tttttcttta
     7141 atcgtattat caggagttgt aattggggta ataattactg gtataggtat tgtatttatg
     7201 gattatatca ttatatggtt aggagcaagt gaagttttaa ttaaatattg tcgttcatat
     7261 ctttttatta tcttggtttt tgcttctgca aatatactac aagtattatt tcaatgttta
     7321 tttgtaacag ctggaaagcc tcaatttgga ttatggctag tgttaggtgc tgggtgtgct
     7381 aatataattc ttgactatgt gttcattgtt ctgttacaaa tgggtattag tggagcagca
     7441 cttgcaactg gagtaggata cctaattcct accgtttcag gtatggtttt cttttcaaaa
     7501 aataaagggt tacttaaatt ttgtatacct agactagata aaagaatatt atttgaagct
     7561 tgttttaatg gttcatctga aatggtgggt caactatcaa cagcagttac tactttttta
     7621 tttaatgcta tgatgatgaa attaattggt gaagatgggg ttgcagctat tacaataatt
     7681 atttattctc aatttctttt aacttcgctt tatataggtt tctcaatggg tgttgcacca
     7741 ataataagtt ataactatgg aagtgaaaat aatacgcagt taaatcatgt ttttaagatt
     7801 tgtatattat ttatttcagt agcatcttta ttggtattta tcttttctat atcatgttca
     7861 ccaatcattg tagagatttt ttctcaaaga ggaagccgag tttatgaaat tgcaaggtct
     7921 ggattttata tattttcttt tagttttatt ttttgtgggt ataatatttt tacttcgtct
     7981 atgtttacag cattatcaaa tggaaaggtg tctgcaatat tatcttttct acgtacattt
     8041 ggatttataa ctataggttt attgatattg ccaaatatat taaatataaa tgggatatgg
     8101 ttagcagtac caagtgctga gattttaact ttatttttaa ccattttttg tatatgtaag
     8161 tataagaata gatataaata tatgtagata ttaaataaat agtttaggta gatattttat
     8221 gttttaataa gtaattcaat tagtgggaga tatatactat tgaaaattaa agttataatt
     8281 gatataaaga attaataaaa ataacatata aaagtttaaa agggagttga acttaaagtt
     8341 tgattagaaa aagtcaattt taagacaact ccttttattt tactattgta ttaatctgta
     8401 tctgtaagtg tattagtcta atattataaa ctttggatga aattaatttt ctataggttt
     8461 taatgtatca tacactttat tttaaagaaa cttatcttaa ttttcatagt gattgaattt
     8521 gtctattaga gatgttacta ttagtaatgt aaaagcaaga gtagtgaata taaaaaacaa
     8581 gatagtgata ctgcaagatt agaatataat aaatttttat ggagagattt caatatggat
     8641 acatgtttta ataaaaaaat attacttgtt gatgatgaaa aggatatagt agatttaatt
     8701 gaagaagtac tcataaatga tggttttaaa aacattatta aagcttataa tggtctagat
     8761 gctatttcgc tgtgtaagat agcatgtcca gatgttgtca ttcttgatat aatgttacca
     8821 gatatagatg gtatagaagt gtgtaaaaaa attcgagagt tttcatactg ttctattctt
     8881 tttttatcat caaaaaatga tgatattgac aaaatactag gtcttagtag tgggggagat
     8941 gactatataa caaagccatt cagtccaaga gaaattgctt ttcgagttaa agcgcaatta
     9001 cgtagacaac agtatcaaag cattgtacca tcagatagtg aagttattaa aataggagat
     9061 attactattg atatagaagg aaaccgtgta tataaagata gaaatgagat tgagttaact
     9121 ggaagggagt atcatctttt aagctacatg gctcaaaatg taaataaaat tataggaaaa
     9181 gaaagactct atgaacaagt atggggagta tatagtagta tttgtgacaa tacaattatg
     9241 gtgcatatac gacatataag agaaaaaatt gaagataacc cctcaaatcc taaaatactt
     9301 ataacagtta aaggactagg atataaactt gtgaatagaa tcgattagaa tagattttaa
     9361 tattaatgaa gatatctcaa gttgaatttt tagttaatga ggcattcttt tatgggtaaa
     9421 atcaaatata ttttcgttat ctcaacaatg aagaagaggt ctatctctat tttgagatag
     9481 cctcattaag tttatatcct ggtaatgtac tttatcttct aataaaattt ttttagttta
     9541 tttgcagtga aacattaact agaacattaa aatttgcatt gaaatactaa ttaaataata
     9601 ttgtatattg tgtataaact tatactaatt gtttttctat attaaatact ttatcaatcc
     9661 aacctttata aaatttttct tcatgggaaa ctaggattat acttcctcta aactgtttca
     9721 aagcattttg taaagcatcc tttgtctctg catcaaagtg attggttggt tcatccagga
     9781 taagaaaatt acaaggagat aacatcatac aacataattt aaccttagat tgttcaccac
     9841 cacttaaagt agatacactc cttgaaacat gctcttcctt cacaccacat ctggctagat
     9901 gacgtctaat ctcttttgta tttaattttg gatatttatc tgctactatt tgaagtgggg
     9961 ttttatcagg attttcccat tttaaatctt gctcataata accaatttta acttgttctg
    10021 aaaactggaa atctccagat atacgaggaa tatctttgac taatgtcttt aacaaggtag
    10081 atttaccgat tccattaaaa ccagttataa ctattttttg acctccatca acagagaaat
    10141 ttaattttgg aagtaaagaa tagtagtatc caacttcaag atttgttata gtaagagcat
    10201 tttgagccga tatttcaatt tctgaaaatt gaatatttgg ttttcctgta aaactaggag
    10261 gagctattct ttctatttta tccaattgtt tttgtcttcc tcttgcaatt tttgaattta
    10321 ctccagcttt atttttacgt ataaatgttt cttctttttc tattttcttt tgttgtgctt
    10381 gataccttct aatataatct tctcttaaat gtgatttttg tttaaggaac tctgaatatt
    10441 ttccatgata tttcttaatc atgccaaatt ctatatcaag aatacctgta gaaatttttt
    10501 ctaaaaagtc aaaatcgtgg gaaactacaa taaatgcccc attaaatgta tttaaataat
    10561 ttgagagcca ttcaacatgt tctttatcta aaaagtttgt tggttcatct aataatagaa
    10621 catcatggtt agaaagtagt aattttgcta aaattacctt tgccctttga ccaccactaa
    10681 gctctccaac aacacgattc attcctattg aatctaatcc aagaccattt gctattttat
    10741 ttatttcatt atctatagaa tagaaattac ttgctattag ttgttcctga taatctgatg
    10801 ctttgattag atattgctca ttttcagata tagcactttt ttgatagagt aaattcattt
    10861 ccttttctat tttataaagt tcttcaaatg cagtgtgtaa atagagtgat attgttgtat
    10921 ctctattgat ttctgcatat tggtctaaat ggcctatatt gatattaggt tgccacttga
    10981 tttctcctga atctggaaca acttctccca ataaaatttt aattaatgta cttttacctg
    11041 taccattttg gccaactaca cctatatgtt cacctttgaa taaatcaaag ttagctttct
    11101 catacagtga tttatccata aaacattgtg ataaattagt tacttttaat aaactcataa
    11161 ttcaaactcc ttttctttta ttaaaaaatt tatggaaata aaaaaagcag aggaatggca
    11221 ttgtaaaatg ccgacctctg cttttggaaa aacaaaaggt catagacaag cattgttgtc
    11281 tatgaccaga aaatctatat tatctatcgg caaataggta attttaaata agcatagcca
    11341 aaaaactata ctaaacctat atttgccgat gaagatagaa agctttaagc tttcagatta
    11401 acctctacac aacgcttcat cgtatacatt tgtgtagagt taagttttct aacaagttta
    11461 actaattcta tataaatcat aattattcac atcctaatct tagtattaat tctagtttat
    11521 ctgtatttaa attgtacttt tgtactttgt ggattatctt atttatttaa aatatgtttt
    11581 attaattata gcttaagtta tgttatcata atacttagtt atagtcaata agaaagttat
    11641 atttactatt attatattaa tttagtaaat ctatcattac aaagttattt gaagactcgc
    11701 tatcaataaa attttatagt ctataagaaa attaagatta tgagttttat aattttgata
    11761 tagggatact attttcataa atattacaac atgtagaagt atatgtctat tattgttgaa
    11821 ttttcaatga tatattaagg ttctaatata ttatacaaaa taaaaacatt aattcaactt
    11881 aaataatttt aagtattata tgtgttttga ttaaagaaaa ctaaaatttt aaggaaaaat
    11941 aacatatttg tattaaattt tattgaaatg attaaattta tttacttaat gtaaattatt
    12001 agctaaaatt gtattaaata attaaggagg tgagtagctc tggatgaaat agatttaaaa
    12061 ataattaatt ctctaaaaaa gaatagtagg gcttcaacct ctgaaataag cagacaaatt
    12121 aatttatcag taccagcagt ctcagagcgt ataaggaaaa ttgagcagtc aaatttaatt
    12181 caaaagtaca ctattcaaat taatcgggaa aaaacaaaat ataagctttt ggcttttata
    12241 tttatcaata ctgatttatc taaaagtatg aaagaattta agaaaactat attggaatat
    12301 gatactgtat tagaatgtca tcatatttca ggagagtatg attacttaat aaaagtatta
    12361 gcagaagata caagtaatct tgaagagttt atatcagtga ctttgaagga aattgggggg
    12421 attcaaaaag ctaatacaac cattgtttta tctacaatta aagaagaatt taatacaata
    12481 aaagatattt tttaaataat ttactagtaa agtgttaatt atattttagg aggaaaatag
    12541 aatatgaaag ttttagtttt tttagcaaaa ggttttgaaa ctatggaatt tagtgtattt
    12601 gtagatgtta tgggatgggc tcgtaacgat tatgggcatg atattgatgt agtgacttgt
    12661 ggatttaaaa aacaagttat gagcacattt aatatacaag tattagttga taagacgata
    12721 gaagaagtat gtgttgatga ttatgatgct ctagctattc ctgggggatt tgaagaattt
    12781 ggattttatg atgaagcata tgattcatct tttttaaatc taataaggga gtttaattca
    12841 aaagaaaaaa taatcgcttc tatttgtgtg gcagcattgc cagttggaaa aagtggtgtt
    12901 ttaaaaaatc gtaaagctac tacatatcat ttgaaaaatg gtaaaagaca aagacagtta
    12961 agtgagtttg atgtaaatgt agttaatgaa ccaattgttg tagataaaaa tattataacc
    13021 tcatattgtc cagaaacagc tccacatgta gcttttaagc ttttagaaat gttgacatct
    13081 aaagaacaaa tggatgaagt taaattagca atgggattta aactgtaatg gtttatagat
    13141 tttctaagta atcagaaccc cagtcacaca tggaatctaa aattggaata atactctttc
    13201 caaaatcaga tagcgagtat tctacttttg gaggaacaac aggataaacc actcttataa
    13261 ttaaaccatc tgattctaat tcacgtaatt gttgagttaa catcttaggc gttgctttag
    13321 gaattaattt ttttaattca ttgaacctca atatagtgtc tttaagatgc caaagtataa
    13381 gtgtcttata tttaccacca atcaatgcaa gtgttgcttc tattgggcaa ctgtaattat
    13441 ctaaacattt tttcattttt gggctccttt atttatttta aagtatcttt ttaggtacta
    13501 tattacaaaa aagtgcatta ttgtaatata gtaccatagt gataaaatta tagcataggt
    13561 taaataaatc atcaacttag cctaataaaa caataggagg taaataaatg aatttcgttg
    13621 aacttgcaaa aaaaagatat tcttgccgta attatcaaga tagaaaggtt gaaaaagaaa
    13681 aacttgagaa ggttttagat gttgcaagaa tagctccaac tggtggtaat cgtcaacctc
    13741 aaagattgat tgttattcag gaaaaagaag gtataaataa actttctaag gctgccaata
    13801 tatatgatgc tccattagca attcttgtat gtggagataa agacaaagtt tggacaagac
    13861 cttttgatgg gaaacaactt acagatattg acacaagtat agttacagac catatgatgt
    13921 tacaggcaac tgaacttgga ttggcaagtg tttgggtgtg ctattttaat ccagatataa
    13981 ttagagaaga gtttagtttg ccagataatc tagaaccaat aaatatatta ttgatgggat
    14041 atgaatctaa gataccagag agcccagaga gacatgaaaa aactcgtgtt cctttaagtg
    14101 aaattgtttc ttatgaaact ctttaattat aaaattaagt tattcatagg ataaatgtaa
    14161 taaagtgttt attataaaca aaattcaaaa taaataatat ctacatataa ttaattttag
    14221 tgaagtatat ctagtttaac tagaatgtat acttcactaa tttatttatt caaaatattt
    14281 atgaggattt taatatatta actaaaatat atcaattgaa ttaactttta aatctggaaa
    14341 agcacttgaa atatagtgtt ttatgtcatt taaatggtct tgaatttgct tttctgatat
    14401 tttatcaggt ccatatacaa aatacataat atttttagta tcttttgtta tagctgttcg
    14461 attatcaggg ccattaagaa tactagataa tataccaata ttatctgata aaaataaatc
    14521 atcttttgtt aaaatatgtt ctttaccacc tataccataa aaatgttcac ttccatttgc
    14581 taattctatt ttcaaaggta attccatttt atcaaggtca tgacctgctg ttagaagaag
    14641 atttttcaat tctgctaaaa acattgactc aattgttaca cctacatctg ggataccttt
    14701 tgattttaat agaatagatt ctaattgaag caatacaggg taagttttct tgaatttttt
    14761 ataatacttt atataacttg aaacaggctc tgttttaatt ttttctttac gattgtattc
    14821 tgggtgttgt tctataatat ttttaattgt agagttttta agaactaaaa aattttcttt
    14881 attaggtttt gaatgtgtaa tatttataac cataactcca aatttaatat tgggataaat
    14941 ttctttaact ttatgtgata tttctaacat aatatagact cctttttatt ttatatttta
    15001 tgatataatc ttagcaaatt aaaagtggat tttgtagttc catttttata gtaattttat
    15061 agaaccactt ttggaggtat tagaatgtgg attacgatta atagaaatag taatatatca
    15121 ttatcaagac aaatttattg tgagataaaa gatatgattc tggatggaag cttaaagttt
    15181 gagcaaaaac tgccatcatc taggacttta tcaaaagaac tatctgtatc tcgtaataca
    15241 gttttagatg catataatca attaattgca gaaggatatt tggaaacaag gaatggatat
    15301 ggaacaattg tagctaaagg aatatcaggg cataaagttg attttaagtt aaatgatact
    15361 tttagagatg aaaaagattt acatactaat aaacaaaaaa attatataga ttttcgttca
    15421 ggagttccag aattaaaact atttccacgc aaagaacttt caaagctata taaacaaata
    15481 ctgtatgatt tatcagattc ggatttcaga tatggaagta cagctggtgt atgggtttta
    15541 agagtggaaa tatctaagta tttatttcgt actagaggaa ttagatgtaa tcccaaaaac
    15601 attatgattg tttctggttc aacacaaggt ttatctttaa tttcacagct tttatacaaa
    15661 agtaataaaa aggttgctgt agaagaccct attcataaag gcttattaaa ggttatttca
    15721 tctattggat attcagttgt tggaattaaa gtagatgata agggaatgaa taccaactta
    15781 ataaaatcaa taaaggggat ttcttttgtt tatactacac cttctcatca atacccatta
    15841 ggagggattt tacctattca aagaagacta gagcttatta aatgtgcaac agagaatgat
    15901 tactatataa ttgaagatga ttatgatagt gaatttagat atgagggtca accaattagt
    15961 tctctttatg aacttagtcc aaataaagta atttacatag gttcttttag taaaatatta
    16021 tctccagcta ttcgtgttgg atttatgtta ttaccagaca atattttaga aagctataaa
    16081 accttaaaaa tgtactctga tgttcataca gaaacaatat tgcaacatgt attagctgaa
    16141 tttattggaa atggaggttt agagaagtac atatttagaa tgaagaagtt gtacaataga
    16201 aaacgtaagt acctcataaa agttttaaac acggaattta aaaatgaatt taaaataaaa
    16261 ggtgaagcag cagggcttca tatagttgct catttctata atgttaaatt tacggaaagt
    16321 ttaattcata aaatttttga agcaggtata aaagtttatc ctatacaaca atatgcaatt
    16381 gagaatatat tagaatatga ctatgaaatt atactaggtt atgctcatct tagttttgat
    16441 gaaattgaaa aaggtgtaaa aatactcagc aaaataatac atatgaatca aggtacatag
    16501 ataaaacatg tagcaatcta catataatag atgtaattaa gtttaaactt taaaagaaat
    16561 atatattaat atttttgtat aaaaatacct gatatttttt tatcgatact ttattgctat
    16621 acactaaatt aagatagcaa tttatattaa gaaaaaactt tttgcatagg ttataatact
    16681 taaaaggaga ggatagagtt gaaaacaaat acagatgtta ttatggagat aattgattat
    16741 atagaagaaa atcttacaga aaatctaaat ttagataaaa tagcttatat tgctaattat
    16801 tcaaaatacc atttacacag aatgtttaca aatgttgtag gatgtactat ccatcaatac
    16861 atacagagga ggagacttac tgaagcagct agacaactta tatttacaga caaatatatt
    16921 atagacatag ctattacagt aggatattct acacatcaat cttttacact agcatttaga
    16981 aatttatata aagaatctcc acaagtatat agaaataaag atgaatttta tcctattcaa
    17041 cagagatttg ttttaaatct aaatcatcaa attcaaagtg aaaaatgtat ttcttatgca
    17101 aatatgaatt ataaacaaag aagagatgaa atcaattctt atctgaactt taatattaat
    17161 tgtgaggtga aagtagcatg agtttagtgc cacatgtaat tgaacaaaca ggtcaagggg
    17221 aaaggtctta tgatatatat tcaaaattat taaaagatag aattatattt ataggagaag
    17281 aaattaatga tgctattgca agtttagtag tagctcaatt actgttttta gaatcagaag
    17341 acccagattc agatataatt atatatataa atagccctgg tggctctgct acatcaggat
    17401 ttgctatata tgatactatg aattatgtca gatgtgatgt atcgactatt tgtattggta
    17461 tggctgcaag catgagtgct tttttacttg caggaggaac acatggaaaa agatgtgctt
    17521 tacccaattc agagattatg attcatcagc caatgggagg agctaaaggt caagcaacag
    17581 atgtaaaaat agctgtagat aatatattga aaataaaaga acgattagat aaaattcttt
    17641 ctgaaaatac aggtaaatca atagaagata ttaggagaga tacagataga gataatttta
    17701 tgactgcact agaggctaaa gagtacgggc tgattgatta tataatgaat aggaatgaat
    17761 aaagtatcaa agtatcatat taaaatgtct gttttacaaa tatatatata atattaaata
    17821 agagtgtctc aaaatgaact ttttagttta tgggacactc ttttttgtat gaaattaaat
    17881 atggttttat catttcgaca attattacca aatttcaagt ttaataaatt ctactataaa
    17941 aaatacataa aagataaggt tctttagtaa ctttctgtcg tcaaaagttt ttataaattt
    18001 aataacaaaa caagagaaaa taccatatat tacttatgtc tgcaatatat agaagacaaa
    18061 ggagttgtac attatgatta caataagttt gtgcatgatt gttaagaatg aggaagaagt
    18121 tataggcaga tgcttagaat gtgtaaagga tatagtagac gaaattataa ttgtagatac
    18181 cggttctaca gattccacaa aagaaatagt ctcaaaatat acagacatgg tgtacgattt
    18241 cgaatggata gatgatttct ctgctgcaag aaatttttca ttttctaaag cttctaaaga
    18301 ctatactatg tggcttgatg cagatgacat aattttagaa gctgatagag aaaaattatt
    18361 aaagattaaa gaaagcttgg atacttccat tgatattgtt atggcaaaat acaatgtatc
    18421 ctttgatgaa aatggtaacc caactctctc atattataga gaacgattgt ttaaaagaag
    18481 ttctaatcat aaatgggttg accctattca tgaagtagta cctctttttg gagaggtatt
    18541 ttattctgac attgcaatat ctcataaaaa gcttcataga caggaccctc taagaaatct
    18601 tagaattttt gaaaaaatga tttcagaagg aaaaacattg gagccaagac accaattcta
    18661 ttattctaga gagttgtact ataatgctag gtataaagaa gcaatagaag gttttactaa
    18721 gtttttagac tcatcaagag gatggattga ggattgtatc agtgcttgca gagatttagc
    18781 cacatgttat tacttaatta atgatgaaaa aagtgcctta tattcattgt ttagaagttt
    18841 cgaatttgat gagccaagag cagaaatttg ttgtgacata ggaaagcata tgtttgatag
    18901 acaaaaatat aaagaagcca ttttttggta taaggtagca ttaacaagag ataagaatga
    18961 tactgatgga ggctttaagt cagatgattg ttatggatat ataccatcta tacaattaag
    19021 tgtctgttat gacaggttag gagaatcaga taaagctatt tattatcatg aaaaaacaaa
    19081 agaaatcaaa cctaatgata gtgctgtttt gcacaatgaa aattatttta gtaaatttaa
    19141 aaatagttga tgggtcacaa tcatactaaa tttgacatat tatatataga gcttaaaagc
    19201 tcaaatatat caggaggtgc ttttaataat gagtagaaat aaatattttg gaccatttga
    19261 tgataatgat tacaacaatg gctatgataa atatgatgat tgtaacaatg gtcgtgatga
    19321 ttataatagc tgtgattgcc atcattgctg tccaccatca tgtgtaggtc caacaggccc
    19381 aatgggtcca agaggtagaa ccggcccaac aggtccaacg ggtccaacag gtccaggtgt
    19441 aggggcaaca ggcccaacag gaccaaccgg tccgactggt ccaacaggaa atacagggaa
    19501 tacaggagca acaggattaa gaggtccaac aggagcaaca ggggcaacag gcccaacagg
    19561 agcgacagga gctataggat ttggagtaac aggtccaaca ggcccaacag gagcgacagg
    19621 agcaacagga gcagatggag taacaggtcc aacaggtcca acgggagcaa caggagcaga
    19681 tggaataaca ggtccaacag gagcaacagg ggcaacagga tttggagtaa caggtccaac
    19741 aggcccaaca ggagcaacag gagtaggagt aacaggagca acaggattaa taggtccaac
    19801 aggagcaaca ggaacacctg gagcgacagg cccaacagga gcagtaggag caacaggaat
    19861 aggaataaca ggtccaacag gtccaacagg agcaacagga gcagacggag caacaggagt
    19921 aacaggtcca acaggggcaa caggggcaac aggagcagat ggagtaacag gtccaacagg
    19981 ggcaacagga gcaacaggat ttggagtgac aggcccaaca ggtccaacag gagcaacagg
    20041 aataggaata acaggagcaa cagggttaat aggtccaacc ggagcaacgg gagcaacagg
    20101 agcaacaggc ccaacaggag taacaggggc aacaggagca gcaggactaa taggaccaac
    20161 cggggcaaca ggagcaaccg gagcagatgg agcaaccgga gc
//
LOCUS       DAYAPA010000069        20157 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.069, whole genome shotgun sequence.
ACCESSION   DAYAPA010000069 DAYAPA010000000
VERSION     DAYAPA010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20157
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.069"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            <1..980
                     /locus_tag="ACGI59_002310"
     CDS             <1..980
                     /locus_tag="ACGI59_002310"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HFG9740095.1"
                     /translation="NLKLIWINILNPVFIKNEIKRKKIQIIIFFFINFGLTYSLGLLL
                     YYNKFIDPENFASFMMILPLSSVAIAKFYTEGLNNDMYEFYSLIILFFLIYSLLIVLK
                     LLNLINNNEFIIINLILTFISSLSIIIYSSNKKNLYPVKNIKLGSLLIIYFMISVIVP
                     TSFILILQGAQPNYSGIIKFIFTPITSLTTIYIFFSEEYAWRGFLQNIFFDKFGKKLG
                     VIILGMCWSLWHLPLIFTLYTPEAPILGIILRSIHIVGISIFLGYLYIKTKNIWFCAI
                     IHVLNNSAFLITNSKESTITYHDILIVSMVVLIFYVPFLFTKEYKNNLE"
     gene            complement(1079..2041)
                     /locus_tag="ACGI59_002311"
     CDS             complement(1079..2041)
                     /locus_tag="ACGI59_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HFG9740096.1"
                     /translation="MNKSWSNLIIKNKDKKVRSGWIIIAVMATFYILQYYLSMILVEI
                     IRKILVATGDINLKTNYFSEYANWLNDVGLQIVFQILTDSLMIIIPIIVWRFIMKNPI
                     SEMGLHSYRIKKKEGYVGMLLGIFNCTVIFIITITIGGGHVTSWVPKISPLTVSWILV
                     FILVAFAEEILNRGFFMAVLRRCKNIYFIMIVPSVIFGLIHIWNPDVTFLSVINIIII
                     GILFSYMFIKSSNIWMCIGYHFAWNVFQGIIYGMPVSGLQVPGIITTQITHGNLLNGG
                     MFGIEGGILTTFVNLLSFIFVWYYYRDSKYDFVSDKVNVTNIKN"
     gene            complement(2357..3307)
                     /gene="hpdA"
                     /locus_tag="ACGI59_002312"
     CDS             complement(2357..3307)
                     /gene="hpdA"
                     /locus_tag="ACGI59_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase activase"
                     /protein_id="HFG9740097.1"
                     /translation="MSSQKQLEGMIFDVQSFSVHDGPGCRTTVFLNGCPLSCKWCANP
                     ESWTVRPHMMFSELSCQYENGCTVCHGKCKNGALSFNLDNKPVIDWNICKDCESFECV
                     NSCYYNAFKLCAKPYTVDELVQVIKRDSNNWRSNGGVTFSGGEPLLQHEFLHEVLLKC
                     HEVNIHTAIETSACVSNEVFNKIFNDIDFAFIDIKHMDREKHKEQTGVYNDLILENIS
                     NLANSDWNGRLVLRVPVISGFNDSVENISDIISFMHKNNLVEINLLPFHRLGESKWIQ
                     LGKEYEYSDKGDVDEGHLEELQDIFLDNGIACYVGHETAF"
     gene            complement(3300..3557)
                     /gene="hpdC"
                     /locus_tag="ACGI59_002313"
     CDS             complement(3300..3557)
                     /gene="hpdC"
                     /locus_tag="ACGI59_002313"
                     /EC_number="4.1.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase small
                     subunit"
                     /protein_id="HFG9740098.1"
                     /translation="MRKHSDCMNFCAVDATKGICRLSKQMINLDDAACPEIKVMPKCK
                     NCKNFVEANDEGIGKCVGLEKEDWVYSTLNAITCEGHVFNE"
     gene            complement(3561..6269)
                     /gene="hpdB"
                     /locus_tag="ACGI59_002314"
     CDS             complement(3561..6269)
                     /gene="hpdB"
                     /locus_tag="ACGI59_002314"
                     /EC_number="4.1.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase large
                     subunit"
                     /protein_id="HFG9740099.1"
                     /translation="MSQSKEDKIRSILEAKNIKSNFQNKENLSEFNEKKASKRAEDLL
                     DVYYNTLSTADMEFPYWYNREYRKSDGDIPVVRRAKALKAAFSHMTPNIIPGEKIVMQ
                     KTRHYRGSFPMPWVSESFFVAQGEQMREEAKKLASNTADELTKFGSGGGNVTESFGNV
                     VSIAGKFGMRKEEVPVLVKMAKEWVGKSVEDLGFHYEKMMPDYDLKENLMSTLICMFD
                     SGYTLPQGREVINYFYPLNYGLDGIIEMAKECKKAVAGNASGDGLIGMDRLYFYEAVI
                     QVIEGLQTWILNYAKHAKYLESIETDLEAKKEYSDLVEILEHIAHKQPRTFREALQLT
                     YTIHIASVNEDAISGMSIGRFGQILYPWYEQDIEKGLITKEEVIELLELYRIKITCID
                     CFASAGVNGGVLSGNTFNTLSIGGLKEDGSTGANELEELLLEASMRCRTPQPSLTMLY
                     DEKLPEDFLMKAAECTKLGSGYPAWVNNSNGTTFMMKQFADEGMTVEEARAFALGGCL
                     ETSPGCWKQLTLNGKTYSIAGGAGQSAGSGVHFIANPKILELVLMNGKDYRMNIQVFE
                     PHNKPLDTYEEVIEVFKDYYKQAINVLERANNIELDIWRKFDTSIINSLLKPDCLDKG
                     QHIGNMGYRYNATLNVETCGTVTMVNSFAALKKLVYDDKAFTIEEMKDAILNNFGFKD
                     ALEVGNYSMADQVKVDKTGKYDAIYKACLDAPKYGNNDLYADNILKNYEVWLSKVCEE
                     AQSLYAKKMYPCQISVSTHGPQGAATLATPDGRLSGTTYSDGSVSAYAGTDKNGVYAL
                     FESATIWDQAVVQNSQMNLKLHPTTIKGQQGTKKLLDLTRSYLRKGGFHIQYNVVDSE
                     TLKDAQKNPDNYRQLMVRVAGFTQYWCELGKPIQDEVIARTEYEGV"
     gene            complement(6566..7582)
                     /gene="tsaD"
                     /locus_tag="ACGI59_002315"
     CDS             complement(6566..7582)
                     /gene="tsaD"
                     /locus_tag="ACGI59_002315"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFG9740100.1"
                     /translation="MSDIITLAIESSCDETAASVLKNGREVLSNIISTQIETHKKFGG
                     VVPEVASRKHVENIDIVVQEALDKANIGFNDIDHIAVTYGPGLVGALLVGLSYAKALA
                     YTLNIPLVGVNHIEGHLSANYIEHKDLKPPFITLIVSGGHTHLVEVKDYGKYEILGKT
                     RDDASGEAFDKISRAMNLGYPGGPIIDNLAKNGNKHAIEFPRAYLEEDSYDFSFSGLK
                     SSVLNYLNGKRMKNEEIVVEDVAASFQEAVVEVLSTKALKAVKDKGYNIITLSGGVAS
                     NSGLRAKITELAKDNGITVKYPPLILCTDNAAMIGCAGYYNFINGKTHDMSLNAVPNL
                     KINQ"
     gene            complement(7588..8064)
                     /gene="rimI"
                     /locus_tag="ACGI59_002316"
     CDS             complement(7588..8064)
                     /gene="rimI"
                     /locus_tag="ACGI59_002316"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFG9740101.1"
                     /translation="MEDNTKLIVDDIKIEEMTTEDIDEVFEVEKNCFEDYWSKESFRK
                     ELSNEVARYIVAKLNGKIVGYVGIWLILDEGHINNVAVHSDYRGKKIGDKLIKGIVDL
                     CKDNNIASMTLEVRASNKIAQNLYRKYGFKMGGIRKEYYSDNKEDAIIMWNQLKEV"
     gene            complement(8054..8770)
                     /gene="tsaB"
                     /locus_tag="ACGI59_002317"
     CDS             complement(8054..8770)
                     /gene="tsaB"
                     /locus_tag="ACGI59_002317"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFG9740102.1"
                     /translation="MKILGMDTSSMAASVAVVEDDNLICEFTVNNKKTHSQKLMPMIE
                     NMLSMSDLSIKDMDLLAVCIGPGSFTGLRIGMATVKAMAHVNNIPIIAVNSLESLANN
                     INFCDRKICCILDAQKNQVYSAKYKFENGEMIELDGVDVVEFDALVDEIVSTNEEFII
                     VGEAVYKYKDKLENIKNIKIPSPANNVSNAGSLCSLALNKYNKNIDVHTCYTINPMYI
                     RKSQAEVQYDEKMKRLNNGR"
     gene            complement(8770..9222)
                     /gene="tsaE"
                     /locus_tag="ACGI59_002318"
     CDS             complement(8770..9222)
                     /gene="tsaE"
                     /locus_tag="ACGI59_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFG9740103.1"
                     /translation="MAKIYLENENKTREIGYKLGKLLKEGSVICLVGDLGAGKTTMTQ
                     SLADSLGIEDYITSPTFTIINEYEGKIPLYHFDVYRIGSSDEMYDIGYDEYVNSNGIC
                     IIEWANLIEDILPKEYLNIELRYKDEGREMILTPKGEFYKEIVEELIK"
     gene            complement(9361..10401)
                     /locus_tag="ACGI59_002319"
     CDS             complement(9361..10401)
                     /locus_tag="ACGI59_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HFG9740104.1"
                     /translation="MHLSKKIKKLFLDDKGYYFNSNWKDIHIGIYDTKLGDSLKYITN
                     EYIENMERVKYDYRELKNIENQTDGMCILAIRQNYKALEYVKNQTEELCLEAIKYNYK
                     ALEYVKEQTEYLCLEAIKKDCNALKYVRNKTEGLIIKAISHSSNIDVVSILKALETQT
                     RRICLEAIKKDGRCLAYVREQSEELCIEAIKQNYKALKYVKNQTEKMCIESVRQNGMA
                     LQYVNKQTDKICIEAVKQDGRSIQFVNNKTEEICINAIRYLNKKYNIKDVLSYIDKYT
                     EDICIEIVRQNGKMLMYIKNQTEKMCIEAVKENYKSLKYVKEQSERICKEALKQNHKA
                     KEYVKIAIDDCI"
     gene            complement(10730..11335)
                     /locus_tag="ACGI59_002320"
     CDS             complement(10730..11335)
                     /locus_tag="ACGI59_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFG9740105.1"
                     /translation="MQNTAKRGNMISTRTLVVMSILSAISYLLMFIQAPIPGIFPDFL
                     KIDLSDIPAIFGGMSLGPLVGFGIVVVKNVLQAITATTTGGIGEFANILIGGSYVMIL
                     CYSYKRSGDLKGVLIGFVLGTISMTIIGSLVNYFVMLPLYGQLMGLDAIIGLGSAINP
                     QVHDLFTFVIWMIAPFNVLKAVIISLVTLPLYKKMGNIIKK"
     regulatory      complement(11460..11577)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(11637..12803)
                     /locus_tag="ACGI59_002321"
     CDS             complement(11637..12803)
                     /locus_tag="ACGI59_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HFG9740106.1"
                     /translation="MIFIKNGKINTITNGIIHGDILIDEGKIIEIGEDLIAPLDVEVI
                     DASNKLVFPGFIDAHTHLGLWEDGIGFEGADGNEETDPITPQLNPIDGINPMDRTFKE
                     AFEGGITSVCTTPGSANVMGGQCIAIKTCGKRIDKMVIKNPVASKIAFGENPKSCYGQ
                     DDKSPQTRMAIAALLRENLKKAEEYLEDIDMYESHDDEDCEKPEYDIKMESLIPVLRR
                     EIPFKAHAHRADDMFTAIRIAKEFNLKLTLDHCTEGHLIVDELVEEGFPVIVGPSLSE
                     RSKFELRNLTFNTAGILSNAGLDVCIMTDHPVIPVQYLPICAGIAVKHGMKEEKAIES
                     ITINPAKALGIEDRVGSIEVGKDADLVIWDNSPLEIQSNVLYTIINGKVVYEKK"
     gene            complement(12951..15092)
                     /locus_tag="ACGI59_002322"
     CDS             complement(12951..15092)
                     /locus_tag="ACGI59_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFG9740107.1"
                     /translation="MDINQILKKEFNLRDEQINNTLKLIDEGNTIPFIARYRKEMTGE
                     MSDVTLREFYEKLMYLRNLQSRKDDVVRLIDEQGKLTDEITQNIEKAKTLQEVEDIYA
                     PYKQKKRTRATIAKEKGLENLALSILENNLDNIEIEAKNYLDEEKEVLSIEDALKGAR
                     DIIAELVSDDAKIRKYIRELALREGMIVSKSATDEKSVYDMYYDYSEAVKSMAPHRVL
                     AINRGEKESFLKVKLEINNDKVLNYIINEYVNDKNFKNKEEIVSSIEDSYKRLIFPSI
                     EREIRNHLTEIAQERAISVFGKNVKSLLLQPPVKDKVVMGFDPAFRTGCKIAVVDKNG
                     KLLDYTTVYPTDPQNDVEGAKKVLKGLIEKYDIDIISIGNGTASRESETFVSEMIKEI
                     DSEVQYVIVSEAGASVYSASELANEEHPDINVSIRGAISIARRLQDPLAELVKIDPKS
                     IGVGQYQHDLNKKRLEEVLDGVVEDSVNSVGVDLNTASYSLLEHVAGISKAIAKNIIA
                     YREENGDFTSRAQLKKVKRLGPQAFTQCAGFMRILEGKNPLDNTGVHPESYDICKKMI
                     EIIGYSLDDVKNKNIGEIDEKIKEIGLRELSEKLEVGQVTLKDIIAEIKKPGRDPREE
                     GIKPILRTDVLKIEDIQEGMTLKGTIRNVVDFGAFVDIGIKNDGLVHKSEMSNSFVKD
                     PMSIVTVGDIVDVKVIGIDLNKKRVALSMKK"
     gene            complement(15236..16343)
                     /gene="prfB"
                     /locus_tag="ACGI59_002323"
     CDS             complement(join(15236..16267,16269..16343))
                     /gene="prfB"
                     /locus_tag="ACGI59_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_153309863.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFG9740108.1"
                     /translation="MLKLQEYRHSMEQMASNLEELRVSLDIASLMKNIQINEEKMNQQ
                     DFWNDNEVAQRVLQENKSLKETLEEYESLKSLLEDIEVLIEIGLEEDDDSVERDIEKS
                     IESMEEKLSEMKIKTLLNGEYDKNNAILSINAGTGGLDAQDWAQMLLRMYIRWSESKG
                     YKVKLLDIISDPEAGIKTATILVEGTNAYGYLKSEKGVHRLVRISPFDPSGKRHTSFA
                     SIDVTPELDENIEVEINPSDLKIDTYRASGAGGQHVNTTDSAVRITHIPTGVVVQCQN
                     ERSQHLNKDRAMRLLMAKLIELKELEQKEKIEDIQGKYSQITWGSQIRSYVFQPYKLV
                     KDHRTNAEFGNVDSVMNGNIDLFINEYLKMNKIV"
     gene            complement(16358..19033)
                     /gene="secA"
                     /locus_tag="ACGI59_002324"
     CDS             complement(16358..19033)
                     /gene="secA"
                     /locus_tag="ACGI59_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFG9740109.1"
                     /translation="MSFMDNLFNMADKKELKKFNKTVDIIDSLEPKFESMADSELKNM
                     TNIFKERLANGESIDDILPEAFAVVREVSKRVLGLRHYRVQMIGGIVLHQGRIAEMKT
                     GEGKTLVATAPVYLNALTGKGVHVVTVNDYLAKRDRDQMAKIYEFLGMSVGVIIHGQN
                     PKVRKEQYDCDITYGTNNEYGFDYLKDNMVIHKEQRVQRGLNYAIVDEVDSILIDEAR
                     TPLIISGPGDKSTHLYSDANTFVLTLKPDDYELEEKDKAVSLTASGIQKAEVYFNVDN
                     ITDISHTELYHHINQALRAHVIMKKDVDYVAKDGEIVIVDEFTGRLMFGRRYSEGLHQ
                     AIEAKEGLKIQRESKTLATVTFQNYFRMYKKLSGMTGTAKTEEEEFKAIYKMDVFQVP
                     TNKLMIREDLPDCVYKSEIGKFNAVAQEIIERHKVNQPILVGTVSIEKSELLSQILKK
                     KGIKHEVLNAKHHDKEAEIIAQAGRLGAVTIATNMAGRGTDIVLGGNPDFLTKREMRR
                     NGFKEEIVNRVDTPIEGIPVKGNEILFEAREEYEKLFEKFKQQTQEEQKQVVEAGGLA
                     IIGTERHESRRIDNQLRGRAGRQGDPGSSRFYIGLDDDLMRLFGSDRISGIVDKIGLE
                     EDMPIEHRILSKSIEGAQKKVEGKNFGIRKHVLQYDDVMNKQREIIYAERKRVLEGED
                     LQEQIQSMTHSIIEEAVTLYTQDKGFDEEGFKEHMYNLFLPKGSIEIPEIEKLNPVEI
                     TEKVYEIAMKIYTSKEEQVGYERMREVERVILLQAVDNHWIDHIDAMDQLRQGIGLRA
                     VGQQDPVIAYKMEGFDMFDEMNKHIKEDTVRYLFNITIETPVERKAVVDVENLSSPSD
                     GTLPTSKTVKKDEKVGRNDLCPCGSGKKYKNCCGR"
     gene            complement(19165..19716)
                     /gene="raiA"
                     /locus_tag="ACGI59_002325"
     CDS             complement(19165..19716)
                     /gene="raiA"
                     /locus_tag="ACGI59_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFG9740110.1"
                     /translation="MNIIISGKQIELTDAIKNKIETKLSKLDNYIHPDTDVKVTVSAR
                     KARQKIEVTIATVNGPIIRAEDMQENLYTAIDVVYDKLSKQLKKYKKRLQDKHKDNKS
                     IRFQDAEINLADEQENYGDENQELVIQRRKKFSVKPMSEEEAVLQMELIGHDFYMFKN
                     IDSDEIAVVYKRNNGGYGIIEHE"
BASE COUNT         6116 a         3747 c         2122 g         8172 t
ORIGIN      
        1 tgaacttaaa attaatatgg ataaatattc taaacccagt atttattaaa aatgaaataa
       61 aaagaaaaaa aatacaaatt attatctttt tctttataaa ctttggttta acttattcat
      121 taggtttact gttatattat aataaattta ttgatccaga aaactttgca tcattcatga
      181 tgatattacc tctatcttca gttgccattg caaagtttta tactgaagga ttaaataatg
      241 atatgtatga attttattca ctaatcatat tattttttct tatttattca ttattaattg
      301 ttcttaagtt attaaatcta ataaataata atgaatttat aattataaac ctaatattaa
      361 cttttataag tagcttatca attataatat attcatcaaa taaaaaaaat ttatatcctg
      421 taaaaaatat taaactaggt tctttattaa ttatttactt tatgatttct gtaattgttc
      481 caacatcatt tatactaata ctacaagggg ctcaacctaa ttatagtggt atcataaaat
      541 ttatatttac tccaataaca tcattaacta ctatttatat tttttttagt gaagaatatg
      601 cctggagagg ttttttacag aatatttttt ttgataaatt tggtaaaaag ttaggagtta
      661 taatattagg tatgtgttgg agcttgtggc acttaccact aatatttaca ttatatacac
      721 ctgaagcgcc aatattagga ataatattac gttcaataca tatagttgga atttcaatat
      781 ttttaggata tctttatata aaaactaaga atatctggtt ttgtgctata attcatgttt
      841 taaataatag tgcttttctt atcacaaatt ctaaagaatc aactatcact taccatgata
      901 tactaatagt atctatggtt gttttaattt tttatgttcc atttctattt acaaaagaat
      961 ataaaaataa cttggagtaa atagaatatc caagttattt ttatattttt gcaaatttcc
     1021 aacaatccta cagaatactt taagcttaga agatattatt aaaatttatt ataatcaact
     1081 aattcttgat attggtcaca tttactttat cactaacaaa atcatactta gaatctctat
     1141 agtaatacca tacaaatata aagctaagta gattaacaaa agtagtcaaa attccacctt
     1201 caataccaaa cattcctcca tttaatagat taccatgagt gatttgtgta gttataatcc
     1261 ctggaacttg aagtccactt acaggcatac catatataat cccctgaaat acattccatg
     1321 caaaatgata acctatacac atccatatat tcgacgattt tataaacata tatgaaaaaa
     1381 gaatcccaat aattataata ttaattacag acaaaaaagt aacatcagga ttccatatat
     1441 gaatcaaccc aaaaataaca gatggaacaa tcattataaa ataaatattt ttacatcttc
     1501 gtaaaactgc cataaaaaaa ccacgattta aaatttcttc tgcaaaagca actaaaataa
     1561 agaccagtat ccaagaaact gttaaaggtg aaatttttgg tacccatgaa gtaacatgac
     1621 caccaccaat tgttattgtt ataataaaga ttactgtaca attaaatatt cctaataaca
     1681 ttcctacata tccttctttt ttcttaattc tataagaatg taatcccatt tcactaatag
     1741 gatttttcat aataaatctc catacaatta taggaataat aatcataagt gaatctgtta
     1801 gaatttgaaa tacaatctgt aacccaacat catttaacca attagcatat tctgaaaaat
     1861 agtttgtttt taaattaatg tcacctgtag caaccaggat ttttcttatt atttccacaa
     1921 gtatcatgct taaataatac tgaagtatgt aaaaggtagc cataacagct ataataatcc
     1981 accctgaacg tacttttttg tctttattct taataatcaa gttgctccat gatttgttca
     2041 tatagatgtc ttcctcctaa ctattatttt tagttgtttc taaaatatat tctattaaat
     2101 tttttcatag tagtatttta ccacaaaact tctgcttcaa tgaagtaagc gtaatatatt
     2161 ctctaactgt aatttaaact ataaaaaata tataaagtat atttaaagca atatttatgg
     2221 acataaagca atataaacaa tatataaaac atataaaact ttacttaatt atttctacat
     2281 aaaaaccaca taaaaaagga tgtaactcta gcttttagtg taacatcctt tttttaatta
     2341 aaacttgtat ttaattttag aaagctgtct catgaccaac ataacaagct ataccattat
     2401 ccaaaaatat atcttgtaat tcttcaagat gaccttcatc aacatcaccc ttatcagaat
     2461 actcatattc cttgcctaat tgaatccatt ttgattctcc taatctgtga aatggtaata
     2521 gattaatttc taccaaatta tttttgtgca taaaagatat aatatcactt atattttcaa
     2581 cactatcatt aaacccagag ataactggta ctctaagtac taatcttcca ttccaatcag
     2641 aatttgcaag gtttgatata ttttctaata ttaaatcatt atacacacct gtttgttctt
     2701 tgtgtttttc tctatccata tgctttatat caataaatgc aaaatcaata tcgttaaata
     2761 ttttattaaa aacttcattt gatacacatg cactcgtttc aatggcagtg tgtatattta
     2821 cctcatgaca ctttaataat acttcatgta aaaattcatg ctgaagtagt ggctcacctc
     2881 cactaaatgt tactcctcca ttacttctcc aattgttaga atcacgttta attacctgca
     2941 caagttcatc tacagtgtaa ggttttgcac ataatttaaa cgcattataa taacatgagt
     3001 tgacgcattc aaaactttca cagtccttac agatgttcca atctataact ggcttattat
     3061 caagattaaa gctcaatgca ccatttttac acttaccatg acatacagta catccatttt
     3121 catattgaca agataattca ctaaacatca tatgtggtct tacagtccaa ctttctggat
     3181 ttgcacacca tttacaactc agtggacatc catttaaaaa tacagttgtt ctacaacctg
     3241 gaccatcatg aactgaaaag ctttgtacat caaaaatcat gccttctaat tgcttttgac
     3301 tactcattaa acacatgtcc ttcacaagta attgcattca atgttgaata tacccagtct
     3361 tctttctcta ggccaacaca tttgcctata ccttcatcat tagcttcaac aaaattttta
     3421 caatttttac attttggcat tacttttatc tctggacatg ctgcatcatc taaattaatc
     3481 atttgttttg ataatctaca aattcctttg gttgcatcta ctgcacaaaa attcatacaa
     3541 tcactatgct ttctcatatt ttacacccct tcatactctg ttctagcaat tacttcatct
     3601 tgtattggtt tacctaattc acaccaatat tgagtaaacc cagcaacacg taccattaat
     3661 tgacgataat tgtcaggatt tttttgtgca tcttttaatg tttctgaatc tacaacatta
     3721 tattgaatat ggaacccacc ttttcttaaa taacttcttg ttaaatctaa taatttttta
     3781 gttccttgtt gtccttttat agtagttgga tgaagtttta aattcatctg agaattttga
     3841 actactgctt ggtcccaaat agttgcagat tcaaataatg catatactcc atttttatct
     3901 gttcctgcat atgcagagac tgaaccatct gaatatgttg ttccacttag tctaccatct
     3961 ggagtagcca aggtagcagc tccctgtggc ccatgtgtag aaacagatat ttggcaaggg
     4021 tacatcttct tagcatataa agattgtgct tcttcacata cttttgataa ccatacctca
     4081 tagtttttaa gaatattatc tgcatacaaa tcattatttc catatttagg tgcatcaaga
     4141 caggccttat aaatagcatc atatttacca gtcttatcta cttttacttg gtctgccata
     4201 gaataatttc ctacttctaa tgcatcttta aatccaaaat tatttaatat ggcatccttc
     4261 atttcctcta ttgtaaatgc tttgtcatca tatacaagtt tcttaagtgc tgcaaatgaa
     4321 ttgaccatag taacagtacc acaagtttct acgtttaatg ttgcattata acgatatccc
     4381 atatttccta tatgttgacc tttgtctaaa cagtctggct ttagtagtga atttattata
     4441 gaagtatcaa attttctcca tatatctaac tcaatgttat ttgctctttc taaaacattt
     4501 attgcttgtt tataataatc tttaaatact tctatgactt cttcataagt atctaatggc
     4561 ttattatgag gctcaaatac ttgtatattc atacgatagt ctttaccatt cataagaaca
     4621 agttctaaaa tttttggatt agctataaag tgaactccag agccagcaga ttgacctgct
     4681 ccacctgcaa tagagtatgt ttttccgttt aaagttaact gtttccaaca ccctggagac
     4741 gtttccaaac atccacctaa tgcaaatgct cttgcttctt caacagtcat accttcatca
     4801 gcaaattgtt tcatcataaa tgttgttcca tttgagttgt ttacccatgc tggataacca
     4861 gaaccaagct ttgtacattc agcagctttc attaagaaat cttctggaag cttttcatca
     4921 tatagcatag ttaaactagg ttgaggagtt ctacatctca tacttgcttc taataataac
     4981 tcttctagtt cattagctcc tgtagaacca tcttctttta atccacctat agataaagta
     5041 ttaaatgtat tgcctgatag tactcctcca tttacgcccg ctgaagcaaa acagtcaata
     5101 caagtgattt ttattctata aagctctaat aattcgataa cctcttcttt tgtaattagt
     5161 cctttttcta tatcttgttc ataccaagga tataaaattt gaccaaaacg tcctatagac
     5221 ataccagata tagcatcctc atttactgat gctatatgaa ttgtataagt caactgaaga
     5281 gcttccctaa atgtacgagg ttgtttatgt gcaatatgtt ctaatatctc cactaaatct
     5341 gagtattctt ttttagcttc taaatctgtc tctatacttt ctaaatattt tgcatgtttt
     5401 gcataattta aaatccaagt ttgtaatcct tcaataactt gaataacagc ctcataaaaa
     5461 tatagacggt ccataccaat aagcccatct ccagatgcat ttcctgcaac tgccttttta
     5521 cattcttttg ccatttctat aattccatct aatccataat ttaaaggata aaaataattt
     5581 ataacttctc taccttgagg aagtgtatac cctgaatcaa acatgcatat aagtgtactc
     5641 attaggtttt cttttaaatc ataatctggc atcatttttt catagtgaaa acctaaatct
     5701 tcaacagact ttccaaccca ttcctttgcc atttttacaa gtacaggtac ttcttctttt
     5761 ctcattccga atttaccagc tattgaaacg acatttccaa aactttcagt aacatttcct
     5821 ccaccagacc caaattttgt aagttcatca gctgtgttac tggctaattt ctttgcctct
     5881 tctctcattt gttctccttg agcaacaaag aagctttcac ttacccatgg cattggaaaa
     5941 gaacctctat aatggcgtgt cttttgcatt actattttct caccaggtat tatatttggt
     6001 gtcatatgtg aaaaagcagc tttaagagct ttagctcttc taactactgg tatatcacca
     6061 tctgattttc tatattctct attataccaa tatggaaatt ccatatcagc agtagataat
     6121 gtattataat atacatctaa taagtcttct gctcttttac ttgctttttt ttcattaaat
     6181 tcacttaaat tttctttatt ttgaaaattt gatttaatat tttttgcttc caaaatactt
     6241 cttattttgt cttctttact ttgactcatt tcttcccctc cttaatcttt ctataattag
     6301 tataaaactt tttaagaatg aaaaaaaagg gtatattacc cactttttaa aataatcttt
     6361 ctatagaaaa aaataaactt gaagcaaatc taagccaaag acaatctgta tatcctaaaa
     6421 tttgttgcaa ttcattggaa tataaagatt ataaatagtt ttacttcaag ttttaatata
     6481 tactatattt atttttatta tatttaattt cacttctact tacttttttg aaacaatact
     6541 tttatattta caaattcatc taactctact gatttatctt taaatttgga actgcattta
     6601 aactcatatc atgcgttttc ccatttataa aattataata tcctgcacac cctatcatag
     6661 ctgcattatc tgtgcataaa attagaggag gatacttaac agtaatacca ttgtctttag
     6721 caagctctgt tattttagct ctaagccccg aattagaagc aaccccacca gaaagagtta
     6781 tgatattgta tcctttgtct tttactgctt ttagtgcttt agtagataaa acctctacta
     6841 cagcctcttg gaaagatgct gctacatctt caactactat ttcttcattt ttcattcttt
     6901 ttccatttag ataatttaat acagaagatt ttaatccact aaaactaaaa tcatagctat
     6961 cttcttctaa ataggctctt ggaaattcta tagcatgttt atttccattc ttggccagat
     7021 tatcaataat aggacctcct ggatagccta aattcatagc tcttgaaatc ttatcaaatg
     7081 cttctccaga tgcatcatct cttgtttttc ctaaaatttc atacttgcca taatctttta
     7141 cttcaaccaa gtgagtatga cctccagata ctattaatgt tataaaagga ggttttaaat
     7201 ccttatgctc tatatagttt gcacttagat gcccttctat atgatttact cctacaagtg
     7261 gaatattcaa agtatatgca agtgctttag cataagatag accaaccaat aaagcaccta
     7321 ctagcccagg tccatatgta actgcgatat ggtctatatc attaaaacct atatttgctt
     7381 tgtctaaagc ttcttgaaca actatatcaa tattttccac atgttttctt gaggccactt
     7441 ctggaactac accaccaaat tttttatgtg tctcgatttg agtagagatt atgtttgaaa
     7501 gtacttctct accatttttt aaaactgatg cagctgtttc gtcacaacta ctctctatag
     7561 ctaatgttat tatatcactc ataaactcta cacctctttt aattggttcc acattataat
     7621 tgcatcttct ttattatcac tgtagtattc ttttcttatt ccacccattt taaatccata
     7681 ttttctatat aaattttgtg ctattttatt tgatgctcta acttctaatg tcatagatgc
     7741 aatattatta tctttacata aatcaactat gccttttatc aacttgtccc ctattttttt
     7801 ccctctatag tcactatgaa cagccacatt atttatatgc ccttcatcaa gtataagcca
     7861 aattcccaca tatccgacaa tttttccatt taacttagct actatatacc ttgctacttc
     7921 attagacaac tcttttctaa aagactcttt tgaccaataa tcttcaaaac aatttttttc
     7981 tacttcaaaa acttcatcta tatcttctgt agtcatttct tctattttta tatcatctac
     8041 tattagtttt gtattatctt ccattattta gcctcttcat tttttcatca tattgtactt
     8101 cagcttgaga ttttctgata tacataggat ttatcgtata acaagtatgt acatctatat
     8161 ttttattata tttatttaat gctaaagagc ataagcttcc tgcgttagat acattattag
     8221 ctggtgaagg tatttttata ttttttatat tctctaactt gtctttatat ttgtatacag
     8281 cttctccaac tattataaat tcttcattag ttgatacaat ttcatctact aaagcatcaa
     8341 actctacaac atcaacacca tctaattcta tcatctctcc attttcaaac ttgtatttag
     8401 ctgaataaac ttgatttttc tgtgcatcta atatacaaca tatctttcta tcacagaaat
     8461 ttatgttatt tgctagagat tccaaagaat ttactgctat tattggtata ttatttacat
     8521 gggccatagc ttttactgta gccataccta ttctaagtcc tgtaaacgaa cctggaccta
     8581 tacatacagc taataaatcc atatctttta tacttaaatc actcatactc aacatatttt
     8641 ctatcattgg cataagcttt tgtgaatgag tttttttgtt gtttactgta aactcacata
     8701 ttaagttatc atcttcaaca actgctacac ttgctgccat agaagacgta tccataccta
     8761 gtattttcat tatttaatca actcctcaac tatctcttta taaaactctc cttttggagt
     8821 taatatcatt tctcttcctt catctttata tctcaattct atattcaaat attcttttgg
     8881 aagtatatct tctattagat tagcccattc tattatacag attccatttg aatttacata
     8941 ttcatcataa ccaatatcat acatttcatc actactacct attctataaa catcaaaatg
     9001 atacaaaggt atttttcctt catattcatt tattatagta aaagtaggac ttgttatata
     9061 atcttctatc cctaatgaat ctgccaaact ttgtgtcatt gtagttttac cagctcctaa
     9121 atctcctact aaacagataa cactgccttc ttttaaaagt ttacctaatt tatatcctat
     9181 ttctctagtt ttattttcat tttctaaata tatttttgcc atcaattcat ctccattttg
     9241 atataatcgt catgtgaatt tatatcactg cttaattaat tatagtataa gcctagatta
     9301 ttataaagta cttgtcttta tcttttttta tctttcacta ttgatggctg tatctacacc
     9361 ttatatacaa tcatcaatag caatttttac atactctttt gctttatggt tttgcttcaa
     9421 tgcttcttta catattctct cagattgctc ttttacatac ttaagagact tatagttctc
     9481 ttttactgct tcaatacaca ttttttctgt ttgatttttt atatacataa gcatttttcc
     9541 attttgtctt acaatctcta tacatatatc ttctgtatac ttatctatgt aacttagtac
     9601 atctttaata ttatattttt tatttaaata tcttatagca tttatacata tttcttctgt
     9661 cttattgttg acaaattgta tacttcttcc atcttgtttg acagcttcta tacatatttt
     9721 atcagtttgt ttatttacat attgtaatgc cataccattt tgtcttacgg attctataca
     9781 cattttttcc gtttgatttt ttacatattt taatgcttta taattttgct taatggcctc
     9841 tatacataat tcttcactct gttctctaac gtatgcaaga cacctgccat cttttttaat
     9901 tgcttctaaa catatccttc ttgtttgagt ctctaaagcc tttagtatac ttacaacatc
     9961 tatattggaa gaatgactta ttgcttttat aataagccct tctgttttat ttcgtacata
    10021 cttaagtgca ttacaatctt tttttatagc ttcaagacac aagtattctg tttgttcttt
    10081 tacatattca agtgctttat aattatactt tattgcttct aaacataatt cttcggtttg
    10141 attttttaca tactctaatg ccttgtaatt ctgccttatt gctaaaatac acataccatc
    10201 agtttgattt tctatatttt ttaattcacg ataatcgtat tttacccttt ccatattttc
    10261 aatatattca ttagtgatat actttaaaga atctcctaat tttgtatcat aaataccaat
    10321 atgaatatct ttccaattac tattaaaata atacccttta tcatctaaaa ataatttttt
    10381 tattttttta gataaatgca tgaatataca cccctatcat tattaaatta gttattatta
    10441 aaagtttgat tattaatctt tatagttttt taataataac tttatttttt gtttattata
    10501 aattcgctct gtatattgta gatacctttt tcatatttta atagttaatc gttttaattc
    10561 ttcctttatt ttaaaattta aatttgtcta aatatttctt attttatata cattttttga
    10621 taaattaata aagccataat atatttaata tatcaatcac aatataggac tgaagttata
    10681 aataaattat ggctattcaa tacatttaat tttatatttt cttgtctaat tatttcttta
    10741 ttatatttcc cattttttta taaagtggca atgttactaa agatattata acagccttca
    10801 gcacattaaa tggagctatc atccaaatta caaaagtgaa taaatcatga acttgaggat
    10861 ttatagctga acctaaacct attatagcat ctagtcccat aagttgacca taaagtggca
    10921 acattacaaa ataatttact aatgaaccaa ttatagtcat agatattgtt cctaatacaa
    10981 aaccaatcag tacacctttt aaatctccac ttctcttata tgaataacaa agtatcatta
    11041 cataagaacc accaattaat atatttgcaa attctcctat tcctccagtt gttgttgctg
    11101 ttattgcttg taacacattt tttactacaa ctattccaaa tccaactaat ggtcctagag
    11161 acattcctcc gaatattgct ggaatatctg ataagtcaat ttttaagaaa tctggaaata
    11221 tacctggtat tggtgcttga ataaacatta ataaataaga tattgcagat aaaattgaca
    11281 ttactactaa agttcttgtt gaaatcatat tgcctctttt tgctgtgttt tgcataatta
    11341 ttttccccct aatcaatatg tttatatttt ttaggaaaaa caaaaaactc gaagacccta
    11401 tcttcgagtt tttatggata tagtttaaac aaactaaaat aaacttattt atcttccata
    11461 aatatcttct ctcatccaga ctttactgtc ggctttggaa tctcaccaaa tcaaccacct
    11521 aagtagttcg cgggctatac cgccggtagg gaatttcacc ctgccccgaa gatcttcttt
    11581 gattttttaa ttttccttac actcttatat tagttaattt tttaataatt gtcaagttat
    11641 tttttctcat acactacttt gccatttatt atagtgtaaa gtacattact ttgaatctct
    11701 agtggagaat tatcccaaat tactaggtct gcatctttac caacttctat agagcctact
    11761 ctatcttcta ttcctaaagc cttagctgga tttatcgtaa ttgattctat tgctttttct
    11821 tctttcatac catgttttac tgcaatacct gcacatattg gaagatattg aactggtatt
    11881 actggatggt ctgtcattat acatacatca agacctgcat ttgaaagtat tcctgctgta
    11941 ttaaatgtca aatttctaag ctcaaattta gacctttctg acaaagatgg ccctactatt
    12001 acaggaaacc cttcttccac taattcatct acaataagat gaccttctgt acaatggtct
    12061 aatgtaagct ttaaattaaa ttcttttgca attcttattg ctgtaaacat atcatctgct
    12121 ctatgagcat gtgccttaaa aggaatttct cttcttaaaa ctggtattaa actttccatt
    12181 tttatatcat attcaggttt ttcacagtct tcatcatcat gactctcata catatcaata
    12241 tcctctaaat attcttctgc tttttttagg ttttctctaa gtaaagcagc tatagccatt
    12301 ctagtttgag gagatttatc atcctgacca taacagctct ttggattttc tccaaaagca
    12361 attttagatg caactggatt ttttattacc attttatcaa ttctttttcc acatgtcttt
    12421 atagctatac attgaccacc cataacattt gcacttccag gggttgtaca aacagaagtt
    12481 attccacctt caaaagcttc tttaaaagtt ctatccattg gattaattcc atctatagga
    12541 tttaattgtg gtgttattgg gtcagtctct tcatttccat ctgctccttc aaaccctata
    12601 ccatcttccc ataaacctaa gtgagtatga gcatctataa atccaggaaa cacaagttta
    12661 ttactagcat ctattacctc tacatctaaa ggagctatca aatcttctcc aatttctatt
    12721 atttttcctt catctataag tatatctcca tgaattatcc catttgttat ggtatttatt
    12781 ttaccatttt ttataaaaat cattcatttt cctcctgatt ttcaagacta tcgttcttat
    12841 tttaatatat tttcttacta tatactttta caaattttta acatttatac actcgagtaa
    12901 tcttatttac aaatatagaa tctaagtact ataaccatac ataaacttat ctattttttc
    12961 attgataagg caactctttt tttgtttaag tcaattccaa tgacttttac atctactata
    13021 tcacctactg taacaatact catagggtct ttcacaaaac tattactcat ttctgattta
    13081 tgaactaatc cgtcattttt tattccaata tctacaaatg caccaaagtc tacaacattt
    13141 cttattgtac cttttaaagt cataccctct tgtatatctt caatttttaa gacatcagtt
    13201 ctaagtatag gttttatacc ttcttctctt gggtctcttc ctggtttttt gatttcagct
    13261 atgatatctt ttaacgtaac ttgtcctact tccagttttt cacttaattc cctcaagcca
    13321 atttctttta ttttttcatc tatttcacct atattcttat tctttacatc atctaaagaa
    13381 tatccaatta tctcaatcat ttttttacat atatcataac tttcaggatg cacacctgta
    13441 ttatctagtg gatttttacc ctctaagatt ctcataaatc cagcacattg agtaaatgct
    13501 tgaggtccta gtctttttac ttttttcaat tgtgctcttg acgtaaaatc tccattttct
    13561 tctctatatg ctattatatt ttttgctata gctttactta ttcctgcaac atgttctagt
    13621 agggagtatg atgcagtatt taaatctact ccaacactat ttacagaatc ttcaacaact
    13681 ccatctagta cttcttctaa tctctttttg tttaaatcat gctgatattg accaacacca
    13741 atacttttag ggtctatttt tacaagctct gctaatgggt cttgtaatct tctagctata
    13801 gatatagctc ctcttattga tacatttata tctggatgtt cctcatttgc aagttcagat
    13861 gctgaataaa cagatgctcc tgcttcacta actatcacat actgcacttc gctatctatt
    13921 tcttttatca tttctgatac aaaagtttct gattctctag atgcagtacc atttccaatt
    13981 gaaattatat ctatatcgta tttttcaatc aatcctttta aaactttctt tgctccctct
    14041 acatcatttt gagggtctgt tggatatact gttgtataat ctagtagttt accattttta
    14101 tctactacag ctattttaca tccagttcta aatgcagggt caaatcccat aactactttg
    14161 tctttaactg gtggttgaag caataaactt ttaacattct ttccaaatac acttatagct
    14221 ctttcttgag ctatttctgt taaatgattt cttatttctc tttctattga tgggaaaatt
    14281 aatcttttat aagaatcttc tattgaactt acgatttctt ctttattttt gaaattttta
    14341 tcatttacat attcgtttat tatgtaattt aaaactttat cattatttat ttccaactta
    14401 acctttagga agctttcttt ttctcctctg tttattgcca aaactctatg tggagccata
    14461 ctttttactg cttcgctata atcataatac atatcataaa ccgatttctc atcagtagca
    14521 cttttgctaa ctatcatacc ttctcttaaa gctaattctc ttatatactt tctaatctta
    14581 gcatcatctg agactagttc tgcaattata tctctagcac cttttagagc atcttcaata
    14641 gaaagaactt ctttctcttc atcaagataa ttctttgcct ctatttctat attatctaaa
    14701 ttattctcta gtattgacaa tgctaaattt tctaatcctt tttcttttgc tattgttgct
    14761 ctagttcttt tcttttgttt atatggagca tatatatctt ctacttcttg taatgttttt
    14821 gctttttcta tattttgtgt tatttcatct gttaatttcc cttgttcatc tattagtcta
    14881 actacatcat cttttctact ttgaagattt ctcaaataca ttaatttttc ataaaattct
    14941 cttagtgtaa catcactcat ttcgccagtc atttcttttc tatatctagc tataaatggt
    15001 attgtatttc cttcatcaat aagttttaat gtattattta tttgttcatc tctaagatta
    15061 aactcttttt ttaagatttg atttatatcc aatttattca ctcctaatgt tagtttttca
    15121 taagtataac aaaacagaac gaaggctgag ggttatcgtc ctcagcctaa gtgctgtaag
    15181 tgcatattaa gcactaacct tcttatacat atatcttaat tacagtgcat atttattaaa
    15241 ctattttatt catttttaag tattcattaa taaacaagtc tatatttcca ttcattacac
    15301 tatctacatt tccaaactca gcatttgttc tatggtcttt aaccaattta tatggttgaa
    15361 aaacatatga cctaatttga ctaccccaag ttatttgtga gtatttgcct tgtatatctt
    15421 ctattttttc cttttgctct aactctttta attctatcaa tttcgccatt aaaagtctca
    15481 tagctctgtc cttatttaga tgctgagacc tttcattttg gcactgaact actacaccag
    15541 tagggatatg tgttattcta actgctgaat cagtcgtatt tacatgttgc cctccagctc
    15601 cagaagctct atatgtgtct atctttaaat ctgatggatt aatttctact tctatatttt
    15661 catctaactc tggtgttaca tctatagatg caaatgaagt atgtcttttt cctgatgggt
    15721 caaatggaga tattctcact aatctatgaa ctcctttttc actctttaaa tacccataag
    15781 catttgttcc ttctacaaga attgtagctg tctttatccc tgcttcaggg tctgatatta
    15841 tatctaaaag cttaacttta taacccttac tttctgacca tcttatatac attctcaaaa
    15901 gcatttgagc ccaatcttga gcatctaagc caccagtacc agcatttata gataatatag
    15961 cattattttt atcatactct ccatttagaa gagtctttat tttcatttca gataattttt
    16021 cttccattga ttctatggac ttttctatat ctctttctac agaatcatca tcttcttcta
    16081 aacctatttc aattaatact tctatatctt ctaataagct ttttaagctt tcatattctt
    16141 ctaatgtctc ttttaaagat ttattttctt gaagtactct ttgagcgact tcattatcat
    16201 tccaaaaatc ttgttgattc atcttttctt cattaatttg tatatttttc atcaatgaag
    16261 ctatgtcaaa gagaaaccct caattcttct aaatttgaag ccatctgctc catactatgt
    16321 ctatactctt gtaactttaa catttaatcc tccgagatta tcttccacaa cagtttttat
    16381 attttttacc acttccacaa gggcataagt catttcttcc aaccttttca tcttttttaa
    16441 cagttttact tgttggaagt gttccatctg atggagaaga tagattttca acatctacaa
    16501 ctgcttttct ttccactgga gtttctattg ttatattaaa taaatatctt acagtatctt
    16561 ctttgatatg tttattcatt tcatcaaaca tatcaaaacc ttccatttta taagcaataa
    16621 ctgggtcttg ctgacctaca gcacgaagac caataccttg gcgtaattgg tccatagcat
    16681 ctatatggtc tatccaatgg ttatcaacag cttgaagtaa gattactctc tcaacttctc
    16741 tcattctctc atagccaact tgttcttctt ttgaagtata tatcttcata gctatttcat
    16801 aaactttttc tgttatttca actggattaa gtttttctat ttctggtatt tctatgctac
    16861 cttttggcaa gaacagatta tacatatgtt ctttgaatcc ttcttcatca aatcctttat
    16921 cttgtgtata aagagtaact gcttcttcta ttatagaatg agtcattgat tgtatttgtt
    16981 cttgtaaatc ttcaccttct aatacacgtt ttctctctgc atatattatt tctctttgtt
    17041 tattcataac atcatcatat tgaagtacat gctttcttat tccaaagttt ttaccttcga
    17101 ctttcttttg tgctccttca atagacttag ataatattct atgttctatc ggcatgtctt
    17161 cttcaagacc tattttatct acaataccag atattctatc actaccaaac aatctcataa
    17221 ggtcatcatc aagacctata tagaatcttg aactccctgg gtcaccttga cgtccagcac
    17281 gacctctcaa ctggttatct attcttcgag attcgtgtct ttctgtacct attatagcaa
    17341 gtccacctgc ttctacaact tgtttttgtt cttcttgtgt ttgttgctta aacttttcaa
    17401 atagtttttc atactcttct ctagcttcaa atagaatttc attacctttt acaggtatac
    17461 cttctattgg agtatctact ctatttacta tttcttcttt aaatccattt cttctcattt
    17521 ctctcttagt taaaaagtct ggatttccac ctagaactat atctgtacca cgaccagcca
    17581 tattagtagc tattgtaaca gctccaagtc taccagcttg tgctattatt tctgcctctt
    17641 tatcatgatg cttagcattt aatacctcgt gctttatgcc ctttttcttt agtatttgtg
    17701 ataataattc tgatttttct attgatactg tacctacaag tattggttga ttgactttgt
    17761 gcctttcaat tatttcttgt gctacagcat taaatttacc tatttcactt ttgtatacac
    17821 agtctggtaa gtcttccctt atcataagct tattagtagg tacttggaaa acatccattt
    17881 tgtagattgc tttaaactct tcttcttctg tttttgctgt accagtcatc ccagaaagtt
    17941 ttttgtacat tctaaagtaa ttttggaatg ttacagtagc tagtgtttta gattctcttt
    18001 gtattttaag accttcttta gcctctattg cttgatgtaa accttctgaa tatcttctac
    18061 caaacataag tctacctgtg aactcatcta ctattactat ttctccatct ttagcaacat
    18121 aatcaacatc tttcttcatt attacatgtg ctcttaaagc ttgatttata tgatggtata
    18181 attctgtatg agatatatct gttatattat caacattaaa atacacttct gctttttgta
    18241 tacccgatgc tgttagagat acagccttat ctttttcttc taattcataa tcatctggtt
    18301 ttaaggttaa aacaaatgtg tttgcatctg aatataagtg agttgatttg tctccaggtc
    18361 cagatatgat aagtggtgtt ctagcctcat ctattagtat agagtcaacc tcatccacta
    18421 tagcataatt taatcctctt tgaactcttt gctctttgtg tattaccata ttgtccttta
    18481 gatagtcaaa tccatattca ttatttgtac cataagttat atcacaatca tattgttctt
    18541 ttctaacttt tggattttga ccgtgtataa taacgcctac actcattcct aaaaactcat
    18601 agatttttgc catttggtct ctatcacgtt ttgcaaggta atcatttact gttacaacat
    18661 gtacaccttt tccagtaagc gcattcaaat aaacaggagc agttgcaacc aaagttttac
    18721 cttcacctgt tttcatttct gctattcttc cttgatgtag tactatacct ccaatcattt
    18781 gaactctgta gtgtctaagt cctaacactc tcttagaaac ttctctaact actgcaaaag
    18841 cttctggaag tatatcatct atactttcac catttgctaa tctttcttta aatatatttg
    18901 tcatattttt aagttccgag tctgccatag attcaaattt aggttctaat gaatctatta
    18961 tatcaacagt tttattgaac ttttttaatt cttttttatc tgccatgttg aataaattat
    19021 ccataaagct catattttac atctctcctc gttctttatt gtactattat tcattttacc
    19081 ataaaaacta tacttttaat attaatttaa aaagctatcg ataatttatt tatcgatagc
    19141 taatatgatt aaatctaaat tgctttattc atgttctatt ataccatatc caccattatt
    19201 acgcttataa actactgcta tttcatctga atctatgttc ttgaacatat aaaaatcatg
    19261 tcctattaat tccatctgta atacagcttc ttcttcactc attggcttaa cactaaattt
    19321 cttacgtctt tgaattacta attcttgatt ttcatcccca taattctctt gttcatcagc
    19381 tagatttatc tctgcatctt ggaatcttat actcttgttg tctttatgtt tatcttgtaa
    19441 tctcttttta tattttttta attgtttact taatttatca tatacaacat ctatagctgt
    19501 gtataaattt tcctgcatat cttctgctct aattatagga ccatttacag tagctattgt
    19561 tacttctatt ttttgtcttg cttttctcgc acttactgta acttttacat cagtgtctgg
    19621 atgaatgtaa ttatctaact tacttagttt agtttcgatt ttatttttta ttgcatcagt
    19681 taattcgatt tgttttccag atataattat gttcataaat acatctccct tcaattatgc
    19741 cagtataaga aaatttatac tttaataata tattctacac gaactctcat aatcctcttt
    19801 ttattattta cttttatgat tttttctatt caattgatag aattatttat catttttagt
    19861 tacattgata cttatgtgga ttaatattat tacaaacacc tgtggataat gtggataaaa
    19921 ctgttaataa ctcttgtttt tgtttattta aagctatttt tactcctatt tctatccaca
    19981 aaaatctcca ttaattgttt attaatatgt gaattcatgt cataagatgg taattttagg
    20041 aaattatgtg tattatatgc caatatacct ttataatttt ttaataaata taaaccctct
    20101 aaaacctcca taatctatct tttctttcat tatttatatt taaaactaat taaaagt
//
LOCUS       DAYAPA010000070        19799 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.070, whole genome shotgun sequence.
ACCESSION   DAYAPA010000070 DAYAPA010000000
VERSION     DAYAPA010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19799
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.070"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(446..1447)
                     /gene="trpS"
                     /locus_tag="ACGI59_002326"
     CDS             complement(446..1447)
                     /gene="trpS"
                     /locus_tag="ACGI59_002326"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFG9740111.1"
                     /translation="MSEKKVIFSGAQPSGKMTLGNYLGAIKNWTELQDNYDCYYSIVD
                     LHAITVPQDPKVLRANTIELLAQYIACGLDPEKNTIFIQSHVKEHVELMWVLNTMTYM
                     GELSRMTQFKDKSQKSEANLNAGLFTYPVLMAADILLYQTDLVPVGDDQKQHLELARD
                     LANRFNNRFSPTFVVPEGYYPKGGARVMSLQEPTKKMSKSDSNENAFILLADDSDSIK
                     RKIKRSVTDSLGVVKYNDEQPGIKNLIDIYSNLSKKSVEEIVNMYEGKGYGIFKEDVA
                     EVVSEALRPIREKYVDLLNNKDYLEKIYSMGAEKAEKQARKTLRKVYKKVGFIERRY"
     gene            complement(1470..2168)
                     /locus_tag="ACGI59_002327"
     CDS             complement(1470..2168)
                     /locus_tag="ACGI59_002327"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFG9740112.1"
                     /translation="MNVIGIIGAMDEEVSILVDLMDIRETIKKASLEFYKGILEGKNV
                     VLVKCGIGKVNSALCAQILISEFKVDAIVNTGVAGALNEKLDVNDIVISTDAIQYDVD
                     TTAFGDPKGVIPRMKTSVFKADERLIDAAYKSSVEEVKTHKVLKGRVVTGDKFINSKE
                     LKEELVNDFGGYCGEMEGGAIAHVCYLNNTPFVIIRAMSDKADGSADVTYDVFVHDAA
                     NNSKDIVLNMLKSI"
     misc_binding    complement(2211..2451)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(2542..3945)
                     /locus_tag="ACGI59_002328"
     CDS             complement(2542..3945)
                     /locus_tag="ACGI59_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFG9740113.1"
                     /translation="MEKKKIGLWSLVFMNVSALYGIRWIAKSTASSFGLGLGAIPMWL
                     LFSFIYFVPGALICAELASTYQKGDGGLYDWVKEAFGEKYGFLVSWLNWTAKIFWYSS
                     FLTFLAVNVSYTIGNPALADNKMFVLILSLIIFWALSLIATKGISFTKIFTNTGALGS
                     TIPSAILIIFSFVAVFVLKKHDIASTYTIASMTPKLNMDAFVAISAIMFGLAGAETAA
                     NFITEIDKPEKNFPKAILISAGIVASLYVLGSIAITMIIPPDQITASKGVLDALSAVC
                     ASLGIGSWLVQLIAFGIAFSVLGAIVLYIASPIKMLFGSVKKGIFPDSLVEVNEHKIP
                     SKAVILQAIIVTIILLVTTLMPSVDAIYNVLVTMTALTALFPYVLLYASYIKLRKERP
                     DEIRPYTMAKSTSTCLGLAKMVLVVTVVGILLSAAPVMPTFAENVIYEIEMIGGGLLV
                     ILSGLWLWNRYEKKTAK"
     gene            4442..5518
                     /locus_tag="ACGI59_002329"
     CDS             4442..5518
                     /locus_tag="ACGI59_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HFG9740114.1"
                     /translation="MKAQRLNAVLEQMKKDDISQMLVSDPTSIFYLTGVLIHPGERLL
                     ALYLNLNGNNKLFINELFPVSEDLGVEMVWFNDTQNPVEIITEHIDKNATMGVDKNWP
                     ARFLLNLIELGGGSKFVNSSYIIDTLRMCKDEEEKELMRIASKLNDKAMEQLKATVSG
                     ELTEKQLVGKLSKIYEDLGTDGFSFDPIIGFGPNGANPHGEPGNALVKPGDAIILDIG
                     CIKDNYCADMTRTVFYKEIPEKGREIFEIVLEANKRAEAIVKPGVRFCDIDAAARDYI
                     TEKGYGQYFTHRTGHSIGLEVHDKGDVSSINTDTVQPGMIFSIEPGIYLPGEFGVRIE
                     DLVLVTEDGCEILNKHDKEICVVG"
     gene            complement(5621..6463)
                     /locus_tag="ACGI59_002330"
     CDS             complement(5621..6463)
                     /locus_tag="ACGI59_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFG9740115.1"
                     /translation="MDKIKLIVDSACDLPDDIIEKYNIEVVGLNVSFGEESYISGKEI
                     DNETFYRKMSESKVLPKTSCPSPDKFLEAYHCQEESVLVINISSGLSGTYNSALLAKD
                     MFEKEGNNKKVEVIDSLSGSIGIGQLVLKAAILIKEGKEIEEIVSIINKYKDNPPFFG
                     TLETLENAIKGGRINPIAGKIINTLNFKAIIQVADGVVTPIDKARGEGNSLKKLITLV
                     ESKIKDKEEKVLFIGHANCPEKAQKVREVMEKDVKYKDVVICEIGSVMGTYTSKGAIL
                     ITAI"
     gene            6647..7219
                     /locus_tag="ACGI59_002331"
     CDS             6647..7219
                     /locus_tag="ACGI59_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFG9740116.1"
                     /translation="MKTNNMKQAILSSATNLIAKNGVQNTSLADIAKDVNISKGTLYY
                     HYASKDDIIYDIADTHLEVITSAILNCVKNVKSKNSQIEMVNLILEKISTIESRGRVH
                     MYLICEAITSNNDLKERIRLKYIEWRTTLKSEIVESLEKYNSSENEQDAESFSFLLMS
                     IVDGLVVQSLLKTEKIPYENIASFLVNHWI"
     gene            complement(7382..7654)
                     /locus_tag="ACGI59_002332"
     CDS             complement(7382..7654)
                     /locus_tag="ACGI59_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9740117.1"
                     /translation="MLRERFVVRNHHNPHTSGCCGTGGEGCCGGDNHEHSHQHSHSHG
                     HEHEHVNGVGHDHNHDDGFSGTHEHSHEHTHTHGHEHSDGHTHEHK"
     gene            complement(7781..8653)
                     /locus_tag="ACGI59_002333"
     CDS             complement(7781..8653)
                     /locus_tag="ACGI59_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFG9740118.1"
                     /translation="MYGESKKLSIDNNIFLQYCLIILGMILSTLGINLYLAPAKLLSG
                     GVAGICVILYKLFGINQGISSFLMNIPIFIIARKYFDNKFLLISFVNMLLFSLALGLT
                     QDIAKYFPIDDTMLQCIYGGALTGIGMGLTFKARATAGGLDIIAAIMKRKYDIPMKNT
                     FLFINFFVVCAGAFLFGAKLVMYTLITMYIISFTMDIGKDCFDRKKSILLISNKYDEI
                     SKVIMNKMGRGVTFLEAEGAYTQNKKKMIYCIISANEIGKFKDLIYDMDSEAFISVNN
                     VEEVKGGGFKDKFL"
     gene            complement(8949..12056)
                     /gene="ileS"
                     /locus_tag="ACGI59_002334"
     CDS             complement(8949..12056)
                     /gene="ileS"
                     /locus_tag="ACGI59_002334"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFG9740119.1"
                     /translation="MAKFKPLVDSSVKQAEAQVFDYWKDINILEKTLEKGKDDPSFVF
                     YEGPPTANGNPGVHHVLSRTLKDSVCRYKTMSGYQVKRKAGWDTHGLPVEIQVEKELG
                     LTSKQQIEEYGIAEFNQKCRESVFSFEKQWRIMTERMAYEVDLDNPYITLDNNYIESV
                     WWILNKFNKEGYIYEGHKILPYCPRCGTGLASHEVAQGYKEVKTNTVIAKFKKKDADE
                     YFLAWTTTPWTLPSNVSLTVNADVDYLKVKKGDEVYYVSKPLADKVLGEDYEVLEEMK
                     GKDLEGLEYEQLMPFVEVDKKAFFITVGDYVTTEDGTGIVHTAPAFGEDDYNTGKRYD
                     LPVIQPVDETGKFTSTPWEGRFVMEDGLDVEIIKWLASENKLYSKEKVVHNYPHCWRC
                     QTPLVYYAKPSWYIEMTKLKDKLIENNNGVKWFPSFVGEKRFGNWLENLNDWAISRTR
                     YWGTPLPIWRCECGHTDSVGSRAELAEKAIEDVNPETVELHRPYVDDIHLKCEKCGKP
                     MTRVTEVIDCWFDSGAMPFAQHHYPFENKENFDQLFPADYISEGIDQTRGWFYSLLAV
                     STFVLGKAPYKSVLVPDLVLDKDGKKMSKSRGNTVNPMELFDQYGADALRWYLLYVSP
                     PWTPTKFDMDGLKEIQSKFIGTMKNVYNFFTLYANTDDINPTEFFVEYKDRPELDRWI
                     LSKFNNLMKDVEENLAIFELNKTLRMIQDFINDDLSNWYIRRSRRRFWATELTEDKKA
                     VYNTTYELLHGLCRAIAPFAPYMSEEMYRNLTGEVSVHLAEYPKCNEELVDTKLEEKM
                     DLAKNLVTLGRASREVERIKVRQPLQKVLVDGKFEDTISDVVDLIKEELNVKEVIFAK
                     DLDEYMNFSLKPNFKEAGPLLGSKMNLFVGALSKLNAHETANKLEKGETLTVDLDGEA
                     FEFNKDLVLIGITAKEGFNVSVENNLFVILDTKLTEELINEGYAREFISKVQQLRKSN
                     NFEVLDNIVIEYCGDDEIAKAVDHFNEYIKSETLALEINRVDDKSLEEQNLNDHMTGI
                     KVIKK"
     misc_binding    complement(12149..12381)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(12556..13083)
                     /locus_tag="ACGI59_002335"
     CDS             complement(12556..13083)
                     /locus_tag="ACGI59_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HFG9740120.1"
                     /translation="MLTPIEIENKEFKKGLRGYRDEEVDEFLDIIKEDYESLCRENTA
                     LKEKLGLYQDQVNKYENIEETLKATLITAQSAAEDTCSAANKKAKIIVEEADLKARQI
                     IEQANNRVIEIRKEYDALVKEFKIFRNKFKSLLEDEIRSVDEIFYDVDEKCVNAFEGT
                     TVYNYSNDEAATTLE"
     gene            complement(13118..13903)
                     /locus_tag="ACGI59_002336"
     CDS             complement(13118..13903)
                     /locus_tag="ACGI59_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlmH/Sll1252 family protein"
                     /protein_id="HFG9740121.1"
                     /translation="MDKLKLTNHIKDIDLKNKMFKVIDKANSCIKNYDVKSTEFLNPY
                     EVKNAVAILNSTNEIKYSVDGGYEQAERSTVFIYPFYMEYEDIEDTLRFLQIEGNFKF
                     KNISHKDYLGSILGLGIKREKIGDIIIHDSFCQVVVSSDICDFIIVNLEKVSRNNVIV
                     KEISRENIVNSSSKYKEVSFTVSSDRLDCVISGIYNISRQDSAKYINGEKVHVNYEKI
                     TSTSKIVKNDDLISIRGKGRAKVTQIGDITKKGKIKVQARLIV"
     gene            complement(13913..14170)
                     /locus_tag="ACGI59_002337"
     CDS             complement(13913..14170)
                     /locus_tag="ACGI59_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HFG9740122.1"
                     /translation="MGTIRIALYYLLDIIAWMIVIKSLMTWFPNGRQSKIFDILENLT
                     EPIEGPIRSIMYKYTNGPIDFSPLIAIVLLMFLRQLVLVIF"
     gene            complement(14189..14647)
                     /locus_tag="ACGI59_002338"
     CDS             complement(14189..14647)
                     /locus_tag="ACGI59_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HFG9740123.1"
                     /translation="MSNGIISKFKNWIVDEEEDYIEDEYESGMDDIVQEEEMNSGFST
                     AKANKIVNLHTTSQMKVVIVEPKVYDEAATIADHLKQRRAVIVNLEGLTNSEVRKSIF
                     NFMNGAVYVLDGSIQKVSKSIFILAPNNVDIDANMKKELESKAFFPWQNK"
     gene            complement(14687..15391)
                     /locus_tag="ACGI59_002339"
     CDS             complement(14687..15391)
                     /locus_tag="ACGI59_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HFG9740124.1"
                     /translation="MGSIKDNLSEIRNKIDKAAKKVDRDSKEITLLAVTKTVDVDLVN
                     EAMREGITSVGENKPQELARKYDIIGDKLNWHLIGTLQTNKVKYIIDKVCMIHSLDRI
                     ALCEEIQKRAEQINREIDCLVQVNISKEESKHGLDREEVVDFVKEVSEKYKNIKIKGL
                     MTMAPFIEDEDEIRGVFKGLKDLSKQIKELNIPNVGMDVLSMGMSHDYEIAIEEGATI
                     VRVGTSIFGERNYNKK"
     gene            complement(15401..16036)
                     /locus_tag="ACGI59_002340"
     CDS             complement(15401..16036)
                     /locus_tag="ACGI59_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HFG9740125.1"
                     /translation="MKKVKYTRLVILGILIYLVFNILVMIISKNVDTLVLKNEKVDAK
                     IHVKGTIEKDANDQSNYLSTYISKEDSNNFEKNQNVIIEYHEQKLDAKVYKIYENNNK
                     IMVKLKISNQIVANQDTSVEEFDIIYNQMECLKIPKTSIKTKDNKRGVYVIDEQSQSV
                     KFVILEGITYENESSVFVDYYKNDINGVTSVNLYDKIILRPNSINTNIRIK"
     gene            16417..17457
                     /locus_tag="ACGI59_002341"
     CDS             16417..17457
                     /locus_tag="ACGI59_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFG9740126.1"
                     /translation="MSEILLGTGLLLLVLCCFTIFSYKAPYGMKAMGALANAACATFL
                     VEAFNLSLLGDVLGMEFFRSVGAANGSLGGVAAAILVPLALGVSPVYAVLIGLSCLDF
                     SILSGFVAGYFVSFLIKFIEKKVPPGVDLVVVILLCAPLSRLIASNVDPLVNSTLLQI
                     GQVLIEATNISPIIMGIILGGLITVVGTSPLSSMALTSIIGLTGLPMAIGALGAFGSA
                     FMNSTLFAKLKLGSKKDIISVAIEPLTQSDIISANPIPIYSTNFFGGAFSGIIVALMG
                     LVNMTPGTATPVAGFAIMFAYNPVQQVLITAGLCALFNVIAGFLGSKIFSTFKVLTAG
                     EIRGTDVEEEIA"
     gene            complement(17920..19278)
                     /locus_tag="ACGI59_002342"
     CDS             complement(17920..19278)
                     /locus_tag="ACGI59_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008624114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HFG9740127.1"
                     /translation="MVNVGNILETIKMIDEEQLDIRTITMGISLLDCIDPDGDKAREK
                     IYNKIMSSAKDLVKVGKDIEREFGIPIVNKRVSVTPISIIAGATDENDYVKFAQTLDK
                     AAEDLGIDFIGGFSALVQKGYTKGDKILIKSIPKALASTNKVCASVNVGSTRCGINMD
                     AVKEMGEIIKETAELTKDAKGFGCAKLVVFCNAVEDNPFMAGAFHGVGEADRIINVGV
                     SGPGVVKRALEKVKGEPFDVVSETVKKTAFKITRVGQLVAKEASSRLDVPFGIIDLSL
                     APTPAIGDSVANILEEMGLEVVGTHGTTAALALLNDAVKKGGVMACSHVGGLSGAFIP
                     VSEDAGMIDAVIKGALSIDKLEAMTAICSVGLDMIAVPGNTTAGTLGAMIADEAAIGM
                     INNKTTAVRIIPAPGCDVGDMVEFGGLLGRAPVMPINTNSSELFTQRGGRIPAPIHSF
                     KN"
     gene            complement(19301..19570)
                     /locus_tag="ACGI59_002343"
     CDS             complement(19301..19570)
                     /locus_tag="ACGI59_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HFG9740128.1"
                     /translation="MKAILTVIGKDKPGIVAGISDELYKQNINIVDISQKILQDYFTM
                     IMIVDLEKSLNSFEKTVEDLVERGKKLSVDVKIQREEIFNSMHNL"
BASE COUNT         6376 a         3442 c         2261 g         7720 t
ORIGIN      
        1 aaaatatttt tttgatatat tattgacaat tttcattata agagttaaaa ttaactcata
       61 aattacatat tgggtataaa atacaatgta tgttagaagt tgtttcagaa ggctatggtt
      121 ttctgatttt tttatgctca gtagtgatat gcaaaaaaga caaagttgac atcagttaac
      181 ttttaccttt attctacttt ccccaaagta agaataatct aacccctttg agatgagcaa
      241 aagctcatct ttttttatta tcaaaagatg ctgtctcaaa atagagataa tcctcttttt
      301 cattgttgaa atgattaaaa tatattttat ttcatacaaa aaagagtgcc tcatgaacaa
      361 aagttcactt tgagactctc tctttcatat catatacctt tttaataact ttaaaattta
      421 tttatcaagt aaagatagct aataattagt atcttctctc tataaatcca acttttttat
      481 aaacttttct aagagttttt ctagcttgtt tttcggcttt ttctgctccc atactatata
      541 ttttttctaa ataatcttta ttattaagaa ggtcaacata tttttctctt ataggtctta
      601 aagcttctga tactacttca gccacatctt ctttaaatat gccataaccc tttccttcat
      661 acatgtttac aatctcttct acactctttt tacttaaatt agaatatata tcaattagat
      721 ttttaatacc tggttgttca tcattgtatt ttacaacacc taaagaatca gtaacacttc
      781 tttttatctt tctctttata gaatctgaat catctgctaa tagtataaat gcattttcat
      841 ttgaatcgga tttactcatt tttttagttg gctcttgcaa actcatcact cttgctccac
      901 cttttgggta gtatccttct ggaactacaa aagtaggtga aaatctatta ttaaatctat
      961 ttgctaaatc acgagctaat tctaaatgtt gtttttggtc atctccaact ggaactaggt
     1021 cagtttgata caacaatatg tcagcagcca taagaactgg atatgtgaat aaacctgcat
     1081 ttagatttgc ttcactcttt tgtgatttat ctttgaattg agtcattcta cttaattcac
     1141 ccatataagt catagtattc aatacccaca taagttcaac atgctctttg acatgtgatt
     1201 gtataaatat agtatttttt tctgggtcta atccacaagc tatatattga gcaagtaatt
     1261 ctatagtatt ggctcttaaa actttagggt cttgaggtac tgttattgca tgcaaatcaa
     1321 ctatactata ataacaatca taattgtctt gaagttcagt ccagtttttt atagctccta
     1381 aataatttcc taaagtcatt ttgcctgatg gctgtgcacc actaaatata actttttttt
     1441 cactcatttt tttgtacctc cttatatatt tatattgatt ttaacatatt taaaactatg
     1501 tctttagagt tattggcagc atcatgtaca aatacatcat aagtaacatc agcacttcca
     1561 tctgccttat cagacattgc tcttattatt acaaatggag tattatttaa atagcataca
     1621 tgagctatag ctccaccttc catttctcca caatatccac caaaatcatt tactaattct
     1681 tcttttaatt ctttagaatt tataaattta tctcctgtaa ctactcttcc ttttaatact
     1741 ttatgagtct ttacttcttc cacagaagat ttgtaagcag catctataag tctttcatct
     1801 gctttaaata cagaagtctt cattcttggt ataactccct tagggtctcc aaatgctgta
     1861 gtgtctacat catactgtat agcatcagtt gatattacta tatcattaac atcaagtttt
     1921 tcgtttaaag ctcctgcaac acctgtatta acaatagcat caaccttaaa ttcgcttatt
     1981 agtatctggg cacaaagagc agagttaacc ttacctatgc cacactttac taacactaca
     2041 ttcttacctt ccaaaattcc cttataaaat tctaagctag ctttttttat agtctctcta
     2101 atatccatta aatcaactaa aatactaact tcttcatcca ttgcacctat aattcctatt
     2161 acgttcattt ttcctcctaa attttctata ttaaaaaatc cgtcctaaat caataggacg
     2221 gatatatttt ccgtggtacc acctaaattt atatacactc aaaattgaat atatatatct
     2281 taatatatgc tataacgtgc atacacgata tcaaatactc tgtaatatta aattacattt
     2341 cattttactc ctcaaagacc cattcaataa atacttttta ttaagttctc agcatcctta
     2401 attctctgtg caaaaatata tttatttact atttcttatc tcaggattta atttattaat
     2461 ttctttattt tataatattc cttacactat aaattgtcaa ctatatatta taatatccaa
     2521 aagaaatttt taaaatacat tttatttagc tgttttcttt tcatatctat tccataacca
     2581 tagaccagat aatataacta gaagacctcc accaatcatc tctatctcat atataacatt
     2641 ttcagcaaat gtaggcataa ctggtgctgc agataataat atacccacta cagttacaac
     2701 taaaaccatt tttgctaaac ctaggcaagt acttgttgat tttgccatag tataaggtct
     2761 aatttcatct ggtctctctt ttcttagttt aatataagaa gcatataaca atacatatgg
     2821 gaataatgca gttaatgctg tcatagttac aagaacattg tatatagcat caacagaagg
     2881 cattaatgta gtcaccaata gtattatagt aactattatt gcttgaagta taactgcttt
     2941 agaaggtatc ttgtgttcat ttacctctac taaactatct ggaaatattc cttttttaac
     3001 actaccaaat aacattttta ttggtgatgc tatgtaaaga actattgctc ctaatactga
     3061 aaaagctata ccaaaagcta ttaattgaac caaccaagaa ccaattccta aagacgcaca
     3121 aacagcagaa agtgcatcta atacaccttt agaagcagta atttggtctg gtggtattat
     3181 cattgttatt gcaattgaac ctaatacata taatgaagct actattccag ctgatattaa
     3241 tattgcctta gggaagttct tttcaggctt atctatttct gttatgaagt tagcagcagt
     3301 ctctgctcct gctaaaccaa acattatagc tgatatagca acaaatgcat ccatattaag
     3361 ttttggagtc attgaagcta ttgtgtatgt agaagctata tcatgttttt tcaacacaaa
     3421 tactgctaca aatgaaaata ttataagtat agcacttggt attgttgaac ctaatgcacc
     3481 tgtattagta aatatttttg taaaacttat tcctttagtt gctataagag ataatgccca
     3541 gaatattatt aaagataata taagtacaaa catcttatta tcagcaaggg ctggatttcc
     3601 tattgtataa gaaacattta ctgctaagaa agttaaaaat gaagaatacc aaaatatttt
     3661 agctgtccag tttaaccaag aaactaaaaa tccatacttt tctccaaagg cttccttaac
     3721 ccaatcgtaa agacctccat cacctttttg atatgtagaa gctaattcag cacaaattaa
     3781 agctcctggt acaaagtata taaatgagaa taataaccac ataggtattg cacctaatcc
     3841 taaaccaaag cttgatgctg tagatttagc tatccatctt ataccatata aagctgatac
     3901 attcataaat accaaactcc ataggcctat ttttttcttt tccatcctaa tttaatttcc
     3961 cctctctatt aattatttta aatttaattt caaaattaat aaaatagatt cattattatt
     4021 aaactgatat ttttcaaaat atttgcatat tcaaaaacag ttttaataat aaaccttaaa
     4081 tactttgaaa tcaaatctta ttaaaaatga taaatttaaa agttttacca ctttaattct
     4141 aatgatacta aatttttaaa attgtttcaa tattatttta ttataaaatt ttatttatta
     4201 tttttcatta gtatataaaa agataaaata cataacttta tagacttaat ttattacttt
     4261 tatagaattt tcacaataaa aagtcatttt acttccgtat ttaatgcaaa agtagaaatt
     4321 aaacttaata taaaaaatat ataacaaatt ttttttatat ttctagaaga aaagtatact
     4381 cttttttgtt taataatgct aaaattgatt tgtattgaat tttgattagg aggaaattat
     4441 tatgaaagct caaagattaa atgctgtact tgaacaaatg aaaaaagatg atatttctca
     4501 aatgttagta agtgacccta cttctatatt ttatcttaca ggagtactaa tccatccagg
     4561 cgaaagacta ttagcattat acttaaattt aaatggcaat aataaattat ttataaatga
     4621 attattccca gtttcagaag atttaggtgt tgaaatggtt tggtttaatg atactcaaaa
     4681 tccagtagaa ataataactg aacatataga caaaaatgct actatgggtg ttgataaaaa
     4741 ctggccagct agattcttat taaacttaat agaattaggt ggaggaagta agtttgtaaa
     4801 ttcatcttat ataatagata ctttaagaat gtgtaaagat gaagaagaaa aagagttaat
     4861 gagaatagct tcaaaattaa atgacaaggc tatggaacaa cttaaagcta ctgtttctgg
     4921 tgaacttaca gaaaaacaac ttgttggtaa attatctaaa atatatgaag acctaggtac
     4981 tgatggattt tcatttgacc ctattatagg ttttggacct aatggagcta atccacatgg
     5041 tgaacctgga aatgcacttg tgaaaccagg agatgcaata atcttagata taggttgtat
     5101 aaaagataac tattgtgctg atatgacaag aactgtattc tataaagaaa tacctgaaaa
     5161 aggtagagaa atatttgaaa tagttcttga agctaataag agagctgaag ctattgtaaa
     5221 accaggtgtt agattctgtg atatagatgc tgctgctcgt gactacataa ctgaaaaggg
     5281 atatggacag tacttcactc atagaactgg tcactctata ggacttgaag ttcatgataa
     5341 aggagatgta tcttctataa atacagatac tgtacaacca ggaatgatat tctctataga
     5401 gccaggtata tatttaccag gtgagttcgg agttagaata gaagatttag tacttgttac
     5461 tgaagatggt tgtgagatat taaacaaaca tgacaaagaa atatgtgtag ttggttaatt
     5521 ctaaaatcaa tttgataaaa ttaaaaagcc atataaaaat gttttaaaca tttttatatg
     5581 gctttaattt ctcaatctat gatttatttc aatccattat ttatattgca gttattaaga
     5641 ttgcaccctt tgaagtatat gtccccatca ctgaaccaat ttcacagatt acaacatctt
     5701 tgtattttac atccttttcc ataacttccc ttaccttttg agccttttct gggcaattag
     5761 catgtcctat aaataaaacc ttttcttctt tatctttaat ttttgactct actaaagtaa
     5821 tcagcttttt caaactgttt ccttcacctc ttgccttatc tataggagtt acaacgccat
     5881 ctgcaacttg tattatcgcc ttaaaattta aggtatttat aatttttcct gctattggat
     5941 ttattctacc acccttaata gcattttcta aagtttcaag tgttccaaaa aatggaggat
     6001 tatctttata tttatttatt attgaaacaa tctcttcaat ttcttttcct tcttttatta
     6061 atattgctgc ttttaacact aattgtccta ttccaattga cccactaagt gaatctataa
     6121 cctcaacttt cttgttattt ccttcctttt caaacatatc ttttgctaaa agtgcactat
     6181 tataagtccc agataatcct gaacttatat taatcactaa tacactttct tcctgacaat
     6241 ggtatgcttc taaaaattta tcaggagaag gacatgatgt ttttggtagt actttactct
     6301 cactcatttt tctatagaaa gtttcattat ctatttcctt tccagatata taactttctt
     6361 ctccaaaaga tacatttaaa ccaacaactt ctatgttgta tttttctatt atatcatcag
     6421 gtaagtcaca tgcactgtcc actatcaatt ttattttatc catattaacc tcactataag
     6481 atattatttt atttattcag tcggtcgtac gaccgattta attattattt tatatattga
     6541 atgaaaaaaa gtcaatagat atagattctc gctttattga caataaatat ccataagctt
     6601 ataatgttat taatttgtta taaatataca tggaaaggta ggttttatga aaacaaataa
     6661 tatgaaacag gctattttat catctgcaac caatttaata gccaaaaatg gagttcaaaa
     6721 tactagcctt gctgatattg caaaagatgt aaatataagt aaaggtactt tgtattatca
     6781 ctacgcatct aaagatgata ttatatatga tattgcggat actcatttag aagttataac
     6841 tagtgctata ctaaactgtg taaaaaatgt caaatcaaaa aattctcaaa tagaaatggt
     6901 taacttaatc ttagagaaaa tatctactat agaaagtcgt ggtagggtac atatgtatct
     6961 tatttgtgag gcaattactt caaataatga tttaaaggaa agaataagat taaaatatat
     7021 tgaatggaga actactctta aatctgaaat tgttgaatca ctagaaaaat ataatagtag
     7081 cgaaaatgaa caggatgctg aatcattttc ttttttactc atgtcaatag ttgatggttt
     7141 agttgtccaa agtttattaa aaacagaaaa aataccttat gaaaatatag cttctttttt
     7201 agttaatcat tggatttaaa cttaatactt agataattaa taaaagtgaa ctatttaagt
     7261 tatctacttt aacagttcac tttcttattt attctcaata atttttactt aaaacattca
     7321 atttttattt aaaccttcat ggaatcacaa aataaaaatc tcttataaat ttaaaatgtt
     7381 tttatttatg ttcatgtgta tgcccatctg aatgttcatg accatgtgta tgtgtatgct
     7441 catggctatg ctcatgagta cctgaaaaac catcatcatg attatggtcg tgacctacac
     7501 catttacatg ctcatgttca tgcccatgac tgtgagaatg ttgatgtgaa tgttcatggt
     7561 tatctcctcc acagcatcct tctcctccag tcccacaaca cccacttgta tgtggattat
     7621 gatgatttct tactacaaat ctctctctta acataattct tatacctcct agtatattat
     7681 tgagtacttc atgtacccca ttaactacag tatacatcta actaggtata atctcaatac
     7741 atatggcatt ttttaacata tataattaac atatataatc ttaaagaaat ttatccttaa
     7801 aacctccacc ttttacttct tcaacgttat tgacagaaat aaaagcttca ctatccatat
     7861 cataaattaa atctttaaat ttacctattt catttgcaga tattatgcaa taaatcatct
     7921 ttttcttatt ttgtgtatat gcaccttctg cctccaaaaa agtgactcct ctacccattt
     7981 tattcattat cactttggat atttcatcat atttatttga tattaaaagt atagattttt
     8041 ttctatcaaa gcaatctttt cctatatcca tagtaaaaga aattatatac attgtaatta
     8101 aagtgtacat caccaattta gctccaaata aaaaagctcc tgcacatact acaaaaaaat
     8161 taataaataa aaatgtattt ttcataggta tatcatactt tcttttcatt atggctgcta
     8221 ttatatcaag tcctccagca gtagctcttg ctttaaaagt taaccccata cctataccag
     8281 ttaatgcacc accataaata cactgtagca tagtatcatc tattgggaaa tatttagcaa
     8341 tatcttgtgt tagccccagt gctagagaaa atagtaacat attgacaaaa cttattagca
     8401 aaaatttatt atcaaaatac tttctagcta taataaatat tggtatgttc atcaagaaac
     8461 ttgatattcc ctgattaata ccaaataatt tgtataatat aacacaaatt ccagctactc
     8521 ctccacttaa taacttggct ggtgctagat ataaatttat tcctaaggtt gatagaatca
     8581 tacccaatat aataagacaa tattgtaaaa atatattatt atcaatactt agctttttac
     8641 tttcaccata catcaaaacg cacccctttg cataataaat aaaccaccct gcataacaca
     8701 cgataaattt acaaataaat ttatcagaag tagtttccta aaaatattat tcatcctcac
     8761 ttaaacgttt attcattaat attctatcac atgacataaa taatttatat gatttatata
     8821 gatttattta ttgtaaattt tggaatatta aaacatttta ttgaatagat aattatatta
     8881 cataaaaaat ccctatagaa atctataaac taatttctat agggattttg gttaactaac
     8941 tattattttt atttttttat aacttttata cctgtcatat ggtcatttaa gttttgttcc
     9001 tctaaagatt tatcatctac tctgtttatt tctaaagcta aagtttcaga cttgatatat
     9061 tcattaaaat ggtcaacagc cttagcaatt tcatcatcac cacagtattc tattactata
     9121 ttatctaata cttcaaagtt attagatttt cttaattgct gtactttaga tataaattct
     9181 ctagcataac cttcattaat taactcttca gttagcttag tatctagtat aacaaaaaga
     9241 ttgttttcaa cagatacatt aaatccttcc ttagcagtta tacctattaa tactaaatcc
     9301 ttattaaatt caaaagcttc accatctaaa tcaactgtta aagtctcacc tttttcaagt
     9361 ttattagcag tttcatgagc atttaattta cttaatgctc caacgaataa attcatctta
     9421 gaacctaata atggccctgc ttccttaaag tttggtttta aactaaagtt catatactca
     9481 tctaaatcct tagcaaatat tacctcttta acattaagct cttcttttat taaatcaact
     9541 acatcactta ttgtatcttc aaacttacca tctactaaca ctttttgtaa tggttgacga
     9601 acttttattc tttctacttc tcttgaagct ctacctaaag taactaagtt tttagctaaa
     9661 tccatttttt cttctaattt agtatctact aactcttcat tacatttagg atattcagct
     9721 aaatgaacag atacttctcc tgttaagttt ctatacattt cttctgacat atatggtgca
     9781 aatggagcta tagctctaca aagaccatgt aacaattcat aagttgtatt gtacactgct
     9841 tttttatctt ctgttaattc agttgcccag aatcttcttc ttgaacgtct tatataccag
     9901 ttagataaat catcatttat aaaatcttgt atcattctta aagttttatt taactcaaat
     9961 atagctaaat tttcttctac atctttcatt aaattattga acttagataa tatccatcta
    10021 tcaagttctg gtctatcttt atattctaca aaaaattcag ttggatttat atcatctgtg
    10081 tttgcatata aagtaaagaa gttatataca ttcttcatag tacctataaa tttactttgg
    10141 atttctttta atccatccat atcaaattta gttggagtcc atggtggaga aacatatagt
    10201 aaataccatc ttaatgcatc tgctccatat tggtcaaata attccattgg atttactgta
    10261 tttcctcttg atttactcat tttcttacca tctttatcaa gtacaagatc tggaactaaa
    10321 acactcttat atggtgcttt tcctaataca aatgttgata ctgcaagtaa tgagtagaac
    10381 caacctctag tctggtctat accttctgat atatagtcag ctgggaacaa ttggtcaaag
    10441 ttttccttat tttcaaatgg atagtggtgt tgagcaaatg gcatagcccc actatcaaac
    10501 caacagtcta taacctctgt tactcttgtc attggttttc cacatttttc acattttaag
    10561 tgtatatcat ctacatatgg tctatgaagt tcaactgttt ctggatttac atcttctata
    10621 gctttttcag ctaattcagc tcttgaacca actgaatctg tatgaccaca ttcacatctc
    10681 catataggaa gtggagttcc ccaatatcta gttcttgata ttgcccagtc atttagattt
    10741 tctaaccagt ttccaaatct cttctctcct acaaaacttg ggaaccattt tacaccatta
    10801 ttattttcta ttaatttatc tttaagttta gtcatctcta tataccagct tggttttgca
    10861 tagtatacta atggagtttg acatctccag cagtgtggat aattatgaac tactttttct
    10921 ttgctatata atttattttc agaagctagc cattttatta tttcaacatc tagcccatct
    10981 tccattacaa atctaccttc ccaaggagta gatgtaaatt taccagtttc atctactggt
    11041 tgaataactg gtaaatcata tcttttacct gtgttgtagt catcttcacc aaatgctggt
    11101 gctgtatgaa ctataccagt tccatcttca gtagttacat aatctcctac tgtaataaaa
    11161 aatgcttttt tgtcaacttc tacaaaaggc attaattgtt catattctaa accttctagg
    11221 tctttacctt tcatttcttc taaaacttca taatcttcac ctagtacttt atcagctaaa
    11281 ggtttagata cataatatac ctcatcacct tttttaactt ttaaataatc tacatcagca
    11341 tttactgtta atgaaacgtt tgatggtaaa gtccatggag ttgttgtcca tgctaagaag
    11401 tattcatcag catctttttt cttaaattta gctataactg tattagtttt tacttcttta
    11461 tatccttgtg caacctcatg actagcaaga ccagttccac atcttgggca ataaggaagt
    11521 attttatgtc cttcatatat atatccttct ttgttgaatt tgtttagtat ccaccataca
    11581 gactctatat agttattatc taaagttata tatggattat ctaaatcaac ttcataagcc
    11641 attctctcag tcattattct ccattgtttt tcaaatgaga aaactgactc tctacatttt
    11701 tggttaaatt cagctattcc atattcttct atttgttgct tgctagtaag acctaactct
    11761 ttctctactt gtatttcaac tggtaatcca tgtgtatccc aaccagcttt tctctttact
    11821 tgataaccag acattgtttt atatctacaa actgaatctt taagcgttct agataaaaca
    11881 tggtgtactc caggatttcc attagctgtt ggaggtcctt catagaatac gaaacttggg
    11941 tcatctttcc ctttttctaa agttttttca agtatattta tgtctttcca ataatcaaaa
    12001 acttgtgctt ctgcttgttt tacagaactg tctaccaatg gcttaaattt cgccatgtaa
    12061 atcacccttt cctactaaat attataattc caaaattttt tattctttca cataaattta
    12121 aatactaaaa aactcgcccc ctttaaacca aagggacgag ttataaatcg cgttaccacc
    12181 ctaattctat atgtaaacta aatactagct acatataaca ctctaatagt ataacggtac
    12241 taaccggcac agcttagtag aatttaatcg ttcagcctgc agctcaagag tgatttttat
    12301 ctttataaat tcattggttc tcagcatacc caattctctg taaaattatc taaagataaa
    12361 tgtctctatc aacgctttta aaaatttatc tgtgtttata tgaaattcta taacaaataa
    12421 tttgaatagt caatatattt ttgaaaaata taataatttc tatttttctt acatttttat
    12481 cctattttta atctttgtta tagatttctc aaactcacac aattattcta cataaaaaat
    12541 aaacaaatta caagcttatt ctaaagttgt agcagcttca tcattactat aattatacac
    12601 tgttgttcct tcaaatgcat ttacacattt ttcatctaca tcataaaaaa tctcatcaac
    12661 acttcttatt tcatcttcta acagtgattt aaatttattt ctaaatatct taaattcttt
    12721 gactaaagca tcatactctt ttcttatctc tataacacgg ttattagctt gttctataat
    12781 ttgtctagct tttaaatcag cttcttcaac aattatctta gcctttttat tagccgcgct
    12841 acaagtgtct tctgctgcac tttgagcagt tattaaagta gcttttagtg tttcttcaat
    12901 attttcatac ttgtttactt ggtcttgata taaccctaat ttttctttaa gagctgtatt
    12961 ttccctacat agagattcat aatcttcctt gatgatatct aaaaattcat caacctcttc
    13021 atctctatac cctcttaaac cttttttaaa ctctttattt tctatctcaa ttggagttag
    13081 cattacattt cctcccagct ataatttaaa ttagtcctta tacaattaat cttgcttgaa
    13141 cttttatttt tcctttttta gtaatatctc ctatttgtgt taccttagct cttcctttgc
    13201 ctctaataga aatcaaatca tcgtttttta ctattttaga agttgaagtt atcttttcat
    13261 aattgacatg gactttttct ccatttatgt attttgcact atcttgtcta gatatattgt
    13321 aaataccact tatcacacaa tctaacctat ctgaagatac tgtaaatgat acttctttat
    13381 attttgaaga actattaaca atattttctc tggaaatttc ctttacaatt acattatttc
    13441 ttgaaacctt ttctaagttt acaattataa aatcgcaaat atcactactt acaactactt
    13501 gacaaaagct atcatgtata attatatctc caattttttc tctttttata cctagaccca
    13561 gtattgaccc taagtaatct ttatgagata tatttttaaa tttgaaattc ccttctattt
    13621 gtaaaaatct aagagtatct tctatatctt catattccat gtaaaatgga taaataaata
    13681 cagtgcttct ttcagcttgt tcataacctc catccacact gtattttatt tcatttgtag
    13741 aatttaatat tgctacagca tttttcactt catatggatt aagaaactcc gtagacttaa
    13801 catcatagtt ttttatacaa ctattagcct tgtctatcac tttaaacatt ttatttttca
    13861 agtctatatc ctttatgtga tttgttagtt ttaatttatc catcctatca ccttagaata
    13921 taaccaatac taattgtctt aggaacatca ataatactat tgcaattaaa ggagaaaaat
    13981 ctataggtcc atttgtatat ttatacatta tagaccttat tggcccttct attggctctg
    14041 taagattttc caaaatatca aaaattttac tttgtcttcc atttggaaac caagtcatca
    14101 agctttttat aactatcatc catgctatta tatctaaaag ataataaagt gcaattctta
    14161 ttgttcccat atattaaccg cctttttact atttattttg ccaagggaag aatgctttac
    14221 tttctaattc ttttttcata tttgcatcta tatccacatt attaggagct agtataaata
    14281 ttgatttaga aaccttttgt atacttccat ctaagacata aactgcccca ttcataaagt
    14341 taaatataga tttacgaact tcagaattag tcaatccttc caaattcact ataactgccc
    14401 ttctttgttt tagatggtct gctatagttg cagcttcatc ataaacctta ggttcaacta
    14461 ttacaacttt catttggcta gttgtatgta aatttacaat tttatttgct tttgcagtac
    14521 taaagccact gttcatttct tcttcttgaa ctatatcgtc catgccactt tcatattcat
    14581 cttcaatgta atcttcttct tcatcaacta tccaattctt aaatttagat attattccat
    14641 ttgacattat taacttcctc cttaaaattt attcaaattt gagtaattat tttttattgt
    14701 agtttctttc tccaaagatt gatgttccaa ctctaacaat ggtagcccct tcctctatag
    14761 caatttcata atcgtgactc attcccattg acaaaacatc catccctaca ttagggatgt
    14821 ttagttcttt tatttgtttt gataaatctt taagaccttt aaatacacct cttatctcat
    14881 cttcatcttc tataaaaggt gccattgtca ttagaccctt tatttttata tttttatatt
    14941 tttctgacac ttctttgaca aagtctacaa cttcttctct atcaagtcca tgcttagact
    15001 cttctttaga tatattcact tgtactaagc aatctatttc tctgtttatt tgttctgcac
    15061 gtttttgaat ttcttcgcat aatgcaattc tatctaaaga gtgtatcata caaactttat
    15121 cgattatgta cttaacttta ttggtttgca aagttcctat caggtgccag tttaatttat
    15181 cacctataat atcatacttt ctagctaatt cttggggttt attttcacca acagaagtga
    15241 ttccttccct cattgcctca ttaactaagt ctacatcaac agtctttgtg actgccaaaa
    15301 gggtaatctc tttgctgtct ctatctactt tttttgcagc tttatcaatt ttgtttctta
    15361 tctcagacaa attatcttta atagaaccca ttattatcac ctactttatc cttatatttg
    15421 tgtttatact gttaggtctt aaaattattt tgtcatatag attaacactt gtaactccat
    15481 ttatgtcatt tttataataa tccacaaata cagaagattc attctcataa gttattcctt
    15541 ctaatatgac aaattttaca ctttgtgatt gttcatcaat tacatataca ccacgtttat
    15601 tgtcttttgt ttttatagaa gttttaggta tttttaaaca ttccatctga ttatatatta
    15661 tatcaaattc ttcgacactt gtatcctgat ttgcaacaat ttggttactt atttttaatt
    15721 ttaccattat tttattatta ttttcatata ttttgtaaac ttttgcatct agtttttgct
    15781 catgatattc aattattaca ttttgatttt tttcaaagtt attagaatct tctttactga
    15841 tgtatgtaga tagataatta ctttggtcat tggcatcttt ttctatagtt ccttttacat
    15901 gaatctttgc atcaactttt tcatttttta acaccaacgt atctacattt ttgctaatta
    15961 tcattactaa tatattaaat actaaatata taagaatacc aagtatcact aatctggtat
    16021 attttacctt tttcaaagac tctcccccta taaaattact ttcattataa gtacttcttc
    16081 taaaaaatgt ttattccttt tttataagat aatattttac gttctaataa ttattttttc
    16141 catattaaat ttatttttta tcaatcttcc tatacaagac tgcattattt atatataatt
    16201 gtattataaa gtataagttt aatttacatt ttatacgtta agcttacttt atatattttt
    16261 atagttattt ttatttttag atgcaatata aaaccaatta agatttaaaa taacttttgt
    16321 tatgttgaca tttatttagg taatcattta tgaattagga taacgttatc acaacaaaag
    16381 tttatgtcct aaaaaaaata caaggaggat tttcttatgt cagaaatttt attaggaaca
    16441 ggtttacttt tattggttct atgttgtttc acaattttta gttacaaggc cccatatggt
    16501 atgaaggcta tgggagctct agcaaacgct gcatgtgcta ctttcttagt agaggcattc
    16561 aacctttctt tacttggtga tgttttaggt atggaatttt ttagaagtgt tggagctgct
    16621 aatggtagtc ttggtggagt tgctgctgct atacttgtgc ctttagcctt aggtgtatct
    16681 ccagtttatg cagttttaat aggattatct tgtttagatt tttctatatt atctggattc
    16741 gttgctggat actttgtttc tttcttaatt aagtttatag agaaaaaagt gccacctgga
    16801 gtggatttag tggttgtaat tcttctatgt gcaccactat ctcgtcttat agcatcaaat
    16861 gtagacccat tagttaatag tacactacta caaattgggc aagttttgat agaagctacc
    16921 aacattagcc ctataataat gggaattata ctaggaggtc taataacagt agttggaacg
    16981 tctccactta gttcaatggc tcttacatct ataattggtc ttactggtct tcctatggca
    17041 ataggagcat taggtgcttt tggttcagct tttatgaact caacactatt tgcaaaattg
    17101 aaattaggtt ctaaaaaaga tattatatct gttgctattg aaccattaac ccaatcagac
    17161 ataatttcag ctaatccaat acctatatat tctactaatt tctttggagg agccttttct
    17221 ggtatcattg tagccttgat gggtcttgta aacatgacac caggtacagc tacacctgtg
    17281 gctggttttg ctataatgtt tgcttataat ccagttcaac aagttcttat aacagctggt
    17341 ttatgtgctt tattcaatgt aattgcagga tttttaggtt caaaaatatt cagtaccttt
    17401 aaagttctta ctgctggtga aatacgtggt actgatgtag aagaagaaat tgcttaaaaa
    17461 acaataagat ttataaattt atatctcaag tagtagagtt ttatattgta ggcaatttta
    17521 ttaattgcaa acgttttatt ttttatttat gtttatgatt attatgtgtg ttgaatattt
    17581 agataaattt ttatttatta tttttattta gatttttgtt tagtttttta tttggttgat
    17641 atattaggaa taaaaattag tatagtttat tactagttag atggcataac tttttagata
    17701 cataaaaaat tttatattaa ttagatatta ttaaaataaa agcgatacat gaatttttaa
    17761 atgtccatgt atcgctttat ttttgccata atcttaataa ctgattttta aagtttatta
    17821 tcttaacaaa gttcattatc ttatataata tttaaatgca taggcttctt taatttaacc
    17881 atctaaatat ttaattaaat attattatat gtaatataac taatttttaa atgaatgtat
    17941 aggagctgga atccttcctc ctctttgagt aaataattca gatgaatttg tatttatagg
    18001 cattactggt gctcgtccta aaagtcctcc aaattctacc atatcaccta catcacatcc
    18061 tggggctggt ataattctaa cggctgtagt cttattatta atcataccta ttgctgcctc
    18121 atcagctatc atggctccaa gcgtacctgc tgtagtattt cctggcacag ctatcatatc
    18181 aagacctaca gaacaaatag cagtcattgc ttcaagctta tctatactta aagcaccttt
    18241 aataacagca tcaatcatac ctgcatcttc agatactggt ataaacgctc cactaagtcc
    18301 tccaacatgt gaacatgcca ttactccacc ttttttaaca gcatcattta aaagtgctaa
    18361 tgctgctgta gtcccatgag taccaacaac ttcaagaccc atttcctcta atatatttgc
    18421 tactgaatca ccaatagcag gtgtaggagc tagtgataag tctattatcc caaatggaac
    18481 atccaatcta ctagaagctt cctttgccac aagttgtcca actcttgtaa ttttaaatgc
    18541 agtcttttta actgtttctg aaacaacatc aaaaggctct ccctttactt tctctaaagc
    18601 tctcttaaca acaccagggc cacttacacc aacatttata attctatcag cctcaccaac
    18661 accatggaaa gctcctgcca taaatggatt atcttctact gcattacaaa atactactaa
    18721 ttttgcacaa ccaaaacctt ttgcatcttt tgttaattct gcagtttctt ttatgatttc
    18781 tcccatttct ttcactgcat ccatatttat accacatctt gtagaaccta catttactga
    18841 agcacatact ttattagtac ttgctagagc ttttggaatt gacttaatta gtatcttatc
    18901 accttttgta tatccctttt gaactaatgc tgagaatcca ccaataaaat ctatacctaa
    18961 atcctctgca gccttatcca atgtctgtgc aaactttaca tagtcatttt catctgttgc
    19021 acctgcaata atggaaattg gagttactga taccctttta tttacaattg ggataccaaa
    19081 ttcacgctct atatcttttc caaccttcac caaatcttta gcagaactca taatcttatt
    19141 gtatattttt tctctagctt tatctccatc agggtctata caatctaata aagaaatccc
    19201 catagttata gttcttatat ccaactgttc ttcatctatc atctttattg tttcaagtat
    19261 atttcctaca ttcactatta tcacctcccc ttactgtcta ctacagatta tgcattgaat
    19321 taaatatttc ttctcgctga atttttacat ccactgataa tttcttacct ctttcaacca
    19381 agtcctcaac cgttttctca aaagaattta atgatttctc taaatcaact atcataatca
    19441 tagtaaaata atcctgtaat attttttgtg atatatctac tatgttgata ttttgcttgt
    19501 ataattcatc actaattcct gccactatac ctggtttatc ctttccaatt actgttaaaa
    19561 ttgctttcat aataatatcc tcccttattt ttttataaat cttatatttt tacaaattga
    19621 actatgtttt aacatatatt tcatttattt gttagtaaac cactttttat gtatatattt
    19681 aatatatttt aatacctata cattcatttg tcaatttttt ttcaatattc tattattttt
    19741 atactattta gaaaattcat atattatata tgttttctca acaataaata cagttttca
//
LOCUS       DAYAPA010000071        19542 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.071, whole genome shotgun sequence.
ACCESSION   DAYAPA010000071 DAYAPA010000000
VERSION     DAYAPA010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19542
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.071"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            129..746
                     /locus_tag="ACGI59_002344"
     CDS             129..746
                     /locus_tag="ACGI59_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HFG9740129.1"
                     /translation="MEIFAIGHSNYPYDKLINMIKKYDINCVVDIRETPYSKYNIQYN
                     KEAFNESLRNSGFLYIYMGKEFGAKRTNKDVYTQEGYADFEKVAKEDIFLNGIERLKK
                     GCQMGYRIVLLGAMQEPIRCHRSILVGKVLNKEGFDVKYIMHEGNLAYQEDIEESLLD
                     KYFSDRKQLSIDNLLGSALTREEMIQEGYKLANKEIGYRTEKLGK"
     gene            complement(903..1355)
                     /locus_tag="ACGI59_002345"
     CDS             complement(903..1355)
                     /locus_tag="ACGI59_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staygreen family protein"
                     /protein_id="HFG9740130.1"
                     /translation="MVNLNANNLNVNMVYPISSTAPIRFRRYTVSYLENMENIYLTIA
                     TYFDTLGLGTMNIDKVYGQWAWFTDNIYDLNLFVFVGNYPYQIAKNRYDTFVDILPVA
                     ITSIVNGDRVFLISNPCLLNSKITVRFISSHPTLNRTVDYGNIRDFIF"
     gene            1803..3161
                     /locus_tag="ACGI59_002346"
     CDS             1803..3161
                     /locus_tag="ACGI59_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFG9740131.1"
                     /translation="MFIRKNVIKLAIPIMIEQTFVMLLGVFNTMMAGHIGEEAVSAVG
                     MVDSINNIFISVFAALSVGATVVVAQQIGKKDSEKVNETIKQALVSGVILSFTITILM
                     WVFRTGVINLFYGSAEELVKSNAKLYIEFTLFTYPFIATQQIANGILRGCGDVKKPMY
                     VTMFMNIVNVTLGYILIYGIDLNPLGINLQTPSYGITGAAISIAIARIIGCIVIIRAL
                     FKGNEFISMGKIFPFKIDVETQKSIFNIGIPAGVEQLLFNAGKLIVQVFIVTMGTSAI
                     ASNAIGMSIASIINVIGNALALSATTLVGQYIGRGDIKGAKSTLLYLTKFATICLFIV
                     GAIFIPIAPWISSMYTQNASVIDISTQLIRSDCLAMVIWPISFVLSAGLKGAGDTRYT
                     MMTAVIGMWIFRIFTGYVLGVVLEFGVLGIWIGMYTDWLVRGTLYCLRLRGTKWLKHK
                     VA"
     gene            3434..3814
                     /locus_tag="ACGI59_002347"
     CDS             3434..3814
                     /locus_tag="ACGI59_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFG9740132.1"
                     /translation="MINKIGKITLYVNNQEDAKLFWTEKLNFVVTYEKEMGPNMKWVE
                     VAPSKNEFTTFVLYDKNLMMKQNKEINVSHPSIILSTRDINGTYEQMANNEVEVGELM
                     EMPYGRMFSFKDQDGNDYLVREDK"
     gene            3939..4139
                     /locus_tag="ACGI59_002348"
     CDS             3939..4139
                     /locus_tag="ACGI59_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9740133.1"
                     /translation="MFKKMAVLKDIATKIGRKKAYELLEMVEGNDAFVAEVKIKKNGI
                     ESKKEEIMLKDNQKIILEYIEG"
     gene            4335..5063
                     /locus_tag="ACGI59_002349"
     CDS             4335..5063
                     /locus_tag="ACGI59_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9740134.1"
                     /translation="MLEIKNLSFNVESNNEELGIINDVSLSFERGKLIVITGPNGGGK
                     STIAKLIMGIEKATSGQIILDGEDITNLSITERAKKGIGYAFQQPPRIKGMTVENLLT
                     LAHGKPLSTDVCCQYLTDVGLCSKDYLNREVDNSLSGGEMKRIEIATLFARDLKVSIF
                     DEPEAGIDLWSFGKLNESFKKIHEESNQTIIIISHQERILELADEIIVLQDGSVKSHG
                     TKEAILPEIMCQVNSSCELMKDMN"
     gene            5085..6002
                     /locus_tag="ACGI59_002350"
     CDS             5085..6002
                     /locus_tag="ACGI59_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HFG9740135.1"
                     /translation="MNKIDLNLLEKVAELRGTPLGAYNIRKNGQGVERHSTENVTIIP
                     KTDKPGIDIIVKPNTKGENVHIPVIVTEAGVQDMVYNDFEIGENADVVIIAGCGIHNC
                     GCDDSEHNGIHRFFLRKGARLKYVEKHYGEGDGEGKRILNPVTIVNMDEDSYCEMETI
                     QIKGVDSTKREMDANLGKGAKIVVFEKLLTHGNQVAESNMKVILEGENSSAQVVSRTV
                     GQDNSKQKFNPCVIGDSLCSAHVQCDSIIMGNSQISAIPEIAANHPDALLVHEAAIGK
                     IAGDQILKMMTLGLTEEEAEQQILNCFLK"
     gene            6144..7328
                     /locus_tag="ACGI59_002351"
     CDS             6144..7328
                     /locus_tag="ACGI59_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFG9740136.1"
                     /translation="MKNLLIRNGTIISPTDGIKFEKRDILVLHGKIYAIGLNLKEKIR
                     KEKLLTNKEFEIINADNMYISPGFIDVHTHCYKRKLPTGMDSDSIGIEKGSTVIFDAG
                     TAGPSNYYDFKKKYMDECKTEVYSFLNVTNEGLNILNESTYLDVINFDKVESIVEKNI
                     EKIKGIKVKASKFQSNESGIDIIKKSKQVAKKLNLPLVVYIGEYPSYIDEVMNILGKG
                     DVVTPVYGNSTNGILKESGTLRKSVINAKNRGVLFDVCHGVDQFDFKVFKKAIKQGLE
                     PDLISTDMHIKNMKDVNYSLLNVINKIMELGISLEKCIEKVTEYPTKIFDLHGNKGRI
                     KIGGEGDFTIFTMEECNDIIKDYNNNELVLNKKLRLQYTVKSWMKSSEVYRHGYENTI
                     IN"
     gene            7435..7623
                     /locus_tag="ACGI59_002352"
     CDS             7435..7623
                     /locus_tag="ACGI59_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HFG9740137.1"
                     /translation="MKAFVDKDVCVACGACIGTCPDVFSMSDEGHSVAIEGEIPADLQ
                     DLCKEAEEGCPVSAITVK"
     gene            7772..8422
                     /locus_tag="ACGI59_002353"
     CDS             7772..8422
                     /locus_tag="ACGI59_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFG9740138.1"
                     /translation="MKKNYEIVLFDLDGTLTDPKEGITKSIQYSLNSFGIKEDLENLD
                     QFIGPPLHDTFKEYYKFEDKKAKEAVEKYREYFADKGIFENKIYENMKEILEMLYKNG
                     KILLVATSKPTVFAETILRYFDIDRYFKYIAGSNLDGTRVNKNEVIQYVLDLCNVKDK
                     DKVIMVGDRKYDIIGAKKIGIDSIGVLYGYGSFEEISESEPTYIVENVESIKDILL"
     gene            8525..9463
                     /locus_tag="ACGI59_002354"
     CDS             8525..9463
                     /locus_tag="ACGI59_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFG9740139.1"
                     /translation="MKSILIMGGSDFIGSALAKRLIKCGYQIDILTNGKKEIDYNGFK
                     EHLICDRKVRKDMENIITGRKYDYIYDMTAYTKEDVSNLIDFISMDNLKKYIVLSAGA
                     VYKDSGRNIKEENEKGENENWGKYGLNKKEAEDFIINSPIPYIIIRPTYIYGENNNLY
                     REYYFFEKIEKNEKIPVPKGKQVSNQFIYIGDLVKVLESIMKNPHVREAYNVTNPQLI
                     SWDDLIYTCGEIIGKEPIIKYVDMEKVEFRERTYFPFRNIDFNLDINKLIEHGLYIPN
                     VLLKEGLTATYKWFSANKPKMHDRKMNKVESVMQIV"
     gene            9585..10340
                     /locus_tag="ACGI59_002355"
     CDS             9585..10340
                     /locus_tag="ACGI59_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFG9740140.1"
                     /translation="MKKYNISENGALIKNDSIESKSESVKVVQISDTQIGSFYSTKNL
                     KKVANKINTLNPDIIVFTGDLIDYSNKNPSVDEITTILSSMNARLGKFSVFGNHDYMY
                     KLPRYYRQIMKNSNFNLLVNENKKIKLKDDKYINILGVDEILNGNPNIKYLESQIDNK
                     SFNLLLAHEPDLVDMLSKDTMNLVLSGHSHGGQIRLPIKGALVTPPYGRKYTKGFYDI
                     NGNHLYVSSGLGSTKLPFRFFNIPEIIEFNIII"
     gene            complement(10625..11359)
                     /locus_tag="ACGI59_002356"
     CDS             complement(10625..11359)
                     /locus_tag="ACGI59_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HFG9740141.1"
                     /translation="MSIQKLLSKARKAIQDFDMIQENDKIAVGLSGGKDSLTLLHILK
                     SYQRFSPQNFELIAITLNTGGVDNSPLDKLCKEINVPFYEFQTDIKEIVFDIRQEKNP
                     CALCANLRRGALNDNAKKLGCNKVALGHHKDDAIETFLMSMFYEGRVNCFSPKTYLDR
                     QDLTVIRPMVYIEEYMTKKISKDSNYPIITNPCPANGHTRREYIKNLIANLNKEMPDF
                     KRNVFGALNNSEKLFIWDKEKIKNFK"
     gene            11484..12200
                     /locus_tag="ACGI59_002357"
     CDS             11484..12200
                     /locus_tag="ACGI59_002357"
                     /EC_number="3.4.17.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HFG9740142.1"
                     /translation="MKKFKLIGAIILVVFISVFISKDRLLKKDKDPIVAAQHDSEQDV
                     EANSQNKNHSNSISNILLVNKTNGISKNYTPENITKVNIPFVEEATEEEKQMAGEPAK
                     AVEDLVKQANSEGIQFLGSSAYRSYDTQLDTYTRRVKSQGREKADAYVAKPGYSEHQT
                     GLCIDLTNPERWFVGSTKEAKWLAENAHKFGFIIRYPEGKEDITGTAYEPWHIRYVGK
                     DAAEEIYSKGLTLEEYLQNK"
     gene            12334..13026
                     /locus_tag="ACGI59_002358"
     CDS             12334..13026
                     /locus_tag="ACGI59_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFG9740143.1"
                     /translation="MNNTVLVVDDEKEIRDLIEIYLLNSGYNVIKAEDGKEALDILKK
                     ENIHLMILDIMMPKMDGIEVCRKVRESLNIPILMLSAKSEDMDKIQGIMTGADDYLTK
                     PFNPLELTVRVKSLIRRAYYFSGANNEDKDVIKVGVVIIDKEKHSVTVAGNEIILTSR
                     EFDILYLLANNKGRVYSTEEIFEKVWKEKYYQSNNTVMVHMSRIRDKIEKYTDGEKII
                     HTVWGVGYKIEK"
     gene            13016..14197
                     /locus_tag="ACGI59_002359"
     CDS             13016..14197
                     /locus_tag="ACGI59_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFG9740144.1"
                     /translation="MKNKSLLNHMFRPILDLIISCALTSMLILTSMYIIKSLYVNSDM
                     SVVNQLVYQARDFRSLIGYDLFYPFLFTITSLAIYSMISYKRNKQIVDFIRKTNRKNQ
                     KLEIIKSAVNLMDEGNLEKSIDIEYDLDILKDKEKDDIDELAHKINNIVHQLRNITIE
                     ERQAQQTKTDLITNVSHDLRTPLTSIMGYLGVIEEGKYKDEVQMMYYVDIAYEKSKNL
                     NVLINDLFELTKMQNNTIKLNKIEINLVELLSQVVSQFEIYFKQEFMVSRINFNEEKL
                     IVKADPNKLVRAFENLITNAVKYGKDGHYVDIVTEKKEDMAVVKVINYGEPIPVLDLP
                     NIFDRFYRVEKSRNRNVGGSGLGLAITKNIVNLHDGEITVSSDKYQTVFEVQLPIIQS
                     A"
     gene            14479..14934
                     /locus_tag="ACGI59_002360"
     CDS             14479..14934
                     /locus_tag="ACGI59_002360"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFG9740145.1"
                     /translation="MEKVESFKLDHTKVKAPFVRKCSVLDGVKGDKVTKFDLRFLQPN
                     VESFGTAAMHGLEHLLATYLRDTLDGVIDLSPMGCRTGFYLILWGDVDAKTVKIGLEE
                     ALKKVLESDKMPAATAIECGNYRDLSLFGAKEYAKDVLDKGFSLNIYGE"
     gene            14924..15649
                     /locus_tag="ACGI59_002361"
     CDS             14924..15649
                     /locus_tag="ACGI59_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HFG9740146.1"
                     /translation="MENNLIGREDISINQSEVLRYLQYRGQEIDKDLKYTIDECVAIT
                     KNKINPRYLSRIYPIKINKDNVVELEGTNLTLESKDVYELLKECNECVIMAATIGIDI
                     EKEIRRHSYSNLTKGLIIDSCGTTAIEEVCDIVQNNLEKVFLKQDKFITMRYSPGYGD
                     LPIEKNIDILNVLNAQKQIGLTITSNGIMIPRKSVVAIIGISNTRINKSKKEKSCENC
                     KNYRNCTYRKGANSCGNKTISEK"
     gene            15621..18002
                     /locus_tag="ACGI59_002362"
     CDS             15621..18002
                     /locus_tag="ACGI59_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HFG9740147.1"
                     /translation="MEIRQYLKNNILIFDGAMGTMLQQKGLKLGENPEVFGLQNPDKL
                     IEIHTAYLEAGSNVILTNTFGCNELKLDSKYTVEEVIDNAVLVARKAIENVDNTKPRY
                     VALDIGPIGEMLEPMGTLSFDKAYEIFKRQVLQGVKSGVDVIVIETMMDLYEAKVAVL
                     AAKENSDLPIFCTMTFDEGGRSFTGCMPECMVATIEGLGVDAIGVNCSLGPKQLLPIV
                     EKIASRATVPVMVQANAGLPNIVDGEAIYDVDAKEFFEGVKKFVEVGATIIGGCCGTN
                     PSFIKEISENINSVTKGCIEKIDKCVVCSPSKFVEVQSPTVVGERLNPTGRKSLQEAL
                     KNENVDYAINLGLEQVNAGAQILGVNVGLPEIDEKKLMPKLIREIQAVVDTPLQVDSS
                     NVEALEQGLRYYNGRTIVNSVNGKEESLESILPIVKKYGSCVVGLTLDEKGIPKKAEE
                     RFEIAKRIVNRAVSYGIKPKDIFIDCLSLTVSAQQEEAIETIKAITMVKTLGVKTILG
                     VSNISFGLPNRKALNASFLTLALGAGLDLAIINPNEYSMMEAINSFKILNNTDKGCIN
                     YINQYSNINNSKKSDSSTKIDKDLPLDILVERGLKDEAKNVTLNLLKEKDENYILDEI
                     LIPALDKVGKRYDSGDIFLPQLIQSAETVKVSLNTIKETLLSKSSNNVSKGKIIVATV
                     KGDIHDIGKNIVKIMLENYGYEVIDLGKDVPIEEVVEVAKKRNIKLVGLSALMTTTVQ
                     SMKDTIQALRDNEINAKVFVGGAVLTEEYAEEMGADYYSKDAKSAVEIAKLNF"
     gene            complement(18207..19436)
                     /locus_tag="ACGI59_002363"
     CDS             complement(18207..19436)
                     /locus_tag="ACGI59_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFG9740148.1"
                     /translation="MNLNRNLEKYAELAIKVGVNIQPGQILLIKSPIECADFARNALK
                     EAYKCGAKNVYIEWSDEESTLIKYLYAPDEAFHEFPKWTAEQYVDIAKEGGAFLSVYA
                     QNPDLLKDVDPERVANFQKASGKALKEWRSYTLTDKCKWSIVSVPTKDWAKKVFPNLS
                     EDKAIEKLWDAIFKCSRVDGQDPIKAWEEHNENLKSKMDFLNKNNFKTLKYKSSKTDL
                     TLDLPKGHVWLSGASKDPNGISFNPNIPTEEIFGMPHKFKVNGTVYSTKPLVYGGNII
                     DNFFLTFKDGKIIDFSAEKGLDTLEKLIETDEGSHYLGEVALVPYNSPISNTDIIFYN
                     TLYDENASCHFAIGSAYKTCLEGGNNLKDEELDEHGVNDSLTHVDFMIGSADMDIIGE
                     TYDGKQIQIFKNGNWAF"
BASE COUNT         7819 a         2048 c         3382 g         6293 t
ORIGIN      
        1 aagtaaaaaa atatacaata tcaagaaaaa aagatattgg gtatttaaag taatacttta
       61 tttaatttta agtaaatcgg gtatctacat atatagatac taatttcaaa gaataggagg
      121 gtgtttatat ggaaatattc gcaataggac attcaaatta tccatatgat aagcttatta
      181 atatgataaa aaaatatgac ataaattgtg tagttgacat tagagaaact ccatattcta
      241 aatataatat tcaatacaat aaagaggcat ttaacgaatc tcttagaaat tccggatttt
      301 tatatatata catgggaaaa gaatttggag ctaaaagaac taataaagat gtatatacgc
      361 aagaaggtta tgcagatttt gagaaagtag caaaagaaga tattttttta aacggaatag
      421 aaagattaaa aaagggttgt caaatgggtt ataggatagt tttattagga gctatgcaag
      481 aacctataag atgtcataga agtatcttgg tgggaaaagt attaaataaa gaaggttttg
      541 atgtaaaata tataatgcat gaaggaaatt tggcatatca ggaagatata gaggaatcat
      601 tattggataa gtattttagt gatagaaagc aactgagtat tgacaattta ttagggagtg
      661 cattaacgag agaagaaatg atacaagaag gttataagct agctaataaa gaaataggat
      721 atagaactga gaaactaggt aaataaaaat atacaaatag ataaaataca gtacttatct
      781 taagtaaaat aatattatga atttataata gctaggcatg atgtgccaat taaaaatatt
      841 ctagttattt aataatatca ttaaatttag ggtatttttt aatagcacat catgttgcag
      901 ttttaaaaaa taaaatctct aatattgcca taatcaactg ttctattaag agtaggatga
      961 ctagatataa atctaactgt aatttttgaa tttaacaaac aaggatttga aattaaaaat
     1021 actctatctc cattgacaat agaagtaata gctactggca aaatatctac aaatgtatca
     1081 tatctgtttt tagcaatttg ataaggataa tttcctacaa aaacaaatag atttaaatca
     1141 tatatattat cagtaaacca agcccattga ccataaactt tgtcaatatt cattgtgcct
     1201 aaaccaagtg tatcaaagta tgttgctata gttaaataaa tattttccat gttttctaaa
     1261 tatgaaacag tatatcttct gaaacgtatg ggtgctgtgg aagaaatagg ataaaccata
     1321 tttacattta gattatttgc atttaaatta accaaagtat cacatcctaa aattagtcta
     1381 taaaatacaa tatttaaatg gatgcgattt tgttacatct aaagtgtaat attttatata
     1441 ttgaaaaagt ggtaaaagtt tggatgatta aataatatta taaaaaatat ataaaatgta
     1501 cttattgtta ttgctacacg atagtgatac aatattaacg aaatatagct acttttaata
     1561 ataacggaaa atatttatat taaattaata aatattttaa atgtggtatc cataaaaaat
     1621 aataaacata agataattat aaagatatac tgtgttcaaa tgatattaaa caagatttat
     1681 attatttgaa agcagtatat tataatgcga acaatacaat tctaaacttt gcattgtcaa
     1741 tttacagttc gataaactaa ttaaaaatta aatttatata aagagggaaa agaggaatag
     1801 ggatgtttat aaggaaaaat gttataaagt tagcaattcc tataatgata gagcagacgt
     1861 ttgtcatgct tttaggagta tttaatacaa tgatggcagg tcatattggg gaagaagctg
     1921 tatctgcagt tggaatggtt gattcaataa acaatatatt tatatctgta tttgctgcac
     1981 tatctgttgg agctactgtt gttgtagctc aacaaatagg aaaaaaagat agtgaaaagg
     2041 tgaatgaaac tattaagcag gctctagtgt ctggagtaat attatccttt acaataacaa
     2101 tattgatgtg ggtttttaga actggtgtaa ttaatttatt ttatggttcc gcagaagaat
     2161 tggtaaaatc aaatgcaaaa ttatacatag agtttacatt atttacgtat ccatttattg
     2221 cgacacaaca aatagcaaat ggtattttaa gagggtgtgg agatgtaaag aaacctatgt
     2281 atgtgactat gtttatgaat atagtaaatg taacattggg atatatactt atatatggta
     2341 ttgatttaaa tcctctagga ataaatttgc aaacaccatc ctatggcata acaggtgcag
     2401 ctatatctat tgctatagct agaataattg gatgtatagt aattataaga gctttattta
     2461 aaggaaacga atttatatct atgggtaaaa tatttccatt taagattgat gttgaaactc
     2521 aaaagtctat atttaatata ggaataccag ctggagttga acaactactt tttaatgcag
     2581 gtaaattgat tgttcaagta tttatagtta ctatgggaac atcagcaatt gcatccaatg
     2641 caataggaat gtcaattgca tccataataa atgtaattgg gaatgcttta gctttgtctg
     2701 ctacaacctt ggtgggacag tatataggac gtggagatat taaaggagct aagagtactt
     2761 tattatattt aactaaattt gcaacaattt gtttatttat agtaggagct atatttatac
     2821 caattgcacc ctggatttca agtatgtata ctcaaaatgc tagtgtaatt gacatctcaa
     2881 cacaattaat tcgaagtgat tgtttagcta tggtaatatg gcctatatcc tttgtattat
     2941 cagcagggct aaagggagcc ggtgatacta gatatacaat gatgactgca gtaataggta
     3001 tgtggatttt tagaattttt acaggttacg tattgggggt tgtattagaa tttggagtgt
     3061 taggaatttg gataggtatg tatactgatt ggcttgttag agggacttta tattgtttaa
     3121 gattaagagg aacaaagtgg ctaaaacaca aagttgcata gaatatatga aatcactttt
     3181 agatggataa ctctatttag aagtgatttt ttatgtagtt attatcataa ttataagtat
     3241 gatatataag gtgattaaat tacataaata aaggctataa tagtatggat attataaatt
     3301 atatattgag cagaattaca taatttggcg ataaatatta gaaatatata tctatagaaa
     3361 ataatatata tgggtatatt attaaatata aaaaatatta ggtatttatt taataataaa
     3421 ggtgggtatt gttatgataa ataaaatagg aaaaataact ctctatgtaa ataatcaaga
     3481 ggatgcaaag ttattttgga cagaaaaatt gaactttgta gtaacttatg aaaaggaaat
     3541 gggtccaaat atgaaatggg tggaagtagc accaagtaaa aatgaattta caacatttgt
     3601 gttgtatgat aaaaatctaa tgatgaagca aaacaaagag ataaatgtat ctcatccatc
     3661 aataatttta agtacaagag atattaatgg aacttatgaa caaatggcaa ataacgaggt
     3721 tgaggttggg gaattaatgg agatgccata tggaaggatg ttttctttta aagaccaaga
     3781 tggaaatgat tatttggtca gagaggataa ataattttac aaagtattaa atttactcaa
     3841 tatctgatat aattagagac aataattatt ataaactagt ctaggattag aattatataa
     3901 ttaaaaatta agtgtaaatt ttgagtagag gtgataaaat gtttaagaaa atggcagtac
     3961 taaaagatat agcaactaaa ataggtcgta aaaaagcgta tgaactatta gaaatggttg
     4021 aaggtaatga tgcctttgta gctgaggtaa agataaaaaa gaatggaata gaatctaaaa
     4081 aagaagaaat tatgttaaaa gataatcaaa aaataatatt agagtatata gaaggttaaa
     4141 ttttaaatac atataagttg atatactaaa tttctgagat gagtttacat aaaactcatc
     4201 ttttttactt ttcaataact ttgttttatt atttaatata cattttatct ccttagagtt
     4261 gctgatatat aaaaattact gatataataa aagaatggat attaatgtaa tattttatag
     4321 aaaagagggg tattatgctt gaaataaaaa atctttcctt taatgttgag tctaacaatg
     4381 aggaactagg aataataaat gatgttagcc tttcatttga gaggggaaaa cttattgtta
     4441 tcacaggtcc aaatggtgga ggaaaatcta caattgctaa gcttattatg ggaattgaaa
     4501 aagctacgag tggtcagata attttagatg gagaagatat aacaaatctt agtattacag
     4561 aaagagcaaa aaaaggaata ggatacgcat ttcaacaacc accaagaata aaaggtatga
     4621 cagttgaaaa tttacttaca cttgctcatg gaaaaccatt gtctactgat gtatgttgcc
     4681 aataccttac agatgttgga ttgtgttcaa aagactattt aaatcgtgaa gttgataact
     4741 ctctttctgg aggggaaatg aaaagaattg agatagcaac tctttttgct agagatttaa
     4801 aggtttctat atttgatgaa cctgaagcag ggatagacct ttggagcttt ggaaaactca
     4861 atgaaagttt taagaaaata catgaagagt ctaatcagac tataatcata atttctcacc
     4921 aagaaagaat attagagtta gctgatgaaa taattgttct acaagatggt tcagtaaaaa
     4981 gccatggaac aaaagaggct atattacctg aaatcatgtg tcaagttaat tcaagttgtg
     5041 aattaatgaa agacatgaat taattagaaa ggaaggcaaa ttaaatgaac aaaatagatt
     5101 taaatctttt agaaaaagtt gctgaacttc gtggaactcc attaggagca tacaatatac
     5161 gtaaaaatgg gcaaggagta gagagacatt caactgaaaa tgtaactata attcctaaaa
     5221 ctgataaacc tggaatagat attatagtaa agccaaatac aaaaggagaa aatgtacata
     5281 taccagtaat agtaacagag gctggtgtac aggatatggt atataatgat tttgaaattg
     5341 gagaaaatgc agatgttgtt ataattgctg ggtgtggtat acataactgt ggttgtgatg
     5401 atagtgaaca taatggtata catagatttt tcttacgtaa aggagctcga ctaaaatatg
     5461 tagaaaaaca ttatggtgaa ggtgatggag aaggaaagag aattttaaat ccagttacta
     5521 ttgtcaatat ggatgaggat tcatattgtg aaatggaaac aattcaaatt aagggtgtag
     5581 attcaactaa aagagaaatg gatgcaaacc taggtaaagg agctaaaata gttgtatttg
     5641 agaaattatt aactcatggt aatcaagttg cagaatctaa tatgaaggtt atcttagagg
     5701 gtgaaaactc ttctgctcaa gtagtttctc gtacagttgg gcaagataat tctaagcaaa
     5761 agtttaatcc ttgtgttatt ggggattccc tttgttctgc acatgttcaa tgtgattcaa
     5821 taattatggg aaattctcaa attagtgcta tacctgaaat tgctgcaaat catccagatg
     5881 ctcttttagt acatgaagca gctattggaa agattgcagg agaccaaata ctaaagatga
     5941 tgacattagg tcttactgaa gaagaagccg agcaacaaat acttaattgc ttcttaaagt
     6001 aaattaaaga ttttatttaa tttaatataa ttaatatagt ataatatagt gaataagtct
     6061 aaaatatttt tatttttagg cttattttct ttatgtaaga aaagctatta gagaaattat
     6121 attgtaatta ggatgtggaa taaatgaaaa atttacttat aagaaatggg acaataatat
     6181 ctccaacaga tggtatcaaa tttgaaaaaa gagatatctt ggttttacat gggaaaatat
     6241 atgctattgg attgaacctt aaagagaaaa taagaaaaga aaaattactt acaaataaag
     6301 agttcgagat aataaatgcc gataatatgt atatttcccc aggatttata gatgttcaca
     6361 cccattgtta taagagaaaa ttacctacag gtatggattc tgattcaatt ggaattgaaa
     6421 aaggttctac agttatattt gatgcaggta cagctggtcc ctcaaactac tatgatttta
     6481 aaaaaaagta tatggatgag tgtaaaacag aagtatattc ttttttaaat gttacaaatg
     6541 agggactaaa tatattgaat gaatcaactt atttagatgt tataaatttt gacaaggttg
     6601 agtcaattgt agaaaaaaat atagaaaaga taaagggtat aaaagtaaaa gcgagtaaat
     6661 ttcaatctaa tgaaagtggt atagatataa ttaagaaatc aaagcaagtt gctaaaaaat
     6721 taaatttacc cttagtggta tatataggag agtatccatc atatatagat gaagtaatga
     6781 atattttggg aaaaggagat gtggttacac cggtatatgg aaatagtaca aatggaatcc
     6841 ttaaagaatc tggcacatta agaaaatcag taataaatgc aaagaataga ggagtattgt
     6901 ttgatgtatg tcatggagtg gaccaatttg attttaaagt ttttaaaaaa gcaataaagc
     6961 aaggtttaga gcctgaccta ataagtactg atatgcatat aaaaaacatg aaagacgtaa
     7021 actattctct tttaaatgtt ataaataaaa ttatggaact tgggatatcc ctagaaaagt
     7081 gtatagaaaa agtaactgaa tacccaacaa agattttcga tttacatgga aataaaggca
     7141 gaataaagat tggtggagaa ggtgatttta caatatttac aatggaggag tgtaatgata
     7201 ttataaaaga ctacaataat aatgaacttg ttttaaataa aaaattaaga ttgcaatata
     7261 ctgtaaaatc atggatgaaa agcagtgaag tttatagaca tggatacgaa aatactataa
     7321 taaactaaac aatagtgata aaataataaa tatatgttta aattggtata tattggataa
     7381 atatctaagg aaacaacaat taaaaaataa taattttagg ggaggtattt tagtatgaaa
     7441 gcatttgttg ataaggatgt atgtgtagct tgtggagcat gtataggaac atgtccagac
     7501 gtttttagta tgagcgatga agggcattca gtagcaattg aaggagaaat accagcagat
     7561 ttacaagatt tatgtaaaga agccgaagaa ggatgtccag tttcagctat aacagttaaa
     7621 tagagctgtt taaatttatt tttaaaataa atatataaga tttaattggt acttaagcta
     7681 tagtattaga aatttctatt actatagctt tttaaaatag ataaacttaa tatataagat
     7741 attacttttt acaaatatgg aggactgaaa tatgaaaaaa aattatgaaa tagtattatt
     7801 cgatttagat ggaacactta cagaccctaa agaaggaata acaaagtcta ttcagtattc
     7861 tttaaatagc tttggcataa aagaagattt ggaaaactta gaccaattta taggaccacc
     7921 attacatgat actttcaagg agtattataa atttgaagat aaaaaagcta aagaagctgt
     7981 tgaaaagtat agagaatatt ttgctgataa agggatattt gaaaataaaa tttatgagaa
     8041 tatgaaagag atattagaaa tgctctataa aaatggtaaa atattattag tagcaacttc
     8101 taagccaaca gtttttgcag aaactatact tagatatttt gatatagata gatattttaa
     8161 atatatagca ggtagcaatc ttgatggtac aagagtgaat aaaaatgaag ttatacaata
     8221 tgtactagac ctatgtaatg taaaagataa agataaagtt attatggttg gagatagaaa
     8281 gtatgacata attggagcta aaaaaatagg tatagattct attggagtat tgtatgggta
     8341 tggttctttt gaagagatta gtgaaagtga acctacttat attgtcgaaa atgtagagtc
     8401 tataaaagat attttgttat aaatatcttt tatagacaaa aatattacaa aatggtgtcg
     8461 catatttaaa aatatgatat tatagttaag gaagtcattt aaaaaagagg aggatgcact
     8521 taaaatgaaa tctattttaa ttatgggtgg aagcgacttc ataggttctg cacttgctaa
     8581 gcgcttgatt aaatgtgggt atcaaattga tatacttaca aatggtaaaa aggaaataga
     8641 ttataatggt tttaaagaac atctaatttg tgatagaaaa gttcgaaaag atatggaaaa
     8701 cattatcaca ggaagaaaat atgattacat atatgatatg acagcatata caaaagaaga
     8761 tgtaagtaat ctgattgatt ttataagcat ggataactta aaaaagtata tagtattgtc
     8821 agcaggagca gtatataaag atagtggaag aaatataaaa gaagaaaatg agaaaggtga
     8881 aaatgaaaac tggggaaaat atgggttaaa taaaaaagag gctgaagatt ttattataaa
     8941 tagccctata ccatatataa ttattagacc aacttacata tatggtgaaa ataataattt
     9001 atatagagag tattatttct ttgaaaaaat agaaaaaaat gaaaagatac cagtaccaaa
     9061 aggaaaacaa gttagtaatc aatttatata tattggggat ttggtaaagg tattggaaag
     9121 cataatgaaa aatcctcatg taagagaggc ctataatgtg acaaatccac agttaatttc
     9181 ttgggatgac cttatatata catgtggaga aattattggc aaagaaccta taataaaata
     9241 tgtagatatg gaaaaagtag agtttagaga aagaacgtat ttcccattta gaaatataga
     9301 ctttaatctt gatataaata agcttattga acatgggctt tatataccaa acgtgttact
     9361 aaaagaaggt cttacagcaa catataaatg gttttcagca aataagccaa aaatgcatga
     9421 cagaaaaatg aacaaagttg agtctgtaat gcaaatagtt taaaaaaata aataagtagg
     9481 tagatatttt gaactcaaaa attaagtatt ttttaaaatt ttttatctta atatttattt
     9541 tgatttttat atactcagtt gcgatagagc cgagtctatt aattgtgaaa aaatataata
     9601 taagtgaaaa tggagcattg ataaaaaatg attcaattga gagtaaatct gaaagtgtaa
     9661 aagtagttca aatttcagat actcaaatag gtagttttta ttcaactaag aatctcaaaa
     9721 aagtagctaa taagattaac actttgaatc cagatataat agtatttact ggtgatttaa
     9781 ttgattactc caataaaaat ccaagtgtag atgaaattac aactatttta tcaagtatga
     9841 atgctaggtt gggaaaattt tcagtatttg gcaatcatga ttatatgtat aaacttccta
     9901 ggtattatag gcagattatg aaaaattcca acttcaatct tttagtaaat gaaaataaaa
     9961 aaataaaatt aaaagatgat aaatatatta atatattagg tgttgatgaa atattaaatg
    10021 gaaatcctaa tataaaatat ctggagagtc aaatagataa taaaagtttt aatttattgt
    10081 tagcacatga accagattta gtagatatgc ttagtaaaga tacaatgaat ttagttctat
    10141 ctgggcatag tcatggtggt caaataagat tacctattaa gggagcatta gttactcctc
    10201 catatggaag gaaatatact aaagggtttt atgacataaa tggaaatcat ttatatgtga
    10261 gttcgggtct tggaagtaca aagttaccat ttagattttt taatatacca gagataatag
    10321 aatttaatat tataatctaa cataaaatat ttagaaaata taattttgct ttttataata
    10381 tgtagatgta tatactgtaa aaatggatta aaccagtaga ttgaatgaat taatagatta
    10441 aattaatata taaaatattt aagttataat taatgtatta gacaaaataa atactattaa
    10501 gaactattaa aatatagatt aaagataata acatataata aataaaaaag taagattgtg
    10561 tagattatat aaagattatt tacaattaat acaatcttgc ttttttattt aaaaatttta
    10621 attattattt aaaatttttt atcttctctt tatcccatat aaacaatttc tcagaattat
    10681 ttaaagcacc aaatacattt cttttaaaat caggcatctc tttatttaaa ttagcaataa
    10741 gattttttat gtactctctc ctagtatgac catttgcagg gcagggattt gtgataatag
    10801 gatagttaga atcttttgaa attttcttag tcatatactc ttctatgtaa accataggtc
    10861 ttataactgt taaatcctgt ctgtctaggt atgtctttgg ggaaaaacaa tttactctac
    10921 cctcataaaa catagacatt aaaaatgtct caattgcatc atctttatga tgaccaagag
    10981 ctactttatt acatccaagt tttttagcat tatcgttcaa agcacctctt cttaaattag
    11041 cacacaatgc acaaggattc ttttcttgtc ttatatcaaa tacaatttct tttatgtcag
    11101 tttgaaattc ataaaaagga acatttattt ctttacacaa tttatctagt ggagaattat
    11161 caacacctcc agtatttaat gttatggcta tcaattcaaa gttttgtgga gaaaatcttt
    11221 gataactttt aagtatatga agtagagtta aactatcttt tccaccagaa agtccaaccg
    11281 caattttatc attttcttgt atcatatcaa aatcttgtat agccttacga gctttactta
    11341 gaagtttttg tatagacatt ttatcaccat cctttaaaaa tattaaattt aagatttatt
    11401 taagatttac ttataaaata cttaagtctt atactctaaa attaacatta tagcaaacat
    11461 aacaaaaagg agaagattta attatgaaaa aatttaaatt aataggagcc attattttag
    11521 tagtatttat aagtgtattt atatctaaag atagattact taagaaagat aaagatccaa
    11581 tagtagctgc tcaacatgat tcagaacaag atgttgaggc aaattctcaa aataaaaatc
    11641 actcaaatag tatatcaaat atattattgg taaacaagac aaatggaatt agcaaaaact
    11701 atactcctga aaatattact aaggtaaaca taccttttgt tgaagaagca acagaggaag
    11761 aaaaacaaat ggcaggagaa cctgcaaagg cagttgaaga tttagttaag caagctaatt
    11821 cagaaggaat acagttttta gggtcttcag catacagatc ttatgatact caactggata
    11881 cttatacaag aagggtaaaa tctcaggggc gagaaaaggc agatgcgtat gtagcaaagc
    11941 ctggttacag tgagcaccaa actggacttt gtattgattt aacaaatcca gaaagatggt
    12001 ttgttggaag tacaaaagaa gctaaatggt tggcagaaaa tgctcacaag tttggattta
    12061 taataagata tcctgaaggt aaagaagata taacaggtac agcatatgaa ccatggcata
    12121 ttagatatgt tggtaaggat gctgccgagg aaatttattc aaaaggatta actttagaag
    12181 aatatttaca aaataaataa tataaatgtg taaataattt gtattatatt ggaaatttag
    12241 aaattaataa atttatagat tacactaact aaaatatagt ataattttat atgaataaag
    12301 ctatatacaa agattaagtt aggatgtgta aaaatgaata atacagttct tgttgtggat
    12361 gatgaaaagg aaataagaga ccttatagag atatatcttt taaatagtgg atataatgta
    12421 attaaagctg aagatggaaa agaggctcta gatattttaa aaaaggaaaa tattcattta
    12481 atgatattgg atataatgat gcctaaaatg gatgggatag aagtttgtag aaaagttaga
    12541 gaaagtttga atataccaat acttatgtta agtgcaaaat cagaagacat ggataaaata
    12601 caagggataa tgactggtgc agatgattat ttgacaaaac catttaaccc acttgaactt
    12661 actgtgagag tgaagtcgct gattagaaga gcatattatt ttagtggtgc taataatgaa
    12721 gataaagatg tcataaaggt tggagtagta attatagata aggaaaaaca tagtgtaaca
    12781 gtggctggaa atgaaataat tttgacatca agagaatttg atatattata tttactagca
    12841 aataataaag gcagagtata tagtacagaa gaaatatttg aaaaggtatg gaaagagaaa
    12901 tattaccaat ctaataatac tgtaatggtt catatgagca gaataaggga taaaattgaa
    12961 aaatatacag atggagaaaa aataattcat actgtatggg gagtaggata taaaattgaa
    13021 aaataaaagt ttattaaatc atatgtttcg acccatatta gatcttataa taagctgtgc
    13081 tttaacttca atgcttattt taacaagtat gtatataata aaatcattat atgtcaatag
    13141 tgatatgtct gtagttaatc aacttgtata tcaagcaaga gatttcagaa gtcttatagg
    13201 gtatgattta ttttatccat ttttatttac aatcacatca ttagcaattt attccatgat
    13261 atcgtacaag agaaataagc aaattgtaga ttttataaga aagactaata gaaaaaatca
    13321 aaaattagaa atcattaaaa gtgctgtaaa tttaatggat gaaggtaact tagaaaaaag
    13381 tatagatatt gagtatgatt tagatatatt aaaggataaa gaaaaggatg atattgatga
    13441 actagcacat aaaatcaata atatagtcca tcaacttaga aatataacta ttgaagagag
    13501 acaagctcag cagacaaaaa cagatttaat aacaaatgtg tcacatgact taagaacacc
    13561 tttgacatcc ataatgggtt atttaggagt gatagaagaa ggtaagtata aagatgaagt
    13621 tcagatgatg tattatgttg atattgcata tgaaaaatcg aaaaatttaa atgttcttat
    13681 aaatgatttg tttgaattga ctaaaatgca aaataataca ataaaattaa acaaaataga
    13741 aataaattta gtagagttat taagtcaagt agtatctcaa tttgaaattt attttaaaca
    13801 agaatttatg gttagtagaa ttaattttaa tgaagaaaaa ttaattgtaa aagctgatcc
    13861 aaataaattg gtaagagcat ttgaaaatct tataactaat gctgtgaaat atggaaaaga
    13921 tgggcattat gtagatatag tcacagagaa aaaggaagat atggcggttg taaaagtgat
    13981 aaattatggg gaaccaattc cagtgttaga tttaccaaat atatttgata gattttatag
    14041 agttgaaaaa tcgagaaata gaaatgttgg aggttctgga ttgggtcttg cgattacaaa
    14101 aaatattgta aatttacatg atggagaaat tactgtttca agcgataaat atcaaactgt
    14161 gtttgaagta caactgccaa taatacaatc agcttagtta taaaataaaa gtacgattat
    14221 gtgatataaa tattataaca ataaagtgtt tacaaaaata aaaaagtaat atataattta
    14281 agtcagttta ataagatact tatcaagagt taggggagag agttagctca gtgatcctat
    14341 accaaccttt tatcaggtgg tcctgctaac aacgatgaga tgacttgaaa taatatactc
    14401 ttcttatcaa taatagatag gaagagtttt ttttagaatt aatgtaacaa attttaaagt
    14461 ataatggagg attaaataat ggaaaaagta gaaagtttta agctagacca tacaaaagta
    14521 aaagctcctt ttgtaagaaa gtgtagtgta cttgatggag taaagggaga taaagtaact
    14581 aaatttgact tgagattttt acagccaaat gtagaaagtt ttggaactgc tgctatgcac
    14641 ggtttagagc atcttttagc tacttattta agagatacat tagatggtgt aatagactta
    14701 tctccaatgg gatgtagaac aggattttat ttaatactat ggggagatgt agatgccaaa
    14761 acagttaaga ttggtttaga agaagcatta aaaaaagttc ttgaatcaga taaaatgcca
    14821 gcagctacag ctatagaatg tggaaactac agagacttat ctttatttgg ggcaaaggaa
    14881 tatgcaaaag atgttttaga taaaggattt tctttaaata tatatggaga ataatttaat
    14941 tggaagagaa gatataagca taaatcaaag tgaagtttta agatatttac aatatagagg
    15001 tcaagagata gataaagacc ttaaatatac tattgatgaa tgtgtagcta taactaaaaa
    15061 taaaataaat cctagatatt tatctaggat ttatcctatt aaaataaata aagataatgt
    15121 tgtagaatta gagggaacaa atttaacatt agagagtaaa gatgtatatg aacttttaaa
    15181 agaatgtaat gagtgtgtaa ttatggcagc tactataggg atagatatag aaaaagaaat
    15241 aagacgacac tcttattcaa atttaacaaa aggactaata attgattcat gtggaacaac
    15301 agcaatagaa gaagtgtgtg acattgttca aaataattta gaaaaggttt ttttaaagca
    15361 agataaattt ataactatgc gatatagccc aggatatgga gatttaccaa tagaaaaaaa
    15421 tatagatata ttaaacgttt tgaatgctca aaaacaaata ggtctaacaa ttacttctaa
    15481 tggaattatg ataccgagaa agtcagtagt ggctattata ggtatttcta atacaagaat
    15541 aaataaatct aagaaagaaa agtcgtgtga aaattgtaaa aactatagaa actgtacata
    15601 tagaaaagga gcgaactctt gtggaaataa gacaatatct gaaaaataat atattaatat
    15661 ttgatggtgc aatgggaaca atgcttcaac aaaaagggtt aaagttaggt gaaaatccag
    15721 aagtatttgg tcttcaaaat ccagataaac ttatagaaat acatacagca tacttagagg
    15781 caggttcgaa tgtaattctt acaaatactt ttggatgtaa tgaactaaaa ttagatagta
    15841 agtatactgt agaagaagtg attgacaatg ctgttttggt ggctagaaaa gctatagaaa
    15901 atgtagataa tactaaacca agatatgtag ctttagatat tggtcctata ggagaaatgc
    15961 tagagcctat gggtacttta agttttgata aagcatatga aatatttaaa agacaagtac
    16021 tacaaggcgt taaatctgga gttgatgtaa tagtaataga gactatgatg gatttatatg
    16081 aagctaaagt agctgtatta gcagcaaaag aaaactctga tttacctatt ttctgtacta
    16141 tgacatttga tgaaggtgga agaagtttta ctggatgtat gccagaatgt atggtggcta
    16201 ctatagaagg tctaggtgta gatgcaatag gagttaattg ttcattagga cctaaacaac
    16261 ttttaccaat agttgaaaaa atagcatcaa gagctacagt ccctgttatg gttcaagcaa
    16321 atgcaggtct tccaaatata gttgatggag aggctatata tgatgttgat gcaaaagaat
    16381 tttttgaagg ggttaaaaag ttcgttgaag ttggtgctac tataataggt ggatgttgtg
    16441 gtacgaatcc aagttttata aaagaaatat ctgagaatat aaattctgtt acaaagggat
    16501 gtatcgagaa aattgataaa tgtgtagtct gttcaccatc aaaatttgtg gaagtacaat
    16561 cgcctacagt tgttggagaa agattgaatc ccacaggtag aaaatctcta caagaagcat
    16621 taaaaaatga aaatgttgat tatgctataa atttaggttt agaacaagta aatgctggag
    16681 cacaaatact tggagtaaat gttggtcttc cagagattga tgaaaaaaaa ctaatgccaa
    16741 aattaataag agagattcaa gcagtagtag atacaccttt acaagttgat tcttccaatg
    16801 tagaggcctt agagcaagga cttagatatt ataatggtag aacaatagta aactctgtaa
    16861 atggcaaaga ggagtcttta gaaagtatat taccaatagt taaaaagtat ggttcatgtg
    16921 tggttggact tacacttgat gaaaagggga tacctaaaaa ggctgaagaa agatttgaaa
    16981 tagcaaaaag gatagttaat agagcagttt cttatgggat aaaaccaaaa gatatattta
    17041 tagactgttt atcattaact gtttcagcac aacaagaaga agcaatagaa actataaaag
    17101 ctataacaat ggtaaagaca ctaggagtaa aaactatatt aggtgtttca aatatttcat
    17161 ttggtcttcc aaataggaaa gcacttaatg cttcttttct aacattagct ttaggggcag
    17221 ggcttgattt agcaattata aatccaaatg aatatagtat gatggaagct atcaattctt
    17281 ttaaaatatt aaataacaca gataaaggat gtataaacta tataaatcaa tatagcaata
    17341 taaataattc aaaaaaatcg gattcatcta caaagattga taaagatttg cccttggata
    17401 ttttagttga aagaggatta aaagatgaag caaaaaatgt aacactgaat cttttaaaag
    17461 agaaggatga aaattatata ttagatgaga ttttaattcc tgctcttgat aaagtaggaa
    17521 aaagatatga tagtggagat atatttttac cacaattgat tcaatcagca gaaacggtaa
    17581 aagtttcatt aaatacaata aaggaaactt tattaagtaa aagtagcaat aatgtatcca
    17641 aaggaaaaat aatagttgct acagtaaaag gtgatatcca tgatattggg aaaaatatag
    17701 taaaaataat gctagaaaat tatggatatg aagttataga tttaggtaaa gatgtcccta
    17761 ttgaagaagt agtggaagtt gctaaaaaga gaaatataaa attagtagga ttaagtgcac
    17821 ttatgactac gactgttcaa agtatgaaag atacaattca ggcactgaga gataatgaga
    17881 taaatgcaaa ggtatttgta ggaggtgcag ttttgacaga agaatacgct gaagaaatgg
    17941 gtgctgatta ttattcaaag gatgcaaagt ctgctgttga aatagcaaag ttaaattttt
    18001 agcatataaa ttaaaataaa ggttttacta aataggatat tttatatata atataaatat
    18061 aagttttaga tacttatgtt taaaacaata ggaattaatc tataataatg agattaatta
    18121 atataaaaaa tatttatcat caacattaaa aagggctggg aagtctggta taacttccat
    18181 cagccctttt tagttaaaat catatgttaa aaagcccaat taccattttt aaaaatctgt
    18241 atttgctttc catcataagt ctcaccaata atatccatat cagcagaacc aatcataaaa
    18301 tctacatgtg ttaaactgtc atttacacca tgttcatcta attcttcatc ttttaaattg
    18361 tttccacctt ccagacaagt cttataagca gaaccaattg caaagtggca agaagcattt
    18421 tcatcatata gagtattata aaaaattata tccgtattgg atattggtga gttgtatgga
    18481 acaagagcaa cttctcctaa ataatgagag ccttcatccg tttcaataag tttttctagt
    18541 gtatctagtc ccttttcagc agagaaatct attattttac catctttaaa tgttaagaag
    18601 aaattgtcaa ttatgtttcc accataaaca agaggttttg tactataaac agttccattt
    18661 actttaaact tatgtggcat tccaaaaatc tcttcagttg gaatattcgg attaaaactt
    18721 attccattag ggtctttgga tgctccactt agccaaacat gaccctttgg caagtctaga
    18781 gtcaaatcag tttttgagga tttatatttt aaagttttaa agttattttt atttagaaag
    18841 tccattttag atttaagatt ttcattatgt tcttcccatg cttttatagg gtcctgacca
    18901 tctactcttg aacatttaaa tatagcatcc cacagttttt ctattgcttt atcttctgat
    18961 aaatttggaa acactttttt tgcccaatct tttgttggca cagagactat actccattta
    19021 catttatcag ttagagtgta agaacgccat tcttttaaag cttttccaga tgccttctga
    19081 aaatttgcaa ctctttcagg gtcaacatct tttaataaat ctggattttg ggcatagact
    19141 gataaaaatg ccccaccttc ttttgctata tctacatatt gttctgctgt ccatttgggg
    19201 aactcatgaa atgcttcatc tggagcatat aagtatttaa taagagtact ttcttcatca
    19261 gaccactcta tataaacatt tttagcacca catttatatg cttctttaag tgcatttctt
    19321 gcaaaatcag cacattctat tggtgacttt ataagtagaa tttgacctgg ctgtatgtta
    19381 actccaactt ttattgctaa ttcagcgtat ttttctaaat ttctatttaa attcatattt
    19441 atcctcctat atttttaata ttgaataact tataaagtat tataacatta cttatgtttt
    19501 taatattgaa caacttataa aatattataa ctttacttag at
//
LOCUS       DAYAPA010000072        19463 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.072, whole genome shotgun sequence.
ACCESSION   DAYAPA010000072 DAYAPA010000000
VERSION     DAYAPA010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19463
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.072"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(352..1551)
                     /gene="coaBC"
                     /locus_tag="ACGI59_002364"
     CDS             complement(352..1551)
                     /gene="coaBC"
                     /locus_tag="ACGI59_002364"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HFG9740149.1"
                     /translation="MLKDKTVVIGISGGIAAYKACDVVSKLKKLNANIHVIMTKSATE
                     FVKPLTFQSLSQNYVVEDMFEEPKTWEVEHISLAKKADLFLIVPASANVIGKIANGIA
                     DDMLTTTVMATKAQVLIAPAMNTNMYENPIVQKNIQTLKSFGYKFIEPESGRLACGDI
                     GTGKLANPDIIVEEVLTNLTKEQDLKGQSMIVTAGPTVESIDPVRYLTNRSSGKMGYS
                     IAREAIARGADVTLISGPTNLSIPQNLKKFVQIESAQDLYEAVVSNLSENKIVIQSAA
                     VADYKPKTYSDKKIKKKNDDLSIELSRNYDIAYEIGKIKEDKILIGFAAETNDLIEHA
                     KGKIKKKNLDFIVANDLTKEGAGFRADTNIVKIIDVEGNITEYPKMKKEEVANVILDK
                     VKALLEK"
     gene            complement(1556..1822)
                     /gene="rpoZ"
                     /locus_tag="ACGI59_002365"
     CDS             complement(1556..1822)
                     /gene="rpoZ"
                     /locus_tag="ACGI59_002365"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFG9740150.1"
                     /translation="MLKPSINEVLEKIDNRYYLVGTVSKRARKLIDGEEPYVSNKTKE
                     KPVCVATKEVASGKITYRLLTEEEIEIEEARHHAEQHQQISEEE"
     gene            complement(1825..2442)
                     /gene="gmk"
                     /locus_tag="ACGI59_002366"
     CDS             complement(1825..2442)
                     /gene="gmk"
                     /locus_tag="ACGI59_002366"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFG9740151.1"
                     /translation="MFVKKGLLLVVSGPSGAGKGTICKELLKENDTIKLSVSATTRKP
                     RTGEVDGVNYFFISKEKFEEMIEKGEFLEYAQIYDNFYGTPKAAIMECLEKGQDVLLE
                     IEMQGAKQIKEVCPEGVFIFVLPPSLKELKNRIVGRGTETEAEIEKRFSCAYEEIKMI
                     KDYDYFIFNEDVKTSAKEIEGIISSEKNKVSRYKNIIIEKFKEEL"
     gene            complement(2472..3353)
                     /locus_tag="ACGI59_002367"
     CDS             complement(2472..3353)
                     /locus_tag="ACGI59_002367"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFG9740152.1"
                     /translation="MAISMTGFGRGEYKDDNYYFLVECKTINHKYSDINIRLPRKISF
                     LEDKVRNLVKNYVKRGRVDLYIKFDLLGKEDVNLNFDEGLASQYIDILKEIKNKFDII
                     DDISVMNVAKFPDIVKIEEKEEDEDLLWSMLNQAVEDALIKLREMRSEEGKKLAEDIA
                     MRCDLLKNHIEEIEKYSSSVVEDYREKLNLRISELLDDPSIIDENRLAQEVAIYADKS
                     SITEEIVRFKSHIGQLKNTIFKDDSIGRKIDFLIQEMNRETNTIGSKSSDINITNLVV
                     EVKSELEKIREQIQNIE"
     gene            complement(3357..4187)
                     /gene="dapF"
                     /locus_tag="ACGI59_002368"
     CDS             complement(3357..4187)
                     /gene="dapF"
                     /locus_tag="ACGI59_002368"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFG9740153.1"
                     /translation="MKFWKLHGIGNDFIAIDGRFDQINPSDYTALAKKVCHRRFSVGA
                     DGLLVVKDSKVADVEMVYYNSDGSRAAMCGNGLRCFCKFVYDNCIVKKESFTVDTLDG
                     IKDIKVNLTNREINSIRVNMGKGSFIAKDVPVLTNKERFVQESIKVLDRELKVTSMLM
                     GVPHTVVIVDELDVDDVCKYGKEIENNKIFPEKTNVNFVKVEDKYNIHVYTWERGCGY
                     TLGCGTGMTASAIVCNLLNMVESSVNVTSQGGTVKIDVGDFSYMTGPAVKICEGILEV
                     "
     gene            complement(4474..5508)
                     /gene="ltaE"
                     /locus_tag="ACGI59_002369"
     CDS             complement(4474..5508)
                     /gene="ltaE"
                     /locus_tag="ACGI59_002369"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HFG9740154.1"
                     /translation="MRFIDLRSDTVTMPTDEMREAMAKAPVGDSIFRDDPTVNKLEEL
                     AAAKVGKEDAIFLPSGTFGNQLALFTHCLRGQEIIIGKGYHIVTHEVGAPAVIAGVQL
                     RTIDEDESGALNPELVEKAIRRDDIHEPQTGLICVENAYSGGTVVSLDNLREIKKIAE
                     KHNLPVHLDGARLFNAALTLGVEAKEIAKCCDSVMFCVSKGLAAPMGSILAGNKEFIA
                     KARRKQKVMGGGMRQVGIVAAAGIIAIEKMTLRLNEDHENAKYLACELNKIDGIEVNN
                     VNPDISMVFFKMSEDIIKEDVLIKEFFERNIKINKMENGEYRFVTHVDITKEDLDYVL
                     KTLKELIGVC"
     gene            5804..7579
                     /locus_tag="ACGI59_002370"
     CDS             5804..7579
                     /locus_tag="ACGI59_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT RNA binding domain-containing protein"
                     /protein_id="HFG9740155.1"
                     /translation="MALDGLVIHSIVDELSSKLTGGKIDKIHQPEDDEVIFNIRNNKE
                     NFRLVLSASASNPRVYLTSNYQKENPLKAPMFCMLLRKYIQGGNIVEISQIGFERIIK
                     ISVESLDELKEKTVKNIMIEIMGRHSNIIITHGEENKIIDSIKRVPFSISRVRQVLPG
                     HDYSLPPEQNKLNPLDDISKDLFIKNLEELEGPIFKSIYSKFLGISPIIAKEICYRAG
                     VNQNAIIKDISDEQFDSLHKVFCNLFNDINSNKYSPCIIIDKKVDKVVDFSCINLTLF
                     SDLSYINKDSMSRILEDFYRTKDIKDRINQRSSDLKKSISVKLDRLYNKLKKQEEELS
                     ESENADIYKIKGELITSYIYMVEKGMESIEVANFYDENCNDVTIELNKNLTPSENAQK
                     YFKKYNKMKHAKVEISHQISLNKEEIDYLENIILSIENCENLAELQDIKEELAKVGYI
                     KTQKKNSKKDTIPSTKPHEFVSSDGFKILVGKNNKQNDYLTLRLADNDDLWMHTKNIP
                     GSHVIIKCAGKEVPDNTVFEGAMLAAFFSKSKLSSQVPVDYTKRKNVKKPSGSKPGMV
                     IYETNSTIYVTPEEETVAKLKVKSE"
     gene            complement(7794..9446)
                     /gene="cplR"
                     /locus_tag="ACGI59_002371"
     CDS             complement(7794..9446)
                     /gene="cplR"
                     /locus_tag="ACGI59_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861613.1"
                     /note="Contributes to intrinsic pleuromutilin, lincosamide
                     and streptogramin A resistance; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein CplR"
                     /protein_id="HFG9740156.1"
                     /translation="MLLVKVENLKKYYADKLILDIDKLEILENDKIGLVGSNGQGKTT
                     LLKAILGEIEIDEGYTYLTESYSYISQSENNIETCSHSKEKSLLNAPDKFEEHLSGGE
                     KVKLKIADALSNKKNIIIADEPTSNLDKKSIGVLEDMFKRHEGALLLISHDRRFLDEL
                     CTTILELEDGKLKAYKGNYTDYLMQKDEEVKRADFEYQEYVKEKKRLEKALLYKKALS
                     DGIRKTPKRMGNSEARLHKMGGQTNKKKLDSNVKAIKSRIDKLEVKNKPKVSKEMNIK
                     IQDGMEIISKNLVEVKDMTLKLENKLLLDNVSFKIKRGKKIALLGDNGCGKSTLIKEI
                     LADKNDNIKINNKVKVGYFDQNQSLLDEEKSVLYNTKVNSSFDESFIRINLSLFGFKG
                     DDVYKKVKVLSGGEKVKIALCKIILEDNNFLVFDEPTNYLDIKSMEALEKALINTDKT
                     MLIVSHDRVFVSHICNYIIEIKDAKIREFDCNYDEYTISRNKKTPSRENQIKKENLLV
                     LENRLTTVISMLSIEKDNLKKELYESEYNELLKQINKLKNSF"
     gene            complement(9745..10956)
                     /locus_tag="ACGI59_002372"
     CDS             complement(9745..10956)
                     /locus_tag="ACGI59_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HFG9740157.1"
                     /translation="MKKTIMSLQHLLAMFGATVLVPILTGFNPSVAIFCAGVGTLIFH
                     FCTEGKVPAFLGSSFAFIPVILAAKEAYGGDLAYAQGGIIVAGLIYIIMSIIVKVVGV
                     NKIKLYFPAQVTGAMIVVIGLNLLPTAFSMASANFVIAFITLAIAILTNKFGRGFIKQ
                     LGILIAVFSGYIICLILRLVDITTITEASLFAIPNFTVPKFSLGAIVIISPVVLAVFM
                     EHIGDMTTNGAVVGKNFIENPGLNRTLLGDGFATIVAGCLGGPANTTYGENTAVLAIT
                     KNYDPSILRRTAIFAILLACVGKFGGFLQSIPGSVMGGISIMLFSMITYVGLKTIRDS
                     SCVESKINILIIAVILLIGLGTTYLSNKGISVGIPITSTVKITGLSLAAIVGIVLNRI
                     LNNQDFKVEEE"
     gene            complement(11240..11773)
                     /gene="pyrR"
                     /locus_tag="ACGI59_002373"
     CDS             complement(11240..11773)
                     /gene="pyrR"
                     /locus_tag="ACGI59_002373"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HFG9740158.1"
                     /translation="MVEKAQLMDEKAIARAITRISHEIIERNKGVENLVLVGIKTRGV
                     PIANRISKKIEQIEGTKVDTGDIDITLYRDDLEKIHVEPVVKGTYLDFNVNDKTVVLV
                     DDVLYTGRTVRASLDAIIDIGRPKSIQLAVLVDRGHRELPIRADYVGKNVPTSRHEII
                     SVSLLEIDGEDSVTIKE"
     gene            complement(12133..13080)
                     /locus_tag="ACGI59_002374"
     CDS             complement(12133..13080)
                     /locus_tag="ACGI59_002374"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFG9740159.1"
                     /translation="MFYFLKVKVGDIMDEVKEFLVLEEEEEVRLDIYLAEQLGDMSRS
                     YIQKIIKDGKVKVNNKVEKARYLVKEEDNIVIEIPEPKVLEVLPQDIPIDIVYEDDDV
                     LIINKPQDMVVHPAPGNYENTLVNGILYHCKDKLSSINGVIRPGIVHRIDKDTSGLLM
                     IAKNNYAHNFLAEQLKEHTITREYEFICYGVVKEDKITVDKPIGRNPKDRLKMAVVKD
                     GKNAITHFEVVERFDKFTHMRARLETGRTHQIRVHALSINHPLLGDYVYGPKETKFKL
                     KGQTLHAKKLGFIHPTTKEYIEFNSELPEYFKEVIKKIK"
     gene            complement(13049..13495)
                     /gene="lspA"
                     /locus_tag="ACGI59_002375"
     CDS             complement(13049..13495)
                     /gene="lspA"
                     /locus_tag="ACGI59_002375"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFG9740160.1"
                     /translation="MLYILIIILLIGLDQLSKIWVLNNLVDVSTIPIINNVFHLTYVE
                     NRGAAFGLLQNNQWIFIIVALLATVFGLYYLNTRKVHIFGRLGIILIISGALGNLVDR
                     VRLGFVVDYFDFRVIWEYVFNVADVFVVVGTVFLCIYVLFFESKSR"
     gene            complement(13805..14584)
                     /locus_tag="ACGI59_002376"
     CDS             complement(13805..14584)
                     /locus_tag="ACGI59_002376"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFG9740161.1"
                     /translation="MKKIICGICICLLSIVGVYTYNHNTHNQDSSKEAYSLNDNIKDD
                     YEDVIIKKGNEDEKILALTFDDGPDEDFTPQVLDILKKNDVKATFFVVGEKVEYNKEL
                     LKRQYDEGHEIGNHTFTHINVAKNSYSSVEKEITDTQNIIKSVIGVEPKIFRPPYRAM
                     SKSVCDIIVSKGMNIILWSNLDPRDWSNPGVDAIINTILTKVQNGNIILLHDYNTKRN
                     DKSQTIQALEVVIPKLKERGYKFVTVSELIQHLDKNKQVQK"
     gene            complement(14639..15121)
                     /locus_tag="ACGI59_002377"
     CDS             complement(14639..15121)
                     /locus_tag="ACGI59_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HFG9740162.1"
                     /translation="MDTKKYKDKLLKERENLTNLVEDMKDNTLFGDTTKHTSEKYSSG
                     ELSSYDNHIGDMGTDLYMQNMQNSLINHEEGRLYQIDLALSKIENGTYGICDLCHNQI
                     DLERLDILPDTSLCNDCAKKENNLLGDAMQNPDVEDSNFYSEDLTNLTDLNKNGILRD
                     "
     gene            complement(15387..16808)
                     /locus_tag="ACGI59_002378"
     CDS             complement(15387..16808)
                     /locus_tag="ACGI59_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HFG9740163.1"
                     /translation="MVSFLGSIVVLIVGYFVYGTFVEKVFGTNDKNQTPAIACRDGVD
                     YVPMKWKRIFLIQFLNIAGLGPIFGAIQGALFGPSAFLWIVFGTIFAGGVHDFASGYL
                     SMKNKGTSASELVGLYLGNGAKIAMRIFSVILLVLVGVVFVTGPAGLLKTLTGVNAQI
                     WVGVIILYYIMATVLPIDKLIGKIYPLFGAALLIMAVGVAGGLIIKGYNIPNINLQNM
                     NPNGTPLFPYLFITIACGAISGFHATQSPLMARCVEKESEARPVFYGSMVAEGIIALV
                     WAAAAMSYFHGIPQLNVIFNDGGAATVVNTVSVGLMGPIGGALAILGVVACPITSGDT
                     AFRSARLTIADAMNYNQDAVKNRFLIALPLFAVGVALTFIDFNIIWRYFSWANQTLAM
                     IMLWTGSAYLVKANKNHYITTLPALFMTVVTFSYIMQAKEGFRLPVNISNGIGVIVAI
                     ILGLLFFKKAKHIKEQNNHKLAS"
     gene            complement(16954..17133)
                     /locus_tag="ACGI59_002379"
                     /pseudo
     CDS             complement(16954..17133)
                     /locus_tag="ACGI59_002379"
                     /inference="COORDINATES: protein motif:HMM:NF016296.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
     gene            complement(17146..17724)
                     /locus_tag="ACGI59_002380"
     CDS             complement(17146..17724)
                     /locus_tag="ACGI59_002380"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFG9740164.1"
                     /translation="MRAIIVEDEFPARKELRYFIENKSGIEVVSEFTNGIEVLDFIQE
                     NKIDVIFLDINIPHLDGMLLAKTLNQFKSRPKIVFITAYESYAVDAFSLDVFDYILKP
                     YSEERIISMLNKLEKSEMSDIELFNVNSNLYKYKKEAVNQEIEEITHKISLWKGDKLV
                     VIDIDDIYYCEANERQTFIYTEKEKFILKEGI"
     gene            complement(17729..18931)
                     /locus_tag="ACGI59_002381"
     CDS             complement(17729..18931)
                     /locus_tag="ACGI59_002381"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFG9740165.1"
                     /translation="MKTSILIALIEKTSLIVVLFLLITKLKIFKQIFQKEEYSFNDLV
                     CIALVFTFLAIFGTYSGINYMGSIVNTRIISIVSGGILFGPMVGITAGVFSGIHRYFM
                     DIGGITSVPCLLSSILAGVLSGFLYKRIPKQHRVMYGILVGMISESFTILLIYLISYP
                     HSLAIQIIGGIYLPLIVGQIGIGFVISIVEGIEKDKKDIEARNKAEIKALQRQINPHF
                     LFNSLNTIASFIRFSPDKARELIINLSTYLRYNLEYSDNLIDINKEIEQVKSFVEIEK
                     ARFGELLTVSYDIEDVNIKIPSLIIQPLVENAIIHGILESGRAGVVKISIKKLPYSLE
                     NTVRISIEDNGIGISEEIINNVYQDNMPENKIGLYNVHLRLKLMYGRGLNIRRIDNGT
                     LIVFYVKE"
BASE COUNT         6410 a         3424 c         1968 g         7661 t
ORIGIN      
        1 acaggaattt gatatgtaaa taaattatct gtatatatag tattacttct aactattacc
       61 tttgcatatt ttttcattat aaaacctcta cttaaattta atatattgtt catattttaa
      121 tatactttac aagatattaa aatctcagaa tttataagca atttcaagtt attaatatta
      181 actttatttt aataattaac tattaattat attttatact ttatgacaaa ataaatgctg
      241 aaaactacaa tttaactgac atttgcgagt ttttactcgc taaaattaaa ttgcagcttt
      301 cagccacttt agctgtgata aaatacacaa atatcatatt taaaaaaggt cttatttctc
      361 aagcaacgct ttaactttat ctaaaataac attggctact tcttcttttt tcatcttagg
      421 atactcagta atatttccct ctacatctat tatttttaca atattagtat cagctctaaa
      481 accagcacct tctttagtta aatcatttgc cacaataaaa tctaaatttt tcttttttat
      541 ttttcctttg gcatgttcta taaggtcatt agtttcagct gcaaatccaa ttagtatctt
      601 atcttctttt atttttccta tttcataagc aatatcataa tttctactta gttctattga
      661 taaatcatca ttcttctttt taattttttt gtcagaataa gtttttggtt tataatctgc
      721 aactgctgca ctttgaatta ctattttatt ttcacttaaa tttgatacta ctgcttcata
      781 caaatcttgt gctgactcta tttgaacaaa ttttttaaga ttttgtggaa tagaaagatt
      841 tgtaggacct gatattaatg ttacatcagc acctctagct atagcttctc ttgctataga
      901 atatcccatt ttaccagaag acctattagt taaatatctt actgggtcta tactctctac
      961 agttggacca gctgtaacta tcatactttg accttttaaa tcttgctctt tagtaagatt
     1021 agtcaaaact tcctctacta ttatatctgg attagctagt ttacctgttc caatatctcc
     1081 acatgcaagc cttccacttt ccggttcaat aaatttatat ccaaagctct ttaaagtttg
     1141 tatatttttt tgaacaattg gattttcata catattagta ttcatagctg gtgctataag
     1201 aacctgtgct tttgttgcca taacagtagt tgtcaacata tcgtcagcta taccatttgc
     1261 aatcttgcct ataacatttg cacttgctgg cactattaag aataaatctg ctttttttgc
     1321 taatgatata tgttcaactt cccaagtttt tggttcttca aacatatctt ctactacata
     1381 attttgactc agcgattgaa atgtaagtgg tttaacaaat tctgttgctg attttgtcat
     1441 tataacatgg atatttgcat ttagtttttt taacttactt acaacatcac aagccttata
     1501 tgcagctata ccgccactta tgccaattac aacagtttta tcttttagca tgaccttact
     1561 cctcttctga aatttgttgg tgttgctcag catgatgtct agcttcttct atctctattt
     1621 cctcttctgt caataatcta tatgttattt ttccacttgc aacttcttta gttgctacac
     1681 atacaggctt ttccttagtt ttatttgaaa catatggttc ttctccatca attaattttc
     1741 ttgctctctt tgaaacagtc cctactaaat agtatctgtt gtctattttt tctagtacct
     1801 cattgataga tggttttaac ataattataa ttcctcctta aatttctcta ttataatatt
     1861 tttatatctg ctaactttat tcttttcaga tgatattata ccttctattt ctttagcaga
     1921 tgtttttaca tcttcattaa atataaaata atcataatcc ttaatcattt taatttcttc
     1981 atatgcacag ctaaatctct tctctatttc agcttctgtt tctgtacctc ttccaacaat
     2041 tctatttttt aattctttaa gtgatggtgg tagtacaaat ataaatactc cctctgggca
     2101 aacttctttt atttgttttg ctccttgcat ttcaatctcc agtaatacat cttgtccttt
     2161 ttctaaacat tccattattg ccgcttttgg agttccataa aaattatcat aaatttgagc
     2221 atactccaaa aactcacctt tttctatcat ctcttcaaac ttttccttac ttatgaagaa
     2281 ataatttact ccatcaactt ctccagttct gggttttctt gtagtagctg atacagaaag
     2341 ttttattgta tcattttctt taagtagttc tttgcaaata gtaccttttc ctgcacctga
     2401 tggtcctgat acaactagca ataatccttt tttcacaaac atttaagcat tccccttcta
     2461 atattgtatt cttactctat attttgtatt tgttctctta ttttttccaa ttcgcttttt
     2521 acttctacta ctaaattagt tatatttata tcagatgact tcgaacctat agtgttagtt
     2581 tctctattca tctcttgtat taagaaatct atttttctac ctatagagtc gtctttaaat
     2641 atagtatttt taagttgccc tatatgactt ttaaatctaa ctatttcttc tgttatactg
     2701 cttttatctg catatattgc aacttcttgt gctaatctat tctcatctat tatacttggg
     2761 tcatccagta attcacttat tctcaaattc aatttttctc tataatcttc tacaacacta
     2821 cttgaatatt tttcaatctc ttctatatga ttttttaata aatcacatct catggcaata
     2881 tcttcagcta attttttacc ttcttcactt ctcatttctc ttaatttgat taatgcatct
     2941 tcaactgctt gattcaacat tgaccacaat aaatcttcat cttcttcctt ttcttctatt
     3001 ttcacaatat caggaaactt tgcaacattc ataacgctaa tatcatctat aatatcaaat
     3061 ttatttttta tttcttttag tatgtctata tactgagatg ctaatccctc atcaaaattt
     3121 aagtttacat cttctttgcc taataagtca aactttatgt ataaatctac tcttcctctt
     3181 tttacataat tttttactaa atttctaact ttatcctcta aaaatgaaat ttttcttgga
     3241 agacgtatgt ttatatctga atacttatga tttatcgttt tacactctac taaaaagtaa
     3301 taattatcat ccttgtattc tcctcttcca aaaccagtca tacttatagc cattaattaa
     3361 acctccaaaa taccttcaca tatttttact gctggacctg tcatataaga gaaatctcct
     3421 acatctattt taactgttcc accttgagac gttacattta cactactttc taccatatta
     3481 agtaaattgc acactattgc tgatgcagtc ataccagtac cacaacctaa agtatatcca
     3541 caacctcttt cccaagtata aacatgtata ttatacttat cttctacctt tacaaaattt
     3601 acattcgttt tttcaggaaa tattttatta ttttcaatct cttttccata tttacaaaca
     3661 tcatctacat ctaattcatc tactataact actgtatgag gaacccccat caacatagaa
     3721 gtaactttaa gctccctatc taaaaccttt atactttctt gcacaaaacg ttctttattt
     3781 gtaagtacag gaacgtcctt agctataaaa cttccttttc ccatattgac tcttatagag
     3841 tttatttctc tattagtaag atttactttt atatctttta taccatctaa tgtatctact
     3901 gtaaaacttt cttttttaac tatacaatta tcataaacaa atttacaaaa acatctaagg
     3961 ccatttccac acatagctgc tcttgaacca tcagaattat aatataccat ttctacatct
     4021 gcaacttttg aatctttcac aactaacaat ccatccgctc ctacagaaaa tcttctatgg
     4081 catacttttt ttgctaaagc agtataatca gatggattta tctggtcgaa tcttccatct
     4141 attgctataa aatcatttcc tattccatgt agtttccaaa atttcataag tatcgctcct
     4201 tcgtaaatta acacttaaac tattataaca taaaaaaatt cccataaact tataatttat
     4261 gggaaaagtt ttgtaatgac aactataatt tgaggatgtg agggagagtt gccattacaa
     4321 ttagggtaat tatattatgt ttttaagcta gttctttcta tgtttatata ttaatatttc
     4381 tatatgaatt ttatgtatgt tttatttaaa ctgtctatga attttttaat taaacatctg
     4441 gtaactagct ataatccttc tagtagatat ctattaacat acacctatta attctttaag
     4501 tgtttttaaa acatagtcta aatcttcctt agtaatatct acatgagtta caaatctata
     4561 ttcaccattt tccattttat taatttttat atttctttca aaaaattctt ttattaaaac
     4621 atcctctttt ataatatctt cactcatctt aaaaaatacc atacttatat ctggattaac
     4681 attgtttact tcaatgccat caattttatt taattcacaa gctaaatatt ttgcattttc
     4741 atggtcttca ttcaatctta aagtcatttt ttcaatggca attataccag ctgctgcaac
     4801 tatacctact tgtctcattc ctccacccat aactttttgc tttcttctag cttttgctat
     4861 aaattcttta tttccagcaa gtatagaacc cattggtgca gctaaacctt ttgacacaca
     4921 gaacataaca ctatcacaac actttgctat ttctttagct tctacaccca aagttaatgc
     4981 tgcgttaaat aatcttgctc catctaaatg tactggtaag ttatgtttct cagctatttt
     5041 tttaatttct cttaaattat caagactcac aactgttcca cctgaatatg cattttctac
     5101 acatataagc cctgtctgtg gttcatgtat gtcatctctt ctaattgcct tttcaacaag
     5161 ttcaggattt agagctccac tttcgtcttc atctatagtt ctaagctgaa ctccagctat
     5221 tacagcaggt gccccaacct catgagtcac aatgtgataa cctttaccaa taattatttc
     5281 ttgccctctt aggcaatgag taaatagagc taattgattc ccaaaagttc cacttggaag
     5341 gaaaatagca tcttcttttc caactttagc tgctgctaat tcttcaagtt tatttacagt
     5401 agggtcatct ctaaagattg aatctccaac aggagccttt gccatagctt ctctcatttc
     5461 gtcagtaggc atagtaacag tatcacttct taaatcgata aatctcattt tttcaacctt
     5521 ctttcatatt tatatactat agaaccttaa aattgatttt ttctgtaaag ttcatatact
     5581 ttcatagctt atactctaca caataattat actatattta ctttatattt aatctacttt
     5641 aaattattaa ttttaaaaat aaagtttcct ctctaataaa tataaataat tatacaattt
     5701 aaatattaat ctctattgtt taaattcccc ctagtcacaa agcgtatatt atgtgtataa
     5761 ttaatttact gactcatttt ggtcaaagaa aggggtgcta taaatggcat tagatggatt
     5821 agttatacat tcaatagtcg atgaattatc atcaaaactt acaggtggaa aaatagataa
     5881 gatacatcaa cctgaagatg atgaggtaat ttttaatata agaaataata aagaaaattt
     5941 tagattggtt ttaagtgcaa gtgcctcaaa tcctagagtt tatcttacaa gtaattatca
     6001 aaaagaaaac cctctaaaag cgcctatgtt ttgtatgtta cttagaaaat acatacaagg
     6061 tggaaatata gtagaaattt ctcaaatagg ttttgaaaga ataataaaaa taagtgttga
     6121 atctttagat gaattaaaag aaaagacagt taaaaatatt atgattgaaa ttatgggaag
     6181 acatagcaac ataataataa ctcatggtga agaaaataaa attatagatt ctataaaaag
     6241 agtccctttt agcataagta gagttcgtca agttttacct ggtcatgatt attcattgcc
     6301 accagaacaa aacaaactca accctcttga tgatatatct aaggatttgt ttattaaaaa
     6361 cctagaggaa ttggaaggac ctatctttaa gtctatatat tctaaatttc taggtataag
     6421 ccctattata gctaaagaaa tttgctacag agcaggtgta aatcaaaatg ctatcataaa
     6481 ggatatatct gatgaacaat ttgattcttt acacaaagta ttttgtaatt tattcaatga
     6541 tataaattct aacaaatact ctccttgcat tattatagat aaaaaggtgg ataaggtagt
     6601 tgatttcagt tgtataaacc ttacattatt tagcgattta tcatatataa ataaggatag
     6661 tatgtctcgt attctggaag atttctatag aactaaggat attaaagata gaattaatca
     6721 acgttcatct gatttaaaga agagtatatc tgttaagctt gatagacttt ataataaact
     6781 taaaaaacaa gaagaagaat tatcagaatc cgaaaatgca gacatttata agataaaagg
     6841 agaattgatt acttcatata tttatatggt agaaaaaggt atggaaagta ttgaagttgc
     6901 taacttctat gatgaaaatt gtaatgatgt aacaatagaa ttaaataaaa acttgacacc
     6961 atctgaaaat gctcaaaaat actttaaaaa gtataacaaa atgaaacatg ctaaagtcga
     7021 gatatctcat caaatttcct taaataagga agaaatagat tacttagaaa acataatttt
     7081 aagtattgaa aactgtgaaa atttagctga attacaagat ataaaagaag aacttgctaa
     7141 ggttggatac atcaagactc aaaagaaaaa cagtaaaaag gatactatcc catctacaaa
     7201 acctcatgaa tttgtttctt ctgatggatt taaaatattg gttggtaaaa acaataagca
     7261 aaatgactat cttacactaa gacttgctga taatgatgac ttatggatgc atacaaagaa
     7321 tattcctggt tctcatgtaa taataaagtg tgctggtaag gaagtccctg ataatactgt
     7381 atttgaagga gctatgctag cagcattctt tagtaagtca aaactttctt ctcaggtgcc
     7441 tgtagactat actaagagaa aaaatgtgaa aaaacctagt ggttctaaac caggtatggt
     7501 tatttatgaa actaacagta ctatttatgt tactcctgaa gaagaaacag tagccaagct
     7561 taaggttaaa tctgaataaa ttctgcacta aatacaaaat aaatgttaga caactacaca
     7621 ttatcattct aaataaaaat agtggccttc ttataaagta caataataag gccactttct
     7681 aattagctta cttttaattt tctaacaaaa tacttttctg ttttaataac tatacttata
     7741 ttcccaacaa atattttcag tcttcttata tagatgtaat taaaaagtta acactaaaag
     7801 ctatttttta atttattgat ttgttttagt aattcattat attctgattc atacaattct
     7861 ttttttagat tatctttttc tatagataac atagatataa cagttgtaag tctattttct
     7921 aatactagaa gattttcttt tttgatttgg ttttctctac taggagtttt tttatttctg
     7981 ctaattgtat attcgtcata attacaatca aactccctta ttttagcatc ttttatctct
     8041 ataatatagt tacaaatatg tgatacaaat actctatcat gagacactat aagcatagtt
     8101 ttatcagtat ttatcagtgc cttttccagt gcttccatag attttatatc taagtaattt
     8161 gttggttcat caaatactaa aaaattatta tcttccaata ttattttaca tagtgctatt
     8221 tttacctttt ctccaccact aagtactttt acttttttgt agacatcatc acctttaaac
     8281 ccaaataggc ttaagtttat ccttataaaa gattcatcaa atgaactatt aactttagta
     8341 ttgtatagaa cactcttttc ctcatctaat aaactttgat tttggtcaaa ataacctact
     8401 tttaccttgt tatttatttt tatattgtca tttttatcag ccaatatttc ttttatcaga
     8461 gtactttttc cacacccatt atcacctaat aatgctatct ttttacctct ttttatctta
     8521 aaggaaacat tatctaacag gagcttattt tctaacttta gagtcatatc ttttacctct
     8581 actagatttt tactaattat ctccatacca tcttgaatct taatgttcat ttctttagaa
     8641 actttaggtt tattctttac ctcaagttta tcaattctgc tttttatagc cttcacattt
     8701 gagtccaact ttttcttatt agtctgacca cccattttgt gcaacctagc ttctgaatta
     8761 cccattcttt ttggtgtttt tcttatgcca tcacttaaag cttttttata taaaagagct
     8821 ttttcaagcc tttttttctc tttaacatat tcttgatact caaaatcagc tctttttact
     8881 tcttcatctt tttgcattaa ataatcagtg taattgcctt tgtaagcttt tagttttcca
     8941 tcttctagtt ctaatatagt tgtacataac tcatccagaa aacgtctatc atgagatatt
     9001 aataataatg ctccctcatg ccttttaaac atatcttcca aaaccccaat gcttttttta
     9061 tctaagtttg aagttggctc atcagctatt atgatatttt tcttattact cagcgcatct
     9121 gctattttta acttaacctt ttcccctcct gataagtgtt cttcaaattt atctggagca
     9181 tttaaaagac tcttctcctt actatgacta catgtttcaa tattattttc actctgactt
     9241 atataagaat agctttctgt aaggtaagta tatccttcat caatttctat ttctcccaat
     9301 attgctttta ataatgttgt ttttccttga ccatttgaac caactagacc tattttgtca
     9361 ttttctaata tttcaagttt atctatatcc aaaattaatt tatctgcata atatttcttt
     9421 aaattctcta cttttactaa caacataaaa aatccctcct taacacactt aaatataagg
     9481 agagattcta taaataggca taacaaataa acgtatagac tataatctac tctccttaat
     9541 ttaagcattg caaataaagc caccaaaata ggcttttcaa aaattacaat actttaaatt
     9601 ataaaaaata gattatattc tcattgtcct agttaccttg ccctttcttt catttatttt
     9661 taatcattat atcaacatta attacaaatg tctatagaaa attaactaat tttagttatt
     9721 caatatacaa cttattcttt aaatttattc ttcttcaact ttaaagtcct gattatttaa
     9781 aattctgttt aatacaattc caacaattgc agctaaacta agaccagtta tctttactgt
     9841 acttgttata gggattccaa cagaaatacc tttattagat aaatatgttg ttccaagacc
     9901 tattaataat ataactgcta tgattaatat atttatttta ctttctacac aagaactgtc
     9961 ccttatagtt tttaatccta cataagttat catcgaaaat agcattatac taattccacc
    10021 cataactgaa cctggtatac tttgaagaaa tccaccaaac ttgcctacac atgcaagcaa
    10081 tatagcaaat attgctgtac gtctaagtat tgaagggtca tagtttttag ttatagcaag
    10141 tactgctgta ttttctccat aagttgtatt tgctggtcct cctaaacacc ctgcaactat
    10201 agtcgcaaat ccatccccaa gtaatgttct atttaatcca ggattttcta taaagttttt
    10261 acctactaca gcaccattag tagtcatatc tcctatatgt tccataaata cagctaatac
    10321 aactggagat ataatcacta ttgctcctaa actgaactta ggaactgtaa agttaggtat
    10381 tgcaaataaa cttgcttctg taatagtagt tatatctact aatcttaata tcaaacagat
    10441 tatataccca gaaaaaactg ctattaatat tcctaattgt tttataaatc ctctaccaaa
    10501 cttattggtt aaaattgcta ttgctaaagt tataaatgct atcacaaaat tggctgaagc
    10561 catactaaaa gctgttggca ataaatttaa tcctataact actatcatag ctcctgtaac
    10621 ttgtgcagga aaatataatt taattttatt tacaccaact acttttacta taatcgacat
    10681 tataatataa attaatcctg ctactattat accaccttga gcatatgcta aatctccacc
    10741 atacgcctct ttagctgcta gtataactgg tataaacgca aaacttgaac ctaaaaaggc
    10801 aggtactttt ccttctgtac aaaaatgaaa tatcaatgta ccaacaccag cacagaaaat
    10861 tgcaactgac ggattaaaac ctgtcaaaat gggaactaat actgtcgctc caaacatggc
    10921 taatagatgt tgtaaagaca ttatcgtttt tttcataaaa acctccaaaa ttattatata
    10981 taaataaatc ctctgtttaa ttaatacctt ttaattaatt atcattccct agattctatt
    11041 ttttaacctc tatatactta ttatataatt tatcattatt ttacatttta cgacatcaaa
    11101 aactcaaata aaggagaata aaaactacat taagactcac atccataaaa ttataatatt
    11161 tattttgtgc aatctatgta agctttatcc tcctccctga ctattcaatg ctaagctttt
    11221 atataatact taaaaataat tattctttga ttgtaactga atcttctcca tcaatttcta
    11281 ataaactgac agatataatt tcatgtctag aagtaggtac attttttcct acataatctg
    11341 ctcttatagg caactctcta tgccctctgt ccactagcac tgctaattgt attgatttag
    11401 gtcttcctat atctataata gcatctagtg aagctctcac agttcttcca gtgtataaaa
    11461 catcatcaac taaaactact gtcttatcat ttacattaaa atctaaataa gttcctttaa
    11521 ctacaggttc aacatgaatt ttttccaaat catctctata caaagttata tcaatatctc
    11581 ctgtatctac ttttgttcct tctatttgtt ctattttctt agaaattcta tttgcaattg
    11641 gcacacctct agtctttatt ccaactaaaa ctagattttc tacaccttta tttctttcaa
    11701 ttatttcatg acttattcta gtaattgctc ttgctattgc cttttcatcc attaattgag
    11761 ctttttctac catatcataa ttattgtcaa agatataata tatctatttg acaaatctac
    11821 tcctttcttt taatttttta taaatttatt tttatttaaa atttcaattt aaacaacaaa
    11881 aaaacttctc atcacaagac aagaagctct tatataaaaa atatacagtg taatttagca
    11941 attgtcttac gatatctctg tatcgtttaa agataatttt ttaatctatc aactcaaatt
    12001 atatatttat atttcaaatt tttcaagctt ttttaaacaa aatactttaa tataagatat
    12061 tttatttaaa tttcactaaa tttatttact tatataaaaa caaaaaatga tttataagta
    12121 ttatacaaat ggttatttta tctttttaat aacttcctta aaatactcag gtaattctga
    12181 attaaattcg atatattcct tagtagtagg atgtataaat cccaacttct tagcgtgcaa
    12241 agtttgtcct tttaatttaa atttagtttc ttttggacca tatacataat cacctaaaag
    12301 tgggtgattt atagacaaag catgaactct tatttgatga gttctaccag tttcaagcct
    12361 agctctcata tgtgtaaact tatcaaatct ttctacaact tcaaagtgag tgattgcatt
    12421 ttttccatct tttactacag ccatttttaa tctatcctta ggatttctcc ctataggttt
    12481 atcaacagtt attttatctt ctttaaccac accatagcat ataaattcat actccctagt
    12541 tatagtatgt tcctttaact gttctgctag aaaattatgt gcataattgt tcttagctat
    12601 cataagaaga ccagaagtat ctttgtctat tctatgaact ataccaggtc taataactcc
    12661 atttattgat gacaacttat ctttacaatg atataaaatc ccatttacta acgtattttc
    12721 atagtttcca ggagcaggat ggacaaccat atcctgaggt ttatttatta ttaagacatc
    12781 atcatcttca tatacaatat ctattggaat atcttgaggt aaaacttcta atactttagg
    12841 ttctggtatc tctataacaa tattatcttc ctctttaacc agatatctag ctttttctac
    12901 tttattatta acctttactt ttccatcttt tataattttt tgtatataac ttcttgacat
    12961 atcacctaat tgttctgcta gataaatatc aagtctaact tcttcttcct cttcaagtac
    13021 taaaaactct tttacttcat ccattatatc acctactttt actttcaaaa aataaaacat
    13081 atatacataa aaacacagtt cctacaacta caaatacatc agctacattg aatacatatt
    13141 cccatataac tctaaagtcg aagtaatcta ccacaaaacc taatcgtact ctatcaacta
    13201 gatttcccaa tgcaccagat ataattaata taattcccaa ccttccaaat atatgtactt
    13261 tccttgtatt aagatagtat aatccaaata ccgttgcaag taatgcaaca attataaata
    13321 tccattgatt attttgtaat aatccaaatg ctgcacctct attttcgaca taagttaaat
    13381 gaaatacatt atttattatt ggtattgttg atacatccac caaattattc aatacccata
    13441 ttttagacag ttggtctaaa cctatgagta gaattattat taatatatat agcaatgatt
    13501 tttcccccta taaattattt aaaaaccaat tttatttgaa gcacactatt attattatac
    13561 aaaaaaaatg ctctaaagag caactttatt aaaattgtat tttcttaata aagctagaac
    13621 aaaatttaaa atagtactac tttttttata ttgtagattt ctacatattt aaaatcatat
    13681 cattatgtat aaaaaattta aaaataccat actaatcaat attttttgtg tattttatag
    13741 attaaagatt aatgatgtct gtaatttaaa cttacaatca ccactaatct tcaaacaaat
    13801 atttttactt ttgcacttgc ttatttttat ctaaatgttg tataagttca gatactgtta
    13861 cgaatttata tcctctctct tttaatttag gaattactac ttctaaagct tgtatagttt
    13921 gtgatttatc atttctttta gtgttataat catgtaaaag tattatatta ccattttgga
    13981 cctttgtaag gatagtattt ataattgcat ctactccagg gttagaccaa tctctagggt
    14041 ctaagtttga ccaaagaatt atattcattc ctttagaaac tataatatca cagacacttt
    14101 tactcatggc tctatatgga ggtctaaata tctttggttc tactcctatc acacttttta
    14161 taatattttg cgtatctgtt atttcttttt caacactact ataactattt tttgcaacat
    14221 ttatatgtgt aaatgtatga ttccctattt catgcccctc atcatattgt ctttttaata
    14281 attctttgtt atattctact ttttctccaa ctacaaaaaa agttgctttt acatcatttt
    14341 tctttaatat atcaagtact tgtggtgtaa aatcctcatc tggaccatca tcaaaagtca
    14401 atgctaaaat tttttcatcc tcatttcctt tttttattat tacatcttca taatcgtctt
    14461 ttatattgtc atttaaacta tatgcttctt ttgaactatc ttgattatgt gtattatgat
    14521 tatatgtata tacacctact atagataata aacatataca tataccacat attattttct
    14581 tcatgtcatt acctcttttc taattcattc tagcaatata ttttataatg tcagtttttt
    14641 aatctctcaa tattccattt ttatttaaat cagttaaatt tgttaggtct tctgaataaa
    14701 agttagaatc ttctacatca gggttttgca ttgcatcacc taataaatta ttttccttct
    14761 ttgcacaatc attacataaa cttgtatctg gcaatatatc caatctttct aaatctattt
    14821 gattatgaca taaatcacaa atcccatatg ttccattctc tattttagat aatgctaagt
    14881 ctatttgata caatcttccc tcttcatgat ttattagact gttttgcata ttttgcatat
    14941 ataaatctgt tcccatgtct cctatatgat tatcataact agataattct cctgatgaat
    15001 acttttcact tgtatgttta gttgtatctc caaataaagt attatctttc atatcctcta
    15061 ctaaattagt taaattttct ctttctttca ataatttatc tttatatttt ttagtatcca
    15121 tttttaacac ctctaatttt aatatttaca tcataaaaat agtatgtgga aaagtaaatt
    15181 aaacatacaa taaaaataag atttgattcg aaaaatggaa tgtctcaaaa tgaaattttt
    15241 agttcattta accctctttt ttatatgaaa tcaaatatat ttttatcctt tcagcaataa
    15301 aaaagagact tatctctatt ttgagatagt ctcttctttg aacataattt aaatacaatt
    15361 ttttaatttt tatagttatt attttactaa cttgccaatt tatgattatt ttgttcttta
    15421 atatgttttg cttttttaaa gaataaaaga ccaagaatta ttgctacaat aacacctatt
    15481 ccattagata tattaactgg tagtctaaat ccttcttttg cttgcattat ataactaaat
    15541 gttactacag tcataaaaag tgctggtaat gtagttatat aatggttttt atttgctttg
    15601 actaaatatg cagaaccagt ccacaacatt atcattgcaa gtgtttgatt tgcccaagag
    15661 aaatatctcc aaattatatt aaaatctata aatgtaagag caacaccaac tgcgaataat
    15721 ggtaaagcta ttaaaaatct atttttgact gcatcttgat tataattcat tgcatctgct
    15781 attgtcagcc tagcacttct aaatgctgta tctccagaag ttataggaca tgccacaact
    15841 ccaagtatag ctaaagctcc tccaataggc cccattaatc caacagatac tgtatttact
    15901 actgttgctg ctccaccatc attaaatatc acattaagtt gaggtatgcc atggaaatat
    15961 gacatggctg ctgctgccca aactaaagct attatacctt ctgcaaccat tgacccataa
    16021 aatactggtc ttgcctcact ctctttctca acacatctcg ccataagtgg tgattgagtt
    16081 gcatgaaaac ctgatattgc tccacatgct attgttataa ataagtatgg gaataaaggt
    16141 gttccatttg gattcatatt ttgtaaattt atatttggta tgttataccc ctttattatt
    16201 aatcctccag ctacacctac tgccataatt aataatgctg ctccaaataa aggatatatt
    16261 ttaccaatca atttatctat tggtaaaaca gtagccataa tataataaag tattatcact
    16321 ccaacccata tttgtgcgtt tacacctgtc aatgtcttta ataatcctgc tggacctgtt
    16381 acaaatacaa ctccaaccaa tactaaaaga ataacactaa atattctcat tgctattttt
    16441 gcaccatttc ctagataaag gcctaccaac tctgatgcag atgtaccttt gtttttcatt
    16501 gatagatatc ctgaagcaaa atcgtgtact ccaccagcaa agattgttcc aaacacaatc
    16561 cataaaaatg ctgatggtcc aaataatgct ccttgtatag ctccaaatat cggacctaac
    16621 cctgctatat ttagaaactg aattaaaaag attctcttcc atttcattgg cacataatct
    16681 actccatccc tacaagctat agctggagtt tgatttttgt cattggttcc aaaaaccttt
    16741 tctacaaaag taccatagac aaaataacct actataagta ctactattga acctaaaaag
    16801 cttaccataa attcatcccc ttagttttta tataattgct ttataaattt ttatgagttt
    16861 aagaaaacta tttccagttc aaatagtaat acttttttat gctaatataa atattaatta
    16921 cattaagtgc atatttaagt acatgaaatg actttatata tgcattaaca gcctaaattc
    16981 ttttactttg cttctactta cagttacttc ataatcacta ttttttaatt ttaatatgta
    17041 tgtattatta aaccatggta taatttcttt tactttggtt aagtttacga tataagacct
    17101 atgtgttcta aaaaatgttt tatcatttat aagattttcg acttcttata tgccttcttt
    17161 taatataaac ttttctttct cagtatatat aaatgtctgc ctttcattag cttcacagta
    17221 atatatatca tcaatatcta ttacaactaa tttatcacct ttccaaaggc taattttatg
    17281 tgttatttct tctatctcct gatttactgc ttccttctta tatttgtata aattactatt
    17341 tacattaaat agttcaatat cactcatctc acttttttct aatttattca gcatagaaat
    17401 aattctctct tcagaatatg gtttcaatat ataatcaaat acatccaaag aaaaagcatc
    17461 tactgcataa ctttcatatg ctgttataaa aacaatttta ggtcttgatt taaactgatt
    17521 taatgttttt gcaagtagca ttccatcaag atgaggtata tttatatcta aaaaaattac
    17581 atctatttta ttttcctgaa taaagtctaa cacctctata ccattggtaa attcacttac
    17641 tacctcgatt ccacttttat tttcaataaa atatctcaat tcctttcttg ctggaaattc
    17701 atcttccact attatagctc tcatacttct actcctttac ataaaatact atcaaagtcc
    17761 cattatctat tcttcttata ttaagccctc ttccatacat cagtttaagc cttaaatgta
    17821 cattataaag cccaatctta ttttctggca tattatcttg atatacatta tttataattt
    17881 cctcacttat acctatacca ttatcctcta ttgatattct cacagtattt tctaatgaat
    17941 acggtaactt ttttatggat attttcacta caccagctct accactttct aaaataccat
    18001 gtattattgc attttccact aatggttgta taatcaaact tggaatcttt atatttacat
    18061 cttctatatc ataagataca gttagtaatt ctccaaacct agctttttct atctctacaa
    18121 aagatttaac ttgttcaatc tctttattta tatcaattaa gttatcacta tattcaagat
    18181 tatatcttag ataagttgat aaatttatta ttaactctct agccttatct ggactaaatc
    18241 ttataaatga agctattgtg tttagggaat taaacaaaaa atgtggatta atttgtcttt
    18301 gaagtgcttt tatttctgct ttatttctcg cttctatatc ttttttatct ttctcaatcc
    18361 cctctactat agatataaca aatcctattc caatctgccc aactattaaa ggtaaatata
    18421 ttccaccgat tatttgaatt gctaaagaat gtggatatga tattaagtat ataagtaata
    18481 tagtaaaact ttcagaaatc atgcctacca atattccata cattactcta tgttgttttg
    18541 gtatcctctt atatagaaat cctgataata cacctgctag tatacttgat aaaagacatg
    18601 gaacagatgt tatcccccca atatccataa aatatctatg aatccctgaa aaaactcctg
    18661 ctgtaatacc taccatcgga ccaaataaaa tgccacctga tactatagat attattctag
    18721 tgtttactat tgaccccata taatttatac cactataagt tccaaatatg gctaaaaaag
    18781 taaaaactaa tgctatacag accaagtcat taaaactata ttcttccttt tgaaatatct
    18841 gcttaaatat ttttaacttt gttattagaa gaaacaatac tacaataagg cttgtctttt
    18901 ctattaatgc gattaaaatt gatgttttca taatccccct ttaaaaaata aatttttaca
    18961 cactctaaat ttattatggt tgttataaat tacatatttg aagtaatcct atcacaaaac
    19021 tttccacatt taatctttta attaaataag aaatagttgt acttctatta acaactaata
    19081 ctagctagca taagcttgta ataacttgta gttattatta actttacaat tctagtattt
    19141 aattttatac tttagaaatc tcttttctta gaaataatac tttcgaatat catataatac
    19201 aatattatgt agattcttac tcatacaaca tatttcttta aaaattttat actattcttt
    19261 gttcaaatat ctcatttgat ttaattgtgt taaacacaca ttatcatctc tctgttatat
    19321 atttattata tagccgtata tcgataaagt aaataaatcc catatttaaa ataatatgtt
    19381 tgacaataaa ttattataat gtatatttac cataataatt tgtcaacctt gaaagttact
    19441 tctataatta ttgttaaata att
//
LOCUS       DAYAPA010000073        19282 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.073, whole genome shotgun sequence.
ACCESSION   DAYAPA010000073 DAYAPA010000000
VERSION     DAYAPA010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19282
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.073"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            332..913
                     /locus_tag="ACGI59_002382"
     CDS             332..913
                     /locus_tag="ACGI59_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFG9740166.1"
                     /translation="MMGITLSKGQKVDLTKGNPGLKNILVGLGWDTNKYDGGFDFDLD
                     TAAFLTGASGSVTNDGDFVFYNNLKHTSGAVEHLGDNRTGEGDGDDEQIVVDLSKIPG
                     EISKISFTVTIHDATERRQNFGQVSNSYIRIVDKDTNEELIKYELGEDFSIETAIVVA
                     EIYKHNGEWKFNALGSGFEGGLAALCGNFGINL"
     gene            952..1530
                     /locus_tag="ACGI59_002383"
     CDS             952..1530
                     /locus_tag="ACGI59_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFG9740167.1"
                     /translation="MAIVLKKGQKIDLTKGNPGLKNIKLGLGWDTNSFDSGYDYDLDV
                     SVFMVGESQRVEKDEDFIFYNNLKHPSGAVEHLGDNRTGEGDGDDEEILVNLETMPKH
                     IQKIAVTVTIYEAAERRQNFGQVSNSYIRVLNSENDEEILRYDLGEEFSIETAITVCE
                     IYKYNGEWKFSAVGAGFEGGLEALCRNYGLNV"
     gene            1616..2245
                     /locus_tag="ACGI59_002384"
     CDS             1616..2245
                     /locus_tag="ACGI59_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFG9740168.1"
                     /translation="MSINLSKGDKIDLKKSNPGLSNILVGLGWDPVQQSGGGFFKSLF
                     GGGQADIDCDASVFMLNQEGKLSGIKDLIYFGNLKSACKSVLHTGDNLTGEGTGDDEQ
                     ILVNLDKVPSNIHKLLFVVNIYNCVDRKQHFGMIENAYIRVEDQGNKKEIAKYNLSDN
                     YSEKTTLIVGAIYRKDGSWQFKAIGEGTKDAGLKEVMQNLDRIECAYGI"
     gene            2235..4994
                     /locus_tag="ACGI59_002385"
     CDS             2235..4994
                     /locus_tag="ACGI59_002385"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005388 - P-type calcium transporter
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HFG9740169.1"
                     /translation="MEFNNDIKYYNVDKEEVTKDLSVNPEKGLSESEVKTRREKYGLN
                     EFTPKEEGSFWDDLKESLSEPMIVILIIAAVVSAVIGETHDAIGIVGAIAIGIAIGMI
                     TEGRSKKAAEALSKLTENIEVKVLRDGEVHQISKSELVPGDIVYIETGDMVPADGRLI
                     ESINLKIREDMLTGESDDVSKKCDVVVSMEEIESKGAVVVQEPIPAKQINMVFGGTLV
                     AYGRGALVVTSIGDNSEMGKIAQNLSETDEQTPLQIKLGNLGGLIAKVSSAVAGLLFI
                     FMVFQMISKNVLNVDMSGILPFLESIDPVKTAFTVCVALIVAAVPEGLPTMINMTLAI
                     TMQKMAKINALVTKKEACETIGSVSVICSDKTGTLTENRMTVEVAYVDGRYIESSEEE
                     INSYFEENCMINSTADVEHNDGDIKYLGSATECALLLYYKNVDYRQVRKNSNIVAQNP
                     FTSDSKRMTSVIGQDNHHVLLSKGAPEVLLELCSHIQRGKDIVPLTENIKHEILEEIK
                     KLQIKSMRTLGFAYKEISQAEEEAAVTAESDAMNVSAMENNLVFSGFVGIRDPLRKDV
                     IESVNTANKAGVSVKMLTGDNINTARAIGEELGLLKNNMRVVEASYIDTLDDEELKQE
                     IQSISIVARSKPDSKMRIVSALQKSGDVVAVTGDGINDAPALSKADVGIAMGISGTEV
                     SKNAADIILTDDSFSTIVKGIKWGRGIYDNFQRFVQFQLTVNVIAFLVAIISQVMGQE
                     MPFTTIQLLWVNIIMDGPPALALGLEPVRDHVLNRKPVDRKANIISKSMVYTIVLNAF
                     YITAILMLQSTFNFLGVDEAHKGTVMFALFAFSALFNAFNCREFNADSIFPNFTHNKL
                     ALQIIVLTGIAQVIFTQLFQDFFNSVAMDFMTWVKVLCVAASVIVVNEVVKCIIRLVK
                     LKKTA"
     gene            5081..6643
                     /locus_tag="ACGI59_002386"
     CDS             5081..6643
                     /locus_tag="ACGI59_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceG family protein"
                     /protein_id="HFG9740170.1"
                     /translation="MGDSKVFEKIFSSQSDRGNYTPSKGYLSYFISYIGLEDEVLYNL
                     EIFKTKQNIDSKKDIALFTDVIANPSDFDIINYFKSGLQKYRTSMEDVDINILGFEEI
                     DYKIKQAMDRVLKEEEKEFTNDRVKQNFIVKIMAWIKIYIGALDINKNEAPKVIFYGD
                     IKKHEVYLLLILYLAGFDVLYLNPNSKSNIDILKSERYNIEFEEANIIEEKISFEERV
                     ILGEKIDKSSVKKAFTVGAEASKRISEELLNDAGFIKPWQLQDRKIKNLLLSSTVDEI
                     SIYWNQPLKLRPGFKFNDAIVEAPNFLSKINGIYNDKNEYIKFLDLLRDSESSTFIEF
                     NGDVDRFSKAFTREAFSLSFLLDSKGAIDKNSVLNNKDYSISTLALNQQIMILEKVEE
                     LLEGSMFLNGLSGEDKIKGLFTVLHMDKKFVHMMNNFDYSLINPKLIIYMYKSIVFDK
                     EIVFLMLLLSKIGFDIIILCPGGENNIENVINNQLIDVHRLDKMVYDLKLNSLENDIP
                     LLKKIFGKRRRF"
     gene            6644..7780
                     /locus_tag="ACGI59_002387"
     CDS             6644..7780
                     /locus_tag="ACGI59_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HFG9740171.1"
                     /translation="MGININSFSKDKNSVEVTENLPTAVQEENFDIMEYTNNKKNELR
                     KSKEVEALTSLIEVENPDTILQFGRKASEGVARVSDSLLNTIKLNRNEENSKMLVHLT
                     KIMDKFDLDDFQETKEPNFVQKLFKKANNAIEMMFQKYETLGGEVEKIQIELEQYERD
                     IALSNKQIGAMLNENFEFYNELQKYIVAGEMAIEEMDNEILPFFKQKSETSGDQMDVV
                     NYQELLKVYDMLNQRVYDLRIAENIAIQTIPMLRGMQHNNYGLIRKINSAFVVTLPVF
                     KQCLSQAILLKKQELQAKSLKALDDKTNELLLRNAQNVSTQSAQIARMAGTSSVQIET
                     LEKMYNTIKSGIDETMRIEENNRALIKDNTKRLEELNTTIIYNK"
     gene            7844..8920
                     /locus_tag="ACGI59_002388"
     CDS             7844..8920
                     /locus_tag="ACGI59_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HFG9740172.1"
                     /translation="MKYFDYICKDDLERIFLKEPEDFSAKTEKDVLKYALGAFLYVPA
                     TQYNMIYKSIIGDVKGVRPLAICLEDAVGVNGELEAIENLRLILKNISNESITNKDGI
                     PLIFVRIKDVEQLLRIKEIIIKNSHFITGILIPKANSELIENCIEALDFMNLQDMYVI
                     PIVETKEFIYNEKKELSFTNLYNAILRHKSRILSIRVGITDILGMYGIRRDKNFSIYN
                     NLICSSFILDIITYLQRPELDIPISGGVSEFFDMTNKDIRNKYIEEILLDKYHGLIGK
                     TVIHPMQIQIVQALSTVSYEDFTDALDILDSTDSKYGVSKGVLGERMNETNPHFLWAK
                     KTLILSKIYGVLNKGVDYEELLKF"
     gene            8898..10136
                     /locus_tag="ACGI59_002389"
     CDS             8898..10136
                     /locus_tag="ACGI59_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HFG9740173.1"
                     /translation="MKNCLNFNCEIDIIDNPLNLELNDFLDVGIRNNSKRRFLFISKK
                     LGKHLACKPSEMDNLGKLMATIYNEKNKEESNGTVIAFAETGTAVGHSFFDYLCGDYE
                     FIHTTREQFDELENLEFLEEHSHATDQNLYFGMLNNFKFGDEIILVDDEITTANTCMN
                     IIRKIQSIYPKKKYTICSILNWVSDENLSKVTRLERELDCKIDFVYLFKGNFKFYIDE
                     DIISNYNDNYSITKLETISSDKVNLVVNHIYVDLEDYIGNKKYVKYTGRFGINRREQF
                     RLKEIVKRESKKITMASKESLKNEKEKILFLGTEEFMYIPMLFAKEKENQYDIYYHST
                     TRSPIINIDKNNYPIKSKFMLKSFYNIDIQNYIYNIDKHNFSKCYLFVELNKSENSYL
                     EFIDIIKKTSIKELTIVCCS"
     gene            10190..11278
                     /locus_tag="ACGI59_002390"
     CDS             10190..11278
                     /locus_tag="ACGI59_002390"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease StiP family protein"
                     /protein_id="HFG9740174.1"
                     /translation="MRDFSTYSKDDVIFLLKDISNLIEEEGNKERELKIQNGRHYSEM
                     IPIEYEVSEDYLNLYHEKLRENKDKLAFSIGVMCEKIIKKNGNDVVLVSLARAGTPIG
                     ILAKRYIRSKYNISLPHYTISIIRDKGIDMNAIEYIVKNHPSSKIQFLDGWTGKGTIS
                     KELEKACDELNVKFKKYFDSTLAVLADPAGYSGLYGIREDFLIPSACLNSTVSGLVSR
                     TVLREDLIGKNDFHGAKFYRHLKDKDESMNYIETIEECFKNQFKNISDEVENWESDII
                     TRDGYFDVLNIKEKYNITDINFIKPGVGETTRVLLRRVPYKILVKDLNDKSLDHIFIL
                     AKEKNVEVEQMDLKAYKCCGIIKNMKDI"
     gene            11306..12097
                     /locus_tag="ACGI59_002391"
     CDS             11306..12097
                     /locus_tag="ACGI59_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HFG9740175.1"
                     /translation="MIVFSDLDRSIIYSNKFLNADSKYTNIEIYREKEISYISLDTIN
                     LIKQIQYYGMFIPTTTRTVEQFKRIEFNKYGIYFPWSITSNGGVILKDNEILKSWSEK
                     IDKLKCDYEPIESMIHKFKDYLNVDGITNFKVAEDTFFYIVVDLSRFNLDSIKEYTNI
                     LESKNWKFYVSGRKIYFIPKEISKENAIKYLTKELGIEYFYAVGDSIMDYGMLNISNK
                     SYVLKHGDINKNEIENSFISSSFNGMSGTEEILSNILDENCLLNI"
     gene            complement(12212..12766)
                     /locus_tag="ACGI59_002392"
     CDS             complement(12212..12766)
                     /locus_tag="ACGI59_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFG9740176.1"
                     /translation="MNIDVGSKIRSLRKSKNISISTLAKNSDLSTGLISQIERNMVVP
                     SIVAMWKISKALDINIGYFFEEIGREDSDIVVKKNNRKKIVTNDSTKFYELLVPNLSG
                     KKIEFILVTLDGHTQKNSEFVTHEGEECGYIIKGKMKITLGNKEYLLEEGDSFYFNST
                     IPHVYENYDDEVCISVWAMTPPSF"
     gene            12962..14164
                     /locus_tag="ACGI59_002393"
     CDS             12962..14164
                     /locus_tag="ACGI59_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HFG9740177.1"
                     /translation="MKSFVSPDNIWVLWAVVTGWAAFSIYLEQKYNWASKVSGAIIAL
                     VGAMLLSNLNIIPVESVVYDQVWGYVVPLAIALLLYQCNIKKIWKESGRLLIIFLVGS
                     VGTVLGAMIGFLALKNVVPDLNIVAAMMTGSYIGGNVNFAAMSGAFDAPGELVSATVV
                     ADNLLMALYFFVLIAIPSIGFFRKHFKHPHVDEMESIGITEGETVAANYWGRKEISLK
                     DIAIAIGSAFIIVAVSVELSTWFKAIIPLSNPFLAMLNTLFGNQYLIITTLTMLFATF
                     MPNFFGDIKGAQELGTFLIYIFFVVVGVPASITLIIQKSPLLLLYCGIMVLINMLVTF
                     IVAKIFKFSLEEAILASNANIGGPTTAAAMAISKGWSKLVGPILIVGTFGYIVGNYFG
                     LLVGNILI"
     gene            14221..15201
                     /locus_tag="ACGI59_002394"
     CDS             14221..15201
                     /locus_tag="ACGI59_002394"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HFG9740178.1"
                     /translation="MIFDGHSDTWADVTIRRLNGEKDVFRRIHLDNFKRANIEGAIFV
                     IWIDPPYDKEPEKRVKQIISSIKDEIEDMKDVVNIVKKYSDIDKGISENKINILIGLE
                     GLSHIGRNIDLLDYFYKEVGARHASLTWNEQNELATGCLGDENRGLSEIGKKAVNKME
                     KLGMIVDVSHLNEKSFWDLMSVASKPVIASHSNARSLCDAKRNLTDNQLKEISNTGGL
                     VGINSFRGFTNSNPKKQDLIHLVDHIDYMVNLIGIEHVSFGFDFSDYLGKETIGSFTG
                     NASVPSVDGLESTHTAQNIVVELENRGYKKKDIELISSGNFYRLFKDILK"
     gene            15444..16397
                     /locus_tag="ACGI59_002395"
     CDS             15444..16397
                     /locus_tag="ACGI59_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFG9740179.1"
                     /translation="MKKRYLVIGIIILSYFSLFIGAEDINIMHIFAKNQHKLMIFIMS
                     RIPRLISILIAGVGMSIAGLIMQQISKNKFVSPTTGATIDAAQFGIVICMLLVPTASI
                     FTKTIIAFVFSLVGTFTFMKIIGKLQFKNIIFVPLVGIMFGNIIGSMTDFIAYKYDLS
                     QNVSSWMQGDFSMILKGNYEILYITIPLIILAYIYANKFTVVGMGMDFATNLGLSYKR
                     IVNIGLIIVALVTVCVVVTAGNIPFIGLIVPNIVSLYMGDNIRASIWYTGLLGAIFVL
                     ICDIFGRIIIYPYEISIGLTVGVIGSILFLYLILRRNVNEA"
     gene            16387..17343
                     /locus_tag="ACGI59_002396"
     CDS             16387..17343
                     /locus_tag="ACGI59_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFG9740180.1"
                     /translation="MKLSLNQKIYFVSILLFISMALFIIYGIDMNHLEYALSQRIPKI
                     FAMVLGGGCIAFTTVVFQTITNNQILTPSVLGLDSLYVMIQTIIVFIFGSSSSLIINE
                     NYNFIINVAMMIGASLLLYKTLFEKNKNNIFFLILVGMIFGTLFKSATTFIQVMIDPN
                     EFLALQTSIMASLNNINTNVLLIAFIIIIAIIPFIYDEIKYLDVLSLGKEQAINLGVD
                     FDKAVKKMIILIAILVSISTALIGPMTFLGLLLANITREIFKTYKHTYLISGSMLIGM
                     ITLIIGQFFIQHVFKFDTTLSVVINFIGGIYFIQLLLKGANR"
     gene            17340..18095
                     /locus_tag="ACGI59_002397"
     CDS             17340..18095
                     /locus_tag="ACGI59_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9740181.1"
                     /translation="MIEIKNIFKRYKNKNVVDDVSFSIEKGKITSFIGPNGAGKSTVL
                     SIVTRLIGGDGGEVIIEGKSLTNYSNKELAKKIAILKQSNNITLKLTIRELVGFGRFP
                     YSEGNLTKEDENYIDEAIEYMKLTDIQHKYLDELSGGQRQRAYIAMVIAQDTEYILLD
                     EPLNNLDMNHSVQMMKVLRGLCDELDKTIVLVMHDINFASCYSDNIVALKNGKVEKVG
                     RTDEIVNEKVLEDIYEMNFNIKNINGNRICIYF"
     gene            18187..19152
                     /locus_tag="ACGI59_002398"
     CDS             18187..19152
                     /locus_tag="ACGI59_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFG9740182.1"
                     /translation="MNKKAAIVAAVAIIGLVTVFALGGSKKNESKTSENSNNTMKITH
                     NLGETDVKLNPKKVVVFDYSALDTMDALGVAETLVGLPKASLPASLEKYKDDKYADLG
                     GLKEPDLEGIKSANPDLIIINGRQEDFYEQLSKIAPTISTSKDDKKYLESVKNNIDKI
                     AKIFGVEEKANQEFSKIEKKIETLNKKVTDKNLNALTIMVNEGNLSVFGEESRFSILY
                     NSFGFENKDKNIKESSHGQNITFEYIAKQNPEVMFVIDRGIATGSDVKESSTAKSVLN
                     NDIIKSMDAYKNDNIIYLDSPTWYVNDGGLTSLNKMIDDASKAVN"
BASE COUNT         7619 a         1962 c         3453 g         6248 t
ORIGIN      
        1 ggtagtagtg gcgaagacac cgtccgaagt agcttggctt tgtaaccacc tctgttgtac
       61 tgtttaagta taaataagtg tgttagccac caagaatccc cttgctttag ctatggggag
      121 tgtgtcaaat attaaacata gcttttatac atttaataag gtatgtaaaa tctatgtaaa
      181 aagtaagaaa tataaacatt tttttaatta tattttaaaa atatcaagca agttttcttc
      241 acaaatgaat aaaaaatgtt ataatatgat taaagaaaaa gataaataat tagcaagatt
      301 tgtttagcta aaattaacaa gggagcgata aattatgggt ataacattat caaaaggcca
      361 aaaggttgat ttaactaaag gaaatccagg tcttaaaaat attttagtag gtttaggatg
      421 ggatactaac aagtatgatg gtggatttga ctttgattta gatacagcag catttttaac
      481 aggagcaagt ggaagtgtta ctaatgatgg agattttgta ttttacaaca atttaaaaca
      541 tacatctgga gcagttgagc atctaggaga taacagaact ggcgaaggtg atggagatga
      601 tgaacaaata gttgttgatt tatctaagat tcctggagaa ataagcaaga tttcttttac
      661 tgttacaatt catgatgcaa cagaaagaag acaaaatttt ggacaagtaa gtaattctta
      721 tataagaata gtagataaag acacaaatga agagttaata aagtatgaat taggagaaga
      781 ttttagtata gaaacagcta tagtagtagc tgaaatatat aagcacaatg gagaatggaa
      841 gtttaatgca ctaggttctg gctttgaagg tggtcttgct gcattatgtg gaaactttgg
      901 aataaactta taatttcaac tataacaata aactaaaggg ggatattttc catggctata
      961 gtattaaaaa aaggacaaaa aatcgattta actaagggaa atccaggtct taaaaatata
     1021 aaacttggac taggatggga tacaaattca tttgatagtg gctatgatta tgatttagat
     1081 gtaagtgttt tcatggttgg agaatctcaa agagttgaaa aagatgaaga ttttatattc
     1141 tacaataact taaaacatcc atctggagca gttgaacact taggtgataa tagaactggt
     1201 gaaggtgatg gagatgatga ggaaatatta gtgaatttag agacaatgcc aaagcatata
     1261 caaaagatag ctgtaacagt tacaatatat gaggctgctg aaagaagaca aaattttgga
     1321 caagtaagta actcatatat aagagtatta aattctgaaa atgatgaaga aatattaaga
     1381 tatgacttag gtgaagaatt tagtatagaa actgcaatta cagtatgtga gatatataag
     1441 tataatggag aatggaagtt tagtgcagtt ggagcaggat ttgaaggtgg cttagaagca
     1501 ctttgtagaa actatggctt aaatgtttaa tttaagcaat ataatatatt aaatataaat
     1561 aacctataca aataatgtat aggttatatt atataaaatt ttgtggaggt ttagtatgtc
     1621 gattaattta tcaaaaggtg ataaaataga tttaaaaaaa tctaacccag gactgagtaa
     1681 catacttgta ggtctaggat gggaccctgt gcaacaatct ggtggaggtt tttttaaatc
     1741 gttatttggt ggaggacaag ctgatataga ttgtgatgca tctgtattta tgcttaatca
     1801 agaaggtaaa ttaagtggaa taaaagattt aatttatttt ggaaatctaa aaagtgcctg
     1861 taaatcagtt ttgcatacag gagacaatct aactggtgag ggtacaggtg atgatgaaca
     1921 aatcctagtt aatttggata aagttccatc aaatattcat aaattattgt ttgtagtaaa
     1981 tatatacaat tgtgtagata gaaaacaaca ctttggaatg atagaaaatg cctatataag
     2041 agttgaagac caaggtaata agaaagagat agcaaaatac aatctatctg ataattattc
     2101 tgaaaaaact actcttatag taggagctat atatagaaaa gatggaagtt ggcaatttaa
     2161 agcaattggt gaaggaacaa aagatgcggg tcttaaagaa gtaatgcaaa atttagacag
     2221 gattgagtgt gcatatggaa tttaataatg atatcaaata ttataatgta gataaagaag
     2281 aagttacaaa agacttatct gttaatcctg aaaaaggttt atcagaatca gaagtcaaga
     2341 ctagaagaga aaaatatggt ttgaatgaat ttactccaaa agaagagggt agtttttggg
     2401 atgatttaaa ggaaagttta agtgaaccaa tgatagttat cttgattatt gcagcagtag
     2461 taagtgcagt aattggggaa actcatgatg caatagggat tgtaggtgct atagcaatag
     2521 gtatagcaat aggtatgatt acagaaggtc gttctaagaa agcagcagag gctctttcta
     2581 agttgactga aaacatagaa gtaaaagtat tgagagatgg ggaagtacat caaatttcta
     2641 agagtgaatt ggtaccaggt gatatagtat atattgaaac tggtgacatg gtacctgctg
     2701 atggtagact tatagaaagt ataaacctta aaattagaga agatatgtta acaggagaat
     2761 ctgatgacgt atctaaaaag tgtgatgtag tagtttctat ggaagagata gaatctaaag
     2821 gagcagtagt agtacaggag ccaatacctg caaaacagat aaatatggtg tttggtggca
     2881 cacttgtagc ttatggtaga ggagctctag ttgtaacttc aattggagat aatagtgaaa
     2941 tgggtaaaat tgctcaaaac ttatcagaaa cagatgagca aactccactt cagataaaac
     3001 ttggaaattt aggtggtttg atagccaaag tttctagtgc agtagcagga ttactattta
     3061 tatttatggt tttccaaatg ataagtaaga atgtattaaa tgtagatatg tctggaatat
     3121 taccgttttt agaatcaata gacccagtta aaacagcatt tacagtatgt gttgcgttga
     3181 tagttgcagc agtgccagaa ggtcttccaa ctatgataaa tatgacactt gcaataacaa
     3241 tgcaaaaaat ggctaaaata aatgcattgg ttactaaaaa agaagcttgt gaaacgatag
     3301 gttctgtttc tgtaatatgt tcagataaaa ctggtacatt aactgaaaat agaatgactg
     3361 tagaagttgc atatgttgat ggcagatata ttgaaagttc agaagaggaa ataaatagtt
     3421 attttgaaga aaactgtatg ataaactcta cagctgatgt agagcacaat gatggtgata
     3481 ttaagtattt aggaagtgct actgaatgtg cattgctttt atattacaaa aacgtagatt
     3541 atagacaagt tagaaaaaat tctaatatag tagctcaaaa tccttttact tcagattcaa
     3601 agagaatgac ttctgttata ggtcaagata atcatcatgt actattatca aaaggagcac
     3661 cagaagtctt acttgaatta tgttcacata ttcaaagagg aaaagacata gttccattaa
     3721 ctgaaaatat aaaacatgag attttagaag aaataaagaa attgcaaata aaatcaatga
     3781 gaactttagg gtttgcatat aaagaaatct cacaagctga agaagaagca gcagtaactg
     3841 ctgaaagtga tgctatgaat gtaagtgcaa tggaaaacaa tttagtattt agtggttttg
     3901 ttggtataag agacccactt agaaaagatg ttatagagtc tgtaaataca gcaaataaag
     3961 ctggtgtttc tgtaaaaatg cttacaggag ataatataaa tacggcaaga gctataggtg
     4021 aagagttagg cctattaaaa aataatatga gagtggttga agcttcatat atagatactt
     4081 tagatgatga agaattaaaa caagagatac aaagtatttc aatcgtggca agaagtaaac
     4141 cagatagtaa aatgagaata gtttcagctc tgcaaaagag cggagatgtt gttgcagtaa
     4201 caggtgatgg tataaatgat gctccagcac tttcaaaagc agatgttggt atagcaatgg
     4261 gtatatctgg tacagaggta tctaaaaacg cagcagacat aattttaact gatgatagtt
     4321 ttagtaccat tgtaaaaggt attaaatggg gtagaggaat ttatgataac ttccaaagat
     4381 tcgttcaatt ccaattaact gtaaatgtaa ttgcattttt agtggctata atatctcaag
     4441 ttatgggaca agagatgcca tttactacaa tacaattatt atgggtaaac atcattatgg
     4501 atggaccacc agcactagca ttaggtttag agccagttag ggaccatgtg ttaaatagaa
     4561 aacctgttga tagaaaagcc aatataattt ctaagtcaat ggtgtataca atagtattaa
     4621 atgcatttta tataacagca atactaatgt tacaatctac atttaatttc ttaggagttg
     4681 atgaagcgca taagggaaca gtaatgtttg cgttatttgc ttttagtgca ctgtttaatg
     4741 catttaactg tagagagttt aatgcagata gtatcttccc taattttaca cataacaaat
     4801 tagcactaca aataatagtg ttgacaggaa ttgcacaagt aatatttact cagctattcc
     4861 aagatttctt taactctgta gcaatggatt ttatgacatg ggtaaaagtt ttatgtgtag
     4921 cagcaagtgt aattgtggtt aatgaagttg taaaatgtat aataagactg gtaaaactaa
     4981 aaaagactgc ttaaaacaaa ttttttagaa gaaaacagac tgactcaaaa tatgaatttt
     5041 gggtcagttc tgaataaaca tttaaattgg gaggatagtt atgggtgact caaaggtatt
     5101 tgaaaaaata ttctccagcc aatcagatag aggaaattac actccttcaa agggatatct
     5161 aagttatttt atttcgtata ttggtttaga agatgaagtt ttgtacaatc tcgagatatt
     5221 taagacaaaa caaaatattg atagcaaaaa agatattgct ttatttactg atgttatagc
     5281 aaatcctagt gattttgata ttataaatta ttttaaaagt ggacttcaaa aatatagaac
     5341 tagcatggaa gatgtagata taaatatttt gggatttgaa gagattgatt ataaaataaa
     5401 gcaagcaatg gatagagtgt taaaagaaga ggaaaaagaa tttactaatg atagagttaa
     5461 acaaaatttt atagtgaaaa taatggcatg gataaaaatt tatatcggtg ctttagatat
     5521 aaataaaaat gaagctccaa aagtaatatt ttatggagat ataaaaaaac atgaagttta
     5581 tttactctta atattatact tggcaggttt tgatgtttta tatcttaatc caaattcaaa
     5641 gagtaatata gatattttaa aatcagaaag atacaatata gagtttgaag aagccaacat
     5701 aatagaagaa aaaatttctt ttgaagaaag ggttatttta ggggaaaaaa tagataaaag
     5761 ttctgtaaaa aaagctttta ctgttggggc agaagcatca aagcgtataa gtgaggaatt
     5821 gttaaatgat gcaggattta taaaaccttg gcaactccaa gatagaaaaa taaaaaatct
     5881 acttctaagt tcaactgttg atgaaatatc aatatactgg aatcaaccac ttaaattaag
     5941 accaggattt aagtttaatg atgctattgt agaagctcca aattttcttt ctaagattaa
     6001 tggaatatat aacgataaga atgaatatat taagttttta gatttattaa gagattcgga
     6061 aagttcaaca tttattgaat ttaatggaga tgtagataga ttttcaaaag cttttacaag
     6121 agaagctttt agtttatcct ttttattgga ttcaaaaggt gcaatagata aaaactctgt
     6181 tttaaataat aaagattatt ccatatcaac actagcattg aatcaacaaa ttatgatttt
     6241 agagaaagtg gaagagttgt tagaaggaag tatgtttttg aatggtttaa gtggagaaga
     6301 caagataaaa ggtctattta cagtacttca catggataag aaatttgttc atatgatgaa
     6361 caatttcgat tactcactta taaacccaaa attgattata tacatgtaca agagtattgt
     6421 atttgataaa gaaatagttt ttcttatgct tttacttagt aagattggat ttgatattat
     6481 catattatgt ccaggaggag aaaacaatat agagaatgtt ataaataacc aattgattga
     6541 tgtacacaga ttggataaaa tggtatatga ccttaaatta aattcgcttg aaaatgatat
     6601 acctttactt aaaaaaattt ttggaaagag gagacgattt tagatgggga taaatataaa
     6661 tagtttttca aaagataaga actctgtaga ggttactgaa aatttgccta cagcagttca
     6721 agaagaaaac ttcgatataa tggaatatac aaataataaa aaaaatgaac tgagaaagtc
     6781 taaggaagtt gaggcattga catctcttat agaagtagaa aatccagata cgattttaca
     6841 gtttggtagg aaagcatctg aaggagtagc aagagtttct gatagccttt taaatacaat
     6901 aaaattaaat agaaatgaag aaaattctaa aatgttggtt catttaacta aaataatgga
     6961 caagtttgat ttagacgatt ttcaagaaac taaggaacca aatttcgttc aaaaactttt
     7021 caaaaaagct aataatgcca ttgaaatgat gtttcaaaag tatgaaacat tgggaggaga
     7081 agttgagaaa attcaaattg agcttgaaca atacgaaaga gatatagcac tttctaataa
     7141 gcaaataggt gctatgttaa atgaaaactt tgagttttac aatgaacttc aaaaatatat
     7201 tgttgctgga gaaatggcaa tagaagaaat ggataatgaa atactacctt tctttaaaca
     7261 aaaatcagag actagtggag accaaatgga tgttgtcaat tatcaagaac tattaaaagt
     7321 ttatgatatg ttaaatcaaa gagtctatga tttaaggata gcagaaaata tagctatcca
     7381 aacaatacct atgcttagag gtatgcaaca taataattat ggtttaatta gaaaaataaa
     7441 tagtgcattt gtcgtaactt taccagtatt taaacaatgt ttatctcaag ctatattact
     7501 taaaaagcaa gaattacaag caaaatctct aaaagcactt gatgacaaaa ctaatgaatt
     7561 attgcttaga aatgcacaaa atgtaagtac tcaaagtgca caaatagcta gaatggctgg
     7621 aacaagttct gttcaaatag aaacattgga aaaaatgtac aatactatca agtctggtat
     7681 agatgaaact atgagaatag aagaaaacaa tagagcttta attaaggata atacaaagag
     7741 attggaagaa ttaaatacga ctataattta taataagtag tattatatta agggctaatt
     7801 tgtataaagt tggccctttc tatgtatatg aggaggctag acattgaaat atttcgatta
     7861 tatctgcaaa gatgatttgg agagaatttt tttgaaggag ccagaagatt ttagtgctaa
     7921 gactgaaaaa gatgtgttga aatatgcact gggcgctttt ttatacgttc cagctactca
     7981 atataatatg atttataaga gtattatagg ggatgttaag ggagttagac cattagctat
     8041 ttgtttagaa gatgctgttg gagtaaatgg agagttggag gctatagaaa atcttagatt
     8101 aattttaaaa aatattagca atgaaagtat aactaataaa gatggaatac cactaatttt
     8161 tgtaagaata aaagatgtgg agcaactgtt gagaattaaa gagattataa ttaaaaatag
     8221 tcattttata acaggaatat taattcctaa agcaaattcg gagttaattg aaaattgtat
     8281 agaagcttta gattttatga atcttcaaga tatgtatgtt attccgatag tagagacaaa
     8341 agaatttata tataatgaaa aaaaagaact tagttttact aatttgtaca atgctatttt
     8401 aagacataag tcaagaatat taagcattag agtaggcata actgatatat tagggatgta
     8461 tggtataagg agagataaaa acttttctat atataataat ttgatttgtt cttcatttat
     8521 attagatata atcacatatc ttcaaagacc agagttagat atacctataa gtggtggagt
     8581 atcagaattt tttgatatga cgaataagga tataagaaat aagtatatag aagaaatatt
     8641 attggataaa tatcatggat taattggaaa gaccgtaatt catccaatgc aaattcaaat
     8701 tgtacaagcg ctatctacag tatcatatga agattttact gatgctttgg atatactgga
     8761 tagtacagac agtaagtatg gtgtgagcaa gggtgtatta ggagagagaa tgaatgaaac
     8821 aaatccacat tttttatggg ctaaaaaaac attgattcta tctaaaatat atggtgtatt
     8881 aaataaagga gtagactatg aagaattgct taaattttaa ttgtgagatt gacattattg
     8941 ataatccatt gaatttagaa ctaaatgatt ttttagacgt aggaataaga aataatagca
     9001 aaagacgttt tttgtttatc tcaaagaaac tcggtaaaca tttagcatgt aagccaagtg
     9061 agatggataa tttgggaaaa ttaatggcta caatttataa tgaaaaaaat aaggaagaat
     9121 caaatggtac tgttatagct tttgctgaaa ctggtacagc tgttgggcat agtttttttg
     9181 attacttatg tggagattat gaatttattc atactactag agagcagttt gacgagttag
     9241 aaaatctgga atttttagag gaacattccc atgcaacaga ccagaattta tactttggta
     9301 tgcttaacaa ctttaaattt ggagatgaga taatattggt tgatgatgag attaccacag
     9361 ccaatacatg catgaatata attaggaaaa ttcaaagcat ttatcctaaa aagaaataca
     9421 ctatatgctc aatactcaat tgggtaagtg atgaaaattt aagtaaagtt actcgtttag
     9481 aaagagagtt agattgtaag atagattttg tatacttatt taaaggaaac tttaaatttt
     9541 atatagatga agatattatt tcaaattaca acgataatta tagtattact aaattagaga
     9601 ctatttcaag tgataaagta aatttagtag taaatcatat ttatgttgat ttagaagatt
     9661 atattggaaa taagaaatat gtaaaatata caggaagatt tggaattaat agaagagaac
     9721 aatttagatt aaaagaaatc gttaagagag aaagtaaaaa aataactatg gccagtaaag
     9781 aatctttaaa aaatgaaaaa gaaaagattc tatttttagg tactgaagaa tttatgtata
     9841 tacctatgct gtttgcaaaa gaaaaagaaa atcaatatga tatatactat cattcaacaa
     9901 caagaagtcc aattattaat attgataaga ataattatcc tattaaaagt aaatttatgc
     9961 taaagagttt ttataatata gatattcaaa attatattta caatatagat aaacataatt
    10021 tttctaagtg ttatttattt gtagagctga ataaaagtga aaattcttat ctagaattta
    10081 ttgatataat taaaaaaaca agtataaaag aattaactat agtatgttgt agctaaagtg
    10141 ttttaacagg ataaaatata tcacgatttt aagataggag ttgagtcata tgagagattt
    10201 tagcacgtat tctaaagatg atgttatttt tttattaaaa gatatatcaa atttaattga
    10261 agaagaagga aataaagaaa gagaattaaa aatacaaaat ggtagacatt attcagaaat
    10321 gataccaata gagtatgaag ttagtgaaga ttatctgaat ttatatcatg agaaactaag
    10381 agaaaataaa gataaacttg cattttctat aggagttatg tgtgaaaaaa taattaaaaa
    10441 aaatggaaat gatgtagtac tagtttcact tgctagagca ggaacaccta ttggtatttt
    10501 ggcaaaaaga tatataagaa gcaagtataa tataagttta cctcactata caatctctat
    10561 aataagagat aagggaatag atatgaatgc aattgaatat atagtaaaga atcatccgag
    10621 ctcaaaaatt caatttttag atggatggac tggtaaggga actatatcaa aagaacttga
    10681 aaaagcatgt gatgaactta atgttaaatt taaaaaatat tttgactcaa cacttgctgt
    10741 attagctgac cctgctggat attcaggact ttatggaatt agagaagatt tcttaatacc
    10801 aagtgcatgt ttaaattcaa cagtttctgg tttagtaagt agaactgtac taagagagga
    10861 tttaattggt aaaaatgact tccatggagc taagttttat agacatttaa aagataaaga
    10921 tgaatctatg aactatatcg aaactataga agaatgtttt aaaaatcaat ttaagaatat
    10981 atctgatgaa gttgaaaact gggaaagtga cattataaca agagatggat actttgatgt
    11041 tttaaatata aaagaaaagt ataacataac agatattaac tttatcaaac caggtgtagg
    11101 ggaaacgaca agggttttac ttagaagagt tccgtataag attcttgtta aagacttaaa
    11161 tgataaatct ttagaccata tattcatatt agctaaggag aaaaatgttg aagttgaaca
    11221 aatggattta aaagcctata aatgttgtgg aataataaaa aatatgaaag atatttagga
    11281 aatatattag acaggagaat attgaatgat tgttttttca gatttagaca ggagtatcat
    11341 ttactccaat aaatttttaa atgctgatag taaatatact aatatagaaa tttataggga
    11401 aaaagaaata tcttatatat ctttagatac tataaattta ataaagcaaa tacaatatta
    11461 tggtatgttt atacctacaa ctactagaac agtggagcaa tttaagcgta tagaatttaa
    11521 taaatatggg atttattttc cttggagtat aacttcaaat ggaggagtta tacttaaaga
    11581 taatgaaata ttgaaatcat ggagtgaaaa aatagataaa ctaaaatgtg attatgagcc
    11641 cattgaaagt atgattcata aatttaaaga ctatttgaat gtagatggaa taactaattt
    11701 taaagttgca gaagatacat ttttttatat agttgttgat ttatcaagat ttaatttgga
    11761 ttcaatcaaa gaatatacaa atatattgga atcaaaaaat tggaagtttt atgtgagtgg
    11821 cagaaaaata tattttatac caaaagaaat ttcaaaagaa aatgctataa aatacttgac
    11881 taaagaatta ggaatagaat atttttatgc agttggagat tctattatgg actatggtat
    11941 gcttaatatc tcaaataaat cctatgtatt gaagcatggt gatataaata aaaatgaaat
    12001 agaaaattca tttatatcaa gttctttcaa tggaatgagt ggaactgaag agatactatc
    12061 taacatttta gacgagaact gcctacttaa tatataatga ctgatatata aataataaac
    12121 tagaaaaatc tgaaataaat tatagtaaag tttatcatac taatttattt cagattttta
    12181 tcaatttata tttaagtaag aactcaatat gttaaaatga aggtggagtc attgcccaga
    12241 ctgagataca tacctcatca tcataatttt catacacatg tggtatagta ctgttaaaat
    12301 aaaaactatc tccttcctcc aatagatatt ccttatttcc aagtgttatc ttcattttac
    12361 cttttattat atagccacat tcttctcctt cgtgtgtcac aaattcactg tttttttgtg
    12421 tatgaccatc tagtgtaaca agtataaatt ctattttttt cccagataaa ttaggaacaa
    12481 gaagttcata aaattttgta gaatcattag ttactatttt ttttcgatta tttttcttta
    12541 ctactatatc agagtcttct cttccaatct cttcaaaaaa ataacctata tttatatcaa
    12601 gagcttttga tattttccac attgctacaa tagatggaac aaccatatta cgctcaattt
    12661 gacttattaa gcctgtactt aaatctgaat tttttgctaa agtagagata cttatatttt
    12721 tagattttct aagagatcta attttgcttc caacatctat attcattaga tacaaacccc
    12781 ctcagacatg attataacat ataatttata aagattaata aaataaaaaa aaagttttac
    12841 aatacaagta ttttttatgc tatattgaat tttctgaaaa gatgcattat aatagtatta
    12901 atattcaata tattgaatat tgataacgat attttaattt aatgaaatgg aggtaattaa
    12961 gatgaagagt tttgtaagtc ctgataatat ttgggtatta tgggcagttg taactggatg
    13021 ggcagcattt agtatttatt tggaacaaaa gtacaattgg gcatcaaaag tgtcgggagc
    13081 aataatagct cttgtaggag caatgttatt atcaaatcta aatattatac cagtagagtc
    13141 tgttgtatat gaccaagtat ggggatatgt tgttccttta gccatagctt tattgttata
    13201 tcaatgtaac attaaaaaga tatggaaaga aagtggaaga ttgctcatta tatttcttgt
    13261 aggttctgta ggaactgtac ttggagctat gattggattt ttagcattaa aaaatgtagt
    13321 accagattta aatattgttg cagctatgat gacaggttct tatataggtg gaaatgtaaa
    13381 ctttgctgct atgtcagggg cgtttgatgc gccaggagaa ttagtatcag caacagttgt
    13441 agcagataat cttttaatgg cattatattt ctttgtctta atagctatac catcaattgg
    13501 attttttaga aaacatttta aacatccaca tgtagatgaa atggaaagta taggtataac
    13561 tgaaggagaa acagtagcag caaattattg gggaagaaaa gaaatatctt taaaagatat
    13621 agcaatagcc attggtagcg cctttataat tgttgcagta tctgtagaac tatcaacttg
    13681 gtttaaagct ataataccat tatcaaatcc ttttttagca atgttaaata ctttatttgg
    13741 aaatcaatat ctaataataa ctactttgac tatgttgttt gccacattta tgcctaattt
    13801 ctttggtgat ataaaaggag cacaggaatt gggtacattt ttaatatata tattctttgt
    13861 tgtagtaggt gttcctgctt caataacatt aataattcaa aaatcaccat tactactttt
    13921 atactgcgga ataatggtac taataaacat gctagttaca tttatagttg caaaaatatt
    13981 taagtttagt ttagaagaag ctattcttgc atctaatgca aatattggtg gtcctacaac
    14041 agcagcagct atggcaatat caaaaggatg gtctaagcta gtagggccaa tacttatagt
    14101 tggaacattt ggatacatag tcggtaatta ttttggtctt ttagtaggaa atattttgat
    14161 ataattttac gtgaaaaaaa gttgtttatt agaaagattt ttatttaatt ggaggttgtt
    14221 atgattttcg atggacattc agatacttgg gcagatgtaa caataagaag gcttaacggg
    14281 gaaaaagatg tatttagaag aatccatctt gataatttta aaagagcaaa tatagaagga
    14341 gctatttttg taatatggat agacccacca tatgataaag agcctgaaaa aagagttaaa
    14401 caaattataa gttctataaa agatgaaatt gaagatatga aagatgttgt aaacatagta
    14461 aaaaaatatt cagatataga taaaggtata agtgaaaaca aaataaatat tttaattggt
    14521 cttgaaggat taagccatat tggaagaaat atagatttac tagattattt ttataaagaa
    14581 gttggtgcaa gacatgcatc attaacttgg aatgaacaga atgaacttgc tacaggatgt
    14641 ttaggggatg aaaatcgtgg tttaagtgaa ataggcaaaa aagcagtcaa taaaatggaa
    14701 aaattgggca tgatagtaga tgtgtcacat ttaaatgaaa aatcattttg ggatttaatg
    14761 tctgttgcaa gtaagccagt tatagcttct cattctaatg ctaggagttt atgtgatgct
    14821 aaaagaaatc ttacagataa tcaattaaaa gaaatttcaa atacaggtgg attagttgga
    14881 ataaatagtt ttagaggatt tactaatagt aatcctaaga agcaagattt aatacatttg
    14941 gttgaccata tagactatat ggtaaatctt ataggtatag aacatgtatc ttttggtttt
    15001 gattttagtg attacttagg gaaagagact ataggttctt ttactggaaa tgctagtgtg
    15061 ccaagtgtag atggtcttga aagcacacat acagcacaga acatagtagt agagcttgaa
    15121 aatagaggtt ataaaaagaa agatatagag ttaatctcta gtggtaattt ttataggctc
    15181 tttaaagata tactaaaata atataataaa gaacttaaat ttataaaata gtatatttat
    15241 acaaataata tctacatatt taaattggcg ttcataattt attaaatttt aatgttacta
    15301 gaagtttcaa ttctagtaac attttttatg ttaaaaacag atatagatat gtattgacaa
    15361 tgataattat tattgataaa ctataaaagt gaaaatgata ataattttca ttttaattaa
    15421 taaaagattt ttaaggaggg agaatgaaga aaaggtattt agtcatagga ataataattt
    15481 tgtcttattt ttctttattt ataggagctg aagatataaa tataatgcat atatttgcca
    15541 aaaatcagca taaacttatg atatttatca tgagtagaat accaagatta ataagtatat
    15601 tgattgctgg tgtaggaatg agcatagcag gtcttataat gcaacagata agtaagaata
    15661 aatttgtatc acctacaaca ggtgctacaa tagatgcagc acaatttggg atagtaatat
    15721 gtatgctttt agtaccaact gcctctattt ttacaaagac aataatagca tttgtatttt
    15781 ctcttgtagg gacttttacg tttatgaaaa taatagggaa gttacaattt aaaaatataa
    15841 tctttgtacc attagtaggt ataatgtttg gaaatataat agggtctatg acggatttta
    15901 tcgcgtataa atacgatttg agtcaaaatg ttagttcttg gatgcaaggt gacttttcta
    15961 tgatacttaa aggaaattat gagatattat atatcacaat accccttata atattagcat
    16021 atatttatgc aaataaattt actgttgttg gtatgggtat ggattttgcg acaaacttag
    16081 gtcttagtta caaaagaatt gtcaatatag gtctgataat agtagcactt gttacagtat
    16141 gtgtagtcgt tacagctggt aatatccctt ttataggtct aatagttcca aatatcgtat
    16201 ccttgtacat gggtgataat ataagagcaa gcatatggta tactggttta cttggagcga
    16261 tatttgtact tatatgtgat atttttggaa gaattattat ttatccatat gaaatatcta
    16321 tagggcttac agttggagtt ataggaagta tactattcct ttatttaata ttaaggagga
    16381 atgtgaatga agcttagtct caatcaaaaa atttactttg taagtatatt actattcata
    16441 tcaatggcac tttttataat ttatggaata gacatgaatc atttagaata tgcattatca
    16501 caaagaatac caaaaatatt tgctatggta ttaggtggag gatgtatagc atttactact
    16561 gtagtgtttc aaacaattac aaacaatcaa atattaacac caagtgtact aggtttagat
    16621 tcattgtatg taatgattca aactataata gtattcatat ttggttcaag cagtagtctg
    16681 attataaatg aaaattataa ttttattatt aatgtagcta tgatgattgg agcttcattg
    16741 ttattatata agacgttatt tgaaaaaaat aaaaataata ttttcttttt aatcctagta
    16801 ggaatgatat ttggaacctt atttaaaagt gcaacaacat ttattcaggt aatgatagac
    16861 cctaatgaat ttttagcttt acaaactagt ataatggcaa gtttaaacaa tataaacact
    16921 aatgtattgt taatagcatt tataataatt attgccataa taccttttat atatgatgaa
    16981 ataaagtatt tagatgtatt gtcgttaggt aaagaacaag caattaattt aggtgtagat
    17041 tttgataaag cagttaaaaa aatgataata ttaatagcaa ttttagtctc aatatcaaca
    17101 gcacttattg gacctatgac atttttagga cttttacttg ctaatataac aagagaaatt
    17161 tttaaaacat ataagcatac atacttgatt tcgggttcta tgttgatagg gatgattaca
    17221 ttgataatag gtcaattttt tatacaacat gtatttaagt ttgatacaac tttaagtgta
    17281 gtaataaatt ttataggagg aatttatttt attcaattac tattaaaggg ggcaaataga
    17341 tgatagagat aaaaaatata tttaaaagat ataagaataa aaatgttgta gatgatgtct
    17401 cttttagtat agaaaaggga aagataacat cttttatagg accaaatgga gctggaaaaa
    17461 gtacagtact atcaatagtt acaagactta taggaggtga tggtggagaa gtaataatag
    17521 aaggaaaaag cttgactaat tatagcaata aagaattagc aaaaaaaata gctattctta
    17581 aacaatctaa caatataact ttaaagctta caataagaga attagttgga tttggaagat
    17641 ttccatatag tgaaggtaat ctaactaaag aggatgaaaa ttatattgat gaagcaatag
    17701 aatatatgaa gttgactgat atacaacata agtatttaga tgaattgagt ggaggtcaaa
    17761 gacaaagagc ttatatagca atggtaatag ctcaagatac agagtacata ttacttgatg
    17821 aaccactgaa caatttggat atgaaccatt ccgtacagat gatgaaggta cttagaggtc
    17881 tttgtgatga attagataag actatagtat tagtaatgca tgatataaat tttgcatcat
    17941 gttattcaga taatatagtt gcattaaaaa atggaaaagt tgaaaaagta ggtcgtacag
    18001 atgagatagt aaatgaaaaa gttttagaag atatttatga gatgaatttt aacattaaga
    18061 atataaatgg aaatcgaatc tgtatttatt tttaaaatat tgtatatgaa aataaacttg
    18121 ctatatgatt ttttataaag tttttaaatt aaaataaaga ttaataggag agaggaaaat
    18181 attgatatga ataaaaaagc agcaatagta gcagcagtag ctattatagg attagtgaca
    18241 gtgtttgcat taggtggaag taaaaagaat gaatcaaaaa catcagaaaa ttcaaataat
    18301 actatgaaga taactcataa cttgggagaa acagatgtaa aattaaatcc taaaaaagta
    18361 gtggtatttg attattcggc tttagataca atggatgcat taggtgtagc tgagacttta
    18421 gtaggacttc caaaagcgag tttaccagca tccttagaaa aatataagga tgacaagtat
    18481 gcagatttag gaggattaaa agaaccagat ttagaaggta taaagtcggc aaatccagat
    18541 ttaattataa taaatggaag acaagaagat ttttatgagc aattgtcaaa aatagcacca
    18601 acaataagta caagtaaaga tgataagaag tacttagaat ctgttaaaaa taatatagac
    18661 aaaatagcaa aaatatttgg agtagaagaa aaagcaaatc aagaatttag taaaattgaa
    18721 aagaaaatag aaactttgaa taaaaaagta acagataaaa atttaaatgc tttaactata
    18781 atggtaaatg aaggtaattt aagtgtattt ggtgaagaat caagatttag tatattatac
    18841 aatagttttg gatttgaaaa taaagataaa aatataaaag aatcaagtca tggacaaaat
    18901 attacttttg aatatatagc taaacaaaat ccagaagtta tgttcgttat agatagagga
    18961 atagctactg gcagtgatgt aaaagaaagc tcaactgcaa aatctgtttt gaacaatgat
    19021 ataataaaat ctatggatgc atataagaat gataatataa tatatttaga ttcaccaaca
    19081 tggtatgtaa atgatggtgg tctaacatct ttaaataaaa tgatagatga tgcttcaaaa
    19141 gcggtaaatt aataatccga tataaccaaa atgtattttt tataaaatga ttatcaaata
    19201 aactaggagt ttttaagaac tcctagttta ttttttgcat aaaaaagata tagtttaaac
    19261 aataatatta tctataataa ga
//
LOCUS       DAYAPA010000074        19024 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.074, whole genome shotgun sequence.
ACCESSION   DAYAPA010000074 DAYAPA010000000
VERSION     DAYAPA010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19024
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.074"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            129..1415
                     /locus_tag="ACGI59_002399"
     CDS             129..1415
                     /locus_tag="ACGI59_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006439082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HFG9740183.1"
                     /translation="MRLELGKIFIKDIQFGDVTEVKDGVLFINKQEMLQEIGGDEHIK
                     SIDIELARPGESVRITPVKDVIEPRVKVEGNGGIFPGIMSKVDTVGEGKTHALKGVAV
                     VTTGKIVGFQEGIIDMTGEGAKYTPFSKLNNVVVIAEPIDGLKQYAHEKAVRMIGFKA
                     AMYLGEVARNLTPDEVSVYETKPLLESIKEYPELPKVGYVYMLQTQGLLHDTYVYGVD
                     AKQIVPTLLYPTELMDGAIVSGNCVSACDKNPSYVHINNGVVEDLYARHGKDINFVGV
                     IITNENVYLADKERSSNWTSKLCKYLGLDAVIVSQEGFGNPDTDLIMNCKKIEMQGVK
                     TVIVTDEYAGRDGGSQSLADADVRADAVVTGGNANQVVVLPKLDKVIGHLEVVDVIAG
                     GSDGSLRADGTIEVEIQAITGATNETGFGHLTAKGY"
     gene            1527..2000
                     /gene="grdA"
                     /locus_tag="ACGI59_002400"
     CDS             1527..2000
                     /gene="grdA"
                     /locus_tag="ACGI59_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_110943085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030700 - glycine reductase complex
                     [Evidence IEA];
                     GO_function: GO:0030699 - glycine reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050485 - oxidoreductase activity, acting
                     on X-H and Y-H to form an X-Y bond, with a disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:1659..1661,aa:Sec)
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     selenoprotein A"
                     /protein_id="HFG9740184.1"
                     /translation="MSLLSNKKVLIIGDRDGIPGPAIEECVKTVEGAEVVFSSTECFV
                     UTAAGAMDLENQNRVKDAADKFGAENVVILLGAAEAEAAGLAAETVTAGDPTFAGPLA
                     GVALGLSVYHVVEEPIKSLFDESVYEDQISMMEMVLEVEEIEEEMSGIREEFCKF"
     gene            2032..3342
                     /gene="grdB"
                     /locus_tag="ACGI59_002401"
     CDS             2032..3342
                     /gene="grdB"
                     /locus_tag="ACGI59_002401"
                     /EC_number="1.21.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030700 - glycine reductase complex
                     [Evidence IEA];
                     GO_function: GO:0030699 - glycine reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050485 - oxidoreductase activity, acting
                     on X-H and Y-H to form an X-Y bond, with a disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:3079..3081,aa:Sec)
                     /transl_table=11
                     /product="glycine reductase complex selenoprotein B"
                     /protein_id="HFG9740185.1"
                     /translation="MGKLKAVHYINQFFAGIGGEEKADTKPHVAETLPPISLQLDKLL
                     GEDIEIVGTVVCGDSYFNENIDSASEEVLSMVKGFEPQLFIAGPAFNAGRYGVAAGTI
                     TKVVKDALNIPALTGMYVENPGADMFKKDVYVVETSDSAAGMRKALPKIAKLAVKLAN
                     GEEIGTPKDEGYIARGIRVNYFHEDRGSKRAVDMLVKKIKGEPFETEYPMPNFDRVDP
                     SKAVKDLSKCKIALVTSGGIVPKGNPDRIESSSASKYGTYSIAGVMDLTEETYETAHG
                     GYDPVYANLDADRVLPVDVLRDLEKEGVIGKLHETFYTTVGNGTSVANSKKYASEIGA
                     ALVADGVDAVILTSTUGTCTRCGATMVKEIEKTGLPVVHMCTVVPISLTVGANRIVPT
                     IAIPHPLGNPALDPTEEKALRRGLVEKALNALTTEVDGQTVFEK"
     gene            3598..5130
                     /gene="grdC"
                     /locus_tag="ACGI59_002402"
     CDS             3598..5130
                     /gene="grdC"
                     /locus_tag="ACGI59_002402"
                     /EC_number="1.21.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     component C subunit beta"
                     /protein_id="HFG9740186.1"
                     /translation="MTYPVLKGAGYVLIHTPDMIVQNGSTCTVERATNPDSEFLKEVS
                     NHIRSYEDVVNYMPNQVYIGNRRPEELRDLPMPWCEQKIEGTRNGKFGEIMPQDEFIA
                     LMQISDAFDLVKLSQEFIDEVKPKIENNYPEIAPFVGKLKGDDIEEGKELVATHIAEG
                     LYHDGKFVGYVKRAHDVDVNLNAHTMFENLVVKASGVLSAIQMLRHSKIDPAEIDYVI
                     ECSEEACGDINQRGGGNFAKSIAEIAGLQNATGSDTRGFCAAPTHALIQAAALVKAGI
                     HKNVMVVAGGASAKLGMNAKDHVKKGLPVLEDVVGGFAVLVSENDGVNPVIRTDLTGK
                     HTVGTGSSPQAVMTALITSGLDRANLKITDVDVYSVEMQNPDITKPAGAGDVPEANYK
                     MIGALAVKRGDLEKKELKDFVSNKGLPGWAPTQGHIPSGAPYIGFLIDDLTTGNRNRA
                     MIVGKGSLFLGRMTNLFDGVSFIAERNTGVTEETSGISKDEIKKIIAESMKKLALDML
                     EE"
     gene            5141..6286
                     /gene="grdD"
                     /locus_tag="ACGI59_002403"
     CDS             5141..6286
                     /gene="grdD"
                     /locus_tag="ACGI59_002403"
                     /EC_number="1.21.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     component C subunit alpha"
                     /protein_id="HFG9740187.1"
                     /translation="MSKKVIADVFLEVANAIESGEFGKKVKIGVTTLGSEHGVENMVN
                     GAQLAKSNLFDIVLIGPKVETDLEVVEVNDEKEMHAKMEELLDSGYIDACVTMHYNFP
                     IGVSTVGRVITPAKGKEMILATTTGTSATNRIEAMVRNAIYGIATAKSMGNKCPKVGI
                     LNVDGARQVEKCLKELKDNGYDMEFADSIRADGGCVMRGNDLLVGAPDVMVTDTLSGN
                     IFMKVFSSYTTGGDYEAQGFGYGPGVGEDYDRKVLIVSRASGSPVVANALKYAYDVVK
                     GDISNVARNEFAKVKKAKFDDIISSLTKKEVKAEKVEVKMPDKEIVTRQIAGVDIMDL
                     EDAVSELWKNGIYAESGMGCTGPIVLVNEAKGDLAVETLVKAGYTAK"
     gene            6596..7675
                     /locus_tag="ACGI59_002404"
     CDS             6596..7675
                     /locus_tag="ACGI59_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HFG9740188.1"
                     /translation="MSRVKNVVELLETKGVDALYLTKKTNVNYISGFPDEEAYAVICK
                     DGNFLVTDSRYMELAEKVCKDFEIINWHNFDRSVAKAVKSVCDKVGIKKLGFERTNIV
                     FDKYEELKNLIEKDNGELIPTENIVETLRYVKDKDEIKNTRKACEIADKALEELIPHI
                     KAGVSEIELATKLEYFMKMNGAQNIGFETILISGAKTSLLHGKPSDKIIEKGDFVLID
                     YGAMYNGYISDTTRTFIVGGASEKQLEIYNLVKEAQNVGVENMKAGVHATIPDAEIRK
                     VVKKYEDYYYQGIGHGVGRDVHEEPFIGNYGDKIIEEGCIITMEPGIYFPGWGGVRIE
                     DTVLITKNGPERLTKFPKDLMILDK"
     gene            7788..8387
                     /locus_tag="ACGI59_002405"
     CDS             7788..8387
                     /locus_tag="ACGI59_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9740189.1"
                     /translation="MYKKHKFIKNIKKAWYKIKLIDRCLIIVMAILMFQSIYNLFVNE
                     VNSQDTTTIDVVVRTTSAAIFGYFLSANFIKRPLRKYTNTDVSSNLFIDNNLSPKGQE
                     SNQNNIMNVKNTIGFTSEADNYEKKIPINNNEGFEEGETSELQIIIATVICIVALTVL
                     FVVRNFTTTTSASLGTISQMRDFVSGCVGFLLGCPSKPD"
     gene            complement(8573..9445)
                     /locus_tag="ACGI59_002406"
     CDS             complement(8573..9445)
                     /locus_tag="ACGI59_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFG9740190.1"
                     /translation="MNINYLYYFQTVCKYKNMTKAAESIHISQPSITLAIKELEKELG
                     FELFYRIGNKIELTPEGKIFLDKSKHFIKQFEDFQCDALDLGKKRKASLKIGIPTVLG
                     TFLLSKILPRFNVIYPDIELKIFEVPTFVGAKMIEESTLDFCIGIIDSDIYDDIDSKT
                     IYKTELYLVTNPKNELAKHPIISNYMLKNVPFVILSEGSYHYKIITKRLEKAKPNIIL
                     HSNQLSTIRYLLENDLASTILYKEIFQNTENLCSIPLERAITANIGVLWRRNQYISHS
                     MKLFIEYMASIHIN"
     gene            9862..11265
                     /locus_tag="ACGI59_002407"
     CDS             9862..11265
                     /locus_tag="ACGI59_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9740191.1"
                     /translation="MKGSDLVKFELMDFLMNPFVLMFAAVITGILFGKIKFGKFNFGV
                     SGALFTGLFIGWLAYSLGNLIIAKGETAAGYKAATVMMGNGIISSDFFDFFLIIFVAA
                     VGLLAAKDMKAVLKKYGARFVILGVLITFIGGSMTYAMTLLSSDKGSSAYEVSGVYTG
                     ALTSSPGLAAALETAGKHAEDVSKEFEKASIKDKKEILKVVDPEGKLDVNTTTSLTQE
                     QIDKYIAYAEAGVGIGHAVAYPFGVLIVILGVNFLPKLFRMDLKEERRKYEKEMKEAR
                     DSVSGKTIPEVPFNIITFFLTCLAGYLVGGIHVFMGPLGYFTLGATGGSLIVSLVLGY
                     IGKIGVVNFRMEEKVLNILKQIGLVFFLAIVGLRYGGKVVDSIMTSGMHLALVAIAVG
                     VTAMMIGFLVGKYVFKLNWILLSGAVCGGMTSTPGLGAAVDALDSDDPAAGYGATYPF
                     ALLTKVILVIVLHKLPM"
     gene            11292..12836
                     /locus_tag="ACGI59_002408"
     CDS             11292..12836
                     /locus_tag="ACGI59_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HFG9740192.1"
                     /translation="MNNEGAFEELSGRLRNKELCDLIMNADEAAKIIEDGMVIGVSGF
                     TPSGYPKAVPLAVSERAKSGEKIKLTVYSGASLGPEVDGAWSEAGIIERRLPYQTNSI
                     LRNNINKGVVDYIDMHLSHSTQFLNYGTIPKVDVAIVEALAITEEGNIIPTSGIGNSP
                     SFIKSADKVIVEINLAKPMEMEGMADIYITENPPNRKPIEINHPKDRIGTTYIPCGLD
                     KIAGVVITNMQDKTRPLGVVDEASKKISNNIIAFLREEVKAGRLSKNLLPLQSGVGSV
                     ANAVLYGLCESEFENLTCYTEVVQDSMLDLIRMGKVTMASTTSVSPSPEGLIKFEKDI
                     DFFKDKIILRPQEISNNPEIARRIGVIAMNTAIEVDIYGNVNSTHIMGSKMMNGIGGS
                     GDFARNGAITIFSTESIAKNGDISSIVPMVSHVDHTEHDVMVIVTEQGYADLRGLAPR
                     ERAIKIIENCAHPDYKEQLRDYLNRACKSGAKQTPHILDEALSWHSKFMSTGTMKKAE
                     TFKSAL"
     gene            12867..14258
                     /locus_tag="ACGI59_002409"
     CDS             12867..14258
                     /locus_tag="ACGI59_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HFG9740193.1"
                     /translation="MEKAVENFEDLSKEYINGYIERARKAQREFECYTQEQVDKIVKI
                     VGKVVYYNAEYLAKLAVEETGMGVYEDKVAKNKSKAKVIYNNLKDKKSVGIIDIDRET
                     GITKVAKPVGVVAAITPCTNPIVTPMSNAMFALKGRNAIIITPHHKAIGCSTKTVEMI
                     NEELEKIGAPENLIQILDQQSRENTRNLISSADVVIATGGMGMVKAAYSSGKPALGVG
                     AGNVQCIIDRDVDIKEAVPKIIAGRIFDNGIICSGEQSVIVAEEMFDKIMDEFKNNKG
                     FIVRDKVQKEAFRNAMFVNKSMNKDAVGQSVHTIAKIAGVEIPEDTKIIVIEADGPGE
                     EDIIAKEKMCPVISAYKYKSFEEGVAIAKANLNVEGKGHSVSIHSNTVKNIEYAGENI
                     EVSRFVINQCCATSAGGSFFNGLAPTNTLGCGSWGNNSISENLDYKHLINISRIAYYM
                     PENEVPTDEELWG"
     gene            14451..15947
                     /gene="abfD"
                     /locus_tag="ACGI59_002410"
     CDS             14451..15947
                     /gene="abfD"
                     /locus_tag="ACGI59_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybutyryl-CoA
                     dehydratase/vinylacetyl-CoA-Delta-isomerase"
                     /protein_id="HFG9740194.1"
                     /translation="MALMTGAQYIESLRKLNTKVYMFGEEVKNWVDHPMIRPSINCVA
                     ATYDLAHDPEYADLMTVTSNITGEKINRFGHLHQSVDDLIKKVKMQRLCGQKTASCFQ
                     RCVGMDAFNAVYSTTFECDKAHGTNYHDNFVKYLTYIQENDLVVDGAMTDPKGDRSLS
                     PSAQPDPDMFLHIVERREDGIIVRGAKAHQTGSINSHEHLIMPTISMTEADKDYAVSF
                     AVPSDAEGVFMIYGRQSCDTRKLEEGADVDLGNKEFGGQEALVVFDNVFVPNDRIFLC
                     GEWDFSGMLVERFAGYHRQSYGGCKVGVGDVIIGAAALAADYNGANKASHIKDKLIEM
                     THLNESLYCCGIACSSEGHKTEAGNYQIDLLLANVCKQNVTRFPYEIVRLAEDIAGGL
                     MVTMPSEKDFKSDLKVGTSGMTIGEVCNKYFKASSVASTEERMRILRFLENICLGSSA
                     VGYRTESMHGAGSPQAQRIMISRQGNINQKKELAKKIAGIKKEEALNL"
     gene            16082..16357
                     /locus_tag="ACGI59_002411"
     CDS             16082..16357
                     /locus_tag="ACGI59_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9740195.1"
                     /translation="MTCGVRFCGGCNPRYERGKALTIIKEHFNGRVDFSIAQENVEYD
                     LLLIIGGCATLCASYQQYKVKNECVKMWDFSHIERVINVIESAICET"
     gene            16381..17688
                     /locus_tag="ACGI59_002412"
     CDS             16381..17688
                     /locus_tag="ACGI59_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HFG9740196.1"
                     /translation="MSWQELYQSKLCSATEAVKQIKNGDTVVFAHCVGEPPALVEAMI
                     ENAEQYKDVEIKHMVSLGSGGYTAKGMEAHFRVNPMFVSGNVRKAIENGDGDFTPAFF
                     HEVPKLLREKRLKCDVVLAQVTPPDEHGYCSLGTSVDYTYEAIKTARTVIVQVNDQFP
                     RTYGEVVHVSEFDYIVEKSQPLFELQPAKIGEVEEAIGKNCASLIEDGSTLQLGIGGI
                     PDAVMLFLTDKKDLGIHSEMISDGTLALYEKGVINGKYKNFDKEKMTVTFLMGTKKLY
                     DFANNNPAVEVKPVDYVNHPAIIMKQHKMVSINSAIQVDLMGQVVAEAMGLRQFSGVG
                     GQVDFIRGVSMGEDGKAIIAMPSITTKKDGTVISKIVSIVDEGAPITTSRNDVDYIVT
                     EYGIAELKGKSLRERARNLINIAHPSVREHLAVEFEKRFKEKY"
     gene            17708..18826
                     /locus_tag="ACGI59_002413"
     CDS             17708..18826
                     /locus_tag="ACGI59_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybutyrate dehydrogenase"
                     /protein_id="HFG9740197.1"
                     /translation="MEALRVVPKIFYFDTFKEFNEEFKIGKNDLVITNEFIYEPYMKP
                     LGIDTNLIFQEKFGTGEPSDEMIDSMTKEMKKYNFDRIIAFGGGTIVDICKILALDVP
                     EKSIDLFEGEVSPKKVKELVVVPTTCGTGSEVTNVAIAELKSKHTKKGLAVEETYADY
                     AVLIPETVKGLPYKFFVTSSVDALIHAIESYLSPKASPFTEMYSLQAIKMIMDGYKKI
                     VDKGEEERFNHLRDFVLASNYAGIAFGNAGCAAVHALSYSIGGAFHVAHGEANYQFFT
                     EVFKMYSRKKPNGKIIKCTKILADALECDPNCDVYGELEKFLNKLIAKKALREYGMVE
                     SQIDEFTDSTIANQQRLLANNYVELSREEIREIFANLY"
BASE COUNT         7293 a         2166 c         3748 g         5817 t
ORIGIN      
        1 aaaataaaga tttaaggcat aaaaacacaa taatatatct ttaaaaaaat aagataaatt
       61 gtaaaatgat ataattttga gaaaaactca aatgcatata cacaaattaa ataacaggag
      121 gtgcaagtat gcgtcttgaa ttagggaaaa tctttataaa agatattcaa tttggtgatg
      181 taacagaagt aaaagatgga gtattattca tcaataagca agagatgcta caagaaattg
      241 gtggagatga gcatattaaa tctatagata tagaattagc tcgtccaggt gaaagcgtaa
      301 gaataacacc tgtaaaagat gttattgagc caagagtaaa agttgaaggt aatgggggaa
      361 tcttccctgg tatcatgtct aaagttgaca ctgtaggaga aggaaaaact catgctttaa
      421 aaggtgtagc tgttgtaact actggaaaaa tagtaggttt ccaagaaggt ataatagaca
      481 tgactggaga aggagctaag tacactccat tttctaaatt aaataatgta gtagtaatag
      541 ctgaacctat tgatggttta aaacaatacg ctcatgaaaa agctgttaga atgataggat
      601 tcaaagcagc aatgtactta ggagaagtag caagaaatct tactccagat gaagtttcag
      661 tatatgaaac taaacctctt cttgaatcaa taaaagaata tccagagctt ccaaaagtag
      721 gatatgttta catgcttcag actcaaggtt tattacatga tacttatgta tatggagtag
      781 atgcaaaaca aatagtacca actttattat atccaacaga attaatggac ggagcaattg
      841 taagtggtaa ctgtgtttct gcatgtgaca aaaacccaag ttatgttcat ataaacaatg
      901 gggttgttga agacttatat gcaagacatg gtaaagatat aaactttgta ggtgtaatta
      961 taactaatga aaatgtttat ctagcagata aagaaagatc ttcaaactgg acttctaaat
     1021 tatgtaaata tttaggatta gatgcagtta tagtttcaca agaaggtttt ggaaatcctg
     1081 atactgacct tataatgaac tgtaagaaga tagaaatgca aggtgttaaa acagttatag
     1141 ttacagatga atatgctgga cgtgatggag gttctcaatc acttgcagat gcagatgtaa
     1201 gagctgatgc tgttgtaaca ggtggaaatg ccaaccaagt tgtagttctt cctaagttag
     1261 ataaagttat tggacattta gaagtagtag atgtaatagc tggtggttct gatggaagtt
     1321 taagagctga tggaactatt gaagttgaaa tccaagctat aacaggtgca actaatgaaa
     1381 caggatttgg tcatttaact gctaaaggat attaagaagt attttattaa acgattgagt
     1441 aatgttaata aaacatatta tagataatta taataagtgt tatagatata taaaaatata
     1501 ttagaaatat aataaggagg attattatga gtttacttag taataaaaag gttcttataa
     1561 taggtgaccg tgatggtata ccaggacctg cgatagaaga atgtgtaaaa acagtagaag
     1621 gagcagaggt tgttttctca tctacagaat gctttgtctg aacagctgct ggggctatgg
     1681 acttagaaaa tcaaaacaga gttaaagatg ctgctgataa attcggagct gaaaatgttg
     1741 tgattttact aggtgctgct gaagccgaag ctgcaggtct tgcagccgaa acagtaactg
     1801 ctggagatcc aactttcgct ggaccacttg ctggagttgc cttaggatta agtgtttacc
     1861 acgttgttga ggaaccaata aaatcattat ttgatgaaag tgtatatgag gaccaaataa
     1921 gtatgatgga aatggtttta gaagttgaag aaatagaaga agaaatgtct ggtataagag
     1981 aagaattttg taaattttaa tgcctaaaag caaaaggggt gaaactaaga gatgggaaaa
     2041 ctaaaagcag tacattatat taatcagttc tttgctggta taggtggaga agaaaaagct
     2101 gatactaagc cacatgtggc agaaacttta cctccaataa gtttacaact tgataaatta
     2161 ttaggtgaag atattgaaat agttggaaca gtagtttgtg gagatagcta tttcaatgaa
     2221 aatatagata gtgcaagtga agaagtttta tcaatggtaa aaggttttga accacaactt
     2281 ttcatagctg gtcctgcatt taatgctggt agatatgggg ttgctgctgg aacaataact
     2341 aaagttgtaa aagatgcttt gaatatacca gctttaacag gtatgtatgt tgaaaatcca
     2401 ggagcagata tgttcaaaaa ggatgtttat gtagtagaaa cttctgattc tgcagcaggt
     2461 atgagaaagg cacttcctaa gatagctaaa cttgcagtaa aacttgctaa tggagaagaa
     2521 attggaactc ctaaagatga aggatacata gctagaggaa taagagttaa ctacttccat
     2581 gaagacagag gttctaagag agctgttgac atgttagtta agaaaataaa aggtgaacca
     2641 tttgaaactg aatacccaat gccaaacttt gatagagttg acccaagtaa agctgtaaaa
     2701 gatttatcaa aatgtaaaat agctttagtt acttctggtg gtatagttcc aaaaggaaat
     2761 ccagatagaa tagagtcttc ttcagcttct aaatatggaa cttattctat agcaggagtt
     2821 atggatttaa cagaagagac ttatgaaact gcacatggtg gatatgaccc agtgtatgca
     2881 aatttagatg cagatagagt attacctgtt gatgttctta gagatttaga aaaagaaggt
     2941 gtaattggaa aattacatga gactttctat acaactgtag gtaatggtac atctgttgca
     3001 aactctaaaa aatatgcttc tgaaattgga gcagcattag tggctgatgg tgtagatgca
     3061 gtaatattaa cttctacatg aggtacttgt actcgttgcg gtgcaacgat ggtaaaagaa
     3121 atagaaaaaa ctggacttcc agtagttcat atgtgtacag tagtacctat ttcattaaca
     3181 gtaggggcta atagaatagt tccaactata gcaatacctc atccacttgg aaatccagcg
     3241 cttgacccta cagaagaaaa agccctaaga agaggccttg tagaaaaagc attaaatgct
     3301 ttaactacag aagtagatgg tcaaacagta tttgaaaaat aaaaataaca cacacatata
     3361 ttatatagat aaaaatataa acacacacta aaaataaaaa ataactatac acaaaaaaat
     3421 aatatagatt tcatgaatgg ataaattgga gttattcatt catgaaatct taaaaaatgc
     3481 caaaatacat aaacaataat agaatcaaat atacaataaa atacaatgat tagagctttt
     3541 aataagttta ataagattaa aaaataagtg ttcataaaat ttatggaggt gcacaaaatg
     3601 acttatccag ttttaaaagg ggcaggctat gtacttattc atactccaga tatgatagtg
     3661 caaaatggaa gtacttgtac agttgaaaga gcaacaaatc cagattcaga atttttaaaa
     3721 gaagtaagta atcatataag aagttatgaa gatgtagtta actacatgcc aaatcaagtt
     3781 tatataggaa atagaagacc agaagaatta agagatttac caatgccatg gtgtgaacaa
     3841 aagattgagg gaactagaaa tggtaagttt ggagaaataa tgcctcaaga tgagttcata
     3901 gctttaatgc aaataagtga tgcatttgat ttagtaaaat tatcacaaga atttattgat
     3961 gaagtaaagc caaaaatcga aaataactac cctgaaattg caccatttgt aggtaaatta
     4021 aagggtgatg atatagaaga aggtaaagaa ttagtagcaa cacacatagc tgaaggatta
     4081 taccatgatg gaaaatttgt tggttatgta aaaagagcac atgatgtaga tgttaactta
     4141 aatgcccata caatgtttga aaatctagta gtaaaagcat ctggagtttt atctgctatt
     4201 caaatgctaa gacatagcaa aatagaccct gctgaaatag attatgttat tgagtgttca
     4261 gaagaagctt gtggagatat aaaccaaaga ggtggaggaa actttgctaa atcaatagct
     4321 gagatagcag gacttcaaaa tgcaactggt tctgacacaa gaggattttg tgcagcacca
     4381 actcatgcat taattcaagc agcagcttta gtaaaagctg gtatacataa aaatgtaatg
     4441 gtagtagcag gtggagcaag tgctaaactt ggaatgaatg ctaaagacca tgttaaaaaa
     4501 ggtcttcctg tacttgaaga tgttgttgga ggatttgcag tacttgtatc tgaaaatgat
     4561 ggtgtaaatc cagttataag aactgattta acaggtaagc atactgttgg aactggttct
     4621 tcaccacaag cagttatgac agcacttata acttcaggtc ttgatagagc taatttaaaa
     4681 ataacagatg ttgatgtgta ttcagttgaa atgcaaaacc cagatataac taagccagca
     4741 ggagcaggag atgttccaga agcaaactac aaaatgatag gggcacttgc tgttaaacgt
     4801 ggagacttag aaaagaaaga attgaaagat tttgtttcta acaaaggatt accaggatgg
     4861 gcaccaactc aaggacatat accatcagga gctccttata taggattttt aatagatgat
     4921 ttaactacag gaaatagaaa tagagctatg atagttggaa aaggtagttt attcttagga
     4981 agaatgacta acttatttga tggagtatct tttatagctg aaagaaatac aggtgttact
     5041 gaagagactt caggaatatc taaggatgaa attaagaaga taatagctga atctatgaaa
     5101 aaactagcat tagatatgtt agaagaatag gggtgaggat atgtcaaaaa aagtaatcgc
     5161 agatgtattc ttagaagtag ctaatgcaat agaaagcggc gaatttggca aaaaagtaaa
     5221 aattggtgtt actacacttg gaagtgaaca tggtgtagaa aacatggtta atggtgcaca
     5281 attagcaaaa tctaatttat ttgatattgt gcttattgga ccaaaagtgg aaactgacct
     5341 tgaagtagtt gaagttaatg atgaaaaaga aatgcatgca aaaatggaag aattattaga
     5401 ttcaggatat attgatgctt gtgttactat gcattataat tttccaatag gtgtatctac
     5461 agtaggtaga gtaataactc cagcaaaagg aaaagaaatg atacttgcaa caacaacagg
     5521 aacaagtgct actaatagaa tagaggcaat ggtaagaaat gccatttatg gtattgctac
     5581 agctaaatca atgggtaaca aatgcccaaa agttggaata cttaatgttg atggagcaag
     5641 acaagttgag aagtgcttaa aagaattaaa agataatgga tatgacatgg agtttgcgga
     5701 ttcaattaga gcagatggtg gatgtgtaat gagaggtaat gatttacttg taggtgcacc
     5761 tgatgttatg gttactgata cattatctgg aaatatattt atgaaagtat tttcttcata
     5821 tactacaggt ggagattatg aagctcaagg ctttggatac ggacctggag ttggagagga
     5881 ctatgataga aaggtactta tagtatcaag agcatcaggt tctccggtag tagcaaatgc
     5941 tttaaaatac gcttatgatg ttgtaaaggg agatataagt aatgttgcaa gaaatgaatt
     6001 tgcaaaggtt aaaaaagcaa aatttgatga cattatatcc tctcttacta aaaaagaagt
     6061 taaagctgaa aaagtagaag ttaaaatgcc agataaagaa attgttactc gtcaaatagc
     6121 tggtgttgat ataatggatt tagaagatgc agtatctgag ttatggaaaa acggaatcta
     6181 tgctgaaagt ggtatgggtt gtacaggtcc aatagtttta gtaaatgaag ctaaaggaga
     6241 cttagcagtt gaaactttag ttaaagctgg atatactgca aaataagtac attttttata
     6301 tgtaaataag aacatatagt tataatttta ttgattataa ttatatgttc tttttatttt
     6361 atatcttgtc aatatttata tattaacaca atccatttat ttgattctct agctaattta
     6421 ttatatttat tcagttaaaa ttaagctttc taactaaata aaaactaatg aagtattaaa
     6481 atgaaattga taaaatatga aaaaatttta ggaataaagt atctgttaga taaaaataag
     6541 attaattagt gatataatat aattaagata aaatttatta tttggaggca aaatcatgag
     6601 cagagtaaaa aatgtagtag aattgttgga aactaaaggt gttgatgcac tatatctaac
     6661 aaagaaaact aatgtaaact atataagtgg ttttcctgat gaagaagcat atgcagtgat
     6721 ttgcaaagat ggaaactttt tagttactga tagtagatat atggagcttg cagaaaaggt
     6781 ttgcaaagat tttgaaatta taaattggca taattttgat agaagtgtgg caaaggcagt
     6841 taaaagtgta tgtgataaag tgggtataaa gaaacttggg tttgaaagaa ctaatatagt
     6901 gtttgataaa tatgaagaac ttaaaaatct gattgagaag gataatggag aattaatacc
     6961 tacagaaaat attgtagaga ctttgagata tgtaaaagac aaagatgaaa tcaaaaatac
     7021 aagaaaagct tgtgaaatag cggataaggc attagaagaa ttgattccac atataaaagc
     7081 tggagttagc gaaattgaac tggctacaaa gttagagtac tttatgaaaa tgaacggagc
     7141 acaaaatata gggtttgaaa ctatactgat atcaggagca aagacttcat tgcttcatgg
     7201 taagcctagt gataagatta tagaaaaagg tgattttgta cttatagact atggcgctat
     7261 gtataatggc tatatatctg atacaactag aacttttata gttggaggag cttctgaaaa
     7321 gcaattagaa atatataatt tagttaagga agctcaaaat gtaggggttg agaacatgaa
     7381 agctggtgta catgcgacaa ttccagatgc tgagataaga aaagttgtca aaaaatatga
     7441 agactattac tatcaaggta taggacatgg agtcggtaga gatgttcatg aagaaccttt
     7501 cattggtaat tatggtgata aaatcataga agagggatgt ataataacaa tggaaccagg
     7561 tatttatttc cctggatggg gtggagttag aatagaagat actgttttga ttactaaaaa
     7621 tggtccagaa agacttacta aattccctaa agatttgatg attttagaca aataatttaa
     7681 tttatatata tttttttaca taaaatataa cataatctca ctattaagct ctatttacat
     7741 attatagtaa agagggctta attttattgt gagaggtgat agtatatatg tataaaaaac
     7801 ataaatttat aaaaaatata aaaaaagcct ggtataaaat caaattaatt gatagatgcc
     7861 ttataattgt aatggcaata ttaatgtttc aatctatata taatttattt gttaatgaag
     7921 taaattcaca agatactaca actattgatg tagtagtaag aaccacttca gcagctatat
     7981 ttgggtattt tttaagtgct aattttataa agagaccttt aaggaagtat acaaatactg
     8041 atgtatcttc aaatcttttt atagataata atttatcccc aaaaggtcaa gagtctaatc
     8101 aaaataatat aatgaatgtc aaaaatacca ttggatttac ttcagaagct gataattatg
     8161 aaaagaaaat acctattaat aataatgaag gttttgaaga aggagaaact agtgaattgc
     8221 aaattattat agctactgta atttgtatag tagcattaac agtacttttt gttgtaagaa
     8281 attttacaac tacaacatca gcttctttag gtactatttc acagatgaga gattttgtct
     8341 caggatgcgt gggttttttg ctaggttgtc caagtaaacc tgattaaaaa agtattatca
     8401 ataaaaggta ttttaaaaag tatcagtata ataatattta gatttaaatg tatacaatta
     8461 taattctaaa cattctatat tgtattttta tactgatact ctaaactata ctatttatta
     8521 aaatatttta acattgtatt taaacaaaat attatatctg tataatataa atttagttta
     8581 tatgaataga tgccatatac tcaataaaaa gtttcatact atgagatatg tattgatttc
     8641 ttctccaaag aaccccaata ttcgcagtaa ttgcacgttc taatggtatt gaacagagat
     8701 tttctgtatt ttgaaatatt tctttataca atattgttga agccaagtca ttttctagta
     8761 aataacgtat ggtagaaagt tgatttgagt gtaaaattat atttggctta gctttttcaa
     8821 gtctttttgt aataatctta tagtgataag aaccttcaga taagattaca aaaggtacat
     8881 tttttaacat ataattcgat ataatgggat gttttgccaa ttcatttttg ggatttgtaa
     8941 ctaagtataa ttctgtttta tagattgtct ttgaatctat atcatcataa atatcactgt
     9001 caattatacc aatacaaaaa tcaagtgtag attcttcaat cattttagct ccaacaaatg
     9061 ttggaacttc aaaaatttta agctctatat ctggataaat tacattaaat ctaggaagta
     9121 tttttgaaag caaaaacgta cctaaaactg taggaatacc tattttgaga gaagcttttc
     9181 tcttttttcc taaatcaagt gcgtcacatt gaaaatcctc aaattgtttt ataaaatgtt
     9241 ttgacttgtc taaaaaaatt tttccttcag gcgttaactc tattttatta cctattctgt
     9301 aaaaaagttc aaagccaagt tctttttcta attctttaat tgctaatgtt atggatggtt
     9361 gagaaatatg aatactttcc gcagccttag tcatgttttt atatttacag acagtttgaa
     9421 aatagtataa atagtttata ttcatttggt attagttcct ttcatttaca tattagtgac
     9481 aagtatataa ttaacaatag cataagtttt gtatattgtt gtaaaaaaat agaatagttt
     9541 taaactatca tagcataagt ttttgatatt ttcaatctat taagaatagt gctattatta
     9601 aattaacaaa agagaatagc tggtatggtt tagagtaaat caatctatat tacagttata
     9661 tcaacgaata tcaaaaaatt atctattaga aaataaaaat tatttttaca attaaattgt
     9721 taattaaatc ataaagaaaa aagtgatatt aacaagataa aattttttta atcagaaatg
     9781 ttaatttaat aacaaaatag agtattgggg agaaaggaga aatatatttt ttaataatat
     9841 ttgttaattt aataacgaaa aatgaaaggg agtgatttag tgaagtttga attgatggat
     9901 tttttaatga atccttttgt ccttatgttt gctgcagtaa ttacaggaat tttatttgga
     9961 aaaattaaat ttgggaagtt taattttggg gtatcaggag cattatttac aggattattt
    10021 attggatggc ttgcatatag cttggggaat ctaataatag caaaagggga aacagcagca
    10081 ggatataagg cagcaactgt aatgatgggt aatggaatta tttcttctga cttctttgat
    10141 ttctttttaa taatatttgt tgcagcagtt ggtctattgg cagcaaaaga tatgaaagct
    10201 gtattaaaga agtatggagc aagatttgta attttaggag ttcttataac ttttataggt
    10261 ggctctatga cttatgcaat gactttacta tcaagtgata aaggaagtag tgcttatgaa
    10321 gtttctggcg tttacacagg agcattaaca agttcgcctg gattagctgc tgctttagaa
    10381 acagctggta aacatgcaga agatgtatca aaagaatttg aaaaagcttc aataaaagat
    10441 aaaaaagaaa ttttaaaagt agtcgaccca gaaggcaaat tggatgtgaa tacaactaca
    10501 tcactgactc aagagcaaat agataagtat attgcatatg ctgaagctgg tgttggtata
    10561 ggtcatgcag tcgcatatcc ttttggagtt ttaatagtaa tcttaggtgt aaatttcctt
    10621 ccaaaattat ttaggatgga cctaaaagaa gaaagaagaa aatatgagaa ggaaatgaag
    10681 gaagcaagag atagtgtttc tggtaaaacg ataccagaag ttccatttaa tataataaca
    10741 tttttcttga catgtttagc tggatactta gttggaggaa tacatgtatt tatgggacca
    10801 ctagggtact tcacactagg cgcaacagga ggttcactaa ttgtatctct agttcttgga
    10861 tatataggta agataggggt agtaaatttc agaatggaag aaaaggtttt aaatatttta
    10921 aaacaaatag ggctagtatt tttcttagca atagttggat taagatatgg tggaaaagta
    10981 gttgattcaa ttatgacatc tggtatgcac ctagccttag tagcaatagc agtaggtgtt
    11041 acagctatga tgataggatt cttagttggc aaatacgtat ttaaattaaa ttggatttta
    11101 ttatctggag ctgtctgtgg tggtatgaca tcaacaccag gtcttggagc tgctgttgat
    11161 gctcttgata gtgatgaccc agcagcaggt tatggtgcta cgtatccatt tgcattatta
    11221 actaaagtta tattggttat agttttacac aaacttccaa tgtaaataag attttatatg
    11281 gggggatatt tatgaataat gaaggagcat ttgaagaatt aagtggaaga ttaagaaata
    11341 aagaattgtg tgatttaata atgaatgcag atgaagctgc taaaattata gaggatggca
    11401 tggttattgg agttagtgga tttacacctt caggctatcc aaaagcagta ccattagcag
    11461 tttcagaaag agctaaaagt ggagaaaaga taaaattaac cgtatattca ggagcatctt
    11521 taggtccaga agttgatgga gcatggtcag aagcaggtat aattgaaaga agacttcctt
    11581 atcaaactaa ttcaatactt agaaataata ttaataaagg tgttgttgat tatatagata
    11641 tgcacctaag tcattctact caatttttaa attatggaac tatacctaaa gtagatgtag
    11701 ctatagtaga agcacttgca ataactgaag aaggaaatat tataccaact agtgggatag
    11761 gtaattctcc atcttttata aaaagtgcag ataaagtaat agtagagata aatcttgcaa
    11821 aaccaatgga aatggaaggt atggcagata tatatattac ggaaaatcca ccaaatagaa
    11881 aaccaataga aataaatcat cctaaggata gaattggaac aacatatata ccttgtggtt
    11941 tggataaaat tgcaggagta gtaattacca atatgcaaga taagacccgt ccattgggag
    12001 ttgtagatga agcttcgaaa aaaatatcaa ataatattat tgctttttta agagaagaag
    12061 taaaggcagg cagattatca aagaatttac ttcctcttca atctggtgta ggaagtgtag
    12121 caaatgcagt attatatgga ttatgtgaat ctgaatttga aaatttaact tgttatacag
    12181 aggttgtaca ggactctatg ttggatttaa taagaatggg taaagttaca atggcatcta
    12241 ctacatctgt aagtccatct ccagaaggat taataaaatt tgaaaaagat attgattttt
    12301 ttaaggataa gattatatta agacctcaag aaattagtaa caatccagaa atagccagaa
    12361 gaattggtgt aattgcaatg aacactgcaa ttgaagttga tatttatgga aatgtaaatt
    12421 ctactcacat aatggggtca aagatgatga atggaattgg tggttctggt gattttgcta
    12481 gaaatggagc aataactata tttagtactg agtcaattgc aaaaaatggt gatatatcat
    12541 caatagtacc tatggtatct cacgtagacc acactgaaca tgatgtaatg gtaattgtaa
    12601 cagaacaagg atatgctgat ttaagaggtc ttgcacctag agaaagagct ataaaaataa
    12661 tagaaaattg tgcccatcca gattataaag aacaacttag ggattattta aatagagctt
    12721 gtaaaagtgg agcaaaacaa acacctcaca tattggacga agctttatca tggcatagta
    12781 aatttatgag tacaggtaca atgaaaaagg ctgaaacttt taaaagtgca ttataaaata
    12841 tttaataaat tggaggtaga ttttaaatgg aaaaagcagt ggaaaacttt gaagatttaa
    12901 gtaaggaata cattaatggt tatattgaga gagcaagaaa agctcaaaga gagtttgaat
    12961 gttatactca agaacaagta gataaaatag taaaaatagt tggaaaagta gtgtattata
    13021 atgctgaata tttagctaaa cttgctgtag aagagactgg aatgggagta tatgaagata
    13081 aggtagctaa aaataaaagc aaagcaaaag ttatatataa caatttaaaa gataaaaaat
    13141 cagttggtat aattgatata gatagagaaa ctggaataac aaaggtagct aaaccagttg
    13201 gagtagtagc ggcaataact ccttgtacaa atccaatcgt tacacctatg agcaatgcaa
    13261 tgtttgcact taagggaaga aatgcaatta ttatcacacc tcatcataaa gcaattggat
    13321 gtagtacaaa aactgtagag atgattaatg aagaattaga aaaaatagga gctccagaaa
    13381 atttaataca aatacttgac caacaatcaa gagaaaatac aagaaattta atttcatcag
    13441 cagatgtagt aattgccact ggaggaatgg gaatggtaaa agctgcgtac tcaagtggaa
    13501 aaccagcact tggtgtaggg gcaggaaatg ttcaatgtat catagataga gatgtagata
    13561 ttaaagaagc agtacctaaa atcatagcag gaagaatttt tgataatggt atcatttgtt
    13621 caggtgaaca gtcagtaata gtagcagaag aaatgtttga taaaataatg gatgaattta
    13681 aaaataataa aggttttata gttagagata aagtacaaaa agaagctttc agaaatgcta
    13741 tgtttgtaaa taaatctatg aataaagatg cagtaggtca atcagttcat acaattgcaa
    13801 aaattgcagg tgttgagata ccagaagata caaaaataat agtaatagaa gctgatggac
    13861 caggagaaga ggatattatt gcaaaagaaa aaatgtgtcc agttatatct gcatacaagt
    13921 ataaaagttt tgaagaaggt gtagctattg caaaagcaaa tttaaatgta gaaggaaaag
    13981 gacatagtgt atctatacat tcaaatacag ttaaaaatat agaatatgca ggagaaaata
    14041 tagaagtatc aagatttgtt attaatcaat gttgtgctac tagtgctgga ggaagtttct
    14101 ttaatggtct tgcacctact aatactctag gttgtggttc ttggggaaat aacagcatct
    14161 ctgaaaatct tgattataag catctaatta atatttctag aattgcttat tatatgccag
    14221 aaaatgaagt ccctacagat gaagaacttt ggggataaaa tttagacaaa aaatattcat
    14281 tctaaattaa agtaggaata tattttaatt tagaatgaat ttaagacata aattaaattt
    14341 aaattagttt attggactaa atttaaaata ttaagagaca tatagaagaa ggaagtatta
    14401 taatttaaaa taaataaaaa aatctataaa agagaaggag tggttttatt atggcattaa
    14461 tgacaggagc tcaatatatt gaaagtttga gaaaattaaa tactaaggtt tatatgtttg
    14521 gtgaagaggt taagaattgg gttgaccatc ctatgattag accatctata aattgtgtag
    14581 cagcaactta tgacttagct catgacccag aatatgcaga tttaatgaca gttacttcaa
    14641 atatcacagg tgaaaaaata aatagatttg gtcatcttca tcaaagtgtt gatgatttga
    14701 taaaaaaagt aaaaatgcaa agactatgtg gtcaaaaaac agcttcttgt ttccaaagat
    14761 gtgttggtat ggatgcgttt aatgcagtgt attcaacaac atttgagtgt gataaagccc
    14821 atggaactaa ttatcatgat aattttgtaa aatacttaac ttatatacaa gaaaacgatt
    14881 tagtagttga tggagctatg actgacccta agggagatag aagtctatca cctagtgctc
    14941 aacctgaccc agatatgttc cttcatatag tagaaagaag agaagatggg ataattgtaa
    15001 gaggagcaaa ggcacatcaa actggttcaa taaattcaca tgagcacctt ataatgccta
    15061 ctataagtat gactgaagca gataaagact acgctgtatc atttgcagtt ccttcagatg
    15121 ctgaaggagt gtttatgatt tatggaagac aatcttgtga cactagaaaa ttagaagaag
    15181 gtgcagatgt tgatttagga aataaggagt ttggaggtca agaagcttta gtagtatttg
    15241 ataatgtatt tgttccaaat gatagaattt tcttatgtgg agaatgggat ttctctggta
    15301 tgttagtaga gagatttgca ggataccata gacaaagtta tggtggatgt aaagtaggag
    15361 taggtgatgt aataatagga gcagcagctt tagcagctga ttataatgga gcaaacaaag
    15421 cttcacatat aaaagataaa ctaatagaaa tgactcacct taatgaaagc ttatattgct
    15481 gtggtatagc atgttcatca gaaggtcaca aaacagaagc tggaaactat caaatagatt
    15541 tacttcttgc aaatgtgtgt aaacaaaatg taactagatt tccttatgaa atagtaagac
    15601 ttgcagaaga tatagctgga gggcttatgg ttactatgcc ttcagaaaaa gactttaaat
    15661 cagaccttaa agttggaaca agtggaatga ctattggcga agtatgtaac aagtatttta
    15721 aagcttcttc agtggcttca acagaagaaa gaatgagaat attaagattc ttagaaaata
    15781 tatgccttgg ttcatctgct gttggttata gaactgagtc aatgcatggg gcaggttcac
    15841 cacaagctca aagaataatg atttcaagac aaggaaacat aaatcaaaag aaagaattag
    15901 ctaagaaaat tgcgggaata aaaaaagagg aagctttaaa tttataaaaa gttgatatat
    15961 aaagggaaat tatatattaa tgtgtgttaa ttaatgaaaa aatatgtagt ataagagata
    16021 gttataaaat tatggtggtt tttagctaag taaagaaatt tttaataagg agaatttaca
    16081 catgacctgt ggagttagat tttgtggggg ctgtaaccct agatacgaaa gagggaaggc
    16141 tcttacaata atcaaagagc acttcaatgg tagggtagat tttagtatag cacaagagaa
    16201 cgtcgagtat gatttgttac ttataattgg aggatgcgca actctatgcg catcctatca
    16261 acaatataaa gtgaaaaatg aatgtgttaa gatgtgggat tttagccata tagaaagagt
    16321 tataaatgtg attgaatcag caatttgtga aacttaaaaa agctaaatgg ggggatttca
    16381 ttgagttggc aagaattgta tcaaagtaaa ttatgtagtg ctacagaagc tgtaaaacag
    16441 attaaaaacg gtgatactgt agtatttgca cattgtgtag gtgaacctcc tgcactagta
    16501 gaagctatga ttgaaaatgc tgaacaatat aaagatgttg agattaaaca tatggtaagc
    16561 ttgggtagtg gtggatatac tgctaaggga atggaagctc attttagagt aaatccaatg
    16621 tttgtaagtg gaaatgtaag aaaggcaatt gaaaatggag atggtgattt tacacctgca
    16681 ttcttccatg aagtaccaaa gttgcttaga gaaaaaagat taaaatgtga tgttgtttta
    16741 gcacaggtaa ctccaccaga tgaacatggg tattgtagct taggaacatc agttgattat
    16801 acttatgaag ccattaaaac tgctagaaca gtaattgttc aagtgaatga ccagtttcct
    16861 agaacctatg gtgaggtagt ccatgtatca gagtttgact atatagtgga aaaatcacaa
    16921 cctttatttg aactacaacc tgcaaagatt ggagaagtag aagaagcaat tggtaaaaat
    16981 tgtgcttcat taattgaaga tggctctact ttacagctag ggattggtgg gataccagat
    17041 gcagttatgt tatttttaac tgataaaaag gatttaggga ttcattcaga aatgatttca
    17101 gatgggactt tggcccttta tgaaaaaggt gttataaatg gtaaatataa aaattttgat
    17161 aaagaaaaaa tgacagttac cttcttaatg ggtactaaaa aattatatga ctttgcaaat
    17221 aataaccctg cagtagaggt aaaaccagta gactatgtga atcatccagc aattataatg
    17281 aaacaacata agatggtttc tataaattct gctatacaag ttgatttaat gggacaggta
    17341 gttgcagagg ctatgggact tagacaattt agtggtgttg gaggtcaagt tgactttata
    17401 cgtggagtgt ctatgggtga agatggaaag gcaattatag ctatgccttc aataactaca
    17461 aaaaaagatg gtacagtaat ttcaaaaatc gtatctattg tggatgaagg tgcacctata
    17521 acgacatcaa gaaatgatgt tgactatatt gtaacagaat acggtatagc agaattaaaa
    17581 ggaaaatctc ttagagaaag agcaagaaat ttaataaata tagctcatcc aagtgtaaga
    17641 gaacatctag cagtagaatt tgaaaagaga tttaaggaga aatattaata atagaggggt
    17701 gttaataatg gaagcactta gagtagttcc aaagatattt tactttgata catttaaaga
    17761 atttaatgaa gaatttaaaa taggaaaaaa tgatttagta attactaatg agtttatata
    17821 tgagccatat atgaaacctc ttggaataga tacaaattta atttttcaag aaaagtttgg
    17881 aacaggtgaa ccatctgatg aaatgataga ctccatgaca aaggaaatga agaaatacaa
    17941 ttttgataga attattgcat ttggaggagg aacaatagtt gatatatgta agatattagc
    18001 acttgatgtt ccagaaaagt ctattgattt atttgaagga gaagtatcac ctaaaaaagt
    18061 aaaagagtta gtagtagttc ctacaacatg tggtacaggt agtgaagtaa caaatgtagc
    18121 tattgcagag ttaaaatcta aacatactaa aaaggggctt gcagtagaag aaacatatgc
    18181 agactatgca gtgttaattc cagagacagt aaaagggtta ccttacaaat tctttgttac
    18241 aagttcggta gatgctctta tacatgctat tgagtcttat ctttctccaa aagcaagtcc
    18301 atttacagag atgtattctc tacaagcaat aaaaatgatt atggatgggt ataagaagat
    18361 agttgataaa ggagaagaag agagatttaa tcatcttaga gactttgtac ttgcatcaaa
    18421 ttatgcagga attgcttttg gaaatgctgg ttgtgcagca gttcatgcac tatcttattc
    18481 aataggtgga gctttccatg ttgcacatgg tgaagcaaat tatcagttct ttactgaagt
    18541 atttaagatg tattctagaa aaaaacctaa tggaaaaatt ataaaatgta caaaaatttt
    18601 agcagatgcc cttgaatgtg accctaactg tgatgtttat ggagaattag aaaagttttt
    18661 aaataaatta atagctaaaa aagctttgag agaatatgga atggtggaaa gtcagataga
    18721 tgaatttaca gatagtacaa tagcaaatca acaacgacta cttgctaata attatgtaga
    18781 gcttagtaga gaggaaataa gagagatatt tgcaaatctt tattaataca attttttatt
    18841 tatatgtata gtcaacataa acaatgctaa ttatatttat gtacaaataa aaataaggga
    18901 acttaaattt taattaaagt taagttctct tatttttata tggagttaaa tataagtata
    18961 tttttaattt aaaatatgct tatatttaag tctattactt tatttgaaac aatttgtaga
    19021 attt
//
LOCUS       DAYAPA010000075        18783 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.075, whole genome shotgun sequence.
ACCESSION   DAYAPA010000075 DAYAPA010000000
VERSION     DAYAPA010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18783
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.075"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(66..245)
                     /locus_tag="ACGI59_002414"
     CDS             complement(66..245)
                     /locus_tag="ACGI59_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9740198.1"
                     /translation="MLDRFFGEEGCFGGGGWWIIVLFFLFLAFGDSWAEFDIMAWIPF
                     LIVLLILCCCGGCLE"
     gene            complement(427..633)
                     /locus_tag="ACGI59_002415"
     CDS             complement(427..633)
                     /locus_tag="ACGI59_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9740199.1"
                     /translation="MTKLEDNMERFFGGCEGFFGNKMLIIIVIVFLLLCTDILEDLLC
                     DDNIWIWVILIVLLLFNFDDGCCC"
     gene            complement(681..1106)
                     /locus_tag="ACGI59_002416"
     CDS             complement(681..1106)
                     /locus_tag="ACGI59_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9740200.1"
                     /translation="MKYVGLLLTSVGMFLLIAVNFYYNSITLDMQRIEDYVMETNLIL
                     EDVAEKESYVSNEKEDYISRLMHVKKGIENSKTSFLIERYKEYKIKSIESLIYTISEE
                     KKDYLDEVDRYNKLGEKEINKLINKNFLEVTYLSITTYI"
     gene            1278..1505
                     /gene="acpP"
                     /locus_tag="ACGI59_002417"
     CDS             1278..1505
                     /gene="acpP"
                     /locus_tag="ACGI59_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFG9740201.1"
                     /translation="MFEKIKEIIAEQLGVDNLDEITMEASLMDDLEADSLDAVEIIMA
                     LEDEFGIEIPDEEAENFKCIGDICKYIEENK"
     gene            complement(1649..2653)
                     /locus_tag="ACGI59_002418"
     CDS             complement(1649..2653)
                     /locus_tag="ACGI59_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HFG9740202.1"
                     /translation="MIIHKFIIHVLDKNSDVPILNDFEGKVNQEVDGFFQKAIKRIAK
                     DEDLRKGVFKDYNDNLIKNCCEQIIYDESTFLKNSKEIASYLFDVMKINAALESCDLA
                     ICLYTIKDEKSVAILKLDYKKLYTHSIEFVDDKFNIQMVSNEIGIPETLRQKQGALIS
                     LSGINDEFHLRLLDKDAEKEGSESKFVTEFLNAKKIDDDKYKTKVFKNTAENWITNAL
                     SNDIKQAEDVRSILNYTLKEKHEVDINDFVDNSIKDDELKDSFKEHMEEKGLDESFSI
                     DKKWVEKKLKKRSIKTDNGFDIKGNLTDFEDPMKYTVKQNQDGTIDIIIKNVTFYEEK
                     "
     gene            complement(2667..3569)
                     /locus_tag="ACGI59_002419"
     CDS             complement(2667..3569)
                     /locus_tag="ACGI59_002419"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFG9740203.1"
                     /translation="MFKKENQRYNLISYTNEEEMTLKEVLLDKLNFSVRSLSKMKREK
                     SVLVNGVYKKPSLKVYSGDLIEVKIDEEKANFEPQDLNLQIIYDDFDIIMVNKPPFMV
                     VHPTKSHYDKTIANGISYYIDNQKENVKIRFVNRLDMNTSGLVIVAKNAYAHHTLSTA
                     MSENKVEKKYITVVDGIIKENEGTIDEPIYRPTEDSIKRIIDERGQSSVTHYKVIERL
                     ENATVLEVSLETGRTHQIRVHMAHIGHGIIGDELYGYVDEELINRQALHAYKLEFEQP
                     RTKEKLKFKADIPEDMKELISKLR"
     gene            complement(3764..4939)
                     /locus_tag="ACGI59_002420"
     CDS             complement(3764..4939)
                     /locus_tag="ACGI59_002420"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HFG9740204.1"
                     /translation="MREVVIASAARTAVGSFGGAFKSVSAVELGVTAAKEAIKRANIT
                     PDMIDESLLGGVLTAGLGQNIARQIALGAGIPVEKPAMTINIVCGSGLRSVSMASQLI
                     ALGDADIMLVGGAENMSMSPYLVPSARYGARMGDAAFVDSMIKDGLSDIFNNYHMGIT
                     AENIAEQWNITREEQDELALASQNKAEKAQAEGKFDEEIVPVVIKGRKGDTVVDKDEY
                     IKPGTTMEKLAKLRPAFKKDGTVTAGNASGINDGAAMLVVMAKEKAEELGIEPLATIV
                     SYGTAGVDPKIMGYGPVPATKKALEAANMTIEDIDLVEANEAFAAQSVAVIRDLNIDM
                     NKVNVNGGAIAIGHPIGCSGARILTTLLYEMKRRDAKTGLATLCIGGGMGTTLIVKR"
     gene            complement(5026..5871)
                     /locus_tag="ACGI59_002421"
     CDS             complement(5026..5871)
                     /locus_tag="ACGI59_002421"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HFG9740205.1"
                     /translation="MKLAVIGSGTMGSGIVQTFASCGHDVCLKSRTQGAIDKCLALLD
                     KNLTKLVTKGKMDEATKAEILSHVSSTTNYEDLKDMDLIIEASVEDMNIKKDVFKLLD
                     ELCKEDTILATNTSSLSITEIASSTKRPDKVIGMHFFNPVPMMKLVEVISGQLTSKVT
                     FDTVFELSKSINKVPVDVSESPGFVVNRILIPMINEAVGIYADGVASKEEIDEAMKLG
                     ANHPMGPLALGDLIGLDVVLAIMNVLYTEFGDTKYRPHPLLAKMVRANQLGRKTKIGF
                     YDYNK"
     gene            complement(5969..6766)
                     /locus_tag="ACGI59_002422"
     CDS             complement(5969..6766)
                     /locus_tag="ACGI59_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HFG9740206.1"
                     /translation="MSTSDVKVYENVAVEVDGNICTVKMNRPKALNAINSKTLEELYE
                     VFVDINNDETIDVVILTGEGKAFVAGADIAYMKDLDAVAAKDFSILGAKAFGEIENSK
                     KVVIAAVNGFALGGGCELAMACDIRIASAKAKFGQPEVTLGITPGYGGTQRLTRLVGM
                     AKAKELIFTGQVIKADEAEKIGLVNRVVEPDILIEEVEKLAKIIAKNAQLAVRYSKEA
                     IQLGAQTDINTGIDIESNLFGLCFSTKDQKEGMSAFVEKREANFIKG"
     gene            complement(6785..7795)
                     /locus_tag="ACGI59_002423"
     CDS             complement(6785..7795)
                     /locus_tag="ACGI59_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFG9740207.1"
                     /translation="MGNVLVVIEQRENVIQTVSLELLGKATEIAKDYDTKVSALLLGS
                     KVEGLIDTLAHYGADEVIVVDDEALAVYTTEPYTKAAYEAIKAADPIVVLFGATSIGR
                     DLAPRVSARIHTGLTADCTGLAVAEDTKLLLMTRPAFGGNIMATIVCKDFRPQMSTVR
                     PGVMKKNEPDETKEAVINRFKVEFNDADKLVQVVQVIKEAKKQVKIEDAKILVSAGRG
                     MGGKENLDILYELAEIIGGEVSGSRATIDAGWLDKARQVGQTGKTVRPDLYIACGISG
                     AIQHIAGMEDAEFIVAINKNPEAPIFKYADVGIVGDVHKVLPELISQLSVAKEKGEVL
                     AN"
     gene            complement(7814..8596)
                     /locus_tag="ACGI59_002424"
     CDS             complement(7814..8596)
                     /locus_tag="ACGI59_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009524639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HFG9740208.1"
                     /translation="MNIVVCIKQVPDTTEVKLDPNTGTLIRDGVPSIINPDDKAGLEE
                     AIKLKEEMGAHVTVITMGPPQADMALKEALAMGADRGILLTDRAFAGADTWATSSALA
                     GALKNIDFDIIIAGRQAIDGDTAQVGPQIAEHLNLPSITYAEEIKTEGEYVLVKRQFE
                     DCCHDLKVKMPCLITTLKDMNTPRYMKVGRIYDAFENDVVETWTVKDIEVDPSNLGLK
                     GSPTSVFKSFTKSVKPAGTIYNEDAKTSAGIIIDKLKEKYII"
     gene            complement(8655..9791)
                     /locus_tag="ACGI59_002425"
     CDS             complement(8655..9791)
                     /locus_tag="ACGI59_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009272751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HFG9740209.1"
                     /translation="MDLNSKKYQMLKELYVSFAENEVKPLATELDEEERFPYETVEKM
                     AKAGMMGIPYPKEYGGEGGDTVGYIMAVEELSRVCGTTGVILSAHTSLGSWPIYQYGN
                     EEQKQKFLRPLASGEKLGAFGLTEPNAGTDASGQQTTAVLDGDEYILNGSKIFITNAI
                     AGDIYVVMAMTDKSKGNKGISAFIVEKGTPGFSFGVKEKKMGIRGSATSELIFEDCRI
                     PKENLLGKEGQGFKIAMSTLDGGRIGIAAQALGLAQGALDETVKYVKERVQFGRPLSK
                     FQNTQFQLADMEVKVQAARHLVYQAAINKDLGKPYGVEAAMAKLFAAETAMEVTTKAV
                     QLHGGYGYTRDYPVERMMRDAKITEIYEGTSEVQRMVISGKLLK"
     gene            complement(10500..10967)
                     /locus_tag="ACGI59_002426"
     CDS             complement(10500..10967)
                     /locus_tag="ACGI59_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HFG9740210.1"
                     /translation="MSISNVREYFKQFGKEDSILEFEQSSATVELAAEAAGVIPARIA
                     KTLSFKIGDDAILIVTAGDAKIDNKKYKAEFNCKAKMLTPEEVLEFTGHAIGGVCPFG
                     LKNSIKVYLDDSMKRFDTVFPACGSSNSAIELTCEEMEKFSKCEKWVDVCKNW"
     gene            complement(11046..11588)
                     /locus_tag="ACGI59_002427"
     CDS             complement(11046..11588)
                     /locus_tag="ACGI59_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9740211.1"
                     /translation="MKKFIKVSFFILGIFTLFIAINKLVYRDMVSVKDYEINHLEEIV
                     FDFDSRRSRDVGTIVRFKISNNSKYSYKLNSAKMKFENCVENKKGMNKCSAYVNVDLF
                     NEKNSITERTLKYGIKPNNQGYVDFVIPKGLNFDYRYFNESGMSVEYKGEYIVNIPMA
                     KGLYLVVGKNNDTWNANIME"
     gene            complement(11640..12326)
                     /locus_tag="ACGI59_002428"
     CDS             complement(11640..12326)
                     /locus_tag="ACGI59_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HFG9740212.1"
                     /translation="MFNLIRKDLIIGISSDGIRNLKYILLFFVFYFFLNSISYYTVSI
                     VVSYLIFINTFECDYENDSRIFIRSMPVSIEDIVYSKYLLGVGLIISVTIIVSLLSKL
                     TSLVFFRNMVLNDVFFSVNIFLAILTILLPLIFKFGYGKMKVCGFIVSILIYFVYGSL
                     LKMISMIVYQVKHFNYSKVGGVYLSNYVTDIANTKYINLYSMTFLTIIIFIISMYFSI
                     KISKKNKFNY"
     gene            complement(12326..13186)
                     /locus_tag="ACGI59_002429"
     CDS             complement(12326..13186)
                     /locus_tag="ACGI59_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9740213.1"
                     /translation="MFSIRNITKKLGKFKLNNINLELKEGDIVGIIGPNGSGKTTLIK
                     IIMGIIDADEGEIELCNETIENSPISFKNNIGFVYDSLQFYPHLKVKEFRKIVSLFYK
                     NFDRERFDEYLNKFDIEENMHIENLSKGQSEKLMLSSALSHNAKLLILDEPTAGIDPI
                     VRTEIMQYLQDFVKNGSSSVIISTHNTDNLIKIADYLVFINRGNQIFTVKKELIEQEY
                     KIIRANKAELEAIKESIVGVKEYKYYNEALVKVGDSLKVKSLLIEIDKHKVKNPTIEE
                     LMYYYVNEVR"
     gene            13321..14121
                     /locus_tag="ACGI59_002430"
     CDS             13321..14121
                     /locus_tag="ACGI59_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HFG9740214.1"
                     /translation="MKRIITINTNQLNKSLETKELLTRKLINAGFEVYSDIYPDTELI
                     ISIGGDGSFLRTVRDFDFPEIPIMGINTGHLGFFPDILPDKIDSFIEAYTKKDYIIQE
                     MSLLNAEVYTTTSGSNMLAVNEVVIRGDKSRTIHLNLSLDNKHIQNFSGDGMIISTST
                     GSTAYNYSAGGSIVDINLELMQITPLHPINTNAYRCFTSSIICSNESVIKIAPEYRFE
                     DSVLIVVDGVEHRFRQIENIKVSISDAKIKLLRMSNYEFWHRVSEKFL"
     gene            14146..14715
                     /gene="yfcE"
                     /locus_tag="ACGI59_002431"
     CDS             14146..14715
                     /gene="yfcE"
                     /locus_tag="ACGI59_002431"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HFG9740215.1"
                     /translation="MKIGIMSDTHGSLLYFEKALNVLSDCDVLLHGGDVLYHGPRNDI
                     PEGYNPKKFIETLNKLENIVIVKGNCDADVDQMVIEHPIQSPYVMSQFGEIRIILNHG
                     YIESEEEIIDKAKKMGGDILVLGHTHVKKLYMDDNLIVINPGSTSIPKDGSHSVAIID
                     IIKTDDEDELELDINLIDINTGNIININD"
     gene            complement(15050..16102)
                     /locus_tag="ACGI59_002432"
     CDS             complement(15050..16102)
                     /locus_tag="ACGI59_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp18"
                     /protein_id="HFG9740216.1"
                     /translation="MKIKSKKIIAFALSLGIMLPSCVYADTISKNQNAQVDMNNYLQG
                     RKSPKEEKLIGEDRFDTAIKISQSGWNNGSERVFLVNSNSLPDALASTPLASKLDAPI
                     LLTNKNSIPYNVTEEIKRLNPAEVILIGSEGAISSSVKESLEDMEVLVSRIGGADREE
                     TSLLLTRQLDDTGDLGVSKVAVVNGYNGLADATSISSPAASDNTAIIYTGKDSIRSEA
                     KNFITQNSTKTYIVGGEYSISKKLEGQLVNSERLAGTDRKDTNAKVLEKFYGKSSKVN
                     NMYFAKDGSGREADLVDGLAAGVLASKTKSPVVLASGSLSSAQKSFIKKVKADKFVQV
                     GGGKNSKPYAEALALQ"
     gene            complement(16309..17535)
                     /gene="pepT"
                     /locus_tag="ACGI59_002433"
     CDS             complement(16309..17535)
                     /gene="pepT"
                     /locus_tag="ACGI59_002433"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFG9740217.1"
                     /translation="MKQKVVERFLKYVSFDTTSNSQCENCPSSEGQRVLAKYIVEELK
                     TMGVDDVSLDENSYIMATLKGNTDGVDTIGFISHLDTIEDVSGKDIKPRIIENYDGKD
                     IVLNEALNVITYVKDSPELEEFKGDDLIVTDGTTLLGSDDKAGIAEIVTAIEYLINHP
                     EIKHGDIKIGFTPDEEIGRGADLFDVEKFGAKYAYTLDGGIVGELECENFNAANATIT
                     IHGRNVHPGSAKNKMVNAIHIAAEISEMFPADERPETTEGYEGFWHLNSIGGNVENVS
                     MAYIIRDHCKEKFENRKSIMIENIEKINKKYDNRVELDLKDSYYNMKEKIEPVMFIVD
                     IAKEAMEELGIKPRLVPVRGGTDGARLSFNGLPCPNIFTGGLNFHGKNECIPVSSMEK
                     ATKLIVRIAEKYAERV"
     gene            complement(17625..18677)
                     /gene="ytvI"
                     /locus_tag="ACGI59_002434"
     CDS             complement(17625..18677)
                     /gene="ytvI"
                     /locus_tag="ACGI59_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
                     /protein_id="HFG9740218.1"
                     /translation="MSILENNFLYKLKNNTIFFCIYTLLFIFIYKAFPYIAPFFLGAI
                     IALMINPISQKLENKFRINKGISTLVLSFLAVAIVSTVTTIIVINSMKELMGFLNNIS
                     ANPEDISNTIMYFLNKINDFMKSFQEIANFDVEQLVNKFSGEVMQITKNLLTSILGLA
                     TSIPYIIIFIITLFIATYFIAKDLDKIENSFYNMFTVDVRKKVKNVKKEAGLSLVGYI
                     RAYTILMAITFFAIWGSFALFGLKYGLIVGFVGALMDLIPFLGIITIYLPVIVYYFLI
                     KNYFIAISMTVIFFVLSLIREILEPKLVSVNVGLNPLATLAAIFIGIQVKGIIGVIFC
                     LGLVCMHDILKKVDIF"
BASE COUNT         6031 a         3400 c         2028 g         7324 t
ORIGIN      
        1 catccttctt ctccaaaaaa tctatctaac ataacaaagc cctccttttt ttaataactg
       61 tgttcttatt ctaagcatcc accacagcag caaagtatta ataaaactat taggaatggt
      121 atccatgcca ttatgtcaaa ttctgcccag ctatctccaa aagctaagaa taggaagaat
      181 agtactatta tccaccatcc tcctccacca aaacatcctt cttctccaaa gaatctatct
      241 aacattatac gttacctcct gttgatattt tgttgagctt ttcgctccta acatatacta
      301 tgcatttggt ttccattttg ctaatgtatt tttactattt ttttaaattt tttaatttga
      361 actaaaaaag gtaaaaccat aacttgtata agttactggt tttacctatt taataatcaa
      421 tattttttag caacaacaac catcatcgaa gttaaataat aacaatacta ttaatataac
      481 ccatatccat atattgtcat cgcataataa gtcttctaaa atgtctgtgc ataatagtaa
      541 gaatactata actattatta tcaacatttt attcccaaaa aatccttcgc atcctccaaa
      601 gaaacgttcc atattgtctt ctaattttgt cataactaat cctccttgaa tcattaagag
      661 actaagtttt gtctctttaa ttaaatatat gtagtaatag acagataagt tacttctaaa
      721 aagtttttat tgattagttt atttatttct ttttcaccaa gtttattgta tctatcaact
      781 tcatccagat aatctttttt ctcctctgat atagtataga ttaaactttc tattgatttt
      841 attttgtact ccttatatct ttctatcaag aaggatgttt ttgaattttc tattcctttc
      901 tttacatgca ttaaccttga tatgtagtct tctttttcat tagatacata actctccttt
      961 tcagctacat cttcaaggat taaattggtc tccattacat aatcctctat acgttgcata
     1021 tctaaagtta ttgaattata ataaaaattt actgctataa gtaaaaacat tcctactgaa
     1081 gttagtaata gtcctacata tttcatagca tttcctccta agttatattt gttatcaata
     1141 aagtttattc atcaaataat actttattac tacatttata aaattttatt attttttata
     1201 gattccatat tgaaaaattc ttgatattat attaaactaa cattaaaatt aattaaaact
     1261 gctttaggag gcttaaaatg tttgagaaaa taaaagaaat aatagcagaa caattagggg
     1321 tagacaattt agatgagata actatggaag catctttaat ggacgactta gaagctgatt
     1381 cattagatgc tgttgaaatt ataatggccc ttgaagatga atttggaata gaaataccag
     1441 acgaggaagc tgagaacttt aaatgtattg gtgatatatg taaatatatc gaagaaaaca
     1501 aatagtatcg ttttgtctac aaatataaat agtctgtgta aacttctaca taataaatta
     1561 tattagagtt cgcacagact agattacatt tatttacaat atataaaata cacgctctta
     1621 tatttaattt atatactaag tatataaatt acttttcttc ataaaacgta acattcttta
     1681 ttattatatc tatagttcca tcttgatttt gttttacagt atacttcatt gggtcttcaa
     1741 aatcagttag attccctttt atgtcaaaac cattatccgt ttttatactt ctttttttaa
     1801 gctttttctc aacccatttt ttatctatac taaaactttc gtcaagacct ttttcttcca
     1861 tatgctcttt aaagctatcc tttaactcat catctttaat tgaattatca acaaagtcat
     1921 ttatatcaac ttcatgtttt tccttcagtg tataatttaa tatacttctt acatcttcag
     1981 cttgctttat atcattacta agagcattgg ttatccaatt ttcagctgta tttttaaata
     2041 ccttggtctt gtacttatca tcatctattt ttttagcatt taaaaattct gttacaaact
     2101 tagattctga cccttctttt tcagcatcct tatctaaaag ccttagatga aactcatcat
     2161 ttataccact tagactaatc aaagctcctt gcttttgtct taaagtctct gggataccaa
     2221 tttcatttga aaccatttga atattaaatt tatcatccac aaattcaatt gaatgagtgt
     2281 acaacttttt ataatctaat ttcagtattg ctacactctt ttcatcttta atagtgtata
     2341 aacaaattgc caaatcacaa gattctagtg cagcattaat tttcataacg tcaaacaagt
     2401 aagatgcaat ttctttagaa tttttcaaaa aagtactttc atcatatatt atttgttcac
     2461 aacagttttt aattaaattg tcattataat ctttaaaaac accttttctt aaatcctcat
     2521 cttttgcaat tctttttata gctttctgga aaaaaccatc cacttcttga ttaactttgc
     2581 cttcaaagtc atttaatatt ggaacatcac tatttttgtc caaaacatgt attataaatt
     2641 tatgtattat catatatatc accccattat cttaatttcg atattaactc tttcatatcc
     2701 tctggtatat ctgctttaaa tttcaatttt tcttttgttc ttggttgttc aaactctagc
     2761 ttatatgcat gaagtgcttg cctatttatt agctcttcat ctacataacc atacaactca
     2821 tcacctatta ttccatgacc tatatgagcc atatgtactc ttatttgatg tgttctacca
     2881 gtttctaagc ttacttctaa tacagttgca ttttctagtc tttctattac tttatagtga
     2941 gtaacagatg actgtcctct ttcatctatt atcctcttta ttgaatcttc tgtaggtcta
     3001 taaattggct catctatagt tccctcattt tcttttataa ttccatcaac tactgttata
     3061 tacttttttt caactttatt ttcactcatt gctgtagaca aagtatgatg tgcataagca
     3121 ttttttgcta ctataactaa ccctgaagta ttcatatcta acctatttac aaatcttatt
     3181 tttacgtttt ctttttgatt atctatatag taactaatac catttgctat tgttttatca
     3241 tagtggcttt ttgttgggtg aactaccata aacggaggtt tattcaccat aattatatca
     3301 aaatcatcat aaattatctg taaatttaaa tcttgaggtt caaaatttgc tttttcctcg
     3361 tctattttta cttctattaa atcaccacta tacaccttta aactcggttt tttatatact
     3421 ccatttacta aaacactctt ttccctcttc atttttgata atgacctaac tgagaaattt
     3481 agcttgtcca ataaaacttc ttttaaagtc atttcttctt catttgtata cgatataaga
     3541 ttatatcttt gattctcttt tttaaacaaa cttttaccta ctttctttta tatcttccta
     3601 ctttttatat cttcaaaatc atgatattat atattttaca ctatttttgt ttaatagata
     3661 ataattattt ttaactataa aaacctgtaa gaataacaaa tagttattct tacaggttaa
     3721 attcataatt aaatatttaa tttgtaaata ttataatcta aaactatctc ttaactatta
     3781 aagtagttcc cattcctcca cctatacaaa gtgtagcaag accagtttta gcatctcttc
     3841 tcttcatttc atataaaagt gtagtaagta ttcttgctcc tgagcatcct attggatgtc
     3901 ctatagctat tgctccacca ttaacattaa ctttattcat atctatattt aagtctctta
     3961 ttacagctac agattgggca gcaaatgcct cattagcttc aactaaatct atatcttcaa
     4021 tagtcatatt agcagcttct aaagcttttt tagttgctgg aactggtcca tatcccatta
     4081 ttttagggtc aacaccagct gttccataag aaactatagt tgcaagaggc tctattccta
     4141 gttcttcagc tttttcttta gccattacta ctagcatagc agcaccatca tttattcctg
     4201 atgcattacc agcagtaact gttccatctt ttttaaatgc aggtcttaac ttagcaagtt
     4261 tctccattgt agtgccaggc ttaatatatt catctttatc tactacagtg tcaccttttc
     4321 ttccttttat aacaacagga actatttctt catcaaattt tccttcagct tgagcttttt
     4381 cagctttatt ttgacttgca agagctaatt catcttgttc ttctctagtt atattccatt
     4441 gctctgctat gttttcagca gtaataccca tgtgatagtt attaaatatg tctgataatc
     4501 catcttttat cattgaatca acaaaagcag catcacccat tcttgcacca tatctcgcac
     4561 ttggtactaa ataaggagac atactcatgt tttcagctcc accaactaac attatatcag
     4621 catcacctaa tgctataagt tgagatgcca ttgaaacaga tcttaatcca gaaccacaaa
     4681 ctatatttat agtcatagct ggtttttcta ctggtattcc tgctcctaat gctatttgtc
     4741 ttgctatatt ttgtccaaga cctgctgtaa gtactccccc taaaagagat tcatctatca
     4801 tatctggagt tatgttagct ctttttatag cttctttagc tgctgttacc cctaactcta
     4861 ccgctgaaac tgatttaaat gctcctccaa aacttcctac tgctgttcta gctgcactgg
     4921 caattactac ttctctcata ttcattaccc ccattaaatt agatttttca tatatccgaa
     4981 attgtttttt aaacaatttt atttaaactt ttaattccca aattcttatt tattataatc
     5041 atagaatcct atcttagttt ttcttcctaa ttgattagct ctaaccattt tagctaaaag
     5101 tggatgaggt ctatatttag tatctccaaa ttcagtatat aaaacgttca ttatagctaa
     5161 aacaacatct aatccgatta aatcacctaa tgctagtggt cccattggat ggtttgctcc
     5221 taatttcata gcttcatcta tttcttcttt acttgcaaca ccatctgcat atataccaac
     5281 agcttcattt atcataggta taagtattct atttactaca aatccaggag attcagatac
     5341 atctactggt actttattga tactcttaga taattcaaat actgtatcaa aagtaacttt
     5401 tgatgttaac tgaccactta taacttcaac taatttcatc ataggaactg gattaaagaa
     5461 atgcattcct ataactttat ctggtctctt agtagaagaa gctatttctg ttatagataa
     5521 tgatgaagta tttgttgcca agatagtatc ttctttacat aattcatcta gtaacttgaa
     5581 aacatctttc tttatattca tgtcttctac agatgcttct attattaaat ccatatcttt
     5641 taaatcttca tagttagtag ttgaactaac atgacttaat atttctgctt ttgtagcttc
     5701 atccattttt cccttagtaa ctaacttagt taaattttta tctaataaag ctaaacattt
     5761 atctatagca ccttgagttc tactctttaa acatacatca tgtccacaac ttgcaaaagt
     5821 ttgtacaata ccacttccca tagttccact acctattaca gctaatttca tagttataac
     5881 ctcctgttat tttagtattt atattctgtt aactagatta aaatactatt taatctagtt
     5941 aacagcaaat atatttttaa ttagattttt acccttttat aaagttagct tctctctttt
     6001 caacgaaagc tgacattcct tctttttggt ctttagttga aaaacaaaga ccaaataaat
     6061 tagattctat atctattcca gtatttatat cagtttgagc accaagttgt attgcttctt
     6121 tagagtatct aactgcaagc tgagcatttt tagctattat cttagctaat ttctcaactt
     6181 cttctattaa aatgtctggc tcaacgactc tatttactag ccctattttt tcagcttcat
     6241 cagcttttat aacttgacct gtaaagatta attcttttgc ttttgccatt ccaaccaatc
     6301 ttgtaagtct ttgagttcct ccatatcctg gagttattcc aagagttact tctggctgac
     6361 caaatttagc tttagcagat gcaattctta tatcacatgc cattgcaagt tcacatcctc
     6421 cacctaaagc aaatccgttt acagcagcta tcactacttt tttactattt tctatttctc
     6481 caaaagcttt tgctcctaag atactaaaat ctttagcagc tacagcatct aaatctttca
     6541 tgtatgcaat atctgctcca gctacaaatg cctttccttc ccctgtcaat attacaacat
     6601 caatagtttc atcattatta atatctacaa atacttcata aagttcttct aaagtctttg
     6661 aatttattgc attaagggct ttaggtctat tcattttcac tgtacatata tttccatcta
     6721 cttcaacagc tacattctca taaactttaa catcacttgt actcataata ccctccccaa
     6781 gaaattagtt agctaaaact tcaccttttt cttttgcaac acttaactga ctgataagtt
     6841 ctggaagcac tttatgaaca tctccaacta taccaacatc agcatattta aatattggag
     6901 cttctggatt tttatttata gcaactataa actcagcatc ttccatacca gctatatgtt
     6961 gtattgctcc agatatacca catgctatat aaaggtctgg tcttacagtt ttaccagttt
     7021 gaccaacttg tcttgcttta tctaaccaac ctgcatctat agtggcacga gaaccagaaa
     7081 cttctccacc tataatttca gctaattcat aaagtatgtc taagttttct tttccaccca
     7141 ttccacgtcc agcagaaact aatatcttag catcttctat tttaacttgt tttttagctt
     7201 cttttattac ttgtacaact tgaactaatt tatcagcatc attaaattct accttgaaac
     7261 ggttaattac agcttcttta gtttcatcag gttcattttt cttcataacc cctggtctaa
     7321 ctgtagacat ttgaggtctg aaatctttac aaactattgt tgccattata tttccaccaa
     7381 aggcaggtct tgtcattaat aataattttg tatcttcagc tactgcaaga cctgtacagt
     7441 cagcagtaag acctgtatgt attctagcag aaactctagg tgctaaatct ctacctattg
     7501 aagttgcacc aaataataca actatagggt cagctgcttt tattgcttca taagctgctt
     7561 ttgtatatgg ttcagttgta tacactgcta aagcttcatc atctactact attacctcat
     7621 ctgcaccata gtgtgctaat gtatctatta aaccttctac cttactacct aaaagtaatg
     7681 cagaaacttt tgtatcataa tcttttgcta tttctgtagc ctttcctagt aattctaaag
     7741 aaacagtttg aattacattt tctctttgtt ctattactac taaaacgtta cccattcttt
     7801 atcccccctt aaattatatg atatacttct cttttaattt atctatgata attccagctg
     7861 atgttttcgc atcttcattg tatattgtac cagctggttt aactgatttt gtaaatgatt
     7921 taaatacact agttggagaa cctttaagac ctaaattaga agggtcaact tctatatctt
     7981 ttacagtcca tgtttctact acatcatttt cgaaagcatc atatattctt ccaactttca
     8041 tgtatcttgg tgtgttcata tctttaagag ttgttataag gcatggcatt ttaactttta
     8101 agtcatggca acaatcttca aattgtcttt ttactaatac atattcacct tcagttttta
     8161 tttcttcagc atatgttatt gatggaagat ttaaatgttc agctatttga ggtccaactt
     8221 gtgcagtatc tccatctatc gcctgtcttc cagctattat aatatcaaaa tctatatttt
     8281 ttaatgctcc tgctaatgct gatgaagttg cccaagtatc agcacctgca aatgctctat
     8341 ctgttaataa tatacctcta tctgcaccca ttgctaaagc ttcttttaaa gccatatctg
     8401 cttgaggagg tcccattgtt ataacagtta catgagcacc catttcttct tttaatttta
     8461 tagcttcttc taaacctgct ttatcatcag ggtttattat acttggtact ccatctctaa
     8521 ttaaagtacc tgtattagga tctagtttaa cttctgttgt atctggaact tgttttatac
     8581 aaacgactat attcataaat ccttcctcca tatctatttt aatatcgacc tattgtgtat
     8641 attttaatat ttatctattt taataatttt cctgaaataa ccattctttg aacttcacta
     8701 gttccttcat atatttcagt tatcttagca tctctcatca ttctttctac tggatagtca
     8761 cgagtgtatc catatcctcc atgaagttgt acagcttttg tagtaacttc catagctgtt
     8821 tcagctgcaa ataattttgc cattgctgct tctactccat aaggttttcc taagtcttta
     8881 tttatagctg cttgatatac aaggtgtcta gccgcttgta ccttaacttc catatcagct
     8941 aattggaatt gtgtattttg gaattttgat aatggtctac caaattgtac tctttctttt
     9001 acatatttaa cagtttcatc aagagcacct tgtgctaaac ctaaagcttg tgcagctata
     9061 ccaattctac caccatcaag agtagacatt gctatcttaa atccttgacc ttcttttcca
     9121 agtaaatttt ctttaggtat tctgcaatcc tcaaatatta attcactcgt agctgaacct
     9181 cttataccca ttttcttttc tttaactcca aagctaaacc caggagttcc tttttcaact
     9241 ataaatgctg atattccttt gttcccctta gatttatcag tcattgccat tactacatat
     9301 atgtcaccag ctattgcatt tgttataaat atttttgagc cattaagtat gtattcatcc
     9361 ccgtctaaaa cagcagttgt ttgttggcca gacgcatctg taccagcatt aggctcagta
     9421 agaccaaatg ctcctaattt ttctccactt gctaatggtc ttaagaattt ttgtttttgt
     9481 tcttcattac catattgata tataggccat gagccaagag atgtatgagc tgataatata
     9541 actcctgtag taccacaaac tctagacaat tcttcaactg ccattatata tcctacagtg
     9601 tctccacctt ctccaccata ttcttttgga tatggtatac ccatcattcc tgcttttgcc
     9661 attttttcca ctgtttcata aggaaatctt tcttcttcat caagttctgt tgctaaaggt
     9721 ttaacttcat tttcagcgaa gcttacatat agctctttaa gcatctgata ttttttagaa
     9781 tttaaatcca taatagcctc ctcagtattt tataaatatt ttattttcat ttattaatta
     9841 aaatctcggc aacatctata ataaggtatt tgaatatacg tcgagaaatt tataacctta
     9901 atcaaactac gctctatatt tatatgtgat tatactaacc aattatgaca ttaatttaat
     9961 cataaattgt taatttaaaa tcaactatac ttaaaaaaaa tctcgctttt tggaattttg
    10021 tgaaatttca agttagaata agatttcttt cacactggtt agagataaaa taatttgttt
    10081 acgatattta tattaaggta tattaaacat taaatattgt ctgtacatct gttttttagc
    10141 catgtacaga agatttttat cgtataaaaa ttattttaat agagctaaat aatcatattt
    10201 ttatctgact attttttatt tttaaatatc ttacatctat aatagtatca ctcaaaggat
    10261 aatttttcaa taatttctaa ttattttttt aacaatattt attacatttt ttccactttt
    10321 ttgtcatttt tttattgttt cgaaaaaaaa tcagaatttt ttaactttat tttggattat
    10381 tagtttaaat tagattttcc tcttagattt ataaatattt acacaaaaaa actacctctg
    10441 tatagattgt atacagaggt agttttttac taattcctac tatagtttac tatatttttt
    10501 taccaattct tacaaacatc tacccatttt tcacacttag aaaacttctc catttcttca
    10561 catgtaagtt ctattgcaga gttactactg ccacatgcag gaaatactgt atcaaatctt
    10621 ttcattgaat catctaaata gacttttata gaatttttta aaccaaatgg acagactcca
    10681 ccaatagcat gtccagtaaa ctccaagact tcttctggtg tcaacatttt agccttacaa
    10741 ttaaattcag ccttatactt cttattatca atcttagcat caccagctgt cacaattaaa
    10801 attgcatcat ctcctatttt aaatgagagt gtcttagcta ttcttgctgg aataacccca
    10861 gctgcttcag ctgcaagttc cacagtagca ctagattgtt caaattctaa tatactgtct
    10921 tccttgccaa attgtttaaa atactctctt acattactta ttgacattaa atacccctcc
    10981 tagtctgaac ttttataaat ttcatattat tcttaattat atctataatt gatttatttt
    11041 tcaaattatt ccataatatt agcattccat gtatcattat tttttccaac tactagataa
    11101 agtccttttg ccattggaat attgacaata tattctcctt tatactctac actcatacca
    11161 gattcattaa agtacctata atcaaaattt agtccttttg gaattacaaa atcaacataa
    11221 ccttgattat ttggttttat tccatatttc agagttcttt cagttataga atttttttca
    11281 ttaaataaat ctacatttac atatgcagaa cacttattca ttcccttttt attttccaca
    11341 caattttcaa acttcatttt agcactgttt aatttataac tatatttact attattgctt
    11401 attttaaatc ttacaatagt accaacatct ctacttctcc tgctatcaaa atcaaaaaca
    11461 atttcctcta aatgatttat ttcataatct ttgacactta ccatatctct atatactaat
    11521 ttatttatag ctatgaatag tgtaaaaatc cccaaaataa aaaatgaaac ttttataaac
    11581 ttcttcattt ttagtctcct tatattttgt gctattgtgc tataatatta atcaaaattt
    11641 taataattaa acttattctt ttttgaaatc ttaatagaaa aatacattga tattataaaa
    11701 attattattg ttaaaaatgt catactatat aaatttatat acttagtatt agctatatca
    11761 gttacataat ttgaaagata tactcctcca actttactat aattaaaatg ctttacttga
    11821 tatacaatca tactaatcat cttcaaaaga cttccatata caaagtaaat taaaatactt
    11881 actataaaac cacaaacttt catttttcca tatccaaact taaaaattaa aggcagtaaa
    11941 atcgtcaaaa ttgctaaaaa tatatttaca gaaaaaaata catcatttaa aaccatattt
    12001 ctaaagaaca ctagtgatgt caatttactt agtaaggaca caattattgt tacagaaatt
    12061 attaatccta cacctaataa atattttgag taaactatat cttctatact aactggcata
    12121 ctccttataa aaatccttga atcattttca taatcacact caaatgtatt tataaatatc
    12181 aaataactaa caacaatact tactgtataa tatgaaattg aattaaggaa aaaataaaac
    12241 acaaaaaata gcaatatgta ctttaaatta cggataccat ctgaactaat ccctattatt
    12301 aaatcttttc ttattaaatt gaacatcatc tcacctcatt tacataataa tacattagtt
    12361 cttctatagt aggatttttg accttatgct tatcaatttc tatcaatagg ctttttactt
    12421 tcaagctatc tccaacttta acaagtgcct cattataata tttatactct ttcacaccaa
    12481 ctatgctttc tttaattgct tctaactctg ctttatttgc cctaattatc ttatattctt
    12541 gttctattaa ttctttctta acagtaaaaa tctgatttcc tctatttata aaaacaagat
    12601 aatcagcaat tttaattaaa ttatcagtat tatgagtaga aattattaca ctactacttc
    12661 catttttcac aaaatcttgt aagtactgca ttatttcagt tcttacaatt gggtctatcc
    12721 cagcagttgg ttcatccaaa atcaataact tagcattgtg actaagtgca cttgataaca
    12781 taagcttttc agattgacct ttagataaat tttcaatatg catattttct tctatatcaa
    12841 acttattaag gtattcatca aacctttctc tatcaaaatt cttataaaat aaagaaacta
    12901 tttttctaaa ttccttaacc tttaaatgcg gataaaattg taaagaatca tatacaaaac
    12961 caatattgtt tttaaagctt attggagaat tttcaatagt ttcattacaa agttcaattt
    13021 caccttcatc tgcatcaata atccccatta taatttttat taaagtcgtt tttcccgaac
    13081 catttggtcc tataatccca acaatatccc cttcctttaa ctctaagttt atattattta
    13141 acttgaattt tcctaatttt tttgtgatat tccttatgct aaacacatct tcacctctta
    13201 tgcatttttt tatacccacc tatacatatt ttaacacatt cactacaaag gtcatttata
    13261 aatttataaa ttaagaatta taaaactatg ttataataca aagtatgaag aggtgaatat
    13321 atgaaaagaa ttataactat aaatacaaac caattaaata agtcccttga gactaaagaa
    13381 ctcttaacta gaaaacttat caatgctggc tttgaagtgt attcagatat ttatccagat
    13441 acagagctta ttatatcaat tggtggtgat ggctcttttt taagaacagt tagagatttc
    13501 gattttcctg aaattcccat aatgggaata aatacaggtc atctggggtt ttttcctgat
    13561 attttaccag ataagattga tagctttatt gaagcttata caaaaaaaga ttatattatt
    13621 caagaaatgt cacttcttaa tgcggaagta tatacaacta ctagtggttc taatatgctt
    13681 gctgtaaatg aggttgttat aagaggagac aagtctagga ctatacacct aaatttaagc
    13741 cttgataata aacatataca aaattttagt ggagatggta tgattatttc tacttccaca
    13801 ggttcaacag cctataacta ttctgctgga ggtagtattg ttgatataaa tcttgaactt
    13861 atgcagatta cacctttaca tccgataaat accaatgcat ataggtgctt tacatcaagt
    13921 ataatatgtt caaatgaatc tgttataaag attgcaccag aatatagatt tgaagattct
    13981 gtattgattg tcgttgatgg agttgagcac agatttagac aaatcgaaaa tataaaagtt
    14041 agtatatctg atgcaaaaat aaaattactt agaatgtcaa actatgagtt ttggcataga
    14101 gtatctgaaa aatttttata gactgtactt aggaggattt ttaatatgaa aataggaata
    14161 atgagtgata ctcatggtag cttactttac tttgaaaaag cgttaaatgt attatctgat
    14221 tgtgatgttt tattacatgg tggagatgtc ttatatcatg gacctagaaa tgatattcca
    14281 gaaggatata atcctaaaaa atttattgag acattaaata aattagaaaa cattgtgatt
    14341 gtaaaaggga actgtgatgc agatgttgac cagatggtta tagaacatcc tattcagagt
    14401 ccttatgtca tgagccaatt tggtgaaatt agaattatat tgaatcatgg ttatatagaa
    14461 tcagaagaag aaattattga caaagcaaag aaaatgggtg gtgacatatt agttttagga
    14521 catactcatg taaaaaagct atacatggat gataatttaa tagtaataaa tccaggaagt
    14581 acttctatcc caaaagatgg aagccattct gtcgcaatta tagatatcat aaaaactgat
    14641 gatgaggatg agcttgaact tgatataaac ttaattgaca tcaatactgg aaatataata
    14701 aatatcaatg attagtaaat ttttaataaa aataaaataa gctttaattt attaaactca
    14761 taataaaaac aagtaaacca ttttatttct atctcaatta gaagtttaag tacaatacct
    14821 taaacttcta attaaataga atctggttta cttgttttat tatatgctat attgaaatat
    14881 aacttagttt acttatttca ttatatatta tactgaaata taacttagtt tacttatttt
    14941 attatatatt atattgaaac atagcttagt ttacttattt tattatatat attgaagtgc
    15001 aacttggttt acttgctatt attatatatt aattttgatt tacacaatat tactgtaagg
    15061 ctaatgcttc tgcatatggt ttactatttt tacctccacc aacttgtaca aatttatcag
    15121 cctttacttt ttttataaaa cttttctgtg cactacttaa acttccactt gcaagtacta
    15181 caggtgactt tgtttttgat gctaatactc cagcagcaag cccatcaact aaatcagctt
    15241 ctctaccact tccatctttt gcaaaataca tattattaac cttacttgat tttccataaa
    15301 acttttctaa aactttcgca ttagtatctt ttctatctgt ccctgctagt ctttctgagt
    15361 ttactaactg tccttcaagt tttttactta tactgtactc cccacctact atgtatgtct
    15421 tagttgagtt ttgtgtaata aaattctttg cctcacttct tatgctatcc ttacctgtat
    15481 atattatagc tgtattatca cttgcagctg ggcttgatat tgaagttgca tctgccagtc
    15541 cattataccc attaaccact gccacttttg agacacctaa atcaccagta tcatctagct
    15601 gtctagtaag aagtaaagat gtttcttctc tatctgctcc accgattcta ctgactaata
    15661 cttccatatc ttcaagagat tcttttacac ttgatgaaat agctccttca cttccaatta
    15721 aaataacttc tgctggatta agccttttta tttcctctgt tacattatat ggtatactat
    15781 ttttatttgt aagcagtatt ggtgcatcca atttacttgc caaaggagtt gaagccaatg
    15841 catctggcaa tgaatttgag ttaactagaa acaccctttc tgaaccatta ttccaaccac
    15901 tttgagatat ttttatcgct gtatcaaatc tatcttcacc aataagtttt tcctcctttg
    15961 gagattttct accttgaaga taattgttca tatctacctg tgcattttga tttttactaa
    16021 tagtgtctgc atatacacaa cttggcaaca taatcccaag tgaaagtgca aatgctatta
    16081 tttttttaga tttaattttc aatacaattc ccccttttaa atgttaatac ctattgatat
    16141 tcatactttt tattgatacc ctaaactaaa aataattatc ttttataaaa ataattacaa
    16201 aaatagctat accattttaa gtacaacaca agcttataat cagcttgact tataaagaat
    16261 gatatagcta ttataacttt aaatgcttaa tttttaaata ctttttaact aaactctttc
    16321 agcatatttt tcagctattc ttacaattag tttagtagct ttctccatag aactaactgg
    16381 tatacactca tttttcccat ggaagtttaa tccaccagta aatatatttg gacaaggtaa
    16441 tccattaaaa gataatcttg ctccatcagt accacctctt acaggaacta aacgtggttt
    16501 tatacctaat tcctccatag cttctttagc tatatcaact ataaacataa ctggctcaat
    16561 cttttctttc atattgtaat aagagtcttt taaatccaat tccactctat tatcgtattt
    16621 cttatttatt ttttctatat tctctatcat aatagatttt ctattttcaa atttttcttt
    16681 acaatggtct ctaataatat aagccatact tacattctcg acatttccac ctatagaatt
    16741 taaatgccaa aatccttcat atccttctgt tgtttctggt ctttcatctg ctgggaacat
    16801 ttctgatatt tcagcagcta tatgtatagc atttaccatt ttatttttag ctgaacctgg
    16861 atgaacattt ctaccatgta tagtaatggt tgcatttgca gcattaaaat tttcacattc
    16921 aagttctcca actattcctc catctaaagt atatgcatat tttgctccaa atttttcaac
    16981 atcaaataaa tcagcacctc taccaatttc ttcatctggt gtaaatccta tttttatatc
    17041 tccatgctta atttctggat gatttattag atactctatt gcagtcacta tctcagctat
    17101 accagcttta tcatcagacc caagtagagt tgttccatca gtgactatta aatcgtcacc
    17161 tttaaattct tccaactctg gagaatcctt tacatacgta ataacattta acgcttcatt
    17221 taaaactatg tcttttccat cataattttc tattattctt ggtttaatat cttttccact
    17281 aacatcttca attgtatcta aatgtgatat gaatcctata gtatccacac catcagtatt
    17341 tccttttaaa gtagccatta tataactatt ttcatctaaa ctcacatcat caacacccat
    17401 tgtttttagc tcttcaacta tatacttagc taaaactctt tgaccttctg aagatggaca
    17461 gttctcacac tgagaattag atgttgtatc aaatgataca tattttaaaa atctttccac
    17521 tactttttgt ttcacgtata taccctcttt cttaataaga ttaatatttt tttatttacc
    17581 tgtgaataaa attttatttt taaaaaatct atcaattatt ataattaaaa aatatccacc
    17641 ttcttaagta tatcatgcat acataccaaa cccaaacaaa aaattactcc aattattcct
    17701 tttacttgga ttccaataaa aatcgctgct agggtagcta atggatttag tccaacattt
    17761 actgatacta gtttaggttc aagtatttct ctaatcaaag acaaaacaaa aaatatcaca
    17821 gtcatactta tagctataaa ataatttttt ataaggaaat aataaacaat tactggaaga
    17881 tatattgtta ttatacctag gaatggaatt aaatccatta aagctcctac aaaccctact
    17941 attagaccgt atttcaatcc aaataaagca aaacttcccc agattgcaaa aaatgttata
    18001 gccataagta ttgtataggc ccttatatat ccaacaagtg aaagccctgc ctctttcttt
    18061 acgtttttta ccttctttct aacatcaaca gtaaacatat tataaaaact attttcaatc
    18121 ttatcaaggt cttttgctat aaagtacgta gcaataaaca aggtaattat aaatattata
    18181 atatatggaa tagaagtagc caatccaagt atactagtaa gaagattttt tgtaatttgc
    18241 attacttcac cactgaattt gtttactaat tgttctacat caaaattggc tatttcttga
    18301 aatgatttca taaagtcatt tattttattt aaaaaataca taatagtatt acttatatcc
    18361 tcaggattag cagatatatt atttaaaaat cccattaatt ctttcattga atttattact
    18421 attattgtag taactgtact gactatagcc actgcaagaa aacttagaac aagagtagag
    18481 atacctttat ttatacgaaa cttattttct aacttttgtg atataggatt aatcataagt
    18541 gctattatag cacctagaaa aaatggagct atgtatggaa acgctttgta aataaaaata
    18601 aataacaatg tatatataca aaaaaagata gtgttgtttt ttaatttgta taaaaaatta
    18661 ttttctagta tgctcatctt aaaaatctcc tttcttctaa tttatatttt tattttgtcc
    18721 atttcatact agttttattt gaatacaaaa taaaaagaag tgtataaata taaaagatat
    18781 ctt
//
LOCUS       DAYAPA010000076        18740 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.076, whole genome shotgun sequence.
ACCESSION   DAYAPA010000076 DAYAPA010000000
VERSION     DAYAPA010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18740
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.076"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            <1..570
                     /locus_tag="ACGI59_002435"
     CDS             <1..570
                     /locus_tag="ACGI59_002435"
                     /inference="COORDINATES: protein motif:HMM:NF040238.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5906 domain-containing protein"
                     /protein_id="HFG9740219.1"
                     /translation="IFKKLVSGDRVNVERKGQNPFDFNNYSKFLFSANNIPRIKDKTG
                     AVLDRLIIIPFNASFSVNDKDFDPYIKYKLREQEAIEYLINLGLEGLKRVLKNRKFTV
                     PDKVKKEILEYEEVNNPILGFFKEVDKIENESTKEIYMKYQEYCILNGLQPISNVEFS
                     RQVVKKFGYEIKDKKINGKKYRVFINGSR"
     gene            577..714
                     /locus_tag="ACGI59_002436"
     CDS             577..714
                     /locus_tag="ACGI59_002436"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9740220.1"
                     /translation="MVQDIGSRCNAVISMGFKMVQDVFDLYIYLSKKSSQKVDDFAPF
                     I"
     gene            948..1355
                     /locus_tag="ACGI59_002437"
     CDS             948..1355
                     /locus_tag="ACGI59_002437"
                     /inference="COORDINATES: protein motif:HMM:TIGR01636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HFG9740221.1"
                     /translation="MTDYEKLEELLNSYSNLDIEIREIELKVRGSSLRGIELNGMPKG
                     NNVSSPIENELIYVERLENEKIYLQIKKESVNNMLNLLDDFERNLIELRYFKKLQYKQ
                     ISYELNISELYVGKKRKKALDKILPFAKKYNLI"
     gene            1552..2007
                     /locus_tag="ACGI59_002438"
     CDS             1552..2007
                     /locus_tag="ACGI59_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002844934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFG9740222.1"
                     /translation="MAKYEYWITEEGLIKIEGWARDGLTDEQIAFNIGINVKTLYDWK
                     KKYSNICNALKKGKEVIDRQVENALLKRALGYEYDEITYEEGQETKRVTKQVVPDTTA
                     QIFWLKNRKPAEWRDKQIVESTNEITINNPFKELSTEELKRLAKLDDDG"
     gene            1994..3406
                     /gene="terL"
                     /locus_tag="ACGI59_002439"
     CDS             1994..3406
                     /gene="terL"
                     /locus_tag="ACGI59_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HFG9740223.1"
                     /translation="MTMDKKLIQLEAKKELARREFFYFCNLLAPKFYKEDRKYLVETC
                     NKLQDFYYSDDEVLIINMPPRHGKSRSASLFVEWILGKNKNEKIMTGSYNETLSTMFS
                     KNVRNAIQEEKADIDTIIYSDIFPNTKIKHGDGAMNLWSLEGGYNNYLATSPSGTATG
                     FGCSLMIVDDLVKNAEEAYNENVLEKHWDWFTNTMLSRLEEKGKIIIIMTRWSSKDLA
                     GRVLEHYKEEGKKVRHINMKALQEDGNMLCEEVLSLKSYKSKVRAMGEDIASANYQQE
                     PIDLKGCLYTKFKTYEKLPVDGEGNLLFTSIKAYVDTADEGADYLCSIVYGVYNKEAY
                     ILDVLYTKESMEITEYKTAKMFYNNEVNKADIESNSGGRAFARNVQRILKEKFKSNKT
                     IIKWFHQSKNKNARILSNSSWVMEHIYFPINWRDKWIEFYKAMVSYQREGKNKHDDAP
                     DALTGVAEKALKGQGLSVFK"
     gene            3435..4790
                     /locus_tag="ACGI59_002440"
     CDS             3435..4790
                     /locus_tag="ACGI59_002440"
                     /inference="COORDINATES: protein motif:HMM:NF016988.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0046798 - viral portal complex [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0051027 - DNA transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFG9740224.1"
                     /translation="MELEKIRAIISADIARRQEILQAKSYYYNENDILKKGVVVQNRD
                     ENPLRNADNRISHNFHEILVDEKASYMFTYPVLFDIDNDKELNEKVTDVLGNEFTRKA
                     KNLAIEASNCGTAWLHYWIDEEYSGEQVTNQTFKYGVVNTEEIIPIYRNGIERELEAV
                     IRYYVQLEDVKGQIQKQAYTYVEFWTDKILDKYKFFGVTCCGSQIEHITVQHRFNSVP
                     FIEFANNIKKQSDLSKYKSVLDLYDKIMSGFANDLEDIQQIIYILENYGGEDTAQFLN
                     ELKRYKAIKTETDSEGDSGGLKTMQIEIPVEARKVILEILKKQIYESGQGLQQDTESF
                     GNASGVALKFFYRKLELKSGLLETEFRTSFDKLIKAILYFLGVTDYKKIQQTYTRNMM
                     SNDLEDADIATKSVGIIPTKIILRHHPWVDDPEEAALLLREEKKIQASKVSDDYNNFT
                     E"
     gene            4803..6221
                     /locus_tag="ACGI59_002441"
     CDS             4803..6221
                     /locus_tag="ACGI59_002441"
                     /inference="COORDINATES: protein motif:HMM:NF016148.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin type 50 domain-containing
                     protein"
                     /protein_id="HFG9740225.1"
                     /translation="MNNNIEYWKEREKQRLNSRLKEEKEVLKELDKQYKIAMKNIEKE
                     IANLFYKYAEQNKLTYAETQKYLTNNEFNTWRMDIKQYIKLIEQTSDERLLLELNTLA
                     MKSRINRLEELFYQISKEINNTFDIQNNRVEKLLEESVKDSYYKSIYETQKHVGVGVS
                     FTKLDREALKDIITYPWSGKNFSQRIWKNRDLLSEVIKEEITQMVIRGESLKKISNRV
                     SEKMDSSYENAIRLVQTEHSHFMSEADKKAYESQKVDKYQFLATLQDNTCKRCRSVDM
                     KVYLVKEAKEGENYPPLHPRCRCTTIPYFEDEKGGTRTARLLKGKTYEVPFNLSYNEW
                     YKNHVEVAINREKENKNNVIINNIKKDIKDGKYNLNIHNGKQGKHFKDHNNYTEGRSY
                     LTVSKEEVQELINKYAGTGILELDRNGKFKNKELITCEKNIGVNISNLTGEETETNKF
                     YIHYSKNGTHIVPTMKGVEKDE"
     gene            6214..6411
                     /locus_tag="ACGI59_002442"
     CDS             6214..6411
                     /locus_tag="ACGI59_002442"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9740226.1"
                     /translation="MSKNLINYMSEKVKIIDIDNKEWIGYVKTYTPAIDSDNEINEIG
                     LKQEDCLISFQEDEIKSIEII"
     gene            6479..7321
                     /locus_tag="ACGI59_002443"
     CDS             6479..7321
                     /locus_tag="ACGI59_002443"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9740227.1"
                     /translation="MAKFKKKPVEVEAFRLGYDIEPEWFFENSRVCNFIQEKCIDGNV
                     SCDLKTLEGTMRANKGDYIIQGVKGEIYPCKADIFEMTYEKVEYTATIENLTNYAENI
                     EKGHRCADEDNNKENKLELSAKLELDTKDFEENIKNATKEIEIFNKAVGTLEQKINGI
                     FGREKVNEVKINIPTISKSESVEEVNGKLLKELKSQKLIVSEKDKKFKKYDICFKNGE
                     CISGVIKEHVADKLTAYFCDSQSDIDCLRAIEDEEKLVMFNTANVQTITISDYIENEE
                     IDYI"
     gene            7357..7548
                     /locus_tag="ACGI59_002444"
     CDS             7357..7548
                     /locus_tag="ACGI59_002444"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9740228.1"
                     /translation="MSWLILQIVSIVVNVILIISLVSDIVLKIYLKSLKKEYEVAINK
                     ASNELLKKLNDELRKRKSL"
     gene            7666..8262
                     /locus_tag="ACGI59_002445"
     CDS             7666..8262
                     /locus_tag="ACGI59_002445"
                     /inference="COORDINATES: protein motif:HMM:NF018515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage scaffolding protein"
                     /protein_id="HFG9740229.1"
                     /translation="MEWLRKILESIKIEENKIDVEEILKSVNTEFPKYAVPKETFNKT
                     NEQLKEANKAIESFNSKMTQEDLEKIKTEHQAQIKKIEENHKIEVEKMQNESLKTRKL
                     SAVEKALLTNKAKHTDLLTNKFDLEKITIGEDGKIVGIEEQLKELQESYKDLFESSTT
                     ETTTQTNTPFYKYVPGGSGETNETANMETVVNGILGVK"
     gene            8286..9224
                     /locus_tag="ACGI59_002446"
     CDS             8286..9224
                     /locus_tag="ACGI59_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015981494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9740230.1"
                     /translation="MANILAYGQVLQQGLDKQATQELLTGWMDSNAKQIKYEGGKEVK
                     IGKLSTDGLGDYSRGSANAYVGGDVKFEYETKIMTQDRGRKFTLDAMDVDETNFLVTA
                     TTVMGEFQRLKVIPEIDAYRLSRLATIAISIEGDINVEYGYTVNSSTIINKIKTGIKI
                     IRENGYNGPLVCHLTYDSMFAIEEKVLEKLTAVTFAQGGIQTQVPSIDGCPLIKTPQN
                     RMYSSILLNDGTTSGQTAGGYLKGTKALDTNFIIAPVDVPLAITKQDKMRIFDPETNQ
                     TANAWSMDYRRYHDLWVTDNKANSVYANFKDAKPTA"
     gene            9237..9458
                     /locus_tag="ACGI59_002447"
     CDS             9237..9458
                     /locus_tag="ACGI59_002447"
                     /inference="COORDINATES: protein motif:HMM:NF019139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HFG9740231.1"
                     /translation="MFILIKENIERSVEDSFLKDKLIQDGFKLLEDKKNIDIENSTLE
                     ELKNLAKENGIEGYSKLKKDELIEKLNSI"
     gene            9487..9828
                     /locus_tag="ACGI59_002448"
     CDS             9487..9828
                     /locus_tag="ACGI59_002448"
                     /inference="COORDINATES: protein motif:HMM:NF016990.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HFG9740232.1"
                     /translation="MLDNIKLILNLKDDTHDSLIELYIKKYTTLVLAYCNIETLNSTL
                     EGIVEDKVIVKLKETVLVSSADNGISSISRGGYSVNYNVATAKTTDELMEIKLSQKDK
                     NILNNFRKVRW"
     gene            9830..10180
                     /locus_tag="ACGI59_002449"
     CDS             9830..10180
                     /locus_tag="ACGI59_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015981496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9740233.1"
                     /translation="MTEADILALTYFCKMTIRRCVSSKNEETGVTDFNESLVIAEDVP
                     CGLSGNIPNVIDTDITSSISVFELYCRPEVDLRVGDILDITLENGAVETFIVSKPFPY
                     SSHLQTNLTLKERY"
     gene            10181..10591
                     /locus_tag="ACGI59_002450"
     CDS             10181..10591
                     /locus_tag="ACGI59_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015981497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HFG9740234.1"
                     /translation="MIEFNSLDTLIRDLEREEREMSKNLRRAKNNIGNKLLRKVKPKT
                     PVAKVDGGTARKSWKYKELNLFEGVVSNNVEYIYHLEYGHRTRQGTGTSENYRPKPNG
                     ISFVPGVFMLARSVDEMSSIIDDELNQIIIDFWN"
     gene            10602..11054
                     /locus_tag="ACGI59_002451"
     CDS             10602..11054
                     /locus_tag="ACGI59_002451"
                     /inference="COORDINATES: protein motif:HMM:NF044614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6838 family protein"
                     /protein_id="HFG9740235.1"
                     /translation="MLSYKDILYSFTKELGNNFNEDIFVEGYNIQDNKKSCFFVQILP
                     EVAQTATKKTDIKSFLVDIKYLPDWKKKKTDLFDILNKLENIFTRNIKVKDRYLTFSK
                     KNGSIEKDEIGNYVQFLISINYHEQIYFEEEKHELMEELNMRFKGRSD"
     gene            11055..12125
                     /locus_tag="ACGI59_002452"
     CDS             11055..12125
                     /locus_tag="ACGI59_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HFG9740236.1"
                     /translation="MAGLVNINIEFKELATSFIQRSQTGIVAIILKDTTKMYKELTSE
                     DDIPISLSADNKKYIKYGFVGATDNEKILRPSKVIISTFTEDGKVEDILEELESVEFN
                     YLCMPEAIEAEKTKIVNWIKKIREEESTEAKAVLANIKADNEAVINFTENVTVGGEEI
                     TAEKYTPRIASLIASTPSTQSITYAPLNEVESITKIDKASADAKVQSGELILRRLSGA
                     IRIARGVNSLTTLTQEKGEIFQKIKLVDTKDLISKDIKDIYVKSYIGKVPNVYDNKCL
                     FIIAIQAYLAELAKQELIDSNFSVDIDIEKQKEYLEGKKVDTSKMKEDEIKEANTDSS
                     GFYLIKLKLVDAMEDINISVQM"
     gene            12141..12572
                     /locus_tag="ACGI59_002453"
     CDS             12141..12572
                     /locus_tag="ACGI59_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015981500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFG9740237.1"
                     /translation="MATSYEPRNVINGTYGEVWINNQQIAECKAMKAEIKFDKAEIVR
                     PRKIIKGQKIISASAEGSLTLYHVDSKILDYVTQIIKEGREPKFTITSKLSDPDSFGT
                     ERIAITGVSFDGLTIIDWENGKEGEKEVSFTFEDYNPIQTI"
     gene            12602..13069
                     /locus_tag="ACGI59_002454"
     CDS             12602..13069
                     /locus_tag="ACGI59_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015981501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9740238.1"
                     /translation="MSENKLEKEMVDKKEMTEVKNVVDLLLSLDADKVKMPSITHTIF
                     CKKLGIDVNFECKAIEPEFFDELQTSGLKIEKGSLKDLDNFKMKSNVILASCNLFKDD
                     KLLKHFQSPTPKELLRRMLLAGEINELYDKICELNGYRDDNKKDKEIEEKIKN"
     gene            13096..13242
                     /locus_tag="ACGI59_002455"
     CDS             13096..13242
                     /locus_tag="ACGI59_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9740239.1"
                     /translation="MYLMFRYHNMLPSDFFKLKYGEKHVIRAFMYQEIDERIEEIKSF
                     GKGL"
     gene            13246..16080
                     /locus_tag="ACGI59_002456"
     CDS             13246..16080
                     /locus_tag="ACGI59_002456"
                     /inference="COORDINATES: protein motif:HMM:TIGR01760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFG9740240.1"
                     /translation="MSAGSRALEAVIRMRDEASRTLRQVRDATRALQNQTNTTSQAQE
                     RLQEQFRKVSNAAKIAGVGIATGIGAGLVSASKAGAEFETAMTKTSTMFGDTKVDTEN
                     LNNKVLELSKNTGIAASSIGESLYNALSSGIPVTKDMGSAMDFMTKNAKLSKAGFTDI
                     DTALTATAKVLNAYKMDVSETDRVHKVMMQTQNKGITTVGELGATLAQVTPTASAMSF
                     SFEQVGASLANMTAQGTPTAQATTQLNSLLAELGKTGTVANKSLLDATKGTKYAGKSF
                     KELMQAGVPLNEILNLMDGSAKKNKKSLIDMFGSIEAGKAALALSGQNSEQYTNNLKA
                     MSTQADVVSSAYAKMSNTLESKVGILKESFKNLGIEIYSKLKEPLKNAAETGIQCLSD
                     LDKQFSSGSLKAGISQIAQSFGDLTSAIIKIATKALPTMVKSLSWVLKNGPTIASIFV
                     SIKTASIMTSAVKSIVALKKAWVAAKLAVRVYMVGMAEAGTVLSGFQVLVGVLTREMT
                     IAQGVTQVWSATLATLGGPVGVAIIAIVALVAGLVVLWNTNKGFRDFVINTWNNIKET
                     ATKVWGGICNFFTQTIPQAWNDLCTSFSNAGQWFGEMWNNIKQAFINGWNAIVAFFTQ
                     TIPMWINSIGVWFGQLPAKIGYGLGFALGKIISWGISVWTYLVTNVPIWINNIGTFFA
                     QLPNKIWTWLVSTVQKIGQWGMAMIAYAQIYARMIINNIVVFFQTLPSRVWTWLTNTV
                     QKVVSWGSQMASKGREGAKKLITTVVDTLKSLPKKVMDIGKNIVKGLWEGITGAGDWL
                     KGKVGDFANGVINGFKDGFGVHSPSWKLRDLVGKFLPPGIWNGIKMTLPKLETNITGM
                     VDSLVKRMYNPKEVEESNYTSKYKEAIAQRTEQNTINKTDSKTTNNKESNNITININW
                     GGVTVKEEADMTKLTKMLVNEIKLNLAGGV"
     gene            16155..16955
                     /locus_tag="ACGI59_002457"
     CDS             16155..16955
                     /locus_tag="ACGI59_002457"
                     /inference="COORDINATES: protein motif:HMM:NF025826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HFG9740241.1"
                     /translation="MALFSSKENCVICGEKTKNLKIKDGYVCKKCLQRCTGIEYVLNP
                     AFGSAFLNKKNKEVTKEDILGLLEKRGNNLDELDNFNTTKQVSVYLEIDEVQRKCLIP
                     DGSIGKKINPKIFSFDEIVEYELVEDEESIIKGGLGKAIAGGVLFGGVGAVVGGVTGK
                     KKTKTVVNSLKIKITLNNLNYPNVYINLITTQTKTDSFIYKNAYDSAQQILSMLSIIQ
                     NGNNENSIEKNTESNSSLEQIKTLKELLDMGAITEEEFNAKKKELLNL"
     gene            17010..17201
                     /locus_tag="ACGI59_002458"
     CDS             17010..17201
                     /locus_tag="ACGI59_002458"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9740242.1"
                     /translation="MNKGIKFIGSLKRLNFLIQAKENLIKDIHEYSNNEEEIARYENL
                     DKAFEKTIIDEAKFLISLE"
     gene            17225..17854
                     /locus_tag="ACGI59_002459"
     CDS             17225..17854
                     /locus_tag="ACGI59_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFG9740243.1"
                     /translation="MWIRQANDTFRFPVFPSSFEINSKAIVNTSNVLKTGEIAVFGGV
                     GLRTTEISSFFPKNEASYCDYTGFPSPYDCVNKIQRWMNEGFILRFTITETNINFECI
                     ITDFQYEEKDCTGDVYFTLSLKEYKRIQIPKVSTNTDLSSSKDVPLTKGFEVKNKQRT
                     HKVGKGDSLWSLAKKYYGNGDLWKKIYDANKKLIKNPDIIKDGWVLVIP"
     gene            17869..18144
                     /locus_tag="ACGI59_002460"
     CDS             17869..18144
                     /locus_tag="ACGI59_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015981507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9740244.1"
                     /translation="MDKLRIISESNSLTKEDLTEKDLYCIGKFIQIGAIKSFMHNEKD
                     LDFPCTDCKYSDKCFGDNRESDFWDTFLKLSKLTDLKLSPFKGFNIN"
     gene            18403..>18740
                     /locus_tag="ACGI59_002461"
     CDS             18403..>18740
                     /locus_tag="ACGI59_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HFG9740245.1"
                     /translation="MIINRSKDSSNNSISFTSKDMGFLLTQSEVSYNFKDKLVEDIAK
                     QVFNDNKLAIGNIPKTNVKYTKMFIGVTGYDTIMSAYTEASKTTKKKYMIESNLDKFN
                     IIEKGTVTLNV"
BASE COUNT         7823 a         1964 c         3539 g         5414 t
ORIGIN      
        1 atatttaaaa agttagtaag tggtgataga gtaaatgttg aaagaaaagg tcagaatcca
       61 tttgatttta acaactatag taagttttta ttttcagcta acaatatacc tagaataaag
      121 gataagactg gagcagtact tgatagatta attattattc cttttaatgc tagcttttca
      181 gtaaatgata aggattttga cccttatata aagtacaaat taagagagca agaagctata
      241 gagtatttaa taaatctagg attagaaggc ttaaaaagag tattgaagaa tagaaaattt
      301 acagtaccag ataaggttaa aaaagaaata cttgaatatg aagaagtcaa taatcctatt
      361 ttaggatttt ttaaagaagt tgataagata gaaaatgaat caacaaaaga aatatacatg
      421 aaatatcagg agtattgtat tttaaatgga ttacaaccta tatcaaacgt agaattctca
      481 aggcaagttg ttaaaaagtt tggatatgaa ataaaggaca aaaaaattaa tgggaaaaaa
      541 tatagagtat tcatcaacgg ttcaagatag ttcaagatgg ttcaagatat aggttcaaga
      601 tgtaatgcag taatatcaat ggggttcaag atggttcaag atgtttttga cctttatata
      661 tatttaagta aaaaatcatc acaaaaagtt gatgattttg ctccctttat ataaagaata
      721 tatatataat agtatgggac tgaacaaatg ttcttgacta tatgtattga aattacttaa
      781 gttcaagagg ttcaacatct attttacatc ttgaaccatc ttgaacttga tttaattagc
      841 aagttatata atgaagttta attaaaaaaa taagttatat ttcattaata acttaacaaa
      901 ataattaaaa agaaaattct gttaatttgc aaggatgtga aaatgaagtg acagactatg
      961 aaaaattaga agaattgtta aactcatata gtaatttgga tattgaaatt agagaaatag
     1021 agttaaaagt aagagggagc agtttaagag gaatagaatt aaatggaatg cctaaaggaa
     1081 ataatgtttc ttcaccaata gaaaatgagt taatatatgt tgaaagattg gagaatgaga
     1141 aaatatatct acaaattaaa aaagaatcag ttaataatat gttaaattta ttagatgatt
     1201 ttgaaagaaa tttaatagaa ctaagatatt ttaaaaaact acaatataaa caaataagtt
     1261 atgaattaaa tataagcgaa ttgtatgtag gaaaaaagag aaaaaaggca ctagataaaa
     1321 tacttccctt tgctaaaaaa tataatctaa tatgaaagtc ttgtttatgt ccgattaatg
     1381 tccgatttat gaccgattaa tgattgattt agtatagaaa catgtcacat caatatacta
     1441 tgagataagt tataatggta gtatgaaaca agtgtataat atatcctctt gaaaaaggct
     1501 aagtacccct aacttagtct tttttatatt taaaaaaagt gaggtggtga tgtggcaaaa
     1561 tatgaatact ggataacaga agaaggatta attaagattg aaggatgggc aagagatggg
     1621 cttacagatg aacagatagc atttaatatt ggaataaatg tcaaaacact atatgactgg
     1681 aaaaagaaat atagtaatat ttgtaatgcc ttaaaaaagg gaaaagaagt aattgacaga
     1741 caggttgaaa atgctttact aaaaagagca ttaggttatg agtatgatga gataacatat
     1801 gaagaaggtc aagaaactaa aagagtaact aaacaagtag taccagatac tacagcacag
     1861 atattctggt tgaaaaatag aaaaccagct gaatggaggg ataaacaaat agtagaatca
     1921 actaatgaaa ttacaataaa taatccgttt aaagaactat ctacagaaga attaaaaagg
     1981 ttggcaaaat tagatgacga tggataaaaa gttaatacag ttagaagcga agaaagaact
     2041 tgcaagacgt gagttctttt atttttgcaa tttattagca ccaaaatttt ataaagaaga
     2101 tagaaagtat ttagttgaaa cctgtaataa gcttcaagat ttttattatt cagatgatga
     2161 agttttaatt ataaatatgc cacctagaca tgggaaaagt agaagtgcaa gtttatttgt
     2221 agaatggatt ttaggtaaaa ataaaaatga aaaaataatg actggtagtt ataatgagac
     2281 tctttcaact atgttttcaa agaatgttag aaatgctatt caagaagaaa aagccgatat
     2341 agacactatt atctatagcg atatatttcc taacacaaaa atcaaacatg gtgatggagc
     2401 tatgaattta tggtcattag aaggtggtta taataattat ttggcgacct ctccgagtgg
     2461 tacagcaaca ggttttgggt gttctcttat gattgtagat gacttagtta aaaatgcaga
     2521 ggaagcttat aatgagaatg ttcttgaaaa acattgggat tggtttacta atactatgtt
     2581 atcaagacta gaggaaaaag gaaaaataat aattataatg actagatggt ctagtaagga
     2641 cttagctggt agagtattag aacattacaa agaagaaggt aagaaggtaa gacatattaa
     2701 tatgaaagca ttacaagaag atggaaatat gctttgtgaa gaagtattat ctttaaagag
     2761 ttataaatct aaagtgagag ctatgggcga ggatattgcg agtgctaact accaacaaga
     2821 gcctattgac cttaaaggat gtttatacac taagtttaag acatatgaga agcttcctgt
     2881 tgatggagaa ggcaatttac tatttacatc tattaaagct tatgtagata cagcagatga
     2941 gggagcagat tacttatgtt ctatagttta tggagtgtat aacaaagaag cttatatact
     3001 agatgtttta tacactaagg aaagtatgga aataacagag tataaaacag ctaagatgtt
     3061 ctataataat gaagttaaca aagctgatat agaaagcaat agtggtggta gagcttttgc
     3121 aagaaatgta caaagaatat tgaaagaaaa gtttaaaagc aataaaacga ttattaaatg
     3181 gtttcatcag tcgaaaaata aaaatgctag aattttatct aatagtagtt gggtaatgga
     3241 acatatatat tttccaatta attggagaga taagtggata gagttttata aggctatggt
     3301 gagttatcaa agagaaggta agaacaaaca tgatgatgca ccagacgctt tgacaggggt
     3361 tgcagaaaag gcactgaaag gtcaaggatt atcagtattt aagtaataat ttaagtaata
     3421 taggtggtgg tgatgtggag ttagaaaaaa taagagcaat aataagtgct gatatagcta
     3481 gaaggcaaga gatattacag gctaaatcat attattataa tgaaaatgat atattgaaaa
     3541 agggtgtagt tgtacaaaac agagatgaga atcctcttag gaacgctgac aatagaatca
     3601 gtcacaactt tcatgaaata cttgtagatg agaaagcttc ttatatgttc acctatccag
     3661 ttctttttga cattgacaat gacaaagaat tgaatgagaa ggtaacagat gttttaggga
     3721 atgagtttac tagaaaagct aagaatttag caatagaagc gagtaattgt ggtactgcat
     3781 ggcttcacta ctggatagat gaagaatata gtggagaaca ggtaactaat caaacattca
     3841 aatatggtgt agtgaataca gaagaaatta ttcctatata tcgaaatgga attgaaagag
     3901 agttagaagc tgttataaga tattatgttc agttagaaga tgtaaaaggt caaatacaaa
     3961 agcaggcata tacttatgtt gaattttgga cagataaaat attagataaa tataaatttt
     4021 ttggagtaac atgttgtggt tctcaaatag agcatataac agtacaacat agatttaatt
     4081 cagttccttt tattgaattt gctaacaaca taaaaaaaca aagtgactta tcaaaatata
     4141 aatcagtgtt ggatttatac gataagataa tgagtgggtt tgcaaatgat ttagaggata
     4201 tacagcaaat aatatacatc ttagaaaatt atggtggaga ggatacagca caatttttaa
     4261 atgaattgaa gagatataaa gcaataaaaa ctgaaacaga cagcgaaggc gatagtggtg
     4321 gtcttaaaac tatgcaaata gagatacctg ttgaagctcg aaaggtgata ttagagatac
     4381 ttaaaaaaca aatatatgaa agtggtcaag gattacaaca agatactgaa tcgtttggga
     4441 atgcaagtgg tgtagcactt aaattctttt atagaaagtt agaattaaag tctggactcc
     4501 ttgaaaccga gtttagaacc tcttttgata agctgattaa ggctatacta tattttttag
     4561 gagttacaga ctataaaaag atacaacaaa catatacacg caatatgatg tcaaatgatt
     4621 tagaggatgc agatatagca actaagtcag ttggcataat accaactaaa attattttaa
     4681 ggcatcatcc ttgggtcgac gaccccgaag aagctgcttt acttttaaga gaagaaaaga
     4741 aaatacaagc ttcaaaagta tctgatgatt ataataactt tactgaatag aggtgaagtt
     4801 atttgaataa taatattgag tactggaaag aaagagaaaa gcaaagatta aattcaagat
     4861 tgaaagaaga aaaagaggta ttaaaggaac tagataaaca atataaaatt gcaatgaaaa
     4921 atatagagaa agaaattgct aatttatttt ataaatatgc tgaacagaat aaattaacat
     4981 atgcagaaac acaaaaatat ttaactaata atgaatttaa cacatggcgc atggacatca
     5041 aacaatatat taaattaata gaacaaacaa gtgatgaaag attactgtta gagcttaata
     5101 cattagctat gaaaagcaga ataaacaggt tagaagaact attctaccaa atatctaaag
     5161 agataaataa tacattcgac attcaaaata atagagtaga aaagctatta gaagaatctg
     5221 tgaaagatag ttactataag agtatatatg aaactcaaaa acacgttggt gttggggtaa
     5281 gcttcactaa gcttgataga gaagctttaa aggatataat tacatatcct tggagtggta
     5341 aaaacttttc tcaaaggata tggaaaaaca gagatttatt aagtgaagtt atcaaggaag
     5401 aaattactca aatggttata agaggagaaa gtttgaaaaa gatttctaat agagtatcgg
     5461 aaaaaatgga ctctagttat gaaaatgcaa taagattagt acaaacggaa cattctcatt
     5521 ttatgtcaga agctgataaa aaagcatatg aaagtcaaaa agtagataaa tatcaattct
     5581 tagctacgtt acaggataat acttgtaaaa gatgtagaag tgtagacatg aaagtatatt
     5641 tagttaaaga agcgaaagaa ggagaaaatt atcctccgct ccatcctaga tgtcggtgta
     5701 caactattcc atatttcgag gatgaaaaag ggggaacaag gacagcaaga ttgctaaaag
     5761 gtaagacata tgaagttcct ttcaatttat catataatga atggtataaa aatcacgttg
     5821 aagttgctat aaatagagag aaggaaaata aaaataatgt tataataaat aatataaaga
     5881 aggatataaa agatggcaaa tataatctaa atattcataa tggtaaacaa ggaaagcatt
     5941 ttaaggacca taataattat acagaaggta ggagctactt aactgtatca aaagaagaag
     6001 ttcaagaact tattaacaaa tatgcaggca caggaatatt ggagcttgat agaaatggta
     6061 agtttaaaaa taaagaatta ataacatgtg aaaaaaatat aggtgtaaat ataagcaatc
     6121 taacaggaga agaaacggaa actaataagt tttatataca ctatagtaaa aatggtactc
     6181 atatagtgcc aacaatgaaa ggagttgaaa aggatgagta aaaatttaat taattatatg
     6241 agtgagaagg ttaaaattat agatatagac aataaagaat ggattggtta tgtaaaaact
     6301 tatacaccag ctatagatag tgataatgaa ataaatgaga ttggactaaa acaagaggat
     6361 tgtttaatat cttttcaaga agatgaaata aaaagtatag aaattatata gaaagcactt
     6421 tctaagttta aatagtaagt gcttttattg tgtaaaaaat taaggaggaa taaataatat
     6481 ggctaagttt aaaaagaaac cagttgaggt ggaagctttc agattgggct atgatataga
     6541 accagaatgg ttttttgaaa atagtagagt ttgtaatttt atacaagaaa aatgtattga
     6601 tggtaatgta agttgcgatt taaagacact ggaaggtact atgagggcta ataagggcga
     6661 ttacattata caaggagtaa aaggagaaat atatccttgc aaagcagata tatttgaaat
     6721 gacatatgaa aaggttgaat atactgcaac tattgaaaat ttaacaaact atgctgaaaa
     6781 tatagaaaaa ggacatagat gtgctgatga agataacaac aaggaaaata aattagaact
     6841 ttcagctaag ttggagctag atacaaaaga ttttgaggaa aatataaaaa atgcgacaaa
     6901 agaaattgag atatttaata aagcagtagg cacattagag caaaaaatta atggaatatt
     6961 tggtagagaa aaggtaaatg aagtaaaaat aaatatacca acaatatcaa aatctgaaag
     7021 tgtagaagaa gtaaatggaa agttacttaa agaattaaaa tcacaaaaac ttatagtttc
     7081 agaaaaagat aaaaagttca aaaaatatga tatatgtttt aaaaatggtg agtgcatatc
     7141 tggtgtaatt aaagaacatg tagctgataa actaaccgct tatttttgtg attctcaatc
     7201 agatatagat tgtttgagag ctattgaaga tgaagaaaaa ttggttatgt ttaacacggc
     7261 taatgtccaa actataacaa ttagtgatta tattgaaaat gaagaaattg attatatata
     7321 ataatttctt tattttgtaa aaatgaggtg agttaaatga gttggttaat actgcaaata
     7381 gtaagtattg ttgtaaatgt aatacttatt atcagtttag taagtgatat agttttaaaa
     7441 atatatctaa aaagtttaaa aaaagaatat gaagtagcta taaataaagc ttctaatgaa
     7501 cttttgaaaa aattaaatga tgaactaaga aaacgtaaaa gtctataaag gcttttttat
     7561 tttgctcttt ttaaaaaagt tgtagagcat aaagaacaaa gtaactcctt tagttggaga
     7621 acaactataa aaatctatgg agaaataaga agggatgatg attaaatgga atggttaaga
     7681 aaaatattgg aaagtattaa aatcgaagaa aataagattg atgttgagga aatattaaaa
     7741 agtgttaata ctgaatttcc taagtatgca gtgcctaaag aaacttttaa taaaacaaat
     7801 gaacaattga aagaagctaa caaagctatt gaaagcttta atagcaaaat gactcaagaa
     7861 gatttagaaa agataaaaac agaacatcaa gcacaaataa aaaagataga agaaaatcat
     7921 aagatagaag ttgagaaaat gcaaaatgaa agcttaaaga caagaaaatt aagtgctgtt
     7981 gagaaagctt tattaactaa caaagctaaa cacactgact tactaacaaa taagtttgac
     8041 ttagaaaaaa taactatagg tgaagatggc aagatagtag ggatagagga acaattaaaa
     8101 gaattgcaag aaagctacaa agatttgttt gaaagtagta caactgaaac tactactcaa
     8161 acaaatacac ctttttataa atatgtacca ggtgggagtg gagaaacaaa tgaaactgca
     8221 aatatggaaa ctgtagtgaa tggaatacta ggagttaaat aatataatta agagaggatg
     8281 attagatggc taatatacta gcatacggac aagttttaca acaaggattg gataaacaag
     8341 caacacaaga attattaact ggttggatgg attctaatgc taaacaaata aaatatgaag
     8401 gaggaaaaga agtaaaaata ggtaagcttt ctacagatgg tttaggagat tattcaagag
     8461 gttcagctaa tgcttatgtc ggtggagatg ttaaatttga atatgaaact aaaataatga
     8521 ctcaagatag agggagaaaa tttaccttag atgctatgga tgtagatgaa acaaatttct
     8581 tagtaacagc aacgactgtc atgggagaat ttcaaaggtt aaaagtcata ccagaaatag
     8641 atgcttatag attgagccgt ttagcgacta ttgctataag tatagaagga gacattaatg
     8701 ttgagtatgg ttacacagta aattcaagca caataataaa taaaataaaa acaggtataa
     8761 aaataattag agaaaatgga tacaatggac ctctagtttg tcatttaact tatgacagta
     8821 tgtttgcaat agaagaaaaa gtcttagaaa aattaacagc agttactttt gcacaaggtg
     8881 gtatacaaac acaagtgcca tcaattgatg gttgccctct tataaaaaca cctcaaaata
     8941 gaatgtattc atctatttta cttaatgatg gtactacttc tggtcaaaca gcaggaggtt
     9001 atttaaaagg cacaaaagca ctagatacca actttataat agcaccagtg gatgtacctc
     9061 ttgcaataac aaaacaagat aaaatgagaa tatttgaccc agaaacgaat caaacagcaa
     9121 atgcttggtc tatggactat agaagatatc atgatttatg ggttactgat aataaagcta
     9181 attctgtata tgctaatttt aaagatgcta aacctacagc ttaggaagtg atttaaatgt
     9241 ttatattaat taaagaaaat atagagcgta gcgtagaaga ttctttttta aaagataagc
     9301 taatacaaga tggatttaag ttgctagaag ataagaaaaa tattgatata gaaaattcaa
     9361 ctcttgaaga actgaaaaac ctagcaaaag aaaatggtat agaaggttat tcaaagctga
     9421 aaaaagatga attaatagag aaattaaata gtatttagtt tctctatttt actttgaggt
     9481 gattaaatgc tagataatat aaagttgatt ctaaatctaa aagatgatac tcatgatagt
     9541 ttaatagagt tgtacattaa aaaatatact actctagttc ttgcatactg taatatagaa
     9601 acgcttaatt ctactcttga aggtattgta gaggataaag ttattgttaa gttaaaagag
     9661 actgtattag tcagtagtgc tgataatggt attagctcaa tttctcgtgg tggttattct
     9721 gtaaattata atgttgcaac agctaaaaca acagacgagt tgatggaaat aaaactatct
     9781 caaaaggata aaaatatttt gaataatttt agaaaagtta ggtggtaaaa tgacagaagc
     9841 agatatacta gcactgacat acttttgtaa aatgacaata agaaggtgtg taagcagtaa
     9901 aaatgaggaa acaggagtta cagattttaa cgagagtctt gtaattgcag aagatgtgcc
     9961 ttgtggtttg agtggaaata tacctaatgt catagataca gatataacaa gctctatctc
    10021 agtatttgag ttatattgta gacccgaagt ggatttgaga gttggagata tactcgatat
    10081 aactttagaa aatggggctg tagaaacctt tattgtatct aaaccatttc cttattcaag
    10141 tcatttacaa actaatctaa ctttaaagga aagatattaa atgatagagt ttaatagtct
    10201 agacacatta ataagagatt tagaaagaga agaaagggaa atgtcaaaga atttaagaag
    10261 ggctaagaac aatataggta ataaactact tagaaaagta aaacctaaaa caccagttgc
    10321 taaagttgat ggaggaacag ctagaaagag ctggaagtat aaagagctta atctatttga
    10381 gggtgtagta tcaaataatg ttgaatacat ttatcattta gaatatggtc atagaactag
    10441 gcaaggaaca ggaactagcg aaaactatag acctaagcct aacggaatta gttttgtacc
    10501 aggtgtattt atgttggcaa gaagcgttga tgaaatgagc agtataattg atgatgaatt
    10561 aaatcaaata ataatagatt tttggaatta gaggtgatgc gttgttaagt tataaagata
    10621 tactatactc gtttactaaa gaattaggta ataattttaa tgaagatata tttgtagaag
    10681 gatataacat acaagacaat aaaaagtctt gtttttttgt gcagatattg ccagaagtgg
    10741 cacagacagc gactaaaaag actgacataa aaagcttttt agttgatata aaatatttgc
    10801 ctgactggaa aaagaaaaaa acagatttat ttgatattct aaataaatta gagaacatat
    10861 tcactagaaa tataaaagta aaagatagat atttaacttt cagtaagaaa aatggaagta
    10921 tagaaaaaga tgaaatagga aattatgttc aatttcttat atctataaat tatcatgaac
    10981 aaatttattt tgaagaagaa aaacacgaat taatggaaga attaaatatg agatttaaag
    11041 gaaggagtga ttaaatggct ggattagtta atataaatat agaatttaaa gaactagcta
    11101 caagctttat acagcgttct cagactggaa tagtagcaat tatattgaaa gatacaacaa
    11161 agatgtataa agagcttaca agtgaagatg atataccaat ttcattgagt gctgataata
    11221 aaaaatatat taaatatggc tttgtagggg ctaccgacaa cgagaagata ttaagaccaa
    11281 gcaaagttat tataagtact ttcacagagg atggaaaggt tgaggatata ctagaagaat
    11341 tagaatctgt agaatttaat tatctttgta tgccagaggc tatagaagca gaaaaaacaa
    11401 aaattgtgaa ttggattaag aagataagag aagaagaaag tacagaagct aaggcagtat
    11461 tagcgaatat taaagctgat aatgaagcag ttattaactt cactgaaaat gtaacagttg
    11521 gtggagagga aataacagca gaaaaataca caccacgtat tgcttctctt atagcatcta
    11581 ctccaagcac acaatcaatt acttatgctc ctcttaatga agttgaatct attacaaaga
    11641 tagataaagc tagtgcagac gctaaagtac aatcgggaga attaatacta agaagattgt
    11701 caggggctat aaggattgct agaggtgtaa attctcttac aactctaaca caagaaaaag
    11761 gagagatatt tcagaagatt aagcttgttg atactaaaga cttaataagc aaagatataa
    11821 aagatattta tgtaaaaagt tatataggaa aagttccgaa tgtttatgat aataagtgtc
    11881 tatttataat tgcaatacaa gcgtatttag ctgaattagc taaacaagaa ctaatagatt
    11941 caaatttttc tgttgatatt gatatagaaa aacaaaaaga atatttagaa ggtaaaaaag
    12001 tggacacaag taagatgaaa gaagatgaaa taaaagaagc taatacggat tcaagtggat
    12061 tttatttaat aaaattaaaa ctagttgacg ctatggaaga tataaatata agtgttcaaa
    12121 tgtagaaagc aggtgaaaag atggcaacaa gttatgagcc tagaaatgtt ataaatggaa
    12181 catatggaga ggtttggatt aataaccaac aaatagctga atgtaaagct atgaaagctg
    12241 aaataaaatt tgataaagct gaaatagtta gacctagaaa aataataaaa ggtcaaaaaa
    12301 taattagtgc tagtgcagaa ggttctctaa ctctatatca tgtagattca aaaatattag
    12361 attatgttac acagattatc aaagaaggta gagaaccaaa atttacaata acaagtaaat
    12421 tgtcagaccc agattctttt ggaacagaga ggattgcaat aactggtgtt agttttgatg
    12481 gacttactat tatagactgg gaaaacggaa aagaaggaga aaaagaagtc tcatttactt
    12541 ttgaagatta caatccaata caaacaatat aaaaataatt aaggataaaa ggagaattaa
    12601 tatgagtgaa aataaattag aaaaagaaat ggtagataaa aaagaaatga cagaggtaaa
    12661 aaatgttgta gatttgttat taagtttaga tgctgataag gtaaaaatgc catctataac
    12721 acatacaata ttttgtaaga aactaggaat agatgtgaac tttgaatgta aagcaataga
    12781 gccagaattt tttgacgagc ttcaaactag tggtttaaaa atagaaaaag gttctttaaa
    12841 ggatttagac aattttaaaa tgaaatctaa tgtaatacta gcatcatgta atttatttaa
    12901 agatgataaa ttattaaaac attttcaatc tccaacacca aaagaacttt taagaagaat
    12961 gttacttgca ggggaaataa acgaattata tgataaaatt tgtgaattaa atgggtatag
    13021 agatgataac aaaaaagata aggaaataga agaaaaaata aaaaactaat caaatcggat
    13081 gaagaaatca atttaatgta tctaatgttt agataccata acatgttgcc atccgatttt
    13141 tttaagctta aatatggtga aaagcatgta attagagctt ttatgtatca agaaatagat
    13201 gaaagaatag aagaaataaa gagttttgga aaggggttgt agagtatgtc agcaggtagt
    13261 agagctttag aagctgttat aagaatgcga gatgaagcta gtaggacgct aagacaagtt
    13321 agagatgcta ctagagccct tcaaaatcaa actaatacaa catcacaagc acaagaaaga
    13381 ttacaagaac aatttagaaa agtaagcaat gcagctaaaa tagcaggagt gggaattgca
    13441 actggaatag gtgcaggact tgtttctgca tctaaagcag gtgcagaatt tgaaactgca
    13501 atgactaaga cttcgacaat gtttggagac actaaagtag atacagaaaa cttgaataat
    13561 aaagtattgg agttatctaa aaatacagga attgcagcat cttctattgg agaaagtcta
    13621 tataatgctt tatcttcggg cattcctgtc acaaaagata tggggtcagc gatggacttt
    13681 atgactaaaa atgctaaatt atctaaagca ggatttacag atatagacac agctttaaca
    13741 gcaactgcaa aagtgttgaa tgcctataaa atggatgtat cagaaacaga tagagtacac
    13801 aaagttatga tgcagacaca aaataaaggt atcactacag tcggagaatt aggagcaaca
    13861 ttagctcaag tgactccaac tgcatcagca atgagcttta gttttgaaca ggtaggagca
    13921 tcacttgcta atatgacagc acaaggaaca cctacagcac aagcaacaac acaattaaat
    13981 agtttacttg ctgaacttgg gaaaactggt acagtagcaa ataagtcttt attagatgcc
    14041 acaaaaggga ctaaatacgc aggaaaatct tttaaagaat taatgcaagc aggagtgcca
    14101 cttaatgaaa ttttaaatct catggatgga agtgctaaaa agaataaaaa aagcttaata
    14161 gatatgtttg gtagtattga agcagggaaa gctgcactcg cactttctgg tcaaaattca
    14221 gaacaatata ctaataattt aaaagctatg tctacacaag ctgatgttgt ttcaagtgca
    14281 tatgcaaaga tgtctaatac attagaatct aaagtgggga tactaaaaga aagttttaaa
    14341 aatctgggta tagagatata tagtaaacta aaagaacctc ttaaaaatgc ggctgaaaca
    14401 gggatacaat gtttaagtga cttagataaa cagttttcaa gtgggtcact taaagcaggt
    14461 atttcgcaaa ttgcacaatc ttttggagat ttaacatctg caattataaa aattgcaaca
    14521 aaggcattac caactatggt taagagtctt agttgggttc tgaaaaatgg acctactatc
    14581 gctagtattt ttgttagtat aaaaacagct tccataatga ctagtgcagt taaaagtatt
    14641 gtagcattaa aaaaagcttg ggttgcggct aaattagcag tgcgggttta catggttggc
    14701 atggcagaag caggcacagt attaagtgga tttcaagttt tagttggagt tttaactaga
    14761 gagatgacta tagctcaagg tgtaacacaa gtttggagtg ctactttagc tacactagga
    14821 ggaccagttg gtgttgctat aattgctata gtcgcattag ttgcaggtct tgtagtcttg
    14881 tggaatacaa acaagggttt tagagatttt gttataaata cttggaataa tataaaagaa
    14941 acagccacaa aagtttgggg tggtatatgt aatttcttta cacaaactat tccacaagct
    15001 tggaatgatt tatgtaccag tttttcaaat gcaggacaat ggtttggaga aatgtggaat
    15061 aatataaaac aagcatttat aaatggctgg aatgctattg tagctttctt cacgcaaaca
    15121 attccaatgt ggattaatag cattggagtg tggtttggac aattacctgc aaaaattggt
    15181 tatggattag gatttgcatt agggaaaata atatcttggg gtatttctgt ttggacttac
    15241 ctagttacaa atgtgcctat atggattaat aatataggca cattttttgc acaattacct
    15301 aataaaattt ggacttggct agtaagcaca gtacaaaaaa taggtcaatg gggtatggct
    15361 atgatagcat atgctcaaat ctacgctaga atgattatta acaacatagt agtatttttc
    15421 caaactctac ctagtcgcgt atggacttgg cttacaaata ctgttcaaaa agtagtaagt
    15481 tggggaagtc aaatggcttc taaagggaga gaaggagcta aaaaattaat tactacagta
    15541 gtagatacat taaaatcttt acctaaaaaa gtgatggata taggtaaaaa tattgttaaa
    15601 ggtttgtggg aaggtataac tggagctgga gattggctaa aaggcaaggt cggagatttt
    15661 gcaaatggtg taataaacgg atttaaagat ggttttggag tacattctcc atcttggaaa
    15721 ttacgagatt tggtaggtaa atttcttcca cctggtattt ggaatggaat aaaaatgaca
    15781 ttgccaaaat tggagactaa tattactggt atggttgata gcttagttaa aagaatgtat
    15841 aatccaaaag aagtcgaaga aagtaactat acaagtaaat ataaagaagc tatagctcaa
    15901 agaaccgaac aaaatactat taataaaact gacagcaaaa ctactaataa taaagaaagt
    15961 aataatatta ccataaatat aaattggggt ggtgttacag ttaaagaaga agctgatatg
    16021 actaaattaa ctaagatgtt agttaatgaa ataaagttaa atttagcagg tggtgtatag
    16081 aaacatagaa ctctaaaata tgtgagtatg tgctataatc gtagtatatc attatgtttt
    16141 agggggataa atatatggca ttatttagtt ctaaagaaaa ttgtgtaata tgtggagaaa
    16201 aaactaaaaa tctgaaaatt aaagatggat atgtatgtaa aaaatgtttg caaagatgta
    16261 caggaataga gtatgtcctt aatcctgctt ttggttcagc atttttaaat aaaaaaaata
    16321 aagaagttac aaaggaggac attctaggtc ttcttgaaaa aagaggtaat aatctagatg
    16381 agttagataa cttcaacact acaaaacaag tatctgttta tttagaaatt gatgaagtac
    16441 aaagaaaatg tctaatacca gatggttcta tagggaaaaa aataaaccct aaaatttttt
    16501 cttttgatga gattgtagaa tatgaactag tagaagatga agaaagtata ataaaaggtg
    16561 gtttaggcaa agcaatagca gggggagtgt tatttggagg agtaggagct gtagttggag
    16621 gtgtaacagg gaaaaagaag acaaagacag ttgtaaatag tctcaaaata aaaataacac
    16681 ttaataactt aaattatcca aatgtatata tcaatttgat tacaacacaa acaaaaacag
    16741 attcttttat atataaaaat gcatatgatt ctgcacaaca aatattatct atgttatcta
    16801 taattcaaaa tggaaataat gaaaatagta tagaaaaaaa tacagaaagt aatagttcat
    16861 tagagcaaat aaaaacatta aaggaattgt tggacatggg agcaataaca gaagaagaat
    16921 ttaatgctaa gaaaaaagaa ttattaaatt tataatatat aaagcactta ctttaacaag
    16981 taggtgcttt tattatggag gtgattgtgt tgaataaagg cataaagttt attggctcat
    17041 taaagcgatt aaatttctta atacaagcaa aagaaaattt aattaaagat atacatgagt
    17101 attcaaacaa tgaagaagaa attgctaggt atgaaaattt ggataaagct tttgaaaaaa
    17161 ctataattga tgaagctaag tttttaatat ctttagaata gatatagagg tgatgtagtt
    17221 ggaaatgtgg attagacaag caaatgacac ttttagattt cctgttttcc cttcaagttt
    17281 tgagataaat agcaaagcaa tagtaaatac ctctaatgtc ttgaaaacag gagaaattgc
    17341 agtatttgga ggtgtaggtt taagaactac agaaatatct agtttctttc ctaaaaatga
    17401 agcaagttac tgtgattata caggcttccc atcgccatat gactgtgtaa ataaaattca
    17461 aagatggatg aatgaaggtt ttatattaag atttacaatt acagaaacga atataaattt
    17521 tgaatgtatt ataacagatt ttcaatatga agaaaaagat tgcacagggg atgtgtattt
    17581 tacattaagt ttaaaagaat ataaaagaat acagatacct aaagttagta caaatacaga
    17641 tttatcatca tcaaaagatg taccacttac aaaaggattt gaagttaaaa ataaacagag
    17701 aactcataag gtaggtaagg gtgacagtct ttggagtttg gcaaaaaaat attatggcaa
    17761 tggggattta tggaagaaaa tttatgatgc taataaaaaa ttaattaaaa atccagatat
    17821 tataaaagat ggttgggttc tagtaattcc ttaagcgagg tgatagagtt ggataaatta
    17881 agaataatat cagaaagcaa tagtttaacg aaagaggatt taacagaaaa agacttgtat
    17941 tgtataggca agtttataca aattggagct ataaaaagct tcatgcacaa tgaaaaagac
    18001 ttagatttcc cttgtacaga ttgtaaatac tcagataaat gttttggaga taatagagaa
    18061 tcagattttt gggatacttt tttaaagtta agtaaattga ctgatttaaa attatctcca
    18121 tttaaaggtt ttaacataaa ttaatcactt ataaatcgaa aagacaagaa ggtgatttag
    18181 attaataata taaaattaca ggtccatata aaaaatggag ctatatacaa cataacagat
    18241 atagtagaaa aggtaacttg gtcaggtgat tataagtcac catcaaggac actagagttt
    18301 tctatagtac agtcagcttc tgatattaat tttaggcaga tagacatacc aatagctagt
    18361 acagtatgtt tttatgtaga tgataaagaa atctatagag gaatgataat taataggtcc
    18421 aaagactcta gcaacaatag tattagcttc acatcaaaag atatgggttt tttacttact
    18481 caaagtgaag tatcatataa ctttaaagat aagttagttg aggacattgc aaaacaagta
    18541 tttaatgaca ataaacttgc aataggaaat atacctaaaa ctaatgttaa atacactaag
    18601 atgtttattg gtgtaactgg ttatgatacc ataatgagtg catatacaga ggctagtaag
    18661 acaacgaaga aaaagtatat gatagagtct aatttagata aatttaatat cattgaaaaa
    18721 ggtacggtta cactaaatgt
//
LOCUS       DAYAPA010000077        18554 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.077, whole genome shotgun sequence.
ACCESSION   DAYAPA010000077 DAYAPA010000000
VERSION     DAYAPA010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18554
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.077"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            359..1381
                     /gene="ccpA"
                     /locus_tag="ACGI59_002462"
     CDS             359..1381
                     /gene="ccpA"
                     /locus_tag="ACGI59_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator CcpA"
                     /protein_id="HFG9740246.1"
                     /translation="MKMKGNITIKDVAKQAGVSISTVSRVINDSKPVTDEVKQKVLEV
                     IKETGYIPNPLARSLVTKKSQLIGVIVPEVSDSFVNEVLNGIEEVAKMYDYDILLANT
                     YSDKEQELKSINLLRAKQVEGIVMISWIVEQEHINYIQNCGIPATYISKTARNYDIYT
                     VSTSNEEATFDMTEHLIKKGHEKIAFIMTSKDDTVLEMERLAGYEKALSNNNIELDKS
                     LIKYGGTDYESGYNSMKELLDDGIIPHAAFVTGDEAAIGAINAICDAGYKVPEDISVA
                     GFNDVKIARMYRPKLTTVYQPLYDMGAVAIRMVIKLINKELIENKKIELPYRIVDRES
                     VTERKK"
     gene            complement(1504..1902)
                     /locus_tag="ACGI59_002463"
     CDS             complement(1504..1902)
                     /locus_tag="ACGI59_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9740247.1"
                     /translation="MKKLDTESLEKMAKQKNIDKDKIEKMADSYKGKSENELMEELIK
                     IGKNLDGRDEVVSKFKAFLDENQRKKLDNIMEKISDAENQRDTKPAKTKKAKPTKGKS
                     NSPAPKNTPGSQQKKSKSLFKKTKKSNPHE"
     gene            2114..3025
                     /locus_tag="ACGI59_002464"
     CDS             2114..3025
                     /locus_tag="ACGI59_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9740248.1"
                     /translation="MNNLLFLIGVIALSNGNISIFDDKNKSKGNNRVRKNKKNANDKL
                     SQSTHGNLRFDANDIKRGLRLMDLSEEDLEMGMEIITRTKKYMSRDERKILIKIESVL
                     DLVRGIKKLNNIDMVDTEDETDFFRKMDSDDKKNMMIKEIIDVFPEKRKNSVEKAIGM
                     KKKIDLFAELFLPDDFGEGGFSLSSLANINNLGSMNNLKLLGSLLRGDDSNNDDEDNN
                     DELNYEDEELDCEDKELDDDYDELTDEGEMEDNEDELVEDKNTGYENIENDEDIVYDE
                     ELYNINEQESEYNYNEKNTKTRAKKKR"
     gene            3140..3320
                     /gene="ssrS"
                     /locus_tag="ACGI59_002465"
     ncRNA           3140..3320
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACGI59_002465"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            3503..4720
                     /gene="cdeC"
                     /locus_tag="ACGI59_002466"
     CDS             3503..4720
                     /gene="cdeC"
                     /locus_tag="ACGI59_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium morphogenetic protein CdeC"
                     /protein_id="HFG9740249.1"
                     /translation="MQDYKKNKRRMMNQPMSTMNEEEVYTDEINSEDMRGFKKSHHHN
                     GCNTDNKCECHDDCNPCNPCNPCKPNPCNPCKPNPCDDNCGCHDNCKCDCEPCEMDSD
                     ECFENKCGPECCNPISPRNFSVSNAVPFAIEANRIFDTMQFQTFTDATGPNGEPLTFE
                     TEVVEVFGSVPSAGQASVTIEKICLSNDGIVIDTGMTTLEDFDLDPLGDIVGRNCETT
                     FEFAVCGERNAECCRQGKGKSVAYKQRGLTVAVRNLVLELRGRCGCTEFVALAFPAVR
                     AGGGCKRRVDYVEFTFNTLSAPICLPADGRAVTLRQEYQTNLTVDCIGKSILKLECNE
                     CCEPFYELIIPNDIDLVLCLQETVSTLISEQIVVLASPNPIQPRLVDTFSKVCDFSQC
                     GPNHGSGKPSCHR"
     gene            complement(4821..6143)
                     /locus_tag="ACGI59_002467"
     CDS             complement(4821..6143)
                     /locus_tag="ACGI59_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HFG9740250.1"
                     /translation="MKVPYLVLSTVILFISNFIVRIFGFLYKIFLSRALGETGLGIYH
                     MIFNFLMICLAVTTTGIPTALSCLVAKRKALNDRHNTNALFISTLYISFFVALIISIV
                     ASFNSSFLSLKFLKDAKLNLFILAVCPAIVIITLSNVLRGYYYGIKNVKIPAIGQIIE
                     QIGKILFVFLLVMYINNKSMNCYIALLGISIGELSNIIFMLICLWRDSSFDNRYIISI
                     KDFYNSSMETLKMSIPITCNRMSNILLQSISSMMIPSRLALSGMTYQQSLSMYGIVSG
                     MVMPFIFLPFTVGSALIVNLIPTISQEMALRKRKSVIKKIKYSILLTLFVGILSSIFF
                     YFFGKDLCILVFKNTLAGEYLKAMFLVPLFMSLNQTLSGILHSIRKELASSINTITGM
                     LIQLIALYVFLPIPGLNIYAYIYTMTIVSIFTCLLHTIVLFKSLKSIR"
     gene            complement(6194..6988)
                     /locus_tag="ACGI59_002468"
     CDS             complement(6194..6988)
                     /locus_tag="ACGI59_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HFG9740251.1"
                     /translation="MFYDYHMHSSFSTDGKSTMEEMVKKSIELGLEEICFTDHVDYDV
                     YADDSFSIVYEDYFKSLETLQSKYKDKISIKKGIEFGVQTQLMDTYKKEAHQYPLDFI
                     ICSIHAIDTMDLYLGNYFKDKTQHEVYENYYLYLYNIVKNYKDYSVLGHLDLIKRYAP
                     YDTILDDRLFSDIIEETLKQAIYDGKGIEINTSCYRYNLPDLTPSKYILQMYKDLGGE
                     IITTGSDSHHISQVACEFDYIYSLLKNMGFKYVSKFNKLKPEFIKL"
     gene            7098..8543
                     /locus_tag="ACGI59_002469"
     CDS             7098..8543
                     /locus_tag="ACGI59_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFG9740252.1"
                     /translation="MEKYRIDLNEKKSTKYIQIFNHIKELIINKELVEHEKLPPIRKL
                     SNFLQVNNTTIVKVYELLENEGYVYKIIGSGTFVSSLNSYKSGIDNRKNKIIVQREDL
                     IHFDNGNPSNDMFPIDSFKNAVNMALSKDGSSIFEYDEGLGSEELREKMVEYLSDGNI
                     KTTKENIQIISGAQQGIDIVCKGLISYSDVVFMEEPSYNGAIEVFKSRGAKIISIPML
                     DDGIDIGILKLKLEKIKPRLIYIMPNFQNPTGISYSTYKKKKLIELAEEHDFYIIEDD
                     FISDFVFESKDNRTIRSYDDKNRVVYIKSFSKILMPGLRIGIVEMPNELLKKVLWAKY
                     SSDISTPGLIQKSMYYYMNNFDWKNYLSHIEKIYTDKYKLAKNLINDKLSGKLKVRKS
                     CGGINFFLELPRGYTSQAFTNFMLNKGVSMLPGTYFFDNLVDDRFFRINIARPSMEEL
                     EKGISIISDNIDEFFCEYKNKLDIKSNKLFY"
     gene            8572..8781
                     /locus_tag="ACGI59_002470"
     CDS             8572..8781
                     /locus_tag="ACGI59_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 domain-containing protein"
                     /protein_id="HFG9740253.1"
                     /translation="MFDKKKLDRINELAKKNKEGILSADEIKEREILRKEYLENFRAH
                     FRSRLDSVKVVSPEEYEQYMKNNKN"
     gene            8801..10195
                     /locus_tag="ACGI59_002471"
     CDS             8801..10195
                     /locus_tag="ACGI59_002471"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFG9740254.1"
                     /translation="MNLKYEFKNAWDFERKENTIEQIMQYSSNYMEFLSKSKTERLSV
                     KEIIKLAKENNYISIDEAMEKGSISCGDKIYVINKEKAVALFVIGKNYIEKGMKIIGS
                     HIDSPRLDLKPNPLYQESNLGFFKTHYYGGIKKYQWTAIPLALHGIVILNDGTKVDIS
                     IGEEDSDPVFCVTDLLIHLAGDQMQKKLSEGISGEALNILIGNMPLEDEEKEPITANI
                     LKILNEKYNIVEEDLLSAEIEVVPAGKARDLGLDRSMVLGYGHDDRVCSYAAVKAILE
                     TEQPEFTSVALCVDKEEIGSKGNTGMHSKFFENTVAELIALEGDYCDIKVRRALANSK
                     VLSADVSAGYDPNFGEAYEKRNSAYMGNGVVLTKYTGSRGKSGCNDANAEFMSEVKRI
                     FNKGNVIWQTAELGKVDQGGGGTIAHILANQGAEVIDCGVGVLNMHAPHEIVSKVDIY
                     EMYKGYKAFFNINL"
     gene            10435..11187
                     /locus_tag="ACGI59_002472"
     CDS             10435..11187
                     /locus_tag="ACGI59_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFG9740255.1"
                     /translation="MDEKRQKKEMDLEVNSFVPLYQQLYDNIKKQIASGIYKPGDKLP
                     SEGDLCKEFNISRITVRNALNELVKEDILCKKRGKGTYVTIPERIEATCAGNSFTNSC
                     HRINAKPSTKIISVLIKKADKQVAEALSIELEEKVICIKRLRLIDEVPVIFEVDYFRI
                     DYMFLLKEELEDKSLMEVISNNISTLPKRVENIFEVKHSNKEYSDHLKCASNMPLLKV
                     RQSVYTENNDVLYYNEQFIRSDKYKYAVSAEI"
     gene            11213..11626
                     /locus_tag="ACGI59_002473"
     CDS             11213..11626
                     /locus_tag="ACGI59_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFG9740256.1"
                     /translation="MKKIILASHGDFSKGLLNAVKMIVGDLADCVSSYGLYPGQSASD
                     FALELEKTISEDKECEYIILSDLYGASVCTAMLRLTNLHNVRLFSGMNLNMVLELLTR
                     FSDELTVEDIDQLVEESRRGIQSVTLQIKEEEEVF"
     gene            11630..12373
                     /locus_tag="ACGI59_002474"
     CDS             11630..12373
                     /locus_tag="ACGI59_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtpA/SgcQ family protein"
                     /protein_id="HFG9740257.1"
                     /translation="MKKKGILSVFKSKKPIIAMIHLKGDTPEDIFERAKKEITIFEEN
                     GVDGIMLENYYGNYYDLERILEYVSKANLSIPYGVNCLNVDTMGFELATKYNASYIQV
                     DSVVGHVKPRDEATLEEFFKLQRSKCPAYLIGGVRFKYQPVLSENDVEEDLKIGMTRC
                     DAIAVTENATGQETSMEKIELFRKNLGDFPLVIAAGVTLENAKKQLKLGDMAIIGSYF
                     KDNYKDFGDVSVEHVKTFMDEIKKIREEL"
     gene            12373..12843
                     /locus_tag="ACGI59_002475"
     CDS             12373..12843
                     /locus_tag="ACGI59_002475"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFG9740258.1"
                     /translation="MIKLVRVDHRLIHGQVAFTWTKFLSTDCILIASDDLLKDELRMA
                     GLKMAKPSNVKLVMKSIADSIKALNSGVTDKYNLLILCESVEDVYRLAKEVKAIKSIN
                     LGGTKSDDNRENISKAVHVSKDDIKMIKELDSEGVNVFVQLVPDDDATNVMKLI"
     gene            12860..13618
                     /locus_tag="ACGI59_002476"
     CDS             12860..13618
                     /locus_tag="ACGI59_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFG9740259.1"
                     /translation="MEFTQVILITLIAFFAYMHSFVGSTMHNRPIVVAPLVGLALGNL
                     HTGIVIGSTLELVFMGAFPVGASNPPDFVSGSIIATAFVILTGQDVSAAVVLAVPIAT
                     LVLLIDNFLMTVVLTWGAHIADRYAEEGNIEGVERVQLLFGIGNKLILAIIVGIGFSL
                     GVPVIEKILSFIPSYVTHGMDVAAGVIPAIGFAMLARMMLNKKTVAFLLLGFILVAYL
                     NITVTGVALFGLAIALIYVNFVGEKEVIVDDNEF"
     gene            13602..14444
                     /locus_tag="ACGI59_002477"
     CDS             13602..14444
                     /locus_tag="ACGI59_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFG9740260.1"
                     /translation="MTTSSKKLETISPDSKITRKDFWKCFRRSLTLDSSWNYERMQNI
                     AYAYMMAPIIRRLYKDDKEKKSKALKRHLEFMSVTPHISTLLVGISGAMEEENAKNKE
                     FDANSINAVKSSLMGPVSGIGDSFFWGTLKLIAAGVGIALASQGNIMGPILFLLIINV
                     PHFIIRYICLDKGFKYGTQFFKDVSGSSIVSKVMEAASMLGLMVIGGMTASNVMLKLS
                     VNVGSGEWAEPIQTYLDQIMPCMLPAMIFGIMYWLLGKKVKTTTILISVMIICIVLAA
                     IGVV"
     gene            complement(14604..15509)
                     /locus_tag="ACGI59_002478"
     CDS             complement(14604..15509)
                     /locus_tag="ACGI59_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism-associated LysR family
                     transcriptional regulator"
                     /protein_id="HFG9740261.1"
                     /translation="MDFKQLEVFVAVAKHQSFSKAARELFLTQPTVSAHIQNLERELE
                     TVLINRSNKVITLTKSGEILYEHAIYILNNCKRAIYDIKEYSGKIEGIIDIACSSIPE
                     TYILPDFMKSFSMSYPDVKFSISHYDSQYAISEILNERISFGLVGSKINNPQIEYLDL
                     LDDELVLITPSDFKIDNKNNCIDIGELAYLNFIMRKEGSGTRNLILNTLSKNNFPVSK
                     LNVIAHVESNEAIKEMVRLGLGVSFISYISAIDYLNAGKIKCYKIKDVDFTRKFFFIY
                     SKKKTFSPLEDKFLNRLCEYFEIII"
     gene            15871..16605
                     /locus_tag="ACGI59_002479"
     CDS             15871..16605
                     /locus_tag="ACGI59_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9740262.1"
                     /translation="MKLKRSLVCVTILGIILVGCHKENTKEKNQVASKATQQKTMTKV
                     QNDVNEIMNKDYKYIIKNMGIPYNTFYYIKPKVLKESNTMQDINTSSYMTLVYPKYTG
                     NDELDGSALYVDINENKVVNVETNSFSSQGISVIDAESSIVIEKSDHEKSAVSLENFR
                     HIDLGEYVGVEDSRINEIVGDANYDLTAYNHEGSKVVKSYRLKEDNKILKKEVLTISI
                     VDNKIKSIKTIESDKIVKIIKGTLLE"
     gene            16816..17997
                     /locus_tag="ACGI59_002480"
     CDS             16816..17997
                     /locus_tag="ACGI59_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HFG9740263.1"
                     /translation="MIELEDAIKIIEKNVLPINRVKIVNLIDAVNKVVAEDIYSTIDS
                     PPFDRSPYDGYAYNANSENRNLKVIGTLYAGDVFNRVLNKNEAVKIMTGGKIPQGANC
                     VIKKEDVTVISDMITLNIKLKEYDNYIFKGEDIKKGQLIINKNQNIGYDGIGVLASLG
                     ISKVTVYDDLKVGILNTGTELQDINETLENGKIYDSNRYTLYSRLLKLGIQADTICNC
                     SDDILELEKKVKNMLQSVDFLITTGGVSVGDKDLIPDVFKKIGATELFWKINIKPGGA
                     CYVGTLGEKLLFGLSGNPHAAIVVFDNVIVPVIEYLTYKKGTHHIKAIFKGEFNKLSN
                     KDRFVSGKLYTSEGKLYVELNDKKDAKARLSATLRSNCMIKIKKNETIKENQLVDVVI
                     R"
     gene            17981..18553
                     /gene="mobB"
                     /locus_tag="ACGI59_002481"
     CDS             17981..18553
                     /gene="mobB"
                     /locus_tag="ACGI59_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HFG9740264.1"
                     /translation="MLLLDSFMKGHNFMYNILSVVSYSGVGKTTLIEGIIKELNKKGY
                     RVGVIKHTCHDFDMDEEGKDTYKHRKSGARKVCIISDKRVAYIEELKEKNPLYKMIQL
                     YEDMDLIIIEGYKNYRFKRLEVTRKGKYEDILSNKSDLIGIVSDIEYKLNIEQFNLND
                     YKNISKYIESCIKNEILSISNVEIKNILKE"
BASE COUNT         7341 a         2002 c         3232 g         5979 t
ORIGIN      
        1 agattgctgg agagacattg acataatgtc atggatacct ttcttaatat tattgctaat
       61 aacttgttca tgtggtggat tctttgatgg tgaagtaggt tgcggctgct aatacttaaa
      121 tcattagaat aaaaaaatgc tggtatttta taccagcatt tttttatttt attttttaat
      181 atgatttaaa ttaagaatga atattattaa atttgttgaa tatagggtat aatataaaat
      241 ataatgtatt taaatgtact tgcttttatg gtaaaataaa aacttatata aatgtagtta
      301 aaataggaac cttcaaaaat ttggttcagt tttagataaa tttagtaaaa taaggaggat
      361 gaagatgaaa ggcaatataa cgataaaaga tgttgctaaa caagcaggag tgtcaatatc
      421 tactgtatct agagttataa atgattcaaa acctgtaact gatgaagtca aacaaaaagt
      481 tttagaggtt ataaaagaga ctggatatat accaaatcca cttgctagaa gcttagtaac
      541 aaagaagagt caattaatag gggtaatagt tccagaagtt tcagattctt ttgttaatga
      601 ggtgttaaat gggatagaag aggttgctaa aatgtatgac tatgatattc ttttagcgaa
      661 tacatactct gataaggaac aagaacttaa gagtataaat ctattgagag caaaacaagt
      721 ggaaggtata gttatgattt catggatagt tgaacaagaa catatcaact atatacaaaa
      781 ttgtggaata ccagcgacat atataagtaa aactgctaga aattatgata tatatacagt
      841 aagtactagc aacgaagaag ctacttttga tatgacagag catcttataa agaaaggtca
      901 tgaaaagata gcttttataa tgacgagtaa agatgatact gttttagaaa tggaaagact
      961 tgctggttat gagaaagcac tttcaaataa caatatagaa ttagacaaga gtttgattaa
     1021 gtatggtgga actgattatg agagtggata caatagtatg aaagaactat tagatgatgg
     1081 aataatacct catgcggctt ttgtaacagg tgatgaggct gccataggtg ctataaatgc
     1141 tatatgtgat gctggatata aggttccaga agacatatct gttgcaggat ttaatgatgt
     1201 taagatagct agaatgtata gacctaaact tactacagta tatcaacctc tatacgatat
     1261 gggagcagta gcaataagaa tggttataaa gttaataaat aaggaattaa ttgagaataa
     1321 gaaaatagaa ttaccttata gaattgttga tagagaaagt gttacagaaa gaaaaaaata
     1381 aaactattaa aatcaatcag gtagtgccta aggcactacc tgatttttta tgtcaataat
     1441 atcaaattta ataatagatt aactaaaaat aattatctaa ttacttaaat agtttatttt
     1501 tatctattca tgagggttag actttttagt tttcttaaat aaacttttag attttttttg
     1561 ttgactacct ggtgtatttt ttggtgcagg tgaatttgac ttacctttag taggtttagc
     1621 cttcttagtt ttagctggtt tagtatctct ttggttttct gcatctgaga ttttttccat
     1681 tatattatcc agttttttgc gttgattttc atctaaaaat gctttgaatt ttgatactac
     1741 ttcatctctg ccatctaggt tcttacctat ttttataagt tcctccataa gttcattttc
     1801 tgattttcct ttgtaactat cagccatctt ttctatttta tccttgtcaa tatttttttg
     1861 tttagccatt ttttctaatg actctgtatc taattttttc ataaaaaaac ccctttctat
     1921 aaatatatat tgaattatat tcatactatt tatatataga aataatattg aagtggtgaa
     1981 ttgtgagtga tatataatat aaataaaaat tcttatagat aatatatatg cactgagctt
     2041 taaaaatgga acattaaaat atgaaaaaca tactttaata tagggcaatt atataatata
     2101 agggggaatt gtcttgaata acttactatt tttgattgga gtgattgcat tgagtaatgg
     2161 taatatctct atatttgatg ataaaaataa atcaaaagga aataatagag ttagaaaaaa
     2221 caaaaaaaat gcaaatgata aactttcaca gtctacacat ggcaatttaa ggtttgatgc
     2281 aaatgatata aaaagaggtt taaggctcat ggatttaagt gaagaagatt tagaaatggg
     2341 aatggaaata ataacaagaa ctaaaaaata catgtcaaga gatgaaagaa aaatactaat
     2401 aaaaatagaa agtgttttag atttagttag aggaataaaa aaattaaata atatagatat
     2461 ggtagataca gaagatgaaa cggatttttt cagaaaaatg gatagtgatg ataaaaagaa
     2521 tatgatgata aaggaaatta tagatgtatt cccagaaaaa agaaaaaatt cagttgaaaa
     2581 agctatagga atgaaaaaga agatagattt atttgcagaa ttatttttac cagatgactt
     2641 tggtgaaggt ggatttagtt tgagctcttt ggcaaatata aacaatcttg gtagtatgaa
     2701 taatctcaaa ttgttaggaa gtcttttaag aggtgatgat agtaataatg atgatgaaga
     2761 caataatgat gagttaaatt atgaagacga agaattagat tgtgaagaca aagaattgga
     2821 tgatgattat gatgagttaa cagacgaagg tgaaatggag gataatgaag atgaattagt
     2881 agaagataag aacacaggct atgaaaatat agaaaatgat gaagatatag tatatgatga
     2941 ggaactatac aacattaatg agcaagaatc cgaatacaat tataatgaaa aaaatactaa
     3001 aactagagct aagaaaaaac gataattaac taaatacata aagaaaaaag gaagaaaaag
     3061 taatgataga ataagattat ctcaattact atataaaacc actttaaaaa attcaaaatt
     3121 tattatataa taataatatg aaatcctgaa acatacgata aggctttttt aaattcaacc
     3181 tacaaatgaa atacgggaga ccgtgtctgg taaattataa catgcctagt tagtttggac
     3241 gttaaaggtt actggcaagt cacccacctg ggagtgccta gggtgtgaat ttacttagcc
     3301 accggtgttt cgggacaaaa aaagtgctac tatttcaaat agtagtactt tttttattaa
     3361 tatgcatttt tctactaaat acgcctaatt attgataaat tcaaaaagca agtaacaatt
     3421 aagattataa aagcatagta ttaattaggc ttcaaaaagc caaagactta ataagaatcg
     3481 cttacaggga gggcaatgaa aaatgcaaga ttataaaaaa aataaaagaa gaatgatgaa
     3541 tcagccaatg tctacaatga atgaagaaga agtgtataca gatgaaataa attcagaaga
     3601 catgagaggt tttaaaaaat cacaccatca taatggatgt aatactgata ataagtgtga
     3661 gtgccatgat gattgcaatc catgcaaccc atgtaatcca tgtaaaccta acccatgcaa
     3721 tccatgcaaa cctaatccat gtgatgacaa ttgtggatgc catgacaatt gtaaatgtga
     3781 ttgtgaacca tgtgaaatgg attcagatga atgttttgaa aacaaatgtg gaccagaatg
     3841 ctgtaatcct atatctccaa gaaacttctc tgtatcaaat gcagtgccat ttgcaataga
     3901 ggctaataga atatttgata ctatgcaatt ccaaacattt acagatgcaa caggaccaaa
     3961 tggagagcca ttaacttttg aaacagaagt agtagaagta tttggttcag ttccaagtgc
     4021 aggtcaagca agtgtaacta tagaaaaaat atgcttaagt aatgatggaa tcgttataga
     4081 cacaggaatg acaactttag aagatttcga tttagaccca ttaggagata tagtaggaag
     4141 aaactgtgaa acaacttttg aatttgcagt ttgtggagaa agaaacgctg agtgctgtag
     4201 acaaggaaaa ggcaaatcag tagcttataa acaaagagga ttaactgtag cagttcgtaa
     4261 tttagtacta gagctaagag gtagatgtgg atgtacagag ttcgttgcat tagctttccc
     4321 agcagttaga gcaggaggtg gatgtaagag aagagttgat tatgtagaat ttacttttaa
     4381 cacactttca gcaccaatat gcttgccagc tgacggaaga gctgttactt taagacaaga
     4441 atatcaaact aacttaactg tagattgtat aggaaaatct atattaaaat tagaatgcaa
     4501 cgaatgttgt gaacctttct atgaattaat tataccaaat gatatagatt tagtactttg
     4561 cttacaagaa acagttagca cattaataag tgaacaaata gtagttttag catcaccaaa
     4621 tccaatccaa ccaagacttg ttgatacttt ctctaaagta tgtgattttt cgcaatgtgg
     4681 acctaatcat ggaagtggaa agccaagttg ccacagatag ataggtaaga tatatagaca
     4741 gtttaaatta taaataaaaa tcatctaagg aaggctacca aagtgtataa tttacattgg
     4801 tagccttcac gtttttataa ctagcgtata gattttagtg atttaaaaag cactatagta
     4861 tgtaatagac aagtaaagat tgatactatc gtcattgtgt atatgtatgc ataaatatta
     4921 agtcctggaa ttggaagaaa tacatataaa gctattagtt gaattaacat gccagtaata
     4981 gtatttatac ttgaagctaa ttcttttctt attgaatgta aaatgccaga tagagtttga
     5041 tttagggaca taaatagtgg aactaaaaac atagctttta gatattctcc tgcaagtgta
     5101 tttttaaata ctaatataca taaatctttt ccaaagaaat aaaaaaatat tgatgatagt
     5161 atgcctacaa ataaagtaag aagtatggag tatttgattt ttttaattac actcttacgc
     5221 tttcttagag ccatttcttg tgaaatagtt ggtattaaat ttacaataag cgctgaacca
     5281 acagtaaaag gtaaaaatat aaaaggcatt accatcccac tgacaatacc atacatactt
     5341 aaagattgtt gataagtcat tcctgataat gctaatcttg atggtatcat catagaactt
     5401 atagattgca aaagtatgtt tgacattcta ttacaagtta tgggaataga catttttaat
     5461 gtttccatag aagaattata aaaatctttt atgcttataa tatacctatt gtcaaatgat
     5521 gaatctcgcc ataagcaaat aagcataaat ataatattac tcaactcacc gattgatata
     5581 cctaatagag ctatataaca attcatagat ttattattta tgtacataac tagtaaaaaa
     5641 acaaataata ttttaccaat ttgttcaatg atttgtccaa tagcagggat ttttacattc
     5701 tttatgccat agtaatatcc tcttagtacg tttgaaagag ttattataac tatagcagga
     5761 caaacagcta gtataaatag atttaattta gcatctttta aaaatttaag tgataaaaaa
     5821 gaactattga aagatgcaac tatagatata attagtgcta cgaaaaatga tatatataaa
     5881 gttgatataa ataaagcatt agtattgtgt ctatcgttta aagcttttct ctttgcaact
     5941 agacaactaa gtgcagtagg tatacctgtt gttgtaacag ctaagcaaat catcaaaaag
     6001 ttaaaaatca tatgatagat acctaaacct gtttcaccta gtgctcttga taagaatatt
     6061 ttatataaaa aaccaaatat tcttacaata aaattggata taaataaaat aactgttgat
     6121 agtactaaat aaggtacttt caaaaaaatc acctccttat cttttaaata tattcacaag
     6181 gaggtgtaaa aatttataat tttataaatt ctggtttcaa tttattaaat ttagatacat
     6241 atttaaatcc catatttttt agtaatgaat atatgtagtc aaactcacat gcaacttgac
     6301 ttatatgatg tgaatctgaa cctgtggtta tgatttcccc tcccaagtct ttatacattt
     6361 gaagtatata cttagatggg gttaggtctg gaagattata tctataacaa gaggtattta
     6421 tctcgatacc tttgccatca taaatagctt gtttaagagt ttcttcaatt atatcagaaa
     6481 atagtctatc atctaaaata gtgtcataag gtgcatacct tttaatcaaa tctaaatgac
     6541 ctaagacaga gtaatcttta taattcttaa caatattata taaataaaga tagtaatttt
     6601 cataaacttc atgttgagtt ttatctttaa aataattacc aaggtataag tccattgtat
     6661 ctatagcatg gatagaacat attataaagt ccaatggata ttgatgtgct tcctttttgt
     6721 atgtatccat taattgagtt tgaactccaa attcaattcc tttttttatt gatattttat
     6781 ctttatattt gctttgaaga gtctctaaac tcttaaaata atcttcgtag acaattgaaa
     6841 aagaatcatc agcatataca tcatagtcaa catggtctgt gaaacaaatt tcctccaaac
     6901 cgagttcaat agattttttt accatttctt ccattgtaga ttttccgtct gtagagaaac
     6961 ttgagtgcat gtgatagtcg taaaacataa atatttcccc tttttataaa aattttggta
     7021 ttatatatac atactaaata atatcaaata tacataaaag catcaataaa atataaatac
     7081 aatgagcggt gataaatttg gaaaaataca gaatagattt aaatgagaaa aaatcaacta
     7141 aatatataca gatatttaat catataaaag aattgataat aaataaagag ttagtagaac
     7201 atgaaaaatt gcctccaata agaaaactat caaatttttt acaagtaaat aacactacta
     7261 tagttaaggt atacgagctt ttagaaaatg aaggatatgt atataaaatt atagggagtg
     7321 gtacttttgt atcaagttta aattcttata aaagcggtat agataacagg aaaaataaga
     7381 ttattgtgca aagagaagat ttaattcatt ttgacaatgg gaatccatct aatgatatgt
     7441 ttcctatcga ttcatttaaa aatgctgtaa atatggcatt atctaaagat ggtagttcaa
     7501 tatttgaata tgatgaaggt cttggctctg aagaattaag agaaaaaatg gtagagtatc
     7561 taagtgatgg aaatataaaa actactaagg aaaatattca aataatatca ggtgctcaac
     7621 aaggtataga tatagtttgt aaaggactta taagttattc agatgttgtt tttatggaag
     7681 aaccttctta caatggagca attgaagtgt ttaaaagtag aggtgcaaaa ataatatcta
     7741 tacctatgtt agatgatgga attgatatag gtattttaaa attaaagttg gaaaaaataa
     7801 aaccaagact tatatacatt atgcctaatt ttcaaaatcc aactggaata tcttactcaa
     7861 catataaaaa aaagaaattg atagaattgg cagaagaaca tgatttttat ataatagaag
     7921 atgattttat aagtgatttt gtttttgaat caaaggacaa tagaacaatt agaagttatg
     7981 atgataaaaa tagagtagta tatataaaaa gcttttcaaa aatacttatg ccaggtctta
     8041 ggataggaat tgtagaaatg ccaaatgagt tgttaaaaaa agttttatgg gctaaatatt
     8101 catcagatat atcaacacca ggtttaattc aaaaatcaat gtattactat atgaataatt
     8161 ttgattggaa gaattatttg agtcatatag aaaagatata tactgataag tataaattgg
     8221 caaaaaactt gattaatgac aaattgagtg gtaaattaaa agttagaaaa tcttgtggag
     8281 gaataaactt ttttttagaa ttaccaagag gatatacatc tcaagcattt acaaatttta
     8341 tgttaaacaa aggagtgtca atgcttccag ggacttattt ttttgacaat ctagtggatg
     8401 atagattctt taggataaat atagctcgcc caagcatgga agaactagaa aagggaatat
     8461 ctataataag tgataatata gatgagtttt tttgtgaata taaaaacaag ttagatataa
     8521 aaagcaataa attattttat taaataaata gaggtttttt aggaggaaaa aatgtttgac
     8581 aagaagaaat tagataggat taatgaatta gcaaagaaaa ataaagaggg aatcctttct
     8641 gctgatgaaa tcaaagaaag agaaatttta aggaaagaat atttagaaaa ctttagagcc
     8701 cactttagat caagactaga tagtgtcaaa gtagtttcac cagaagaata tgagcaatac
     8761 atgaaaaaca ataaaaatta gaacttagta ggaggaaaat atgaacttaa agtacgaatt
     8821 taagaatgct tgggattttg aaagaaaaga aaatactatt gaacaaatta tgcaatattc
     8881 tagcaattat atggagtttt taagtaagtc aaagactgaa agactttcag taaaagaaat
     8941 tataaaattg gctaaagaaa ataactatat ttcaatagat gaagctatgg aaaaaggaag
     9001 cataagttgt ggtgataaga tttatgttat aaataaagag aaggcagtag cactttttgt
     9061 aataggtaaa aattatatag aaaaaggtat gaagattata ggaagccata tagattcgcc
     9121 aagattagat ttaaaaccaa atccactgta tcaagagagt aatctaggtt tttttaagac
     9181 tcattattat ggtggaataa aaaaatacca atggacggct attccacttg ctttacatgg
     9241 tatagttata ttaaatgatg gaacaaaagt ggacatatct attggggaag aagatagtga
     9301 cccagtattt tgtgtaactg atttattgat tcatttggct ggagaccaaa tgcaaaagaa
     9361 actatcagaa ggaattagtg gagaagcttt aaatatatta attggaaata tgcctttaga
     9421 agatgaagaa aaagaaccta taacagcaaa tatattaaag attttaaatg agaaatacaa
     9481 tatagttgaa gaagatttat taagtgctga aattgaagta gttccagctg gtaaggctag
     9541 agatttaggg cttgataggt ctatggtgtt gggatatggg catgatgata gggtatgttc
     9601 atatgctgct gtaaaagcta tactagaaac tgaacaacca gaatttactt cagttgcatt
     9661 atgtgtagat aaagaagaaa ttggttctaa agggaataca ggtatgcact caaagttttt
     9721 tgaaaatact gtggctgaac tgattgcttt agaaggagat tattgtgata ttaaagtaag
     9781 aagagcttta gcaaatagta aggtattatc agctgatgtt tctgctggat atgacccaaa
     9841 ttttggagaa gcatatgaaa agagaaattc ggcatatatg ggaaatggag tagttttaac
     9901 taagtataca ggttcaagag gaaagagtgg atgtaatgat gctaatgctg aatttatgtc
     9961 agaagtaaag agaattttta ataaaggaaa tgtaatatgg caaactgctg aacttggaaa
    10021 agtagaccaa ggaggaggcg gaactatagc ccatatattg gcaaatcaag gtgcagaggt
    10081 tatagactgt ggtgttggag ttttaaatat gcatgcacca catgagattg tctctaaagt
    10141 agacatatat gaaatgtata agggatataa ggcatttttt aatataaatt tataagtaaa
    10201 aatataaatg taataagtga ataaaggctg ttgtataaac aatttaatct gttgtacaac
    10261 agtcttattt ttatgtaaaa atagcaattt gtcacactag agaaaataaa agaaaatact
    10321 gtcaaattaa aaattattta aaaaaaaatt gacaacgatt tcatcttgtt ataatatgta
    10381 ataaagaact tgttatataa tgttataaaa atgttacata gggaggtgag caaaatggat
    10441 gaaaagagac aaaaaaaaga aatggattta gaagtaaatt cctttgttcc tttatatcaa
    10501 caactatatg acaacataaa aaaacaaata gcatctggca tatataaacc aggagataaa
    10561 cttccatctg aaggagactt atgtaaagaa tttaatataa gtcgtataac tgtgagaaat
    10621 gcacttaatg aacttgtaaa agaagatata ttatgtaaaa aacgtggtaa agggacttat
    10681 gtaacaatac cagagagaat agaagcaaca tgtgcaggaa atagttttac taattcttgt
    10741 catcgtatta atgcaaaacc aagtaccaaa ataatctctg ttttaataaa aaaagcagat
    10801 aagcaagtag ctgaagcttt aagtattgaa ctagaagaga aggtaatatg tattaaaaga
    10861 cttagattaa tagatgaagt acctgttatt tttgaagttg actattttag aatagattac
    10921 atgtttttat taaaagaaga attagaagat aaatctttaa tggaggttat atctaataat
    10981 ataagcacat tacctaaacg agtagaaaat atatttgaag taaaacattc aaacaaagaa
    11041 tattctgacc atcttaaatg tgcaagtaat atgccactat tgaaagtaag acaatcggtg
    11101 tatacagaaa ataatgatgt cttatattac aatgagcaat ttataagaag tgataagtac
    11161 aaatatgcag tatcagcaga aatataatct ataatttagt aggaggatga gtatgaaaaa
    11221 gataatatta gcatctcatg gagacttttc aaaagggctt ttaaatgcag taaaaatgat
    11281 agtaggagat ttagcagatt gtgtcagttc gtatggtttg taccctggac aaagtgcttc
    11341 agattttgct ttagaattag aaaaaacaat atcagaagat aaagaatgtg aatatataat
    11401 tttatctgac ttatatggag caagtgtatg tacagcaatg ttaagattaa ctaatctaca
    11461 taatgttagg ttattttctg gaatgaattt gaatatggta ttagaacttc ttacaaggtt
    11521 ttctgatgaa ttaacagttg aagatataga ccaattagtg gaagaatcta gaagaggaat
    11581 acaaagcgtg acattacaaa ttaaagaaga agaagaggta ttttagttta tgaaaaaaaa
    11641 gggaatatta tcagtattta aaagtaaaaa accaattata gcaatgatac acttaaaagg
    11701 agatactcca gaagatatct ttgaaagagc taaaaaagag attacaatct ttgaagaaaa
    11761 tggtgtagat ggaataatgt tagaaaatta ctatggtaat tattatgatt tagaaagaat
    11821 acttgaatat gtatcaaaag ctaatttatc aataccatat ggagtaaact gtttaaatgt
    11881 tgatactatg ggatttgaac tggcaacaaa atataatgct agttatatac aggttgattc
    11941 tgtagtaggt catgttaaac ctagagatga agctacttta gaagagtttt ttaaattaca
    12001 acgttcaaaa tgtccagcgt atttaattgg aggcgtacgt tttaaatatc agccagtatt
    12061 atcagaaaat gatgttgaag aagatttaaa aataggaatg acaagatgtg atgctatagc
    12121 agttacagaa aatgctacag gtcaagaaac atcaatggaa aaaatagaac tgtttcgtaa
    12181 aaatttagga gacttcccat tggttatagc tgctggagtt acattagaaa atgcaaaaaa
    12241 acagcttaaa ttaggagata tggcaataat aggaagttac tttaaagata attacaaaga
    12301 ttttggggat gttagtgtag aacacgtaaa aacattcatg gatgaaatca aaaaaattag
    12361 ggaggaatta taatgattaa attagtaaga gtagaccaca gacttataca tggacaagta
    12421 gcatttacat ggacaaagtt tttaagtaca gactgtatat tgatagcaag tgatgactta
    12481 ttaaaagatg aattaagaat ggcaggactt aaaatggcta aaccatctaa tgttaagtta
    12541 gtaatgaaaa gcatagcaga ttctataaaa gcacttaact caggtgttac tgataaatac
    12601 aacttattga tactttgtga atctgtagaa gatgtttaca gacttgctaa agaggtaaaa
    12661 gctattaaat ctataaacct tggtggaaca aaatcagatg ataatcgtga aaatatatct
    12721 aaagcagtgc atgtatcaaa agatgatata aagatgatta aagagttaga ttcagaaggc
    12781 gtaaatgtat ttgtacaatt agttcctgat gatgatgcta caaatgtaat gaaattaata
    12841 taattttagg gggatgaaaa tggaatttac acaagttatt ttgataactc taattgcatt
    12901 ctttgcatat atgcatagtt ttgtaggttc tacaatgcat aatagaccta tagttgttgc
    12961 accacttgtt ggtttagcac taggtaatct tcatacaggt atagtaatag gttcaacatt
    13021 agaattagta tttatgggtg catttcctgt tggggcaagt aatcctcctg attttgtgtc
    13081 tggttctatt attgccacag cgtttgtaat attaacgggt caagatgtat cagcagcagt
    13141 tgtacttgca gttcctatag caactctagt attgcttata gataacttct taatgacagt
    13201 agtacttaca tggggagctc atattgctga ccgttatgca gaagagggga atattgaagg
    13261 cgtagaacgt gtgcaattat tatttggtat aggaaataaa ttaatattag caataatagt
    13321 aggtatagga ttttcattag gtgttccagt aatagaaaaa atactttcat ttataccatc
    13381 atatgtaact catggaatgg atgttgcagc aggcgtgatt ccagcaatag gttttgccat
    13441 gttagctaga atgatgctta ataagaaaac agtagcattt ttattattag gatttatatt
    13501 ggttgcttac ctaaacataa cagtaacagg agttgcattg tttggattag caatagcttt
    13561 aatatatgtt aactttgtag gagagaagga ggtaatagta gatgacaacg agttctaaga
    13621 aattagagac aatttcacca gatagtaaaa taacgaggaa agacttttgg aaatgtttta
    13681 gaaggtcatt gacattagat tcatcttgga actacgaacg tatgcaaaat atagcatatg
    13741 catacatgat ggcaccaata atccgtagat tatataaaga tgataaagaa aagaaatcta
    13801 aggctttaaa aagacattta gaatttatgt cagttactcc tcatataagt actcttttgg
    13861 taggtatatc tggagctatg gaagaagaaa atgcaaagaa taaagaattt gatgctaata
    13921 gtataaatgc tgttaaatca agtttaatgg gtccagtttc tgggatagga gattcatttt
    13981 tctggggaac attaaagctt atagcagctg gggttggtat agctttagct tctcagggta
    14041 acataatggg accgatttta ttccttttaa taataaatgt tcctcatttt attatacgct
    14101 atatatgtct agataaagga tttaagtatg gaactcaatt ctttaaagat gtgagtggtt
    14161 caagtatagt ttcaaaagtt atggaagcag cttcaatgtt agggcttatg gtaattggag
    14221 gtatgactgc atctaatgta atgttaaaac ttagtgttaa tgtaggtagt ggagaatggg
    14281 cagagcctat tcaaacttac ttagaccaaa taatgccatg tatgcttcca gctatgatat
    14341 tcggtattat gtattggtta ttaggaaaaa aggtaaagac aactacgata ttaatttcag
    14401 ttatgattat ttgtatagta ttggctgcta ttggagtagt ataaataaaa aaatcccaag
    14461 ttcatttgaa cgagggattt tttgactaaa ttaaataaaa aagaaagatg gttcataata
    14521 atttataagc atttatgtga tagttataat tagcttatta attagttata aactcaaaat
    14581 taagcactac tttattaaag ttattatatt ataatttcga aatactcaca taatctgttt
    14641 agaaatttat cttctaatgg ggaaaaagtt ttctttttag agtaaatgaa gaaaaacttt
    14701 ctagtaaaat caacatcctt tattttataa cattttattt tacctgcatt gagataatcg
    14761 atagctgaaa tgtacgatat aaaggacact ccaagaccta atctaaccat ttccttaata
    14821 gcctcatttg attctacatg agcgattaca tttaatttac ttactggaaa gttattttta
    14881 ctaagtgtgt ttagtattaa gtttcttgta cctgaccctt cttttctcat aataaaattt
    14941 agataagcca attctcctat atcgatacaa ttatttttat tatcaatttt aaaatctgaa
    15001 ggagtgataa gaacaagttc atcatctaat aagtctaggt actctatttg aggattattg
    15061 attttagaac caactaatcc aaagcttatt cgttcattta aaatttctga tatagcatat
    15121 tgagaatcat aatggcttat ggaaaatttt acatctggat aactcataga aaaactcttc
    15181 ataaaatcag gtaaaatata agtttcaggt atggaactac atgctatatc tataatacct
    15241 tctatttttc ctgaatattc ttttatatca taaattgcac gtttgcaatt attcaatata
    15301 tatatagcat gttcataaag aatttcacca gattttgtga gtgtaataac tttgttgctt
    15361 ctatttataa gaacagtttc tagctctctc tctaaattct gtatgtgtgc gcttacagta
    15421 ggttgagtta aaaaaagttc tcttgcagcc tttgaaaaac tctgatgttt tgcaacagca
    15481 acaaatactt caagttgttt aaaatccatt atttaatcct ccttaaatta tctaaaacta
    15541 ttataacata tttgataaat taatagtcaa aggtgatttt taaaatagaa aacctttaaa
    15601 tattcatgga gtttttgatt gtttaggaat aatttggtat aatgttgtaa ggtacttaca
    15661 ataaaaatat cgtaaattga attttcaaaa ttcaacttta aagtgttttt tattacagaa
    15721 aaattacaat tagcttctta atcttattaa attcaaataa atattatata attattttat
    15781 aatataataa atataattat tgaaaaaaga gataactgta aattgagata tagataaata
    15841 ctataaaatt taagaaagga ggattgattt atgaagttaa aaaggtcatt agtttgtgta
    15901 actattttag gtattatact tgttggatgt cataaagaaa atactaaaga gaaaaatcaa
    15961 gtagcaagta aagcaacaca gcaaaaaact atgacaaaag ttcaaaatga tgttaatgaa
    16021 attatgaata aagattataa gtacattata aagaatatgg gtatacctta taatacattt
    16081 tattatataa aacctaaagt tttaaaagaa tcaaatacaa tgcaagatat aaatacatca
    16141 agttatatga cattagttta tccaaaatat actggaaatg atgaactaga tggaagtgct
    16201 ttatatgttg atattaacga aaataaagtt gtcaatgttg aaaccaattc tttctcatca
    16261 caaggtatat cagtcattga tgctgaatct agtatagtga ttgaaaaaag tgaccatgaa
    16321 aaatcggctg tatcactaga aaattttaga catattgacc tgggagagta tgttggtgtt
    16381 gaagattcca gaataaatga aattgtagga gatgcgaatt atgatttaac agcatataat
    16441 catgaaggca gtaaagtagt taagagttat agattgaaag aagataacaa aatattaaaa
    16501 aaagaggtac ttacaataag tattgtggat aacaaaataa aatctataaa aactattgaa
    16561 agtgataaaa ttgtaaaaat aataaaagga actttattag aataaaatat tctaaagcat
    16621 atgatgctgt ttcaagatag aaggaaattt ctagctggga acagtttttt tattagattt
    16681 ataaaatttg aactgcactt atagataact attagttata attaaatata agataaaagt
    16741 atttccaact tttataatct ataatatatt acatagaaat atcttaaata aaaaatatat
    16801 tggggagtat ttaaaatgat agaattagaa gatgccataa aaattattga aaaaaatgtt
    16861 ttaccaataa acagggtcaa gatagtaaat cttatagatg cagtaaataa ggtagttgca
    16921 gaagatatat attcaacaat tgatagtcct ccatttgata ggtcacccta tgatggttat
    16981 gcatataatg caaattctga aaatagaaat ttaaaggtta tagggacttt gtatgcagga
    17041 gatgtattta atagggtatt aaataaaaat gaagcagtga agataatgac tggtggaaag
    17101 ataccacagg gtgcaaattg tgtaataaaa aaagaagatg taacagttat aagtgatatg
    17161 ataactttaa atataaaatt aaaggaatac gataattaca tatttaaggg tgaggatata
    17221 aaaaaggggc aattgattat taataaaaat cagaatatag gatatgatgg tattggagta
    17281 ttagcaagtc ttggaatatc caaagtaact gtttatgatg atttaaaagt tggaattttg
    17341 aatacaggta cagaactaca agatataaat gagactttag agaatggtaa aatatacgat
    17401 agtaatagat ataccttata ttcaagatta ttaaaacttg gaatacaagc agatacaata
    17461 tgcaattgtt ctgatgatat attggagtta gaaaaaaagg taaagaatat gttacagagt
    17521 gtagattttt taattaccac aggtggtgtt tctgttggag ataaagattt aattccagat
    17581 gtattcaaaa aaataggggc aacagaatta ttttggaaga taaacattaa acctggtgga
    17641 gcttgttatg taggtacatt gggagaaaag ctattatttg gtctttctgg taatccacat
    17701 gcagcaatag tggtatttga taatgtgata gttcctgtta ttgagtattt aacatataag
    17761 aagggaacac atcatataaa agctatattt aaaggagaat ttaataaact ttcaaataaa
    17821 gatagatttg taagtggaaa actatataca agtgaaggta aattgtatgt agagctaaat
    17881 gataagaaag atgcaaaagc tagattatct gcaactctaa gaagtaattg tatgattaag
    17941 attaaaaaga atgagacaat aaaagaaaat caattagtgg atgttgttat tagatagttt
    18001 tatgaaagga cacaatttta tgtataatat attgtcagtt gtctcatatt caggagtagg
    18061 taaaactaca ttgattgaag gcataataaa agaattaaat aaaaaagggt atcgtgttgg
    18121 agttattaaa catacctgtc atgattttga tatggatgag gaaggaaaag atacttataa
    18181 acatagaaaa tctggggcga gaaaagtctg tataatatca gataaacgag tagcatatat
    18241 tgaagaacta aaagagaaga atccattata taaaatgatt caattatatg aagatatgga
    18301 cttgattatc atagaaggat ataaaaatta tagatttaaa agattggaag taactagaaa
    18361 aggtaagtat gaggatattt tatctaataa gtctgatttg ataggtattg tcagtgatat
    18421 agaatataaa ttgaatattg aacagtttaa tttaaatgat tataaaaata tatcaaagta
    18481 catagagtct tgtataaaaa atgaaatttt aagtataagc aatgttgaaa taaaaaacat
    18541 attaaaagaa taat
//
LOCUS       DAYAPA010000078        18485 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.078, whole genome shotgun sequence.
ACCESSION   DAYAPA010000078 DAYAPA010000000
VERSION     DAYAPA010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18485
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.078"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(<1..352)
                     /locus_tag="ACGI59_002482"
     CDS             complement(<1..352)
                     /locus_tag="ACGI59_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFG9740265.1"
                     /translation="MAALKSFEKPLTPEEEIEYLTKFKIENDKSAKDTLIERNMRLVA
                     YIAKKYNNSTEDQDDLISIGTIGLIKAIETYNIDKGTRLATYASRCIENEILMNIRSN
                     KKNKTQVSLQDPIGT"
     gene            919..1281
                     /locus_tag="ACGI59_002483"
     CDS             919..1281
                     /locus_tag="ACGI59_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9740266.1"
                     /translation="MKIRKSGFISIECIISIAILYVAVYLVSTSLYNCYSFISRNISD
                     REMLSTAKKYIEDEKYRIQNSKYELIEDKIEKNYINGYEINSKIEQILDYYQCYEINI
                     EIKNEFKKLRFNSYVTRK"
     gene            1265..1990
                     /locus_tag="ACGI59_002484"
     CDS             1265..1990
                     /locus_tag="ACGI59_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9740267.1"
                     /translation="MLLENKGSILIITLIIFSIISTVCIMCIGLIYTHNNMFELGYKD
                     IQLKEISLSGIEISVSNVLSCVEEAIDVSNNEDEFREYFLGNNMIECIKKIKDTSYSS
                     LEELSLKVENNTIYDRGDFFEFDIESNVREKEFLKAIKVRVKIKNPWIGIDIEDYMDK
                     SNNNKIDDKTFNNYKASENNEILNQFKNESENIRNQEVGGFTEEDEEDEEYEEELKQN
                     IIENIKKNFDEKNLVQVYKYRGV"
     gene            2010..2429
                     /locus_tag="ACGI59_002485"
     CDS             2010..2429
                     /locus_tag="ACGI59_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9740268.1"
                     /translation="MTVIELIVSISIILLIVTLSFPKDNLENHKINLFARQLCSDIRY
                     VRRINMFSNYNTYIYYINQGKHEGYVLRKDGKNIKSIMLPKNAKIARSIEIIKFKSDG
                     SPQKASTIEIYNNRLRKTITITPVSGRVLLKEGKYET"
     gene            2419..2943
                     /locus_tag="ACGI59_002486"
     CDS             2419..2943
                     /locus_tag="ACGI59_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9740269.1"
                     /translation="MKHKYGYLLLESVVSLSSMVIIILVLYSIFLSTINLKLKVEDKI
                     ELQQQSLEIIKSMEGIISNSMGIMNVSNYEETFKKTTSIKCRYLDENNNEEGISNKEI
                     ILNERRNKLFVNSLNGESSQAGGYEIGDYVDEMYVSITNNGQYVNIKLKLSKRSQKYE
                     TDFKIKVWNFSESI"
     gene            3255..3812
                     /gene="efp"
                     /locus_tag="ACGI59_002487"
     CDS             3255..3812
                     /gene="efp"
                     /locus_tag="ACGI59_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFG9740270.1"
                     /translation="MVSAGDFRKGVTFEKDGQPCLVVDFQHVKPGKGAAFVRTKYKNL
                     KTGAIREESFNPSEKFPKAVIDTRQMQYLYNDGELYYFMDQENFEQIPLNYEQVEDAI
                     KFLKENEVATIRFYQGQPFQVEAPNFAELEVTDTEPGIKGDTASNVTKAATVETGAVV
                     QVPLFINTGDKVKIDTRTGEYLSRV"
     gene            3904..4359
                     /locus_tag="ACGI59_002488"
     CDS             3904..4359
                     /locus_tag="ACGI59_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD1247 N-terminal domain-containing protein"
                     /protein_id="HFG9740271.1"
                     /translation="MKHLYEEVAYLKGLAEGLEISAESKEGKMIHKIVDALEVFADAI
                     VTLDEEQEELQDFVESIDEDLADLEEDIADMEEDLYEEDDDEDDEDFSYIEMECPNCG
                     ELVEIDEDLLYDDEVDVVCPDCKAVILSSEDDCDDDCTCGGCSSCDDRE"
     gene            4522..5232
                     /gene="rnc"
                     /locus_tag="ACGI59_002489"
     CDS             4522..5232
                     /gene="rnc"
                     /locus_tag="ACGI59_002489"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFG9740272.1"
                     /translation="MKISKKLLDNIQRFENVINYKFKNKEYILEALTHSSYSNENKKY
                     NFNERLEFLGDSVLGIVISDYLFNEEANLPEGELTKLRANIVCEDSLSEVANDINLGI
                     HMLLGRGEEATGGRHRTSILADAFEAVIAAIYLDGGFESARQFILHHMENIIYDSRKG
                     NIFRDYKTHLQEVLQGNGENNIWYRLIEEKGPDHNKRFVMEVGINDDVLGIGEGKSKK
                     EAEQLAAKIALKKKLWEK"
     gene            5286..6356
                     /locus_tag="ACGI59_002490"
     CDS             5286..6356
                     /locus_tag="ACGI59_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006448 - regulation of translational
                     elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongator complex protein 3"
                     /protein_id="HFG9740273.1"
                     /translation="MKKRIIPIFVPHKGCPHDCIFCNQKKITGVSTDVTSEDARNIIE
                     ECLETIDKDADVEIAFFGGSFTAIDIDIQKSLLSVAKEYVEKGLVKDIRMSTRPDCID
                     EDILSMLKEYKTSIIELGVQSLDEKVLLDSIRGHQSEIVYKSSKMIKNSGIKLGLQMM
                     IGLPADTEEKCIFTAKKFVELKPDCVRVYPTLVVKDTGLEKLMEQNKYNPFTLEESVN
                     IVKKVLVLFYVNNINVIRVGLQATDDIQIGKAVLAGPYHPAFRELVEADMIKDYLEFV
                     ILQNKNIKQMLVKSNKKNISKIIGNKKTNVKYMEEKFGVLLKTQESDLDINQLEIVLD
                     GKSLISANMRDIHRKLYDIYDL"
     gene            6384..9938
                     /gene="smc"
                     /locus_tag="ACGI59_002491"
     CDS             6384..9938
                     /gene="smc"
                     /locus_tag="ACGI59_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HFG9740274.1"
                     /translation="MYLKRLELKGFKSFPVKTDIIFKEGITAIVGPNGSGKSNISDAV
                     RWVLGEQSIKSLRGDKLEDVIFAGTDTKKPMNYCEVALTIDNSENQLELDFTEVTIRR
                     RAYRNGESEFFLNNKSCRLKDIKEVFLDTGIGKDGYSIIEQGKVDEILSNNPLSRRKV
                     FDEACGISKYRYKKQEAERNLSNTKENLERIDDVYIEIENQLKPLFNQQTKAKKYLEI
                     SEKLKTLEVNSFIREIEGIEKELSEVNEHRKVIEKELNEKEEQKNVVEKKQEDINKEV
                     EVLQDVIEKSVDYINSIKGVISKKESQINLIKERIRNFTNEISRKNLEIKDIKEKLNE
                     NKQYIKELESNKLSGSEELSTLQENIKVLEGSKDKQKIKLESLNNEIELLKESIIDIL
                     NKKQEFSNKLSTLNANKENMNIRDENINSEITELNKNIEIKSSELDTINKEFNMQNEN
                     LKNVNNRHKELSINLQDSISEHNKLEDEIQKSKYNLNGYNSKLNVYIDMENHYEGFNR
                     GVKEVLKNKNLKGVHGALGQIINVPEKYEKSIEAALGAYMQNIITDNEFSAKFAINYL
                     KQNNLGRVTFLPLNIIKSNKISLGNLKANTKFIGIASDLITFDEKYRNIIENILGRTI
                     LINNIDEGIKFAKETGHRFKIVTLDGEILNPGGSLTGGSLKTNGNILSRKRYINEYTE
                     KISNIKNEISHLELKRESLDKDVKNIKNEIDSHESKIKDLEKSIIIKSTSIKNVESEI
                     ESLKGSITKLENEKNDLNSNLNYTLEKSDDVRKDMEELDDLYNKNKEKIDALNEEIKR
                     YNDLYDKEKSEFDELNLSLVKKTEVYNSIVRDIKRISGENCELEEKNKQLEESLNYEE
                     HEIIKLQDSILTEEKEKENLTKQLGDSNRNLETRKIAKDDLKNSFDEINKELKTIDRQ
                     HIELKESLFKVGGRLERLKTSQDTYINKLFEQYDMTLVQALEIKDEDLDIDRKFLESL
                     KREIRSLGNINIDSIKEYEEIKERYDFYSEQKQDLEESMEEIEKLIHTLEENMKSEFE
                     IKFEEISKNFKYVYKRLFGGGCGELTILDKENLLESDILITAQPPGKKMKNLNLLSGG
                     EKALTAISILFAILITKPTPFCILDEIEAPLDDANIFRFGEFLKDLSKETQFISVTHR
                     RGTMEAADYIYGVTMQEKAISKVISLKLKEAQEITDII"
     gene            9965..11245
                     /gene="ftsY"
                     /locus_tag="ACGI59_002492"
     CDS             9965..11245
                     /gene="ftsY"
                     /locus_tag="ACGI59_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFG9740275.1"
                     /translation="MLKKLFGFGKDKEKEIEKKDAEEEIEVEDSVDNLENLEETIFSG
                     LEEEVIDKVEDIEEKNEESVNNEGTEEIENFEKIEVEMDSNGKEVENISNNDSQEEII
                     EELENYDEIESKEIEDKEDKKVNLFERLKQGLTKAKQGITDRIDEVLKSYTKIDEELL
                     EDLEEILITADVGVNTTMDIIERLRDKIKQKGITEPIKVREELKSIVEDILTNENSTL
                     DIEPAPCIILMVGVNGVGKTTTIGKLANRYKKDGKKVLLAAADTFRAAATEQLEIWAN
                     RTNVDIIKHQEGADPGAVVFDAIKAAKARKTDVLICDTAGRLHNKANLMNELGKVFKI
                     VDREFPEAKREVLLVVDATTGQNAVVQAKTFKEVADITGIVLTKLDGTAKGGVVLAVK
                     SEVDVPVKLIGVGESVEDLQDFNAKSFSDALFGN"
     gene            11348..11716
                     /gene="ylxM"
                     /locus_tag="ACGI59_002493"
     CDS             11348..11716
                     /gene="ylxM"
                     /locus_tag="ACGI59_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048500 - signal recognition particle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlxM family DNA-binding protein"
                     /protein_id="HFG9740276.1"
                     /translation="MNIEKMVEIGLLFEQYKELLTDKQKEIVALYYEEDYSLGEISEN
                     LNVSRQGVYDTLKRSEKILRDYEEKLHLVSKIQEQEKNIKIIKDKIIDIKEDLLHNRD
                     CANLIPKLENIEDVCREMIK"
     gene            11713..13065
                     /gene="ffh"
                     /locus_tag="ACGI59_002494"
     CDS             11713..13065
                     /gene="ffh"
                     /locus_tag="ACGI59_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFG9740277.1"
                     /translation="MIFEGLSDKLQGAFSKLKSKGKLTEVDVKNAMREVKLALLEADV
                     NFKVVKDFIKKVQERCVGQEVMQSLTPAQHVIKIVNEELTSLMGDVQSKVMISSKPPT
                     ILMMVGLQGAGKTTTAGKLGGYFKKQGKKPLLVACDIYRPAAIKQLQVVGEKLDIPVF
                     NMGDKENPINIAKAGLSHAIKNANDLVIIDTAGRLHIDEVLMDELKSIKSEVKPHEIL
                     LVVDSMTGQDAVNVAESFNEALGVDGVVLTKLDGDTRGGAALSIRAVTQKPIKFMGMG
                     EKLDDIEPFHPDRMASRILGMGDVLSLIEKAQENIDLEKAKELEQKIKKQELDFEDFL
                     QQMEQIQNMGPLDKILGMIPGMGNVKDQLGDVDLNGKEMKRTKAIIQSMTTYERRNPG
                     VLNASRKKRIAKGSGTSVQDVNRLIKQLNEMKKMMKMFAGSQKSMKKRGGLGGLPFFK
                     "
     gene            13103..13375
                     /gene="rpsP"
                     /locus_tag="ACGI59_002495"
     CDS             13103..13375
                     /gene="rpsP"
                     /locus_tag="ACGI59_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFG9740278.1"
                     /translation="MAVKIRLKRMGANKKPFYRIVVADSRAPRDGKFIEEIGYYNPIS
                     EPKQVRINDEKAIKWLATGAQPTEVVKKLLVKNGVIEKFEASKQAK"
     gene            13440..13667
                     /locus_tag="ACGI59_002496"
     CDS             13440..13667
                     /locus_tag="ACGI59_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HFG9740279.1"
                     /translation="MKELVVDIAKALVDNPDSVVVEEFEDNDGIVLKLTVAQEDMGKV
                     IGKQGRIAKAIRTVVRSVANRENIKVSLEIV"
     gene            13775..14290
                     /gene="rimM"
                     /locus_tag="ACGI59_002497"
     CDS             13775..14290
                     /gene="rimM"
                     /locus_tag="ACGI59_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438220.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFG9740280.1"
                     /translation="MKEKLTHFKVGQIVNTQGLKGEVRVYPLTDDIERFDELKDFYLG
                     KDLNNKLAVEKVRYKGSMVIMKIKGIDSIEKAEKLKNKFMYVAREDSRELEEGEFFIA
                     DMIGMEVLTVDGKYVGILEDVLQYSANDVYVVKGEEDKEFMIPAIKKFVPTIDIDERK
                     MIIDPIKGMID"
     gene            14299..14994
                     /gene="trmD"
                     /locus_tag="ACGI59_002498"
     CDS             14299..14994
                     /gene="trmD"
                     /locus_tag="ACGI59_002498"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFG9740281.1"
                     /translation="MRFHIMTLFPEIFNSYMDESIMKRAVEKGIIEVHIYNIRDFSNN
                     KHKKVDDYPFGGGAGMVMTPQPIYDTYKHIITTHNINNPSVIYLTPKGKVYNQSMAKQ
                     MSLKEDIILLCGHYEGIDERIIDLIVTDEISIGDYVLTGGELPALIMIDSISRLIPGV
                     LNQEESFEEESFKDNLLEYPHYTRPRDFEGLKVPEVLLSGNHKKIDEWRREESIRITK
                     ERRFDLYKKSNEK"
     gene            15179..15529
                     /gene="rplS"
                     /locus_tag="ACGI59_002499"
     CDS             15179..15529
                     /gene="rplS"
                     /locus_tag="ACGI59_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFG9740282.1"
                     /translation="MNEMLRAIEQEQLKNEVPNFGPGDTVKVHVRIIEGKRERIQVFE
                     GVVLKRQGGGARETFTVRKMSFNVGVERTFPVHSPKIEKIEVTRKGKVRRAKLNYLRG
                     RVGKAAKIKEARNK"
     gene            15850..16746
                     /gene="ylqF"
                     /locus_tag="ACGI59_002500"
     CDS             15850..16746
                     /gene="ylqF"
                     /locus_tag="ACGI59_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HFG9740283.1"
                     /translation="MSNEYEEYLMNDNLHINWYPGHMKKTKELVKNNLKLIDVVIELL
                     DARIPFSSKNPDIDRLVGDKPRVVVLNKSDMADRDKLNQWIEYYKKINIKAIPVDTIK
                     GVGINKIIEECKNVTREKMSSLKDKGRKERAIRIMIVGVPNVGKSSLINKLTGRKSTQ
                     TGDKPGVTKGKQWVRLKGNLELLDTPGILWPKFEDQEVALNLAFSRAIKDEILDTETL
                     ALRLIEKLMKIEPEKLKARYKLDCLGETPIETMDMIGHKRGFITGKKELDYTRIATTV
                     LNEFRDGKIGNITLEVPENVKR"
     gene            17135..18427
                     /gene="brnQ"
                     /locus_tag="ACGI59_002501"
     CDS             17135..18427
                     /gene="brnQ"
                     /locus_tag="ACGI59_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFG9740284.1"
                     /translation="MGKTKDVIVFGFALFAMFFGAGNLIFPPYLGIITGPEWLIAFLG
                     FTFADAGLALLAVMATAKFDGNVVEMFKRCGMKLGILIGCADILCIGPFLAIPRTGAT
                     TYEMGIMPLFGTSIPVLLFCILFFAISYVLTIRPSKVVDIVGQFLTPALLIALAFIII
                     KGIISPLGDIVDKPMIPNVFAEGIGQGYQTMDAFAAIALASVLIVSLNDKGYSTISDK
                     LKMIGKAGVLACGGLALVYGGLCFLGATVSTMYGTDAVQSQVIVNITEGLLGNVGKAI
                     LAVVVSLACLTTSIGLTSATGQYFSRLTKGKLSYEKIVLAVSVFSAVVASFGVGTIIK
                     IASPILSIVYPPSIVLIILAFFNEKIKNDNVYKGAVYMSLFVSILTVISSYGVAVPVV
                     NSLPLNSLGFNWVVPVIIAGIIGNFIPSKSQSNTLGTN"
BASE COUNT         7821 a         1728 c         3432 g         5504 t
ORIGIN      
        1 ctgttccgat agggtcttgt agagatactt gagttttatt ttttttgtta gatctaatat
       61 tcattaaaat ctcattttca atacatctag aagcatatgt tgctaatcga gttcctttat
      121 ctatgttata agtttctata gctttaatta atcctattgt acctatggat attaaatcat
      181 cctgatcttc cgttgaatta ttatatttct ttgctatata agctactaat ctcatgtttc
      241 tttcaattaa tgtatctttt gcacttttat cgttttctat tttaaattta gttaaatact
      301 caatctcttc ttcaggggtt aagggttttt caaaagattt aagagctgcc aataggtcac
      361 ccccttttca acggtcactt taaattatat gaaggagact tatccaagtt gactgttatc
      421 taataaaata atatgcatat ttttagaatt taaaacatat atttgcaaac ttttgaatgg
      481 aatatgacat aaagagtggg aatttaaatt taagtttttg ttgtatatat atgaaataag
      541 gcgaagaaat tattgattta taaagtataa attggaattg aggtgtcaat aaatataaaa
      601 ggaaatagta tttatatatg ttttggtgtt gaagtaaaag attaatgcaa agcattagag
      661 tctataaaaa tatgaatata ggaggtgtaa ttttacaata cttaaaaaag taaatgtaat
      721 taattgtttg tataaagttg attacaagca aaagtatcag tcatagttaa tactgttaag
      781 ggtgaaggtg gtgtgttaat ttatatatgt atgggaagaa attttgatta caatgaaaaa
      841 acttacaaaa gatatttatg aaaaaactta tagttaaatg tttatatata aaatattatt
      901 taggggttgt ttttattatt gaaaataaga aagagtggtt ttatatcaat tgaatgtata
      961 ataagtattg ctatattata tgtggctgtt tatttagttt ctacatcatt gtataattgt
     1021 tatagtttta tcagtagaaa tatatctgac agagaaatgt taagtacagc aaaaaaatat
     1081 atagaagatg agaagtatag aatacaaaat agtaagtatg agttaattga agataagata
     1141 gaaaaaaatt acataaatgg atatgaaatt aacagtaaaa tagagcaaat tttagattat
     1201 tatcaatgct atgaaataaa tatagagata aaaaatgaat ttaaaaaact gaggtttaat
     1261 agctatgtta ctagaaaata aaggctctat attaataata acattgataa tattttctat
     1321 tataagtact gtgtgcatta tgtgtatagg tttaatttat acacataata atatgtttga
     1381 gttaggatac aaggacattc aactaaaaga aatttcttta agtggaatag aaatatcagt
     1441 aagtaatgta ctttcttgtg ttgaggaagc tattgatgta tctaataatg aagacgaatt
     1501 tagagaatat ttcttaggaa ataatatgat tgaatgtata aaaaaaataa aagatacatc
     1561 ttatagtagt ttagaagaac tttctttaaa agtagaaaat aatactattt atgacagagg
     1621 agattttttt gaatttgata tagaaagtaa tgttagagaa aaagaatttt taaaagcaat
     1681 aaaagtaaga gtgaaaatca aaaatccatg gataggaatt gacattgaag attatatgga
     1741 taaatctaat aacaataaaa ttgatgacaa aacattcaat aattataaag catctgaaaa
     1801 taatgaaatt ctaaatcaat ttaaaaatga aagtgaaaat ataaggaatc aagaggttgg
     1861 tggatttaca gaagaagatg aagaagatga agaatatgaa gaagaactta aacaaaacat
     1921 aattgaaaat ataaagaaga attttgatga aaaaaaccta gtacaggtat ataagtatag
     1981 gggtgtataa ctttgaaaaa agaaggaatg tgactgtaat agaattaata gtatctatca
     2041 gtataatact cttgatagtt acattatctt ttccaaaaga taatcttgaa aatcataaaa
     2101 taaatttatt tgcacgtcaa ctttgttcag atataaggta tgttaggcga attaatatgt
     2161 ttagcaacta caatacatat atatattata taaatcaagg taaacacgaa ggttatgtat
     2221 tgagaaaaga tggtaaaaac attaaatcaa taatgctacc taaaaatgca aaaatagcta
     2281 ggagtattga aataataaag tttaaatctg atgggtcgcc tcaaaaggcg tctacaatag
     2341 agatatataa taatagatta agaaaaacta taacaataac acctgtaagt ggaagagtac
     2401 ttctgaaaga gggaaaatat gaaacataaa tatggttact tgcttttgga aagtgtcgta
     2461 tcattgtctt caatggtgat aattatacta gttttatatt ctatattttt gtctacaata
     2521 aacttaaagt taaaagtaga agataagata gaactacaac aacaaagtct tgaaatcata
     2581 aaatctatgg aaggaattat tagcaattct atgggaataa tgaatgtatc taattatgaa
     2641 gaaacattta aaaagacaac atctataaaa tgtagatatt tagatgaaaa taataatgaa
     2701 gaaggcataa gtaataaaga aattatattg aatgaaagac gaaataagtt atttgtaaat
     2761 tctttaaatg gcgaaagtag tcaagcaggt ggttatgaaa ttggagatta tgtagatgag
     2821 atgtatgtat caattactaa taatggtcaa tatgttaata ttaaattgaa attatcaaaa
     2881 cgtagtcaaa aatatgagac tgatttcaaa ataaaagtat ggaattttag tgaaagtata
     2941 tagaaataca taaatttaag agaaagaagt gacatcaata agtcatataa aatataatta
     3001 ttgagaacta tataactaaa taatatcaat atgtaaagtg tatagattaa gaatatagag
     3061 gataataata aaaatataga ataagtaatt tgccaaatac ttgtttatat tgtataactt
     3121 atgatattat gatattagta aaaatttgta atcctgactt gacagaattc aagtaagaga
     3181 tataatttta aaggcatagt aatgtaaaat ttactaaaaa ataagaaatt tattttaata
     3241 tggaggaaca aaaaatggta tcagcaggtg attttagaaa aggtgttaca tttgaaaaag
     3301 atggacaacc atgtttagta gttgattttc aacacgttaa gccaggtaaa ggagctgctt
     3361 tcgttagaac taaatacaaa aacttaaaaa caggagctat aagagaagaa agtttcaatc
     3421 caagtgaaaa attccctaaa gctgttatag atacaagaca aatgcaatat ctatacaatg
     3481 atggtgagtt atattatttt atggatcaag aaaattttga acaaatacca ttaaactatg
     3541 agcaagttga agatgcaatt aagttcttaa aagaaaatga agttgctaca ataagatttt
     3601 accaaggaca accattccaa gtggaagcgc caaactttgc agagctagaa gttacagata
     3661 cagagccagg tataaaaggt gatacagcaa gtaatgtaac taaagcagct acagttgaaa
     3721 ctggagcagt tgttcaagtt ccattattta taaatactgg agataaagta aagatagata
     3781 ctagaacagg tgaatattta tcaagagtat aaaaatttat gtatataaaa gcttatatgt
     3841 cttaataata taagatatat gagcttttag tttatatata aaaaagaaag cgaggggtgt
     3901 tacatgaaac atttatatga agaagttgca tacttaaaag gcctagcaga aggactagaa
     3961 ataagtgctg aaagtaaaga agggaaaatg atacataaaa tagttgatgc attagaagta
     4021 tttgcagatg ctattgtaac attggatgaa gaacaagaag aacttcaaga ttttgttgaa
     4081 tctatagatg aagatttagc agatttagaa gaagatattg ctgacatgga agaagatctt
     4141 tatgaagaag atgacgatga ggatgatgag gacttcagct atatagaaat ggaatgccca
     4201 aattgtggag agttagtaga aatagacgaa gatttgttat atgatgatga agtagatgtt
     4261 gtttgtcctg attgtaaggc tgtgatatta tcatcagaag atgattgtga tgatgattgt
     4321 acatgtggtg gttgttcaag ttgtgatgat agagagtaaa aattcataag aattaaaact
     4381 gcctatgatg cctatgagat attttgcttc ataggtagtt ttatttttat gaggaagggt
     4441 attatgtatt ttggttttgc aatacttgga caaaaatagt acactagaat ttatagaata
     4501 ggattttgga gggatgtaaa tatgaaaata agtaaaaaat tactagataa tatacaaaga
     4561 tttgaaaatg ttataaatta taaatttaaa aataaagaat atatactaga agctcttact
     4621 catagttcat attctaatga aaataaaaaa tataatttta acgaaagatt ggaattttta
     4681 ggggattcgg ttttaggcat agtgataagt gattatttat ttaatgaaga agctaatctg
     4741 cccgaagggg aactaactaa acttagagca aatatagttt gtgaagattc attaagtgaa
     4801 gtagctaatg atataaattt aggtattcac atgttattag gtagaggaga agaagcaaca
     4861 ggaggaagac ataggacttc aatattagca gacgcatttg aagctgtaat agctgctata
     4921 tatcttgatg gaggttttga aagtgcaaga cagtttatac ttcatcatat ggaaaatata
     4981 atttatgatt caagaaaagg aaatatattt agagactaca aaacgcatct tcaagaggtg
     5041 ttacaaggga atggagaaaa taatatctgg tacaggctta tagaagaaaa aggaccagac
     5101 cataataaaa gatttgtaat ggaagttgga attaatgacg atgtacttgg tataggagaa
     5161 ggcaagagta aaaaagaagc tgaacagtta gctgctaaaa tagctttaaa gaaaaaatta
     5221 tgggaaaaat aaatataata tgtaaaaata taaaaagaaa tatttatatt agaaggatgt
     5281 tgtagatgaa aaagagaatt atacctatat ttgttcctca caaaggatgt cctcatgatt
     5341 gcatattttg taatcaaaaa aaaataacag gtgtgagtac tgatgttact agtgaagatg
     5401 caagaaatat aattgaggaa tgtttagaga ctatagataa agatgcagat gtagaaatag
     5461 cattttttgg tggtagcttt actgctatag atattgatat acaaaaaagt ttattatcag
     5521 ttgctaaaga atatgtagaa aaaggtcttg taaaagatat tagaatgtct acaagaccag
     5581 attgtattga tgaagatata ttaagtatgt taaaagaata taagacaagt ataattgaac
     5641 taggagtaca atccttagat gaaaaagtat tattagatag tataagaggt catcaatctg
     5701 aaattgtata caagagttca aagatgataa aaaatagtgg aatcaaactt ggattacaga
     5761 tgatgattgg tcttccagct gacacagaag aaaagtgtat atttacagca aaaaaatttg
     5821 tagagctaaa accagattgt gttagagtct atccaacttt ggtagtaaaa gatacaggtc
     5881 ttgaaaagtt aatggaacaa aataaatata atccatttac tcttgaagag agtgtaaata
     5941 tagtaaaaaa agttcttgta ctgttttatg ttaataatat caatgtaata agagttgggc
     6001 ttcaagctac tgatgatata caaattggaa aagctgtttt agcaggacca tatcatcctg
     6061 catttagaga gttggtagaa gcagatatga taaaagatta tttagaattt gttattttgc
     6121 aaaataaaaa tataaagcaa atgttggtca aatctaataa aaagaatatt tctaaaatca
     6181 taggaaataa aaaaacaaat gttaaatata tggaagaaaa atttggtgtg cttttaaaaa
     6241 cacaagaatc agatttagat ataaatcaat tagaaattgt gttagatggg aaaagtttaa
     6301 ttagtgctaa tatgagagac attcatagaa aattgtatga catttatgac ctttagtttt
     6361 aataatgaag ggagagaaaa gatttgtatt taaaaagact agagttaaag ggatttaaat
     6421 cctttccagt aaaaacagat attattttta aagaaggaat aacagccata gtaggaccaa
     6481 atgggagtgg aaaaagtaat atatctgatg ctgtaagatg ggtattagga gaacagagta
     6541 ttaagagtct tagaggtgat aaactcgaag atgtaatatt tgcaggtaca gatacaaaaa
     6601 aacctatgaa ttattgtgaa gtggctctta ctattgataa ttctgaaaat caattggaat
     6661 tagattttac agaagtaact ataagaagaa gggcttatag aaatggtgaa agtgaatttt
     6721 ttctaaataa taaaagttgt agattaaaag atataaaaga agtcttttta gatactggta
     6781 ttggaaaaga tggttattca atcatagaac aaggaaaagt agatgaaata ttaagtaata
     6841 atcctttaag cagaagaaaa gtttttgatg aagcttgtgg catatcaaaa tatagatata
     6901 aaaaacagga agcagaaagg aatttaagta atacaaagga aaatttagaa agaatagatg
     6961 atgtatatat agaaattgaa aatcaattaa agcctctttt taatcaacag acaaaggcaa
     7021 aaaagtactt ggaaataagt gaaaaattaa agacacttga agtaaatagc tttataagag
     7081 aaattgaagg aatagaaaaa gaattaagtg aggttaatga acatcgtaag gttattgaaa
     7141 aggagctcaa tgaaaaagaa gaacaaaaaa atgttgtaga gaaaaagcaa gaagatatta
     7201 ataaagaggt agaggtatta caagatgtaa ttgaaaaatc tgtagattat ataaattcaa
     7261 ttaaaggagt tatatcaaaa aaagagtccc aaataaattt aattaaggaa agaataagaa
     7321 attttacgaa tgaaattagt agaaaaaatt tagaaataaa ggatataaaa gagaagctaa
     7381 atgaaaataa gcaatacata aaagaattag aaagcaataa attatcaggt agtgaagaac
     7441 tatctacctt gcaggaaaat ataaaagtat tggaaggaag taaagataaa caaaaaatta
     7501 agttagaatc cctaaataat gagatagaat tattgaaaga aagtataatt gatatattaa
     7561 ataagaaaca agagtttagt aataagctgt ctactttaaa cgcaaataaa gaaaatatga
     7621 atatcagaga tgaaaatata aattcagaga taacagagtt aaacaaaaat attgaaataa
     7681 aatcatctga attagatact ataaataaag aatttaatat gcaaaatgaa aatttaaaga
     7741 atgtaaataa tagacataaa gaattatcaa ttaatttgca ggattcaatt agtgaacaca
     7801 ataaactaga agatgaaata cagaaaagta aatataattt aaatggatat aattcaaagt
     7861 taaatgtcta tatagatatg gaaaatcatt atgaaggatt taatagagga gtaaaagaag
     7921 tactaaaaaa taaaaattta aaaggagttc atggagcatt aggacagatt ataaatgtac
     7981 ctgaaaagta tgagaaatct atagaagcag ctcttggtgc ttatatgcaa aatataataa
     8041 ctgataatga gtttagtgct aagtttgcaa taaactatct aaaacagaat aatttaggta
     8101 gagtaacatt tcttccatta aatataatta aatcaaataa aataagtcta ggaaatttaa
     8161 aagcaaatac taagttcata ggaatagcaa gtgatttaat tacatttgat gaaaagtata
     8221 gaaatataat tgaaaatata cttggaagaa caatacttat aaataatata gatgagggca
     8281 taaaatttgc aaaagaaact ggtcataggt ttaaaatagt aactttagat ggtgaaatct
     8341 taaatccagg aggttctttg actgggggaa gtctaaaaac taatgggaat atactatcga
     8401 gaaaaagata cattaatgaa tatacagaaa agataagtaa tatcaagaat gaaatttcac
     8461 atctagaatt aaaaagagaa tctttagaca aagatgtgaa aaatatcaaa aatgaaattg
     8521 attcacatga aagtaaaata aaagatttag aaaaaagtat aataataaaa agtacaagta
     8581 taaagaatgt tgagtctgaa attgaatcgt taaaaggtag tattactaaa ttagaaaatg
     8641 agaagaatga tttaaattct aacctaaatt atacacttga aaaaagtgat gatgttagaa
     8701 aagatatgga agagttagat gatttataca ataaaaataa agaaaaaatt gatgcattga
     8761 atgaggaaat aaaaagatac aatgatttat atgataaaga aaaatctgaa tttgatgaat
     8821 tgaacttaag tttagtgaag aaaacagaag tttacaatag tatagttaga gatataaaaa
     8881 gaatatctgg tgaaaattgt gagctagaag aaaaaaataa acaattagaa gagtcattaa
     8941 attatgaaga acatgaaata attaaattac aagattctat actcactgaa gaaaaagaaa
     9001 aagaaaattt aacaaaacaa ctaggggata gtaatagaaa tcttgaaact agaaaaatag
     9061 caaaagatga tttaaaaaat agttttgatg aaattaataa agaattaaaa actatagata
     9121 gacagcatat agaacttaaa gaaagtttat ttaaagttgg tggaagatta gaaagattaa
     9181 aaactagtca agacacatat ataaataagt tgtttgagca atatgatatg accttagtac
     9241 aagcattgga aattaaagat gaagatttgg atatagatag aaaattctta gaaagtctaa
     9301 agagagaaat aagaagtctt ggaaatataa atatagactc tataaaggaa tatgaggaaa
     9361 taaaagaaag atacgatttt tatagcgaac aaaagcaaga tttagaagag tctatggaag
     9421 agatagaaaa acttatacat actttagaag aaaatatgaa atctgaattt gaaattaagt
     9481 ttgaagaaat aagtaaaaat tttaaatatg tatataagag attatttggt ggaggttgtg
     9541 gagaattaac tattttagat aaagaaaatt tattagaaag tgatatacta ataacagctc
     9601 aacccccagg taaaaaaatg aaaaatttaa acctattatc tggtggagag aaagctttaa
     9661 cggcaataag tatattattt gctatattga ttacaaagcc aacaccattt tgtatattag
     9721 atgagattga agcgccactt gatgacgcaa atatatttag atttggagaa tttttgaagg
     9781 atttatctaa agagactcaa tttatatcag taactcatag gagaggaaca atggaagctg
     9841 ctgattatat ctacggagtt acaatgcaag aaaaagccat ttcaaaagtt attagtttaa
     9901 aattaaaaga agctcaggaa ataacagata ttatataact tagattgaca taggaggaat
     9961 aacagtgtta aaaaaattat ttggttttgg aaaagacaag gaaaaggaaa tagaaaaaaa
    10021 agatgctgaa gaagaaatag aagttgaaga cagtgttgat aatttagaaa acttggaaga
    10081 aactattttt tcaggacttg aagaagaagt gatagataaa gtggaagata ttgaagaaaa
    10141 aaatgaggaa agtgtcaata atgaaggcac tgaagaaatc gaaaactttg aaaaaataga
    10201 agttgagatg gatagtaatg gaaaagaagt agaaaatatt tcaaataatg attctcaaga
    10261 agagataatc gaagaattag aaaattatga tgaaattgag tctaaagaga tagaagataa
    10321 agaagataag aaagttaact tgtttgaaag actaaaacaa ggattgacaa aagcaaagca
    10381 aggaataact gatagaatag atgaagtttt aaaatcatat acaaaaatag atgaagaatt
    10441 acttgaagat ttagaggaaa ttttaataac agcagatgtt ggtgtaaata caactatgga
    10501 tataattgaa agactaagag ataaaataaa gcaaaaaggt attactgaac caataaaggt
    10561 aagagaagaa cttaaatcta tagttgaaga tattcttaca aatgaaaact ctacactaga
    10621 tatagaacca gcaccatgta taatattaat ggtgggtgta aatggggtcg gtaagactac
    10681 tacaataggt aaactagcaa atagatacaa aaaagatggt aaaaaagttt tattagcagc
    10741 agcagataca tttagagcag cagcgacaga gcaattagaa atttgggcaa atagaaccaa
    10801 tgtagacata ataaaacatc aagagggagc agaccctgga gctgtagtat ttgatgctat
    10861 aaaagctgca aaagctagaa aaacagatgt attaatatgt gatacagcag gaagattaca
    10921 caataaggct aatcttatga atgaacttgg aaaagtattt aaaatagtag atagagaatt
    10981 tccagaagca aaacgtgaag tacttcttgt ggtggatgct acaacaggtc aaaatgcagt
    11041 tgttcaagca aagacattta aagaagttgc agatataaca ggtatagtac ttacaaaact
    11101 tgatggaact gcaaaaggtg gagtagtcct tgcagtaaaa tcagaagtag atgtacctgt
    11161 aaaacttatt ggagttggag aaagtgtaga agacctacaa gatttcaatg caaaatcatt
    11221 ttcagatgca ttatttggta actaatgttc ttttaaaaat aatggttgac aagagtgttg
    11281 ttatgatata aaatacaata gtgtaaagta aataaccttt acagtatact gctaggaagt
    11341 gtaagatatg aatatagaaa aaatggtcga aattggattg ttatttgaac agtataaaga
    11401 attattaact gacaagcaga aagaaatagt tgctctatac tatgaagaag attattcttt
    11461 aggtgaaatt agtgaaaatc tgaatgtatc aagacaaggt gtttatgata ccttaaagcg
    11521 ttcagaaaaa atactaagag attatgaaga aaagttacac ttagtatcaa agattcaaga
    11581 gcaggaaaaa aatattaaga ttataaaaga taaaattatt gacattaaag aagatttatt
    11641 gcacaataga gattgtgcta atttaatccc taagctagaa aatatagaag atgtatgtag
    11701 ggagatgata aaatgatatt tgaaggattg tctgataaac tacaaggggc atttagtaag
    11761 ttaaaatcta aaggaaaact tacagaagta gatgtaaaaa atgctatgag agaagttaaa
    11821 cttgctcttc ttgaggcaga tgttaacttt aaagtagtta aagatttcat aaaaaaagtt
    11881 caagagagat gtgttgggca agaagttatg caaagcttaa ctccagctca gcatgtaatc
    11941 aaaattgtaa atgaagagct tacaagttta atgggagatg tacaaagtaa agtaatgatt
    12001 tcctcaaagc ctcctacaat tttaatgatg gttggattgc aaggtgcagg taagactact
    12061 acagcaggaa aacttggagg atattttaaa aagcaaggta aaaaacctct attagtagct
    12121 tgtgatattt atagaccagc agctataaag caacttcaag ttgttggaga aaaattggat
    12181 atacctgtat tcaatatggg agataaagaa aatccaataa atattgcgaa agcaggttta
    12241 agccatgcga ttaaaaatgc taatgattta gttattatag atacagctgg tagacttcat
    12301 atagatgaag tacttatgga tgagttaaag tctattaaat cagaagttaa gccacatgag
    12361 atacttttgg ttgtagattc tatgacaggt caagatgctg taaatgttgc agaaagcttc
    12421 aatgaggcac ttggtgttga tggagtagta ttaactaaat tggatggaga tactagaggt
    12481 ggtgctgcgc tttcaataag agcagttaca caaaagccta taaaatttat ggggatggga
    12541 gaaaagctag atgatataga acctttccac ccagatagaa tggcatcaag aatattaggt
    12601 atgggagatg tcttaagttt aatagaaaaa gcacaagaaa atatagactt agagaaagct
    12661 aaagaattag agcaaaaaat taaaaaacaa gagcttgatt ttgaagattt cttacaacaa
    12721 atggaacaaa tacaaaatat gggacctctt gacaagatac ttggtatgat acctggtatg
    12781 ggaaatgtaa aagaccaact tggagatgta gatttgaatg gcaaagaaat gaagagaaca
    12841 aaagccataa ttcagtcaat gactacatat gagagaagaa acccaggagt attaaatgct
    12901 tcaagaaaga aaagaatagc taaaggcagt ggaactagtg tacaagatgt aaatagactt
    12961 ataaaacaac ttaatgaaat gaaaaaaatg atgaagatgt ttgctggttc acaaaagagt
    13021 atgaagaaaa ggggcggctt aggcggttta ccttttttta aataaaatat aaaaaagtaa
    13081 aaattattcg gaggtgactg taatggcagt taaaataaga ttaaaaagaa tgggagctaa
    13141 caaaaaacct ttctacagaa tagtagtagc tgattcaaga gctccaagag atggaaaatt
    13201 tatagaagaa ataggatact ataatccaat ttctgaacct aaacaagtaa gaataaatga
    13261 tgaaaaagct ataaaatggt tagctactgg tgctcagcca acagaagtag ttaaaaaatt
    13321 acttgtaaaa aacggagtaa tagaaaaatt cgaagcttct aaacaagcaa aataattttt
    13381 tcttggcaat tagtttaagt aagggctcat tgctaaggtt actttaggag atgaaatata
    13441 tgaaagagct agttgtagat atagctaagg ctctagttga taatcctgac tcagttgttg
    13501 ttgaggaatt tgaagataat gatggtatcg tcttaaagct tacagttgct caagaggata
    13561 tgggtaaggt tattgggaag cagggaagaa tagctaaagc tataagaact gtcgtaagat
    13621 ctgttgcaaa tagagaaaat ataaaagttt ctcttgagat agtataaaat aggaggatta
    13681 ggtataatgc ctagtccttt ttatataaat gcgtagttta agattttatg atgatatgac
    13741 cacataagat taaatttttt aagaaggtga taacatgaaa gaaaaattaa ctcattttaa
    13801 ggtaggtcaa atagtaaata cacaaggttt aaaaggtgaa gtaagagttt atccgcttac
    13861 tgatgatata gagaggtttg atgaattaaa agatttctat ctgggaaaag atttaaataa
    13921 taaattggct gttgaaaaag taagatacaa aggttctatg gttataatga agataaaagg
    13981 catagacagc atagagaaag cagaaaaact aaaaaataaa tttatgtatg tagctagaga
    14041 agatagcaga gagcttgaag aaggcgaatt ttttatagca gatatgattg gaatggaagt
    14101 tttaactgta gatggaaaat atgtagggat actagaagat gttcttcaat actcagcaaa
    14161 tgatgtctat gtagtaaaag gtgaggaaga taaagaattt atgataccag ctataaaaaa
    14221 atttgtacct acaatagata ttgatgaaag aaaaatgatt atagacccta ttaagggaat
    14281 gatagattag gtggtaatat gagatttcat ataatgacac tttttccaga aatatttaat
    14341 tcgtacatgg atgaaagtat aatgaagaga gcagttgaaa aaggtataat tgaggttcat
    14401 atatataata taagggattt ttctaataat aaacataaaa aagtagatga ttatccattt
    14461 ggaggaggag cggggatggt aatgactcct caacctatat atgacacata taagcatata
    14521 ataacaactc ataatataaa taacccaagt gtaatttatc ttactccaaa aggtaaggta
    14581 tacaatcaat ctatggcaaa gcagatgtct ttaaaagaag atataatact actatgtggt
    14641 cattatgaag gaatagatga gagaatcatt gatttaatag ttactgatga aatttcaatt
    14701 ggagattatg tattaactgg gggagagctc ccagcactta taatgataga ttctatatcg
    14761 agattaatac caggtgtatt aaaccaagaa gaatcatttg aagaagaatc atttaaagac
    14821 aacttattgg aatatcctca ttacactaga cctagagatt ttgaaggatt aaaagtacca
    14881 gaagtgcttt tatcagggaa tcataagaag atagatgagt ggagaagaga agaatctata
    14941 aggataacca aagaaagacg atttgattta tataaaaaat ctaatgaaaa ataaaaatat
    15001 aatgaaaaat taatatatat gtatacaata aagtacctat aaatggaatt aaaaacataa
    15061 aagtgcttgt aataactatt gaagtatagt ataatataaa atgttgaaaa tacgggcgtt
    15121 cctctgttta cactggtcgg gtagttaaga acgtctatga tatctaggag gaagaaagat
    15181 gaatgaaatg ctaagagcaa ttgagcaaga acaattaaaa aatgaagttc ctaattttgg
    15241 acctggggac acagttaaag tacacgttag aattatagaa ggaaaaagag aaagaataca
    15301 agtattcgaa ggtgtagtat taaagagaca aggtggagga gcaagagaaa ctttcacagt
    15361 tagaaaaatg tctttcaatg ttggtgtaga aagaactttc ccagttcact ctccaaaaat
    15421 agaaaaaata gaagttacta gaaaaggtaa agtaagaaga gctaaactaa actacttaag
    15481 aggtagagta ggtaaagctg ctaagataaa agaagctaga aataaataat taaaaacttt
    15541 acttataaat ggggggctga tttatcagtc ctctttctat tttaatgaaa attacagcaa
    15601 ataattatac tagaaataaa tagaaatatt tgttgaaaat atacattatt tatctatgaa
    15661 tttatagctt ttaaaatgaa aaataaaatt ggtaaatatt gcattatatt ttgtaacaat
    15721 attaaaaata agagaacagt attatgtaaa gtatgacgtt aaaaagatga caatattata
    15781 gtttgttaag atagattttt taagtggaat taggatataa taatataata ttaataagga
    15841 gtgatacatg tgtcaaatga atacgaagag tatcttatga atgacaatct acatataaac
    15901 tggtatccag gtcatatgaa aaaaactaag gagttagtaa aaaataactt aaaattgatt
    15961 gatgtagtaa ttgaactttt ggatgctaga atcccgttta gtagtaaaaa tccagacata
    16021 gacaggcttg taggagataa gccaagagtt gtcgtactta ataagagtga tatggcagat
    16081 agggataaat tgaatcaatg gattgaatac tataagaaaa ttaatatcaa agctatacca
    16141 gtagatacaa taaaaggcgt tggtataaat aagataatcg aagaatgtaa aaatgtaact
    16201 agagaaaaaa tgagttcttt aaaagacaaa gggagaaaag aaagagctat acgaataatg
    16261 atagttggag ttccaaacgt tggtaaatcg tctcttataa acaaattaac aggtagaaaa
    16321 agcactcaaa ctggagataa gccaggagta acgaaaggga aacaatgggt tagattaaag
    16381 ggaaatcttg aacttttaga tacacctgga atactttggc ctaagtttga agaccaagaa
    16441 gttgcattaa atttagcatt tagtagagct ataaaggatg agattttaga tactgaaact
    16501 ttggctttaa gacttataga gaaacttatg aaaatagaac ctgagaagtt gaaagccaga
    16561 tataagttag attgtctagg agaaactcca attgaaacta tggatatgat tggccataaa
    16621 agagggttta taacaggtaa aaaagaacta gattatacac gcatagccac aactgtatta
    16681 aatgaattta gagatggaaa aataggaaat ataacattgg aagttccaga aaacgttaaa
    16741 agatagattt taaaacaaaa tcaaaaattt aatgttaaaa atttaaaaaa gccttttagc
    16801 aaaggctttt ttattttttt gaaatataga caaatatctc catataaaag tataaattag
    16861 gttagaaaac ctttaaaaat attaatttaa gacaaatata cctaatattt tgaaaattaa
    16921 aatgagtgtg attatttaaa attttagcat attgacttgc ttgtttcgta taatatataa
    16981 taagcacaaa taaatacaat atattaaaaa tttaatttat ttaattttat taatttaata
    17041 ataagttctt attgtttaat tcaacttact tatgcaagcg taaacaatcg agacatttta
    17101 ttaatttaat aaggttaaaa agggggattt tataatgggt aagaccaagg atgttatagt
    17161 atttggattt gcgttatttg caatgttctt tggagcaggt aacttaatat ttccacctta
    17221 cttgggaata attacaggtc cagaatggtt aattgccttc ttaggattta cgtttgctga
    17281 tgctggattg gcacttctag cagtaatggc tacagctaaa ttcgatggaa acgtagttga
    17341 aatgtttaaa agatgtggta tgaagttagg aattcttata ggttgtgcag atattttatg
    17401 tataggacca ttcttagcta taccaagaac aggtgcaaca acttatgaaa tgggtataat
    17461 gcctttgttt ggaacttcaa ttccagtgtt attattctgt atactttttt ttgcgatttc
    17521 atatgtacta acaataagac cttctaaggt cgtagatata gttggacagt ttttaactcc
    17581 agctcttcta atagctttag catttataat tataaaaggt attatatcac ctcttggaga
    17641 tatagtagat aaacctatga tacctaatgt atttgcagaa ggtataggtc aaggatatca
    17701 gacaatggat gcttttgcag caatagcact tgcatcagtg ctaatagtat ctttaaatga
    17761 taaaggatat tctactatta gtgacaagtt gaaaatgatt ggtaaggcgg gtgttcttgc
    17821 ttgtggagga ttagctctag tttatggagg attatgtttc cttggggcaa ctgtatcaac
    17881 aatgtatgga acagatgcag tacaatcaca agtaatagta aatataactg aaggattgct
    17941 tggaaatgtt gggaaagcga ttttagcagt agtagtatca ttagcatgtt taactacatc
    18001 tataggtctt acatctgcca caggtcaata tttctctaga cttacaaaag gtaaattgag
    18061 ttatgaaaaa atagtattag ctgtatctgt attcagtgca gtagtagcat cttttggtgt
    18121 tggaactata ataaagatag catctcctat actaagtata gtttatccac catcaattgt
    18181 tttaataatt ttagctttct tcaatgaaaa aattaagaat gataatgtat acaaaggagc
    18241 agtatatatg tcattgtttg taagtatact aacagttata tctagttatg gagtagctgt
    18301 tccagtagta aattctttac ctcttaattc attaggattt aactgggtgg tgccagttat
    18361 aatagctgga ataattggta attttatacc atcaaagagt caatctaata cacttggaac
    18421 aaactaagtt ttgtttaatt aaaatataat caattgtaaa taaatgagtt aactaaaagt
    18481 aaaag
//
LOCUS       DAYAPA010000079        18465 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.079, whole genome shotgun sequence.
ACCESSION   DAYAPA010000079 DAYAPA010000000
VERSION     DAYAPA010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18465
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.079"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            96..653
                     /locus_tag="ACGI59_002502"
     CDS             96..653
                     /locus_tag="ACGI59_002502"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFG9740285.1"
                     /translation="MSDSEYPQMKTLETRSCILRPASLDDVEDFFDCYKNKIVVKHLP
                     FSEHKSLEDTRKFIKSFFLNPYKKGKIGHFAIVYKRDNKVIGNMGFNNINPKALEAEI
                     GICINPSYWGHDFATEITKRVIQYGFRELNLNKIIAITYEENQNSTKSLDLLGFKCTG
                     KYIKKIHTGNTVKNVPCYQYELKKQ"
     gene            complement(892..1752)
                     /locus_tag="ACGI59_002503"
     CDS             complement(892..1752)
                     /locus_tag="ACGI59_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFG9740286.1"
                     /translation="MKKQKSPILLEMLGLFFGCISMSIGINMFLKPHTIAPGGLSGLS
                     LVLNKVTGLPVSAIMLIIGVPLVILAFRIMGTKNSLKTLFGTVVFSAIVQLTDPLSKL
                     RFTNDLLLSSIAGGILVGIGLGIMFKSDASTGGTDLIALILSKKFPGIKATKFMSCLD
                     GMVVISSGIVNRSLETALYSGIALCVLIKIADMIMEGFDHSKAFFIISDEPESLRQAI
                     TEELDRGLTILDGKGGYTRENKEVLLVVVSKKQELYLKRLVKRTDPTAFVIVTDVHEV
                     LGEGFKNLEN"
     gene            2251..3654
                     /locus_tag="ACGI59_002504"
     CDS             2251..3654
                     /locus_tag="ACGI59_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFG9740287.1"
                     /translation="MHKPSYKTVFHSYIIFFMIFICLILTVIGIFLSLITIQKPNGEF
                     IRSDWPKKFTENFNEQIVFIDNKPQVKQSGLEKLRENQLWLQILDSSGNRVYGFLEPK
                     EHKDYYSNSELLALANQNNSSNGTVYFNTIKNNKNDYIYIIYFPLNISKVNMFFDGGK
                     FIGGKSIVFSIIGIIFLIILVSGIAYGYWVTKCISNITSAVSDISKRSYLPVNIKGVF
                     ADIYNSLNILNEEIHKSDEIQNKTDIMREEWISNITHDLKTPLSPIKGYAELLTDSST
                     NYSPEKVYKYASIMLKNIEYTNTLINDLKLTYQLKNGIIPLNKNNYDLVRFLKELSID
                     ILNYPEYESNIVEFKSNIDNINFDFDCTLLKRAFSNLIVNAFTYGDKNTKVCVHVTLN
                     ERIEIIISDNGKGMSREEASNLFNRYYRGSNTKQKIEGTGLGLAITKQIIEVHGGNIS
                     VESIPGLGSSFYITFPI"
     gene            3873..6350
                     /locus_tag="ACGI59_002505"
     CDS             3873..6350
                     /locus_tag="ACGI59_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFG9740288.1"
                     /translation="MKIILKYIFTNIKERKIRTAVMLLSIVLSTVLLFVSFSIGLSYE
                     SAQRKMAKGMYGTATISVQSKNPDILTNLEDIPDLNTIKSKVGVLESSAIYNKGGYYE
                     EFSLISADLSQLNKINKPRLENGDSITDFSGDKIILPNRFTSKYKIKKGDSITLQIYG
                     KSYTFQVSDIASYDTVFLRNTRGVNALLPKETLSKIINKGSGYTRVLIESEEEITENL
                     VNKLSEELSTEKYMVSNTINETKIISDARQKTMPFFLISFFALTLSIFIIYSSYKVIT
                     LERLPFIGTFRSIGANEKTVTRILMLESILYGSIGGLIAIPIGVVVLNLMLHGLGNSL
                     EQGISIPVVISPIGVIISVIVAIIVSSFSAYIPVKKASHLPIKNIVLGTVEEKNVSNR
                     SILFIGSIMFILSILLPRISPENTLYLAGGFSLLGLIVATIVLIPLITDIMSIVFEFV
                     YKNILGNEGKLAARNMKNNKNIIQNITLLFISISAVIAISVVGNFVKTYITDVFRDAE
                     LQGFADGNMNEEFIEDVRHMEGIKKILPLYVMNNEISGNGVTLSRLEGTDNIKLYNSM
                     FGINYTNFEIKKQVIEAFNDKRSVILNEDTLKKVGLSIGDTITLSNDKYDFSYKIVGS
                     FKSRANDVEAVIPSHYAVNDFDKTNYGFLVYTAVNPDAVMIQIRYLFGDTYNWSRTVE
                     EFNNDSLNTISSFLSPMNKMTYFIFLLATVGIINNLLINYIQKRRSIAMYKSIGLSNK
                     QNIKVTLIEGVTSGLLGAVIGIVISILEIQTIFIVAGPKISMKPDLDFKTFIIVGLLG
                     IIVTLIGSIVPIIKGKKMKLIEEIKFE"
     gene            6365..7051
                     /locus_tag="ACGI59_002506"
     CDS             6365..7051
                     /locus_tag="ACGI59_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9740289.1"
                     /translation="MKITHNSLAVEAKNIIKEYKIGNTTTRVLKEVSLQVMKGEFVSI
                     MGQSGSGKSTLLYILGGLDTPTSGKVYMNGADISHFNDEKMSIIRRRNIGFVFQFYNL
                     IPNLNVEENIMLPLLLDGKNLKDYKNQLDEILDIVGLTDRRKHTPRELSGGQQQRVAI
                     ARALIGKPEILFADEPTGNLDSKTGIEIIDLLNKINRDNGQTIIMVTHSPEAAKSSSR
                     TITVSDGLIV"
     gene            7325..8158
                     /locus_tag="ACGI59_002507"
     CDS             7325..8158
                     /locus_tag="ACGI59_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFG9740290.1"
                     /translation="MLKKESIHFINKNRSLIVCEDNFVYLLPCIQLRNWISNFTISFP
                     NQTIISDNYTIIPHGSVTLVFFYDATGLHSLLFGPTTKPKTVGNIANRCDVILIIEFQ
                     PAGFFPLIGIQQSELIDKVVPFSIINTSLDLEIKKIFNESLSIDKLILKLEELLISNI
                     KIEYSYEFILAIQLIIQNSGNISSQEISKKVFYSSRHLNRLFNQYLGLSMKSFSRLVR
                     INKSIKLLNNNKTSWMSICNELGFYDIPHFIKDFKIVCGITPQKYRANMSDFYSEIAK
                     F"
     gene            8196..8657
                     /locus_tag="ACGI59_002508"
     CDS             8196..8657
                     /locus_tag="ACGI59_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFG9740291.1"
                     /translation="MKFGGVLLIVKDINKSKKFFEDVMEQKITLDLGEHVSFENGFGL
                     QSNYKAIIGYDLPIYQQSNNFQLYFEVNDIKQWESKINRIGNIEFLHYIKEYPWGQRT
                     FRFYDFDKNIIEISESMESVIKRLLKQGLALEEISKRTMYPVEFITQFQQV"
     gene            8688..8936
                     /locus_tag="ACGI59_002509"
     CDS             8688..8936
                     /locus_tag="ACGI59_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFG9740292.1"
                     /translation="MQKTKAWGIIEPEKPESQQQMAHMDFAVKDLEEAVQYAIHCGAT
                     IAEEQFTDDWRVMIDPAGHPFCLCQMKSIMESSHFSLL"
     gene            9389..9709
                     /locus_tag="ACGI59_002510"
     CDS             9389..9709
                     /locus_tag="ACGI59_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein HesB"
                     /protein_id="HFG9740293.1"
                     /translation="MKITLPETAIDTLKSILKDNQDKPNNIRVYFAGVGCGGPSFGLA
                     LDEKKEDDLTYEVGELQFVMSSDEYSQYGDIIIEDTGFGFRVIPENMKDQGGGGCSGC
                     SGCH"
     gene            9847..10491
                     /gene="trmB"
                     /locus_tag="ACGI59_002511"
     CDS             9847..10491
                     /gene="trmB"
                     /locus_tag="ACGI59_002511"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFG9740294.1"
                     /translation="MRRRRKKGADEKLLSYTKYVLRDDIDKLKGKWNLKFRNDNPIHV
                     EFGTGKGKFITTLAKQNPDINYIAMELKEEVLLKAVEKADASNLNNILFLWGDVSNIL
                     DYFEAKELSRIYINFCDPWPKNRWSKRRLTHSGFLEMYNRVLEDDGEIHFKTDNEKLF
                     EFSLNEIAANNWLLKNISLDLGNSEYENNVTTEYEDKFMSQGMRIFRCEAKKRN"
     gene            10635..11366
                     /locus_tag="ACGI59_002512"
     CDS             10635..11366
                     /locus_tag="ACGI59_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9740295.1"
                     /translation="MNLANLIPFIISAAALSPINSNDKVLSENQLKDIFPDANLRAVV
                     KRYINPDEMTISNIKALDGEFYATGESISNLKGISYLENVDNFIFWNNNIKEVPKEAL
                     SLKDMDSINLANNYLIDDDVVNSLSHNGVDVNCDLNFIDTKDNQYKLDSKYHNVDIAK
                     GESIDLRKIITKKIDSYYKYWEVTDNLPKDLNFIVSVSDKSVLSCEDMIIKGNKTGKA
                     VVKVILSDKNDLNTSQEVLITVNVK"
     gene            complement(11495..11773)
                     /locus_tag="ACGI59_002513"
     CDS             complement(11495..11773)
                     /locus_tag="ACGI59_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9740296.1"
                     /translation="MVDTLEFGLKILFFILSIIWMGKIMILRTDKQIVINPLLIGISA
                     ILVMLHTSQSNIEFFGLDVQYIRIVLYIIYSLIILIGIWATNRRNGIF"
     gene            12048..12494
                     /locus_tag="ACGI59_002514"
     CDS             12048..12494
                     /locus_tag="ACGI59_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9740297.1"
                     /translation="MESKFFNWYTQSLGGTFGIISCICAYLNGYMFVYTNSASKYFES
                     IGFLGIISSYTLLPLCMLTLIFGVIYSYTPETSDKSIFNIPFFTLNKRLILITVVFGF
                     IGARVYFIIPAILITFNLYSNVLIAKLKYILCKNKENDTNLKESEV"
     gene            12764..13216
                     /gene="accB"
                     /locus_tag="ACGI59_002515"
     CDS             12764..13216
                     /gene="accB"
                     /locus_tag="ACGI59_002515"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFG9740298.1"
                     /translation="MNINEIKELLKAIDSTNLEYVKLESSDLRLEVSKKAQSTSSPVL
                     SVQQESVVDLSLEKPVVNDTPVTSNENLSVVVAPLMGTFYDSPSPDADSFVKVGDVVE
                     EGDTLCILEAMKLMNEITSEIKGEIIEVLVSNEELVEYNQPLFKIKPL"
     gene            13232..14593
                     /gene="accC"
                     /locus_tag="ACGI59_002516"
     CDS             13232..14593
                     /gene="accC"
                     /locus_tag="ACGI59_002516"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFG9740299.1"
                     /translation="MIKKILVANRGDIAVRIIRTCKELGIKTVAIYSEIDKDCFHRYI
                     ADESICIGPNNISKSYNNIENIIYLALKLKCDAIHPGFGFLSENPEFAKQCEDNNIIF
                     IGPTREQMILMGDKSRARETMMELNIPVVPGSESVLKTKEEALEVAREIGYPVMIKAS
                     SGGGGKGMRIVRKEEELFSNFDMASSEALAAFSNSDLYMEKFIENPRHIEVQVFGDKH
                     SNAIHLGDRDCSMQRRNQKVIEESLSPYLSDEERQKLHKIAVDIVKGVGYIGAGTIEF
                     IVDKDKNFYFIEMNTRIQVEHPVTEMVTNLDLIKLQISIANGDKIPFKQEDITFRGHA
                     IECRINAEDSSKNFAPSPGKIESLNLPGGFGVRFDTFVYAGYTIPPLYDSMIGKLICW
                     AETREECISRIYRALDEIIVEGINTNVEFQKALVTSEEFREDTHHTKFIEDVFMKKEF
                     ATL"
     gene            14608..15462
                     /gene="accD"
                     /locus_tag="ACGI59_002517"
     CDS             14608..15462
                     /gene="accD"
                     /locus_tag="ACGI59_002517"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFG9740300.1"
                     /translation="MIKKFLSSKESKYVTISLDDNFKKNSVDDKFWTYCKGCDSHVFR
                     KDIEENSFVCPKCSRHYGLRARKRINLLIDKGTFMEFNSDVEFQNPLNFPKYKEKVDS
                     YKEKTKESEAVVTGYGRINGIKTVICVMNPDFMMGSMGSIVGEKITYSIEYAAENNLP
                     IIICSASGGARMQEGMVSLMQMAKTSQALSKLEEKSLPYISVLTDPTTGGVTASFAML
                     GDIIISEPNTLIGFAGPRVIEQTINQKLPEGFQTSEFLLEKGFIDMIVDRRKMKEVLY
                     QILAMHKK"
     gene            15483..16427
                     /locus_tag="ACGI59_002518"
     CDS             15483..16427
                     /locus_tag="ACGI59_002518"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HFG9740301.1"
                     /translation="MIENNSEKIKVLENDIKQLITISKANNIDLSDKINSLNEKLEKL
                     KEDAFSHLSAYEKVTLSRDIKRPTTLEYIEHICSNFLELHGDRLFKDDPSMVGGIGQI
                     GKFNVTIVGHQKGRDTKENIKRNFGMPHPEGYRKALRLMKQAEKFNRPIITFIDTSGA
                     FCGLEAEERGQGEAIARNLLEMSKLSVPVITFVIGEGGSGGALGIGVGNDVCMLEHSV
                     YSVISPEGLSSILFKDSSKAKEACDVMKLTSNDLYDLKIIDKIIKEPLGGAQKDVEAV
                     SKEIKAYILERLNHYKDMDKEEIIAQRYKKFRNIGKCL"
     gene            16616..16876
                     /locus_tag="ACGI59_002519"
     CDS             16616..16876
                     /locus_tag="ACGI59_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
                     /protein_id="HFG9740302.1"
                     /translation="MEVLKVSSKSNPNSVAGALAGVLRERGVAEIQAIGAGALNQAIK
                     SIAIARGFVAPSGMDLVCIPAFTDIEIEGDKKTAIKLIIEPR"
     gene            17029..17400
                     /locus_tag="ACGI59_002520"
     CDS             17029..17400
                     /locus_tag="ACGI59_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HFG9740303.1"
                     /translation="MKLKLLVEEYAVCRLNNDSKIPTWIDTEKFYSITRTDDELSVVC
                     LNNNIPSDVKSEKEWRILKILGPLDFSLVGILSKLSSLLADNKISIFAISTYDTDYIL
                     VKEKDIENACKILSCNGYEVE"
     gene            17430..18275
                     /locus_tag="ACGI59_002521"
     CDS             17430..18275
                     /locus_tag="ACGI59_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9740304.1"
                     /translation="MIYIIEILKISVINIFCFIGIFIVFGLIFSITENLNNKFIFSSF
                     GKTGIIVTGAIGTFVHELSHLIMCLIFMHKINSVKFFRPIESKNDGILGYVSHSYKKD
                     NLYQSIGNFFIGIAPIIGGTIMIILIFKILLPDSYMKVTQNIDLKLYVSMINNLNING
                     FIHIILGDISNFIRIMLLTPSVYSLKYFIFMFLMYSISTHMSLSSADLKNSLNGLSFI
                     VIVIFVISSITYLFGFDNLNISSLIVKYNIFISFFMAIGFIFSIVTLIISYILSLVSP
                     FKNFS"
BASE COUNT         7137 a         1987 c         2925 g         6416 t
ORIGIN      
        1 taaaaataag gttaatttga gaacatttat ataattaata tcgtatttta aatatgaaca
       61 gtatatttag attaatgtat aaagggggat tagaaatgtc tgattctgaa tatcctcaaa
      121 tgaaaacctt agaaactaga agttgtatac taagacctgc atcattagat gatgttgaag
      181 atttttttga ctgttataaa aataaaatag tagtaaaaca tttaccattt agtgagcata
      241 aatctttaga agatacaaga aagtttataa aatcattttt tctaaatcca tataaaaaag
      301 ggaaaattgg ccactttgct atagtatata aaagagacaa taaagttatt gggaatatgg
      361 ggtttaataa tataaatcct aaagcattag aagcagaaat aggtatatgt ataaatccaa
      421 gctattgggg acatgatttt gctactgaga taacaaaaag agtaatacag tatggattta
      481 gggaactaaa cttaaataaa ataattgcaa taacctatga agaaaaccaa aattctacaa
      541 aatcattaga tttattagga tttaaatgta caggaaagta tatcaaaaaa attcatactg
      601 gaaatactgt aaaaaatgtt ccttgttatc aatatgaatt gaaaaaacaa tagatataat
      661 catttaatat ctatgctggt tatttagtaa tgattgtttt ctaaataaaa tttcaataaa
      721 gtggttgcct caaaatatag ataatcctgt ttttattgtt aaaatactga aaatgcattt
      781 gattttatac aaaaaagagt gtctcatgaa ctaaaaagtt tatgagacac tctttttgaa
      841 taaatcgtat tttaattaaa atagtatata tacttaaaat attaattttt attagttctc
      901 taaattttta aatccttctc caagaacctc atgtacatct gtaacaataa caaatgcagt
      961 agggtcagtt cttttaacta atctctttag ataaagttct tgttttttag aaactacgac
     1021 taatagaact tctttatttt ctctagtgta tccaccttta ccatcaagaa tagtaagacc
     1081 tctatcaagt tcttcagtta tagcttgtct taaactttca ggctcatcag agataataaa
     1141 aaatgctttt gaatggtcaa atccttccat tatcatatct gctattttta ttaatacaca
     1201 aagtgctata ccagagtaaa gagcagtttc taaactacga tttactatac cagaagatat
     1261 aacaaccatt ccatctaggc atgacatgaa ttttgtagct tttattcctg ggaatttctt
     1321 actaagtatg agtgcaatta agtctgttcc tccagtagaa gcatctgatt taaacattat
     1381 tcctaaacct ataccaacta ggattcctcc tgcaatagaa gacaataata agtcattagt
     1441 aaatcttagt tttgataatg ggtctgttaa ttgaactata gctgaaaata caactgttcc
     1501 aaataaagtt tttaatgaat tttttgtacc cataattcta aaagcaagta ttactaatgg
     1561 gacacctata attaacatga tagctgatac tggaagtcct gtaaccttgt ttagtactaa
     1621 tgatagacca ctaagtcctc caggagcaat tgtatgtggt tttaaaaaca tatttatacc
     1681 aatactcatt gatatacaac caaaaaatag tccaagcatt tcaagtaaaa ttggcgactt
     1741 ttgttttttc atataaacac ctccatgaac ttgtcacaat ttaatattat aacatatttg
     1801 accagtttat attatataaa atttcttgat ataagcaatt tcaagtattt cattattctt
     1861 aatatttttg aatttttata tagtgttttc atatctttac tgatgttgtt acactcctat
     1921 gaagtttttt atatattata atttttttat tcttttataa agtatcccct ataaagatgg
     1981 ttctatgaaa attttctcta tattgtttaa cgtatataga gattttgtgt ataattataa
     2041 ggtatttaat ggaaaaattt atcttacttt tatatgttat caaaatgatt tattaaatac
     2101 aacagacagc ttgataacat gtaattatat aaatataatt tatgtagata gttgtatttt
     2161 taaataaatt tttacatatc aataattatt gtctatttta tataagcaac aatcaaacta
     2221 aaataaaagt ttaagaaaag agttgattaa atgcataaac ctagttataa aacagtattt
     2281 cattcataca ttattttttt catgattttt atttgcttaa tcctgacagt aataggtatt
     2341 ttcttatcac ttattactat tcagaaacca aatggggaat ttattcgtag tgactggccg
     2401 aaaaaattta cagaaaactt taatgaacaa atcgtattta tagataataa gccacaagta
     2461 aaacagtctg gattagaaaa acttcgagaa aatcaattgt ggttgcaaat tttagattct
     2521 agtggaaata gagtttatgg gtttttagaa ccaaaagagc ataaagatta ttattctaat
     2581 tctgaattat tagcattagc taaccaaaat aattcaagta atggaactgt atactttaat
     2641 acaattaaaa ataataaaaa tgactatatt tatattatat attttccatt aaatatatct
     2701 aaagtgaata tgttttttga tggaggtaaa tttataggtg gtaagtcaat tgtattttca
     2761 atcataggga ttatattttt aataattttg gtttctggaa ttgcttatgg ttactgggta
     2821 acaaagtgca tatcaaatat tacatctgct gttagtgata tttcaaaacg aagctatctt
     2881 ccagttaata ttaaaggtgt ttttgctgat atatataata gtttaaatat tttaaatgaa
     2941 gaaattcata aaagtgatga gatacaaaat aaaactgata ttatgagaga agagtggatt
     3001 tcaaatataa ctcatgattt aaaaactcca ctttcaccaa taaaagggta tgcagagctt
     3061 cttacagata gtagtactaa ttattcacct gaaaaagtat ataaatatgc ttcaatcatg
     3121 ttaaagaaca tagaatatac aaatacactt ataaacgatt taaaattaac ctatcaatta
     3181 aaaaatggaa taatcccttt aaataaaaat aattatgatt tagtaagatt cttgaaagaa
     3241 ttatccattg atattttaaa ttatccagaa tatgaatcaa atatagttga atttaagagt
     3301 aatatagata atattaactt tgattttgat tgtacactat taaaacgagc ttttagtaat
     3361 cttattgtca atgcttttac atatggagat aaaaatacta aagtttgtgt acatgtaaca
     3421 ttaaatgaaa gaattgaaat cattatatct gataatggaa aagggatgtc aagagaagaa
     3481 gcatcaaatt tgtttaatcg ttattataga ggaagcaata caaaacaaaa aatagaagga
     3541 actggtttag gactagcaat aacgaagcaa attattgaag ttcatggagg gaatatatct
     3601 gttgaaagta tacctggatt aggtagctca ttttatatca cctttcctat ttaattaagg
     3661 ttaattaagg ttaaataaaa aatatattaa ggttgataga ttattatatt tatatagtag
     3721 tttatcaatc ttttttattt aaggggtgat ttgattaaaa atattataaa tagaatgata
     3781 aaagtatgga ggagtctatt agagatttaa tttagattaa agtataaaaa tattagattg
     3841 aaatatctta gataagggaa gaaggtgtaa gtttgaaaat tatattaaag tatattttca
     3901 caaatattaa agaacgaaaa attagaacag cagttatgtt attgtccata gttttatcaa
     3961 cagttttact ttttgtttca ttctcaattg gattatctta tgaaagtgca caaagaaaaa
     4021 tggcaaaagg tatgtatggt acagcgacta tatctgtcca aagtaaaaat ccagatatat
     4081 taaccaattt agaagacata cctgatttaa atactataaa atcaaaggtt ggtgtattgg
     4141 aaagctctgc tatttataat aaagggggat actatgaaga atttagtctt atatcagcag
     4201 acttaagtca actaaataaa atcaacaagc cacgattaga aaatggcgat agtattactg
     4261 atttttctgg tgataaaatt attcttccaa atcgctttac atcaaaatat aaaataaaaa
     4321 aaggtgattc tattactttg cagatttatg gaaagtctta tacttttcaa gttagtgata
     4381 ttgcatctta tgatacagta tttttgagga acacaagagg tgtaaatgca ctgctaccca
     4441 aagagactct atcaaaaatt ataaataaag gcagtggata tacaagagtt cttatagagt
     4501 cagaagaaga aattacagaa aatctagtaa ataaattatc cgaagaactt tctactgaaa
     4561 aatatatggt atcaaatact ataaatgaaa ctaagataat ttcagatgct agacaaaaga
     4621 caatgccttt ctttttaata agtttttttg cattaaccct tagtatattt ataatataca
     4681 gcagttataa ggttattaca ttagaacgtc ttccttttat tggaacattt agaagtattg
     4741 gtgccaatga aaaaactgta actcgtatat tgatgcttga aagtatatta tacggaagta
     4801 taggaggatt aattgctatt cctattggtg tagttgtttt aaatttaatg cttcatgggt
     4861 taggaaattc tctcgaacaa ggaatatcaa ttccagtagt aatatcaccc attggagtga
     4921 ttatatctgt tatagtagca ataatcgttt cttcatttag tgcatatata cctgttaaaa
     4981 aagctagcca tttgccgata aaaaatattg tactaggaac agtagaagaa aagaatgttt
     5041 caaatcgttc tatacttttt ataggaagta taatgtttat tttatctatt ttacttccaa
     5101 gaatatcccc tgaaaataca ttgtatctag ctggtggatt ttcattacta ggattaattg
     5161 tagctactat tgtgttgatt cctcttatta ctgatatcat gtctatagtt tttgagtttg
     5221 tttataaaaa tattttaggg aatgagggta aattagctgc tagaaatatg aaaaataaca
     5281 aaaatataat acagaatatc actttactat ttataagtat ttctgcagtt attgcaataa
     5341 gtgttgttgg taactttgtc aaaacttata taacagatgt gtttcgtgac gcagaattac
     5401 aaggttttgc agatggaaat atgaatgaag agtttattga agatgttaga catatggaag
     5461 gaattaaaaa aatattacct ctttatgtta tgaataatga gatttctggt aatggtgtaa
     5521 cattatcaag actagagggt acagataata taaaactata taactctatg tttggaatca
     5581 attatacaaa ttttgagata aaaaaacaag taatagaagc ttttaatgat aaacgttctg
     5641 ttattttgaa tgaagatact ctaaaaaaag taggcttatc tataggagat acaataactc
     5701 tatctaatga taagtatgat ttttcttata aaatagtagg tagttttaaa tcccgtgcaa
     5761 atgatgtaga agctgttata ccatcacatt atgcagttaa tgattttgat aaaactaatt
     5821 atggtttttt agtatataca gcagtcaatc cagatgctgt tatgattcag ataagatatt
     5881 tatttggaga tacatataat tggagtcgta cagttgaaga atttaataat gattctttaa
     5941 atactataag tagttttttg tctcctatga ataagatgac ctatttcatt tttcttcttg
     6001 ctacagtagg tattattaat aatctactga ttaattatat tcaaaaaaga cgttctatag
     6061 ctatgtataa atctatagga ctaagtaata aacaaaatat aaaagtaaca ttgattgaag
     6121 gggttacttc aggtttactt ggagctgtta ttggtattgt gatatctata cttgagattc
     6181 aaactatctt tatagttgca ggaccaaaaa tttcaatgaa accagattta gattttaaaa
     6241 catttattat tgttgggtta ttgggaatta tagtaacttt aataggttcg atagtgccta
     6301 ttataaaagg gaagaaaatg aaactgatag aagaaattaa gtttgaataa ttatggaggt
     6361 taatatgaaa attacacata atagtttagc tgtagaagca aaaaatatta tcaaagaata
     6421 taaaattgga aatacaacaa ctcgagtgtt aaaagaggta tctttacaag taatgaaagg
     6481 tgaatttgtt tctatcatgg gtcaatctgg ttctggtaaa agtactcttt tatatatttt
     6541 aggtggttta gatactccaa cgagcggaaa agtgtatatg aatggagctg atatatcaca
     6601 ttttaatgat gagaaaatga gcataatacg tcgtagaaat ataggattcg tatttcaatt
     6661 ttataactta ataccaaatt taaatgtgga ggaaaatata atgcttcctt tgttattaga
     6721 tggtaaaaat ttaaaagatt ataaaaatca attagatgaa atcttagata tagttggttt
     6781 aactgataga cgtaaacata caccacgaga gttatctggt ggtcagcaac aacgtgttgc
     6841 tatagctcga gctttaattg gaaaaccaga gattctattt gctgatgagc ctactggaaa
     6901 tttagatagt aaaacaggga tagaaataat agatttactg aataaaataa acagagacaa
     6961 tggacaaact ataattatgg taactcactc accagaagca gccaaaagta gtagtcgtac
     7021 tattacagtt tcagatggtt taattgtata aggactaatt attgtatttg agataagcat
     7081 ttttaattat ataaattaga catatgtaaa gtggatataa gttaaatact ataaatgtgc
     7141 tataatatta aaataacaga taaaagtact atatttttta ttgattattt taattttatt
     7201 tgtttattaa atatctaatg agtattcata ctatctctat tattaaaagc agttaatgat
     7261 atatagagat aatatggatt cttcttactt taataaatat tcacgatagg acaggatata
     7321 atatatgtta aaaaaagaaa gtattcattt tataaataaa aataggtcac ttatagtttg
     7381 tgaagacaat tttgtttatc tcttgccttg tatacaatta agaaattgga tatcgaattt
     7441 tactatatcg tttccaaatc aaacaataat ttctgataat tacacgatta ttcctcatgg
     7501 aagtgtaact ttggtgtttt tttatgatgc cacagggtta catagtctat tatttggtcc
     7561 aactactaaa ccaaaaacag taggaaatat tgctaataga tgtgatgtta tactaattat
     7621 agaatttcaa cctgctggtt ttttcccgct tataggtata cagcaaagtg aacttattga
     7681 taaagttgtt ccattttcta taattaatac ttcattagat ttagaaatta aaaaaatatt
     7741 taatgaatct ttatctatag ataaattgat tttaaagttg gaagaattat tgatttctaa
     7801 tattaaaatt gaatactcat atgagtttat tttggcaatt cagttgatta ttcaaaactc
     7861 tggaaatata tcttcacaag aaatttctaa aaaagtattt tatagttcta ggcatttaaa
     7921 cagactcttt aatcagtatc tagggttgag tatgaagtca ttttctcgtt tggtacgtat
     7981 taataaatct ataaaacttc taaacaataa taaaacttca tggatgtcta tatgtaatga
     8041 attaggattc tatgatatac cacattttat taaagatttt aaaattgtct gtggaataac
     8101 tcctcaaaaa taccgagcta atatgtctga tttttacagt gaaattgcaa aattttaagt
     8161 tataatactt ataacaaaga aaggatagtg ataagatgaa atttggagga gtattgctaa
     8221 ttgtcaaaga cataaataaa tcaaaaaagt tttttgaaga tgttatggag caaaaaatca
     8281 cattagattt aggagaacat gtatcatttg aaaatggatt tggattacaa agtaattata
     8341 aagctataat tggatatgat ttaccaatat atcaacaatc aaataacttt cagttgtatt
     8401 ttgaggttaa tgacatcaaa caatgggaaa gtaagataaa tagaatagga aatattgaat
     8461 ttttgcatta tataaaagaa tacccatggg gtcaaagaac ttttaggttt tatgattttg
     8521 ataaaaatat tatagaaatt tcagaaagta tggaaagcgt cataaagagg cttttaaaac
     8581 aaggtttagc tttagaagaa atttcaaaac gcactatgta tccagttgaa tttattacac
     8641 aatttcaaca agtataaaat attaaaatat ataatttttt ctttatattg caaaaaacaa
     8701 aggcttgggg cataatagaa ccagaaaaac ctgaatctca acagcaaatg gctcatatgg
     8761 atttcgctgt taaggattta gaagaagcag ttcaatatgc aattcattgt ggagctacaa
     8821 tcgcagagga gcaatttact gatgactgga gagttatgat tgacccagct ggacaccctt
     8881 tttgcttatg tcagatgaaa tcaattatgg aaagtagcca tttttcattg ttatagacca
     8941 aaatattgtt tattaattac aaaaaagcac aacagtatat accgttgtgc ttttacttat
     9001 tatacatgaa tacactaaaa aatataaata gtagagcttt atcaataatg ataattttta
     9061 aaaaatagta tttttttatt gaataatttc ttagatattt taaagtatat aaattttgca
     9121 tatatgaatt ggacattatc aaatgtgtta taatattaaa acaacaaata aaattattat
     9181 attttttaga ataataattt tatttgttta ctaagtttat gatagggtat ttatttaagg
     9241 taataaaata ttatataaaa tattttaaag ccttaggtaa tattaggtag aagttttagt
     9301 aaaatatgca aaaactaatt gattatgtac cttgtatggg tatataatag atattgtttt
     9361 aataaaatat ttgaaaggga atgatttgat gaaaattact ttaccagaaa cagctattga
     9421 tactttaaaa agtatcttaa aagacaatca agataaacct aataatataa gagtatactt
     9481 tgcaggtgtt ggttgtggag gtccttcttt tggattagct ttagatgaga aaaaagaaga
     9541 tgacttaact tatgaagttg gagaattaca atttgtaatg agctctgatg aatatagtca
     9601 gtatggagat ataataatag aagatacagg atttggattt agagttattc ctgaaaatat
     9661 gaaagatcaa ggtggaggag gatgctctgg ttgttcaggg tgccactaag attaagtttt
     9721 tagtaagttt agaactctct ttatagaaat aaatttctat tttaagagag ttctttttat
     9781 tttttggtga gataatgtat aataaaaatg tattttacta attttttata tgtaaggaga
     9841 attattgtga gaagaagacg aaaaaaaggt gcagatgaaa agcttttaag ttatacaaaa
     9901 tatgttttaa gagacgatat agacaaatta aaaggcaagt ggaatttaaa gtttagaaat
     9961 gataatccaa tacatgttga atttgggact ggaaaaggta agtttataac aactttagct
    10021 aagcagaacc ctgacataaa ttatattgcc atggaactta aggaagaagt tcttttaaaa
    10081 gctgttgaaa aagcagatgc ttctaatttg aataatatac tatttttatg gggagatgta
    10141 agcaatattt tggattactt tgaagctaaa gaattatcga gaatttatat aaatttctgt
    10201 gacccatggc ctaaaaatag atggagtaaa agaagactca ctcattctgg atttttagaa
    10261 atgtacaata gggttttgga agatgatggc gaaattcact ttaaaacaga taatgaaaaa
    10321 ttattcgagt ttagtttaaa tgaaattgct gcaaataatt ggctattaaa gaatatttct
    10381 cttgatttag gaaatagtga gtatgaaaat aatgttacaa cagagtatga agacaaattt
    10441 atgtcacaag gtatgcgaat atttagatgt gaagctaaaa aacgtaacta gtaatatgtt
    10501 tataagttta atagtagaaa tttattttaa tttatattaa taaagtgtac gaaagtaggt
    10561 atatataaca aggaaacaga accctctcat agaaattaaa ttattataat aaaaaattta
    10621 tgtgggaggg tactatgaat ttagctaatt taataccatt tattatttct gcagcagctt
    10681 taagtccaat aaattcaaat gataaggttt tgagcgaaaa tcaacttaaa gatatttttc
    10741 ctgatgcaaa tttaagagca gttgtaaaaa gatatataaa tccagatgag atgactataa
    10801 gcaatataaa ggctttagat ggagaatttt atgcaactgg tgagagtatt tcaaatttaa
    10861 aaggtatatc atatcttgaa aatgtagaca attttatttt ttggaataat aatataaaag
    10921 aggttcctaa ggaagcttta agtctaaaag atatggattc aataaatctt gccaataatt
    10981 atttgattga tgacgatgta gtaaattctc tttctcataa tggtgtggat gtaaattgcg
    11041 atttaaattt tattgataca aaagataatc agtataaact ggactctaaa tatcataatg
    11101 ttgatatagc aaaaggtgaa agtattgatt tgagaaaaat aattacgaag aaaattgata
    11161 gttattataa atactgggaa gttactgata accttccaaa agatttaaat tttattgtat
    11221 ctgtaagtga taagagtgta ctttcatgtg aagatatgat aattaaaggt aataaaacag
    11281 gtaaagcagt ggtaaaagtt atactaagcg acaaaaatga tttaaataca tctcaagaag
    11341 ttctaattac tgtcaatgta aaataatctg tcaacttatt caatcattct attttcaatt
    11401 taagtgtata atcaaaataa aggttgttag taacagaacg ctaatattag tgatttgtta
    11461 ctagcaacct ttaaatttta tagaaataga ttatctaaaa aattccattt cttctgttag
    11521 tagcccatat tcctattaaa attatcagtg aatatattat atacaaaact attcttatat
    11581 attggacatc taatccaaaa aattcaatgt tactctgtga ggtatgcaac atcacaagga
    11641 ttgcagatat accaataagc aaaggattga ttactatttg cttatcagtt cttaaaatca
    11701 ttattttacc catccaaatt attgataata taaaaaataa aattttaaga ccaaattcta
    11761 atgtatctac caaagcgaaa acatcccttc atttataata aatttttcag ctcaacaaaa
    11821 tataaaaccc tacaaacaac ttttgaatcc ataataagat aacttgatta agcactatga
    11881 aataagtact ataaaacttc atctacatta atatatacta cactatactt caaatttctt
    11941 aaacataaat taatgaaatt aacaaaattt tattcagaaa catataaaga tatattttac
    12001 tatttaaatg catatcatta tatatacagt tttaggaggt gaggtatatg gaatctaaat
    12061 tttttaattg gtacactcaa tccttagggg gaacttttgg cattatatca tgtatatgcg
    12121 catatttaaa tggatacatg tttgtttata caaatagtgc tagcaaatat tttgaaagta
    12181 taggattttt gggaataatt tcaagttata cactacttcc actttgtatg cttactttga
    12241 tttttggagt tatatattct tatactccag aaacatccga caaaagtatt tttaatatac
    12301 ctttttttac tttaaataaa agattaatac ttattacagt agtttttggt tttataggtg
    12361 ctagagttta ctttataatt cctgcaattc taataacttt taacctatat agcaatgttt
    12421 tgatagctaa attaaagtac atcctatgta aaaataaaga aaatgatact aatttgaaag
    12481 aaagcgaagt ctaaattgca taatattggt atttgtctta aaaattcccg aggtattaat
    12541 ttgtattctc ttgtattttg caaaaaatat tgaattttat tcattttata cttataattt
    12601 atattaataa tggaaaatta aaacaaatat aagttactgg ttgttaattt aaaattaaaa
    12661 gatatattga tgtgttgatt ctctagtcag cactttttct tttataaatt tgttttttgt
    12721 gatataatat tagcaccagg taataataca agggggtaac aaaatgaaca tcaatgaaat
    12781 taaggaatta ttaaaggcca tagactcaac taacttagaa tatgtaaaac ttgaaagtag
    12841 cgatttaaga ttagaagtat ctaaaaaagc tcaatctaca agttcacctg ttttaagtgt
    12901 acaacaagaa tcagtagtgg atctttcttt agaaaagcca gttgtaaacg atacacctgt
    12961 cacatctaat gagaatctat cagtagtagt agctcctttg atgggaacat tctatgattc
    13021 tccaagtcca gatgcagata gctttgttaa agttggcgat gtagttgaag aaggcgacac
    13081 tttatgtata ttagaagcaa tgaagcttat gaatgagata acaagtgaaa ttaaaggtga
    13141 aataattgaa gtattagtta gtaatgaaga actagtagaa tataatcaac ctttatttaa
    13201 gataaaacca ttgtagtagg aggtatttaa gttgataaaa aaaatactag ttgcaaatag
    13261 aggcgatata gcagttagaa tcatcagaac atgtaaagaa ctaggtataa aaactgttgc
    13321 tatctattct gaaatagata aagattgttt tcatagatat atagctgatg aatctatttg
    13381 tataggacca aataatataa gtaagagtta taataatata gaaaatataa tctatcttgc
    13441 tcttaaatta aagtgtgatg ctattcatcc aggctttgga tttttatcag agaatcctga
    13501 atttgcaaag caatgtgaag ataataatat tatttttata ggtccaacaa gagaacaaat
    13561 gattttgatg ggagataaat ctagagcaag agaaactatg atggaattga atatccctgt
    13621 tgttcctggt tcagaatctg ttttgaaaac taaggaagaa gctttagaag ttgcaagaga
    13681 aattggatat cctgttatga taaaagcctc aagtggaggc ggtggaaaag gtatgagaat
    13741 cgttagaaaa gaagaagaac ttttttcaaa ttttgatatg gcaagttcag aagctctagc
    13801 agcattttct aatagtgatt tatatatgga aaagtttata gaaaatccta gacatataga
    13861 agtacaagta tttggagata aacattcaaa tgcaatacat ttaggtgata gagattgttc
    13921 tatgcaaagg cgaaatcaaa aagtaataga agaatcttta agtccatacc tttcagatga
    13981 agaaagacaa aaacttcata aaattgcagt agatatagta aaaggagtag gttatatcgg
    14041 tgcaggtact attgagttta tagttgacaa agataaaaac ttttatttta tagagatgaa
    14101 tacaagaata caagttgaac atccagttac agagatggta acaaatcttg accttataaa
    14161 acttcaaata tctatagcta atggggataa aattcctttt aaacaagaag atattacttt
    14221 tagaggtcat gctattgaat gtagaataaa tgcagaagat tcatcaaaga attttgctcc
    14281 atcaccaggt aagatagaat cattaaattt acctggaggt tttggtgtta gatttgatac
    14341 ttttgtatat gcaggatata ctataccacc tttatatgat tctatgatag gtaagttaat
    14401 atgttgggct gaaactagag aagaatgtat aagtagaata tatagagctc tagacgaaat
    14461 tattgtagaa ggtattaata caaatgtaga atttcaaaag gcacttgtaa caagtgagga
    14521 gtttagagaa gatactcatc ataccaaatt tatagaagat gtctttatga agaaagaatt
    14581 tgctacatta taaggagtaa atagattatg ataaaaaaat ttttatcatc aaaagaatct
    14641 aagtatgtta cgatttcttt agatgacaac tttaaaaaga atagtgtaga tgataaattt
    14701 tggacctatt gcaaaggttg tgatagtcat gtatttagaa aggacataga agaaaattca
    14761 tttgtatgtc caaaatgttc aagacattat ggcttaagag caagaaaaag aataaattta
    14821 ttgattgata aaggaacttt tatggagttt aattctgatg tagaatttca aaatccacta
    14881 aattttccta aatacaaaga aaaagtagac tcttataagg aaaaaacaaa agagtcagaa
    14941 gctgttgtca caggttatgg aagaataaat ggaattaaaa cagttatttg tgtaatgaat
    15001 cctgatttta tgatgggaag tatgggttct attgttgggg aaaaaataac ttattcaata
    15061 gagtatgctg ctgaaaataa tttacctata attatttgtt ctgcttcagg aggagctaga
    15121 atgcaagaag gtatggtatc tcttatgcag atggcaaaga cttctcaagc actttctaag
    15181 cttgaagaaa aatcattgcc atatatatca gttctaactg accctactac tggaggagta
    15241 actgctagtt ttgctatgct tggtgatatt ataatttctg agccaaatac gttgataggt
    15301 tttgcaggtc caagagtaat agagcaaaca ataaatcaaa aacttcctga aggatttcaa
    15361 acttctgagt ttttacttga aaaaggtttt attgacatga tagtagatag aagaaaaatg
    15421 aaggaagtat tatatcaaat tcttgctatg cacaagaaat agggcataaa ggaggaactg
    15481 ttttgattga aaataatagt gaaaaaataa aagtcttaga aaatgatatt aaacaattaa
    15541 taactatttc taaggcaaat aatatagatt taagtgataa aataaattca cttaatgaga
    15601 aattggaaaa gcttaaggaa gatgcttttt cacatctatc agcatatgaa aaggtaacat
    15661 taagtagaga tataaagaga cctacgacat tggaatacat agaacatatt tgttctaatt
    15721 ttttagaact tcatggagat aggcttttta aagatgaccc ttctatggtt ggaggaattg
    15781 gtcagatagg aaagtttaat gttactatag ttggacacca aaaaggaaga gatacaaaag
    15841 aaaatataaa aagaaatttt ggaatgcctc atccagaagg atacagaaaa gcattaagac
    15901 ttatgaaaca agctgaaaaa tttaatagac ctataataac gtttattgat acatcaggag
    15961 ctttttgtgg tctagaagca gaagaaagag gacaaggaga agctattgca agaaacttac
    16021 ttgaaatgag taaactttct gttccagtaa tcacttttgt aattggtgaa ggtggtagtg
    16081 gaggagcttt aggtatcgga gtaggaaatg atgtatgtat gttagaacat tcagtttatt
    16141 ctgtgatttc tccagaaggg ctttctagta ttctatttaa agattcatct aaagctaaag
    16201 aagcttgtga tgttatgaag cttacaagta atgatttata tgatttaaaa ataatagata
    16261 agattataaa agaacctctt ggaggagcac aaaaagatgt agaagctgtt tcaaaagaaa
    16321 taaaagctta cattttagaa agattaaatc attacaaaga tatggataaa gaggaaatta
    16381 tagctcaaag atataaaaaa tttagaaata ttggaaaatg cttatagaat ataaagaaat
    16441 ttgtagtttt ttcataataa aatggttaaa aaaaataaag gttttcttaa aacgattata
    16501 gaagtatatt tttataaaag tatactttaa atgatgattg taattagtaa atggcaatta
    16561 tgatttagta ctttaggtaa tggatacttt aacaaattaa caaggagatg ttgaaatgga
    16621 ggtactaaaa gtttcatcaa aatctaatcc taattctgta gctggagcct tggctggagt
    16681 actaagagaa cgaggcgttg cagaaataca agctatagga gctggagctc taaatcaagc
    16741 gataaaatca attgcaatag caagaggttt tgttgcacct agtggaatgg atttagtttg
    16801 cattcccgca tttactgata tagaaatcga aggtgataag aaaacagcca ttaaacttat
    16861 aattgaacct agataatata tctctttatt agaatttaat taagttttaa aggttggcta
    16921 tagaaatagg acactatagt gaaccttttt tactttatat gttttatttt atgtaacttt
    16981 taatgtataa tcttaagtaa acaaataaga ttggaggaaa tatacattat gaaattaaaa
    17041 ttattggtag aagagtatgc agtatgtaga ttaaataatg acagtaaaat accaacatgg
    17101 atagatacag agaagtttta ctctataact agaactgatg atgaattaag tgttgtatgt
    17161 ttaaataata atattccttc tgatgttaag tcagaaaagg agtggagaat acttaagata
    17221 ctaggtcctt tagacttttc acttgtagga attttatcta agttaagtag tttgttagca
    17281 gacaacaaaa taagcatatt tgcaatttct acttatgata cagactatat cttagtaaaa
    17341 gaaaaggata tagaaaatgc ttgtaaaatt ttaagctgta atggatatga agtagagtag
    17401 ttaaatactt ttacatgagg atttaactta tgatttatat aatcgaaatt ttaaaaataa
    17461 gtgtaataaa tattttttgt ttcataggaa tatttattgt atttggattg atttttagca
    17521 ttacagaaaa tctaaataat aaatttatat tttcatcttt tggaaaaact ggaattattg
    17581 taactggagc tataggaaca tttgttcatg aattgagtca tttgataatg tgtttaattt
    17641 tcatgcataa aataaattca gttaagtttt tcagaccaat agaaagtaaa aatgatggta
    17701 tactaggata tgtaagtcat agctataaaa aagataatct ttatcaaagt ataggaaatt
    17761 tctttatagg aattgcacct attataggtg gaacaataat gattatttta atttttaaga
    17821 tattactgcc agatagttat atgaaagtga ctcaaaatat agatttaaag ttatatgtaa
    17881 gcatgattaa taatttaaat ataaatggtt ttatacacat tattttaggt gatatttcca
    17941 attttataag aataatgttg ttaacaccta gtgtatattc attgaagtat tttatattta
    18001 tgtttttaat gtattctata tctacacata tgtcacttag tagtgctgat ttaaaaaata
    18061 gcttaaatgg cctgagcttt atagttatag ttatttttgt aataagttca attacatact
    18121 tatttggatt tgataacttg aatatatcca gtttaatagt taagtataac atatttataa
    18181 gtttctttat ggcaataggg tttatttttt caatagtaac tctcataata tcctatattt
    18241 taagcttagt cagcccattt aaaaacttta gttaaataaa tacatatttt actaatattt
    18301 ggtttcgtta gaaacatata aaaatattat ataaaaaaga ttctagtaga gcataaaaaa
    18361 tttctcaata tatttatata aatatattga gaaataaaaa agagtactta aattcaacta
    18421 gtctttaaat gtggatttag ggaatagtta gtgataattt catct
//
LOCUS       DAYAPA010000080        18280 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.080, whole genome shotgun sequence.
ACCESSION   DAYAPA010000080 DAYAPA010000000
VERSION     DAYAPA010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18280
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.080"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            194..823
                     /locus_tag="ACGI59_002522"
     CDS             194..823
                     /locus_tag="ACGI59_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HFG9740305.1"
                     /translation="MDKNYTMPIYQKIALDIANKIYTGEIQEDSVLFGRSVLAGKYNV
                     SPETIRRAVKILEDIGVVKSIKGKGVIVLSPDKASSFIKKYRDITNISSYKSTLYNLI
                     DTKSNLENEILDTINKILDYSNRLEIINPLVPVQFTINSNCKYIGQTAAQTKFWQNTG
                     ATIVAIKRGEELIISPGPYIEFLEGDILLVVGDQHIYNSIPMFLYENEK"
     gene            complement(937..2451)
                     /gene="glpK"
                     /locus_tag="ACGI59_002523"
     CDS             complement(937..2451)
                     /gene="glpK"
                     /locus_tag="ACGI59_002523"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFG9740306.1"
                     /translation="MEKKYVMALDQGTTSSRAILFNKKGEIVKIAQKEFNQIYPKAGW
                     VEHDPMEIWGSQSGVMREVIETAGIRPEEIASIGITNQRETTVVWSRYTGKPIYNAIV
                     WQCRRTSEICDELKNKGLEESIKEKTGLLIDAYFSATKVKWILDNVEGAREKAEQGEL
                     LFGTIDTWLIWNLTRGKVHVTDYSNASRTMMYNINDLEWDEDILRELDIPISMLPLVK
                     PSSYVYGHTDERMLSGAKIPIAGCAGDQQAALFGQNCVEEGTAKNTYGTGCFLLMNTG
                     SNIVKSKHGLLTTIAWGVDGKVTYALEGSIFIGGASVQWLRDELKIIESAKDSEMYAN
                     RVEDTNGVYVVPAFTGLGAPYWDMYARGSILGLTRGAKKEHIVRATLESIAYQTKDVL
                     EAMQNDSKLKLKSLKVDGGASNNNFLMQFQSDILNVDIDRPKIVETTALGAAYLAGLS
                     VGFYIGRNEITSKWSVEKEFNPNMSEEKRCKLYKGWKKAVSRALSWEKEDELDI"
     gene            2695..3237
                     /locus_tag="ACGI59_002524"
     CDS             2695..3237
                     /locus_tag="ACGI59_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFG9740307.1"
                     /translation="MPKILENVKEDILKVSRDMILEEDYSNISIRKIAQRCGISAGTV
                     YNYFNSKQEIIEYITKSEWDLLIRRIEYSNKNTEDYIKKLNIIFTEIRNFINKVHNIQ
                     YNDFLNTFETERFFEMKKHKDDFHEQLSNKVYEALEKESFFNNDKLVCNIIIKMFFSY
                     STYLHIEFEDLKPYIEKLIH"
     gene            3242..5743
                     /locus_tag="ACGI59_002525"
     CDS             3242..5743
                     /locus_tag="ACGI59_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFG9740308.1"
                     /translation="MFTLNHNTSIKNKDSNPKIFLVLTLYLLGIFMGAIDTGIVSPAR
                     TIIQNSLGVNEKTGIWMITIYTLTYASVIPISGKLADKIGRKYVYLVSIFLFGSGSLI
                     CGLSSLFSNFYILLIGRVIQAIGGGGIMPIATAEFGTTFPENKRGMALGLVGATYGIA
                     NILGSSIGSTILSIFGTQNWKWLFFVNLPICLIILIGGVFCIKNNKSESKEKIDKLGT
                     LMLICIIVSLLYGLMNIDFFNFKNSIQDISVYPYLLTFIVLIPIFIFIENRAKDPILS
                     FEYFLNPRILIVLILSLIVGIGMMGMVFVPQYAENALKINAGSGGYFVAILGLFAGIA
                     APLSGRLIDKFGAKKILLLGFSVSMIGSLYLVLIALKTNTVFSVCVSLIFMGLGMGFT
                     MGTPLNYMMLSNTRLEESNSALATLSLIRSIGTSISPAIMIGFIAHAGLSVQDNIMDL
                     VGKPSTPKIVQLEELENMIDDLKSDPEMAKNLKNVSIPNMNDTSNINMDMTGGKLPKS
                     LLNKVQSADTTNITNITKEISTTMFDEKVPSIITKIQKNVQKGIDGTQSGINGIEDGE
                     NKLNSGIKGVQTGIDNMVKARAGINQGIDGVKKGIAGVDKGIKGMEQGLEKQDEAIKE
                     LTTAYNKIPTTEKTPTDNENNSNEQNQSNTSNSESDDTKNDSTENKNNNSSENKPNVN
                     EQKESLNAQIQQLKKTRDDLNTKLQKNKTQKNELSKKLRTMESQKKELQSKLNNSISQ
                     KKDMEKSLDTMQQQKKSLQAVLAKTQEVKTEIPKAFDKSKLDYINSIENNRTKIENTL
                     QSTLNSGFKQMYITVFCVNLLAFIILLFYKENKTR"
     gene            6164..6562
                     /locus_tag="ACGI59_002526"
     CDS             6164..6562
                     /locus_tag="ACGI59_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFG9740309.1"
                     /translation="MDTLGKRIAYLRNSKKLTQRKLMDILKFENLGKYETGDRKPNCD
                     ILMSIADYFNVTTDWLLYGKEKVNVNSSVKEDKEDYLHVTNDEMTILNLYRQLNERDK
                     IKIEGILELKISEYKDLKKHSSNNNEDKMV"
     gene            complement(6779..6988)
                     /locus_tag="ACGI59_002527"
     CDS             complement(6779..6988)
                     /locus_tag="ACGI59_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9740310.1"
                     /translation="MCKLDSDGVIECCRAIDDFITALSNIKSLNMERLNTLTKYSSTC
                     SILLKEGNYEGCTIVYRKMLEELKT"
     gene            complement(7020..7229)
                     /locus_tag="ACGI59_002528"
     CDS             complement(7020..7229)
                     /locus_tag="ACGI59_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFG9740311.1"
                     /translation="MDARKKWIPFLGVQVKQRLIELNMTQRELAKKIGVNENYLSAIL
                     NGRRTGKKYKSSIYQLLNIEYSEDD"
     gene            7528..7947
                     /locus_tag="ACGI59_002529"
     CDS             7528..7947
                     /locus_tag="ACGI59_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFG9740312.1"
                     /translation="MDTLGERIVYLRKAKNLKQYELEEMLGCDNLSKFERNIRKPNYE
                     ILKSIAEIFNVSVDWLLNGDNLSHKSDLICDSSSNYPLNSINSNEIKLLNNFRKLNDY
                     DKAKIEGMIELKLHEYEKEKDLGKIEYNKNKDEKIDK"
     gene            8175..8630
                     /locus_tag="ACGI59_002530"
     CDS             8175..8630
                     /locus_tag="ACGI59_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9740313.1"
                     /translation="MGLRDKFAQSFARSKTMSGPEKKANEIMGKLLLKKAILPIVLMF
                     VIIIAGAMLKINSWVTLGINLVIAVGAFFYIRNSSKKYQNFKPYVGNLISLEKKGKKE
                     YVAIIKQGKLPVKLQIAYGGEDLEHVKKNQMVQISYNPDAKIAILVNRQ"
     gene            complement(8851..9111)
                     /locus_tag="ACGI59_002531"
     CDS             complement(8851..9111)
                     /locus_tag="ACGI59_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9740314.1"
                     /translation="MDKLITELSSLGAIGILCALLFKNTMQEKKEDRDMYKKTVENFI
                     ELSTQQQEINKNILVQMGIMKTDVEEIKEDVTGIKGMLQNGV"
     gene            complement(9374..9727)
                     /locus_tag="ACGI59_002532"
     CDS             complement(9374..9727)
                     /locus_tag="ACGI59_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD1375 family protein"
                     /protein_id="HFG9740315.1"
                     /translation="MFKFDKNKIEQIKQGRKVEMQYKDISDISIGQVKQDDDITNNFI
                     ANAEIYEMLLNQSSVNEASNISSFSVRKSGGESGMVEVYVALILRGKKTIEEVPAVIR
                     EQVRIRCKELEIPVE"
     gene            complement(9744..15065)
                     /locus_tag="ACGI59_002533"
     CDS             complement(9744..15065)
                     /locus_tag="ACGI59_002533"
                     /inference="COORDINATES: protein motif:HMM:NF012632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9740316.1"
                     /translation="MKRTKLLQRGNFFGDKNMVVDEFDEGYDNYDFINFFTGCCNYTF
                     GLKNNNILYGCGDNSNFQLGLGEDNTTRKLFTKIPNISTNIKKVACGESHAVILTSDG
                     ELLVAGINTDGQMGLGLEKVGKTVSTFEKVPEIKGVKDIACGLQSTYLLYNDGTLYVA
                     GNNLYGQLGLGTNGASANVNTFIKVDVDNVKAVFSYNKSAFIIKNDNKCYSTGFNNQG
                     QLGLGDKNNRDLFSLVSINDVKTIACGSEHTVLMTYNNDIYGCGKEKCFGNALQSSLF
                     TKIEEVNIKTIACGNGNTMLIDNKGILKVAGNNDIYQLGIANYSENIDNSFIDLKNIV
                     AKNIFIGLSHSILIDSNNDSYCTGDNTYGQLGSFFDDMHIVEFKKMDSEKYSYSNYIN
                     LIKSEDKLTLLKEEMEIKDIELPLDIHSVRDVVFSPYCTLVILGNGDVYGLGNNRYKG
                     MGSDLPSQLNELTKLSISNVKSIVASKNISGGIFYIKNDDTCYYSGPNSNSIAGVLPS
                     NSDIFKKISIDNVKKVVINTDLSNWFSLIVTNNKQIYTSGKSSSYVNGLSNKSASQYT
                     EIGVSNVTDAYSSYNATFIVADEKKVYATGINTNYLLGVNPNDGVNINLTLINNWFYV
                     NPSGASYSTNSCTNDITKINNIIIYEYMAILCTNYGSFLTGYHGTLWTKPSDSTYRVQ
                     QQGITHAGYISNYIYNYYPTRCTQSSYSTNFAYLYYGESTSNLKNVNPDNLLISGGSS
                     YIHQYGRNYLNNQSSNNIAASNINSGPITSDKAIFLYKALLYLSSNTLYGFGNISESA
                     KELDVSDTQDGYNATNYKKVMKNIKNIFIPPYDLSRDKTRFAILTDKSLFICGYNSKG
                     THGISVNSSLNLNNKINYNKKNSSSEISSNIQEIYSHSKSTYLLTNNNMLYSVGLNDV
                     GQLGVGDEINRKVFTKINIDNIKSINVNRFTDNSKHVFAIKNDNTCYAVGLNNSGQLG
                     IGDNVNRNVFTKINVENVKYVAVYGNTSLLLTNDGLLYGAGNNGKGQLGLGDTTSRNI
                     FTRIPINGVRDVYICNDVSIIVKNDNTCYVCGFVNGYFGFTEGSISTFTKINIENVKS
                     VVTAGSEATFFITNDNVIYTTGKKERVFFSTETNDIKGIRVINNIINAKKIVVNGYTS
                     AILTNDNKLFVGGLSGYGSIANNNNTNSVEDVKDVFVTANNTLYIDNNNNLISSGRDT
                     YGISDESYRDMSVPYYKVSIKKDVDTVFSSYNTIFIKDIYGKFYSSTGDNRYNHLGIH
                     HRYDNDKNEALEGSLHSYFKTDNTSDKIVFNKKNEKLVMFNDKYIKTNNKYINYKNIF
                     KDNFKYTSIILPFEVSDIDISKTHSLAVAKDGKLYGIGSNSYKEINQTLEDIELLTLT
                     EVNISDVKKVACGDNYSYIIKTDNTLWSYGKNTEYQLGVGHNNDVRELQKVTGLPSVK
                     DISIYNSMTLVLTNEGELYAQGYNTNGLFGLGESEKDKIIRTFTKVLTNVKEIKSHND
                     DHILVIKNDNSLWITGKNKSMYKISISITDLYEFTKIPIPEHLNDILDIELSDDTIYM
                     ITKVDTSKASIEIVEKSISQVRVVVQDPNNVIEKLEMFINDELISTKTNLEINSIIFE
                     IPQNKIVLGENKILIKASSPTGDLYSSMFIFKSETGLKVKKDSILMINNKVYSIINIT
                     ENNTDLIVTLNEGLKDDMMENNPIYQLINKTKVQVKINKSDLFKDMKLVEIKKSDSSY
                     QEIYELEEANIKSAQPKIIVEKGDKWTAIKRPSMIFRYDAESNEPQA"
     gene            complement(15082..16065)
                     /locus_tag="ACGI59_002534"
     CDS             complement(15082..16065)
                     /locus_tag="ACGI59_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFG9740317.1"
                     /translation="MEEKFYIILTKIGREKIANATALGELVGLTKFQVGDSNGEYYEP
                     TEEQTALKNVVWEGNINSLRIDEKNPNWIVIETILPGTVGGFMIREAAVLDNENNIIA
                     IGKYPETYKPRAEDGSIKDLVVKMILQLSNTSNVTLEVDPTLVFVTQKDIQDLDDKVD
                     KNIKEIKVKIGEELLSTEAKNLSGAINEVVEKIKNISIDDVIGGQIQTELSVLKNSYN
                     KLSEKVLDILIYLELESEIDVDEAGYWYDTLTNAKNIIAIEGLKLDLNRKCITGELGS
                     VTFKNVVLPFNANRVRYIHEMDNNFVETKSNRAYSIGQTDITLNKYSYEIR"
     gene            complement(16066..16764)
                     /locus_tag="ACGI59_002535"
     CDS             complement(16066..16764)
                     /locus_tag="ACGI59_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative phage tail protein"
                     /protein_id="HFG9740318.1"
                     /translation="MKLIDKLPSFDRNYIVEEIQGAYDTELNILKEDIDDTFNQLFVD
                     TATWGLDMWEDILCIEKKELDFDTRRSNIKAKMRSRGTSTIEVIKSICEAYTKSETDI
                     KVYSDEFTFVLSFIANNCDYKTLLDCSDMIERVKPAHLLHYLEPIILDKSMVYCGGGM
                     VCSEEVKVHPYFEPIIKCSAVVNCGAGMLSREEIKVYPLSIKCIENNCKINIAIANDT
                     GVENVVVYPKSEVV"
     gene            complement(16761..17813)
                     /locus_tag="ACGI59_002536"
     CDS             complement(16761..17813)
                     /locus_tag="ACGI59_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFG9740319.1"
                     /translation="MYSDQTYEVIKNRTLENINLDIYKGEGSFLNNMVSGNNLELSKI
                     YLELSKIHKMAFIQDTYNQFLDKRVNEFGVYRKLGTESNGEVEFIGEKGTVINNGTII
                     SYRDLLFVVIKDVTIGSEEGDNSPVQALEVGKKYNLPTNCEFKLVDNISGVTKITNTR
                     SFEGGTDIETDEELKERFYKIQRNQATSGNKAHYEEWALEVDGVYNVKVYPRWDGPGT
                     VKVLIFGKNNQAVDTETIERCQQHIDEEKPIGPTITVVTPLPIEINISAVMKLEDGYT
                     LDNVKESFLESINTYFRDIRGEIIYTKVMGILINTTGVHDLSNLLINGSTDNITINED
                     KIPSVTTVNFSEVENQ"
     gene            complement(17806..18234)
                     /locus_tag="ACGI59_002537"
     CDS             complement(17806..18234)
                     /locus_tag="ACGI59_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HFG9740320.1"
                     /translation="MSTIFPFIGVPEDYILPKTEELPIFREVAWDFEKDEPILEKGDF
                     KIIEKKEALKVWIYKCIKTNRYEHEIYSLEYGTELSELIGQKYTKGLTESEASRFIKE
                     ALLINPYILEVNVKSANFNRDVLSANVKVSTIYGEVEINV"
BASE COUNT         6264 a         2888 c         2110 g         7018 t
ORIGIN      
        1 atatttttat aattaattct ttatatagtt ttaagtataa taaaatcatg tatactaaat
       61 gcatgttaaa atattatttt aaaacatgca caatatttgt acatattgta gacatataaa
      121 cctataaatt atacaatagt attgttaaga ttatatattt ttttaacata aattttgtat
      181 tgggggaaaa acaatggata aaaactatac gatgccaata taccaaaaaa tagcgttgga
      241 tattgcaaat aaaatataca cgggtgaaat acaagaggat tcagtactat ttggacgctc
      301 tgttttggct ggtaaataca atgtatctcc agaaacgata agaagagctg tcaaaatttt
      361 agaggacatt ggcgttgtta aaagcataaa gggtaaaggt gttattgtac tatctccaga
      421 caaagcttct tcttttataa aaaaatatag agatattaca aatatatcat catataaatc
      481 aacactttat aatttaatag atactaaatc taatttagaa aatgaaatat tagatactat
      541 aaataaaatt ttagactatt ctaacagact tgaaataatc aatcctttag tgcccgttca
      601 gttcaccata aattctaatt gcaaatatat tggtcaaaca gctgcacaaa ctaaattttg
      661 gcaaaataca ggtgctacaa tagttgcaat taaaagaggt gaagaactta taatatctcc
      721 tggaccatat attgaatttt tagaaggaga cattctctta gtagttggtg accaacacat
      781 ttacaattct ataccaatgt ttttatatga aaacgaaaaa taaattatat aatttagtgt
      841 gattatataa aaatgcaaga ttaacaacaa aaaaagagct tcatatttta cataaagctc
      901 tttttaaatt caaataaatt taactttgat taatttttaa atatccaatt catcttcttt
      961 ttcccagctt aaagctctcg atacagcttt tttccatcct ttatataatt tacatctctt
     1021 ctcttcactc atatttggat taaactcttt ttctactgac cactttgaag taatttcatt
     1081 tcttccaata tagaatccaa cagacaaccc tgctaaataa gcagctccta aagctgtagt
     1141 ttctactatt ttgggtctat ctatatctac atttaatata tctgattgaa attgcattaa
     1201 aaaattatta ttacttgctc caccatcaac ttttaatgat tttagtttta attttgaatc
     1261 gttttgcatt gcttctaaaa catcctttgt ctgataagca attgattcca atgtggctct
     1321 tactatatgt tctttttttg ctcctcttgt aagtcctaaa attgaacccc tagcatacat
     1381 atcccaatat ggagctccaa gacctgtaaa tgctggcact acatatacac cattagtatc
     1441 ctctacccta tttgcataca tttcactatc ttttgcagat tcaattatct ttaattcatc
     1501 tctaagccat tgaacagatg ctcctccaat aaatatactt ccttctaatg catatgttac
     1561 ttttccatca actccccaag caattgttgt aagtaaccca tgcttagatt taactatgtt
     1621 actgcctgta ttcataagca agaaacaacc cgttccataa gtattttttg cagtaccttc
     1681 ttcaacacaa ttttgaccaa ataatgctgc ttgttggtct ccagcacatc ccgcaatagg
     1741 tattttagct cctgataaca tgcgctcatc tgtatgacca taaacatagc tagaaggttt
     1801 tactaatggc aacatgctta ttggtatgtc caattctcgt aatatatcct catcccattc
     1861 taaatcattt atattgtaca tcattgttct agatgcattt gagtaatctg ttacatgtac
     1921 ttttccacga gttaaattcc atattaacca tgtatctata gttccaaata ataactctcc
     1981 ttgttctgct ttttccctag ctccttctac attatctaat atccatttta cttttgttgc
     2041 tgaaaagtat gcatctatta aaagacctgt tttttctttt attgattctt ccaatccttt
     2101 atttttaagt tcatcacata tttctgacgt tctcctacac tgccatacta ttgcgttata
     2161 aataggttta ccagtatacc tactccaaac aaccgtagtc tctctttgat tagttatccc
     2221 tattgaagca atttcttctg gtcttatacc tgctgtttct ataacttctc tcattacccc
     2281 actttgtgac ccccatattt ccatagggtc atgctctacc catccagctt ttggataaat
     2341 ttgattgaac tctttttgtg caattttcac tatttcgcct tttttattaa atagtatagc
     2401 tcttgaactg gttgttcctt ggtctaatgc cattacatac ttcttttcca tcaccatatt
     2461 ctccttatca tataatattt aatttattaa aaagaaaaat ttaatatcac atatttcaat
     2521 aaataatcat acttaagtac aaatttaata taatagatat ttagtaagaa gacacttatt
     2581 ttctatcata tctatataat aatattatac attatagctt acatattgat taataaattt
     2641 taaaggagta taataaacag tgttcataat aattttaata aaagaggtgg taatatgcca
     2701 aagattttag aaaatgtaaa agaagatata ttaaaagtgt ctcgggatat gattttagaa
     2761 gaagattact cgaatattag cataaggaaa atagctcaaa ggtgtggtat aagtgcaggt
     2821 acagtgtata attactttaa ttcaaaacaa gaaataatag aatatatcac aaaatctgaa
     2881 tgggatttat tgataaggcg aattgaatac tccaataaaa acactgaaga ttacataaaa
     2941 aaactaaaca ttatttttac tgaaataaga aattttataa acaaagttca taatatccaa
     3001 tacaatgact ttttaaacac ttttgaaaca gagagatttt ttgaaatgaa aaaacacaaa
     3061 gacgactttc atgaacagct ttcaaataaa gtatatgaag ctttagaaaa agaaagtttt
     3121 tttaataatg acaaattagt atgcaatatc ataataaaaa tgtttttttc atattcaaca
     3181 tatcttcata tagaatttga agatttaaaa ccctatattg aaaaattaat acattaaggt
     3241 ggtgtttacc ttgaatcata atactagtat taagaacaag gacagtaatc caaaaatatt
     3301 tttagtactt acgctttatc tattaggtat atttatggga gctattgata ctggaatagt
     3361 atcaccagca agaacgatta tacaaaatag ccttggagta aatgaaaaaa cgggaatttg
     3421 gatgattacc atatacacat taacatacgc ttctgtaatt cctatctctg gaaaattagc
     3481 agataaaata ggaagaaaat atgtgtatct agttagtata tttctatttg gtagtggttc
     3541 attaatatgt ggattatcat cactattttc caatttctat attttattaa ttggtcgtgt
     3601 tattcaagca attggtggtg gtggaatcat gccaattgct acagcagaat ttggaactac
     3661 cttcccagaa aataaaagag gcatggcttt aggtttagtt ggggcaacat atggtattgc
     3721 caatatttta ggttcaagca ttggtagcac aattctaagt atctttggaa ctcaaaattg
     3781 gaagtggcta ttttttgtaa atcttccaat ttgtttaatc atacttattg gtggagtttt
     3841 ctgtattaaa aacaataagt ctgaatctaa agaaaaaata gataagcttg gtacattgat
     3901 gttgatatgt ataatcgtat cactattata tggtcttatg aatatagact tttttaattt
     3961 taaaaattct atacaagata tatcagttta tccatactta ttgacattta ttgtacttat
     4021 acctattttc atatttattg aaaatagagc taaagaccct attttaagct ttgaatattt
     4081 tttaaatccg agaatattga tagtacttat tttatcttta attgttggta ttggaatgat
     4141 gggtatggta ttcgttccac agtatgctga aaatgcatta aagataaatg ctggttctgg
     4201 gggatatttt gtagctatat tgggattatt tgcaggtatt gctgctcctt taagcggaag
     4261 acttattgac aagtttgggg caaaaaaaat actattacta ggattttctg tatctatgat
     4321 aggctctctt tacctagttt taattgctct aaaaaccaat actgtattta gtgtatgtgt
     4381 aagtttaata tttatgggat taggaatggg atttactatg ggaactcctc taaactatat
     4441 gatgttatca aatacaagat tagaagaatc taattcagca cttgcaacat tatccttgat
     4501 acgctcaatt ggtacttcaa tttctccagc tataatgatt ggatttatag cacatgcagg
     4561 attatctgtg caagataata ttatggactt agttggaaag ccctcaactc ctaaaatagt
     4621 tcaacttgaa gaacttgaaa acatgataga tgacttaaaa tcagaccctg aaatggcaaa
     4681 aaatttaaaa aatgtctcta tcccaaatat gaatgatact tcaaatataa atatggacat
     4741 gacaggtggc aaacttccaa aatccttact taataaagtt cagtcagcag atacaaccaa
     4801 cattaccaat ataacaaaag aaatatcaac aacaatgttt gatgaaaaag ttccatctat
     4861 aattactaaa atacagaaaa atgtacaaaa aggaatagat ggaacacaaa gtggaataaa
     4921 cggtattgaa gatggtgaaa ataaactaaa tagtggtatt aaaggagttc aaactggtat
     4981 tgacaatatg gtaaaagcta gagctggtat taatcaagga atagatggtg taaaaaaagg
     5041 tattgctggt gttgataaag gtataaaagg tatggaacaa ggtcttgaaa aacaagatga
     5101 ggccatcaag gaacttacaa ctgcttataa taaaatacct actacagaaa aaactcctac
     5161 tgataacgaa aataactcaa atgaacaaaa ccagagtaat accagtaatt ctgaatcaga
     5221 cgacactaag aatgattcta cagaaaataa gaataacaat tccagcgaga ataaaccaaa
     5281 tgtaaatgaa caaaaagaaa gtttaaatgc tcaaatacag caactaaaaa agacaagaga
     5341 tgacttaaat acaaagttac aaaaaaataa aactcaaaag aacgaattaa gtaaaaaact
     5401 aagaacaatg gaatctcaaa agaaagagct acaaagcaag ttaaataatt ctataagcca
     5461 aaagaaagac atggaaaaaa gccttgatac aatgcaacag caaaaaaaat cactccaagc
     5521 agttttagca aagacacaag aggtaaaaac tgaaatacca aaggcttttg ataaatcaaa
     5581 attggattat ataaattcca tagaaaataa tagaacaaaa atagaaaaca cactacaatc
     5641 tactctaaac tcaggattta aacagatgta tataacagta ttctgtgtaa atctactagc
     5701 atttataata ttattgttct ataaagaaaa taaaactaga taaaagagtg ttatagtatt
     5761 atattgtaaa tttatcttat tcttaaatat tttattctaa agaataaatt cataaaattt
     5821 ttgtaattga ttaatataaa ttacaatata atactatttt tctcttaatc tttttctctt
     5881 ttacagacaa tcaaaaagca atcttagttt atcgaaataa atttatacaa tctattcata
     5941 caccccttta catttatata acaattaaac ctattgttat atatacaaat caaaataaac
     6001 catatatttt caactataat atgttaaaat catattatga gcatattaat ttaagcaaaa
     6061 tgtcatttag actttaattt agtagtttta tattataaat ttatatattt ttaattttaa
     6121 atgtcattta gacattactc ataaaaagaa aggagtgtat tttgtggata ctttaggaaa
     6181 aagaattgct tatttgagaa actccaaaaa acttactcag cgcaaactta tggatatttt
     6241 aaaatttgaa aacttaggca agtatgaaac aggagataga aaacctaatt gtgatatact
     6301 aatgtcaata gctgattatt ttaatgttac gactgactgg cttctctatg gtaaagagaa
     6361 ggtaaatgtc aatagtagtg taaaagaaga taaagaagat tacttacatg ttactaatga
     6421 tgaaatgacg atattaaatc tatacagaca actaaacgaa cgtgataaaa taaaaataga
     6481 aggaatactt gaacttaaga tatctgaata taaagactta aaaaagcatt cctctaataa
     6541 taatgaagat aaaatggttt aaacatacat aatttgatac gattttacat catctattaa
     6601 ccttcttttt ttgtagtata ttactaaaat atggatattg gattatttaa aaacaagtat
     6661 agtctaattt caagcacatt tataatattt agattatctt atccatattt tagtttaagt
     6721 actttgaaaa atataataca tctctatgta taacttatat tcctaagaaa tgcattactc
     6781 atgtttttaa ttcttccaac atctttctat acacaattgt acatccttca taattcccct
     6841 ctttaagaag gattgaacat gtactagaat atttagttaa agtatttaat ctttccatat
     6901 ttaagctttt tatattactg agtgctgtaa taaaatcatc aattgctcta caacactcta
     6961 taaccccatc tgaatccaat ttacacacaa gaatattcac ctactttata tactatttat
     7021 taatcatctt ctgaatattc tatattaagt aattgataaa ttgatgattt atatttttta
     7081 cctgttcttc ttccatttaa aatagctgac aaatagtttt cattaacacc tattttcttc
     7141 gctaattccc tttgagtcat atttaattca ataagtcttt gcttgacttg cactcccaaa
     7201 aaaggtatcc atttttttcg agcatccatt tatcaacttc ctttctaagt gatttatatt
     7261 tacttttata ttattttagc ttacacaccc cctctattta gtatatattt ttaatatgta
     7321 cacatattaa gtatataaat ttatttaata ctaaataaga ttttttgatt actttgtgct
     7381 aaaattagga tgtaaattcg tatcattaag ttaattataa agtcattttg accttaagtc
     7441 aatgcttttt atgtcatttt gaccttaaat ttgaaactat ttacatctta aaaagtcttt
     7501 ttgacattaa ttgaatggag agtgtttatg gatactttag gagaaagaat tgtttatttg
     7561 agaaaagcaa aaaaccttaa acaatatgaa ctagaagaaa tgttgggctg tgacaactta
     7621 agtaaatttg agagaaacat tagaaaacca aattatgaaa tattaaaatc tatagctgag
     7681 atatttaatg tttcagtaga ctggctatta aatggagata atctatcaca taaatcagat
     7741 ttaatttgcg attcctcctc aaactatcca cttaatagca taaactctaa tgaaataaag
     7801 cttttaaaca attttagaaa gttaaatgat tatgataaag ccaaaataga aggtatgatt
     7861 gaattaaaac ttcatgaata tgaaaaagag aaggatttag gaaaaattga gtataacaaa
     7921 aataaagatg agaaaataga taaataattt ttatgttgct tttgatacga ttttacatca
     7981 tttctttgtt ctaatatttt tatttcattt attttctttt ttataatttt atgataaaat
     8041 aattagtata ttttgcttta cttgaatgta attatttata caaaagtcaa acataatata
     8101 aatgtagtaa atttttagtt tcaagtaata tctcgttatt ataactaaat atttattaaa
     8161 attaggagga taaaatgggc ttaagagaca aattcgcgca atcatttgct agatcaaaaa
     8221 ctatgagtgg ccctgagaaa aaggctaatg aaataatggg taaattgctt cttaagaaag
     8281 ctatattacc tatcgtttta atgttcgtga taataatcgc tggagctatg cttaaaatca
     8341 attcttgggt tacacttggc ataaatttag ttattgctgt tggtgctttc ttctacataa
     8401 gaaattctag taaaaagtat cagaacttta aaccttatgt tggcaatttg attagtttag
     8461 agaaaaaagg taaaaaagag tacgttgcta ttataaaaca aggtaagtta ccagttaagt
     8521 tacaaatagc ttatggtggt gaagaccttg aacatgttaa gaaaaatcaa atggttcaaa
     8581 taagttataa tccagatgct aaaatagcta ttttggttaa tagacaataa tttaataaaa
     8641 aaaaggagtt gtcttaaaat tgattttttc taatcaaatt taagctcaac tcctttttta
     8701 atttttatat attattttta tatattattt ttattacttt ttttaattaa tatttttaaa
     8761 tatcaaatcc ttctcaataa aatagggtac tatctacaat actactttat cttttcctaa
     8821 caatatataa tctggtgcta ctctttcatg ttatacaccg ttttgtaaca taccttttat
     8881 accagtaaca tcttccttaa tttcctctac atctgttttc attataccca tttgaacaag
     8941 tatatttttg tttatttctt gttgttgtgt agataattct ataaaatttt ctacagtttt
     9001 tttatacatg tctctatctt cttttttctc ctgcatagta tttttaaata atagagcaca
     9061 tagtatacct attgccccta gactactcaa ttcggttatt aatttatcca ttatagcctc
     9121 ctattgtcta aattttttat acttatagct ttaatttttg ttatattctt acatcatgca
     9181 atattatatt ttcaggatta tcaatttaat cttctgtact ttaaattgta ggtgcaattt
     9241 atagattgaa taaataataa atatatatac aatttctaat ataacaaaaa ataagtacaa
     9301 ggcttactta ttaaagttaa gaccttgtac tttacataaa aaaaataact aatacatagt
     9361 tattctaaat ttactattca actggtattt ctaattcttt gcatctaatt ctaacttgct
     9421 ctctaattac tgctggtact tcttctattg tttttttgcc tcttaaaatt aaagctacat
     9481 atacttctac cattccactc tcacctccag attttcttac actaaaagag cttatattac
     9541 ttgcttcatt gacagaactt tgatttaaca acatctcata tatttctgca tttgctataa
     9601 aattatttgt tatatcatca tcttgcttta cttgacctat acttatgtct gaaatgtctt
     9661 tatactgcat ttctactttt ctaccttgtt tgatttgttc tattttattt ttatcgaatt
     9721 taaacaattt tcacacctcc attttaagct tgtggctcgt tgctttcagc atcatatcta
     9781 aaaatcatag atggacgttt tatagctgtc catttatctc ctttttctac tatgatttta
     9841 ggctgagcac tttttatgtt ggcttcttct aattcataga tttcttggta acttgagtct
     9901 gattttttga tttcaactag tttcatgtct ttgaataagt cagatttatt tatttttact
     9961 tgaactttag ttttatttat taattgatat ataggattgt tttccatcat gtcatccttt
    10021 aatccctcat ttaatgttac tattaaatca gtgttatttt cagtaatgtt tatgattgaa
    10081 tatactttat tgtttatcat taaaatagaa tcctttttta ctttaagccc tgtttctgat
    10141 ttaaatataa acatacttga atataaatcg cctgtaggac tactggcttt aatcagtatc
    10201 ttattttctc ctaatactat tttattttgt ggtatctcaa atataatgct atttatttcc
    10261 aaattagtct tagtagatat taattcatca tttataaaca tttcaagttt ttctataaca
    10321 ttattagggt cttgtactac aactctcact tgagatattg atttttcaac tatttctata
    10381 gatgctttac ttgtatctac ttttgttatc atgtatattg tatcatctga aagctctata
    10441 tctaaaatat catttagatg ttcaggaatt ggtattttag taaattcata taaatcagta
    10501 attgatatag atattttata catagattta tttttaccag ttatccatag actattatca
    10561 tttttaatta ctagtatgtg gtcatcatta tgtgacttaa tttctttaac attagttaat
    10621 actttagtaa aagttcttat tatcttatct ttttcacttt ctcctagtcc aaataatcca
    10681 tttgtattgt acccttgagc gtacaactct ccctcattag ttaaaacaag tgtcattgag
    10741 ttatatatac ttatatcttt aacagaaggt aatccagtaa ccttttgtaa ctctcttaca
    10801 tcattattgt ggccaactcc caattggtac tcagtattct ttccatatga ccatagagta
    10861 ttatctgtct taataatata ggagtagtta tctccacaag caactttttt gacatctgat
    10921 atatttactt cagtaagagt taataattct atatcttcaa gggtttgatt aatttcttta
    10981 tatgaattac ttcctattcc atataacttg ccatccttag caacagccaa tgaatgtgtt
    11041 tttgatatat caatatcaga tacctcaaat ggcaatatta ttgaagtata cttaaaatta
    11101 tctttaaata tgtttttata atttatatac ttattatttg tttttatata cttatcatta
    11161 aacattacta gcttttcatt tttcttatta aaaactattt tatctgatgt gttatctgtt
    11221 ttaaaatatg aatgtaggga accttcaaga gcttcatttt tatcattatc atatctatgg
    11281 tgaataccta aatgattata tctattatct cctgttgaag aataaaattt tccatatata
    11341 tctttaataa atatagtatt gtaactagaa aatacagtat caacatcttt ctttatagat
    11401 actttataat atggaactga catatcccta taagattcat cagatatacc ataagtatct
    11461 ctacctgatg atatcaaatt gttattatta tctatataaa gtgtattatt agctgttaca
    11521 aaaacatctt taacatcttc cacactattt gtattattat tatttgctat acttccatat
    11581 ccactaagac ctccaacaaa tagtttattg tcatttgtta aaatggctga agtatatcca
    11641 ttaactacta ttttttttgc atttataata ttattaatta ctcgtatacc ctttatatca
    11701 ttagtttctg ttgaaaagaa taccctctct tttttccctg tagtataaat aacattgtca
    11761 tttgttataa aaaatgtagc ttcacttcct gctgttacaa cagattttac attctcaata
    11821 tttatttttg taaatgtact tatacttcct tcagtaaacc caaaatatcc atttacaaat
    11881 ccacatacat aacatgtatt atcattttta acaattattg atacatcatt acatatatac
    11941 acatctctaa ctccatttat aggtatacgt gtaaatatat tcctacttgt agtatcaccc
    12001 aatcctaact gtccttttcc attattacct gctccatata aaagaccatc attagttaat
    12061 aatagagatg tgtttccata tacagctaca tattttacat tttcaacatt tattttagta
    12121 aatacatttc tatttacatt atctcctatt cctaactgac cagaattatt taaaccaaca
    12181 gcataacagg tattatcatt ttttatcgca aatacatgtt tactattgtc agtaaatcta
    12241 tttacattta tagattttat attatcaata tttattttag taaatacctt tctatttatc
    12301 tcatctccca ctcctaattg acctacatca tttaaaccaa cactgtaaag catattatta
    12361 ttagttaata aatatgtaga cttcgaatgg ctatatattt cttgtatatt agaagatatt
    12421 tcactactgc tattcttttt attgtaattt atcttattat ttaaatttaa actactatta
    12481 actgatatac catgcgtacc cttagagtta tatccacata taaataagct cttatcagtt
    12541 aatattgcaa atctagtttt atctctactt aaatcataag gaggtataaa tatatttttt
    12601 atatttttca ttaccttttt ataattagtg gcattatatc catcttgtgt atctgaaaca
    12661 tctagttctt ttgcactttc agatatattc ccaaaaccat ataacgtgtt agaagataaa
    12721 tacaatagag ctttatataa aaatatggct ttatcagagg taataggacc tgaatttata
    12781 ttagatgctg caatattatt agatgattga ttgttaagat aattccttcc atattgatgt
    12841 atataagatg aacctccaga aataagtaaa ttatctggat ttacattttt taaattactt
    12901 gttgattctc cataatacaa ataggcaaaa tttgtagaat atgatgattg tgtacacctt
    12961 gtgggataat agttatatat gtaattacta atatatccag catgtgtaat tccttgttgt
    13021 tgaactctat aagtagaatc acttggcttt gtccataaag taccatggta tccagttaaa
    13081 aaagaaccat aatttgtaca taatatagcc atatattcat atataataat attattaatt
    13141 ttagtaatat catttgtaca agaatttgta ctataacttg ctcctgaagg atttacataa
    13201 aaccagttat ttattaatgt taaatttata ttgactccat cattaggatt aacacccaat
    13261 aaatagtttg tatttatacc agttgcatat accttttttt cgtcagccac aataaatgtt
    13321 gcattatatg aactatatgc atctgttaca ttgctaacac caatctcagt atattgactt
    13381 gctgatttat tactaagtcc attaacataa cttgaactct ttccagaagt atatatttgc
    13441 ttattattag ttacaattaa tgaaaaccag tttgataaat cagtatttat aacaactttt
    13501 tttacattat ctatagatat tttcttaaat atatctgaat tagaaggaag aacacctgct
    13561 attgagttac tatttggccc agaataataa caagtgtcat catttttaat gtagaatatt
    13621 cctccagaaa tattttttga tgctactata gactttacat tagagatact taattttgtc
    13681 aactcattta attgacttgg taagtcagaa cccattcctt tgtatctatt atttcctaga
    13741 ccatatacat ctccattccc taaaataacc agagtacaat aaggactaaa aacgacatct
    13801 cttacagaat gtatatctag tggaagttca atatccttta tttccatttc ttcttttaat
    13861 aaagttaatt tatcctcaga tttaattaaa tttatataat tactataact atatttttca
    13921 ctatccattt tcttaaattc tacaatgtgc atatcatcaa aaaacgaacc taattgtcca
    13981 taagtattat ctcctgtaca atatgaatca ttatttgaat caattagtat gctatgtgat
    14041 aaaccaatga aaatattctt agctacaata ttttttaaat ctataaatga attatctata
    14101 ttctcagagt aatttgctat acctaactga tatatatcat tatttccagc aaccttcaaa
    14161 atacctttat tatctataag catagtatta ccattaccac atgcaatagt ttttatattt
    14221 acttcttcta tcttagtaaa tagtgatgat tgaagtgcat ttccaaaaca tttttccttt
    14281 ccacaaccat atatatcatt attatacgtc attaacacag tgtgttcaga accacaagct
    14341 atagtcttaa catcattaat agaaactaag ctaaataaat ctctattatt cttatctcct
    14401 aaacctagtt gaccttgatt attaaaacca gtagaataac atttattgtc attctttatt
    14461 ataaaagctg atttattata tgagaataca gcctttacat tatcaacatc tacttttata
    14521 aatgtattta catttgcaga tgctccatta gttcctagac ctaattgacc atacaaatta
    14581 tttccagcaa catataaagt tccatcattg tataaaagat atgttgattg aagtccacat
    14641 gcaatatcct ttacgccttt tatttctgga accttctcaa atgtggaaac tgtttttcct
    14701 actttttcta atcccaatcc catttgacca tctgtattta tacctgcgac aagtaattct
    14761 ccatctgaag taagtataac tgcatgagat tctccacatg caactttttt aatattagta
    14821 gatatatttg gtattttcgt aaataatttt cttgttgtat tgtcttctcc caatccaagt
    14881 tgaaagttac tattatctcc acatccatac aagatattat tattttttag accaaatgta
    14941 tagttacaac atccagtaaa aaaattaata aagtcataat tatcataccc ttcatcaaat
    15001 tcatcaacta ccatattttt atcgccaaag aaattacctc tttgaagtag tttagttctt
    15061 ttcattataa aaacctccta attatcttat ttcatacgaa tatttattta aagttatatc
    15121 tgtctggcca attgaatatg ccctattaga ttttgtttca acaaagttat tatccatttc
    15181 atgtatatat ctaactctat ttgcattaaa tggtagaacc acattcttaa atgtaacact
    15241 accaagttct ccagttatac actttctatt taaatctaac ttaaggcctt ctatagctat
    15301 tatgttttta gcattagtta aggtatcata ccaatatcca gcttcatcta catctatttc
    15361 tgactctaat tctaagtata ttaaaatatc taatactttt tcagataatt tattgtaact
    15421 attttttaat acagatagtt cagtttgtat ttgacctcct attacatcat caatagatat
    15481 atttttaatt ttttctacta cctcatttat agctcctgat aagtttttag cttctgtaga
    15541 taagagttct tctccaattt ttacttttat ttcttttata tttttatcaa ccttatcatc
    15601 taaatcttga atatcctttt gagttacaaa aaccaacgtc gggtctactt ctaatgtaac
    15661 atttgaagta ttggacaatt gtaaaatcat ttttacaacc aaatctttaa tactgccatc
    15721 ttcagcacgt ggcttatacg tctctggata cttacctata gctattatat tattctcatt
    15781 atccagaaca gcagcttctc ttatcataaa tccaccaact gttcctggta aaatagtctc
    15841 tataactatc caattaggat ttttttcatc aattcttaga gaatttatat ttccttccca
    15901 aactacattc tttaaagcag tttgttcctc tgttggctca taatattctc cattactatc
    15961 tccaacttga aacttggtta atccaacaag ctctcctagt gcagttgcat ttgctatttt
    16021 ttctctacca attttggtta atattatata aaatttttct tccaattata ccacctccga
    16081 tttaggataa actactacat tttctacgcc tgtatcattt gcaatagcta tattaatctt
    16141 acaattattt tcaatgcatt taatgcttaa aggataaacc tttatttctt ctctacttaa
    16201 cattccagct ccacagttta caacagcgct acattttata attggttcaa agtatggatg
    16261 aacttttact tcttcactac ataccatacc tccaccacaa tagaccatac ttttatctag
    16321 tattattggt tctaaatagt gtaataagtg agcaggtttt actctttcaa tcatatcgct
    16381 acaatctaaa agagttttat agtcacaatt atttgctata aaactcaata cgaatgtaaa
    16441 ttcatcacta taaactttta tatctgtttc tgattttgta tatgcctcac atatactttt
    16501 tataacttca atagtactag tacctctgct tctcatttta gcttttatat tgctacgtct
    16561 tgtgtcaaaa tcaagttctt ttttttcaat gcagagtatg tcttcccaca tatctaatcc
    16621 ccatgttgca gtgtcaacaa ataattggtt aaaggtatca tcaatatctt ctttaagaat
    16681 atttaattct gtatcgtatg caccttgtat ctcctctaca atgtaatttc tatcaaatga
    16741 tggtagttta tcaattagct tcattgattt tccacctcac taaaattaac agttgttaca
    16801 ctaggtattt tatcttcatt aatagttata ttatctgtac ttccatttat aagtaggtta
    16861 cttaaatcgt gtacaccagt agtatttata agtattccca ttacttttgt atagattatc
    16921 tctcctctaa tatctctaaa gtatgtattt atactttcta ggaaagattc ttttacattg
    16981 tctaatgtat atccatcttc taatttcatt actgcactta tatttatttc tattggtaat
    17041 ggtgtcacaa ctgttatagt tggtccaata ggcttctctt catctatatg ttgctgacac
    17101 ctttcaattg tttctgtatc aacagcttga ttatttttcc caaatatcaa gaccttaact
    17161 gttcccggac catcccatct tggataaacc ttaacattat agactccatc tacttccaaa
    17221 gcccattctt catagtgagc tttatttcca cttgtagctt gatttctttg gattttataa
    17281 aatctttctt ttagttcttc atctgtctct atatctgtac caccttcaaa acttcttgtg
    17341 ttagtaatct ttgttactcc agatatatta tcaactagtt taaattcaca atttgtaggt
    17401 aaattatatt tcttaccaac ttccagagct tgaactgggc tattgtcacc ttcttcacta
    17461 ccaatagtta catcttttat tactacaaat agtaaatctc tatatgatat tattgtgcca
    17521 ttatttatta cagttccttt ctctccaata aattcaactt ctccatttga ctctgtacct
    17581 aactttctat atacaccaaa ttcattgact cttttatcga gaaactggtt atatgtgtct
    17641 tgtataaaag ccattttatg tatctttgaa agttctagat atatctttga aagttctaga
    17701 ttatttccag ataccatgtt gtttagaaaa gaaccttctc ctttataaat atcaagatta
    17761 atattttcaa gagttctatt ttttattact tcatatgtct ggtcactata catttatttc
    17821 cacctcccca tagatagtgg atacttttac atttgcactc aatacgtctc tgttaaagtt
    17881 agcacttttt acgtttactt ctaatatata tggatttatt agaagggcct cttttatgaa
    17941 tctactagct tcactttctg taagaccttt tgtatatttt tgtcctatta gttctgaaag
    18001 ctctgtccca tattctaaag agtatatctc atgttcatat ctatttgtct ttatacactt
    18061 gtatatccaa acttttaagg cttctttttt ttcaattatt ttaaagtcac ctttttctaa
    18121 aataggttca tctttttcaa aatcccatgc cacttcacga aagattggca attcttctgt
    18181 tttaggtaag atataatcct ctgggacacc tataaaagga aatattgtac tcattataag
    18241 ctcaccaact tacttacaac agcaaatttt tcgcctattc
//
LOCUS       DAYAPA010000081        18118 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.081, whole genome shotgun sequence.
ACCESSION   DAYAPA010000081 DAYAPA010000000
VERSION     DAYAPA010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18118
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.081"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            171..689
                     /locus_tag="ACGI59_002538"
                     /pseudo
     CDS             171..689
                     /locus_tag="ACGI59_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
     gene            1121..1375
                     /locus_tag="ACGI59_002539"
     CDS             1121..1375
                     /locus_tag="ACGI59_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9740321.1"
                     /translation="MENKDIKLTICIFAFVIGIIVLFCCIGLGQFNMDCIIKQNGGSI
                     ATEQYNIYLEQSISQYRNFGSILVLLGGIGILFDKSVRKY"
     gene            1411..1734
                     /locus_tag="ACGI59_002540"
     CDS             1411..1734
                     /locus_tag="ACGI59_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6054 family protein"
                     /protein_id="HFG9740322.1"
                     /translation="MARDVFSVKMTPLEAQKMVEKNLEADLVSVDIYNLENNKSIIVT
                     IFEKYYARSNSDAGLVVICENTTGKTLVKITSTGSAIGLFQIDWGTGNNLIKRVKKIL
                     IDYII"
     gene            2008..2160
                     /locus_tag="ACGI59_002541"
     CDS             2008..2160
                     /locus_tag="ACGI59_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9740323.1"
                     /translation="MDNFLFNVLASVTASYIVYLISKLIKKVKSHSTAKSDLKIDINF
                     KYHRKK"
     gene            2429..3277
                     /locus_tag="ACGI59_002542"
     CDS             2429..3277
                     /locus_tag="ACGI59_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HFG9740324.1"
                     /translation="MWILVPIITIVLLIIAVSSMQYILVMIAFLLIIYSFIEKKIVMG
                     LVSVLFFTYSIYLCATWEDKSLIADNKVETVRAQRETVEREKEMERRRIQEEVDKERY
                     IEKHGMEISENDLKVKLEALVPQEYKGKKYELKVGKFKRYSMYFDLTVQNEKFSNSEE
                     CKKFVKEIANDLKKIKISKAYFKFHSKDDGGIYNSVYIDYFRNIQNNVDNVENLEFNE
                     FELKTEEEEKREQEKIEQEKNSYNNYIQNRVVDPLDRIKKLKELLDSGAITQEEYNKK
                     KKELLE"
     repeat_region   3544..4359
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=3544..3572
                     /rpt_unit_seq="gttttagattaactatatggaatgtaaat"
     gene            4514..4702
                     /gene="srl"
                     /locus_tag="ACGI59_002543"
     CDS             4514..4702
                     /gene="srl"
                     /locus_tag="ACGI59_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor Srl"
                     /protein_id="HFG9740325.1"
                     /translation="MNKIATKSRAEYMKNCRKDKRGFSVLLDKEKLDKFDEVLEEKNL
                     TKKEWLEEKIDEELEQKE"
     gene            4822..5187
                     /locus_tag="ACGI59_002544"
     CDS             4822..5187
                     /locus_tag="ACGI59_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HFG9740326.1"
                     /translation="MKNELMMFEGKEIEVFEFEGRILFNSKDVANCLDIKNVNENIIL
                     MNEKQVVKLRNSDISNTDIRKLNNAGENFLTESGVYKLIFKSRKEEAERFQDWISDEV
                     LPSIRQTGAYITNNVIPKS"
     gene            5240..5635
                     /locus_tag="ACGI59_002545"
     CDS             5240..5635
                     /locus_tag="ACGI59_002545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9740327.1"
                     /translation="MLKELLDDAGFDNKSKLLTAKTLYKKAEIDLPIEINEEEHYFDT
                     KQIASKLKIYSKSNKPAQMAVCEIIKKIDLEDGEVKGVWEINGSWTGTVNKYTKSVID
                     KVRTWIEENNRPTKIAGEKKNYYVFYKIE"
     gene            5687..5848
                     /locus_tag="ACGI59_002546"
     CDS             5687..5848
                     /locus_tag="ACGI59_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9740328.1"
                     /translation="MCENLLDNMYIEKRKEEYRIKLLKIRETDIDIYNKLESIVYKLS
                     EKKLEKNNK"
     gene            complement(6176..6640)
                     /locus_tag="ACGI59_002547"
     CDS             complement(6176..6640)
                     /locus_tag="ACGI59_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9740329.1"
                     /translation="MDFGEVKSELSDKDLKDNEEALNKYKKYYLKVYKYYSEMDSIKS
                     IQDIEIEQSRLKGQLQKNSNPIFALYISIMAVVFSTTINLTFDLSDITNYFIRMIMTL
                     LFIVLLIFLVNKGILPIAIPYRNNIQFYTTALDVLEDMKNKILRERKYKKRR"
     gene            complement(6776..7093)
                     /locus_tag="ACGI59_002548"
     CDS             complement(6776..7093)
                     /locus_tag="ACGI59_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9740330.1"
                     /translation="MDFGNINLILIGIIVIIGTTIIYLIKPKTAFCSKKYFNKLESIY
                     GNIDKKKTVKLEVLYRYVTGLEYIAIGLFTRRLDITIIAIILVAIITTALYYLIRKKY
                     ITI"
     gene            complement(7376..7672)
                     /locus_tag="ACGI59_002549"
     CDS             complement(7376..7672)
                     /locus_tag="ACGI59_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9740331.1"
                     /translation="MYFKTYILKREVNNKQLEITRSFDLDNLICTATEILKDNEIDLI
                     ITDEVNNVVWRNEKSTPPARECLVIAIEVYWWYTSIFLLYHINYVIKKNYLNRF"
     gene            complement(7691..8104)
                     /locus_tag="ACGI59_002550"
     CDS             complement(7691..8104)
                     /locus_tag="ACGI59_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9740332.1"
                     /translation="MKVKSKRSFIVGIIVCVLCCASLVIYCILKDKRFLISSFLLIVI
                     AIFNFCNAFSRKGIVEELHDSTDERDLYLTMKTSHILVKIMNYTLFTFTFLFIIAYSA
                     WKNQSLLVIAITLCVIEIFLFVAYLLINIFLEKKE"
     gene            complement(8088..8321)
                     /locus_tag="ACGI59_002551"
     CDS             complement(8088..8321)
                     /locus_tag="ACGI59_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFG9740333.1"
                     /translation="MKTKVREFRTNMGLTQQQLADLVHVSNRTIISIEKEQYSPSLML
                     AYRMAQVFGTTIEELCCLKENKEMEDKKHESKE"
     gene            8540..8914
                     /locus_tag="ACGI59_002552"
     CDS             8540..8914
                     /locus_tag="ACGI59_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HFG9740334.1"
                     /translation="MKISKLPEAELKVMRYICEANKVLTSRELVAAMEEKYEWNESTT
                     FTVLKRLERREFLSTEKIGKLTHYNILVKEKKYLRFETKEFLKNIHKNSISSLMSALH
                     GEDDEVDEDKLSELEECFKNLK"
     gene            9526..10218
                     /locus_tag="ACGI59_002553"
     CDS             9526..10218
                     /locus_tag="ACGI59_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFG9740335.1"
                     /translation="MYNILVVDDDKEIVDSIEIYLKSEGFKIYKAYDGMKTLEIITEN
                     DIHLILMDIMMPKLDGIKATVKIREERNIPIILISAKSEDTDKIIGLNIGADDYITKP
                     FNLLELIARVKSNLRRYVTLGNYEDTCKEILKSGALELNTLTKEVKIDGENIKMTPIE
                     YKIIELLLENKGRVFSIDEIYEKVWNEESFNSENTVAVHIRRIREKIEINPKEPRFLK
                     VVWGVGYKIEKF"
     gene            10414..12474
                     /locus_tag="ACGI59_002554"
     CDS             10414..12474
                     /locus_tag="ACGI59_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HFG9740336.1"
                     /translation="MEKYRVLKLVSLIIISISFPLTILLVTIGLAEPKYNIQKTSTKS
                     GIFDISDDFYDSNIFDEGTMASLLPKFKLSVGGKLTSKEKGKFQQEGKSIDLSSSDSE
                     YDKIMINEQASEEYLSYLKNIKVYVINTYTGVFYTNTSYASLYDFKQKTKSYCNVEII
                     NKIGKSSYIKTINGEKFELNNLSLELNEVDEGFEAYVSFPEEPTIEDGIVYTNFQIFK
                     QATEKVRLFFVASLSLAIICLLSIILYIKIKGEVLKRNNVFLLIYSKIPMEFNVILVS
                     ISIAILTIYALAVSYHIDVIALVSLSLLIVSNNIFFINVQLSYLENKMCIFKTSIIVR
                     VILWLIKTVREVSNAIKKVSLARKVIVIVIISLALIMAISTRSIGMGLLFSICIFLGF
                     TIYITKRLSYLSYVIEGTERIKKGELDYKIKITGNDNFTSLAENINNIGEGLDRAIEE
                     QVKSERMKSDLITNVSHDLKTPLTSIINYVDLIKKEESIQPEYINDYINVLESKSKRL
                     KLLIEDLFEASRVSSGNIELQISKIDLVQLLRQSIGELEEKLSKNNLYLKLNVPNDKV
                     YIWADGRRMYRVFENLLSNIAKYSLEETRVYIDVYDYGENVKVTMKNISSYELNFDPS
                     EIMERFKRADESRNTEGSGLGLAIARDLVALQGGKFYIEIDGDLFKANLEFQKYEEEI
                     TNME"
     gene            complement(12679..13002)
                     /locus_tag="ACGI59_002555"
     CDS             complement(12679..13002)
                     /locus_tag="ACGI59_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFG9740337.1"
                     /translation="MNNNYIFFIIIGMYIVTYLPRVLPMLIFSKKEMPDFLKKIMKFI
                     PVAILTSLTAKDVFFNGDSLYLSIANPKIISFMIVLLVAYKFKSIGISIITGVISIYL
                     FGIIL"
     gene            complement(12995..13696)
                     /locus_tag="ACGI59_002556"
     CDS             complement(12995..13696)
                     /locus_tag="ACGI59_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFG9740338.1"
                     /translation="MERTQQLKESFIASIPIFLGYIPIGIVGGILLQKSGLSPFQIAL
                     MVTLVFGGSSQFIAASMISTGASVTSIVLTTFIVNLRHFLMSSNLNMYIKNKSPKFIL
                     PFCHTITDETFAVNYEKFTAGNWSDRNAIYLNFFCLISSVLANFIGAFLGESISIDSS
                     ISGFILTSMFIALIVSQIKNKIYIVVFISSIIISVILYALFKSNLVIIVASILASLIG
                     FFIEESYCKKEESYE"
     gene            complement(13974..15359)
                     /locus_tag="ACGI59_002557"
     CDS             complement(13974..15359)
                     /locus_tag="ACGI59_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFG9740339.1"
                     /translation="MPINSFENYPMNWKPKRPSKGQILYKALAEQLEQDINNGFLLPG
                     TKLPPQRELADFLDVNVSTISRAFKICEKKGLISGVTGSGTFVSYDTRSNLFLMSSNN
                     KITFIEMGTMNPDFTLEEMNTLFKHIIKEIDFKTIFQYGQRDGAKWQKEAIAKLIYKA
                     GLETTSDSLLPASGGQNAIVAILAGLFQHGDRIGVDPLTYPGIKTAAKMLGIQLIPIK
                     QEDNEISEEGLLYACKNENIKGLYIIPDYQNPTTHIMSQNGRKMIANIASKYNLIVIE
                     DAIHSLLNETHLNPVASYLPNQTIYITSLSKIIAPSLRLAYISTPKQYREALSSALYN
                     INLSQSYFLTEIAYRMITSGEADKLINARRKSARRRNKIINQYLSGYNLLGNEECIFR
                     WLILPDGIMAEKFEIQALKEGVQVYASERFAVGKEKPISAIRIAVCATESTEELKAGL
                     SVLKRLLEEKK"
     gene            complement(15467..16705)
                     /locus_tag="ACGI59_002558"
     CDS             complement(15467..16705)
                     /locus_tag="ACGI59_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HFG9740340.1"
                     /translation="MELFIEVIKFNIYISPLILFFILFTKKISKYTFKFNYFICLLIV
                     VRMMFISKINLNILNINNTTINIYKHNIDNYIPYDQGITLDIFMYIWILGIIAISFYY
                     LRTNFKFYNYIENFKKEILEIDITSILDEQAKELSINKKLQIYKVKGIYSPAIIGLTR
                     CKIIIPEKKYNNKELRFIFKHELIHYKRKDNLFKLLISIICIIYWFNPILYLLKKYFH
                     ELCELSCDEIVIDKCTTSEIKEYAYIILDTIKYQKSLKYSSFVSQFLNKKNIILKKRL
                     DSMFNQDKKKINLGLKFLLTFIIIGSIFSINSNFKQNEDVEYTIGKINYNKNGETYGT
                     HIIDSNGNSIEPELIQTIGRGKKVGYVKKRDLYDCDNQPKTPEEAVLYTRKRNFNIFK
                     KTIPLYDKEGIKIIGRFEIN"
     gene            complement(16718..17095)
                     /locus_tag="ACGI59_002559"
     CDS             complement(16718..17095)
                     /locus_tag="ACGI59_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HFG9740341.1"
                     /translation="MIILKKIPKSELLVMNFIWDKDIDKIPAREVANYMEISHGWTKG
                     TTGKVLSRLSDKGFLEFEKDGRNTLYTIKVKHEEYLKFETKDFLELIHKNSIHSFISS
                     LGCGNEISDDDIKELEQWLKKRK"
     gene            complement(17614..18036)
                     /locus_tag="ACGI59_002560"
     CDS             complement(17614..18036)
                     /locus_tag="ACGI59_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HFG9740342.1"
                     /translation="MNTFKTEVLLKYSAKDIFNIFTKSARLNFPNFNEKKAVGTFVQQ
                     KNNKRFKVEITNFEKNRVYEIKTSNKRESTITRYELVHIDLENTKLIFIESESKRNIF
                     GKINSALVRVLFGKRQPEEFNKFIKKLEMELENNSVNL"
BASE COUNT         7096 a         1996 c         2638 g         6388 t
ORIGIN      
        1 ttatataaat tttaatagaa tatgtatagt atatagtaga tagaatgtat aaaaataaaa
       61 actgttcaat aagaattaat atacattaat tcagctgaat ccaatcaaaa tacttaaata
      121 aaagtaatct gaaaataaca taattcaaat caatttattc aaataaacct caatttatct
      181 caaatcttaa gaagtcatta aacaaacttg ctaaattaca aagagaatta tcaagaaaaa
      241 caatcggtag tttaaataga aataaagcaa gattaaaagt tgcaagactt caagaacata
      301 tagctaatca aagaaatgat tttctacaaa aactatctac taaattaata aaagagaacg
      361 atattatttg catagaagat ttacaagtaa agaatatgat taggaatcgt aaactatctc
      421 gctcaatttc tgatgtgtca tggtcagagt ttattcgtca attagaatac aaggctaatt
      481 ggcatggtag acaaattgta aaggtgggta aattctttgc aagttcacaa atatgcaata
      541 aatgtgggta taaaaacgag gaagtgaagg acttaaatat aagagaatgg atttatccta
      601 gttgtaatga aactcacgat agagatataa atgcaagtat aaatatatta aaagaaggat
      661 taagactaat aacaattcaa aataaataac gtatgtaaga accgtaggaa ctacggggat
      721 agcctgtgga gaattagtaa gacatatgtt agtatgcaaa attctgtgaa gcaggaaacc
      781 tgtgacttta gtcatgggag gttcaggcta gtattactca ttattgtatt caaaagctaa
      841 agtggaaacc tagtgaatat atgaatttag aagttaatga gagagcatta gcagccgact
      901 caatacttat taaaattgaa gatgaagaag aaacaatgaa agaagctgaa agagagaaaa
      961 agagaggtag aagaaggtag aatttaaagt taaaatagtt acattgctaa tatatgaaaa
     1021 tgttacaaat ttggaaatag atggatatat atgttgtata gtataatgta gataatatat
     1081 caccaagtaa aaaacaacaa ataaatctaa attatgaggg gtggaaaata aagatattaa
     1141 acttacaata tgtatatttg cttttgtaat tggaataata gtgttatttt gctgtatagg
     1201 tctaggtcaa tttaatatgg attgtattat aaaacaaaat ggtgggtcaa tagctaccga
     1261 acaatacaac atatatttag aacaatctat ttcacaatat agaaattttg gttctatact
     1321 ggttttacta ggaggaatag gaatactgtt tgataaaagt gtcagaaaat actaaaaaag
     1381 tatattttta tttaggatgt gatgaatgat atggcaagag atgtatttag tgtaaagatg
     1441 acaccactag aagcacagaa aatggtagaa aaaaatttgg aagcagattt agtttctgtt
     1501 gatatttata acctagaaaa taataagtct ataatagtta caatctttga aaaatattat
     1561 gcaagaagca atagtgatgc aggtttggta gtgatttgtg aaaatactac aggaaaaaca
     1621 cttgttaaaa ttacctcaac tggtagtgca attggactat ttcagattga ttggggaaca
     1681 ggtaataatt tgattaaaag agtaaaaaaa atacttattg attatattat ttaattattt
     1741 tatgtagata ttcttaggtt ggtatggaat ttgtactaac ctattaagta atcaataaaa
     1801 ctaaataaac atagaaagca cttacaaata tgtaggtgct ttttattgaa aaaatagaaa
     1861 ataaaataaa aagtaagata taggaaaaat atgtaagaat tatatgttat aataattgta
     1921 gcaagaagat gtaatctaca atttatagag tggagttcat gtattaaaaa attatcctcc
     1981 caacgataag aagggaggtg aatatacatg gataattttt tatttaatgt gttagctagt
     2041 gtaacagcta gttacatagt ttatttgatt agcaagttaa tcaaaaaagt aaaaagccac
     2101 tctaccgcaa agagtgactt aaagattgat ataaatttta aatatcatcg taaaaaataa
     2161 ctttcagtta tagaacttca ctctaccgca aatagattgt agttcttctt gtttttatta
     2221 taccacaaat tagaaaaaat attgcatata atatttttta tagtcaataa aaatatgaaa
     2281 ttttttatac acaatgatga tgaatctaac tagatgaata aatttaattg taaataagaa
     2341 agtacttact taaatggtag gtgcttttgt tttgccaaat tatgctataa tattgagtga
     2401 gatgagtaat ttgtggggga attttataat gtggatatta gtaccaataa taacaatagt
     2461 attacttata atagctgttt cttctatgca atatatttta gttatgatag cttttttgtt
     2521 gattatatat tcttttatag aaaagaaaat agttatgggt cttgtgtcag ttcttttttt
     2581 cacttattca atttatcttt gtgcaacttg ggaagataaa tccttgatag cagataataa
     2641 agttgagact gttagggcac aaagagaaac tgtagaaaga gaaaaagaaa tggaaagaag
     2701 aagaatacaa gaagaagtag acaaagaaag atatattgaa aaacatggaa tggaaatatc
     2761 agaaaatgat ttaaaagtta agctagaggc tttagtacct caagaatata aggggaagaa
     2821 atatgaatta aaagtaggaa aatttaaaag atatagtatg tattttgatt taactgtaca
     2881 aaatgaaaaa tttagtaatt ctgaagaatg taaaaaattt gtaaaagaaa ttgctaatga
     2941 tcttaaaaaa attaaaataa gcaaggcgta ctttaagttt cattcaaaag atgacggcgg
     3001 tatatataat tctgtttata ttgattattt tagaaacata caaaataatg tagataatgt
     3061 tgaaaattta gaattcaatg agtttgaact taaaaccgaa gaagaagaga agcgagaaca
     3121 agagaaaata gaacaagaaa aaaacagtta taataactac attcaaaaca gagtggttga
     3181 cccactagat agaataaaga aactaaaaga gttgctagat tcaggagcaa ttacacagga
     3241 agaatataat aaaaagaaaa aagaattatt agaatagata atatagtaag cacttacaag
     3301 tatgtaggtg cttttaaatt tacaaagtat tccattttaa ttttatagtt tagattttat
     3361 gatataataa aaatatagaa gttttgcagt gtgcgatatt tgttacaaag tagggcttaa
     3421 tacttgaaat ctaagatgtt gagggtgcgt gataagtgtt atcaattgca ctattgcccg
     3481 ctcactgcaa ttttaagagt attgtatata tgtaggtatt ggaaatgcta agtttatttt
     3541 ggggttttag attaactata tggaatgtaa atattacatg ggtaaaaatt ttgatgattt
     3601 ttatgacagt tttagattaa ctatatggaa tgtaaattgt atagaattaa gaattttttt
     3661 aatactaatt atagttttag attaactata tggaatgtaa atattacatt taatttgcat
     3721 tgctttgatg acttctttgt tttagattaa ctatatggaa tgtaaatgcg tacaaggttg
     3781 agaagctccg caatctcaac gtcgtgtttt agattaacta tatggaatgt aaatatttga
     3841 aaggaataaa ttttaaatga atgaaaggat aggttttaga ttaactatat ggaatgtaaa
     3901 tcagccaaaa acttcgaatt tgaactaaaa tgcgtgggtt ttagattaac tatatggaat
     3961 gtaaattctc atgactattg aataaatata ataaacaagc atacgtttta gattaactat
     4021 atggaatgta aatttagaat caaaagcact taatcctagt tctttcatag ttttagatta
     4081 actatatgga atgtaaatag ctttttagct tcgtctacta ctttataagc aaagcgtttt
     4141 agattaacta tatggaatgt aaatgatgaa caataaaaca atcatctaaa gacgaagaaa
     4201 gttttagatt aactatatgg aatgtaaatg ttcctgttgt gttttttact aatcccattt
     4261 tatatgtttt agattaacta tatggaatgt aaattgctgt gatttcacta ataaaacaat
     4321 taataattgt gttttagatt aactatgtgg attcaaaatt aaataaacaa agaaagcact
     4381 tacaaatatg taggtgcttt tattctgctc aaaattggtc ggttgggtaa aatcattaga
     4441 aaaaattagt aaaaacctct tttctgtaac tcgttacaat attattatta atgtaacgag
     4501 ttacagaaaa gaggtgaata aaatagcaac taaaagtagg gcagagtata tgaaaaattg
     4561 tcgaaaagat aaaagaggtt ttagtgtact tttagacaaa gaaaagttag ataaatttga
     4621 tgaagtatta gaggaaaaga atctaaccaa gaaagaatgg ctagaagaaa aaatcgacga
     4681 ggaactggaa caaaaggaat aaaaaataag ggtcactccc accgaccaaa gtttgagtaa
     4741 cccctatgat gtatactatc gtatatcaat tataatatat gtcattcctt aaaaaaatca
     4801 attattaagg agtgtaatat tatgaaaaat gaattaatga tgtttgaagg aaaagagatt
     4861 gaagtatttg aatttgaagg aagaatttta ttcaactcaa aagatgtagc aaattgttta
     4921 gacataaaaa atgttaatga gaatataata cttatgaatg aaaagcaagt tgtaaagtta
     4981 agaaattcgg atatcagtaa tactgacatc cgaaaactta ataatgctgg agaaaatttt
     5041 cttactgaaa gtggagtgta taaattaatc tttaaatcaa gaaaagaaga agctgagaga
     5101 ttccaagact ggataagcga tgaagtttta ccaagcataa gacaaactgg tgcatacata
     5161 actaataatg taatcccgaa aagttgagag aaaaagcaag tgagattgaa aaattacagt
     5221 tgcttataac agtacatcta tgttaaaaga attgctagat gatgcaggct ttgacaataa
     5281 atcaaaacta ttaacagcta aaacattata taagaaagca gaaattgatt taccaataga
     5341 gataaacgaa gaagaacatt attttgatac aaaacaaata gcatctaaac tgaaaatata
     5401 ttctaagagt aataaaccag ctcagatggc tgtttgtgag attattaaaa agattgattt
     5461 agaagatggt gaagttaaag gtgtttggga aattaatggt tcttggactg gtactgtaaa
     5521 taaatataca aagagtgtaa tagataaggt gagaacttgg atagaggaaa ataatagacc
     5581 taccaagatt gcaggtgaga agaagaatta ttatgtattt tacaaaattg agtaaattta
     5641 tcagttgtat taaataattt agtttagttt tggggggatt aatacaatgt gtgagaattt
     5701 acttgataat atgtatattg aaaagagaaa agaagaatat agaattaaac ttttgaaaat
     5761 aagagaaaca gatatagata tatataataa gttagaaagt atagtatata aactttctga
     5821 gaaaaaatta gagaaaaata ataaatagac atataaagca cttggatatt ctactgtttc
     5881 aagtgcttgt ttggtataaa atggtataat ggagatagag aattatatta accaaaaaat
     5941 ggagaaaata gagaagatga tttagttgac gctttatcag taggagtacc tgcaggtaag
     6001 acagaatctc tagtacgatt agttggagat ggaataagtg atactcaaaa aagtttaata
     6061 aataaaagag aatttagaaa tataactcaa attggtggaa atggaaatga gaaagcattt
     6121 agtgaagtag aaaacttagt taaatagatt agaaggtagc aactagagtt ggttgttacc
     6181 ttcttttttt atactttctc tctctaagta ttttattttt catatcttct aatacatcta
     6241 atgcagtagt ataaaattgt atattatttc tataaggtat agctattgga agaatgccct
     6301 tgttaactaa aaaaataaga agtacaataa ataaaagtgt cataatcatt ctaataaaat
     6361 aattagtgat atctgataaa tcaaatgtta agttaattgt tgtagaaaat acaacagcca
     6421 taatagatat atacaaagca aaaatggggt tactattttt ttgtaactgt cctttaagcc
     6481 tagattgctc tatttctata tcttgaatac tttttatgga atccatttca ctataatact
     6541 tataaacttt taaataatat tttttatact tgtttaaagc ttcttcatta tctttaaggt
     6601 ctttgtcact taactctgat ttaacttccc caaaatccat aaaatccctc ctacaatgtt
     6661 atataatata ctttaattta tagcatattt aagaacttat atcagcaatt tgtcgaacga
     6721 ttttagctaa aaatagagta taatgatagg cttattattt ataagatatt attttttata
     6781 tagttatata tttctttcta attagataat ataatgctgt tgttataatg gctactaata
     6841 ttattgctat tattgtaata tctaatcttc ttgtaaataa gcctattgct atatactcaa
     6901 gacctgttac atagcgataa agtacttcta gtttaacagt ttttttctta tctatatttc
     6961 catatattga ttctaattta ttaaaatatt ttttactaca aaaagcagtt ttaggtttaa
     7021 ttaaatatat tattgtagtt cctattataa ctattattcc aataagtata agattgatat
     7081 ttccaaaatc catagcagat acctcctatt aaatttgctt ttcttaatca aagtatatca
     7141 gtaacacaag tacaaagagt atctaaaata ttacaaatat taacaggata gataaaacta
     7201 tagtaaaaat tgtaataata aagcgaacga aaattatcat actaaaacat aagacatgtt
     7261 ataattgtat tagatagaag attaacatac accaaagcac tccttataat aaagagtgct
     7321 tatttttttg aaattcatca atatataaac tatcaagaac cttattcaac acaccttaaa
     7381 atcgatttaa atagttcttt ttgataacat aatttatatg atataataaa aaaatagaag
     7441 tgtaccacca atacacttct atagctataa ctaagcattc tcttgctggg ggagtgcttt
     7501 tttcatttct ccaaacaaca ttatttactt catcagtaat tatcaaatca atctcattat
     7561 ctttcaaaat ctctgttgca gtacaaatta gattatctaa gtcaaaactt cgagttatct
     7621 ctagttgttt gttatttact tctcttttta gtatataagt tttaaaatac atttatatca
     7681 ccccttgtat ttactctttt ttctctaaaa atatattgat aagtaaatat gcaacaaata
     7741 aaaaaatctc aatgacacat agtgttatag ctataactag aagtgattga tttttccatg
     7801 cactataagc tatgatgaaa agaaatgtaa aagtaaataa tgtataattc ataattttaa
     7861 ctaagatatg acttgttttc attgttaagt acaagtctct ttcatctgta ctatcgtgta
     7921 attcttcaac aatgcctttt cttgaaaaag cattacaaaa attaaagatt gctattacaa
     7981 ttaaaagaaa tgatgaaatt aggaatcttt tatcttttaa aatgcaataa ataactaaac
     8041 tagcacaaca taacacacaa actataatac ccactataaa acttcgttta ctctttactt
     8101 tcatgttttt tatcctccat ttctttattt tcttttaaac aacatagttc ttcaatcgtt
     8161 gtaccaaaca cttgtgccat acgataggca agcataagag aagggctata ttgctctttt
     8221 tcaatagaaa taattgttct gtttgataca tggactaaat cagcgagttg ttgctgtgtt
     8281 agacccatat ttgttcgaaa ttctcgtact tttgttttca ttccataacc tctttctata
     8341 tgaagctaac ttcatgtgaa gttaacttca taatatccta aaatgagtaa aatgtcaatc
     8401 tcattagata agtattataa aacctaaaag aataatatgg taaaaattgg atggaattat
     8461 ttaccttaat tctcttgttt gtaaaacttt attgatatag aatttaacat atacataaat
     8521 cggcaaaggg gcgcgaatta tgaaaattag taaattacca gaagcggaat taaaagttat
     8581 gagatatatt tgtgaagcta acaaggtatt aacttctagg gaattagttg ctgcaatgga
     8641 agaaaaatat gagtggaatg aatcaactac atttacagtt ttgaaaagat tagagcgaag
     8701 agagttttta agtacagaga aaataggcaa acttacacat tataatatac tagtaaaaga
     8761 gaaaaaatat ctaaggtttg aaacaaagga atttttgaag aacatacata aaaattcaat
     8821 ttcaagttta atgtcagcgt tacatggtga agacgacgaa gtggatgaag ataaattaag
     8881 tgaactcgaa gaatgtttta agaatttgaa ataagaaaat gacaaattga tacataataa
     8941 ataaaatgaa ggtatgaaat tataactttt tctaccttca ttttgtttat tattctgaac
     9001 atgttaagaa aatgaaataa gtttgtattt tagttatata aattttaata gaatatgtac
     9061 agtatatagt agatagaatg tataaaaata gaatttaaat aagtagctat tattgcagtg
     9121 aaatgaatgt ttctaagtat atttgggtgg aagaatggtg gaagaaaatt agtgaaatgt
     9181 aattcttcca ccattcttcc acctagaaaa tagaaactgt ttaataagaa ttaatataca
     9241 ttaattcagc tgaatgcaat caaaatactt aaatgaaagt aatctgaaaa caacataatt
     9301 caaatcaatt tattcaaata aacctcaatt tatcttaaat cttaagaaat atttaagaat
     9361 taggataata ttttcttaag atttatattt tattatatag ttataaggga aagttacaaa
     9421 aatgtaagcc aaagtaatat aacaatttag tcattgttga gaaagggagt gaaagatatt
     9481 gtataatata gataagtatt attatacaat agaggaagtg aaaatatgta caatatatta
     9541 gtagtagacg atgataaaga aattgttgat tcgatagaaa tatatcttaa atcagaagga
     9601 tttaaaatat ataaagcata tgatggaatg aaaactttgg aaataataac agagaatgac
     9661 attcatctaa tactaatgga tataatgatg cctaaattag atggaatcaa agcaactgta
     9721 aaaatacgag aagaaagaaa tattccaata attttaattt cagcaaaaag tgaagataca
     9781 gataaaataa taggattaaa tattggagct gatgattata taacaaagcc atttaatcta
     9841 ttagagctta tagctagggt taaatcaaac ttaagacgat atgtaacttt aggtaattat
     9901 gaagatacat gtaaagaaat tctaaaaagt ggagctttgg agttaaatac actaacaaaa
     9961 gaagttaaaa ttgatggtga aaacataaaa atgactccaa ttgagtataa aataattgaa
    10021 cttcttttag aaaataaagg acgagttttc tcaatagatg agatatacga aaaagtatgg
    10081 aatgaggaaa gttttaattc agaaaataca gtagcagtac atatcagacg aataagagaa
    10141 aagattgaaa taaatccaaa agaaccaaga tttttaaagg tggtgtgggg agttggttat
    10201 aagattgaaa aattttagtg gaaaaattat aaaagaaaag agtttaatat ttaattgcat
    10261 tttgatttta gtcattttta catttacaca aatgttagga attttaatat gttttaattt
    10321 tttctctgag agtttaattt actctatggg gattatgctt ttcttaaact ttattttata
    10381 taaaatactg acaatgtaga tgaggtgggc aacttggaga aatatagagt attaaagtta
    10441 gtttcattaa tcataatatc tatctcattt ccattaacta tattgttggt gacaatagga
    10501 cttgctgagc ctaaatacaa tatacaaaaa acttctacaa agagtgggat ttttgatata
    10561 tctgatgatt tttatgatag taatatattt gacgaaggaa caatggcaag tctattaccc
    10621 aagtttaagc tatctgtagg tggtaaatta actagtaaag aaaaaggtaa attccaacaa
    10681 gaaggtaaaa gtatagattt aagtagtagt gattctgagt atgataaaat catgattaat
    10741 gaacaagctt ctgaagaata tttaagttat ctaaaaaata taaaagtgta tgttattaat
    10801 acatacacag gagtatttta tacaaatacg tcatatgcta gtttatatga ttttaaacaa
    10861 aaaactaaga gctattgtaa tgtagagata ataaataaga taggaaagag ctcatatatt
    10921 aaaacaataa atggagaaaa atttgaactg aataatctga gtctggaact aaatgaagta
    10981 gatgaaggtt ttgaggcata tgtatctttt ccagaagagc caactataga agatggaata
    11041 gtatacacta attttcaaat atttaaacaa gctacagaaa aagtaagatt attttttgta
    11101 gctagtttaa gtttagcaat tatatgttta ttatctataa ttttatatat caaaataaaa
    11161 ggtgaagttt taaaaagaaa taatgtattt ttactaatat acagtaagat acctatggaa
    11221 tttaatgtaa tattagtatc tatttcaatt gcaattttaa ctatatatgc tcttgctgta
    11281 tcttatcata ttgatgtaat tgcattagtt tctttatctt tactgatagt atcaaataat
    11341 atatttttta taaatgtgca attgagctat ttagaaaata aaatgtgtat atttaagaca
    11401 agtattatag taagagtgat tttatggtta ataaaaactg taagggaggt cagtaacgct
    11461 ataaagaaag tatctttagc aaggaaagtt attgttatag tgataatatc tttagcttta
    11521 atcatggcaa ttagcactcg aagtataggt atgggattac tgttttctat atgtattttt
    11581 ttaggattta caatatatat aactaaaaga ctttcatatc ttagttacgt aatagaaggt
    11641 acagaaagaa taaaaaaggg agaattagat tataagataa aaattactgg gaatgataat
    11701 tttacgtctt tagctgaaaa tataaataat attggagaag gtcttgatag agctatagaa
    11761 gagcaagtaa aaagtgaaag aatgaagtct gaccttataa caaatgtatc tcatgatttg
    11821 aaaactccac ttacttctat aataaattat gtagatttaa ttaaaaaaga agaatcaata
    11881 caaccagagt atataaatga ttatataaat gtacttgagt caaagtcaaa aagactaaaa
    11941 ttattgatag aagatttatt tgaagcgagt agagtaagta gtggcaatat agaattacaa
    12001 attagcaaga ttgatttagt gcaactatta agacaaagta taggtgaact tgaagaaaaa
    12061 ttatctaaaa ataatttata tttaaaatta aatgttccaa atgataaggt gtatatatgg
    12121 gcagatggaa gaagaatgta tagagtattt gaaaatcttt tatctaatat agcaaaatat
    12181 tccttagaag aaactagagt ctatattgat gtatatgact atggtgaaaa tgtaaaggta
    12241 actatgaaaa atatatcttc ttatgaatta aattttgacc caagtgagat tatggaaaga
    12301 tttaaaagag ctgatgaatc aaggaatact gaaggaagtg gattagggct tgctattgca
    12361 agagatttag tagctttaca aggtggaaaa ttttatatag aaatagatgg agatttattt
    12421 aaagcaaatt tagagtttca aaaatatgaa gaagaaataa caaatatgga atgagatagt
    12481 atttaagtat atatattttt ttactgtcga aagcttttat tataatatat tagaatattt
    12541 acagtagaga atgaagagta aaaacagagt aactttaagt cactctgttt tttatttaaa
    12601 atcaataata taacaactta aattaaaatt gtacacatag ttttaaaata acttatacaa
    12661 taaaacatta tccaaatatc acaagataat tccaaataaa tatatagata tcactcctgt
    12721 aataatagaa ataccaatag atttaaactt atatgcaaca agaagtacaa tcataaaaga
    12781 tataatttta gggttagcta tagataaata tagactatct ccattaaaaa atacatcttt
    12841 agcagttaag gaagtaagta ttgctacagg tataaatttc atgatttttt tcaaaaaatc
    12901 aggcatctct ttttttgaaa aaattaacat aggaagcact cttggtagat atgtaacaat
    12961 gtacattcca attataataa aaaatatata attattattc ataagattcc tcctttttac
    13021 agtaagattc ttctataaag aaaccaatta aagatgctaa aattgatgca actattatta
    13081 ctaaattgct tttgaataat gcatataaaa taacagaaat aatgattgat gatataaata
    13141 ctacaatata aatcttattt tttatttgag aaactattaa agctataaac atagaagtca
    13201 atatgaagcc agaaatagaa ctgtctatag atatactttc tcctaaaaat gctccaataa
    13261 aattagctaa aactgatgaa attaagcaga agaaattaag ataaatagca tttctatcag
    13321 accaatttcc agcagtaaat ttttcataat taactgcaaa agtttcatct gttatagtat
    13381 gacaaaatgg caatataaat tttggacttt tattttttat gtacatgttc aaattagaac
    13441 tcattagaaa atgtcttaag ttaactataa atgtagtcaa aacaatagat gttacacttg
    13501 caccagtaga aatcattgaa gctgcaatga attgagaact tcctccaaat actaaagtta
    13561 ccattagtgc tatttgaaaa ggtgaaagac cagatttttg taatagtatt cctcctacta
    13621 tacctatagg tatataacct aagaatatag gaattgaagc aataaagctt tcttttaatt
    13681 gttgagttct ttccacaata aatcactcct tttgtattaa atgtattaaa gtattcgtaa
    13741 aacagaaaaa tagattaatt gtataattgc aattataatt aaaaataaat ttggattaat
    13801 atataaaata ctatacaaca aatagataat cttaaacggt tattaaattt tatctatttg
    13861 ttattatgat gtaataatac atattaatct ctataagtta attactatct ggtgtaattt
    13921 tattttaaca acaaaaaaaa atacacacaa ttaaaatatt gtgtgcaata caattatttt
    13981 ttctcttcta atagtctttt taatacgctc aaaccagctt taagttcttc tgtactttct
    14041 gtagcacata cagctattct tattgcagaa attggttttt cttttccaac agcaaaacgt
    14101 tcagaagcat agacctgtac accttcttta agtgcttgta tctcaaattt ttctgccatt
    14161 attccatcag gtagaatcaa ccatctaaat atacattctt catttcccaa aagattatat
    14221 ccagataaat attgattaat aatcttgttt cttcttcttg cagatttacg gcgtgcatta
    14281 attaatttat cggcttcacc tgatgtaatc atgcgataag caatttctgt taaaaaataa
    14341 gattgagata gattaatgtt gtataatgcg cttgataatg cctctcgata ctgtttaggt
    14401 gtagagatat atgctagccg taaactaggt gcaataattt ttgataaact cgtaatataa
    14461 attgtttgat tgggcagata ggaagccact ggatttaaat gtgtttcatt aagtaaacta
    14521 tgaatagcat cctcaataac aattaaattg tatttagatg caatatttgc aatcattttt
    14581 cgtccatttt gtgacattat atgtgtagtt ggattttgat aatctggtat tatgtataga
    14641 cctttaatat tttcattttt acaagcatat aatagtcctt cttcactaat ctcattatct
    14701 tcttgtttaa ttggaattag ttgaatccca agcattttag ctgctgtctt tattccagga
    14761 taagttaatg ggtctacacc tattctatct ccatgttgaa acaaaccagc taaaatagca
    14821 acaattgcat tttgtccacc acttgcagga agtaagctat ctgatgtagt ttcaagccca
    14881 gctttataaa ttaatttggc aatagcttct ttttgccatt tagcaccatc tctttgtcca
    14941 tattggaata tggttttaaa atctatttcc ttaattatgt gtttaaacaa agtattcatt
    15001 tcttctagtg tgaaatctgg attcattgtg cccatttcaa taaatgtaat tttattatta
    15061 ctggacatca agaatagatt tgaacgtgta tcataagata caaaagttcc acttcctgta
    15121 actccactta ttagaccttt tttttcacaa attttaaaag ctctagagat agtactcaca
    15181 ttaacatcta agaaatctgc caattcacgt tggggtggta atttagtacc tggtaataaa
    15241 aatccattgt tgatatcttg ttctagttgt tctgcaagcg ccttatataa tatttgccct
    15301 tttgaaggtc tctttggttt ccagttcata ggataatttt caaaagaatt aattggcata
    15361 agttttcacc tcacattatt aatttgattt tttctttatc taaaacaata aattctaaag
    15421 atacaaataa aaactgatag gaaataattc tatcagtttt tgtcttttaa ttgatttcaa
    15481 atcttcctat aatttttata ccttctttat cataaagagg gattgttttt ttaaatatat
    15541 taaaatttct ttttcttgta tataaaacag cttcttctgg tgtctttggt tgattatcac
    15601 aatcgtacaa atctcttttt tttacgtagc cgactttttt acctctacct attgtttgta
    15661 taagttctgg ttcaatggaa tttccatttg agtctattat atgtgtacca taggtttctc
    15721 catttttatt ataattaatc ttaccaatag tatattctac atcttcattt tgtttaaaat
    15781 tggagttgat agaaaatatt gaccctatta ttataaaggt taataaaaac ttaagaccta
    15841 aattaatttt ctttttatct tgattaaaca tagaatctaa cctctttttt aaaataatat
    15901 tttttttatt tagaaactga gatacgaatg atgaatactt caaagatttt tgatacttta
    15961 ttgtatctaa tattatatat gcatactctt taatttcact cgtggtacac ttgtcaatca
    16021 caatttcatc acaagataat tcacataatt catgaaaata tttttttaat aagtataaaa
    16081 ttggattgaa ccaatatatg atacaaatta tactaattag aagcttaaat aagttatctt
    16141 ttcttttata atgaattaat tcatgtttaa agatgaatct taactcttta ttattgtact
    16201 ttttttctgg aattattatt ttacagcgtg ttaaacctat aatggcagga gaataaatac
    16261 ctttaacctt atatatctga agttttttat ttatacttag ttcttttgct tgttcatcta
    16321 agatactagt aatatctatt tctaaaattt cttttttaaa attttctatg taattataaa
    16381 atttaaaatt tgttcttaaa tagtaaaaag agattgctat aattcctaat atccagatgt
    16441 acataaatat atctaaagta atgccctggt catatggtat ataattatcg atattatgct
    16501 tatagatatt aatagtagta ttattgatat ttaatatatt taaatttatt ttagatataa
    16561 acatcattct gactactatt aaaagacata taaaataatt aaacttaaaa gtatatttgc
    16621 ttatcttttt agtaaataaa atgaaaaaaa gaattagtgg acttatgtaa atattaaatt
    16681 taataacttc tataaataat tccataaaat acgtctccta ttttcttttt tttagccatt
    16741 gttcaagttc ttttatgtca tcatctgata tttcatttcc acaacctaat gatgatataa
    16801 agctatgaat agaattttta tgtataagct ctaaaaaatc ctttgtttca aattttagat
    16861 attcctcatg tttaacttta atagtgtata aagtatttct accatctttt tcaaattcta
    16921 aaaaaccttt atcacttaat ctagaaagta ctttaccagt agtacctttt gtccatccat
    16981 gagaaatttc catataatta gctacttctc tggctggtat tttatctatg tctttatccc
    17041 atatgaaatt cataactaac aactcggatt ttggtatttt tttcaatata atcactcacc
    17101 tttaacttga ttatattaga caaatgtcta atagtcaata taatatatca tcatgtataa
    17161 ataatcttta tattatttta tgcttgagaa ctaagtagta tagatttata gataaaaaag
    17221 atattgagag ttttatggaa gaggggaata tattttctat tagagataag aatatgacat
    17281 atatttttta ttgattttta caaaaatact atatagtgaa taaattaata tcttttctaa
    17341 attagaagag gagaatctta ttagataaag attatcctct ttattgaaag tttaagggat
    17401 attagagatt attaattttt ctctcttttt ttaactaaaa gcaataatac ataaaattat
    17461 tattgctaat gatactctta atgtggtata tagtcatagt gtcacatgtt tatcaatagt
    17521 tttttcatta attattgtaa cgaatagtag gactcataaa atacttagaa atatgttttt
    17581 agatagattt taaatatata atctaactta tatctataaa ttaacactat tattctccaa
    17641 ttccatttct agttttttta taaatttatt aaattcttct ggttgccttt ttccaaacaa
    17701 tactcttaca agagctgaat tgatttttcc aaatatattc ctcttgctct cactttcaat
    17761 aaaaataagt tttgtatttt ctaagtcaat atgtactaat tcatatctag taatagtact
    17821 ttctctttta ttgcttgttt ttatctcata tactctattt ttttcgaaat tagttatttc
    17881 aaccttaaat cttttattat ttttttgctg tacaaatgtt cctactgctt ttttctcatt
    17941 aaaattagga aaatttagtc ttgcagattt tgtaaatata ttaaatatgt cttttgcaga
    18001 atattttaat aaaacttcag ttttaaatgt attcatatcg ttttcccttc tgtatataaa
    18061 tttaatattt ataagttaat ttttaagtta aaatagtata atatgccata ttaaatca
//
LOCUS       DAYAPA010000082        17991 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.082, whole genome shotgun sequence.
ACCESSION   DAYAPA010000082 DAYAPA010000000
VERSION     DAYAPA010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17991
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.082"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            405..3950
                     /locus_tag="ACGI59_002561"
     CDS             405..3950
                     /locus_tag="ACGI59_002561"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFG9740343.1"
                     /translation="MNRLKPRILGISILFIIIIILTCGAVFKFKFKLDNKLKETTHTY
                     LSEFTEHNIINLKTRLSGQFDMLESIANFIGNLNNINDEMVIDLMDSDVKRSSLIRMT
                     ISTLDGECYSNDGITHNVQDRDYFKKAKNGEKNISEPLKSIVNNKEIIVLSTPVYKSG
                     KIVGVLSGIYDSKKLNELLGLSAFDEKADVFVAKKNGDIVAQKTNKNRKTLSCIFQLF
                     DKDNCDEDDCLKTIKENMKNDKSGYFNYNLDNEKFMLNYQPLGVNDWYVFSVVPNKIV
                     SIQSENISYDVYIFALEILLVILIFIIYIACIINNNTKIIINEKEKFKVLTDNILGGV
                     KTCLKDEYMTMTYVSQGFLELTGYSCEDLNKIFKNRFKDMIYKDDLDKFNCIILNQLE
                     KQDKIEVEYRIVRKDGSIIWILERSKSINDNKGSECIQGVLTDITELKKIQEELDSKR
                     FEIQAINDSIVGGIVITEINDDFDSIYLNEGYLNMIGYTRKQLEDELNNKLIKLIFKD
                     DRTELINSINKQLKLSDTFASEQRVEKRDGTIIWILLKGRKMIYDNGKSVLCCVIVDI
                     TSAKLLEQELKKSEERHEIILKQTQNIIIEWDIEKDKMNISPNWYNKFKYDPITENAT
                     KNIIKSVDIYHDDMDKFISLIKLTSQGSEYEECEIRLRTRDTIYAWFSIKATTLFDNN
                     GKPYKVVGSLTDIDESKREAEKLKERAEKDPLTDLYNKKASQSLIEEYITLDKKRKGA
                     LMIIDIDDFKGINDNLGHLYGDAVLSEIANDLINLFRATDIVGRIGGDEFIVFIKDIS
                     ETRDIINKAEELVKTFERSFLGKGRNYKISLSVGIAKFPQDGMDYMTLFRNADIALYS
                     AKGKGKNCYTLYDKKFDKVQYITNTRNAGDSDNVEMSFKEHITNYIFDILYESSDIEV
                     AVNLILSIVGKHFNVSRMYIFENSDDNRVCKNTFEWCNEGVTSEINNLKNICYKDLGN
                     YLLNFNADGLFYCNDIENLGSDVKKVLKPQGIRSILQCKILEGNEFRGFIGFDECSYN
                     RMWTNDEVNTLTFISKTLSMFLLKMRIQSKLEESLTIQKSVMDNMDIWAYVVDRNTYE
                     LLFINKKMLESAPNTHVGDLCYKAIWNNQDKPCKKCPMIYLKDGDDTYKSFMHNKKFE
                     VQANITTTTLKWFDGKDACLIFGSEITSIEAEENT"
     gene            4135..6069
                     /locus_tag="ACGI59_002562"
     CDS             4135..6069
                     /locus_tag="ACGI59_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HFG9740344.1"
                     /translation="MENKFLPISKQDMIDRGWEELDFVLVTGDAYVDHHSFGTAIISR
                     VLESAGYKVGIIAQPDWRTTDDFMKLGKPRLAFLVNAGNMDSMVNHYSVSKKHREKDM
                     YSPGGKMGYRPDRATIVYCNKIREAYSDVAIVIGGIEASLRRFAHYDYWSDKVRKSML
                     IDSGADLLVYGMSEKQIVEVANALNDGYDPKYIRHIDGTCYISDTLEEIYDKYILIPS
                     YKEICEDKMKYVEAFKIQYDEQDPFRGNIIVQPHGSKYLVQNKPEKPLSREELDEVYG
                     LPYQKTYHPVYEKFGGIPAIEEVKFSIVSSRGCFGSCSFCAITFHQGRAVQSRSEKSI
                     IDEAIGITNLDDFKGYIHDVGGPTANFRRPACKKQITKGACKNRQCLSPSPCKNLDAD
                     HSEYLHLLRAVRKLPKIKKVFVRSGIRYDYVMADKNNKFLRELIEHHVSGQLKVAPEH
                     ISEEVLKYMQKPAGKTYDKFRQKFFAINEELGKKQYLIPYLMSSHPGSTLNSAIELAE
                     YLRDTHYQPEQVQDFYPTPGTLSTTMFYTGIDPLTMKPVYVPKSKRDKAMQRALLQYR
                     APRNYDLVYSALVEAGREDLIGFGHRCLIKPKNEKPYFNRNNSKKNVSKGTNKNKKTN
                     TNNRSNKNQQKSSTKKKKKH"
     gene            6175..7170
                     /locus_tag="ACGI59_002563"
     CDS             6175..7170
                     /locus_tag="ACGI59_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MqnA/MqnD/SBP family protein"
                     /protein_id="HFG9740345.1"
                     /translation="MKKYISIILLVVLTMVVLVGCSPGKDNPKNKELSVVKQVKVAVP
                     DGLPAVAIAKLANENPEIKEGYETVYSIEKTPEAISTRVMKKDADIAIVPSNMAAIAY
                     NKTSSYNIVGTVGMGSLYLVSTENIKDYSDLVGKEVGCTGKGLTPDITIKSLLLQKDI
                     NYSDIKFNYVNSASELVPLLATGKVKTGIVPEPALSALMSKNPDIKIIKSLNDSWKEV
                     SGSKDGYPQSTLIVKSEFLRDNKDFVDSFVGQLSNSIDWANKNPEELGAYSEKIKIST
                     ESKIIGKSLERANLKYIPVKDMIKDYKNYYEKLANFDDKTLGGKVPDEAIYFVEK"
     gene            7142..7927
                     /locus_tag="ACGI59_002564"
     CDS             7142..7927
                     /locus_tag="ACGI59_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9740346.1"
                     /translation="MKQYTLLRNKKNKEQFIDKMEVTLSCVILLFLWQIIALKIDNDI
                     FLPTVGQVFNSIKEIMLNSSFYIDILYSMGRCFFSFLLAMIFAIILSIVSYLNRFFRN
                     LLKPINALTRSIPTMILVVLALIWFEKDSTPFIVGFTIVFPILYDNVLGAILNIDKNL
                     LEMANVYKIRFIDKVLKIYLPAIKFQIISILVSTFSLSLKVAIAGEVYSQPTYGIGTM
                     IQTEKINFNTSGIFAWIIIVLLISAILQIAQKFLARRAFLWKR"
     gene            7915..8613
                     /locus_tag="ACGI59_002565"
     CDS             7915..8613
                     /locus_tag="ACGI59_002565"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9740347.1"
                     /translation="MEKIKLSIENINKRYDSRIIFRDFNIDFYVNEVNCILGKSGCGK
                     TTLLNIISGIIKNDTNNLNIKENLNRVGNKLEASYIFQDDRLIDWLTVEENIKIVVNK
                     YYNKTQLNKICDEYLELVGISDYKKFYPQMLSGGIRQRVNIARAFIYPSKNIIMDEPF
                     KSIDAKNTQLIMDNFRNILRKEKRTVLFVTHNIEEALFLADRIFILGDSPIRIKKILK
                     NSKELEKNEVLKLI"
     gene            8736..9209
                     /gene="ybaK"
                     /locus_tag="ACGI59_002566"
     CDS             8736..9209
                     /gene="ybaK"
                     /locus_tag="ACGI59_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HFG9740348.1"
                     /translation="MVKTNAMRILDSNKIDYKVMSYEVKSEHVDGVEVAHDIGRDVNE
                     VYKTLVTQGVSKNIYVYVIPVHENLDLKKAAKVAKEKSVEMIHVKDINKLTGYIRGGC
                     SPIGMKKFYKTFVNESAKNLDTIIVSAGKIGYQIELSPFDLQRLIKVEFVDVIKK"
     gene            9406..10245
                     /locus_tag="ACGI59_002567"
     CDS             9406..10245
                     /locus_tag="ACGI59_002567"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HFG9740349.1"
                     /translation="MRKIKSEQIVEQVKKLCIEASLYLGEDVLSCIKEKAKSEKSEVG
                     KNILNILVENAEIAKEKNIPICQDTGMAVFFVEIGQEVLIEGDTLTDAINEGVRQGYE
                     EGYLRKSVVSPINRVNTKDNTPAVIHYDMVKGDKIKIEFAAKGFGSENMSKMKMLKPS
                     DGLEGIKKFIIDTVSEAGPNPCPPMVIGVGIGGTVDKCAQIAKKALFRELGEFNKDEN
                     IAKLESELLTAINKLGIGPQGLGGTTTALGLNIETFPTHIAGLPVVVNINCHASRHKN
                     VVI"
     gene            10258..10806
                     /locus_tag="ACGI59_002568"
     CDS             10258..10806
                     /locus_tag="ACGI59_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S-containing hydro-lyase"
                     /protein_id="HFG9740350.1"
                     /translation="MIKITTPVNEIDIAKLNCGDTISLSGILYTARDAAHKRLIDCIN
                     KGEELPFDVYGQGIYYVGPTPTKPGEVIGAAGPTTSYRMDDLTIPLLERGLRLMIGKG
                     KRSDEVIEGMQKYGAVYLAAIGGAGAYISNSIKSCEIIAYEDLGAEAIRKIVVEDLKL
                     TVAIDSYGNNIYEQGRAIYECK"
     gene            10872..12053
                     /locus_tag="ACGI59_002569"
     CDS             10872..12053
                     /locus_tag="ACGI59_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HFG9740351.1"
                     /translation="MSKDYAKLALEMHEINKGKVSVESKVEIKTKDDLSTAYTPGVAE
                     PCLKIHENQDDVYRYTSKGNLVAVVSDGSAVLGLGNIGAEASIPVMEGKAILFKQFAD
                     VDAFPICLKTNDVDEIVKSVELMEPVFGGINLEDISSPRCFEIEERLKKSLSIPVFHD
                     DQHGTAIIVAAAIINSIKLIENKKIEDLEIVINGAGAAGIAIAKILLNMNVKNIILCD
                     RSGALEASIENLNYVQKEMLKVTNIRNEKGPLKDIIKGKDVFIGVSGPGAVTKEMVES
                     MSEKPIILAMANPTPEIMPEEAKLGGAFIMGTGRSDFPNQVNNVLAFPGIFRGALDVK
                     AKEINEEMKIAAAYAIANTISDEEICPEYILPDVFNKNVVKNVAKAVKEAAIKTKVNR
                     I"
     gene            12345..12734
                     /locus_tag="ACGI59_002570"
     CDS             12345..12734
                     /locus_tag="ACGI59_002570"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFG9740352.1"
                     /translation="MMEYKVNDQELNASMFIPFVNRIWPGDYDMDKTQTALSKTLNIT
                     AYDDKVLVGCLRVLSDGYYFGTITELLVLPEYQKQGIGSKLFQLAKENTPTMLYFGAQ
                     PGVEAFYEKNGCQRSLQSYIIKKKDSY"
     gene            12884..13147
                     /locus_tag="ACGI59_002571"
     CDS             12884..13147
                     /locus_tag="ACGI59_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFG9740353.1"
                     /translation="MEETKFCQCCAMPMGQTDELYGTNKDGSKSTDYCSYCFKDGEFT
                     ADISMEEMIEVCIPHMLQSNKDMTEDEARTMMNNFFPTLKRWK"
     gene            complement(13276..14118)
                     /locus_tag="ACGI59_002572"
     CDS             complement(13276..14118)
                     /locus_tag="ACGI59_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1-like domain-containing RNA-binding protein"
                     /protein_id="HFG9740354.1"
                     /translation="MINIGDFNKLTVKRKTEFGYFLDGQTNNTKDDILLHNRLIGKNE
                     INIGDEVNAFIFKDSDDRTAATLIPPLAKVGDVAHLKVVDNTDIGTFIDMGLPKDILV
                     PFKAKTYPLFRDEKYLFYIYLDKSKRIAATTDIDSYLLTDHTYNVGDIVTGVVYGFQT
                     NNSAMICVDNKYAGVILHNEYFTELKTGDVLENLHVIKIYEDGKLGLSPRGNRKDELD
                     TLENKILSYLEGSDGYMRFNDKSDPKDISILFNSSKKNFKRALGVLMKKGLIYQDEEG
                     TYLK"
     gene            14413..15147
                     /locus_tag="ACGI59_002573"
     CDS             14413..15147
                     /locus_tag="ACGI59_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFG9740355.1"
                     /translation="MKIVNKSSNIPLHTQLSSIIREMIETGELKEGDAIMPERELCNI
                     QNVSRMTVNKTIVGLVTEGLLYRVQGKGTFVAKQKKKYQFSNVKGFTDVMKEKGVNIK
                     TDILSFEMELPDDLVKRKLGISDNTTNIYKIVRLRYTDGEPFGLEIVYLSEEMCKGLT
                     KGILDNSSLYRVLNEKYGYKIQKAEQVMEPVILSDEESKLLETDEGALALKLHRNSYN
                     REGSPIEYTISIFRTDKYQYEIVLSE"
     gene            15157..16290
                     /gene="nagA"
                     /locus_tag="ACGI59_002574"
     CDS             15157..16290
                     /gene="nagA"
                     /locus_tag="ACGI59_002574"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFG9740356.1"
                     /translation="MKCLINGKIILKNQILENKVLVFDEKIIDIADSVPKDCEVIDAD
                     GKYISPGLIDIHIHGNMGKDTMDSTDESIETISKSIMRHGVTSFLPTTMTMDKEHVYD
                     ALEVIKKAQNRKLEGAQVLGAHLEGPFINENYKGAQNEKFIINSKYEFIKEYKDVIKV
                     ITYAPEKDIDFDFTREIKRCTDIVLSIGHSNANYDQAKEAINLGVTNVTHMFNAMTGL
                     NHRDPGVVGAALTTNVYSELIADTIHINKDLFQFILNNKGKERLILITDSIEAGGLED
                     GNYSLGGQKVIVKGNEARLENGALAGSVLSLNKMVFNFLDNTNLKVNEAINLASLNPA
                     TSLGINDKKGSLEIGKDADIAVFDENLDCKMTLCLGDVVYKNI"
     gene            16389..17138
                     /gene="nagB"
                     /locus_tag="ACGI59_002575"
     CDS             16389..17138
                     /gene="nagB"
                     /locus_tag="ACGI59_002575"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFG9740357.1"
                     /translation="MRVLVCKDYDGMSKKAAEMIAAQIVLKPNSILGLATGSTPVGMY
                     RDLVKKYNDNIVDFSDVMSFNLDEYYKLPISNDQSYDYFMKENLFNHVNIKPENTHLP
                     NGMADDIEKECMNYEASIDAAGGIDVQVLGIGRNAHIGFNEPDTKFAKRTHVVELTES
                     TIEANARFFKSREDVPKKAVSMGIGSILKSKKILLLASGEEKADAVYNTVYGDITPEV
                     PGSILQLHKDTIVIVDEAAASKLNPKDYKLV"
     gene            17313..17585
                     /locus_tag="ACGI59_002576"
     CDS             17313..17585
                     /locus_tag="ACGI59_002576"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9740358.1"
                     /translation="MLKIIMFSGILSSIIKLPIIGILPTGNIKKNTGISDSKKDGKQR
                     RSNLGISIYQLPSFLYSLINNSVPSNKPIPPEEIKLIKMYLLKSCI"
BASE COUNT         7208 a         1890 c         3153 g         5740 t
ORIGIN      
        1 gtttttatat ggtttaaatt ttatgtatac aaaattaatt tgctaactga atcatataaa
       61 aaaaaacaaa atatttttta gagggaatga ttagatagaa tttttttgtt atgaaattaa
      121 ttgaagagta attaattaga aaaggaatga ataataatga atatattaca ttattacttt
      181 ttgataaatc aaaaataaag ttataatttt taatattaat taagaatgat gaaatatgaa
      241 taaagatttt acaaataaat cttaagctat cttatttaat tctaatacag gaagaaggta
      301 aatgtatatt gaaaaaatca tagaaataga agtaaacaat ttaaataaat acaaaggttt
      361 gcaaaagaag taatataatt atatacaatg aggatgtgag tatgatgaac agactaaagc
      421 caagaatttt aggtattagt atcttgttta ttattataat tattttaaca tgtggagctg
      481 ttttcaagtt taaatttaaa ttagataata aattaaaaga aaccacacat acatatttgt
      541 ctgagtttac agaacacaat ataattaatc tcaagacaag acttagtggt caatttgaca
      601 tgcttgaatc tattgctaac ttcataggaa acttgaataa tataaatgat gaaatggtga
      661 ttgatttaat ggattctgat gtaaagcgaa gttctttaat tagaatgact atctctacat
      721 tagatgggga atgttattct aatgatggaa ttacccataa tgttcaagat agagattatt
      781 ttaaaaaagc taaaaatgga gaaaagaata tatcagagcc tttaaaatct attgttaata
      841 ataaagaaat aatagtttta tctaccccag tttataaaag tgggaaaata gttggtgttt
      901 taagtggaat ttatgattca aaaaagttaa atgagttatt aggattatct gcatttgatg
      961 aaaaagcaga tgtttttgtt gcaaagaaaa atggagatat agttgctcaa aaaacaaata
     1021 aaaatagaaa gacgctatca tgtatatttc agctttttga caaagataat tgtgatgaag
     1081 atgattgtct aaaaactata aaagaaaata tgaaaaatga taagtctgga tattttaatt
     1141 ataacttaga taatgaaaaa tttatgttaa actatcagcc acttggagtt aatgattggt
     1201 atgttttttc tgttgtgcca aataaaatag tatctattca gtcagagaat atatcttatg
     1261 atgtatatat ttttgcatta gaaatactac tagttatatt gatttttata atatatatag
     1321 catgtattat aaataataac accaaaatta ttataaatga aaaagaaaaa tttaaagtac
     1381 tcactgataa tatattagga ggggttaaaa cctgtttaaa agatgaatat atgactatga
     1441 catatgtaag tcaggggttt ttagagctta caggttactc ttgtgaagat ttaaataaaa
     1501 tatttaaaaa tagatttaaa gacatgattt ataaagatga tttagacaag tttaattgta
     1561 ttattctaaa tcaattagaa aaacaagata aaattgaagt tgaatataga attgtaagaa
     1621 aagatggaag tataatttgg attttggaga gaagtaagtc tataaatgat aataaaggaa
     1681 gtgagtgtat tcagggagtt ttaactgata taacagaact taagaaaata caagaagaat
     1741 tagattcaaa aagatttgaa atacaggcta tcaatgatag tattgttggt ggaatagtga
     1801 taactgagat aaatgatgat tttgatagta tatatttaaa tgaaggttat ttaaatatga
     1861 ttggatatac acgcaaacaa ctggaagatg agctaaataa taaattaatc aaacttattt
     1921 ttaaagatga tagaacagaa ttaattaata gtataaacaa acaactcaaa ttaagtgata
     1981 catttgcatc tgaacaaaga gttgaaaaaa gggatggcac tattatatgg attttattaa
     2041 aaggaagaaa gatgatttat gataatggaa aatctgtact ttgttgtgtt attgtagata
     2101 ttacttctgc taaattatta gagcaagaac ttaaaaagag tgaagaacgt catgaaatca
     2161 ttctaaagca gacgcaaaat attataatag aatgggatat agaaaaagat aaaatgaata
     2221 tatcacctaa ttggtataat aagtttaaat atgaccctat aactgaaaac gcaacaaaaa
     2281 atataattaa atcagtagat atatatcatg atgatatgga taagtttatt tcattaatta
     2341 aacttacttc acaaggtagt gagtatgaag agtgtgaaat tagacttaga actagagata
     2401 ctatttacgc ttggtttagt ataaaggcaa caactttatt tgataataat ggtaaacctt
     2461 ataaagtagt aggaagtttg actgatattg atgaatcaaa acgagaagct gaaaagttga
     2521 aagaaagggc agaaaaagac cctcttacag atctgtataa taaaaaagca tcacaatcac
     2581 ttatagagga gtacataaca ttggataaaa aaagaaaagg tgctttaatg attattgata
     2641 ttgatgactt taaaggaata aatgataatt taggacattt atatggggat gctgtattaa
     2701 gtgaaattgc aaatgaccta ataaatctat ttagagcaac cgatatagta ggcagaattg
     2761 gtggagatga gtttattgta tttataaaag atatttctga aacaagagat attataaata
     2821 aagcagaaga attggtaaaa acatttgaaa ggtctttttt aggtaaagga cgaaattata
     2881 aaatatcatt aagtgtagga attgctaagt ttccacaaga tggtatggac tatatgacac
     2941 tatttagaaa tgcggatata gctctttact cagccaaagg gaagggcaag aattgttata
     3001 cattgtatga taaaaaattt gataaagtcc aatatataac taatacaaga aatgcaggag
     3061 atagtgataa tgtagagatg tcttttaaag aacatatcac aaattatata tttgatatac
     3121 tatatgaatc aagtgatata gaagtggcag taaatttaat tctaagtatt gtaggtaaac
     3181 actttaatgt gagtcgcatg tatatatttg aaaattcaga tgataatcga gtatgtaaaa
     3241 atacatttga atggtgtaac gaaggtgtaa cctcggaaat taataatctt aaaaatattt
     3301 gttacaagga tttaggcaac tatcttttaa attttaatgc agatgggtta ttttattgta
     3361 atgatattga aaatttaggt agtgatgtga aaaaagtact taagccacaa ggcatacgtt
     3421 caatattaca atgtaaaatt ttagagggta atgaatttag agggttcata ggatttgatg
     3481 aatgttcata taatcgtatg tggactaatg atgaagtgaa cactttgaca tttatatcta
     3541 aaacgctcag tatgttttta ttaaaaatgc gtattcagag taagctagaa gagtctttaa
     3601 caattcaaaa atctgtgatg gataatatgg atatatgggc atatgttgtt gacaggaata
     3661 cctatgaatt actatttatc aataaaaaaa tgcttgagtc agcaccaaac actcatgtag
     3721 gtgatttatg ttataaagcg atatggaata atcaagataa accttgtaaa aaatgcccta
     3781 tgatatattt aaaagatggt gatgatactt ataaatcatt catgcataat aaaaagtttg
     3841 aggtacaggc aaatataact acaactactt tgaaatggtt tgatggtaaa gatgcatgtt
     3901 taatatttgg aagtgaaata actagtattg aagctgaaga aaatacttaa aatactgtgt
     3961 tatatatata cttaaaatcg caatttgaat ttattagact taaaatgtct atataaatca
     4021 tttaagttgt ggttttaagt attttttatg atataattaa gcgttaagaa caaaattaaa
     4081 agatactttt gtataaaaaa atatttgtta aatatatatt gagcaggtga taatatggaa
     4141 aataagtttt taccaataag caaacaagat atgatagata gaggatggga agaattagat
     4201 tttgttctag ttacaggaga tgcttatgtg gaccatcata gttttggaac agcaataata
     4261 tctagagttt tagaaagtgc tgggtataaa gttggaataa tagctcaacc agactggaga
     4321 actacagatg attttatgaa gctaggaaaa cctagacttg catttctagt taatgcagga
     4381 aatatggatt caatggtcaa tcattattct gtaagtaaaa agcatagaga aaaggatatg
     4441 tattctccag gtgggaaaat gggttataga ccagatagag ctactatagt atattgcaat
     4501 aaaataagag aagcatacag tgatgttgca atagtaatag gtggaattga agcgagctta
     4561 agaagatttg cacactatga ttattggagt gataaggtta gaaagtctat gttaatagat
     4621 agtggagcag atttattagt ttatggtatg agtgaaaagc agattgtaga agtagccaat
     4681 gcacttaatg atggatatga cccaaaatac attagacata ttgatgggac atgctatatt
     4741 tcagatacat tggaagaaat atatgataaa tatatattaa ttccatcata taaagaaatt
     4801 tgtgaagata aaatgaagta tgtagaagct tttaaaattc aatatgatga gcaagaccca
     4861 tttagaggta atataattgt tcaacctcat ggaagtaagt atctggttca aaataagcca
     4921 gaaaaaccac ttagtagaga agaattagat gaagtatatg gattaccata tcagaagaca
     4981 taccatcctg tatatgaaaa atttggagga ataccagcta tagaagaagt aaaatttagt
     5041 atagtaagtt caagaggctg ttttggtagt tgttcatttt gtgcaataac atttcatcaa
     5101 ggtagagcag tgcaaagtag aagtgaaaaa tcaattattg atgaagcaat aggaataaca
     5161 aatttagatg attttaaagg gtatatacat gatgttggag gtccaacagc taattttaga
     5221 agaccagcat gtaaaaaaca aattactaaa ggtgcttgta aaaatagaca atgcttatct
     5281 ccatcaccat gtaaaaattt agatgcagac cacagtgaat acttacattt acttagagct
     5341 gtaagaaaat tacctaaaat aaaaaaggtg tttgtgcgtt ctggaattag atacgattat
     5401 gtaatggcag ataagaacaa taaatttctt agagagttga tagagcatca tgtaagtggt
     5461 cagttaaagg tagccccaga acatatatca gaagaagttt taaagtatat gcaaaaacca
     5521 gcaggaaaaa cttatgataa atttagacag aagttttttg ctataaatga agaattaggt
     5581 aaaaagcaat atttgatacc ctatttaatg tctagtcacc ctggttctac cttaaatagc
     5641 gctatagagt tagcagagta tttaagagat actcactacc aaccagagca ggttcaggat
     5701 ttttatccaa ctccagggac tctatctaca accatgtttt atacaggtat tgacccgctt
     5761 acaatgaagc cagtttatgt tcctaaatca aaacgtgaca aggcaatgca aagggcttta
     5821 ttacaatata gagcacctag aaattatgat ttggtatact ctgcgttagt tgaagcagga
     5881 agagaagact taattggttt tggacataga tgtcttataa agccaaaaaa tgaaaagcct
     5941 tattttaata gaaataattc taaaaaaaat gtatctaaag gaacaaataa aaacaaaaaa
     6001 actaatacaa ataacagaag taataaaaat caacaaaaaa gttcaactaa aaaaaagaaa
     6061 aaacattaaa gtataaataa aaatacttaa aaaagaagga ttattcgata aattaacaga
     6121 aattagtata aataaatata attattttaa tgacgttacc tttgggagga agagatgaaa
     6181 aagtatattt ctataatatt attagtggtg ttgactatgg ttgttttggt aggatgttca
     6241 ccaggaaaag ataaccctaa aaataaggag ctatcagttg tcaaacaagt aaaagtagct
     6301 gttccagatg gactaccagc agtggcaata gctaaacttg caaatgaaaa tccagagatt
     6361 aaagaaggat atgaaacagt gtatagcatt gaaaagacac ctgaagcgat ttccactaga
     6421 gtgatgaaaa aagatgcaga tatagctata gttccttcaa acatggctgc aatagcgtat
     6481 aataaaactt catcatataa tattgttgga actgtaggaa tgggttcact ttatttggtg
     6541 tcaacagaaa atataaaaga ttactctgat ttagttggta aagaagttgg atgtactgga
     6601 aaaggactta ctccagacat aactataaaa agtcttttgt tacaaaagga tataaattat
     6661 tcagatataa aatttaatta tgtaaattca gcaagtgaac tggtaccatt attggctact
     6721 ggtaaagtta aaacaggtat tgttccagaa cctgcactta gtgctttgat gtctaaaaat
     6781 ccagacataa aaattataaa aagtttaaat gactcatgga aggaagtaag tggttcaaaa
     6841 gatggttatc ctcaatctac tttaattgta aagtctgaat ttttaagaga taataaagat
     6901 tttgttgatt cttttgtagg tcaattatca aatagtatag attgggcaaa caaaaatcct
     6961 gaagaattag gtgcatattc tgaaaaaata aaaatatcta ctgaatcaaa gataatagga
     7021 aaatctcttg aaagagctaa tttgaaatat attccagtta aggacatgat taaagattat
     7081 aaaaattact atgagaaatt agctaatttt gatgataaga cattaggagg caaagtacca
     7141 gatgaagcaa tatactttgt tgagaaataa aaaaaataaa gaacaattta tagataaaat
     7201 ggaggtaaca ctttcttgtg ttatcctctt atttttatgg caaattattg ctttaaaaat
     7261 tgataatgat atttttttac caacagtagg tcaagtgttt aatagtataa aagagattat
     7321 gctaaattct agtttctata tagatatact ttactctatg gggagatgtt tttttagttt
     7381 tttactggct atgatttttg ctattatact ttcaatagtg tcttatttaa atagattttt
     7441 tagaaactta ttaaagccaa tcaatgcttt gacacgttca ataccaacta tgatattggt
     7501 agtacttgca cttatttggt ttgaaaagga tagtacccct tttatagttg gatttactat
     7561 agtttttcct atattgtatg ataacgtttt gggcgctata ttgaatatag acaagaactt
     7621 attagaaatg gctaatgtgt ataaaataag gtttatagat aaggtgttaa aaatttattt
     7681 accagccata aaatttcaaa ttatatctat actagtatca acattttctt taagtttgaa
     7741 agttgctata gcaggagagg tgtatagtca acctacatat ggtattggaa ctatgataca
     7801 gactgaaaaa attaacttta acacatctgg aatatttgca tggataatta ttgttctttt
     7861 aatatctgct attttacaaa tagctcaaaa gtttttagca aggagagctt ttttatggaa
     7921 aagataaagt tgagtattga aaatataaat aaaagatatg atagtcgtat tatatttaga
     7981 gattttaata ttgattttta tgtaaatgag gtaaattgta tccttggaaa atctggttgt
     8041 ggaaaaacta cactcttgaa cataataagt gggattatta aaaatgacac caacaatcta
     8101 aatatcaaag aaaatttaaa tagagtagga aataagcttg aagctagtta catatttcaa
     8161 gatgatagac ttatagactg gttaacagta gaagaaaata taaagatagt tgtaaataaa
     8221 tactataata aaactcaatt aaataaaatt tgtgatgaat acttggaatt ggttggaatt
     8281 agtgattata aaaaattcta tcctcaaatg cttagtggag gtattagaca aagagtaaat
     8341 attgctagag cttttatata tccatcaaaa aacattataa tggatgaacc ttttaaatct
     8401 attgatgcaa agaatacaca gcttataatg gataatttta gaaatatatt aagaaaagaa
     8461 aagagaactg tgttatttgt aactcataat attgaggaag cattgttttt agcagatagg
     8521 atatttattt taggagactc tccaattaga ataaaaaaaa tattaaaaaa ttctaaggaa
     8581 ttggaaaaaa atgaagtttt gaagttgata tagagttata ataatactgt cgttttaaaa
     8641 tatatttggt taaaatctat aagactatat taaaatagta cagaatattc aaaactgtgt
     8701 ataatgttcg tattttataa aaaggaagtg aatgtatggt taagactaat gctatgagaa
     8761 tattagattc aaataagatt gattataaag taatgtctta tgaagtgaaa agtgaacatg
     8821 tggatggtgt tgaggtggca catgatatag gaagagatgt aaatgaagtg tataagacat
     8881 tagtaactca aggagttagt aagaatatat atgtatatgt aataccagta catgaaaact
     8941 tagacttaaa aaaggctgca aaagtagcta aagagaaaag tgtagagatg attcatgtaa
     9001 aagatataaa taagcttaca ggttatatta gaggcggatg ttctcctata ggtatgaaaa
     9061 agttttataa aacttttgta aatgaaagtg caaagaattt agatacaatt attgtgagtg
     9121 caggaaaaat aggatatcaa atagaactat ctccatttga tttacaaaga cttataaaag
     9181 tagaatttgt agatgtaata aaaaaataag aattacatat aaaatataga gcttatattc
     9241 tcaataatag gtgttgtaat attaataaaa tgaataagaa ttttaaaata aatattcagt
     9301 tacttatgtg taattggtat aaaaatattg agaaatttta aaaacaatgg taaagtgtaa
     9361 gtataatcac gaatattttt tataaagtaa tggagggatg ttcgcttgag aaaaataaaa
     9421 tctgaacaaa tcgtcgagca ggtaaaaaaa ctttgtatag aagcgagcct atatttagga
     9481 gaagatgttt taagttgtat aaaagaaaaa gcgaaaagcg aaaagagtga agttggaaaa
     9541 aatatattga atatattagt tgaaaatgct gaaatagcta aagaaaaaaa tataccaata
     9601 tgccaagata ctggtatggc tgtatttttt gttgaaatag gacaagaagt tttaattgaa
     9661 ggagacacac ttactgatgc tataaatgag ggagtaagac aagggtatga agaaggctac
     9721 ttaagaaaat ctgttgtaag tccaattaac agagtaaata caaaagataa tacacctgct
     9781 gttattcatt atgatatggt aaaaggtgat aaaattaaaa tagaatttgc agctaaggga
     9841 tttggaagcg aaaatatgag taagatgaaa atgttaaagc catcagatgg attggaagga
     9901 attaaaaaat tcataataga tacagtttca gaagctggac ctaacccatg ccctcctatg
     9961 gtaattggag ttggtatagg tgggactgtg gacaaatgtg ctcaaatagc taaaaaggca
    10021 ctttttagag aactaggtga atttaataag gacgaaaata tagcaaaact tgaaagtgaa
    10081 ctattaacag ctattaataa acttggtata ggacctcaag gcttaggtgg tactacaaca
    10141 gctttgggat taaacataga gacttttcct acacacatag caggtctacc tgttgttgta
    10201 aatataaact gccatgcatc aagacataag aatgtagtaa tttaggagga aaataaaatg
    10261 ataaaaataa ctacacctgt aaatgaaatt gatatagcaa aattaaattg tggagataca
    10321 attagtttga gtggtatact ttatacagca agagatgctg ctcataaaag attgatagat
    10381 tgtataaata aaggtgaaga attaccattt gacgtatatg gtcaaggaat atattatgta
    10441 ggacctactc ctacaaagcc aggagaagtt ataggtgctg caggtccaac gacgagttat
    10501 agaatggatg atttgactat acctctttta gaaagaggtc ttagattgat gataggcaag
    10561 ggaaaaagaa gtgatgaagt tatagaaggt atgcaaaaat acggagcagt ttatcttgct
    10621 gcaataggag gagctggagc gtatatttca aattcaataa aatcatgtga aattattgca
    10681 tatgaagatt taggagctga agctataaga aagattgtag tagaagacct taagcttaca
    10741 gtagcaatag atagctatgg aaataatata tatgagcaag gtcgagcaat ttatgaatgt
    10801 aaataatgtt tagaaataaa tatgaatatc aaaattaaaa atagttgtgt atattttaag
    10861 gaggaaaatt gatgtctaag gattatgcaa aattggcatt agaaatgcat gaaatcaaca
    10921 aagggaaagt aagtgtagaa agcaaggtag aaattaaaac taaggatgat ttatcaacag
    10981 catatacacc aggggttgct gaaccttgtt taaaaataca tgaaaatcaa gatgatgttt
    11041 atagatacac ttctaaaggg aatctagtag cagtagttag tgatggaagt gctgtacttg
    11101 gattaggtaa cattggtgct gaagcatcta tacctgttat ggaaggtaaa gctatattat
    11161 ttaaacaatt tgcagatgta gacgcttttc caatatgttt aaagactaat gatgtagatg
    11221 agatagttaa atctgtagag ttgatggagc ctgtatttgg aggtataaat ttggaagaca
    11281 tatcttctcc aagatgtttt gagatagaag agagattaaa aaaatcttta agtatacctg
    11341 tttttcatga tgaccaacat gggacagcaa taatagttgc agccgccata ataaactcaa
    11401 taaagttaat tgaaaataaa aaaatagaag atttagaaat agttataaac ggagcaggag
    11461 ctgctggtat agccatagca aagatactac ttaatatgaa tgttaaaaat ataatcttat
    11521 gtgatagaag tggagcattg gaagctagta tagaaaatct aaactatgtt caaaaagaaa
    11581 tgttaaaagt aacaaatata agaaatgaaa aaggaccttt aaaagatatt ataaaaggaa
    11641 aagatgtatt tattggtgtt tcaggtccag gagctgttac aaaggaaatg gttgaaagta
    11701 tgtctgagaa gccaataata ttggcaatgg caaatcctac tccagagata atgccagaag
    11761 aagcaaaatt aggtggagca tttataatgg gaacaggtcg ttcagacttc cctaatcaag
    11821 tcaataatgt actggctttt cctggaatat ttagaggtgc acttgatgtt aaggctaaag
    11881 agataaatga agaaatgaag atagctgctg catatgcaat tgctaataca ataagtgatg
    11941 aagaaatatg cccagagtat atattaccag atgtatttaa taagaatgta gtaaaaaatg
    12001 ttgctaaggc agttaaagaa gcagcgataa aaactaaagt aaatagaata taaattttta
    12061 tatgttcata tatccttata ttatcgggta tatgggcatt tttattatag gtttatactc
    12121 atatatcctt acttttatat acatatcttt atgattaaag gtgttagaaa atgtagtaaa
    12181 ttttatacta tcagactcta tctttattac tttagtagat acatggttgt aaaggtttat
    12241 agaaaaaaat ataggaaggc agatttttat tgttgcgtat atatgagaat gatataatat
    12301 gaatattata aattaataaa tttaaattta tggaggtaca agagatgatg gaatataaag
    12361 ttaatgacca agaacttaat gcttcaatgt ttatcccatt cgtcaacagg atatggccag
    12421 gagattatga tatggataaa acacagactg cattatcaaa aactttgaat ataactgctt
    12481 atgatgataa agtgcttgta ggatgtctgc gagttctttc agatggttat tattttggaa
    12541 caattacaga attgcttgtt cttccagagt atcagaaaca aggcattggt agtaagcttt
    12601 tccagcttgc taaagagaac accccaacta tgctatattt tggtgcacag ccaggagtag
    12661 aagcgttcta tgaaaaaaat gggtgccaaa gaagtctgca atcctacata ataaaaaaga
    12721 aagatagtta ttaaagttcc agctttcaat aatcttaaaa gtacaatatt ttagtggaat
    12781 catttcattt tttattacta atatgacata aacatgtcat attagtattg atatactttt
    12841 aatatggtta agagccaaag aaaaatataa ggagtgattt aaaatggaag aaactaaatt
    12901 ttgccaatgc tgtgcaatgc caatgggaca aactgatgaa ttatatggga caaataaaga
    12961 tggaagtaaa agtactgatt attgtagtta ttgttttaaa gatggtgagt ttacagctga
    13021 tataagtatg gaagagatga tagaagtttg tatccctcat atgcttcaaa gtaataagga
    13081 tatgactgaa gatgaagcaa gaacaatgat gaacaatttc tttccaacat taaaacgctg
    13141 gaaataattt tatgcaaaat tagttatatg caaaacatga aatatggtgt tgagaataag
    13201 gggtggtgtg ctttatagca taccacctgt tttgttactt ggagttcagt tattttccta
    13261 aatattttaa atatactatt ttagataagt tccctcttca tcttgataaa ttaatccttt
    13321 tttcataagc actccaagag ctcgtttaaa atttttctta cttgaattaa ataatataga
    13381 aatatcttta gggtcagatt tatcattaaa tctcatatat ccatcagaac cttctaaata
    13441 acttaaaatc ttattttcta aagtatcaag ttcatccttt ctatttcctc taggagaaag
    13501 accaagtttt ccatcttcat atatttttat gacatgtaaa ttttcaagaa catcaccagt
    13561 ttttagttca gtaaagtact cattatgaag tattacacca gcatatttat tgtctacaca
    13621 tatcattgca gaattattag tttggaatcc atatacaact ccagttacaa tatctcctac
    13681 attgtaagta tggtctgtta ataagtatga atctatgtct gtagtagcag caattctttt
    13741 gcttttgtct aaatatatat aaaataaata tttttcgtct ctaaatagtg gataagtctt
    13801 agctttaaaa ggaactaata tgtctttagg aagtcccata tctataaaag taccaatgtc
    13861 tgtattatca actactttta aatgtgctac atccccaacc ttagctaaag gcggtattaa
    13921 agttgcagca gttctgtcat cagaatcttt aaatatgaaa gcatttactt catcacctat
    13981 gtttatttca ttttttccga ttaatctatt atgaagaagt atatcgtctt ttgtgttatt
    14041 agtttgtcca tccaaaaaat atccaaattc agtttttctt tttactgtaa gtttattaaa
    14101 atcacctatg tttatcaaat taaaatcatc ctctcatttt atatttataa tttagccata
    14161 atatacttat gtagtagttt gtcacagtat ccatattagt attagtgaaa tttacaataa
    14221 ataatatagt tatttatgta tttttcaatt ctctagcttc ttatatagta taaactccaa
    14281 tttctttttt gtctttaggt tttattaaaa tttttacaaa ataagttaaa aagtattgaa
    14341 ataaaatttc ttttaaagta taattggtat ataccaaaat tggtatatac catatcgaaa
    14401 agtggtgact atatgaaaat agtaaataaa tctagtaata taccattgca tactcaatta
    14461 agttcaataa taagagagat gatagaaact ggtgagctta aagagggcga tgcaataatg
    14521 ccagaaagag aactttgcaa catacaaaat gtaagtcgta tgacagtaaa taaaaccatt
    14581 gttggattag ttacagaagg acttctttat agggtacaag gtaaagggac attcgttgca
    14641 aagcaaaaaa agaagtatca gttttcaaat gtaaaaggtt ttactgatgt tatgaaagaa
    14701 aagggagtaa acataaaaac ggatatatta tcctttgaga tggaactacc agatgattta
    14761 gttaaaagaa agcttggaat aagtgataat actacaaata tatataagat tgtcagactt
    14821 agatatactg atggagagcc atttggatta gaaatagtat atctatcaga agaaatgtgt
    14881 aagggtctaa caaaagggat attggataat tcttcattat atagggtttt aaatgaaaaa
    14941 tatggctata agatacaaaa agctgaacag gtaatggagc cagtaatact ttcagatgaa
    15001 gaaagtaagc ttcttgaaac agatgaaggt gctttagctc ttaaacttca tagaaattcc
    15061 tataatagag aaggtagccc tatagaatat accatatcaa tatttagaac agataaatat
    15121 caatatgaaa tagtattgag tgagtaggtg aataatatga aatgtttaat taatggaaag
    15181 ataatattaa aaaatcaaat cttagaaaat aaagtattag tatttgatga aaaaataatt
    15241 gatatagctg atagtgtacc aaaagattgt gaagttatag atgctgatgg aaaatatata
    15301 tctccagggc ttattgatat tcatatacat ggaaacatgg gaaaagatac aatggattcg
    15361 actgatgaat ctattgaaac aatttcaaag tcaattatga gacatggagt aacatctttt
    15421 cttccaacta caatgactat ggataaagaa catgtttatg atgcacttga agttattaaa
    15481 aaagcccaaa atagaaagct tgaaggggca caagtattag gagcacattt agaaggacct
    15541 tttataaatg agaattataa gggagcacaa aatgaaaaat ttataataaa ttcaaaatac
    15601 gaatttataa aagaatataa agatgttata aaagttataa cttatgctcc agaaaaagat
    15661 attgattttg attttactag agaaataaaa agatgtacag atatagtttt atctattggt
    15721 catagtaatg caaattatga ccaagctaaa gaagctataa atttaggagt aactaatgta
    15781 actcacatgt tcaatgcaat gactggttta aaccatagag acccaggcgt tgttggtgca
    15841 gcacttacaa ctaatgtgta cagtgagtta atagcagata caatccatat aaataaagat
    15901 ttatttcaat ttatactaaa taataaagga aaagaaagac tgattttaat aacagattct
    15961 atagaagctg gtggtcttga agatggcaat tattctcttg gaggtcagaa agtcatagtt
    16021 aagggtaatg aagctagact tgaaaatgga gcattagctg gaagtgtgtt gtctttaaat
    16081 aaaatggtat ttaacttctt agataataca aaccttaaag tgaatgaagc aattaattta
    16141 gcttctttaa atccagcaac atctttggga ataaatgaca aaaaaggaag cttagaaata
    16201 ggtaaggatg cagatatagc tgtatttgat gagaatttag attgtaaaat gacactttgc
    16261 ttgggggacg tagtatataa aaatatatag tatacaaaac aaaccttaaa gacagtaaaa
    16321 acaatataaa aaataataaa ttaaacttac attagtaaaa gtagtagtta aaatttagga
    16381 ggaataagat gagagtatta gtgtgtaaag attatgatgg aatgagtaaa aaagccgctg
    16441 aaatgatagc agctcaaata gttttaaaac caaattcaat actgggacta gctacaggaa
    16501 gtacaccagt aggtatgtat agagatttag taaaaaaata caatgataat atagtagatt
    16561 tttcagatgt gatgagcttt aatttagatg aatattataa gttaccaata tctaatgacc
    16621 aaagctatga ttattttatg aaagaaaatc tatttaatca tgtaaacata aagccagaaa
    16681 atactcatct accaaatgga atggctgatg atattgaaaa agagtgtatg aactatgaag
    16741 cttctataga tgcagctggt ggtatagatg tacaagtact tggaataggt cgtaatgctc
    16801 atataggttt taatgaacct gatactaaat ttgcaaagag aactcatgta gttgaactta
    16861 cagagtctac aatagaagct aatgcaagat tctttaaatc aagagaagat gttcctaaaa
    16921 aagctgtaag tatgggaata ggttcaatat tgaagtctaa gaaaatattg cttttagctt
    16981 caggagaaga aaaagctgat gcagtatata atactgttta tggagatata actccagaag
    17041 ttcctggttc tatacttcaa cttcataaag acactatagt tatagtagat gaagcagcag
    17101 caagtaaatt gaatccaaaa gattataaat tagtataaga atagaatgta gtttaagtaa
    17161 aaaatagcta tcattgagta atcaaattta tttaatgata gctattttta tttaaatttt
    17221 ttacttcttt ttatttaaat tagttattat tgtttaaatt acttgcttat tactttttac
    17281 gaaaactcgt tatcaagaag tatccaaata agatgttaaa aattattatg tttagtggga
    17341 ttctatcaag cataataaaa ttacctataa ttgggatatt accaactgga aacataaaga
    17401 agaatacagg catcagtgat agtaaaaaag atggaaaaca aagaagaagt aatcttggaa
    17461 tatcaatata ccaactacca tcctttttgt attctctaat caataattca gttcctagta
    17521 ataaaccaat accaccagag gaaattaaat taataaaaat gtatctttta aagtcttgca
    17581 tataaccagt gtaaaattga gtatagaaat cataatttaa tttaacagct aaaaaataca
    17641 taagcaatag aatgattaaa aataaaagat atctaagagc ttttttcatt ttaaattctc
    17701 ccttcagaaa tactttattc agttttattt tgttggaatc tgattttatc atttagttta
    17761 agaaatccat aatattagtg aagaatgaaa taagtattat atatgaataa atatttttag
    17821 aattaaggtg aattgtagtg cttaatataa ctataatatt aattgtttga atattcaata
    17881 aaaagtttga aaaatatcaa ttttgatata aactgaataa atacagttga atattaaatt
    17941 attattttat caaaaatatg caaagtacta caccttcaaa tatcacctaa a
//
LOCUS       DAYAPA010000083        17863 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.083, whole genome shotgun sequence.
ACCESSION   DAYAPA010000083 DAYAPA010000000
VERSION     DAYAPA010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17863
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.083"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            375..1304
                     /gene="glsA"
                     /locus_tag="ACGI59_002577"
     CDS             375..1304
                     /gene="glsA"
                     /locus_tag="ACGI59_002577"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFG9740359.1"
                     /translation="MNTLDETLLKEIISSNKKYTNYGQVASYIPELKNARRNDLGICI
                     IDSENNLYSAGNCSTKFTIQSISKPIVLAMALMDNDWEDVFSNVGMEPSGDPFNSIMK
                     LEINDTKKPCNPMINAGAIVTTSLINGSCLEEKEERMLSFFRKLAKNDNIGINYDVYK
                     SEKMTGDRNRAMAYLLKSDGFIRGNVEDVLDLYFKQCSIEIDSVDLARIGINLANYGV
                     DIENGEHLMSEMVSRIVKTFMMTCGMYDASGEFAIKVGIPAKSGVGGGIMASVPGRMG
                     IGVYGPALDKKGNSVAGVKVLEELSNKLKLNIF"
     gene            1402..1965
                     /locus_tag="ACGI59_002578"
     CDS             1402..1965
                     /locus_tag="ACGI59_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFG9740360.1"
                     /translation="MKEVLVLRDLECIKAIAHPRRIDILKAFDKSPLSAKQLSQLLEE
                     PHAKINYHIKMLYKVGILELVEEKIKSGIVEKYYYPSAKNVVIGNRILNFSLDNGEEK
                     EELYISKFENMSEVFYKAAEEDVLENENIVDYHDISLTHDELVELSDTMKSKIDEILN
                     KRQHNVEGSKYDIAMVIVPTLEEECPS"
     gene            2453..3289
                     /locus_tag="ACGI59_002579"
     CDS             2453..3289
                     /locus_tag="ACGI59_002579"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008833 - deoxyribonuclease IV
                     (phage-T4-induced) activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFG9740361.1"
                     /translation="MIKLGTHLSIAKGFANAAETAVYIGANTFQFFSRNPRGGNAKEF
                     DEKDIIKFQEIRKENNFAPLLAHAPYTMNLGGTKDDVYEFATRVIDEDIRRMDSLGIE
                     YMCFHPGSHVGGGVDFGIDRIVKGLNNAIKGDENITILLETMSGKGTEIGFKFEQLKS
                     IIDKIEHKEKIGVCLDTCHIFSAGYDIVNDLDGVLEEFDKVIGIERLKTVHLNDSMMP
                     FGDKKDRHAPIGEGKIGLKALIDFMEHPSLKHLPFFLETPFDDEGHKRELKMIKEILS
                     SK"
     gene            3573..4568
                     /locus_tag="ACGI59_002580"
     CDS             3573..4568
                     /locus_tag="ACGI59_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFG9740362.1"
                     /translation="MRNLGNTNMKIKRVGFGGIPIQRITQDDTNLVINELEKQGINFI
                     DSARGYTISEEAIGIAIEGKRDKFFLATKSMSRDYDSMKRDVEISLNNFKTDFIDLYQ
                     FHNVKEEEYDNLFKDKMAYSALLEAKEQGKIKHIGITSHNLNTIEKAIEDGKFDTIQF
                     PYNIVEGQADEVFKKAHEKGIGIIVMKPLAGGALDNATLAIKYILSKEYIDVVIPGME
                     SVEQVRQNVAVLENLVLDEKDNKEIEEIRNSLGKKFCRRCEYCMPCAVGINIPLSFLC
                     EGYYTRYGLKEWAKEKYEVMDVKPTECIDCGLCESRCPYELPIREMLKTVVEKLG"
     gene            complement(4825..5229)
                     /locus_tag="ACGI59_002581"
     CDS             complement(4825..5229)
                     /locus_tag="ACGI59_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFG9740363.1"
                     /translation="MIKITIPGRPISKSNFKLHNINGQAWMPSKGKYSKYLAYENMIA
                     GFINQQYQGETVEENLITVLKLFFPNKRMGDLHNYPKSICDGIEKSGIIKNDKQLKPV
                     LLFDFIDKDNPRVEIELYPVSKYDISYNIFEK"
     gene            6251..7165
                     /gene="queG"
                     /locus_tag="ACGI59_002582"
     CDS             6251..7165
                     /gene="queG"
                     /locus_tag="ACGI59_002582"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFG9740364.1"
                     /translation="MDNKELKAFCNLIGLNCVGVAGVDKYDNLEKILKDRQQKGYLTG
                     MEEPIIENRINPRHIMKDANSIIVCAFPYHINRQEKNKNSNLSKYCHGKDYHIVVKDF
                     LQQICDYISKTVTDFKYKLFADNGPLVDRYLAYLSGIGYFGINNNIITDEYGSYVFIG
                     YIVNNYKFEEDKPLEKSCFKCGKCVKYCPGNAILGNYDMNPKKCLSYITQKKGDLSEK
                     EKVVLKNGKKVFGCDICQEVCPHNVEIPTTHILEFKENIIDYLNYDEIQNISNKEFKR
                     KYGDRAFSWRGRNIIKRNMEIILDKYDD"
     gene            7271..9508
                     /locus_tag="ACGI59_002583"
     CDS             7271..9508
                     /locus_tag="ACGI59_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFG9740365.1"
                     /translation="MKYNNTSEINPLDYILGQERAVEAMELGLKINNPAYNIYIAGEP
                     GTGKSTYALKVLNEYASKKNTHKDWCYIYNFENPREPIIVELEKGFGRELKKDMEKLI
                     ESLLEDFKNAFESENFEIEKNKLLDEYEIEKDMLLKQIKKYGEEKGFKLKQSKMGIVF
                     IPLKEEEDESDESDEEFYKAKRELENMAIQVVYKIRNLEEIAEKAVLELEEEIAKLVV
                     EPHIKRLCEKYENYDKIQTYLENIKKDIIEYMYLFYLDEEELKDKYDKEHFIKYKINL
                     FVDNGSSKNKESAPVVVEINPSPANLFGKAEYDYANGNIKTDFTKLLSGAFHRANGGY
                     LVLYADQLLKYTMSWEILKKTLQTKKVILETQTAIKPENMPLDVKLVLIGSHYIYDIL
                     YRYDEDFEKYFKVFVDFDSEMDKNEDNEEGIARFIAYQCDKNNLRHFTKSAVEEVIKF
                     STRLSGDLEKLSTKFNKIMEVVIEGSAYAEFRNSEYTEQCDVKKAISEKRKRINRVET
                     HMDESIENGFTLIETEGRKVGVINGLSVLSTGEYSFGRASRITATTSPGSKGIVNIER
                     EVNMSGSIHNKGVLILGGYLSENFAQDLLLSLNAYVCFEQNYGGIDGDSASSAELYVL
                     LSSLSGVSIKQNIAVTGSINQKGDIQVVGGISEKIEGFYSICKTKGLNGEQGVIIPRK
                     NQRNLVLSDEVNDAVRDGKFKIYTVERVEEAIEILTDVKFEEIKKLVKEKLISFSKIQ
                     TVSKE"
     gene            9706..10827
                     /gene="nth"
                     /locus_tag="ACGI59_002584"
     CDS             9706..10827
                     /gene="nth"
                     /locus_tag="ACGI59_002584"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFG9740366.1"
                     /translation="MKNVNLKEINEKDVILEEEINKKGMDKKNIIEKEEINKKGIDKK
                     DIIEKEEINKKGIDKKDIIEKEEINKKGMNKKDIIEKEEINEKGIDKMIGKKDEIIDE
                     KDIENFMDIEEDKKSIKVSKKSTKKGETEKKTKKSSSNKATQKTSKETKSKSKIKNES
                     ANEKDVNKILDELEKLYPDAKCELNYGTAFELLIATILSAQCTDVRVNKVTSELFKKY
                     NTARDFANLSIEEISKEIKSCGLYKSKSQKIKDTSEQLCELYDGEVPDSLEKLIKLPG
                     VGRKTAGVVLSNAFNHPAIAVDTHVFRVSNRIGIVDEPNPQKTEFALMEAIPKERWSH
                     SHHVLIFHGRRMCKARNPECASCPIKEDCNYYKELNETK"
     gene            11021..11491
                     /gene="trmL"
                     /locus_tag="ACGI59_002585"
     CDS             11021..11491
                     /gene="trmL"
                     /locus_tag="ACGI59_002585"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFG9740367.1"
                     /translation="MSLNIVLVEPEIPQNTGNIIRTCAATGSTLHLVKPLGFSLDDKH
                     LKRCGLDYWDIADIQYYDSFEELQEKYPNSKYFFSTTKAKQSHSDVKYEENCFIVFGK
                     ETKGLPEPLLKANMDTCIRVPMLDVEKARSLNLSNSVAIVVYEALRQIGYPNMR"
     gene            11527..12375
                     /locus_tag="ACGI59_002586"
     CDS             11527..12375
                     /locus_tag="ACGI59_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFG9740368.1"
                     /translation="MKNLKIICDSLSDVPKDLLDKYDIDIVSLTVILEEREYKDRIDI
                     SGDEFYKRLREENVYPKTSQATYAQFKEIFEKYTNEDREILYIAGSATATGTYQSAVM
                     AKNDVDGKIYTYDSNLLCMGTGLLVIKAGELASEGKSVAEILPVLDELKEKAFVIFSV
                     DTLEYLQKGGRISSTKAAIGSILNIKPILEIRDGLVSQISQVRGKKNVISKMMELIKS
                     NCGDDLSDQIVYIGYSDDFKEKEQLSKVMQDELRPKEIRFFQIGVCIGAHAGPGVTGI
                     ICLKNK"
     gene            12663..13667
                     /locus_tag="ACGI59_002587"
     CDS             12663..13667
                     /locus_tag="ACGI59_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFG9740369.1"
                     /translation="MRFKRILILMLTVAMIAGMLVGCANNPSNNPSSSKDNKSDISDK
                     SNSKKSVSMILDIEGTNNEAMNNSALLALNNAQKKLNIDTNKVESDDSSTFSNSIDIL
                     CNDNYDLIIAVGARFAKPLEMVAKKYPKQQFAIIDYEYDKQPSNITSISYEDNKSGYL
                     AGLIAGKMTESDKVGFIGGTKSSSRDKFESGFREGVKFSNSSIKDISVEYADVFKDSK
                     SVESIAKKMMDNGVDIIFSTTEDDSKAVIDAVRAKNKKVIATNKDQHELAPENVISSI
                     VKDFENPTYNLIQSFVKGNYKGGKVIENTVKSGSTGLAQNSSQNIPPDVLEYVNKNNK
                     "
     gene            13793..14905
                     /locus_tag="ACGI59_002588"
     CDS             13793..14905
                     /locus_tag="ACGI59_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFG9740370.1"
                     /translation="MKIKRILMACFVVVFTIFILAICNFDFGFDKVNKNNSNIDSAKS
                     KQSESKSLLTSAEKDKSNSKSKNKEDDTEDDSYVRKTIGVLLDTENLNNESYNNNVLS
                     ALKRADEDFDVRIKLEKPKTSKSFKESIEKLHKYEPDFIIAVGARFAEPIKEAATKYP
                     KQQFAIVDYTYKVQPANVISVSFQDNVTGYLAGLIAGKMTDTGKVGFIGGVEGSSRDK
                     YESGFRKGLKTSNKDAELLVKYSNIFENPESARTIAKEMRDSDRDVVFSATEDDSKRA
                     IQVARENGGKVIAINKDQYDMAPDNVISSIIKDVEQPVYELIKNVAKDGFKGSKVMEF
                     KIKDGELGLTSKSSRNIPPDVLEYIKKEYNKVKDTY"
     gene            15395..16156
                     /locus_tag="ACGI59_002589"
     CDS             15395..16156
                     /locus_tag="ACGI59_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6873 family GME fold protein"
                     /protein_id="HFG9740371.1"
                     /translation="MEFTREPFILDDDLCLALVDKRISYLMENELKLRNIEIIKTIEC
                     KELYESIKYHPDICICNLGKGDIIVAPNVYNQYKELLSRYKFNIIKGEANLLNRYPYD
                     IPYNIAIVGDYAIHNFKYTDKAILDYIENNKLKKINIEQGYSKCSICIVDRTSIITSD
                     KGIWKSMKNTEIECLLIEKGHINLFEMNYGFIGGCTGLISKDKLAFCGDVKKHPDYER
                     IKSFVESKNKEIVTLSCENLLDIGSIVPLMTRKER"
     gene            16159..16977
                     /locus_tag="ACGI59_002590"
     CDS             16159..16977
                     /locus_tag="ACGI59_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sporulation protein YtxC"
                     /protein_id="HFG9740372.1"
                     /translation="MVEKDNISSMKFLRNNNVNDRLIYTNDIVEVVIDSAKQEVLNKK
                     ILNGTKAEQRALNIDEISTEITSMIISIMKEKLLKEYILKCYGRTYPEDKNNIYIYSL
                     NIFAKKEIFMRETVFTRVRNYIEENDFINVEGFIRFRMREFMKYISAIGDIAVEEYLI
                     KKDQDEFIRVLKYFIDTQEEKIDLLKVHIMEDNTFVLYDKNGNKIDSIDDEEIINMVI
                     RENLNYEDFLISTLLTLCPKKIEILDLLNNNCSKEIIDTVEAIFENKVSIIMEN"
     gene            17157..17759
                     /locus_tag="ACGI59_002591"
     CDS             17157..17759
                     /locus_tag="ACGI59_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 family protein"
                     /protein_id="HFG9740373.1"
                     /translation="MDNLIRILILAVIGGFIGYVTNVVAIRLIFRPIEPIKIPILNIE
                     IVGLIPKRRAEIAANVGEIIQEEFLSMDEILANIITDEDKEEVVRYIKARVKIIIHEK
                     VSFIPSGIKNMIQDYLGEIIESEVKQSIDELSKNIINKANERIDIQKMVEDKINELDL
                     YELEEIIIRIAKKELKHIEFLGLVLGFLIGIAQGLITMFI"
BASE COUNT         7372 a         1613 c         3286 g         5592 t
ORIGIN      
        1 ttactaaatt actaggttgt taagttactg gattattaga tagttagatt actaggttgt
       61 taaattgtta aattattgaa ttgttagatt gttgaattac taaattacta ggttgttaag
      121 ttactggatt attagatagt tagattatta gattgctaaa ttattaggtt gttaaattac
      181 tggattatta gattactaaa ttgctaggtt gttaaattat taaattatta agctatttaa
      241 actatttaaa ttgtttgaaa aatgaattga ttcttgtaga aaataatttt aaagtataat
      301 tttttttaac attgttttat tatagtataa acttgtgcta aaatagaata tataacataa
      361 tataagaggt gcatatgaat acgttagatg agacattatt aaaagaaatt ataagttcta
      421 ataagaagta tactaattat ggacaagtcg ctagttacat accagaatta aaaaacgcta
      481 gaagaaatga tttaggaatt tgtatcatag atagcgaaaa taatctttat agtgctggaa
      541 actgtagcac aaagtttact atacaaagta tttcaaaacc aattgtgctt gctatggcac
      601 ttatggataa tgattgggaa gacgtttttt caaacgttgg aatggagcca agtggggacc
      661 catttaattc aataatgaaa cttgaaatta atgacacaaa aaaaccctgt aatccaatga
      721 taaatgcagg ggctatagta accacttccc ttataaatgg gagttgttta gaagaaaaag
      781 aagaaagaat gcttagtttc tttagaaagt tagcaaaaaa tgataatata ggaataaact
      841 atgatgtcta taagtctgaa aaaatgactg gagatagaaa cagagctatg gcttacttat
      901 tgaaaagtga tggatttatc agagggaatg tagaagatgt tctagacctt tattttaaac
      961 aatgctcaat tgagatagat tctgtagatt tagcgagaat tggaataaat cttgcaaatt
     1021 atggagtaga tatagaaaat ggtgagcatt taatgagtga aatggtttct aggattgtca
     1081 agacatttat gatgacttgt ggaatgtatg atgcttctgg ggaatttgct attaaagttg
     1141 gcattcctgc aaaatctgga gttgggggtg gtataatggc ttctgtgccg ggacgcatgg
     1201 ggattggtgt ttatggacct gcgcttgaca aaaaaggtaa cagcgtcgca ggagttaaag
     1261 ttttagagga gttgtcaaat aaactcaaat taaatatttt ttaaaatatt aagaaaggat
     1321 ttttttgaca acaacatata atttaatata taattaaatt gtaaagaaat atgaattaaa
     1381 taaatttttt aggaggggtc aatgaaagag gtattggtat tacgagattt ggagtgcata
     1441 aaggctattg cgcatccacg tagaatagat attctaaaag catttgacaa atcgccattg
     1501 tcagcaaaac aattatctca attattggaa gaacctcatg caaaaataaa ctaccatata
     1561 aaaatgttat acaaagttgg tattttagaa ttagttgagg aaaaaataaa gtcggggatt
     1621 gtggaaaagt actattatcc aagtgcaaaa aatgtggtta tagggaatag gatacttaat
     1681 ttttctttag acaatgggga agaaaaagaa gaattgtaca tatcaaaatt tgaaaatatg
     1741 agtgaagttt tttataaggc agcagaggaa gatgttttag aaaatgaaaa tatagttgat
     1801 tatcatgata tttctcttac tcatgatgaa ttagttgaac ttagtgacac tatgaagtca
     1861 aagatagatg aaattttaaa taaaagacaa cacaatgttg aaggttctaa gtatgatata
     1921 gctatggtta tagttcctac tcttgaggag gaatgtccta gttagagaat taattatgga
     1981 ttaattaaag atataatatt agagattgaa catcaaaatt tacttaataa tcaaaaaata
     2041 gtttttggta tgggtaaaac aatgattttt aatctctatt tttttgtttt tttataccta
     2101 ttgtatttgt aaataatatg tatagtgtaa gatgtataca ctatctaaga agtgcttaga
     2161 taatatacaa agtatgtaag attataatat ttgaaattga ttaaaatggg tataattaaa
     2221 gtgtaaatat taaaattaaa aattaatata aattaaatca atgctagaaa tatgttaaaa
     2281 gaggaatgag attagttggg atagaacatc ccttagagct tacgaatttt tagtgaaaaa
     2341 gtactatcaa ataggaatct ccatcttcat atgttggcta aatgtagtta aggtggagta
     2401 gttcaaaatt aaaattgagt acaagtagta gtacaaataa aaggagatta atatgattaa
     2461 attaggaaca catctttcaa tagcaaaagg atttgctaat gcggctgaga cagctgtata
     2521 tattggtgca aatacatttc aattttttag tagaaatcca agaggaggaa atgctaaaga
     2581 atttgatgaa aaagatataa ttaaatttca agaaattaga aaagaaaata attttgcacc
     2641 actgcttgca catgcaccat atactatgaa tctgggtggt acaaaggatg atgtatacga
     2701 atttgcaaca agggtgatag atgaggatat aagaaggatg gactcattag gaattgaata
     2761 catgtgtttt catcctggaa gtcatgtagg tggtggtgta gattttggca tagatagaat
     2821 agtaaaagga ttgaacaatg ccataaaggg tgatgaaaat ataactatac ttcttgaaac
     2881 tatgtcggga aagggaactg aaataggttt taaatttgag caattaaaaa gtataataga
     2941 caaaattgag cataaagaaa aaataggtgt gtgtcttgat acatgtcata ttttttcagc
     3001 tggttatgat atagtgaatg atttagatgg ggttttggaa gaatttgata aggttatagg
     3061 catagaaaga ttgaaaactg tccatctaaa tgatagtatg atgccatttg gagacaaaaa
     3121 agacagacat gctcctatag gggaaggaaa gataggatta aaagcattaa ttgattttat
     3181 ggaacatcct agtctaaaac atttaccatt tttcttagaa actccttttg atgatgaagg
     3241 tcacaagaga gagcttaaaa tgataaaaga aatactatct agtaaataaa cctttattaa
     3301 aaatatgtga aatactccac cctcaacata attagggtgg agatacaatc atgaaatgtg
     3361 ataatttaaa ttaatggata ggaataacga aaattgtata caaattaact gggtataaag
     3421 tatgattata ttatgaatag actgatataa aatataatta tattatgaat agtatgtaaa
     3481 tacattgcaa agattctcat aatttgttgt ataattaaag gtgacatatt tatattatat
     3541 aaaaaattat taaaagttag gtggggaaag atatgagaaa tttaggcaat actaacatga
     3601 aaataaagag agttggcttt gggggcattc caatacaaag gattactcaa gatgatacta
     3661 acttagttat aaatgagtta gaaaaacaag gaataaactt tattgacagt gcaagagggt
     3721 atacaataag tgaggaagca atcggaattg ccattgaagg aaaaagagat aaatttttct
     3781 tagctactaa aagtatgtcc agagattatg attcaatgaa gagagatgtt gaaataagtt
     3841 taaataattt taaaactgat tttatagatt tatatcaatt ccataatgtt aaagaagagg
     3901 agtatgataa tctttttaaa gataaaatgg cttatagtgc ccttttagag gcaaaggagc
     3961 aaggtaagat aaaacatatt ggtataacta gtcacaattt aaatactata gaaaaagcta
     4021 tagaagatgg aaaatttgac actatacaat tcccttataa tatagttgaa ggacaagcag
     4081 atgaagtgtt taaaaaagct catgaaaaag gaattgggat aatagtaatg aaacctttag
     4141 ctggaggtgc tttagacaat gcaacactgg ctattaaata tatactatct aaagagtaca
     4201 tagatgtagt tataccaggt atggagagtg ttgaacaggt tagacaaaat gtcgctgtac
     4261 ttgaaaatct tgtgctagat gaaaaagata ataaggaaat tgaagaaata agaaattctt
     4321 taggtaaaaa attctgtaga agatgcgaat attgtatgcc ttgtgctgta ggaataaata
     4381 taccattgag ttttttatgt gaaggttatt atactagata tggattaaaa gagtgggcta
     4441 aagaaaaata tgaggttatg gatgttaaac ctactgagtg tattgactgt gggctttgcg
     4501 agagtagatg tccatatgaa cttcctataa gagaaatgct taagactgtt gttgaaaagc
     4561 taggatagat tttatttatt gaaatcggtg tattaaattt actccgattt ctttttatta
     4621 gatgtggtct tatatacaaa tattaaattt acatagttat agctattata taaatttaat
     4681 atttgtttaa tacttattaa atatttactt aatgcttttt aaaaacattt gtttgagtat
     4741 agacataaca caattattat tgttgtatta gtatagtttc aaatactgga gtatcaatat
     4801 aatgtcgaat aacacataaa atctttattt ttcaaaaata ttataggata tatcgtattt
     4861 agatactgga taaagttcaa tttcaaccct agggttatct ttatctataa aatcaaataa
     4921 aagtactggc ttaagttgtt tatcattttt tattattcca cttttttcaa taccatcaca
     4981 aatacttttt ggataattat gtaaatcccc cattctctta tttgggaaga ataatttcaa
     5041 gactgttatt agattttctt cgacagtttc cccttgatac tgttggttta taaaacctgc
     5101 aatcatgttt tcgtatgcaa gatacttaga atattttccc tttgaaggca tccatgcttg
     5161 accatttatg ttatgaagct taaaattaga tttactaata ggtctacctg gaattgttat
     5221 tttaatcaaa tttttcacca tccttataca gtatttatta taatctgtat atgatttaaa
     5281 agcaaattgt tatttaaagt gtttttacag agcttttttg taaaattttc cactttagat
     5341 gtagtaatat aagataagta ttttgcttac aaattttaaa atttgatata tgatggacat
     5401 attcatatta gtctattata agaccttcat aatattaata agtatagttt agtaaagaga
     5461 gtaaaaagaa atccatataa gtcaagtaaa gatttaataa atggcaatgt aaatgggata
     5521 ttaattataa gaaaaaatca atctttttgc ttaactatag aaagaatgtc aaaatataac
     5581 taatgatatt tgaagtttta gagtttatat taaaaggtaa gaaggttaag tgggtaaaaa
     5641 aggttgaata gattaaaaag gctaagtatc taaaagagtt aagtaggtaa aaaagttaaa
     5701 taagtaaatg aaaaaggcta agtatctaaa agagttagta gttaaataag taaatgaaaa
     5761 aggctaagta tataaaggag ttagtagata aaaaaattaa atagataaaa agattaagta
     5821 tctaaaagcg ttagtagata aaaaaattaa ataaataaaa aagactaagt attcaaaaga
     5881 gttagtaggt aaaataatta aatagataaa aagactaatt atttaaagga gttaagtaga
     5941 taaaaaagtt aaataagtaa atgaaaaaga cgaagtatct aaaagagtta gtaaataaaa
     6001 aagatgaagt atctaaaaga gttagtaggt aaaaaactaa atagataaaa agattaagta
     6061 tctaaaagcg ttagtaggta aaaaattaaa taggtaaaaa gacgaagtat ctaaaagatt
     6121 taagtaggta agaaaattga atagctaaaa agattaagta tttaaaagag ttagtagcta
     6181 gaaaaggtta aaaagactaa aaaaattaaa taaatttaaa aagtcaatta gattaaaaag
     6241 gaataatttt atggataata aggaattaaa agcattttgt aatttaatag gattaaactg
     6301 tgtaggagta gcaggggtag ataagtatga taatttagaa aagatactta aggacagaca
     6361 acaaaaaggt tatttaactg gtatggaaga accaataatt gagaacagaa ttaacccaag
     6421 acatataatg aaggatgcta actctataat agtctgtgca tttccatatc atataaatag
     6481 acaagaaaag aataaaaatt caaatttatc aaaatattgt catggaaagg attatcacat
     6541 tgtagtaaag gactttttac aacaaatttg tgattatatt tctaaaacag taacagattt
     6601 taaatacaag ttatttgctg ataatggacc attagttgac aggtatttag cttatttatc
     6661 aggaatagga tattttggaa taaataataa tatcataact gatgaatatg gttcttacgt
     6721 atttataggg tatatagtaa ataattataa atttgaagaa gataaaccac tagaaaaaag
     6781 ttgttttaaa tgtggaaagt gtgtaaaata ttgtccggga aatgctatat tgggaaatta
     6841 tgatatgaac cccaaaaaat gtttatcata tataactcaa aaaaaaggag atttgtcaga
     6901 gaaagaaaaa gttgttttaa agaatggcaa aaaggtattt ggatgcgata tttgtcaaga
     6961 agtatgtcca cataatgttg aaataccaac aactcatatt ttggaattta aagaaaatat
     7021 aatagattat ttgaattatg atgagataca aaatatttca aataaagaat ttaaaaggaa
     7081 atatggagat agagctttta gctggagagg aagaaatata ataaagagaa atatggaaat
     7141 aattttagat aagtatgatg actaataata agagtctgtt tcatatactt taatgtgtat
     7201 ttactttaac acttgttgtg taaaagtcat attatatcat taaagttaaa tatggggagg
     7261 tgggggaata ttgaaatata ataatacatc tgaaataaat ccactagatt atatattggg
     7321 tcaagaaaga gctgtagaag ctatggaatt agggttgaaa ataaataatc cagcgtacaa
     7381 catatatata gctggtgaac ctggaactgg taaaagtact tatgccttaa aggttttaaa
     7441 tgagtatgca tccaaaaaaa atacgcataa agattggtgt tatatatata attttgaaaa
     7501 ccctagagag cctattatag ttgaattaga aaaggggttt ggaagagaac ttaagaaaga
     7561 tatggagaaa ttaattgaaa gtcttttaga agattttaaa aatgcgtttg aaagtgaaaa
     7621 ttttgaaata gaaaaaaata aacttctgga tgaatatgaa atagaaaaag atatgctttt
     7681 gaagcaaatt aaaaaatatg gagaagaaaa aggatttaaa ttaaaacaga gtaaaatggg
     7741 aatagttttt atccctttga aagaagagga agatgaaagt gatgagtctg atgaagagtt
     7801 ttacaaagct aaaagagaac tagaaaatat ggcgatacag gtcgtatata aaattagaaa
     7861 cttagaagaa atagctgaaa aagcagtgtt agagcttgaa gaagagatag ctaaacttgt
     7921 agtagaacct catatcaaga gattgtgtga aaaatatgaa aattatgata agattcaaac
     7981 ttatctggaa aatataaaga aagatataat agaatatatg tatttattct atttagatga
     8041 agaggagtta aaagataagt atgataaaga gcattttatt aaatataaaa ttaacctatt
     8101 tgtagacaat ggaagctcaa agaataaaga atcagcacct gttgtagtag agataaaccc
     8161 aagtccagca aatctatttg gaaaggcaga gtatgattat gcaaatggaa atataaagac
     8221 tgatttcaca aaactattat caggagcatt tcatagggct aatggtggtt atttagtact
     8281 ttatgcagac caattactaa aatatacaat gtcttgggaa attcttaaaa agactttgca
     8341 gactaaaaaa gtaatattgg aaacacaaac agcaattaaa ccagaaaata tgccattgga
     8401 tgttaaatta gtactgattg gaagccacta tatatatgat attttatata gatatgatga
     8461 agattttgaa aaatatttta aagtatttgt agactttgat agtgaaatgg ataaaaatga
     8521 agataatgaa gaaggaatag caagatttat agcataccaa tgtgataaga ataatttaag
     8581 gcattttact aaatcagcag ttgaagaggt tattaaattt agcactagat tatctggtga
     8641 tttagaaaaa ttatcaacta agtttaataa aataatggaa gttgtaatag aaggaagtgc
     8701 ttatgcagag tttagaaatt cggagtatac agagcagtgt gatgttaaaa aagcaataag
     8761 tgaaaagaga aaaagaatca atagagtgga aacacatatg gatgaatcaa ttgaaaatgg
     8821 atttactcta atagaaacag agggaagaaa agtaggagtg ataaatggtt tgtcagtctt
     8881 aagtacagga gaatactctt ttggtagagc ttcaaggatt acagcgacca ctagcccagg
     8941 aagtaaaggc atagtaaata tagaaagaga agtaaatatg agtggctcta tacataacaa
     9001 aggagtactt atattaggtg gctatctttc tgagaatttt gcacaggatt tactattgtc
     9061 actaaatgca tatgtttgtt ttgaacaaaa ttatggtgga atagatggag atagtgcatc
     9121 ttctgcagaa ttatatgtac tattatcatc tcttagtgga gtatcaatta aacaaaatat
     9181 tgctgttaca ggttctataa atcaaaaggg tgatatacaa gtagttggtg gtataagtga
     9241 aaagatagaa ggattttatt ctatatgtaa aactaaaggg ttaaatggtg agcaaggcgt
     9301 cataattcct agaaaaaatc aaagaaatct cgttttatca gatgaagtta atgatgcagt
     9361 gcgagatgga aaatttaaga tatatactgt tgaaagagta gaagaggcta tagagatatt
     9421 gacagatgtt aaatttgaag aaataaagaa attggtaaaa gaaaaattaa tatctttcag
     9481 caagatacaa actgtgtcaa aagaataagt ttattataga atttaatggg tatttatgta
     9541 taataaaaag caagaacttt aataggtttt tgcttttttt gtaaatatat ataaattaaa
     9601 ttcttatttt gtatgtttta tatattgatg tttatttttt tgttgatatg attaaactat
     9661 ttataaatag cgttttataa attggtattt tgaaggggga taattatgaa aaatgtaaat
     9721 ctaaaagaga taaatgaaaa agatgtaatt ttggaagaag agataaataa aaaaggaatg
     9781 gataagaaaa atataattga gaaggaagag ataaataaaa aaggaataga taagaaagat
     9841 ataattgaga aggaagagat aaataaaaaa ggaatagata agaaagatat aattgagaag
     9901 gaagagataa ataaaaaagg aatgaataag aaagatataa ttgagaagga agagataaat
     9961 gaaaaaggaa tagataaaat gattggtaaa aaagatgaga taattgatga gaaagatata
    10021 gagaatttta tggatattga agaagataaa aaaagtatca aagtaagtaa aaaaagtact
    10081 aaaaaaggtg aaacggagaa aaaaacaaag aagagcagtt ctaataaggc tacacaaaaa
    10141 acaagtaaag aaactaaaag taagtctaaa attaaaaatg agtcagcaaa tgaaaaagac
    10201 gtaaataaga ttttagacga gttagaaaaa ttgtatccag atgctaaatg tgagttaaat
    10261 tatgggacag cctttgaact tttaatagca actattttat cagcacaatg tactgatgta
    10321 agagtgaata aagttacttc agagttgttt aaaaagtaca acacagctag agactttgct
    10381 aatttgagca ttgaagaaat tagtaaagag ataaagagtt gtggtcttta caaaagcaag
    10441 tctcaaaaaa taaaggatac atctgaacag ctatgtgaat tatatgatgg tgaagtacct
    10501 gatagcttag aaaagcttat aaaattacct ggtgttggaa gaaaaacggc aggggttgta
    10561 cttagtaacg cttttaatca tccagcaata gctgtagata ctcatgtatt tagagttagc
    10621 aatagaatag gaatagtgga cgagccaaat cctcaaaaaa cagaatttgc acttatggaa
    10681 gctataccaa aagaaagatg gtcacactca catcatgtat taatttttca tggaagaaga
    10741 atgtgtaaag ctagaaatcc agaatgtgca agttgcccaa taaaagaaga ttgtaattat
    10801 tataaagaac ttaatgaaac gaaataactt ttagaaagta agttgtacaa gagggatttt
    10861 ctgatataat aatacttgtg aaaatgttag ataaaaaact attatataca tgaaataaaa
    10921 attattatta acattatttt atataatagt ataatattat tgttattctg tttataaaat
    10981 aaaatttgat aaataaaagc gatacttagg gaggattaaa atgtcattaa atatagtatt
    11041 agtagaacca gaaatacctc aaaatacagg aaatataata agaacctgtg cagcaacagg
    11101 ttcaacttta catttagtaa aaccactggg tttttcattg gatgataaac acctaaaaag
    11161 atgtgggctt gactattggg atatagctga tatacaatat tacgatagct ttgaagaatt
    11221 acaagaaaag tatccaaata gtaaatactt tttttctaca acaaaggcta aacagtcaca
    11281 ttcagatgtg aaatatgaag aaaattgttt tatagtattt gggaaagaaa caaagggatt
    11341 accagaacct ttgttgaaag caaatatgga tacttgtatt agagtgccta tgttggatgt
    11401 tgaaaaagct aggtcattaa atttatcaaa ttcagttgct attgtagtgt acgaggcttt
    11461 aagacaaata ggatacccaa atatgaggta gtttcaacaa gcctaaatga gaaaggagca
    11521 tacttaatga aaaatctaaa gataatctgt gacagtttat cggacgtgcc aaaagatttg
    11581 ttagataaat atgatataga tatagtttca ttaacagtta tactggagga aagagaatac
    11641 aaggatagaa ttgatatatc tggagatgaa ttttataaaa ggcttagaga agaaaatgta
    11701 tatccaaaaa cttctcaagc tacttatgcc caatttaagg aaatttttga gaaatataca
    11761 aatgaggata gagagatact ttatatagca gggtctgcca cagctacagg tacgtatcaa
    11821 agtgctgtaa tggcaaagaa tgatgtagat ggtaaaatat atacatatga ctctaacttg
    11881 ctttgtatgg gaactggttt acttgtaata aaagcaggag aacttgcaag tgaaggaaag
    11941 tctgttgctg aaatattgcc tgttttggat gaattaaaag agaaggcttt tgttatattt
    12001 tcagtagata cattggaata tttacaaaag ggaggtagaa tatcatctac taaggcagca
    12061 ataggaagta tattaaatat aaaaccgatt cttgaaataa gagatggatt agtttcgcaa
    12121 atatctcaag taagaggaaa gaagaatgta atttctaaga tgatggaatt gataaaatca
    12181 aattgtggtg atgatttatc agaccaaatt gtgtatatag gatatagtga tgattttaag
    12241 gaaaaagaac aattatctaa ggttatgcaa gatgaattaa gacctaaaga aattagattt
    12301 ttccaaattg gtgtgtgtat aggtgcacat gctggtcctg gtgttactgg tatcatatgc
    12361 ttaaaaaata aataatatat ttttggatga cattatttat ttttttatta aattaggata
    12421 gaatattaat tgttgagatg ttaaattgga tttattttgg atgaaattaa gtgttgatat
    12481 attagattta gtttgtaggg aagtgtgtaa atagataaga atctattgca cactttttta
    12541 tagtaagatt tattaaaaaa atttcaaaat gtacttgtaa attgtatata aaaaaattat
    12601 aaataggatg taaaagtaaa taggatataa aaatatcgta aatgtatgag gagtgaaatt
    12661 taatgcgttt caaaagaata ttgatattga tgttaactgt agcaatgata gctggaatgt
    12721 tggttgggtg tgctaataac ccaagtaata atcctagctc tagcaaagat aataaaagtg
    12781 atataagcga taaaagtaat tcaaaaaaat ctgtatcaat gatactagat attgaaggaa
    12841 caaataatga agctatgaat aattcagctt tattagcatt aaacaatgct caaaagaaac
    12901 ttaatataga tacaaacaaa gtggaatcag atgattcctc aactttttct aatagtatag
    12961 atattttatg taatgacaat tacgatttaa ttatagctgt aggagctaga tttgcaaaac
    13021 cattggagat ggtagccaag aaatatccaa agcaacaatt tgcaattata gactatgagt
    13081 atgacaagca accttctaat ataacttcaa tttcttatga agataataaa tctggatacc
    13141 tagctggatt aattgctgga aaaatgactg aaagcgataa ggttggtttt ataggaggaa
    13201 caaagagttc tagtagagat aaatttgaat caggttttag agagggtgtt aaattttcta
    13261 atagtagtat aaaggatatt tcagttgagt atgcagatgt ttttaaagat agcaaatcag
    13321 tagaatctat agctaaaaaa atgatggata atggagtgga tattattttt tctactactg
    13381 aagatgattc aaaagctgta atagatgcag taagggcaaa aaataaaaaa gttatagcaa
    13441 cgaataaaga ccaacatgag ttagctccag aaaatgttat aagttctatt gtaaaagatt
    13501 ttgagaatcc tacttataat ctgatacaaa gttttgtaaa aggaaattat aaaggcggaa
    13561 aggttataga aaacacagtt aagtctggaa gtactggact tgcccaaaat agttctcaaa
    13621 atattcctcc agatgtgtta gaatatgtta ataagaacaa taaataatct actataatat
    13681 ccaagaatac ttatgagttt agttatgagg tgaattggat gttataatag acatatatag
    13741 aaagacatat gcagaggtag gtcagaccta tctaaatcag ggagtgaatt ttgtgaaaat
    13801 aaaaaggata ttgatggctt gctttgtagt agtttttaca atttttatat tagctatatg
    13861 taattttgat tttgggtttg ataaagtaaa taagaataat tctaatatag atagtgctaa
    13921 atcgaaacag agtgagtcta aaagtttgct aacttcagca gaaaaggata aatcaaatag
    13981 taaaagtaag aataaagaag atgacacaga agatgattca tatgtaagaa aaactattgg
    14041 agtattgtta gataccgaaa acttaaataa cgaatcatat aataacaatg tactatcagc
    14101 cttaaaaagg gctgacgaag attttgatgt taggataaaa cttgaaaaac caaagacttc
    14161 taaaagtttt aaagagagta tagaaaaact gcataagtat gaaccagatt ttataatagc
    14221 agttggagcc agatttgcag agccaattaa agaagctgct actaaatatc ccaaacagca
    14281 atttgcaata gttgactata cttataaagt tcagccagct aatgtaattt ctgtatcatt
    14341 tcaagataat gttactggat atttagcagg acttattgca ggtaaaatga ctgatactgg
    14401 gaaagtcggt tttataggag gagttgaggg ttctagtaga gataagtatg aatctggatt
    14461 tagaaagggg cttaaaactt caaataaaga tgctgagtta ctagttaagt attcaaatat
    14521 atttgaaaac cctgaatcag ctagaactat tgcaaaggaa atgagagatt ctgatagaga
    14581 tgttgtattt tcagctacag aagatgatag taaaagagct attcaggtag caagagaaaa
    14641 tggtggtaaa gttatagcta ttaataaaga ccaatacgat atggctccag ataatgtaat
    14701 aagttcaatt ataaaagatg tagaacaacc tgtatatgaa ctgataaaga acgttgcaaa
    14761 agatggtttt aagggaagta aagtaatgga atttaaaata aaagatgggg aacttggctt
    14821 gacttcaaag agcagtagaa atataccacc agatgttttg gaatacataa aaaaagagta
    14881 taacaaagtg aaagatacat attaattatt gatgtagaag attataaaaa tatattgctt
    14941 aagtttagat tagcaataaa aagatatatt tattaaattt gtgctaactg tacaaaatat
    15001 attgattaag tttagattag tgataaaaag atatatttat taaatttgtg ttaacagtat
    15061 ggaatatact gcttaagttt agattggtga taaaaggata tatttattaa atttagtcta
    15121 actgtacaaa atatattgat tgagtttaga ttagcaataa aaagatatat ttattaaatt
    15181 tgtgctaact gtacggaata tactgcttaa gtttagatta gtgataaaaa gatatattta
    15241 ttaaatttga accaactgta caaagctata ctaattaagc ttagaataac tatataaaat
    15301 tataacaata agtgtgtggg cagaaaaatt ctgctacaca ctttatttat attcacataa
    15361 aaaatttatc aaaaaaaaga aaaaggagta aaaaatggaa tttacaagag aaccatttat
    15421 actagatgac gatttgtgtc tagctttggt ggacaaaaga attagttatc taatggaaaa
    15481 tgaattaaaa ttaagaaata tagagataat aaaaacaata gaatgtaaag aattatatga
    15541 aagcataaaa tatcacccgg atatatgtat ttgtaattta ggaaaaggag atataattgt
    15601 tgcgccgaat gtatataatc aatacaaaga gttattaagt agatataaat ttaatataat
    15661 taagggagaa gctaatttat taaatagata cccttatgat ataccttaca atattgcgat
    15721 agttggtgac tatgctatac acaattttaa gtatactgac aaagcaatat tagattatat
    15781 tgaaaataat aaacttaaaa agataaatat agagcagggc tattctaaat gctcaatctg
    15841 tatagtagat agaacgtcta taataacatc agataaaggt atttggaaaa gtatgaagaa
    15901 tacagaaata gagtgtttat tgatagaaaa aggtcacata aatttatttg agatgaatta
    15961 tggatttata ggtggatgta cagggttgat ttctaaggat aaactagctt tttgtggaga
    16021 tgttaaaaaa catcctgact atgaacgtat aaaaagtttt gtagaaagta aaaacaagga
    16081 gatagttaca cttagctgtg aaaatttact agatataggt tctatagttc ctctcatgac
    16141 aagaaaggag cgataacttt ggttgagaag gataatattt cttccatgaa atttttgagg
    16201 aataataatg taaacgatag attaatatat acgaatgaca ttgtagaagt agtgatagat
    16261 tctgcaaagc aagaagtgtt aaataaaaaa atacttaatg gtactaaagc tgaacaaaga
    16321 gctttaaata ttgatgagat atcaacggaa ataactagta tgataataag tataatgaaa
    16381 gagaaacttt tgaaagaata cattctaaaa tgctatgggc gtacatatcc tgaagataaa
    16441 aataatatat acatatattc actgaacata tttgctaaaa aagagatttt tatgagagaa
    16501 acagtattta ctagggtgag aaattatata gaagaaaatg attttataaa tgtagaaggt
    16561 tttattagat ttagaatgag agaatttatg aaatatattt ctgctatagg tgatattgca
    16621 gttgaggagt atttaataaa gaaagaccaa gatgaattta taagagtttt aaaatacttt
    16681 atagatactc aagaagaaaa gattgatttg cttaaagttc atataatgga agataataca
    16741 tttgttttat acgataaaaa tggaaataag atagacagta tagatgatga agaaattata
    16801 aatatggtaa taagagaaaa tttaaattat gaggactttt taataagtac cttattaact
    16861 ctatgtccca aaaaaataga aatattggat ttgttaaata ataattgctc aaaggaaatt
    16921 atagatactg tagaagcaat ttttgaaaat aaagttagta taattatgga aaactagaat
    16981 ctaagcatat aaaagctatc catataaaat taaaaaaatt ctttttaaat cgacacaaaa
    17041 gacttataat tgtatataaa gctatagtat aactagagtt attaagtata aagaatatct
    17101 acttgattaa tgcaatatag ctggcaaata tacaattgat aaggagtaat tttataatgg
    17161 ataatttaat taggatactg atattagctg ttattggtgg atttataggt tatgttacga
    17221 atgtagttgc tataagactg atatttagac caatagagcc tattaaaata cctattttaa
    17281 atatagaaat agtaggtctt ataccaaaga gaagagcaga aatagcagca aatgtaggag
    17341 aaataattca agaagaattt ctttctatgg acgaaatact tgcaaatatc attactgacg
    17401 aagataagga agaagttgta agatacataa aagcaagggt aaaaattata atacatgaga
    17461 aagtttcatt tatcccgagt ggtataaaaa acatgataca agattatcta ggagagataa
    17521 ttgagtctga agttaagcaa agtatagatg aattgagtaa aaatataata aataaagcaa
    17581 atgaacgtat agatatacaa aaaatggtcg aagataagat taatgaacta gatttatatg
    17641 aattggaaga gataataata agaatagcta agaaagaatt aaaacatata gagtttttgg
    17701 ggcttgtctt agggttttta ataggtatag ctcaagggtt aattactatg tttatttaaa
    17761 tatatgagaa gtagcattta tttaagtact aattatattt acctatttga gtgaattact
    17821 tattaataac atttagatat taattgtatt tacctatttg agt
//
LOCUS       DAYAPA010000084        17686 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.084, whole genome shotgun sequence.
ACCESSION   DAYAPA010000084 DAYAPA010000000
VERSION     DAYAPA010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17686
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.084"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     misc_binding    211..442
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            550..2031
                     /gene="thrC"
                     /locus_tag="ACGI59_002592"
     CDS             550..2031
                     /gene="thrC"
                     /locus_tag="ACGI59_002592"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HFG9740374.1"
                     /translation="MYYSSTRGTEEKVTASQAIIKGISNDGGLYVPSEFPNVKNELIN
                     LVNLTYSQIAFFVLSKFLCDFTEDEIKNCIENAYDEKFDCSSIAPLNKVNDTYFLELY
                     HGPTLAFKDMALTIMPHLLKTSIKKDNLEKDVVILTATSGDTGKAALEGFKDIDKIKI
                     IVFFPEDGVSPVQKLQMKTQTGKNTYVVGIKGNFDDAQSGVKKIFSDKEFNQKLLDNN
                     YILSSANSINIGRLLPQVAYYFYSYMKLVSNSDINLNDKINFVVPTGNFGNILAGYYA
                     KQMGLPINKLICASNDNNVLYDFFKTGVYDKNRTLKLTTSPSMDILISSNLERLLFEI
                     SNRNTDIVNKLLSDLSDKGVYKISDDMEKNLASFYGEYSNEEEVKSTINNVFKNYNYL
                     IDTHTAVAYNCYEKYKKETSDNTKTVIVSTASPFKFSEDVLKSIDCDFKDLDDFAIID
                     RLSSISKIDIPKPIKNLKNAEILHKDIYEKDELKLAIKKFLKV"
     gene            2041..2934
                     /gene="thrB"
                     /locus_tag="ACGI59_002593"
     CDS             2041..2934
                     /gene="thrB"
                     /locus_tag="ACGI59_002593"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HFG9740375.1"
                     /translation="MLEIIVPATSANIGPGFDCLGIALNIYNKFYVEEIESGLEIEGC
                     EDAYKNENNLVYTSMKYFFDRVKPEKIPAGIKIKIQSEVPICRGLGSSASCIVAGVIA
                     ANALSGANLDKNQLLNIASEIEGHPDNVAPAILGNMIVSVTDNENIHYDIIKIPEELK
                     FCAMIPNFKLSTEKARGVLPKEIPYSDGVFNVSRVALLISALLNKNFDLLKVACQDKL
                     HQDYRGTLIENYNDIVEKSEQLNSIGVFLSGAGPTIMSLIKENDDSFVDNMKNYLQKL
                     KSDWEIKELCCDSNGAVLNII"
     gene            3064..4119
                     /locus_tag="ACGI59_002594"
     CDS             3064..4119
                     /locus_tag="ACGI59_002594"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent flavin oxidoreductase"
                     /protein_id="HFG9740376.1"
                     /translation="MKELIIRDLVAKVPIIQGGMGVGISRSSLAGAVAKLGGVGVISG
                     VQIGYDEEDFETNTINANLRAIKKHISKAKEISNGGIIGINFMVAMKEYETYVKEAVK
                     AGVDLIISGAGLPNKLPSLVKGSNVKIAPIVSTAKAANVILKMWDRKEKTTADLIVVE
                     GPKAGGHLGYSNEELDNIDSIDYDKEFVEILKVANTYGEKFGRNIPVVAAGGITSSSD
                     VKKYIDMGASGVQVGTRFVATYECDAHENFKMAYINASEEDVHIVKSPVGLPGRAIRN
                     KFIEEVKINRPEIKKCYNCLIPCNPKETPYCISQALINAVKGDVENSLLFCGNDAYKI
                     DKLSTVEDVINELISEL"
     gene            complement(4274..4786)
                     /locus_tag="ACGI59_002595"
     CDS             complement(4274..4786)
                     /locus_tag="ACGI59_002595"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFG9740377.1"
                     /translation="MSDFKEILAEEIKNNPFGLIGKDWTLITAENEGKVNTMTASWGG
                     LGVMWGKDVAFVVIRPQRYTKEFIDNTDKFSLTFFDEDFRKELSYCGKVSGREEDKIS
                     QIGFNIEHLNDTPYFKESKMAIICKKMYSQKLEPQCFIAEGIDGRWYPQKDYHTLYIA
                     EITNVLVKED"
     gene            5306..6241
                     /locus_tag="ACGI59_002596"
     CDS             5306..6241
                     /locus_tag="ACGI59_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFG9740378.1"
                     /translation="MIRYYKTIDSKLEKLSFFEDGCWINLVEPNHSEINEISNLLNID
                     VESIESALDEEERSRIDVEDNHTLILIDIPVDESDSNSSHYTTIPLGIILTEEAIVTV
                     CDAQTKILNDFIVGHIKDFFTFKKTRFLLQILHKNAAYYLHYLRKINKMTIIIEREIY
                     KSMKNKELVQLLELEKSLVYFSTSLKSNELVLNKMVRTAGIKKYPDDEDLLEDVIVEN
                     RQALDMAKIYGDILSRIMDAFSAIISNNQNNVMQILTVVTLIFSIPTIISGFFGMNVI
                     NMPFSNNPNGFWIILLITSIICIVITFFMSRNKLL"
     gene            complement(6333..8204)
                     /locus_tag="ACGI59_002597"
     CDS             complement(6333..8204)
                     /locus_tag="ACGI59_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFG9740379.1"
                     /translation="MKNTKFSIKKIKNISNDPKVVHTKGALLEKMGKMESAIELYKSA
                     ALDNYVKSQYTLGNIYESKKQFKEAEKWYSMAYKSGSEDAAFDLGNMYYKLDGYEYAI
                     YWYEKIANLGYLQAQNNLGVCHFKMKDFVRCEKWLKTAADKNLGKACFNLGVLYTFLE
                     KDDEAYEYYKKGSVLLDDDAKYNLAILNGKKKDNKSTVSLYKQLYKSGHMKGCFNLGM
                     IMEINDNLEDAEKYYKKSADRDDVKSEYRLAYVYDRKEELGDAIYYYEKAIEKNHTLS
                     KYRLANLYNRENEIEKAKTYYKMAAEDDITEAKNNLAGIYFEEKDYENAIKYYEDAIA
                     VGCKSSLENLGDLYYQNQDIEKAISYYSRIPNNVSCQIKLGNIYEDLNNIEEAISWYK
                     KASENGDTRSSYRLGCIYESLGNTKNARKYFEMASSKNHMNARIHLGRIYFREGKLEE
                     SKMMFDTPANENNVYAQHMVGLIYDMFYKDYVNSKFWYEKARAQGCVESIYNLGQIYL
                     KLNDDAEAEKYYKEGIKYGSKKCEYMLAGLYYKKSLDMYISLANEEYDNCEEIVEGFP
                     NLNIDFDEVLIPPFKLQEEVIDEEEYVPIYILNIKESLDSMLEGLETEMIIDEEHDS"
     gene            8394..9320
                     /locus_tag="ACGI59_002598"
     CDS             8394..9320
                     /locus_tag="ACGI59_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HFG9740380.1"
                     /translation="MNFSNIFIQVAVLFIIILVGYFVRKFNLLDDHCTSKLSTLTMTV
                     FLPSMIISSMQINFDSKMIQKILLLLFTSLIMYIVSIIIAFLLKYILKCNDKKDLGIY
                     QYIVVFSNVAFMGYPVIEAVLGHEAIFYTAIFNLPFNLFSFTLGIYLLSKGSTSRGFS
                     IKSLISPATIAIVIGLFLFVTGLRLPQFINEPLEMLGSITTPISMIIIGSLLANSSAL
                     DCFVNKKLYIVTFIRLLVLPVIVYFILKGWINDKMILAIPVVISSMPAAANTAIMANQ
                     YDSNITLASQCVFFTTLFSVISIPFISILLLS"
     gene            9459..9605
                     /locus_tag="ACGI59_002599"
     CDS             9459..9605
                     /locus_tag="ACGI59_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9740381.1"
                     /translation="MDMDLLFKIVFAIVAIGVSVKIIKTVASLAFKIALILLIILFVY
                     RMFL"
     gene            complement(9710..10234)
                     /locus_tag="ACGI59_002600"
     CDS             complement(9710..10234)
                     /locus_tag="ACGI59_002600"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFG9740382.1"
                     /translation="MSKPIDITIRPIRREDAQAFNEIRRTYSVMRNTLALMSDRVDKT
                     EAMLASLGENDYMFVAETNNNGEKDVIGFAGLHVNSSPRLRHSAEIGISVAENYQGRG
                     VGKKLMEQLLDIADNWIMLTRVGLEVIVDNEKGLNLYKKLGFEIEGTKKYAVIRDGKF
                     EDVYIMGRYNKNLI"
     gene            complement(10287..10511)
                     /locus_tag="ACGI59_002601"
     CDS             complement(10287..10511)
                     /locus_tag="ACGI59_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9740383.1"
                     /translation="MIIKLTKDNFNFRFKQYEGMFFHAEVVEGMESIRYKITKDKEGT
                     ELKEFGSTHIKDNMIYVPQVEAYIDLNSIK"
     gene            10708..11832
                     /locus_tag="ACGI59_002602"
     CDS             10708..11832
                     /locus_tag="ACGI59_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3810 domain-containing protein"
                     /protein_id="HFG9740384.1"
                     /translation="MSKKTKYFSLILFPIALLLNFIASKVPNIVEKYYSQFIDKIIVQ
                     ILSKVSGIFPFSLYEITMYIIVISTFLFLCCTLSTIFNKKKKLNIYLKNSILNILSIV
                     SIVYFLFVVLWGINYNRIPLETTLINNYNLKNNTSIQSKQYSVKELTKLYKFLVIKAN
                     ETRKLTLQDKNGIVKSNTDYNGVINRAQLGYDNILDILPGVSDSYSKPKYIISSNLMC
                     YTGITGIYFPFTGEANVNIAIPDLYIPCTVEHEMAHQRGFASEDEANFIAYLTSIKHP
                     NIDFNYSGYILALNYTASALSKVDYNAYVDISAGISDSVRRDLKNESEFWQKYEGKIN
                     EISNEFNNSYLKANGVTEGTQSYGKMVDLLLTYYELYPYN"
     gene            complement(12306..13130)
                     /locus_tag="ACGI59_002603"
     CDS             complement(12306..13130)
                     /locus_tag="ACGI59_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5780 domain-containing protein"
                     /protein_id="HFG9740385.1"
                     /translation="MRYKKISSLPILVVALLITGTTTTFSLSTTNSEKTNVYLANTVS
                     TVTKKVSDIQSSKGLVYNENIPLFINNSKIQYDDTPYHWPSNVISLTNSSEKAIMDYE
                     ITCLAYDKNGKPLELYWDAQNVAADGEVGSVGFSPAGVDYGIVTGISPVSPKSYSHTY
                     RKMQQSPPQDIISMFEKQQGKAWVENWLKEWKQMEKEYAKQNAIAPGKNQNDAFLLFD
                     KWKQSTGEHGVKYIISCVKQVTFNDGSVWKNSAYENWLKSFQGKEVSNSVLENYYK"
     gene            complement(13478..14533)
                     /gene="ispG"
                     /locus_tag="ACGI59_002604"
                     /gene_synonym="gcpE"
     CDS             complement(13478..14533)
                     /gene="ispG"
                     /locus_tag="ACGI59_002604"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HFG9740386.1"
                     /translation="MSYERRKTREVSVGAVKIGGENPISIQSMTNTDTRDADATIAQI
                     KRLEEAGCDIVRVAVPDIKAAKNIAKIKSSVNIPIIADIHFDYKLALEAIEQGVDGIR
                     INPGNIGSIERVKMVVEKCKERNLKIRIGVNGGSLEKELLKKYGSATAEALVESAMGH
                     IKILEDLDFHNIVISLKSSDIYKTVDAYELISKKVDYPLHIGITESGSVHKGTIKSSI
                     GVGALLLKGIGDTVRISLTGDPVEEVIVGKQILRSLGLLNDKIKVISCPTCGRCNIDL
                     ISVVNEVEEKIGSMEKNITVAIMGCAVNGPGEAREADIGIAGGKGEGLLFKKGEIVRM
                     IDGNKLVDELLEEIEKL"
     gene            complement(14716..15720)
                     /gene="rseP"
                     /locus_tag="ACGI59_002605"
     CDS             complement(14716..15720)
                     /gene="rseP"
                     /locus_tag="ACGI59_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HFG9740387.1"
                     /translation="MTIIAALILFSIIVLIHELGHFIFAKRSGIKVNEFSIGMGPKIY
                     SVKKDTEYSIRALPIGGYVSMEGEDEEQISPNSFGNKSILQRFSTIVAGPIFNIILAA
                     ILLVPVFLYIGSPTTKLGKIMPDTPAQAVGLQVGDKINKINGNSVKTWDEVANIINTS
                     SGGELKLSITRDGSDKVVNVTPKNNNGKYEIGIQPQREKDFLGSIVNACKTTVDMTKQ
                     MLTFLGQMITGRVPGGIGNAVAGPVGVIGMVSDAARTGIINVVYLAAVISLNLGIVNL
                     LPIPALDGWRILMLLLEAVRGGKKLDPNKEGMINVVGFGALMLFMLFITYKDILRLFQ
                     "
     gene            complement(15733..16887)
                     /locus_tag="ACGI59_002606"
     CDS             complement(15733..16887)
                     /locus_tag="ACGI59_002606"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HFG9740388.1"
                     /translation="MKKISILGSTGSIGKQTLDVVREHRDKFEIVAISANSNIELLLE
                     QIVEFKPKYVTVFEENKALKLKEMLPKNIEIEVLAGMEGLKIISSLDEVDVLLTAVVG
                     MIGLVPTLCAIKKGIDIALANKETLVTAGELVMKEAEKYNVNILPVDSEHSAIFQCLN
                     GENKKNIEKIILTASGGPFRGKKKGELANITKNEALKHPNWSMGRKISIDSSTLMNKG
                     LEVIEARWLFGVEQENIDVVVHPQSIIHSMVQYTDSSIIAQLGCPDMRLPIQYALTYP
                     DRMESSFERMNFSKFSTLTFEEPDLETFPCLKLAYECLKMGGTYSSVLNSANEVLVSE
                     FLEDKIGFYDIPYYIEKTLEVHSSISEPTLEEILETDRWSRAYVANLIKK"
     gene            complement(17051..17254)
                     /locus_tag="ACGI59_002607"
     CDS             complement(17051..17254)
                     /locus_tag="ACGI59_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFG9740389.1"
                     /translation="MISYAPLWKLLIDRKIKKMEFVNISGISISVLGRLGNDKSVSMD
                     TMEKICLALDCKIEDVVEIKKGI"
BASE COUNT         6114 a         2730 c         2089 g         6753 t
ORIGIN      
        1 gttatctttt aattactttc attaaaaaca gcttcatcac tatgaaaatc taggttttta
       61 aacttttaaa aaagttaaaa aaatattttt ctaatactca atttgattac aattttttta
      121 caatttaaaa aatatttttt ccaaattttt atattaatac ttgactttgt ctgttttata
      181 aactagaata attaataata ttaagaatat tgaaaggcat tgattaggaa gagtaaatct
      241 gaatcgatta ccagagaagg aaactactag ctgaaatgtt tccctaatta gaaggattga
      301 aaactaccta ggagcttcta tccgaagtta tcttaatgat tttagtagga atagacgttt
      361 ttattacttg cgttaaagta tcaagtggat tttttaaaat caatttgggt ggaaccgcgg
      421 gaaacagatt ctcgtccctt ttttagggat gagggtcttt ttttattacc taaattcagt
      481 ttttaaaata ttctgtatct aatttaatta gctaattaaa aatttaaaat atatggagga
      541 ttataaaaaa tgtattattc aagtacgaga ggcaccgaag aaaaagtaac tgcatcacaa
      601 gcaataataa aaggaatatc aaatgatggt ggtctttatg ttccaagtga atttcccaat
      661 gtaaaaaatg aacttataaa tttagtaaat ttaacgtatt ctcagatagc attttttgta
      721 cttagtaaat ttttatgtga ttttactgaa gatgaaatta aaaattgtat agaaaatgca
      781 tatgatgaaa agtttgattg tagtagtata gctccattaa ataaagtaaa tgatacttat
      841 ttcttagaac tatatcacgg tccaacttta gcatttaaag atatggcact tactataatg
      901 cctcatcttc ttaaaacatc tataaagaaa gataatttag agaaagatgt tgtgattctt
      961 actgctacat ctggagatac aggtaaagct gcacttgagg gatttaaaga tatagataaa
     1021 ataaagataa ttgtattctt cccagaagat ggtgtaagcc cagtacaaaa acttcaaatg
     1081 aaaactcaaa ctggaaaaaa tacatatgta gttggtataa aaggaaattt tgatgatgca
     1141 caatcaggtg taaagaagat tttttctgat aaagaattta atcaaaaact attggataat
     1201 aattatatat tatcttctgc aaattcaatt aatataggaa gacttcttcc acaagttgca
     1261 tactattttt attcatatat gaaattagtt agcaatagtg atataaattt aaatgataaa
     1321 ataaattttg ttgtcccaac tggtaatttt ggtaatattc tagctggata ttatgctaaa
     1381 caaatgggtc ttccaataaa caaattaata tgtgcatcaa atgataataa tgttctttat
     1441 gatttcttta aaactggtgt atatgataaa aatagaacat tgaaattaac tacttctcct
     1501 tctatggata tattaatttc aagtaattta gaacgattac tgtttgaaat atcaaataga
     1561 aatactgata ttgtaaataa gcttttatca gaccttagtg ataaaggagt ctacaaaata
     1621 agtgatgata tggaaaaaaa tttagcctca ttctatggag aatattcaaa tgaagaagaa
     1681 gttaaaagca cgataaacaa tgtgtttaaa aattataatt atttgataga tactcatact
     1741 gctgttgcat acaattgtta tgaaaaatat aaaaaagaaa cttctgataa cacaaaaact
     1801 gttattgtaa gtactgcaag tccattcaag ttttctgaag atgttctaaa gtctatagat
     1861 tgtgatttta aagaccttga tgactttgct ataattgata gattatcaag tatttctaaa
     1921 atagatattc caaaacctat taaaaatctt aaaaatgcag aaatactaca taaagatatt
     1981 tatgaaaaag atgaattaaa actagctata aaaaaattct taaaggtata ggtgattatt
     2041 atgttagaga ttatagttcc tgcaacaagt gctaatatag gtccaggatt tgattgtctt
     2101 ggaattgcgt taaatatata caataaattt tatgttgaag aaattgaaag tggtcttgaa
     2161 atagaaggtt gtgaagatgc ttacaaaaac gaaaacaact tagtttatac atctatgaag
     2221 tacttttttg atagagttaa accagaaaaa atacctgctg gtatcaaaat aaaaatccaa
     2281 agtgaagtac ctatatgtag aggtcttggc agtagtgcct cttgtatagt agctggagtt
     2341 atagctgcaa atgctctatc aggtgctaat ttagataaaa atcaattact aaatatagct
     2401 tcagaaatag aaggtcaccc agataatgtt gctcctgcta ttttaggaaa tatgattgta
     2461 tctgttactg ataatgagaa tatacattat gatattataa aaattcctga agaacttaaa
     2521 ttttgtgcaa tgatacctaa ttttaagtta tcaacagaaa aagctagagg tgttttacca
     2581 aaggaaatac catattctga tggggtattc aatgttagta gagttgcttt actaatatct
     2641 gccttactaa acaaaaactt tgacctactt aaagtagctt gtcaagacaa attgcatcaa
     2701 gattatagag gaactctaat tgagaactac aatgatattg ttgaaaaatc tgaacaatta
     2761 aatagtattg gagtattttt aagtggagct ggacctacta taatgtcttt aataaaagaa
     2821 aatgatgata gttttgtgga taatatgaaa aattacctac aaaaactaaa atctgattgg
     2881 gaaataaaag agttatgttg cgattctaat ggagctgttt taaatataat ataattatcg
     2941 tcttctatac aaaatataac tactctttta ttataatttg tagtataata attaggacta
     3001 ggtaataata ttaagtatta taatctagtt ctttaaacat gtttacattg aggagggatt
     3061 atcataaaag agttaataat tagagattta gttgcaaaag ttcctataat tcaaggtgga
     3121 atgggcgttg gtatatctag gtcatcacta gctggtgcag ttgctaagct tggtggtgtt
     3181 ggtgtaattt ctggagttca aattggatat gatgaggaag attttgaaac taatactata
     3241 aatgcaaatt taagagctat aaaaaaacat atttctaagg ccaaagaaat ctcaaatggt
     3301 ggtattattg gtattaattt tatggtagct atgaaagaat atgaaacata cgtaaaggaa
     3361 gctgttaaag ctggtgtaga ccttataata tctggagctg gattacctaa taagctacca
     3421 tcattagtaa agggaagtaa tgtaaaaata gcacccatag tttcaactgc aaaagctgct
     3481 aatgtaattt taaaaatgtg ggatagaaaa gagaaaacta ctgctgactt aatagttgtt
     3541 gaaggtccaa aagctggtgg tcaccttgga tactctaacg aggaacttga taacatagat
     3601 tctatagact atgataagga atttgttgaa attttaaagg ttgcaaatac ttatggagaa
     3661 aaatttggta gaaacatacc agtagttgct gctggtggta taacttctag tagtgatgtt
     3721 aaaaaatata tagatatggg tgcaagtggt gtacaagttg gtacaagatt tgttgctact
     3781 tacgaatgtg atgcccatga gaactttaaa atggcataca taaatgctag tgaagaagat
     3841 gttcatatag ttaaaagtcc tgtaggtctt cctggacgcg ccataagaaa caaatttata
     3901 gaagaagtta aaataaatcg tccagaaata aagaagtgct ataattgttt gattccttgt
     3961 aatccaaaag aaactcctta ctgtatttct caagctctaa tcaatgcagt taaaggagat
     4021 gttgaaaatt cacttttatt ttgtggaaat gatgcctata aaattgataa attaagtact
     4081 gttgaagatg ttatcaatga attgatttct gaattataat ttaaagaaaa ctaaattaaa
     4141 attttatcaa gctaaggagt ttaaatacgt tatttattaa tctttataaa catatttatg
     4201 tccttagctt ttttgtgtca atttcctttt atggaaattt taatttaatt agagatagta
     4261 ttaaccttta taactaatct tcttttacaa gtacattggt tatttcagct atatataaag
     4321 tatgataatc tttttgtgga taccatcttc catctatacc ttcagcaata aaacattgag
     4381 gttcaagttt ctgactatac atttttttgc atataatagc cattttagat tctttaaaat
     4441 aaggtgtatc atttaagtgt tctatattaa atccaatctg tgatatctta tcctcttctc
     4501 taccagatac ctttccacaa tatgaaagct ctttacgaaa atcttcatca aaaaatgtaa
     4561 gagaaaattt atctgtatta tctataaatt ccttagtata acgctgtggt cttattacta
     4621 caaaagcaac atcttttccc cacataactc caagccctcc ccagctagca gtcattgtat
     4681 tcactttacc ttcattttct gctgttataa gtgtccaatc tttacctatc aatccaaatg
     4741 gattattttt aatctcttct gccaaaatct ctttaaaatc actcatatct ctaataacct
     4801 ctcattctct tatatttata ttccttttat tttcgtgata aattataaat gaagtctagt
     4861 tttgaaaata aaatactttt ttaaatatat tattgcaaat aagtaaatat atatatactc
     4921 tcttattttt taatatattt aataatttca ctcagaatat atatattctt tactcaaatt
     4981 aaatataact tatatacttt atgtgtaaat tatggtaaaa atattattta atcaaatcaa
     5041 aacttgattt atatacctgg aaaaggtatt atattaaaga gtgcttaaaa tacgcatttc
     5101 ataaatagct atatataaaa taataaataa ggagagtgat gggcaatgga tactagtccg
     5161 ttgcggagag aattttttac attttcatca cttaataata tctaaaatca aaactaatac
     5221 atatattcag ttatataaat tagttaatta atagatacta ttaaatgatg agatttatat
     5281 aaaactgaat aaaggatgtg ttgccatgat aagatattac aaaactatag attcaaaatt
     5341 agagaaactt agtttttttg aagatggctg ttggataaat cttgttgaac ctaatcatag
     5401 tgaaatcaac gaaatttcta atttattaaa tatagatgta gaaagcatag aatctgcact
     5461 agatgaagaa gaacgctcaa gaatagatgt tgaagacaat catactctta tacttataga
     5521 tataccagta gacgaaagtg actctaattc ttctcattat actacaatac ccttaggtat
     5581 tatactaact gaagaagcta ttgtaacagt ttgtgatgca caaactaaga ttttaaatga
     5641 ctttatagtt ggtcatatta aagatttttt tacgtttaaa aagactcgct ttttgcttca
     5701 aattcttcac aaaaatgcag cttattactt gcattactta agaaaaataa ataaaatgac
     5761 aatcataata gagcgagaaa tttacaaatc aatgaaaaac aaagaattgg ttcaattatt
     5821 ggaattagaa aaatctttgg tatacttctc tacctcacta aaatctaatg aacttgtttt
     5881 aaacaaaatg gttagaactg ctggtataaa gaagtatcca gatgatgaag acctattaga
     5941 agatgttata gttgaaaata gacaggctct tgatatggct aaaatatatg gagatatatt
     6001 gagtagaatt atggatgctt tcagtgcaat tataagtaat aatcagaata atgttatgca
     6061 aattttaaca gtagttacac taattttctc aatacctaca ataatatcag gattctttgg
     6121 tatgaatgtc atcaacatgc cattttctaa taacccaaac ggattttgga taatacttct
     6181 tattacctct ataatatgta tagtgattac atttttcatg tcaagaaata agctattata
     6241 aaatattaat aaaaaggcca ttagaattaa ttgatttaca tttatcctaa tggcttttat
     6301 atatttaaat ctatataatc ttataatact ttttaactat catgttcttc atctataatc
     6361 atttcagttt caagcccttc tagcattgaa tctaaacttt cttttatatt tagtatataa
     6421 attggtacat actcttcttc atctattacc tcttcttgca acttaaatgg aggaattaat
     6481 acttcatcaa agtctatatt taaatttgga aagccttcta ctatctcttc acaattatcg
     6541 tactcttcat ttgctaaaga aatgtacata tctaggctct ttttataata aagacctgca
     6601 agcatatact cacacttctt acttccatat ttaatacctt ctttataata tttctcagct
     6661 tctgcgtcat catttaattt taaataaatt tgacctaaat tatatattga ttcaacacaa
     6721 ccttgtgctc tagccttttc ataccaaaat ttagaattta cataatcttt atagaacata
     6781 tcataaatta atcctaccat atgttgagca tacacattat tttcattggc aggtgtatca
     6841 aacatcattt tagactcttc caatttacct tctctaaaat aaattctacc tagatgtata
     6901 cgagcattca tatggttttt gctacttgcc atttcaaaat actttctagc attttttgta
     6961 tttcctaagc tctcatatat acaccccaat ctataactag acctcgtatc tccattttca
     7021 gatgctttct tataccaact tattgcttct tcaatattat ttaaatcttc atagatattt
     7081 cctaatttga tttgacaaga tacattattt ggaatcctcg aataatacga aatcgctttt
     7141 tctatatctt gattctgata atataaatcc cctagatttt caagggaact tttgcatcca
     7201 acagctatag catcctcata atatttaata gcattttcat aatctttttc ttcaaaatat
     7261 atacctgcca gattattttt tgcttctgtt atatcatctt cagcagccat tttataataa
     7321 gtttttgcct tctctatctc attttctcta ttatataaat tagcaagtct atacttagaa
     7381 agtgtatgat ttttttctat tgctttctca taataataaa ttgcatctcc cagctcttct
     7441 tttctatcat atacatatgc aagcctatac tcagacttca catcatctct atcagcactc
     7501 tttttataat acttctctgc atcttctaaa ttatcattta tttccataat catacccaag
     7561 ttaaagcaac ctttcatatg acctgacttg tacaactgtt tatataaact tactgttgat
     7621 ttattatctt tcttttttcc atttaatatt gctaaattat attttgcatc atcatctaaa
     7681 agcacagaac cttttttata gtattcatat gcttcatcat ctttctccaa aaaagtatat
     7741 aataccccta aattaaaaca agctttacct aaattcttgt ctgctgctgt ttttaaccat
     7801 ttttcacacc ttacaaaatc tttcatttta aaatggcata cacctaaatt attttgtgct
     7861 tgtaaataac ctaaatttgc tatcttttca taccaatata tagcatattc atagccatct
     7921 aacttatagt acatattacc taaatcaaaa gcagcatctt cacttccact tttatatgcc
     7981 atcgaatacc acttttctgc ttctttaaat tgttttttac tttcatatat atttcctaaa
     8041 gtatattgag attttacgta attatccaat gcagcactct tatacaactc tattgcgctt
     8101 tccatcttac ccatcttttc aaggagggca cccttagtat gtacgacttt cgggtcatta
     8161 gatatatttt ttattttttt tatagaaaat tttgtatttt tcattattcg ctacctaccc
     8221 attttataaa atatattctc tcaaacatta ttatacatta taatctacaa ttaatgctat
     8281 aatattgaat ttttaccttt tgtcactata ttgtaacatt aaattttaaa ttttataatc
     8341 attagatttt attatgtaat tggtatataa tgtatatata ggggggacat tttatgaatt
     8401 tcagcaatat ttttatccaa gttgcagttt tatttataat tattttagtt ggatattttg
     8461 tcagaaaatt caacttatta gatgaccact gcacatctaa gctttcaact ttgacaatga
     8521 cagttttttt accatcaatg ataattagct ccatgcaaat taactttgat agtaaaatga
     8581 tacaaaaaat actattgtta ttatttacat ctttgattat gtatatagtt tcaattataa
     8641 ttgcattttt attgaaatac attttaaaat gtaatgataa aaaagatttg ggaatatacc
     8701 agtatatagt tgttttttca aatgttgctt ttatgggtta tcctgtaatt gaagcagttt
     8761 taggtcatga agcaatattc tatactgcaa tattcaattt gccttttaac ttattttctt
     8821 tcactttagg aatttatctt ttatctaaag gcagtacaag tagaggtttt tcaataaaat
     8881 cacttataag ccctgctaca atagctattg ttattggact ttttttattt gtaacaggac
     8941 ttaggttacc ccaatttata aacgagccac ttgagatgtt gggaagtata actacaccaa
     9001 tatccatgat tatcattgga agcttacttg ctaattcttc agctctcgat tgttttgtca
     9061 acaaaaaatt atatatagtc acttttataa gattattggt acttcctgtg attgtgtatt
     9121 ttattctaaa aggatggata aatgataaga tgattttagc tataccagta gtaatttctt
     9181 caatgcctgc tgctgcaaat actgctataa tggctaatca atatgactcc aatataacac
     9241 tagcttcaca atgtgtgttt tttacaacac tattttcagt tatatctata ccttttataa
     9301 gtattttact cttatcttga tttatttttt tatgtattat ataataacat attcaataaa
     9361 ttacaaatat aaacatttta ttgtagtata ttgatggaat tttttcctag atatttgtca
     9421 ataatatcta tatgtattat agaataggag gcttaaatat ggatatggat ttactcttta
     9481 aaatagtatt tgctatagtt gctataggtg tttctgtaaa aatcattaaa actgttgcta
     9541 gtttagcttt taaaatagcg ttgatattgc taataatttt atttgtatat agaatgtttt
     9601 tgtaaacatt taaaaataag ttttttaaca caatagagaa gtaaaataga ttatctttaa
     9661 tgtaaatttt ttattaaaga cactctattt tacttctcta ttagcttctt taaattaaat
     9721 ttttattata tcttcccata atataaacat cttcaaattt tccatctctg attacagcat
     9781 attttttagt tccctctatt tcaaaaccta gctttttata taaatttaat cccttttcat
     9841 tatccacaat tacttctaat cctactctag ttaacataat ccaattatct gctatatcta
     9901 aaagttgttc cataagcttc ttacctactc ctcttccctg ataattctct gcaacactta
     9961 ttcctatttc tgcgctatgt ctcaatcgtg gagaagaatt tacatgtaac ccagcaaaac
    10021 ctattacatc tttttctcca ttattattag tttcagctac aaacatataa tcattttctc
    10081 ccaaagaagc taacatagct tcagttttat cgactctgtc actcattaat gccaaagtat
    10141 ttctcataac actgtatgtt cttctaattt cattaaaagc ttgtgcatct tctcttctta
    10201 taggtcttat tgttatatct ataggcttac tcatattgga ttccccctca aacataatct
    10261 ttatttttaa ttaaaatttt acatctttat tttatactgt tcaaatctat gtatgcttct
    10321 acctgaggta catatatcat attatctttt atatgtgtac ttccaaattc ttttaattct
    10381 gtaccttctt tatccttagt tattttatat ctaatggatt ccataccttc aactacttca
    10441 gcatgaaaaa acataccttc atattgttta aatctaaaat taaaattgtc tttagtaagt
    10501 tttattatca tatttttatc tactccttat ataattgata tttttcttat tattagttgt
    10561 atatatactg cttattcata ttaatatatt ataacttatc atttatttag tataaagtta
    10621 taaatagata tatttatact tattttacaa tctttcttca attgatatat aatatgttta
    10681 tataaactaa tgtatgtgag gtgaaatatg agcaaaaaaa caaaatattt ttctttaatt
    10741 ctctttccta ttgctttgct tttaaatttt atagccagta aagttccaaa tattgttgaa
    10801 aaatattact cccaattcat agataaaata attgtacaaa tattgagtaa agtttctgga
    10861 attttcccat tttctctcta tgagattaca atgtacataa tagttatatc taccttctta
    10921 tttttatgtt gtactttatc tacaattttt aataagaaaa aaaaattaaa catatattta
    10981 aaaaattcca ttttaaatat attatccatt gtatcaatag tctactttct ttttgtagtg
    11041 ctttggggaa taaactataa taggattcca ctagaaacca cacttataaa taactataat
    11101 ttaaaaaaca atacctctat ccaatcaaaa caatatagtg ttaaagagct tactaaatta
    11161 tataaattcc ttgtaattaa agcaaatgaa actagaaaac tcacattaca agataaaaat
    11221 ggtattgtaa aatctaatac agattataat ggtgtcataa atagagctca actaggatac
    11281 gataatatct tggatatatt acctggtgtt agtgatagtt attctaagcc taagtatatt
    11341 atttcatcaa atctaatgtg ctatacagga ataactggta tatactttcc ttttacaggt
    11401 gaggcaaatg taaatatagc aatacctgac ttatatattc cttgtacagt tgaacatgaa
    11461 atggcacatc aaaggggatt tgctagtgaa gatgaagcta attttatagc gtatcttact
    11521 tctataaaac accccaatat tgattttaac tattctggat atattttagc tttaaattat
    11581 actgcttctg ctttaagtaa ggttgattat aatgcatatg ttgatatttc tgctggcatt
    11641 tcagattctg tccgtagaga tttaaaaaat gaaagtgaat tttggcaaaa atatgaaggt
    11701 aaaattaatg aaatttctaa tgagtttaac aattcttact taaaagctaa tggcgttact
    11761 gaaggtactc agagttatgg taaaatggtt gatttattgc taacttatta tgaattatat
    11821 ccatataact aaacactaca attcaaaatt ttattcatat acagctgata ttaaatcagc
    11881 tgttttctat ttaaataaat tatttaactt caagcaatct tatataattt gatagtactt
    11941 atactatctg tataacattt caattctaac tattttagtt aaaacatttc tattatcttt
    12001 tttaataaat gttttaacta aaatttttaa aacataaaac ttccacatta tttcactatc
    12061 tatataaaaa tctaaaaatt cacatataaa attacataac taaaaaatca taaatttata
    12121 gaaaacatat attcaaaata aaaaagcatg tatcaaaact tgacacatgc ttataatctt
    12181 atgtatatat atcattttgt cacaataaaa atttacttgt aacactgata tttttaaaca
    12241 aaatagtcct tatttaatta aattaacatt aattaaattc gtactcattt tgctatcatt
    12301 aaacattact tatagtaatt ttctaataca ctatttgaaa cttccttccc ttgaaaactt
    12361 ttcaaccaat tctcatatgc tgagtttttc catacactac catcattaaa tgttacttgt
    12421 tttacacaag atataatgta ctttacacca tgctctccag ttgattgttt ccatttatca
    12481 aacaatagaa aggcatcatt ttgattctta cctggtgcta ttgcattttg ctttgcatat
    12541 tctttctcca tttgtttcca ttctttcaac caattttcta cccatgcctt gccttgttgt
    12601 ttttcaaaca tacttattat atcctgtggt ggtgattgtt gcatttttct ataagtatgt
    12661 gaatatgatt ttggagacac aggagatatg cctgtaacaa taccatagtc tacaccagct
    12721 gggctaaatc caacacttcc tacttcgcca tctgctgcca cattttgagc atcccaatat
    12781 agctcaagtg gttttccatt tttatcatat gctaagcatg taatttcata atccataatt
    12841 gccttttcac tgctatttgt taagcttata acattgctag gccaatgata tggagtatca
    12901 tcatactgta ttttgctatt atttataaat aatggaatat tctcattata aacaagacct
    12961 ttactacttt gaatatctga taccttcttt gtgacagtac ttaccgtatt tgctaagtat
    13021 acatttgttt tttctgaatt ggtcgtacta agagaaaatg ttgttgtcgt tccagtaatc
    13081 agcaaagcaa caactaaaat aggtaagcta gatatttttt tatatctcac aattcttcac
    13141 ctctttttct attaacttat aataaaacaa tttaccaaaa cattaaaatc tatgttaaca
    13201 taatttacca actgttctag taactatatc ctatcatagt tactatcaag tttcaatact
    13261 attgatggaa attttcacta tattaaatct ttttttaatt ttataaattt acaatatgat
    13321 ttaactcata catatatagt caaattagaa tataagtttc ttacatttaa tgaagtttat
    13381 aaaataaaaa atgactagat aattaaatac tgtaaataaa aacttattac aaaagtattt
    13441 aaaaatctag tcatttaata tatatatgaa ttgtatacta taatttttca atctcttcta
    13501 gcaactcatc aactaatttg ttaccatcaa tcattctaac tatttcaccc tttttaaata
    13561 aaagaccttc acctttaccc ccagcaatac ctatatctgc ttctctggct tcaccaggtc
    13621 catttacagc acagcccata attgcaacag tgatattttt ttccatacta ccaatctttt
    13681 cttcaacctc atttacaaca ctaattaagt ctatattaca tctaccacat gtagggcatg
    13741 atataacttt aatcttatca tttaaaagtc ccaaacttct taaaatctgt ttaccaacaa
    13801 taacctcttc aacagggtcg cctgttaaag atattctaac agtatctcca attcctttaa
    13861 gaagaagtgc tcctacccct atagaagact ttattgttcc tttgtgaaca cttccagatt
    13921 ctgtaattcc aatatgaaga ggataatcaa ctttttttga tattaattca tatgcatcaa
    13981 cagttttata aatatcggat gattttagag atataactat attatgaaaa tctaagtctt
    14041 caagaatctt aatatgtccc attgcacttt caactaatgc ttcagcagtt gcagagccat
    14101 acttttttaa aagttctttc tctaaagaac ctccattaac tccaattcta atttttaaat
    14161 ttctttcctt gcatttctca actaccatct taactctctc tatacttcca atattaccag
    14221 gatttattct tattccatcc acaccttgtt ctatagcttc aagggctagc ttataatcga
    14281 aatgtatatc tgcaataatt ggtatattta cactagactt tatctttgct atatttttag
    14341 cagctttaat atcaggaaca gcaactctaa ctatatcaca accagcttcc tctaatctct
    14401 ttatttgagc tattgtagca tctgcatctc ttgtatcagt atttgtcata gattgtatac
    14461 ttataggatt ttctcctcct atttttacag ctcctacact tacttctctc gtctttcttc
    14521 tctcgtaact catattccac ctctatatat tattttatat tgaatctctt taaatattaa
    14581 aatattataa cctacatgta tattataaat taaaaagcta tctagttaaa taagaataaa
    14641 tttcatatat aaaaattaat ttattcttat ttaaaactac atagcctatt ttcttaaaat
    14701 ctctaaaaaa ctaaatcatt gaaataatct tagtatatct ttatatgtga taaaaagcat
    14761 aaataacatt aaagctccaa aaccaacaac atttatcatg ccttctttat ttgggtcgag
    14821 ttttttacca cctctaacag cttctagtaa caacataagt attctccatc catcaagtgc
    14881 tggtattggt aatagattta ctatacctaa atttaagctt atgacagctg ctaaatacac
    14941 tacatttatg atgcctgtac gagcagcatc tgataccata ccaataacac ctactggtcc
    15001 tgctacagca ttgccaatcc ctcctggtac acgtcctgta atcatttgac ctagaaatgt
    15061 tagcatctgt tttgtcatat ctacagtagt tttacatgca tttactattg accctaaaaa
    15121 gtctttttct ctttgtggtt gaattcctat ttcatacttt ccattgttat ttttaggtgt
    15181 tacatttaca actttatcac ttccatctct agttatacta agttttaatt ctccaccaga
    15241 agaagtgttt attatatttg caacttcatc ccaagtctta acagaattac cattaatttt
    15301 atttatctta tctcctactt gaagccctac agcctgtgca ggagtatctg gcattatctt
    15361 tcctaattta gtcgttggag aacctatata caaaaatact ggaactaaaa gtattgctgc
    15421 taatattata ttgaatattg gtcctgctac aatagtacta aatctttgta atatagactt
    15481 gtttccaaaa gaatttggac ttatttgttc ttcatcttca ccttccatgc tgacatatcc
    15541 accaattgga agtgctctta tagaatactc tgtatctttt tttacactat atatcttagg
    15601 ccccatacct atagaaaact catttacttt gataccactt ctttttgcaa atataaaatg
    15661 tcctaactca tgtattaata caattatact aaatagtatt aatgcggcta ttatagtcat
    15721 aaattcacct tcctattttt ttatcagatt tgcaacataa gctctactcc atctatctgt
    15781 ttctaagatt tcttctaatg ttggttcact tatgctacta tgaacttcta aagtcttctc
    15841 tatgtaatat ggtatgtcat agaaaccaat tttatcctct aaaaattcac ttactaaaac
    15901 ttcatttgct gaatttaaaa cagaagaata agttcctccc atttttaaac actcatatgc
    15961 caattttaaa catggaaaag tttctaaatc tggttcttca aaagttaaag tactaaattt
    16021 tgaaaaattc attctttcaa agctactttc cattctatct ggatatgtaa gagcatactg
    16081 tattggtagt ctcatatctg gacatcctaa ttgagcaatt attgagctat ctgtatactg
    16141 caccattgaa tgaattatgc tttgtgggtg aactactaca tctatatttt cttgttctac
    16201 tccaaataac catctagctt ctatcacttc aagtccttta ttcataagcg ttgaagaatc
    16261 aatacttatt tttcttccca tactccaatt tggatgcttc aaagcttcat ttttagttat
    16321 atttgcaagt tctccctttt tctttcctct aaatggacca ccagatgctg taagtattat
    16381 cttttctata ttttttttat tttctccatt tagacattga aatatagcac tatgttcact
    16441 atcaacagga agaatattta cattgtattt ttctgcttct ttcattacaa gttcaccagc
    16501 agttaccaac gtttctttat tcgctaatgc tatgtctatt cccttcttta tagcacaaag
    16561 agttgggact aatcctatca tacctactac agcagttaaa aggacatcca cttcatcaag
    16621 tgatgatatt atttttaatc cttccattcc tgctaaaact tctatttcta tatttttagg
    16681 caacatttct tttaatttta atgctttatt ttcctcaaaa actgttacat atttaggttt
    16741 aaactctact atctgttcta gcaataattc aatattactg ttagctgata ttgcaactat
    16801 ctcaaactta tctctatgtt ctctaaccac atccaaagtt tgtttaccta tagagcctgt
    16861 agaacctaaa attgatatct ttttcatata ataatccttt ctaataataa atataccctg
    16921 tcaaaacatt ataacactac taaaacaatt atttttacta ttcatagaaa tttcatatat
    16981 tattccacag attaaaatta atctattatt ttttgtattt tgtggaatat tataaaatat
    17041 tctagtatta ttaaatgccc ttttttattt ccacaacatc ttctatttta cagtctagtg
    17101 ctaaacatat cttctccatt gtatccatag aaacagattt atcatttcct agtctgccta
    17161 aaacagaaat acttatacct gatatattta caaattccat tttctttatt tttctgtcaa
    17221 ttaacaattt ccataatgga gcgtatgaaa tcatgttatc acctcgcaaa atatattcta
    17281 tcacatttta tctattttgg aaaattttca tctatttaga taaataatca cctgcttaag
    17341 tgtcaaataa ttttatcctt ctaatatgaa aatttaatac tatactaaat gattggtata
    17401 taacacatat gatgcattat tattaacata taaaaatttt tattggtgct aaattaaagt
    17461 aatatactat tattatattt gcagatatat aagaaagatt ttattatata tttaaagtac
    17521 ttgattataa taagagacaa gggctatgat ggatactata gaaagttaga actatactat
    17581 tttagaatga acaattaaaa taattaaaat tagtttaatc ttttattctt gataaaatca
    17641 tattaaataa atacaaaaag aatcttatta ttagaaatac tgaata
//
LOCUS       DAYAPA010000085        17487 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.085, whole genome shotgun sequence.
ACCESSION   DAYAPA010000085 DAYAPA010000000
VERSION     DAYAPA010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17487
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.085"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            85..1581
                     /locus_tag="ACGI59_002608"
     CDS             85..1581
                     /locus_tag="ACGI59_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFG9740390.1"
                     /translation="MFEFETQLKRLKHEVLTAVARLSIEENLTKENIEKIPYEIIKGE
                     KARYRCCVYKEREVVLERAQLALSLTPNSKYGDINPDSVSLDTDEQIIYIVEAACDRC
                     PINEFTVTELCRGCLAHRCKEACKFGAISYINGRAYINHDLCKACGMCKSSCQYDAIS
                     EVVRPCKSVCPTGALDFNRNTMKAMIHEDNCINCGACMSACPFGAISDKSLIAPVAKK
                     LAKKENMYAIVAPAITGQIDSNITYGQLKNAIKSLGFVDMIEASCGADAVTVHESNEL
                     IERIKSGDNYMTNSCCPGFFKYIEKMFPDEKDRISSTVSPMVATGRLVKKIDSDAKVV
                     FIGPCTAKKSEATEKSLKDSVDYVLTFEELMALFEAFNIDLSKCSNEDINDGSIFGRG
                     FGASGGLAKSVQNYIAEKGLSFDFEPIKVSGPRQIKKTMTMAKLGRLEGNFIEGMMCE
                     EGCINGAGKFLSGVKAKNTFDRKNGQSTKKSVLSNDKIKEFEDVNLER"
     gene            1715..2641
                     /locus_tag="ACGI59_002609"
     CDS             1715..2641
                     /locus_tag="ACGI59_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFG9740391.1"
                     /translation="MRKIVLKDILFLVTTLSIIVILLLVTNITTNSKIKSTSESYLKS
                     DKNSVRQNGIQRSTKSSTKNTAFKTTILNTGKSDCILVEIGNKVIMIDTGEDKNGKQI
                     VDRLKEKGINTLDYLILTHLDKDHIGGVDSVLSSVKVKNIIQANYKKDSKQYDEYIDS
                     LKKADIEPVLLKDNMNIVINGAEINIHPASKSKYESSNDYSIITNISYGAHKFLFAGD
                     AEEERLSEFINGNTLKYDFVKMPHHGRYDKLTETFLESISPQYAVITCSEKKEPEEGV
                     LKILERLNIKTFLTSNGEVVINSDGKTLSVNQ"
     gene            2759..2950
                     /locus_tag="ACGI59_002610"
     CDS             2759..2950
                     /locus_tag="ACGI59_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9740392.1"
                     /translation="MLDRFFGEGGFGGCAWWIIILFFLFLAFQDCWAEMDLCAWIPFL
                     ILLLILCECGDVFDGGCGC"
     gene            3173..3523
                     /locus_tag="ACGI59_002611"
     CDS             3173..3523
                     /locus_tag="ACGI59_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6483 family protein"
                     /protein_id="HFG9740393.1"
                     /translation="MAIVDKIFNEIKLMVARLLLGKKYSEYEEKDLVYNTEEEVILIT
                     LKRLVFQGNVNEAEEILFDKAKSVNSENMQYIAIEFYTMLMEKTDEELEAMNFSKQEV
                     YQGIEDIRKVLNLE"
     gene            3784..5289
                     /locus_tag="ACGI59_002612"
     CDS             3784..5289
                     /locus_tag="ACGI59_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HFG9740394.1"
                     /translation="MKVGFDHKKYLEEQSKYILERVNNFDKLYLEFGGKLMGDLHAKR
                     VLPGFDENAKIKVLQHMKEKVEVVICVYAGDIERNKIRGDFGITYDMEVLRLIDDLRG
                     YELDVNSVVITRFEGQPATTVFINKLERRGIKVYKHYPTKGYPSDVETIVSDEGYGAN
                     EYIETTKPIVVVTAPGPNSGKLGTCLSQLYHENKRGNVVGYSKFETFPVWNVPVKHPL
                     NIAYEAATVDLKDVNMIDSFHLETYGEMSVNYNRDLELFPVLKKIIEKITGKESIFQS
                     PTDMGVNRVGFGIIDDEVIRKASIEEIIRRYFKTACEYKKGQVDKGAYDRMKMIMEEL
                     NLKPEDRNVVLPARNYSNKLKESADKNDTCPVVALELEDGTILTGKGSDLMNGTAAVV
                     LNAIKHLANISDDMHLISPVILEPIINLKTKTLASRNVSLSCQEVLTALSICAVTNPT
                     AQFAMEKLALLKGCQAHSTTILNRDDEQTFRKLGIDVTCDPEYPSQNLYYS"
     gene            5679..6776
                     /gene="dinB"
                     /locus_tag="ACGI59_002613"
     CDS             5679..6776
                     /gene="dinB"
                     /locus_tag="ACGI59_002613"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFG9740395.1"
                     /translation="MKHYRKIIHIDMDAFYASIEQRDNKKLKGRPVIVGGNPQSRGVV
                     ATCSYEARKFGIHSAMPSAVAYNRCPYAVFVRPRFDVYKSVSEQIRDIFYRYTDLVEP
                     LSLDEAYLDVTKNKKNIDSSIEIAKQIKKDIFREVGLTSSAGVSYNKFLAKIASDLRK
                     PNGLTVITEENAQDFLDKLPVNKFFGVGKVTSNTLKNLGIKTGYDLRCLNLFELENIF
                     KKRGYELYKFARGIDDRPVEPNRVRKSVGAETTLSHNLDIDEEETRNILDELCEEVCH
                     RLKNSEKFGKTLTLKIKYEDFTKITRSLSLEHYIDEYNDIRSGVDNLLRNVEVNGKQI
                     RLLGVTISNLSDKKETYKDITLFEYMDSIQM"
     gene            7039..8175
                     /locus_tag="ACGI59_002614"
     CDS             7039..8175
                     /locus_tag="ACGI59_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9740396.1"
                     /translation="MIEIRNVTKKIGNNVILDDISLVVETGTLVVLIGSSGCGKTTTL
                     KLINKLIKPTSGEIYINGKPISQENEIELRRKIGYVIQNTGLFPHLTIKENIELIPRL
                     KKEKSVEEIEKRTLQLLEMVGLDSDEFLNKYPSELSGGQQQRIGVARAIATDAEIILM
                     DEPFSALDPITRTSLQEQLFSLQDELKKTIIFVTHDMDEALKIADKICIMKDGRIAQY
                     DTPENILRKPANDFVKDFIGEDRVWDNPEYIKARDIMIKNPIAVNSTRTVTQGIEIMR
                     TSKVDSLLIIDRAKTLKGIVTVKDMKDIDDKSILLADIMSSEPLHVNEGDNLVEILNV
                     MNRNSVGYIPVISDENKLVGLITRSSLLSVLSEQFLEMEVSVLG"
     gene            8168..9715
                     /locus_tag="ACGI59_002615"
     CDS             8168..9715
                     /locus_tag="ACGI59_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HFG9740397.1"
                     /translation="MANFFNFILLQKDKIIELLIQHMSLTVTSILIAIIVGVPLGIII
                     SRISSLRKFVLGFVNLVQAVPSMALLGLLVPILGIGSKPAIFMVVVYSLLPIVKNTYI
                     GITSIDPVVLESAKGIGLTRNQTLFKIQFPLALPIIMGGVRISAVTAVGLMTLAAFIG
                     AGGLGYLVFSGVQTVNNNMILAGAIPSCIIALLVDYLFSKIEVAVTPKGLNPKAPKKN
                     YIALKVISVVVVVSMLFVVFSSSFSSKKDTITIGSKDYTEQLILGNVYAELVEKNTNL
                     KVKKNLNLGGSSVAFNAIKSGELDMYVDYTGTLLVNVMKHAPIKDADEAYNVVKDTME
                     KENQLTLLDPLGFNNTYTLAMMPETAEKYGINTISDLTKYGKEFTFSPTLEFENREDG
                     LVGLSRDYGLKFKDVKAMNGSLRYTALDNNESQVIDAFLTDGLIKKFNLKILEDDKNF
                     FVPYYAAPLVREDTLKKYPELEKVMNMLSGKINEETMRELNYQVDELGKSPEEVAHSF
                     LVKEGLV"
     gene            10050..10916
                     /locus_tag="ACGI59_002616"
     CDS             10050..10916
                     /locus_tag="ACGI59_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFG9740398.1"
                     /translation="MDNYKKVKQVLWIILFANFAVALLKIIIGNQIKSYSMTADGFHS
                     LSDGASNIVGLIGIFFASKPKDKNHPYGHKKFEIITSLFISGMLFVIAIKIILSAVLR
                     IANPVVPAITIESLIALIITLFINIFVCMYEYRIGTKLNSYVLISDSLHTRSDIFVSL
                     GVLVTLVGVKLGFPVIIESIVPIIISAFIIYSAYGIFRPSIGILVDRVAVDEDYIKEI
                     VFEFNEVRDVHNIRSRGSKSSIYIDMHVMVDPFISVEQSHDLTHKIEKQIQEEINENA
                     QVIVHIEPFYSF"
     gene            11057..12199
                     /locus_tag="ACGI59_002617"
     CDS             11057..12199
                     /locus_tag="ACGI59_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFG9740399.1"
                     /translation="MRYVEYGKTGKMVSVVGYGGLRFDLEKSNQENADLVKYAYEKGI
                     NYFDTAPGYCDDRSEDIFGLAFRQMIKEGKTDFYVSTKEKPKACDTSEKAIEAVKKSI
                     ERLGVSKINFYHVWNIRKMEHYELAMRPGGQYEGLLKCKEEGLIDHIVFSSHQPGDEV
                     IDILSENKFEGVTMGINILNFPYRLKGAKYAVDNGYGVVAMNPLSGGTIPKYNKELSF
                     LSKDGESTIESALRFNIGISQISISLIGFNKKQDIDDACKIADENRIYSDEDIHDIET
                     RLNKNMNEICTGCGYCKVCPKGINTPAYMLFYNEKQMFKKSDEEMTKLVYGLGHWNYT
                     MNSKAKAKECISCGKCEVECTQHLPIIDRLKEIKKWEEDGANSVKV"
     gene            12601..13821
                     /locus_tag="ACGI59_002618"
     CDS             12601..13821
                     /locus_tag="ACGI59_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6080 domain-containing protein"
                     /protein_id="HFG9740400.1"
                     /translation="MSKILKYKNEMFLFILVVITYLIITKIFFSKTTIFYDLNNTYDV
                     LLDTDTGVLFNLNVFAISQDNSKHILFSAIISIFAYPIYLFCTSIANPGTTDFNSAYG
                     FGLICLQIITSAMSITLVFNHIKKIKMQRLTLILLTMIMIFSFPQLFMTLNVERFIYS
                     QFSLIFFIVIANKMKGKNSYLVELAAIPLFGITISNIYLYFFNMIFEFKLKIGKMLKH
                     LITFILMAYICVVSTKSYESFMNLGNVIQYDTKFISGEPILEKIAMIIERLLYSVFYF
                     PGASIKKGLFLQNGEVATIPVILTLLALCFCVLSVIENSEKRVPKLCMGIIIFNLTLH
                     GIVGYNLVNSSIMAINFSFAVIILLAYFTKALRKNEKNMYNIFLSLLLVTIVISNVNG
                     FIEILNIGIKSYPV"
     gene            13850..14737
                     /locus_tag="ACGI59_002619"
     CDS             13850..14737
                     /locus_tag="ACGI59_002619"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HFG9740401.1"
                     /translation="MKQTNTSIRLLTKISDYIKLMRIKQYIKNFFVFSAIIFSNNILN
                     INLFLNTFIAFVCFCLMSSSVYALNDAIDMDKDKKHPKKCNRPVASGRISKKSANILF
                     VVLALISVCLSTVVSFNLSIILSLYLINNILYSLKLKNIILLDVFSISLGFILRVYAG
                     CVAISVSLSNWIILCTFFLSLYLALGKRKKEIETLRDDAVEHRKILEDYDIENLNQMM
                     IVILSSTIVCYALYSTSNPEKPHMIFTTIFVVYGVLRYNYIINTTNENNPTDIVLKDN
                     ALKINVILWIITCLIILIF"
     gene            14771..15400
                     /locus_tag="ACGI59_002620"
     CDS             14771..15400
                     /locus_tag="ACGI59_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HFG9740402.1"
                     /translation="MKKSVLLLDVDYTVINTDSMIDFFIYSLKNKTFKTIIKLPYIIF
                     ILFMYLIRMIPLKKAKEAIFYPIVDFSEEDLKKFFDDCIMKKINESMKKVIYKNKEED
                     NVIIMITASPYAYMKYFKYYGLADEVIGTEFFYENSRYKNQFIGENCKGIEKVKRIKA
                     VLGKLGIEIDYENSYAYSDSKSDLPMLSLTKNAFLVSKKDGEVIEQINS"
     gene            15432..15869
                     /locus_tag="ACGI59_002621"
     CDS             15432..15869
                     /locus_tag="ACGI59_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="COG2426 family protein"
                     /protein_id="HFG9740403.1"
                     /translation="MGYLHILILSMVPVTELRGAIPIGIAMGLNPIWVYVFSVIGSTL
                     VSIPLILTFRHVLQFLRGKKLFKGIADVIDRKINSRMKKLKSVSIIGIILFVGIPLPT
                     TGTWTASAIASILKMRIKDAFMGVFLGNLLSGVIVSALSLHII"
     gene            16119..17486
                     /locus_tag="ACGI59_002622"
     CDS             16119..17486
                     /locus_tag="ACGI59_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFG9740404.1"
                     /translation="MENSNTNLGSESVGKLLFKLATPAIIAQIVNVLYNIVDRIFIGR
                     MENGEVAMAGVGVAFPIIIIITACSYLIGMGGGPLAAIKMGEQNNDEAEKIMSNSFSV
                     LVILAILLTIGFKIGKEPLLWMFGASESTIGYSMDYLNIYLIGTVFVQISMGMNTFIN
                     TQGFATTGMMTVAIGALINIILDPIFIFGFNMGVKGAALATIIAQGVSAIWVLMFLFG
                     KKSILKIKKKYMIPKASIILPVLGLGISPFIMQSTESLVLIALNSKLQMYGGDLAVGS
                     MAIMSSIMQILMLPNMGVTQGAQPIISYNYGSGQLDRVKKTFKLCLLSCFTYSTILWL
                     LLMIFPAFFVSIFNKNPQLLSMTSWSIKIYFAGAFMFGIQIACQQTFLALGKATISLV
                     LALLRKIVLLIPLIFILPTFFNEKLFAVILAEPVADITAATITAISFFIFYKCFLSKP
                     KAIKE"
BASE COUNT         6690 a         1818 c         3065 g         5914 t
ORIGIN      
        1 taatttatag aaataaataa aaaatacaat atacataata gtagaaatat agcaaaaagg
       61 tgtgatttat agggaggatt acttatgttt gaatttgaaa cgcaactaaa aaggttaaaa
      121 catgaagttt taacagcagt agcaagacta tctattgaag aaaatttaac aaaagaaaat
      181 atagaaaaaa ttccatatga aataattaaa ggagaaaagg cgagatatag atgttgtgtt
      241 tataaagaga gagaagtagt tcttgaaagg gcgcaacttg cattaagttt aactccaaat
      301 agtaaatatg gagatataaa tccagattca gttagtttgg acacagatga gcaaattata
      361 tatatagttg aagcagcatg tgatagatgt ccaataaatg aatttacagt tacagagctt
      421 tgtagagggt gtttggctca tagatgtaag gaagcatgta aatttggggc aatttcttat
      481 ataaatggga gagcatatat aaatcatgat ttatgtaagg cttgtggaat gtgcaaaagt
      541 tcatgtcaat atgatgctat atcagaagtt gtaagacctt gtaaaagcgt ttgtccaaca
      601 ggagctttgg attttaatag aaacactatg aaagcaatga tacatgaaga taattgtata
      661 aattgtggag catgtatgtc agcatgtcct tttggagcaa tatctgataa gagtttgata
      721 gcacctgttg caaaaaaact tgcaaagaaa gaaaatatgt atgcaatagt agctccagct
      781 ataacaggtc aaatagatag taatattaca tatggtcagt taaaaaatgc tataaaatca
      841 ctgggatttg tagatatgat agaagcttct tgtggagcag atgcagttac agttcatgaa
      901 agcaatgaac ttattgaaag aataaaatca ggagataatt acatgacaaa ttcatgttgt
      961 ccgggatttt ttaagtatat agagaagatg ttcccagatg aaaaagatag aatatcatct
     1021 acagtgtctc ctatggtagc aacaggaaga ttagtaaaaa aaatagattc tgatgctaaa
     1081 gttgtattta taggtccatg tacagctaaa aagagtgagg caacagagaa aagtttaaaa
     1141 gattcagtag attatgtact tacttttgaa gaactaatgg cattatttga agcatttaat
     1201 atagatttat caaaatgttc aaatgaagat ataaatgatg gttcgatttt tggaagggga
     1261 tttggagcat ctggtggact tgccaaatca gtacagaact atattgctga aaaggggctt
     1321 agttttgatt ttgaacctat aaaagtatct ggtccaagac aaattaaaaa gacaatgaca
     1381 atggctaaat tgggaagatt agaaggtaat tttatagaag gaatgatgtg tgaagaaggt
     1441 tgtattaatg gagccggtaa gtttttatca ggtgtaaaag ccaaaaatac ttttgataga
     1501 aaaaatggac aatctactaa aaagagtgtt ttatctaatg ataagattaa agaatttgaa
     1561 gatgtaaatt tagaaagata attgttttgg tacagtcatt tttttattat gccacaaata
     1621 tgcctaaaat ttgccctttt tatatgtgta ttttacagag ttaaggttgc tatactataa
     1681 atatgtaata aataaaattt ataggagctt ttggatgagg aaaatagtat taaaagatat
     1741 cctgttttta gttactacat tatctataat agtaatatta ttgcttgtaa caaatataac
     1801 tactaatagt aagataaaat caacaagtga atcttattta aaaagtgaca aaaatagtgt
     1861 gaggcaaaat ggtatccaga ggagcacaaa gagtagcaca aaaaatactg catttaagac
     1921 tactatacta aacactggaa agtctgattg catattggtt gagataggta acaaagtcat
     1981 aatgattgat acaggagaag ataaaaatgg aaaacaaata gtggataggt taaaagaaaa
     2041 aggaataaat acacttgact atttgatttt aactcattta gataaggacc atataggtgg
     2101 agtagatagt gtgttatcta gtgttaaagt aaagaatatt atacaagcaa attataagaa
     2161 agatagtaag caatatgatg aatatattga ctcacttaaa aaagctgata tagaaccagt
     2221 gctactaaaa gataatatga atattgtaat caatggtgca gaaataaaca tacatccggc
     2281 ttcaaaaagc aagtacgaga gttcaaatga ttattcaata atcactaata taagctatgg
     2341 agcacataag tttttatttg caggagatgc agaggaagaa agattgtcag agtttataaa
     2401 tggaaatact ttaaaatatg attttgttaa aatgcctcat catggaagat atgacaaatt
     2461 aactgaaaca tttttagagt ctatttcacc tcaatatgct gttattacat gttcagagaa
     2521 aaaagaacca gaggaaggtg ttttaaaaat attggaaaga ttgaatataa aaacattttt
     2581 gacttctaat ggagaggtag ttatcaatag tgatggtaag acattgagtg tgaatcaata
     2641 atttttttaa aaatatctaa aaaaatttaa aaaaatcatt agcaaatatt accccttaaa
     2701 catagtatat tttaggagct gaaactccac atacaaaatg actaggaggt aacatattat
     2761 gttggataga ttttttggtg aaggcggatt tggtggatgt gcttggtgga taataatatt
     2821 attcttctta ttcttagcat tccaagattg ttgggctgaa atggacttat gtgcatggat
     2881 acctttctta atattattat taatactttg tgaatgtggt gacgtttttg atggaggatg
     2941 tggatgctaa aaaaatcaat ttagatgatt taataaagct tatggtgggt ataaataaaa
     3001 taccaaacca atcacaatct agtgtttgaa ggtagtcgga aatcccccgc taccttcttt
     3061 tttttattgt taaaatttat ttttgaatta tttcatttac atataataat ttttacgata
     3121 aagtctgata taattaaaaa gaaaataaaa tttatttata ggagatgtaa aaatggctat
     3181 agtggataag attttcaatg agataaaact tatggttgca agacttttat tgggaaaaaa
     3241 atacagtgaa tatgaagaaa aagatttagt gtataataca gaagaagaag ttatacttat
     3301 aactttaaaa agattagttt ttcagggaaa tgtaaacgaa gcagaagaaa ttttatttga
     3361 taaagctaag agtgtaaata gtgaaaatat gcagtatata gcaatagaat tttatactat
     3421 gcttatggaa aagactgatg aagaattaga agctatgaat ttttctaaac aagaagttta
     3481 tcaaggtata gaagatatta gaaaagtatt gaatttagaa taaaagatat atgaaaatat
     3541 tgaaataaat taatgctaag gtgagaatat tatacttata tataaaaata tagtattctt
     3601 attttattag ttatataggt atatcttatt taccataaaa ctatcttgaa aaaaattaat
     3661 cttaattgta taatattact ttgagtgaaa aagtattaac atttttttat aagatgtttt
     3721 actaaaaaaa tatatataaa tatactgtta tcaacaagaa atttaattta gtgaggtgtt
     3781 aagatgaaag taggatttga ccacaaaaaa tatttagaag agcagtctaa atatatctta
     3841 gaaagagtaa ataattttga caaactttat ttagagtttg gaggaaaatt gatgggagac
     3901 cttcatgcaa aaagagtact tccaggattt gatgaaaatg caaagataaa agtattacaa
     3961 catatgaaag aaaaagttga agttgtaatt tgtgtctatg caggagatat agaaagaaac
     4021 aaaataagag gagactttgg tatcacttat gatatggaag ttttaagatt aatagatgat
     4081 ttaagaggtt atgaactaga tgtaaatagt gtagtaatta ctagatttga aggacaacct
     4141 gctacaactg tatttataaa taaattagaa cgtagaggaa ttaaagttta taagcattat
     4201 ccaacaaagg gatatccgtc agatgttgag actatagtta gtgatgaggg atatggagca
     4261 aatgaatata tagaaactac aaagccaata gtagtagtca cagcaccagg acctaatagt
     4321 ggaaaacttg gtacttgtct aagtcagttg tatcatgaaa ataaaagagg taatgtagtt
     4381 ggatattcta agtttgagac attcccagtt tggaatgtac ctgtaaaaca tcctttgaat
     4441 atagcttatg aagcagctac agttgattta aaagatgtga atatgataga ttcattccac
     4501 cttgaaacgt atggtgaaat gtcagtaaac tacaatagag atttggaatt atttccagtg
     4561 cttaaaaaaa taatagaaaa gataactgga aaagaatcaa ttttccaatc tccaactgat
     4621 atgggtgtta atagagttgg ctttggaata atagatgatg aagtaataag aaaagcttct
     4681 atagaagaaa taataagaag atactttaaa actgcctgtg agtataaaaa gggacaagtt
     4741 gataaaggtg cttatgatag aatgaaaatg attatggaag agcttaactt aaagccagaa
     4801 gatagaaatg ttgtattacc tgctagaaac tacagtaata aattgaaaga aagtgccgac
     4861 aagaatgata cttgtcctgt agttgcatta gaattagaag atggtacaat attaactggt
     4921 aagggttctg atttaatgaa tggaactgca gcagtagtat taaatgccat taaacattta
     4981 gccaatatat ctgatgatat gcacttaata tcacctgtaa tattagaacc tatcataaac
     5041 ttaaaaacaa agactttagc tagtagaaat gtttcactta gttgccaaga ggtgttaact
     5101 gcacttagta tttgtgctgt aactaatcca actgcacaat ttgctatgga aaagttagca
     5161 ttattaaaag ggtgtcaagc tcattctact actatattaa atagagatga tgaacaaaca
     5221 tttagaaaac ttggtataga tgtaacttgt gacccagaat atccttcaca aaatctttac
     5281 tatagttaaa attatataaa aaaatatggg ctgttttaaa gtaatagttt gagcagtcca
     5341 ttacaattat aataaaatca gtaggctgtc ttgtataaga cagtcttttg tttttaaaac
     5401 atcccataaa ttaaatatat atactgttat ccataaggaa ttttaaattt agttccttgg
     5461 aatacctatt gtaaagcttt tatatagttt ttgcaagtgt ttttaaggat tttttataaa
     5521 ttatattgaa gttaatggat tatagatggt tttatttatg gaaaatatga atatataaag
     5581 aaaataatga aaaaatacca aaaagaaaca taagtttggt ataattaaaa agtaatataa
     5641 ataaaattaa tctatttata ctttaaaagg tgataataat gaaacattat agaaaaataa
     5701 ttcatataga tatggacgct ttttatgcat ctatagaaca aagagataat aaaaaattaa
     5761 aaggaagacc tgttattgtc ggtggaaatc cacaaagccg aggtgtggtt gcaacctgtt
     5821 cttatgaagc tagaaaattt ggtatccatt cagctatgcc atcagctgta gcttacaata
     5881 ggtgtccata tgcagttttt gtaagaccta gatttgatgt ttataagagt gtgtcagagc
     5941 aaataagaga tatattttat agatacacag atttagttga accattatct ttagatgaag
     6001 cttacttaga tgtaacaaaa aataaaaaaa atattgattc atcaattgag atagcaaagc
     6061 aaataaaaaa agatatattt agagaagttg gtcttacttc ttcagctggt gtgtcataca
     6121 ataagtttct tgctaagatt gcatcagact taagaaagcc aaatgggtta actgtaataa
     6181 ccgaagaaaa tgcccaggac tttttagata aattacctgt gaataaattt tttggtgttg
     6241 gaaaagttac ttcaaatact cttaaaaatt taggtataaa aactggatat gatttaaggt
     6301 gtttaaattt atttgaactt gaaaatatat tcaaaaaaag aggttatgaa ttatataaat
     6361 ttgctagagg aatagacgat agaccagtgg aaccaaatag ggttagaaaa tctgttgggg
     6421 ctgagactac attaagtcat aatttggata ttgatgaaga agaaacacgc aacattcttg
     6481 atgaactttg tgaagaagta tgtcatagat taaaaaactc tgagaagttt ggaaaaacat
     6541 taacattaaa aattaaatat gaggacttta caaagataac tagaagcctt agtttagaac
     6601 actatattga cgaatataat gatataagaa gtggtgtaga taatttgttg agaaatgttg
     6661 aggttaatgg aaaacaaatt aggctattgg gagttacaat atcaaaccta agtgataaaa
     6721 aagaaactta taaagatatt acattatttg aatacatgga tagtatccaa atgtgagttt
     6781 tgtttaaatg aaataacact agctttaata ctatataatt gaggctagtg ttattttatg
     6841 aatattttta tattttttgt tttttgacat tgtatattat atactatata taaatacttt
     6901 atatttgtag caatattcag acaaatagtg acctaaaaat cgtactttgt ttacataaat
     6961 aaaatagtat gatataatta taaaaacaac ttataatatt ggagaaggtt gcttgttcga
     7021 aaaaggggat gataaattat gatagagata agaaatgtaa caaaaaaaat aggaaataat
     7081 gttatattag atgatatttc tctagttgta gaaacaggta cattggtagt tttgattggt
     7141 tcaagtggtt gtggaaagac tactactttg aagcttataa acaaattaat aaaacctaca
     7201 tctggtgaga tatatattaa tggcaaacca atttctcagg aaaatgaaat agagcttaga
     7261 agaaaaatag gatatgttat acagaacaca ggcctattcc ctcatcttac aataaaagaa
     7321 aatatagagc taattccacg cctaaaaaag gaaaaatccg ttgaagaaat tgaaaaaaga
     7381 acgttgcaac tacttgagat ggtaggactt gatagtgatg aatttttgaa taagtatcct
     7441 tcagaactta gtggtggaca gcaacaaaga atcggtgttg caagagccat tgctactgat
     7501 gctgaaataa tattgatgga tgagcctttt agtgctttag acccaataac gagaacatct
     7561 ttacaagaac aattattttc tcttcaagat gaattgaaaa aaactataat atttgtaacg
     7621 catgatatgg atgaggcttt aaagatagct gataaaattt gtattatgaa ggatggaaga
     7681 atagcacaat atgatactcc cgaaaatata cttagaaagc ctgcaaatga ctttgtaaaa
     7741 gattttatcg gagaagatag agtttgggat aatccagaat atataaaagc aagagatatt
     7801 atgataaaaa atcctattgc tgttaattct actagaactg taactcaagg aatagaaatt
     7861 atgagaacga gtaaggttga tagcttacta attatagata gagcaaaaac attaaaaggt
     7921 attgttactg taaaagatat gaaagatatt gatgataagt caatattact tgctgatatt
     7981 atgtcaagtg agcctttgca tgtaaatgaa ggggataatt tagtagaaat actaaatgta
     8041 atgaatcgta attcggttgg atatattccg gttatttctg atgaaaataa actggtaggg
     8101 cttattacta gaagtagttt actttctgtt ttaagtgaac aattcttaga aatggaggtg
     8161 agtgttcttg gctaattttt ttaactttat actattacaa aaagataaga ttatagagct
     8221 tttaatacaa catatgagtc taactgttac atctatttta atagctataa tagttggtgt
     8281 tcctcttgga ataattattt caagaatctc atctcttaga aagtttgtac ttggatttgt
     8341 aaacttagtc caagcagttc cgtctatggc acttttgggt cttcttgtac ctatacttgg
     8401 tataggaagt aaaccagcca tatttatggt agtggtttat tcattactac caattgtaaa
     8461 aaatacgtat ataggtataa caagtattga cccagtagta ttagaatcag ctaaaggtat
     8521 tggtcttaca agaaatcaga cattatttaa aatacaattc cctttagcac ttcctataat
     8581 aatgggtgga gttagaatat ctgcagttac agctgttggt ttgatgactc ttgcagcatt
     8641 tataggtgct ggtggtttag gatatttagt attttcaggg gttcaaactg taaataataa
     8701 tatgatttta gcaggggcaa tcccatcatg tataattgca ctgttagttg attatttatt
     8761 tagtaaaatt gaagttgcag ttactccaaa aggattaaat ccaaaagcac ctaagaagaa
     8821 ttatatagca cttaaagtaa tatctgtagt ggttgttgtt tcaatgttgt tcgttgtttt
     8881 tagttctagt ttttcaagta aaaaagatac aataactatt ggttctaaag attatacaga
     8941 acaacttata cttggtaatg tttatgctga attagtagaa aaaaatacga atcttaaagt
     9001 taagaagaat ttaaatctag gtggttcatc tgttgcgttt aatgcaatta aatctggtga
     9061 gttagacatg tatgttgact atacaggtac acttttggta aatgtaatga aacatgctcc
     9121 gattaaagat gctgatgaag catataatgt tgtaaaagat actatggaaa aagaaaatca
     9181 gttaacttta cttgaccctc ttggatttaa taatacttac acattagcta tgatgcctga
     9241 aactgctgaa aagtatggaa taaatactat ttctgatttg actaaatatg gtaaagaatt
     9301 tacattctca ccaactttgg agtttgaaaa tagagaagat ggtcttgtag ggctttctag
     9361 agattatggt ttaaaattca aagatgtaaa agcaatgaat ggaagtttaa gatatacagc
     9421 tttagataat aatgaatctc aggttataga tgcattttta actgatggtc ttattaaaaa
     9481 gtttaatctt aagatattag aggacgataa aaatttcttt gtaccatatt atgcagcacc
     9541 attagtaaga gaagatactt tgaagaaata tcctgagtta gaaaaagtta tgaatatgct
     9601 ttcaggaaaa ataaatgaag aaactatgag agaacttaac tatcaagtgg atgaacttgg
     9661 taaatcacca gaagaagttg ctcattcatt cttagttaaa gaaggcttag tataaaaaat
     9721 taatagttta tatcaaaaaa tttaagagag tgtctcaaaa tgaacttttt agttcatgag
     9781 gcactctttt ttatatgaaa tcaaatatat tttatcattt caacaataaa aaagaagatt
     9841 atctttattt tgagacagtc tcttattttt atgtatacca aaaccatacc tacgattttt
     9901 atttataatt aagtttcaaa tagaatgaac aagtacaacc aattttgaat ataaatatag
     9961 taaatgtgct tatatacgat tttattgtct ggttgattga tattgataga tagctttctt
    10021 tattgaatcg aagggagggg gaatttgtta tggataacta taagaaagtg aagcaagtat
    10081 tgtggataat tttatttgct aattttgcag tagcattatt aaaaatcatt attggtaatc
    10141 aaataaaaag ctatagtatg acagcagacg gatttcattc tttgtcagat ggagcatcta
    10201 atatagtggg gcttataggt attttctttg catctaaacc aaaagataaa aatcatcctt
    10261 atggacataa aaagtttgaa attataacca gtttatttat aagtggtatg ttgtttgtta
    10321 tagcaattaa gataattctt agtgcagtgt taagaatagc taatccagtt gtacctgcta
    10381 taactataga aagtttaatt gctttaatta ttacactttt tataaatatt tttgtatgca
    10441 tgtatgaata tagaattggg actaaactaa atagttatgt tttgatttcg gattcattgc
    10501 atacaagaag tgatatattt gtgtctctag gtgtattggt tactttagtt ggtgttaagt
    10561 taggatttcc agtaataata gaatctatag ttcctattat tatttctgca ttcataattt
    10621 attcggctta tggaatattt aggccatcta ttggaatttt ggtcgataga gtggctgtag
    10681 atgaggatta tataaaagaa attgtgtttg aatttaatga ggtaagggat gttcacaata
    10741 tcagaagtag aggtagcaaa agttctattt atattgacat gcatgtaatg gtagacccat
    10801 ttataagcgt agagcaatct catgatttaa cacataaaat tgagaaacaa atacaagaag
    10861 aaattaatga aaatgctcaa gtaatagtcc atattgaacc tttttatagt ttttaactta
    10921 gctataaaat acaagttata aaaaaatata atttataaaa aaaatattat accataaata
    10981 atttataaaa attatttatg gtataatatt ttctataaag aaattaagtc ttaatatatc
    11041 taaggaggaa tttacaatga gatacgttga atatggaaag acagggaaaa tggtttcagt
    11101 agttggctat ggaggattaa ggtttgattt agaaaaaagt aatcaagaaa atgctgattt
    11161 agtcaaatat gcgtatgaaa aaggtattaa ttactttgat acagcaccag ggtattgtga
    11221 tgacagaagt gaagatatat ttggactagc ctttagacaa atgataaagg aaggtaagac
    11281 cgatttttat gtatcaacta aagaaaagcc aaaagcatgt gatacatcag aaaaagctat
    11341 agaggctgta aaaaaatcaa tagaacgatt aggcgtatct aaaataaatt tttatcatgt
    11401 ttggaatatt agaaagatgg aacattacga actagcaatg agacctggag gtcaatatga
    11461 aggattattg aaatgtaaag aagaaggatt gatagaccat atagtatttt catctcatca
    11521 accaggagat gaggttatag atattctaag tgaaaataag tttgaaggtg taactatggg
    11581 aataaatata ttaaactttc catataggct aaaaggtgcc aaatatgctg ttgataatgg
    11641 atatggtgtt gtggcaatga atccattaag tggtggaact attccaaagt ataataaaga
    11701 actttctttt ctatcaaaag atggcgaaag tactatagaa tcagctttaa gattcaacat
    11761 aggaatttca caaataagta tatctttgat agggtttaat aaaaaacaag atatagatga
    11821 tgcatgtaaa attgcagacg aaaatagaat ttattcagat gaggatatac atgatattga
    11881 gactagatta aataagaata tgaacgaaat atgtacaggt tgtggttatt gtaaagtatg
    11941 tccaaaaggt attaataccc cagcctatat gcttttttat aatgaaaagc agatgtttaa
    12001 aaagagtgat gaagaaatga caaagttagt ttatggctta ggacattgga attacacaat
    12061 gaatagtaag gcaaaagcta aagaatgtat atcttgtgga aaatgtgaag ttgaatgtac
    12121 tcagcattta cctataatag atagattaaa agaaattaaa aaatgggaag aagatggagc
    12181 taatagtgtt aaagtctaat taattttata ctatatttga aatttgtata gtatttagta
    12241 atgttttaaa atatagtttt aatatttaag gagacagatt ataattttgt ctccttaaat
    12301 tatgctttaa tttgttatag tttaattcca taagtaacat tgtatatttt tatataaagt
    12361 ttttcaaata ttttacatta atattgctta caaattctca ttttatacaa cactaatcta
    12421 tgttatataa acaatacttg actgattata acatcaataa agtaaatatt gtggttgtaa
    12481 ataaagagtt attcaattac aataaaacaa gtaagaacat tatgaagatg gctattaaat
    12541 ataaggaata aattgtaggt gtcagcaaat cctatataat gaatggatgg tgatatattg
    12601 atgagtaaaa tattaaaata taaaaacgag atgtttttgt ttatattagt tgtaataact
    12661 tacttaataa taactaagat atttttttct aagactacta tattttatga tttaaacaat
    12721 acttatgatg tattgttaga tacagataca ggagttttat tcaatttaaa tgtatttgct
    12781 ataagtcaag acaatagtaa gcatatattg ttttcagcaa ttatatcaat ttttgcatat
    12841 ccaatatacc tattttgtac ttcaatagca aatcctggga caacagattt taatagtgcc
    12901 tatgggtttg gactaatttg tctacaaata ataactagtg ctatgtcaat aactttagtc
    12961 tttaatcata ttaaaaagat aaaaatgcag agattaactt taattttact tactatgata
    13021 atgatatttt cttttccaca gttatttatg actttaaatg tagagagatt tatatatagc
    13081 caattttcat taatattttt catagttatt gcaaataaga tgaaaggaaa aaattcttat
    13141 ttggtagaat tagcagctat tcctctattt ggaataacta ttagtaatat atatttgtat
    13201 ttttttaata tgatatttga gtttaaatta aaaataggaa aaatgttaaa acatttaatt
    13261 acatttatcc taatggcata tatttgtgta gtttcaacga agtcttatga gagttttatg
    13321 aaccttggaa atgtaataca gtatgatacg aaatttataa gtggagaacc aattttggaa
    13381 aagattgcaa tgattataga gagattattg tattcggttt tttattttcc tggagcaagt
    13441 attaaaaaag ggttattttt gcaaaatgga gaagttgcta caataccagt aattcttaca
    13501 ttattagctt tatgtttttg tgttttatca gttattgaaa attcagaaaa aagagttcct
    13561 aagctatgta tgggaataat tatattcaat ctaactttac atggtatagt aggatataat
    13621 ctagtcaatt caagtattat ggctataaat tttagttttg cagttataat tttattggct
    13681 tactttacga aagcccttag aaaaaatgaa aagaatatgt ataatatatt tctatcgctg
    13741 ttattggtta ctatagtaat cagtaatgtc aatggattta tagaaatttt aaacataggg
    13801 ataaaatcat atcctgttta aaaaataata ttgaaagggt agtaagttta tgaaacagac
    13861 caatacaagt ataaggcttc ttactaaaat ttcagattat ataaagttaa tgagaataaa
    13921 gcaatatata aagaatttct ttgtattttc agctataatt ttttcaaaca acatactaaa
    13981 tataaattta tttttaaata cgtttattgc gtttgtgtgt ttttgcctaa tgtcatcatc
    14041 agtatatgca ctgaatgatg ctatagacat ggataaagat aaaaaacatc ctaaaaaatg
    14101 taatagacca gtggcaagtg gaagaataag caaaaaaagt gctaatatac tatttgtagt
    14161 attagcgtta atttcagtct gtttatcaac agttgttagt tttaatcttt ctataattct
    14221 cagtctctat ttgataaaca atatattata ttctcttaaa ctaaagaata taatactatt
    14281 agatgtgttt tcaatatctc ttggttttat acttagggtt tatgcaggat gtgtagccat
    14341 aagtgttagt ttatcaaatt ggataatttt atgtacattt ttcttatctc tgtacttagc
    14401 tcttggaaaa cgtaaaaaag aaatagaaac tctaagagat gatgcagttg aacatagaaa
    14461 aatattggaa gattatgaca tagaaaatct aaatcaaatg atgatagtta tattaagttc
    14521 tacaattgta tgttatgcat tgtattctac aagtaatcca gaaaagccac atatgatatt
    14581 tacaacaata tttgtagttt atggtgtact tagatataat tatataatta acactacaaa
    14641 tgaaaataat cctactgata ttgtcctaaa agataatgct ttaaaaataa acgtaatctt
    14701 atggataatt acatgtttaa taatattgat tttttagtga tatttttagt gataggatgg
    14761 ggaaatactt atgaaaaaaa gtgtattatt acttgatgta gattatactg ttataaatac
    14821 tgatagcatg atagattttt ttatttactc tttaaaaaat aaaaccttta aaactattat
    14881 aaagttgcca tacattattt ttattctatt tatgtattta attagaatga tacccttgaa
    14941 gaaggcaaaa gaggcaatct tttatccaat agtagatttt agtgaagaag atttaaaaaa
    15001 gttttttgat gattgcataa tgaaaaaaat taacgaatct atgaaaaagg ttatttacaa
    15061 aaataaagaa gaagataatg ttataattat gataactgca tctccatatg catatatgaa
    15121 atattttaag tattatggtc ttgcagacga ggttataggc acggagtttt tttatgaaaa
    15181 ttcaagatat aaaaatcaat tcattggaga aaactgtaag ggaattgaaa aagtaaaaag
    15241 aataaaggct gtattaggta aacttgggat agagattgat tatgaaaatt catatgcata
    15301 ttcagactct aagagtgatt taccaatgct ttcattgact aaaaatgcat ttttagttag
    15361 taaaaaggat ggtgaggtta tagagcaaat aaattcataa cttaatgtca cttagaatag
    15421 gagggaatag tttgggatat ttacacatat taattttatc tatggttcct gtaacagaat
    15481 tgaggggagc cattcctata ggtattgcaa tgggcttaaa tcctatatgg gtttatgtat
    15541 ttagtgtaat aggttctaca ttggtatcga ttcccttgat attgacattc agacatgtat
    15601 tacaattttt aagagggaag aaactattta aagggattgc tgatgtaatt gatagaaaaa
    15661 taaactcaag aatgaagaaa ttaaaaagtg tttcgataat tggtataata ctatttgtag
    15721 gtataccact gccaactaca ggaacatgga ctgcatctgc tatagcttcc atattaaaaa
    15781 tgagaataaa agatgctttt atgggagtat ttcttggcaa tttgttatca ggggttatag
    15841 tttcagctct ttctttacat ataatataaa actatctaag taatatactt ggatagtttt
    15901 tttatgagta tatgtgaaaa atttttaata ataaataaaa aatgagataa agtatttaac
    15961 ttcagttagt tctaaaatgt ttactatggc aaaagaaata tagtatagta ttacttgtag
    16021 ggaaagtact ctaaatttgt ctgctaataa aatgaaattt ttatatagaa taaaatttta
    16081 gtgatacatt ttaattattg gattgaggag gttttaaaat ggaaaattca aacactaact
    16141 taggtagtga aagtgtgggt aaattattat ttaaacttgc tacacctgct attatcgcac
    16201 aaattgtaaa tgttttatac aacatagtcg ataggatttt tattggtaga atggaaaatg
    16261 gagaagttgc tatggctggt gttggagtgg catttccaat aataattata ataactgcat
    16321 gtagttattt aatcggtatg ggaggaggtc cattagctgc tataaaaatg ggtgagcaaa
    16381 acaatgatga ggcagagaaa ataatgagta acagtttttc tgtattggtg attttagcaa
    16441 tacttttgac tataggtttt aagattggaa aagagccttt actttggatg tttggagcaa
    16501 gtgaatctac aattggatat tcaatggact atttaaatat ttatttaata ggtacagtgt
    16561 ttgttcagat ttcaatgggt atgaatactt ttataaatac ccaagggttt gctacaactg
    16621 gaatgatgac agttgctata ggtgctttaa taaacataat acttgacccg atttttatat
    16681 ttggctttaa tatgggtgta aaaggagctg cattagctac tataattgca caaggcgttt
    16741 ctgctatttg ggtacttatg tttctctttg gaaagaaaag tatacttaaa ataaaaaaga
    16801 agtatatgat accaaaagca agtatcatct taccagtgct tggcttggga atctctccat
    16861 ttataatgca atctacagaa agtttagtac ttatagcctt aaatagtaaa ttacaaatgt
    16921 atggaggaga tttagcagta ggttcaatgg ccataatgag cagtataatg caaattctta
    16981 tgcttcctaa tatgggggtt acacaaggag cacaaccaat aataagttat aattatggtt
    17041 caggtcaact tgatagagta aaaaagacat ttaagttgtg tttattgtca tgttttacat
    17101 attctactat tttatggctt ttattaatga tatttcctgc attttttgtt agtatattta
    17161 ataagaatcc gcagttactt agtatgacta gttggagtat aaaaatatat tttgctggtg
    17221 cttttatgtt tgggattcaa atagcttgtc aacagacatt tttagcactt ggaaaagcaa
    17281 caatatcttt ggttttagct ctacttagaa agattgtact tttaatacct ttaatattta
    17341 tacttccaac attctttaat gaaaaacttt ttgcagtaat tttagcagag ccagttgcag
    17401 atataacagc cgctactata acagcaattt ctttctttat attttataaa tgttttctat
    17461 caaaacctaa agcaataaaa gaataag
//
LOCUS       DAYAPA010000086        17229 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.086, whole genome shotgun sequence.
ACCESSION   DAYAPA010000086 DAYAPA010000000
VERSION     DAYAPA010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17229
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.086"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            29..565
                     /locus_tag="ACGI59_002623"
     CDS             29..565
                     /locus_tag="ACGI59_002623"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9740405.1"
                     /translation="MAKFKKKSEEVEAFKWILGSPNTPIWFYQAFEKGTIWLDESLNS
                     MNRKGEVKKTICIKYKNGVIRATNGDFIIKDSEGKIYSCTFSEFEKLYENLERGHRYV
                     DEDNNKKNKLELSAKLELDTTDFEENIKSATKEIETLNEVVDRLEKKLNRIFAKENKV
                     DIDSIVKQLEERLRESIE"
     gene            584..856
                     /locus_tag="ACGI59_002624"
     CDS             584..856
                     /locus_tag="ACGI59_002624"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9740406.1"
                     /translation="MFKKKYIKKPSKISVRNIMAFIITVVGIALGVFIGINIIMAHVL
                     GIANMVDNNTFTCVRLVYGLAGVISGYLIGKAIYLIASLISHIIYE"
     gene            907..1134
                     /locus_tag="ACGI59_002625"
     CDS             907..1134
                     /locus_tag="ACGI59_002625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9740407.1"
                     /translation="MKEYVIWFKSGNCVSGITNEDVTDKLMKDFMEADSDCRYLKGYL
                     DEDGTTVIDLSQIEAISINNCSENNIGFSKS"
     gene            1180..1752
                     /locus_tag="ACGI59_002626"
     CDS             1180..1752
                     /locus_tag="ACGI59_002626"
                     /inference="COORDINATES: protein motif:HMM:NF018515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage scaffolding protein"
                     /protein_id="HFG9740408.1"
                     /translation="MKKGELIALGLSEEDAKKVEAESLKELENYINKIEYEKVKEELK
                     ASKEAIEGFKDGMTKEQIEELKKGYETKLTAKDEEYQKKLKEKEQKEFDMALENELIK
                     LNVHSTKAAKAELDLEKIKYENGTFTGLKEQTDTWLTQKSFLIKTGETKINYSPDNGN
                     KNTLSRAENIAKEKNEEGSKNPYADAWSIK"
     gene            1768..2148
                     /locus_tag="ACGI59_002627"
     CDS             1768..2148
                     /locus_tag="ACGI59_002627"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9740409.1"
                     /translation="MYFKEVNFDNTPEFLASQHYINFSKTALDTDVVADENGKKYVLA
                     GSLLGESGKVVKITRGGSSGSYTYTLSEDPVGIVFSTVDVTYGPQPVASMVEGYVIIE
                     RLQGEYVKEAIETIKTKLPNIKFM"
     gene            2160..3179
                     /locus_tag="ACGI59_002628"
     CDS             2160..3179
                     /locus_tag="ACGI59_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009530869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFG9740410.1"
                     /translation="MARVEELLSVQELINYTKTRKLKETMGDLLFPTQKIEGLEIKMI
                     KGASNLPVSASVHAFDTEAEIASREGANLSIAELALVKRKIKLDEKDIIVLEEPRNSQ
                     EETQMINQIFNDVDNLVSSVNTRIEAMRMEVLSTGELNINENGVKASLKYGTPTNHKE
                     TKTWSSGTPDILGDIYNMTDKIVVDTGFTPTRSLTSKTILNIILRDEKLRKAIFGVNS
                     DKLLTLNELNTFLVSQSLPPIFTYDERYRVQGKDGKYTTKRFLDENKFILMPDGKMGD
                     TFFGLTAEELELRKNPAIDISSVGNIIVEQYSTADPVAKWIKAVATALPSFPYADQVF
                     MGTIN"
     gene            3190..3534
                     /locus_tag="ACGI59_002629"
     CDS             3190..3534
                     /locus_tag="ACGI59_002629"
                     /inference="COORDINATES: protein motif:HMM:NF016990.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HFG9740411.1"
                     /translation="MEVERLKKLLGISKEDYSKEMILEFILEDVEEIVKNYCNVSVIP
                     EGLNSTVLRMAIDMYKNENLGSEDIALGSISSISEGDTSVSYRSSASEFKESLLKDYK
                     SQLNRYRKIRWK"
     gene            3537..3896
                     /locus_tag="ACGI59_002630"
     CDS             3537..3896
                     /locus_tag="ACGI59_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9740412.1"
                     /translation="MDKTRKAIEMLYRDKCTIIEYQPVKDPVTKRTNNKEVIVLENQP
                     CKLSYKNINSTEEGKVAKLAQTIKLFISPNISVKAGSKLIITTQNNITREYVRSGESA
                     IYPNHQEIILELFKDKA"
     gene            3896..4321
                     /locus_tag="ACGI59_002631"
     CDS             3896..4321
                     /locus_tag="ACGI59_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HFG9740413.1"
                     /translation="MARWGSVDFREFKRVCKKMEKLTKIDLDKFCKDAARELAARLLG
                     KVIRRTPVDTGFLRQGWNGVAYARSLPVYKQGNNYIIEVVNPTEYASYVNFGHRTKDG
                     KGWVKGQHFLTISEMELQSQVDKIIERKLLILLKGVFDA"
     gene            4314..4751
                     /locus_tag="ACGI59_002632"
     CDS             4314..4751
                     /locus_tag="ACGI59_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6838 family protein"
                     /protein_id="HFG9740414.1"
                     /translation="MLNNIIDGISIKLDKSFGNEYTIYSEDVEQGINEPCFFIVPLNP
                     SKVSYPSGRTLKKNSFDVHYFPRSKDKSFEINEVAEMLLEELEYIEIDGDLVRGTNMN
                     FEIIDNVLHFFVDYNYFTIKSNDTEKMNDVELFGGLKRGDNFE"
     gene            4735..4920
                     /locus_tag="ACGI59_002633"
     CDS             4735..4920
                     /locus_tag="ACGI59_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9740415.1"
                     /translation="MIILNKTLSKEDDYKFTKEQIVNSKKYVNRKDLLNAILKENELY
                     SFSEVEEIINNFMKGVS"
     gene            4921..6231
                     /locus_tag="ACGI59_002634"
     CDS             4921..6231
                     /locus_tag="ACGI59_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath family protein"
                     /protein_id="HFG9740416.1"
                     /translation="MALGGGTFVTQNKVLPGAYINFVSATRATSSLSDRGIVAMPLEL
                     DWGIDEEIFTVTSDDFEKYSTKYFGYDYTHEKLKGLRDLFKNIRLGYFYKLNKGVKAS
                     CSIATAKYSGTRGNDLKVIVTTNIDDNTKFDVVTLLDNKKVDTQIAKVITDLQDNDYI
                     TWKKDTTLEASAGLVFTGGTNGESVTGAEYQAFLDKIESYSFNALGSLATTAEIKSLF
                     VEFTKRMRDKVGAKFQTVLYKKNDADYEGVVSVENKVKDTGLLESSLVYWTTGAIAGC
                     DINKSNTNKKYDGEFDVDVNYTQIQLEEALKSGKFIFHKVGDEVHVLEDINTFVSFTD
                     DKNDDFSSNQSVRVLDQIANDIATLFNEKYLGKVPNDKAGRISFWNDVVKHHKELENI
                     RAIEDFKTDDVSVELGNDKKTVIVSDAVKVINAMSKLYMTVSVS"
     gene            6252..6728
                     /locus_tag="ACGI59_002635"
     CDS             6252..6728
                     /locus_tag="ACGI59_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFG9740417.1"
                     /translation="MAKNITMKSKDAISASLAECYVTIEGKRYLLMQCIKVEAKFKKN
                     KSKIPILGQTGKGNKTTGWEGTGSATVHYNTSIFRKLMLRYKETGEDIYFDMQITNED
                     PTSSVGRQTIILKDCNMDEVTLAKFDADSEYLDEDIDFTFDDWDMPEEFKILLGMI"
     gene            6797..7237
                     /locus_tag="ACGI59_002636"
     CDS             6797..7237
                     /locus_tag="ACGI59_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFG9740418.1"
                     /translation="MSNLSAFLSQNAIKNENVMYVASDRFLDEGGKPVEWELRVLSSE
                     EDEVLRRNCTKRVKVIGNNGKPTGQFTSEIDYNSYVAELCVASTVFPDLKDAELQNSY
                     GVMGEAQLLKTMLTAGEYVNYTVKVNEVNGFDTTFEDKVEEAKN"
     gene            7700..8260
                     /locus_tag="ACGI59_002637"
     CDS             7700..8260
                     /locus_tag="ACGI59_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein L7/L12"
                     /protein_id="HFG9740419.1"
                     /translation="MFCSNCGAEITGIGKFCSNCGAAVENEIIEDNNIKSNDLIIDAN
                     GIEVNMTEIYRKYRKEKVNAIKNVMEISGLNIKEAKKIVDSSFEELKNNFIDDTMSNS
                     EKEKITNNQNRKNNIEKAQQESVACCPKCGSTSLTAQKKGFGIGKAVVGASLTGGIGL
                     VAGNLGAKKVRVTCLNCGKQFWAGKK"
     gene            8338..10704
                     /locus_tag="ACGI59_002638"
     CDS             8338..10704
                     /locus_tag="ACGI59_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFG9740420.1"
                     /translation="MATIQTSIRIFDGMTPAFRHMTNAMNIVLSSFEQLQRTSSNAID
                     ANSIRTAREELARAEAGFDRLEQQIREADEQQKRFNDDVRKGASSTDKLVENAKRLVA
                     TYIGLRSVGGLINLSDQMTSTNARLAMINDGQQSDGGLNKMIFQSAERARASYLDTAK
                     IVSRVGMNAGKAFSSTKEIVAFAEQLNKKFVIAGATTEETNSALLQLTQGLGSGVLRG
                     EELNAVFESAPNIIQSIADYLEVDIGKIRGMASEGMLTADIVKNSLLAAAEQTNSEFE
                     KMPYTFSQIWTSIKNNAIMIFGVIQKKIEQAMSSRGFRTFIDNFIDSLYVLGAVAFSI
                     FNGIINILGSPFFQAFVNAIIVGVSLIVQVLGWVITQALNITNVFAQNWSIIAPIVLG
                     VAAAMLVYNNALLLSIANKVKDIALSAKTLAMNFAHIIAESYRAAALVASTIAQDGLN
                     AAMAACPITWILYGIIAIVVAFFVAIAVINKFAGTSYSAIGIVAGALSGLTAFIINSV
                     FFWINVFISFAEFFTNVLDHPVYSVKKLFFNLATAVLNNLISMTKGCDEFATNLANSI
                     IDGINGALKAWNKFVDVLNKFGGLGDKLGLGKADMVGHTKSITSTLQKAKGDLNKWLG
                     AEPKGYKSFKQLEYNNVGDWAKNGYAFGQNLENKIKDTFDISKIADKAKKDLGLDDLW
                     DDKYGLGDGIGSAGLNSPLGDAAKGAKDTAGNTAKMAKTMDKSQEDLKYLRDIAEQET
                     INRFTGVNIKIDMNNTNNINKDADLDGIVNVLTEKLNDAMVVSAEGVV"
     gene            10720..11415
                     /locus_tag="ACGI59_002639"
     CDS             10720..11415
                     /locus_tag="ACGI59_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFG9740421.1"
                     /translation="MAYDFYLDGVQLPIAPGKLEIKITNKNKTVDLINTGEVNILKKE
                     GLSEISFEAEFTHNKLPFYRGAFKDVQFFLSKLELLKTDCKPFQFIVSRELGNKVLFN
                     TNIKVSLEEYAISEDAENGSDTKVAIKLKQYRDYSTKKLVLAPPKNETGRPSVKIEPK
                     RVDSVNATNTKTKTYTVKAGDSLWSICQKQLGNGSLYKKVYELNKSMMDKANKGKNLS
                     KYTIYKGQVLKLG"
     gene            11408..13378
                     /locus_tag="ACGI59_002640"
     CDS             11408..13378
                     /locus_tag="ACGI59_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFG9740422.1"
                     /translation="MVDELVLANDRDVRLVIAHWEDFYEPAVIDGITWEIERRGTPSK
                     LEFTIVMDDILQFCEGNSVRLYYKGVGIFYGYIFQKKRDKENHIKIIAYDQLRYFKNK
                     DTYVYSNKTASELIKMLAKDFNLKYNVIEDTKYKISRIEENKTLFDMILTALDDTLRE
                     KKEMFTLYDDFGRITLKNVASMKLDTVMNNDVIEDFDYNSSIDSDTYTKIKLVRDNEE
                     TGKRDVYIAQDSTHMRSWGILQLFDTVDKNMSEAEIKQKCDILLKLYNKKTKSLSLKN
                     VLGDIRVRAGCLVPVFLSLGDIDLQNYMLVEKVKHTFENNSHFMDLTLVDGDEFASYS
                     SSSYSSGNTNNKNEKQNGPAQSTTSKEDTDMANKINKLLKGKLSNTGNIFVKYSNAYK
                     VNPALMAAISMHESARGTSNIANTKNNFFGMKKNGDYMSFSSVDEGIKRGISNLSRNY
                     IHIGRKTLESIRNKYSSSSDKEWVKCVGAFYKQITGSTYNSNSAGTGVGSNEEAEKNL
                     KDLTYQVQNNNSNTSTNNNNKVSKVIQEAKNQLGKPYKWGGNGPKSFDCSGLMVWAFK
                     RGAGINLKRVSADQSKDSRGKLLCNINDVKAGDLVFFAYNKGKGNVHHVGLYIGNDQY
                     IHAPQTGDVVKISSLSGRQKKKHDFARARRFF"
     gene            13392..13652
                     /locus_tag="ACGI59_002641"
     CDS             13392..13652
                     /locus_tag="ACGI59_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2577 domain-containing protein"
                     /protein_id="HFG9740423.1"
                     /translation="MSQELLQIIKKAAMDAVETSNPMQIAFGTIESVNPLIVKIEQKA
                     SFEEFFLIQTETFKRYTDKKIGDKLVLIRMQGGQQYLILDRM"
     gene            13657..14076
                     /locus_tag="ACGI59_002642"
     CDS             13657..14076
                     /locus_tag="ACGI59_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HFG9740424.1"
                     /translation="MLPSDNLDYDIEDVSIINFDVRQEPSKTFKLNIEKSKIDGICDD
                     VEALKQTIFLILNTERYEHLIYSWNYGVELNDLIGEPISFVIPELERRIKEALIQDDR
                     VENVDNFEFQNVKGKVHCKFTVHSKYGNIKAEKVVSV"
     gene            14077..15126
                     /locus_tag="ACGI59_002643"
     CDS             14077..15126
                     /locus_tag="ACGI59_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFG9740425.1"
                     /translation="MFELMTFENIIKRMLDSVPDTFDKREGSIIYNALAPVAIELTET
                     YIAMDELLDQTFVDTASYYYLEKRCKERGITPLSATNTIAKGVFNIDIPLDSRFNLGE
                     YNYIAIERISEKTYKMKCETAGPIFELGKLIPIEYIDGLETAELTEILINGEDEESED
                     SLRQRYYDSLNSQSFGGNMQNYKDEVNKIQDVGGVKVYPVWDGGGTVKLVIINSNFKV
                     PSEDLVNLVQEEIDPIGHQGQGLGLAPIGHKVTVTGVVSTTINISAEITYKNGYTWEN
                     IKSIAEEAIDDYLNELNMSWEDEENLIVRISQIETRLLSIDGVLDIANTMINEVKSNL
                     TINSNSIVVRGEVVG"
     gene            15119..15736
                     /locus_tag="ACGI59_002644"
     CDS             15119..15736
                     /locus_tag="ACGI59_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HFG9740426.1"
                     /translation="MDKEINLINYLPQILQDKEEYIKVFNVGNKEIKILHDKLKDLSN
                     DQFLEDLTISGIKRWEKIMSITPKSNESLEDRRFRIFSKYISKLPYSERFLRNWLDSI
                     VGEGNYELTINNATYNIHLESDARNQDWFEEVHSFVSNIKPCNMTLDYTRVLISKDNY
                     MNFGITTLMGQEITIYPWSPPDIETYGEIDVLTGNGVGYQEITIF"
     gene            15748..16773
                     /locus_tag="ACGI59_002645"
     CDS             15748..16773
                     /locus_tag="ACGI59_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFG9740427.1"
                     /translation="MAIDKSYYTIITDVGKAKIANASVTGNKVGFVKIQLGDGGGSEY
                     TPTESQTALKNVVWEGNIGNTTTDETAPNCIILESLIPSSVGGFMIREIGYLDDENNL
                     IAISKYKECYKPSIEQGAVVDMKVKTVLIVSNVNNIELKIDPTIIFATLKDIQDLETK
                     IGTVNTKIDTTKTELTNNLETAKTELNTRIDTENEKQNIKIDQLIAGGSNVASTQTIT
                     IDDWVEDAENGFKATVTHSLLTQRIVVNIIDATTKENVVTNFKIIDDNTIEIRSETRS
                     ELNVYVINGNAETHFINATVDDNRVSEMTTYSSKKIEDRLVNIEEKLSGGLSNIATSV
                     NELITYC"
     gene            16788..>17229
                     /locus_tag="ACGI59_002646"
     CDS             16788..>17229
                     /locus_tag="ACGI59_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine rich domain-containing protein"
                     /protein_id="HFG9740428.1"
                     /translation="MQTEWNFGYNGSPQSVILKPGKYKFECWGSSGGINNSSWHTDAK
                     GGYSKGEITLKKQTTLYVYVGESGFASSSTSNNTKSGFNGGGKGYLNQQVMGTYYSMY
                     GGGATDIRLVGGAWDNEQGLLSRIIVAGGGGGSYSPYTGGAGGGL"
BASE COUNT         6901 a         1879 c         3412 g         5037 t
ORIGIN      
        1 attaaaaatt taaaggggga taaataatat ggctaaattt aaaaagaaat cagaggaagt
       61 agaagctttt aaatggatat taggaagtcc taatactcct atatggtttt atcaagcttt
      121 tgagaaggga actatatggc ttgatgaatc tttaaactct atgaatcgta aaggtgaagt
      181 aaagaaaact atctgtataa aatataaaaa tggagttatt agggcaacta atggagattt
      241 tattataaaa gatagtgaag gcaagattta ttcatgtaca tttagtgaat ttgagaagtt
      301 atatgaaaat ttagaaagag gacatagata tgttgatgaa gataacaaca agaaaaataa
      361 attagaactt tcagctaaat tggaactaga tacaactgat tttgaggaaa atataaaaag
      421 tgctacaaaa gaaattgaaa cactcaatga agtagtagat agattagaga aaaaattaaa
      481 tagaatattt gcaaaagaaa ataaagttga tatagatagt attgtaaaac aattagagga
      541 acgtctaaga gaaagtattg aataagtttt gagggatgga aatatgttta aaaagaaata
      601 tattaaaaag ccaagcaaaa taagtgttag aaatattatg gcatttatta ttacagttgt
      661 tggtatagct ttaggtgttt ttataggtat caacataatc atggctcatg ttttaggtat
      721 agctaacatg gtagataata atacttttac atgtgttaga ttagtttatg gcttagcagg
      781 agttataagt gggtatttga taggaaaagc aatatacctt atagcttcgc taataagcca
      841 tattatttat gaataatttg ttcaaagatt ttttattttg taagaaatga aaggagggtt
      901 gtattattga aagagtatgt aatttggttt aagagtggaa attgtgtatc tggaataaca
      961 aacgaagatg ttactgataa gttaatgaaa gattttatgg aagctgactc agattgtagg
     1021 tatttgaaag ggtatttaga tgaagatgga acaactgtaa tagatttatc acaaatagaa
     1081 gctatatcaa taaataattg tagtgagaat aatattggtt ttagtaagtc ctaacaaggg
     1141 cttttttatt ttctaaaaat gaaaggagaa ttaaataaaa tgaaaaaagg tgaactaatt
     1201 gcactaggtc ttagtgaaga agatgcaaag aaagtagaag ctgaatcatt aaaagaatta
     1261 gaaaattata ttaataaaat tgagtatgaa aaggtaaaag aggaactaaa agcctctaaa
     1321 gaagcaattg aaggttttaa ggatggaatg acaaaagagc agattgaaga gcttaaaaaa
     1381 ggctatgaga ctaaattaac tgcaaaagat gaagaatatc aaaagaaatt aaaggaaaaa
     1441 gaacaaaaag agtttgatat ggcgttagaa aatgaactta ttaaacttaa tgttcatagc
     1501 actaaagcag caaaagcaga acttgattta gaaaaaataa aatatgaaaa tggtactttt
     1561 acaggactaa aggaacagac tgatacttgg ttaactcaaa aatctttctt aataaaaaca
     1621 ggagagacta agataaatta cagccccgat aatggcaata aaaatacatt aagtagagct
     1681 gaaaatattg ctaaagaaaa gaatgaggaa ggttctaaaa atccatatgc tgacgcatgg
     1741 agtataaaat aaaaaggagg ataaagtatg tattttaaag aggtaaattt tgataacaca
     1801 cccgagtttc tagcttctca acactatatt aacttttcaa aaacagcatt agatacagat
     1861 gtagtggctg atgaaaatgg aaagaaatat gtgttagcag gtagtttatt aggtgagagt
     1921 ggcaaagtgg taaagataac cagaggaggt tcttcgggta gttatacata cacattatca
     1981 gaagaccctg taggaatagt tttttcaact gtagatgtta cttatggacc acaaccagtt
     2041 gcatcaatgg tggaaggcta tgtaataatc gaaagattgc aaggtgagta tgtaaaagaa
     2101 gctatagaaa ctataaagac aaaattacca aatattaaat ttatgtagga ggatgaaata
     2161 tggcaagagt agaagaatta ttgtcagttc aagagctgat aaactataca aagactagaa
     2221 aattaaaaga aacaatggga gatttattat ttccaactca aaagatagaa ggacttgaaa
     2281 taaagatgat aaaaggtgca tctaatcttc cagtatcagc aagtgttcat gcttttgata
     2341 cagaagcaga gattgcatca agagaaggtg ctaatttaag tattgctgaa cttgcacttg
     2401 tgaaaagaaa aattaaacta gatgaaaaag atataattgt acttgaagag ccaagaaact
     2461 cacaagaaga aactcaaatg ataaatcaaa tatttaatga tgttgataac cttgtatcaa
     2521 gtgtaaatac tagaatagaa gcaatgagaa tggaagtttt atcaactgga gaacttaata
     2581 taaatgaaaa tggagttaaa gcttctttaa aatatggaac tccaacaaat cataaagaaa
     2641 caaaaacttg gtctagtgga acaccagata tattaggaga tatttataat atgactgata
     2701 aaatagttgt tgatactgga tttactccaa caaggtcatt aacttctaaa actattttaa
     2761 atataatctt aagagatgaa aaactaagaa aagctatatt tggtgtaaat agtgataaac
     2821 tacttacttt aaatgaatta aatacatttt tagtttctca atctcttcct cctattttta
     2881 cttatgatga aagatacaga gtacaaggca aggatggtaa gtacacaaca aagagattct
     2941 tagatgaaaa taagtttatt cttatgcctg acggcaagat gggagatact ttctttgggc
     3001 taacagcaga ggaattagaa cttagaaaaa acccagcaat agatattagt tcagttggaa
     3061 atataattgt agaacaatac tctactgctg acccagttgc taagtggata aaagcagttg
     3121 caacagcatt acctagtttt ccttatgctg accaagtgtt tatgggtaca ataaattaga
     3181 ggtgttgata tggaagttga aagactaaaa aaacttttag gaatcagtaa ggaagattat
     3241 tcaaaagaga tgatattaga gtttatatta gaagatgtgg aggaaatagt taaaaattat
     3301 tgtaatgtat ctgttattcc cgaagggcta aatagtactg ttttaaggat ggctatagat
     3361 atgtataaaa atgagaatct aggaagtgaa gatattgcac taggttctat ttcttctata
     3421 tcagaaggtg atacatcagt ttcatacaga agttcagcta gtgaatttaa ggaatcgtta
     3481 ctcaaagatt ataagtcaca attaaacaga tacagaaaaa ttaggtggaa ataatgatgg
     3541 ataagactag aaaagcaata gaaatgttat acagagataa atgtactata attgagtatc
     3601 agccagtcaa agaccctgtg acaaaacgaa ctaacaataa agaagtgatt gtattagaaa
     3661 atcaaccttg taagctttca tataaaaaca ttaattctac agaagaagga aaagtagcta
     3721 aacttgcaca aaccatcaaa ctctttatat ctccaaacat aagcgttaaa gcaggttcaa
     3781 agcttattat aactactcaa aataacataa caagagagta tgtaagaagt ggagaatcag
     3841 ctatatatcc aaatcatcaa gaaataatac ttgagttatt taaggataaa gcataatggc
     3901 tagatggggc agtgttgatt ttagagagtt taaaagagtt tgtaaaaaga tggagaagct
     3961 tacaaagatt gatttagata agttttgcaa ggatgcagca agagaattag cagcacgatt
     4021 acttgggaaa gtaattagaa gaacaccagt tgatacagga ttcttacgac aaggatggaa
     4081 tggagtggct tatgctaggt cgcttcctgt gtataaacaa ggaaataatt atattataga
     4141 agttgttaat ccgactgaat atgcaagtta tgtaaatttc gggcatagaa ctaaagatgg
     4201 taagggatgg gttaaaggac aacatttctt aacaatttca gagatggaac tacaaagtca
     4261 agttgataag attatagaga gaaagttatt aatcttgttg aaaggagtat ttgatgctta
     4321 ataatataat tgatggaata tctattaaat tagataaatc atttggaaat gaatatacaa
     4381 tttatagtga agatgtggag caaggtataa atgaaccttg tttttttatt gttcctttaa
     4441 atccaagcaa agtatcatat ccaagtggca ggacattaaa aaagaactct tttgatgtac
     4501 attattttcc tcgttcaaaa gataagagtt ttgaaataaa tgaggtagct gagatgctac
     4561 ttgaggaatt agagtatata gaaattgatg gagatttagt tagaggcaca aatatgaatt
     4621 ttgaaattat agataatgtt cttcatttct tcgttgatta taactacttc actataaaaa
     4681 gtaatgatac agaaaaaatg aatgatgttg aattatttgg tggtttgaag agaggtgata
     4741 attttgaata aaacattaag caaagaagat gactacaagt ttactaagga gcaaatagtt
     4801 aattctaaga agtatgtaaa tagaaaagac ttattaaatg caattttaaa agaaaatgag
     4861 ttatattcct tctcagaagt agaggaaata ataaataatt ttatgaaagg agtgagttag
     4921 atggctttag gtggaggaac atttgtaaca caaaataagg tcctacctgg tgcatatata
     4981 aattttgtaa gtgctacaag ggcaaccagt tcattatcgg atagaggtat tgttgcaatg
     5041 cctttagagt tagattgggg catagatgaa gaaattttca cagtaaccag tgatgatttt
     5101 gagaagtatt caactaagta ttttggatat gattatactc atgagaagct gaaaggtttg
     5161 agagatttat tcaaaaatat aaggttggga tatttttata aattgaataa aggtgttaaa
     5221 gctagttgca gtattgctac agctaagtac tcaggtacta gaggtaatga tttaaaagtt
     5281 atagttacaa caaacattga tgataacact aagtttgatg ttgtaacact tttggataat
     5341 aagaaagtag atacccaaat agcaaaagtt attacagact tacaggacaa tgactatatt
     5401 acttggaaga aggatacaac actagaagca agtgcagggc ttgtatttac tggtggaact
     5461 aatggcgaat cagtcacagg agcagagtat caagctttct tggataaaat agaaagctac
     5521 tcatttaatg cactaggcag tttggctaca acagcagaaa ttaaaagttt atttgtagag
     5581 tttactaaaa gaatgagaga taaagtagga gctaagtttc aaactgtact atataaaaag
     5641 aatgatgcag attacgaagg tgtagtatct gtagaaaata aagttaaaga tactgggtta
     5701 ttagaatcta gtttagttta ttggactact ggagctatag caggatgcga tataaataaa
     5761 tctaatacta ataaaaagta tgatggtgag tttgatgttg atgtaaatta cacacaaata
     5821 caacttgaag aagcactaaa gagtggtaaa tttatatttc ataaagttgg tgatgaagtt
     5881 catgtgttag aggacataaa tacttttgta tcatttacag atgataaaaa tgacgatttt
     5941 tcaagtaacc aaagtgttag agtacttgac caaattgcta atgatattgc aactttattt
     6001 aatgaaaagt atttaggtaa agttccgaat gataaggcag gaagaataag tttctggaat
     6061 gatgttgtta aacaccataa agaattagag aatataaggg caatagaaga ttttaaaact
     6121 gatgatgtta gtgtggagct tggaaatgac aagaaaacag ttatagtgag tgatgcggtt
     6181 aaggttataa atgctatgag taagctttat atgactgttt cagttagtta attaggaagg
     6241 agaatattag aatggctaaa aatattacta tgaagtcaaa agatgcaata agtgcatctt
     6301 tggcagaatg ctatgttacc attgaaggta aaagatacct tcttatgcag tgtatcaagg
     6361 tagaggctaa atttaagaag aataaaagta agataccaat tttagggcaa acaggaaaag
     6421 gaaacaaaac tactggatgg gaaggcacag gaagtgcaac tgttcattat aatacttcta
     6481 tctttagaaa acttatgctt agatataagg aaactggaga agatatatat ttcgatatgc
     6541 aaattactaa tgaagaccca acaagttcag taggtcgtca aactattata ttaaaagatt
     6601 gcaatatgga tgaggttaca ttagctaaat ttgatgcaga tagtgaatat ttagatgagg
     6661 atatagattt tacttttgat gattgggata tgccagaaga atttaaaatt ttattaggta
     6721 tgatatagaa tacacattta taaattatag atgtgtgttt tttatgtaaa aattaaaata
     6781 aaaggagaat taagaaatga gtaatttaag tgctttttta agtcaaaatg caataaaaaa
     6841 tgagaatgta atgtatgtgg caagcgatag atttttagac gaaggaggaa agccagttga
     6901 atgggaatta agagttttat cttctgagga agatgaagta ttaagaagaa actgtaccaa
     6961 aagagtgaaa gtgattggca ataatgggaa gccaacaggt caattcacaa gtgaaattga
     7021 ctacaatagt tatgtagcag agttatgtgt agcatctaca gtatttccag atttaaagga
     7081 tgccgaactc caaaatagtt atggagtgat gggagaagct cagttattaa aaacaatgct
     7141 tacagcaggt gagtatgtaa attatacagt aaaagttaac gaagttaatg gatttgatac
     7201 aacatttgaa gataaagtag aagaagcaaa aaactaatta ggggcggtga ttttgatgct
     7261 agcatcactc attattgtat ccaaaaatta aagtggaaac ctagtgaata tatgaattta
     7321 gaagttaatg agagagcatt agcagccgcc tcaatacttg ttaagataga ggatgaagag
     7381 gaagcaatga aagaatctga aagagagaaa aagagaggaa ggagaaggta ggcagggatt
     7441 aaatgaataa agatatagaa cttacagaaa aagatttata ttgtatcact agacatatta
     7501 aagtgtatat gttcaaaaaa atgatgaagt gataaaaaga gaagataatc catgttgtag
     7561 atatttatca ctgcaccaca tttaagaaat tatctaaaat tacaggtctt aaaatgggtt
     7621 ttggagtcaa actataatgt aaaaattttt atggatatag tataatattt ataaaaatat
     7681 tgtacagggg ggaattatta tgttttgctc aaattgtggt gcagaaatca caggtatagg
     7741 caagttttgt tcgaattgtg gggctgctgt agaaaatgag attattgaag ataataatat
     7801 taaatcaaat gatttaatca ttgatgctaa tggaatagaa gtaaatatga ctgagattta
     7861 tagaaaatat agaaaagaaa aagtaaatgc aataaaaaat gtaatggaaa taagcggttt
     7921 gaatataaag gaagcaaaaa aaatagtgga ttcttccttt gaagagttaa aaaacaactt
     7981 tattgatgat actatgagta attcagaaaa ggaaaaaata acaaacaatc aaaatagaaa
     8041 aaataatatt gaaaaagctc aacaagaatc agttgcttgt tgtcctaagt gtggttcaac
     8101 atccttgaca gctcaaaaaa agggttttgg tataggaaaa gcagtagtag gagcgagttt
     8161 aactggtgga ataggtttag tagctggaaa tttaggagca aagaaagtta gagtaacatg
     8221 tttgaactgt ggcaagcaat tttgggcagg caaaaaatag atgtattcat aataaaaaca
     8281 cttactaaat tagtaagtgt ttttattatc taaattaaca gaaaggagag cagaaaaatg
     8341 gcaactatac aaacatctat ccgaattttc gacggaatga cacctgcttt tagacacatg
     8401 actaatgcta tgaatattgt attaagttca ttcgagcaat tacaaagaac atctagcaat
     8461 gctatagatg ctaatagtat tagaacagct agagaagagt tagcaagggc agaagctgga
     8521 tttgatagat tagaacaaca aataagagaa gctgatgaac aacaaaaaag atttaatgat
     8581 gatgttagaa aaggagcaag tagtacagat aagttagtag aaaatgctaa aagattagta
     8641 gcaacttata ttggattgag aagtgttggt ggtttgatta atttaagtga ccaaatgacg
     8701 agtactaatg cgagactagc tatgataaat gacgggcaac aatctgatgg aggactcaac
     8761 aaaatgatat ttcaatcagc ggaaagagca agagcatctt atttagatac tgcaaaaatt
     8821 gtaagcagag tgggcatgaa tgcaggcaaa gcatttagta gtacaaaaga aatagtagca
     8881 ttcgcggaac aacttaacaa gaaatttgta attgcaggag caacaacaga agaaactaat
     8941 tcagcacttt tacaacttac acaaggttta ggaagtggtg tgttaagagg cgaggaacta
     9001 aatgctgtgt ttgagtcagc acctaatatt attcagtcta ttgccgacta tctcgaggtc
     9061 gatataggga agataagagg tatggcaagt gaaggaatgt taacagcaga tattgtaaaa
     9121 aactcattac ttgcagcagc agagcagacc aattcagagt ttgaaaaaat gccttacaca
     9181 ttttctcaaa tttggacttc aattaaaaat aatgcaatca tgatatttgg tgttatacag
     9241 aaaaaaatag aacaagctat gtctagtagg ggttttcgaa cctttataga taattttata
     9301 gattcgttat atgttttagg agcagttgca tttagtattt ttaatggaat tataaatata
     9361 ttaggaagtc cattttttca agcatttgta aatgctatta ttgtaggtgt tagtctgata
     9421 gtgcaagtgc ttggctgggt aataacacaa gcattaaata ttactaatgt atttgctcag
     9481 aactggagta ttattgcacc aatagtactt ggagttgcag ctgctatgtt agtatataac
     9541 aatgcactat tacttagtat agctaataaa gtaaaagata ttgcgttatc tgctaaaact
     9601 ttagcgatga attttgctca tataatagca gagtcttata gagcagcagc attagtagca
     9661 agcacaattg cacaggacgg attgaacgca gcaatggcag cttgccctat cacttggatt
     9721 ttatatggaa ttatagccat agttgttgcc ttttttgtag ctatagctgt aataaataaa
     9781 ttcgcaggaa catcttattc agcaattgga attgttgcag gagcattatc gggtttaaca
     9841 gcatttatta taaatagtgt atttttctgg attaatgttt ttatatcatt tgctgagttt
     9901 tttacaaatg tattagacca tccagtttat tcagtaaaga aattattttt taatttagcc
     9961 acagcagtat taaataatct tatatctatg acaaaaggat gtgatgaatt tgctactaat
    10021 ttagcaaact ctattataga tggaattaat ggggcattaa aagcttggaa taaatttgta
    10081 gatgtcttaa ataaatttgg aggacttgga gataagttag gacttggaaa agctgatatg
    10141 gttggtcata caaagtctat tacaagtaca ttgcaaaaag ctaaaggaga cttaaataaa
    10201 tggcttggag cagagccaaa aggttataaa tcctttaaac aattagagta taacaatgtt
    10261 ggagattggg ctaaaaatgg atatgccttt ggtcaaaact tagaaaataa aataaaggat
    10321 acttttgata ttagtaaaat agctgataag gcaaaaaaag atttaggtct tgacgatttg
    10381 tgggatgata aatatggatt aggtgatgga ataggctcag caggattaaa ctcacctttg
    10441 ggcgatgcag caaaaggagc aaaagatacc gcaggaaata cagctaaaat ggctaaaaca
    10501 atggataaaa gtcaagaaga cttaaaatat cttagagata ttgcagaaca ggaaacaata
    10561 aaccgtttta caggagtcaa cattaaaatt gacatgaaca acacaaataa cataaataaa
    10621 gatgccgact tagatggaat agttaatgta ctaacagaaa aattaaatga cgctatggtt
    10681 gtatctgctg agggagtagt ttagaaagga gggatataaa tggcttatga cttttattta
    10741 gatggagtac aattaccaat cgcacctggc aaattagaaa taaaaataac aaataaaaat
    10801 aaaacagttg atttgataaa tactggagaa gtaaacatac taaaaaaaga agggttatcc
    10861 gagataagtt ttgaagcaga atttacacat aataaattac ctttttatcg tggagctttt
    10921 aaagatgttc aattcttttt aagtaaatta gaactattaa aaacagattg taagccattc
    10981 caatttattg tatcgaggga attaggtaat aaagtactat ttaacactaa tataaaagta
    11041 tctcttgaag agtatgctat ttcagaagat gcagaaaatg gttcagatac aaaagttgca
    11101 ataaagttaa agcaatatag agattactca actaaaaagt tagttcttgc ccctcctaaa
    11161 aatgagactg gtagaccaag cgtaaagata gagccaaaac gagttgattc agtcaatgcc
    11221 acaaacacta aaactaaaac atatacagta aaagcagggg atagcctttg gtcaatttgt
    11281 cagaaacaac ttggtaatgg ttcattatat aagaaagtat acgaactaaa taaatctatg
    11341 atggataagg caaataaggg caaaaactta agtaaataca ctatttacaa agggcaggtg
    11401 ttaaaacttg gttgatgaat tagtgttagc aaatgataga gatgtaaggt tagttattgc
    11461 acattgggaa gatttctacg aacctgctgt cattgatggt atcacatggg aaatagaaag
    11521 acgaggaaca ccttctaagt tagagtttac tatagtcatg gatgatatac tacagttttg
    11581 tgaaggtaat tcagtgagat tatattacaa gggtgtaggc atattctatg gatatatatt
    11641 tcagaagaaa agagataaag aaaatcacat caaaattatt gcatatgacc agttaagata
    11701 ttttaaaaat aaagatactt atgtatatag taataaaaca gcaagcgaac ttataaaaat
    11761 gctcgctaaa gattttaatt taaaatacaa tgtcatagaa gatacaaaat ataaaatatc
    11821 tagaatagaa gaaaacaaaa ctttatttga tatgatttta actgcactag atgatacttt
    11881 aagagaaaaa aaagaaatgt ttaccttgta tgatgacttt ggaagaataa cactaaaaaa
    11941 tgttgcatca atgaaacttg atactgttat gaataatgat gtaatcgagg actttgacta
    12001 taattcttct attgatagtg atacttatac aaaaatcaaa cttgtaagag ataatgaaga
    12061 aacaggaaaa agagatgtgt atattgctca agactcaacg catatgagga gttggggaat
    12121 actacaacta tttgatacag ttgataaaaa tatgagtgaa gcagagataa aacaaaaatg
    12181 tgatatactc ctaaaactat ataataagaa aactaagtca ttaagtttaa aaaatgtgtt
    12241 aggagatatt agagtaagag caggttgttt agtacctgtt tttttgtcgt taggagatat
    12301 tgatttacaa aattatatgt tagttgagaa agtaaaacat acatttgaaa ataacagtca
    12361 ttttatggac ctaactttgg ttgatggaga cgaatttgct tcttattctt catcaagtta
    12421 tagtagtgga aatactaata ataaaaatga gaaacaaaat ggtcctgcac aaagtactac
    12481 aagtaaagaa gatactgata tggctaataa gattaataaa ctacttaaag gtaaattatc
    12541 aaatacagga aatatatttg ttaaatattc aaatgcttat aaagttaatc cagcactcat
    12601 ggctgctata tctatgcacg aatcagctag agggacttca aatattgcaa atactaaaaa
    12661 taatttcttt ggaatgaaaa aaaatggaga ttacatgagt ttttctagtg tagacgaagg
    12721 aataaaaaga ggtataagta atttatcaag aaactatatc catataggac gaaaaacttt
    12781 agaaagcatc agaaataaat attcttctag ttcagacaaa gaatgggtaa aatgtgtagg
    12841 tgcattttat aagcaaataa caggaagtac ttataattct aatagtgcag gcacaggagt
    12901 tggaagtaat gaagaagcag aaaagaattt aaaagattta acttatcaag ttcaaaacaa
    12961 taattctaat acatcaacaa acaataataa taaagtaagt aaagttattc aagaagcaaa
    13021 aaatcaactt ggcaagcctt acaaatgggg tggtaatggt ccaaagagtt ttgactgtag
    13081 tggtcttatg gtgtgggcat ttaaaagagg tgcaggaata aatctcaaaa gagtttcagc
    13141 agaccaatca aaagatagta gaggaaaact attatgtaac ataaatgatg taaaagctgg
    13201 tgatttagta ttctttgcat acaacaaagg aaaaggaaat gtacatcatg ttggactata
    13261 tataggaaat gaccaatata ttcatgctcc acaaactggt gacgtagtaa aaataagtag
    13321 tttaagtggt agacaaaaga aaaagcatga ttttgcaaga gctagaagat tcttttaagt
    13381 gaggtgataa agtgtcacaa gaattattgc aaataattaa gaaggctgca atggatgcag
    13441 tagaaacaag caatccaatg caaattgcat ttggaactat agaaagtgtt aatcctttga
    13501 tagttaagat agaacaaaaa gcatcttttg aagaattttt tctaatacaa acagagactt
    13561 ttaaaagata tacagataaa aaaatagggg ataaattagt cttaattagg atgcaaggag
    13621 gacagcaata tttgatttta gataggatgt gatgaggtgt taccaagcga taatttggat
    13681 tatgacattg aagatgtatc aataattaat tttgatgtaa ggcaagaacc aagtaagacc
    13741 tttaaattaa atatagaaaa atctaagata gatggtattt gtgatgatgt tgaagcatta
    13801 aaacagacca tctttttaat tttaaatact gaaaggtatg agcacctcat ttattcttgg
    13861 aattatggag tcgagttgaa cgaccttatt ggagaaccta tatcctttgt aatccccgaa
    13921 cttgaaagac gaatcaaaga agcactaatt caagatgata gggttgaaaa tgtagataat
    13981 tttgaatttc aaaatgtaaa aggaaaagta cattgtaagt tcacagttca cagtaaatat
    14041 ggaaatataa aagcagagaa ggtggtgagt gtataattgt ttgagttaat gacatttgaa
    14101 aatataatta aaagaatgtt agatagtgta ccagatactt ttgataaaag ggaaggttct
    14161 ataatatata atgctcttgc tcctgttgct atagaactta cagaaacata cattgctatg
    14221 gatgaattac tagaccaaac attcgtagat actgctagtt attattactt ggagaaaaga
    14281 tgtaaagaac gaggaattac accactatct gcaactaata ccattgcaaa aggagtgttt
    14341 aatatagata ttccacttga ttctaggttt aatctaggag aatacaatta tattgcaatt
    14401 gagagaatat ctgaaaaaac atataaaatg aaatgtgaaa ctgctggacc tatatttgag
    14461 ttaggaaaac taatacctat tgaatatata gatggtcttg aaactgctga actaactgaa
    14521 atcttgataa atggagagga tgaagagagt gaggatagtt taagacaaag atattatgat
    14581 agcctaaatt cacagagctt tggtggaaat atgcaaaatt ataaagatga agttaacaaa
    14641 atacaagatg ttggaggagt taaggtttat cctgtgtggg acggtggagg aactgttaag
    14701 ttagtaataa ttaactctaa tttcaaagta ccatcagagg atttagttaa tttagtgcaa
    14761 gaagaaattg acccaattgg acatcaagga caaggcttag gattagcacc aatagggcat
    14821 aaagttactg ttacaggtgt tgtaagtaca actataaata tatcagcaga gataacatac
    14881 aaaaatggct acacttggga gaatataaaa tcaattgcag aagaagcaat agacgactat
    14941 ttaaatgaac ttaacatgag ttgggaagat gaagaaaact taatagtccg tatatctcaa
    15001 attgaaacta gattacttag tattgatgga gtattagaca ttgcaaatac aatgataaat
    15061 gaggttaaat ctaatctaac aataaatagt aacagtatag tagtgagagg tgaggtagtt
    15121 ggataaagag attaatctaa taaattactt accacaaatt ctacaagata aagaagaata
    15181 tataaaagta tttaatgtag gaaataaaga aataaaaata ttacatgata aattaaagga
    15241 cctatcaaat gaccagtttt tagaggacct aactataagt ggtataaaaa gatgggaaaa
    15301 gataatgtct ataactccta aaagtaatga gagtttagaa gatagaaggt ttaggatttt
    15361 tagtaaatat ataagtaaat taccttactc agagagattt ttaaggaatt ggctagatag
    15421 tatagttgga gaaggtaatt atgagttaac tattaataat gctacttata atatacatct
    15481 tgaaagtgat gctagaaatc aagattggtt tgaggaagtt cattcttttg taagtaatat
    15541 taagccatgt aatatgactt tagattacac tagagtgctt ataagcaaag acaattatat
    15601 gaattttggt ataacaaccc taatgggtca agaaataact atataccctt ggagtccacc
    15661 agatatagaa acttatggag aaattgatgt attaactggc aatggagttg gataccaaga
    15721 gataacaata ttttaggagg tgatatattg gctatagata aaagttatta cactataatt
    15781 acagatgtag gaaaagcaaa gatagcaaat gcaagtgtca caggtaataa agtgggattt
    15841 gtaaaaattc aacttggtga tggaggaggg agtgaatata ctccaactga gagtcagaca
    15901 gctctcaaaa acgtggtatg ggaaggcaat attggaaata caactacaga tgaaactgca
    15961 ccaaattgta taatattaga gagtttaata ccatcaagtg taggcggatt tatgataaga
    16021 gaaataggat atttagatga tgaaaataat ttaattgcca tttctaaata caaagagtgt
    16081 tataaacctt ctatagaaca aggtgcagtg gtagacatga aggttaaaac tgtgcttatt
    16141 gtatctaatg taaataatat agaacttaaa attgacccaa caataatctt tgcaacactc
    16201 aaagatatac aagacttaga aactaaaata ggtactgtta atactaaaat agatacaact
    16261 aaaacagaat taacaaataa tttagaaact gctaaaacag agttaaacac tagaattgac
    16321 acagaaaatg agaaacaaaa tattaaaatt gaccaattaa tcgcaggtgg ttctaatgtg
    16381 gcatctactc aaacaataac aattgacgat tgggttgagg atgcagaaaa tggattcaaa
    16441 gcaactgtaa cacatagttt gttaacacag agaatagttg taaatattat agatgctact
    16501 acaaaagaaa atgtagttac aaactttaaa attatagatg ataatactat agaaattaga
    16561 agtgaaacaa ggtcagaatt aaacgtttat gtgataaatg gaaatgcaga aactcatttt
    16621 ataaatgcaa ctgtagatga taacagagtg tctgaaatga ctacttattc gtctaagaaa
    16681 atagaggata gattggttaa tatagaagaa aaattaagtg gtgggttatc taacattgca
    16741 acaagtgtaa atgaattgat aacttattgt taaaggagag tgagaaaatg cagacagaat
    16801 ggaactttgg gtacaatggt tcgccacaaa gtgttatatt gaaacctggc aaatataaat
    16861 ttgaatgctg gggttcttct ggaggtatca acaattcttc ttggcatact gatgctaaag
    16921 gcggatattc taaaggtgaa attacattaa aaaaacaaac tacattatat gtttacgtcg
    16981 gcgaaagtgg ttttgcttct tcatctacga gtaataacac taaaagtggt tttaatggcg
    17041 gtggtaaagg ttatttaaat caacaggtta tgggtactta ttattctatg tacggtggtg
    17101 gtgctactga tataaggctc gttggtggtg cttgggataa tgagcaaggt ttgctatctc
    17161 gtataattgt cgcaggtggt ggcggtggtt catattctcc ttatactggt ggtgcaggag
    17221 gaggattag
//
LOCUS       DAYAPA010000087        17200 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.087, whole genome shotgun sequence.
ACCESSION   DAYAPA010000087 DAYAPA010000000
VERSION     DAYAPA010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17200
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.087"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(124..648)
                     /locus_tag="ACGI59_002647"
     CDS             complement(124..648)
                     /locus_tag="ACGI59_002647"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFG9740429.1"
                     /translation="MENKNPIVTIEMENGKEIKIELYPNIAPNTVKNFVSLVNEGYYN
                     GIIFHRVIPGFMIQGGCPNGTGMGGPGHSIKGEFSGNGFTNNLKHERGVISMARTMAP
                     NSAGSQFFIMHKNSPHLDGQYAGFGRVIEGMDTVDEIASVRTDSADKPQTPQVMKSVT
                     VETFGIDYSDVEKN"
     gene            complement(713..1465)
                     /locus_tag="ACGI59_002648"
     CDS             complement(713..1465)
                     /locus_tag="ACGI59_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFG9740430.1"
                     /translation="MKLGLCLEGGGAKGAYQAGVVKALYDGGINKFYSISGTSIGAIN
                     GYYLYTGNVDNLEKMWTNIKDIQNGNVKIVNNTVDNSPAIDNLRELDDSNIEEMNFYV
                     NYVEVDNKVVSEKIVDVSKMPRNEAIISISYSGLLPSNPNATLGFKEQFVKDVQEGIY
                     DGFKLDGGLIRSALIEPLIGDNVDKIILISTKYNYELPEDIKKVYDEDKIIVVRPNTQ
                     FAPKDTLNFDDEFCKTIYQEGYEIGKNILDRL"
     gene            complement(1496..2794)
                     /locus_tag="ACGI59_002649"
     CDS             complement(1496..2794)
                     /locus_tag="ACGI59_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HFG9740431.1"
                     /translation="MDSINFAKNLIDFIYDSPSSFHAVESTKEILDKNGFEELVLNQR
                     WNLRVGGKYYVTKNLSAIVAFVVNSEGIERDGFRIIGSHSDSPTFSIKPNAEIEAEKS
                     YLKLNTECYGGAILSTWLDRPLGIAGRVVLKGDSILKPNERLIDFKKPICIIPNLAIH
                     LNRSINDGHSYNKQKDMLPLVGMLNDTLEKDNFLIKQIASNLDVNMDDIIDFDLFLYE
                     FEKGSLIGANDEFISIGRQDNLAMVHASLNALVNTKGQHGVNVMAVFDNEEVGSSTKQ
                     GADSNMLLNILERICISMGKDREGFFESIYSSFMISSDLAHAIHPNIPEKHDPTNKPI
                     MGKGPVIKINASQAYTSDGYSIAIYKNICREANVEYQEFVNRSDERGGSTIGPISSTH
                     IDIPSVDVGSPILAMHSIRELGNVEDHYSIYKTFSKFFEI"
     gene            complement(2811..5075)
                     /locus_tag="ACGI59_002650"
     CDS             complement(2811..5075)
                     /locus_tag="ACGI59_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3553 domain-containing protein"
                     /protein_id="HFG9740432.1"
                     /translation="MNLDTLNPAQREAVEKTEGPVLILAGAGSGKTKVLTTRIAYLIE
                     DKGVQAPNILAITFTNKAANEMRERVEQNIGPETKDMWISTFHSCCVRILRKDINKIG
                     YNRSFVIYDSADQVTLVKDCLKELNLSDKVFEPKAVISAISGAKDKLYTPKQFKDINM
                     ADNRMVKIADIYALYQDRLKRNSALDFDDLILKTVELFKANDEVLAYYRSRFRYIMVD
                     EYQDTSKAQYELIKLLAREHQNICVVGDDDQSIYGWRGADIRNILEFEKDYDNVHVVK
                     LEQNYRSTQVILDAANKVISNNIERKRKKLWSEKKEGELIKIQLTGSEIEEADFIADS
                     IAQIARKENRPYKDFAVLYRANAQARPVEDALNRSQIPYNIYGGTKFYERKEIKDLLA
                     YLRVIQNPQDDISIKRIINVPRRGIGLRTIEKIEDRANLKQESIYSVLIDIETNSDIS
                     TKARASISGFVDIIGTLRTIKEVYPVSKLIEKVLDTTGYMDELVEIRNKNEKDLTGKG
                     EEAQDRIDNLREFISIALEFESSNDDTYENKDLETFLTSIALTSESNDEEDNDRVSLM
                     TIHTSKGLEFPVVFLTGMEEGLFPISRAIKSMSDSQIEEERRLCYVGITRAKEELYMS
                     LTEKRTLYGKTNVAIASRFMEELPEECIERLYKVKKELSYSKASYNMLDKYTKKYMST
                     ISKSKVADKVNATIKDSNKETNPDDIKLGSKVHHPKFGVGTVVSIIGTDVTIAFDQQG
                     IKKINKEYTTLNIL"
     gene            complement(5330..6148)
                     /locus_tag="ACGI59_002651"
     CDS             complement(5330..6148)
                     /locus_tag="ACGI59_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFG9740433.1"
                     /translation="MFKINNLTYQYEKNTNALLNINMDFSKGNVIGIIGSNGSGKSTL
                     FMNLMGILKPTSGEILFKEEKLKYDKRSLYNLRKNVGIVFQDPEKQIFYSKVYDDIAF
                     AMRNIGMDEKTIKERINKALVAVNGIDFIDRPVHFLSYGQKKRVAIASVIAMENEIVL
                     LDEPTAGLDPVSTRSIVDIIKGLNKNNIKIVISSHDMNLMYEICDYIYVLDKGILIDE
                     GKAENVFINENNIIQAGLESPWLVKVHRNMNLPLFKKEEDLYKYWKERELNTNK"
     gene            complement(6141..6839)
                     /gene="cbiQ"
                     /locus_tag="ACGI59_002652"
     CDS             complement(6141..6839)
                     /gene="cbiQ"
                     /locus_tag="ACGI59_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0032778 - P-type cobalt transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HFG9740434.1"
                     /translation="MLIIDKYAYTNRLSKVNPNKKVAIGVIFLIASMVIQNIFILSGI
                     MILMSILVVCVAGIDLKNYLKLLRIPMYFLFLSIGITLVNISFNKADLLYSFEFFSFN
                     VGISKASINMSIHVLFRAMSCLTCVYFCILTTPFNQLIFFFKKLHLPDTFVELSMLIY
                     RFIFIFLEEFSEIYKSQELRFGYINLKTSYKSLGILGSMLYKRLMTRYDDMCISLDIK
                     LYDGKFHIVGDNDV"
     gene            complement(6842..7126)
                     /locus_tag="ACGI59_002653"
     CDS             complement(6842..7126)
                     /locus_tag="ACGI59_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     substrate-binding protein"
                     /protein_id="HFG9740435.1"
                     /translation="MSAKTKTKTNILLLLLVVALIIFPLLVNSGAEYGGADGQAESEI
                     TKINPNYKPWFSSPYEPPSGEIESLLFSAQAALGAGVIGYILGLQKGKRS"
     gene            complement(7116..7868)
                     /locus_tag="ACGI59_002654"
     CDS             complement(7116..7868)
                     /locus_tag="ACGI59_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0000041 - transition metal ion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HFG9740436.1"
                     /translation="MKQNIKLGVIAALMLIILTPVTSNAMHIMEGYLPVKWSIAWGVI
                     FIPFFLFGLKSIGKIVKQDPKKKVLLALCGAFVFVLSALKIPSVTGSCSHPTGVGLGA
                     IMFGPSVMFVLGTIVLIFQALLLAHGGITTLGANAFSMAIIGPIVSFLIFKALKKKDG
                     NNAIPVFLAAAIGDLATYTITSIQLALAFPDPSGGVMASAIKFLGIFFMTQIPIAIAE
                     GILTVIVYNLITENGEKSILENNDKGVKANEC"
     regulatory      complement(8050..8242)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(8374..10146)
                     /locus_tag="ACGI59_002655"
     CDS             complement(8374..10146)
                     /locus_tag="ACGI59_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase activation-like protein"
                     /protein_id="HFG9740437.1"
                     /translation="MKWIYDDYEFENRANNLSWTIAGDYNESIDISEKDYSSKEIGLY
                     FAIIAGARRKYVDWDIVKKYLTNRIRKGYNKDVILGLIQVILNTIVEEKVIKDRPGIE
                     DIRRNAYKDIVSRFTKIHNDNIMDKIKYTFVLYSMDKHPALDSMTRRIVNDIRNIDTN
                     QDIMEILKALDVIYLTYFEYILNGEENSLIDENNVESVEVNFDTFADFMYEELYSDEE
                     ENIIESDIDSISSSMLVEGVGDFDDSKCSNSADRVIYVDEDTANKIYSKIEHYYGKTY
                     LSKGEIKKIETQNCRNVHEGCRIHFTDGVLRSECNNVFQLKYVTRQKENNLGKFREHA
                     RMYRQQIKRLKESLSRILIEENAKSRVYSDCGTILANRAWRVGRSNNNKIFYKDIENE
                     KGKYVIDILLDASGSQSRNQGNVAIQAYIIANALTEVGIPNRVMGFSSFLDYTILKRF
                     KDYDDSIKNSENIFEYFCAGNNRDGLAIKSICSGLINRDEENKILIVLSDGRPNDVKI
                     GKSSERTLRGEKAYRGIVGLRDTANEVRKARKQNILVLGVFTGKESELEAERLIYGND
                     FIYSRDITRFSDVVSMYLKKIIRN"
     gene            complement(10160..11089)
                     /locus_tag="ACGI59_002656"
     CDS             complement(10160..11089)
                     /locus_tag="ACGI59_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFG9740438.1"
                     /translation="MDIIDNLRVQGVDEKLIEDVLYFRNYYGLEKDLEYRVTKSKTYF
                     YGKDILSMCIAAILEEENILLSGPKATGKNLLADNLGEIFNRPQWNTSFHINTDSSTL
                     IGTDTFIDNEVKLRRGSVYECAINGGFGVFDEINMAKNDAIVVVHSALDYRRIIDVPG
                     YERVNLHPATRFIGTMNYEYAGTKELNEALVSRFMVIDIPPIEEDKLMMILKNEFSDA
                     DEEKLIHFAGIFLDLQLKSQNGEISSKAIDLRGLMASLKTIRRGLKPTLAINMGLTGK
                     TFDVYEKEMVGDVIKTRIPNKWESIDVFPISHI"
     gene            11310..11495
                     /locus_tag="ACGI59_002657"
     CDS             11310..11495
                     /locus_tag="ACGI59_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFG9740439.1"
                     /translation="MKYGCPEVAKKWGISERRVQKLCEESRIPGVSKLGYMWLIPKNA
                     EKPVDGRTKRRKEVQYE"
     gene            11488..12189
                     /locus_tag="ACGI59_002658"
     CDS             11488..12189
                     /locus_tag="ACGI59_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFG9740440.1"
                     /translation="MNSVLIIDDDKELCALMKKCVEQENLSAITAHCGAEGLHLVDKN
                     RHTLSLVILDIMMPDIDGFQVLKKIRETSNIPVIMLTAKNDEDDKVSGLRLGADDYLT
                     KPFSINELMARVNSLIRRYTMLNPAFITDTDCIIINGMIIDKTNRIVSVNNIPVQLTS
                     KEFDLLAFLASNKGRVFTKKQIYTQVWEEEYAFDDSNIMSFISKLRKKIEPDPNHPFY
                     ILTVRGVGYRFNKEA"
     gene            12191..13114
                     /locus_tag="ACGI59_002659"
     CDS             12191..13114
                     /locus_tag="ACGI59_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFG9740441.1"
                     /translation="MNTSVYLLLILVLSLIIIAYLVSVLLRVRIQLTLIKDALEDIKN
                     GNLNRRILTDENDITKQICYDINEIAINSQFQLIQQKQSEQAYKQLMTSLSHDVKTPL
                     ASLVGYLEAVESKIVVGEEKDEYIHVASNKAHYLKNFVENLFEWVKLDSKEQVFHFDI
                     FDLNELSRNIISDWIPVLESSHFEYEFDIPEIEYFLRIDANAYTRIINNLLQNIITHS
                     SGNKMTLRIFENKEQAQIIITDNGKGISSDNLPHIFERLYQCDHSRASKGNGLGLAIA
                     KELINAHKGNITANSTPGMGTEFTILLPKAL"
     gene            13271..14194
                     /locus_tag="ACGI59_002660"
     CDS             13271..14194
                     /locus_tag="ACGI59_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019639192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9740442.1"
                     /translation="MNDFVIETKQLTKIYGNQTVVDKVNLHAKKGRIYGLLGRNGAGK
                     TTIMKMILGLTSITYGEVDVFGENIKGREKQVYPRIGAIIETPGFYPNLTGTENLEIF
                     AKLRGTAAPNSVKRALEIVGLPYKNKKLFSEYSLGMKQRLGIANAILHDPELLVLDEP
                     TNGLDPIGIAEMRKFIRDLSVEHGKTILISSHILSEISLLADDIGIIDHGVLLEESSM
                     SELEKKNSKYILLQVSDIPKTSLILERQFNTKDYSVQDDYTLHLYNTNLDMGEINKAL
                     VMQNVTVISSQLCNDTLEDYFKKITGGEGIA"
     gene            14187..14924
                     /locus_tag="ACGI59_002661"
     CDS             14187..14924
                     /locus_tag="ACGI59_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9740443.1"
                     /translation="MLKLIQTEFLKLRRRKLIWLMLLSALIMPFFALLYFNYFGKTGV
                     EPIKFYKWSAFGYTLWIILPVVLGIFCTMLIHEENQYDMLKQLWIVPISKMGYFFSKF
                     FVVLIYSICFMLITAVASVLFSVLSGYVVFSWASILYLLKKCLEIGVITAFVMLPILA
                     IATSQKGYILPICITLVYAFSGFVIMTINMYLHPLSSMGVIIMRNKDIPGLIFSQEIN
                     VPLAFLCICIWSIVSVLFANTALGRRK"
     gene            14935..15708
                     /locus_tag="ACGI59_002662"
     CDS             14935..15708
                     /locus_tag="ACGI59_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9740444.1"
                     /translation="MWKLLWAECQKIRRSKIVWITVFASIMIAVIVFAGGQDVYNDPD
                     LHYGLKSVYDGSRYIDNAGWYMDAVQPWSTFFVLPAVIALLGSYIICREEEEDTIKSL
                     RLIPINETKLTIAKMIITFIFSILLYLLLFTITFLTESVLHFSDLSTKLVLSCMKEYF
                     LDGIGVFFAISPIIALVSRMKKGYWLALVFTEVYSIAGLFAGMSNVLQTFFPIAAIFN
                     ISGYYITTGEKIMGSIISLLLCACLSAFILKGLKHNEKN"
     gene            complement(16249..16857)
                     /locus_tag="ACGI59_002663"
     CDS             complement(16249..16857)
                     /locus_tag="ACGI59_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFG9740445.1"
                     /translation="MSVINSFILQYGLISVFVLIMIEYACFPLPSEVVLPLCGAIAAR
                     NHFGFLTILILSIIAGILGSIFCYTVGNWGGKSIINKIIEICPKAKKGIFASQDYFNK
                     YSSISVCVCRLIPLCRTYISFIAGIAGQNIITFVISSIVGITIWNTSLISIGYIFSES
                     WVRIMSHYNDYKFLVLIIPISIIFIGFVIKSFIHKKHHKIRL"
     gene            complement(17035..17151)
                     /gene="rrf"
                     /locus_tag="ACGI59_002664"
     rRNA            complement(17035..17151)
                     /gene="rrf"
                     /locus_tag="ACGI59_002664"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         5533 a         2987 c         2184 g         6496 t
ORIGIN      
        1 aaataaatta aaaagtgaaa tgtatttgtc taaaaacata ataacatttc actttatcct
       61 catttttaag taatatatat ttttaatttt tatttataac taaatctagt ttttatataa
      121 attttagttt ttttctacat ctgagtaatc tattccaaaa gtttctacag taacagattt
      181 cataacttgt ggagtttgtg gcttatcagc tgaatctgtt ctaactgatg ctatttcatc
      241 aactgtatcc ataccttcta tcactctacc aaaacctgca tactgaccat ctaagtgtgg
      301 tgagttttta tgcattatga aaaattgaga accagctgaa ttaggtgcca tagttctagc
      361 cattgatatg actcctcttt catgttttaa attgttagta aatccattac cactaaattc
      421 accttttatt gagtgacctg gaccacccat tcctgtacca tttgggcatc ctccttgtat
      481 cataaatcct ggtattactc tatgaaatat tattccattg taatatcctt catttactaa
      541 tgatacaaag tttttaactg tatttggagc tatatttgga tataactcaa ttttgatttc
      601 ttttccattt tccatttcta tagttactat aggattttta ttttccataa tttattacct
      661 tccttttcat tagttaaatt acataattac atttattata ttatactcta aattaaagtc
      721 tatcaagtat attttttcct atttcatatc cttcttgata aatagtttta caaaactcat
      781 catcaaagtt aagcgtgtct ttaggagcaa attgagtatt aggtctaact actattattt
      841 tatcttcatc atatactttt ttaatatctt ctggcaactc ataattatat tttgtggata
      901 ttaaaataat tttatccaca ttatccccaa ttaatggttc aattaatgca ctcctaatta
      961 aacctccatc taatttgaaa ccatcataaa ttccttcttg cacatctttc acaaattgtt
     1021 ctttaaatcc aagagttgcg tttggatttg atggtaacaa cccactgtat gaaatgctga
     1081 taatggcttc attacgaggc atttttgata cgtcaacaat cttttctgat actactttat
     1141 tatcaacttc aacataatta acataaaagt tcatttcttc tatattacta tcatctagtt
     1201 cccttaggtt gtctatagca ggagaattat ccacagtatt atttactatt tttacatttc
     1261 cattctggat atcttttata tttgtccaca ttttctccaa gttatcaaca ttccctgtgt
     1321 acaaataata tccatttatt gctcctattg atgtccctga aatcgaataa aatttattta
     1381 ttcctccatc atacaaggct ttaactactc ctgcttgata agcccctttt gctcctccac
     1441 cttctaaaca taatcctaat ttcatagtct catctccaaa ttgtttatct tatatttata
     1501 tttcaaaaaa cttactaaat gttttatata tactatagtg gtcctcaaca tttcctaatt
     1561 ctcttataga atgcattgct aaaattggac ttcccacatc cacagatggt atatctatat
     1621 gagtagaaga tattggacct atagtacttc ctcctctttc atctgaccta ttaacaaatt
     1681 cttgatactc aacattagct tctctacata tatttttata aatagctata gaatatccat
     1741 cactagtata tgcctgacta gcatttatct ttataaccgg accttttccc attattggtt
     1801 tattagttgg gtcatgcttt tctggaatgt ttggatgaat agcatgagct aaatctgatg
     1861 atatcataaa agatgaataa atagactcaa agaatccctc tctatcctta cccattgata
     1921 tgcaaattct ctccaatata tttaacaaca tgtttgaatc tgcaccttgt ttagtagagc
     1981 taccaacttc ttcattgtca aatacagcca ttacatttac accatgttga cctttagtat
     2041 taacaagagc attcaaacta gcatgtacca ttgcaagatt atcttgtctc ccaatagata
     2101 taaattcatc attagcccct attaaacttc ctttttcaaa ttcatataaa aataaatcaa
     2161 aatctataat atcgtccata tttacatcta agttagatgc tatttgcttt ataagaaaat
     2221 tatctttttc taatgtatca tttaacatcc caactaaagg taacatatct ttttgcttat
     2281 tatatgagtg accatcattt atacttctat ttaaatgtat tgctagattt ggtattatac
     2341 aaattggctt tttaaagtct ataagccttt catttggttt taatatacta tcacctttta
     2401 aaactactct accagctatc ccaagaggtc tatccaacca tgtacttaat attgctcctc
     2461 catagcattc tgtatttaac ttcaaataag atttttcagc ttctatttct gcatttggtt
     2521 taatactaaa tgtaggagaa tctgaatgag agcctattat tctaaatcca tctctttcta
     2581 taccttctga atttactaca aaagctacta tagctgataa attcttagta acataatact
     2641 ttccaccaac tcttagattc catctttgat tcaaaactaa ttcttcaaat ccatttttat
     2701 ctaagatttc cttagtactc tcgactgcat gaaaagacga cggactatca tatatgaagt
     2761 ctattaagtt tttagcaaag tttatactat ccattcctca tttacctcca ttataaaata
     2821 tttagtgttg tatattcttt atttattttt ttaattccct gttggtcaaa agctatagta
     2881 acatctgtac caattataga aaccactgtc ccaaccccaa atttaggatg atgtacctta
     2941 gaaccaagtt ttatatcatc tggattagtt tctttattag aatcttttat tgtagcatta
     3001 actttatctg caactttaga tttgcttata gtactcatat actttttagt atatttatct
     3061 aacatattat aacttgcttt agaatagctt agttcttttt ttactttgta caatctttct
     3121 atacattctt ctggcagttc ttccataaat cttgaagcta ttgcaacatt tgttttacca
     3181 tataatgttc tcttctctgt caatgacata tatagctctt ctttagctct agttatacca
     3241 acataacaaa gtcttctctc ttcttctatt tgtgaatcac tcatagattt tatagctctt
     3301 gatataggaa ataacccttc ttccatacct gttaaaaaca caacagggaa ctctaaacct
     3361 ttggatgtat gaattgtcat aagcgaaacc ctatcattgt cttcttcatc atttgattca
     3421 gatgtaagtg ctatacttgt taggaatgtc tctaagtctt tattttcata agtatcatca
     3481 ttactacttt caaactccaa agctatagaa ataaattctc tcaaattatc aattctgtct
     3541 tgagcttctt cacctttgcc tgttaaatct ttttcatttt tatttcttat ctctacaagc
     3601 tcgtccatat atcctgtagt atccaagact ttttctataa gtttgctaac aggataaact
     3661 tcttttatag ttctaagagt ccctataata tcaacaaatc cacttatact tgcccttgcc
     3721 ttagtagata tatctgaatt tgtttctata tcaattaata ctgagtatat actttcctgt
     3781 tttaagttag ctctatcttc aattttttct atagttctaa gccctatacc tcttctagga
     3841 acatttataa tcctttttat agatatatca tcttgtggat tttgaataac tcttaaatac
     3901 gccaacaaat cctttatttc ttttctctca tagaattttg taccaccata tatattgtat
     3961 ggaatttgac ttctatttaa agcgtcctct acaggtcttg cctgtgcatt tgctctataa
     4021 agtactgcaa aatccttata aggtctattt tcttttctag ctatttgtgc aattgaatca
     4081 gcaatgaaat cagcctcttc tatttcacta ccagttaatt gtatctttat aagttctcct
     4141 tctttttttt cactccaaag cttttttctt tttctttcta tattattaga tataacttta
     4201 ttagctgcat ctaatataac ttgtgtagac ctataatttt gttccaactt tacaacatgt
     4261 acattgtcat aatctttctc aaattcaagt atgtttctta tatctgcacc tctccatcca
     4321 taaatacttt ggtcatcatc tccaacaaca caaatatttt gatgttctct tgctaataat
     4381 tttatcaatt catattgagc tttacttgta tcttggtact catctaccat tatgtatcta
     4441 aatctacttc tgtaatatgc taacacttca tcattagcct tgaaaagttc tacagtttta
     4501 agtattaaat catcaaagtc aagtgcacta tttcttttta aacggtcttg atatagcgca
     4561 taaatatctg caatcttaac cattctatta tctgccatat tgatatcttt aaactgcttt
     4621 ggagtataaa gtttgtcttt tgctcctgaa attgcactta taactgcttt tggttcaaac
     4681 actttatcac ttaaatttaa ttcttttaga cagtctttaa ccaatgtaac ttggtctgct
     4741 gaatcataaa ttacaaaact tctattgtaa ccaatcttat ttatatcttt tcttagtata
     4801 cgaacacagc aagaatggaa tgtacttatc cacatatcct tagtttcagg acctatattt
     4861 tgttcaactc tctctctcat ttcatttgca gctttgtttg taaatgttat agctaatata
     4921 tttggtgctt gtacaccttt atcttctata agatatgcta ttcttgttgt taatacctta
     4981 gttttacctg aaccagcacc agccagtata agtactggac cttcagtctt ttctactgct
     5041 tctctttgag caggatttaa tgtatctaaa ttcatatttc tcctcctgtt aacagccata
     5101 aatatatttt aatctaaaat atgaattact tcaaattata attatgtatt atattaatag
     5161 attatttcca atatattaca attaaataac atttttccat ataatttatt tcattgttta
     5221 ataaaaaaaa taaccttatg atttggggaa tcataaggca cctttaaggg gtctaggtta
     5281 agggttatta tatataaatc tattttttta aaataggttt agggcttaat tatttatttg
     5341 tattaagttc tctttccttc caatacttat acaaatcttc ttctttctta aataagggta
     5401 aattcatatt tctatgtact tttactaacc atggggattc taggccagct tgtataatat
     5461 tattttcatt tataaacaca ttttctgctt ttccttcatc aatcaaaatc cccttatcca
     5521 atacatatat gtaatcacat atttcataca ttaaattcat atcatgactg gatattacta
     5581 tttttatatt atttttattt aatcccttta ttatatctac aatagaccta gtacttactg
     5641 ggtctaaacc agctgtaggt tcatcaagta atacaatttc attttccatt gctataactg
     5701 atgctattgc cactcttttt ttctgtccat agctaagaaa atgtactggc ctgtctataa
     5761 aatctattcc attaacagct actagagctt tgtttattct ctctttaatt gttttttcat
     5821 ccataccaat gtttctcatt gcaaaggcta tatcatcata tacttttgaa taaaaaattt
     5881 gtttttctgg gtcttggaag acaataccta cattttttct aaggttgtat aaacttctct
     5941 tatcatattt taacttttct tctttaaaaa gaatttctcc agatgtaggc tttaatatac
     6001 ccattaaatt cataaataat gttgattttc ctgaaccatt tgaacctatt atacctataa
     6061 catttccctt tgaaaaatcc atattaatgt ttaatagtgc attggtattt ttttcatatt
     6121 gatacgttaa attatttatt ttaaacatca ttatccccta ctatgtgaaa tttaccatca
     6181 tacaatttta tgtctaatga tatacacata tcatcatacc ttgtcataag tcttttgtac
     6241 agcatacttc caagaatacc taaggattta tatgaagttt ttaaatttat atatccaaat
     6301 cttagctctt gtgacttgta tatttctgaa aattcttcta aaaatatgaa tataaaccta
     6361 tatattaaca ttgataactc gacaaaagtg tcaggtaaat gaagtttttt aaaaaagaat
     6421 attaattgat taaaaggtgt tgtaagtata caaaaataca cacaagtcaa acaagacatc
     6481 gctctaaata acacatgaat agacatgtta atagacgcct ttgatattcc tacattaaaa
     6541 ctaaaaaatt caaaactata taataaatct gctttattaa aagatatatt tacaagagtt
     6601 attcctatac taagaaataa aaagtacatt ggtattctaa gtaattttaa ataatttttt
     6661 aaatctattc cagccacaca aacaaccaaa atactcatta aaatcataat tccacttaaa
     6721 ataaatatat tttgtattac catagaagca atcagaaaaa ttactcctat ggctactttt
     6781 ttgtttggat ttactttact caatctattt gtatatgcat atttatcaat tataagcatg
     6841 attaacttct ctttcctttt tgaagaccta atatgtatcc tattacacca gcccctaaag
     6901 ctgcttgtgc agagaataat aaactttcta tttctccact tggtggctca tatggactgc
     6961 taaaccatgg tttgtaattt ggatttatct tagtaatttc actttcagct tgaccatctg
     7021 ctcctccata ttcagcacct gagttgacaa gtaatgggaa tataataaga gctactacta
     7081 ataaaagtaa taatatgtta gtttttgttt ttgttttagc actcattagc tttaactcct
     7141 ttatcattat tttctagtat gcttttttct ccgttttctg taattaaatt atatacaatt
     7201 acagtaagta taccttctgc tattgctatt ggtatttgag tcataaagaa aattcctaaa
     7261 aatttaattg ctgaagccat taccccacct gatgggtctg ggaaagccag tgctaattgt
     7321 attgaagtta ttgtatatgt ggctaagtct cctattgctg ctgcaagaaa tactggaata
     7381 gcattatttc catctttttt ctttaaagcc ttaaatatta ggaatgatac gattggccct
     7441 attatcgcca ttgaaaaagc attagctcct aaagtagtta ttcctccatg tgctaataac
     7501 aatgcttgga atattaaaac tatagttcca agaacaaaca ttacgcttgg tccaaacatt
     7561 attgctccaa gacctacacc agtaggatgt gaacaacttc ctgttactga aggtattttt
     7621 agtgctgata atacaaacac aaatgctcca cataaagcta ataatacttt ctttttaggg
     7681 tcttgcttta ctattttacc tattgatttt aatccgaata agaaaaatgg tataaatata
     7741 actccccatg ctatagacca ttttactggt aagtaacctt ccattatatg cattgcattt
     7801 gatgtaactg gagttaaaat gattaacata agtgcagcta taacccctaa tttaatattc
     7861 tgtttcatct tagtatccct ctctcccttt ctaataatta aaacataata atacatgtct
     7921 gatttattta ataaaataaa aaagcctaac tcgatggtta ggcttctaat tttccacaat
     7981 ctaaatacaa acacataaaa caaacatatc tgtataatta ttataagaaa ctttctccat
     8041 ccccgaagaa attactttaa tattttaggc aggtctcctg acttagcttc atcctactcc
     8101 ttttccttcc catgagatat aaaaaatatc atcatagtgg ctaacaaaag tttcgtctgc
     8161 atcacagtag cgggggctgt aaaggaattt aacctttttc cctattaatc ttgcttaaag
     8221 aacctataat attatatatt taatttttaa attattctta aatcattttc tatataaatc
     8281 attgtatacc ttttctgtga aaatttctac attttttata aattttattc atttttatat
     8341 ttttatgtat ataaattctt ttttgtaagt ttattaattt ctaattatct tctttaaata
     8401 cattgaaact acatcagaaa atctagttat atctcttgaa tatataaaat catttccata
     8461 tattaatctt tcagcttcaa gttctgattc ttttcctgta aatactccta atactaatat
     8521 attttgcttt ctagcttttc tcacttcatt ggctgtatct ctcaaaccaa ctatacccct
     8581 ataagccttt tctcctctta atgttctctc actacttttt cctatcttta catcatttgg
     8641 tcttccatcg ctgagaacta tcaggatttt attttcttca tctctattta ttagaccact
     8701 acatatagat tttattgcca aaccatctct attattacca gcacaaaaat attcaaatat
     8761 attttcacta ttttttatac tatcatcata atctttaaat ctttttaata ttgtatagtc
     8821 taaaaaacta gaaaatccca tcactctatt tggtatgcca acttctgtta gagcatttgc
     8881 aattatgtaa gcttgaattg ccacatttcc ctgatttcta ctttgagagc cacttgcatc
     8941 caataatata tcaataacgt atttcccttt ttcattttct atatctttat aaaatatttt
     9001 attattatta cttctaccaa ctctccaagc tctattggct aatatagtac cacaatcaga
     9061 atatactcta cttttagcat tttcttctat taaaattctt gacagacttt cttttaatct
     9121 ctttatttgc tgtctataca ttctagcatg ttctctaaat tttcctaaat tattttcttt
     9181 ttgtcttgtt acatatttta gttgaaatac attattacac tcacttctaa gaactccatc
     9241 tgtaaagtgt attctacaac cttcatgaac atttctacaa ttttgtgtct caattttttt
     9301 aatttcacct ttactcaaat aagtttttcc ataataatgt tctatttttg aatatatctt
     9361 atttgcagtg tcttcatcaa catatataac tcggtctgct gaattgctac atttagaatc
     9421 atcaaaatcc cctacacctt ctacaagcat ggatgaagat attgaatcta tatcactttc
     9481 aataatattt tcttcttcat cactataaag ttcttcatac atgaaatcag caaatgtatc
     9541 aaaatttact tctacacttt ctacattatt ttcatcaata agactatttt cttctccatt
     9601 taatatgtat tcaaaataag ttaaatatat aacatctaat gcttttaaaa tctccataat
     9661 atcttgattg gtatctatat ttcttatatc attaactatt cttctagtca tactatctaa
     9721 tgctgggtgc ttgtccatac tataaagtac aaatgtgtat ttaattttat ccataatatt
     9781 atcattgtgt attttggtaa aacgtgaaac aatatcttta taagcattcc ttcttatgtc
     9841 ttctatccct ggcctgtctt taatgacctt ttcttcaact attgtattta atataacttg
     9901 aattagacct agtataacat ctttgttgta tccttttctt attctatttg taagatattt
     9961 tttaactata tcccaatcta catactttct tctagcacct gcaattattg cgaaatatag
    10021 acctatttct tttgaagagt aatctttttc tgatatatct atactttcat tgtaatcacc
    10081 agctattgtc catgataggt tattagctct attttcaaat tcataatcat catatatcca
    10141 tttcataatc tcacctatat taaatatgac ttattggaaa cacatctata ctctcccatt
    10201 tattaggtat tctagtttta attacatctc caaccatttc tttctcatat acatcaaaag
    10261 tctttcctgt aagtcccata ttaattgcaa gagttggttt taatcctctt cttatagtct
    10321 taagagatgc cataagacca cgtaaatcaa tagctttact agatatctct ccattttgag
    10381 acttcaattg taaatctaaa aatattcctg caaagtgtat taatttttct tcatcagcat
    10441 ctgaaaattc attttttaaa atcatcatta acttatcttc ttctatagga ggaatatcta
    10501 ttaccataaa tctagatacc agtgcttcat taagttcttt tgttcctgca tattcataat
    10561 tcatagtccc aataaatctt gttgctggat gtagattgac tctctcatac ccaggtacat
    10621 caattattct tctatagtct aaagctgaat gtaccacaac tatagcatca ttttttgcca
    10681 tattaatttc atcaaacacc ccaaaacctc cattaatagc acactcataa actgagcctc
    10741 ttctaagttt aacttcattg tctataaatg tatctgtacc tataagtgtt gaactgtctg
    10801 tatttatatg aaatgatgta ttccactgag gtctattaaa tatttcccct agattatcag
    10861 caagtaagtt ttttcctgtt gcctttggtc ccgataataa tatattttct tcttctaaaa
    10921 tagctgctat acacatagac aagatatctt taccataaaa ataagtttta gactttgtta
    10981 ctctgtattc taaatctttt tctaaaccat aataatttct aaaatacagt acatcttcaa
    11041 taagtttttc atctacccct tgcaccctta gattatctat tatatccata tttttacccc
    11101 ttttatataa atagtatttg tttcatttct tatcaccatt ttagcataaa aggatataag
    11161 ctaaaattga ttttttaatc tccccatttt aagtaaaacc atgcaccatt ttatcactac
    11221 aaaattaaga gtactactta ctgatatttc ttgattttgt tcgtctatac gtatataata
    11281 tatgctgata aattatgagg aaaggatgat tgaaatatgg atgcccagaa gtagcaaaaa
    11341 aatggggtat ttctgaaaga agagtacaaa aactatgtga agaaagtcgc ataccaggag
    11401 tttcaaaact cgggtatatg tggctgatac caaagaacgc agaaaagcct gttgatggta
    11461 gaacaaaaag aagaaaggaa gtacaatatg aatagcgttt tgattataga tgatgacaaa
    11521 gaactttgtg ctcttatgaa aaaatgtgtt gagcaagaaa atttatctgc tattactgca
    11581 cattgtggtg cagaaggtct acatttggtc gataaaaata gacacacact ctctcttgta
    11641 attttggaca taatgatgcc agatatagac ggttttcaag ttctgaaaaa aattagagaa
    11701 acaagtaata ttccagtaat tatgcttact gctaaaaatg acgaggatga taaagtttcc
    11761 ggactacgtt taggtgctga cgattaccta acaaaaccgt tcagcataaa cgaacttatg
    11821 gctcgtgtca attctcttat tcgtcggtat actatgttaa accctgcttt tataacagac
    11881 acagattgca taataataaa tggcatgatt attgacaaga caaaccgaat tgtttctgtc
    11941 aataatattc ctgttcaact tacaagcaaa gaatttgatt tacttgcttt tctggcttct
    12001 aataagggac gggtgtttac caaaaaacag atttatacac aagtatggga agaagaatat
    12061 gcttttgacg atagtaacat catgtctttc atcagtaaat tgagaaaaaa gattgaacca
    12121 gaccccaacc atccatttta tattttgaca gttcgtggtg tcggttatcg ttttaataag
    12181 gaggcttaac atgaatacaa gtgtctatct gcttttgata ttggttctta gcctgattat
    12241 tatagcgtac cttgtttctg tcctgctacg tgtccgaata caacttacac ttataaaaga
    12301 tgctttagag gatataaaaa atggaaacct taatcgacgt attttaacag atgaaaacga
    12361 cattacaaaa caaatttgct atgatattaa cgaaatagca atcaatagcc aatttcaact
    12421 tattcagcaa aaacaatcag aacaagccta caaacagcta atgacaagtc tctcccatga
    12481 tgtaaaaact ccacttgctt ctttggtcgg ttatttggaa gcagtagaaa gtaaaattgt
    12541 tgtaggagag gaaaaagacg agtatatcca tgttgcgtct aataaggctc attacttaaa
    12601 gaattttgta gaaaatctat ttgaatgggt aaaattggat tctaaggaac aagtttttca
    12661 ttttgatatt tttgacttga atgaattatc ccgtaatatt atatctgatt ggatacctgt
    12721 tttagaaagc agtcattttg aatatgaatt tgatatacca gaaatagagt acttcttacg
    12781 tatagacgca aatgcatata cccgtatcat taataaccta ttacaaaata ttattaccca
    12841 tagtagtggc aacaaaatga cattgagaat ttttgaaaac aaagagcaag ctcaaattat
    12901 aataacagat aatgggaaag gtatatcttc agacaatctc ccacatattt ttgaaaggtt
    12961 gtaccaatgc gaccattcac gagcttcaaa gggaaatggt ttaggcttgg cgattgcaaa
    13021 ggaacttatt aacgctcata agggaaatat taccgccaac agcactcccg gtatgggaac
    13081 agaatttaca atattgcttc ctaaagccct gtaaaagtac gaggctttct tactctaaaa
    13141 caagataaaa gcaagattat ggcaaggttt tggcaaggtt catgttttat aattctaatt
    13201 gtagttgatt ttgacgttgt atcaactatc ttttgtagaa acaaatttat taaaagtgag
    13261 gaaattatat atgaatgatt ttgtgattga aacaaagcaa cttacaaaaa tttatgggaa
    13321 tcaaacagta gttgacaaag tcaatctcca tgcaaaaaaa ggccgaatat atggtctgtt
    13381 aggacgcaac ggagctggga aaaccacaat tatgaaaatg attttaggat tgacttcaat
    13441 cacgtatggc gaggtagatg tatttggaga gaatattaag ggacgtgaaa aacaagtata
    13501 tccccgtatc ggtgcaatta tcgaaacgcc aggattttat cctaatctga caggaacgga
    13561 gaacttggag atttttgcaa aactgcgtgg tacagccgcc cctaattctg taaaaagagc
    13621 attggaaatt gtaggcttac cttataaaaa taaaaagctg tttagtgaat attcacttgg
    13681 catgaaacag cgacttggta ttgccaacgc tatcctgcat gacccagaac tgttagtttt
    13741 agatgagcca accaatggtc ttgaccctat tggtattgcg gagatgagaa agtttattag
    13801 ggacttgagc gttgagcatg gaaagacaat tcttatttcc agtcatattc tttcagaaat
    13861 ctcattgttg gctgatgata ttggaattat cgaccacggt gttcttttgg aagaaagtag
    13921 tatgagtgaa ctagaaaaga aaaacagtaa atatatcctg ctacaagttt cagatattcc
    13981 gaagacatca cttattttag agcgtcaatt taacacgaag gattattccg ttcaagatga
    14041 ctacacgtta cacctttata acactaatct ggatatgggt gaaatcaata aagcacttgt
    14101 aatgcagaat gtaactgtta ttagttctca actttgtaat gatactttag aggactactt
    14161 taaaaaaatc acaggaggag agggcattgc ttaaacttat tcaaacagaa tttcttaaac
    14221 tgcgtcgtag gaaacttatt tggctgatgt tgttgtctgc tctcattatg ccgtttttcg
    14281 cactactata cttcaattat tttggtaaaa caggtgttga gccaataaag ttttataaat
    14341 ggtctgcatt tggttataca ctttggatta ttttgcctgt tgttttgggt atattttgca
    14401 ccatgcttat acacgaagaa aaccagtatg atatgctcaa acagctttgg attgtaccta
    14461 ttagtaaaat gggatacttt tttagtaaat ttttcgttgt tttgatttac tctatttgtt
    14521 ttatgctgat tacagccgtt gcttctgtgt tatttagtgt actttctgga tatgtcgtat
    14581 ttagctgggc aagcatttta tatttgttga aaaaatgttt agaaattggt gtaatcactg
    14641 cttttgtaat gttgccgatt ttggcaatcg ctacatctca aaaaggatac attctcccaa
    14701 tttgtatcac attggtatat gccttttccg gttttgttat aatgacaata aatatgtatt
    14761 tacacccatt atccagtatg ggagttatta tcatgagaaa taaagacatt ccaggtctta
    14821 tattttcaca ggaaattaat gtcccattag catttctttg tatttgtatt tggagtattg
    14881 tatcagtatt gtttgcaaac actgcactag gaagaagaaa gtgaggtgta gataatgtgg
    14941 aaattacttt gggctgaatg tcaaaaaatt cgtcgttcaa aaattgtttg gattacagtt
    15001 tttgcatcca ttatgatagc agtcatagtt tttgcaggag ggcaggatgt atataatgac
    15061 ccagatttac attatggctt aaagagcgtt tatgacggtt cacggtatat agacaatgca
    15121 ggttggtata tggacgcagt acaaccttgg tcaacttttt ttgttcttcc tgctgtcatt
    15181 gccctattag gaagctatat tatttgtcgt gaagaagagg aggacactat taaatccctg
    15241 cgtcttattc ccataaatga aacaaaatta accatagcaa aaatgattat tacttttata
    15301 ttcagtatac ttctttattt gttactcttt actataacat ttttgactga atccgtttta
    15361 catttttccg atttatcgac aaaactggtt ttaagttgca tgaaagagta ttttcttgat
    15421 ggcataggtg tattctttgc aatttctccc attattgcct tggtatcgcg catgaaaaaa
    15481 ggatattggc ttgcattagt attcacagaa gtttattcta ttgctggact atttgcgggt
    15541 atgtcaaatg tcttacaaac cttttttcct attgcggcaa tatttaatat ttctggatac
    15601 tatataacta ctggagaaaa aattatgggg agtatcataa gtttattact ttgtgcctgc
    15661 ctttctgcat ttatacttaa agggctaaaa cacaatgaaa aaaattgaaa taggtatatc
    15721 aaaatgaaaa agataaaaaa actgattatt ctaattgtat tcttgtttct ttttggcatg
    15781 ttacactgtt atactgaatg gaaattcaac aataacccac ctaaaggggt aatagaaaga
    15841 ataaatgaat ggactgataa gattggactg gattagtaac ttatgttaat tatagtgaat
    15901 gtaatattag caagttattg tttcgtattt taatactata actttaaata tttggtttta
    15961 atcttccaaa cgaatataaa aaaactgtca cagaggcaac atatttttga atatttataa
    16021 agtggtagtt ttgattttca aagctgccgc cttttcttct atataagcaa acttatcctc
    16081 aacaaagtgt aaaacatggt acattttaag aaaaaagtac cacagacaca agcaataaaa
    16141 gaaacgccga gaacctacaa aaacgtagtg cttatgcaga tttacgacgc cttctatttt
    16201 ctcaaccgtc ttaatgacct tgtacaaaag aaatttttct aatatttttt ataatcttat
    16261 cttgtgatgt tttttatgta taaatgactt aataacaaat ccaataaaaa ttatggatat
    16321 tggtataatt agaacgagga atttataatc attataatgt gacattattc ttacccaact
    16381 ctcagaaaaa atatatccta tactgattaa tgaagtattc catatggtta ttcccacgat
    16441 agatgatatt acaaaagtta taatattttg acctgctata cctgctataa aagatatgta
    16501 tgttctacat aaagggatta atctacatac acaaacagaa atagaagagt atttgttaaa
    16561 ataatcttga gatgcaaata ttcctttctt tgcctttgga catatctcta ttattttatt
    16621 tatgatagat ttacctcccc aatttcccac tgtataacaa aatatcgaac caagtatacc
    16681 tgcaattata cttaaaatta aaattgtcag aaatccaaaa tgatttcttg cagcaattgc
    16741 tccacataaa ggtaaaacaa cttcgctagg taatggaaaa caagcatact caatcataat
    16801 taatacaaat acagagatta atccatattg taaaataaaa ctattgatta cagacactta
    16861 cttatctatc tcctttctta tttaaaggtt ttaagggtac atgttctaag gattctcacc
    16921 ctatatttaa agaatcaatc tctttaaact attatatgta tttacacaaa gcatttataa
    16981 catactttaa aaatagtacc ttaaaataag aaacttaata aataaaaaaa gattacgtgg
    17041 ctacgtccta ctctcccagg cagtttccca ccaagtacca tcagcgctag agagcttaac
    17101 ttctgtgttc ggaatgggaa caggtgtatc ctctctgcta ttgtaaccac ataatcttta
    17161 tgcttaatat ttctattaag tttttaataa gtttatagaa
//
LOCUS       DAYAPA010000088        16757 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.088, whole genome shotgun sequence.
ACCESSION   DAYAPA010000088 DAYAPA010000000
VERSION     DAYAPA010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16757
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.088"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            249..707
                     /locus_tag="ACGI59_002665"
     CDS             249..707
                     /locus_tag="ACGI59_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFG9740446.1"
                     /translation="MEFINFVSDKVIDLKMDCKTKEDFFKEIHNKVFDLGFVKEEFGE
                     KILARENVFPTGLNLGDYGVAIPHTDAEYIKEQFISVCTFNEPVVFSSMEDQDEKVPV
                     NLAFVLGLNQPHSQLSVLTELMGIMQNKELVDKLMSSTDKEEVLKTLKSL"
     gene            768..1043
                     /locus_tag="ACGI59_002666"
     CDS             768..1043
                     /locus_tag="ACGI59_002666"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFG9740447.1"
                     /translation="MKKILVACGAGIATSTVVCDKVERLVKENNIQAEVIQCKIAECA
                     TKQEGADLIVSTTILPTTYNIPAIKATAYITGVNTAALDKKILDALK"
     gene            1102..2376
                     /locus_tag="ACGI59_002667"
     CDS             1102..2376
                     /locus_tag="ACGI59_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002843259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HFG9740448.1"
                     /translation="MDKLLAVVQYILNMGPTVILPITIVVLGMIFRIKFTKALKSGLT
                     IGIGFVGINLVVNLLTSTLGPAAQGMVERFGLNLTVIDVGWPTTASATWASPVAPILI
                     PVCLLVNLLLLYFNKTKILNVDIWNYWHFIVAGAVGYIVTGSYVWAIFCAILMELLVL
                     FIAEKTAKNVQEFYDLEGIALPTGSTASFAPLGYLVGKFVEKIPGINKIQADPDSIQK
                     RFGIFGEPMMMGIILGIILGALAGYDVAGIFNVGISMGAVMLLMPRMVRILMEGLIPI
                     SESIRDFLQAKYGDRELYIGLDAAVATGHPAVISTALILVPITLFLAVILPGNKVLPF
                     GDLATIPFYMAFIVCFRKGNIVQSVITGTIVIAISLYMATNFADVHTQLLQQANMMPE
                     GTTMVTSIDTGGNLLKWGILKLSQLVSSFGIF"
     gene            2506..3558
                     /locus_tag="ACGI59_002668"
     CDS             2506..3558
                     /locus_tag="ACGI59_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFG9740449.1"
                     /translation="MKAAVLHGTNDMRFEDIEIKPCESDEVKIKVMAAGICGSDPPRV
                     LKHWKYPVPAIPGHEFSGVIAEVGKDVKNVKVGDRVVAIPFIPCNECEYCKRGLFSLC
                     DDHGMLGAKSFGAFAEYVNIKATNVLPIGDMNFEDAAMIEPLAVAMHGVLNIGVQVGD
                     TVAVMGSGTMGQLVIQGLKIAGAGTIIAVDISDNKLRESKELGADIIINAKDINPVEK
                     IKELTGGKGVDIALECAGSKITQEQCLLITKKKSKIGFLGIAYSDITLSEEAFENIFR
                     KELELKGFWNSYSAPFPGQEWTKGINLVNEGKIKLKEMVSHRFSLEDTYKAFEMIRDR
                     KEEFNKILILPQGVEK"
     gene            3558..4589
                     /locus_tag="ACGI59_002669"
     CDS             3558..4589
                     /locus_tag="ACGI59_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFG9740450.1"
                     /translation="MKAVVKTKPGYDNVEVLEVEEPKATGDKVKIKVEYSGICGSDIH
                     SFKGEYANIKAPVTLGHEFSGIVVEVGEDVKNIKVGDRVTSETTFATCEKCIYCETKD
                     YNLCPTRKGIGTQVNGSFAEYVLSREESIHVLPENVSLLSAALTEPLACCVHAALEKT
                     TIESSDTVLIIGPGPIGLLLAQVVKSQGATVIMSGVTKDKERLEIAKNLGVDRTVNVM
                     EESLADVVNEMTDGNGVNKGFDCSGFMPAVNEALRLLRKKGTFVQVGIFAKKLNEMDQ
                     EAIIQRELQYIGSRSQKPSSWITALDLMANGKVNTEALVTRTVGLDEFKEGITALMNG
                     EEIKVAVKS"
     gene            4681..5493
                     /locus_tag="ACGI59_002670"
     CDS             4681..5493
                     /locus_tag="ACGI59_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFG9740451.1"
                     /translation="MSTLRDHSNNIRKNIIKMVAEAKSGHPGGSLSIADMLTVLYFDK
                     MNVCAENPKMADRDRFVLSKGHAAPALYATLAEKGFFPEEELITLRKFGSKLQGHPDM
                     KKVAGIDMSTGSLGQGLSAANGMALAGKLDNKDYTVYTILGDGEIQEGQIWEAAMTAS
                     HYKLDNLIAFVDLNGLQIDGSNEEVMNVSPVDDKFKSFGWNVIVINGHSFEEIGNAVD
                     EAKKVTGKPTVIIAKTVKGKGVSFMENNAAWHGTAPNAEQAEQALKDLEGGL"
     gene            5496..6416
                     /locus_tag="ACGI59_002671"
     CDS             5496..6416
                     /locus_tag="ACGI59_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HFG9740452.1"
                     /translation="MGIATREAYGQVLKELAENKDIVVLDADLGKATKSISFKEVAPD
                     RFFDMGIAEGDMIGTAAGLATCGKIPFASTFAIFAAGRGYEQIRNSVAYPNLNVKIAA
                     THAGVTVGEDGGSHQAIEDISLMRGIPNMVVLNPADALEARQAILTSIDYNGPVYIRL
                     GRAATPDVNSENYKFEIGKGTVLREGSDITVIATGIMVAKALEAAEELAKEGINVEVV
                     NISTIKPLDEALIKETAKKTGKVVTAEEHSIIGGLGSAVCEALAETKDVVVRRIGVKD
                     VFGQSGTPADLLKHYGLTTEDIVKNIKELL"
     gene            6591..7037
                     /gene="rpiB"
                     /locus_tag="ACGI59_002672"
     CDS             6591..7037
                     /gene="rpiB"
                     /locus_tag="ACGI59_002672"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HFG9740453.1"
                     /translation="MKISIGCDHGGYELKEYIKENLINKGIEVEDVGTNSKESVDFPI
                     YAKKVSQNVQSKKSDLGILCCGTGIGMSIAANKFKGIRAAVVSDCFSAKATREHNNTN
                     VLCLGERVIGKGLAMRIVEEWLNSSYNGERHARRLQMIDDIEKENL"
     gene            7067..7732
                     /locus_tag="ACGI59_002673"
     CDS             7067..7732
                     /locus_tag="ACGI59_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFG9740454.1"
                     /translation="MSIICASIMCADQMKLKEELEALEEAGVKLLHCDVMDGIFVKNL
                     AMGPELLKSISENTTIPLDIHLATETPDKYIDMMSYIKPKYISFHVESSTNVKADIQK
                     LRNYGIGPVLAISPQTSVDKIEEYISLVEGILVMTVNPGFAGQKFNLSVLDKLDKLTE
                     ILKDYDNPPFIEVDGNINKDTIKLMNGKKVDIYVVGTSALFNDKPPISYKDKIEELKE
                     SIK"
     gene            7842..9344
                     /locus_tag="ACGI59_002674"
     CDS             7842..9344
                     /locus_tag="ACGI59_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HFG9740455.1"
                     /translation="MGDLYKLQNGTDIRGIAYENDSKEVNLTVEEVKKIAKAFHIWLK
                     EKTKKDKVTVAIGTDSRITGSQFRSTVIETLTNDDCNVIDCEIATTPAMFMTTIMDGY
                     NSDGAIMITASHLPYYYNGLKFFTESGGLEKTDIKEMLDIAVKNDSVDYEEVNKKGEV
                     IVKNLIEDYSNLLIDKIRKGVNSSKNYEKPFSGLKILVDAGNGAGGFFAEKVLHILGA
                     DTTGSQFLNPDGMFPNHIPNPENKEAMESICKAVLDNKSDLGIIFDTDVDRAAIVGKN
                     GKPINKNALIAVISSIVLEEHPKTAIVTDSITSEGLAKFINELQGRHHRFKRGYKNVI
                     NEAIRLNSEGEECHLAIETSGHAALKENYFLDDGAYLIAKILIKVAKLSLEGKTIEEL
                     IENLEEAQEEKEVRIGINKEDFRPYAEDILKDLDSHVKSIDGWSVAPNNFEGIRVNCD
                     ESNGDGWFLLRISLHEPLLALNIESNKIGGAEQIYSKLKLFLEKYDLKGL"
     gene            complement(9656..11248)
                     /locus_tag="ACGI59_002675"
     CDS             complement(9656..11248)
                     /locus_tag="ACGI59_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HFG9740456.1"
                     /translation="MVTKLKNLIINDSKAKFILLLLLFLLVSILFEAVYDLNNNFSYN
                     INYYYKEFALPYFYFFGTPVHLSKVELEFVCIGIILSSFLLTILCFTLYKKNKQTKVK
                     FLENVISLFSKVKIELTLILTLILLTLRFHAFTSNKMFYNTNLIIYNFIIISIVYIIY
                     ENIKYNNYNLKDMSITYVFFSNLLNMYNKKTINKKLAIVFLISIVVQAIIQYLVLIFF
                     MYIYYAEAIAIVILFTLVNTVFHFYIYNFLSVKFNYINNISKNIKNIENGNLKYKLEV
                     IGDDEISTLAKSINNITGGLETAVDANIKNERMKAELITNVSHDLKTPLTSILSYVDM
                     LKNNDFDRETINDYINILDKKSQRLKLLIDDIFEASKLSSGDVEFNITKTDIRELLIQ
                     SIVEFEDKIQNSSLDFIIETPEYAVFTMIDGKKTWRVFENLICNILKYSMPNTRVYID
                     MFIKEENIILTFKNISNDKLNLKPEELIERFRRGDISRKTDGSGLGLSIAQNIIELEN
                     AHMEIIIDADLFKVMLTFRRVN"
     gene            complement(11217..11933)
                     /locus_tag="ACGI59_002676"
     CDS             complement(11217..11933)
                     /locus_tag="ACGI59_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFG9740457.1"
                     /translation="MNQNILVVDDDREIVDAIEFYLRPDNFNVLKAYDGLEALDILIE
                     NDIKLIIMDVMMPNLDGLKTTLKIREKRNIPIILLSAKSEDMDKIIGLNMGADDYITK
                     PFNPLELTARVKSQLRRYINLGDFSNTNNSNDTNYILKSGGLELNTDTKIVSLDGEEI
                     KMTPLEYKILTLLMSRKGKIFSSHEIYENVWNEDAYSCERTIAVHIRRIREKIEINPK
                     EPKYLKVVWGIGYKIEKLNY"
     gene            complement(12473..13273)
                     /locus_tag="ACGI59_002677"
     CDS             complement(12473..13273)
                     /locus_tag="ACGI59_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HFG9740458.1"
                     /translation="MTFKNKKFTINKYKILSIILVLCLILTAFSSYVNYSHKKFKDYV
                     VFYVQHQDDEVLWAGSAIVNAIKERGKNHVFVVLVSTGCGISVFDKYKKYENLTNIQK
                     SEYRNREFLASLNSLGVIRENIILLPETNTNGNTDFNYMEKIALTFEEKFKNVTHIAH
                     TYKLDDHLQHLKNGSVIQNLYNAGKIKDAKYFVKPKFADKITFNNKIVYKAHSTEDYE
                     KVKNACQQYKIVDELEYREGIGYKSDHKSFDKLLNNKDVPAILHTPNL"
     gene            complement(13587..14318)
                     /locus_tag="ACGI59_002678"
     CDS             complement(13587..14318)
                     /locus_tag="ACGI59_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF364 domain-containing protein"
                     /protein_id="HFG9740459.1"
                     /translation="MNIFDILRDYLLVQVDKYNLNMDMISIVSKSLSSKEAIGNTKRK
                     DFPIIVGKEIMLEADFKGAKGQAFTSTPSTFEGSLKDILSLDLHDNPHDRSLFIASLN
                     AVMKYLGKTDRTIHCKNNEPEICAKKFPEFIKMEFGNPKVAIIGYQPAIIDNIKDFFE
                     TRVLDLNPEFVDTIQYNVKIEDGIRDYEDVISWADLVICTGSTLCNNSIINFLSLNKP
                     VYYYGTTIAGASNILGLKRLCFCSK"
     gene            complement(14305..15000)
                     /locus_tag="ACGI59_002679"
     CDS             complement(14305..15000)
                     /locus_tag="ACGI59_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFG9740460.1"
                     /translation="MLKIKNLSLDLPNFKLQENSLNINNHEYFVLLGASGSGKTLFLE
                     TLAGRYPSAKGNVSYKENLISNLPPENRNIGFVYQNFELFPNMNVEDNIRFPLKLRKI
                     PIDEQILKANHLSKLMQIEDIKNRYPKNLSGGEKQRVAFARALIAEPDILLLDEPMSS
                     LDYITKKHISKILKDVYIKYKPTVIHVTHDISEAMFFAHKIGIMNNGKINHIFDLNEE
                     IKNKGESFFYEYI"
     gene            complement(15002..15793)
                     /locus_tag="ACGI59_002680"
     CDS             complement(15002..15793)
                     /locus_tag="ACGI59_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9740461.1"
                     /translation="MKNKFFTTLLTGFFAIVFLFIVSPLLMLIIKGISYVPICLKSVE
                     VQYAIILSIKTSLISTIVCLLLAIPVAYFLHITKLPFKKLIIQIINLPMSLPHLVSGI
                     ALLLLFGRMGIGDSIYKIFKLDFIFTKQGIVLAQVFVNLPLTIKILYTSLNESNEKMI
                     FVARTLGCNSWEAFRFIILPNLKTGIISATVMTWSRALGEFGAVAMIAGSTRMKTEII
                     PTSIYLNMSTGDIDIAIGIAVILIFISLTCLMLFEIFFNREVDKN"
     gene            complement(15803..16615)
                     /gene="modA"
                     /locus_tag="ACGI59_002681"
     CDS             complement(15803..16615)
                     /gene="modA"
                     /locus_tag="ACGI59_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFG9740462.1"
                     /translation="MFKKLSVLALAMIISLSITACNSKENKANDESNTKVTTADDSKE
                     KETLFVYSGAGLKKPMDEIAKKFEKENNVKVEYSYAGSAQLIAQIETSHKGDVFIVGS
                     EPIYSKAFEKELVGEHKTVAHHTPAIVVPKGNPANIQKLEDLNKDGVKLILGDKESNA
                     IGKTTQKILEKNNLKSINDNVVSTAATVNEMIVQLTSSKADATIATKDSVFGNEDVEV
                     IEIAQDKNIDQLISAGIVNYSDKKDLANKFINYIESDEAKQIFKKYGFEPVK"
BASE COUNT         6380 a         2062 c         2699 g         5616 t
ORIGIN      
        1 tctaacaaaa aaaaccacaa aaaatataaa aaagtatggc tagttaagtg attaacaagt
       61 aaaaattaaa ataaaaatca aaaaaaattt atcaatgaaa gtcagctaaa atcctggaag
      121 agagcattat ttttgggaaa acattttctt ttgtttcaca aaaaaagcca ctttttggca
      181 cacaacatgc tttatataaa gtcaagaaaa actaaggtat aagacaaagg caattaggag
      241 gtgtaaatat ggagtttata aattttgtaa gtgataaagt tatagacctt aaaatggatt
      301 gtaaaacgaa agaagatttc tttaaagaaa tacataataa agtttttgat ttaggctttg
      361 taaaagaaga atttggagaa aaaatattag caagagaaaa tgtatttcca acaggtttaa
      421 atttaggtga ttatggagta gcaataccac atacagatgc ggaatatatt aaagagcaat
      481 ttatatcagt ctgtactttt aatgaaccag tagtatttag ttctatggaa gaccaagatg
      541 aaaaagtacc tgtaaatttg gctttcgtat taggacttaa tcaaccacac agtcaattat
      601 ctgtattaac agaattaatg ggaattatgc aaaataaaga gttagttgat aagttgatga
      661 gttctactga taaagaagaa gttttaaaga cactaaagtc tttataaaaa taaaaaagta
      721 ctaaaaataa aaaatataaa aataaaaatt attaggagga aataaaaatg aaaaaaatat
      781 tagtagcatg tggagcagga atagcaacat caacagtagt ttgtgacaag gtagagagat
      841 tagttaagga aaataatata caagcagaag ttatacaatg caaaatagct gaatgtgcaa
      901 caaagcaaga aggagcagat ttaatagtat caacaactat actaccaaca acttataata
      961 taccagcaat aaaagcaaca gcatatataa ctggagtaaa tacagcagca ttagataaaa
     1021 aaatattaga tgctttaaaa taatcctatc atagatacat tgaaataata ttttataaaa
     1081 cagaaaaagg aggatttata aatggataaa ttattagcag tagtacaata cattttaaat
     1141 atgggaccga cagtaatact accaataaca attgtagtac taggaatgat atttagaatt
     1201 aagtttacaa aagctttaaa gtcaggtctt acaattggta taggatttgt gggtataaac
     1261 ttagttgtaa acctattaac atcaacacta gggccagcgg ctcaaggaat ggttgaaaga
     1321 ttcggactta atttaacagt aatagatgtt ggttggccaa caacagcatc agcaacatgg
     1381 gcttcaccag ttgcaccaat attaatacca gtttgtttat tagtaaactt actattatta
     1441 tacttcaata aaacaaagat attaaacgtt gatatatgga attattggca ctttatagta
     1501 gcaggtgcag ttggatatat agtaacagga agttatgttt gggctatatt ctgtgcaata
     1561 ctaatggagt tattagtact gtttattgca gaaaagacag caaaaaatgt tcaggagttt
     1621 tatgatttag aaggtatagc attaccaact ggttcaacag cttcatttgc tccacttgga
     1681 tatttagtag gtaaatttgt tgaaaaaata ccaggaataa ataaaataca agcagaccca
     1741 gactctatac aaaaaagatt tggtatattt ggagaaccaa tgatgatggg gattatacta
     1801 ggtataatac ttggagcact tgcgggatat gatgtagcag gaatattcaa tgtaggtata
     1861 tcaatgggtg cagtaatgtt acttatgcct agaatggtta gaatattaat ggaaggttta
     1921 attccaattt cagaatcaat aagagatttc ttacaagcaa aatatggaga cagagaactt
     1981 tatataggtc tggatgcagc agtagcaact ggacatccag cagttatatc aacagctctt
     2041 atacttgttc ctataacttt gttcttagca gttatactac caggaaacaa agttttacca
     2101 tttggagact tagcgacaat accattttat atggcattta tagtgtgttt tagaaaaggt
     2161 aatatagttc aatcagttat aacaggaaca atagtaatag ctatatcact ttatatggct
     2221 acaaactttg cagatgttca tacacaatta ttacaacaag caaatatgat gccagaagga
     2281 acaacaatgg ttacaagtat agatacaggt ggaaacttgc taaaatgggg aatattgaaa
     2341 ttatctcaat tagtttcttc atttggaata ttctagtatg tagttaatag agattactct
     2401 tttttagtaa agtatgcgaa aatttaacat ttagcagcta ctaaaaaaga gtaatatttt
     2461 ttataaattt ttaaaaatac aaaatacaag aggaggagat ttatcgtgaa agcagcagta
     2521 ttacatggca caaatgatat gagatttgaa gatatagaaa taaaaccatg tgaaagtgat
     2581 gaagtaaaaa taaaagttat ggcagcagga atatgtgggt cagatcctcc aagagtatta
     2641 aaacattgga aatatccagt tccagctata ccaggacatg agttttctgg agtaattgct
     2701 gaagttggta aggatgtaaa aaatgtaaaa gttggagata gagtagttgc aattcctttt
     2761 ataccttgta atgaatgtga atattgtaaa agaggattat tttcattgtg tgatgaccat
     2821 ggaatgttag gagctaaaag ttttggagct tttgcagagt atgtaaatat aaaagctaca
     2881 aatgtacttc caataggaga tatgaacttt gaagatgctg caatgataga gccactagca
     2941 gttgctatgc atggagtact taatataggt gttcaagttg gagacactgt agctgtaatg
     3001 ggtagtggaa caatggggca gttggtaata caaggactta aaatagcagg ggcaggaaca
     3061 attatagcag ttgatatatc tgataataaa ttaagagaat caaaggaatt aggggctgat
     3121 ataataatta atgctaaaga tattaaccca gtagaaaaaa taaaagaact tactggtgga
     3181 aaaggtgttg acatagctct tgaatgtgca ggttcaaaaa taactcaaga acaatgttta
     3241 cttataacta aaaagaaaag taagatagga tttttaggga tagcatattc agatataact
     3301 ttatcagaag aagcatttga aaatatattt agaaaagaac tagaattaaa aggtttttgg
     3361 aactcttatt ctgcaccatt tccaggacaa gaatggacta agggaatcaa tctagttaat
     3421 gaaggaaaga taaaattaaa agaaatggta tctcatagat tctcacttga agatacttac
     3481 aaagcatttg aaatgataag agatagaaaa gaagaattta acaaaatatt aattttacca
     3541 caaggggttg aaaaataatg aaagcagtag ttaaaactaa accaggctat gataatgtag
     3601 aagttttaga agtagaagaa ccaaaagcta caggagacaa ggttaaaata aaagtagaat
     3661 atagtggtat atgtggttca gatatacatt catttaaagg tgaatatgca aatataaaag
     3721 ccccagtaac attaggtcat gaattttctg gaattgttgt agaagtagga gaagatgtta
     3781 aaaatataaa agtaggagat agagttacat cagagactac ttttgcaact tgtgagaaat
     3841 gtatatattg tgaaactaaa gattataact tatgcccaac tagaaaaggt attggtactc
     3901 aagtaaatgg aagttttgct gagtatgtat tatcaagaga agaaagcata catgtacttc
     3961 ctgaaaatgt atctttatta tcagcagccc ttacagagcc actagcttgt tgtgttcatg
     4021 cagctcttga aaagactact atagaaagtt ctgatacagt tttaataata ggaccaggac
     4081 caattggttt attattagct caagttgtta aatctcaagg tgctacagtt ataatgtctg
     4141 gtgttacaaa agataaggaa agattagaaa tagctaaaaa tcttggagta gatagaactg
     4201 taaatgtaat ggaagaaagt ttggcagatg tagtgaatga aatgactgat ggaaatggag
     4261 taaacaaagg atttgattgt tctgggttta tgccagcagt taacgaagcc ttaagactgc
     4321 ttagaaaaaa aggtactttc gtacaagttg gaatctttgc taaaaaatta aatgaaatgg
     4381 accaagaagc aataatccaa agagagttac aatatatagg aagcaggtct caaaagccaa
     4441 gttcttggat tacagcatta gatttaatgg caaatgggaa agtaaataca gaagctttag
     4501 ttactagaac tgttggttta gacgaattta aagaaggtat tacagcttta atgaacgggg
     4561 aagaaataaa agtagcagta aaatcttaaa aaaacatttg tattatttta aaaaaaattg
     4621 taaaatctac tgtaggataa aaaaatgtaa ggtcttaaaa aataacagga ggaagaaagt
     4681 atgagcactc ttagagatca ttctaataat attagaaaaa acataataaa aatggttgca
     4741 gaagctaaaa gcggacatcc aggtggttca ttatcaatag ctgatatgtt gacagtatta
     4801 tattttgata agatgaacgt atgtgcagaa aatccaaaaa tggctgatag agatagattt
     4861 gttctatcaa aaggtcatgc agcaccagct ttatatgcta cattagcaga aaaaggattt
     4921 ttcccagaag aagaattaat aacactaaga aaatttggtt ctaagttaca aggtcaccca
     4981 gacatgaaaa aagtagcagg aatcgatatg tctactggtt ctcttggtca agggctatct
     5041 gcagcaaatg gtatggcact tgcaggaaaa ttagataaca aagactatac agtatatact
     5101 atattaggtg atggagaaat ccaagaaggt caaatatggg aagctgcaat gacagcatca
     5161 cattacaaat tagataattt aattgcattt gttgacttaa atggattaca aatagatggt
     5221 tctaatgaag aagtaatgaa tgtaagtcca gtagatgaca aattcaaatc ttttggatgg
     5281 aatgtaatag taataaatgg acattctttt gaagaaatag gaaatgcagt tgatgaagct
     5341 aaaaaagtta caggaaaacc aactgtaata atagctaaaa cagtaaaagg taaaggtgtt
     5401 tcatttatgg aaaataatgc tgcatggcat ggaacagctc caaatgcaga acaagcagag
     5461 caagcattga aagatttaga aggaggtcta taggaatggg aatagcaact agagaagctt
     5521 acggacaagt gttaaaagaa cttgcagaaa ataaagatat agtagtatta gatgcagatt
     5581 taggaaaagc tacaaaaagt atatcattta aagaagttgc cccagataga ttttttgaca
     5641 tggggatagc agaaggtgac atgattggta cagcagcagg tcttgctact tgtggaaaaa
     5701 taccttttgc gagtactttt gcaatatttg cagcaggaag aggctatgag caaataagaa
     5761 actctgtagc atatccaaat ttaaatgtaa aaatagcagc tactcatgca ggtgtaacag
     5821 taggtgaaga tggtggtagc catcaagcta tagaggatat atctctaatg agaggtatac
     5881 caaatatggt tgtattaaat ccagcagatg ctttagaagc aagacaagct attcttacat
     5941 ctattgatta taatggacct gtttatataa gattaggtag agctgcaact cctgatgtaa
     6001 acagtgaaaa ttataaattt gaaataggaa aaggaactgt tttaagagaa ggttctgaca
     6061 taacagtaat agctacagga ataatggtag caaaagcctt agaagcagca gaagaacttg
     6121 ctaaagaagg tataaatgta gaagttgtta atatatctac aataaaacca ttagatgaag
     6181 cattaataaa agaaactgct aagaaaactg gaaaagtagt tacagcagag gagcacagta
     6241 taataggtgg tttaggttca gctgtatgtg aagctttagc agaaactaaa gatgtagtag
     6301 ttagaagaat tggtgttaaa gatgtatttg gtcaatcagg tacaccagct gatttattaa
     6361 aacactatgg tttaactaca gaagatatag taaaaaatat aaaagagttg ttatagatat
     6421 aaatatatct aaataacaaa ttgatttgac ataagtcaat taaagctgtc ttaaaaatag
     6481 aaagaaattt ctacaatggg acagctttat ttttacaata ctttattttt atgataatta
     6541 taaaatagtc tgaaagtaat caaaaaaatt ataaaaagag gtgctattta atgaaaatat
     6601 ctattggatg tgaccatggt ggttatgaat taaaggaata tataaaggaa aatttaataa
     6661 ataaaggtat agaagttgaa gatgtgggta caaattcaaa agaaagtgta gattttccaa
     6721 tatatgcaaa aaaggtaagt caaaatgttc aaagtaaaaa atctgattta ggcatacttt
     6781 gttgtggaac aggaataggt atgtctattg cagcaaataa gtttaaagga atcagagcag
     6841 ctgttgtttc tgactgtttt tcagctaagg caactagaga acacaataat acaaatgtac
     6901 tttgtttagg agaaagagtt attgggaaag gattagcaat gagaattgtt gaagaatggt
     6961 taaattcaag ttataatgga gaaagacatg caagaagatt acaaatgatt gatgatatag
     7021 aaaaagaaaa tttataaata tataatttat aacaaggggg agcgttatgt ctattatttg
     7081 tgcttcaata atgtgtgcag accaaatgaa gttaaaagag gaattagaag ctcttgaaga
     7141 agcaggtgta aaattacttc attgtgatgt tatggatgga atatttgtta aaaacttggc
     7201 tatgggacca gagttattaa aatcaataag tgaaaataca acaatacctt tagacataca
     7261 cttagccaca gaaactccag ataaatatat tgatatgatg tcttatataa agccaaaata
     7321 tatttctttt catgtagaat caagtacaaa tgtaaaagct gatatacaaa agttaagaaa
     7381 ttatggtata gggccagtgt tagcaataag ccctcaaaca agtgtggata aaatagaaga
     7441 atatataagt ttagttgagg gtatcttagt aatgacagta aatccagggt ttgcaggtca
     7501 aaagtttaat ctttctgtat tggataagtt ggataaactt acagagatac taaaagatta
     7561 tgataatcct ccatttatag aggttgatgg aaatataaat aaggatacta ttaaacttat
     7621 gaatggtaaa aaagtagata tatatgttgt tggtacttca gctttattta atgataagcc
     7681 tccaatttca tataaagata aaatagaaga actaaaagaa agtataaagt aaccatgtaa
     7741 taaaattata atatgtttct gtttaatagt aaaaatttag gtggaaacaa gaaattttat
     7801 aatatttaat aaaaattttg gttgagtaag gagaattttt tatgggagac ctatataaac
     7861 tacaaaatgg tactgatatt agaggaatag cgtatgaaaa tgattctaaa gaagttaacc
     7921 taactgtaga agaagtaaaa aaaatagcta aagcgtttca tatttggtta aaagaaaaga
     7981 ctaagaaaga taaagtaact gttgccatag gaactgattc taggataaca ggaagccaat
     8041 ttagaagtac tgttattgaa actttaacta atgacgattg taatgtaatt gattgtgaga
     8101 ttgccacaac tccagctatg tttatgacaa ctattatgga tggatacaat tctgatgggg
     8161 cgattatgat tacagcaagt cacttacctt attactataa tggtttaaag tttttcacag
     8221 aaagtggagg tttagaaaaa actgatataa aagagatgtt ggatatagct gttaaaaatg
     8281 atagtgtaga ctatgaggaa gtaaataaaa aaggtgaagt tatagtaaaa aatttaatag
     8341 aagattattc taatttattg atagataaaa ttagaaaagg tgtaaattca agtaaaaatt
     8401 atgaaaagcc tttttctggg cttaagatat tggttgatgc aggaaatggg gctggtggat
     8461 tctttgctga aaaagtttta cacatactgg gagcagatac gacaggtagt cagtttttga
     8521 acccagatgg tatgttccca aatcatatac ctaatcccga aaacaaagaa gcaatggaaa
     8581 gcatatgtaa agctgtgtta gataataaga gcgacctagg aataatcttt gatacagatg
     8641 tagacagagc tgctattgta ggtaaaaatg gcaaaccaat aaataaaaat gctcttatag
     8701 ctgttatatc atcaatagta ttagaagaac atccaaaaac agctatagta acagattcga
     8761 ttacttctga aggacttgct aaatttataa atgaattaca aggaagacat catagattta
     8821 aaagaggata taaaaatgta ataaatgaag ctataagatt gaatagtgaa ggtgaagagt
     8881 gccatttagc tatagaaacg tctggacatg ctgctttaaa agaaaattat ttcttagatg
     8941 atggggcata tttaatagct aaaattttaa taaaagtagc aaagctatcc ttggaaggaa
     9001 agactataga agaattgata gaaaaccttg aagaagctca agaagagaaa gaagttagaa
     9061 taggcattaa taaagaagat tttagaccat atgcagagga tatattaaaa gatttagatt
     9121 ctcatgtcaa gagtattgat ggatggtctg tagcaccaaa taattttgaa ggcataagag
     9181 ttaattgtga tgaatcaaat ggagatggat ggtttttact tagaatatca ctacatgaac
     9241 cattattagc tttaaatatt gaatcaaata aaattggcgg agcagaacaa atttattcta
     9301 agttaaaact tttcttagaa aaatatgatt taaaaggtct ttagggtaaa tagaaagaat
     9361 atatttatat tgaagaataa atgtaaattt gtacctaaga ttacaaatat tacaaaattg
     9421 taatcttagg taacagagat gtaactgatt atggaaacac tatgtgatat tatagataca
     9481 gagagatgaa atatgtgatg aattatagag aaatataatt taaaatgtat taatatcaca
     9541 ccaaaaaggt ataatcaaaa aatatgtaaa tataaacccc aacatttaga agaagacctc
     9601 taaggaggtt tttttcttgt ttaaataact taaaatatat ttttatatca atatatcaat
     9661 taactcttct aaaagtaagc attaccttaa ataaatcagc atctataatt atttccatgt
     9721 gtgcattttc taattcaatt atattttgag caattgataa accaagacca gagccatctg
     9781 tttttctaga tatatcaccc cttctaaatc tttctattaa ctcttctggc tttagattta
     9841 gtttatcatt tgaaatattt ttaaacgtta atattatatt ttcttccttt ataaacatat
     9901 ctatgtagac cctagtgtta ggcatagagt attttagtat attgcaaatt agattctcaa
     9961 aaactctcca agttttttta ccatctatca tggtaaaaac agcatattca ggtgtttcta
    10021 ttataaaatc taaactgcta ttttgaattt tgtcttcaaa ctctacaata ctttgaatta
    10081 aaagttctct tatatcggtt tttgttatat taaattctac atctccacta cttagcttag
    10141 atgcttcaaa tatgtcatct attaataatt ttaatctttg tgatttttta tctaaaatat
    10201 ttatatagtc atttatagtc tctctgtcaa agtcattatt ttttaacata tcaacatagc
    10261 ttaatataga tgtaagtgga gtttttaagt catgagatac gtttgtgata agctctgcct
    10321 tcatcctttc attttttata ttagcatcta cagcagtttc aagaccacct gttatattat
    10381 ttatactttt ggctaaagta gatatttcat catctcctat aacttctaat ttatacttta
    10441 aattgccatt ttctatattt tttatatttt ttgatatgtt atttatgtaa ttaaacttaa
    10501 cagataaaaa gttgtatata taaaaatgaa aaactgtgtt tacaagtgta aataaaatta
    10561 ctatagctat tgcctcagca taatatatat acataaagaa tatcaaaaca agatattgga
    10621 taatagcttg tacaactata gatattaaaa atactatagc taatttttta tttatagttt
    10681 ttttattata catattaagc aagtttgaaa aaaatacata tgttatagac atatctttta
    10741 gattatagtt attatattta atattttcat atattatata gactatagat ataattataa
    10801 aattgtagat tattaaatta gtgttgtaaa acattttatt acttgtaaat gcatgaaacc
    10861 ttaaggttaa tagtatcaaa gttaatataa gtgtaagttc tatttttacc ttagaaaaaa
    10921 ggcttatgac attctctaag aatttgactt tagtctgttt atttttttta tacaatgtaa
    10981 aacatagtat agtcaataaa aaacttgata atataatacc tatgcagaca aactctaatt
    11041 ctacttttga caaatgtaca ggagtaccga aaaaataaaa ataagggaga gcaaactctt
    11101 tataataata attaatatta taagaaaagt tattattcaa atcataaact gcttcaaata
    11161 atatagaaac taatagaaac agtaataata ataaaataaa ttttgcttta gaatcattaa
    11221 taattaagtt tttcaatttt gtaaccaatg ccccacacca cctttaaata ttttggttct
    11281 tttggattaa tttcaatctt ttctctaatt cttctaatat gaacagcaat agttctctca
    11341 caactataag catcctcatt ccagacattt tcataaatct catgagaaga gaaaatttta
    11401 ccttttcgtg acataagaag tgtaagtatt ttatattcaa gtggtgtcat tttaatttct
    11461 tcaccatcta aagaaactat ttttgtatct gtatttagct ctaggccacc actttttaga
    11521 atataattag tatcatttga gttatttgta ttactaaaat cacctaaatt tatatacctt
    11581 ctaagttgag attttactcg agcagtaagt tctagtggat taaatggttt agttatatag
    11641 tcatcagcac ccatattaag acctataatt ttgtccatat cttctgattt tgcagataag
    11701 agaattattg gtatattcct tttttctctt attttaagtg tagttttaag cccatctaaa
    11761 ttgggcatca ttacatccat tattattaac ttaatatcat tttcgattag tatgtccaat
    11821 gcttcaagtc catcatatgc ctttaaaaca ttaaagttat caggtctcaa atagaactct
    11881 atagcatcaa ctatctctct atcatcatct acaaccaata tattttgatt caacttatta
    11941 acccccttga aattttattt ttaaccaatt tttattttat accaaaaaga ggtattagaa
    12001 aacagtaagc ccgttcatta tgaaataaaa actagtactt ttaatatatt gtcaatgcat
    12061 atagtaaatt taaaagtata gacgcccata ttaatctgaa ggtaaaagtt tatgaaaata
    12121 tctagggtaa tctatcacac tccttatgtt attttggttt attaattttt taggtttaaa
    12181 catatattaa caaatataaa taacaaatga gtttcataat tattaagaat ttataacaaa
    12241 tatttatttt aatgtagttt aatataaata ggtagcttaa tgtagatatg tataaagcat
    12301 ttaataaggc ttataggttt taatgatgat atctttatat attttaagag atgatgaaat
    12361 tagatatggg taatagttgt atgagaaaaa gtagtatagc acaaaaagtg ctatatcaca
    12421 aaatctcaaa taatttcctt tttataactt aaattaaaag aataaatatt tcttataaat
    12481 taggtgtatg aagtatagca ggaacatctt tattatttaa taacttatca aatgatttat
    12541 ggtcagactt atagccaata ccttctctat attctaattc atcaacaatt ttgtattgtt
    12601 gacaggcatt tttaaccttt tcatagtctt cagtagaatg agctttataa actatcttat
    12661 tattaaaagt tattttatca gcaaatttag gttttacaaa atatttagca tcttttattt
    12721 taccagcatt atataaattt tggataacag aaccattttt taaatgttgt aaatggtcat
    12781 ctaacttata agtatgtgct atatgagtta catttttaaa tttttcttca aatgtaagag
    12841 caattttttc catgtaattg aagtcagtat taccatttgt atttgtttct ggtaataata
    12901 ttatattttc ccttattaca ccaaggctat taagagaagc taaaaattct ctatttctat
    12961 attctgattt ttgtatattt gttaggtttt catatttttt atatttatca aagacggata
    13021 taccacatcc agtagatact aaaaccacaa atacatgatt ttttcctctt tcttttatag
    13081 cattaacaat agcactccca gcccacaaca cttcatcatc ttgatgctga acatagaaga
    13141 caacataatc tttaaatttt ttatgactat aattcacata gctactaaaa gcggttaaaa
    13201 tcaaacataa aactagaata atagatagaa ttttatattt atttatagta aattttttat
    13261 tcttaaatgt catttaatca tctcctgaaa agttgtctta gttatattat caaacaaagt
    13321 actaaaaatt atttcttaaa tgctgaaaaa tatagagtag attaatggaa taaaattttt
    13381 ttattagttt aaaataaaga aaacatttat gtataagact aatcaaaata ttaatgattg
    13441 ataggaattg agatttaaag ggattgtaca tttattcggg attcatttta atagaatttt
    13501 actttagtac ttagaataaa agtaataact ttttaaattt aaagagtaca catatatatt
    13561 tgtgtactcc ttgatgatat gattgcttat ttactacaaa aacagagtct ttttaaacct
    13621 aaaatatttg atgcaccagc tatcgtagtt ccataatagt aaactggttt gttaagtgat
    13681 agaaaattta taattgagtt attacataat gtacttcctg tgcaaatcac taaatcagcc
    13741 caagatatta catcttcata atctcgtata ccatcttcaa tcttaacatt atattggata
    13801 gtatccacaa attcaggatt taaatctaat actcttgttt cgaaaaagtc cttaatatta
    13861 tcaattatgg ctggttgata tcctataatt gctacttttg gattaccaaa ttccattttt
    13921 ataaactcag ggaatttttt tgcacatatc tctggctcgt tatttttaca atgaatggtt
    13981 ctatccgtct tgcctaagta tttcataact gcatttaatg atgctataaa aagacttcta
    14041 tcatgaggat tgtcatgtaa atctaatgat aatatgtctt ttaaagaacc ttcaaatgta
    14101 gatggagtag aagtaaatgc ttgtccttta gctcctttaa agtcagcttc aagcattatt
    14161 tctttaccaa ctataattgg gaaatcttta cgcttagtat ttccaattgc ttcttttgaa
    14221 gatagagatt tggacacaat acttatcata tccatattta aattatattt atcaacttgt
    14281 actaatagat aatctctcaa aatatcaaat atattcatag aaaaaagatt ctcctttatt
    14341 ttttatttct tcatttaaat caaaaatatg atttattttt ccattgttca ttattcctat
    14401 cttatgagca aaaaacattg cttcagaaat atcatgagta acatgaatca ctgttggttt
    14461 gtattttatg tagacatcct tcaaaatttt gcttatatgt ttttttgtaa tataatccaa
    14521 tgaactcata ggttcatcta aaagtaatat atcaggttca gctattaagg cacgtgcaaa
    14581 agcaactcgt tgcttttcac ctccacttaa atttttagga tacctatttt ttatatcttc
    14641 tatctgcatt aatttagata agtggttagc cttaagaatc tgttcatcta taggtatttt
    14701 tctcaattta agtggaaacc ttatattatc ttcaacattc atatttggaa ataattcaaa
    14761 attttgatat acaaatccta tatttctatt ttctggaggt aagtttgata ttaaattttc
    14821 tttgtatgat acatttcctt tagcagatgg atatcttcct gctaatgttt ctagaaatag
    14881 agttttacct gacccactag ctcctaataa aacaaagtat tcatgattat taatatttaa
    14941 actattttct tgtaatttaa aattaggtaa atcaagagat aaatttttta tttttaacat
    15001 attaattctt atcaacctct ctattaaaaa atatctcaaa aagcatcaag catgtaagtg
    15061 agataaatat caaaattaca gctattccga ttgcaatgtc tatatcacca gtagacatat
    15121 ttaaatatat agatgtagga ataatttcag ttttcatacg tgtagaacct gctatcatag
    15181 ctactgcacc aaattcacca agtgctcttg accaagtcat tacagttgca gatatgattc
    15241 cagtttttag atttggaaga attataaatc taaatgcttc ccaactatta catccaaggg
    15301 tacgtgcaac aaatatcatc ttttcattac tttcatttag tgaagtgtaa agtattttaa
    15361 tagtaagtgg aagatttaca aatacttgtg ctaaaactat accttgcttt gtaaatataa
    15421 aatcaagctt aaaaatctta taaatggaat ctccaatccc cattcttcca aacaaaagta
    15481 aaagagcaat tcctgatact aagtgtggaa gagacatagg gagatttatt atctgtataa
    15541 tcagtttttt aaatggtaat ttagtaatat gtaaaaaata tgctactgga atagctaata
    15601 aaaggcatac tatagtagat attaaagatg ttttaatact aagtattatt gcatactgta
    15661 cttccactga ttttaagcat ataggaacat aacttatccc tttaatgatt aacataagaa
    15721 gtggtgatac aataaacaaa aagactatag caaaaaatcc agtaagtaga gtagtaaaaa
    15781 atttattttt caagtttaat cactacttaa caggttcaaa accatatttt ttaaatatct
    15841 gtttagcttc gtctgactct atataattta taaatttatt agctaaatct tttttatcac
    15901 tatagtttac gattcctgct gatataagtt ggtctatatt tttatcttgg gcaatttcta
    15961 taacttctac atcttcattg ccaaagaccg aatcttttgt ggctattgta gcatcagctt
    16021 tacttgatgt taactgtaca atcatttcat ttacagttgc agctgtagag acaacattat
    16081 catttataga ttttaaatta tttttttcaa gtattttttg tgtagtctta cctatagcat
    16141 tagactcttt gtctcccaaa ataagtttaa ctccatcttt atttaagtct tccaattttt
    16201 gaatattagc tggatttcct ttaggtacaa ctattgcagg tgtatgatgt gcaaccgttt
    16261 tatgttcacc tacaagttct ttttcaaagg cttttgaata aattggttca gaaccaacaa
    16321 taaatacgtc ccctttgtga cttgtctcaa tttgagctat tagctgtgca gaacctgcat
    16381 agctatattc aactttgaca ttattttctt tctcaaattt ttttgctatt tcatccattg
    16441 gtttctttaa acctgcccct gaatagacaa aaagagtctc tttttctttt gaatcatcag
    16501 ctgttgtcac tttagtatta gattcatcat tagctttgtt ttctttagaa ttacatgcag
    16561 taatacttaa tgaaataatc atagctaaag ctagtactga aagtttttta aacataagat
    16621 attccccctt agtaaattaa tgtaaattct tttttagtat atcatactaa gaaaacgatg
    16681 tctaagtttt tgttatgttt tataatgttt tgttatgttt tgttatgtat agttaagaaa
    16741 aaatctattt ataatgt
//
LOCUS       DAYAPA010000089        16601 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.089, whole genome shotgun sequence.
ACCESSION   DAYAPA010000089 DAYAPA010000000
VERSION     DAYAPA010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16601
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.089"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(584..1318)
                     /locus_tag="ACGI59_002682"
     CDS             complement(584..1318)
                     /locus_tag="ACGI59_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFG9740463.1"
                     /translation="MNLAKGLDVLKISSNVFGEDKVMYIPVIYTEDDATLIDTGLPGQ
                     GDLIIDALNKSNTSFDKLKNIIITHHDIDHIGNINYLREKSKNNIKVYAYKSEISYIT
                     GEETPFKLYMLEQMVDKIDDKMLSMLNVMGLGFKSSYTKVDVSLDNHEKLNLGEEIEV
                     IHTGGHTRGHICLYLKESKVLIAGDLLQVENGELKPVDVMHSNKQELKDAIKNISNYD
                     IETIVFSHGGLYQRNIIGTLKNLIIE"
     gene            complement(2329..2967)
                     /locus_tag="ACGI59_002683"
     CDS             complement(2329..2967)
                     /locus_tag="ACGI59_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HFG9740464.1"
                     /translation="MLIHLVKKDILIVKKYVLLILFIAIGIPLFTLWRLPPFAQILGF
                     VLSIIFSEFMLCQYLLLKESQYSKASVLLCSTPYPRRGLVQSKYVLFAMVFIYCTLVY
                     WIENLLIPQIGTFDLTYILIVLLISSIIYGVYMPIQYKLGYETTKFFFVVIIMASSFV
                     LPLTIAHSNFEIPFIYQYPSMIVNIVLFLLSLIILLASMFISIKIFSNKELV"
     gene            complement(2970..3827)
                     /locus_tag="ACGI59_002684"
     CDS             complement(2970..3827)
                     /locus_tag="ACGI59_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9740465.1"
                     /translation="MENILEVYNLNKTYTDFSLSNISFALPEDCITGFIGVNGAGKTT
                     TIRSILDVVKRDSGKIKIFGKDFDGNEKELKNRIGVVFDDSYFYEELSMAEMKSIIAP
                     SYDNWCEEDYQQNMKRFNLNPKQKIDTLSKGMKMKFALALALSHKAEFLIMDEPTSGL
                     DPLIRSQILDILTGYMKKCGRGVFFSTHITSDLDKIADMIVMIDNGRIILEREKDTLL
                     DEHRKVKGDIQFLNDEIKKLFLNLEITGYGFIGITSKINEVQKNMPDILIERPTIEDI
                     MLAYIERRE"
     gene            complement(3829..4203)
                     /locus_tag="ACGI59_002685"
     CDS             complement(3829..4203)
                     /locus_tag="ACGI59_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFG9740466.1"
                     /translation="MKVLISNTSDKPLYQQIKEQIKEAIFTEELKEGDAMPSIRNFAN
                     DLKVSILTIRRVYEELENEGFLTRQVGIGTFISTGNLEILRDSKRRIVEQKMADMIKD
                     AKALGITQIELQEMMDILYQED"
     gene            complement(5365..6555)
                     /locus_tag="ACGI59_002686"
     CDS             complement(5365..6555)
                     /locus_tag="ACGI59_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFG9740467.1"
                     /translation="MSDMLLWFSVVLVLFAIGDLIASKTKAKVSAVFVTLLLFLILFV
                     TKAIPADIIEKAGMTAAASWSVPMIMFSMGTMLNVKQFIDEWRTVLTAWLGIVAVIVC
                     VSLCIPLFGKSTVLTSIPVINGALPATTIMTQAALEKGLTLAAATATVVFAIQKFIGT
                     PIASRAALQEANRLLVEYHEAKSKGIDLANVDTDKKEAENTGTKVKQAFCEKYDKYYS
                     TNVCIFFIALFSYLGYELSEIIHVNYSIVCLVVGVIVTRIGIVPKDILEKGKIKGFIN
                     MVVFAAVIPSLAKVSLTDLISLFVPIVGMFAASIIGIFLMMKVLPGWKIIGSKPLAFG
                     VGFCQMLGFPTTYLISNEVCNAVGETEEERAYLMSKIMPKLVVGGMACMISIVVAGLM
                     VPML"
     gene            complement(6571..8526)
                     /locus_tag="ACGI59_002687"
     CDS             complement(6571..8526)
                     /locus_tag="ACGI59_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HFG9740468.1"
                     /translation="MEKIKIESSDIFEYIFPHDISCSPDGKHIAYIISNINEEKDCYE
                     HDLYVMDIKTEKQIHMTQTKDVTSFSWISNTELLFTSKRNKPKAGTTDFYTISIEGGE
                     AKKAFSIPKACSVPVSLGNKLWLLTTKNPTDSKKSEPDRAVEGVDYWTFTDKPFIRDG
                     ENFSQRRRVTLELYQEGENITKAITPKFCEVAGIDVSSDKNRILYTGQIYEDCATPFS
                     GLWEYHIDSGETKELVPQGKYQISLAKYIGKDKVMLQASTLDRSITQNHDIFILDLST
                     SEINMIASPDGMYATLLDVDAVYGGGRSNKVIGDKFIGARICRTMTEFNEFDTKTGNI
                     RIITKVDAFTSFDIYDNTMYTVMLKDYELAEIYSIDMTTGTMKKMTAFSKPYLDTHKV
                     SLPEKLTFVAKNKEEVDGFVIPPIDAKEGEKYPAVLFIHGGPKWAYGYMFTHLKQCVT
                     SKGMYVIYCNPHGGDGYGEKFLEMVERWGYVDYEHLMEFVDTCIEKYPGIDADRLGVA
                     GGSYGGYMTNWIIGHTDRFKAAVSQRGISNLITASLIIDFGDRIMKQTCGDKTPWNHE
                     EVLWNHSPIKYVKNVKTPTLFLHSDRDYRCFMGDTFQMFTALKQLGVDTEMYLFHGDT
                     HGLSRNGRPSNRIARANAIVDWFERYL"
     gene            complement(8543..10588)
                     /locus_tag="ACGI59_002688"
     CDS             complement(8543..10588)
                     /locus_tag="ACGI59_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HFG9740469.1"
                     /translation="MKKITKERIENTMKKLFTSETFLEYQFISDCQISPDGGYTAFIV
                     KKADIKENGYTSQVYILNNKTGELKQITSINSVGAYAWEDENTILFPALRNEKVKEAV
                     KNGKQCMSYYALSLNGGEAEELFRLPIKGGKLNPIGKGLYAVIDSYDNDRPIVEGLPE
                     EEQQKKINAYNKRHYEHFKEIPYAVNGEGYISRKRKRLYVYDSNINELKAITAPMFNV
                     VGMKISDGKILYIGQEFKDVKGLKNGVYVFDTNTNTNVCILEKDKYIIKGFELYQNQV
                     ILNLTDALSYGNGENGDFYTIDIDTKEMKLLSGHQHHCIGNTVTSDVKMGAGQTTKVD
                     GEYIYYTSTVDMDCIIERIHIPTGKQEKVTQTGSVDFIDVKDGNIVCVGCLGNGLPEV
                     YTVENGTLCKKTHLNDHILEEYKISVPEYIESKGSSKWEIQGYVYKPVDYEVGKKYPA
                     VLAIHGGPRLTYGPYFMHEIQVFTSAGYFVFFCNPRGSEGRGNAFADIRKQFGEIDYI
                     DFMEFTDTVLEKYTDIDKTKLAVEGGSYGGFMTNWIIGHTNRFAVACAQRSIANWSGM
                     EGTTDIGYYFCKGQTGASHMENHELQWKQSPLAYVDKCVTPTLFLHGEKDYRCYMQEA
                     FQMFSALKIHGCPTKLCLFEGENHELSRSGRPKQKLQRLVEMLDWFSVYIKKDNIGQ"
     gene            complement(10975..11625)
                     /locus_tag="ACGI59_002689"
     CDS             complement(10975..11625)
                     /locus_tag="ACGI59_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFG9740470.1"
                     /translation="MKVKSNLDEILYHKIIESLIRGEYSVGQKILLNDLCEKFEVSRT
                     PVVQAVKMLNKDGVLTIMTNGKVYVPEYEYDMVKQVCETRTLIETYALEKMMQEEEEV
                     FQQKLDVIKRYSDKCEEYYRQEKSVELALADLELHKAIVEGANNEILNDVYVGIQGRF
                     IVVNYLIRPLKNRNYEGTVQDHFEILKFIEDRDTKKAVDKLRNHIQGTIKRFCEDE"
     gene            complement(12609..12782)
                     /locus_tag="ACGI59_002690"
     CDS             complement(12609..12782)
                     /locus_tag="ACGI59_002690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9740471.1"
                     /translation="MLKQMLENNLPIKIVSWDSEELYRNSLNSCSLGNYVPLIEYLSS
                     LEDFREVYKMLWK"
     gene            complement(12786..12941)
                     /locus_tag="ACGI59_002691"
                     /pseudo
     CDS             complement(12786..12941)
                     /locus_tag="ACGI59_002691"
                     /inference="COORDINATES: protein motif:HMM:NF014691.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
     gene            complement(13042..13722)
                     /locus_tag="ACGI59_002692"
     CDS             complement(13042..13722)
                     /locus_tag="ACGI59_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFG9740472.1"
                     /translation="MIHLMKLELKKVGLKRYFLFSMLGIFLCMFFVFTGLNDKSTSIY
                     DYDVTFRTISLFFCFYYIILFAVLNVAYIINEYTNKTILILFSYPLDRKKLILSKLLL
                     ITTLMISSMIVGYICCFTFVVVLDKYFNLVIGDFHISILSYWIPGAIKSIIVFCSLGI
                     WTFVVGMIRKSIPVTLVSSIIFINLRQFILAGTNTTQDSLISVFIIVGITVIGIYYIL
                     NHKVTEID"
     gene            complement(13719..14645)
                     /locus_tag="ACGI59_002693"
     CDS             complement(13719..14645)
                     /locus_tag="ACGI59_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9740473.1"
                     /translation="MNYIIETNQLKKIYKDKAVVNAVNIHVKKGEIYGFVGPNGAGKS
                     TVMKMLLNLVKPNSGEIVMFGKKVAETDFEILKKIGTIIENPYLYENLTAKQNLDLHC
                     EYMGYYNKEHISEVLECVGLSKQSNKKVSKYSLGMKQRLAIARAILTKPELLILDEPI
                     NALDPEGIREMRELFRKLNTDYGITIFISSHILSEVEQIADTIGIIQDGKLIKEISMS
                     DIHKYQTDYVEVDVDNVELAGYLLEKEFGIKDFKITSESCIEIYDLRKDVKEISKIFI
                     QNQIGINSIGRKQSSLEDYFFQTTGTGGKETL"
     gene            complement(14718..15635)
                     /locus_tag="ACGI59_002694"
     CDS             complement(14718..15635)
                     /locus_tag="ACGI59_002694"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFG9740474.1"
                     /translation="MELFLVVISIVLFALYLKERNKSKKMESDYKYINSRLRDITNDD
                     EINYILVPSDISIVKETAVDINKLLEKFYSRQINYNRSEKAMLQIFTNISHDLRTPIT
                     VLKGYIEMLYLQSQKEELSSSMRATVEKMQTNSNELVNSINNLFNMAKIQSGDMILNL
                     QKSNLTQLCHEIILEFYDLLDKENFNVEVNIEDRPIYANVDTEALQRILKNLIDNAIK
                     YGSDGRFLGISLYEKEKHVFIDIEDKGSGISETEKEHIFTRAYTADRKKGNGLGLAIS
                     QGLVNSMGGSIYVNSRPNVKTVFTVKLKS"
     gene            complement(15635..16324)
                     /locus_tag="ACGI59_002695"
     CDS             complement(15635..16324)
                     /locus_tag="ACGI59_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFG9740475.1"
                     /translation="MDILLVEDDKNIREMVYDFLISENYSVKTAIDGNTAIEIFKNYS
                     FDIILLDLMLPKSSGFEVLKYIRSVSVVPVIITTAKDSDTDKMLGLNLGADDYITKPF
                     SIVELLARIKANIRRATVYSSSRNSEDMVIQYKDLIINISKYVVSKNGIDLNLTHTEF
                     EIVKLLATNQGRAFSKEQLYNAIWKEPYYGNENVLNTHINRLRNKLSDGIEKNNYIKT
                     LWGIGYKMEEE"
BASE COUNT         5673 a         2805 c         1830 g         6293 t
ORIGIN      
        1 atggtttaaa taatcttctt tactttgtat tatgatacat gctttaaata tttttacgca
       61 ctttatatta taatacatgc tttaaataat cttattcgct ttgtattata acacattatt
      121 taaatatttt tactcactct gcattatagt acattattta aatatttttc ttcgctttgc
      181 attatgatac attatttaaa tatttttact caccttgtat tatggtacat tatctaaata
      241 tttttcttca ctctgtatta tgatatataa cttaaatatt ttacctcact ctacaatatt
      301 atatataatt taaatatttt tcttcacttt gtatactaaa gtttatttaa agtattttct
      361 ttcccattat actatgctgt taaatatttt tcaactgaag tattgtagaa ccttaaaatt
      421 actataaaaa actatattct aaaatacttg taatactacg tatcaccctt aacacttaag
      481 tataatttta aagcaaaatt cttattactt atatttaatt ttgattctac atctttagat
      541 ataattttaa gtaatttaag aaattattgt aaatcatata gtactactca ataattaggt
      601 ttttcaatgt tccaattatg tttctttgat ataatccacc atgactaaaa actatcgttt
      661 ctatatcata attacttata tttttaatag catcttttaa ctcttgtttg ttgctgtgca
      721 ttacatcaac aggcttaagt tctccatttt ctacctgtaa caaatctcca gcaataagta
      781 ctttagactc ttttaaataa agacatatgt gacctctagt gtgacctcct gtatgtataa
      841 cttctatttc ctcacctaga ttcaattttt catggttatc taatgacaca tctactttag
      901 tatatgatga tttaaatcca agtcccataa catttagcat actaagcatc ttatcatcaa
      961 ttttatctac catttgttcc aacatataaa gtttaaaagg agtttcttct cctgttatat
     1021 agctgatttc acttttgtat gcatatactt tgatattatt cttagatttt tctcttagat
     1081 aatttatgtt tcctatatgg tctatatcat gatgagtaat tattatattt tttaacttat
     1141 caaagctcgt gttactttta ttaagagcat caattattaa atcaccttga cccggaagac
     1201 ctgtgtcaat aagtgttgca tcatcttctg tgtagattac aggaatatac ataactttat
     1261 cttcaccgaa tacatttgat gaaattttta aaacatctaa tccctttgct aaattcataa
     1321 tttttctcct tataattact taattagagg tcaatttaat taaagaaatt gagaaaatgt
     1381 agattttcct ctagttatat tttattaatt aaaaattatt aatacctagt atatcactta
     1441 agttgaagga ttactcgact tgattttttt gttattttat ggtatagtaa ttaatataga
     1501 agtaagtaat tgcaagcttt gcaattactt ttattttttt ctaataacta ttttattttt
     1561 gataattcgt ataaaattaa aagtaaagta atatggaaaa tatgaatata gaaaaacttt
     1621 aataagaaaa taaattatga ataaataaaa gaaacgcaat actgtatata gatgattatt
     1681 tagaaaaata aaatctcttt taataaaaat aagcttcaaa ataaactttt agttcataaa
     1741 gcttaggagg aactaaattg agtgcaagtg tatctactgt tcaaaataaa tagttcataa
     1801 agcactcttt tttatattaa atcaattata ttttcgtcat ttcagcaatg aaaaagaaac
     1861 ttatctctat tttgagacag cttcttttta ttaacttcct actttttatt agttttctaa
     1921 aattgaaaag ctttatttat aggattcctt agatttatat tttgacgata taatttttta
     1981 aaaacatctt aaaagatggt atacaataca cacaatcata ctcataattg aaaaataatc
     2041 aatggaaaat aaaattactg gatattatct attaaaaaat acaaattgat tcacaagaaa
     2101 catataatct ataatattat gatgttactc tctcgcacca agtaacttta ttttcaagta
     2161 accttttaaa ttcataccat ctaacttcac tcaataaaaa tggcaatata aatatgtact
     2221 ctatttttta cttttaaata tctgacaagc tttatatact attggaagta aaatacaaag
     2281 taaaacagcc caatctggta aatgtggcat ataaaagtcc cccttacctt atacaagctc
     2341 cttattacta aaaattttaa tagaaataaa cattgaagct aacagtataa ttaaacttaa
     2401 aaggaacaat acaatattca ctatcatgga tggatactga tatataaatg gaatttcaaa
     2461 attgctgtga gctatagtta atggtaatac aaatgatgaa gccatgatta tcaccacgaa
     2521 aaagaatttt gttgtttcat atcctaattt atattgaata ggcatataaa ctccataaat
     2581 aatagaactt attaataaaa caatcaaaat atatgttaag tcaaaagttc ctatttgtgg
     2641 aataagtaaa ttttcaatcc agtaaactaa tgtacaatag ataaatacca ttgcaaataa
     2701 aacatactta gattgaacta agccacgtct aggataagga gttgaacaaa gtaatacaga
     2761 tgcttttgaa tattgactct cctttaataa tagatactgg cacagcataa attctgaaaa
     2821 tataatggat agtacaaatc ccaatatttg tgcaaatgga ggtaaacgcc acaatgtaaa
     2881 taatgggatt cctattgcaa taaacaatat caacaataca tattttttta caatcagaat
     2941 atctttttta actaaatgaa tcaacataat tactctctcc tttcaatata tgcaagcata
     3001 atatcttcaa ttgtaggacg ctctattaaa atatctggca tatttttttg cacttcatta
     3061 atttttgagg taatccctat aaaaccataa cctgtaattt ctaaatttaa aaataatttt
     3121 ttgatttcat catttagaaa ttgaatatcc ccttttactt ttctatgttc gtccaataga
     3181 gtatcttttt cacgctctaa tataatacgt ccattgtcaa tcataacaat catatctgct
     3241 attttgtcta aatcagatgt tatatgagtg gaaaagaaaa caccacgacc acattttttc
     3301 atatatcctg ttagtatgtc aagtatctga ctacggatta gtgggtctaa accacttgta
     3361 ggttcatcca taattaaaaa ctcagcttta tgcgataatg ctaatgctaa tgcaaatttc
     3421 attttcattc cttttgaaag tgtatcaatc ttttgttttg ggttaagatt aaaacgcttc
     3481 atattttgtt gataatcttc ttcacaccaa ttatcatatg aaggtgcaat aatacttttc
     3541 atttctgcca ttgacaactc ctcatagaaa tatgaatcat caaatacaac tccaattcga
     3601 ttttttaatt ccttctcatt tccatcgaaa tctttaccaa agatttttat ttttccacta
     3661 tcacgcttta cgacatccaa tatcgaacgg attgttgttg tttttcctgc tccattaaca
     3721 cctataaatc cagttataca atcttctggt aatgcaaagc ttatattaga taatgaaaaa
     3781 tcggtataag tcttatttaa gttataaact tctaaaatat tttccatatt aatcctcctg
     3841 atatagaata tccatcattt cttgtaactc tatctgtgta attcctagtg cttttgcatc
     3901 cttaatcata tctgccattt tttgttcaac tatacgtctc tttgagtctc ttagtatttc
     3961 taaatttcca gttgaaataa atgttcctat tccaacttgg cgtgtaagaa agccttcatt
     4021 ttcaagttct tcatacactc gtctaatagt caaaatacta acttttaaat cattagcaaa
     4081 atttcttatg gatggcattg catccccttc tttcagttct tcagtaaata ttgcttcttt
     4141 aatttgctct ttaatttgtt ggtataatgg cttatctgat gtgttagaaa tgagcacttt
     4201 cattttttat cacccccttt ttatcatttt cagtgtgatg ttgttatata cacactatac
     4261 acttagagca cttttttgtc aatataaaag atgtaaaata ttactatgac aaaataaata
     4321 tgaatgaaat attagagatt gttgatagct ataaacactt tttaaaacca cataagatag
     4381 aatgtattaa atatttatta aaagtaagaa ttgaatatct aaaaaaaaga ggaatcctgt
     4441 atgaatgatt tgtttgatga taaattaagt tataaactat ttataaaaaa cataaaggaa
     4501 taaatccatt tctaattaca aacatactaa aaaatttaga tagtaataaa tagatagtaa
     4561 taaatagata gtaataaatg ggatgaattt ataagaattt ggatagagga aagagttatt
     4621 caagagtgta gactagaggt aaactctata ttaaaaaaat ttaaatttgg atattatgat
     4681 gcatggaata tttgcagaag taaccttgct ataagtatag aagatttttt tgattacaaa
     4741 agataatact ctaaaatgtg aggatttcaa cattcaatac tgtactttaa ataatatcac
     4801 tgcaaaatac catccacctt ttaccataat tgaataccct aattatttta aaaaagaaaa
     4861 cattagaaac agaaaacctt ggatatataa aataaaaaat actaacttct aaaacacgga
     4921 tactctttga taaataaaac aaaaaataat tttaataatt aaaattggct atctcaaact
     4981 agagataagt ctctttctca cttttaaaac gttgaaaata tattcgattt cgtacaaaaa
     5041 agagtgcctt agaactaaaa agttcacttt gtggcactcc cttttttctc ctctttttaa
     5101 ttttttataa aaattcttca aaaatattat taaataccct atattataca taaatcaaat
     5161 taaagttgca ttttagcaaa taaaaacaaa ttataactat tagcatagca aataaaaaat
     5221 ctgctctctt ataaaatcaa attataatta taaaaagaaa atattcttat aacaaattag
     5281 ctaatataaa attattttat aaattaatac tactttttag cttctcctat aaattaatac
     5341 cactttttta actttgaaac ctatctacaa cattggtacc ataagaccag ctaccacaat
     5401 agaaatcata caagccatac caccaactac cagcttaggc ataatcttag acatcaaata
     5461 tgctctttct tcttccgttt ctccaacagc attacacact tcatttgaaa ttaaatatgt
     5521 agttgggaat cccaacatct gacaaaaccc tactccaaaa gcaagaggtt tagaaccgat
     5581 aattttccaa cctggtaaca ccttcatcat aaggaatata ccaatgatag aagctgcaaa
     5641 catacccaca attggtacaa aaagactaat taaatctgtc aaagaaactt ttgctaaaga
     5701 aggaataaca gctgcaaaca caaccatgtt gataaatccc ttgatttttc ctttttccaa
     5761 aatgtctttt ggcacaatac caatacgtgt tacaataaca ccaacaacaa gacatacaat
     5821 agaataattt acatgaataa tctctgaaag ttcataccct agataagaga ataatgctat
     5881 aaaaaatata caaacatttg tagaataata tttatcgtat ttttcacaga aagcttgttt
     5941 tacttttgtg cctgtattct ctgcttcttt cttatcagta tcaacatttg ctaaatcaat
     6001 gccttttgat tttgcttcat gatattctac taaaagacgg tttgcctctt gtaacgctgc
     6061 tcttgaagct attggtgtac caataaactt ctgaattgca aatacaactg ttgctgtagc
     6121 tgctgcaagt gtcagtcctt tttctagtgc tgcttgtgtc ataatagtag tagcaggtaa
     6181 agctccattt ataactggta tgcttgtcaa tactgttgat tttccaaaca aaggaataca
     6241 taatgagaca cagactataa ctgctacaat tccaagccat gctgtcaata ctgttctcca
     6301 ttcgtctata aattgtttaa cattcaacat tgttcccata ctaaacataa tcattggaac
     6361 actccaacta gctgctgcgg tcatacctgc tttttcaata atatcagctg gtattgcttt
     6421 tgtgacaaat agaatcaaaa ataaaagtag tgtaacaaat acagctgata cttttgcttt
     6481 tgttttgctt gcaattaaat ccccaatagc aaataaaact aatacgacac taaaccataa
     6541 taacatatca ctcatagaat aaccccctta ttataaatat ctttcaaacc aatctacaat
     6601 agcatttgct cttgcaattc tattagatgg tcttccattt ctagacaatc catgtgtatc
     6661 tccgtggaac aaatacattt ctgtatctac accaagctgt tttaatgctg taaacatttg
     6721 gaaagtatcc cccataaaac atctataatc cctatcacta tgtaagaaca atgttggtgt
     6781 cttaacattt ttcacatatt taataggtga atgattccaa agaacctcct catgattcca
     6841 aggcgtctta tctccacaag tctgcttcat aattctatcc ccaaagtcaa taattaaaga
     6901 agctgtaatc aagttactaa tacctctttg agaaactgct gctttgaaac ggtctgtatg
     6961 accaattatc cagtttgtca tataaccacc gtaacttccc cctgcaaccc ctaagcggtc
     7021 tgcatcaatc ccaggatatt tttcaataca agtatctaca aattccataa gatgctcata
     7081 gtcaacatac ccccatcttt ctaccatttc taagaatttt tcaccataac cgtcaccacc
     7141 atgagggtta caataaatca catacattcc ctttgatgtt acacactgct tcaagtgtgt
     7201 aaacatatag ccatatgccc atttaggccc accatgaatg aacaatacag caggatattt
     7261 ttcgccttct ttagcatcta taggtggtat aacaaaacca tcaacttctt ctttattttt
     7321 tgctacaaac gtcaattttt caggtaatga aaccttatga gtatctaaat atggtttaga
     7381 aaaagcagtc atttttttca ttgtacctgt agtcatatca atgctatata tttctgctaa
     7441 ctcataatct ttcaacatta ctgtgtacat ggtgttgtcg taaatatcaa aagatgtaaa
     7501 agcatctact tttgtgatga tacgtatatt acctgtcttt gtatcaaact cattaaattc
     7561 tgtcatagtt ctgcaaattc ttgcaccaat aaatttatct cctataactt tattactacg
     7621 tccaccacca tatactgcat ctacatccag taatgtagca tacataccat ctggactagc
     7681 aatcatattt atttcactag tactcaaatc aagtataaaa atatcatgat tctgtgtaat
     7741 agaacggtct aatgttgaag cttgcaacat tactttatct ttgccaatat attttgccaa
     7801 acttatttgg tactttcctt gtggtacaag ttctttggtt tcaccactgt ctatatgata
     7861 ctcccaaagt ccagaaaaag gagttgcaca atcttcataa atctgaccag tatataaaat
     7921 tctgttttta tccgaagaaa catctatacc tgctacttca caaaattttg gtgtaatggc
     7981 ttttgttata ttttcacctt cttgatataa ttctaatgtt acacgtcttc tttgagaaaa
     8041 attttctcca tctcttataa aaggtttatc tgtaaatgtc caataatcta caccctctac
     8101 tgccctatct ggttctgatt ttttagaatc cgttggattt tttgttgtca gtaaccacaa
     8161 tttatttcca agtgatacag gtacgctaca agcttttgga atagaaaatg ctttttttgc
     8221 ttctcctcct tctatagaaa tagtgtaaaa atctgttgta cctgcttttg gcttatttct
     8281 tttagatgta aaaagtagtt ctgtattaga aatccaacta aatgatgtta catcttttgt
     8341 ttgagtcata tgtatttgtt tttctgtttt gatgtccatc acatataagt catgttcata
     8401 gcagtctttt tcctcgttga tatttgatat aatataagca atatgctttc catcaggtga
     8461 acaagaaata tcatgaggga atatatattc aaaaatatct gaactttcaa tctttatttt
     8521 ttccattttt atattctcct ttctactgtc ctatgttatc tttttttata tataccgaaa
     8581 accaatctag catttcaact aatctttgta gcttttgttt tggtctacca gaacgagaca
     8641 gttcatggtt ttctccttca aatagacaaa gttttgtagg acacccatga attttcaatg
     8701 ctgaaaacat ctggaatgct tcttgcatat agcatctata gtctttttca ccatgtaaaa
     8761 ataaagtcgg tgtaacacat ttatcaacat aagcaagagg tgactgtttc cattgaagct
     8821 cgtggttttc catatgagaa gcacctgttt gtcctttgca gaaataataa ccaatgtctg
     8881 ttgtaccctc catgccactc cagtttgcaa tagaacgctg agcacaagca acagcaaaac
     8941 ggtttgtatg accaattatc caattcgtca taaatccacc ataacttcca ccttcaacag
     9001 ccaattttgt tttatcaatg tctgtatatt tttcaagtac agtatctgta aattccataa
     9061 aatctatata atcaatttct ccaaactgtt tcctaatatc tgcaaaagca ttgccacgac
     9121 cttcagaccc ccttgggtta cagaagaata caaagtaacc tgcacttgta aatacctgta
     9181 tctcatgcat aaaatatggt ccatatgtca atcttggtcc tccatgaatc gccaatacag
     9241 ctggatattt tttccccacc tcataatcta ctggtttata tacgtatcct tgtatttccc
     9301 atttagaact tccttttgac tctatgtatt ctggcacaga aattttatat tcctctaaaa
     9361 tatggtcatt taaatgtgtt ttcttacaca atgttccatt ctctactgta taaacttctg
     9421 gtaatccatt tcctaaacaa cccacacata caatattgcc atcttttaca tctataaaat
     9481 caactgaacc tgtctgtgtt actttttcct gttttcctgt tggaatatgt attctttcaa
     9541 taatgcagtc catatctact gtagaagtgt aataaatata ttctccatct acctttgttg
     9601 tctgaccagc acccatttta acatcagatg ttactgtatt tccaatacaa tgatgttgat
     9661 gccctgaaag aagtttcatt tctttcgtat caatatcaat agtataaaaa tctccatttt
     9721 ctccatttcc ataagataaa gcatcagtta ggtttagtat cacttgattt tgatataatt
     9781 caaaaccttt tataatatac ttatcttttt ctaatataca gacatttgtg tttgtgtttg
     9841 tatcaaatac atagacccca tttttcaaac cttttacatc tttaaattct tgccctatat
     9901 ataagatttt tccatcactt attttcatac ctacaacatt aaacataggt gctgtaattg
     9961 ccttcaactc atttatatta ctatcgtata catataggcg ttttcttttt ctgctaatat
    10021 atccctcccc attgacagca taaggaattt ctttgaaatg ttcataatgt cttttgttat
    10081 aagcatttat ttttttctgt tgttcttctt ctggcaatcc ctctacaata ggtcggtcat
    10141 tgtcataaga atcaataact gcatacaaac cttttcctat aggattcaat ttaccacctt
    10201 tgatagggag tctaaaaagt tcttctgctt ctccaccatt taaagataaa gcatagtaac
    10261 tcatgcattg cttaccgttt ttgacagctt cttttacttt ttcatttcgc aaagcaggga
    10321 acaagatagt attttcatct tcccaggcat atgctccaac agagtttata gacgttattt
    10381 gttttaattc gccagtttta ttgtttaata tataaacttg gcttgtgtaa ccattctctt
    10441 tgatatcagc tttttttaca ataaatgctg tatatcctcc atctggtgat atttggcaat
    10501 cacttataaa ttgatattcc aaaaatgttt cactcgtaaa tagctttttc atagtatttt
    10561 ctatcctttc tttagtaatt tttttcaaac tccctaccac ctctttcatt ttttatttta
    10621 aatttaaaat aaaaaatttt aaatttaaaa taaaaaattt tataaaggtt atttttttag
    10681 gaagccttct ggcttccttg taatatttgt aatttaaagt atatttaatc catgcttaat
    10741 tcatgcttaa ttcatactta atctatactt aatctatact tagtccatac ttaatctata
    10801 tttaattcat acttagactt atttacacta tatttagatt atatttatta gattatattt
    10861 attagattat atttattaga ttatatttag attatgttta aactataatt agattatact
    10921 tagactacat ttagcaactt aatagataag tatatatagt aacttcaaaa cttattattc
    10981 atcttcacag aatcttttaa tagttccttg aatatgattt cttagtttat ctactgcttt
    11041 ttttgtatct ctgtcttcaa tgaattttaa tatttcaaaa tggtcttgta ctgttccttc
    11101 ataattacga ttttttaatg gtcgaattaa atagttcact actataaatc ttccttgtat
    11161 accaacatat acatcattta aaatctcatt atttgcccct tctacaatgg ctttatgtaa
    11221 ttccaaatct gctagtgcaa gttctacaga tttttcttga cgatagtact cctcgcattt
    11281 atcagagtat ctttttatta catctagctt ttgttgaaaa acctcttcct cttcttgcat
    11341 cattttttct aaagcatatg tttcaatcag tgttctcgtt tcacaaactt gttttaccat
    11401 atcatattca tactctggta cgtacacttt accatttgtc ataatagtaa gcactccatc
    11461 tttatttagc atttttactg cttgtacaac tggtgttctg cttacttcaa atttttcaca
    11521 aaggtcattt agtaatattt tttgacctac agagtattca ccacgaataa gactttcaat
    11581 aatcttatga tataaaattt catctaaatt tgactttact ttcactactc tcaccttatt
    11641 tctattcttt tgccatatta tattgaatta ctggacttta attactttta gtaatatttt
    11701 taaagtagaa attcttattt gatagtactt ttgtactaca atttaaatta ttgtactgta
    11761 aatcatgctc ttatactata gattaattca ttgtattgca aatcaagtta ttgtactata
    11821 gattaaatcg ttatacttca aattaaacta ttatactata gattaaacca ttgtacttca
    11881 aattaaacta ttatactata gattaaatcg ttgtacttca aattaaacta ttatactata
    11941 gattaattca ttgtacttca aattaaacta ttataccata gattaattca ttgtattgca
    12001 aatcaagtta ttgtactata gattaaatcg ttgtacttca aatcaagtta ttgtactata
    12061 gattaaatcg ttgtacttta aattaaacta ttatactata gattaattca ttgttctgca
    12121 aatcatgcta gtgtactaca aattaaacta taaatgttta ctatctctta ataatttgaa
    12181 taatatgtta caaaaagtat agcacctttt tatttttttc gcaataaata aaaaatttaa
    12241 aattttttat tttttatttt cttatattat caatttgaaa tatgaaagat taagactaat
    12301 agaattgatt cagttaattg aaaaaaatag gtacctttta tgaaaattct aatgataagc
    12361 atttttctga tgtaatggta aattataaat gataaaatta aaaatcactt ttagttttat
    12421 gaactgtccc catattaaga aacaactaag atacataaaa atagttataa agagatagtt
    12481 aaactatatc cactatttta tatagttaaa atagtgggta tagtccaagc taagaataac
    12541 taagaaatta gcgttaaata gcatagataa aaatacattt atatataaac tttttttgct
    12601 gcaaacaatt atttccataa cattttatat acttctctaa aatcttctaa acttgataaa
    12661 tattcaatta aagggacata attacctaaa ctacaagaat ttaaggagtt tctatacaat
    12721 tcctcactat cccaagatac aatttttata ggaagattat tttctaacat ctgttttagc
    12781 ataacaaatc tacctatgtg accattacca tcttgaaatg gatgtatcaa ttcaaatcta
    12841 taatgaaatt cagttataac tttaatagat acttcactct gactatagta ccattcaatt
    12901 aattcatcaa gctttggctc aacttcaaat ggttgtgcaa tattattaaa actctaataa
    12961 tattaataat agctgatata atagcaaatc attattatcc cgagaagata caattaataa
    13021 ctaccttcct atccacagta tctagtcaat ttcagtaact ttatgattta gtatataata
    13081 tataccaatc acagttatac caactataat aaaaacagaa atcaaactgt cttgagttgt
    13141 atttgtacca gctaaaataa attgtcttaa attgataaaa ataattgaac tgacaagggt
    13201 aacaggtatt gattttctta tcattccaac aacgaatgtc catataccta atgagcaaaa
    13261 tacaataata cttttaattg ctcctggtat ccaataagat aaaattgaaa tatgaaaatc
    13321 tccaataact aaattaaaat acttatccaa cacaacaacg aatgtaaaac aacagatata
    13381 tccaactatc attgatgata tcataagagt cgtaatcaaa agtaactttg ataaaataag
    13441 ttttttacgg tcaagtggat aagagaataa aatcaagatt gttttatttg tatattcatt
    13501 tattatatat gccacattca acacagcaaa taatataata tagtaaaaac agaaaaataa
    13561 actgattgtc ctaaatgtaa catcataatc atatattgaa gtagatttat cattcaatcc
    13621 agtaaagaca aaaaacatac ataagaaaat ccccagcatt gaaaataaaa aatatctttt
    13681 tagcccaact ttttttagct ctagtttcat taaatgtatc atagtgtttc tttacctcct
    13741 gtacctgtag tttggaagaa gtaatcttct aaagaacttt gtttacgtcc aatgctattt
    13801 ataccaattt gattttgaat aaatattttg gatatttctt ttacatcttt tcgtaaatca
    13861 tatatttcaa tacaactttc cgaagtaatt ttaaagtctt ttattccaaa ttctttttct
    13921 aacaagtatc cagcaagctc cacattatct acatctactt caacataatc tgtttgatat
    13981 ttgtgtatat ctgacataga aatttcttta ataagtttac catcttgaat aattccaata
    14041 gtgtccgcaa tttgttctac ctctgaaaga atatgactag aaataaatat agttattcca
    14101 taatctgtat ttaacttacg gaataattct cgcatttctc ttatcccctc tgggtcgagt
    14161 gcatttatag gttcatcaag aatcaataat tctggtttag taagaattgc tctagctatt
    14221 gcaagacgtt gtttcatccc taaagaatat tttgaaactt tcttgtttga ttgctttgac
    14281 aatccgacac attccaatac ctctgatata tgttctttgt tataataacc catatattca
    14341 cagtgtaaat caagattctg tttagcagtc aaattctcat ataaatacgg attttcaata
    14401 atagtaccaa tcttttttaa tatttcaaaa tcagtttctg ccactttctt accgaacata
    14461 actatttctc cagaatttgg cttaacaaga tttagcaaca ttttcattac tgtactttta
    14521 cctgccccgt ttggtccaac aaatccataa atttctccct ttttcacatg aatattaaca
    14581 gcattaacaa cagctttatc tttatatatt ttctttaatt gattggtctc tatgatatag
    14641 ttcatcatac tccctccttt ctaaaaacag tatacaaaat aaaattatct tttctctctt
    14701 ttaaatctaa cttttttcta acttttcaat tttactgtaa aaactgtttt tacattaggt
    14761 ctactattta cataaattga acctcccata gagtttacaa gcccttgaga aatagccaaa
    14821 cctaaaccat tgcctttttt tctgtcagca gtataagctc ttgtgaaaat atgctctttt
    14881 tcagtttcag aaatacctga acctttatcc tcaatatcta taaaaacatg tttttccttt
    14941 tcatatagtg aaatccctaa aaatctacca tctgaaccat attttatagc attatcaatt
    15001 aaatttttga gtatacgttg taatgcttct gtgtcaacat ttgcatatat aggtctatct
    15061 tcaatattaa cttcaacatt aaagttttct ttatctagca aatcataaaa ctctaaaata
    15121 atctcatgac ataattgtgt tagattggat ttttgaagat ttaatatcat atctccagat
    15181 tggatttttg ccatattaaa caggttattt attgaattaa caagctcatt tgaatttgtt
    15241 tgcatttttt ccacagttgc tctcattgaa gaagataatt cttctttttg actttgaaga
    15301 tataacattt caatataacc ctttaatact gtgatgggag ttcgtaaatc gtgtgatatg
    15361 tttgtaaata tttgcaacat cgctttttca cttctattat aattaatttg cctgctgtaa
    15421 aacttttcaa gaagtttatt tatatctact gctgtttctt ttacaatcga aatatcagat
    15481 ggtactaaaa tataattaat ttcatcatca tttgttatgt cacgaagtct tgaattgata
    15541 tacttataat cactttccat tttctttgat ttatttcgtt cttttaagta gagagcaaat
    15601 agtacaatcg aaattacaac taaaaataac tccattattc ttcctccatt ttgtagccaa
    15661 ttccccaaag agttttaata taattatttt tttctatacc atcagacaat ttatttctta
    15721 acctgttaat atgagtattt agcacatttt catttccata ataaggttct ttccaaatag
    15781 cgttgtataa ttgttccttt gaaaaagcac gtccttgatt agtcgctagt aatttaacaa
    15841 tttcaaattc tgtatgtgtt aagtttaagt caattccatt ctttgaaact acatattttg
    15901 atatatttat aatcaaatct ttatactgaa taaccatatc ttcagagttt cttgaactgc
    15961 tgtacactgt tgcacgtcta atattagctt taattcttgc aagcaattct acgattgaaa
    16021 aaggctttgt aatataatca tctgcaccaa gattaagccc tagcatttta tctgtgtcac
    16081 tatctttcgc agtagtaata ataacaggaa caacactcac acttcgtata tattttaata
    16141 cttcaaaacc actagacttt ggtaacatca agtccagtaa aataatatca aatgaataat
    16201 ttttaaatat ttcaatagct gtatttccat caatggcagt tttaacagag taattctcac
    16261 taatcagaaa atcgtatacc atttctcgaa tattcttatc atcttcaacc aataagatgt
    16321 ccattattat tcaactcctt ttcgtcagta tacttcaaat gatatattta ttaaaaaggg
    16381 ttgtcaataa ataactgctt atgatattgt atctgcaatt tggtgatact cttctaagtt
    16441 aaagttcttt tcaataagct ttattgaaaa aataattaaa atatagtata ctctcttatc
    16501 tttatctgaa atatacataa ttttctttta ttaacatcta caattaacta ttttatatta
    16561 taaaacacct tttaactctt gctaaaaaat attttataca t
//
LOCUS       DAYAPA010000090        16567 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.090, whole genome shotgun sequence.
ACCESSION   DAYAPA010000090 DAYAPA010000000
VERSION     DAYAPA010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16567
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.090"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(188..1717)
                     /locus_tag="ACGI59_002696"
     CDS             complement(188..1717)
                     /locus_tag="ACGI59_002696"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase domain-containing protein"
                     /protein_id="HFG9740476.1"
                     /translation="MKSLKALIVGVSKYSIVKNKDLLFCKNDIIEINNVLHKRLGITS
                     NNIRTLGMEYEVRKDLFFKTFQEISDSIGDDDTFIFYFSGHGCICNNGVHYLVLSDEL
                     VSIQFVIDFIEGLNCKNKIVFIDCCFAGKFNVSDKQKINIKDIFSLFDRKGCAVLVSS
                     SEYQESFSLPDSSISRFTSLLSEALRNEYIVRKGELSLRAISKYINLRMSILNKNYIN
                     CKQNPIFRSNILGDIYFNVKDYKSYIKKKVFQEHDDYIIYNVKSLHISIIKRYSVEVI
                     LKNKFSYCDISEISKEVFDIVKSVEVYNSEIGEDRLKGKDANIIWVYFGKDEIDMING
                     IYMCKTTWIDKYQEKDIWYSVNGKNKFFINGVHTETYDNYESLKSYISQNTITKEKAV
                     YEVKEILTNMINISEEIISFYNEYENKKICEDNIFQIIDSRKKKMDECYNKVINLDFP
                     PIEIKDWFDKCINLVSTIQNFGVYWDHSRTEENRKQCMEMNIEDYYKDLREVIRLSEE
                     I"
     gene            complement(2416..3846)
                     /gene="dcm"
                     /locus_tag="ACGI59_002697"
     CDS             complement(2416..3846)
                     /gene="dcm"
                     /locus_tag="ACGI59_002697"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF012372.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HFG9740477.1"
                     /translation="MKKITIDKNRVRKIMKEKNIRSQNQLALNMGITKNQLSAILSEK
                     FTPIKSNVEKLCEVLDIGFDEIMCLEDVNEKQLKFTNICSSNKDYIEHRDCTYKELSE
                     YELNPRERNKNNKHIDISNVLAKRAYTCVELFAGAGGLALGLEKAGFKEVGLVEWDKY
                     ACDTLKLNRPDWNVIQGDIVKIAENGIKNYIDNNIEIDLLSGGYPCQAFSYAGKKLGL
                     EDVRGTLFYSYAKILEELKPKVFLAENVRGLVSHDGGKTLKTMIDVFSEIGYNVKYEI
                     LKAVNYGVAQKRERVIIIGTRKDLSHVDFKFPKSFEYVATLRDALKNVPKSEGARYPE
                     RKKKVLDMVPPGGCWIDLPDEVARDYMGKSYYSGGGKRGMARRISWDEPCLTLTCSPA
                     QKQTERCHPDETRPFTTREYARIQSFPDEWEFTGSVSQVYKQIGNAVPVKLGKAIGLS
                     IVDYLNKIEKIYVTEEVAITEEEYVI"
     gene            4059..4775
                     /locus_tag="ACGI59_002698"
     CDS             4059..4775
                     /locus_tag="ACGI59_002698"
                     /inference="COORDINATES: protein motif:HMM:NF025864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PmeII family type II restriction endonuclease"
                     /protein_id="HFG9740478.1"
                     /translation="MTELEKQEILDKAKDFFTNILVANHKKNTIKLNLKAFNINPFLV
                     KYLASFLTGSNKPEDIAKALIYPRVLGTSINTSFGTNLQKFCITTLDGFASTTSGIDI
                     EFIDCIDGRRKFCQIKAGPNTINKDDVKTIIDHFSSVKRLARTNGLSLSYNDLIVGVF
                     YGTENELNAHYKRINEEYPVVVGQDFWYRLTGDENFYFDLIDTIAQSALEEDCTVLLK
                     KTISNLADEISKDKSLFNQK"
     gene            complement(5020..5298)
                     /locus_tag="ACGI59_002699"
     CDS             complement(5020..5298)
                     /locus_tag="ACGI59_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6809 family protein"
                     /protein_id="HFG9740479.1"
                     /translation="MYDEIIEIYFDYWINEHMYRKVLEKDEYYNDRCKEIKKVNKLLE
                     KNLSEEKKMDVLIELNDLFNSVIEIERELGYKLGFKAGARFMIELLNS"
     gene            complement(5546..6880)
                     /locus_tag="ACGI59_002700"
     CDS             complement(5546..6880)
                     /locus_tag="ACGI59_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFG9740480.1"
                     /translation="MDNMSNLVKNTEAEQSILGSILLDSRKIEEIEILISEDMFYHQE
                     HKHIFNSVKDIHKRDEPIDIITLTEDLKKKGLIDYVGGISYLTKLSTIVPTIGNVSHY
                     IEIVIEKYKNRNIIDKFNKFKLGEIDDNTLIYQLEKYVNEVNQKLVKKDSSITAISER
                     AFEYINDEIEEGIDIGIKFLDETIGGLYSGELITIAAKSGRGKTALALQIMRNVVFQG
                     KKVLFISGEMSDIQILFRNIASLTGISVVKMKNRNLNEEEIIKYINALSTLNQENNLY
                     INDSVNTISGIKREIKSIKPDVVIIDYLQILDYEGKEVGEAKFADLSRQIKKITLDFN
                     IPIIQLAQLNDELKDERPKGDRVMRSSKQIYMDSNSVIYIHRPTEKEALDYCKTMGIE
                     DIKELRRREKGFGANLSEIIVAKNRDGESGFRPYWYIGSLFSFKECKKVKEV"
     gene            complement(6893..7636)
                     /locus_tag="ACGI59_002701"
     CDS             complement(6893..7636)
                     /locus_tag="ACGI59_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9740481.1"
                     /translation="MSNLGWIKLHRKIKESPLYKDCNSKQRDILINLLLSVNHKPNKW
                     VYGGKEYEVKSGQLITSLESIANMCSKDVSIQNVRTALKKFERYGFLTNKSTNKNRLI
                     TIENWELYQSNEENQQTNQHAINKQLTTNKNVNNEKSKIYSEIIEKFNITCTELSKVK
                     KLTEARKKVINARIKEYDKETVLEIIHIVSKTSFLNGSNEKGWKANFDWIMSPNNFIK
                     ILEGNYQDKSINVKVDAEAYSPDDSQLKW"
     gene            complement(7657..7971)
                     /locus_tag="ACGI59_002702"
     CDS             complement(7657..7971)
                     /locus_tag="ACGI59_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9740482.1"
                     /translation="MADTNNLEVLKTLQATLNILVKQEQEAQEKEKGYKEIQTYRRDK
                     AQLITCKEASKIYPIGESKFRQLCHSKNKGFPCIWIDNRVYIIKNKLDNWFIENANGI
                     KL"
     gene            complement(7975..8169)
                     /locus_tag="ACGI59_002703"
     CDS             complement(7975..8169)
                     /locus_tag="ACGI59_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFG9740483.1"
                     /translation="MKLKLKRIEKGIKQYELAEKVGISRYYLSSLERGKSKNPSNMLM
                     LKLAQELDTSVEELFFDKEE"
     gene            8309..9169
                     /locus_tag="ACGI59_002704"
     CDS             8309..9169
                     /locus_tag="ACGI59_002704"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFG9740484.1"
                     /translation="MIDNTIGKKIKQYRKSINMSRDELANKIGCSVYAVAKYEQGQRK
                     PSLDMIKKISTALSIPINRLIIDESDLDTSTNNYTNLKELDLVVKTFDNYSAKLIGNN
                     ISKFRNINNISLKELSEKINCSENEIINYENGTKMPRIEILKKIALATYTKIEDLFGF
                     PLEEIKIDNQTILYRNPQTGYIIDNFLIDFINKDINIDNTPVSYYIDKSEFDLVIHIL
                     KNYGYDVKTDYKTKFEIADNADTIKPISLSFSDFKDFSKNLNWVINGLVENFIQENSN
                     GLIEDEDEEY"
     gene            9266..10018
                     /locus_tag="ACGI59_002705"
     CDS             9266..10018
                     /locus_tag="ACGI59_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HFG9740485.1"
                     /translation="MNKSYIPKYTHKFPTNLPHGDKKYTLTYIRRMISEFVYDMGNLE
                     NNPFTFPEVQTLLDGITVGGHKLSDQNQILNIKSSWDYVIKLSNKENLSLNKDTICSI
                     HKIVAKDEALIVGDFRNGNIGIAGTTQYECIEATLLNDLFVSDINIINKITNPLEKAI
                     IINLWLSYCQFFYDGNKRTARLTSNLILISNDIGVLSIPAKHKQEYNTLMLNFYETLE
                     ADEVIKFLLEKCITFFDEYNYKSYKELFKNGN"
     gene            10008..11174
                     /locus_tag="ACGI59_002706"
     CDS             10008..11174
                     /locus_tag="ACGI59_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFG9740486.1"
                     /translation="MATKRANGEGSIGKYKDGWRSRIMIGYNEDGKPIRKEFYGKTQK
                     EVKDKLDAYKKQYYLSSDISDDKITLEHWFYNWLFEYRIKDLKPKSFERYESIYRNYI
                     KDTDLGNVKLKDLRVSHIQKYYNRLLDSNKSIPTIRQINTKLKTCLSEAEKQGLIQRN
                     YCTMVNIPVEKKENKLEILTLEQQKSFIKAIDGHKLEVLFLLALGTGLRLGELLGLKW
                     FDIDFKKSNLTVKRTLQRTYFIDKTGNRELKVLEQEPKTSNSYRTVPIPKDVLNKLKE
                     HKVNQNSDILKAGELYRNDNYVFCNELGAPIDDKRPLRNLKSILNSLDIEPIKFHGLR
                     KTYATRLFENDVPPKTVQVLMGHYDISITLNIYTQVMEDKKIEAVEKLDKIFSL"
     gene            complement(11291..11521)
                     /locus_tag="ACGI59_002707"
     CDS             complement(11291..11521)
                     /locus_tag="ACGI59_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9740487.1"
                     /translation="MEANKTLLQSLYKKIILEFSKQTGKSLEESMDYLYTSETYKLIS
                     EGISDMHCKGHIYLAQELMLENGLMYHKGYPR"
     gene            complement(11770..16341)
                     /locus_tag="ACGI59_002708"
     CDS             complement(11770..16341)
                     /locus_tag="ACGI59_002708"
                     /inference="COORDINATES: protein motif:HMM:NF024685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HFG9740488.1"
                     /translation="MTFEEIMEFNKPYNQKNFDELVNAIKDGYVVPYIGAGMSMLFKD
                     IYPSWSTFLNNTSEEYLEDEDKILFEKIEDYEKKAEFLYEELAKVRFSKHLKKVFGAG
                     YLDAKDKDDFIDKCVYLIPIIFGSGLIITTNYDKVLEKNYGLHNKTVLVSHPGHFEAL
                     NNALRNNELVLYKIHGDITEPTTSIVLTKEQYEKAYSNDELRKMLTQVYTSKELLFLG
                     CSLEKDRPLELLQQVSEAGTGHYAIIPVSSEKKKHRRIELENEYYIQTIMYPEGKHEC
                     VRIILERILEIVLPDEYRRLKDRSLKESRLTNLSCRLTEKWFQNQNNIQIKNLGDRYL
                     PELNVELSLENTFNALGKNEEFKQRFDEKTDKVLSSIKKQKINEVEVDVLVIEEIIQQ
                     FYDKANERLDIKLLKKHFEKISAALDDRINNFKNIIINEDCEKSKKREDEIYKLKQTQ
                     KDIYVYIKYLDSKEIKVVNSPYILLHSAGGMGKSHIIAHTIEKRNMDGHKSILFLGQH
                     FKLDKTPIKHMLEILEIECEFEELLEILNEIGEKEQQRVILFIDALNEGQGKELWKYY
                     LNGIVEQIKKYPWIGLILSIRSEYIPSLIDNNNKIKENLVFIEHQGFSSIEHKAMKKY
                     FDYYGIPYSNIPMPNQEFRNPLFLRIMCETFKGEEINLDTISFTDIYKNYLNILNLRI
                     SESCNYSRHANVVTEVLNEMIKYKYGDGLGNNLIPANKSVEIIINIASKYKINNVIID
                     ELIANGIVNKTVNLNDEEYMHVTYEKLEDYLYANLLVKELKEIGVVDFSHKYKYLVEY
                     EDILEVLAIVLSEETGSDIPNEIFEVFKNKETNYNVISAFINALKWRKKSTITEKTLD
                     YINKIVLGKSYYKIDFYNVLILISTKVNHTLNADYIVKYILQNTMADRDAIFIPIFNE
                     VYEEEESSIKRLLGWCFDFEGFDNTSNETIRLSAMMIATFLISSNRDLRDKSTKALVN
                     LLTGKMDIVIDLLKTFEDVDDPYIIERLYAVAFGCVVSEQSEEMIEALAVYVYNKIFD
                     VDYVYPNILLRDYAKNIVDYAKYKIDSSVLKDMNVEPPYKSIFPKVPTDEEIEGYKYD
                     YKASDFKDYYWSQNTILNSMEVEYTRDGQPGWYGDFGRYMFQHYFHKWKGLDYNDLKN
                     IAIKKVFDMGYDVNKHGKFDRTVQNGRLRDSKYERIGKKYQWIALYELAAQAADNYKI
                     EVYTDAYGTKKEIYCKGTFEPDIRNIDPTVMTVINNNNNKRVHNQLFQFSPIPNNEWL
                     GDFSDLPSINDMVNSVYKNQDFLLLNGWYTWKEENDFKNKNYKNPEKDMWVLINSYII
                     KNDQLEPIVNALKGKDFMGRWLTEPNSNYTLYNKEYYWADGYYFFQNSYYCGEEWVQI
                     DEHKNILEKDYKVLLPTYSYTTEREGDIFQEDFSGTWHKPCSTLFYGLKLRYGQGDSI
                     LYNDYGEVVCFESSELLNEEIGFFINKEIFLKYLNEQGYSVFWTMLAEKRVLEEPYMD
                     RKYIQPTISGVYTLNGENRLVGETIESEY"
BASE COUNT         5619 a         2693 c         1653 g         6602 t
ORIGIN      
        1 tactagactt tttcaaaata aatatctaat tatttaaatc aataccaata taatttataa
       61 atggatttat aattgtatct tctatcatgt ttagtgtaaa caacttattt taatttaaat
      121 tcgtaaccaa caaagtacca atctgactta taattctata ttagtacaac tatggaaaaa
      181 tttaattcta tatctcctca cttaatctta tgacttctct taaatctttg taataatcct
      241 ctatattcat ttccatacat tgctttctat tttcctcagt tctactatga tcccaataaa
      301 ctccaaaatt ttgtatagtt gacactaagt ttatacattt atcaaaccaa tctttaattt
      361 caattggtgg aaaatctaaa tttataactt tattgtaaca ctcatccatt tttttctttc
      421 tactatctat aatttgaaaa atattatctt cacatatttt cttattttca tattcattat
      481 agaaggatat aatttcttca gatatgttta tcatatttgt taaaatttct ttaacttcgt
      541 atactgcctt ttctttagtg attgtatttt gacttatata tgattttaag ctttcgtagt
      601 tatcatatgt ttcagtgtga acaccgttga taaaaaattt atttttccca tttacactat
      661 accatatatc tttctcttga tatttatcaa tccaagtagt tttacacata tatattccat
      721 ttatcatatc aatttcatct ttgccaaaat atacccatat tatattagca tcttttcctt
      781 ttaatctatc ttctcctatt tcactattat aaacttcaac agacttaact atgtcaaaaa
      841 cctccttaga tatttctgat atatcacaat aggagaattt attttttaat ataacttcta
      901 ctgaatatct ttttattata cttatatgta aagatttgac attgtatatt atgtaatcgt
      961 catgctcttg aaaaactttc ttttttatat aagatttata atcttttaca ttaaaatata
     1021 tatctcccag tatgttagac ctaaagatag gattttgttt acagtttata taatttttgt
     1081 tcaatatact catacgcaaa ttaatatact tgcttattgc tcttaaagat aactcccctt
     1141 ttcttactat gtattcattt cttaacgctt ctgataataa acttgtaaat ctactaattg
     1201 aactatctgg aagagaaaat gattcttgat attcgctact tgatactaac actgcacatc
     1261 cttttctatc aaacaaggaa aatatgtctt taatatttat tttttgtttg tcacttacat
     1321 taaatttacc tgcaaagcaa caatcaataa atacaatttt atttttacaa tttaatcctt
     1381 ctataaaatc aattacaaat tgaatagaaa ctaattcatc acttaatact aagtaatgca
     1441 ctccgttatt acatatgcat ccatgccctg aaaagtaaaa tataaatgta tcatcatctc
     1501 ctatagaatc gcttatttct tgaaaagttt taaaaaataa atcttttctg acttcatact
     1561 ccatacctaa agttcttata ttattcgaag taatccccag tcttttatgt aacacattat
     1621 taatttcaat tatatcattt ttacaaaata gtaaatcttt atttttaact atactatatt
     1681 tacttactcc tacaattaat gcttttaagc ttttcatatt ccattccccc tttataattt
     1741 ttcaatttaa tatacttaaa atgatgtttt aattgtttta ttgattttat aaaagctagt
     1801 atcacactac ttttttgact ttttcattat ttacttttgt agttggatta taatttttca
     1861 aggtaagtat atatatattt atttacatcg cacccatata atttatatct ctgatttatc
     1921 tgacctgaac ttagtgtaat ttaaaccaat gtaaaatcat ctatctcttc accatatatt
     1981 ttttaaaatt ccccaaaata ttgatgttaa tagaatttca tgtgtttttt gtattctact
     2041 atccataaga attttaaagt gagtaaatat ttccaatcat ttatatctta tttaatatca
     2101 ttttattccc tcaagtattt atttaaattg ttatgcttta tttataaaat tatacataca
     2161 tttctaaagt tagacataca tctttaaaaa cctttaattt gtgctacttt ttttctaaaa
     2221 ataataaata tataaaaatt atatatacaa aaaattaagt tatgactagt ttataaataa
     2281 gtacataata aaattaaaga gagcaatttt ttttgctctc tttcaaaatt tatattaaat
     2341 tgtaataata aaaataaaaa cgctagttat ctaaggctaa tattttcaac gattatataa
     2401 atatctacat aacagttaaa ttacatactc ttcttcagtt attgcaactt cctcagtgac
     2461 atatattttt tctattttat taagataatc tactatactt aatccaatag cctttcctag
     2521 cttaacagga actgcatttc ctatctgctt gtacacctga ctaactgacc ctgtaaactc
     2581 ccactcatca ggaaaacttt gaattctagc atactctcta gttgtgaatg gtctagtctc
     2641 atctggatga cacctttcag tttgcttttg tgcaggacta catgttaaag ttaggcatgg
     2701 ttcatcccaa cttattcgcc ttgccatacc tctttttccg ccaccgctat aataactttt
     2761 tcccatataa tctcttgcca cctcatctgg caaatctatc caacacccac ctggtggtac
     2821 catatctaac acttttttct tcctctcagg atatctagct ccttccgatt ttggaacatt
     2881 tttaagtgca tctcttaacg tagcaacata ttcaaacgac ttagggaact taaaatccac
     2941 atgtgacaaa tcttttcttg ttcctattat aataactctt tccctctttt gtgcaactcc
     3001 atagttcaca gcctttagta tctcatattt tacattgtat ccaatctcgc taaatacatc
     3061 tatcatagtc tttaatgttt ttccaccatc atgagataca agacctctaa cattctctgc
     3121 taaaaacacc ttaggcttta actcttctag aattttagca tatgaataaa acaatgtccc
     3181 tcttacatcc tctaacccta gcttcttacc cgcatagcta aatgcttggc atggataacc
     3241 acctgatagc aaatctatct ctatattatt atcaatataa ttctttattc cattttccgc
     3301 tattttaaca atgtcaccct gtataacatt ccagtctggt ctatttaatt ttaatgtatc
     3361 acaagcatat ttatcccatt ctactaatcc aacctcttta aaaccagctt tttcaaggcc
     3421 taatgctaat cctcctgctc ctgcaaataa ttcaacacat gtatatgctc ttttagctag
     3481 tacattactt atatctatat gtttattatt tttatttctc tctctaggat ttaattcata
     3541 ttcactgagt tctttatatg tacagtctct atgctctata taatctttat tactgctaca
     3601 aatatttgta aatttcagtt gcttttcatt tacatcttcc aaacacataa tttcatcgaa
     3661 tccaatatct aacacttcac ataatttttc tacattactt tttataggtg taaatttttc
     3721 tgataatatt gcagataact gattcttagt tatgcccata tttaatgcca gttgattctg
     3781 acttcttata tttttctctt tcataatttt tctcactcta tttttatcaa tagttatttt
     3841 tttcatttag ttcacctctt aataaataat atacccttaa atagttaata aaatacatta
     3901 actatttaag gatatattat cataattcta atttttatgt aagtagttat aaaaacttaa
     3961 aataaaatat ctgtaaacaa cctaatatta cctcataatt ttacacatta tggaatatat
     4021 tgtataatta cattaatact ttttaggggg attattttat gactgaatta gaaaaacaag
     4081 aaatactaga taaagccaaa gattttttta caaatatttt ggttgctaat cataaaaaga
     4141 atactattaa gttaaattta aaggcattta atataaatcc ttttttagtt aaatacttag
     4201 ctagtttctt aacaggatca aataaacctg aagatattgc taaagcattg atttatccaa
     4261 gagttctagg cacttcgatt aatacgtcct tcggaactaa tttacaaaaa ttctgtatta
     4321 ccactttaga tggttttgcc tctacaactt ctggcattga tatagagttt attgattgta
     4381 ttgatggtag aaggaaattc tgtcaaatta aagctggacc aaacactatt aataaagacg
     4441 atgttaaaac tataattgac cactttagct cagttaaaag acttgctaga acaaatggac
     4501 tttcattatc ttataatgac ttaattgttg gtgtttttta tggaacagaa aatgagctta
     4561 atgctcacta taaaagaatt aatgaagagt atccagtagt tgttggtcaa gatttttggt
     4621 ataggcttac tggtgatgaa aatttttatt ttgatttaat agatacaatt gctcaatctg
     4681 ctttagaaga ggactgtact gttttactta aaaaaacaat atccaatcta gctgatgaaa
     4741 tttctaaaga taaatcttta ttcaatcaaa agtaatataa aaatgattaa atagcataat
     4801 ttacattttt agatatttaa aaataatatt caccaaacaa aattattaag taaaatgaaa
     4861 tgtatcttat aaaagctgag ctataagtaa atctttgtaa aaacaaattt acttatagct
     4921 tttttatgtt caaaaaacac aaattatata ctatttaata tattataaac ttttatttgt
     4981 taatactctc tactaaattt ctaacatctc ttatataaat taactattta ataattcaat
     5041 cataaatcta gctcctgcct taaaaccaag cttataacca agctctcttt caatctctat
     5101 aactgaatta aataaatcat ttaactctat caatacatcc attttctttt cttctgataa
     5161 atttttttct agtaatttat taactttctt aatttcttta catctatcat tataatactc
     5221 atctttttct agtactttcc tatacatgtg ttcatttatc caataatcaa aatatatttc
     5281 tataatttca tcatacatgt tagtacctcc ttgactttca tctggtactt aatatatact
     5341 taacttgtgt agattagtgt acattaagta cctaatttat aaaggtagtt gttatattta
     5401 tatggcaact acctttttct aataaatttt tcaatctaaa tactatatat tttaaatctt
     5461 cattgcttac tattgaggat tatatacttt tcttctaaca ctccttaaac aatagtttat
     5521 atatcactta ataaattata ttttttcata cctcctttac cttcttacat tccttaaaag
     5581 aaaacaaact tcctatatac caataaggtc taaagcctga ttccccatct ctatttttag
     5641 caactataat ttcacttaaa ttagctccaa aacctttttc tctacgtctt aattctttta
     5701 tatcctctat tcccattgtc ttacaataat caagagcctc tttctctgta ggtctatgaa
     5761 tatatatgac tgaattagaa tccatatata tctgttttga acttctcatt actctatctc
     5821 cttttggcct ttcatccttc aattcatcat ttaattgagc caactgtata attggaatat
     5881 taaagtctaa agtaatcttt tttatttgcc ttgataaatc agcaaactta gcttctccaa
     5941 cttctttacc ttcgtaatcc aaaatctgaa gataatcaat tataacaaca tctggtttaa
     6001 tactttttat ttctctctta attcctgata ttgtattgac tgaatcatta atatagagat
     6061 tattttcctg atttaaagta cttaaagcat ttatgtattt aattatttct tcttcattta
     6121 gatttctatt cttcattttt acaactgata ttccagttaa agaagctatg tttctaaaca
     6181 aaatttgtat atcactcatt tccccactta taaaaagtac ttttttacct tgaaatacca
     6241 catttctcat tatctgtaag gctagtgctg tttttcctct tccacttttg gctgctattg
     6301 taattaattc accactatat agacctccaa tagtttcatc taaaaatttt attcctatat
     6361 cgataccttc ttcaatttca tcatttatgt attcaaaggc tctttctgat atagctgtta
     6421 tactagaatc cttcttaacc aatttctgat taacttcatt aacgtatttt tctaattgat
     6481 atataagtgt attatcatct atttcaccta atttgaattt attaaattta tcaattatat
     6541 ttctattttt atatttttca ataactatct caatataatg ggatacatta cctatagttg
     6601 gaactattgt tgatagtttt gtcaaataac ttatacctcc aacataatct ataagcccct
     6661 ttttctttaa atcttctgta agagttatta tatctatagg ctcatccctt ttatgtatat
     6721 ctttcactga attgaatata tgtttgtgct cttgatgata aaacatatct tcacttatta
     6781 atatctcaat ctcttctatt ttcctagaat ctagtaaaat actacctaat atagattgtt
     6841 ctgcttctgt gttcttaact aaattactca tattatccat aatataaact ccctaccact
     6901 ttaattgtga atcatctgga ctatatgctt ctgcatctac tttgacatta attgatttat
     6961 cttgataatt gccttctaat atcttaataa aattatttgg gctcattatc caatcaaaat
     7021 ttgccttcca acctttttca ttgcttccat ttaaaaagct agttttacta actatatgaa
     7081 taatttctaa tacagtctct ttatcatatt cttttattct agcattaatt actttttttc
     7141 tagcttctgt aagtttcttt acttttgata gttctgtaca agtaatattg aatttttcta
     7201 taatttcact gtatatttta cttttttcat tatttacatt cttgttagtt gttagttgct
     7261 tgttaattgc atgttgatta gtctgttgat tttcttcatt agattgatat agctcccagt
     7321 tttcaatagt tataagccta tttttgtttg ttgatttatt tgttagaaat ccatatcttt
     7381 caaatttctt taaagcagtt cttacattct gaatacttac atcttttgaa cacatatttg
     7441 ctatactttc caaagatgtt attaactgtc ctgatttaac ttcatattct ttacctccat
     7501 aaacccattt attaggctta tgattaactg acagtaatag gtttatgagt atatctcttt
     7561 gtttggaatt acaatcttta tatagtgggc tttcttttat ctttctatga agctttatcc
     7621 aacctagatt actcaaatac tatcacccac ttaagtctat aattttatac catttgcatt
     7681 ttcaataaac caattatcta gtttattttt tattatatac actctattat ctatccatat
     7741 acaaggaaat cctttatttt tagaatgaca taactgtcta aattttgatt cccctatggg
     7801 ataaatttta gaagcttctt tacaagtgat aagttgagct ttatctcttc tatatgtctg
     7861 tatctccttg tacccttttt ccttttcttg tgcttcttgt tcttgcttga caagtatatt
     7921 caatgtagct tgtagtgttt taagaacttc tagattattt gtatctgcca tacatcattc
     7981 ctccttatca aaaaataatt cttctacact tgtatctagt tcttgagcca attttaacat
     8041 caacatatta cttggatttt ttgatttacc tctttctaaa gaagataaat aatatcttga
     8101 gatgccaact ttttcagcta attcatattg ctttatacct ttttcaattc tttttaattt
     8161 gagtttcatt gatttcctcc tttctttcat acccattttg tgtatcctca tttctataaa
     8221 atattctaac acccatttgg tgtattgtca atatatttaa gtgtatcaat agacccattt
     8281 agtgttataa tgtatttgag gtgaaattat gatagataat acaattggaa aaaagattaa
     8341 acaatataga aaatctataa atatgtcaag ggatgagtta gctaataaga ttggttgctc
     8401 agtttatgct gtagctaaat atgagcaagg ccaaagaaag cctagtttag atatgataaa
     8461 aaaaatatct actgcattat caattcctat aaataggctt attattgatg aaagtgattt
     8521 agatacttca acaaataatt atactaattt aaaagaattg gatttagttg taaaaacatt
     8581 tgataattac tcagcaaaac tcatcggaaa taatataagc aaatttagaa atataaataa
     8641 tatatctctc aaagaactct cagaaaaaat aaattgttct gagaatgaaa ttataaatta
     8701 tgagaatgga acaaaaatgc ctagaattga aatattaaaa aaaatagcac ttgctactta
     8761 tactaagatt gaagacttat ttggatttcc attagaagag ataaaaatag ataaccaaac
     8821 aatcctctat agaaatcccc agactggata tattatagat aactttctta tcgattttat
     8881 aaataaagat attaacatag ataatactcc agtttcttat tatatagata agtcagaatt
     8941 tgatttagta attcatatat taaaaaatta tggttatgat gtaaaaacag attataagac
     9001 aaagtttgaa attgcagata atgcagatac aataaaacca attagcttaa gcttctctga
     9061 cttcaaagac ttctccaaaa atctaaattg ggtgataaat ggtcttgttg aaaattttat
     9121 acaagaaaac tctaatggtc ttattgaaga tgaggatgaa gaatattagt attgcacatt
     9181 ttcaaaatat gagtttacct attaactttt atacattcat agttaataaa taaaaattag
     9241 atatgatatg aaaggagcta aaatcatgaa taaatcatat ataccaaaat acacacataa
     9301 attccctact aatctacctc atggagataa aaaatatact ttaacataca ttagaagaat
     9361 gataagtgaa tttgtatatg atatgggtaa cctagaaaat aaccccttta cattcccaga
     9421 agtacaaaca ttacttgatg gtataaccgt aggtggacat aaattaagtg accaaaatca
     9481 aatactaaat ataaaatcat catgggatta tgtaataaaa ttaagtaaca aagaaaactt
     9541 atctttaaat aaagatacaa tttgctctat acataaaata gttgcaaaag atgaagctct
     9601 aatagttgga gattttagaa atggtaacat aggtatagct ggtactactc aatatgaatg
     9661 catagaagcc actctattaa atgatttatt tgtaagcgac ataaatatca taaataaaat
     9721 aactaacccc ttagaaaagg ctataataat aaatttatgg ttgtcctatt gccaattctt
     9781 ctatgatggc aacaaaagaa ctgctagact tacctcaaac ctaatactta taagtaatga
     9841 tataggagtt ctatcaatac cagctaaaca taaacaagaa tacaacacat taatgctaaa
     9901 cttttatgaa acactagaag ctgatgaagt tataaaattc ttattagaga aatgtattac
     9961 attctttgat gaatataact ataaatcata taaggagctg tttaaaaatg gcaactaaaa
    10021 gagctaatgg agaaggctct atcggaaaat ataaagatgg ttggcgttcc cgtataatga
    10081 ttggctataa tgaagatggt aaaccaataa gaaaagaatt ttatggtaag actcaaaagg
    10141 aagttaaaga taaacttgat gcttataaaa aacaatatta tttaagctct gatatttcag
    10201 atgataaaat aactttagag cattggtttt acaattggtt atttgaatat agaataaaag
    10261 atttaaaacc taaatcattt gaaagatatg aaagcatcta tcgtaattat attaaagata
    10321 ctgatttagg caatgttaaa ttaaaggact taagagtatc tcacatacaa aaatattaca
    10381 atagattgct tgattccaat aaaagtatac ctactataag gcaaataaac actaaactga
    10441 aaacctgctt atcagaagct gaaaaacaag gtcttataca acgtaattat tgtactatgg
    10501 taaatatacc agttgagaaa aaggagaata agcttgaaat attgactctt gaacaacaaa
    10561 agagttttat aaaagctata gatggacata aattagaggt attattttta cttgctcttg
    10621 gtactggact tagattagga gaattattag gacttaaatg gtttgatatt gattttaaaa
    10681 agtctaattt aacagttaaa agaacccttc aaagaacata ttttatagat aaaaccggaa
    10741 atagagagct taaagtatta gagcaagagc caaagacatc aaattcttac agaactgtac
    10801 ctattccaaa agatgtttta aataaattga aagagcataa agtaaatcaa aattcggata
    10861 tattaaaggc tggagaatta tatagaaatg ataattatgt attttgcaat gaattaggtg
    10921 cccctattga tgataaaaga cctttaagga atctaaaatc tatattaaac agcttagata
    10981 tagagcctat aaaatttcat ggtcttagga aaacttatgc gactagactt tttgagaatg
    11041 atgtccctcc taaaactgta caagttttaa tgggtcatta tgatatatca ataactttaa
    11101 atatatatac tcaagttatg gaagataaga aaattgaagc tgtagaaaag ttggataaaa
    11161 tattttcatt gtaaaaatca aatatataaa aatactctga tttcatatag ttaattacaa
    11221 aaccaaaata aggatacaaa ttaattttat attatcttgt atccttattc ttgtctagtt
    11281 ttttaaatat ttatcttgga tagcctttat gatacattaa tccattttct aacattagtt
    11341 cttgggctaa atatatatga cccttacagt gcatatctga tataccctca cttataagtt
    11401 tatatgtttc agaagtatat aaataatcca tactttcttc caagctctta cctgtttgtt
    11461 tagaaaattc taatatgatt tttttatata gcgattgtaa caatgtctta ttagcttcca
    11521 taaaagctat acctcctctt tcaaatattt gtttatccta ttttgactaa ctatatattt
    11581 attagttatt taattagcca taataattcg gctataagat atttataatc taatctcaat
    11641 tatataaatc tttttgccaa tatttaataa tcaaaatatt gcacttaatt aactatctta
    11701 taagtattga taataaaatc tgtcttctta aatatactta aataaaactt ctataattat
    11761 atcaaagtat taatattcac tctctattgt ctctcctaca agtctatttt ctccatttag
    11821 ggtatatact cctgatatag ttggttgtat gtattttcta tccatatatg gttcttctaa
    11881 aactcttttt tctgctagca tcgtccaaaa tacactatat ccttgttcat taagatattt
    11941 taagaaaatt tccttattaa taaagaatcc tatctcttca ttaagtaatt cactactttc
    12001 aaaacaaaca acttcaccat aatcattata tagaatcgaa tctccctgac catatctcaa
    12061 tttcaaccca taaaatagag tactacaagg tttatgccat gtgccagaaa agtcttcttg
    12121 aaatatgtca ccttctcgtt cagtagtata actataagtt ggcaataata ctttataatc
    12181 cttttctaag atattcttat gttcatctat ttgtacccac tcttctccac aataatatga
    12241 attttgaaaa aagtaatatc catctgccca ataatattcc ttattataaa gagtataatt
    12301 actattaggt tcagtaagcc atctacccat aaagtctttt ccctttagag cattcactat
    12361 tggttctagt tggtcatttt taattatata gctattgatt aatacccaca tgtctttttc
    12421 aggattttta tagttcttat ttttaaaatc attttcctcc ttccaagtat accatccatt
    12481 aagtaataaa aagtcttgat ttttgtaaac actatttacc atatcattta tgcttggtaa
    12541 atcactaaaa tctcctaacc actcattatt aggtattgga gaaaattgaa ataattgatt
    12601 atgtactctt ttattgttat tattatttat tactgtcatg acagttggat caatattcct
    12661 aatatctggc tcaaatgtac ccttacaata aatttctttt ttagtaccat aagcatctgt
    12721 atacacttct atcttataat tatcagcagc ttgcgctgca agttcatata atgcaatcca
    12781 ttgatatttc tttcctattc tttcatattt actatctctt aatctaccat tttgtactgt
    12841 tctatcaaat ttaccatgtt tatttacatc atatcccata tcaaaaactt tcttaatagc
    12901 tatattcttt aaatcgttat agtctagacc tttccattta tggaaatagt gttggaacat
    12961 atatcttcca aaatctccat accatcctgg ttgtccatct ctagtatatt ctacttccat
    13021 agaatttaag atagtgtttt gactccaata ataatcttta aaatctgatg ccttatagtc
    13081 atacttatat ccctcaattt cttcatcagt tggtacttta ggaaaaattg atttatatgg
    13141 tggttcaaca ttcatatctt ttaatactga actatcaatt ttatattttg cataatctac
    13201 aatatttttt gcataatctc ttaataaaat attgggataa acataatcta catcaaatat
    13261 tttattataa acataaactg ccaatgcctc tatcatctct tctgattgct ctgatacaac
    13321 acatccaaat gcaactgcat ataatcgttc aataatatat gggtcatcta catcttcaaa
    13381 tgttttaagt aaatctatta caatatccat tttccctgtt aatagattta caagagcttt
    13441 tgtactttta tctcttaagt ctctatttga agaaattaaa aacgttgcaa tcatcatagc
    13501 tgacaatcta attgtttcat ttgaagtatt atcaaaacct tcaaaatcaa agcaccatcc
    13561 taataatcgc ttaatagaag actcttcttc ttcataaact tcattaaata taggtataaa
    13621 gatagcatct ctatctgcca tagtattttg taaaatatat tttactatat aatctgcatt
    13681 taatgtatga ttaacctttg tagaaattaa gataagtaca ttataaaaat ctattttata
    13741 ataagattta cctaaaacta ttttattaat ataatctagt gttttttcag taattgtaga
    13801 tttcttacgc cattttagtg cattgataaa tgcactaata acattataat ttgtttcttt
    13861 atttttaaaa acttcaaaaa tttcattagg tatatctgag cctgtctctt cagataaaac
    13921 aattgccaat acttctaata tatcctcata ttctactaaa tatttatact tatgtgaaaa
    13981 atctactacc ccaatttctt ttaactcttt aaccaacaaa tttgcatata aataatcttc
    14041 taacttctca tatgtcacat gcatatattc ttcatcattt aaattgactg ttttattaac
    14101 tattccattt gctatgagtt catctattat cacattgtta atcttatact tacttgcaat
    14161 atttattata atctctacac tcttgtttgc gggtattaaa ttattcccaa gaccatctcc
    14221 atatttatat ttaatcattt cattaagtac ttctgtaact acattggcat gtctactata
    14281 attgcatgat tcagatattc taagattaag tatattcaaa taatttttat aaatatctgt
    14341 aaaactaata gtatctaagt taatctcttc tcctttaaaa gtttcacaca taattctcaa
    14401 aaaaagaggg tttctaaatt cttgatttgg cattggaata ttagaataag gtattccata
    14461 ataatcaaaa tactttttca tagctttatg ttctatacta gaaaatcctt gatgctctat
    14521 aaaaaccaaa ttctctttta tcttgttatt attatctatt aaagatggta tatattcact
    14581 tcttattgat aatattagtc caatccatgg atatttcttt atctgttcta caataccatt
    14641 taaataatat ttccaaagtt cttttccttg accttcattc aatgcatcta taaataatat
    14701 cacacgctgt tgctcttttt ctcctatttc attaagtatt tctaataatt cttcaaactc
    14761 acactcaatt tctaatattt ctaacatatg tttaattggt gttttatcca acttaaaatg
    14821 ttgccctaaa aataatatag acttatgccc atccatattt cttttctcaa tagtatgtgc
    14881 aattatatga gatttcccca taccacctgc actatgtaat aaaatataag ggctattaac
    14941 tacttttatt tccttagaat ccaaatattt tatatataca tagatatctt tttgagtctg
    15001 cttcaatttg tatatttcat cttctctttt tttacttttt tcacagtctt catttataat
    15061 tatattttta aaattattta ttctatcatc aagtgctgca cttatttttt caaagtgctt
    15121 ttttaacaac ttgatatcta atctttcatt agctttgtca taaaattgtt gtattatttc
    15181 ttctataact agtacatcta cttccacttc atttattttc tgtttcttta tacttgatag
    15241 aactttatct gttttttcat caaaacgttg tttaaattct tcattttttc ctaatgcatt
    15301 aaacgtattt tctaaactca attctacatt caattctggt aaatatctat ctccaaggtt
    15361 ttttatctgt atattattct gattttgaaa ccatttctct gttaatctac aagataaatt
    15421 agttaaccta ctttccttta aagacctatc ctttaatctt ctatattcat ctggcaaaac
    15481 tatttccaaa attcgttcaa gtattattct tacacactca tgcttacctt caggatacat
    15541 aatggtttgt atataatact cattttctaa ctctattcgt ctatgtttct ttttctcact
    15601 actaactgga ataatagcat aatgacccgt tcctgcctct gatacttgct gtaataattc
    15661 caacggtcta tccttttcca aactacatcc aagaaataac aactctttac ttgtatatac
    15721 ttgtgtaagc atctttctca attcatcatt actatatgct ttttcatatt gttcttttgt
    15781 taacacaata gatgttgtag gctctgtaat atccccatga attttataca atactaattc
    15841 attatttcta agtgcattat ttaatgcttc aaagtgacct ggatgtgaca ctaaaactgt
    15901 tttattgtgt aatccataat ttttttctag tactttatca taattagtag ttataattaa
    15961 tcctgagcca aaaattatag gaattaaata gacacattta tcaataaaat cgtctttatc
    16021 ttttgcatcc agataacctg ccccaaaaac tttttttaaa tgtttagaaa atctaacttt
    16081 cgccaattct tcatataaaa attctgcttt tttctcataa tcctctattt tttcaaataa
    16141 aattttatcc tcatcttcta aatattcttc acttgtgtta tttaaaaatg tactccatga
    16201 aggatatatg tctttaaata acatagacat gcctgctcca atatatggga ctacataacc
    16261 atctttaatt gcatttacca gttcatcaaa atttttttga ttgtaaggct tattaaattc
    16321 cataatttct tcaaatgtca ttattccctc ctaaactatc tcttaataat ttcaaccata
    16381 attcctattt tataatttta ttatatcatt ccattccatt tttttactac aattctatgt
    16441 tatagaatga attcacttac ttacatttgt taataatata aaactttctt ttaagataat
    16501 taaaatttat acttttaaat tatggataaa aatggaatgt tataactttt aatgtattac
    16561 taatatt
//
LOCUS       DAYAPA010000091        16565 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.091, whole genome shotgun sequence.
ACCESSION   DAYAPA010000091 DAYAPA010000000
VERSION     DAYAPA010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16565
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.091"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            107..775
                     /locus_tag="ACGI59_002709"
     CDS             107..775
                     /locus_tag="ACGI59_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HFG9740489.1"
                     /translation="MSKLESTTEKVEVKWYGYVALILGALFFSGIFKDAPGPLKVLDF
                     NNVLGSFGTLGTINDGVGTLAANFRGDGGTGPRDGWLYALTLIPSVMFALGVVRVIDH
                     LDGMKAAQKLLSPLLKPLLGLPGFAGLTLIASLQSTDAAASMTKELKDDGYIDEKQKA
                     VFCAFQFSGASAITNFFASGAALFPFIGDVPIFIPLALILIMKFVGANLLRLYLNKFD
                     KGEA"
     gene            777..1262
                     /locus_tag="ACGI59_002710"
     CDS             777..1262
                     /locus_tag="ACGI59_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HFG9740490.1"
                     /translation="MSEVAKKEVKVKKGNVIDAFIGGARNGFQISTNSMAPNVIFGFA
                     IISVFNLTGLLDVIGTIFTPVMSIFGLPGVAATAIMTIFISMGGASGVIAGLFTAGQI
                     SQAHVAILLPMLLLSGAQIQFMGRLLGTAELKTKYYPHMFVIATLNGCIAMLIMNYFV
                     V"
     gene            1323..2444
                     /locus_tag="ACGI59_002711"
     CDS             1323..2444
                     /locus_tag="ACGI59_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 peptidase aminoacylase family protein"
                     /protein_id="HFG9740491.1"
                     /translation="MGTNKDYIEYVHKVYDYLHTIPEKGFEEYKTSEFVLKELEKMGF
                     ENINKGVAGTGIIVTIDSKKEGPVFAIRADMDALEFIIDGEKKMIHACGHDGHTSMLL
                     TAAKLIKERNLVKRGKLKILFQPGEEKLYGALRVIDSGLLDDVEEMVGMHLRPGQDCR
                     LGKAIPAMYHCASCIMEFTIKGITCHGSKPHLGVNAVDAVCLAVNAINAIRVNPAIPN
                     SIKVTNISVKGETYNNIPDEAFMALDIRAESNDIIEELKEKAIHAVKTVVSSINAEAV
                     LISKDGVPAAEFDEELVNLAEETIKDVLGADASIGIFKNSGGEDFHYYTQKLKCKTTY
                     IGLGADATPGFHNPNVTFDTKALEYGVDIWCRLVEKRLG"
     gene            2725..3915
                     /locus_tag="ACGI59_002712"
     CDS             2725..3915
                     /locus_tag="ACGI59_002712"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFG9740492.1"
                     /translation="MSKYNFDRVIDRVGTDCVKWDFRTNCSTKAQKDGLPFWIADMDF
                     ECAEPIIEALRKKVEHKIFGYSSNDSDKYFNAVCDWYKRRFNWEISRKDIIFSPGVVP
                     AVAILVRILTNSNEGVIVQKPVYYPFEAKIKSNNRDVVNNPLIYENGTYRMDYDDLEE
                     KAKCSNNKVLILCSPHNPVGRVWREDELKKVVEICKKYDLWIIADEIHSDLIRKGFKH
                     TPLQSLCPEYKDKIVTCIAPSKTFNLAGMQLSNIIVNNDELKKKYQEEVTAVGVATSP
                     NPFAIVATIAAYNESEDWLNELNDYLDNNIQFIDEYLKENLPKVKLVYPEGTYLAWLD
                     FTAYGLNEVELEDLMFKKANVLFDEGYIFGKEGIGFERINVACPQSLLKECMDRLKTI
                     FENL"
     misc_binding    4049..4304
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            4363..5364
                     /gene="asnA"
                     /locus_tag="ACGI59_002713"
     CDS             4363..5364
                     /gene="asnA"
                     /locus_tag="ACGI59_002713"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HFG9740493.1"
                     /translation="MSTIIPKDYKSSLNVIDTQIAIKKLKDFFEMRLSNELNLIRVSS
                     PLFVLPETGANDNLNGEKAVSFDIPFMNKNAEIVQSLAKWKRLALKKYGFEVGSGLYT
                     DMNAIRKNEELDNIHSLYVDQWDWELVIDKSSRNEKTLKDVVKRLYGVFKDTEIFVCS
                     MYEGIKEILPEEITFITSQELEDMYPELSPKEREDKIVKEKKAVFITQIGKTLISGEK
                     HDGRSPDYDDWELNGDILFYNPVLDSALELSSMGIRVDEESLDRQLKLAGCEERKEFD
                     YHKMLLNGELPYTIGGGIGQSRICMYFLQKAHIGEVQVGVWPQDMIQNCSSAGIELL"
     gene            5468..5857
                     /locus_tag="ACGI59_002714"
     CDS             5468..5857
                     /locus_tag="ACGI59_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HFG9740494.1"
                     /translation="MEFLKIIINIVLDILKKILVRFKNAKFGLVFVFDLLKLPDFMTD
                     KRINIVDKIKVISVLIFTISYFVSGVDIIPEMIAGAFGFIDDAIVLIWSIGIVNEEIN
                     KYRVIAKKDKHSNIIENVEFSIKDEEE"
     gene            6018..7226
                     /locus_tag="ACGI59_002715"
     CDS             6018..7226
                     /locus_tag="ACGI59_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HFG9740495.1"
                     /translation="MKKKIGLVPKLIIGIIVGILIGSYAPEIIVQILVTVSTLFSAFL
                     KFVIPFIIIGFVTAGIADLATGAGKLLGITTGIAYGSTLIAGLLSFAVSTLIFPNFID
                     ASVASQIGDPEAGMLAPIFTIPLEPMVDVTAAIVFAFVMGLGISALRNHGKGETLFNF
                     FQEFQGIVTKTLSTVIIPLLPLYIAGTFANITIAGEVWTILGVFWKVYLVVIPLHFVY
                     MACQFTAAGVFSGKNPVRMLKNQVPGYLTAIGTQSSAATIPVNVGCAEKNGVSKQIRE
                     FVVPLCATIHLSGSMISITGFTTAVLMMNGMPHGIGVLFPYIAMLGIAMVAAPGAPGG
                     AIMSALPFLPMVGIPSDGGLASLMIALYLTQDSFGTACNVSGDNAIAVVVDKINDGMS
                     KKGNKEEQSA"
     gene            complement(7312..8388)
                     /locus_tag="ACGI59_002716"
     CDS             complement(7312..8388)
                     /locus_tag="ACGI59_002716"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003415975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFG9740496.1"
                     /translation="MSTNHGANLYSLSSKYGFSKEEFMDFSSNINPFGTSSLAKQYIV
                     NNIDMVSMYPDPDYIDLKTSISNYCKCSIDNIVLGSGATELISSFIHTINPKQALLLS
                     PAYSEYEKELSKINCSIEKYFAKEEDNFHINLENLIKTINAKDYDLVVICNPSNPTGF
                     AFTKVEVREILKNTDSFLMIDETYVEFTDTDTYSCTQLVDDYSNLFVIRGTSKFFSTP
                     GIRLGYGLISNTNVKNEINKNLDLWNINIIASKMGEIMFSDLDFISNTISLMNTERDY
                     LLKELKNIKSLDIYNTKGNFILCKIKTKELTAKSLREQLLPQKIIIRDCCSFEGLDEY
                     FFRVCILKPNENKLLISSLKAIFK"
     gene            8617..9144
                     /gene="hpt"
                     /locus_tag="ACGI59_002717"
     CDS             8617..9144
                     /gene="hpt"
                     /locus_tag="ACGI59_002717"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFG9740497.1"
                     /translation="MYKVTGKMLTEEQIKEKVYELGKKIEEDFKGEDLLVVGILKGAS
                     VFVSDLIRCIDLDVNIDFMSVTSYGNSTESSGTVKILKDLDVDIEGKNVLIVEDIIDS
                     GLTLSNLVAALKTRNPKSLKLCTLLDKPQRRKANIPVDYVGFVIEDKFIVGYGIDYAE
                     KYRNLPYIGIVEDVE"
     gene            9233..10165
                     /locus_tag="ACGI59_002718"
     CDS             9233..10165
                     /locus_tag="ACGI59_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFG9740498.1"
                     /translation="MDIQLNILLFFQNLRNPILNFIFLVFTISTEAPLLILITTIIYW
                     CINKKCGQKILFAIIGNFVVNLGIKEFVKAPRPIGIKGLESLRVSTAGGYSFPSAHTQ
                     TATTFWVSIMTIFKKRYLHLIGAVMVLGVGLSRLYLAVHWPIDVIAGWILGIFFTVVF
                     VKIFDYIDDNKNYILLVVLLIPFIIVAIFLNSPEYFEKFGIIVGFVFGYMVEDRFVKF
                     NTDNNNKKINFSNKNKGSKNIIFSRICRFLVGIVTIGMLYIGIKLFISMLIVTLNISD
                     STMSIIFMNFIKNTVVVFYGIAGAPALFKLLKLN"
     gene            10227..11453
                     /locus_tag="ACGI59_002719"
     CDS             10227..11453
                     /locus_tag="ACGI59_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFG9740499.1"
                     /translation="MKKVVIIGGGPAGMIAASTACEKGYDVTLIEKNHKLGKKLAITG
                     KGRCNITNACEIEELIENVPTNGKFLYSAFYTFTNDDVISMFNNLGVKTKTERGKRVF
                     PESDKAFDIVNALERQLKSKKVNILLNSKVEKIISKNNKIEKVILNDKKEIKCDSVVV
                     ATGGLSYPLTGSTGDGYKFAISQGHTIIDTKPSLIGIEVQESFTKDLEKLSLRNVEIR
                     VFNSKQKKVYSDFGELEFTKFGLDGPIIKSASCRMKDTRKENYTILLDLKPALDEEKL
                     DKRVQKDFQKYTNKKFEKALDDLLPKKLIPIIINLSEINPNTVVHQISREQRKNLVHL
                     LKNLKFTVKRYRPIEEAIITSGGVKVNEINSSTMESKLVEGLFFAGEVIDIDAYTGGF
                     NLQIAFSTGYLAGFNC"
     gene            11537..11758
                     /locus_tag="ACGI59_002720"
     CDS             11537..11758
                     /locus_tag="ACGI59_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small, acid-soluble spore protein, alpha/beta
                     type"
                     /protein_id="HFG9740500.1"
                     /translation="MASNNNNNRTVVPGAKEALNQMKLEIANELGMSNYQQVDKGNLT
                     ARENGYVGGYMTKKLVEMAEQQMAGKSNR"
     gene            11947..12117
                     /locus_tag="ACGI59_002721"
     CDS             11947..12117
                     /locus_tag="ACGI59_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9740501.1"
                     /translation="MSSVNFEDLKNKFINSDLDEKIRIYTTTEGLSVEQFKELLKYYP
                     IQHLSKLEKALG"
     gene            complement(12210..12671)
                     /locus_tag="ACGI59_002722"
     CDS             complement(12210..12671)
                     /locus_tag="ACGI59_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9740502.1"
                     /translation="MQIEQLDLETRNKIYCYTKKILRKYQKGITSGKLTADKFADNIL
                     SQDFISSILNEKIVNETNFKISYRNYIETLINIQNENLSNLRKKSTKTAKCFNISQIT
                     QLKNLLSNTGYNLLIPYKYLTARDIEGIVTLINTGSIELGNERIYNYISKV"
     gene            12861..13322
                     /locus_tag="ACGI59_002723"
     CDS             12861..13322
                     /locus_tag="ACGI59_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9740503.1"
                     /translation="MLNGIIKLFYSEIVKTKINNLDSSGYSLRDECKEIRNITLKIDS
                     VFFKLLKLAFGSILLMVAFNLNMVLGIGVFLVGVVYILYKRNMEEQVKEHINNVKNNI
                     DISKITFMDEKGRTGLNALITLLLIGLLTGFNWILVASFVVVFVFTIKNVC"
     gene            13365..15128
                     /locus_tag="ACGI59_002724"
     CDS             13365..15128
                     /locus_tag="ACGI59_002724"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing SpoIIE family protein
                     phosphatase"
                     /protein_id="HFG9740504.1"
                     /translation="MTNGSYKMKNLVEISAMVTESTDFFNIKDDIIEKMLEVVHPTKA
                     CVNLFYKNDSKYAYLVCSQTLEYVPQIFPINSLKGAKIDFDTYPEYIHEAVNEKKIIY
                     VENVFEDSRAEGERDLAKAEGYIGRIVFPFIINNVVVGFMTCFLKEDDYITEQDIDFI
                     SSVASLLSLSIEITNKNNNVQILIDKLRGSISAINEATKKLYLNKSIDGFLENLSKQA
                     CNITNSKEALIIINSHHNKDAIVSCYNVEQKKKTNLYPMIDMFLKKESLGGYLNNLKP
                     CSKKGINLESYIYYKLQDKNDTIGCIVCANSKNYTSDDLNILSILSKQVSVGMQLYEH
                     NEKEVKHKVLENELNILNKQQQLIMDKGKMDCNDEKELYFYHKPARVVGGDFYYATKV
                     DDEHIVYIVADVMGHGMVSNYVVAMIKGAFKVLCNQYNTPREIMTNLNRMLYDEFDKM
                     GVFTTCLIGIIDTKENMITVSNAGHYSPVVVKKDGTIENNLNCKKGIPIGIMEDATYE
                     NNTLSMEDYAMVCMYTDGILEIKNSSKEEYGINRLENFLKENFRLNQQLIVENLKLDL
                     KNFSSKDNYDDDILIVMLKDR"
     gene            15136..15744
                     /locus_tag="ACGI59_002725"
     CDS             15136..15744
                     /locus_tag="ACGI59_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HFG9740505.1"
                     /translation="MDSNDKKGLKSPEIIIINGSPRINKNCSFIANEVKAMLEECGLS
                     CKIFNIYNMDIEYCTACGFCEKTGYCKFNDDMKPLYKMFDDSKGTIVISPVHFDSISA
                     KLKTVVDRTQAIYASKYVLNKPSIDRSKKRIGMYIAVGGSKSYDSQFKGGQIVMDFFF
                     KSINTKLLHNIYLNNTDDVSYIDNQEFKDIIYKNLKIYIDSI"
     gene            15816..16385
                     /gene="pduL"
                     /locus_tag="ACGI59_002726"
     CDS             15816..16385
                     /gene="pduL"
                     /locus_tag="ACGI59_002726"
                     /EC_number="2.3.1.222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HFG9740506.1"
                     /translation="MKLPIALSNKHVHLSQADIDVLFGAGHELTKFKPLSQPGQYACE
                     EKVDLVGPKGTLKGVRVLGPARAHTQVEISLADGFPLGVKAPIKESGDIAGTPGVKLV
                     GPAGEVEMKEGVIVASRHIHMSNEDAAKFGVTDKQIVKVKTSGPRALVFENVLVRASD
                     SFNLEMHVDMEEGNAAGVKNGDLVELIVD"
BASE COUNT         6476 a         1680 c         2930 g         5479 t
ORIGIN      
        1 aataaaaaaa taaatacaaa ggctttatca ttaaggtatt ggcataagtt ttgcttatta
       61 aacaagtata ctaataaaat tcatttaaac aggagggaga atttatatga gtaaattaga
      121 atcaacaaca gaaaaagttg aggtaaaatg gtatgggtat gtagcattaa tattgggagc
      181 attatttttt tcaggaatat ttaaagatgc accaggacca ttaaaagtat tggattttaa
      241 caatgtatta ggttcttttg gaacattggg aacaattaat gatggtgtag gtacattagc
      301 tgcaaacttt agaggtgatg gaggtactgg ccctcgagat ggatggctat atgcattgac
      361 attaatacca tcagttatgt ttgcacttgg agtagttaga gttatagacc acttagatgg
      421 aatgaaggca gctcaaaagc ttcttagtcc actattaaag ccattattag ggttacctgg
      481 atttgcagga ctaactttga tagctagttt acagtctaca gatgcagctg catctatgac
      541 taaagaactt aaagatgatg ggtatataga tgaaaaacaa aaagctgtat tttgtgcatt
      601 tcaattttca ggtgcgtctg caattacgaa tttctttgct tctggagcag cactatttcc
      661 atttatagga gatgtaccaa tatttatacc acttgcttta atattaatta tgaagtttgt
      721 tggggcgaac ttacttagat tatatttaaa taaatttgac aagggggaag cttaacatga
      781 gtgaggtagc aaaaaaagaa gtaaaagtta aaaaaggaaa tgtgatagat gcgtttattg
      841 gaggagctag aaatggattc caaataagta caaattcaat ggctccaaat gtcatcttcg
      901 gttttgctat aatttctgtg ttcaacttaa caggtctttt ggatgtaata gggacaatat
      961 ttacacctgt aatgagtatc tttggacttc caggtgttgc agcaacagct ataatgacaa
     1021 tatttatatc aatgggagga gcatctggag taatcgcagg tttatttaca gcaggtcaaa
     1081 taagtcaagc acatgttgca attctattgc caatgttatt gctttcagga gcgcagatac
     1141 aatttatggg gagactttta ggaacagctg agttaaaaac taaatattat ccacatatgt
     1201 ttgttatagc aactttaaat ggttgtattg caatgcttat aatgaattat ttcgtagtat
     1261 aggtaattat aataaaaatt taatataaat ggaaagttta aattttaagg aggagctaga
     1321 ttatgggaac taacaaagat tatattgagt atgtacataa agtgtatgat tatctacaca
     1381 ctataccaga aaaaggattt gaagaatata agacatcaga gtttgtttta aaagagcttg
     1441 aaaaaatggg gtttgaaaat attaataaag gagttgcagg aactggtatt atagtaacta
     1501 tagattctaa aaaagaagga ccagtatttg ctataagagc agatatggat gcattggaat
     1561 ttatcataga tggagaaaag aaaatgatac atgcttgtgg tcatgatggt catacttcta
     1621 tgcttttaac agctgcaaag ttaataaaag agagaaattt agtaaaaaga ggtaagttaa
     1681 aaatactttt tcaaccaggt gaagaaaaat tatatggagc attaagagta attgattctg
     1741 gtttgttgga tgatgtagaa gaaatggtag ggatgcattt aagacctgga caagattgta
     1801 ggttgggaaa ggcgatacct gcaatgtacc attgtgctag ctgtataatg gaatttacga
     1861 ttaaaggtat cacttgtcat ggttctaaac cacatttagg agtaaatgca gtagatgctg
     1921 tctgtttagc agtgaatgca ataaatgcaa taagagtaaa tccagctata ccaaattcta
     1981 taaaagtaac aaatatatca gttaagggag aaacttataa taatatacca gatgaagcat
     2041 ttatggcttt agatataagg gcagaaagta atgatataat agaagaatta aaagaaaaag
     2101 caatacatgc tgtaaaaact gttgtaagtt ctataaatgc agaagctgta ctaatttcca
     2161 aagatggagt accagcagca gaatttgatg aagaacttgt aaacttggct gaagaaacta
     2221 taaaagatgt attgggagct gatgcatcta taggtatatt taaaaattct ggtggagaag
     2281 acttccatta ttatacacaa aaactaaaat gtaaaactac atatattgga ttaggagctg
     2341 atgctacacc aggatttcat aatccaaatg taacatttga tacgaaagct ttagaatatg
     2401 gtgtagatat atggtgtaga ttggttgaaa agagattggg ataacatcca aactaataat
     2461 tctacattta aaattataat tatagaagtt atagaaaaag attgcttatg ttaattagaa
     2521 aataattaat atgagcaatc ttattttgat ataatgaaac tgattttgga atattgaaaa
     2581 aatatgatgg aaaagtaatt atattttaaa tctattggac aaaaaagtga tattattata
     2641 atattaagaa ttgtaaaata ataccaaagt gcatctagta atagacttaa caattataaa
     2701 aattttattc ttaggaggga aaaaatgagt aagtataatt ttgacagagt aatagataga
     2761 gtaggaacag attgtgtaaa atgggatttt agaactaatt gttcaacaaa agcacaaaaa
     2821 gatggtttgc ctttttggat tgctgatatg gattttgaat gtgcagaacc aataatagaa
     2881 gcattacgta aaaaagttga acataaaata tttggatata gttcaaatga tagtgataaa
     2941 tactttaatg cagtttgtga ttggtataaa aggagattta actgggaaat aagtagaaaa
     3001 gatattattt ttagtcctgg tgttgtacct gctgttgcta ttttagtaag aatattaact
     3061 aattctaatg aaggtgtaat agtccaaaaa ccagtgtatt acccatttga agctaagata
     3121 aagagcaata atagggacgt tgtaaacaat cctctgatat atgagaatgg tacttatagg
     3181 atggattatg atgatttaga agaaaaagct aagtgtagca acaataaagt actaatactt
     3241 tgtagtcctc acaaccctgt tggaagagtt tggagagaag atgaattaaa aaaggttgta
     3301 gaaatatgta aaaaatatga cctatggata atagctgatg agatacattc tgatttaatt
     3361 agaaaaggat ttaaacatac tcctttacag tcattatgcc cagagtacaa ggataaaata
     3421 gttacatgta tagctccaag taaaacattt aatctggcag gaatgcagtt gtctaatata
     3481 atagtgaata atgatgaact aaaaaagaaa tatcaagaag aggttacagc tgttggagtt
     3541 gcaacttcac caaatccctt tgctattgta gctacaatag cagcatataa tgaaagtgaa
     3601 gactggctta atgaattaaa tgattactta gataataata tacaatttat tgatgaatac
     3661 ttaaaagaaa acctaccaaa agtaaaactt gtttatccag aaggaacata tctagcatgg
     3721 ttagatttta ctgcttatgg attaaatgaa gtagaacttg aagatttaat gtttaaaaaa
     3781 gctaatgtat tatttgatga aggttatata tttggaaaag aaggaatagg atttgaaaga
     3841 ataaatgtag cttgtccaca gtctttatta aaagaatgta tggacagatt aaagactata
     3901 tttgaaaatt tataaagtat aggtaaaaat ttataaagtg tttaaaaata aaaaaataat
     3961 aaacttgaca aattttaact aatcacttat catactatta tgaataaata gaaaacataa
     4021 gagagctaaa ttttattgaa ttaaactctt aaagactttg aaaggaagag tagttataga
     4081 atgtaattaa agcgagttgg tgatggtgtg agaccaatat gaagtctata gtgaagaaca
     4141 tcctggagtt gctaaccgaa attttaaaag agtaggctta gtcggaacca aaccgttatc
     4201 atattggggt gtatcgagta atatctgtac attgtgagct gggtttgaat ttatttcaaa
     4261 ctaatcaggg tggtaccgcg gaaaagcaac ctttcgtccc ttttatatag ggatgaaggg
     4321 tttttttaat ggaaaaatat ataaatatag ggagtgaatg atatgagcac aataatacca
     4381 aaggattata aaagtagttt gaacgtaatt gatacacaaa tagctataaa aaaattaaaa
     4441 gatttttttg aaatgagact aagtaatgaa ttaaatctta taagagtttc ttctccgtta
     4501 tttgtattgc cagaaacagg tgcaaatgat aatttaaatg gagaaaaggc tgtaagcttt
     4561 gatataccat ttatgaataa aaatgcagaa attgttcaat cacttgcaaa atggaaaaga
     4621 ttagccctta aaaaatatgg gtttgaagtt ggtagtggtt tatatacaga tatgaatgca
     4681 ataagaaaaa atgaagaatt agacaatata cattctcttt atgtagacca atgggattgg
     4741 gaattggtta tagataaaag tagtagaaat gaaaagactc taaaagatgt tgtaaaaaga
     4801 ttgtatggag tatttaaaga tactgaaatt tttgtatgta gtatgtatga aggtataaaa
     4861 gaaatattac cagaagaaat aacttttata acatcacaag agttagaaga tatgtatcca
     4921 gaattatcac caaaggaaag agaagataaa attgtaaaag agaagaaagc agtatttata
     4981 actcaaatag gaaaaacatt aatatcagga gaaaaacatg atggaagaag tcctgattat
     5041 gatgattggg aattgaatgg ggatatatta ttttacaatc ctgttttaga ttcagcatta
     5101 gaattatctt caatgggaat tagagttgat gaagaatctc ttgatagaca attgaaactt
     5161 gctggatgtg aagagagaaa agagtttgac tatcataaga tgcttttaaa tggcgaactt
     5221 ccgtatacaa ttggtggagg aattggtcag tcaagaatat gtatgtattt cttacaaaaa
     5281 gctcatatag gagaagtaca agttggagta tggccacagg atatgattca aaattgtagt
     5341 agtgcaggaa ttgaattatt ataatttaaa tataaactaa ttgataaaat ttatatctaa
     5401 tatgttcatc aaattaatta tatcggttat aatgtagaat aacctataat tataagaggt
     5461 gataaatatg gagtttttaa aaataatcat taatatagtt ttagatattt taaaaaaaat
     5521 tttggtcaga tttaaaaatg ctaaatttgg tttggttttt gtatttgatt tacttaaact
     5581 acctgatttt atgacagata aaagaatcaa tatagttgat aaaataaagg taatttctgt
     5641 attaattttt actatatcct actttgtatc tggggttgat ataattccag agatgattgc
     5701 aggggcattt ggttttatag atgatgctat agttttaatt tggagtatag gtatagtgaa
     5761 tgaagaaatt aataagtata gagttatcgc aaaaaaagat aaacattcaa atataattga
     5821 aaatgtagaa ttttctataa aagatgaaga agaataacaa tttatatatg ttattctttt
     5881 ttttattaac aaaaaatggt taaattgttg acggagaggt aaaactctat tatcataata
     5941 agtggatatc caatatttaa ctaagtattc aatgtgcaag aatgcttaat ttaaaaaagt
     6001 tatggagggt aaatattatg aagaaaaaaa taggtttagt gcccaaacta atcatcggta
     6061 tcatagtcgg tattttaatc ggaagttatg caccagaaat aatagtacaa atattagtaa
     6121 cagttagtac tttattctca gcattcctaa aatttgttat accatttata attataggtt
     6181 ttgtaacagc aggtatagct gatttagcaa caggtgcagg aaagttattg gggataacta
     6241 cgggaatagc atatggttct accttaatag caggattatt atcatttgca gtatctacat
     6301 taatcttccc aaattttatt gatgcaagtg ttgcaagtca aattggagac ccagaggcag
     6361 gaatgttagc tccaatattc acaattccat tagagcctat ggtagatgta acagctgcaa
     6421 tcgtatttgc atttgtaatg ggacttggaa tatcagcttt aagaaatcat ggaaaaggag
     6481 agactttatt taatttcttc caagaatttc aaggaatcgt aacgaaaacg ttgtcaacag
     6541 tgattatacc tttattacct ttatacatag caggaacttt tgcaaatata actatagcag
     6601 gggaagtatg gacaatatta ggggtattct ggaaagtata cttagtagta atacctttac
     6661 attttgtata tatggcatgt caattcacag cagcaggtgt attttcagga aaaaatccag
     6721 taagaatgtt aaaaaatcaa gtacctggat atttaacagc tataggaact caatcttcag
     6781 cagcaactat accagttaat gttggttgtg cagaaaaaaa tggagtaagt aagcaaataa
     6841 gagaatttgt tgtacctctt tgtgcaacaa tacatttatc aggtagtatg atatcaataa
     6901 caggatttac aactgcagtt ttaatgatga atggtatgcc acatggaata ggtgtattat
     6961 tcccttatat tgcaatgtta ggtatagcaa tggttgcagc accaggagca ccaggtgggg
     7021 ctataatgag tgcacttcct ttcctaccaa tggttggaat accatcagat ggtgggcttg
     7081 caagtttaat gatagcttta tacttaactc aagacagttt tggtacagca tgtaatgtat
     7141 caggagataa tgcaatagca gtagtagtag ataaaataaa tgatggaatg agtaaaaaag
     7201 gaaataaaga agagcaaagt gcttagtttt ttaataatca aaaatgagtt atctcaaata
     7261 gagatagctc attttttatg gtataaaaaa ttataaaata aagtgatttt tttatttaaa
     7321 aattgcttta agagatgata ttaataattt attttcatta ggttttaata tacatactct
     7381 aaagaaatac tcatctagac cttcaaatga acaacaatct cttattatta ttttttgtgg
     7441 aagcagttgc tctcgaagag atttagcagt caattccttt gtcttgattt tacacaatat
     7501 gaaattacct ttagtattgt atatatctaa gctttttata ttcttaagct ctttaagcag
     7561 gtaatctctt tcagtgttca ttaaggaaat agtatttgat ataaaatcta aatcactaaa
     7621 catgatttca cccattttag aagctattat gtttatattc cataaatcta gatttttatt
     7681 tatttcattt ttgacattag tgttagatat aagaccatat ccaagcctta ttccaggcgt
     7741 tgaaaaaaat tttgatgtac ctcttataac gaataaatta gaataatcat caactaattg
     7801 agtacaagag tatgtatctg tatcagtaaa ctctacataa gtctcatcaa tcattaaaaa
     7861 tgagtctgta ttttttaaaa tttctctcac ttctacttta gtaaatgcaa atccagttgg
     7921 attactagga ttacagatta ctaccaaatc ataatcttta gcattgatag tttttataag
     7981 attttctaaa tttatatgaa aattatcttc ttcttttgca aagtattttt ctatagaaca
     8041 attaatttta gataactctt tttcatactc tgaataggct ggagagagta aaagagcctg
     8101 tttagggtta attgtatgta taaatgatga aattagttct gtagctccac tgccaagtac
     8161 tatattatct atagaacatt tgcagtaatt ggatatagaa gtttttaagt ctatgtaatc
     8221 agggtcagga tacatagata ccatatctat attgtttaca atgtattgtt tggctaaact
     8281 tgatgtacca aaaggattta tatttgagct aaaatccata aattcttcct ttgaaaagcc
     8341 atacttacta gataaactat ataaattagc tccatggttt gtgctcataa aagtacctcc
     8401 aaaaataaaa ttatataatc gataaaaata actgattaat atatgtacat atagattata
     8461 atacaaagga atcgttattc caaattttta atgtaaaaag caaaaaagta tggataagct
     8521 gttaatttag gtttaaaaat gatgaaaaag attttaattg atattttggt atgctataat
     8581 atataagtta atagaaggaa agcgggagga tacaaaatgt acaaagttac aggaaaaatg
     8641 ttgacagaag aacaaataaa agaaaaagtt tatgaattag gaaaaaagat agaagaagac
     8701 tttaaaggcg aagatttact agtggtaggt atacttaaag gagctagtgt atttgtttca
     8761 gatttaataa gatgtataga tttggatgta aatatagatt ttatgagtgt tacaagttat
     8821 ggaaactcaa cagagtcttc tggaacagtt aaaatattaa aagatttaga tgtagatatt
     8881 gaaggaaaga atgtgttgat tgtagaggat ataatagatt ctggtttaac attaagtaat
     8941 ctagttgcag ctttaaaaac tagaaatcca aaatctttaa aactatgtac tctacttgat
     9001 aaacctcaga gaagaaaagc taatatacct gtagactatg ttggatttgt tatagaagac
     9061 aaatttattg ttggttatgg aatagactat gctgaaaagt atagaaattt accttatata
     9121 ggtatagttg aggatgtaga atagatttaa atgtgaatca aaagcactaa tttctaaggg
     9181 agttagtgct ttttagttaa ttatataaaa ttttagtaaa ggagacatct atatggatat
     9241 tcagttaaat atattattat ttttccaaaa tttaagaaat ccaattttga actttatatt
     9301 tcttgtcttt acaataagca cagaagcacc gttattaata ttaattacaa caattatata
     9361 ctggtgtata aataaaaaat gtggacagaa aatactgttt gctataattg gaaattttgt
     9421 tgtaaattta ggcatcaaag agtttgtaaa agctcccaga ccaataggaa taaaaggtct
     9481 tgaatcattg agagtttcta ctgctggtgg ttattctttt ccaagtgctc atactcaaac
     9541 tgcaacaaca ttttgggtta gtattatgac tatatttaaa aagagatatc ttcatttaat
     9601 aggtgcagtt atggttttag gagtaggatt gtctaggctg tatttagctg ttcattggcc
     9661 aatagatgtg atagcgggtt ggatattagg aatatttttt acagttgtat ttgtaaaaat
     9721 atttgactat attgatgata ataaaaatta tattttactt gtagttttac ttataccatt
     9781 tataatagtg gctatatttc taaatagccc tgaatatttt gaaaaatttg gtataatagt
     9841 aggatttgta tttggatata tggttgagga tagatttgtt aaatttaata cagataataa
     9901 taacaaaaaa ataaatttta gcaataaaaa taaggggagt aaaaatataa ttttttcaag
     9961 aatatgtagg tttttggttg ggatagtaac aataggaatg ttatatattg gcattaagtt
    10021 gtttatttca atgttaatag tcactttaaa tataagtgac tctactatga gtataatttt
    10081 tatgaatttt attaagaata cagtagttgt tttttatgga atagcaggag ctccagcatt
    10141 atttaaactt ttaaaattaa attgatttta aggtatagag tcaatagttt aaattaaaaa
    10201 aattaagggt agaaaagagt gattatttga agaaggtagt aataataggt ggtggaccag
    10261 cagggatgat agccgcatca actgcatgtg agaaaggcta tgatgtaaca ttaattgaga
    10321 aaaatcataa attaggaaaa aaacttgcaa taacaggaaa aggtagatgt aatattacta
    10381 acgcttgtga aatagaggaa ttaatagaaa atgtacctac aaatggaaaa tttctttata
    10441 gtgcttttta tacttttaca aatgatgatg taataagtat gtttaataat cttggagtaa
    10501 agactaaaac agaaagagga aaaagggttt ttccagagag tgataaagca tttgatattg
    10561 taaatgctct tgaaagacaa cttaaaagta agaaagtaaa tatattatta aattcgaaag
    10621 tagaaaaaat aatatctaaa aataataaaa ttgaaaaagt aattttaaac gataaaaaag
    10681 aaattaagtg tgattcagtt gtagtagcaa ctggtggatt aagttatcca cttactggtt
    10741 ctactggtga tggatataaa tttgcaattt cacaggggca cacaattata gatacaaaac
    10801 catctttaat tggtatagaa gtacaagaaa gttttacaaa agatttagaa aaattatcat
    10861 tgagaaatgt tgaaataagg gtgtttaata gcaaacaaaa gaaagtttat agtgattttg
    10921 gagaacttga atttactaaa tttggtttag atggacctat aatcaagtct gcaagttgta
    10981 gaatgaaaga tacaaggaaa gaaaattaca ctatattatt agatttaaaa cctgcattag
    11041 atgaggaaaa attggataaa agagttcaaa aagattttca gaagtataca aataaaaaat
    11101 ttgaaaaagc attagatgat ttacttccaa aaaaactaat acctataata ataaacttaa
    11161 gtgaaattaa tccaaatacg gttgttcacc aaatttctag agagcagaga aaaaacttag
    11221 tccatttatt aaaaaattta aagtttacag taaagagata cagacctatt gaagaagcta
    11281 taataacttc tggtggtgta aaagtaaacg aaataaattc aagtacaatg gaatcaaaat
    11341 tagtagaagg tttatttttt gctggagaag taatagatat agatgcatat acaggtggat
    11401 tcaatttgca aatagctttt tctactggat atttggcagg ttttaattgc taaaaattac
    11461 ctgaattaaa aatgtttaaa atcacataat attatcagca acacaaaata aattaaacag
    11521 aaggaaggta atcaacatgg caagtaacaa taacaacaac agaacagtag taccaggagc
    11581 aaaagaggct ttaaaccaaa tgaaattaga aatagctaat gaacttggta tgagtaatta
    11641 tcaacaagtt gataaaggaa accttactgc aagagaaaat ggttatgtag gtggatacat
    11701 gactaaaaaa ttagttgaaa tggctgaaca acaaatggct ggaaaaagca acagatagtt
    11761 ataaataatt atttataact attggctatt agtttagcta gagctatctc aaatatagaa
    11821 agtaatttct aaattgagat agcttttttt tattaaatta ttaaatagat atatttttat
    11881 gtaataaatt ttatgtattt ggataaaata atacaaatga atatgttaca taaggagtga
    11941 tgtaaaatga gtagtgtaaa ttttgaggat ttaaaaaaca aatttattaa tagtgactta
    12001 gatgaaaaaa taagaatata tacaacaaca gaaggtttat cagtagaaca atttaaagag
    12061 ctactaaaat attatccaat acaacattta tctaaactag aaaaagcact tggatagcag
    12121 tatttaatcc aaaattaaaa aggcaattta tttaatatta aaaatatttt ttattaaata
    12181 aattgccttt ttataatagg tttaaattat tatacttttg atatataatt gtagattctc
    12241 tcatttccaa gttcaataga cccagtatta ataagagtaa ctattccttc tatatctcta
    12301 gcagttaagt atttatatgg aattaataaa ttataaccag tattacttag taagtttttt
    12361 aattgggtta tttgagagat attgaaacat tttgcagttt tagtagattt ttttcttaag
    12421 ttagataaat tttcattttg aatatttatt aaagtttcta tatagtttct ataagatatc
    12481 ttaaagtttg tctcattgac tattttttca ttaagtatac tactgataaa atcttgagac
    12541 aatatattat ctgcaaattt atctgctgtt aattttccag atgttatgcc tttttgatat
    12601 tttctaagaa tttttttagt atagcagtat attttatttc tcgtttctaa atcgagttgt
    12661 tctatttgca caaaaccact tccttctaaa ttataaaaat atttattaaa aatctatact
    12721 taactaatca agcttcaata acttatttac aataaatact aaataataaa atatttgtta
    12781 ttttttatta ttaccaagta taaaaaatat tataattgta aaacttacaa atatatgtaa
    12841 aatatttagg agtgatgaaa atgttaaatg ggataataaa gcttttttat tctgaaattg
    12901 taaaaacaaa gattaataac ttagattcaa gtggatattc tttaagagat gaatgtaaag
    12961 aaatcagaaa tataactcta aagatagata gtgtattttt taagctttta aagttagctt
    13021 ttggttcaat attgcttatg gtagctttta atttaaacat ggttttaggt ataggagtgt
    13081 ttttagttgg tgtagtttat atactatata aaagaaatat ggaagaacaa gtgaaagagc
    13141 atataaataa tgtgaaaaat aatatcgaca tctctaaaat aacttttatg gatgaaaaag
    13201 ggagaacagg tctaaatgca ttaattacat tacttcttat aggattattg acaggattta
    13261 actggatatt agttgctagt tttgtcgttg tttttgtttt tactataaag aatgtatgtt
    13321 aaaatactaa taaatagttt aatatatagg gggagaaatt gagtatgact aatggcagtt
    13381 ataaaatgaa gaatttagtg gaaatatccg ctatggtgac tgaatctaca gattttttta
    13441 atataaaaga tgacataata gaaaaaatgt tagaagttgt tcaccctaca aaggcatgtg
    13501 tgaatctatt ttataaaaat gattctaaat atgcctactt agtatgttcg caaaccttag
    13561 aatatgtacc tcaaattttt ccaataaata gtcttaaagg tgcaaaaatt gattttgaca
    13621 catatccaga gtatatacat gaggctgtaa atgaaaaaaa gataatatat gtggaaaatg
    13681 tatttgaaga ttctagagca gaaggtgaac gagatttagc aaaagctgaa ggttatattg
    13741 ggagaatagt ttttcctttt ataataaata acgtagttgt tggatttatg acttgttttt
    13801 taaaagagga tgattatata acagaacaag atatagattt tatatcatct gttgcatcac
    13861 tcttaagttt atcaatagaa ataactaata aaaataataa tgtacaaatt ttaattgata
    13921 aactaagagg ctcaataagt gccataaatg aagctactaa aaaactatac cttaataaga
    13981 gtattgatgg ctttttggaa aatttaagta aacaagcgtg taatattact aatagtaaag
    14041 aagctttaat aattataaat agtcatcata ataaggatgc tatagttagt tgttataatg
    14101 ttgagcaaaa aaagaaaaca aatttatatc ctatgattga tatgttttta aagaaagagt
    14161 cattgggggg gtatctaaat aatttaaaac catgctcaaa aaagggaata aatttggaaa
    14221 gttatatata ttacaaactt caggataaaa atgatactat aggatgtata gtttgtgcaa
    14281 atagcaaaaa ctatactagt gatgatttaa atattcttag tatattgtcc aaacaagttt
    14341 ctgttggtat gcaattatat gaacataatg aaaaggaagt aaaacacaaa gttcttgaaa
    14401 acgagttgaa catattaaat aaacaacaac agcttattat ggataaaggt aaaatggatt
    14461 gtaatgatga gaaagaactt tatttttacc ataaaccagc aagggtagta ggtggagatt
    14521 tttattatgc tacaaaagtg gatgacgaac atatagttta tattgttgct gatgtaatgg
    14581 gacatggaat ggtatcaaat tatgttgttg caatgataaa aggtgctttt aaagtattat
    14641 gcaatcaata caatacacca agagaaataa tgacaaattt aaataggatg ttatatgatg
    14701 aatttgacaa gatgggagta tttacaactt gtttgatagg tattatagat actaaagaaa
    14761 atatgattac tgtgtctaat gcaggtcact atagtcctgt agttgtaaag aaagatggca
    14821 caatagaaaa taatttaaat tgtaaaaagg gcatacctat tggaataatg gaagatgcta
    14881 cttatgaaaa taatactctt tcaatggaag attatgcaat ggtatgtatg tacactgatg
    14941 gaattttaga gataaaaaat tcatcaaaag aagagtatgg tataaaccga ttagaaaact
    15001 ttctaaaaga aaattttaga ttaaatcaac agttaatagt agaaaatcta aaattggatt
    15061 taaagaactt ctcatcaaaa gataattatg atgatgatat tttaatagtt atgttaaagg
    15121 ataggtagat tgattatgga ttcaaacgat aaaaaaggac ttaaaagtcc agagattata
    15181 ataataaatg gaagtccaag aattaataaa aattgcagtt ttatagcaaa tgaagtaaaa
    15241 gctatgctag aagagtgtgg attaagttgt aaaatattta atatctacaa tatggatatt
    15301 gaatattgca cagcatgtgg attttgtgaa aaaacagggt attgcaagtt taatgatgat
    15361 atgaagccat tatataaaat gtttgatgat tcaaaaggaa ctatagtaat aagtcctgta
    15421 cattttgatt caatatcagc taaattaaag actgttgttg atagaactca agctatatat
    15481 gcaagtaaat atgtattaaa taaaccttca attgatagaa gtaagaaacg gataggaatg
    15541 tatattgcag tgggggggag taaatcatat gattcacagt ttaaaggtgg acagattgtg
    15601 atggattttt tctttaagag tataaatact aaacttttac acaatatata tttaaacaat
    15661 acagatgatg tatcttatat agataatcaa gaatttaaag atataatata taaaaattta
    15721 aagatatata ttgattcaat ataatttgat tttgtaggta atattatgta taattgtatt
    15781 gcaataattt aaataattaa aaggagggaa tttcaatgaa attaccaata gcattatcta
    15841 ataaacatgt tcatttgagt caagctgata tagacgtatt atttggggca ggtcatgagt
    15901 taactaaatt taaaccatta tctcaaccag ggcaatatgc ctgtgaagaa aaagtggatt
    15961 tagtaggacc aaaaggaact ttaaaaggag ttagagtact aggaccagct agagctcata
    16021 ctcaagttga aatatcttta gcagatggtt tcccattagg agtaaaagca ccaataaaag
    16081 aatctggtga tatagctgga actccaggtg ttaaattagt aggacctgct ggagaagttg
    16141 aaatgaaaga aggagttata gttgcttcta gacatataca tatgagcaat gaagatgctg
    16201 ctaaatttgg tgtaactgat aagcaaatag taaaagttaa aacatctgga ccaagagcat
    16261 tagtatttga aaatgtatta gtaagagcta gtgacagctt taatctagaa atgcatgttg
    16321 atatggaaga gggaaatgca gcaggcgtta aaaatggaga tttagtagaa ttaatagttg
    16381 attaatatta taaatagact ttatgctagt agatatttta tttgctagca tatttttaaa
    16441 tgtaataaat ttctatagga taaatttaat cgattagatt aatttataaa ataaaggggt
    16501 attataaaaa atgtaataaa aacttacaaa aatatgatgt aatatggtaa aattatatat
    16561 ataaa
//
LOCUS       DAYAPA010000092        16476 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.092, whole genome shotgun sequence.
ACCESSION   DAYAPA010000092 DAYAPA010000000
VERSION     DAYAPA010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16476
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.092"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            74..349
                     /locus_tag="ACGI59_002727"
     CDS             74..349
                     /locus_tag="ACGI59_002727"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFG9740507.1"
                     /translation="MKKILVACGAGIATSTIVCDRVERLVKENNIQAEVEQCKIAECS
                     TKQEGADLIVSTTILPTTYDIPTIKATGYITGINTTALDKKILNALK"
     gene            592..2292
                     /locus_tag="ACGI59_002728"
     CDS             592..2292
                     /locus_tag="ACGI59_002728"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9740508.1"
                     /translation="MIKQFVKYYKPYKKIFTLDLIAAFLFSLCDLVYPMITRNIMDDV
                     VPNKNLRMLVVFAVALILIFIAKAGLNYFMQYWGHVIGVDMQADMRNEVFTHLQRLPN
                     TYFDNNKSGVTMSRIVNDLMDITELAHHGPEDLFISIVMLVGSFFILIDINIPLTLII
                     FAILPFIIWFAIAKKDKMNIAFMKSRVTIGDVNATLENSIAGMKVTKSFCTEKEELNK
                     FVRSNKLFRRARQDSYKVMAEYYSGMNLYMDILEWVVVIAGGYFTYIGKITLGDFAAY
                     ILYVKMFIQPMKKLINFTEQYQNGMTGFKRFIEIMEQDHQKEAKNPIELENVKGDIEI
                     ENISFTYEDKTQVLDNLSLSIKAGKTIALVGPSGGGKTTLCNLLPRFYEFDKGDIKID
                     GKSIKDVSLKSLRKNIGIVQQDVFLFTGTIRDNILCGNPNATDEEMIAAAKKARIHDF
                     VETLPDGYDTYIGERGVKLSGGQKQRISISRIFLKNPPIIILDEATSALDNVTEREIQ
                     ESLEELSKDRTNLVVAHRLTTIKNADEIIVLTDKGIEERGTHEELVNKNGVYSRLHNN
                     "
     gene            2655..3842
                     /locus_tag="ACGI59_002729"
     CDS             2655..3842
                     /locus_tag="ACGI59_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9740509.1"
                     /translation="MEAITAFCLVLVSLTIGDIVSAKTKAFVPSVFVSAIIFIVGFWT
                     FFPENIVDLAALGTPLAQLGMLLLITHMGTMMSIKELAGQWKTIVIALAGIVGICVGA
                     LALGTVVFGWDTAVIATPPLTGGLVASIMMADAATAKGLASLSVLAILMYVAQGFAGY
                     PITALMLKKEGKRLLSDFRSGKVTVNTDEEKVKDLPEQKSRFKIIPDLPEKYDTTYMI
                     ILRLGFVAWLAVQFTNLTNEVVSRYVICLIFGVIASELGIVDRKPLNKSGSFGWLMTS
                     LMAFIFAGLAKATPAMLVEMAVPLVGIIIFGVIGMGIFSIIVGKLLGESKEMAFSVSL
                     TSLYGFPPNYILTEEAVKALAETPEEKEFLMDKMLPKMLVGGFTTVTIASVVIAGIFV
                     NLI"
     gene            3902..5065
                     /locus_tag="ACGI59_002730"
     CDS             3902..5065
                     /locus_tag="ACGI59_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFG9740510.1"
                     /translation="MDIKEITKSYKDYVIKLRREFHENPEKSMEEVRTSKRVKEELDK
                     IGIPYVSAGGTGVIATIKGDNPGKTVALRGDMDALQVVECTDVEYKSKNEGLMHACGH
                     DGHTSMLLGAAKVLNDIKDSINGTVKLFFQPGEEVGKGARAMIQDGAMEGVDSVFGIH
                     LWTDVESGTISVEEGPRMASADFFKITVKGRGGHGSLPHQGVDAVLASSAIVMNLQSM
                     VSREVSPLEPLVVSVGVLNSGTRFNVIASEAILEGTIRLFNPELRKQIPGILERIAKS
                     TAEAYRADAELEYGYLTPAVINDKECSKIATDAAIKLFGEDCITLFEKVTGAEDLAEF
                     MNIAPGALAFVGARNESKGACYPHHHGCFNIDEDALEIGTALYVQYAVDFLNK"
     gene            5216..6019
                     /gene="zupT"
                     /locus_tag="ACGI59_002731"
     CDS             5216..6019
                     /gene="zupT"
                     /locus_tag="ACGI59_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HFG9740511.1"
                     /translation="MNNVIFAFLITLLAGLSTGIGSCIAFFAKKTNTKFLSISLGFSA
                     GVMIYVSMIEIFPKAQDALTKSMGEKLGSWSTVIAFFIGMAIIAVIDKLIPQEENPHE
                     IKKMENIEEKNIKKNKSLLRTGIFTAMAIAIHNFPEGLATFISALDDVTIAIPIAIAI
                     AIHNIPEGISVSVPVYYATGDKKKAFYYSFLSGMSEPLGAIIGYVLLRNFLNDITLGI
                     VFAIVGGIMVFISLDELLPSAREYGEHHLSIYGLIAGMGVMAISLLLFK"
     gene            6123..6674
                     /locus_tag="ACGI59_002732"
     CDS             6123..6674
                     /locus_tag="ACGI59_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HFG9740512.1"
                     /translation="MEKKIKFKFDWYSSPDKIEKWLEDIEAKGYNLYKINKFGGIFYF
                     HKGSPRKIKYTVDYYYDVENNYFDEHKSNGWNLVFRSIGSFMGTSAWYIWSKEYKDIP
                     PKLYSDSDKTNKVKSILFMNMFIFFMLVVIFIYLLNIEIRNLYSGYGASVTAIVLQII
                     LLILYAVQLYRRLMYYLRLKKFK"
     gene            6924..7637
                     /gene="rgbR"
                     /locus_tag="ACGI59_002733"
     CDS             6924..7637
                     /gene="rgbR"
                     /locus_tag="ACGI59_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RgbR"
                     /protein_id="HFG9740513.1"
                     /translation="MIKIAVCEDEKETQLLIEDYLENILKDISIEYEIQKYISGEELL
                     ESNLKDIDILLLDIKMEKLNGMDTARKIREVDNEMEIIFVTSLIDYVQEGYEVRAYRY
                     LLKPIELEELKKHVLTCIKDIEINKESHITIKNKSNTYKIYLNEIKYIEVQKKDMLIH
                     TINKNFDIKYSLGKIEKELNPYKFIRCHKSFIVNLRYVENIKPNTAILESGEEVPISR
                     YRYKEVKEKFLKFLGDTIC"
     gene            7631..9010
                     /locus_tag="ACGI59_002734"
     CDS             7631..9010
                     /locus_tag="ACGI59_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFG9740514.1"
                     /translation="MLNFMTELLVVLSIFIQFSIMNGILIYKKDSKKNEKIVFYIVLF
                     LIYIIIQISRLFNGGNYFSNINYLFYILAFIAWGGYYNYFYKMGYIKYSIFFYLFSVY
                     YKCLDAVIYRILFFAITGNVISSKESLLVPYLESIKIQIYSNLIIILIYLIFSFFRKS
                     IKLISQDKRNYVYLLFALLVNTMNMLVNYVLKKLERFGSLHSEGYYFDNFVNPKLVGA
                     SSIFLILLFKEIIKENRLKSQAELIKNKLDMQYAHYLSIQESHMRVKKLYHDINNHIY
                     CIDNLRNNSKEINEYVNNLKDEIKTFKYIYNTGNMILDIIINEKSEVCLKKGIKFTCS
                     INFSKVNFVKPIDVSSIFSNILDNAIEACDKIVDENINKYIRIKGTITRSFFVLKCEN
                     SKLNQLTFNKNILLTNKMDKFVHGIGIQSIKSSLQKYNGELLFENSIDKFILNIYIPL
                     DQDTDSWGD"
     gene            9251..9940
                     /locus_tag="ACGI59_002735"
     CDS             9251..9940
                     /locus_tag="ACGI59_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFG9740515.1"
                     /translation="MKVKRNINRVAIIVALNLSLALMVGCMSPVSDKNLSSSEQISKD
                     SLKEQVEDKKDESIELFNNYSQKINDAKGREEVKKDLSQRFSKAIIDSGYSITNSKSD
                     GSFVVSINGAEKPPEKNIRSLFYSMSEDDKEGKVTIDILCAKEYPEDDKLSESDKYVK
                     FIYNLFKSLTDTNLTEKEIFSMVEEDFNKGVGVVELPYMKDIHVEVNKVKQSTKVLKL
                     SLVYKFDISNH"
     gene            10161..12533
                     /locus_tag="ACGI59_002736"
     CDS             10161..12533
                     /locus_tag="ACGI59_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase/glycerol dehydratase
                     family glycyl radical enzyme"
                     /protein_id="HFG9740516.1"
                     /translation="MSQTNRIEAFRQEYINSKPMICCERARIFTESHKKTEGEAICIR
                     RAKAFLETCKELPIKIFENELIVGTAGKFRRTGILTPEFSWQWVDKEMDTFDKRTQDP
                     YVISKEQIEFIRKEIFPYWKGKSLEEVFLARIPEDTAKILVDTGIIDNDSKWRQAVGE
                     VTPDYQDILFVKGYKGIKEDADKKIKELDISVSENIEKIDFYKSVSIVAQGIMTLAQR
                     YSNLAKEMSKQETDEKRKLELIKISEICMNVPANPPTNFYEAIQFVWFVQLGGILSEN
                     PLALNLGRFDQYMYPYYENDAREGKITESEAQELIEALWIKLSEWVWTISANTANYFA
                     GYNQFQNLTVGGKKRNGTDGTNDISYMCLKATESVKTHQPGLSVRVSQGAPDNFVMAV
                     AKLVKQGTGFPAIHSDSAGAQMLLQDGYDAEDARDWSNCGCVVPHFRKTGQWTSAVNI
                     NFAAALEYAMNEGKSRLTGEKMGLDTKNITEFTSFEELKDEFLKQLAYLVKSSVIGTT
                     VAQQIHKEMVPRPFLSTCVDGCLDKGVDLSKGGAKYNIGPVLTGIGLGVVSNSLAAIK
                     KLVFEDKVTTLEELTKALNNDWEGYEELRKLALDVPKYGNDNDYVDSLAIEVSDFYYT
                     ETRKYKDIFGSKFNSAFMGISNYVPTGKIVGATPCGRKATKPLTEGVSPFVGTDTTSP
                     LAAMKSASKINHDVHTGGTLLNLRLNQDLVETERGLRNLTSMIKSYFALGGFHVQFNT
                     ISNDTLLKAQENPEEYKDLLVRVAGYSTQFVNLSREMQDAIIARNSHSNF"
     gene            12548..13450
                     /locus_tag="ACGI59_002737"
     CDS             12548..13450
                     /locus_tag="ACGI59_002737"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HFG9740517.1"
                     /translation="MSKKGRVVKVQHFSVNDGDGIRTTIFLEGCKLKCKWCSNPDSWS
                     NIVKLGVMKDKCVSCNRCIDVCPQNISSLFDRAQINNKCDLCGECIKVCLKDAICIMT
                     EEMSVEEIVEEVEKDFIFFFESNGGITFSGGEPTLQIDFLRELVDIFYDKGINIAIET
                     CGYFDWNKVNDVFEKIDHIFVDIKSMDDNIHKEYTGVSNKIILDNICRLSKLNKSMVI
                     RVPIIYGVNDSEENIRNTALFVKQNVPGGKMELLPYHKFGIDKYKALGLEDYIYEFDE
                     ICNNHMLKLKEIVELTGVKIIEYK"
     gene            13470..14021
                     /locus_tag="ACGI59_002738"
     CDS             13470..14021
                     /locus_tag="ACGI59_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFG9740518.1"
                     /translation="MDNKFPNVGENLRLLRQEMGISLDKASKMTGVSKAMLGQIERGE
                     SSPTVSTLWKISSGFKINFTTLLNENTNTYEVIKKEEVEPIVEQKGNMKLYPIYPFSP
                     QRRFELFIIELEENCTHVSSTHSHVLEEYVLVIEGKLDLNVDGKTYILESGHSIRFDG
                     TLEHIYKNLNKGKTIFHNIEVYR"
     gene            14185..15057
                     /locus_tag="ACGI59_002739"
     CDS             14185..15057
                     /locus_tag="ACGI59_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFG9740519.1"
                     /translation="MIIKVSMTVLVLILLYILYKYETQHIETTEYTIFNKKIPKEFDN
                     FKIVQVSDLHNKVFDKNNKVLIDKIESLNPDVIFITGDLVDGENKNFQVALDLIDNLV
                     EKYEVYHIIGNHEQKSLIKKYKHLYKDYFKELYSKKIINMENESVRIYRGNSHLNLYG
                     LIIPLECYPYLFANSKKLELSDNFVEDNLGKVNENEYNILLAHTPFFFEKYAKWGADL
                     VLAGHVHGGIIRIPFVGGVLSPNREFFPKYDWGKYEKDNSTMILNKGLGGSKVLIRLN
                     CKPEIVKITLKYEK"
     gene            15221..16201
                     /locus_tag="ACGI59_002740"
     CDS             15221..16201
                     /locus_tag="ACGI59_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9740520.1"
                     /translation="MGDTSRFYESVKNKYPKLKDGTRVHLWPKENIIIGPIYDEEDGY
                     KKSIGNVDSLSWKQIVILAECDGKNTTDDIINILSNRYRNIHNMKEKVMEFFMFYENV
                     YLTFEDDIQTLSSVFEITGNKSYITPLYFTIEIDGDNNTNDYFSEITSLLNCMYEKGC
                     RFIEVIGEDILKNKSMREIFQYMLDHFDLIVVTKDSFTIDRSLIKELDNYRHKVIWKV
                     YSKNDDTRIKLKEDIKITSLIKRGHTVVRGDREKAIKIEDMRFKDTKERFSKYGAEWS
                     HLYISSDGSVKSYSFQKDKRIYLGNIFNNSVEEIFLEMQNKIDKLEIVYQ"
BASE COUNT         6501 a         1616 c         2941 g         5418 t
ORIGIN      
        1 aataaagata taagaaattt atcaatttta ttttgagtta taaaatatta aagaaattgt
       61 aggaggacaa tatatgaaaa aaatattagt agcctgtgga gcaggaatcg caacatcaac
      121 aatagtttgt gacagagtag aaagattggt taaagaaaat aatatacaag cagaagttga
      181 gcaatgtaaa attgctgaat gttcaacaaa acaagaaggt gctgatttaa tcgtatctac
      241 tactatacta ccaacaactt atgatatacc tacaataaag gcaacaggat atataacagg
      301 tataaataca acagcattag ataaaaagat attaaatgca ttgaagtagt tttgtattta
      361 taaaaaaaat ttagtattaa atatagagtt gtatctttat aaaattttaa atatagagat
      421 atagctcttt ttatgttgtt tcaagtatac aactatatga tataaatctt caattttatg
      481 aaaaagattg ttattacttt gtaagtttat aaaagattta aagttattgt tatgttatac
      541 taatttatgg gtatttttta gaagtactaa taaaggaggg atttaatact tatgataaaa
      601 caatttgtca aatattataa gccatataag aaaattttta cactagacct tatagcagct
      661 tttctatttt ccttatgtga tttagtttat cctatgataa ctagaaatat aatggatgac
      721 gttgttccaa ataaaaactt aagaatgtta gtagtttttg cagtagcact tatacttata
      781 tttatagcaa aagcagggct taactatttt atgcagtatt ggggacatgt tattggtgta
      841 gatatgcaag ctgatatgag aaatgaggta tttacacact tacaaagact tccaaatacg
      901 tattttgata ataacaaatc tggagtaact atgtctagaa tagtaaatga tttaatggat
      961 ataacagaac ttgcacatca tgggcctgaa gatttattca tttctatagt aatgttggtt
     1021 ggttcattct ttatacttat agatataaat attcctctta cgcttataat atttgctata
     1081 ttaccgttta taatttggtt tgcaatagct aaaaaagata agatgaatat tgcttttatg
     1141 aagagtagag ttactatagg tgatgtaaat gctactttag aaaatagtat agcaggtatg
     1201 aaggtaacta agtctttttg tactgagaaa gaagagttaa ataagtttgt aagaagcaat
     1261 aaattattta gaagagcaag acaagattct tataaggtaa tggcagaata ttactctggt
     1321 atgaatttat acatggatat cttagagtgg gtagttgtaa tagctggtgg ttattttact
     1381 tatataggaa agattacttt gggtgatttt gctgcttata tactatatgt aaaaatgttt
     1441 atacaaccta tgaaaaaatt aataaacttt acagagcaat atcaaaatgg tatgacaggt
     1501 tttaaaagat tcatagagat aatggaacaa gaccatcaaa aagaagctaa aaatcctatt
     1561 gaacttgaaa atgtaaaagg tgacattgag atagaaaata tatctttcac atatgaagat
     1621 aagactcaag ttttggataa tttaagttta tctataaaag ctggtaagac tatagcatta
     1681 gttggacctt ctggaggagg aaagactaca ttatgtaatc tattgccaag attttatgaa
     1741 tttgataagg gtgatataaa aattgatgga aagagtataa aagatgttag tttaaaatca
     1801 ttgagaaaaa atataggtat agttcaacaa gatgtatttt tatttacagg aactataaga
     1861 gataatattc tttgtgggaa ccctaatgct acagatgaag aaatgatagc tgctgctaaa
     1921 aaagctagaa tacatgactt tgtagagact ttgccagatg gatatgatac ttatatagga
     1981 gaaagaggcg taaaattatc aggtgggcaa aaacaaagaa tttcaatttc aagaatcttt
     2041 ttaaagaatc ctccaataat aatattggat gaggctacat ctgctttaga caatgtaaca
     2101 gaaagagaaa ttcaagagtc tttagaggaa ttaagtaaag atagaacaaa tttagttgtt
     2161 gcacatagat tgactactat aaaaaatgct gatgaaatca tagtattgac ggataaagga
     2221 attgaagaaa gaggtactca tgaagagcta gtaaacaaaa atggagtata tagtaggtta
     2281 cacaataact agtatacaaa ttttacacca tagctaacat acacattaaa cttacaaaca
     2341 taggatatat taagattcat aaagacaatt cattttatat ttataagatg ttttatgaat
     2401 aaaatgtatc ctatgtttta ttttttgcaa attacattta attctctttg taagaataga
     2461 gttaaatagc caatggtcgg gataacgttt ttacaaaaag aataatttag aaaactaact
     2521 ttaatttatg gaatttatcg aattttatga aaaaaatcga attttgactt gaaatgtttt
     2581 tcaaaagtag taaaataatg gataactata catacatttt ataaaaatat atttattatg
     2641 aggggggatt ttgtatggag gcaataacag ctttttgtct agttttagta tcactgacga
     2701 taggagatat agtttcagca aaaactaaag cttttgttcc gtcggttttt gtatcagcaa
     2761 ttatatttat agtaggattt tggacttttt tcccagaaaa tattgtggac ttagccgctt
     2821 taggaacacc attagctcaa ttaggtatgc ttttgttaat aactcatatg ggaactatga
     2881 tgagtattaa ggaattagca ggtcaatgga aaacaatagt aatagccttg gcaggaatag
     2941 taggaatatg tgtaggtgct ttggcattag ggacagtagt atttggatgg gatacagcag
     3001 taatagctac acctcctctt actggagggc tagttgcatc tataatgatg gcagatgctg
     3061 ctacagcaaa aggtcttgca agtttatcag tacttgcaat tttgatgtat gttgcacaag
     3121 gatttgcggg ttatcctatt acagcattaa tgcttaaaaa agaaggaaaa agacttttat
     3181 cagactttag aagtggaaaa gtaactgtaa atacagatga agaaaaagtt aaggatttac
     3241 ctgaacaaaa aagcagattt aagataatac cagacttacc agaaaaatat gatacgacat
     3301 atatgataat acttagatta ggatttgtag catggttagc agtacaattt acaaatctta
     3361 caaatgaagt tgtatcaaga tatgttatat gtttaatatt tggtgtaata gcatcagaac
     3421 ttggaatagt tgatagaaaa ccacttaata aatctggttc atttggatgg ttaatgacat
     3481 cattgatggc ttttatattt gcagggttag caaaagctac accagcaatg cttgttgaaa
     3541 tggcagttcc tcttgtagga atcataatat ttggtgtaat aggtatggga attttctcta
     3601 ttatagtagg taagctttta ggtgaaagta aagaaatggc tttttcagtt tcgcttacat
     3661 cactttatgg ttttccacca aactatatat taactgaaga ggcagtaaag gctttggctg
     3721 aaacaccaga agaaaaagaa tttttaatgg ataaaatgct tccaaagatg cttgttggag
     3781 gatttactac agttacaata gcttctgtag taatagcagg tatctttgta aatctaatat
     3841 agtatttaat tgataaaaag tattttaaat aagaagaatg tgtactactt ggaggtatag
     3901 aatggatatt aaagaaataa caaagagtta taaagactat gtaattaagc ttagaaggga
     3961 atttcatgaa aatcctgaga aaagtatgga agaagttaga acatcaaaga gagttaaaga
     4021 agaacttgat aaaataggga taccatatgt atcagctgga ggtacaggtg taattgcaac
     4081 tataaaagga gataatccag gaaagactgt agcacttagg ggtgatatgg atgctctaca
     4141 agttgttgaa tgcactgatg tagaatacaa atctaaaaat gaaggactta tgcatgcttg
     4201 tggtcatgat ggtcacacat caatgcttct aggggcagca aaagtgttaa atgatattaa
     4261 ggatagtatc aatggaactg taaaattatt ttttcagcca ggagaagaag ttggtaaagg
     4321 tgcaagagct atgattcaag atggtgctat ggaaggtgta gatagtgtgt ttggaataca
     4381 tctttggact gatgtagaat ctgggaccat atcggtagaa gaaggaccaa gaatggcttc
     4441 tgctgatttc tttaaaatta ctgtaaaagg tagaggtggt catggttcac ttcctcatca
     4501 aggtgttgat gcagtgcttg caagttcagc aatagttatg aatctacaat ctatggttag
     4561 tagagaagta agtcctctgg aaccattagt tgtgagtgtt ggagttttaa attctggaac
     4621 tagatttaat gtaattgcga gtgaagctat cttagaaggt acaataaggc tatttaatcc
     4681 agagcttaga aagcaaattc ctggaatttt agaacgtata gcaaaatcta cagcagaagc
     4741 atatagagca gatgctgaat tagagtatgg gtacttaaca ccagcagtta taaatgacaa
     4801 agaatgttca aagatagcta cagatgcagc aattaagcta tttggagaag actgtataac
     4861 actatttgaa aaagtaacag gagcagaaga tttagctgag tttatgaata tagcacctgg
     4921 tgcactagca tttgtaggag ctagaaatga gtcaaaaggt gcttgttatc ctcatcatca
     4981 tggatgcttt aatatagatg aagatgctct agaaattgga actgctttat atgtgcaata
     5041 tgcagtggat ttcttaaata aataatttta atatactata aagaaaaagc agagactaag
     5101 tttatatctc tgctttttta ttgtgtatat tttttataaa tattgactat ataaaatata
     5161 tatgatagca taaagaaaat gataatcaat atcaattaaa aagaaggtga tttatttgaa
     5221 taatgtaata tttgcatttt tgataacatt attggctggt ttatctactg gtataggaag
     5281 ttgtatagca ttttttgcta aaaaaacaaa tactaagttt ctttctataa gcttaggatt
     5341 ttctgctggt gttatgattt atgtttctat gatagaaatt tttcctaaag cacaagatgc
     5401 tttgacaaaa tctatgggag agaaattagg aagttggtca actgtaatag ctttttttat
     5461 aggtatggct atcattgctg ttattgataa gctaattcca caagaagaaa atccacatga
     5521 aataaagaaa atggagaata tagaagaaaa gaatatcaaa aaaaataagt ctcttttaag
     5581 aacaggtata tttacagcaa tggctatagc aattcacaat tttcctgaag gattagctac
     5641 atttatatca gctcttgatg atgttacaat agctattccg atagcaattg caattgcaat
     5701 acacaatata ccagaaggta tatctgtatc tgtaccagtt tattatgcca caggagataa
     5761 aaagaaagct ttttattatt cctttttatc tggtatgtct gagcctttag gagcgattat
     5821 tggatatgta cttcttagaa attttttaaa tgatataact cttggaattg tatttgcaat
     5881 tgtaggagga attatggtat tcatatcact agatgaatta ttgccatcag ctagagagta
     5941 cggggaacat catttatcta tttatggatt gattgcagga atgggtgtta tggctattag
     6001 tttattacta tttaaataac ttttaaatat atgattgtaa cctaaaatta gtatcaaaat
     6061 gatataatta tttaaataga tttttaagct atttaaaaat atcattttgg agaatgagtg
     6121 ctatggaaaa gaaaataaaa ttcaaatttg actggtatag ttcacctgat aagatagaga
     6181 aatggttaga agatattgaa gctaaaggat ataatttata taagataaat aaatttggag
     6241 gaatatttta ttttcataag ggaagtccaa gaaaaataaa atacactgtt gattactact
     6301 atgatgtaga gaataattat tttgatgaac ataaatcaaa tggctggaat cttgtattta
     6361 ggagtattgg tagttttatg ggtacatctg catggtatat atggtcgaaa gaatacaaag
     6421 acatacctcc taaattatat agtgattctg ataaaacaaa taaagtaaag tcaattttat
     6481 ttatgaatat gtttatattc tttatgttag tagtaatatt tatatattta ttgaatattg
     6541 aaataagaaa tttatatagt ggttatggag catctgtaac tgctattgta ctacagatta
     6601 tattgttgat tttatatgca gtacaattat ataggagatt aatgtattat ttaaggctta
     6661 aaaagtttaa ataaataaaa gaaggtgtcc taaaattatt tttttaatat tttaagacat
     6721 cttcttttgt cagtaagtac tgtttttaat tatagtaatt tctgtcttta aattgaaata
     6781 tcaaataata gttgtatggg attgagtatt gataaaaata atttaaaaat gttatcctaa
     6841 agtatcagat atacaaaatt atctttaaat atattataaa tattttaatt gtattaacta
     6901 gaaattatgt aaggaggcat tttatgatta agatagcagt ttgtgaagat gaaaaagaaa
     6961 cacaactttt aatagaagat taccttgaga atatattaaa agatataagt atagaatatg
     7021 agatacaaaa gtatatatct ggagaagagt tactggaaag taatttaaaa gatatagata
     7081 tattacttct tgatataaaa atggaaaaat taaatggaat ggatactgca agaaagatta
     7141 gagaagtaga caatgaaatg gaaataatat ttgtaacttc attaatagat tatgtgcaag
     7201 aaggttatga ggtaagagct tataggtatt tactaaagcc aatagagttg gaagaactaa
     7261 aaaaacatgt gctaacttgt atcaaagata ttgaaataaa taaggaaagt catattacaa
     7321 taaaaaataa gtctaataca tataagattt atttaaatga aataaaatat atagaagttc
     7381 aaaaaaaaga tatgctaata cacacaataa acaagaattt tgatataaaa tatagtttag
     7441 gcaaaataga aaaagaatta aatccatata aatttataag atgccataaa agttttatag
     7501 taaatttaag atatgttgaa aatataaagc ctaatactgc aatactggaa agtggagaag
     7561 aggtacctat tagtagatat agatataaag aagttaagga gaaattctta aaatttcttg
     7621 gtgatacaat atgctaaact ttatgactga gctattagta gttttatcta tatttataca
     7681 gttttctata atgaacggaa ttttaattta taaaaaggat agtaaaaaaa atgaaaaaat
     7741 agtattctat attgtcttgt ttcttattta tattataatt cagataagta gattatttaa
     7801 tggaggaaat tactttagta atataaacta tttattttat atattagcat ttattgcttg
     7861 gggaggatac tataattatt tttataagat gggttatatt aagtattcca ttttctttta
     7921 tctatttagt gtatattata aatgtttgga tgctgttata tatagaatac tattttttgc
     7981 aattacaggt aatgttattt catctaaaga atctttatta gtaccttact tagaatctat
     8041 aaaaattcag atatatagta atttaataat tattcttata tatcttatat ttagcttttt
     8101 tagaaagtct ataaaattaa ttagccaaga taagagaaat tatgtatatt tattatttgc
     8161 cttattagtt aatacaatga atatgcttgt aaattatgta ttaaaaaaat tagaacggtt
     8221 tgggtcatta cattcagaag gatactattt tgataatttt gttaatccaa aattggtagg
     8281 agctagtagt atatttctta tactattgtt taaagaaata ataaaagaga atagattaaa
     8341 atctcaagct gaattaataa aaaataagtt agatatgcaa tatgcgcatt atttaagtat
     8401 tcaagaatct catatgcgag ttaagaaact atatcacgat ataaataatc acatttattg
     8461 tatagacaac cttagaaaca atagcaaaga aattaatgaa tatgttaaca atttaaaaga
     8521 tgaaataaaa acatttaaat atatctataa tacaggaaat atgatattag acattataat
     8581 caacgaaaag agtgaagtat gtttaaaaaa aggaattaaa tttacttgct ctataaattt
     8641 ttcaaaagta aattttgtaa aacctataga tgtgtcaagc atattttcta atattttgga
     8701 taatgcaata gaagcatgtg ataaaatagt ggatgaaaat attaataaat atattagaat
     8761 taaaggaact ataacaaggt cattttttgt acttaaatgt gaaaatagta aactaaatca
     8821 gcttactttt aataaaaata tattgttaac aaacaaaatg gataagttcg tacatggaat
     8881 aggtattcag agtattaaat cttctttaca aaaatataat ggagaattat tatttgaaaa
     8941 tagtatcgat aaatttatac taaatatata tataccattg gaccaagata ctgacagttg
     9001 gggcgattaa gttaccagtt gggacatatg tattgataca aaaaaataag gatgttatct
     9061 tttaataaca aaagataaca tccttatttc tgtattaagg agcttataat tttactaata
     9121 gtaagttata agaattggat aggaaggtga tatgtattga ataataaatc tttaatataa
     9181 agaaaatata aatatattct agtgcaatga ataatacttt agagcggtta gtgtgatggg
     9241 aggattgtgt atgaaagtaa aaaggaatat aaatagagtg gcaataattg tggctttaaa
     9301 tcttagctta gcattgatgg tagggtgtat gtctcctgta tctgataaga acttaagtag
     9361 ctcagaacaa attagtaaag attcattgaa agaacaagtt gaagataaaa aagatgaatc
     9421 tattgagcta tttaataatt actcacaaaa gattaatgat gcgaaaggta gagaagaagt
     9481 aaaaaaagat ttatctcaaa gattttcgaa agctataata gacagtggtt atagtattac
     9541 taactcaaaa tcagatggta gctttgtagt atctataaat ggtgctgaaa aaccacctga
     9601 aaaaaatatt cgaagtttat tctattctat gtctgaagac gataaagaag gaaaagtaac
     9661 aatagatatt ctctgtgcta aagagtatcc agaagatgat aagttatctg agagtgataa
     9721 atatgttaaa tttatataca atttatttaa atcattgact gatacgaact taactgagaa
     9781 agaaattttt tctatggttg aagaagactt taataaggga gtaggtgttg tagagcttcc
     9841 ttatatgaag gatatacatg ttgaagttaa caaagttaaa caatcaacta aggttttaaa
     9901 actaagtcta gtatacaaat ttgatatttc aaatcattag tatattagac ataactataa
     9961 caatatagaa aatatatcat agaaaatttt tttagatttt ataaatttaa caaaaaaagt
    10021 attaatatat aggtgttcaa ggtagatgaa aaaaaataag ttattttgct tccattttat
    10081 gctaaaggac agtataatat aattaaagta tgttataaca tacaaaaatg tagtatatat
    10141 tatccaaagg gggattttaa atgagtcaaa ctaatagaat agaagcattt agacaagaat
    10201 atattaattc taaacctatg atatgttgtg aaagggctag aatttttaca gaatcacata
    10261 aaaaaactga gggagaagct atctgtataa gaagagctaa agcttttttg gagacatgta
    10321 aagaacttcc tataaaaatc tttgaaaatg aattaatagt aggtacagca ggaaaattta
    10381 gaagaacagg gatattgaca ccagagtttt cttggcaatg ggtagacaaa gaaatggata
    10441 cttttgataa aagaactcaa gacccatatg taatatcaaa agaacaaata gaatttataa
    10501 gaaaagaaat atttccatat tggaagggaa aatctttgga agaagttttc ttagcaagaa
    10561 tcccagaaga tacagctaag atattagtag atacaggaat aatagataat gattctaagt
    10621 ggagacaggc agttggtgaa gtaacaccag attatcaaga tatattgttt gtaaaaggat
    10681 ataaagggat taaggaagat gcagataaaa aaattaaaga attagatata tcagtttcag
    10741 aaaatattga aaagatagat ttttacaaat ctgtttctat agttgcacag ggtattatga
    10801 cattggcaca aagatattca aatcttgcaa aagagatgag taagcaagaa acagatgaaa
    10861 agagaaagtt agaacttata aaaatttcag agatatgtat gaatgtacca gcaaaccctc
    10921 caactaattt ttatgaagca atacaatttg tatggtttgt tcaattaggt ggtattctat
    10981 cagaaaatcc attggcatta aatttaggta gatttgatca atatatgtat ccatattatg
    11041 aaaatgatgc tagagaaggt aaaataactg aatcagaagc tcaagaattg atagaagcac
    11101 tttggattaa gttatcagaa tgggtatgga ctatatcagc aaatacagca aattactttg
    11161 caggatataa tcaattccaa aatcttactg ttggtggtaa aaagagaaat ggtactgatg
    11221 gaacgaatga tatctcatat atgtgtttaa aggctacaga aagtgtaaaa actcaccaac
    11281 caggattaag tgttagagtg agtcaaggag caccagacaa tttcgtaatg gcagttgcta
    11341 aattggtaaa acaaggaact ggtttccctg ctatacacag cgatagtgca ggggcacaaa
    11401 tgttattaca agatggatat gatgcagaag atgctagaga ttggagtaac tgtggttgtg
    11461 tagttcctca ttttagaaaa acaggacagt ggacttcagc cgtaaatatc aattttgcag
    11521 cagctttaga atatgctatg aatgaaggta agagtagatt aactggagaa aaaatggggt
    11581 tagatacaaa aaatatcaca gaatttactt cttttgaaga acttaaggat gagtttttaa
    11641 agcaattagc atatcttgta aaaagttctg ttataggaac tactgttgct caacaaattc
    11701 ataaagaaat ggtaccaaga ccatttttat ctacttgtgt agatggatgt ttggataagg
    11761 gagttgattt aagtaaagga ggagcaaaat ataatatagg tcctgtatta actggtatag
    11821 ggttaggtgt agtttcaaat tcattggcag ctataaaaaa attagtattt gaggataagg
    11881 tgactacgtt agaagaatta acaaaagcac taaataatga ttgggaaggt tatgaagaat
    11941 taagaaaact tgcgttggat gttcctaagt atggaaatga taatgattat gtagattcat
    12001 tagcaattga agtttctgat ttttattata ctgaaactag aaaatacaag gatatttttg
    12061 gctctaaatt taatagtgca tttatgggta tatcaaacta tgtaccaact ggaaaaatcg
    12121 taggcgcaac tccttgtgga agaaaagcga caaaaccttt gacagaggga gtttctccat
    12181 ttgttggtac tgatacaaca agtccactag cagcaatgaa atctgcatca aaaataaatc
    12241 atgatgttca tactggtgga acacttctaa atttaagact gaatcaagat ttggtggaaa
    12301 ctgaaagagg acttagaaat ctaacatcta tgattaaatc atattttgct ttaggaggat
    12361 tccatgtaca atttaatacg atatcaaatg atactctatt aaaagctcaa gaaaatccag
    12421 aagaatataa agatttatta gtgagagttg ctggatatag tactcaattt gttaatttat
    12481 caagagaaat gcaagatgct ataatagcta gaaattcaca tagcaatttt taattatgag
    12541 gtagattatg agtaaaaaag gtagagttgt aaaagtacaa catttttcag taaatgatgg
    12601 agatggaata aggactacta tatttttaga aggatgtaaa ttaaaatgta agtggtgttc
    12661 taatccagat tcctggagta acatagtaaa attaggtgtt atgaaagata aatgtgtatc
    12721 ctgtaataga tgtatagatg tttgtcctca aaatataagt tcactatttg atagagcgca
    12781 aataaataat aaatgtgatt tatgtggaga gtgtatcaag gtatgtctta aagatgccat
    12841 ttgtataatg accgaagaaa tgagtgttga agaaatagta gaggaagttg aaaaagattt
    12901 tatatttttc tttgaatcta atggagggat aactttttct ggaggtgaac ctacacttca
    12961 gattgatttt ttaagagaat tagttgatat tttttatgat aaaggcataa atattgcaat
    13021 tgaaacttgt ggatattttg attggaataa ggtaaatgat gtatttgaga aaatagacca
    13081 catatttgta gatataaaat ctatggatga taatattcat aaagaatata caggagtaag
    13141 taataaaatt atattagata atatatgtag gctttcaaag ttgaataaat ctatggttat
    13201 aagagtgcca attatttatg gagtaaatga tagtgaagaa aatataagaa atactgcatt
    13261 atttgtcaaa caaaatgttc caggaggcaa gatggagtta cttccatatc ataaatttgg
    13321 aatcgataaa tataaggcat tagggcttga agattatata tatgaatttg atgaaatttg
    13381 taacaatcat atgttaaaat taaaagagat agtagaactt acaggagtaa aaatcataga
    13441 atacaaatag ctatcaggag gattttaaca tggataataa atttcctaat gtgggagaaa
    13501 atttaaggtt attgagacaa gaaatgggca taagtttaga caaagcttca aagatgacag
    13561 gtgtcagtaa agcaatgctt ggacagattg agagaggtga atcaagtccg acagtatcta
    13621 ctttatggaa aatttcatct ggatttaaaa taaattttac tacactatta aatgaaaata
    13681 caaataccta tgaggttata aagaaagaag aagtagagcc tattgttgag caaaaaggga
    13741 acatgaagtt atatccaata taccctttta gccctcaaag aaggtttgaa ctttttataa
    13801 ttgagcttga ggaaaactgt actcatgttt catctacaca cagtcatgtt ttagaagagt
    13861 atgtactcgt aatagaaggt aaacttgacc ttaatgtgga tgggaaaact tatattttag
    13921 agtctggaca ttctattagg tttgatggaa ctttagagca tatatataaa aatttaaata
    13981 aaggaaaaac tatatttcac aatatagagg tttatagata attttatagt tatccttttc
    14041 taaaataaat gaaaatttta gaaaaggata acttattttt ataatgtcga aaagatttat
    14101 atgtatgtga tgatgttatt gttttaaaaa aaacataaaa gcattataat aatattaaca
    14161 aaatagaata aagaggaaga gaatatgatt ataaaggttt caatgacagt attagtatta
    14221 atattattgt atattctgta taagtatgaa actcagcata tagaaacgac agaatatact
    14281 attttcaaca aaaaaattcc aaaggaattt gataacttta aaatagttca agtgagtgat
    14341 ttacataata aggtgtttga taaaaataat aaggtattga tagataaaat tgaatcatta
    14401 aacccagatg ttatatttat aacaggagat ttagtagatg gagaaaataa aaattttcaa
    14461 gttgcattag atttaataga taatttggtt gaaaaatatg aagtatatca tataattgga
    14521 aatcatgaac aaaaatcttt aataaaaaag tataaacatc tatataagga ttattttaag
    14581 gaactatatt caaagaaaat aataaatatg gaaaatgaat ctgtaagaat atatagaggt
    14641 aatagccatc ttaatttata tggtttaatt ataccattgg aatgctatcc atatttattt
    14701 gctaatagca aaaaattaga actaagtgac aattttgtag aagacaatct aggtaaagtt
    14761 aatgaaaatg aatataacat tcttttagca catacaccat ttttctttga aaaatatgca
    14821 aaatggggag cagatttggt tttagcagga catgttcatg gaggtataat aagaatacct
    14881 tttgttggag gtgtattatc tccaaataga gagttctttc ctaagtatga ctggggaaaa
    14941 tatgaaaaag ataatagcac tatgatttta aataaaggct taggaggctc aaaagtttta
    15001 attaggctta attgtaaacc agagatagtt aagattacat tgaagtatga gaaataagat
    15061 tttatataac atatataaac atcaataaca tataaataag gttgatgtta aatccattta
    15121 aacatatata atgaccaatt ttgaataatt gttgaattaa taaaaataaa gtgctatttt
    15181 tagcatagaa tatttaaact atggggggta gtaaagaatc atgggtgata caagtagatt
    15241 ttatgagtct gtaaaaaata aatatccgaa attaaaagat ggaactaggg ttcatctgtg
    15301 gccaaaagaa aacattataa ttggtcctat ttatgatgaa gaagatggat ataaaaaaag
    15361 tatcggaaat gtggatagct taagttggaa gcaaattgtt atactggctg aatgtgatgg
    15421 gaaaaatact actgacgata taataaatat attatccaat agatatagaa atatccataa
    15481 tatgaaagaa aaagttatgg aattttttat gttttatgag aatgtatact taacgtttga
    15541 agatgatata cagacactat caagtgtatt tgagataaca ggaaataaat catacattac
    15601 tcctttgtac tttactatag aaatagatgg agataataat acaaatgatt atttttctga
    15661 aatcacaagt ttattgaact gcatgtatga gaaaggctgt agatttatag aggttattgg
    15721 tgaggatatt ttaaaaaata aaagtatgag agaaatattt caatatatgt tagaccattt
    15781 tgatttaatt gtggtaacta aagatagttt tactattgat agaagcttaa taaaggaatt
    15841 agataattat cgtcataaag tgatatggaa agtatattca aaaaatgatg atacacgtat
    15901 taaattaaaa gaagatataa aaataaccag tttaataaaa aggggtcaca ctgtagtaag
    15961 aggagacaga gaaaaagcaa taaaaataga agatatgagg tttaaagata caaaggagag
    16021 gttttcaaaa tatggagcag agtggagtca tttatatatt tcttctgatg gtagtgtaaa
    16081 atcttattca tttcaaaaag ataaaagaat ctatttaggc aatatattta acaattctgt
    16141 tgaagaaatt ttcttagaaa tgcaaaataa aattgacaaa ctagaaattg tgtatcaata
    16201 gggggaaagt ttaagattta tagaagattt atagattgtt tatttaagtt tgtgtcaata
    16261 attataatga ggtgagatta cttgttaaaa ttattggaca aattttttta tgatgttcta
    16321 cttaaatttt ggctgtttat gatgctgtgg atttttagag aactttttac aaatttcaaa
    16381 atgactaaaa aatttaaatt tcataaaata tttatgtaaa aatcatataa aattcatata
    16441 gcatatgtat catatgaggt gtagaaagac tagctt
//
LOCUS       DAYAPA010000093        16351 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.093, whole genome shotgun sequence.
ACCESSION   DAYAPA010000093 DAYAPA010000000
VERSION     DAYAPA010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16351
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.093"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            94..672
                     /locus_tag="ACGI59_002741"
     CDS             94..672
                     /locus_tag="ACGI59_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator ArgB-like protein"
                     /protein_id="HFG9740521.1"
                     /translation="MFKRYAEKMTSVLICNNMIDNNESKVYSYGFEILIAFIVNITTM
                     LFIGFLFGKFTYVLFFLMCYCPIRQFSGGYHADNYFRCLLTFIFIILSTILIIENINI
                     DLFKNIIMIIASVSWVGICVLCPIEHRSNPISDREKLVYKKTAIFISTVVLLITISSL
                     SLSIFVDYFTYSAFAMFWIFVMLVLGKLKAKV"
     gene            702..848
                     /locus_tag="ACGI59_002742"
     CDS             702..848
                     /locus_tag="ACGI59_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="HFG9740522.1"
                     /translation="MKKFIVRFMKFASSLALSTAILSANSTCPWIIHQPKVPKEISNL
                     KKTN"
     gene            1144..2523
                     /gene="scfB"
                     /locus_tag="ACGI59_002743"
     CDS             1144..2523
                     /gene="scfB"
                     /locus_tag="ACGI59_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0031179 - peptide modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioether cross-link-forming SCIFF peptide
                     maturase"
                     /protein_id="HFG9740523.1"
                     /translation="MSMIHKFSMNGYNIVLDVNGGAVHVLDDVAYDLLDFYKEKSKEE
                     ILEILKSKYQEEKINEAYEEILNLEKEGLLYTEDTYQYHPSFVHREPVVKALCLNVAH
                     DCNLKCKYCFAAQGDFGGEKELMSFEVGKAAIDYLIANSGSRKNLEIDFFGGEPLMNF
                     EVVKQLVDYGRSVEKDYNKNIRFTITTNGVLLNDEIIDYINENMHNVVLSLDGRKEVN
                     DNMRPTLNDKGSYDITLPRFKKLVEKRAKDKYYYIRGTFTRDNLDFSKDVMHFADLGF
                     KLTSVEPVVGDESNPYALREEDLPKIFEEYEKFAVEYADRQLQGDGFKFFHFMIDLNQ
                     GPCVIKRITGCGAGNEYLSVTPNGDIYPCHQFVGNEEFKMANIFDEEIVLPENLKNMF
                     REAHVYTKEECKQCWNKFYCSGGCHANAINFNNDISKPYELGCEMQRKRTECSIMIQA
                     KLMLEGATN"
     gene            2552..3049
                     /locus_tag="ACGI59_002744"
     CDS             2552..3049
                     /locus_tag="ACGI59_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFG9740524.1"
                     /translation="MIRDYLEDKPLIDESVFVAKSADVIGNVKIGKDSSIWYNAVVRG
                     DEGPITIGENTNIQDCSIVHGDTETIIGNNVTVGHRSIVHGCKISDNVLIGMGSIILD
                     NAEIGEYTLIGAGTLITSNKKFPPGVLIMGSPGKVVRELTEEDKKYIDESYEWYLEAA
                     QNQKY"
     gene            3347..5011
                     /locus_tag="ACGI59_002745"
     CDS             3347..5011
                     /locus_tag="ACGI59_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC1 kinase family protein"
                     /protein_id="HFG9740525.1"
                     /translation="MRISYRNLKRYREIGYVLIKYGFSFIVERLNIEGIAYKIPLFDP
                     PEEIKNMTTGERMKRVLEELGPTYIKIGQILSTRKDLLDQDIIDEISKLRDDVEKFDS
                     NIAIDIFKEEVGLSIEEIFLEFKEEAIAAASIGQVYEGVLKTGEEVIVKIQRPNIEKI
                     IKSDLEILRTIANTLKDLKKDFNLDLVQMIEEFQTQLMRELDYTFEAINATKFSRIFK
                     NSDEVYIPKVYSEYNTKKILVMEKVNGTKLSDVEKIRRLGYNTKTIVEIGVRSFFTQV
                     LSHGFFHADPHPGNIFVVAKNKIAYIDFGMIGIIDNKTLNQLNEIALAGVEKNVDKII
                     YLLIEMDALNGEADIKGLRQDLLYLIHYYYDISIEKINVTDILNELFRFFRQYKIVMP
                     AQFVTLAKTVITLEGTSRTLNTDFSFGSMGKEFMKHHYKSKFNPKNVVLSSRQNVEEI
                     LLDIKTIPKQLKAILKNIERNNIKMQIEDVKMTRLENCIIELTSQISLSLVLASIIVG
                     SSLIIASPNIENNIWIKFTAIAGFFISFIIGLCLVIRSIRSKYKKD"
     gene            5024..7474
                     /gene="recJ"
                     /locus_tag="ACGI59_002746"
     CDS             5024..7474
                     /gene="recJ"
                     /locus_tag="ACGI59_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFG9740526.1"
                     /translation="MKKWILKYKGKIEESEFSKKLNISPEICQILKNRGICTEKDSEI
                     FMNPSLDYLRDPFLMKDMQKAVDRIKLAVEKNEKIWIYGDYDVDGVSSTSILCLYFKS
                     IGYNVDYYIPNRLEEGYGINEDAIKLISSRGCDLIISVDCGITSVSEVNTANDLGIDV
                     IITDHHECQSEIPSAFAVINPKQEDCNYPFDSLCGCGVAFKMIQALTPKEEFKTSMYN
                     YLEIVTLATICDIVPLIDENRIIVKNGLKSMKEGKNIGLRELIKVCGVESDKIGSSHI
                     GFAIGPRINASGRLGYSYLGVELFTTQSQEEAVEIASILEEKNNERQMIEAKMYHEAE
                     EMLKSNSRYNDDKVLVLAKEGWQHGIIGIVASKLTEKYYKPTILLGIENGEATGSARS
                     IKGFNIFEALIKCKDLMTKFGGHEQAAGLSLDSDNVEILANEINKFADYNLTEDDMIE
                     NVNVEFELQENVINLNLVEELHKLEPFGLNNPNPRFIVRNYILKDLKVIGKNQQHLKL
                     SIEKEKSYECIGFNMSHLKSMYKVGDKVDVLFQLDENNYMGNRKVQFLLKDIRLARPK
                     SASNDKLSLKLMSKIIPKDTQSLYNISVSDFELFDGNTDINIFDYFEKDTLIISNSIN
                     GFYRAMSDISLIDLDFNINYNIIEDVSKNTDKLELIFSPNIDKIDLKRYNNIILYDYL
                     YNKGEYSYIYENKREESEIIKYYNKTDLLYLKNVVSNIVPSRDEFITIYKQALIKKEI
                     DLDMVNIRETFNVIPLKFFTILNVFRELNLLDFNLNYEKNSVLIRILPKPQKKLDLNE
                     SLILNNLKNLEKQYNSSY"
     gene            7487..7999
                     /locus_tag="ACGI59_002747"
     CDS             7487..7999
                     /locus_tag="ACGI59_002747"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFG9740527.1"
                     /translation="MDLKNFIRNIDDFPKPGIDFKDVTTLFKDGDAFKYAVDSIVEEL
                     KDKDVDLVIGPEARGFLMGTPVAYALGVGFVPIRKPGKLPGEVESYEYGLEYGTDTLE
                     IHKDAIKKGQKVAIVDDLLATGGTMEAAAKLVEKLGGEVVSMQFLIELKFLNGREKLS
                     NYDVNSLIKY"
     gene            8073..10280
                     /locus_tag="ACGI59_002748"
     CDS             8073..10280
                     /locus_tag="ACGI59_002748"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFG9740528.1"
                     /translation="MHDKELQEIIEKIKVYAPNANFEMVEKAYNLAKKAHEGQYRKSG
                     EPYFIHPLAVANILIEMELDIETITAGLLHDVVEDTEYTYEDIKKEFGKEVADLVDGV
                     TKLGQIKYRSKEETQSENLRKMFLAMAKDIRVILIKLADRLHNMRTLKYMPPEKAKYK
                     AKETLEIYGGIAHRLGISKIKWELEDRALRFMDPEGYYDLVSRVSMKRSQREDYIQGI
                     VELLKDKFKDLSIPCDVYGRPKHFYSIYRKMQKKHKTFEEIFDLTAVRILVDNVKDCY
                     AVLGIVHTLWRPIPGRFKDYIAMPKPNMYQSLHTTVVGPDGEPLEIQIRTHEMHNIAE
                     NGIAAHWKYKEGISSKESKVEEKLQWLRQMMEWEKDLKDPQEFMDALKEDVFNSQVYV
                     FTPKGDVIELPAESTPIDFAYRVHTNVGNKCVGAKIDGRIVPIDYKLQNGNIVEVLTS
                     SNSNGPSRDWLNIVKTPNAKSRIRQFFKKERREENVDRGNTILEREFKKHDIPQKDPL
                     VEKYMALVAKKFNQPSVEDLIATVGYGGIMSSQVVSKVKDLYLKEVKKAKKEQQIQEE
                     DLNKHNISETEYSKKRKKNASQGIIVKGLDNILVRFAKCCNPLPGDEIVGYITKGRGV
                     AIHRADCPNANNNGDFFQNRLVEVSWSSAGKGKFEAEIQIKAIDRRGIVNEITHIVAM
                     DKLSLNGINARKGKDSIVSINLLVEVNDTEELKILMKKLKAIPGVESIYRMTN"
     gene            10305..10754
                     /gene="dtd"
                     /locus_tag="ACGI59_002749"
     CDS             10305..10754
                     /gene="dtd"
                     /locus_tag="ACGI59_002749"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFG9740529.1"
                     /translation="MRAVVQRVSSSKVTVDENTIGQINKGLLVLLGVTHDDKSSDVDY
                     MIDKILNLRIFEDENDKMNLSLMDIGGELLVVSQFTLYGDCRKGRRPGFSNAARPELA
                     NNLYEEFVKKAKDKGVTVGTGQFAAHMMVELTNDGPVTILLDSSKSF"
     gene            10769..11383
                     /locus_tag="ACGI59_002750"
     CDS             10769..11383
                     /locus_tag="ACGI59_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFG9740530.1"
                     /translation="MIIKKFVDSYFGVNTYVLGDEKTKKCAVIDPGGSLVETLSFVKE
                     NELNIEYIILTHGHGDHIGYVKDIKEKTGAKVVAHVDEKELLNDKNKNLSYTMRCGAQ
                     EFDADIYVNDKDKLELGELKMTFLHTPGHTQGCMCIRVEDEMFTGDTLFAGSIGRTDL
                     YSGDFNQMEKSLKKLCKYEDAVRVYPGHGPTSTLGVEKKTNPYM"
     gene            11474..13030
                     /locus_tag="ACGI59_002751"
     CDS             11474..13030
                     /locus_tag="ACGI59_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase"
                     /protein_id="HFG9740531.1"
                     /translation="MLYVFLKGHDYKYEVAELIKLFTSEFKFKDSNANNNIESKHLIN
                     RLIYENGVLFSSTEYYENGNLKYESIQNIENMNLEFLDGFDSSKTIEEFLNSLDSESK
                     RNFKKLCKENIKKSMFLVLKQVFNSYVPWGILTGIRPVKIVHNLMDKKLDDNSIRTIL
                     KDNYFIIDEKIDLALEIAKRERLFMYPIDKNKISLYVSIPFCPTRCVYCSFPSNSLKQ
                     FGHLKREYVYKLIEEIKGFAKVIKDTKKEVETLYIGGGTPTTLDEEELDLLINSLFNE
                     LDLSKIKEFTVEAGRPDTITEQKLRVLKKHNVSRISINPQTMNDDTLVKIGREHRVSD
                     LVDCFNMARRMGFDNINMDIILGLIDEDLNMVKNTLEEIKKLSPESLTVHTLAIKRAS
                     NLNINMDRYKEHLTQYEEMVKMIDLSMKYAKDMGLNPYYMYRQKHMLGNLENIGYAKE
                     GYECIYNIQIMEEKQSNYALGAGAISKFVYVDEDRIERVENVKNVEQYIERVDEMIKR
                     KKEEVYKNVD"
     gene            13020..14282
                     /gene="hisS"
                     /locus_tag="ACGI59_002752"
     CDS             13020..14282
                     /gene="hisS"
                     /locus_tag="ACGI59_002752"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFG9740532.1"
                     /translation="MLTKAPRGTKDITPKEAYKWRYVENKFREICALYGYEEMVTPIF
                     EHTELFKRSVGDTTDIVQKEMYSFKDKGDREITLKPEGTAGVVRAFIENKLYADTQPT
                     KLFYVTPCFRYERPQAGRQRQFHQFGIEALGSDTPSMDAEIIALAVQFFNEVGLNDLV
                     VSINSVGCPVCRKEYNALLKEYLDSKADILCDTCNERREKNPMRVIDCKNPTCKENIK
                     DIPFIADHLCDDCKSHFDKLQEYLKEMNINFVIDKTIVRGLDYYRKTAFEIISNDIGA
                     QSTVCGGGRYDGLVEQLGGPKGISGIGFGLGIERLLLTLEGNGIEIENPQSTDIFIVT
                     IGEEANTRSFKLLKDLRQNHISADKDHIERSVKAQFKYSDKINSKFTIVIGDDELKND
                     TATLKNMKTSEQTTVKLSTLVEELKQKL"
     gene            14356..16143
                     /gene="aspS"
                     /locus_tag="ACGI59_002753"
     CDS             14356..16143
                     /gene="aspS"
                     /locus_tag="ACGI59_002753"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFG9740533.1"
                     /translation="METLKGLKRTHYCGELREKNINEEVVLMGWVQKKRNLGGLVFVD
                     LRDTSGLCQIVFDTDVDKEAFEKAEKLGAEFVIAVKGKVCERQSKNPNMPTGDIEIFA
                     TELRLLNKSETPPIYIKDDDDVSEALRLKYRYLDLRKPSMQRNLKLRHKVMNITRNYL
                     SNNRFCEIETPFLIAPTPEGARDYLVPSRVNPGKFYALPQSPQLYKQLLMVSGMDRYF
                     QIVKCFRDEDLRADRQPEFTQIDCEMSFVEQEDVMSMIEGLLEAIFKEVLDVELALPL
                     PKMTYAEAMSKYGSDKPDTRFGYELTDISDVVCNCGFKVFADATQPGKSVRGINVKGK
                     ADDFTRKQISSLEEHAKTYRAKGLAWMKVGQEGVTSPIAKFFNEEEMNAILTRMNAEV
                     GDLLLFVADKNSIVFDALGQVRLEVANRLNLLDKNVYNLLWVTEFPVFEEDEETGTFS
                     AMHHPFTSPMDEDLDKLEEGDKSSLRAKAYDIVLNGYEIGGGSVRISNSDVQSRMFKA
                     LGFTEERANEKFGYLLEAFKYGTPPHAGLAFGLDRLVMLLAGTDNIREVIAFPKNQNA
                     VCPMTNAPTLAEDEQLEELSIKVDIKDNE"
BASE COUNT         6565 a         1659 c         3060 g         5067 t
ORIGIN      
        1 ttttacattt ttaacaacta gttttgtaaa gtatattgaa ttttctcaaa aaagaatgta
       61 ctctatatat gtaaaatttt tttggaggta aaaatgttta aaagatatgc tgaaaaaatg
      121 acatctgttt taatttgtaa taatatgata gataataatg aatctaaagt ctattcttat
      181 ggatttgaaa tattaatagc tttcatagtt aatataacca ccatgctgtt tataggtttt
      241 ttatttggta aatttacata tgtattattt tttttaatgt gttattgtcc aataagacaa
      301 ttttcaggag ggtatcatgc agacaattat tttagatgct tattaacttt tatttttata
      361 attttatcca caatcttaat tatagaaaat ataaatatag acttatttaa aaatattatt
      421 atgattattg caagtgttag ttgggttgga atttgtgttc tgtgcccaat agaacatagg
      481 agtaacccta taagtgatag ggaaaagttg gtttataaga aaactgctat atttatatct
      541 acagtagtgt tattgataac aatttcaagt ctttctttga gtatatttgt agattatttc
      601 acatatagcg catttgccat gttttggatt tttgtgatgt tagtattagg taagttaaaa
      661 gctaaagtat aagtaaaata tgtatgaggg agagttaagt tatgaaaaag tttattgtaa
      721 gatttatgaa gtttgctagc tcattggcac ttagcactgc tattttgtca gctaatagta
      781 cctgtccttg gattattcat cagcctaaag tacccaaaga aatcagcaat ctaaagaaga
      841 caaattaaat acaagcactt atttatacaa aattatcagg aaaaaagaaa ttattatatt
      901 ttaataagat taaaacaata taaaatcaga tacaaaattt attttatagg agcctaatac
      961 ctatttggta atatattagt gcgccaattt agggggatat agtgtattta atgaaatgac
     1021 ttacggagag taagtcattt ttgtattgta tgtattttat gtaaacttat gatataatga
     1081 agggtaaagt ttaattaaaa taataaaaaa ctttagtata gatagtaatt taggaggtag
     1141 ggtatgagta tgatacataa attttctatg aatggatata atatcgtttt agatgtgaat
     1201 ggtggagctg ttcatgtgtt agatgatgtt gcatacgatt tgttagattt ttataaagaa
     1261 aaaagtaaag aagaaatatt agagatatta aaatcaaaat atcaagaaga aaaaataaat
     1321 gaagcatatg aagaaatatt aaatttggaa aaagagggtc ttttatatac agaagataca
     1381 tatcaatatc atccaagctt tgtacataga gagcctgtag taaaagcttt atgtttaaat
     1441 gtagctcatg attgtaactt aaaatgtaaa tactgttttg cagctcaagg tgattttgga
     1501 ggagaaaaag aacttatgag ttttgaagtt ggtaaggcag caattgacta tttgattgct
     1561 aattcaggaa gtagaaagaa cctagagata gatttctttg gtggagagcc tttgatgaac
     1621 tttgaagttg taaaacaact ggttgactat ggaagaagtg tagaaaaaga ttataataaa
     1681 aatataagat ttacaataac tacaaatggt gtactgctaa atgatgagat tatagattat
     1741 ataaatgaaa atatgcataa tgtagtttta agtttagatg gaagaaaaga agttaatgat
     1801 aatatgagac caactttaaa tgataaagga agttatgata ttacattacc acgatttaag
     1861 aaacttgtgg aaaaaagagc aaaagataag tattattata taagaggtac ttttacaaga
     1921 gataatttag atttttcaaa agatgttatg cattttgcag atttaggatt taaattaacc
     1981 tctgtagagc cagtcgttgg agatgagagt aatccatatg cacttagaga agaagattta
     2041 cctaagatat ttgaagagta cgaaaaattt gctgtagagt atgcagatag acaattacaa
     2101 ggagatggat ttaaattttt ccattttatg atagacctaa atcaaggtcc atgtgtaata
     2161 aagagaataa ctggatgtgg agctggaaat gagtatcttt ctgtaactcc aaatggagat
     2221 atctatccat gtcatcaatt tgttggaaat gaagaattta aaatggcaaa tatatttgat
     2281 gaagaaatag tacttccaga aaatttaaaa aatatgttta gagaagctca tgtatatact
     2341 aaagaagagt gtaaacaatg ttggaataag ttttactgtt caggtggatg tcatgcaaat
     2401 gcgataaact ttaataatga catatcaaaa ccatatgaat taggatgtga aatgcaaaga
     2461 aaacgtacag agtgctctat aatgatacaa gctaagttaa tgttagaagg agcaaccaac
     2521 taaatttata ttttaaagga gttgtttata tatgattaga gattatttag aagataaacc
     2581 tttaattgac gaaagtgtat ttgtggcaaa atcagcagat gtaattggaa atgttaaaat
     2641 aggcaaagat tcaagtatat ggtacaatgc agtagttagg ggtgatgaag gaccaataac
     2701 tattggagaa aacactaata tacaagattg ttctatagtg catggagata cagaaactat
     2761 aattggtaac aatgtaacag ttgggcatag gtctatagtt catggttgta aaattagtga
     2821 taatgtgtta attggtatgg gttcaataat attggataat gctgaaatag gagaatatac
     2881 tctgatagga gcaggtacac taattacatc taataagaag tttccgccag gtgttttgat
     2941 aatgggttct cctggtaagg ttgttagaga gcttacagaa gaagataaaa aatatattga
     3001 tgaatcttat gaatggtatt tagaggcagc acaaaatcaa aaatattagt ttaaagagtg
     3061 gttgtttcaa aacatattta aatctgtttt gaggcaacca ctaatttatt ttattagatt
     3121 ttgttgatat tttagttttc aaaaagtatt tgattaaacc agatataaaa tgtcaaaaat
     3181 aaaaattaac tgagttaaat aattttaata aaattggtag tatatgtgat aaaattaaat
     3241 attaaagagg atataggata ttagaatatc aattggtgaa ataatgtcat atacgataaa
     3301 ttaaggtgta agtttttggg tatacctatc ttaggagtga aaagatttga gaataagtta
     3361 tagaaattta aagcgatata gagaaattgg atatgtttta ataaagtatg gttttagttt
     3421 tattgtagaa agactaaata tagaaggaat tgcctataaa ataccactat ttgacccacc
     3481 agaagaaata aaaaatatga caactggtga gagaatgaaa agagttttag aagaactagg
     3541 acctacatat ataaaaatag gacagatatt gagtacaaga aaagacttgt tagaccagga
     3601 tataattgat gaaatatcaa aactcagaga tgatgtagag aaatttgatt caaatatagc
     3661 tattgatata tttaaagaag aagtgggttt aagtatagaa gaaatattct tagaatttaa
     3721 agaagaagca atagcagcag cttcaattgg tcaagtttat gaaggtgtac ttaaaacggg
     3781 agaagaagta atcgtaaaaa ttcaaagacc aaatatagag aaaataataa aatctgattt
     3841 agagattctt agaactattg caaatacttt aaaagattta aaaaaggatt ttaatttaga
     3901 tttagtacag atgattgaag aatttcaaac tcaattaatg agggaacttg attatacatt
     3961 tgaagctata aatgcaacta aattctctag gatatttaaa aatagtgatg aagtatatat
     4021 acctaaagta tattcagaat ataatactaa gaaaatttta gtaatggaga aagttaatgg
     4081 tacaaagtta agtgatgtag aaaaaataag gagactaggt tataacacaa aaactattgt
     4141 tgaaataggc gttaggtcat tttttacaca ggtattatct catggatttt ttcatgcaga
     4201 cccacatcca ggtaatatat tcgtagttgc taaaaataag atagcatata tagattttgg
     4261 aatgattgga attattgata ataaaacttt aaaccaatta aatgaaattg cattagcagg
     4321 tgtagaaaag aatgttgaca agataatata tctattgata gaaatggatg cattaaatgg
     4381 agaggctgat attaagggac ttagacagga tttactttat ctaatacatt attactatga
     4441 tatttctata gaaaaaataa atgtaacaga tatattaaat gaactattta ggttttttag
     4501 acagtataaa atagtcatgc ctgcacaatt tgtaactctt gcaaagacag ttataacatt
     4561 agaaggaaca agtagaactt taaacacaga tttttctttt ggttctatgg gaaaagagtt
     4621 tatgaaacat cattataaga gtaaatttaa ccctaaaaat gttgttttaa gttcaagaca
     4681 aaatgtagaa gaaatattac tggacataaa aactattcca aagcaattaa aagcaatttt
     4741 aaaaaatata gaaagaaata atataaaaat gcaaattgaa gatgttaaaa tgacgaggtt
     4801 agaaaattgt ataatagagc taacatctca aatatctttg agtttagtgt tggcatctat
     4861 tatagtaggt tcttctctta taattgcatc tccaaatata gaaaataata tatggataaa
     4921 atttactgca atagcgggtt tctttatatc ctttataata ggcctttgct tagttataag
     4981 aagtattagg tcaaaataca aaaaagatta gggggaatta aatttgaaaa aatggatatt
     5041 gaagtataaa ggtaagatag aggaaagtga atttagcaaa aaacttaata tttccccgga
     5101 aatctgccaa attttaaaaa atagagggat atgtactgaa aaagattcag agatatttat
     5161 gaatccatct ttagattatt taagagaccc ttttttaatg aaagatatgc aaaaagctgt
     5221 tgatagaata aagttagcag ttgagaagaa cgaaaaaatt tggatttacg gagattatga
     5281 tgttgatggt gtatcatcta catctatctt gtgcttatat tttaagagta taggttacaa
     5341 tgttgattac tatattccta atagattaga agaagggtat ggtataaatg aagatgctat
     5401 taagcttata agctctaggg gatgtgactt gattataagt gttgactgtg gaattacatc
     5461 agtgtctgag gtcaatacag cgaatgattt gggcatagat gttataatta cagaccatca
     5521 tgaatgtcaa agtgaaattc catcagcctt tgcagtaata aatccaaaac aagaagattg
     5581 taattatcca tttgattcac tatgtggatg tggtgtagca tttaaaatga ttcaagcctt
     5641 gactccaaaa gaagaattta aaactagtat gtataattat ttggaaatag taacactagc
     5701 aactatttgt gatattgttc cacttataga tgaaaataga ataattgtaa aaaatggttt
     5761 aaaatcaatg aaagaaggca aaaatattgg attaagggag cttataaaag tttgtggtgt
     5821 agaaagtgat aagataggtt cttctcatat aggttttgca ataggtccta ggataaatgc
     5881 ttcaggtcga ttgggatatt cttacttagg agtagagcta tttactactc aatcacaaga
     5941 agaggcagta gaaatagcta gtatattgga agaaaaaaat aatgaaagac agatgataga
     6001 agctaagatg tatcatgaag ctgaggaaat gctaaagtct aatagcagat acaatgatga
     6061 caaggtgttg gttttagcta aagagggttg gcaacatgga ataataggaa ttgtagcatc
     6121 aaagttaact gaaaaatact acaaaccaac aattcttcta ggtatagaaa atggggaggc
     6181 tactggttca gcgaggtcaa taaaaggatt taatatattt gaagctctca taaagtgcaa
     6241 agacttgatg actaagtttg gtggtcatga acaagcagca gggttatcct tggattcaga
     6301 caatgtagaa attttagcaa atgaaataaa taaatttgca gattataatt taactgaaga
     6361 tgatatgata gagaatgtaa atgttgagtt tgaattacaa gaaaatgtaa taaatttaaa
     6421 tcttgtagaa gaattacata agttagaacc ctttggatta aataatccta atcctagatt
     6481 tatagttaga aattatattt taaaagattt gaaagttata ggtaagaatc aacaacatct
     6541 gaaattaagc atagaaaaag agaaaagcta tgaatgtata ggatttaata tgagtcattt
     6601 aaaatcaatg tataaagtag gagacaaggt agatgtacta ttccaattag atgaaaataa
     6661 ctatatggga aataggaaag tacaattttt acttaaagat atacgattgg ctcgacctaa
     6721 aagtgcaagt aatgacaaac tttcattgaa attaatgtca aaaataatac caaaagatac
     6781 tcaaagcctg tataatatat ctgtgtctga ttttgaatta tttgatggaa atacagatat
     6841 aaatatattt gattattttg aaaaagatac actaataata tctaattcta taaatggatt
     6901 ttatagagca atgtcagata tatcacttat agatttggat tttaacataa attacaatat
     6961 tattgaagat gttagcaaaa atactgataa attagagctt atattttctc ctaatattga
     7021 taaaatagac ttaaaaagat ataataatat tattctttat gattatttat acaataaagg
     7081 cgaatattcg tatatatatg agaataaaag agaagaaagt gaaattataa aatattataa
     7141 taaaacagac ctattatatt taaagaatgt agttagtaat atagttccat caagagatga
     7201 atttataaca atttataaac aagcactcat aaaaaaagaa attgatttgg atatggtaaa
     7261 tataagggaa acttttaatg taattccatt aaagtttttt acgatattaa atgtatttag
     7321 agaattaaat ttacttgact ttaatttaaa ttatgaaaaa aattctgtac tcataagaat
     7381 attgccaaaa ccacagaaaa aattagactt aaatgagagt ttaatactca ataatctaaa
     7441 aaatttagaa aaacaataca atagcagtta ttaggaggcg tttcccatgg atttaaaaaa
     7501 ctttataagg aatattgatg atttcccaaa gccaggcata gattttaaag atgtgacaac
     7561 tctatttaaa gatggagatg catttaaata tgcagtggat tctatagttg aagaattgaa
     7621 agataaagat gtagatttag taataggacc agaagcaaga ggatttttga tgggtacacc
     7681 tgtagcatat gctttaggag taggatttgt tccaattaga aaacctggaa agttaccagg
     7741 tgaagttgag agttatgagt atggattaga gtatggtaca gatactttag agatacataa
     7801 ggatgcaatt aaaaagggtc aaaaagtagc aatagtagat gatttattgg ctactggtgg
     7861 aactatggaa gccgctgcca agcttgtaga aaaattaggt ggagaagtag tttctatgca
     7921 atttttgatt gagctaaaat tcttaaatgg gagagaaaag ttgtctaact atgatgttaa
     7981 ttctcttata aaatactagt acttttataa tagttggtat aacaccaact attttcatat
     8041 atattaaaat tttatacaaa ataggtggtt atatgcatga taaagaatta caagaaataa
     8101 ttgaaaaaat aaaagtttat gcacctaatg caaattttga aatggttgaa aaagcctata
     8161 atcttgcaaa aaaagcgcat gaaggacaat atagaaaatc tggggaacca tactttattc
     8221 atccattagc agttgcaaat attttaatag agatggaatt agatattgaa actataacag
     8281 caggtttact tcatgatgtt gtagaagata cagaatatac ctatgaagac ataaaaaaag
     8341 aatttggcaa agaagtagcg gatttggttg atggagtaac taaacttggt caaataaaat
     8401 atcgttcgaa agaagaaact caatctgaaa atttaagaaa aatgtttttg gcaatggcga
     8461 aggatataag ggttatactt ataaaacttg ctgatagatt gcacaacatg agaacattaa
     8521 aatacatgcc tccagaaaaa gcaaagtata aagcaaaaga gacacttgaa atttatggtg
     8581 gtatagctca tagattagga atttccaaga taaagtggga actagaagac agagcattaa
     8641 gatttatgga cccagaaggg tattatgatt tagttagtag agtatcaatg aagagaagtc
     8701 aaagagagga ttatattcaa ggaattgttg aacttcttaa agataagttt aaagatttaa
     8761 gtataccatg tgatgtatat ggtagaccaa agcattttta cagtatatat agaaagatgc
     8821 aaaaaaaaca taagactttt gaagagattt ttgatttaac agcagtaagg atattggttg
     8881 ataatgtaaa ggattgctat gcagtacttg gtatagttca tacattatgg agacctattc
     8941 caggaagatt taaagattat atagcaatgc caaaacccaa tatgtatcaa tcattacaca
     9001 ccactgttgt aggtcctgat ggagaacctt tagagataca aataagaact catgagatgc
     9061 ataatatagc tgaaaatggt atagcagccc attggaaata taaagaaggt atctctagca
     9121 aagaatcaaa ggttgaagaa aaacttcaat ggttgagaca gatgatggag tgggaaaaag
     9181 atttaaaaga cccacaagag tttatggatg cacttaaaga agatgtattt aatagtcaag
     9241 tttatgtatt tacgccaaaa ggagacgtaa ttgaattacc agcagaatca acacctatag
     9301 attttgccta tagggttcat acaaatgtag gcaataaatg tgtaggagct aaaattgatg
     9361 gaagaatagt tcctattgat tataagcttc aaaatggtaa tattgtcgag gtacttacat
     9421 catctaattc taatggacca agtagggatt ggcttaatat agtgaagacc ccaaatgcaa
     9481 aaagtagaat tagacaattt ttcaagaagg aaagaagaga agaaaatgtt gaccgtggaa
     9541 atacaatatt agaaagagaa tttaagaagc atgatatacc acaaaaagac cctcttgttg
     9601 aaaaatatat ggcattagtt gctaagaaat ttaatcagcc aagtgttgag gacttaattg
     9661 ccactgtagg ttatggtggt ataatgtcat ctcaagtggt atctaaggta aaagacctat
     9721 atttaaaaga ggttaaaaaa gctaaaaagg aacaacagat acaagaagaa gatttaaata
     9781 aacacaatat aagtgaaaca gaatacagta aaaagagaaa gaaaaacgca tcacaaggaa
     9841 ttatagtaaa agggcttgac aatatcttag taagatttgc taagtgttgt aatcctcttc
     9901 caggagatga aattgtagga tatattacta agggtagggg agtagccata cacagagctg
     9961 attgcccaaa tgcaaacaat aatggtgatt tcttccaaaa tagattggtg gaggtttcat
    10021 ggagttcagc aggaaaaggt aagtttgaag cggaaattca aataaaagct attgatagaa
    10081 gaggaattgt taatgaaata actcatattg ttgcaatgga taaacttagt ttaaacggaa
    10141 taaatgcaag aaaaggtaag gatagtattg taagtataaa cttacttgta gaggttaatg
    10201 acacagagga attaaaaatt ctaatgaaaa aattaaaagc aattcctggt gtagaaagta
    10261 tttatagaat gacaaattag agttaaaata atagggagga aactatgaga gcagtagtgc
    10321 aaagagtatc ttcatctaaa gttacagtag atgaaaacac tataggtcaa ataaataagg
    10381 ggttactagt attgttagga gtgactcatg atgataaatc aagtgatgta gattatatga
    10441 ttgataaaat attgaatctg agaatttttg aagatgaaaa tgacaagatg aatttatctc
    10501 taatggatat aggtggagag cttttagtag tatctcaatt tactttatat ggagattgca
    10561 gaaaaggaag aagacctgga tttagtaatg cagctaggcc agaacttgca aataaccttt
    10621 atgaagaatt tgtaaaaaaa gcaaaagata aaggtgtgac agttggtaca ggtcaatttg
    10681 cagctcatat gatggtagag ttaactaatg atggtccagt aacaatacta ttagactcaa
    10741 gtaaatcttt ttaaggggga aaactttaat gattataaaa aaatttgtag atagttattt
    10801 tggagtaaat acttatgtat taggtgatga aaagactaaa aaatgtgcag ttatagaccc
    10861 aggtggaagt ttagtagaga cattaagttt tgtaaaagaa aatgaattaa atattgaata
    10921 tattatcctt actcatgggc atggagacca tataggatat gttaaggata ttaaagaaaa
    10981 aactggtgct aaagtagttg cacatgttga tgaaaaagaa ttattaaatg acaaaaataa
    11041 aaatctaagt tatacaatga gatgtggagc gcaagagttt gatgcagata tctatgtaaa
    11101 tgataaagac aagcttgaac ttggtgaatt aaaaatgact ttcttgcata ctccaggaca
    11161 tactcaagga tgtatgtgta taagagttga agatgaaatg ttcacaggag atactttgtt
    11221 tgctggaagt attggtagaa ctgatttata tagtggagac tttaaccaaa tggaaaaatc
    11281 acttaagaaa ttatgtaaat atgaagatgc tgttagagta tatcctggac atggaccaac
    11341 cagtactcta ggggttgaaa agaagacaaa tccttacatg taattttata ttagtattta
    11401 aaaaagtata aaatatttaa taactatatt agtgaggggg ttcttctact gttaaataga
    11461 aggagttaag ttgatgttgt atgttttttt aaaaggacat gattataagt atgaagtagc
    11521 agaacttata aaattgttta ctagtgaatt taagtttaaa gactctaatg ctaacaataa
    11581 tatagagtca aagcatttaa taaatagatt gatttatgag aatggtgtat tattctcaag
    11641 tactgaatac tatgaaaatg gaaacttaaa gtacgagtca atacagaata tagaaaatat
    11701 gaatcttgaa tttttagatg gatttgatag cagcaaaacg attgaagaat ttcttaatag
    11761 cctagatagt gaaagtaaga ggaactttaa aaagctgtgt aaagaaaata tcaaaaaaag
    11821 catgttttta gtgcttaaac aagtatttaa ttcttatgta ccatggggaa ttttgactgg
    11881 tattagacca gtcaaaattg tccacaattt aatggataaa aagctagatg ataatagcat
    11941 aagaaccatt ctaaaagata attattttat aatagatgag aaaatagatt tagctcttga
    12001 aatagctaaa agagagcgat tatttatgta tcctatagat aaaaataaaa tttctcttta
    12061 tgtaagtatt ccattttgtc caactagatg tgtatattgt tcatttcctt caaattcatt
    12121 aaaacaattt ggacatttaa aaagagaata tgtttataaa ttgattgaag aaattaaggg
    12181 gtttgccaaa gttattaagg atactaagaa agaagttgaa actctatata ttggtggtgg
    12241 aactccaact acacttgatg aggaagaatt ggatttgctt ataaattcat tgttcaatga
    12301 attagattta agtaaaatta aagaatttac tgtagaagca ggtagacctg acactattac
    12361 agaacaaaaa ctaagagtgt taaaaaaaca caatgttagt agaattagta taaatcctca
    12421 aactatgaat gatgatacct tggtaaagat tggaagagag catagagtat ctgatttagt
    12481 agattgcttt aatatggctc gtagaatggg atttgataat ataaatatgg atataatttt
    12541 aggtcttata gatgaagact taaatatggt aaagaacaca ttggaggaga taaaaaaact
    12601 ttctcctgaa agccttacag ttcatacatt agccataaag agagcatcta acttaaatat
    12661 aaatatggat aggtataaag aacatctaac acaatatgaa gaaatggtaa aaatgataga
    12721 tttatcaatg aaatatgcaa aagatatggg attaaatcca tattatatgt acagacaaaa
    12781 acatatgttg ggaaatttag aaaatatagg ttatgctaaa gaaggatatg agtgcattta
    12841 taatatacag attatggaag aaaaacaaag taactatgca ttaggtgcag gtgctatatc
    12901 gaaatttgtc tatgtagatg aagatagaat tgaaagagta gaaaatgtaa aaaatgttga
    12961 acaatacatt gaaagagtag atgaaatgat aaaaaggaag aaagaagagg tatataaaaa
    13021 tgttgactaa agctcctaga ggaactaaag acataactcc aaaagaggct tacaaatggc
    13081 gttatgtaga aaataaattt agagaaatat gtgctttata tggatatgaa gaaatggtaa
    13141 cacctatatt tgaacataca gaacttttca agagaagtgt tggagatact acagatatag
    13201 ttcaaaaaga aatgtattct tttaaagata aaggtgatag agaaattacc ctaaagccag
    13261 aaggtacagc aggtgtagta agagcattta tagaaaataa attatatgca gatactcaac
    13321 caacaaaatt attttatgtg acaccttgtt ttagatatga aagaccacaa gctggtagac
    13381 aaagacaatt tcaccaattt ggaatagaag cacttggaag tgatactcct tcaatggatg
    13441 ctgaaataat cgcactagct gttcaatttt ttaatgaagt tggattaaat gatttggtag
    13501 tgagtataaa ttcagttggc tgtccagttt gtagaaaaga gtacaatgct ttattaaaag
    13561 agtatcttga ctcaaaagct gatatattgt gtgatacttg caatgagaga agagaaaaaa
    13621 atccaatgag ggttatagac tgtaaaaatc ctacatgtaa agaaaatatc aaagatatac
    13681 catttattgc agaccatttg tgtgatgatt gtaaatctca ctttgataaa cttcaagaat
    13741 acttaaagga aatgaatata aactttgtaa ttgataaaac aatagttaga ggcttagatt
    13801 actatagaaa aactgctttt gaaattatat ctaatgatat aggagctcaa agtactgtat
    13861 gtggtggagg aagatatgat ggtcttgttg aacaattagg aggaccaaaa ggtataagtg
    13921 gaattgggtt tggactagga attgaaagac ttttacttac acttgaaggt aatggaattg
    13981 aaatagaaaa ccctcaatct acggatatat ttatagtaac tataggtgaa gaagcaaata
    14041 caagaagctt taaattgtta aaagacttga gacaaaatca tataagtgca gataaagacc
    14101 atatagagag aagtgttaaa gcacaattta aatattcaga taaaataaat tcaaaattta
    14161 caatagtaat tggagatgat gaacttaaaa atgacactgc gacattaaaa aatatgaaaa
    14221 catcagagca aactactgta aaattaagta cattagttga agaattaaag caaaagctgt
    14281 aaaataagaa agtatgatgt tttgtgtatt agataaatat atagcattaa aattaaataa
    14341 aatggaggta gtatcttgga aactttaaag ggtttaaaga gaactcacta ctgtggagag
    14401 ttgagagaaa aaaacataaa tgaagaagtc gtacttatgg gatgggtaca gaaaaaaaga
    14461 aatttaggtg gtcttgtatt tgttgattta agagatacaa gtggattatg tcaaatagtt
    14521 tttgatacag atgtagataa agaagctttt gaaaaagctg aaaaattagg ggctgaattt
    14581 gtaatagctg taaaaggaaa agtatgcgaa agacaatcta aaaaccctaa tatgccaact
    14641 ggtgatatag aaatatttgc aactgaactt agacttttaa acaagtcaga aactccgcct
    14701 atttatataa aagatgatga tgatgtttct gaagcactaa gattaaaata tagatactta
    14761 gatttaagaa aaccatctat gcaaagaaat ttaaaattaa gacataaagt aatgaatatt
    14821 acaagaaatt acttatcaaa caatagattt tgcgaaatag aaacaccatt tttaatagct
    14881 ccaacaccag aaggagcaag agattatcta gtaccaagta gagttaatcc aggaaaattt
    14941 tacgcattac cacaatcacc acaattatat aaacaattat tgatggtaag tggtatggat
    15001 agatatttcc aaatagttaa atgctttaga gatgaagact taagagcaga tagacaacca
    15061 gagtttacac aaatagactg cgaaatgtct tttgttgagc aagaagatgt tatgagtatg
    15121 attgaaggtt tgcttgaagc gatatttaaa gaagttttag atgtagaatt ggcacttcca
    15181 cttcctaaga tgacttatgc agaagctatg tctaaatatg gttcagataa accagatact
    15241 agatttgggt atgagttaac agatatatct gatgttgttt gtaactgtgg atttaaagtg
    15301 tttgctgatg ctacacaacc aggaaaaagt gttagaggta taaacgtaaa aggcaaagct
    15361 gacgacttta ctagaaaaca aatatcatca ctagaagaac atgctaagac atatagagca
    15421 aaaggtcttg cttggatgaa agtaggacaa gaaggagtaa cttctccaat agcaaaattc
    15481 ttcaatgaag aagaaatgaa cgctatactt acaagaatga atgctgaagt aggagattta
    15541 cttttatttg tagctgataa aaactcaata gtatttgatg ctttaggaca agttagactt
    15601 gaagtagcaa ataggcttaa tctattggat aaaaatgtat ataacttact atgggtaact
    15661 gagttcccag tttttgaaga agatgaagaa acaggcacat tctcagcaat gcatcaccca
    15721 tttacatcac ctatggatga agatttagac aagttagaag aaggtgataa gtcatcttta
    15781 agagcgaaag cttatgatat tgtattgaat ggttatgaaa taggtggagg aagtgttaga
    15841 atatcaaatt ctgatgttca atcaagaatg tttaaagcac ttggttttac agaggaaaga
    15901 gctaatgaaa agtttggtta tttattagaa gcatttaaat atggtactcc tcctcatgct
    15961 ggtctagctt ttggtcttga tagacttgtt atgttacttg caggaactga taatataaga
    16021 gaagttattg cattccctaa aaatcaaaat gctgtttgcc ctatgacaaa tgctcctaca
    16081 ttggcggaag atgagcaatt agaagaatta agtataaaag ttgatataaa agataatgaa
    16141 taatatgtag ttgcaagaaa ctaaaatgta aaaagtctgg atataaaata tatttatatt
    16201 cagacttttt ttattagaat tattttatat tttttctcta tatattttca taaattatac
    16261 ttatattctt aaccaatata attaaaatac tatgtttaaa aattttacat aaacttggtt
    16321 gttttagaaa ctacttctgt aacgagtaca c
//
LOCUS       DAYAPA010000094        16271 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.094, whole genome shotgun sequence.
ACCESSION   DAYAPA010000094 DAYAPA010000000
VERSION     DAYAPA010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16271
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.094"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            245..1693
                     /locus_tag="ACGI59_002754"
     CDS             245..1693
                     /locus_tag="ACGI59_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HFG9740534.1"
                     /translation="MKLGKDFLWGGAISANQSEGFYLDGGKGLSQFDKLPLNDKRLKN
                     VCLDEDNILNENNQYYPSHIGIKFCENYKEDIAMLAEIGFKCFRFSISWSRIFPTGEE
                     SEPNEKGLQLYDNIIKELKKYNIEPLITISHFDIPYSLVEKYSGWADRRVVNLYVKYA
                     EILMKRYKDDVKYWIPFNEMNMIMHIPYIGAGLTFIADENQLQKKYQAAHHQLLANAK
                     TIEIGRYINKEFKFGCMLAAGKTYPYTCKPEDVFATLEHERHNLFFSDVQVRGVYPSF
                     MSYYLKSNQIKLIIKEEDLSTLKDNTVDFVSFSYYSSACSSARAEGLEKSKANGPETI
                     KNPYLPKSNSVWQNDPVGLRITMNQLYERYQLPLFIVENGLGTQDIVNEDGKIIDDYR
                     IDYMKEHIKNMILGVNEDGIELIGYLMWGCIDVTSVSEGKMTKRYGIIHVDSDDYGRG
                     SFKRTKKQSAFWYKKVIETDGEILFNDMETHA"
     gene            1719..4109
                     /locus_tag="ACGI59_002755"
     CDS             1719..4109
                     /locus_tag="ACGI59_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HFG9740535.1"
                     /translation="MSLIFTTKDQLVELGFSKDTIKSNETLFTLANGHLGVRGNIEES
                     DFNNEYVDNMGTYVNGFYEESPITYGESAYGYAKQNQTICKLPNAHIVNFSIEGDCFD
                     LSKGITSEHTRILDLNEGILKRSFIWENSRGRKVKVSIERLVSQDIEELMMISYTITP
                     LNFNGKIKFKNKMENSMEKFNCEKVDDPRVASINKKQFKINVKKYNNKYFMNLVTERS
                     RLGLWCGFDSYITCGNITNETYNVSDECIEREIEVYAKKDERLNLEIVVGYSRVYSDL
                     NLNEERLKSLECLESLKCLESEAHLKSLELVLREACNIGFDKVKNIQRKKMNEFWTTS
                     DVEIEGDKTLQLGIKTNLFHIYQSAGRDGKTNISAKGLTGDGYEGHYFWDTEMYILPF
                     FIYTQPSIARALLDYRYSILDASRERARELGINKGCLYSWRTINGNECSAYYPAGTAQ
                     FHINADIAYGVRTYFEATNDEEFMMQNGLEILIETSRFWVEFGDYIESKNNKFCINGV
                     TGPDEYTAIVNNNYYTNLMAKYNLIYAVQMVDKFKHNLFSNEVFRKVKLQEYEVKNWI
                     NAAQNMYLPYDEEKKISKQDDSFLEKSVWDFKNTPKEKYPLLLHYHPLTIYRYQVNKQ
                     ADTVLSALLFPDEFSLEQKKRDFEYYEKITTHDSSLSRSIFGMMASEIGNHEKAYNYF
                     MDTALMDINNLQKNTSDGIHAANMGGTWMSLVYGFAGMKIKNDKLSFEPRIPKHWKNI
                     KFNILFRDNLVFVNIEEYKSYYKLIKGRNITILHDNKEIKLSKNIIEVNNCFKGSN"
     gene            4111..4782
                     /gene="pgmB"
                     /locus_tag="ACGI59_002756"
     CDS             4111..4782
                     /gene="pgmB"
                     /locus_tag="ACGI59_002756"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFG9740536.1"
                     /translation="MKAVLFDLDGVITDTAKYHFEAWHEIAKSLDIEIDETFNEKLKG
                     INREESLDKILLSANKVDSYTKEEKLKVCEEKNNIYIKLINSLTYKDILPGINELIKE
                     LKKNNVKLGIASASKNAPRILESLGVKDEFDCIVNPSLLKRGKPYPDIFIEGSKMLNL
                     KQYDCVGIEDSYSGIKAINAANMTSIGVGKKEVLGKCDHIVEDTSLLNYNTLLQAWSK
                     KINLD"
     gene            4821..6191
                     /locus_tag="ACGI59_002757"
     CDS             4821..6191
                     /locus_tag="ACGI59_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFG9740537.1"
                     /translation="MKNKYEDVAQLIIDGLGGKDNIIDLTHCMTRLRFILKDEKKVNA
                     DKLKSIDKVVGVNSTSTQYQVIIGNEVGVVFNAIINKGINTTGDIKSDIKNKNKKNSI
                     LNNIIDTITGCMTPMIPALTASGMIKVILSLCTTFNWLSSNSSTYRVLDIIGDVAFYF
                     MPIILAINAAKKFNVNTSIAVIVVGVFLHPNFSAWVSSGDPISFIGVPIQGVIYAASV
                     IPALLTVWMMSYIENFIDKLTPSMLKTILNPTLVLLISAPIALIVIGPIGNFLGEGLA
                     SIINLLQGRLGFIMVCLLAAAMPFIVMTGMHHALTPIFISAFATTGQESLILISQVCA
                     NLAQGGATLAVALRSKDSKMKQLASAAWISAIMGITEPALYGVTLKLKKPAMLASIGA
                     GIAGLFAGITHVTLYVPQNSIMAVLGLSGEKGISNIVNGLIVIFIALVAPFIMTYVFG
                     VEKNVK"
     gene            6538..7380
                     /gene="licT"
                     /locus_tag="ACGI59_002758"
     CDS             6538..7380
                     /gene="licT"
                     /locus_tag="ACGI59_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HFG9740538.1"
                     /translation="MIINKILNNNVVITLDDNDEEVIVMGKGIGYQKSKGNLIDKTKV
                     NKVFRISNKEISNKLQELLNNIPIEHMKLSSEIIEYAQIKLNKKLNESIYISLSDHTY
                     SAIQRMKEGINVKNAILWEIKRFYKEEFEIGMKALDIIENKTGIKLPEDEAGFIAFHI
                     VNAQLSEGHTLASDITKLIQEVLSIVRYHFRIEFHEESVFYYRFIMHLKFFAQRLLLD
                     SAHEGETDKELLSIIKSKYNKEFECVVKIKNFIKKQYNYILTDDELIYLTIHLAKVVK
                     DSNM"
     gene            7525..9453
                     /locus_tag="ACGI59_002759"
     CDS             7525..9453
                     /locus_tag="ACGI59_002759"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFG9740539.1"
                     /translation="MGKYKQLAQEIVNNVGGKENISGLVHCITRLRFTLKDESIANDN
                     VLKNMEGIVTVMKSGGQYQVVIGNHVEAVYKDVVEIIGLDDSSTSSETKKSGNILDKG
                     IDIISGIFQPVLGIMAACGMLKGFNALFVAMGLYSDVSGGYMVINAAGDALFTFLPLF
                     LGYTSAKKFGLKPMLGLALGAAVCYPAIQGSSISAGADVMYTLFKGTMFASPVYIDFF
                     GLPIVSIDYTGTVVPIIFIVYFASRCEKLFNKCVPNLVKFFFVPMLTLLITVPVALIV
                     IGPIATFGSTLISQIVISIRDFSPLLAGAIVGFTWQILVIFGMHWGFIPVYINNVMTL
                     GYDNVMMPFFACTFASSAVVLAIFFKTKDKKLKEMAIPNFISGIFGVTEPAIYGILLP
                     LKKPFIISCIASGIGGAFYGFFNLRKFITGGMGIFELPAMIEPDGGMGNLIVALSGIA
                     ISMVVAFVLTMILYKDEKVEESKKVRNKGKEEIKEVKSTKLEREIVTSPIKGEVLKLS
                     DIEDAAFASGVLGQGVAIIPSDGKVVAPVDGVVTTLFPSLHAIGILSDTGVEVLIHIG
                     LNTIQLEGRGFEACIKQGDRITKGQTILNFDVDAIKELGYSTVTPIVITNSSQFLDVV
                     ETESKNIELEDNLITVLF"
     gene            9514..10941
                     /locus_tag="ACGI59_002760"
     CDS             9514..10941
                     /locus_tag="ACGI59_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFG9740540.1"
                     /translation="MSFKNDFLWGGATAANQCEGGYNEDERGLANVDVCPTGKDRTAV
                     ITGKLKMFDFDDEHYYPAKTGIDMYHNYKEDIKLFAEMGFKVYRMSIAWSRIFPKGDE
                     ETPNEKGLQFYENIFKECKKYKIEPLVTITHFDCPMHLVKKYGAWRNRKMIGFYEKLC
                     NVIFRRYKGLVKYWLTFNEINMILHAPFMGAGICFEEGEDVEKIKYQAAHHELVASAI
                     ATKIAHEVDSNNLIGCMFAAGSVYPYSCNPNDVWEATKLDRENYFFVDVQSKGKYPNY
                     ALKYMEQKGITPEMEPGDIELLNKYTVDFISFSYYNSRCVRTDENADDMAEGNIFTSA
                     KNPYLSYSQWGWPIDPLGLRITLNHVYDRYEKPLFVVENGLGAKDIADENGYVEDDYR
                     IDYIREHIKAMHDAVSIDGVDLLGYTTWAPIDLVSAGTGEMEKRYGFIYVDRDNSGNG
                     SLRRMKKKSFEWYKKVIASNGEDLY"
     gene            11352..12128
                     /locus_tag="ACGI59_002761"
     CDS             11352..12128
                     /locus_tag="ACGI59_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFG9740541.1"
                     /translation="MKYQHIIEVDKELCIGCGLCKNDCPVNNIIIENKKSVIKKQDCL
                     MCGHCAAICPTKAITLTGFDEPPIELTNKPKLDSDELLMAIKSRRSIRKFKDKEVSSE
                     IIKQIIEAGRYTPSAKNSQDVSYIVLDNKKSIYENEAVKFFRKIKPIANIAIKYSKEV
                     EIDDNFFFKHAPIAIMIITKDKISGSLAASNMALMAESYGLGVLFSGYFSDVANNSPK
                     LKKLLGLKRSNHVLTTLVIGYPDVKYRRTAQKEVATVRYL"
     gene            12144..12509
                     /locus_tag="ACGI59_002762"
     CDS             12144..12509
                     /locus_tag="ACGI59_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFG9740542.1"
                     /translation="MEKECQQRMQEITSKFKECRKAISAMGDETRQLILIALLESDFN
                     GIRVGEITKKTHLSRPAVSHHLKILKEAEIVNLRREGTKNYYYLDSKKSQWRSLTELI
                     NLIFVGIQHISENDIRKGD"
     gene            complement(12685..13434)
                     /locus_tag="ACGI59_002763"
     CDS             complement(12685..13434)
                     /locus_tag="ACGI59_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440038.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HFG9740543.1"
                     /translation="MILYFSGTGNSRYVARKIAQELNDELISLNQLIKEEKTDELIST
                     DKPFIFVCPTYAWRLPIVVTDFIKETKFLGSNRVYFVMTCGGDTAKSINYIQKLCKYK
                     EWQLKGMAEIKMPENYIALFSTTDKETSKQMIKEADKQIYRIISDIRNENEFEIITPS
                     GLGGTIKSGIINTVFYKTIISAKGFHYTDKCIGCGKCVELCPLNNINLKNKKPVWKNN
                     CTHCMACICGCPTEAIEYKNKTQNRERYYLE"
     gene            13656..13784
                     /locus_tag="ACGI59_002764"
     CDS             13656..13784
                     /locus_tag="ACGI59_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9740544.1"
                     /translation="MKDYKYKGYFEGVEIFDNVNDLNLNTLTTVSRFVFVFLFTIT"
     gene            13912..14094
                     /locus_tag="ACGI59_002765"
                     /pseudo
     CDS             13912..14094
                     /locus_tag="ACGI59_002765"
                     /inference="COORDINATES: protein motif:HMM:NF024835.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            14122..14337
                     /locus_tag="ACGI59_002766"
     CDS             14122..14337
                     /locus_tag="ACGI59_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006774905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HFG9740545.1"
                     /translation="MVPPKGKFFFGAVVVGERGQIVIPKEAREVFNINAGDKLLVLGD
                     EERGLGIVHQRDLINFIGHVGVVHKEE"
     gene            14369..15556
                     /locus_tag="ACGI59_002767"
     CDS             14369..15556
                     /locus_tag="ACGI59_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide family glycosyltransferase"
                     /protein_id="HFG9740546.1"
                     /translation="MSKIVFFSIPAYGHTNPTIEVVRELVDRGNEVLYYSFNEFKDKI
                     EGAGAKFICCDKYLPELLPGDEKKIGKDFPGLIEMIVDTTISLDEKVCRELKDFNPDC
                     IVSDSLSFWGKLFAQKLNIPYVCSTTTFAFNKHTAKLMKQNFIEIIRMIFGVRRINKK
                     IKLLQRKGYEVKNFISIVSNDNDTDTIVYTSKEFQPMVETFSSKYSFVGPSVSKLIIE
                     PKERKRKLIYISLGTINNKNVSFYKNCINAFKDSDVDVIMSVGRSTDIKSLGNIPNNF
                     EVKNSVEQIAILQKTDVFVTHSGMNSVNESLYYGVPMVLFPQHSEQRMVAKRVVHLGA
                     GIMLKEDKSESIKKAVFQVINDNEYKENATKLSKSFYNAGGSKKAADVILQIICNSRQ
                     VDI"
     gene            15748..16152
                     /locus_tag="ACGI59_002768"
     CDS             15748..16152
                     /locus_tag="ACGI59_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3139 domain-containing protein"
                     /protein_id="HFG9740547.1"
                     /translation="MKKYKVIFIVLIIGIISVTGFVVYKNCIKPWKDAETYIDKYMVK
                     QDISKDNIKSITKEKAKKASYEGILYKVYYKDDPRYEYQYFYSDNYYALYVNKVMLQI
                     YDMENNNKLLRSNEELKSVKYPPIYLKYKPDK"
BASE COUNT         6410 a         1703 c         2870 g         5288 t
ORIGIN      
        1 gagaataaga taacaataat gaggatataa aaatgtattt gctatatata taattatata
       61 catagatatc taatattgat atttaatatg aaagtattga aaatagacaa tattaattat
      121 agtgagtaaa aaatataatt ggatttttaa attgaattat ttaaaaggta tagtatgaaa
      181 aaaatcttat aactaaatat gagatagaaa ttataaaatt ttatactgtg aaagaggtag
      241 atgcatgaaa ttagggaaag attttttatg gggaggggca atttcagcca atcaaagtga
      301 aggattttat ttagatggag gaaaaggttt atctcagttt gataaactcc cattaaatga
      361 taagagatta aaaaatgtat gtttagatga ggataatatt ttaaatgaaa ataatcaata
      421 ctatccaagt catataggga ttaaattttg tgaaaattac aaagaagata tagcaatgtt
      481 agcagaaata ggttttaaat gttttagatt ttctatatct tggtcaagaa tattcccaac
      541 aggagaagaa agtgaaccaa atgaaaaggg attacaactt tatgataata taataaaaga
      601 attaaaaaaa tataatatag agccattaat aacgataagc cactttgata taccttattc
      661 tctagttgaa aaatattctg ggtgggctga tagaagagtg gttaatttat atgtaaaata
      721 tgctgaaatt ttgatgaaaa gatataaaga tgatgtcaaa tactggattc cttttaacga
      781 aatgaatatg ataatgcata ttccatatat aggtgctggg ttaactttta tagctgatga
      841 aaatcaactt cagaaaaaat atcaagcagc acaccatcaa ttattagcta atgcaaaaac
      901 tatagaaata ggaaggtata taaataaaga atttaagttt gggtgtatgt tagctgctgg
      961 aaaaacatat ccatacacat gtaaaccaga agatgtattt gcaactttag agcatgagag
     1021 gcataatttg tttttttctg atgttcaagt taggggtgtg tatccaagct ttatgagtta
     1081 ttatttaaaa tcaaatcaaa taaaattaat aataaaagag gaagatttat caacacttaa
     1141 ggataatact gtagattttg tatcttttag ctattattct agtgcttgta gttcagctag
     1201 agctgaaggt ttagaaaaat caaaggcaaa cggaccagaa accataaaaa atccatatct
     1261 tccaaaatca aatagtgtat ggcaaaacga ccctgtaggg ttaagaataa ctatgaatca
     1321 gttatatgaa aggtatcagt tacctttatt tatagtagaa aatggactgg gtactcaaga
     1381 tattgtaaat gaagatggaa aaataattga tgattatcgt atagattaca tgaaagaaca
     1441 tattaaaaat atgattcttg gagtaaatga agatggaata gagcttatag gatatcttat
     1501 gtggggatgt atagatgtaa ccagtgtaag tgaaggaaag atgacaaaac gatatggcat
     1561 aattcatgtt gattctgatg actatggaag aggctcattt aaaagaacta aaaaacaaag
     1621 tgctttttgg tataaaaaag taatagaaac agatggagaa attttattta atgatatgga
     1681 aactcatgca taattcattt taagaaagga gtgttattat gagtttaata tttactacta
     1741 aggaccaact tgtagagcta gggtttagta aggatacaat aaaatccaat gaaacgttgt
     1801 ttacactagc taatggacat ttaggagtta gagggaatat tgaagagtct gattttaata
     1861 atgagtatgt agataatatg ggtacatatg taaatggatt ttatgaagaa tctccaatta
     1921 catatggaga aagcgcttat ggctatgcaa aacaaaatca gactatctgt aagttaccta
     1981 atgctcatat agtaaatttc tctattgaag gagattgctt tgatttatca aaaggtatta
     2041 caagtgaaca tactaggata ctagatttaa atgaagggat tcttaaacgt tcatttatat
     2101 gggaaaactc tcgtggtaga aaagttaaag tatctataga aagattagta tcacaagata
     2161 tagaagaatt aatgatgatt tcatatacaa taacaccatt gaattttaat ggaaaaataa
     2221 aatttaaaaa taaaatggaa aatagtatgg agaagtttaa ttgtgaaaaa gtagatgacc
     2281 caagagtggc atctataaat aagaagcaat ttaaaattaa tgtaaaaaaa tataacaata
     2341 agtattttat gaatttagtt acagaaagaa gtagattagg gttatggtgt gggtttgatt
     2401 catatattac ttgtggaaat attacaaatg agacctataa tgtttcagat gaatgtattg
     2461 agcgagaaat agaagtctat gccaaaaaag atgaaaggtt aaatttagaa attgttgtag
     2521 gctatagtag agtatattct gatttaaatc taaatgaaga acgtttaaaa tcattagaat
     2581 gtctggagtc gttaaaatgt ttagaatcag aagcacattt aaaatcatta gaattggttt
     2641 taagagaagc atgtaatatt ggatttgata aagtcaaaaa tattcagaga aagaaaatga
     2701 atgagttttg gacaacctct gatgtagaga ttgaaggcga taaaactctt caactgggga
     2761 taaaaaccaa tttatttcat atttatcaat cagcaggaag agatggaaag actaatatat
     2821 cagcaaaagg tcttacaggt gatggctatg aggggcatta tttctgggat acagaaatgt
     2881 atatattacc attttttata tatactcaac catccattgc tagagcttta ttagattata
     2941 gatactcaat cttagatgca tcaagagaaa gagctagaga acttggaata aataaaggtt
     3001 gcttatattc atggagaact ataaatggta atgaatgcag tgcatattat ccagcaggaa
     3061 cagcacagtt tcatataaat gcagatatag cttatggtgt tagaacgtat tttgaagcaa
     3121 ctaatgatga agaatttatg atgcaaaatg gcttagaaat tcttatagaa acatcaagat
     3181 tttgggttga atttggagat tatatagaat caaaaaataa taaattctgt ataaatggag
     3241 taacaggacc agatgaatat acagcaatag taaacaataa ttactacact aatttaatgg
     3301 ctaagtataa tttaatatat gcagtacaga tggtagataa atttaagcat aatttatttt
     3361 caaatgaagt atttagaaaa gttaaattac aagaatatga agtgaaaaac tggataaatg
     3421 ccgctcaaaa tatgtattta ccatatgatg aagagaagaa aataagcaaa caagacgatt
     3481 catttttaga aaaatcagta tgggatttta aaaatacacc aaaagaaaaa tatccattac
     3541 ttctccatta tcatccatta acaatatata gatatcaagt aaataaacaa gcagatactg
     3601 tactttcagc gttgcttttc cctgatgagt ttagcttaga acaaaagaag agagattttg
     3661 aatactatga aaaaataact actcatgatt catctttgtc aaggtcaata tttggaatga
     3721 tggcaagtga aattggaaat catgaaaaag cttataacta ttttatggac acagcattaa
     3781 tggatattaa taacctacaa aaaaatacaa gtgatggaat acatgctgct aatatgggtg
     3841 gaacatggat gagtttagta tatggatttg caggaatgaa aataaaaaat gataaattaa
     3901 gttttgaacc aaggatacct aaacactgga aaaatattaa atttaatata ttatttagag
     3961 ataatttagt ttttgtaaat atagaagaat ataaatctta ttataagtta atcaaaggta
     4021 gaaatataac catattacat gataataaag aaataaaatt atcaaagaac ataatagaag
     4081 ttaataattg ttttaaaggg agtaattaat atgaaagcag tcttatttga tcttgatgga
     4141 gttataacag atactgctaa gtatcatttt gaagcttggc atgagatagc aaaatcgcta
     4201 gatatagaaa tagatgaaac ttttaatgaa aaattaaaag gcataaacag ggaagaatca
     4261 ttagataaaa tattgttatc tgctaataaa gtagatagtt atactaaaga ggaaaagtta
     4321 aaggtgtgtg aagaaaaaaa taacatttat ataaagttaa taaactcatt aacatataaa
     4381 gatatattac cagggataaa tgagcttata aaagaactta aaaaaaataa tgtgaaattg
     4441 ggaatagcat ctgctagtaa gaatgcacct aggatattag aatctttggg agtaaaagat
     4501 gaatttgatt gtatagtaaa tccatcttta ttaaaaaggg gaaaaccata tcctgatatt
     4561 tttattgaag ggagcaagat gctaaattta aaacaatatg attgtgtagg aatagaagat
     4621 tcttattcgg gtattaaagc tataaatgct gccaatatga catcaattgg tgtggggaaa
     4681 aaagaagtac taggaaaatg tgaccatatt gtagaagata ctagcttatt aaattacaat
     4741 acactattac aagcttggag taaaaagatt aatttggatt aaaattatat taaaaattat
     4801 tttaaaagag aggataaggt atgaagaata aatatgaaga tgtagctcaa ttgataattg
     4861 atggacttgg aggaaaagat aatataattg acttaactca ttgtatgaca cgattaaggt
     4921 tcatcttaaa agatgaaaag aaagtcaatg cagataaatt aaaatctata gataaggtag
     4981 ttggagtaaa ctcaacttca actcaatatc aggtcataat aggtaatgag gtaggtgttg
     5041 tatttaatgc aataataaat aaggggataa ataccacagg agatattaaa agcgatataa
     5101 aaaataaaaa taaaaaaaat agtattttaa ataatataat agatacaata actggttgta
     5161 tgacacctat gataccagcc cttacagcat ctgggatgat aaaagttata ttatctcttt
     5221 gcaccacttt taattggcta tcatcaaatt caagtacata tagagtatta gatataatag
     5281 gagatgtagc attttacttt atgcctataa tacttgcaat aaatgcagct aaaaaattta
     5341 atgttaatac atcaatagca gttatagtag taggtgtgtt tttacatcca aatttctctg
     5401 catgggtatc aagtggggac cctatatcat ttataggagt accaattcaa ggtgttatat
     5461 atgcagcatc tgttatccct gcacttttaa cagtatggat gatgtcatat atagaaaatt
     5521 tcattgataa attaacacct agcatgttaa aaacaatttt aaatccaact ttagtattat
     5581 taataagtgc gccaatagca ttaatagtaa ttggaccaat aggaaatttc ttaggagagg
     5641 gattagctag tataataaat ttactacaag gaaggttagg atttataatg gtttgtttat
     5701 tagcagctgc aatgccattt atcgttatga cagggatgca tcatgcattg actcctatat
     5761 tcatatcagc atttgcaact acaggacaag aatcgctaat attaatatct caagtttgtg
     5821 ctaatttagc gcaaggtggc gcaacattag ctgtagcatt aagaagcaaa gatagtaaga
     5881 tgaaacagtt agcatcagct gcatggatat cagctattat gggtataact gaaccagctc
     5941 tttatggagt tacattgaaa cttaaaaaac ctgctatgtt agcatctata ggagcaggaa
     6001 tcgcagggtt atttgcagga attactcatg tgactttata tgttccacaa aatagtataa
     6061 tggcagtact tggattatca ggggaaaagg ggatttcaaa tatagtaaat ggacttatag
     6121 ttatatttat agcattggtt gctccattta ttatgacata tgtttttgga gttgaaaaaa
     6181 atgttaaata aagtacagat aatgtagtag aggaagtata atattaacta tttacattaa
     6241 tagaattttt attattattg actataactt gttttgtatt tatattgatg caaagaaata
     6301 agttaatatt aataagaata attattataa ctgacaaaaa gcaacataaa agattgatga
     6361 agtatatgga aataaaaagt tgacaaaata tgtctgatgt tttatcatat taattggaat
     6421 taggtagtaa ttgtttaagt acaagctaag cttattcatt aataatagca gtatcctagg
     6481 aaggagtatg cccattatta tggataagct ttttttatgc ttaaaaggag gataatcatg
     6541 ataattaata agatattaaa caataatgtt gtaatcacat tagatgataa tgatgaagaa
     6601 gttatcgtta tgggtaaagg aattggttat caaaaatcaa aaggcaatct aatagataaa
     6661 actaaagtaa ataaagtatt tagaatatca aataaagaaa tatcaaacaa actacaagaa
     6721 ttactcaata atattcctat agaacacatg aaattatcaa gtgaaattat agaatacgca
     6781 caaataaagt taaacaagaa gctaaatgaa agtatttaca tatccttaag tgaccataca
     6841 tattctgcaa ttcaaagaat gaaagaagga ataaatgtaa aaaatgcaat cctttgggaa
     6901 attaaaagat tttataaaga agagtttgaa attggaatga aagcactaga tattattgaa
     6961 aacaaaacag gtataaaatt accagaagat gaggctggat ttatagcctt tcatatagtg
     7021 aatgctcagt taagtgaagg tcatactttg gcatctgata ttacgaagtt aatacaagaa
     7081 gtacttagca tagttaggta tcattttagg atagaatttc atgaagaatc agtgttttat
     7141 tatagattta taatgcattt aaagtttttt gcgcaaagat tgcttttaga tagtgcacat
     7201 gaaggtgaga cagacaaaga attactaagc attattaaat ctaaatataa taaagagttt
     7261 gaatgtgtag ttaaaattaa aaatttcatt aaaaaacaat ataactacat attaacagat
     7321 gatgagctta tatatttaac aatacattta gctaaagtag ttaaagactc aaatatgtaa
     7381 gtgaaataat ttttcagcag tatattaaga atgattgaaa ggagtgatac taacaaaatt
     7441 atacttatgg ttggatggta acattgagtt ggccaaacct tcaaatttca aaaaattaat
     7501 ttaaacgaaa tatggaggag aaaaatggga aaatacaaac aattagcaca agagatagta
     7561 aacaatgttg gtggaaaaga aaatattagt ggattagtac actgtattac aagattacgt
     7621 tttactttaa aagatgaaag tatagcaaat gacaatgtat taaaaaacat ggaaggtatt
     7681 gttactgtaa tgaaaagtgg aggacaatat caagtagtta taggaaatca tgttgaagct
     7741 gtctataaag atgtagttga aataattggt ttagatgatt caagtacaag ttcagaaact
     7801 aaaaaatcag gtaatatact agataaaggt atagatataa tatcaggaat ttttcagcca
     7861 gtgttaggaa taatggcagc ttgtggtatg ttgaaaggat ttaatgcatt atttgtagca
     7921 atgggattat atagtgatgt atctggtggt tatatggtaa taaatgcagc gggagatgca
     7981 ttatttacat tccttccttt gttcttagga tatacttcag ctaagaagtt tggattaaaa
     8041 ccaatgttgg gattagcact aggagctgca gtatgttatc ctgctataca aggtagcagt
     8101 atatcagcag gagcagatgt gatgtacact ttatttaaag gaacaatgtt tgcatcacca
     8161 gtatatattg atttctttgg actaccaatt gtatcaattg attatactgg aacagtcgta
     8221 ccaattatat ttatagtgta ttttgcttct aggtgtgaaa aattatttaa taagtgtgtt
     8281 ccaaatttag ttaagttttt ctttgtgcca atgcttacat tattaataac agtgcctgtt
     8341 gcacttatag ttataggccc tatagcaaca tttggttcta cacttatttc acaaattgta
     8401 atttcaataa gagattttag tccactattg gcaggtgcta tagtaggatt tacttggcaa
     8461 attcttgtta tttttggtat gcactgggga tttattcctg tatatataaa caatgttatg
     8521 actttaggat atgataatgt tatgatgcca ttctttgcct gtacatttgc aagttcggca
     8581 gttgtgttgg caatattttt caagactaaa gataaaaaac taaaagagat ggcaatacca
     8641 aactttatat ctggtatatt tggagttaca gagccagcaa tttatggaat attattgcct
     8701 ttaaagaaac catttataat aagttgtatt gctagtggta ttggaggcgc tttctatgga
     8761 ttctttaatc ttagaaaatt tataactgga ggaatgggaa tatttgaact tccagcaatg
     8821 attgaaccag atgggggaat gggtaactta atagtagctc tttctggtat agctatatca
     8881 atggtcgtag cttttgtatt aactatgatt ttatataaag atgagaaagt agaagagtct
     8941 aaaaaagtaa gaaataaggg taaagaagaa ataaaagaag tcaagtctac taaattagaa
     9001 agagaaatag taacaagtcc tataaaaggt gaagtattaa aattaagtga tatagaggat
     9061 gcagcatttg catctggtgt actaggtcaa ggggtggcaa taatacctag tgatggaaaa
     9121 gtagttgctc cagtagatgg ggtagttaca accttatttc ctagtctaca tgctataggg
     9181 attctttctg atacaggtgt tgaagtgctt attcatattg gattaaatac tattcaatta
     9241 gagggaagag gttttgaggc atgtataaaa caaggtgata gaattacaaa aggtcaaact
     9301 atattaaact ttgatgttga tgcaataaaa gagttggggt atagtactgt aactccaatt
     9361 gttattacaa attcatcaca atttttagat gtagtagaaa cagaaagtaa aaatattgaa
     9421 ttagaagaca atctaattac agtcttattt tagtaaagat tgaggtatta gcataatcta
     9481 gtgctttggt tataatttta ggaggctttg actatgagtt ttaagaatga ttttttatgg
     9541 ggaggagcta ctgcggccaa tcaatgtgaa ggtggataca atgaagacga aagaggattg
     9601 gcaaatgtag atgtatgtcc aacaggaaaa gatagaacag cagttattac agggaaacta
     9661 aaaatgtttg attttgatga tgagcattat tatccagcta aaactggtat tgatatgtat
     9721 cataattata aagaagatat aaagttattt gctgaaatgg gatttaaagt atatagaatg
     9781 agcattgcat ggagtcgaat atttccaaaa ggtgatgaag aaacaccaaa tgaaaaggga
     9841 cttcaatttt atgaaaatat atttaaggag tgtaaaaaat ataaaataga gcctttagtc
     9901 actattacac actttgattg tccaatgcat ttagttaaaa agtatggtgc atggcgtaat
     9961 cgaaaaatga taggtttcta tgagaaatta tgcaatgtta tctttagaag atataaagga
    10021 ttagtgaagt actggctaac tttcaatgaa ataaatatga ttttacatgc tccatttatg
    10081 ggtgctggta tttgctttga agaaggtgaa gatgttgaaa agataaaata tcaagctgcc
    10141 catcatgagc ttgtagctag tgcaattgca actaaaatag cacatgaagt tgattcaaat
    10201 aacttaattg gatgtatgtt tgcagctgga agtgtatatc cttatagttg taatcctaat
    10261 gatgtatggg aagctacaaa gttagataga gaaaattact tctttgtgga tgtacaatct
    10321 aaaggtaaat atcctaacta tgcgttaaag tatatggagc aaaaaggtat tacaccagaa
    10381 atggaacctg gtgatattga attattgaat aaatatacag tagattttat ttcattttca
    10441 tattacaata gccgttgtgt tagaaccgat gaaaatgcag atgatatggc tgaaggaaac
    10501 atatttacat ctgctaaaaa tccatatctt tcatacagtc aatggggatg gccaattgac
    10561 ccattaggtt tgagaattac actaaatcat gtatatgata gatatgaaaa gccactattt
    10621 gttgtagaaa atggattagg tgctaaagat atagctgatg aaaatggtta tgtagaagat
    10681 gattacagaa ttgattatat aagagaacat ataaaagcta tgcatgatgc agtttctata
    10741 gatggggtcg atttattagg ttatactaca tgggcaccta ttgatttagt aagtgctggt
    10801 acaggtgaaa tggaaaagcg ttatggattt atatatgtag atagagacaa tagtggtaat
    10861 ggaagtctaa gaagaatgaa aaagaaaagt tttgaatggt ataagaaagt aattgcttca
    10921 aatggtgaag atttatatta gacaagaaat ctatgttaaa taagaaattt gtattaaata
    10981 agaagagtat aaaaattaat aataagtata atttttaact tttttaacta tagaaaatca
    11041 atacagaatt attttacatt gtatttatgc aatagtatga ttcacaaata aaattttaga
    11101 aaataaaatt gttaagaagt atgggaagta agtgcaattt actttgtaat aaaagatgaa
    11161 aaaggcaagc ttaggtttta agcttgtctt ttttattatg tataaactat gatactttaa
    11221 aattctatga aaaatttatt taatagagaa attaaaaatt taatggtaat caaattcttt
    11281 accaatgaat ataaaaaatg tctacgtgtt ataatagttt aaactaataa acgaaaggta
    11341 gatgaatgaa tatgaaatac caacatataa tagaagttga caaagaactt tgcattggat
    11401 gtgggctttg taaaaatgat tgtccagtaa acaatataat tatagaaaat aaaaagtcag
    11461 taattaaaaa acaagattgt ctaatgtgtg gacattgtgc agcaatatgt cccacaaaag
    11521 ctattacctt gacaggattt gatgaacctc ctatagaact tacaaataag ccaaagctag
    11581 attcagatga attactaatg gcaattaaat ctagaagaag tatacgtaaa tttaaagaca
    11641 aagaagtttc ttcagaaatt ataaagcaaa ttattgaggc aggtaggtat acccctagtg
    11701 ctaaaaattc acaagatgta tcatatattg ttttagacaa taaaaagtca atttatgaaa
    11761 acgaagcagt gaaatttttt agaaaaataa aacctattgc aaatatagct attaaatatt
    11821 caaaagaagt tgagattgac gataactttt tctttaagca tgctcctatt gcaataatga
    11881 taattacaaa agataagatt agtggttcac ttgcagcatc aaatatggca ttaatggcag
    11941 agtcttatgg cctaggtgtt ttatttagtg gatatttttc agatgtagca aataattcgc
    12001 caaaattaaa aaagctttta ggtctaaaac gcagtaatca tgttcttaca acattagtaa
    12061 ttggataccc agatgttaaa tatagacgta ctgcacagaa agaagtagca actgttagat
    12121 acttataaga gagggtggat tttatggaaa aagaatgtca acaaagaatg caagaaataa
    12181 cgtctaaatt taaagaatgt cgcaaagcta tctctgctat gggtgatgag actagacaat
    12241 tgattttaat tgctctctta gagagtgatt ttaatggtat acgagtggga gaaatcacaa
    12301 aaaaaacaca tctttctcgt cctgctgtat ctcatcatct taagatatta aaagaagcag
    12361 agattgttaa tttgcgtaga gaaggaacaa aaaattatta ttatcttgat tcaaaaaaat
    12421 cacaatggag gtcactaaca gagctaataa atcttatatt tgttggaata caacatatta
    12481 gtgaaaatga tataagaaaa ggtgattaaa ttatacttga tttaatacaa aaagattgat
    12541 ttaggaacta aaaagaacta aaaagttcac tttgagacac tatcttttga actataccca
    12601 tcttaggaga cagttaaaat atgtaaaact gtataaaaca atgggtatag ttcattttat
    12661 aatggtttaa atattaagaa tatattattc taaatagtat ctttctctgt tttgagtttt
    12721 atttttatac tcaatagctt cagtggggca accacaaata catgccatac aatgagtaca
    12781 gttatttttc caaactggtt ttttgttttt taaattaata ttatttaaag gacatagctc
    12841 tacacatttt ccacagccaa tgcatttatc agtataatga aatcctttgg cactaataat
    12901 tgtcttataa aaaactgtat ttattatacc acttttaatt gtacctccta agccactagg
    12961 agtaataatt tcaaattcat tttcatttcg tatatcagaa ataatcctat atatttgttt
    13021 atctgcttct ttaatcattt gttttgatgt ttctttgtct gttgtagaaa ataaagctat
    13081 ataattctct ggcattttaa tttctgccat accttttaat tgccattcct tgtatttaca
    13141 tagtttttga atatagttta tggattttgc tgtatctcca ccacaagtca ttacaaagta
    13201 aactctgttg cttcctagaa attttgtttc ttttataaaa tccgtaacca ctattggcaa
    13261 tctccatgca taagtaggac atacaaatat gaatggttta tctgttgata ttaattcatc
    13321 tgttttttcc tcttttataa gttgatttag tgatatcagt tcatcattta attcttgtgc
    13381 aattttacga gccacatatc ggctattacc agttccgcta aaatataaaa tcataataat
    13441 atctccctaa ctatttttta ttttaactct taatttttta tttattatag taatttattg
    13501 atatattcta ttcaaatgta tgtaaaattt agtagttaag aaaattataa cacattagta
    13561 atttttatac ttattggtaa agaatttgat taccattaaa tttagcaaca agacatcaac
    13621 tggacttgta tatgagaagg gataataatt tgcttatgaa agattataaa tataaaggtt
    13681 attttgaagg tgtagaaata tttgataatg taaatgacct taacttaaat actttaacaa
    13741 cagttagtag atttgtgttt gtttttttgt ttacgataac ttaaggaaat atgttacctg
    13801 aacaataaat ttatataata aaagtatata aatatatata tgaatgttaa gattagttga
    13861 tagaatttaa ataataaatc caatattata aacataggag gtatgaaaaa tatgattaat
    13921 gagaatttaa aaagtctaag aaaaatcaat cagtatacac aagaagaatt agctgaaaaa
    13981 cttaatgttt ctagacaatc aatagcaaaa tgggaaagtg gagaatctat tcctgatata
    14041 ggtagttgca ttaaacttgc aaaattgtac aatgttaaac tagatgactt agttaatcat
    14101 tcggaagaaa aaacaggtat tatagtacct cctaaaggaa agtttttctt tggagcagtt
    14161 gtagttggtg aacgtggtca gattgtcatt ccaaaagaag caagagaagt atttaatata
    14221 aatgcaggag ataaattatt ggtacttgga gatgaggaaa gaggattagg aattgttcat
    14281 caacgtgatt tgataaattt tataggtcat gtgggagtag tacataaaga agaatgattt
    14341 aaaaaaatta taaaaggagg tatgaaatat gagtaaaata gtatttttta gcattcctgc
    14401 atatggtcat actaatccta ctattgaagt agttcgtgaa ttagtggata gaggaaatga
    14461 ggtattgtat tattctttta atgaatttaa agataaaatt gaaggtgctg gagcaaaatt
    14521 tatttgttgt gataaatacc ttccagaatt actccctgga gatgagaaaa aaattggtaa
    14581 ggattttcct ggattaattg agatgatagt agatactact atttctttag atgaaaaagt
    14641 ttgtagagaa cttaaagatt ttaatccaga ttgcattgta tcagattcat taagtttttg
    14701 gggaaagtta tttgcacaaa aacttaatat tccatatgta tgctctacaa caacttttgc
    14761 atttaataaa catacagcaa agttgatgaa acaaaacttt atagaaatta tacgtatgat
    14821 ttttggtgta agacgcatta ataaaaaaat aaagctttta caaagaaaag ggtatgaagt
    14881 aaaaaatttt atttctatag tttctaatga taatgatacg gataccattg tatatacatc
    14941 aaaagaattt caaccaatgg tagaaacctt ctctagtaag tattcatttg taggtccatc
    15001 agtatcaaag ttgattattg aacctaaaga gagaaaaaga aagctaatat atatttcgct
    15061 tggtacaatt aataataaaa atgttagctt ttataaaaac tgtataaatg catttaaaga
    15121 ttctgatgtt gatgtgatta tgtctgttgg tagaagtact gatattaaaa gtttaggaaa
    15181 tataccaaat aattttgagg ttaaaaatag tgttgaacag attgctatac ttcaaaaaac
    15241 tgatgtgttt gttactcatt ctgggatgaa tagtgttaat gaaagtttat attatggagt
    15301 acctatggta ttatttcctc aacattcgga gcagagaatg gtagctaaga gagttgttca
    15361 tttgggagca ggaattatgt taaaagagga taaatcagaa agtattaaaa aagcagtatt
    15421 tcaagttata aatgacaatg aatataaaga aaatgcaact aaactatcaa aaagctttta
    15481 taatgcagga ggctcaaaaa aagcagcaga tgtaatatta cagattatat gtaatagtag
    15541 acaagttgac atataataga tttgttaaaa atatatatac atacatgtaa tttaatcaaa
    15601 gtactgacag tggggtcttt taacctacca gttggggcat atatattgat gctaaatatg
    15661 agggatgtta tattttaata aaaaagataa catccttttt ttagtgcatt atatcgagtt
    15721 ttaaataata aatgaaggag gattaatatg aaaaaatata aagttatttt tatagtatta
    15781 attataggta ttataagtgt aacagggttt gttgtatata aaaactgcat taaaccatgg
    15841 aaagatgctg aaacatatat tgataagtat atggtaaaac aagatatttc aaaagataat
    15901 ataaaaagta ttacaaaaga aaaagccaag aaggcaagtt acgaaggaat actatacaaa
    15961 gtgtattaca aagatgaccc tcgatatgag tatcaatatt tttatagtga taattattat
    16021 gctctatatg taaataaagt aatgttacaa atttatgata tggaaaataa taataaactt
    16081 ttaagaagta atgaggaatt aaaaagtgta aagtatccac caatatatct taaatataag
    16141 ccagataaat gatttatgac aaaatagaat aatttttatt aaaatttatt taaaaatatc
    16201 actagagcgt ttaaataaaa ctaaatttaa gtatttatat attagtatat cttactagtg
    16261 tttaaacaaa a
//
LOCUS       DAYAPA010000095        15978 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.095, whole genome shotgun sequence.
ACCESSION   DAYAPA010000095 DAYAPA010000000
VERSION     DAYAPA010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15978
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.095"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            248..397
                     /gene="rpmG"
                     /locus_tag="ACGI59_002769"
     CDS             248..397
                     /gene="rpmG"
                     /locus_tag="ACGI59_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014262850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFG9740548.1"
                     /translation="MRVKVTLACTECKQRNYNTTKNKKNNPDRIELQKYCRFCKKHTT
                     HKETK"
     gene            423..644
                     /gene="secE"
                     /locus_tag="ACGI59_002770"
     CDS             423..644
                     /gene="secE"
                     /locus_tag="ACGI59_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFG9740549.1"
                     /translation="MAAQKNDGAKTKKRFSLFGYLKETKQELKRVTWPTKKELFKNTS
                     IVLTVVISCTILVWGIDTILSGALALLLK"
     gene            664..1206
                     /gene="nusG"
                     /locus_tag="ACGI59_002771"
     CDS             664..1206
                     /gene="nusG"
                     /locus_tag="ACGI59_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFG9740550.1"
                     /translation="MSELQEASWYVVHTYSGHENKVKATIEKAVKTRGMEDCIRQVVV
                     PTEEVVETTKTGKEKTRQRKVYPSYVLVKMIITDESWYVVRNTKGVTGFVGPGSKPVP
                     LSEDEVKAMGIDTTDPKVVNSDIDFEIGDTVKVSQGPFSGQIGNIEEIDLENREVKVC
                     INAFGKRTLFVIELEGIEKI"
     gene            1245..1670
                     /gene="rplK"
                     /locus_tag="ACGI59_002772"
     CDS             1245..1670
                     /gene="rplK"
                     /locus_tag="ACGI59_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFG9740551.1"
                     /translation="MAKKVIGQIKLQIPAGKATPAPPVGPALGQHGVNIMGFTKEFNA
                     KTADQAGMIIPVVITVYQDRSFSFITKTPPAAVLIKKALNLKSGSGEPNKKKVAKMTS
                     AQVREIAELKMPDLNAASVEAAMSMIAGTARSMGVVIED"
     gene            1741..2439
                     /gene="rplA"
                     /locus_tag="ACGI59_002773"
     CDS             1741..2439
                     /gene="rplA"
                     /locus_tag="ACGI59_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFG9740552.1"
                     /translation="MAKKGKRYAGALQKVDRTKFYDASEALTLVSDIAGAKFDETVEA
                     HIKLGVDSRHADQQVRGAVVLPHGTGKTKRVLVFAKGEKAKEAEQAGADFVGAEELVQ
                     KIQGENWFDFDIVVATPDMMGVVGRLGRVLGPKGLMPNPKSGTVTFDVAKAIDEIKAG
                     KVEYRLDKTNIIHVPVGKVSFGGEKLTENFTALMDAIIKAKPAAAKGQYLRSITVASS
                     MGPGVKINPARTAE"
     misc_feature    2491..2619
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            2662..3168
                     /gene="rplJ"
                     /locus_tag="ACGI59_002774"
     CDS             2662..3168
                     /gene="rplJ"
                     /locus_tag="ACGI59_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFG9740553.1"
                     /translation="MRKAIEIKSEVVSEIVEKLQKSSAAVVVDYKGLTVEEVTELRKQ
                     MREAGVDYKVYKNTLVRRAAKEVGIEQFNDELLVGTNAIAFGYDDPVAPARILKGFMD
                     SHPKMKLKMGIVEGAFYDESKIVEMANIPSREVLIAKLLGSLKAPVSNFAYLIDAIAK
                     KAEGQEEA"
     gene            3226..3591
                     /gene="rplL"
                     /locus_tag="ACGI59_002775"
     CDS             3226..3591
                     /gene="rplL"
                     /locus_tag="ACGI59_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002842856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFG9740554.1"
                     /translation="MTIEQILEAIENMKVLELNELVKAAEEKFGVSASAPVMVAGAAA
                     GGPAAEEKTEFDVVLTDVGSSKVGVIKAVREITGLGLKEAKEVVDNAPKTVKEGASKE
                     EADQIKEKLEAAGAKVEVK"
     gene            3982..4770
                     /locus_tag="ACGI59_002776"
     CDS             3982..4770
                     /locus_tag="ACGI59_002776"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFG9740555.1"
                     /translation="MEKLQGKIAVVTAATKGIGLASAEILAKNGATVYLAARSEELAN
                     EVINKISAEGGCAKFVYFNAREEETFTSMIEEVVKKEGKIDILVNNFGSTNPSLDKDL
                     VTGDTDNFFDTVNTNLKSVYLPCKAAIPHMIKNGKGSIVNISSIGSVLPDLSRIAYCV
                     SKAAINSLTQNIATQYAKDNVRCNAVLPGLIATKAALDNMSPEFIKEFLKHVPLNRIG
                     EPDDIAKAVLFYASDDSSFITGDLLEVAGGFGLPTPQFADNILG"
     gene            5241..8957
                     /gene="rpoB"
                     /locus_tag="ACGI59_002777"
     CDS             5241..8957
                     /gene="rpoB"
                     /locus_tag="ACGI59_002777"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFG9740556.1"
                     /translation="MPHPVTIGKRTRMSFSKIKEIADVPNLIEIQVDSYEWFLKEGLK
                     EVFDDISPIEDYTGNLILEFVDYSLDDKPKYDIEECKERDATYCAPLKVKVRLINKET
                     GEIKEQEVFMGDFPLMTERGTFVINGAERVIVSQLVRSPGVYYAEERDKTGKRLISST
                     VIPNRGAWLEYETDSNDVISVRVDRTRKQPVTVLLRALGIGTDAEIIDLLGEDERLSA
                     TLEKDNTKTVEEGLVEIYKKLRPGEPPTVESASSLLNALFFDPKRYDLAKVGRYKFNK
                     KLALCYRIMNKISAEDIINPETGEVFVKAGEKISYDLAKAIQNAGINVVNLLMDDDKK
                     VRVIGNNFVDIKSHIDFDIDDLNIKEKVHYPTLKEILDGYSDEEEIKEAIKSRIKELI
                     PKHILLDDIIASISYEFNIFYNIGNIDDIDHLGNRRIRSVGELLQNQVRIGLSRMERV
                     IKERMTVQDMEAITPQALVNIRPVSAAIKEFFGSSQLSQFMDQTNPLSELTHKRRLSA
                     LGPGGLSRERAGFEVRDVHHSHYGRMCPIETPEGPNIGLINSLGTYAKINEFGFIESP
                     YRKFDKETSTVTDEIHYLTADEEDLFVRAQANEPLTEDGKFVNHRVVCRTVNGAVEMV
                     PESRVDYMDISPKQVVSVATAMIPFLENDDANRALMGANMQRQAVPLVRREAPIIGTG
                     IEYRAAKDSGAVVVARNSGIAERVTADEIIIKREDGNRDRYNLLKFKRSNSGTCINQT
                     PIINKGDQIIKGDVIADGPATDLGEVALGRNCLIAFMTWEGYNYEDAILINERLVKED
                     RLSTIHIEEYECEARDTKLGPEEITRDIPNVGDSAIKNLDDRGIIRIGAEVDSGDILV
                     GKVTPKGETELTAEERLLRAIFGEKAREVRDTSLKVPHGESGIIVDVKVFTRENGDDL
                     SPGVNELVRCYIAKKRKIKVGDKMAGRHGNKGVISRVLPEEDMPFMENGTPLDIILNP
                     QGIPSRMNIGQVLEVHLGLAAKTLGWYVATSVFDGANEYDIMDALEEAGYPRDGKLTL
                     YDGRTGESFDNRITVGYMYYLKLHHLVDEKLHARSTGPYSLVTQQPLGGKAQFGGQRF
                     GEMEVWALEAYGAAHILQEILTVKSDDVVGRVRTYEAIVKGENIPEPGIPESFKVLIK
                     ELQSLCLDVKVLTDEDQEIEVRESVDEDDTIGEFELDVVNHMGEVEESNIIEEIEDDF
                     AENAEDEDIENLEEFTEDDLFEEEIDFDSDDFDM"
     gene            8999..12484
                     /gene="rpoC"
                     /locus_tag="ACGI59_002778"
     CDS             8999..12484
                     /gene="rpoC"
                     /locus_tag="ACGI59_002778"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFG9740557.1"
                     /translation="MFELNNFESIKIALASPEKIRQWSRGEVKKPETINYRTLKPEKD
                     GLFCERIFGPQKDWECHCGKYRRVRYKGVVCDRCGVEVTKSKVRRERMGHIELAAPMS
                     HIWYFKGIPSRMGLLLDMSPRSLEKILYFASYVVVDPGETGLNEKQLLTEKEYRTALE
                     KYGYTFTVGMGAEAVKTLLQNIDLEQQSKDLRAELKDSTGQKKVRTIRRLEVVEAFKK
                     SGNKPEWMILDAIPVIPPDLRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLLELGA
                     PDIIVRNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSDMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPELKFYQCGLPKKMALELFKPFVMDKLVKEGYAHNIKSAKSIVEK
                     VKPEVWDVLEDVIKSHPVLLNRAPTLHRLGIQAFEPILVEGKAIKLHPLVCTAYNADF
                     DGDQMAVHVPLSVEAQAEARFLMLSVNNILAPKDGSPITTPSQDMVLGCYYLTIEAQD
                     GAKGTGMVFKDFNELLLAYYNKSVHLHALVKLKVTLEDGRSSLVESTVGRFIFNENIP
                     QDLGFVDRKENPFALEVDFLADKKSLGKIIDKCFRKHGNTETAELLDYIKALGFKYST
                     LGGITVAVDDMSVPEEKKVFIAEAEAKVDKYEKAYRRGLISDEERYEKVIETWTETTD
                     KVTDALMGGLDRLNNIYIMAHSGARGSKNQIRQLAGMRGLMANASGKTVEIPVKSNFR
                     EGLSVLEYFTSSHGARKGLADTAIRTAESGYLTRRLVDVSQDVIVREIDCGTEDTTEI
                     YAIKEGNEVIEEIYDRIVGRYTIDPILNPETGEVIVEADSMIQEDEAETIVALGIEKI
                     RIRTVLNCKTNHGVCSKCYGRNLATGKEVNIGEAVGIIAAQSIGEPGTQLTMRTFHTG
                     GVAGADITQGLPRVEELFEARKPKGLAVITEVSGRVEIDETGKRKEVNVIPEEGETQT
                     YVIPYGSRLKVKQGQMLEAGDPLTQGFINPHDIVRVNGVKGVQEYIVKEVQRVYRLQG
                     VDVNDKHIEVIVRQMLSKVKVEDPGDTDLLPGGYEDVLTFNECNKDAIDKGLRPAVAK
                     RVLLGITKASLATDSFLSAASFQETTRVLTEAAIKGKEDHLIGLKENVILGKLIPAGT
                     GMKKYRNIAVEKIED"
     gene            12773..13195
                     /gene="rpsL"
                     /locus_tag="ACGI59_002779"
     CDS             12773..13195
                     /gene="rpsL"
                     /locus_tag="ACGI59_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFG9740558.1"
                     /translation="MPTINQLVRKSRKALEKKSTAPALQKGYNSLNKKVTDASAPQKR
                     GVCTSVKTVTPKKPNSALRKVARVRLTNGIEVSAYIPGEGHNLQEHSVVLIRGGRVKD
                     LPGVRYHILRGTLDTAGVDKRRQSRSKYGAKRPKEAKK"
     gene            13319..13789
                     /gene="rpsG"
                     /locus_tag="ACGI59_002780"
     CDS             13319..13789
                     /gene="rpsG"
                     /locus_tag="ACGI59_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013054923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFG9740559.1"
                     /translation="MPRKGNIPKREVLPDPMYGSKVVTKLINNLMVDGKKGKSQRIVY
                     DAFAIVAEKTGEEALEVFNKAMDNIMPVLEVKARRVGGANYQVPIEVRPERRQTLGLR
                     WLVKYTRARGEKGMVEKLAKEIMDAANNTGASVKKKEDTHKMAEANKAFAHYRW"
     gene            13838..15904
                     /gene="fusA"
                     /locus_tag="ACGI59_002781"
     CDS             13838..15904
                     /gene="fusA"
                     /locus_tag="ACGI59_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFG9740560.1"
                     /translation="MARKFPLERTRNIGIMAHIDAGKTTTTERILFYTGQTHKIGETH
                     EGASQMDWMEQEKERGITITSAATTASWKDHRINIIDTPGHVDFTVEVERSLRVLDGS
                     VAVFCAKGGVEPQSENVWRQAETYGVPRIAFVNKMDILGADFYNVVSMMKSRLNSNAV
                     PMQLPIGKEDSFIGIIDLLKMDAVIYKDDLGVEMEETDIPEDMKELAAEWREKLVESV
                     AETDEELMMKYLEGEELTIDELKVAIRKATIACEMNPVFCGTAYRNKGVQLVIDAVLD
                     YLPAPTDIPAIKGILADGEEAERHSSDEEPFSALAFKIMTDPFVGKLAFFRVYSGTLE
                     SGSYVLNATKNKRERIGRILQMHANTREEITKVYAGDIAAAVGLKDTTTGDTLCDPAN
                     PIILESMEFPEPVISVAIEPSSKAAQEKMGIALQKLAEEDPTFTVKTDQETGQTIISG
                     MGELHLEIIVDRLLREFKVEAKVGAPQVAYRETITQPVDVEYKYSKQSGGRGQYGHVK
                     IRVAPQEPGEGYKFTNKTVGGSVPKEYVGPVDMGIQGAMQSGIVAGYPVVDVAVELYD
                     GSYHEVDSSEMAFKMAGSMAFKDAMKKGNAVLLEPYFKVEVVTPEDYMGDVMGDLNSR
                     RGLIQGMEARSGAQVINAFVPLSEMFGYSTDLRSSTQGRATYTMIFDHYEQVPASVAK
                     KIAEGK"
BASE COUNT         6257 a         1989 c         3249 g         4483 t
ORIGIN      
        1 ctttactaaa ctgatggatt aaaaggaata acaaaaagag gggttatgct cctctttttt
       61 taacatagaa atacaaaaac attgacacag cataggattt tgtgataact tatacaaggt
      121 aatgattata ggaagttaat aatatcttga atactgtaga tttttaataa atacatattt
      181 taaagatatt atattcacat atattaacaa tatatattat tgtattacaa agttggaggt
      241 gtatcagatg agagttaaag taactttagc atgtacagag tgtaagcaaa gaaactacaa
      301 cactactaag aataagaaaa ataatccaga cagaatagaa ttacaaaaat attgtagatt
      361 ctgtaaaaag catacaactc ataaagaaac aaaatagttt tttataagga tgtgagtatg
      421 gaatggctgc ccaaaaaaat gacggtgcaa aaactaaaaa aaggtttagt ttgtttggat
      481 acttaaaaga aactaaacaa gaattaaaaa gagttacatg gccgacaaaa aaggaactgt
      541 ttaaaaacac aagtatagtt ttaactgtag ttatttcatg cactatatta gtatggggta
      601 tagacactat attatctggt gcactagcgt tattactaaa atagacgaaa agaggtaagt
      661 taaatgtcag aattacaaga agcaagttgg tatgtagttc atacctattc gggacatgaa
      721 aataaagtta aagctacaat tgagaaagca gttaaaacaa gaggtatgga agactgcata
      781 agacaagtag ttgttccaac agaagaagta gtggaaacta ctaaaactgg aaaagaaaaa
      841 actagacaac gtaaagttta tccaagttat gttttggtta aaatgataat aactgatgaa
      901 tcttggtatg tagtaagaaa tactaaggga gttacaggtt tcgttggacc tggttcaaaa
      961 ccagtgccat taagtgaaga cgaagtcaaa gctatgggga tagacacaac agaccctaaa
     1021 gtagtaaata gcgatattga ttttgaaata ggagatacag taaaagtatc tcaaggacca
     1081 tttagtggac aaataggtaa catagaagaa attgacttag aaaatagaga agtcaaagta
     1141 tgcataaatg catttggtaa aaggactcta tttgtaatag agcttgaagg tatagaaaaa
     1201 atctaatact atattataga aaaatctgag gaggtgcgct caaaatggct aaaaaagtta
     1261 taggtcaaat aaaattacaa atacctgcag gaaaggctac tccagcgcca ccagttggac
     1321 cagcattagg acaacatggt gttaatataa tgggattcac aaaagaattt aatgctaaaa
     1381 ctgcagatca agctggaatg ataataccag ttgttataac tgtatatcaa gatagatcat
     1441 tcagttttat aacaaaaact ccaccagctg cagttttaat taaaaaagca ttaaacttaa
     1501 aatcaggttc tggagaacca aacaagaaaa aagttgctaa aatgacttca gctcaagtaa
     1561 gagaaatagc tgaattaaag atgcctgact taaatgctgc atcagtagaa gctgctatga
     1621 gtatgatagc tggtactgct agaagcatgg gtgttgtaat cgaagactaa ttaaaagata
     1681 cacttttttt tataaagtgg gaggctagaa gccgaaatta accacgagga ggaaaataaa
     1741 atggctaaaa aaggtaaaag atatgcaggt gcattacaaa aagtagatag aactaaattt
     1801 tatgatgcat cagaagcatt aacattagtt tcagatatag ctggagctaa atttgatgag
     1861 acagtagaag cacacattaa attaggtgtt gactcaagac atgctgacca acaagtaaga
     1921 ggtgcagttg tattacctca tggaactggt aaaacaaaaa gagttttagt ttttgctaaa
     1981 ggtgaaaaag ctaaagaagc tgaacaagct ggtgctgact ttgtaggagc tgaagaatta
     2041 gttcaaaaaa tacaaggtga aaactggttt gactttgata tagtagttgc tactccagat
     2101 atgatgggtg tagtaggtag attaggtaga gtattaggac ctaaaggttt aatgccaaac
     2161 cctaaatcag gaacagttac atttgatgta gctaaagcta tagatgaaat aaaagctggt
     2221 aaagttgaat acagattaga caaaactaac ataatacacg ttccagtagg aaaagtatca
     2281 tttggtggag aaaaattaac tgaaaacttt actgcattaa tggatgctat aataaaagct
     2341 aaaccagctg ctgctaaagg acaatattta agaagtataa cagtagcttc ttcaatggga
     2401 cctggagtta aaataaaccc agcaagaaca gctgaataat aaaactgttg acacaggttg
     2461 acaaaaatgt tattatatca cttgtcaatg taaaaaagaa tagatttgcc gtagatagtg
     2521 ggtgcgaaag cttaatatcc cgccgaggtt ttagataaat tgagattact aaagcttttg
     2581 atgtctactt ttggcaacaa aagcttttta taatgttgcg gtaaatacta agcctatttg
     2641 gtgaaggagg tgcacatcta tatgagaaaa gctatagaaa ttaaatcaga agttgtttct
     2701 gagatagttg aaaagttaca aaaatcttct gctgctgttg ttgtagatta caaaggatta
     2761 acagttgaag aagtaactga gttaagaaaa caaatgagag aagctggtgt agattacaaa
     2821 gtatacaaaa atactttagt tagaagagca gctaaagaag ttggtataga gcaatttaat
     2881 gatgaattat tagttggaac taatgccata gcatttggat atgatgatcc agttgctcca
     2941 gctagaatat taaaaggatt catggattct catccaaaaa tgaaattaaa gatgggtatt
     3001 gtggaaggtg cattctatga tgaatctaag attgttgaaa tggctaacat accatcaaga
     3061 gaagttctta tcgctaaatt acttggtagc ttaaaagctc cagtatcaaa cttcgcatac
     3121 ttaatagatg caatagcgaa aaaggcagaa ggtcaagaag aagcttaatt gcttaactaa
     3181 aaaattataa atattaattt gaaataaaat tcggaggtgc taaaaatgac aatagaacaa
     3241 atattagaag ctatagaaaa tatgaaagtt ttagaattaa atgaattagt taaagctgct
     3301 gaagaaaaat tcggtgtatc agcttcagct ccagtaatgg tagctggtgc agctgctggt
     3361 ggaccagctg ctgaagaaaa aactgagttt gatgtagtat taactgacgt tggttcttca
     3421 aaagttggag ttataaaagc agttagagaa ataacaggat taggattaaa agaagctaaa
     3481 gaagtagttg acaatgctcc taagacagtt aaagaaggag cttctaaaga agaagctgat
     3541 caaataaaag agaaattaga agctgctgga gctaaagtag aagttaagta gtttctttaa
     3601 aaagataccc attgggtatc tttttttatt tataaaaaac tataaagaaa gtatttggaa
     3661 ttataaagaa agtattttta taaaataaaa aaaatttata taatagtact aaattaaggg
     3721 tatcatatat ggtgtaatgg tattttaatt taacattatt atcaaattaa aatataaaca
     3781 attgttgtaa tgtctgtata taaagtatat aattaaacta aaatgtaaaa ttttacattt
     3841 tagtttaaaa aaatacacga gtgtttataa ataaaatgta ataaataatt aattaacaaa
     3901 tataaatttt aaaataagtt agatatttag aaaaatcgaa atttgtagga tttaaaggac
     3961 atattcaagg aggagttaat aatggaaaaa ttacaaggaa aaattgcagt agttactgca
     4021 gcaacaaaag gtattggatt agcatcagca gagatattag caaaaaatgg agcaactgtg
     4081 tacttagcag ctcgttcaga agaattagct aatgaagtta taaataaaat aagtgcagaa
     4141 ggtggttgtg ctaagtttgt ttactttaat gctcgtgaag aagagacctt tacctcaatg
     4201 atagaagagg tagttaaaaa agaaggtaag atagatatac ttgtaaacaa ttttggttca
     4261 acaaaccctt ctcttgacaa agaccttgta actggggata cagataattt ctttgacaca
     4321 gtaaatacta atttaaaaag tgtgtattta ccatgtaaag cagcaattcc tcatatgata
     4381 aagaatggaa aaggaagtat agtaaatata tcaagtatag gttcagtatt acctgattta
     4441 tctagaatag cttactgtgt atcaaaagca gcaatcaact cattaactca aaacatagct
     4501 acacaatatg ctaaagacaa tgttagatgt aatgctgtac ttccagggct tattgcaact
     4561 aaagcagcat tagacaatat gtctccagag ttcataaaag aatttttaaa gcatgttcct
     4621 ttaaatcgta taggggaacc agatgatata gcaaaagctg ttctattcta tgctagtgat
     4681 gactcatcat ttataacagg ggatttactt gaagttgcag gaggatttgg tttacctact
     4741 ccacaatttg cagataatat attaggataa ttaaattact aatatgcaat gaaagcttat
     4801 gcattagtta tagtagaaat gatattttta taaaggccat agatataatt tatattggaa
     4861 aggtttgagg ttcaaaattc caagttaaat ttatttatgg tctttatttt gtatatttta
     4921 ataaattgta tatatttgta taaatgtaat tatttacaat cattttaata tggaattata
     4981 attaaatagg ggggatggta cttgtataaa attttttata gaaaattaga cctataaatg
     5041 aaaaatatgg attttatatt ggaaaattgt acataataca taatatatgc ttaaaataat
     5101 ttacaaaaaa tcaaaaataa tgcatagttt acatataaga taaaaaatat attgaaaaat
     5161 acttgacatt atgctaaaat tattagatgc gttagaatga actattgttt tatttttaat
     5221 tattgagagg tgaaaagtga atgccacatc ctgtcacgat aggtaaaaga actagaatga
     5281 gcttttctaa aattaaggaa atagctgatg tgccaaatct tatagaaatt caagtagatt
     5341 cctatgagtg gtttttaaaa gaaggtttaa aagaagtgtt tgatgatatt tcaccaatag
     5401 aagattatac tggtaatctt atattggaat ttgtcgacta ttctctagat gataaaccaa
     5461 agtacgatat agaagagtgt aaagagagag atgctacata ttgtgcacct ctaaaggtta
     5521 aggtaagact tataaataaa gaaacaggtg agataaaaga acaagaagtg tttatgggtg
     5581 acttcccttt aatgacagaa agaggaactt tcgttataaa tggagcagaa agagttatag
     5641 taagtcaatt agttagatct cctggtgttt actatgcgga agaaagagat aaaacaggta
     5701 agagattgat ttcatctact gtaattccta atagaggagc atggttagaa tatgaaacag
     5761 attctaatga tgtaatatct gtaagagttg atagaacaag aaaacaacca gttacagttt
     5821 tacttagagc tttaggaata ggaacagatg cagagataat tgacctttta ggagaagatg
     5881 agagattatc tgctacatta gaaaaagata atactaagac agtagaagaa ggtcttgtag
     5941 agatatacaa aaaattaaga ccaggtgaac ctcctactgt agaaagtgca tcatctttat
     6001 taaatgcttt attctttgac ccaaaaagat atgacctagc aaaagttggt agatataaat
     6061 tcaataagaa acttgcttta tgttatagaa taatgaataa aatttcagca gaagacataa
     6121 taaatccaga aactggagaa gtatttgtaa aagctggaga aaaaataagc tatgatttgg
     6181 ctaaagccat acaaaatgca ggtataaatg tagtaaatct actaatggat gatgataaaa
     6241 aagttagagt tataggaaat aattttgtgg atattaagtc tcacattgat tttgacatag
     6301 atgatttaaa cataaaagaa aaagtacatt atccaacttt aaaagaaatt ttagatggat
     6361 atagtgatga agaagaaata aaagaggcta taaaatcaag aattaaagaa cttataccaa
     6421 aacatatatt attagatgat ataatagctt caataagtta tgagttcaat atattctata
     6481 atataggaaa tattgatgat atagatcacc taggaaacag aagaataaga tctgtaggtg
     6541 aattgttaca aaatcaagtt agaattggtc tttcaagaat ggaaagagtt ataaaagaaa
     6601 gaatgacggt tcaagatatg gaagctataa cgcctcaagc gttagttaac ataagaccag
     6661 tttcagctgc aataaaagaa ttttttggta gttctcagtt atctcagttc atggatcaaa
     6721 caaatcctct atctgagtta acgcataaga gaagattatc agcccttggg cctggaggtc
     6781 tttcaagaga aagagctgga ttcgaagtgc gtgacgttca ccattcacac tatggtagaa
     6841 tgtgtccaat agagactcca gagggaccaa atatcggtct tataaactct ctaggaactt
     6901 atgcaaaaat aaatgaattt ggatttatag aatcaccata tagaaaattt gataaagaaa
     6961 catcaacagt tactgatgaa atacattatt taactgctga cgaagaagat ttattcgtaa
     7021 gagctcaggc aaatgagcca ttaacagaag atggtaaatt cgtaaaccat agagttgttt
     7081 gtagaactgt aaatggtgct gttgaaatgg ttcctgaaag tagagtagat tacatggata
     7141 tatctcctaa gcaggttgta tctgttgcca ctgctatgat acctttctta gaaaatgatg
     7201 atgccaaccg tgcccttatg ggagcgaaca tgcagcgtca ggcagtgcct ctagttagaa
     7261 gagaagcacc aattatagga actggtatag aatacagagc agcaaaagac tctggagcag
     7321 ttgttgttgc tagaaattct ggtatagcag aaagagtaac agcagatgaa ataattataa
     7381 aaagagaaga tggaaacaga gatagatata atctacttaa gtttaaacgt tcaaactcag
     7441 gtacttgtat aaatcaaaca cctataataa ataagggtga ccagataata aaaggtgatg
     7501 ttatagcaga tggtccagca actgatttag gagaagttgc acttggaaga aactgtctta
     7561 tagcattcat gacttgggaa ggttataact acgaggatgc tatattaata aatgaaagat
     7621 tagttaaaga agatagatta tcaacaattc atatagaaga atatgaatgt gaagctagag
     7681 atacaaaact agggccagag gaaataacta gagatatacc taatgttgga gatagtgcaa
     7741 ttaagaactt agatgacaga ggtataataa gaataggtgc agaagtagac tcaggtgata
     7801 tattagttgg taaagtaact ccaaaaggag aaactgaact tactgctgag gaaagattac
     7861 ttcgtgcaat atttggagaa aaggctagag aagttagaga tacttcactt aaagtgcctc
     7921 atggtgaatc tggtataata gttgatgtaa aagtatttac tagagaaaat ggagatgatt
     7981 tatctccagg agtaaatgaa ttagtaagat gctatatagc taaaaagaga aaaataaaag
     8041 ttggagataa aatggctggt cgtcatggta ataaaggggt tatctcaaga gtattacctg
     8101 aagaagatat gccattcatg gaaaatggaa caccactaga cataatactt aacccacaag
     8161 gtataccatc acgtatgaac attggtcagg ttttagaagt tcatttagga cttgctgcta
     8221 agacattagg atggtatgta gcaacatcag tattcgatgg tgctaatgag tatgacataa
     8281 tggatgctct tgaagaagct gggtatccta gagatggtaa attaacttta tatgatggta
     8341 gaacaggtga gtcttttgac aacagaataa ctgttggata tatgtactac ttgaaactac
     8401 atcacttagt agatgaaaaa ctacatgcaa gaagtactgg accatactca ttagttactc
     8461 aacagccact aggtggtaaa gcacaatttg gtggacaaag atttggagag atggaggttt
     8521 gggctctgga agcatatgga gctgctcaca tacttcaaga aatacttact gttaagtctg
     8581 atgacgttgt aggtcgtgtt agaacatatg aagctatagt taaaggtgaa aatatacctg
     8641 aaccaggaat cccagaatca tttaaggttc ttataaaaga acttcaaagt ttatgcttag
     8701 atgtaaaagt attgacagat gaagaccaag aaatagaagt aagagaatct gtggatgaag
     8761 atgatacaat aggtgaattt gagctagatg ttgtaaatca tatgggagaa gttgaagaaa
     8821 gtaatataat agaagaaatt gaagatgatt ttgcagaaaa tgcagaagat gaggatatag
     8881 agaatttaga agaattcact gaagatgatt tatttgaaga agaaatagat tttgatagtg
     8941 atgactttga tatgtaaatt taattaatgt aaatctcaaa tagaagggag agaactcctt
     9001 gtttgaatta aacaatttcg agtcgataaa aatagcattg gcttctccag aaaaaataag
     9061 acaatggtct aggggagaag ttaaaaagcc agaaactata aattaccgta ctttaaaacc
     9121 agaaaaagat ggtcttttct gtgaaagaat atttggacca caaaaagact gggagtgcca
     9181 ctgtggtaaa tatagaagag ttagatataa aggtgtagtt tgtgatagat gtggagtaga
     9241 agtaactaaa tcaaaagtaa gaagagagag aatgggacat atagagctag ctgctcctat
     9301 gtctcacatc tggtacttca aaggtatacc aagtagaatg ggacttttac ttgatatgtc
     9361 accaagatcg ttagaaaaaa tattatactt tgcctcatat gtggtagttg atccaggaga
     9421 gactggatta aacgaaaaac aattgcttac agaaaaagaa tacagaactg ctcttgaaaa
     9481 gtatggatat acttttactg taggaatggg tgctgaagct gtaaaaacat tactacaaaa
     9541 tatagattta gagcaacaaa gtaaagacct aagagcggag ttaaaagata gtacagggca
     9601 aaagaaagtt agaacaataa gaagattaga agttgtagag gcatttaaaa agtctggaaa
     9661 taaaccagaa tggatgattt tagatgcaat accagtaata ccaccagatt taagaccaat
     9721 ggtacaactt gatggtggaa gatttgcgac ttcagaccta aatgatttat atagaagagt
     9781 tataaataga aacaacagac ttaaaagatt attagagctt ggagctccag atataattgt
     9841 aagaaatgaa aaaagaatgc ttcaagaagc tgttgatgca ttgatagata atggtagaag
     9901 aggtagacct gtaacaggac ctggaaatag accacttaaa tctttatcag atatgttaaa
     9961 aggtaagcaa ggtcgtttcc gtcaaaactt acttggtaag cgtgttgact actcaggacg
    10021 ttctgttata gttgttggac cagaacttaa attttatcaa tgtggacttc caaagaagat
    10081 ggcattagaa ttattcaagc catttgttat ggataaatta gttaaagaag gttatgcaca
    10141 taatataaaa agtgcgaaat ctatagtaga aaaagttaag ccagaagttt gggatgtttt
    10201 agaagatgtt ataaaaagtc atccagttct tcttaaccgt gcaccgactc tacatagatt
    10261 aggtatacaa gcatttgaac caatcttagt tgaaggtaag gctataaaac tacatcctct
    10321 tgtatgtaca gcttataatg cagactttga tggtgaccaa atggcagttc atgtaccttt
    10381 atcagtagaa gcacaagcag aagcaagatt cttaatgctt tctgtaaata atatacttgc
    10441 tcctaaagat ggttcaccta taactactcc atctcaggat atggttttag gttgctatta
    10501 tctaacaata gaagcccaag atggagctaa aggaacaggt atggtattta aagactttaa
    10561 tgaactatta cttgcttatt acaataaatc agttcatcta catgcattag tgaaacttaa
    10621 ggtaacactt gaagatggaa gaagttcatt ggttgaaagt actgttggta gatttatatt
    10681 taatgaaaat atacctcaag acttaggttt tgtagataga aaagaaaatc catttgcact
    10741 tgaagttgat ttcttagcag ataaaaaatc tcttggtaaa ataatagata aatgctttag
    10801 aaaacacgga aatacagaaa cagctgaatt actagattat ataaaagctc taggatttaa
    10861 atattctaca ttaggtggca taacagttgc tgttgatgat atgagtgtcc cagaagaaaa
    10921 gaaagtattt atagctgagg cagaagctaa agttgataag tatgaaaaag catacagaag
    10981 aggtctaatc tctgatgaag aaagatatga aaaagttata gagacatgga cagaaacaac
    11041 tgataaagtt actgatgctc ttatgggtgg actagataga ttaaataata tatatataat
    11101 ggcacattca ggagccagag gttctaaaaa ccaaattaga cagctggcag gtatgcgtgg
    11161 tcttatggca aatgcatctg gtaaaacagt tgagatacca gttaaatcta atttccgtga
    11221 aggtttatca gtactagaat actttacatc ttcacatggt gccagaaaag gtcttgccga
    11281 tacagctata cgtacagctg aatctggata tttaacaaga agacttgttg atgtaagtca
    11341 agatgttatt gtaagggaaa tagattgtgg aacagaagat actacagaga tttatgctat
    11401 aaaagaagga aatgaagtta tagaagagat atatgataga attgtaggaa gatatactat
    11461 agaccctata ttaaatcctg aaactggaga agttatagtt gaggctgatt caatgataca
    11521 agaagatgaa gcagaaacta tagtagcttt aggaattgaa aaaattagga taagaacagt
    11581 tcttaactgt aaaactaatc atggagtttg ttctaagtgt tatggtagaa acttggcaac
    11641 aggaaaagaa gttaatatag gtgaagcagt tggtataata gcagctcaat ctatcggtga
    11701 gccgggtact cagcttacaa tgcgtacatt ccatactgga ggagttgcag gagctgatat
    11761 aactcaaggt ctaccaaggg ttgaagaatt atttgaagct agaaaaccaa aaggattagc
    11821 tgtaataact gaagtatctg gtagagttga aattgatgaa acaggaaaga gaaaagaagt
    11881 aaatgtaata ccagaagaag gcgagactca aacatatgta attccatatg gttctagatt
    11941 aaaagttaag caaggtcaaa tgctagaggc tggagaccct ctaacacaag gatttataaa
    12001 tcctcatgat atagtaagag taaatggtgt taagggagtt caagaatata tagttaaaga
    12061 agttcaaaga gtgtatagac ttcaaggggt tgatgttaat gataagcata tagaagttat
    12121 agtaagacaa atgctatcta aagtaaaagt tgaagaccca ggagatacag atttattgcc
    12181 aggtggatat gaagatgtat taacattcaa tgaatgtaat aaagatgcta tagataaagg
    12241 tctaagacca gcagttgcta aaagagtttt acttggtata actaaagcat ctcttgcaac
    12301 tgattcattc ttatcagcag cttctttcca agaaacaaca agagtattaa cagaggcagc
    12361 aataaaaggt aaagaagacc acttaatagg acttaaagaa aatgttatat taggtaagtt
    12421 aataccagca ggaacaggaa tgaagaaata tagaaatata gctgttgaaa aaattgaaga
    12481 ttaatagcat atagagtaaa gaaatcttga caaccattat ttgtggtgtt aaaatagtaa
    12541 agtgtgttgt ttaaaagtac actaagggct tgaacaagtt cttagtgact tttagatggc
    12601 aataaatgag aactaaaaag catcaagcta gaaaagtgct aatatcacat atttatgtgt
    12661 tatagacatt aaattgtaag taacgaatgt tacaatcagt cagtatgaat attcatattg
    12721 atgtagttgg cggtcactga taagatcgaa aatttggaag gaggtgcaga tgatgccaac
    12781 aattaaccaa ttagttcgta aaagtagaaa agcgctagag aagaaatcta ctgctccagc
    12841 attacaaaaa ggttacaact ctttaaataa gaaagtaact gacgctagtg ccccacaaaa
    12901 aagaggggtt tgtacttcag taaaaacagt tactcctaag aagcctaact cagctttaag
    12961 aaaagttgcc agagttagat taactaatgg tatagaagtt tctgcttata taccaggaga
    13021 aggacacaac ttacaagagc atagtgttgt tcttataaga ggaggaagag taaaagacct
    13081 tccaggggtt agataccata tactaagagg tactctagat acagcaggtg ttgataagag
    13141 aagacaatca agatctaaat acggtgcgaa gagacctaaa gaagctaaaa agtaaaaaca
    13201 attaatgatt gattatagtt cggtgcttta aatatatata tatttacggc atcacggcat
    13261 taaatgattt atgtcgagta cctatgattt aagttaaata attaaggagg gaagcgaaat
    13321 gccaagaaaa ggtaatattc caaaaagaga agttttacca gatcctatgt atgggagtaa
    13381 ggtagttact aaattaataa ataatctaat ggtagacgga aaaaaaggaa agtctcaaag
    13441 aatagtttat gatgctttcg caattgttgc tgaaaagaca ggagaagaag ctttagaagt
    13501 atttaataaa gcaatggata atataatgcc tgttttagaa gtaaaagcaa gaagagttgg
    13561 tggggcaaac taccaagtgc caatcgaagt tagacctgaa agaagacaaa cattaggttt
    13621 aagatggcta gtaaaatata ctagagctcg tggagaaaaa ggaatggttg aaaagctagc
    13681 taaagagata atggacgcag ctaacaacac tggagcttca gttaagaaaa aagaagatac
    13741 tcataaaatg gcagaagcta ataaagcatt tgctcattac agatggtagg atatatagat
    13801 atcttataaa attgaggata tcataaggag gataaccatg gctagaaagt ttcctttgga
    13861 aagaactagg aatataggaa tcatggctca tatagatgca ggaaaaacta ctactacaga
    13921 aagaatcctg ttttatactg ggcaaactca taaaatagga gaaactcatg aaggagcttc
    13981 tcaaatggac tggatggagc aagaaaagga aagaggtata acaataactt ctgccgctac
    14041 tactgcttca tggaaagacc atagaataaa cataatagat actcctggac acgtggactt
    14101 cacagttgag gttgaaagat ctctaagagt tcttgacggt tcagttgctg tattctgtgc
    14161 aaaaggtggg gttgagcctc aatcagaaaa tgtatggaga caagctgaaa cttatggagt
    14221 acctagaata gcttttgtaa ataaaatgga tatattaggt gcagatttct acaatgttgt
    14281 aagcatgatg aaatctagat taaattctaa tgcagttcct atgcaattac caataggtaa
    14341 agaagattca tttataggta taatagactt attaaaaatg gatgctgtta tatacaaaga
    14401 cgacctaggg gttgaaatgg aagaaacaga tataccagaa gatatgaaag aattagctgc
    14461 tgagtggaga gaaaaattag ttgaatcagt agctgaaact gatgaagaat taatgatgaa
    14521 ataccttgaa ggtgaagaac ttactataga tgaattaaaa gttgctataa gaaaagctac
    14581 tatagcttgt gaaatgaacc cagttttctg tggtacagct tatagaaaca aaggtgttca
    14641 gttagtaata gatgcagttt tagattactt accagcgcca actgatatac cagctataaa
    14701 aggtatatta gcagatggag aagaagctga aagacattct tctgatgaag agccattctc
    14761 tgcattagca ttcaaaataa tgactgaccc attcgttgga aaactagcgt tcttcagagt
    14821 ttattctgga acattagaat caggttctta tgttttaaat gcaactaaga ataagagaga
    14881 aagaattggt cgtatattac aaatgcatgc taatacaaga gaagaaataa ctaaagtgta
    14941 tgctggagat atagcagcag cagtaggatt aaaagatact actactggag acacattatg
    15001 tgaccctgct aatccaataa tacttgaatc tatggaattc cctgagccag ttatatctgt
    15061 tgctatagaa ccaagttcta aagcagctca agaaaaaatg ggtatagctc ttcaaaaatt
    15121 agctgaagaa gacccaactt tcacagttaa gactgaccaa gaaacaggac aaactataat
    15181 atctggtatg ggtgaattac acttagagat aatagtagat agattattaa gagaatttaa
    15241 agtagaagct aaagttggtg caccacaagt tgcatataga gagactataa ctcaaccagt
    15301 tgatgttgaa tacaagtact ctaagcaatc aggtggtaga ggacaatacg gtcacgttaa
    15361 gattagagtt gctcctcaag agccaggtga aggatacaaa ttcacaaaca aaactgttgg
    15421 tggatcagta ccaaaagaat atgttggtcc agttgacatg ggtatacaag gagctatgca
    15481 atctggtata gttgctggat atccagttgt agacgttgct gttgagttat atgatggttc
    15541 ttaccatgag gttgactctt ctgagatggc gtttaagatg gctggttcaa tggcattcaa
    15601 agatgctatg aagaaaggta atgcagttct tttagagcct tacttcaaag tagaagttgt
    15661 tactccagaa gattacatgg gagatgttat gggtgactta aactcaagaa gaggattaat
    15721 ccaaggtatg gaagctagaa gtggtgcaca agttataaat gcattcgttc cactttcaga
    15781 aatgtttgga tactcaacag acctaagatc ttcaactcaa ggtcgtgcaa cttacacaat
    15841 gatattcgat cactatgagc aagttccagc ttcagttgct aagaaaatag ctgaaggaaa
    15901 ataatttaaa aataattaat aatataaata taaaaaataa tcagctagat gagagatatc
    15961 tctcatctag cacaagat
//
LOCUS       DAYAPA010000096        15913 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.096, whole genome shotgun sequence.
ACCESSION   DAYAPA010000096 DAYAPA010000000
VERSION     DAYAPA010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15913
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.096"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            45..641
                     /locus_tag="ACGI59_002782"
     CDS             45..641
                     /locus_tag="ACGI59_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3793 family protein"
                     /protein_id="HFG9740561.1"
                     /translation="MSCCNSQFCCKNKIESSYIKWLLEVLGPVFLGSKPAEIINISFT
                     DVNREEKINDIYKYLYKCKKIDFIVIDKEKKGLKILFVNKKALSEKLKCKKTVNFLKF
                     LGYKQNTNVNAYLEHLVDKLKSDVFPDEIGIFLGYPLKDVIGFMGYSNYEVSMIKYWK
                     VYGDTKQSEDTYSKFLLHRKKMRKLLDYISVDKIVSCF"
     gene            complement(739..1698)
                     /locus_tag="ACGI59_002783"
     CDS             complement(739..1698)
                     /locus_tag="ACGI59_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrtA/SsuA/CpmA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFG9740562.1"
                     /translation="MTSLIMVLVGTVGCSTTNDKKDEPAKKEASLPKEINLTYVKSPL
                     NVPSILEKKDDLFGKEFKKDNIKVNFHELTTGPEQTQALAAGELDFLHALGGTSAIIA
                     ASNGVGLKITNIYSRSPKGYMLITNNDNIKSIKDLKGKKIVGPKGTILHQLLIAALAK
                     EGYTIDDVEYVNMDIPSAASALESKSADVAMLAGPVALKTINSGAKMIVNGEGLVSGI
                     IVTAVSDDFAEKYPELVKRFMKVHEETLKYMNENKDEVMDVVSKEVGLSLDETKEMYS
                     WYDFSSKITDKDIKELEDTQEFLMSNGMQQKKINIKDMLYNQN"
     gene            complement(1732..2463)
                     /locus_tag="ACGI59_002784"
     CDS             complement(1732..2463)
                     /locus_tag="ACGI59_002784"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9740563.1"
                     /translation="MVRSGFLLENIYKKYLVDNKEHLVLDNISLNISSEEITVILGES
                     GCGKTTLLRILAGLENATSGNVYFFNNDKKCTPKVGMVFQESRLMPWLNVSENILLHT
                     EKDNRNKVDLDKYLKMMKLEKFKNSYPNELSGGMAHRVSIARALSFNPDILLMDEPFA
                     ALDYFTRRKMQKEVVNIHKNTKKGVVFVTHNIEEAMEIAKKIIVFSKNKRIKQFSVED
                     EYNRDLTKNYYINLKKEILRELGEF"
     gene            complement(2456..3226)
                     /locus_tag="ACGI59_002785"
     CDS             complement(2456..3226)
                     /locus_tag="ACGI59_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9740564.1"
                     /translation="MFEKIHKRLKGYYIFLIILILWKIVCVLGIWSSYILPPPEIVLD
                     TFIKMIYDGSIFLNVYASVRRILIGFLISSLMAIPLGVLFGANKKMYEYFKPLFEFMR
                     STPPLALVPMLILWFGIGEESKIIIIILASFFPIFLNTLKGIKNCDNKLIEVGKSFNL
                     SKKRIFYKIIIPNSMLDIVVGLSLGLGYSFRAIIGAELIAASSGLGYLISDGKDMSRT
                     DVVLVGIFVIGFLGILSDYLFSKIITKFSKGKQVDLNG"
     gene            complement(3311..4021)
                     /locus_tag="ACGI59_002786"
     CDS             complement(3311..4021)
                     /locus_tag="ACGI59_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HFG9740565.1"
                     /translation="MFKFDVTNELMCIARGSGKFFAKKGAMVAFKGNFNFEKLLLGPS
                     NGGGLGRALLGHVQRSLTGEQMPIMVVEGEGEIYLAQNAYHVDVISIDPGDSIYVESE
                     NLLAFSEQLNYSVKLIGSGVISQKGLFTTHLVNKTGQNQDVAIITDGNPLILEGPCCV
                     DPDALVAWTGREPYPKVSKLSWKTFIGQTSGESYHMEFVEPGQIVIIQPSERLSGLKT
                     DSTPSDRMSSGGLRLGID"
     gene            complement(4201..4353)
                     /locus_tag="ACGI59_002787"
     CDS             complement(4201..4353)
                     /locus_tag="ACGI59_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HFG9740566.1"
                     /translation="MATFIIAAIVVVLMALAVMYMVKNSKKNGSSCGCGCSGCSSSKS
                     CHSAKR"
     gene            complement(4379..6535)
                     /gene="feoB"
                     /locus_tag="ACGI59_002788"
     CDS             complement(4379..6535)
                     /gene="feoB"
                     /locus_tag="ACGI59_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HFG9740567.1"
                     /translation="MSIKIGLIGNPNCGKTTMFNGLTGSSQYVGNWPGVTVEKKGGKL
                     KGNKDVEIVDLPGIYSLSPYTLEEVVTRNFMLDDKPDAVINIVDASNIERNLYLTTQV
                     LELGIPTVIALNMMDIVNKNGDKINIKELSEVIGCPVVEVTAVKGQGIMEAAEKAVEL
                     ASSNNKLNFKLPFVDESKDAIEKIEKIIEEKTPYIDVETRWLAIKLFERDENVIQKLD
                     ISKTILNSIEEITRNCEDELDDDSESIITANRYEFISSIISSIIKKNRKGKETVSDKI
                     DKIVTNRILALPIFALIMWGVYYIAVSSLGTIATDWTNDVLFGEIIQGNVSNFLASLN
                     VAEWLQGLVVDGLIGGVGAVLGFVPQIMLLFLLLSILEDCGYMSRVAFIMDRIFRKFG
                     LSGKSFIPMLISSGCGVPGVMSTRTIENDRDRKMTIMLTTFIPCGAKIPIIALFAGAL
                     FGGASWVAPSMYFLGIAMIIICGIILKKTSLFAGEPSPFVMELPQYHIPSAKGVLIHM
                     WDRGKAFIIKAGTIIFVACGVIWFLQSFNWSLQMVDAGDSILASLGNIVAPIFAPLGF
                     GNWQSSVATVTGLVAKENVVGTFGVLFGISDATEQDPTLLASVASMFTVASAFAFMAF
                     NMLCAPCFAAIGAIKREMGSWKWTWITLGFQTLTAYIIALLINQVGSLVLGTGGSIAG
                     AIISIFIAVAVVFVVLTYSNKNMKKEKMGKLSYMKN"
     gene            complement(6635..6856)
                     /locus_tag="ACGI59_002789"
     CDS             complement(6635..6856)
                     /locus_tag="ACGI59_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HFG9740568.1"
                     /translation="MNRLKDIKCGETVKVKKLEGEGATRRRIMDMGITRGVNIFIRKV
                     APLGDPIEVTVRDYELSIRKSDAEKIIVE"
     gene            complement(6869..7081)
                     /locus_tag="ACGI59_002790"
     CDS             complement(6869..7081)
                     /locus_tag="ACGI59_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HFG9740569.1"
                     /translation="MMPLILSNTGEEVVIKKISGSDKTRTFLNSIGFVVGTNVKIVSK
                     IDGNLIIDVKDTRVALDKKMASRIII"
     gene            complement(7402..10299)
                     /locus_tag="ACGI59_002791"
     CDS             complement(7402..10299)
                     /locus_tag="ACGI59_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFG9740570.1"
                     /translation="MKAFKKHFLILSLIFLLIISCFNNILCFADESENSKKIRVGYCD
                     DYGIISSSKEHSYTGYGYDYLREISKYTRWEYEFVKGSWEECLDRLEKGEIDLLGPLQ
                     KNDERNKLFNFPKLSSGYEYSALYTKDSNNNMFYEDISSFKGMRVAVLRGNFHNTAFE
                     KYREENNFSVEYIYCNSIDELIESVNEKKADAFVCGSIINAKGMKIVSKFSVEPFYFA
                     TAKDKPNLVKELDYALKELKINDMYYELELYKKYFKREVNNSIAFTRQEMNFIKANPK
                     LTMVYDSEWSPVEYYDEESNSFKGISSDLMSIISKKCGIKFEYIKTKNYNESLDYIKS
                     GKATMLCGSINENDKVKRFNMKLTNPYINIPMIMVGKLDTNLNNDLNIALTSSYKSID
                     SYIEKSFENAKTTSYKSVESCLNAINEGKANLMILSSYQFDELLREGKSENLSVISVL
                     DTSYGMRIGVSNKTDPILVSILNKSIDKITEEELSDCIYSNTIDKPYKVPFGVIFKEY
                     SIQIISFVCILFLIAIKYIIYNKKKKEDYLRKIAYTDPLTGADSIDKFKINSNKLFAK
                     NNPEEYALFYIDVDKFKYINDMFGYDMGNDTLIHISNTIASELKEDEIFARVSADHFV
                     LLIKYKTDDDIKTRLNNIYNKVQILSNPKINYYKLILDCGIYKISKSDNDINTIMDRA
                     NTARKTIKGGHKNSFAFYDKEMHKKILKEKEIENSMVDALNNGEFIVYFQPKYSLSDY
                     QIIGAEALVRWDNPQKGLIPPIEFIPVFERNGFIVNIDFYVFEEVCKKIREWMDEGQK
                     VVPISVNLSRMHFVNSNFIEEFKLIVDKYKIPTRLIELELTETAVLDNIEGLLDTMNN
                     LKEKGFVISMDDFGTGYSSLNLLKELPVDILKLDRAFFTEKDESNNEKIVISNVIKMA
                     KELKMKVISEGVETISQVEFLKQIGCDMVQGYLFSKPMPVKEFEKIAFKKE"
     gene            complement(10626..11234)
                     /locus_tag="ACGI59_002792"
     CDS             complement(10626..11234)
                     /locus_tag="ACGI59_002792"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HFG9740571.1"
                     /translation="MVDNLIKYIHKSDVAIVVLHEIYGINSFIEDVCQKYYKYGYDVF
                     CPELLGEKRVFPYSKVQEAYDFFINNVGFDIYKKVEKLINDLKSEYKYVFVAGYSVGA
                     TIAWRCSEDSFCDGIICCYGSRIRDYMDVIPKCPTLLVFAKHDSFDVDSISYQLSRKQ
                     NIQIEILDANHGFIDRYSNNYSDIQTKVFTIKEKSFLSKYLA"
     gene            complement(11494..12039)
                     /locus_tag="ACGI59_002793"
     CDS             complement(11494..12039)
                     /locus_tag="ACGI59_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH peroxidase"
                     /protein_id="HFG9740572.1"
                     /translation="MKKFVCTVCGYIHEGDAAPAQCPVCKVGADKFEEMKGEKNWADE
                     HRIGVAQGVDEEIIEGLRANFVGECTEVGMYLAMSRQADREGYPEVGEAYKRIALEEA
                     EHAAKFAELLGEVVVADTKENLRVRVDAEYGATDGKLKLAKKAKELGLDAIHDTVHEM
                     CKDEARHGKAFLGLLNRYFGK"
     gene            complement(12157..13980)
                     /locus_tag="ACGI59_002794"
     CDS             complement(12157..13980)
                     /locus_tag="ACGI59_002794"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9740573.1"
                     /translation="MNLSTGNQKVSRLKKKEKPKDFKKTFKRIFMYFKKDKKITRFIF
                     LLVIIDSIISTVIPYTIGKVVDIIDIDTYSKSIFLKGIIILLSFYIIEFSVKLSKGIC
                     TARLSQSIVKEMRSNLFLKFKNLPITFFDKSSTGDIMSRVTNDVDNISTGISNSLVQL
                     ITGILNILMTFIMMFVLSPILTFCSVILIPIVMFVSNFIAKRTRVFFKQQQVELGKLN
                     AHIEEMISNINVVKSFNYERKSVNDFKEVNDRILNISLKSQIYTSLLMPIMNVISNIG
                     FATICIIGGILASKDIITIGVIASFLSYVRQFTRPLNELANLFNTLLSAIAGCERVFE
                     IIDEQEEQEVHNMFKSEINDNKDGNLNSHFVKGEIELKDVSFSYDSKKKILDNINLKI
                     KAGTSNAIIGETGSGKTTIINLITNFYHIDEGSILLDGKDIYSINRNYLRTCFGVVPQ
                     EGYIFNDTVMENIRYGNLEATDEEVIYASKIANAHKFIVKMREGYSTVLSDRGEDLSE
                     GQKQLISIARAVLKNAPILILDEATSNIDIATEAKIQEAINNLTYGKTSIIIAHRLST
                     ISNCDNIIVLENGRIVESGNHKELIKLKGHYYRNILLTQGK"
     gene            complement(13993..15768)
                     /locus_tag="ACGI59_002795"
     CDS             complement(13993..15768)
                     /locus_tag="ACGI59_002795"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9740574.1"
                     /translation="MRYFKKYIKKYIVLFLTAVFFLTLEAFCDLAQPTIMAKIIDIGV
                     SNKNLDYILSTGAIMIGITFLGAIFAIIRSVISARVSQHFSYDLRQDVFEKINTYSLK
                     QIDKFGRASLITRITNDINQMQQFVNGMMRVFIKSPIMCIGSLIIAIKLNLKMSIIIL
                     IAVLIIFIIILLNMKIGYRLFKNVQESTDKVNSKLRQFLSGIKVVKLFCRYDYENQSF
                     EELNDELFKNSRKATTMMSFFRPTITIIINVSIIIILLIGRHLIYSGEIKIGIIVAYV
                     NYMTRILTSLIMISHVFNVFVRAKASYERISEVLVDENEEVIEREESLDVGEKDLKFV
                     NCDIIFENVDFSYNKNTDEKILKNVSFKIEEGQNIGVIGQTGSGKTSLVNLIQKLYII
                     DKGDIKIGGRSIYSISEESLRARIGVVFQNSSVFGKSILENVVMGNRDICKEDVVKSC
                     EIANANQFITKLENTYDEVLFQRGKNLSGGQRQRITIARAIAKKPQILILDDCFSAMD
                     INTETIIRKNIKEYISNLREKESKKCTVITISQKISSIIDCDKILVLDEGEVVGFATH
                     EDLLVSSPIYKKIFALQNLVSEDVN"
BASE COUNT         5279 a         2845 c         1565 g         6224 t
ORIGIN      
        1 ctaattactt gatattaatt ttcatttgaa ggaggttatc ttttatgtct tgttgtaatt
       61 cacaattttg ttgtaaaaat aaaattgaat catcatatat aaaatggctt ttagaagttt
      121 taggtccagt ctttttaggc tctaaacctg ctgaaataat aaatatttcg tttactgatg
      181 taaatagaga ggaaaaaatt aatgatattt ataaatatct atataagtgt aagaaaatag
      241 attttatagt tattgataaa gaaaaaaagg gattaaaaat cttatttgta aataaaaaag
      301 cattatcaga aaaattaaaa tgtaaaaaaa cagtaaactt tttaaagttt ttaggataca
      361 aacaaaatac gaatgttaat gcttatttag agcatttagt agataaactt aagagtgatg
      421 tttttcctga tgaaataggt atttttttag gatatcctct taaagatgtg attggattta
      481 tgggatatag taattatgaa gtttctatga ttaaatattg gaaagtttat ggtgacacaa
      541 aacaatccga ggacacatat tcgaagtttt tacttcaccg taagaaaatg agaaaattac
      601 tagactacat aagtgtagat aaaatagtaa gctgttttta attgtgtttt tgtataataa
      661 taaaaataca ccctctttta ccatacagtt tccataataa ataagggtgt attttccact
      721 ttaatatttt aaagtattct aattttgatt gtacaacata tctttaatat ttattttctt
      781 ttgttgcatt ccattagaca tcaaaaattc ctgtgtatct tctaattctt ttatatcctt
      841 gtccgttatt ttagaactaa agtcatacca agaatacatt tcttttgttt catctaaaga
      901 taaaccaact tctttagata caacatccat cacttcatcc ttgttttcat tcatatattt
      961 taaagtttct tcatgaacct tcataaatct ttttactaat tcaggatatt tttcagcaaa
     1021 gtcatcactt acagcagtaa ctattattcc acttactaat ccttctccat ttacaatcat
     1081 tttggcacct gaatttatcg ttttcaatgc cactggacct gcaagcattg ctacatcagc
     1141 acttttactt tctaatgcag aagcagcact aggaatatcc atatttacat attcaacatc
     1201 atctatagta tagccttctt tagcaagtgc agcaatcaac agttgatgaa gtatagtccc
     1261 ttttggacct acaatttttt taccttttaa gtcttttata gatttaatat tatcattatt
     1321 agttataagc atatatcctt ttggtgaacg gctataaata ttcgtaattt taagaccaac
     1381 accatttgat gcagctatta ttgcagacgt tcctcctaat gcatgtaaaa agtctagctc
     1441 acctgctgct aatgcttgag tttgctctgg tcctgttgtc aattcatgga aattgacttt
     1501 tatattgtcc tttttaaatt cctttccaaa taaatcatcc tttttttcta atatactagg
     1561 aacattaagt ggtgatttta cataagttaa atttatttcc ttaggtaaac tagcctcttt
     1621 ttttgcaggt tcatctttct tatcatttgt tgttgaacat cctacagtcc caaccaacac
     1681 cattattaaa gatgtcatta ctgctaaaat ctttttgttt cttttaatca attaaaactc
     1741 ccccaattcc cttaatattt cttttttcaa gtttatataa tagtttttag ttaaatctct
     1801 attatattca tcttcgacac taaattgttt aatcctttta tttttactaa aaacaattat
     1861 tttctttgct atttccatag cttcttcaat attgtgagtt acaaatacta cacctttttt
     1921 agtattttta tggatattta caacttcttt ttgcattttt cttcttgtga agtaatctag
     1981 tgctgcaaat ggttcgtcca tcagcaaaat atcgggattg aaggaaagtg ctcttgctat
     2041 acttactctg tgagccattc ctcctgataa ttcatttgga tatgagtttt taaacttttc
     2101 aagcttcatc atttttaaat acttgtctaa atcaacttta tttctattat ctttttcagt
     2161 atgtaaaagt atattttcac ttacattcag ccatggcata agtctacttt cttgaaatac
     2221 cattcctact tttggagtac actttttatc attattaaaa aaatatacgt ttccactagt
     2281 agcattttca agccctgcta aaattctaag tagcgttgtt ttcccacatc cactttcacc
     2341 taatataaca gttatctctt cactagatat atttaaagat atattatcaa gaactaaatg
     2401 ctctttatta tcaactaaat atttcttata tatattctct agcaaaaatc cacttctaac
     2461 catttaaatc aacctgcttc cctttgctaa attttgttat tatctttgaa aatagataat
     2521 ctgaaagtat acctaaaaat ccaataacaa aaattcctac caatacaaca tcagttctag
     2581 acatatcttt tccatcagaa atcaaatatc ctagtcctga agaagctgca ataagctctg
     2641 ctccaattat agccctaaaa ctatacccaa gccctaaact aagtccaaca acaatatcca
     2701 gcatagaatt agggattatt attttataaa aaattctctt tttagataaa ttaaaagatt
     2761 tacctacttc tattaattta ttgtcacaat tttttatacc tttcaatgta tttaaaaata
     2821 ttggaaaaaa ggatgccaaa ataataatta taattttaga ttcttcacct ataccaaacc
     2881 ataaaataag cattggcacc aaagctagtg gaggtgtact tcgcataaat tcaaatagtg
     2941 gtttgaaata ttcatacatt tttttatttg ctccaaataa aactccaagt ggtatagcca
     3001 tcaatgaact tattaaaaaa cctattaata tccttcttac actggcataa acatttaaga
     3061 aaatagaacc gtcatatatc atttttataa aagtatctaa gactatttca ggaggaggca
     3121 gaatatagct actccatata cccaaaacac atactatctt ccataaaatt aaaattatta
     3181 aaaaaatata atatccttta agccttttat gaattttctc aaacatataa aactcctttt
     3241 aacatatcta tccctgctac cctttttatt ttttaatatc ttaaataata attaagctat
     3301 atcataagat ttaatctatg ccaagtctta atccacctga actcattcta tcagaaggtg
     3361 tagaatctgt ttttaagcca gacaatcttt cagatggttg tataatgact atttgtccag
     3421 gttcaacaaa ttccatatga taagattctc ctgaagtctg accaataaaa gtcttccatg
     3481 atagttttga tactttagga tatggttctc ttccagtcca agctactaat gcatctgggt
     3541 caacacaaca aggtccttca agaattagag gattaccatc tgttattata gcaacatctt
     3601 gattttgacc tgttttattt actaaatgtg tagtaaaaag acctttttga gatattaccc
     3661 ctgaaccaat caattttaca ctgtaattta attgttcaga aaatgccaat aagttttcac
     3721 tctcaacata aatggaatca cctgggtcaa ttgatattac atctacatgg taagcatttt
     3781 gagctaggta aatttcacct tcaccttcaa ccaccattat tggcatttgt tctcctgtca
     3841 atgacctctg aacatgacct aataatgctc ttcccaatcc accgccatta cttggaccta
     3901 acagcaattt ttcaaaatta aaatttcctt taaaggctac catagcacct ttttttgcaa
     3961 aaaatttacc acttcccctt gctatacaca taagctcgtt tgtaacatca aatttaaaca
     4021 taatattcac ttatattgct taatttagca ataatccctc ctaatcatat atttatatat
     4081 aattatacag taaaattaca gcatagtcta acctacttca aatatgaaat ttgttattta
     4141 tacaaaaaat aagaacctga taataaagca atttaacttt ttatcaggct cttatcctat
     4201 ttatctctta gcgctatgac aactcttact acttgagcat ccagaacaac cacatccaca
     4261 actgctacca ttcttttttg aatttttaac catatacatt acagctagtg ccattaatac
     4321 tactacaata gctgctataa taaatgtagc catattcatc actccttatt tatatgaatt
     4381 aatttttcat ataacttaat ttacccattt tttctttttt catattttta tttgaataag
     4441 ttaaaactac aaagactact gctactgcta taaatataga tattattgcc ccagcaatac
     4501 ttcctccagt acctaaaact aaacttccaa cttgatttat aagaagagca attatatacg
     4561 ctgttaaagt ttggaatcct aatgtaatcc atgtccattt ccatgaaccc atttctcttt
     4621 ttatggctcc aatagctgca aaacaaggtg cacaaagcat attaaatgcc ataaatgcaa
     4681 acgcactagc tactgtaaac atacttgcaa ctgaagcaag aagtgttggg tcttgctctg
     4741 ttgcatctga tataccaaat aaaactccaa atgttccaac tacattttcc ttagctacta
     4801 gacctgtaac tgttgctact gatgattgcc aatttccaaa tccaagagga gcaaatattg
     4861 gtgcaactat atttcctaag ctagctaata tactatctcc agcatctacc atttgtaatg
     4921 accaattaaa tgattgtaag aaccagatta ctccacaagc aacaaatata attgttcctg
     4981 ctttgattat aaatgctttt cctctatccc acatatgtat taaaacacct tttgcacttg
     5041 gaatatgata ttgaggtaat tccataacaa atggagatgg ttctcctgca aataaactag
     5101 ttttctttaa tataattcca caaatgatta tcattgcaat acctaagaaa tacattgatg
     5161 gagctaccca tgaagcacct ccaaataatg cccctgcaaa taatgctatt attggaatct
     5221 tagctccaca tggaatgaat gtagttaaca taatagtcat ttttctatct ctatcattct
     5281 ctatagtacg agttgacata actcctggaa ctccacaacc tgaacttata agcattggta
     5341 taaatgattt tcctgaaaga ccaaacttac ggaaaattct atccattata aaagctacac
     5401 gtgacatgta cccacaatct tctaatattg ataataataa gaataaaagc atgatttgtg
     5461 gtacaaatcc tagtactgct ccaactccac caattagacc atcaactaca agtccttgta
     5521 accactctgc tacatttaaa gaagctagaa aattacttac attgccttga ataatttctc
     5581 caaacagtac atcatttgtc caatcagttg ctatagtacc caatgaactt actgctatat
     5641 agtaaactcc ccacattata agtgcaaaaa taggtaatgc tagtatacga tttgttacaa
     5701 tcttatctat cttatctgat acagtttcct taccttttcg attcttcttt attatacttg
     5761 aaattattga actaataaat tcataacgat tggctgttat tatactttca ctatcatcat
     5821 ccaattcatc ttcacaattt ctagttattt cttcaatact attcaatatt gttttagaaa
     5881 tatcaagttt ttgtataaca ttttcatctc tctcaaatag ctttatagct aaccagcgtg
     5941 tttctacatc tatatatgga gtcttttctt ctattatttt ttcaattttt tctattgcat
     6001 ccttactttc atcaacaaag ggtaatttga aatttaattt attgttacta cttgcaagtt
     6061 caactgcctt ttcagcagcc tccataattc cttgaccttt aactgctgtt acttctacca
     6121 ctgggcaacc tataacttca gatagttctt taatatttat cttatcacca ttcttattta
     6181 caatatccat catattaaga gctataacgg tcggtatacc tagctctaaa acttgagttg
     6241 taagatataa atttctctct atatttgatg catctacaat atttattact gcatccggct
     6301 tatcatctag cataaaatta cgtgtaacta cttcttctaa tgtatatgga gataaagaat
     6361 aaatacctgg taaatctact atttccacat ctttatttcc ttttaatttt ccaccttttt
     6421 tttcgactgt tactcctggc cagttaccaa catattgtga tgagcctgta agcccattga
     6481 acattgttgt cttaccacag ttaggatttc ctatgagacc tatttttatt gacatttcca
     6541 ttccctccat tattatttaa atacatcact gtttgtgata cagaaaatca ttctcattat
     6601 ataattacat taaccctact ctaattaatc tttattattc cacaataatt ttttctgcat
     6661 ctgacttacg aattgatagt tcataatctc tgactgttac ctcaattggg tcaccaagag
     6721 gagctacttt tcttataaat atattaacac ctcttgtaat ccccatatcc attatacgtc
     6781 ttcgtgtagc tccttctccc tctaactttt tgacttttac agtctctcca cacttaatat
     6841 ccttcagtct gttcatgtca ttacctccct atattattat tctacttgcc atttttttat
     6901 ctaatgcaac tctagtatct tttacgtcta ttattaagtt accatctatc ttagaaacta
     6961 ttttaacatt tgtacccact acaaaaccta tactatttaa aaaagtcctc gtcttatcac
     7021 ttcccgaaat tttctttata actacttctt cacctgtatt tgataatatc aatggcatca
     7081 tataatatcc ctcctcttaa agtaatttaa atctacaagc aatcgttatc ttatatataa
     7141 attacaatac cttaaatctt aatctattaa aataacatcc tattttttcc ttctttttac
     7201 aattcctaat tttgatttaa gtctatcaca atagtatatt ttttgcaagt gattattgta
     7261 ttcttttgat tttttttctc atttgattta ttttattaat aaaatcgtaa aaattaatac
     7321 aaaaataaag atgctctaaa atagtatact caactaatat taaagcacct ttattatata
     7381 tgtcattatt tacacattgc tttattcttt tttaaaagcg attttttcaa actcttttac
     7441 aggcattggc ttagaaaaca aatatccttg taccatatca cacccaattt gctttagaaa
     7501 ttctacttgc gaaatagtct ctacaccttc tgatattact ttcattttca gttcctttgc
     7561 cattttaatg acattagata taacaatttt ttcattatta ctctcatctt tttctgtaaa
     7621 aaatgctcta tctagtttta atatgtcaac aggcaattct tttaaaagat taagagagga
     7681 atatcctgta ccaaaatcgt ccatagagat tacaaatccc ttttctttca aattgttcat
     7741 tgtatcaagt aatccttcta tattatctaa tactgctgtt tctgttagct ctaattctat
     7801 aagtcttgtc ggaattttgt atttatctac aattaattta aactcctcta taaaattact
     7861 atttacaaaa tgcattcgag acaagtttac agatataggt actacttttt gtccttcatc
     7921 catccattct ctaatctttt tacacacttc ctcaaataca tagaaatcaa tatttacaat
     7981 aaaaccattc ctctcaaata caggaataaa ttctattggt ggtattagac ctttttgtgg
     8041 attatcccaa cgaacaagag cctctgcccc tataatttga taatctgaaa ggctatattt
     8101 aggttgaaaa taaacaataa attctccatt attaagagca tctaccattg aattttcaat
     8161 ttctttctct tttaatattt ttttgtgcat ttccttatcg taaaaagcaa atgaattttt
     8221 atgaccacct tttatagttt ttcttgcagt attagctcta tccataatag tatttatgtc
     8281 attatcactt ttacttattt tatatatacc acaatctaaa atcaatttat aatagtttat
     8341 tttaggatta ctaagtattt gaaccttatt gtaaatattg tttaaccttg ttttaatatc
     8401 atcatctgtc ttatacttaa taagaagaac aaaatggtca gcagaaactc ttgcaaaaat
     8461 ttcatcttcc tttaattcac ttgcaatagt atttgatata tgtatcaaag tatcatttcc
     8521 catatcatat ccaaacatat catttatata tttaaatttg tcaacatcta tataaaacaa
     8581 cgcatattct tctggattat tcttagcaaa cagtttatta gaatttattt taaatttatc
     8641 tatactatct gctcctgtca atgggtctgt atatgctatc ttccttagat aatcctcttt
     8701 cttcttttta ttataaatta tatatttaat agcaattaag aataaaatac atacaaaaga
     8761 aataatttgt attgaatatt ctttaaaaat cacaccaaat ggtactttat aaggtttgtc
     8821 aatagtatta gaatatatac aatcacttaa ttcttcttca gtaattttgt ctattgattt
     8881 atttaaaata gacactaata tagggtcagt tttatttgat acccctatac gcatcccata
     8941 gcttgtatct aaaacagata taacagataa attctcactc tttccttctc ttaaaagttc
     9001 atcaaactga taagaactaa gtatcattaa gtttgcttta ccttcattta tagcgtttag
     9061 acaactttct acactcttat aagatgttgt cttagcattt tcaaaactct tttctatata
     9121 agaatcaata gacttataac tactagttaa agcaatattt aaatcattat tgagattggt
     9181 atccaatttt cctaccataa tcattggaat atttatgtat ggatttgtca atttcatatt
     9241 aaatcgtttt accttatcat tttcattgat tgagccacat agcatagttg ccttaccact
     9301 ttttatgtaa tcaagactct cattataatt ttttgtttta atatattcaa attttatacc
     9361 acattttttt gatataattg acattaaatc ggaacttata cctttaaatg aattgctttc
     9421 ttcatcataa tactctacag gagaccactc tgaatcatat accatagtaa gttttgggtt
     9481 tgctttaata aaattcattt cttgccgagt aaatgctatg gaattattga cctctctttt
     9541 gaaatatttt ttatataatt ccaactcata atacatatca tttattttta attcttttaa
     9601 agcataatct aattctttta ctaaattagg tttatctttt gcagtagcaa aataaaaagg
     9661 ttctactgaa aatttgctaa ctattttcat tcctttagca tttataatac ttccacaaac
     9721 aaatgcatct gccttttttt cattgacgga ctcaattaat tcatcaatag aattacagta
     9781 tatatattca actgaaaaat tattttcttc tctatacttc tcaaatgcag tattatgaaa
     9841 gtttcctcta agtacagcta ctctcattcc cttaaaggaa cttatatctt cataaaacat
     9901 attattgtta ctatcctttg tatatagtgc actatattca tatcctgaac ttagtttagg
     9961 aaaattaaat agtttatttc tttcatcatt cttctgtaaa ggtccgagta agtctatttc
    10021 acccttttct agtctgtcta aacattcttc ccaagaccct tttacaaatt catattccca
    10081 tcttgtatat tttgagattt ctcttagata atcataacca tatcctgtat aagaatgctc
    10141 ttttgatgag gatattattc cgtagtcatc acaatatcca actcttattt tttttgaatt
    10201 ttcagactca tcagcaaagc atagtatatt gttaaaacat gatataataa gtaaaaatat
    10261 caaagaaagt atcaagaagt gctttttaaa tgctttcata tttaaaaacc tccaatttgc
    10321 caacttactt taacttattt cgttttctag ttgtaactac ttttattgta atggtaaata
    10381 tatcaaattg caatattctc atataatatt cttattcaac tataatgaat ttattattat
    10441 ctatttaaat acactatcta atattagttt cttataagtc ataaaagcac aactaacact
    10501 ataattctag tgattaattt tatttcaaaa aatataataa cactactaat attttaaata
    10561 tcaatagtgt cataaaaact tttcagatta attaaatctt tctatatatt ttataataat
    10621 ctaaatcatg ctaaatattt agacagaaaa cttttttctt taatagtaaa tacttttgtt
    10681 tgaatatcag aataattatt tgaatatcta tctataaaac catgatttgc atctaaaatc
    10741 tcaatttgga tattttgttt cctagaaagt tggtaagaaa tagaatcaac atcaaatgaa
    10801 tcgtgctttg caaatactaa taaggtagga cattttggaa ttacatccat gtaatctcta
    10861 atccgagagc cataacaaca aattatgcca tcacaaaaag aatcttcact gcatctccat
    10921 gcaatagttg ctcccacgct atacccagca acaaatacat atttatactc tgattttaaa
    10981 tcatttataa gcttttcaac ttttttataa atatcaaaac caacattatt tataaaaaaa
    11041 tcatatgcct cttggacttt tgaatatgga aaaactcttt tttctcccag aagttcagga
    11101 caaaatacat catatccata tttataatac ttttgacaaa catcttctat aaagctatta
    11161 atcccataga tttcatgcaa aacaacaata gctacatcac ttttatgtat atactttatc
    11221 agattatcca ccacctttac aaaacagcaa attttttata cttagtttaa tatattcttc
    11281 tttacatgtc aatttacttt tctaaaatca aatttatttt tggattaatc atttattttt
    11341 aaaagttgtg cattattact attaaattat tctctattat taaaaatata atcgttagaa
    11401 ataaaaaata aagtacaaat aattaaaaaa tatctcactc ataactttaa aaattataag
    11461 tgagatactc ctaaaatcta ctttatatta aatttatttt ccaaaatatc tatttaataa
    11521 tcctaaaaat gctttaccat gtctagcttc atctttacac atttcatgta ctgtatcatg
    11581 tatagcatct aatcctaatt cttttgcttt cttagctaat tttaattttc catcagttgc
    11641 accatactca gcatcaactc taactcttaa gttttctttt gtatctgcaa ctacaacttc
    11701 tccaagaagt tctgcaaatt tagcagcatg ttcagcttct tcaagagcta ttctcttata
    11761 agcttctcct acttctggat aaccttctct atcagcttgt ctactcattg ctaaatacat
    11821 tcctacttct gtacactcac caacaaagtt agctcttaat ccttcgatta tttcttcatc
    11881 tacaccttga gctactccta ttctgtgctc atcagcccaa tttttttcgc ctttcatttc
    11941 ttcaaattta tcagctccaa ctttacatac tggacattgt gctggtgcag catctccttc
    12001 atgtatatat ccacatactg tacaaacaaa tttcttcata attactttcc tcctaaattt
    12061 tatatatcaa attaaatttt ttatttatta agttagtcta tatatacccg caccatatac
    12121 ctttaaacaa tttttttaaa agttttttct aaatacttat tttccctgtg tcaataatat
    12181 gtttctataa taatgtccct ttaattttat caattcttta tgatttcctg attccacaat
    12241 tctaccattt tctaaaacta ttatattatc acaatttgat atggtactta atctatgagc
    12301 tataattata cttgtctttc cataagttaa attatttatt gcttcttgaa ttttagcttc
    12361 tgttgctata tctatgttac ttgtagcctc atccaaaata agtattggag cattttttaa
    12421 tactgctcta gcaatactta ttaattgttt ttgaccttca cttaaatctt cacccctatc
    12481 cgataataca gtactatatc cttcacgcat tttaactata aatttatgtg catttgctat
    12541 tttactagca tatataactt cttcatcagt agcctccaga tttccatacc ttatattttc
    12601 cataactgta tcattgaata tataaccttc ttgtggaact actccaaaac aagttctcaa
    12661 atagtttctg ttaatagagt atatatcctt accatctaaa agtatacttc cttcatctat
    12721 atgataaaaa tttgtaatta gatttataat agttgttttt ccacttcccg tctctcctat
    12781 gatggcattt gatgtgccag ctttgatttt taggtttata ttatctaaaa ttttcttttt
    12841 tgaatcataa gaaaaactta catctttaag ctcaatttct ccctttacaa aatgagagtt
    12901 taaattacca tctttattat cgttaatctc acttttaaac atgttatgca cttcttgctc
    12961 ttcttgttca tctataattt caaaaactct ttcacatcca gctatggctg aaagtaatgt
    13021 attaaataga tttgctaatt catttaaagg tcttgtaaat tgtcttacat aacttaaaaa
    13081 actagctata acccctatgg ttataatatc tttagatgca agaatgcctc ctataataca
    13141 tatagttgca aatcctatat tactaatcac attcataata ggcattaaaa gagaagtgta
    13201 aatttgcgat ttaagggaaa tatttagaat tctatcattg acttctttaa aatcatttac
    13261 agattttctt tcataattaa aagattttac tacatttatg tttgatatca tttcttcaat
    13321 atgtgcattt agttttccca actcaacttg ttgttgctta aaaaaaactc tagttctctt
    13381 tgcaataaaa ttactcacaa acattactat cggtattaat attacactgc aaaaggttaa
    13441 aattgggctt aatacaaaca tcataataaa agtcatcaat atgtttaaga ttcctgtaat
    13501 taactgtact aatgaatttg atattcctgt actgatatta tcaacatcat ttgtcactct
    13561 actcataata tctccagtag aacttttatc aaaaaaggtt attggtagat ttttaaattt
    13621 taaaaataaa ttacttctca tttctttaac tatactttga gataatctag ctgtacatat
    13681 ccccttagac agtttaactg aaaactcaat tatataaaat gaaagtaaaa taattattcc
    13741 ttttaagaaa atggattttg aataagtatc gatatctatt atgtcaacta cctttccaat
    13801 agtatatggt attacagttg atataattga atcaattatt actagcaaaa aaataaatct
    13861 agtaatcttc ttatcttttt taaagtacat aaaaattctt ttaaacgttt ttttaaaatc
    13921 tttaggcttt tcttttttct ttaatctact gaccttctga tttccagtac ttaaattcaa
    13981 tatacttcct ccttaattta catcttcact tactaaattc tgcaacgcaa aaattttttt
    14041 ataaattgga gaactaacca gtaaatcttc atgtgtagca aatccaacca cctcaccctc
    14101 atccaagact aaaatcttat cacagtcaat tatagaacta atcttttgtg aaatagttat
    14161 tacagtacat ttcttacttt ccttttctct taaattactt atatactctt ttatattctt
    14221 tcttataatt gtctctgtat taatatccat tgcactaaaa caatcatcaa gaattaatat
    14281 ttgtggtttt ttggctatag ctctagctat agtaattctt tgtctctgtc ctcctgataa
    14341 atttttaccc ctttggaata aaacttcatc ataagtattt tcgagttttg ttataaattg
    14401 atttgcattt gctatttcac atgattttac aacatcctct ttgcatatat ctctatttcc
    14461 cataactaca ttctccaata tactttttcc aaacacacta ctattttgaa atactacacc
    14521 tattcttgcc cttaaacttt cttcagaaat actgtatata ctacgtcctc caatcttaat
    14581 atcaccttta tcaattatat ataatttttg aattaaattt actaaagaag tctttcctga
    14641 accagtttga ccaatcacac caatattttg accttcttct attttaaagg atacgttttt
    14701 taatattttt tcatctgtat ttttgttata cgaaaaatct acattttcaa aaattatatc
    14761 acagtttaca aactttaaat ctttttctcc aacatctaaa ctttcttctc tctcaattac
    14821 ttcttcattc tcatcaacca aaacctcact tattctctca taagatgctt ttgccctaac
    14881 aaaaacatta aaaacatgtg aaatcattat aagagatgtt aatattctag tcatatagtt
    14941 tacataagca actataatcc caattttaat ttctcctgaa taaattaaat gccttccaat
    15001 tagtaatatt attattatgg aaacattaat gattattgta attgtcggtc taaaaaatga
    15061 catcatagtt gtagcttttc tactattttt aaaaagttca tcatttaact cttcaaagct
    15121 ttggttctca taatcgtatc tacaaaatag tttaactact tttattccac ttaaaaactg
    15181 tcttaatttt gaatttactt tatctgtact ttcttgtaca tttttaaata atctataccc
    15241 tatcttcata ttaagcaata ttataataaa tataattaaa actgcaatta gaataattat
    15301 tgacatcttt aaatttaatt ttatagctat aattaaactt cctatacaca ttattggtga
    15361 ctttataaaa actctcatca ttccattaac gaattgttgc atttgattga tatcattagt
    15421 aattcttgtt attaatgaag ctctaccaaa tttatctatt tgttttaatg agtatgtatt
    15481 tatcttttca aatacatcct gtcttaaatc ataagaaaaa tgctgagaaa ctcttgccga
    15541 gattacactt cttattattg caaatattgc acctaaaaaa gttataccta tcattatagc
    15601 tcctgttgat agtatgtagt ctaagttctt atttgaaacc cctatatcta ttatttttgc
    15661 cattatagtt ggttgagcta aatcacaaaa agcttccaat gttaaaaaaa atactgctgt
    15721 tagaaataat actatatatt tttttatgta ttttttaaaa tacctcattc aatcatccct
    15781 tcatagaatt ttctcattac caaaagtata tcataatggc taatttaatt gattatatca
    15841 attataaata tttgatttat tattaatttt tataccatat attttataaa ttatttgttt
    15901 ttatttccta tat
//
LOCUS       DAYAPA010000097        15893 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.097, whole genome shotgun sequence.
ACCESSION   DAYAPA010000097 DAYAPA010000000
VERSION     DAYAPA010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15893
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.097"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            123..1028
                     /locus_tag="ACGI59_002796"
     CDS             123..1028
                     /locus_tag="ACGI59_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFG9740575.1"
                     /translation="MKNSNFNILAFDGGGLKGALSISILERIVKEYPNLLNNINMFGG
                     TSTGSLIALGLAYGVSPKEIKELYSIENSKYIFNKSYAEILRPKYENKNLKEVLLSIF
                     PEELELKDLNKLVMIPSFYIGNEENAWKPVFYNNMPNSFTKTSKVVDVAMASSAAPVF
                     FPTYNRHVDGGIIATDPSLACIIHAIDSGFKLKNTRLFSIGTGYVYNSIKADTTEWGA
                     IDWIINKEPDLPIISITLEGNSQMSQLFSQKLLGDNYYRLNPKMEKDVAMDDCDALEY
                     LMSLGENCDIKDAFGWIKKKSFYRN"
     gene            1152..2105
                     /gene="msrA"
                     /locus_tag="ACGI59_002797"
     CDS             1152..2105
                     /gene="msrA"
                     /locus_tag="ACGI59_002797"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFG9740576.1"
                     /translation="MIKLATFSGGCFWCMVKPFDKYDGVIKVVSGYTGGHVENPTYQE
                     VCSGTTGHLEAIQITYDDDIISYDELLNIFWKQINPTDDGGQFGDRGTQYKTAIFYHD
                     EEQKNIALESREHLENSKIFDSKIVTDIIPATKFYEAEENHQDYYKKRPLNYNMYYKG
                     SGRYNFVKSHWDGNNINREELKKTLTPIQFEVTQNDMTEVPFENEYYDNNKEGIYVDI
                     VSGEVLFSSKDKFDSGCGWPSFSKPVIDKSIIERSDFSQGMYRVEVRSAKGNSHLGHV
                     FDDGPKELGGLRYCINSASLKFIPRENMEALGYGEYLKLFE"
     gene            2258..3388
                     /locus_tag="ACGI59_002798"
     CDS             2258..3388
                     /locus_tag="ACGI59_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFG9740577.1"
                     /translation="MNLKDFNGKRLKSARIFRAKTIEQLSKETKINKKDLKAFEENKY
                     VPNIENTLKLSNILNFPKEYFYKNENINIVVEDSHFNPQSKLPRVEEISYREKIIIIH
                     KIYSFMENYIKFPKENLPNRKVMSDININDIESLAYKTREFWDLNSTPIVNMVSLLES
                     KGIIISGMNVDRKGATIFTQKQRISKESKYLISLGNDKKSASIRNYTLACELGYIISS
                     ELRIPSKQFSEDEYACAFLLPKESFLKDLTHPEDLDYYVELKKKWIVPISAMIFRAYN
                     LEKINYKKYNYLMNEMDKKGWLIEEPLDKMKGSSPTYLKRAVELLIENKIMSVNSIVS
                     SLEEFGINLYPEDLELLMGLKKGLLSQEVNKKSKVIKFDGKK"
     gene            complement(3505..4473)
                     /locus_tag="ACGI59_002799"
     CDS             complement(3505..4473)
                     /locus_tag="ACGI59_002799"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFG9740578.1"
                     /translation="MAIKPRKISIVGSGHVGSHCGFSLITQGVCDELFMIDIDESKSK
                     AQALDLADAVSYLPHKVHIEKGTFSDCKDSDIVVISVADSSEGPLRRQNTTRLDLLRP
                     TIGMIKSIVKPIVDSGFDGIFVVISNPVDVVTNYIWEKSGFPKNKVIGTGTALDSTRL
                     RRILSEETGIAQQSIQAYSMGEHGDSQMVPWSHVSIGGKPILDMIKDNPSTYSNLDLP
                     SIVEKNKKTGISIINGKDCTEFGIGTALVEIVKAILHNEKKVLPVSTLLEGQYNERNV
                     FAGVPCVIGKDGIEEIIEINMTEYEQNEFNKSCSVLREYIALSKTL"
     gene            4808..4996
                     /locus_tag="ACGI59_002800"
     CDS             4808..4996
                     /locus_tag="ACGI59_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9740579.1"
                     /translation="MNYHTKEELVEALRVVSSSIINCGKGQKKFSEETSHHTRFKNII
                     EVMYISKSLIMDEISKRD"
     gene            5256..6455
                     /locus_tag="ACGI59_002801"
     CDS             5256..6455
                     /locus_tag="ACGI59_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HFG9740580.1"
                     /translation="MEKLYYTNQYIRDFTAEIIEIKEVENKYHVLLDKTAFFPGGGGQ
                     FGDLGKIENDDVINVYEDGKKIYHVLDKKPIKIHKVKCSIDWDRREDGMHQHFGQHVL
                     SGCFFKTFNANTTGFHLGNEYSTVDIEGNLEEDKIREIELFANEIIRQNIQVETLLPT
                     KKELKKIWLRRDLPNTDEEIRVIKIGDLDTNACCGVHPKSTGELRMIKIKKWEKHKSS
                     TRIEFLAGKRAVDEVLKRDIYLTKICRYLSCSEEDAINGIVNLNSKVEEALIKKRRLE
                     EVVSTYQVKEMIEKANKVGNISVIKKIYDDEDLKYVNKIANKIAEYENSIALLAVKFN
                     DKINLVFACSKNLKDINMGVLLKDAINLIDGKGGGSKVLAQGGGKNNGNLEATLDYAF
                     MKIEKTL"
     gene            6507..7010
                     /locus_tag="ACGI59_002802"
     CDS             6507..7010
                     /locus_tag="ACGI59_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HFG9740581.1"
                     /translation="MEQQKDYIELYENEELIEIREARMDDLDTIAKFNYNLAKETEGK
                     ELDMDVLTKGVKALLLDERKGKYHVYTVFDKVVAQIMYTYEWSDWRNGNFLWIQSVYV
                     DKEYRRKGIFNYLFNYIKNICDKDENIVGMRLYVEKENINAKATYESLNMYECDYNMY
                     EYEVVHS"
     gene            7117..7434
                     /locus_tag="ACGI59_002803"
     CDS             7117..7434
                     /locus_tag="ACGI59_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9740582.1"
                     /translation="MGVLKGMFYVFLFLDLVSIFCFFFNKGKIASNKIVFNAIGVLTF
                     VLCFMLFSYYPNNNLIGKFIASLFFIFGVAGVMLKEKNFLYARLLLTVVIVFSTLRLF
                     VIQ"
     gene            7592..8551
                     /locus_tag="ACGI59_002804"
     CDS             7592..8551
                     /locus_tag="ACGI59_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFG9740583.1"
                     /translation="MIESKKSEKLLLSIIYIIFLVYILMIIRIILFRDVPIYAIFKGT
                     FRNVNLIPFYTIYQFIVDSNLDFMKATVNIIENIGIFIPMGIFLPIVCKNLNKKTIII
                     VIILVSLAFELTQYIFALGSSDIDDVILNSLGGIIGINIYINMNKLFPNDINRFKAII
                     GTSLVLGVIGLGVISKNYHNLLTFKFRPDRKISKILIEENREIIKDINKDTVDIVGTF
                     ESFKKGIITIKAGSNNKVKRPENLIDSDGNIRIYLNENTKLVSYIITKESKMDIVKYE
                     EFDVKNLDLLRRYDTINVWIDKENQPKDKHGIMASKLLIGLYE"
     gene            8666..9451
                     /locus_tag="ACGI59_002805"
     CDS             8666..9451
                     /locus_tag="ACGI59_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9740584.1"
                     /translation="MEIIKYILYFFIYSFLGWTVESIGCSIASKRIINRGFLNGPICP
                     VYGFGAVIVISLLGRFNNVVIVFLLGMILTTILEYFTGFILETLFHAKWWDYSDRKFN
                     IKGRVCLKNAIYFGVMSVLIIRFIHPFIKYFVSIIPYRILISMAIITTLWTILDLIVT
                     IITLKKLDIKLNLLDDIITDLNDINVKLDKFDRGEIQALFKTINNDELEVREKINKIN
                     SKLDRIESNIILQKRVIKAFPHIKHKKQQEQLEHFKKIINEKR"
     gene            9743..11062
                     /locus_tag="ACGI59_002806"
     CDS             9743..11062
                     /locus_tag="ACGI59_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFG9740585.1"
                     /translation="MSEITSQMISTEEKKYVAKTQKIPYYPVAFKSGDGAMLYDYEGN
                     EYVDFLASAGSANVGHGNKEISQAVKEQMDDITQYTLAYFHSDPPVKLAEKLVEIAPG
                     DNDKKVLYSATGSACIDAAIKLARGYTGRTKIISMCESYHGSTYGAISISALSTNMRR
                     KMGPLLPEVYHFHYPDKNRTAKECLDEIEYAFAHYLPAEEVAAIFIEPIAGDAGIIVP
                     PVEWVQGLSKICKENGILLVSDEIQQGMGRTGKWFGIENFGVEADLIVLGKSVGGGLP
                     LGAVVGRTEIMQSLDAPAHLFTLAGNTTVCVAALKSIEIIEKENLLQKSIEMGDYIKA
                     GFEKLKEKYDIIGEIRGIGLSIGVDIVKGKGSNEKHPDATAKICYRCIQTGLIMIFLG
                     QSTLRVQPPLVITKEQVDKAMNIIDSAIDDYLNGRIGDEVYEVTQGW"
     gene            complement(11137..11565)
                     /locus_tag="ACGI59_002807"
     CDS             complement(11137..11565)
                     /locus_tag="ACGI59_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9740586.1"
                     /translation="MKDNNNTNKTIQFGEKKASLENNIQKNKNIKLDNTHKVIPYPKN
                     RKKDDKKKGNKKKNTNTSKVNKNNYGHSFFNNKYKYKRKPYVIALYTVILILLAILII
                     TKLTDSTNKGNYSRNTVSDTHKNDIRNIVFNESNNFLICG"
     gene            complement(11656..13032)
                     /gene="hydG"
                     /locus_tag="ACGI59_002808"
     CDS             complement(11656..13032)
                     /gene="hydG"
                     /locus_tag="ACGI59_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HFG9740587.1"
                     /translation="MFINHELINSLLEDAKNSTSDDIEKVLDKADRREKLSYKDIATL
                     LEVEDKKQLDRLFSIAGQIKNEIYGNRVVLFAPLYVSNYCVNECVYCGFSKCNKFKRK
                     KLTMEEIKEEVKILEKMGHKRLALEAGEDPKNCDINYILDCLDAIYSTYNENGNIRRV
                     NVNIAATSVDEYKLLKEKGIGTYILFQETYHKPTFIKMHGQSIKNDYYYHLTAFDRAM
                     EAGVDDVGAGVLFGLSDPKFEVLGLMMHNEHLEEKFGVGFHTISFPRLKKAEGMSLED
                     FPHLVSDDMFKKIVAITRLAVPFTGIIMSTRETAEMRNELLKYGVSQISAGSLTGVGG
                     YKAYEDGDNTEQFEVGDHRSPVEVLKELITDGYIPSYCTACYRKGRTGDRFMSLAKSR
                     QIHNVCTPNALTTLNEFLIDYGDEELKIMGKKLIAEEIGKIEREDIRNIVSNNMTALE
                     RGERDLYL"
     gene            complement(13033..14118)
                     /gene="hydE"
                     /locus_tag="ACGI59_002809"
     CDS             complement(13033..14118)
                     /gene="hydE"
                     /locus_tag="ACGI59_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HFG9740588.1"
                     /translation="MTKNKIKYFIDKLYSINSLSFDELLYLIENIDNEEYNFEQNLRN
                     YLYKQASDMRNKHYGNRVYLRGLIELTNYCKNNCYYCGIQSSNKNIIRYRLSLDEILE
                     CCDIGYKIGYRTFVLQGGEDAYFTDDKICEIVSSIKNKYEDCAITLSLGEKSYDSYKK
                     YFLCGADRYLLRHETATDSHYKKLHPPNIELRTRKECLKNLKEIGYQVGAGFMVESPF
                     QNNEDLVRDLLYLKELNPHMVGIGPFIPHHDTIFKDYKHGDLEKTLLMLSITRLLLPK
                     VLLPATTALASINPSGRNAGLLAGCNVIMPNLSPQEFRKQYSLYDNKAFTGQEACEYH
                     QSLEENIKSLGLEVDYSRGDNIEWRRL"
     gene            complement(14154..15362)
                     /gene="hydF"
                     /locus_tag="ACGI59_002810"
     CDS             complement(14154..15362)
                     /gene="hydF"
                     /locus_tag="ACGI59_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HFG9740589.1"
                     /translation="MSLNSTPQSVRVHIGLFGKRNAGKSSIINAITNQSAAIVSDIAG
                     TTTDPVFRPMEILPIGPCVLIDTAGLDDVGELGELRIGKSLDVLEKTDIALLVVDCQI
                     GISQEDLSLIEKFNDKNIPHILILNKIDTIKNQSEILNLTKNKVKCPVVSVSSTDKIG
                     IENLKNEIIKVLPKDSTEFKLVSDLIEPNDLVVLVVPIDKAAPKGRLILPQQQVIRDI
                     LDNGAISIVTKEDSLKETLSNLGKKPKLVITDSQVFPQVDKDTQKDIPLTSFSILFAR
                     QKGDLKELINGAYALENLKDGDKILMAEGCTHHRQTDDIGTVKIPNMIRKKTGKNITF
                     EFSSGVSFTEDINKYALVVHCGACMMNRAGMLSRIEKAKSFNVPIVNYGVLIAYVKGI
                     LERSLELFNY"
     gene            complement(15377..15628)
                     /locus_tag="ACGI59_002811"
     CDS             complement(15377..15628)
                     /locus_tag="ACGI59_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0065007 - biological regulation [Evidence
                     IEA];
                     GO_process: GO:0065009 - regulation of molecular function
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TM1266 family iron-only hydrogenase system
                     putative regulator"
                     /protein_id="HFG9740590.1"
                     /translation="MKKVAVISAILEEPKECQFKFNEVVSGFKGIIKGRMGIPCEEEG
                     VSIVCIAVVGEMDTINSLTGKLGNIPHVSVKTSISKKEV"
BASE COUNT         5994 a         1867 c         2500 g         5532 t
ORIGIN      
        1 gtaacttaaa tattaaaaga ggaacattgt aatatgtaac ttgcataaaa tatatatact
       61 atagtgaaat tattttgatt aaaatttaaa atataaggtg ttttatgtgg gggtatatgt
      121 atatgaaaaa ttctaatttt aatatattgg catttgatgg tggtggatta aaaggggcat
      181 tgagtatatc catacttgag aggatagtaa aagagtatcc aaatctatta aacaatataa
      241 atatgtttgg aggtacatct acagggagtc taattgcact agggcttgcg tatggtgtaa
      301 gccctaaaga gataaaagaa ctttactcaa ttgaaaattc aaagtatatt tttaataaaa
      361 gttatgcaga aatactcaga ccaaagtatg aaaacaaaaa cttaaaagaa gttttattaa
      421 gtatatttcc agaggaacta gaattaaaag atttaaataa attagtaatg ataccaagtt
      481 tttatatagg aaatgaggaa aatgcatgga aaccagtgtt ttataacaat atgccaaatt
      541 cttttacaaa gactagcaaa gttgtagatg tagcaatggc aagtagtgct gctccggtat
      601 tttttccaac atataatcgt catgttgatg gaggaataat agcaactgac ccgagtttag
      661 cgtgtataat tcatgccatt gacagtggat ttaaacttaa gaatacaaga cttttttcaa
      721 tagggactgg atatgtatat aacagtataa aggcagatac taccgaatgg ggagctattg
      781 actggataat taataaagaa cccgatttac caattatatc tattacctta gaaggaaatt
      841 ctcaaatgag tcagttattt tctcaaaaac ttcttggaga taattactat agattaaatc
      901 ctaagatgga aaaagatgta gctatggatg attgtgatgc attagaatat ctaatgtctt
      961 taggagaaaa ttgtgatata aaagatgctt ttggatggat aaagaaaaag agcttttata
     1021 gaaattagat tcaatttgca attgtatttt tacacatgat ttcattgagt aggtaaaaat
     1081 aataaaatgc agattattaa gttacttctt agggaataaa taaagtataa tttatttaag
     1141 gagtgatgaa aatgataaaa cttgctactt tttcaggtgg atgtttttgg tgtatggtaa
     1201 aaccttttga taagtatgat ggagttatta aagtagtttc tggatataca ggtggtcatg
     1261 ttgaaaaccc tacatatcaa gaggtatgct caggaactac tggtcattta gaagcaattc
     1321 aaataacata tgatgatgat ataataagtt atgatgaact tctaaatatc ttctggaaac
     1381 agattaatcc tacagatgat ggtggacaat ttggtgatag gggaactcaa tataagactg
     1441 ctatatttta tcatgatgaa gaacaaaaaa atatagcttt agagtcaaga gaacatctgg
     1501 aaaatagtaa aatatttgat agtaaaatag tgactgatat aattccagca actaaattct
     1561 acgaagcaga agaaaatcat caggattatt ataagaaaag accattaaac tacaatatgt
     1621 attataaagg gtctggaaga tataattttg taaaaagcca ttgggatgga aacaatataa
     1681 atagggaaga gttaaaaaag actctaactc ctatacaatt tgaagtcact caaaatgata
     1741 tgacagaagt accttttgaa aatgaatatt atgacaataa caaagaggga atttatgttg
     1801 atattgtaag tggagaagtg ttattttctt caaaagataa atttgattct ggatgtgggt
     1861 ggccaagttt ttcaaagcca gttatagata aatctatcat tgagagaagt gatttctcac
     1921 aaggtatgta cagagttgaa gtaagaagtg ctaaaggaaa ttctcattta ggtcatgtat
     1981 ttgatgatgg tccaaaagaa cttggaggtt taagatattg tataaattct gcttctctta
     2041 aatttatacc aagagaaaat atggaagctt taggatatgg agaatattta aaactttttg
     2101 agtaattagg aatatttatt ataaacttat ctaaaaggtg aaaatataaa aaaatatttt
     2161 caccttttgt ttttttgtca ataatatgat aaattattaa ttgtaatctt atttatacca
     2221 atagattaag aatttaattt tgaaagagag aaaacaaatg aatttaaaag actttaatgg
     2281 aaagagatta aaatcagcta gaatttttag agcaaagacg atagaacagt tgtctaaaga
     2341 aactaaaata aataaaaaag atttaaaagc ttttgaggaa aataagtatg taccaaatat
     2401 agaaaataca ttaaaattat ctaatatatt aaattttcca aaagagtatt tttataaaaa
     2461 tgaaaatata aatatcgttg tagaggatag ccactttaac cctcaatcta aactacctag
     2521 agtagaagag atttcttata gagaaaaaat aattataata cataaaatat attcatttat
     2581 ggagaattat attaagtttc caaaagaaaa cttgccaaat agaaaagtaa tgtcagatat
     2641 taatataaat gatatagaat ctcttgcata taagactaga gagttttggg atttaaatag
     2701 tactccaata gtaaatatgg taagtttgtt agagtctaaa gggattataa tatcaggtat
     2761 gaatgtagat agaaaagggg caactatctt tactcaaaaa cagagaatta gtaaagaatc
     2821 taaatatcta atatctctgg gtaatgacaa aaagtcagca tcaattagaa attatacttt
     2881 ggcatgtgag cttggatata taatatcaag tgaattaaga ataccaagta agcaattttc
     2941 agaagatgag tatgcctgtg catttttatt gccaaaagag agttttttaa aagatttgac
     3001 tcatccagaa gatttagatt attatgtaga actaaagaaa aaatggattg tacctatatc
     3061 tgctatgatt tttagagcat ataatctaga aaagataaat tacaaaaaat acaattatct
     3121 tatgaatgaa atggataaaa aaggctggtt gatagaagaa ccactagaca aaatgaaagg
     3181 ttcaagccca acgtatttaa aaagagcagt tgaattattg atagaaaata aaataatgag
     3241 tgtaaatagt atagtatcaa gtttagagga atttggaata aatctttatc cagaagactt
     3301 agaattattg atgggattga aaaaaggatt gttatctcaa gaggtaaata aaaaaagtaa
     3361 agttataaaa tttgatggta aaaaataggt ttatatttta gtattaaaat taaaagcagg
     3421 tacaaatgat ttaaacatta acttagttaa aggtcttttc aataagtcat ttacctgctt
     3481 ttagtgtaag aaagaatatt gaatctaaag tgtttttgaa agtgcaatat attcccttaa
     3541 aacagaacaa gatttattaa attcattttg ctcatattca gtcatattga tttcaataat
     3601 ttcttcaata ccatcttttc ctataacaca tggaacacct gcaaatacat ttctttcgtt
     3661 atattgacct tctagtaatg tagaaactgg aagaactttt ttctcattgt gtagtatagc
     3721 ttttacaatt tctactaaag cagtacctat accaaattct gtacaatctt taccatttat
     3781 tatacttatc cctgtttttt tatttttttc aactattgat ggtaagtcta aattgctata
     3841 agtacttggg ttgtctttta tcatgtctaa gattggttta ccaccaattg aaacatgaga
     3901 ccaaggaacc atttgagaat ctccatgttc acccatagaa taagcttgaa ttgattgttg
     3961 agcgattcca gtttcttcag ataaaatcct tctaagtctt gttgaatcta aagctgttcc
     4021 agttccaatg actttgtttt taggaaagcc agatttttcc caaatataat ttgtaactac
     4081 atcaacagga ttagatatga ctacaaatat accatcaaag ccagaatcaa ctatgggttt
     4141 tactatagat tttatcattc caatagttgg tcttagtaaa tctaatctag ttgtgttttg
     4201 tctacgtaaa ggcccttctg aagagtctgc aacacttatc actactatgt cagaatcttt
     4261 acagtcagaa aaagttcctt tttctatatg tactttatgt ggcagatatg aaactgcatc
     4321 agctaaatct aaagcttgag cttttgattt agattcatct atatcaatca taaagagttc
     4381 atcacaaact ccttgagtta ttaaactaaa tccacaatga gaaccaacat gccctgaacc
     4441 tacaatagat atttttcttg gctttattgc cattttaaat cactccccct aaaataacga
     4501 tacattgaaa attatagatg ttattaaaag ctgtttttgg atattttaat attctattat
     4561 ttcagaaaat ttatttaata tgattttata ctaaaatttt taaaaaagca atgttaatat
     4621 actctctatt agtgaatatt aaagtattat atgtatatta ataattatta aaatatgtaa
     4681 tgtcactgat agttagaata tttaagtgct aaaagatgtg tataaataat aatgatgtca
     4741 gtttataatg atattaaatt gagatatact gtttctctaa attaaaaaat aaaggagtaa
     4801 atgtgttatg aattatcata caaaagaaga attggtggaa gcactgagag tagtatcttc
     4861 aagtattatt aattgtggaa aaggacagaa gaaattttct gaggagacat ctcatcatac
     4921 acgttttaag aatattatag aagtaatgta tatttcaaaa tcattaataa tggatgaaat
     4981 tagtaaaaga gattgaagct tattttgcaa tttaacatgt tttaatatac attttattat
     5041 ataagaaaat aagtaaacat acatgttata aaatattgaa ataatataca attataaatt
     5101 tgttaacatt aatatctata tgttaaaata tagtagaaga tgttagtaaa taataggatg
     5161 agttctattt taatacaatt aaattaagct tagtttgaaa tttgtatcta aaaagtaaag
     5221 taatctagaa ttaagaaaat ggagatagaa ttataatgga aaaattatat tatacaaatc
     5281 agtatataag agattttaca gcagaaataa ttgaaataaa agaagtagaa aataagtatc
     5341 atgttttatt agataagaca gctttttttc ctggtggagg aggtcagttt ggagacctgg
     5401 gaaaaataga gaatgatgat gtaataaatg tgtatgaaga tggaaaaaaa atataccatg
     5461 tattagataa aaaacctata aagatacata aggtaaagtg tagtattgat tgggatagaa
     5521 gagaagatgg aatgcatcaa cattttggac aacatgtatt gtctggatgt ttttttaaaa
     5581 cttttaatgc aaatacgaca ggttttcatt tgggaaatga gtatagtaca gtagatatag
     5641 aaggtaattt agaagaggat aaaattagag agatagagtt gtttgccaat gaaattattc
     5701 gtcaaaatat acaagtagag acattacttc caacgaaaaa agaactaaaa aagatttggt
     5761 taagacgtga tttgccaaat actgatgaag agataagagt aataaaaata ggtgatttag
     5821 atacgaatgc atgttgtgga gtgcatccaa aatcaactgg tgaattgaga atgataaaaa
     5881 taaagaaatg ggaaaaacat aagtcaagta caagaataga gtttctagca ggtaaaaggg
     5941 cagtggatga agtattgaag agggatatat atttgactaa aatatgtaga tatttaagct
     6001 gtagtgagga agatgctata aatggtattg taaatctaaa tagtaaagtg gaagaggctt
     6061 taattaaaaa aagaaggctt gaagaagtag tatctacata tcaggtcaaa gaaatgattg
     6121 aaaaagcaaa taaggttggc aatatttcgg tcattaaaaa aatttatgat gatgaagatt
     6181 tgaaatatgt aaataagata gcaaataaaa ttgcagaata tgaaaatagt attgcattat
     6241 tagctgttaa atttaatgat aaaataaatt tagtttttgc atgttctaaa aatttaaaag
     6301 atattaatat gggagtttta ttaaaggatg caataaatct tatagatggc aagggtggtg
     6361 gaagtaaagt attagctcaa ggaggaggta agaataatgg aaatttagag gctacacttg
     6421 attatgcatt tatgaagata gaaaaaactc tataaatata ttatataaaa aaccttagta
     6481 gataaaatag atatggagga ataagtatgg agcaacaaaa ggactacata gagttatatg
     6541 aaaatgaaga actaatagag ataagagaag cacgtatgga tgatttggat acaatagcaa
     6601 aatttaatta taatttggct aaagaaactg aaggaaaaga acttgatatg gatgttttaa
     6661 ctaaaggtgt aaaagcattg ttattagatg agagaaaagg aaaatatcat gtatatacag
     6721 tatttgataa agttgttgca cagattatgt atacatatga gtggagtgat tggagaaatg
     6781 gaaatttcct ttggattcag agtgtatatg tagataagga atatagaaga aaaggtatct
     6841 ttaattattt atttaattat ataaaaaata tatgtgataa agatgaaaat atagttggaa
     6901 tgagattata tgtagaaaaa gaaaacataa atgcaaaagc aacatatgag tctttaaata
     6961 tgtatgagtg tgattataat atgtatgaat acgaagtagt acattcgtaa tataaatttg
     7021 taatactgtt atactgatgt tataaagtat tgattatatt gacgattata tgaaataaac
     7081 tggatattat aattagaatt tttagggggt ataaaaatgg gcgttttaaa aggaatgttt
     7141 tatgtatttt tatttttaga tttagtgtca atattctgtt tctttttcaa taaaggaaaa
     7201 atagcatcta ataaaattgt gtttaatgca ataggagtac ttacatttgt actttgtttt
     7261 atgcttttta gttattatcc taataataat cttattggaa aatttattgc ttctctattt
     7321 tttatatttg gagttgcagg agttatgttg aaagaaaaaa attttttata tgctagactt
     7381 cttttaactg tagttatagt tttttctaca ttaagattgt tcgttattca ataaaatgta
     7441 tttgtataaa aatttattaa agtctacaca tttaagtgta ggcttttttg attttttgtt
     7501 tgaaaattaa ggaaatttta aggaaatttt aaaatcgtct taaatattta ttgatatact
     7561 taaaatatta atagcgaaag gagacttatc agtgatagaa agtaaaaagt cagaaaaatt
     7621 gttactgtca attatataca taatattttt ggtatatatt ttaatgatta taagaataat
     7681 tttatttaga gatgtaccaa tttacgctat tttcaaggga acgtttagga atgtaaattt
     7741 aattccattt tatacaatat atcaatttat agtagatagt aatcttgact ttatgaaagc
     7801 tactgtaaat attatagaaa atataggtat atttattcct atgggaattt ttttacctat
     7861 agtatgtaaa aatctaaata aaaaaactat aattatagta attatacttg ttagcctagc
     7921 atttgaatta acacaatata tttttgctct tggaagtagt gatatagatg atgttatttt
     7981 aaattcatta ggtggcataa ttggaattaa tatttacata aacatgaata aattatttcc
     8041 caatgatatt aacagattta aggctataat aggtactagt ttagttttag gagttatagg
     8101 attaggagtt atttctaaaa attatcataa cttactaact tttaaattta gacctgatag
     8161 aaaaatatca aaaatattaa ttgaagaaaa tagggaaata attaaagata taaataaaga
     8221 tacagtagat atagttggga catttgagag ctttaaaaaa ggaataataa ctattaaagc
     8281 aggttctaat aacaaagtaa agagaccaga gaatttaatt gattctgatg gaaatataag
     8341 aatttattta aatgaaaata caaaattagt atcttatata attaccaaag aatcaaaaat
     8401 ggatatagtt aaatatgagg aatttgatgt gaagaattta gatttattaa ggagatatga
     8461 tactattaat gtttggattg ataaagaaaa tcagccaaag gataaacatg gtattatggc
     8521 tagtaaatta ttaattggat tgtatgaata aaatattaaa caagaataaa agaataacaa
     8581 atataaaaaa cgaaaaaact atttctataa ttatttttac gttgtgcata aaataagaac
     8641 attagtgaag gagtagagat aaattatgga gataataaaa tatatcttgt acttttttat
     8701 atattcattt ttaggatgga cagtagaaag tataggatgt tctatagcaa gcaaaagaat
     8761 aatcaacaga ggttttttaa atggtccaat atgtcctgtt tatggttttg gagcggtaat
     8821 tgtgatatca ctattgggaa gatttaataa tgtagtaata gtatttctat taggaatgat
     8881 tttaactacg atactagaat attttacagg atttatttta gaaaccttat ttcatgctaa
     8941 atggtgggat tattctgaca gaaaattcaa tataaagggt agagtatgtt taaaaaatgc
     9001 aatatacttt ggtgtaatgt ctgttttaat aataagattt atacatccat ttataaaata
     9061 ttttgtaagt ataattccat atagaatatt aatttcaatg gctataatta caacactttg
     9121 gactatactt gatttgattg ttactattat aacacttaaa aaattagata taaagttaaa
     9181 cttactagac gatataatta cagaccttaa tgatataaat gtgaaattgg ataaatttga
     9241 tagaggagaa atacaagctt tatttaaaac aataaataac gacgaattag aagttagaga
     9301 aaaaataaac aaaataaata gtaagttgga ccgtatagag tctaatatta ttttacaaaa
     9361 aagagttata aaagcttttc cacatataaa acataaaaaa caacaagagc aactagaaca
     9421 ttttaaaaaa attattaatg agaaaaggta attatacaat ttaatgaaga cgaatagaat
     9481 aatggtattt ataagatgat tttcagagga ctctataaaa atagaataga gtcctttatt
     9541 ttttgaaaaa taatgtttat ttgataaaaa taaaatatat tgtttctgaa taacaaatat
     9601 tttgaaaatt ataaaaacat atattgacaa tttgtgtcaa aaatactaac attaagtata
     9661 taaagaattt tatatgagaa tatctttata aatcgcaata attttaaaac ataaatttat
     9721 ttaaagaggg gatgatttgc caatgagcga gataactagt caaatgataa gtacagaaga
     9781 aaaaaaatat gttgctaaaa ctcaaaagat accttattat ccagtagcat ttaaatcagg
     9841 tgatggtgca atgctatatg attatgaagg taatgaatat gtagattttt tagcaagtgc
     9901 aggctctgca aatgtaggtc atggtaataa ggaaatctca caagcagtta aagaacaaat
     9961 ggatgatata acacaatata cacttgcata tttccatagt gatcctcctg taaaattagc
    10021 tgagaagctt gtggaaatag cccctggaga taatgataaa aaagtactat acagtgcaac
    10081 tggttctgct tgtatagatg ctgctataaa attagcaaga ggatatactg gaagaacaaa
    10141 gataatatct atgtgtgaat cttatcatgg aagtacttat ggagctatat ctatatctgc
    10201 tttaagtaca aatatgagaa gaaaaatggg tccattgtta ccagaagttt atcacttcca
    10261 ttatcctgat aaaaatagaa cagctaaaga atgtttagat gagatagaat atgcttttgc
    10321 acactatcta ccagcagaag aggtagctgc aatatttata gagccaattg caggagatgc
    10381 aggaataata gtgcctccag tagagtgggt acaaggttta agtaaaatat gtaaagaaaa
    10441 tggaatatta cttgtaagtg atgaaataca acaaggtatg ggaagaacag gtaagtggtt
    10501 tggtatagaa aattttggtg ttgaagcaga ccttatagtt cttggaaaat ctgttggagg
    10561 tggattacct ctaggagcag ttgttggaag aacagaaata atgcaaagtc ttgatgcacc
    10621 agcacattta tttactttag ctggaaacac aacagtatgt gtggctgctt taaaatctat
    10681 agagataatt gaaaaagaaa acttacttca aaagagtata gaaatgggag attatattaa
    10741 agcaggattt gaaaaactaa aagaaaaata cgatataatt ggagaaataa gaggcatagg
    10801 attatctata ggagttgata tagttaaagg aaaaggttct aatgaaaaac atccagatgc
    10861 tacagctaag atatgttata gatgtataca aactggatta ataatgatat tcttaggaca
    10921 atctacttta agagtgcaac cacctctagt aataactaag gagcaagtag ataaagcaat
    10981 gaacatcata gattctgcaa tagatgatta tttaaatggt agaattggtg atgaagtata
    11041 tgaagtaact caaggttggt aaaataaata ttaaatgaaa ataggagtta tctcaatttt
    11101 agaaaattat ttctgaaatg agataactct tttaagttac ccacaaataa gaaaattgtt
    11161 tgattcatta aagactatat ttcggatgtc gtttttgtga gtatcagaga cagtattacg
    11221 agaataatta cctttattcg tactatctgt taatttagtt ataattaaga tagctaaaag
    11281 aataagaatt actgtgtata aagcaataac atatggtttt cttttatatt tgtatttgtt
    11341 attgaaaaaa gaatggccat aattattttt atttacttta gatgtatttg tattcttttt
    11401 cttgttacct ttctttttat catccttttt tctattttta gggtaaggaa tgactttgtg
    11461 tgtattatca agtttgatat ttttattttt ttgaatattg ttttccaggc tagccttttt
    11521 ttctccaaat tgaatagttt tattggtatt gttgttatct ttcaatgtta atctccctcc
    11581 cttaaacttt ccctttagat aatattacta ctaacctatg gaatatacaa taaataatat
    11641 ataagtaata aaattttata ggtataaatc tctttctcca cgctctaagg cagtcatatt
    11701 gttagatact atgtttctta tatcttctct ttctatctta cctatttctt ctgcgataag
    11761 ttttttaccc ataattttaa gttcttcatc tccataatcg attaaaaatt catttaatgt
    11821 agtaagagca tttggtgtac atacattatg gatttgtcga cttttagcta agctcatgaa
    11881 tctatcgcca gttctacctt tacgataaca agctgtacaa taactaggta tatatccatc
    11941 agtaatcagt tctttaagca cctcaacagg gcttctatgg tctccaactt caaattgttc
    12001 tgtattgtca ccatcttcat atgctttata tcctccaaca cctgttagag aacctgcact
    12061 aatttgtgat acaccatatt ttagtaactc atttctcatt tcagcagttt ctctagtgga
    12121 cattatgatt ccagtaaaag gaacagccaa tctagttata gctactattt ttttgaacat
    12181 atcgtcagaa actaagtgag gaaaatcttc taagctcata ccttcagctt ttttcaatct
    12241 agggaaagat atagtatgga aacctacacc aaatttttcc tctaagtgtt cgttgtgcat
    12301 cataagtcct agaacttcaa atttagggtc agataaacca aataaaactc ctgcaccaac
    12361 atcatcaaca ccagcttcca tagctctatc aaaagctgtt aaatggtaat aatagtcatt
    12421 ttttatagat tgaccatgca tttttataaa tgttggtttg tgataagttt cttggaataa
    12481 tatataagtt cctattcctt tttcttttag taatttatac tcatctacac ttgtagctgc
    12541 tatgttaacg tttacccttc ttatatttcc gttttcatta tatgttgaat aaattgcatc
    12601 taagcaatct aagatgtaat ttatatcaca atttttaggg tcttcaccag cttctagagc
    12661 aagtctttta tgacccattt tttctaatat tttaacctct tcctttatct cttccatggt
    12721 aagttttttt cttttaaatt tgttgcattt actaaaaccg caataaacac attcatttac
    12781 acagtagttt gatacataaa gaggggcaaa taaaaccact ctattaccat atatttcatt
    12841 ttttatttgc ccagctatac taaataatct atctagttgt tttttatctt ctacttcaag
    12901 aagtgtagct atatctttat aactaagttt ttcccttcta tcagccttat caagtacctt
    12961 ttcaatatca tcagaagttg aattttttgc atcttctaat aaagaattaa taagttcatg
    13021 gtttatgaac atttacaaac gcctccattc aatattatca ccgcgagaat agtcaacttc
    13081 aagtcctaat gacttgatgt tttcttctaa actttggtga tattcacaag cttcttgacc
    13141 agtaaaggct ttgttatcgt aaagagaata ttgttttcta aattcttgag gagaaaggtt
    13201 tggcataatt acattgcaac cagctaaaag acctgcattt ctaccactag ggtttataga
    13261 tgccaaggct gttgtagcgg gtaataaaac ttttggaagt agaagtcttg taattgaaag
    13321 cataagtaat gtcttctcta aatctccatg tttataatcc ttaaaaattg tatcatggtg
    13381 tggaataaaa ggtcctattc caaccatatg aggatttaac tcttttaggt ataataaatc
    13441 acgaacaagg tcttcgttgt tctgaaatgg tgattcaacc ataaatccag cacctacctg
    13501 ataaccaatt tctttaaggt tttttaggca ttcttttcta gttctaagct caatgtttgg
    13561 cggatgtaat tttttataat gactgtctgt agcagtttca tgtcttaaaa gatatctatc
    13621 tgcaccacat aaaaagtatt ttttatacga atcataactc ttttctccta gtgatagagt
    13681 tatggcacaa tcctcatatt tattttttat acttgaaaca atttcacata ttttatcatc
    13741 agtaaaatat gcatcttcac caccttgcaa aacgaatgtt ctgtatccta ttttataacc
    13801 tatatcacaa cattcaagta tttcatcaag tgaaagcctg tatcttatga tatttttatt
    13861 agaagattgt attccacaat agtagcaatt atttttacaa tagtttgtaa gctctataag
    13921 tcctctcaag tagactctat ttccataatg tttatttctc atgtcgctag cttgtttata
    13981 aagatagttt cttaaatttt gttcgaagtt atattcctca ttgtcgatat tttctattaa
    14041 atatagtagt tcatcaaaac ttaatgaatt aatgctgtaa agtttatcta taaagtattt
    14101 aatcttattt ttagtcatat tcaaatatgt aattattaag tatagttaaa tttttaataa
    14161 ttgaaaagtt caagtgatct ttccaaaata ccttttacat atgctattaa gacaccatag
    14221 tttacaatag gtacattgaa agattttgcc ttttctattc tagataacat tccagctcta
    14281 ttcatcatac aagctccaca gtgaactaca agggcatatt tatttatatc ttcagtgaag
    14341 gatactccag aactaaattc aaaagtaatg tttttgccag ttttctttct aatcatatta
    14401 ggaattttta ctgttccaat atcatcagtt tgtctatgat gggtacatcc ttcggccatc
    14461 aagattttat caccatcttt taaattttct aaagcataag ctccattaat caattcttta
    14521 aggtctccct tttgtctagc aaacaatata gaaaaagatg taagaggaat atctttttgt
    14581 gtatctttat ctacttgtgg aaatacttga gagtcagtaa taacaagttt tggttttttg
    14641 cctaaattag atagagtttc ttttaaacta tcttcttttg taactataga aattgcacca
    14701 ttatccaaaa tatctcttat aacttgctgt tgaggaagta taagtctacc ttttggagct
    14761 gctttatcta ttggaacaac taaaacaact aaatcatttg gttcaattaa atctgatacc
    14821 aatttgaact cagtgctatc cttaggcaaa acttttatta tttcattttt taaattttca
    14881 attccaattt tatctgttga tgagacagaa acaactggac atttaacttt atttttagtt
    14941 aagtttaaaa tttctgattg attcttaata gtatcaattt tgttaagaat tagaatatga
    15001 gggatattct tatcattaaa tttttcaatt agtgacaaat cttcttgtga tataccaatt
    15061 tggcaatcca ctactagtaa agctatatct gtcttttcta aaacatctag tgatttacct
    15121 attctaagtt caccaagttc accaacatca tcaagaccag cagtatctat aagaacacaa
    15181 ggtcctatag gaaggatttc cattggtcta aatactgggt ctgtagtagt tccagcaata
    15241 tctgagacta ttgcagcact ttgatttgtt atagcattta ttatgctaga tttacctgca
    15301 tttcttttac caaagagacc tatatgtact cttaccgatt gtggagtaga atttagactc
    15361 atttgtactc accaccttaa acttcttttt ttgatatact agtttttaca gatacatgag
    15421 gtatgttgcc caatttacca gtcaaactat taatagtgtc catttcgcca acaactgcta
    15481 tacatacgat tgagacacct tcttcttcgc aaggaatgcc cattcttcct ttaataatac
    15541 ctttaaaacc tgatacaacc tcattgaatt taaattgaca ttcttttggt tcttccaaaa
    15601 tagcacttat aactgcaacc tttttcataa atatcctcct aactcagaaa atatgtctta
    15661 aacaaatcca aaatcaaaaa acttttcttt gtaaagaaaa gtttctacaa aagaaaagtt
    15721 attaattaca caagtataat gtgctatatt aaactatttc cttacagatt agataactct
    15781 tttctggtag ggttagcttg taaaaattta atttaacaac aagtaactga agaaaatttt
    15841 tatacaattt atataaatta taataaaaat taactaaaga aaattgaagt tac
//
LOCUS       DAYAPA010000098        15838 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.098, whole genome shotgun sequence.
ACCESSION   DAYAPA010000098 DAYAPA010000000
VERSION     DAYAPA010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15838
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.098"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            164..1354
                     /locus_tag="ACGI59_002812"
     CDS             164..1354
                     /locus_tag="ACGI59_002812"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFG9740591.1"
                     /translation="MISNKMQTLVANSSVIRAMFEEGKKLSDIYGEENVFDFSIGNPS
                     VEPPETIKAVINDILNEESPNLVHGYMNNSGYEDVRDAIAEHINKKDGLNLTRENLIM
                     TCGAAGGLNIILKTLLNPGDEVIAFAPYFGEYKNYTENYDGKLIEVPTNIETFEPDLD
                     ALKNAITPKTRALIINTPNNPTGVIYSEELLKNLGELLDSKQKEFNTSIYLISDEPYR
                     EIIYDGAKVPCVLKYYRNSFIGYSYSKSLSLPGERIGYIVANGQMDDFDDVMSSLNVA
                     NRILGFVNAPSLFQRVIARSLDAEVDVNIYKKNFDLLYNSLIDMGYSCVKPNGTFYLF
                     PKAPIEDDKKFCNDAKQFNLLLVPGSSFGCPGHFRVSYCVSYDKVKSSLPAFEKLAKL
                     YNLK"
     gene            1524..2486
                     /locus_tag="ACGI59_002813"
     CDS             1524..2486
                     /locus_tag="ACGI59_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFG9740592.1"
                     /translation="MKKGYVYIILTTLFFSSMEISLKTVTNDFNPLQITLSRFFVGGL
                     VLLPFAVKRLRALSLSITKSDLKYFAFLGFMCVVVSMSLYQLAVLNTKASVVAVLFSC
                     NPVFVMLLAYLILKEKIYRHNVISLILEVLGIIVIINPLHTKLTLSGIFLTLSSAIIF
                     SMYTVFGKRKTLKFGGIVVTCFSFIFGSLEMLILVLLTKINFIADILNKNKLHVFANI
                     PIFSGYTLHNMPIMIYVFVFVTGVGYALYFMAMEATSTSLTSLVFFFKPVISPILALL
                     ILKEIIPINMVIGILLIVIGSIISIIPTIITQMHNKHVEALYKE"
     gene            complement(2608..3270)
                     /gene="zmp1"
                     /locus_tag="ACGI59_002814"
     CDS             complement(2608..3270)
                     /gene="zmp1"
                     /locus_tag="ACGI59_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease Zmp1"
                     /protein_id="HFG9740593.1"
                     /translation="MRPSKKLLIAIISIFLISSVPVSAHADSTTIQQNKDTLSQIVVF
                     PTGNYDKNEANAMVNRLANIDGKYLNALKQNNLKIKLLSGKLTDEKEYAYLKGVVPKG
                     WEGTGKTWDDVPGLGGSTVALRIGFSNKGKGHDAINLELHETAHAIDHIVLNDISKSA
                     QFKQIFAKEGRSLGNVNYLGVYPEEFFAESFAYYYLNQDTNSKLKSACPQTYSFLQNL
                     AK"
     regulatory      complement(3370..3455)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(3641..6559)
                     /locus_tag="ACGI59_002815"
     CDS             complement(3641..6559)
                     /locus_tag="ACGI59_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021362860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SrtB-anchored collagen-binding adhesin"
                     /protein_id="HFG9740594.1"
                     /translation="MKKGNRKALLISLIMILSMVVSTIYPTVSYASELGENSQIQSGS
                     TNSSTGEEKESDNKKPEQTPEKDKATDNKKPEQTPEEEKPTDNKKPEQTPEEDKSTDN
                     KKPEQTPEGEKPIDNKKPEQIPEEDKSTDNKKSEQALEDEKPLDNKNTEKTPEEDNLL
                     ENENLLKVLEEELNEENEDYGFVVKINNNTIETESMKKISFNLTYTPTSKGIQAGDSI
                     TFKVPDVFNKVNLDYTSECFDKTESNGEYTLTFRELPNGQSVMQGKIGLEAYVKKVDE
                     DTNAKIHIETTGKIESGSGDIDVEIKPGDKTDVPDAKGTLKKLVEGKKSTTVFMPVKN
                     KDINYSIQVNEKQEELKDIILYDELPEGLTLINGSVSVVTSDGKEVSDFNIEQSKNSI
                     SVNLGNIDKSYTVKYKARISDKNAKHGNKYKNVARIESDGKKIQEDDATVSIFDRGDD
                     YLLTKGHSGATNITQVGQVINYQISINDDKSPISNVVITDNIPEGMRLTTSGEAGHDF
                     RVVEIPMNGSWTPWSKEKIANNISYKVEEKRNESGQVDKVITGFTINLSKEEVESKFF
                     IAYTLKVISIEDSYINRAVLDANNSEIDKNDEINFKKNSGLISAKKEVDKKVLNSSDN
                     QIVKYKINMSTYGVYDAGQVNLLDEVNSALEISNIKYSDNLELKKEAGDGKNTIRLVN
                     KYEFKQIKEGEPVQSWVTFDADFTNVKVGETIRNVAQINGSSPPGVETTKQGYAFEAK
                     KVDALDKNVLSGAKFNLEDAFGNIVVKDLVSDEDGIIQSSIKKPGTYYLVEIMAPSGY
                     EKLKDKIKVEIGNKDIGRIVDIGNIENIRQENPPVNPPAPPNTDEPIVNPPVPPSTDK
                     PRKPSSSSDTEDTIVINPPIPPSEDIINPPIPEVLNPPVPPSEEMIETPVKQIIPIPE
                     VVKPSVSEEKNNKVKDDTLVNPPVPPKTGDSTTIIGEILLVIGAIVGLIVLRRNKNTN
                     "
     regulatory      complement(6677..6762)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            complement(6889..8067)
                     /locus_tag="ACGI59_002816"
     CDS             complement(6889..8067)
                     /locus_tag="ACGI59_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFG9740595.1"
                     /translation="MFSKILVKTFIRDSENVQNTDVRNKYGYVAGVVGILSNLLLFVI
                     KVFIGMLTSSIAIMADAFNNLSDMASSAITMIGFKLASKPADKEHPFGHGRIEYLSAL
                     IVAFMVMLVGLQFVKSSIERIVNPIPVKFEVIPLILLIASIMIKIWLSRFNKFMGNKI
                     DSSALKAVSLDALGDVFTSSCVVISFIVARFTNFPIDGYVGIVVSLVILYAGFSLVKD
                     TINPLLGEAPDEEMVNSIIELLLSYKYIIGTHDLIIHNYGVGRCIASIHAEIPSNIDI
                     MEIHEIIDTAEREISEKLDIYLVIHMDPICLEDKEVMSAKKELEEILKKNSLVKSMHD
                     FRIVGKGSKKNLIFDIVVNPSEFSKNMSEDDLKEDITKLVKEINPEYNCVIVVDKDFI
                     "
     gene            8442..8627
                     /locus_tag="ACGI59_002817"
     CDS             8442..8627
                     /locus_tag="ACGI59_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFG9740596.1"
                     /translation="MFFISDIYTKSPIKFDTPLQKEAYKILQKLDIDFECVDTDEAIT
                     MEDCVQINKKLNMKMVI"
     gene            complement(8692..11466)
                     /locus_tag="ACGI59_002818"
     CDS             complement(8692..11466)
                     /locus_tag="ACGI59_002818"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005388 - P-type calcium transporter
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HFG9740597.1"
                     /translation="MRYYNKPTKEVLKYLKTNPEIGLDDNEVEERKLRYGLNEFTIKE
                     GRTFWDELGESLTEPMILILIGAAVISSFVGELHDALGILGAIFIGISIGIITEGKSK
                     KAAHALSKLTENIEVKVLRNGKIIKISKNDLVPGDIVYIETGDMIPADGRLIQSINLK
                     LREDMLTGESDDVAKNADAVLDMEVVYSKTEIIEQDAIPAKQVNMVFGGTLVAYGRGI
                     MVVTHTGDKTEMGKIAQNLSNEDQQTPLQIKLGKLGAKIAGISGIIATLLCMFMIIQM
                     QRKGMLILDTSSILSFLQSLEPAKNAYMVCIALIVATVPEGLPTMINITLAITMQKMA
                     KINALVTKKEACETIGSVSVICSDKTGTLTQNKMMVEVAYVDGKYISGGEYQSNSYFE
                     QNCIVNSTADIEKEDNSFKYIGSATECALLLYHNDKNYNEMRKQTYLISQIPFSSEEK
                     KMSTLIRQEDSDILLSKGAPEVLLKKCSYVQQGKNIVPITPKVEKSILDEIKKLQIKS
                     MRTLGFAYKKMSNSKTEVAMTSEGELNLIGNSRSYMKEDNLVFSGFVGIVDPLREGVK
                     DSIDKAFNAGVDVKMLTGDNINTATAIGNELGLLNDGKKAVEATYIDVLTDKELREEI
                     KGISIVARSKPDTKMRIVSALQKSGEVVAVTGDGINDAPALSQADVGIAMGISGTEVS
                     KNAADIILTDDSFSTIVEGIKWGRGIYENFQRFIQFQLTVNIVAFIIAIISQLTGKDM
                     PFTTIQLLWVNIIMDGPPALALGLEPVRDYVLKRKPINRHSGIIARSMFVNIIINAIL
                     IITIVFTQSAFNILGATSEEQGTVIFSLFAFSALFNALNCREFGLNSTIPNFFKNKLA
                     LQIIVVTGIIQIIFTQVFQSFFNSVSLDFDMWIKIILFASTILLSNEFVKLILRTMKN
                     SRTMNFNSKN"
     gene            complement(11638..12894)
                     /gene="glyA"
                     /locus_tag="ACGI59_002819"
     CDS             complement(11638..12894)
                     /gene="glyA"
                     /locus_tag="ACGI59_002819"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFG9740598.1"
                     /translation="MLKTTKISDPELYKIVADELVRQEHNIEMIASESTAPTEVLELS
                     GCVFTNKTEEGYPGARFQAGSEEADKLETLAIKRAKEVFGAEHVNVQPYSGSTANYCV
                     YSSILKPNDTVLSMRLDQGGHLTHGSAVNFLHDIYKYEFYGVDPNTGRIDYDALEAKA
                     KECRPKLIIAGASSYPRLIDYERISKVAKEVGAYFMVDMAHVAGLVAAKVIPSPVPYA
                     DFVSSSTTKTFCGPRSGIVLCKAEHAKKLDKGVFPGTLGSIHLNTVAAKAFSLLYLST
                     DKFKKIMEQVVVNAQTLASELISHGFSIVSGGTDNHIVMVDLRSKNLTGKQFEKALEY
                     VGITVNKNVIPDDPQSPFVTSGVRIGLTSISQRGLKEKEVIQIAGIMNKVAENIDNKE
                     VLDECKAEAQELISKFPLYPEGYFED"
     gene            complement(12966..14468)
                     /locus_tag="ACGI59_002820"
     CDS             complement(12966..14468)
                     /locus_tag="ACGI59_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HFG9740599.1"
                     /translation="MRALNVVERAGNKLPDPVTIFLLLCIIVVILSAVISNLGVEEIH
                     PSTKEVVKVVNLLEKEQIQSYLGSIVTNFQSFAPLGLVLVTMLGAGVAEKSGFMEVLM
                     KKGISKVPQKLVTVAIVFAGMLSHTAADVGFIILPPLAALVFLGIGRHPLVGMFAAFA
                     GVAGGFAANVMLSTTDVLLAGFTIPAAQMMDPSYQGNPAMNLYFAFISAIVLTFAGAF
                     VTEKFIAPRFTKYNDSTNDKDIQELSELENKGIKYALLSLLVVVVVIVALCIGDNAFM
                     KDPETGSILSSNAPLMKGIVPIITIIFLTPGLVYGKVSKKIKSDKDLVSMMGSSMSDM
                     GGYIVLAFIASQFINLFNLSNLGTILSITGAKLLAESGIPSYGLIIGFILLSGFINLF
                     VGSASAKWAILAPIFVPMFMLLDFNPALTQIAYRIGDASTNPISPLFPYFPVILAFAR
                     RYDKDIGIGTVISNMIPYSVVFTLIEIIILLLFMGIGIPLGPGGGISYVL"
     gene            complement(14559..15749)
                     /locus_tag="ACGI59_002821"
     CDS             complement(14559..15749)
                     /locus_tag="ACGI59_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFG9740600.1"
                     /translation="MHELILISEKYKEDILDFFTDIHMHPELSFKEFRTTKAIKDLLV
                     SLDIEILDLGMETGVVGLLKGKYDGPTVALRGDIDALPIYEEVDIKYKSRYDGIMHAC
                     GHDIHTSCLVGCAYVLSHIRDSLHGNVKFIFQPAEEVNKGAKMLVERGVMENPKVDAI
                     FGLHNHPDIPCGKIGVKLGGLMAAVDTIKIEVNGFGGHGGIPNRTIDPIVASSAIIMG
                     IQTIVSRNISPLESAVISIGTINGGTANNVISEKVDMTGTCRSFSNEVRKKISENLEN
                     IVCEIARGYQATAKLDYLFDLPAVINSKEMYAIACKSVCDLYSEDAIVDPIPSTGGED
                     FSIFMEKAPGFFYWLGVGNKEQDCIYQWHNPKFKADKNSILVGTNVLCQSVINYMDKL
                     KNKI"
BASE COUNT         4975 a         2737 c         1930 g         6196 t
ORIGIN      
        1 ataaaattag aaattggaat ttttaatatg tactgtacat aaatatattt tatataaaag
       61 tttatatttt caaacaagta atttttcgaa aattgtctaa attcagtaaa aatatggtaa
      121 tatatacata agcaatataa atctataaaa aggagtgtta catatgatat ctaataaaat
      181 gcaaacttta gttgcaaata gttctgttat aagagctatg tttgaagaag gaaagaaact
      241 ttctgatatt tatggtgagg aaaatgtttt tgattttagt ataggaaatc caagtgttga
      301 acctccagag actattaaag cagttataaa tgatatttta aatgaagaat ctccaaattt
      361 agttcatgga tatatgaaca actcaggata tgaggatgta agagatgcta ttgctgaaca
      421 cataaataaa aaagatggat taaatcttac aagagaaaat ttaattatga cttgtggtgc
      481 tgctggtggt ctaaatatta ttttaaaaac tcttttaaat cctggtgatg aagttattgc
      541 ttttgcaccg tattttggtg agtataaaaa ttatacagaa aactatgatg gaaaattaat
      601 agaagttcct acaaatatag agacttttga accagattta gatgctctaa aaaatgctat
      661 tactccaaaa acaagagctt taataataaa cactccaaat aatcctactg gtgtcatata
      721 ttctgaagaa ttattaaaaa atcttggaga attattggat tcaaaacaaa aagaatttaa
      781 tacaagcata tacctaattt ctgatgagcc atatagagaa ataatttatg atggtgccaa
      841 agttccttgt gtactaaaat actatagaaa ttcatttata ggatactctt atagtaaatc
      901 attatctcta cctggtgaac gtataggata tattgttgct aatgggcaaa tggatgattt
      961 tgatgatgtt atgtcttcat taaatgttgc aaatagaata cttggatttg taaatgctcc
     1021 ttctcttttt caaagggtta ttgcaagaag tttagatgca gaagttgatg taaatattta
     1081 taaaaagaat tttgaccttc tatataatag tttaatcgat atgggatatt cttgtgttaa
     1141 acctaatggt acattctatt tgttcccaaa agctccaatt gaagatgata aaaaattctg
     1201 caatgatgca aaacaattta atttattatt agtaccaggt tcttcttttg gttgtccagg
     1261 tcattttaga gtatcatatt gtgtatctta tgataaagtt aaaagttctt taccagcatt
     1321 tgaaaaatta gccaaattat ataatttaaa ataactaaat caaagtaatt caaataatta
     1381 actaccttag tatagaatac ttacattcta tattaaggta gtttataatt ttatgtacta
     1441 tattttatac ataccatatt attccaatat gcttgaatat tcttatctat agtactaata
     1501 taaattacaa ggaggtgttt gacttgaaaa aaggttatgt ttatataata ctgactacct
     1561 tattttttag ttcaatggaa attagtctaa aaactgtcac taatgacttt aatcccttac
     1621 aaataacttt atcaagattt tttgttggcg gtttagtctt actacctttt gctgtaaaaa
     1681 gattaagagc tcttagtctt tctattacaa aatcagactt aaaatacttt gcatttttag
     1741 gttttatgtg tgtagtagta agtatgtcat tgtatcaact tgctgttcta aacacaaagg
     1801 catctgtagt tgctgttttg ttcagttgta atccagtctt tgtaatgctt ttagcttact
     1861 taattttgaa ggaaaaaata tataggcaca atgtaatttc acttatatta gaagttcttg
     1921 gaattatagt tataataaat ccattgcata caaaactaac tcttagtgga atattcttaa
     1981 cattatcttc tgctattatt ttttcaatgt atactgtatt tggtaaaaga aaaaccttaa
     2041 aatttggtgg aatagttgta acctgcttta gctttatatt tggtagtttg gaaatgttga
     2101 tattggtttt actcactaaa attaatttta tcgcagatat attaaataaa aataagcttc
     2161 atgtatttgc aaatatccct atttttagtg gatatacctt acacaatatg cccattatga
     2221 tttatgtatt cgtatttgtt acaggtgttg gttatgcgct ttactttatg gcaatggaag
     2281 ctacatctac tagtcttaca tctttagtat tcttctttaa accagtaata tctccaatac
     2341 tagcattatt aatacttaaa gaaattatcc ctataaacat ggttatagga attttattga
     2401 tagtaattgg ttcaataatt tctattatac ctacgataat tactcaaatg cataataaac
     2461 acgttgaagc cttgtataag gagtagtaat tgataatcta tcatagttat acataagaca
     2521 aaatttatta gatttaaacg caatataaaa gagatgctat tataatagca tctctttatt
     2581 tatatttatt ttaaatttat taaaactcta tttagctaaa ttttgcaaaa agctataagt
     2641 ttgtggacaa gctgatttta acttactatt tgtatcttga tttaaatagt aatatgcaaa
     2701 tgattctgca aaaaattctt ctggatatac acctaagtag tttacattac ctaaagaacg
     2761 accttcttta gcaaatattt gtttgaattg tgctgattta gatatatcat ttaatactat
     2821 atggtctatt gcatgtgctg tttcatgtaa ttctaaattt attgcatcat gccctttacc
     2881 tttgttacta aatcctattc taagagctac tgtacttcca cctaaacctg gaacatcatc
     2941 ccaagttttt ccagtacctt cccatccctt aggaactaca ccttttaaat aagcatattc
     3001 tttttcatct gttaactttc cacttaataa ttttattttt aagttatttt gctttaatgc
     3061 atttaaatac tttccatcta tattagctaa tctatttacc atagcattag cttcgttttt
     3121 atcgtaattt ccagttggaa aaactactat ttgactaagt gtgtctttat tttgttgtat
     3181 agtagtacta tctgcatgtg cacttactgg aactgaactt attaagaata ttgatattat
     3241 agctattaat aattttttac ttggtctcat attattccct cctttgagtt aggccgggag
     3301 taataaattt caaaataaaa aagataccca agagtatctt tgtatataca aaaagaaatt
     3361 tttactcttt tcggcaactg gctggcatgg tttactaaac tttagcccct atagctttgc
     3421 gtgactaggt ttccctagtt ttgcctcatt taattttata tctatattat agcttatatc
     3481 tttttaattg tctatatccc tttggaactt ttttctaaaa agttattatt tttttacata
     3541 aatcttcaaa aaaaaatatc ttctaacaat attttcatta ttaaatattg ttttagaaga
     3601 tattttttaa tataacttat attttttata atgttagggt ctaatttgta tttttatttc
     3661 ttcttaatac gataagtcct acaatagctc ctataaccaa aagtatttct ccaataattg
     3721 tagtactatc ccctgttttt ggtggtactg gtggatttac caaagtatca tctttaactt
     3781 tattattttt ttcttctgac actgatggct ttactacttc tggtattgga ataatctgtt
     3841 taacaggagt ttctatcatc tcttctgatg gtggtactgg cggattcaat acctctggta
     3901 ttggcggatt tattatatct tctgatggtg gtattggtgg atttataact atagtatcct
     3961 ctgtatcaga tgaacttgat ggtttccttg gtttatctgt gcttggtggt actggtggat
     4021 ttactattgg ttcatctgta tttggtggtg ctggtggatt tactggtgga ttttcttgtc
     4081 ttatgttttc tatatttcca atatccacta ttctaccaat atctttatta ccaatttcaa
     4141 ccttaatttt atccttcagt ttttcatatc cacttggagc cattatctcc actaaataat
     4201 atgttcctgg tttttttata ctagattgaa ttataccatc ttcatcacta actaaatcct
     4261 taactactat atttccaaat gcatcttcta aattgaactt agcacctgaa agtacatttt
     4321 tatctaaagc atccactttt ttagcttcaa aagcataacc ttgcttagta gtctctactc
     4381 ctggaggact tgaaccatta atctgagcca catttcgtat agtctcccca acttttacat
     4441 ttgtgaagtc tgcatcaaat gtaacccaac tttgcacagg ttccccttct tttatctgtt
     4501 taaattcgta tttatttact aatcttattg tattttttcc atctccagct tcttttttaa
     4561 gttcaagatt atcagaatat tttatattag atatttctag agctgagttt acttcatcta
     4621 aaagatttac ttgtcctgca tcataaaccc cataagttga catattgatt ttgtatttaa
     4681 caatctggtt atcactacta tttaaaactt ttttatcaac ttcttttttt gcgcttatta
     4741 atccagaatt tttcttaaag tttatctcat catttttatc aatttcacta ttatttgcat
     4801 caagaacagc tctatttata tagctatctt caatactaat tacctttaat gtataagcta
     4861 taaaaaactt tgattccact tcttctttac tgagattaat tgtaaatcca gttataactt
     4921 tatctacttg cccactttca tttctttttt cttctacttt atatgaaata ttattggcaa
     4981 ttttttcttt actccaagga gtccagctcc cattcattgg aatttctaca actctaaaat
     5041 catgcccagc ttctcctgaa gtagttaatc tcatgccttc tggtatatta tctgttataa
     5101 caacattact tattggtgat ttatcatcat ttattgaaat ctgatagttt attacttgac
     5161 caacttgagt aatatttgtt gctcctgaat gtcctttagt aagaagataa tcatctcctc
     5221 tatcaaatat acttacagta gcatcatctt cttgtatctt cttaccatca gattcaatcc
     5281 tagctacatt cttatattta ttaccatgtt ttgcattttt gtcactgatt ctagccttat
     5341 acttaacagt atacgattta tctatatttc caagattaac tgaaatagaa tttttactct
     5401 gttcaatatt aaaatcacta acttcttttc catcacttgt tactacactt acagaaccat
     5461 ttatcaatgt caatccttca ggaagctcat catacaaaat aatatctttt aactcttcct
     5521 gcttttcatt tacttgtata ctgtaattta tatctttatt tttaactggc ataaatacag
     5581 tagttgattt ttttccttca acaagctttt taagtgttcc tttagcatct ggaacatccg
     5641 ttttatctcc aggttttatt tctacatcta tatctccact tccactttct atttttccag
     5701 tagtttcaat atgtattttt gcattagtat cttcatcaac ctttttaaca tatgcttcca
     5761 aacctatttt accttgcatt acactttggc cattaggtaa ttctctaaat gtaagtgtat
     5821 actctccatt actttctgtt ttatcaaagc attcacttgt ataatctaaa ttaactttat
     5881 tgaatacatc tggcacctta aaagttattg aatctcctgc ttgtattcct tttgatgttg
     5941 gagtataagt taaattaaat gatatttttt tcattgattc cgtttctatt gtattgttat
     6001 taatttttac aacaaatcca tagtcttcat tttcttcatt aagttcttct tctaaaactt
     6061 tcaataaatt ttcattttct aatagattat cttcctctgg agttttttct gtatttttat
     6121 tgtctagtgg tttttcatct tctaaagctt gttctgactt tttattatct gttgacttat
     6181 cttcttctgg tatctgctct ggctttttat tatctattgg cttttctcct tctggagttt
     6241 gctctggctt tttattatct gttgacttat cttcttctgg ggtctgttct ggttttttat
     6301 tatctgttgg tttttcttcc tctggggtct gttctggttt tttattatct gttgccttat
     6361 ctttctctgg agtctgttct ggctttttat tgtcgctttc cttctcctct ccagttgatg
     6421 aattagttga accactttga atctgactat tctctcctaa ttccgaagca taagatacag
     6481 ttggatatat tgtagacacc accatactta aaatcataat taaactaatt aataatgcct
     6541 ttctatttcc tttcttcatg taaatctcac ccctttaggt tacctataat ttataatcta
     6601 atcttccatt aatatcgtat ataaagtaat ttttactaaa aaattataca aaaaaagcta
     6661 tgaaaataaa taaatttata ttcatagccg gttgcaccac tgactcagta tatccataag
     6721 gtgcccaata atagaatata cttcaacatc tctataatat ttcataaaaa caattaaaat
     6781 tgctttattt atattaaaaa tacaattata tacttctatt atattatgcc tctatactat
     6841 tgtctatcct ttttacattt tttaccttat gatgagatta attaataatt aaataaaatc
     6901 cttatctaca acaattacac aattatattc aggatttatc tccttaacta attttgttat
     6961 atcttctttt aagtcatctt ctgacatgtt tttggaaaat tcggaaggat taactactat
     7021 atcaaatatt aaattcttct tgcttccttt tccaactatt ctaaaatcat gcatagattt
     7081 tacaagacta tttttcttaa gtatttcttc aagttctttt ttggctgaca taacttcttt
     7141 atcttcaaga catattgggt ccatgtgaat tactagatat atatctaatt tttcagatat
     7201 ttctctctct gcagtatcta taatttcatg tatttccatt atatctatgt tggatggaat
     7261 ttctgcatgt atagatgcaa tacatctacc aactccatag ttatgaataa ttaaatcatg
     7321 tgtacctatt atatatttat aagataacaa tagctctata atagaattta ccatttcttc
     7381 atccggtgct tcccctaaaa gaggatttat agtatctttt acaagagaga aaccagcata
     7441 aagtatcact aatgatacta cgataccaac atatccatct atgggaaaat tagtaaatct
     7501 agctacaata aatgatataa ctacacaact tgaagtaaat acatctccta aagcatcaag
     7561 tgaaactgct tttaaggctg aagaatcaat tttatttccc ataaatttat taaaacgact
     7621 caaccagatt ttaatcatta tagatgcaat taaaagtata agtggtatta cttcaaattt
     7681 tacaggaatt ggatttacta tcctttctat agatgacttt acaaattgaa gtccaaccaa
     7741 catcaccata aatgctacta ttaatgctga taaatactct attcttccat gaccaaaagg
     7801 gtgttcttta tcagctggct tacttgctaa tttaaaacct atcatagtta ttgctgaaga
     7861 tgccatatca gaaagattat taaatgcatc agccattata gctatactag atgtgagcat
     7921 tcctatgaat acctttatca caaataaaag taaattggat aatataccta ctacaccagc
     7981 tacataacca tacttatttc taacatctgt attttgaacg ttttcgctat ctctgataaa
     8041 tgttttgact aggattttgg aaaacatgaa acttacctcc ctgcccttta ttttattaac
     8101 aaattttata atctatatct ttatttatga taacataatt tatatataaa attcttaaaa
     8161 atcactctac ttcagtaaaa tattattcta tatatctatt tagatacgaa ttttatattt
     8221 agtaaagttt ttatggctta gtgtctgttt aatagattct atataagttt ttacaaagat
     8281 tatttattat gataatatca tttttcccaa gtattactta gaatatgatt tttttgtttt
     8341 gtctgtacat attttaaatt tatactatat ttatctatat tttataacct tatgatacac
     8401 tataactata agattttaaa tggaaaggaa tgaaataaat catgtttttt atcagtgata
     8461 tttacacaaa atcacctatt aaatttgata ctcctttgca aaaagaggca tataaaatat
     8521 tacaaaaatt agatattgac tttgaatgtg ttgacacaga tgaagcaatt acaatggaag
     8581 attgtgttca gattaataaa aaattaaata tgaaaatggt aatataaaac aacattaaac
     8641 aaacaaaaac cgcaaagtta caatatatag tactttacgg tttcataaga attaattttt
     8701 actattaaag ttcatagttc tactattttt cattgttcta agaattagct ttacaaattc
     8761 atttgacaat aggatagtgg atgcaaatag tattatcttt atccacatat caaagtccaa
     8821 agaaacagaa ttaaagaaac tctggaacac ttgtgtaaat attatttgaa ttatccctgt
     8881 aactactatt atttgcaatg ccagtttatt tttaaagaaa tttggtattg tactatttaa
     8941 accaaactct ctacaattta atgcattaaa aagggcactg aatgcaaata aagaaaatat
     9001 aacagtacct tgttcttctg atgtagctcc caatatatta aaagctgatt gagtaaatac
     9061 aattgtaatt ataagaattg catttataat aatatttaca aacatagacc tagctattat
     9121 tccagaatgt ctatttattg gttttctttt caatacataa tctctaacag gttctaatcc
     9181 taatgctagg gctggaggac catccattat tatatttacc cataataatt gtattgtagt
     9241 aaatggcata tccttaccag taagttgaga tattatagca attataaatg ctactatatt
     9301 tacagtaagc tgaaactgta taaatctttg aaagttctca tatatacctc ttccccattt
     9361 tattccctct actatagtac taaagctatc atcagtcaat attatatctg ctgcattttt
     9421 agatacttct gttcctgaaa tacccattgc aattccaaca tctgcttgac taagtgctgg
     9481 ggcatcattt ataccatcac ctgttacagc tacaacttcg ccactttttt gaagtgctga
     9541 aactattctc atttttgtat ctggtttact tcttgcaaca atagatattc ctttgatttc
     9601 ttctctcaat tctttatctg tcaatacatc tatatatgtt gcttcaactg cctttttccc
     9661 atcatttaat aatcctaatt catttccaat agctgttgct gtatttatat tatctcctgt
     9721 aagcattttt acatctactc ctgcattaaa tgctttatca atggaatctt tgaccccttc
     9781 tctcaatggg tccactattc ctacaaatcc actaaaaact aaattatctt ccttcatata
     9841 acttcttgaa tttcctatta aattcaattc accttctgaa gtcattgcaa cttctgtttt
     9901 agaattactc atttttttat aagcaaatcc taatgttctc atagatttaa tttgtaactt
     9961 cttaatctca tctaaaatac ttttttctac ttttggagtt attggaacaa tattttttcc
    10021 ttgttgtaca taagaacatt tttttaataa tacttctgga gcaccttttg aaagtaatat
    10081 atctgaatcc tcttgacgaa ttaatgttga catcttcttt tcttctgaac taaatggtat
    10141 ttgtgatatc aaatacgttt gttttctcat ttcattatag ttcttatcat tatgatataa
    10201 aagtaatgca cactctgttg cgcttcctat atatttaaat gaattatctt ctttttctat
    10261 atctgcagtt gaatttacta tacagttttg ttcaaaatag ctattacttt gatactctcc
    10321 tccacttata tatttaccat caacatatgc tacctctacc atcattttgt tttgtgttaa
    10381 agtccctgtt ttgtcggaac atattacaga aacagaacct atagtttcac aggcttcttt
    10441 ctttgttact aatgcattta tctttgccat cttttgcatt gttatagcta aggtaatatt
    10501 tatcatagtt ggcaaacctt ctggaacagt tgcaactatt aatgcaatac aaaccatata
    10561 tgcattttta gcaggttcta atgattgtaa gaaagataat atactgcttg tatctaaaat
    10621 taacatacct tttctctgca tctgtataat cataaacata cacaataaag tagctattat
    10681 gcctgatatt cctgctattt tagcacccaa cttccctaat ttaatttgaa gtggagtttg
    10741 ctggtcttca tttgataaat tctgtgctat cttacccatc tctgtcttat caccagtatg
    10801 agtgacaacc attatgcccc taccatatgc tacaagagtc cctccaaata ccatattgac
    10861 ttgctttgca ggtattgcat cctgttcgat tatttctgtt ttagaataaa ctacttccat
    10921 atctaataca gcatcagcat tcttagctac atcatcagat tctcctgtta gcatatcttc
    10981 tctaagtttc aaatttatag actgtataag tcttccatct gctggtatca tatccccagt
    11041 ttctatatat acaatatctc ctggtactaa atcatttttt gatattttaa ttatcttccc
    11101 atttctaagc acctttactt ctatattttc tgttaatttt gacagggcat gagctgcttt
    11161 tttagatttt ccttcagtta ttatgcctat agaaattcca ataaaaatag cccctaaaat
    11221 acctaatgcg tcatgtaatt ctcccacaaa agaacttatt actgctgctc ctatgagaat
    11281 taatatcata ggttctgtca aactttctcc caactcgtcc caaaatgttc taccttcttt
    11341 tatagtaaat tcatttaatc catatctaag cttcctttct tctacctcat tatcatcaag
    11401 tccaatttct ggatttgttt ttaagtattt taatacttct ttggtaggtt tattataata
    11461 cctcataact ttatccccct tgttaattat ctataaggaa ttatatgctt atgatagctt
    11521 gtatattact tttcaaaaaa aataaggtgt tgattaatca acaccttata gtggttatat
    11581 attattaaat tttatgttac aggctttata aattgtgagc tatacaaatc aaaatattta
    11641 atcttcaaaa tacccttctg gatataatgg aaacttagat ataagttctt gtgcctctgc
    11701 tttacattca tctaatactt ctttattatc aatattttct gctaccttat tcattatccc
    11761 tgctatctgt ataacttctt tttctttcag acctctttga gatattgatg taagacctat
    11821 acgaacacca ctagttacaa atggtgattg tgggtcgtca ggtataacat ttttattaac
    11881 tgttattcct acatattcaa gagctttttc aaattgtttt cctgtaagat tcttacttct
    11941 taagtcaacc attactatat gattatcagt acctccactt acaatactaa aaccatgtga
    12001 aatcagttca gaagctagag tctgagcatt tactacaact tgctccatta tctttttaaa
    12061 tttatctgta cttaaatata atagtgaaaa ggcttttgct gcaactgtat taagatgtat
    12121 agaacctaat gttcctggaa aaacaccttt atcgagtttc tttgcatgtt ctgctttaca
    12181 aagtacaata ccacttcttg gtccacagaa agtctttgtc gtagatgaag aaacaaaatc
    12241 tgcataaggt acaggactag gtattacttt cgctgcgact aatcctgcaa catgtgccat
    12301 atctaccata aagtaagctc ctacttcttt tgcaaccttt gatattcttt cataatctat
    12361 taatcttgga tatgaacttg cccctgcaat tataagtttt ggtctacatt cttttgcctt
    12421 tgcttcaaga gcatcataat ctatacgtcc agtatttggg tctaccccat aaaattcata
    12481 tttataaatg tcatgtaaaa aattaactgc gctaccatgt gtcaagtgtc caccttggtc
    12541 aagacgcata cttaaaacag tgtcatttgg ttttaatatt gatgaatata cacaatagtt
    12601 agctgtagaa cctgaatacg gttgtacgtt tacatgttct gctccaaata cttcttttgc
    12661 acgttttatt gctagggttt caagtttatc tgcttcttca gaacctgctt gaaaacgagc
    12721 ccctggatag ccttcttctg ttttatttgt aaatacacat ccacttaatt caagtacttc
    12781 tgttggtgct gtactttcag aagctatcat ctcaatattg tgttcttgtc taaccaactc
    12841 atcagctaca atcttgtata actctgggtc tgaaattttt gttgttttta acataataat
    12901 cctcctctta attaaagaat atattttatt agtttaatct tcattgataa atttattata
    12961 acggtctata aaacgtaaga tattcctcca ccaggtccta atggtatgcc aatacccatg
    13021 aatagtaaca atattattat ttctattaat gtaaatacca ctgagtatgg aatcatattc
    13081 gatatgacag tacctatacc tatatcttta tcatatcttc tggcaaaggc caatataact
    13141 ggaaaatacg gaaaaagtgg acttattgga tttgttgaag catctccaat acgataagct
    13201 atttgagtta atgctggatt aaagtctagt agcataaaca tgggaacaaa tataggtgct
    13261 aaaatcgccc attttgcaga agcacttcct acaaataaat tgataaatcc agatagcaat
    13321 ataaatccta ttatcaatcc atagctaggt attcccgact ctgctagtaa ttttgctcct
    13381 gttattgata aaattgttcc taagttacta agattaaata aattaataaa ctgggatgct
    13441 ataaatgcta ggactatata tccacccata tcactcatac tactacccat catagatact
    13501 aaatctttgt cactttttat cttcttactt acttttccat acactaatcc tggagttaaa
    13561 aatataatag tgattatagg aactatacct ttcattaaag gtgcattact agatagtata
    13621 gaaccagtct ctggatcttt cataaatgca ttgtcaccta tacataaggc aactatcact
    13681 actactacaa ctaacaatga taataaagcg tattttatac ccttattttc aagttcactc
    13741 aattcctgta tatccttatc atttgttgaa tcattatatt ttgtaaaacg aggagctata
    13801 aatttttcag ttacaaatgc tcctgcaaaa gttaaaacta tcgctgatat aaaagcaaaa
    13861 taaagattca ttgctggatt gccttggtaa gatgggtcca tcatttgagc tgctggtatt
    13921 gtaaatccag ctaacaatac atctgtagta ctaagcataa catttgctgc aaatccccct
    13981 gcaacacctg caaaagctgc aaacatacct acaagaggat gtcttccaat tcctaaaaac
    14041 acaagtgctg caagtggtgg taatattata aatcctacat ctgctgctgt atgtgacaac
    14101 atacctgcaa atacgattgc tacagttaca agtttttgtg gaactttact tatacccttt
    14161 ttcattaaca cttccataaa tccagatttc tcagcaacac ctgctccaag cattgtaacc
    14221 agtactaatc ctaaaggagc aaatgactga aagtttgtaa ctatgctacc taaatatgat
    14281 tgaatctgct ctttctctag tagatttact acctttacaa cttcttttgt agaaggatgt
    14341 atttcttcta ctcctaaatt agatataact gctgacaata ttacaacaat tatacatagt
    14401 aataaaaata tggttactgg gtctggaagt ttatttcctg ctctctctac aacatttagt
    14461 gccctcatca ataatccact atctttgttt ttttcctgtg aattaatttt cctcaaacta
    14521 ataccccctc ataaacttta tctccagaat gctctatctt aaattttatt ttttaattta
    14581 tccatataat ttataacaga ctgacatagt acatttgtac ctaccaatat agaattttta
    14641 tcagctttaa atttaggatt atgccactga tatatacaat cttgttcttt gttgcctacc
    14701 cctaaccagt aaaaaaatcc tggggctttt tccatgaata tagaaaaatc ttcccctcct
    14761 gtagaaggta ttggatcaac tatagcatct tctgaataca aatcacacac agacttacaa
    14821 gctattgcgt acatctcttt actatttata acagctggta aatcaaataa ataatctagc
    14881 tttgcagttg cttgatatcc acgagctatc tcacaaacta tattttctaa attttctgat
    14941 attttctttc gtacttcatt actaaagctt ctacatgtgc ctgtcatatc taccttttct
    15001 gaaattacat tatttgcagt ccctccattt atagtaccta ttgatataac agctgattct
    15061 aatggactta tgtttctgct tactatggtt tgaataccca ttataattgc acttgatgct
    15121 actatagggt ctattgttct gttgggtata cctccgtgac ctccaaatcc atttacttct
    15181 atcttaatag tgtctactgc tgccattaac ccacctaact taacacctat ttttccacaa
    15241 ggtatatctg gatgattatg aagtccaaat atagcatcaa cttttggatt ttccattact
    15301 cctcgttcca ctagcatctt cgcaccttta ttgacttctt cggctggttg aaatataaac
    15361 tttacatttc catgaagact atctctaata tgagacaata catatgcaca tcctacaagg
    15421 caagacgtgt gtatatcatg cccacatgca tgcattatac catcatatct ggatttatac
    15481 ttaatatcaa cctcttcata tattggcaat gcatcaatat ctcctcttag tgctactgtg
    15541 ggaccatcat acttcccttt taataatcca acaactcctg tttccattcc taaatctaat
    15601 atttctatgt ctaaactcac taataaatct tttatagctt ttgtagtcct aaattcttta
    15661 aaacttaatt cggggtgcat atgaatatct gtaaaaaaat ctaaaatatc ttctttatac
    15721 ttttctgaga ttaaaataag ttcatgcata ctaacacctc tccaaaattt tattttttta
    15781 ttttttataa tttaactaca aattatataa gttaataatc tttataaaga tatttttt
//
LOCUS       DAYAPA010000099        15790 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.099, whole genome shotgun sequence.
ACCESSION   DAYAPA010000099 DAYAPA010000000
VERSION     DAYAPA010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15790
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.099"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            <1..97
                     /locus_tag="ACGI59_002822"
     CDS             <1..97
                     /locus_tag="ACGI59_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFG9740601.1"
                     /translation="VKLKISRQAVNKAKNRAFKKIKKDYENYFNL"
     gene            415..7515
                     /gene="tcdB"
                     /locus_tag="ACGI59_002823"
                     /gene_synonym="toxB"
     CDS             415..7515
                     /gene="tcdB"
                     /locus_tag="ACGI59_002823"
                     /gene_synonym="toxB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_074049122.1"
                     /note="Toxigenic strains produce this toxin (TcdB) or
                     TcdA. TcdB-producing strains are tumorigenic in mice;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin TcdB"
                     /protein_id="HFG9740602.1"
                     /translation="MSLVNRKQLEKMANVRFRTQEDEYVAILDALEEYHNMSENTVVE
                     KYLKLKDINSLTDIYIDTYKKSGRNKALKKFKEYLVTEVLELKNNNLTPVEKNLHFVW
                     IGGQINDTAINYINQWKDVNSDYNVNVFYDSNAFLINTLKKTVVESAINDTLESFREN
                     LNDPRFDYNKFFRKRMEIIYDKQKNFINYYKAQREENPELIIDDIVKTYLSNEYSKEI
                     DELNTYIEESLNKITQNSGNDVRNFEEFKNGESFNLYEQELVERWNLAAASDILRISA
                     LKEIGGMYLDVDMLPGIQPDLFESIEKPSSVTVDFWEMTKLEAIMKYKEYIPEYTSEH
                     FDMLDEEVQSSFESVLASKSDKSEIFSSLGDMEASPLEVKIAFNSKGIINQGLISVKD
                     SYCSNLIVKQIENRYKILNNSLNPAISEDNDFNTTTNTFIDSIMAEANADNGRFMMEL
                     GKYLRVGFFPDVKTTINLSGPEAYAAAYQDLLMFKEGSMNIHLIEADLRNFEISKTNI
                     SQSTEQEMASLWSFDDARAKAQFEEYKRNYFEGSLGEDDNLDFSQNIVVDKEYLLEKI
                     SSLARSSERGYIHYIVQLQGDKISYEAACNLFAKTPYDSVLFQKNIEDSEIAYYYNPG
                     DGEIQEIDKYKIPSIISDRPKIKLTFIGHGKDEFNTDIFAGFDVDSLSTEIEAAIDLA
                     KEDISPKSIEINLLGCNMFSYSINVEETYPGKLLLKVKDKISELMPSISQDSIIVSAN
                     QYEVRINSEGRRELLDHSGEWINKEESIIKDISSKEYISFNPKENKITVKSKNLPELS
                     TLLQEIRNNSNSSDIELEEKVMLTECEINVISNIDTQIVEERIEEAKNLTSDSINYIK
                     DEFKLIESISDALCDLKQQNELEDSHFISFEDISETDEGFSIRFINKETGESIFVETE
                     KTIFSEYANHITEEISKIKGTIFDTVNGKLVKKVNLDTTHEVNTLNAAFFIQSLIEYN
                     SSKESLSNLSVAMKVQVYAQLFSTGLNTITDAAKVVELVSTALDETIDLLPTLSEGLP
                     IIATIIDGVSLGAAIKELSETSDPLLRQEIEAKIGIMAVNLTTATTAIITSSLGVASG
                     FSILLVPLAGISAGIPSLVNNELVLRDKATKVVDYFKHVSLVETEGVFTLLDDKIMMP
                     QDDLVISEIDFNNNSIVLGKCEIWRMEGGSGHTVTDDIDHFFSAPSITYREPHLSIYD
                     VLEVQKEELDLSKDLMVLPNAPNRVFAWETGWTPGLRSLENDGTKLLDRIRDNYEGEF
                     YWRYFAFIADALITTLKPRYEDTNIRINLDSNTRSFIVPIITTEYIREKLSYSFYGSG
                     GTYALSLSQYNMGINIELSESDVWIIDVDNVVRDVTIESDKIKKGDLIEGILSTLSIE
                     ENKIILNSHEINFSGEVNGSNGFVSLTFSILEGINAIIEVDLLSKSYKLLISGELKIL
                     MLNSNHIQQKIDYIGFNSELQKNIPYSFVDSEGKENGFINGSTKEGLFVSELPDVVLI
                     SKVYMDDSKPSFGYYSNNLKDVKVITKDNVNILTGYYLKDDIKISLSLTLQDEKTIKL
                     NSVHLDESGVAEILKFMNRKGNTNTSDSLMSFLESMNIKSIFVNFLQSNIKFILDANF
                     IISGTTSIGQFEFICDENDNIQPYFIKFNTLETNYTLYVGNRQNMIVEPNYDLDDSGD
                     ISSTVINFSQKYLYGIDSCVNKVVISPNIYTDEINITPVYETNNTYPEVIVLDANYIN
                     EKINVNINDLSIRYVWSNDGNDFILMSTSEENKVSQVKIRFVNVFKDKTLANKLSFNF
                     SDKQDVPVSEIILSFTPSYYEDGLIGYDLGLVSLYNEKFYINNFGMMVSGLIYINDSL
                     YYFKPPVNNLITGFVTVGDDKYYFNPINGGAASIGETIIDDKNYYFNQSGVLQTGVFS
                     TEDGFKYFAPANTLDENLEGEAIDFTGKLIIDENIYYFDDNYRGAVEWKELDGEMHYF
                     SPETGKAFKGLNQIGDYKYYFNSDGVMQKGFVSINDNKHYFDDSGVMKVGYTEIDGKH
                     FYFAENGEMQIGVFNTEDGFKYFAHHNEDLGNEEGEEISYSGILNFNNKIYYFDDSFT
                     AVVGWKDLEDGSKYYFDEDTAEAYIGLSLINDGQYYFNDDGIMQVGFVTINDKVFYFS
                     DSGIIESGVQNIDDNYFYIDDNGIVQIGVFDTSDGYKYFAPANTVNDNIYGQAVEYSG
                     LVRVGEDVYYFGETYTIETGWIYDMENESDKYYFNPETKKACKGINLIDDIKYYFDEK
                     GIMRTGLISFENNNYYFNENGEMQFGYINIEDKMFYFGEDGVMQIGVFNTPDGFKYFA
                     HQNTLDENFEGESINYTGWLDLDEKRYYFTDEYIAATGSVIIDGEEYYFDPDTAQLVI
                     SE"
     gene            7637..8137
                     /gene="tcdE"
                     /locus_tag="ACGI59_002824"
     CDS             7637..8137
                     /gene="tcdE"
                     /locus_tag="ACGI59_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin-like glycosylating toxin export protein
                     TcdE"
                     /protein_id="HFG9740603.1"
                     /translation="MHSSSPFYISNGNKIFFYINLGGVMNMTISFLSEHIFIKLVILT
                     ISFDTLLGCLSAIKSRKFNSSFGIDGGIRKVAMIACIFFLSVVDILTKFNFLFMLPQD
                     CINFLRLKHLGISEFFSILFILYESVSILKNMCLCGLPVPKRLKEKIAILLDAMTDEM
                     NAKDEK"
     gene            8246..8377
                     /locus_tag="ACGI59_002825"
     CDS             8246..8377
                     /locus_tag="ACGI59_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9740604.1"
                     /translation="MPRDTQVLNTYNFEASVHYYIDDKVVYQTLVHKDGAWSVGKIY"
     gene            8442..8648
                     /locus_tag="ACGI59_002826"
     CDS             8442..8648
                     /locus_tag="ACGI59_002826"
                     /inference="COORDINATES: protein motif:HMM:NF022413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BhlA/UviB family holin-like peptide"
                     /protein_id="HFG9740605.1"
                     /translation="MKLFKNTKIRDEGQNDIENKYQTLINDLIDSLKVIGKIYKEIKT
                     LKKYKICLQITIRQSPYLIEESIN"
     gene            8865..>15790
                     /gene="tcdA"
                     /locus_tag="ACGI59_002827"
                     /gene_synonym="toxA"
     CDS             8865..>15790
                     /gene="tcdA"
                     /locus_tag="ACGI59_002827"
                     /gene_synonym="toxA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_105161403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin TcdA"
                     /protein_id="HFG9740606.1"
                     /translation="MSLISKEELIKLAYSIRPRENEYKTILTNLDEYNKLTTNNNENK
                     YLQLKKLNESIDVFMNKYKTSSRNRALSNLKKDILKEVILIKNSNTSPVEKNLHFVWI
                     GGEVSDIALEYIKQWADINAEYNIKLWYDSEAFLVNTLKKAIVESSTTEALQLLEEEI
                     QNPQFDNMKFYKKRMEFIYDRQKRFINYYKSQINKPTVPTIDDIIKSHLVSEYNRDET
                     VLESYRTNSLRKINSNHGIDIRANSLFTEQELLNIYSQELLNRGNLAAASDIVRLLAL
                     KNFGGVYLDVDMLPGIHSDLFKTISRPSSIGLDRWEMIKLEAIMKYKKYINNYTSENF
                     DKLDQQLKDNFKLIIESKSEKSEIFSKLENLNVSDLEIKIAFALGSVINQALISKQGS
                     YLTNLVIEQVKNRYQFLNQHLNPAIESDNNFTDTTKIFHDSLFNSATAENSMFLTKIA
                     PYLQVGFMPEARSTISLSGPGAYASAYYDFINLQENTIEKTLKASDLIEFKFPENNLS
                     QLTEQEINSLWSFDQASAKYQFEKYVRDYTGGSLSEDNGVDFNKNTALDKNYLLNNKI
                     PSNNVEEAGSKNYVHYIIQLQGDDISYEATCNLFSKNPKNSIIIQRNMNESAKSYFLS
                     DDGESILELNKYRIPERLKNKEKVKVTFIGHGKDEFNTSEFARLSVDSLSNEISSFLD
                     TIKLDISPKNVEVNLLGCNMFSYDFNVEETYPGKLLLSIMDKITSTLPDVNKNSITIG
                     ANQYEVRINSEGRKELLAHSGKWINKEEAIMSDLSSKEYIFFDSIDNKLKAKSKNIPG
                     LASISEDIKTLLLDASVSPDTKFILNNLKLNIESSIGDYIYYEKLEPVKNIIHNSIDD
                     LIDEFNLLENVSDELYELKKLNNLDEKYLISFEDISKNNSTYSVRFINKSNGESVYVE
                     TEKEIFSKYSEHITKEISTIKNSIITDVNGNLLDNIQLDHTSQVNTLNAAFFIQSLID
                     YSSNKDVLNDLSTSVKVQLYAQLFSTGLNTIYDSIQLVNLISNAVNDTINVLPTITEG
                     IPIVSTILDGINLGAAIKELLDEHDPLLKKELEAKVGVLAINMSLSIAATVASIVGIG
                     AEVTIFLLPIAGISAGIPSLVNNELILHDKATSVVNYFNHLSESKKYGPLKTEDDKIL
                     VPIDDLVISEIDFNNNSIKLGTCNILAMEGGSGHTVTGNIDHFFSSPSISSHIPSLSI
                     YSAIGIETENLDFSKKIMMLPNAPSRVFWWETGAVPGLRSLENDGTRLLDSIRDLYPG
                     KFYWRFYAFFDYAITTLKPVYEDTNIKIKLDKDTRNFIMPTITTNEIRNKLSYSFDGA
                     GGTYSLLLSSYPISTNINLSKDDLWIFNIDNEVREISIENGTIKKGKLIKDVLSKIDI
                     NKNKLIIGNQTIDFSGDIDNKDRYIFLTCELDDKISLIIEINLVAKSYSLLLSGDKNY
                     LISNLSNTIEKINTLGLDSKNIAYNYTDESNNKYFGAISKTSQKSIIHYKKDSKNILE
                     FYNDSTLEFNSKDFIAEDINVFMKDDINTITGKYYVDNNTDKSIDFSISLVSKNQVKV
                     NGLYLNESVYSSYLDFVKNSDGHHNTSNFMNLFLDNISFWKLFGFENINFVIDKYFTL
                     VGKTNLGYVEFICDNNKNIDIYFGEWKTSSSKSTIFSGNGRNVVVEPIYNPDTGEDIS
                     TSLDFSYEPLYGIDRYINKVLIAPDLYTSLININTNYYSNEYYPEIIVLNPNTFHKKV
                     NINLDSSSFEYKWSTEGSDFILVRYLEESNKKILQKIRIKGILSNTQSFNKMSIDFKD
                     IKKLSLGYIMSNFKSFNSENELDRDHLGFKIIDNKTYYYDEDSKLVKGLININNSLFY
                     FDPIEFNLVTGWQTINGKKYYFDINTGAALTSYKIINGKHFYFNNDGVMQLGVFKGPD
                     GFEYFAPANTQNNNIEGQAIVYQSKFLTLNGKKYYFDNDSKAVTGWRIINNEKYYFNP
                     NNAIAAVGLQVIDNNKYYFNPDTAIISKGWQTVNGSRYYFDTDTAIAFNGYKTIDGKH
                     FYFDSDCVVKIGVFSTSNGFEYFAPANTYNNNIEGQAIVYQSKFLTLNGKKYYFDNNS
                     KAVTGWQTIDSKKYYFNTNTAEAATGWQTIDGKKYYFNTNTAIASTGYTIINGKHFYF
                     NTDGIMQIGVFKGPNGFEYFAPANTDANNIEGQAILYQNEFLTLNGKKYYFGSDSKAV
                     TGWRIINNKKYYFNPNNAIAAIHLCTINNDKYYFSYDGILQNGYITIERNNFYFDANN
                     ESKMVTGVFKGPNGFEYFAPANTHNNNIEGQAIVYQNKFLTLNGKKYYFDNDSKAVTG
                     WQT"
BASE COUNT         6360 a         1677 c         2493 g         5260 t
ORIGIN      
        1 agtgaaatta aaaatatcta gacaagctgt taataaggct aaaaatagag catttaaaaa
       61 aataaaaaaa gactatgaaa attattttaa cttgtaatta atgagtttaa agaaatattt
      121 acaatagaaa tcaaatttta taattaactt tattgtaaaa ttaataactt aatctaagaa
      181 tatcttaatt tttatatttt atatagaaca atgtttacat atttatttca gacaacgtct
      241 ttattcaatc gaagagcaaa ttaatcaact gagtgtctta aatttaaaat gttaggaagt
      301 gaatgtatat gaaaacctaa gtagatatta gtatatttta taaatagaaa ggaggatata
      361 taaaagagtt ttagcattta gatgtaaaaa tattatagta aaggagaaaa ttttatgagt
      421 ttagttaata gaaaacagtt agaaaaaatg gcaaatgtaa gatttcgtac tcaagaagat
      481 gaatatgttg caatattgga tgctttagaa gaatatcata atatgtcaga gaatactgta
      541 gtcgaaaaat atttaaaatt aaaagatata aatagtttaa cagatattta tatagataca
      601 tataaaaaat ctggtagaaa taaagcctta aaaaaattta aggaatatct agttacagaa
      661 gtattagagc taaagaataa taatttaact ccagttgaga aaaatttaca ttttgtttgg
      721 attggaggtc aaataaatga cactgctatt aattatataa atcaatggaa agatgtaaat
      781 agtgattata atgttaatgt tttttatgat agtaatgcat ttttgataaa cacattgaaa
      841 aaaactgtag tagaatcagc aataaatgat acacttgaat catttagaga aaacttaaat
      901 gaccctagat ttgactataa taaattcttc agaaaacgta tggaaataat ttatgataaa
      961 cagaaaaatt tcataaacta ctataaagct caaagagaag aaaatcctga acttataatt
     1021 gatgatattg taaagacata tctttcaaat gagtattcaa aggagataga tgaacttaat
     1081 acctatattg aagaatcctt aaataaaatt acacagaata gtggaaatga tgttagaaac
     1141 tttgaagaat ttaaaaatgg agagtcattc aacttatatg aacaagagtt ggtagaaagg
     1201 tggaatttag ctgctgcttc tgacatatta agaatatctg cattaaaaga aattggtggt
     1261 atgtatttag atgttgatat gttaccagga atacaaccag acttatttga gtctatagag
     1321 aaacctagtt cagtaacagt ggatttttgg gaaatgacaa agttagaagc tataatgaaa
     1381 tacaaagaat atataccaga atatacctca gaacattttg acatgttaga cgaagaagtt
     1441 caaagtagtt ttgaatctgt tctagcttct aagtcagata aatcagaaat attctcatca
     1501 cttggtgata tggaggcatc accactagaa gttaaaattg catttaatag taagggtatt
     1561 ataaatcaag ggctaatttc tgtgaaagac tcatattgta gcaatttaat agtaaaacaa
     1621 atcgagaata gatataaaat attgaataat agtttaaatc cagctattag cgaggataat
     1681 gattttaata ctacaacgaa tacctttatt gatagtataa tggctgaagc taatgcagat
     1741 aatggtagat ttatgatgga actaggaaag tatttaagag ttggtttctt cccagatgtt
     1801 aaaactacta ttaacttaag tggccctgaa gcatatgcgg cagcttatca agatttatta
     1861 atgtttaaag aaggcagtat gaatatccat ttgatagaag ctgatttaag aaactttgaa
     1921 atctctaaaa ctaatatttc tcaatcaact gaacaagaaa tggctagctt atggtcattt
     1981 gacgatgcaa gagctaaagc tcaatttgaa gaatataaaa ggaattattt tgaaggttct
     2041 cttggtgaag atgataatct tgatttttct caaaatatag tagttgacaa ggagtatctt
     2101 ttagaaaaaa tatcttcatt agcaagaagt tcagagagag gatatataca ctatattgtt
     2161 cagttacaag gagataaaat tagttatgaa gcagcatgta acttatttgc aaagactcct
     2221 tatgatagtg tactatttca gaaaaatata gaagattcag aaattgcata ttattataat
     2281 cctggagatg gtgaaataca agaaatagac aagtataaaa ttccaagtat aatttctgat
     2341 agacctaaga ttaaattaac atttattggt catggtaaag atgaatttaa tactgatata
     2401 tttgcaggtt ttgatgtaga ttcattatcc acagaaatag aagcagcaat agatttagct
     2461 aaagaggata tttctcctaa gtcaatagaa ataaatttat taggatgtaa tatgtttagc
     2521 tactctatca acgtagagga gacttatcct ggaaaattat tacttaaagt taaagataaa
     2581 atatcagaat taatgccatc tataagtcaa gactctatta tagtaagtgc aaatcaatat
     2641 gaagttagaa taaatagtga aggaagaaga gaattattgg atcattctgg tgaatggata
     2701 aataaagaag aaagtattat aaaggatatt tcatcaaaag aatatatatc atttaatcct
     2761 aaagaaaata aaattacagt aaaatctaaa aatttacctg agctatctac attattacaa
     2821 gaaattagaa ataattctaa ttcaagtgat attgaactag aagaaaaagt aatgttaaca
     2881 gaatgtgaga taaatgttat ttcaaatata gatacgcaaa ttgttgagga aaggattgaa
     2941 gaagctaaga atttaacttc tgactctatt aattatataa aagatgaatt taaactaata
     3001 gaatctattt ctgatgcact atgtgactta aaacaacaga atgaattaga agattctcat
     3061 tttatatctt ttgaggacat atcagagact gatgagggat ttagtataag atttattaat
     3121 aaagaaactg gagaatctat atttgtagaa actgaaaaaa caatattctc tgaatatgct
     3181 aatcatataa ctgaagagat ttctaagata aaaggtacta tatttgatac tgtaaatggt
     3241 aagttagtaa aaaaagtaaa tttagatact acacacgaag taaatacttt aaatgctgca
     3301 ttttttatac aatcattaat agaatataat agttctaaag aatctcttag taatttaagt
     3361 gtagcaatga aagtccaagt ttacgctcaa ttatttagta ctggtttaaa tactattaca
     3421 gatgcagcca aagttgttga attagtatca actgcattag atgaaactat agacttactt
     3481 cctacattat ctgaaggatt acctataatt gcaactatta tagatggtgt aagtttaggt
     3541 gcagcaatca aagagctaag tgaaacgagt gacccattat taagacaaga aatagaagct
     3601 aagataggta taatggcagt aaatttaaca acagctacaa ctgcaatcat tacttcatct
     3661 ttgggggtag ctagtggatt tagtatactt ttagttcctt tagcaggaat ttcagcaggt
     3721 ataccaagct tagtaaacaa tgaacttgta cttcgagata aggcaacaaa ggttgtagat
     3781 tattttaaac atgtttcatt agttgaaact gaaggagtat ttactttatt agatgataaa
     3841 ataatgatgc cacaagatga tttagtgata tcagaaatag attttaataa taattcaata
     3901 gttttaggta aatgtgaaat ctggagaatg gaaggtggtt caggtcatac tgtaactgat
     3961 gatatagatc acttcttttc agcaccatca ataacatata gagagccaca cttatctata
     4021 tatgacgtat tggaagtaca aaaagaagaa cttgatttgt caaaagattt aatggtatta
     4081 cctaatgctc caaatagagt atttgcttgg gaaacaggat ggacaccagg tttaagaagc
     4141 ttagaaaatg atggcacaaa actgttagac cgtataagag ataactatga aggtgagttt
     4201 tattggagat attttgcttt tatagctgat gctttaataa caacattaaa accaagatat
     4261 gaagatacta atataagaat aaatttagat agtaatacta gaagttttat agttccaata
     4321 ataactacag aatatataag agaaaaatta tcatattctt tctatggttc aggaggaact
     4381 tatgcattgt ctctttctca atataatatg ggtataaata tagaattaag tgaaagtgat
     4441 gtttggatta tagatgttga taatgttgtg agagatgtaa ctatagaatc tgataaaatt
     4501 aaaaaaggtg atttaataga aggtatttta tctacactaa gtattgaaga gaataaaatt
     4561 atcttaaata gccatgagat taatttttct ggtgaggtaa atggaagtaa tggatttgtt
     4621 tctttaacat tttcaatttt agaaggaata aatgcaatta tagaagttga tttattatct
     4681 aaatcatata aattacttat ttctggcgaa ttaaaaatat tgatgttaaa ttcaaatcat
     4741 attcaacaga aaatagatta tataggattc aatagcgaat tacagaaaaa tataccatat
     4801 agctttgtag atagtgaagg aaaagagaat ggttttatta atggttcaac aaaagaaggt
     4861 ttatttgtat ctgaattacc tgatgtagtt cttataagta aggtttatat ggatgatagt
     4921 aagccttcat ttggatatta tagtaataat ttgaaagatg tcaaagttat aactaaagat
     4981 aatgttaata tattaacagg ttattatctt aaggatgata taaaaatctc tctttctttg
     5041 actctacaag atgaaaaaac tataaagtta aatagtgtgc atttagatga aagtggagta
     5101 gctgagattt tgaagttcat gaatagaaaa ggtaatacaa atacttcaga ttctttaatg
     5161 agctttttag aaagtatgaa tataaaaagt attttcgtta atttcttaca atctaatatt
     5221 aagtttatat tagatgctaa ttttataata agtggtacta cttctattgg ccaatttgag
     5281 tttatttgtg atgaaaatga taatatacaa ccatatttca ttaagtttaa tacactagaa
     5341 actaattata ctttatatgt aggaaataga caaaatatga tagtggaacc aaattatgat
     5401 ttagatgatt ctggagatat atcttcaact gttatcaatt tctctcaaaa gtatctttat
     5461 ggaatagaca gttgtgttaa taaagttgta atttcaccaa atatttatac agatgaaata
     5521 aatataacgc ctgtatatga aacaaataat acttatccag aagttattgt attagatgca
     5581 aattatataa atgaaaaaat aaatgttaat atcaatgatc tatctatacg atatgtatgg
     5641 agtaatgatg gtaatgattt tattcttatg tcaactagtg aagaaaataa ggtgtcacaa
     5701 gttaaaataa gattcgttaa tgtttttaaa gataagactt tggcaaataa gctatctttt
     5761 aactttagtg ataaacaaga tgtacctgta agtgaaataa tcttatcatt tacaccttca
     5821 tattatgagg atggattgat tggctatgat ttgggtctag tttctttata taatgagaaa
     5881 ttttatatta ataactttgg aatgatggta tctggattaa tatatattaa tgattcatta
     5941 tattatttta aaccaccagt aaataatttg ataactggat ttgtgactgt aggcgatgat
     6001 aaatactact ttaatccaat taatggtgga gctgcttcaa ttggagagac aataattgat
     6061 gacaaaaatt attatttcaa ccaaagtgga gtgttacaaa caggtgtatt tagtacagaa
     6121 gatggattta aatattttgc cccagctaat acacttgatg aaaacctaga aggagaagca
     6181 attgatttta ctggaaaatt aattattgac gaaaatattt attattttga tgataattat
     6241 agaggagctg tagaatggaa agaattagat ggtgaaatgc actattttag cccagaaaca
     6301 ggtaaagctt ttaaaggtct aaatcaaata ggtgattata aatactattt caattctgat
     6361 ggagttatgc aaaaaggatt tgttagtata aatgataata aacactattt tgatgattct
     6421 ggtgttatga aagtaggtta cactgaaata gatggcaagc atttctactt tgctgaaaac
     6481 ggagaaatgc aaataggagt atttaataca gaagatggat ttaaatattt tgctcatcat
     6541 aatgaagatt taggaaatga agaaggtgaa gaaatctcat attctggtat attaaatttc
     6601 aataataaaa tttactattt tgatgattca tttacagctg tagttggatg gaaagattta
     6661 gaggatggtt caaagtatta ttttgatgaa gatacagcag aagcatatat aggtttgtca
     6721 ttaataaatg atggtcaata ttattttaat gatgatggaa ttatgcaagt tggatttgtc
     6781 actataaatg ataaagtctt ctacttctct gactctggaa ttatagaatc tggagtacaa
     6841 aacatagatg acaattattt ctatatagat gataatggta tagttcaaat tggtgtattt
     6901 gatacttcag atggatataa atattttgca cctgctaata ctgtaaatga taatatttac
     6961 ggacaagcag ttgaatatag tggtttagtt agagttgggg aagatgtata ttattttgga
     7021 gaaacatata caattgagac tggatggata tatgatatgg aaaatgaaag tgataaatat
     7081 tatttcaatc cagaaactaa aaaagcatgc aaaggtatta atttaattga tgatataaaa
     7141 tattattttg atgagaaggg cataatgaga acgggtctta tatcatttga aaataataat
     7201 tattacttta atgagaatgg tgaaatgcaa tttggttata taaatataga agataagatg
     7261 ttctattttg gtgaagatgg tgtcatgcag attggagtat ttaatacacc agatggattt
     7321 aaatactttg cacatcaaaa tactttggat gagaattttg agggagaatc aataaactat
     7381 actggttggt tagatttaga tgaaaagaga tattatttta cagatgaata tattgcagca
     7441 actggttcag ttattattga tggtgaggag tattattttg atcctgatac agctcaatta
     7501 gtgattagtg aatagataaa aatatgttaa atatatcctc ttatacttaa atatataaaa
     7561 ataaacaaaa tgatacacta cataaagtgt tctatctaat atgaagattt accaataaaa
     7621 aggtggacta tgatgaatgc acagtagttc acctttttat atttctaatg gtaacaaaat
     7681 atttttttat ataaacctag gaggcgttat gaatatgaca atatcttttt tatcagagca
     7741 tatatttata aagttagtaa ttttaactat atcatttgat acattattag gatgtttaag
     7801 tgcaataaaa agtcgtaaat ttaattctag ttttggaata gatggaggaa tcagaaaagt
     7861 agcaatgata gcatgtatat tttttttatc agtagttgac attcttacaa agtttaactt
     7921 tttatttatg ttaccacaag attgtatcaa ttttttaaga ctaaaacatc ttggaatatc
     7981 tgaatttttc tctattttat ttattttata tgaaagtgta agtatattaa aaaatatgtg
     8041 cttatgtgga ttaccagtac ctaagagatt aaaggaaaaa atagcaattt tactagatgc
     8101 aatgacagat gaaatgaatg ctaaggatga aaagtaagta atggtagata taataaagat
     8161 attaacaaat aaaaagtgtt atccaaataa gaatagctga aagttatcat aattcatgaa
     8221 actaataatg aaaacgaggg agcagatgcc aagagacaca caagtattaa atacatataa
     8281 tttcgaagca agtgttcatt actatataga tgacaaggta gtatatcaaa cattggttca
     8341 caaagatggt gcatggtcag ttggtaaaat ctattaagct acattagtta cagatatcac
     8401 aaactataat agttaaacat agaaatatgt gtaaattgtg attgaaatta ttcaaaaaca
     8461 caaaaatacg tgatgaagga caaaatgata tagaaaataa gtatcaaacc ttaataaatg
     8521 atttaattga tagtttaaaa gttataggaa aaatatataa agaaataaaa acattaaaaa
     8581 aatataagat atgtttacaa attactatca gacaatctcc ttatctaata gaagagtcaa
     8641 ttaactaatt gagtatcttt aaattgaaat gttaggaagt gatttaaata tgaaaactta
     8701 aattataaaa aatcaatatt aatttatttt taaaaaatag aaaggagtgt ataagattta
     8761 ttttcaaagt ttaaaaacaa gaaaatcaat ttaaatttca gaaggaataa atgtggttat
     8821 agaagtggat ttattatcaa aaataataat actaggaggt ttttatgtct ttaatatcta
     8881 aagaagagtt aataaaactc gcatatagca ttagaccaag agaaaatgag tataaaacta
     8941 tactaactaa tttagacgaa tataataagt taactacaaa caataatgaa aataaatatt
     9001 tacaattaaa aaaactaaat gaatcaattg atgtttttat gaataaatat aaaacttcaa
     9061 gcagaaatag agcactctct aatctaaaaa aagatatatt aaaagaagta attcttatta
     9121 aaaattccaa tacaagccct gtagaaaaaa atttacattt tgtatggata ggtggagaag
     9181 tcagtgatat tgctcttgaa tacataaaac aatgggctga tattaatgca gaatataata
     9241 ttaaactgtg gtatgatagt gaagcattct tagtaaatac actaaaaaag gctatagttg
     9301 aatcttctac cactgaagca ttacagctac tagaggaaga gattcaaaat cctcaatttg
     9361 ataatatgaa attttacaaa aaaaggatgg aatttatata tgatagacaa aaaaggttta
     9421 taaattatta taaatctcaa atcaataaac ctacagtacc tacaatagat gatattataa
     9481 agtctcatct agtatctgaa tataatagag atgaaactgt attagaatca tatagaacaa
     9541 attctttgag aaaaataaat agtaatcatg ggatagatat cagggctaat agtttgttta
     9601 cagaacaaga gttattaaat atttatagtc aggagttgtt aaatcgtgga aatttagctg
     9661 cagcatctga catagtaaga ttattagccc taaaaaattt tggcggagta tatttagatg
     9721 ttgatatgct tccaggtatt cactctgatt tatttaaaac aatatctaga cctagctcta
     9781 ttggactaga ccgttgggaa atgataaaat tagaggctat tatgaagtat aaaaaatata
     9841 taaataatta tacatcagaa aactttgata aacttgatca acaattaaaa gataatttta
     9901 aactcattat agaaagtaaa agtgaaaaat ctgagatatt ttctaaatta gaaaatttaa
     9961 atgtatctga tcttgaaatt aaaatagctt tcgctttagg cagtgttata aatcaagcct
    10021 tgatatcaaa acaaggttca tatcttacta acctagtaat agaacaagta aaaaatagat
    10081 atcaattttt aaaccaacac cttaacccag ccatagagtc tgataataac ttcacagata
    10141 ctactaaaat ttttcatgat tcattattta attcagctac cgcagaaaac tctatgtttt
    10201 taacaaaaat agcaccatac ttacaagtag gttttatgcc agaagctcgc tccacaataa
    10261 gtttaagtgg tccaggagct tatgcgtcag cttactatga tttcataaat ttacaagaaa
    10321 atactataga aaaaacttta aaagcatcag atttaataga atttaaattc ccagaaaata
    10381 atctatctca attgacagaa caagaaataa atagtctatg gagctttgat caagcaagtg
    10441 caaaatatca atttgagaaa tatgtaagag attatactgg tggatctctt tctgaagaca
    10501 atggggtaga ctttaataaa aatactgccc tcgacaaaaa ctatttatta aataataaaa
    10561 ttccatcaaa caatgtagaa gaagctggaa gtaaaaatta tgttcattat atcatacagt
    10621 tacaaggaga tgatataagt tatgaagcaa catgcaattt attttctaaa aatcctaaaa
    10681 atagtattat tatacaacga aatatgaatg aaagtgcaaa aagctacttt ttaagtgatg
    10741 atggagaatc tattttagaa ttaaataaat ataggatacc tgaaagatta aaaaataagg
    10801 aaaaagtaaa agtaaccttt attggacatg gtaaagatga attcaacaca agcgaatttg
    10861 ctagattaag tgtagattca ctttccaatg agataagttc atttttagat accataaaat
    10921 tagatatatc acctaaaaat gtagaagtaa acttacttgg atgtaatatg tttagttatg
    10981 attttaatgt tgaagaaact tatcctggga agttgctatt aagtattatg gacaaaatta
    11041 cttccacttt acctgatgta aataaaaatt ctattactat aggagcaaat caatatgaag
    11101 taagaattaa tagtgaggga agaaaagaac ttctggctca ctcaggtaaa tggataaata
    11161 aagaagaagc tattatgagc gatttatcta gtaaagaata catttttttt gattctatag
    11221 ataataagct aaaagcaaag tccaagaata ttccaggatt agcatcaata tcagaagata
    11281 taaaaacatt attacttgat gcaagtgtta gtcctgatac aaaatttatt ttaaataatc
    11341 ttaagcttaa tattgaatct tctattggtg attacattta ttatgaaaaa ttagagcctg
    11401 ttaaaaatat aattcacaat tctatagatg atttaataga tgagttcaat ctacttgaaa
    11461 atgtatctga tgaattatat gaattaaaaa aattaaataa tctagatgag aagtatttaa
    11521 tatcttttga agatatctca aaaaataatt caacttactc tgtaagattt attaacaaaa
    11581 gtaatggtga gtcagtttat gtagaaacag aaaaagaaat tttttcaaaa tatagcgaac
    11641 atattacaaa agaaataagt actataaaga atagtataat tacagatgtt aatggtaatt
    11701 tattggataa tatacagtta gatcatactt ctcaagttaa tacattaaac gcagcattct
    11761 ttattcaatc attaatagat tatagtagca ataaagatgt actgaatgat ttaagtacct
    11821 cagttaaggt tcaactttat gctcaactat ttagtacagg tttaaatact atatatgact
    11881 ctatccaatt agtaaattta atatcaaatg cagtaaatga tactataaat gtactaccta
    11941 caataacaga ggggatacct attgtatcta ctatattaga cggaataaac ttaggtgcag
    12001 caattaagga attactagac gaacatgacc cattactaaa aaaagaatta gaagctaagg
    12061 tgggtgtttt agcaataaat atgtcattat ctatagctgc aactgtagct tcaattgttg
    12121 gaataggtgc tgaagttact attttcttat tacctatagc tggtatatct gcaggaatac
    12181 cttcattagt taataatgaa ttaatattgc atgataaggc aacttcagtg gtaaactatt
    12241 ttaatcattt gtctgaatct aaaaaatatg gccctcttaa aacagaagat gataaaattt
    12301 tagttcctat tgatgattta gtaatatcag aaatagattt taataataat tcgataaaac
    12361 taggaacatg taatatatta gcaatggagg ggggatcagg acacacagtg actggtaata
    12421 tagatcactt tttctcatct ccatctataa gttctcatat tccttcatta tcaatttatt
    12481 ctgcaatagg tatagaaaca gaaaatctag atttttcaaa aaaaataatg atgttaccta
    12541 atgctccttc aagagtgttt tggtgggaaa ctggagcagt tccaggttta agatcattgg
    12601 aaaatgacgg aactagatta cttgattcaa taagagattt atacccaggt aaattttact
    12661 ggagattcta tgcttttttc gattatgcaa taactacatt aaaaccagtt tatgaagaca
    12721 ctaatattaa aattaaacta gataaagata ctagaaactt cataatgcca actataacta
    12781 ctaacgaaat tagaaacaaa ttatcttatt catttgatgg agcaggagga acttactctt
    12841 tattattatc ttcatatcca atatcaacga atataaattt atctaaagat gatttatgga
    12901 tatttaatat tgataatgaa gtaagagaaa tatctataga aaatggtact attaaaaaag
    12961 gaaagttaat aaaagatgtt ttaagtaaaa ttgatataaa taaaaataaa cttattatag
    13021 gcaatcaaac aatagatttt tcaggcgata tagataataa agatagatat atattcttga
    13081 cttgtgagtt agatgataaa attagtttaa taatagaaat aaatcttgtt gcaaaatctt
    13141 atagtttgtt attgtctggg gataaaaatt atttgatatc caatttatct aatactattg
    13201 agaaaatcaa tactttaggc ctagatagta aaaatatagc gtacaattac actgatgaat
    13261 ctaataataa atattttgga gctatatcta aaacaagtca aaaaagcata atacattata
    13321 aaaaagacag taaaaatata ttagaatttt ataatgacag tacattagaa tttaacagta
    13381 aagattttat tgctgaagat ataaatgtat ttatgaaaga tgatattaat actataacag
    13441 gaaaatacta tgttgataat aatactgata aaagtataga tttctctatt tctttagtta
    13501 gtaaaaatca agtaaaagta aatggattat atttaaatga atccgtatac tcatcttacc
    13561 ttgattttgt gaaaaattca gatggacacc ataatacttc taattttatg aatttatttt
    13621 tggacaatat aagtttctgg aaattgtttg ggtttgaaaa tataaatttt gtaatcgata
    13681 aatactttac ccttgttggt aaaactaatc ttggatatgt agaatttatt tgtgacaata
    13741 ataaaaatat agatatatat tttggtgaat ggaaaacatc gtcatctaaa agcactatat
    13801 ttagcggaaa tggtagaaat gttgtagtag agcctatata taatcctgat acgggtgaag
    13861 atatatctac ttcactagat ttttcctatg aacctctcta tggaatagat agatatatca
    13921 ataaagtatt gatagcacct gatttatata caagtttaat aaatattaat accaattatt
    13981 attcaaatga gtactaccct gagattatag ttcttaaccc aaatacattc cacaaaaaag
    14041 taaatataaa tttagatagt tcttcttttg agtataaatg gtctacagaa ggaagtgact
    14101 ttattttagt tagatactta gaagaaagta ataaaaaaat attacaaaaa ataagaatca
    14161 aaggtatctt atctaatact caatcattta ataaaatgag tatagatttt aaagatatta
    14221 aaaaactatc attaggatat ataatgagta attttaaatc atttaattct gaaaatgaat
    14281 tagatagaga tcatttagga tttaaaataa tagataataa aacttattac tatgatgaag
    14341 atagtaaatt agttaaagga ttaatcaata taaataattc attattctat tttgatccta
    14401 tagaatttaa cttagtaact ggatggcaaa ctatcaatgg taaaaaatat tattttgata
    14461 taaatactgg agcagcttta actagttata aaattattaa tggtaaacac ttttatttta
    14521 ataatgatgg tgtgatgcag ttgggagtat ttaaaggacc tgatggattt gaatattttg
    14581 cacctgccaa tactcaaaat aataacatag aaggtcaggc tatagtttat caaagtaaat
    14641 tcttaacttt gaatggcaaa aaatattatt ttgataatga ctcaaaagca gtcactggat
    14701 ggagaattat taacaatgag aaatattact ttaatcctaa taatgctatt gctgcagtcg
    14761 gattgcaagt aattgacaat aataagtatt atttcaatcc tgacactgct atcatctcaa
    14821 aaggttggca gactgttaat ggtagtagat actactttga tactgatacc gctattgcct
    14881 ttaatggtta taaaactatt gatggtaaac acttttattt tgatagtgat tgtgtagtga
    14941 aaataggtgt gtttagtacc tctaatggat ttgaatattt tgcacctgct aatacttata
    15001 ataataacat agaaggtcag gctatagttt atcaaagtaa attcttaact ttgaatggta
    15061 aaaaatatta ctttgataat aactcaaaag cagttaccgg atggcaaact attgatagta
    15121 aaaaatatta ctttaatact aacactgctg aagcagctac tggatggcaa actattgatg
    15181 gtaaaaaata ttactttaat actaacactg ctatagcttc aactggttat acaattatta
    15241 atggtaaaca tttttatttt aatactgatg gtattatgca gataggagtg tttaaaggac
    15301 ctaatggatt tgaatatttt gcacctgcta atacggatgc taacaacata gaaggtcaag
    15361 ctatacttta ccaaaatgaa ttcttaactt tgaatggtaa aaaatattac tttggtagtg
    15421 actcaaaagc agttactgga tggagaatta ttaacaataa gaaatattac tttaatccta
    15481 ataatgctat tgctgcaatt catctatgca ctataaataa tgacaagtat tactttagtt
    15541 atgatggaat tcttcaaaat ggatatatta ctattgaaag aaataatttc tattttgatg
    15601 ctaataatga atctaaaatg gtaacaggag tatttaaagg acctaatgga tttgagtatt
    15661 ttgcacctgc taatactcac aataataaca tagaaggtca ggctatagtt taccagaaca
    15721 aattcttaac tttgaatggc aaaaaatatt attttgataa tgactcaaaa gcagttactg
    15781 gatggcaaac
//
LOCUS       DAYAPA010000100        15789 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.100, whole genome shotgun sequence.
ACCESSION   DAYAPA010000100 DAYAPA010000000
VERSION     DAYAPA010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15789
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.100"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            77..715
                     /locus_tag="ACGI59_002828"
     CDS             77..715
                     /locus_tag="ACGI59_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9740607.1"
                     /translation="MSNQRKDFDITNDMFQESIPITTKIILEDMPSNDELNHVFSKGC
                     ERKMKKRKIVLITLLLIGVLLLGSILYNLFLGKTANISMLKESWNFDIPIPNKEIEVF
                     DTQDSINGDGQSYFIQGFSEKNFKKVFNLKGGIVVSKDNINEIEKYIDKFKRDSVNIN
                     KSNKNKIEEDFKKYKLEVKKDDKYIYKRNYENYVVLIIKKDEQKLYSLIWNQ"
     gene            complement(1188..2546)
                     /gene="rlmD"
                     /locus_tag="ACGI59_002829"
     CDS             complement(1188..2546)
                     /gene="rlmD"
                     /locus_tag="ACGI59_002829"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFG9740608.1"
                     /translation="MLSKNEMYIVDIVDIGQGGVGVGKYNGITVFVEGGLIHDKVKVR
                     INKVKKNYAVGDIVEIIEKSPFRVKRVCSDNLRDCGGCQIQDLDYNKQLEMKTNEVKQ
                     VLSRIGKLEDVEVHDTIGMDNPYRYRNKAQFPIQKNNGIPIIGFYKKKTHDVISTEKC
                     VIQHEINDKIVKIIKTYIRAYKVSIYDEKTHTGLIRHLVTKIGFTTKEVMVVLVANGN
                     KLPYLKELASVLKENIPGFKTLVVNVNQKDTNAILGRENIVIYGDGKINDYIGDLVFE
                     ISPLSFFQVNPVQTKVLYDKALEYANLNDTDTVFDIYCGIGTISLFLAQKAKKVYGIE
                     IIDDAIKDAKINAKLNNIHNAEFYVGKAEEIVPKMYREGKTANVVVVDPPRKGCDEKV
                     LETIVSMNPERIVYVSCNPSTLARDLDYLNVHGFKCVEAQPVDMFPHSTHVETVAKII
                     KQ"
     gene            complement(2721..4481)
                     /gene="pyk"
                     /locus_tag="ACGI59_002830"
     CDS             complement(2721..4481)
                     /gene="pyk"
                     /locus_tag="ACGI59_002830"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFG9740609.1"
                     /translation="MLNNVKKTKIVCTLGPASQSEEVLTQLMQNGLNVCRFNFSHGSH
                     EEHKERIDMAKKVREKLNKPVAILLDTKGPEIRTGNFEDPEVFLEEGQKFTITMKDIM
                     GTKEMCTVSYKGLAEDVKSGDSILIDDGLVGLRVKEINGEDIVCVVENSGIVKNHKGV
                     NVPGVKINLPAITPKDISDIEFGISQGIDYIAASFVRKASDVLAIREVLENNNATDIQ
                     IISKIENQEGVENLDEILKVSDGIMVARGDLGVEIPTEEMPIVQKMMIKKCNELAKPV
                     VTATQMLDSMIRNPRPTRAEVTDVANAIYDGTDAIMLSGETAAGKYPVEAVKMMATIA
                     KRTEETLDYDRLLKENGTNNVTVTDAISHATCTTAVDLNASAIITSTSSGYTARMVSK
                     FRPKSPIIATTNNEKTMNKLALTWGVYPVKSSVAGNTDEVIEKAIEAARQADYIDNGE
                     LVVITAGVPVGVSGTTNLIKVHVISEEIVQGIGVGTQTVEGKVRIIKNGNACVDFNEG
                     DILVSTATDAEMNNYIEKAGAVVTENGGMTSHTAIVGINLDIPVIVSAADITNLVKDG
                     EVVTVDASRGVVYRGSTRVL"
     gene            complement(4533..5492)
                     /gene="pfkA"
                     /locus_tag="ACGI59_002831"
     CDS             complement(4533..5492)
                     /gene="pfkA"
                     /locus_tag="ACGI59_002831"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFG9740610.1"
                     /translation="MKTIGLLTSGGDAPGMNAAIRAVVRSAIYYGCKVYGINRGYKGL
                     LEEDLTEMNLSSVGDIIHRGGTILKSSRCEEFKTEEGRLKAVKILKKYKIDCLVVIGG
                     DGSFAGAQKLSDLGFPAIGIPGTIDNDLAYTDYTIGFDTAMNTIIDAIGKIRDTSSSH
                     ERVNIVEVMGRHCGDLALYAGLAGGAETIIVPEVEITVDEVALRLKTTQKRGKRHSII
                     VLAEGVGSASDLEKELKKESGADLRVTVLGHVQRGGSPTVSDRILASRLGVRAVELLL
                     DGKSARVVGIKENKIIDLEISEALAQKKVFDKEAYEMAKILSI"
     gene            complement(5701..9270)
                     /locus_tag="ACGI59_002832"
     CDS             complement(5701..9270)
                     /locus_tag="ACGI59_002832"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFG9740611.1"
                     /translation="MNKDFAHLHVHTEYSLLDGFSRVKKLIKRAKELNMSSIAITDHG
                     CMFGVIDFYKTAIKEGIKPIIGCEVYTAARGLRDKDPNYDKYQGHLVLLAKNMEGYKN
                     LIKIVSTSYVEGFYYKPRVDMEELKKHNKGIIALSACLAGDVARALMNRNYEKAKQFA
                     LDYRDIFGEENFFLEIQDHNLPEQKEVNSGLVKLSKETGIPLVATNDVHYVNKEDSKI
                     HDVLMCIQMGKTLNDPNRMRFGSDEFYLKSREEMEELFPYALEAIDNTVKIADMCNVE
                     FDFNTIHLPKYDVPEGYTPETYLRELCFNGLKERYDNPSNEILERLNYELDVIEKMGY
                     VEYFLIVWDFINFSKENNIIVGPGRGSAAGSIVAYTLKITDIDPIKYSLLFERFLNPE
                     RISMPDIDIDFCYERREEVIDYVKRKYGDDHVAQIITFGTMGAKAAIRDVGRVLDIGY
                     NKVDKVAKEIPFALGMTIDKALDTNPNLKELYDQDPETKEIINISKQIEGMLRHASTH
                     AAGVVISKNPVDEYVPLYKHQDAITTQFTMTTLEELGLLKMDFLGLRTLTVIRDTLDL
                     IEKNRVIKNYTEKIDFSKMDYDDPKVYEMLSSGNTLGVFQLESAGMRSFMKQLKPDNF
                     EDIVAGISLFRPGPMDSIPAYIKNKSNPKDVTYLHEKLKPIMEVTYGCLVYQEQVMQV
                     VRDLGGYSYGRSDLVRRAMSKKKMDVMEEERQYFIHGKFDEDGNIEIAGCIRNGVEEE
                     IANKIFDDMIDFARYAFNKSHAAAYGVLAYETAYLKTYYPVEFMAALITSVMGNTDKV
                     VEYIRECKALEIDVLPPDINKSFSKFSVEGDNIRFGLAAVKNVGVNIINNIIAERESN
                     GLFVDLVDLVKRLDQKDTNKRVIESLIKCGAFDNISENRASLMAGYESLLESVSMDRK
                     KNVQGQISLFDAFGDSEEDSDFQQIYSLPKRQEYEERERLNLEKEVLGMYVSGHPLSQ
                     FEKELQEKTSMDNGKLNSLKEEEESYLQMNETDVIMGGMIVTKTIKTTKRNEIMAFIE
                     LEDLYGAIEIIVFPQLLQKYNVILNEDNIIYVKGTLSIKEGENAKLIAREIKDINDDT
                     DFADRNYNSGRTRNVENIKPESGKKLYLKIDSMSDTDTTNNIVEIAKQYPGNDSIYLY
                     PMDENINGKRKAYKMTGALTCICDEFINNLEELLPKDNIKVKA"
     gene            complement(9427..10374)
                     /gene="whiA"
                     /locus_tag="ACGI59_002833"
     CDS             complement(9427..10374)
                     /gene="whiA"
                     /locus_tag="ACGI59_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HFG9740612.1"
                     /translation="MSFSTETKNELARVISGNECCNIAELSALVKSGGSIQIVGYKKL
                     NLKITTELNSIARKVFKLLKKNFNINTTISVNKNQMLKRNNSYVLMVTSEMGSEMLLK
                     KLGILEDKEGFFTINKISENLIKHEGCKRAFIRGAFLGGGSISDPEKNYHMEFVTNNE
                     EFADSLKELINSLGFNSKIVARKNNHVVYIKESEQISDLLSIIGGHHALLSLQNTKIV
                     KEMRNNVNRIVNCETANLSKTVNAAVRQVENIKLIQETIGISSLPENLQEIAKIRIEY
                     EDMTLKELGEMLEPPIGKSGVNHRLRKIEEIATDLKKKS"
     gene            complement(10391..10816)
                     /locus_tag="ACGI59_002834"
     CDS             complement(10391..10816)
                     /locus_tag="ACGI59_002834"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFG9740613.1"
                     /translation="MREEVSAGGVVLFGNTILLLRKFNGDWVLPKGKVEEGENNQEAA
                     LREVSEETGVKADILKYLGEIHYTFKENWDENRAVHKTVFWYLMQAKNMDTIPQKEEG
                     FIDAKFIHLDRVVDLARYDDEKEIIKVALQEIKKRLKKN"
     gene            complement(10885..11991)
                     /gene="yvcK"
                     /locus_tag="ACGI59_002835"
     CDS             complement(10885..11991)
                     /gene="yvcK"
                     /locus_tag="ACGI59_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFG9740614.1"
                     /translation="MAKLLIIVGILGWIALIVSLFIYKKAKNEKVKLFQRSIVENREP
                     NVVVIGGGTGQSVFLRGLKHTTQNITAIVTVADDGGGSGVLREDLGMLPPGDIRNCLL
                     ALANIEPTMNEVMQYRFTEGLLKGQSFGNLFLAAMNGLYGNFEKAVYKLSEIFAITGR
                     VLPVTLEDVNLVAKLENGNIINGESSIPEESKIQKSSIDKIFLNPKDVKPLKDVIASI
                     YDADIIIMGPGSLYTSIIPNLLVEGIVDAIKSSVAPKVYIANIMTQPGETEGYNVLEH
                     VNAIVKHTDENLIDYVIANNEILPEEMLDLYKQDGAEQVLLDKKQKDKLKEMGIKTVE
                     KNLIEIKNNYIRHDAKYISNIVIELALNHNYNKS"
     gene            complement(12003..12860)
                     /gene="rapZ"
                     /locus_tag="ACGI59_002836"
     CDS             complement(12003..12860)
                     /gene="rapZ"
                     /locus_tag="ACGI59_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFG9740615.1"
                     /translation="MKFVIVTGLSGSGKSETMRALEDMGFYCVDNLPPALITKFAELC
                     YQPNSSIDKVALGIDIRGRKFFEALHESLNYLEKENYEYEMVYLDCNDDVLLKRYKMT
                     RRNHPLAKDMQIPEGIKMERKIMEPLKELSTCIIDTTNMKPKDLKEEIKKIYSSGEDN
                     PNLTISVVSFGFKHGILADADLVFDVRFLPNPYYVEELRAKTGDDKEVRDYVMNSKIS
                     EEFYVKLLDMIHFLVPQYIEEGKQHLVIGVGCTGGRHRSVTITNLIAEDLSNKGYRVV
                     KKHRDSMLR"
     gene            complement(12891..13604)
                     /locus_tag="ACGI59_002837"
     CDS             complement(12891..13604)
                     /locus_tag="ACGI59_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFG9740616.1"
                     /translation="MKILADYHTHTLYSHGKGTIEDNVKVAISKGIKTIGISDHSYKH
                     IAYGVKKKDILKMREEVDYLNDKYTDINILLGMECNILDDKGNIDMDDKFIRDFDYIM
                     AGYHFGSTPTSLRSLLNHCNNYIFKTEKAKEYNTKAVINAISNNDIFIITHPGDKGDV
                     YIEEVAKTAKKTNTRLEINSSHSFLNVNQLNKIKGLENKFIIGSDAHCPERVGDFELA
                     MKTISDSNLDISLVENIII"
     gene            complement(13608..14522)
                     /gene="murB"
                     /locus_tag="ACGI59_002838"
     CDS             complement(13608..14522)
                     /gene="murB"
                     /locus_tag="ACGI59_002838"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFG9740617.1"
                     /translation="MNNQDIYINLLNILSKEDIKVDEPMKKHISFRVGGPADILVRPR
                     TEEQLKNVLKLVKKESIPYLIIGNGSNILIKDGGIRGVVIELADNFNSYEINDTRMTA
                     QSGALLSVLGKALQKQELKGFEFASGIPGTLGGALAMNAGAYGGEMKDIVKSVRLMDM
                     EGNIFELSNEQMEFGYRKSIISKNGYIALSAELELQEGNYDEIKSLMDDLATRRITKQ
                     PLNFASAGSTFKRPTGYFAGKLIEETGLRGLTLRGAQVSEKHCGFVVNQGEASAKDIL
                     DLIYVIKSAVYAKFGVMLEEEVKILGED"
     gene            complement(14795..15565)
                     /locus_tag="ACGI59_002839"
     CDS             complement(14795..15565)
                     /locus_tag="ACGI59_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFG9740618.1"
                     /translation="MSKGFLTPGETAQATISAGIKKANISTQNAILLGLFGGAFIGFG
                     ALGSMIVSQTFGKIDPGVASFLSAMVFPVGLMLVVVAGAELFTGNTLMAIALMDKKIT
                     LGKMLRNWGLVYFANLVGSILLVALVFYSSTLTGDAATKAIAVAEAKVNSTIASMFLK
                     AILCNILVVLAVWMATASQDIMSKLASCWTVIMLFVLCGFQHSVANMFFIPMGMALGA
                     NITMVQFITNLVFVTLGNIVGGSIVVGGIYYLCYVKNS"
BASE COUNT         4861 a         3002 c         1716 g         6210 t
ORIGIN      
        1 tttaaaagag gagaatgtat ataagagtat tcaatgtact agaaaaaatc aaaagaaata
       61 ttagaagaaa tggatattga gtaatcaaag aaaagatttt gatattacaa atgatatgtt
      121 tcaagaaagc attcctatca caacaaaaat tattcttgaa gacatgcctt caaatgatga
      181 attaaatcat gttttttcaa aaggttgtga acgaaaaatg aaaaagagaa aaattgtatt
      241 aatcacacta ctattaatag gtgttttgtt attaggaagt atcctatata atcttttttt
      301 aggaaaaaca gctaatattt caatgctaaa ggaatcttgg aattttgata taccaatacc
      361 taacaaagaa attgaagtat ttgatactca agatagtatt aatggagatg gacaaagtta
      421 ttttattcaa ggattttctg aaaaaaattt caaaaaagtt tttaatttaa aaggtggtat
      481 agttgtaagc aaagataata ttaatgaaat agaaaaatat attgataaat ttaaaagaga
      541 ttctgttaac attaataaaa gtaataaaaa taaaattgaa gaagatttta aaaaatataa
      601 gttagaagtt aaaaaagatg ataagtatat atataagaga aattatgaga actacgtagt
      661 tttaattata aaaaaagatg aacaaaaatt atacagttta atatggaatc agtaggtaca
      721 tatgtattat taagaattat aaataataaa aataaatttt agtcaatata caaaaacctt
      781 ttaaattgtc tggaaatcac ctcttcattt gttataaaaa catacaacaa atgaagaggt
      841 gattttatgg ttttaaaaaa aataattata agtggatatg tgctttcaca tgtttatcaa
      901 catttgtaat gcctatattt gctaatgaat tataaaaata ttaaataata ttcaactata
      961 gattatgtaa attactctat gaaaaaaatg agttgtataa aatctggcta ctgtagattg
     1021 actatttctt ttaaaattac aattataata agttttatag aattaaaaat aaaactttag
     1081 agatttttga taacattgta gactaaattt gccaccgaaa tcgctttaca gtggcaaatc
     1141 ggtggcaaat acaatatatt attagtatat aatctatata aacattacta ttgtttgatt
     1201 atcttagcta cagtttccac atgtgtagaa tgagggaaca tatctacagg ttgtgcctca
     1261 acacatttaa atccgtgaac atttagataa tctaaatccc ttgctaaagt agaaggatta
     1321 caagaaacat agacaattct ttccggattc attgaaacaa tagtctctaa taccttttcg
     1381 tcacatccct ttctaggtgg gtctacaacg actacattgg cagtcttacc ctctctatac
     1441 atcttaggta caatttcctc tgcctttcca acataaaatt cagcattatg tatattgttt
     1501 aatttagcat ttatcttagc atcttttatg gcatcatcta taatttctat tccataaacc
     1561 tttttagcct tttgtgccaa aaataaagat attgtaccta tcccacagta aatatcaaaa
     1621 acagtatcag tatcatttaa atttgcatat tcaagagcct tatcatacaa tactttagtt
     1681 tggacagggt ttacttggaa aaatgataat ggtgaaatct caaacactaa gtctcctata
     1741 taatcgttta tttttccatc tccatatata actatatttt ctcttcctag aatagcattc
     1801 gtatcttttt gatttacatt tacaaccaaa gtcttaaatc caggtatatt ttccttaagc
     1861 actgaagcta attctttaag atatggaagt ttgtttccat ttgctactaa tacaaccatt
     1921 acttctttag ttgtaaaacc tatttttgtt actaaatgcc taatcaaccc cgtgtgagtc
     1981 ttttcatcat atatgcttac cttataggcc cttatatatg tttttattat tttaacaatt
     2041 ttatcattta tctcatgttg tattacgcat ttttctgtcg atattacatc atgagtcttc
     2101 tttttataga agccaataat tggaatacca ttattctttt gtattggaaa ttgtgcttta
     2161 tttctatatc tatatggatt atccattcct atagtgtcat gaacttctac atcctctaac
     2221 ttacctattc tcgaaagtac ttgtttaact tcatttgttt tcatttcaag ttgtttatta
     2281 taatctaagt cttgtatctg acaccctcca caatccctaa gattgtcact acacactctt
     2341 ttcaccctga atggagactt ttctattatc tcaacaatat ctcccacagc ataatttttc
     2401 tttactttat taatcctaac tttaacttta tcatgtatta atccgccctc tacaaaaact
     2461 gtaataccat tgtactttcc tactcctact ccgccttgac caatgtctac tatatctaca
     2521 atatacatct cgtttttcga aagcataaat caccctccaa tcaatctatt atatttaaat
     2581 ctttacaaac ttttttcttt aaatttatat taaaatattg ctattaatta aactatattt
     2641 tttactaagc tacaatattt tattttacca aaaaaaaagg agcttataaa gccccttagt
     2701 aaataaattt attatttttc ttataaaact ctagttgaac ctctatatac cacgcctctt
     2761 gatgcatcca cagttacaac ttctccatct tttactagat ttgttatatc agctgcagac
     2821 acaataactg gtatatctaa atttatacct acaatagctg tatgtgatgt cattccacca
     2881 ttctcagtta ctacagctcc tgctttttct atataattgt tcatttcagc atcagtagca
     2941 gtagaaacaa gtatgtcgcc ttcattaaag tctacacaag catttccatt ctttattata
     3001 cgaactttac cttctactgt ttgagtacct acacctatac cttgcacgat ttcttcgctt
     3061 attacatgaa cctttattaa attagtagtt ccacttactc ctactggtac tccagcagtt
     3121 ataactacta attccccatt atctatataa tcagcttgtc tagctgcctc aattgccttt
     3181 tctataactt catctgtatt tcctgcaaca gaagacttaa ctggatatac tccccatgta
     3241 agagctaatt tattcatagt cttttcatta tttgtagtag ctattattgg agattttggt
     3301 ctgaatttag ataccattct agcagtatat cctgaagaag tagatgttat tatagctgat
     3361 gcatttaaat ctactgctgt tgtacaagtt gcatggctta ttgcatctgt tacagtaaca
     3421 ttgttagtac cattttcctt tagtaatcta tcataatcta aagtctcttc tgttcttttt
     3481 gctattgtag ccatcatttt tactgcctct acaggatatt ttccagcagc agtttctcca
     3541 gataacatta tcgcatctgt tccatcatat atagcattgg caacgtctgt aacttctgct
     3601 cttgttggtc ttggatttct tatcatagaa tcaagcattt gagtggcagt aacaactggc
     3661 tttgctaatt cattacattt ttttatcatc attttttgaa ctattggcat ctcttcagtt
     3721 ggtatctcaa cacctaagtc acctctagcc accattatac catcagaaac ttttaatatt
     3781 tcatctaggt tctctacacc ttcttggttt tctattttag atattatttg tatatcagtt
     3841 gcattattgt tctctaatac ttctcttatt gctagtacat cagatgcctt tcttacaaaa
     3901 gaagcagcta tataatctat tccttgactt attccaaact ctatatcact tatatcttta
     3961 ggagttattg ctggtaaatt tatcttaaca ccaggtacat ttactccttt atgattttta
     4021 actattcctg aattttcaac aacacaaact atatcttctc catttatttc ttttactctt
     4081 aagccaacta aaccatcatc tattagtata gaatctcctg atttaacgtc ttcagcaaga
     4141 cctttatagc ttactgtaca catttcttta gtccccataa tatctttcat agttatagtg
     4201 aacttttgcc cttcttctaa aaatacctct gggtcttcaa agttacctgt tctaatttct
     4261 ggacctttag tatctaataa tatagcaact ggcttattta atttttctct aactttcttt
     4321 gccatatcaa ttctttcttt atgttcttca tgcgaaccgt gagaaaagtt aaatctacac
     4381 acatttagac cattttgcat aagttgagtt aaaacttcct cactttgtga tgctggacct
     4441 agtgtacaaa caatttttgt cttctttaca ttatttaaca tatttgctcc tcctgagata
     4501 ttattccaca gatttatttt taatactaaa acttatatag ataatatttt tgccatctca
     4561 taagcttctt tatcaaatac tttcttttga gctaaagctt cacttatttc taaatcaatt
     4621 attttgtttt cttttattcc tacaactcta gctgattttc catctaataa taattctact
     4681 gctcttactc caagtctact tgctagtatt ctatcagata cagttgggct tcctcctctt
     4741 tggacatgcc ccaatacagt gactcttaaa tctgctcctg actctttttt aagttccttt
     4801 tctaaatcac tagcacttcc gactccttct gctaaaacga ttatactatg tctcttacct
     4861 cttttttgag tagtctttaa tcttagagca acttcatcta ctgtaatttc tacttctggt
     4921 actattatag tttccgctcc tcctgcaaga cctgcatata aggctaaatc tccacagtgt
     4981 cttcccataa cttctactat gttaactctt tcatgagatg atgatgtatc tcttatctta
     5041 cctatagcat ctataatagt attcattgca gtatcaaatc ctatagtata atctgtatat
     5101 gctaaatcgt tatctatagt tcctggtatg cctatagctg ggaatcctaa gtcactaagt
     5161 ttttgggctc ctgcaaatga accatctcca ccaattacaa ctaagcaatc aattttatac
     5221 tttttaagaa tttttactgc ttttaatcta ccttcttctg ttttaaattc ttcacatctg
     5281 gaagatttta atatagttcc tcccctgtgt ataatatctc caacagaaga taagttcatt
     5341 tcagttaaat cttcttctag taatccctta tatcctctat tgattccata tactttgcat
     5401 ccataatata ttgctgatct tacaaccgct ctaatcgcag cattcattcc tggagcatct
     5461 cctccacttg ttagtaatcc tatagttttc attttttacc tccttggatt ttatgtcaat
     5521 tttttattat aatcattgtt attgtattct aatggacttt ttttgtccct atatcacttt
     5581 ttaatcccat caagactaaa aaaattaaat gtccttaaat gtccattttg tgatattata
     5641 tcacaaaacg gacaatattt ttcatattat tataatatat ttagattttt atataaaaaa
     5701 tcaagctttt acttttatgt tatctttagg aagtaactct tctaagttat ttataaattc
     5761 atcacatata caagtcaatg ccccggtcat tttatatgct ttacgtttac catttatatt
     5821 ttcatccata gggtataagt atatactgtc attacctgga tattgtttgg ctatttctac
     5881 aatattatta gttgtatcag tatcagacat actatctatt ttcaaataca attttttacc
     5941 actttctggc ttaatatttt ctacatttct agttcttcct gaattgtagt ttctatctgc
     6001 aaagtcagta tcatcattta tatcttttat ctctctagct ataagttttg cattttcgcc
     6061 ttcttttata cttaatgttc cttttacata tattatatta tcttcattca aaatgacatt
     6121 atacttttgt aataattgag gaaaaactat tatttctata gcaccataca aatcttctaa
     6181 ttcaataaat gccataattt catttcgttt tgttgtttta atagttttag ttacaatcat
     6241 tccacccatt ataacatctg tttcattcat ttgtaggtat gactcttctt cctcttttaa
     6301 tgaatttaat tttccattat ccatagatgt tttttcttgt agttcttttt caaattgtga
     6361 taaaggatgt ccacttacat acattcccaa gacttctttt tcaaggttaa gtctctctct
     6421 ttcttcgtac tcttgccttt taggcaaact gtatatttgt tgaaaatcac tatcttcctc
     6481 actatctcca aaagcatcaa ataaagaaat ttgaccttga acatttttct ttctatccat
     6541 agatacactt tctaataaac tttcataacc agccatcaga ctagctctat tttcacttat
     6601 attatcaaaa gctccacatt ttattagact ttctattact ctcttatttg tatctttttg
     6661 gtctaatctt ttgactaaat ctactaaatc tacaaataat ccattagatt ctctttcagc
     6721 gattatattg tttattatat ttacgccaac attttttacg gctgctagac caaatcttat
     6781 attgtcgcct tcaactgaaa actttgaaaa acttttgttt atatctggag gtaatacatc
     6841 tatttctagt gctttacatt ctcttatata ctcaactacc ttatcagtat ttcccattac
     6901 actggttatc aatgctgcca taaattcaac tggataatat gtttttagat aagctgtctc
     6961 atatgctaaa accccatatg cagctgcatg tgatttatta aatgcatatc tagcgaagtc
     7021 tatcatatca tcaaatattt tattagctat ttcctcttct acaccatttc tgatacaccc
     7081 tgcaatttct atattaccat cttcatcaaa tttaccatga ataaaatatt gtctttcttc
     7141 ttccatcacg tccatctttt tcttactcat ggctcttcta actaggtcac ttcgtccgta
     7201 actataacca cctaaatctc taacgacctg cataacctgc tcttgatata ccaaacaacc
     7261 atatgtaact tccattatag gctttaattt ttcatgtaga tatgttacat cttttgggtt
     7321 gcttttattt tttatatatg caggtataga atccattgga cctggtctaa atagggatat
     7381 tcccgctaca atatcttcaa aattgtctgg ttttagctgt ttcataaaac ttctcatgcc
     7441 agcactctcc aattgaaaca cccctagtgt atttcctgat gaaagcattt catatacttt
     7501 cgggtcgtca taatccatct ttgaaaagtc tattttttct gtgtaatttt ttataactct
     7561 atttttttct attaaatcaa gagtatctct tataactgta agagttctta gcccaagaaa
     7621 atccatcttt aaaagcccaa gttcttcaag tgttgtcata gtaaactgtg ttgtgattgc
     7681 atcttgatgt ttgtacaaag gtacatattc atctacggga ttttttgata taacaactcc
     7741 tgctgcatga gtagaagcat gtcttagcat tccctctatc tgttttgata tatttattat
     7801 ttcttttgtt tccgggtctt ggtcatacaa ttctttaaga ttaggatttg tatctagggc
     7861 cttatctatt gtcattccta aagcaaatgg tatttccttg gctaccttgt caactttatt
     7921 gtagcctata tccaataccc ttccaacatc tcttattgca gcttttgcac ccattgttcc
     7981 aaaagttatt atctgggcta catggtcatc accatatttt ctctttacat aatctataac
     8041 ttcttctctt ctctcataac aaaaatctat atctatatct ggcatggata tacgttctgg
     8101 atttaaaaaa cgctcaaata atagagagta ctttattggg tctatatcag ttattttaag
     8161 tgtatatgct actatcgaac cagccgcact acctctacct ggtcctacta taatattatt
     8221 ttctttagaa aaatttataa aatcccaaac aattaaaaaa tactctacat aacccatttt
     8281 ttcaattaca tctaattcat aatttaatct ctctagtatc tcattactag gattatcata
     8341 tctctctttt aatccattaa aacacaactc ccttaaataa gtttctggtg tatatccttc
     8401 tggaacatca tacttaggaa gatgtatagt attaaaatca aactccacat tacacatatc
     8461 agctattttc acagtattgt caatagcctc taatgcatat ggaaataatt cttccatttc
     8521 ttctcttgat tttaaataaa actcatcact accaaatctc attctatttg ggtcatttaa
     8581 tgtttttccc atttgaatac acataagtac atcatgtatt ttcgaatctt ctttatttac
     8641 atagtgaaca tcattagtag caactagtgg tataccagtc tcttttgata attttactaa
     8701 accagagttt acttcttttt gctcaggtaa attgtgatct tgaatctcca aaaagaaatt
     8761 ttcttctcca aatatgtctc tgtagtcaag agcaaattgt tttgcttttt cataatttct
     8821 attcattaaa gcacgtgcta catctcctgc aaggcatgca gaaagtgcta taatcccttt
     8881 gttatgtttt ttaagttctt ccatgtcaac tctaggctta tagtaaaaac cttctacgta
     8941 tgaagttgaa actattttta ttaaattttt atatccttcc atattcttag ctaacagaac
     9001 taaatgtcct tgatatttgt catagtttgg gtctttatct ctcaatccac gtgcagctgt
     9061 atagacttca caccctataa ttggctttat tccttctttt atagctgttt tgtagaaatc
     9121 tataacacca aacatacaac catggtcagt tatagctatt gagctcatgt ttagttcctt
     9181 agcccttttt ataagttttt tcaccctcga aaacccatca agtaaactat actcagtatg
     9241 tacatgaaga tgagcaaaat ctttgttcat taaatcacct cctgctttat aattttaatt
     9301 ttaccacaaa tatatgtcct aaaacaaaaa cagagacttt tatctcaaaa taggaattaa
     9361 cttcaacgta taccattata tgttataaat taatgcttat tctgagattg tcccctcaat
     9421 ttattgctat gatttttttt tcaaatctgt tgcaatttct tctatttttc taagtctatg
     9481 attgactcca gattttccta ttggtggttc taacatctct ccaagttctt tgagagtcat
     9541 atcctcatac tcgattctaa tcttagctat ctcttgtaaa ttttctggca aactgctaat
     9601 tcctatagtc tcttgtatga gcttaatatt ttctacttgt cttactgctg catttactgt
     9661 tttagaaagg ttagctgtct cacaatttac tatcctgttt acattatttc tcatttcttt
     9721 tactatttta gtattttgaa gacttaaaag agcgtggtgt cctcctataa tactcaacaa
     9781 atctgatatt tgttcactct ctttgatata aacaacgtga ttattttttc tagccacaat
     9841 tttactatta aaacctaaag agtttataag ttcttttaga gaatctgcaa actcctcatt
     9901 attagttaca aattccatat gatagttctt ttctgggtcg cttatagaac ctccaccaag
     9961 aaaagctcct cttatgaaag ctctcttaca tccttcatgc ttaattaagt tctctgaaat
    10021 tttgtttata gtaaaaaatc cttctttatc ttcaagtata cctaactttt ttaataacat
    10081 ttctgaaccc atttcgcttg ttaccattaa tacataactg ttatttctct ttagcatttg
    10141 attcttattt actgagattg tagtattgat attaaaattc ttttttaata atttaaatac
    10201 tttacgggct attgaattta actctgttgt tatctttaga tttagcttct tatatccaac
    10261 aatttgtata cttccccctg atttaactag agctgacaat tcagctatat tacaacattc
    10321 attcccagat ataactcttg ctagttcatt tttggtttca gttgaaaaag acatatctat
    10381 cacctattaa ctaatttttc tttaaccttt tttttatttc ttgtaaggca acctttatta
    10441 tctccttttc atcatcatac ctagctaagt ctactactct atctaggtgg ataaatttag
    10501 catcaataaa gccttcctct ttttgtggaa tagtatccat attctttgct tgcatcaaat
    10561 accaaaaaac tgttttatga actgctctat tttcatccca gttttctttg aatgtatagt
    10621 gtatttcacc taaatatttc aatatatctg cttttactcc agtttcttca cttacttcac
    10681 gtaatgctgc ctcttggtta ttttcccctt cttctacttt tcccttgggt aacacccaat
    10741 ctccattgaa cttccttagt aaaagaatcg tattgccaaa caggactaca ccaccagcac
    10801 taacctcttc tctcatcgat gttcactcct taaaactaac ataatgataa ggatttctac
    10861 acaatttttt aaaatccttt tttactaact tttgttgtaa ttatgattta gggctaactc
    10921 aataactatg ttagatatat actttgcatc atgtcttata taattatttt ttatttcaat
    10981 taaattcttc tctacagttt ttatacccat ctcttttaat ttatcttttt gctttttatc
    11041 taacaagact tgctctgctc catcttgttt atataagtca agcatttctt ctggcaatat
    11101 ctcattattt gcgattacat aatctattaa attctcatca gtgtgtttta ctatagcatt
    11161 aacatgctct aatacattgt acccttctgt ctcacctggt tgagtcatta tatttgctat
    11221 atatactttt ggagctacag acgatttaat agcgtctaca atcccctcta caagtaaatt
    11281 tggtattata cttgtatata aacttccagg acccataatt ataatgtcag catcataaat
    11341 agatgcaata acatccttta atggttttac atcttttgga tttaaaaaaa ttttatcaat
    11401 agagcttttt tgaatttttg actcttcagg tatactagat tctccattta ttatattgcc
    11461 attttctaat ttggcaacta aatttacatc ttccaaagtt actggtaaaa ctcttcctgt
    11521 tatagcaaaa atttcactta atttatatac agctttttca aagtttccat acaacccatt
    11581 catagcagcc aaaaacaaat ttccaaaact ctgacctttt aaaagccctt ctgtaaacct
    11641 atattgcata acttcattca ttgttggctc tatatttgca agtgctaata aacagtttct
    11701 tatgtctcct ggtgggagca ttcctaagtc ttctctcaat acacctgaac ctccaccatc
    11761 atcagcaaca gttacaattg cagttatatt ttgcgtagtg tgtttaagac ctcttaaaaa
    11821 taccgattga ccagtacctc cacctatcac aaccacattt ggttctctat tttcaacaat
    11881 agacctttga aatagtttta ctttttcatt ttttgctttt ttatatataa ataacgatac
    11941 aattaatgca atccaaccta aaattcctac aataataagt aatttagcca tgtttgcctc
    12001 cattatcgca acatagagtc tctatgtttc tttacaactc tatatccctt attagataaa
    12061 tcctccgcta taagatttgt tatagtaact gacctatgtc ttccacctgt gcatccaaca
    12121 ccaattacta gatgttgttt tccttcctct atatattgag gtactaaaaa atgaatcata
    12181 tctaaaagtt tcacataaaa ttcttcactg attttagaat tcataacata atctctaact
    12241 tctttatcat caccagtttt agctcttaac tcttcaacat aatagggatt tggaagaaat
    12301 cttacatcaa atactaaatc tgcatcagca agtataccat gtttaaatcc aaaagatact
    12361 acagaaatag ttaggtttgg attatcttca ccagaagaat atattttctt tatttcttcc
    12421 ttaaggtctt taggtttcat gttagttgta tcaattatac atgtagataa ctcctttaaa
    12481 ggttccatta tctttctttc catttttata ccttctggaa tctgcatatc ttttgccaat
    12541 ggatgatttc ttcttgtcat tttatatctc tttagcaata catcatcatt acaatccaaa
    12601 tataccattt catattcata attttctttt tctaaataat ttaagctttc atgaagcgct
    12661 tcaaaaaact ttctccctct tatatctatc cctagagcaa ctttatcaat acttgaattt
    12721 ggttgataac ataattctgc aaatttagtt atcaaagcag gaggtagatt atctacacaa
    12781 taaaatccca tatcctctaa agctctcatt gtctcacttt ttccagatcc tgaaagacct
    12841 gtaactataa caaatttcat tgctaactcc tcccttttat cttatgttta ttatattata
    12901 atgttttcta ctaacgatat atccagatta gagtcagata tagtcttcat agctagttca
    12961 aaatcaccaa ctctttctgg acaatgtgca tctgagccta ttataaattt attttctagc
    13021 ccttttattt tatttaattg atttacattc aaaaaagaat ggctactatt tatctcaagt
    13081 ctagtatttg tcttttttgc tgtctttgct acttcttcta tatacacatc gcctttatct
    13141 cctggatgag taattataaa aatatcatta ttacttatcg catttattac agcttttgta
    13201 ttatattctt ttgctttttc agttttaaaa atatagttat tacaatgatt taataagctt
    13261 cttaatgatg ttggagtaga accaaaatga tatccagcca taatataatc aaaatctcta
    13321 ataaatttat catccatatc tatatttcct ttatcatcta atatgttaca ctccatacct
    13381 aaaagtatat ttatatcagt gtacttatca tttaaataat ctacttcttc tctcattttg
    13441 agaatatctt ttttctttac accatatgca atatgtttat aactatggtc agatattcct
    13501 attgttttta ttcctttaga aatggctacc tttacattat cctcaatagt ccctttgcca
    13561 tgactatata atgtatgtgt atgataatca gccagtattt tcatttgtta atcctctcca
    13621 agtattttga cttcttcttc caacataacc ccaaacttgg catatacagc actttttata
    13681 acatatatta aatccaaaat atctttagca cttgcttctc cttgattgac tacaaatcca
    13741 caatgcttct cagatacttg tgctcctctt aaagtaagac ctctcaatcc agtttcttct
    13801 attaatttac ctgcaaaata tcctgttggt ctcttaaatg tacttcctgc actagcaaaa
    13861 tttaatggct gtttagttat tcttctcgtc gctaaatcat ccattaaact ttttatttca
    13921 tcataatttc cttcttgtaa ttctaattct gctgataaag ctatatatcc gtttttcgat
    13981 attatacttt ttctataccc aaattccatc tgttcatttg ataattcaaa tatattgcct
    14041 tccatatcca tcagtcttac tgacttcact atatccttca tttcaccacc atatgctcca
    14101 gcattcatgg ctaatgctcc tcccaatgtg ccaggtattc ctgatgcaaa ctcaaaccct
    14161 tttaattctt gcttttggag agctttccct aaaactgata ataaagctcc tgactgagca
    14221 gtcatcctag tatcatttat ctcataagaa ttgaaattat cagctaattc tattaccact
    14281 cctcttattc caccatcttt aattaaaatg tttgaaccat ttccaattat taaatacgga
    14341 atagactctt tttttaccaa cttaagtaca ttttttaatt gttcttcagt tctaggtcta
    14401 actaatatat ctgctggtcc tcctacccta aatgaaatat gttttttcat aggttcatcg
    14461 acttttatat cctctttact taatatattt aataaattta tatatatatc ttgattattc
    14521 attgtatttc ctcccaaatt atactgaact ttagctgtca ttattattta aattacatta
    14581 atatatagta ttaactatat tcaataatat atgattttgc ttatcatatg gattttatat
    14641 tttaagcttc ttaattacaa actatattca ataaatatat aataaagtaa aaaaaaaaaa
    14701 ataacaacat ataaaagaat ataaataatt taaaaaaagc atcaatatca ctgatatcaa
    14761 tgctttaaaa ggcaatttaa ttcgtattat ttatctaact atttttgaca tagcaaagat
    14821 aataaatccc tccaactact atggaacctc caacgatatt tccaagtgtt acaaatacca
    14881 aattagttat aaattgaacc atagttatat ttgctcctaa tgccattccc attggtataa
    14941 aaaacatgtt tgcaacacta tgttggaatc cacataatac aaatagcatt attactgtcc
    15001 aacatgaagc caattttgac attatatctt gagatgctgt agccatccat acagctaaaa
    15061 cgactagaat attacaaagt attgctttta aaaacattga tgctatagtt gaattaacct
    15121 tagcttctgc cacagctata gcttttgtag ctgcatctcc tgtcaatgta gaagaataaa
    15181 acacaagtgc aactagcaaa attgaaccta ctaaatttgc aaaataaacc agtccccagt
    15241 ttcttaacat ttttcctaat gttatttttt tgtccataag tgcaatagcc attaaagtgt
    15301 ttccagtgaa taattctgct cctgccacta ctacaagcat aagacctact ggaaagacca
    15361 tagcacttaa aaagctagct acccctgggt ctatcttgcc aaaagtttga ctaactatca
    15421 tactccccaa tgccccaaaa cctataaatg ctcctccaaa taaacctaat agaattgcat
    15481 tttgtgtcga aatatttgct tttttaatgc ctgcacttat agttgcttgt gcagtttccc
    15541 caggtgtcaa gaatccttta ctcataaatc cttccccctt aaaaattata ctttcccttg
    15601 tctttttatg gcaataatta tataataaca atatgatata taataaattt tattaacata
    15661 aaaaatatac tatatactta tatatacttg tctcatataa ttatactatt tattatataa
    15721 caactttgtt aatttcgaaa taatttatat tgaatttgaa aaatttacct tttgattatt
    15781 tattttact
//
LOCUS       DAYAPA010000101        15653 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.101, whole genome shotgun sequence.
ACCESSION   DAYAPA010000101 DAYAPA010000000
VERSION     DAYAPA010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15653
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.101"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(21..251)
                     /gene="dltC"
                     /locus_tag="ACGI59_002840"
     CDS             complement(21..251)
                     /gene="dltC"
                     /locus_tag="ACGI59_002840"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036370 - D-alanyl carrier activity
                     [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HFG9740619.1"
                     /translation="MQETVIEIFEDVLGTDEIREDLDLNLFETELLDSLAIIEVLLEI
                     ENRLGIELQPTDLERKDMSTVNNLVKFLETRK"
     gene            complement(270..1418)
                     /gene="dltB"
                     /locus_tag="ACGI59_002841"
     CDS             complement(270..1418)
                     /gene="dltB"
                     /locus_tag="ACGI59_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HFG9740620.1"
                     /translation="MIFSQYGDYFYLYILLLTSIPAVILGLMGKNIKYYGMLASLFMI
                     FLIVGIDVQLKYLVIFIILEVIIVKGYEYVRRKTKNKYIYWGFLFASMLPIIINKISP
                     VTSFGIIGFIGISYLNFRTIQMVIEIYDGAIKEVKISKMLYFMLFFPTLSSGPIDRSR
                     RFEQDLEKQISRKEYIEEYLLPGIKNIVMGVGYKFVIAFLINTYWVSRIPKDMSFINI
                     LSYMYAYSLYLFFDFAGYSLFAIGTGYIFGIQVPINFDKPFISKDMKEFWTRWHISLS
                     RWFGDYIFSRFVMSSMRKKRFKKRTTAAHVAQMITMITMGFWHGLTWYYVAYGVYQGL
                     ALVLTDIYQRKSKFYKKHKKDKWFERVQIFITFHIVCFGLLIFSGYLA"
     gene            complement(1418..2932)
                     /locus_tag="ACGI59_002842"
     CDS             complement(1418..2932)
                     /locus_tag="ACGI59_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase"
                     /protein_id="HFG9740621.1"
                     /translation="MKIIEGIKKYSNTDRTALMCNGDKLSYKDLNEYSDAISVFLKDV
                     YKEEDTPIVIYGNKENMIMACMIGALKSGRAYVPLDISFPIDRVFEVTKEIKPKVLFN
                     FSDEKDFGDINVIDMDKLNYIINEYQGKSLDKENWVKDDENAYILFTSGSTGKPKGVQ
                     ISSNNLDSFSDWISPYLNIDGSEKVIMNQPAYSFDLSVTTIYPGLIHGATLFSISKDV
                     LADYKELFRQFSISDIAVWVSTPSFAGVCITEKEFNSKMLPNLESMIFIGEALSKNLT
                     KELMSRFPNTRIINGYGPTEATVGVSVNDMTQKAIDDEKSLPVGYPMSNCKIKILDED
                     GNELKENEKGEIIIIGPSVSKGYFNNKEKTDEVFFYDEIDGVKWRAYKTGDMGYLLDG
                     NIYYCGRKDFQIKLNGFRIEIEDIENNLRKVHNVKNAVVLPVYKDEKIAYLKGIVELN
                     EKNDLSNIKNGMIIKKELGKYIPSYMIPRNISIISEFPTNINGKIDRKKLMEEI"
     gene            complement(3053..4243)
                     /gene="dltD"
                     /locus_tag="ACGI59_002843"
     CDS             complement(3053..4243)
                     /gene="dltD"
                     /locus_tag="ACGI59_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070394 - lipoteichoic acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HFG9740622.1"
                     /translation="MRKLIYFITPFIIGVVFLFGLDKFLDSKTDELLREKNLLPIMDD
                     TLSDIKDKGVTANNHFLREKDIMILGSSELSNSTKQHPKYYFNTNRSKNKVFAIGRAY
                     TQTLQDAAILGSMNPNIDNKKVVLLISMQWFMEKDGVTSHHYQSRFSPIQFYRFLDNP
                     KISKQNKIEYAKKSSKLLWGSDEYKAEALYAKLYEPKTFPEKAEKVLLEPYFQGRKYC
                     IALKEKGILYKRLIKLDKKRATKRKRPINWSYERKKAIEDAKKRVGKNPLNIDKYYYK
                     EHFKDGIEQYKGRDKDVNLLTSKEFENYKLMLNVCTDLGIKPVVVLIPSMDKFYNLTG
                     ISEKERNQYYDKAQKIAESKGFEVLNLKDKGSDKYYLRDVMHLGTKGWVDVCERLFKI
                     FKEQ"
     gene            complement(4531..5550)
                     /locus_tag="ACGI59_002844"
     CDS             complement(4531..5550)
                     /locus_tag="ACGI59_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFG9740623.1"
                     /translation="MMGKIRFGIVGTSNIANIFLRAASRVKDFELVAVYSRNLEKARE
                     FGTLYGASIFFDDLEQMAKSKDIDAVYIASPNALHSKQTITCLKHKKHVLCEKSLASN
                     LKEVKSMIKTAKDNNVLLMEAMRITCVPNFKAVKENLYKIGKIRRFFGSYCQYSSRYD
                     KYKNGIIENAFKKELSNGALMDIGVYCIHPMVNLFGAPKTVKAVSHILQTGVDGEGSA
                     VFQYDDMDAIIQYSKVADSYIPSEIQGEEGSIIIEKLNLFEKAIIKYRDGREEDISIP
                     KEKAEEKPREIEGMYYELVEFISLINNNKIESNINSHENSIIVMEIMDEIRRQSSIVY
                     PADSI"
     gene            complement(5726..6265)
                     /locus_tag="ACGI59_002845"
     CDS             complement(5726..6265)
                     /locus_tag="ACGI59_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease"
                     /protein_id="HFG9740624.1"
                     /translation="MDNVGSLLYFLFNFFLYGFIGWIIENLYSYYKQGHFQEDGFLNG
                     PFKPMYAIAMSIIIEVSRFIDPNNIFALLLLCLVVPTVVEYITGILMKVYFNKVYWDY
                     SDYKYNSRGIVCLKFSVYWTILTFIGVRYFQVYIVDSLYSLIIPYWPIVVTILLVTLF
                     VDEILTIKFLRGNKDIVER"
     gene            complement(6366..7046)
                     /locus_tag="ACGI59_002846"
     CDS             complement(6366..7046)
                     /locus_tag="ACGI59_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFG9740625.1"
                     /translation="MQKVEGIIFDMDGVLFDSERISLEFWMETFEKYGYTMTKEIYTS
                     VMGRNRKGIIEGLTDIYDSSVPIIDLYDEKTKNMIEFMERKGAPIKLGVNELISFLKE
                     NGYKMAVATSTKRERAVKRLAKANLKDYFDAIVCGDDVVNSKPNPEIFLKAAKKINVN
                     PQNCIVIEDSPMGVEAAYNGGIRCINVPDLKEPDEQIKSQSHKILENLLEVREYLKSL
                     NSKECHNL"
     gene            complement(7075..8364)
                     /locus_tag="ACGI59_002847"
     CDS             complement(7075..8364)
                     /locus_tag="ACGI59_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFG9740626.1"
                     /translation="MRELLKVTLIVMLVLINAFFVACEFAMVKLRSSRIDTMITEGNN
                     NAKRCKIIKDNLNSYLSACQLGITLCSLALGWMGESTVKELILPIVSVFNLSESIIYT
                     ISISISFLIITMMEVVIGELVPKALALYNTEKIMLSTSFLLVGFYKLTYPIIYFFNLS
                     TDLFLKPFGYSQADEVDEPHTGDEIRLLVEESYKSGLIDESEQRLVDNIFEFEEKKIR
                     EIMVPRTDMVCIYESDSEEKILAILKEEGVTRYPVCRKNKDDILGFVHIRDLYNQKIN
                     ENKIELEEILRDIIYISENLTIDKALERIRKEKLQLAIVVDEYGGTSGVVTIEDILEE
                     IVGEIQDEFDKEETHIKKIGAGSYLVDGTESINYINKYFGLDIEHDGFDSIGGWISYM
                     LGSNIKVNQSVTFENYNFTILELDKLRVVKLIIKSVI"
     gene            complement(8813..10414)
                     /locus_tag="ACGI59_002848"
     CDS             complement(8813..10414)
                     /locus_tag="ACGI59_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFG9740627.1"
                     /translation="MIYDLILKNGFIIDGTGNPGFYGDIAIKDNLIAKIDSKINSNTN
                     KEIDCYGKIITPGFIDPHVHEEIVAILDGKFEKFLKQGVTTTINGNCGHSITPYSSEN
                     VYEYMYKNGLLLEEEKKYLIDKNKCWNNFTEYCDLISKSGISVNMGFLLGHGTIRWSV
                     MGGSKDRPPTEKEKNEITDIINDGMKSGAFGISTGLAYIPSKYADIDELVDIARQIKE
                     HDGIYTSHIRDYIGRYNAVKEAIEVGQKSGARVQVSHLSPVEIEAFDEILKARYNGVE
                     IMVDTVPRSSGHCMKKKRVIQFIMAISSSLFELGIDGVMDALRNEEGRTLILKEAFIL
                     GDRGSIILLNTKDINMEKKSIREIATQKGIDEDKLLLDLLLDGDEELIFCLGGMYRAD
                     FPDKLHDDKIIDNPFVMVGSDCLFSVGGDMSWFELQRRGAFPIFFNMYRKSGVRLEEI
                     VRRVTSLPARQFKIKNRGILKEGLIADIAVIDMDNYSYPRSEDIDFSKPQLLANGVEY
                     VIVNGKIALENGEITENKCGEVLKR"
     gene            complement(10525..11202)
                     /locus_tag="ACGI59_002849"
     CDS             complement(10525..11202)
                     /locus_tag="ACGI59_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9740628.1"
                     /translation="MSKVKSIYNEEYLPFMIRYGRLTLSLGIIAALVPGIILSFGFGI
                     MPPISALLASTMAIVSMSAPNYIIEPVSYSPILGIPGTYMSFLSGNISNMRLPCSIAA
                     QKAAEVESGTEEGSIISTIGIAVSILVNISILTIGVILGGSVLSKIPAEVVEKLNLIL
                     PALFGSVFGQVFLQDKKLGLVAIVISVLTIILSKQGIIPQSLVVLICVFGTILIARAM
                     YKDKLSD"
     gene            complement(11215..11910)
                     /locus_tag="ACGI59_002850"
     CDS             complement(11215..11910)
                     /locus_tag="ACGI59_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5058 family protein"
                     /protein_id="HFG9740629.1"
                     /translation="MDYLKLANHPLLWIASTVAVSIVVVQSLIFAIKSCKVGKTMGIT
                     DKQIKSAVKSSAISAIGPSMTIFAGMVSLIVTMGGPIAWMRLSFIGSVIFESMSAGFG
                     TDALGITLGSPEMTKLAFTNAVWTMILGSLGWIIFTLLFGHKLDKITNVISSGKKSFI
                     PIISLGAMLGSFAYLNADRVLRFDNGTIACISGMAIMVILCMLEKKKNIKWLREWGLT
                     ISMFSGMIIASVI"
     gene            complement(11962..13161)
                     /locus_tag="ACGI59_002851"
     CDS             complement(11962..13161)
                     /locus_tag="ACGI59_002851"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HFG9740630.1"
                     /translation="MKSAYKGYEAYSYLEKGKDISSIEMCKGDKRVKEYLIELDDIQE
                     AKVKSIVDEKIFISLHEHPVLFPEHLERDIFEYNREGKQRCAYEALSRGYYDAIFDNL
                     MDGVCTITSNSGWKWDDILIDLGMRLCDLAHQDFVIKCEKVEDIYRAKSEGKVALIPT
                     LEGAAPIENELDRIDILYGFGIRLMGITYSESNALGSGLKEEKDGGLTNFGKKAVERM
                     NKIGMAIDCSHVGVQTTLDVIEYSKDPIILSHVGARSLWNTKRLTPDEVLIACAKKGG
                     VIGVEAAPHTTITEKNKEHSIESFMEHFEYIKNLVGIDHVAFGPDTVYGDHVKLHSVL
                     AAKFDIKKITGTKEHPKVEYVKGLENPTETSKNIVRYLVKHGYSDEEIEKVLGGNILR
                     VLKQVWK"
     gene            complement(13422..15467)
                     /locus_tag="ACGI59_002852"
     CDS             complement(13422..15467)
                     /locus_tag="ACGI59_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFG9740631.1"
                     /translation="MKKHNILFVSTDDKINIDISKQLENIFGEFCNIDNLVYVNRINI
                     ELSSYELVVCSDNDIKEYIHNNIDKNIPIVIVHRTINIENINQIISIENDSDVMVIDA
                     YKESADETAKIIRKLGLIHINLIPYYPGCDKSKCEIGIITGSRNSIPQNIKQIIDIGD
                     KVIDINTVIEIFTKLNISIDKLHIIKEKYNEDTVSGYRYYTTMNKTMKSFLEIIDEGI
                     ASVDKLGKFIYCNKVFSNLVGIDQNEIISNNFMDLFSDKVVKKIFFQEDEVNDEVVNL
                     NNKKLIINKVNVYENNERIKSIISIKNISAIQVLEDKIQNKLQSKGFVSKYTFESVVG
                     ESKIIKEKINIARKIATTDFSVLILGENGTGKEIFAQAIHNESLRKNKPFVAVNLSSL
                     SDTLIESELFGYDEGSFTGAIKGGKMGIFERAHTGTIFLDEIGDISLDVQQRLLRVLQ
                     EKEVMRMGGSKIIPIDVRIIAATNKDLKKKIKEGSFREDLYYRINVLHIEIPRLRERK
                     EDIPLISKYFLDEINSNKCFTEESMQALTLYEWPGNVRELKNLIYYIDTIVEEDRVDY
                     EHLPEQFRFEKNNTLVNENFDSIILDFKQSNFFEESICILTSIETWNNKNILLGRNKL
                     QEILKEKDIVLSVDQIRKRIDKLKSHGLLLSGVKKQGSFITDEGKNFISYVKIKGVI"
BASE COUNT         5045 a         2838 c         1526 g         6244 t
ORIGIN      
        1 tttaaagtct aactactaat ttattttctt gtctccaaga attttactaa attatttaca
       61 gtagacatat cttttctctc taaatcagtt ggttgaagct ctatccctaa tctattttca
      121 atttcaagta aaacttcaat tatagctaat gagtctaaaa gttccgtctc aaataaatta
      181 aggtctaaat cttctcttat ctcatcagtc cctaatacat cttcaaatat ttctataact
      241 gtttcttgca taaattttcc tccttaaaat taagctaaat aacctgagaa tatcagcaat
      301 ccaaaacaca caatatggaa tgtaatgaaa atctgcactc tctcgaacca tttatctttt
      361 ttatgttttt tataaaattt agattttcgc tgatatatat cagttaacac aagtgccaat
      421 ccttggtata caccataagc aacataatac caagttagac catgccaaaa tcccattgtt
      481 atcatggtta tcatctgagc cacatgagca gcagtagtcc ttttcttaaa tcttttcttt
      541 ctcattgatg acataacaaa tctagaaaaa atataatctc caaaccatct tgaaagactt
      601 atatgccatc ttgtccagaa ctctttcata tctttactga taaatggctt gtcaaagttt
      661 ataggtactt gtattccaaa tatgtatcct gtacctatag caaataaact atatcctgca
      721 aaatcaaaga acaggtataa actatatgcg tacatataac ttaaaatatt tataaaactc
      781 atatctttag gtattcttga tacccaatat gtatttatca aaaatgctat aacaaactta
      841 taacctactc ccatgacaat attttttatt cctggcagta agtattcttc tatatactct
      901 tttctagaga tttgtttttc caagtcctgt tcaaatcttc ttgacctatc aataggtcca
      961 gaacttaaag ttggaaaaaa taacataaaa taaagcatct ttgaaatttt aacttctttt
     1021 atagctccat cataaatttc aataaccatt tgtatagttc tgaaatttaa gtatgatatt
     1081 ccaataaatc caattattcc aaaagaagta actggagata ttttatttat tattattggg
     1141 agcattgatg caaacaagaa tccccaatat atgtatttat ttttagtttt tcttcttaca
     1201 tattcatatc ctttaactat tattacttca agaataataa atattaccaa gtactttagt
     1261 tggacatcaa ttcctactat taagaatatc ataaacaaac ttgccaacat accataatac
     1321 tttatattct tgcccattag acctaaaatt actgctggta ttgatgttaa taataatata
     1381 taaagataaa aataatctcc atattgtgaa aaaatcatta aatttcctcc ataagtttct
     1441 ttctgtctat tttaccattg atatttgttg gaaactcaga gattattgaa atatttcttg
     1501 gaatcatata tgaaggtata tactttccaa gttccttttt tattatcatc ccatttttaa
     1561 tattacttaa atcattcttt tcatttaact caacaatacc ttttaagtaa gcaatttttt
     1621 cgtccttata tactggtaaa actactgcat tttttacatt atgtactttt ctcaaattat
     1681 tttctatatc ttctatttca attctaaatc catttaattt aatctgaaaa tcttttcttc
     1741 cacaataata tatatttcca tctaaaagat atcccatatc tccagtttta taagctctcc
     1801 actttacacc atctatttca tcatagaaaa atacttcatc agtcttttct ttattattga
     1861 agtaaccttt agaaactgat ggtccaatta ttataatttc acccttttca ttttctttaa
     1921 gctcatttcc atcttcatca agtattttta ttttacaatt tgacataggg tatccaactg
     1981 gaagactttt ttcatcatct atagcctttt gagtcatatc atttacactt acaccaacag
     2041 tagcctcggt tggtccatat ccattgataa ttcttgtgtt tggaaatcta ctcataagtt
     2101 ctttagtaag attttttgaa agtgcttctc ctatgaatat cattgactca aggttaggaa
     2161 gcattttact attaaactct ttttcagtta tacatactcc tgcaaaagaa ggagttgaca
     2221 cccatacagc tatatctgag atactaaatt gtctaaatag ttctttatag tctgctaata
     2281 catcttttga tattgaaaat aatgtagctc catggataag accagggtat attgttgtaa
     2341 ctgaaaggtc aaaagaatat gctggttggt tcattattac tttttcactt ccatctatgt
     2401 ttagatatgg gcttatccaa tctgaaaagc tatctaaatt gtttgaactt atttgaaccc
     2461 cttttggctt tcctgtactt cctgaagtaa atagtatata tgcattttca tcatctttta
     2521 cccagttttc tttatctaat gattttccct gatattcatt tattatataa tttaatttat
     2581 ccatatcaat tacattgata tctccaaaat ctttttcatc actaaagtta aataatacct
     2641 ttggttttat ctctttagtg acttcaaaaa ctctatcaat tggaaaacta atatcaagag
     2701 gcacatacgc tctacctgat tttaaagctc ctatcataca tgccattatc atgttttctt
     2761 tatttccata aattactata ggagtatctt cttctttgta tacatccttt agaaatactg
     2821 atatagcatc tgaatattca tttaaatctt tataagaaag tttatctcca ttacacatta
     2881 gtgctgttct atctgtattt gaatattttt ttattccttc aataattttc atataagaat
     2941 gctcctttaa aatccgttat aaataaatgg caattcttta aatggagtaa atgttagaac
     3001 tcctatcgcc aatattataa ccatagttaa acaaatacta gttaacaaat ctttattgtt
     3061 ctttaaatat tttaaataat ctctcacaga catctaccca tcctttcgta ccaagatgca
     3121 ttacatccct taaataatac ttatctgacc ctttatcttt aaggtttaga acttcaaagc
     3181 ctttgctttc tgctattttt tgtgctttat cgtagtactg atttctttct ttttcagaaa
     3241 taccagtcaa attatagaat ttatccatac ttgggattaa tacaacaact ggcttaatac
     3301 ctaagtcagt acatacattt agcatcagtt tataattttc aaactcctta gatgttaata
     3361 aatttacatc tttgtctctt cctttgtatt gctctatacc atctttaaaa tgctctttat
     3421 aatagtactt atcaatattt agtggatttt taccaactct ttttttagca tcttcaatgg
     3481 ctttctttct ctcatatgac caatttattg gtctttttct ttttgttgct ctctttttat
     3541 ctagcttaat taatcgttta taaagaatac ccttttcttt aagagctata caatactttc
     3601 ttccttgaaa ataaggctct aaaagcacct tttcagcttt ttcaggaaat gtcttaggct
     3661 catatagctt agcatataat gcttctgctt tatactcgtc acttccccat aataacttac
     3721 ttgatttttt tgcatattca atcttatttt gtttagatat ttttggatta tctaaaaatc
     3781 gataaaattg tattggtgaa aatctagact gataatggtg acttgtgact ccgtcttttt
     3841 ccataaacca ttgcatagaa attaacaaga caactttctt attatcaata ttaggattca
     3901 tacttcctaa tattgcagca tcttgtaatg tctgagtata ggctctccca attgcaaaca
     3961 ccttattttt actcctattt gtattaaaat agtactttgg atgttgtttt gttgaattac
     4021 taagttctga tgaccccaaa atcataatat ccttttctct taaaaaatga ttatttgctg
     4081 taaccccttt atcttttata tcacttaaag tatcatccat aattggaagt agatttttct
     4141 ccctcaaaag ttcatcagtt ttagaatcaa gaaatttgtc taacccaaat aaaaatacta
     4201 cgcctattat aaaaggtgta ataaagtata tcaattttct catattttct ctcctctaaa
     4261 aatattcaaa tttatgttat tattaatcat attagttttg atatgtatat tcaactacaa
     4321 aattgtaata aaatcgtcaa aacttttttt gatactgtta caaaattgta aaaaaaaaga
     4381 aaaaaacgcc aaaaataaat ttctttttag cactttttca tacttataat ttgacatttt
     4441 aacacttggt taagtataca ccttacctta acttaaaatt aaatttattt aagaatatat
     4501 attataaatt cttatatttt ctaatataaa ctatatacta tcagctggat aaacaatact
     4561 actttgtctt cttatctcat ccataatttc cattactatt attgaatttt catgagaatt
     4621 tatatttgat tctattttat tgttatttat aagacttata aactctacaa gttcataata
     4681 cattccttct atttctctag gcttttcctc tgctttttct tttggaatag atatatcttc
     4741 ttctcttcca tctctgtatt taataatagc tttttcaaat agattaagct tttcaattat
     4801 tatacttccc tcttctcctt gtatttcaga tggtatataa gaatcagcta ctttagaata
     4861 ctgaattatc gcatccatat catcatattg aaatacagct gaaccttctc catccactcc
     4921 agtttgaagt atatgagaaa ctgcctttac agtttttgga gcaccaaata aattcaccat
     4981 aggatgtata caatacactc ctatatccat taaagctcca tttgacaact cttttttaaa
     5041 ggcattttct ataattccat ttttatattt atcatatctt gaagaatact gacaatagct
     5101 tccaaaaaat cttcttattt tccctatttt atataagttt tctttaactg ctttaaagtt
     5161 tggaacacat gtaattctca tagcttccat aagcaataca ttattatctt tggctgtttt
     5221 tatcattgat ttgacttctt ttaaattaga tgctaacgac ttttcacaaa gcacatgttt
     5281 tttgtgtttt aaacaagtaa tggtttgctt tgaatgtaat gcatttggag aagctatgta
     5341 tacagcatct atatccttcg atttagccat ttgttctaaa tcatcaaaaa aaatacttgc
     5401 tccatataga gttccaaatt ctctagcttt ttctaaattt ctagaatata cagcaaccaa
     5461 ttcaaagtct ttaaccctag atgcagctct tagaaatata tttgctatat tgcttgtccc
     5521 aacaatacca aatcttattt tacccatcat aaatcactcc ccattttcta cactttattt
     5581 taattttaat aaaaaacttt acaacttata gatatattat aaaataattt tatcaaataa
     5641 aaaagaaagc taattttaaa tgatttcacc attttaacat agctttcttt taactacaat
     5701 ctaaattcaa cttatatttt taaagttatc tttctactat atctttattt cccctcaaaa
     5761 attttatagt aagaatttca tccacaaaca atgttactaa aagtatagtt acaacaattg
     5821 gccaataagg tattataaga ctatataaac tatcaactat atacacttga aaatatctaa
     5881 ctccaataaa tgtcaatata gtccaatata cagaaaactt tagacatact attcctctac
     5941 tattgtactt ataatctgaa taatcccaat agaccttatt aaaatatact ttcataagta
     6001 ttcctgtaat atattctact actgtcggca ctactagaca taataaaagc aatgcaaata
     6061 tattattggg gtcaataaat cttgagacct ctattataat cgacattgca attgcataca
     6121 ttggtttaaa tggaccattt aaaaatccat cttcttgaaa atgcccttgt ttataataac
     6181 tatataagtt ttcaattatc caacctataa aaccatacaa gaaaaaatta aataaaaaat
     6241 ataatagaga acctacatta tccatgaata ttcctcctag ctgtttatac ttattttttc
     6301 tcatattcat ggatttattc taaatcaaag aaattatatt taatttattt aattaatact
     6361 ttatattata gattgtgaca ttctttagag ttcaaacttt ttaggtattc tctaacttcc
     6421 agtaaatttt caagtatctt atgtgattgt gatttaattt gctcatctgg ctcttttaaa
     6481 tctggtacat ttatacatct tataccacca ttgtatgcag cttcaactcc cataggcgaa
     6541 tcctctatga caatacaatt ttggggatta acatttatct ttttagctgc ttttaagaat
     6601 atctcagggt ttggtttaga gtttacaaca tcatctccac aaactatagc atcaaagtaa
     6661 tcttttaaat ttgccttagc taatctcttt actgctcttt ctctctttgt tgatgttgca
     6721 acagccattt tgtatccatt ttcctttaaa aaagagatta gttcattgac tcctaatttg
     6781 ataggagctc ccttcctctc cataaattca atcatatttt tagttttttc gtcatataaa
     6841 tctattattg gtacagaact atcgtatata tctgtaagac cttctattat tcctttacga
     6901 tttcttccca taactgatgt ataaatttcc ttagtcatag tatatccata cttttcaaac
     6961 gtctccatcc aaaattcaag agaaattctc tctgagtcaa acaaaactcc atccatgtca
     7021 aaaataattc cttctacttt ttgcatacaa ttcctccaat attttttgat ataattatat
     7081 tacacttttt atgattaatt ttacaaccct caatttatcc aattctaata ttgtaaaatt
     7141 gtaattttca aaagttacac tttggtttac tttaatattt gaaccaagca tataggatat
     7201 ccacccacct atactgtcaa atccatcatg ctctatatca agtccaaaat acttattaat
     7261 atagttgata gattcagttc catcaactaa gtaagaacct gctcctattt tcttaatatg
     7321 agtctcttct ttgtcaaatt catcttgaat ttctccaact atctcttcta gtatatcttc
     7381 tattgttacc acaccagatg taccaccata ttcatccaca actatagcaa gttgaagttt
     7441 ttcctttctt attctctcaa gagctttatc tatagttaaa ttttcagata tatatataat
     7501 atctctcaat atttcttcta gttcaatttt attttcattt atcttttgat tgtataaatc
     7561 tcttatatgt acaaaaccta atatatcatc tttatttttt ctgcatactg gatatcttgt
     7621 aacaccttcc tcttttaaaa tagctaatat cttttcttca gaatcacttt catatataca
     7681 taccatatca gttctaggaa ccattatctc tctaattttc ttttcttcaa attcaaaaat
     7741 attatcaact aatctttgct ctgattcatc tataagtcca cttttataac tttcttctac
     7801 taatagtctt atttcgtcac ctgtatgggg ttcatcaacc tcatcagctt gtgaatatcc
     7861 aaatggttta agaaataaat cagtacttaa gttgaaaaaa tatattattg gatatgttaa
     7921 cttataaaat cctactaata aaaaagatgt actcaacatt attttttctg tattataaag
     7981 tgctaaagct ttaggaacca actctcctat tacaacctcc atcattgtta taattaaaaa
     8041 agaaatggaa attgatatag tatatataat actttcacta agattaaata cacttactat
     8101 tggtaaaatt aactctttaa ctgtagattc tcccatccaa cctaatgcaa gtgaacaaag
     8161 tgttatacca agttgacatg cagaaagata ggaatttaag ttgtctttta ttattttaca
     8221 tctttttgca ttattattac cttcagttat catggtatca atcctagaac tacgaagttt
     8281 taccatagca aattcacaag ctacaaaaaa agcatttatc aaaacaagca ttactataag
     8341 tgttactttt aataactccc gcataaagta ttggttctta taatgtaaga aatctataag
     8401 aactcaaagt tcccccctta tattaataaa tttaatttat aatatatatt ctatcatatt
     8461 tattaatata tcctacttta ttacaaataa tttttataaa ctaaataacc ccttttaata
     8521 tttatgactg gctatttgat aaatattaaa ggggttattt agttatcatt ttttatttct
     8581 aaaaatcttt tctcaaatga tttagttatc tatttatcca attttttaca taatttaatg
     8641 attaaaaata ttaatttttt cactataaag tataaatatt atctgattat taaaataaat
     8701 attaatacta taaataaaaa cgaagcatga tttttattta aataaatctc aaacttcgtc
     8761 tttacttact tagatattta tttactttct aaatcctatt atttttaaaa ccttatcttt
     8821 ttaatacttc accacattta ttctcagtaa tctctccatt ttctagcgca atttttccat
     8881 ttacaattac atattcaaca ccattggcta gtaattgtgg ctttgaaaaa tcaatatctt
     8941 ctgacctagg ataactatag ttatccatgt caataactgc tatatcagct atcaaccctt
     9001 ctttaagaat acctctattt tttattttaa attgcctagc tggtaatgaa gtaactcttc
     9061 ttacaatctc ttccaatcta acaccagact ttctgtacat attgaagaaa attggaaaag
     9121 ctcctcttct ttgtagttca aaccatgaca tatccccacc cacactaaac aaacaatctg
     9181 aaccaaccat aacaaatgga ttatcaatta ttttatcatc atgtagttta tctgggaaat
     9241 cagctctata cattcctcct aaacaaaaga taagttcttc gtctccatct aaaagtaaat
     9301 cgagtaataa cttgtcctca tctattcctt tttgtgtagc tatttcacgt attgatttct
     9361 tttccatatt aatatcttta gtgtttagaa gtattatact ccccctatct ccaagtataa
     9421 atgcttcttt taatataagt gttcttccct cttcatttct aagagcatcc attaccccat
     9481 ctatacctaa ttcaaataga ctagatgata ttgccataat aaattgaatt actctttttt
     9541 tcttcataca atgcccagaa cttcttggta ctgtgtcaac cattatttca acaccattat
     9601 acctagcctt taatatctca tcaaatgctt ctatttcaac tggacttaag tgagatactt
     9661 gaaccctagc accacttttc tgcccaacct ctatggcttc ttttactgca ttatatcttc
     9721 ctatgtaatc tctaatatga gatgtatata taccatcatg ctctttaatc tgccttgcaa
     9781 tatcaacaag ttcatcaata tcagcatact tacttggtat atatgccaat ccagtagata
     9841 ttccaaaagc accacttttc attccatcat ttattatatc tgtaatttca tttttttcct
     9901 tttctgtagg aggtctatcc ttagaacctc ccataacact ccatcttata gtcccatgcc
     9961 ccaacaagaa tcccatattg acagaaatac cacttttaga tataaggtca caatattctg
    10021 taaaattatt ccaacattta tttttatcta taagatactt tttttcttct tcaagtaata
    10081 gaccattttt atacatatat tcatatacat tttcagaaga atatggtgtt atagaatgtc
    10141 cacaattacc atttatagtt gttgtaactc cttgttttag aaacttttca aatttaccat
    10201 ctagtattgc tactatttct tcatgaacat gagggtcaat aaatccaggt gttataattt
    10261 ttccataaca atcaatctct ttatttgtat tgctatttat cttagaatct atttttgcta
    10321 ttaagttgtc tttaattgct atatctccat aaaaaccagg attacctgta ccatctatta
    10381 taaatccatt ttttaagatt aaatcatata tcaaaatatt ccctccttaa aaaccaattt
    10441 ttaaataatc ttaatgtaat ttttatattt gaaaatcaaa ttattaaatt tcatatttga
    10501 aaattcacta ttatttcatt gtaattagtc actaagctta tctttataca tagctcttgc
    10561 aattaatata gtaccaaata cacatataag aactactaga gactgtggta ttataccttg
    10621 ctttgataat ataatagtca atacagatat tacaattgct actagaccta attttttatc
    10681 ttgtaaaaat acttgtccaa aaactgaccc aaatagagct ggtaaaatta agtttaattt
    10741 ttctactact tcagctggaa ttttagatag tacgcttcct cctaaaatta cccctatagt
    10801 tagtatactt atattcacta aaatagatac agctattcct attgtagata ttatcgagcc
    10861 ttcttctgta cctgattcaa cttctgctgc cttttgagct gctattgaac atggtagcct
    10921 catattagat atattaccag aaagaaaact catataagtt cctgggatac ctaagattgg
    10981 tgaataagaa actggttcta ttatgtaatt tggagcactc atactgacaa ttgccattgt
    11041 acttgctaat aatgctgata ttggaggcat aataccaaat ccaaagctca aaattattcc
    11101 aggaactaat gctgctataa tacccaaaga tagtgttagt cttccatacc ttatcataaa
    11161 aggtaaatat tcttcattgt atatagattt tactttactc ataaaactcc ccccttatat
    11221 tactgaagct attatcattc cgctaaacat tgatattgta agcccccact ctcttaacca
    11281 ttttatattt ttcttctttt caagcataca caatattacc attatagcca ttccacttat
    11341 acatgcaatt gttccattat caaaacgcaa caccctatca gcatttagat atgcaaatga
    11401 acccaacatt gctcctagag atattattgg tataaatgat ttcttgccac tagatataac
    11461 attggttatc ttatctagtt tatgtccaaa tagtaatgtg aatattatcc atcctaaaga
    11521 acctaatatc atagtccaaa ctgcatttgt aaatgcaagt tttgtcattt caggagaacc
    11581 taaagttata cctaatgcat ctgttccaaa tccggctgac attgattcaa aaattacaga
    11641 acctataaat gataatctca tccatgctat aggcccaccc atagttacta taagcgatac
    11701 cattccagca aatatagtca ttgaaggccc aatagctgaa atagcactac tcttaacagc
    11761 tgactttatt tgtttatctg ttattcccat tgttttacca actttgcaag attttatagc
    11821 aaatataagg gattgtacca ctactattga tactgctact gtactcgcta tccacaaaag
    11881 tggatgatta gctaatttta aataatccat ttataaaacc tccctataaa tcctcaaatt
    11941 ttattaatat ttctaatata tctatttcca aacctgtttt agtactctaa gtatatttcc
    12001 acctagtacc ttctctattt cctcatcact atatccatgc tttactaaat atctaacaat
    12061 gttttttgaa gtttctgttg ggttctcaag accttttaca tattctactt tgggatgttc
    12121 ttttgttcct gttatttttt ttatatcaaa tttagcagct agtactgagt gtaattttac
    12181 atggtcgcca tatactgtat ctggtccaaa agctacatgg tctattccta ctaaattttt
    12241 aatatactca aaatgttcca taaatgactc tattgaatgt tctttattct tctctgttat
    12301 tgtagtatga ggtgctgctt ctacacctat aacccctcct tttttagcac atgctattaa
    12361 tacttcatct ggagttagtc tctttgtgtt ccataaactt cttgccccaa catggcttaa
    12421 tattattggg tccttgctat attctattac atcaagtgta gtttgaactc caacgtgaga
    12481 gcaatctatt gccattccaa ttttattcat tctttcaaca gctttttttc cgaaatttgt
    12541 aagacctcca tctttctctt cttttagtcc tgaacctaat gcattggatt cgctgtatgt
    12601 tatacccatt aaccttatac caaatccata cagtatatct attctatcaa gttcattttc
    12661 tattggtgct gcaccttcta atgttggtat caatgctact ttaccttcac ttttagctct
    12721 gtatatatct tctacttttt cacactttat aacaaaatct tgatgagcta aatcacatag
    12781 tctcatgcct aaatctatta atatatcatc ccatttccac cctgaatttg atgttatcgt
    12841 acatacacca tccattaaat tatcaaatat agcatcataa tatcctcttg ataatgcttc
    12901 ataagcacat ctttgtttac cttccctatt atattcaaat atatctcttt ctaggtgttc
    12961 tggaaataat actggatgtt catgtaatga tataaatatt ttttcatcca ctatactttt
    13021 aaccttagct tcttgaatat catctaattc aataaggtat tctttaactc ttttatctcc
    13081 tttacacatc tcaatagatg aaatatcttt tcctttttct aaataactat aggcttcata
    13141 tcctttgtaa gctgacttca taacaacatc ctccaagttc tacaattttt tatatttttc
    13201 ctatgtagca attgatgtgc caaaatataa attttaattg tataataaat cttgcaaaaa
    13261 ataaaaacag tagtaaggtt gatatttact actgttttat taaaaataat cttcttagat
    13321 tatttattca atatttttga attatcttaa tcttaaagat aattttattt atttctatcc
    13381 attataggga tttttttagg gtgttttgcc tttttagtcc cctatatcac tcccttaatt
    13441 tttacatatg atataaagtt ctttccttca tcagttataa aactaccttg tttttttact
    13501 ccagataaaa gtaaaccatg gctctttaac ttatctattc tttttctaat ttggtctaca
    13561 cttaaaacta tatctttttc ttttaaaatc tcttgcagtt tatttcttcc taataatata
    13621 tttttattat tccaagtttc tatactggtc aatatacata tactttcctc aaagaaatta
    13681 gattgtttga aatctaagat aattgaatcg aaattttcat taactaaagt attgtttttt
    13741 tcaaatctaa attgttctgg caaatgctcg taatctactc tatcttcctc aactatagta
    13801 tcaatataat aaattaaatt tttaagctct cttacatttc ctggccattc atacaatgtt
    13861 aaagcttgca ttgattcctc tgtaaaacat ttgttgctat ttatttcatc taaaaaatac
    13921 ttacttataa gaggtatatc ttccttacgt tctctaagtc taggtatctc tatatgtaat
    13981 acattaatcc tataatacaa atcttctcta aaagaacctt ctttaatttt tttctttaaa
    14041 tctttatttg ttgcagctat aattctaaca tctattggta taattttact tccgcccatt
    14101 ctcataactt ctttttcctg taatactctt agcaatctct gctgtacatc taaagatata
    14161 tcacctatct catctaaaaa aattgttcct gtatgtgctc tttcaaatat acccattttt
    14221 ccacctttta ttgctcctgt aaaacttcct tcatcatagc caaaaagttc actttcaata
    14281 agtgtatctg acaaagaaga tagattaact gcaacaaatg gcttgttttt tcttagagat
    14341 tcattatgta ttgcctgggc aaatatttct tttcctgttc cattttctcc aagtatcaac
    14401 acagaaaagt ctgtagttgc tatttttctt gcaatattaa ttttttcttt tattatttta
    14461 ctctcaccta caacactctc aaatgtatac tttgatacaa aaccttttga ctgaagttta
    14521 ttttgaatct tgtcctcaag cacttgaatt gcacttatat ttttaatgct tataatactc
    14581 tttattcgtt cattattttc ataaacattt actttattaa ttatcagttt tttgttattt
    14641 aaatttacta cttcatcatt aacttcatct tcttgaaaaa atattttttt gacaacctta
    14701 tcactaaata agtccataaa gttattagat ataatttcat tttggtctat tccaactaga
    14761 ttagaaaata ctttgttgca atatataaat tttcctaatt tatcaacaga agcaatacct
    14821 tcatctataa tctccaaaaa actcttcatg gttttattca tagttgtata atacctatat
    14881 ccacttactg tatcttcatt gtatttttcc ttaataatat gtagtttatc tatagatata
    14941 tttagttttg taaagatttc tataactgta tttatatcaa ttaccttatc acctatgtca
    15001 attatttgct ttatgttttg tggtatgctg tttctgcttc ccgtaataat tccaatctca
    15061 catttagact tatcacaacc tggataatat ggtattagat taatatgaat taatcctagt
    15121 tttctaatta tcttggcagt ttcatctgct gactctttat aagcatctat aaccataaca
    15181 tcagaatcat tttctatact tattatctga tttatatttt caatatttat agtacgatgt
    15241 actattacaa taggtatatt tttatcaata ttattatgta tatattcctt tatatcattg
    15301 tctgaacata ctacaagctc atagcttgac agttcaatat ttatcctatt tacatatacc
    15361 aagttatcta tattacaaaa ttctccaaat atattttcca attgttttga tatatctata
    15421 tttattttgt catcagttga tacaaataaa atattatgtt ttttcatagt cccacctcct
    15481 gtttaacgag tatagaaagt aataatttgg tctattgtag cactttttaa aaaagtttta
    15541 aatcttaaaa atcaaatttt gtaccattta ctattatttt ccataataaa ataatgtaag
    15601 ctatcttttt tcacatataa tttaatttct aaaacggtta gtttgacatt att
//
LOCUS       DAYAPA010000102        15590 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.102, whole genome shotgun sequence.
ACCESSION   DAYAPA010000102 DAYAPA010000000
VERSION     DAYAPA010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15590
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.102"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            364..3267
                     /locus_tag="ACGI59_002853"
     CDS             364..3267
                     /locus_tag="ACGI59_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFG9740632.1"
                     /translation="MELRDKLVNIINTENKKDPLTDVQIAKFLSTTRENITNLRKELN
                     ISNSRQRRYPYLKSAISAILQKNKNISISEITRELMTEGFNISRRVVEELLPKESLEM
                     DVVEEDEEESNDPFETLIGNKGSLRNAVEQAKSAILYPPKGLPTLIIGESGVGKSLFS
                     RHMYEFAKQKKIVKESSNFVVFNCADYSDNPQLLLSLLFGYKKGAFTGAEYDTPGLVE
                     EADEGVLFLDEIHRLPPKGQEILFSILDRGKFRRLGETNAERKVSIMLIGATTENVET
                     NLLLTFRRRIPMLITLPPLHDRLLKEKIDLIYNIFQQECNRINAKIFVDKNVIEILAL
                     KKFSGNIGQLQNMIQVLCARAFMKFINSKDEDSESIVVVDINEVLKLKDSFKDAAFQE
                     IEYTEIRKYLKNAIFIPFNLEESSVKGSLLSDEYILPEDIYKTIEKKYYDLKSLDIED
                     IDIENILWTFILNRFADLKFNLDSNEEIFSMSDLNSFVSDSLVKLVKELMNDIVEKNM
                     NLEVNKNMFKYLAIHLEEAIKRIKLNQKIINVNLEKIKIDFSKEYEISKSFAIKIEES
                     IGIKISDDEIGFITLYIKSALKNEVKKDKVGLIIISHGRIATETVNVVKELLGVKFPV
                     AIDMPLDEKPINIYNKAVELSKIIDQGKGILFLVDIGSLTNIGQIVNKRTNISTKTID
                     RVDLLMALEATRKVSMGEEELDEIFFSLLKDRMGYNYELEKHIDKSNAIITLCLTGEG
                     TAKYISKTLEEKYDNTKCYQMSALDESLFCKIENLKETNNILAIVGTINPKIPGINFI
                     PYNKELFKNLDIYLSKSKQQDDRPIKSYERMLDEDLVIFEPDIYFKKDLLEYVCSILI
                     NKEYVEKDYLDSVLHREEMLPTYSKGAIGVPHGDSSTVNSTRFVFVKLKNPIDWGVGN
                     VNFIFMPVFQANDKEIVKNVLEILKDAEFMNNANKCFDKDAFRRIIFDKFKHL"
     gene            3300..3746
                     /locus_tag="ACGI59_002854"
     CDS             3300..3746
                     /locus_tag="ACGI59_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFG9740633.1"
                     /translation="MNVDLISLGLESNDYESVIEELGSIMCKKEYVKETYINAVLERE
                     RTLPTGLDIGDMCVAIPHTDSKHVNESNVAVGILKNPVKFNSMIDPKDRLDVELVFLL
                     AVKNPDSQVKLLKDLMSVFQNIKLLKNIKNASTKEEVAKLLDFIEI"
     gene            3869..4171
                     /locus_tag="ACGI59_002855"
     CDS             3869..4171
                     /locus_tag="ACGI59_002855"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFG9740634.1"
                     /translation="MKQFNVLSVCGSGTVTSSMVAGKLKEVLGNKGISITTTEARPTE
                     ALNLAQSGRFDFITFTSPLQTGDYGIPTINAFACLTGIGEEAFFEEVLTVIEGIDK"
     gene            4352..5758
                     /locus_tag="ACGI59_002856"
     CDS             4352..5758
                     /locus_tag="ACGI59_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFG9740635.1"
                     /translation="MLEFMKSIIDTFGSAIIVPIIIFIIAKIFKVTTKKSFLSAVYAG
                     VALQGFTLILNSFTPVITPVINRMVESTGVNLPVFDVGWQATSLVAFSTSAGMIYLGL
                     GILLQTILFLIKWTDVFQPSDLWNNYSYMVWGAMVIGVTGNFPLGIACMVLLNLYSLL
                     ISELVAKRWSSYYRYPNCTIIAMHNVEASVFAVFADPIYNKLGLNKIKLNPKELEKKL
                     GFLGEPITLGLFLGMFIGILGNMTRINTMEAWGEIMKVGISTSAVMAIFPKVASMFAQ
                     AFAPITEAARKIMQKAGNREWYIAVNDAVGYGEPATLISGLILIPIMLVIAMVLPGNK
                     VLPVVDLLAIPYMVQGLVAIHNGNIPKVLVSGIIWFGLGLYVCTSTAPLFTDMATNIG
                     VAIPAGAMLITSFNILGKPLMGLVFFAFLSANPIYIGLSVVIYFVLWALFRKNKTSIL
                     DYLEKQALKNVEEEPVAV"
     gene            6102..7160
                     /locus_tag="ACGI59_002857"
     CDS             6102..7160
                     /locus_tag="ACGI59_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFG9740636.1"
                     /translation="MGNKMRASVLYNVGDVRYEMVNIPEITDTQVLVNVKYVGICGSD
                     LPRSMVSGLSGNTKYPLILGHEFSGEVVKIGEKVKHINVGDRVAVAPLVPCGKCDYCN
                     EGNFGLCDDYNIIGTRVNGAFAEYVRVPEEHILKLPDTLDYETAAGIEPATIAYHGIS
                     KSNIRVGDSVVVLGCGPIGQFVIQWAKVFGASKIIAVDIFDEKLELSKLLGANYILNS
                     KEVNVIKEIKKITNGGADVVIETAGSRFTQEQSLFVAKKRGNIVFVGISHTELPLSAD
                     ATECILRGELTLKGSWNSYTSPYPGRAWTATLDFMEKGDIIFKPMISDKIGLNEVGDF
                     LSKMSKREINFNKILVEI"
     gene            7259..7945
                     /gene="araD"
                     /locus_tag="ACGI59_002858"
     CDS             7259..7945
                     /gene="araD"
                     /locus_tag="ACGI59_002858"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFG9740637.1"
                     /translation="MLENLKEEVLKANLELPKKKLITYTWGNVSGIDREKGLVVIKPS
                     GVEYSNMTVEDMVVVDLEGNIVDGKLRPSSDTPTHLVLYKEFEELGGIVHTHSSWATI
                     WAQIGKSIPSCGTTHADYFYGSIPCTRKMTREEICNEYEKETGNVIIEEFMGRNPIHC
                     PGIIVNDHGPFTWGKDANEAVHNAVVLEEVAKMAYYTELMSPDNIMDKVLMNKHFSRK
                     HGKNAYYGQK"
     gene            7963..8610
                     /locus_tag="ACGI59_002859"
     CDS             7963..8610
                     /locus_tag="ACGI59_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HFG9740638.1"
                     /translation="MLDSLKGKVVEIAIKAEHYGLCKEKTGNFSIRDKDTGYILITPS
                     GVGREELKVEHICVLDLDGNVIEVEKGIKPSSEVLMHIEIYKTRENINAISHTHSLYA
                     TAFSVAKKPIIPIVYESVNYGGYVDIASYERPGTLKLAKSVSKLLIDTDACLLERHGL
                     TTVGNDLDEALLKSRYVEEVAEIYYRSLVLNQFKELEVVDVDEFKSWKYPEEIKF"
     gene            complement(8783..10150)
                     /locus_tag="ACGI59_002860"
     CDS             complement(8783..10150)
                     /locus_tag="ACGI59_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HFG9740639.1"
                     /translation="MNLIDILNKVDAFIWGPPLLVLLVGTGILLTVKLGVVQITKLPR
                     ALKLIFSAENKGSGDVSSFAALCTALAATVGTGNIVGVATAIKAGGPGALFWMWIAAF
                     FGMATKYSEGVLAIKYRTKDKNGQVSGGPMYYIVNGMGEKWRPLAIFFAISGILVALL
                     GIGTFTQVNSITDAINNSFGIDPRITGVVLAVFVALVVFGGLKSISNVATKIVPFMAV
                     IYVVICGIILISFWNKIPETFMLIIKSAFTPTAATGGFLGATMSLAIRNGIARGVFSN
                     ESGLGSAPIAAAAAKTEWPAEQGLISMTGTFIDTIIICTLTGFSLVISGVWCSDLNGA
                     VMTQAAFNGAIPTFGPILLTVSLTLFAFTTILGWSYYGERCFEFLFGVKGMNGYRTVF
                     VAMVLLGAFLKLEVVWIIADIVNGLMAIPNLIALLALSPIIVSETKKYFEHINSPENQ
                     IKKNA"
     regulatory      complement(10340..10428)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            10856..12976
                     /locus_tag="ACGI59_002861"
     CDS             10856..12976
                     /locus_tag="ACGI59_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFG9740640.1"
                     /translation="MGKILVLAEKPSVGRDIAKVLGSKNEKNGYIEGPKYVITWALGH
                     LVTLADPESYGERYKSWSLEDLPILPKHLKTVVIKKSGKQFNTVKSQMNRNDIDEIVI
                     ATDAGREGELVARWIIEKSQVKKPIKRLWISSSTDKAIKDGFAKLRSGKEYENLYYSA
                     IARAEADWIIGINATRALTTKYNAQLSCGRVQTPTLAMLLKREEEIRNFKPKEYYGLE
                     LIATKGNSDIKFIWNDKNNNSSTFSKEKIESTLKKVKGVDIKISEVNKSYKKKYSPAL
                     YDLTELQRDANKMFGFSAKETLSIMQKLYEHHKVLTYPRTDSRYLTSDIVGTLKDRIK
                     AVNISDYSKICNRLLKSNIIANKSFVDNSKVTDHHAIIPTEQKVFMSDFSDKERKIYN
                     LVVKRFLAVLSPPFEYEQTTISATVEGEIFKAKGNKVINLGWKESYKDIEDEDEYESI
                     PNVLEKDVLKVKGLKITSGKTNPPPYLNEGTLLTAMEKNGLGTVATRADIIEKLFNSF
                     LMEKKNKDIYITSKGRQLLDLVPVDLKTPELTASWEKKLLDISKGKLSKNTFINDMKD
                     YSKDIVLEVKNSDNKFKHDNLTKNRCPECGKFMLEVNGKKGKMLICEDRECNTRKTIS
                     QVTNSRCPNCHKKLELRGEGEGRLFTCSCGYREKLSSFNKRKQEEKQKASKKDVNQYL
                     KNQNKNQETFNNPFAEALAKLKNK"
     gene            13080..14333
                     /locus_tag="ACGI59_002862"
     CDS             13080..14333
                     /locus_tag="ACGI59_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliS"
                     /protein_id="HFG9740641.1"
                     /translation="MDLSGIFKYYCKECENTWNNSSVELFENIETYSKDSQKKREKEL
                     DKLLNTISVHLERYPSDAVLRKMWVKKGEVFLQKTLEKENIFKLEKMDVEDRKKFLDI
                     TKQFIRDARKFDDDLPIGDIMQAMRNVWISNALQLLFGKEVYYSKANFAYSMLYPYTD
                     NYLDNTNIDKNDKILFNNWLEKRLLGEHIKSKDYHESKVSQMIDYIESVYPREKFTEV
                     YESLLLIFKSQVNSLKQHGKENHLCKEDLLSISIEKGGSSVLVDGYLISGLMTKEEIE
                     FCIGYGFLLQISDDLQDIKEDLKYNHKTIITEMSKEGTLDKVVNKLINFTIELIDSFK
                     INNKNKSVIAMIKNDCLMLILFSVVYNAEFFSVGYIKEVEKFIPYTIDYSLEIEEKIK
                     EKFKNIDVLNNENEYKEMIDIICAE"
     gene            complement(14487..15548)
                     /gene="ytvI"
                     /locus_tag="ACGI59_002863"
     CDS             complement(14487..15548)
                     /gene="ytvI"
                     /locus_tag="ACGI59_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
                     /protein_id="HFG9740642.1"
                     /translation="MDIQKQKNFIIKFTYVVLITSIVYIVLKFLLPLLMPFVISFIIA
                     SILRPIIRLITNNTNLNRTLISIVILLIFYGLCIFLLVSFGVKIFASVSDVFFRLPEI
                     YKSSIQPTLNTLFSKIDASTPNVNLALILGWDNISQSMMSLVASISTNALNAIASIAS
                     KTPAFMLKLIITLIASFFFTFDYQKIVNFILKQFPEKSQFMIINIKNSSINALLKLLK
                     AYAILLSVTFIELLIGLTILKVENAFTISVIIALVDILPVLGTGSILTPWMIISLING
                     NINLAIGLLILYIIITVVRQILEPKVVGHQIGLYPLITLMCMFVGAQLFGIAGLFGFP
                     IAATIIKNLHDNGIITAFK"
BASE COUNT         6199 a         1763 c         2681 g         4947 t
ORIGIN      
        1 ctatataagt atgtatacta tcatataaaa ataaaatctt aaaatagaaa gggttaaaaa
       61 cacagtttat tataattttt attgttgagt agatacgtac tattatatat gattgagcaa
      121 gtttttcaat cataaattta taaagtattt tgcattaaaa tttaaacaaa gaattaaatt
      181 atttaagatt atgaataact aatacattta gtaaattact aaatatacaa aatgtaaata
      241 cttatcaaaa tgtataaata cttatgacat ctagttactt acagggttta agtagaaaaa
      301 aatatcaaag taagtatggc atgatagttg cttatatatt tatcaagaaa ggagttatta
      361 agcatggagt tgagagataa acttgtaaac atcataaata cagaaaataa aaaagatcca
      421 ttaacagatg ttcaaatagc aaagttttta agtacaacta gagagaacat aactaatttg
      481 agaaaagaac ttaatataag taattcaagg caaagaagat atccttattt aaaatcagca
      541 atttcggcaa tattacaaaa gaataagaat atttctatat cagaaattac aagggaattg
      601 atgacagaag gctttaatat atcgagaaga gttgtggagg aacttttacc taaagaaagt
      661 ttagaaatgg atgtagtaga ggaagatgaa gaagaatcga atgacccatt tgaaactctg
      721 ataggtaata aagggagtct tagaaatgct gtagagcagg ctaaatctgc tatattgtat
      781 ccaccaaaag ggttaccaac gttgattatt ggggaaagtg gagttggtaa gagtttattt
      841 tctagacata tgtatgagtt tgcaaagcaa aaaaagatag taaaagagag ctccaatttt
      901 gttgtattta actgtgctga ttatagtgac aatccacaat tattattatc tttgttattt
      961 ggatataaaa aaggtgcatt tacaggtgct gaatatgata caccaggtct tgtagaagaa
     1021 gctgatgaag gagttttatt tttggatgaa atacatagat tgcctccaaa aggtcaagaa
     1081 atactatttt ctatcttaga taggggaaag tttaggagat taggtgaaac taatgctgaa
     1141 agaaaagtaa gtattatgtt gattggggca actactgaaa atgtagagac taatttatta
     1201 ttaacattca gaagaaggat accaatgcta ataactttgc caccattaca tgatagatta
     1261 ctaaaagaga aaatagacct tatatacaat atctttcaac aagagtgtaa tagaataaat
     1321 gcaaaaatat ttgtagataa aaatgtgatt gaaatacttg cactaaaaaa gtttagtgga
     1381 aatataggac aattacaaaa tatgattcag gttctttgtg caagagcttt tatgaagttt
     1441 ataaattcca aagatgaaga tagtgaatct atagtagtag tagatataaa tgaagtttta
     1501 aagcttaaag attcgtttaa agatgctgcc tttcaagaga ttgaatatac agagataaga
     1561 aaatatttga aaaatgctat atttatacca tttaatttag aagaatcatc agtaaaagga
     1621 agtcttttaa gtgatgaata tatattacca gaggacatat acaaaacaat tgaaaagaaa
     1681 tattatgacc taaaatccct agacattgaa gatatagata tagagaatat tttatggact
     1741 tttattttaa atagatttgc tgacttaaag tttaatcttg atagtaatga agaaattttt
     1801 tcaatgagtg atttaaatag ttttgtaagt gatagtttag ttaaactggt aaaagaactt
     1861 atgaatgata tagttgaaaa aaatatgaat ttagaagtaa ataaaaatat gttcaaatat
     1921 ctagcaatac acttagaaga agctataaaa agaattaagc taaatcaaaa gataataaat
     1981 gtaaatttag aaaaaataaa gattgacttt tcaaaagaat atgaaattag taaaagtttt
     2041 gcaataaaaa ttgaggaatc aattggtatt aagatttcag atgatgaaat tggatttata
     2101 acgctctaca taaagtctgc tctaaaaaat gaagtgaaaa aagataaagt agggttaata
     2161 ataatttctc atggacgtat agctacagag actgtaaatg tagttaaaga acttttaggt
     2221 gtaaagtttc ctgtggctat agatatgcct ttagatgaaa aaccaataaa tatatacaat
     2281 aaggcagtag aattgtcaaa aataatagac caagggaaag gcatattgtt tttggtggat
     2341 attggttcac ttacaaacat aggtcaaata gtaaataaga gaaccaatat aagtactaaa
     2401 actattgata gagtagacct cctaatggca ctagaagcaa ctagaaaagt ttctatggga
     2461 gaggaagaac ttgatgaaat atttttttct ttgctaaaag ataggatggg atataactat
     2521 gaattagaaa aacacataga taaatcaaat gcaattataa cattatgtct tactggagaa
     2581 ggaactgcta agtatataag taaaacgttg gaagaaaagt atgataacac taaatgttat
     2641 caaatgagtg cattggatga aagtttattt tgtaaaattg aaaatctaaa agagacaaac
     2701 aatatattag ctattgttgg gacaataaac cctaagatac caggtataaa ttttataccc
     2761 tataataaag aacttttcaa aaatttggat atctatctat caaaatcaaa acaacaagat
     2821 gacagaccaa ttaaatcgta tgaaagaatg cttgatgagg atttagtgat ttttgaacca
     2881 gatatatatt ttaaaaagga tttattagaa tatgtatgtt caattcttat aaataaagaa
     2941 tatgtagaaa aagattactt agattctgtt ttacatagag aagaaatgct tccaacatat
     3001 tctaaaggag ctataggtgt gcctcatgga gattcttcta ctgtaaatag tacaaggttt
     3061 gtttttgtta aactaaagaa tcctattgat tggggtgtag gtaatgtaaa tttcatattt
     3121 atgccagttt ttcaggctaa tgataaagag attgttaaaa atgttcttga gatactaaaa
     3181 gatgcagaat ttatgaacaa tgccaataag tgttttgata aagatgcttt tagaaggatt
     3241 atatttgata aatttaaaca tctgtagcca tagcaaaaaa attgagagga gttagagtaa
     3301 tgaatgtaga tttaatatca ttgggtttag aatctaatga ttatgaaagt gttattgaag
     3361 aattaggaag tataatgtgt aaaaaagaat atgtaaaaga aacatacata aatgctgtct
     3421 tggaaagaga aaggacatta ccaacaggtc tagatattgg agacatgtgt gttgctattc
     3481 cacatacaga ttcaaagcat gtaaatgagt ctaatgtagc tgtagggata ttgaaaaatc
     3541 ctgtaaagtt taattcaatg atagacccaa aagatagact tgatgtagaa cttgtatttt
     3601 tattagcagt aaagaaccca gactcacaag taaaactgtt aaaagattta atgtcagttt
     3661 ttcaaaatat aaagttatta aaaaatataa aaaacgcatc aacaaaagaa gaagtagcta
     3721 aattattgga ttttatagaa atttaatgta ggagaatttt ataaataaaa aattaaaatt
     3781 taaacaatca aagtaagtat tattttttta ataggtttaa tcttatataa aataaaaatt
     3841 tagagtgaaa aattaggagg aaattattat gaaacaattt aatgtattat cagtttgtgg
     3901 ttcagggaca gttacatcat ctatggtagc aggtaaatta aaagaagtac taggtaataa
     3961 ggggatttct attactacaa cagaagctag accaacagaa gcactaaatt tagcacaatc
     4021 aggaagattt gactttataa catttacgag tccacttcaa acaggcgatt atggaatacc
     4081 aactataaat gcatttgctt gcctaactgg tataggggaa gaggcattct ttgaagaagt
     4141 tttgactgtt attgaaggga tagataagta agttttggag acgtgaattt atagtattta
     4201 aatcaatgat ttagatacta taataagcta atattaacta aatatttgtg gaaaaaacat
     4261 tttatatgtt gtcataaatt aaaaaattat aaaaatagtc acttaagtat taaattaagc
     4321 ttataaatat ttataagtag gagggaaaaa gatgttagaa tttatgaaat ctataatcga
     4381 tacatttggt tctgcaatta tagttccaat tataatattc ataatcgcaa agatatttaa
     4441 agtaactact aagaaatcgt ttctttctgc tgtgtatgca ggagttgctt tacaaggttt
     4501 tacactgatt ttaaattcat ttacaccagt aataacacca gttatcaata ggatggttga
     4561 gagtacagga gtaaacctac cagtttttga tgttggttgg caagcaacat cattggttgc
     4621 attttcaaca agtgcaggta tgatatattt aggtcttggt atattattac aaacaatttt
     4681 atttttgata aaatggacag atgtgtttca accatcagat ttatggaata actattctta
     4741 tatggtatgg ggagcaatgg ttataggagt aacaggaaat tttcctttag gtatagcatg
     4801 tatggtttta ttaaacctat atagtttatt aatatcagag ttagttgcaa agagatggtc
     4861 tagttactat agatatccta attgtacaat aatagctatg cataatgttg aggcttctgt
     4921 gtttgcagta tttgcagacc ctatatacaa taaattggga ttaaataaaa taaaattaaa
     4981 tccaaaagaa ttagagaaga aattagggtt tttaggtgaa ccaattactt taggtctttt
     5041 tttaggtatg tttataggta tattaggtaa tatgacgaga ataaatacta tggaagcttg
     5101 gggagaaata atgaaagtag gtatatctac aagtgcagtg atggcaattt tccctaaagt
     5161 agcaagtatg tttgctcaag catttgcacc tataacagaa gcagctagaa agattatgca
     5221 aaaagctgga aaccgtgagt ggtacatagc agtaaatgat gctgttggat atggtgaacc
     5281 tgcaacttta atatcaggtc ttatattaat tccaataatg cttgtaatag ctatggtact
     5341 tcctggtaat aaggtattac cagttgtgga tttattggca attccatata tggttcaagg
     5401 tttagttgcc atacataatg gtaatatacc aaaagtatta gtttcaggta taatttggtt
     5461 tggattagga ttatatgttt gtacatctac agcaccatta tttacagata tggctacaaa
     5521 tataggtgta gctatacctg caggagcaat gttaataaca agttttaata tactaggaaa
     5581 acctttgatg ggattagttt tctttgcatt tctaagtgca aatccaattt atataggtct
     5641 ttcagttgta atttactttg ttctatgggc attgtttaga aagaacaaaa caagtattct
     5701 tgattattta gaaaaacaag cacttaaaaa tgtagaagaa gagcctgtag cagtttaagt
     5761 ttataaaaat gaaataaaaa taattaaagg agtttataat tgattttata taagtatcag
     5821 ataatacctg agaaatattt aaatgaaata tagacttctt tttatttgat aatatttgta
     5881 attataatgc attgtatttt tatagttgag ttttctgtat aacctgctat ttaaaacata
     5941 ctcttttata tttatacagt aatgtggttt aattttataa aatgtaatct ctactggtaa
     6001 aagactttgt atataaggca tttgcctgaa taaaccattt atcttttgta tggcatagat
     6061 attgctatat agaagtatat agcatgaaag gagatattta tatgggaaat aaaatgagag
     6121 catcagtatt gtataatgta ggtgatgtca gatatgaaat ggtaaatata ccagaaataa
     6181 cagatacgca agtgcttgta aatgtaaaat atgttgggat atgtggttca gatttaccta
     6241 ggtctatggt aagtggtttg agtggaaata caaaatatcc attgatttta gggcatgaat
     6301 tttctggtga agttgttaaa attggagaaa aagtaaagca tattaatgta ggagatagag
     6361 tagctgttgc accattagta ccttgtggaa aatgtgatta ttgtaatgaa ggtaattttg
     6421 gcttgtgtga tgattacaat ataattggaa caagagtaaa tggagcgttt gcagaatatg
     6481 taagagttcc agaagaacat attttaaaac taccagatac attggattat gaaactgctg
     6541 ccggaattga accagcaact atagcttatc atgggatttc aaagtctaat attagagtag
     6601 gagatagtgt agttgtgtta ggatgtggtc caataggtca gtttgttata caatgggcaa
     6661 aggtgtttgg tgcatctaag attatagcag ttgatatttt tgatgaaaaa ttagaacttt
     6721 ctaaacttct aggagcaaac tatatactta actcaaaaga agtaaatgta atcaaagaaa
     6781 taaaaaaaat aacaaatggt ggtgctgatg ttgtaataga aactgctggt agccgattta
     6841 cacaagaaca atctctattt gtagcaaaaa agagaggaaa tatagtgttt gtaggtatat
     6901 cacacacaga attaccactt agtgcagacg ctactgagtg tatattacgt ggagaactta
     6961 ccttgaaagg ttcttggaat tcatatacaa gcccttatcc tggaagagca tggactgcaa
     7021 cattggattt tatggaaaaa ggtgacatta tttttaaacc catgatttca gataaaatag
     7081 gtcttaatga agtgggtgac tttttaagta aaatgagcaa gagagaaatt aatttcaata
     7141 aaatcctagt agaaatataa aaatttaata aaaaattcta aaagcaatta aattttataa
     7201 ctatgacatt aaataaaata aaaataattt tcttaaataa aatataggag gaagaaaagt
     7261 gttagaaaat ttaaaagaag aagtattaaa agcaaactta gaattaccta agaaaaagct
     7321 aattacttat acttggggaa atgtaagtgg gatagataga gaaaaaggat tagtggtgat
     7381 aaagccaagt ggtgtagagt atagtaatat gactgtagaa gatatggttg tagttgattt
     7441 agagggaaat atagttgatg gcaaattaag accttcttca gataccccga ctcatctagt
     7501 tttatataaa gagtttgaag aattgggggg aatagtacat acacattcaa gttgggcaac
     7561 aatttgggct caaataggaa aatctatacc atcttgtggt actactcatg cagattattt
     7621 ttatggaagt ataccatgta ccagaaaaat gactagagaa gaaatttgca atgaatacga
     7681 aaaggaaact ggaaatgtaa tcattgaaga atttatggga agaaatccaa ttcattgtcc
     7741 aggaataatt gtaaatgacc acggaccttt tacatgggga aaagatgcaa atgaggctgt
     7801 acacaatgca gtagttttag aagaagtcgc aaagatggct tattatacag agttaatgag
     7861 tcctgataat attatggata aggttcttat gaataagcat ttttcaagaa aacatggaaa
     7921 aaatgcatac tatggtcaaa aatgattatc tggaggtgaa ttatgctaga ctctttaaaa
     7981 ggtaaagttg tggaaatagc aattaaagca gagcattatg gtttatgtaa agaaaagact
     8041 ggtaatttta gtattagaga taaggatact ggatatatac ttataacccc atcaggtgta
     8101 ggaagagaag aattaaaagt tgaacatatt tgtgtattgg atttggatgg aaatgtaata
     8161 gaagttgaga aaggtattaa gccaagtagt gaggtattga tgcatattga aatatacaaa
     8221 acaagagaaa atataaatgc catatcacat actcattctt tatatgcaac agcattttca
     8281 gtagcaaaga aaccaattat accaattgta tatgaatcag ttaattatgg cggttatgta
     8341 gatattgctt catacgaaag accaggaact ttaaaattag ctaagagtgt ttcaaaatta
     8401 ctgatagata cagatgcatg cttattagaa agacatgggt tgacaactgt aggtaatgat
     8461 ttagacgaag cattattaaa atcaagatat gtggaagagg tagcagaaat atactatagg
     8521 tctttagtat taaatcaatt taaagaactt gaagtagtag atgttgatga gtttaaatct
     8581 tggaaatacc cagaagaaat aaagttttaa tctaaataat tttcattgaa gtggttaatt
     8641 aaaatatatg tattatttat atcaattact atgttttatt atgttaatta ccgtattttg
     8701 caaatatatt tattacattt tttcaaaaaa atatagaatc cgaaagccat ataaaattga
     8761 cttgggattc tatacttttt atttatgcat ttttctttat ctgattttct ggagaattta
     8821 tatgttcaaa atatttttta gtttcagata ctattatagg tgataatgca agtaaagcta
     8881 taaggttagg tattgccata agcccattta caatatcagc aattatccaa acaacttcta
     8941 actttaagaa agcacctaat aacaccatag ccacaaatac agttctatac ccattcatgc
     9001 cttttacacc aaacagaaat tcaaaacatc tttctccata ataactccat cccaaaattg
     9061 tagtaaatgc aaataatgtt aaactaactg taagtaatat tggtccaaat gtaggaattg
     9121 ctccgttaaa agctgcttgt gtcataacag ctccatttag gtctgaacac cacactcctg
     9181 aaataacaag agagaatcca gtaagagtac agattataat agtatctata aaagtacctg
     9241 tcatagatat aagaccttgt tccgctggcc attcagtttt agcagcagct gcagctattg
     9301 gagcactacc tagaccagac tcatttgaaa atacaccacg agcgatacca tttctaattg
     9361 ccagtgacat agttgctcct aaaaaaccac cagtagcagc agtaggtgta aatgcacttt
     9421 taattattaa cataaaagtt tcagggattt tattccaaaa agatattaaa atgattccac
     9481 agattacaac atatataact gccataaaag gtactatttt agtagctaca ttagatatac
     9541 ttttaagtcc tccaaataca actaaagcta caaatactgc taatacaacc cctgtaattc
     9601 tagggtctat cccgaaacta ttatttattg catctgttat tgaatttact tgagtaaaag
     9661 taccgattcc taaaagtgca actagtatac cacttatagc gaaaaatatt gcaagaggtc
     9721 tccatttttc tcccatacca tttactatgt aatacattgg cccaccagac acttgaccat
     9781 ttttatcttt agttctgtat tttattgcaa gaacaccttc tgaatactta gtagccattc
     9841 caaagaatgc tgctatccac atccaaaaaa gtgcaccagg tccaccagct ttgatagctg
     9901 tagcaacacc aacaatatta ccagtaccaa ctgtagcagc tagagcagta cataaggctg
     9961 caaaacttga aacatcacca ctacctttgt tttcagcaga gaatattaac ttaagagctc
    10021 ttggcagttt agttatttga acaactccaa gcttaacagt aagtagtata ccagtaccaa
    10081 ctaatagtac cagtaggggt ggtccccaaa taaaagcatc aactttgttt aaaatatcaa
    10141 ttagattcat aaaaacactc cctcattttt tttgctttac ataatcaata gaatttttaa
    10201 tgaagtaatt gacaggaaat aagtataatt tattaataat ttctagtgta ataaaaaaag
    10261 ctaaatctaa aaaagattca gctctggatg tacactaatt agcaaccaaa ataaaagtat
    10321 tagtaaatac tgtttttggt agataatccc tgtccttttg cctgagagat tgagcataaa
    10381 agccttgccc cttcggcgcc cataatgggt ctctccagag ttccgtaaat tttacagtcc
    10441 tacttttaaa agtacctgag agtgttactc cttcggtgtg ctatatagca actcttctat
    10501 aaaatcttaa ccggatattg atttgtcata gcttattgta ttaataatat taatataagt
    10561 ttttatgttt tgtcaactaa aaaattaaaa aacaaagaaa aaatataaaa atgtaagcct
    10621 atttacgaat tataaactta gaaagatgag taatatacgt gaaaatgaat tttgtaatac
    10681 acatacaaat gaagtgtaaa ataagtttta aataaaaaaa taataaaatc ttaaataatt
    10741 aattagatat gattaaatta tggtaatatt atagttacga tattaagaaa agttctattt
    10801 agttagattt taagcgtaat atgagattat gtatagagaa aggatgtatt tttagatggg
    10861 aaaaatatta gtgctagcag aaaaaccttc tgttggaaga gatatagcaa aagttttagg
    10921 gtcaaaaaat gaaaaaaatg gttatataga ggggccaaag tatgtgataa cttgggcact
    10981 aggtcatttg gttacacttg ctgaccccga aagttatgga gaaagatata aatcatggag
    11041 cttagaagac ctgccaatac ttcccaagca ccttaagact gttgtaataa aaaagagtgg
    11101 aaagcagttt aatacagtta aatcgcaaat gaatagaaat gatatagatg aaatagttat
    11161 agcaacagat gcaggtcgtg aaggagaact tgtagcaagg tggataatag aaaaatcaca
    11221 ggtaaaaaaa cctataaaac gtctttggat ttcttcaagt acagacaaag caataaaaga
    11281 cggatttgca aaattaagaa gtggaaaaga atatgaaaat ttgtattact cagcaatagc
    11341 acgagcagaa gctgattgga ttattggaat aaatgcaacc agagctttga caacaaagta
    11401 caatgcacag ctttcttgtg gaagagtaca aactcctact cttgcaatgt tgcttaaaag
    11461 agaagaagaa ataagaaatt tcaaacctaa agagtattat ggattagaat taattgctac
    11521 aaagggaaat tcagatataa aatttatttg gaatgataaa aacaataatt cttcaacttt
    11581 tagtaaagaa aagatagaat ccacattaaa aaaagtaaaa ggtgtagaca taaaaatatc
    11641 tgaagtaaac aagtcatata agaaaaaata ttcacctgca ctctatgatt taacagaatt
    11701 acaaagagat gcaaataaaa tgtttggatt ttcagcaaaa gaaactttat ctataatgca
    11761 aaaactgtat gaacatcata aagtacttac ttatcctaga actgattcta gatatttaac
    11821 atcagatatt gttggaacat taaaagatag aataaaagct gtaaatatta gtgattattc
    11881 aaagatttgt aatagattat taaaaagcaa tattatagct aataaatcgt ttgttgacaa
    11941 ttcaaaggta acagaccacc atgcaataat accaacagaa cagaaggtat tcatgagtga
    12001 ttttagtgat aaagagcgaa aaatatataa tcttgtagta aaaagatttt tagcagtact
    12061 atctcctcct tttgaatatg agcagacaac tataagtgca actgtagaag gagaaatatt
    12121 taaggctaag ggaaacaaag taataaattt aggttggaaa gaaagttata aagatattga
    12181 agatgaagat gagtatgaaa gtataccaaa tgttttagaa aaagatgtac ttaaggtcaa
    12241 gggattaaag ataacttcag gaaaaacaaa tccaccacca tacttaaatg agggaacatt
    12301 attgactgct atggaaaaga atggtctagg gacagttgct acaagagcag atataataga
    12361 aaaattattc aattcatttt taatggaaaa gaaaaataaa gatatatata taacatcaaa
    12421 aggaagacaa ttattagatt tagttccagt agacttaaag acaccagaac ttacagcttc
    12481 atgggaaaag aaattattgg atatttcaaa gggaaaactt agtaaaaata cattcataaa
    12541 tgacatgaaa gattattcaa aagatattgt actagaagta aaaaatagtg ataataaatt
    12601 taaacatgat aacttaacta aaaatagatg tccagaatgt ggtaaattta tgttagaggt
    12661 aaatggtaaa aaaggtaaga tgttgatttg tgaagataga gagtgtaata ctagaaaaac
    12721 tatatcacaa gtaaccaatt caagatgtcc taactgccat aaaaaattag aacttcgtgg
    12781 agaaggtgag gggagactct tcacttgtag ttgcggatac agagaaaagt tgtcttcttt
    12841 caacaagaga aagcaagaag aaaaacagaa ggcatcaaaa aaagatgtta atcaatattt
    12901 aaaaaatcaa aataaaaatc aagagacatt taataatcca tttgcagaag cattagctaa
    12961 gctaaaaaac aaataatttt aaagttatta ttatacacaa ttataacttt aactgtatat
    13021 tagttatatg tactgatata atatagtgta tagtttaggc atgaaagtag ggctgttaaa
    13081 tggatttaag tggcatattt aaatactatt gtaaagagtg tgaaaataca tggaataatt
    13141 cgagtgttga attatttgag aatatagaaa cgtatagtaa agattcacaa aaaaagaggg
    13201 aaaaagaatt agataaattg ctaaatacaa tatcagttca tttagagagg tatccaagtg
    13261 atgctgtatt gagaaaaatg tgggtaaaaa agggcgaggt tttcttacaa aagacattgg
    13321 aaaaagaaaa tatttttaag ttagaaaaaa tggatgtaga ggatagaaaa aaatttttag
    13381 atataacaaa acagtttatt agagatgcta gaaaatttga tgatgattta cctataggtg
    13441 atattatgca agctatgagg aatgtatgga tttcaaatgc attacaatta ttatttggta
    13501 aagaagtata ttattcaaaa gctaactttg catatagtat gttatatcca tatactgata
    13561 attatttaga caatacaaat atagataaaa atgataagat tttatttaat aactggttag
    13621 aaaaaaggct cctgggagaa cacattaaat ctaaggatta tcatgaaagt aaagtatctc
    13681 aaatgataga ttatattgaa agtgtatacc ctagagaaaa gtttacagaa gtttatgaat
    13741 cgttattatt aatatttaaa agtcaagtaa atagtttaaa acaacatggt aaggaaaatc
    13801 atttgtgtaa agaagattta ttatccattt ctattgaaaa aggaggttca tccgttttag
    13861 tagatggata tttaataagt ggattgatga caaaggaaga aatagagttt tgtataggat
    13921 atggattttt attacaaata tctgatgatt tacaagatat aaaagaagat ttaaaataca
    13981 accataaaac tattattaca gagatgtcaa aagagggtac tttagataaa gttgtaaata
    14041 aactaataaa ttttactatt gagttaatag atagttttaa aattaataat aaaaataaat
    14101 ctgtaatagc tatgataaag aatgattgct taatgttaat tttattttct gtagtttata
    14161 atgctgaatt tttttctgta ggatatataa aagaagtaga gaaatttatt ccatatacaa
    14221 tagattattc attagagatt gaagaaaaaa taaaagaaaa atttaaaaat atagatgttt
    14281 taaataatga aaatgaatat aaagaaatga ttgatattat ttgtgcagag tagaaaaaaa
    14341 gggagtgtct tagaatagac tttttagttt atgaggcact ctttttgtat aaaatcaaat
    14401 gtattttcat cattttaaca attaaaaaga gcttatccct atttgagaaa gcccttttta
    14461 atttaaataa aaatgtcaag gttaaactat ttaaatgcag taataatccc attatcatgt
    14521 aagtttttta tgattgtagc tgcgataggg aatccaaata atccagctat tccaaatagt
    14581 tgagctccaa caaacataca cattaatgtt attaaaggat ataggccaat ttggtgtcct
    14641 actacctttg gttctaatat ttgtctaaca acggttataa ttatgtatag tattaaaagt
    14701 cctattgcta aattaatatt tccattaatg agtgagataa tcatccaagg tgtcaaaata
    14761 cttcctgtac ccaagactgg caaaatatca acaagtgcta tgatgacaga tatagtaaat
    14821 gcattttcaa cctttaggat agtaagtcca ataagtagtt ctatgaatgt cactgatagt
    14881 agtattgcat aggcttttag tagttttaaa agagcattaa tactgctatt ttttatattt
    14941 attatcataa attgactttt ttctggaaat tgttttaaga taaagtttac tattttttga
    15001 tagtcaaaag taaagaaaaa agaagcaatt aaagtaataa taagttttaa cataaatgca
    15061 ggtgttttac ttgcaatact agcaatagca ttgagtgcat ttgttgaaat agaagctact
    15121 aaagacatca tagattgact tatattatcc caaccaagta ttaatgctag gttaacatta
    15181 ggtgtactag catctatctt tgaaaataaa gtatttagcg ttggttgtat acttgattta
    15241 tatatttcag gaagtctaaa aaatacatca cttacacttg caaatatctt cacaccaaag
    15301 ctaactaata aaaatataca caacccataa aaaattaaaa gtattactat agaaataagt
    15361 gtacgattta aatttgtatt atttgtaata agtcttataa taggtcttaa gatggatgca
    15421 atgataaaac tgattacaaa aggcattaat aatggcaata agaattttaa tacaatatat
    15481 acaatacttg ttattaaaac tacatatgta aatttaatta taaaattctt ttgcttttga
    15541 atatccataa tataaaacct ccaatgtatt tgatattatt ctaaatatgg
//
LOCUS       DAYAPA010000103        15543 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.103, whole genome shotgun sequence.
ACCESSION   DAYAPA010000103 DAYAPA010000000
VERSION     DAYAPA010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15543
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.103"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            301..1233
                     /locus_tag="ACGI59_002864"
     CDS             301..1233
                     /locus_tag="ACGI59_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006441199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rossmann-like and DUF2520 domain-containing
                     protein"
                     /protein_id="HFG9740643.1"
                     /translation="MGFIGSGKVGTSLANYFLSKGYCVSGFYGRNQLSLLESTNLTKT
                     KIYSNLKDIIYENDILFITTSDDSIEIIDKKLSKFNLKHKYICHTSGSLKSSILFCSK
                     KAGALIYSIHPIFAFSNKNTDIKKMKDICFSIEGDNEQDDLVIQNFIDDLGNQFFIRD
                     KDTSSTYHLANVLVSNLVLSLLDIGVSYFIKLGLSEEESLKAISPLVKKNIENILDNG
                     FTKALTGPVSRGDITPIRKHLSVLDKKDEDIYKILSLNLLKIIALGNDNSLKGIKNIT
                     IENAIENLISKSEKYKEIYKLLGGKNDEKYHTDF"
     gene            1208..2035
                     /gene="panB"
                     /locus_tag="ACGI59_002865"
     CDS             1208..2035
                     /gene="panB"
                     /locus_tag="ACGI59_002865"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HFG9740644.1"
                     /translation="MKNTIQTFKNAKYEGKKLSMLTAYDYSIAKIMDECDINGILIGD
                     SLGMVIKGEENTLSVTIDEIIYHTKAVKNGVKNALIVSDMPFLSYHVSIEDAVKNAGR
                     LIKEGGAHAVKLEGGSNVIKQIESIVNAQIPVMGHLGLTPQSVNSFGGFKVQGNTSET
                     ARQLIEDAKLIEKAGAFSIVLEGVPTKIAEMVTNSISIPTIGIGAGINCDGQILVYQD
                     MLGMFGDFVPKFVKQYANIGDIIKDSIKNYILEVNTGAFPQEKHSFSINESELEKLY"
     gene            2054..2902
                     /gene="panC"
                     /locus_tag="ACGI59_002866"
     CDS             2054..2902
                     /gene="panC"
                     /locus_tag="ACGI59_002866"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HFG9740645.1"
                     /translation="MLVKEIKLLRNIIKDWRKHGYSIGLVTTMGFLHEGHQSLIKKAV
                     KENDKVVVSIFVNPTQFGPNEDFNSYPRDIDKDFKYCMDSGATVVFNPSPEEMYLKGN
                     CTTINVSGLTDFLCGAKRPVHFGGVCLVVSKFLNIVTPDKAYFGEKDAQQLAVIKRMV
                     KDLNIDTEIIGCPIIRENDGLAKSSRNTYLSEEERKSALILNKSLSLAKEELVKGNLN
                     PENIKELITAKINSEHLAKIDYVEIVDSETLQPVKQIEHSILVAIAVFIGKTRLIDNF
                     TFELNI"
     gene            3313..3495
                     /locus_tag="ACGI59_002867"
     CDS             3313..3495
                     /locus_tag="ACGI59_002867"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9740646.1"
                     /translation="MFKDKIDECVHIMTAYIASLKEYYSFIETQIGDFIKKYGEDVVE
                     LCLHRVMILLCECGLA"
     gene            4322..5236
                     /locus_tag="ACGI59_002868"
     CDS             4322..5236
                     /locus_tag="ACGI59_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2935 domain-containing protein"
                     /protein_id="HFG9740647.1"
                     /translation="MIDNQKYVILSLELHLFFSRIMKEHALFLEAGFTNKNYNLAMEA
                     DHYKKQFEDLLSYTVSASNGIIRPDILYSEELVTTLTSVAEQKTEEFTGIEINKNITT
                     RELNLQSGVNPQVGQDLVNYVAQLNSDAIRLLDGLINFKERVLDGVLSCTIFTSNYPL
                     LLEHIIHEANLYRSYVVDLENKIDIESKNAKEIELFWDHIMMEHALFMRGLLDPSEGE
                     LINTSNDFAIKFNELIEKTNEMTDSNIKNITEETLNETVEFKDFKEAGASGIEQCKIK
                     SIILPLLADHVLREANHYIRILESYKNM"
     gene            5626..6273
                     /locus_tag="ACGI59_002869"
     CDS             5626..6273
                     /locus_tag="ACGI59_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HFG9740648.1"
                     /translation="MIRQTPLEKRYDKLGPHIVTALKKRYFDAYYCKTRNEALQQILD
                     LIPQNHVVSWGGSETLKEMGVQTAVREKGFKVIDRDLAKTPEERTEIMRQALLCDTFL
                     MSSNAISEDGQLFNIDGNGNRVAAMIFGPKNVIVVAGMNKIVKNIDDAIQRTRTIASP
                     AVVQRFQDVNTPCYINGSCIDCTSPESICAYMVTTRISRPAKKIKVILVGENLGL"
     gene            complement(6440..7009)
                     /locus_tag="ACGI59_002870"
     CDS             complement(6440..7009)
                     /locus_tag="ACGI59_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9740649.1"
                     /translation="MKKFILCIIMFAISVLVLAGCTNDNKIKNEVDSMFNSIKTQDAD
                     TVDKYFPKSGLGNLIYEDKEGFKMYSKNMTWEISDIKNNKDKVTVDLKINNTDMVSIL
                     KKNPPKLGEMMPNPTMKDIDSAKKKEFNITLELVKSGDNYVCKVNQESAMKLLNVITG
                     GGIDYLADYNKDYYKQLDEEQKNYENKNS"
     gene            complement(7014..7913)
                     /locus_tag="ACGI59_002871"
     CDS             complement(7014..7913)
                     /locus_tag="ACGI59_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFG9740650.1"
                     /translation="MKLLSKVNNNIRLITQVAFTALTNGYVNGFLEGTIYGGESKKLC
                     VPGLNCYSCPGALGACPIGSLQAVLSSREYKFSFYVVGFLMAFGAFFGRFVCGWLCPF
                     GLVQDLLHKVPFPVKIKKVYGDKYLKYLKYIVLIIFVIILPVAIVNVAGGGNPWFCKW
                     ICPSGTLLGGIPLILGNENLRESIGFLFSWKLSILLLILLMSIITYRPFCKYICPLGA
                     IYGFFNPISIYRFKIDKDKCTNCTACQTKCKLDIPIYKDVNSPDCIRCGECIKVCPQN
                     AITTTFSKDKKENNICAKKYRGI"
     gene            complement(7924..8073)
                     /locus_tag="ACGI59_002872"
     CDS             complement(7924..8073)
                     /locus_tag="ACGI59_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD1871A family CXXC motif-containing protein"
                     /protein_id="HFG9740651.1"
                     /translation="MSNFIKDNKKGISIIAISIIFIALGTYRGETNTVLTKAIMICLE
                     CIGIG"
     gene            complement(8066..8650)
                     /locus_tag="ACGI59_002873"
     CDS             complement(8066..8650)
                     /locus_tag="ACGI59_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HFG9740652.1"
                     /translation="MKRFFIVVLGCICVLGMISGCSKKESKPVEKNKNNSEWFSNLDT
                     EDVDGNKVTKEIFSNKELTLINVWTTWCGPCVGEMPELEALSKEYESNNSNVAIKGLV
                     VEVDKTDMRTGLSNEEKNLVKDIMKKSDATYQQLTVSEELKKTDFKRVMEFPTTYFVD
                     KKGNFVGEKVVGANSKEDWKKIIDERLKMVKANE"
     gene            9039..10364
                     /gene="cdeA"
                     /locus_tag="ACGI59_002874"
     CDS             9039..10364
                     /gene="cdeA"
                     /locus_tag="ACGI59_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter CdeA"
                     /protein_id="HFG9740653.1"
                     /translation="MENLFTRKFTTFEFLKFVSPAIISMIFISLYTIIDGIFVSTLVG
                     SDALASINIVLPIINLVCGFGIMMATGGGAIVSIRMGENRQDEANSTFSFIVLFSLIV
                     GILFTVISYFFIKEISILLGATDKLLPYCITYGKVMILCTPFYILKFIFEYFARTDGN
                     SKFSLFLSVIGGVTNIILDYVFIKYFGMGLLGAAVATAIGIILTCVLGIIYFLSNKST
                     LKLRKPKTDFRLIRDTMINGSSEMVTELSTGITTFLFNVVALKLAGENGLAALTIVLY
                     AHFLMTSVYLGFAAGVSPLISYNFGAENSDKLKETFKHSLKFIFVSSLLVFIFALVFA
                     PFIVRVFVSPDNTVFKLALQGLKIFAFAFLFVGINIFASGFFTAFHNGKISAIISFSR
                     AFVFIIIGIIILPPMLNMTGLWLTVPFAEVITIFISILFIKKYKCRYKY"
     gene            complement(10459..11253)
                     /locus_tag="ACGI59_002875"
     CDS             complement(10459..11253)
                     /locus_tag="ACGI59_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9740654.1"
                     /translation="MNELKPYIKFFNKNTKLYIIIFMIVGIVMNILPVFILKIFGEAS
                     KTNLSTIVNPILTYITILLFVYGVNIANKDFSGALSIRADRKSCIKAIVIDLIILSFV
                     ISILGIVLIFLSKLFIEIITGYSIELSVLLKRDMIWTSISALIDPSTSSMPLTYLNYF
                     VQICINEICIGFIGVLLGAFTYRVRKVTSITILIGLPILMIIYIVNFATKNMHKMLLY
                     LEKIIYSFQNPFILFGTKVGIIVICIIFIILLLRKAPIKEYANDLL"
     gene            complement(11254..12129)
                     /locus_tag="ACGI59_002876"
     CDS             complement(11254..12129)
                     /locus_tag="ACGI59_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFG9740655.1"
                     /translation="MSSSIKIENLSHSFKKQNIFNNMSLSFEKNKIYGLLGKNGAGKT
                     TLLNIMVNQLIQNEGEIEILGKNPKEDIKVLEEVCIVREKEFYSPDFKVGQIFEFSSS
                     FYKSYDKGLEEELCKYFDLNTKKKYKQLSRGMKTILSNIIGICSNSAITIFDEPTIGL
                     DAVNRQEFYNIILDSYIKNPRTIIISTHLIDEIDDLLEHVIILNEGKIIIDEEIDVIK
                     QKAHYITGNKEELEKLECIKNIKPKKAFGNAVAYFYYGDFSESDEKILKNSNIDVGYI
                     GLQDMFVNMTKKEEL"
     gene            complement(12133..12501)
                     /locus_tag="ACGI59_002877"
     CDS             complement(12133..12501)
                     /locus_tag="ACGI59_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFG9740656.1"
                     /translation="MKLVLNNEEPIFIQIARAIEDEILSNGIKEEEQVPSTTELSKLY
                     KINPATVLKGINILVDKNILYKKRGIGMFVSDGAKTIIKEARKENFKHNFVKNLLQEA
                     NKLEINREELVDIIINFKED"
     gene            complement(12684..13343)
                     /gene="deoC"
                     /locus_tag="ACGI59_002878"
     CDS             complement(12684..13343)
                     /gene="deoC"
                     /locus_tag="ACGI59_002878"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFG9740657.1"
                     /translation="MKHILKTVDHTILKATTTWEDIKILCDEAVDMSVASVCIPPSYV
                     KRASEYLKGKIKICTVIGFPLGYQTTATKVFEAKDAIENGADEVDMVVNISDIKNKDY
                     DNIGKEIKEIKKAIGDKVLKVIIETCYLDEDEKIKMCEIVTMSGSDFIKTSTGMGTGG
                     ATLEDIKLMKEHVGKNVKIKAAGGVKSISDAEKFIEAGAERLGTSSICKILKNEDTTD
                     Y"
     gene            complement(13379..14566)
                     /gene="murJ"
                     /locus_tag="ACGI59_002879"
     CDS             complement(13379..14566)
                     /gene="murJ"
                     /locus_tag="ACGI59_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HFG9740658.1"
                     /translation="MKQEPCDFSHGRFRTGIIFIGITSVMSAFLQIKENFIVVGFGSI
                     PYNIVIIISIMLSTIFGPYILPIGAVVAMVVQLLFYMFFVKKTNYKYLYYLNFKDDSL
                     IKLLALLSPVFIGVAVNQVNSLVDTTLASTLVKGSIPALTYADRLNGFVTGTFTASIV
                     SVMYPMLSKLSAENNQKKFTSSVKSSINMIIISMIPISVASIFFATPVVRIIFERGAF
                     DARATQMTATALIFYAVGMTAFGLRDILGKVFYSLQDTKTPMVNGIISVGVNIVLDLV
                     LIKPMAHGGLALATSSSSIACILLLFLNLKRKVGYFGQDKIIKATLKSVVASLIMGVL
                     SYFTYKFIFGILGVGTFNEFVSLAISVIVGGGIYTLLMIIFKVEEVDMILNIAKRKLH
                     LKK"
     gene            complement(14643..>15543)
                     /gene="tnpB"
                     /locus_tag="ACGI59_002880"
     CDS             complement(14643..>15543)
                     /gene="tnpB"
                     /locus_tag="ACGI59_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS200/IS605 family element RNA-guided
                     endonuclease TnpB"
                     /protein_id="HFG9740659.1"
                     /translation="DKFSLQNALKDLDNAYKKFFKEKAGFPKFKSKKINRFSYKTNFT
                     NGNIMYCGQHIKLPKLGMVKIRDKQVPKGRILNATISKEPSGRYYVSLCCIDVDIEVF
                     ENTNNHIGLDLGIKEFCISSCGEFIENPKYLKKSLNKLAKLQRELSRKTIGSLNRNKA
                     RLKVARLQEHIANQRNDFLQKLSTKLIKENDIICIEDLQVKNMIRNRKLSRLISDVSW
                     SEFIRQLEYKANWYGRQIVKVGKFFASSQICNKCGYKNEEVKNLNIREWICPSCNETH
                     DRDINASINILKEGLRLITIQNK"
BASE COUNT         5565 a         2269 c         1873 g         5836 t
ORIGIN      
        1 ttaatttcta aaaacatcat aatttttcta atagacttaa tcacttttaa tatatatagt
       61 aaaacaaaaa acttatattt tataaatatc ataatttttt tactgatatt ttattatata
      121 aattttatag aattaattgt caatttttta tgaaaatggt aagatattta tggatggtat
      181 cttgttataa gaactatact ttaaatgttt ttaattattt tgtcttatgt aactcatatc
      241 ctcaggaatg agattagaaa ttaagatgtt gtatcacaaa gtggaggtga tactattaaa
      301 ataggcttta ttggttctgg aaaagttgga acatctttgg caaattattt tttatcaaaa
      361 ggttattgtg tttcaggctt ttatggaagg aatcaattat ctttattaga atcaacaaat
      421 ttaacaaaaa caaagattta cagcaatttg aaagatataa tttatgaaaa cgatatatta
      481 tttattacaa catcagatga ttctattgaa attatagaca aaaaactttc caaatttaat
      541 ttaaaacata aatacatttg ccacacaagt ggctctttaa aatctagtat attgttctgt
      601 tcaaaaaaag caggtgcatt aatttattct atccatccta tatttgcatt ttccaataaa
      661 aatactgaca taaaaaaaat gaaagacatt tgtttttcca tagaaggtga taatgaacaa
      721 gatgatttag ttattcaaaa tttcatagat gatttgggaa accaattttt tataagagat
      781 aaggatactt catccactta tcatttagct aatgttttag tttcaaattt agtattatct
      841 ttactagata taggagtaag ttactttatt aaattaggtt tatctgaaga ggaatcttta
      901 aaagctatca gtcctttagt aaaaaaaaac atagaaaata tacttgataa tggctttact
      961 aaagctctta ctggtcctgt ttctcgtgga gatataacac caataaggaa acatctatct
     1021 gtattggata aaaaagatga ggatatttac aaaatactat ccttaaacct actaaaaata
     1081 attgccttag gaaatgacaa ttcattaaaa ggtataaaaa atattactat agaaaatgca
     1141 atcgaaaatt taataagtaa atctgaaaaa tacaaagaaa tttacaaact tttaggagga
     1201 aaaaacgatg aaaaatacca tacagacttt taagaatgct aaatatgaag gaaaaaaact
     1261 atctatgctt actgcttatg actactcaat tgcaaaaata atggatgagt gtgatataaa
     1321 tggtatttta ataggagatt cattaggaat ggtcataaaa ggtgaagaaa acactctatc
     1381 tgttaccata gacgaaatta tatatcatac aaaagctgta aaaaatggcg taaaaaatgc
     1441 cttaatcgta agtgatatgc cttttctatc ttaccatgtt tctatagaag atgcagttaa
     1501 aaacgctggt agacttataa aagaaggagg agctcatgct gtaaagttag aaggtggctc
     1561 aaacgttata aagcaaatag aaagcattgt aaatgctcaa atacctgtaa tggggcattt
     1621 aggattaact cctcaatcag taaattcatt tggtgggttt aaggttcaag gaaataccag
     1681 tgaaacagca agacaactta ttgaagatgc taaattaata gaaaaagcag gtgcattctc
     1741 tattgtcctt gaaggagtgc ctacgaaaat agctgagatg gtaactaatt ctatttcaat
     1801 tcctacaata ggtatagggg caggaattaa ctgtgatgga caaattttag tttatcaaga
     1861 tatgttggga atgtttggag attttgttcc taagtttgta aagcagtatg caaatatagg
     1921 agatataata aaagattcaa ttaaaaatta catcctagaa gttaatacag gtgcatttcc
     1981 acaagaaaaa cacagctttt caataaatga atctgagtta gaaaaattat actaatatta
     2041 aggatggtac aaaatgcttg taaaagaaat taaattacta agaaatataa tcaaagattg
     2101 gagaaaacat ggctattcta taggccttgt tacaacaatg ggatttttac atgaaggaca
     2161 ccaaagtctt ataaaaaaag ctgtaaaaga aaatgataaa gtagtagtga gtatatttgt
     2221 aaatccaact caatttggac caaatgaaga ttttaatagc tatccaagag atatagataa
     2281 agattttaaa tattgtatgg atagtggagc aacagtagtc tttaacccat caccagaaga
     2341 aatgtattta aaaggtaatt gtactaccat taacgtttct ggacttacag attttctttg
     2401 tggagctaaa agacctgttc attttggagg tgtctgccta gttgtttcaa agtttctaaa
     2461 tatagtaaca cctgataaag cttattttgg agagaaggat gcccaacaat tagctgtaat
     2521 taaaagaatg gtcaaggatt taaacataga tacagaaata ataggctgtc ctataattcg
     2581 tgaaaatgat ggattagcaa aaagttcgag aaatacatat ctctctgagg aagaacgaaa
     2641 atctgctcta atattaaata agagcttaag tttagcaaaa gaagaattag taaaaggcaa
     2701 tctaaatcca gagaatataa aagaattaat aacagctaaa ataaattctg aacacttagc
     2761 aaaaatagac tatgttgaaa ttgtagatag tgagacatta caacctgtta agcaaataga
     2821 acattctata cttgtggcaa ttgctgtgtt tataggaaaa accagactaa ttgataattt
     2881 tacatttgag ttaaatatat agtctataat tttatataat atatctaaaa aataatagaa
     2941 tacctaaaaa gtaataaatt acctaaaaaa taataaattg cttagaaaat aatagattac
     3001 aataactaaa attttaaaat taaattcctt aaaagtatga ttaattataa ttatataagt
     3061 atattcttat tacacttata ttaatttata ttaaatatct actaactcaa atattcataa
     3121 ttaaacttta aactggataa attttctttt aagaatgaaa gtttatccag ttttgcgatt
     3181 ataataacca gttttacata tccacttgta aaatatatct aagcaatata tcatactata
     3241 tagatttatg agtgaaataa tatctgctat ttactgaata tttttatttt tcgtttttta
     3301 gggggattag ttgtgtttaa agataagatt gatgaatgtg ttcatataat gactgcatat
     3361 attgctagtt taaaagaata ctattcattc atagaaactc aaattggtga ttttattaaa
     3421 aaatatggag aagatgttgt agagttatgt ctccatagag tgatgattct actatgcgaa
     3481 tgtggtttgg cataaaatta aatttattaa aatgggtaag aagaatccta tataatattc
     3541 ttcttaccca ttttaatata tcaatttcat agcttatcgt ttaaaatatt ttacatccat
     3601 tactagacaa ctttacattc tctttttaat tctagtatat ttaaatcatt ttagttgctc
     3661 cttatttatt cttattactt gttttattta taaaataatt ctttttaata atgttttttg
     3721 ttgatattcc tatcacacat ccaattattt atcattgtct tgaaattgtt tgcatactta
     3781 atttaatcca attgaaacac cccagcatag aatcccctgt aaaatagaag ttggattaaa
     3841 tcctaacatc tatacagaaa atactactcc aagcacaagc aatataatgg aatatattta
     3901 tcacttaatt ttttatactt tttaaatccc atacctaaaa cattaagtgc agctattaga
     3961 attaataatt gttgtggtat aaaattatgc ttgcttcata tttattcatt ttttgctata
     4021 aaagttcttg ttttacaaaa aacatttgtt ttgtatcaaa ataatactta taaattacat
     4081 aattctcata aaaatttcta gtattacttg tactcttaat aaataatagc caacaagtac
     4141 cttaaatcat tatatctata tctaataaga aacacagtaa gataatcttt tcgcccataa
     4201 cttaataaaa actgttgata ttagtagcaa ttgaatttag ttaatttatt tataatatac
     4261 ataaatttaa atattacgaa taaagtatta taaataggat actttctaag gggaggaaat
     4321 tatgatagat aatcaaaaat atgttatttt atcactagaa ttacatttat ttttttcaag
     4381 aattatgaaa gagcatgctc tttttttaga agcaggattc acaaataaaa attataatct
     4441 tgctatggaa gctgaccact ataaaaagca atttgaagat ttattatcat acactgttag
     4501 tgctagtaat ggtataatta gacctgatat attatattca gaagagcttg taactactct
     4561 cacatcagtt gcagaacaaa aaacagaaga gtttacaggg atagaaataa acaaaaacat
     4621 cactacaaga gaattaaatc tacaaagtgg tgtaaaccca caagttggtc aagacttagt
     4681 gaactatgta gctcaactta actctgatgc aataagatta cttgatgggc ttattaattt
     4741 taaagaaaga gtcttagatg gtgtactatc atgtactata tttacatcaa actaccctct
     4801 acttcttgaa catataatac atgaagcaaa tttatatcgt tcttatgtag ttgaccttga
     4861 aaataaaata gatattgagt caaaaaacgc taaagaaata gaattattct gggaccatat
     4921 tatgatggaa catgctctgt ttatgagagg attactagac ccctcagaag gtgaactaat
     4981 aaatacttca aatgattttg ctataaaatt taatgaatta attgaaaaaa caaacgaaat
     5041 gactgattct aatatcaaga acattacaga agaaactcta aatgaaactg ttgagtttaa
     5101 agattttaaa gaagcaggag catcaggaat agaacagtgt aagataaaat ctataatatt
     5161 accactttta gcagaccatg ttttaagaga ggcaaatcat tatattagaa tattggagag
     5221 ttataaaaac atgtaattaa aaattacttt taaaataatc tttgcatatt tagataaaat
     5281 aaaggtacag ttaactgtac ctttattttt tataaactta taaaatttaa acaagttatt
     5341 cataaaataa aataataatc atctataacc cacaacttta ttttatatta aataaaaagt
     5401 attagtggtt atgttttata agtatttctt tattcagtta tttattaaaa actaatagta
     5461 aatcttatac taatcgcata tttatgttat aactatattg taactatttt cgtaatctta
     5521 ggaaaagatt acttataaaa ttaaccttat acaataatat atcgtataat cttaattagc
     5581 ataatagtta caatattaaa atttaaatat gggagtgatt ttagtatgat aagacaaaca
     5641 ccattagaga aacgatatga caagctaggt cctcatattg taacagcact caaaaaacgt
     5701 tattttgatg cttattactg taaaactaga aatgaagctt tgcaacaaat tttagattta
     5761 attcctcaaa atcatgtagt atcatgggga ggatctgaaa cacttaaaga aatgggcgta
     5821 caaactgctg ttagagagaa aggatttaaa gttattgata gagatttagc aaaaactcca
     5881 gaagaaagaa ctgaaattat gcgtcaagca cttctatgcg atactttttt gatgagtagc
     5941 aatgcaatta gtgaagatgg acaactcttt aatattgatg gaaatggcaa tcgtgtagct
     6001 gcaatgatat ttggtccaaa aaatgttata gttgtagctg gtatgaataa aattgtaaaa
     6061 aacattgatg atgcaataca aagaactaga acaattgcat ctccagcagt tgttcagcgt
     6121 tttcaagatg tcaatacacc atgctatatt aatggaagtt gtatagattg cacaagtcct
     6181 gaaagcattt gtgcatacat ggttactaca agaatcagtc gtccagcaaa aaaaataaag
     6241 gtaatattgg taggagaaaa tcttggtcta taaaatatac atacatgtaa atagctctat
     6301 aaataataaa atcatatgaa ataacttatt tctaaaagtg gtagaataaa agcttctaca
     6361 agactttcat tctgccactt ttatattctt ttaatttgtg tttctatatt attaattgct
     6421 ccaattttca tatatattac taactatttt tattttcata atttttttgt tcttcatcaa
     6481 gctgtttata ataatcttta ttatagtcag ctagataatc tataccacct cctgtaatca
     6541 catttaataa tttcattgca gattcttgat taactttaca aacataatta tcaccagatt
     6601 taacaagctc taatgtaata ttaaattctt ttttctttgc agaatcaata tctttcatag
     6661 ttggatttgg catcatttct cctagtttag gaggattctt ttttaaaata gaaaccatat
     6721 cagtattgtt aatttttaaa tctacagtca ctttatcctt attatttttt atatctgaaa
     6781 tttcccaagt catattttta gaatacattt taaatccttc tttatcttcg tatataagat
     6841 tacctaaccc tgattttggg aaatacttat caacagtatc tgcatcttgt gtttttattg
     6901 aattaaacat actatccact tcatttttta tcttattatc attagtacaa ccagctaaca
     6961 ccagtactga tattgcaaac attataatac atagaataaa tttcttcatt aaattatatc
     7021 cccctatact tttttgcaca aatattattc tcttttttat cttttgaaaa agttgtagta
     7081 attgcatttt gtggacaaac ttttatacat tcaccacacc taatacagtc tggactattt
     7141 acatctttat atattggtat atcaagctta cattttgttt gacatgctgt acaatttgta
     7201 catttatctt tatcaatttt aaatctatat attgaaatag gattaaaaaa accatagatt
     7261 gctcctaaag ggcatatata cttgcaaaat ggcctataag taatgattga catcaataat
     7321 atcaataaca aaatacttaa cttccagcta aataaaaatc ctattgattc tcttaaattc
     7381 tcatttccta aaataagtgg tattcctccc aaaagtgttc ctgaagggca aatccattta
     7441 caaaaccaag gatttccacc ccctgctaca tttacaatag caactggcaa aataattaca
     7501 aatattatta agactatata ctttagatat tttagatatt tatcaccata aacttttttt
     7561 atctttactg gaaagggtac tttatgtagt aaatcttgta caagtccaaa gggacaaagc
     7621 caaccacaaa caaatctgcc aaaaaaagca ccaaatgcca ttaaaaaacc aacaacataa
     7681 aatgagaatt tatactctct gcttgataaa actgcttgta atgaacctat tggacatgct
     7741 ccaagtgcac ctggacatga ataacaattc aatccaggta cacataattt tttactttct
     7801 cctccatata tagttccttc taaaaatcca tttacatatc catttgttaa tgctgtaaat
     7861 gcaacttgag taataagtct tatattatta tttactttag ataataactt cattcctctt
     7921 ctcctaacca ataccaatac actcaagaca aatcataatt gctttagtta atactgtatt
     7981 tgtttctcca cgatatgtac ctaatgcaat aaatataata ctaattgcaa taatagaaat
     8041 gcctttttta ttatctttaa taaagttact cattggcttt taccattttt agtctttcat
     8101 ctataatttt tttccaatct tccttactat ttgcacctac aaccttctca cccacaaagt
     8161 ttcctttctt gtctacaaaa taagttgttg gaaactccat aactctttta aaatcagttt
     8221 ttttaagttc ttcagataca gtcaattgtt ggtaagttgc atctgacttt ttcataatgt
     8281 ctttaactaa atttttctct tcatttgaaa gaccagttct catatcagtt ttatcaactt
     8341 ctacaactaa tccctttata gctacattgg aattattact ctcatattct tttgataatg
     8401 cttcaagctc tggcatttca cccacacatg gtccacacca agtagtccag acattaataa
     8461 gagtaagttc tttatttgaa aatatctctt ttgttacctt atttccatca acatcctccg
     8521 tatctaaatt actaaaccat tcactattat tcttgttttt ttctactggc ttagattctt
     8581 ttttgctaca tccagaaatc atacctaaaa cacaaataca tcctaaaact actatgaaaa
     8641 accttttcat atctcacact cctagaattt atatagtata taaaatcgct gcttgaaata
     8701 aaaataactt atcttctata ttaaagtaat tatatttaat aaataagagc taatttaaaa
     8761 aaagatatac agttaaaatc tatttctttt cagacaaaat cttatatata tataatagct
     8821 tgtaaatctt aagattatat acatatattt ctaaagattt tcttaagaaa cagttagatt
     8881 ttactaatat aagtttatta attagcatta tactaattta tcttaatatg attttttgtt
     8941 gattttaagc tgtttatcgt ataaataaaa tttttcttga ctatatagtt actatatagc
     9001 ttattatata tagagagtaa taaaaggagg taaaatttat ggaaaattta tttacaagaa
     9061 aattcactac ttttgaattt ctaaaatttg tttctcctgc aattatatcc atgatattta
     9121 tatctttgta cacaataata gatggcatct ttgtatcgac attagttggt tctgatgctc
     9181 ttgctagtat aaatattgta ctacctataa ttaaccttgt ttgtggattt ggaataatga
     9241 tggcaactgg tggaggtgct atcgtttcta tacgtatggg tgaaaataga caggatgaag
     9301 ccaactctac attttctttt atagttttgt tttcattgat tgttgggatt ttattcacag
     9361 taatctcata tttctttatc aaagaaatat ctattttgct tggtgcaaca gataagttat
     9421 taccatattg tataacttat ggtaaagtta tgattttatg tacaccattt tatattttaa
     9481 aatttatatt tgagtacttt gcaagaactg atggaaattc taaatttagt ttatttctat
     9541 cagtcattgg tggtgtaaca aatataatat tggattatgt atttattaaa tattttggaa
     9601 tgggtctttt aggagctgca gttgcaactg ctataggtat tattttaact tgtgttttag
     9661 gtattattta cttcttatct aataaatcta cactaaaatt aagaaaacca aaaaccgatt
     9721 ttagacttat aagagatact atgataaatg gttcttctga aatggttaca gaattatcta
     9781 caggaattac aacattctta tttaatgtag tagctttaaa attagcagga gaaaatggac
     9841 ttgctgctct tactatagta ttgtatgctc attttttaat gacatcagtc tatctaggat
     9901 tcgctgctgg agtgtctcca ttaataagct ataattttgg tgctgaaaac agtgataaat
     9961 taaaagaaac atttaaacat tctctaaaat ttatatttgt ttcttctctt ttagtgttta
    10021 tttttgcttt agtatttgca ccatttattg ttagggtctt tgtaagtcca gataacacag
    10081 tatttaaact agccttacaa ggattaaaaa tatttgcatt tgcttttttg tttgttggta
    10141 taaatatatt tgcatcagga ttttttacag catttcacaa tggaaaaatt tcagctatta
    10201 tatcttttag tcgtgccttt gtttttataa tcataggaat cataattctt cctcctatgt
    10261 taaacatgac tggattatgg cttacagttc catttgctga ggttataacc atatttatat
    10321 ctattctatt tataaaaaaa tataaatgta gatataagta ttaaaaatta atacttaaaa
    10381 tcaacattta ttaaaactaa taaacctgct ttaaaataca aatttacaaa tttgattttt
    10441 aagcaggttt tattttagtt ataataagtc atttgcatac tctttaattg gtgcttttct
    10501 aagtaataaa attataaata ttatgcaaat aactataatt cctacttttg ttccaaataa
    10561 aataaaagga ttctgaaatg aataaataat tttttctaaa tacaatagca ttttatgcat
    10621 atttttagtt gcaaaattaa ctatataaat tatcattaat atcggtaatc ctatcaatat
    10681 agtaatacta gtaacttttc taactctata tgtaaaagct ccaagtaaca ctcctataaa
    10741 acctatacat atttcattta tacatatttg tacaaaataa tttaaataag ttagtggcat
    10801 acttgatgtt gaaggatcta tcagtgctga tatagatgtc caaatcatat ctctctttag
    10861 taaaactgat agttctatac tgtaacctgt tataatttct ataaataact tcgataaaaa
    10921 aatcaaaact atacctaaaa tagaaatcac aaagcttaaa ataattaaat ctatgacaat
    10981 agcttttata caactttttc tatctgctct tatacttaaa gctcctgaaa agtctttatt
    11041 tgctatattt actccataca caaatagcag tatagttata tatgttaaaa taggattcac
    11101 aatcgtggat aaattagttt tagatgcttc tccaaaaatc ttgagaataa acactggcaa
    11161 aatattcata acaattccca caatcataaa tataattata tacaatttag tatttttatt
    11221 aaaaaatttt atatatggct ttaattcatt caattacaat tcctccttct tagtcatgtt
    11281 aacaaacata tcttgtagcc ctatgtatcc tacatcaata tttgaatttt taagtatttt
    11341 ttcatcactt tcactaaaat caccatagta aaaataagca actgcatttc caaatgcctt
    11401 tttaggtttt atatttttta tacattctaa tttttctaac tcttctttat ttccagtaat
    11461 ataatgagct ttttgtttta tgacatcaat ttcttcatct ataataattt ttccctcatt
    11521 taatattatt acatgctcca gtaaatcatc tatttcatca atcaaatgag ttgatattat
    11581 tattgtcctt ggatttttta tataactatc taaaattatg ttataaaact cttgtctatt
    11641 tactgcatcc agaccaattg ttggctcatc aaatatggtg atagctgaat tagaacaaat
    11701 acctattata tttgaaagta tagttttcat acctcttgac agttgtttat actttttttt
    11761 agtatttaaa tcaaaatatt tgcataattc ttcttctaag cctttatcat aacttttata
    11821 aaaactagaa gaaaattcaa atatctgacc tactttaaaa tctggactat aaaattcttt
    11881 ttccctaacg atacaaactt cttccaaaac ttttatatct tcttttgggt tctttcctaa
    11941 tatttctatc tcaccttcat tttgaattaa ctgatttacc attatattta ataatgttgt
    12001 ttttccagct ccattcttac ctaataaacc atatatctta tttttttcaa agcttagaga
    12061 catattatta aaaatatttt gcttcttaaa actgtgagat aaattttcta tcttaataga
    12121 actactcata tattaatcct ccttgaaatt aattataata tcaaccaatt cttctctatt
    12181 tatttctaat ttgttagctt cttgaagtag gttttttaca aaattatgtt taaaattttc
    12241 ttttctagct tcttttataa tagtttttgc accatctgat acaaacatcc ctatccccct
    12301 cttcttatat aaaatatttt tatcaaccaa tatatttata ccttttaata ctgtagctgg
    12361 attaatttta taaagctttg aaagctctgt tgttgatgga acctgttctt cttcttttat
    12421 tccatttgat agtatctcat cttcaattgc tcgtgctatc tgaataaata taggttcttc
    12481 attatttaat actaatttca taggttcctc ctttctccat cagttagtta ctcaactaat
    12541 taactatata acttataata tcttgaaaaa tattttctgt caatactaaa taaaaaacag
    12601 agggaaaatt ccctctgttt tttgttcttt atatttataa ttaaattttg atttttgtaa
    12661 tcctatccca taagttaaaa actttaataa tctgttgtat cttcattttt taatatttta
    12721 catattgagc tagttcctaa cctttctgct cctgcctcta taaatttttc tgcatcagaa
    12781 attgatttaa ctccccctgc agcttttatt tttacatttt ttccaacatg ctctttcata
    12841 agttttatat cttctaaagt agctccacct gttcccattc cagtagatgt ttttataaaa
    12901 tctgaacctg acattgttac tatttcacac atttttattt tttcatcttc atctaaataa
    12961 catgtctcta taatgacttt taatacttta tctcctatag ctttttttat ttcttttatt
    13021 tcttttccta tattatcata atctttattt tttatatctg aaatgtttac aaccatatca
    13081 acttcatctg ctccattttc aatagcatct tttgcttcaa atacttttgt agcagtagtt
    13141 tgatatccca aagggaaccc tatcactgta catatcttta ttttaccttt tagatattct
    13201 gatgctcttt tcacatatga tggtggtatg caaactgatg ctacactcat atctacagct
    13261 tcatcacata aaattttaat atcttcccat gtagttgtag cttttaaaat agtatggtct
    13321 actgttttta atatatgttt cataaaatcc tcctaaattt ataattaata taattagatt
    13381 acttttttaa atgcaatttt ctctttgcaa tgtttagtat catatcaacc tcttcaactt
    13441 taaaaataat cattagtagt gtataaattc cacctccaac tataactgaa atagctagtg
    13501 atacaaactc attaaatgtt cctactccca aaattccaaa tataaattta tacgtaaagt
    13561 atgataaaac acccataatt aaagatgcaa ctaccgattt caaagttgct tttattattt
    13621 tatcttgtcc aaaatatcca acttttctct ttaggttcaa gaagagtaac aatatacatg
    13681 caattgatga tgaactagtt gcaagagcaa gaccaccatg agccattggt tttattaaaa
    13741 ctaagtccaa tactatattt acccctacag atataatccc atttaccata ggtgtttttg
    13801 tgtcttgtaa tgaataaaaa acctttccca aaatatctct taagccaaat gcagtcatac
    13861 caactgcata aaaaattaag gcagttgctg tcatttgggt tgccctcgca tcaaaagcac
    13921 ctctctcaaa aatgattcta acaacaggag ttgcaaaaaa aatagaagca actgaaatag
    13981 gaatcataga aattattatc atatttatac tggatttaac tgaactagta aacttctttt
    14041 gattattttc tgcacttaac ttagataaca taggatacat aacagatact attgaagctg
    14101 taaatgtccc tgtaacaaaa ccatttaatc tatctgcata agtaagtgct ggaatgcttc
    14161 ctttaactaa tgttgaggct aaagttgtat ctaccaatga gttcacttga ttaacagcaa
    14221 cacctataaa tactggtgaa agtaatgcta gtaatttaat aaggctatca tctttaaagt
    14281 ttaagtagta taagtatttg tagttcgttt ttttaacaaa aaacatgtaa aagagcagtt
    14341 gaactaccat agcaaccaca gcaccaattg gaagtatgta tggtccaaat atggtactta
    14401 acattattga aataattatt actatattat agggtatact tccaaatcca actacaataa
    14461 agttctcttt aatttgtaaa aatgctgaca taacactcgt aatacctatg aatattattc
    14521 ctgttctgaa cctcccatga ctaaagtcac agggttcctg cttcatagaa ttttgcatac
    14581 taaaatatgt cttactaatt ctccacaggc tatccccgta gttcctacgg ttcttacata
    14641 cgttatttat tttgaattgt tattagtctt aatccttctt ttaatatatt tatacttgca
    14701 tttatatctc tatcgtgagt ttcattacaa ctaggacaaa tccattctct tatatttaag
    14761 ttcttaactt cctcgttttt atacccacat ttattgcata tttgtgaact tgcaaagaat
    14821 ttacctacct ttacaatttg tctaccatac caattagcct tgtattctaa ttgacgaata
    14881 aactctgacc atgatacatc agaaattaag cgagatagtt tacgatttct aatcatattc
    14941 tttacttgta aatcttctat gcaaataata tcgttctctt ttattaattt agtagatagt
    15001 ttttgtagaa aatcatttct ttgattagct atatgttctt gaagtcttgc aacttttaat
    15061 cttgctttat ttctatttaa actaccgatt gtttttctcg ataattctct ttgtaattta
    15121 gcaagtttgt ttaatgactt cttaagatac ttaggatttt ctataaattc tccacaactt
    15181 gagatacaaa attctttaat tcccaaatct aaacctatat gattattagt attttcaaat
    15241 acttcaatat ctacatcaat acagcataat gatacataat atctaccact tggttcttta
    15301 gatatagtag cattaagtat tctcccttta ggtacttgtt tatcccttat cttaaccata
    15361 ccaagtttag gtagttttat atgttgacca caatacataa tgtttccatt tgtaaagtta
    15421 gttttatatg aaaatctatt aattttcttt gatttaaatt taggaaatcc tgctttttct
    15481 ttaaagaatt ttttatatgc attatctaaa tcttttaagg cgttttgtag tgaaaatttg
    15541 tca
//
LOCUS       DAYAPA010000104        15172 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.104, whole genome shotgun sequence.
ACCESSION   DAYAPA010000104 DAYAPA010000000
VERSION     DAYAPA010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15172
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.104"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            132..698
                     /locus_tag="ACGI59_002881"
     CDS             132..698
                     /locus_tag="ACGI59_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-class carbonic anhydrase"
                     /protein_id="HFG9740660.1"
                     /translation="MRKLEEILEYNKSFVENKEYEQYVTSKHPNKKIVVLSCMDTRLT
                     ELLPKAMNLKNGDVKLIKNAGATIMHPFGSIMRSVLVAIYEFDVDEVMVVGHHGCGMC
                     NVDTDKLLGKILDRGISNDVILTLRSAGIDTKQWLHGFDSAEESVKDSIDLIKGHPLI
                     PDGIIVHGLIISPETGKLDVVVNGYEDK"
     gene            1003..1464
                     /locus_tag="ACGI59_002882"
     CDS             1003..1464
                     /locus_tag="ACGI59_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFG9740661.1"
                     /translation="MNSNSEEKLNDVFLQLMRIYFIRTHFYFDKLGLYHGQPQILFSL
                     KMKDGQSQKELADKRKVKASTTTVMIKRMEKAGLVERKQDEKDQRVSRIFLTDKGLEI
                     CNKVEEFNKELEREFFLGFSEEEKIVLRRLLMQVRDNLKAGCHKHEDEKYF"
     gene            1532..3778
                     /locus_tag="ACGI59_002883"
     CDS             1532..3778
                     /locus_tag="ACGI59_002883"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9740662.1"
                     /translation="MFKLIKYLKKSALSIVIIVCLLVIQAVCDLSLPEYTSNIVNVGI
                     QQGGVENSVPSVIRESELNKITLFMDKSSKDKVLDNYTLLNKKDYVKYKDKYPGLKDE
                     SLYELNTKDKDTIDDLNVIFGKAILIVSGLEGDSKEVKAMKAQLMSKLPPQATQSGDV
                     DIFKLLSAMPKEQLDTLTKEMSKGFESMPESMITQSSVSYVRSEYEKIGIDTEKTQNN
                     YILFTGAKMLGIAMISMVATITVGFLAARVAASVGRNLRSGVFRKVMSFSNTEMNEFS
                     TASLITRSTNDIQQIQMLMVMLLRIVFYAPIMAIGGVIKVLNTNTSMAWIIAVAVVAI
                     LSLIVVLFSVVMPKFKLVQKLVDKLNLVSREIITGIPVIRAFSTEKHEEERFDKANKN
                     LTKTNLFVNRVMTCMMPAMMLIMNLITVLIVWRGAYSVDSGAMQVGDMMAFIQYTMQI
                     IMSFLMISMVSIMLPRASVSATRIDEVLVSDISIKDPEKSEAQEFKEDKKGLVEFKNV
                     SFMYPDAEEPILTNISFTAKPGETTAFIGSTGSGKSTLINLIPRFFDVTEGEILLGGV
                     NIKNVSQHDLREKIGYVPQKGVLFSGTIESNLKYGRKDATDEEMRTAAEIAQATEFID
                     SKPEAFKTEISQGGTNVSGGQKQRLSIARAIAKNPEVYIFDDSFSALDLKTDAALRKA
                     LKSKTAESTVLIVAQRISTILNAEQIIVLNEGEIVGIGTHKELLKNCEVYEQIALSQL
                     SKEELANE"
     gene            3771..5603
                     /locus_tag="ACGI59_002884"
     CDS             3771..5603
                     /locus_tag="ACGI59_002884"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9740663.1"
                     /translation="MSRMGRGPMGKSMGAGQKANDFKGTMRKLIAYLSKFKISIILVI
                     VFAIGSASFSIVGPKILGKATTKIFEGLVSKVSGGNVGIDFNAIGKILTFLLFLYLIS
                     ALFSFIQGFIMSGISQKVSYNLRKEISAKLDRLPMKYFDTKTHGEILSRITNDIDTLN
                     QSLNQSMTQLITSVTTMIGVLIMMLSISGIMTLVAVLILPISMFVISRIIKKSQKYFR
                     YQQEYLGNVNGQVEETYSGQTIVKAFNREDEVIEEFDKLNDSLYNSAWKSQFLSGIMQ
                     PLMMFIGNLGYVMVSILGGWLAIKKTIEVGDIQSFIQYVRNFTQPMTQIAQVANLLQS
                     TAAASERVFEFLEEEEEVQIVENAVSIDGLEGKIDFENVNFGYNPNKTIINDFSVNVK
                     PGQKVAIVGPTGAGKTTIVKLLMRFYDVNSGSILIDGHNIKDFNRSELREMFGMVLQD
                     TWLFSGSIMENIRYGKLNATDEEVIEAAKSAHVHRFIKTLPDGYKMKLNEEASNVSQG
                     QKQLLTIARAILADPKILILDEATSSVDTRTEVLIQKAMDNLMEGRTSFVIAHRLSTI
                     RDADMILVMNEGDIVEQGNHEELLKKGGFYANLYNSQFEEDEAM"
     gene            6328..7599
                     /gene="hflX"
                     /locus_tag="ACGI59_002885"
     CDS             6328..7599
                     /gene="hflX"
                     /locus_tag="ACGI59_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HFG9740664.1"
                     /translation="MAKKGITVGLNINNKSEDFNELMIELENLCSACDIDVVGSITQN
                     AKQVNRAFYIGTGKVEEILNLIKKENIEIVIFYNELSTSQLKNLEEKLNCEIIDRTAL
                     ILDIFAQRAKTREAKLQVEVASLKYMLPRLIGSNENLGRQSGGVGTKNRGSGEKKLEL
                     DRRRIEEKITSLNRELDDLKFQRETQRSMRRKSNLPNVALVGYTNAGKSSIMNKLVDI
                     FKNSEEKKVFEKNMLFATLETSVRNIVLANNKEFLLSDTVGFVSNLPHDLVKAFRSTL
                     EEACEADVLLHVIDISNPSYKSHIKVTEDTLKQIGADGIPMIHVYNKIDLIDVEVLDR
                     ILDSIDKEGIFVSVKKDINIDKMIKCICDSIFKDYVRCKFLIPYDKGHVVSYFNENTS
                     IINTEYREDGAILDVECSNIEYNKYKKYALE"
     gene            7832..8329
                     /locus_tag="ACGI59_002886"
     CDS             7832..8329
                     /locus_tag="ACGI59_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFG9740665.1"
                     /translation="MDAESLMKKYMVDDDSRKQAIFASIFVMQNKLQTTCDKLDPELT
                     MKQWLLLAIADSVDETLTLTKLGELMGCSRQNVKKLAIALEKKGFIQIEQNEKDSRAI
                     CLVTKDRVQEYSKKVRGMQEKVLQLLFEDFTEKEVEQLFFGIMKLSIGIENVKNYVEN
                     NDVGE"
     gene            8379..8903
                     /locus_tag="ACGI59_002887"
     CDS             8379..8903
                     /locus_tag="ACGI59_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HFG9740666.1"
                     /translation="MKNVVVIYKSKYGSTKKYAKWLSELLSCDIFETKNINIEKVMKY
                     DTIILGGGLYACNIAGISFLKKNYKELQKKNLVVFGVGAAPYDDEVIKKLREVNFKDE
                     LNKIPCFYCRGAWDETKMSFIDYTLCSFFKKSIAKKERQGLKPAEAPLLSGEAFDWCN
                     KENLTPILDYIREL"
     gene            9215..10579
                     /locus_tag="ACGI59_002888"
     CDS             9215..10579
                     /locus_tag="ACGI59_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HFG9740667.1"
                     /translation="MDIKELVKMQRKYYNTGKTRDISFRIEQLKKLKLVVSQNEEKIL
                     LALKKDLNKSDFEGFMTEVGMFYSELNFAIKNIRKWSKIKRVKSSMVNFPSISKVVPQ
                     PYGVTLIMSPWNYPFQLALIPLVWSLAAGNCVILKPSEYSVSTSSVVKDIVEDTFSKE
                     YVAVVQGSQEESEKLLLERFDYIFFTGSTNVGKIIMKSASEHLTPITLELGGKSPCII
                     LKDANIDLTAKRLTWGKLINAGQTCVAPDYVLVHEDRKNELIEKIKYYTNKYFGDNAC
                     NNEQFPKIINQRHFNRILSLIDKDKIVYGGNYNKETLKIEPTIVDNVNWDDNIMKEEI
                     FGPIFPILTYKDLDEVIQKIIQMPNPLALYIFTKNKYLENKLLEMIPAGGCCINDTVT
                     HIATNYLPFGGIGESGMGSYHGKAGFDTFTHYKSVLKKLNLDVPIRYAPYDNKLIKVL
                     KKIM"
     gene            10718..11884
                     /locus_tag="ACGI59_002889"
     CDS             10718..11884
                     /locus_tag="ACGI59_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFG9740668.1"
                     /translation="MKINWNTKYTTIAIYTFIIAASSIIFYLVSSQIDVFSNNLDAIF
                     TTLQPFIIGFAIAYLLNFILKFYEDRIFIKSEKLKKLKQSSKRGLGLLLTYATATLIL
                     YLFMHFVLPQVIESIVGLANDIPMYVNNATKLIDKLMTDLNLDEQYFNLAVDKWNEFV
                     TYIIKFVTDLIPILGSMLKNVASSIWNIVLGLIVSVYLLIDKEKFYGLSKKITYAVFT
                     EKQAARILELTHRSNYTFGRFLGGKILDSFIIGILTFVILTLVKMPYTLLISVIIGIT
                     NIIPFFGPLFGAIPSTLIVMFVSPIKAFWLLLIILIIQQIDGNIIGPKILGDSIGISA
                     FWILFSLLVAGKLLGFIGMVIGVPMFAVIYSIIKDIVESKLDKKGLPTDTSDYM"
     gene            12001..12783
                     /locus_tag="ACGI59_002890"
     CDS             12001..12783
                     /locus_tag="ACGI59_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFG9740669.1"
                     /translation="MAFIECKFKSKYTGGETDIIVILPVANGKELFSGKEIYDFEGKF
                     KTLYLFHGLEDDQSSWIRYTNVEHYAKNKNIALVIPRVETSFYTDMAYGHQYFTFVSE
                     ELPKFVRAIFPLSDKREDNFVAGMSMGGYGALKLALSKPKEFSAVASFSGSPDMIHEL
                     ENQQIETNADILFHDFGRPEDARNSENDLLYILKNLKERNEDIPKIYQFCGDRDFLYK
                     NNQMFKKYADSLGVDVEYEECVGGHEWRLWNLWIKKFIDIIG"
     gene            complement(12902..13555)
                     /locus_tag="ACGI59_002891"
     CDS             complement(12902..13555)
                     /locus_tag="ACGI59_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFG9740670.1"
                     /translation="MSKSEKGKETKASIISAARELFFEQGYHKTTTRQIAERANINLG
                     LISYYFSSKSEIGAIIYEDIRNEMQSLIYNYHEEGTMMMFFLAMNADLCFLLKNEAYR
                     NFYLDIAYEEAILSYQRSVTEMVMKRYVNDKESIEHDELILSCIGILAMKPEIIRVYT
                     SHKYDISSQAVIKHFTKQFLINLGHSTDLCEEILEELSKYYIDIVDNFTPIITRVRT"
     gene            complement(13579..14943)
                     /locus_tag="ACGI59_002892"
     CDS             complement(13579..14943)
                     /locus_tag="ACGI59_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HFG9740671.1"
                     /translation="MLLLIVTCAIYIWIGFYFSKKVKTASDFLIAGRNLPLPILAATI
                     AATSFGGGCLVGGVQWGAERGLWVGMYSTIGAALACFINAFFAGKLRSLSCDITPADY
                     IETRYGHSVFLRAYQSLVTPISILAIMGSQLISFGSVSTAFGIPYDIAVIIGVIVIII
                     YTYTSGMWGVAVSAFIQLAICIVFLPIVAYISLKLVGDNPLLMLQSMVKEPFFPGNKS
                     ISDFMYTVLPLVLGSIFSYESFLRYQCADNAKNAVKSSVIAGFILLFLAFPIGIIGAI
                     GGSLFPNISSVQVLPQMISTTLPPLISVLFLAAILAAIMSTADSLMTSMSAIISRDIY
                     NKLLHPNIEFNDLKNTLKYSQIASALTAIIGAIIALKFDSLLELLFWPAPLCTGVIFA
                     PFVIGLCWKGATKKGAIYAVIVSVILALLNMIGILTVFDRILVPIIGGSITIFIVSKF
                     SKKD"
BASE COUNT         5883 a         1673 c         2564 g         5052 t
ORIGIN      
        1 atacaagatt tatagtagag taataatctt ataaaataat ttaataaata taaattaaat
       61 tatatgtgta tatattgtac tttaatagtg atataataat aaaaaaattt ttaaagaggg
      121 tgatattaca tatgagaaaa cttgaagaga tattagaata taataaatca tttgtagaga
      181 acaaagaata tgaacaatat gtgacatcaa aacatccaaa taaaaaaata gtagtcttat
      241 cttgtatgga tacaagatta actgagcttt tgcctaaggc tatgaatttg aaaaatggag
      301 atgtgaaact aataaaaaat gcaggagcta ctataatgca tccttttgga agtattatga
      361 gaagtgtttt agttgcaatt tacgaatttg atgtagatga ggttatggtt gtagggcatc
      421 atggatgtgg tatgtgtaat gttgatacgg ataagttact tggtaaaatt ttagatagag
      481 gaatatctaa tgatgtaata cttacacttc gtagtgctgg aattgataca aaacaatggt
      541 tacatggatt cgattctgca gaagaatctg taaaagatag tattgattta ataaaagggc
      601 atcctttaat tccagatggt attattgtac atggtctaat tatatcacct gaaactggga
      661 agttagacgt tgttgtaaat ggatatgaag ataagtagag tatcataatg ttatataatc
      721 aagatactgt tgaatatgat gttgtttcaa aatttatttt gggacaactt ttttatttga
      781 aaatatatga aaaggtttat tttaagcttt acaataaaaa atataagtag ctgactaatt
      841 aaaaatctta ctattattta ttaaaattat aactatatga tataaaaata tttaataaat
      901 gaaatttata tgaatttttt ataaatatat tgaaaaaatg catgcagtat agtaaaataa
      961 tgcttagccg actaagtaat ataaaaaata aggagggcaa gtatgaatag caattctgaa
     1021 gaaaaattaa atgatgtttt cttgcaatta atgcgaatat attttataag aactcatttt
     1081 tattttgata aattaggctt atatcatggt caaccccaaa tacttttttc tttaaagatg
     1141 aaagatgggc aaagtcaaaa agaattagca gataaaagga aagtaaaagc atctacaact
     1201 acagttatga taaagaggat ggaaaaagct ggacttgtgg aaagaaaaca agatgaaaag
     1261 gaccaaagag tatctaggat ttttttgaca gacaaaggtt tagaaatatg taataaagtt
     1321 gaagaattta acaaggaatt ggaaagagag ttttttttag gatttagtga agaagaaaaa
     1381 atagtactta gaagacttct aatgcaagta cgagataatt taaaagcagg atgtcataag
     1441 catgaagatg aaaaatattt ttaaagttta aaataatgtt tttcaattta atgttattag
     1501 actaattaat attacagaag ggaagaaatg tatgtttaaa ttaattaaat atttaaagaa
     1561 atcggcatta tcaatagtaa taatagtctg tctattggta attcaagcgg tatgtgacct
     1621 ttctttacca gaatacacat ccaatatagt aaatgtaggt atacaacaag gtggagtaga
     1681 aaactctgtt ccatctgtga ttagagagag tgaattaaat aaaattactt tatttatgga
     1741 taaaagtagt aaggataaag tactagataa ttatactttg ctgaataaaa aggattatgt
     1801 taagtataaa gataagtatc caggtctaaa agatgaatct ctttatgagt taaatactaa
     1861 agacaaagac actatagatg atttaaatgt tatttttggt aaagctattt taatagtatc
     1921 agggcttgag ggcgatagta aagaggtaaa agctatgaaa gctcagttaa tgagcaaact
     1981 tccaccacaa gctactcaaa gtggagatgt tgatatattt aaattgttat cagcaatgcc
     2041 aaaggaacag ttagatacat tgactaaaga gatgagcaaa ggctttgaaa gtatgccaga
     2101 gagtatgata acacaaagct ctgtttcata tgtaaggtca gaatatgaga aaataggtat
     2161 tgatacagaa aaaactcaaa ataattatat attatttact ggtgctaaaa tgcttggtat
     2221 agcaatgatt agtatggtag ctacaataac agtaggattt ttagcagcaa gagtagccgc
     2281 aagtgtaggt agaaatttaa gaagtggagt atttagaaaa gttatgtcat tttcaaatac
     2341 tgaaatgaat gaattttcta cagcttcatt aattactcgt agtacaaatg atattcaaca
     2401 gatacaaatg ttgatggtta tgttgcttag aatagtattc tatgcaccta taatggctat
     2461 aggtggagtg ataaaggttt taaacacaaa tacttcaatg gcctggatta tagcagtagc
     2521 agtagttgct atcttatcat taattgtagt gttattctca gtggttatgc ctaagtttaa
     2581 actggttcaa aagcttgttg ataagttaaa cttagtaagc cgtgaaataa ttactggtat
     2641 tccagttatc agagctttta gtactgaaaa acatgaagaa gaaagatttg ataaagcaaa
     2701 taaaaattta actaaaacaa atctttttgt aaatagagtt atgacatgta tgatgcctgc
     2761 tatgatgctt ataatgaacc ttatcacagt tcttatagtt tggagaggtg catacagtgt
     2821 tgattctgga gcaatgcaag ttggagatat gatggcattt atacaatata caatgcaaat
     2881 aataatgtca ttcttaatga tatcaatggt atctattatg cttccaagag catccgtttc
     2941 agctacacgt atagatgaag tattagtgtc agatataagc ataaaagacc cagaaaaatc
     3001 agaggcacaa gagtttaagg aagataaaaa gggtcttgtt gaatttaaaa atgtttcgtt
     3061 catgtatcca gacgcagaag aacctatatt aacaaatata tcttttacag caaagccagg
     3121 agaaacaact gcctttatag gaagtactgg tagtggtaag tcaactctta taaatttaat
     3181 tcctcgtttc tttgatgtta ctgaaggaga aatattactt ggtggagtta atataaaaaa
     3241 tgtttctcaa catgatttaa gggaaaaaat tggatatgta cctcaaaagg gagtattgtt
     3301 ttctggtact atagaatcca acttaaaata tggtagaaaa gacgcaacag atgaagaaat
     3361 gagaacagca gctgaaatag ctcaagctac agaatttata gattctaaac cggaagcttt
     3421 taaaactgaa atttctcaag gtggaacaaa tgtatctgga ggtcaaaaac aaagactttc
     3481 tattgcaaga gcaatagcaa aaaatccaga agtttatata tttgatgata gtttttcagc
     3541 acttgactta aaaactgatg cagctcttcg taaggcttta aaatcaaaaa ctgctgaaag
     3601 tacagttcta atagttgcac aaagaattag tactatctta aatgcagaac agataattgt
     3661 tttaaatgag ggcgaaatag ttggaatagg aacacataaa gagcttctaa aaaattgcga
     3721 agtttatgag caaatagctt tatcacaact ttcaaaggag gagctagcta atgagtagaa
     3781 tgggaagagg tcctatggga aaatctatgg gagcaggaca aaaagcaaat gactttaaag
     3841 ggactatgcg aaaactaatt gcatatttat ctaaatttaa aatatcaata attctagtaa
     3901 ttgtatttgc tattggtagt gcgtcgtttt ctatagtagg tccaaaaatt ttaggaaagg
     3961 caactacaaa gatatttgaa gggctagtga gtaaagtttc tggaggaaac gtaggtatag
     4021 attttaatgc aattggaaaa atcttaacct ttttactttt cctttatttg ataagtgcac
     4081 tgttctcttt tatacaagga tttataatga gtggaatttc tcaaaaagta tcctataatt
     4141 taagaaaaga gatttcagca aaattggata gattgccaat gaagtatttt gatactaaga
     4201 cgcatggtga aatattatcg agaattacga atgatataga tactttaaat caaagtttaa
     4261 atcaaagtat gacacagctt ataacatcag taacaactat gataggtgtt ttgataatga
     4321 tgttatctat aagtggaatt atgacattag tggcagtttt aatattacca atatcaatgt
     4381 ttgtaatttc aagaataata aagaaatcac aaaaatactt tagatatcaa caagaatatt
     4441 taggaaatgt aaatggtcaa gttgaggaaa cttacagtgg ccaaacaata gtaaaagcat
     4501 ttaatcgtga ggatgaagta attgaagagt ttgataaact aaatgatagc ctgtataatt
     4561 cagcgtggaa atctcaattt ttatcaggta taatgcaacc acttatgatg tttattggaa
     4621 acttgggata tgtaatggta tcaatcttag gaggttggct tgcaattaag aaaacaattg
     4681 aggttggtga catacagtca tttatacaat atgtaagaaa ctttacacag ccaatgactc
     4741 agattgcaca ggttgccaat ttattgcaat caacagcagc agcttctgaa agagtatttg
     4801 agtttttaga agaggaagaa gaagtccaaa ttgttgaaaa tgcagtttca atagatggtt
     4861 tagaaggtaa aattgatttt gaaaatgtca actttggata taacccaaac aagactataa
     4921 taaatgattt ctcagtaaat gttaaacctg gtcaaaaagt tgctattgtt ggacctacag
     4981 gagcaggtaa aactacaata gttaaactgt taatgagatt ttatgatgtt aatagcggtt
     5041 caatactgat tgatggtcat aatattaaag attttaatcg tagtgagctt agagaaatgt
     5101 ttggtatggt tcttcaagat acatggttat ttagtggttc aattatggaa aatataagat
     5161 atggtaagtt gaatgctaca gatgaagaag ttatagaggc tgctaaatct gcacatgttc
     5221 atagatttat taagacatta ccagatggat ataagatgaa attaaatgaa gaagcaagta
     5281 atgtttctca aggtcaaaaa caattattga ctatagctcg tgctatttta gctgatccta
     5341 aaattttaat attagatgaa gctacaagtt ctgtcgatac tagaacagaa gtattgattc
     5401 aaaaagctat ggataatctt atggaaggta gaacaagttt tgttatagcc cacagattat
     5461 caactatacg tgatgctgat atgattcttg taatgaatga aggagatata gttgaacaag
     5521 gaaatcacga ggaacttcta aagaaaggcg gattctatgc caatctttac aattctcaat
     5581 ttgaagaaga tgaagcaatg taacaaaaaa ctatcttagc atagacatgt aattaataag
     5641 ataaaataaa tgagggtgcc ttaaaatgaa ctttttaatt cataaggcac tcttttgtgt
     5701 atgaaataaa gtgtattttt atcattttaa caatgaaaaa gaggattctc tatattttga
     5761 gatagccaca taattttatg tctttacaaa gtgtttattt tattataata tgtagtaagg
     5821 aaaatgttgg aggtgattat caaaatgtat atttaatttt attctttaaa ttttattata
     5881 aatagaattt aattttaata gttgatgatt atattgaagt atatgttttt attactttta
     5941 ttaatcatgc aaataactac ttaaatttaa gaatagggtt aaatataaaa tgataattac
     6001 atataagtta attaaactaa tgtattttta atatatattt agaattttaa tccttcttat
     6061 gatataagta gcttgttttg tattacttag atttgtaagg agttttttta ttgaaaaaac
     6121 tgcataatat atttgggagg aggtgtaaga aagtgatttt ttatataaga ttgagaaaat
     6181 aatgcacgat tatggttatt ataatagtaa ataagaagtg tcatttaagg ataagataaa
     6241 aagcaaaatt cttgatgaat ttaattatag agccataatc tcgtgctata gattaaataa
     6301 aaaatagcag ataaggagaa tatggatatg gcgaaaaaag gaataacagt aggattaaac
     6361 ataaataata aatctgagga ctttaatgaa ctaatgattg aacttgaaaa cttatgttca
     6421 gcatgtgata ttgatgttgt agggagtata actcaaaatg caaagcaggt taatagagca
     6481 ttttacattg ggacaggtaa agttgaggaa atattaaact taataaagaa agaaaatata
     6541 gagatagtta tattttacaa tgaactttct acatcacaac taaaaaatct tgaagaaaag
     6601 cttaattgtg aaattataga tagaacggca ttgatacttg atatatttgc acaaagggca
     6661 aaaactagag aagctaaact tcaagtagaa gtagcaagtt taaaatatat gttaccaaga
     6721 cttataggtt caaatgaaaa tttaggacgt caaagtggtg gagttggtac taaaaataga
     6781 ggttctggtg agaaaaaatt agaacttgac cgtagaagaa tagaagaaaa gataacatct
     6841 ttaaataggg agttggatga tttaaaattc caaagagaaa ctcaaagaag tatgagaaga
     6901 aaatcaaatc ttccaaatgt agctctagtc gggtatacaa atgctggtaa atcttcaata
     6961 atgaataaac tagtagatat atttaaaaac tctgaagaaa agaaagtgtt tgagaaaaac
     7021 atgttatttg ctacacttga aacttctgtc agaaatatag ttcttgcaaa taacaaagaa
     7081 tttttattat ctgatactgt tggttttgta agtaatctac cacatgactt agtaaaagca
     7141 tttaggtcaa cattagaaga agcatgtgag gcagacgtac tgttacatgt tattgatatt
     7201 tcaaatccaa gctataaaag tcatattaaa gtgacagaag atactttaaa acaaataggt
     7261 gcagatggta tacctatgat acatgtgtat aataaaattg atttaataga tgtagaagta
     7321 ttggatagaa ttttagatag tatcgataaa gaaggtatat ttgtttcagt taaaaaagat
     7381 ataaatatag ataaaatgat aaaatgtatt tgtgatagca tatttaaaga ttatgtaaga
     7441 tgtaagttct taattccata tgataaaggt catgtagttt cttattttaa cgaaaatacg
     7501 agcattataa atacagaata cagagaagat ggagctattt tagatgtaga atgtagcaat
     7561 atagaatata ataagtataa aaagtatgct ttagagtaaa ttttgaaaaa cttattatag
     7621 cctaaaatat atttaattta taatttatta taaattgatt gtaaatatga ttttatgaaa
     7681 cggtatagcc ataatgctat gccgttttgc cgtttataat aaaaataaat ttattgacat
     7741 aatagtaact aattactata atataaagta attagttact atttaaaatt atcaaaaaat
     7801 taaaatctaa gataaagaga agtgtataat aatggatgca gaaagtttaa tgaaaaaata
     7861 tatggtagat gatgatagta gaaaacaagc tatatttgca agtatatttg taatgcaaaa
     7921 taaacttcaa actacatgtg ataaattaga cccagaatta acaatgaaac aatggttact
     7981 tttagctatt gcggactctg tagatgaaac tcttacatta accaaacttg gagaattaat
     8041 ggggtgctca agacaaaatg taaaaaaact agctattgct ttagaaaaga aaggttttat
     8101 acaaattgag cagaatgaaa aagattcaag agctatctgt cttgtgacaa aagatagagt
     8161 gcaagagtat tctaaaaaag taagaggtat gcaggaaaaa gtattacagc ttttatttga
     8221 agattttaca gagaaagaag tggaacagtt attttttgga attatgaaat taagtatagg
     8281 aattgaaaat gtgaaaaatt atgtagaaaa taatgacgtt ggagaataag caagtatcaa
     8341 atcaactttg tatgacatca gagaggggga taatttatat gaaaaatgta gtagtgattt
     8401 ataagagtaa gtatggttca acaaaaaaat atgctaaatg gctttcagaa ttattgtctt
     8461 gtgatatttt tgaaactaag aatattaata tagaaaaggt tatgaagtat gatacgatta
     8521 ttttaggagg aggtctttat gcttgtaata tagcgggaat ttctttctta aaaaaaaatt
     8581 ataaagaatt gcagaaaaaa aatttagtag tatttggcgt aggagctgct ccatatgatg
     8641 atgaagtaat aaagaaatta agagaagtaa attttaagga tgaattaaac aaaattcctt
     8701 gcttttattg tcgtggggca tgggatgaga caaaaatgtc ttttatagat tatactcttt
     8761 gtagtttttt taaaaaatct atagctaaaa aagaacgtca aggtcttaaa ccagcagaag
     8821 cacctttatt gtctggagaa gcctttgatt ggtgtaataa agaaaattta actccaatac
     8881 ttgattatat aagagaactt taagaggtat atatttgtaa ttatttatta tctaaagtta
     8941 gatttcatag aaacttacta gacattattt ataaaataat attattaaag atggataatt
     9001 taattgtttc aataaggtta taattttaaa aaatgtttat tttaaaataa actattttat
     9061 aagcttactt atacttaata aggggctttt tttattggaa gatttatgtt atattgagat
     9121 gaatttatta tgatataaaa aataatgtta ggataaattt aaatcttttt ctaaaaatgt
     9181 attagtataa ttcttaaaga aaggattttt aacaatggat ataaaagagt tagttaaaat
     9241 gcaaagaaaa tattataata caggtaaaac aagagatata tcgtttcgta tagaacagtt
     9301 gaagaaactt aagttagttg tatcacaaaa tgaagaaaaa atacttttag cactaaagaa
     9361 agatttaaat aaatcggatt ttgaaggttt tatgacagag gtaggcatgt tttatagtga
     9421 attaaacttt gctataaaga atataagaaa atggagcaaa ataaaaagag ttaaatcaag
     9481 tatggttaat tttccatcta tatccaaagt tgttccacaa ccttatggtg ttactctcat
     9541 aatgtcacct tggaattatc catttcaatt agcacttata ccattagttt ggtcattagc
     9601 agcagggaat tgtgttatat taaaaccttc tgaatattct gtgtcaacat ctagtgtagt
     9661 taaagacata gttgaagata ctttttcaaa agaatatgtt gcagttgttc aaggaagtca
     9721 agaagaaagt gaaaagttac tattggaaag atttgattat atatttttta caggaagtac
     9781 taatgttggt aaaataatca tgaaatcagc atcagagcat ttaactccta ttactttaga
     9841 acttggagga aaaagtcctt gtatcatttt aaaagatgct aatatagacc ttactgccaa
     9901 aagacttact tggggaaaac taataaatgc aggacaaacc tgcgtggctc cagattatgt
     9961 tttagtgcat gaagatagaa aaaatgagtt aatagagaaa ataaaatatt atactaataa
    10021 gtattttggg gataatgcat gtaataatga gcaatttcct aaaatcataa atcagagaca
    10081 ttttaatcgg atattatctc ttattgataa agataagata gtctatggtg gaaattataa
    10141 taaggagaca ttaaagatag agccaactat agtagacaat gttaattggg atgataatat
    10201 aatgaaggaa gaaatatttg ggcctatatt tcccatttta acatataaag atttagatga
    10261 agtaatacaa aaaattatac aaatgccaaa tccattggca ctttatattt ttacaaaaaa
    10321 taagtaccta gaaaataaac ttttagaaat gattccagca ggcggatgtt gtatcaatga
    10381 tacagttact catatagcta cgaattactt accttttggt ggtattggtg aaagtggtat
    10441 gggctcatat catggaaaag ctggattcga tacatttact cactataaga gtgtgttaaa
    10501 aaaattaaat ttggatgtcc ctattagata tgctccatac gataataaat taattaaggt
    10561 tcttaaaaag ataatgtagg ttatattgta gaataataaa tataaataaa attgagtatt
    10621 tgtattctga atatatttta tttttaattt ggtatatttg ttataaaagt ggtgattttg
    10681 tgataaaatt taattatata gaaataagga ggaaaaaatg aaaattaatt ggaatacaaa
    10741 atacacaaca atagcaattt atacatttat aatagctgct agtagtatta tattttatct
    10801 agtatcatca caaattgatg tattttctaa taatttagat gctatattta caacattaca
    10861 accttttata ataggatttg ctattgcata tcttcttaat tttattttaa agttttatga
    10921 agacagaatt tttataaaaa gtgagaaatt aaaaaaacta aaacaaagtt caaaaagagg
    10981 cttgggtctt ttattaacat atgcaacagc tacattgata ctttacttat ttatgcattt
    11041 cgtattgcca caagtaattg agagtattgt tggactagca aatgatatac ctatgtatgt
    11101 aaacaatgca actaagctta ttgataaatt aatgactgat ttaaatcttg atgagcaata
    11161 ctttaatttg gcagttgata agtggaatga atttgttaca tatattataa agtttgtaac
    11221 agatttaatc ccaatattag gaagtatgtt aaagaatgta gcctctagta tatggaatat
    11281 tgtactagga ttaattgttt cagtgtactt attaattgat aaagaaaaat tctatggatt
    11341 aagcaaaaaa ataacttatg cagtatttac agaaaaacaa gctgccagaa ttttagaatt
    11401 aactcataga agtaactata catttggaag atttttaggt ggcaaaatat tagattcatt
    11461 cattataggt attctaacat ttgtaatact tacgttagtt aaaatgccat atacattatt
    11521 aatatcagtt ataattggta ttacaaatat tatccctttc tttggacctt tatttggagc
    11581 tataccatct acgctaatag tgatgtttgt atctcctata aaagctttct ggttattgtt
    11641 gattatatta ataatacaac agatagatgg aaatattata ggacctaaaa tacttggaga
    11701 ttcaattggt atatctgcat tttggatttt gttctcctta ttggttgcag gaaaattatt
    11761 aggttttatt ggaatggtta taggagttcc aatgtttgca gtaatttatt ctataattaa
    11821 ggatattgtt gaaagtaaat tggacaaaaa gggattaccg acagatactt ccgattatat
    11881 gtagctaaaa aatattatgt ataattaaaa atatataaat taagcaatat ttaaagcact
    11941 gagaaagtta aactcttggt gctttttaga tataaaatta ttatagaaga ggtgtttttt
    12001 gtggcattta tagaatgtaa atttaaatct aaatatactg gaggagaaac tgatataatt
    12061 gtaattttgc ctgtagcaaa tggaaaagaa ctttttagtg gtaaagagat atatgatttt
    12121 gaaggtaaat ttaaaacatt atacttattt catggtttag aagatgacca aagctcatgg
    12181 attagatata ctaatgtaga gcattatgct aaaaataaaa atatagccct agtaatacca
    12241 agagtagaaa ctagttttta tactgatatg gcgtatggac accaatattt tacttttgta
    12301 agcgaggaat tacctaaatt tgttagagct atatttcctt tatcagataa aagggaagat
    12361 aactttgtag ctggtatgtc tatgggggga tatggagctt taaaattagc gctttcaaaa
    12421 ccaaaagagt tttcggcagt tgcaagtttt tctggttcgc ctgatatgat tcatgagtta
    12481 gaaaatcaac aaatagaaac caatgcagac attctatttc acgattttgg tagaccagaa
    12541 gacgctagaa atagtgagaa tgatttactt tacatactta agaaccttaa agaaagaaat
    12601 gaagatattc ctaaaattta tcaattttgt ggtgatagag attttttata taaaaataat
    12661 cagatgttta aaaaatatgc agattctctg ggtgtagatg ttgaatatga agagtgtgta
    12721 ggggggcatg aatggcgtct ttggaattta tggataaaga aatttataga tataattgga
    12781 tagtacactt atggtattaa caggtatact ataaaatatc tttaaatatg ataaagactg
    12841 aaatcctaaa atcaaattat gattttaaaa tttcagtctc taagaattta ataaaatttt
    12901 attaagttct tacacgagtt attattggag taaaattgtc aactatatct atatagtatt
    12961 tactcaattc ctctaaaatc tcttcgcata aatccgtact atgaccaaga ttaataagaa
    13021 actgttttgt aaaatgcttt attacagctt gagaagatat atcgtacttg tgtgaagtat
    13081 aaactctaat tatttcaggt ttcatagcaa gtataccaat acaagaaagt attaattcat
    13141 catgttctat agattcttta tcattaacat atcttttcat gaccatttcg gttacacttc
    13201 tttgatagct taaaatagct tcttcataag ctatatccaa ataaaaattt ctatatgctt
    13261 cgttttttag aaggaaacaa aggtcagcat tcatagcaag aaagaacatc atcatagtac
    13321 cttcttcatg gtagttatag attaaagact gcatttcatt tcttatatct tcatatatta
    13381 tagctcctat ttcgctttta cttgagaagt aatagcttat tagacctaaa tttatattag
    13441 ctctttctgc aatttgccta gttgttgtct tatgataacc ttgctcaaaa aataattctc
    13501 tagcagcact tataatacta gcttttgttt ctttgccctt ttctgatttt gacatattta
    13561 ctgtgtatac ctctcttttc aatctttttt tgaaaatttg ctaactataa atattgtaat
    13621 agaaccacct attataggaa ccaaaattct gtcaaataca gtaagaatac caatcatatt
    13681 taataatgcc aatataacac ttactataac agcatagata gctccttttt ttgtagcacc
    13741 tttccagcaa agaccaatta caaatggtgc aaatattaca cctgtacata atggagctgg
    13801 ccaaaacaat aattctaata aactatcaaa tttaagtgct atgattgcac ctattatagc
    13861 tgtcaaagct gatgcaattt gagaatactt tagagtattt tttaaatcat taaattctat
    13921 attaggatgt aataatttgt tataaatatc tcttgatata atagcactca ttgaggtcat
    13981 taatgaatcg gcagtagaca ttatggcagc caatatagca gctaagaata atacagatat
    14041 taaaggtggc aaagtagtcg atatcatttg aggtaaaacc tgaacagatg atatatttgg
    14101 aaataaacta ccaccaatag ctccaattat tcctataggg aaagcaagaa ataatagtat
    14161 aaatcctgca attacactac ttttaactgc atttttagca ttatcagcac attgatatct
    14221 taaaaaactt tcataagaaa atattgagcc taaaactaat ggtaaaactg tatacataaa
    14281 atcactaatc gatttatttc ctggaaagaa tggttctttt accattgatt gtaacataag
    14341 taaagggttg tcgccaacta attttaagct tatataagct actattggca aaaacactat
    14401 acaaatagca agttgaataa atgctgatac tgcaactccc cacattcctg atgtataagt
    14461 atatataatg ataactataa ctcctataat tacagctata tcataaggaa taccaaatgc
    14521 agtagataca gaaccaaaag aaatcaattg actacccata atagctagta tagatattgg
    14581 tgttacaaga ctttgataag ctcttaaaaa tacactatga ccatatctag tttcaatata
    14641 atcagcaggt gttatatcac aagataagct tcttaatttt cctgcgaaaa atgcatttat
    14701 aaagcaagct aaagctgcac ctatagtaga atacattcca acccaaagtc ctctttcagc
    14761 tccccattga actccaccaa ctaagcaccc tcctccaaaa gatgtagcag caatggttgc
    14821 agctagtata ggcaaaggca gatttctacc tgcgattagg aaatcggatg ctgttttaac
    14881 tttttttgaa aaataaaaac caatccatat gtatattgca cacgtaacaa ttaaaagtaa
    14941 catagtcttt tctcctttat aaaatatttt aataaaatat acaaaatata tttaaatata
    15001 ttttatataa aagtagtacg atatttgttt tattggtttt agattaatat agctataata
    15061 atttatgata ttagatggaa attacgtaaa aatagaaaat tgaaaattct gaaatttttg
    15121 catattgata tttatctcat agattctttt ttgtaattgt aacacgcttt ta
//
LOCUS       DAYAPA010000105        15014 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.105, whole genome shotgun sequence.
ACCESSION   DAYAPA010000105 DAYAPA010000000
VERSION     DAYAPA010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15014
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.105"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            184..318
                     /locus_tag="ACGI59_002893"
     CDS             184..318
                     /locus_tag="ACGI59_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9740672.1"
                     /translation="MDLINTIDKVQQAHKPIDEIIETIRDIVDIIKILEPVYNFKSNQ
                     "
     gene            823..1584
                     /locus_tag="ACGI59_002894"
     CDS             823..1584
                     /locus_tag="ACGI59_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896901.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HFG9740673.1"
                     /translation="MIGIYFSGTGNSRYCVEKFLQEYDITANSYSIEDNELLQHINNH
                     ENIIFSYPVQYSNVPKILKDFIINHSTLWNGKRVFIIATMALFSGDGSGVLARILKKY
                     GAITVGGLHVKMPDSIGDEKVLKHSLEYNRKLVIKAEKKVTKAAKKLKHGSPPKEGLG
                     FLSHIVGLFGQRLYFINKTNMYTDKLKINTEECIVCGKCVNLCPMKNLVIKNCMIVAN
                     GQCTMCYRCVNNCAKKAITLLGKKVVEQSNIKKYM"
     gene            1649..2431
                     /locus_tag="ACGI59_002895"
     CDS             1649..2431
                     /locus_tag="ACGI59_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HFG9740674.1"
                     /translation="MEYYIVDSFATKLFKGNPAGVCVLDRRIPLELMQKIAEENNLPE
                     TAFVVKCKGNYELRWFTPKAEIDLCGHATLAAAYVISNFIDVNVKRIDFFTQSGKLEV
                     TRNGNLYEMIFPEIMPIEIELSPQQANLIGCVPSAVYSSRDLILLLNSEQEVINYKPN
                     YAQLRKLTDWLGIIITAQGSNTDFVSRYFCPELDSEDPVTGSSHCNLIPYWSEKLGKH
                     KMVAAQLSNRGGIIQCEVLKDNTVKISGEAVLFMQGTIKIDI"
     gene            2698..3117
                     /locus_tag="ACGI59_002896"
     CDS             2698..3117
                     /locus_tag="ACGI59_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjdF family protein"
                     /protein_id="HFG9740675.1"
                     /translation="MDKISGKLTVLFEEPFWVGIFERQDGKKYEACRVVFGAEPKEVE
                     VYEFILERFFSLDFGSIKLEKNVTKDNIGYKRMQRKVKKEQEKETIGTKAQNALKLQY
                     EERKQDRKNLAKNRKEEEKERLFNLKQEKRKAKHKGH"
     gene            complement(3220..3486)
                     /locus_tag="ACGI59_002897"
     CDS             complement(3220..3486)
                     /locus_tag="ACGI59_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HFG9740676.1"
                     /translation="MKNFILIYFIVINSIAFFSMYIDKKRAIRNEWRIKEATLMSIAV
                     IGGSIGSIIGMYSFRHKTKHIKFTFGIPFILFLQFLLVYFYILK"
     gene            3713..4414
                     /locus_tag="ACGI59_002898"
     CDS             3713..4414
                     /locus_tag="ACGI59_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HFG9740677.1"
                     /translation="MKFIVEKEVFDKLENVCFGVVVAKGIDNTKEIERISNLLDISID
                     RVEDYFKDKKVKESEEIIPYREAFRSLGMNPNKFMSSIEAMTTRVAKNKKLPHINPIV
                     DLGNSISLKYLLPMGAHDMDFRNDDVYVRFSKKGDKFVPFGETDVELMEEGELIYSVG
                     DMVKTRRWIWRQGEEGKITNSSKNIFFPIDGFTDANLDKVMSAREELAKLLKEIFNCE
                     IKVGFVDKDNPEMEI"
     gene            4496..5104
                     /locus_tag="ACGI59_002899"
     CDS             4496..5104
                     /locus_tag="ACGI59_002899"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFG9740678.1"
                     /translation="MKKTIIKNVNPMIENKFIGLFEIEYKNKLDEDKVWMVASRKSSE
                     QLKNIYLKNEEDSVDAVVIVGLHKSSKKLILIRQFRVPINGYIYELPAGLVDEGESID
                     ISVERELREETGLTLLEIKKNKSIDKVYLSPGMSDESVAFVYCICDGDITDEFLEPDE
                     EIEALLISQEDAKEILQSNHKIDTKAFLILQMFVSLGAKLFE"
     gene            5428..5892
                     /locus_tag="ACGI59_002900"
     CDS             5428..5892
                     /locus_tag="ACGI59_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4624 family lipoprotein"
                     /protein_id="HFG9740679.1"
                     /translation="MKKLVIILISIISMVSLTACKKSNESKYNNLNNKESGQTTIEMD
                     LDKNYDTSDPFVNARLFCVSNDIDVLDTEISFKMDGDSGIVEIKDNKTYETLWSNTWH
                     GSVDNDTFTISLSNIQKEKEYAICFTGIKINHAVVKVTFESNLVKEKEKPSK"
     gene            6529..7545
                     /gene="gap"
                     /locus_tag="ACGI59_002901"
     CDS             6529..7545
                     /gene="gap"
                     /locus_tag="ACGI59_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFG9740680.1"
                     /translation="MKIKVGMNGFGRIGRAVLRIAQEELGDNIEIVAINARATTESLA
                     HLFTYDSCYGTFRGEVEAKDEDTLVVNSKEIKILRYNDPEELPWKELGVDIVIESTGL
                     FTQREKAEKHIKAGAKKVIITAPGKNEDITIVIGVNEKEYDNEKHNIISNASCTTNCL
                     APFAKVLDEKFGIVKGLMTTVHSYTNDQRILDKSHKDLRRARAAAESIIPTTTGAAKA
                     VSRVLPQLEGKLNGFSLRVPTPTVSLVDLVCELKENVTSEQVNSVLKDAAEGELKGVL
                     GYCDKPLVSIDYKGDSRSSIIDALSTMVIEDNMVKVVSWYDNEWGYSSRTVDLVKYVA
                     EKLK"
     gene            7940..9109
                     /locus_tag="ACGI59_002902"
     CDS             7940..9109
                     /locus_tag="ACGI59_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9740681.1"
                     /translation="MEKLTDRRQVKAVSMETFIFLILLVVGFGYVGSIMGAGMMFKVI
                     MSTAHALLLETVFLIMAMAVLAGALSALLSEFGVIALINKIFAVFMKPLYGLPGASIA
                     GAITTYLSDNPAIIPFAKDKTFTQYFKQYQVPALCNLGTAFGMGLILTTFMISQGTEY
                     VLPALIGNLGAIIGSVISVRIMLTFTKKFYKYSPEEDKATGTLEKKDEFREIREGNVF
                     QRALDAILEGGKMGVDMGMAIIPGVLVVCTLVMLLTFGPSTDPVTGQEVYTGAAYEGI
                     KLLPVIGDKLGFILEPLFGFTSPEAIAFPITSLGAVGAAMSLVPEFIKSGAITPNDIA
                     VFTAMGMCWSGYLSTHIGMMDALNARQLAGKAILSHTIGGLCAGAAAHFIFTLVG"
     gene            9489..13742
                     /locus_tag="ACGI59_002903"
     CDS             9489..13742
                     /locus_tag="ACGI59_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase-related protein"
                     /protein_id="HFG9740682.1"
                     /translation="MDELFHIGIDVGSTTVKVVVLNNSNNIVHKEYRRHYSDVKKSVK
                     EVLNGIYEKLGDINTTIIITGSGGIGISKKLGVKFVQEVISSTKSIEYFHPETDVVIE
                     LGGEDAKITYLSGGIDQRMNGICAGGTGAFIDQMASLLKTDASGLNELAKGYNVIYPI
                     ASRCGVFAKTDIQPLINDGANQTDIAMSIFNAVVVQTVSVLSCGRKIEGNVAFLGGPL
                     YFLSELREAFKRVLDLKDENIIFPENAQLYIAIGAGLLSVDEDNIQLKSLIEKLDSIK
                     NIEDGEVNLLEPLFKDKNEYEEFSRRHEKEKINYVDINSIKSNCYLGIDAGSTTTKAA
                     LIDEDGRLVYSYYNSNEGNPLKTTIKVINEVYDILPKNIKILSSTVTGYGEGLIKKAL
                     KIDNGEIETIAHYKAAKFFNKDVDFILDIGGQDMKCLKIKDGVIDNIILNEACSSGCG
                     SFLETFASSLSMTIEEFAHEGIYSQNPVDLGSRCTVFMNSRVKQAQKEGASVGDISAG
                     LSYSVIKNALFKVIKIRDLNEIGNNIVVQGGTFYNDLVLRSFEKLIGKNVIRPNISGI
                     MGAFGSALIAKEKYESGYKTSLLSKEELNNIKLEASVTRCKGCSNHCLLTINKFSDNE
                     IFISGNRCEKGEAIYGDKKIGLEKKKPINLFKYKYNRIFRYKPLEKEEAKNGEIGIPR
                     VLNMYEDYPFWFTFFNAMGFRVVLSDRSSKQLYETGITSIASETVCYPGKLVHGHIEN
                     LIAKGIKNIFYPSVTNENKEDSNADNYYNCPVVISYSEVIKNNVENIRNKNINYINPF
                     ISLNDKEKLKKRLYDELSRHFSDIKITKEEVNHAVDKATDEQNLFKMEIQAAGERALK
                     EIKERNMKGIVLCGRPYHIDPEINHGMPELINSLDMAVLTEDSICHLANVQRPLRVVD
                     QWVYHSRLYKAASFVRDKNYLELVQLNSFGCGLDAVTTDQVQEILNEKSKIYTIIKID
                     EGNNLGAAKIRMRSLKAAMFERESKNIDVKNIKVNRIQYAKNNQITNKHTILAPQMSP
                     IHFQFLEKAVNLSGYNIEILKDTDSSVIEEGLRYVNNDACYPAIIVVGQLITALKSGK
                     YDLNNTSVTITQTGGGCRATNYIGFLRKAIYDAGFKDVPVIALSVNGIEDSGIMDNIS
                     LKLINRLFMSVVYGDLLMKVLYRVRPYEKVTGSANALYEKWVDICKSSLVKAKISVFT
                     RNIKDIVKEFDNLEILDIKKPKVGLVGEILVKFHPIANNNLVDILEREGAEAVVPDLT
                     NFFLSCAFNTIYKHTHLEGSRKSRMIGEAFIYITGIYQRVYKKALDKSERFYAPANIK
                     NVAKSTEPVVSLGNQTGEGWLLTGEMVELLNEGVENIICMQPFGCLPNHIIGKGSIKE
                     LKRLYKNANIIPIDYDPSASEVNQLNRIKLMLSKAFKNI"
     gene            13891..14385
                     /locus_tag="ACGI59_002904"
     CDS             13891..14385
                     /locus_tag="ACGI59_002904"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFG9740683.1"
                     /translation="MSLVYDVYKSKLGNLYILSEDGFIVSIYIGDEKFNNLKNECIKK
                     GIDIKRSKEDLKEAISQIDDYFNGKRKVFNLNIRIEGTEFQKSVYREMLKIPYGETLC
                     YSDIARNINNPKSVRAIGQASKSNKIPIIIPCHRVVGKKDIGGYMGNHSDLKEILLNL
                     ERQS"
     gene            14501..14878
                     /locus_tag="ACGI59_002905"
     CDS             14501..14878
                     /locus_tag="ACGI59_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family (seleno)protein"
                     /protein_id="HFG9740684.1"
                     /translation="MCDKVIASKYHEKGFNCAESVIKAYNEEFNTDIPVCLGSGLGSG
                     CGVASLCGAVNASNIIIGYVKGRNHEDESTKAKVYAKDLTTTVRKEYGSELCIDLKKD
                     LVACREIMDFAYDSLKETLKKEL"
BASE COUNT         6109 a         1488 c         2664 g         4753 t
ORIGIN      
        1 tatagcaaaa aggctgttat attaatgtta gaggttcagg gaaaatatat aaagcaaaga
       61 tatttacaga taatgtagat tgcacagcta taagttagat aatagaatta tgtaatcaag
      121 aatttacaac aaagtataag ataaggataa tgctaaattg ccatacaggt gctagatgtg
      181 taaatggatt taattaatac tattgataaa gtgcaacaag cacataaacc aattgatgag
      241 attattgaaa ctataagaga tatagttgac ataattaaaa tattggaacc tgtttataat
      301 tttaaatcaa atcaataatg attaagatta aaataatatt aaggcatcaa tgttataaga
      361 gtacaatata aggagtacta tatagtactc cttacttata catattaggc aatagggaat
      421 ttttttatag ttaaattttc aaatattttt tagggaactt tataattgag aattaagggt
      481 atagtatgtg atgaacttac taaatttata attaggttat ataatggaat aatcatcaat
      541 agttttactt tagggaaaat atatgcttaa ggaagtatag cttactaata ttattaaaat
      601 gtatatttaa aatgataaaa aaagaattag acaagtattt agtaaattga ctatgcaaaa
      661 ttctatcaag caggaacact atgactttag tcatgggagg ttcagtaggt aacttgttga
      721 attaagttta tattatttaa ataaactact taatttagat aatataaaaa gtcagaattt
      781 taagaataga gctatataat cttagctatt aggagaagta ctatgatagg aatatatttt
      841 agtggaacag gaaattcaag atactgtgta gaaaaatttt tacaagaata tgatataaca
      901 gcaaattctt attctattga agataatgaa cttttacagc acatcaataa tcatgagaat
      961 attattttta gttatcctgt gcaatatagt aatgtgccaa aaatattgaa agattttata
     1021 ataaatcatt caactttgtg gaatggtaaa cgtgttttta ttattgcaac tatggctcta
     1081 tttagtggag atggctctgg tgtgttagct agaatattaa aaaaatacgg tgctataact
     1141 gttggtggat tacatgtgaa gatgccagat agtataggag atgaaaaggt attaaaacat
     1201 tctttagagt ataatagaaa gcttgtaata aaagcagaaa agaaagtaac taaagctgct
     1261 aaaaaactga aacatgggtc accaccaaaa gaagggttgg gttttttgag tcatattgta
     1321 gggttatttg gacaacgatt gtattttatc aataaaacaa atatgtatac agataaacta
     1381 aaaattaata ctgaggaatg tattgtatgt ggtaaatgcg ttaatttatg tccaatgaag
     1441 aatctagtaa ttaaaaattg tatgattgtt gcaaatgggc aatgtacaat gtgttatcgt
     1501 tgtgtaaata attgtgccaa aaaagcaatt acattgttag gtaaaaaagt tgttgaacag
     1561 agtaatatta aaaaatatat gtaagaaatt taaagtttta ttgaatgtaa attgatgtat
     1621 attttatgtt agaaagagag gttaaataat ggaatactac atagttgatt catttgcaac
     1681 taaactattt aaaggaaatc ctgctggagt ttgtgttctt gacagaagaa tacctttaga
     1741 gttaatgcag aaaatagcag aagaaaacaa tttaccagaa acagcatttg tagttaaatg
     1801 taaaggaaat tatgaattac gatggtttac tcctaaagca gaaattgatt tatgtggtca
     1861 tgcaacttta gcagcagctt atgtaatatc aaattttatt gatgttaatg taaaaaggat
     1921 agatttcttt actcaaagtg gaaagcttga agttacacga aatggaaatc tatatgaaat
     1981 gatatttcca gaaataatgc ctattgaaat agagttatca ccacaacaag ctaatttgat
     2041 aggctgtgta ccaagtgctg tttattcttc aagagattta atcttattgc taaacagtga
     2101 acaagaagtt ataaattata aacctaatta cgcacaattg cgtaaattga cagactggtt
     2161 aggtataata atcacagcac aaggaagtaa tacagatttt gtatcaagat atttttgtcc
     2221 agaattagat tcagaagacc ctgttactgg ttcatcacat tgtaatctta ttccatactg
     2281 gtctgaaaag ttgggaaaac acaaaatggt agctgctcaa ctttctaatc gtggtggtat
     2341 aattcagtgt gaagtgttga aggataatac tgtaaaaatt tctggtgaag cagtcttgtt
     2401 tatgcaggga acaataaaga ttgatattta aataagtata ataactaatt tattatgaaa
     2461 aataattaat tttcttagtg ttaacaataa taaagtagag ttaaaaagct tgatttcact
     2521 taaatcaata tgatagatgt tgatactaag attgacaagc atgattaaaa aagataatat
     2581 ttaatctata aagataaaaa cacagtctgg ttggtagtcc agacgcagca atagctgtca
     2641 gtaacctgcc tccttggttg tccgttcttt agaaagtaat ttaaggagga actagcaatg
     2701 gacaaaatta gtggaaaatt aacagtttta tttgaagaac cattttgggt aggtatattt
     2761 gaaagacaag atggtaaaaa atatgaagca tgtagggtag tttttggagc agaacctaaa
     2821 gaagtagaag tgtatgaatt tatacttgaa agatttttct cattagattt tggtagcatt
     2881 aaattagaaa aaaatgtgac caaagacaac attggataca aaagaatgca gagaaaagta
     2941 aaaaaggaac aagaaaaaga aactataggg actaaggctc aaaatgctct taagcttcaa
     3001 tatgaagaaa gaaaacagga tagaaaaaac ctagctaaaa atagaaaaga agaagaaaaa
     3061 gaaagactat ttaatttgaa acaagaaaag agaaaagcta aacataaggg tcattaattt
     3121 taattaaata tctttttaat aattgctaga aaaaggtgta taaaattttt aattttatac
     3181 accttaattt attgaaaaga ataaggtttt atatttcaat tactttaaaa tataaaaata
     3241 tactaataaa aattgtaaaa atagtataaa tggtatacca aatgtaaatt taatgtgttt
     3301 agttttatgt ctaaaagaat acattccaat tatagaacct atactacctc caattactgc
     3361 tatagacata agagtagctt cttttattct ccattcgttt ctaattgctc ttttcttatc
     3421 tatgtacatt gaaaagaaag caatagaatt aatcactata aaatatatta gaataaaatt
     3481 tttcacagtc ttgcctccat aaaaatatat tatacattat aaaacaagat atttgttttg
     3541 gaaagttaaa tttgtaatta gtacatataa aatatttgat aagaacaaat ataatattaa
     3601 ttggaaaatt taaaaaaatt aaacaattag caaatagggg gtatatttat aagacacaaa
     3661 gtgataaaat ctatttagaa aataatgtaa aataacagga ggataaagca aaatgaaatt
     3721 tatagtagaa aaagaagttt ttgataaatt agaaaatgta tgctttggtg tagtagttgc
     3781 aaaaggaata gataatacta aagaaataga aagaataagc aaccttttag atataagtat
     3841 agatagagta gaagattact tcaaagataa aaaagtaaag gaatcagaag aaattatacc
     3901 atatagagag gcatttagaa gtctaggcat gaacccaaat aaatttatga gttcgataga
     3961 agcaatgaca acaagggttg ctaagaataa gaaacttcct catataaatc ctattgtaga
     4021 tttaggaaac tctatttctt taaaatattt acttccaatg ggtgctcatg atatggattt
     4081 tagaaatgat gatgtatatg ttagattttc taaaaaaggg gataaatttg taccatttgg
     4141 agaaactgat gttgaattaa tggaagaagg agaattaatt tattcagttg gagacatggt
     4201 aaaaactaga agatggatat ggagacaagg agaggaagga aaaattacta atagctcaaa
     4261 aaatatattt tttcctatag atggttttac tgatgctaat ttagataagg taatgagtgc
     4321 aagagaagaa ttagcaaaat tgttaaagga aatatttaat tgtgaaatta aagtaggttt
     4381 tgtagataaa gataatcctg aaatggaaat ataataagtt gttgaatact tgttaagata
     4441 ttatatttac ttaatgatgt taaaaaggta ttaaaaaaga aaggtcgtag taaatttgaa
     4501 aaaaactata ataaagaatg taaatccaat gattgaaaat aagtttatag gactttttga
     4561 aatagagtat aaaaacaaat tagatgaaga taaggtttgg atggtagcat caagaaaaag
     4621 tagtgagcaa ttaaaaaata tttatttgaa gaatgaagaa gatagtgtag atgcagttgt
     4681 aatagttggg cttcataaaa gtagtaaaaa attaatttta ataagacaat ttagagtacc
     4741 tattaatgga tatatatacg agttaccagc aggtcttgtt gatgaaggag agtctataga
     4801 tatttccgtt gaaagagaac ttagagaaga aacaggactt actcttttag aaattaaaaa
     4861 aaataaaagt attgataaag tttatttatc tcctggaatg agtgatgaat ctgttgcctt
     4921 tgtttattgt atatgtgatg gtgatataac agatgaattt ttagaaccag atgaagagat
     4981 tgaagcgctt ttaatttcgc aagaagatgc aaaagagatt ttacaaagta atcataagat
     5041 agacacaaaa gcatttttga ttttacaaat gtttgtgagt ttaggagcta aattatttga
     5101 atagatatat agaaaagagg ttatatagaa gagtaaaata ataaagttta ttttacttgt
     5161 ataaattata atctcttttt ttatacaata aagtataatt ttgtgtattt ttaaataagt
     5221 gacttgactc ttaaattttc ataatatgtt ataaaccaat ttaagatact acaataatta
     5281 aaattccaaa attatttaac atatttgcaa ttaaaatgag aaaaggtaaa ggttgaatga
     5341 ttgaaaaatt tgatgtaaaa tagtagctta gtttaatatt atagtttgta gggttaaaaa
     5401 ttagaaattg taagggagat aaattctatg aaaaaattag ttattatttt aattagtatc
     5461 attagtatgg ttagcttgac agcttgtaaa aaaagcaatg aaagtaaata taataattta
     5521 aacaacaaag aatccggaca aacaaccatt gaaatggact tagataaaaa ttatgatact
     5581 tcagacccat ttgttaatgc acgtttgttt tgtgtgtcta atgatataga tgttttagac
     5641 acagagattt cttttaaaat ggatggagat agtggtattg tggaaataaa ggacaataaa
     5701 acatatgaaa ctttatggag taatacatgg catggaagcg ttgataatga tacattcaca
     5761 atttctcttt ctaatataca aaaagagaaa gagtatgcta tatgctttac tggaattaaa
     5821 ataaatcatg cagttgtaaa ggttactttt gaaagcaatt tagttaagga gaaagaaaaa
     5881 ccctcaaaat aaattccaga ttgtctataa cttgatattc agtacataaa agcagttgat
     5941 atttatgatt gactgctttt tcttatcaaa aaggagagtt tgtgcatttt tacttgaaat
     6001 agtttatgca catcaaaagt tggtgagcat gatattggaa atggtaagca tgaaatcttg
     6061 ataagacaag gaaataaatt taagttttca attaagtata tttccataat ttagttaaca
     6121 ataagctaat tattatttat attttgatat ataaataata attgtagcaa atttcaaaaa
     6181 atcatatatt ttttgaacta aataaaaatt tatttttatt attttaaatt agaggagatt
     6241 cttgaagata tattattttt aaaaataagt atagtttaaa aattcaaaaa aaagatggca
     6301 aaatatgtcc caagggacat tgagtaaaat aaatgatttc agcttattta cactaaaaag
     6361 tacatactaa atgaggggta aaataaagaa aaatgatata ctaattgtct tttgataata
     6421 aaaaaacatg gaatttgatt atttaatcaa gaaaaagtat tgaacaaatg atttatttag
     6481 tgtacaataa tcatgtaaaa acatagtaat ggagggagac atttcataat gaaaattaag
     6541 gtaggaatga acggatttgg aagaatagga agagctgtat taagaatagc tcaagaagaa
     6601 ttaggagata atatagaaat tgtagccata aatgcaagag caacaacaga atctttggca
     6661 catttattta catatgattc atgctatgga acttttagag gtgaagtaga ggcaaaggat
     6721 gaagacactc tagtagtaaa ctctaaagag ataaaaatat taagatataa tgacccagaa
     6781 gaattaccat ggaaagaact gggggttgat atagtaatag aatcaacagg attatttaca
     6841 caaagagaaa aggcagaaaa gcatattaaa gcaggagcaa aaaaagtaat tataacagct
     6901 ccaggaaaaa atgaagatat aactatagtt atcggagtta atgagaaaga atatgataat
     6961 gaaaagcaca atataatttc aaatgcatct tgtactacaa attgtctagc tccttttgct
     7021 aaagtattgg acgaaaaatt tggtatagta aaagggttaa tgactacagt acattcatat
     7081 actaacgatc aaagaatatt agataaaagc cataaagatt tgagaagagc aagagcagcg
     7141 gctgaatcta taatacctac cactacagga gcagcaaaag ctgtgtcaag agtactacca
     7201 caattagaag gaaaattaaa tggtttttca cttagagtac caacaccaac agtttcactt
     7261 gttgatttag tgtgcgaact taaagaaaac gttacatctg agcaagttaa ctctgtgtta
     7321 aaagatgctg ctgaaggaga attaaaaggt gttttaggat attgtgacaa gccacttgtt
     7381 tctatagatt ataaaggaga ttcaagatca tctattatag acgctctatc cacaatggtt
     7441 atagaagaca atatggttaa ggtagtttct tggtatgata atgaatgggg atattcttca
     7501 agaactgttg acttagtaaa atatgtagct gaaaaattaa aataatatat atttcttaaa
     7561 gcaaatttta aaaaaatttt ttaaaaaaat tatataaaac taaaaaaaaa gattatgaaa
     7621 aatattgaag gaaaaatttt ttcataatct tttttctttc atgcaaaaag atacaagtta
     7681 caacttatat ttgcatatta ggtcgttaga gaagtatata tatatttagt aaggaatcat
     7741 actttttata aaatgaaaga taagatgtcg aaaacttgca taaatctgaa aaatttttat
     7801 tattaaaata ctgatttcca ttttaaaaat gataaaaaaa tgacaaataa agttgcaaat
     7861 agtaatactt ataagtataa ttaaaaaaga gaaaaggttt tgttaaactt tataacaaaa
     7921 aaagaaggga tgattcaaaa tggagaagtt aacagacagg agacaggtta aggcagtaag
     7981 catggaaacc ttcattttcc taatattact agtagttggt tttgggtatg ttggtagtat
     8041 tatgggagca ggaatgatgt tcaaagtaat aatgagtaca gcacatgctt tactattgga
     8101 gacagttttc ttaattatgg ctatggcagt tcttgcaggc gcattaagcg cactactttc
     8161 agagtttgga gtaatagcgt taattaataa aattttcgct gtatttatga agcctttata
     8221 tggactacct ggtgctagta tagcaggagc tataacaact tatttatcag ataatccagc
     8281 tattatacct tttgctaaag ataaaacatt cacacagtat tttaaacaat accaagttcc
     8341 agcattatgt aacttaggaa cagcctttgg tatgggactt atcttaacaa catttatgat
     8401 ttctcaaggt acagaatatg tacttccagc attaattggt aatttaggtg caattatagg
     8461 tagtgtcata agtgttagaa taatgttgac atttactaag aagttttata aatacagtcc
     8521 ggaagaagat aaagcaacag gaacattaga gaaaaaggat gaatttagag aaatcagaga
     8581 aggaaatgta tttcaaagag ctttagatgc aattcttgaa ggtggtaaaa tgggggtaga
     8641 tatgggaatg gctataattc ctggagtcct agtagtatgt acattggtga tgttattaac
     8701 ttttggacca tctacagacc ctgttacagg tcaagaagtt tatacaggag ctgcatatga
     8761 aggaataaaa ttacttccag ttattggaga taaacttgga tttatattag aaccattatt
     8821 tggatttaca tcaccagagg caatagcatt ccctataact tcattgggag ctgttggcgc
     8881 agcaatgtct ctagttccag agtttataaa gtcaggagct ataactccta atgatatagc
     8941 agtatttaca gctatgggta tgtgttggag tggatattta agtacacata ttggtatgat
     9001 ggatgcatta aatgctagac agttggcagg taaagctatt ctttctcata caataggtgg
     9061 tttatgtgca ggagcagctg ctcactttat atttacactc gtaggataaa tgtaatcagc
     9121 taagataaaa aataatatac aaaaaatggt tgttgatatg taataagaat ttacatatca
     9181 acaaccattt ttttctgact aaagataatt tataatacat aaacattaag tggatattag
     9241 agttaagtta tatttatgta aaaagtaaca aaaatctgta gaaatttatg acaaaattta
     9301 gtacactaac tatatgttct tatttaaaat ttaaggttag aattaggaat tacctagtag
     9361 agtaagcttt gattaataaa agaatcataa aataaaaata ttagtcataa agattataaa
     9421 taacttactt aaaataaagc catagaactt acaaaaatta aaaaagcata taaatatgga
     9481 ggggtacaat ggatgaatta tttcatatag gaattgatgt aggttctaca acggtgaagg
     9541 ttgtggtact taataatagt aataatatag ttcataaaga atatagaaga cattattctg
     9601 atgtaaaaaa atcagttaaa gaagtactaa atggtatata tgaaaaattg ggagatataa
     9661 atactaccat tataataact ggttcaggtg gaattggaat atcaaaaaaa ttaggtgtaa
     9721 aatttgttca ggaagttata tctagtacaa aatcaataga gtattttcat cctgagactg
     9781 atgttgtcat agaacttggg ggtgaagatg caaagataac atatctaagt ggaggaatag
     9841 accaaagaat gaatggaata tgtgcaggtg gcactggagc attcatagac cagatggcat
     9901 cactcttaaa gacagatgcc agtggattaa atgaacttgc taaagggtat aatgttatat
     9961 atcccatagc ttcaagatgt ggtgtttttg ctaaaacaga tattcaacct cttataaatg
    10021 atggagccaa tcagactgat atagcaatga gtattttcaa tgcagttgtc gtacaaactg
    10081 tgagtgtcct ttcttgtgga agaaaaatag aaggaaatgt tgcgtttttg ggtggtccat
    10141 tgtacttttt atctgaactt agagaagcat ttaaaagagt attagattta aaagatgaaa
    10201 acataatctt cccagaaaat gcacaacttt atattgctat tggagcagga ttattatcag
    10261 tagatgaaga taatatacaa ctaaaatctc taatagaaaa attggatagt ataaagaata
    10321 ttgaagatgg agaagtaaat ttattagaac ctttatttaa agataaaaat gagtatgaag
    10381 aattttcaag aagacatgaa aaagaaaaaa taaattatgt ggacataaat tcaataaaaa
    10441 gcaattgtta tttaggaatt gatgcaggct caactacaac taaagcagca cttattgatg
    10501 aggatggaag acttgtatat tcttattata atagcaatga aggaaatcca ttaaaaacaa
    10561 ccattaaagt tataaatgaa gtatatgata tattaccaaa aaatattaaa atattgagtt
    10621 caactgtaac tggatatgga gaaggtctta ttaaaaaagc tcttaaaatc gataatggag
    10681 agatagagac tatagcacat tacaaagctg ctaaattttt caacaaagat gtagatttta
    10741 tacttgatat tggtggtcaa gatatgaagt gcctaaaaat aaaagatgga gtaattgata
    10801 atataatatt aaatgaggct tgttcatcag gttgtggctc atttttagag acatttgcaa
    10861 gttctttatc tatgacaata gaagaatttg cgcatgaagg tatttattct caaaatcctg
    10921 tagatttagg ttcaagatgt actgtattta tgaattctag agtaaaacaa gctcaaaaag
    10981 aaggtgcaag tgttggagat atatcagcag ggctatctta ttcagtaata aaaaatgctt
    11041 tatttaaagt aataaagatt agagatttaa atgagattgg taataatatt gttgtacaag
    11101 gtggaacctt ttataatgat ttggtgctta gaagctttga aaaactaatt ggaaaaaatg
    11161 taattagacc taatatttca ggaattatgg gagcttttgg ttcagcactt atagctaaag
    11221 aaaaatatga aagtggatac aaaacatcct tgttatctaa ggaagagttg aataatatca
    11281 agctagaagc aagtgtaact agatgtaaag gttgttcaaa tcattgtctt cttacaataa
    11341 ataaattttc tgataatgaa atttttattt caggtaatag atgtgagaag ggtgaagcta
    11401 tttatggtga taaaaaaata ggattagaaa agaaaaaacc aataaattta tttaagtaca
    11461 aatacaatag aatttttaga tacaaaccat tggaaaaaga agaagcaaaa aatggggaaa
    11521 tagggatacc aagagtatta aatatgtatg aagattatcc attttggttt acttttttca
    11581 atgctatggg ctttagagtt gtactttcag ataggtcatc aaaacagtta tatgaaactg
    11641 gtataactag tattgcatct gaaacagttt gttatccagg aaaattagtt catggacata
    11701 tagaaaattt aattgcaaaa ggtataaaaa atatatttta tccaagtgta acaaatgaaa
    11761 ataaagagga ttcaaatgca gataattatt ataattgtcc tgttgtaata tcatattctg
    11821 aagtaataaa aaacaatgta gagaatataa gaaataagaa cataaattat ataaatccat
    11881 ttataagtct aaatgataag gaaaaattga agaaaagact ctatgatgaa ttatcaagac
    11941 atttttctga tataaaaata actaaagaag aagttaatca tgcagttgat aaagctactg
    12001 atgaacagaa tttatttaaa atggagatac aagcagcagg tgaaagagca cttaaagaaa
    12061 taaaagaaag aaatatgaag gggattgtgt tatgtggaag accatatcat atagacccag
    12121 aaataaatca tggaatgcca gagcttataa attcattaga tatggcagtg cttactgaag
    12181 actctatatg tcatttagcc aatgtacaaa gaccacttag agttgtagac caatgggtat
    12241 atcattcaag attgtataaa gcagctagtt ttgtaagaga taaaaactat ttggaacttg
    12301 ttcaacttaa ttcatttggc tgtggattag atgcagtaac tacagaccag gttcaagaaa
    12361 tacttaatga aaaatcgaag atatatacta ttataaagat tgacgaaggc aacaatttag
    12421 gagcagctaa gataaggatg aggtccttaa aagcagctat gtttgagaga gaaagcaaaa
    12481 atatagatgt aaaaaatata aaggtaaaca ggatacagta tgctaaaaat aatcagataa
    12541 cgaacaaaca tactatatta gcaccacaaa tgtctccaat acatttccaa ttccttgaaa
    12601 aagctgttaa cttaagtggt tataatatag aaattctaaa agatactgat agtagtgtaa
    12661 ttgaagaagg gttaagatat gtaaataatg atgcatgtta tccagctata atagttgtag
    12721 gtcagctaat aacagcatta aaatctggca aatatgattt aaataacact tctgttacta
    12781 taactcagac tggtggaggt tgtagagcta caaattatat tggattctta agaaaggcta
    12841 tatacgatgc tggatttaaa gatgttcctg tcattgcact tagtgtaaat ggaatagaag
    12901 atagtggtat aatggataat atatcactaa aattaatcaa tagactcttt atgtctgttg
    12961 tttatggtga tttgctgatg aaagtactat atagagttag accatatgaa aaagttactg
    13021 gaagtgcaaa tgctctatat gaaaaatggg ttgatatatg caaatcttct cttgttaaag
    13081 caaaaatttc tgtatttact agaaatataa aagatatagt aaaagaattt gataatttag
    13141 agatactaga tatcaaaaaa ccaaaagtag gtttggttgg agaaatattg gtgaaattcc
    13201 atccaattgc aaataataat ttagtagata tattagagcg agaaggtgct gaagcagtcg
    13261 tgccagacct tacaaatttc ttcttaagtt gtgcctttaa tactatatac aaacacactc
    13321 atttagaagg aagtagaaag agtagaatga taggagaggc atttatatac ataactggaa
    13381 tatatcaaag agtatataaa aaagcacttg ataagagtga gagattttat gctccagcca
    13441 atataaaaaa tgttgctaaa agtacagaac cagtagtgtc attaggtaat caaactggag
    13501 aaggttggct tttaacaggt gaaatggttg aattattaaa tgaaggtgta gaaaacatta
    13561 tttgtatgca accttttgga tgtttaccta atcacataat tggtaagggt tctataaaag
    13621 aattaaagag gttgtataaa aatgcaaata taatacctat tgattatgac ccttctgcaa
    13681 gtgaggtaaa tcaacttaac aggataaaac ttatgctttc taaggctttt aaaaatatat
    13741 aagatttata ttttgatatg ttaaaagtta atgaatttaa aaatatactc tatattaagt
    13801 atctataaaa aagagttatc ctatttttta gggtaactct ttattagtta aaaaattata
    13861 tgtatactga tattaaaggg gggaagtaat atgagtttag tatatgatgt ttataaatca
    13921 aaattaggaa atttgtacat attaagtgaa gatggtttta tagttagtat atacatagga
    13981 gatgaaaaat ttaataattt aaaaaatgaa tgtattaaaa aaggaattga tataaagaga
    14041 agtaaggaag atttaaagga agcaataagc cagatagatg attactttaa tggaaaaaga
    14101 aaagtcttta atttaaatat tcgtattgaa gggactgaat ttcaaaaatc agtttatagg
    14161 gaaatgttaa aaatacctta tggagaaaca ttgtgttatt cagatatagc aagaaatata
    14221 aacaatccaa aatcagtaag agcaatagga caagcaagta aaagtaataa aattccaata
    14281 ataatacctt gtcatagagt agttggcaaa aaagatattg gtggatatat ggggaatcat
    14341 tctgatttga aagagatact attaaattta gaaagacaga gttaacttag aaagaaatgt
    14401 ttttattgtt aatatttgat ataataatag taaaaagaag tattttttac aaaaacaatg
    14461 aagaatttat tttaatttac aaaaatgagg gggaatttta atgtgtgata aagttatagc
    14521 atcaaaatat catgagaagg gatttaattg tgcagaatca gttataaaag catataatga
    14581 ggaatttaat acagatatac cagtttgttt aggaagtgga ctgggttcag ggtgtggggt
    14641 tgctagttta tgtggtgcag taaatgcatc aaatataata attggatatg taaaaggcag
    14701 aaaccacgaa gatgaaagta ctaaagctaa agtatatgct aaagatttaa ctacaacagt
    14761 aagaaaagag tatggttcag agttatgtat agacctaaaa aaagatttag ttgcttgtag
    14821 agaaataatg gatttcgcat atgatagtct aaaggaaact ttaaaaaaag aattataaga
    14881 aaataagcta tcaaatataa attatttgat agcttatttt tatagccgat actaaataaa
    14941 tcaaatatat tttagaatta ttatttgtaa ataaacagca aataatttta ggctttttat
    15001 ttaatatagt tttt
//
LOCUS       DAYAPA010000106        15001 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.106, whole genome shotgun sequence.
ACCESSION   DAYAPA010000106 DAYAPA010000000
VERSION     DAYAPA010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15001
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.106"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            535..729
                     /locus_tag="ACGI59_002906"
     CDS             535..729
                     /locus_tag="ACGI59_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase"
                     /protein_id="HFG9740685.1"
                     /translation="MSVIVMCSRCGKEIDKYRNKYITYYEKNTNAEIKVCINCALDLL
                     EKTRENQELNIQEFQDTIIL"
     gene            complement(831..1340)
                     /gene="vanZ1"
                     /locus_tag="ACGI59_002907"
     CDS             complement(831..1340)
                     /gene="vanZ1"
                     /locus_tag="ACGI59_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ1"
                     /protein_id="HFG9740686.1"
                     /translation="MKSRKHNITKGLFIVYIIILTWIILFKLQFDISSLETMNLRSIN
                     LVPFAGSLIINNRVDISEIILNVVIFVPFGIYVCMLKEEWSFIKKVIPIFITSLAFET
                     LQYIFALGASDITDLIGNTLGGIIGIAVFMLLSKIFKNNTIKIINVLALIVTIIVVLF
                     LGLVIFANL"
     gene            complement(1660..2049)
                     /locus_tag="ACGI59_002908"
     CDS             complement(1660..2049)
                     /locus_tag="ACGI59_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HFG9740687.1"
                     /translation="MKINKLPHAELKLMKYIWGVDDVLASRDIIEVMKLKYDWKKSTT
                     LTFLKNLVDKGFLTTDKVDRCTHYTIAIKEKDYLKVETKSFFSFMHNNSFKSFISALH
                     DDEVLDSKSLDKLEEYFKNLKEEDIDD"
     gene            complement(2357..3100)
                     /locus_tag="ACGI59_002909"
     CDS             complement(2357..3100)
                     /locus_tag="ACGI59_002909"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9740688.1"
                     /translation="MNSRFKKSATSILSLLAMTILLPASVNASEVENSSMKVTYSEKQ
                     VTDMNKLYDMAKNGITDVKSPNEKGIIKNERTGESMYVDAISTTQLLEVKQSKDLTSK
                     TYSKTVFLDTDTIKPLRKASISEDGWDKTGGVKASGTVTYTISKGDGAFQYISVSKVS
                     GNWSIADGQIRVTNRKVYINQTGFVSNGLGIKQQQRKDPTSNSFSYNAPSSWKPVGRD
                     SLQYNCGFESIATLVRGGSSWSFNFIVQP"
     gene            3248..3631
                     /locus_tag="ACGI59_002910"
     CDS             3248..3631
                     /locus_tag="ACGI59_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9740689.1"
                     /translation="MRKKLFLGIFIVLVIAAIVVLNIKNDKNIFIGESEHWTAKFVTT
                     EEQSELEIKYKDSKDVVKQKFNYSYEFIDGEDAIIILKDAVLESGQEKIIKRQRPGLY
                     SNRESNYKVIISWNKNEETINLKKK"
     gene            3966..4454
                     /locus_tag="ACGI59_002911"
     CDS             3966..4454
                     /locus_tag="ACGI59_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9740690.1"
                     /translation="MSLKYWFKNIKKKKQIKKDIKYFKGNKESVINYQEYYTKIYNHY
                     NEIKNINTIRKIEIEQTRIKGQLEKNNNPGNALFFSLIAVVFSLLANLFFSSLNIKDN
                     YINLILQSSSIIVLIFIAINTSLDIAVPYRDEIQFYTTALDVLEDMKKKKLKERKYKK
                     RR"
     gene            complement(4527..5285)
                     /locus_tag="ACGI59_002912"
     CDS             complement(4527..5285)
                     /locus_tag="ACGI59_002912"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9740691.1"
                     /translation="MIKINRTRKPIELTEEVQKQLTEEFKNNKEKSVWRKKYITNALL
                     EMSHGKCCYCEMKLIEEGKSLNVEHFHHKDKYPDEVVKWENLLPSCGRCNSEKGTHDT
                     KLEPIINPAINNPKDYLYLYNYRYKSKNRNKLGSDTIDILYLNDTDKLIKPRVNICMA
                     LFKRLESIKTFVEDYQNGTGTHTRRKNKIINEVKGVLRCAQPTEEYSAIVANVIVNDD
                     DYKYIKEIMSEFNLWDNELEELESKAKEIMLDSE"
     gene            complement(5282..6544)
                     /locus_tag="ACGI59_002913"
     CDS             complement(5282..6544)
                     /locus_tag="ACGI59_002913"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFG9740692.1"
                     /translation="MRKLDNIKKGAIFLDIILDSTNGRDAMKIENIEIKGIGGIKELS
                     LRFNKGLNIICGANGIGKTTILESIAHNFSYANASILKRNKKYDSGTVKMKVVLADEG
                     GISTWFDDEYKIHEFRASKSEMTGHHTYYSKYLLPFKTYRQMEYNQLNSISADKELDG
                     HDLGEISIKGINQVDIKKWLINRFLFYQVENSISEEQKCNFELALKCFEILDGKITFS
                     KILADSLDIMVNTPQGEVYFEYLSSGYKSCFYILLGIIKEIEYRFKDPYIKVEDFEGV
                     ILIDELDLHLHPEWQVKIISALKIVVPNAQIIATTHSPNMIQTLSPNEIIPLIMDENG
                     NVCKKDLELGEYGLQGWTIEEILTDVMGMKTTSSELYLDTMRKFDRAMDKENIDEIKK
                     YYDILMKMLHPKSTLRTILKIQVAGIID"
     gene            complement(6795..7607)
                     /locus_tag="ACGI59_002914"
     CDS             complement(6795..7607)
                     /locus_tag="ACGI59_002914"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFG9740693.1"
                     /translation="MKICITVGHSILKSGACTSAGGVVNEYQYNKSLAPVLADTFRKE
                     GHKADVIICPEKQFKTKAEEKTYKIPRVNSGGYDLLIELHLNASDGQGKGSEVLYYSN
                     KGLEYATRICNKLGTVFRNRRAKLDKGLYILNSSNPTAVLIESFFCDNKEDYEKAKKL
                     GHEGIAKLIVEGVLNKNINNEGVKQMYKHTIVYDGEVDKIPATVVGWGYNDGKILICD
                     IKDYVPGQTQNLYVIGGGACEKIGSMTKEKFTMIKGNDRFDTLYKALDFIDR"
     gene            complement(7607..7864)
                     /locus_tag="ACGI59_002915"
     CDS             complement(7607..7864)
                     /locus_tag="ACGI59_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFG9740694.1"
                     /translation="MDNLISFIPEQLLILVAALSIIGKGCKKYKQLDNKYIPVVLLIL
                     GIGFSIWMLGLSPVAVLQGVICWGISIGINQTYKQLKEENK"
     gene            complement(7880..8110)
                     /locus_tag="ACGI59_002916"
     CDS             complement(7880..8110)
                     /locus_tag="ACGI59_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin XhlA family protein"
                     /protein_id="HFG9740695.1"
                     /translation="MNEELFEADLKRHETRINKHGEEIDELKIANIESKAELKALCEN
                     LNSLTSMLKWLIGTMITTLVGFFIFAVQRGIF"
     gene            complement(8142..8279)
                     /locus_tag="ACGI59_002917"
     CDS             complement(8142..8279)
                     /locus_tag="ACGI59_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFG9740696.1"
                     /translation="MNWYKIITDFYNNGNWTKEQVKTAVEKNKITASEYKEITGEDYI
                     A"
     gene            complement(8280..8579)
                     /locus_tag="ACGI59_002918"
     CDS             complement(8280..8579)
                     /locus_tag="ACGI59_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9740697.1"
                     /translation="MIFLGNLMDTEEQNIKYVCMIHYDSSLLSEEDLKKGTLIDRLPT
                     QKYVENKEAKLFINIDTKEVFYRYTDIKSSIEDKVNSTEQTIADLTFQLMSNGVI"
     gene            complement(8737..9810)
                     /locus_tag="ACGI59_002919"
     CDS             complement(8737..9810)
                     /locus_tag="ACGI59_002919"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9740698.1"
                     /translation="MTFKELVNKVRNLVLEAKNVTIEDTENNFTSDNVEGALKEVFQN
                     GVNAKNNVVTALNSKGAEITTNNTWEEIKNKVNNKEGRLDLRETILSNSSSYLVMNGA
                     IKYIEKYSGDLKALEYEEPFFYVIKGTHLIKINAIDESVTFDITLSNDNFSCICITQD
                     YLFISDNTKLYKINKTTGIEVQSIEGSYYKLCTYGEFIYGIYGDETSSILHKIRISDM
                     YKMLTKDMSINSIYDFERGKFVCNKNAIYATTEHANSSGYTDCYLTKINFDFTIAKDF
                     RIGGYLYEKNIKFLNDFVFVSDMSGKIEISNGKISGLVKYDVNLNPIIYADSSRCTNF
                     DIYNGYIYMLYIQTLVALFKKRI"
     gene            complement(9841..10011)
                     /locus_tag="ACGI59_002920"
     CDS             complement(9841..10011)
                     /locus_tag="ACGI59_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9740699.1"
                     /translation="MYNLLKLMIEQKNYSTKEDLQHKIDVFYAVNRITEEQYLELTSL
                     LNKEETPAEPIK"
     gene            complement(10023..10370)
                     /locus_tag="ACGI59_002921"
     CDS             complement(10023..10370)
                     /locus_tag="ACGI59_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9740700.1"
                     /translation="MNRNNRIIYDQTGNIWLQTGEATGDIQEWSKITELNFLDVEFGS
                     IDYSKQYIESINPVTKEPIIKDIEVILTDEQKRLQALEKELSMLKEENKNRDSEIVNT
                     AFEVENIKLNNNL"
     gene            complement(10391..12103)
                     /locus_tag="ACGI59_002922"
     CDS             complement(10391..12103)
                     /locus_tag="ACGI59_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine-rich protein"
                     /protein_id="HFG9740701.1"
                     /translation="MQTEWNFNYANYVQNVSLPPGRYKLECWGACGGAVDTSDWTDCA
                     KGGYSKGEIVFKKRTNLQICVGQSGYEKVSEGSSLTRSGFNGAGAAGKVTTGSFAYSK
                     YGGGATDIRLYHPSATWGNTESLLSRILVAGGGGGMKNNFASARSIGHGGGYVGVNGV
                     GRDRDFCGGGSQYQGGTSYDTEEYHGSLGKGGYGNIGIGGGGGWYGGAGSYSNECGGG
                     GSGYALNKDSYKAPGYIPTPEYYLENIVMTTGGNTTKADGYAKITLLQALPFLTVSSY
                     NSTQATFKADHTDPALLTKIEWFIDDVLKETITTNLTEEKTINYTLEDNALHTLKIVV
                     TDSNNATAEKVLSISKNIMPLPENVNLNDISTKLVEVNAGFKVGKTSIINTLALKNIE
                     ASLNNTLVELSEKIKTSFDSSDTSVQDLQNQVTQKNNTITQLETELSKRKRFITGTYT
                     FTKTDAENFNLSIYDKEGTSKTLTIPVNMGFSPSLIVLSGVTFSTTSKSYVYFDNVCN
                     SNFYNFGYNSDSTHSNPKAVGILNVSNVGYSSLVLTLYKLSMSEAVGIWAKEGATLTY
                     KIYI"
     gene            complement(12120..13121)
                     /locus_tag="ACGI59_002923"
     CDS             complement(12120..13121)
                     /locus_tag="ACGI59_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFG9740702.1"
                     /translation="MAEQKYYTLLTTIGKASIANATALGNKVDLVKLQLGDGAGTEYN
                     PTEEQTSLKNVVWEGSVNNVKIDEENPNWIVIETVIPGSVGGFMIREVGIFDSKEQLI
                     AVSKYPETYKPTADSGSVKDLVIKIILVVSNTSSVNLKVDPTVILATLKDIQDLDSKI
                     DTTKTELTSNIETTKTELNTRIDTENEKQNIKIDQLIAGGSNVASTQTITIDDWVEDA
                     ENGFKATVTHSLLTHRIVVNIIDATTKENIVPNFKIVDDNSIEIRSEVKVELNVYVIN
                     GNAETHFINATVDDNRVSEMTTYSSKKIEDRLVNIEEKVNGGLSNIATSVNELITYC"
     gene            complement(13123..13740)
                     /locus_tag="ACGI59_002924"
     CDS             complement(13123..13740)
                     /locus_tag="ACGI59_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative phage tail protein"
                     /protein_id="HFG9740703.1"
                     /translation="MKLIDKLPSFYNNDITSKIQEAYDIELSTLRETYDDTFDQFFVD
                     TATWGLDYWENILSIKNRFDLNIEDRRSNIKAKMRGKGTTTAEVVKAISEAYTKTDVD
                     VKVFSNLFSFTLSFITNDCSYNTILELDKKIEEIKPAHLEHKFEMILFNQNDMFAGST
                     MNTGETVTIYPYSPTDITSFGELTITTGNDRSMEKVTLYPRQEVV"
     gene            complement(13741..14787)
                     /locus_tag="ACGI59_002925"
     CDS             complement(13741..14787)
                     /locus_tag="ACGI59_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFG9740704.1"
                     /translation="MYSSQTYDVVKNRTLSNINLDIYKGEGSFLSDMVSPVNSELAKF
                     YIELSYLHKKAFIEDNFDDFLDKRVNEFGVYRKLGTEATGEVTFEGKVGTVIPNGTII
                     SYNELLFVVIKDIVISSEIEQNISPVQALEIGIRYNIPANTEFKLDEINGITRIYNNL
                     DFKGGTEIETDEELKERFYKIQKNQATSGNKAHYEAWALEVEGVYNAKIYPRWDGPGT
                     IKVLIFGENNQAVDTEVIERCKEHIEEEMPIGPALTVLTPSVLDISISASIKLEAGYT
                     LDFVKESFLESINSYLINVNKEIIYTKVSAILAGTEGLHDFSNLLLNNKTENIVFEED
                     KVPSVTTLEFSEVV"
     gene            complement(14780..>15001)
                     /locus_tag="ACGI59_002926"
     CDS             complement(14780..>15001)
                     /locus_tag="ACGI59_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HFG9740705.1"
                     /translation="IYSWGYGTELAELIGQKYSKGLTESEASRYIKEALLVNPYILDV
                     NVKNTKFTDDLLSVDIIINTIYGEVEVNV"
BASE COUNT         4828 a         2510 c         1628 g         6035 t
ORIGIN      
        1 cactactctt tatatgtaat tttctactat tatatattta tttactctat aaaataattc
       61 cctttcaaaa cacttttctt aaatcactca aactaaaata catataagat tatcattagt
      121 cagtaagtat atatttttct aatttctaaa aatacatcca aatcatcaaa cgtttttagc
      181 aattacttta ttattataca gtgtccaagt aaagctccta aataaattgt tattcatata
      241 gcaaacaaat acttggtatc aattttttaa tatctattta aatagtattt tttattttgt
      301 cattttatag atattaatgt gtatttaata ctgctaaaaa ataataaaat tttataatct
      361 attcaagaat atactttttt ctataaatat tttacccata acctttattt ttttattaaa
      421 atgcattata ataaaaagta aatctaaatt tgatacagtt ttaacaatca ttattttcag
      481 aaaagtgata ctattgaata tattaagaat aattagagat ggaggaacta agatatgagc
      541 gtaattgtaa tgtgtagtcg ttgtggaaaa gaaatagata aatatagaaa taaatatata
      601 acttattatg agaaaaatac caatgcagaa attaaagttt gtataaattg tgcgttagat
      661 ttattagaaa aaacaagaga aaatcaagaa ttgaacatac aagagtttca agatacaata
      721 attctttaat aatttaggaa agcacctgta taggtgcttt ttatgtgtaa ataattttat
      781 atgtaactgt atcttacgtt ctattttaag ctaaacatat atacttattt ctataagttt
      841 gcaaaaataa ctaatcctaa aaataataca acaataattg tcacaatcaa tgccaataca
      901 ttgataattt ttatggtatt attcttaaat attttcgata atagcataaa aacagctatt
      961 cctatgattc ctcctagtgt gtttcctatc aaatccgtta tatcacttgc acctagtgca
     1021 aatatatatt gaagtgtttc aaatgccaaa ctagttataa aaataggtat aacttttttt
     1081 ataaagctcc attcttcttt tagcatacac acatatatcc caaatggtac aaatataacc
     1141 acatttaata taatctctga tatatctact ctattattaa ttataagtga cccagcaaaa
     1201 ggtactaagt ttatgcttct aagattcatg gtttcaaggg atgagatatc aaattgcaac
     1261 ttgaatagta ttatccatgt taaaataatt atgtaaacta taaataatcc ttttgtaata
     1321 ttatgttttc tagatttcat ttattacctc cttactatat tattgtatta ttttattaat
     1381 acaataatat agtaaggtaa tttatctgtc aactatttct tctaaaatta tattcctttt
     1441 ataatttcaa tatctttatc atatttacat gatttaagct attgtagtat ttcatatatc
     1501 tttaaatttt aattggtatt attctctata ttttttacta tcaaacacta aggaaataat
     1561 cacttttctt tatataatgt tctaccatca cataaaacta acattaaatc caaacgctac
     1621 tctcaattgt ctcaaactaa aaagatactg atagctttat tagtcatcaa tatcttcttc
     1681 tttcaaattt ttaaagtatt cttctaattt gtccaagctc ttactatcta aaacttcatc
     1741 atcatgtagt gctgaaataa aactcttaaa tgaattgtta tgcataaaac taaaaaaact
     1801 ctttgtctca acttttaagt aatctttctc ttttatagct atagtataat gcgtacatct
     1861 atctacctta tcagtagtta aaaagccctt atctactaaa ttttttaaaa atgtcaatgt
     1921 tgtgctcttt ttccaatcat acttcaactt catgacttct atgatatctc ttgaggctaa
     1981 tacatcatct actccccaaa tatacttcat tagttttagt tctgcatgtg gtaatttatt
     2041 aatcttcatt agtttacacc tcttaccaat ttatgtataa ttaaatttta tactaataat
     2101 agtatgtaat caatcagatt ggaacaaata taatattcca atttattcca tataaaaaat
     2161 aagcactcct tataataaag agtgctttag tatatattca agcatttatc taatacaatt
     2221 atagcatgtc ttatgtttta gtatgataat tttcgttcgt tttattatta caacttatac
     2281 tatatagttt tattttttat tatatcttta tggtgtcaat atatctttaa ttttaataaa
     2341 attcacttaa aatgttttaa ggttgaacga tgaaattgaa tgaccaagag cttcctcctc
     2401 ttactaatgt tgcaattgat tcaaacccac aattgtattg aagagaatct ctacccactg
     2461 gtttccaact agaaggtgca ttatatgaaa aagaatttga tgttgggtct ttcctttgtt
     2521 gttgcttaat tcctaatcca ttagaaacaa atccagtttg gtttatgtac acctttctat
     2581 ttgttacacg aatctgacca tctgcaatag accagtttcc agaaacttta ctgacagaaa
     2641 tatattggaa tgctccatct ccttttgata ttgtatatgt aactgtacca gatgctttta
     2701 caccaccagt tttatcccat ccgtcttctg atatacttgc ttttctaagg ggctttattg
     2761 tatcagtatc taaaaaaact gttttagaat atgtcttaga tgttaaatct ttagattgtt
     2821 tgacttctaa taattgtgtt gttgatatag catcaacata catagattct cctgttcttt
     2881 catttttgat tatacctttt tcgttgggac ttttaacatc agtgattcca tttttagcca
     2941 tatcatataa cttgttcata tctgttactt gtttctctga atatgttact ttcatagatg
     3001 aattttctac ttcactagca tttacacttg ctggtaatag tattgtcatt gcaagtaaag
     3061 ataaaatact tgttgcagat tttttaaacc tagaattcat actaatccct ccttttttat
     3121 taattatacc acatattcta tcatgataga atataaatat caaaaaaatt cagaaaattc
     3181 ataataatga tgttagataa ctgttttcat gttataatac atataattat tcaaaggagg
     3241 gctcagtatg agaaaaaaac tttttctagg tatttttata gttttagtaa tcgctgcgat
     3301 agttgtactt aatataaaaa atgataaaaa tatctttatt ggagaatcag aacattggac
     3361 agctaaattt gttactactg aagaacaatc agaattagaa ataaaatata aagacagtaa
     3421 agatgtagtt aagcaaaaat ttaattattc atatgagttt atagatggtg aagatgcaat
     3481 tataatttta aaagatgcag ttttagaatc aggtcaagaa aaaataataa aaagacaaag
     3541 accaggtctt tattcaaata gggaatctaa ttataaagta attatatctt ggaataaaaa
     3601 tgaagaaact ataaacttga aaaagaaata atagcaatat tattaatttt aaaaattgat
     3661 tattgaaaga cttatataaa aaggagtgtt tagaaaaata aaacactcct ttttatataa
     3721 gtctttgaaa attatggatg aaatatatca aagctttatc tttataaata taaaacttat
     3781 aattttgtct ttacaaatta tctagcctta tccattgttg accacacatc atcagaataa
     3841 atctctgtaa atttttctct tgtatcctgg ctctattttt atttaaaatc gttcgacaaa
     3901 ttgctgatat atattattaa gtgtgttata aattaaagta tattatatgg atttatggag
     3961 gggttatgag tttgaaatat tggtttaaaa atataaaaaa gaaaaaacaa atcaagaagg
     4021 atataaaata ctttaaggga aataaagaaa gtgtaataaa ttatcaagaa tattacacaa
     4081 aaatttataa tcattacaat gaaattaaga atattaatac aattagaaag atagaaattg
     4141 agcaaactag aattaaaggt cagttagaga aaaataataa tcctggtaat gcattgtttt
     4201 tctcccttat agctgttgtt ttttcattac ttgctaattt atttttcagt tcattaaata
     4261 ttaaagataa ttatataaat ttaattctgc aatcctcatc tataatagtg ctcatattta
     4321 tagctattaa tacatctctt gatatagctg tgccttacag agatgaaata caattttata
     4381 ctactgcatt agatgtatta gaagatatga aaaagaaaaa acttaaagaa agaaagtata
     4441 aaaaaagaag atgataaaga aatttttatc accttctttt tttatacttt caaatttaat
     4501 tgtatctagt tattttaata tgtatttcat tctgaatcta acattatttc ttttgcttta
     4561 ctttctaatt cttctagttc attatcccac aagttaaatt ctgacattat ttcttttatg
     4621 tatttataat catcatcatt tactataaca tttgccacaa tagcactata ttcttcagtt
     4681 ggttgagcgc atcttaatac accttttact tcgtttataa ttttattttt tcttcttgta
     4741 tgtgttcctg taccattttg ataatcttca acaaatgttt ttatagactc taaccttttg
     4801 aataaagcca tgcaaatatt tactcttggt ttaattaatt tgtctgtatc attcaaatat
     4861 aatatatcaa ttgtatcact accaagttta tttctatttt tacttttata tctataatta
     4921 tataaatata aataatcttt tgggttattt attgcaggat ttattatagg ttctaattta
     4981 gtgtcatgag tacccttttc actattgcat ctcccacaag atggcaataa attttcccat
     5041 ttcactactt catcaggata tttgtctttg tggtgaaaat gctctacatt cagtgatttg
     5101 ccctcttcta tcaatttcat ttcacaataa caacattttc catgagacat ctcaagtaga
     5161 gcatttgtaa tatacttttt cctccacacg cttttttctt tattattttt aaattcttct
     5221 gttaattgtt tctgaacttc ttctgttaat tcaataggct ttcttgttct atttattttt
     5281 atcaatctat tattcctgcc acttgtattt tcagtattgt tctaagtgta ctctttggat
     5341 gtaacatttt catcaaaata tcatagtatt tcttgatttc atctatattc tctttatcca
     5401 tagctctatc aaactttctc attgtatcta aatatagctc agaagaagtt gttttcattc
     5461 ccataacatc tgttaatatt tcttcaatag tccatccttg caacccatat tcacccagtt
     5521 ctaaatcttt tttacataca ttaccatttt catccataat taatggtatg atttcatttg
     5581 gtgataatgt ttgtatcata ttaggactgt gtgttgttgc tataatttgt gcgtttggga
     5641 ctactatttt taaagcactt ataattttca cttgccattc tggatgtaaa tgtaaatcta
     5701 attcatcaat taatataaca ccctcaaaat cctctacttt aatatatggg tccttaaacc
     5761 tgtattctat ttcttttata atgcctaata aaatataaaa acatgatttg taaccagagg
     5821 ataagtattc aaaatatacc tccccttgag gcgtattaac cattatatct aaactatctg
     5881 caagaatctt tgaaaaagta attttcccat ccaaaatttc aaaacatttt aaagctaatt
     5941 caaaattaca tttctgttct tctgaaatag agttctcaac ttgataaaaa aggaatctat
     6001 taataagcca tttttttata tcaacttgat taattccctt tattgaaatt tctcctaaat
     6061 catgaccatc taattcttta tctgcactta tactatttaa ttgattatat tccatttgcc
     6121 tatacgtttt aaatggtaat aaatatttac tataataagt atgatgacca gtcatttcag
     6181 acttgcttgc tctaaactca tgaattttat attcatcatc aaaccaagta gaaattccac
     6241 cttcatcagc tagtactacc ttcattttaa cagtaccaga gtcatatttt ttatttcttt
     6301 ttaatattga tgcatttgca tatgaaaaat tatgggcaat tgattctaat attgttgttt
     6361 tacctatgcc atttgcacca catattatat taagaccttt attaaacctc agacttaatt
     6421 cttttattcc accaatacct ttaatttcaa tattttcaat tttcattgca tctctcccat
     6481 ttgtactatc aagaattata tctaaaaata tagcaccctt cttaatatta tcaagttttc
     6541 tcattctatt caatcccttc attctctgat ttattcgaca ataaaaaaga gacagctatc
     6601 ctaatgatta actatctctg attcttgtca tttatttgtt atgatatacc tgttgccata
     6661 tttattctaa agattattac taatattaat ataagtttaa ctgtcttaat tatatcgtat
     6721 atttatagta tatttgtatt aaaagtgtat ataaaatgta agcaaagtgt aaattgattt
     6781 tataaatttt taatctatct atcaataaaa tctaatgctt tgtaaagtgt atcaaatcta
     6841 tcattaccct ttatcatagt aaatttttct ttagtcatag aaccaatctt ctcacatgct
     6901 ccaccaccaa tgacataaag attttgcgtc tgacctggca cgtaatcttt tatatcacat
     6961 attaatattt taccatcatt atagccccaa ccaactacag ttgcagggat tttgtcaact
     7021 tctccatcat aaactattgt atgtttgtac atctgtttaa ctccctcatt atttatattt
     7081 ttatttaata caccttctac aattaactta gcaatacctt catgacctag tttcttagct
     7141 ttctcataat cttctttatt atcacagaag aaactttcaa ttaatactgc tgtaggattt
     7201 gaactattta agatatataa tcctttatct aatttagctc ttctatttct aaatactgta
     7261 cctagcttat tacatattct agttgcatat tctaaacctt tattactata atatagaact
     7321 tctgaacctt ttccttgacc atcacttgca tttagatgta gttctataag taaatcatat
     7381 cctccactat taactctagg tattttataa gtcttttctt ctgctttagt tttaaactgc
     7441 ttttcagggc atattattac atctgcctta tgaccttctt ttctaaatgt atctgctaat
     7501 actggtgcaa gagatttatt gtattggtat tcgttaacta ctccaccagc agaagtgcat
     7561 gcaccacttt ttaaaatact gtgtcctact gttatacata ttttcattat ttattttcct
     7621 ccttcaactg tttgtaagtt tggtttatac ctattgatat accccaacaa atcacgcctt
     7681 gtaagactgc aacaggactt agtcctaaca tccaaataga aaatcctata ccaagtatta
     7741 ataacactac tggaatatat ttgttatcta gttgcttata cttcttacag cccttaccta
     7801 taatagagag agcagctact aaaattagca actgctctgg tataaaactt attaaattat
     7861 ccatctctta tcctcctaat taaaatattc ctctttgaac tgcaaatata aagaatccta
     7921 caagtgttgt aatcattgta ccaattagcc atttgagcat acttgtaagt gagtttagat
     7981 tctcacacaa tgcttttaac tctgctttag attctatatt tgctattttt aattcgtcta
     8041 tttcttctcc atgcttattt attcttgttt catgtctttt taaatctgct tcgaaaagtt
     8101 cttcattcat gtaagcctcc taatttttga attaaaaaag actatgctat atagtcctct
     8161 cctgttattt ctttatattc acttgctgtt atcttattct tttctactgc cgttttaact
     8221 tgctctttag tccaattacc attattatag aaatctgtta ttatcttgta ccaattcatt
     8281 tatatcaccc catttgacat taattgaaat gttaaatctg ctattgtttg ttctgtagaa
     8341 tttactttat cttctatgct acttttaata tctgtatatc tataaaacac ttctttagta
     8401 tctatattta taaataactt tgcttcttta ttttctacat atttttgagt tggtaatcta
     8461 tcaattaaag tacctttttt caaatcttct tctgacaata aacttgaatc ataatgtatc
     8521 atacaaacat attttatatt ttgttcttct gtgtccatta aatttcctaa aaaaatcata
     8581 attcctctcc tttttcatct gaataaacct tttttgataa tatatttctg taaatgccac
     8641 caccaataaa aaaaattata ttgtttatta taattatatt ttttgttggg gctacattat
     8701 aaaatgtgtt acaatagttg attgttttaa aagtatttaa atcctttttt tgaaaagagc
     8761 tactagagtt tggatatata acatatatat atatccatta tatatatcaa aatttgtaca
     8821 acggcttgaa tcagcataaa taatagggtt taaatttaca tcatacttga ctaatccgct
     8881 tattttacca ttagatattt caatttttcc actcatatca gatacaaaaa caaaatcatt
     8941 taaaaactta atattttttt catataaata tcctccaatt ctaaaatcct tagcaatagt
     9001 aaaatcaaaa tttattttag ttaggtaaca atctgtataa ccacttgaat tagcgtgttc
     9061 tgttgtagca taaatagcat tcttattgca aacaaactta cctctttcaa aatcgtaaat
     9121 actattgatt gacatatctt tagttaacat tttatacata tccgatattc ttattttgtg
     9181 aagtattgaa gaagtctcat ctccatatat tccataaata aattccccat aagtacatag
     9241 cttataataa gaaccttcta ttgattgcac ttcaattcct gttgtcttat ttattttata
     9301 taatttagta ttatcagata taaacaaata atcttgagta atgcaaatac atgagaaatt
     9361 atcattagac aaagtaatat caaaagttac agattcatca attgcattaa ttttaattaa
     9421 atgtgtacct ttaattacat aaaagaaagg ttcttcatat tctaatgctt ttaaatctcc
     9481 actatatttt tcaatatatt ttatagcacc attcataact aaatatgatg aactatttga
     9541 aagtattgtt tctcttaaat ctaatcttcc ttctttgttg tttactttat tctttatttc
     9601 ctcccaagtg ttatttgtag taatctctgc acccttggag ttcaatgctg ttactacatt
     9661 atttttagca ttaactccat tttgaaaaac ctcttttaat gctccttcta cattatcact
     9721 tgtaaagtta ttttctgtat cttctatagt tacattcttt gcttctaata caagatttct
     9781 aactttatta actaactctt taaaagtcat ttagtcacct tctttcaata aaaaaagaac
     9841 ctattttatt ggttctgctg gtgtttcttc tttatttaat aaacttgtaa gttctaaata
     9901 ttgttcttct gtaattctat ttactgcata gaatacatca attttgtgtt gcaaatcctc
     9961 tttagtgcta tagttctttt gttctatcat aagttttaat aagttataca tgttaattcc
    10021 tcctataaat tgttatttaa ttttatattt tctacctcaa aggctgtgtt tactatctca
    10081 ctatctctat ttttattttc ttcttttaac atgcttaatt ctttttctaa tgcttgtaat
    10141 ctcttttgtt catctgttaa aatgacttct atgtctttga tgattggttc ttttgtaact
    10201 ggatttatag actctatata ttgtttacta tagtctatac taccaaattc aacatccaaa
    10261 aaatttaatt cagttatttt tgaccactct tgtatatctc ctgttgcttc accagtttga
    10321 agccatatat tgcctgtttg gtcgtaaatt attctattat ttctgttcat attatcacct
    10381 cattttttta ttaaatatat attttgtaag ttaatgtagc tccctctttt gcccatattc
    10441 caactgcttc tgacattgat aatttatata atgtaagcac taatgaagaa tacccaacat
    10501 tagatacatt aagaatacca acagctttcg ggttactatg agtagaatca gaattatagc
    10561 caaaattata aaaatttgaa ttacaaacat tatcaaaata aacataagat ttacttgttg
    10621 tactaaaagt tactccacta agtacaatta aactaggaga gaagcccatg ttaactggta
    10681 tagtaagagt ttttgaagtt ccttctttgt catatatact taagttaaaa ttctctgcat
    10741 ccgtttttgt gaatgtataa gttcctgtta taaatctttt tcttttactt aactctgttt
    10801 ctaattgagt tatagtattg tttttttgtg ttacttgatt ttgtaaatct tgcacactag
    10861 tgtctgaact atcaaaactt gtttttattt tctctgataa ctccacaagt gtattattta
    10921 aacttgcttc tatattttta agtgctaaag tgtttataat acttgttttc ccaactttaa
    10981 atcctgcatt aacctcaact aattttgttg atatatcatt taaatttaca ttttcgggca
    11041 gtggcattat attcttactt atacttaaca ctttttctgc tgtagcatta ttactgtctg
    11101 taacaactat tttaagtgtg tgtaatgcat tatcttctaa tgtataatta attgttttct
    11161 cttctgttaa atttgttgtt atagtttctt ttagtacatc atctataaac cattctatct
    11221 ttgtaagtaa tgcagggtct gtgtggtcgg ctttaaatgt tgcctgtgta gaattataag
    11281 aagatactgt taaaaatggc aatgcttgta gtaatgttat tttagcataa ccatctgctt
    11341 tagtagtatt acctccagta gtcatgacta tattttcaag ataatattca ggtgttggta
    11401 tatatccggg tgccttataa ctatctttat ttagtgcgta accacttcca ccacctccac
    11461 attcattaga ataagaacca gcaccaccgt accaaccccc tccaccacct attcctatgt
    11521 taccataacc tccttttcct aatgaaccat ggtattcttc tgtgtcgtaa cttgttccac
    11581 cttggtattg agaaccaccg ccacaaaaat ctctgtcacg accaactcca ttaacaccta
    11641 cataaccacc accatgacca atagaacgag cagaagcaaa attatttttc atacctcctc
    11701 caccgcctgc aacaagtatg cgtgaaagca aactttcagt gttaccccaa gttgcactag
    11761 gatgataaag tcttatatca gttgctccac caccgtattt agaataagca aagctaccag
    11821 tagtaacttt gccagcagcg cctgcaccgt taaaaccact tctagtaagg cttgaacctt
    11881 cagaaacttt ctcataaccg gattgaccga cacaaatttg tagattagtt ctttttttaa
    11941 atacaatctc accttttgaa taaccacctt ttgcacaatc agtccaatcg cttgtatcga
    12001 cagcaccacc acaagcaccc caacattcta atttatatcg cccaggtggc aatgaaacat
    12061 tttgtacata attagcataa ttaaaattcc attcagtctg cattttctca ctctcctctc
    12121 taacaataag ttatcaactc atttacactt gttgcaatat tagataaacc accatttacc
    12181 ttttcttcta tattaaccaa tctgtcctct attttcttag atgaataagt agtcatttca
    12241 gatactctgt tatcatctac agttgcattt ataaaatgag tttctgcatt tccatttatc
    12301 acatagacat ttaactcaac ttttacttca cttctaatct caattgaatt atcatcaact
    12361 attttaaaat ttggaactat attttctttt gtagtagcat ctataatatt tacaactatt
    12421 ctatgtgtta ataaactatg tgttacagtt gctttgaatc cattttctgc atcctcaacc
    12481 caatcgtcaa ttgttattgt ttgagtagat gccacatttg aaccacctgc gattaattgg
    12541 tcaattttaa tattttgttt ttcattttct gtgtcaattc tagtgtttaa ctctgtttta
    12601 gtagtttcta tgttgcttgt taattctgtt ttagttgtat caattttaga gtctaaatct
    12661 tgtatatctt taagtgttgc taaaataaca gttgggtcta ctttcaaatt tacacttgaa
    12721 gtattagaga ctactaaaat aatttttata actaagtctt ttacactccc tgagtcagca
    12781 gttggtttgt atgtttctgg atatttagac actgcaatta gttgttcttt tgaatcaaat
    12841 atccctactt ctcttatcat aaaaccaccc acactacctg ggattacagt ttctattact
    12901 atccagtttg gattttcttc gtctatcttt acattattta cagaaccttc ccaaactaca
    12961 tttttcagag acgtttgttc ttctgttgga ttatattctg tccctgctcc atcaccaagc
    13021 tgcaatttaa ctaaatctac tttattgcct aaagctgttg catttgcaat agatgctttt
    13081 cctatcgttg tcaaaagtgt ataatatttt tgttctgcca acctatacca cctcctgtct
    13141 aggatataat gttacttttt ccatacttct atcattgcct gtagttatag tcaattctcc
    13201 aaaagaagtt atatcagttg gactgtaagg atatatagta actgtttcac ctgtattcat
    13261 tgtagaacct gcaaacatat cattttgatt aaataaaatc atttcaaatt tatgttcaag
    13321 atgtgcaggt tttatttctt ctattttctt atctaattct aaaatagtgt tataactaca
    13381 gtcatttgtt ataaaactaa gtgtaaaact aaatagatta ctaaatactt ttacatctac
    13441 gtctgttttt gtataagctt ctgatatagc ttttacaacc tctgctgtag ttgtaccttt
    13501 accacgcatc tttgctttta tattgcttct tctatcttct atatttaaat caaatctatt
    13561 tttaatagat aaaatatttt cccaataatc caatccccaa gtggctgtat ctacaaaaaa
    13621 ctggtcgaat gtatcatcat atgtttctct aagtgtgctc agttctatgt cataagcttc
    13681 ttgtatttta ctagtaatat cattgttata aaaagaaggt agcttatcaa ttaatttcaa
    13741 ttaaaccacc tcactaaact ctaaagtcgt aacacttggg actttatcct cttcaaatac
    13801 tatattttca gttttattat ttaataataa attactaaag tcatgtaagc cttctgtacc
    13861 tgcaagtatt gcacttactt tagtgtaaat tatttcttta ttaacattta ttaaatagct
    13921 attaatactc tctaagaagc tttcttttac aaaatctaat gtatatcccg cttctaattt
    13981 tatagatgca cttatactta tatctaaaac gcttggagtt aaaacggtta acgcaggacc
    14041 tattggcatt tcttcctcaa tatgttcttt gcatctttca attacttctg tgtcaacagc
    14101 ttgattattt tctccaaaga ttaaaacttt aattgttcct ggaccatccc atcttggata
    14161 aattttagcg ttatatactc cttcaacttc taaagcccat gcttcatagt gtgccttatt
    14221 tccacttgtt gcttgatttt tttgtatttt atagaatctt tcttttaatt cttcgtctgt
    14281 ttcgatttct gtaccacctt taaagtctaa attattataa attcttgtta taccgtttat
    14341 ttcgtctagc ttaaattctg tgtttgcagg tatattatat ctaattccaa tttctaaagc
    14401 ctgtacgggg cttatatttt gttcaatttc tgaactaatt actatatctt taattactac
    14461 gaataatagc tcattgtaag atataatagt tccgtttggt atgactgttc cgaccttccc
    14521 ctcgaatgtc acctctccag ttgcttctgt tcctaacttt ctatatactc caaactcatt
    14581 tacccgttta tcaagaaaat cgtcgaaatt atcctcaata aatgcttttt tatgaagata
    14641 tgaaagttct atatagaatt ttgctaactc actattaaca ggagatacca tatcacttaa
    14701 aaaagaaccc tcacccttat agatatctaa atttatattt gacaaggtcc tatttttcac
    14761 aacatcatat gtttgactac tatacattaa cttccacctc cccataaatc gtgtttatga
    14821 ttatatctac acttagtaaa tcatctgtaa attttgtatt cttgacattg acatctaata
    14881 tatatggatt aactaataaa gcctctttta tatatctact agcttcactt tctgtaagtc
    14941 ctttactata tttctgccct attagttcag ctaattctgt cccataaccc cagctataaa
    15001 t
//
LOCUS       DAYAPA010000107        14790 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.108, whole genome shotgun sequence.
ACCESSION   DAYAPA010000107 DAYAPA010000000
VERSION     DAYAPA010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14790
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.108"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            112..1902
                     /gene="dnaG"
                     /locus_tag="ACGI59_002927"
     CDS             112..1902
                     /gene="dnaG"
                     /locus_tag="ACGI59_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFG9740706.1"
                     /translation="MNDIKDIIEEIKARCDIASIISEYMNIKQSGANYKGLCPFHGEK
                     TPSFYINTSKQIYKCFGCGEGGDVINFVMKMENLDFMDAVKILANKCGIEINTNMNEE
                     TRIKIEKSKKFQDIHTEAARFYLSNLLGSKNLGYEYLRIRGLDDKIIKKFGLGFSLDS
                     WNSLMNTLISKGYKKQDLLECGLIAKNRDGTNCYDKFRNRVMFPIFDYRGNIIGFGGR
                     VLDDSLPKYLNSPDTLIFNKKQNLYGLNFARKNLESKTIVLVEGYMDLISLYQYGIKN
                     VVATLGTALTEQQGILIKRYADTAIISYDSDEAGIKATLRAIDILTKLGINVKVLDLK
                     DAKDPDEFVRKYGLSDYKKAMDVSTHYIKYKIDHLKKEFNIQKDEERVKFAKEASKII
                     KQLTSPVEIDFYTKYLSNQIDINVESIKREVYGKNYNKPYNNKNQKKIEEKVIEKVEV
                     RQDGKQLVEETLIKIMLEDKKIREIALLKVEESDFLLKESKEILNYMIKNQELDKITI
                     DKLKSLNISEEYLKELNSISLNSINLENTKEIEGIITNIRKNSLEEQINSLLREQQEL
                     ENNNDMKEVDGRVMEIALKIVEINKILKSL"
     gene            1920..3086
                     /gene="rpoD"
                     /locus_tag="ACGI59_002928"
     CDS             1920..3086
                     /gene="rpoD"
                     /locus_tag="ACGI59_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFG9740707.1"
                     /translation="MENKSNKKELKKVTAKTLIEKGKKQGSLTLAEIMEAFSETELDK
                     DQVENLYETLGNLGIEITETKNYKADIDFSVADDDLSIGHLDEDAEAISHDDSSAIEI
                     ETVDLSLPKGISIDDPVRMYLKEIGKIPLLKPHEEVEFARRMHEGDEIAKQRLVEANL
                     RLVVSIAKRYVGRGMLFLDLIQEGNLGLIKAVEKFDYTKGYKFSTYATWWIRQAITRA
                     IADQARTIRIPVHMVETINKLIRVSRQLLQELGRDPKPEEIAKEMEMTEDKVREIMKI
                     AQDPVSLETPIGEEEDSHLGDFIPDDDAPAPAEAAAYSLLKEQIEDVLGSLNDREQKV
                     LKLRFGLEDGRARTLEEVGKEFDVTRERIRQIEAKALRKLRHPSRSKKLRDYLD"
     gene            3251..3940
                     /locus_tag="ACGI59_002929"
     CDS             3251..3940
                     /locus_tag="ACGI59_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine(22)-N(1))-methyltransferase TrmK"
                     /protein_id="HFG9740708.1"
                     /translation="MKLTDRLLKIASLVSDGKKIADIGTDHGYIPVYLLKEGKVPFAV
                     LADVNKGPLDNAHKEVIQNNLLDKVDLRLGSGIEILEIGEVEEVIIAGMGGILISELL
                     EAKKEVAHNVEKLILQPMQAQEELRYYLLNNGYEILEEVLVREDFRIYEIIVAKYTGK
                     NTIIEDEIYYEVGIKLLENKDSLFNDFIEKKIKTYSSIVNKLEGKNGEAIDKKRKESE
                     VAIKKLENLIK"
     gene            3954..5051
                     /locus_tag="ACGI59_002930"
     CDS             3954..5051
                     /locus_tag="ACGI59_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFG9740709.1"
                     /translation="MLLKSLTRKIEKKYPLNLAEDWDNVGLIVGDFDMDVKKVLVSLE
                     ANEDVIDEAISKNIDLIVTHHPFIFGKINKINSGDLKGRLIQKLIKNDISLYSMHTNF
                     DIAFDGLNDYFMEIMEFGNSKVLDITKSENLYKLAVYVPHNYSDELRKVLSNSGAGHI
                     GNYSDCTFSTEGEGQFKPLEGSNPFLGSVNDIETVNEVKIETVVPQKLLGGVISSMLD
                     AHPYEEVAYDLYKLENKGEIFGLGRISKLDKSMTLESLSRKIKEKLNMKHIRVVGNLS
                     TDITKVAVVTGAGSEFVKKAKRQGAEVLITGDVKYHEAQDALDIGMCIVDCGHFDTED
                     IFKNVMKRFLDEFSEIEVIKSNVYLNPFSTI"
     gene            5114..5686
                     /locus_tag="ACGI59_002931"
     CDS             5114..5686
                     /locus_tag="ACGI59_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HFG9740710.1"
                     /translation="MKVAVIFHSVCGSTYLLAREYKEALEEMNIEVDIFRVSDEVAKT
                     LPQYYLINSKEYKDEFESINVIKSGKEILDYDAIFMGSPTYYGNVSGPMKMFMDSFSD
                     IWVGAPLSGKIFGCFATAGSQHGGGELALQAMNIFAQHMGMTLLSVPCSVRGGYPAYG
                     ILHIAGDNSDIRPNDDAKIGIRDYLKRLNI"
     gene            complement(5746..6213)
                     /locus_tag="ACGI59_002932"
     CDS             complement(5746..6213)
                     /locus_tag="ACGI59_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HFG9740711.1"
                     /translation="MFKEMRLKKREMTKEDTVEVLKNGEFGTFSTISENGYPYGVAVN
                     YVYFNDSIYFHCARNGHKLDNISKNNKVSFLVVANESVIPDKFSTTYSSAIVFGKACT
                     VENEEKKNALVEIIKKYSKGFFEEGMKYIEKDMNLTTVVKIEIDHISGKASRL"
     gene            complement(6599..7075)
                     /locus_tag="ACGI59_002933"
     CDS             complement(6599..7075)
                     /locus_tag="ACGI59_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFG9740712.1"
                     /translation="MLSKAEIKYFNECASEILSSEKVQLMRTFPHHGNVSCLEHSLSV
                     AYYSYLLCKKLHLSVDIQSVIRGALLHDFFLYDWHYKGDRKGLHGFTHPREALKNATL
                     FFQINEKETDIILKHMWPLTVKPPRYKEAFIVCLLDKFCCLVETLKIHSLLSPYHV"
     gene            complement(7195..8286)
                     /locus_tag="ACGI59_002934"
     CDS             complement(7195..8286)
                     /locus_tag="ACGI59_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5808 domain-containing protein"
                     /protein_id="HFG9740713.1"
                     /translation="MSKIECLFTIFPVLILMYATFYFIPYFVGKKHIYGVSIDQEHKN
                     YNYFIKLDKEFKKLLSLGFIIDFILVFILVFTFNKLELSYFIGIIGFLLYESILYIHT
                     HKKAKKLKSEIYAKLGHIDIDSKLIVDMDFINKKNKIIKKFKIIYLIPILFTLGMSIF
                     IAFNYNQLPDSIATHWNINGSPDVFIDKSFLNVFKLIGTDFCLMVLLYITSIGSIKSR
                     IKIDTNRIEESRVENIKYLNKIGYLFLILMIIMTTQFFTTLLSIKTKLLTAMNITTLL
                     VIIYLIATYINSPNLKFNSSYSPEEDEKYWIAGIMYNNPNDPSLMVNKRFGIGWTINF
                     GNPLGKILYIAIALLLIFSLFSLIKSLLL"
     gene            complement(8279..8668)
                     /locus_tag="ACGI59_002935"
     CDS             complement(8279..8668)
                     /locus_tag="ACGI59_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFG9740714.1"
                     /translation="MVLNLDFNSDKSIYVQIKEEITKAIASGELKINESLPSVRSMAE
                     NIGVNLHTVNKSYNELKEEGFLNIDRRKGAIVAQLPMKIDNTTRQKNKLDLELLVAKS
                     YLSGISKEEFLSLSSSLFDKYEVKYYE"
     gene            8860..9306
                     /locus_tag="ACGI59_002936"
     CDS             8860..9306
                     /locus_tag="ACGI59_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9740715.1"
                     /translation="MLTYLMKRQMHKKDDGMSKPMMITGAVITGVGAYAVYKMVKSHK
                     SNSQMVNTNYYGNSDYDDYKYDEFDYDCDCRDTKQDYEYEELKKKVKEFNSRRINCEN
                     CPHVSQEEMMQYVNSVKDVKEELDLHEDAKKDNIYKEDEYKKYEEE"
     gene            9591..10295
                     /locus_tag="ACGI59_002937"
     CDS             9591..10295
                     /locus_tag="ACGI59_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFG9740716.1"
                     /translation="MLKLLIVEDDSTISFGIKYALEQEGFSIDISKDLSSGKEMISSN
                     EYSMILLDVTLPDGTGYELCQYIRGFSQVPIIFLTACDEEVNIVMGLDIGGDDYITKP
                     FRIRELISRIKAVLRRKGNTSEENKKILKFGDLSIYTLEARVYKNDKEIFLTSVEYKL
                     LLILIQNKNTVLSRTQILEKLWDVTYDFVNDNTLTVYIKRLREKIGDDLCEPIYIETV
                     RGIGYKWIGSENSVSI"
     gene            10273..11526
                     /locus_tag="ACGI59_002938"
     CDS             10273..11526
                     /locus_tag="ACGI59_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFG9740717.1"
                     /translation="MKIVFLYNPEVKKFLSKYVTLMFIVIIISIGFSIINVSLTKEMV
                     VRNNQAIIGTLSSKYPNLESQIVDIITQGKSMENIDYGQKILIKYNYDKSIRINSEPI
                     ISKLVLETIKINIILVCIIFILIFVLIVRYFTSIYNDLSDMTKYVYYSSEGKPFDMKN
                     KNQEGQIGLLKTELLKMTTILNEKVELLKREKIFLNNTISDISHQLKTPMTSLIMLND
                     LLYNDVPYEVKIDFLNKIKNQLNRMDWLIKSMLKLSKVEAKVINFKKDKVKFSELIHR
                     AMLSMKIPMEIKNQKLTIEGNDNVSYIGDIDWSVEALVNIIKNCVEHTPEFGNIIITY
                     KENPLFSELIIKDEGEGIDKKDIPHVFKRFYRGRSSSKEDSVGIGLAMSKSIIESQNG
                     DIYVNSEKGKGTEFHIIFHKMYDSD"
     gene            11606..12283
                     /locus_tag="ACGI59_002939"
     CDS             11606..12283
                     /locus_tag="ACGI59_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9740718.1"
                     /translation="MEILRVENLTKSYGKNETKVDAIKNVSLSVEKGTFIAITGPSGS
                     GKSTLLHLLGGVDRPTSGKVYINDVDIYNLKTKDLAIFRRRNIGLIYQFYNLIPVLNV
                     KENILLPAELDNRDIDREYFDDLMKTLGLIDREKHLPNQVSGGQQQRTSIGRALINRP
                     SIILADEPTGNLDSKNSKEILELLKLSVKKYNQTLIMITHDKNMALQADRIISIEDGI
                     IKSDEVM"
     gene            12284..>14790
                     /locus_tag="ACGI59_002940"
     CDS             12284..>14790
                     /locus_tag="ACGI59_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9740719.1"
                     /translation="MNLYTSLTIRYLKQNKRRTIVTIIGIILSTALICGIGNIFESFM
                     DYQMRETIKNDGSFHVTFYDVNRKNVEYVTKSAEIEKHAFTKQLGYSKLENSENAILS
                     IKQYDKNAINGYKVSIKEGRFPAKEGEIVLSESIINLIDDKLKIGDKITLKVGDMFDS
                     SKKKIDSMTFHEGDYIANEKDRTFKVVGIIEKPGFERYNGIATAIAYFNPMNNYNDTI
                     NVSVAVKNPKDVYKISNKISKNIYSNKDKEAVSDMIKYNEHLLRLQGASKYSNINSSI
                     KSIIIAVTILVIVCTIATVYNSFSISINERKKQFGILNSIGATSSQIKRLVFIEGIII
                     SLIGIPIGLISGTVAIDLLFKIINKYFTESVVTKMSLQIVYNPIIIIVSIIIVLLTIF
                     ISILLPAISASNISPLNVIKNTGEYKVGKIKSSRLIKMIFKTEGVLAYKNIRRNRKKF
                     IITLFSLMVSIIIFISFSGFTLLLLRGEEIRNSQRKYDLYLTAKNTARSVEDTISELE
                     DIDGIKNFELATGQYVSIRVSENKINKSKEDLIRKYYQKYKIGDSYEYDFSNNELIFP
                     GDFAVKNKINNLVQGSFNKERAIEENGVILVRKSAIEENGKKGVVELTNYKVGDTVNC
                     EYLDENALSKKVKVKILAITDEERLGLGYQNMGLQFITYDEVAKNLNLKLSKSLIFID
                     SGGSIKTKKEIEALANKNNFNFYDESASNESEKQDLKVIKIFVYGFIVVISLVSVTNI
                     LNTVSTSINLRKRELAVIQSIGVTPKGFRKMIYLESFIYGILSLLFGIPISIGIILIM
                     NKLISGVIEFSPIIPWTAIVICIVSVFIITFIAGYIP"
BASE COUNT         6049 a         1534 c         2474 g         4733 t
ORIGIN      
        1 gaaggatatc aataaaaaaa gtcgaattgt gtatattgag tgattgacaa atttagaata
       61 tagtgtagaa tagaaatgtt aacacaaaaa attacaccag aaggtgataa tatgaatgat
      121 ataaaagaca ttattgaaga aataaaagct agatgcgata tagcaagtat aatatctgaa
      181 tatatgaata taaaacaatc tggagctaat tacaaaggat tatgtccatt tcatggcgaa
      241 aagacacctt ctttttatat aaatacatca aaacagattt ataagtgttt tggttgtggt
      301 gaaggtggag acgtaataaa ttttgtcatg aaaatggaaa atttggattt tatggatgct
      361 gtaaaaattt tagcaaataa atgcggtata gaaattaaca ctaacatgaa tgaagaaact
      421 aggattaaaa tagaaaaatc taagaaattt caagatattc atacggaagc agctagattt
      481 tatctttcaa acttattggg aagcaaaaat cttgggtatg aatatttaag aataagagga
      541 ctagatgata aaattattaa gaaatttggt ttaggatttt ctttggattc atggaattca
      601 cttatgaata ctctaatcag taaaggatat aaaaaacaag acttattaga gtgcggtcta
      661 attgcaaaaa atagagatgg aactaattgc tatgataaat ttagaaatag agttatgttt
      721 cctatattcg attatagagg aaatataata ggatttgggg gaagagtatt agacgattct
      781 ttgccaaaat acttaaattc tccagacaca ttaattttta ataaaaaaca gaacttatac
      841 ggattaaatt ttgctagaaa aaatttagaa agtaaaacta tagtcttagt tgaaggatat
      901 atggatttaa tttcacttta tcagtatggt ataaaaaatg tagtagcaac ccttggaaca
      961 gccttgactg aacaacaagg cattttaata aagagatatg cggacactgc tattatatct
     1021 tatgattctg atgaagcagg aataaaagcg actttaagag ctatagatat acttactaaa
     1081 ttaggtatca atgtaaaagt gttagactta aaagatgcta aagacccaga tgaatttgta
     1141 agaaaatacg gacttagtga ttataaaaaa gctatggatg tttctactca ctacataaaa
     1201 tataaaatag atcatcttaa aaaagaattt aacattcaaa aagatgaaga acgagtgaag
     1261 ttcgcaaaag aagcttctaa aattataaaa caattgacaa gtcctgttga aattgatttt
     1321 tatacaaagt atttaagcaa tcaaatagat attaatgtcg agtctataaa gagagaagtt
     1381 tatggtaaaa attacaataa gccatataat aacaaaaatc aaaagaaaat agaagaaaaa
     1441 gtaattgaaa aagtcgaagt cagacaagac ggaaaacaat tagttgaaga aactctaata
     1501 aaaataatgt tagaagacaa aaaaattaga gaaatagcgt tacttaaagt agaagaaagt
     1561 gattttttat tgaaagaaag taaagaaatt ttaaattaca tgattaaaaa tcaagaattg
     1621 gacaaaataa ctattgacaa attgaaaagt ttaaacatat ccgaagaata cttaaaagaa
     1681 ttaaattcaa tttctttgaa tagtataaac ttagagaaca caaaagaaat agaaggaata
     1741 ataacaaata tcagaaaaaa ctcattagaa gagcaaatta atagcttgtt gagagaacaa
     1801 caagaattag aaaataataa tgatatgaaa gaggtagatg gtagagttat ggaaattgct
     1861 ttaaaaatag ttgagataaa taaaatttta aagagcttgt agagaaagga ggtttgagtg
     1921 tggaaaataa atcgaataaa aaagagttaa aaaaggttac tgctaagaca ttaatagaaa
     1981 aaggaaaaaa gcaaggttct ctgacacttg cagagataat ggaagctttt tcggagactg
     2041 aacttgataa ggatcaagta gaaaatcttt atgagacttt aggaaatttg ggaatagaaa
     2101 taacagaaac aaaaaactat aaagctgata tagatttttc ggttgctgat gacgatttaa
     2161 gtataggcca cttagatgaa gatgcagagg caatttcaca tgatgactct tctgcaatag
     2221 aaatagaaac tgtggattta tctttaccaa aagggataag tatagatgac cctgtaagaa
     2281 tgtacttaaa agaaatagga aaaattcctc tacttaaacc acatgaagaa gtagaatttg
     2341 ctagaaggat gcacgaaggt gatgagatag caaaacaaag gttagttgaa gcaaacttaa
     2401 gactagttgt aagtatagca aaaagatatg taggaagagg tatgcttttc ttggatttaa
     2461 tacaagaggg aaatttaggt cttataaaag cagtcgaaaa atttgactat acaaaaggat
     2521 ataagtttag tacttatgca acatggtgga taagacaagc tataacacgt gctattgcag
     2581 accaagctag aactataaga ataccagttc atatggtaga gactataaat aagctaataa
     2641 gagtatcaag acaactactt caagaacttg gaagagatcc aaaaccagaa gaaattgcaa
     2701 aagaaatgga aatgacagaa gataaagtaa gagaaataat gaaaatagct caagaccctg
     2761 tgtctttaga aacaccaata ggagaagaag aagatagcca tttaggtgac tttattccag
     2821 atgatgatgc tccagcccca gcagaggcag cagcatattc tttattaaaa gaacaaatag
     2881 aagatgtact tggttcatta aatgatagag aacaaaaagt attaaagctt agatttggtc
     2941 ttgaggatgg tagagccaga actcttgagg aagttggaaa agagtttgat gtaactagag
     3001 agagaataag acagatagaa gcaaaagcac taagaaaact aagacatcca agtagatcaa
     3061 aaaaacttag agattattta gactaaaaag atacatcatt gatgtttttg tgataaatgc
     3121 catgagataa gtgatatctc atggctattt tgtagtaaag atttaatttt atttttgtag
     3181 ttatttcagt ctaatatcta taacttggat taaatcctat aaataaaggg aattgaaagg
     3241 agaattatat ttgaaattaa cagatagatt attgaaaata gcatcattag tgtcagatgg
     3301 taaaaaaata gctgatatag gaacggacca cggatacata ccagtatatt tgttaaaaga
     3361 gggaaaagta ccatttgcag tgctagcgga tgttaataaa ggacctcttg acaatgcaca
     3421 taaagaagtc atacagaaca atcttttaga taaagttgat ttacgattgg gttctggtat
     3481 agagatactt gaaatagggg aagtagaaga agtgataatt gctggtatgg gtggaatctt
     3541 gataagtgag cttctcgaag ctaaaaaaga agtagcacat aatgtagaaa aattaatatt
     3601 acagccaatg caagcacaag aagaacttag atattacctt ttaaacaacg gatatgaaat
     3661 tttagaagaa gttttagtga gagaagattt tagaatatat gagataattg ttgccaaata
     3721 tacaggtaaa aatactataa tagaagatga gatatattat gaggttggaa taaagctttt
     3781 agaaaacaaa gattctcttt ttaatgattt tatagaaaaa aagataaaga catatagttc
     3841 tatagttaat aaattagaag ggaaaaatgg ggaagctata gataagaaga gaaaggaaag
     3901 cgaagttgca ataaaaaaac ttgaaaattt aataaaataa ataggaggta tatatgctat
     3961 taaagagtct aactagaaaa attgaaaaaa agtatcctct aaacttagca gaggattggg
     4021 ataatgtagg tcttatagtt ggagattttg atatggatgt aaaaaaggta ttggtatctt
     4081 tagaagctaa tgaagatgtt attgatgaag ctatatccaa aaatatagat ttaattgtga
     4141 cacatcatcc ttttatattt gggaaaatta ataaaattaa tagtggagat ttaaaaggca
     4201 gacttatcca aaaacttatt aaaaatgata tatcacttta ttctatgcat acgaattttg
     4261 atatagcatt tgatggtcta aatgactatt ttatggaaat tatggaattt ggtaattcaa
     4321 aggttttaga tataactaaa agcgaaaatt tgtataaatt agcagtatac gttccacata
     4381 actattcaga tgaacttaga aaggttttaa gtaattcagg agcaggtcat atagggaatt
     4441 atagtgattg tacgttttca acagaaggtg aaggacaatt taagccatta gaaggctcta
     4501 atccgtttct tggaagtgta aatgatatag aaactgtaaa tgaagttaaa attgaaactg
     4561 tagtaccaca gaagctttta ggtggggtaa taagttctat gttagatgca catccttatg
     4621 aagaagttgc atatgatttg tacaagttag aaaataaagg tgagatattt ggactaggta
     4681 gaataagtaa acttgataaa agtatgacat tagaaagtct ttcaaggaaa ataaaagaga
     4741 aattaaatat gaaacacata agagtagttg gaaaccttag tacagacata acgaaagtag
     4801 ccgttgtaac aggagctggt tcagaatttg taaaaaaagc taagagacaa ggtgcagaag
     4861 tactaattac gggtgatgta aagtatcatg aggcacaaga tgcacttgat attggaatgt
     4921 gtatagtgga ttgtggtcat tttgatacag aagatatatt caaaaatgtt atgaagagat
     4981 ttttagatga atttagtgaa attgaagtaa taaagagtaa tgtatatttg aatccattta
     5041 gtactattta gcagtgtttg ataataaaaa tactatataa aaaataaatt tgagtttaat
     5101 tagggggata atgatgaaag tagcagtaat ttttcatagt gtttgtggga gtacatattt
     5161 attagctaga gaatataaag aagcgcttga agaaatgaat atcgaggtag atatatttag
     5221 agtaagtgac gaagttgcaa aaacacttcc tcaatattat ttgataaatt ccaaagaata
     5281 caaagacgaa tttgaaagta taaatgtaat taaatctgga aaagaaatat tagattatga
     5341 tgctatattt atgggttcac caacatatta tggaaatgta tctggaccaa tgaagatgtt
     5401 tatggatagt ttttctgata tttgggtagg tgctcctttg agtggtaaaa tatttggttg
     5461 ttttgctaca gcaggttcac aacatggagg aggagaatta gctttgcaag ctatgaatat
     5521 atttgcccaa catatgggaa tgactctttt gtcagttcca tgttcagtaa gaggaggtta
     5581 tccagcgtat ggaatacttc atatagcagg agataattct gatataaggc caaatgatga
     5641 tgccaagata ggtataagag attacctaaa aagacttaac atataagaat atcatataaa
     5701 aatacctaaa agcgactgta tatactttta ggtattttta cttaattata aacgtgaagc
     5761 tttaccagaa atatggtcaa tttctatttt aacaacagtt gttaagttca tatccttctc
     5821 tatgtacttc atgccttctt caaagaatcc tttagaatat ttttttatta tttctacaag
     5881 agcatttttc ttttcttcat tttctacagt gcaagctttt ccaaaaacta tagcactaga
     5941 ataagtagta ctaaatttat ctggaataac actttcatta gcaactacta aaaaagaaac
     6001 tttgttgttt ttcgatatat tatctaactt atgcccattt cttgcacaat gaaagtaaat
     6061 agaatcatta aagtatacat aatttacagc aactccataa ggataaccat tttcagatat
     6121 agtggaaaaa gtaccaaatt caccattttt taatacttca acagtatctt cttttgtcat
     6181 ttctcttttt ttcaatctca tttctttaaa cataatatca tcctttctaa ttttgatgct
     6241 taaattaaat tttagaacat ttataaagat tatgctatgt tttttacaaa ttttaagaaa
     6301 aaaatatagt ttaagataaa aggtagtgaa agagaagcta tttggaggtt tatttatgat
     6361 attattgtta gagtagtaca aataatacta ataaatttaa tagctggttt cttgttattt
     6421 aaggtagtaa gcatgtctat aagaagggga agttatcgag ggtagaagtt gttgtttata
     6481 acgatattat aaagaaaata ttattatata aaataataaa tagtaaagag gtatcaagtt
     6541 gaatataata cttaatacct cttcaaaatt aagttataaa agtgttgtgg gaattgtcct
     6601 atacatggta aggagataat aaagaatgta tctttaaagt ctcaactaaa cagcaaaatt
     6661 tatccaacaa acatacaata aatgcttctt tgtatctagg tggcttaaca gtaagtggcc
     6721 acatgtgttt taaaattatg tccgtttctt tttcatttat ttgaaaaaat aaagttgcat
     6781 tttttaatgc ttctctaggg tgagtaaatc catgtaatcc ttttctatca cctttgtaat
     6841 gccaatcata aagaaaaaag tcgtgtagta aagctcctct aataacactt tgaatatcaa
     6901 cacttaagtg caatttttta cataatagat aactataata tgctactgac aaactatgtt
     6961 caagacatga aacattgcca tgatgaggaa atgttctcat aagttgaact ttttcagaag
     7021 aaagtatctc agaagcacat tcattaaaat atttgatttc agcttttgac aacattatga
     7081 aaatctccat tcttagtaat atataaatac tatacttcaa cgataatatg aaatatttct
     7141 tatcctacaa aatataatac ccaataatac aagtaagata ccttttatgg tattttaaag
     7201 taagagagat tttattaggc taaacaatga aaaaattaaa agtaaagcta tagcaatata
     7261 tagtattttt ccaagaggat tgccaaaatt tatagtccat ccaataccaa accttttatt
     7321 aaccattaat gatgggtcat ttggattatt atacatgatg cctgctatcc aatacttctc
     7381 atcttcttct ggagaataag atgagttgaa ttttaaattt ggggaattaa tatatgttgc
     7441 tattaaataa attataacta aaagcgttgt aatattcata gcagttaata gttttgtttt
     7501 tattgataaa agagtagtaa aaaactgtgt tgtcattata atcatcaata ttaaaaacaa
     7561 ataaccaatt ttatttaaat atttaatgtt ttcaactcta ctttcttcaa ttctatttgt
     7621 atctatctta atccttgact ttatggatcc aatggaagtt atatataata aaaccattaa
     7681 acaaaagtca gtaccgatta atttaaatac attaagaaat gatttatcta taaaaacatc
     7741 aggagaccca tttatgttcc aatgagtggc aattgagtct ggcaattgat tataattaaa
     7801 tgctataaaa atactcattc ccaaagtaaa tagtatcggt attaaataga ttattttaaa
     7861 tttttttatt attttatttt tcttatttat aaaatccata tctactatta attttgaatc
     7921 tatatctata tgacctaatt tagcataaat ttctgattta agcttcttag cttttttatg
     7981 tgtatgaata tatagtatac tttcatataa taaaaaacct ataataccaa taaaatagga
     8041 aagttccaat ttattaaatg taaagaccag aataaaaact aatataaagt ctataataaa
     8101 acctaaagat aatagttttt taaattcttt atctaacttt ataaagtaat tataattttt
     8161 atgctcttgg tctatagaaa ctccatagat gtgtttttta cctacaaaat agggtataaa
     8221 ataaaatgta gcatacatca aaattagaac tggaaatatt gtaaatagac attcaatctt
     8281 actcataata tttcacctca tacttgtcaa ataaacttga acttagtgac aaaaattctt
     8341 ctttactaat acctgataaa taagattttg ccaccaataa ttccaagtca agtttatttt
     8401 tttgacgagt tgtattatct attttcattg gtagttgagc tacaatagca ccttttcttc
     8461 tatcaatatt taagaatccc tcttctttta attcattgta ggatttgttc actgtatgta
     8521 aatttacacc aatattttca gccatacttc ttacagatgg aagtgattca tttattttca
     8581 attctcctga agctatagcc ttagttattt cttctttaat ttgtacataa atagatttat
     8641 cactattaaa atctaaattt aaaaccataa aaaacctcca ttatgcttta taaatgaaaa
     8701 cgattgttat atacatagta taacataaaa aataataaca aacacaaaat ataagtttat
     8761 attttgtaaa aattgtaatt taaaaaataa cataattttt attaaaataa tacagatact
     8821 atttatagat agaaataaaa attagaaagg agaaaaaata tgttaacata tttaatgaaa
     8881 agacaaatgc ataaaaaaga tgatggaatg agtaaaccaa tgatgattac aggagctgta
     8941 attacaggtg taggagcata tgctgtatat aaaatggtaa aaagccataa atctaattct
     9001 caaatggtca atacaaatta ttatggaaat agcgattatg atgattataa atatgacgaa
     9061 tttgattatg actgtgattg tagagacaca aaacaagatt atgaatatga agaattaaaa
     9121 aaaaaagtaa aagaatttaa ttcaagaaga ataaattgtg aaaattgtcc acatgtttct
     9181 caagaagaaa tgatgcaata tgtaaatagt gttaaagatg taaaggaaga gcttgattta
     9241 catgaagatg ctaaaaaaga taatatttac aaagaagatg aatacaaaaa atatgaagaa
     9301 gaataagtaa tattttgtat aactgaacaa atagtaatgt caaattattt aatatctagt
     9361 tttaatatgt aagttgtaac ttaacttgaa agaatcagta tcattcttta gatagttaag
     9421 taacaactta cattttattt ttataaattt taatagttct ttgtgactat tttgtaattt
     9481 tagaaatatt ttagtatcca gggtaattgt gtataataaa acttgtatag atagatagta
     9541 tgaaattaag cagatagttt aaaattaaaa tgaaaagaaa ggaaatagtt atgttaaagt
     9601 tgttgatagt tgaagatgat agtactatat cttttggaat aaagtatgct ttagaacaag
     9661 aaggatttag tatagatata tcaaaggatt tatctagtgg aaaagaaatg atttcttcta
     9721 atgaatatag catgatactg ttggatgtta cattgccaga tggaactggt tatgagcttt
     9781 gtcaatatat aagaggattc tctcaggttc ctataatatt cttgactgct tgtgatgaag
     9841 aagtaaatat tgtaatgggt ctcgatatag gtggagatga ttatataaca aaacctttta
     9901 gaattagaga attaatatcc agaataaaag ctgttttaag aagaaaaggt aatacttcag
     9961 aagaaaataa gaaaatactt aaatttggag atttaagtat atatacatta gaagctagag
    10021 tatataaaaa tgataaagaa atatttctga catcagttga atataaactt cttttaattc
    10081 ttatacagaa taaaaatact gttttaagta gaactcaaat attggaaaaa ctttgggatg
    10141 taacttatga ttttgtaaac gacaatactc taactgttta tataaaaagg cttagagaaa
    10201 aaataggtga tgatttatgt gaaccaatct acatagaaac tgtaaggggg attggttata
    10261 aatggatagg aagtgaaaat agtgtttcta tataatccag aagtgaagaa gtttttaagt
    10321 aagtatgtta ctttgatgtt tattgttatc ataatatcta ttggatttag cataatcaac
    10381 gtgtccttaa ctaaagagat ggttgtaaga aataatcagg ctataatagg aactttatca
    10441 agcaagtatc caaacttaga aagtcagata gtagacataa taactcaagg gaagtctatg
    10501 gagaacatag attatggtca gaaaatatta attaagtata attatgataa atctattagg
    10561 ataaactctg agcctattat ttctaaatta gttttagaga ctatcaaaat aaatataata
    10621 ttagtatgta taatatttat tttgatattt gtgttgattg ttagatattt tacgtcaatt
    10681 tacaatgatt tatcagatat gactaagtat gtttactata gttcagaggg taagccattt
    10741 gatatgaaaa acaagaatca agaagggcag ataggtcttc taaaaactga actcttaaaa
    10801 atgacgacaa ttcttaatga aaaggttgaa cttttaaaaa gagaaaagat atttttaaac
    10861 aatacaattt ccgatatatc acatcaatta aaaactccta tgacttcttt aataatgttg
    10921 aatgatttat tatataatga tgtaccatac gaagtaaaaa tcgatttttt aaataagata
    10981 aaaaatcaat taaatagaat ggattggttg attaaaagca tgcttaaatt atcaaaggta
    11041 gaagcaaaag tgataaattt taaaaaagat aaggtaaaat ttagtgaact catacataga
    11101 gcaatgctat ctatgaaaat acctatggag attaaaaatc aaaaactgac tatagaggga
    11161 aatgataatg tatcttatat tggtgatatt gattggtccg tggaagcatt agtgaatata
    11221 atcaaaaatt gtgtagaaca tactcctgaa tttggaaata taattataac ctataaagaa
    11281 aatccattat tttctgaact aataataaaa gatgagggtg aaggtataga taaaaaagat
    11341 ataccacatg tatttaaacg attctataga ggaagaagta gttcaaaaga agatagtgta
    11401 ggaataggtc ttgcaatgtc aaaatctata atagaaagcc aaaatggcga tatatatgta
    11461 aacagcgaaa aaggtaaggg tacagaattt catataatat ttcataaaat gtatgatagt
    11521 gactaatctg taatatttaa ttcactttat agtaatagcc aaaacctaat atatgaagta
    11581 tagaatataa atattggagg tagttatgga aattttaaga gtagaaaatc taactaaaag
    11641 ttatggtaaa aatgaaacaa aagttgatgc tataaaaaat gtaagtttat cagttgaaaa
    11701 aggaacgttt atagcaataa caggtccaag tggaagtggt aaaagtacct tattacattt
    11761 attaggagga gttgacagac caactagtgg taaggtttat ataaatgatg ttgatatata
    11821 taatttaaaa actaaggatt tggcgatatt tagaagaaga aatataggtc ttatatatca
    11881 attttacaac ttaattccag tcttaaatgt gaaggaaaac atattgcttc cagcagagct
    11941 tgacaataga gatattgata gagaatattt tgatgattta atgaaaacat taggtctaat
    12001 tgatagagaa aagcaccttc caaaccaagt atcaggaggt caacaacaga gaacctctat
    12061 aggacgtgca ttaattaata gaccttctat aatattagca gatgagccaa caggtaattt
    12121 agatagtaaa aattcaaagg aaatattaga attgttaaaa ttgtcagtaa aaaaatacaa
    12181 ccagacacta ataatgataa ctcatgacaa aaatatggca cttcaagctg atagaattat
    12241 aagcatagaa gatggaatca taaaaagtga tgaggtgatg tagatgaatt tatatacatc
    12301 actaactatt agatacttaa aacaaaataa aagaagaacc attgtaacca taatcggtat
    12361 aatactttca acagctttaa tttgtggtat aggaaatata tttgaaagtt ttatggatta
    12421 tcaaatgaga gaaactataa agaatgatgg aagtttccat gttacatttt atgatgtaaa
    12481 tagaaaaaat gtggaatatg taacgaaatc agctgaaata gaaaaacatg catttaccaa
    12541 acaacttgga tattctaaat tagaaaatag tgaaaatgct atattaagta taaagcaata
    12601 tgataaaaat gcaataaatg gatataaagt ttctataaaa gaaggtagat ttccagctaa
    12661 ggaaggagaa attgttttaa gtgaaagtat tattaatctt atagatgata agttaaaaat
    12721 aggagataag ataactttaa aagtagggga tatgtttgac tcaagcaaga aaaagattga
    12781 tagtatgaca tttcatgaag gtgattatat agcaaatgaa aaagatagaa cttttaaagt
    12841 tgttggtata attgaaaaac ctgggtttga acgttataat ggaattgcaa cagctatagc
    12901 ctactttaat cctatgaata attacaatga tacaattaat gtatcagtag cagtaaaaaa
    12961 tcctaaagat gtgtataaga taagtaataa aataagtaaa aatatatatt caaataaaga
    13021 taaagaagca gtttctgata tgataaaata caatgaacat ttattgaggt tacaaggtgc
    13081 tagtaagtat tcaaatataa atagctctat aaagtctata attatagcag taactatttt
    13141 agtaatcgta tgtacaattg ctacagtgta caattccttt agtatttcta taaatgaacg
    13201 aaaaaaacag tttgggatat taaattctat aggtgctaca tctagtcaaa ttaaaagatt
    13261 agtttttata gaaggtatta taattagttt aattggtata ccaataggac taatatctgg
    13321 aacagtagct atagacttat tgtttaaaat tataaataag tattttactg aatcggttgt
    13381 tactaagatg agtttgcaga tagtgtataa tccaataata attattgtta gtataataat
    13441 agttttgctt acaatcttta tatcaattct actacctgca atatcggctt ctaatatttc
    13501 tccccttaat gtgattaaaa atactggaga gtataaagtg ggcaaaatca aaagctcaag
    13561 acttattaaa atgattttta aaactgaagg agtacttgca tataaaaata ttaggagaaa
    13621 tagaaaaaaa tttataataa ctcttttttc tttaatggta agtataataa tatttatatc
    13681 ttttagtggc tttacattac ttttactaag aggtgaagaa attagaaatt ctcaaagaaa
    13741 atatgatttg tatttaactg caaagaacac agctaggtct gtagaggata ctataagtga
    13801 actagaagat attgatggga taaaaaactt tgaattggca acaggacaat atgtgtctat
    13861 aagagtaagt gaaaataaaa taaataaatc caaggaagat ttaatcagga aatattatca
    13921 aaaatataag attggagata gttatgaata tgatttcagt aataatgaat taatttttcc
    13981 aggagatttt gcagtaaaaa acaaaataaa taacttagta cagggctctt ttaataaaga
    14041 gagagcaatt gaagaaaatg gtgttatatt agttagaaaa agtgcaattg aagaaaatgg
    14101 gaagaaaggt gtagtagaac ttaccaacta taaagtagga gatacagtaa attgtgaata
    14161 tcttgatgaa aatgccttaa gtaaaaaagt aaaggtcaag attcttgcta taactgacga
    14221 agaacgtcta ggtcttggtt atcaaaatat ggggttacaa tttataactt atgatgaagt
    14281 tgctaaaaat ttaaatttaa aattaagtaa aagccttata tttattgatt caggaggaag
    14341 cattaagact aagaaggaaa tagaagcttt ggcaaataaa aataatttta acttttatga
    14401 tgaaagtgca agcaatgaaa gtgaaaaaca agatttaaag gttattaaaa tatttgtata
    14461 tggttttata gtagtgatat ctttagtgag tgttacaaat attctaaata cagtaagtac
    14521 aagtataaat ctaagaaaaa gagaacttgc agtaatacaa tctatagggg ttactcctaa
    14581 aggatttagg aaaatgatat atttagaaag ttttatatat ggtatactgt cattgttgtt
    14641 tggaatacca attagtattg gtataattct aattatgaat aaacttattt caggtgtaat
    14701 tgaattttct ccgattatac cgtggacagc catagttatc tgtatagtaa gtgtttttat
    14761 aataactttt atagcagggt atataccaat
//
LOCUS       DAYAPA010000108        14669 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.109, whole genome shotgun sequence.
ACCESSION   DAYAPA010000108 DAYAPA010000000
VERSION     DAYAPA010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14669
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.109"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            115..270
                     /locus_tag="ACGI59_002941"
     CDS             115..270
                     /locus_tag="ACGI59_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9740720.1"
                     /translation="MKGILKELTAKLYSFFYFMFWGFYFSAGYFFYKNICTSFLVRPY
                     FSDFEHK"
     gene            400..1413
                     /gene="aroF"
                     /locus_tag="ACGI59_002942"
     CDS             400..1413
                     /gene="aroF"
                     /locus_tag="ACGI59_002942"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HFG9740721.1"
                     /translation="MIVVLKMGADKNEVKKLIEAIGREGVEVNPIDGTELTVLGLVGD
                     TSKIDAKRIEANKVVEKVMHVVEPFKKANRKFHPEPSIINVNGMEIGSKKIAMIAGPC
                     SVETEDQIVSIAKDVKKSGAGFLRGGAFKPRTSPYAFQGLKYDGLDLLKKAKEKTGLP
                     IVTEIMSTQDIDIFEENVDVIQVGARNMQNFDLLKELGKTNKTILLKRGLSATIEEWL
                     MSAEYIMAGGNENVVLCERGIRTFETYTRNTLDLSAILAVKKLSHLPVIVDPSHAAGK
                     SWMVDSLSKAAIAVGADGLIIEVHNDPAHALCDGKQSIKPNEYDELISELKTIASAVG
                     REI"
     gene            1435..2499
                     /gene="aroB"
                     /locus_tag="ACGI59_002943"
     CDS             1435..2499
                     /gene="aroB"
                     /locus_tag="ACGI59_002943"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFG9740722.1"
                     /translation="MEEKLLVNLKENSYNIFIKKNILKNIGEEIKKIYFGNKIFIVTD
                     ENIAKLYLDKVKNNLSESGFEIKSLILKSGEETKSFNTLPKIYDSLLDFKLTRKDLII
                     TLGGGVIGDLGGFAAATFLRGISFIQIPTSLLAQVDSSVGGKVGVDLERGKNLVGSFY
                     QPKAVFIDPNVLLSLPEKFYRDGMAEVIKYGCIKDKTFFNKLLSLKNREEVMQHIEYI
                     IHKCCFIKKTVVEADEKDFGDRMLLNFGHTLGHAIEKYYNFTGYTHGEAVAIGMYKIT
                     LESEKIGITKKGTSELIKSILMNYNLPYEIELRDNKSIVDAISLDKKNLGDTLKIILL
                     KDIGESIIYNTTSKFFQEVR"
     gene            2499..3812
                     /gene="aroA"
                     /locus_tag="ACGI59_002944"
     CDS             2499..3812
                     /gene="aroA"
                     /locus_tag="ACGI59_002944"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFG9740723.1"
                     /translation="MGSLKIYPSKLSGDVKIPPSKSMAHRAVICSSLSNGKSRISNID
                     FSDDIIATIRAMTSLGAIIEKKEDILEISGIFSKEGILNRENQLNQPKLTIDCNESGS
                     TLRFLVPISLTFDGVKRFIGRGNLGKRPLDTYYEIFDRQNIKYSYKENQLDLIISGKL
                     KPDEFRVKGNISSQFITGLLFILPTLESDSKIIITTELESKGYLDLTLSTIKDFGVEI
                     INNNYKEFIIKGNQTYKARDYKVEGDYSQGAFYLSADAIGEDISILDLKEDSLQGDSE
                     VVEILSRMGMEILREGNKIKGITNGLNGTLIDASQCPDIIPVLSVVASLSIGRTTIIN
                     AGRLRIKECDRLHAINVELSKLGANIEEKEDSLIIEGVSKLNGGVEVWSHKDHRIAMT
                     LAIASCRCDKPIILKDFECVSKSYPHFFKDFKMLGGRIDEWNMGK"
     gene            3790..4869
                     /gene="aroC"
                     /locus_tag="ACGI59_002945"
     CDS             3790..4869
                     /gene="aroC"
                     /locus_tag="ACGI59_002945"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFG9740724.1"
                     /translation="MSGIWGNNLKVSIFGESHGNAIGINIDGLPSGIELDLDNIDKEM
                     KRRAPGKNSISTSRNESDIPEILSGYFNGRTTGTPLCAIIRNSDTRSKDYGELKNLMR
                     PGHADFTGNVRYSGFNDYRGGGHFSGRITAPLVFCGAICKQILSQKGIEIGAHIKKIK
                     NIEDMSFDYVNISKQQLSNLQTLELPLLDLSKEEAMKNTIIDAKNQGDSVGGIIECAV
                     VGINVGLGNPFFDSVESTLSHLLFSVPAVKGVEFGLGFELADMYGSQSNDEMYYEGNQ
                     VKSKTNNNGGIIGGITTGMPIIFKVAIKPTPSISRQQNTVNIKDKKDDILYIKGRHDP
                     CIVQRAIPVIEAVTAIGIFDLMKGR"
     gene            4872..6068
                     /gene="pheA"
                     /locus_tag="ACGI59_002946"
     CDS             4872..6068
                     /gene="pheA"
                     /locus_tag="ACGI59_002946"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HFG9740725.1"
                     /translation="MEDLTRYRNEIDNIDKELIQLFEKRMNTVLEIARHKMKNNTTIL
                     QKDREEKVLSRAVDNLDNKAYSQETVQFFNSIMEISRGLQKRLIDNDGEQKIEFSLDS
                     SNKKFDLSNVNRYKSLKDELNKKNILVGFPGKSGSFTEEALNKFFNKKISKKQFKEFE
                     DVFIALKNKEINYGIIPIENSSTGAISETYDLLRKYGFYIVGEECIKIDQNLIGIKDT
                     KLEDIKEIYSHPQGIGQCSKFLKQNSTWKLIPFNNTATSAELVRKLQDKTKAAIASKK
                     AASIYGLEIISPCINDIANNYTRFVVISNQIHIEEESNKMSVVFSVEHEAGKLYKVLG
                     YFAKNNINMTKIESRPMKNASWRYFFYIDFECSIYNAKVYDLLELIEHNTAYFKFMGV
                     YKNKTE"
     gene            6115..6924
                     /gene="aroE"
                     /locus_tag="ACGI59_002947"
     CDS             6115..6924
                     /gene="aroE"
                     /locus_tag="ACGI59_002947"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFG9740726.1"
                     /translation="MNFFGLVGEKLSHSVSPQIHKRVFEILNIESAYKNFEISKEDIS
                     KLDGAIKLLGIQGVNVTVPYKERIMKYLDFISPEAKRIGAVNTILLRENMLYGYNTDY
                     FGLDSMFKMANIDVQGKVAVILGTGGASKAALTYFIDSGIEKLYVSTRKKDDKKLLNS
                     KAILIDYEELKHIKGDIILNATPVGMYPNVGISPVSKSIIQNFDILIDLIYNPGETEF
                     LRIGNSMGKKTCDGLYMLVGQAIKSQEIWQDTKIDNSILDVIYNELKLEFL"
     gene            6936..7469
                     /locus_tag="ACGI59_002948"
     CDS             6936..7469
                     /locus_tag="ACGI59_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HFG9740727.1"
                     /translation="MINKTKEKLILIGMPGSGKTTIGKLLAKEYNCSFCDMDDYIIQI
                     SQKSIAELFSEGEDIFRNYETQACRELSISDKTVISTGGGVIKKDVNMEILKETGIII
                     FIDRPIQKILEDININSRPLLKNGKDRLYNLYNERINLYKKFSDIEILNDKSLNNAVY
                     NITNAVSENFKFDFKEK"
     gene            7490..8329
                     /locus_tag="ACGI59_002949"
     CDS             7490..8329
                     /locus_tag="ACGI59_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HFG9740728.1"
                     /translation="MNIVIVGLGVIGGSFAKALKKAGYENVFGVDVDLETLKKAEKAK
                     IIKKGCTTGKELFKKADLIILSIYPRLVVDFLNNNKNFFKKGTIITDTTGIKETLIND
                     VLQIIPDDIDFIFGHPMAGREKKGIDFASEQVFNGANYIITPTGRNNIKNLELVENLI
                     LEIGFKRVKKLTSQKHDEIIAFTSQLPHVMAVALINSDEEGRDTGKFIGDSYRDLTRI
                     ANMNEDLWSELFLGNRDNLLKVIENFESEVNLVKEAIFNNDKNKLIEYFKKSSIRREA
                     LEK"
     gene            8597..10078
                     /locus_tag="ACGI59_002950"
     CDS             8597..10078
                     /locus_tag="ACGI59_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HFG9740729.1"
                     /translation="MKKNRLIAKNISIAFIVLFFFSVFTFFYVGNINRVLEYETNDII
                     TVTIAGWIILSFLFLGIIIYILYSKANSQKTIEKVAYTDFVTGYSNWRKFELDVTNLL
                     KKTSQNNKYAMVIFDIDKFKAINDIYGHKKGNLILKDIADTLNELTDINETFARVSAD
                     NFNILLTYNKKEDIINIIKKIMANNELVNLSFGIYEIKDKDLSVSVYSDRASLAKSSI
                     KNNSDVNFAFFNDKLREKLLFEDKIEKEMEYALESGQFVMYLQPKYNIKLDKFCGSEA
                     LVRWQYTEKEVIYPGDFIPIFEKNGFIRKIDMYILEQACKEIRSLFDKGISPLPISVN
                     FSRVDFFKKDFIENIVNICDRYKIPYSLIEIEITESSMFGDTDTLFNVSRNLQDIGFI
                     VAMDDFGSGYSSVNMLKNIPLNVIKLDRGFFVDDKDVDKSQIVIKSIVSLIKQLGIRV
                     VAEGIETRSQIEMLKKANCDIVQGYYFSKPLPIKEFEKLVYKI"
     gene            10111..13545
                     /locus_tag="ACGI59_002951"
     CDS             10111..13545
                     /locus_tag="ACGI59_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HFG9740730.1"
                     /translation="MIRAKSLKNVLSIVLIFLFTIISFSDFAFKTNVQEEENSKINLR
                     EISLQSSNAIKIKLDSSLSFIKSVANMVSYFNTIDNSKIVPLLANISKESDFQRISIV
                     SPDGISHTSDGKIINVSKYQYFKKAIKGESNISEVVKSPIDGKDTFVIATPVFNKNNK
                     IIGVLYGSYYNDKLAEFIDITSFNSEGYIDIFESSGQFVLKSSHTNKLIKSSSNIYDI
                     FKKASFDDGYSYKKIYEDTKNNKSGFTSYEYKGENRYATYTPIGVNDWYVISVIPQYT
                     VRRNIDKINQLAFSLIFKVFLLFIMLIIYIIYKNNKSQKDILKANKEISSITNSIPGG
                     VQKCTLGETCNINFLSDGFIELFGYTREEIKTLFNNDFYSLIHKDDVERIKKEIFKQL
                     YKNKTVELEYRIITKNGNTVWVLDKGELITEPDGKEYFCCVTVDITRTKQLEQEMEQK
                     NLELEMISENVLGGIIITEFNDDFTIKYANSGYLSMIGYTREQLENELKYKACNLVFY
                     EDKKKALESIQHQLNTKGIAVYQHRIRRRNGNIIWVSIKGRLIVDDGIEKGIWILNDI
                     TDLKNAEQKLKINEERFRIALKQTSNTIFEYDIKTKSLLNTSKELDKYNIYKITKNVP
                     YSLVESGVIHKDFSDDFINMYENVINGAKKASCVVKMKLPDENYIWNKVTLTTIFDED
                     GNPVRAIGIQEDISKQKEAEIRYNQEGKYHSAILSEAMITYEINVSKDTFIKGNESWQ
                     KPFGIKPDNNYSQMVKTIANSIVHKDNKETFLNTFLCENLLDAYKNNVTEIKLEYRRM
                     NQENKMIWVICTMHLIKDSITNDIKALAYVKDIDKQKNEEIRLKLKAERDTLTGLYNK
                     GTTEKLINDFLNSEEASLGMHAFLIVDIDNFKSVNDNLGHVFGDKVLFEVSKNLKPIF
                     RKDDIVGRIGGDEFIVFLKNIQSFENIKKKAEKICDIFRNSYTENKKTYKISGTVGIS
                     FVPEHGTVFEELYKKADIALYYAKSKGKDKYALYNDCIYQNELENNISDNNIKLADKC
                     EKSKPVLKEILDSIDNYIYIVNPNTYELLFVNNKVSRLDLEIKLGETCYKSLLKSDRP
                     CNNCPLNGLEENKHSKFKIETNTFGKNISIASWINCIDGEYNCLIECIDSSLYKNK"
     gene            13563..14309
                     /locus_tag="ACGI59_002952"
     CDS             13563..14309
                     /locus_tag="ACGI59_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFG9740731.1"
                     /translation="MLNSITLILGIIFTIYYFYLKIVFGGISFSEIFLVVGIVLIIYQ
                     IFKKKIKEKKVFYRVLKIIISIFLIVFVVTESLIIFYPKNSLESKSDYLLILGASVKK
                     TTPSTTLKGRLDTALKYLKINDNCYVVVSGGKGSGEKITEAKAMKDYLIKNGIDKNRI
                     IEEDKSTNTYENFKYSKLKIEEHSQGKLSDLKVKIVTTDFHVLRSKILAYRNGYKNTS
                     FYASKSKLSFVPTYYTREFFALWKTIIFDR"
     gene            complement(14258..14566)
                     /locus_tag="ACGI59_002953"
     CDS             complement(14258..14566)
                     /locus_tag="ACGI59_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HFG9740732.1"
                     /translation="MNFFKENSTFYFATVEDNKPRVRPFGFIMDYEGKLYFGIVKYKT
                     SYKQLLVNPNVEISTTSKVGKWIRIKGIEKTRYEFISSLFIYYLSKMIVFHNAKNSLV
                     "
BASE COUNT         6071 a         1445 c         2451 g         4702 t
ORIGIN      
        1 ataaaaaaat attttaaatt gaaaatattt atagaaaata ttgacaatta tattttcttt
       61 gttataatta ttaaacaaga agaataataa attataaaaa tggaggataa tactatgaaa
      121 ggaatattaa aagaactaac agcaaaactt tatagctttt tttactttat gttctggggc
      181 ttttatttta gcgctggcta tttcttttac aaaaatatat gtacttcctt tctggtgagg
      241 ccttatttta gtgattttga gcataagtaa attttataaa aatgattatt ttatatattt
      301 atataagatt tagtgcttga tagattattt tagagaactc attagtgagt tctctttttt
      361 attttaaata taaaattata atattgggag gtagtaaaaa tgatagtagt attaaaaatg
      421 ggagcagata aaaatgaagt taaaaaatta attgaggcta ttgggagaga aggagtagaa
      481 gttaatccca tagatggaac agaattaact gttttaggtt tagttggaga tactagtaaa
      541 atagatgcca aaagaataga agcaaataag gtagttgaaa aagttatgca tgtggtagag
      601 ccttttaaaa aggccaatag aaaatttcat ccagaacctt caatcataaa tgttaatgga
      661 atggaaattg gttcaaagaa aattgctatg atagctgggc cttgttctgt tgaaacagaa
      721 gaccaaatag tatctatagc taaagatgtg aaaaaatcag gtgcaggatt tttgagaggt
      781 ggagcattca aaccaagaac atcaccatac gctttccaag gattgaaata tgatggattg
      841 gatttattaa aaaaagcaaa agaaaaaaca gggcttccta tagttacaga aataatgtca
      901 acacaagata tagatatatt tgaagaaaat gtagatgtaa ttcaagttgg agctagaaac
      961 atgcaaaact ttgatttatt aaaagaatta ggtaaaacaa ataaaacaat acttttaaaa
     1021 agaggattat ctgctacaat tgaagagtgg ctaatgtcag cagagtatat aatggctgga
     1081 ggaaatgaaa atgtagttct ttgtgaaaga ggtataagaa cttttgaaac atatactaga
     1141 aatactttgg atttaagtgc aattttagct gtgaagaaac ttagtcattt accagttata
     1201 gttgacccaa gtcatgcagc aggtaaatca tggatggttg actctttatc taaggcagca
     1261 attgcagttg gagcagatgg tctaataata gaagttcata atgacccagc acatgcactt
     1321 tgtgatggaa aacaatcgat taagccaaat gaatatgatg aattgataag tgaattaaag
     1381 actatcgcat cagcagttgg tagagaaata taatctataa aaggatggtt aaacgtggaa
     1441 gaaaaactat tagtaaattt aaaagaaaat agctacaata tatttattaa aaaaaatata
     1501 ctaaagaata taggagaaga aataaaaaaa atatattttg gaaataagat ttttattgta
     1561 actgatgaaa atatagcaaa attatacttg gataaagtaa aaaataattt aagtgaatct
     1621 ggatttgaaa ttaaatcttt aatattaaaa tctggtgaag aaacaaaatc atttaatact
     1681 cttcctaaaa tatacgatag tttacttgac tttaagttaa ctagaaaaga tttgattatt
     1741 actttaggag gaggggtaat aggtgattta ggtggatttg cagcagcaac ttttttaaga
     1801 ggtataagtt ttattcaaat tccaacatct ttattagcac aagttgatag ttctgtaggt
     1861 ggaaaagttg gagttgacct tgagagaggc aaaaacttag tgggaagctt ttaccaacca
     1921 aaggctgtgt ttatcgaccc aaatgtatta ttatcacttc cagaaaaatt ctatagagat
     1981 ggaatggcag aagttataaa atatggatgt ataaaagaca aaactttttt caataaactt
     2041 ttaagtttaa aaaatagaga agaagtaatg caacatatag aatacattat acataaatgt
     2101 tgctttatta aaaaaactgt agttgaagct gatgaaaaag actttggaga tagaatgctt
     2161 ctaaactttg gccatacatt aggtcatgct attgagaaat actacaattt tacaggttat
     2221 acccatggag aagctgttgc cataggaatg tataagataa ctttagaaag tgaaaagata
     2281 ggaattacaa aaaaaggtac atcagaactt ataaaatcaa ttttaatgaa ttataatttg
     2341 ccttatgaga ttgaattaag agataataaa tctatagtag atgctatatc tcttgataaa
     2401 aaaaatctag gtgatacatt aaaaataata cttttaaaag atattggaga gagtattata
     2461 tataatacta cttctaaatt ttttcaggag gtaagataat gggaagtcta aaaatatatc
     2521 caagtaaatt atctggagat gtaaaaatac caccatcaaa aagtatggca catagggcag
     2581 ttatttgttc atctctaagc aatggaaaaa gtagaatttc taatatagat ttttctgatg
     2641 atataatagc tactataaga gcaatgacat cattaggagc tattatagaa aagaaagaag
     2701 atattttaga aatttcggga attttttcta aagaaggtat tttaaataga gagaatcagt
     2761 tgaatcaacc taaattgaca atagattgta atgaatcagg ctctacattg agatttttag
     2821 tacctatatc tttaacattt gatggtgtaa aaaggtttat aggtagaggt aatcttggaa
     2881 aaagaccact tgatacctat tatgagatat ttgacagaca gaatataaaa tatagttaca
     2941 aagaaaatca gttagatttg attatatcag gtaagcttaa accagatgaa tttagagtaa
     3001 aaggaaatat aagttctcag tttatcacag gtttactgtt tatattgcct acgctagaaa
     3061 gtgattcaaa gataataatt actactgagc tagaatctaa aggatattta gatttaactc
     3121 tttcaacgat aaaagatttt ggggtagaaa ttataaacaa taactataaa gaatttatta
     3181 taaaaggaaa tcaaacgtat aaagcgagag attataaagt agaaggagat tattctcaag
     3241 gagcatttta tttatcagca gatgctatag gagaagatat tagcatatta gaccttaaag
     3301 aagattctct tcaaggggat agtgaagtag ttgaaatttt atctaggatg ggaatggaaa
     3361 tattaagaga aggcaacaaa ataaaaggaa taacaaatgg tttaaatggt actttgatag
     3421 atgcatctca atgcccagat ataatacctg tattatctgt agtagcatcc ttaagtatag
     3481 ggaggactac aataattaat gctggtaggc ttagaattaa agaatgtgat agacttcatg
     3541 caataaatgt tgagttaagt aaacttggag caaatataga ggagaaagag gatagtttga
     3601 taatcgaagg agtaagcaag ttaaatggtg gagtagaggt gtggagtcac aaagaccata
     3661 gaattgctat gacacttgca attgcttctt gtagatgtga taaaccaata atattaaaag
     3721 attttgagtg tgtatcaaaa tcttatccac atttttttaa agattttaag atgttaggag
     3781 gtcgaattga tgagtggaat atggggaaat aatttaaaag tatcaatttt tggagaatct
     3841 catggaaatg ctataggtat aaatatagat ggtcttcctt cggggattga acttgacctt
     3901 gataatattg ataaagaaat gaagagaaga gcacctggaa aaaattctat atctacttca
     3961 agaaatgaaa gtgatatacc agaaattcta agtggatatt ttaatggaag aactacagga
     4021 acgccattat gtgcaatcat aagaaatagt gatactcgtt caaaagatta tggagagctt
     4081 aaaaatttaa tgagaccagg acatgcagat ttcacaggta atgtgagata ttctggattt
     4141 aatgactata gagggggagg tcatttttca ggtaggataa cagctccatt agtattttgt
     4201 ggggctatat gtaaacaaat tttatcacaa aaaggaatag agataggagc acacataaaa
     4261 aaaattaaaa atatagaaga tatgagtttt gattatgtaa atatttcaaa acaacagtta
     4321 tcaaatttgc aaactttgga actaccttta cttgatttat caaaggaaga agccatgaaa
     4381 aatacgatta tagatgcaaa aaatcaagga gattcagttg gtggtataat tgagtgtgca
     4441 gtagtaggta ttaatgtagg gcttggaaat ccattttttg actctgttga atctacttta
     4501 tcacatttat tgttttctgt tcctgctgta aaaggtgtag agtttggttt aggttttgaa
     4561 cttgcagata tgtatgggtc tcaaagtaat gatgaaatgt attatgaagg aaatcaagtt
     4621 aagagcaaaa caaataacaa tggaggaatt ataggaggga ttactactgg aatgccaata
     4681 attttcaaag tggcaattaa gccaactcca tcaattagca gacaacaaaa cacagttaat
     4741 ataaaagata aaaaggacga tattttatat attaaaggta gacatgaccc ttgtatagtt
     4801 caaagggcta tacctgtaat agaagcagta acagccattg gaatttttga tttgatgaaa
     4861 ggacgataat aatggaggat ttaacgagat atagaaatga gattgacaat atagataaag
     4921 agcttattca attatttgaa aagagaatga atacagttct tgaaattgcc agacataaaa
     4981 tgaaaaataa cactactata cttcaaaaag atagagaaga aaaagtacta agtagagctg
     5041 tagataattt agataataaa gcatattcac aagaaacggt acagtttttt aattctataa
     5101 tggaaattag tagaggatta caaaaaagat tgatagataa tgatggtgag cagaaaatcg
     5161 agtttagttt agattcatca aataagaaat ttgatttgtc aaatgtaaat agatataagt
     5221 ctttaaaaga tgaattaaat aaaaaaaata tcctagtagg atttccagga aaatcaggtt
     5281 catttacaga agaagcacta aataaattct tcaacaaaaa aatatcaaaa aaacaattta
     5341 aagaatttga agatgttttc atagcactta aaaataaaga aataaactat ggaataatcc
     5401 ccatagaaaa ttcatctaca ggagctatct ctgaaactta tgaccttttg agaaagtacg
     5461 gtttttatat agttggagaa gaatgtataa aaatagacca aaatttaata gggataaaag
     5521 acacaaagct tgaagatata aaagaaatat attctcatcc acaaggtata ggacaatgta
     5581 gtaaattttt aaaacaaaat agtacatgga aattgatacc atttaataac acagcaacaa
     5641 gtgcagaact tgtaagaaaa cttcaagata aaacaaaagc agcaattgca agcaaaaaag
     5701 ctgcaagtat ttatggatta gaaataataa gtccttgtat aaatgatatt gcaaacaact
     5761 atacaagatt tgtagtaatc agtaatcaaa tacacattga agaagaatca aacaaaatga
     5821 gtgtagtatt ttctgttgaa catgaagcag gaaaacttta taaagtactt ggatactttg
     5881 ctaaaaataa tataaatatg acaaaaatag aatcaagacc tatgaaaaat gcctcatgga
     5941 gatatttctt ttatatagat tttgagtgtt ctatatacaa tgctaaagtg tacgatttat
     6001 tagaattgat agaacataat acagcatatt ttaaatttat gggagtatat aaaaataaaa
     6061 ctgaatagat aagataaatt tttatttaat ttataattat taggaggtgt aagtatgaat
     6121 tttttcggtc tagtaggtga aaaactctct cacagtgttt cacctcaaat ccacaagaga
     6181 gtattcgaga tattgaatat tgaaagtgct tataaaaact ttgaaatttc aaaagaagat
     6241 atatctaagc tagacggtgc tataaaatta ttaggaatac aaggggtaaa tgttactgta
     6301 ccatataagg agcgtattat gaaatatctt gattttatat caccagaggc aaaacgtata
     6361 ggagcagtta atacaatttt acttagagaa aatatgttat atggttacaa tacagattac
     6421 tttggtcttg atagtatgtt taaaatggct aatattgatg ttcaaggaaa agtagcagtt
     6481 atattaggaa ctggaggtgc ttctaaagct gctcttacat attttattga ttctggtata
     6541 gaaaagcttt atgtttcaac tagaaaaaag gatgataaaa aattattaaa tagtaaagct
     6601 atattaatag attatgaaga acttaaacat ataaaaggag atataatatt aaatgctaca
     6661 ccagtaggta tgtaccctaa tgtaggaatt tcaccagtgt caaaatctat tatacaaaat
     6721 tttgatatat taatagattt aatttacaat cctggagaaa cagagttttt aagaattggt
     6781 aatagtatgg gtaaaaaaac ttgtgatgga ttatatatgc tggtggggca agcaataaaa
     6841 tctcaggaaa tttggcaaga tactaaaata gataatagta ttttagatgt tatatataat
     6901 gaactaaaat tagaattttt gtaagggaga ttgttatgat aaataaaaca aaagaaaaat
     6961 tgatattaat aggtatgcca ggtagtggaa aaactacaat tggtaaactt ttggccaaag
     7021 aatataattg tagtttctgt gatatggatg attatataat tcaaatatct caaaaaagta
     7081 tagcagagtt gttttcagaa ggagaagata tctttaggaa ctatgagact caagcatgta
     7141 gagaactttc tataagtgat aaaacagtga tatctacagg tggaggtgta ataaaaaagg
     7201 atgttaatat ggaaatacta aaagaaacag gtataataat ttttattgat agacctatac
     7261 aaaagatttt ggaagatata aatattaatt caagaccatt attaaaaaat ggaaaagata
     7321 gattgtataa tctttataat gagagaataa atctttataa aaaattttca gatatagaga
     7381 ttttaaatga taaatcatta aataatgctg tatataatat tacaaatgca gtcagtgaaa
     7441 attttaaatt tgactttaaa gaaaaataat ctagggggaa ataaaaataa tgaatattgt
     7501 tattgtagga ctaggagtta taggaggctc ttttgctaaa gcattaaaaa aagctggata
     7561 tgaaaatgta tttggagtag atgtagattt agaaacttta aaaaaagcag aaaaagcaaa
     7621 aatcataaaa aaaggatgta ctacaggtaa agagttattt aaaaaagctg atttaattat
     7681 attatctata tatcctagat tagttgtaga ttttctaaat aataataaaa acttttttaa
     7741 aaaagggact ataattactg atactacagg aataaaagaa acacttatca atgatgtgtt
     7801 gcaaattatt ccagatgata tagattttat atttggacat ccaatggcag gtagggaaaa
     7861 aaagggtatt gattttgcaa gtgaacaagt ttttaatggc gcaaattata taattactcc
     7921 aactggcaga aacaacataa aaaatttaga gttagttgaa aatttaattt tagagattgg
     7981 atttaaaaga gtaaaaaagt taacttcaca aaaacatgat gagattatag cttttacttc
     8041 tcagttacct catgttatgg cagttgctct tataaatagt gatgaagaag gtagagatac
     8101 gggtaaattt attggagata gttatagaga tttaactaga attgcaaata tgaatgaaga
     8161 cttatggagt gaactttttt taggaaatag agataattta ctaaaagtta tagaaaactt
     8221 tgaatcagaa gtaaatttag taaaagaagc tatatttaac aatgataaaa ataaattaat
     8281 agagtatttt aaaaaatcat caataagaag agaagcttta gaaaaataaa tattaatatt
     8341 taagctatat atagatgggt ataaatataa gcgatagtgt atttaattgt attaataaat
     8401 atggattcaa tataattaaa tatgctattg atatatttgt acccttaact attgttatct
     8461 tttaaatgat aatgatattt atttaaatac tctttattaa caatttataa aacttaattg
     8521 taaaaaatac ttattaatag taaaataaag tgaatcaatt atattataat tgctaaagag
     8581 agtgaggtgt agctatttga aaaaaaatag attaatagca aagaatatat caatagcatt
     8641 tatagtacta ttctttttta gtgtatttac atttttttat gtaggtaata ttaatagagt
     8701 gttagaatat gaaacaaatg acataataac agttacaatt gctggatgga ttatactttc
     8761 atttttattt ttaggtatta taatttacat attatattca aaagcaaata gtcaaaaaac
     8821 tatagaaaaa gtagcatata cggattttgt aacaggttat tccaactgga gaaagtttga
     8881 attagatgtt acaaatttat taaagaaaac atcacaaaat aataaatatg caatggtcat
     8941 attcgatatt gataaattta aagcaattaa tgatatatat ggccataaaa agggaaattt
     9001 aatattaaaa gatattgcag atactttaaa tgaattgact gatatcaatg aaacttttgc
     9061 tcgtgtaagt gctgataatt tcaatatatt attaacatat aacaaaaaag aggatataat
     9121 aaatattatc aaaaagatta tggcaaataa tgaacttgta aacttatctt ttggaattta
     9181 tgaaatcaaa gataaggatt tatctgtaag tgtatatagt gatagagctt cacttgcaaa
     9241 atcatcaata aaaaataata gtgatgtaaa ttttgcattt tttaatgaca aattaagaga
     9301 gaaattatta tttgaagata agatagaaaa agaaatggaa tatgctcttg aaagtgggca
     9361 attcgtaatg tatttacaac caaaatacaa tattaaatta gataaatttt gtggaagtga
     9421 agccctagtc agatggcaat atacagaaaa agaggtcata tatccaggtg attttattcc
     9481 tatatttgaa aaaaatggat ttattagaaa gattgatatg tacatattag aacaagcttg
     9541 taaagaaatc cggtctttat ttgataaagg aatttcacct ttacctatat ctgtaaactt
     9601 ctcaagagtt gattttttta aaaaagattt tattgaaaac atagtaaata tatgtgatag
     9661 atataaaata ccatatagtt taatagaaat tgaaattact gaatctagta tgtttggaga
     9721 tactgatact ctatttaatg ttagtagaaa cctacaggat attggcttta tagttgcaat
     9781 ggatgatttt ggctctggat attcttctgt aaatatgctt aaaaatatac cattaaatgt
     9841 aataaaatta gatagaggat tttttgttga tgataaagat gttgataaga gtcaaattgt
     9901 tataaaaagc atagtttctc tcattaagca attaggaata agggttgttg ctgaaggaat
     9961 tgaaacaaga agtcaaatag aaatgttaaa aaaagcaaat tgtgatattg ttcaaggata
    10021 ttatttttca aaaccattac caattaaaga atttgaaaaa ttagtctata aaatatagtc
    10081 agcgagtctt aaatgggagg tggtgattaa gtgataagag caaaatcgtt aaaaaatgta
    10141 ctgagtattg ttttaatatt tttatttaca ataatttctt tttctgattt tgcatttaaa
    10201 actaatgtcc aggaagaaga aaatagtaaa attaatttaa gagaaatatc cctacaaagt
    10261 tccaatgcta taaaaataaa attagatagt tctctatcct ttattaaaag tgtggcaaat
    10321 atggtttctt attttaatac tatagataac tcaaaaatag ttccattatt agctaatata
    10381 tcaaaagaat ctgactttca aagaatctct attgtatctc ctgatggaat aagtcatacc
    10441 agtgatggca aaattatcaa tgtgtcaaag tatcagtatt ttaagaaagc tattaaagga
    10501 gaaagtaata tatctgaagt tgtgaaatct ccaattgatg gaaaagatac ttttgttata
    10561 gctactcctg tatttaataa gaataacaaa ataattggtg ttttatacgg ttcttactac
    10621 aatgataaat tagctgaatt tatagatata acaagtttta atagtgaagg gtacatagat
    10681 atatttgaat ctagtggtca atttgtttta aaatcttcac atacaaacaa actgattaag
    10741 tcaagtagta acatatatga tatatttaaa aaagcttctt ttgatgatgg ttatagctat
    10801 aaaaaaatat atgaagatac aaaaaataac aagagtggct ttacctcata tgaatacaaa
    10861 ggagaaaatc gttatgctac ttatacaccc attggtgtaa atgactggta tgtaatttct
    10921 gtgataccac aatatactgt aagaagaaac attgataaaa taaatcaatt agcattttct
    10981 cttattttca aggtattctt attgtttatt atgcttataa tatatattat ttataaaaac
    11041 aataaatctc agaaagatat actaaaagct aacaaagaaa tctcatctat tacaaatagc
    11101 attcctggag gagtacaaaa gtgtaccctg ggtgaaacat gcaatattaa ttttttaagt
    11161 gatggattta tagagttgtt tggttataca agagaagaaa taaaaacatt gtttaataat
    11221 gatttttata gtttaataca taaagatgat gttgaaagaa taaaaaaaga aatttttaaa
    11281 caactttaca aaaataaaac tgttgaatta gaatatagga ttataacaaa aaatggtaat
    11341 acagtatggg ttttagataa aggggagctt ataacagaac ctgatggaaa agaatatttt
    11401 tgttgtgtaa ctgtagatat tactagaaca aaacaattag aacaagagat ggaacaaaaa
    11461 aatctagaac ttgaaatgat atcagaaaat gttttaggtg gtataattat aacagaattt
    11521 aatgatgatt ttactataaa atacgctaac tctggttatc tttcaatgat tggatataca
    11581 cgtgaacaat tagaaaatga acttaaatac aaagcttgta atcttgtttt ttatgaggat
    11641 aaaaagaagg ctcttgaaag tatacaacat caattaaaca caaaaggaat agctgtatat
    11701 caacatagaa taagaagaag aaatggtaat attatttggg tttctataaa aggaaggctt
    11761 attgttgatg atggtattga aaaaggtatt tggattctaa atgatataac agatttaaaa
    11821 aatgcagaac aaaaacttaa aataaatgaa gaaagattta gaattgcatt aaagcaaaca
    11881 tcaaacacta tatttgagta tgatataaaa acaaagtcac ttttaaatac tagtaaagag
    11941 cttgataaat ataatattta taaaattact aaaaacgttc catattctct agtagaatca
    12001 ggagttattc ataaagattt tagtgatgat tttataaaca tgtatgaaaa tgtaataaat
    12061 ggagcaaaaa aagcaagttg tgtagttaag atgaaacttc cagatgaaaa ttatatatgg
    12121 aataaagtta ccttgactac tatttttgat gaagatggta atcctgtaag agcaattggt
    12181 atacaggaag atataagcaa acaaaaggaa gcagagataa gatacaatca agaaggtaag
    12241 tatcattcag ctatattgtc ggaagcaatg ataacttatg aaataaatgt atcaaaggat
    12301 acttttatca aaggaaatga aagttggcaa aaaccatttg gaattaagcc tgacaataac
    12361 tattctcaaa tggtaaaaac tatagcaaat agtattgtac ataaagacaa taaagaaaca
    12421 tttttaaata catttttatg tgaaaactta ttggatgcat ataaaaataa tgtgactgaa
    12481 ataaaattag aatatcgaag aatgaatcaa gaaaataaaa tgatttgggt catttgtaca
    12541 atgcacttaa taaaagattc tattacgaac gatataaaag cacttgctta tgttaaagat
    12601 attgacaagc aaaaaaatga agaaattaga ttaaaattaa aagctgaaag agatactctt
    12661 acaggattat ataataaagg tactacagaa aaattgatta atgatttctt gaactctgag
    12721 gaagcaagct taggaatgca tgcattttta attgttgata tagataattt taaatctgtg
    12781 aatgataatt taggacatgt atttggagat aaagtattat ttgaagtttc taaaaaccta
    12841 aaacctatat ttagaaaaga tgatatagta ggtagaattg gtggagatga atttatagta
    12901 ttcttaaaaa atattcagtc ttttgagaat atcaaaaaga aagcagaaaa aatatgcgat
    12961 atatttagaa attcttatac agaaaataaa aagacatata aaatatctgg aacagttgga
    13021 atttcatttg taccagaaca tggaactgta tttgaagaac tttataaaaa agcagacata
    13081 gcactttatt atgcaaagag caaaggaaaa gataagtatg cactttataa tgattgtatc
    13141 taccaaaatg aacttgaaaa taatataagt gataataata taaaattagc agataaatgt
    13201 gagaaatcca aaccagtact taaagaaatt cttgattcta ttgataatta tatctatata
    13261 gtgaatccaa atacttatga acttcttttt gtcaataata aggtgtctcg tttggattta
    13321 gagataaaat taggagaaac ctgttataaa tcactattaa aatctgatag accatgtaat
    13381 aattgccctc ttaacggatt agaagaaaat aagcattcaa aattcaaaat agagacaaat
    13441 acatttggta aaaatatcag tatagcaagt tggattaatt gcatagatgg agaatacaac
    13501 tgtcttatag agtgtataga ttcttcttta tacaaaaata aataaaaaag gactatgaag
    13561 atatgttaaa tagtataact ttgatacttg gtataatatt tacaatttac tatttttatc
    13621 tcaaaatagt ttttggagga atttcattta gtgagatttt cttagttgta ggaattgtac
    13681 taattatcta tcaaatattt aagaaaaaaa ttaaagaaaa gaaggttttt tatagggtgt
    13741 taaaaataat aatcagcatt tttttaatag tatttgtagt tactgagagt ctaattattt
    13801 tttatcccaa aaatagttta gaatcaaaaa gtgattatct cttaatttta ggagcttctg
    13861 taaaaaagac tacaccaagt acaaccctga agggtaggtt agacactgca ttgaaatacc
    13921 taaaaattaa tgataattgt tatgtggtag tatctggtgg taaaggaagt ggagaaaaaa
    13981 taactgaggc aaaagctatg aaagattatc ttataaaaaa tggtatagat aaaaatagga
    14041 taatagaaga ggataaatcc acgaatactt atgagaattt taaatattca aaattgaaga
    14101 tagaagaaca tagtcaagga aaactaagcg atttaaaagt aaaaatagta acaacagatt
    14161 ttcatgttct cagaagtaaa atattagcat atagaaatgg atataaaaat acgtcttttt
    14221 atgctagtaa atcaaaactt tcatttgtac caacttacta tacaagagaa ttttttgcat
    14281 tatggaaaac aatcattttt gatagataat aaataaaaag actagatata aactcatatc
    14341 tagtcttttc tattccctta attctaatcc attttccaac tttacttgtt gtagaaattt
    14401 caacattggg atttacaagt aattgcttat aagaagtttt atattttacg attccaaaat
    14461 ataattttcc ttcataatcc attataaatc cgaatggtct tactcttggt ttattatctt
    14521 caactgtagc aaaatagaat gttgagtttt ctttaaaaaa attcattatt ttttcatgat
    14581 taaatcctct ttgtatctta tactaatata atgaatataa ataaaatatt cataatacta
    14641 atataaatcg atataaaaac aattacaat
//
LOCUS       DAYAPA010000109        14604 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.110, whole genome shotgun sequence.
ACCESSION   DAYAPA010000109 DAYAPA010000000
VERSION     DAYAPA010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14604
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.110"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(2..253)
                     /locus_tag="ACGI59_002954"
     CDS             complement(2..253)
                     /locus_tag="ACGI59_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9740733.1"
                     /translation="MNRYTKIINMMGSYYTKDFEKEKKNVIKVREVKEDTVRKFFLQG
                     DCEVLVVFEDTGKEILIDDFSPEEDIKKYLGAKFINKKR"
     gene            342..1073
                     /locus_tag="ACGI59_002955"
     CDS             342..1073
                     /locus_tag="ACGI59_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFG9740734.1"
                     /translation="MTSIKNKDILNCIDYSIKNKLFDKLNDVYKSLPVGNCSGCGNCC
                     MESVGINLIEFLNIFCYLEDRVDLKKSCISKILDYYFEEYTRKNPCPFKDTNNRCLIY
                     EVRPLNCRMFGHWKKEDYNKNLDNVIEKNNNYRELMKKQYGFEINDEVVNYRIDYCDR
                     FIPNKDYLSKSKRLSFFDELMILDSNIYSSGNIDIDFRDRGIVEYFIESLLYRNLAYN
                     VKIRISKEYKIRKRTIDRIKRIVLV"
     gene            1132..2043
                     /locus_tag="ACGI59_002956"
     CDS             1132..2043
                     /locus_tag="ACGI59_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFG9740735.1"
                     /translation="MNLTKVKGNTFFIKGGTNTGIYVFEDNTALMIDPGLCGSRPRRV
                     NKVLDENNIQVRYIINTHGHDDHYGACNQFKEYYKDVCIMSSEYDKLYIENPELFSKY
                     IVGGKSNVFMDNILKNKMLDDIKIDKSIEAGKIELNNEMFDIIEFQGHTPGSIAVLTK
                     DNVIFVGDLLIGDEILSKYDFLFLYDIQEQINSLEKLKNIDFEYLVLGHGKQVISKED
                     SYKLIEKHLNALKKYLYQIRELLVEPKSLEILLKNIINNNNLSNNYKEYHFFKSSLVS
                     VISYLVDLEEIGYILENGELLYYTKTK"
     gene            2094..2906
                     /locus_tag="ACGI59_002957"
     CDS             2094..2906
                     /locus_tag="ACGI59_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HFG9740736.1"
                     /translation="MEYEKWNEILAPYEHAVEELKIKFKNIRKEYLAKGEYSPIEFVT
                     GRTKKISSIISKLKRINAKDIETEIDDIAGIRIMCQFVEDIYAIVDLIKVRNDMTIIG
                     EKDYITNYKDSGYRSYHVIIKYPINSIAGSKEIICEIQIRTLAMNFWATIEHSLKYKY
                     DHYIPETLAERLRRASDAAFLLDQEMSEIREDIMKAQAMYQMKSIAIRDTLNRIQELY
                     DLGDTHKAMQYQRKLDRTETDKNITEILELKQEIDILLEQYKDTKVDDKVVQ"
     gene            3015..3707
                     /locus_tag="ACGI59_002958"
     CDS             3015..3707
                     /locus_tag="ACGI59_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFG9740737.1"
                     /translation="MSLYAIGDLHFSTSVNKPMNIFGSNWDGHEKKIIDNWKEVVKEE
                     DMVLVLGDTSWGINLSEAKKDLDIISKLPGQKILIKGNHDYWWTTVTSLNKLYEDMRF
                     IQTNFYEYKDYAICGGRGWICPNDVKFDETDEKVYKREEHRLRLSLESARKSGHSKII
                     VITHYPPTNDKLEESLFTKLFEEYNVEKVIYGHLHGKESFKMGLKGIRNGVEYTLASC
                     DYTEFNLIKVHD"
     gene            3794..4735
                     /locus_tag="ACGI59_002959"
     CDS             3794..4735
                     /locus_tag="ACGI59_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9740738.1"
                     /translation="MRWILVFIFLYLIPLVVIFRNYKNFKRSCIYSSIYVVLATTIMI
                     SNIYMSGLNKIKESMYYHNYISDETYDEKYVSNFKEKESTDLKQDNKKNEEISNNKNL
                     NNKQDRNIQNKESNEEGSNDIKEKNNSDSNVSVNNKSEKQEDNTKENNKYTSKEKNSE
                     DIKKIDMESIGIFKKEIYNIERAALVPMRECIPYTKDIAKNITKLSSIKKDVTNARNK
                     CKEVVKEYENMQIPELSKEKYTETLSNARDDVKKAYELREKSMESALKLINTKSPKYI
                     GKITEYLDLSDKQIESFKNRLNDLKEEINNSETNNIN"
     gene            4772..5929
                     /locus_tag="ACGI59_002960"
     CDS             4772..5929
                     /locus_tag="ACGI59_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HFG9740739.1"
                     /translation="MNKQSIGKLNMFFMGITQRNLDNRDFFIELEVIFKSGRKEFKGI
                     AIEEDNKYKFNFKGKSDLYTFNELLKNISKEAENYDGLVFKYVERGTIVVIEGDNKKV
                     NVKYLDNKEEVPKIDEFTASQIKNRDYYVKVGQANALLKEIGVLTKDGKIKNDKIRKY
                     NQIDHFVELIDSILKEIKDKDCITILDCACGKSYLSFVLNFYIKEVLKKNCYFIGIDY
                     SDVVIEASKNMAKNLGYKNMSFIKEDLTNYTPNRDVDLVISLHACDTATDMAIGLGIR
                     AKSEAIVVVPCCHKELLGQYRYEAMEPILKHGVFKARFADLITDGLRTLLLEGNGYDT
                     SVVEYISPLDTPKNLMIRAIKTKTNNDKALKEYKELKSQFGVEPTLEKLIY"
     gene            6081..6641
                     /locus_tag="ACGI59_002961"
     CDS             6081..6641
                     /locus_tag="ACGI59_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFG9740740.1"
                     /translation="MNKLNICIDIDGTITSPYHFLPYLNDIFNKNITEAECITHNWEE
                     LYGSGIRDVYAEFNNKYIHSYDEAKIVEGATEVISTLSKGHNLSFVTARHECLTDVTK
                     NWLSRQGFSDIDVYLLGSDYKVEKARELSCDIFIEDNPLNSVQLADDGVKVILLDTNY
                     NKDVKHQNIVRVSNWIDIEKIIKQGI"
     gene            6669..7160
                     /locus_tag="ACGI59_002962"
     CDS             6669..7160
                     /locus_tag="ACGI59_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9740741.1"
                     /translation="MIDLKIENRKGKIHVKSSEIKDILELRPDFEYVQDISNTINQEN
                     ILVFDCQLTGNSFDMDDLDIEEILEELGEEIDESYFSVLFEDVRAYLKDATDEIEADL
                     QDNYLFDNLRCYFDIYNINQEFTDFKFVFLVSFKDIKISSLANLAKIVSKRQLIGASK
                     FYS"
     gene            7389..7940
                     /locus_tag="ACGI59_002963"
     CDS             7389..7940
                     /locus_tag="ACGI59_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFG9740742.1"
                     /translation="MQSLNEIIAENLKKIRKEKHLSLDKIAQLSGVSKSMLSQIEKCE
                     VNPTISTLKKITNGLKISFTSLMERQESDIELIQKSDIDHFIEDNGKYISYPIFPFDS
                     KRRFEIFMIEIEEGGNLDSNAELPGTQEFITVFSGEVTIKINGEDYIVSSGNSIRFKA
                     DVSHIYRNSGKGIAKMSMVVYYI"
     gene            8026..8958
                     /locus_tag="ACGI59_002964"
     CDS             8026..8958
                     /locus_tag="ACGI59_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFG9740743.1"
                     /translation="MNNKKAVFFFCSDLKKDPVASNVIKCCEEIMDLNQTDIVIDDNF
                     VLEFKDKNNNLFHFVKTKDVISHNYKYYLPILNRHFRDYDFAGVVNWHEGENAPEHIL
                     TAHTIGDVPTGEFGNSNPRYFKNLINAIEDIKNENLLDEFTTLTEATHWSGTTYGEES
                     KLITEYSVPMLDIEIGSSSDSFNNSIAIQVLAKSLIRVFDCDEPLKTLLCVGGVHFEK
                     SFSDIIKNKEYNISIGHVLPNQWIVSGMYDDESGFEKLEKCINSIEGGIDCIVFHDKL
                     KGTYKEQCRKLGEKLNVPVFKHKILKNPKDLPIW"
     gene            9013..9876
                     /locus_tag="ACGI59_002965"
     CDS             9013..9876
                     /locus_tag="ACGI59_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFG9740744.1"
                     /translation="MILSYFALLIRVVLLAFERVVVRLLGDEEGDIYKNIASSFLFFF
                     IGGVCLLPFSIMDRVSDWSFLIPCYISSLVYSIGSVAYVTSLATGEVSLVTPINSLNS
                     LFLLLLSVIFLGEGLSLAKVAGIIIMIGGVFILKKVSSFLKNVSVIFNNLPCRLMFLY
                     IFLQSVGRVLDKAFFVQVSPILYSTILYFFVGLNLFIFLAFKKKQKIICEIFTNKKGL
                     SILSGAINGYSYLALLIALNNLELSIAEPFSQVSMIITLILAHFIFKENIKEKIPGSI
                     LILIGGWLLLL"
     gene            10123..11985
                     /locus_tag="ACGI59_002966"
     CDS             10123..11985
                     /locus_tag="ACGI59_002966"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFG9740745.1"
                     /translation="MNYKKTAIDIIEQVGGSKNILALEHCSTRLRFKLIDRSKVNQDA
                     LKKVQGVMSIVNGAQFQVVIGNSVVDVYDEILKVCPLDTNDSTVKTEEKINLGTRFIE
                     FIINIFQPLIFAIAGAGILKSLLMLLAMFNILKSDSTIYTLLVAISDATFYFLPLMVA
                     VTTANVLKCNRLVAIAAVGYLLLPATTTALSEGVELFGFTIPNIAYNAQVFPAILCVS
                     FLATMEKIFNKYSPKPIRTFFVPMMSLAITVPITLTILGPLGYNVGTIFTTIILFLYN
                     KMGFLVVGLLAAFLPFMVATGMHKALIPYSINTIGKLGYEALYMTSSLAHNISESGAC
                     FAVALRTKDETLKQTALSAGISALMGITEPALYGITLQHKRAMLGVVFSSAISGTFLG
                     LFAVKGFVIVGPGLASMSMFIDPNNGNNLIFASIGFVIAILGSFIITLIIWKEDTNTV
                     ESSIGDVVEEELEEEKEVNILVTPVEGKVIDLSKVNDELFASKTLGDGVAIIPTNGNL
                     YAPCDSEVVMLFETKHAIGLRTKNGAEILIHIGINTVSMNGDGFKTFVKTGDNVKEGD
                     LLIQFDLDKISHANLDSTVMIVNNNGADYAYKVLNQSYGNVKKGSILFDVKGGI"
     gene            11988..13454
                     /locus_tag="ACGI59_002967"
     CDS             11988..13454
                     /locus_tag="ACGI59_002967"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFG9740746.1"
                     /translation="MRKFPEGFLWGGATAANQFEGGWNLGGKGWSVSDVAKAHFDADV
                     KDYKSNNEITTKDIEEGLAHPEDEVNYPKRHGSDFYHHYKEDIALMAEMGFKTYRMSI
                     AWSRIFPNGDDKEPNEEGLQFYDDVFDELISYGIEPLVTMSHYEPPLNIVLNYDGWYS
                     RQVINMFVRYVETICERYKNKVKYWLTFNEVDSMIRHPYTTGGLVRDRFKDKNFEEVI
                     FQAMHHQFVASAIATKICHEIIPNSKVGCMLTKLTYYPYTCRPEDVLATQQKMRSIYA
                     YSDTQVFGEYPVYLLSYFKNNNIQIVKEEHDDEIMKKYPVDFISFSYYMSSCEAADTT
                     GLDITPGNTLLAVKNPYLEMSEWGWQIDSIGLRISLIELYDRYRKPLFIVENGLGAKD
                     ILTEDKKVHDQYRINYLKEHFKCMLDAIIEDGVELWGYTSWGCIDLVSESTKQMSKRY
                     GYIYVDADDYGKGTYNRYKKDSFYWYKKVIENNSIDFK"
     gene            13478..14314
                     /locus_tag="ACGI59_002968"
     CDS             13478..14314
                     /locus_tag="ACGI59_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFG9740747.1"
                     /translation="MNYIIKKVLNSSVVLVHDAKYNEFILLGKGIGYGKKTGEYICGS
                     NDNQMFVPVENTKSKQFLELMDDIPVDILKITQEIIVEAEKLLNSSFNKNLYLILADH
                     FNFAIERMRKGIKITNRVFWEIKNYYPNEFKVGMVAIYMVEERLGIQLPEEEAANIAF
                     HFANAMASDGNSYDTIKYAKVIGEIINIFVFSLNRTLDKKSMHYMRFITHIKFFVERF
                     FSDSMLSSGDDLLFNQMKRSNPKEMLIALKVRDFLEKKYGKKLTNEEIAFLVVHIARV
                     KQ"
BASE COUNT         5756 a         1420 c         2577 g         4851 t
ORIGIN      
        1 actatctttt tttatttata aattttgctc ctaaatattt tttgatatct tcttcaggag
       61 aaaagtcatc aataagtatt tctttcccag tgtcttcaaa tactacaaga acttcacagt
      121 caccttgtaa gaaaaatttt ctaactgtat cttctttaac ttctctaact tttattacat
      181 tctttttttc tttttcaaag tcttttgtgt aataacttcc catcatatta attatttttg
      241 tatatctatt cataaaatac ctccatatgc gattaaattt aaaaaatatg ttataataat
      301 tgtttgtatt aacttaataa ttataacaag gagtaataat aatgactagt ataaaaaata
      361 aagatatttt aaattgtata gattattcca ttaaaaataa gctctttgac aaactaaatg
      421 atgtatataa gtccttgcca gtaggaaatt gttctggatg tggaaattgt tgtatggagt
      481 cagtaggtat aaatttaatt gagttcttga atatattttg ttatcttgaa gatagagttg
      541 atttaaaaaa aagctgtata agtaaaatat tagattatta ttttgaggag tatacaagaa
      601 agaacccatg tccatttaaa gatactaata atagatgttt aatatatgaa gttagaccat
      661 taaattgtag gatgtttgga cattggaaaa aagaggatta taataaaaat ttagataatg
      721 ttattgagaa aaataataac tatagagagt taatgaaaaa gcaatatgga tttgaaataa
      781 atgatgaagt agtaaattac aggatagatt attgtgatag atttattcca aataaagatt
      841 atttaagtaa atctaagaga ttaagctttt ttgatgagct tatgatatta gactcaaata
      901 tttattcaag tggaaatata gatatagact ttagggatag aggtatagtt gaatatttta
      961 tagagtcatt actttataga aatttagcat ataatgtgaa aataaggata tctaaagaat
     1021 acaaaataag aaaaagaact atagatagaa taaaaaggat tgttcttgta tagttttata
     1081 ttagtaagta ataaattgta tcataaatat tgtcaaaatg aggtgaagaa ggtgaatctt
     1141 acaaaagtaa aaggaaatac cttttttata aaaggtggca cgaatacagg aatatatgta
     1201 tttgaagata acacagcact tatgatagac ccagggttat gtggttcaag acctaggaga
     1261 gtaaataaag tattagatga aaataatata caggttagat atattataaa tactcatggt
     1321 catgatgacc actatggagc ttgtaatcaa tttaaagaat attataaaga tgtgtgtata
     1381 atgtcatctg aatacgataa attatatata gaaaatccag aattgttttc aaagtatatt
     1441 gttgggggaa agagcaatgt atttatggat aatatcttaa aaaataaaat gttggatgat
     1501 ataaaaatag ataaatctat agaagctgga aaaattgaat taaacaatga aatgtttgat
     1561 ataattgaat ttcagggtca tacacctggt agtatagctg ttttgacgaa ggataatgtg
     1621 atttttgttg gcgatttatt gataggagat gaaattctat caaaatacga tttcttgttt
     1681 ctatatgata tacaagaaca aataaattca ttagaaaaat taaaaaatat agattttgag
     1741 tacttagttt tagggcatgg caagcaagtt atatcgaaag aagattctta taaattaata
     1801 gaaaaacatt tgaatgcttt aaaaaaatat ttatatcaaa taagagagtt gcttgtagaa
     1861 ccgaaatctt tagaaatttt attaaaaaac attataaata acaataattt aagcaataat
     1921 tataaagagt atcatttttt taaatcttcg ttagtatctg tcataagtta cttagttgat
     1981 ttagaagaaa tagggtatat attggaaaat ggagaattgc tttactatac aaaaacaaaa
     2041 taaattatga taaaataaag aaggtcataa aaaacaaata aagggtgagc aatatggaat
     2101 acgaaaaatg gaatgagata ctagcacctt atgaacatgc agtagaggaa ttgaaaataa
     2161 aatttaaaaa tataagaaaa gaatatttag caaaaggtga atattctccc atagagtttg
     2221 tcacaggtag aacaaaaaaa atatcttcca taatatctaa gttaaaaagg ataaatgcaa
     2281 aagatataga aactgaaatt gatgatattg ctggtataag aataatgtgc cagtttgttg
     2341 aggatatata tgctatagtt gatttgataa aagtaagaaa tgatatgact ataataggag
     2401 aaaaagatta tattacaaat tacaaagata gtggttatag aagctatcat gttataataa
     2461 aataccctat aaactccatt gcgggttcaa aggaaattat atgtgaaata caaataagaa
     2521 ctcttgcaat gaatttctgg gctacaatag aacattcatt aaaatataaa tatgaccatt
     2581 acatacctga gactcttgct gaaaggctta gaagagcatc tgatgctgcc tttcttttag
     2641 accaagaaat gagtgaaata agagaggaca taatgaaagc tcaggctatg taccaaatga
     2701 agtctatagc tattagagat actttaaata gaatacaaga gttatatgac ttgggtgata
     2761 cacataaagc tatgcagtac caaagaaaac ttgatagaac tgaaacagat aaaaatataa
     2821 ctgaaatttt ggaattaaaa caagaaatag atatattatt agagcaatat aaggatacta
     2881 aggtagatga taaagtagtt cagtaaaaac tacttgtaga aaatttgcta aatattaatg
     2941 gggctgcctc ttaatggaaa ataatttcta aaaagagaca gcctatttag tgtaattaag
     3001 aaaggaatga tattatgagt ttgtatgcta taggtgattt acatttttca acatcagtca
     3061 ataagccaat gaatatcttt ggaagtaatt gggatggtca tgaaaagaaa attatagata
     3121 attggaaaga agttgttaag gaagaagata tggtcttagt actaggagat acatcttggg
     3181 gaattaattt atccgaagct aaaaaagatt tagatataat aagcaaactt ccaggtcaga
     3241 aaatacttat aaagggaaat catgattact ggtggacaac cgttacaagt ttaaataagc
     3301 tatatgaaga catgcgtttt attcagacaa atttttatga atataaagat tatgcaattt
     3361 gtggaggtag gggctggata tgtcctaatg atgttaaatt tgatgagact gatgaaaaag
     3421 tttataaaag agaagaacat agacttagac tatcattaga atctgctcgt aaaagtggtc
     3481 actcaaaaat aattgtgatt actcattatc ctcctacgaa tgataaattg gaagaatctt
     3541 tatttacaaa gttatttgaa gaatataatg ttgaaaaggt gatttatggt cacttgcatg
     3601 gtaaagaatc ttttaaaatg ggattgaagg ggattcgaaa tggagtagaa tatacattag
     3661 catcttgtga ctatacagaa tttaatttaa taaaagttca cgattaaaaa acataaattg
     3721 gtaaatacta taagatacat gattaaaaat aaatatttga gggatttcgg ttttgagaaa
     3781 ggagacacga cttatgaggt ggatattagt ttttatattt ttatatctta ttcctttagt
     3841 agtgatattc agaaattaca agaattttaa gaggtcttgt atctacagta gtatttatgt
     3901 ggtattagct acaacaataa tgataagtaa tatttatatg agtggactta ataaaataaa
     3961 agagtctatg tattatcaca attatatttc tgatgagaca tatgatgaaa agtatgtatc
     4021 aaattttaag gaaaaagaaa gtactgattt aaaacaggat aataaaaaga atgaagaaat
     4081 atcaaataat aaaaatttaa acaacaaaca agatagaaat atacaaaata aagaaagtaa
     4141 tgaagaagga agtaatgata taaaagaaaa gaataattct gatagtaatg tttcagtgaa
     4201 taataaatca gaaaaacaag aagataatac aaaagaaaat aataaatata caagtaaaga
     4261 aaaaaatagt gaagatataa aaaaaataga tatggaaagt ataggaattt ttaagaaaga
     4321 aatttataat atagaaagag cagctttagt tccaatgaga gaatgtatac cttacactaa
     4381 ggatatagca aaaaatataa ctaaattatc tagtataaaa aaagatgtta caaatgccag
     4441 aaacaagtgt aaagaagttg taaaagaata tgaaaatatg cagattccag aactttctaa
     4501 ggagaagtat acagaaacat tgagtaatgc tagagatgac gtaaagaaag catatgagct
     4561 tagagaaaaa tctatggaaa gtgctttgaa attaataaat actaaaagcc ctaaatatat
     4621 agggaagata acagaatatc ttgatttatc agataagcag atagaaagtt ttaaaaatag
     4681 attgaacgat ttaaaagaag aaataaataa ttcagaaaca aataatatta attaataaga
     4741 tttaaagata taagttaaaa gggagattat gatgaataaa cagagtatag gaaaactaaa
     4801 tatgtttttt atggggatta ctcaaagaaa ccttgataat agggattttt ttattgagtt
     4861 agaggtgatt tttaaatctg gaagaaaaga gtttaaagga attgcaatag aggaagataa
     4921 taaatataaa tttaatttta aaggaaaatc agatttatat acgtttaatg agttgcttaa
     4981 gaatatttct aaagaggcag aaaattatga tggattagta tttaaatatg ttgagcgagg
     5041 aactattgtt gtaattgaag gagacaataa gaaggtaaat gtaaaatatt tagataataa
     5101 ggaagaagta cctaaaatag atgaatttac ggcatctcaa ataaaaaaca gagattatta
     5161 tgtaaaagta ggacaagcaa atgcactttt aaaagaaatt ggagtactta caaaagatgg
     5221 taagataaaa aatgataaaa taagaaaata caatcagata gaccattttg ttgaattgat
     5281 agatagtatt ttaaaagaaa taaaggataa agactgtata actatcttgg attgtgcttg
     5341 tggaaaatct tacttaagct ttgtcttaaa tttttatata aaagaagtat tgaagaaaaa
     5401 ctgttatttt ataggaatag attattcaga tgtagttata gaagcatcaa aaaatatggc
     5461 taaaaacttg ggttataaaa atatgtcatt tataaaagag gatttgacta attatacacc
     5521 taatagggat gttgaccttg taataagtct acatgcttgt gatacagcta cagatatggc
     5581 aataggtcta ggaattagag caaaatctga agctatagta gtagtaccat gttgtcataa
     5641 ggagttatta gggcaatata gatatgaagc tatggaacca atattaaaac atggtgtatt
     5701 taaagctaga tttgcagatt taataactga tggtttaaga acattgttat tggagggcaa
     5761 tggatatgat acttctgtag tagaatacat atctccactt gatacaccaa aaaatttaat
     5821 gataagagcg ataaaaacta aaacgaataa tgacaaagca ttaaaagaat ataaagaact
     5881 gaaatcacaa tttggagtgg aaccaacctt agaaaaactt atatattagt taaaaatgta
     5941 aaatttacga aaagcttgct taaatatgca agctttttgg tattatatag gtatagtcat
     6001 atccagagtt ttgatttata attaaagctg tgctataata tttgattaat caaatcaata
     6061 aatgttacat ggaggtaaaa atgaacaaat tgaatatatg tattgatata gatggaacaa
     6121 taacaagccc ttatcatttt cttccatacc taaatgatat ctttaataaa aatataactg
     6181 aagcagagtg tataacacat aattgggaag aattgtatgg ttcgggaata agagatgtgt
     6241 atgcggagtt taataataag tatatacact catatgatga agcaaagatt gtagagggtg
     6301 ctacagaggt aattagcaca ttatctaaag gacataattt atcttttgta acggctagac
     6361 atgagtgttt aactgatgta actaaaaatt ggctatcgag acaaggattt tcagatatag
     6421 atgtttactt attaggaagt gattataaag ttgaaaaggc aagagaactg agttgtgaca
     6481 tatttataga agataatcct ttaaactcag tgcaactagc tgatgatggt gttaaagtaa
     6541 tacttctgga tactaactat aataaagatg ttaaacatca aaatatagtt agagttagta
     6601 attggataga tattgaaaag attataaaac aaggtattta aagatttgta gattgaaagg
     6661 gggagcatat gatagattta aaaattgaaa atagaaaagg aaaaatccat gtaaagagca
     6721 gtgaaataaa agacatttta gaattacgtc cagacttcga atatgtacaa gatatatcaa
     6781 atactataaa tcaagaaaat atattggtat ttgattgcca gttgacagga aattcttttg
     6841 atatggatga tttagatata gaagaaatat tggaagagtt aggtgaagaa atagatgagt
     6901 cttatttcag tgttttattt gaagatgtaa gagcgtattt aaaagatgct actgatgaga
     6961 tagaagcaga cttacaggat aattacttat ttgataattt gagatgttat tttgatatat
     7021 acaatataaa tcaagaattt acagatttta agtttgtatt tttagtttct tttaaagata
     7081 taaaaatttc ttcattagca aacctagcta agattgtttc taagagacag cttataggtg
     7141 catctaagtt ttatagttaa aatgaaatat tattataata aaagctagta accttgtaaa
     7201 aatataggtt actagttttt tatttattca ttttcacaaa atttcagaaa actatttaca
     7261 ttctaaaaaa agtaatgtat attaaacata atgtacatta taatggataa aaagtttcta
     7321 ttaaattact aagtttattt aataatcaaa ataatttatc ttatattagt atgtattagg
     7381 gggtatttat gcaaagttta aatgaaataa ttgctgaaaa tttaaaaaag ataaggaaag
     7441 aaaaacatct tagtttagac aagattgcac aacttagtgg tgtaagtaag agtatgctat
     7501 ctcaaattga aaagtgtgag gtaaatccta ctatatctac tttgaagaaa ataactaatg
     7561 ggctaaaaat ttcatttaca tcattgatgg agaggcagga aagtgacata gaattgattc
     7621 aaaaaagtga tatagaccat tttattgaag ataatggaaa gtatataagt tatccaatat
     7681 ttccatttga ctccaaaaga agatttgaga tatttatgat tgaaatagaa gaaggtggaa
     7741 atttagattc aaatgctgaa cttccaggaa cacaagagtt tataactgtt tttagtggcg
     7801 aagtaaccat taaaataaat ggagaagatt acattgtatc tagtggaaat tctataagat
     7861 ttaaagctga tgtatcacat atatatagaa actcaggcaa aggtatagct aaaatgagta
     7921 tggtagtata ttatatataa ataatttaat ggaattagag tgatatttag atgattttag
     7981 gtatgaagat atattatatt taagttatag gagtgtgatt tatggatgaa taataaaaaa
     8041 gcagtttttt tcttttgcag tgatttaaag aaagacccag tagccagtaa tgtgataaaa
     8101 tgttgtgaag aaataatgga tttaaatcaa actgatattg ttattgatga taattttgta
     8161 ttagagttta aggataaaaa caataactta tttcattttg taaaaacaaa agatgtaata
     8221 agtcataatt ataaatatta tcttccaatt ttaaatagac attttagaga ttatgatttt
     8281 gcaggagttg taaattggca tgagggagaa aatgctccag aacatatcct aacagctcat
     8341 acaattggag atgttccaac aggagaattt ggaaattcaa atccaagata ctttaaaaat
     8401 ctaatcaatg ctattgagga tataaaaaat gaaaatctac ttgatgaatt tactacatta
     8461 acagaagcaa ctcactggtc tggaacaaca tatggtgaag aatctaaatt aattacagaa
     8521 tattctgtac ctatgttgga tattgagata ggaagtagta gtgatagttt taataatagt
     8581 atagcaatac aggtattggc aaaatcatta attagagtat ttgattgtga tgaacctttg
     8641 aagactttgt tatgtgttgg aggggtacat tttgagaaat ctttctcaga tattataaaa
     8701 aataaagaat ataacatatc aattggacat gtattgccaa atcaatggat tgtaagtgga
     8761 atgtatgatg atgaatctgg atttgagaaa ctagaaaagt gtataaatag cattgaagga
     8821 ggaattgatt gtatagtttt tcatgataaa ttaaaaggta cctataaaga gcaatgccgt
     8881 aaactaggtg aaaaacttaa tgtacctgta tttaaacata aaatattaaa aaatccaaaa
     8941 gacttaccta tatggtagtc ttatacagtt aatataacaa taagtcggat taatttttaa
     9001 gcgaggtcaa aaatgatatt atcttatttt gcattattaa ttagagtagt gctattagct
     9061 tttgaaagag tagttgtaag attattaggt gatgaagaag gagatattta taaaaatata
     9121 gcttcatcat ttctattttt ctttataggg ggagtatgtt tacttccatt tagcataatg
     9181 gatagagtta gtgattggtc gtttttaata ccatgttata taagtagttt agtctattca
     9241 attggttcag tagcgtatgt aacgtcttta gcaactgggg aagtttcact tgttactcca
     9301 ataaatagtt taaattcatt gtttttactg cttttatcag ttatattttt aggtgaaggt
     9361 ttaagtttag caaaagttgc aggaatcata attatgattg gtggagtgtt tatattaaaa
     9421 aaagtaagtt cttttttaaa gaatgtctct gtaattttta acaatttacc atgtagatta
     9481 atgtttttat acatattttt gcagtcagta ggaagagtat tggataaggc tttttttgta
     9541 caagtatctc ctatattata ttcaactata ctgtattttt tcgtaggttt aaatttattt
     9601 atctttttag catttaaaaa gaaacaaaaa ataatttgtg agatttttac taataaaaag
     9661 gggctttcaa ttttaagtgg tgctattaat ggatattctt atttagcatt attaattgct
     9721 ttaaataatc tagaacttag tatagcagag ccattttctc aagtatctat gataataact
     9781 ttaatacttg ctcattttat attcaaagaa aatataaaag aaaaaatacc aggttcaatt
     9841 ttgatactaa ttggtgggtg gctactgtta ctttaattga cataaaaaat gaattataaa
     9901 ggtgctatta gttagtatca gaaaatttaa aaaagtattg acaattcttt taggcaaatg
     9961 ttaatataat aatacaaaat agtataaata gaagacatgt tactggtaat gcaggcagga
    10021 tcgaatatat tgctttgatt tttcaaggtg tttatatagg ttctgccttt tttgcatctt
    10081 gaatggccat tcataaaaaa ttatggagaa ggagattaca agatgaatta taaaaaaact
    10141 gccattgaca ttattgaaca agttggtggt tcaaaaaata ttttagcttt agaacactgt
    10201 tcaactagac tacgctttaa gttgattgat agaagtaaag tcaatcaaga tgctttaaaa
    10261 aaagtacaag gtgttatgag tatagttaat ggggcacaat ttcaagttgt cattggaaat
    10321 agtgttgttg atgtgtatga tgaaatttta aaagtttgcc ctttagatac aaatgatagt
    10381 actgtaaaaa ctgaagaaaa aataaactta ggaacaagat ttatagaatt tattattaat
    10441 atttttcaac cactgatttt tgctattgca ggagctggta ttttaaaatc actgttgatg
    10501 ttattagcaa tgtttaatat tttaaaatca gattctacta tatatacatt attagttgct
    10561 atttcagatg caacattcta tttcctacct cttatggtag ctgtaactac tgccaatgta
    10621 ttgaaatgta atcgtttagt agctattgcc gctgtaggat acttattgct tccagcaaca
    10681 acaactgctt taagtgaagg tgttgaacta tttggattta caattccaaa cattgcctat
    10741 aatgcacagg ttttcccggc tatcttatgt gtgtctttct tggcaacaat ggagaaaatt
    10801 tttaacaagt attcaccaaa accaattcga actttctttg ttcctatgat gtctttggca
    10861 attactgttc caattactct aactatttta ggtcctttag gatataacgt tggtacaatt
    10921 tttacaacca ttattttatt cttatataac aaaatgggat tcttagttgt aggattactt
    10981 gctgcttttc ttccatttat ggttgcaaca ggaatgcaca aagcactaat accttattca
    11041 attaatacaa ttggaaagtt gggttatgaa gctttatata tgacatcttc attagcccac
    11101 aatatttctg aaagtggtgc ttgttttgca gtagccttgc gaacaaaaga tgaaacatta
    11161 aaacaaacag cattatctgc aggaatttct gccttgatgg gaattacaga gccagcttta
    11221 tatggtatta ccttacaaca taaaagagca atgttaggtg ttgtattctc aagtgcaatt
    11281 tctggtacct ttttaggatt atttgcagta aaaggatttg taattgttgg accagggctt
    11341 gccagtatga gtatgtttat tgacccaaac aatggaaaca atttgatttt tgcgagtatt
    11401 ggttttgtta ttgcaatatt aggctcgttt attattacac tgattatttg gaaggaagat
    11461 acaaatacag tagagtcatc aattggagat gttgttgaag aagagctaga agaagaaaaa
    11521 gaagtaaata tcttagtaac tccagtagaa ggaaaagtaa tagatttaag taaggttaat
    11581 gatgaactat ttgcaagtaa gactttggga gatggagttg caattatacc aaccaatgga
    11641 aatttatacg caccttgtga ttcagaagtt gtgatgttat ttgaaacaaa acatgcaatt
    11701 ggtttaagaa caaaaaatgg ggctgagatt ttgattcata ttggaataaa tactgtaagt
    11761 atgaatggag atggatttaa aacatttgtt aagacaggcg ataatgtaaa agaaggagat
    11821 ttattaattc aatttgattt agacaaaatc tcacatgcta atttagattc aactgttatg
    11881 attgtaaaca ataatggagc agattatgct tataaagtgt taaaccagtc ttatggcaat
    11941 gtaaaaaaag gaagtatctt gtttgatgta aagggaggaa tataaatatg agaaaatttc
    12001 cagaaggatt tctttgggga ggagctacag ctgctaatca atttgaaggt ggttggaatt
    12061 taggtgggaa agggtggtct gtatcagatg tagcaaaggc acactttgat gcagatgtga
    12121 aagattataa atctaataat gaaatcacaa caaaagatat agaagaagga ttagcacatc
    12181 ctgaagatga agtaaattat ccaaaacgtc atggtagtga tttttatcat cactataaag
    12241 aagatattgc tctaatggca gaaatgggat ttaagacata tcgaatgagt attgcttggt
    12301 ctcgtatttt tccaaatgga gatgataaag agccaaatga agaaggttta cagttttatg
    12361 atgatgtatt tgatgagttg ataagttatg gaattgaacc acttgttact atgtcacatt
    12421 atgaaccacc attaaatatc gttttaaact atgatggatg gtattcacga caagttatca
    12481 atatgtttgt taggtatgta gaaactatct gtgaaagata taaaaacaaa gtgaaatatt
    12541 ggcttacatt taatgaagtt gattctatga ttcgtcatcc gtacactact ggtggtttag
    12601 ttcgagaccg ttttaaggat aaaaactttg aggaagttat ttttcaagct atgcatcatc
    12661 aatttgtagc tagtgccatt gcaacaaaaa tttgccatga gattattcca aattcaaaag
    12721 tagggtgtat gttaacaaaa ttaacgtatt atccatatac atgtagacca gaagatgttt
    12781 tggcaactca acaaaaaatg agaagtattt atgcatatag tgatacgcag gtttttggtg
    12841 agtatccagt atatttactg tcgtatttta aaaataataa cattcaaata gtaaaagaag
    12901 aacatgatga tgaaattatg aaaaagtatc cagtagattt tatttcattc tcttactata
    12961 tgtcttcttg tgaggcagct gatacaactg gattagatat tacacctggg aatacgttat
    13021 tagctgtaaa aaatccttat ttagaaatga gtgaatgggg ttggcaaatt gattcaatag
    13081 gtctacgtat atctttgatt gaattatatg atagatacag aaagccttta tttatagttg
    13141 aaaatggatt gggagctaaa gatattttaa ctgaagataa aaaagttcat gaccaatata
    13201 gaattaatta tttaaaagaa cactttaaat gtatgttaga tgcgattatc gaagatggcg
    13261 ttgaactttg gggatatact tcatggggtt gtattgattt ggtcagtgaa tcaacgaaac
    13321 aaatgtcaaa acgttatgga tatatttatg tggatgctga tgactatgga aaggggacat
    13381 ataatcgata taagaaagat agtttttatt ggtataaaaa ggtaattgaa aataatagta
    13441 tagattttaa atgaccaata gaaggtgagc aaataaaatg aactacataa taaagaaagt
    13501 tttgaactcg agcgttgttt tagttcatga tgcaaagtat aatgaattta ttttgctagg
    13561 gaaaggaatt ggatatggta aaaaaacagg agaatatatt tgtggttcca atgataatca
    13621 aatgtttgtc cctgttgaaa atacaaaatc aaaacaattt ttagaattaa tggatgatat
    13681 tcctgtcgac attttaaaga taacacagga aataatagta gaggcagaaa agttattgaa
    13741 ctcatctttt aataaaaact tatacttaat attagcagac cattttaact ttgcaattga
    13801 aaggatgcgt aaaggaataa aaattacaaa tcgagtattt tgggaaataa aaaattatta
    13861 tccaaatgag tttaaggttg gaatggttgc aatttatatg gtagaggaga gattgggcat
    13921 tcaattacca gaggaagaag cagctaacat tgctttccat tttgcaaatg ctatggcttc
    13981 agatggaaat tcatatgata cgattaagta tgccaaagta attggagaaa taattaatat
    14041 ttttgttttt tctttgaata gaaccttaga taaaaagagt atgcattata tgagatttat
    14101 aacacatata aaattttttg tggaaagatt tttttcagat agtatgttaa gcagtggtga
    14161 tgatttatta tttaatcaaa tgaaaaggtc aaatccaaaa gaaatgttaa ttgcattaaa
    14221 agttagagat tttttagaaa agaaatatgg aaaaaagtta acaaatgagg aaatagcttt
    14281 cttggttgta catatcgcac gagttaagca atagagtgaa ttactaatat tatcttatac
    14341 gtctacatgg tagaaaattg aaagaaggta aagtatataa agaaactgag acatattatt
    14401 ttgttgttca ttatgtttat tttatgagtg tatcctgtta aaaatttaag ttaatattta
    14461 taattaaaat atacaaatag ttaaaataag tttatgtggt tatgaattta ttttaactat
    14521 ttttttgttt aatggaaaat atgttttata actagaattt tatttttaat taattataat
    14581 acaaaaaact ttataaaatc ttca
//
LOCUS       DAYAPA010000110        14564 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.111, whole genome shotgun sequence.
ACCESSION   DAYAPA010000110 DAYAPA010000000
VERSION     DAYAPA010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14564
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.111"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            210..1838
                     /gene="aspD"
                     /locus_tag="ACGI59_002969"
     CDS             210..1838
                     /gene="aspD"
                     /locus_tag="ACGI59_002969"
                     /EC_number="4.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047688 - aspartate 4-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006531 - aspartate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 4-decarboxylase"
                     /protein_id="HFG9740748.1"
                     /translation="MKNHTYKEIKNIYGKISPFEFKDKLIDIAKYTAKENNRELLDAG
                     RGNPNWTCSTAREAFFTFGHFAITETRSNWDLGHLAGMPQKKGIKERFFKFINENIDM
                     PGAYLARDIINFGINELGFDGDEFVHELADGIIGDNYPLPDRMLPHMEKIVHDYLVQE
                     MKYDISGKYGDVEIFAVEGATAAMCYIFDSLMANELLKKGDTIALMTPIFTPYLEIPN
                     LPRYDFKVVNINANEVDEKGAHTWQYTKKELEKLRDKSIKALFVVNPNNPASIAMDET
                     SCNNLIDVIENYNKDLMIISDDVYGTFVEDFSSLMSKLPYNTVGVYSYSKYFGVTGWR
                     LGTFALHKKNVFDKKINDLTGELKKSVDKRYSDMSLNPSSLSFMERVVADSRLVALNH
                     TAGLSTPQQVQMAFFSAFALIDKVDAYKKLNMSICHRRQKLLFEALELPINENKNNAA
                     YYTQFDIEEWAKLNYGEDLFKFISKTASPVDVLYKLANDYSVVLLSGNGFYGPEWSIR
                     ISLANLYDEAYTKIGKAIREILNGYIVEWQKIKK"
     gene            1959..2954
                     /locus_tag="ACGI59_002970"
     CDS             1959..2954
                     /locus_tag="ACGI59_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFG9740749.1"
                     /translation="MSIKKKVAIVFTGGTISMTVDDKVGAAIPTLSGEQILSMVTNID
                     KVADVEVFNFDEIPGPHITPYRMMELKNYVNDLLARKDITGVVITHGTDSLEETAYFL
                     DLTIDNIKPVIVTGAMRSSSELGYDGSSNLSASVCTAVSKDAMGKGVLVVLNNEVLLA
                     SEATKTNTLSLNTFKSLTSGPLGIIDCNELVLTRDIVNRTIIDTDKVESKVALFKAYV
                     GENADFIRFAVDSGYKGIVIEAMGRGNIPPQMLSGVEYAREKGLPVVIVSRCHSGRVF
                     DSYGYFGSGRDLKNLGCIFGGDLPGQKARIKLILALGKTDNLDEIKDFFEKGIYC"
     gene            complement(3132..3440)
                     /locus_tag="ACGI59_002971"
     CDS             complement(3132..3440)
                     /locus_tag="ACGI59_002971"
                     /inference="COORDINATES: protein motif:HMM:NF015898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HFG9740750.1"
                     /translation="MSKDVADEIEKTYAWKHTTTITLLARLTKKGFFISQRIGKHVHY
                     RVLIKEKEYLKIEGKRIFGGLHNNPLSELISKLHDGEEITEEKIIEIGNWIKSWKDED
                     "
     regulatory      3742..3839
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     repeat_region   3980..4472
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=3980..4009
                     /rpt_unit_seq="aatttacattccatatagttaatataaaac"
     gene            complement(4758..4916)
                     /locus_tag="ACGI59_002972"
     CDS             complement(4758..4916)
                     /locus_tag="ACGI59_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9740751.1"
                     /translation="MDNFLQGILASLSASLIVYIASKLFRKRKKPLKAATKSGWEFDL
                     KIRFHKTK"
     gene            complement(5210..6580)
                     /locus_tag="ACGI59_002973"
     CDS             complement(5210..6580)
                     /locus_tag="ACGI59_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp29"
                     /protein_id="HFG9740752.1"
                     /translation="MKLKKLLSLGLVSMFVAGFPISANALDKIDKIQGADKYETAGII
                     ADKQSYTTAILINADSTMADGLSASGLAGATNAPILLTKKNNIPNATLKRVEKAKKVY
                     IIGGESSIDKATETFLKDKGIETRRLQGNDRIKTSYNVAKEINSINKVNKVILTNAFK
                     GEPDAMSAAPVAVRDKAAIVLTDGKSVPFNTTGIESYAIGGTSSMSDSLVNDTNSTRL
                     GGVDRYDTNKKIVNKFYNGAKEFYIASGTDLVYALVASPMAKNNPIVLVDIGSNKDIL
                     KNAAKVTGIGNLSDKIMLECENTINNISLPSSNKNIICGLKIDFDSNHIIWQDKGSYL
                     ECYDDNLEYYTEKYKDPTVPINIENTSTQNLDVTKLKVYGEANSTLYMGQYAKIKMDF
                     NSFGVLKPGEKCTIYLDIISLDPSRSMTLTFSYVGDKENYKYEQGLASRILFYNPDGE
                     DFFEYK"
     gene            6796..7611
                     /locus_tag="ACGI59_002974"
     CDS             6796..7611
                     /locus_tag="ACGI59_002974"
                     /inference="COORDINATES: protein motif:HMM:NF026010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
                     /protein_id="HFG9740753.1"
                     /translation="MNIKSAFIRKRGEKFYVYVEYIEETTGKIKQKSYGSYEKKKDAE
                     KHLIEIKSTINNNKFVAPKDMTFVERCYKYMDMNKNNWSPYTVTNTKSWVKNYIEPLF
                     KNLKLTEVNPSSVQAFVNYNFNNSTFTSAKVRYSFLSSVIREAYRLREIQENSCDFIK
                     LPSKEKYSTNEIYNRKEVLLLIEKLLSNNIEIPILLMLLLGLRMGEATGLRWSDVDLD
                     NNLINVNQILVYSNGKISFKEPKTPKSKRALSAPKELIEKLKIKKLRQSKLKL"
     gene            7641..7913
                     /locus_tag="ACGI59_002975"
     CDS             7641..7913
                     /locus_tag="ACGI59_002975"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFG9740754.1"
                     /translation="MSKHQFKSVSTFFTKKNVSQFYKKNNLKKIRIHDLRYTSATLLL
                     LGGTNMKVVSERLGHTDIKITMNRYSHVLKEMDKEASDNLSELLFK"
     gene            complement(8276..8356)
                     /locus_tag="ACGI59_002976"
                     /pseudo
     CDS             complement(8276..8356)
                     /locus_tag="ACGI59_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005541856.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(8396..9064)
                     /locus_tag="ACGI59_002977"
     CDS             complement(8396..9064)
                     /locus_tag="ACGI59_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFG9740755.1"
                     /translation="METVTNNQSGVEIQLSLFEAIKDLLLINPIFLLGIILIGIFLVV
                     IFKKNSDIPKVKTSILSLAMYYYLCLTLSHIVGIPTFSEYIRLSQLGETFFNPNINLI
                     PFSDGFSLSFILNILLFVPLGFLCPLISKTFERLRNTFLIGFGLSLFIETIQLFTLHR
                     ATDIDDLLTNVIGTIIGYFCFKLIAKLRIVKLHSDDDSMEQDYTVCIPIAIIIVALIL
                     GFFS"
     gene            complement(9917..12337)
                     /gene="leuS"
                     /locus_tag="ACGI59_002978"
     CDS             complement(9917..12337)
                     /gene="leuS"
                     /locus_tag="ACGI59_002978"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFG9740756.1"
                     /translation="MSVYNFKEVESKWQKIWKDNNQYKMDTAQTEKPNYYTLEMFPYP
                     SGKIHMGHVRNYSIGDVVARFKKMEGYNVLHPMGWDSFGLPAENAAIKHGIHPHKWTM
                     ENIEEMKEQLNLLGISYDWDKEVATSTPEYYRFTQEIFLKFLEHGLAYKKKSYVNWCP
                     SCETVLANEQVVQGACERCKATVLKKDLEQWYFKTTEFAEELLNDLDILDGWPEKVKT
                     MQKNWIGKSTGADLVFDIDGTDKSMTVFTTRPDTTYGVTYMVLAPEHELVKELVAGTE
                     YEADVEAFVQKMHTMTEIERTAADVEKEGMFIGRYVINPLNGKKVPLWIANYVLVEYG
                     TGAIMAVPAHDERDRDFAEKYNLDIIDVITEDNKMINSEEFNGLDASEGFEGIIDKLE
                     KEGRGKRTINYRLRDWLVSRQRYWGCPIPVVYCDECGIVPVKKEDLPVLLPTDVEFTG
                     KGESPLTTSKQFMSTTCPHCGKPARREVDTMDTFVDSSWYFLRYVDSNNENEPFSKEL
                     VNRWHPVDQYIGGVEHAIMHLLYARWFVKAFKSMGMVDFNEPFKNLLTQGMVLMDGSK
                     MSKSKGNTVSPMDIIDEYGADTARLFVLFAAPPERDLDWSEQGVDGCFRFLNRVYRLV
                     DELADVVKKDVEFGELNSQDKDMRYTIHSTLKKVTADLSEKFGFNTAISALMELINDM
                     YKYKELDNINEAVIKEGVQTIVTIIAPFAPHLGEELWTMIGKEGSVFDIDWPKYDEKA
                     LVKDEIEVVVQVNGKVRGKLTVNSNISKDEMEKVALEDEKIKGLVEGKTIVKVVAVPK
                     KLVNIVVK"
     gene            complement(12691..13041)
                     /gene="rsfS"
                     /locus_tag="ACGI59_002979"
     CDS             complement(12691..13041)
                     /gene="rsfS"
                     /locus_tag="ACGI59_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFG9740757.1"
                     /translation="MTVEQMTKIAYDAIEDKLGQDTVIINIGKVSSLCDYFIITTASS
                     QRQVKAIADNVEDELAKLGLEPRGKEGQGTQTWVLLDYGDIMVHVFNEENRGFYNLEK
                     LWKDAPYIDIDTLA"
     gene            complement(13158..13730)
                     /gene="yqeK"
                     /locus_tag="ACGI59_002980"
     CDS             complement(13158..13730)
                     /gene="yqeK"
                     /locus_tag="ACGI59_002980"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HFG9740758.1"
                     /translation="MNLENINQRLNQMLPVGRLSHSKNVAKCAEKLCEIYGCDKEKAY
                     LAGMIHDCAKYLSDKEIEDYVNKYEIYLDPIEDGNRSLSHSVIGAYICEYEFEVEDED
                     IINAIKYHTTGREDMSLLEKIIYIADLIEEGRKFPVVDTLRELAYGGKLDEALLTSFN
                     NTLMFVINKKEEIHPRTVMARNYLIKEKLL"
     gene            complement(13731..14420)
                     /gene="nadD"
                     /locus_tag="ACGI59_002981"
     CDS             complement(13731..14420)
                     /gene="nadD"
                     /locus_tag="ACGI59_002981"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFG9740759.1"
                     /translation="MRSENLVKMAELKNTEKLEKFNRHKGKIKIGILGGTFDPIHYAH
                     LATAEFIRDKYDIDKIIFIPSGNPPHKLCITTDKYDRYNMTLLATESNEDFLVSKVEI
                     ERKKRTYTIDTLKYLKKKYKNADIYFITGADAICSVEEWKDVKKNFELATFIAATRPG
                     ISLLRSQETIEKLTKKYNADIITVYVPSLDISSTYIREQLNEGKSIRYLVPENVENYL
                     YENKLYQYGDD"
BASE COUNT         4912 a         2371 c         1841 g         5440 t
ORIGIN      
        1 aattgaatat ttattttata taacaatcat tttttattaa aaggtatttt ttattttaac
       61 atatatttaa taaggctaaa tagagttatt attttaattt catactttac tttttctata
      121 aacctattat aattaaagta taatcatttt tgcaacttgt ttattttatt ataaaaatgt
      181 gaaacttatt acataaggag gaatttaaca tgaaaaatca tacttataaa gaaatcaaaa
      241 acatatatgg aaaaataagc ccttttgaat ttaaggacaa attaatagat attgcaaaat
      301 acactgcaaa agaaaataac agagaattac ttgatgcagg tcgtggaaat ccaaactgga
      361 cttgttcaac tgctagagaa gcctttttta cttttggtca tttcgctatt actgaaactc
      421 gttctaattg ggatttaggt catttggctg gaatgcctca aaaaaagggt attaaagaaa
      481 gattttttaa atttataaat gaaaatatag atatgcctgg agcatattta gctagagata
      541 ttataaactt tggtataaat gaacttggat ttgatggaga tgaatttgtt catgaactag
      601 ctgatggaat tattggtgat aactatccac ttccagatag aatgttacct catatggaaa
      661 aaatagtaca cgattaccta gttcaagaaa tgaaatatga tatatcaggt aaatatggtg
      721 atgtagaaat ttttgctgtt gaaggtgcta cagctgctat gtgctatata tttgattcat
      781 taatggcaaa tgaacttcta aaaaaaggag ataccatagc cttaatgact ccaatattta
      841 caccttatct tgaaatacct aaccttcctc gttacgactt taaagtggta aatataaatg
      901 ctaatgaagt tgatgaaaaa ggtgctcata catggcaata tacaaaaaaa gaattagaga
      961 aacttcgtga caaatctatt aaagctttat tcgttgttaa tcctaataat cctgcttcta
     1021 tagctatgga tgaaacatct tgtaataatc taatagatgt aattgaaaat tataataaag
     1081 atttaatgat aatatcagat gatgtatatg gaacttttgt agaagatttt tcatccttaa
     1141 tgtcaaaact accttacaac actgttggag tatattctta ttcaaaatat tttggagtta
     1201 caggatggag acttggtaca tttgcccttc ataagaaaaa tgtttttgat aagaaaataa
     1261 atgatttaac tggagaatta aaaaaatcag tagataagcg ttacagtgat atgagtctta
     1321 acccttctag cctttcattt atggaaagag ttgttgctga tagcagactt gttgctctca
     1381 accatactgc tggtctttct actccacagc aagtacaaat ggcgtttttc tctgcttttg
     1441 cattgattga caaagtagat gcctacaaaa aactcaatat gagtatctgc catagacgtc
     1501 aaaaactatt atttgaagct ttagagcttc caattaatga aaacaaaaac aatgctgcat
     1561 attacactca atttgatatt gaagaatggg ctaaattaaa ctacggagaa gacttattta
     1621 aatttataag taaaactgca tctccagtag atgttcttta taaattagct aatgactatt
     1681 cagtagtttt acttagtgga aatggattct atggtcctga atggtcaatt agaatatctc
     1741 tagcaaatct atatgatgaa gcttatacta agataggaaa agccataaga gagatattaa
     1801 atggttatat cgttgaatgg caaaaaatta agaaataatt atattacact gaaatatcta
     1861 aattatatga ataaaagagt ttatgtagac cttaactact ttttttgata taatcctatc
     1921 ggaattataa attttattta tgaaaggcag agaatagtat gagtattaag aaaaaagttg
     1981 ccatagtttt tactggtgga actatatcta tgacagtaga tgataaagtc ggagcagcta
     2041 taccaacttt atcaggtgaa caaatactat caatggttac caacattgat aaagttgcag
     2101 atgttgaagt ttttaatttt gatgaaattc caggacctca cataacacct tatagaatga
     2161 tggaattgaa aaattatgta aatgatttat tagcaagaaa agatattaca ggtgttgtaa
     2221 taacacatgg tacagatagt ttagaggaaa cagcttattt tctagacctt acaattgaca
     2281 acatcaaacc tgtaatagtc acaggtgcta tgagaagtag ttctgaactt ggatatgatg
     2341 gttcaagtaa tttatctgca tcagtttgta ctgcagtatc caaagatgct atgggaaaag
     2401 gagtccttgt agttttaaac aatgaagtat tattagcttc tgaagctact aaaacaaata
     2461 ctctttctct aaatacattt aaatctctaa ctagtggccc attaggaata attgactgca
     2521 acgaacttgt ccttacaaga gatattgtaa atagaacaat tatagatact gataaggtag
     2581 aatctaaagt tgccttattt aaagcatatg taggagaaaa tgctgacttt ataagatttg
     2641 ctgttgatag tggttataaa ggtatagtta ttgaggcaat gggtagaggt aatattcctc
     2701 ctcaaatgct ttctggagtt gaatatgcta gagaaaaagg acttcctgta gttatagttt
     2761 ctagatgtca ttcaggtaga gtattcgata gttatggtta ctttggttct ggtagagatt
     2821 taaaaaatct tggttgtata tttggtgggg acttaccagg tcaaaaagct agaataaaac
     2881 ttatcttagc acttggaaaa actgataacc ttgatgaaat caaagatttc tttgaaaaag
     2941 gaatctattg ttaattctaa taacttctat ttaattattt attgatattt caacattttt
     3001 aaatcaaaat ctaatactta tgtcagttaa atgtcagcaa aatattttat ccacaagttt
     3061 tccacaactt atccccaata ttatccacaa gaaaaaaggt agcacttata ttagtagcta
     3121 ccttttattt attaatcttc atctttccag ctttttatcc aatttcctat ttctattatt
     3181 ttttcttcag ttatttcttc tccatcatgc aattttgaaa ttaattctga taatggattg
     3241 ttatgtagac ctccaaaaat tctttttccc tctattttga gatattcttt ctcttttatt
     3301 aatactctat agtgtacatg tttcccaatt ctctgcgata tgaaaaaacc ttttttagtt
     3361 aatctagcta aaagtgtaat tgtagttgta tgcttccaag catatgtttt ttctatttca
     3421 tctgcaacat cttttgatat tacagtttta taaccagtat tctatatgta tttcataatt
     3481 tttaattctg catctggtaa ttttttattc aacataatat attccttctt tcaatttatg
     3541 tcacttcctc aaacttatct agcttaaatt aataatagga gctttttatc acaaaaacta
     3601 caggtaaatt tttctatata ttctacgtat ttagtctgtt ttttaagtgt gaaattacgt
     3661 tcatacctat aaatttattc aattttcaaa caatcttatt gaataataac taattctatt
     3721 gtattattta gttatagaat aaaactaaat cggcaaaact agagaaatct agtgacgcaa
     3781 agctatagag actaagactt atataaatat cttatgagtt atgtcagcca gttgccaaag
     3841 agatattgtt ctttttgttt ttatgaaagt tttttagggg gataataatt tatatattta
     3901 gagataaaat agataaatgt acacatatat tgactgctta tattagcagt tcgtatgatt
     3961 attgtaattt tttggataca atttacattc catatagtta atataaaacg ttatatactg
     4021 aaagcggtga aactggttac gtagcaattt acattccata tagttaatat aaaacctgtg
     4081 cgtgtaggag atggtaacac aactgttgaa gaatttacat tccatatagt taatataaaa
     4141 cacacatcta catgtttttc tatctgagtt catctctaat ttacattcca tatagttaat
     4201 ataaaacttt ttaatccttc ttctgaactc ccaaagttta tatcatttac attccatata
     4261 gttaatataa aacccacgaa atttaaggtc gatatatcag atttgaaaaa atttacattc
     4321 catatagtta atataaaaca agctaaatgg agcaaccgac caagtcgcta gaaataattt
     4381 acattccata tagttaatat aaaacttttc tattaagtgt aagcagaatg catggaatat
     4441 gaaatttaca ttccatatag ttaatataaa accccaaaat aaattgagta tttccaatgc
     4501 ctacacatat acaaatctct caaatttgca gtgaaccatg agtagtgcaa ttgataacac
     4561 ttatcacaag tcctcaatgc cttatatttc aactgttaag ccttaatttc ttcaaaaaat
     4621 cgaacactgc aaaatttcta tgactttatt atatcataaa aatattattt ttgaatatct
     4681 gtaccaattt gtgatataat aaaagcaaga agaactacaa tctattttgc tgtagagtgg
     4741 agttcataat ttagaaatta cttagtttta tggaatctga tttttaaatc aaattcccaa
     4801 ccactcttag ttgccgcttt gagtggtttt ttacgctttc taaatagttt gctagctata
     4861 taaactatta agctagcaga tagacttgct agtatgcctt gtaaaaaatt atccatgtat
     4921 attcacctcc cttcttaacg tcgggaggat aattttttga tacatgaact ccactctata
     4981 aattgtagat tacatcttct tgctaaaaat attataacat ataattctta catattttac
     5041 ctatttatta ctttttacat tataataata ttcttttaat caaaaaaaca cctactatta
     5101 aagtaaatgt ttttaagtat ttttaatttt aaaatccata tagttcatat gaaactaaat
     5161 ataataactc aacatatgag caatatcgag acatttacat acccactagt tatttatatt
     5221 caaaaaaatc ttctccatcc gggttataaa ataatattct acttgccaat ccttgttcat
     5281 atttataatt ttctttatca cctacataag aaaatgttaa tgtcatgctt cttgatggat
     5341 ctaatgaaat tatatctaaa taaatagtac atttttctcc tggctttaat acaccgaaac
     5401 tattaaaatc catttttatt ttagcgtatt gccccatata taatgttgaa ttagcttctc
     5461 cataaacttt taattttgtt acatctaaat tttgtgtact tgtattttct atatttatag
     5521 gaactgttgg gtctttatat ttttcagtat aatattcaag gttatcatca taacattcaa
     5581 gataacttcc tttatcttgc caaattatgt gatttgaatc aaaatcaatc tttaatccac
     5641 atattatatt tttatttgaa gatggaagac taatattatt gatggtattt tcacactcta
     5701 gcataatttt atcacttaga tttcctatcc cggttacttt tgcagcattt ttaagtatat
     5761 ctttattact tccaatatca actagtacaa taggattatt tttagccatt ggtgaagcaa
     5821 ctaaagcata aactaaatca gtaccactag ctatataaaa ttcttttgcg ccattataga
     5881 acttatttac tattttttta ttagtatcat atctatctac tccacccaat cgagttgaat
     5941 tagtatcatt tactaaacta tcactcattg atgaagtgcc acctattgca taactttcta
     6001 taccagttgt attaaatgga acactttttc catcagttaa tactatagct gctttatctc
     6061 taactgctac aggtgcagca ctcatagcat ctggctcacc tttgaaagcg ttagttaata
     6121 ttactttatt tactttatta atggaattta tttcttttgc tacattataa cttgtcttaa
     6181 tcctatcatt accttgaagc cttctagtct ctattccttt atcttttaga aaagtttctg
     6241 ttgctttatc aatggaactt tctcctccta ttatgtatac ctttttagct ttttctactc
     6301 ttttaagagt tgcattaggt atattattct ttttagtcaa aaggattggt gcatttgttg
     6361 ctcctgcaag tccactcgca cttagcccat cagccattgt actatctgca tttataagta
     6421 ttgcagtagt ataactttgt ttgtcagcta tgataccagc agtttcgtat ttatctgctc
     6481 cttgaatttt atcaatttta tcaagtgcat tagctgatat aggaaaacca gcaacaaaca
     6541 ttgacactaa ccctagtgat aataatttct ttaatttcac aatatctccc ccttttatat
     6601 atgaaatact atatatctta ataattctac attaaatttt cttttcctcc tttaacgata
     6661 tttatcttcc tgttaattta agtgtataca aatacgtgtt ataatgtaac tgtaaagaat
     6721 taaaaagata atgacataat tagagtagta caactgttct tttataaaac taacttataa
     6781 agggagcgta catatatgaa tatcaaatca gcttttataa gaaaaagagg ggaaaaattc
     6841 tatgtatatg tggagtacat agaggaaaca actggcaaga taaaacagaa aagttatgga
     6901 agttatgaaa agaaaaagga tgctgaaaaa catttaattg aaataaaatc tactataaac
     6961 aataataagt ttgttgctcc aaaagatatg acatttgtag aaagatgtta taaatacatg
     7021 gacatgaata aaaataattg gtctccatat actgttacaa atacaaaatc ttgggttaaa
     7081 aattacatag aacctctttt taagaatcta aaactcacag aagtaaatcc tagttcagta
     7141 caagcttttg taaattataa ctttaataat tctaccttta ctagtgctaa agtaaggtat
     7201 agctttctaa gttctgtaat aagggaagca tatagattaa gagaaataca agaaaattca
     7261 tgtgatttta taaaacttcc ttctaaagaa aaatattcta caaatgaaat atacaataga
     7321 aaagaagtat tattgctaat agaaaagtta cttagtaata atatagaaat acctattctt
     7381 ttaatgttac ttctaggttt aagaatggga gaagctactg ggcttaggtg gtcagatgtt
     7441 gacttagata acaatctgat aaatgttaat caaattcttg tctattctaa tggcaaaata
     7501 tcttttaaag agccaaaaac accaaaatca aaaagagctt tatcagctcc aaaagaatta
     7561 attgaaaaat tgaaaattaa aaaactaaga caaagcaaat taaaattgta aggtatcctg
     7621 gaaaacagaa ataatttagt ttgtctaaac accaatttaa atccgtttct accttcttca
     7681 ctaagaaaaa cgtttcacaa ttttacaaaa aaaataattt aaaaaagatt agaatacatg
     7741 atttaagata tacaagtgcc actctgctgc ttttaggggg aactaatatg aaagttgttt
     7801 ctgaaagatt gggtcataca gatataaaaa taactatgaa tagatattct catgtactta
     7861 aagaaatgga caaagaagct tccgacaatc taagcgaact tctatttaaa tagtaaaaac
     7921 tgactgtcag ttaaatgtca gttaaaccta aaaaaatagg ttttgtcagt tgattatcag
     7981 tcaattattt tataatagtt tcacttaaaa acgtctatat ctacacattt tactatttgt
     8041 ttatgcttat taatatctat ctgttattat taattctaat agcttctatt taattattta
     8101 gttcagttta caggaaacta cacacaaacc ataaaatagg atgttgacaa tataacagat
     8161 agtatgctcc tgtaaaaggt aacgtactat ctgttatgtt gtctggattt ccaaatggtt
     8221 accctttggc acacaacttt gctatctcct tggtaaacta aaagtttgta atctaaaatc
     8281 ctaatccatc tctataaaat ttaattgact tctccatact ttttacacct aaacatataa
     8341 aagtaatttt attcacttaa tgacctccat aattcccaaa ttagctattt ggaacttaac
     8401 taaaaaatcc taatataagg gcaactatta taatggcaat cggaatacat actgtgtaat
     8461 cctgttccat agagtcatca tctgaatgta gttttacaat tcgcaattta gcaatcagtt
     8521 tgaagcagaa atagcctatt attgttccaa ttacattcgt gagtaagtcg tctatatcgg
     8581 ttgctctgtg taacgtaaaa agctgaatag tttcgataaa aagagataat ccaaaaccaa
     8641 tcaagaatgt atttctcaaa cgttcaaagg ttttgctgat aagcggacat aaaaaaccta
     8701 atgggacaaa aagtaaaata ttcaaaataa aactcaaact aaatccatca ctaaaaggaa
     8761 taagatttat gtttggatta aagaacgttt cccctaactg tgacagtctt atatattcac
     8821 tgaatgtagg gataccaaca atatgcgata gtgttaaaca taagtagtaa tacattgcca
     8881 gtgataaaat agatgttttt acctttggaa tatcactatt tttcttgaat attacaacta
     8941 agaaaatacc aatcaagata attcccaaca agaaaatagg attaattaac aataaatctt
     9001 tgatagcttc aaacagactg agttgaattt ctacacctga ttgattattt gttacagtct
     9061 ccatttgtac atacctcctc ataacatttc aattatttct aaaacacaaa atagaagata
     9121 aatcttaaaa tcatcctatc aaaattttta aatttgcctt tatcagttta taccaccagt
     9181 taattcctgt cttacttctg tatggtattc ttgggattct ttcatcaaat tagacaaaaa
     9241 atggttacca tatcaataaa ctggttacgc tccatatctc ccgaagcaac catcccctga
     9301 gaataaatcc gtatcaggaa tctccacatc tactgcacct atcacaagtt atttgataac
     9361 atcctcagac aatcccaact tggatatatc gacaacaccc attagaatac cactgctgac
     9421 catacttgtt ttcctgctga taattttaat caactgggaa gttgaaacgc tatctttttt
     9481 acaagttcct ttttagaaac atggtagcta ttatatgaat ctgaatttcg ttgtctgggt
     9541 gtttcatata aagcatagat acgcaccttt tttgcctgtt ttcattatat agaaaaaaat
     9601 ttcaaaatac aataattata tttttttcgt ttaagcttag agcagttcct tgatgaacaa
     9661 cactttggaa actgaatgat ttacatacca cttaatacat taactcgatt taacattaat
     9721 tataataata tttaaaacaa caactagggt atataagaat gaacttttta gttctttaat
     9781 catttttttg tataaaatca aatctatttt tataatttca acaattttat aatttcaaca
     9841 atgaaaaaag gcttatctct attttgagac agcctcgttt attttataaa tttatatttt
     9901 tgttcatatt tttgagttat ttaacaacta tatttactag ttttttaggt acagctacaa
     9961 ctttaactat agttttacct tcaactaatc cttttatttt ttcatcttct aaagctacct
    10021 tttccatctc atctttagat atatttgagt taacagttaa ttttcctcta acttttccat
    10081 taacttgtac aacaacttct atttcatctt ttactaaagc tttttcgtca tatttaggcc
    10141 agtctatgtc aaatacactt ccttctttac ctatcatagt ccatagttct tcacctaaat
    10201 gaggggcaaa tggtgcaatt atagttacta tagtttgtac accttctttt ataactgctt
    10261 cgtttatatt atctaactct ttatatttat acatatcatt tattaattcc ataagagctg
    10321 atatagcagt attaaatccg aatttttcac ttaaatcagc agtaactttt ttaagtgtag
    10381 agtgtatagt atatctcata tccttatctt gggaatttaa ttctccaaac tctacatcct
    10441 ttttaaccac atctgctaac tcgtcaacca atctataaac cctatttaag aatctaaagc
    10501 atccatcaac accttgctct gaccagtcca aatctctttc tggtggtgct gcaaataata
    10561 cgaaaagtct tgcagtatct gctccatact catctattat atccatagga gatactgtat
    10621 tccctttaga cttactcatt ttagaaccat ccataagtac cattccttga gtaagtaagt
    10681 ttttaaatgg ctcattgaag tctaccattc ccatactctt aaatgccttt acaaaccatc
    10741 ttgcataaag taaatgcatt atagcatgtt ctactccacc tatatattgg tctactggat
    10801 gccatctatt aactaattcc ttactaaatg gttcattttc attattgcta tccacatatc
    10861 ttaagaaata ccaagaagaa tcaacgaatg tatccatagt atcaacttcc cttctagctg
    10921 gttttccaca gtgaggacaa gttgttgaca taaattgctt tgaagtcgta agtggagatt
    10981 ccccttttcc agtaaattca acatctgttg gtaaaagaac tggtaaatct tcctttttaa
    11041 ctggaactat tccacattca tcacaataaa ccacaggtat aggacaaccc cagtatcttt
    11101 gtcttgaaac taaccagtct ctaagtctat aattaattgt tctctttcct ctaccttctt
    11161 tttctaactt atcgattatt ccttcaaatc cttctgaagc atctaatcca ttaaattctt
    11221 ctgaatttat cattttgtta tcttctgtta ttacatcaat tatgtctaaa ttatattttt
    11281 cagcaaaatc tctatctctt tcatcatgtg ctggaactgc cattatagca cctgttccat
    11341 actcaactaa tacatagtta gctatccata gaggaacttt cttgccattt aaaggattta
    11401 taacatatct acctataaac attccttctt tttctacatc agctgctgtt ctttctattt
    11461 ctgtcatagt atgcattttt tgaacaaaag cttcaacatc tgcttcatat tcagtccctg
    11521 caaccaattc tttaactaat tcatgctctg gagcaagtac catataagta actccataag
    11581 ttgtgtctgg tctagttgta aatactgtca tagatttatc agttccatct atatcaaaaa
    11641 ctaaatctgc tcctgtactt ttacctatcc aatttttttg cattgtttta actttttctg
    11701 gccatccatc taatatatct aagtcattaa gtaattcttc tgcaaattca gtagtcttaa
    11761 aataccattg ttctaaatct ttctttaata ctgttgcttt acatctctca catgctcctt
    11821 gaacaacttg ctcatttgca agaacagtct cacaagaagg acaccagtta acatatgatt
    11881 ttttcttata tgcaagccca tgctctaaaa acttcaaaaa tatttcttgt gtaaatctat
    11941 agtattctgg cgttgaagtt gcaacttctt tatcccaatc ataactaata cctaataaat
    12001 ttaattgctc tttcatttct tctatatttt ccattgtcca tttatgtgga tgtattccat
    12061 gttttatagc tgcattttca gctggtaatc caaaagaatc ccatcccatt ggatgaagta
    12121 cattatatcc ttccattttt ttgaatcttg cgacaacatc acctatagaa taatttctaa
    12181 catgtcccat gtgtattttt ccagatggat aagggaacat ttctaatgtg taataattag
    12241 gtttttcagt ttgtgcagta tccattttat actgattatt atctttccag attttttgcc
    12301 atttcgattc tacttcttta aaattatata cgctcatatt ttgcctccta tttttaattg
    12361 tgtataacta ccttatttag tattatttac ttataatttc caaataattc tttgaaaata
    12421 aaaaatcctc gtcactaaac ttacgtttag ggacgaggat aaatcttcgc ggtaccaccc
    12481 taattagcct actacatata tagactctct tagatagctt ttaatccagc catggagaat
    12541 agattttaat ctagtaagtt caaagttata taatattagt tctcaccttc cactaactct
    12601 ctgaaaaaat ataatctttt actgctctag tctattcaat ttaatataat ttttgaaatt
    12661 ttctaattat aataacatat attatcatgt ttatgctaat gtgtcaatat ctatataagg
    12721 tgcatctttc cataattttt ctaaattata aaaccctcta ttttcctcat taaatacatg
    12781 aaccattatg tctccataat caaggagtac ccaagtttga gttccttgtc cttctttacc
    12841 tcttggctct aatcctagtt ttgctaattc atcttccaca ttgtcagcaa tagcttttac
    12901 ctgtctctga gaagatgctg tagtaataat aaaataatca cacaaactag aaacttttcc
    12961 tatatttatt ataactgtat cttgacccag tttatcttct atggcatcat atgctatttt
    13021 tgtcatttgt tccactgtca tataaaaatt cctcactttc ctaattaaaa tacacttagt
    13081 tattgtaaca tattaatacg attttgtaac atagtcaaat ttaaatttat taatatgttt
    13141 gcctttatat agctttttta taataacttt tctttaatta gatagtttct tgccataact
    13201 gttcttggat gtatctcttc ttttttattg atgacaaaca ttaaagtatt attaaaagaa
    13261 gttaaaagtg cttcatcaag cttccctcca tatgctaatt ctcttaatgt atctacaact
    13321 gggaattttc ttccttcttc aattaaatca gctatgtaaa ttattttttc taaaagagac
    13381 atgtcctctc tccctgttgt atgatatttg attgcattta taatatcttc atcttccact
    13441 tcaaattcat actcgcatat gtacgcccca attacactat gagacaagga acgatttcca
    13501 tcctctattg ggtctaaata tatttcatac ttatttacat aatcctctat ttccttgtca
    13561 cttaaatact ttgcacaatc atgaatcatt cctgctaagt aagctttttc tttgtcacat
    13621 ccataaatct cacataattt ttcagcacat tttgcaacat tttttgaatg gctaagcctc
    13681 cccactggta gcatttgatt tagtctttgg ttaatatttt ctaagttcat ttaatcatct
    13741 ccatattgat atagtttatt ttcatataaa taattttcta cattttcggg tactaagtaa
    13801 cgtattgact tgccttcatt tagctgttcc cttatatatg tggacgatat atccagtgat
    13861 ggaacataaa ctgttataat gtcagcatta tacttcttag ttagtttttc tatagtctct
    13921 tgtgacctta acaaactaat tcctggtcta gttgcggcta taaaagtagc taactcaaag
    13981 tttttcttta catctttcca ctcttcaaca ctacaaattg catctgcacc tgttataaaa
    14041 taaatgtctg catttttgta ttttttcttt aagtatttta atgtatctat tgtataagtc
    14101 cttttctttc tttcgatttc tacttttgaa actaaaaaat cttcgttgct ttcagttgct
    14161 agaagtgtca tattatatct atcatactta tctgtagtga tacataattt atgtggtgga
    14221 tttcctgatg gtataaagat tattttgtca atgtcatact tgtctctaat aaattctgct
    14281 gttgctaaat gagcatagtg tatagggtca aaagtacctc ccaatatacc tattttgatt
    14341 ttgcctttat ggcgattaaa tttctctagt ttttcagtat ttttcaactc tgccatctta
    14401 actagatttt cacttctcat attttgtctc cttttaatgt tataatacta ttgtaccatt
    14461 attttttatt tcttaaagct tataatttgt ttttttgggt atataattat atttatatgc
    14521 taaaaaacac tttaaaaaat aattatttaa cataattctg cttt
//
LOCUS       DAYAPA010000111        14533 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.112, whole genome shotgun sequence.
ACCESSION   DAYAPA010000111 DAYAPA010000000
VERSION     DAYAPA010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14533
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.112"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            23..943
                     /locus_tag="ACGI59_002982"
     CDS             23..943
                     /locus_tag="ACGI59_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HFG9740760.1"
                     /translation="MYKSENFSKKSVLINSFLSKFLKIIGWGLVSLFTLSALVLSTIS
                     IIIGWAVILPIQVCAVFLLIGGVCIFKGKRLGDQISRYKKYCTIINNRNIIPVELIAE
                     NTSKSLNFIIKDVQKMIDKNYFINTYIDKRNNQIVLTNEDFVPPRYEDVAYEVKEEEK
                     TDESNEVDTIINKGMNYLTQIKEANKNIKSESMGDKIVQVEDVTSKIFDVVKHDPSKL
                     TQIQKFMDYYLPTTLKLLNSYHTLEEQGIDRENITTTMESIENTMNTIVVAFENQLDY
                     LFEDEAIDISTDITVLENMLVQEGLTSGKF"
     gene            1058..2215
                     /locus_tag="ACGI59_002983"
     CDS             1058..2215
                     /locus_tag="ACGI59_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HFG9740761.1"
                     /translation="MNNLDDIPVMPTLTLDPFGEESNTNDIDNSDLLMKKDEKDPEEE
                     KLSESERKMVKEFAEKIDITNTNMILQYGAGAQKKVASFSETALKSVKTRDLGEVGDM
                     LTNLVTDLKAFSADEKEQSGFLGIFKKANNKISNLKTKYDSAEVNVDKVSKELQKHQV
                     KILKDIAMLDKMYELNLAYSKELTMYIIAGKQKLKDMKEYEMPKLREKARLSGSTEDA
                     QSVNDMVSLCDRFEKKIHDLELTRMVSLQMAPQIRLVQNSNNLMAEKIQSTIVNTIPL
                     WKNQIVLALGISHLNQAMKAQREVSNMTNELLLKNAETLKMGTIETAKESERGIVDID
                     TIKKTNQSLISTIDEVVKIQHDGRIKRQEAEVELSKIENELKSKLLEFTVK"
     gene            complement(2300..3799)
                     /locus_tag="ACGI59_002984"
     CDS             complement(2300..3799)
                     /locus_tag="ACGI59_002984"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFG9740762.1"
                     /translation="MAFYFDTPSHTFSEYLLVPGFSSTDCIPANVSLKTPVTKFKKGE
                     EADIYMNIPLTSAIMQSVSDDKMAIALAKEGGISFIYGSQTIENEAAMVARVKSHKAG
                     FVVSDSNIKPDNTLKDILDLKEKTGHSTVAVTEDGTSTGKLLGIVASRDYRISRMDLD
                     TKVSEFMTPMSSIVYANKDVTLKEANNIIWDHKLNSLPVLDDNGNLMYMVFRKDYSSH
                     KENPLELLDSSKRYVVGAGINTRDFAERVPALVEAGADVLCIDSSEGFSEWQKITLDF
                     IREKYGDTVKVGAGNVVDREGFLYLAEAGADFVKVGIGGGSICITREQKGIGRGQATS
                     IIEVAQARDEYFEKTGIYIPICSDGGIVYDHHITLALAMGADFIMLGRYFSRFDESPT
                     NKVNINGSYMKEYWGEGSARARNWQRYDLGGDKKLSFEEGVDSYVPYAGSLKDNVTLS
                     LSKVRSTMCNCGALSIPELQKNAKLTLVSSTSIVEGGAHDVMLKDNRNL"
     gene            4395..5819
                     /locus_tag="ACGI59_002985"
     CDS             4395..5819
                     /locus_tag="ACGI59_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HFG9740763.1"
                     /translation="MSSSNQSTIKTSIQRVGKFLSGMVMPNIGAFIAWGLITALFIPT
                     GWMPNEKLSTIGDPMIKYLLPLLIAYTGGKAIAGQRGGVIGAAAAMGVIVGADIPMFI
                     GAMIMGPFAGWVIKKFDKFVDGKIPTGFEMLVNNFSIGIIGMLLAILGFYAIGPAIVA
                     GTALIESGVQFIVSKSLLPLVSVFIEPGKVLFLNNAINHGILGPIGIAEAKEAGKSIM
                     FLLESNPGPGLGVLLAYWMFSRGSVKQSAPGAVIIHFFGGIHEIYFPYILMNPVLILA
                     TMAGGAAGILTFSILGAGLVAAPSPGSIFALMALAPKGGLLPVLAGVAVATVVSFLVA
                     APFVKRASANQSEEDSTSLEEAKAKMSDMKSASKNSEKNIEEKQLEVNEIKKIVFACD
                     AGMGSSAMGASRFKNRIKNLDLNIEITNSSVDNLPDDTQIVVTHNTLVERVAKNNSSV
                     EIVSINNFLNDPNLDTLFKRLESK"
     gene            5845..7983
                     /locus_tag="ACGI59_002986"
     CDS             5845..7983
                     /locus_tag="ACGI59_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFG9740764.1"
                     /translation="MDNLKKKKITSRQKKIILMIVANSKKNIPITISEIAGTLELSSR
                     TVLRDMSGIEKWFDENDFNFVKKPGVGLILEENIENQNFIIELLEEEKIEKEYSKEER
                     NLIILSKLLVSNEPVKSYYFAKILKVSEWVLNNDFALASKWLERFDIELVRKPGLGVY
                     LKGQEKNFREAYVNLIYDSFNEKEILDMVRNISENIQTDKAIEILSENRLLNLMDRCI
                     IRKVEATLTKKLSDLDVNLADSAYIGLVVHISLALQRIKNGENITMDKEFLKELSITE
                     EFKLAKEIVKGMETDFNMDIPVDEVGYITMHIRGAKQRSSSNHKALNLDDIEIMEITN
                     KMIDLAEDEFKISLKNDERLFKDLANHLGPSINRLNMGLEIRNPLLDEIKSKYSYAYD
                     VVEKISRIIKDKLNINSIPESEIGYIAMHFASAIEKNLMMNTNINIVVACPTGIGTSR
                     FLSTKIENKFPNLNILETISAINIDEEYLKEKDVDLIVSTVELNTSLNYICVGPFMSL
                     DDEQIIKEKIKSIAQNKLINLNAKNDTKSKNKVYEQITESMNIGKDILQFLEEIRFEK
                     FESKDLSELIEDSSKIFAKSSEDIISIKESLKERLKISIPYIEESKILLLHCMSERID
                     IMKLSIIKLENRIVLDSNEEIDNVVFMLLPKNSPSYQRQIMSEISGSLIDNIIFTNKI
                     NKFSIEEMALEVKDIVFNFYTNRLKAFIDK"
     gene            8013..8435
                     /locus_tag="ACGI59_002987"
     CDS             8013..8435
                     /locus_tag="ACGI59_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFG9740765.1"
                     /translation="MSKVLNENNIFLGLDSVSKEEAITLAGRKLVENGYVKEEYIPAM
                     LEREKVMTTYMGMGVAIPHGVNEAKKEILSSGIVILQFPNGIDFDGEKAYLLIGIAGV
                     GDEHLEILSNIAVVLDDDLTERLKNSNDKQAFMEAFAN"
     gene            8454..9605
                     /locus_tag="ACGI59_002988"
     CDS             8454..9605
                     /locus_tag="ACGI59_002988"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008926 - mannitol-1-phosphate
                     5-dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0019594 - mannitol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFG9740766.1"
                     /translation="MKKAIQFGAGNIGRGFIGGLLVKSGYHVVFADVNEEILNSINKD
                     KKYTIHIRDVECIDEVIDNISAVSSIKEEIIDEIVQAEIITTAVGPLVLTKIASTIAK
                     GIKARKEKGLTSNLNIIACENAIYASSSLKEEVLKYLNKEEVEYLEMYVGFPNCSVDR
                     IVPPGKNENPLDVTVENFYEWNVEKQGFKGEIPTIVGMNLADNLMAYIERKLFTLNTG
                     HAITAYIGYLKGYKTIEESIKDKFICDIVKSAMVESGEGLIKKYNFDSEVHYKYIDKI
                     LNRFKNPYLNDDVLRVGREPLRKLSDKDRLIKPLMTAKSYGLSVDNLILGIGAALHYN
                     NSEDTQSVELQELIKSIGVKKAVAKIANISNDEELLNNIEKSYIFMKNL"
     regulatory      9711..9812
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            9995..10567
                     /locus_tag="ACGI59_002989"
     CDS             9995..10567
                     /locus_tag="ACGI59_002989"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFG9740767.1"
                     /translation="MKALQEKILREGSVSGNDILKVDSFLNHQIDVAFLNEIGREFKE
                     RFKGEKVDKIFTIEASGIAIASIVSQYFDNAPVVFAKKSESKNLDKDVYETNVYSFTK
                     AREYSVKVSKKYINKGENILIVDDFLANGRAALGLKDLIEQAEANLVGVGIVIEKGFQ
                     AGGALLKANDVRLESLAVVESIDNGTVKFR"
     gene            10631..11281
                     /gene="fsa"
                     /locus_tag="ACGI59_002990"
     CDS             10631..11281
                     /gene="fsa"
                     /locus_tag="ACGI59_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFG9740768.1"
                     /translation="MRFFIDTANIEEIKEANDLGVICGVTTNPSLIAKEGRDFIEVVK
                     EISEIVDGPISAEVISLEHKGMIEEAEKLSKIHKNIVIKIPMTAEGLKAVKVLSSKGI
                     KTNVTLIFSAGQALLAARAGATYVSPFVGRLDDISQNGLDLIEEIVDIFSVNSIEAQI
                     IVASVRNPIHVLQAARMGADIATVPLKVINQMIKHPLTDKGIDSFMKDWEGASLKL"
     gene            11433..14357
                     /locus_tag="ACGI59_002991"
     CDS             11433..14357
                     /locus_tag="ACGI59_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFG9740769.1"
                     /translation="MDKILREIQREDKKNPYTDQELAEMLNVARSEVISVRKKNNILD
                     SRERRKKILIKDISKIVSENPQMSERKITEILIENGYNISRSAVSKLLKEENLNQSKI
                     GIKDKDPASSGKYVKVSKVVEEDGFEELIGIKGSLKEKVNLAKSAIMYPPNGLHTIIY
                     GETGVGKSELATCMYKYAIKNNIKEENSPFIVFNCADYAENPNLLIAQLFGVVKGAYT
                     GADANREGLVEQANNGILFLDEIHRLPAAGQEILFSLIDRGEFRRLGESSSNRKANVL
                     IISATTENPDSNLLQTFRRRIPMVINLPNMSERPKSERYEIIIKFFEREAKRVNKNFI
                     ITKEVMCALMNYKCTGNIGQLKSDIQVTCARAFSKSIFSSDKVIIDLDSLRDYIKSGY
                     YDCNCKNEKYSEFSVEDIYIDISELVSGKNRILSDNEISEIYNYAEKELKVLESKCFS
                     EEELKNAFIEKLDKKFNAIKNNKNLSDRRSRIDWGGQLKESTLEIMDKVIVFIKEQFK
                     HVNQGLYLALAIHIEHAIGRIKDGKTIINPSLDKIKASMPMEYELSRYIMGIVEDLTQ
                     VTFPEDELGYLAYYINKFCYNEESIKDKVKVVIVTHGKVGIEMSKVVNHILGIECTLG
                     IEIALTDSPSEGIEHVLEELQKIEARKGILVLIDMGSLVILGDEVEKRLGIRCKTVNR
                     VDTLLAMEAGKLATIEGKSLDGIIADLKKNKNYAMINTNKFSYRKNEYGKKNVIITLC
                     LSGVGTALNLKEHIEKQIKEYDTLIEVKPVAFLNNNLEEELNDIESEYNIIAICGTID
                     IDYKNVPFISYNEVLGKNGINKVLEHLNSKQPALNIENQLNNLIHEDLILYNFEGISK
                     EYIIDTLVSKLEEGGYVDSKYILSVYKREAMGSVVMASKVAVPHGLPENVMKPAIAIA
                     RLNKPIVWDNKFMVDLVVLLALKENNKKEIRSLFSKINDQHTLEVLLNTEDKNEIKEL
                     LS"
BASE COUNT         5894 a         1580 c         2571 g         4488 t
ORIGIN      
        1 gaaaatgaaa ggattataaa atatgtataa atcagaaaat ttttcaaaaa aatctgtatt
       61 aataaattca tttttaagta aatttttaaa aataattgga tggggtttgg taagcttatt
      121 tacattatca gccttagtac tttcaactat ttccattata attggatggg ctgtaatact
      181 tcctattcaa gtatgtgcag tatttttact tataggagga gtttgtattt ttaaaggtaa
      241 gagattagga gaccaaattt ctcgttataa aaaatattgt acaattataa acaatagaaa
      301 tattattcca gtagaactta ttgcagaaaa cacatctaaa tcattaaatt ttataataaa
      361 agatgtacaa aagatgatag acaaaaacta ctttataaat acttatattg ataaaagaaa
      421 taatcagatt gtgttaacaa atgaagattt tgttccacca agatatgaag atgtagcata
      481 tgaagtcaaa gaagaagaaa agactgatga atccaatgaa gttgatacaa ttataaataa
      541 aggaatgaat tatctaacac aaataaaaga agctaataaa aatattaaat cagaatcaat
      601 gggcgataaa attgtacaag tagaagatgt tacaagtaaa atatttgatg ttgtaaaaca
      661 tgacccatca aaacttactc agatacaaaa atttatggat tattatcttc caaccacttt
      721 aaaactatta aattcatatc atacattgga agaacaagga attgatagag aaaatataac
      781 aacaactatg gaaagtattg aaaatacaat gaatacgatt gtagtagctt ttgaaaatca
      841 attggactat ctgtttgaag atgaagccat tgatatttct acagatatta ctgttttaga
      901 gaatatgtta gtccaagaag gattaacgag tggtaaattt taacttcaat agaaaaaata
      961 ttctgttatt tttaaacaat taattagtca taaagtataa aatgtgtatt atataagtta
     1021 atcagtactt tatgaaggaa gaggagagaa agatacaatg aataatttag atgatatacc
     1081 agttatgcca actttgacac ttgacccatt tggagaagaa tcaaacacta atgatataga
     1141 taattctgac ttattaatga aaaaggatga aaaagaccca gaagaagaaa aactgtctga
     1201 atctgaacgc aagatggtaa aggaatttgc ggaaaaaata gatattacaa atacaaatat
     1261 gattttgcaa tatggagctg gagctcaaaa aaaggtagca agtttttctg aaacagcttt
     1321 aaagagtgta aaaacaagag atttaggaga agttggagat atgcttacaa atctagttac
     1381 tgatttaaaa gcattttcag cagatgaaaa agaacaaagt ggttttttag gaatttttaa
     1441 aaaggcaaat aataaaattt caaatttaaa aactaagtat gatagtgcag aggtaaatgt
     1501 ggataaagta agcaaagagc ttcaaaagca tcaggttaaa atattgaaag atatagcaat
     1561 gcttgataag atgtatgaat tgaacttagc ttactcaaaa gagcttacta tgtatattat
     1621 agcaggaaaa caaaaattaa aggatatgaa agaatatgaa atgccaaagt tgagagaaaa
     1681 agctcgttta tctggttcaa cagaagatgc tcaatctgta aatgatatgg tttcactttg
     1741 tgacagattt gaaaagaaga ttcatgactt agaattaact agaatggtat cgcttcaaat
     1801 ggctcctcag ataagattgg ttcaaaatag taataatctg atggcagaaa agattcaatc
     1861 aactatagta aataccattc cattatggaa aaatcaaata gtgcttgctc ttggaatttc
     1921 tcacttaaat caagctatga aagcacagcg tgaagttagt aatatgacaa atgagctttt
     1981 attaaagaat gcagaaactt tgaagatggg aacaatagaa acagcaaaag aatcagaaag
     2041 aggaattgta gatatagaca caattaagaa aactaatcaa tctctaatat ctactattga
     2101 tgaggtagtt aaaatacaac atgatggtag aataaagaga caagaagcag aagttgaatt
     2161 atcaaaaata gaaaatgaac ttaaaagtaa gcttcttgaa tttacagtta aataaaaatt
     2221 atactaatat aaaaagagac tgtctcaaaa tagagataag tctctttttc attgtttaaa
     2281 tgattaaagt atatttatat tataaatttc tattatcttt taacataaca tcatgagctc
     2341 caccttcaac tatactagta gaagatacaa gtgtaagttt agcatttttt tgaagttcag
     2401 gaatgctaag tgctccacag ttacacatag tagaacgaac tttacttaaa gaaagagtta
     2461 cattgtcttt caaagaacca gcataaggca cataagagtc aacaccttct tcaaaagaaa
     2521 gtttcttatc tccaccaagg tcatatcttt gccagtttct agcacgagca gaaccttctc
     2581 cccaatactc tttcatataa ctaccattta tattaacttt gttagttgga ctctcatcaa
     2641 aacgagagaa gtatctacca agcattataa aatctgcacc catagctaaa gctaaagtta
     2701 tatgatggtc ataaacgata ccaccatcag agcaaattgg aatataaata cctgtttttt
     2761 caaagtattc gtctctagct tgagcaactt caattataga agtagcttgt ccacgtccta
     2821 tacctttttg ttcacgagtt atacatatag aaccaccacc aataccaact ttaacgaagt
     2881 cagcaccagc ttcagctagg taaaggaatc cttcgcggtc aactacattt ccagctccta
     2941 ccttaactgt atcaccatat ttttcacgaa tgaaatcaag tgttattttt tgccactcag
     3001 aaaaaccttc tgaagaatct atacataaaa catcagcacc agcttcaact aaagcaggaa
     3061 cacgttcagc aaagtctcta gtattaatac cagctccaac tacatatctc ttagaagaat
     3121 ctaaaagttc aagtggattt tctttatgag aagagtaatc tttacggaat accatataca
     3181 taagattacc attatcatca agtacaggaa gagaattaag tttgtggtcc catataatat
     3241 tgtttgcttc ctttaaagta acatctttgt tagcataaac tatagatgac ataggagtca
     3301 taaattcact aactttagta tctaaatcca tacgacttat acgataatca cgacttgcaa
     3361 caatacccaa taatttacca gttgaagttc catcttcagt aacagctact gtagaatgtc
     3421 cagttttttc ttttaagtca agtatgtctt ttaatgtgtt atcaggttta atatttgaat
     3481 cacttacaac aaaaccagct ttgtgggatt taacacgtgc taccatagca gcttcatttt
     3541 caatagtttg agaaccgtat ataaatgaaa taccaccttc tttagcaagt gcaatagcca
     3601 ttttgtcatc agaaacagat tgcataatag cagaagtcaa aggtatattc atataaatat
     3661 ctgcttcctc gcccttttta aatttagtaa caggtgtttt taaacttaca ttagcaggta
     3721 tacaatcagt agaagagaaa cctggtacaa gaaggtattc actgaaagta tgtgatggtg
     3781 tatcaaaata aaacgccatt ttttaatcac tccttatatt tttatacttt ttataattga
     3841 tagtattaat tataacttat tctttaattt tattaaagta ttttgtcaaa tattgagatt
     3901 tttgaaaaat ttaaactatt ttaattaaat ttgtttaata tatattgcct tatagggtaa
     3961 agttgtatat taaataatgt ttagtgttta atagtagcta atttaaaatt aatctttagt
     4021 gattcatata attacatttg tatagtttac attgataata ttactagaga gtgcataaac
     4081 ctcaaaaata cagataaatt aaatctatat tagtatatcc aaaagtacat ttaataaatt
     4141 tatttaactg aattattatt ttcatttcta ttaaatttaa atattttata aaaatagtat
     4201 tatttacaaa atcatatact ccaaaatact acataaataa aattaaacaa aaactaaaat
     4261 gacaaatgtc attatctaaa gtgacataga aacggtttct taaagttgtt actataaaat
     4321 ataaactcta taataaaagt ataacaaaag ggaatcatat cgatatagaa tataattcaa
     4381 gaggaggtaa aattatgagt agttctaatc aatcgacaat aaagacatct atacagcgag
     4441 tagggaaatt tttaagtggt atggtaatgc caaatatagg agcttttata gcatggggtc
     4501 ttataacagc attatttata ccaacaggat ggatgcctaa tgaaaaattg agtactatag
     4561 gtgacccaat gataaaatac cttttaccac tattgatagc ttatactggt ggtaaagcaa
     4621 tagcaggaca aagaggtggc gtaataggtg cagcagcagc tatgggagta attgttggtg
     4681 cagatatacc aatgtttatt ggagcaatga taatgggacc atttgcagga tgggtaatta
     4741 agaagtttga taaatttgta gatggaaaga taccaacagg atttgaaatg ttagtaaata
     4801 acttttcaat aggtataata ggtatgcttc ttgcaatact aggattttat gcaattggac
     4861 cagcaatagt agcagggaca gctcttatag aatctggagt tcaatttata gttagcaaaa
     4921 gtttattgcc tctagtttca gtatttatag aacctggaaa agtattattc ttaaacaatg
     4981 caataaatca tggtatatta gggcctatag gtatagctga ggcaaaagaa gcaggtaaat
     5041 ctataatgtt cttactagaa agtaaccctg gaccaggttt aggagttcta ttagcatatt
     5101 ggatgtttag tagaggaagt gtaaaacaat cagcaccagg agctgtaata atacatttct
     5161 ttggtggtat ccatgaaatt tacttcccat atattttaat gaatccagtt ttaatattgg
     5221 ctacaatggc aggtggagca gcaggaatat tgactttttc aatattagga gctgggcttg
     5281 tagcagcacc atcaccagga agtatatttg cacttatggc actagctcca aagggaggat
     5341 tacttccagt attagcagga gttgcagtgg caacagttgt atcattctta gtggcagcac
     5401 catttgttaa gagagcttca gcaaatcaaa gtgaagaaga cagtacttct ttagaagaag
     5461 ctaaagcaaa aatgagtgat atgaaatctg caagtaagaa ctctgagaaa aatattgagg
     5521 aaaaacagct tgaagtaaat gagattaaga agattgtatt tgcatgtgat gcaggaatgg
     5581 gttcgagtgc aatgggagct tccagattta aaaatagaat aaaaaactta gacttaaata
     5641 tagaaattac aaattcatct gttgataatc ttccagatga tacacaaata gtagtaactc
     5701 acaatacatt agttgaaaga gttgctaaga ataattcaag tgttgagata gtttcaataa
     5761 acaatttttt aaatgaccca aatttagata cattgtttaa aagattagaa tctaaataaa
     5821 aataaaaagt accactaatt aggagtggat aatttgaaaa aaaagaagat tacatctagg
     5881 caaaaaaaga taatactaat gatagtagca aattcaaaga aaaacattcc aataactatt
     5941 tctgagattg cagggacttt agagttaagt tcaagaactg tacttagaga tatgtctgga
     6001 attgaaaaat ggtttgatga aaatgatttt aactttgtaa aaaaaccagg agtgggattg
     6061 attttagagg aaaatataga aaatcaaaac tttataattg agttgttaga agaagaaaaa
     6121 atagaaaaag aatacagcaa ggaagaaaga aatttaatta tattatcaaa gctactagtt
     6181 tctaacgagc cagtaaaatc ctattatttt gcaaaaatat taaaggtatc tgaatgggta
     6241 ctgaataatg attttgcctt agcaagtaaa tggttggaaa gatttgatat tgagcttgtt
     6301 agaaaaccag ggctcggtgt ctacttaaaa ggtcaagaaa aaaactttag agaggcatat
     6361 gtaaatctta tatacgattc attcaatgaa aaagaaatac tagatatggt cagaaatata
     6421 agtgaaaata tacaaacaga taaagcaata gaaattttaa gtgaaaatag acttttaaat
     6481 ttaatggaca ggtgcataat aagaaaagtt gaagctacat taacaaagaa gctttcagat
     6541 ttagatgtaa atttagctga tagtgcatat atagggcttg tagttcatat ttcattagcc
     6601 ttacagagga taaaaaatgg tgaaaatata actatggata aagagtttct aaaagaatta
     6661 tctataacag aagagtttaa attagctaaa gaaatcgtaa aaggtatgga aacagatttt
     6721 aacatggata taccagtaga tgaggttgga tatataacta tgcatataag aggtgcaaag
     6781 caaaggtcat cttcaaacca taaggcttta aacttagatg atatagaaat aatggaaatt
     6841 acaaataaga tgatagattt ggcggaagat gaatttaaaa taagtttaaa aaatgacgag
     6901 aggttattta aagacctagc aaatcactta gggccttcta taaataggtt gaatatggga
     6961 ttagaaataa gaaatcctct tttagatgaa ataaaaagca agtattcata tgcctatgat
     7021 gtagttgaaa agatttctag aattataaaa gataaactaa atataaattc aatacctgaa
     7081 tcagaaatag gatatattgc aatgcacttt gcatcagcca tagaaaaaaa tcttatgatg
     7141 aatactaata taaatattgt tgtggcatgt cccactggaa taggtacttc aagattctta
     7201 tcaactaaga ttgaaaataa atttcctaat ttaaatatac ttgaaaccat atcagctata
     7261 aacatagatg aagagtattt aaaggaaaaa gatgttgatt taatagtgtc tacagttgag
     7321 ttaaatacaa gtttaaatta tatatgtgta ggtccattta tgagtttaga tgatgaacag
     7381 ataatcaagg aaaaaataaa atctatagct caaaataaat taatcaattt aaatgcgaaa
     7441 aatgatacta agagtaagaa taaagtatat gaacagataa cagaaagtat gaatatagga
     7501 aaagatattc ttcaattttt agaggaaatt agatttgaaa aatttgaaag taaagattta
     7561 agtgagctta ttgaagactc atcaaaaata tttgcaaaat ctagtgaaga tataatctct
     7621 ataaaagaat cgttaaaaga aagattaaaa atttcaattc catatataga agaatcaaaa
     7681 attctattgt tacattgtat gagtgaaaga atagatatta tgaagttatc aataataaaa
     7741 ctagagaata gaatcgtgct agattcaaat gaagaaatcg acaatgtagt ttttatgtta
     7801 cttcctaaaa attcaccaag ttatcaaaga caaataatga gtgaaataag tggttcatta
     7861 atagacaata taatttttac taataagata aataaattct ctatagaaga gatggcatta
     7921 gaggtaaaag atatagtttt taatttttat acaaacagat taaaagcatt tatagataaa
     7981 taaaattaat attaaaaagg aaggttgata ctatgagtaa agtattgaat gagaataata
     8041 tatttttagg gttagatagt gtttcaaagg aagaagctat aactttagca ggaagaaaat
     8101 tagttgaaaa tggatatgta aaagaagaat atataccagc aatgttagaa agagaaaaag
     8161 taatgactac atatatggga atgggagttg ctattccaca tggtgtaaat gaagcaaaaa
     8221 aagaaatatt atcttctgga atagttatat tacaattccc taatggtata gattttgatg
     8281 gagaaaaagc atatttatta ataggaatag ctggtgttgg agatgaacat ttagaaatac
     8341 tatctaatat agcagtagtt ttagatgatg acttaacaga aagactaaaa aattcaaatg
     8401 acaaacaagc ttttatggaa gcttttgcca actaatctta ggagggattt aaaatgaaaa
     8461 aggcaattca gtttggagca ggaaatattg gaagaggttt tattggaggc ttgttagtta
     8521 agtcgggtta tcatgtagta tttgctgatg taaatgagga gatattaaac tcaataaaca
     8581 aagataaaaa gtatactata cacataagag atgtagaatg tatagatgaa gtaattgata
     8641 atataagtgc ggtttcatct ataaaagaag aaataataga tgagattgta caggctgaaa
     8701 taataactac agcagttgga cctttagttt tgactaaaat agcctctaca attgcaaaag
     8761 gtataaaggc tagaaaagaa aagggattaa caagtaatct aaatataata gcatgtgaaa
     8821 atgcaatata cgcaagttct tctttaaaag aagaggtact taagtattta aataaggaag
     8881 aagtagaata tttagagatg tatgttggtt ttccaaactg ttcagttgat agaatagttc
     8941 ctcctggtaa aaacgaaaat cctcttgatg taacagttga aaatttttat gagtggaatg
     9001 ttgaaaaaca aggatttaaa ggggaaatac caactatagt tggtatgaat ttagctgata
     9061 atcttatggc ttatatagaa cgtaaattgt ttacactgaa tacaggtcat gctataacag
     9121 cttatatagg atatttaaaa ggatataaga ctatagaaga gagtataaaa gataaattta
     9181 tctgtgatat agtcaaaagt gcaatggtag aaagtggaga aggattgatt aaaaaataca
     9241 attttgactc agaagtgcac tataaatata ttgataaaat tttaaataga tttaaaaatc
     9301 catatcttaa tgatgatgta ttaagagtcg gaagggaacc attaagaaaa ctaagtgata
     9361 aagatagatt gattaaacct ttaatgacag caaaatcata tggattatca gttgataatt
     9421 tgattcttgg aataggtgca gcgcttcatt ataataatag tgaagatact caaagtgtag
     9481 aattacaaga gttgataaag tctattggag taaaaaaagc cgttgctaaa atagctaata
     9541 taagcaatga cgaagaactt ttaaacaata tcgaaaaaag ttatatattt atgaaaaatt
     9601 tataaattta cttgaaaaaa agtagttctg ataaagtaac tataatatat tattctacac
     9661 agtttgaatt atttgttgaa taatagagaa ttataatata taattaaaag gaataaatta
     9721 atatatttac ctgtatatgt ttgataatat ggttcaaatg tttctactaa gctaccatta
     9781 atagcttgac tacaggagtg aagaagatat agtttatact tattttaaat tatatattaa
     9841 gttaattact ttgaatttta agctcctgtc tatgatatag acaggagctt ttttagtatt
     9901 ttaatataaa atacttacaa gaatagatag caaaattaga acaaatataa tttttttatt
     9961 aatataattt tagaaagggc taggtggaag ttcaatgaag gctttacaag aaaagatatt
    10021 gagagaaggc agtgtttcag gaaatgatat actaaaagtt gatagttttt taaaccatca
    10081 aattgatgta gcttttttaa atgaaatagg aagagaattt aaagagagat ttaaaggaga
    10141 aaaggtggat aagatattta caatagaggc ttcaggaata gctatagcat caattgtttc
    10201 acagtatttt gataatgcac ctgtagtatt tgcaaaaaaa tcagaatcta aaaatttgga
    10261 taaagatgta tatgaaacta atgtatattc atttactaag gcaagagagt attcagttaa
    10321 agtttctaaa aagtacataa acaaaggcga aaatatattg atagtggatg attttttggc
    10381 aaatggaaga gctgctcttg gacttaaaga tttaattgag caagcagaag ctaacttagt
    10441 tggagtagga attgtcattg aaaaaggatt tcaagctgga ggagcacttt taaaggctaa
    10501 tgatgtaaga ttagagtctt tggcagtagt tgaatctata gataatggaa ctgtaaaatt
    10561 taggtaattt atgatattat atacttgtgt ttagcacata ttttaactat gtaattatgg
    10621 aggatttttt atgagatttt ttatagacac agccaatatt gaagaaataa aagaagcaaa
    10681 tgatttaggt gtaatctgtg gtgtaactac taatccgtct ctaatagcta aagaaggtag
    10741 agattttata gaggtagtta aagaaataag tgaaatagta gatggaccaa taagtgcaga
    10801 agtaataagt ttagaacata aaggtatgat agaggaagca gaaaaattat ctaagataca
    10861 taaaaatata gttataaaga tacctatgac agcagaaggt ctaaaagcgg tgaaagtact
    10921 atcaagtaaa ggtataaaga ctaatgtaac gcttatattc tctgctggac aagctctttt
    10981 agctgctaga gctggagcta catatgtaag tccttttgtt ggtagattgg atgatatatc
    11041 acaaaatggt cttgatttaa ttgaagaaat cgttgatata tttagtgtaa atagtattga
    11101 ggctcaaata atagttgcta gtgttagaaa tccaatacat gttttacaag ctgctagaat
    11161 gggagcagat atagcaacag ttcctcttaa agttataaac caaatgatta agcatccttt
    11221 gacagacaaa ggaatagata gctttatgaa ggattgggaa ggtgcttctt taaaattata
    11281 attataaact acgctttata agtatattaa agaggttatt atggaaattc taagtaataa
    11341 ggctaggcta ttttgcctag tcttattttg gtgtataata tattgaaaca tattattgaa
    11401 atagattaat tttttaagtg aggaggcgaa aaatggataa aatattaaga gagatacaaa
    11461 gagaagataa aaaaaatcca tatactgacc aagaacttgc tgaaatgtta aatgtagcta
    11521 ggtctgaagt gatatcagtt aggaaaaaaa ataatatttt agattcgaga gaaagaagaa
    11581 aaaaaatatt aataaaagat atatcaaaaa ttgtaagtga aaatcctcaa atgtctgaaa
    11641 gaaaaataac tgaaatatta atagaaaatg gatataatat atcgagaagt gcagtttcaa
    11701 aattattaaa agaggaaaat ttaaatcagt caaagattgg gattaaggat aaagacccag
    11761 catcatcagg aaagtatgtg aaggtgagta aagttgtaga agaagatgga tttgaagaat
    11821 tgataggtat aaagggaagt ttgaaagaaa aggtaaatct agctaagtca gcaataatgt
    11881 atcctcctaa tggccttcat actataatat atggagaaac aggagtaggt aaaagtgaat
    11941 tagctacatg tatgtataaa tatgctataa aaaacaacat taaagaagag aacagcccat
    12001 ttatagtgtt taactgtgca gactatgcag aaaatcctaa tctattgata gctcaactct
    12061 ttggggtagt aaaaggtgca tatacaggag cagatgcaaa tagagaaggt cttgtagaac
    12121 aagctaataa tggaatattg tttttagatg aaattcatag attgcctgct gctgggcaag
    12181 aaatcttatt ttcattaata gatagaggag aatttagaag gttaggagaa agtagttcta
    12241 atagaaaggc aaatgttctc ataataagtg ctacaacaga aaatccagat tctaatttac
    12301 ttcaaacatt tagaagacgt ataccaatgg taataaattt acctaatatg agtgaaagac
    12361 caaaatctga aagatatgaa ataataataa agttttttga aagagaagca aagcgagtaa
    12421 acaagaattt tataataact aaagaagtta tgtgtgcact tatgaattat aagtgtactg
    12481 gaaatatagg tcagttaaag agtgatatac aagtaacttg tgctagagct tttagtaaat
    12541 cgatattttc tagcgataaa gtaattatag atttagatag tttaagggat tatataaaaa
    12601 gtggatatta tgattgtaat tgtaaaaatg aaaagtattc tgagttttca gttgaggata
    12661 tatatataga tattagtgaa ttggtgagtg gaaaaaatag aattttatct gataatgaga
    12721 taagcgagat atataattat gcagaaaaag agcttaaggt tttggaaagt aagtgctttt
    12781 cagaagaaga gttaaaaaat gcatttatag aaaagttaga taagaagttt aatgctatta
    12841 aaaacaataa aaatttatct gatagaagaa gtcgaataga ctggggagga caattaaaag
    12901 agtctacact ggagataatg gataaggtaa ttgtatttat taaggagcag tttaagcatg
    12961 taaatcaagg tctgtacttg gctctagcaa ttcatattga gcatgctata ggtaggataa
    13021 aagatggaaa aactataata aatccgagtt tagataaaat aaaagcatca atgccaatgg
    13081 aatatgagtt gtcaagatat ataatgggta tagtagaaga tttgacacaa gtaacatttc
    13141 cagaagatga gcttggatat ttggcttatt atataaataa attttgttat aatgaggaaa
    13201 gtataaaaga taaagttaaa gtagtaatag ttacgcatgg taaagttgga attgaaatgt
    13261 ctaaagttgt aaatcatatt ttaggtatag aatgtacact aggtatagaa atagcactta
    13321 cagattcacc atcagagggg atagagcatg tattagaaga acttcaaaag atagaagcta
    13381 gaaaaggtat tctagttcta attgatatgg gttctttggt aattcttggt gatgaggttg
    13441 aaaaaaggct tggaattaga tgtaaaactg taaatagagt agatacatta ttggcaatgg
    13501 aagcaggaaa acttgcaaca attgaaggaa aaagtttaga tggtataata gctgatttaa
    13561 agaaaaataa gaattatgct atgataaata caaataaatt tagttacaga aaaaacgagt
    13621 atggaaagaa aaatgtaatc attactttat gtttgagtgg tgtaggtaca gctttaaatt
    13681 taaaagagca tatagaaaaa caaattaagg aatatgacac tttaattgaa gtaaagccag
    13741 ttgctttttt aaataataat ttagaagaag agttaaatga tattgaatct gaatataata
    13801 ttattgctat ctgtggaact attgatatag attataaaaa cgtgcctttt atttcttata
    13861 atgaggtact tggtaaaaat ggtattaata aagtattaga gcatttaaat agtaaacaac
    13921 cagctttaaa tattgaaaac cagttgaata atttaataca tgaagatttg attttataca
    13981 attttgaagg aatttcaaag gagtatataa ttgatactct tgtttcaaaa cttgaagaag
    14041 gtggatatgt tgactcaaag tatatattaa gtgtatataa aagagaagca atgggaagtg
    14101 ttgtaatggc ctctaaggta gccgtgccac atggacttcc agaaaacgtt atgaagcctg
    14161 caatagcaat cgcgaggttg aataaaccta tagtatggga caataaattt atggtggatt
    14221 tggtggtact actggcactt aaagaaaata ataagaaaga aatacgaagc ttattttcaa
    14281 agattaatga ccagcatact ttagaagttt tattaaatac tgaagataaa aatgaaataa
    14341 aagaactttt aagctaatta ttagtaccga gaagaaagac aaaaaaattt gctttagtat
    14401 aaaatttaaa actactcaat tgctaagtag ttaattactc tatataaggc tagttctatg
    14461 aaatttattt atatgatttt atagaattag cttttttctt ggtaaataaa atatataaat
    14521 ttatttatgt aaa
//
LOCUS       DAYAPA010000112        14408 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.113, whole genome shotgun sequence.
ACCESSION   DAYAPA010000112 DAYAPA010000000
VERSION     DAYAPA010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14408
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.113"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(43..897)
                     /locus_tag="ACGI59_002992"
     CDS             complement(43..897)
                     /locus_tag="ACGI59_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-ADP-ribose hydrolase"
                     /protein_id="HFG9740770.1"
                     /translation="MKWRDYADDVNLFEDFDKTIKPLTDEQRRKNINTLIAYFSKEVP
                     SKIYNLSNDEIKPRDILRGLLNVYPPKEIAPEILNMLHNLLLIECEERELVDVNDIEE
                     IEEGIAIWRGNITNLRADAIVNAANNKLLGCLQPLHLCVDNEIHSCAGPRLREDCDKI
                     IKKQGHLEYTGDAKITRGYCLPAKFVVHTVGPIVSGGQPSKEQEKQLLHCYKSCLNTI
                     KEIDEIKNIVFCGISTGVFGYPKKEAANLAVSRVRLWLKENPEKNLKVVFNVFTEEEE
                     EKYRRIFK"
     gene            complement(1220..2548)
                     /locus_tag="ACGI59_002993"
     CDS             complement(1220..2548)
                     /locus_tag="ACGI59_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HFG9740771.1"
                     /translation="MEKFSVVIAGGGSTYTPEIILMLLDNLDRLPLRAIKLYDNDEER
                     QNKVAKACEILIKEKDPNIEYLATTCPKEAYTDVDFCLAHIRVGKLEMRELDEKIPLK
                     HGVVGQETCGPGGIAYGMRSIEGVLENIDYMEKYSPNCWMLNYSNPASIIAEAVRRLR
                     PNSRVINICDMPIGMEHNIARIAGLKSRKHMDIRYFGLNHFGWYTSIKDKKGNELLPK
                     LVEHIKEYGFINGEEGMKNDKKDSWFETNLFTKEIVKTDLTTIPSSYLKYYLFPDYVV
                     NHSDVNYTRANEVIDGREKEVFGSCALIEKQGHSKGSGLKIGIHAEFIVDLATALAFN
                     TQERMLLIVQNNGAIENIQDDAMVEIPCIVGKDGYEPLSIGKIPTFQKGLIEQQLAVE
                     KLVVDAWIEKSYNKLWQALMLSKTVPSATVAKELLDDLIEANKNYWPKLN"
     gene            complement(2696..3025)
                     /locus_tag="ACGI59_002994"
     CDS             complement(2696..3025)
                     /locus_tag="ACGI59_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFG9740772.1"
                     /translation="MVPEMEEKVFSIISEAGDAKSDVMMSLKEIKKGDYNKAKHLLNS
                     ASEKIQTASKHHLELLSNAMNSEDSGTDFLVVHSEDHYSNALFAHSLVSELVDIFEMM
                     DPRINKN"
     gene            complement(3049..4338)
                     /locus_tag="ACGI59_002995"
     CDS             complement(3049..4338)
                     /locus_tag="ACGI59_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFG9740773.1"
                     /translation="MEALQNNLRRFLLPIAQKIEKQRHLQAIKEGMISITPIIIVGSL
                     SMLFMALNNMLPEGSAKTLLSENMDTLLIPNKFTMSLLSIYSAFFIAQALAKKYNLNH
                     VEIGMTAVVAQLVVCGQVVDGVLDTSYLDAQGLFVSILVALLVVDITKFMNDKNLVIR
                     FPKEVPSVVNKSFRNLTPMIVCIMLFTAIAAITKNVSGQPLPAIIMNFLAPAISSVDN
                     VFAVTIILFITQLLWFFGLHGAAITSSIWMPIAATYMAENATLIAAGGDPKYVFTIGF
                     YYGFLQVTGSGITLGLVYLMSRSKCKSFNSMGKVVILPSLFGINEPVIFGTPIVMNPY
                     MFVPFVFGPVLVGALNFMALKVGLVGLPIAEPPGFLPPGVAAFLMTLDWKAIVLVFAS
                     IILMTLIYYPFFKIMEKEELNKNAELATTLDDDSFDF"
     gene            complement(4354..4680)
                     /locus_tag="ACGI59_002996"
     CDS             complement(4354..4680)
                     /locus_tag="ACGI59_002996"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFG9740774.1"
                     /translation="MNILLVCANGASTGVLVEKMKSFCSEHEKLKTKTINIEATSFEN
                     LKSYIEANDTDVVLVAPQIRFKEDDVVEACKNYKIAVGLIDTKHYGRMDAPSVMKSAI
                     ELYKNR"
     gene            complement(4815..5444)
                     /locus_tag="ACGI59_002997"
     CDS             complement(4815..5444)
                     /locus_tag="ACGI59_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9740775.1"
                     /translation="MQDKILRDKKANDPWARMTTRNGFSADEIISSFQKAIRRNMVEE
                     ACEFAYELYISSPELEDKLWRRILTISVEDIGMGDPSAVIIINNLNQVRKEYSYADGD
                     RPLFFIHAIRYLCACEKDRSSDLLKNIIIKGFAMGKVPEVMDVALDKHTYRGLEMGRD
                     SFHFLNEASIVIPEKEVDNDYKERYLKILEKYKQEEVIDTAFKFNHWQY"
     gene            complement(5659..8316)
                     /locus_tag="ACGI59_002998"
     CDS             complement(5659..8316)
                     /locus_tag="ACGI59_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFG9740776.1"
                     /translation="MIYKKDKVMKVLESSYRENEKGITANQLSEKTGIARNNISTYLN
                     QLFNEGMVTKIKGRPVYYIPANLNLNKNELLDTQSELLGIKKGTKVENKIKKDSFETL
                     IGKEHSLRPIIEKCKVAMLYPTNGLHTIIYGETGVGKSMIARYMYDYSIDSGIRKNKA
                     PFVTFNCADYANNAQLLMGHIFGVEQGAYTGAESSRKGLLEIANGGILFLDEIHRLPA
                     EGQEMLFTFIDKGTFKRLGDATKERESKVLIICATTENPTSTLLDTFNRRIPMKIEIP
                     SLRDRTMIERMELVKNFFKEESKSISISIKVHKEIIKSLLLYDCKNNVGQLQNDIKLA
                     VANGYLRYKRDNKEPVYIEKKYFDKSINEVSDDYREKSIKADEVITHGIEYFVFTSLG
                     EEEVINYNDINIINAVNTNLNKSVEMNIADASLFNNVKFKSMCESIRQIIKEDEEIDI
                     SESIFKSMAIYIKSVLDKSSSTNNIDLNQIRRNNRSEFKTALKIVGIIENEFDLFLSI
                     ETVAYITLFIVQAKQDEQNKIVNNINIIVAMHGETTASSMVKAVGDIIGKCSAISFDM
                     KLDKSYDEVIVDFKNLINNIDNKDVLLFTDMGSLNSFDEIIKKEKKCGVRVIPMVTTL
                     TVLEAVQKANMGLPLNDVYNSITNTRKYYFGTNEIQNKENLSKTIIIASHVSEGVDNK
                     TRKILEEKMSRYLDGIDIISVPYKTEKDLSLNITKLKESSNIVAVINEQRINIRGIDY
                     ISKKDIDKDENINKLKNIIKISIGYDDVVEGLKTSLKSSNYNRIFKDIKYVSDELFLV
                     FNIEKKYDKVIGLMMHLAFMVDGLIGNTREIEKLDKEKTLDYHKSLSKIKDIVSQLDK
                     KYNIEINEKECYQILLILEYAEIIEKDYQ"
     gene            complement(8675..9619)
                     /locus_tag="ACGI59_002999"
     CDS             complement(8675..9619)
                     /locus_tag="ACGI59_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9740777.1"
                     /translation="MNREKILEIKNLKQYFHLDKSTTVKAVDDISFDIYKGEIFGLVG
                     ESGSGKSTTGKTIIRLHESTGGEVIYKGNCISDKKTYKFIKKDVNKSMQIIFQDSTSS
                     LNPRMTIADIISEPLKIQGICKNKTDRMNKVYEMLKLVGLDRSYANKYPSDFSGGQRQ
                     RIGIARALSVDPEFIIADEPIASLDVSIQAQIVNLFKKLQQEKNLTCLFIAHDLSMVR
                     HISDRIGVMYNGKLVELADSNELYNNPIHPYTKSLLSAIPVPDPRYAKSRNRIEYNSN
                     GYDCSNEKSLSWIDVSDGHFVYSSKSEINKYQQNLKVV"
     gene            complement(9612..10637)
                     /locus_tag="ACGI59_003000"
     CDS             complement(9612..10637)
                     /locus_tag="ACGI59_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9740778.1"
                     /translation="MEHLLEVNNLSVSFKVEEGEVQAVRNVSFNLKKGETLAIVGESG
                     CGKSVLCKSLMRILPYNGYIKNGEVLLKSSDLVKKSEKEMEDIRGKNISMIFQDPMTS
                     LNPTISIGKQIAEAVIIHQGISKSEAKKRAIELIELVGIDNPEKRFKQFPHHFSGGMR
                     QRIVIAIALACNPDVLIADEPTTALDVTIQAQIIDLIKDLQHKIGLSIIFITHDLGVV
                     ATIADRIAVMYAGKIVEIGTVEDIFYDPRHPYTWGLLGSLPTLDSQDDYLYNIPGMPP
                     NLLNPPKGDAFAIRNKNALKIDYEKEPPMFKINDTHSAATWLLHPDAPEVDVPVRVNC
                     GRVISNE"
     gene            complement(10712..12280)
                     /locus_tag="ACGI59_003001"
     CDS             complement(10712..12280)
                     /locus_tag="ACGI59_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFG9740779.1"
                     /translation="MKLKKLKVLSLVMILSLMAGCSSGGDKDKKADTPKDGKVLVYGS
                     NDYTSINPALYEHGEINSLIFNGLTAHDENNKVVPCLAKDWKFDEATNTYTFNLRDDV
                     KWHDGEKFTANDVKFTIETIMNPDNASEIASNYEDITKIDVVNDNTIKITLKAPNTAM
                     LDYLTVGVLPKHALEGKDIATDEFNQKPIGTGPFKLEKWDKGQSITLVKNSDYFVKEP
                     GLDKVVFKIVPDDKAKAMQLKSGELDLAQITPKDMSNFEKDEKNFKVNIMKTADYRGI
                     LYNFNSKFFKDKKAKGLPNALSYAIDRKAIVDSVLLGHGVPAYSPLQMGPYNNPDIEK
                     FEYNPEKAKQEIEKLGWKLGSDGIYEKEGTKLAFEITAGESDQVRVDMAKICAQQLKE
                     IGVDAKAVVVTETDWANQDAHLIGWGSPFDPDDHTYKVFGTDKGANYSAYSNPTIDKI
                     LQKARETEDKDEKLKLYKQFQVEMTKDMPYTFIAYIDAIYVGKPNIKGLTPDTVLGHH
                     GVGIFWNIADWTIE"
     gene            complement(12393..13304)
                     /locus_tag="ACGI59_003002"
     CDS             complement(12393..13304)
                     /locus_tag="ACGI59_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9740780.1"
                     /translation="MKPQYSDFEIVGENYILVAEDEHIETKKTLSKKIKELPYISIII
                     LSIIVIGSVFSSFIMTHEPTYMDLASSNLAPNKTFLFGTDSMGRDIYSMIWYGGKISL
                     FIGLFSTVISTVIGIVYGSISGSASELVDDAMMRFTEIILSIPSILIIIFVQAILGNS
                     NPVSMSIVIGITSWMNISKIVRTEVRQIKNSEYILAAKSMGGGFFYVLKQHLLPNFVA
                     SIMFMVVTNIGAAIGTESTLSFLGIGLPIEIVSWGSMLSLSEEALLSNRWWIILIPGI
                     FLVTTLVCITNIGNYIRKNNNKKSSNL"
     gene            complement(13288..14274)
                     /locus_tag="ACGI59_003003"
     CDS             complement(13288..14274)
                     /locus_tag="ACGI59_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9740781.1"
                     /translation="MLKDILKKLLQFILVMFLLSFIVFYMARLAPGDPLISYYGDGVE
                     RMSTQEKENAMKKLGLDEPIYSQYIKWISNASKGEFGISFKYKQNVTSVINDVYINTI
                     ILGGSGYILTFVLALLLGIFCTLHEDKLIDRIICKLGTITNCIPSFWVALVLILIFSI
                     NLSILPSSGAYSMGEESSILSRISHLILPLTVLILSHLWYYTYMIRNKLLEEIREDYV
                     LLCKAKGLNNRTIVFKHCLRNIMPSYISIMAISIPHILGGTYVVEKVFSYPGLGTLCF
                     ESAKYHDYNMLLVLCLITGALVVFGNMLAQIINNKIDPRMKYDRGDTNEATI"
BASE COUNT         4458 a         2592 c         1565 g         5793 t
ORIGIN      
        1 tgtattatat aataagtatt tttaggattc tgttacataa tattatttaa atattcttct
       61 atatttctct tcttcttctt cagtaaacac attaaataca acttttaaat ttttttcagg
      121 attttctttt agccatagtc taactctact tacagcaaga ttagctgcct ctttttttgg
      181 ataaccaaat acacctgtag aaattccaca aaaaacaata ttttttattt catcaatttc
      241 ttttattgta tttaaacatg atttataaca atgaagcagt tgcttttctt gctctttact
      301 tggctgacca ccagatacaa taggtccaac agtatgtact acaaattttg caggtaaaca
      361 ataacctcta gttatcttag catctccagt atattctaaa tgcccttgtt tttttattat
      421 tttgtcacaa tcttctctaa gtctaggacc tgcgcatgaa tgtatctcat tatccacgca
      481 tagatgtaat ggttgtaaac accctaataa cttgttattt gcagcattta ctattgcatc
      541 tgctcttaaa ttcgttatat tacctctcca tatggcaatc ccttcctcta tttcttctat
      601 atcatttaca tctactaact ctctttcttc acattcaatc aacaataaat tatgtaacat
      661 attaagtatc tctggagcaa tttcttttgg tggatataca ttcaaaagtc ctctaagtat
      721 atctctaggc ttaatttcat catttgataa attgtatatt ttagaaggaa cttcttttga
      781 aaaatatgca attaaagtgt ttatattttt tcttctttgt tcgtcagtta gaggcttgat
      841 agttttatca aaatcctcaa ataaatttac atcatcagca taatctctcc acttcatagc
      901 catctctcca ttcttgtatt ttagacttaa aaataattat gtatttatgt acatatttat
      961 ttatttctat aaacacctac ataaatcctg ttaaaagtgt aaatttttta ttatgcaact
     1021 tctctggtta tttctattga ttgatagtaa attattgtac ctaaatttaa tccaactaac
     1081 aagtaactaa acctactttc ctaactgcaa aatacactat gtttgccact tgtatctcat
     1141 ttgtaaataa ataaagtccc tacaaataca ttaactaggc tatgtactaa actgtaggaa
     1201 ctttaaaaat atattattct taattaagtt ttggccaata atttttattt gcttctatta
     1261 aatcatctag taattctttt gcaactgttg cactaggaac tgtcttagat agcataagtg
     1321 cttgccacaa tttattataa gacttctcaa tccaagcatc aactactaat ttttcaactg
     1381 ctaattgttg ttctattaaa cctttttgga atgttggtat tttccctatt gataatggtt
     1441 catacccgtc ttttccaact atacatggaa tttcaaccat agcatcatct tgtatatttt
     1501 ctatagctcc attattttga actatcaata acatacgctc ctgtgtatta aaagctagag
     1561 ctgtagctaa atccactata aactctgcat gtattcctat cttaagccca ctgcccttag
     1621 aatgtccttg cttttctatc aatgcacatg aaccaaaaac ttctttttct cttccatcaa
     1681 taacttcatt ggcacgagta taatttacat cagaatgatt tacaacataa tctggaaata
     1741 gatagtactt taagtaacta cttggtattg tagttaaatc tgtttttact atttctttag
     1801 taaataaatt agtttcaaac caactatctt tcttgtcatt tttcattcct tcttcaccat
     1861 ttataaaacc atattctttt atatgttcta ctaattttgg taaaagttca tttccttttt
     1921 tatctttaat tgaagtatac caaccaaagt ggtttaatcc aaagtatctt atatccatat
     1981 gtttacgaga tttgagtcct gcaatccttg ctatattatg ttccatacct attggcatat
     2041 cacaaatatt tattacccta gaattaggtc tcaaccttct aacagcttcc gctattattg
     2101 atgctggatt agaataattt aacatccaac aatttggaga atacttttcc atataatcta
     2161 tgttttcaag aacaccttct atagacctca ttccataagc tattccacct ggtccacaag
     2221 tttcttgacc aactacccca tgttttagag gtatcttttc atctagttct ctcatttcaa
     2281 gttttccaac tcttatatga gctaaacaaa aatctacatc tgtataagcc tctttagggc
     2341 atgttgttgc taaatactct atattagggt ccttttcctt tattagtatt tcacaagctt
     2401 tagctacctt attttgtctt tcttcatcat tatcatataa tttgattgct cttaatggta
     2461 acctatctaa gttatctagc aacattaata tgatttctgg ggtatatgta cttcctcctc
     2521 cagctattac tactgaaaat ttttccataa taatatctcc ttttctattt aaaaatttac
     2581 tgtatttata aataaattta taaatactag tactttaaag taaaagggag ttcatttatt
     2641 aaatctattg atttaataaa ctatagccaa acttcaagca ctatttacaa gaaatttaat
     2701 tcttattaat tcttgggtcc atcatctcaa atatatctac aagttcagat actaaagaat
     2761 gagcaaataa tgcatttgaa taatggtctt cagagtgcac aactaaaaaa tcagtcccac
     2821 tatcttcact attcatagca tttgatagta attctaaatg atgctttgat gctgtttgta
     2881 ttttctctga ggctgaattt aaaaggtgtt ttgctttatt gtagtcacct ttttttattt
     2941 ccttaagaga catcattaca tcacttttag catctcctgc ttctgatatt atactaaata
     3001 ctttttcttc catttcaggt accatatcct tctccttcta atgacttgtt aaaaatcaaa
     3061 gctatcatca tctaatgttg tagctaattc tgcattttta tttaattctt ccttctccat
     3121 tatcttaaag aatggataat atatcaaggt cataagtatt atacttgcaa atactaatac
     3181 gattgccttc caatctaatg tcatcaaaaa tgctgcaacc cctggaggta aaaagcctgg
     3241 tggttctgca ataggtaatc ctactaatcc tacttttaat gccataaaat taagtgctcc
     3301 aaccaataca ggtccaaata caaatggtac aaacatatat gggttcataa ctattggtgt
     3361 tccaaatatt accggctcat ttattccaaa tagtgatggt aaaatcacaa cttttcccat
     3421 actattaaaa ctcttacact tacttcttga cataaggtac actaatccca aagttatccc
     3481 tgaaccagtt acttgcaaga aaccatagta aaaacctatt gtaaacacat attttgggtc
     3541 tcctcctgct gctattaacg ttgcattttc tgccatatat gtagctgcta ttggcatcca
     3601 tatacttgaa gttattgctg ctccatgaag cccaaagaac cataataatt gagttataaa
     3661 tagtattata gttactgcaa aaacattatc tacactgctt atagctggtg ctaagaaatt
     3721 cattataata gctggtaatg gttgacctga gacattttta gttattgctg ctattgctgt
     3781 aaataacatt atacatacta tcattggagt taagtttcta aaacttttat ttactacact
     3841 aggtacttct tttggaaatc taattacgag atttttatca ttcataaact tagtaatatc
     3901 aactactaaa agtgctacta atatacttac aaataaacct tgtgcatcta aataacttgt
     3961 atctaataca ccatctacta cttgtccaca tacaacaagt tgtgcaacaa ctgctgtcat
     4021 acctatttct acatgattaa gattatattt tttagctagt gcctgagcta taaaaaatgc
     4081 tgaatatata gataataaac tcatagtaaa tttattaggg ataagtaatg tatccatatt
     4141 ttcagataat aatgtttttg cacttccttc aggtaacata ttgtttaatg ccataaataa
     4201 catacttaaa gaaccaacaa ttattattgg ggtaatgctt atcatacctt cttttatagc
     4261 ttgaagatgc ctttgttttt caatcttttg tgctattgga agaagaaatc ttcttaaatt
     4321 attttgaagt gcttccattt ttattccccc caattatcta tttttatata attcgattgc
     4381 cgacttcata acagaaggag catccatacg accataatgc ttagtatcta ttaaaccaac
     4441 tgctattttg taattcttac aagcttcaac tacatcatct tccttaaacc taatttgtgg
     4501 tgctacaagt actacatcgg tatcattagc ttcaatgtat gattttaaat tttcaaatga
     4561 tgttgcctct atatttatag ttttagtttt taacttttca tgctcactac aaaaactttt
     4621 catcttttcc acaagtacac ctgttgaagc tccatttgca catactaata aaatattcat
     4681 aattaccctc ctacaatatc taacctcttt tattttctaa ttttttatca gccaattctt
     4741 actctaattc ctatcagttt ttactttaat tcttattatt ttattttaat tcttattaat
     4801 atttactcta attcttagta ctgccaatga ttaaacttga aagccgtatc tataacttct
     4861 tcttgcttat atttttctaa tatttttaag tatctttctt tataatcatt atctacttct
     4921 ttttctggta ttactatact agcttcattt aaaaagtgga atgaatccct tcccatttct
     4981 aatcctctat atgtatgctt gtcaagtgcc acgtccataa cttctggaac tttacccatt
     5041 gcaaatcctt ttataattat attttttaat aaatcacttg atctatcttt ctcacatgca
     5101 caaagatatc ttattgcatg tatgaaaaat aaaggtctgt ctccatctgc atatgaatat
     5161 tcctttctta cttgatttaa attatttatg attattacag ctgatgggtc ccccattcct
     5221 atatcttcaa ctgatattgt aagtatcctt ctccatagct tatcttctaa ttctggtgat
     5281 gatatatata gttcataagc aaattcacaa gcttcttcca ccatatttct tcttatagct
     5341 ttttgaaatg acgatattat ttcatctgca ctaaatccgt ttcttgtagt cattcttgcc
     5401 catgggtcat ttgccttttt atctcttaat attttatctt gcatattatt tactcctttt
     5461 tatttaaatt ttaattattt atattactca tttaataata taagcaagta ttatgccagt
     5521 ttttttagac actttaaaac actgttattt caatcatttt tatcatcaaa acttagatac
     5581 tttgtctaag tatatttata tgtgtctaat attaatatta ttttagacta aaaaaaatta
     5641 ccaaataagt ttttatactc attggtaatc tttttcgatt atttctgcat attctaatat
     5701 taataatatt tgatagcact ctttttcatt aatttctata ttatattttt tatctaattg
     5761 agatactata tcttttattt tgcttaggct cttgtgataa tcaagtgttt tttctttatc
     5821 aagcttttca atttctctag tatttccaat caatccatct accatgaaag ctaaatgcat
     5881 cataagtcca attactttat cgtatttctt ttctatatta aatactaaaa acaattcatc
     5941 agacacatat tttatatctt taaatatcct attataatta gagcttttta aggatgtctt
     6001 taaaccttca actacatcat cataacctat acttatcttt ataatatttt ttaatttatt
     6061 gatattttca tccttatcta tatctttttt gctaatatag tcaattcctc taatattaat
     6121 tctttgttca ttgattactg caacaatatt actactttct ttaagtttcg taatatttaa
     6181 acttaaatct ttttctgttt tatatgggac agatataata tctattccat ctaagtacct
     6241 actcatcttt tcttctaata tttttcttgt tttattatct acaccttctg aaacatgtga
     6301 tgctataata attgttttag acagattttc tttattttga atttcattag ttccaaaata
     6361 gtactttctc gtgttagtta tagagttgta tacgtcattt aaaggtaatc ccatattggc
     6421 cttctgaact gcttctaaca cagttaatgt tgtcaccata ggaattactc ttactccaca
     6481 ctttttttct ttctttataa tttcatcaaa agagtttaat gaacccatat ctgtaaatag
     6541 taatacatct ttattgtcta tattattaat taaattttta aaatctacaa taacttcatc
     6601 atagctttta tctaacttca tatcgaaaga aatagcactg cactttccaa taatatcacc
     6661 aactgctttt accatactac ttgctgtagt ttctccatgc atagcaacta taatattaat
     6721 gttattcact attttatttt gttcatcttg tttagcttgt actatgaata atgtaatata
     6781 tgctacagtt tctatagata aaaataaatc aaattcattt tcaattattc caactatttt
     6841 tagtgctgtt ttaaattcac ttctgttatt tcttctaatt tgattcaaat ctatgttgtt
     6901 tgtagagcta cttttatcca atacgctttt tatataaatt gccattgact taaatattga
     6961 ttcacttatg tctatttctt catcttcttt aataatttgt cttattgatt cacacatgct
     7021 tttaaactta acattattaa atagagaagc atctgctata ttcatttcta cagatttatt
     7081 aagattagta tttactgcat taataatatt tatatcattg tagttaataa cttcttcttc
     7141 tcctaatgac gtaaatacaa aatattctat tccatgtgtt ataacttcat ctgctttaat
     7201 agacttctct ctataatcat cactcacttc atttatagat ttatcaaaat attttttctc
     7261 tatataaact ggttctttat tatctctttt atatcttagg tatccatttg caacagctaa
     7321 ttttatatca ttttgaagtt gacctacatt atttttacaa tcataaagta atagtgattt
     7381 aattatttct ttatgtactt ttattgaaat agaaatactt tttgattctt ccttgaaaaa
     7441 atttttgact aattccattc tttcaatcat tgtcctatct ctcagtgatg gtatttctat
     7501 tttcattggt attcttctat tgaatgtatc taatagtgtt gatgtcggat tttctgtagt
     7561 agcacatatt attaatacct tagactctct ttctttcgtt gcatctccta gtcttttaaa
     7621 tgttccctta tctataaaag taaacaacat ttcttgacct tctgctggta gtctatgaat
     7681 ttcatctaaa aatagtatcc ctccatttgc aatttccaaa agaccttttc tggaagattc
     7741 tgcacctgta taagctcctt gttctacccc aaaaatatgt cccataagta attgtgcatt
     7801 attagcataa tcagcacaat taaatgttac aaaaggagct ttattttttc taattccact
     7861 atctatagaa taatcgtaca tatatctagc aatcatactc tttcctactc ctgtttctcc
     7921 atagataata gtatgaagtc catttgtagg atagagcatt gcgaccttgc atttttctat
     7981 tataggtctt aaactatgtt ctttacctat tagtgtttca aaactatctt tttttatttt
     8041 gttttctact tttgttcctt tttttatgcc aagcaactca ctttgtgtat caagcaattc
     8101 attcttgtta aggttaagat ttgcagggat ataatacaca ggtcttcctt ttatcttcgt
     8161 aaccataccc tcattgaata attggtttaa atatgtactt atattgtttc ttgcaattcc
     8221 tgtcttttct gataattggt tagctgtaat ccctttttca ttttccctat atgatgattc
     8281 taaaactttc attactttat cttttttata tatcattaca agctccccta cattatagta
     8341 ttttattaac taaaacccct atacacactt tattttttaa taaactctta ttctttaact
     8401 aaggttataa tttatcaaac tttccttact ttaaattata gaaaattatt gtagaaaaaa
     8461 taagattatt gctcattttt tctacattac tatttttaat tttatagtac tttcttaaca
     8521 acatacttta cttgttttta attttattat atttgaattt attatatttg aaaagtagaa
     8581 ataaaaaacc ctattttgga gtagttttaa aatttctcca aaaatagggt tttagtttgt
     8641 atttcaaata aatacctgtt caagtatttt tatactaaac aacttttaaa ttttgttgat
     8701 atttatttat ttctgattta gaagaatata caaaatgtcc atctgaaaca tcaatccaac
     8761 ttaatgactt ttcatttgaa caatcatatc cattagaatt atactctatt ctatttcttg
     8821 acttagcata tctagggtca ggaacaggaa ttgcagaaag tagtgatttt gtatatggat
     8881 gaataggatt attatacaat tcatttgaat ctgccaattc tactaatttt ccattataca
     8941 taactcctat acggtcacta atatgtctca ccatagataa atcatgagct ataaataaac
     9001 aagttaaatt cttttcttgt tgcaattttt taaataaatt tactatttgt gcttgtatag
     9061 acacatccaa agaagctata ggctcatcag ctattataaa ttctgggtct actgaaagag
     9121 ctcgtgctat gccaatacgc tgtctttgtc cacctgaaaa atcagatgga tacttatttg
     9181 cataacttct atcaagtcct actagcttta gcatctcata tactttattc attctatctg
     9241 ttttattttt acagatacct tgaattttta gtggctctga aattatatct gcaattgtca
     9301 tacgtggatt taatgatgaa gtagaatctt gaaaaataat ctgcatactt ttgtttacat
     9361 ctttttttat aaatttgtac gtttttttat ctgatatgca atttcctttg taaattactt
     9421 ctccaccagt agattcatgt agtcttatta tagttttacc agttgtagat tttcctgaac
     9481 ccgactctcc taccaatcca aatatctcac ccttatatat atcaaaactt atatcatcaa
     9541 ctgcttttac agtagtactt ttgtccaaat gaaaatattg ctttaagttt tttatttcca
     9601 gtattttttc tctattcatt agaaatcacc cttccacaat ttactctaac tggtacatct
     9661 acttcaggtg catctgggtg caataaccaa gttgctgcac tatgtgtatc attaatttta
     9721 aacattggtg gctctttttc ataatctatc ttaagtgcat ttttattcct tattgcaaaa
     9781 gcatctcctt ttggtgggtt caataaattt ggaggcattc caggtatatt atataaatag
     9841 tcatcctgcg aatctaaagt aggtaatgaa cccaacaacc cccaagtata tggatgtcta
     9901 gggtcataaa atatatcctc tacagttcct atttctacaa tctttcctgc atacataact
     9961 gctattctat cagctatagt tgcaactact cctaaatcat gagttataaa tattattgac
    10021 aaacctattt tatgttgtaa atcctttatt aaatcaatta tttgagcttg tatagttaca
    10081 tctaaagcag ttgtaggttc atcagcaatc aatacatctg gattacaagc aagtgctata
    10141 gctattacta tacgttgtct cattccacct gaaaaatgat gtggaaattg tttaaatctt
    10201 ttctcaggat tatcaattcc aaccaattca ataagctcta tggctctctt ttttgcctca
    10261 cttttactta taccttgatg aatgattaca gcttctgcaa tttgctttcc tattgatata
    10321 gttggattga gagatgtcat agggtcttga aatatcattg atatattctt tcctcttata
    10381 tcttccatct ctttttcaga cttcttaacc aaatcacttg atttaagtaa aacctctcca
    10441 tttttaatat atccattgta tgggagtatc ctcatcaaac tcttacacag tacagatttc
    10501 ccacaaccag attctccaac tatagccaat gtctcacctt tttttagatt aaaagataca
    10561 tttcttacag cctgaacttc tccttcttct actttaaaac ttacagatag attattaacc
    10621 tccaataaat gttccaaaaa ccatcaaccc ctatgctatt caaaccttag ggatatattt
    10681 ttatccctaa ggtttctaat taatttcagt attattcaat tgtccagtca gctatgttcc
    10741 agaatatacc tacaccatga tgacctaaaa ctgtatcagg tgttaatcct tttatgtttg
    10801 gttttccaac atatattgca tctatataag caataaatgt ataaggcata tccttagtca
    10861 tttctacttg gaattgttta tataacttta atttttcatc tttatcttct gtttctcttg
    10921 ctttttgaag tattttatct atagtaggat tagagtaagc actgtaattt gcacctttat
    10981 ctgttccaaa tactttatat gtatggtcat ctgggtcaaa tggacttccc caacctatta
    11041 aatgagcatc ttgattagcc caatctgttt cagttacaac aacagcttta gcatcaacac
    11101 caatttcttt taactgttgt gcacatattt tagccatatc aactctaact tggtcactct
    11161 cacctgctgt tatctcgaaa gctaacttag taccttcttt ttcatagatt ccatcagaac
    11221 ctaatttcca acctagcttt tcaatttctt gtttagcttt ttctggatta tattcaaact
    11281 tttctatatc aggattatta tatggtccca tttgtaaagg tgaatatgct ggtacaccat
    11341 gtcctaataa tacactgtca actattgctt ttctatctat tgcatagctt aaagcatttg
    11401 gtaatccttt agctttttta tctttaaaga atttagaatt aaaattgtat aatatacctc
    11461 tatagtcggc tgttttcatt atatttactt taaaattctt ttcgtctttt tcaaagttag
    11521 acatatcttt aggagttatt tgagctaggt ctaattctcc tgattttaat tgcattgcct
    11581 tagccttatc atctggaaca atcttaaaga ctactttatc taatcctggt tcttttacaa
    11641 aataatcact attcttaaca agtgttatac tttgaccttt gtcccatttt tcaagtttaa
    11701 atggtccagt acctattggt ttttgattaa attcatcagt agctatatcc ttaccttcca
    11761 aagcatgttt tggtaatact cctactgtta aataatcaag cattgctgta tttggagctt
    11821 ttaaagtaat ttttatagta ttatcattaa ccacatcaat ttttgttata tcttcataat
    11881 ttgatgctat ctcagaagca ttatctggat tcattatagt ttctattgta aacttaacat
    11941 catttgctgt aaatttttct ccatcatgcc acttaacatc atctcttaaa ttaaaagtat
    12001 atgtatttgt tgcctcatca aatttccagt cctttgctag acaaggaacc accttattat
    12061 tttcatcatg agctgttaat ccattaaata ttaacgaatt tatttctcca tgttcatata
    12121 gtgcaggatt tatacttgta tagtcattac ttccataaac aagcaccttt ccatctttag
    12181 gcgtatctgc cttcttatct ttgtctcctc cactagaaca acctgccatt agactaagta
    12241 tcattactaa acttaaaact tttaacttct ttaatttcat gatgctcccc cttttgcttt
    12301 ttataaaata tttttcaaag ctttgacccc atttatttaa atagatattt caaatacctc
    12361 agtatttgct tatttaatac tcaaatgctg atttataaat tactagattt tttattatta
    12421 ttctttctta tataattacc tatatttgtt atacacacca atgtggtaac caaaaatata
    12481 cccggaatca gtataatcca ccatctattt gaaagtaatg cttcctctga caaagataac
    12541 atacttcccc aagaaacaat ttctattggt aaaccaattc ctaaaaagct aagtgtcgat
    12601 tcagtaccaa tagctgcgcc tatgtttgtt acaaccatga acattattga tgccacaaaa
    12661 tttggaagta aatgttgctt taatacatag aagaatcctc ctcccatgct cttagctgct
    12721 agtatgtatt cgctattttt tatttgtcta acttctgttc tcactatctt agatatattc
    12781 atccaacttg ttatccctat tactatggac atagatactg gatttgaatt tccaagtata
    12841 gcttgtacaa atattataat taatattgaa ggtatactaa gtatgatttc tgtaaacctc
    12901 atcattgcat catctacaag ttcagatgct gaaccactta tactaccata aacaattcct
    12961 ataacagttg atattacagt ggaaaatagt cctataaata aagatatttt tcctccatac
    13021 catatcatag aatatatatc tctacccatt gagtctgttc caaataaaaa agttttattt
    13081 ggagctaaat tagaacttgc taaatccatg tatgttggtt catgtgtcat tataaaagat
    13141 gagaatacac ttccaatgac tatgattgat aaaataataa ttgatatata tggtaattct
    13201 ttaatttttt tagataaggt tttctttgtt tctatatgtt catcttctgc tactaaaata
    13261 taattttctc ctacaatttc aaaatcacta tattgtggct tcattagtat cacctctatc
    13321 atatttcatt ctagggtcta ttttattgtt tataatttga gctaacatat tcccaaatac
    13381 tactaaagcc cctgttatta agcataaaac taataacata ttataatcat gatactttgc
    13441 actttcaaaa caaagtgttc ctaacccagg ataagaaaaa actttttcaa ctacataagt
    13501 tcctcctagt atatgaggta tcgaaatggc cattatacta atatatgatg gcattatatt
    13561 tctcaggcaa tgcttaaaaa caatagttct attatttaaa ccttttgctt tacataaaag
    13621 cacataatct tctcttattt cctctagtaa tttatttctt atcatatatg tgtaatacca
    13681 tagatggctt aatataagta ctgtaagagg taagattaga tgtgatatcc tacttaatat
    13741 actggactct tctcccattg aataagcacc actacttgga agtatactta agtttatact
    13801 aaatataagt atgagtacta aggcaaccca aaatgatgga atacagttgg ttattgttcc
    13861 taatttgcat atgattctat caatgagctt atcttcatgt aatgtacaaa atattcctaa
    13921 caataaggct agcacaaagg taagtatata tcctgaacca cctaatatta tagtgtttat
    13981 atagacatca tttataacag aagtaacatt ttgtttatat ttaaatgaaa ttccaaattc
    14041 cccttttgat gcatttgaaa tccattttat atattgactg tatattggtt catccaaacc
    14101 aagttttttc atagcatttt ctttttcttg tgtactcatt ctttctactc catccccata
    14161 gtaagagatt aatgggtctc ctggagcaag tcttgccatg taaaaaacaa taaaagataa
    14221 taaaaacatt actaaaataa actgcaacaa ctttttcagt atatccttta acaaacccca
    14281 accccccttt gttgcaataa aaaaaaccac ataaaagcaa acgtaaagta acgttaaaac
    14341 cttcgtggtt ccattatcat taaacaaaat taaattttct atatttatct atgagttaat
    14401 tttagcat
//
LOCUS       DAYAPA010000113        14213 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.115, whole genome shotgun sequence.
ACCESSION   DAYAPA010000113 DAYAPA010000000
VERSION     DAYAPA010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14213
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.115"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            307..846
                     /locus_tag="ACGI59_003004"
     CDS             307..846
                     /locus_tag="ACGI59_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007286148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFG9740782.1"
                     /translation="MDIGEKIKRLRTEKQLTQEELANRCELSKGFISQLENNLTSPSI
                     ATLIDILEILGTNLREFFNEIDDERISFTKEDMFETEDEDLKYKLKWLIPNSQKNEME
                     PIIITLYPGGQYKEEKPHEGEEFGYVLAGSIYVHIGEKKNKVKKGESFYFRPKANHYI
                     SNEGKTTAKVIWVSTPPSF"
     gene            865..1908
                     /gene="potA"
                     /locus_tag="ACGI59_003005"
     CDS             865..1908
                     /gene="potA"
                     /locus_tag="ACGI59_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:1902047 - polyamine transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein"
                     /protein_id="HFG9740783.1"
                     /translation="MVENIIELRDISKHYEELTVLDNFNLDIKKNEFLTLLGPSGCGK
                     TTTLKIIAGFEYADDGKVLFEGKEINNLPPYERQVNTVFQKYALFPHMDVYENVAFGL
                     KIKKTPKDVIDAKVKEVLKLVALEGFERRQVESLSGGQQQRIAIARALVNEPKVLLLD
                     EPLGALDLKLRQEMQIELKRIQKKLGITFVFVTHDQEEALTMSDTIVVMYKGKIQQMG
                     TPQDIYNEPKNSFVAKFIGESNIFDGIMIEDYKVNFCNRDFECVDKGFEKNENIEVVI
                     RPEDIKMVKPEEGMLKGKVTSTVFKGVHYEIELNENGRLWILHNTKNAEVGTELGMDI
                     YPEDIHIMRKENN"
     gene            1910..2746
                     /locus_tag="ACGI59_003006"
     CDS             1910..2746
                     /locus_tag="ACGI59_003006"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9740784.1"
                     /translation="MKRKSFLAYPYVVWSAIFVIIPLILVVFFSFTKESGGGYAFTLE
                     NYKEVIDPIYMKVFGRSILLAGGATLICLIVGYPVAYIISKARVSRRGSLILLFILPM
                     WMNFLLRTYAWVAILGKNGLLNTFLGWFGIQPLAILYTNFAILLGMVYNFLPFMVLPI
                     YTALSKMDNDLINAAHDLGANNMTVFRKIIFPLSLPGVMSGITMVFMPAVTTFAISRL
                     LGGGKIMLVGDLIEQQFTVVGDWNFGSAISIFMMIVILISMSIMSKFGDESDKEGGGL
                     LF"
     gene            2851..3525
                     /locus_tag="ACGI59_003007"
     CDS             2851..3525
                     /locus_tag="ACGI59_003007"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9740785.1"
                     /translation="MARWNGFTWKWYGQLLQNESIMSALYYTIVIAILASVISTIVGT
                     ISAIGIHKMRGKSKKLILNVNYLPILNTEIVTAVALMSLFVFVKMEFGFTTMLLAHIM
                     FCLPYVILSVLPKMKQLPDNIEDAAMDLGATPIYALRKVILPQIKPGIVSGFLIAFTM
                     SIDDFIISFFNAGNGVSNLSIEIYGMARRGIKPEINALSTIMFAVVLGLLLLANKKES
                     IVRGIK"
     gene            3525..4577
                     /locus_tag="ACGI59_003008"
     CDS             3525..4577
                     /locus_tag="ACGI59_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HFG9740786.1"
                     /translation="MIKFKKAISLIAVGMMTVTLFTGCNKATDSTKVLNVYNVGDYID
                     ESLIDKFEKETGIDVQYSTYDTNEMMYQKVKSGSTHYDLVFPSDYMVEKMKNEGLLEK
                     LDFKNIPNMKYLDKSFLNPVYDKTNEYSVAYMWGTLGILYNKKEVKDPMDSWNILWNP
                     KYKGNIMMFDSVRDTIGITLKKLGYSMNSVNPKEINEAKNLLMKQKDLVLAYVNDEGK
                     DRLLGGEVAMGMLYSGDAVTLMEQNPDLDYAIPKEGTNKWVDAMCVPTTAQNKKEAEL
                     FINFLLDPENAKVNAEYIGYSTPNTGALKLLDPEITQNPVAYPSKEVLDKCETFEDIG
                     ENIKLYDKAWIELKSK"
     gene            4807..5655
                     /locus_tag="ACGI59_003009"
     CDS             4807..5655
                     /locus_tag="ACGI59_003009"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFG9740787.1"
                     /translation="MNRINKKIDLLILALLVFIFLIVGIVILSIRTENKLNVFIMLAI
                     IFFIIMLTYLSNSVVGLITSSIIIFMYTSYILYNNITHNMDVEFISYMWIIATPVSSI
                     IMGNLNKSINELQNTNKKLSEQYKELVTIDSETGLRNLKIFYNDVNMEISKSIRHNTD
                     FSLMIVKLPYYGNLQTIFGENKTNKIVKYIGSNIIECTRNEDIIYSLQKDMIGILMPN
                     TSLEGSKVVKDRIKKRIKELNLDLNNRGKYVNIDVKIAFLQYKSSFGDSINFKNIVEE
                     ELQYDV"
     gene            5645..7309
                     /locus_tag="ACGI59_003010"
     CDS             5645..7309
                     /locus_tag="ACGI59_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2334 domain-containing protein"
                     /protein_id="HFG9740788.1"
                     /translation="MMFKIFNKSLLKLVVVLSIVLTISNFSLSSYASEITSSTDKVLI
                     IYDSKKETAYNRDILNIMRTLLGRFSSDIELLKLSNYDGEINKNYYSHIFILGINENS
                     YNNDKNTKNLISSLNLYKGTICWLGYGIENLLEHKKYNLDYVGKTNNIVSVNYRGKSY
                     NLDEHYVFNIVESKDTSNKVIGSINDTLNKYPYIINDKNLFYVSKLDLDGVLFYIFCD
                     SLNDIFNIKTFDKGRIFVRIEDVHAFREPKNLVEIADYLSSKNIPFTIALIPAYVNPK
                     NHKVITLSESPEIVKAIKYMQDKGGTVILHGYTHQYKKEEVSGEGYEFWDGKKDEPLK
                     ENMKIFVKDRVLNGLRVCIENGIYPLAFEAPHYAMESEGYKELKKYFSTYIGQHQNND
                     KKFSTNTYPYIIRDTEEFNIFIPENLGYIDPEDKFTFQNIKENLDKLSIVRGFSGGFF
                     FHSYLNIEYLKNTIEYLEKQNIEFMNLRDFNNWVKVDEIQIRNNGDEIIVNYDKDLDE
                     LTKSDTRFKSISNISKVLIFIVSISVLIFVIIFIYFKRIDKKKFLK"
     gene            7358..8614
                     /locus_tag="ACGI59_003011"
     CDS             7358..8614
                     /locus_tag="ACGI59_003011"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFG9740789.1"
                     /translation="MRDLNVSEYLFVFSLFSIWSLLLINIILAMGGYIFYFKNFDKEI
                     KEIDEYPMISILVPAHNEAKVIGRTVESLLLLNYPKSKMELIVINDNSSDNSKEILEN
                     IKDRYNNYNFTIINTDSLTGGKGKSNALNIGYTISKGDFIAVYDADNTPDKNALRYLV
                     QTIVMNDELGAVIGKFRTRNKNKNLLTKFINIETLSFQWMSQAGRWQLFNLCTIPGTN
                     FILRRSIIEEIGGWDSKAIAEDTEISFRIYKLGYKIKLVPQSITWEQEPETVKVWIKQ
                     RTRWAKGNIYVLMKYIKNIFKQGRNKIVFDIAYFFSVYFLFLTSVIISDILFVLSISK
                     LVEISIPINFFLIWILSYLLFIIEVSISLTIEKGEATIENIFIVAIMYFTYSQLWLFV
                     AIKGMIEYLKDIIFKREVKWYKTERF"
     gene            8637..10718
                     /locus_tag="ACGI59_003012"
     CDS             8637..10718
                     /locus_tag="ACGI59_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HFG9740790.1"
                     /translation="MKKFIISIISLVLFFSNISLIYKVNADETKVKNYKFERDITIDG
                     VIGSNSTFFEVNKNWDIEEVLLHLNFSKSQILNGDVSSLTVLINNVPIKSIKLNAKTN
                     YKNTLEVLVPKDYIIQGYNEIKIKTYKTISDKICQDDSNTGNWMVIHKESYISIRYKQ
                     KKVENSINEYPYPYAEIENNHKLDTTIVVPDNMTRGETTAVFNLASAFGKITKNDDLK
                     LDVKLYSEMKNWSDDNIIYIGKPENTAEEILDILSIKEQTLLSSNCIIKQVDSPYNKN
                     KKMMVVIGSNEDDLIKASNLLIENRLSNQVLSSSVLVNKETNIKINREQKLNLGHLTL
                     KDLGYSDFLLEGAFNQQALFDVKIPTGKVLDDGSKIILNLRYSDNLDFEKSLVTVSIN
                     DVIVGSKKLDRSHSNNDKLELKIPKDIDNKNYYQVKLTFNLSIKNSNCVTRESNNPWA
                     YVSNNSYLALSTKENETLSFENYPYPFVRDDEFNDLTVIMPDYSGSQAMTWMFRLGVT
                     LGANINSHNGNINVIRGKEFSDKYKDTNIVVFGVPHNNSVIKMLNNNLNIKFDKNYSN
                     FISNDKISFIDDYGKNISTIQLIKSPYNNQKNIMVISSMNEKNLYLGMDYLLNKSKVN
                     DLKGDTLIIDEYGEVEDLAYNLKSKKEVKDSSWNMSINKTTKVFLMISFITIIIVMIL
                     SMLYIKKYKRR"
     gene            10755..11840
                     /locus_tag="ACGI59_003013"
     CDS             10755..11840
                     /locus_tag="ACGI59_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFG9740791.1"
                     /translation="MRKYMNEFKLKRSILISLIILIFIFIVYFVSIELKDRSLVNNQY
                     KGKIKSANLSVDYEINQVMKDIDKLGLNTVNVPIIINVESINSDIMSIDNNSKEKAIK
                     LIKKLNKKGISTILEAYPWIENGKLYETDWNPINKKRFFYTWQNAILNELIIDVANPL
                     DVNVLNIGSNFVHLEEYQQNWGEIIDFVQSKFDGLVTYRTNWWYTKKDDLSSKLFYEQ
                     KLNNNFFDKLDFISIAAYFELSNKPVNTVDELISALHSSTVNNRGQNIKQEIYNLYKA
                     HRKPIFFGELGFSNRESASSQPWNHTPSKAVNGEEQARCFEAYKKVFENEDWINGFSV
                     FCVGKIDDEKNFYPSKESIKVIKSWYE"
     gene            11894..13018
                     /gene="wecB"
                     /locus_tag="ACGI59_003014"
     CDS             11894..13018
                     /gene="wecB"
                     /locus_tag="ACGI59_003014"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HFG9740792.1"
                     /translation="MNDIKVMTVFGTRPEAIKVAPLIKELEKRENIKSIVCVTAQHRE
                     MLDQVIETFNINVDYDLDIMEKGQSLNDITCKILNKLPLILNKENPNIILVHGDTTTT
                     LATSLTAFYNKTLVGHIEAGLRTYDKYSPFPEELNRQLTGIIADMHFAPTNLARKNLI
                     SEGKPNNNIFVTGNTAIDALKMTIKENYNHPIIDEIGNDRMILLTSHRRENLGKPMKN
                     IFRAIKRIVDDFEDVQIVYPIHLNPKIRTIADEIFGKFPEKIHIIEPLDVADFHNFLN
                     KSYMIMTDSGGIQEEAPSLGKPVLVLRDKTERTEGIEAKTLKLVGTNEDRIYNSVSDL
                     LINKDNYVQMSKASNPYGDGNASKYIVDIIIKKFNCKYLN"
     gene            13096..14070
                     /locus_tag="ACGI59_003015"
     CDS             13096..14070
                     /locus_tag="ACGI59_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFG9740793.1"
                     /translation="MARKLIKNLGKSKSVKRVKLLFKKIFITVFIVASIVAIFNITKY
                     FEELYKVRDLKSTKIEYYMDVADEAGDGKVQLSWKALLAIDMVIHDEDLSNIKKKDTL
                     DIGEKFIVEDKNGKGEKVYKVKKFNKVLSELKFDSSQKSRARKYMKDLEYTYLGNKQL
                     DSSDEKIKFIKKLEDSAIREYIDYGILPSITIGQAILESGWGNSKLTKQSNNLFGIKA
                     DKAWKGKSVEISTSEHYNEKIVASFRSYNSLQDSVKDHSLFLINNKRYRKHGLFEAKD
                     YISQAQALENAGYSTAEDKKGNRIYAELLIDVIRSYNLQLIDNKVETK"
BASE COUNT         5806 a         1375 c         2396 g         4636 t
ORIGIN      
        1 attttttatt tgttcaatat ttttttagta ctagaagtct tattctctat ttaatgaatg
       61 aattaattta taattagaga tactaaactt tatagatatt aatataaaca cctttaaact
      121 gtgtattttc caaagccttt aaaatgcttg tatttattct aaataaaaaa ataaaaaaat
      181 gtcaaaaaaa ctcttgtatt atatccaaat tttatatata attacctatg ttgtttagaa
      241 attttaaact caatgtttag taaacaaaat gtttagtaaa ataaaacaga aactaggagg
      301 tgcaacatgg atataggtga aaagataaaa agattaagaa cagaaaaaca actgacccag
      361 gaagagttag ctaatagatg tgaattgtca aagggattta tttctcaatt agaaaataac
      421 ttaacatcac catccatagc tacccttata gatatacttg aaatactagg tacaaattta
      481 agggaatttt ttaatgaaat agatgatgaa agaatcagtt ttacaaaaga agatatgttt
      541 gaaacagaag atgaagattt aaaatataaa ttaaagtggt taattccaaa ttcacaaaaa
      601 aatgaaatgg agccaataat aataacattg tatccaggag gacagtataa agaagaaaag
      661 ccacatgaag gtgaagaatt tggatatgta ttagcaggtt caatatatgt ccatataggc
      721 gaaaagaaaa ataaagtaaa aaaaggagag agtttttact ttagaccgaa agcaaatcac
      781 tatatatcaa acgagggcaa aactacagca aaagtgattt gggtaagtac gccaccatca
      841 ttttagaata gaggtgttta ataattggta gaaaatataa tagaattaag ggatatatct
      901 aaacattatg aagaacttac tgttttagat aatttcaatt tagatataaa gaaaaatgag
      961 tttctaacat tacttggacc aagtggatgt ggaaaaacta caacattaaa aataatagca
     1021 ggatttgagt atgctgatga tggtaaagtt ttatttgaag gtaaggagat aaataatttg
     1081 ccaccatatg aaagacaagt aaacacagta tttcaaaaat atgctttatt tccacacatg
     1141 gatgtttatg aaaatgtagc ttttggatta aaaattaaaa agactccaaa agatgtaata
     1201 gatgctaaag taaaagaggt tttgaaactt gttgcactag aaggttttga aagacgacaa
     1261 gttgaatcat tgagtggagg acaacaacaa agaattgcaa tagcaagagc tctagttaat
     1321 gaaccaaagg tattattact agatgaacca cttggagcac ttgatttaaa gttaagacaa
     1381 gagatgcaaa tagaattaaa gagaatccaa aaaaaattag gaataacatt tgtttttgta
     1441 actcatgacc aagaagaggc tcttactatg tcagatacta ttgttgtaat gtataagggc
     1501 aaaattcaac aaatgggaac accacaagat atatacaatg aacctaaaaa ttcatttgta
     1561 gctaagttta taggtgaaag taacatattt gatggaatta tgatagaaga ttataaagtt
     1621 aatttctgta atagagattt tgagtgtgtt gataaaggat tcgaaaaaaa cgaaaatata
     1681 gaagtagtaa taagaccaga agatataaaa atggtaaagc cagaagaagg aatgttaaag
     1741 gggaaagtta catctacagt gtttaaagga gtgcattacg agatagaatt gaatgaaaat
     1801 ggaagattgt ggatacttca taatactaaa aatgctgagg ttgggactga actaggcatg
     1861 gatatatatc cagaagatat ccatataatg agaaaagaga ataactaaga tgaaaagaaa
     1921 atctttttta gcatatccat atgtagtttg gagtgcgata tttgtaataa ttcctttaat
     1981 attagtagta ttttttagtt ttacaaaaga atcaggtgga ggttatgcct ttacacttga
     2041 gaattataaa gaagtaattg accctatata tatgaaagtt tttgggcgtt ctattctttt
     2101 agcaggagga gctacattaa tatgccttat agttggttat ccagtggcat atataatttc
     2161 aaaggctcgt gtaagtagaa gaggttcatt aatattattg tttattcttc ctatgtggat
     2221 gaactttctt ttaagaacat atgcatgggt tgctatatta ggaaaaaatg gacttttaaa
     2281 tacattttta ggttggtttg gaatacaacc attagcaatt ttgtatacta attttgccat
     2341 acttttaggt atggtgtata actttttacc atttatggtt cttccaatat atacagcact
     2401 atcaaagatg gataatgatt tgataaacgc tgctcatgac ttgggtgcaa ataatatgac
     2461 agtttttaga aaaattatat ttcctttgag tttgccaggt gtaatgtctg gaataactat
     2521 ggtatttatg ccagcagtca ctacttttgc aatatcaaga ctgttaggtg gaggtaaaat
     2581 aatgctggtt ggagatttga ttgaacaaca gtttacagtt gttggagatt ggaactttgg
     2641 ttcagctata tcgatattta tgatgatagt aatattaata tctatgtcta taatgtcaaa
     2701 atttggagat gaatctgaca aagaaggcgg tgggttacta ttttaaaatt aaagaaaata
     2761 acttctaata tatatttagc attagtattc ttatttttgt atgcacccat atttgctttg
     2821 gtactatttt ctttcaatga ttcaaaatca atggcaagat ggaatggatt tacatggaag
     2881 tggtatggtc aattacttca aaatgagagt ataatgagtg ctttatatta tactattgta
     2941 atagctatat tagcgtctgt aatatctaca atagtaggaa ctataagtgc tataggaata
     3001 cataagatga gaggtaagag taagaaatta atactaaatg taaactatct tcctatttta
     3061 aatacagaga tagttacagc agtagctctt atgagtctgt ttgtatttgt aaaaatggaa
     3121 tttggattta caacaatgct attggcacat ataatgtttt gtttgccata cgttatactt
     3181 tcagttttgc caaaaatgaa gcaattacct gataatatag aagatgcagc tatggacttg
     3241 ggagcaacac ctatatacgc tcttagaaaa gtaattttac cacaaataaa accaggtata
     3301 gtttctggat ttttaattgc atttactatg tcaatagatg actttataat aagtttcttt
     3361 aatgctggaa atggtgtaag caatctttct atagaaatct atggaatggc tagaagaggt
     3421 ataaaacctg aaataaatgc attatcaaca ataatgtttg cagtggttct aggtttactt
     3481 ttactagcta ataaaaaaga atctatagta agggggatta aataatgatt aagtttaaaa
     3541 aagcaatatc ccttatagct gtaggtatga tgacagttac tttatttaca ggatgtaata
     3601 aagcaactga ttctacaaaa gttttaaatg tatataatgt tggagattat atagatgagt
     3661 ctcttataga taagtttgaa aaagaaacag gaatagatgt tcaatattca acttatgata
     3721 ctaatgaaat gatgtatcaa aaagtaaaga gtggtagtac acactatgat ttagtatttc
     3781 cttcagacta tatggtagag aaaatgaaaa atgaaggtct tttggaaaaa ttagatttta
     3841 agaatatacc taatatgaaa tatttagata agagtttttt aaatcctgta tacgacaaaa
     3901 ctaatgaata tagtgttgca tatatgtggg gaactcttgg aatactgtac aataagaaag
     3961 aagttaaaga ccctatggat agctggaata tactttggaa tccaaagtat aagggaaata
     4021 taatgatgtt tgactctgtt agagatacta tagggataac tcttaaaaaa ttaggatata
     4081 gcatgaatag tgtaaatcca aaagaaataa atgaagctaa aaatctttta atgaaacaaa
     4141 aggatttagt attggcttat gtaaatgatg aaggtaagga tagattgctt ggtggagaag
     4201 tggcaatggg tatgctgtat tctggagatg cagttacact aatggagcaa aatcctgacc
     4261 ttgattatgc tatacctaaa gagggaacta ataaatgggt agatgctatg tgtgtaccta
     4321 caacagcaca aaataagaaa gaagctgagc tgtttataaa ctttttatta gacccagaga
     4381 atgcaaaagt aaatgctgag tacattgggt attcaacacc taatacagga gcactaaaat
     4441 tattagaccc agaaataact cagaatcctg ttgcatatcc atctaaagaa gtgcttgata
     4501 aatgtgaaac ttttgaagat attggtgaaa atataaaatt atatgacaag gcttggatag
     4561 agttaaaatc aaaatagggt cttgttggtt taagtaaatt tacttaataa atacttaata
     4621 aaactgttct tttttagaaa atttattttc taattattga acagttttat tttttgttta
     4681 agttatattc atatgcaatt taaatagttt attaataaat gaaaacttat ttgtataatg
     4741 actttaataa taattagaaa aaatgggcaa cttaatagaa taattggagg tgtaaattta
     4801 taaaaaatga ataggataaa taaaaaaata gatttattaa tattggcttt gctcgttttt
     4861 atatttttaa ttgttggaat agtaatacta tcgattagga cagaaaataa attgaatgtg
     4921 ttcataatgt tagcaataat tttttttata ataatgttaa cttatttatc aaattcagta
     4981 gtggggttga taacaagttc aataattata tttatgtata catcctatat tttatataac
     5041 aatattactc acaatatgga tgttgaattt atatcatata tgtggataat agcaacacca
     5101 gtatcttcaa ttataatggg taatctgaac aaaagcataa atgaattgca aaatacaaat
     5161 aaaaaactat ctgaacagta taaggaatta gtgactattg atagtgaaac tggacttaga
     5221 aatttaaaga ttttttataa tgatgtaaat atggagataa gtaaatctat tagacataat
     5281 actgattttt ctcttatgat agtaaaatta ccatactacg gaaacctaca gactatattt
     5341 ggtgaaaaca aaactaataa aatagtaaaa tatattggtt caaatattat agaatgtaca
     5401 agaaatgaag atataattta tagtttacaa aaagatatga tagggatact aatgccaaat
     5461 acatctttgg aaggttcaaa agttgttaaa gacagaataa agaaaaggat aaaagaatta
     5521 aatttagatt taaacaatag gggaaaatat gtaaatatag atgtcaaaat tgcattttta
     5581 caatataaga gtagttttgg agatagtata aattttaaga atatagttga agaggaactt
     5641 caatatgatg tttaaaatat ttaataaaag tctattaaaa ttagtagttg tattatcaat
     5701 agtactgaca atatcaaatt ttagcttatc ttcatatgca tcagaaatta cttccagcac
     5761 agataaggta ttaatcatat atgattctaa gaaagaaaca gcttataata gagatatttt
     5821 aaatattatg aggactctat tgggaagatt tagttcagat atagagctat taaaattgag
     5881 taactatgat ggtgagataa ataaaaatta ttacagtcat atatttattt taggaatcaa
     5941 tgaaaactca tataataatg ataaaaacac taaaaacttg attagttcct taaatttata
     6001 taaaggaact atatgttggt taggatatgg tatagaaaat cttttggaac ataaaaaata
     6061 caatttagac tatgtaggaa aaacaaacaa tatagttagt gtaaattata gaggtaaatc
     6121 atataacttg gatgaacatt atgtttttaa cattgtagaa tcaaaagata caagtaataa
     6181 agtaatagga agtattaatg ataccttaaa taagtatcca tatataataa atgataaaaa
     6241 tcttttttat gtatcaaaat tagatttaga tggagtttta ttttatatat tttgcgattc
     6301 acttaatgat atttttaata ttaagacgtt tgataaagga cgtatatttg taagaataga
     6361 agatgtacat gcttttagag aaccaaaaaa tcttgtagag atagcggatt atttaagtag
     6421 taagaatata ccatttacga ttgcattaat accagcatat gtaaatccta aaaatcataa
     6481 ggtaataact ttatcagaaa gccctgaaat tgtcaaagca ataaaatata tgcaagataa
     6541 aggtggaact gtgatattgc atggatatac tcatcaatac aagaaagaag aagtttctgg
     6601 agaagggtat gaattttggg atggtaaaaa agatgaaccc ttaaaagaaa atatgaagat
     6661 atttgtcaaa gatagagttt tgaatggtct tagagtatgt atagagaatg gaatttatcc
     6721 tcttgcattt gaagcgccac attatgcaat ggaatcagag ggatataaag aattaaaaaa
     6781 atatttttct acttatatag ggcaacatca aaataatgat aaaaaattta gcacaaatac
     6841 atatccttat attattaggg atacagagga gtttaatata tttatacccg aaaatttagg
     6901 atatatagac ccagaagata agtttacttt tcaaaatata aaagaaaatt tagataaatt
     6961 atccattgta agaggtttta gtggagggtt cttctttcat tcatatttaa acatagagta
     7021 tctaaaaaac acaatagaat atttagaaaa acagaatata gaatttatga atttaagaga
     7081 ttttaataat tgggtaaaag ttgatgaaat acaaataaga aataatggag atgaaattat
     7141 agtcaactat gataaggact tagatgaact aactaaaagt gatactagat ttaaatccat
     7201 atccaatatt agtaaagttt taatattcat agtatctata agtgttttaa tatttgtgat
     7261 tatatttata tattttaaga gaatagataa aaagaaattt ttaaaataaa ggattttata
     7321 ttagaatcaa tattttaaaa tattttagga gaaatttatg agagatttaa atgtatcaga
     7381 gtatttgttc gtatttagtt tgtttagtat atggtcttta ttgcttataa atattatttt
     7441 agctatgggt ggatatatat tttattttaa aaactttgat aaagaaataa aagaaattga
     7501 tgaatatcct atgatatcaa tacttgttcc tgctcataat gaagctaaag tgataggtag
     7561 aactgtggag tctttgctat tactcaatta tcctaagagt aagatggaac ttattgttat
     7621 aaatgacaat tcttctgata atagtaaaga aattttagaa aatataaagg ataggtacaa
     7681 caattataat ttcactataa taaatacaga tagtttaaca ggtggaaaag gaaagtctaa
     7741 tgcattaaat attggatata cgatttcaaa aggagatttt atagctgttt atgatgcaga
     7801 taacacacca gataaaaatg cacttagata tctagtacaa actatagtaa tgaatgatga
     7861 attaggtgct gttattggga aatttaggac tcgaaataaa aataaaaacc tattaaccaa
     7921 atttataaat atagaaacat taagctttca atggatgtca caagctggta gatggcaact
     7981 ttttaatcta tgtacaatac caggaactaa ctttatattg aggagaagta tcatagaaga
     8041 aattggagga tgggatagta aagcaattgc agaagataca gaaataagtt ttaggatata
     8101 taaattggga tacaagataa agcttgtacc acaatctata acatgggaac aagagccaga
     8161 aactgttaag gtgtggataa agcaacgtac tagatgggca aaagggaata tatatgtatt
     8221 aatgaaatat ataaaaaata tatttaaaca gggaagaaat aagattgtgt ttgatatagc
     8281 ttactttttt tctgtatatt ttctattttt aacatctgta attatatccg atattttatt
     8341 tgttttaagt atatctaaac tagtagaaat ttctattcca attaactttt ttctaatatg
     8401 gatactatct tatcttttat ttattataga agtgagtata agccttacta tagaaaaagg
     8461 tgaagcaaca attgaaaata tattcatagt ggcaataatg tattttacat actctcaact
     8521 atggctgttt gtagctataa aaggaatgat agagtatcta aaagatataa tttttaaaag
     8581 agaggttaaa tggtataaaa cagaaagatt ttgattcatg tacaggagga attaacatga
     8641 aaaaatttat tatatcaatt atatctttag tattgttttt ttcaaatata tcacttatat
     8701 acaaagtaaa tgctgatgag actaaagtta aaaattataa gtttgaaaga gatataacta
     8761 ttgatggtgt tataggaagt aatagtactt tttttgaagt aaataaaaat tgggatattg
     8821 aagaagtttt attgcatctt aattttagta agagccaaat attaaatgga gatgtttcaa
     8881 gtttaactgt attgataaat aatgtaccaa taaaatctat taaattaaat gctaagacaa
     8941 attataaaaa tacattagaa gtattagttc caaaagatta tataattcaa ggatataatg
     9001 aaataaaaat aaaaacatac aaaacaatat ctgataaaat ttgccaagat gattccaata
     9061 ctgggaactg gatggttatt cacaaagaat catatatatc aattagatat aagcagaaaa
     9121 aggtggaaaa ctcaataaat gaatatccgt atccatatgc agaaattgaa aataatcata
     9181 aattagatac aactatagta gtaccagata atatgactag aggagagact acagcagtgt
     9241 ttaatttggc atcagcattt ggaaaaataa caaaaaatga tgatttaaag ttagatgtta
     9301 aattatattc agaaatgaaa aattggtcag atgataatat tatatatatt ggtaaacctg
     9361 aaaatacagc agaagaaatt ttggatattt taagtataaa ggaacaaacc ctattatctt
     9421 caaactgtat aatcaagcaa gtagattcac catacaataa gaataagaag atgatggtgg
     9481 taattggtag taatgaagat gatttaataa aggcatcaaa tttattaata gaaaatagac
     9541 tttctaacca agttttatca tcatcagtac ttgtaaacaa agagactaat ataaaaatta
     9601 atagggaaca aaaattaaat ttaggtcatt taacattaaa agatttagga tattctgact
     9661 ttttgttgga aggagcattt aatcaacaag ctttgtttga tgtcaaaata ccaacgggta
     9721 aggtattaga tgatggttca aagattatct taaatttaag atattctgac aatttagatt
     9781 ttgaaaaatc tttagttaca gtgtctataa atgatgttat tgtgggaagt aaaaaattgg
     9841 ataggtcaca ttctaataat gataagttag aattaaaaat acctaaagat atagataata
     9901 aaaactatta tcaagttaaa ttgacattta atttgagtat aaaaaattcg aattgtgtta
     9961 ctagagagag taataatcct tgggcatatg tatcaaataa ttcttatttg gcgttatcta
    10021 cgaaagaaaa tgaaacctta tcttttgaaa attatccgta tccatttgtt agagatgatg
    10081 aatttaatga tttaactgtg ataatgccag attattcagg gtcacaagct atgacctgga
    10141 tgtttagatt aggtgttact ttaggtgcga atataaattc acataatgga aatataaatg
    10201 taattagagg aaaagaattt agtgacaagt ataaggatac taatattgta gtttttggag
    10261 tgccacataa taattctgtt ataaagatgt taaataataa tttaaatata aagttcgata
    10321 aaaattactc caattttata tcaaatgata agataagctt tattgatgat tatggaaaaa
    10381 atatttctac tattcaatta ataaaatcac catataataa tcaaaaaaat atcatggtaa
    10441 ttagttcaat gaatgaaaaa aacttatatt taggaatgga ttatttgttg aataaaagta
    10501 aagttaatga tttaaaagga gatacattga taattgatga atacggagag gtagaagatt
    10561 tagcttataa tttaaaatct aagaaagaag taaaagattc aagttggaat atgagtataa
    10621 ataagacaac taaagtattt ttaatgattt cttttattac tataattata gtcatgatat
    10681 tatcaatgct atatataaaa aaatataaaa gaagataata tgataaattt attattttaa
    10741 aagaggtata taatttgagg aaatatatga atgaatttaa attaaaaaga agtattttaa
    10801 tctcattaat aatcttaatt tttatattta tagtttactt tgtaagtatt gagttaaaag
    10861 ataggagtct agtaaataat caatataaag gtaagattaa atcggccaat ttatctgtag
    10921 attatgaaat aaatcaagtt atgaaggata tagataaatt gggattgaat acagtaaatg
    10981 tacctataat tataaatgta gagtccatta attctgatat tatgtcaata gataataata
    11041 gtaaagaaaa ggcaattaag ttaataaaaa aattaaataa aaaaggtatt tcaacaatat
    11101 tagaggcata tccatggatt gaaaatggaa aattgtatga gactgactgg aaccctataa
    11161 ataaaaaaag atttttttat acttggcaaa atgccatatt aaatgaactt ataattgatg
    11221 tagcaaatcc actggatgta aatgttttaa atattggttc taattttgta catttggaag
    11281 aatatcaaca aaattggggt gaaataatag attttgtaca atccaaattt gacggtcttg
    11341 taacgtacag aactaattgg tggtatacaa aaaaagatga tttaagtagt aagttgtttt
    11401 atgaacaaaa gctgaacaat aatttttttg ataagttaga ttttatatct atagcagcat
    11461 attttgagtt aagtaataaa cctgttaata cagtggatga attaatttct gccttacact
    11521 catctactgt aaacaataga ggacaaaata taaaacaaga aatatataac ctttataaag
    11581 ctcatagaaa acctatattt tttggagaat taggtttttc aaatagagaa agtgcaagtt
    11641 ctcagccgtg gaatcatact ccttcaaaag ctgtaaatgg cgaagaacaa gcaaggtgtt
    11701 ttgaggcata taagaaagtt tttgaaaatg aagactggat aaatggtttt tctgtgtttt
    11761 gtgtaggtaa aatagatgac gaaaaaaatt tttacccatc aaaagagagt ataaaagtta
    11821 ttaagtcttg gtatgaataa ttttgaaagt gtagaattta tatagattaa atagatgatt
    11881 aggagagata taaatgaatg acatcaaggt tatgactgta tttggaacta gacctgaagc
    11941 tatcaaagta gcacctttaa taaaggaact tgaaaaaaga gagaatataa aaagtattgt
    12001 ttgcgttaca gctcaacata gagaaatgct tgaccaagtt atagaaacct ttaatataaa
    12061 tgtagactat gatttggata taatggaaaa aggacaaagc ctgaatgata taacatgtaa
    12121 gattttaaat aaacttccct tgattttaaa caaggaaaat ccaaatataa tattggtaca
    12181 tggagataca accacaactc tagctacaag tttgactgca ttttataata aaactcttgt
    12241 tggtcatata gaagctgggc ttagaaccta tgataaatat tctccttttc cagaggagct
    12301 aaatagacaa cttacaggta ttattgctga tatgcacttt gctcctacta atttggctag
    12361 aaaaaacttg atttctgaag gaaaacctaa taataatata tttgtaactg gtaatactgc
    12421 tattgatgct ttaaagatga caataaaaga aaactataat catcctataa ttgatgagat
    12481 tggtaacgat aggatgattt tgttaacctc tcacagaaga gaaaatttgg gtaaacctat
    12541 gaaaaatata ttcagagcta taaagcgaat agtagatgac tttgaagatg tacagatagt
    12601 atatcccata catttaaatc ctaaaatacg aactatagct gatgagatat ttggaaaatt
    12661 tcctgaaaaa attcatatta tagagccttt agatgtagct gattttcata actttttaaa
    12721 taaatcttat atgataatga ctgatagtgg aggaatacag gaagaagctc catcattagg
    12781 taagccagtt cttgtgttgc gtgataaaac agaaagaaca gaaggaattg aagcaaagac
    12841 acttaaatta gtaggtacta atgaagatag aatatataat tcagtgagcg atttattaat
    12901 aaataaagat aactatgttc agatgagtaa agcatctaat ccatatggag atggaaatgc
    12961 aagcaaatat atagtggata taataattaa aaaatttaat tgcaaatatt taaattagat
    13021 aaggcctaaa acattaacta attgatataa gatttatgat aatattaagg aatacaattt
    13081 ttaaggggga gtaatatggc tagaaaatta ataaaaaatt tgggtaaaag taaaagtgta
    13141 aagagagtga aacttttatt taagaagatt tttattactg tattcatagt agcaagtata
    13201 gttgctattt ttaatataac gaaatatttt gaggaattat acaaagtaag ggatttaaaa
    13261 agtactaaaa ttgaatacta tatggatgtg gctgatgaag caggagatgg aaaggtccaa
    13321 ttaagttgga aggccttact tgctatagac atggtaatac atgatgaaga tttaagtaat
    13381 ataaaaaaga aagacacatt ggatataggg gaaaagttta tagtagaaga taaaaatggt
    13441 aaaggcgaaa aggtgtataa ggtaaaaaag tttaataagg tattaagcga attgaaattt
    13501 gactcttctc aaaaaagtag agcaagaaaa tacatgaaag atttagaata cacatacctt
    13561 ggaaataaac aattagatag tagtgatgaa aaaattaaat ttataaagaa gttagaagac
    13621 tcagctataa gagaatatat tgattatgga atattgccct ctataacaat tggacaagct
    13681 atattagaat ctggttgggg aaattctaaa cttacaaaac agagtaataa tttatttggt
    13741 ataaaagcag ataaagcatg gaaaggaaaa agtgtagaaa tttcaacttc agagcattat
    13801 aatgaaaaaa ttgtagctag ttttaggtcg tataattcat tacaagattc tgtcaaagac
    13861 cacagtttat ttttaattaa taataaaaga tatagaaaac atgggttgtt tgaggcaaaa
    13921 gattatatta gtcaagctca agcattagaa aatgcaggat atagtacagc agaagataaa
    13981 aaaggaaatc gtatatatgc agagctatta atagatgtta ttaggagtta taatttgcaa
    14041 ttgatagata ataaagttga gacaaaataa ttgttaactt tatatgagga gataacaaga
    14101 taagattttt ttctacaaaa agaatagaaa aattgtaaaa aaaactaaaa aaatgtattg
    14161 acttgttatt taaaaggtga tattattatt actgtactcg aaagagaaca aaa
//
LOCUS       DAYAPA010000114        14202 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.116, whole genome shotgun sequence.
ACCESSION   DAYAPA010000114 DAYAPA010000000
VERSION     DAYAPA010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14202
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.116"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            37..501
                     /gene="bcp"
                     /locus_tag="ACGI59_003016"
     CDS             37..501
                     /gene="bcp"
                     /locus_tag="ACGI59_003016"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HFG9740794.1"
                     /translation="MLSIGTKAPEFTLEDKDGNKVSMSDFKGKKVVVYFYPKDNTPGC
                     TRQACAFRNAYDGFKKEDIQVIGISKDSIKSHQKFAEKHELPFILLSDPDLVAIKAFD
                     VWKEKKMYGKTALGVVRATYIIDENGIIEKVFEKAKPDTNAQEILEYLEKQE"
     gene            511..1254
                     /gene="yaaA"
                     /locus_tag="ACGI59_003017"
     CDS             511..1254
                     /gene="yaaA"
                     /locus_tag="ACGI59_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HFG9740795.1"
                     /translation="MIIMLSPAKNMKNIEVFDRDLSLPCFIDNTKEIVENIKTFGIED
                     FKNKMKINEKLAVLNKNRFESIKFDRLGNPAILTYDGIQYKNIEAENFTRKDEEFANS
                     CIRIISGLYGVVKPYDSIYEYRLEMQTKLQVGEFKNLYEYWGNRIYKELIKEKTAIVN
                     LSSNEYSKSIEKFIKDSDTYITCTFKVNKNGILKVESTQAKKARGMMTKYIVKNRIRD
                     IEELKKFDLEGYKYKENLSNNSEYIFVKE"
     gene            1352..4039
                     /locus_tag="ACGI59_003018"
     CDS             1352..4039
                     /locus_tag="ACGI59_003018"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005388 - P-type calcium transporter
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HFG9740796.1"
                     /translation="MKKRKEWSSKMENHKQMPWHSRATHEILESLDSSEEGLTDTEAE
                     KRLKENGLNELRKKPPRTTLQMLWSQITDAMVMILIGAAILSLIFGEFTEAFVILIIV
                     TVNAVIGIVQEKKAESSLEALKNMNSPTARVMRDGEENVIPASNLVVGDIVFLEDGAM
                     VPADLRLIETSSLKIQEASLTGESVPSEKDADTILPKECVLGDRSNMAYTSSIVTYGR
                     GVGVVVATGMDTEVGNIAHLLDSQDDFDTPIKRKLNTVGKTLSVIGIIVCVVIFAIGA
                     FYQRPLLPMFMIAISLAISIIPEGLPATATIVMALGVQRMAKRNALIRKLPAVETLGG
                     ATVICCDKTGTLTLNKMTVTHIAVNGDFESGKVTSIDTASEKHPLVYKEIVYAGALCN
                     DACINPDKTDEILGDPTEGALIFMAKKFGIDQEALEEEYPRVFEQPFDSDRKCMTTVH
                     NIDEKIIAYTKGAVDEILDLCTKILTSKGERNITEVDKKNIHELCLNMSKDALRVLGF
                     AKREISSIPKEDSENIEYDLTFIGMVGMIDPPRTEVIDAVSTCKEAGIRTIMITGDHK
                     VTATTIAHELGIWSEENTVISGDELDNLSDDELDEAVKNTTVFARVSPFDKLRIIQSL
                     KRIGEVPAMTGDGVNDSPALKSADIGIAMGISGTDVAKDSSDMILMDDSFTTIAYAIK
                     EGRRVYRNIQKVIQFLLVGNIAEILTLFVAILLNWDTPLLAVHILWVNLATATLPALA
                     LGVDPPSRNIMKHQPVKSTTLFEKNLIQRVVTQGIFVAIMTILAYKIGLNIENHSVGQ
                     TMAFCVLAFSQMLRAFSQRSNTDSMFNRNNGKNPFLLISFLTSAFLIAVILFVPVFRN
                     AFNLTVLNNLEWLVTIVLAIMSVIQVEVGKIIKRKKLKK"
     regulatory      4093..4195
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            4305..5570
                     /locus_tag="ACGI59_003019"
     CDS             4305..5570
                     /locus_tag="ACGI59_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase family
                     protein"
                     /protein_id="HFG9740797.1"
                     /translation="MYNKETICVQGNYKPGNGEPRVLPLYQSTTFKYSSIDQLAELFD
                     LKVDGHIYSRISNPTIQAFEEKISLLEGGVSSVAVSSGQSANMLAVLNICKSGDSILC
                     SSKVYGGTFNLLGPSLKKFGIDLISFDLDSSEDEIVELAKENTKVVFAETLANPTLEV
                     IDFEKIANVAKRINVPFIVDNSLASPVLCNPLKYGANIVTHSTTKYLDGHASSVGGII
                     VDGGNFNWDNGKFPELVEPDPTYHGISYTQKFGNAAYATKARVQLLRDYGNCLSPFNA
                     YLTNLNVETLHLRMERHSENALKIARFLEKHENVDWINYPGLEDNKYYENAKKYLSRG
                     CSGVLSFGVRGGLENAKKFVEKLQIASLVTHVSDVRTCVIHPASTTHRQLTEEQLIAS
                     GVLPSLIRLSVGIENVEDLIADLNQALNF"
     gene            5600..6490
                     /locus_tag="ACGI59_003020"
     CDS             5600..6490
                     /locus_tag="ACGI59_003020"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004414 - homoserine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019281 - L-methionine biosynthetic process
                     from homoserine via O-succinyl-L-homoserine and
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HFG9740798.1"
                     /translation="MALILPKGLPVINKLLDEGIDVIYKEDFKKELKYEENIDTKIAI
                     LNLMPIKIDTELDLLRRIDKTGFNVSVEFIKISTRESKRSCNEYVKEFYKTFDEAKGE
                     YFDGFIITGAPVEQMEFEEVDYWNELEEIMDYSKRKTKSTLYICWAAQASLYKYYNVK
                     KLPLSQKCFGVFKHKVDKDSKIVDGFENEFFAPHSRHTTVNIEALKENKELSIVSHSK
                     EAGPYIITNSRDVFVMGHSEYDKYTLDKEYKRDINKGDKISIPQNYYINDDPSEEPTV
                     KWKKHSELLFRNWIKNYLIQ"
     gene            6981..7424
                     /locus_tag="ACGI59_003021"
     CDS             6981..7424
                     /locus_tag="ACGI59_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFG9740799.1"
                     /translation="MDEMSFKKELLELTRDINMKFTTLLSDFYQPLGITAVQALILSE
                     LCEHGEKKISDLGKNLNMTNSNVSVICQRLEKNGFLNRIRDIEDQRIVKVKVTNKSLD
                     IQEHISSSIFDSYFENMTSEKLQDMEDIIEGLEKLNKLLTYIDCK"
     gene            7455..9272
                     /gene="ftsH"
                     /locus_tag="ACGI59_003022"
     CDS             7455..9272
                     /gene="ftsH"
                     /locus_tag="ACGI59_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFG9740800.1"
                     /translation="MDEHKPQKRSALYYYTVIIIVVMFLNALIFPSLMDKKMVEIDYG
                     KFLTMLDTGKVKSVEIENNRIAINPSSSKDKNIYITGRMDDPELVDRLKNAKVEFTKV
                     IPKENSPLLSILLTWIIPIGILMLFGNLMMKSMQKRMGGNAMQFGKSNAKMYVSAQSG
                     KRFNDVAGQDEAKEALTEIVDFLHNPEKYKKIGAQMPKGALLVGPPGTGKTLLAKAVA
                     GEANVPFFSISGSEFVEMFVGMGASRIRDLFKQAKEKAPCIVFIDEIDTIGKKRDNGS
                     GLGGNDEREQTLNQLLSEMDGFDGGIGVVILAATNRPDSLDKALLRPGRFDRRIPVEL
                     PDLGGREAILKVHAQNVNVSSDIDYNAIARSTSGASGAELANIVNEAALLAVKSGRDI
                     VEQQDFDESVETVIAGYQRKGAVISDKEKKIIAYHEIGHALVAAMQKHSAPVHKITII
                     PRTSGALGYTMQVAEDESVLMSKEEAIDKITTFTGGRAAEEVIFNTCTSGASNDIEQA
                     TKIARAMVTRLGMSEEFDMVALETVSNQYLGGDTSLACSSDTAAKIDEEVFKIVKACH
                     KNAIEILENNIDKLHELANYLLEKETITGDEFMEILNLN"
     gene            9450..12152
                     /locus_tag="ACGI59_003023"
     CDS             9450..12152
                     /locus_tag="ACGI59_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFG9740801.1"
                     /translation="MDRPNPDILLEKIKNEEEKLSRGQLKIFFGYAAGVGKTYSMLES
                     AQNLKKVGVDVVVGYIEPHTRPETLALLDGLEILPVKEIEYKSIKLKEFDLDAALARK
                     PEVILVDEFAHSNVAGLRHTKRWQDIEELLLAGINVYTTVNVQHLESLNDIVEIITNV
                     SVKETIPDKFLDTNTQLELIDVEPDVLLERFNEGKIYKKEQATRAKNNFFIKDNLVAL
                     REIALRKTAERVNKEVQMTRLSKGDVTVIPTSDTLLACISPSPSSAKVIRTASRISDS
                     TFAKWIALYVETPNTARLPFDEQKQLQNNLKLAKKLGGEIIVLHGENIIEQILRIAKL
                     RNVTKIVIGRNHSSNKRFSKRFKKDIVDKLIDEVDYIDIHIIPYKFASDVKYKPKKDK
                     SSIKSKFKISKVDFIKLIFITLMISILAYAVQSMGFIRENILLIYMLGVVLVSMWTKG
                     YSTGIISSVFNIVLLNYFFTAPLYTLSIADSNYIVTLVVFSIVGIITSTLTSKIQHEA
                     ETAAKREENTKMIYQIIKGFLRLSNKDDIVNKGIELLSLSLSRDVICYLNVDKHNKSK
                     LYKKNTNNKDKNDLDSEDEKAVASWVLSNSTVAGNDTDTLPGSKGYYIPIVGMNLTLG
                     VVGISCIDSKLDTEDISLIETIIAQMAIALDREILSEAKENTNLEIERERLRSNLLRA
                     VSHDLRTPLAGISGAVSTIIKNKGTIGQDIIDELLSGVYEDTQWLIRLVENLLSMTKI
                     DEGKLEVKKHSELVEEIISEALQKIKKRIENALIDINIPEQILFVPMDAKLIEQVLIN
                     LIDNSLKYSKEDCKIEINVYEKDDYVWFEVSDNGPGISKELKKHIFDRFFTGEEGAKD
                     SRKGVGLGLSICKSIIQAHKGEIMVENNKDKGSTFKFSLPKENE"
     gene            12186..12884
                     /locus_tag="ACGI59_003024"
     CDS             12186..12884
                     /locus_tag="ACGI59_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFG9740802.1"
                     /translation="MSKKLILLVEDDKTIRKFISTALLTQDYDVKEAITGKEGISIAV
                     SYSPDVVLLDLGLEDMDGIEVIKAIRQFSNIPIIVVSAREQDRDKVEVFDAGADDYLT
                     KPFSIVELLARVRVAFRHSQVEAQQKDDVKSTFEVDKLLIDFDKRKVIVDDVEVHLTP
                     IEYNILSLLAKYHGKVLTHNFIIKEIWGSVIGNETKSLRVFMATLRRKIEKQPANPRY
                     IITEVGVGYRLNDE"
     gene            13241..14197
                     /locus_tag="ACGI59_003025"
     CDS             13241..14197
                     /locus_tag="ACGI59_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HFG9740803.1"
                     /translation="MYILNLNTRETIEDFRDKFYVAENSYLILSAPKNLKLLKETLDI
                     DEITFNDCLKFDEITKLDLFDNYDFLSLNTFELRDGEAVIEEVNMYLSDNFILVVVNE
                     EHFLFEFVKNIILKNSQLEKNPVINLFKINYLILREVIKNGFESLEKVEELILQIEDE
                     MMDNINKNHVSRISDVRGLTRIIVKNTRPLLYIGDRIVKENIRYLKYSNVKKYNLENF
                     QGIDFGIDKLYNFALSTRELADKLLDIYSSRVGEKTNNLITKLTLLTAISAPLTIITG
                     IYGMNFRYMPELNWIYGYPATLFFMLCIIFVGIIIFKIKKLL"
BASE COUNT         5601 a         1594 c         2555 g         4452 t
ORIGIN      
        1 ataaaattat taataaaaac aaaaggagag atatttatgt tgagtatagg aacaaaagca
       61 ccagaattta cactagaaga taaagatgga aataaggtaa gtatgtcaga ttttaaaggc
      121 aaaaaagtag tagtatattt ttatcctaaa gataatacac caggatgtac acgtcaagct
      181 tgtgctttta gaaatgcata tgatggattt aaaaaggaag atatacaagt tattgggata
      241 agtaaagata gtataaaatc acatcaaaaa tttgcagaaa agcatgaact tccttttatt
      301 ttactttctg accctgactt agttgctata aaagcttttg atgtatggaa agagaaaaaa
      361 atgtatggaa aaactgcatt gggagttgta cgtgcgacat atattataga tgaaaatgga
      421 attattgaaa aagtctttga aaaagcaaaa ccagacacaa atgcacaaga gattcttgaa
      481 tatttggaaa aacaggagta ataagaattt atgattataa tgttatcacc agctaaaaat
      541 atgaagaata tagaagtatt tgatagagat ttgagtttgc cttgctttat agataacaca
      601 aaagaaatag tggaaaatat aaagacattt ggtatagaag attttaaaaa taaaatgaaa
      661 attaatgaaa aattagctgt attaaataaa aatagatttg aaagcattaa atttgataga
      721 ttaggaaacc ctgcaatctt aacttatgat ggaatacaat ataaaaatat agaagcagaa
      781 aattttacaa ggaaagatga agagtttgca aatagctgta ttcgaatcat aagtggattg
      841 tatggtgttg taaaaccata tgatagtata tatgaatata gattagaaat gcagacaaaa
      901 ttacaagtag gtgagtttaa gaatttatac gaatattggg gaaatcgtat atataaagaa
      961 ctaatcaaag aaaaaacagc tatcgttaat ctttcatcaa atgaatacag caaatccata
     1021 gaaaaattta ttaaagatag tgatacatat attacatgta cttttaaagt aaataaaaat
     1081 ggcatattga aagtggaatc tacacaagca aagaaagcta gaggtatgat gacaaaatac
     1141 attgtaaaaa ataggataag agatattgaa gaactgaaaa agtttgattt agaaggttat
     1201 aagtataaag aaaacttatc taacaatagt gaatacatat ttgtaaaaga gtagaagttt
     1261 tgttaaatat taaatggcat tgttaaattt aggtaaaata tacttagtgt gtattgaatt
     1321 gaacttgatt ttaaaatata attattctta aattaaaaaa agaaaggagt ggtcttccaa
     1381 aatggaaaat cacaaacaga tgccttggca ttcaagagca acgcatgaaa ttttagaaag
     1441 tctagattca tcagaggagg ggctcaccga taccgaggcg gaaaaacggc ttaaagagaa
     1501 tggcttaaat gaacttagaa aaaaaccacc tagaacaact ttacaaatgc tttggagtca
     1561 aattacagat gctatggtta tgatactaat aggagcagca atattatctt tgatatttgg
     1621 cgaatttaca gaagcttttg taatactaat aattgtaaca gtaaatgcag ttattggaat
     1681 tgttcaagag aaaaaagcag aatcatcatt agaagcatta aaaaatatga attcacctac
     1741 agctcgtgtt atgcgtgatg gtgaagaaaa tgttattcca gcaagtaatc ttgtagtagg
     1801 agatattgtt tttttagaag atggagctat ggttccagca gaccttcgtt taatagaaac
     1861 atcaagttta aaaattcaag aggcttcttt aacaggagaa tctgttccat cagaaaaaga
     1921 tgcagataca atcttaccta aagaatgtgt attaggagac cgtagcaata tggcgtatac
     1981 atcttcaata gtcacatatg gtagaggtgt aggtgttgta gtagcaactg gtatggatac
     2041 agaagtgggt aacatagcac atcttttaga ttcacaagat gattttgata ctcctataaa
     2101 acgtaaactt aatactgttg gaaagacatt aagtgtcata ggtattattg tatgcgttgt
     2161 tatatttgca ataggtgcat tttatcagag acctctcttg ccaatgttta tgattgcaat
     2221 ttcccttgcc atatcaatta ttccagaggg attacctgca acagctacta ttgtaatggc
     2281 tttaggcgta cagcgtatgg ctaaaagaaa tgctttaatt agaaaattac ctgctgttga
     2341 aacattagga ggagctacag taatatgttg tgataaaact ggtacattaa ccttaaataa
     2401 aatgactgtt acacatattg ctgtcaatgg agattttgaa tctggaaaag tgacatcaat
     2461 agatactgca tctgaaaaac atccattagt gtataaagag attgtttatg ctggtgcact
     2521 ttgtaatgat gcatgtatta atccagataa aacagatgaa atattaggtg acccaacaga
     2581 gggcgcatta atttttatgg ccaaaaaatt tggaatagat caagaagctc ttgaagaaga
     2641 atatccacgt gtatttgaac agccttttga ttcagataga aaatgtatga caactgttca
     2701 taatatagat gaaaagatta ttgcttatac taaaggagca gtagacgaaa ttcttgacct
     2761 atgtactaaa atattaactt caaaaggtga aagaaacatt actgaagtgg ataaaaagaa
     2821 tatacacgaa ttatgtctta acatgtctaa agatgcattg cgtgtacttg gttttgctaa
     2881 gcgtgagatt tcatctatac caaaagaaga tagtgaaaat atagaatatg atttaacatt
     2941 tataggtatg gttggtatga ttgacccacc aagaactgag gtaattgatg cagtaagtac
     3001 ttgtaaagaa gcaggtataa gaaccataat gataacaggt gaccataaag ttactgccac
     3061 aacaatcgct catgagttag gaatatggag tgaagaaaat actgtgatat ctggtgatga
     3121 acttgataat ttgagtgatg atgaactaga tgaagctgtt aaaaatacta cagtatttgc
     3181 tcgtgtatcc ccatttgata aacttagaat tatacaatct ctaaaaagaa ttggagaagt
     3241 accagcaatg actggggatg gtgtaaatga ctctcctgcc ttaaagtctg ctgatattgg
     3301 tatagccatg ggtataagtg gtacagatgt tgcaaaagat tcttctgaca tgattttaat
     3361 ggacgatagt tttacaacaa ttgcttatgc aataaaagaa ggtagacgtg tatatagaaa
     3421 catacaaaaa gttatacaat ttttgcttgt tggtaatata gcagagatac ttacgttgtt
     3481 tgtagcaata ttattaaatt gggatactcc gcttcttgct gttcatattc tatgggtaaa
     3541 ccttgcaact gctacgcttc cagcccttgc tcttggagtt gacccaccaa gcagaaatat
     3601 aatgaagcac cagccagtga aatcaaccac tttgtttgaa aaaaatctta tacaaagagt
     3661 tgttacccaa ggtatatttg tggcaataat gactatttta gcatataaaa ttggtcttaa
     3721 tatagaaaat cattctgtag gtcaaacaat ggcattttgt gtattagcat tttcacagat
     3781 gttacgagca tttagtcagc gttcaaatac agattcaatg tttaatagaa ataatggtaa
     3841 aaatccattt cttttaatct catttttaac ttctgcattt ttaatagcag taatactatt
     3901 tgtacctgtt tttagaaatg cttttaactt aacagtttta aataacttag agtggcttgt
     3961 aacgattgtt ttagccataa tgtctgtaat tcaagtagag gttgggaaaa ttattaaaag
     4021 aaaaaaatta aaaaaatagt tgacaaaatt aggaaaaata tataagatat aaaacataga
     4081 aaaattaaat aaatcttatc cagagaggtg gagggactgg ccctatgaaa cccggcaacc
     4141 agtatattta tatacattgg tgctaaatcc tgcggcgaaa gctgagagat gagtataatt
     4201 aagcttatac agagcctgag ttatatactc aggctttatt tattttaact ctcatctggt
     4261 aaatagttta atttttttta ctaaaatcta agggggatac aagaatgtat aataaagaaa
     4321 caatatgtgt gcaaggaaat tataaaccag gtaatggaga accaagagta ctacctttat
     4381 atcaaagtac aacttttaaa tatagcagta tagaccaact tgctgaatta tttgatttaa
     4441 aagttgatgg acatatatat tcaagaataa gcaatcctac tattcaagct tttgaagaaa
     4501 aaataagttt actagagggt ggagtatctt ctgtagctgt atcatcaggg caatctgcaa
     4561 atatgttggc agttttaaat atatgtaaat caggagatag tatactttgt tcttcaaaag
     4621 tatatggagg aacattcaat ttactaggac ctagtcttaa aaaatttggt atagatttaa
     4681 tatcgtttga cttagattca agtgaagatg agatagtaga acttgcaaag gaaaatacta
     4741 aggttgtgtt tgcagaaaca cttgcaaatc caactcttga agtcatagat tttgaaaaaa
     4801 tagcaaatgt agctaagaga attaatgttc catttattgt tgataattca ttagcatctc
     4861 cagtgctttg taacccttta aagtatggag caaatatagt tactcattct accacaaaat
     4921 atttagatgg gcatgcttca agtgttggag gaattatagt ggatggtgga aactttaact
     4981 gggataatgg aaaatttcca gaattagttg agccagaccc aacatatcat ggtataagct
     5041 atactcaaaa atttggaaat gccgcatatg caactaaagc aagagttcag ttgcttagag
     5101 actatggaaa ttgtttaagc ccattcaatg cgtatcttac taatttaaat gttgaaacac
     5161 tacatcttag aatggagaga catagtgaaa atgcacttaa aatagctaga tttttagaaa
     5221 aacatgaaaa tgtagattgg attaattacc caggacttga agataacaag tattatgaga
     5281 atgccaaaaa gtatttatca agaggatgta gtggtgtttt atcatttgga gtaagaggtg
     5341 ggttagaaaa tgccaaaaaa tttgtggaaa aattacagat agcatctttg gttacacatg
     5401 tttcagatgt aagaacttgt gttatacatc cagcttcaac tactcataga caattaacag
     5461 aagaacaatt aattgcatct ggagtattgc cttcactaat aagattatct gttggaatag
     5521 aaaatgtaga ggatttaata gctgatttaa atcaagcttt aaatttctaa tattctaaga
     5581 gaaagagaag tgaaaagata tggctttaat actaccaaag ggattacctg ttattaataa
     5641 attattagat gaaggaatag atgtaattta taaagaagat tttaaaaagg aattaaagta
     5701 tgaagaaaac atagacacta agatagctat actaaatcta atgcctataa agatagatac
     5761 agaattagat ttactaagaa ggatagataa aacaggattc aacgtgtctg tggaatttat
     5821 taaaatttca acaagagaaa gtaaaagaag ttgtaatgaa tatgttaaag aattttataa
     5881 aacatttgat gaagccaaag gagagtattt tgatggcttt ataataacag gagctccagt
     5941 tgaacaaatg gagtttgaag aagttgatta ttggaatgaa ctagaagaaa ttatggatta
     6001 ttctaagaga aaaactaaat ctacattata tatatgttgg gcagctcaag caagtcttta
     6061 caaatactac aatgtaaaaa aacttccact aagtcaaaaa tgttttggag tatttaagca
     6121 taaggtagat aaagatagta aaatagtaga tggatttgaa aatgaatttt ttgctccaca
     6181 ttcaagacat actactgtaa atattgaagc tttgaaagag aataaagagt tgagcattgt
     6241 gagtcattct aaagaagctg gtccctatat aattacaaat tcaagagatg tgttcgtaat
     6301 gggtcatagc gagtatgaca aatacactct tgataaagaa tataagaggg atataaataa
     6361 aggagataaa atatctatac ctcaaaatta ctatattaat gatgaccctt cagaagagcc
     6421 aactgtaaaa tggaaaaaac attcagaatt gttgtttaga aattggataa aaaactattt
     6481 gatacaataa attttaagag tatataaatg ttaactaaaa aaatatagaa atttataggt
     6541 ataaagttaa tcttaatgat actttatacc ttattttttt aaaatttgac cttataatag
     6601 tagttttata ttttaagaaa atcactttag gtgtatgtag atagaggtgg ctttaatttt
     6661 atatatggtg cgataatggg agactaaacc tttatagtat caataaattt gtattttaac
     6721 ttttaaaaga gattaaaaag ggagaaatca gtgtaaaata tatatataga ttttttatta
     6781 aattttatta aaaatatcct gtataaaatt aaatatcaat aagataacaa aatttcaatt
     6841 gaagttacaa tattgtaatt gacaataaat acttaatact ataatattta atacattaaa
     6901 cattatagtg ttaagtattt atttacagta tgcacttcag cctaaatttg aaataaagta
     6961 ttttcgtagg gagcgtttgt atggatgaga tgagttttaa gaaagagcta ctagagttga
     7021 ctagagatat aaatatgaaa tttacaactt tattaagtga tttttatcag ccacttggta
     7081 ttacagcagt gcaagctttg attttatctg agttatgtga acatggagag aaaaaaataa
     7141 gcgatttagg gaagaactta aacatgacaa atagtaatgt atcagtaatc tgtcaaaggc
     7201 ttgaaaaaaa tggcttttta aatcgcataa gagatataga agaccaacga attgtaaaag
     7261 taaaagttac aaataaatca ttggatatac aagaacatat ttcaagtagt atttttgata
     7321 gttattttga aaatatgacc agtgaaaaat tgcaagatat ggaagatata atagaaggtt
     7381 tagagaaatt aaataaactt ttaacttaca ttgattgcaa ataacataaa aataatttaa
     7441 aggagaataa aattatggat gaacataaac cccaaaaaag gtcagcactc tattattata
     7501 cagttataat aatagtagta atgtttttaa atgctttaat atttccaagt ttaatggata
     7561 aaaaaatggt tgagattgac tatggaaagt ttctcacaat gttggataca ggaaaggtta
     7621 aatctgttga gatagaaaat aatagaatag caataaatcc atctagtagc aaggataaga
     7681 atatatacat aactggtaga atggatgacc cagaattggt agatagacta aaaaatgcaa
     7741 aagtagaatt tactaaggtt attcctaagg aaaattcacc tcttttaagt attttactta
     7801 catggataat acctataggt attcttatgc tatttggtaa cttaatgatg aagtctatgc
     7861 aaaagcgaat gggtggtaat gctatgcaat ttggaaaaag caacgccaaa atgtatgtat
     7921 ctgctcaaag tggaaaaaga tttaatgatg tagctggtca agatgaggca aaagaagctt
     7981 taactgaaat agtagatttc ttacacaatc cagaaaagta taaaaagata ggagcacaaa
     8041 tgcctaaagg agcactttta gtaggacctc caggtacagg aaaaacactc cttgcaaaag
     8101 ctgtagcagg tgaagcaaat gtaccatttt tctcaatatc tggttctgag tttgttgaga
     8161 tgtttgtagg aatgggagct tctaggattc gtgatttatt taaacaagca aaagaaaaag
     8221 ctccatgtat agtgtttata gatgagattg atactattgg taaaaagcgt gataatggaa
     8281 gtggattagg tggaaatgat gaacgtgaac aaactttaaa tcagttgtta agtgagatgg
     8341 atggttttga tggtggaatt ggagtagtaa tacttgctgc tacaaataga cctgacagtc
     8401 ttgacaaagc attacttcgt ccaggaagat ttgatagacg tataccagtt gaacttccag
     8461 accttggagg acgtgaagct atattaaagg tacatgctca aaatgtaaat gtaagctcag
     8521 atatagatta taatgcaatt gcaaggtcta catcaggagc atcaggtgca gaacttgcaa
     8581 atattgtaaa cgaagcagca cttttagcag ttaagtctgg cagagatata gtagaacaac
     8641 aggattttga tgaatctgtt gaaactgtaa ttgctgggta tcaacgtaaa ggagctgtaa
     8701 tttcagataa ggaaaagaaa attatagcat atcatgaaat aggtcatgca cttgttgctg
     8761 ctatgcaaaa acattctgca cctgtccata agattactat aatacctaga acttcaggag
     8821 cattgggata tactatgcaa gttgctgaag atgagagtgt acttatgtct aaagaagaag
     8881 caattgataa aataactact tttacaggtg gtcgtgctgc tgaagaggtt atctttaaca
     8941 cttgtacatc aggagcatca aatgatatag aacaagctac taaaattgca agagctatgg
     9001 ttactcgcct tggtatgagt gaagagtttg atatggtcgc tcttgaaact gtaagcaatc
     9061 aatatttagg aggagatact tctcttgctt gttcttccga cactgctgct aaaatagatg
     9121 aagaggtatt taaaatagta aaggcttgtc acaaaaatgc aattgaaatc ttagaaaata
     9181 atattgataa attgcatgaa cttgctaatt atttattaga aaaagaaact attacaggtg
     9241 atgagtttat ggaaatactt aacttaaact aaaatgatta tacacaagat aaaaatgcct
     9301 attgttaggc atttttattt tacaactaaa ttttttaaat ttatatttaa atctttacat
     9361 aatcttaatc attaaaaata catcttaact acaattttat atcggaattg atattataat
     9421 agtaatgaat ggtgtaagga ggttttttaa tggatagacc taatcctgat attctattag
     9481 aaaaaattaa aaatgaagaa gaaaaactaa gtagaggtca attaaaaata ttctttggtt
     9541 atgcagctgg agtaggaaaa acctactcta tgttggaatc tgcacaaaat ttaaaaaaag
     9601 tgggtgtaga tgtagtagtt ggatatattg aaccacacac tagaccagaa acattggctt
     9661 tacttgatgg cttggaaatt cttcctgtaa aggagataga atataaatct ataaaactaa
     9721 aagaatttga tttagatgct gctttagcaa gaaaaccaga agttatatta gtagatgaat
     9781 ttgcccattc taatgttgct gggttaaggc atacaaaaag atggcaagat atagaagaat
     9841 tgctgttagc aggcataaat gtctacacta ctgtaaatgt acaacattta gaaagtctaa
     9901 atgacattgt tgagataatt acaaacgtat cagttaaaga gactatacca gataagtttt
     9961 tggatacaaa tacacagtta gaacttatag atgttgaacc tgatgtactt cttgaaaggt
    10021 tcaatgaagg taagatatat aagaaagaac aagctactag agcaaaaaat aattttttta
    10081 taaaagataa cctggttgca cttagagaaa ttgcacttag aaaaacagca gagagagtta
    10141 ataaagaagt tcaaatgacc agactatcaa agggtgatgt aactgtaata cctacatcag
    10201 atacacttct tgcatgtata agtccatctc catcttcagc aaaagttatt agaactgcat
    10261 ccagaatatc agacagtaca tttgcaaaat ggatagcttt atatgttgaa actccaaaca
    10321 cagcaagact tccatttgat gaacaaaaac aactacaaaa taacctaaaa ttagctaaga
    10381 aattaggtgg agaaataatt gttttacatg gtgaaaatat aatagagcaa atacttagaa
    10441 ttgcaaagct gagaaatgta acaaaaattg taataggtag aaatcattca agtaataaaa
    10501 gatttagtaa aagatttaaa aaagatatag ttgacaaatt aattgatgaa gttgattata
    10561 tagatataca tataattcca tataaatttg cctcagatgt taaatacaaa cctaaaaagg
    10621 ataaatcatc aataaaatct aaatttaaaa ttagcaaagt tgattttata aaacttatat
    10681 ttattacttt gatgataagt attttagcat atgcagtaca aagcatggga tttataagag
    10741 aaaatatatt gttaatatat atgttgggtg tagttttagt ttctatgtgg actaaagggt
    10801 atagtacagg tataataagt tctgtattta atatagttct tttaaattat ttttttacag
    10861 caccattata tacattatct atagcagatt caaactatat agtaacactg gttgtattct
    10921 caatagtagg tataataact agcactctta cgtctaagat acagcatgaa gcagaaactg
    10981 ctgctaaaag agaagaaaat acaaaaatga tatatcaaat aattaaggga tttttaagat
    11041 tatcaaataa agatgatata gtaaacaaag gtatagaact tttgtctctt agcttaagta
    11101 gagatgtaat ttgttattta aatgtagata agcataataa atcaaagctt tacaaaaaaa
    11161 atactaataa taaggataaa aatgatttgg atagtgaaga tgaaaaagct gttgcaagtt
    11221 gggttttatc caattcaaca gtagcaggaa atgacacgga tacacttcct ggttcaaagg
    11281 gatactatat accaattgta ggaatgaatt taactttagg agtagtggga atatcatgta
    11341 ttgattcaaa attggataca gaagatataa gcttaattga aacaataatt gctcaaatgg
    11401 caatagcgct tgatagagaa atcttgtcag aagctaaaga aaataccaat cttgagattg
    11461 aaagggaaag attaagaagt aatcttttaa gagcagtttc gcatgacctt agaactccac
    11521 tagcaggaat atctggtgca gtaagtacaa ttataaaaaa caagggtact ataggtcaag
    11581 atataataga tgaattactt agtggtgtat atgaagatac tcagtggtta ataagacttg
    11641 ttgaaaattt acttagtatg accaagatag atgaagggaa attggaagtt aaaaaacatt
    11701 ctgaactggt tgaggaaatt atatcagaag cgttacaaaa aataaaaaaa agaattgaaa
    11761 atgcacttat agatattaat ataccagaac agatattatt tgttcctatg gatgcaaaat
    11821 taatagaaca agttttaatt aatttaatag ataattcttt gaaatattca aaggaagatt
    11881 gtaagataga gataaatgta tatgagaaag atgattatgt ttggtttgaa gtatctgata
    11941 atggtcctgg tatatcaaag gaacttaaaa agcatatctt tgacagattt tttactggag
    12001 aagaaggagc taaagattcc agaaagggtg ttggacttgg attatcaatt tgtaaatcta
    12061 taattcaagc tcataaaggt gaaattatgg tagaaaacaa taaagataag ggttcaactt
    12121 ttaagtttag tttgcctaag gaaaatgaat agttttaaat tttaaaatta gttttggagg
    12181 aagaaatgag taaaaaactt atattgttag tagaggatga taagacaata agaaaattta
    12241 tatctactgc acttttaaca caggattatg atgtcaagga ggctataact ggaaaagaag
    12301 gtatatctat agctgtctca tattctccag atgttgttct tttagattta ggcttagaag
    12361 atatggatgg aatagaggta ataaaagcta tcagacaatt ttcaaatata ccaataattg
    12421 tagtttcagc tagagagcaa gatagagaca aggtagaagt atttgatgca ggggcagatg
    12481 attacttaac aaaaccattc tccatagtag agttattagc aagagtaaga gtagcattta
    12541 gacattcaca agtagaagct caacaaaaag atgatgttaa atctacgttt gaagtagata
    12601 aattattgat tgattttgat aaaagaaaag ttattgttga tgatgttgaa gtccatttga
    12661 cacctattga atataatatt ctttcattac ttgcaaagta tcatggaaaa gtgcttactc
    12721 ataattttat aataaaagaa atatggggta gtgttatagg gaatgagact aagtctttaa
    12781 gggtctttat ggcaacgtta agacgaaaaa tagaaaaaca acctgcaaac ccaagatata
    12841 taattacaga agttggtgta ggatacagat taaatgatga atagttgtaa tatattaatt
    12901 attagattat gtgcttatta atatatatta aaattatata gtttagttaa aataagaatg
    12961 aagtgtatgt gcttgttgtc agtttagcac atgcatttta ttttttagct tttttagtat
    13021 caataatttt ttagtatcaa taatatgaaa tatgttttaa aataagtata tagtgtaata
    13081 tgtaataaaa actttggtta aataataaat gtatttaaca cttaccttat aataaattta
    13141 atataaatat taaataataa ttattaaaat agtgtaaaat aattacagta aggattataa
    13201 aaaattttta gaatgtaaat tgaaatttag gagtagatgt atgtatatac tgaatttaaa
    13261 tacaagagag actattgaag attttagaga taaattttat gtagcagaaa attcttatct
    13321 tatattaagt gctccaaaaa atttaaaatt gcttaaggaa actttggata tagatgaaat
    13381 aacttttaat gattgtctta aatttgatga aataaccaaa cttgatttat ttgataatta
    13441 cgatttttta agcctaaata catttgaact aagagatgga gaagctgtta tagaagaggt
    13501 aaatatgtat ttatcagata attttatatt ggttgtagtg aatgaagaac actttttatt
    13561 tgaatttgta aaaaatatta ttttgaagaa ctctcaactt gaaaaaaatc cagttattaa
    13621 tctatttaag ataaactact tgattcttag agaagttata aaaaatggtt ttgagtcttt
    13681 ggaaaaagtt gaagagttaa ttcttcaaat agaagatgaa atgatggaca acattaataa
    13741 gaatcatgta tctagaataa gtgatgtaag aggattaaca agaattatag ttaagaatac
    13801 tagacctctt ttgtatattg gggatagaat agttaaggaa aatataagat atttaaaata
    13861 ttcaaatgta aaaaaatata atttagaaaa ttttcaaggt atagattttg gaattgacaa
    13921 attatacaat ttcgcattgt caacacgaga gttagcagat aaattattag atatttattc
    13981 ttccagagta ggtgagaaaa caaataactt aattacaaaa ttaaccttgt taacagccat
    14041 atcagcacca cttacaatta taactggtat atatggaatg aactttagat atatgccaga
    14101 attaaactgg atttatggat atccagcaac tttatttttt atgctatgta taatttttgt
    14161 tggaattata atatttaaaa taaaaaaatt attgtaaatt ac
//
LOCUS       DAYAPA010000115        14140 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.117, whole genome shotgun sequence.
ACCESSION   DAYAPA010000115 DAYAPA010000000
VERSION     DAYAPA010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14140
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.117"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            402..1805
                     /locus_tag="ACGI59_003026"
     CDS             402..1805
                     /locus_tag="ACGI59_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFG9740804.1"
                     /translation="MKMLNNFFKKINNPAPVFAQTKMERDNQTDDSLSTKPKTTFRDV
                     AGLDEVKEELFEIVDFMKSPQKYQKMGAKIPKGVLFYGPPGTGKTLLASAVAGETNSS
                     FFNVTGSEFVEKYVGVGAKRVRTLFEKARKEAPSIIFIDEIDAVGAKRHLESNNEKDQ
                     TLNQLLVEMDGFNKDSNVLIIGATNRLDLLDEALLRPGRFDRHIHIGAPNYHTRFEIL
                     KVHTDDKPIDKSVNLELLAKKTHGFNGAHLSNIANEAAIFAVRDDSECITSEHFDKAL
                     ERVIAGLESKNSALVEKEKKIVAYHEAGHALVSDIVGICPIQKISIVPRGQALGYVLQ
                     LPDEDRYIYTKDELIGKIKILLAGKASEELIFNHKSTGAKDDLKKVTEIANQMVCEYG
                     MSNLGFMTIDGNDKTFLCDKVQKEANRIVEICYKETLEMLKDNLEDLHSVSKFLFEKE
                     TMTHEELKDLIGKEAVN"
     gene            complement(2282..3304)
                     /locus_tag="ACGI59_003027"
     CDS             complement(2282..3304)
                     /locus_tag="ACGI59_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFG9740805.1"
                     /translation="MNQKKVHYNKSATSKDVARLAGVSQSSVSRAFGSANGKGVKPEV
                     RERIFRVANEIGYVPNLVARGMISGKTNVIGLIVGDSLGPFYNRIINLFVEKIQEIGK
                     QCLVFKVPRQQSIDSIISKVIQFQVEAVIITASAMNKVMAETCEENNIPVILFNRFIP
                     GIKISTVYVDPIEGGGMAAEYLLKKGHKNIGYIQFTRETSEEMEKKIGFYSKLRQSGI
                     HNLKEESANYDYDSGYEAGKRMLALSNPPTAIFCTSDLIAIGVMDAARFEYKLRVPED
                     LSVIGYDDIQMASWKSYNLTTIRQPLDLLIEKTINILKSLLNEENPESVVEMLKPELI
                     ERGSTL"
     gene            complement(3339..4937)
                     /locus_tag="ACGI59_003028"
     CDS             complement(3339..4937)
                     /locus_tag="ACGI59_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HFG9740806.1"
                     /translation="MISSIDVCIIIGYLLLMLVIGYYSGKDNKNQEDYFLAGRSMPWI
                     PIALSVAATMISANGFIGGPGWAYVDGMYPVMVNITVPLAIFFALSITTPVIYKLKIT
                     SIYEYMGLRLGNYSRGLTIIQFFINSLIQASSMVYIPVLIIQMITGWSLYALVPIVVL
                     VSIIYTLLGGIKAVIWTDSIQMIVVISAVFIVIIVALKGMNLSFFDTLQIAQQSGKLN
                     TFDFSRDISVTNTFWATLIGGTVMWIRYFCFDQSQVQRVLTSKSLKCAKNSFVVSAFV
                     MNLVYYFMLLVGVILFVFYGGRTFETSNEIMITFILNELPVGAIGIIIAGVFAAAMSS
                     IDSILNSMTTVFTKDIYEFYFKKDNKESSLKVTMIITVVIGVVMTGFIIMGFGGSIKS
                     ILDLVGNYISYFAGPATGAFVLAMFTYKANDKGVSIGFIVGLVLGFFISNKYNVSWLW
                     NPAIGATITLIFGYIFSVILKKNHNADDIKKYTAFGMRNHLISQGMDKVDGVSIVPFS
                     LGKQELIVLAFFIIQYIILFLIQF"
     gene            complement(4979..6244)
                     /locus_tag="ACGI59_003029"
     CDS             complement(4979..6244)
                     /locus_tag="ACGI59_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFG9740807.1"
                     /translation="MKTTVEIENDIILGKDYSNLKRGIAFFSIALIYFFYCYNFMVGT
                     FIKPTMIYALADGGFGFSLKQTEEIFAVMSFGTIPGTFIFGVISTKIGKKRTLISVAL
                     LIGLTTFIPMLSPTNIMLWKIARLCTGVVLGGVFGTAMPLVADMFPSKYRGKLAAILT
                     ALFSLAMIFGGKVYGVLGDANWQILMYTAIIPPIVGAILAIFFVPDDLEYTKELVKKG
                     KETGEKISYISMYKGKYLWIGLGTILLSGANFTAYSAFSNNATTYLVTGIGMSAAVAE
                     SIYSLQGIGQLVGYLFWGSIADKFGRKIPFIGMALAAVFVFIYTKLGGDNTTTFYMIS
                     VLLGVAVGYSGAWGAYYTELFPSKYRSLSSGMSFNGGRIISTFAIPAIAGTASGTLGM
                     MTIFYISIAVFVAGAIVWLFLPETLNNKK"
     gene            complement(6386..7813)
                     /locus_tag="ACGI59_003030"
     CDS             complement(6386..7813)
                     /locus_tag="ACGI59_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HFG9740808.1"
                     /translation="MASTNKNNATSKEEEKEVSTSGLESCTVKENKAKMNQTNFSNIN
                     ENQTNENKTNTYKRNIDKGNIDKGNIDKTNTNKSDINKVNIDTVDTDKLNTNTLTEKN
                     KKVIFKGEDNMSSTKIYYGDSLEVTPVGVMDYNDFSKQTKREQEIPGFDSKYRDFVDY
                     IMKITHNIWEEKGIGVIYDTYHNNVTMHCGSSNLVGIKDVISNTLQTLHAFPDRRLIG
                     QNVIWSNFGADGFLSSHRVLSTATNLGDSNFGPATGRKINFRTVIDCATTNNRIHEEW
                     LVRDNLWIVTQLGLNPQEVAKSMAKASESKVLSLQSTYGICESMDGQFMPTKYQAKDD
                     SVGEMMLEMTSRIYNYKYINEVKKYYHDNAVVHFICDKDLNGYDEIQGMIVSLFASIP
                     NGSYEVERVTCNQREKNEGYDVSVRWRLRGINEGIGFFGQPSGKHIEVMGINHYHILQ
                     GKVKEEWITFDGMDVLKQIYMGVEE"
     gene            complement(7851..8858)
                     /locus_tag="ACGI59_003031"
     CDS             complement(7851..8858)
                     /locus_tag="ACGI59_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HFG9740809.1"
                     /translation="MGKYQDSKKVVRNYFEALENATGDEVENVLKQHMKSDYNWKGVY
                     PFREQEGTENVADVFWKPLKKSLSNMQRRQDIFIAGTNEIDNSEWVMSMGHFMGLFDN
                     DWLGIKHTRKMISLRYAEFNCVQDGKIAKTGLFVDIIGFMIQAGVNPLPPQTGSYFVY
                     PGPIDHNGLLFEDADEVEGVKTLALVNKMVDDLSELNESGAMGCPPEILEKSWAKDMI
                     WYGPGGIGASYTIPRYQEQHQLPFRNNLKGKTFNGHVCRFAEGNFACFFGWPNLTNVP
                     CGGFLGLPGGEVKADMQVVDVYYRKGDKLQENWVLIDIPYWLKQQGLDILERTKSIFN
                     A"
     gene            complement(9055..10377)
                     /locus_tag="ACGI59_003032"
     CDS             complement(9055..10377)
                     /locus_tag="ACGI59_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9740810.1"
                     /translation="MKILYVGPGMVIDKILAVSKKNFPNIEADFLRYDRYIEVPDLLE
                     KYRGRVDAVLFTGKSPFKFFEKRQKDFVLSDYIPRHETTLYRVLLEITYLLKFDISRL
                     SIDTYDKAMMKRLYKEIGVEFKDEHMFFAEQKYLEEDYIKFVLEFHKYNYQNNDVCCC
                     ITGLEEAYHIMKKDNIPVVLALPSEDVMVQSIKNIQMRYIAKKNNENQIIVLAIKLNM
                     PSEYSLMKEDEYAYLSQRIKILDKLYHFNSRIDGVLVEQSRSEFMIFTTKKILELETK
                     KYRDIYLLDMIREVSVINTYIGIGYGKTANEAKFNAYESIKMAQSQKNDAAYVVFENG
                     EIMGPLKPNPTVKEKNSFDERFYQIATETGLSANTVYKVFNKIINSGKTEFTSKELAT
                     ICGVSIRTMDRIILRLCDSGYCEVVSEQLMSKYGRPSRILRFKPFLLF"
     gene            complement(10735..12375)
                     /locus_tag="ACGI59_003033"
     CDS             complement(10735..12375)
                     /locus_tag="ACGI59_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFG9740811.1"
                     /translation="MFDVKIVNGIIVDGTGNSRYKSDVGIVGDKIMAIGDLSQKEAKE
                     TIDATGKFVSPGFIDFHTHSDLSLVYDKYTRSRIHTGVTTDVIANCGIGVAPIREEKK
                     QELIDYLGTRIIGTIPTKLELHWNTMQEYFDYLTENSPAVNVVAYVAQGPVRINEMGF
                     SKEPATLEQLKNMKLEVRKAMEAGCVALSSGLVYLPGAYTKKEEMAELCKELIPYNGY
                     YISHIRDEGDEEMEALDEAIYIAKTAGVPLHISHLKVMGHKNFGTIDEVFKKLDEAEA
                     DGLEVTFDCYPYTAGMTSLGALLPPWAFEGGVENMVKRLEVQENRDRIIKELEEGIPG
                     WQCFYQLAGGWNGVVLASVMTEANKYVEGKTLMEVAKINGENPFDTFFRLLIEEKSKI
                     QVVVHTQGQEDTDKVVCHPKSCIGSDSMDLSTEGLLSLGKPHPRAFGTFGRIFSYYVR
                     EKGMLTFEEAVKKITYLSAKRLGIYKERGLLKENYFADIVVFDPDTIEDKATYSNPKQ
                     YTVGVEYVLVNGKIALAGGKQTDVCAGRVIKNPLSIAK"
     gene            complement(12531..13187)
                     /locus_tag="ACGI59_003034"
     CDS             complement(12531..13187)
                     /locus_tag="ACGI59_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9740812.1"
                     /translation="MYDKKMIKIGRISIGLAIIANFIPAIYVGIRFGQMPMLSVIFQI
                     WGLVAATYGVSWIVQPIAYYPTLGAPGSYIGWLAGSVGDIRMPAASMAQKIAGVEAGT
                     HEGEVVGTIGTCCSIFVSASMITIFTIVGYKVIPLLPEFVTNSFNYILPALFGAIYVD
                     IARKDIRAGGCTIAVALAIMYFGEKIGIPGGLLTLFIVLGGILITRVFFVIDNNKTKE
                     "
     gene            complement(13213..13914)
                     /locus_tag="ACGI59_003035"
     CDS             complement(13213..13914)
                     /locus_tag="ACGI59_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5058 family protein"
                     /protein_id="HFG9740813.1"
                     /translation="MKFQEMINSPGLWIVSSFLVIISVVQAIVFMKEALKEASNLGIE
                     KKGIKAAIRSASVTAIGPSLSPVITLLSLVAVIGAPTTWMRLCDVGAARTELGVISLT
                     SNLSGVEVGSAAFGAEAFSYALWGMALNNLGWLFVVFILGHRMRGIVEKMNMKYNPTW
                     VKKLLAGATVGLFAYLLSNQIKTFEIPKLTPAIISAIVMLVLTTLFKKNQRLQELSLG
                     IAMLIGMFGTQIILS"
BASE COUNT         4805 a         2660 c         1672 g         5003 t
ORIGIN      
        1 aacaaatcct ataattatat tctaatatac tacctactac tttgcgctac aacaatttct
       61 ctatttatat gatttgtaaa atatagtaca acatttttta tcattttcat ttcaactaaa
      121 tttgtaatat tttattcaat acttaataaa tacttaatca atacatgctt tataatactt
      181 tactcaatat ataatcaata cataatttat aatttgtaat actttattca atacataagt
      241 agaaatctct atatacataa attcattttt tcatacatct tacaaattta tttttattta
      301 gataacatgt atattttttc catgaaaaat aaataataaa aaatagtaaa tatggaaaat
      361 ataaaattga ttaacaataa ttaattacct gaggtgtgaa tatgaaaatg ctaaacaact
      421 tttttaagaa aatcaacaat ccagctcctg tctttgcaca gacaaaaatg gaaagagata
      481 atcaaacaga tgattcactt tctacaaaac caaagactac atttagagac gttgctggat
      541 tagacgaagt aaaagaagaa ctatttgaaa tagtcgattt tatgaagtca ccacaaaaat
      601 atcaaaaaat gggtgcaaaa atacctaaag gagtattatt ttatggtcct ccaggaacag
      661 gtaaaaccct acttgcatct gctgtagctg gtgaaacaaa ctcatcattt ttcaatgtta
      721 ctggttcaga gttcgttgaa aaatatgtag gtgtgggagc aaaaagagtt agaactctat
      781 ttgaaaaagc tagaaaagaa gctccaagta taatatttat agacgaaata gatgctgtag
      841 gagcaaagag acatcttgaa agtaacaacg aaaaagacca aacactaaac caacttttag
      901 ttgaaatgga tggatttaat aaagattcca atgtattaat aataggtgct accaatagac
      961 ttgacttatt agatgaagct ttacttagac caggaagatt tgacagacac attcatatag
     1021 gtgcaccaaa ctatcacact agatttgaaa ttctaaaggt acatacagat gacaaaccta
     1081 tagacaaatc tgtaaatcta gaacttttag ctaaaaaaac tcatggtttc aatggggctc
     1141 atctttcaaa tatagcaaat gaagcagcta tatttgcagt tagagatgac agtgagtgta
     1201 ttacatcaga acattttgac aaggcacttg aaagagttat tgcagggcta gaatctaaaa
     1261 actctgcatt ggttgaaaaa gagaaaaaaa tagtagcata tcatgaagct ggacatgctc
     1321 ttgtaagtga tatagtagga atttgtccta tacaaaaaat atctatagtt cctagaggac
     1381 aagcattagg ttatgttctt caattacctg atgaagatag atatatttat actaaggatg
     1441 agttgattgg aaaaataaaa atattacttg ctggaaaggc ttcagaagaa ttaatattta
     1501 atcataaatc tactggtgca aaggacgact taaagaaagt cactgaaata gctaaccaaa
     1561 tggtttgtga atatggtatg agtaacttag gatttatgac tattgatgga aatgataaga
     1621 ctttcttatg tgataaggtt caaaaggaag caaatcgtat agttgaaatt tgttataaag
     1681 agactttaga gatgttaaag gataatcttg aagatttaca ttctgtttct aaattcttat
     1741 ttgaaaaaga aactatgact catgaagagt taaaagattt gataggaaaa gaagctgtaa
     1801 actaatatag ttttaaaaat ttgaggctgt ctcaaaatag agataagcct ctttttattg
     1861 ttaaagttat aaaaatacat ttgattttat acaaaaaagt gcctcataaa ctaaaaattt
     1921 cattttgaga cacttacata ttttaattac atattttaat tttgtctatt tacagaatat
     1981 tatatctaca taaaactaaa ctgctgtaaa cttaactacc tccagtaatg tttgaagtgg
     2041 tataaaagct agtagcctag tttctacact tataatttat attttacata actaaattta
     2101 gtagattttt atactcacaa tttagaacga ttgaaaactt aaaaatttct tatcagatat
     2161 ctacaactta attaggtatc tatcacttga ttaaatatct atcacttaat taggtatcta
     2221 tcacttgatt aaatatctgc catttaatta aatatctacc acttaattaa atatctacca
     2281 cttaaagtgt agaacctctt tcaatcaatt caggttttaa catttcaacg actgactctg
     2341 gattttcttc atttaataag gacttaagta tgttaattgt tttttcaatc aacaaatcta
     2401 gtggctgtct aatcgtcgtt aaattataac tcttccatga agccatttga atatcatcat
     2461 atcctatcac tgataaatcc tctggcaccc tcaacttgta ctcaaacctt gcagcatcca
     2521 ttacaccaat agctatcaag tcacttgtgc aaaaaattgc tgttggagga tttgataatg
     2581 ctaacattct cttacctgct tcatatccag aatcataatc atagtttgca gattcttctt
     2641 taaggttatg aatcccactt tgtcttaact tagagtaaaa tccaattttc ttttccattt
     2701 cttcacttgt ttctctcgta aattgaatat atcctatatt cttatgacct ttcttaagca
     2761 gatattcagc agccattccc ccaccttcaa ttgggtctac atagactgta cttattttta
     2821 taccaggtat aaaacgatta aaaagaatca caggaatatt gttttcttca caagtctcag
     2881 ccatcacctt attcatggcc gaagctgtaa taatcacagc ttctacttga aactgaatta
     2941 cttttgaaat tatactatct atactttgtt gtctaggaac tttaaaaacg agacactgtt
     3001 tacctatctc ttgaattttt tctacaaata aattgataat tctattgtaa aatggaccta
     3061 gactatcacc aactatcaat cctataacat tagtttttcc actaatcatt ccccgtgcta
     3121 ccaaattagg aacatatcct atttcatttg ctacacgaaa aattctttct cttacttcag
     3181 gttttacgcc ctttccattt gcactaccaa atgctcttga aacagatgat tgagatactc
     3241 ctgccaatct tgcaacatct tttgaggtag ccgatttatt ataatgaact ttcttttgat
     3301 tcatttagta tttctcctta aatatctagt atttcttctt aaaattgtat caaaaataat
     3361 ataatatatt ggataataaa aaaagctaat acaattaatt cttgtttacc taaagaaaat
     3421 ggcactatag atactccatc tactttatcc attccttgag aaataaggtg atttctcata
     3481 ccaaatgcag tgtatttttt aatatcatct gcattatgat ttttctttaa aatgacacta
     3541 aaaatatatc caaatatcaa tgttattgta gcacctattg ctggattcca caaccaactc
     3601 acattatatt tattagaaat aaaaaatcct aaaactaacc ctacaataaa accaatagat
     3661 actcctttat cattagcttt atatgtaaac attgctagta caaatgcacc tgtagctggt
     3721 ccagcaaagt atgagatata gttaccaaca aggtcaagaa ttgatttgat gcttcctcca
     3781 aatcccataa taataaaacc tgtcataaca actcctataa ctacagttat aatcattgta
     3841 acttttaatg atgattcttt gttatctttt ttgaaataaa actcataaat atccttcgta
     3901 aaaacagttg tcatactgtt taatatagag tcgatactag acattgctgc tgcaaacaca
     3961 cctgcaataa taattccaat ggctcctact ggaagttcat ttaaaataaa agtaatcatt
     4021 atttcatttg aagtttcaaa tgtccttccc ccataaaaaa caaataaaat tactcctacc
     4081 aatagcataa agtagtaaac taaattcata acaaatgcac taactacaaa agaatttttt
     4141 gcacatttta aagatttaga tgttaaaact ctttgtactt gagattggtc aaaacaaaag
     4201 tatcttatcc acattacagt tccaccaatt aaagtagccc aaaaagtatt tgtcactgaa
     4261 atatccctac tgaaatcaaa tgtatttaat tttccactct gttgtgcaat ttgtaatgta
     4321 tcaaagaaac ttagattcat tccttttaag gctacaataa ttactataaa tacagcactt
     4381 attactacta tcatttgaat actatcagtc catataactg ccttaatccc acctaataga
     4441 gtgtatataa ttgatacaag tacaactatt gggactaaag cataaagtga ccagccagta
     4501 atcatttgaa taataagcac tggaatatat accatagaac tagcttgaat caatgaatta
     4561 ataaaaaatt gaataatagt taatcctctt gaataatttc ctaaacgaag tcccatatat
     4621 tcatatatag aggttatttt taatttatag ataactgggg tggtaatgct cagtgcaaaa
     4681 aatattgcta aaggtactgt aatattaacc attactggat acattccatc tacatatgcc
     4741 catccaggac cacctataaa gccatttgca ctaatcatag tagctgcaac tgataaagca
     4801 attggaatcc atggcattga cctacctgcc aagaaataat cttcttgatt tttattatct
     4861 tttcctgaat aataaccaat aactaacatt aatagcaaat atcctattat aatacataca
     4921 tcaattgatg aaatcataag ccaccccttt ctaagtaaca aaataaattc ctagtatttt
     4981 attttttatt atttaacgtc tctggtaaga ataaccatac aatagcacct gctacaaaga
     5041 ctgcgattga tatgtaaaag atagtcatca tacctaaagt accagaagct gtaccagcta
     5101 tagcaggtat tgcaaacgta gatataatcc ttcctccatt aaatgacata ccagatgaca
     5161 acgaacgata tttacttggg aacaactctg tgtagtatgc tccccatgct ccagaatatc
     5221 caacagcaac acctagtaat actgatatca tatagaatgt tgttgtatta tctccaccta
     5281 attttgtata tataaaaaca aatactgctg ctaatgccat acctataaaa ggtatttttc
     5341 ttccaaattt atctgcaatt gaaccccaga ataaatatcc tactaattga ccaattcctt
     5401 gtaaactata tatagactca gccactgctg ctgacattcc tattcctgtc actaggtatg
     5461 tagttgcatt atttgaaaat gctgaatatg ctgtaaaatt tgcacctgat aaaagaatcg
     5521 ttcccaatcc aatccataag tattttccct tatacatact tatatagcta atcttttcac
     5581 cagtttcttt tcccttttta actaattcct ttgtatactc caaatcatct ggcacaaaga
     5641 agattgctaa gatagctcca actataggtg gtataatcgc agtatacatc aatatttgcc
     5701 aatttgcatc acctaaaacg ccatatactt tcccaccaaa aatcattgct aatgaaaata
     5761 aagcagttaa tatagcagct aacttaccac gatatttact tggaaacata tctgcaacta
     5821 aaggcatagc tgttccaaat acgcctccca acactactcc tgtacacaaa cgtgctattt
     5881 tccataacat aatatttgta ggagataaca ttggtataaa tgttgtcaat ccaatcagta
     5941 aagcaacgct aattaaagtc ctcttcttcc caatttttgt agaaatcacc ccaaaaataa
     6001 atgtacctgg aattgtacca aaagacataa ctgcaaatat ttcttctgtt tgttttaatg
     6061 aaaaaccaaa tcccccatct gctaaagcat aaatcattgt tggtttaata aaagtaccca
     6121 ccataaaatt gtagcaataa aaaaagtata ttaaagcaat tgaaaaaaat gcaattcctc
     6181 tttttaggtt tgaatagtct tttcctaata tgatatcatt ttctatttca accgttgttt
     6241 tcatattttt ataattcccc cttttaaatt tagtcataat tttgctaaaa gtttaaacat
     6301 ctatttttca tatacattcc ccacttaaat tttataaagc aatacttccc tatatatttc
     6361 aaaactattt atactctaaa cagctttatt cttctactcc catataaatt tgttttaata
     6421 catccatacc atcaaaagtt atccactcct cctttacttt accttgtagt atatgataat
     6481 gattgattcc cattacttct atatgctttc cagaaggctg tccaaaaaat cctatacctt
     6541 catttattcc acgtaatctc catcttacag atacatcata accttcattt ttttctcttt
     6601 gattacaagt tactctttcc acttcataac ttccatttgg aattgatgca aataaactta
     6661 caatcattcc ttgaatctca tcatagccat ttaaatcttt atcacatata aaatgtacta
     6721 ctgcattatc atgatagtat tttttaactt catttatata tttataatta taaatacggc
     6781 ttgtcatttc aagcatcatt tcgccaacag aatcatcttt tgcttgatac ttagttggca
     6841 taaattgacc atccatagat tcacagatac cataagttga ttgtaaacta agcactttag
     6901 attcacttgc ctttgccata ctctttgcta cttcctgagg gtttaatcct aattgagtaa
     6961 caatccataa attatctcta accaaccatt cttcatggat tctattgtta gttgtggcac
     7021 aatcaattac agttctaaag tttatttttc tacctgttgc tggtccaaaa ttactatcac
     7081 ctaagttagt tgctgtagac aaaacacggt gagaagataa aaaaccatca gcaccaaagt
     7141 tagaccatat tacattttga ccaattaatc ttctatcagg aaacgcatgt aatgtttgaa
     7201 gagtattgga aataacatct ttaataccaa ctaaatttga agagccacaa tgcattgtaa
     7261 cattattgtg atatgtatca taaataacac ctataccttt ttcttcccag atattatgtg
     7321 taatcttcat aatataatct acaaaatctc tatacttaga atcaaatcca ggtatttcct
     7381 gttccctttt tgtctgtttt gaaaaatcat tgtaatccat tactccaaca ggcgttactt
     7441 ctaatgaatc tccatagtaa atttttgtag atgacatatt atcttccccc ttaaatataa
     7501 cttttttatt tttttcagta agagtatttg tatttaattt atctgtatcc actgtatcta
     7561 tgttaacttt atttatgtcg gatttatttg tgtttgtttt atctatattc cctttatcta
     7621 tatttccttt atctatattt cttttatatg tgttcgtttt attctcattt gtttgatttt
     7681 catttatatt actaaaatta gtttgattca ttttagcttt gttttcttta accgtacatg
     7741 attcaagccc acttgtagaa acttcttttt cttcctcttt acttgttgca ttatttttgt
     7801 tggtagatgc cactttatat ccccctttgt tttagaatac gtatgcataa ctaagcatta
     7861 aaaatacttt tagttctctc aagaatatca agcccttgtt gtttcaacca ataaggaata
     7921 tcaattaaca cccagttttc ttgaagttta tcgcccttac gataataaac atcaacaact
     7981 tgcatatctg ctttaacctc tccacctggt aatcctaaaa aacctccaca tggtacatta
     8041 gtcagattag gccatccaaa gaaacaagca aaattacctt ctgcaaatct acatacatgc
     8101 ccattaaaag tctttccttt taaattattt ctaaatggta gctggtgttg ctcttgatat
     8161 ctaggtatag tataagaagc accaattcca cctggaccat accaaatcat atctttagcc
     8221 caagattttt caagtatttc tggtgggcat cccatagcac cactttcatt tagttcagat
     8281 aaatcatcta ccattttatt tactaatgct aaagttttta ctccttcaac ttcatctgca
     8341 tcttcaaaaa gtaatccatt atggtcaatt ggccctggat atacaaaata tgaacctgtt
     8401 tgtggtggca atggattaac tcctgcttga atcataaacc caattatatc tacaaataat
     8461 ccagttttag caattttacc atcttgaaca cagttaaatt ctgcatatct taaactaatc
     8521 attttacgag tatgtttaat gccaagccaa tcgttatcaa aaagtcccat aaaatgcccc
     8581 atactcatta cccattcact attgtcaatc tcatttgttc ctgcaatgaa aatatcttgt
     8641 cgtctttgca tattactcaa tgattttttt aaaggcttcc aaaatacatc tgctacattt
     8701 tcagtgcctt cttgttcacg aaatggatac acacctttcc agttataatc tgatttcata
     8761 tgttgcttta agacattttc aacctcatcc ccagtagcat tttctaatgc ttcaaaataa
     8821 tttcttacaa ctttttttga atcctgatat tttcccataa ttatctcctc ccagatttat
     8881 ttaagttata aattaacttt ctcatttcat gcatacgatt tcctttttaa tgatttttta
     8941 gctgttttaa gtttatttta tgaatacata tgcatttttc taaatattaa catatatcta
     9001 aaaaattgta aagaaaatgt tatcataaaa atttaatttt tgacttatat ctatttaaaa
     9061 caacagaaaa ggtttaaaac gtaatattct actgggtcta ccatacttgc tcataagctg
     9121 ttcactcact acctcacaat aaccagaatc acacaatcta agtataatcc tgtccatagt
     9181 tctaatactt acaccacata tagtagctaa ttcctttgat gtaaattcag tttttccact
     9241 atttattatt ttattgaata ctttatatac agtattagca cttagccctg tctctgtagc
     9301 aatttgataa aaacgttcat caaaactatt tttctctttg acagtaggat ttggctttag
     9361 tggtcccata atttctccat tttcaaaaac tacatatgct gcatcatttt tttgactttg
     9421 agccatctta atactctcat aagcgttaaa tttagcttca tttgctgttt ttccataacc
     9481 aattcctata taagtgttta taactgaaac ttcacgaatc atatccaaca aataaatatc
     9541 tctgtatttc tttgtttcta actcaagtat ttttttagtt gtaaaaatca taaattcact
     9601 tctactctgc tctaccaaaa caccatctat acggctattg aaatggtata gtttatcaag
     9661 aatcttaatc ctttgtgaaa gataagcgta ctcatcttcc ttcattagtg aatattctga
     9721 aggcatgttc agtttaatag caagaacaat aatctgattc tcattgtttt tctttgcaat
     9781 ataacgcatc tgaatgtttt ttatactttg caccattacg tcctcagaag gcaaggctaa
     9841 aactactgga atattatctt ttttcattat atgataggcc tcttcaagac ctgtaataca
     9901 acaacataca tcattatttt gataattata tttatgaaat tcaagcacaa actttatata
     9961 gtcctcttct aaatactttt gctctgcaaa aaacatatgt tcatccttaa attcaactcc
    10021 aatttcttta tacaatctct tcatcatagc cttatcataa gtatctatac taagtctact
    10081 aatatcgaat ttaagcaaat atgtaatttc aagcaacact ctgtacaatg ttgtttcatg
    10141 tctaggaata taatcagaca atacaaaatc tttttgtctt ttttcaaaaa atttaaatgg
    10201 cgacttacct gtaaaaagaa ctgcatccac tctacctcta tatttttcca ataaatcagg
    10261 tacttcaata tatctatcat atctaagaaa atctgcttca atattaggaa aattcttttt
    10321 acttactgct agtattttat cgattaccat acctggtcct acataaagta ttttcatttc
    10381 gtctctcctt tcctaatgat actattatct cttaaaacat tttctaaacc taccatgact
    10441 aaagccacag gactcctgat tcatagaatt ttgaataatc aattatacct aatataaaaa
    10501 gttactttaa gttttttaat tacgactatc tgttgaaaca tttttgtatt taaatttttg
    10561 tatttaaatc ttattcatta atattaatta cattttacaa ttaatatatt gttttttcta
    10621 cttaaagtta aattctttag aaaacacaag tagagtaatc tttattaatt taacaaaaat
    10681 agccttcctt aatattcaaa gaaagactat ttttataaaa gttcttaatg tattctattt
    10741 tgctatgcta agaggatttt taattacacg tccagcacat acatcagttt gctttcctcc
    10801 tgccaatgca attttaccat tcaccaacac atattccact cccactgtat actgtttagg
    10861 attactataa gtcgctttat cttctattgt atctgggtca aataccacaa tgtcagcaaa
    10921 ataattttct ttaagtagac ccctttcttt ataaatgcct aagcgttttg ctgaaagata
    10981 agttatcttt ttaactgctt cctcaaaagt caacatacct ttttctctaa catagtaaga
    11041 gaaaattcgt ccaaaagtac caaatgcacg aggatgaggc tttccaagag aaagtaaacc
    11101 ttctgtagaa aggtccatac tatcagaacc tatacatgac tttggatggc aaacaacctt
    11161 atctgtatcc tcttgacctt gtgtatgaac aactacttga attttacttt tttcttcaat
    11221 caaaagccta aagaacgtat caaatggatt ttcaccattg attttggcta cctccattaa
    11281 agttttaccc tctacatatt tatttgcttc agtcattaca gaggctagta caacaccgtt
    11341 ccacccacct gctaattggt agaaacattg ccatccagga atcccttcct ccaactcttt
    11401 tataattctg tctctatttt cttgtacttc taatcgtttt accatatttt ctacaccgcc
    11461 ttcaaaagcc caaggaggta aaagtgcacc caaagatgtc attccagctg tatatgggta
    11521 acaatcaaaa gttacttcca aaccatctgc ctcagcttca tctagctttt taaatacctc
    11581 atcaatagtt ccaaaattct tatgacccat aacttttaaa tgagaaatat gtagaggaac
    11641 tcctgcagtc ttagctatat aaattgcttc atcaagagct tccatttctt catcaccttc
    11701 atctctaata tgagatatat agtatccatt gtatggtatc aattccttac ataattcagc
    11761 catttcttct ttctttgtgt atgctcctgg taaataaact aatcctgatg aaagagctac
    11821 acatccagct tccatagctt ttctaacttc caacttcata tttttaagtt gctctaaagt
    11881 agctggttcc tttgaaaatc ccatttcatt gatacgtaca ggtccttgtg ctacataagc
    11941 tacaacattg actgctggag aattttcagt taagtaatca aagtattctt gcattgtatt
    12001 ccaatgaagc tctaattttg ttggaattgt accaattatc ctagttccaa gataatcaat
    12061 taactcttgc ttcttctctt ctcttatcgg agcaactcct ataccacagt ttgcaataac
    12121 atctgttgtt acacctgtat ggatacggct tcttgtgtat ttatcataca caagagataa
    12181 atcagaatga gtatggaaat ctataaaacc aggtgataca aattttccag ttgcgtctat
    12241 agtctcttta gcctcttttt gacttaaatc tccaatagcc atgattttgt ctcctacaat
    12301 ccctacatca cttttatatc ttgaatttcc tgtaccatca acgattattc cattgacaat
    12361 tttaacatca aacatgcttt ttctcctttc aagacttatt tatattcata tttcttaata
    12421 aacaatccta ttcttttttc tcaacaaata attctagtct ttctccttaa taaacaatcc
    12481 tattcttttt ctcaacaaat aattctagtc tttctcctta ataagcaatc ctattctttt
    12541 gttttattgt tatcaattac aaagaagacc cttgtgataa gtatcccacc taacacaata
    12601 aacaatgtta ataatccacc tggtatacca attttttcac caaagtacat aattgctaac
    12661 gctacagcaa tcgtacatcc accagctcga atatccttac gtgcaatatc aacataaatt
    12721 gctccaaata atgctggaag tatataatta aatgaattag taacaaattc aggtaacaaa
    12781 ggaattactt tatagcctac aatggtaaaa atggtaatca tagaagctga tacaaagatt
    12841 gaacaacatg taccaattgt tccaacaact tcaccctcat gagttcctgc ttctacacct
    12901 gcaattttct gtgccattga tgctgcaggc atacgtatat ctccaacaga acctgcaagc
    12961 caacctatat aagaacctgg agctcctagt gtaggataat aagctatagg ttgaacaatc
    13021 caactaaccc cataagtcgc agcgactaac ccccaaattt gaaatataac agaaagcatt
    13081 ggcatctgac caaagcgaat acctacatag atagcgggaa taaagtttgc aataattgca
    13141 agacctatag aaattctccc tatttttatc atctttttat catacataat tatcacctct
    13201 tatccgtttt aatcacgata atataatctg tgtaccaaac ataccaatta acatggcaat
    13261 acctaaggat aattcttgta atcgttgatt ctttttaaat aaagttgtca atactagcat
    13321 aactatagct gaaataattg ctggtgtaag ttttggtatt tcaaatgttt ttatttgatt
    13381 tgaaagaagg taagcaaata atccaactgt agcacctgca agtagttttt taacccaagt
    13441 aggattatac ttcatgttca tcttttcaac aattccacgc attctatgac ccaaaataaa
    13501 tacaacaaat agccaaccta agttatttaa agccatcccc catagtgcat acgaaaaagc
    13561 ttctgctcca aatgcagcac tacctacttc taccccagat aaattggatg taagagatat
    13621 tactccaagc tctgttctag ctgctccgac atcacagagc ctcatccaag tcgtaggagc
    13681 gccaatcaca gctaccagtg ataataatgt aataacagga gatagagaag gtccaatggc
    13741 tgtaacacta gctgagcgaa ttgctgcttt aattcctttc ttttcaatac caagatttga
    13801 agcctctttt agtgcctctt tcataaatac gattgcttga acaactgaaa taattactaa
    13861 aaaactagaa acaatccaaa gacctgggct attaatcatt tcttgaaact tcatacttta
    13921 ttcctcctta aaataaattt ttcagcattt tattgtgagc tttctttctg taatcttgtt
    13981 atgtttttat attgttttaa tttattttat ctaagatata gtatctttaa taggagatta
    14041 tctgtcttaa agtctcacta aaaacattaa tacttaaata ttttattcat aattagtaaa
    14101 tatcagaaaa ttcacatatt atttttttaa cctgtaatta
//
LOCUS       DAYAPA010000116        13932 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.118, whole genome shotgun sequence.
ACCESSION   DAYAPA010000116 DAYAPA010000000
VERSION     DAYAPA010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13932
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.118"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            123..485
                     /locus_tag="ACGI59_003036"
     CDS             123..485
                     /locus_tag="ACGI59_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HFG9740814.1"
                     /translation="MIKLYGYTKCSTVKKAKNWLKENNLEFEDIDMVQNPPSKEELKS
                     IYKTSGYDIKKFFNTSGMKYRELGLKDIVKTESDDKLLEILVSDGMLIKRPLLLDGKN
                     VLLGFKEDVWKSTLLKED"
     gene            921..1622
                     /locus_tag="ACGI59_003037"
     CDS             921..1622
                     /locus_tag="ACGI59_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4253 domain-containing protein"
                     /protein_id="HFG9740815.1"
                     /translation="MSIEDLYDFECVPLNNEIDIDVLFEKMIEDGKEKEYTPIIVIDE
                     KVGLLRKNIDMITKEYGSLENYREYCLTKYNEIDVNQFFDFRKSEISELLKELIDLED
                     DFYDYKHFRPIMNIDIFHEYNKVYIAKIPTIKPYEVFAYIPIGGFNDCPRDEEHIAIA
                     KYWYEKHGIFPIAIGCDTVQYSVKNLTKDGKKFDELCIELVFYCEDIIIQGYETLKAL
                     KDVLKRSTIVLFWWD"
     gene            1893..2711
                     /gene="larE"
                     /locus_tag="ACGI59_003038"
     CDS             1893..2711
                     /gene="larE"
                     /locus_tag="ACGI59_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HFG9740816.1"
                     /translation="MEVNFVKEKEKLDKLKKMLLELGSVVVAYSGGVDSNFLLKVAKD
                     TLGENVIAVTIHAMMHSSREIEEAKQYTQNFGVKHIILNIENFDLKEFKENGVDRCYH
                     CKKYIFSKIKEVAKEHNIKYIVDGTNIDDLGDYRPGLKALSELGVISPLKDSSLKKEE
                     IRSLSKTLGLNTFNKPSFACLASRIPYGVEITDENLRIVEKSEEYLSNLGFSQFRVRM
                     HGDIARIEVGQEELGKFFENNNFNKVDTKLKIFGFKYVTLDMSGYKMGSMNLNV"
     gene            2766..3914
                     /locus_tag="ACGI59_003039"
     CDS             2766..3914
                     /locus_tag="ACGI59_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HFG9740817.1"
                     /translation="MKNYTLISPCFFGMEKMLAREITNLGYEIIKTEDGRITYKTDEF
                     GIAKSNMWLRCAERVHLKIAEFEAKSFDELFENTKRINWSRYIPYGAQFPISKASSIK
                     SKLYSTPDVQAIVKKAIVESLKKSYLEDGLLKEDKEKYPIFVFIHKDKVTISIDTTGD
                     ALHKRGYREKANKAPIRETLAAGLIYLTPWKAGRVLVDPMCGSGTILIEAAMIGINMA
                     PGLNREFISEKWRTLDKKIWWDVRKDAFNKIDNESKFKIYGYDIDEESIDIARENAEI
                     AGVDEYIEFNVGDATQFKSEDEFGFIITNPPYGERLEDKDSVKQLYKELGYAFRKLKN
                     WSYYLITSYEDFEYEFGQKADKKRKLYNGMLKTNFFQYPGPKPPRNNK"
     gene            4050..5438
                     /locus_tag="ACGI59_003040"
     CDS             4050..5438
                     /locus_tag="ACGI59_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFG9740818.1"
                     /translation="MKVLKLITVVALMMLFVTGCNIKTESPEQLAKIPDYDDTKKVLY
                     DGIDQLLKPDSSMILPSNTKEVGKINKVDLNSDGIDELVVFEQKEDLSNNVSQVGFVT
                     LSYNGEKYVLGDHFLENGESIEYANFYDLDSDGYKEVILLVKSKDKTNMHIYKVVDNE
                     ITKIYDLDASWLQNKEDFNDMKVKIGYIDGDDKLDILMLHLNNKTNEMFASVANFDGK
                     MKIKDSVKFENVKNLSELYITLGNVASSVGNSSAAIKGIVLDIPILKDNNYITQILYM
                     KDGKINKAFSDYDKTITKSYYIPVDDIDKDKIIEIPIVAGSTGNNKNTYSSKSSATIS
                     WYRWNGKEGADSSLIFTSQIYYNYKYNFKLFIPNNLFDKILVEQEFVGEKAVFKFYYF
                     DYSDRKNLFNIVVESKNKLEDRKNTGTQNSIVLQESDEYTFLLVVNNANEMDKLDMTI
                     DALKEYFSLIYG"
     gene            5467..6165
                     /gene="walR"
                     /locus_tag="ACGI59_003041"
     CDS             5467..6165
                     /gene="walR"
                     /locus_tag="ACGI59_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism DNA-binding response
                     regulator WalR"
                     /protein_id="HFG9740819.1"
                     /translation="MKEKILILEDEIGIRSFVSINLKREGYEIVEAGTGREAIEKMTT
                     EKDITIALLDVMLPDISGIEVCKFIRENFDQVGIIMLTAKAQEDDKIEGFISGADDYI
                     IKPFSIKELLVRVSALLRRVAKDDSSVKSSEIVSPPFILDIDKRKLFKNGKEIELTPT
                     EFSIVKYLISNAKQSLSRDQILDEVWGTNYLYDFKIVDVNIRRIRNKIEDDPSKPKYI
                     QTIWGYGYCFRKEE"
     gene            6168..7601
                     /gene="walK"
                     /locus_tag="ACGI59_003042"
     CDS             6168..7601
                     /gene="walK"
                     /locus_tag="ACGI59_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HFG9740820.1"
                     /translation="MLHFEGLRKKVIKNYFIIIIIMVTLFEGLFMFYIQNYYYDSVKQ
                     LLESEIKYADEYNAITMETTSFEKKVKNIFDKQPLTKNSEFGISIIDKDKNIILDQYG
                     FKSKEKANYEDVNNALKDIKTKNLTPYTYRIPDTGEHVMSISLPLKVNNIIEGVVRYT
                     VSLDAIDNAILKQATWLILAGIFILIIAILISLKFAETLIKPLRELKKFANELAVGNY
                     NIKLEKMKIVDDEIGDLAQTFEHMAHEIDKSEKLKEEFISSVSHELRTPLTSIKGWSE
                     TLGYESITREELDLGLGIIQDETERLIKLVEELLDFSRLSSDRIKLHVDIVDVEGLIV
                     GVVNQLKVKAAEKDISLLFEFENEFIENIQGDKNRLRQVLINLIQNSFKFTSQGGYIK
                     VVASQDEEITTISVEDNGSGIEKQNLNKVLDKFFQEDYNKAGSGLGLAISNEIVKLHG
                     GRMKIESEKNVGTKITFNIKNKFAKQA"
     gene            7623..8360
                     /gene="truA"
                     /locus_tag="ACGI59_003043"
     CDS             7623..8360
                     /gene="truA"
                     /locus_tag="ACGI59_003043"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFG9740821.1"
                     /translation="MRNIKMIVAYDGSRYKGYQKLGDNNMTIQEKLENVLSKMTNETV
                     EIIGSGRTDMGAHARGQVVNFRTNCMDSLDKIQKYLYEYLPEDIVVKTVEEVDERFHS
                     RYNVKSKTYMYKIDNNKYHSPFIRKYATHVSKKLDLDRMRKASEYLVGEHDFTSFASS
                     KSKKKSNVREIYSINIKEDDNVIEIYVEGNGFLYNMVRIIVGALIDVGLKRRAPQDIK
                     YMLESKDRCQSSDTAPAKGLCLWKVTY"
     misc_binding    8471..8678
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            8931..10130
                     /locus_tag="ACGI59_003044"
     CDS             8931..10130
                     /locus_tag="ACGI59_003044"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HFG9740822.1"
                     /translation="MKEKVVLAYSGGLDTSIIIPWLKENYEDIDVIAVCGNVGQEDKM
                     EDVYEKALQSGASKAYVDDISEEFVTETIFKAVKAEAKYEGKYLLGTSLARPIIAKKL
                     VEVAHKEGAKYICHGCTGKGNDQVRFEATIAALDPTIKVIAPWRIWDIKSREDAIDYA
                     EKHNIKVTATKAKIYSVDANLWHVSTEGGDIEHLENEHKKDVYKQCVDPEDACDVAEY
                     VEVYFEKGVPKKVNGEELSPVALIHKLNELGCKHGIGVIDIVENRLVGMKSRGIYETP
                     GGTILYEAHNILESATLDKDTLHFKQMVSYKYGELIYNGLWYCKLRESIDAFMEQTQD
                     NVTGTVKVKLYKGNIKPAGIFTENALYDEGISSFGNSELYDHKDAEGFINLFTLPLKI
                     RAMKAGK"
     gene            10377..11423
                     /locus_tag="ACGI59_003045"
     CDS             10377..11423
                     /locus_tag="ACGI59_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtrH N-terminal domain-containing protein"
                     /protein_id="HFG9740823.1"
                     /translation="MRKIIEIKHHSHDYECMWNGIEDLYINKTGECLPNDFFFLLSGF
                     GSFCYMKTNKADLKRMVALGDGRTKQMYKFLAPIVGFEYKHHEFKKYEQALKKAKSEI
                     DDGYPVILGALDMYYLPYYKKLYHKEHIPFHYILMVGYDDEGIYLYDCGRTELLHLSY
                     DKLRESMNCNSPGLSKENTICTIRMNTSKNKYNIAREAIAIKRDMFLNPPKGFLGYKG
                     FEKFIKELPQWNTELDKDDYDKILTNMVMFFGTVPTIPNAIKGIKEPDTVQFKGGFDK
                     MSRMLNSIGEEYKNDSFIQVADIFDKTAIIIEKISNIIIDYLTQTCDDTGQLPLLFSE
                     VLEHMKTGYLILDV"
     gene            11531..12694
                     /locus_tag="ACGI59_003046"
     CDS             11531..12694
                     /locus_tag="ACGI59_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFG9740824.1"
                     /translation="MRTVKQVSDLTGISVRALHYYDEIGLLKPNKITDAGYRLYDDES
                     IKTLQQILFFKEIDIPLREVKEIMSSQYFDKVEALKNQKKLLILKRKRLDELIELINQ
                     TLRGEGNIDFKEFDMSEYFDVLEEFKREHRNKVIKIYGSVEKYNEYIERVKSNEEKIA
                     KMAIKQYGTIEKFAKAIKTNFSSDILNLGEKFDRYKNDCLKEKHPKLKELYRKLVEDL
                     NRNPSSTDLQEIAKEITDISKKDYEIFSMDTGDDNWYYMVQNYLVNPMWIEEVDKKYG
                     SGAGKFIGQVLKTYLRDRKPKINTLYEKLVEDLSRDCSSREVQSIVEEIDNEMKRSNE
                     FYKIDNGERYFDYMSELYLQDSNYIKVTDKNYGDGASKFIGEAFKIYFDNNNC"
     gene            complement(12783..13163)
                     /locus_tag="ACGI59_003047"
     CDS             complement(12783..13163)
                     /locus_tag="ACGI59_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4363 family protein"
                     /protein_id="HFG9740825.1"
                     /translation="MKSTTFVILWTILFVLFGFYVNNKLYDFTEGYKDNISVLEKSIE
                     NEDWEKAQKEADSISTSWNKEKNHWYKVLNHEYFDEIGLRFNILDKAIYTENKLKSLE
                     EVESIKTYLGNIIESVKFDVNYIF"
     gene            complement(13166..13825)
                     /locus_tag="ACGI59_003048"
     CDS             complement(13166..13825)
                     /locus_tag="ACGI59_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFG9740826.1"
                     /translation="MTVVLIRSIILYITVLIALRVMGKGEIAEMNCFDLVITLLIAEV
                     ASVPMENNNIPIINGVAAISGLVIMQTLISFLSLKSRKLSSFLSGKPSVLIDKGKIVY
                     KELKKERISIDELLEQLRIQGYFNLKDVQYAILETDGNLSVVPASSYNSTPPRAFNHL
                     PIPLILDGRIINKNLDIAQKDTNWLMGILKSNHIETFKDVLICVLDENDKIFIQNKKG
                     D"
BASE COUNT         5623 a         1359 c         2380 g         4570 t
ORIGIN      
        1 tttaataatt tgaaatatga taatttttta tgttgaaagc ttataatgat tatgatataa
       61 attggttata gtatacaatt tatgggtata acaataaaaa attagaaagg aatttttata
      121 aaatgattaa attatatgga tatacaaaat gctctactgt taaaaaagct aaaaattggc
      181 ttaaggaaaa taatttagaa tttgaagata tagatatggt tcaaaaccct cctagtaaag
      241 aggagttaaa atcaatttat aagactagtg gatatgatat aaagaaattt tttaatacaa
      301 gtggtatgaa atatagagaa ttgggtctta aagatattgt aaaaactgaa tctgatgata
      361 aacttttaga aatattggta agtgatggta tgcttattaa aagaccatta cttttggatg
      421 gtaaaaatgt attattgggc tttaaagaag atgtatggaa aagtactttg ctaaaagaag
      481 attaggaaga gataggtgtg ttgattaaaa ttattaatta atacacctat aaatttatgt
      541 attaaatcta atttttataa atattaatta gcaaattcaa atcaaaaaaa ttcatatcaa
      601 caaacatatt acttcttatt acaaattata tcatatgttt tatattaaaa tattataata
      661 ttacaacgaa ttgttacaaa attacttatt attaaccatg aacatttgtt ttgtgctatc
      721 atatttatat aagggaattg tcataaaaaa tatcttgttc atacagtttt atatagaaaa
      781 aagaaatgaa tcttttcttt atataagtat attttgtaaa ttgcctctat atttatagtt
      841 taaataatag tggtattaaa aatctgacaa caaaaataaa actttcaaaa aattaaatct
      901 aaaaagaata ggagataaat atgagtattg aagatttata tgattttgaa tgtgttcctt
      961 tgaacaatga gattgatatt gatgttttat ttgaaaagat gatagaagat ggaaaagaaa
     1021 aagaatatac accaattata gtcattgatg aaaaagtagg gctattaaga aaaaatatag
     1081 atatgattac taaagagtat ggttctcttg aaaactatag agagtattgt ttaacaaaat
     1141 ataatgaaat tgatgtaaat caattttttg attttaggaa gagtgagatt tcagaattat
     1201 taaaggagct aattgattta gaagatgatt tttatgatta taaacatttt agaccaatta
     1261 tgaatataga tatatttcat gaatataaca aagtttacat agctaaaatt ccaacaatta
     1321 aaccatatga agtatttgca tacataccta ttggcggatt taatgattgt cctagagatg
     1381 aagaacatat agctattgca aaatattggt atgaaaagca tggaatcttt ccaatcgcaa
     1441 ttggatgtga tacagttcaa tattctgtaa aaaatttaac taaagatggt aaaaaatttg
     1501 atgaattatg tattgaatta gtattttatt gtgaggatat tattatacaa gggtatgaaa
     1561 ccttaaaggc tcttaaggat gtattaaaac gctctacaat agtgcttttt tggtgggatt
     1621 gatattccat catttagata gtattaaatt gttagtcacc aagaatccac ttgctttagt
     1681 tatggggagt gtgtcaaact aggtttttat acacagtgta gcatagtaat atgtttaaat
     1741 tacaaaggtt aaaaaaatac tttaatattt ttaatattat aattaataga tttaaaggta
     1801 attataattt ataaaataaa tactttatat gttataatca ttaagaatgt aaaaaaataa
     1861 tatatagttt tatagttcat atttttccag atatggaggt aaattttgtg aaggaaaaag
     1921 aaaaattaga taaattaaaa aaaatgttac ttgaattagg cagtgttgta gttgcttatt
     1981 ctggtggagt agatagtaat tttttgctta aggtcgcaaa agatacatta ggtgaaaatg
     2041 ttatagctgt gactattcat gcaatgatgc attcaagtag ggaaattgaa gaggctaaac
     2101 aatatactca aaattttggt gttaaacata tcatattaaa tatagaaaac tttgatttaa
     2161 aagaatttaa agaaaatggt gttgatagat gttatcattg taaaaaatat atattctcta
     2221 aaataaaaga ggtagcaaaa gaacataata taaagtacat agtagatgga acaaatatag
     2281 atgatttagg agactataga cctgggctta aggcattgag tgaattagga gtaattagtc
     2341 cattaaaaga tagtagttta aaaaaagaag aaattaggtc gctttcaaaa acattaggat
     2401 taaatacttt taataaacca tcatttgcat gtcttgcgag taggatacct tatggagttg
     2461 aaataacaga tgaaaattta agaatagtag aaaagagtga agaatatcta tccaatttag
     2521 gtttttctca atttagagtg agaatgcatg gagatatagc tagaatagaa gttggacagg
     2581 aagaactagg taaatttttt gaaaataaca attttaataa agttgatact aaattaaaaa
     2641 tatttggctt taaatatgtt acactagata tgtcaggata taaaatggga agtatgaatt
     2701 taaatgtgta gattatttta aaaataaata agttttatag ttaatataag gaaacaggag
     2761 gttttatgaa aaattatacg ttaatatcgc catgtttttt tggaatggaa aaaatgctgg
     2821 ctagagagat tactaattta ggatatgaaa taataaaaac agaagatgga agaataacat
     2881 ataaaactga tgagtttgga atagcaaagt caaacatgtg gttgagatgt gctgaaagag
     2941 tacatcttaa aattgctgaa tttgaggcaa aaagctttga tgaattattt gaaaatacaa
     3001 aaagaataaa ctggtctaga tatatacctt atggagctca atttcctata tcaaaagctt
     3061 cctctataaa atcaaaatta tacagtacac cagatgtaca agccatagta aaaaaggcta
     3121 tagtagaaag cttaaagaaa agttatttgg aggatggttt gcttaaagaa gataaggaga
     3181 aataccctat ttttgtattt atacacaaag ataaagtaac catttctata gatacaacag
     3241 gagatgcttt gcataagaga ggatatagag aaaaggcaaa taaagctcca ataagagaga
     3301 ctttagctgc tgggcttatt tatttaactc cctggaaagc aggtagagtt ttagtagacc
     3361 ctatgtgtgg ttctggtact atattgattg aagctgctat gattggtata aatatggcac
     3421 ctggcttaaa tagagagttt atatcagaaa agtggagaac tcttgataag aaaatatggt
     3481 gggatgtaag aaaagatgct tttaataaga tagataatga atctaaattt aagatttatg
     3541 gatatgatat agatgaagaa tctatagata tagctagaga aaatgctgaa atagcaggtg
     3601 tagatgaata tatagagttt aatgtaggag atgcaactca atttaaaagt gaagatgagt
     3661 ttggatttat aatcactaat cctccttatg gagaaagatt agaagataaa gacagtgtta
     3721 aacagttgta taaggagctt ggttatgcat ttagaaaatt aaaaaattgg tcgtattatt
     3781 tgataacttc ttatgaagac tttgaatatg agtttgggca aaaagcagat aaaaaaagaa
     3841 aattgtataa tggtatgtta aaaactaact ttttccaata tccgggtcct aagcctccta
     3901 gaaataataa ataagtttta catgatttat ttgtaaaata taaaatttta ttaaaataag
     3961 taaaacatta tgatgccttg aaacgtctaa ttattgtgag gagatttttg tgaacaaatt
     4021 taaagattta taggattagg aggagttaaa tgaaagtgtt aaagcttatt actgtagtag
     4081 ccctgatgat gttatttgtt actggatgta atataaaaac tgaatctcca gaacaattag
     4141 cgaagatacc tgactatgat gatacaaaaa aagtattgta tgatggtatt gaccagttac
     4201 taaaaccaga ttcatctatg atattgcctt ctaatacaaa agaagtagga aaaataaata
     4261 aggtagattt aaacagcgat ggaattgatg aattagtagt atttgaacaa aaagaagatt
     4321 taagtaataa tgtgagtcag gtagggtttg taaccttgag ttacaatgga gaaaaatacg
     4381 tacttggaga ccatttttta gaaaatggcg aatcaataga atatgctaat ttttatgatt
     4441 tggactcaga tggatataag gaagttatct tattggttaa atccaaagac aaaacgaata
     4501 tgcatatata taaagtagta gataatgaaa taacaaaaat atatgacttg gatgcatcat
     4561 ggcttcaaaa taaagaagat tttaatgata tgaaagtaaa aattggatat atagatggag
     4621 atgacaagtt agacatattg atgcttcatt tgaataataa gactaatgaa atgtttgcaa
     4681 gtgttgcaaa ttttgatggg aaaatgaaaa tcaaagattc tgttaagttt gaaaatgtaa
     4741 aaaacttaag tgaattatat ataacattag gtaatgttgc ttcatctgtt ggaaattctt
     4801 ctgcagctat aaaaggtatt gtacttgata ttcctatatt gaaagataat aactatatta
     4861 cgcagatact ttatatgaaa gatgggaaaa ttaacaaagc ttttagtgat tatgataaaa
     4921 ctattactaa atcttattat atacctgtag atgatattga caaagataag attatagaaa
     4981 tacctatagt tgcaggcagt acaggtaata ataaaaatac ttatagttct aaatcatctg
     5041 caactataag ttggtataga tggaatggaa aagaaggtgc tgactcaagt ttaatattta
     5101 caagtcagat atattataat tataaatata attttaaatt atttattcca aataatttat
     5161 ttgataaaat acttgttgaa caggaatttg ttggagaaaa agcagtattt aaattttact
     5221 attttgacta ttcagaccgt aagaatttat ttaatatagt agtagagagt aaaaataaac
     5281 ttgaagatag aaaaaataca ggtactcaaa attctattgt acttcaagag agcgatgaat
     5341 atactttctt actagttgta aacaatgcaa atgaaatgga taagctagat atgactatag
     5401 acgcattaaa agaatatttc tcattaatat atggttaata ttatactcaa ttggaggttt
     5461 tattttatga aggaaaagat acttatacta gaagatgaaa ttggtattag aagttttgtt
     5521 agtataaact taaaaaggga aggctatgaa atagttgagg caggtactgg aagagaagct
     5581 atagaaaaaa tgacaacaga aaaggatatt acaatagctc ttttagatgt aatgcttcca
     5641 gatataagtg gtatagaagt atgtaaattt ataagggaga attttgacca agtaggtata
     5701 ataatgctta ctgctaaagc tcaagaagat gataaaatag aaggatttat ctcaggagca
     5761 gatgattaca tcataaaacc atttagtata aaagagcttt tagtcagagt ttctgctctt
     5821 cttagaagag tagcaaagga tgattcaagt gttaagtcga gtgagatagt ttctccacca
     5881 tttatacttg atatagacaa aagaaaatta tttaaaaatg gaaaggaaat tgagcttaca
     5941 ccaactgaat tttcaatagt gaaatatcta atatcaaatg ctaagcagtc gttaagtaga
     6001 gaccagatat tagacgaagt atggggaact aactatttat atgattttaa aatagtagat
     6061 gtaaatataa gaaggataag aaataaaata gaagatgacc catcaaaacc aaaatatata
     6121 caaactattt ggggttatgg atactgtttt agaaaggaag aataatagtg ttacattttg
     6181 aaggtctgag aaaaaaagta ataaaaaact attttataat aattattata atggttactc
     6241 tgtttgaagg attgttcatg ttttacatac aaaactacta ttatgattct gtcaaacagc
     6301 tacttgaatc tgaaataaaa tatgctgatg aatataatgc tattacaatg gaaactacaa
     6361 gttttgagaa aaaagtaaaa aatatatttg ataaacaacc cttaactaaa aattcagaat
     6421 ttggaatttc aataatagat aaagataaaa atattatatt agaccaatat ggttttaaaa
     6481 gtaaagaaaa agcaaactat gaagatgtta acaatgcatt aaaagatata aaaactaaaa
     6541 atttaacacc ttatacttat agaataccag atacagggga gcatgttatg agtatctcct
     6601 taccacttaa ggtaaataat ataatagaag gtgttgttag atatacagta tcattagatg
     6661 ctattgataa tgcaatatta aagcaagcta catggcttat attagcagga atatttatac
     6721 ttattatagc tatactaata agtcttaaat ttgctgagac tcttataaaa ccattacgag
     6781 agctaaagaa atttgcaaat gaattggctg taggaaatta taatataaaa ttagaaaaga
     6841 tgaagattgt agatgatgaa attggtgatt tagcacagac atttgaacac atggcacatg
     6901 agattgacaa aagtgaaaaa ttaaaagagg aatttatatc atctgtatct catgaactaa
     6961 ggactcctct tacatctata aaggggtgga gtgaaacctt aggttatgaa agtataacaa
     7021 gagaagaact agacttaggt ttaggcatta tacaagatga aacagaaaga cttataaaac
     7081 tagttgaaga attattagac ttttcaaggc tttcttcaga caggataaaa cttcatgttg
     7141 atatagttga tgttgaaggt ttaattgtag gtgttgtaaa tcaacttaaa gttaaagcag
     7201 ctgaaaaaga tatatcactt ttatttgaat ttgaaaatga atttatagag aacattcaag
     7261 gagataaaaa tagattaaga caggttctca taaatttaat acaaaattca tttaaattta
     7321 cgagtcaagg tggttacata aaagttgtag cttctcaaga tgaagagata acaaccatat
     7381 cagtagagga taatggttca ggtatagaaa agcaaaattt aaataaagtg ttagataaat
     7441 ttttccaaga agattataat aaggctggta gtggattagg actagcaatt agtaatgaaa
     7501 ttgtaaaact tcatggtgga agaatgaaaa tagaaagtga aaagaatgtt ggaacaaaaa
     7561 taacttttaa tataaaaaat aaatttgcta aacaagcata aattcttaga aagaggatta
     7621 agatgagaaa cataaaaatg atagtagcat acgatggttc aagatataaa ggatatcaaa
     7681 aattaggtga caataatatg actattcaag aaaaacttga aaatgtttta agtaaaatga
     7741 ctaatgaaac agtggagata ataggttctg gaagaactga tatgggagct catgcaagag
     7801 gacaagtggt aaattttaga actaattgta tggattcttt ggataaaatt caaaaatatt
     7861 tatatgaata tttaccagaa gatatagttg taaaaactgt tgaagaagtt gatgaaagat
     7921 ttcattcaag atacaatgta aaatcaaaaa cttatatgta taaaattgac aataataagt
     7981 atcatagtcc ttttataaga aaatatgcta ctcatgtaag taaaaaatta gatttagata
     8041 gaatgagaaa agcaagtgag tatttagtag gagaacatga ttttaccagt tttgcttctt
     8101 ctaaatctaa gaaaaaatca aatgtaagag aaatttattc tataaatata aaagaagatg
     8161 ataatgttat agaaatatat gtagaaggaa atggattttt gtataacatg gtaaggataa
     8221 tagtaggtgc tttaatagat gttggtctaa aaagaagagc tccacaagat attaagtata
     8281 tgcttgaatc aaaagataga tgtcaatcat cagatactgc accagctaaa ggactatgtc
     8341 tttggaaagt aacatattaa tcaattatat aaaactctct ttaaaataaa aataaaataa
     8401 attatatagt tgacataaat ggaaaaataa actattatat attttataag agagatataa
     8461 aactttatat ttaaatcctg tgagaaagag agtaacttct atgtgtttta tagagagctg
     8521 aggatggtgg aagcttggca aataagtatt agtgaaaaga actttggagg atactaattg
     8581 aatttagtat gttagtaggt aatctcgcct taagagaaag agtggataag tttatcttat
     8641 caattagagt ggtaacgcgg atatatttcg tctcttggtt tttccaagag gcgtttttta
     8701 atatatttaa ttattagtgt ttatctggta tgttgaacat ttactaattg attagctaag
     8761 tttatcaata agagtggtac cgcggatatt tcgcctctta gtattttaaa atactaaggg
     8821 gtttttttag tattaaaatg attttatgtg agtctaaatt ctcagagtca ttcaatatta
     8881 agaatttcta taaaataaaa attataattt aagattggga ggaattaaaa atgaaggaaa
     8941 aagtagtatt agcatattca ggaggacttg acacatcaat cataatacct tggttaaagg
     9001 aaaattatga agatatagat gttatagctg tttgtggaaa tgtaggtcag gaagataaaa
     9061 tggaagatgt atatgaaaaa gctttacaaa gtggagcatc aaaggcatat gtggatgata
     9121 taagtgaaga atttgtaact gaaactatat ttaaagccgt aaaagcagag gctaaatatg
     9181 aaggaaaata cttattagga acttctttag caagacctat aatagctaaa aaattagttg
     9241 aagttgctca taaagaaggt gcaaaatata tatgccatgg atgtacagga aaaggtaatg
     9301 accaagttag atttgaagct acaattgcag ctcttgaccc gactataaaa gttattgcac
     9361 cttggagaat ttgggatata aaatcaagag aagatgctat agactatgct gaaaaacata
     9421 atataaaggt aacagcaact aaagctaaaa tatattcagt agatgctaac ctttggcatg
     9481 tttcaacaga aggtggagat atagagcacc tagaaaatga acataaaaaa gatgtttata
     9541 aacaatgtgt agacccagaa gatgcatgtg atgtagctga atatgtagaa gtttactttg
     9601 aaaaaggtgt acctaaaaag gtaaatggtg aagaattatc tccagttgca ctgattcata
     9661 agttaaatga gttgggttgt aaacatggaa ttggtgttat agatatagtt gagaatagac
     9721 ttgttggtat gaaatcaaga ggtatatatg agactccagg aggaacaatc ctttatgaag
     9781 cacataatat tttagaaagt gctactttag acaaagatac attacatttc aaacaaatgg
     9841 tatcttataa atacggagaa cttatttaca atggactatg gtattgtaaa ttaagagaat
     9901 ctatagatgc atttatggaa caaactcaag ataatgttac aggaacagta aaagttaaac
     9961 tttacaaagg aaatataaaa cctgctggaa tatttactga aaatgctctt tatgatgaag
    10021 gaatatcttc ttttggaaat agtgaacttt atgaccacaa agatgcagaa ggatttatta
    10081 atttattcac tttaccatta aaaatcagag ctatgaaagc tggaaaataa taaaataaat
    10141 tatttaagag tgtataaaaa ctatttttag gtttttatat gctctttttt tatttgaatt
    10201 tattaactta tacagtaaat agtataaaaa aatatattat agttccaaat ggcttaataa
    10261 tgtgaaaata taagacctaa aatataaatt ttataaatac aatagttatt gactctctca
    10321 ttgtaagagg gtttatactg aatctaatga aaatttatag tgaggagata gattatatga
    10381 gaaaaataat tgaaattaaa catcattcac atgattatga gtgtatgtgg aatggtatag
    10441 aagatttata tataaataaa accggtgaat gccttccaaa cgattttttc tttttactgt
    10501 caggttttgg ttcattttgt tatatgaaaa caaataaagc tgatttgaaa agaatggtag
    10561 ctttgggaga tggaaggaca aaacagatgt ataaattttt agcaccaatt gttggatttg
    10621 aatacaaaca tcatgagttt aaaaagtatg agcaagcatt aaagaaagca aaatctgaaa
    10681 ttgatgatgg atatccagtt attttaggag ctttagatat gtattatcta ccatattata
    10741 agaagctata tcataaagaa catattcctt ttcattatat attaatggta ggatatgatg
    10801 atgaaggaat atatctgtat gattgtggta gaacagaatt attacatctt tcttatgata
    10861 aattacgtga aagcatgaat tgcaattccc ctggattaag caaagaaaat actatttgca
    10921 caataagaat gaatacttca aagaataaat ataacattgc tagagaagct attgctataa
    10981 agcgtgatat gtttttaaat ccacctaaag gatttttggg atataaaggt tttgaaaagt
    11041 ttattaaaga gttgccacag tggaacactg aattggataa agatgattat gataagattt
    11101 tgaccaatat ggttatgttt tttggaacag taccaacaat tccaaatgca atcaaaggaa
    11161 ttaaagaacc agatactgtt caatttaaag gtggatttga taaaatgagt agaatgctca
    11221 acagtattgg ggaggaatac aaaaacgact cttttataca agtagctgat atttttgata
    11281 aaactgctat tataattgag aaaatatcca atattataat agattactta actcaaacat
    11341 gtgatgatac aggacaactt ccgttattgt tctctgaggt cttagagcat atgaaaacag
    11401 gatatttaat cttggatgta taaaaaatat attgttgacc ctaacattac gtaatagtgt
    11461 aatatgaaaa tatagcttga atatattagt aggtattgat ggctgtaaaa ttaaaatttg
    11521 aggtgtggat atgagaacag tgaaacaagt ttctgattta actggaataa gtgtgagggc
    11581 attacattat tatgatgaaa taggtctttt aaaaccaaat aaaattacag atgcaggata
    11641 tagactttat gatgatgaat ctattaaaac tcttcaacaa attttatttt ttaaggagat
    11701 tgatattcct ttaagagagg ttaaggaaat aatgtcaagc caatattttg ataaggttga
    11761 agctctaaaa aatcaaaaaa aattacttat tttaaaacga aagagactgg atgaattgat
    11821 agagcttata aatcaaactc taagaggaga aggaaatatt gattttaaag agtttgatat
    11881 gagcgagtat tttgatgttt tagaagaatt taaaagagag cataggaata aggtaattaa
    11941 aatttatggt agtgtagaaa aatacaatga atatatagaa agagttaagt ctaatgaaga
    12001 gaaaattgct aaaatggcta taaaacaata tggaactatt gagaagtttg ctaaagctat
    12061 aaaaacaaat tttagcagtg atatattgaa tttaggagaa aaatttgata gatataaaaa
    12121 tgattgttta aaagaaaaac atccaaaatt aaaagaatta tatagaaagc ttgtagaaga
    12181 tttaaatagg aatccttctt caacagacct tcaagaaata gcaaaagaga taacagatat
    12241 atctaaaaaa gattatgaaa ttttctctat ggatactggt gatgacaact ggtattatat
    12301 ggttcaaaat tatctagtaa atcctatgtg gatagaagaa gtagataaaa agtatggaag
    12361 tggagcaggt aagtttattg gacaagtact aaagacttat ctaagagata gaaaaccaaa
    12421 gataaataca ttgtatgaaa aacttgtaga agatttgagt agagattgtt cttcaaggga
    12481 agttcaaagc attgttgaag aaatagacaa tgaaatgaaa agaagtaatg aattttataa
    12541 aatagacaat ggtgaaagat actttgatta tatgtcagag ctttatttac aagattctaa
    12601 ctatataaaa gtaactgata agaattatgg tgatggtgca tctaagttta ttggagaagc
    12661 ttttaagatt tattttgata ataataattg ttgaaaattt ggaagtaaaa aatatgcaag
    12721 ttttataaaa gcacaaaaat aaggatgtag attagctcca tctatatcct tatttaaagt
    12781 tattaaaata tatagtttac atcaaactta acactttcaa taatatttcc caaatatgtt
    12841 tttatacttt caacttcttc taaagatttt aatttatttt cagtataaat tgctttgtcc
    12901 agaatattaa accttaatcc tatttcatca aagtactcat gatttaaaac tttgtaccaa
    12961 tggttttttt ctttattcca actcgtagat attgaatcag cttctttttg agctttttcc
    13021 caatcttcat tttctataga tttttctaaa acagatatat tgtctttata tccttctgta
    13081 aaatcatata gtttattgtt aacataaaat ccaaataata caaataagat agtccatagt
    13141 ataacaaatg ttgtagattt caatattaat cacccttttt attttgaata aatattttgt
    13201 cattttcatc taaaacacaa ataagaacat ctttaaaagt ttctatgtga ttagatttta
    13261 aaatacccat taaccagtta gtatcttttt gagctatgtc tagattttta tttattattc
    13321 taccatccag tataagtggg ataggtaaat gattaaaagc tcttggagga gtactattgt
    13381 agcttgatgc tggtacaaca cttagattac catcagtctc caaaatagca tattgtacat
    13441 cttttaggtt aaagtatcct tgaatcctaa gttgttctaa taattcatct atactaattc
    13501 tttctttttt aagctcttta tatacaattt ttcctttatc tattaataca gaaggtttac
    13561 ctgataaaaa tgaacttaat tttctacttt ttaatgataa gaatgaaatt agagtctgca
    13621 tgattacaag tccagatatt gctgcaacac catttatgat tggtatatta ttattttcca
    13681 ttgggacaga tgcaacttct gcaattaata gtgttataac taaatcaaaa cagttcattt
    13741 cagctatttc accttttccc atgactctaa gtgcaattag cactgttatg tataatataa
    13801 tacttcttat taatactact gtcatatcta tcaattactc ctttaaaaaa tatttacact
    13861 tagttagtat atcctatata aaaaatttaa ttcaaagtat aaaaaacctt aaataattgg
    13921 attcaactat tt
//
LOCUS       DAYAPA010000117        13891 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.119, whole genome shotgun sequence.
ACCESSION   DAYAPA010000117 DAYAPA010000000
VERSION     DAYAPA010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13891
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.119"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(<1..917)
                     /locus_tag="ACGI59_003049"
     CDS             complement(<1..917)
                     /locus_tag="ACGI59_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HFG9740827.1"
                     /translation="MKYKLFIDFEFNILDDNKNKPKEYNGAELISIGGVLVDNEFNTI
                     DNYYSLVKPKYNKILSNKCKNLTKLNQLDINNAPNLLYVMDDFYKWFCKFDDVTIYNW
                     GDFDITGLLTSFRVYKYTGKCLELFNMMIDIQPFISQHITYNNRILSKQISLLNMKKI
                     FSVEGDIKHNSLSDAVDLMNVCKCFFFNSPKNIDILEELYNKLPALKTPLYYIPYFED
                     ENFELKFDRTPEDIIIYLKQILTILNISKKEIYFKKRSVLVNNSKIINFKNLSSSFKL
                     IKLNEFEEYPDFILKLGDKKSFVESVIKI"
     gene            complement(957..1304)
                     /locus_tag="ACGI59_003050"
     CDS             complement(957..1304)
                     /locus_tag="ACGI59_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9740828.1"
                     /translation="MLNLKEIREEIIKKYIGDSLISELTEELKNILITDLQSNPFKSN
                     IELSIIPANIEIFKKIENILIDSKVNLKMGWERILINYILKDLFELNYEINKSCNEPL
                     MFRVIISIDSYDA"
     gene            complement(1522..2478)
                     /locus_tag="ACGI59_003051"
     CDS             complement(1522..2478)
                     /locus_tag="ACGI59_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFG9740829.1"
                     /translation="MKKILITGALGQIGSELTIKLRNEYGEQNVIASSRRVKEGNPVC
                     ESGIFEILDVTDKNRFFEIAKKYDVDTIIHLASLLSAVAESKPLEAWNLNMNGLINGL
                     EIAKELDCKFFTPSSIAAFGENSPKNMTPQDTLQRPNTMYGVTKVSGELLCDYYHSKF
                     GVDTRGVRFPGLISYVTPPGGGTTDYAVDIYYEALKNKRYKSYIAEGTKMDMMYMPDA
                     LQSIVDLIEAPADKLIHRNAFNITAMSFSPEEIADSIKKYIPDFVIEYDVDPVRQSIA
                     DSWPNSLDSSSAVKEWNFKFSYDLDKMTKDMLEKLSEKGIGK"
     gene            2923..3291
                     /locus_tag="ACGI59_003052"
     CDS             2923..3291
                     /locus_tag="ACGI59_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family (seleno)protein"
                     /protein_id="HFG9740830.1"
                     /translation="MTRPSIYHSQGYTCAEALIKSYNEEHNTDIPISIGSGMGVGMNV
                     GSVCGAVNAAVVIIGYLNGRNSNLDENIARKYSRELLNRVRNKYSSEICVSLKKNKVT
                     CEEIINFTYDALNDMLKNQK"
     gene            3716..3961
                     /locus_tag="ACGI59_003053"
     CDS             3716..3961
                     /locus_tag="ACGI59_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9740831.1"
                     /translation="MFIDEELEGYILTCKISEDFKNIPEYSDEEFYVTVYKDESSDSG
                     YYALLENKEERVVWDGEVVANNIFNNLWIVVNKVKTG"
     gene            4060..4323
                     /locus_tag="ACGI59_003054"
     CDS             4060..4323
                     /locus_tag="ACGI59_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1883 domain-containing protein"
                     /protein_id="HFG9740832.1"
                     /translation="MEYSYSKMNLKKGDIVEVNLEKQANVILLDHINYVKFKNQRNYD
                     YYGGFAKKNPCRMRVPNTGTWYLVVNQDGNSGIVNFSINTIQN"
     gene            complement(4608..5555)
                     /locus_tag="ACGI59_003055"
     CDS             complement(4608..5555)
                     /locus_tag="ACGI59_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFG9740833.1"
                     /translation="MSNVTAEQKKEVKGRGFLPSRDGEHFAARIITVNGVINASQAKK
                     IAEAAEKFGNGQLAFTTRLTVELPGVKFEDIEALSEFIASENLITGGTGSRVRPVVAC
                     KGTVCVHGLANTQALAAEIHEEFYKNWYDVKLPHKFKIGVGGCPNNCIKPDLNDLGIV
                     GQRVPDYDSELCVGCKKCAVVEVCPVKAAKLTDKGKLEIDSNLCNNCGKCIESCNFDS
                     IEEKESGYKVYIGGKWGKSVRPGTQIDRLFSKEELMTLIEKAILLFREQGNTGERFGI
                     TIDRIGADKFIEMLLSDEVLERKEEILEALLHLTGGAVC"
     gene            complement(5558..7189)
                     /locus_tag="ACGI59_003056"
     CDS             complement(5558..7189)
                     /locus_tag="ACGI59_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFG9740834.1"
                     /translation="MKVLIIGGVAAGTKTAAKIKREMGDNCSVTILNKGDDISYAGCG
                     LPYYVGKIIEDKSSLIVNTPESFSKLTGAEVRCGVEVTSIDRNAKQVTLKTLSTGEKE
                     NLSYDKLVIATGADPIKPPIPGIDLPGVFFMRTPNDAIKLRDSIENGIKRAVVIGGGF
                     IGLEIAENLSAMGIRVSVVDMAEHVMPGFDTDFAEYVENHMADKGIMIFTGDQVVGIE
                     GENKVEKLRTKNRAIKTDLVVMSVGIRPNTGFLKDTGLEFAPNNTIIADEYMLTNDKD
                     IYVVGDCAFVKNSLTNKPTWSPMGSSANHEGRICAQNISGKSKTYNGVLGTTVVKLPE
                     LNAGKTGLSKEIAEKEGYNVCSVTIATDDKAHYYPGASNFIIRMVAEKNTRKLLGVQV
                     MGPGAVDKIVDIAATAITLGADLYSLESMDLAYAPPFSTAIHPFVVAVNVLQNKLNGE
                     LDSVLLDEIEDFDSWTKLDVSKAPSIPSLRYIPVGEINGEIEGLAKDEKILLICAKGK
                     NSYMAQNRLRRFGYTNTKVLEGGILFYPNLKVMEE"
     gene            7319..8236
                     /locus_tag="ACGI59_003057"
     CDS             7319..8236
                     /locus_tag="ACGI59_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFG9740835.1"
                     /translation="MTIRSLEIFVKVAECGKMSEVARNMYITQSSVSQAISEIEKEYG
                     VKLFDRISKKLYLTEAGKKLLGYGRHLLAVNEEMNDCMKHCAKNIRIRVGATVTVGTC
                     VISPIMLELYKVNPLIEPEVFVEDTRLIAKKLLNSELDIAIVEGKIKHPDIVTKSIIN
                     DNLVLICSHKHEFYKRDSIKVSELSNQPLIMRELGSGTRAQLEKQLKELKIPMNIRWS
                     CYNSEAILRAVVDNFGIAVISELLIEDYLKKHLLWACDIEGINLHRTFDIAYHRSKFF
                     TENISAFFDISVEYGKKQARKKSRSVNSL"
     gene            complement(8293..8874)
                     /locus_tag="ACGI59_003058"
     CDS             complement(8293..8874)
                     /locus_tag="ACGI59_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFG9740836.1"
                     /translation="MGITLAKGQKVSLTKSNPGLKKVIVGLGWDINKYDGGFDFDLDA
                     SAFLTGTDGKVTNDGDFIFYNNLKHASGAVEYMGDNRTGVGDGDDEQINVDLSKIPQN
                     IAKISFSVTINEAITRRQNFGQVENSYIRIYNEETNEELIKYELGEDFSIETAIVVAE
                     LYRHNGEWKFNALGSGFEDGLAGLCKNFGVNIG"
     gene            complement(8914..9480)
                     /locus_tag="ACGI59_003059"
     CDS             complement(8914..9480)
                     /locus_tag="ACGI59_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFG9740837.1"
                     /translation="MSVSLQKGQRVSLVKDNNPVKNLVVGLGWDMNKLGKKNYDLDAF
                     AIALTNQDKMNVRGDLVYFGNLKHPSKAIIHTGDNLTGKGEGDDEQIIVNLEDIPEYV
                     HKIVFGVSIYKAKKRDQDFGQINNSFIRLIDSNSKQELFKYNLQEDFSGKVTVLAGEI
                     YRRNEEWKFNALGIGQNEELRELINTYK"
     gene            complement(9936..10796)
                     /locus_tag="ACGI59_003060"
     CDS             complement(9936..10796)
                     /locus_tag="ACGI59_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFG9740838.1"
                     /translation="MLLKKDRITFSEILILIVGCILMAISLNLFFNPHAIAAGGITGL
                     GVVLNSLFGVELWIVNLLLNVPLFIFAYKILSKKDCFKTVLGIIFLTIALKLTANMAT
                     LDITNDMYLAIISGSILMGVGQGLIFRINGSTGGTDLMALLLNKYFPTFSIPVLMGIV
                     DCVVVVLSGIVNRQVEIALYSTVALYILVKVSDLLIEGFNYSKSFTIISDLSKDISKK
                     IMEDLDRGATILKGEGAYTGENKNVLLVVVEKKEVVELKKLVKNVDPNAFIIITDIHE
                     ALGNGFKKIE"
     gene            10945..11835
                     /locus_tag="ACGI59_003061"
     CDS             10945..11835
                     /locus_tag="ACGI59_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFG9740839.1"
                     /translation="MAKLTLEHIKGNTYYIPLPTIVGVYVDGKDAILIDSGNNKDTAR
                     QVLRLLEEHNLNPKLIINTHSNADHIGGNAYLKNQTKCKIATTKIEGYFTENPILESA
                     FLYGGYPSKALKNKFLLAKESEVDYIIPSNGKIVDTELEAISLPGHYFEMIGVRTPDN
                     VLFVADSLTPENIITKYHFFFLLDIDSQFKTLEKLRMLEADFFVPSHSVKTTDIKSLI
                     NINKKKMEEIIDNIKKVCYEPVMIDEVIEKMCDLYNVKLDANQYVLVGSTIRSYITYL
                     YENNMVEYIFDGGKMMIKAV"
     gene            12053..13807
                     /locus_tag="ACGI59_003062"
     CDS             12053..13807
                     /locus_tag="ACGI59_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp23"
                     /protein_id="HFG9740840.1"
                     /translation="MYIMNKKFKKFSKKLSILILVLIMFLFNNLTANAQIKEDSIIGI
                     DRYETAGLIADRQTYDTVILVNGDKNLSDGLSSSGLAGAINAPILLTKKSEIPKATLN
                     RLDNKSLNVVKKVYIIGGYNTIENSVEKDIKRKGIQVERINGNNRIETSYNVAKKINE
                     VSSVKEVMLTNGFVGEADAMSIAPVVAKNKGAIILTDGKSVPFKTESLNVYAIGGKSA
                     INEDLVNETKATRIGGNDRFETNKKVMEKFYNGATDFYITNGYQLVDALTLSPLAKEK
                     PIVLVADGSNKGILKGAKSITKVGGIDANIYKQCLDMIKYNNMDMTPNILKHLTSVEG
                     NEVSEILIESDNLGVVVEKTNTNKFEFDYVSVTNEKNCTFSVYKENSSNNIKNGKLVV
                     SAKKKIDKKDSDLQDMNGDNMINANKDRMVNVIKIGVPDKMYSNFKVDVESGSVELYN
                     IKGGATVDVNDGIANIVDNNVTNPFNINTNSGISGVTAETISSEIKFRANNGIVNVTA
                     TNINGNISLFGSDNKGTFDGIFKLNLKKEPSNLHLKLIGNGINKLPEGWSKDYILGNG
                     QPIIEVKNNGINNISIGE"
BASE COUNT         5133 a         1873 c         1912 g         4973 t
ORIGIN      
        1 tttattttaa taacagattc aacaaaactc tttttatctc ctaattttaa gataaaatca
       61 ggatattctt caaattcatt gagttttatt aatttgaaag aagaagatag atttttaaag
      121 ttaataattt tagagttatt cactaataca cttctttttt taaaataaat ttctttttta
      181 cttatattta gtatagttaa tatttgttta agatatatta ttatatcttc tggagttcta
      241 tcaaatttga gttcaaaatt ttcatcttca aagtagggaa tataatataa aggtgttttg
      301 agagcaggta atttattata taattcttca agaatatcga tatttttagg ggagttaaag
      361 aaaaagcatt tacatacatt cattaaatcc acagcatctg aaagtgagtt atgtttaata
      421 tctccttcta cagaaaaaat ttttttcata ttcagtaaag atatttgctt agataagatt
      481 ctattattgt atgtaatatg ttgagatata aaaggttgaa tgtcaatcat catattaaat
      541 aactctaagc atttacctgt atatttatat actctaaatg aagttagaag gccagttata
      601 tcaaaatcac cccaattata tattgtaaca tcatcaaatt tacaaaacca tttataaaaa
      661 tcatccataa catataaaag attaggagca ttattgatat ctagttggtt caatttggtt
      721 aaatttttac atttatttga aagtatttta ttgtattttg gcttaactaa agagtaataa
      781 ttatctattg tattaaattc gttatcaact aaaactccac caatagaaat taattccgca
      841 ccattatatt cttttggttt atttttatta tcatctagta tattaaattc aaagtctata
      901 aataacttgt atttcataaa tctaaagcac atcctcaggt aaattattta ttgagcctaa
      961 gcatcataag aatcaataga tataatgact ctaaacatca aaggttcatt acaactttta
     1021 tttatttcat aatttaattc aaataaatct tttaagatat aatttataag tattctttcc
     1081 caacccattt ttaaattaac tttagagtct attaatatat tttctatctt tttaaatatt
     1141 tctatatttg ctggtattat actgagctca atattacttt taaacgggtt agactgtaaa
     1201 tctgttataa gtatattttt taattcctct gtaagttcag aaattaaact atcacctatg
     1261 tattttttta tgatttcctc tctaatttct tttaaattta gcaatattag tacctgctac
     1321 ttacatagta gcttttcctt tccttttaat actattattc atttttgaat cttatttaga
     1381 tatattttaa gattaaaagt ctttagctat ataatttttt acccattttt aacttatatt
     1441 aacatattac aggcttatat ctccaccctg tttacattga ggatggagta tattgcctat
     1501 tttgataaaa atgataggtt gctatttgcc tattcctttt tctgataatt tttctaacat
     1561 atctttggtc attttatcta aatcataact aaacttaaag ttccattctt ttactgcact
     1621 actactgtct agagagttag gccaagaatc agctatactt tgtcttacag ggtctacatc
     1681 atattctatc acaaaatcag gaatatattt ttttatagaa tcagctattt cctcaggaga
     1741 aaagctcata gcagtaatat taaaagcatt tctatggatg agtttatcag ctggagcttc
     1801 gattaaatcc actattgatt gaagtgcatc aggcatatac atcatatcca tttttgtacc
     1861 ttctgctatg taagatttat atctcttatt ttttaatgcc tcataatata tatcaactgc
     1921 ataatctgta gttcctccac caggaggcgt aacataagaa ataagacctg gaaatctaac
     1981 acctctagtg tctactccaa attttgagtg ataataatca catagtaatt ctccagaaac
     2041 tttagtcaca ccatacatag tattaggtct ttgtaatgta tcttgaggag tcatattttt
     2101 tggagaattt tcaccaaaag cagcaattga actaggtgta aaaaatttac aatccaattc
     2161 tttggctatt tcaagaccat ttattaatcc attcatattt aagttccaag cttctagagg
     2221 tttactttca gcaactgcag aaagtaaaga tgctagatga attatagtat ctacatcata
     2281 tttttttgca atttcaaaaa aacgattctt atctgtaaca tctaatatct caaatatacc
     2341 agattcacat actggattac cttcttttac tctccttgaa ctagcgatta cattttgttc
     2401 tccatactca tttctaagct ttattgttaa ttctgaacct atttgtccta atgcacctgt
     2461 tataagtatt tttttcaatt ttacaacctc ctaagggttt aatttatgta gtactactta
     2521 tttttatttt agcaaatctt ttgtttaaat acaaagaatt aaagataata atatacgact
     2581 taattgaatg aagtgcagta agtgcctaaa aatttaaagc taatatataa tggtatattg
     2641 tataacaata aaagagtaga ttaaattatt atatatagat tgtgtgacta agtcacagaa
     2701 aatagagtta atttaatata taataaaagc atatcaagta aagctttaaa ttaaagtaac
     2761 tttataaata aaaagtaata taaattgata aaattataaa tttatttata gtgaatatgg
     2821 gttggatttt atagatacaa aaatagtata tatgatatgt agaccattag caggtttaaa
     2881 gtagcaaaac atataaaata aaatttgaaa ggaagtataa aaatgacaag accatcaata
     2941 tatcatagcc aaggatacac ttgtgcagag gcattaataa agtcttataa tgaggaacat
     3001 aacacagata taccaatctc aataggaagt ggtatggggg ttggaatgaa tgttggaagt
     3061 gtttgtggag ctgtaaatgc tgctgttgtt ataatagggt atttaaatgg gcgaaatagt
     3121 aatttagatg aaaatatagc aaggaagtat tcaagagagc tattgaatag agtaagaaat
     3181 aaatacagct cagaaatatg tgtatctctt aaaaaaaata aagtaacttg tgaagaaata
     3241 ataaatttca catatgatgc tctaaatgat atgctaaaaa atcaaaaata attctaagaa
     3301 acaagtattt tacattcgat agaatacttg tttttttatg atatttttaa taagtattag
     3361 tttattataa tttttaaatc aattttatta tattttaatt gaaacaatct actttgaaaa
     3421 ttactaactg acaaaagaat atgattatag tatagaaaaa atgtaaatta tttaatatta
     3481 agtgagagta tatgtttacc tatataaaat cgtataaatt aatggctaaa tttataaaat
     3541 ataaagaaat atataggaaa acaaaagaat taattagtat aatataaagt ttaaaagtta
     3601 aaatttatat aatttattta taaagtaaag aaaaaataga aatttgtaat aaaataaaaa
     3661 gtttatgaca taatatggta taattagtaa aaatatagag attggaggaa ttgctttgtt
     3721 tatagatgaa gaattagaag gttatatatt aacatgtaaa atatctgaag actttaaaaa
     3781 tatacctgaa tatagtgatg aagagtttta tgttacagtc tataaagatg aaagttctga
     3841 ctctgggtac tatgctttat tagaaaataa agaagaaaga gttgtatggg atggagaagt
     3901 tgttgccaat aatattttta ataacctttg gattgtagta aataaggtta aaactggata
     3961 atagtctaat tatatagtat ttgtaatatt ttaaatgaat ttttacatat ttgagatgag
     4021 taaataacaa agattattcg atactggagg agtaattcaa tggaatatag ttatagtaag
     4081 atgaacttaa aaaaagggga tattgtagag gtaaatttag agaaacaagc caatgtaata
     4141 ttgttagacc atataaatta tgtgaaattt aaaaatcaaa gaaattatga ttactatggt
     4201 ggatttgcaa agaaaaatcc atgtagaatg agagttccta atactggaac atggtatcta
     4261 gtagtaaatc aagatggaaa tagtggaata gtaaactttt caatcaatac aattcaaaat
     4321 taattttaga atattggata ttcttaattt ttgtacattt tatatagtta aaataaaatt
     4381 aataagcaat tattataaaa taatgtgggt gcctcaaaat gaattttata attcatgaga
     4441 cactcttttt tatatgaact caaatatact ttatcatttc aacaatgaaa aagaggcgta
     4501 tctgtgtttt gagatagcct catttatcat atatcatata tagaatttta attattctta
     4561 actaaaattt tttccatagg ttatctgtac aattaaaata tttattgtta gcatacagct
     4621 ccacctgtaa ggtgtaaaag agcctctagt atctcttctt ttctttccaa aacttcatct
     4681 gataaaagca tttctataaa tttatcagca ccgattctat cgatagttat tccaaaacgt
     4741 tcacctgtgt taccctgttc tctaaataaa agaattgctt tttcaattaa agtcataagt
     4801 tcctcttttg aaaataatct atcaatctgt gttccaggac gaactgattt tccccatttc
     4861 cctcctatat agactttata accagattct ttttcttcaa tactatcaaa attacaactt
     4921 tctatacact taccacagtt attacataga ttactatcta tttcaagttt acctttatct
     4981 gttaatttag ctgctttaac aggacagacc tctacaacag cacatttttt acaaccaaca
     5041 caaagctctg aatcatagtc aggtacacgt tgaccaacaa ttccaagatc gtttaggtca
     5101 ggtttaatac agttattagg gcatccacca acaccaattt tgaacttatg aggtagtttt
     5161 acatcatacc aatttttgta aaattcttca tgtatttcag cagcaagtgc ctgtgtatta
     5221 gctagaccat gaacacaaac agtaccttta caagccacaa caggacgaac ccttgatcca
     5281 gttccacctg ttattagatt ttcgctagca ataaattcac ttagagcttc aatatcttca
     5341 aattttacac caggtaattc tactgttagt ctagtagtaa atgctaactg accgtttccg
     5401 aatttttcag cagcttctgc aatttttttt gcttgtgatg catttattac accatttaca
     5461 gttattatac gtgctgcaaa atgttctcca tccctgcttg gtagaaaacc tctacctttt
     5521 acttcctttt tttgttctgc tgtaacatta ctcatattta ctcctccatt accttaagat
     5581 ttggataaaa tagtattcca ccttcaagaa ctttcgtatt tgtatagcca aagcgtctta
     5641 agcgattttg cgccatatag ctatttttac ctttagcaca tataagaaga attttttcat
     5701 ccttagcaag accttcaatt tctccattaa tttcaccaac aggaatataa cgtaaactag
     5761 gaatagaagg agcttttgaa acatcaagtt tagtccaact gtcaaaatcc tcaatttcgt
     5821 caagtaaaac actatcaagt tctccattaa gtttattttg taaaacattt actgccacta
     5881 caaaagggtg aatagctgtt gaaaaaggtg gtgcatatgc aaggtccata ctttctaatg
     5941 aataaaggtc agcgcctaaa gttattgcag tagcagcgat atctactatt ttatcaaccg
     6001 ctccaggtcc cataacttga acacctaata attttctagt atttttttca gcaaccattc
     6061 ttataataaa attagatgca cctggataat aatgagcttt atcatcagta gctatggtta
     6121 cagagcaaac attataacct tctttttcag ctatttcttt acttagacct gtttttcctg
     6181 cattgagctc aggtaatttt actactgttg ttccaagaac accattgtaa gttttagatt
     6241 ttccagatat attttgtgcg caaattctac cctcatgatt tgcagatgac cccataggtg
     6301 accatgtagg tttatttgtt aaagaatttt ttacaaaagc acagtctcca actacatata
     6361 tatctttgtc atttgtaagc atatattcat ctgcaataat tgtattatta ggggcaaatt
     6421 ctaaacctgt gtcttttaga aaacctgtgt taggacggat tccaactgac ataaccacta
     6481 aatctgtttt aatagctcta tttttagtac gaagtttttc aactttattt tcaccttcaa
     6541 tacctacaac ctggtcacct gtaaaaatca ttatgccttt atcagccata tgattttcaa
     6601 cgtattcagc aaaatcagta tcaaatccag gcataacatg ttcagccata tcaacaacac
     6661 ttacacgtat acccattgca gaaagatttt cagcaatttc aagaccaatg aaacctccac
     6721 caataacaac agctctctta ataccgtttt caatagaatc tcttagtttt atagcatcat
     6781 ttggagtacg cataaagaat actccaggta aatctattcc aggtattggt ggttttattg
     6841 ggtctgcccc tgtagcaatt acaagtttgt catagcttag attttctttt tctcctgttg
     6901 aaagcgtttt caaagttact tgttttgcat ttctatcaat agatgtaact tcaactccac
     6961 aacgaacttc tgctcctgtt agtttagaaa aactttcagg tgtatttacg attaaagatg
     7021 atttatcttc aattatctta ccaacgtaat atggaagacc acaacctgca taagaaatat
     7081 catcaccttt atttagtatt gtaacagagc aattatcacc catttcacgt tttatttttg
     7141 cagcagtttt tgttccagca gcaactccac ctataattaa tactttcaca atatatcaca
     7201 ctccaatcta ctttataact ctataataaa attatacttc ttgacaatat atttgtaaaa
     7261 tactaaaata ttaataaaat cataagcatt tactaatgtt tgctaaggtt gtgataatat
     7321 gacaattcgt agtcttgaaa tctttgttaa ggttgctgaa tgtggtaaaa tgagtgaagt
     7381 agcaagaaat atgtatatta cccagtcatc agttagccaa gctatttcag aaatagaaaa
     7441 agagtatggt gttaagctat ttgatagaat ttcaaaaaaa ttatatttaa ctgaggcagg
     7501 gaaaaaactt cttggatatg ggaggcatct tttagctgta aatgaagaaa tgaatgattg
     7561 tatgaagcat tgtgcaaaaa atatccgtat aagagttggg gcaacagtaa ctgttggtac
     7621 atgtgtaata agtccaataa tgttagaatt atacaaggta aatcctctta tagaacctga
     7681 agtatttgta gaagatacaa gactgattgc aaaaaaatta cttaatagtg aattagatat
     7741 tgcgattgta gaaggaaaaa ttaagcaccc agatattgtt acaaaaagca ttattaatga
     7801 taatcttgta cttatatgtt cacacaagca tgagttttat aaaagagact ctataaaagt
     7861 ttcagaactt tcaaatcaac cattaattat gcgtgaattg ggaagtggta caagagcaca
     7921 attagaaaag caattaaaag aattaaaaat cccaatgaat attagatggt catgttataa
     7981 ctctgaagct atattaagag cagttgtaga taattttggc attgcagtta tatccgaatt
     8041 acttattgag gattatttaa aaaagcatct tttgtgggct tgtgatattg aaggtattaa
     8101 tttacatagg acatttgata ttgcatatca tagaagcaag ttttttacag aaaatatttc
     8161 agcatttttt gatatttcag tagaatatgg gaaaaaacaa gcgagaaaga agagtagaag
     8221 tgtaaacagt ttataatata tctaaataaa aataagagta tatcaatata ctcttatttt
     8281 tatttagata tattagccta tattaacgcc aaaattttta cacaatcctg caagtccatc
     8341 ctcaaatcca gaacctaaag cattaaattt ccactctcca ttatgtctat atagttctgc
     8401 cacaactatt gctgtttcta tgctaaaatc ttcacccaat tcatatttaa taagttcctc
     8461 attagtttct tcattatata ttcttatata tgaattttca acttgtccga aattttgtct
     8521 tcttgtaatt gcttcattta tagtaacact aaatgatatt ttagcaatat tttgaggtat
     8581 tttagataag tctacattta tttgttcatc atcaccatca ccaacacctg ttctattatc
     8641 acccatatat tcaacagctc cagaagcgtg ttttaaatta ttatagaata taaagtctcc
     8701 atcattcgtt acttttccat cagttccagt taaaaatgca gaggcatcta aatcgaaatc
     8761 aaagccacca tcatatttat taatatccca tcctagtcct actattactt tttttagacc
     8821 agggttactt tttgttaaac ttactttttg tccttttgct aaagttatac ccatttaaac
     8881 caactcctca ttataaattt tttattgtaa ttattactta tatgtattaa ttaattcacg
     8941 aagctcttca ttttgaccaa ttcctaaagc attaaatttc cattcttcat ttctacgata
     9001 aatttctcct gccaaaactg ttactttacc agaaaagtct tcttgaagat tatatttaaa
     9061 taattcttgc ttggaatttg aatcaattaa tcttataaaa gagttgttta tttgaccaaa
     9121 atcctggtct ctttttttag ctttgtatat agaaactcca aaaactattt tatgtacata
     9181 ttcaggtatg tcttctaaat taacaattat ttgttcatca tcaccttcac cttttccagt
     9241 taagttatct ccagtgtgta taatagcttt agaagggtgt ttaagatttc caaaataaac
     9301 caagtcacct ctaacattca ttttatcttg gttagtcaat gctattgcaa atgcatctag
     9361 gtcataattt tttttaccca atttattcat atcccatcct aagcctacta ctagattttt
     9421 tacaggatta ttatctttta ctaagctaac tctttgtcct ttttgtaaac taacactcat
     9481 tttaagttct cctttttata tttttagttt tattgatggt atcaagtata agaaccatat
     9541 tagctatatt atatacgtat ttaggctaag ttggctatat ttataaatta tttattatat
     9601 aactacatga atattttata atttaaacat attgacatta tatgctttaa attattttgt
     9661 agttttataa tatttaaatt tgtatttgat tgttataaag taaagtaagt aataaagtta
     9721 gactcaataa ttctaaaatt tctaagcata ctatctaaat gagaacggat ttggaaatac
     9781 ttgtataaaa caataattga tacatttaat ttttatctta aaaacaacaa ttataatata
     9841 taaatagcaa gtataatata taaatagcaa gtataatata acatataaaa agttgaattt
     9901 gtatatgtta attatacttg ctatttattt gttagttatt ctattttttt aaatccatta
     9961 cctaaagctt catgtatatc tgtaattata ataaaagcat tagggtcgac attttttact
    10021 aattttttca attcgacaac ttctttcttt tcaactacta ctaaaagtac atttttattt
    10081 tctccagtat atgcaccttc tccttttaga atagttgcac ctctatctaa atcttccatt
    10141 atttttttac ttatatcttt tgaaaggtca gatataatag tgaatgattt ggaataatta
    10201 aatccttcaa taagcaagtc actaactttt actaaaatgt ataaagccac agttgaataa
    10261 agtgcaattt caacctgtct atttactata cctgataaaa caactacaac gcagtctaca
    10321 atacccatta aaacaggtat actaaatgta ggaaaatatt tatttagtag gagtgccatt
    10381 aaatcagttc cacctgtaga gccatttatt ctaaaaataa gtccttgacc aactcccatt
    10441 aaaatagaac cagatataat tgcaagatac atatcatttg ttatatctaa tgtagccata
    10501 tttgcagtaa gcttaagtgc tatggtaaga aaaattattc ctaaaaccgt tttaaaacaa
    10561 tctttttttg aaagtatttt gtaagcaaat atgaataatg gaacatttaa aagtaaattg
    10621 acaatccaaa gttctacacc gaataatgaa tttaaaacaa ctccaagtcc tgttatacca
    10681 ccagcagcga ttgcatgtgg attaaaaaat aagttaagag aaatggccat taatatacag
    10741 cctacaatta atattaaaat ttctgaaaaa gttattctat cttttttcaa taacatcact
    10801 ccttatatat agattataat atatattata tagtttttcc aatttttaat aaaagaaata
    10861 aaaatatatt ataaaaacaa tttgattgga attttcagaa aattaatgct ataattaaaa
    10921 gtaaatttaa agggaggtta taaaatggct aaattaactc tagaacatat aaaaggaaat
    10981 acatattata ttccactccc tacaatagta ggtgtatacg tagatggtaa ggatgcaatt
    11041 ttaattgaca gtggaaataa taaggataca gctagacaag ttttaagatt attagaagaa
    11101 cataatttaa atcctaaatt aataataaac actcattcaa atgcagacca tataggagga
    11161 aatgcatatt tgaagaatca aacaaaatgc aagattgcta cgactaaaat tgaaggatat
    11221 tttacagaaa atccaatatt agaatcagca tttttatatg gtggctatcc aagtaaagca
    11281 ctgaagaata aatttttatt agcaaaggaa agtgaagttg attatattat cccttcaaat
    11341 ggcaaaattg tagatacaga acttgaagcg atttctcttc caggtcatta ttttgaaatg
    11401 attggtgtta gaactccaga taatgttttg tttgttgcag atagtcttac accagagaat
    11461 ataattacta aatatcattt tttcttccta ctggatatag attcacagtt taagacacta
    11521 gaaaaattga gaatgttaga ggctgatttc tttgttccaa gtcactctgt caaaactaca
    11581 gatataaaaa gtttaattaa tattaataag aagaagatgg aagaaataat agataatatt
    11641 aaaaaggttt gttatgagcc agttatgatt gatgaagtta ttgaaaaaat gtgtgatttg
    11701 tacaatgtca agcttgatgc taatcaatat gtgttggttg gaagtacaat acgttcttat
    11761 ataacttatt tatatgagaa taatatggtt gaatatatat ttgatggtgg taagatgatg
    11821 ataaaagctg tgtaaatagt aataattttc aaattatatg atgaaattgt tttagtatga
    11881 ctgttatttg aaaagtaata taaaaaaatt tatataaata agctaagatg attgatataa
    11941 agttactgat aattttaagg taatatatac gttactataa tattatgtta ttatttgtaa
    12001 ataaggggat aatttataag caaattaaag accttaattt acaggaggaa tagtgtatat
    12061 tatgaataaa aaattcaaaa aattcagtaa aaaactaagt atattaattt tagttttgat
    12121 tatgtttctt tttaataatt taactgcaaa tgcacagatt aaagaagatt caataatagg
    12181 aattgataga tatgaaacag caggacttat agcagataga caaacttatg atactgtaat
    12241 attagttaat ggagataaaa atttatctga tgggctttca tcaagtggat tagctggagc
    12301 tattaacgca ccaatacttt tgactaaaaa aagtgaaata ccaaaagcta ctttaaatag
    12361 actagataat aagagtttaa atgttgtaaa aaaagtatat ataattggtg gttataatac
    12421 tattgaaaat tcagtagaaa aagatataaa aagaaaaggt attcaagtgg aaagaataaa
    12481 tggtaacaat agaattgaaa ctagttataa tgtagctaaa aaaataaatg aagtaagtag
    12541 tgtaaaagag gtaatgctta caaatggatt tgtaggagag gctgatgcta tgagtatagc
    12601 acctgtagtt gcaaaaaata aaggagctat aatacttaca gatgggaaat cagttccatt
    12661 taagactgaa agtttaaatg tttacgctat aggtggcaaa tctgcgataa atgaggattt
    12721 agtaaatgaa actaaagcaa ctagaatcgg aggaaatgat agatttgaga ccaataaaaa
    12781 ggtaatggaa aagttttata atggagcgac agatttttat ataacaaatg gatatcaatt
    12841 agtagatgca ctaacattat ctcctttagc caaagaaaaa ccaatcgttt tagttgctga
    12901 tggaagtaat aaaggtattt taaaaggagc taaaagtata acaaaagttg gaggtattga
    12961 tgccaatata tataaacagt gtttagatat gattaaatat aataatatgg atatgactcc
    13021 aaatatttta aaacacttaa cttcagttga aggaaatgaa gtttctgaaa tacttataga
    13081 atcagataat ttaggtgtgg ttgttgaaaa gacgaatacc aataaatttg agtttgatta
    13141 cgtaagtgta actaatgaaa aaaattgtac tttttctgta tataaagaaa attcttctaa
    13201 taatataaaa aatggaaagc tagttgtaag tgctaagaaa aaaatagata agaaagatag
    13261 tgatttgcaa gatatgaatg gcgacaatat gataaatgca aataaagata ggatggtaaa
    13321 tgtgataaaa attggagttc ccgataaaat gtattctaat tttaaagtag atgttgaaag
    13381 tggttcagta gaactttata atattaaagg aggagctact gttgatgtca atgacgggat
    13441 tgctaacatt gttgataaca atgtaacaaa tcctttcaat ataaatacta atagtgggat
    13501 ttcaggtgta acagctgaaa ctatatcatc tgaaataaaa tttagagcaa acaatggaat
    13561 tgtaaatgta acagctacga atataaatgg caatataagt ttatttggaa gcgataataa
    13621 aggtaccttt gatggaatat ttaaattaaa tctgaaaaaa gaaccatcaa atttacactt
    13681 gaagttaatt ggaaatggga taaataaatt gccagaaggt tggtctaaag attatatttt
    13741 aggaaatgga caaccaatta ttgaagttaa aaataatggc ataaataata tatcaattgg
    13801 agaataaaaa catatgaaaa taaagtttat atagttttaa aaattataga attttaaaat
    13861 atttatatat attctttaaa taaaaaatta t
//
LOCUS       DAYAPA010000118        13547 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.120, whole genome shotgun sequence.
ACCESSION   DAYAPA010000118 DAYAPA010000000
VERSION     DAYAPA010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13547
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.120"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(379..2046)
                     /locus_tag="ACGI59_003063"
     CDS             complement(379..2046)
                     /locus_tag="ACGI59_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HFG9740841.1"
                     /translation="MKKINLKIEGKDKEYSLEENSPGIRLGDIAKEFCDEHKGYITLA
                     VVDNKLKELNCRVKKDCEINFLDTTNEDGERVYFRVMSFIFVMACREIFWDSRVTIEH
                     SLSDGLYCEVHIDRKLKEADVENIKNKMKEIVNNDYVIEKIEVTRNEAISIFENNEMY
                     EKAELLKYKEYDSAKVYKCRNYIDHFYGYMLPSTGYIKSFDIKLYNGGVIILGPSEED
                     KTLPMKFVPQPKLSSVYYEAEEWSRLMGIDKVVSLNKIIENNEYGDIIRTFEALHEKK
                     LSQIADMIKDGNKRVVLIAAPSSSGKTSFAHRLSIHLRVNNLNPISISLDDYFIDRKH
                     TPLDEYGNYDFESIYAIDLEKFNLDLKKLLAGEEIDDIRFNFKEGKREYTGKKIKIDS
                     NQPIILEGIHGLNPILTSSIPDEDKFKIYISALTQINLDDHNRIPTADLRMIRRIVRD
                     YNFRGYSADNTILQWASVRRGEKKNIFPYQEEADAIFNSACVYELAVLKRFAKPLLEE
                     IKEDNPAHIEATRLLKFLQYFVELDDTSDIPGISILREFIGGSKIVD"
     gene            complement(2120..2875)
                     /locus_tag="ACGI59_003064"
     CDS             complement(2120..2875)
                     /locus_tag="ACGI59_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HFG9740842.1"
                     /translation="MAVGIASLLFNIEEALNICESIKQITHLEIGIDNISECSELCKY
                     KERISKLGLSIGIHLPMELNTCENIEYIRNSWINFIEKIEFELKGFDLRYFNLHLGYV
                     MTNRVRKNRDKYLGNSVDFLDKLNTNSYVCIENTYSKGGDFSNIGNISYDFEYIFKRI
                     KNSKICFCYDTGHCLIDEDAYVKNLKDKIRLIHLSDNDGINDTHVGIGRGILSEEGIK
                     EILTLDAEYLVLEINYEDIEDTISKLNNIVGEG"
     gene            complement(2885..3421)
                     /gene="lepB"
                     /locus_tag="ACGI59_003065"
     CDS             complement(2885..3421)
                     /gene="lepB"
                     /locus_tag="ACGI59_003065"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFG9740843.1"
                     /translation="MNETIKEEIVEWIKIIITALFFAFIITRFIKPTLVNGESMYPTL
                     KSHDYLVANRMTYKLSEPKCGDIMIFKTDLLQENGRKKELVKRVIGVPGDHLKIKDSK
                     VYINGKLLNEVSYIHDNYTEGDIDMVIPKGKVFAMGDNREVSLDSRYKEVGLVDEENI
                     KGKVILRVFPFTDIGIFE"
     gene            complement(3587..4123)
                     /gene="lepB"
                     /locus_tag="ACGI59_003066"
     CDS             complement(3587..4123)
                     /gene="lepB"
                     /locus_tag="ACGI59_003066"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFG9740844.1"
                     /translation="MGEAVKKEVVEWIKVIVIALVLAFAITRFIVPTIVKGESMYPTL
                     VERDYLIVNRIAYKVGEPKYKDIIVFKTDLTEENGKKKDLVKRVIGVPGDHVKIQDSK
                     VYVNDKLLDETSYIHNNRTDGDIDIVVPEGKLFAMGDNREKSLDSRYDEVGLVDEHTI
                     LGKVLVRLYPFSKIGTID"
     gene            complement(4223..5533)
                     /locus_tag="ACGI59_003067"
     CDS             complement(4223..5533)
                     /locus_tag="ACGI59_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HFG9740845.1"
                     /translation="MTELPKEFLEDIEEILKDEYDDFIKSYEESKTTGLRINTLKIDK
                     KKLFDLELFDLNQIPWAEEGFYYDDKIDRPGRNPLHEAGAYYLQEPSAMSVIPKADIK
                     EGEKVLDMCAAPGGKSTYILSKLNDTGLLVSNEINASRIKALGENLERFGARNCIITN
                     TDSNSLRKTFNGYFDKVIIDAPCSGQGMFRKDEVAISDWSYAKVVECQSIQKEIIRDG
                     YKMLKKDGILIYSTCTFSREENEHVINEFISEYPHAELIEMERLWPHKIKGEGHFVAK
                     IKKLEEEECNVKELKIKNLDKEIKDYREFEKKFLKVDFYSNKKNKFYLRGENLYLLPE
                     IYPDTKKLKVLRYGLHLGVLKKNRFEPSHALSHYLKVEDVKNVENFSVQSESILKYLK
                     GDVVNSNDSRGWVLVSVEGIPLGWGKESSGVIKNHYPKGLRKVF"
     gene            complement(5612..6328)
                     /locus_tag="ACGI59_003068"
     CDS             complement(5612..6328)
                     /locus_tag="ACGI59_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HFG9740846.1"
                     /translation="MKLDLFLLAIIPILIGMFWIRSKDRYCREPLIHLIKFFLIGAFL
                     SVIIILLENLLMKFNVFEGYSELIYVSFVVAGLVEEGVKALILIPALIKEKHFTEKLD
                     GIIYSVFLALGFATIENMVYIFSESRNLALQVGINRAVISIPAHVMFAITMGYYISKY
                     KFEGNKNKRREYLFMAVLIPILLHGVFDFILMIEYRWAIILLIVYVIILWKINLDKLE
                     KYMNHSKKVFFGNLRKKKKK"
     gene            6573..7844
                     /gene="sleC"
                     /locus_tag="ACGI59_003069"
     CDS             6573..7844
                     /gene="sleC"
                     /locus_tag="ACGI59_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex-lytic germination protein SleC"
                     /protein_id="HFG9740847.1"
                     /translation="MQDGFLTVSIIDATNNRPIQNAVVNIYSMSNGSQSSSTLYQNLR
                     SNESGQVTGLVLPAPDVDYSLQPSDVRPYSQYIVEAIADGYETVVIEGTQLLATIEAR
                     QGVPMSPRTRSKRSFSRQSELIFDIGEHTLYGTYPPKIPESNLKPLPPPTGFVVLDNP
                     VVPEFIVVHDGLPEDSSAPNYWIPFKEYIKNIASSEIYSTWPEQTIYANVIAIISFTL
                     NRVFTEWYRNKGYNFTITSTTAYDHKFINNRNLFEPINVVVDAIFNTFIKRPPTSRQP
                     LLAQYCDGQKSQCPDQMTQWGSKDLGDQGYDYESILRYFYGDEIVFERAPIVSGVPVS
                     FPGTTLQVGSSGQYVRTIQNQLNAISNSYPAVPKVIEDGIYGTDTENAVKIFQGIFGL
                     PQSGVVDFKTWYEISRVYVATTRIASLNPLI"
     gene            complement(8270..8926)
                     /locus_tag="ACGI59_003070"
     CDS             complement(8270..8926)
                     /locus_tag="ACGI59_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9740848.1"
                     /translation="MKKGDFIWSGVLICIIAVLAIPSSREVFMEATGAHPYIGGFLKF
                     AVLATMGDLLGVRILYKDWKIPVGVFYRAIVWGIIGVVTTYGMSIYSSGVGQAQVDGM
                     LPFEGIAFANALFKSATMNILMSPTVFLFHKVMDCFIDKKYEVGKGIKVKLKDVVNIV
                     DWNAYLGFSVLKTIPFFWIPCHTIVFLFPAEYRVIASAFASIALGLILALANKSKVAV
                     "
     gene            complement(10038..10448)
                     /locus_tag="ACGI59_003071"
     CDS             complement(10038..10448)
                     /locus_tag="ACGI59_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9740849.1"
                     /translation="MKIKKSLISAMMVSAMVLGGTGAVFANGNDSSNIALPKFESLIK
                     SGITDPTELEKYGVEHYTYEEYAKHIQELKDYAKDPNAVKDVSQKDLEETIKKMEQEL
                     EKIKTEGLKIMKPITIENEDGSQISIAYNYAAID"
     gene            complement(10664..12046)
                     /locus_tag="ACGI59_003072"
     CDS             complement(10664..12046)
                     /locus_tag="ACGI59_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HFG9740850.1"
                     /translation="MFIKLLEMSFTSGVLLLIVIFIRACLFKKLPKKVFVLLWLIVLF
                     KLLIPVSLNSGFSIYSIIESTFDVELGNTSTENYTLKKASDSTGKNNSDEFEIMSNIE
                     NIMMLEMLGILSFIVTIMFSILLCFRYFKIINLFKTSIHIKNHEFIKKWMCKNSRFRR
                     FIVCISEFTRTPLTSGILKPRIILPKQMNYANNQTLDFVLTHEMVHINSFDNLLKLFM
                     LVALVVHWFNPLVWVMFFLLNKDIEFSCDEKVMSILGEEYRADYATTLISLAEENNPF
                     SMTICSGFGKTKIEKRIVNIMKFKKSTYVTFLVSTVLVCGTGLVFATENKEINEVESF
                     GLTKNEQSDKIGFSISSKSTDLYSPKVESDILVSKAKSDGETHFSTAKVSVIEFREHE
                     ESSMSKVTNSENSEETYVNKSSNSKDVLYTDSGEVSIERSGYNDNRFVILRKGNLLAV
                     NKEQYRTWIE"
     gene            complement(12057..12416)
                     /locus_tag="ACGI59_003073"
     CDS             complement(12057..12416)
                     /locus_tag="ACGI59_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HFG9740851.1"
                     /translation="MEIKLFDSELKVMEVLWERGESKAGELVKILKEETGWNRNTTYT
                     VIKKLVSKGAIERIEPNFVCKALISKEEVQKQETETLINKMFGGSSEKFLSAFINKEN
                     LSSDEITKLKQIVEKLK"
     gene            complement(12621..13400)
                     /locus_tag="ACGI59_003074"
     CDS             complement(12621..13400)
                     /locus_tag="ACGI59_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFG9740852.1"
                     /translation="MVSRQELKRVSKAQLSGNWGICAISMAVYIALIVLISIVGLPFE
                     PLTTIVVAVIAFCVSAGFTCMFLKITKDEKVKIGDIFVSGRIYLRSFGFYLFESIIVY
                     IYSFIITIIGAFLSFGVLLTSGAIEYLSSDNVGIGTIISTGITLVLIILVLMLPVYIL
                     LLYVSQATFLICEDKDNMGVFKSMGASLDMMKGNVLKLFVLNFSFIGWYILSIATLGI
                     GLLWLIPYVGVTNCNFYRQLVKENPDVEDILLDKEKSYTMY"
BASE COUNT         4608 a         2455 c         1330 g         5154 t
ORIGIN      
        1 acaacctagt aatttagcaa tccagcaatc taataatcca gtaatttaaa tttttataac
       61 accaaaaatt tctaacccaa caccttttac tcttttactc ttttactctt ttactctttt
      121 actcttttac tcttttactc ttttactctt ttaaaaaata atcacataaa ttaacaaaat
      181 attaaacaaa aaagtaaaat ttaaaataaa aagcttgctt catacaaaag tataaaacaa
      241 gcctttttat atatcaactc aaactttaaa atctgtatca atttaattct ccactttaca
      301 cttaatatac ttaacataaa ccaataacac tacttgctat gccatttaat cttcataaaa
      361 caatctataa tacacacatt aatcaactat tttacttcct ccaataaatt ccctaagaat
      421 tgaaatacct ggtatgtcag aagtatcgtc caattcaaca aaatactgca aaaactttag
      481 taatctagta gcttcaatat gtgcaggatt atcttccttt atctcttcca acaaaggttt
      541 cgcaaatcgt tttaaaactg ccaattcata tacacaagca gagttaaata tagcatctgc
      601 ctcctcttgg tacgggaata tattcttctt ttctcctctt ctaactgatg cccattgtaa
      661 aatagtatta tcagcactat atcctctaaa attatagtct ctaacaattc ttcttatcat
      721 tctcaaatca gcagtaggga ttcgattatg gtcatccaaa tttatttgag taagggcact
      781 tatgtatatc ttaaatttat cttcatctgg tatagatgat gttaaaatag gatttagccc
      841 atgtatacct tctaatataa ttggctgatt tgaatctatc tttatctttt taccagtgta
      901 ttctctctta ccttctttaa aattgaatct tatatcatca atttcttcac cagctaaaag
      961 tttctttaag tctaagttaa acttctctaa atctattgca taaattgatt caaaatcata
     1021 atttccatat tcatctaaag gagtatgttt tctatctata aaataatcat ctaaagaaat
     1081 tgaaatagga tttaaattat ttactctaag atgtattgat aatctgtgag caaaacttgt
     1141 ttttccagat gaagatggag ctgctattaa aactactctc ttattgccat cctttatcat
     1201 atccgctatt tgggataact tcttttcatg caatgcttca aatgtcctta ttatatctcc
     1261 atattcattg ttttctatta ttttattaag agatactacc ttgtcaattc ccataagtct
     1321 tgaccattct tctgcttcat aatatacact tgatagctta ggttgaggta caaatttcat
     1381 aggaagagtc ttatcttctt cactaggtcc taaaattata acgcctccat tataaagctt
     1441 aatatcaaat gattttatat aaccagttga tggcaacata taaccataaa aatggtctat
     1501 atagtttcta cacttatata ctttagcact atcatactct ttgtatttca aaagctctgc
     1561 cttttcatac atttcattat tttcaaagat agaaatagct tcatttcttg taacttcaat
     1621 tttttctata acataatcgt tgtttactat ctctttcatc ttgtttttta tattttcaac
     1681 atctgcttct ttaagctttc tatctatatg aacttcacaa tatagcccat ctgatagtga
     1741 atgttctatt gtaactctgc tatcccaaaa gatttctcta catgccatta caaatataaa
     1801 tgacatgact ctaaagtaaa ctctctctcc atcttcatta gtagtatcta aaaaatttat
     1861 ctcacaatcc ttcttaactc tacaatttag ctcttttagt ttattgtcta caactgcaag
     1921 tgttatataa cccttatgct catcacaaaa ttcttttgct atatctccca atctgattcc
     1981 aggggaattt tcttctaatg aatattcctt atcttttcct tctattttta agtttatttt
     2041 tttcacttca acatctcctt ccttttgatg aatataaatt ttaattaatg aggcatatca
     2101 acaagataaa gagaggtatt taaccctctc ctacaatatt attcaattta cttatagtat
     2161 cttcaatgtc ttcgtaattt atttctaata ctaaatattc tgcatctaat gttaagatct
     2221 ctttaatccc ctcttcagat aaaattcctc ttcctatacc aacatgtgta tcattgatac
     2281 catcattatc acttaagtgt atcagtctta ttttatcctt taagttcttt acataagcat
     2341 cctcatcaat taaacagtgt ccagtatcgt aacaaaaaca aatcttagaa tttttaatcc
     2401 tcttaaatat atattcaaaa tcataagata tatttccaat atttgaaaaa tccccacctt
     2461 ttgaataagt attctctata catacatatg aatttgtatt taatttatct aaaaaatcca
     2521 cactatttcc taaatatttg tctctattct ttctaactct atttgtcata acatatccca
     2581 aatgaaggtt aaaatacctt aaatcaaatc cttttagttc aaattctatc ttctcaataa
     2641 aatttatcca tgaatttcgt atatactcta tgttttcgca agtatttaat tccataggca
     2701 aatgtatccc tatagataat cctaacttag aaattctctc tttatattta cataactcac
     2761 tacattcaga tatattgtca attccaatct ctaaatgagt tatttgcttt atactctcac
     2821 aaatgtttaa agcctcctca atattaaaca ataaggacgc tataccaact gccatacaaa
     2881 tcacctactc aaaaatacct atatctgtaa aaggaaatac tcttaaaata acttttcctt
     2941 taatattttc ttcatctact aatcccactt ctttatatct actgtctaaa ctaacttctc
     3001 tattgtctcc catcgcaaat acttttccct taggaatcac catatcaata tcgccttcag
     3061 tataattatc atgtatatat gaaacttcat ttaataactt accatttata taaaccttag
     3121 aatccttaat ttttagatgg tcaccaggaa cacctataac cctttttaca agctcttttt
     3181 tccttccatt ctcttgtaat aaatcagtct taaatatcat tatatctcca cattttggtt
     3241 ctgataactt atatgtcatc ctgtttgcta ccaaataatc atgtgattta agtgttgggt
     3301 acattgattc tccatttact aatgttggtt ttataaaacg agttataata aatgcaaaaa
     3361 aaagtgcagt aataattatt tttatccact ctacaatctc ttctttaata gtttcattca
     3421 ttcacaaatt ctcctttata gttatattat ctttattgat aatgtctctt aaacactatc
     3481 ttttagataa gtctactaaa taaggtgttg tcttttttat atagacaact attgtgtatg
     3541 gtctggtgat ttcagctaat cagcgattgc tccaaatttt atatgtttag tcaatagttc
     3601 ctatcttaga aaatggatac aatctgacta gaacctttcc taaaatggta tgctcgtcga
     3661 ccaatccaac ctcatcgtat ctactatcta aacttttttc tctattatct cccattgcaa
     3721 ataattttcc ttctggaact acgatatcaa tatctccatc agtacgatta ttatgtatat
     3781 aggaagtctc atctaacaac ttatcattta catatacctt ggagtcttgt atttttacat
     3841 ggtcaccagg aaccccgata actcttttta ctaaatcttt tttctttcca ttttcctctg
     3901 ttaagtcggt tttgaatact attatatctt tgtattttgg ctctcctacc ttgtacgcaa
     3961 ttctgttaac tatcaaataa tcacgttcaa ctaatgtagg atacattgat tctcctttga
     4021 ctattgttgg cactataaaa cgagttattg caaatgccaa aacaagagct atgacaatca
     4081 cttttatcca ttctacaact tcttttttaa ctgcttcacc cactaaaatc gtctcctctg
     4141 tgttaaatat taagtcaatc tacagcaaaa taatctacca taaacctaca atataaatat
     4201 tatacttctt ttttctaata gtttaaaata cttttctaag acctttagga taatgattct
     4261 taattacacc agaagattct tttccccaac ctaaaggtat tccttcaacc gaaaccaaaa
     4321 cccatcctct actatcatta gaatttacaa catccccttt taaatattta agaattgatt
     4381 cactctgtac actgaagttt tcaacatttt ttacatcttc aacttttaag taatgagata
     4441 gtgcatgaga aggctcaaat ctattcttct ttaaaacccc taaatgcaaa ccatatctca
     4501 atacttttag ttttttcgta tctggatata tttcaggaag taggtacaaa ttttctcctc
     4561 ttaaataaaa tttatttttt ttattactat aaaaatcaac ctttaaaaat ttcttttcaa
     4621 actccctata atcctttatt tctttatcta aattttttat ttttaattct ttgacattac
     4681 attcttcttc ttctaatttt tttattttgg caacaaaatg accttctccc tttatcttat
     4741 gaggccataa tctttccatt tcaataagtt ctgcatgtgg atattcactg ataaactcat
     4801 ttataacatg ctcattttcc tcccttgaaa aagtacaagt tgagtaaatt aaaatgccat
     4861 ccttctttaa cattttatat ccatctctta taatctcttt ttggatggat tgacattcta
     4921 ctacctttgc ataactccaa tctgatatgg caacctcatc cttcctaaac ataccttgcc
     4981 cagaacaagg tgcatctata ataaccttat caaaataccc attaaacgtc tttcttaagc
     5041 tattagaatc ggtgtttgtt ataatacaat ttctcgctcc aaatcgctct aaattttccc
     5101 ctagagcctt aattctacta gcattaattt cattagaaac caataaacct gtatcgttta
     5161 gcttagatag tatatatgta gattttccac caggagcagc acacatatcg agaacttttt
     5221 caccctcttt aatatctgcc tttggtatta cactcattgc agaaggctct tgcaaatagt
     5281 atgctccagc ctcatgcagt ggatttctac caggtctgtc aattttatca tcataataaa
     5341 aaccttcttc tgcccatggt atttgattca aatcaaacaa ctccaaatca aataattttt
     5401 tcttgtcaat ttttaaagta tttattctaa gcccagtagt cttactttcc tcataacttt
     5461 ttataaaatc atcatattca tcttttagta tttcttcaat atcttctaaa aattcctttg
     5521 gtaattctgt caaatctata atcccccttc aatctgcttt acttgtgtgt catttattat
     5581 actttaatta tttatgtctt tcctttaaat tttatttctt cttctttttc ctaagattac
     5641 cgaaaaacac ttttttagag tgattcatat atttctcaag tttatcaaga tttatcttcc
     5701 acaaaattat tacataaact ataagtaata taatagccca tctatattct atcatcaata
     5761 tgaaatcaaa aactccatgt aataaaattg gaattaacac cgccataaac aaatactcac
     5821 gtcttttatt tttatttccc tcaaatttat acttagaaat atagtagccc atagtaattg
     5881 caaacattac atgagctggt atagagataa ctgccctatt aataccaact tgcagtgcta
     5941 gatttctact ttcagaaaaa atataaacca tattttcaat tgttgcaaat cctaaagcta
     6001 aaaatacaga gtaaataata ccatctaact tttcagtaaa gtgtttttct ttaattaggg
     6061 caggaatcag tatcagagct tttacacctt cttctacaag tcctgcaact acaaatgata
     6121 catatattag ttctgaatac ccttcaaata cattaaactt catcagtaaa ttttctaaaa
     6181 gtataattat aacacttaga aaagcaccta ttaaaaaaaa tttaattaga tggattaggg
     6241 gttctctaca atatctatct ttagacctta tccaaaacat ccctatcaat ataggaatta
     6301 ttgctaataa aaataaatct agcttcatag attattttcc tttcaaaatt tttgatttat
     6361 ttatgattta tatcatctac ttatttttaa cttattcgtg ttttaaatac atactaaaat
     6421 atacattaaa ttattttaaa ttatttaaat acaaatatac tcaaatactc aaaaaaattt
     6481 aaatttatat ctatattatt taacaaattt caagctatat tcatatcttg aaatataagg
     6541 gaactaaatt catttaaaga aagggtgatt tgatgcaaga tggtttctta acagtaagca
     6601 taattgatgc tactaacaat agacctattc aaaatgcagt agtaaacata tattcaatgt
     6661 ctaatggtag ccaaagctca tcaactttat accaaaacct aagaagtaat gaatctggtc
     6721 aagtgactgg tttagtatta ccagcaccag atgtagatta ctctctacaa ccatctgatg
     6781 ttagacctta tagccagtat atagtggaag ccatagcaga tggatatgag acagttgtca
     6841 tagaaggaac acagttgtta gctacaattg aagcaagaca aggagttccc atgtctccta
     6901 gaacaagaag taagcgttct ttctcaagac aaagtgagtt gatttttgat ataggagaac
     6961 atactctata tggtacttat cctcctaaga ttccagaaag taacttaaaa ccattaccac
     7021 ctcctactgg tttcgttgta ttagataacc ccgtagtgcc tgaatttata gtagttcatg
     7081 atggtttgcc agaagattca tcagctccaa actattggat tccatttaaa gagtacatta
     7141 aaaacatagc gtcatctgaa atatattcaa cttggcctga acagacaatc tatgcaaatg
     7201 taatagctat aatatcattt actttaaata gagtctttac tgaatggtat agaaataaag
     7261 gatataactt tacaataact tcaacaacag catacgacca taagtttata aataatagaa
     7321 acttatttga accaattaac gtagttgtag atgctatttt taatactttt ataaaaagac
     7381 ctcctacatc aagacagcca ctattagcac aatattgtga tggacaaaaa tcccaatgtc
     7441 ctgaccaaat gactcaatgg ggcagtaaag acttaggtga ccaaggatat gactatgaga
     7501 gtatactaag atatttctat ggagatgaga tagtatttga aagagcacct atagtaagtg
     7561 gtgtaccagt gtcattccct ggtactacac tacaagttgg ttcttctggg cagtatgtta
     7621 gaaccataca aaatcagtta aatgctatat ctaattctta tccagctgta ccaaaagtta
     7681 tagaagatgg tatctatgga acagatacag aaaacgctgt taagatattc caaggaatct
     7741 ttggtttacc tcaaagcgga gtagtagatt ttaaaacatg gtatgaaata tccagagttt
     7801 atgtagctac aactagaata gcttctttaa atcctttaat ttaaagcttg atttagaaca
     7861 taaactcaaa ttattgaata aaattattaa tttaaaataa aagtggctgt tatgaactaa
     7921 tatataccat aagtattact tataagtatt gtagtcttaa cagccatttt ttagttataa
     7981 cttgtttttt tagaatctta aaactttaaa tgggggattt taagcttctt aaaaacagtg
     8041 cttagcaact tagatttctt taaaatatta tatttatttt taagtttaat aaaatgctat
     8101 tctaacgaaa aacctacttt ttatttaaaa tctcacttat tatttaaaac actatccatc
     8161 acttagaata ttacttctta tataaattat tatttattat aaatacaaat aatattaatt
     8221 taaaaagtta ttcctaattt aaataatact actatcttca tcagtgaacc taaactgcaa
     8281 ctttagactt attagctaaa gccaatatta gacctaaagc tatagatgca aatgcagaag
     8341 caattactct atactcagca gggaataaaa atacaattgt atgacatggt atccagaaaa
     8401 atggtatagt ttttaaaaca ctaaaaccaa ggtaagcatt ccagtccaca atatttacca
     8461 catctttaag ttttacctta atcccctttc caacctcata ttttttatct ataaaacagt
     8521 ccataacctt atggaataaa aatacagttg gtgacattag tatattcata gttgcactct
     8581 taaatagagc atttgcaaaa gctattccct caaatggaag cataccatcc acttgtgctt
     8641 gacctacacc tgatgagtag attgacattc catatgtggt aactacacct attattcccc
     8701 aaactattgc tctataaaat actcctactg gtattttcca atccttatac aaaattctaa
     8761 caccaagtaa atctcccatt gtagctaata cagcaaactt taaaaaacct ccaatatatg
     8821 gatgtgcacc tgttgcttcc ataaatactt ctctactact tggtattgct aagacagcaa
     8881 taatacaaat aagaactcca ctccatataa aatcgccttt tttcatattt ataaatacta
     8941 tttaatatat aaatagttct cctttctatt aaagatttat ttaatttaca atctatctac
     9001 aattatttat taatcactta ttattagata ttcattttcc atatttaaat ttattctatc
     9061 cctttatcta tttattttct tactactcta tccaccatta tctatatatt tattttacga
     9121 ttactctttc accctaaccc tttaactatc tattttttct tactattctt atccacatca
     9181 tccatatatt tattttacga ttactctttc actctaaccc tttaactatc tattttttct
     9241 gatattctta tccaccatca tccatatatt tattttacga ttactctttc accctaaccc
     9301 tttaactatc tatttttttc tggtattctt atctactatt atctgtatat ttattttatg
     9361 attactcttt taacctaccc cctatacttt atagtaatat gtttttaacc tttttcatta
     9421 ttcccaatat gctttataac tacaaatact ctcccccctt aaaaataata ataaaattaa
     9481 tcaataaatt tataatcata ttattactct atgtattaag caattactat gccaataaac
     9541 tttgattttt ttaaacaaat cttaaaaatc ctaaaatgct aattttcttt aacttttaat
     9601 gaatctaata gataacattt aattttcgtt atatttttca gaatatttct attattattt
     9661 ttaagaaaaa aaaataatgt taacatttct taacatacat taaaaaatgt taacattata
     9721 aattgggttt aatactttta ctttgactcg atttactttt attctacttc ttactttatt
     9781 tttaacttag actttatttt tttattactt atttgagtct tatttatttt attctcactc
     9841 catatgttca attacatttg tctaaattct ttgatacttt tattacataa ttttaacaag
     9901 cttaagtgtt atttgggtag tgcagttgat aattatttaa ataatcatca actgcatgtt
     9961 tatatcatta cagtagctta atgtatttaa tcatacttac ttaggatttt gaaatatctt
    10021 agagattata tttaacatta gtcaattgct gcataattgt aagcaattga aatttgagac
    10081 ccatcttcat tttctatagt gataggcttc ataattttta atccttcagt ttttatcttt
    10141 tctaattctt gttccatttt cttaattgtt tcctcaaggt ctttttgact tacatctttg
    10201 actgcattag ggtctttagc atagtctttt aattcttgaa tatgtttagc atactcttca
    10261 taagtataat gctcaacacc atacttttcc aattcagtag ggtcagttat tcctgatttt
    10321 attagagatt caaatttagg taaagcaata ttagatgaat catttccatt tgcaaatact
    10381 gctccagttc ctcctagtac cattgcagat accatcattg cacttataag ggattttttt
    10441 attttcataa ccaatcttcc tttcttagag atttatttgg gtagtgtagt ttaaaatgac
    10501 tattgcaata gttatcaact acactcttag actatcacag tagtcatgtt tatgtcaagc
    10561 ctttttaaaa aacttttcaa taaaaaatac tatctcaaag caaaattaat tattttgaga
    10621 tagtatttta attctaaatc ttttatatta ctaagattta tggttattct atccatgttc
    10681 tatattgttc tttattaact gctaacaaat tcccttttct taatatgaca aagcgattat
    10741 cattatagcc acttcgctca attgaaactt ctcctgaatc agtataaagt acatccttac
    10801 tattactaga tttatttaca tacgtttctt cactattctc agaattagtt actttgctca
    10861 tagatgattc ttcatgctct ctaaattcaa ttacagatac ttttgccgta ctaaaatgag
    10921 tctctccatc acttttagct ttactaacaa gaatatcaga ttctactttg ggagaatata
    10981 aatcagttga tttactactt attgaaaatc caattttatc actttgttca ttttttgtta
    11041 aaccaaaaga ttcaacctca ttaatctctt tattttctgt tgcaaaaaca agacctgtcc
    11101 cacaaactaa tacagttgaa actaaaaaag taacataagt tgatttttta aatttcataa
    11161 tattaactat tctcttttct atttttgtct ttccaaatcc actacatatt gtcattgaaa
    11221 atggattatt ctcctctgct agacttatta gtgtggttgc ataatcagcc ctatattctt
    11281 caccaagtat actcataacc ttttcatcac aactaaattc tatgtcttta tttagtaaaa
    11341 aaaacatcac ccaaactaag ggattaaacc aatggacaac aagagctaca agcataaata
    11401 actttaacag attatcaaag ctattaatat gaaccatttc atgagttaaa acaaagtcta
    11461 aagtttgatt attagcataa ttcatttgct ttggcaaaat aattcgtggt tttaaaatac
    11521 cagatgtaag tggtgtccta gtaaattcag atatgcaaac aataaatctt ctaaaacggc
    11581 tattcttaca catccatttt ttaatgaact catgattttt tatatgaatt gaagttttaa
    11641 aaagattaat tattttaaaa tatctaaagc ataaaagtat gctaaacata atagtaacta
    11701 taaaagacaa aatgcccaac atttccaaca tcataatatt ttcaatatta gacattattt
    11761 caaattcgtc agaattgttt ttacctgtag agtcactagc tttcttaaga gtataatttt
    11821 ctgttgatgt attacccaat tccacatcaa aagtcgattc aattatacta taaatactaa
    11881 aaccagaatt taaacttact ggtattagaa gtttaaataa aacaatcaac cacaataata
    11941 caaatacctt tttaggtaat tttttaaata aacatgctct tataaaaata actattaaaa
    12001 gtaatactcc agaagtaaaa ctcatttcta acaatttaat gaacataaat cttacctcac
    12061 tttaattttt caacaatttg tttaagtttt gtaatctcat cacttgaaag attttcttta
    12121 ttaataaaag ctgaaagaaa tttttctgaa gaccctccaa acattttatt aattaaagtt
    12181 tctgtttcct gtttttgtac ttcctctttg cttattaaag ctttacaaac aaaattcggc
    12241 tcaattctct ctattgcacc ttttgacact aattttttta taacggtata cgtagtgttt
    12301 ctattccatc ctgtttcttc ttttaaaatt tttactaact ctccagcctt gctttctcct
    12361 ctctcccata gaacttccat tactttaagt tctgaatcaa ataattttat ctccatataa
    12421 aatcgctcct caaatatagt tatcacaata gtcattacta taataaaaaa ctatcatagt
    12481 agtctatact atgtcaatca tttatttttt aataataaaa actgtctttt taaaaaattc
    12541 ttataaaaga cagttttatc aatgttatac tttaatgttt aattaaagcc atattttatt
    12601 taaatatata actatctatc ttagtacata gtataagatt tttctttatc taatagtata
    12661 tcctctacat ctgggttttc ttttactaat tgtctataaa aattacaatt agtaacaccc
    12721 acatatggaa ttagccatag tagacctatt ccaagagtag ctattgaaag tatataccat
    12781 cctataaagc taaagtttag tacaaataat ttaagtacat ttcctttcat catatctaaa
    12841 ctagcaccca ttgatttaaa tacacccata ttatccttgt cttcacaaat taagaaagta
    12901 gcttgagaaa catataatag caatatataa actggtaaca taagaacaag tattataaga
    12961 actagagtta ttccagtaga aataatagtc cctattccaa cattatcact tgataaatat
    13021 tcaattgctc cactagtaag taaaactcca aatgataaaa acgctcctat aattgtgatt
    13081 ataaaagaat aaatatacac aataattgat tcaaataaat aaaaaccaaa acttcttaaa
    13141 taaattctcc cacttacaaa gatatctcct atcttgactt tttcatcttt agtaattttc
    13201 aagaacatac atgtaaatcc tgctgaaacg caaaaagcta ttacagccac tacaatagta
    13261 gtcaatggtt caaaaggtaa tcctacaatt gatattaata ctatcaatgc aatatatact
    13321 gccatagaaa ttgcacatat cccccagttt ccagataact gagctttaga tactcgtttt
    13381 aattcttgcc ttgataccat aaaaaacacc cctttcattt tataaaaaaa cttttaaaaa
    13441 tataaataat cttttaacgt ataatattaa tatatcatat tttcgactat ataagataca
    13501 attattcacc atgttttatc aagatatgcc ataattgtat tactagt
//
LOCUS       DAYAPA010000119        13524 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.121, whole genome shotgun sequence.
ACCESSION   DAYAPA010000119 DAYAPA010000000
VERSION     DAYAPA010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13524
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.121"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            319..2178
                     /locus_tag="ACGI59_003075"
     CDS             319..2178
                     /locus_tag="ACGI59_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HFG9740853.1"
                     /translation="MKRFLRRIILVLFILLIFISFISIKLIHNVGDYGKLINYVGIVR
                     GASQRLTKLEMNHQPNDELIEYIDEILQELITGHGDYGLVITDCNEYNEDLLLLEKKW
                     EDLNIEIKKVRMKEQNNQLLSISEEFFSLANDTVFKIENFSKEKSNYLMTLIIIISII
                     GILACIILILQYSKKMVRLEKLNVDLKNIAYKDELTGVNTIEKFKLDANQNICIHKDK
                     KFAVFYIDFENFKYINDIFGYDYGDMILKRYANLMMNDIGKYEIFAREIADRFVALRC
                     YVDKEDLVVRQRDVDNELINITNEIKNKHSITVVSGICCIEDVNEKLSIDGLINRANF
                     AQKTVKNKPGTNYAFYNDSIRKKMIEENTIKSRIHEAIEKREFIVYLQPKVNLHSQKI
                     NCAEALVRWLTPDKGIISPAIFIPVLEKNFFIALVDKYVFEEVCKWIRKRLDENKPFV
                     QISVNVSRIQFYNTKFVETYSNIQNKYRIPKNTIEIEFTESVAFENQKHLLEIIHDLH
                     ENGFTCSLDDFGKGYSSLSVLKDLPFDVLKLDSMFFKASLDKDKEKIVIKNIVHMLKE
                     LNITTVAEGIECKEQVEFLRDIGCDLVQGFVFYKPMPILEFEKILDKEFVYNS"
     gene            complement(2470..3069)
                     /locus_tag="ACGI59_003076"
     CDS             complement(2470..3069)
                     /locus_tag="ACGI59_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFG9740854.1"
                     /translation="MAIELKKGQKINLTKKENSDLGEILVNLNWNQKVQKKGFFGSLR
                     SSNIDLDLGCLFEMKNGVKGAVQALGNAFGSLDNPPFAQLDGDDRTGSNTQGENLRIN
                     GNKIKDIKRILIYAFIYEGVANWSEADGIVTIKQKQDSDLVVKLDEHKNGYNMCSIAL
                     IENVNDETFSVEKVVKYFKGHREMDTEFHWGLKWVAGRK"
     gene            complement(3172..3969)
                     /locus_tag="ACGI59_003077"
     CDS             complement(3172..3969)
                     /locus_tag="ACGI59_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFG9740855.1"
                     /translation="MKFKKLLCLLLCLVLTLAVVGCSKAKDDKKIVVGATLVPGGELL
                     EELKPLIKEKGYTLEVKNFDDYILPNEALNNGEIDANLFQHEPYLKEAVKAKGYKIMA
                     GKKLYVCPAILYSYKIKSVDEFKKGDTIAISNNPSSCSKNLRYLESIGLLTLPKGDGL
                     VSPKDIIENPKGIQFKELDIAQIPSSLPDVTAAFIDTTYAVPAGLDAKKNGIYTAPIN
                     DEYANLLAFRTEDKDSEKIKVLQDVLTSDKARSLIEEKYKGIVIPTF"
     regulatory      complement(4082..4187)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(4279..5265)
                     /locus_tag="ACGI59_003078"
     CDS             complement(4279..5265)
                     /locus_tag="ACGI59_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HFG9740856.1"
                     /translation="MLLIYNEKTNPYFNLAMEEYLLKNFDEDLFILWRNEPSIIVGKN
                     QNTLSEINLEYIKEYSIPVVRRQSGGGAVFHDLGNINFTFIACNNNNFSDFRRFTQPI
                     IDLLKTLDVNAEFSGRNDLLIDGKKFSGNAQYNYKNKVMHHGTLLFSSEINDLSKALK
                     VKPIKFEGKGIKSVKSRVTNISEHLHSKMDILEFKDAIMDYLSKTNTDNTNYCLSEHD
                     IKQIEKLTKSKYETWDWNFGNSPKYTLSNELKYSGGNVEFNLNVDKGIITSIKFFGDF
                     FGKCDVAFVENKLIGIRHEENALRNTLDSIEINDYFLGADTDILVSGILGVK"
     regulatory      5429..5605
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            5749..7479
                     /locus_tag="ACGI59_003079"
     CDS             5749..7479
                     /locus_tag="ACGI59_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HFG9740857.1"
                     /translation="MRNKKINIIFLTTIMFIMSTVMVFAEEDIDTIALANAEKFGILT
                     LIPPLVAIILAFITKNVIISLLIGILSGSFIIKASGINVFATFIQAFLDLVDRALVSL
                     ADPWNAGIILQVLSIGGVINLVAKMGGAKAIAEALAKRAKSAKGTQLITWFLGLLVFF
                     DDYANSLIVGPMMRPVADKMKISREKLAFIIDATAAPVAGLAIISTWIGLEVGLIHDA
                     FESISIDVDAFGIFLNTIPFRFYNILILAFIVISALLLKEFGPMRKAEIKSRSRKISI
                     DLDEGVEELDDLAPKNGVKLSVWNAIIPIGTLIIVALASFYYSGYTSIMGGDDKALIQ
                     LFTNSPYSFEAIKEAFSASDASRALFQSALVASLVAIIMAVVKKIFTISEAIDVWIDG
                     MKSLVITGVILILAWSLSSVIKELGTAKFLIHLLSGSLPPFLLPSLIFGLGAIISFAT
                     GTAYGTMGILMPLAIPLAYSLNPDMSYVIVSTSAVLTGAIFGDHCSPISDTTILSSMG
                     AGCNHIDHVNTQMPYAIFTAVITIVFGYIPAGLGLPIYIVLPVAIAAIFVGIQIIGKK
                     VDEAEIELVE"
     gene            7589..7669
                     /locus_tag="ACGI59_003080"
     tRNA            7589..7669
                     /locus_tag="ACGI59_003080"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7623..7625,aa:Leu,seq:tag)
     gene            8004..8519
                     /locus_tag="ACGI59_003081"
     CDS             8004..8519
                     /locus_tag="ACGI59_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HXXEE domain-containing protein"
                     /protein_id="HFG9740858.1"
                     /translation="MEKYIWLFPLLFIFHDMEEIIGFGIWLKKNKSMLDKKYPFISKV
                     YENYSTEGMAFAVFEEFILCIIFCILTVITENQYVYLLWLGSFIAYTLHLVIHIGQSI
                     IIRKYIPSLITSIICLPISIWCISKSIYIVDCEMSTTILYSIIGIIIVALNLKFAQSL
                     IGKFTKWMSNI"
     regulatory      9011..9115
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            9325..11799
                     /gene="gcvPA"
                     /locus_tag="ACGI59_003082"
     CDS             9325..11799
                     /gene="gcvPA"
                     /locus_tag="ACGI59_003082"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HFG9740859.1"
                     /translation="MNELKRVSLYNIHKELGAKLVEFAGWEMPLEYEGINKEHEKVRK
                     SAGIFDVSHMGEVQIKGAESEKFIQNLVTNDISALKINDIIYTPMCYENGGVVDDLLI
                     YKFGEEDYLLVINAGNIDKDVAWIIKQSEGYNVDIKNISSEVSQLAIQGPKAEEILQK
                     ITDIDLNSIKFYKSIPSTKVCGCPCLVSRTGYTGEDGFEIYCKNKYVEIIWNEVLKVG
                     GEDICPAGLGCRDTLRFEAALPLYGHEINEHISPIEGGLSIFVKTNKESFIGKSILSK
                     EKESGAKRKLVGFEMQGKGMPRNGYDIRIGDKTVGFVTTGCASPTTGKILGMGIIDSE
                     YAKVGNEIGIAIRKKVVPAVIVKKPFYKKQYKKDNIILNKENKFSYIPATSEDKSKML
                     KVVGLNSVDELFSDIPEEVKLKRDLNLEIGKSELEVSKIVKRLSEENLSLEDLTCFLG
                     AGAYDHYIPSIIKHITSRSEFYTAYTPYQAEISQGTLQVVFEFQSMIAEITGMEIANA
                     SMYDGATAAIEACIMAMNQTRKSKIVVSKTIHPETLSVLRTYLQYKDCEIVEIDFCNE
                     YGTTDIEKLKASVDKDTACVLIQTPNFFGIIEEMEEIEKITHENKAMLIMSVDPISLG
                     VLKTPGEIGADIVVGEAQSLGNPLNFGGPYVGFLASKSKYTRKMPGRIVGQSLDVEGK
                     IAYVLTLQTREQHVRREKATSNICSNQALNALVASIYMATMGKEGFKEVGMQSMKKAH
                     YTYNKLVQTGKYKPIFKGKFFKEFAVQGNLNIETINDKLLEENILGGYNLEYNYPELK
                     NSTLLCVTEKRSKEEIDKLVGIMEGL"
     gene            11799..13256
                     /gene="gcvPB"
                     /locus_tag="ACGI59_003083"
     CDS             11799..13256
                     /gene="gcvPB"
                     /locus_tag="ACGI59_003083"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HFG9740860.1"
                     /translation="MKEYNSLLIDISKKGRKAYSLPKLDIDDIKIENMIDQDMARQSE
                     LNLPEVGELELVRHYTLLSNKNFGVDTGFYPLGSCTMKYNPKINEDMAALPNFTGMHP
                     YQSSDTAQGSLSLMYDLSRRLAEITGMDEVTLQPSAGSHGEFTGLMIIKAYHENRGDH
                     KRTKVIIPDSAHGTNPASAAMANFDVIQIASDKNGAVDINALKEVLNDEVAALMLTNP
                     STLGLFEKNIKEIATLVHEAGGLLYYDGANMNAIMGITRPGDMGFDVVHLNLHKTFST
                     PHGGGGPGAGPVGVKKELVPFLPIPVIERKEDKYVLNYDREKSIGKIKNFYGNFGVLV
                     RAYTYILTMGRDGLKEASEMAVLNANYIKESIKDDYILPIDTLCKHEFVLGGLPRGEA
                     NIKTIDIAKRLLDYGYHPPTMYFPLIINEALMIEPTESESIETLDSFIEAMKSVAKEA
                     KEEPELLKTAPHNTLVKRVDDARAVKKPILTWSQR"
BASE COUNT         5091 a         1594 c         2213 g         4626 t
ORIGIN      
        1 ttatactaga caaaaagaat aactcttaaa tataattaag ttatataata atatataatt
       61 ataatgatat aattttaata ggtggtttgt aattaaaaag tttataaatt atattaatag
      121 taataatcta taagaactta tatattagaa aaaaattttt gtatatataa aggactctaa
      181 agatgaacta tgtaaaacaa ggtttatata ttctaattgt atagaaaaat ttattgtaaa
      241 cgcatgtata ttattagaaa aaatagtttg gaggtattca ataaacataa aaaacaatat
      301 aggggataaa ggtgttttat gaagagattt ttgaggagaa ttatcttagt tttatttatt
      361 ttgttgattt ttataagttt tatttctatt aaattaattc ataatgttgg agattatgga
      421 aaattgatta attatgttgg gattgttcgt ggagctagcc aacgtctaac taaactagag
      481 atgaatcatc aaccaaatga tgaattaata gaatatattg atgaaatcct acaggagttg
      541 attacagggc atggagatta tggtttagta atcactgatt gtaatgaata caatgaggac
      601 ttacttttat tagaaaaaaa gtgggaagat ttaaatattg aaattaaaaa agtaagaatg
      661 aaagaacaga ataaccagtt attaagtata agtgaagagt ttttttctct tgcaaatgat
      721 acagtgttta aaatagaaaa cttttctaaa gaaaaaagta attacttaat gactttaata
      781 ataattatat ctattatagg tatattagca tgtatcatat tgattttaca atatagtaaa
      841 aaaatggtta gactggaaaa attaaatgta gatttaaaaa atattgcata taaagatgaa
      901 ctaacaggag taaatactat agaaaaattt aaattggatg caaatcaaaa tatttgtata
      961 cataaggata agaaatttgc tgtattctat attgattttg aaaactttaa atacattaat
     1021 gatatatttg gatatgatta tggtgacatg atactgaaaa gatatgctaa tcttatgatg
     1081 aatgacatag ggaaatatga aatatttgca agagaaattg cagatagatt tgttgcgtta
     1141 agatgctatg tagataagga agatttagtg gtaagacaac gagatgtaga caatgaacta
     1201 attaatataa caaatgaaat taaaaataaa catagtatta ctgttgttag tggaatttgt
     1261 tgtattgaag atgtaaatga aaaattaagt atagatggtt taataaatag agcaaatttt
     1321 gcacaaaaaa ctgttaagaa taaaccaggt actaattatg ctttttataa tgacagtatt
     1381 cgtaaaaaaa tgattgaaga aaatactatc aaaagtagaa tacatgaagc tattgaaaaa
     1441 agagaattta tagtttattt acagcctaaa gttaatttac atagtcaaaa aataaattgt
     1501 gcagaagctt tagttcgttg gcttactccg gataaaggta ttatatctcc agcaatattt
     1561 attccagtac ttgagaagaa tttttttatt gccttagtag ataagtatgt ttttgaggag
     1621 gtttgtaaat ggattagaaa gaggttagat gaaaataaac cttttgttca gatatcagtt
     1681 aatgtttcaa gaatacaatt ttataacact aaatttgtag aaacttattc caatatacaa
     1741 aataaataca ggatacctaa aaatactata gaaattgaat ttacagagag tgttgctttt
     1801 gagaatcaaa aacatctttt ggaaattata catgatttgc atgaaaatgg atttacttgt
     1861 tctttagatg attttggaaa aggttattcg tctttaagtg ttttaaaaga tttaccattt
     1921 gatgttttaa agcttgattc tatgtttttc aaggctagtt tagataaaga taaagagaaa
     1981 atagtaataa aaaatattgt acatatgtta aaagaactta atatcacaac tgttgctgag
     2041 ggcattgaat gcaaggaaca agttgaattt cttagggata ttgggtgtga tttagttcag
     2101 ggatttgtat tttataaacc aatgccaata ttagaatttg aaaaaatatt ggataaagag
     2161 tttgtttata atagctaaaa tgtactttat aaaatatgaa ctaattaagt aaaattattt
     2221 tgtactgaat aaatttttac tttatttaca gaataatgat tatttacaga aaagcgcgaa
     2281 aagtgtattc ctttaagtgt gggatggata gcacaaatag aggagaaaaa atattggcca
     2341 tataagaatg cataataata taaatattta aaaggtcagc attatataaa aattaaaaag
     2401 aaaataaata atttttctat aaaaaagaga ctatgagaaa atttactcat agtctcctaa
     2461 ataatgtatt tattttcttc ctgcaaccca ttttaatccc caatgaaatt cagtatccat
     2521 ttctctatga cctttgaagt atttaactac tttttcaaca ctgaatgtct catcatttac
     2581 attttctatc aatgctattg aacacatatt atagccattt ttatgttcat caagtttgac
     2641 aactaagtct gaatcttgtt tttgtttgat tgttacaatt ccatcagctt cagaccagtt
     2701 agctacacct tcgtatataa aagcataaat aagtattctt tttatatctt ttattttatt
     2761 accatttatt cttaaatttt ctccttgtgt attacttcct gttctgtcat ctccatctaa
     2821 ttgagcaaaa ggtggattgt ctaaactacc aaaggcattt cctaatgctt gaacagcacc
     2881 ttttacacca tttttcatct caaataggca acccaaatct aaatctatat tactgcttct
     2941 taaagagcca aaaaaaccct ttttttgtac tttttgattc caatttaaat taactaaaat
     3001 ttcacctaaa tcgctatttt ccttttttgt aaggtttatt ttttgtccct tttttaattc
     3061 tatagccata gtaatcttcc tccacaaaca tagatttatt taaatggtag cacttaataa
     3121 gtcaatattc aacaagatta gaataataat attaaaatta tactaaatat actaaaaagt
     3181 aggaatcaca atacctttgt atttttcttc aattaagctt cttgctttat ctgatgtaag
     3241 aacatcttgt agaactttaa tcttttcact gtctttatct tcagttctaa atgctaaaag
     3301 atttgcatac tcgtcattta taggagctgt ataaattccg tttttcttgg catcaagacc
     3361 agcaggtaca gcataagtag tatctataaa tgcagcagtc acatctggaa gagaagatgg
     3421 aatttgagca atatctaact ctttgaattg tattcccttt ggattttcta ttatgtcctt
     3481 aggactaact aatccatccc ctttaggtaa cgttaaaagt ccaatacttt caaggtatct
     3541 aaggttctta gaacaagaag atgggttatt gcttattgca attgtatctc cttttttaaa
     3601 ttcatcaaca gattttatct tataagagta aagtattgct gggcatacat atagtttttt
     3661 accagccatt attttgtaac ctttggcctt aacagcttct tttagataag gttcatgttg
     3721 aaatagattt gcatcaattt caccattatt taaagcttcg tttggaagta tgtaatcatc
     3781 aaagttttta acttctaaag tatatccttt ttctttaata agtggtttta attcttctaa
     3841 taactctcct cctggaacta aagtagcacc aacaacaatt tttttatcat ctttagcttt
     3901 agaacatcca accacagcta aagttagtac tagacataat aacaaacata ataatttttt
     3961 gaatttcata taaattccct cctgaatata attgattagt taaaaataaa ataccattct
     4021 ttatcacact gcaaaataaa aataagcctg agtttaaact caggctttaa aagctaattt
     4081 agactcatct ttcagtataa tactgcagga tttagcacca atgtatataa aaatatactg
     4141 gttgctgggt ttcatagggc cagtcccttc accactctgg ataagaagta tcaattttta
     4201 attatgttat aaatattatc atgatttttt acgtctgtaa acataatttt ataaatattt
     4261 taaaatcttt aatgtatttt atttaacacc taaaattcca gaaactagaa tatcagtatc
     4321 agcacctaag aaatagtcat ttatttcaat agaatccaga gtatttctca aagcattttc
     4381 ttcatgcctt ataccaataa gtttattttc tacaaatgca acatcacatt ttccaaaaaa
     4441 atctccaaaa aattttatac tagttattat gcctttatca acatttaaat taaactcaac
     4501 atttccacca gaatatttta attcatttga aagagtatat tttggtgaat taccaaaatt
     4561 ccaatcccaa gtttcgtatt ttgatttagt aagcttttct atttgtttta tatcatgctc
     4621 actcaaacaa taattagtat tatcagtatt tgttttagaa agataatcca ttatagcatc
     4681 tttaaattct aatatgtcca tttttgaatg taaatgttca cttatattgg ttacacgaga
     4741 ttttacagat tttattcctt ttccttcaaa ttttataggt ttaaccttta atgcttttga
     4801 gaggtcattt atttcagatg aaaataaaag agtcccatga tgcattactt tgtttttgta
     4861 gttgtattga gcatttccag aaaatttctt gccatctata agaaggtcat ttcttccaga
     4921 aaattcggca ttgacatcta gtgttttaag taaatctatt atgggctgag tgaatcttct
     4981 gaaatcacta aaattattat tgttacaagc tataaatgta aaatttatat ttcccaaatc
     5041 atgaaataca gctccaccac cagactgtct cctaacgaca ggtatagaat attcttttat
     5101 atattctaaa tttatttcag aaagtgtgtt ttggtttttt ccaacaatta tagaaggttc
     5161 attcctccat aaaataaata agtcttcatc aaaatttttt agaagatatt cttccattgc
     5221 taaattaaaa taagggtttg ttttttcatt ataaattaat aacattaaag tagttcctcc
     5281 atagaattga taaaattttt attatgaata caattaattt tataattcga caataatata
     5341 aaaactcctt taaattagaa attttaaaaa ctaccttgca aagtttaaat aaataatata
     5401 tactcaaaat atgtttaata aaagtgaata gaagggtaga ggcgcaataa tctatcagta
     5461 ctaataagga gttaagcaca gagagttatt aggaaaggag atattgccga agttttataa
     5521 tcgatgcttt aagattatat tgctgggggt acatagaaaa tatgtacaac tgtcacaggt
     5581 ttatttgtgg tgagctatca tttaaggaat tatattatat ttatttttac tattgttgat
     5641 atattgtaat gtttaagcct tgagtgttta ccactcaagg cttttttagt tttattgtaa
     5701 cataataccc tgtacagttc acaaaattaa aaggagaggg agatttatat gaggaacaaa
     5761 aaaattaata ttatttttct gacgacaatc atgttcataa tgtcaacagt aatggttttt
     5821 gcagaagaag atatagacac tattgcacta gccaatgcag aaaaatttgg gattttaact
     5881 ttaataccac cattagttgc tataatactc gcatttataa ctaaaaacgt aataatttct
     5941 ttacttatag gtattctttc tggtagtttt ataattaaag ctagtggtat taatgttttt
     6001 gcgacattta tacaggcatt tttagattta gtagatagag ctttagtatc tttagcagac
     6061 ccttggaatg cggggataat actacaggtt ttgtcaattg gaggagtaat aaatcttgtt
     6121 gctaaaatgg gaggagctaa agctatagct gaagcacttg ctaaaagagc aaagtcagca
     6181 aaagggacac agcttataac atggtttttg gggcttttag tattctttga tgattatgcc
     6241 aattcactaa tagtaggtcc tatgatgaga cctgttgcag ataaaatgaa aatatcgaga
     6301 gaaaagctag catttataat agatgccaca gcagcaccag ttgctggttt agctataata
     6361 tctacatgga taggtcttga ggttggttta atacatgatg cttttgaaag tataagtata
     6421 gatgtagatg catttggtat atttttaaat acaataccat ttagatttta caatatatta
     6481 atattagctt tcattgtaat atcagcatta ttattaaaag aatttggtcc aatgagaaaa
     6541 gctgagataa aatctagaag tagaaaaatt agtatagacc ttgatgaagg cgtagaagag
     6601 ttggatgatt tagctccaaa aaatggagta aaattgagtg tatggaatgc tataatccca
     6661 ataggaacat tgataatagt tgcattagca tcattttatt atagtggata tacatctata
     6721 atgggaggag acgataaggc tctgatacaa ctatttacaa attctccata ttcttttgaa
     6781 gcaataaaag aagcatttag tgcatctgat gcatctagag cattattcca gtctgcactt
     6841 gttgcaagtt tagttgctat tataatggct gtagtaaaaa agatttttac aatatcagaa
     6901 gcaattgatg tttggataga tggaatgaaa agtttagtaa tcactggtgt aatattaata
     6961 cttgcatggt cattaagttc agtaataaaa gagttaggta cagctaaatt tttaatacac
     7021 ctactatcag gttcactacc accattctta ttaccaagtt taatatttgg tttaggagca
     7081 ataatatctt ttgctactgg aacagcttat ggaacaatgg gtatacttat gccacttgca
     7141 attccattag catactcatt aaatcctgat atgtcttatg taatagtaag tactagtgca
     7201 gttttaacag gtgcaatatt tggagaccat tgttctccaa tttcagatac aacaatactt
     7261 tcttcaatgg gagctggctg taatcatata gaccatgtta acactcaaat gccatatgct
     7321 atatttacag cagtaatcac tattgtattt gggtatatac cagctggact tggattgcca
     7381 atatatatag tattaccagt ggcaatagct gctatatttg taggtattca aataattggt
     7441 aagaaagttg atgaagcgga aatagaactt gtagaataaa gatataaata agtttcacct
     7501 tttattatca ctattttgtt agtttttaga taattttaaa aagttattga aaataatggc
     7561 gaaatatagt aaaataaaag agtgataagc ggatgtggcg gaattggcag acgcactaga
     7621 cttaggatct agcgccattg gcgtgggggt tcgactccct tcatccgcac tttacagtac
     7681 ttaattgtac tgaaaaaaac tggggttgct ggctttcgga gtgggctatc ccgtttttat
     7741 ttttttgtat atataataga ttggcacttt agtataaggt gcttttttta taggcaaaaa
     7801 cagtacattt atttctgttt tattatcatt catctattag aatatacttt agttaagaat
     7861 gtgttaataa gcagtgttca cattcttgat aaaatgtgga ataatatgta tttttactaa
     7921 gacggaaatg aaataattaa accattaaat aatattataa gatgatgaat aagtagataa
     7981 atcaaatttt gtaggaggat attatggaaa aatatatatg gttgtttcca ttacttttta
     8041 tttttcatga tatggaagag ataataggtt ttggaatatg gcttaaaaaa aataaatcaa
     8101 tgcttgataa gaagtatcca tttatcagta aagtttatga aaattatagt actgaaggca
     8161 tggcatttgc tgtgtttgaa gaatttattt tatgcattat tttctgtata cttacagtta
     8221 taacagagaa tcagtatgta tatctactat ggttaggtag ttttatagca tataccttac
     8281 atttggtaat tcatataggg cagtcaatca taataagaaa atatatacct tcacttatta
     8341 ccagtatcat atgcttacca ataagcattt ggtgtataag taagagtatt tatatagttg
     8401 attgtgaaat gagtacaaca attctgtaca gtataattgg cattataatt gtagcattga
     8461 atttaaagtt tgcacagtcg ctgattggaa aatttacaaa gtggatgtca aacatataaa
     8521 ttttagttta ttggacagtt caaggaatca gaaattggaa ttttatagta ttaatatttt
     8581 atggtattaa tataatgtat aaaattttat agcaatgtat ttcataagta taaagtgact
     8641 agaattttaa gaaatataca aattctataa aaactaaata atttcaagta tattttaaaa
     8701 atcttatttt tcacaagttt aaaattgtaa acgatttcat caagtctatg aatatacttt
     8761 taacatgtta tagtaaatct caatataaat ttacatttgg ttctttgtgt gttattttaa
     8821 taataaatta agtgaaaatg ttattttaca ttaatatata atatctttgc ataagagtga
     8881 ataatataaa agagaaataa ttgataataa aaacttaaca aaaaaattaa tacataaaaa
     8941 aaaataaata taatgtgttg acatttaatt gaaatattca tacaataaaa ttgaaaataa
     9001 atatttccaa atgagattag cgagagagtc ctttatacga aaaggcgccg aagaagaaat
     9061 ttaagaaatg ccaattttta gagaagcttt caggcaaaaa tatcgctaat tgatgggact
     9121 ctggagagac ttaagttaag atactataag tcaccgaaga agcaaaataa tattaaatta
     9181 ttaaatcttt caggtataca aacagggata tataggctag gattattttc catgctgata
     9241 tatctttttt ttatgaaaaa gtacaggaaa taatatcaaa gttacttaaa tacagtaggt
     9301 ctaattttct agggagggtt atatatgaat gaattgaaaa gagtatcttt gtacaatata
     9361 cacaaagagc ttggagcaaa acttgttgaa tttgctggtt gggaaatgcc tttagaatat
     9421 gagggtataa ataaagagca tgagaaggtc agaaaaagtg caggtatatt tgatgtatct
     9481 catatgggag aggtacagat aaagggagca gagtcagaaa aatttataca aaacctagtt
     9541 acaaatgata taagcgcatt aaagataaat gatattatat atacacctat gtgttatgaa
     9601 aatggtggag tagttgatga tttattaata tataaatttg gtgaagaaga ttatttatta
     9661 gtaataaatg caggtaatat agataaagat gtagcatgga taataaaaca aagtgaagga
     9721 tacaatgtag atataaaaaa tatatctagt gaagtgagtc aattagcaat tcaaggacct
     9781 aaagcagaag aaattcttca aaaaatcaca gatatagact taaactcaat aaaattttat
     9841 aagtcaattc catcaacaaa agtatgtggt tgtccttgtt tagtttcaag aacaggatat
     9901 actggggaag atgggtttga gatatactgt aaaaataaat atgtagaaat aatttggaat
     9961 gaagttctaa aggttggagg agaagatata tgtccagctg gtctaggttg tagggatact
    10021 ttgagatttg aagcagctct acctctatat ggacatgaaa taaatgaaca catatctcca
    10081 attgaaggtg gtttatctat ctttgtgaaa acaaataaag aatcatttat tggcaaatca
    10141 attttatcaa aagaaaaaga aagtggagct aaaagaaagt tagtaggatt tgaaatgcaa
    10201 ggtaaaggaa tgcctagaaa tggatatgat ataaggattg gtgataaaac agttggattt
    10261 gtaacaacag gatgtgcatc tccaacgaca ggaaaaattt taggtatggg aattatagat
    10321 tctgaatatg caaaagtagg aaatgaaata ggtatagcaa tcagaaaaaa agttgttcct
    10381 gctgtgatag ttaagaaacc tttttataaa aaacaatata aaaaagataa tattatatta
    10441 aacaaagaaa ataaattttc atatatacct gctacaagtg aagataaatc aaaaatgtta
    10501 aaagtagttg gattaaattc agttgatgaa ttattttcag atataccaga agaagtaaaa
    10561 ttaaaaagag atttaaattt ggaaattgga aaatctgaat tagaggtaag caaaatagta
    10621 aaaagattgt cagaagaaaa tttaagttta gaagacttga cttgttttct tggtgctggt
    10681 gcatatgacc attatatacc atcaattata aaacatataa cttcaagatc tgaattttat
    10741 acagcatata ccccatatca agctgaaata agtcagggaa ctttacaagt agtttttgaa
    10801 tttcaatcta tgattgcaga aattacaggt atggaaattg caaatgcatc aatgtatgat
    10861 ggagcaactg ctgcaataga agcatgtata atggcaatga atcagactag aaaaagtaaa
    10921 atagtagtgt caaaaactat tcatcctgaa actctgtcag tattaagaac ttacttacaa
    10981 tataaagatt gtgagatagt agaaattgac ttttgtaatg aatatgggac tacagatata
    11041 gaaaaattaa aagcttctgt agacaaagat acagcatgtg ttcttataca aactcctaat
    11101 ttctttggaa ttattgaaga aatggaagaa atagaaaaga taactcatga aaataaggct
    11161 atgttaatta tgagtgtaga cccaatatca ttaggagttt taaaaacacc aggtgagata
    11221 ggtgcagata ttgttgttgg agaagctcaa tcacttggaa accctcttaa ttttggaggt
    11281 ccttatgtag gatttttagc tagcaaatct aaatatacta gaaaaatgcc aggaagaata
    11341 gttgggcaaa gtttagatgt agaaggtaaa attgcatatg tattaacttt acaaactaga
    11401 gaacagcatg taagaagaga aaaagcaact tctaatatat gttcaaatca agctttaaat
    11461 gctcttgtag cttcaatata tatggctact atgggtaaag aaggatttaa agaggttggt
    11521 atgcagtcta tgaaaaaagc acattatacc tacaataaac ttgtacaaac tggaaaatat
    11581 aagccaatat ttaaaggtaa gtttttcaaa gaatttgctg tacaaggaaa tcttaatata
    11641 gaaactataa atgataaact cttagaagaa aatatattag gaggttataa tttagaatac
    11701 aattatccag aattaaaaaa ttcaactttg ctttgtgtga ctgagaaaag aagcaaagag
    11761 gaaatagata agttagttgg aataatggag ggattataat gaaagaatac aatagtttgt
    11821 tgatagatat atctaaaaaa ggtaggaaag catactcact tccaaagtta gatatagatg
    11881 atataaaaat agaaaatatg attgaccaag atatggcaag acaaagtgag ttgaatttgc
    11941 cagaagttgg tgaattagaa ttagttagac attatacatt attatcaaat aaaaactttg
    12001 gagttgatac aggtttttat cctttgggtt cttgtactat gaaatataat cctaaaataa
    12061 atgaagatat ggcagcactt ccaaacttta ctggaatgca tccataccaa tcttcggata
    12121 cagctcaagg ttctctttct ttgatgtatg atttatcaag aagacttgca gaaattacag
    12181 gtatggatga agttacacta caaccttcag caggttctca tggtgagttt acagggctta
    12241 tgattataaa agcatatcat gaaaatagag gtgaccataa gagaaccaaa gtgataattc
    12301 cagattctgc acatggaaca aatccagcaa gtgcagctat ggcaaacttt gatgtgattc
    12361 aaatagcatc tgataaaaat ggagctgtag acataaatgc attaaaagag gttttgaatg
    12421 atgaagtagc agctcttatg cttactaatc caagtactct tggtttattt gaaaaaaata
    12481 taaaagaaat agctactctt gtacatgaag caggaggact tttgtactat gatggtgcaa
    12541 acatgaacgc tattatgggg ataacaagac ctggagatat ggggtttgat gtggttcatc
    12601 ttaatcttca taagactttt tcaactcctc atggaggtgg aggtccagga gctggtcctg
    12661 ttggagttaa aaaagaatta gtaccttttt taccaatacc agtaattgaa agaaaagaag
    12721 ataagtatgt actaaattat gatagagaaa aatctattgg gaaaattaaa aatttctatg
    12781 gtaattttgg agttcttgta agagcttaca catatatact aactatggga agggatggac
    12841 ttaaagaggc tagtgaaatg gcagttttaa atgctaatta tataaaggaa agtataaaag
    12901 atgattatat tttgccaata gataccttgt gtaagcatga gtttgtatta ggtggattgc
    12961 caagaggaga ggccaatata aaaactatcg atattgcaaa aagactacta gattatggat
    13021 accatcctcc aacaatgtac tttcctctta ttataaatga ggcacttatg atagaaccta
    13081 cagaaagtga gagtatagag acattagata gttttataga agctatgaaa agtgttgcta
    13141 aagaggcaaa ggaagaacca gaactattga aaactgctcc acataatact ttggtcaaaa
    13201 gagtagatga tgcaagggca gtaaagaaac ctatactaac atggtcacaa agataaatag
    13261 ttaaaaagta gagatttaaa gtataaagag aattaaattt gtaagaaaaa gctatctaac
    13321 ttaatctagt ttagatagct tttttgtata ttagtatgta tggtattttc tatataaagt
    13381 tttaaattat attagtgctt atatagaata ttgatatgat gattatgtat agatattaat
    13441 aagaattgtt gaatttatct tgccttaact ttatttactc ttttatatta agaaaaactt
    13501 aactaaaata tagcgaaatt cata
//
LOCUS       DAYAPA010000120        13518 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.122, whole genome shotgun sequence.
ACCESSION   DAYAPA010000120 DAYAPA010000000
VERSION     DAYAPA010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13518
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.122"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            801..1463
                     /locus_tag="ACGI59_003084"
     CDS             801..1463
                     /locus_tag="ACGI59_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9740861.1"
                     /translation="MSILEIKNLNKIYGKSETEVKALSQINLKINSGEFVVIVGKSGS
                     GKSTLLHIMAGLETPTNGDVIIDNINISSLDEKKRSALRKEKLGLIFQSYNLIPVLTV
                     EENIKLPTINIKNKDEAYINELMELLGIKDRRMYLPNQLSGGQQQRVAIARALVNKPS
                     IVFADEPTGNLDTKTESDVLSLLKESQKKYNQTIIMITHNIDITKYADRVIEIEDGFI
                     RE"
     gene            1472..3979
                     /locus_tag="ACGI59_003085"
     CDS             1472..3979
                     /locus_tag="ACGI59_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFG9740862.1"
                     /translation="MKLNYIVKKYIMGNKKNTLLIIVSIVISTALFLIMNIISEDATN
                     LMINQAKNEFTLKHAQYINPKDEEIKYIENNTSIDKVGKSMLLGISDIGKGQTLQILS
                     QDKVAEELNDIYTLEKGKLPVAENEIAIDSWYIKQKKIKNPIGKRITLDYRRQGIDQE
                     DLYTGEKEFKITGILNSNPILKAQGTSIGLISNDCAIKNIPTKNKYDQVMFTFKKEKN
                     IQRQSQKLIKNGNLNENNIYFNNELLIAISDSMNLKIPYIIVNIVLALATILLIYNIF
                     YILVSNRIKDFGIFRALGFNPNDIFKIMILEVSIYSIISISIGLILGGIIASLSREYV
                     IGVIYNVNYINSIKNQNYINTYIISILLSLGTIIISVSKPLMLSVKTDPMICIRRNNE
                     KVDIKQNSFINKFMIKLFKDYGNIASKNIQRNKKRTNLSIASMVIIFFLMATIYTKST
                     SNFLSDGVLKYWIQGDYLLHNIDISTIRSNNKGYDKNVLQEIKAIDGVTKVNSYRHKW
                     FNIKIDDKSINKNSDYWKKNKDNIEARSEVKDGIKVYNNSFEFLGIEDTDILEDFLIN
                     GKENIDKFSKQPYLYITKKSSDSLNIKKGDRIKVYFDIIDSKTNNFTKTISQEFIVCG
                     IITTLPVTSQVGAEFGGVISTNQFNKFTGISSYERFDIWTSKLANDKYVESELNKITE
                     KTNKGILIPYKAETAEYEKIDNQKTLIMVLVTGIIVILSLFNCCNTIVTSINSRSREF
                     ALFRGIGISKHEINKIVKLESYIYIIVSFCISIIPILIVRSIIIKPFESINLINWKFI
                     GAIILIVFILVSIIMITTLKTLNKIQNENFMEEIKTL"
     gene            4310..4654
                     /locus_tag="ACGI59_003086"
     CDS             4310..4654
                     /locus_tag="ACGI59_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9740863.1"
                     /translation="MYCLSVVLAIYGIYVFINCTEYIYTMLSTKHLTVKGNEYTIINY
                     YMSNFSQYIIFAILLASMGFVIQKIVHMHFSEIELSGNYSTTVRNISDISLDNDNEMF
                     NRGNIENSILKK"
     gene            4892..5578
                     /locus_tag="ACGI59_003087"
     CDS             4892..5578
                     /locus_tag="ACGI59_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFG9740864.1"
                     /translation="MSSILLVEDDLSLIYGLEYSIQKNGFSVDVARTVEEALQIYKEK
                     NYDLLLLDVSLPDGDGFDICKRVREASNVPIIFLTASDEEVNVVMGLDMGGDDYITKP
                     FKLNELISRIKALLRRYNFTSENVTELKSNNITVKLLENRVFKNEIELELTTAEYKLL
                     CLLMKNKNIVLTRKNILDKLWDGNGSFVDDNTLSVYVRRLRNKIEDNPENPKNLLTVR
                     RMGYKWNVIK"
     gene            5589..6830
                     /locus_tag="ACGI59_003088"
     CDS             5589..6830
                     /locus_tag="ACGI59_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFG9740865.1"
                     /translation="MKVFSNKDIKIFFLVIISVLLMFIVVGQIVVINITNDYKSALLE
                     HDYNIAGYLNSMGVDKSKIPAVFTTNEDKYTLKGKEILNTARYDFGTDDSFMPSVKLF
                     QGKYIKTMFLYLFVFFTMIIFILYVYFFRQQNKIEEASYKIDSFMNGNINVRLNTYEE
                     GSLSKLFGSIDAMSTSLNTHILKEKQNKEFLKNTISDISHQLKTPLTALMMYNQILQE
                     ESHNSEIVENFVQKSQNELERIESLIQNLLKITKIDSKTIILNKASVNVKKLITKILY
                     SFETRAEKEGKSISIRGLENTNLFCDYEWISEALSNLIKNALDNTKENDKIMIEWVET
                     PITTIISVSDTGNGIHIEDIHHIFKRFYRSKFSKSNQGLGLGLPLVKSIIEQHNGTIT
                     VESNFLQGSTFTLSFLKLTNM"
     gene            6901..7584
                     /locus_tag="ACGI59_003089"
     CDS             6901..7584
                     /locus_tag="ACGI59_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002584132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9740866.1"
                     /translation="MDILVVNNLCKTYGNGYTKVEALKDVSFTVSKGEFVTVVGESGS
                     GKSTLLNLLGGLDTPTSGKIFVDGKEICSMKEEKQTIFRRRNIGFIFQSYNLIPELTV
                     EQNIIFPLLLDYKKPDTIYLDEVLTILGLKDRKSHLPNQLSGGQQQRVAIGRALITKP
                     SIILADEPTGNLDSKNSSDVISLLKVTAEKYNQTIIMITHNTNIASTADRILQVSDGM
                     LKDLGVNRE"
     gene            7581..9914
                     /locus_tag="ACGI59_003090"
     CDS             7581..9914
                     /locus_tag="ACGI59_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9740867.1"
                     /translation="MRSYLELVPQYAKVHKKKNRTTILCIVIAVCLVTAIFGLADMAI
                     QCQKVQFIKDDGNWHIVLKNLNEEAISEISSRVDIAHYGRGQVIDNGEFKSKEILIQG
                     LDEDLAKQLGLEIIKGRYPVVENETLIDVDAVKSFNLKVGDFIKIKLADGRNKNYKIV
                     GEYSSLSRLRASDKHGLMLSILGTQAIKKSETYDYYYITFKDKVNIRKATSDIKNTFN
                     LSDKQIGKNEKLLSIIGQGDSDYSKPLYLTAGGLFILVLIASIIMIYNSFNMGVIERI
                     KFFGLLRCLGASKLQVKKFVVLESLILSVKAIPLGLLLGCIVTIISSIFLKYVNTELF
                     GSMPILKVSFIGIVFGIIVGFLTVILSAIVPAKKASGVSPLSAIRGNLMFKEILTSGK
                     ASKIANAGSSKIDVSMGINHAFSRKKSFILMTSSFAISIVLFLGFSVFIDFMYQALKP
                     IKPEAAHISIVDADFKSVLTKDDINEISNIKGIKKVYSRMMTDVEATYNNKEATSQLI
                     SYEKNQFDWAKKYLVKGEINEDKLESENIGLVEEGHGFNIGDKIYIKNGNSKKEIEVA
                     GILSSISFDVKDKYIGNIITSENTFTSITDIKDYIIIDTQVDEDAPEDLIQNIRNVLD
                     DNLEVRDKRQGNLEAKNSFYTMAVFVYGFVFIIAIISMFNIINSMNISVTSRINYYGI
                     MRAIGMSNKQLRKMVIVESSTYAVSGCLLGSVLGLILHRYIFVSLVTLKFHIDWQIPF
                     DLLFIIVVTMIIITLLAVRKPIKKICELDIIEVVNAQ"
     gene            complement(10022..10584)
                     /locus_tag="ACGI59_003091"
                     /pseudo
     CDS             complement(10022..10584)
                     /locus_tag="ACGI59_003091"
                     /inference="COORDINATES: protein motif:HMM:NF037635.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(10698..12047)
                     /locus_tag="ACGI59_003092"
     CDS             complement(10698..12047)
                     /locus_tag="ACGI59_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFG9740868.1"
                     /translation="MEKAKKLGLFSMILLGINSIIGSGIFLLPGKVYNLAGQNSMFIY
                     IFATLLVLSILLCFAEVGSMFDKNGGAYLYSKKAFGDFIGFEVGTMSWVIRIISWSTL
                     AVGFATALGSFWPESATEYKGYIAAILVTLLSINSLFGIKSTKIMNNVITIAKLVPLI
                     VFIIVGIFFIKFVNIVPSGNVVNSSMGPAIILVFYAFTGFESFIVASGEMENPKKNLP
                     VALITTIFICAIIYILIQIVCMGILGDRLFENSIPIADASSVFLGNYGKFFISVATLI
                     SIFGINIGSSIVTPKCGSSLAEEGSLPAFIGKTNKYGAPYVAIIISLICCIPLVLTGS
                     FEQLAVMSVIARFAQYIPTCLSVIVLRKRTDVKASFKIPFGPVIPMVAILGSLWLLQQ
                     AWAEDISKPITQNRVLIGLGAMLLIAPLYIFMKKNKEIEHKNKSEDSKLYISREKIQ"
     gene            complement(12084..13304)
                     /locus_tag="ACGI59_003093"
     CDS             complement(12084..13304)
                     /locus_tag="ACGI59_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFG9740869.1"
                     /translation="MQQALKLKYQTKELGKDFVIESCNSIKPWLINIRRELHKIPELA
                     LEENLTKQKVISYLKEIGIDYMEFTKHNGIMAYILKESADKTICIRADMDALPIEEEN
                     NIPYKSIHSGKMHACGHDAHTTMLLGACKVLHSIKDKLNVNVKFLFQPAEEGFGGAKF
                     LVEDGCLENPKVDYIFGLHVMPHIETGFIEIKYDTLNASVDTIKISIKGKRAHGAYPE
                     NGIDAIVTASQIVTSLQTIISRNLEPNNAAVLTIGKIYGGDAHNVICEDVKLEGTLRT
                     LNSKTRNFMIDKIAKIVGDTASAFGCVGTLHVSDENYPAVINEKELVDTVISNTKELL
                     GEEKFILRPNPSLGGEDFSFYTEHCKGAFFHLGCKNEEKGLISPLHTSSFNIDEDCLP
                     IGVMMHVMNTLYFN"
BASE COUNT         5348 a         1530 c         2041 g         4599 t
ORIGIN      
        1 atgtttaaat cagaaatata aataaatatt attataatcg ttattaaaga ccaaaaatcc
       61 caccttagaa gtttgtatca tatgaaacct ttatagatta tgaaagcaat aatatagtgt
      121 atgatatata aaataagtta taaaagtatt tttttagata ttcaaaaaga cattgtaagt
      181 tatattaata atatgggtat caacgaattt tggatataat gagtttttca acttaaagtt
      241 atgtaataat gaatgtaaag aatatttaat gaaatcattt tgttggttat gtctagctga
      301 atacaataat cttttagata aagattttat atttgaagat aggtgtgaaa aaattaagaa
      361 gatattaaca tcagcagttt agaggcaaag catgtaaagt aaagtctggg taactaaaag
      421 tagtaattta gattgtaact attgttataa atatgaaaat gaggaaacag gaaatataag
      481 ttaggatgta gaaaataggt tactaaataa aaagcttaat tatataatca aagaaatagg
      541 tatatactgt acaatttcat ggtgaagaac ttttcctaaa taaaaaggtt ggtaaaagag
      601 atataagaaa taaaattcaa ttataagata tggtatgata actaaaggca ctatatggag
      661 tgaataggcg gaagaatttt ttgaaaatta taaaaatagt ttctgttgct acatatcaat
      721 aagtatagat agagattgca aaatccatag caaaaatcga atttataagt gtaaataaca
      781 tgtaagacaa ggagtaaatt atgagtattt tagagataaa aaacctaaat aaaatatatg
      841 gtaaatcgga aacagaggtg aaagcgttaa gtcaaataaa tctaaaaata aatagtggag
      901 aatttgttgt gatagtaggt aaaagtggaa gtgggaaaag tactttacta cacataatgg
      961 caggacttga aactccaaca aatggtgatg tgattattga taatataaat atatcatctc
     1021 ttgatgaaaa gaaaagatct gcattacgta aagaaaagtt gggattaata tttcaatcat
     1081 ataatttgat tccagtttta actgttgaag aaaatataaa actaccaact ataaatataa
     1141 aaaataaaga tgaagcttat attaatgaat tgatggagtt actaggaata aaagatagaa
     1201 gaatgtattt gccaaatcaa ttatcaggtg ggcaacaaca aagagtagca atagcaaggg
     1261 ctcttgtaaa taaaccatca attgtatttg ctgatgaacc tactggaaat ttagacacaa
     1321 agacagagtc agatgtatta agtttattaa aagaatctca aaaaaaatat aatcagacaa
     1381 taataatgat aacacataat atagatataa caaaatatgc tgatagagtt atagaaatag
     1441 aagatggatt tataagagag taggtagatt tatgaaactt aattatattg taaaaaaata
     1501 tataatggga aataagaaaa atacattatt aataattgta agtattgtaa tttcaacagc
     1561 gttgttttta attatgaata taataagtga agatgcgaca aacttaatga taaatcaagc
     1621 taaaaatgaa tttacattaa aacatgcaca atatatcaat ccaaaagacg aagaaataaa
     1681 atatatagaa aataacacaa gtatagataa agttggaaaa tcaatgcttt taggaattag
     1741 tgatattggt aaaggtcaaa cactacaaat tctatctcag gataaagttg ctgaagaact
     1801 aaatgatatt tacacactag aaaaaggtaa attaccagta gctgaaaatg aaatcgctat
     1861 agattcatgg tatattaaac aaaaaaagat aaaaaaccct ataggaaaaa ggataacatt
     1921 agattataga agacaaggga tagatcaaga agatttatat actggggaaa aagaatttaa
     1981 aataactggt atcttaaata gtaatcctat tttaaaagct caaggaacat caattggcct
     2041 tataagtaac gattgtgcta tcaagaatat tcctactaaa aataaatatg atcaagttat
     2101 gtttactttt aaaaaggaaa aaaatataca gagacaatca caaaaactta ttaaaaatgg
     2161 aaatttgaat gagaataaca tttattttaa taatgaatta ttaatagcta tatctgatag
     2221 tatgaattta aaaatacctt atataatagt taatatagta ctagcactag caactatatt
     2281 attaatttat aatatttttt atatattagt atcaaataga ataaaggatt ttgggatatt
     2341 tagggcttta gggtttaatc caaatgatat ttttaaaata atgattttag aagtatctat
     2401 atactctatt ataagtattt ctataggtct aattttaggt gggataatag ctagtttatc
     2461 aagggagtat gttataggag taatatacaa tgtcaactat ataaattcta taaaaaacca
     2521 gaactatata aacacataca taatttctat attattaagt ttaggaacta taataatatc
     2581 agtttcaaaa cctcttatgt tatcagttaa aactgatcca atgatttgta taagaagaaa
     2641 taatgaaaaa gtggatataa aacaaaattc ctttataaat aaatttatga taaaactttt
     2701 caaagactat ggtaacatag catcaaaaaa tatacaaaga aataagaaac gtacaaattt
     2761 atctatagca tcaatggtaa ttatattttt cttaatggca acaatataca caaagtctac
     2821 tagtaacttt ttaagtgatg gtgtattaaa atattggata caaggagatt atttattaca
     2881 taatattgat ataagtacta tacgttcaaa taataaaggt tatgataaaa atgtactaca
     2941 agagataaag gcaattgatg gtgtaacaaa agtaaactca tataggcata agtggtttaa
     3001 tataaaaatt gatgataaaa gtataaataa gaactctgat tattggaaaa aaaataaaga
     3061 taatattgaa gctagatcag aagtaaaaga tggaattaag gtatataata attcttttga
     3121 atttttagga atagaagata cagatatact agaggatttc ttgataaatg gaaaggaaaa
     3181 tatagataaa tttagtaaac aaccatatct gtatataaca aagaaatcca gtgattcgct
     3241 taatataaaa aaaggagata gaataaaagt atactttgat attatagatt caaaaacaaa
     3301 taattttacc aaaacaatat ctcaagaatt tatagtatgt ggaattataa ctacattacc
     3361 agtaacctca caagtgggag ctgaatttgg cggagtaata tcaacaaatc aatttaataa
     3421 gtttacagga atatcatctt atgaaagatt tgacatatgg acgagtaaac tagccaatga
     3481 taaatatgtt gaaagtgagt taaacaaaat aacagaaaaa acaaataaag gaattttaat
     3541 tccatacaaa gctgaaacag ctgaatatga aaaaattgat aatcaaaaaa cactaattat
     3601 ggtattagta actggaatta tagttatatt atctttattt aattgttgca atactatagt
     3661 tactagtatt aatagcagaa gtagggaatt tgcactattt agaggaatag gaatatcaaa
     3721 acatgaaata aataaaattg ttaaattgga aagttatata tatattatag taagcttttg
     3781 tatatctata atacctattt taattgtaag aagtataata ataaaacctt ttgaaagtat
     3841 caatttaata aattggaaat ttataggggc tattatacta atagtattta ttttagtatc
     3901 tataatcatg ataactacac taaaaacatt gaataaaatt caaaatgaaa attttatgga
     3961 agaaataaag actttatagt gaacagccta taaattaaag aggttttata tgaatttaat
     4021 tgcaattatc gataatggtg tagatattga ttttaaataa tttagaaata aaaataaaat
     4081 aagttatcca agttatatgt atgatgtaat aggggtacaa tgtatagcaa atataaatat
     4141 tggcttaata aaactctact cttaagtcta ctgtcgtttt aacacaagac taataataag
     4201 tgtaacctaa aaattattga taatacaata accttgttta tgataaataa caaatattaa
     4261 gtgttgtaaa gatagtaagt ttaaaaaaat atcaaagatt tcaatcacaa tgtattgttt
     4321 atctgtagtg ttagctattt atggtatata tgtatttata aattgtactg aatatatata
     4381 tactatgcta tctacaaaac atttaacagt caaaggaaat gaatatacca ttataaatta
     4441 ttatatgagt aactttagtc agtatataat atttgctatt ttacttgcat caatgggatt
     4501 tgtaatacaa aagatagtgc atatgcattt cagtgagata gaacttagtg gtaactattc
     4561 aacaactgtt agaaatataa gtgatatctc tttagataat gataatgaaa tgtttaatag
     4621 aggcaatatt gaaaatagta ttttaaaaaa ataaataata taaatatata aaatgtgatt
     4681 gaataaagct tagaatactt agtgtatttt aagctttttt tattattaag atttcaaagt
     4741 attaaattaa ctatataaaa tcatataaaa ataattttaa aatatttaaa tcattgattt
     4801 caaggtattg tatttttaaa agagtaaaaa tataataagt taggtatata atatatataa
     4861 agtaaaaaat aaaaaaatgg aagtgattga gatgagtagt atattgttgg ttgaagatga
     4921 tttaagtttg atatatggat tagaatattc tatacaaaag aatggattta gtgttgatgt
     4981 agcaagaaca gtagaagaag cattgcaaat ttataaagaa aaaaattatg atttactgct
     5041 tttggatgta tccttacctg atggtgatgg ttttgatatt tgtaaaaggg taagagaagc
     5101 ttctaatgta ccaatcatat ttttaactgc ttctgatgaa gaagtaaatg tagtgatggg
     5161 acttgatatg ggtggagatg attacataac aaaacctttt aaattaaatg agttaatttc
     5221 aagaataaaa gctttactta gacgttataa ttttacaagt gaaaatgtaa ctgaactaaa
     5281 atcaaataat attactgtta aattacttga aaatcgtgtt tttaaaaatg aaattgagct
     5341 tgaacttact acagcagaat ataaattatt atgcttactt atgaaaaata aaaatattgt
     5401 acttacaaga aaaaatatat tggataagtt atgggatgga aatggtagtt ttgtagatga
     5461 taatactctg tcagtatatg taagaaggtt aagaaataag attgaagata atcctgaaaa
     5521 tccaaaaaat ctattaacag ttagaagaat gggttacaaa tggaatgtta taaagtgagg
     5581 gtgttaatat gaaggtattt tctaataaag acataaaaat attttttttg gtgattatat
     5641 ccgttttatt gatgtttata gtagtaggtc aaatagtagt tataaatata actaatgatt
     5701 ataaaagtgc actgttagaa catgattata atatagctgg atacttaaat agtatgggtg
     5761 ttgataaatc aaaaatacca gcagtattta ctactaatga agataaatat actttaaaag
     5821 gaaaagaaat cttaaataca gctagatatg actttggtac agatgatagt tttatgccaa
     5881 gtgtgaaatt gtttcaaggt aaatatataa aaactatgtt tttgtattta tttgtgttct
     5941 ttactatgat tatttttata ttgtatgtgt atttttttag gcaacagaac aagatagaag
     6001 aagctagtta taaaattgat tcatttatga atggaaatat aaatgtacgg ttaaatactt
     6061 atgaagaagg tagtttatca aagttatttg gttcaataga tgctatgtca acatctctaa
     6121 acacacatat attgaaagaa aagcagaata aagaatttct taaaaataca atttcagata
     6181 tatcacatca actaaaaact ccacttacag cattgatgat gtataatcaa atattgcagg
     6241 aagagagtca taatagtgaa attgttgaga attttgtgca gaagagtcaa aatgaattag
     6301 agcgtataga atcattgata caaaacttgt taaagattac aaagatagat tcaaagacaa
     6361 taattcttaa caaagctagt gttaacgtaa aaaagttgat tactaaaatt ttatatagtt
     6421 ttgaaactag agcagaaaaa gaaggaaaga gtatctctat aagaggtcta gaaaatacaa
     6481 acttgttttg cgattatgaa tggatatcag aagctttaag caaccttata aaaaatgcat
     6541 tagataatac aaaagagaac gataaaataa tgatagaatg ggttgaaaca cctataacaa
     6601 ctattatttc agtgagtgat accggaaatg gaattcatat tgaagatata catcatatat
     6661 ttaaaagatt ttataggagt aagttttcaa aaagtaatca aggccttgga ttagggttgc
     6721 cactagttaa atctatcata gaacagcaca atggaaccat aactgtagaa agtaattttt
     6781 tacaaggaag tacatttact ctaagttttt taaagcttac taatatgtaa gataggatac
     6841 atcttacagt aaggtgtatg aattattctt atattaatca tcagaaagga gatagagcaa
     6901 atggatattc ttgtggttaa taatttatgt aaaacatatg gaaatggata tacaaaagta
     6961 gaggcattaa aagatgtatc atttacagtt tcaaagggag agtttgttac tgtagttgga
     7021 gaatctggct caggtaagag tacattatta aatcttttag gaggtcttga tactccaacc
     7081 tcaggaaaaa tatttgttga tggtaaagaa atatgttcaa tgaaagaaga aaaacagact
     7141 atttttcgac gtcgtaatat aggttttatc tttcaatcat ataatcttat acccgaatta
     7201 actgtggaac aaaatataat atttccttta ctactagatt acaaaaaacc agacactatt
     7261 tatctagatg aagtattgac aattttaggt cttaaagata gaaaaagtca tcttccaaat
     7321 caattatctg gtggtcagca acaaagggtt gcaattggaa gagcattgat tacaaagcca
     7381 tcaattatat tggcagatga gccaacagga aatcttgaca gtaaaaatag cagtgatgta
     7441 atttcacttt taaaagtgac agcagaaaaa tataaccaaa ctattattat gataactcat
     7501 aatacaaata tagcttctac agcagatagg atactacagg tttctgatgg aatgcttaaa
     7561 gacctagggg tgaatagaga atgagaagct atttagaact agtaccacaa tatgcaaagg
     7621 tacataagaa aaaaaataga actacaattt tatgtattgt cattgcagta tgtttagtta
     7681 ctgctatttt tggactagca gacatggcta tacaatgtca aaaagtacaa tttataaaag
     7741 atgatggaaa ttggcacata gtacttaaaa atcttaatga agaagctata tcagaaataa
     7801 gtagtagagt agatatagct cactatggta gaggacaagt cattgataat ggagagttta
     7861 aatcaaagga gattttgata caaggtttag atgaagattt agcaaaacaa ttaggtctag
     7921 aaataataaa aggtcgttat cctgttgtgg agaatgagac tttaatagat gtagatgcag
     7981 ttaaaagctt taatctaaaa gtaggagatt ttatcaaaat aaaattagca gatggtagaa
     8041 ataaaaatta taagatagta ggtgaatatt catcattatc aagattaaga gcctcagata
     8101 agcatggatt aatgctttca atattaggta cacaagctat caaaaaatca gaaacatatg
     8161 attattatta tattacattt aaagacaagg ttaatattag aaaagctact tctgatatta
     8221 agaatacctt caatttaagt gataaacaaa tcggtaaaaa tgaaaaatta ctttctatta
     8281 taggtcaagg tgactctgat tattctaaac cactttactt aacagcagga ggacttttta
     8341 tacttgtgtt aatagcaagt attatcatga tatataatag ctttaatatg ggtgtaatag
     8401 agagaataaa gttttttgga ctattacgtt gcttgggagc atcaaaactc caagtcaaaa
     8461 aatttgttgt tttagaaagt ttaattctaa gtgtaaaggc tattccacta ggattgttat
     8521 taggatgtat tgtaaccatt atatcctcta tatttctcaa atatgtgaat acagagttat
     8581 ttggttcgat gccaatttta aaggttagct ttattggtat tgtgtttgga ataatagtag
     8641 gatttttaac agtaatatta tcagctattg ttccagcaaa aaaagcttct ggggtgtctc
     8701 cactaagtgc tatacgtggt aatctaatgt tcaaagaaat tttaactagt ggaaaagcaa
     8761 gtaaaattgc taacgcagga agttctaaaa ttgatgtttc tatgggaata aatcatgcct
     8821 tttcaagaaa aaaatctttt atacttatga cttcttcatt tgctattagt attgtattgt
     8881 ttttaggttt tagtgtattt atagacttta tgtatcaggc acttaaacct ataaaaccag
     8941 aagctgctca tatatctatt gttgacgctg actttaagtc tgttcttact aaagacgata
     9001 ttaatgaaat aagtaatatc aaaggaataa aaaaagtata cagtagaatg atgacggatg
     9061 tagaggctac atacaataat aaggaggcaa catctcagct aatttcttat gaaaaaaatc
     9121 aatttgactg ggccaaaaag tatctagtaa aaggggaaat caatgaagat aaattagagt
     9181 cagaaaatat cggattggta gaagaaggtc atgggtttaa tattggagat aagatatata
     9241 ttaaaaatgg aaattctaag aaagagatag aggtagcagg tatactttct tcaatttcat
     9301 ttgatgttaa agacaaatat attggaaata ttataacatc tgaaaatact tttactagta
     9361 taacagatat aaaagactat ataataattg atactcaagt tgatgaagat gctcctgaag
     9421 atttaatcca aaatataaga aatgttctag atgataattt agaggttcga gataagcgac
     9481 aaggtaactt agaggctaaa aattcatttt acactatggc agtttttgta tatgggtttg
     9541 tatttataat tgcgattata agtatgttta atatcataaa cagtatgaat ataagcgtta
     9601 caagtagaat aaattactat ggtattatgc gtgcaatagg catgtccaat aagcaattaa
     9661 gaaaaatggt aatagtagaa tcaagtacat atgctgtaag tggctgttta ttggggagtg
     9721 tacttggatt aatattacat agatatatat ttgtatcctt agtaacatta aaattccata
     9781 tagactggca aatacctttt gatttactgt ttatcattgt agtaactatg ataataataa
     9841 ctttgcttgc agtaagaaaa ccaattaaaa agatttgtga attagacata atagaggtag
     9901 taaatgcaca ataaatataa gtagtaattt aaatagaggt agtaatttaa ataaaaaatc
     9961 gaagtaaata tgaaatagaa aatatctttc ataatttact tcgatttttg tgttatataa
    10021 tttactttag ataagttcca tcttcatctt gataaattaa gtctttcttc ataagtactc
    10081 caagagcacg tttaaaattt tcttacttga attaaataag atagaaatat ctttaggatc
    10141 agatttatca ttgaatctca tatatccatc tgaaccttct aaatagctta aaatcttatt
    10201 ttctaaggta tcaagttcat cttttctgtt tcctctagga gaaagtccta atttaccatc
    10261 atcgtatatt tttataacat gtaaattttc aagaacatcc ccagctttta attctgtaaa
    10321 gtattcatta tgaaggatta caccagcata tttattgtca acacatatca tagcagagtt
    10381 gttggtttgg aatccatata caactccatt tacaacatct cctacattgt aagtatggtc
    10441 tgtttctaaa tatgaatcta tgtctgtagt agcagctatt cttccgcttt tatccaagta
    10501 tatatagaat aaatattttt catctctaaa caatggataa gtcttagctt taaaaggaac
    10561 tagtatgtct tttgtaagtc ccatatctat aaaagtacca atatccgtgt tatcaactac
    10621 ttttagatac gtaacatctc catttctaat tatttctata gtattagaaa aataattttt
    10681 aaatagtttg taaaatatta ttgaattttt tctctcgaaa tatataattt agaatcttct
    10741 gatttatttt tatgctcgat ttccttattt tttttcataa atatatatag aggagctatt
    10801 aacaacattg cccctaatcc tattaaaact ctattttgag ttataggttt tgaaatatct
    10861 tctgcccaag cttgttgtaa caaccataag ctacctaaaa tagctaccat tggtataaca
    10921 ggaccaaatg gaatcttaaa agaggcctta acatctgttc tttttcttaa aactattact
    10981 gataaacaag ttggtatgta ctgagcaaat cttgcaatta cactcattac tgcaagttgt
    11041 tcaaaacttc cagttagcac aagtggtata caacaaatta aagatattat tattgctaca
    11101 tatggagcac catatttatt tgtttttcct ataaatgcag gaagagaacc ttcttctgct
    11161 agagaagaac cacatttagg agttactatt gatgaaccta tatttatacc aaatatagat
    11221 attaatgttg ctacagatat gaaaaattta ccataattgc ctaaaaatac acttgaagca
    11281 tctgcaattg gaatactgtt ttcaaatagt ctatctccaa gtatacccat acagacaatt
    11341 tgaattaata tataaattat tgcacatata aatattgtgg ttattaatgc gactggaaga
    11401 ttttttttag gattttccat ttctccactt gccacaataa atgattcaaa gccggtaaat
    11461 gcgtaaaata ctaatataat ggctggaccc atactagagt taactacatt tcctgaagga
    11521 acaatattta caaattttat aaagaatatt ccaactatta taaaaactat taatggtact
    11581 aatttagcta tagttattac attattcatt atctttgtgc ttttaattcc aaataagcta
    11641 tttattgaaa gtagtgttac gagtattgca gctatataac ctttgtattc agtggcagat
    11701 tctggccaaa atgaacctaa agctgttgca aatcctactg ctagagtaga ccatgatata
    11761 attctaataa cccaagacat tgtaccaact tcaaatccta taaaatctcc aaaagctttt
    11821 ttggagtata gatatgctcc tccattctta tcaaacatgc ttccaacttc tgcaaaacat
    11881 aaaagtattg atagaactaa gagtgtagca aaaatgtata taaacatact gttttgacct
    11941 gctagattat aaactttacc tggtagtaag aatatacctg aacctattat gctattaatc
    12001 cctaataata tcatactaaa tagacctaat ttcttagctt tttccatatt aatttatctc
    12061 ctattctttt attaattact gtactaatta aaatacaaag tattcattac atgcatcata
    12121 acacctattg gtaaacaatc ttcatctata ttaaagctag aggtatgaag aggtgaaatt
    12181 aagccctttt cctcattttt acatcctaaa tggaagaatg ctcctttaca atgttctgtg
    12241 taaaaagaaa agtcttcacc acctaaagac gggtttggtc tcaatataaa tttttcctct
    12301 ccaagtaatt ctttagtatt tgatataact gtatctacta attctttttc atttataaca
    12361 gcaggatagt tttcatcact tacatgtaaa gtcccaacac aaccaaatgc agatgcagtg
    12421 tctccaacaa ttttagctat tttatcaatc ataaagtttc ttgttttgct atttaaggtt
    12481 cttaaagtgc cttctagttt tacatcttca catattacat tgtgtgcatc accaccatat
    12541 attttaccta tagtcaaaac agcagcatta tttggttcta agtttctact tattatagtt
    12601 tgtaatgatg taacaatttg tgaagctgta actattgcat caataccatt ttcaggatat
    12661 gcaccatgag ctcttttacc tttgatactt atttttatag tgtctacact agcatttaat
    12721 gtatcatatt ttatttcgat aaatcctgtt tcgatatgtg gcataacatg aagtccgaat
    12781 atataatcga ccttaggatt ttctaaacat ccatcttcaa ctaagaattt tgctcctcca
    12841 aaaccttctt ctgctggttg aaataagaat tttacattta cattaagttt atcttttatt
    12901 gaatgtaaaa ctttacatgc tccaagtagc atggttgtat gtgcatcatg gccacaagca
    12961 tgcatttttc cagaatgtat tgatttataa ggtatgttgt tttcttcttc aataggaaga
    13021 gcatccatat ctgctcttat acaaatcgtt ttatctgcac tttcttttaa aatatatgcc
    13081 ataattccat tgtgttttgt aaattccata taatcaattc cgatttcttt taaataagaa
    13141 attaccttct gtttagtaag attttcttca agagctaatt ctggaatttt atgtaattct
    13201 cttcttatgt ttataagcca tggttttata gaattacaac tttctattac aaaatctttt
    13261 cctaattctt tggtttgata ttttaatttc aaagcctgtt gcatttttat tcctccaatt
    13321 gctttttatt gttttattaa tataataagc aattattatg ccataatttt cgatggagaa
    13381 ctttgttttt tataagaata ttgaataaat ataatcattt atctatatgt atagtataaa
    13441 aaatgattgc atagtttcaa ttttcagtaa aaaatattat tttgaagaaa atatatttta
    13501 tatgctagtt tatataaa
//
LOCUS       DAYAPA010000121        13162 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.123, whole genome shotgun sequence.
ACCESSION   DAYAPA010000121 DAYAPA010000000
VERSION     DAYAPA010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13162
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.123"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(224..601)
                     /locus_tag="ACGI59_003094"
     CDS             complement(224..601)
                     /locus_tag="ACGI59_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFG9740870.1"
                     /translation="MSKVVIATEKAPGAIGPYSQAIQVGNIVSTSGQLPIDMKTGELV
                     SDPKEATKASLDNVKAILEQAGTSMDKIFKTTVFVKDLKDFNDINEVYATYFKENPPA
                     RSCVQVAQLPKDAVLEIEAMAVL"
     gene            1506..1991
                     /locus_tag="ACGI59_003095"
     CDS             1506..1991
                     /locus_tag="ACGI59_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9740871.1"
                     /translation="MSKSELTAETTEEMLEVLSGKDYDIACHLHELGKSLDCKIEPKT
                     GARSYKIVYSTKKPKRSLFTIECNEKKWRVKANLFHLNTYKDAVEECSKTIKDSITKT
                     RTCKKCNSKCIGGSSFELDGKSYLTCIGSGHYFANMEEMDWKNLEKLITKENNIMQES
                     V"
     gene            complement(2191..2730)
                     /locus_tag="ACGI59_003096"
     CDS             complement(2191..2730)
                     /locus_tag="ACGI59_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HFG9740872.1"
                     /translation="MSKNVLIVSASPRKGGNSDLLCDQFIQGVLESKNQVEKIFLKDK
                     DINYCTGCGACYEKEANCSQTDDMGEILEKMIEADVIVLATPVYFYTMNAQMKTMIDR
                     TCSRYTEICDKDFYFIVTAADENVADMERTIEGFRGFTSCLNGSKEKGIVYGVGAWNV
                     GDIKGSKAMKQAYEMGKLA"
     gene            complement(2723..3136)
                     /locus_tag="ACGI59_003097"
     CDS             complement(2723..3136)
                     /locus_tag="ACGI59_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFG9740873.1"
                     /translation="MTISEVSKKYELSADTLRYYERIGLIPPVNRNKSGIRSFTEEDC
                     EWVNFIKCMRGAGLSIETLIEYVAMFQQGSSTIKARKELLIEQRNQLAKRIEEMQKTL
                     ERLNFKIDRYEEGIIEKEKVLKSSRNKKMEAVSYE"
     gene            complement(3172..4293)
                     /locus_tag="ACGI59_003098"
     CDS             complement(3172..4293)
                     /locus_tag="ACGI59_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFG9740874.1"
                     /translation="MEAKKLGFGLMRLPIKDENDVTTIDMEHLNKMVDTFLERGFTYF
                     DTAYVYHMGKSEIALRESLVKRHKRESFTIATKLPLMALKKKEEQETIFNEQLEKCGV
                     DYFDYYLLHNIGVSHYEVAKKFDSFKFIEEKKKEGKIKNIGFSFHDSAELLDKVLSEH
                     PEVDFVQLQINYLDWDNESIQSRKCYEVAEKHNKPVIVMEPVKGGTLAKIPEKAEKLL
                     NDYNPDMSISSWAIRFAASKDNVMMVLSGMSNMEQLLDNTGYMQNFKPFVQEEYNIID
                     EAVEIINESILVPCTACQYCVEGCPKNIAIPNYFALYNAEKQALNTGFSTQMVYYNNY
                     TKTYGKASDCIECKQCEESCPQHIKIIDALKNVAETFEK"
     gene            4881..6533
                     /locus_tag="ACGI59_003099"
     CDS             4881..6533
                     /locus_tag="ACGI59_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp21"
                     /protein_id="HFG9740875.1"
                     /translation="MNKKNLAILISGIMFFSSSSAIFADNTTKQERLIGKTRYETAVE
                     VSKLGWVQSKTAIIVNGNSIQSALCANPFAKLKNAPILLVNNNSIENSTKAELKRLGV
                     DNVYIVDSGNSISSKVENEIKSLNIKINKIVGNNIYEMSTNVLKEIDKIKKVENVAVV
                     NGLKGLSDAVSIASPAAIHNMPIILVSDENSNDSSINFLNNRSIKKSYIVGGNKILSD
                     NTVKKYPNRERISGSDRNNTNAKIIDKFYKDKKINNVFVAKDGINQENALVDSLSIGV
                     LAAKENSPIMISNGSLVNSQKEFIKNKDIDEITQVGGGYNQKAFEDILKLKNVNIRNK
                     TKKISLIQAKEILKQALPGSNILVCYYDEDDNEYDCKVIKDNILYEIEIDAYSGKIIE
                     IEKDDDLNDNNLGTQNISLDKAKSIALDAVPNGNLIYCKYDAEDNEYEAKVIKNKKTY
                     ELEISAFDGRIVDIDMDNDSDDDFDDNDKNNSNTNDNSSIISAEKAKSIMLDKVPGGK
                     IVKFKYDEDDKEYEGEIIKGKTEYEITINAITGKILEFEIDD"
     gene            6832..7581
                     /locus_tag="ACGI59_003100"
     CDS             6832..7581
                     /locus_tag="ACGI59_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005340256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9740876.1"
                     /translation="MNTILKVNNITKTYNKGQQPSLDKVSFNVSEGEFIGIMGASGSG
                     KTTLLNVLSTIDKPTSGDIFINNINLKSLNDTCAADFRKDNLGFIFQEYFLLDSLTIF
                     ENISVPLTLQKLNPDKIENMVKSLAERFGIASQLNKYPNELSGGQRQRASAVRAIVKH
                     PSILFADEPTGALDSNSATELLHSLQEANEELKTTILMVTHDAYVASFANRILIFKDG
                     HIIKELNKQQNNRHTFYELIIQEIAKLDTKR"
     gene            7594..9621
                     /locus_tag="ACGI59_003101"
     CDS             7594..9621
                     /locus_tag="ACGI59_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFG9740877.1"
                     /translation="MNIFYTAIKNLKKNFSFYSLYLLSVSFVITVFFAFTSFSVNKIM
                     LEKISTDGRVESMCNVISIFLMAFVIFYMAYSNRFFLRRRTKELGIYALLGYRKFTIL
                     SLLTFENVLICSGAFIIGLVLGALAHKGIVFGITALLNLSIDNSQIPFFNINAINKTI
                     IFIALVVLVLAVSNGKFFYKTPLVKLIRYEKSAEKKLKFRKIPAFMGFIMILSGYCLS
                     LDILRGAKSVWVSIGFYQMGLITMIIIVIGTVLFINSFLPYIMQNSKKNKRTFYTDMK
                     IIAIPNFIYRIRSNSKTLIMLTLLSATTLTVSSVMALSLYYPIASVSRIAPSEIEFRV
                     EKNKQIEDVKQIVSQYTSNTDDIIFTQTNIYKVKSSSKDLPFEYEIGTSKNNLSNSSS
                     SKEVISRESGFECISFSDYYTLLKAQNRTKLLKQLTPLRDNECILMKYQPNNDGYSEN
                     GTIYPLLVNGKETPVAVRETSLNNAISFANSVGTLIVNDSIYEQIAQNKLPMTRILSI
                     NGKSIKNNENLYSNISDYLDGSPYLQGYSHRINDLLSINSSTFLLIGFLVVLFFIAVG
                     SILYFNNISAISDSTSDYKILQKMGYTNIQIKKIIKKQVLPFFNIPFLLGIVDCIFAT
                     LVYKTALMQNLLGNSLSQYIPVIIAIFLTSVVYLIYYILTIKSCCKIVLKK"
     gene            9765..11348
                     /locus_tag="ACGI59_003102"
     CDS             9765..11348
                     /locus_tag="ACGI59_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFG9740878.1"
                     /translation="MTNFNLKILFLGNYILSLLFMGISTSTETYMNIQSIIPLIISTL
                     LLIDITYITSCNVRENNIISLFCGLLALDSWYLLLSFVTTPIGTIIFKILSTVIVYVS
                     IKFIFLFLFQGSGYNFRKITDILLLITCIGAIVGAFISNRTYACMYGIQFVVSIISFF
                     YIIFYHLKRVVFVIKSEKKPISISVFITIIGFLTYYFFTLRISNHIGNFGIYLTVLVF
                     FMSIHGIALKENDSFPISTVFNIKQRAIFLFISILVISLVVLFANGNFSIILIAINVL
                     FMLIFLCNIILEFNLRDEKNKVVKNSKYTFALEKLQQEEQLKTEFSNFLHDDILQNLL
                     SIKNMMSKSYRPDVQDIIYETLNNLNTCIRKQMQDYHPVILKNLTVKENYQNLIESVS
                     SSFPQKNICVSFKCSDTLFLVEPYDILIYRLIKELLTNVYKHSNGSHAWIALALEKNI
                     IKLNISDNGVANTSFKTPLDITKITFDTIKHKGIISIKEQVENLGGTISVSDNIPHGV
                     CVQITIPMKGDGSYKYFVS"
     gene            11320..11940
                     /locus_tag="ACGI59_003103"
     CDS             11320..11940
                     /locus_tag="ACGI59_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFG9740879.1"
                     /translation="MVLINILLVDDHMLFAKSLSIALEEYKEIEQFYSTQDIHNLSEM
                     IQDKKIDIVLMDINLRGLSECDGLKVAYNLLETNSTIKIIILTGYDLPVYKHEAKKIG
                     IYGFLNKNISPDSLIDSLLKVYQGVTCFPSENTEVIIEDLTTMEKRILQLICSGKKRK
                     IIADELYISERTLSNHLQHIYDKLDVSSSVEAITKAIKMGYISPVY"
     gene            complement(12129..13055)
                     /locus_tag="ACGI59_003104"
     CDS             complement(12129..13055)
                     /locus_tag="ACGI59_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conserved phage C-terminal domain-containing
                     protein"
                     /protein_id="HFG9740880.1"
                     /translation="MSNSFYQLNSAVLYFNEKRDVNRIRFNHYVMRKENIQRLKGTLP
                     RGQFMMTVRKAASDLDVSISTISRLVNEFIDLGILRLISRGVKGNCCSVYSYVCSESS
                     NAEIVTKNGDIFKNIEKYIDATINNNQKTMKYKHRNELFKNSNTNIYSRINQESIDKD
                     YDLIIHTGYETENETKKKELLKNNLNKKNLNKNTFENSDEDLTEDSTYKNPESIHEII
                     IRKLNSESGKNFKADLSITKALIDKRLKEGYSLEDFYKVIEAKVRRWRYTDMEIYIEP
                     TILFGYEFESYLNEESSLSACNYGDMCYEDMY"
BASE COUNT         4777 a         1876 c         1682 g         4827 t
ORIGIN      
        1 agacaataaa ataaatcaca atacaataat aaaaaaaagc tttcctcata taacaaatat
       61 atttttgaca taaaaatata cattatatga gaaaagctca atagttaaat atttagatag
      121 atttttatat agactttttt aatataaact tttttaattg tagacttttt ttattagttt
      181 ttaattttta ttagttttgt tattggtttt attgttataa tacttataat actgccattg
      241 cttcaatttc taaaactgca tcttttggta attgagcaac ttgtacacat gaccttgctg
      301 gtggattttc tttaaaatat gtagcatata cttcattaat atcattaaaa tcctttaaat
      361 cttttacgaa tactgtagtc ttaaatattt tatccatact tgtaccagct tgttctaata
      421 ttgcttttac attatctaaa gaagcttttg ttgcttcttt tgggtcagat actaactcgc
      481 cagtcttcat atctattgga agttgacctg atgtagaaac tatgtttcca acttgaattg
      541 cttgagaata tggtccaatt gctcctggag ctttctctgt agctattact acttttgaca
      601 tgacttcacc tcctattatt aagttatatt tacaggaaaa taaaatttcc taaacaccaa
      661 tattaatacg aacctttaga ttaaacttca aatctaaaac ttcaaataat caatacttag
      721 atgattatgt ttatttgtgt cttgttaact tttattaaag caattaacgt gccaaacttt
      781 atttatatat aaaattttgt gaagtcattc aaaatagtta cttctgaatt ttttcgtaaa
      841 aaaacgctct aaaattactt ttttatgatg tcaatttttt tctcatatct agaaatttac
      901 caacatgaga attcttgttt tgagaattta cagattaaag ttatttttta accttaaatt
      961 ctgaacctcc catgactaaa gtcacagggt tcctgcttca tagaattttg cataatcaag
     1021 ttattacata tgaaagaact atagttgaca atcccactat tataaataat aaaaccttta
     1081 cccatacctt atttacccta gttatcatag agcctattga aaatataaca gctaaatata
     1141 ataataacca aaaaattatc aatatctacc actgttgctg aaaatccaaa aaactacctt
     1201 ttgatagtat tacttaacaa tctaattaat ctcataataa atttacaact tataaataaa
     1261 ttgttatatt ggccgtatct caacaacttt ctcctttcct agttttaaat cttttataga
     1321 attgtaccac gtctaagtaa attttcatac tattaagtct tatttctaat aaatcattag
     1381 ctctcattct atgcccaata atgtaagatt gcagtaattt tattgatttt aatagtttta
     1441 tttaatatac ttatattaat tattttcata tgaaattatt atttatatag ggggattttt
     1501 ttaatatgag caaaagtgaa ttaacagcag aaacaacaga agaaatgtta gaagtactaa
     1561 gtggtaaaga ttatgatatt gcatgtcatt tacatgaact tggtaaatca ttagattgta
     1621 aaattgaacc aaaaacaggt gctcgttctt acaaaatagt atattcaact aagaaaccaa
     1681 aacgtagctt atttactatt gaatgtaatg aaaaaaaatg gagagttaaa gcaaatcttt
     1741 ttcatctaaa tacatataaa gatgctgtgg aggaatgctc taaaactatt aaagatagta
     1801 taactaaaac tcgtacttgt aagaaatgta attcaaagtg tattggaggt tcttcgtttg
     1861 aattagatgg aaagtcttac ctgacttgca taggaagtgg tcattatttt gcaaatatgg
     1921 aagaaatgga ttggaaaaac ctagaaaaat taattactaa agaaaataat attatgcagg
     1981 aatctgtata gttttttaag ggtaattatt aaaatatatg agtaacaaca gatacttaaa
     2041 taggcataaa aaaataaagg gtataataag gatatagaat aaaaataaat gatatagcac
     2101 aaagaggctg tctcaaaata cagataagcc tctttttatt gttgaaatga taagaatata
     2161 ttcaatttta tacaatttta tacaaacata ctacgccaac ttacccattt catatgcctg
     2221 tttcattgcc ttacttccct taatatcacc tacattccaa gcacctacac catatactat
     2281 acctttttcc ttagaaccat ttagacatga tgtaaatcct ctaaatcctt ctattgttct
     2341 ttccatatca gccacatttt catctgctgc tgtaacgata aaatagaaat ctttatcaca
     2401 aatttcagta tatctagagc aagttctatc aatcatagtt ttcatttgcg cattcatagt
     2461 ataaaaataa actggagtcg ctaaaacaat cacatctgcc tcaatcattt tttcaagaat
     2521 ttctcccatg tcatctgtct gtgaacaatt tgcttctttt tcataacaag caccacatcc
     2581 agtacaatag ttaatatctt tatcttttaa aaatatcttc tccacttgat ttttagattc
     2641 taatacccct tgtataaact ggtcacacaa taaatcagaa tttccacctt ttctaggact
     2701 agctgatact attaatacat ttttactcat aagatactgc ctccattttc ttatttctac
     2761 tgctctttaa tactttttct ttctcaataa taccttcttc atatctatca atcttaaaat
     2821 ttaatcgttc caaagttttt tgcatttctt ctattctttt tgctagttgg tttctctgct
     2881 ctattaaaag ttcttttcta gctttgatag tactgctccc ttgttgaaac atagctacat
     2941 actcaattaa tgtctcaata gaaagacctg cacctctcat acactttatg aaattaaccc
     3001 actcacaatc ttcttctgta aaagacctaa ttccactttt atttctattt acaggtggta
     3061 ttaaaccaat tcgttcataa tatcgtaaag tatccgctga aagctcgtat tttttactaa
     3121 cttctgaaat cgtcatacat atcttctcct cttgatattg tatagattga attatttctc
     3181 aaaggtttct gctacattct ttaaagcatc tataatttta atgtgttgag gacagctttc
     3241 ttcacattgt ttacattcaa tacaatctga ggcctttcca taagttttag tataattatt
     3301 atagtagacc atctgtgttg aaaatccagt attaagagct tgtttttctg cattgtacaa
     3361 cgcaaaatag tttggaatag cgatattttt tggacagcct tctacacaat actgacaggc
     3421 agtacaagga actaaaattg attcgttgat aatctctact gcttcatcta taatattata
     3481 ttcctcttgt acgaatggtt tgaaattttg catatatcct gtattgtcta ataattgttc
     3541 catatttgac atcccactta agaccatcat aacattatct ttacttgctg caaaacgtat
     3601 tgcccaagat gaaattgaca tatctggatt gtagtcattc aataatttct ctgctttttc
     3661 tggtatcttt gcaagtgtac ctccttttac tggctccata acaattacag gcttattatg
     3721 cttctctgcc acctcgtagc attttctaga ttgtatgctt tcattatccc aatctaaata
     3781 attaatctgt aattgcacga agtctacttc tggatgctca ctcaagactt tatccaataa
     3841 ttctgcacta tcatgaaaag aaaaaccaat attctttatt tttccttctt ttttcttttc
     3901 ttcaataaac ttaaagctat caaatttttt agctacttca taatgtgaca cgcctatatt
     3961 gtgtaaaaga tagtagtcaa aatagtctac accacatttt tctaattgct cattaaaaat
     4021 ggtttcttgc tcttcttttt tctttaaagc cattaatgga agttttgtag ctatagtaaa
     4081 actctctctt ttgtgtcttt ttactagaga ttctctaagt gctatctcac tttttcccat
     4141 gtgatacaca taggctgtat caaaatatgt aaatcccctt tctaaaaaag tgtctaccat
     4201 tttatttaaa tgttccatat caatagttgt aacatcattt tcatctttta ttggtaaacg
     4261 cattaatcca aatcctaatt tttttgcctc cattgtttta cttccccctt taaaacttgt
     4321 ttgttttttc tctctacttt aactattctt acaagtatat aattcttgta taattatact
     4381 ttactacttg gagttaactt caagtcaata gattttttta tttttaagac aaatattata
     4441 aaaatacagt tctttaatat taatccatta catgtttctt tcaccctcca attctcaaac
     4501 ttcacaaact ccaacatgta aagaaatata tactttaaga aattcagtcc attaatatta
     4561 ctacaaatac tttttagata tgtttaaaaa gaaatttcac ctaaaagatt tagaaatagg
     4621 tcttgtgtct attttaaaat atataaaaca gtggtaaaga cttatttata agataaaaaa
     4681 tgaatagtgt ataccctttt taaaaagttt tttccttttt atatttgtga tacaatgtta
     4741 aatataaaaa ggaaaatttt gtattaaaaa aatcttattt gttgattttg taacttcaaa
     4801 ctcagcatca atttgatttt aagattatat tttattaagt atgctaaata acaaattttt
     4861 aaagattgtg aggtaaaaat atgaataaga aaaatttagc tatattaata tcaggtatta
     4921 tgtttttttc tagttcttca gcaatttttg cagacaatac tacaaagcaa gaaagattaa
     4981 taggtaaaac aagatatgaa acagcagtag aagtaagtaa attgggatgg gttcaatcaa
     5041 aaacagccat tatagttaat ggtaattcta ttcaaagtgc gctttgtgca aatccatttg
     5101 ctaagctaaa gaatgcacct atacttttag taaacaacaa cagtattgaa aattcaacta
     5161 aggctgagct taaaaggctt ggtgtagata atgtgtacat tgtagatagt ggaaatagca
     5221 tttcttctaa agtagaaaat gaaataaaat cacttaacat aaaaataaat aaaatagtag
     5281 gaaacaatat atatgaaatg tctacaaatg tacttaaaga aatagataag ataaaaaagg
     5341 tagaaaatgt tgcagttgta aatggactaa agggactatc tgatgcagtt agtatagctt
     5401 ctccagcagc aatacataat atgcctataa tattagtatc agatgaaaac agcaatgata
     5461 gtagtataaa tttcttaaac aataggtcta taaaaaaatc ttacatagtt ggaggaaata
     5521 agattttatc agacaataca gtaaagaaat atccaaacag agaaagaatc agtggttcag
     5581 atagaaacaa tactaatgca aaaattattg ataaatttta taaagataaa aaaataaata
     5641 atgtatttgt agctaaagat ggtattaacc aagaaaatgc attagtagat tcattatcaa
     5701 taggagtact agctgctaaa gaaaattctc ctataatgat atctaatggt agccttgtaa
     5761 atagtcaaaa agaatttatt aaaaataaag atatagatga aataacacaa gtaggaggag
     5821 gatataatca aaaagcattt gaagatattt taaaactaaa aaatgtgaac ataagaaata
     5881 aaactaaaaa aatatcttta atacaggcta aagaaatatt aaaacaagct cttccaggaa
     5941 gcaatatttt agtttgttat tatgatgaag atgataatga atatgactgc aaagttataa
     6001 aggataatat cctatatgag atagaaattg atgcctactc tggtaaaata atagaaattg
     6061 aaaaagatga tgacttaaat gataataatt taggtacaca aaatatatca ttggataaag
     6121 ctaagtctat agcattagat gctgttccta atggtaattt aatctattgt aaatatgatg
     6181 ctgaagataa cgaatatgag gctaaagtaa taaaaaataa gaaaacatat gaattagaga
     6241 taagtgcatt tgatggaaga attgtagata ttgacatgga caatgattct gatgatgact
     6301 ttgatgataa tgataagaat aactctaata ctaatgacaa cagttctatt attagtgctg
     6361 aaaaagctaa atcaataatg ctagataaag tgcctggtgg gaaaatagta aaatttaagt
     6421 atgatgaaga tgacaaagaa tatgaaggtg aaataataaa aggaaaaact gaatatgaga
     6481 taacaattaa tgcaattact ggtaaaatat tagaatttga aatagatgat taataaaaaa
     6541 tatatattct attaattatt acaagcaata aagccataaa tattgaaaat ttcaatattt
     6601 atggctttat tttatttttt ttaatttaaa tccacattat gccattatat atacctatgc
     6661 actcttttaa attaaaatct tgtccatcat cccaaaccta ataaagttag attttaaaca
     6721 atatattgtg atataagtaa tttgtgtaga attattgcgt gatttactca tgaagaaaca
     6781 ataaggttaa tatataattt gactatatta agacgaaagg agacatatac aatgaataca
     6841 atattaaaag taaataacat aactaaaact tataataaag ggcaacagcc gagtttagat
     6901 aaggtatcct ttaatgtatc agaaggtgaa tttataggta taatgggagc ttctggctct
     6961 gggaaaacaa ctctacttaa tgtattatct acaattgata aaccaacaag tggagatatt
     7021 ttcataaata atataaattt gaaatcgctt aatgacactt gtgctgctga ttttcgtaag
     7081 gataatttag gctttatctt tcaagagtat tttttactag atagtcttac aattttcgag
     7141 aatatttcag tacctcttac acttcagaaa ctcaatccag ataaaataga aaacatggta
     7201 aagagtctag ctgaacgatt tggcatagct tctcaattaa ataaataccc aaatgaatta
     7261 tctggtgggc aaagacagag agcttcagca gttagagcaa tagtaaaaca tcctagtatt
     7321 ctctttgcag atgaaccaac aggagctttg gattctaatt cagctactga actactccac
     7381 tcactacagg aagcaaatga ggagttgaaa acaacaattt taatggtaac acatgatgcc
     7441 tatgtagcaa gttttgcaaa tagaattttg atttttaaag atggtcatat cattaaagaa
     7501 ttaaataaac aacagaataa tagacataca ttttatgagt tgattattca agaaattgca
     7561 aaattggata ctaaaagata ggaggataat ttaatgaaca ttttttatac ggcaataaag
     7621 aatcttaaaa aaaacttttc attctattct ctatatctac tttcagtctc ttttgttatt
     7681 actgtatttt tcgcatttac ttcattttct gttaataaga taatgctaga aaaaatttca
     7741 actgatggta gagttgaatc tatgtgtaat gttatttcga tatttttgat ggcatttgtc
     7801 atcttctata tggcatactc aaatcgattc ttcttgagac gtcgtacaaa agaattaggt
     7861 atatatgctt tacttggcta tcgtaagttt acaatattat cgctacttac ctttgaaaat
     7921 gtattaattt gtagtggagc ttttattatt ggattggtat taggagcatt ggcacataag
     7981 ggtattgtct ttggaattac agcactactt aatttatcaa ttgataattc acagatacca
     8041 ttttttaata taaatgcaat aaataaaaca attatcttta tagcattagt tgttttagtc
     8101 ttagctgtat caaatggtaa atttttttat aagactccac ttgtaaaatt gataagatat
     8161 gaaaaaagtg cagaaaaaaa attaaagttc agaaaaattc cagcctttat ggggtttatt
     8221 atgattttaa gcgggtattg tttatcacta gatattcttc gtggagctaa atcagtatgg
     8281 gtaagtattg gattttatca aatgggcttg ataactatga ttatcattgt aattggtaca
     8341 gttctcttta tcaattcctt tcttccatat attatgcaaa atagtaaaaa aaataaacgt
     8401 actttctaca cagatatgaa gataatagct attccaaact ttatctatcg tatacgttca
     8461 aactctaaaa ctctaattat gcttacatta ctctctgcta ctactttgac agtttcaagt
     8521 gttatggcac tgtccttata ctaccctatt gcatctgttt cgcgtattgc accttcagag
     8581 atagaattta gagtagaaaa aaataaacaa atagaagatg taaaacaaat tgtcagtcaa
     8641 tatacgtcta atacagatga tattattttt acacagacaa atatttataa ggtaaaatct
     8701 tcatcaaaag accttccatt tgaatatgaa attggtacat ctaagaataa tttatccaat
     8761 agtagttctt ctaaagaagt tatttcaaga gaatcaggat ttgaatgtat atctttttct
     8821 gattattata ctttattaaa ggcacaaaat cgaacaaagc tactaaaaca acttacccct
     8881 ttaagggata atgaatgtat tttaatgaaa tatcaaccaa ataatgatgg atatagtgaa
     8941 aatggaacga tttatccatt acttgttaat ggtaaagaaa cacctgtagc tgtaagggaa
     9001 acatcattaa acaatgctat ttcttttgcc aatagcgttg gaacacttat cgttaatgat
     9061 agtatatatg agcaaatagc acaaaataaa ttaccaatga caagaatttt aagtattaat
     9121 ggtaaatcaa ttaaaaataa tgaaaattta tatagtaata tttcagatta tttagatgga
     9181 agcccttatt tacaaggata ttctcacagg ataaatgatt tattatcaat taatagttct
     9241 acatttctgt taatcggatt tcttgtggta ttattcttta ttgctgttgg aagtatttta
     9301 tactttaaca atatatcagc aatttcagat tcaacatctg attataaaat tttacagaaa
     9361 atgggttata ctaatataca aattaaaaaa attattaaaa aacaagtatt gccatttttt
     9421 aatattccgt ttttacttgg tatagtggac tgtatatttg ctaccttggt ttataagact
     9481 gctttgatgc agaatttact tggaaattca ctttcacaat acatacctgt gattattgca
     9541 attttcttaa cttctgtagt atatttgata tattacatac ttactattaa atcctgttgt
     9601 aaaattgtat taaaaaaata atatttgcca aatcgtcaag cttaaaggtg tgacgatttg
     9661 gtatacttca tttataagta tttgatataa tttaaaacta taaattctta agtttgattt
     9721 agtcataaaa ttaatataat taactcataa ggaggtgtat aagattgaca aattttaatt
     9781 taaaaatact atttttagga aactacattc tatcacttct cttcatggga atatctacaa
     9841 gcactgaaac ttatatgaat attcaaagca ttattccctt aataatttca acattattac
     9901 tcatagacat cacatatata acctcttgta atgtccgaga aaataacatt atttcattgt
     9961 tttgtggatt actagcacta gacagttggt atttactctt atcatttgta accacaccaa
    10021 taggcactat tatttttaaa atattaagca cagttatagt gtatgtctct attaaattta
    10081 tatttttatt tttgtttcaa ggtagtggat acaatttccg taagataaca gacattttat
    10141 tgcttatcac ctgcattggt gcaattgtag gagcatttat ttctaacaga acgtatgctt
    10201 gtatgtacgg aatacagttt gttgttagta taataagctt tttctatatt attttttatc
    10261 atttgaaaag ggttgttttt gtcataaaaa gcgaaaaaaa acctatatcg atttcagtat
    10321 ttataacaat tataggattc cttacttatt atttttttac actaagaata tcaaatcata
    10381 ttggtaattt tggaatctat ctaactgtac tggtattttt tatgagtatt catggtattg
    10441 cacttaaaga aaatgatagt tttcctattt caacagtttt taacataaaa caaagggcta
    10501 tatttctttt tataagtatt ttagtaatta gcttggtcgt actatttgcc aatgggaatt
    10561 tttctataat attaatagct ataaatgttt tatttatgct cattttcctc tgtaacatta
    10621 ttttggagtt taaccttaga gatgaaaaaa ataaagtagt taaaaatagc aagtatactt
    10681 ttgcattaga aaaattacaa caagaagaac aactaaaaac agagttttct aattttttac
    10741 atgatgacat attacagaac ctgctttcaa taaaaaacat gatgagtaag tcatatcgcc
    10801 cagatgttca agatattata tatgaaacac ttaacaatct aaatacatgt ataaggaagc
    10861 aaatgcaaga ctatcatcct gttattttaa aaaatttaac agtaaaagaa aattatcaga
    10921 atttaattga aagtgtttct tcatcctttc cacaaaaaaa tatttgtgtt tcatttaagt
    10981 gttctgatac tttattctta gttgaaccat atgatatatt gatatatcga ctgattaaag
    11041 aattattgac aaatgtgtat aaacattcaa atggtagcca tgcatggata gctttagcac
    11101 tagaaaaaaa tataataaaa ttaaatatta gtgataatgg tgtggcaaat acatctttta
    11161 aaacgccttt agatataact aaaattactt ttgatacaat aaaacacaaa gggattatat
    11221 caattaaaga acaagtagaa aatctaggag gaacgataag tgtttctgat aatatacctc
    11281 atggagtttg tgtacagatt acaatcccta tgaaaggaga tggttcttat aaatattttg
    11341 ttagttgatg accatatgct ttttgctaaa agcttgtcaa ttgcactaga agaatacaaa
    11401 gaaatcgaac agttttattc cacacaggac atccataatc tttcagaaat gattcaagat
    11461 aaaaaaatag acattgtctt aatggatata aaccttagag gcttaagtga atgtgatggc
    11521 ttaaaagtag cttataattt attagagact aattcaacta ttaaaataat aatcttgact
    11581 ggatacgacc tcccagttta taaacatgag gctaaaaaaa taggtatcta tggattcctc
    11641 aataagaata ttagccctga tagtttaatt gattcattat taaaagtcta tcaaggggta
    11701 acttgttttc catctgaaaa tactgaagta attattgaag atttaactac tatggagaaa
    11761 agaattttac agcttatctg tagtggtaaa aaaagaaaaa taattgctga tgaattgtat
    11821 ataagtgaaa gaacattatc aaatcattta caacacatat atgacaagct agatgtttca
    11881 tcatcagttg aggctattac aaaagctatt aaaatgggtt atatttctcc agtatattaa
    11941 aaaaagtgag tgtctcaaaa taaatcttct aactcataaa gcactctcct ttgtattaaa
    12001 tcaaatatat tttcatcatt taaacaatta agaagaggct tctctctatt ttgagacaac
    12061 tccattttaa tacactattt atatttatat ataaacatat tttaaacaac taaaaagcaa
    12121 ctaatatctc aatacatatc ttcataacac atatcaccat aattacaagc agataaagaa
    12181 ctctcctcat ttaaatacga ctcaaattca tacccaaaca gaattgttgg ttctatatat
    12241 atttccatat ctgtatatct ccatcttctt acctttgcct ctataacctt gtaaaaatct
    12301 tctaaactat acccttcttt taatctttta tctataagtg ccttagttat actcaaatca
    12361 gctttaaaat tttttcctga ttcactattt aattttctta taataatttc atgtatgctt
    12421 tctggatttt tgtaagtaga atcttcagtc aaatcttcat ccgaattttc aaaggtattt
    12481 ttatttaaat tctttttatt taaattattt tttaataatt cttttttctt tgtctcattt
    12541 tctgtttcat acccagtatg aattatcaaa tcataatctt tgtctatact ctcttgattt
    12601 atacggctgt atatgttggt attactgttc ttaaacaact catttctatg tttatatttc
    12661 attgtttttt ggttattgtt aatagttgca tcaatgtatt tctctatatt tttgaaaata
    12721 tcaccattct ttgttacaat ttctgcatta ctactttcag aacaaacata actatataca
    12781 gaacaacaat ttccctttac tcccctactt attaatctca atatacctaa atctataaat
    12841 tcatttacaa gtctacttat tgtagatata gatacatcaa ggtcagatgc agctttcctt
    12901 actgtcatca taaattgtcc tcttggcaga gttcctttta atctttgtat attttctttt
    12961 ctcattacat agtgattaaa ccttattcta tttacatctc ttttttcatt aaaatataat
    13021 actgctgaat ttaattgata aaaactatta ctcatattcc cactcctcca atttaaatag
    13081 ataatttgtt gtaaagtttt tagattttta aataacttat taaatgatta tttttattaa
    13141 aaagtattct ttgatatttt ca
//
LOCUS       DAYAPA010000122        12887 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.124, whole genome shotgun sequence.
ACCESSION   DAYAPA010000122 DAYAPA010000000
VERSION     DAYAPA010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12887
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.124"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            166..354
                     /gene="rpmB"
                     /locus_tag="ACGI59_003105"
     CDS             166..354
                     /gene="rpmB"
                     /locus_tag="ACGI59_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFG9740881.1"
                     /translation="MAKVCSVCGKGKVSGNQVSHSNKHNKRTWSANLRSVRAIIDGAP
                     KRVKVCTRCLRSGKIERA"
     gene            complement(514..666)
                     /locus_tag="ACGI59_003106"
     CDS             complement(514..666)
                     /locus_tag="ACGI59_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate translocator"
                     /protein_id="HFG9740882.1"
                     /translation="MRNNKSKVLLGILCLATGMAVILSMLLPGWIWSALTALILIGCG
                     ALLFFC"
     gene            complement(764..1240)
                     /locus_tag="ACGI59_003107"
     CDS             complement(764..1240)
                     /locus_tag="ACGI59_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFG9740883.1"
                     /translation="MNLKRSNEKREEYRRMYSDKESFLSLIQNFTSVSIAKELTLKNI
                     ELETSFICEYKGKEVDIIYKVFSKSGKVSHYIVLEFQTEMDTEIVPRLKSYREQIWKS
                     FIMKKSLEEIEDKNFKLPKVIPVVLYSGPERLSRKKGILDIVESISEFSNNSSMKN"
     gene            1459..2220
                     /locus_tag="ACGI59_003108"
     CDS             1459..2220
                     /locus_tag="ACGI59_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFG9740884.1"
                     /translation="MEYTVQKLSKLAGISTRTLRYYDEIGLLKPLKINSSGYRIYGQN
                     EVNKLQQILFYRELGINLENIKNIINSPNFDSLSALKEHHNKLLAKRKQIDLLIENVT
                     KTIALKEGKYTMTDIEKFEGFKEKMIDENEKNYGTEIREKYGKDIIDTSNKKLKNMSK
                     QDYENWQNLNIEIISKLKKAFKTGDASSELAQEVAKLHHKWLCYTWNTYSKEAHSSLA
                     QMYVYDERFTSYYDREQPGLAKFLRDAIVIYTRDK"
     gene            complement(2480..4408)
                     /locus_tag="ACGI59_003109"
     CDS             complement(2480..4408)
                     /locus_tag="ACGI59_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFG9740885.1"
                     /translation="MHKRLLTLFKLLNESDDKITCKTLSNHLKVSERTIRNDITSING
                     TLEKNGAIIKIKKGEGYYIDILNLALYQQYLALISDDIMDSSEIPDSPIERNQYILKY
                     ILYNNTYIKLEDLANSLYVSKFTILNDIKRIKPILSKYNLILVSKPYYGVKVEGKEID
                     IRRCISNNMINRNFENYIIGITDREIELFNNVDLIELQRVVLSEINKFNINFLDFNLK
                     NFIIHLAITVSRILDGYCLDNVLDVVLTDFQSNTTVENIFNYIESKYTIIISKADRVY
                     LYNHFITKSSLLDNVSNRVDTKIIEYVEEILEVINNQYTFDLRNDSVLFDDLVLHFKS
                     ILNSKSYNLNKVNPLINTIKSNYPLAFEITLNAIEKVFKNSIYSLTEDEIGYVSLHIG
                     AGIERFFQSNIKCKNVVLVCGSGYGSSRLLEVQLNKVFHDKINILQCLSFNQFLASEL
                     SDVDIIISTIPLNHDSIPIVLVDLKLLKKDIENISKSITNNSHIYSNLLDNFFDKNLF
                     IVNPKIKDKDELIKLMCNKLTQSEIVFPSFAESVFYRESLSSTNIDDFLAIPHPMELS
                     SIRTKICISILNEPIYWSENSTVKLIMMLAINKDDYIKINSIYDILLKIIHDNDIRDS
                     ISNCNDFDNFLSIIKSIV"
     gene            4642..5094
                     /locus_tag="ACGI59_003110"
     CDS             4642..5094
                     /locus_tag="ACGI59_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFG9740886.1"
                     /translation="MVLQEKYIFIDKDLKSKEEVLNFIADSAYILGITTNKEALLKDL
                     LKRENEFPTGIKDGFAIPHTKSVNVKEASILYIKTTNELEWKTLDDSKVRYVFSLLAP
                     HKNEGNIHLKTLSKLATCLMEDDFTSEVKNASDASHLVKYITNKMKEE"
     gene            5097..5405
                     /locus_tag="ACGI59_003111"
     CDS             5097..5405
                     /locus_tag="ACGI59_003111"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFG9740887.1"
                     /translation="MKIVAITSCIAGLAHTPMAAKALEQAASKMGHEIKVEQQGAMGR
                     VNTITKEEAQDADFILIASDQTISGMERFDGKKVIKVKIGIALKKPEAVLTKCIEAIS
                     "
     gene            5519..6625
                     /locus_tag="ACGI59_003112"
     CDS             5519..6625
                     /locus_tag="ACGI59_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFG9740888.1"
                     /translation="MSKKNKSLLSELKGHLMTGISYMLPLVIGSSLVVAIPKLTALAV
                     GITSLDAYKNVSGFYHILYLMEQVGWTGIGLLNTVLAGFIAFSIGEKPAMGAGFIGGL
                     IASNTNAGFLGAVIAGFFAGYVCKFLKKKIKISGSASGMMPLIIMPLITVGLTGFLMS
                     VVLAGPLGNINTSLNAWVAEMCQNGTNSVVLALILGAMIGFDLGGPVNKAAWMAGNAL
                     LLEGIYLPAILVNCAIVIPPLGYGLATFIRKSKFSNALAETGKGNIVMGIIGITEGAI
                     PFTLTNPLKLVPVNMIGCALGVGLSALLGVHAIMPPVGGLYGFISIGSGWAYLVGAIF
                     GALVIAILSTTLVDFNEDETTENTEFDEIELDIM"
     gene            6700..9378
                     /gene="mngB"
                     /locus_tag="ACGI59_003113"
     CDS             6700..9378
                     /gene="mngB"
                     /locus_tag="ACGI59_003113"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HFG9740889.1"
                     /translation="MKKTKVHVVPHSHWDREWYFTTSRSKIYLMKDFQDILDILEEKE
                     DFKYFTMDAQASLLDDYLKWRPQDKYRIRDLVQRRRLVIGPWYTQTDQLVISGESIIR
                     NLYYGIDRCNEFGEPMRVGYVPDSFGQSAQMPQIYRGFGIDSSLFWRGVSDDMVDTTE
                     FIWKGSDGSKVLAVQIPFGYYYGGNIPEDDSDLKEYLKDIIVKLKVKASTNNVYFPNG
                     FDQAPIRKNLPDILKKANEIDNENEYEISDIENYIDSVRNERNEFTELKGEFLNAKHM
                     RIHKSIFSSRSDLKIMNNKLENYITNVLEPLLTLSYSLGNEYPHLVIKDIWKLMFENA
                     AHDSIGSCNSDTTNEDVYTRYKQARDLSINLLDLHMRLISTQIKNNNEEITLTVFNGF
                     PEVRNEVVEFDTYIPGQDFVIKNKNGKILNYIIKEKEDITNYILTQTIRLNPSKKIYI
                     PKTVYRAKIALSVKDIPPMGYTQLVFELDTKNKCEIETSNCKEIENKYYKITANKNGS
                     LEILDKESNKTYKNQAVIEDNGDDGDSYNYSPPRKDIYVSSLDSVSSVEVLKSNIQEE
                     MILKYSLTIPASLEERAIGKVSVKMPVTMKITLEPKEQIIKFNVNIKNNQALSHRVRV
                     LFNTEIASKFSIADQQFGIIQRPTVLEKDLALWKRDNYSWQEKPITIEPMQSFVTLED
                     GQRGIALITGCVREYQIVGDNLDTIALTLFRSFGYMGRENLLYRPGRASGEKIIATPD
                     AQLLKELNFDFGLYIYNNKFDEANVANTAKRFLTPIQIYEYADFLNGRLIFAFNDEKQ
                     IYENEFSLMNVNNSNFTVSAIKKEEKGDGIVVRIFNGMKNEDAKGSLNINKNVNDAYS
                     AMLDEIYDNTSKLKVNTKTVEVNSLSHCKVQTIVIK"
     gene            complement(9695..12031)
                     /locus_tag="ACGI59_003114"
     CDS             complement(9695..12031)
                     /locus_tag="ACGI59_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFG9740890.1"
                     /translation="MLDTKLKKFKDKYIVTIIVFILMLSASLGMISQYSTIQASAKGG
                     AENPFEQESFVDSIYKGSYVLYHNMKEEQEGKMIQPSKLFINEETMNYIKNVSKEKDT
                     YDGSETYYTDDSINADFNENFENWEHFIKASGKNLKYYLVDKESNFEFFNDNKELGVL
                     DTTKNDASKENSDENQSKNTEMNDEERQKQITDIKENYRFYVVMNFDKDGKVNIVDSY
                     GVNQKIINQMLLSKAREDLMDTSLGGNSISYKLSPIKNITLVYAVPKVISKGDSVEYG
                     YGNTYFDDGIISYISNVENNSYYKISGMILKIILAIVVIVALAFPYRKSKELLGFDTL
                     SRIPLEILFIALAIIQGLVDLYPTVIISNTLSGNYVEYLINNDISAKMASILVSLMNL
                     TYWFVIFSIIFVFIILLKHILNVGIREYFTKHTVTGMFFVWLKKAGKTVINQIRTINL
                     KEKPNKTIVTILGINLLIIVIMCSMWFFGIILALIYSVVLFKILSDYSKKTIYEYNQL
                     LDVTKKISDGNLDVNMEKDLGFFNPIKDELGNIQTGFKKAVDEEVKSQKMKTELISNV
                     SHDLKTPLTSIITYIDLLKDESITDENRKLYIDTLDRKSQRLQHLIEDLFEVSKANSG
                     DVYLNIVNVDIVSLMKQTLLELDDKLADSSLIVKNNFSNEKIILPLDSQRTFRVFENL
                     IINISKYAMPNSRVYIDILETDNKVNIMLKNMSASEIDFSVDDIMERFVRGDKSRNTE
                     GSGLGLAIAKSFIELQGGKMNISVDGDLFKVTITFNKN"
     gene            complement(12003..12719)
                     /locus_tag="ACGI59_003115"
     CDS             complement(12003..12719)
                     /locus_tag="ACGI59_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFG9740891.1"
                     /translation="MNSYNILVVEDEKEIADAIEIYLLNQGYNVFKGYNGLEGLKVIE
                     NQEIHLAIVDIMMPKMDGITLTMKLRENYNFPVIMLSAKSEEVDKIMGLNIGADDYVT
                     KPFKPLELLARVNSQLRRYTKYLNMVGNKEQKVESDDSVFAIGGLELNENTKEVSIDG
                     KYIKTTPIEFKILSLLMRNAGRVFSADEIYERVWNENAVNTDTVMVHVRNIREKIEID
                     PKNPKYLKVVWGVGYKIEKI"
BASE COUNT         4803 a         1793 c         1672 g         4619 t
ORIGIN      
        1 tatttacttt atataaaatt atatatataa ttttatatga taataagaaa ttcttcaata
       61 tgtgtaaggc aaatatatat ttctattgca aatttttgtt atttatgata atataaatat
      121 gtttttagtc tttaaatagt tatcatttta aggaggtgtg cgtagatggc aaaagtatgt
      181 agcgtatgtg gtaaaggtaa ggtttctgga aaccaagtat cacactcaaa taaacataat
      241 aaaagaacat ggtcagctaa cttaagaagc gtaagagcga ttatagatgg agctcctaag
      301 agagtaaaag tttgtactag atgtttacgt tctggtaaaa ttgaaagagc ttagtaacca
      361 cgtttaaaag cctacttccg taagtaggct ttttaattta atcattttta ctctttctca
      421 taaattttag gattatactt gaaatccatt ttggtaattt tattgttact actttcatca
      481 atactcaccc cacactttat agttaaaact attttaacaa aaaaataata atgctccaca
      541 tccaataagt attaatgcgg taagtgctga ccaaatccag ccaggaagca acattgacag
      601 aattacagcc attcctgttg ctagacaaag tattcctagc aataccttag acttgttatt
      661 tctcatggaa cacctccaca tagaacctgt atctataaat attttcatat tcaagtttat
      721 gcgacagttt tccattttag accaataaac acattatgta attttaattt ttcatactag
      781 aattattact gaactccgat atagattcta caatatccaa aattcctttt tttctactta
      841 acctttcagg tccactatat aaaacaactg gtataacttt aggaagttta aaatttttat
      901 cttctatttc ttcaaggctt tttttcataa taaaactttt ccaaatttgt tctctatacg
      961 atttcaatct tgggacaatt tcagtatcca tttctgtttg aaattctaac actatataat
     1021 gtgatacttt gccgctttta gaaaatacct tataaattat atcaacttct tttcctttat
     1081 attcacatat aaaagatgtt tctaactcta tatttttaag agttaattct tttgctattg
     1141 agacacttgt aaaattttga atcaaactta aaaaactttc tttatctgaa tacattcttc
     1201 tgtactcttc tcgtttttca ttgcttcttt ttaaattcat acagttcctc ctagactata
     1261 gaccttctat tttttagtaa agatagtcta taaattttat tttttaattt tcactagtga
     1321 gtattgacag ttgtgtgtaa aatatccagt aaagatattt ttttatattt ttattaaaag
     1381 atataaattt ttatatttta atgtattgac atttacgttg cgtaaaagtg tatatttaat
     1441 ttataggagg tactacttat ggaatataca gttcaaaaat taagtaaact agctggtata
     1501 agtactagaa cacttagata ttatgatgaa ataggtcttc ttaagccact taaaataaat
     1561 tcttcaggtt atcgtatata tggtcaaaat gaagttaata aacttcaaca aatacttttt
     1621 tatagagaat taggaattaa cttagaaaat ataaaaaaca ttataaattc accaaacttc
     1681 gatagtttaa gtgcgttaaa agaacaccat aataaattac tagccaaaag aaagcaaatt
     1741 gatttactaa tagaaaatgt aactaaaact atagctctga aggagggaaa atacactatg
     1801 acagatatag aaaaatttga aggttttaaa gaaaagatga ttgatgaaaa tgaaaaaaat
     1861 tatggaactg aaattagaga gaaatatggt aaagatatta tagatacatc aaataaaaaa
     1921 cttaaaaata tgtctaagca agattatgaa aattggcaaa acctaaatat cgaaataatt
     1981 agtaaactta aaaaagcttt taaaactggt gatgcctcta gtgaactggc tcaggaagtt
     2041 gcaaaattac atcataaatg gttgtgttac acttggaaca catactctaa agaagctcat
     2101 tcctctctag cacagatgta tgtatatgat gaacgtttta catcatatta tgatagagaa
     2161 caacctggat tagctaagtt tttaagggat gctatagtaa tatatacaag agataaataa
     2221 tataaaataa taaaggtggt gtctcaaaat gaacttttta gttcatgaga cactcttttt
     2281 tgtatgaaat cagatatatt tttatcattt tatcaatgaa aaacaggttt ctctctctat
     2341 tttgagacag aatctttatt gtataataat tataatcaat acaaaataat aacaaattat
     2401 acaaaactta cttatactat ttatgctaaa aacaaaaaat attttattaa agtttttaaa
     2461 acttatgtaa tataaaaatc tatactatag acttgattat ggaaagaaaa ttatcaaaat
     2521 cattacaatt agatatacta tctctaatat cattgtcatg aattatcttt aaaaggatat
     2581 cataaatgga gtttattttt atataatcat ctttatttat agccaacatc atgattaatt
     2641 taacagtaga attttcactc caatatattg gttcattcaa tatggaaata caaatcttag
     2701 ttcttataga gcttaattcc ataggatgag gtatagctaa aaaatcatct atattggtag
     2761 aagataaact ttctctgtaa aatacagatt cagcaaatga tggaaataca atttcacttt
     2821 gagttaattt gttgcacatc aactttataa gctcatcttt atctttgatt ttaggattaa
     2881 caataaataa atttttatca aagaaattgt ccaaaagatt actataaata tgggaattat
     2941 tagtgattga cttagatata ttctctatat ctttttttaa tagttttaaa tccactaata
     3001 caataggaat actatcatga tttaaaggaa ttgtcgatat tattatatct acatctgaca
     3061 attcactagc taaaaattga ttaaatgata gacattgaag tatatttatt ttatcatgaa
     3121 aaactttgtt taattgaacc tctaataacc tacttgaacc ataccctgaa ccacatacta
     3181 atactacgtt tttacactta atattacttt gaaaaaatct ctctattcca gcacctatat
     3241 gcagggatac atatccaatt tcatcttctg ttaaagagta aatactattt ttaaaaactt
     3301 tttctatagc atttaaagtt atttcaaatg ctaaaggata attactcttt attgtattta
     3361 taaggggatt caccttattt aaattataag atttagaatt gagaattgat ttaaagtgca
     3421 gtactaaatc atcaaataat acactatcat ttctaaggtc aaaagtatat tgattgttga
     3481 ttacttctaa aatttcttca acatactcta taattttagt atctacccta ttggaaacgt
     3541 tatccaaaag agaactttta gttataaaat gattatatag ataaactctg tctgcctttg
     3601 aaataattat agtatattta ctctcaatat aattaaatat attttcaaca gttgtattcg
     3661 attgaaaatc tgttaaaaca acatctaaaa cattatctaa gcagtaacca tcaagtattc
     3721 tacttacagt aatagctaaa tggataataa agttttttag attaaaatct aggaaattga
     3781 tattaaattt attaatttca cttaacacta ccctttgaag ctctattaaa tctacattat
     3841 taaataattc tatttctcta tctgtaattc caattatata gttttcaaaa ttcctattta
     3901 tcatattatt agaaatacat cttcgaatat ctatttcctt accttcaact ttaactccat
     3961 aatatggctt agaaactaat attaaattat actttgacaa aataggcttt attcttttta
     4021 tgtcatttag tattgtaaat ttactcacat acaagctatt agctaaatct tctagtttta
     4081 tatatgtatt gttatagaga atatatttta aaatatattg atttctttct ataggagaat
     4141 ctggtatttc acttgaatcc ataatatcat cagatattaa tgctagatac tgttggtata
     4201 atgctaaatt aagaatatct atataatatc cttctccttt ttttatttta ataatagcac
     4261 catttttctc taaagttcca tttatagaag taatatcatt tcttatagtt ctctcactca
     4321 cttttaaatg attacttaaa gttttacaag taattttatc atcactttca tttaataatt
     4381 taaacaacgt gagcaatctc ttgtgcataa atatcccccc ttatagaatt tcttctaaat
     4441 tatactattt acactcatat aatacaatta ctctaataaa attgtattat aataatttat
     4501 ttccacttct atatggaaat tagttattat cataatataa atttccatgt cgaagtggaa
     4561 attagttata tttaatttaa ttaggataat gattacaata taaatataag ttaagacatt
     4621 atgaatggag tggaaaaata tatggtacta caagaaaaat atatatttat agataaagac
     4681 ttaaaaagca aagaagaagt attaaatttt attgcagata gtgcttatat actaggtatt
     4741 acaactaata aagaggctct tttaaaagac cttctaaaaa gagaaaatga atttccaaca
     4801 ggaataaaag atggatttgc gatacctcat acaaagagtg taaatgtaaa ggaagcttcc
     4861 atactctata ttaaaacaac taacgagtta gaatggaaaa ctttagatga tagtaaggtt
     4921 agatatgtat ttagtttgtt agccccacat aaaaatgaag gaaatataca tttgaaaact
     4981 ctatctaaat tagccacatg tttaatggag gatgatttta caagtgaagt taaaaatgct
     5041 agtgatgcaa gtcatttagt aaaatatata actaataaaa tgaaggagga ataattatga
     5101 aaatagtagc tataacatca tgtatagcag gattagcaca cacacctatg gcagcaaaag
     5161 ctttagaaca agctgcatca aaaatgggtc atgaaatcaa agtagaacaa caaggtgcta
     5221 tgggacgagt aaatactata acaaaagaag aagcacaaga tgcggacttt atacttatag
     5281 catctgacca aactatatct ggaatggaaa gatttgatgg taaaaaagtg ataaaagtca
     5341 aaataggtat agcgttaaaa aaaccagaag ctgtcttaac aaagtgtata gaagctattt
     5401 cttaataaaa aaatctcact ctaatataga atgagaaaaa tcaaaaatta aaaaattgtc
     5461 taatgtaatt ataacatatt atcaaaaaat ataacaaact tatataggag gattaaaaat
     5521 gagtaaaaaa aataaatctc ttttaagtga acttaaggga cacttaatga caggtatctc
     5581 atatatgttg ccacttgtaa taggctcatc tttagttgta gctatcccaa agcttacagc
     5641 tttagcagtg ggtataacaa gtttagatgc ttataaaaat gtatctggtt tttatcatat
     5701 tctttactta atggaacaag ttggttggac tggcataggt ctattaaata cggtattagc
     5761 aggatttata gcattttcaa taggtgagaa accagctatg ggtgcaggtt ttattggtgg
     5821 gctcatagca agtaatacta atgcaggatt cttaggagca gtaatagcag gtttctttgc
     5881 tgggtatgtt tgtaagtttt taaagaaaaa aattaaaata tctgggtcag catcaggaat
     5941 gatgccactt ataataatgc cacttataac agttggatta actggttttt taatgagtgt
     6001 tgtattagca ggacctttag gaaacataaa tacttcatta aatgcttggg tagctgaaat
     6061 gtgtcaaaat ggaacaaatt cggtggtatt ggctttaatt ttaggcgcaa tgataggatt
     6121 cgatttaggt ggaccagtaa ataaagcagc ttggatggca ggaaatgcat tattgttaga
     6181 gggaatctat ctaccagcga tattggtaaa ctgcgcaata gttatacctc cactaggata
     6241 tggtttagct acattcataa gaaaatcaaa attttccaat gcattagctg aaacaggaaa
     6301 aggcaatata gttatgggta taattggaat aacagaaggt gcaattcctt tcactttaac
     6361 aaatccatta aaattagttc ctgttaatat gataggatgc gctttgggtg taggattatc
     6421 agctttgtta ggtgtacatg ctataatgcc tccagttggt ggattatatg gatttatatc
     6481 aataggttct ggatgggctt acttagtagg tgcaatattt ggagctttag taatagcaat
     6541 attatcaaca actttagtag acttcaatga agatgaaact acggaaaata cagaatttga
     6601 tgaaatagag ttagatataa tgtaaattaa aaacttaatt ttagataatt gagaattgag
     6661 tgtactctca attctcaatt aaattttggg aggattttta tgaaaaagac aaaagtacat
     6721 gtagttccac acagtcattg ggatagagaa tggtatttta caacatcaag gtcaaaaata
     6781 tatttaatga aagattttca agatatatta gacatattgg aagaaaaaga agactttaaa
     6841 tactttacta tggatgcaca agcttctctc ttagatgatt atttaaaatg gagacctcag
     6901 gataaatata gaataagaga tttagttcaa agaagaagat tagttattgg accttggtat
     6961 actcaaacag accaattggt tatatcagga gaatctataa ttagaaacct ttactatgga
     7021 atagatagat gcaatgaatt tggtgaacct atgagggttg gatatgtacc agattctttt
     7081 gggcaatcag ctcaaatgcc acaaatatac agaggttttg gaatagatag ttcattattt
     7141 tggcgtggag tttctgatga tatggtagat acaacagagt ttatatggaa aggttctgat
     7201 ggttcaaaag tattagcagt tcaaatacca tttggttatt actatggagg taatatacca
     7261 gaagatgatt cagatttaaa agaatattta aaagatataa tcgtaaaact taaggttaaa
     7321 gcttctacta ataacgtata ttttccaaat ggatttgacc aagcaccaat aagaaaaaac
     7381 cttcctgata tattaaaaaa agctaatgaa atagataatg aaaatgaata cgaaataagt
     7441 gatatagaaa actatataga ttcagtaaga aatgaaagaa atgaatttac agagcttaaa
     7501 ggtgaatttt taaatgctaa acatatgaga atacacaaat ccatattctc ctcaaggtca
     7561 gaccttaaaa taatgaataa caaattagaa aattatatta caaatgtact tgaaccgtta
     7621 ttaacattaa gttattcact tggaaatgaa taccctcacc tagttataaa agatatatgg
     7681 aaacttatgt ttgaaaatgc agctcatgac agcataggtt cttgtaatag tgacacaaca
     7741 aatgaggatg tatacacaag atacaaacaa gccagagatt tgagtataaa tttattagat
     7801 ttgcatatga gacttataag tactcaaatc aaaaataata atgaagaaat aacattaaca
     7861 gtattcaatg gattcccaga agtcagaaat gaagttgtag aatttgatac ttatattcca
     7921 ggtcaagatt ttgtgataaa gaataaaaat ggaaaaattc ttaattatat aataaaagaa
     7981 aaagaagata ttacaaatta tatattaact caaacaataa gattaaaccc aagtaaaaaa
     8041 atatatatac ccaaaactgt atatagagca aaaattgcac tttcagtaaa agatatacca
     8101 ccaatgggat atactcaact agtttttgaa ttggatacta aaaataaatg tgaaatagaa
     8161 acaagtaatt gcaaggaaat agaaaacaaa tactacaaaa taacagccaa taaaaatggt
     8221 tcattagaaa tattagataa agaatctaat aagacttata aaaaccaagc tgtaattgaa
     8281 gacaatggag atgatggaga ttcatacaac tattctcctc caagaaaaga tatttatgtt
     8341 tcatctttag attctgtatc ttcagtagaa gtgctaaaat caaacattca agaagaaatg
     8401 atacttaaat attcattgac cataccagct tcactggaag aaagggctat aggtaaagtt
     8461 tctgtgaaaa tgccagtaac aatgaaaatc accttagaac ctaaagaaca aataattaag
     8521 tttaatgtta atataaaaaa taaccaagca ctatctcata gagtaagagt tttatttaat
     8581 acagaaatag catctaagtt ttctatagca gatcaacaat ttggaataat acaaagacca
     8641 acagtattag aaaaagacct tgcattgtgg aaaagagaca attactcatg gcaagaaaaa
     8701 cctataacta tcgaaccaat gcaaagtttt gttactttag aggatggaca aagaggaata
     8761 gcattaataa ctggttgtgt aagagaatat caaatagttg gtgataattt agatacaata
     8821 gcattaacac tattcaggtc atttggatat atgggaagag aaaatttact ttatagacca
     8881 ggaagagctt ctggagaaaa aataatagca acaccagatg cacaattact taaagagtta
     8941 aactttgact ttggtcttta catatataac aataaatttg atgaagcaaa tgtagctaat
     9001 acagctaaga gattcttaac acctatacaa atatatgaat atgctgattt tttaaatggt
     9061 agacttatat ttgcattcaa tgacgaaaaa caaatatatg aaaatgaatt tagtttgatg
     9121 aatgtaaata attcaaattt cacagtaagt gcaataaaga aagaagaaaa aggagatgga
     9181 attgtagtta gaatcttcaa tggtatgaaa aatgaggatg ctaagggaag tcttaacatc
     9241 aataaaaatg tcaatgatgc ttatagtgca atgctcgacg aaatctatga taatactagc
     9301 aagcttaaag taaatactaa aactgtagag gtaaattctt tatcacattg taaagtacaa
     9361 acaatagtaa ttaaataata taaaatagct tatacaagat ttcaaaataa attgctagta
     9421 ccatgttaat agtaaagcca gatattatgc tatttataag ctctgtaaca ctaaataaca
     9481 ttttgtaaaa taaaaaggtg aaccttgtat attaggattc acctttttta aataaatcaa
     9541 cattctgttt taaaataaca tttataagct gttttgaaat aatgcttaca aattatttta
     9601 aaacaatacc tataaatttt tatttaaaga atacacataa atatttccag actcttttat
     9661 cctatagact ttataattat taaagaagtc taatctaatt tttgttaaaa gtaattgtaa
     9721 ctttaaataa atcaccatcc acacttatat tcatcttacc accctgtaat tctataaaac
     9781 tttttgctat cgctaaacca agaccagaac cttctgtgtt acgtgattta tctcctctta
     9841 caaatctctc cattatatca tctacactaa aatcaatctc agatgcagac atatttttaa
     9901 gcataatatt tactttatta tctgtttcta aaatatcaat atacactctt gaatttggca
     9961 ttgcatattt gcttatattt attataagat tttcaaatac tctaaatgta cgctgactat
    10021 ctaatggtaa tattattttt tcattagaaa agttattctt tactattaga gatgaatctg
    10081 caagtttgtc atctaattct aaaagtgtct gcttcattag agatacaata tccacattta
    10141 ctatattcaa ataaacatct ccactatttg ccttgcttac ttcaaacaaa tcttctatta
    10201 gatgttgcaa tctttgtgac tttctgtcta atgtatcaat atacaactta cgattttcat
    10261 ctgttatact ttcatctttt aataaatcta tataagtgat tatagatgtt agaggtgttt
    10321 ttaagtcatg tgatacattt gatataagct ctgttttcat cttttgactc ttaacctctt
    10381 cgtcaacagc ttttttaaat ccagtttgaa tatttcctaa ttcgtctttt ataggattaa
    10441 aaaatcctaa atctttttcc atatttacat ctaagtttcc atctgaaatt ttctttgtaa
    10501 catctaaaag ttgattatat tcatatattg tctttttaga gtaatctgac agtattttaa
    10561 ataatactac tgaatatatt aatgcgagta ttattccaaa gaaccacata ctacacatta
    10621 taactataat caataaattt attcccaaaa ttgtaactat agttttattt ggcttttctt
    10681 ttagatttat tgttctaatt tgattaataa ctgtttttcc tgcttttttt agccatacaa
    10741 aaaacattcc tgtcacagta tgttttgtaa aatactctct tattccaaca tttaaaatat
    10801 gttttaaaag tattataaat acaaatatta ttgagaatat tacaaaccaa taagttaaat
    10861 tcattaaact taccaatata ctagccattt tagcacttat gtcattgttt ataaggtact
    10921 ctacataatt cccactcaag gtattactta ttataacagt aggatataaa tctacaagcc
    10981 cctgtataat agcaagagca ataaagagta tctctaacgg tattcttgat aatgtatcaa
    11041 atcctagcaa ctctttagat tttctatatg ggaatgctaa agctacaata acaactatag
    11101 ctaatataat ttttaaaatc attccagaaa tcttataata agaattattt tctacattgc
    11161 ttatatatga aattatacca tcatcaaaat aagtatttcc ataaccatac tctacactat
    11221 cacctttact tattacttta ggtacagcat atactaatgt tatatttttt attggagata
    11281 acttatatga aattgaattt cctcctaaac ttgtatccat taaatcttct cttgctttag
    11341 ataacaacat ttgattaata attttttgat ttactccata agaatccaca atatttactt
    11401 ttccatcttt atcaaaattc attacaacgt agaatctata gttttctttt atatctgtaa
    11461 tctgcttttg tctttcctca tcattcattt ctgtattttt tgactgattt tcatcagaat
    11521 tttcttttga agcatcattt tttgttgtat caagtactcc taattcttta ttatcattaa
    11581 aaaattcaaa attgctttct ttatcaacta aatagtactt taaattcttt cctgacgctt
    11641 taatgaaatg ctcccaattt tcaaagtttt cattaaaatc tgcattaata ctatcatctg
    11701 tatagtaagt ttctgaacca tcataagtat ctttttcttt acttacattt tttatatagt
    11761 tcatagtttc ttcattgata aatagttttg atggttgtat catcttacct tcttgttctt
    11821 ccttcatatt atgatataaa acataacttc ctttatatat actatctaca aagctttcct
    11881 gttcaaatgg attctctgct ccacctttag cacttgcttg tatagtagaa tattgactaa
    11941 tcatacctaa tgatgctgat aacataagta taaaaactat aatagtaact atatatttat
    12001 ctttaaattt tttcaatttt gtatccaaca ccccacacca cctttaaata tttaggattt
    12061 tttgggtcaa tttctatctt ttcccttata ttcctcacat gtaccataac tgtatctgta
    12121 tttacagcat tctcattcca aactctttca tatatttcat cagcagaaaa aactcttcct
    12181 gcatttctca taagtaaact tagtatttta aattctatag gagttgtttt tatatacttt
    12241 ccatcaatac taacttcttt tgtattttca tttaattcta atccgcctat tgcaaacaca
    12301 ctatcgtcac tttctacctt ttgctcttta tttccaacca tgtttagata cttagtgtac
    12361 cttctaagtt gtgaattaac tctagccaat agttctaatg gcttaaaagg ttttgttaca
    12421 taatcgtctg ctcctatatt aagccccatt attttgtcaa cttcctctga ttttgcagat
    12481 agcattatta ctggaaaatt atagttttct ctaagcttca ttgtaagtgt tataccatcc
    12541 attttaggca tcattatatc aactatagct aaatgtattt cttggttttc tataacctta
    12601 agaccttcta atccattgta tcccttaaaa acattatatc cttgatttaa tagatatatt
    12661 tctatagcat ctgctatctc cttttcatct tctaccacca aaatattata tgaattcatt
    12721 ctaatctcct ttatattaat ttataaattt tctttacata tatgattata caatatgata
    12781 gaagtttttc catttatata tatatttata tcttttgttc tggttatttg tatttaatgt
    12841 ccatcttaga aacctccttc tagtttaaat ttaatatttt ttcattt
//
LOCUS       DAYAPA010000123        12495 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.125, whole genome shotgun sequence.
ACCESSION   DAYAPA010000123 DAYAPA010000000
VERSION     DAYAPA010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12495
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.125"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            <1..557
                     /locus_tag="ACGI59_003116"
     CDS             <1..557
                     /locus_tag="ACGI59_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9740892.1"
                     /translation="LFNGEISPIVLALAFVFICKVLLDLFLKTGLGYTLKGVGDNSQM
                     IKSLGINIGSIKILGLMISNGLIALSGGLMAQFLGFSDVNMGIGTLVLGIASIIIGIT
                     LFKKFTFIKDTTAIIVGSFIYQFTIYFAMSLGMLSTDLKLITAIVIIAFLATGNLNIS
                     LKKNKCKASTKNKSEKRGVVDVTN"
     gene            544..1299
                     /locus_tag="ACGI59_003117"
     CDS             544..1299
                     /locus_tag="ACGI59_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9740893.1"
                     /translation="MLQIKNLSKSFPNPYGEPNTIFENLSIDIEDGEFVSIIGSNGTG
                     KSTLLNIISGLIKESSGQITLDSTTITNLAEYKRTQIVSRVFQDPSLGTCPSMTVREN
                     LSLALNKGKLLNLKKCLRHKTNDLEHLLEGISLDLKKYLDIKVQYLSGGQRQSLSLIM
                     SSLASPKVLLLDEHTAALDPKTSNEVIELTDKIVREKNITTLMVTHNLKHALQYGDRL
                     IMLHKGEVVLDVKDKEKKDLTVEEILEKFEYAV"
     gene            1396..2433
                     /locus_tag="ACGI59_003118"
     CDS             1396..2433
                     /locus_tag="ACGI59_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFG9740894.1"
                     /translation="MIKSKKILSLIIAGVLGVSMLTGCSQNDGSNASNENKETDSKKQ
                     KNIGITQLVEHPSLDKAKKGFIKALEDKGYKDGDNIKIDFQNAQNDMPTTQSIASKFV
                     SDKKDLIYAISTPSAQAAYNATKDIPIIMTAVTDPVEAGLVKSLEKPGGNVSGTSDYL
                     SIDKTLELVKTLTPKAKKIGVIYNTSEVNSKIQVDSLHDYAKKNNYEVVEKGISSSSE
                     VNQAISSLVGKIDVLYVPTDNLIVSSMPIVSKVANENKIPIIASEEGSVSSGALACCG
                     IDYEKLGYKAGELAIEVLEGKSVGDIPVTTLDETEIIINEDTLKALDMQKLSADNIKY
                     IKSDENAKSTK"
     gene            3242..4144
                     /locus_tag="ACGI59_003119"
     CDS             3242..4144
                     /locus_tag="ACGI59_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9740895.1"
                     /translation="MSGIISVMTQSLILSIMALGVYITYKILDFPDMSADGSYTMGAS
                     IVAFSLTNGISPVVATLMAILCGCTAGLVTGILHIKFKISNLLSGILVMGMLYSINLR
                     IMGKSNIPLFSFKHLFNGEISPIVLALAFVFICKVLLDLFLKTGLGYTLKGVGDNSQM
                     IKSLGINIGSIKILGLMISNGLIALSGSLMAQFLGFSDVNMGIGTLVLGIASIIIGIT
                     LFKKFTFIKDTTAIIVGSFIYQFTIYFAMSLGMLSTDLKLITAIVIISFLATGNLNIS
                     LKKNKCKASTKNKSEKRGVVDVTN"
     gene            4131..4886
                     /locus_tag="ACGI59_003120"
     CDS             4131..4886
                     /locus_tag="ACGI59_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9740896.1"
                     /translation="MLQIKNLSKSFPNPYGEPNTIFENLSIDIEDGEFVSIIGSNGTG
                     KSTLLNIISGLIKESSGQVLLNKHNLSNLAEHRRTQIVSRVFQDPSLGTCPSMTVREN
                     LSLALNKGKLLNLRKCLRYKRDFLENLLEGVSLDLKKYLDVQVKFLSGGQRQSLSLIM
                     SCLTNPSVLLLDEHTAALDPKTSNEVIELTNKIVREKNITTLMVTHNLKHALHYGDRL
                     IMLHKGEVVLDVKGKEKEQLTVEEILEKFEYAV"
     gene            5017..6039
                     /locus_tag="ACGI59_003121"
     CDS             5017..6039
                     /locus_tag="ACGI59_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD0873 family tyrosine ABC transporter
                     substrate-binding lipoprotein"
                     /protein_id="HFG9740897.1"
                     /translation="MINKKRLASLILAGALSISMLTGCSQGGDSGNSKQESNSKGKEV
                     KKIGITQLVEHPALDATRTGFVKALEKNGFKDGENIDIDFQNAQNDMPTTQSIASKFA
                     SDKKDLIFAISTPSAQAAFNATKDIPILITAVSDPVAAGLVKTLEKPGTNVSGTSDFV
                     SVDKGLELLKIFAPKAKTIGVMYNTSEVNSKVQVDALKEYASKNGFKVVEKGITTSNE
                     VNQGISSLVGKIDVLYVPTDNLVASSMPIVSKIATENKIPVIAAESGPVEKGALACQG
                     INYEKLGYKTGEMAVKILNGESVSDMPVATSDDTDIIVNEDILKALGMEKPSNENISY
                     VKTKQE"
     gene            complement(6203..6760)
                     /locus_tag="ACGI59_003122"
     CDS             complement(6203..6760)
                     /locus_tag="ACGI59_003122"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HFG9740898.1"
                     /translation="MTEKEKMLSGKGYYANDELLVKEREYCKKLTRLFNNTLEDEYEK
                     REDILRQLFGSVGKQINVEQNIRCDYGYNIHVGENFFANYDCIFLDVCKIEIGDNVML
                     APNVQIYTAYHPIDAQLRNSGIEYGSPIKIGDNVWIGGGVIITPGITIGDNVVIGAGS
                     VVTKDIPPNTVAVGNPCRVIKKIEE"
     gene            complement(6766..7842)
                     /locus_tag="ACGI59_003123"
     CDS             complement(6766..7842)
                     /locus_tag="ACGI59_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HFG9740899.1"
                     /translation="MSLYVDIEKDLSSFKLKVEIKQEKGTLGFLGESGSGKSMTLKCI
                     AGLEKPTRGKIVLNDRVLFDSEKKINLSTQDRKVGFLFQNYALFPHMTVSQNIELGLL
                     KLSKSEKKEIVARYLDILKLNGFEGRYPWQLSGGQQQRVALARALATSPDILLLDEPF
                     SALDHHLRSNMEKELMNMLKDYKGDILFVTHDIEEAYRVCDDIIVYNKGEGLPKRPKK
                     ELFESPKYLIEAKITGCKNISKLNRLDKNTIYATDWGCELTLNREIGDNIEYVGIREH
                     HIKVLDSNEDLNEKLCFELINIVENPFTYTIYVRKTDLSNECVPIQIELEKSKMRFKK
                     GDRIYLDFPQEYLFCFRYNYNKKE"
     gene            complement(7843..8526)
                     /gene="modB"
                     /locus_tag="ACGI59_003124"
     CDS             complement(7843..8526)
                     /gene="modB"
                     /locus_tag="ACGI59_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HFG9740900.1"
                     /translation="MGTDWSPLWISLKTSILSTIITFVIGIFVSYIMANYRGKWKGLI
                     DGLFTLPLILPPTVVGFFLLLLCGKNGFIGKFLLNFDRTLIFSWTATVISAVVVSFPM
                     MYRTSRSAFEQIDNNMLSAARTLGLNEWKVFFKIAVPLAWPGIIGGLVLSFARALGEF
                     GATLMIAGNIPGKTQTMPIAIFFAVEGGDMNKAMMWVMIIVAISIAMILLLNYWSEFQ
                     QKIIGKRCG"
     gene            complement(8675..9487)
                     /gene="modA"
                     /locus_tag="ACGI59_003125"
     CDS             complement(8675..9487)
                     /gene="modA"
                     /locus_tag="ACGI59_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFG9740901.1"
                     /translation="MKKILGILGLVACLTLGTVGCNSNEDKKDDGKQEKTTKSSDSVE
                     LNISAAASLKEAMAKIEEEYKKVDSNVKLTVNYGASGSLQQQIEQGAPCDLFISAGQK
                     QMKALDEEKLLVSDTMKDLVKNDLVLISSADSSVSGMKDLTTDKVKKIAVGEAESVPA
                     GKYADEVLTNLNLKDKLKDKLVFAKDVKEVLAWVQSGNADVGFVYFSDTVNNDKIKVV
                     EKTDEKTHSPITYPVSVIKASKNVDAAKKFEEFLLSESGQKIFEEFGYKKVE"
     gene            9680..10609
                     /locus_tag="ACGI59_003126"
     CDS             9680..10609
                     /locus_tag="ACGI59_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFG9740902.1"
                     /translation="MDSLSSLTPVEVAELLKITKNTVYELIKRGELPSYKVGKKIRVD
                     TKDVEEYINSQKTGKVKDTHFNNKNIDINNNFKIIISGQDVILDILARSVEKKLDGIS
                     TFRSYIGSYNALYELYNGRVSIASSHLWDFETDEYNSTFVKKLLPGIPCVLINLAYRM
                     QGFYVAKGNPKEITTWEDLTKPSITIVNREKGSGTRVLLDGKLRLLNFNGKHIKGYDN
                     EELSHLGVASTVSRGIADVGLGNEKAALQVNNIDFIPLHKERYDLVIKKEDLQNPVYQ
                     TIINIINSPEFKAELQGLGGYDLTDTGKTIAKV"
     gene            complement(10770..11219)
                     /locus_tag="ACGI59_003127"
     CDS             complement(10770..11219)
                     /locus_tag="ACGI59_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9740903.1"
                     /translation="MKNKNNKDKNMLNIKVLRVLGVLLILFDYTGDTLNFYNFFAKPL
                     VSGNLYKTTGTDCIVFLLFIVECVYLVGRYILKVKINWSEIIENEKGNFINNLLVEKI
                     QEESNIVYNLLNSYVLPNIYKKARGLMFKTYHMLIYIKEKYYYVNIY"
     gene            complement(11422..12402)
                     /locus_tag="ACGI59_003128"
     CDS             complement(11422..12402)
                     /locus_tag="ACGI59_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFG9740904.1"
                     /translation="MKNSFKNCISLRNKFIIISIVFLLILLIVSYIFGKLVYNSSVGL
                     EQLIKNEDIVKIFSERKDRPLEKLKNYKTSELMIQSSNNYKLESLFITSNIKTRDTMI
                     LVHGIGSSYYEMLKVAYRYLDKGYNVLVYNQRNTGNSGGDNYTFGLYERYDLDSLVKF
                     VKNKFPEGRLGVHGFSMGAGTAAMHSEINSKDDKVDFYILDSPYSEMKDAIRMGVLEK
                     RIPDILINYVVTCGDLYNKFKSGFWYSDVKPYESVEKSNVPILFIHGTKDTVCNYQNS
                     KKMYDLVKHDKKDLWLIEGIGHVDGFEHDSTVYFNKIFKFIDSNVLSDKS"
BASE COUNT         4421 a         1927 c         1630 g         4517 t
ORIGIN      
        1 acttattcaa tggggaaata tctcctatag ttttagcctt agcttttgta ttcatctgta
       61 aagtcctttt agatttattc ctaaaaacag gtcttggata cactttaaaa ggtgttggtg
      121 ataactctca aatgattaaa tctctaggta taaatatagg ttctataaaa atactaggac
      181 ttatgatttc aaatggactt atagctttat ctggtggtct tatggctcag ttcttgggat
      241 tctcagatgt aaatatgggt ataggaactt tagttcttgg aattgcctct atcataattg
      301 gtattacact atttaagaaa tttactttta tcaaagatac tactgcaata attgttggct
      361 cttttatata ccaattcact atatactttg caatgagttt aggaatgtta tcaactgact
      421 taaaactaat aactgccata gttataatag ctttcctagc tactggtaac ttaaatattt
      481 cactaaaaaa aaacaaatgc aaagctagta ccaaaaataa atcagaaaaa agaggtgttg
      541 tagatgttac aaattaaaaa tctttcaaag agtttcccta atccatatgg tgaacctaat
      601 actatctttg aaaatttatc aatcgatata gaagatggtg aatttgtaag tatcataggt
      661 agtaatggaa ctggtaaatc tactttatta aatataatat ctgggcttat aaaagaatct
      721 tctggtcaaa ttactttaga tagtactact ataacaaatt tagctgagta taaaagaact
      781 caaatagtaa gtagagtttt tcaagaccca agccttggaa cttgtccttc tatgacggtt
      841 agagaaaatt tatctctagc acttaataaa gggaaattat tgaacttaaa aaaatgtctt
      901 cgtcataaaa cgaatgactt ggaacactta cttgaaggaa tttctttgga tttaaaaaaa
      961 tatttagata ttaaagtaca atatctttca gggggtcaaa gacaatcact atcactaatt
     1021 atgtctagct tagcaagtcc taaggtctta ttactagacg aacatacagc agcacttgac
     1081 ccaaaaacat caaatgaagt aatagagtta acagataaaa tcgtaagaga gaaaaatata
     1141 actacactta tggttactca caacttgaaa cacgctcttc aatatggaga taggttgata
     1201 atgcttcata agggtgaagt tgtattagat gtaaaagata aagaaaaaaa agatttaaca
     1261 gttgaagaaa tcttagaaaa atttgaatat gcagtataag aaattaaaat cttagtttta
     1321 atcagaaata tttataatct atcatatgaa tatatgaaca aatataatta ttaaatttta
     1381 agggaggaaa ataaaatgat aaaaagtaaa aaaatattaa gtttaattat agcaggagta
     1441 cttggtgtat caatgcttac tggatgttct caaaatgatg gctccaatgc ttcaaatgaa
     1501 aataaagaaa cagatagcaa aaaacaaaaa aatataggta ttactcaatt agtggaacat
     1561 ccatctctgg ataaagcaaa gaaaggattc atcaaagcac ttgaagataa aggctataaa
     1621 gatggagata atataaaaat agatttccaa aatgcacaaa atgatatgcc tactacacaa
     1681 agtattgcta gtaagtttgt atccgacaaa aaagatttga tatatgctat atctactcca
     1741 tctgcacaag cagcttataa tgctactaaa gatattccaa taataatgac tgctgttaca
     1801 gaccctgtag aagctggact tgttaaatct cttgaaaaac caggtggaaa tgtttctggt
     1861 acatctgatt atctttcaat tgataaaaca ttagaattgg ttaaaacatt gactccaaaa
     1921 gcaaagaaaa taggggttat atacaatact agtgaagtta attcaaaaat ccaagttgat
     1981 tctctacatg attatgctaa gaaaaataat tatgaagtag ttgaaaaagg aatcagctct
     2041 tcaagtgaag ttaaccaagc tatttctagt ttagttggta aaatagatgt tttatatgtc
     2101 cctactgaca atttaatagt ttcttctatg ccaatagttt ctaaagttgc taatgaaaac
     2161 aagattccta taatagcctc tgaagaaggt tctgtatctt ctggtgcttt agcttgttgt
     2221 ggaatagatt atgaaaaact aggttataag gctggagaac ttgctattga agtattagaa
     2281 ggaaaatctg ttggcgatat accagttact acattagatg aaactgaaat aataattaac
     2341 gaagatacac taaaagcact tgacatgcaa aagttatcag cagataatat aaagtatata
     2401 aagtcagatg aaaatgcaaa atctacaaaa taaaacttat taatttcaaa aaataggtaa
     2461 tatgaagtag gtaatatgaa aactttatat aataagtact cttatccacc ctaacccttt
     2521 gaaaattgtg cttattgtag caatttgaat attgcatata aagttattca ttttccataa
     2581 aagtcgtcta tattgacgac tgattatgaa agttatataa ctttctaatc actacttgag
     2641 ctgtccaaaa gaattttaat attctccaga cagctttttt ttatgatatt tgattgatat
     2701 acatgtctag ttattccata tttagtaaat attttgataa aatttccaca ttcacttata
     2761 acttaggata acgttttttt caaacaatac attaatttaa aagtattctg agtatacatt
     2821 atttagtatt ttaattaaat ttagcagttg acttttatta ttgtataatt tataatttat
     2881 aacaagtcga gaatacatat aattttctga ctaattaaaa tattaaaagg gagagataat
     2941 tatgaatact ttattaaaca atagaaaata ttattattat tatagccact atttttaggg
     3001 tttgtaccta cgttttaaca tagatacaga ccccttaaga ctactaaaat tttgggattg
     3061 tatctatgtt ggctatattg tttataaagt tttcatctaa ctatataaga gccacataga
     3121 ttaaactatg tggcttattc gtgtttttac cacatagttt tgtacactat gtggtttttt
     3181 tatacacaaa attagcaaat aattaaaatt ataaaaatat tttcaaagga gtgttttatt
     3241 tatgtcaggt attatatctg taatgacgca aagtttaatt ttatcgatta tggcactggg
     3301 ggtttatata acttacaaaa tcttagattt tccagatatg tctgctgatg gtagctacac
     3361 aatgggtgct tctattgtag cattttctct tactaatggt atttctcctg tagtagcaac
     3421 tttaatggct atcttatgtg gttgtactgc tggtcttgtt acagggattc ttcacattaa
     3481 gtttaagata tctaacttac tttctggtat tttagttatg ggaatgctat actctatcaa
     3541 tttaagaata atgggaaaat caaatattcc tctatttagc tttaaacact tattcaatgg
     3601 ggaaatatct cctatagttt tagccttagc ttttgtattc atctgtaaag tccttttaga
     3661 tttattccta aaaacaggtc ttggatacac tttaaaaggt gttggtgata actctcaaat
     3721 gattaaatcc ctaggtataa atatcggttc tataaaaata ctaggactta tgatttcaaa
     3781 cggacttata gctttatctg gtagtcttat ggctcagttc ttgggattct cagatgtaaa
     3841 tatgggtata ggaactttag ttcttggaat tgcctctatc ataattggta ttacactatt
     3901 taagaaattt acttttatca aagatactac tgcaataatt gttggctctt ttatatacca
     3961 attcactata tactttgcaa tgagtttagg aatgttatca actgacttaa aactaataac
     4021 tgccatagtt ataatatctt tcctagctac tggtaactta aatatttcac taaaaaaaaa
     4081 caaatgcaaa gctagtacca aaaataaatc agaaaaaaga ggtgttgtag atgttacaaa
     4141 ttaaaaatct ttcaaagagt ttccctaatc catatggtga acctaatact atctttgaaa
     4201 atttatcaat cgatatagaa gatggtgaat ttgtaagtat cataggtagt aatggaactg
     4261 gtaaatctac tttattaaat ataatatccg ggcttataaa agaatcttct ggtcaagtat
     4321 tattaaataa acataacttg tctaatttag ctgaacacag aagaactcaa atagtaagta
     4381 gagtttttca agacccaagt cttggaactt gcccttctat gactgttaga gaaaacttgt
     4441 ctttagctct taacaaagga aaactattaa atctaagaaa atgtcttcgt tacaaaagag
     4501 atttcttaga aaatttattg gaaggtgtat ctcttgattt aaaaaaatac cttgatgttc
     4561 aagtaaaatt tttatcagga ggtcaaagac aatcattatc actaattatg tcgtgcttaa
     4621 caaatccatc agttttatta cttgatgaac atacagcagc acttgaccca aaaacatcaa
     4681 atgaagtaat tgaattgact aataagattg taagggaaaa aaatataact acacttatgg
     4741 ttactcacaa cttaaaacac gctcttcatt atggtgatag attgataatg cttcataagg
     4801 gtgaagttgt attagatgta aaaggtaaag aaaaagaaca attaacagtt gaagaaatct
     4861 tagaaaaatt tgaatatgca gtttaaaata atatatctat tgtattaaac tcggctttaa
     4921 aataaagtta aaattacaat aattatggtt actttgtaat aattcatatg aatatatgaa
     4981 catatataac aataaatttt tgagggggat ataaaaatga ttaataaaaa aagattggca
     5041 agtttgattt tagctggtgc tcttagtata tctatgctta cagggtgttc acaaggagga
     5101 gactctggta attcaaaaca agaaagtaat tctaaaggta aagaagttaa aaaaattggt
     5161 ataactcaac tggttgaaca tccagcatta gatgccacta ggacaggttt tgtaaaagca
     5221 ttagaaaaga acggatttaa agatggagaa aatattgata tagacttcca aaatgctcaa
     5281 aatgacatgc ctactacaca aagtattgcg agtaaatttg catctgataa gaaggactta
     5341 atatttgcta tatctactcc atcagctcag gcagcattta atgctactaa agatatacct
     5401 atacttatta ctgctgtgag tgaccctgtt gctgctggat tagttaaaac tcttgaaaag
     5461 ccaggcacaa atgtatctgg aacatcagat tttgtttctg tagacaaagg tttagaactt
     5521 cttaaaatat ttgctcctaa ggcaaaaact ataggagtta tgtacaatac tagtgaagta
     5581 aattccaaag ttcaagttga tgctttaaaa gaatatgcat ctaaaaatgg atttaaagtt
     5641 gttgaaaaag gtataactac ttcaaatgaa gttaaccaag gtatttctag tttagttggc
     5701 aaaatagacg tattatatgt ccctacagat aacttagtgg cttcttctat gcctatagtc
     5761 tctaaaatag ctactgagaa taaaatacct gttatagcag ctgaatctgg tccagttgaa
     5821 aaaggtgctc ttgcttgtca aggtatcaac tacgaaaaac taggttacaa aacaggtgaa
     5881 atggcagtta aaattttaaa tggcgaatca gtatctgata tgcctgtggc tacatcagat
     5941 gatacagata tcattgttaa tgaagatata ttaaaagctt taggtatgga aaaaccttct
     6001 aatgaaaata tttcttatgt aaagactaaa caagaataga aagatatttt ctatatacga
     6061 ttaatatgcc tgtttctata aattaattta tagaaacagg catatatttt tattttaatg
     6121 tattatctat ttaaagatta tatgtaaatg ctcttaattt gtttgtataa attttacttg
     6181 tataaacttt atacaatcat atttattctt ctattttctt tattactcta catggatttc
     6241 caacagctac agtatttggt ggtatatctt ttgtgactac acttcctgca cctattacta
     6301 cattatcacc tatagtaatt cctggtgtta tgattactcc cccacctatc catacattat
     6361 cacctatttt tataggagaa ccatactcta tgcctgaatt tctaagttgt gcatcaattg
     6421 gatgataggc tgtatagatt tgaacgtttg gagctaacat tacattgtca cctatttcaa
     6481 ttttacatac atctaaaaaa atacaatcat aatttgcaaa gaaattttcg cctacatgta
     6541 tgttatatcc ataatcacac ctaatatttt gttctacatt tatttgcttg ccaactgaac
     6601 caaatagttg tcttaaaata tcttctcttt tttcatattc atcctctaaa gtattattaa
     6661 acaatctagt taatttttta caatactctc tttcttttac taacaattca tcatttgcat
     6721 agtatccttt cccagacaac attttttctt tttcagtcat atagtttact cctttttgtt
     6781 ataattgtat ctaaagcaaa ataaatattc ctgtggaaaa tctaaatata ttctgtcacc
     6841 tttcttaaat ctcattttac ttttttccaa ttctatttga ataggtacac attcgttaga
     6901 taaatcagtt tttctaacat atatagtata tgtaaaagga ttttctacaa tgttaatcaa
     6961 ttcgaaacat aacttttcat ttaaatcttc attagaatcc aagaccttaa tatgatgctc
     7021 cctgattccc acatattcta tattatcacc tatttctcta tttaatgtca actcgcatcc
     7081 ccagtctgta gcataaatag tatttttatc cagtctattt aatttggata tatttttaca
     7141 ccctgttatc ttagcttcta taaggtactt tggagattca aagagctctt ttttaggtct
     7201 ctttggaaga ccttctcctt tgttgtacac tataatatca tcacaaactc ggtaagcttc
     7261 ttcaatatca tgagtaacaa atagtatatc tcccttgtaa tcttttaaca tattcataag
     7321 ttctttttcc atattgcttc ttaaatgatg gtccaatgct gagaaaggtt catctaaaag
     7381 taatatatct ggagatgttg ccaatgctct agctagtgct accctttgtt gttgaccacc
     7441 agataattgc caagggtatc tcccctcaaa cccatttaat ttaagtatat ctaagtacct
     7501 tgctactatt tccttttttt cactcttact tagttttaaa agcccaagtt ctatattttg
     7561 acttacagtc atatgtggaa acaaagcata gttttgaaat aaaaatccta cctttctatc
     7621 ttgtgttgat aaatttatct tcttttcaga atcaaataga actctatcat ttaatacaat
     7681 tttacctctt gttggtttct caagccctgc tatacatttt aatgtcatac tttttccaga
     7741 accagattct cccaaaaacc ccagagtacc tttttcttgt ttaatttcta cctttagttt
     7801 aaaagaggat aaatcttttt caatatccac gtataagctc aactatccac acctctttcc
     7861 tataattttc tgctgaaatt ccgaccaata atttagtaat aaaatcattg ctatagatat
     7921 tgctacaata atcataaccc acatcatagc tttattcata tctccgcctt caacagcaaa
     7981 aaatatagct attggcatag tttgagtttt tccaggtata tttccagcta tcataagtgt
     8041 agcaccaaat tctccaagag ctctggcaaa cgacagcacc aatccaccaa ttattccagg
     8101 ccatgctaat ggaactgcaa tcttaaaaaa tactttccat tcattaagac ctaatgttct
     8161 agcagctgac aacatattat tatcaatctg ttcaaaagct gaccttgaag ttctatacat
     8221 cataggaaaa gacaccacta ctgctgaaat tacagttgct gtccaactaa atattaaagt
     8281 cctatcgaaa tttaataaaa actttcctat aaatccattc ttcccacata gtaataataa
     8341 aaagaaacct acaactgttg gtggcaaaat tagtggaagt gtaaatagtc catctataag
     8401 acccttccac ttacctctat agttagccat aatatatgac acaaatattc ctattacaaa
     8461 agttattatt gttgacaaaa tagatgtctt aagtgatatc cataagggtg accaatctgt
     8521 tcccatcttt ccctccttaa ttttaaatgg aaatagtgaa actaataact ctattaattt
     8581 cactatttcc attttattat aatacacaat tattttcaat tttcatttat ttatatataa
     8641 tttatactta attacacata atttatttct aagtttattc tactttttta tatccaaatt
     8701 cttcaaaaat cttttgtcct gactcactta ataaaaattc ttcaaatttc ttagcagcat
     8761 ctacgttttt actagcttta attactgaaa ctggatatgt aataggcgaa tgagtttttt
     8821 cgtctgtttt ttcaactact tttatcttat cattattgac agtatcactg aagtaaacga
     8881 atccaacatc agcatttcct gattgcaccc atgctaaaac ttcttttaca tcttttgcaa
     8941 atactagctt atcttttaac ttatctttta aatttaaatt agttaacacc tcatctgcat
     9001 acttaccagc tggaacactt tcagcttcac caacagcaat tttcttcact ttatctgtag
     9061 tcaaatcttt catacctgat acactactgt cagcagaaga aataagaact aaatcatttt
     9121 ttactaaatc tttcatagta tcagaaacta ataacttctc ttcatccaaa gctttcattt
     9181 gtttttgacc tgctgaaata aataaatcac aaggtgctcc ttgttctatt tgttgttgta
     9241 atgagcctga tgctccatag ttaactgtaa gttttacatt tgaatcaact tttttatatt
     9301 cttcttcaat ctttgccatt gcttctttta aacttgcagc agccgatata tttaactcta
     9361 ctgaatcact acttttagtt gttttttctt gttttccatc atcttttttg tcttcattac
     9421 tattgcaccc aactgtcccc aaagttaaac atgcaactaa tcctaatatt cccaatattt
     9481 ttttcataac aatctccctt tttgtaaatt tgatacacat tttaacatgt tttattatgt
     9541 tttatcatgt tttgttatat atagtttaat tatatataca tgataataaa ttttcaagta
     9601 atttatttac actactttta aatattttgc tacattttcc tttttttaat gttaaaataa
     9661 ccttatatgg aggtggatta tggactcatt atcatctttg acacctgtcg aggtagctga
     9721 attattaaag ataacaaaaa atactgtata tgaattaatc aaaagaggtg aacttccttc
     9781 ttataaagta gggaaaaaaa taagagttga tactaaagat gttgaagaat atatcaacag
     9841 ccaaaaaact ggtaaagtaa aggatacaca tttcaacaat aaaaatatag atataaataa
     9901 taatttcaaa attattattt ctggtcaaga tgtaatatta gacattttag ctaggtctgt
     9961 tgaaaaaaaa ttggatggaa taagtacatt tcgctcttat ataggtagtt ataatgcttt
    10021 gtatgagtta tataatggca gagtttctat agcatcatcc catctatggg atttcgaaac
    10081 ggatgaatat aatagcactt ttgttaaaaa gctattgcca ggaataccat gtgtacttat
    10141 aaatctagct tatagaatgc aaggtttcta tgttgctaaa ggaaatccta aagagataac
    10201 aacctgggag gatttaacaa aaccaagtat aaccattgta aatagggaaa aaggttctgg
    10261 aactagggtt ttactagatg gtaaacttag acttctaaac tttaatggta aacatataaa
    10321 aggttatgat aatgaagaat tatcacatct tggagttgca agtactgttt ctcgtggaat
    10381 agcagatgtt ggtttgggaa atgaaaaagc tgctttacaa gtaaataata tcgactttat
    10441 tcctcttcac aaagagcgct atgatttagt tattaaaaaa gaagacttac aaaatccagt
    10501 ttaccaaact ataattaaca taataaactc tcctgagttt aaagctgaac ttcaaggtct
    10561 tggcggatat gatttaactg atactggaaa aactatagca aaagtttaag attaaaatcg
    10621 caatctattt tataagattt ataatttttc acaaaattta taacttaaac acgaaataaa
    10681 ttgcgatttt agtgtctcaa aaaatttatg tctttcattt tatcatccaa tacaaaatat
    10741 aatataatta attgtgcaat catattatat taatatatat ttacataata gtatttttct
    10801 ttaatgtaga ttaacatatg atatgtttta aacatcaatc cccttgcttt cttatatata
    10861 tttggcaaca catatgagtt taataaatta tatacaatgt tgctttcttc ttgtattttt
    10921 tcaaccaaca agttatttat aaaattacct ttctcatttt caataatctc actccaattt
    10981 atcttaactt ttaatatata tcttcccact aggtatacac actctacaat aaataataaa
    11041 aatacaatgc agtcagtacc tgttgtctta tataaattac ccgatactaa tggctttgcg
    11101 aagaaattat aaaaattcaa agtatcaccc gtataatcaa aaagtattaa tagcactcct
    11161 agaactctca atactttaat attcaacatg tttttatctt tattattttt gtttttcata
    11221 tatactacct ctcttttctt tagatttgat tgtaatctaa tactaactct tttatttgag
    11281 gtataaatga atttaattgg tagttatttt catttttact agaaattaat tcacattact
    11341 aaacaaaact tatatttaaa cataaaaata gcctatctat tttttaataa atacaaaaat
    11401 aaataagcta ctaatattca attaactttt atcacttaaa acattactat ctataaattt
    11461 aaatatttta ttgaagtata ctgtactatc atgttcaaat ccatcaacat gaccaatacc
    11521 ttctataagc cataggtctt ttttatcatg tttaactaaa tcatacattt tcttgctatt
    11581 ttgataatta caaacagtat cttttgttcc atgtataaac agtatgggta cattagattt
    11641 ctcaactgac tcatatggtt ttacatcact gtaccaaaat ccagatttaa acttattata
    11701 taaatctcca caagtcacca cataattgat taatatatct ggtattcgtt tttctagtac
    11761 tcccattcta attgcatctt tcatttcaga ataaggagaa tccagtatat aaaaatcgac
    11821 cttatcatct tttgaattta tctctgaatg catagcagct gtccctgccc ccatggaaaa
    11881 tccatgcact cctaatcttc cttcaggaaa tttatttttt acaaatttaa ctagactatc
    11941 taaatcatac ctttcataaa gaccaaatgt gtaattatct ccaccactat taccagtatt
    12001 tctttgatta taaactaata cgttataacc tttatctaaa tatctatatg ctacctttaa
    12061 catttcatag taactacttc caattccatg aacaagtatc atagtatctc ttgtctttat
    12121 atttgaagtt ataaaaagac tttcaagctt gtaattatta gaactttgta tcattaattc
    12181 agatgtcttg tagtttttta atttctccag tggtctatct tttctctcag aaaatatttt
    12241 tactatatct tcgtttttta ttaactgctc taaaccaact gaactattat acactaattt
    12301 gccaaatata taagatacaa ttaataggat taatagaaac acaattgata taattataaa
    12361 tttatttctt aaactaatac aatttttaaa actatttttc atatttattt accatacaat
    12421 tttcctatat tatattttct aagtaatagc ataattgatt atactattct tataatttct
    12481 attataccta cttat
//
LOCUS       DAYAPA010000124        12369 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.126, whole genome shotgun sequence.
ACCESSION   DAYAPA010000124 DAYAPA010000000
VERSION     DAYAPA010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12369
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.126"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            308..670
                     /locus_tag="ACGI59_003129"
     CDS             308..670
                     /locus_tag="ACGI59_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFG9740905.1"
                     /translation="MENKILIVDDAIFMRMIIKEALSKSGYNNLIEANDGEEACNIFK
                     YEKPDLMLLDITMPKKDGLEVLREIKKLDSSAKVVMCSAIGQENMIVEAIKLGALDFI
                     VKPFKTETLVKVVDNALK"
     gene            768..1352
                     /locus_tag="ACGI59_003130"
     CDS             768..1352
                     /locus_tag="ACGI59_003130"
                     /inference="COORDINATES: protein motif:HMM:NF016398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheC"
                     /protein_id="HFG9740906.1"
                     /translation="MDALREIGNIGSGNAITALASMINSNVEVLIPMVKILEYNEATN
                     LLGGPENKVVCILLDMKGDINGMFMFLLDESITQLMLSSLFNKEEAFLDEIEAIEISA
                     IKEIGNIMASSYVNAIASMLNMTISVSIPDICIDMVGAVLNVPMIRFSDVGDKVLFIE
                     NKFKMSDNYFTSHILMIPEMSSLREILVRLGLEV"
     gene            1349..1834
                     /locus_tag="ACGI59_003131"
     CDS             1349..1834
                     /locus_tag="ACGI59_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HFG9740907.1"
                     /translation="MNREIVVNIADMKIAYRPNVLVTYALGSCVGVCLIDKVAGIGGM
                     LHVMLPYSKDAINIENKCKFADTGINELIKSMENIGANRIYIKAKIAGGAQMFLGSSN
                     SVIASIGHRNVEAVKKTLLGLNIPILAEDTGMNYGRTIRFYTETGELLVDSINKGKKK
                     L"
     gene            1877..2395
                     /locus_tag="ACGI59_003132"
     CDS             1877..2395
                     /locus_tag="ACGI59_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HFG9740908.1"
                     /translation="MRFPDDPYLDYGLIDEGIKYLKFKVDEQDFGVELEKVIEITGNQ
                     EIIFIPELPTYSKGIINLRGKIIPIIDMRLRLKKDELMTTNCMYIVVTEIKDLVVGFV
                     VDRVDDIVSLEKSKISPPPRVTIEYSDYFVSGVGELEGKIITLLDLEKLLTDKDEVLI
                     DELINGFDCTIM"
     gene            2531..3490
                     /locus_tag="ACGI59_003133"
     CDS             2531..3490
                     /locus_tag="ACGI59_003133"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFG9740909.1"
                     /translation="MKNKQQGTILVVDDSVLMCDLIDKTLTKDNFKVKKAFNAIEAKE
                     FIEEFIPDIILLDIILPDNSGFEFCKEVKSNARTADIPIIFITSKNTDTDIVKGFGVG
                     AIDYMVKPFSMTELKARVIAHLEVKKSQDKLKKINNELESSLEKLNRLVVRDYLTGLY
                     NRRHIINQLMEQRRINKYSETSISLILGDIDDFKVINDTYGHEAGDFVLTVISSIIKK
                     NCRQIDTVSRWGGEEFLIMLTNTPIEGAKILSERIRKEIKEFDFNYKEHKIKCTITLG
                     VVEVNSNISLEENISLADKAMYEGKNLGKDCSVVSTMKGLKKI"
     gene            3516..5219
                     /locus_tag="ACGI59_003134"
     CDS             3516..5219
                     /locus_tag="ACGI59_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HFG9740910.1"
                     /translation="MKREVKLSKLAIVLLIIIVLAVLAGIFSSISSYKAFEKVEKSIN
                     DMNNLRTQSQYIKDTTREISDVLRRYVFTGEKKNKDIYEKELYGKTRESALNKLEKIG
                     LTKSESNKILEAKEISDSLVPTEQSAIKAVESNDKSKAQSIIFGSDYINSQEKMDSLI
                     NEFQEDIVTRSNSKIVEVKKELQSLILRTIFILSLLIILTIIEYTVIKLKIVIPINKV
                     EKHFSRIASGDLRTSIDVEESKSEIGLLVSSVKKMQSMLLNYIDLIDSTLTSIAKGDL
                     RVEIDQEFIGDFKSIKISLESIINSFNGDFSEINLSAEQVASASEQVAAGAQALSQGA
                     TEQASSVEELASTINEISDGIKKCADSASLACEVSDQSASQVGFGSRCMDDMMTSMGE
                     ISEKSSEISKIIKTIDEIAFQTNILALNAAVEAARAGQYGRGFAVVADEVRSLAGKSA
                     KAAQNTAILIEEAIKTVENGVRIAKETANALDLVVNGVEKTTNYINGISSAVHSQEVA
                     ITQILSGIEQISIVVQMNSATAEESAAASEEMSAQAQILKELVEKFHLKESEVRESSW
                     V"
     gene            5480..7582
                     /locus_tag="ACGI59_003135"
     CDS             5480..7582
                     /locus_tag="ACGI59_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HFG9740911.1"
                     /translation="MRQMLDMFIFETSLLLEQLDEIMIQIEKEKVFTHNNINETFRIM
                     HTIKGSASMMGLDNISALAHSVEDLFYAIREGKIEVLDNDLLFDLVFQSLDFIRRDID
                     ELQSRGCISSDYSNFIEGIEEYVSTLELSSSMKSKDNANLESKDEKEVIDSNFEYKED
                     SDVVKIIFEQGCMMENVRAFMLVFELRDYCEFIDFYPKDVETNAESTEYIKSYGFYIQ
                     FVAKEDKAIVYKTIEDNLYVKEYINISKKEIEMKSTSNNDAVENKLKDNNKVSKSYQN
                     NYTEDEIQEDVLNEKTDMEKDSLSEENEHLPISKQSIMSVNLEKLDLLQNIVGEIVIM
                     ESMVVNSVNSTGVDIDNFNKATRQLHKLTDELQDIVMSMRMIQIAGVFQKMHRIVRDM
                     NKKLDKNVELITIGAETEVDKSVIDNLAEPFMHLIRNAMDHAIEPIEERIAKGKEEVG
                     KIVLSAENLGGEAVISISDDGRGIMRDKILAKAKEQGLLKHQSNEYTDDEVNSLIMTP
                     GFSTKESITEFSGRGVGMDIVRKSIEKVGGSIEVKSKQDEGTTFTIKIPLTLSIVEGM
                     KFKVGDSLFTLPISSIRRTFKLTDLNQIITEGNKTELITIYGACIPIIRLHKIYNIQT
                     KVTNLMNGNMIIIENEHKAVCIFVDEIIGNQQVVVKPFPVYFNRFNIKNKGLSGCTIL
                     GDGSISLIIDADSIIEIY"
     gene            7595..8035
                     /locus_tag="ACGI59_003136"
     CDS             7595..8035
                     /locus_tag="ACGI59_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HFG9740912.1"
                     /translation="MAKDEIIKQVLTFYVNDVIYGIELENVIETIRFQSITYVPCLPT
                     YISGVINLRGRIIPVINMHIKYNLPKADYNERTCIIITKVDEYQVGIIVDKVVDVIHI
                     ENLNLLETTNSNNTNINKDIKEIAKVEDNSILILDIRKFLINSI"
     gene            8071..8874
                     /locus_tag="ACGI59_003137"
     CDS             8071..8874
                     /locus_tag="ACGI59_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HFG9740913.1"
                     /translation="MIKLTDEEFIVLVNHVKKEYGIDLSKKRALIEGRLYNTMIEKKL
                     SSFSQYMNLLFKDRTGNEAINLINRLSTNHTFFMREPQHFEFIQNSILPFWEENNKIR
                     NINIWSAGCSSGEEAYSIAMTLDDYFGYNKELWNIKIIATDISTNSLEKAKRGIYIES
                     NVNNVPELWKKKYFIDNKDGTFKICDKIRKSVIFKPFNLMNDFSYQHFDLIFCRNVMI
                     YFDLKTREELINKFYNATKKDSFLFVGHAEVINRSSTKYKYVKPAIYKR"
     gene            8914..9510
                     /locus_tag="ACGI59_003138"
     CDS             8914..9510
                     /locus_tag="ACGI59_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheB methylesterase domain-containing protein"
                     /protein_id="HFG9740914.1"
                     /translation="MERFNNMVIAIGASVGGTEAILEIIKDLPKSTPGIVVVQHMPAI
                     FTYMYAQRLDKQCIMNVREAKNNDRVEQGNVLIAPGGYQMKLCTDKQGYYVTCEKGER
                     ISGHCPSVDVLFDSVAEVAGKNSIGIILTGMGYDGASGLLKMKENGSFTIGQDENSCI
                     VYGMPMVAFDRGSVMLQLPLGEISNCLIRHINILKQKD"
     gene            9616..10479
                     /locus_tag="ACGI59_003139"
     CDS             9616..10479
                     /locus_tag="ACGI59_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HFG9740915.1"
                     /translation="MKFFKVDTKAKVLRYVFVLTLTIMAVLAIKHDYKYNNLRKDASI
                     NLNSNKEAVTVMSNQSLPKGNTNSKQTLSVSTIGSKTIVKNDEYLESTINMPVITNSN
                     KIVERSTNDKIKNDIFEFYNKSYKEAKQYLKDNPDEKNKFVANVDFELKKNTDSALSI
                     KVRYYTYSGGAHGFYQDIAYNVDMRTGKFLELMDLFKDNTKYKEVIDEEIKRQIAELE
                     KKDEENIGIYNFKGIKENQNFYLQDENLVVYFDLYDITPYAAGIPEFSINKALISSML
                     KPEYVDLFDLK"
     gene            10546..11535
                     /locus_tag="ACGI59_003140"
     CDS             10546..11535
                     /locus_tag="ACGI59_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HFG9740916.1"
                     /translation="MLLLKKDDIKKVFTMRDAIEADKEAFRIYCEGKSVNPLRTNISV
                     PSQDASMLFMPGYVEELGCGGIKIVSVFPKNAQKGKPVIPATVLLLDGETGEVSAVLD
                     GTYVTQIRTGAASGAAIDVLAKKDSKIGALIGLGGQGEPQLEAMIEARNLDEVRVFSR
                     NKESREKFAEEMNLKLSKYNTKIVAVESSDEAIDNADVIVLATPSKQPVLNGNLVKKG
                     ALISAVGSYMPDMQELCPNCLTRASKIFFESTDAVLSESGDILIPLKEGKISKDDFSG
                     DLGNVINGTIPGREDDDEIIVFKTVGIGVQDVVTAKRIYDKAKENGIGIDWKW"
     gene            11627..12238
                     /locus_tag="ACGI59_003141"
     CDS             11627..12238
                     /locus_tag="ACGI59_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFG9740917.1"
                     /translation="MKMVKKLILSTIMALVLVLTIGCSNAEEKVKSNFEANLAALEMY
                     KYDEKGISDALTEAEYKQIQTYNSKDKVSMIAQLTPLYGKENAEKIYETIVKLIQGVE
                     KTVTVESSSKDKITLKVVSKALDTSKVMSEANTLVKSYVTDEKNYDKKEKEVQDDMTK
                     MIIDLYNKKPTKEVTTMITYTKKDGKWQPPSNIQKLCEGIYTI"
BASE COUNT         4996 a         1212 c         2327 g         3834 t
ORIGIN      
        1 ttttttatac aaacataaaa aaggtataaa gttgttttat tataatttaa ttacttttga
       61 taacttttta tgttttatac aattatattt ttaataactc gtcaaaacgc caaacatata
      121 ttacaataga tatataagtt tttttgttga caaattatgt taagagaatt aagtataata
      181 tggcataaat aattactttt atttatcaat gaaatgtatt tattgtgtag cgaaaaatag
      241 tcagatagta tagacaataa ctgaagataa aagcgtttat tgttgtacat atggaggagt
      301 tagttttatg gaaaataaaa ttttgatagt agatgatgct atttttatga gaatgataat
      361 taaagaagct ctgagtaaaa gtggctataa taatttaata gaagcaaatg atggagaaga
      421 agcgtgtaat atatttaaat atgaaaagcc tgatttgatg ttgcttgata ttacaatgcc
      481 aaaaaaagat ggtttagagg tgctaagaga aataaaaaag ttggattcaa gtgctaaggt
      541 tgtaatgtgc tctgcaatag gacaagagaa tatgattgta gaagccataa aacttggagc
      601 gttagatttt atagtgaagc ccttcaaaac agaaacatta gttaaagttg tagataatgc
      661 tttaaaataa aagtgtaaat atacactaat acaatgaaaa gcaaataaat gcaataataa
      721 agggaggggt ttatattata aattatttag aacttgatga tgttcatata gatgctctta
      781 gagaaattgg taatataggc tctgggaatg caattactgc acttgcatcc atgataaata
      841 gcaatgtaga agtattaata cctatggtca agatacttga atataatgaa gcgacaaatt
      901 tgcttggtgg tccagaaaac aaagtagtat gtattttact cgatatgaaa ggtgatatca
      961 atggaatgtt tatgtttttg ttggatgaat caataactca acttatgcta agttctcttt
     1021 ttaataaaga agaagctttt ttagatgaga ttgaggcaat tgaaatttca gcaattaagg
     1081 aaataggaaa tattatggca agttcatatg taaatgctat tgcatctatg ttaaacatga
     1141 ctataagtgt ttcaattcca gatatatgta ttgatatggt tggagctgtt ctaaatgtac
     1201 ctatgataag attttcagat gttggtgaca aagtcttatt tatagaaaat aaatttaaaa
     1261 tgagtgataa ttattttact agtcacattt tgatgattcc agaaatgagc tcacttagag
     1321 aaatactcgt gaggttggga ttagaagtat gaatagagag atagtagtga acattgcaga
     1381 tatgaagata gcctatagac caaatgtttt agttacttat gcacttggtt cttgtgtagg
     1441 agtatgtttg atagataaag tagctggaat tggtggaatg ttacacgtta tgttgccata
     1501 tagtaaagat gctattaaca ttgaaaataa gtgtaaattt gctgacacag gaataaatga
     1561 gctcattaaa tctatggaga atataggtgc aaatagaata tatataaaag caaaaattgc
     1621 tggaggtgct cagatgtttt taggaagtag caattcagta attgcaagta taggacatag
     1681 gaatgttgag gcagtaaaaa aaactctttt aggattaaat attccaatat tagctgagga
     1741 cacaggtatg aattatggta gaacaatacg tttttacact gagacgggtg aattacttgt
     1801 agattcaata aataaaggta agaagaaatt ataatatata tttcatagaa agtgtatttt
     1861 aggagggagg tattaaatga gatttccaga tgacccatat ttagattatg gactaattga
     1921 tgaaggaatt aagtatctaa agtttaaagt agatgagcaa gattttgggg ttgaacttga
     1981 aaaggtaata gaaattactg gaaatcaaga aattatattt attccagaac tacctacata
     2041 ttctaaaggc ataataaatc taagaggaaa aatcattccg ataatagata tgcgtttgag
     2101 acttaaaaaa gatgaattaa tgactacaaa ttgcatgtat atagtagtaa ctgaaataaa
     2161 ggacttggta gttggatttg tggttgatag agttgatgat atagtaagct tagaaaaaag
     2221 taaaatatca cctccaccaa gagttactat agaatattcg gattattttg taagtggagt
     2281 tggagaatta gaaggtaaaa taattacttt actagatttg gagaaacttt tgacagataa
     2341 agatgaagtt ttaatagatg aattaatcaa tgggtttgat tgcactatca tgtaacatag
     2401 ggatgtagtt ggagtgttat tataaaaaga ttgtgatgag aacatttaag aatatagtgg
     2461 tataattatt aagtataaaa attttaaatc attaaaattt gaatatatta tgtttaagag
     2521 aggtttgtat atgaaaaata agcaacaggg aacaatttta gtcgttgatg atagtgttct
     2581 gatgtgtgac ttaatagata aaacacttac aaaagataat tttaaggtta aaaaagcatt
     2641 taatgcaata gaggcaaaag aatttattga agaatttata ccagacatta ttttgcttga
     2701 tataatttta ccagataact ctggttttga gttttgtaag gaagtaaaat ctaatgctcg
     2761 tacagcagat atacctataa tttttataac ttcaaaaaat acagatacag acatagtaaa
     2821 aggatttggt gtaggagcaa ttgactatat ggtgaaacct tttagtatga cagaattaaa
     2881 agctcgtgtt atagctcatc ttgaagtaaa gaaatctcaa gataaattaa aaaagataaa
     2941 taatgaactg gaatcttctc ttgaaaaatt gaatagactc gttgtaagag attatcttac
     3001 aggtctttac aataggcgac atataattaa tcagcttatg gagcaaagaa gaattaataa
     3061 atatagtgaa acatcaatat cccttatcct tggtgatatt gatgacttta aggttatcaa
     3121 tgatacttat gggcatgaag caggggattt cgtgttaaca gtaatatcaa gtataataaa
     3181 aaagaattgt agacaaattg atactgtatc aagatggggt ggagaagagt ttttgataat
     3241 gctcacaaat actccaatag aaggtgctaa gatactttca gaaagaatcc gtaaagagat
     3301 aaaggagttt gatttcaact ataaagaaca taaaataaag tgcactatta cattaggagt
     3361 agtagaagta aactcaaata tatcattaga agaaaacata tccttagcag ataaagcaat
     3421 gtatgagggt aaaaacttag gaaaagattg tagcgtagtg agtacaatga aaggattaaa
     3481 aaagatataa acttacttta tgaaggagta cagatatgaa aagagaagta aagctttcta
     3541 aattagcaat tgtattgctg attattatag tattggctgt tctagcaggg attttttctt
     3601 ctatttcatc ttataaagca tttgaaaaag tagaaaaaag tattaatgat atgaataatt
     3661 tgaggactca aagtcagtat ataaaagata caactagaga gataagtgat gtattaagaa
     3721 gatatgtatt tacaggagaa aaaaagaata aagatattta tgaaaaagaa ttatacggaa
     3781 aaacaagaga atcagcttta aacaaattag aaaaaattgg tcttacaaag agcgagtcaa
     3841 ataaaatctt agaagcaaaa gaaatatcag attctttagt accaacagaa caaagtgcaa
     3901 taaaggcagt cgaaagtaat gataagagta aagctcaaag tataattttt ggtagtgact
     3961 atataaactc ccaagagaaa atggattctt taataaatga atttcaagag gatatagtta
     4021 cccgttcaaa ttccaaaatt gtagaagtaa aaaaagaatt acaaagtcta atattaagaa
     4081 caatatttat actttccctt ttaataatat tgacaataat agaatataca gtaataaaat
     4141 taaaaatagt gattcctatt aataaagtag aaaaacactt ttcaagaatt gcatctgggg
     4201 acttaagaac aagtatagac gttgaagaaa gtaaatctga aatagggcta ttagtgagtt
     4261 ctgtcaaaaa gatgcaaagt atgcttttaa attacataga ccttatagac agtacattaa
     4321 ctagtattgc aaaaggagat ttaagagtag agatagacca agaatttata ggtgatttta
     4381 aatcaattaa aatatcttta gaaagtataa taaactcttt taatggtgat ttttctgaaa
     4441 taaatttatc agcagagcaa gttgcaagtg cttcagaaca agttgcagca ggtgcacaag
     4501 ctctttcaca gggtgctact gaacaagcta gttcagtaga agaattagct tccacgatta
     4561 atgagatatc agatggaatt aagaagtgtg cagatagtgc tagtcttgca tgtgaggtat
     4621 ctgaccaatc tgcatcacaa gttggttttg gaagtagatg tatggatgat atgatgacat
     4681 caatgggtga aataagtgaa aaatcgagtg aaatatcaaa aataatcaaa acaatagatg
     4741 agatagcatt ccaaactaat attcttgcat taaatgcagc tgttgaagca gcaagagcag
     4801 gtcaatatgg tagaggattt gcagttgtag cagatgaagt taggtctttg gctggtaaaa
     4861 gtgcaaaagc agctcaaaat acagctattc tcatagaaga agctattaaa acagtagaaa
     4921 atggtgtcag aattgctaaa gagactgcaa atgcattgga cttggtagta aatggtgtag
     4981 aaaaaactac taattatatt aatggtattt cttcggctgt acatagtcaa gaagtagcaa
     5041 ttacacagat tttatctgga atagagcaaa tttccatagt agtccaaatg aactcagcaa
     5101 cagcagagga aagtgcagca gcctcagaag agatgtctgc tcaggctcaa attttaaaag
     5161 aattagtaga aaaatttcat ctaaaggaaa gtgaagtaag agaatcatca tgggtttaaa
     5221 ttatgaggtg aatattgaag taagagaatt atcaaggatt taaattatga gatgtatata
     5281 tattgaaata agagaattat caaggattta aattatgaga tgtatatata ttgaagtaag
     5341 agaataattg tgtatttaaa ttatgaggta catatagtga gataagagaa tcatcaagga
     5401 tttaaattat gagatgcata tataatagaa aattatttgt aggtcgtagg tgataaatgt
     5461 gaattatatt gaaccgagta tgagacaaat gttagatatg tttatatttg agacaagttt
     5521 attactcgaa caattagatg aaattatgat tcaaatagaa aaagagaaag tatttactca
     5581 taacaacatt aatgaaactt ttagaataat gcatacgata aaaggttcag catctatgat
     5641 ggggttagac aatatatctg ctttagcaca tagtgtagaa gacttgtttt atgctatacg
     5701 tgaaggtaaa atagaagttt tagacaatga cctattattt gatttagtct ttcaatcatt
     5761 agattttata agaagagata tagatgagtt acaatcaaga ggttgtatat cttctgatta
     5821 ctctaatttt attgaaggaa ttgaagagta tgtgagtact ttggaattat caagttctat
     5881 gaaatcaaag gataatgcaa atttagaaag taaagacgaa aaagaagtaa tcgattcaaa
     5941 ttttgaatac aaagaagact ctgatgtagt aaaaataatc tttgaacaag gttgcatgat
     6001 ggaaaatgtg agagctttca tgcttgtatt tgaacttaga gattactgtg aatttataga
     6061 tttttatcca aaagatgtag aaacaaatgc agagtctaca gagtatatta aaagttatgg
     6121 gttttatata caatttgtag ctaaagagga taaggcaata gtttataaaa ctatagaaga
     6181 taatctctat gtaaaagaat acataaatat tagtaaaaaa gaaatagaaa tgaaaagtac
     6241 atcaaacaat gatgcagtag aaaataaatt aaaagataat aataaagtaa gtaaaagtta
     6301 tcaaaataac tatacagaag atgagataca agaagatgtt ttaaatgaaa aaactgatat
     6361 ggaaaaagat agtctaagtg aagaaaatga acatttacca ataagtaagc agagtattat
     6421 gagtgtaaat ttagaaaaat tggacttact tcaaaatata gttggagaaa ttgtaattat
     6481 ggaatcaatg gttgtaaata gcgtaaattc tacaggtgtt gatatagata atttcaataa
     6541 ggcaacaaga caattacaca agcttacaga tgaattgcaa gatatagtaa tgtctatgcg
     6601 tatgattcaa attgctggtg tatttcaaaa aatgcataga attgtacgag atatgaataa
     6661 aaaattagat aaaaatgtag agttaataac tataggagca gaaacagagg tggataagag
     6721 tgtaattgat aatttagcgg aaccttttat gcaccttata agaaatgcta tggaccatgc
     6781 aatagagcct attgaggagc gtatagcgaa aggtaaagaa gaagtgggaa agatagtttt
     6841 atctgctgaa aatctaggcg gagaagctgt gataagtatt tcagatgatg gtagaggcat
     6901 tatgagagat aagattttgg caaaagctaa ggaacaaggt cttcttaaac atcaaagtaa
     6961 tgagtatact gatgacgagg tcaattcttt gattatgact ccaggctttt ctacaaaaga
     7021 atctataact gaattttctg gaagaggagt aggcatggac attgttcgta aaagtattga
     7081 aaaagttgga ggttcaattg aagtaaaaag taaacaggat gaggggacaa cctttactat
     7141 aaaaatacca ttaactcttt ctatagtaga gggtatgaag tttaaagttg gagattcact
     7201 atttacttta ccaatatcat ccataagaag aacatttaaa cttacagact taaatcaaat
     7261 aattacagaa ggaaataaga cagagttaat tacaatatat ggagcatgta ttcccattat
     7321 aaggttacat aaaatatata atattcaaac aaaagtaacc aatctgatga atggtaatat
     7381 gataattatt gaaaatgagc ataaagcagt atgcattttt gttgatgaaa taataggtaa
     7441 tcagcaagtt gttgtgaaac catttccagt ttattttaac cgatttaata taaaaaataa
     7501 gggtttatcg ggatgtacaa tacttgggga tggaagtata agtttaatta tagatgcaga
     7561 tagcattata gaaatatatt aggggattta aagtatggct aaagatgaaa ttattaaaca
     7621 agtattaaca ttttatgtca atgatgttat ttatggaata gaattagaaa atgtgattga
     7681 aaccataaga tttcaatcaa taacgtatgt accatgtctt ccaacatata taagtggagt
     7741 tattaatttg agaggaagaa taataccagt tattaatatg catataaagt acaatcttcc
     7801 taaagctgat tataatgaga gaacttgtat aataattaca aaggttgatg agtatcaagt
     7861 tggaattatt gtagataaag ttgttgatgt tattcatata gaaaatctaa atttactaga
     7921 gacgactaat tctaacaaca ctaatattaa taaagatata aaagagattg cgaaagttga
     7981 agataattct atattaattt tagatattcg aaagtttttg attaatagca tatagttagt
     8041 tctgataaat tagcaggagg aagtttttgc atgataaaac tcacagatga agaatttata
     8101 gttcttgtaa accatgtaaa aaaagaatat ggtattgatt taagtaaaaa aagagcgtta
     8161 attgaaggtc gcttatacaa tacaatgata gagaaaaaac ttagttcttt tagtcaatat
     8221 atgaatctat tatttaaaga taggacagga aatgaagcta taaacctaat aaatagattg
     8281 agtactaacc acacattttt tatgagagaa cctcaacatt ttgagtttat acagaatagt
     8341 atacttcctt tttgggaaga aaataataaa ataagaaaca taaatatatg gagtgcagga
     8401 tgttctagtg gagaagaagc gtattcaata gctatgactt tggatgatta ttttggatat
     8461 aataaagagt tgtggaacat taaaatcata gccacagata tttcaactaa ctcacttgaa
     8521 aaagctaaaa gaggtatata catagaatca aatgttaaca atgttcctga gttatggaag
     8581 aagaagtatt ttatagacaa caaggatggt acatttaaaa tttgtgataa gattagaaaa
     8641 agtgtaattt ttaagccgtt taatttaatg aatgatttct catatcaaca ttttgatttg
     8701 attttttgta gaaatgttat gatttatttt gatttaaaaa caagagaaga acttataaat
     8761 aaattttata atgctaccaa aaaagatagt tttttgtttg taggacatgc agaagtaatc
     8821 aacagaagta gtactaaata caaatatgta aaacctgcta tatataaacg ataaaaaggt
     8881 atttttgata tatgattttt tggaggcggt aatgtggaac gttttaataa tatggttatt
     8941 gctataggcg catctgtagg tggtacagag gcaatacttg aaattataaa agacctacca
     9001 aaatcaacac cagggattgt tgtagttcaa catatgccag ctatatttac ttatatgtat
     9061 gcacaacggc ttgacaagca gtgtattatg aatgtaagag aagctaagaa taatgataga
     9121 gtagaacaag gaaatgtgtt aattgcacct gggggttacc agatgaaatt gtgtacggat
     9181 aaacaaggat attatgtaac ttgtgaaaaa ggagaaagaa taagtggtca ctgtccatca
     9241 gtagacgttt tgtttgattc agtagcagaa gtagcaggta aaaattcaat aggaataata
     9301 cttacaggta tgggttatga tggagcaagt ggacttttaa aaatgaaaga aaatggttca
     9361 tttacaattg gacaggatga aaattcttgt atagtatatg gaatgccaat ggtagctttt
     9421 gatagaggtt ctgttatgtt acaattacct cttggtgaaa tttcaaactg tttgataaga
     9481 catattaata ttttaaaaca aaaagattaa ttttttataa ataaaaatta atcttttatt
     9541 aaattatatg gataaaaaac cctagaaact ccaaatataa taagagagat ttatatttag
     9601 gaggaaaatg ataatatgaa attttttaaa gtcgatacaa aagctaaagt tttaagatat
     9661 gtatttgttt taacattgac tataatggct gttttggcaa ttaaacatga ctataaatat
     9721 aataatttaa ggaaagatgc ttcaattaat ttaaatagta ataaggaagc agtgactgtt
     9781 atgagtaacc agtcattacc aaagggtaat acaaatagta aacaaacttt atcagtaagt
     9841 acaataggaa gtaaaacaat agtgaaaaat gatgagtatt tagaaagtac tataaacatg
     9901 cctgtaataa caaattcaaa taaaattgtt gaaaggtcta caaatgataa gataaaaaat
     9961 gacatatttg aattttataa taaatcatat aaagaagcaa agcagtatct taaggataat
    10021 cctgacgaga aaaataagtt tgttgcaaat gtagactttg aacttaaaaa gaatactgat
    10081 tctgctctta gtataaaagt gagatattat acttatagtg gtggagcaca tggattttat
    10141 caagatatag catataatgt ggatatgaga actggaaaat ttttagaatt aatggacttg
    10201 tttaaggata atactaaata taaagaagtt atagatgaag aaattaaaag acaaatagct
    10261 gaacttgaaa aaaaggatga agaaaatatc ggaatatata attttaaagg tataaaagag
    10321 aatcaaaact tttacttgca agatgagaat ctagttgtat attttgattt gtatgatata
    10381 actccatatg cagcaggaat accagaattt tcaataaata aagcgttgat aagttctatg
    10441 ctaaaaccag agtatgtaga tttatttgat ttgaaataga agattaaatt aaatgttatg
    10501 ctataatatt tagcatagag acttattaaa attgggggta taaaaatgct acttttaaaa
    10561 aaagacgaca taaaaaaagt atttacaatg agagatgcaa ttgaagctga taaggaagct
    10621 tttagaattt attgtgaagg aaagagtgta aatcctttga gaaccaatat atctgttcca
    10681 agtcaagatg ctagtatgtt atttatgcca ggatacgtag aagaattagg ctgtggggga
    10741 ataaaaattg tatctgtatt tcctaaaaat gctcaaaaag gcaaaccagt aattccagca
    10801 acagtactat tattagatgg agaaactgga gaagttagtg cagtattgga tggtacttat
    10861 gtaactcaga ttcgtactgg agcagcctct ggagcagcta tagacgtatt ggctaaaaaa
    10921 gattctaaga ttggagcttt gataggtcta ggaggacaag gtgagccaca acttgaagcc
    10981 atgatagaag ctagaaattt ggatgaggta agagttttta gtagaaacaa ggaaagtaga
    11041 gaaaaatttg cagaagaaat gaacttaaaa ttaagtaaat ataatactaa aatagttgca
    11101 gttgaatcat ctgacgaagc catagataac gcagatgtga tagttttagc tactccatca
    11161 aaacaaccag tacttaatgg aaacttagtg aaaaaaggag ctttaataag tgcagtaggt
    11221 tcatatatgc cagatatgca agagttgtgc ccaaactgtt taactagagc gtctaagata
    11281 ttttttgaat caacagatgc agttttatct gagtcaggag atatattaat tccattaaaa
    11341 gaaggtaaga taagtaaaga tgattttagt ggtgatttag gaaatgttat aaacggaact
    11401 attcctggaa gagaagatga tgatgagata attgtattta aaactgttgg gataggtgta
    11461 caagatgtcg taacagcaaa aagaatatac gataaagcaa aagaaaacgg tattggaata
    11521 gactggaagt ggtaaaatat tcaaaataat tctatagtta gacaaattta tgttataatg
    11581 ttgataaata aattttttat tattttaatt gagggaggat gtttgtatga aaatggtaaa
    11641 aaagttaata ttatctacga ttatggctct agttttagtt ctaactatag ggtgttctaa
    11701 tgcagaagaa aaagtaaagt cgaattttga agctaattta gctgcattag aaatgtataa
    11761 gtatgatgaa aaaggaattt ctgatgcatt aacagaagct gaatataaac agattcaaac
    11821 ttataacagc aaggacaagg tttcaatgat tgctcaattg acacctcttt atggtaaaga
    11881 aaatgctgaa aagatatacg aaacaattgt taaattaatt caaggtgttg aaaagacagt
    11941 aacagtggaa tcttctagta aggataagat aactttgaaa gttgtaagta aagctttaga
    12001 tactagtaaa gtaatgagtg aagcgaacac tttagtaaaa tcttatgtaa ctgatgaaaa
    12061 gaattacgat aaaaaagaaa aagaagttca agatgatatg acaaaaatga ttatagacct
    12121 ttacaataag aaaccaacta aggaagttac aactatgatt acatatacta aaaaagatgg
    12181 taaatggcaa ccacctagta atatacaaaa gttatgtgaa ggaatatata caatttaatt
    12241 tattttaaag acacaaaact atcttttaag catataataa aatgtttgga agatagttta
    12301 ttttattttc aaagtataaa ttatatttta taaatgattt attttataaa taatttatct
    12361 tgataaata
//
LOCUS       DAYAPA010000125        12214 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.127, whole genome shotgun sequence.
ACCESSION   DAYAPA010000125 DAYAPA010000000
VERSION     DAYAPA010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12214
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.127"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            190..573
                     /locus_tag="ACGI59_003142"
     CDS             190..573
                     /locus_tag="ACGI59_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HFG9740918.1"
                     /translation="MLNRKLPDAELKIMKYIWNTGYKTVISKDVADEIEKTYGWKHTT
                     TITLLARLTKKGFLISQRIGKHVHYTVLVKEREYLKLEGKRIFGGLHNNPLSELISKL
                     HDGEEITEEKIIEIGNWIKSWKDED"
     gene            1336..1746
                     /locus_tag="ACGI59_003143"
     CDS             1336..1746
                     /locus_tag="ACGI59_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9740919.1"
                     /translation="MKTSFQMLFSVISLVNINLLQAQILIAIGGLISILAMVSLTLFL
                     SAKCKDSLTSMLIAFSICLIPMGIGVSWIGYLLPPGGIGFKISLLYQMTSFNFLHLGG
                     MSFWTPHIILFFSVIEIPIFIFLAIHTYCKHQVS"
     gene            2127..3044
                     /gene="pstC"
                     /locus_tag="ACGI59_003144"
     CDS             2127..3044
                     /gene="pstC"
                     /locus_tag="ACGI59_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HFG9740920.1"
                     /translation="MIVKSRLVGLEKNDNNGNKTKYVIEKVAKNIFLLSALVAVASLL
                     LIIGFVFYKGLRPFIFEGYSFIDFLTGADWVPSANKFGISSMIVASIFATLGALVIGV
                     PVGILTSVFIAEVAPKRLAKIMSPAVELLAGIPSVLYGVFGLAVIVPTIQSIFNLPKG
                     QSLLAIVIVLAVMMLPTIISVSETAIRAVPKAYKEGSLALGASKIETIFKVVLPAAKS
                     GVLAAVVLGVGRALGETMAVILVAGNSPVMPTAITDSVRPLTTNIALEMGYAFGTHQE
                     MLFATGVVLFTFILILNLVLNKLSNKAVN"
     gene            3046..3870
                     /gene="pstA"
                     /locus_tag="ACGI59_003145"
     CDS             3046..3870
                     /gene="pstA"
                     /locus_tag="ACGI59_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFG9740921.1"
                     /translation="MRKFKENLLKSLVYLSALFTIVSLVVIVGFIFIKGIGNMNFNFL
                     FSNYSASGDGGILPMIITTLYTVVVSIVVATPIGILAAIYLQEYAKKGKAVTAIRFAT
                     ESLSGIPSIVYGLFGGIFFVVTLKMGYSIVAGSLTVAIIILPVIIRTTEEALKTVPQA
                     YRESSLALGATKFQTLYKVVLPSAIPGILSGVILSVGRIIGESAAILLTAGTVAKMPG
                     GIFDSARTLTVHSYLLTKESGDIATAASIGIVLIVIVLALNMLARFVAKKLNKANY"
     gene            3889..4653
                     /gene="pstB"
                     /locus_tag="ACGI59_003146"
     CDS             3889..4653
                     /gene="pstB"
                     /locus_tag="ACGI59_003146"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFG9740922.1"
                     /translation="MELIDKIKMSVKDLDLFYGDKQALKKINMDIKENKVTALIGPSG
                     CGKSTFIRTLNRMNDLIEDVTIKGNISVDGEDIYTSDDVINLRTKVGMVFQKPNPFPM
                     SIYDNVAYGPRTHGLRDKKQLDKIVEESLKGAAIWDEVKDRLKSSALGLSGGQQQRIC
                     IARAIAMRPEVILMDEPTSALDPISTLKVEELIEDLKKDYTIVIVTHNMQQAARISDE
                     TAFFLNGEVIEFSDTKTMFTTPVDKRTEDYITGRFG"
     gene            4681..5334
                     /gene="phoU"
                     /locus_tag="ACGI59_003147"
     CDS             4681..5334
                     /gene="phoU"
                     /locus_tag="ACGI59_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFG9740923.1"
                     /translation="MVNNSLDISIDTLKEQTVLMMEKCEEAIEKSVTCMIEKDLEGAR
                     KVVEDDEEINKLREEIRDKSIELIVLKQPMARDLRFIYSLGNISIELERIGDYAANIA
                     REMLKIGGEAYIKELIDIPKMAKECKSMMSQTREALILFDASIAYDVGTKDDIVDGLY
                     EQVQIDCLKIMHENSNTIDQGVKLLLIGRYLERIGDHITNVCEKIIFAIEGKMVEIG"
     gene            5492..6361
                     /locus_tag="ACGI59_003148"
     CDS             5492..6361
                     /locus_tag="ACGI59_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HFG9740924.1"
                     /translation="MKLQKMGMRTIKTGISVTLCMILGDYLVENMFYSLVACIISVQD
                     TVKGSLKTGINRVEGTILGGIIGFIFAIIKAGDPFLCGLGVMCSIYLCNLFKIKSVVV
                     ACVTFLAIHLGVGNSDPVYYSLHRVLDTSVGVLFGVLVNYYVARPNYLNSVIDEFKKI
                     EELSMLLIEDKILRKENLNVEKFEEEVEKLEEIYSKLIDELDYSLSETNIEKIENALR
                     ICREIHFHMQSIELLENKLYLKEHTYKYLKKTYNVDKLDWNIDEEKSPVFNYHLMKIM
                     IEIELLRKLNKSY"
     gene            6448..7884
                     /locus_tag="ACGI59_003149"
     CDS             6448..7884
                     /locus_tag="ACGI59_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HFG9740925.1"
                     /translation="MNTRNDNSKILEIYKMKLFGELVKAVWNDDIEDIENLPNKILNE
                     NLKDNLNNYFSGKFDKDTMVNLIRVTMGLDVTEDTDKNLKESLHEAVNLDEVKRPIIS
                     VISDACKYCDENNKEDESCKIRSKHINCNEEDVCSACGDCVSRCKLGAISDKIEFIPM
                     IKLLKDIKSPVYAIVAPAFVGQFGKDVSPGKLRTALKKIGFEDMIEVALAADMLTAKE
                     AYDYYEHMKKDEEGCFITSCCCPIWVSLIQNSFPQILENVSPSVSPMIACGRAVKILN
                     PDAKVVFIGPCMAKKKEAMLEDIKDAVDFVLTFKELQEIFDALELNPAIMDESNRVEA
                     SLSGRVYARTGGVSKAVKLSVRRIDKNIKFEEKAFEGTKECMDGLKKVLNKEIEATFI
                     EGMGCVGGCVGGPKRILSVEEGTKNVDEYSESTNMETPFDNLNVLQFLTSLGIKRVES
                     LGEKEEEKVKKIFSRDIKSNKKENKYKK"
     gene            7932..8759
                     /locus_tag="ACGI59_003150"
     CDS             7932..8759
                     /locus_tag="ACGI59_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFG9740926.1"
                     /translation="MKIKDNKAVFHVFIRIFLIAVLILTGTLLEEKINNVPVENISSK
                     GENTLELLSQSNTLGEIIYKASQEETKGPGATSEKVAYITIDDGPSKYTNALLDILKE
                     NDVKATFFMLNQNMKNHKEEVRRVLEEENSIGFHSVSHDIHKLYKSPEVTVGEFNTCN
                     NTLQEITGQSSKLIRLPYGSKPYMPEGSYNKLMANDYLIWDWNLDTQDWKSSTSQIVS
                     NILYYGRKRDELVVLIHEKEQSLNALNNIIRILKERGYTILPITENITPKNFWQENL"
     misc_binding    9055..9317
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            9393..12059
                     /locus_tag="ACGI59_003151"
     CDS             9393..12059
                     /locus_tag="ACGI59_003151"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFG9740927.1"
                     /translation="MENTNLSKTYNPKDFEARLYKKWMDEGYFKSKPNPDKKPFTIMM
                     PPPNITGQLHMGHALDHTLQDILIRWKRMDGYEAFWLPGTDHASIATEVKVVERIKKQ
                     EGKTKYEIGREEFLKRAWEWKDEFGGKISNQLKQLGDSCDWDKERFTMDEGCNEAVIE
                     FFVSLYEKGHIYRGNRIINWCPDCKTTLSDAEVEHEEHDGNFYHIKYPLKDSEDFLEI
                     ATTRPETMIGDTGIAVNPEDDRYKHLIGKTAILPLVGRELPIVADSYVDLEFGTGAVK
                     MTPAHDPNDFEVGLRHNLEQLNTMNEDGTMNEVCGKYEGMDRFECRKAIVADLKEQGY
                     LIKIKEHNHNVGTCYRCHTVVEPRLSEQWFVKMEELAKPAIDILKKGELEFVPDKFDK
                     TYLQWLENIRDWCISRQLWWGHQIPAYYCQECGEIVVARKMPDKCPKCGSTHFKQDED
                     ALDTWFSSALWPFSTLGWPNKTEALDYYYPTSVLVTGYDIIFFWVVRMAFAGMFCMNE
                     KPFDHVLVHGLVRDSQGRKMSKSLGNGIDPLEIIEQYGADALRFTLTTGNSPGNDMRF
                     YMERVEFARNFANKLWNASRFVFMNIDEDIIKNMTRESVKEDLTLADKWIISRANNIV
                     KEATNNMDKFDLGIALQKIYDFTWSEYCDWYIEMVKPRLYGEDANAKSAALYTLTYVL
                     EKILKLLHPYMPFITEEIYTHLPTVEGCIIVSEWPKYNEEDNMAEEEDMMNLLMEGIR
                     SIRNVRAEMNVPPSKKAKLIIIPSEEKIEAIELGKDYFITLASASNVEIAKDKSNVPE
                     DAVGVVIDGVEIFIPLNELVDFEKEIERLSKEKKKLEGEIKRVNGKLANQGFLAKAPE
                     SLIEEEKAKKEKFEEMIKSVEERLTNLESKIK"
BASE COUNT         4870 a         1285 c         2231 g         3828 t
ORIGIN      
        1 ttcaacagga tagtttgaaa gttgaataat tttatagata tgaaagttat ttttcttcta
       61 agaaaacaaa ctaaaacagt agaatatatg gtaaaaatta cctatagttt ttgtcctaaa
      121 aagctcctat tattaattta agctagataa atttgaagaa gtgacataaa ttgaaagaag
      181 gaatatatta tgttgaatag aaaactacca gatgcagaat taaaaattat gaaatacata
      241 tggaatactg gttataaaac tgtaatatca aaagatgttg cagatgaaat agaaaaaaca
      301 tatggttgga agcatacaac tacaattaca cttttagcta gattaaccaa aaaaggtttt
      361 ttaatatcgc agagaattgg gaaacatgta cactatacag tattagtaaa agaaagagag
      421 tatctaaaat tagaaggtaa aagaattttt ggaggtttac ataacaatcc cttgtcagaa
      481 ctaatttcaa aattgcatga tggagaagaa ataactgaag aaaaaataat agaaatagga
      541 aattggataa aaagctggaa agatgaagat taataaataa aaggtagcta ctaatataag
      601 tgctaccttt tttcttgtgg ataatattgg ggataagttg tggaaaactt atgtaaaatt
      661 tgtggataaa atattttact gacatttgac tgacataaat atcaaaactt gattttaaag
      721 tattgaaatt tcaattatta atcatataaa aatagaaaag ttattattgt aataaagaat
      781 aataaatata ttaatatcat taataattgt tataaatgtg aaatactaac aatatctaaa
      841 ctaaattatt ataaaatagc tgactgtaat ttaattgata aagcctattc ttctagactt
      901 tgccagttaa attacagtca gttttattat ataaaaaaat gcgtaatgga actattattt
      961 tatagattat atttgaaaag atagataaat tgtatatcta aatctatata ttattttttt
     1021 acctttaata tatattaaat aataaaatag ttaaataata agattaagaa tttcttaata
     1081 aatccagaag atgctttcga ttatataata ttttatattt ttatattatt aattatatat
     1141 gttgcaattg ctgcacctac tttttctggt gagtatcaga cacaatcaga tagcatcctt
     1201 cgttgtacga aacatggaca tatttgtcta gcgtttacta aaatattagc tctattttct
     1261 atatttataa taatgtttgt tatatgtatt tctatctatt tggcaatttc tgatttagct
     1321 tttggtactg aatgtttgaa aacctcattt caaatgttgt tttctgtcat tagtctagta
     1381 aatataaatt tactacaagc acagattctt atagctattg gtggtctaat ttcaatactt
     1441 gcaatggtta gtttaacatt atttttatct gcaaaatgta aggattctct tacttctatg
     1501 ctgattgctt tttctatttg cttaattcct atgggaatag gtgttagttg gattggatac
     1561 ctattaccgc cagggggaat tggttttaag attagtttat tatatcaaat gacatcattt
     1621 aatttcttac acttaggagg tatgagtttt tggacaccac acattatact gtttttctca
     1681 gtgatagaaa tacctatttt tatatttttg gctattcata cctattgcaa acatcaagta
     1741 tcgtaacaat ttgatggaaa taatagtgat ataaactaga ggatacaata attatatatg
     1801 tagtataaaa gttaaattta gttaacaaat cttaatagca gatataggta ataaattata
     1861 cataaactgt attattatct atatctgcta ttttaatata gagaaacaac agtatattgt
     1921 aaagtaaatg tcaaatatag ttttaaaagt aaaattaatg ttaaaaagta aatataattt
     1981 tacacttgct taacttaatc ctaatataga tttaacttaa aaaaagtaga ataaaagtac
     2041 aggaaatttg atgaagacga attataaaaa attaaatttt gaatacagaa cgaaaatcga
     2101 taagtatatt ttaatgggag ggtattatga tagtaaaatc tagattagta ggtttagaaa
     2161 aaaatgacaa taatggcaat aagacaaaat acgtaattga aaaagtagca aaaaatattt
     2221 ttctacttag tgctttagta gcagttgcaa gtttactttt gataatagga tttgtatttt
     2281 ataaaggatt aagaccattt atatttgaag gatattcatt tatagacttt ttaactggtg
     2341 cagattgggt tccttctgcc aataaatttg gaatatcatc tatgatagta gcatcaatat
     2401 ttgcaacact tggagcactt gttataggag tacctgttgg tatattaacc tctgtattta
     2461 tagcagaagt agcacctaaa agattagcaa aaataatgtc accagcagtt gaacttttag
     2521 caggtatacc ttcagtatta tatggtgtat ttggtcttgc agtgatagtt ccaactatac
     2581 aaagtatatt taatcttcca aaaggtcaaa gtttgttagc aatagtaata gtattagcag
     2641 taatgatgtt accaacaata atatctgtat cagaaacagc aataagagca gtaccaaaag
     2701 catacaaaga aggttcatta gcacttggtg catcaaaaat agaaacaata ttcaaagttg
     2761 tattacctgc tgcaaaatct ggagtattag ccgcagtagt gttaggagta ggaagagctt
     2821 taggtgaaac aatggcagta atacttgttg caggcaactc accagttatg ccaactgcaa
     2881 taacagatag tgtaagacca cttacaacta atatagcact tgaaatggga tacgcatttg
     2941 gtacacatca agaaatgcta tttgcaactg gagtagtatt gtttacattt atattaattt
     3001 taaacttagt tttaaataaa ctttcaaata aggcggtaaa ttaagatgag aaagttcaaa
     3061 gagaatctat tgaagtcatt agtatatcta tcagcactat ttacaatagt atcattagta
     3121 gttatagtgg gatttatatt tataaaggga attggcaaca tgaattttaa tttcttattt
     3181 agcaattatt cagcatcagg tgatggtggt atacttccaa tgataattac aacattatat
     3241 acagtagtag tttctatagt tgtagcaact cctataggaa tattggctgc tatatattta
     3301 caagaatatg ctaaaaaagg aaaagcagtt acagctataa gatttgcaac tgaaagttta
     3361 tcagggatac catctatagt atatggatta tttggaggaa ttttctttgt agttacatta
     3421 aaaatgggat attccatagt tgcaggttca cttacagttg caataataat attaccagta
     3481 ataattcgta caacagaaga agctttaaaa acagttcctc aagcttatag agaatcatca
     3541 ttggcattag gagctacaaa atttcaaact ttatataagg tagtacttcc aagtgcaata
     3601 ccaggaattt tatctggagt aatcttatca gttggtcgta taattggaga atcagctgca
     3661 atattactta cagcaggtac tgtagccaag atgccaggtg gaatatttga tagtgcaaga
     3721 acattaactg tacactcata cttactgact aaagagagtg gagatatagc aacagcagcc
     3781 agtataggaa ttgttttaat agtaattgta ttagcactaa acatgctagc aagatttgtt
     3841 gctaagaaat taaataaagc taattattaa atttaagagg tgtgaataat ggaattaatc
     3901 gataagataa aaatgagtgt aaaagatttg gatttatttt atggtgataa gcaagcatta
     3961 aagaaaataa atatggatat aaaagagaat aaggttactg ctttaatagg tccatctgga
     4021 tgcggaaaat caacttttat aagaacgtta aatagaatga atgacttaat cgaagatgta
     4081 acaattaaag gaaacatttc tgttgatgga gaggacatat atacctcaga tgatgttata
     4141 aatcttcgta ctaaagtagg aatggtattt caaaagccaa atccatttcc tatgagtata
     4201 tatgataatg tggcatatgg tcctcgtaca catggactaa gagataagaa gcaattagat
     4261 aaaattgtag aagaaagctt aaaaggtgca gctatttggg atgaggttaa agacaggctt
     4321 aaatcatctg cactaggact ttctggtggt caacaacaac gtatatgtat agcaagagca
     4381 attgctatga gaccagaagt tattcttatg gatgaaccta catctgcttt agacccaatt
     4441 tcaacattaa aagtagagga acttatagag gatttaaaaa aagactacac aatagtcata
     4501 gttacacata atatgcaaca agctgctcgt atatcagatg aaacagcttt tttcttaaat
     4561 ggagaggtta tagaatttag cgatacaaaa actatgttta ccactcctgt agataaaaga
     4621 acagaagact atataacagg tagatttggt taaaatatat aactataaag gagttagaca
     4681 atggtcaata atagcttaga tataagtatt gatactttaa aagaacaaac agtgcttatg
     4741 atggaaaaat gtgaagaagc aatagaaaag tctgtgactt gtatgataga gaaggattta
     4801 gagggtgcaa gaaaagtagt agaagatgat gaagaaataa ataaattaag agaagaaata
     4861 agagataaga gtatagagct aatagtatta aagcaaccaa tggcaaggga cttaagattt
     4921 atttattcat taggtaatat atctatagag ctggaaagaa taggagatta tgcagcaaat
     4981 atagctagag aaatgttgaa gataggtgga gaggcttata taaaagagct aatagatatt
     5041 cctaagatgg caaaagaatg taagtctatg atgtctcaga ctagagaagc tttaatctta
     5101 tttgatgcaa gtatagctta tgatgttgga actaaagatg atattgtaga tggattgtat
     5161 gaacaagtac aaattgattg tttaaaaata atgcatgaaa actcaaatac catagaccaa
     5221 ggggtaaaac ttctacttat aggaagatac cttgaaagaa ttggagacca tataacaaat
     5281 gtatgtgaga agataatttt tgcaattgag ggtaaaatgg ttgagatagg ataaaaaact
     5341 gctttaaagc agttttttta ttgtctaaat taagaaagaa ttgataaaaa aatatgatta
     5401 atgggtaaat ttaaaaaatt gtgatatact aggaacatat taaatatgag gatgcgtatt
     5461 ttatataagg tgctttttag gaggtggaac tttgaaactt caaaagatgg gtatgaggac
     5521 tataaaaaca ggaattagtg tcactttatg tatgattctt ggtgactatt tagtagagaa
     5581 tatgttttat tctctggtag catgtataat ttctgttcaa gatacagtaa aaggttcttt
     5641 aaagacggga atcaatagag tggaaggtac tattttaggt ggtataatag gattcatatt
     5701 tgccatcatt aaagctggag acccattttt atgtggtcta ggagtaatgt gctcaatata
     5761 tttatgcaat ctgtttaaaa taaagtcagt agtagttgcc tgtgtaacct ttctggctat
     5821 acatctaggt gtaggaaata gtgaccctgt gtattattct ttacataggg tgttagatac
     5881 atctgtgggt gttttgtttg gagtacttgt taattattat gttgctagac caaattatct
     5941 aaatagtgtt atagatgagt ttaaaaagat agaagaactt tcaatgttgc ttattgaaga
     6001 taaaattctt agaaaagaaa atttaaatgt agagaaattt gaagaagaag tagaaaaatt
     6061 agaagaaata tattcaaaac ttattgatga gttggattat agtttgagtg aaactaatat
     6121 agaaaaaatt gagaatgcct taaggatatg tagagaaata cattttcata tgcagtctat
     6181 agagttactt gaaaataaac tttatttgaa agagcataca tataaatacc ttaagaaaac
     6241 atataatgtg gataagctag actggaatat agatgaagaa aaaagccctg tattcaacta
     6301 tcatctaatg aaaataatga ttgaaataga gttgttgaga aaactaaata aatcatatta
     6361 ataatagtag ttaaataata aataaaaaat agtatctttg taaatattaa acatatataa
     6421 gtttaattta tggaggtact attttttatg aatactagaa atgacaattc taaaatacta
     6481 gaaatatata aaatgaagct ttttggcgaa cttgtaaaag cagtgtggaa tgatgacata
     6541 gaggatattg aaaacttacc taataaaatt ttaaatgaaa atttaaagga taatttaaat
     6601 aattatttta gtggaaaatt tgataaagat actatggtaa atttaattag ggtaactatg
     6661 ggattggatg tgacagaaga tacagataaa aatttaaaag agtcactaca tgaagctgta
     6721 aatttagatg aagtcaaaag accaataata tctgtaatat cagatgcttg caaatattgt
     6781 gatgaaaata ataaggaaga tgagtcatgt aagataagaa gtaagcatat taattgtaat
     6841 gaggaagatg tatgtagtgc ttgtggtgat tgtgtatcaa gatgtaagtt gggtgcaatt
     6901 tcagataaaa tagaatttat accaatgata aaattgttaa aagatataaa gagccctgta
     6961 tatgcaatag ttgcaccagc ttttgtagga cagtttggga aagatgtaag ccctggtaaa
     7021 ttaagaactg cattaaaaaa gataggattt gaagatatga tagaagttgc tttggcagca
     7081 gatatgctta ctgcaaaaga agcatatgat tattatgagc atatgaaaaa ggacgaagaa
     7141 ggatgcttta taactagttg ttgctgtcct atttgggtta gtttgataca aaatagtttt
     7201 cctcaaatat tggagaatgt atcaccatcc gtttctccta tgattgcttg tgggagagct
     7261 gtaaaaattt taaatccaga tgctaaagtt gtattcatag gtccttgtat ggcaaaaaag
     7321 aaagaggcaa tgttagaaga tataaaagat gcagttgatt ttgtgcttac atttaaagag
     7381 ttgcaagaaa tatttgatgc attagagtta aatccagcaa taatggatga gagtaataga
     7441 gtagaggcgt ccctttcagg tagagtttat gctagaacag gaggtgtaag taaagcagtt
     7501 aagttatcag ttagaagaat tgacaaaaat ataaaatttg aagaaaaggc atttgaagga
     7561 acaaaagagt gcatggacgg tctaaagaaa gttctaaata aagaaataga agctacattt
     7621 atagaaggaa tggggtgtgt aggaggctgt gtaggaggtc cgaagcgtat tttaagtgtg
     7681 gaagaaggta ctaagaatgt tgatgaatat agtgaaagta cgaatatgga aactccattt
     7741 gataatttaa atgtattaca atttttaact tctttgggaa tcaaaagagt agaatcttta
     7801 ggagaaaaag aagaagaaaa ggttaaaaaa atatttagta gagatataaa aagtaataag
     7861 aaagagaata aatataaaaa ataacttttt aagtcttaaa cttatgcttt tataaaaggg
     7921 agatatgggg gatgaaaatt aaagataata aggctgtttt tcatgtattt ataaggatat
     7981 ttcttatagc tgttttaatt ttaactggaa cattattgga ggaaaaaatt aataatgttc
     8041 cagttgaaaa tataagttca aaaggtgaaa atacactgga actgctttct caaagtaata
     8101 ctttaggaga aatcatatac aaagcatctc aagaagaaac aaaaggtcca ggggctactt
     8161 ctgaaaaagt agcatacata acaatagatg atggtccatc aaaatataca aatgcactac
     8221 ttgatatttt aaaagaaaat gatgtcaaag cgacattttt catgcttaat cagaatatga
     8281 aaaaccacaa ggaagaagta aggagagtct tggaagaaga aaactcaata ggttttcata
     8341 gtgtaagtca tgatatacat aagttatata aaagcccaga agtaacagtt ggggaattta
     8401 atacatgcaa taatactcta caagaaataa ctggtcagag ttcaaaatta ataagattac
     8461 catacggaag taaaccatat atgccagaag gttcatataa taaacttatg gcaaatgatt
     8521 atttaatttg ggactggaat ttggatacac aggattggaa gtcttctaca agtcagatag
     8581 taagtaatat attatattat ggaagaaaaa gagatgaatt agtagtatta attcatgaga
     8641 aagagcaaag tttaaatgct ttaaataata ttataagaat cttaaaagag agaggatata
     8701 caatattgcc aattacagaa aatataacac ctaaaaattt ctggcaagaa aacttataat
     8761 aaatttaaat atagtaaaag ataaagtaaa ttatttataa aataaaacat ataatataaa
     8821 aaatacgaat aagaggtata ctgtaaggat aatataaaat taatgagaaa gcattaaaat
     8881 taaaataaaa taatcttgca attattgaca ttataagaca gaataactgt aaaaacatat
     8941 tttaaaatat aacaactata taaaagagaa tagacataat taagaaaatt cagaaaaaat
     9001 aaaataaaaa taattgacaa agagaataat atttgtatat aataatatgt aatattaaag
     9061 actgagaaga agaggagtaa gtaaatacta ctaaaagaga ggaaaattca ctggctgtaa
     9121 gattttctaa gtataagatt tactgaatgt agctttggag tttctaaact gaactataag
     9181 taagtttaga cggttagaac cgttaaattc tttgagagtc aaagttgata aaataacatt
     9241 aaccaataat ttaatgatat gttaatgtat tttaagcttt ggaattaagg tggtaacgcg
     9301 agcttttcgt cctttttaaa gaggatgaag agctcttttt tatttctaaa gatatttaaa
     9361 aaatttattt ataaaataca ggaggcagga caatggaaaa tacgaattta tcaaaaactt
     9421 ataacccaaa agattttgag gctaggctat ataaaaaatg gatggatgaa gggtatttta
     9481 aatctaaacc aaatccagat aaaaaaccat ttacaataat gatgccacca ccaaatataa
     9541 cagggcaatt gcatatggga cacgctcttg accatacact tcaagatata ttaatcagat
     9601 ggaaacgtat ggatggatat gaagcttttt ggcttccagg aactgaccat gcttctatag
     9661 caacagaagt taaagttgtt gaaagaataa aaaaacaaga aggcaagaca aagtatgaaa
     9721 taggtagaga agaattttta aagagagcct gggaatggaa agatgaattt ggaggaaaaa
     9781 tatctaatca gcttaaacaa ctaggtgatt cttgtgattg ggataaagaa agatttacta
     9841 tggatgaagg gtgtaatgaa gcagttatag aattttttgt aagcctatat gaaaaaggtc
     9901 acatatacag aggaaataga ataataaact ggtgcccaga ttgcaaaact actttatcag
     9961 atgcagaagt tgaacatgaa gaacacgacg gaaactttta tcatataaag tacccactaa
    10021 aagatagtga agacttttta gaaatagcta caactagacc tgagacaatg ataggagata
    10081 ctggtatagc agtaaatcca gaagatgata gatataaaca tttaatagga aaaacagcaa
    10141 tacttccatt agtaggaaga gaacttccaa tagttgcaga tagttatgtt gatttagagt
    10201 ttggaactgg tgcagttaag atgactccag cacatgaccc taatgatttt gaagtaggat
    10261 taagacataa tcttgagcag ttaaatacta tgaatgaaga tggaactatg aatgaagttt
    10321 gtggaaaata tgaaggtatg gatagatttg agtgtagaaa agctatcgta gcagacttaa
    10381 aagaacaagg ttacctaatt aagataaaag agcataatca taatgtagga acttgttata
    10441 gatgccatac agtagttgag cctagattat cagagcaatg gtttgttaag atggaagaac
    10501 ttgcaaaacc agctatagat attcttaaaa aaggagaatt agaatttgtt ccagataaat
    10561 ttgataaaac ttatttacaa tggctagaaa atataagaga ttggtgtata tcaagacaat
    10621 tatggtgggg tcatcaaata ccagcttatt attgtcaaga gtgtggagaa atagtagttg
    10681 ctagaaaaat gccagataag tgtcctaaat gtggaagcac tcattttaaa caagatgaag
    10741 atgccttaga tacttggttc agttctgcat tatggccttt ctctactcta ggatggccaa
    10801 ataagactga agctttagat tattattatc caacaagtgt acttgttaca ggatacgata
    10861 taatattctt ctgggtagtt agaatggcat ttgcaggtat gttctgtatg aatgaaaagc
    10921 catttgacca tgttttggtt catggattag taagagattc tcaaggtaga aaaatgagta
    10981 aatctttagg aaatggtatc gacccattgg agataatcga gcaatatgga gctgatgccc
    11041 taagatttac attaacaact ggaaattccc caggaaatga tatgagattc tatatggaaa
    11101 gagtagaatt tgctagaaac tttgcaaata agttgtggaa tgcatcaaga tttgtattta
    11161 tgaatattga tgaagatata attaaaaaca tgactagaga aagtgttaaa gaggacttaa
    11221 ctctagctga taaatggata atatcaagag caaataatat tgtaaaagaa gcaactaata
    11281 acatggataa gtttgattta ggaatagcac ttcaaaagat atacgatttt acttggtcag
    11341 aatactgtga ctggtatatt gaaatggtta aacctagatt atatggagaa gatgcaaatg
    11401 ctaaatctgc agcattatat acattaactt atgttcttga aaaaatatta aaattattac
    11461 atccatacat gccatttata acagaggaaa tatatactca tcttcctact gttgaaggat
    11521 gtataatagt atctgaatgg ccaaaatata atgaagaaga taacatggct gaagaagaag
    11581 atatgatgaa cttattgatg gaaggtataa gaagtataag aaatgtgaga gcagaaatga
    11641 atgtaccacc ttctaagaaa gctaaattga taataatccc aagtgaagaa aaaatagaag
    11701 ctattgagct tggtaaagac tattttataa ctttagcatc tgcttcaaat gtagagatag
    11761 caaaagataa atctaatgtt ccagaagatg cagttggagt agttattgat ggtgttgaaa
    11821 tatttatacc tcttaatgaa ttagttgatt ttgaaaaaga aattgagaga ttatctaaag
    11881 aaaagaaaaa gttagaaggc gaaataaaga gagttaatgg aaagcttgca aatcaaggtt
    11941 tcttagcaaa agctcctgaa agtttaattg aagaagaaaa agcaaaaaaa gaaaaatttg
    12001 aagagatgat taaatctgtt gaggaaagat taacaaattt agaatctaaa ataaaataaa
    12061 tataagaatt agtaaaaaca tacaaaaagt atatattcta tactttttgt atgtttttct
    12121 ttatatatat acaaatatga tgttataatt ctttacagat gtgttatatt tttattttat
    12181 gttgtgaata tatgaatttt atgtcttaaa attt
//
LOCUS       DAYAPA010000126        12088 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.128, whole genome shotgun sequence.
ACCESSION   DAYAPA010000126 DAYAPA010000000
VERSION     DAYAPA010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12088
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.128"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            <1..410
                     /locus_tag="ACGI59_003152"
     CDS             <1..410
                     /locus_tag="ACGI59_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4004 family protein"
                     /protein_id="HFG9740928.1"
                     /translation="IANMFMKKDINKEFDIDFILSKEVICNYTKEIFQNLYKKEQSIG
                     KKELLILSIIEKFLVKSIITFEELKLIVNIIEENFTSIYNESGKIYLFRKFGVPFVVG
                     CLDYKKVKFEKDVVKIIEVDLTKEINDISIKLS"
     gene            complement(710..1297)
                     /gene="rbr"
                     /locus_tag="ACGI59_003153"
     CDS             complement(710..1297)
                     /gene="rbr"
                     /locus_tag="ACGI59_003153"
                     /EC_number="1.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016692 - NADH peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin"
                     /protein_id="HFG9740929.1"
                     /translation="MNLKDSKTKENLLRAFAGESQARNRYNISASVAKKEGLYIIESL
                     FNYTADQEKSHAKQFFSKLKEFAGENISISGAYPVDLYDKTAQHLKAAQHNELQEWDD
                     VYKNFGKTAREEGFESIAKLFENIAEIEKVHGDRFGRYATDLEEGTLFRRNEDIQWIC
                     THCGHIHIGKEAPKACPVCLHPQGYFLDFKNSLFE"
     gene            complement(1383..1820)
                     /locus_tag="ACGI59_003154"
     CDS             complement(1383..1820)
                     /locus_tag="ACGI59_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFG9740930.1"
                     /translation="MEDKYIVHFISKTKASMIKFIENKLSKNGLGELIPTHGNILTAL
                     YENNGILTMKEIAAKIGKDKSTVTVLVNKLINLGYLERQKCTNDKRITYIKLTKKALL
                     IEDTFNSISTQVKETAYHNITENEKQEFLRILKKINDNFKNAD"
     gene            1924..2922
                     /locus_tag="ACGI59_003155"
     CDS             1924..2922
                     /locus_tag="ACGI59_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdH/YhfP family quinone oxidoreductase"
                     /protein_id="HFG9740931.1"
                     /translation="MNKKFKSLYITEEEGNFRRNIVEREVDTLPEGEVLIKVKYSSLN
                     YKDALSANGNKGVTRKYPHTPGIDASGIVESSSCDDFKIGEEVLVTGYDLGMNTSGGF
                     SEYIRVPSSWVVKLPKNLSLKEAMDYGTAGFTAALSVYKFIDSVDKNMGDVLVTGGTG
                     GVAVIATKILIKLGYSVVVSTGKLEEQKEVLLNLGVKDVIHRSEVDDNSGRPLLRPRW
                     AGVIDTVGGNTLATAIKTTNYCGAVTTCGNAGGVDFTSSVYPFILKGITLYGIDSVDC
                     PMNLRLKIWDLLSNEWKLDDLDDMVNVVSLEELNDSIDSIMAGKHVGRTVVDLEKI"
     gene            complement(3042..3968)
                     /locus_tag="ACGI59_003156"
     CDS             complement(3042..3968)
                     /locus_tag="ACGI59_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HFG9740932.1"
                     /translation="MSKENKNVRIAVVQASPVIMDLEKTVEKALGLIKEAGRKGANIV
                     VFPEAFIPAYPRGLSFGFVVGSRTMEGREDWKRYYDNSVPVPSATTDLLGKAAQEAGV
                     YLSMGITERDGNDINCTLYCTNLFFSPEGKLIGKHRKLKPTGTERCIWGEGDGSTLTV
                     VDTPYGKMGSLICWENYMPLARTALYAKGVKLYIAPTADSREEWQATMKHIALEGRCF
                     VIGCNQYVEKNMYPTDLNYYKELDAEPEIMCPGGSCIVDPFGKYVAGPIFNKEEMLIA
                     DLDLEKIVLSRLDFDSEGHYSRPDVFELIVHE"
     gene            4353..5957
                     /locus_tag="ACGI59_003157"
     CDS             4353..5957
                     /locus_tag="ACGI59_003157"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HFG9740933.1"
                     /translation="MKEKIYYNGNIITMEDSICGDAILIKDKIIKKIGTKEEVFALKN
                     KDTEIIDLQGKTLMPSFIDSHSHLIAFATTLKLVPLEDATSFKDIVKKIQDFKESNNI
                     KKGDWIIGFSYDNNFLEENKHPDKSVLDSASSENPILISHASGHMGVANTLGLKQLGV
                     TNETRDPEGGHIGRVEGSEEPNGYLEENAFFNVASKIKQPSSNEIFNSIEKAQNIYLS
                     YGITTAQEGLMEENQFNILKAMANQNKLKMDVVGYVNLKKSKSVADNNREFIKKYINR
                     FKIGGYKIFLDGSPQGKTAWLSRPYENSDDGYCGYPIYKDEEVEKFIDISLKEKMQLL
                     THCNGDAAADQLIDAFEKVLNLKEQSSENHIRPVMIHAQTVREDQIDDMKVINMIPSY
                     FVAHTYYWGDIHIKNLGEDRAFKISPLKTTIEKGLIYTLHQDTPVIAPNMLETVWCAV
                     NRITKKGIQIGENEKISPLDALKGVTINAAYQYFEEDKKGSIKEGKLANLIILDENPL
                     TIDPMKIKDIKVLQTIREGEVLYSLK"
     gene            6773..8044
                     /gene="serS"
                     /locus_tag="ACGI59_003158"
     CDS             6773..8044
                     /gene="serS"
                     /locus_tag="ACGI59_003158"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006441208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFG9740934.1"
                     /translation="MLDIKRIRENLDCIKKAMERRGEKDFNLDEVVKLDDERRKILQE
                     VEVMKNELNTASKNIPNLIKEGKDVENEKIKLKELSDKIKVIDQNLKEVEDKMEYLLM
                     RIPNVPHPEVPQGETDEDNVEVRTWGKTTTFDFESKAHWEIGTELGILDFETASKITG
                     SRFTLYKGLGARLERALLNFYLDTNTKVNGYTEVIPPFMANRNSFLGTGQLPKFEEDM
                     FKIEGMDYFMIPTSEVPLTNIHANEILKFEQLPINYTAYTPCFRSEAGSAGRDTRGLV
                     RQHQFNKVEMVKIVAPEESYNELEKLTNNAETMLQLLNLPYRVVKICTGDLGFTASFK
                     YDVEVWMPSYNRYVEISSCSNCEDFQARRAGIRFKRDKDSKAEYVHTLNGSGLAIGRS
                     VAAILENYQQEDGSVVVPEVLRPYMGVSVIK"
     gene            8322..9827
                     /locus_tag="ACGI59_003159"
     CDS             8322..9827
                     /locus_tag="ACGI59_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFG9740935.1"
                     /translation="MKIQNTEWGYIEWKHTYDENNPKQAMNIYIAVTMPGKKHFNHVH
                     YGQEQMIYILEGEGLYIINGVWKPFYQGMIFYIESGSTHETINTGDREIKELIVSNNV
                     DDVGESEVIDINPNNYLKKTLINYSESTLNLYAAVESIRGQFIDPFKIPLIIYDDSWN
                     IVLKNPYFPLFCFEKCNPMKFPQNCDCMNQKSSNQFVCEYGITIYNIPILYKSNSIGV
                     IRGGYVLLSDLNLDTEHNNLYDIPEGAARSIKRLLKQISKNIINFCSFNDIRKDLQEK
                     EKTIARTYHYGEQLEMNLKVAQDMVTNLRINHHFLFNTLNSMASIALDDGSYDLYSAI
                     IDLSRMFRYTMRSDLRFVELESEILYIKNYLNLQKLRYGDALKVKYLIPEKLYNLSVP
                     FNFIQPIVENAFTHGFRDIDTEKRIEIIARLDSQYAIIEIHNNGTILDGNNIDKIKAG
                     IRSNNGHGLSLIYTKFTSAYGNNFDMDIKSSDNEGTYIMIKIPIENYKEYV"
     gene            9941..10639
                     /locus_tag="ACGI59_003160"
     CDS             9941..10639
                     /locus_tag="ACGI59_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFG9740936.1"
                     /translation="MRRALIIDDEEAVLKIITHFIEMKDMPIKIVGKATSGDEAVDKI
                     IGLEPDIVFIDIQMPIYNGLEVIEKTSHLSKKINFVVITAFNYFEYAQKALRLNVKDI
                     LLKPLDMKEFTKSVEKIIGYQYTNNDLLNEILEYINLNYSNDLKLNDCARLFLTNPSN
                     ISRIFKSNLNTTFISYLNHIRIEKSIELLENTTKSINEIAEIVGYNSLNNFYKNFKIE
                     KGMTPKVYKLNTKN"
     gene            10924..12012
                     /locus_tag="ACGI59_003161"
     CDS             10924..12012
                     /locus_tag="ACGI59_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9740937.1"
                     /translation="MSDKVNMKNIICIAGAYLATAIGSGFATGQEILQFFASQGYMGL
                     IGALISMVLFSFLGAEVITRGRELKLKEPTRIYTFYCGKILGKFYEWFSPIFLFGILI
                     VMISGAGSTLTEYYGINPYLGRVLMAVATLITVSMGLEKLVSILGRIGPVIAVFTIAV
                     GLISLFKHFDGLSMVGEAMKNIEVTKASESPIVSGVIYNTLNIIVMIVFLTGMGASIE
                     SKKDAFWGGLLGGIVFMTAGIVMYLAMISDIGNLYMKEIPSLYLADNISPIVGVCFSV
                     ALILGIYTTAVPLLWSVTNRIVEDEHPKFKLVTTVIAILACIGGFLPFGKLVNILYPY
                     TGYMGLLILVCMVYKAITCRKKSTQSQE"
BASE COUNT         4689 a         1354 c         1921 g         4124 t
ORIGIN      
        1 atattgcaaa tatgtttatg aaaaaggata ttaataaaga atttgacatt gattttattc
       61 tcagtaaaga agttatatgt aattatacca aagaaatatt tcaaaattta tataaaaaag
      121 aacaaagcat aggtaaaaaa gaactgctta tattgagcat aatagagaag tttttagtta
      181 aatctataat aacttttgaa gagttaaaat tgatagtgaa tataatagaa gaaaatttta
      241 catcaatata caatgaaagt ggaaagattt atttgtttag aaagtttgga gttccattcg
      301 tagtaggatg tctggattat aaaaaagtaa agtttgaaaa agatgtagtt aagattattg
      361 aggttgactt gacaaaagaa ataaatgata taagtataaa gttatcatag tgtattatct
      421 tatataatgt attataataa tctacttaaa atacacttta tttaaatatt tagtttaaag
      481 atgagatttt atgtgagtgt gtaaaagtta aagatagatt taagtaggtt atagggatta
      541 gtaaacaata aaatgaacca tgatgaacta ctataaattg tatttaaact acattttcta
      601 cttatagtaa tatcaatggt tcattttatt gttattaaga taatacagta tttataaaaa
      661 tacttgatat aaatatagta aattgtttaa atagaattat tgccttaatt tattcaaata
      721 atgagttttt aaaatctaag aaatatcctt gtggatgaag acatacagga caagctttag
      781 gagcttcttt acctatatgt atatggccac aatgtgtaca tatccattgt atatcttcat
      841 ttcttctgaa taaagtacct tcttctaaat cagtagcata tctaccaaat ctatcaccat
      901 gaactttttc tatttcagct atattttcaa ataacttagc tatagattca aatccttctt
      961 cacgtgctgt ttttccaaag tttttatata catcatccca ctcttgaagc tcattatgtt
     1021 gtgcagcttt aaggtgttgt gctgtcttgt catataaatc tacagggtat gctcctgata
     1081 tacttatatt ttcaccagca aattctttta atttagaaaa gaattgtttt gcatgtgatt
     1141 tttcttggtc agcagtgtaa ttaaataaag actctattat atataatcct tctttttttg
     1201 ctacagatgc agaaatattg tatctatttc tagcttgact ctcacctgca aaagctctta
     1261 ataaattttc tttagtttta ctatctttta aattcatatt ctacctctct atcacaataa
     1321 atttttacaa tatttgattc tttttattta ttattccaat aggagatata attatgctca
     1381 atttagtcag catttttaaa attatcatta atttttttta aaattctaag aaattcttgt
     1441 ttttcatttt ctgtaatatt atggtatgcc gtctctttaa cttgtgttga gatgctatta
     1501 aaagtatctt ctataagcag tgcctttttt gtaagcttta tatacgtgat acgtttgtca
     1561 tttgtacatt tttgtctttc taaatatcct aggtttataa gtttattaac caacactgta
     1621 acagtggatt tatcttttcc tattttagct gcaatttctt tcattgtaag tattccatta
     1681 ttttcataaa gtgcagttaa aatatttcca tgagtaggta ttaactcacc taaaccattt
     1741 ttactgagtt tattttcaat gaatttaatc atactagcct ttgttttact tatgaaatgt
     1801 acaatatatt tatcttccat aaattattct ccaatcttta atctatttaa attagtttga
     1861 cataaaacta tttataaact ataattagtt taacataaaa ctaaatgtat tgggaggaaa
     1921 tttatgaata aaaaatttaa atcactatat attacagaag aagaaggaaa ttttagaaga
     1981 aatatagttg aaagagaagt ggatacattg ccagaaggcg aagttctaat aaaagtaaaa
     2041 tattcttctc taaattacaa ggatgcactt tctgcaaatg gtaataaggg tgttacgaga
     2101 aagtatcctc atacaccagg aattgatgca tctggtatag tagaatctag tagttgtgat
     2161 gattttaaaa ttggagaaga agttttagta acaggttatg acttgggaat gaatacttct
     2221 ggtggttttt cagaatacat aagagttcct tctagttggg tagttaaact tcctaaaaat
     2281 ctttcactaa aagaagcaat ggattatggt acagcagggt ttacagcagc tttatctgtg
     2341 tataaattta tagattctgt agacaagaat atgggagatg tattagtcac tggtggtact
     2401 ggaggagttg ctgtaattgc aactaaaata ttgattaaat taggttattc agttgtagtt
     2461 tcaacaggaa aattagaaga acaaaaagaa gtattactaa acttaggtgt taaagatgtt
     2521 atacataggt cagaagtgga tgataactca ggaagacctc tgcttagacc aagatgggct
     2581 ggagtaatag atacagtagg aggaaatact ctagcaacag caataaagac aactaattat
     2641 tgtggagcag taacaacttg tggtaatgca ggaggagttg attttacaag ttcagtatat
     2701 ccatttatct taaagggaat tactttatat ggtatagatt ctgtggattg cccaatgaac
     2761 ttaagattaa aaatatggga tttattatca aatgaatgga aattagatga tttagatgat
     2821 atggtaaatg tagtatcatt ggaagagtta aatgatagta tagattcaat aatggcagga
     2881 aaacatgttg gaagaacagt agttgattta gaaaaaatat aggtgaataa atttataatt
     2941 aggattatag gttttaagca aaaatgagat acttgaaatt tacttgtgag tatctcattt
     3001 ttttatgtaa aaagatttat gtagatgttt gtgtaaaaag attattcatg aacaattaat
     3061 tcaaatacat ctggtcttga atagtgacct tctgaatcaa aatctagtct actaagtact
     3121 attttttcta ggtctaaatc agctattagc atttcttctt tattgaaaat aggacctgct
     3181 acatattttc caaaagggtc aactatacaa cttcctcctg gacacataat ttctggttct
     3241 gcatctagct ctttatagta gttaaggtca gttggataca tatttttttc aacatattga
     3301 ttgcatccaa ttacaaaaca tcttccttct aaagcaatat gcttcatagt tgcttgccac
     3361 tcttctcttg aatctgcagt aggggctatg taaagtttta cacccttagc ataaagagct
     3421 gttctagcta atggcatata attttcccaa caaatcaacg aacccatttt tccatatgga
     3481 gtatcaacta ctgtaagagt acttccatct ccttctcccc aaatacatct ttctgttcct
     3541 gtaggtttaa gttttctgtg tttaccaatt agcttacctt ctggagagaa aaataagttt
     3601 gtgcaataaa gggtacaatt tatatcatta ccatctcttt ctgttatacc cattgataga
     3661 tatactcctg cttcttgtgc tgcttttcct aataaatctg ttgtagcact aggaactggt
     3721 acagaattat catagtatct tttccaatct tctctacctt ccattgttct acttccaaca
     3781 acaaatccaa atgaaagacc tcttggatat gcaggtatga atgcttcagg gaaaactacg
     3841 atatttgccc cttttctacc agcttcttta attaatccta atgctttttc aacagttttt
     3901 tctaaatcca ttatgactgg tgacgcctgg acaacagcaa ttcttacatt tttattttcc
     3961 ttgctcataa aaacctccta aaaaatattt aaattttttg cattgaatag attacatgag
     4021 atgttatttg agaactaaga tacttatatt gtgaataata atgtaactgt atatttaaaa
     4081 taatacatca tgatttaaaa aattacaatt gtacagatat aattgagttt agatatttaa
     4141 atttaacaac ttataatatt ttttcaatta gtataaagcc tattaatttg tcttatttac
     4201 aatgaatagt atttacttct tggcaaacaa taattaaaaa taaaactttt atatgattaa
     4261 actctaaaga aaaagtttat aaagaagata atataattaa gcagaaaata atgaaaaaaa
     4321 tttattaaag tagacaacgt aaggagaatt ttatgaaaga aaaaatttat tacaatggaa
     4381 atatcatcac aatggaagat tcaatatgtg gagatgctat tcttataaaa gacaaaataa
     4441 taaaaaagat aggaacaaaa gaagaagtat ttgctttgaa aaataaagat acagaaatta
     4501 tagatttaca aggtaagaca ttgatgccat cttttataga ttcacatagt cacttgatag
     4561 cttttgctac tactctaaag ctagttcctt tagaagatgc aaccagtttt aaagacatag
     4621 taaaaaaaat acaggatttc aaagagtcga ataatataaa aaaaggagac tggataattg
     4681 gattttcata tgataataat tttttagaag aaaataagca tccagataag agtgtactcg
     4741 atagtgcatc ttcagaaaat ccaatactaa tttcacatgc ttctggtcat atgggagttg
     4801 caaatacatt ggggttaaaa cagcttggtg ttaccaatga aacacgtgac ccagaaggtg
     4861 gacatatagg tagagtagaa ggcagtgaag aaccaaatgg atatttagaa gaaaatgcat
     4921 tttttaatgt agcatctaaa ataaaacaac catcatcaaa tgagattttt aattcaattg
     4981 aaaaagcaca gaacatatat ctaagctatg gtattacaac agctcaagaa ggtttaatgg
     5041 aagaaaatca atttaacatt ttaaaagcaa tggcaaatca aaataaatta aaaatggatg
     5101 ttgtaggata tgtaaatttg aaaaaaagta agtctgtagc agataataat agggagttta
     5161 taaaaaaata tataaataga tttaaaatag gaggttataa gattttctta gatggttctc
     5221 cacaaggtaa gacagcatgg ttgagtagac catatgaaaa ttcagatgat ggttattgtg
     5281 gatatcctat atacaaagat gaagaagtag aaaagtttat agatatttca ctaaaagaaa
     5341 agatgcaact cttaactcat tgtaatggag atgcagcagc agaccagttg atagatgcgt
     5401 ttgagaaagt tttaaattta aaagagcaaa gtagtgaaaa tcatataaga ccagttatga
     5461 tacatgctca aacagtaaga gaagaccaga ttgatgatat gaaagtaata aatatgatac
     5521 cttcatactt tgtagctcac acatattatt ggggtgatat tcatatcaaa aaccttggag
     5581 aagatagagc gtttaagatt agcccactta aaacaactat agaaaaggga ttaatttaca
     5641 ctcttcatca ggatacacca gtaatagcac ctaatatgct ggaaacagta tggtgtgctg
     5701 tcaatagaat tacaaaaaaa ggtatacaaa taggagaaaa tgaaaagata agcccattag
     5761 atgctttaaa gggtgttact ataaatgcag catatcaata ttttgaagaa gataaaaaag
     5821 gttcaattaa agaagggaaa ttagctaatt tgataatctt agatgaaaac ccacttacaa
     5881 tcgaccctat gaaaataaaa gatattaaag tattgcagac tataagagaa ggagaagtat
     5941 tgtattcttt gaagtagatg aaagttagat attttataat ctataaaaaa tagttttata
     6001 taataagtta ggtagaatta aataagatta cagttaaatc aacaattata tttttattaa
     6061 attttaatta ttgtattgaa gatttataag tataatttta ttgattctac ctttttaaat
     6121 aactgtcgat ttattaaagt tatctatgta ctatattaaa caattaagta gttattaatt
     6181 gaacttatta gtttattaaa aaactatgta tattactttg cagtaagact ttggaactaa
     6241 atctataata gcaaatttaa atttattaaa tagattacct tataacattt catataaaat
     6301 atattgtgaa tctgagaaaa ttactatttt tctttgagta ttttttgtaa gcagttctaa
     6361 aagattcaat ttcataccta ttacttgaat cattcattaa aataattcta atttaaaaaa
     6421 taattctatc caaataatag atatatagga ctatgttata atataaaaaa atttatataa
     6481 aagttaataa ctatacaaga ttgataaaga aaaagtcaaa atagataatt gattagcaaa
     6541 atagatataa ttataatgaa gttaaatcta gcaatattct cctaaaatta aattacattt
     6601 atttaattaa atataatttg acaatggaca aaaaagtgag taaaatataa ataaaataaa
     6661 aataacaggt tcggataaag aaaaaggttt cttaacctca agcataaagt atattatcta
     6721 agttatttga gattaagaga taaatccaat cttaggtact gaggaggaaa aaatgcttga
     6781 tataaaaaga ataagagaaa atttagattg tataaaaaaa gcaatggaaa gaaggggcga
     6841 aaaagacttt aaccttgatg aagtagttaa gttagatgat gaaagaagaa agattcttca
     6901 agaagttgaa gttatgaaaa atgaattaaa tacagcatct aaaaatatac caaatcttat
     6961 aaaagaaggt aaagatgtag aaaatgaaaa gattaaacta aaagagttat ctgataaaat
     7021 taaggtaata gaccaaaatc ttaaagaagt agaagataag atggaatacc tattaatgag
     7081 aataccaaat gtaccacatc cagaagtacc acaaggtgaa actgatgaag ataatgttga
     7141 agtaagaact tggggaaaga ctactacctt tgattttgaa tctaaggctc attgggaaat
     7201 tggaacagaa ttaggtatac ttgattttga aacagctagt aaaataactg gttcaagatt
     7261 tactctttac aaaggattag gtgctagatt agagagagca ttattaaatt tttacttaga
     7321 cacgaataca aaagtaaatg gatatacaga agttattcca ccatttatgg caaatagaaa
     7381 tagtttttta ggtacaggac agttacctaa gtttgaagaa gatatgttta aaatagaagg
     7441 tatggactat tttatgattc caacatcaga agttccttta actaatatac atgctaatga
     7501 aatacttaaa tttgaacaat taccgataaa ttatacagct tatactccat gttttaggtc
     7561 agaagctggt tctgctggaa gagatacaag aggtttagta agacaacatc agtttaacaa
     7621 agtagaaatg gttaaaatag ttgcaccaga agaatcttac aatgagttag aaaagcttac
     7681 taacaatgcg gaaactatgc tacaattatt gaatctgcct tatagggtag taaagatatg
     7741 tacaggtgat ttaggattta cggcttcttt taaatatgat gtagaagttt ggatgccaag
     7801 ttacaatagg tatgttgaga tttcatcttg ttcaaactgc gaagatttcc aagctagaag
     7861 agcaggaata agatttaaaa gagataaaga ttctaaagct gaatatgtac acacattaaa
     7921 tggctctggg ttagctattg gaagaagtgt tgcagctata ttagaaaatt atcaacaaga
     7981 agatggttct gtggttgttc ctgaagtttt aagaccatat atgggagttt ctgttattaa
     8041 gtaagagtta aattatgtat ttttaagcaa tacaataata tagaggtaat aaaaaataat
     8101 taatataaaa gtgatacaat aatatagaag taataaaaaa atagttaata taaaagtgat
     8161 acaataatat agaagtaata aaaaaaaata attaatataa aaatgtgtac aaactaatga
     8221 atattgatat agtaagctat catatgaaag agtatgtaca catttttttt actaattttt
     8281 cacaatatga acataaataa tttacaggaa aggaatcttt tatgaaaatt caaaatactg
     8341 agtggggata tattgaatgg aaacatactt atgatgaaaa taatccaaaa caagctatga
     8401 atatatacat tgcagtaact atgcctggta agaaacattt taatcatgtt cattatggtc
     8461 aagagcagat gatatatatt ttagagggag agggtttgta tattataaat ggagtttgga
     8521 agccatttta tcaaggtatg attttttata tagaatctgg tagcacacat gaaactataa
     8581 atacaggtga tagagaaata aaagaactta ttgtttcaaa taatgtagat gatgtaggag
     8641 agagtgaagt tatagatatt aatcctaata attatttaaa gaagaccttg ataaattata
     8701 gtgaaagtac attaaatttg tatgcagcag tagaatcaat tcgtggtcaa tttatagacc
     8761 cgtttaaaat tccattaatt atatatgatg attcatggaa tatagtttta aaaaatcctt
     8821 attttccact tttttgtttt gaaaaatgta atcctatgaa atttcctcaa aattgtgatt
     8881 gtatgaatca aaaaagtagc aatcaatttg tatgtgaata tgggataact atatataata
     8941 taccaatact gtacaaaagc aatagcatag gtgttataag aggagggtat gtactattat
     9001 ctgatttgaa tttagataca gagcataata atttatatga tattccagag ggagctgcta
     9061 gaagtataaa acgcctcttg aaacaaatat ctaaaaatat aataaatttt tgctctttta
     9121 atgatataag aaaggattta caagaaaaag agaagactat agcaagaaca tatcattatg
     9181 gagagcaact tgaaatgaat ttaaaagtag ctcaagatat ggttactaat ttaagaataa
     9241 atcatcactt tttatttaat acattaaatt caatggcaag catagcacta gatgatggta
     9301 gttatgattt gtatagtgca ataatagatt tatcaagaat gtttagatat acaatgaggt
     9361 ctgatttaag atttgtagaa ttagaatctg aaatattata cattaaaaat tatttgaatt
     9421 tacagaaatt acgttatgga gatgcactta aagtaaaata tcttattcca gagaagttat
     9481 ataacctttc tgtgcctttt aactttattc aacctattgt agagaatgca tttacacatg
     9541 gatttagaga tattgacaca gaaaagcgaa ttgaaataat tgcaagatta gattctcagt
     9601 atgctattat agagattcat aataatggaa ctatcttaga tggaaataat atagacaaaa
     9661 taaaggcagg tattcgtagt aataatggtc atggtttgtc tttaatatat acaaaattca
     9721 catctgctta tggaaataat tttgatatgg atataaagtc gagtgataat gaaggaactt
     9781 acataatgat taaaattcct attgaaaatt ataaggagta tgtatagaac ttgtgaaaaa
     9841 ttaataagtt ttataaatgt taagaaatat attagatgtt tttatgttgt tttatatatc
     9901 ttactttttt atattattaa gctaaaaggg aggtattctc atgagaagag cactaataat
     9961 agatgatgaa gaagctgttc taaaaataat aacacatttt atagaaatga aagatatgcc
    10021 aataaaaatt gttggtaaag caacatctgg tgatgaagca gtagataaaa tcattggttt
    10081 agagccagac atagttttta tagatattca aatgccaata tacaacgggt tagaagtaat
    10141 agaaaaaaca tctcatttat ctaaaaaaat aaactttgta gtaataactg catttaacta
    10201 ttttgaatat gctcaaaaag ctttgagatt aaatgtaaaa gatatactcc ttaaaccatt
    10261 ggatatgaaa gaatttacta agtctgtaga aaaaattatt ggatatcaat ataccaataa
    10321 tgatttatta aatgagatac tggaatatat aaatctaaac tatagcaatg acttaaagtt
    10381 aaatgattgt gctagattat ttttaaccaa tccatctaat atatctagga tttttaaaag
    10441 taatttaaat acaactttta tatcatatct aaatcatata agaatagaaa aatctattga
    10501 gttacttgaa aatactacaa aaagtataaa tgagattgca gaaatagttg gatataatag
    10561 cctaaataac ttttataaaa actttaagat agaaaaagga atgactccaa aagtttataa
    10621 actgaacact aaaaattgaa attgataaat aaaaagtcaa tatagataat tgtttgagtg
    10681 tgtctgaata agtatacttt aattatgata aaaattaaag cgctttaaac atatttaata
    10741 atattaaacc ttataaaagc tgtacctatt tatataaaaa aatatatata aataaaaaga
    10801 aaaagatata caaaataaaa aaagtgaatt aaatactgtt tcaaaaagac agaattttca
    10861 tttaatttac tgagagttta tatttcttaa aggaaggtaa ctttataatg gaggtaattt
    10921 aatatgtcag acaaagtgaa tatgaaaaat attatctgta ttgcaggcgc ttatctagca
    10981 acagcaattg gttctgggtt tgctactggt caagaaattc tacaattttt tgcctcacaa
    11041 gggtatatgg gtttaattgg agcattaata tcaatggtat tgttttcatt tcttggtgct
    11101 gaagttataa ctagaggtag agaattaaaa cttaaagaac caacaagaat ttacactttt
    11161 tattgtggga agattttagg aaaattttac gaatggttta gtccaatatt tttatttggg
    11221 attcttatag taatgatttc aggtgcaggc tcaactttaa ctgaatacta tggtataaat
    11281 ccttacttag gaagagtatt gatggctgtt gctactttaa ttacagtgtc tatgggttta
    11341 gaaaaattag taagcatact tggacgtatt ggaccagtga tagcagtatt tacaattgct
    11401 gttggattaa taagcttgtt taagcatttt gatggtttat ctatggttgg agaagctatg
    11461 aaaaatattg aagttacaaa agcatcagaa agtccaatcg tttctggtgt aatatataat
    11521 acattaaata ttattgtaat gattgttttt ttaacaggaa tgggagcttc tattgaaagt
    11581 aaaaaggatg ctttttgggg aggtttatta ggtggaatag tgtttatgac tgctggtata
    11641 gttatgtatt tggctatgat ttcagatata ggaaatttgt atatgaaaga aataccatca
    11701 ctttatttag cagataatat atctcctatt gtaggtgtat gtttttcagt tgcattaata
    11761 ttaggtatct acacaacagc tgttccactt ttatggtctg taaccaatag aattgttgaa
    11821 gatgaacatc ctaaatttaa attggtaaca acagtaatag ctattttggc ttgtatagga
    11881 ggttttttac cgtttggtaa actagtaaac attttatatc catatacagg ttatatgggg
    11941 cttttaattt tagtctgtat ggtgtataaa gcaattacat gtagaaaaaa atcaacacaa
    12001 agtcaggaat agagccactt attaatagtt tttatttaaa gaaatttttt atataatccg
    12061 aatttaaaaa gtcaaaatag ttaaataa
//
LOCUS       DAYAPA010000127        11753 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.130, whole genome shotgun sequence.
ACCESSION   DAYAPA010000127 DAYAPA010000000
VERSION     DAYAPA010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11753
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.130"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(87..989)
                     /locus_tag="ACGI59_003162"
     CDS             complement(87..989)
                     /locus_tag="ACGI59_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFG9740938.1"
                     /translation="MDIKQLKYFVEIVKSGFNLSIASKTLHISQPALSQIIKNFEESE
                     DVYLFERYKGRLNGLTPAGERFYVNAEHIINEYKNMMEDLREDSVQFKGKVRIGIPPL
                     ILGIAFSDVVAQLVANNPDIEFDIVEKGAYDLNRMLILQELDYAVLLHPHKIDKSVVT
                     EHVLQEDELTAFLNINHPLAKKDKIDWNDLNEQLLAIFNPTFMIHHKLMQKFTDENVK
                     LKRYIMSGSWDFLLLSTTNSDFITILPSPVHDFFENSKIIEKPFNHPITWKVILCRPK
                     KERYSHVDQHVFKFIIDFFSKKNS"
     gene            1344..2522
                     /locus_tag="ACGI59_003163"
     CDS             1344..2522
                     /locus_tag="ACGI59_003163"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HFG9740939.1"
                     /translation="MKDVVIVSAVRTPIGSFGGVFKNTSAVQLGTIAVKEAISRVRLN
                     LSEIDEVIIGNVLQTGLGQNVARQIAINAGIPNSVPSYTVNKLCGSGLKSVQLAAQSI
                     TSGENDVVIAGGTENMSQAPYIVPTARFGSKMGDITMVDSMLTDGLIDAFNQYHMGIT
                     AENIATKFEFTREMQDKLALESQNKAENAIKNNRFKEEIVPVDVLIRRGKIETIDKDE
                     YPKLGMTFEGLSKLKPAFKKDGTVTAGNASGINDGAAMLILMSQQKADELGIRPLAKI
                     KSYASAGVEPEVMGTGPIPATRKALKKAGLSINDIDLIEANEAFAAQALAVKNELQID
                     SSKLNVNGGAIALGHPIGASGARILVTLIYEMQKRKVETGLATLCIGGGQGISMVVSR
                     "
     gene            2531..3187
                     /locus_tag="ACGI59_003164"
     CDS             2531..3187
                     /locus_tag="ACGI59_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HFG9740940.1"
                     /translation="MNKIVSIDEALSHVKDGDTIMVGGFMANGSPENLIDYLCSKNIK
                     DLTLICNDTGFIDKGVGKMVVNKQFKKIIASHVGLNKETGRQMNNNETDVELVPQGTL
                     AEQIRAAGYGLGGILTPTGIGTLVEEGKQKIVVDSKEYLLEKPIYADVALLFASKVDK
                     AGNLVYKGSMNNFNNLMASAAKITIVEADEIVEIGDIDPNEVNTPGIFVNYIVEGGNL
                     "
     gene            3187..3852
                     /locus_tag="ACGI59_003165"
     CDS             3187..3852
                     /locus_tag="ACGI59_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HFG9740941.1"
                     /translation="MDKLEMQEYIANRVSKELKDGAVVNLGIGLPTKVANYIPDNVNV
                     ILQSENGFLGLGSAEDGNSSDETIVNAGGQPVTILPGGCFFDSATSFGIIRGGHVDIT
                     VLGALQVDKYGNIANYMIPGKMVPGMGGAMDLVTGAKKVIVAMEHTSKGSAKILNNCT
                     LPLTATNAVDLIVTEMGVMEVTSDGILLKEINPAFTLDDVISATEAPLILSDSLNGKI
                     SVV"
     gene            3957..4733
                     /locus_tag="ACGI59_003166"
     CDS             3957..4733
                     /locus_tag="ACGI59_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HFG9740942.1"
                     /translation="MVKDKVVFVTGAASGIGKQIAESFLKNGAKVMFSDINQEALNNT
                     ITELQKKGYDCMSVKCDVTKEEEINHAIDKTVEKYGRLDILINNAGLQHVSMIEDFPT
                     DKFEFMIKIMLTAPFIATKKAFPIMKKQGFGRIINMASINGVIGFAGKAAYNSAKHGL
                     IGLTKVAALEAADSGITVNALCPGYVDTPLVRGQFNDLSKTRNIPLENVLEEVLYPLV
                     PQKRLLDVQEIADYAMFLASDNAKGITGQACLLDGGYTAQ"
     gene            4795..6129
                     /locus_tag="ACGI59_003167"
     CDS             4795..6129
                     /locus_tag="ACGI59_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HFG9740943.1"
                     /translation="MSIIAPIGIIIGIIAIIYFSVKEIHITIAAPIATLIVVILNKMD
                     VVTSMLGADKNNYMGALGNYIMSYFAIFLLGSILAKFMEESGATVSIADFILNKFGSN
                     HPYRVLVAIFIVSFILTYGGISLFVVMFAVIPLARTLFKKLDISWNLIQIPLWLGIAT
                     ITMTIIPGTPAIQNVIPIQYLNTSLTAATIPSIVGSIGCATFGLVYMKYALNKSIRKG
                     ETYATYTSELEEESLDRELPGFFASIFPLLALVIIAITGSTFGSEFMKKNVIYIALIV
                     AILLSAFLFHKFLSSKIQTLSIGASGSIGPIFATSSAVAFGAVIMAAPGFDVFSKLIM
                     SIPGSPLISLTVLTSTMSAITGSSSGALGIVMPNFADYYLSTGLNPELIHRVATIASN
                     ILTIVPQSGVFLTFLSLSKLTHKTGFKETFIVVAVGSLIAEIIVITLGLLMY"
     gene            6625..7557
                     /locus_tag="ACGI59_003168"
     CDS             6625..7557
                     /locus_tag="ACGI59_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HFG9740944.1"
                     /translation="MMRRNIPTKLKTILALGVLTLATASSAVTASASSLENNVNISNK
                     SLVYSNTKYKFSAKPNCSVKPELKPDKDNSCKDKNHNDKNCNNTNKPEDNNNSDSTNK
                     PDNNKPESNKPEDNNNSGSTNKPNNNKPESNKPEDNNNSGSTDKPDNNKPESNKPEDN
                     NNNNSDSTNKPDDNNNSGSTSESFSAYQKEVVDLVNIERAKAGLNPLTLDSSISNVAT
                     KKSQDMIDNNYFSHNSPTYGSPFDMLKKFGISYKTAGENIAMGQKTPKEVVNAWMNSE
                     GHRKNIMNPNFSKIGVGVAQKSGGSIYWTQIFVG"
     gene            complement(7949..11488)
                     /gene="nifJ"
                     /locus_tag="ACGI59_003169"
     CDS             complement(7949..11488)
                     /gene="nifJ"
                     /locus_tag="ACGI59_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFG9740945.1"
                     /translation="MAKFMKTLDGNTAAAHVAYAFTDVAAIYPITPSSTMAEVVDEWA
                     SQGRKNIFGQTVNVVEMQSEAGASGTFHGSLQGGALTSTFTASQGLLLMIPNMYKVAG
                     ELLPGVFHVSARALASQALSIFGDHQDVMAARQTGCVLLASGSVQEVADIAPVAHLAA
                     IEGRLPFIHFFDGFRTSHEIQKVELLENEDYASLLNFEAVQAFRDNALSPNHPVARGT
                     AQNPDIYFQTREASNKYYQNIVGIVEKYMEKMSNLTGRKHSLFDYYGAEDAKYVLIAM
                     GSVTETIEETIDYLNAKGEKYGLVKVHLYRPFSMKHFLDAMPSTVERICVLDRTKEPG
                     STGEPLYLDVRDVFYGKENAPMIIGGRYGLGSKDTTPSDIKTVFDNLVSEQPKNGFTV
                     GIVDDVTNTSLTPSEPIKIASKGTIRCKFWGLGSDGTVGANKQAIKIIGDHTEKYAQA
                     YFDYDSKKSGGITMSHLRFGDTPIRATYLIDEADYIACHKQSYVYQYDLLKGLKKGGT
                     FVLNTIWDQAGLEEHLPAHMKQYIAKNDIKFYTVNAVKLGQEIGLGNRINMIMQSAFF
                     KLAEIIPEEDAVKYLKDSIVKAYGKKGEKIVNMNYAAVDAGINALVKVEVPASWENAV
                     DADKGEVKEPEFIKNILRPMTAQEGNNLPVSTFNGIEDGTFPCGTAAYEKRGIAVDVP
                     EWIIDNCIQCNQCSFICPHSCIRPVLVTEEELANAPEGFEAKKALGKGLEGLKYRMQL
                     SPLDCTGCGNCADICPAKEKALVMKPIDTQLDTELDNWAFAVNPDEVAPKGDVMPVNT
                     VKGSQFRQPLMEFSGACAGCGETAYIKVVTQLYGDRMMIANATGCSSIWGASAPSIPY
                     TCNHEGKGPSWANSLFEDNAEYGFGMYTAVKQIRNKIVDAMTELVSMGICEDAKAVFT
                     EWLDSRNDGEASKVASAKVVELLEKPACDCTDEKAKELVKAIKDRKDYLVKRSQWILG
                     GDGWAYDIGYGGLDHVLASGEDVNVLVFDTEVYSNTGGQASKATPAAAMAKFAASGKK
                     SRKKDLGMMAMTYGNVYVAQVAMGADKNQFIKAVIEAEKHDGPSLIIAYAPCINHGLK
                     EGMGRTVANEAQAVACGYWHLYRFNPEVKAEGKNPFTLDSKEPTASFKDFILAQVRYS
                     AIAKQFPEEADGLFAKAEADAKERYEGYKKLAE"
BASE COUNT         4064 a         1881 c         1786 g         4022 t
ORIGIN      
        1 attattgctc tcaaacatat agatgaaaaa ctaatagttt attataataa aaattgattt
       61 atttatttgt tattttaata acatttttaa gagttctttt tactaaagaa atctataata
      121 aatttaaata catgttggtc tacatggcta taacgttctt ttttaggtct acataatata
      181 actttccatg taataggatg attaaaaggc ttttctataa ttttgctatt ttcaaaaaaa
      241 tcatgaactg gtgatggtaa aattgttata aaatcagaat ttgtggttga tagcaacaaa
      301 aaatcccatg aaccagacat tatatatctt tttaacttaa cattttcatc agtaaatttt
      361 tgcattagct tatgatgtat cataaaagta ggattaaata ttgcaagtaa ctgctcattc
      421 aaatcgttcc aatctatctt atccttctta gcaagtggat gatttatatt taaaaatgct
      481 gtaagttcat cttcttgaag cacatgttca gttactacac ttttatcaat tttatgtgga
      541 tgtaacaata ctgcataatc taattcctgt aagataagca ttctatttaa gtcatatgca
      601 cccttctcta ctatatcaaa ttctatatct ggattatttg caactaactg tgcaactaca
      661 tctgaaaatg ctattccaag tataagtgga ggtattccta ttctaacttt tcctttaaat
      721 tgaactgaat cttctcttaa atcttccatc atatttttgt attcatttat gatatgttct
      781 gcatttacat aaaatctttc tcctgctggt gttaacccat ttaacctgcc tttatacctt
      841 tcaaataaat atacatcttc actttcttca aaatttttta taatttgact taatgctggt
      901 tgagatatgt gaagtgtctt agatgctatt gataaattaa aaccagattt cactatttca
      961 acaaaatatt tcaactgttt tatatccatt ttaaaccccc ttccccaaaa gtataattta
     1021 gattatattt tttcttctaa tgatatttta taggaaaatc aacataattt cattaatatt
     1081 ttaacaaaat atttttctaa atctactata aatttttatc ctataaattt ttcttataca
     1141 ctatataaaa aattagactt agacaaaacg cttttttcgc cgtaatctat aattagaaac
     1201 aggaaaacct aaagcattag gaataaaagt aattgaaata cttaaaaaca agcttgtatt
     1261 aaaaaagtac tttattatag tgttccctaa tttctttatt cagaaaattt ttaattatca
     1321 ttcaagaaag gtaactatta gatatgaaag atgtagtaat cgtatcagca gtcagaacac
     1381 ctataggaag ttttggaggt gtatttaaaa atacttcagc tgttcaactt ggaactatcg
     1441 ctgttaaaga agcgatatca cgtgtaagac ttaatttatc tgaaatagat gaagtaataa
     1501 ttggaaatgt tcttcaaaca gggcttggac agaatgttgc tagacaaatt gctataaatg
     1561 caggtatacc aaattctgtt ccaagctaca cagtaaataa attatgtggt tcagggctta
     1621 aaagtgtcca gctagctgcc caatcaatca catctggtga aaatgatgtg gtaattgcag
     1681 gtggtactga aaatatgagc caagctcctt atatcgttcc aactgctcgc tttggtagta
     1741 aaatgggtga cataaccatg gtagatagta tgctaacaga tggccttatt gatgctttca
     1801 atcagtatca tatgggaata actgcagaaa atattgcaac taaatttgaa tttactagag
     1861 aaatgcaaga taaattggca ttagaaagtc aaaataaggc tgagaatgct ataaaaaaca
     1921 accgctttaa agaagaaatc gttccagtag atgttttaat acgtcgagga aaaatagaaa
     1981 ctattgataa agatgaatat cctaaattag gtatgacatt tgaaggttta tctaaactta
     2041 aacctgcatt caaaaaagat ggtactgtaa cagctggaaa tgcctcaggt attaatgatg
     2101 gtgcggctat gttaatacta atgagccaac aaaaagcaga cgaactaggt attagaccac
     2161 ttgctaaaat aaaatcctat gcttcagctg gtgttgaacc tgaagtcatg ggtactggtc
     2221 caatccctgc aactagaaaa gcactgaaaa aagctggact tagcatcaat gatatagact
     2281 taatagaagc aaatgaagcg tttgctgccc aagcattagc tgttaaaaat gaacttcaaa
     2341 tagattcatc aaagttaaat gttaatggtg gtgcaatagc attaggacat ccaataggag
     2401 caagtggagc tagaatactt gttaccttaa tttatgaaat gcaaaaacgt aaagttgaaa
     2461 ctggacttgc aacactttgt attggtggtg gtcaaggaat atcaatggtg gtttcacgat
     2521 aggaggaaat atgaataaaa tagttagcat tgatgaagct ctatctcatg taaaagatgg
     2581 agatacaata atggttggtg gattcatggc caatggttca ccagaaaatt taattgatta
     2641 tttatgtagt aaaaatataa aagatttgac actaatttgc aatgatactg gctttattga
     2701 caaaggagtt ggcaaaatgg ttgtaaataa gcaatttaaa aagataattg cttctcatgt
     2761 aggattaaat aaagaaactg gaagacaaat gaataacaat gaaacagatg ttgagttagt
     2821 tcctcaggga actcttgctg aacaaatacg tgcagctggt tatggtctag gaggtatact
     2881 cacaccgact ggtattggta ctcttgttga ggagggaaag caaaaaattg ttgtcgatag
     2941 caaagaatac cttcttgaaa aaccaattta tgcagatgtt gctcttctat ttgcatcaaa
     3001 ggtcgataaa gcaggtaatc tagtttataa aggctctatg aacaatttta ataatttaat
     3061 ggcatctgct gccaaaatta caatagttga agcagatgaa atcgttgaaa ttggtgatat
     3121 tgacccaaat gaagttaata caccaggtat ttttgtgaat tacatcgttg aaggaggtaa
     3181 tttataatgg ataaattaga aatgcaagaa tatattgcaa atagagtatc taaagaatta
     3241 aaagacggag ccgttgtaaa ccttggaata ggtctaccaa ccaaagtagc aaattatatt
     3301 ccggataatg taaacgttat cttacaatct gaaaatggct ttttaggttt aggttcagcc
     3361 gaagatggaa attcaagtga tgaaactatt gtaaatgcag gtggtcaacc agttactata
     3421 cttcctggag gatgtttctt tgatagtgct acttcttttg gaataatcag aggaggtcat
     3481 gttgatataa ctgttcttgg cgcacttcag gttgacaaat atggcaatat agcaaactat
     3541 atgattccag gtaagatggt tccaggtatg ggtggagcta tggacctagt aactggagct
     3601 aaaaaagtta ttgtagcaat ggaacatact tcaaaaggtt ctgcaaaaat attaaataat
     3661 tgtactcttc cacttactgc aacaaatgct gtagatttga tagttactga aatgggagtt
     3721 atggaagtaa catcagatgg aattttactt aaagaaataa atccagcttt cacattagat
     3781 gatgttattt ctgctactga agctccttta atattgtcag attcattaaa tggaaaaata
     3841 tctgttgttt aaaataatta aaactagaaa ataaaaatct cttagacgta ttacatataa
     3901 atttgtattg cttaaaatat taatataaaa ataaaataaa attggaggaa atgaaaatgg
     3961 ttaaagataa agttgtattc gtaacaggag ctgcaagtgg aataggtaaa caaatagctg
     4021 aatcgttctt aaaaaatggt gctaaagtaa tgttctctga tataaatcaa gaagctttaa
     4081 ataataccat aacagagtta caaaaaaaag gttatgattg catgagtgta aagtgtgatg
     4141 taactaagga agaagaaatt aaccacgcta tagacaaaac tgttgaaaag tatggcagat
     4201 tagatatatt aataaacaat gctggacttc aacatgtttc tatgatagaa gatttcccaa
     4261 ctgataaatt tgaatttatg ataaaaataa tgctgacagc accttttata gctactaaaa
     4321 aagcatttcc tataatgaaa aaacaaggct ttggtcgtat aataaatatg gcctcaatca
     4381 atggtgtaat aggatttgct ggaaaggctg cttataactc agcaaaacat ggacttattg
     4441 gactaacaaa agttgctgct cttgaagctg ccgattctgg tataacagtt aatgctttat
     4501 gccctggata tgtagatact ccacttgtca gaggtcagtt caatgacctt tcaaaaactc
     4561 gtaatatacc attggaaaat gtcttagagg aagttttata cccacttgta ccacaaaaac
     4621 gcctattaga tgttcaagaa attgccgact atgctatgtt cttagcaagt gataatgcta
     4681 agggtattac aggacaagct tgcttactag atggaggata tactgcacaa tagttattat
     4741 atttagggct tgaagaaagt attcttcagg tccttctact aaggagggat atttatgagt
     4801 attatcgcgc caattggtat aattattggt atcatagcta ttatatattt ttcagttaaa
     4861 gaaattcata taactatagc tgctccaata gcaactttaa tagttgttat tttaaataaa
     4921 atggatgttg taacgtcaat gctaggtgct gacaaaaaca attatatggg tgcacttgga
     4981 aactatataa tgagttattt tgcaatattc ttattaggct caatacttgc taaatttatg
     5041 gaagaaagtg gagctactgt atcaatagct gattttattt taaataaatt tggctctaat
     5101 catccttata gagttttagt tgctatattt atagtaagtt ttatattaac gtatggtggt
     5161 ataagcttat ttgtagttat gtttgcggtc attccacttg caagaactct atttaaaaaa
     5221 cttgatattt cttggaacct tattcagata ccactttggc ttggtattgc aacaattact
     5281 atgactataa ttcctggaac accagccatt caaaatgtta tacctattca atatctaaat
     5341 acgtctctta ctgcagctac tataccaagt attgtaggta gtataggttg tgctacattt
     5401 ggacttgtct atatgaaata tgctctaaac aaaagtatta gaaaaggtga aacttatgca
     5461 acttacactt cagaattaga agaagaatct ttagatagag aattaccagg attttttgca
     5521 agtatttttc ctctattagc actcgtaatt atagctataa ctggaagtac ttttggttca
     5581 gaatttatga aaaaaaatgt aatttacata gctttaatag ttgcaattct tttatctgct
     5641 tttttattcc ataaatttct atcatctaag atacagacat tgagcatagg tgctagtggt
     5701 tctataggtc ctatatttgc tacatcttct gcagttgcat ttggagcagt aataatggct
     5761 gcacctggtt ttgatgtatt tagcaaactt ataatgtcaa taccaggtag tccacttata
     5821 agtcttactg ttttgacttc aacaatgtca gcaataacag ggtcctcttc tggtgcatta
     5881 ggaatagtaa tgccaaactt tgcagattat tatttaagta ctggtctaaa tccagaatta
     5941 attcaccgtg ttgctacaat agcttcaaat atactaacta tagttccaca aagtggtgtt
     6001 tttcttacat tcttaagctt atctaagtta acacataaaa ctggatttaa agaaactttc
     6061 attgtagttg cagttggttc actaatagca gaaattattg taattacatt aggattacta
     6121 atgtattaat ataaaacaag ttaaaattca taatatgaaa ggtggtgtct caaagtgaac
     6181 tttttactta atgagacact ctttttatat gaaataacat atgcctatca tttaaacaat
     6241 aaaaaaggcc ttatctctat tttgagacag acccttttta tttcttaaag atttaaataa
     6301 ttataatttg ttgctaatac gaaagcatct actgaataaa tttaaccaaa taaaaaaaaa
     6361 tttatcataa ctataaattt acgttatgga attattataa aaatattatt ttctctaact
     6421 aagtaattcc atactataca ggcttttaat acaaattgtc aaacacactg aaatatacct
     6481 atttattcca tatatatctt ttttacatat ctttacagta aaaaaagtct taatcttatg
     6541 ttagtataga ttcacgggta accccctaca ctaaaggtta caaatcagta acatttttag
     6601 aatttttaga ttaagaggtg tttaatgatg agaagaaata taccaacaaa attaaaaact
     6661 atattagctt taggagtgtt aactttagca acagcttcaa gtgctgttac tgcaagtgct
     6721 tcatcacttg aaaataatgt aaatataagt aacaaaagtt tagtatatag taatactaaa
     6781 tacaagtttt ctgcaaaacc aaattgttct gtaaaaccag aacttaagcc tgataaagat
     6841 aactcttgta aggataagaa tcataatgac aagaattgca acaacactaa taaacctgag
     6901 gacaacaata attcagactc tactaataag cctgataaca ataaacctga gtctaataaa
     6961 cctgaagaca acaataattc aggctctact aataaaccta ataataataa acctgagtct
     7021 aataaacctg aagacaacaa taattcaggc tctactgata aacctgataa caataaacct
     7081 gagtctaata aacctgaaga caacaataac aataattcag actctactaa caaacctgat
     7141 gataataaca actcaggctc tacaagtgaa agtttttcag cttaccaaaa agaagttgtt
     7201 gacttagtaa atatagaaag agctaaagct ggattaaatc ctcttactct tgattcttct
     7261 atatcaaatg ttgctacaaa aaaatctcaa gatatgattg ataacaacta tttttcacac
     7321 aattctccaa cttatggttc tccatttgac atgttgaaaa aatttggaat aagctataag
     7381 acagcaggtg aaaatatagc tatgggtcaa aaaactccta aagaagttgt aaacgcatgg
     7441 atgaattctg aaggtcacag aaaaaatata atgaatccaa atttcagtaa aataggtgtt
     7501 ggagtagcac aaaaaagtgg tggttcaatt tattggacac aaatctttgt aggataaaat
     7561 actctttttc catacatatc aaaagagtat ctctaagtga taaaaaacac aaatgagata
     7621 ctcttttgta tctattttaa aattatcaaa tccaaatata catactataa atattatttg
     7681 ttctcttaat tttagtgtgt agaacaatat ataaaaagta ttggcaagat gaaaatggaa
     7741 aacttaaaaa agatatttaa taggtaaaaa caaaatctta atatcttcat aaaaaaaggc
     7801 tgtctcaaaa tagagataag gcctttttca ttgttgaaat gattaaaata tatttgattt
     7861 catacaaaaa gagtgcctca tgaactaaaa agctcacttt gagacactct cttttatcaa
     7921 tttatataag cattataata ctttaatatt attcagctaa ttttttgtat ccttcatatc
     7981 tttctttagc atcagcttca gctttagcaa ataatccatc agcttcttct gggaattgtt
     8041 tagctatagc agaatatctt acttgtgcaa gtatgaaatc tttgaagctt gcagttggtt
     8101 cttttgaatc taaagtgaat gggtttttac cttcagcttt tacttctgga ttgaatctgt
     8161 ataaatgcca gtatccgcaa gcaacagctt gtgcttcgtt agctacagtt cttcccatac
     8221 cttcttttaa tccatggttt atacatggag cataagctat tattagagat ggcccatcat
     8281 gtttttcagc ttctataacg gctttgataa attggttttt atcagcaccc atagcaactt
     8341 gagctacata tacgtttcca tatgtcatag ccatcattcc taaatctttc tttctagatt
     8401 ttttacctga agcagcaaac ttagccatag cagcagctgg agtagcttta gaagcttgac
     8461 ctcctgtatt tgaatatact tctgtatcaa atacaagtac gtttacatct tcaccagatg
     8521 caagaacatg gtctaatcca ccgtaaccga tatcatatgc ccaaccgtct ccacctagta
     8581 tccattgaga tctcttaact agataatctt ttctatcttt tatagctttt actaactctt
     8641 tagctttttc atctgtacaa tcacaagctg gtttttctaa taattcaaca accttagcac
     8701 ttgctacttt agaagcttct ccatcatttc tagaatctaa ccactctgta aatactgctt
     8761 tagcatcttc acagataccc atagaaacta actctgtcat agcatctact attttatttc
     8821 ttatttgctt aacagctgtg tacataccaa atccatattc agcattatcc tcgaataaag
     8881 agtttgccca agatggacct ttaccttcat ggttacaagt ataaggaatt gatggtgcag
     8941 aagctcccca tatagaagaa catccagtag cattagctat catcattcta tctccatata
     9001 attgtgtaac tactttaatg tatgcagttt ctccacatcc agcacatgct cctgaaaact
     9061 ccattaatgg ttgtctaaat tgacttcctt taacagtgtt tacaggcata acatcacctt
     9121 ttggagctac ctcatcagga tttactgcaa atgcccaatt atctaactct gtatcaagtt
     9181 gagtatctat tggcttcata actaaagctt tttctttagc tggacatatg tcagcacagt
     9241 ttccacatcc tgtacagtca agtggactaa gttgcattct atattttaat ccttctaatc
     9301 ctttacctaa tgctttttta gcttcaaatc cttctggagc attagctaat tcttcttcag
     9361 taactaatac tggtcttata catgaatgag gacatataaa agaacattgg ttacattgaa
     9421 tacagttatc tattatccat tcaggaacat caacagctat accacgtttt tcataagctg
     9481 cagtaccaca agggaatgtt ccatcttcta taccattgaa tgtacttact ggtaagttgt
     9541 ttccttcttg tgcagtcatt ggtcttagta tattttttat aaattctggt tctttaactt
     9601 ctcctttatc tgcatcaaca gcattttccc aagaagctgg aacttcaact ttaactaaag
     9661 catttattcc tgcatcaaca gcagcatagt tcatgttaac tattttttca ccttttttac
     9721 cataagcttt aactatagaa tcttttaagt atttaacagc atcttcttct ggtattattt
     9781 cagctaattt aaagaatgca gattgcatta tcatatttat tctgtttcca agacctattt
     9841 cttgtcctag tttaacagcg ttaactgtat agaattttat atcattttta gctatatatt
     9901 gcttcatatg agctggtaaa tgttcttcaa gtcctgcttg gtcccatata gtgttaagta
     9961 caaatgtacc acctttttta agacctttta ataaatcata ttgataaaca tatgattgtt
    10021 tatgacaggc aatataatca gcttcatcta ttaaataagt agctcttatt ggagtatcac
    10081 caaatcttaa gtgagacata gttataccac cagatttttt agagtcataa tcaaagtaag
    10141 cttgagcata tttttcagta tggtcaccaa ttattttgat agcttgttta ttagctccaa
    10201 cagtaccgtc agaacctaat ccccagaact tacatcttat agttccttta gaagctattt
    10261 ttattggctc agatggagtt aaagaagtat tagttacatc atcaactata cctactgtaa
    10321 atccattttt aggttgttct gaaactaagt tatcaaaaac agtttttata tctgaaggag
    10381 tagtatcttt tgaacctaat ccgtatcttc cacctattat cataggagca ttttcttttc
    10441 catagaatac atcacgtaca tctaagtata atggttcacc agttgaacct ggctctttag
    10501 ttctatcaag tacacatatt ctttcaacag tagatggcat agcatctaag aaatgtttca
    10561 ttgagaatgg tctatataaa tgaactttaa ctaaaccata tttttctccc ttagcattta
    10621 agtaatctat agtttcttct attgtttctg ttactgaacc catagctatt aatacgtatt
    10681 tagcatcttc tgctccatag taatcaaata aactatgttt tctacctgtt aaattactca
    10741 ttttttccat gtatttttca actataccta ctatattttg atagtattta ttagaagctt
    10801 ctctagtttg gaaatatata tctgggtttt gagcagtacc acgagcaact ggatgatttg
    10861 gagataaagc attatctcta aatgcttgaa ctgcttcaaa gtttaataaa cttgcataat
    10921 cttcattttc tagtaattca actttttgga tttcatgtga agttctgaat ccatcaaaga
    10981 aatgtataaa tggtaatcta ccttctattg cagctaaatg tgcaacaggt gctatatcag
    11041 ctacttcttg aactgaacca gaagctaata atacacatcc agtttgtcta gcagccataa
    11101 cgtcttggtg atccccaaat attgataacg cttgagatgc taaagcacga gcacttacat
    11161 ggaatactcc tggtaataac tctcctgcaa ctttatacat gttaggtatc attaataata
    11221 aaccttgaga tgctgtaaaa gtagaagtta aagctcctcc ttgtaaagaa ccgtggaatg
    11281 ttcctgatgc acctgcttct gactgcattt caacaacatt aactgtttgt ccaaatatat
    11341 tttttcttcc ttgtgatgcc cactcatcaa ctacttcagc catagttgaa gatggtgtga
    11401 ttggatatat agctgctaca tctgtaaacg cataggcaac atgagctgca gctgtatttc
    11461 catcaagtgt tttcataaac ttagccataa tatgtatcct ccttgatagc tacaattgta
    11521 cttgtttata acaagtctat ttttttatat ttacatataa tttacacaac aaaactataa
    11581 acacacatat agtcgcgcaa atactctatc ttctaatata atactacaga ttagataaaa
    11641 ttcctttgaa aattgtaaat taattacata tttaagtaga ttttttttaa aattaaatat
    11701 gttaagtttt tttcataatt ttgtatacaa tatataataa attaagttag att
//
LOCUS       DAYAPA010000128        11528 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.131, whole genome shotgun sequence.
ACCESSION   DAYAPA010000128 DAYAPA010000000
VERSION     DAYAPA010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11528
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.131"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(89..655)
                     /locus_tag="ACGI59_003170"
     CDS             complement(89..655)
                     /locus_tag="ACGI59_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3073 family putative ECF transporter S
                     component"
                     /protein_id="HFG9740946.1"
                     /translation="MDTKKVTFSSMCIVINIVLGMVISMINIPLLFLDTVGTVLGAVV
                     LGPFWGALIGGCTNLVLGVISGPTNIPFALVNIVLGLIVGYISKKKGFGYKEAIITGV
                     ILSIVCPLIGTPISVLLFGGLSGSGADLLVGFLVQSGQKIFTAAFFPRILSNIVDKPL
                     SCIMVVYFISKMPKGFIKQYSKNTSSSY"
     gene            821..1342
                     /locus_tag="ACGI59_003171"
     CDS             821..1342
                     /locus_tag="ACGI59_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3072 family TudS-related putative desulfidase"
                     /protein_id="HFG9740947.1"
                     /translation="MKRNREIVVVCHCILNCNSKVEGLSEFKGAQNIAINLINKGYGI
                     IQLPCPEMIMYGINRWGHVKEQFDNLFFREQCRQMLIPHVRQFDEYLKNDYDIKSIIA
                     IDGSPSCGYNKTCSSKRWFGELSGCNELMDKIKDIQMVDGKGIFIEELEKLLKEYNLE
                     IPIVGFDELREDK"
     gene            2141..4219
                     /locus_tag="ACGI59_003172"
     CDS             2141..4219
                     /locus_tag="ACGI59_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HFG9740948.1"
                     /translation="MIKISEDYIEKRFDNELLRIEAWGKNSLRIRSFVDQNFVDENYA
                     LNEKPKLNKDDITINKNEDGSAIIKNGKIKAVLDHRDRITFYNDKNEILLKEYIRLRA
                     VKHDDGGEDVGTIEITKDFNSTLKLKSREYRPSYSGEFEVTTRFESEPSEKIFGMGQY
                     QHKFLDLKNTVLELAQRNSQISVPFYISSLGYGLLWNNPGIGKVSFAKNMTEWKMFST
                     NFIDYWITCGESPKELNKNYSQVTGTVPMMPENLLGLWQSKLRYRTSEEVLDVVKEYS
                     KRGIKLSSIAIDYFHWPKQGEYKFDLDYWKNPKELVKKLKEEYSVEPIVSVWPTVQSD
                     AENYNDYLENGYLVNVNRGVRMTMQIQGNTVFVDMTNENAREYVWDRIDKNYKQLGID
                     YYWLDVAEPGYSVYDFDNYRYKKGNVLSCGNIYPIDYLKMIYDGLHNDIESVVTLVRG
                     AWAGAQKYGALVWSGDIDSSFEAFNNQVNTGLNMGLAGIPWWTTDIGGFHGGNPKDPE
                     FRELMVRWFQYATFSPILRMHGDRLPHSKPLSNKGGGSMVTGAPNEIWSYGEEVEVIL
                     TKFIKIRESLKTYLKKLMKEAHEDGTPVMRTLFYEFPEDDKTWEVDNTYMLGDEILVA
                     PIMNYKDRSRKVYLPKGHTWENIFSGVSYEGGKTYEVECPLEEIPIFLKQDSSYNFKE
                     LKNILGEVKS"
     gene            4219..4998
                     /locus_tag="ACGI59_003173"
     CDS             4219..4998
                     /locus_tag="ACGI59_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFG9740949.1"
                     /translation="MEPQLWQLLLIVLYGFFINYEKNSTMFGTYQPVTAGFITGLILG
                     DINTGLYIGGTLQLLSLGISNFGGASIPDYQTASIVATFITITTKQEASVGISIGIPV
                     ALLMVQLDVLRNTIGIWLVHKAEDGAKKGNYKNITYMQMLGVLLTAATTGIPVALSVI
                     FGPSLINTILKYTPEWLTGGLTVAGGLLPAVGIGLLLRYLPAKEYFSYLVIGFVLAVY
                     MKVPLLGVALIGGAIALIIYKKNLENQEQQYTVVGGMDEDE"
     gene            4991..5800
                     /locus_tag="ACGI59_003174"
     CDS             4991..5800
                     /locus_tag="ACGI59_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFG9740950.1"
                     /translation="MSDKVSLNKSNEKVKKSVLWRWFFTSSVSSNYEKMQALAYCYAV
                     LPFLKVVYKDNPEKLQSAVLNHLQFFNTNPWVAPYILGINVAMEELSSEETEEAVASI
                     KTGLMGPVAGLGDSLFVVIPWTIFGAIAANMAIDGSPFGILLWIVVSVALKLLSIPLF
                     NAGYTSGTKLISSIEKSLKILTESTSILGLMVVGALIPSVVKANVALNFQQGDFTMKG
                     QEILDQIMPGLVPAILVVIVYWALKKNIKPIYLILGVMVVSIILYALGILK"
     gene            5820..6302
                     /locus_tag="ACGI59_003175"
     CDS             5820..6302
                     /locus_tag="ACGI59_003175"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFG9740951.1"
                     /translation="MEMKGIKNIRIDDRLIHGQVATMWSNKLGVTRLMVVNDAVANNS
                     VQKQVLRMATPAGIASSIITEETAIKNITAGKYEGQNVLLIVKSPVDLIPFVEAGFKI
                     HNINVGNMSSRKDTTVLRPNISVTEEEKEAFKRLLDEGIEITTIMTPDDKKTYLVDIL
                     "
     gene            6323..6727
                     /locus_tag="ACGI59_003176"
     CDS             6323..6727
                     /locus_tag="ACGI59_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFG9740952.1"
                     /translation="MNSIILMSHGNMAEEVLNSAKMIIGDSIDYPTVNMKQDDGIEGT
                     IEKLKKALEVYKESSEIIIMADLLGGTPCNAALLVANNDSRIKIISGLNLGMVIESAF
                     LDTKELAAKLEKIGKDNISMVEQNANLLSDDE"
     gene            complement(6883..8043)
                     /locus_tag="ACGI59_003177"
     CDS             complement(6883..8043)
                     /locus_tag="ACGI59_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFG9740953.1"
                     /translation="MVTDKYTIREMNERLVLEQIIKNGPISRASIASTIGLNKATISA
                     ITKKLIDESLVHEIGIGNSTHSGGRKPILLVFNKCAGISLSMDIGYDYIFSSLSYLDG
                     TIINSKKLTDIQVSKDNVIQLIDEIINSYNISKIDTPYKVIGLTLAIHGITCENKVLF
                     TPYYNLNEIDLYSILSKKYDFPIHIENEANLTALAENTFSTVHNSLLSLSIHSGFGSG
                     IIINNKLYSGRNGMSGEIGHTIIMPNGKLCPCGNRGCLEQYCSEKKVFEQLSSLENIP
                     KIDSDIVKQLYYEDNHNAKKVIHEFCSYLTIAINNAITTYAPEIIYLNSQIISDIPEI
                     LQITKDMLVSSFNKGINIEISSLGSEASLYGGSAVNIKSFLNIQNLTLINEI"
     gene            8251..9801
                     /gene="xylB"
                     /locus_tag="ACGI59_003178"
     CDS             8251..9801
                     /gene="xylB"
                     /locus_tag="ACGI59_003178"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFG9740954.1"
                     /translation="MKLEYVLGVDIGTSGTKTVLFDKLGNTIKSCTYEYPLIQEKSGW
                     AEQDANDWWKAVVESIREVVQSSNISSECIKGIGLSGQMHGLVMLDNEGKTLRNSIIW
                     CDQRTVKECEEITDLVGEERLIEITANPALTGFTASKILWVRNNEPDIYKNTNKILLP
                     KDYIRYKLTGEYATEVSDASGMQLLDIRKRDWSDEVLEKLNIDKNLLGKVYESQEITG
                     YVTRDVSSLTGLKEGTIVVGGAGDQAAGAIGNGIVKDGVVSSTIGTSGVVFAYTKEPK
                     IDKEGRVHTFCHAIPNTWHVMGVTQGAGLSLKWFKDNFCQSEVEVSNSLGEDVYEIIN
                     NQVSKVPTGCNGLLYLPYMMGERTPHLDPYARGVFFGLSPIHSKKEIARSIMEGVSYS
                     LKDCMDIIENLNIDVNEVRASGGGGKSKVWRQMQADMFNQDVYTINSSEGPALGVAIL
                     ALVGAGIYENIQKACDAIIKTSTKLEPISENVDTYNIYHRLYKRIYKSLKDDFKLLDE
                     VVNAKINN"
     gene            9897..11234
                     /gene="xylA"
                     /locus_tag="ACGI59_003179"
     CDS             9897..11234
                     /gene="xylA"
                     /locus_tag="ACGI59_003179"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HFG9740955.1"
                     /translation="MSEIFKGIGQIKFEGVKSDNELAFRYYNPEQVVGNKTMKEHLRF
                     AMSYWHTLCGEGNDPFGVGTVERPWNNITDPIEIAKIKVDAGFEFMSKMGIEYFCFHD
                     RDIAPEGRDLEETNKILDEIVEYIKVNMEKTGIKLLWGTANMFGNPRFVHGASTTCNA
                     DVYAYAAAQVKKAMEITKYLGGENFVFWGGREGYETLLNTNTELEMDNFARFLQMAVD
                     YAKEIGFTGQFLIEPKPKEPTKHQYDFDTATVLGFLRKYNLDKYFKMNIEANHATLAG
                     HTFQHELNIARINNVLGSIDANQGDLLLGWDTDQFPTNIYDATLAMYEVLKQGGIAPG
                     GFNFDSKVRRASFEVEDLFLAYIAGMDTFAKGLLIAHKLLEDEVFENFTKERYASFSE
                     GIGKDIVEGKVGFKELESYALQMPVIKNKSGRQEMLEAILNRYIYEVDTISNK"
BASE COUNT         4449 a         1322 c         2062 g         3695 t
ORIGIN      
        1 atctaattaa attattaata aaaatgttat ataaaaatga gtatatagca ttttaaagag
       61 taataaacct tacataataa atgatttatt aatatgaact agaagtattt ttactatatt
      121 gttttataaa acctttaggc attttactta taaaatatac caccataata caagacaaag
      181 gtttgtctac tatattacta agaatccttg gaaagaacgc agcagtaaat atcttctgac
      241 cagactgaac taaaaaacca accaataaat cagcaccact tccacttaaa ccaccaaaca
      301 ataaaactga aataggagta ccaattaaag gacaaacaat agataaaatt actcctgtaa
      361 taatagcttc tttataacca aaaccttttt tcttagaaat atatccaaca ataagaccaa
      421 ggacaatatt tacaagggca aaaggtatat ttgtaggacc tgatataaca cctaaaacta
      481 agtttgtaca tccaccaata agagcacccc aaaaaggacc taatacgaca gccccaagta
      541 cagtaccaac tgtatctaaa aataataatg gtatatttat catagatata accataccta
      601 agacaatatt aataactatg cacattgaag aaaatgtgac ttttttagta tccaattata
      661 atacctccta aaaaaatata tacatttatc atagtataga ttataaaaga ttactaattg
      721 tatatttcta tatagaatat tggaaatata gaacaaataa ataatataat tatcaaaata
      781 tgtagtatca ttaaaattaa gaataactaa ggagattatt atgaaaagaa atagagagat
      841 tgtagtagta tgtcattgta tattaaattg taattctaaa gtggagggat tgtcagaatt
      901 taagggagca caaaatatag cgataaattt aataaataaa gggtatggaa taattcaact
      961 accatgccca gaaatgatta tgtatggaat aaataggtgg ggacatgtaa aagaacagtt
     1021 tgacaatttg ttttttagag aacaatgtag gcaaatgtta atacctcatg taaggcagtt
     1081 tgatgaatat ttaaaaaatg attatgatat aaaaagtatc attgcaatag atggcagtcc
     1141 aagttgtggt tataataaga catgtagttc taaacgttgg tttggtgagt taagtggttg
     1201 caatgaattg atggataaaa taaaggatat acaaatggtg gatgggaaag gtatttttat
     1261 agaagagcta gaaaagctac ttaaagaata taatttagag atacctatag ttggatttga
     1321 cgagcttaga gaagataagt agagatataa atatttattg tctcattatg aaacaccatt
     1381 atatttggca gaaaaatatg actgatggac agtacaggaa ccatttttaa gtggagtaat
     1441 tagtacttat aaagatgtat ccactagctg aaagaaaatg tagaaaggta ataaatgaat
     1501 taagaagtag ataatatata taaaggagtg tctaaaagtg aactttttag tttatgagac
     1561 actctttttt tatattaaat caagtatatt aaatcaaata tattttaatc attttagcaa
     1621 tgaaaaataa atttatctat attttgagac aacatctttt ttagttttat ttatttgttg
     1681 tcgtttttga cttggtatac aattgtatat tttcttaaat aatttcataa attatttata
     1741 attataaaat ccattgtttt gaacaatatc taatatatta ttatctgtat ttataaggtc
     1801 actatacata ttttcaagtc cgatattatc tacataatag gcaaatgtta ttcctgtatg
     1861 tgttttaaaa aaactagatg tatataggta tcatcacaga tattaaaagt ataaataaaa
     1921 gatattaaga acaactttcc agcaatatat tagaaaattt tactaaaatc cggaaagcga
     1981 ttgacaaaca aagtcaaata aagtatatta ttggtaagtt agtttaatgt acaaatgaat
     2041 tatatataaa acctaattta taaataacga tactaaacga cattatttaa cttaagttac
     2101 acaaagatag aatattatac cattgattta ggaggtagcc gtgataaaaa tatctgaaga
     2161 ctatattgaa aaaagatttg acaatgaact attgcgaata gaagcatggg gaaaaaactc
     2221 tttacgtatt aggtcgtttg tagaccaaaa ttttgttgat gaaaactatg ctcttaacga
     2281 aaaacctaag ttaaataaag atgatattac aattaataaa aatgaagatg gaagtgcaat
     2341 cattaaaaat ggaaagataa aagctgtctt agaccataga gatagaatta ctttttataa
     2401 tgataaaaat gaaatattat tgaaagaata tattcgatta agagctgtaa aacatgatga
     2461 tggtggagaa gatgtaggga caattgagat taccaaagac tttaactcta ctctaaagtt
     2521 aaaatcaaga gaatatcgac caagctatag tggagaattt gaagtaacta ctagatttga
     2581 atctgagcct tctgaaaaga tttttggaat gggacaatat caacataaat ttttagattt
     2641 aaagaatact gttttggagt tagcccaaag aaattctcaa atatcagttc ctttttatat
     2701 ttctagtcta ggttatggac ttttatggaa taatccggga attggaaaag tatcttttgc
     2761 taaaaatatg acagaatgga agatgttttc tacaaatttt atagattatt ggattacttg
     2821 tggagaaagc ccaaaagagt taaataaaaa ttactctcaa gttactggaa ctgttcctat
     2881 gatgcctgaa aaccttttag gactatggca aagtaaacta agatatagaa cttcagaaga
     2941 agttttagat gttgtgaagg aatattctaa aagaggaatt aagctttcat caattgcaat
     3001 tgactatttt cattggccta agcaaggtga atataaattt gacttagatt attggaaaaa
     3061 tcctaaagaa ttagttaaaa aattgaaaga agagtattca gtagaaccaa tagtatctgt
     3121 atggcctaca gttcaaagtg atgcagaaaa ctacaatgat tatttagaaa atggctattt
     3181 agtcaatgtt aatcgtggtg ttagaatgac aatgcaaatt caaggaaata cagtctttgt
     3241 agatatgaca aatgaaaatg caagagaata tgtttgggat agaatagata aaaattataa
     3301 acaacttggg attgactatt attggttaga tgtagctgaa cctggctatt ctgtgtatga
     3361 ttttgataac tatagatata aaaaaggaaa tgttttaagt tgtggtaata tttatcctat
     3421 tgattattta aaaatgattt atgatggttt acataatgac atagagtctg ttgtaactct
     3481 tgtaagagga gcttgggcag gagctcaaaa gtatggagct ttagtatggt ctggtgacat
     3541 tgattctagt tttgaagctt ttaataatca agtaaataca gggttaaata tgggactggc
     3601 aggtatacca tggtggacta ctgatattgg aggtttccat ggaggaaatc ctaaagaccc
     3661 agaatttaga gaacttatgg taagatggtt ccaatatgct actttttcac caattttgag
     3721 aatgcatgga gatagattgc ctcatagtaa accattatca aataaaggtg gaggctcaat
     3781 ggtaactgga gctcctaatg aaatatggtc ttatggtgaa gaagttgaag taattcttac
     3841 aaaattcata aaaattagag agagcttaaa aacttattta aaaaaactga tgaaagaagc
     3901 gcatgaagat ggaacaccag ttatgagaac tttattttat gaatttcctg aagatgataa
     3961 aacttgggag gtggataata catatatgct tggagatgaa attttagtag ctccaataat
     4021 gaattataaa gatagaagta gaaaagtata tttaccaaaa ggacatacat gggaaaatat
     4081 atttagtgga gttagttatg aaggtgggaa aacttatgaa gttgaatgcc cactggaaga
     4141 aattcctatt ttcctaaagc aagattccag ttataatttt aaagaactaa aaaatatttt
     4201 gggagaggtg aaatcataat ggagccacaa ttatggcaat tattattaat cgtactatat
     4261 ggatttttta taaactatga aaaaaattca acaatgtttg gaacatatca accagtaaca
     4321 gcaggtttta ttactggtct tatcttagga gatataaata cagggttata tattggaggt
     4381 acattacagc tattatcatt aggtattagt aactttggtg gagcatcaat acctgattat
     4441 caaacagcaa gtatagtagc cacatttata actattacga ctaagcaaga agcatctgta
     4501 ggaatctcta taggtattcc agttgcttta ttgatggtac aattagatgt tcttagaaat
     4561 actattggta tatggctagt tcataaagca gaagatggag ctaaaaaagg aaattataaa
     4621 aatataacct atatgcaaat gttaggagta ctattaacag cagctacaac aggtattcca
     4681 gttgctctat ctgtaatatt tggaccttct ttaattaata ccattttgaa atatacacct
     4741 gaatggttaa caggtggttt aacagtagct ggaggactac ttccagcagt agggatagga
     4801 ttattactta gatatcttcc tgctaaggag tattttagtt acttagtaat tggttttgtt
     4861 ttagcagtat atatgaaagt accattatta ggtgtagctt taattggtgg agcaattgca
     4921 cttataattt acaaaaagaa tttggaaaat caagaacaac aatatactgt agtaggaggt
     4981 atggacgaag atgagtgata aggtatcatt aaataaatca aatgaaaagg tgaaaaaaag
     5041 tgtcttatgg cgctggtttt ttactagttc tgtttcttca aattatgaga agatgcaggc
     5101 cttggcatac tgttatgcag tactaccttt tttaaaagta gtatacaaag ataatccaga
     5161 aaagttacaa agtgctgtat taaatcattt acaatttttt aacactaatc cttgggttgc
     5221 cccttatatt ttgggtatta atgtagcaat ggaggagtta tcaagtgaag agactgaaga
     5281 ggctgttgct tcaattaaaa caggcttgat gggaccagta gcaggtttag gagatagttt
     5341 atttgtcgtt attccgtgga caatttttgg agctatagca gctaatatgg cgatagatgg
     5401 aagtccattt ggaatcttac tgtggattgt agtgagtgta gcattgaaat tattaagtat
     5461 accactattt aatgctggtt atacatcagg tactaagcta atatcttcaa ttgaaaaaag
     5521 tttaaagatt ttaactgaga gtacatctat tttaggttta atggttgtag gtgcattaat
     5581 accttcagtt gtaaaagcta atgttgcttt gaactttcaa caaggtgatt ttacaatgaa
     5641 aggtcaagaa atattagatc aaattatgcc tgggttagtt cctgcaattt tagttgtgat
     5701 tgtttattgg gctcttaaaa agaatataaa acctatatac ttaattttag gagtaatggt
     5761 tgtttcaata attttatatg cattaggtat tttaaaataa ggaaaggatg atattagata
     5821 tggaaatgaa aggtattaaa aatattcgta tagatgaccg tttaatccat ggtcaagttg
     5881 caactatgtg gagtaataaa ttgggtgtta caagattaat ggtagtaaat gatgcagtag
     5941 ctaacaattc tgttcaaaaa caagttttaa gaatggctac acctgcaggt atagcgtcat
     6001 ctataattac agaagagact gctataaaaa atattactgc aggaaagtat gaaggacaaa
     6061 atgtattatt aattgttaag tctcctgtgg accttattcc atttgtagag gcaggattta
     6121 aaatacataa tataaatgta ggaaatatgt caagtagaaa agatacaaca gttctaagac
     6181 ctaatatcag tgttactgaa gaagagaaag aagcatttaa aagactttta gatgaaggaa
     6241 ttgaaattac aactataatg acgcctgatg ataagaaaac ttatcttgta gacatcttat
     6301 agatttaaag aggtgtatta aattgaatag tattattttg atgagtcatg gcaatatggc
     6361 tgaagaagtc ttaaattctg caaaaatgat aataggggat tctatagatt atcctactgt
     6421 aaatatgaaa caagatgatg gaatagaagg aacaatagaa aaattaaaaa aagctttaga
     6481 agtgtacaag gaaagttcag aaataattat aatggcagat ttactaggtg gaactccatg
     6541 caatgctgct ttattagtag ccaataatga cagtcgaatt aaaattattt cagggctaaa
     6601 tttaggtatg gttattgaat cagctttttt agatacaaag gaattggcag caaaattaga
     6661 gaaaataggt aaagataata tttctatggt tgaacaaaat gcaaatttat tatctgatga
     6721 tgagtaggaa gattatatta atatttaaaa cgaaatactt atgttaatac ttaattgata
     6781 tacaattttt aaaaaatata gctaatcttg cataaactat ataataaatc taattttaga
     6841 atgtataatt taaagggtta tggctgtata tagtcataac ccttatattt cattaattaa
     6901 agttaaattt tgtatattca aaaagctctt gatatttaca gcagagccac catatagaga
     6961 tgcctcagac cctaatgatg aaatttctat atttattcct ttgttaaaag agctaactaa
     7021 catatctttt gtaatctgta atatctcagg gatatcacta attatttggc tatttaaata
     7081 tattatttca ggagcataag ttgtaatagc attatttata gctattgtta agtaactaca
     7141 aaactcatga ataacttttt tggcattatg gttatcttca taatatagtt gctttactat
     7201 atcagaatct attttaggaa tattttctaa agaagaaagc tgttcaaata cctttttttc
     7261 tgaacaatac tgttctaaac agccacgatt tccacaagga catagtttac cgttaggcat
     7321 gatgatagtg tgaccaattt ctccactcat accatttctg ccactatata atttattatt
     7381 tattataatg cctgaaccaa atccactgtg aatactgaga ctaagtagtg agttatgtac
     7441 agttgaaaaa gtattctcag ctaaagctgt taagtttgct tcattttcta tatgaatagg
     7501 aaagtcatac tttttgctaa ggatgctata taagtcaatc tcattaagat tgtaatatgg
     7561 agtaaacaat actttatttt cacaagtaat cccatgtata gccaaagtaa gaccaattac
     7621 cttataagga gtgtctattt tggatatgtt ataactattt ataatttcat ctatcaattg
     7681 tataacattg tctttactta cttgtatatc tgttaatttc ttagaattta taatagttcc
     7741 atctaagtaa gatagagaag aaaatatata gtcgtatcca atgtccatac ttaaagaaat
     7801 ccctgcacat ttattaaata ctaataatat gggttttctt ccgccactat gagtactatt
     7861 tccaattcct atttcatgaa ctagagattc atcaataagt tttttggtaa tagcggatat
     7921 agttgcttta ttcaatccta tagtagaagc tatacttgcc ctagaaatag gaccattttt
     7981 tataatttgt tcaagtacca atctttcatt catttctcga atagtgtatt tatcagtaac
     8041 caatttgata ttcctcctta aaataatatt gtaatacttt ttacacaaaa ataaaaggtt
     8101 attttgcatt gacaaagata attaaatatt ttattattag ttcataagtt agtttaatat
     8161 actaacaaaa ataaagcaag taaaatatac ctaaaatata aaaaaattag gataggaaaa
     8221 cgatagttat gaagtggcat tcaaggaggg atgaagttgg agtatgtatt aggtgtggat
     8281 attggtacat caggtacgaa aacagtttta tttgataaac taggaaatac tataaaaagt
     8341 tgtacatatg aatatcctct tatacaagag aaatcaggtt gggcagagca ggatgcaaat
     8401 gattggtgga aagctgtagt cgaatctata agagaagtag ttcaaagttc aaatataagc
     8461 agtgaatgta taaaaggaat tggattatca gggcagatgc atggtctagt tatgctggac
     8521 aatgaaggta aaacattgag aaactctata atatggtgtg accaacgaac agtaaaagag
     8581 tgtgaggaga taactgattt agtaggtgaa gaaagactga ttgaaataac tgctaatcca
     8641 gcacttacag gatttacagc atcaaagata ttatgggtta gaaacaatga accagatata
     8701 tataaaaata ctaataaaat acttcttcca aaagattata taagatacaa attgacaggt
     8761 gaatatgcta cagaggtatc agatgcaagt ggtatgcagt tgctcgatat aagaaaaaga
     8821 gattggagcg atgaagtact agaaaagtta aatatagata aaaatctttt aggtaaagtt
     8881 tatgaatcac aagagattac aggttatgtg actagagatg tatcaagttt gacagggctt
     8941 aaagaaggaa caatagtggt aggaggagct ggagaccaag cagcaggagc tataggaaat
     9001 ggaatagtta aagatggagt agtttcatct actataggta catcaggtgt agtatttgct
     9061 tatacaaaag agccaaaaat tgataaagaa ggtagagttc atactttctg tcatgcaata
     9121 ccaaatacat ggcatgttat gggagttact caaggagctg gcttgtcatt aaaatggttt
     9181 aaagataatt tctgtcaaag tgaagtggaa gtttcaaatt ctttaggtga agatgtatat
     9241 gagattataa ataatcaagt aagtaaagta ccaacaggat gtaatggatt gctttactta
     9301 ccatatatga tgggagaaag aactcctcat cttgaccctt atgcaagagg tgtgttcttt
     9361 ggattatctc caatccattc taaaaaagaa attgcaaggt caataatgga aggtgtttca
     9421 tatagtctta aagactgtat ggatataata gagaatctta atatagacgt taatgaagta
     9481 agagcctcag gtggaggtgg aaaaagtaaa gtatggagac agatgcaagc agatatgttt
     9541 aatcaagatg tatatactat aaattcaagt gaaggaccag cacttggagt tgcaattctt
     9601 gcattagtag gagctggaat atatgaaaat atacaaaaag cttgtgatgc tataataaaa
     9661 acttcaacta aattagaacc aataagtgaa aatgtagata catacaatat atatcataga
     9721 ttgtataaaa gaatatacaa atctttgaaa gatgacttta agttattaga tgaagtagtt
     9781 aatgcaaaaa taaataatta aattagctta aaaagaaaaa taaagataga aaatagtttt
     9841 aaattaagaa aacatactac atatattata tgtgtaaaat attaggagga catgaaatga
     9901 gtgaaatatt taaaggaata ggacagataa aatttgaagg agtaaaatct gacaatgaat
     9961 tagcatttag atactacaat cctgaacaag tggtaggtaa taagactatg aaagaacact
    10021 taagatttgc aatgagctat tggcatactt tatgtggaga gggtaatgac ccatttggag
    10081 taggcacagt agagagacct tggaataata taacagaccc aattgagata gcaaagatta
    10141 aagttgatgc aggatttgaa tttatgtcta agatgggaat tgagtatttc tgttttcatg
    10201 acagagatat agcaccagaa ggaagagatt tagaagagac aaataagata ttagatgaaa
    10261 tagttgagta tataaaagtt aatatggaaa agacaggtat aaagttatta tggggaactg
    10321 ccaatatgtt tggaaatcca agatttgttc atggtgcatc tacaacatgt aatgcagatg
    10381 tatatgcata tgcagcagct caggttaaaa aagctatgga aataactaag tacttaggag
    10441 gagaaaattt tgtattctgg ggtggaagag aaggttatga aactttactt aacactaata
    10501 cagaacttga aatggataac tttgcaagat tcttacaaat ggctgtagac tatgctaagg
    10561 aaataggatt cactggacaa ttcttaatag agcctaagcc aaaagagcct actaaacatc
    10621 aatacgattt tgatactgct actgttttag gatttttaag aaagtataat ctggataaat
    10681 acttcaaaat gaatatagaa gcaaaccatg caacacttgc aggacatact ttccaacatg
    10741 aattaaatat agcaagaata aacaatgttt taggtagtat agatgcaaat caaggagacc
    10801 tattattagg ttgggataca gaccaatttc caacaaatat atatgatgca acacttgcta
    10861 tgtatgaagt gttaaagcaa ggtggaatag caccaggagg gtttaatttt gactctaaag
    10921 taagaagagc atcttttgag gtagaagact tattcctagc ttatattgca ggaatggata
    10981 catttgcaaa gggattatta atagctcata aattattaga agatgaggta tttgaaaact
    11041 ttactaaaga aagatatgca agttttagtg aaggtatagg taaggatata gtagaaggta
    11101 aggttggatt taaagagtta gaatcttatg cactgcaaat gccagttata aagaataagt
    11161 ctggaagaca agaaatgcta gaagctatac ttaacagata tatatatgaa gtagatacaa
    11221 tttctaataa gtaattgtgt agtaattaaa cttggatgtt tgaataaaat aacaggatat
    11281 gttcttgttt ttattctaaa acggacaaag ataagtattt gtaaaaattc tcataatcat
    11341 gataaattaa ggatatagac ttaagtataa ttagtttata tccttaaaat ttctataaaa
    11401 aataatcatt tctatagtta tcaatatttt taattatcaa tatctttctc aactaataaa
    11461 taaaaatcag taaagtatct aaatttaagt cattataatg gataaatttt ttagcatatc
    11521 ttttttag
//
LOCUS       DAYAPA010000129        11466 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.132, whole genome shotgun sequence.
ACCESSION   DAYAPA010000129 DAYAPA010000000
VERSION     DAYAPA010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11466
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.132"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(113..277)
                     /locus_tag="ACGI59_003180"
     CDS             complement(113..277)
                     /locus_tag="ACGI59_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9740956.1"
                     /translation="MFGKPKESVRFLDFNDVAEKFGEKKAVAIFDAIAGKKSKEHIIK
                     DIEKLVKNEV"
     gene            complement(819..1151)
                     /locus_tag="ACGI59_003181"
     CDS             complement(819..1151)
                     /locus_tag="ACGI59_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFG9740957.1"
                     /translation="MNTREMKEYRGFYNKKLSETDEFFRVFGSLDDKTYSEGAISKKN
                     KELMGLAISILSRCDECICYHIEGCINSGASKDEIIEAIKIGVIGGGSITYPNARFAI
                     KILEEFNI"
     gene            complement(1395..2036)
                     /locus_tag="ACGI59_003182"
     CDS             complement(1395..2036)
                     /locus_tag="ACGI59_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFG9740958.1"
                     /translation="MKKAINKQFILSTLICFIPFIVSIYFYNRLPNEVAIHFDNYGNP
                     DNYAPKVIAAFGVPLLMLCIHLYTWFRLENEPKKANFSNVIQNLSKWGVAILSVIIQI
                     MMVLYSTGVSLESNFYTGLILGIIVILFGNYLPKCKRNYSVGIILPWTLNDEDNWNKT
                     HHLAGWIWLIGGILLIINAFINIPFYNIFVIFVIVILPFIYSYLMYKISGKNN"
     gene            complement(2017..2310)
                     /locus_tag="ACGI59_003183"
     CDS             complement(2017..2310)
                     /locus_tag="ACGI59_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autorepressor SdpR family transcription factor"
                     /protein_id="HFG9740959.1"
                     /translation="MGFPETFKALSDPIRREILVMLRENKMSAGEISEKFNMTNATIS
                     YHLSQLKKAGLIFETKYKNFIFYEINISVFEEVMLWFSQFMEKGEKDNEKSDK"
     gene            2691..2762
                     /locus_tag="ACGI59_003184"
                     /pseudo
     CDS             2691..2762
                     /locus_tag="ACGI59_003184"
                     /inference="COORDINATES: protein motif:HMM:NF024729.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(2933..3067)
                     /locus_tag="ACGI59_003185"
     CDS             complement(2933..3067)
                     /locus_tag="ACGI59_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9740960.1"
                     /translation="MNISRRAMKIIELAQKIANKRGVTVQDAWNDAMKEYKEKYEYVA
                     "
     gene            complement(3278..4030)
                     /locus_tag="ACGI59_003186"
     CDS             complement(3278..4030)
                     /locus_tag="ACGI59_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFG9740961.1"
                     /translation="MFSVIFLCIGGFLAAFVDSIAGGGGLISMPVLMAIGVPVHLAIG
                     TNKFAASAGCISSAYRYAKSGKINNNLLKKLVPFTIIGSVLGVRCVLSISEEILNVLV
                     VVMILIVAIYTFISKNLGQEDNFEAVNKKNLRLGMLMALIMGFYDGFFGPGTGTFLTF
                     GFIKIYGYDFLHASANTKILNLTSNITSLLLFMINGQVDYKIAIVFALVMIMGAYVGA
                     KVAIKKGSKMIKPIFLVMALFMVVKLVYQTLV"
     gene            complement(4089..5744)
                     /locus_tag="ACGI59_003187"
     CDS             complement(4089..5744)
                     /locus_tag="ACGI59_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFG9740962.1"
                     /translation="MEYFSESNNEVQKFDWGEVMWIHEPSKTQFNRLSAGIVRFFPGN
                     YQEKHFHLSEEQLLYVIQGEGIQIIDGKKVNIKETSIVYCPPYSEHEIINTGKIDLVI
                     LITYVPHKFSSLRQIPIVFSENNIQELVNVNIIENLANQISNILKLRISVYDLKYKEI
                     FETKKENKFCDICKNIKQCTKKLVKSINNDNFEKVYHCEYGVSSLEIPIVLEENIVGY
                     IECGNFIVYKSIDIDKNLSELSSSSGIDIKYINKIYNEFPLNPKSRLYVLKENLLMMS
                     EFIQEIAKRNFFENQLSIKDEEILKSRKENIRLEEALKKANSRLLEEEYIINPIDMFN
                     NSSKEYPFELELYIEKEIKNMNLEGVYNLIDTNKLRYNDVRDTIQEMIFVLSRTVLRD
                     LEDLKLISYLRNKYNKKISLVNSDEDLWNVLFEFSKECIDKNRAFWRKDKGNLIENIN
                     EYIKKYYKENINLNSISDVFFISPNYLSSIFNERNKVSITEYINLLRIEESKKYLLDR
                     SMSISDICKKVGFNNSSYFSQIFKKFNSITPNEYRKNMLDNKY"
     gene            complement(7017..8096)
                     /locus_tag="ACGI59_003188"
     CDS             complement(7017..8096)
                     /locus_tag="ACGI59_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFG9740963.1"
                     /translation="MILITLCILVAVFVLAFAGLRLIAPTIIRTSHKIEAPGIDRMEM
                     VEIGGIQQALYIRGQNADNPIILWVHGGPGNSMMPFLHLYQYEWENDFTVVNWDQRNV
                     GKTYFANDPAAVLQTMSAERVLQDVHEVTAYLKQEFNKEKIILIGHSWGTVLGTMAVQ
                     TYPEDYSAYIGIAQTVNMVDNERVGYEKTLEMVKAAGKQKDIKALEAIAPYPPAKYGP
                     SYLAALMTVRTYQSKYGLSINMDLGNIISILATPYYSLRELKNTAIFNPNAIDNQGDV
                     LRFLVEEADIRNYGVDYQVPVYYIMGENDYNSPYLPAKKFFDEIVAPDKQIFFIPDAD
                     HLPFLSKKAEFDCVLLEEIAPKLAK"
     gene            complement(8180..8836)
                     /locus_tag="ACGI59_003189"
     CDS             complement(8180..8836)
                     /locus_tag="ACGI59_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFG9740964.1"
                     /translation="MTRKNNPKQAIENIITISAKLFAEKGYDKTSMQNIVDASGMSKG
                     GIFHHFSSKEDIFNVVMERRFEQIIETVNQWLGEMHGLTAKEKLRSLVRRHLTDEVIK
                     ESSNMITSAIESPHIILAFTQDNLKKLAPILANVLREGIEDRSIITEFPDECAEVILL
                     LFNFWCDTDVFQGDFPTLRKRFQFLQFLMRQIGVDILEDEIIESINIFYERKPMGQVE
                     "
     gene            9158..9364
                     /locus_tag="ACGI59_003190"
     CDS             9158..9364
                     /locus_tag="ACGI59_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9740965.1"
                     /translation="MISHAAEICKKYKMPFIIFPTIEKKSIEEWVNIGANMLCLAQDT
                     TFITYVINQLLSFYNEIVPIQKQH"
     gene            complement(9517..9945)
                     /locus_tag="ACGI59_003191"
     CDS             complement(9517..9945)
                     /locus_tag="ACGI59_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFG9740966.1"
                     /translation="MSKIYIVYWSGTGNTEKMANFVAEGVKLKGKTPEVLDVSLLKPS
                     DLKEEDKFALGCPSMGAEQLEEGYMEPFVSELESMVSGKQIGLFGSYGWGNCEWMRDW
                     EERMQNAGATIIGGEGITAMEDPNEEAKDECIELGKTLAE"
     gene            complement(10421..11359)
                     /locus_tag="ACGI59_003192"
     CDS             complement(10421..11359)
                     /locus_tag="ACGI59_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HFG9740967.1"
                     /translation="MNKRMKLIPYEINENLRGAKNKFPYGIKQMNARGMWDEGYTGKN
                     IVVGIIDTGCDISHPLLKGKIIGGANFSDDSNGNKNIYEDFNGHGTHVAGIIAASNYN
                     NEVMGVAPDCKLLIAKALNKDGTGTYQSIINAINFAVNNKVDIISMSLGGNKDDKNLK
                     NAVMQAVKNNISVVCAAGNNGDGDSSTSEYSYPASYAEVIEVGAINENYLVEKFSNSN
                     TTIDLVAPGRNIISTYMDNKLAIMSGTSMSAPYVSGSLALIKEWAREEFERDLDEAEL
                     YAQLIKCTRALGIPRTEQGNGYLYLNLYKYKNNSKR"
BASE COUNT         3860 a         2013 c         1180 g         4413 t
ORIGIN      
        1 aatattactc tataacaata agtttcttat ttaaatttta aaatattatc taaaaatcat
       61 aaaaaagaag gtataaagag ctaccctcaa gctcaatata ccttataaat atctaaactt
      121 cattttttac taatttttct atatctttga taatatgttc tttagatttt tttccagcta
      181 tggcatcaaa aattgcaaca gcctttttct ctccaaactt ttctgctaca tcattaaagt
      241 ctaaaaatct aacactttct tttggtttac caaacatata acacctctca tacattttta
      301 tatatttatt ataacttcta tagacttatt tttcaccaat aaacgttaga caaattagtc
      361 cctttatttt tttaaactag ctttaccttc aaaattctta acaaaaacag tttattctac
      421 tctataaata aaaatagaca atttaatttg taggaaactg ataatagaat acagtaaata
      481 aaactttgtt taatctatct gaaaaagtta tctattaaaa ctgctatgaa tttataaaga
      541 atattctagt tcataaaaat acaaatatgt aaatataatc accttaatat tcaatttatt
      601 taaaataaaa aatgtataaa agatttaata atgacaatat actattctca atacaatcta
      661 tgatgtaaat aatttaaaac tactatatta tttaaaaatt tatatttata tatgcaattc
      721 taaaaactta ataagaagtg aacttagaaa ataaagctta atttcaaaga cttaaataga
      781 ctattattat tagtctaaat aaactacgtc tatcaaaatt atatattaaa ttcttctaaa
      841 atttttattg caaatcttgc gtttggatat gtaatagacc cgccaccaat tacacctatt
      901 tttatagctt ctataatttc atctttactt gctccagaat taatacaacc ctcaatatga
      961 taacaaatac actcatcaca tcggcttaaa attgaaatag ctagtcccat taattcctta
     1021 ttttttttgc ttatagcccc ttctgaataa gtcttatcat caagacttcc aaatactcta
     1081 aaaaactcat ctgtttcgct taatttttta ttgtaaaatc ccctatattc tttcatttct
     1141 ctagtattca tcttctacct cctatgtttt tcttataaga atatcacacc gtacttatat
     1201 tttcaatatt aaatacaaat tttcatttta tgttcattag cctaatttat attatttttt
     1261 gaaatctcaa taagttatca tataagtaag attttttcag aactaaaata gaaatcttag
     1321 taatttcttt attatctatc tcagttctga aatatattat tcttatccaa acattattat
     1381 agtgattaat tatattagtt atttttacct gaaattttat acatcaaata agaatatata
     1441 aatggtagta ttacaattac aaagattaca aagatattat aaaaaggaat attaataaat
     1501 gcattaataa ttaataatat tccaccaata agccaaatcc atccagctaa atggtgtgtc
     1561 ttattccaat tatcttcatc attaagagtc catggtaaaa ttattcctac tgagtaattt
     1621 cgtttacatt taggaagata gtttccaaat aaaataacta ttattcctaa aattaatcct
     1681 gtataaaaat tagactccaa actaactcct gttgaatata aaaccatcat tatttgtata
     1741 attactgaaa gtatagcaac tccccatttt gataaatttt gtataacatt tgaaaaatta
     1801 gctttcttag gctcattctc aagtctaaac catgtataca aatggataca tagcattaac
     1861 aatggtacac caaaagctgc tataaccttt ggagcataat tatctggatt tccataatta
     1921 tcaaaatgaa ttgcaacttc atttggtaat ctattataaa aatatattga tacaataaat
     1981 ggtataaaac atattaaagt agataaaata aattgtttat ttatcgcttt tttcattatc
     2041 tttttctccc ttctccataa attgtgaaaa ccaaagcatg acctcttcga aaactgaaat
     2101 atttatttcg tagaaaataa agttcttata ctttgtttca aatatgagtc ctgccttttt
     2161 tagttgagat agatgataag aaattgtagc attcgtcata ttaaattttt cactaatttc
     2221 tcctgctgac attttattct ccctcaacat aaccaatatt tctcttctta tagggtctga
     2281 aagagctttg aatgtttctg gaaaccccaa caccatcacc tcgctattta gaatttcatc
     2341 taaatagaat ataacataac atccatttta tttcaatagc tatttagaaa tttttctaaa
     2401 tagttatttc taaatattta ttgaaaattt ttatattaag attctcaatt tacttaagtc
     2461 gcttttattt aatcttactt tatctaattt ttttaactta acaaaagtgt attgcaccat
     2521 aattgttaaa gcaaaaacct aatgattgag gtacttatat atataacaat acttttaatt
     2581 ttaaattaaa agtagctaaa cccaatttaa aaagtagtta agtcctattt aaatagaaaa
     2641 gaatgaaacg agaaatatac tgtataaaag tacataaaaa catctactaa ttagatacaa
     2701 ctataacaga atatatttat tattataata acaaaagaat aaaaaaaatt aatttagcat
     2761 aaccttgtta aatatagatt tgaatatgaa tttaaagtta tgtaaaatta aaaatacagg
     2821 aatgaaaatt ccattcctgt ataaaatcta acttttgagt ctcagtacaa tcttattttg
     2881 agacaatctt ttttgtttta tattttctta tatttaaaat ataacgcata tcttatgcaa
     2941 catattcata tttttcttta tattctttca tcgcatcatt ccaagcgtct tgtactgtta
     3001 cacctctttt attagcaatt ttttgtgcca gttcaattat cttcatagct cttcttgata
     3061 tgttcataaa cattcctcct acaaattacc atatatttca tatatataaa tcaaggttat
     3121 atttttatga tatataatcc ttctattgta agattacagt ttatatgtgt attatatatg
     3181 aactatatta gaagataatt agaattttta taaagctata tgattagatt aaatataaaa
     3241 gctttacaat tacttataaa tttaaaaagt ctataagcta aactaatgtt tgatatacca
     3301 attttacgac cataaataat gccattacta aaaatattgg ttttatcatc tttgaacctt
     3361 tctttatagc aactttagca cctacataag ctcccattat catcactaat gcaaacacta
     3421 tagcaatttt ataatctact tgaccattta tcatgaataa taacaatgaa gttatattac
     3481 ttgttaagtt taatattttt gtatttgcag aagcatgtaa aaaatcatat ccatatattt
     3541 ttataaatcc aaaagtcaaa aaagttcctg tacctggacc aaaaaaacca tcataaaaac
     3601 ccattataag agccataagc atccctaatc ttaaattttt cttattaaca gcttcaaaat
     3661 tatcttcttg acctaaattt ttagaaataa aggtgtaaat agccactatt aaaatcataa
     3721 ccacaaccaa aacatttaat atctcttcac ttattgataa tacacatctc acacctagaa
     3781 cactaccaat aatagtaaat ggtactaatt tttttaaaag gttgttatta atttttccag
     3841 actttgcata tctataggca ctacttatac atcctgctga tgctgcaaat ttatttgtac
     3901 caatggcaag atgtactggc actccaattg ccataagaac aggcatgctt ataagtcctc
     3961 ctcctcctgc tatagaatct acaaaagcag ctaaaaaccc ccctatacat aaaaaaataa
     4021 ctgaaaacat aaaattcctc catcattctc tttcaatttt tcttacaata tatattatat
     4081 aatcttattt aatacttatt atccagcata ttctttctat attcatttgg agttatacta
     4141 ttaaacttct taaatatttg tgaaaaataa ctactgttat taaaacctac tttcttacaa
     4201 atatcactaa tactcatgct tctatctaac agatattttt tagattcttc tatcctaaga
     4261 agatttatat actcagttat tgaaacttta tttctttcat taaaaataga actcaaataa
     4321 tttggactta taaaaaaaac atctgatata ctattcaaat ttatattttc tttataatat
     4381 ttttttatat attcgtttat attttcaatt agatttcctt tatcttttct ccaaaatgct
     4441 ctattcttat caatacactc tttggaaaat tcaaataata cattccataa atcttcatca
     4501 ctgtttacaa gtgatatttt tttattatat ttatttctta aataagatat aagttttaaa
     4561 tcctctaaat ctctaagcac tgttctagac aatacaaata tcatttcctg aatagtatct
     4621 ctaacatcat tatatcttaa tttgtttgta tctattagat tataaacacc ttctaaattc
     4681 atatttttaa tttctttttc aatataaagt tctaattcaa agggatattc ttttgaagaa
     4741 ttattgaaca tatctatcgg attaataata tactcttctt ctaataatct actatttgct
     4801 tttttaagtg cctcttcaag tcttatattt tcttttctac tcttcaatat ctcctcatct
     4861 tttatactaa gttgattttc aaaaaaattc ctcttagcta tttcctgaat aaattcagac
     4921 atcattaata aattttcttt taatacatat aatctacttt taggatttaa tggaaattca
     4981 ttgtaaattt tgtttatgta ttttatatca ataccagaac tacttgataa ttcagataaa
     5041 tttttatcta tatcaataga cttatatact ataaaattac cacattctat atatccaact
     5101 atattttctt ctaatactat aggtatctct aaactggaaa caccatactc acaatggtac
     5161 actttttcaa agttatcatt atttatgctt ttaacaagtt ttttagtaca ctgttttata
     5221 tttttacaaa tatcgcaaaa tttattttct ttcttagtct caaatatttc tttatacttt
     5281 aagtcatata cagatattct aagcttcaat atattagata tttgatttgc aagattttca
     5341 attatgttta cattaactaa ctcctgtata ttgttttcag aaaatactat aggtatttgt
     5401 ctcaaactcg aaaatttatg aggaacataa gtaattaata ttactaaatc aattttacca
     5461 gtgtttataa tttcatgttc tgaatatggt gggcaataaa ctatagatgt ttcctttata
     5521 ttgacctttt taccatcaat tatttgtatt ccttctccct gaatcacata taaaagctgt
     5581 tcttcactta aatgaaaatg cttttcttgg tagtttcccg gaaaaaatct tacaattcca
     5641 gcactaagtc tattgaattg tgtttttgat ggttcgtgta tccacataac ctctccccaa
     5701 tcaaattttt gtacttcatt atttgactca ctaaaatact ccataagctc acctcttagt
     5761 ttttgtctaa atattttttc ttcccttctt atattaaaat aatatcatca tcattaactt
     5821 tcattactat aaaatcatca tactctttta atactcctgt aattttagtt gcacttagtt
     5881 tactacaatt cagtttttta atatattgat aaattctata attacttttt tattttgtaa
     5941 aatcagtgtt ttcccaaaat aattatatct ataaatgaaa gtatttttac tgcttacatc
     6001 aatatatgtt atcataattt tcctttttat tgcatatata aataactgta tataacattg
     6061 tccaatacta tatagaaata catctaagaa agaatattat taaattgttc caattagtat
     6121 tatttttctt atagaaataa aaaaactgtt ggagtctttt agaaaaatta ttttttacat
     6181 accttttttg cttagatagt ctatcttcac agctattttg tatatacact cttctagttc
     6241 atgattttat tttaatatct tgcttatatt gctgaaataa ttgaattctt ttatctatca
     6301 tattgtctct tatatcacaa acatattgtt tcttatatca caaaaatgaa agcattctta
     6361 tatcttcaat atataaacct gcttttttta atcttgtaat aaatatagga tactctatat
     6421 tctcttccta gaaataacta ataccactta tttttctatt atatagaaag taataaatct
     6481 ttttcttaat aaaattcaat agtaagtgga ataaaatttt atatgaataa gaacattata
     6541 aaaatacttt atacaatagt caataaaaga tattagcttc aaattatgtt aaagttgtat
     6601 tatttaaagt gaaaaaacaa ataaaaaaga aataatagta taagtagaaa aatatactta
     6661 aaacaaggcg aagcctctct tgtatctaaa tcaagtagag aattaaatct aaaaaagaaa
     6721 ataaatataa aagatgactc ctgtacatta caagaatcat cttatggttt cataatcact
     6781 ccatggtttt taactgtctg atataaaggt ctcagttcac tcatagcatg accacctttt
     6841 ccgattgctg accccgtcac ttatggacaa aatgtccata agtgatagtc ctctcttaaa
     6901 accctctaaa tagatatatc tcaagcaccg cattgaaacc agttgctgga aacaaactga
     6961 agcgttctgg aatagtaaaa gaaattgcca ccatatccac gttactgtcc tagttgttac
     7021 tttgctaact tcggagcgat ttcctctaac aatacgcagt caaattctgc ctttttgcta
     7081 agaaatggca gatggtctgc atctggtata aaaaaaatct gtttgtctgg cgccactatt
     7141 tcatcaaaaa atttcttcgc tggaagatag gggctattat agtcattttc tcccatgatg
     7201 tagtatacag ggacctgata gtcaactcca taatttctta tgtcagcttc ttccactaaa
     7261 aaccgcaata catcaccttg attgtcaatc gcatttggat tgaaaatggc cgtatttttt
     7321 aactctctca atgaataata cggggttgcc agtatactga ttatattgcc caaatccata
     7381 tttatagaaa gcccatactt gctttggtat gttcttactg tcataagagc tgccaaatag
     7441 cttggaccat attttgctgg cggatatggt gcaatagctt ccagcgcctt gatatctttt
     7501 tgcttccctg cagctttcac catttcaagt gtcttttcat agcccacgcg ctcattgtcc
     7561 accatattaa cggtctgcgc aatcccaatg tacgcgctgt aatcctccgg ataggtttgc
     7621 acagccatag ttcccagaac tgttccccag ctatgaccta taaggataat tttctccttg
     7681 ttgaactctt gcttcaaata tgctgtcacc tcatggacgt cttgcagaac acgctccgcg
     7741 ctcatggtct gcaaaactgc tgccggatcg ttcgcaaagt atgttttgcc tacgtttcgc
     7801 tgatcccagt tcaccacagt aaaatcattt tcccactcat actggtacag atgtaaaaat
     7861 ggcatcattg aattaccagg acccccatga acccaaagga tgatggggtt gtctgcgttt
     7921 tgtccccgga tatataacgc ctgctgtatg ccgccgatct ccaccatttc catgcggtcg
     7981 attccaggag cttctatttt atggcttgta cgtataatcg tgggggctat cagacgcaaa
     8041 ccggcaaacg ccagaacaaa aacagcaact agtatgcaaa gtgtgattaa tatcatttta
     8101 agtaccttct ttcatgaatt tttttagcca tagatatctc ctctaacatt aaaaaaaata
     8161 tgttgctatt ttagttttgt tactctacct gtcccattgg tttcctttca taaaaaatat
     8221 taattgattc tataatctca tcttccagga tatcaactcc tatttgtctc ataagaaact
     8281 gtaagaattg aaaccgttta cgaagtgtag gaaagtcccc ctgaaataca tctgtatcac
     8341 accaaaagtt aaagagcagc aaaattacct ctgcgcattc atctgggaac tctgtgatga
     8401 tgcttctatc ctctatgcct tctcgtaaaa catttgctaa tattggtgct agttttttca
     8461 aattatcctg tgtaaatgcc agtatgatat gcggactttc tatcgcgctt gtaatcatgt
     8521 tactagattc ttttatcacc tcatccgtta aatgccgcct aactaaactt ctaagcttct
     8581 cttttgctgt aagtccatgc atttcaccga gccattgatt tactgtctct attatctgtt
     8641 caaagcgcct ttccatgacc acattaaaaa tatcttcttt tgaactgaag tgatgaaaaa
     8701 ttccgccctt tgacattcct gaagcatcaa caatattttg catacttgtt ttgtcatatc
     8761 ctttttcagc aaacaacttt gcagaaatgg taatgatatt ttcaatagct tgctttggat
     8821 tgttctttct agtcattata taactccttc ttattaaaaa ccgactgttg gtttttatta
     8881 tataagctat tcctgcttct gtcaagtaac attgtgttta tggcaaaaca ataaggtttg
     8941 gtggttagac aggtattaat tagactaatg cgactttccc ctagatggaa cccttggtaa
     9001 atttgcaatc tttaccggct ttgattatat ctgtaagcaa ggcaggtatg gccacacctg
     9061 ccctgtttgc ccctcggata ttcattccta tccctcctct ccttagccat tacaccaaaa
     9121 cgccgctcat tgtctccaat ggcggcgttt taaagatatg atttctcatg ctgctgaaat
     9181 atgcaaaaaa tataagatgc cctttattat ttttcctaca atagaaaaga aaagtattga
     9241 agaatgggtt aatattggag caaatatgct ttgtttggca caagatacta cctttatcac
     9301 ttatgttatc aatcaattat tatcttttta taatgagatt gtaccaattc aaaaacaaca
     9361 ttaaattata aatctaattt tatattaaaa gagactaata ttttatctat ctctattaag
     9421 gatattttct tatgtatcat aatattagtc tctcttatta ttatatactg ctatcatcat
     9481 tagttgctat ttatactatt tttataatat acaaatttat tcagctaacg ttttgcctaa
     9541 ttctatacac tcatcttttg cttcttcatt tgggtcttcc attgctgtaa ttccttctcc
     9601 accaataatt gtagcaccag cattttgcat acgttcttcc cagtctctca tccattcaca
     9661 atttccccat ccatatgaac caaataatcc aatctgttta cctgatacca tagattctaa
     9721 ttctgaaaca aatggctcca tatacccctc ttctagttgc tctgctccca tagatgggca
     9781 acctaatgca aatttatctt cttcttttaa atcacttggt ttcagtaagc tcacatctaa
     9841 aacttctggt gtcttacctt ttaacttcac accttcagcc acaaaatttg ccattttttc
     9901 agtatttcct gttccactcc aataaacaat atatatttta ctcattttta cctcctgtag
     9961 tattttttaa ttaatcaata gttagttata gctaactaac atctaaaaaa caaaagattt
    10021 ctttatctga aatctcttta tttttctaaa tatagaatat catggttgat tttgaaattc
    10081 aatacttatt gataatattt atcatttata ttttatattc attttataat atcaaaacac
    10141 tgtatcgtat ttctaatctt tcatagaaaa atttctaact tatagtactt ttgcatctct
    10201 tataaatctt ttatatgtat aagtacatag aaaaacacac cttttccaga tgtgctatct
    10261 ataatatcgt atatttattt ttcaattcta actagctaat atgtttataa attttatcac
    10321 ttaatacctt tatacattga tgtaaaatct tacacctaag taatttttat tatttgtctc
    10381 gactctatat aatatagcta ttttatcatc aaatcaaaaa ttatcttttg ctattattct
    10441 tgtatttata aagatttaaa tataagtatc catttccttg ttccgttcta ggtattccaa
    10501 gcgctctcgt acattttatt aattgtgcat acagttcagc ttcatctaaa tctctttcaa
    10561 actcctctct tgcccattct ttaattagtg ctaatgagcc tgatacgtat ggtgcactca
    10621 tactggtacc actcataata gcaagtttat tatccatata agtcgatata atatttcttc
    10681 ctggagctac caaatctatt gtagtatttg aattactaaa cttttcaacc aaatagttct
    10741 catttattgc acctacttct attacctcag cataactggc tggataacta tactcacttg
    10801 tactagaatc accatctcca ttattacctg ctgcacacac tacagaaata ttatttttta
    10861 ctgcttgcat gacagcattt tttaaattct tatcatcttt gtttccccca agagacatag
    10921 atataatatc aaccttgtta tttacagcaa agttaatagc attaattata ctttgatatg
    10981 ttccagtacc atctttattt aatgcttttg ctattaataa tttacaatct ggagctactc
    11041 ccataacttc attattataa ttagatgcag ctataatacc cgccacatga gttccatgac
    11101 cattaaaatc ctcatatata tttttatttc cattactgtc atcactaaaa tttgcaccac
    11161 caattatttt tccttttaaa agaggatgag atatatcaca acctgtatct attataccaa
    11221 ctacaatatt tttaccagta taaccttcat cccacattcc cctagcattc atttgtttta
    11281 ttccatatgg gaatttattt tttgcacctc ttagattttc atttatttca tatggaatta
    11341 gtttcattct tttattcatc atatatcctc ctaaaaattt tattgcttat aaaaatcatg
    11401 aattttattt tataataggt ttataaatca aattaaaacc tatatctact tatttatatt
    11461 tatgat
//
LOCUS       DAYAPA010000130        11387 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.133, whole genome shotgun sequence.
ACCESSION   DAYAPA010000130 DAYAPA010000000
VERSION     DAYAPA010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11387
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.133"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            155..574
                     /locus_tag="ACGI59_003193"
     CDS             155..574
                     /locus_tag="ACGI59_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9740968.1"
                     /translation="MQIYFRVKAVGKRKPMLELTPFDVSDNINCLKEAISEIVSKNVL
                     EYNEKIPEKSIINFLTNEEIESQADIGKVGFGSIYNENKQSVEKAIEVALQAFEDGIY
                     KVLINEDVIEKLDSSINLKSGDIFTFIRLTLLAGRMF"
     gene            599..5656
                     /locus_tag="ACGI59_003194"
     CDS             599..5656
                     /locus_tag="ACGI59_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HFG9740969.1"
                     /translation="MLERTRVEKNPKIIENFKNNFTKSSSKQYYDLAKSLWDCIENSS
                     YYYREPEDKFLNMIKKYYNCHYYEIKNMDELLSGPLKNALELIFTSEQVEELCQLIKR
                     KMDYTYSGGYYRKGYRSNKLVDYFRGIIRFISNYVDLIYYDFSVIDYLTLPNKVLYYD
                     SIMSDKIALEIDKGNQKVISALEEVIFGENQTALLTRQMIIGIMKSHSDKCYEMLSKL
                     LLAAKQQEGLRQQIVESLDEGTISASIYMLKTILDNNLERFSSVIRALDTWTGLGFSD
                     QKPGIIRKCLELSYKSLTDEKFRIESINSNDNLEIYFALWATATYNIYDSYSLIENLL
                     SCQEKYKKLVALYFLSHTESTYFKHSIASKNIKGDDLEILAWVITNFYVEGSVGYYYS
                     WQNRETNFDLSNTPIDKDFDARKEQFESLKHTLEILNSKTKSFSGSVFPWTWIEISSH
                     EILKCMMTLTAYDMNAQFIDVLMENCHLMSSDLRASLIVNFVNEPKTDRQREFLLECL
                     SDKSVSNRETVLSLLRKLELTDVELDKITNLLSLKTSSIRQTVIKILLEQDDSNLENI
                     ASSLIKSSNSQKCLGGLEIVNLLKDNTARQELYNKLILLIEDIDKSKKNTSQVQILID
                     NLLGKAKNDYCKENGFGIYNPENKVQIPEQFDFKDDSNSSSKLNKIISNISLSTREDS
                     VSVATKKEAKKILDTDINKIIKVYEKFDELITENADYEYEVERWSGNVDKVLLGYSTY
                     NLTPIKYKVSNDTLDNYPLAELWRETSKKLDLDYKLILDILFYSISTCGYNETEKSWF
                     SSIYSNVLDSKKQNKIYTKLEKLKFNGLTRKLLRVLLNEFDHKEIFNYCLEIYYKLRN
                     LLTIEQCKKQRVEFTKNYYRQNEYCPALNAPILNYWLDKAHSFIYDDISFTKFFYTTF
                     SNYQLLEYKFPFYTSINVFARAHELGLMTEEQVNREMISGYNASNIIRTLTNPRWAGD
                     TLEKYPWLKDNLKTVTNRIIEIESRRGDMPTEVSNLANNIQYFEGINYFVNILVALGK
                     ETFQRGYSYGSNYSKKDVLSSLLKNCYPTKEDDSKKLKSLLKNTDITELRLIESAMYA
                     PQWVNIIEKYLNWKGLKSTIWYFHAHINEGFSAQKESEVALFSPISPRSFIDGAFDVD
                     WFLDSYKTIGEERFNILYKSAKYITSGGNSHRRAQLYADATLGKLDKDMLMKEIETNR
                     NQDKLRSFSLLPMKKGDLKEATFRYEFIQKFAKESKQFGAQRRESESKACTIALENLA
                     RSAGFGDVNRMMWALETEKLNEVAHLFKPKDIDGTSIWIDVDENGKASTKVQKGNKSL
                     KSIPKAIAKHPYVLELKSVQKDLKSQYSRAKQSLEQAMKNEIVFTVKEIANIYKNPVI
                     CPLIKNLVWVCDDNIGYVYNDKSKFFICNIEGETYKLTAKNKLKLAHPVHLFESGKWS
                     EFQHDLFSKQIVQPFKQVFREYYRINSDELSEKTISRRYAGHQIQPKKTVALLRNQLW
                     TVDYEEGLQKVFYKENIVAKMFALADWFSPADIEPPTIETVEFFDRKTYKSLELASIP
                     PILFSEVMRDIDLVVSVAHAGGVDPETSHSTVEMRIAIAKELLLLLKINNVNFEGSHA
                     LIAGNLGEYSVHMGSGIVHKKGMGALNILPIHSQSRGKIFLPFADEDPKTAEIMSKIL
                     LLAEDSKIKDPSILSNL"
     gene            complement(5841..6578)
                     /locus_tag="ACGI59_003195"
     CDS             complement(5841..6578)
                     /locus_tag="ACGI59_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFG9740970.1"
                     /translation="MIKVLICDDDKIVRDDIANIAKESGYVDYVKKVKDGIEAIEFIK
                     NEKIDLLIIDIDMPYKNGIDSAEEILSIDKDIHIVFVTGFADYSLESFKVHPIDFVVK
                     PFKKEKILDSINIAIDHINSHKIANNNFIEDTLFVYKIRKQIHMINFDDIVMFEKNSR
                     AINLHTKDEDIIKFYENFDDLKKRLPPNFFMTHRQYIVNLRFIHKVIPINKSSLEIRF
                     INFQENAILNKSLEKDFLYRFYRVKRF"
     gene            complement(6597..7532)
                     /locus_tag="ACGI59_003196"
     CDS             complement(6597..7532)
                     /locus_tag="ACGI59_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFG9740971.1"
                     /translation="MDNNFVFPIVILTLFIFSNLIMNKYKFYKELDILKRGILTFFIS
                     CFALILFMEGTKYIQLRLGYSFLLEGVLNIILFLVLIILSFYTGFKVIRLINNNNEQE
                     YKLQKLDSKINHKDNIIIKLRSQKHDYLKHLQVVYSLLNTGLDEDAKEYIMNISNSFK
                     SKNSRYGKVSYLDAIASLKYEECLEKDISFDVYIQDFMDNLEIEPNDMSSVLLNIIDN
                     AIESLSTIKKEKKYIRLHAYEDEFQYIIAIKNNGPKIHNVESIFWNGFSTKEGQDRGY
                     GLHLVKNILDKYDYSILVNSDDFLTEFVILIPKYY"
     gene            7740..10316
                     /locus_tag="ACGI59_003197"
     CDS             7740..10316
                     /locus_tag="ACGI59_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9740972.1"
                     /translation="MKDNLKIVLRYIKSYKARSLAIVVSIILATSLVVGIGTLSRSAQ
                     QADVDRLKRELGTDHVYFKNIKKNQLKSIKSSKDIKNLGITSYYASTDVNEKLPINIL
                     YTNKNYLTSHSKLIKGRFPKASNEVVVEKWILNSMGLKPEINQNLTFKLYQKENPETF
                     KVVGILEDRFIEKKKGQCEIFSNLNESELNDFTTYIEFNEGSDINTNIDNIIKNAMLD
                     EKSIGRNSMLIESTMKNGTLDNSSKYVAIAMSLFAGIVIYSIYVISIYQRVQEYGVLR
                     AIGSTNFRIFKLMLYELFILALIAMPIGICIGMGGAQIFNRSVGNIQFEGNINVTPFV
                     IPDKIILLSIACTILIILIISFFTYLKIRRISPIDAIRKTFGADKNVTKVNYLISKLT
                     LNMSVTKSISAKNIFRNKKGFIIIVLSMSIGGIIVIKDNYKYSFLDVQNKSTQEQTYM
                     NADFILSNVYLNEIGNNTSFKDIKGFNDNQIDKIKNIDGIDKVKTASILNTRIEVNKI
                     NRLDYYEAINSTPYYKLNPLFIKNKTTGKYTLKQKLKGYNDEMIKSLEKYLVSGNINL
                     KKMKKENLAIVYVPEISKTQKYTKTFTPGEGTPAVDIKVGDTIKVKYPKGGMDTGLYE
                     SLKDNYEYLEYEFKVGAIISYPFADNGMYSGDDGVDVITSSEYLKKLTGVDKYNLVYA
                     DLNENANKKKINNLLGKIGSEVPGTTTVDMMVEKENSDKMTSRTMIYSYGIVAIMFII
                     SVLNIINNISYNLTSRTSEFGMLRAIGISERGFKNMILYEGILYGVLSSIITIVSGII
                     IQFKMYYMQGFVSYGLGFSIDYKIYILVVVANIIVGILATYIPSRKINKISIVEAINI
                     TE"
     gene            10328..10999
                     /locus_tag="ACGI59_003198"
     CDS             10328..10999
                     /locus_tag="ACGI59_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9740973.1"
                     /translation="MPILETINLGKIYGKKETSVHALKNANLKINKGEFVAIIGPSGS
                     GKSTFLHLVGGLERPSNGTIKVDGKDICCLSDKELARYRRQKVGFVFQQYNLIPVLNV
                     KENIELPLKLDNKKIDKEYIDDLINLLGLNERKNHLPNQLSGGQQQRVAIARALSAKP
                     SIILADEPTGNLDSKTTEEVMDLLKSSIKKYNQTLIIITHNENIARKADRIISIIDGE
                     LKLTL"
     gene            11026..11367
                     /locus_tag="ACGI59_003199"
     CDS             11026..11367
                     /locus_tag="ACGI59_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9740974.1"
                     /translation="MLLILGNLNLNLDFDYRIIREENEDVDIFIDINYRSLDIDTDGS
                     NLFNSRIQFPFVRALILRLNKNNQCMTIHLLRDIDLFSAFANFEVDYTDSIINIKNQN
                     EKVILNKSIKK"
BASE COUNT         4430 a         1467 c         1675 g         3815 t
ORIGIN      
        1 ttattgcttt tttagacttt aagatagctt tttttctatt ttttgtaaaa taattccata
       61 attcacatta ataattcaca aaatgatata atataacaaa tagataaatt tttaaatttc
      121 taaatacata attaatattt tgcgaggtga ttttatgcaa atttatttta gagttaaggc
      181 tgttggcaaa cgcaaaccta tgctagaact tacccctttt gatgtatctg acaacatcaa
      241 ttgcctaaaa gaagctatct ctgaaattgt atctaaaaat gttcttgaat ataatgagaa
      301 aatcccagaa aaatcaataa taaacttctt aactaatgaa gaaattgaat ctcaagctga
      361 tattggaaaa gttggatttg gctctattta caatgagaat aaacaatctg tagaaaaggc
      421 aatagaggta gcgttacagg catttgaaga tggaatctac aaagtgctta taaatgagga
      481 tgttattgaa aaactcgatt cctctataaa tctgaaaagt ggagatatat ttacttttat
      541 aagacttacc ttgcttgctg gaagaatgtt ttaaaataaa taatttggag gtacataaat
      601 gttagaacgt actagagtag aaaaaaatcc taaaataatt gaaaatttta aaaataattt
      661 tactaaatct tcttcaaaac agtactacga cttagcaaag tctctttggg actgcataga
      721 aaatagttca tactattata gagaacctga agacaaattc ttaaatatga taaagaaata
      781 ctacaattgc cactactacg aaattaaaaa tatggacgag ctcctttctg gacctttaaa
      841 aaatgcatta gaattaatat ttacttctga acaagtagaa gaactttgcc aattaattaa
      901 aagaaagatg gattatacat attcaggagg atattatcgt aaagggtatc gctcaaataa
      961 acttgtggat tattttagag gtattataag gtttatttca aactatgttg atttaattta
     1021 ttatgatttt tcagttatag attatcttac tcttccaaat aaggtcttgt actatgactc
     1081 tataatgagt gataaaatag cactagaaat agacaaagga aatcaaaaag taataagtgc
     1141 tttagaagaa gtaatatttg gagaaaatca aactgctcta cttacaagac aaatgataat
     1201 tggtatcatg aagagtcata gtgataaatg ctatgaaatg ctttctaaac ttcttttggc
     1261 agccaaacaa caagaaggac ttagacagca aattgtagag agtttagatg agggtacaat
     1321 ttctgcatca atatacatgt taaaaactat tttggacaac aatttagagc gatttagttc
     1381 tgttataaga gccttggata catggacagg acttggtttt tcagaccaaa aacctggaat
     1441 aatacgtaaa tgtttagaac tttcatataa atcattgact gatgaaaagt tccgtataga
     1501 atcaattaat agcaatgata atcttgaaat ttattttgcc ttatgggcta ctgcaactta
     1561 caacatatat gactcttact ctcttattga aaacttactt tcttgtcagg aaaaatataa
     1621 aaaacttgta gctctttact ttttatcgca tacagaaagt acatatttta aacattctat
     1681 agcatctaaa aacattaagg gtgatgattt agagatacta gcatgggtaa ttacaaattt
     1741 ctatgtagaa ggttctgttg gctactacta tagttggcaa aacagagaaa ctaattttga
     1801 cttgtcaaac actcctattg acaaagactt tgatgctaga aaagaacaat ttgaaagttt
     1861 aaaacatact ttagaaattt taaattcaaa gacaaaatca ttttcaggaa gtgtatttcc
     1921 atggacttgg attgaaattt cttctcatga aatacttaaa tgtatgatga ctttaacagc
     1981 atacgatatg aatgctcaat ttatagatgt gcttatggaa aattgtcatc ttatgagttc
     2041 agatttaaga gcttcactta tagtcaattt tgtaaatgaa ccaaagacag atagacaacg
     2101 tgaattttta ttggaatgtt taagtgataa gagtgtttcc aatagagaaa cggtattgag
     2161 tctacttaga aagcttgagc taacagatgt agaattagat aagataacca atctattgtc
     2221 tttaaagaca agctctatta gacaaacagt aataaaaatt ttacttgaac aagatgattc
     2281 aaatttggaa aatattgctt cttcactgat aaaatcttca aattctcaaa aatgccttgg
     2341 aggtcttgaa attgtaaact tattaaaaga caatactgct agacaagaac tatataataa
     2401 gcttatttta cttatagagg atattgataa atctaaaaag aatacatctc aagttcaaat
     2461 actaattgat aatttgttag gtaaagcaaa aaatgattac tgcaaagaaa atggttttgg
     2521 aatatataac cctgaaaaca aagttcaaat tccagaacaa tttgatttta aagatgattc
     2581 taatagctca tcaaagctta ataaaattat ctcaaatata agtttatcta ctcgtgagga
     2641 ttctgtttct gtagcaacaa aaaaagaagc aaaaaaaatt ctcgatacgg atataaataa
     2701 aataataaaa gtttatgaaa aatttgatga acttattact gaaaatgcag attatgaata
     2761 tgaagttgaa agatggtcag gtaatgtaga caaagtactt ttaggttatt ctacttataa
     2821 cttgactcct attaaatata aagtttctaa tgatactctt gataattatc cattagcaga
     2881 gctttggaga gaaactagta aaaaacttga cttagattac aaattaatac ttgatatctt
     2941 attttatagt ataagtacat gtggatataa tgaaacagaa aaaagttggt tttcatctat
     3001 atattcaaat gtccttgact ctaaaaaaca aaataaaata tatacaaaac ttgaaaaact
     3061 taaatttaat ggacttacaa gaaaactttt aagagtttta ctaaatgagt ttgaccataa
     3121 agaaatattt aactattgtt tagaaatata ctataaactt agaaacttat tgactataga
     3181 acaatgtaaa aaacaaagag ttgaatttac taaaaactat tacagacaaa atgaatactg
     3241 ccctgcttta aatgccccta ttttaaacta ttggcttgat aaagcacatt cgtttattta
     3301 tgatgatatt tcttttacta agtttttcta cactacgttt tcaaattatc agttgctaga
     3361 atataagttt cctttctata ctagcataaa tgtgtttgct cgtgcacatg aattaggatt
     3421 aatgactgaa gagcaagtta atagagaaat gataagtgga tacaacgcat caaatataat
     3481 aagaacattg acaaatccta gatgggctgg cgacacatta gaaaagtacc cttggcttaa
     3541 agataattta aaaactgtta caaaccgaat tatagaaatt gaatcacgta gaggtgatat
     3601 gccaacagaa gtgtctaatc tagctaataa tatacaatac tttgaaggta taaattattt
     3661 tgtaaatatt ttggtcgctc ttggtaaaga aactttccag agaggatatt catatggtag
     3721 caattattca aaaaaagatg ttttaagttc tctacttaaa aattgctatc ctactaaaga
     3781 agatgattca aaaaaattaa aaagtttact taaaaatacc gacattactg agttaagatt
     3841 gatagaatct gctatgtatg cccctcaatg ggtaaacata attgaaaaat atttaaactg
     3901 gaagggtctg aaaagtacaa tttggtactt ccatgcacat ataaatgaag gcttctctgc
     3961 acaaaaagaa tcagaggttg ctctattctc ccctatttca ccacgtagtt tcattgatgg
     4021 tgcatttgat gtagattggt ttttagattc atataaaact attggggaag aaagattcaa
     4081 tatactttac aagtcagcta aatatataac tagtggtggt aactctcata gacgtgcaca
     4141 actctatgct gatgctacac ttggtaagct tgataaagac atgttgatga aagaaattga
     4201 aacaaataga aatcaagata aattgagaag tttctcttta cttcctatga aaaaaggaga
     4261 cctaaaagaa gcaactttcc gttatgaatt tattcaaaag tttgctaaag aaagcaagca
     4321 atttggtgca caacgtcgtg aaagtgaatc aaaagcttgt actatagcgc tagaaaatct
     4381 agctagaagt gctggatttg gagatgtaaa tcgtatgatg tgggctcttg agactgaaaa
     4441 actaaatgaa gttgctcatc tatttaaacc aaaagatatt gatggtactt caatttggat
     4501 agatgttgat gaaaacggaa aagcatctac taaagttcaa aaaggcaaca agtctttaaa
     4561 gagtatacca aaagctattg ctaaacatcc atatgtactt gaacttaaat ctgtccaaaa
     4621 agatttaaag agtcagtatt ctcgtgcaaa acagagttta gaacaagcta tgaaaaatga
     4681 aatagtattt acagttaaag aaattgccaa tatctataaa aatccagtta tctgcccttt
     4741 gattaaaaat ctagtatggg tatgtgatga taatattgga tatgtttata atgataagtc
     4801 taagttcttt atttgcaata tagaaggtga aacttataag ttgacggcaa aaaataaatt
     4861 aaaacttgca catccagttc atttgtttga atctggtaag tggagtgagt tccaacatga
     4921 tttatttagt aagcaaattg ttcaaccatt taagcaagta ttccgtgagt attatagaat
     4981 aaacagtgat gaattatctg aaaaaactat atctcgcaga tatgctggtc accaaattca
     5041 gccaaagaaa actgtagcac ttcttagaaa tcagctttgg actgtcgatt atgaagaggg
     5101 attacagaaa gtattttata aagaaaatat agtggcaaag atgtttgcac ttgcagattg
     5161 gttttcacca gcagatatag agcctcctac tattgaaaca gtagaatttt ttgatagaaa
     5221 aacttataaa agtttagaat tagcttctat acctccaatt ctattctcag aagttatgcg
     5281 tgatatagac cttgttgtaa gtgtcgctca tgctggtggt gttgaccctg aaactagcca
     5341 ttctactgta gagatgcgta tagcaattgc taaagaactt ttattattat taaaaattaa
     5401 caatgtcaat tttgaaggct ctcatgctct tatagcaggt aatttaggtg agtattctgt
     5461 gcatatgggt tctggtattg tacataaaaa agggatgggt gctttgaata tacttcctat
     5521 acattctcaa agtagaggta agatattcct tccattcgct gatgaagacc ctaaaactgc
     5581 tgagattatg tctaagatac tattacttgc tgaagatagt aagattaaag accctagtat
     5641 cttaagtaat ttataagtat aaaaactatc tctaaaatat attggacttt cttaaaaaaa
     5701 ttagaaagtc caatatatac tatactgtaa tttatataga ttaaatcata attacaaata
     5761 atttttatat aaacttctct tatacattat ctttacggta cttattatat cacacaaaaa
     5821 gtatttttct tctatcttca ctagaatctt ttaaccctat aaaatctata taaaaaatct
     5881 ttttctagag atttgtttaa tatggcattt tcttgaaaat ttataaacct tatctctaat
     5941 gaagatttat ttataggtat aaccttatgt ataaatctta gatttactat gtattgtcta
     6001 tgggtcataa agaaattagg aggtaggcgt ttttttaaat catcaaagtt ctcataaaac
     6061 tttattatat cttcatcttt agtgtgtaga ttaattgctc ttgaattttt ctcaaacata
     6121 actatatcat caaaattaat catatgtatc tgcttcctta ttttatatac aaaaagggtg
     6181 tcttctataa aattattatt agcaatctta tgggagttaa tatggtctat tgctatgtta
     6241 attgaatcaa gtattttttc ttttttaaat ggtttaacta caaaatctat aggatgcact
     6301 ttaaagcttt caagactata atcagcgaaa cccgtcacaa atactatatg tatatcctta
     6361 tctatagaaa gaatttcttc agcagaatca atcccatttt tatatggcat atcaatgtca
     6421 ataatcaata aatctatttt ctcatttttt ataaattcaa ttgcctctat accatcctta
     6481 acttttttaa cataatctac atatccagac tctttagcta tatttgctat atcatctctt
     6541 acaatcttat catcatcaca aatcaaaact tttatcatag acttttctcc tcctaactaa
     6601 taatattttg ggattaagat tacaaattca gttaaaaaat catcactatt tacaagtata
     6661 ctataatcat acttatctaa tatattttta actaaatgta gcccatatcc cctatcttgt
     6721 ccttctttag tactaaatcc attccaaaat atactttcaa cattatgtat tttaggacca
     6781 ttatttttta tagctataat atactgaaat tcatcttcat atgcatgtaa tcttatatac
     6841 ttcttttctt tttttatagt agacaaagac tctattgcat tatctattat gtttaatagt
     6901 actgaactca tatcattagg ttcaatctct aaattatcca taaaatcttg aatgtagaca
     6961 tcaaaagata tatccttttc aagacattcc tcgtatttta atgaagctat agcatctaaa
     7021 taagatactt ttccatacct tgaatttttt gatttaaagc tatttgatat attcattata
     7081 tattctttgg catcttcatc tagcccagta tttaatagag aatacactac ttgcaaatgt
     7141 ttcaaatagt catgtttttg acttcttaat ttgataatta tattatcctt atgatttatt
     7201 ttactatcta gtttttgtaa tttatattcc tgttcattat tattatttat caatcttata
     7261 actttaaagc ctgtataaaa acttaaaata attaaaacca aaaaaagtat aatatttaat
     7321 actccttcaa gtaaaaaact atatcctaac cttaattgta tatatttagt gccctccata
     7381 aataaaatca atgcaaaaca cgatataaaa aatgttaata tgcctctttt taatatgtct
     7441 aattctttgt aaaatttata cttattcatt ataagattgc taaatataaa caaagttagt
     7501 attactatag gaaatacaaa attgttatcc atgattcccc tcccctctac aatttatata
     7561 gggattatat cattttaaaa aaattatgta aaaagttaaa atttacatat atattaatta
     7621 aacttgtaaa aatacagatg aatttgtaca attaactact taaaatttga aaatacttac
     7681 tttgctatat attttagata aatatataat tgatttagtt aagaaaggaa gtggtaaata
     7741 tgaaagataa tttgaaaata gtactaaggt atataaaaag ttataaagct aggtctttag
     7801 caattgttgt gagtataatt cttgctacat cactcgttgt tggtattggt actctctcaa
     7861 gaagtgctca gcaagcagat gtagatagat tgaaaagaga gcttggaact gaccatgtat
     7921 actttaaaaa tataaagaaa aatcaattaa agtccataaa atctagcaaa gatattaaaa
     7981 atctgggtat aacatcttac tatgcgtcta cagatgttaa tgaaaaatta cctataaaca
     8041 tactatatac aaataaaaat tatttgacta gtcattctaa acttataaaa ggtcgatttc
     8101 caaaagctag caatgaggtg gtagtcgaga aatggatttt aaatagtatg ggcttaaaac
     8161 cagagataaa tcaaaacctt acttttaaat tatatcaaaa agaaaatcca gaaactttta
     8221 aagttgttgg aatccttgaa gatagattta tagagaaaaa aaagggacaa tgtgaaattt
     8281 tttctaatct caatgaatca gaactgaatg attttacaac ttatattgaa tttaatgaag
     8341 gcagtgatat aaatacaaac atagacaata taataaaaaa tgctatgtta gatgagaaaa
     8401 gtataggaag aaactcaatg cttatagaat ctactatgaa aaacggtact ttagataatt
     8461 caagtaaata cgttgcaatt gctatgagtc tatttgctgg tattgtaata tatagtatct
     8521 atgttatatc aatataccaa agagtacagg aatatggcgt cttaagagct attggttcca
     8581 ccaactttag gattttcaaa cttatgcttt atgaactttt catacttgct ctaatagcaa
     8641 tgcctatagg tatatgtata ggtatgggag gtgcacaaat atttaatagg tctgtaggaa
     8701 atattcaatt tgaaggaaat ataaatgtta caccttttgt tattccagat aaaataattc
     8761 tcttatcaat tgcatgtaca atactaataa tactaattat aagtttcttt acatacttaa
     8821 aaataagaag aatttctcct attgatgcta tcagaaaaac ttttggagct gataaaaatg
     8881 taaccaaagt taattattta atatcaaaat taactttaaa tatgtcagta acaaaatcta
     8941 tatctgcgaa aaacatcttt agaaataaaa aggggttcat tataattgta ttatctatga
     9001 gtataggagg tataatcgtc ataaaagaca attataaata ttctttttta gatgtgcaaa
     9061 acaaatctac tcaagaacaa acatatatga atgcagattt tattctatca aatgtttatc
     9121 tcaatgaaat tggtaacaat acttctttta aagatataaa agggtttaat gataatcaga
     9181 tagataaaat aaaaaatata gatggaattg acaaagtcaa aactgctagt atcttaaaca
     9241 caagaataga ggtaaataaa ataaatagat tagattacta cgaagctata aattcaactc
     9301 cttattataa gctaaatcct ctgttcataa aaaataagac tactggcaaa tatacactaa
     9361 agcaaaagct aaaaggttat aatgatgaga tgataaagtc attagaaaaa tacttagtaa
     9421 gtggaaatat aaacttgaaa aaaatgaaaa aagaaaattt agctattgta tatgtaccag
     9481 aaatttctaa aacacagaaa tacacaaaga cttttactcc tggtgaagga acaccagctg
     9541 tagatataaa agtaggtgat actataaaag tcaaatatcc aaagggtggg atggatacag
     9601 gtctttatga aagtctaaaa gataattatg aatacttaga atatgaattt aaagttggag
     9661 ctattataag ttatcctttt gcagataatg gtatgtactc tggagatgat ggtgtagatg
     9721 taataacgag tagcgaatac ctaaaaaaat taactggtgt agataagtat aacttagttt
     9781 atgcagacct taatgaaaat gcaaacaaga aaaaaataaa taacctttta ggtaaaattg
     9841 gaagtgaagt tcctggaact acaacagtag atatgatggt agaaaaagaa aattctgata
     9901 agatgacatc tagaactatg atttatagtt atggtatagt agcaataatg ttcataatca
     9961 gtgtacttaa cataataaac aacataagtt ataacttaac ttcaagaact agtgaatttg
    10021 gaatgttaag agctattggt atctctgaga gaggctttaa aaatatgata ctttatgaag
    10081 gaatattata tggtgtactc tcaagtataa ttactatcgt gtcaggaatt ataatacaat
    10141 ttaaaatgta ttatatgcag ggctttgtat catatggatt gggattttca atagattaca
    10201 aaatatatat actagttgtt gtagcaaata taattgtagg tattctagct acatatattc
    10261 catcaagaaa gataaataaa attagtatag ttgaagctat aaacattaca gaataggagg
    10321 aataattatg cctatattgg aaactattaa cttaggtaag atttatggaa aaaaggaaac
    10381 atctgtacat gcactaaaga atgcaaattt aaaaataaat aaaggcgaat ttgttgctat
    10441 aattggacca agtggaagtg ggaaaagtac atttctacat ttagtaggtg gtcttgaaag
    10501 accaagtaat ggtactataa aggtagatgg taaagatata tgttgtcttt cagataaaga
    10561 attagcaaga tatagaagac aaaaggtagg atttgtcttt caacagtata acttaattcc
    10621 tgtattaaat gtaaaggaaa atatagaatt accactaaag ttagacaata aaaaaataga
    10681 taaagaatat attgatgacc ttataaattt attgggtctt aatgaaagga aaaatcatct
    10741 tcccaaccaa ttatctggtg gtcaacagca aagagttgct attgcacgtg ctttatctgc
    10801 aaaaccaagt ataatcctag cagatgaacc aacaggaaat ttggacagta aaactacaga
    10861 agaagtcatg gatttactaa aaagttctat aaaaaaatat aaccaaacac ttataataat
    10921 aacacataat gaaaatattg ctagaaaagc agatagaatt atatctataa tagatggaga
    10981 gcttaaatta acactctaaa actatttata gaaaagagga tttaaatgtt attgatttta
    11041 ggtaatttaa atttaaactt ggattttgac tacagaataa taagagaaga aaatgaagat
    11101 gtagatatat ttatagatat taattatcgt agcttagata tagatactga tggttctaac
    11161 ttatttaatt ctagaataca atttccattt gtaagagcat tgattctaag gctaaataag
    11221 aacaatcaat gtatgacaat tcatttgtta agagatatag atttattctc tgcttttgcc
    11281 aactttgaag tggattatac tgactctata ataaatataa aaaatcaaaa tgagaaagta
    11341 attttaaata aatctatcaa aaaatagtaa gggggcctta ataggcc
//
LOCUS       DAYAPA010000131        11269 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.134, whole genome shotgun sequence.
ACCESSION   DAYAPA010000131 DAYAPA010000000
VERSION     DAYAPA010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11269
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.134"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            <1..1316
                     /locus_tag="ACGI59_003200"
     CDS             <1..1316
                     /locus_tag="ACGI59_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFG9740975.1"
                     /translation="TSINFILILINIFIAFFLYKLTSYFIDFRNHWIFKIILILGYSI
                     ISQMIIWLSDPINILFTLFGYFILLIICSKSKLIPIISVVMILYPIIIGLNFLFINNP
                     IYEELIHSKEYILILSIVQIISALIKLLFWFLIHIFLKNRLVNIKQYLSNKIWIFIDM
                     VCISSFLSILIAIILPPTNLIPMGSNKNIILGNLPYGTYLIILSAILANIGVILLLQP
                     LIENVKMKIEKQANHLKEKYYNLLENQQIQIRKMRHDMNNHFQMIESYLEIEDIAGAK
                     KYFNQLKIGIGTLSGKQFCKNQALNSILNLRHTSLEENGVDVHFNIDIDNHLGIEPID
                     LCTIFSNSLDNAIEASLKIPDLSNRKVVLKARCEKEYFSYLLTNNKVNPINRKQELFI
                     SDKKDSNSHGYGIENIREIVNKYNGKLDISYTESEFSLFLYIRLF"
     gene            complement(1652..2221)
                     /locus_tag="ACGI59_003201"
     CDS             complement(1652..2221)
                     /locus_tag="ACGI59_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HFG9740976.1"
                     /translation="MKKKLEDMTLEELWNLFPIFLTTHKECWKNWFKEEQYRLTGILP
                     MQKIVRISHIGSTSVSTIYAKPIVDILIEIIPDYNMKDIKDLIESNGYICMSESSSRL
                     SLNKGYTVEGFEERVFHLHIRYVGDNDELFFRDYLIDHPDVAKEYEKLKLDLWKKYEH
                     NRDAYTDSKLDFVREFTEKAKVLYSNRYN"
     gene            2624..3196
                     /locus_tag="ACGI59_003202"
     CDS             2624..3196
                     /locus_tag="ACGI59_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea carboxylase-associated family protein"
                     /protein_id="HFG9740977.1"
                     /translation="MEYIINPCSGIKIKITRGQKIEIVDIEGGQVVDFFAEMKDDSTE
                     FISPGVTIDCNESLRLQVGNLIYSNKYRPMFKILYDDVREHDLLHPCCRPEMYDFFYH
                     NGENHPNCFDNINRTLGTSHQIIHPINLFMYTKVNTDGSIEVCEPLSKANDKIILEAL
                     ENMTLGIAACSVSESSCNSRKCTSIKILVD"
     gene            complement(3875..4777)
                     /locus_tag="ACGI59_003203"
     CDS             complement(3875..4777)
                     /locus_tag="ACGI59_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HFG9740978.1"
                     /translation="MLPQDYYGLIVILGVILGVMGAFYNWFTLYVQSLYKKIPKIGTF
                     GKLLIPFIMAGILGLVMPDVLGSGHNLVEELTSHKYIFGMVVLIFVIRFIFSAVSFGS
                     GAPGGIFFPLLVLGAFIGGIFGMVGVKVFGMNPDYVNNFVLLAMAGYFTAIVRAPLTG
                     IILIFEMTGSVSQMLSLSVISIVAYIVATLMKSKPIYESLLERLLEKNGEPVHKERGE
                     KILDQFSVMFGSPVANKAIQDVQWPENCLLVAIQRGGEEIIPRGKTILRPSDVIVTMT
                     DERDSGAVYDKMENLCKEHTAHIF"
     gene            complement(4904..5023)
                     /locus_tag="ACGI59_003204"
                     /pseudo
     CDS             complement(4904..5023)
                     /locus_tag="ACGI59_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
     gene            complement(5566..6159)
                     /locus_tag="ACGI59_003205"
     CDS             complement(5566..6159)
                     /locus_tag="ACGI59_003205"
                     /inference="COORDINATES: protein motif:HMM:NF012861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HFG9740979.1"
                     /translation="MVGAVAGLIVLLYRILLGQAGTWLSQILKFVEGSTIKIIAWFAV
                     LAVLAWIVSKLVDWEPMISGSGIPQLEGEMTGKIKQVWWRVLPTKFIGGFLSLMGGLA
                     LGREGPSIQLGAMAGKGISRALDRGKTEEKFLLTCGASAGLSAAFHAPLAGVMFSLEE
                     VHKNFSVSVLISVMTASLTADYISANFLGMESVFQFD"
     gene            complement(6391..6927)
                     /locus_tag="ACGI59_003206"
     CDS             complement(6391..6927)
                     /locus_tag="ACGI59_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HFG9740980.1"
                     /translation="MIGLKCGMVKLLEHQMIWDKSAKDVIILLKSIWDKTAIDIQHIG
                     STSIPSISAKPIIDIVVGVASLEEAKLYLERLEQCGIVFRGQDVPKQLLFAMGDFEKN
                     TRTHHIHVVEWNSVAWNNYINFRDYLNTFTDKAKLYDSHKQKLALQFEDNRKIYTQRK
                     QELINILLEEARLWRINT"
     gene            complement(7000..7413)
                     /locus_tag="ACGI59_003207"
     CDS             complement(7000..7413)
                     /locus_tag="ACGI59_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003507530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HFG9740981.1"
                     /translation="MKMPKESIDTIMFAPCGMNCMVCYKHCYHKKPCAGCLNGDTGKP
                     EHCRKCKIKDCVCQKGLFYCFECFNFPCKLIKNLEKSYNKRYQASLMENSKFVQQYGL
                     EKFMQKQKERYTCSKCGGIISIHDRECSECQEKVK"
     gene            7879..8433
                     /locus_tag="ACGI59_003208"
     CDS             7879..8433
                     /locus_tag="ACGI59_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HFG9740982.1"
                     /translation="MDEKILELLQEMQGNISNMQGSISGIEGTISDMQSNINGIEDTI
                     SGMQGTISDMQGSISSMNGTISDMQGSISSMDGTINSMQDSITDMQVEIKEVNSRLDN
                     MEPDIKDLKTGQVETSSRLDTIQSDVTEIKSDVKINRNTLDTVIKHTSSLVKDTSDIK
                     NDLNNIEIVTSKNSNDIAKLKSVK"
     gene            complement(9041..9244)
                     /locus_tag="ACGI59_003209"
     CDS             complement(9041..9244)
                     /locus_tag="ACGI59_003209"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFG9740983.1"
                     /translation="MCLDAVIYMLQTGKQGADKKYLDNMFFVKGYVEELLFLDNSYDI
                     VFSCIVFHHCTNVQNIFCIFWKW"
     gene            complement(9446..9979)
                     /locus_tag="ACGI59_003210"
     CDS             complement(9446..9979)
                     /locus_tag="ACGI59_003210"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFG9740984.1"
                     /translation="MQYISYYHSPIGNILLASDNIGLTGLWFENQKYYAYNLDQEHQE
                     KELPIFEQTKKWLDIYFSGKEPNFTPSLHMVGTPFQTTVWKILQQIPYGKTITYGEIA
                     YKVAQQKGISKMSAQAIGGAVGHNGISIIIPCHRVVGTNGSLTGYAGGIDRKIKLLSL
                     EKVSVQHYFIPKKGSAL"
     gene            10746..10934
                     /locus_tag="ACGI59_003211"
     CDS             10746..10934
                     /locus_tag="ACGI59_003211"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFG9740985.1"
                     /translation="MPYKYFTEFCLLKATQFLKGTNELISDIAIKVVFHQIIYFSKCF
                     KEKTGYSPKVYRNIKKEI"
BASE COUNT         4025 a         1897 c         1356 g         3991 t
ORIGIN      
        1 ctacaagcat aaattttatt ctaatattaa tcaatatttt catagccttt tttctttata
       61 agttgacttc ctattttata gactttagga atcattggat tttcaaaatc atattgattt
      121 tgggctactc tattattagt caaatgataa tatggctttc cgaccccata aatattttgt
      181 ttacactatt cggatatttt atactactta taatatgtag caaaagcaaa ttaattccta
      241 taatatcagt tgtcatgata ctttatccta ttataattgg gcttaatttt ctttttataa
      301 acaaccctat atatgaggaa cttatacact caaaagagta tattttaata ctttcaatag
      361 tccagataat ttctgctcta attaaattat tattttggtt tttaatacat attttcctaa
      421 agaatagatt ggtcaatata aaacaatact tatcaaataa aatatggatt tttattgata
      481 tggtatgtat atcttcattt ttatcaatat taattgctat tattctgcca cctactaatt
      541 taatccctat gggtagtaat aagaatatta tcttaggcaa cttaccttat ggcacttatc
      601 taatcatctt gtcagcaatt ctggcaaata tcggtgtgat acttctttta cagccattga
      661 ttgaaaatgt aaagatgaag atagaaaaac aagcaaatca cctaaaagaa aaatactata
      721 acttactaga aaatcaacaa attcaaatac gcaaaatgcg acatgatatg aataatcact
      781 ttcaaatgat tgaatcgtat ttagaaattg aagatattgc tggagctaaa aagtatttta
      841 atcagttaaa aataggtata ggtactctta gtggaaaaca attttgcaaa aaccaagctc
      901 ttaactcaat tttaaatctt cgtcatacaa gtttagaaga aaatggtgtt gatgtgcact
      961 ttaatataga tatagacaat cacttgggta tagaacctat agatttatgt actatttttt
     1021 caaattcttt agataacgct atagaagcat ctttgaaaat tcctgatttg tcaaatagaa
     1081 aagtggtatt aaaggcacgt tgtgagaagg agtattttag ctatttattg acaaacaaca
     1141 aggtaaatcc tatcaataga aaacaagaac tatttatttc agataaaaaa gatagtaatt
     1201 ctcacggata tggtattgag aatattagag aaattgttaa taagtacaat ggaaaattgg
     1261 atatttctta tactgagagt gaattttctt tatttcttta tattagatta ttttagtcta
     1321 aaattagatt atcttatata atacaaatta gattgtttga tactatgtaa aattataata
     1381 acttataatt aaatatttac acatatattc attaacttaa aatttaatca aatgtgatat
     1441 tttatttacc ttagtaaaaa tgtgtgtgtc tcaaaataaa ctttttagtt gatgaggcac
     1501 tcttttttac atgaaatcaa atatattttt atcctttcaa caatgaaaaa tggatttatc
     1561 tctccatttt gaaacaagcc ctttttctat taattacttt cttaaaaaat ataatacgat
     1621 atataagata aatatctaat tgtacctatt tctaattata tctattagaa tacaacacct
     1681 ttgccttttc agtaaactcc ctaacaaaat ctagtttact atctgtataa gcatcacgat
     1741 tatgctcata cttcttccaa aggtccaact taagtttttc atactccttt gcaacatctg
     1801 ggtggtcaat taagtaatct ctaaaaaaca actcatcatt atcacctaca taccttatat
     1861 gcaaatgaaa aactcgttct tcaaacccct ctactgtata ccctttatta agtgaaagcc
     1921 tactactgct ctcagacata caaatatatc cattcgactc aatcaaatct ttaatatctt
     1981 tcatattata atcaggaatt atctcaatca gtatgtcaac aatgggcttg gcataaatcg
     2041 tactcacaga agtacttcca atatgactta ttctaacaat tttttgcatt ggcaatattc
     2101 cagttagtct atattgttct tctttaaacc aatttttcca acattctttg tgagtagtca
     2161 aaaatatagg aaacaagttc cataactcct ctaaagtcat atcttccaac ttctttttca
     2221 ttagttacct ccttttaaat ttgtacttta tattttttct ttttcaaatt tacttagtta
     2281 atatttaact ttatttttta tcaaatcatt tttctaatta tgtaaatttt tctaaattta
     2341 gtttttaatg tattataaca gatttttacc cttcttacac ttttgtatct atttaaatat
     2401 tagaacattt tttcttactt ctaaatacag taagattgct taaataccaa ataggagcaa
     2461 aaaaactctg taatctaaaa aaaactacaa catctttatg agaattaaaa aatgatagac
     2521 tatgttatca acattaagaa acagatagaa aagaaacaga gtatgtgcta aaatgggaac
     2581 atagaacaga atgacaaatt ggaatttatg gaggacatta aatatggagt atataatcaa
     2641 tccttgtagt ggtataaaaa ttaaaataac tagagggcag aaaattgaaa ttgtggatat
     2701 tgaaggagga caggtcgttg atttttttgc agaaatgaaa gatgattcaa ctgaatttat
     2761 atctccaggt gttacaattg attgtaatga gtcattaaga ttacaagttg ggaatttaat
     2821 atattctaat aagtatagac ctatgtttaa aattttatat gatgatgtaa gggaacatga
     2881 cttgctacat ccatgttgta gacctgagat gtacgatttc ttttatcaca atggagaaaa
     2941 tcatccaaac tgttttgata atattaatcg cacacttgga acttcccatc aaattattca
     3001 ccccattaat ttgtttatgt acacaaaagt caatacagat gggagtattg aagtatgtga
     3061 acctttatca aaagccaatg acaaaattat tcttgaagca ttggaaaaca tgactttagg
     3121 gattgctgca tgcagtgttt cagaaagttc ttgtaatagc agaaaatgta cttctattaa
     3181 gattttggtt gattgacaaa tttcatttat cgaaaaaata tcaaaattaa aaaatgacgt
     3241 caaaggcgca aaagggtaac cacttgcgct ctcaattcat tatggtttgt tacgtattta
     3301 tgggtaaaaa tcccttgatt ctatgatgtt tttcagacac aattacaaat gagtaaggat
     3361 tttatactca tactctaaaa aatagcattt tattgcgtaa cagaacgaaa aaacagtgtg
     3421 tacctttttc ttgcaataca cactgtattc atagccactc tagctgttaa ctgttaatta
     3481 tctcataata cccttactgg tattcatcta atcttaaccc cttttaatta ttttcattta
     3541 taaataaatt ctattcttcc caaaatatcg taattaaaat atattaatta aaatatccca
     3601 ataagaatat ctgaaaaatc ttctacctat tttttcaaac cttttattcc cattcctcat
     3661 acaatagtgc aacttttagc acagtataaa tataccatta gtccatataa tctcattaac
     3721 aaatatatac aattattttc ttcaaactct tttatatcta agatatcaca ttttcatttt
     3781 cttatattaa ttccttaact ctctttcaaa ttaccaattt tttaatcaaa tcttcattaa
     3841 atattctatc attattaaac attcatcatt atccctaaaa aatatgtgcc gtatgttctt
     3901 tacacaaatt ttccatctta tcatacacag caccactatc tctctcatct gtcatagtca
     3961 caattacatc acttggtcta agaattgtct tacctctagg aataatttct tctccacctc
     4021 tttgaattgc taccaaaaga caattttcag gccactgtac atcctgaatt gccttgttcg
     4081 caacaggaga gccaaacata acagagaact ggtctagtat cttttcgccc ctctctttat
     4141 gtacaggttc tccattcttt tcaagtaacc tctcaagaag actttcataa ataggtttag
     4201 acttcataag tgtagccaca atatatgcca caattgaaat tactgatagt gaaagcattt
     4261 gactaacaga acctgtcatt tcaaaaatta atatgatacc agtcaaaggg gcacgcacaa
     4321 tagctgtaaa atatcctgcc atagccaaaa gcacaaagtt atttacataa tcgggattca
     4381 ttccaaaaac tttaacacct accataccaa atattccacc tataaatgct cctaaaacaa
     4441 gcaatggaaa aaatattccc cctggtgcac ctgacccaaa actaacagcc gaaaatataa
     4501 aacgtataac aaaaatcaaa acaaccattc caaaaatata tttatgactt gtaagttctt
     4561 caactaaatt gtgtccactc cccagaacat caggcataac aagcccaaga ataccagcca
     4621 ttataaatgg aatcaacagt tttccaaacg taccaatttt aggaatcttc ttgtataaag
     4681 actgcacata tagagtaaac cagttgtaaa atgcccccat aactccaaga ataacaccta
     4741 aaatcactat taatccataa taatcttgtg gcaacacatt tccaatctga acctcccatg
     4801 actaaagtca cagggttcct gcttcataga attttgcata ctaaaatatg tcttactaat
     4861 tctccacagg ctatccccgt agttcctacg gttcttacat aagttattta ttttgaattg
     4921 ttattagtct taatccttct tttaatatat ttatacttgc atttatatct ctatcgtgag
     4981 tttcattaca actaggacaa atccattctc ttataataat gttaacaaat gtaattattt
     5041 aaaacaatta tttagaaggt tttatattta attttaaata acatatttaa gaaccgtaag
     5101 tcttaacttt tatcattttt gaggttgtcg ttcacataag ttcgctacta cctatgcagt
     5161 tctcttatga acttcttaca ctttcatgca agcacagact atatcttatc cttcggcatt
     5221 atccgttaag gtctacccac ttccactacc aatagcttgt agtgtacttc cctcaagagg
     5281 aatagtcgtt gaagtttctc ctattcggag tttacctgct gattgcccat tttaaagtac
     5341 ttagggttta accttatacc atctaaccaa ttttttctac tttcgtaact ttcacactta
     5401 ggtttatttc atccttatgt tttagtttgg ttagctttag gggtttccag caattcaagt
     5461 agtattggat aggttctatc ctatctctac atataggttt ccctatatgc tgactatttt
     5521 agctactaac tcatgactaa agtcacaagt gttcgtagct attttttaat caaactggaa
     5581 tacagactcc attcctagga aattggctga aatatagtct gctgtcaaag atgctgtcat
     5641 cacagaaatt aaaacagata ctgaaaagtt tttatggact tcttcaagtg aaaacataac
     5701 acccgctagt ggtgcatgaa aggctgctga taatccagca cttgccccac aggttaacag
     5761 gaatttttcc tctgtcttcc ctcggtcaag agctctagaa atacctttac ctgccatagc
     5821 accaagctga atggaaggac cttcccttcc caaagcaaga ccacccatga gtgataagaa
     5881 tcctccaata aattttgttg gcaacactcg ccaccatact tgttttattt tccctgtcat
     5941 ttccccctca agctgaggga taccacttcc agaaatcatt ggctcccagt ccaccaactt
     6001 tgatacaatc cacgcaagta ctgcaagtac tgcaaaccaa gctattattt ttattgtaga
     6061 accttctaca aattttaaaa tttggctaag ccaagtacca gcctgcccta aaagtatcct
     6121 gtataaaagc acaatcaaac cagccacagc cccaaccata agtccttcac caatcaaaat
     6181 tacctgaaag tgctctgccc gtttgataat atgagatgta tcctttttca tactattcct
     6241 ccttcatcca ataattctac acaatctgaa tctccaatga ctaaagtcat gagtgtgtac
     6301 agatattttt taatcaacaa aaatagattt ttacttacta aatataagaa aataattgct
     6361 caatttctca ataaatgtga atttattctt ttaggtattt attctccaca gtcgtgcttc
     6421 ttccagcaaa atatttatta attcttgctt tctctgtgta tagattttac gattgtcctc
     6481 aaactgtaat gctaattttt gtttatgact atcatagagt tttgctttgt ctgtaaaagt
     6541 atttagataa tctcgaaaat tgatataatt attccatgca acactattcc attcaacaac
     6601 atgaatatgg tgagtacgag tatttttctc aaaatctccc atagcaaaaa gtaattgttt
     6661 tggtacatcc tgacctcgaa aaacgattcc acattgctct aacctttcga ggtataattt
     6721 agcttcctct aaagaagcaa cacctacaac aatatcaata ataggctttg cgctaatact
     6781 gggaatagaa gtgcttccaa tgtgttgaat atcaatagca gtcttatccc atatactttt
     6841 taataatata atgacatctt ttgcactttt atcccatatc atctgatgtt ccaataattt
     6901 aaccatacca cattttaaac ctatcactat ataccacctc aatataatcc acctttatat
     6961 atattacaat tttttctatc ttgataaata gtgatttagc tatttcactt tttcttgaca
     7021 ctcgctacat tccctatcat gaatagatat aattcctcca catttagaac aagtatatct
     7081 ctccttctgc ttttgcataa acttctctaa accgtactgc tgaacaaatt tactattttc
     7141 cataaggctt gcttgatatc tcttgttata acttttttca agatttttta tgagtttaca
     7201 aggaaaattg aaacattcaa aacaataaaa caaccctttt tgacagacac aatcctttat
     7261 tttgcactta cggcagtgtt ctggctttcc tgtatcgccg ttcaaacaac cagcacatgg
     7321 ctttttgtga taacaatgct tatagcaaac catacaattc attccacaag gagcaaacat
     7381 aatcgtatca atactctctt ttggcatttt cataacattt tactcctttc aaaaatccaa
     7441 tttgtcgaat tgttatatat tcatattgta acataaaaaa tagctaaaaa attcatgcac
     7501 tttcattgcc aataaatttt caaggaaata tgaaagttta aaactaagaa taaaaagaga
     7561 taaaaaggaa gctactaatc aaaatattca aacacaagta atcaatataa ataacttaag
     7621 gtatatttaa aagtatatga tattaccagc agttttaaat acaaatataa ccacacatgc
     7681 ctcaaaaaca cttattacag tatcttagaa acttagacaa tacataagat tcattaataa
     7741 aaaagttctc aatatagata tatttaacca tgttttgaat agtagataat catatgatta
     7801 aaaattaaat atgacatagc gtttgtggaa tataacacct aaattatgtt ataataataa
     7861 agcaggagtg tgatagtgat ggatgaaaaa atattagagt tattacagga aatgcaaggt
     7921 aatattagta atatgcaagg tagtatcagt ggtatcgaag gcactattag tgatatgcaa
     7981 agcaatatta atggtatcga agacactatt agtggcatgc aaggcactat tagcgatatg
     8041 caaggcagta tcagtagtat gaatggcact attagcgata tgcaaggcag tatcagtagt
     8101 atggatggca ctattaatag tatgcaagac agtattactg atatgcaagt tgaaattaaa
     8161 gaagtcaata gtaggttaga caatatggaa cctgatatta aagacttaaa aactggacaa
     8221 gttgaaacta gtagtagact tgatactatt cagagtgatg ttacagaaat taagtcagat
     8281 gtaaaaatta acagaaatac attagataca gtgattaaac atacctcttc attagtaaaa
     8341 gatacatcag atattaaaaa tgatttaaat aatatagaga tagtcacatc taagaattcg
     8401 aatgatattg ctaaattaaa atcagttaaa taatatacta tagtaaaaaa tacctgaaaa
     8461 tttcacatac ttctttaaag ttataccaaa ttttatacag aaatactgat tatccgacaa
     8521 cacactttac ttataaataa tttattaaaa aaatactcat tttatcctaa aattaacacg
     8581 actaaatgag tcttgatata ataataaaaa ataagggaga ttgaacacta aaaagttgtg
     8641 tatctccctt atattctata atatagttat ataatttttc agcctaaaat aaaagtccat
     8701 ctttgattaa aacacatccc cttatcactt ataaaaggat aaaatcttgt tttcattttt
     8761 ccatttatat cttcttgctt aagagtaata attttttctt ttgatacttt aagtgattgg
     8821 ttaaatttaa ccaaacgact aagttttgtt caacatctat ttttcatatt ttccaatact
     8881 taatctattt tttataaata attcttttat ttctaactca cttacatttt ttacataaaa
     8941 cgaagcatat aaatttcaac cttatcttgt atggttctta atacttaatg agtagcttaa
     9001 gtatcaatca ttataaattt ttcccttaca tttaacactc ttaccatttc caaaatatac
     9061 aaaatatatt ttgaacattt gtacaatgat gaaatacaat acatgaaaat acaatatcat
     9121 aactgttgtc taaaaataga agttcttcaa cataaccttt cacaaaaaac atattatcta
     9181 agtatttttt gtctgctcct tgttttcctg tttgtagcat ataaattact gcatctaaac
     9241 atataactac tcacacgaaa aaagaaaata aacttccaaa atacaagctc taaaaactac
     9301 ctctaaagtt gtataatgtt tactaggaat aaggttgtat aacatatagt ttaaataatc
     9361 attttttaaa attgcaagtt attgctttca aaattagatg tttttatatc aaataaatga
     9421 ttaagaattt ctaaatttaa tggctttata atgcactccc tttctttggg ataaaataat
     9481 gttgcacaga tactttttct aaagataaca attttatttt tctatcaatt ccaccagcat
     9541 acccagtcaa actcccattt gttcctacaa cacgatgaca tggaataata atagagattc
     9601 cattatgccc tactgcacca ccgattgctt gtgctgacat tttagatatt cctttttgtt
     9661 gagcaacttt atatgcaatt tctccataag tgattgtttt accatatggt atttgttgta
     9721 aaattttcca cactgtagtc tgaaaaggag tacctaccat atggagtgat ggtgtaaaat
     9781 tgggttcttt tcccgaaaaa taaatatcta accatttctt tgtctgctcg aaaattggca
     9841 attctttttc ttgatgttct tggtcaagat tataagcata atacttttga ttctcaaacc
     9901 aaagaccagt taacccaata ttatctgatg ctaaaagaat atttccaatt ggactatggt
     9961 aatagctaat atattgcata caattcctcc ttatattctg aaaacttcac tcaaatcatt
    10021 agacacttct tttctatcta ccattatgta aaaacatact aatataattt ccttctaact
    10081 acactttcgt tataatatct atcctcatat catattagtt ttatatttac ctgcatctgt
    10141 tcatcctttt ttccctttcc ctttgatgaa tataaaattc atacatttaa gcttcctact
    10201 gtattaactt ttatagaagt gtatattata attttatcac taaataggcc aaaaaaatat
    10261 cgatatagca tcaagtaata tcataatact ttcaaatggg aaaagtaaga ataggctgta
    10321 aaaaagaaaa gtatttttat gttagaaatc cagtaaaatc attagtagat agaataacag
    10381 aaaacgaaca atttgctatt tatcatttta cacataaata taattgacgc aataaagtgt
    10441 tttctattct acaacgatta ataaggttag aaaaaagtaa aaatgaactg tatatgtatg
    10501 aagcattagt ttctgtttct ttcctttggc ttattatgat aaaaaatatt gttttgctac
    10561 caataaaaca caaaaatata attaatatcc atatgcaaaa aattttacgt tatattgaaa
    10621 aatactattg ttttacatta tattgaaggg tactattgtg aaaacttaat tttggagtac
    10681 tatcaagcag tggtaatatc aataaatcag agtgctccag atgatttgaa ctaagtttaa
    10741 atactatacc ttataaatat tttactgagt tttgtttatt aaaagcaact caatttctaa
    10801 aaggcacaaa tgagctaata agtgacattg ctataaaagt tgtttttcat caaataatct
    10861 attttagtaa atgttttaaa gaaaaaacgg gatattctcc aaaagtgtac agaaatataa
    10921 aaaaagagat atgatttttt atttcttttg attatccaaa gagtaaataa aaaatattgt
    10981 aaaaaactta ctaacaacgg caatgctttg aactatggta atactttgaa cttttgattg
    11041 gacttttttt gctaaaataa tatatttaaa tagcaaagta atctgtttaa tcaataaaaa
    11101 ataagagata aaagaaatca actaaaactt ttacctctta ttttgatagt ttatcagagc
    11161 taatatttac actcttttta tttttgatac ttcattaaaa atgatgctta ctctccattc
    11221 acttttaatt cttcattaaa aatgatgctt attctccatt cacttttaa
//
LOCUS       DAYAPA010000132        11188 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.135, whole genome shotgun sequence.
ACCESSION   DAYAPA010000132 DAYAPA010000000
VERSION     DAYAPA010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11188
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.135"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            <1..421
                     /locus_tag="ACGI59_003212"
     CDS             <1..421
                     /locus_tag="ACGI59_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF116 domain-containing protein"
                     /protein_id="HFG9740986.1"
                     /translation="IYSNKYNFNPEDIIILIPHCIQENSCKLKVTNDIDNCKECGRCN
                     IGELIKLKEKTNVKIFVATGGTLARKIIMDNKPKAVVAVACERDLTSGIQDIKKIPVL
                     GVFNKRPNGPCVNTNVDIMDIEKAIGFLTGKNILVCN"
     gene            490..1191
                     /locus_tag="ACGI59_003213"
     CDS             490..1191
                     /locus_tag="ACGI59_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HFG9740987.1"
                     /translation="MYPVYGGFWGFDPTMVVLIPAILLTIYAQFKVSSTTNKYLRVNT
                     RRGYTGEQTARRVLDSNGLYDVKIEMVRGHLSDHYDPRRKAVRLSEDVYYGTSITSVA
                     VAAHECGHAIQHAKGYAPLQIRSSLVPVVNFASSISWFLIFLGFIMAGPFLKIGILLF
                     SASVLFQIITLPVEFNASSRAIVQLGNLGIIDESESRQSRRVLSAAALTYVAAALVSI
                     LQLLRLLLIAQRRND"
     gene            1253..2578
                     /gene="rsmB"
                     /locus_tag="ACGI59_003214"
     CDS             1253..2578
                     /gene="rsmB"
                     /locus_tag="ACGI59_003214"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFG9740988.1"
                     /translation="MDAREVGFKVLCDIEKNDNYSNIAINKHFKNLEISDMDRGLATE
                     LIYGVIENKYYLDYIINKLSKIKVKKMSTYVKIFLRMGTYQILFLDSISDYAAVNETV
                     KLSKKYDKKSSGFINAILRNEIRAKETIMDITEEDSVKYLSIKYSYNSWIIKNWIDKF
                     GQEFAEDLLEANNEKPSIYIRTNTLKISREELIEKLSEMGIVCLKVPMVEEAIKVEKM
                     KNIEKNELFKAGLFTIQDISSMIVGKVINPKEGSFVLDVCSAPGGKSTHLATLMNNTG
                     QVVARDIFEHKLKLINSTVNRLGLKNVFVERFDASEIDENSISKFDYVLADVPCSGFG
                     IIRRKPEIKYKKEEELKDITSIQKKILENASKYVKVGGTLVYSTCTIQDVENINIVTS
                     FVEENHNFEFVPIDTVNVDLDNQDKGYLKIYPNIHGIDGFFIAKLKRIR"
     gene            2586..3617
                     /gene="rlmN"
                     /locus_tag="ACGI59_003215"
     CDS             2586..3617
                     /gene="rlmN"
                     /locus_tag="ACGI59_003215"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFG9740989.1"
                     /translation="MEKKKIVLKNFTEDELKEFMKTIDEKPFRGSQIFSWIYKGAKTF
                     DDMNNIPKSLRNKLEEVSCIGHIDIELKLESKVDNTKKYLFLLDDGNIIETVMMDYDS
                     RVTVCVSNQVGCRMGCNFCASTMDGLIRNLEPWEILDQVIKIQEDTGKRVSNLVLMGS
                     GEPLDNFENTKQFLKIINEKNGLNIGYRHITLSTCGIVPKMYELADLEIAINLALSLH
                     SPYDEERRKIMPVANAYSIEEILNACRYYIKKTNRRVTFEYSLIKGVNDSEKEAKALA
                     KLLKGMLCHVNLIPINKVEEREYEKPDKAFIYKFRDSLEKNNIPATVRMSMGSDISGA
                     CGQLRRKYK"
     gene            3637..4389
                     /locus_tag="ACGI59_003216"
     CDS             3637..4389
                     /locus_tag="ACGI59_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HFG9740990.1"
                     /translation="MVYSCASHIGKIRKNNEDYCEGEVIDTEHGPIGIFAIADGMGGH
                     KKGEVASKLAVENIIDFLKENLLQHDNVKIDYIDDILKQAYNNVNSIVHKKSMEDIEF
                     EGMGTTLVTAIVYNNVLYVANVGDSRCYLLTDEKFDKITIDHSVVEELMMAHVITEEE
                     ARRHPQRNRITRAIGTDDMVVVDIFKKEIKKSDIILLATDGLTGFIDDEDIRDLILDY
                     EYERTSNISEELISMANDVSGKDNVSVIVIKV"
     gene            4406..6421
                     /gene="prkC"
                     /locus_tag="ACGI59_003217"
     CDS             4406..6421
                     /gene="prkC"
                     /locus_tag="ACGI59_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861606.1"
                     /note="PrkC, as found in Clostridium difficile, has a
                     kinase domain, two PASTA domains, and a C-terminal
                     Gly-rich repeat region; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing Ser/Thr kinase PrkC"
                     /protein_id="HFG9740991.1"
                     /translation="MGDTILGNRYEIIRKIGDGGMAFVYEAKDRLLNRTVALKVLRPE
                     FVDDDEFLTKFKREAEAVASLSHPNIVNVYDVGEDGKVHYIVMEFVDGKNLKEIIQDE
                     GILDEYTALDITKQIAMALSAAHKKGIIHRDIKPHNILISNEGRVVKVADFGIAKAVS
                     NSTMTNIGSIIGSVHYFSPEQAKGKFVTNNADLYSLGIVLYEMLIGKVPFRGDSPISI
                     ALQHINDDIDFTSEEKVRIPQSVRTTIKKLTEKSSADRYQTAEELIEDIEYIEKNIDL
                     DFIKEYDDFATKKIDEKEINKVVNPTLAKPAPEKVVKPVEVADLDDDEDYYDDFYEED
                     DDEDEEEEEIMRAKKNQRPKSTPSKRTKKKKKKQESPKSRRRLKVIAAVLILILCAQV
                     FLAYKFLFAGGFGNKSLTVPNLVNMTLEEAQSAVEKEGLYLSVKSEEYNSEVDENCII
                     SQTPEGGSTNVKKGDTINVVVSKGSSQASVPNVVGLTLSNAKQLIEENNLKVGTVKYE
                     YSSIYKEGTVLSQSPGAGSSRAQEGDEVNLYVSKGSEKSNTQTPTVPDKKPTTPENNT
                     PTEPGSNSGNSGGNSGGSNSGNSGGNSGNNGSNSGGSNSGNSGGNSGGSNSGDNGGSS
                     GGSNSGDNGGSSGGSNSGDNGDSSGGSNSGDNGGTSSGTGANIGKTE"
     gene            6529..7266
                     /locus_tag="ACGI59_003218"
     CDS             6529..7266
                     /locus_tag="ACGI59_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFG9740992.1"
                     /translation="MKKKTVLITGGARGIGKAMSKAFAKEGYNVLVNFNKSENEAKEL
                     YTILNEKNFSVKLFKADISNREDVEDMVDYCIKEFGGLDVLVNNAGVSQDKLFTDITD
                     EDWDNMMNINLKGSFYCSQVALKYMISEKKGNIINISSIWGISGASCEVHYSITKAGI
                     IGMTKALAKEVGPSNIRVNSIAPGVINTDMLSGYNEEDIDALVEETPLMRLGTPEDIA
                     NCAIFLASDKSNFITGQVISPNGGFVI"
     gene            7364..8248
                     /gene="rsgA"
                     /locus_tag="ACGI59_003219"
     CDS             7364..8248
                     /gene="rsgA"
                     /locus_tag="ACGI59_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HFG9740993.1"
                     /translation="MLEGKIIKGISGFYYVDTYNGIYECKARGIFRKQKITPLVGDRV
                     KISIVDENEKKGILEEIDSRDTELIRPPIANVDKALIVFAIKNPKPNLSLLDRFIVLA
                     EKENLETVIILTKADLDDNDTLETVKNIYELSGYKVIPVSNITKLNIDKVKEELKENV
                     VVFAGPSGVGKSSLLNEIDENFKLQTGVVSDKIKRGKHTTRHAELLKLEFGGMVADTP
                     GFSSLALEDIEEVELKDYFIEFDKFNDCKFGSKCIHENEPNCAIKEAVTNGEISKERY
                     DSYIQLLHEIRQNNSRRY"
     gene            8253..8924
                     /gene="rpe"
                     /locus_tag="ACGI59_003220"
     CDS             8253..8924
                     /gene="rpe"
                     /locus_tag="ACGI59_003220"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFG9740994.1"
                     /translation="MIKLAPSILSADFAKLLEDVRKVESAGCEYLHIDVMDGHFVPNI
                     TLGPLVVKSLKKENINMVFDAHLMIENPDQYIEEFVKAGCDIITVHQEACVHLHRTIQ
                     NIKSHGIKAGVVLNPATPVDTIKHVLPDLDMVLLMSVNPGFGGQSFIPCVLDKIKELK
                     AIIDSQGLNIDIEVDGGISPKNVAEVVQAGANVIVAGSAIFGSDDIQETVNLFRKNAS
                     LEELV"
     gene            8933..9574
                     /locus_tag="ACGI59_003221"
     CDS             8933..9574
                     /locus_tag="ACGI59_003221"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HFG9740995.1"
                     /translation="MKICIVLNGEIEDYEVTRDIIIKECYDCIICADGGANHTYKMEI
                     MPDYILGDLDSVEEEKINFYKNKGVKFEKFPSKKDETDTELCLFLAKTLKANHIDFFG
                     ALGGRIDHTLANIKLLYYLKEDGIYSRILSDKEEMYIVENEEISLYGNPGDTISVIAI
                     NGDAKGVTLTGLEYPLDNYYMKYSVPIGISNVMLSNSCKIKVEQGCVLVVRNL"
     gene            9705..10334
                     /locus_tag="ACGI59_003222"
     CDS             9705..10334
                     /locus_tag="ACGI59_003222"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFG9740996.1"
                     /translation="MVDSIIFDLDGTLWDSTEEVCKVWQDVLSEHKEIELSVTKDLFR
                     SLMGLSFEEIANRLFPNLSEEERMNLLNECSLKECDYLSENGAKLFDDIEDTLKLLSK
                     KYKLFIVSNCQAGYIESFLKAHKLEQYFIDFECPGNTGLHKGENNKLIIERNKLINPI
                     YVGDTQGDANSAKFVGIPFIYAKYGFGNVDVYDYFIDSFKDLLEHDILK"
     gene            10389..11045
                     /locus_tag="ACGI59_003223"
     CDS             10389..11045
                     /locus_tag="ACGI59_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFG9740997.1"
                     /translation="MELQDTIFKRQSVRKFKNQDVSDEDILKMIKAAGAAPSGKNIQN
                     WHFVVIKRRDLMEKIADVITKKQQEILVEMDKVSVDKANRFRKFVKNFTLFYLKAPVL
                     VLVFTKVYNPSGYYELELIDAPKETIDKLFIRNPGMQSLGAAIENFTLSAIELGYGSC
                     WLTSQNYAADEIEAVLEAETGFEKGEYFLGAMLALGVPEDNLKSPSKKPVEEICTFIK
                     "
BASE COUNT         4444 a         1141 c         2182 g         3421 t
ORIGIN      
        1 tatatacagt aataaatata attttaatcc agaggatata ataattttaa tacctcattg
       61 tattcaagag aatagttgta aattaaaagt tactaatgat atagataatt gtaaagaatg
      121 tggaagatgc aacataggag aattaattaa gctaaaggaa aaaactaatg taaaaatatt
      181 tgtagctact ggaggaaccc tagctagaaa aattataatg gataataaac ctaaagctgt
      241 tgtagctgtt gcatgtgaaa gagatttgac atctggaata caagatatta agaaaattcc
      301 tgttttaggt gtatttaaca aaagacctaa tggaccttgt gtaaatacta atgtagacat
      361 tatggatatt gaaaaagcta taggcttttt aacaggaaaa aatatattag tttgtaatta
      421 aactgttttt aagtgaagtg aatattaaga ggatgtataa taaaaatata ttttaggaga
      481 gtgttattta tgtaccctgt atatggcggt ttttggggat ttgacccaac aatggttgta
      541 ttaattccag ctatattact tactatatat gcacaattta aagtgagttc aacaacaaat
      601 aagtatttaa gagtaaatac acgtcgagga tatactggag agcaaacagc aagaagagta
      661 cttgattcaa atggtttgta tgatgtaaag atagaaatgg ttagagggca tctaagtgac
      721 cattatgacc caagaagaaa ggctgtaagg ttgtcagaag atgtatatta tggaacatct
      781 ataacttctg ttgcagtagc tgcacatgag tgtggtcatg ccatacaaca tgctaaaggt
      841 tatgcacctc ttcaaataag aagtagttta gtaccagtag tgaattttgc ttcaagtata
      901 tcttggtttt taatattttt aggatttatt atggctggtc catttttaaa aattggtata
      961 ctattgtttt cggcttcagt gttattccaa ataataacat tgcctgtaga atttaatgct
     1021 tcaagtagag caatcgttca acttgggaat ttagggatta tagatgaaag tgagtcaaga
     1081 caaagtagaa gagtactttc agcagcagct ttaacatatg ttgcagcagc attggtttca
     1141 atacttcaat tacttagatt gttactaata gctcaaagaa gaaatgacta aaagtaaagt
     1201 gggttgttta gaaacaaccc ttttttatgt ttaataaaag ggagaaattt atatggatgc
     1261 aagagaagtt ggatttaaag tattatgtga tatagaaaaa aatgacaatt attcaaatat
     1321 tgcaataaat aagcatttta aaaatttaga aataagtgat atggatagag gacttgcaac
     1381 agagttaatt tatggagtaa tagaaaataa atattattta gattatataa tcaataaact
     1441 ttcaaaaata aaggtaaaaa aaatgtcaac ttatgttaaa atctttttaa gaatgggaac
     1501 atatcagatt ttatttttag atagtatatc agattatgca gcagttaatg aaactgtaaa
     1561 attatctaag aaatatgata aaaaatcttc tggtttcata aatgctattc ttagaaatga
     1621 aataagagct aaagagacta taatggacat aactgaggaa gatagtgtaa aatacttatc
     1681 tataaagtat tcctataact cttggattat aaaaaactgg atagataaat ttggacaaga
     1741 atttgctgaa gatttgttgg aagcaaataa tgaaaagcct agtatctata taagaacgaa
     1801 tacacttaaa ataagtagag aagaacttat tgaaaaatta agtgaaatgg gtattgtatg
     1861 tttaaaagtt cctatggtag aagaagctat taaagtcgaa aaaatgaaaa atatagaaaa
     1921 aaatgaatta tttaaggctg gtttatttac aattcaagat ataagttcta tgatagtagg
     1981 gaaagtaatt aatccaaaag aaggttcatt tgttttggat gtatgtagtg ctcctggagg
     2041 gaaatctact cacttagcta ctttaatgaa caatactggt caagtagtag ctagagatat
     2101 ttttgagcat aaactaaagc ttataaactc tactgtaaat agattggggc ttaaaaatgt
     2161 gtttgtagaa agatttgatg cttctgagat tgatgaaaat agtataagta aatttgatta
     2221 tgttttagca gatgttcctt gctctggttt tggtataata agacgtaaac cagaaattaa
     2281 gtataagaaa gaagaagagt taaaagacat cacttctata caaaagaaaa ttttagaaaa
     2341 tgcatctaaa tatgtcaaag ttggtggaac tttagtatat agtacatgta caattcaaga
     2401 tgtggagaat ataaatatag ttacttcttt tgttgaagaa aatcacaact ttgaatttgt
     2461 tccaattgat acagtaaatg tagacttaga caatcaagat aaaggatatt taaagatata
     2521 tcctaatatt catggtatag atggtttttt catagcaaaa ttaaagagaa taaggtagtg
     2581 agagaatgga aaagaaaaag atagtattaa aaaattttac tgaagatgaa cttaaagagt
     2641 ttatgaaaac tatagatgaa aagcccttta gaggaagtca aatattctct tggatatata
     2701 agggtgcaaa aacttttgat gatatgaaca atataccaaa aagtttgaga aataaattag
     2761 aagaggtttc atgtataggg catatagata tagaattaaa gttagaatct aaggtagata
     2821 atacaaaaaa atatttgttt ttattagatg atggaaatat aatagaaact gtgatgatgg
     2881 attatgatag tagagttact gtctgtgttt ctaaccaagt aggttgtagg atgggatgta
     2941 atttttgtgc atctacaatg gatggattaa ttagaaactt agagccatgg gaaattttag
     3001 accaagttat taaaattcaa gaagatactg gtaagagggt atcaaatctt gtattgatgg
     3061 ggagtggtga gccacttgat aactttgaaa atacaaaaca atttttaaaa ataattaacg
     3121 aaaaaaatgg tcttaatata gggtatagac atataactct ttcaacttgt ggaatagtac
     3181 ctaaaatgta tgagttggca gacttagaaa tagctataaa tttagcttta tcacttcatt
     3241 caccatatga tgaagaacga agaaaaatta tgcctgtagc aaatgcttat tctattgaag
     3301 aaatattgaa tgcatgtaga tattatataa aaaagacaaa tagaagggtt acatttgaat
     3361 attctcttat aaaaggagta aatgactcag aaaaagaagc aaaggcatta gctaagctcc
     3421 taaaaggtat gctatgtcat gtaaatttaa ttccaataaa taaagttgaa gaaagagaat
     3481 acgaaaaacc agataaagca tttatttata aatttaggga tagtttggag aagaacaata
     3541 tacctgccac agttaggatg tctatgggtt ctgatattag tggagcttgt gggcaattga
     3601 gaagaaaata caagtaattt gtaaggagga agtcgtatgg tttatagttg tgcctcccat
     3661 ataggaaaaa taagaaaaaa caatgaagac tattgtgagg gagaagttat agatacagaa
     3721 catggtccaa taggaatatt tgccatagct gatggaatgg gtggacataa aaaaggtgaa
     3781 gtcgccagta aattagcagt agaaaatata attgattttc taaaagaaaa cttattacaa
     3841 catgataatg taaaaataga ctatatagat gatatattaa aacaggctta taataatgta
     3901 aactcaattg tgcacaaaaa atctatggaa gatatagaat ttgaaggaat gggaactaca
     3961 ttagttacgg ctatagtgta taataatgtc ttatatgtgg caaatgttgg agatagcaga
     4021 tgttatttgt taacagatga aaaatttgat aaaataacta tagaccattc cgtggttgaa
     4081 gaacttatga tggcccatgt cattacagaa gaagaggcaa gaagacatcc acagaggaat
     4141 agaataacta gagcaattgg aacagatgat atggttgttg tagatatttt taagaaagaa
     4201 atcaaaaaaa gtgatataat acttcttgcg acagatggat taactgggtt tattgatgat
     4261 gaagatataa gagatttaat attagattat gaatatgaga gaactagtaa tataagtgaa
     4321 gaattgataa gtatggccaa tgatgtttct ggtaaagata atgtttcggt tatagtgata
     4381 aaagtataat taggacggtg atattgtggg agatacaatt ttaggaaatc gatatgaaat
     4441 catcaggaag attggtgatg gaggaatggc ctttgtatat gaggctaaag acagattatt
     4501 aaacaggact gttgcactta aagtacttag acctgaattt gtagatgatg atgaatttct
     4561 aacaaagttt aaaagagagg cagaagcagt agcaagtctt tcacatccaa atatagtaaa
     4621 tgtttatgat gttggtgaag atggaaaagt tcactacatt gtaatggagt ttgtagatgg
     4681 gaagaatcta aaagagatta tacaagatga aggtatatta gatgaatata ctgcattaga
     4741 tataacaaaa caaatagcta tggcacttag tgcagcgcac aaaaagggga ttatacatag
     4801 agatataaaa cctcataata ttctcatatc aaatgaagga agagtagtaa aagtggctga
     4861 ttttggtata gccaaagctg tttcaaattc aacaatgact aatattggta gtataatagg
     4921 ttcagtgcat tatttttcac cagagcaagc taaaggtaaa tttgtaacta ataatgctga
     4981 cctgtattct ctaggtatag ttttatatga aatgttgata ggaaaagttc catttagggg
     5041 agatagtcca atttctatag cacttcaaca cattaatgat gatatagact ttacatcaga
     5101 agaaaaagtt aggattcctc aaagtgtaag aactacaata aaaaagctta cagaaaaatc
     5161 tagtgcagat agatatcaaa ctgctgaaga attgatagaa gatatagagt atatagaaaa
     5221 aaatattgat ttagatttta taaaagaata tgatgatttt gcaactaaga aaattgatga
     5281 aaaagaaatt aataaagttg taaatccaac acttgcaaaa ccagctcctg aaaaagtggt
     5341 taagcctgtt gaagtagctg atttggatga tgatgaagat tactatgatg atttttatga
     5401 agaagatgat gatgaagatg aagaagagga agagataatg agagctaaaa agaatcagag
     5461 acctaagagt actccaagta agagaactaa gaaaaagaag aaaaaacagg aaagcccaaa
     5521 atctagaaga agattaaaag taattgcggc tgttttgata ctgatattgt gtgctcaagt
     5581 attcttagca tataagtttt tatttgctgg agggtttggc aataaatctt tgactgttcc
     5641 taatcttgta aatatgacat tagaagaagc tcaaagtgct gttgaaaaag aaggattata
     5701 cctgagtgta aaaagtgaag agtataatag tgaagttgat gaaaattgta taatatcaca
     5761 gacacctgaa ggtggaagta caaatgtcaa aaagggtgat actataaatg ttgtcgtaag
     5821 taaaggttca agtcaggcat ctgttcctaa tgtagtggga cttactttga gtaatgcaaa
     5881 acaacttatt gaagaaaata acttaaaagt gggaactgtt aagtatgaat acagctcaat
     5941 atataaagaa ggtacagttt taagtcagag tccgggtgct ggctcatcaa gagctcagga
     6001 aggtgatgag gtaaacctat atgtaagcaa aggttcagaa aaatcaaata cacagactcc
     6061 aacagtacca gataaaaaac caacaacacc agagaataat acaccaacag aacctggttc
     6121 aaactcagga aacagtggtg gaaattcagg aggctcaaat tcaggaaata gtggtggaaa
     6181 ctcaggcaat aatggaagta actcaggagg ttcaaactca ggaaatagtg gtggaaactc
     6241 aggaggctca aactcaggag ataatggagg aagttcagga ggctcaaact caggagacaa
     6301 tggaggaagt tcaggaggtt caaactcagg agacaatgga gatagttcag gaggctcaaa
     6361 ctcaggagat aatggaggaa cttcatcagg aactggcgca aacattggta aaacagaata
     6421 aatattaata aaataagagc tgtcctttta tagaaaatat tttctttaaa gggcagtttg
     6481 ttttgtataa aatactgaat tggttataat ataaggagaa aagtatttat gaagaagaaa
     6541 acagttttaa ttacaggagg agctagaggg attggtaaag ctatgtctaa agcttttgca
     6601 aaagaaggtt acaatgtatt agtaaatttt aataagtcag aaaatgaagc taaagagttg
     6661 tacactatat taaatgaaaa aaatttttct gtaaaacttt ttaaggctga tatttcaaat
     6721 agagaagatg tagaggatat ggtagattat tgtataaaag aatttggagg attggatgta
     6781 cttgtaaaca atgctggtgt aagtcaagat aagttgttta cagatataac tgatgaagat
     6841 tgggataata tgatgaatat taatttaaaa ggaagctttt attgttcaca agttgcttta
     6901 aaatacatga tatcagaaaa aaaaggaaat ataattaata tatcttcaat ctggggtata
     6961 tcaggtgcgt cttgtgaagt gcattattct ataacaaaag caggcataat aggtatgaca
     7021 aaggcattag ctaaagaagt aggaccatca aatattagag taaatagcat agctccaggt
     7081 gtcattaata ctgacatgtt atctggatat aatgaggaag atatagacgc tcttgtggaa
     7141 gaaacccctc taatgaggct aggaactcca gaagatatag caaattgtgc tattttcttg
     7201 gcatcagaca aatcaaattt tatcactggt caggtaatta gtccaaacgg aggatttgtt
     7261 atttaaaaaa aatgtggtat tatataaaaa taggaaagaa taacacgata tactactcat
     7321 aaacagtatg tgtttatata aattttataa agggaggcat ttgatgctag aaggaaaaat
     7381 tataaagggg attagtggat tttattatgt agatacttat aatggtattt atgaatgtaa
     7441 ggctagaggg atatttagaa agcagaaaat aacacctttg gttggtgata gagtcaaaat
     7501 aagcattgtt gatgaaaatg aaaaaaaggg tatcttagaa gaaatcgaca gtagagatac
     7561 agaacttata agacctccta ttgcaaatgt agataaagca ttaatagtat tcgcaataaa
     7621 aaatcctaaa ccaaacttat ctcttttaga tagatttata gttttggcag aaaaagaaaa
     7681 tttagaaact gtaataatcc ttacaaaagc agatttagat gataatgata cattagaaac
     7741 tgttaaaaat atatatgaac ttagtggcta taaagtcatt cctgtaagca atattactaa
     7801 gttaaatata gataaagtaa aagaagaatt aaaagaaaat gtagtagtgt ttgcaggacc
     7861 atctggagtt ggaaaatcaa gcttattaaa tgaaatagat gaaaatttta aacttcaaac
     7921 aggagttgta agtgataaaa taaaaagggg aaaacatact actcgtcacg ctgaactttt
     7981 aaaattagaa tttggtggaa tggttgcaga tacaccaggt tttagttccc ttgcccttga
     8041 agatatagag gaagttgaac ttaaagatta ttttatagaa tttgataaat ttaatgattg
     8101 taagtttggt tcaaagtgta ttcatgaaaa tgaacctaat tgtgctataa aagaagctgt
     8161 tacaaatgga gaaatatcta aagaaagata tgatagttat atacagctgc tacatgaaat
     8221 aagacaaaat aatagtagga ggtattagaa acatgattaa attagcacca tcaatattat
     8281 cagcagattt tgcaaaatta ttagaagatg taagaaaagt agaaagtgca ggatgtgaat
     8341 atcttcatat cgatgtaatg gatggacact ttgtaccaaa cattacatta ggaccacttg
     8401 tagtcaaaag tttaaagaaa gaaaatataa atatggtatt tgatgctcat cttatgatag
     8461 aaaatccaga ccagtatatt gaagaatttg taaaagcagg atgtgatata ataactgttc
     8521 accaggaggc ttgtgttcat ctacatagaa ctatacaaaa tataaaatca catggaataa
     8581 aagctggtgt agtacttaat ccagctacac cagtagatac gattaaacat gtactacctg
     8641 atttagatat ggtattatta atgtctgtta accctggatt tggaggacaa tcatttattc
     8701 cttgtgtttt agataagatt aaagagttaa aagctattat agatagtcaa ggattaaaca
     8761 ttgatataga agttgatggt ggtataagcc ctaaaaacgt agccgaagtt gtacaagcag
     8821 gagctaatgt aatagtagca ggttctgcaa tatttggaag tgatgacatt caagaaacag
     8881 ttaatttatt tagaaagaat gcttctcttg aagaattagt ttaaatttaa taatgaaaat
     8941 ctgtattgtt ttaaatggcg aaattgaaga ttacgaagtt acaagagata ttataattaa
     9001 ggaatgttat gactgcataa tatgtgcaga tggaggggca aatcacactt ataaaatgga
     9061 aataatgcca gattatatat taggagattt agattcagta gaagaagaaa agataaactt
     9121 ctataaaaat aaaggtgtta aatttgaaaa atttccatct aaaaaagatg agactgatac
     9181 agagctttgt ctttttttag ctaagacact taaagcaaat catattgatt tttttggtgc
     9241 tttaggggga aggattgacc acactcttgc aaatataaag ctattatatt atttaaaaga
     9301 agatggtata tattctagga tactttctga taaagaagag atgtatatag ttgaaaatga
     9361 ggaaatttca ttgtatggaa acccagggga cactatttct gtaatagcta taaatggtga
     9421 tgctaagggt gtaacactta ctggcttaga ataccctctc gataattatt atatgaaata
     9481 ctctgtacct attggaatat caaatgttat gttgagtaat tcttgtaaga ttaaagtaga
     9541 gcaaggctgt gtacttgtag ttagaaattt ataaaatttg attttgacaa ttggttttat
     9601 attgtagagc agatacaatc aagtaaaata tatttatgat tgtatctgct ttttagtata
     9661 taattataaa tatataataa actttgacat aagggggatt ttttgtggta gatagtatta
     9721 tttttgattt agatggaaca ttgtgggatt caactgaaga agtatgcaaa gtctggcagg
     9781 atgttttaag tgagcataag gaaatagaat taagtgtcac aaaagattta tttagaagtc
     9841 ttatggggct ttcttttgag gaaatagcaa atcgtttatt tccaaattta agtgaagaag
     9901 aaagaatgaa tttattgaat gaatgttctt tgaaagaatg tgactattta tctgaaaatg
     9961 gagctaaatt atttgatgat atagaagata ctcttaaatt actatctaaa aaatataaat
    10021 tatttatagt aagtaattgt caagctggat atatagaatc ttttttaaaa gcacataaac
    10081 tagaacagta ttttatagat tttgaatgtc ctggtaatac tggtttgcat aaaggagaaa
    10141 ataataaact tataattgaa agaaataaat taataaatcc aatctatgtt ggtgatacgc
    10201 aaggtgatgc aaatagtgct aagtttgtag gtataccttt tatatatgcc aaatatgggt
    10261 ttgggaatgt agatgtatat gattatttta tagattcatt taaagatttg ttagaacatg
    10321 atatattaaa ataagatgag tttaaaaatt atattaaaat aataaaaaag ttatgggggg
    10381 atttaaaaat ggaattacaa gatactattt ttaaaagaca aagtgtaaga aagtttaaaa
    10441 atcaagatgt atcagatgaa gatattttaa aaatgataaa agcagcaggt gcagcaccat
    10501 caggcaagaa tattcaaaac tggcattttg ttgttataaa acgccgtgat ttaatggaaa
    10561 agatagcaga tgtgataact aaaaagcaac aagaaatact tgtagaaatg gataaagtat
    10621 cagtggataa ggctaataga tttagaaaat ttgttaaaaa ttttacacta ttttacttaa
    10681 aagctccagt tttagtatta gtttttacaa aggtatataa tccatcagga tattatgaat
    10741 tagagcttat agatgcacct aaggagacta tagataaatt atttataaga aatccaggta
    10801 tgcagagtct aggtgctgca attgaaaact ttacactatc agcaattgaa cttggatatg
    10861 gttcttgttg gttgacaagt caaaactatg cagcagatga gatagaagct gttttagaag
    10921 cagaaactgg ttttgaaaag ggagagtatt ttttaggtgc aatgttagct cttggagtgc
    10981 cagaggacaa tttgaagagt ccatctaaaa aaccagtaga agaaatatgt acatttataa
    11041 aataaaagtc aaggacattt taaaatcgaa gtaaattaaa aacaaaataa attaaaaata
    11101 aatatttaaa aaattaaaaa gtaggtttta ttgatattaa ttttatattt caaatcattg
    11161 atttaattaa tacaataaga actacttt
//
LOCUS       DAYAPA010000133        11179 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.136, whole genome shotgun sequence.
ACCESSION   DAYAPA010000133 DAYAPA010000000
VERSION     DAYAPA010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11179
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.136"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(<1..618)
                     /locus_tag="ACGI59_003224"
     CDS             complement(<1..618)
                     /locus_tag="ACGI59_003224"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HFG9740998.1"
                     /translation="MTIPSSNKVYPRSNDYQTVYLKNVITRENIEVGDYTIYNDFYND
                     PRDFEKNNVLYHYPINNDKLIIGKFCSIACNAKFLMTSGNHSMKSKSTYTFPIFYEEW
                     KLDINHITDAWDNKGNIIIGNDVWIGYDSIIMSGVNIGDGAIIGTRAVVTNDVPPYSI
                     VGGVPAKIIKKRFNNDTISKLLEIKWWDWPYKKIQSNIKHIQSGNI"
     gene            complement(711..1043)
                     /locus_tag="ACGI59_003225"
                     /pseudo
     CDS             complement(711..1043)
                     /locus_tag="ACGI59_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009271880.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase DNA-binding domain-containing protein"
     gene            complement(1121..1324)
                     /locus_tag="ACGI59_003226"
     CDS             complement(1121..1324)
                     /locus_tag="ACGI59_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFG9740999.1"
                     /translation="MNNTDVPIWEKYTLTIEEASKYFRIGEKKLRKRADENLDSGWVI
                     MNSNRIQIKRKQFEKIVDTLDVI"
     gene            complement(1714..1944)
                     /locus_tag="ACGI59_003227"
     CDS             complement(1714..1944)
                     /locus_tag="ACGI59_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFG9741000.1"
                     /translation="MKPTKEVLPFTIILLAVHGDVNAINVILKHFEHYIIRLSQKTLF
                     DEYGNPYIHVEEETKRMLGTKLITAILRFNLS"
     gene            complement(1946..2353)
                     /locus_tag="ACGI59_003228"
     CDS             complement(1946..2353)
                     /locus_tag="ACGI59_003228"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFG9741001.1"
                     /translation="MVDEKTIEHQFDSFCKTVLRNYARDIYDENKRRNDYLVSLESLS
                     LAELSKLSILDDYDSNYICMVSYDYNIRIEDVLMAQAIGKLTKRKQDIILLSFFFNMT
                     NADIATLMDLAENTIHYHKTNALKELKELMQEH"
     gene            complement(2713..3162)
                     /locus_tag="ACGI59_003229"
                     /pseudo
     CDS             complement(2713..3162)
                     /locus_tag="ACGI59_003229"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(3176..4102)
                     /locus_tag="ACGI59_003230"
     CDS             complement(3176..4102)
                     /locus_tag="ACGI59_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9741002.1"
                     /translation="MSDILSRITNKIGDKNIVDKLSALSKSDLNSLLLEVFDRQANTL
                     TATDILKSYQLNRFTIPSGIDPEEIHALESKLLRKAFNMDIKTIMLSPSAPLGSCSVF
                     GSVDQYNVVSALRGTETLADPSNMLAIIIADKLKRKEENNTIPIHMATTARVIRAQNF
                     VGKGLYSHFGLYCMVSSGIDTGSYTCEKMLLEKHLNYYKDILSEIENVRFSIILRKRN
                     GYKDNDGFFDRMVETIKLIFPNIELSIHNDDVDNNYYKGINFKLSVKQGNETVEIVDG
                     GFVDWTYQMLGNKRERCLISGIGLERFLVAVS"
     gene            complement(4227..5009)
                     /locus_tag="ACGI59_003231"
     CDS             complement(4227..5009)
                     /locus_tag="ACGI59_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
                     /protein_id="HFG9741003.1"
                     /translation="MTKTQQKMIQNVLEFSKRDSRIIGLAISSSYITKSLDEYSDLDF
                     LIVADDKSYEEVIEERLLIVEQFGTLVSAFTGEHVGEPRLIISLYDSPVLHVDFKFTT
                     LDGLLDRVEDSAILYEENNCITEIYSKKEAYFPTPDLQWIEDRFWIWVHYACTKIGRR
                     ELFETIDFLSFLRQSVLAPLIQLLLGKLPRGVRKIELDAPQYIEALEKTVANHEYKSC
                     VNALQATINLYLELREELSQSPIVRKVEAERVALQYFQDLIK"
     gene            complement(5165..5785)
                     /locus_tag="ACGI59_003232"
     CDS             complement(5165..5785)
                     /locus_tag="ACGI59_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFG9741004.1"
                     /translation="MHLIDFMNEDIKKKLVKKTYKKKETILFAEQENEYVILMIDGIA
                     EAFILNVKGNISTIHLYRSGSFFGEMEQFNDGKKPVEIVAFTDCNVYKLHRDDFLNWL
                     RGDFEATKFLIREISSKLVVNAELIEELSSLSVKERLLRCISLHYYRSELNLLTKKQL
                     ASEVNAPIRSINRAINECVQEHLIAYENKRFSVINQREVLKNLPTT"
     gene            complement(5912..6580)
                     /locus_tag="ACGI59_003233"
                     /pseudo
     CDS             complement(5912..6580)
                     /locus_tag="ACGI59_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            6651..7487
                     /locus_tag="ACGI59_003234"
                     /pseudo
     CDS             6651..7487
                     /locus_tag="ACGI59_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020220015.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            8159..8926
                     /gene="aroD"
                     /locus_tag="ACGI59_003235"
     CDS             8159..8926
                     /gene="aroD"
                     /locus_tag="ACGI59_003235"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFG9741005.1"
                     /translation="MKRKVQVKNITIGEGRPKICVPIIGKNKKDIIKEAKELKDACLD
                     IIEWRVDFFENVENIKEVKEVLYELRSYIHDIPLLFTFRSVVEGGEKLISRDYYTTLN
                     KEISNTGLVDLIDVELFMGDEVIDEVVNFAHKKEVKVIISNHDFNKTPKKEEIVSRLC
                     RMQELGADLPKIAVMPQNEKDVLVLLEATNEMFKIYADRPIITMSMSGMGVISRLCGE
                     IFGSALTFGAAKSVSAPGQISFKELNSVLNLLHKSIN"
     gene            complement(9254..9931)
                     /locus_tag="ACGI59_003236"
     CDS             complement(9254..9931)
                     /locus_tag="ACGI59_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9741006.1"
                     /translation="MIVIEGSDKFKIAKEYIDVEYTLFSKVTFRYEKLKFKDNAELEK
                     IKMFKYKNGYIPNKLNLSFGYGFSSYKKQIIRETVDTLRLTEIFSSENIEDIKFIKDG
                     TKKLEISIEKVVKFKRRKKRNYVCCYCPDMYRDIKLDKESINKIYNRKIKIEREVNIF
                     EDEDVIINKKVLKFPKSWTKNMQKYWLSENKYPIHSTVIDDDRYKCCNVQYTKNRVII
                     LYYIYNH"
     gene            complement(10081..10398)
                     /locus_tag="ACGI59_003237"
     CDS             complement(10081..10398)
                     /locus_tag="ACGI59_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFG9741007.1"
                     /translation="MNYIGKRLKEERKKANLTSKEFANMVGVSPWYITQIESGKKNPS
                     LKTFIKFVNILNISADVLIKDITSTGKTYLENDINEELKDLNSRELNLISQIIDIIKK
                     NKI"
     gene            complement(10420..10758)
                     /locus_tag="ACGI59_003238"
     CDS             complement(10420..10758)
                     /locus_tag="ACGI59_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFG9741008.1"
                     /translation="MANIGKIIGDRIKVYRTKLGYSQEFLAEKAGLHPTYIGQIERAE
                     TNTSINIIMKIATALDIPLELLFANIITTEGVDNSVPLECYEMINKLTEKEQIAMLEL
                     IKCIIKYKEI"
BASE COUNT         3717 a         1749 c         1586 g         4127 t
ORIGIN      
        1 aatatttcct gattgaatat gcttaatgtt tgattgaatt tttttatatg gccaatccca
       61 ccatttaatt tctaataatt ttgaaattgt atcattatta aatctctttt ttataatttt
      121 tgcaggtact cctccaacaa tagaatacgg tggaacgtca ttagtaacta ctgctcttgt
      181 tccaataatt gcaccatcac caatatttac acctgacata attatagaat cataacctat
      241 ccacacatca tttccaatta taatgttacc cttattgtcc caagcgtccg ttatatggtt
      301 gatatcgagt ttccattctt cataaaagat gggaaaagta tatgtggatt ttgatttcat
      361 tgagtgattt ccactagtca ttagaaattt tgcattacat gctattgagc aaaacttacc
      421 aattattaat ttatcattgt ttatagggta atgatacaat acattgtttt tctcaaaatc
      481 tcttgggtca ttataaaagt cattatatat tgtgtaatct ccaacttcta tattttccct
      541 tgttattaca tttttaagat atacagtttg ataatcatta cttcttggat ataccttatt
      601 tgaacttgga atagtcatat ttaaatctct cctttaatat atattatgtt tttgactatt
      661 ccactactac aatacatctc ttcacagaat cactccttgt taagaatctt cataagataa
      721 tggcgtcctt ttttagtgtt aagtcggata ttctttctta gtccattctt tttagcgtat
      781 aactgacaaa ctgtcatttt cttaccaata gtgtcaatgc tatcatcaag gtcattttgt
      841 attgcttttt ccttatccct taatgatata tcatcacgtt ttccctttgg gggtttatca
      901 gtaggtacaa gtttccaaga gtaaacaaat tgtggtttac caaatgaata tacatactta
      961 tatgcgtatc ttccatcttt tctctggctc tctccagttc ttaaaattct atctttatta
     1021 tctcgtcttt tttcagttga catgataaga agctcctctc aataaccgaa ttgagcttga
     1081 tacgtcatat gaatattata acatataagg ctcaatttca ttagataacg tcaagtgtat
     1141 caacgatttt ctcaaattgt tttctcttta tttgaatgcg attgctattc ataatgaccc
     1201 agccagaatc aaggttttca tcagcacgtt tacgtaattt tttctctcca atacgaaagt
     1261 attttgacgc ttcttcaatc gtcaaggtat atttttccca aataggtaca tcagtattgt
     1321 tcatataatg tatcactcct ttctatgttc gtagttcaga agtagataga tggctttgat
     1381 aagctccatt ggtatttcaa ccaatcccat tcttatggac agaaccgtgt catacaggtg
     1441 tatcattata ctgtggtaca ggttacggca aaacaaccgt tccaaaggtt attcacaaat
     1501 cactatatag tagctctctt gattaaggtc atggcgtata gtatttgttg gctatctgta
     1561 tcacaaacgt atctatctac tttattcagt tgtcaaagaa cagtcggcat tgatttgcct
     1621 ataaaaaatc acaatagctt gtgggctttt atagacaaat aattagtgat tctccatgta
     1681 ccaaatagct tgatacatgg agagagggcg agattaagac aaattaaatc tcaaaatagc
     1741 agtgattagt tttgttccta gcattcgctt tgtttcttct tctacatgaa tatagggatt
     1801 tccgtattca tcaaataaag ttttctgtga aagacgaatg atatagtgtt caaaatgttt
     1861 aagtattacg tttattgcat ttacatcgcc atgtacagca agtaaaataa ttgtaaaagg
     1921 taatacttct ttagtaggtt tcatttcaat gttcctgcat gagttctttc aactctttta
     1981 gtgcatttgt tttgtgataa tgtatggtat tttctgctaa atccatcaat gttgctatgt
     2041 cagcatttgt catgttgaaa aagaatgaaa gtagaattat atcttgtttt ctttttgtaa
     2101 gttttccgat tgcttgcgcc attaatacat cttcaattcg tatgttgtag tcgtaagaaa
     2161 ccatacagat gtagttagaa tcataatcat ctaaaatact caatttactt aattctgcaa
     2221 gtgacaaaga ttcgagagat accaaataat catttctgcg tttattttcg tcataaatat
     2281 ctctagcata gttacgcaaa actgttttac agaaactgtc gaattgatgt tcaattgtct
     2341 tttcatcaac cataagattt caccctcttt ccatcgcaag gagttaaata accttgctta
     2401 ctcctatcac ggtgggaggg cgttaaatct tcgataaatt tcaaaaaaaa gagaaaaaaa
     2461 tacacactct gcctataggg caaaaatgtg tattctttaa aacgtattta atttatattg
     2521 gttgtaagcc accaccatat tgatgtatgg aaatatgcat tggctagctt ttcataaaaa
     2581 tacaaagatg cataagaaat taatagaggg gaaaaactta ttctgttatt aaaaagtagg
     2641 ttaattctaa taaatgctat tgcggtcgaa ttaaactata tacatggcaa tcaatcatgt
     2701 ttctattctt tattatgaat tgtgacagta ttcttcaatc ttaaagccac atttttccaa
     2761 tactttactt gactctctat tgtaggaata tacattagca tgaattttgt taaggttgta
     2821 tttttcaaag gcaattttgc atatttcttt tactgcattt gttgctatcc ctttgcctca
     2881 atagttccta gcaagccagt aacctaattc agcattttta ttaatagagg tatcttcttc
     2941 aaatgttagg ctgatacttc caatagccct ttcttgataa aaaatagcat agctaagttg
     3001 tttaacatct gaagttgcca aacaaaaact aacaaaatct ctagcgtttt gctccctata
     3061 agggtggggg aagttgtcac gaagattctt tgcaatttct ttatcattcg caaactcaac
     3121 taaatcggta atataagcat aattccactc tataagttcc atttttcttc tccttttatg
     3181 aaacagctac aagaaatctt tctaagccaa taccacttat caaacagcgt tctcttttat
     3241 ttccaagcat ttgataagtc cagtcaacaa atccaccgtc aactatttca acggtttcat
     3301 ttccttgttt gaccgataat ttgaaattga tacctttata ataattatta tcaacatcat
     3361 cattatgaat tgataactct atattaggaa aaataagttt gatagtttca accattctgt
     3421 cgaaaaatcc gtcattatct ttgtatccgt ttcttttgcg tagaataata gagaaacgaa
     3481 cattttctat ttcacttaat atatctttat aatagtttaa gtgcttttct aacaacattt
     3541 tttcacaagt gtaagaccct gtatctatac cagatgaaac catacaatat aagccaaaat
     3601 gtgaatataa acctttacct acaaaatttt gtgctcttat taccctagca gtagtagcca
     3661 tgtggatagg tatggtatta ttttcttctt ttctttttaa tttatcagca ataattattg
     3721 ccaacatatt tgaggggtct gcaagtgttt ctgtccctcg tagagcactt actacgttat
     3781 attgatctac ggagccaaaa acgctacagc tacctaatgg ggcagaggga gaaagcataa
     3841 tcgtcttgat atccatattg aatgcttttc tcaataattt tgattccaaa gcatgaattt
     3901 cttctggatc tataccgcta gggatagtaa atctatttag ttggtatgac tttagaatat
     3961 cagttgcagt taaagtattt gcttgtctat caaacacttc taatagaagt gagttcaaat
     4021 ctgattttga tagagctgat aacttatcaa ctatattctt atccccaatt ttatttgtaa
     4081 ttctacttaa aatatcactc atagttatcc tccatactgt tactttcaaa ataattatag
     4141 aattgttaca acaactaatc taggtcattt gaccaaaaat gaaaaaacac tgaatattta
     4201 ctattcaata tttttttact ttgggtttat ttgattaaat cttgaaaata ctgtaatgca
     4261 accctttcag cttcaacctt tcggacaata ggactttgtg ataattcctc tcttaactca
     4321 agatacaggt tgatagtagc ctgcaatgca ttaacacagc ttttatattc atggttagca
     4381 accgtctttt ctaatgcttc aatatattgg ggtgcgtcca attcaatttt tctaactcca
     4441 cgaggtaatt tacctaagag aagctgtatt aatggggcaa gtactgattg tcttaaaaaa
     4501 gataaaaaat caattgtttc aaatagttct ctacgtccaa tcttagtgca agcgtaatga
     4561 acccaaatcc aaaatctatc ttcaatccac tgtaagtctg gagttgggaa gtatgcttct
     4621 tttttagaat aaatttctgt tatgcagttg ttttcttcat agagaatagc tgaatcttca
     4681 accctatcta gtaaaccatc taaggttgta aacttaaaat caacatgaag aacaggggaa
     4741 tcatagaggg aaattatcaa tcgaggttct ccaacgtgtt cgccagtaaa agcagaaact
     4801 aatgttccaa actgttccac aataagtaat ctctcttcga taacttcttc ataggattta
     4861 tcatctgcta caatcagaaa atccaaatca gaatattcat ctaacgattt tgtgatataa
     4921 gaactgctaa ttgcaagtcc tattatccta ctatctcttt ttgaaaattc taatacgttt
     4981 tgtatcattt tctgttgtgt tttagtcata atataacacc tctcactgtc ttttattagc
     5041 tatcaatcca acataattat gcataggagt attatataat gaagaaataa aaaaaaatag
     5101 gtcacttgac ccgaaataaa aaagcattga ataattatta ttcaatactt ttttgaaatt
     5161 atacttatgt tgtaggaaga ttctttaata cctctcgctg attaattaca ctaaatcttt
     5221 tgttttcata agcaattaga tgttcttgta cacattcatt tatagcacga ttaatgcttc
     5281 taattggagc gtttacttcg ctagcaagct gttttttggt aagcagattt aattcacttc
     5341 tataatagtg taaggatata cacctcagaa gtcgttcttt aacacttaaa gatgataatt
     5401 cttcaattaa ttcagcattt acaactaatt tactagatat ttctcgaatc agaaattttg
     5461 tagcttcaaa atctcccctt aaccagttta aaaaatcatc tctatgaagt ttatatacat
     5521 tacaatctgt gaaagcgaca atttcaacgg gttttttccc atcattaaat tgttccattt
     5581 ctccaaaaaa actaccactt ctataaagat gaatcgttga gatgtttcct ttcacattta
     5641 agatgaatgc ttctgcaata ccgtcaatca taagaattac atattcattt tcttgctcag
     5701 caaaaagaat agtctctttc tttttatacg tttttttaac aagcttcttt ttaatatctt
     5761 cattcataaa atcaattaaa tgcatattca tccctcctta ccaaatcttt gattaaatta
     5821 tatcatcttt caattagtta ggtaagatag taaaaatata tttacataga aactattcca
     5881 ttattatttt ccagttgtca tttttctgca aaaacttatc actgtcacga ctggagatac
     5941 cagtaaggaa tgttaggata tcaatcgcta aactttcaga atccttttta cggctcaaaa
     6001 tgacgtaagg gtcaagcaat cctttgtttt catcattact ggttaaattg acaatgttta
     6061 tctcatgggc tatttctggt agcgtttctt tccatttgcc gcgttctgct ttagggtcaa
     6121 ctatgaccgc ctgtccgccg aatagtaccg catagtaaac aagcatattg ttactgaata
     6181 atttaccacc acccaatgaa ccgataaacg cagacgctaa cgcattggta atagaaccct
     6241 ttaccccttg actagcaagg gcaggtttta gatagacatt ctttccagtg tccagattgt
     6301 agccaagata gatatcctca tgttcgccaa gggtttgtgt cgctccaaaa ccaagtccag
     6361 ccaaaaaatc cgaagtgaca tattgaatat aatcattgat gtaacgtttg ctgacaggaa
     6421 taaattcgcc atgcaatccc aacatatccc caaagggacg cacgagcttg attgataagt
     6481 cgtcgtaaaa gtctttgact tcatcacaac gcctttttag ctcgtcaaga ttctttgtag
     6541 atacacggac aacatagcta agtttataca tagattcttt acagttaaaa gtctgttttt
     6601 aaaaaaatgt ataaattata aatcaaaata tagatactat caatgaccca ctaggtgcaa
     6661 aaagcattga tatggttaaa ttatcagacg ctaaaagctg ggctatccga atgagtgaag
     6721 atgggtgtgc ttataaatcc attgacaatt ataagcgctc actgaaagcg tcgtttttca
     6781 tggcaattca agacgactgt attagaaaga atccatttaa tttttagtta agtgatgttt
     6841 tggaagatac aacagaacca aaaatcgctc taacaccaga acaggaaatg ggcttattat
     6901 cctttatggg gagcgataag acttattgta aatattatga tgatgttgtt atcctcttag
     6961 aaacaggact acgcatttct gaattatgcg gtttgacttt agaattggat atgaaaaata
     7021 aagccattat ggtagaccat caattgttga gtgatactaa gattggctat tatgtagaag
     7081 ttccaaagac cacacagggt aagcgagaaa ttccaatgac tgaaagagct tatcaatcat
     7141 ttgaacgtat cgtggaacgg cgtaagaacg ctaaagcaga accaatcatt gttgatggtt
     7201 accagaactt cctatttctt aaacaagatg gattaccaaa acaagcaact cattatcaaa
     7261 gcatgttaag agcgttagtc aagaagtata ataagaccca tgaggacaag ctaccaaaga
     7321 ttacgccaca tacctttagg catacactct gtacaaatat ggctaacaaa ggaatgagtc
     7381 ctaatatatt gcaatatatt atggggcata aaaacattac tatgacgctt ggctattatg
     7441 cacatggttc atttagttct tcgagagctg aaatggaacg gttataatcc tagtagtatg
     7501 actagagttt actacttatt tactactttt gatggcattc ttatacggtt aaatgccaac
     7561 ttatgcaaag tatcttccaa aaagaaaatg ccgatactcc ttgtagatac aggatatatc
     7621 gacatttacc aacttatgaa aagataatca aatttaatat aagtttttca aataaaaaga
     7681 taccaaattc tttttcgaga atctatataa cattacttaa gcttggttgg ggtataaaca
     7741 attgagtagc tacttttgta tggtgttcaa ctttagtcaa aactcttaag taatgcaaat
     7801 gatttagatt cattaatatt caatctcttc ataagtagtg aaattcgagt tatttctgca
     7861 taattatact atgatttata gattaattct attagcatcc agaataatat tgtttatatg
     7921 agtaaaattt aaagaatatt gtgaaaatta taggatgtta aaataaattt aggtaaaaaa
     7981 gaagtgtttg tatattatag agtacctttt ctaaaatatg tgggattgtt ttaagaatta
     8041 gaataaaaat tatttttaaa ttagtatttt tataatgtat agtattttta atgtataatt
     8101 aaattgtgca gaaaatagaa tttaaagtcc tatagaattt ctgattatgg aggagaatat
     8161 gaaaagaaaa gttcaagtaa aaaatattac tattggagaa ggtagaccta aaatttgtgt
     8221 accaattatt gggaaaaata aaaaagatat tataaaagag gctaaagagt taaaagatgc
     8281 atgtttggat attatagagt ggcgagttga tttttttgag aatgtagaaa atattaaaga
     8341 agttaaggaa gttctctatg aattaagaag ttatatacat gatatccctt tattattcac
     8401 atttagaagt gtagtagaag ggggagaaaa attaatatca agagattatt atactacttt
     8461 gaataaagaa atttctaata caggtttagt agatttaata gatgtagagt tatttatggg
     8521 agatgaggtc atagatgaag tagttaattt tgctcataaa aaagaagtga aagtaattat
     8581 atctaatcat gattttaata aaactccaaa aaaagaagag attgtatctc gtttatgtag
     8641 aatgcaggaa ttgggcgcag atttacccaa aatagctgtt atgccgcaaa atgaaaaaga
     8701 tgtgttagtg ctacttgaag ctacaaatga aatgtttaaa atttatgcag atagaccaat
     8761 aataacaatg tcaatgtcag gaatgggtgt tataagtaga ttgtgtggtg aaatatttgg
     8821 ttctgcattg acttttggag ctgcaaaaag tgtttcagca ccagggcaaa tatcatttaa
     8881 agagttaaat tcagtactta atctattaca taagagtatt aattaaatta attatataga
     8941 aatattagac ggtatttttg aagatataaa ttaaatataa aaaacttttc agatagtaaa
     9001 atttaaattg ggcaattata tctgaaaagc aactatttag tgtgtatttt gaaagtgata
     9061 tagaaaatct tataaatcaa ctatggcata tttaaaatat gccattaaaa cctactagac
     9121 attagattat agatggtata ctagcatttc tattatttat agatagtaag ttttcataat
     9181 aactgtttta tctaaagtag aagatatatt tgtattatgt atatttgtca ttattttttt
     9241 gtaattatgt atattaatgg ttatatatgt aatataatat tatcactcta ttttttgtat
     9301 attgtacatt acaacattta tatctatcat catcaattac agtagaatgt atgggatact
     9361 tattttcact taaccaatat ttttgcatat tctttgtcca agactttgga aacttcaata
     9421 ctttcttgtt tattataaca tcctcatctt caaaaatatt aacttctctt tctattttta
     9481 tttttctgtt gtatatctta ttgatagatt ctttgtcgag ttttatgtct ctatacatat
     9541 cagggcaata gcaacaaaca taatttcttt tttttcgacg tttaaatttc acaactttct
     9601 ctatgctaat ttctaatttt tttgtaccat cttttataaa tttaatatct tctatgttct
     9661 cgcttgaaaa aatttctgtc aatcttaaag tatctacagt ttctctaatt atttgctttt
     9721 tataagaaga gaatccatat ccaaaagaaa ggtttaattt attagggatg tagccatttt
     9781 tatatttaaa catcttaatt ttttccaatt cagcattatc tttaaatttc aacttttcat
     9841 acctaaaagt tactttgcta aaaagagtat attctacatc aatatattct tttgcaatct
     9901 taaatttatc gctaccttca atcacaatca tagcctatct ccaatcgttt tatattaaaa
     9961 taaggccata gaacatggtc taatagtact cattagcact ataagacaat gttaacacaa
    10021 aaagacaaaa aaaggttatt tatttcaaat aacctttttt tttatataaa atataaagaa
    10081 ttatatttta ttctttttta tgatgtctat aatctgtgat atcagattta gttctcttga
    10141 atttaagtct tttaactctt catttatatc attttctaaa tatgtttttc cagtggaagt
    10201 aatatctttt ataagaacat ctgcagaaat atttaatata tttacaaatt ttatgaatgt
    10261 tttaagactt ggattctttt taccactttc gatttgagtt atataccaag gcgatactcc
    10321 aaccatattt gcaaattctt ttgaagttaa atttgctttt ttccgttctt ctttaagtct
    10381 tttacctata taattcatta ttgttactat ttccctcact tatatttctt tgtatttaat
    10441 gatgcacttt attaactcta gcattgcaat ttgttctttt tcagttaatt tattaatcat
    10501 ttcataacat tctaaaggta cagagttatc aacgccttct gttgtaataa tatttgcaaa
    10561 taacaattca agtggtatgt ctaaagcagt agcaattttc attataatat tgatagatgt
    10621 atttgtctca gctctttcaa tctgaccaat atatgttgga tgtaaacctg ccttttctgc
    10681 tagaaactct tgactatatc ccaattttgt tctatagact tttattctat ctcctattat
    10741 ttttcctata tttgccaatt attatccctc cactttagat tatattcata gtttaagttg
    10801 ttttatatag actatttata cttttttagt ctaaatatag actatatatg tttttatact
    10861 gaaataagtg ctatttattt ttgtatagtc tgtatctaca ataaacaaat atggataaat
    10921 catattttta attagagaaa tattttcata atttcatagc catactacaa taataaaacg
    10981 aattaataat atgttaatta tagcattttt tattgtaatt acatattaga tatgtagaat
    11041 atattttgaa aattaaaaaa attttctatt ttatatattg aacaatttat gtgaatagaa
    11101 aatttttcaa taagactttt gagtttaatc attatattgg taaggtaaac atttttaaat
    11161 ctaattatat ataggtgaa
//
LOCUS       DAYAPA010000134        10985 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.138, whole genome shotgun sequence.
ACCESSION   DAYAPA010000134 DAYAPA010000000
VERSION     DAYAPA010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10985
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.138"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            <1..1248
                     /locus_tag="ACGI59_003239"
     CDS             <1..1248
                     /locus_tag="ACGI59_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9741009.1"
                     /translation="EIEIYIYRDFSHVGDLYLEIEDRFDGFAVSGPIPKKAITKKAGT
                     IKKPLVDFGTDLQSYYDMFLKLIFKYNTLDFSRAYFDLTDWADNPKDISYYLSNGTFG
                     DLMDSIDENASKMSLSDIAKLEKKIAKKHINLWNEGKIDFSTTRFSSIVPMLQNAGVN
                     FHFIYPDIDLLKNTFNNLIKDIHLHRMKGNQPSVIYITVKDTNYDDSTLDQVYKILNS
                     IKKDKLTDFIIEKNSNSFKIFTKCETIKSLTKNFTACYIKESIENSTNIKVCIGYGIG
                     NDIKQAMSSAISANKESKINPLNYSFLVNENYETIGPLLCDKLLTVSNKVTPHINTIA
                     KSSKLSTLTIQKIISVTEILQRDEVTPKELSSCLNVTVRTANRFLSSLLKSGNATILY
                     EKQNSTKGRPERVYKLFISENKY"
     gene            complement(1376..2566)
                     /locus_tag="ACGI59_003240"
     CDS             complement(1376..2566)
                     /locus_tag="ACGI59_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFG9741010.1"
                     /translation="MKNFFKEAQSIEGEIIRWRRYLHENPEIGCNLPKTVTFVTSTLE
                     KMGYSPKLTSNNGIVAVLDSENGGKTLLLRADMDALPIREETDLPFKSKNKDASHSCG
                     HDNHTAMLLGAAKILMDNKHLLKGRVKFVFEPDEETTRGAKSMIDDGILENPQVDAAM
                     AFHSMVGKFLNTGQVAYSRGPAMASADIFEITVEGKATHGASPEFGVDPINIMSHIQI
                     ALNTILSREKPQKEPVVLTIGMFNAGNVPNAIPNKASMSGTLRTFDQFVREKLKNRII
                     EISTGIAKSFGGNAKVEFKMGTPAVINDLDVGDEIVSYVKEVIGNENVIDFPSAMGSE
                     DFSEVLLRVPGVFLWIGMGSISQGYNFGMHNPKAIFNEEGLVYGSALFAHCAVKWLEN
                     NFSR"
     gene            complement(2672..4051)
                     /locus_tag="ACGI59_003241"
     CDS             complement(2672..4051)
                     /locus_tag="ACGI59_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFG9741011.1"
                     /translation="MIGSISINEIDKKRFKLDDLSKKIWENPEKAFKEFKACENTANF
                     LRSEGFDVEVGVGGLATAIRASFGSGKPVIGFMGEFDALPGLNQKVSTKQEAFELGAY
                     GHGCGHNLLCTAHVGAVVGLKREMIENNLSGTIVFYACPGEEQLTGKGFMARGGAFEG
                     LDLAINFHPNKISEATIGTSTAVNSVKFHFKGKTAHAGSDPQNGRSALDAVELTNVGA
                     NYLREHVTSDVRIHYTITDGGVAPNIVPDKASVWYYTRALSREAVESTYERLVKVAKG
                     AAMMTETEVEVEFLGGCYNTLNNHVLANLVSECMNEIKQEPWTREELDFAAELDRQSP
                     QQYKAMISKYNLPEGTHLYYGGGNVTNFNSYGSTDIGDVMHIVPTAYFFTGCTNLGAP
                     GHSWQFTSCAGSSIGEKGMIYASKIMAMFGAKILNNPEIAKKAKEEFDKSMNGKTYKC
                     PIPDDIPTP"
     gene            complement(4082..5290)
                     /locus_tag="ACGI59_003242"
     CDS             complement(4082..5290)
                     /locus_tag="ACGI59_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9741012.1"
                     /translation="MENFWNPLTCFTVCVMIYAFSEFLSKKTKGAISTLLFVCIIFLL
                     GFWTNILPKDITGASGILAAMSNFGIALLITNLGTLINLEDLCHEWKTVVIALVGIVG
                     IGVGCLTVGSMLFGREYALIAAPPIAGSTVAGIIVTTVAEAANRPELAAFVILVLSFQ
                     KFFGIPITTYCINKELKFKRDNGDFLKDESKSNFKLPSMRIFKEDLGKKSSTLVYLAK
                     LSFVAMIGNFVGLATLIPGFTPANYYLNPNIACLLVGLIFTRIGFLEKGILDKSQSNG
                     IIMFGCMLMLPGGLAKVTPTALFGMIVPTIGILVVGSVFIILFTAIVGKLLGYSPRMS
                     AAIGVTCMLAYPATQIISNEGIDAMEGTEEEKQRATDYLLPKMIIGGFVTVTIASVAF
                     ASIIGPIIFG"
     gene            complement(6315..7769)
                     /locus_tag="ACGI59_003243"
     CDS             complement(6315..7769)
                     /locus_tag="ACGI59_003243"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFG9741013.1"
                     /translation="MNIKDTFFWGGSIAAHQCEGSWDSDNKGPAIMDFVTKGSYEMPR
                     VITDKIEEKLDYPSHNGIDFYNRYKEDIALFKEMGFSALRISIDWSRIFPNGDDENPN
                     ELGIKYYEGLIDTLIENNIEPIVTLYHFELPMNLVHKYGSWNNRKLIDLYLKYSETVI
                     RRFDDKVKYWVTFNEMNHIDPQTEHSDIFTYMIAGIKYSEIENKVQTLANVGYNMTLA
                     GVKVAKLAREINPNNKVGCVFGLNPIYPYNCNPSNVLNAFLDNDRDYYQIDAMCNGKF
                     PQYKLKEYEKNNITIEVTDEDKKAFKEGKLDFIGLNYYFSSVSTPKENLSGDKSLFGG
                     IQNPYLEQSKWGWAIDPIGLRFTLNYIYRKYQLPILITENGLGAYDKIEEDGTINDDY
                     RIEYLGKHLSQMKSAIEEDNVECFGYLMWGPIDLVSATTGQMSKRYGFIYVDLDDEGK
                     GSFKRYKKKSFDWYKEVIKSNGEVLDFSTDIVVG"
     gene            complement(7789..9660)
                     /locus_tag="ACGI59_003244"
     CDS             complement(7789..9660)
                     /locus_tag="ACGI59_003244"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFG9741014.1"
                     /translation="MKYKDLNENIIKLIGGKENIKSVAHCVTRLRFTLNDRSIAQTEE
                     IKKLKGVIDVVSNDVAYQIIIGTHVVDVYNEFMSMIGLSSSESSIKSERKKGIRGIKG
                     IFNSIFVVVSETMTSVIEVLLAAGILASILALLNMSGILQSDSPTYQILDTLRSATFH
                     FLPVLIAIASAKRLNVNPYIAVVLAVTLLSSSIDGVEGLSLFGINLQSTTYANSFIPI
                     LLGVWAMGIIIEGLKKILPKALHYFLVPVLSLIITLSVTLTVFGPIGMWIGDGLNYAC
                     MFLIDTLGNWSVVALYAALQPLLIVFGAANFTMPISLNSVTTLGYDPIFMGAATISDI
                     AVCGAMFGYFLKTRNKEQKQLFGTVSFSALMGVTEPSVFGVFMKYRRPFVAVMIGGGI
                     GGLIAGLAQVKTYGMVWGLAALPTYLVGGEVSNFTFMIISVVVSFVAATAASYLLGIP
                     SDEKEEVKGDNEKELELIINENNSRTISIGNVAKGQVIALGEVNDKAFSSGALGKGVG
                     VIPSESKSTVISPVDGTITIVFPTKHAYGIVTDEGLEILIHIGIDTVNLDGKFFESLV
                     TQNQRVKKGDALAVFESDKIIEEGFDSTIITVVTNTSDYLDVISSNDESEELLTVIV"
     gene            complement(9650..10510)
                     /locus_tag="ACGI59_003245"
     CDS             complement(9650..10510)
                     /locus_tag="ACGI59_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFG9741015.1"
                     /translation="MKIIQILNNNVALVKRGKNEIIVFVKGIGFKKRVGQVINENEIE
                     RTYILDSYDMLEHFSYLLQNSDSNNIVLINEIIEYGEKTLNIKASDYLSLTLLDHLEF
                     LLKRCKKNQFIKSPLVWNVKRFYPKHFEIGMHALKLIGDSKGINLPDDEAVSIALHFV
                     NMEVNKESHDSTIVELRTLADIVSIIKYHFNVELDETSTNYMRFTTHLQYFIQRIMSE
                     EASIDEDNSIDLYKQVSQLYPKSFGSVQKIRKYIKSQFNVDISISEETYLMLHINRVT
                     QRLEAKSDEI"
BASE COUNT         3679 a         1988 c         1230 g         4088 t
ORIGIN      
        1 gaaattgaaa tatatatcta tagagatttt tctcatgttg gagatttata tttagaaata
       61 gaagataggt ttgatggatt tgcagttagt ggccccatcc ccaaaaaagc tattacaaaa
      121 aaggctggaa ctattaaaaa acctctagtt gattttggta ctgacttaca aagttactat
      181 gatatgtttc taaagcttat ctttaaatat aatacattag atttttccag agcatacttt
      241 gacttaacag actgggcaga taatccaaaa gatatatcat attatttatc aaatggcaca
      301 tttggagact taatggatag tattgatgaa aatgcatcta aaatgagttt atctgacata
      361 gcaaaacttg aaaaaaagat tgcaaaaaaa catattaatc tatggaatga agggaaaatc
      421 gatttttcta ctactcgttt tagtagtatt gttcctatgt tacaaaatgc aggagtaaac
      481 tttcatttca tataccctga tatagattta ttaaaaaata cttttaataa cttaataaaa
      541 gatattcatt tacatagaat gaagggaaat caaccaagtg taatatatat aacagttaaa
      601 gatacaaatt atgatgactc tactctagac caagtttata aaatattaaa tagtattaaa
      661 aaagataaat taaccgattt tataattgaa aaaaatagta attcttttaa aattttcact
      721 aaatgtgaaa cgattaaatc attaactaaa aattttactg catgttatat aaaagaatct
      781 attgaaaata gtacaaatat taaagtatgt attggatatg gaattggtaa tgatataaag
      841 caagctatgt ctagtgccat aagtgctaat aaggaatcta aaataaatcc tttaaattac
      901 agtttcttag ttaacgaaaa ttatgagaca ataggacctt tactgtgtga taagttactt
      961 actgtttcaa ataaagtaac tccacatata aatacaattg ccaaaagttc taaattgtct
     1021 actcttacta ttcaaaagat tatatctgtt actgaaattc tacaaagaga tgaagttact
     1081 cctaaggaat tatcttcatg tttaaatgta acagttagaa ctgctaatag atttctaagt
     1141 agtctcttaa agtctggaaa cgctactata ttatatgaaa aacaaaactc aacaaaaggc
     1201 agacctgaaa gggtatacaa attatttata tcagaaaata aatattaaaa tccaaaatgc
     1261 ataaaaatat tttatcatct aatattaagt aaaattatat atgactttct aaaaggtata
     1321 tctttgcatc tttttaagat atacctttta tgatatttaa taatatttta tgttattatc
     1381 tactaaaatt attttctaac cattttactg cacaatgagc aaataaagct gaaccataaa
     1441 ctaatccctc ttcattaaaa attgctttag gattatgcat accaaaatta tatccctgac
     1501 taatacttcc cattccaatc cataaaaata ctcctggaac ccttagtaaa acctctgaaa
     1561 aatcttctga acccattgca gatggaaagt ctattacatt ttcatttcct ataacttctt
     1621 taacataaga tacaatctca tctcctacat ccaaatcatt tataactgca ggagtcccca
     1681 ttttaaactc cactttagca tttcctccaa atgatttagc tattcctgta gatatttcaa
     1741 taattctatt ttttaatttt tctctaacaa attggtcaaa tgttcttaaa gttcctgaca
     1801 tagatgcttt atttggtata gcatttggta cgtttcctgc attaaacata ccaatagtta
     1861 aaactacagg ctctttctga ggtttttctc ttgaaagtat agtgtttaat gctatttgta
     1921 tatgcgacat tatgtttatt gggtccactc caaattcagg gcttgctcca tgagttgctt
     1981 ttccttctac tgttatttca aatatatccg cagatgccat agctggacca cgtgaataag
     2041 ctacttgacc tgtgttaaga aatttaccca ccatactgtg aaatgccata gctgcatcga
     2101 cctgaggatt ttctaatata ccatcatcaa tcattgattt tgcacctctg gtagtctctt
     2161 catctggttc aaatacaaac tttactctac ctttaagtaa gtgcttatta tccataagta
     2221 ttttagctgc tcctaagagc attgctgtgt gattatcgtg tccacaacta tgagatgcat
     2281 ctttattctt acttttaaaa ggtaaatccg tctcctccct aataggaagt gcatccatat
     2341 ctgcccttaa taataaggtc tttccaccat tttcactgtc taaaacagct actattccat
     2401 tattagacgt caatttcgga gaatatccca ttttttctaa agtactggta acaaaggtta
     2461 ctgttttcgg tagattgcaa ccaatttcgg gattttcatg taaatacctt ctccatctaa
     2521 taatttcccc ttctattgac tgcgcttctt taaaaaaatt tttcatattc cttcctccta
     2581 atcagatagt cagcaaaatc tcactttctt ttagtaatga aatgatgctg atataaaaat
     2641 tataatttta tatattaaat ttcatgtctt tttaaggagt tggaatatca tctggaatag
     2701 gacatttata tgttttacca ttcatagatt tatcaaattc ttcttttgcc tttttagcta
     2761 tctctggatt atttaatatt ttagctccaa acatagccat aatctttgat gcatatatca
     2821 tacctttttc tcctatagaa cttcctgcac aagatgtaaa ctgccagcta tgacctggtg
     2881 ctcctagatt cgtacaccct gtaaaaaagt atgctgtcgg aactatatgc attacatctc
     2941 caatatcagt agagccataa ctattaaagt ttgtaacatt tcctccacca taatatagat
     3001 gagttccttc tggcaaatta tatttactta tcatagcctt atattgttgt gggctttgtc
     3061 tatctaattc agctgcaaaa tccaattcct ctcgagtcca aggctcttgt ttaatctcat
     3121 tcatacattc tgatacaaga tttgctaaaa catgattgtt taaagtatta taacaccctc
     3181 ctaaaaactc tacttcaact tcagtttcag tcatcattgc agctcctttt gctaccttta
     3241 caagtctttc atatgtactt tctacagctt ctctacttaa agctcttgta taataccata
     3301 ctgatgcttt atcaggaact atgttgggtg ctactcctcc atctgtaatt gtataatgaa
     3361 tccttacatc agaagtaaca tgttctctca aataattagc tcccacatta gtaagttcca
     3421 cagcatctag tgcacttctt ccattttgag ggtctgaccc tgcatgtgca gtctttccct
     3481 taaagtgaaa ctttacagag ttaacagctg ttgaagtacc aattgtagct tcactaattt
     3541 tatttggatg aaaatttata gctaagtcta acccttcaaa cgctccacct ctagccataa
     3601 atcctttacc tgttaattgt tcttctcctg gacatgcata aaatactata gtcccactta
     3661 agttattttc tatcatctct cttttaagcc ctactactgc ccctacatgt gctgtacata
     3721 ataaattatg cccacatcca tgaccatatg cacccaattc aaatgcttct tgctttgttg
     3781 aaactttttg atttaaacca ggtaatgcat caaattctcc cataaaacct ataactggtt
     3841 tacctgaacc aaaactagct ctaatagccg tggctaaacc tcctacacct acttccacat
     3901 caaatccttc acttcttaga aagtttgctg tattttcaca tgctttaaat tctttaaatg
     3961 ctttttctgg attttcccat attttcttac ttaaatcatc aagtttgaat ctttttttat
     4021 ctatctcatt tatagatata cttcctatca tttcctattc ctcctataag tatatttact
     4081 atcaaccaaa tataattgga cctattatac tagcaaatgc cactgatgca atggttacag
     4141 taacaaatcc tcctataatc atctttggta ataaataatc tgttgccctt tgtttttctt
     4201 cttcagttcc ttccatagca tcaatacctt cgttggaaat tatctgtgtt gcaggataag
     4261 ctagcataca agttacacca attgcagctg acattcttgg agaatatccc aatagttttc
     4321 ctacaattgc agtaaataat attataaata cagaacctac aacaagtatt cctatagttg
     4381 gaacaatcat cccaaacaat gctgttggag ttaccttagc aagtcctcca ggtaacatca
     4441 acatacaacc aaacattata attccattag attgagattt atctaaaatt cctttttcta
     4501 aaaaacctat tcgtgtaaaa atcaacccta ctaacaaaca tgctatatta ggatttaaat
     4561 aatagtttgc aggggtaaat cctggtatta aagtagccaa tcctacaaaa ttaccaatca
     4621 tagcaacaaa tgaaagcttt gcaagataca ctagagtaga tgatttttta cctaaatctt
     4681 ctttaaaaat tctcatactt ggaagtttaa aattactttt tgactcatct ttcaaaaaat
     4741 ccccgttatc tcttttaaat tttaattctt tatttataca atatgtagtt atcggaatac
     4801 caaagaactt ttgaaatgat aatactagaa ttacaaatgc agcaagttca ggtctatttg
     4861 cagcttctgc aacagttgtt acgataatcc cagcaacagt agaacctgct attggaggtg
     4921 ctgctattaa agcatactct ctgccaaata acatactacc cactgttaaa cagccaactc
     4981 ctattcctac aatacctact aatgcaatta caaccgtttt ccattcatga cataagtctt
     5041 caagatttat aagtgttcct aagttagtta ttaataaagc tataccaaag ttgctcattg
     5101 cagctaatat ccctgatgct cctgtaatgt cttttggtaa aatatttgtc caaaatccta
     5161 gtaaaaatat aatacataca aataacaatg ttgaaattgc accttttgtt ttcttagata
     5221 aaaactcact aaaagcataa atcattacac atacagtaaa gcaagtaagt ggattccaaa
     5281 aattttccat tgttgtttcc tcctaaatat aataattttt tatcaataaa acaataatta
     5341 taaaatgtat attatgcagt tattcttaac tacatatggt atagcttgat attttttgat
     5401 gatattatat acgtcctaaa ttattcctaa aatatctaaa taaatataac tagagttaac
     5461 tctctaaata ttaactatag ttatcaactt actagatttc tttttgattt ttactttcta
     5521 cataatttta cgagttagaa ttgtacttca actactctct atattagtat acatgttgga
     5581 agacttatat ttatttaaat attagatata gaatacattt ttataaacgt cttaaattat
     5641 tcttaaaata tctaaaacga aataataata tacccattaa accaatatta tcagaatata
     5701 cttcacttta agtcttcttt atatagaaat tttatctatt agtgcatccc ctcctttgta
     5761 aattagacta tttctaaacg aatttagata tcttattcct aggaactatt actagaaaca
     5821 aaaattattt attatattaa atcaatgata agccatactt taaaattctt tctatacaag
     5881 tataatttat catataatga catctatacc tttatcttta tagtagtcct aaattattct
     5941 taaattgaca ataaaaataa tttcactatt tataaaaata cttgttttta tattgtcaaa
     6001 ctattcttaa tatagattat aattttaaat cttttctttg tcaatatatt ctgacatttt
     6061 tccagaaaat ttataaagtt atatagtgag taaatcatac tatttttata taatgagtaa
     6121 attatactat gaatatgtct aaatatttac ttaagtataa acataaaaaa tgtgtattct
     6181 cctttttata gtaattttag ttgccattac ttttaagaag aatacacact ttactttaaa
     6241 gaccctatac tctattttaa atgctttact agacactaat taacattagt tcctaacttc
     6301 tatttatctt attcctaccc tactactata tccgtagaaa aatctaaaac ttctccatta
     6361 gattttatta cttctttgta ccaatcaaat gattttttct tatatctctt aaaacttcct
     6421 ttgccttcat catctaagtc aacatagata aatccatatc tcttactcat ctgacctgta
     6481 gttgcactga ctaaatcaat tggtccccac atcaaatatc caaaacattc aacattatct
     6541 tcttcaattg cagacttcat ttgagataga tgcttaccta aatattcaat tctataatca
     6601 tcatttatag ttccatcttc ttcgatttta tcatatgccc ctaaaccatt ttctgtaatt
     6661 aagataggta attgatactt tctgtaaata tagttcaagg taaatcttaa acctataggg
     6721 tctattgccc acccccactt actttgttcc aaatatggat tttgaatccc accaaataaa
     6781 gatttatcac ctgataaatt ttcttttgga gtagatacac ttgaaaaata ataattaagt
     6841 cctataaaat ctaacttacc ttctttaaat gcttttttgt cttcatcagt cacttcaata
     6901 gttatattat ttttttcata ttcttttaat ttatattgtg gaaacttccc attgcacatt
     6961 gcatcaattt gataataatc acggtcatta tctaaaaatg catttaacac attactagga
     7021 ttacaattgt atggataaat tggatttagt ccaaatacac atccaacttt attgttggga
     7081 ttgatttctc tagccaactt tgcaaccttt acacccgcca aagtcatatt ataccctaca
     7141 tttgccaatg tttgaacctt attttctatt tcactatact taatacctgc tatcatataa
     7201 gtaaaaatat ccgagtgctc tgtttgaggg tcaatatggt tcatctcatt aaatgtaacc
     7261 cagtacttta ccttatcatc aaatcttctt attacagtct cactgtactt taaatacaaa
     7321 tctataagtt ttctgttatt ccatgaacca tatttatgta ctaaattcat aggtaattca
     7381 aaatgatata gtgttacaat aggttctata ttattttcta ttaatgtatc tatcagacct
     7441 tcatagtatt taatccctaa ttcattagga ttttcatcat ctccatttgg aaaaattctt
     7501 gaccaatcta tagatattct taatgcacta aatcccattt ctttaaataa agctatatct
     7561 tctttatatc tattataaaa atctattcca ttatgagatg gataatccaa tttttcttct
     7621 attttatcag taattactct tggcatttca taagagccct tagttacaaa atccataatt
     7681 gcaggtcctt tgttgtcact atcccatgaa ccttcacatt gatgggctgc aatacttcct
     7741 ccccagaaaa atgtatcttt aatattcatg aattttccct cctaaaattt atactattac
     7801 agttaataat tcttctgatt catcatttga tgagattaca tctaaataat cgctagtatt
     7861 tgtaactaca gttattatag tagaatcaaa gccttcctct ataattttat cagattcaaa
     7921 tactgctaaa gcatctcctt ttttaaccct ttgattttgt gttacaagac tttcaaagaa
     7981 ttttccatct aagtttacag tatctatccc aatatgaatt aatatttcta atccttcatc
     8041 agtcactata ccatatgcat gtttagttgg aaatactata gttatagttc catctacagg
     8101 agatataaca gttgattttg attcactagg tataactcca acccctttac ctaatgctcc
     8161 agaggaaaat gctttgtcat taacttcccc aagtgcaatt acctgacctt ttgcaacatt
     8221 tccaatacta atagttcttg aattgttctc gttgattatt agttctaatt ctttttcatt
     8281 atcaccctta acttcttctt tttcatctga aggtattcct agtaaatagg aagctgctgt
     8341 tgctgctaca aatgatacta ctacacttat tatcataaat gtaaagttag atacctctcc
     8401 tccaacaaga tacgttggta atgcagctaa tccccaaacc attccatatg ttttaacttg
     8461 agctaaacct gctattaaac cacctatacc tccacctatc attactgcta caaatggtct
     8521 tctatatttc ataaacacac caaacactga aggttctgta actcccataa gtgcactaaa
     8581 acttactgta ccaaataatt gtttttgttc tttattccta gtttttaaga aatatccaaa
     8641 cattgcacca caaactgcaa tatcagatat tgttgcagcg cccataaaaa ttgggtcata
     8701 ccccaatgtt gtcacagaat ttaaagatat aggcatagta aagtttgctg caccaaagac
     8761 tattaagagt ggttgtaaag ctgcatacag tgcaacaaca ctccaattac ccaatgtatc
     8821 aattaaaaac atacatgcat agtttaatcc atcaccaatc cacataccaa taggtccaaa
     8881 tacagttaat gtaactgata gagttattat taaagataat actggaacta aaaaatagtg
     8941 tagtgcttta ggtaatattt ttttcaaacc ttcaattata attcccattg cccatactcc
     9001 taaaagtatt ggaataaaac tgtttgcata agttgtagac tgtaaattta taccaaataa
     9061 acttaatcct tctacaccat ctattgatga agataataac gtaactgcta atactactgc
     9121 tatatatgga tttacattaa gtctttttgc tgatgctatt gcaattaata ctggtaagaa
     9181 gtgaaatgta gcacttctta atgtatctaa tatttgatat gtaggactgt cactctgaag
     9241 tatgccagac atatttaaaa gtgctaatat acttgcaagt ataccagctg caagtagtac
     9301 ttcaattact gaagtcatag tctctgagac taccacaaag atggaattaa atataccttt
     9361 gatacctcta attccctttt ttctttcact ttttatactt gattcacttg aacttaaacc
     9421 tatcatagac ataaactcgt tatatacatc tacaacatga gtacctatta taatttgata
     9481 agcaacatca ttagaaacta catcaattac tccttttaat tttttaattt cttctgtttg
     9541 agcgatactc ctatcattta aagtaaatct taaacgagta acacaatgcg ctaccgactt
     9601 tatattttct tttcccccta taagttttat tatattttca tttaaatctt tatatttcat
     9661 ctgattttgc ctccagtctt tgagtcaccc tatttatatg caacattaaa taagtttctt
     9721 cacttataga tatatcgaca ttaaattgtg attttatata ttttcttatc ttttgaacac
     9781 ttccaaatga ctttggataa agttgactta cttgtttata taaatctata gaattatctt
     9841 catctattga agcttcctct gacataattc tttggataaa gtactgtaga tgagtagtaa
     9901 atctcatata atttgttgaa gtttcatcta gctctacatt aaaatgatat tttattatag
     9961 aaactatatc tgctaaagtt ctcaactcta caattgtact atcatgagac tctttattta
    10021 cctccatatt tacaaaatga agtgctattg atactgcctc atcatcagga agattaattc
    10081 ccttactatc accaattaat tttagtgcat gcattccaat ttcaaaatgc tttggataaa
    10141 atcttttaac attccatacc aaaggacttt ttataaattg attcttttta catcttttaa
    10201 gtaaaaactc taaatggtct agtaaggtta agcttagata atcacttgcc tttatattta
    10261 aagttttctc accatattca attatttcat taataagaac tatattattg gagtctgagt
    10321 tttgtagtag ataactaaag tgttctagca tatcgtatga atctagtata tatgttctct
    10381 ctatttcatt ttcatttatt acttgtccca ctcttttttt aaagccaatt cctttaacaa
    10441 atacaataat ttcattttta cctcttttta ctaaggctac attattattt aaaatctgaa
    10501 ttattttcat atattcctcc cttcaataaa aagaactcaa gcatatatgt atagagtatc
    10561 cctctatttt tcacatatat acttgagttc tgcctgctaa tcagtaacat actcaaaatt
    10621 atgactacgt cgccataatt aaaattcttt tttgtttaat ttcatattct atatgttata
    10681 ctatccatat aaatctgtca acggtttcct agatattttt ttattctata actacaagct
    10741 ctgttatgcg attatctgac aaggtttata tactaaattg atactatttt accgtcacta
    10801 gtatttataa tattgtctga accattatct attttactat tatgaattat catagtaatg
    10861 cttttaaata taatgaactg tattttaaca aataaattct taagatatat taacatataa
    10921 agtcttaagt ttagttttat ttaatcacta ttaagatata ttaataggta aagcttcaaa
    10981 tttag
//
LOCUS       DAYAPA010000135        10812 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.139, whole genome shotgun sequence.
ACCESSION   DAYAPA010000135 DAYAPA010000000
VERSION     DAYAPA010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10812
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.139"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            <1..955
                     /locus_tag="ACGI59_003246"
     CDS             <1..955
                     /locus_tag="ACGI59_003246"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFG9741016.1"
                     /translation="SMKYELLEKEIIHKEELRLSNECYKIIVEQTDSVVFEWNIKENK
                     SFVSQAWTEKFGYNNACKNIFKEIKDKDLVHSEDKAIFEGFLESIKKKNMHNQAVYRL
                     KKSNGEYIWCRTSITSIYNDENELLRVVGVIVDVDSDIKKYEELRTRAESDSLTNIYN
                     KGTFEKLVEETIVMNTGDKKDALFIIDLDDFKEINDNFGHPFGDFVLKTFADKIQTSF
                     GSKDLVGRIGGDEFVVYMQDYVTEVNLHKKAKELNRVLSDNYTDLSFSFDASVSIGIA
                     RYPQDGTSFFELFKNADRALYSIKASGKNSYCLFEEELYVQ"
     gene            complement(1318..2148)
                     /locus_tag="ACGI59_003247"
     CDS             complement(1318..2148)
                     /locus_tag="ACGI59_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFG9741017.1"
                     /translation="MSQVSKNYFTTGEFAKICGINKKTLFHYDDIGLFSPELKKENGY
                     RYYSYHQLSIFGIISSLREVKMPLKEIKAYIDKRTPNLLIELLEKKTIDIKNEIEKLN
                     NIQALMESTISFTKSACNIDANTITLKEHEEEYLVKTPVTYKEQFLGDEEENFLYECI
                     NFMDNYELSDYGTIGSIIKGEDIINKDFESYSYLFTKVNKEYKKYPVSIKPKGLYVTA
                     YHKGSYETIYKAYEKLLNFFNQNNLRIGDFVYEEYLLYDISVRDSNEYLTQISAEVKM
                     "
     gene            2221..3948
                     /locus_tag="ACGI59_003248"
     CDS             2221..3948
                     /locus_tag="ACGI59_003248"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9741018.1"
                     /translation="MKKLIHFLKPYRVLIVVVLIFTFLQTLGTLYIPTLTANIVNNGV
                     VKGDIDYIVKTGLMMMIVAGITALSAVLVCKVSANLSSGFCRDIREAVFIKSQDLSIN
                     DFNNIGTASMITRSTSDITLIGQSVFMFIQLVLPAPIITVSGLFLAYSIDKAMTIIIV
                     VVMFLFMLSAFLVGKKLIKLFKMMQIKMDNMNRVLREVVTGVRVIRAFNRSRFEKKRF
                     DRTAIDYSETAISINKIFAVLMPIVMLIMNLGIVSIIWFGGMRVSNGNMEIGHIMALV
                     EYCILILFYLIMGVMVFMYIPRAGACADRVNQILDIEPEIVDGNGHKDTVSERGHLVF
                     KNVTFSYAQSEEPVLNNITFEAKSGEVTAIIGSTGSGKSTIANIIPRFFEIQSGEISI
                     NGQDIKKIPQKELRDKIGFVPQKAFLFSGTIEENIRYGKEDASIEEVKHAASIAQADE
                     FIYDMEDKYDSFVAQGGNNLSGGQKQRISIARALVRKPEVYVFDDSFSALDFKTDKRL
                     RKALKNEIKDSSAIIIAQRISTIMDANQIIVLNDGKIVGIGKHKDLLENCEVYKQIAD
                     SQLSKEELA"
     gene            3985..5847
                     /locus_tag="ACGI59_003249"
     CDS             3985..5847
                     /locus_tag="ACGI59_003249"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9741019.1"
                     /translation="MSEKRIDEQDIEVLSFEDGMSAEKAKDAKKTTKRLLKYMAKQKF
                     KILIIFISVLISSALTVLAPMVMGKAIDQLFNGIKTAVQTGTKFSVNFSTMGGIVSIL
                     LGLYLISAVFIYIQQYIMSGVAQNLVLSMRKDLSDKLNKLPLKYYDSHKKGETLSIVT
                     NDLEKVADSLQEGLMQLITAVVTVVGSIVMMISISVPLTIVSAITLLVSLGITVVIAR
                     KSQERFSENQKALGELNSNIEEIFTGQIVVKAFSKEKDTIRNFKILNQNLYNASRKAQ
                     FSSYAISPIIRFINLIGYVIIAVVGGIFASTGAMTLGSIQAFIQYVNQASEPTTEISY
                     IVNMLQAAIASAERVFTVMDEVEEIKDEESSKVISMPKGKVQFEHVKFGYSDDFILMK
                     DININLNAGDKIAIVGPTGAGKTTLVNLLMRFYEIQGGRITIDGVNIKDLKRGELRTM
                     FGMVLQDTWLFNGSIKENIAYSKSDATMDEIVRAAKSARVDHFIRTLPQGYDTILTED
                     ASNLSQGQKQLLTIARAILSDPSVLILDEATSSVDTRTEVEIQKAMNNLMKGRTSFVI
                     AHRLSTIRDADLILVMKEGTIIEQGSHKELIEKKGFYEELYNSQFTSEYDEDVV"
     gene            6180..6869
                     /locus_tag="ACGI59_003250"
     CDS             6180..6869
                     /locus_tag="ACGI59_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HFG9741020.1"
                     /translation="MNKKLNYRFEMKHKITEADVLALKSRLYPIMKKDENASKDGKYL
                     IRSLYFDTPEDKALLDKLNGVAIREKFRIRFYNNDCSYIRLEKKIKHYNMTSKLSTNL
                     TKKEVINILNNDIEFLKESTNCLLREFYLKLKCERLEAKSIVDYNREAFIYPVGNVRV
                     TIDSDIKTSVNSVDLFNKDLPTISVIDENMTVLEVKYDEFIPDFIKDLIQINKTTSTA
                     VSKYASSRLYI"
     gene            6986..7660
                     /locus_tag="ACGI59_003251"
     CDS             6986..7660
                     /locus_tag="ACGI59_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HFG9741021.1"
                     /translation="MTFNDIFKSSFIENVSGFSFVDSALALGSAFLVGLFIYMVYKKT
                     YMGIMYSRPFNVSLVALTMLTTFVILAVTSNVVLSLGMVGALSIVRFRTAIKDPMDLV
                     FLFWSLGSGIVLGAGLIPLAVMGSIIMGLILIFFSNKTISETPYILMVNCNNEDSEDI
                     ATDKIKKVFTKYQIKSKSVTPEKGIELVFEVRMKDGETSLINDLSQVDGVTNAVLVSY
                     NGDYVA"
     gene            7677..9584
                     /locus_tag="ACGI59_003252"
     CDS             7677..9584
                     /locus_tag="ACGI59_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HFG9741022.1"
                     /translation="MKDKKFTLLISIMIIFLCAVVGVYSTSSNKSADLYSDVYIEKYF
                     NRDKVMEVNIEIDESDLKDMNENAIKEEFKVAKVTVDGDTYGNVGIRTKGNSSLISVA
                     NSDSDRYSYKINFDKYNTSQSMEGLTQLNLNNCYSDPSYMREFLTYSICEEMGLATPE
                     FAYAKVSINGEYHGLYLAVEGLKESYLENNFGNVTGDLYKSDEGSSLQYKGDDPESYS
                     NLIVESDKKTADWSKITKLLKSLDTGEDIEKYLDVDSVLKNIAINTALLNLDSYQGSF
                     AHNYYLYEQDGVFSMLPWDFNMSFGGFSGFGGGSQSIAIDEPTTGNLEDRPLISSLLK
                     NETYKTKYHKYLEEIVTKYLDSDYLENMTTKLHDMIASYVKEDPTAFYTYEEFEKNIT
                     SSIEDSSDNKGFGNKGFDNNNSNNSDSNNNSNSENKRSGNQSDEKEVNAELTSSVVKA
                     NTDNETKNKTTNDSESKNNTDKDKSGNDNNQKLEGPMGKGGKSIPGVLEVAEDMSKTI
                     KSQLSGETSSTKQNSGDESSSGIKGSEKFDEDMSGMPEPPEGMDGKMPPGMGNMDKGD
                     MNGKNGNMNMDRNQDNPREAGGFGNRGGGSVSKTTTYFKLILGGASMIIMSIMLVGVS
                     RVKRRRFIKSK"
     gene            10037..10726
                     /locus_tag="ACGI59_003253"
     CDS             10037..10726
                     /locus_tag="ACGI59_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFG9741023.1"
                     /translation="MLQKVLWKFLKLVVGLFICSVGIVLTINCNLGLSPWDVFHQGLS
                     NHIGITIGTASIIVGSIVVIADVVLGENVGWGTVFNMLLIGFFMDLLLYSNLIPEADS
                     LFVGIIMLILGLVLLSVGMVFYMGSGLGSGPRDGLMVAIQKKTGKSLKLIRGTIEVGA
                     LIVGFLLGGKVGIGTIISAFGLGYFTQMVFGLFKLDCSKIKHRFIVDDIKFIKVYMSG
                     EKKAAVQNIVK"
BASE COUNT         4163 a         1119 c         1916 g         3614 t
ORIGIN      
        1 atctatgaaa tatgaactac ttgaaaaaga aataattcat aaggaagaat taagacttag
       61 taatgagtgt tataaaatta ttgttgaaca gactgattct gtcgttttcg aatggaatat
      121 caaagaaaat aaatcatttg tatcgcaggc ttggactgaa aaatttggat ataacaatgc
      181 atgtaagaat atttttaaag aaattaaaga taaagactta gttcattctg aagataaagc
      241 tatatttgaa ggttttcttg aaagtattaa aaaaaagaat atgcacaatc aagcagtata
      301 tcgtttaaaa aaatctaatg gtgaatatat ttggtgtaga acatcaatta caagcattta
      361 taatgatgaa aatgaacttt taagagttgt cggagtaatt gtagatgtag atagtgatat
      421 aaaaaaatat gaagagttaa gaactagagc agaaagtgac tcattgacca atatatacaa
      481 taaaggtaca tttgaaaaat tagtggaaga aactatagtt atgaatacag gtgataaaaa
      541 agatgcatta ttcattattg atttggatga ttttaaagaa ataaatgata attttggtca
      601 tccatttggt gattttgtac taaaaacatt tgctgataag atacaaacta gttttggtag
      661 taaagactta gtcggaagaa ttggtggaga tgaatttgtt gtatatatgc aagattatgt
      721 tacagaagta aatttgcata aaaaagctaa agaattaaat agagtacttt cggataatta
      781 cactgatttg agttttagtt ttgatgcttc tgtcagtata ggtattgcaa ggtatcctca
      841 agatggaact tcattttttg aattgtttaa aaatgcagat agagcacttt atagcataaa
      901 agcatcaggt aaaaattcat attgtttatt tgaagaagaa ctatatgtac agtgaatttg
      961 aatttagatt ttatgtgaga gtaagattta tatatacttg tggctgtctg atttataaaa
     1021 tatgttactt tgtatatttt tatatgtttc agtaaggtta attaacatga gcaaaggtgt
     1081 aagtaaattt atattagttt aagtttactt acacctttat aaaaatacaa ataatagatt
     1141 catttacatt cttaaatgat taaagctctt taccttctaa acatgcataa catatcgtta
     1201 taattccagt aaataagact aaaatgccca ttactaagat tacaatttca tcatctatta
     1261 ctccaaatgt ttttaaagta acttatttcg ctttgtatca taataatttg tctcttttta
     1321 cattttaact tctgctgaga tttgagttag atattcatta gaatctcgca cacttatatc
     1381 atatagaaga tattcttcat aaacaaaatc cccaatacgt aaattattct gattaaaaaa
     1441 atttaataac ttttcataag ccttgtaaat agtttcataa ctccctttat gataagcagt
     1501 tacataaagt ccctttggtt tgattgatac aggatatttc ttgtactctt tattaacttt
     1561 tgtaaataaa tatgaatatg attcaaagtc tttattaatt atatcttcac ctttaatgat
     1621 tgagccaatc gtaccataat cactcaattc atagttgtcc ataaagttaa tacattcata
     1681 taaaaaattt tcttcctcat ctcctaaaaa ctgttcttta tatgtaacag gtgttttgac
     1741 caagtactct tcttcatgtt cttttaaagt aatagtattc gcatctatat tacatgcact
     1801 tttagtaaat gaaatagtag attccattag agcttgaata ttatttagtt tttcaatttc
     1861 atttttaata tcaatggttt tcttttctaa aagctcaatt agtaaattag gtgttctttt
     1921 gtctatataa gctttaattt cttttaaagg catttttact tccctaagtg aagaaattat
     1981 accaaaaatg ctaagttgat ggtatgagta gtatctatat ccattttctt tctttaattc
     2041 tggtgagaaa agacctatat catcataatg gaaaagtgtt tttttattga ttccacaaat
     2101 ctttgcaaat tctccagttg taaaataatt ttttgaaact tgactcataa ttataattcc
     2161 tccttgacta tcctgttaca gtatagttta tcatagtatt ttgtaaagga gtgtgattat
     2221 atgaaaaaat taattcattt tttaaaacca tatagagttt taattgttgt agttttgata
     2281 tttacgtttt tacaaacact tggaactttg tatataccaa cacttacagc aaatatagta
     2341 aataatggtg tagtaaaggg agatatagat tatattgtaa aaacaggttt aatgatgatg
     2401 atagtagctg gtataacagc tttatcagct gtgttagttt gtaaagtatc agcaaatctt
     2461 tcatctggat tttgtagaga tattagagaa gcagtattta taaaatcaca agatttatca
     2521 ataaatgatt ttaataatat aggtacagca tcaatgatta ctaggagtac tagtgatatt
     2581 actttaatag gtcagtctgt gtttatgttt attcaattag tattaccagc accaattata
     2641 actgtttctg gtctattctt agcatattct atagataaag caatgacaat tattattgtt
     2701 gtagtaatgt ttttattcat gctttcagca tttttagttg gtaaaaagct tataaagttg
     2761 tttaaaatga tgcaaataaa gatggataat atgaataggg tgttacgtga ggtagtaact
     2821 ggagtaagag ttataagagc ttttaatagg agtcgttttg aaaaaaagag atttgataga
     2881 actgcaatag attattctga aacagcaatt tcaataaata aaatatttgc agtacttatg
     2941 cctattgtaa tgttaataat gaatttaggt atagtaagta ttatttggtt tggtgggatg
     3001 agagtcagta atggaaatat ggaaataggt catataatgg cacttgttga atattgtatc
     3061 ttaattttat tctatttaat catgggagta atggtattta tgtacatacc tagagcaggt
     3121 gcatgtgcag atagagtaaa tcagatatta gatatagaac ctgaaattgt agatggaaat
     3181 ggtcataaag atactgtctc agagagagga catttagtat ttaaaaatgt aaccttttca
     3241 tatgcacaat cagaagaacc tgtgttaaat aatataacat ttgaagcaaa atctggagaa
     3301 gtaactgcaa ttataggaag cactggctca ggcaaatcta ctattgcaaa tataatacca
     3361 cgtttttttg aaattcaatc tggagaaata tctataaatg gtcaggatat taagaagatt
     3421 cctcaaaaag aacttagaga taagattgga tttgtaccac aaaaagcttt tttatttagt
     3481 ggaacaatag aagaaaatat aaggtatgga aaagaagatg caagtattga agaagtaaag
     3541 catgctgcaa gtatagctca agctgatgaa tttatttatg atatggaaga caagtatgac
     3601 tcatttgtag cacaaggtgg aaataatttg tcaggtggtc aaaaacagag aatatccatt
     3661 gcaagagcgt tagttagaaa gcctgaagta tatgtctttg atgatagttt ttctgcatta
     3721 gattttaaaa cagataaaag acttagaaaa gcgttgaaaa atgaaatcaa agattcatca
     3781 gctataatta tagcacaacg tataagtaca atcatggatg ctaatcagat tatagtttta
     3841 aatgatggaa aaattgtagg tattggaaaa cataaagatt tacttgaaaa ttgtgaagtt
     3901 tataagcaaa ttgcggattc acaacttagt aaagaagaac ttgcataaaa tgaagaatta
     3961 atctgatatt tggaggtgta tgatatgagt gaaaaaagaa tagatgaaca agatatagaa
     4021 gtacttagtt ttgaagatgg catgtcagca gaaaaagcta aggatgcaaa aaaaactact
     4081 aagagattat taaaatatat ggcaaaacaa aagtttaaaa tattaattat ttttatttca
     4141 gtattgataa gttcagcgct tacagtttta gcacctatgg ttatgggaaa agctattgac
     4201 caactattta atggaataaa aactgcagtc caaacaggta caaaattcag tgtgaatttt
     4261 agtactatgg gagggatagt atcaatactt ctagggttgt acttaattag tgcagtattt
     4321 atatatattc aacagtatat tatgtcagga gttgcacaaa atctagttct tagcatgaga
     4381 aaagatttaa gtgataaact caataaactt cctcttaagt actatgattc tcataaaaag
     4441 ggggaaacac ttagtatagt aacaaatgat ttagaaaaag tagcagatag tttacaagaa
     4501 ggattaatgc aacttattac agcagtagtt acagtagttg gttctattgt aatgatgata
     4561 agtataagtg taccacttac aatagtatca gctataacat tacttgtttc attgggtatt
     4621 actgttgtaa ttgcaagaaa atcacaagaa agattctctg aaaatcaaaa ggcactagga
     4681 gagttaaata gcaatataga agagatattt actggtcaaa tagtggttaa agcatttagt
     4741 aaagaaaagg atactataag aaactttaaa atattaaatc aaaatcttta taatgcaagt
     4801 agaaaagcac agttttcgtc atatgcaata tctccaatta ttagatttat aaacctaatt
     4861 ggctatgtta ttatagcagt tgtaggtggg atatttgcat caactggagc aatgactctt
     4921 ggtagtatac aagcgtttat tcaatatgta aatcaagcat cagagcctac tacagaaata
     4981 tcttatattg taaatatgct tcaagcagca atagcatctg cagagagagt ttttacagtt
     5041 atggacgagg tggaagagat aaaggatgaa gaatcttcaa aggttattag catgcctaaa
     5101 ggtaaagtac aatttgagca tgttaaattt ggatatagtg atgattttat tcttatgaaa
     5161 gatattaata tcaatctaaa tgcaggagat aagattgcaa ttgttggacc tacaggagct
     5221 ggaaagacaa ctcttgtaaa tttacttatg agattttatg agatacaagg tggaagaatt
     5281 actattgatg gtgttaatat aaaagattta aagcgtggag aacttagaac tatgtttggt
     5341 atggttcttc aagatacatg gctgtttaat ggaagtataa aagaaaatat tgcatatagt
     5401 aagtcagatg ccacaatgga tgaaattgta agagcagcaa aatcagcaag agtagaccat
     5461 tttataagga ctttaccaca agggtatgac actatattga ctgaggatgc ttctaatcta
     5521 tctcaaggtc aaaagcaact tctaacaatt gccagggcaa tactttcaga cccatcagta
     5581 ttgattcttg atgaagcaac atctagtgta gatactagaa cagaggtaga aattcaaaag
     5641 gcaatgaata acttgatgaa gggaagaact agttttgtta ttgcacatag attatcaact
     5701 attcgagatg ctgatttaat attagtaatg aaagaaggaa ctattattga acaaggaagc
     5761 cataaggaac ttattgaaaa gaaaggattc tatgaagaac tttataacag tcaatttaca
     5821 agtgaatatg atgaagatgt tgtatgatta tcaagtttta ggcaactaaa tgattgataa
     5881 atattaagtt aaatatagta acatgtaagg ttatatggaa taagagtatt tctctatttc
     5941 atataacctt ttttatttat caaaataaga taaaatttcc tttctaaatt agattgaaga
     6001 atgtatttat caatataaaa gtaaaataat cattattatt agtgaagtaa atattgtttg
     6061 ccacatatat gccataaaga tgcacttatt ttacggagat ttaacaaagt agtgtaaagt
     6121 atactctaaa tataaaataa atcaaataga gttaaataaa ttgagaggtg atataaatag
     6181 tgaataaaaa attgaactat agatttgaaa tgaagcacaa aataactgaa gcagatgtgt
     6241 tagctttaaa atcaagactc tatccaataa tgaagaaaga tgaaaatgca tctaaagatg
     6301 gaaaatatct tattaggagt ttatattttg atactcctga agataaagca cttttggata
     6361 aattaaatgg cgttgcaata agagaaaagt ttaggataag attttataat aatgactgct
     6421 cttatataag gttagagaaa aaaatcaaac attacaacat gacatcaaag ctatcaacta
     6481 atttaaccaa aaaagaagta ataaatatac taaataatga tattgaattt ctaaaagagt
     6541 cgacaaattg tttattaaga gaattttatt tgaaattaaa atgtgaaaga ttagaggcaa
     6601 aaagcatagt tgattacaat agagaagctt ttatttatcc agttggaaat gtaagagtta
     6661 caatcgatag tgatattaaa acttctgtaa attcggtaga tttatttaat aaagatttgc
     6721 caactatttc ggtaattgat gagaatatga ctgtattgga ggttaaatat gatgaattta
     6781 taccagattt tataaaggat ttaattcaaa ttaataagac tacctctaca gcagtttcaa
     6841 agtatgcttc aagtagatta tatatttaag aataaaggct atgatttata taaagatata
     6901 cataaataag gttaaaaaac aaataagtta aaaataaata aaataaaaaa taaataagtt
     6961 aaaaatagga aggatgattt taattatgac atttaatgat atttttaaat caagttttat
     7021 agaaaacgta tcaggttttt catttgtgga cagtgcttta gcactaggtt cagctttttt
     7081 agtaggtttg tttatatata tggtctataa aaagacttat atgggaatta tgtattctcg
     7141 accattcaat gtgtcgcttg tagcacttac aatgcttaca acttttgtaa tattggcggt
     7201 tacttcaaat gtagttttat ccttaggtat ggttggtgct ttatctatag taagatttag
     7261 aacagcaatt aaagacccaa tggatttagt atttctattt tggtcattag gttcaggtat
     7321 agttttagga gcaggtttga ttcccttagc tgtcatgggt tcaataatta tgggattgat
     7381 tctaatattc ttttcaaata aaacaatatc tgaaactcca tatatattga tggtaaattg
     7441 taataatgaa gattcagaag atatagcaac tgataaaatt aaaaaagtat ttactaaata
     7501 tcaaataaaa tcaaaaagtg ttacaccaga aaaaggaata gaattagtgt ttgaagtaag
     7561 aatgaaagat ggagaaacaa gtcttataaa tgacttgagt caagtagatg gagttactaa
     7621 tgcagttctt gtaagttata atggtgacta tgtagcatag agaggagtaa tcttttatga
     7681 aagataaaaa atttaccctt cttatctcga ttatgattat atttttatgt gctgtagttg
     7741 gagtttatag tacatctagc aacaaaagtg ctgatttata tagtgatgta tatattgaaa
     7801 aatattttaa cagagacaag gttatggaag ttaatataga gatagatgaa agtgacttga
     7861 aggatatgaa tgaaaatgct ataaaagaag aatttaaggt tgcaaaagta actgtagatg
     7921 gagatacata tggaaacgta ggtataagaa ctaaaggaaa ttcaagtctt atatctgtag
     7981 caaatagtga tagtgataga tacagctata agattaattt tgataagtat aatactagtc
     8041 aaagtatgga agggcttact caattaaatc ttaataactg ttactctgac ccatcttata
     8101 tgagagagtt tttaacatat agtatttgcg aggaaatggg attagcgact ccagaatttg
     8161 catatgctaa agtctctata aatggcgaat atcatggttt gtatttggca gtagaaggat
     8221 taaaagagtc ttatcttgaa aataattttg gtaatgtaac tggagactta tataagtcag
     8281 atgaaggaag ctcgttgcaa tataaaggag atgacccaga aagttactca aacttaatcg
     8341 ttgaaagtga taaaaagaca gctgattggt ctaaaatcac aaaactatta aaatctttgg
     8401 atacaggtga agatattgaa aaatatcttg atgtagattc tgtccttaaa aatatagcaa
     8461 taaatacagc tttattaaac cttgatagct atcaagggag ttttgcccat aactattatt
     8521 tatatgagca agatggagta ttttctatgt taccatggga ttttaatatg tcatttggtg
     8581 gatttagtgg ttttggtgga ggtagtcaat ctatagcaat tgatgaacct acgacaggta
     8641 atttagaaga cagacctctc atatcctcgt tattaaaaaa tgagacatac aaaacaaaat
     8701 accataaata tctggaagag atagtaacaa aatacctaga ttcagactat ttagagaata
     8761 tgacaacaaa attgcatgac atgatagcat catatgtaaa agaagaccca acagcatttt
     8821 atacttatga agaatttgaa aaaaatataa catcttcaat tgaagattct agtgataata
     8881 agggatttgg taataaaggg tttgacaaca ataactctaa taacagtgat tctaataata
     8941 attctaatag tgaaaataag cgctctggaa atcaaagtga tgaaaaagaa gttaatgctg
     9001 aattaacatc aagcgtagtc aaagctaata cagataatga aactaaaaat aaaactacaa
     9061 atgatagtga aagtaagaat aatacagata aagataaaag tggaaatgat aataatcaaa
     9121 agctagaagg tcctatgggt aaaggaggta agtcaatacc aggggttttg gaagttgcag
     9181 aagatatgag taaaactata aaatctcaat taagtggaga aacttcttcg acaaagcaaa
     9241 actctggtga tgaaagttca agtggaatta aaggtagtga aaagtttgat gaggatatga
     9301 gtggtatgcc agaaccacct gagggaatgg atggtaaaat gccaccagga atgggtaata
     9361 tggataaggg agatatgaat ggtaaaaatg gcaatatgaa tatggataga aatcaagata
     9421 atccaagaga agctggaggt tttggcaata gaggaggagg ctctgtgagt aaaacaacaa
     9481 catacttcaa attaatttta ggtggagctt caatgataat aatgtcgatt atgttagttg
     9541 gtgtatcaag ggtaaagaga agaagattta taaagtcaaa ataagattaa aattgaatat
     9601 aaataatttt aatactgtta agtctaaata tttacttagc agtatttttt tagaaaataa
     9661 tgtatttggt taataatata aggtaataaa ttgataaaaa ttaaatacag catattaata
     9721 atacataata aagtaataaa aaaactgttt aagtataata acttatattt agacagtttt
     9781 tttcatttat aaataatata aatgaaattg aaaacatttg aaggatagaa attggttgga
     9841 taaatgtata aattacttca ccaaaaaata atgacaaaaa ttgtaactaa gttgtaataa
     9901 aatggtagtt atttagcata aaataatgta tcatagaagt atattataaa attaataaaa
     9961 taagattttt gtaagaaaac tgctggcttt tgatatttag agtgagtagt taaattatgt
    10021 ttaggagggg tagaatttgt tgcaaaaagt attatggaaa tttttaaagc tagtagtcgg
    10081 tttatttata tgttcagttg gtatagtatt gacgataaat tgtaaccttg gattatctcc
    10141 ctgggatgta tttcatcaag gactttcaaa tcatatagga ataactatag gaactgcttc
    10201 tataattgta ggctctatag tagttattgc tgatgtcgtt cttggagaaa atgtaggatg
    10261 gggaactgtt tttaacatgc tactaattgg attctttatg gatttacttt tatatagtaa
    10321 tttaattcca gaggctgata gtttatttgt aggaataatt atgttgatat taggattggt
    10381 tcttttgtct gttggaatgg tattttatat gggaagtggt ttaggaagtg gacctagaga
    10441 tggtttgatg gttgcaattc aaaagaaaac aggaaaatca cttaaattaa taagaggtac
    10501 tatagaagtt ggagcattaa tagtaggatt tttattaggt ggaaaagttg gtataggaac
    10561 tataataagt gcatttggat tggggtattt cacacaaatg gtatttggct tatttaaatt
    10621 agattgtagt aagattaaac atagatttat agtagatgat ataaaattta tcaaagtgta
    10681 tatgagtggt gagaaaaaag ctgctgttca aaatatagtt aagtaattta agaataaatt
    10741 caacgaaatg ttaagtattt taaattaaca ttttgtttag atagataaat tgaatattaa
    10801 aagaatatta aa
//
LOCUS       DAYAPA010000136        10745 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.140, whole genome shotgun sequence.
ACCESSION   DAYAPA010000136 DAYAPA010000000
VERSION     DAYAPA010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10745
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.140"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            146..610
                     /locus_tag="ACGI59_003254"
     CDS             146..610
                     /locus_tag="ACGI59_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HFG9741024.1"
                     /translation="MATMTDIIEKFIKDLMEEDNSIQIQRNELANLFSCAPSQINYVL
                     TTRFTIDRGYYIESKKGGGGYVQIEKIRKSKDGHIRELLNEKIGSQISYKKAKELLEG
                     LKESDLINERELKLILYAIDDKSLCMPIYELKEKVRSNILKNIIIGLFSIEE"
     gene            617..1111
                     /locus_tag="ACGI59_003255"
     CDS             617..1111
                     /locus_tag="ACGI59_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HFG9741025.1"
                     /translation="MLCQKCNKNKASVYYNKIVNGEKTEMYLCSECAKENTEMNFNLD
                     MPFSMMDIFSNLGFQPKKELEEKLVCPKCNTTYSEFKNNGRFGCSECYNAFSSQVNPM
                     LQNIHGHIEHTGKAPKKSFYKISVENEIKELKEDLDRAIKNEEYELAAQFRDKIKYLK
                     GSID"
     gene            1127..2152
                     /locus_tag="ACGI59_003256"
     CDS             1127..2152
                     /locus_tag="ACGI59_003256"
                     /EC_number="2.7.14.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0046314 - phosphocreatine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HFG9741026.1"
                     /translation="MKENIVMKSRVRLARNLNNYPFPNKLDKECAMEIIEKVKNAFIN
                     SNLEQKEEFDFYKIEDLDQSKKMLMVEEHIISPDLAENDKSAVIVKKDKTISIMINEE
                     DHIRIQTICDDLNLEYAYSVANEIDDLLESSLEYAFNTKLGYLTSCPTNTGTGMRASV
                     MMHLPALSQLGYMDELYKISSQIGIAIRGIYGERTEALGNIYQISNQLTLGRTESNII
                     ENVSGLTKDAISKEIKAREILQKKLGIKLEDKIFRSIGTLENSRVMSSAEAMSHLSNI
                     KMGIEMNYIDKLDLRAIEQLMIGIQPAHQSIMYKSDDVENRDINRATYIRETLEKLRG
                     GMNCELQ"
     gene            2139..4586
                     /locus_tag="ACGI59_003257"
     CDS             2139..4586
                     /locus_tag="ACGI59_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HFG9741027.1"
                     /translation="MNFNRFTQRAKKAIDLAFESAKSLGHNIVGSEHILLGLLREEEG
                     IAAKVLSKVGFTEAYLEGKIVDMEGKGEEISEDIVLSPRSKQILELSGMFANKLKTNY
                     IGTEHILLAIIQEGEGIANKILNYAGVNDRTLAQLTIDMMGISDKNQYKAENSYTGNQ
                     NQAESKVLDKYGRNLTLYAKQNKIDPVIGREKEIQRVIQILSRRTKNNPVLIGDPGVG
                     KTAIAEGLATNIALGNVPETLKNKTLYSLEMGSLLAGAKYRGEFEERIKEVVDEVVKN
                     GNIILFIDEMHTIIGAGSTGEGSIDASNILKPALARGEIQVIGATTIDEYRKHVEKDS
                     ALERRFQPVMVDEPTKEDSIKILEGLRDKYEAHHKVKITDDAIKAAVELSTRYISDRY
                     LPDKAIDLIDEAASKVRLKENTPPSEIKKLELEIENIDKEKEEAVRCQDFEKAAKIRD
                     EQGLLKKQLEDVRERWNKSSKHSDLVDGEVIAEVVGLWTGIPVNKILEEEADRLLRLE
                     EILHNKVIGQEQAVKSISKAIRRSRAGLKDPNRPIGSFLFLGPTGVGKTELSKALAEV
                     QFGDENQIIRIDMSEYMEKHAVSRMIGSPPGYVGHDEGGQLTEKVRRNPYSVILFDEI
                     EKAHPDVFNILLQILDDGRLTDSKGRTVDFKNTIVIMTSNVGASTIGRQKTLGFSIAK
                     GDEEEKSQYEKMKENIMGELKQRFRPEFLNRIDDIIVFHSLNENHISKIVLLMAAKLQ
                     ERLKEMDIKLEMSDEAVKLISKSGFDLEYGARPLKRALQKELEDELSEAILKGNVKKG
                     SNVVAKVKDEKIVFETK"
     gene            4766..6139
                     /gene="radA"
                     /locus_tag="ACGI59_003258"
     CDS             4766..6139
                     /gene="radA"
                     /locus_tag="ACGI59_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFG9741028.1"
                     /translation="MAKIKTKYVCQSCGYETAKWLGKCPECTKWNTFVEEIDQKSTKK
                     EVFIIDKSSSKPVSINSIESKEEERFTTDINELDRVLGGGIVKGSLVLVGGDPGIGKS
                     TLLIQVSSNVANLGKTVLYITGEESESQIKMRAKRLGINSEKLYIFAENNLSIIESYL
                     ESVNPELIILDSIQTVFSPEISSAPGTVSQIKEGTSKFMKISKKMGISTFIVGHVTKE
                     GSLAGPKLLEHMVDTVLYFEGERYNTYRLVRAVKNRFGSTNELGVFEMRDLGLVELDN
                     PSKILISEKPKDVAGSVIISTVEGTRPMLLELQALVSPTSFGIPKRTSTGVDYNRVGM
                     LLAVLEKRVGLQIQNQDVYINIVGGIKINEPSIDLGIAISVASSFRNIPIDEDIAVTG
                     EVGLTGEVRAVSFIEKRIAECKKLGFKKIVVPRSNYDVVKETKGIEIWPVDNLRQAIN
                     IVLGRNQ"
     gene            6142..7212
                     /gene="disA"
                     /locus_tag="ACGI59_003259"
     CDS             6142..7212
                     /gene="disA"
                     /locus_tag="ACGI59_003259"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning diadenylate cyclase DisA"
                     /protein_id="HFG9741029.1"
                     /translation="MENFLDNKNMLYALKMISPGTPLRLGLNNVLRAKTGGLIVIATN
                     EDVMKIVDGGFAINAEYSPSYLYELAKMDGAIVLSGDVKKILFANAQLIPDYFIETSE
                     TGTRHRTAERVAKQTGAIVIGISQRRNVITVYRGNEKYVVEDISKIFTKANQAIQTLE
                     KYKTVLDQAVTNLNALEFNDLVTIYDVALVMQKMEMVMRVTSIIEKYVIELGDEGTLV
                     SMQLEELMGTTRIDQKLIFKDYNKENTEIKELMKKVKNLNSEELIELVNMAKLLGYSG
                     FSESMDMPIKTRGYRILSKIHRLPTAIIENLVNYFENFQQILDASIEELDEVEGIGEI
                     RATYIKNGLIKMKQLVLLDRHI"
     gene            7301..8395
                     /locus_tag="ACGI59_003260"
     CDS             7301..8395
                     /locus_tag="ACGI59_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HFG9741030.1"
                     /translation="MIRKVTRILFILLGFTIGITTYLTLMKDFEILTFGKETYGYIAA
                     VVAGIIIAILGYLIEPWVVNKVKEIAKIVDKELSKYPQTDILLGSMGLIVGFVIAYLL
                     SGLVNRIPIVGGILSLLLYLFLGYLGMKVALKSKNDLFNIGKLGRLANPIKDKDKDKE
                     NKKEVKAIPPKVLDTSVIIDGRIADICKTGFIEGKLIIPAFVLEELRHIADSSDDLKR
                     VRGRRGLDILNIIQKELNIEVEISERDFDDIAEVDSKLLKLAQVLNGKVVTNDYNLNK
                     VAQFQGVEVLNINELANAIKPVAIPGEDMVVQVVKEGKEAQQGVAYLDDGTMIVVDGG
                     RKYMNETIKVLVTSVLQTPAGRMIFAKPKN"
     gene            8524..10701
                     /locus_tag="ACGI59_003261"
     CDS             8524..10701
                     /locus_tag="ACGI59_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9741031.1"
                     /translation="MRKKIISFLATFIIILTSASQYSFADDISTRGKVIFIDMNRTSM
                     SNMLRIKSLREELDNRGYIGLMNIRGDKGSDDRRSYASMGAGGRANVANEEDINFESS
                     SKDRNIVFESATGKSAKGINNLTINKSINENLNFGEYGSVLGSLGQSLSDNGLKASVL
                     GNSDIIENGQLIKNRNLCLTAMDEYGRIPNGNVDTINKKDLSMPYGISTDYDKLIVET
                     KEAYKNNDVIFVELGDTYRLDLYKPNLNEKTYESMKDNIEKNIDIYLKNIFSMVEEND
                     TVYIASAFPSDLDYKNKRRLSPIIKLNGEGKGILSSSTTRREGIVTNLDVGVEILDNF
                     NIKNQNMVGRKYELINRDDNKEFLMDEYQKIVSISSIRSTILNGFVSIVFLSWIVAMI
                     AILFRKHISKNYKETTFFILKELLKIGIVMPLSFMITPIMNFKTPLAISLGIIIITLT
                     IYLISKVLIKNDLKNMLFFTGLTIVIMVIDAGFGSYLMKSNVMSYDCIIGARYYGVGN
                     EYQGVAIGSAIFTFAILLTYKNIPKWSIIVFSLVILITSASPIMGANVGSAISECVAF
                     LLFILLIYNVKIDFKKIVLLGIAVVFVLGVFVAIDMILGSNSHLGMFVKEIYFNGPGE
                     IIQTFSRKIEMNLKLAQTSAWVNILLTGIGVILVLMINQIRYFKQLMDEYPIVFKGFI
                     ASIAGCLVTLLVNDSGIVSSATAFIYVIVPMITLSINLTALKE"
BASE COUNT         4483 a         1090 c         1999 g         3173 t
ORIGIN      
        1 ctcataatta atgaaagaat aaattcatat ctaaatttct ataaaaaatt taaatttata
       61 caatttaggg ctttaaaaat ttaaataata ggtgtaaaat aatagtcaaa gaaagtcaaa
      121 aataaaaagg tgtgtggtga tattaatggc aactatgaca gatataattg aaaaatttat
      181 taaagatttg atggaagaag ataatagcat acaaattcaa agaaatgaac tagcaaactt
      241 gtttagttgt gcaccatcac aaataaatta tgtcttaaca acaagattta caatagatag
      301 aggatattac attgaaagta aaaaaggtgg cggaggatat gttcaaatag aaaaaatacg
      361 aaaaagtaaa gatgggcata ttagagaact actaaatgaa aaaataggaa gtcagatatc
      421 ctataaaaaa gcaaaagaat tattagaagg tttaaaagag tcggatttga ttaacgaaag
      481 agaattaaaa ttaatattat atgcaataga tgataaatca ttgtgtatgc caatatatga
      541 attaaaagag aaggtaaggt ctaatatttt aaagaatata ataataggat tatttagtat
      601 agaggagtga taatttatgc tatgtcaaaa atgcaataag aataaagcat ctgtatatta
      661 taataagata gtcaatggag aaaaaacaga gatgtattta tgtagtgagt gtgcaaaaga
      721 gaatacagaa atgaatttta acttagatat gcctttttca atgatggata tattttctaa
      781 tttaggtttt caacctaaaa aagaacttga ggaaaagcta gtttgtccta aatgcaatac
      841 aacatatagt gagtttaaga ataatgggag atttggatgt agtgaatgtt ataatgcatt
      901 tagtagtcaa gtaaatccaa tgcttcaaaa tatacatggg catatagaac atacaggaaa
      961 agcacctaaa aaatcttttt ataagataag tgtagaaaat gagattaaag aactaaaaga
     1021 agatttagat agagctataa aaaatgaaga gtatgagtta gcagctcaat ttagagataa
     1081 aattaaatat ttaaaaggtt ctattgacta agattggagt gatattatga aagagaacat
     1141 agtcatgaaa tcaagagtta ggctagctag aaatttaaat aactatccat ttccaaataa
     1201 attagataaa gaatgtgcca tggaaataat agaaaaagta aaaaatgcgt ttattaattc
     1261 taacttagaa caaaaagaag aatttgattt ctataaaatt gaagatttag accaatctaa
     1321 aaaaatgctt atggttgaag aacatataat aagcccagat ttagcagaaa atgataaaag
     1381 tgcagttata gtaaaaaaag ataaaactat aagtataatg ataaatgaag aagaccatat
     1441 aaggatacaa actatatgtg atgatttaaa cttagaatat gcatatagtg tagcaaatga
     1501 aatagatgat ttgttagaat caagtttaga atatgccttt aacacaaagc taggttatct
     1561 aacatcttgt ccaactaata caggaacagg aatgagagct tctgtaatga tgcatttacc
     1621 agcattaagt caactaggct atatggatga actttataaa atttcatcac aaataggtat
     1681 agcaattaga gggatatatg gagaacgaac agaagcgttg ggaaatatat atcaaatatc
     1741 taatcaactt acacttggaa gaacagagtc taatattata gaaaatgtaa gtggacttac
     1801 aaaagatgca atttcaaaag aaataaaagc tagagaaata ttgcaaaaaa aattaggtat
     1861 aaaactagaa gataaaatat ttagatcgat agggacactt gaaaattcaa gagtaatgag
     1921 tagcgcagag gcaatgagcc atctatcaaa tataaaaatg ggaatagaaa tgaactatat
     1981 agataaacta gatttaaggg ctatagaaca acttatgata ggtatacaac cagcgcatca
     2041 gtcaataatg tataagagtg atgatgttga gaatagagat ataaatagag caacatatat
     2101 tagagaaact ttggaaaaat taagaggagg gatgaattgt gaacttcaat agatttacac
     2161 aaagagctaa aaaggcaatt gatttagcgt ttgaatctgc taaaagtcta ggacacaata
     2221 ttgtcggaag tgaacatata cttttaggtc ttttaagaga agaagaaggt atagctgcta
     2281 aagttctaag taaagttgga tttacagaag cttatttaga aggcaaaata gttgatatgg
     2341 aaggtaaggg agaagaaatc tcagaagata tagtattaag tccaaggagt aaacaaatac
     2401 ttgaattatc aggaatgttt gcaaataaat taaaaacaaa ttatattggg actgagcata
     2461 tattattagc aattattcaa gaaggtgaag gaatagctaa taagatttta aattatgctg
     2521 gagtaaatga taggacttta gcccaattaa caattgatat gatgggtatt agtgataaaa
     2581 atcaatacaa agcagagaat agttacacgg gcaaccagaa tcaagcagaa tctaaagtac
     2641 ttgataagta tggaagaaat cttacactat atgcaaaaca gaataaaata gaccctgtaa
     2701 taggtagaga aaaagaaata caaagagtaa tacaaatatt aagtagaaga actaaaaaca
     2761 atccagtgtt gataggagac ccaggtgtag ggaaaacagc catagctgaa ggtctggcta
     2821 caaatatagc cttaggaaat gttccagaaa cactaaaaaa caaaacttta tactctttag
     2881 agatgggttc attattggct ggagctaagt atagaggaga atttgaagaa agaattaaag
     2941 aagttgtaga tgaagttgtt aaaaatggaa acataatctt atttatagat gagatgcaca
     3001 caataatagg ggctgggtct actggagaag gctctataga tgcttcaaat atattaaagc
     3061 cagccttagc aagaggagaa atacaagtta taggtgcaac tacaattgat gaatatagaa
     3121 agcatgttga aaaagattct gctctagaaa gaagattcca accagttatg gtagatgaac
     3181 caactaaaga ggactcaata aaaatattag aaggattaag agataaatat gaagctcatc
     3241 ataaagttaa aataactgat gatgctataa aggcagctgt agaattatca actagatata
     3301 tatcagacag atatctgcca gataaagcta tagacttgat tgatgaagct gcatcaaaag
     3361 taagattaaa agaaaataca cctccttcag aaataaaaaa attagaacta gaaatagaaa
     3421 atatagataa agaaaaagaa gaagcagtaa gatgtcaaga ctttgagaaa gcagccaaaa
     3481 taagagatga acaaggtcta cttaaaaaac aattagaaga tgttagagaa agatggaaca
     3541 agtcatctaa acattcagat ttagttgatg gagaagttat tgctgaagta gtgggcttat
     3601 ggacaggaat accagttaat aaaattcttg aagaagaggc tgatagactt ttgagacttg
     3661 aggaaatact gcacaataaa gttataggtc aagaacaagc agtaaaatct atttcaaagg
     3721 caatcagaag gtcaagagca ggtcttaaag accctaatag accaattggt tcatttttat
     3781 ttttaggtcc aacaggagta ggtaaaacag aattatctaa ggcattagca gaagtgcaat
     3841 ttggagatga aaatcaaata attagaatag atatgtctga atatatggaa aaacatgctg
     3901 tgtcaagaat gataggttca cctccaggtt atgtaggtca tgatgaaggt ggtcaattaa
     3961 ctgaaaaagt aagaagaaat ccatattcag ttatcttatt tgatgagata gaaaaagctc
     4021 atccagatgt atttaatatt ctgttacaaa ttctagatga tggtagacta actgattcaa
     4081 aaggaagaac tgtagatttt aagaatacta ttgtaataat gacatcaaat gttggagcat
     4141 ctacaattgg tagacaaaaa actttaggat ttagtatagc taaaggagac gaagaagaaa
     4201 aatctcaata tgaaaaaatg aaagaaaata taatgggtga attaaaacaa agattcagac
     4261 cagagttttt aaacagaata gatgatataa ttgttttcca ctcactaaat gaaaatcata
     4321 tatcaaaaat agtactttta atggcagcga aactacaaga gagattaaaa gaaatggata
     4381 taaaattaga aatgagtgat gaggctgtta aattaatttc taaatctgga tttgacttag
     4441 aatatggagc aagacctctt aaaagagctt tacaaaaaga gttggaagat gagctatctg
     4501 aagcaatctt aaaaggtaat gtgaaaaagg gaagtaatgt agtagcaaaa gtcaaagatg
     4561 aaaaaatagt ttttgaaact aaataattta atagagttgc attttaaaat attgcaaata
     4621 taagttttat aatctaaaca aaattttaag ggcaatgaat ctataataat tcattgtcct
     4681 taattatttt atttaatctt tacatattgt ctttcagata tgctaaaatt atagtataga
     4741 ataaaaaatt aaggatgata aaagtatggc aaaaataaaa acaaaatatg tatgtcaatc
     4801 ttgcggatat gaaacagcta aatggcttgg taaatgtcca gaatgtacaa aatggaatac
     4861 atttgtagaa gaaatagacc aaaaaagcac caaaaaagag gtctttataa tagataaatc
     4921 ttcatctaaa ccagtcagta taaactcaat agagagtaaa gaagaagagc ggtttacaac
     4981 agatataaat gaattagata gagtgcttgg aggaggtata gttaaaggct ctttagtgct
     5041 tgttggtggt gaccctggta taggtaaatc aacactttta atccaagtat ctagtaatgt
     5101 tgccaattta ggaaaaactg ttttatatat aactggagaa gaatcagaat ctcaaataaa
     5161 aatgagagct aaaagattag gtattaactc agaaaaatta tatatatttg ctgaaaataa
     5221 tttaagtata attgaatctt atttagaaag tgtaaatcca gaattaataa ttctagactc
     5281 aattcaaact gtcttcagtc cagaaatatc ctcggcacca ggaactgtca gccaaattaa
     5341 agaaggtact tcaaaattca tgaaaatttc taaaaaaatg ggaatttcaa cgttcatagt
     5401 tgggcatgtt acaaaagaag gttcactagc aggaccaaaa ttattagagc atatggttga
     5461 tactgttcta tattttgaag gagaaagata taatacttat agattagtaa gagctgttaa
     5521 aaatcgattt ggttcaacta atgaacttgg agtatttgaa atgagggatt taggattagt
     5581 agaacttgat aatccatcta aaattttaat ctctgaaaaa ccaaaagatg tagcaggttc
     5641 agtaataata tctactgtag aaggtacgag gccaatgtta cttgaattgc aagcattagt
     5701 ttctcctaca agttttggta taccaaaaag aacatctaca ggagtcgatt acaatagagt
     5761 aggtatgtta ttagcagtat tagaaaaacg tgttggtttg caaatacaaa atcaagatgt
     5821 ctatataaac atagttggag gaataaagat aaatgaaccc tcaattgatt tagggatagc
     5881 catttctgta gcatctagtt ttagaaatat accgatagat gaagatatag ctgttacagg
     5941 agaagtggga cttacaggag aagttagagc agtaagcttt atagaaaaga gaatagctga
     6001 gtgtaaaaaa cttggattta aaaagatagt agttcctaga agtaattatg atgttgtaaa
     6061 agagaccaaa ggcatagaaa tatggccagt agataactta agacaagcta taaatatagt
     6121 acttgggagg aatcaataaa tatggagaat tttctagata ataaaaatat gctatatgca
     6181 ttaaaaatga tatctcctgg aactccactt agattaggtc taaacaatgt actaagagct
     6241 aagactggtg gattaattgt aattgcaaca aacgaagatg taatgaaaat agtagatgga
     6301 ggatttgcta taaatgcaga atattcacca tcatatctat atgaattagc taaaatggat
     6361 ggagctatag ttttaagtgg tgatgtaaag aaaatattat ttgctaatgc acaacttata
     6421 cctgactatt ttatagaaac atcagagaca ggaacaagac atagaacagc agaaagagta
     6481 gcaaaacaaa ctggtgctat agtcatagga atttcacaaa gaagaaatgt tataacagtt
     6541 tatagaggaa atgagaagta tgtagtcgaa gatatatcta agatatttac taaggcaaat
     6601 caggctatac aaactctgga aaaatataag acagtattgg accaagctgt aacaaattta
     6661 aatgccttag agtttaatga tttggtaact atttatgatg ttgcattagt catgcaaaag
     6721 atggaaatgg taatgagagt tacaagtata attgaaaaat atgtgataga attgggtgat
     6781 gaaggaactt tagtaagtat gcaattagaa gaattaatgg gtacaaccag aatagaccag
     6841 aaattaatat tcaaagatta taataaagaa aacacagaaa taaaagaact tatgaaaaag
     6901 gtcaaaaatt taaattcaga agaactaata gaattggtca atatggcaaa actattaggg
     6961 tatagtggtt tttcagaaag tatggatatg cctataaaaa caagaggtta taggattctt
     7021 agcaaaatac atagactacc aacagcaata atagaaaact tagtaaatta ttttgaaaac
     7081 tttcaacaaa ttttagatgc atctattgaa gaattagatg aggttgaagg aataggtgaa
     7141 ataagagcaa catatataaa aaatggactc ataaaaatga aacaattagt cttattagat
     7201 agacacatat gatgaatttt aaatgaattt tactactttt ttcttgaata taatatacac
     7261 tttggtctat aatagataag tataactagg gggtgaagca ttgataagga aagtaacaag
     7321 aatattgttt attttacttg gatttacaat aggtataaca acctatttaa cattaatgaa
     7381 agattttgaa atattaacat ttggaaaaga aacatatggt tatatcgcag ctgtagtagc
     7441 aggtataata atagccattt taggatatct tatagagcca tgggttgtaa ataaagtaaa
     7501 agaaattgct aaaatagtgg ataaagaatt gtcaaaatat cctcagactg atatattgtt
     7561 agggtctatg ggtcttatag taggatttgt tattgcgtat ttattaagtg gattagtaaa
     7621 tagaatacca atagtaggtg gaatactatc cttactatta tatttattcc taggatatct
     7681 tggaatgaaa gttgcgctta aaagtaaaaa tgatttattt aatattggaa aactaggaag
     7741 attagcaaat cctattaagg ataaagataa ggataaagaa aataaaaaag aagtaaaagc
     7801 cattcctcca aaagtattag atacaagtgt aataattgat ggaagaatag ctgatatttg
     7861 taagactggg tttatagaag gaaaacttat tattcctgct tttgttttgg aggaactaag
     7921 acatattgca gattcttcag atgatttaaa aagagtaaga ggtagaagag gtctggatat
     7981 attaaatata attcagaaag aattaaatat agaagttgaa ataagtgaga gagactttga
     8041 tgatatagca gaagttgata gtaaattatt aaagcttgct caggttttaa atggaaaagt
     8101 tgttacaaat gattataact taaataaagt tgctcaattc caaggtgttg aagtattaaa
     8161 tataaatgag ttagctaatg ctataaaacc agttgctata ccaggtgaag atatggttgt
     8221 tcaagtggtt aaagaaggaa aagaggctca acaaggtgtt gcatatctag atgatggaac
     8281 tatgatagtt gttgatggag gaagaaaata tatgaatgag actataaaag tactcgttac
     8341 ttctgttctt caaactcctg caggtagaat gatatttgca aaacctaaaa attaatatgt
     8401 tcaaagaaac ctataatttg ggctgtttat tttaaataga tggtccaaat ttatttattt
     8461 ataacgataa tgtttaatgt attcttaaag atggggattt aaatcagaaa gcagggagaa
     8521 tttatgagaa aaaagattat atctttctta gcaacattta tcataatatt aacatcagca
     8581 agtcagtata gttttgcaga tgatatatca acacgtggaa aagtaatatt tatagatatg
     8641 aatagaacta gtatgagtaa tatgttaaga ataaaatcat taagagaaga actagacaat
     8701 agagggtata ttggcttgat gaatataaga ggcgataaag gaagtgatga tagaaggtca
     8761 tatgctagta tgggtgctgg tggtagagct aatgtggcta atgaagaaga tattaatttt
     8821 gaaagctcga gtaaggatag aaatatagtt tttgaatcag ctacaggaaa aagtgcaaaa
     8881 ggtataaata atttaactat aaataagtct ataaatgaaa atttaaactt tggagagtat
     8941 ggttctgtat tagggtcatt aggacaaagt ttatctgata atggattaaa agcatctgta
     9001 ttgggtaatt cagatataat tgaaaatggt caacttataa aaaatagaaa cttatgtcta
     9061 actgcaatgg atgaatatgg aagaatacct aatggaaatg tagatactat aaataaaaaa
     9121 gacttatcta tgccttatgg aatatcaact gattatgata aattgattgt agagactaaa
     9181 gaagcttaca aaaataatga tgtgattttt gtggagcttg gagacacata tagattagat
     9241 ttatataaac caaatttaaa tgaaaaaaca tatgagagta tgaaagataa catagaaaaa
     9301 aatatagata tatatttaaa aaatatattt tctatggttg aagaaaatga cactgtctac
     9361 atagcaagtg catttccaag tgatttagat tataaaaata aaagaagact ttcgccaata
     9421 ataaaattaa atggagaagg gaaaggtata ttatcatctt caactactag aagagaaggt
     9481 attgtaacaa atttagatgt tggggttgaa atattagaca actttaatat aaaaaatcaa
     9541 aatatggttg gaagaaaata tgaacttatt aacagagatg ataataaaga atttttgatg
     9601 gatgaatatc aaaagatagt atctatttca agcataaggt ctacaatact gaatggtttt
     9661 gtaagtatag ttttcttgtc atggatagtt gctatgattg caattttatt tagaaagcat
     9721 atatcaaaaa attataaaga aacaacattt tttatattaa aggaactttt aaaaatagga
     9781 atagtaatgc cactgtcatt tatgataaca ccaataatga atttcaaaac accattagca
     9841 atatctttag gaataattat aataacactt actatatatc ttataagtaa agttttaata
     9901 aagaacgatt taaaaaacat gctgtttttt actggattaa ccatagtaat tatggttatt
     9961 gatgctgggt ttggctcata tttaatgaaa agcaatgtta tgagttatga ttgtataatt
    10021 ggagctagat attatggagt aggaaatgag tatcaaggtg tggcaatcgg tagtgcaata
    10081 tttacatttg caattttact tacatataaa aatataccaa aatggtctat aatagttttt
    10141 tctttggtta tcttaattac aagtgcatct ccaattatgg gagcaaatgt tggaagtgct
    10201 atttctgaat gtgttgcatt tttactattt atactattga tatataatgt aaagatagat
    10261 ttcaaaaaaa tcgtattatt aggtatagca gtagtatttg tattaggagt ttttgtagca
    10321 atagatatga ttttgggaag taattcacat ttaggcatgt ttgtaaaaga aatttacttt
    10381 aatggaccag gagaaataat acaaacattt agtagaaaga tagagatgaa tttaaagcta
    10441 gctcaaacca gtgcatgggt aaatatactg ttaactggaa taggtgtaat tttagttctt
    10501 atgattaatc agattagata ctttaagcaa ctgatggatg agtatccaat tgtatttaaa
    10561 ggatttatag catcaatagc aggatgttta gtaactcttt tagtgaatga ctctgggatt
    10621 gtgtcatcag ctacagcctt tatttatgta attgtaccta tgataacatt aagtataaat
    10681 cttacagcat taaaagaata agtactgtat gaataatatg taaatattaa tacattaatt
    10741 taact
//
LOCUS       DAYAPA010000137        10642 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.141, whole genome shotgun sequence.
ACCESSION   DAYAPA010000137 DAYAPA010000000
VERSION     DAYAPA010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10642
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.141"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            <1..321
                     /locus_tag="ACGI59_003262"
     CDS             <1..321
                     /locus_tag="ACGI59_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HFG9741032.1"
                     /translation="VEKADCYTGNTIESERFFPGWLYDEEDEFVQAAYKGLKEAGIDS
                     EITQYSFCTNGSHYAGEAGIKTIGFGPSKENLAHTIDEYIEQEQLFIGTEGYYGILKS
                     VYGK"
     gene            454..1824
                     /gene="hydA"
                     /locus_tag="ACGI59_003263"
     CDS             454..1824
                     /gene="hydA"
                     /locus_tag="ACGI59_003263"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HFG9741033.1"
                     /translation="MGTILRGGTIITSDKTYISDLRIEDEKIVEIGNNLEINGDKVID
                     ATGKIVIPGGIDTHTHFDMNAGSITTADNFETGTRAAIAGGTTTILDFAEANEGENLL
                     QGVEAYHKKASGNCYCDYGFHMTITCLNKDTFNHMEKLISDGIVSFKMYMAYDGMKVD
                     DGTIYRVLKKARDLGCIVGFHCENGDLLDVLIEENIQNGNLEPKYHPLTRPNIVEKES
                     VSRLADITKLSNSKSYVVHLSCRESLETVKIAREKNIDMIVETCPQYLLLEDNLYNKD
                     KFEGAKYVMSPPLRKKEDINYLWEGLSSGDIQTVGTDHCSFNFKGQKDLGIDDFSKIP
                     NGAPGVEHRLALLYTYGVLENRISVNKFVEVTSTNAAKIFGMYPKKGEIAVGSDADIV
                     ILNINKEETISYKTQKQNVDYTPYEGFKVKCKVEDVFLRGNHVVQSCNVKEHPTGQYI
                     KRKITK"
     gene            1842..3248
                     /locus_tag="ACGI59_003264"
     CDS             1842..3248
                     /locus_tag="ACGI59_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFG9741034.1"
                     /translation="MMDILIKNAIIVTVNKEREVIFDGALVVKDNKIADIGNSKEIES
                     KYTDVKKIIDAKGKVLFPGFINTHNHLFQTLLKGLGDDMVLKDWLETMTFPAANYLEP
                     KDTYDAAMLGCIEGLRSGITTMVDYMYPHSKPGLCDGIIDAYKELGIRGILGRGCMNT
                     GAQFGVHPGIMQDVETVEKDVRRLFEKHHNTENGRIKIGVAPAAIWSNSQEMLEMLWR
                     VVKEYDDALFTVHISETPFDREAAKELHGQYDIDVLEKLGILGPNVLMVHCVYLTEKD
                     MELTKKYDMKVSHNTASNMYLSSGVAPVPEMLKKGITVSLGVDGAASNNSQDMLELMK
                     LTALQHKVNKCDPLAMSAEKVLELATIDGARAIGMEDEIGSLEIGKKADLLIFNPMLS
                     PKAIPMHNPVSTLVYSSSMKNIESVIVDGNIIMEDSKILTANEEKALKDAQDTAERLC
                     IRGTIKNRMEGHKWNSLY"
     gene            3320..3766
                     /locus_tag="ACGI59_003265"
     CDS             3320..3766
                     /locus_tag="ACGI59_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HFG9741035.1"
                     /translation="MIIKCTINNKEKELSIRPDDFLADTLRENGYVSVKKGCDTGSCG
                     LCTVMVNDKAVLSCNYLTARVEGQHITTLEGVLDEAKKFGDFLADEGADQCGFCAPGF
                     IMMVLAMKRELQNPTEEEVIDYVNGNLCRCTGYKGQLRAIHKYLNT"
     gene            3805..4599
                     /locus_tag="ACGI59_003266"
     CDS             3805..4599
                     /locus_tag="ACGI59_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HFG9741036.1"
                     /translation="MITIKEYVVPKSLDEAYELLISRKNNIILGGCGFLKLGSKNIGS
                     AIDLKDLALDYINETDDSILIGADTSLRTLELNKVIKNYCNGVISNAVSNIVGVQFRS
                     GARVGASVFAKYGFSDLIPSLLVVDAKVKLYKKGVMNLADFLENELEKDILIEVILPK
                     KDAIGVFDSIRKCTGDFAVLNGAMLKEGSIYKIAIGARPRRAKIAYKASEILSLENDI
                     EKAGEVSSEELSFGSNIRGSKEYRKDMTKALVVRMYNSIGGECDGK"
     gene            4589..6880
                     /locus_tag="ACGI59_003267"
     CDS             4589..6880
                     /locus_tag="ACGI59_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HFG9741037.1"
                     /translation="MVNKEVRKIDSEAIVTGKPIYTEDLIFHKDVLTIKLLRSPHAFA
                     KIKNIDIKNALKVKGVVNIFTYKDVPNYRYSMVGESYPEASPHDQLILEDVVRYVGDE
                     VAIIAAEDERAAIKAMKLIKVEYEVMTPILDARESEGNKILIHEEDDLFCPFDFGLDA
                     TKNIVSREKMGKGDVEKEFKESDVIVEHSYMTQSQAHCMMETHRAYTYIDTNGRLVVT
                     SANQSAYHMRRQVSKALGIPLSKVRVIKPRIGGGFGGKNIAVTEIYASFVTWMTKRPA
                     KLIYTREETFAMTNTRHQMFFDIKIGADKEGNIKAIDMKALNNTGAYGDNGPSVCSES
                     GHNVLPTYNNVPAIKFDGKTVYTNLVPGGALRGYGATQGTFALDCAIDELAHKLNMDP
                     TEIKLKNIIKKQTEGGIMNFPIRSCALDECIIRGKKLIGWDEKYPVRDIGNNKVRAVG
                     MSVGTHGSGIANIDMAIVNMRMDEDGTYKLFSGSSDLGTGSDTILAQIAAKALNTSID
                     RISVYTGDTDMCPYDTGAYASCTTYVTGNATIKSAESLKQKILKVAQKKLDLPIDDLI
                     LYEDRVCYSEDVEKFVLLSELGKESVGGGNQEVLYSSESYGIQESAKPFLAGFAEIEL
                     DKTTGEFKVINYACSTDCGTVMNPALARIQVEGGVTQGIGLAMYEDPRHGSDGKLQTS
                     NLLQYKVPTKKDIGNIIVDFVDNYEPTGPYGAKSLGEIVIHTPAPAIANAIYNATGVR
                     IRELPITSEKVYMAMKKLNQGGK"
     gene            6881..8194
                     /locus_tag="ACGI59_003268"
     CDS             6881..8194
                     /locus_tag="ACGI59_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFG9741038.1"
                     /translation="MIAIKSKYLISSADEIYTNSAVVVENDIIKDILPNEEVEIKYKN
                     IEEIIDKSDAIIMPGFVNGHMHQYGVLSRGIPANVHFTDFEGFLNDYWWPFIENRIGL
                     KEVKATTKASAIELIESGVVAFCDTLEAPNTEEGTLIEQGKILEEIGMKAILSLESCE
                     RISFENGLRCLDENSNLIKWSRENSKLINGIMCTHTSFTCSDRFIKKAKEDAKKLNAP
                     MQFHLCESIYEPNYAEKHFGKKAVDYYNDLDILDETVLASQCVKVNDEEIDILKEKGV
                     KVVHMPLSNCEVGGGFSPVPKMIKKGIKVALGTDGYINDFFEVMRGAFLMHKSVEEDA
                     SVMPANLVFRMATEHGAYVLGLQNSGKIAVGNKADIIVMEDEFKTPVTLDNIFDQIVV
                     QGKKEFISNVYIDGRHILKEKQLVDLDKKAIVKEMKEVACEFWKF"
     gene            8230..9315
                     /locus_tag="ACGI59_003269"
     CDS             8230..9315
                     /locus_tag="ACGI59_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HFG9741039.1"
                     /translation="MYNFLGKELKSPLIIGSGPLTYSAAGCKILSDAGAGAVVTKTIR
                     KERAINPAPHMVRNTANALLNNEKWTDFEPEQWIDFEIPQMKRDGTVCIASIGHTIEE
                     SSELVEKVANAGADFIELVSYDYRDLIPMLKDAKERVNIPVIVKLPPMIDEIGDFAKK
                     LEEAGADAITACDSVGPAFRIDIETGQPLLGGNGIGYLSGETIKPITLQRIYEIKKQV
                     NIPIIGLGGCVSGDDALEMIMAGADFVGICSVVILKGAQVISKIHDDLKSNLNRLGYN
                     TIENACGIVHNHMGDVNERLSFEFTYDEEKCTKCKMCERVCAYQARKLNDKMELNEEE
                     CRYCGLCISVCKPKALGRKISCSELNV"
     gene            9551..10594
                     /locus_tag="ACGI59_003270"
     CDS             9551..10594
                     /locus_tag="ACGI59_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9741040.1"
                     /translation="MQVQEKILELLYTINEDVVSIKNDMSEVKQEINVLKEDMSEVKQ
                     EMTVMKEDMSEVKQEINVLKEDMSEMKQEMTVMKEDMSEVKQEINVLKGDMSEVKQEM
                     IVMKEDMSEVRQEINIMKEDMSEVRQEINTMKEDMSEVRQEMTVMKEDMSEVKQEINV
                     LKEDMSEVRQEINIMKEDMSEVRQEMTVMKEDTSEVKQEINVMKKDMSEVRQEITVMK
                     EDVSEVKSEVNFMQNKINNINEDMNGIKDEVSIANEKLYGIEIKIDSLECEDKSMKEI
                     QIEQNNILGSLLHNSEINKATHDNIEHNIAYIKGDTNSIKEDIAEIRRDLNLVELATS
                     KNWSDIVKLKSVK"
BASE COUNT         4285 a         1117 c         2169 g         3071 t
ORIGIN      
        1 gttgaaaaag ctgattgcta tacaggaaat actatagaaa gtgaaagatt cttcccaggt
       61 tggttatatg acgaagaaga tgaatttgtt caagcagcgt ataagggatt aaaagaagct
      121 ggaatagatt cagaaataac tcaatactca ttctgtacga atggaagtca ctatgcaggt
      181 gaagctggaa taaaaactat aggatttggt ccttctaagg aaaatcttgc tcacacaata
      241 gatgagtata tagagcaaga acagttattt ataggaacag aaggttacta tggaatatta
      301 aaatctgtat acggaaaata atatttagtt cacaataaat tatttaaata tagtctagag
      361 gtgctgaata tagcaccttt agaaaataca attttaaata aagttttaca atacttagaa
      421 aatactttaa agcttaaaag ttaggaggta tatatgggaa caatactaag aggtggaact
      481 ataattacct ctgataagac ttatataagt gatttaagga tagaagatga aaagatagtt
      541 gaaataggaa ataacttaga aataaatggt gataaggtta ttgatgctac aggtaaaatt
      601 gtaattcctg gtggaataga cacacatact cattttgata tgaatgcagg ttctattaca
      661 actgcagata attttgaaac aggaacaaga gcagctatag ctggaggaac tactacaata
      721 ctagattttg cagaagccaa tgagggggaa aatctactgc aaggtgtaga agcatatcat
      781 aaaaaagcaa gtggaaattg ttattgtgat tatggttttc atatgacaat tacatgttta
      841 aataaagata catttaatca tatggaaaaa cttatttcag atgggatagt atcttttaag
      901 atgtatatgg catatgatgg catgaaagta gatgatggaa caatatacag ggttttaaag
      961 aaagcaaggg atttaggttg tatagttgga tttcattgtg aaaatgggga tttactagat
     1021 gtattgatag aagaaaatat acaaaatggt aatttggagc caaaatatca tccacttaca
     1081 agacctaaca tagttgagaa agagtctgtt tcaagacttg ctgatataac taaattatct
     1141 aattcaaaat cctatgtagt ccacttaagt tgtagggagt cacttgaaac tgtaaaaata
     1201 gctagagaaa aaaatataga tatgatagta gaaacatgcc cacagtatct attattagaa
     1261 gacaatttat ataataagga taaatttgag ggtgctaagt atgtaatgtc tcctccttta
     1321 agaaaaaaag aagacataaa ttacctttgg gaaggattgt ctagtggaga tattcaaaca
     1381 gttggcacag accactgttc atttaatttt aaggggcaaa aagacttggg aattgatgat
     1441 ttcagtaaga taccaaatgg agcacctggt gttgaacata ggttagcact tttatataca
     1501 tatggggtct tagaaaatag aataagtgta aataagtttg tggaagttac ttctacgaat
     1561 gctgcaaaaa tatttggaat gtatcctaaa aaaggtgaga tagctgtagg aagtgatgca
     1621 gatatagtca ttttaaatat caataaagaa gaaacaatta gttataaaac tcaaaaacaa
     1681 aatgtagatt atactccata tgaaggattt aaggttaaat gtaaagttga agatgtattt
     1741 ttgagaggaa atcatgtagt acaaagttgt aatgttaaag aacatccaac aggtcaatat
     1801 attaaaagaa aaattactaa ataatgcggg gggaaataag aatgatggat atacttatta
     1861 agaatgccat aatagtaact gtgaataaag agagagaagt tatttttgat ggggcattag
     1921 tagtaaaaga taacaagatt gctgatattg gaaattctaa agagatagaa agtaaatata
     1981 ctgatgttaa aaaaattata gatgctaaag gtaaagtttt attcccaggt tttataaata
     2041 cacataatca tttattccaa actttattaa aaggattggg agatgatatg gtacttaaag
     2101 actggttaga aactatgaca tttccagcag ctaactattt agagcccaaa gatacttatg
     2161 atgctgcaat gcttggatgt attgaaggac ttagaagtgg tataacaaca atggttgact
     2221 acatgtatcc tcatagtaag ccaggtcttt gtgatggaat aatagatgct tataaagaat
     2281 taggaataag aggaatactt ggaagaggat gtatgaatac aggagctcaa tttggagttc
     2341 atcctggaat aatgcaagat gtagaaacgg ttgaaaaaga tgtaagaaga ttgtttgaaa
     2401 aacaccataa tacagaaaat ggaagaatca aaataggagt agctcctgca gcaatatggt
     2461 caaattctca agaaatgctt gaaatgctat ggagagtagt aaaagaatat gatgatgctt
     2521 tatttacagt acatatatca gagactcctt ttgatagaga agcagcaaaa gaattgcatg
     2581 ggcaatatga catagatgtt ttagaaaaat taggaatact tggaccaaat gtgcttatgg
     2641 tacattgtgt gtatctaact gaaaaagata tggaacttac taaaaaatac gatatgaaag
     2701 tgtctcataa tacagcaagt aatatgtact tatcttcagg tgttgcacca gtgccagaaa
     2761 tgcttaaaaa aggaataaca gttagtttag gagtagatgg agccgctagt aataactctc
     2821 aagatatgct tgaacttatg aaattaacag cacttcaaca taaagttaat aagtgtgacc
     2881 cattagcaat gtcggcagaa aaagttcttg agttggcaac tatagatgga gcaagagcta
     2941 ttggaatgga agatgagata ggttcattgg agataggtaa aaaagcagac ttactaatat
     3001 ttaatccaat gctttcacca aaagcgatac ctatgcataa tccggtatct acattagtat
     3061 attcttctag tatgaaaaat atagagagtg ttatagtaga tggaaatata ataatggaag
     3121 attcaaaaat attgacagct aatgaagaga aagcattaaa agatgctcaa gatacagctg
     3181 agagactttg tataagagga actataaaaa atagaatgga aggtcataaa tggaatagcc
     3241 tttattaata aatgtaattt attcataaaa aagtatagta aaactttagc ttaaaaatat
     3301 gagttttgag gtgatttatt tgattatcaa atgtaccata aataataaag aaaaagaatt
     3361 aagtataaga ccagatgatt ttttagcaga tacattaaga gaaaatggat atgtaagtgt
     3421 taaaaaaggg tgtgatacag gctcatgtgg gttatgtaca gtgatggtaa atgacaaggc
     3481 cgtgctttca tgtaattatc ttactgctag agtagaaggt cagcatatta caacacttga
     3541 gggtgtcttg gatgaagcaa agaagtttgg agattttctt gcagacgaag gggctgacca
     3601 gtgtggattt tgtgctcctg gatttataat gatggtcttg gcaatgaaaa gagagcttca
     3661 aaatccaaca gaagaagaag ttatagatta tgtaaatggt aacctttgta gatgtacagg
     3721 atataaaggt caattaagag caatacataa atatttaaac acttagactt ttaagtgaaa
     3781 attaagttga tatggggaga gagtatgata actataaagg aatatgtagt tccaaaaagt
     3841 ttagatgaag cttatgaact gctaatatca agaaaaaaca acataatact tggaggatgt
     3901 ggatttttaa aactaggtag taaaaatatt ggttcagcaa tagatttaaa agacttagct
     3961 ttagattata taaatgaaac tgatgatagc atattaattg gagcagatac aagtttaaga
     4021 actttagaat taaataaggt tattaaaaat tactgtaatg gggtaatttc aaacgctgtt
     4081 tctaatatag taggagttca atttagaagt ggagctagag taggagctag cgtatttgct
     4141 aagtatggat tttcagattt aataccttca ttactagtgg tagatgcaaa ggttaagtta
     4201 tataaaaaag gggttatgaa cttagctgat tttttagaaa atgagcttga aaaagatata
     4261 ttaatagaag ttatattacc taaaaaagat gctataggtg tatttgattc tataagaaaa
     4321 tgtacaggtg attttgcagt attaaatgga gcaatgttaa aagaaggaag tatttataaa
     4381 attgcgatag gcgctagacc aagaagagca aaaattgcct ataaagcaag tgaaatatta
     4441 agtttagaaa atgatataga aaaagctggt gaagtatcaa gtgaagaact ttcatttgga
     4501 agtaatataa gaggaagtaa agaatacaga aaagatatga caaaagcctt agtagtgaga
     4561 atgtataata gtattggagg tgagtgtgat ggtaaataaa gaggtaagaa aaatagatag
     4621 tgaagccatt gttactggaa aaccaatata tacagaagat ttaatttttc ataaagatgt
     4681 tttaacaatt aagcttctta gaagtccaca tgcttttgcg aagataaaaa acatagatat
     4741 aaaaaatgca ttaaaggtaa aaggtgtagt aaacatattc acttacaaag atgttccaaa
     4801 ttatagatat tcaatggttg gagagtcata tccagaagca tcacctcatg accaattaat
     4861 acttgaagac gtagttagat atgtaggaga tgaagttgct ataatagccg ctgaagatga
     4921 aagggctgct attaaagcta tgaaacttat aaaagttgaa tatgaagtaa tgactcctat
     4981 attggatgca agagaatcgg aaggaaataa aattttaatc catgaagaag atgatttgtt
     5041 ttgtccattt gattttggat tggatgctac aaaaaatatt gtatcgagag aaaaaatggg
     5101 taaaggggat gtagaaaaag agtttaaaga aagtgatgta atagtagaac attcttatat
     5161 gactcaatca caagcccatt gtatgatgga aactcataga gcttatacgt atatagatac
     5221 gaatggcaga ttagtagtaa cttctgcaaa ccaatcagct tatcatatga gaagacaggt
     5281 ttctaaagca ctgggaatac ctctttcaaa agtgagagta ataaaaccaa gaataggtgg
     5341 aggttttggt ggaaaaaaca tagctgtaac agagatatat gcatcatttg taacttggat
     5401 gacaaaaaga ccagcaaaac taatttatac tagagaagaa acttttgcaa tgactaatac
     5461 tagacatcag atgttttttg atataaaaat tggggcagat aaagaaggaa acataaaagc
     5521 tatagatatg aaagcattga acaatacagg cgcttatggc gataatggac cttcagtttg
     5581 ctctgaatca ggtcataatg tacttccaac atataataat gtacctgcaa taaaatttga
     5641 tgggaaaact gtatatacta atttagttcc aggaggagca ttaagaggat atggagcaac
     5701 acaaggtaca tttgcacttg attgtgcaat tgatgaatta gcgcataaac ttaatatgga
     5761 cccaacagaa ataaagttaa agaatattat aaaaaaacaa actgaaggcg gaataatgaa
     5821 ttttccaata agaagttgtg cacttgatga atgtataatt agaggtaaaa aacttattgg
     5881 atgggatgaa aagtaccctg ttagggatat tggaaacaat aaggtgaggg ctgttggaat
     5941 gtctgtggga actcatggtt ctggtatagc caacatagac atggcaatag taaatatgag
     6001 gatggatgaa gatggaactt ataagttgtt ttctgggtct tcagacttag gaactggttc
     6061 tgacacaata cttgcacaaa tagctgcaaa agcattgaat acatctattg atagaatttc
     6121 cgtttataca ggagatacag atatgtgtcc atatgatact ggtgcatacg cttcttgtac
     6181 tacttatgta actggaaatg ctacaataaa atctgctgaa agtttaaaac aaaagatatt
     6241 aaaagtagca cagaagaaac tcgatttacc aatagatgat ttaatactat atgaagacag
     6301 agtttgttat agtgaagatg tagagaagtt tgttttgtta agtgaattag gaaaagaatc
     6361 tgttggtggt ggaaatcaag aagttttata ttcatctgaa tcttatggta tacaagagag
     6421 tgctaaacca ttcttagcag gatttgctga aatagagtta gataaaacaa caggggagtt
     6481 taaagtaata aattatgctt gttctacaga ttgtggtaca gttatgaatc ctgcacttgc
     6541 tagaatacaa gttgaaggtg gtgtaactca aggtataggg cttgcaatgt atgaagaccc
     6601 tagacatggt tctgatggaa agcttcaaac ttctaatcta ttacaataca aagttccaac
     6661 aaaaaaagat ataggaaaca taattgttga ttttgtagat aattatgagc caacaggtcc
     6721 atatggagct aaatctttag gagaaatagt tattcataca cctgcaccag caatagcaaa
     6781 tgcaatatac aatgccacag gagttcgtat aagagaactt cctataactt ctgagaaagt
     6841 ttatatggcc atgaaaaaat taaatcaggg aggtaaataa atgattgcta ttaaaagtaa
     6901 atacttaata tcttctgctg atgaaatata tacaaattct gctgttgtag tagaaaatga
     6961 cattataaaa gatatattgc caaatgaaga agttgaaata aaatataaaa atattgaaga
     7021 aataatagat aaatcagatg ctataataat gccaggattc gtcaatggtc atatgcatca
     7081 atatggagtt ctttctaggg gaataccggc aaatgtacat tttactgact ttgaaggttt
     7141 tttaaatgac tattggtggc catttataga aaatagaatt ggattaaaag aagtgaaggc
     7201 aacaactaaa gcatcagcaa tagaacttat agaatctggt gtggtagctt tttgtgatac
     7261 cctagaagct ccaaatacag aagaaggaac tttaattgag caaggaaaaa tactagaaga
     7321 aataggcatg aaagcgattt tatccttaga aagttgtgaa agaataagct ttgaaaatgg
     7381 acttagatgt ttagatgaaa attctaattt aataaagtgg agtagagaaa atagtaaatt
     7441 aataaatgga ataatgtgta cacatacaag cttcacatgt tctgatagat ttattaagaa
     7501 agctaaagaa gatgcaaaaa aattaaatgc accaatgcaa tttcatttat gtgagagtat
     7561 atatgagcca aactatgctg aaaaacactt tggcaaaaaa gcagttgact actataatga
     7621 tttagatata ttggatgaaa ctgtattagc atctcagtgt gtaaaagtaa atgatgaaga
     7681 aatagatata ttaaaagaaa aaggcgtaaa agtggttcat atgcctctta gtaactgtga
     7741 agtaggtgga ggtttttctc cagtaccaaa aatgattaaa aaaggcataa aagtggcact
     7801 gggtactgat ggatatataa atgatttttt tgaagttatg agaggtgcat ttttaatgca
     7861 taaatcagtt gaagaagatg catcagtaat gccagcaaat ttagtattta gaatggcaac
     7921 agagcatggt gcttatgtat taggacttca aaattctggt aaaatagcag ttggtaataa
     7981 ggctgatata atagttatgg aagatgaatt taaaactcct gtgactcttg ataatatatt
     8041 tgaccagata gtagttcagg gtaaaaaaga atttatatca aatgtataca tagatggtag
     8101 acatatttta aaagaaaaac agttagttga tttagataaa aaagctattg ttaaagaaat
     8161 gaaagaagtt gcttgtgaat tttggaaatt ttaacgaatg taaatatata tgaaaagggg
     8221 aagcagataa tgtataattt tttaggtaaa gaattaaaat caccacttat aataggttca
     8281 ggaccattaa catatagtgc agctggatgc aagatattaa gtgatgcagg agcaggggca
     8341 gttgtgacta agactataag aaaagaaaga gctataaatc cagcaccaca tatggttaga
     8401 aatacagcta atgctctttt aaataatgaa aaatggacag attttgagcc agagcaatgg
     8461 atagattttg aaataccaca aatgaaaaga gatgggactg tctgtatagc ttcaataggt
     8521 catacaattg aagaaagtag cgaattagta gagaaagttg caaatgcagg agctgacttt
     8581 attgaattag tatcttatga ctatagagat ttgataccaa tgcttaaaga tgcaaaggaa
     8641 agagtaaata tacctgtaat agttaaactt cctcctatga tagatgagat aggcgatttt
     8701 gctaaaaaat tagaagaagc aggagcagat gcaataactg cttgtgactc tgtaggacca
     8761 gcatttagaa tagacataga aacagggcaa cctttattag gtggaaatgg aataggatat
     8821 ttaagtggag aaactatcaa gccaataact ttacagagaa tatatgaaat aaaaaaacaa
     8881 gttaatatac caataattgg cttaggtgga tgtgtaagtg gagatgatgc cttagaaatg
     8941 attatggcag gtgctgactt tgttggtata tgttcagtag taattttaaa aggtgcacaa
     9001 gtaatatcta aaatacatga tgatttaaaa tctaatctta atagattagg atataatact
     9061 atagaaaatg cttgtggtat agtacataat catatgggag atgttaatga aagacttagc
     9121 tttgagttta cttatgatga agaaaaatgt actaaatgta agatgtgcga gagagtttgt
     9181 gcatatcaag cgagaaaatt gaatgataag atggaattga atgaagaaga gtgtagatat
     9241 tgtggacttt gtatatcagt ttgtaaacca aaagcacttg gaagaaaaat ttcatgttca
     9301 gagctgaatg tatagattat tttataaaaa acaaagtttt aaataaaaat aagaaaggct
     9361 atctcaaaga gataagcctt ttttgttgtt gaccttattt ttgtttattt gtagttattt
     9421 tagatattaa ttagttttga agtaataaag tgaatcaatt ttgtttaatt aaaattttaa
     9481 taattctgat atatatgtta acattgtata gcatttgtgt aaaatgttat aataattaat
     9541 agggatggtg atgcaagtgc aagagaagat tttagagtta ttatatacta taaatgaaga
     9601 tgtagttagc ataaaaaatg atatgtcgga agtaaaacaa gaaataaatg tgttaaaaga
     9661 agatatgtcg gaagtaaaac aagaaatgac tgtaatgaaa gaagacatgt cggaagtaaa
     9721 acaggaaata aatgtattaa aagaagatat gtcggaaatg aaacaagaaa tgactgtaat
     9781 gaaagaagat atgtcggaag taaaacagga aataaatgta ttaaaaggag atatgtcaga
     9841 agtaaaacag gaaatgattg taatgaaaga agatatgtcc gaggtaagac aagagataaa
     9901 tataatgaaa gaggatatgt ccgaggtaag acaagagata aatacaatga aagaggatat
     9961 gtcggaagta agacaagaaa tgactgtaat gaaagaagat atgtcggaag taaaacagga
    10021 gataaatgta ttaaaagaag atatgtccga agtaagacaa gagataaata taatgaaaga
    10081 agatatgtcc gaagtaagac aagaaatgac cgtaatgaaa gaagatacat cagaggtaaa
    10141 acaagagata aatgtaatga aaaaagacat gtcagaagtg agacaagaaa taactgtaat
    10201 gaaagaagat gtttccgaag ttaaatcaga agtaaacttt atgcaaaata aaataaataa
    10261 cattaatgaa gatatgaatg gaattaagga cgaggtatcc atagcaaatg aaaaattata
    10321 tggaattgaa attaagattg atagtctaga atgtgaagat aaatcaatga aagagataca
    10381 gatagaacag aataatattt taggaagttt attacataat tctgaaataa ataaagctac
    10441 tcatgataat atagaacata acatagcata tataaaaggt gacactaatt ctataaaaga
    10501 ggatatagct gaaataagaa gagacttaaa tttagtagag ttagccacta gtaaaaattg
    10561 gagtgatatt gtcaaactaa aatcagtaaa gtagtatttt aattcactaa tttatcatcc
    10621 acaaactaag aattttatat aa
//
LOCUS       DAYAPA010000138        10481 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.142, whole genome shotgun sequence.
ACCESSION   DAYAPA010000138 DAYAPA010000000
VERSION     DAYAPA010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10481
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.142"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            235..1557
                     /locus_tag="ACGI59_003271"
     CDS             235..1557
                     /locus_tag="ACGI59_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HFG9741041.1"
                     /translation="MFDIDGRPPILKCLPLSMQHILAMIVGTVTVPIVVGGAVGVSPT
                     QITLLVQYALIIAGISTLIQLYPIGNIGSRLPIIFGVGFTYVPTLVAVGGNYGLASIL
                     GAQLIGGVATVLIGIFIKKIRKFFPNIVAGTVVFTIGLSLYPIAINYMAGGVNSPTYA
                     SMQNWAVAAITLVVVIGLSQFAKGYAKLAAIFLGIIVGYCVSLVLGIINFDPIREATW
                     FAIPKPLEFGMEFDMPVIISVVIVSIINAVQAIGDLSATTMGGMDRNITDKELSSGVI
                     GSGICTMIGALFGGLPPSSYSQNVGMVAMNKVISRFVLGIAGIFMLLSGFVPKFGALM
                     TTIPYSVLGGATISIFSMITMTGIKLIITEELSSRNITIVGLAIALGMGITSVPAAQE
                     AFPSFVKLVFGESPIVIATLVAFVLNLIIPNKSLEDEAKERAVMEETN"
     gene            1854..3215
                     /locus_tag="ACGI59_003272"
     CDS             1854..3215
                     /locus_tag="ACGI59_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HFG9741042.1"
                     /translation="MKTRNTSKYDVDGIPPLKESIPLALQHLLAMIVGNMVPAILIAN
                     VVGLNQGQATMLIQGSMLAAGLATFLQLYPIPLFKGFKLGSRLPVMMGMSYVFLGACL
                     SVAAKDGLAALFGAQIAAGVIVFFVGFGVKKIRHIFTPIVSGTIIACMGLGLFATAIK
                     NLAGGEGTQTFGSPINFTVGVIVAFVIIMINKYGKGLVKNSSILIGILVGYAISLVLG
                     LVDFSAVQGAAIVSLPTPAAFGLEFRPELIVMFTIIYIIGIADMMGACTIATVGAMDR
                     EVTDEELASVVLGNSITSIISSLFAALPTGVFSQNTVIVSMNKVTSRFVIALGALVLL
                     LAGISPALGAIMTTIPSCVVGGATLVVFSSIAMSGFSIMSMDGFTEENNLIAGVSIAT
                     SMGLTTAPQVLDQFPETIGTVLGGSSIVSGAIIAILLQTLFKLKSRKSAENVTSNLEE
                     NIG"
     gene            3288..4682
                     /locus_tag="ACGI59_003273"
     CDS             3288..4682
                     /locus_tag="ACGI59_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFG9741043.1"
                     /translation="MIDILIKNAIIVTVNKDRDVIFDGAIAIQGDRILDIGNTVDIEP
                     KYLDAKKVIDANGKVIFPGFINTHNHLFQVLLKGLGDDMALHEWLNTMMFPSAKFLTE
                     QDTYDAAMLGCMEGLKSGITTMVDYMHTHNRPGLTDGIVKAYKDLGIRGVVGRGCIDL
                     GIHKELIEDVETVEKDLRRVFEKYHNSENGRIKICVAPSSMWAISEEMGKMLWNIVKE
                     YDSYFTVHISETEFARTATKDIHGEIDIKLLEKWGIVGPEVVAVHCVCITDEDMEMLK
                     KYDIKVSHNVASNMYLASGVAPVPEMLKKGINVSLGLDGAASNNAQDMVELMKLTALQ
                     HKVNNCDPLAISAEKVLEMATIEGARTLRMEDEIGSLEIGKKADLVIFNSMLSPKSIP
                     LHNPVSTLVYSASMHNVESVLVDGNVILENGKVTTIESEEQVLLNAQHAAEELCKRAN
                     VTNRMEGHKWNSLY"
     gene            4744..5214
                     /locus_tag="ACGI59_003274"
     CDS             4744..5214
                     /locus_tag="ACGI59_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HFG9741044.1"
                     /translation="MEKIKLNINDYMYEIEVEKNETLLHVLREKLNLTGAKQGCNSGS
                     CGACKVLIDGVDMKSCKLLAKNCEGKVIYTIESFANGENLHPIQEAFIEVGAVQCGYC
                     TPGMIITTQALLNRNASPNEDEIREAFKENLCRCTGYVKIIEAVKLSAARLGGR"
     gene            5217..7433
                     /locus_tag="ACGI59_003275"
     CDS             5217..7433
                     /locus_tag="ACGI59_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HFG9741045.1"
                     /translation="MEKVIGKSYPLKDAALKVTGQMKYVADMKPQNVLYAKMLLSPVA
                     HAKIKSIDTSEAEKVVGVRAIATYLNTSQRKYNGSLRFYEHKIPENEVIFSDTVRFVG
                     DRVAAVAAETPEIAEKAVKLIKVEYEELPAVFTIQDAIKEDAICIHGDSNIVSQNNIN
                     GGDVEKGFKECDYIFEDEYSTPAIHHSAIETHSVIAHYDYNDKLTVHTPCQNTFGFRI
                     ILSQIFGMSLNKVRVIRPAIGGSFGGKLELTIEPVAAALSKMTRRPVKMVLTRKESMI
                     STRTRHGSFVKLKTGVNKDGEVIAQDIKIYTNTGAYASSALNVIGALSHKVFKVYKIP
                     NIKFTGMPVYTNTPIAGAMRGYGSPQIFMAQQAQFAKIAKEIGMDLVDFQNKNAVEPD
                     DVDIIFHGSLGNPRVLDCIEQGKKMFKWDEKKKLPKEEGKYLRGIGMAIGAHGNGVFG
                     AHRDVVTLTLKLNEDGTLTLLTGAHDMGNGVVTMQTMMVAEVLGITPDKVDTFETDTD
                     ACTFNLGDYASRGVFVEGGGAKKTAEKLKNLILEEAEKLLETSKEDLYLDNGCVISKL
                     DENVKASLSDVAVYSQSKSLRELTVVEDYSSPAGRTSYGVHFAEVLVDKETKDIKVVD
                     FVAVHDVGRVINPLNLEGQLEGGIHMGLGYALSEELAFDEQGRPKATDFKTYKLFRTV
                     DMPKCQVAFVDGYEKAGPFGGKSIGECAVVPVAPAVANAVYNATDVEIHSLPIKLK"
     gene            7516..8187
                     /locus_tag="ACGI59_003276"
     CDS             7516..8187
                     /locus_tag="ACGI59_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HFG9741046.1"
                     /translation="MAKILGICGSPRKGATEYAVLEALKEAETIPGIETEYWSVRGKK
                     ISPCVHCDACIRKETMCIIKDDIQELEQKILEADGIIVGSPVYDMNITAQLTAVFNRL
                     RPMYLVHPGKLQNKVGAAITTGGTRYGGQELTKLPIINFFLMHEMLVSGGLGGCYIGG
                     TIWSKDGKAKGAQEDETGMDTVKRLGKGAAEAVMVSKFGLEKWNVVKEELDVKKDEKS
                     PLRDH"
     gene            8550..9764
                     /gene="dpaL"
                     /locus_tag="ACGI59_003277"
     CDS             8550..9764
                     /gene="dpaL"
                     /locus_tag="ACGI59_003277"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HFG9741047.1"
                     /translation="MLKEIKWKVNNLPKGDKENCIKFLNEEEITKVRNFHKSFPQYKE
                     TPLANLEGLAKKLGVAGVYVKDESYRFGLNAFKVLGGSYSMGRYLAQRLDTDISELGY
                     DKLTSKEIKEKLGEITFFTATDGNHGRGVAWTANKLGQKSVVLMPKGSSEFRLNKIKG
                     EGADASITDLNYDDAVRLANDYAEADDHGVMVQDTAWDGYEEIPAWIMQGYGTMAQEA
                     IEQLKEYGVDRPTHVFVQAGVGSLAGAVQGYVASIYDECPITVVVEADEADCYYKSAE
                     AGDGKPRFVGGDMPTIMAGLACGEPNTIGFEVLKNHAAAFVSAPDWVSAKGMRTLGNP
                     LNGDEKVISGESGAVTTGLLVAAMEREDLADLRKDLKLDENSRILLISTEGDTDPDKY
                     RSIVWDGEYPSI"
     gene            9891..>10481
                     /locus_tag="ACGI59_003278"
     CDS             9891..>10481
                     /locus_tag="ACGI59_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HFG9741048.1"
                     /translation="MLNEQRKQEVTEICQKLIQNPSSSGNEEGVVKAIEESFEKLGFD
                     SWSRDRYGNIVGCIKGNKPGKKILFDGHIDTVPVPDASKWSVPPFEGKIVDGKIYGRG
                     TSDMKGQYTAMMSAVAYFAKDTNRDFAGELYVAGVVHEEIFEGVSAREISKAVQPDYV
                     VIGESSELNLKIGQRGRGEIVVETFGKPAHSANPEKG"
BASE COUNT         3928 a         1222 c         2151 g         3180 t
ORIGIN      
        1 aaatttaaaa gaatttaaaa aaataaatgc tttaataaat tatcaaatac taacaaatat
       61 aataaaaatt atatatgtaa aacaattatg ttaaaaaatt ggcattataa ttgcttcata
      121 ttaagttgag atgacaaaat aaaaaaagaa aggatgttgt gtgagataat aataactgtc
      181 attaaaaaat agttgggagt gataaccttg agtaaatatc aaaagaatgt ttctatgttt
      241 gatattgatg gaaggccacc aattttaaag tgtttgccac tatcaatgca acatatacta
      301 gcaatgatag ttggaactgt tacagtacct atagtcgtag gaggagctgt gggtgttagc
      361 cctacacaaa taacactttt agtgcaatat gcattaataa tagctggtat atcaacactt
      421 atacaactat atccaatagg aaatattggt tcaagattgc ctataatttt tggagttggt
      481 tttacttatg taccaacact tgtagcagtc ggaggaaatt atggattagc aagcattttg
      541 ggtgcacaat taataggggg agtggctaca gtattaatag ggatattcat aaaaaagata
      601 agaaaatttt ttccgaatat agttgcagga acagtggttt ttacaattgg attatctttg
      661 tatcctatag caataaatta tatggcaggt ggtgtaaata gtcctactta tgcctctatg
      721 caaaactggg cagtagcggc tataacatta gtggtagtta taggtcttag tcaatttgca
      781 aaaggatatg caaaattggc agcaatattt ttgggaatta tagttggata ttgtgtatct
      841 ttagttctgg gtataataaa ttttgaccct attagagagg caacttggtt tgcgatacct
      901 aaaccgcttg aatttgggat ggaatttgat atgcctgtaa taatctcagt agtaattgta
      961 agtataatca atgctgttca agcaattgga gatttatcag ctactacaat gggaggcatg
     1021 gataggaata tcacagataa ggaattatca agtggagtaa taggaagtgg aatttgtact
     1081 atgataggag ctttgtttgg aggtttacca ccatcatcat atagccaaaa cgtggggatg
     1141 gtagctatga acaaagtaat aagtagattt gtgcttggaa tagctggtat atttatgtta
     1201 ttgtcggggt ttgtgcctaa atttggtgca ctaatgacta ctataccata ttctgtttta
     1261 ggaggagcta caatatctat atttagtatg ataacaatga caggtataaa gttaattatt
     1321 acagaagagt tgtcatcaag aaatattacc attgttggtt tggcaattgc cttaggcatg
     1381 ggaataacat cagtaccagc agcccaagag gcatttcctt catttgtaaa gttagtattt
     1441 ggagaatctc caatagtaat tgcaacattg gttgcattcg ttttaaatct aataatacca
     1501 aataaaagtc tagaggatga ggctaaagaa agagctgtta tggaagagac taattaagat
     1561 ataataattt aaaagaattg tttaaattat ttagtatgag attgttagat tatttttgat
     1621 agctattcat tttaataaaa aatttaacaa ataaatatat accaggcaat taatgttcat
     1681 ttaagttgaa aatcggggag ggaatattta gaaatttgtt aattttttat aaaaatagct
     1741 cagtttagct gtgtttatcg gttgagcaat aaattcatgg tcataaaaat atttatagaa
     1801 aataattata aaaatattta gtcaagatat ttatgtatgg gaggatttat tttatgaaaa
     1861 cacgtaatac ttcaaaatat gatgtcgatg ggataccacc actaaaggaa tcaatacctt
     1921 tggccttaca acatctgtta gcaatgatag taggaaatat ggtacctgcg atattaatag
     1981 caaacgtggt aggacttaac caggggcaag caacaatgtt gattcagggt tctatgttag
     2041 cagcaggttt agcaacgttt ttacagttat atccaatacc tttatttaaa ggatttaagt
     2101 taggttcaag attacctgta atgatgggaa tgagttatgt atttttagga gcttgtcttt
     2161 cagtagcagc taaagatggt ttagcagctc tatttggagc acagattgct gctggggtaa
     2221 tcgtattctt tgtaggattt ggagttaaga aaattagaca tatattcact ccaatagttt
     2281 ctggaactat aatagcatgt atgggacttg gactattcgc aacagctata aagaatttag
     2341 ctggaggaga aggcacacaa acatttggaa gcccaataaa cttcacagta ggagttatag
     2401 ttgcttttgt aataataatg ataaataaat acggtaaagg tttagtaaaa aattcttcaa
     2461 tattaatagg aatattagta ggttatgcta tatctttagt tttaggattg gttgatttct
     2521 cagccgttca aggagcagct atagtatctt taccaacacc agcagctttt ggattagaat
     2581 ttagaccaga gttaatagta atgtttacta ttatttacat aataggtata gctgatatga
     2641 tgggtgcctg tacaatagct acagtaggag caatggatag agaggtaact gacgaagaat
     2701 tagcatcagt tgttttagga aattctataa cttctataat ttcatcacta tttgcagctc
     2761 ttccaactgg agtatttagc caaaatacag taattgtatc tatgaacaaa gttacaagta
     2821 gatttgtaat agcactaggg gcacttgtat tattactagc agggatatct ccagcattgg
     2881 gtgcaataat gacaactata ccatcttgtg ttgttggtgg agcaacttta gtagttttct
     2941 catcaatagc aatgtctggt ttctctatta tgagtatgga tggattcact gaagaaaaca
     3001 atcttatagc aggtgtatct atagcaacaa gtatgggtct tacaactgct ccacaagttt
     3061 tagaccaatt cccagaaaca attggaactg tccttggagg gtcaagtata gtttctggag
     3121 caataatagc tatattactt caaacattat ttaaattaaa gtcacgtaaa agtgctgaaa
     3181 acgttacttc aaatcttgag gaaaatattg gataaatgta ttagatataa tatgagaaaa
     3241 ccatatggta aaatgttgta atgaaagtat tcaaaggggg atttaagatg attgatatac
     3301 taataaaaaa tgcaataatt gttacagtta ataaagacag agatgttata tttgatggag
     3361 caatagctat tcaaggtgac agaatcttgg acataggaaa tacagttgat attgagccta
     3421 agtatcttga tgctaaaaaa gttatagatg ctaatggaaa agtaatattc cctggcttta
     3481 taaatactca taatcacctg ttccaagtgt tattaaaagg tttaggtgat gatatggcac
     3541 ttcatgagtg gttaaataca atgatgtttc catcagcaaa attcttaaca gaacaagata
     3601 cttatgatgc tgcaatgctt ggatgtatgg aaggtttaaa aagtggtata actacaatgg
     3661 ttgattatat gcatactcac aatagaccag gacttacaga tggaatagta aaggcatata
     3721 aagatttagg tataagaggg gttgtaggta gaggttgtat agatttaggt attcacaaag
     3781 agcttataga agatgtggaa acggttgaaa aagatttgag aagggtattc gaaaaatatc
     3841 ataattcaga aaatggaaga ataaaaatct gtgtagctcc ttcttcaatg tgggctatat
     3901 ctgaagaaat gggtaagatg ctttggaata tagttaaaga atatgattca tattttacag
     3961 ttcatatatc tgaaacagaa tttgcaagaa cagcaacaaa ggatatacat ggagaaatag
     4021 atataaaatt acttgaaaaa tggggtatag taggtccaga agttgtagca gtacattgtg
     4081 tatgtataac tgatgaagac atggaaatgc ttaaaaagta tgacataaaa gtttctcata
     4141 atgtagcaag taacatgtat cttgcttctg gtgttgcacc tgtgccagaa atgttaaaaa
     4201 aaggtataaa tgtgagttta ggtcttgacg gtgcagcaag taataacgca caagatatgg
     4261 tagagcttat gaaattaaca gctttacaac acaaagtaaa taattgtgac cctctggcaa
     4321 tttctgcaga gaaagttctt gaaatggcta caattgaggg agcaagaact cttagaatgg
     4381 aagatgaaat aggttctttg gagattggga aaaaagctga cctagtaata tttaactcaa
     4441 tgctttctcc aaaatcaata cctcttcaca atcctgtatc aactttggta tactcagcta
     4501 gtatgcacaa tgtagagagt gttttagttg atggaaatgt aatacttgaa aatggaaaag
     4561 taactacaat tgaaagtgaa gaacaagtgt tgcttaatgc acaacatgca gcagaagaat
     4621 tatgtaagag agctaatgta actaatagaa tggaaggaca taagtggaat agcttatact
     4681 aatattaaaa taagattgta ataatattct attacaacat tagtttatct gtaggaggag
     4741 aaaatggaaa aaataaaatt aaatattaat gattacatgt atgaaataga agttgaaaag
     4801 aatgaaactc ttttgcatgt acttcgtgaa aaattaaatc taactggagc gaaacaaggt
     4861 tgtaatagtg gcagttgtgg agcatgtaaa gttttgatag atggagttga catgaaatct
     4921 tgtaaacttc ttgcaaaaaa ctgcgaaggt aaagttatat acactataga aagttttgct
     4981 aatggagaaa atcttcatcc aatacaagaa gcttttatag aagtcggagc tgtacaatgt
     5041 ggatattgta ctcctggtat gattataaca acacaagctc ttttaaatag aaatgcatct
     5101 cctaatgaag atgaaattag agaagctttt aaagaaaatc tctgtagatg cacaggatat
     5161 gtaaagatta tagaagctgt aaaattatct gcagctagat tagggggtag ataattatgg
     5221 aaaaggtaat aggaaaatca taccctctaa aagatgcagc tttaaaagtt actggtcaaa
     5281 tgaaatacgt ggctgatatg aaaccacaaa atgtacttta cgctaagatg cttttaagcc
     5341 ctgtagcaca tgcaaaaata aaaagtatag atacaagtga agctgagaaa gtagtaggtg
     5401 ttagagctat agctacttat ctcaatacat cacaaagaaa atacaatggt tcattaagat
     5461 tttatgagca caaaatacct gaaaatgaag ttatattttc agatacagtt agatttgttg
     5521 gtgaccgtgt tgcagcagta gctgcagaga ctccagaaat agcagagaaa gctgtaaaac
     5581 ttataaaagt tgaatatgaa gaattgccag ctgtatttac tatacaagat gcaataaaag
     5641 aagatgctat ctgcatacat ggagattcaa atatagtttc tcaaaacaac ataaatgggg
     5701 gagatgtaga aaaaggcttc aaggaatgtg attatatatt tgaagatgaa tactctactc
     5761 cagcaataca tcatagtgca atagagactc atagtgtaat tgcacactat gattataatg
     5821 ataaattaac tgtacataca ccttgccaaa atacatttgg atttagaata atactatctc
     5881 aaatttttgg gatgtcatta aataaagtaa gagttataag acctgctatt ggaggctcat
     5941 ttggtggaaa attagaactt acaattgaac cagtagcagc cgcattatca aaaatgacaa
     6001 gaagaccagt taaaatggtt cttacaagaa aggaatctat gatatcaaca agaactcgtc
     6061 atgggtcttt tgtaaaatta aaaactggtg taaataaaga tggtgaagta atagcacaag
     6121 acataaaaat atatacaaat acaggagcat atgcatcaag tgctttaaat gttataggag
     6181 cacttagtca taaagtattt aaagtttata aaataccaaa tataaagttt actgggatgc
     6241 ctgtatatac aaatacacca atagcaggag ctatgagagg ctatgggtca cctcaaatat
     6301 ttatggctca acaagctcaa tttgcgaaaa tagcaaagga aataggtatg gatttagtcg
     6361 attttcaaaa taaaaatgca gtagagccag atgatgtcga tataatcttc cacggttctc
     6421 ttggaaatcc tagagtattg gattgtatag agcaaggtaa gaagatgttt aagtgggatg
     6481 aaaagaaaaa acttccaaaa gaagaaggaa aatatttaag aggtataggt atggcaatag
     6541 gtgcacatgg taatggagtt tttggtgcac atagagatgt tgttacattg actcttaaat
     6601 taaatgaaga tggtacgttg acactactaa caggagctca tgatatgggt aatggtgttg
     6661 taactatgca aacaatgatg gtagctgaag ttttaggaat aacacctgat aaagttgata
     6721 catttgaaac tgatacggat gcatgtacat tcaaccttgg tgactatgca agtagagggg
     6781 tatttgtaga aggtggagga gctaaaaaga cagcagagaa attgaaaaat cttatattag
     6841 aagaagcaga aaaattactt gaaacatcaa aagaagattt atatctagac aatggatgtg
     6901 taattagtaa acttgatgaa aatgtaaaag catcactttc tgatgtcgca gtatatagtc
     6961 aaagtaaatc attaagagag ttaactgttg ttgaagatta ttcttcacca gctggacgta
     7021 cttcttatgg tgtacatttt gctgaagttt tagtagataa agaaactaag gatataaaag
     7081 tagttgattt tgtagctgta catgatgttg gtagagttat aaatcctcta aatttagaag
     7141 gacaattaga aggtgggatt catatgggtc taggatacgc attgagtgaa gaattggcat
     7201 ttgatgaaca aggtagacct aaagctactg attttaaaac atataaatta tttagaactg
     7261 tagatatgcc aaaatgtcaa gttgcatttg tagatggtta tgaaaaagct gggccttttg
     7321 gtggtaagag tattggagaa tgtgccgttg taccagttgc gccagctgtt gcaaatgcag
     7381 tatataatgc aactgatgta gagatacatt cattaccaat aaaattaaaa tagtagaaat
     7441 taaagcagaa ataacaattt attgtatttg taaatagata ataaaaatag atttatttgg
     7501 tgaggagata ttattatggc aaagatatta ggtatttgtg gaagtccacg taaaggagca
     7561 acagaatatg cagtgttaga agctttgaaa gaagcagaaa ctatacctgg tatagaaact
     7621 gagtattggt cagttagagg gaaaaaaata agtccttgtg tacattgtga tgcatgtata
     7681 agaaaagaaa ctatgtgtat tataaaagat gatatccaag aattagagca aaaaatattg
     7741 gaggcagatg gaattatagt aggttctcca gtttatgata tgaatataac tgctcaactt
     7801 acagcagtat ttaatagact tcgtccaatg tatttagttc atccaggaaa acttcaaaat
     7861 aaggttggtg cagctataac tactggtgga actagatatg gtggccaaga attgacaaaa
     7921 cttcctataa taaatttctt cctaatgcat gaaatgttag tgtctggagg tttaggtgga
     7981 tgctatatag gtggtacaat atggagtaaa gatggaaaag caaaaggtgc ccaagaggat
     8041 gagacaggta tggacacagt aaaaagactt ggaaaaggtg cagcagaggc agttatggtt
     8101 tctaagtttg gtcttgaaaa atggaatgtc gtaaaagaag aattagatgt taaaaaagat
     8161 gaaaaatcac ctcttagaga ccactaaaga tttattatat tattgaatat atacaaatag
     8221 tgatgtaata gattattgca tcactatttt atgttttaat ttagatttta taaattttat
     8281 attaaaaaat atagaaacaa agtgttttga gatttatttt taaattttaa aaatgagaat
     8341 taaaaagtga gaagatagaa aaggtttttt agttattaaa ttctacatac attgtatgaa
     8401 ctaaagatag aaaagaaaaa ataataaaag cattgcaatt tctatactaa agagtcatgg
     8461 aaaaatatct agtatatatt tagtattggc atgttaattg cttatatatt aaagtataaa
     8521 aatttaacaa taatttggag gtattttaga tgttaaaaga aattaaatgg aaagtaaata
     8581 atttgccaaa gggcgataag gaaaattgta tcaaattttt aaatgaagaa gaaataacta
     8641 aggttagaaa ttttcacaaa agctttcctc aatataaaga aactccatta gcaaacctag
     8701 aaggtttagc aaaaaaatta ggtgttgcag gagtatatgt aaaagatgaa tcttatagat
     8761 ttggattaaa tgcatttaaa gtattaggtg gttcttattc tatgggtaga tacttagctc
     8821 aaagattaga tacagatata tctgagttag ggtatgacaa attgacttcg aaagaaataa
     8881 aagaaaaatt aggagaaata actttcttta cagctacaga tggaaatcac ggtagaggag
     8941 ttgcttggac agctaataaa ttaggtcaaa aatctgttgt attaatgcca aaaggttctt
     9001 ctgaatttag attaaataaa attaaaggag aaggtgcaga tgctagtata acagacttaa
     9061 attatgatga tgcagtaaga ttagctaatg attatgcaga agctgatgac catggagtaa
     9121 tggttcaaga tacagcatgg gacggatatg aagaaatacc agcatggata atgcaaggat
     9181 atggaactat ggctcaagaa gctatagagc aattaaaaga atatggagta gatagaccaa
     9241 cacacgtatt tgttcaagca ggagttggtt cacttgctgg tgcagttcaa gggtatgttg
     9301 cttcaatata tgatgaatgc ccaataacag tagtagtaga ggctgatgaa gctgattgtt
     9361 actataaatc agcagaagct ggagatggaa aaccaagatt tgttggagga gatatgccaa
     9421 ctataatggc agggcttgct tgtggagagc caaatactat aggatttgaa gtattaaaaa
     9481 atcatgcagc agcatttgtt tcagcaccag actgggtttc tgctaaaggt atgagaacat
     9541 taggtaatcc tttaaatggt gatgaaaagg taatttctgg agaatctgga gcagttacaa
     9601 ctggtctttt agtagcagct atggaaagag aagatttagc agatttaaga aaagatttaa
     9661 aattagatga aaattcaaga atacttttaa taagcacaga aggagatact gaccctgaca
     9721 aatatagaag tatagtttgg gatggagaat atccaagtat ataaggaatt actaaaatgc
     9781 aattattaga tattggttta catatttaga agatatataa gttctaaaat ttagaaaata
     9841 tataagtata taaaatacat acattataaa aaatctaggg ggataatgaa atgttaaacg
     9901 aacaaagaaa acaagaagta actgaaatat gccaaaaatt aattcaaaat cctagttctt
     9961 ctggaaatga agaaggtgtt gtaaaagcaa tagaagagtc ttttgaaaaa ttaggttttg
    10021 atagttggtc aagagataga tatggaaata tagtaggatg tataaaaggt aacaagccag
    10081 gtaagaaaat attatttgat ggacatatag atacagttcc agttccagat gcaagtaaat
    10141 ggagtgttcc tccttttgaa ggaaaaatag ttgatggaaa gatatatggt agaggaactt
    10201 ctgacatgaa aggtcaatat actgcaatga tgtctgctgt agcgtatttt gctaaagaca
    10261 ctaatagaga ttttgctgga gaactatatg tagcaggtgt tgttcatgaa gaaatatttg
    10321 aaggtgtttc ggctagagag ataagtaagg ctgttcaacc tgactatgtt gtaataggtg
    10381 agtcttcaga gttaaactta aagattggtc agagaggtag aggagaaata gtagtagaaa
    10441 cttttggtaa accagctcac tctgctaatc cagaaaaagg t
//
LOCUS       DAYAPA010000139        10357 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.143, whole genome shotgun sequence.
ACCESSION   DAYAPA010000139 DAYAPA010000000
VERSION     DAYAPA010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10357
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.143"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            519..1679
                     /locus_tag="ACGI59_003279"
     CDS             519..1679
                     /locus_tag="ACGI59_003279"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HFG9741049.1"
                     /translation="MKKEMLAMILAGGQGSRLGVFTKRIAKPAVSFGGKYRIIDFVLS
                     NCSNSGIDTVGVLTQYRPLILNSHIGMGSHWDLDRINGGVYVLQPFMNEKEGNWYNGT
                     AHAIYQNMDFVDTYNPEYVLILSGDHIYKMDYSKMLKFHKEKGSKATIAVIEVPWDEA
                     SRFGIMNTNEDSSIYEFEEKPSEPKSNLASMGVYIFDWKMLRNYFKEAEKNPEINYDD
                     FGKNLIPKMLEDNVGMYAYPFKGYWRDVGTIQSLWDANMDIIKSPETLDLADPKWKIY
                     TNTMAMPPQYIGKNANVHRSMIADGCRILGEVGNSVLSHGVVVGKGSKVIDSVIMPNV
                     VIGENVTIEKAMIGECATINDNVQIKNVNNEINVVSEYENIEPRCVLIEGGL"
     gene            1681..2811
                     /gene="glgD"
                     /locus_tag="ACGI59_003280"
     CDS             1681..2811
                     /gene="glgD"
                     /locus_tag="ACGI59_003280"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HFG9741050.1"
                     /translation="MRNECLGIINLNKKGDPAINKLNYGRPIASTPIAGRYRIIDFAL
                     SNMINSGITKVGIFAKEKYRSLTDHIGSGKDWDLSRKKGGLSIFSPENTKYRNTYSHR
                     EGDIYTILANLDYIEKSEEEYILIAPSYMLCNLNYSQALEYHKKSHNDITIIYKNVNN
                     ANKDFAGNLTLNLDSNNRVINVGNNLGKFPRANICMETYIMKREDFVECIYNIVNKGN
                     YCYLEEFIIEEAENMKIGAYEYTGYLKCVNSVESYFEMSKDLLNLEVADELLYSERKI
                     FTKEKNESPTIYTDSAKVENSFIASGCLIEGTVKDSIIFRKVNVEKGTVIENSIVMQN
                     CVIKSNAKLYNAILDKNTSVSNGKELRGDEKYPLVVEKNTNI"
     gene            2840..4282
                     /gene="glgA"
                     /locus_tag="ACGI59_003281"
     CDS             2840..4282
                     /gene="glgA"
                     /locus_tag="ACGI59_003281"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HFG9741051.1"
                     /translation="MKVFYVTAECWPFAKTGGLGDVSYALPKELKKEGVDVRVIMPKY
                     STIPSYLKDQLKEIAVFSVRVGWRNQYCGLLEMELDGVKFYFIDNEFYFRREDERKSI
                     YGYGDDAERYTFFTDAVLEAISRIDFYPDVIHINDWHTGMLPLILKERYATLEGYKNI
                     KTMYTIHNLQYQGVFDKHVLYDILDLPQKYFDNGDIEYYGSINFMKAGINFADKIITV
                     SPTYANEIQTSFYGEQLDGLLRKESGKLKGILNGIDYDLNDPAKDKDIFVHYDVDSID
                     KKVENKLRLQDILGLKKDSSIPLIGIVSRLVSQKGFDLIAYMMPELMREDLQIVVLGT
                     GEHQYQSMFNYYDSNFSDKVSARITFNASLAQQIYAASDMFLMPSLFEPCGIGQMLAM
                     RYGSLPIVRETGGLRDTVTPYNKFTGEGNGFSFKNYNAHEMFFCLKNAIKVFKDKEKW
                     IKLVENAMKTDNSWKKSAKEYIETYRDICD"
     gene            4296..6716
                     /locus_tag="ACGI59_003282"
     CDS             4296..6716
                     /locus_tag="ACGI59_003282"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HFG9741052.1"
                     /translation="MVTISKKKFKQKFEVKMYSLYAQSIKEATDEQLLNVLCSLLKDE
                     IAKKWVATKLDKKKEVYYFSLEFLIGRQLKSNLLNLNIEEEVREGLSELGINLDDLIE
                     AEVDPALGNGGLGRLAACFLDSMASLNISGQGYGIRYKYGLFEQKFVNGYQVEVPDNW
                     LTEGRYAWETVRPNEATMVKFGGEVELIKEGSHLKVIHKNYLPVMAMPYDIPIIGYQN
                     QCINTLRLFKSEIPKRDFGELTSNALNYSGSYEEALKHKYYTEEISQVLYPDDSNYAG
                     KLLRLKQEYFFVSAGIQDIIRKYKKNKLNINNLFDKVAIHINDTHPTLCIPELMRVLL
                     DEENLSWDEAWEITKKTVSYTNHTIMSEAMEKWPVSMMKELLPRIYMIIEEINRRYVE
                     ELNNKGYDQDRIKRMSIIDCDNINMANLCIVTSHSVNGVAKLHTHILETEVLKDFYQD
                     EPNKFNNKTNGIAHRRWLISSNPQLSNLITDLIGDSWKKDTLQLKNIEKFKNDSSVLQ
                     RLDDIKYNNKANLAKFIKDKYDLNVDPSSIFDVQVKRLHAYKRQLLNIFNVLHMYHEL
                     LDNPNLNLDPRTFIFGAKAAPGYYLAKCIIKFINSVASTINNDVRVKDKLKVVFLENY
                     GVSLAEIIIPAANVSEQISTTTKEASGTSNMKFMMNGAITLATLDGANVEICEQVGKE
                     NMFLFGLSAEQVLNYNKYGGYSSLDLYHSNMDIKRVVDDLINGFIPNLGEEGRSIYNS
                     LTTYNDEYFVLRDFENYGQAQADINRLYRDKEKWNKMSLVNIANSGFFSSDRTISEYA
                     KDIWFKRV"
     gene            6721..8586
                     /locus_tag="ACGI59_003283"
     CDS             6721..8586
                     /locus_tag="ACGI59_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HFG9741053.1"
                     /translation="MQSVIEYNSWDKNFKAPFGALKFDEELTICVKVNEGYNIKSISL
                     EINREEEMRTITLNEELDNDKLGKCFCGKIEKFDGTGVYFYYFKVDVEMDGQIKTLFY
                     GKNRDNGYSCEYNYSDINKYQITVYKDFKVPTWYKEGILYHIFVDRFNNGNRSGKVDN
                     PKKNSFIYGNWEDTPMYIKDSQGDVIRWDFHGGNLRGIINKLGYLKKLGVSILYLSPI
                     FEASSNHKYDTGDYKKIDPMFGDEDTFKELIDKAKEKGISIVLDGVFSHTGADSKYFN
                     MYGNYNSLGAYQSKESPYYSWYMFEEFPQKYKSWWDVKTLPNINELEHSYMDYIIYDN
                     DSVINKWVNMGIKGWRLDVADELPTKFIRELKKELKKADDDSILIGEVWEDASNKISY
                     GQRRSYLLGEELDSVMGYPFRNNMFSFLKGEINSYELCNRYMQIKENYPKESFRSNLN
                     LIGTHDVTRAKTELNDDVDLVKLAVAIQMTFEGVPYIYYGDEAGLCGGVDPDNRRTYP
                     WKNEDEDMLNFYRDVIRIRNKNKLLSSGNTEFIYTNNDSVFSFIRVNENNDRMLILVN
                     RSENVESISLCIESSFIEEIPIKYSLKNANSTIQIENNELKVDLDSKSFRIFRVN"
     gene            8831..9484
                     /locus_tag="ACGI59_003284"
     CDS             8831..9484
                     /locus_tag="ACGI59_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFG9741054.1"
                     /translation="MKVDPLTTQVYDYISKKIQNGEYEANQRITESEICKSIGVSRTP
                     AREALTRLAGENLLEKIPNKGFVVKEFQEKEKLDTYSVIGVLDALAGSSALQNLTESD
                     LVKMEELTEMMAVSIKYKNYNSYLKLSNEFHDIYITKSDNQVLVNLLNSLRYNFMSKS
                     YTSDNEDELYKMLTYSNNQHIEVVKSMRENDLENVERILREHWKTIPMEEMKKEKLL"
     gene            9580..10179
                     /locus_tag="ACGI59_003285"
     CDS             9580..10179
                     /locus_tag="ACGI59_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFG9741055.1"
                     /translation="MYPNKEIAIEELEVAGKMNPGPWIKHSYNVANVAKLIAEQCENL
                     NSEKAFVCGLLHDIGRRTGISSVRHIIDGYDYVISKGWDEVARVCLTHSFPVKDIEAD
                     IGNKDISKEQYDFINHYLNNLEYNDYDKLIILCDALADANGFCILEKRFVDTTRRYGI
                     YPFSVERWNKTYEYKEYFEKIINKSIYTLLPNIEKSIYY"
BASE COUNT         4167 a         1005 c         1872 g         3313 t
ORIGIN      
        1 atattattaa tatatattta taaaaatata aacattaata ctaagaaaac taataaataa
       61 tttaaaagtt tatatccttt tgtttaaatt atagacaaag aagtatcaat taaaaaataa
      121 agttgattaa taattacttg attttccttg gataattatt taacaaatat tagattatat
      181 tttgctatta cgaatttaac aaattaaaaa ataagcatgt tcatatgaca caaaggattt
      241 ataaaaaatg tttttatatt tataaaataa aatttgtatt attttttaac cgataattga
      301 atattaataa atactatagt gcttatggaa aataatgtat acgtcggtaa gtccattatg
      361 gggtatacat tatatcaatt gtatagttga tattatttta tttttattaa ggtattattt
      421 aagcaacatt tatacacgac atttattgac tgataaagtt tataaaagta aaaatgcaat
      481 tataaatatt aattactaga tgaggggagt gtcattaaat gaaaaaagag atgttagcta
      541 tgattttggc aggagggcaa ggttcaagat taggtgtttt tacaaaaaga attgccaaac
      601 cagctgtatc atttggaggt aagtatagaa ttatagattt tgtgttaagc aattgttcaa
      661 actctggtat tgatactgta ggtgttttaa ctcaatatag accgttgata ttaaattcac
      721 atataggcat gggaagtcat tgggatttgg acagaataaa tggtggtgtc tatgttttac
      781 aaccatttat gaatgaaaaa gaaggaaatt ggtataatgg tacagcacat gcgatatatc
      841 aaaatatgga ctttgtagat acctataatc cagaatatgt attgatatta tctggtgacc
      901 atatatacaa gatggattat agcaaaatgt taaaatttca taaggaaaaa ggctctaaag
      961 ctacaatagc agtgattgaa gttccttggg atgaagcttc aagatttggg ataatgaata
     1021 caaatgaaga ttcaagtatc tatgaatttg aagaaaaacc aagtgaacct aagagcaatt
     1081 tggcatctat gggagtatac atatttgatt ggaaaatgtt gagaaattat tttaaggaag
     1141 cagaaaaaaa tccagaaata aattatgatg attttggaaa aaatttaata cctaaaatgt
     1201 tagaagataa tgttggaatg tatgcttatc catttaaagg atactggaga gatgtaggaa
     1261 ctatacaaag tctatgggat gctaatatgg atataataaa atctccagaa acacttgatt
     1321 tagcagaccc taagtggaag atatatacaa atactatggc aatgcctcca caatatatag
     1381 gtaaaaatgc aaatgttcat agatcaatga tagctgatgg ttgtagaatt ttaggtgaag
     1441 taggtaactc tgtattatca catggagttg ttgttggtaa aggaagtaaa gtaattgatt
     1501 cagtaataat gccaaatgtt gtaataggtg aaaatgtaac tatagaaaaa gctatgatag
     1561 gtgagtgtgc aactataaat gataatgttc agataaagaa tgtaaataat gaaataaatg
     1621 tagtttctga gtatgaaaat atcgaaccta gatgcgtatt aatagaagga ggtctttaag
     1681 atgagaaatg aatgtttagg gataataaat ttaaacaaaa aaggagaccc agcaataaac
     1741 aaacttaact atggaagacc aatagcatct acaccaatag ctggaagata tagaataata
     1801 gactttgctt tatctaatat gataaattca ggaatcacta aagttggaat attcgcaaaa
     1861 gaaaaataca gatcattaac agaccatata ggtagtggta aagattggga tttaagtaga
     1921 aaaaaaggtg gcctatcaat ttttagtcca gaaaacacta agtatagaaa tacttactca
     1981 catagagaag gtgatatata tactatctta gctaatctag attatataga aaagagtgaa
     2041 gaagaatata ttttaatagc tccaagctat atgctatgca atttaaatta ttctcaagca
     2101 ttggaatacc ataaaaaatc tcataatgat ataactataa tatataaaaa tgtaaataat
     2161 gcaaataaag attttgctgg taacttaact ctaaatttag atagtaacaa tagggtaata
     2221 aatgtaggaa ataatttagg taagttccct agagcaaaca tatgtatgga gacatatatt
     2281 atgaagcgtg aagattttgt tgaatgtata tataacattg taaataaagg caattattgt
     2341 tatttagaag aatttattat tgaagaagca gaaaacatga aaataggagc ctatgaatat
     2401 acaggatatc taaaatgtgt aaattctgtt gaatcatatt ttgaaatgag caaagattta
     2461 ttaaatttag aagttgcaga tgaattatta tattctgaaa gaaagatatt tacaaaagaa
     2521 aagaatgaat cacctacaat ttatacagat agtgctaaag tagaaaatag ctttatagca
     2581 tctggttgtt taatagaagg tactgtaaaa gatagtatca tatttagaaa ggtaaatgtt
     2641 gaaaaaggaa cagttataga gaactcaata gtaatgcaaa attgtgtaat aaagtcaaat
     2701 gcaaaactat ataatgcaat attagataaa aatacaagtg tatcaaatgg gaaagaatta
     2761 agaggagatg aaaaatatcc tcttgtagtg gaaaagaata caaatatata aataaatttt
     2821 ttagaagggg gaatgactaa tgaaggtttt ttatgtaaca gcagagtgtt ggccctttgc
     2881 aaaaactgga ggattaggag atgtttctta tgcattgcct aaagaattaa aaaaagaagg
     2941 tgtagatgta agggtaataa tgcccaaata ttcaactata ccaagttatt taaaagatca
     3001 actaaaagaa atagctgtgt ttagtgtacg tgttggatgg agaaatcaat actgtggtct
     3061 tttagaaatg gaattggatg gtgtaaaatt ttactttata gataatgaat tttattttag
     3121 aagagaagat gaaagaaaga gtatctatgg atatggagat gatgcagaaa gatatacttt
     3181 ctttacagat gcagttttag aagctataag tagaattgat ttttatccag atgtaataca
     3241 tataaatgat tggcatacag ggatgttacc attaattcta aaagaaagat atgcaaccct
     3301 tgaggggtat aaaaatataa aaactatgta tactatacat aatctccaat atcaaggtgt
     3361 atttgataag catgttttat atgatatatt ggacttacca caaaaatact ttgataatgg
     3421 agatatagag tattatggct caataaattt tatgaaagct ggtataaatt ttgcagataa
     3481 aattataact gttagtccta cttatgcaaa tgaaatacaa acatcttttt atggcgaaca
     3541 attagatgga ttacttagaa aagagtcagg aaaattaaaa ggtatattaa atggtataga
     3601 ttatgattta aatgaccctg caaaagataa agatatattt gttcattatg atgtagactc
     3661 aattgacaaa aaagtagaaa ataaattgag attacaagat atattgggac taaaaaaaga
     3721 ttcatctatt ccgttgatag gtatagtttc gagattagtt tctcaaaaag gatttgattt
     3781 aatagcatac atgatgccag aacttatgag agaagattta cagattgtcg ttttaggaac
     3841 tggagaacat caatatcaat caatgttcaa ctattacgat tctaattttt cagataaagt
     3901 atcagctaga attactttta atgcatcatt ggctcaacag atatatgctg caagtgatat
     3961 gttcttaatg ccatcattat ttgagccttg tggtataggt caaatgttag ctatgagata
     4021 tggttcttta ccaatagtta gagagactgg agggcttaga gatacagtaa caccttataa
     4081 taaatttact ggagaaggca atggatttag ttttaaaaat tacaatgctc atgagatgtt
     4141 tttctgtttg aaaaatgcaa tcaaagtatt taaggacaaa gaaaaatgga ttaaattagt
     4201 tgaaaatgca atgaaaacag ataatagttg gaaaaaatca gctaaggagt atatagaaac
     4261 atatagagac atatgtgatt aaaggagtgg tttttttggt aacaataagc aaaaagaaat
     4321 tcaagcaaaa atttgaagta aaaatgtata gcttatatgc acaatctatc aaggaagcta
     4381 ctgatgaaca attgttaaat gtattatgta gtctattgaa agatgagata gcaaaaaaat
     4441 gggttgctac aaagttagat aagaaaaaag aagtttatta ttttagttta gaatttttaa
     4501 tagggagaca gttaaaatct aatttattaa atttaaatat tgaagaagaa gttagagaag
     4561 gtttatctga attaggaatc aacttggatg atttaattga agctgaagta gacccagcct
     4621 taggaaatgg tggacttggt agattagcag cttgcttttt agattctatg gcatctttga
     4681 acataagtgg tcaaggatat ggaataaggt ataaatatgg tctgtttgaa caaaaatttg
     4741 taaatggtta tcaggtagaa gtgcctgata actggcttac agaaggacga tatgcatggg
     4801 aaactgtaag accaaatgaa gcaacaatgg tgaagtttgg tggtgaggta gaattaatca
     4861 aggaaggtag tcatttaaaa gtaattcata aaaactacct tcctgtaatg gcaatgcctt
     4921 atgatatacc aataatcgga taccaaaacc aatgtataaa tactttaaga ttgtttaaga
     4981 gtgaaatacc aaagagagat tttggtgaac ttacctcaaa tgctctaaat tattctggta
     5041 gctatgaaga ggctttaaaa cataagtatt atactgagga aatatcacag gtattatatc
     5101 ctgatgattc aaactatgca ggtaagttgt tgaggttgaa acaagaatac ttttttgtaa
     5161 gtgcagggat acaagatata ataagaaaat ataaaaagaa caaattaaat attaataatt
     5221 tattcgataa ggtagcaatt catataaatg atacacatcc taccttatgc ataccagaac
     5281 ttatgagagt tttgcttgat gaagaaaatt tatcttggga tgaagcatgg gaaataacta
     5341 aaaaaacagt gtcctatact aatcatacta taatgtcaga agcaatggaa aaatggcctg
     5401 taagtatgat gaaagaacta ttaccaagaa tttatatgat aatagaagaa ataaatagaa
     5461 gatatgtaga ggaattaaat aataaaggat atgaccaaga tagaataaaa agaatgagta
     5521 taatagactg tgacaatatc aatatggcaa acttatgtat tgtcacaagt catagtgtaa
     5581 atggagttgc taagcttcat actcatattc ttgaaacaga ggtattaaaa gatttctatc
     5641 aagatgaacc aaataaattt aataataaga ctaatggaat tgcacatagg cgatggctta
     5701 taagttcaaa tcctcaactt agcaatttaa ttacagattt aattggtgat tcatggaaga
     5761 aagatacatt acaattaaaa aatattgaaa aatttaaaaa tgattcctct gttttacaaa
     5821 gacttgatga tataaaatac aataataaag caaatctagc aaagtttata aaagataaat
     5881 acgatttaaa tgtagaccca agttcgatat ttgatgttca agttaagaga ttgcatgcgt
     5941 ataaaagaca gttactgaat atatttaatg tacttcatat gtatcatgaa ttacttgata
     6001 atcctaattt gaatcttgac ccaagaactt ttatatttgg cgcaaaagca gcaccaggat
     6061 attatttggc taaatgtata attaaattta taaattctgt tgcaagtact ataaataatg
     6121 atgttagagt aaaggacaag ttaaaagtag tatttttaga gaattatgga gtatcattag
     6181 cagaaataat aataccagca gcaaatgtta gtgagcaaat ttcaactaca actaaggaag
     6241 catctggaac aagtaatatg aagtttatga tgaatggtgc tataactttg gctacacttg
     6301 acggtgcaaa tgtagagata tgtgaacagg ttggtaaaga aaatatgttt ttatttggac
     6361 ttagtgcaga acaagtacta aattataata aatatggtgg atattcttca cttgacctat
     6421 atcattctaa tatggatata aaaagggtag tagatgattt aataaatgga tttattccta
     6481 atcttggcga agaaggaaga agtatttata attcactcac tacttacaat gatgagtatt
     6541 ttgtattgag agattttgaa aattatggtc aagctcaagc agatataaac aggttatata
     6601 gagataaaga aaaatggaat aaaatgtctt tagttaatat agctaattct ggattttttt
     6661 cttctgatag aacaatttct gagtatgcaa aagacatttg gtttaaaagg gtgtgattaa
     6721 atgcaaagtg taatagaata taactcttgg gataaaaatt ttaaggctcc gtttggagct
     6781 ttaaaatttg atgaggaatt gaccatttgt gttaaagtaa atgagggata caatatcaag
     6841 tctatttccc tagaaataaa tagagaagaa gaaatgagaa caataacttt aaatgaagaa
     6901 ttagataatg ataaacttgg aaagtgcttt tgtggtaaga ttgaaaagtt tgatggtaca
     6961 ggtgtttatt tttattactt taaagttgat gttgaaatgg atggtcagat aaagacttta
     7021 ttttatggaa aaaacagaga taatggatat tcttgtgaat ataattatag tgatattaat
     7081 aaatatcaaa tcactgttta caaagatttt aaagtaccaa catggtacaa agaaggaata
     7141 ctttatcata tatttgtaga tagattcaat aatggaaatc gtagtggtaa ggttgataat
     7201 cctaaaaaaa atagtttcat atatggaaat tgggaagata cacctatgta cataaaggat
     7261 agtcaagggg atgttataag atgggatttc catggtggaa atttaagagg gataatcaat
     7321 aaacttggat atttaaaaaa attaggtgta agtatactat atctaagccc tatatttgaa
     7381 gcctctagta atcataagta tgatacaggt gactataaaa aaattgaccc aatgtttggg
     7441 gatgaagata catttaaaga acttattgat aaagcaaaag aaaaaggtat atcaatagta
     7501 ttagatggag tttttagtca tactggagca gatagtaaat attttaatat gtatggaaac
     7561 tataatagtt taggggcata tcaatctaaa gaatctccat attattcatg gtatatgttt
     7621 gaagaatttc ctcaaaaata taaaagttgg tgggatgtta agacattgcc aaatataaat
     7681 gaattagaac atagctatat ggattatata atatatgaca atgatagcgt tataaataaa
     7741 tgggtaaata tgggtattaa gggctggaga ctagatgttg cagacgaatt accaactaag
     7801 tttataagag agttaaaaaa ggaattaaaa aaggcagatg atgactcaat tcttataggt
     7861 gaagtatggg aagatgcatc aaataagatt agctatggtc agagaagaag ttatttatta
     7921 ggtgaagaat tagattctgt tatgggttat ccttttagaa ataacatgtt ttctttctta
     7981 aaaggagaaa ttaattcata tgaactttgt aatagatata tgcagattaa agaaaattat
     8041 ccaaaagaat catttaggtc taatttaaat ttaattggaa cacatgatgt tacaagagct
     8101 aaaacggaat taaatgatga tgtagattta gtgaagcttg ctgtagctat acagatgaca
     8161 tttgaaggag taccatatat atattatgga gatgaagcag gtctttgtgg aggtgttgac
     8221 ccagataata gaagaactta tccatggaaa aatgaagacg aagatatgct taatttttat
     8281 agagatgtta taagaattag aaataagaat aaacttttaa gttctggaaa tacagaattt
     8341 atatatacta ataatgattc tgtattttct tttattagag ttaatgaaaa taatgataga
     8401 atgttaattt tagtaaatag aagtgaaaat gtggaaagta tatcattatg tatagagagt
     8461 agttttattg aggaaatacc tataaagtat agtcttaaaa atgcaaatag tactatacaa
     8521 attgaaaata atgagttgaa agtagattta gactctaaat catttagaat ttttagagta
     8581 aattaaatat ctttttttag aaaaataaat agaaaataag gtgaatgtgg aaagagtttt
     8641 gttttatcaa aactttttct actttttttg tataaattat cataagaatt ttagtatttc
     8701 aaaatattac aaagaagtat aaaaaatatc ataattgtga tattatataa attagtaaga
     8761 taaaactgaa ctctctacca aagttgtagg gagatttaac ttttataatc ttaatatgga
     8821 ggaacgtctt atgaaggtag atccacttac aactcaagta tatgactata tatcaaagaa
     8881 aatccaaaat ggagaatatg aagctaatca gagaattact gaatctgaga tttgtaaatc
     8941 tatcggagtt agtagaacac cagcgagaga agcattaact aggttagcag gtgaaaattt
     9001 actagaaaaa atacctaata agggatttgt agtaaaagaa tttcaggaaa aggagaaatt
     9061 agacacctat tccgtaattg gagtattgga tgcgttagcc ggttcttcag cattacaaaa
     9121 tttaactgaa tcagacctag taaaaatgga agaattaaca gaaatgatgg cggtctctat
     9181 aaagtataaa aattacaata gctatttaaa attaagtaat gaatttcacg atatatacat
     9241 aactaaatca gataatcagg tgttagtaaa tctattgaac tctttaagat ataattttat
     9301 gtcaaagtca tatactagtg ataatgaaga tgagttatat aaaatgttga catacagcaa
     9361 taatcaacat attgaagtag taaaatctat gagggagaat gatttagaaa atgttgaaag
     9421 aattttgaga gaacattgga aaacgatacc tatggaagag atgaaaaaag agaagctttt
     9481 ataaagtttc tctttatttt ttataccatt ttatcactat ttttttgaat tattaagtag
     9541 aaaataataa ttaatttaaa cttgaggagg aaaactaata tgtatccaaa taaagaaatt
     9601 gcaatagaag agttagaagt ggctggcaaa atgaatcccg gtccatggat aaaacattct
     9661 tacaatgttg ctaatgtagc taaattgatt gcagagcaat gtgaaaattt gaatagtgaa
     9721 aaggcctttg tatgtggatt attacatgac attggaagaa gaactggtat ttcttctgta
     9781 aggcatatta ttgatggata tgattatgtt atttctaagg gatgggatga agtggcgagg
     9841 gtatgtttga cacattcttt tcctgttaag gatatagaag ctgatattgg aaataaagat
     9901 attagtaaag aacagtatga ttttatcaat cactatttaa acaaccttga atataacgat
     9961 tatgataagt tgattattct ttgtgatgca ttagcagatg caaatgggtt ttgtatttta
    10021 gaaaaaagat ttgttgatac aacacgaaga tatggaatat atccattttc agttgaacga
    10081 tggaataaga cttatgagta taaagaatat ttcgagaaaa taatcaacaa gtctatatat
    10141 acattgttac ccaatattga aaaatctata tattattaaa tcctaagtta ataatttaat
    10201 atccagtatc accagcagtt ggtcattatg attgactgct tttattatgc ttatattaaa
    10261 aaaatacact atggtttgtg tatatcaaag ttatatggaa tgaaaagaga atttatttta
    10321 tcaattaaaa aatttaataa attttatatt aaaatta
//
LOCUS       DAYAPA010000140        10281 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.144, whole genome shotgun sequence.
ACCESSION   DAYAPA010000140 DAYAPA010000000
VERSION     DAYAPA010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10281
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.144"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            331..1761
                     /locus_tag="ACGI59_003286"
     CDS             331..1761
                     /locus_tag="ACGI59_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFG9741056.1"
                     /translation="MSSQESKPKGNLISNFRDDMRDPKKRAIYTYSSSINLAYNFIGY
                     FVSTVTNIFLTDVAKLGVIMAGGMQIIQSFIKIAVAPITGTVFDKQPFKKGKYYPWLR
                     YAPAALALTYGIFFIVPLMKISPALLLPLMTALLLIASCAQQVVFTIIYAIYPVVAKT
                     PRDRVIGSTTTNIFKEVGKFLVGFSYPLLLVLFADVLGGEGMGYLGTYLFFAAGCLLV
                     FWFSSTEIIKSGAEEEVLQKEEKPKIKASEMAKALFTNVPLMMAFCLEFLICIRSIGL
                     GPLAPYYFKYVIEDERGLAVFLSIMPLVSVAFMFFAPVFIKICREQKLASIISFSICA
                     LCHLLVAWTPWGKTTLGVTMLLAIGGGFSNVVSIINLNFFAGSCDYGHWKSGKDLPGL
                     SMSLYPVAIQVGVLLATTIRTVLMNSMGYQADMVVTEAVKSGFINMISYSMAIPLIIA
                     VVIAILYPVSDKKLNQIREELNQRNA"
     gene            1864..2967
                     /gene="hisC"
                     /locus_tag="ACGI59_003287"
     CDS             1864..2967
                     /gene="hisC"
                     /locus_tag="ACGI59_003287"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFG9741057.1"
                     /translation="MIEKLLRNTVKELHQYVPGEPIEKVKEKYGVKEIIKLASNENPL
                     GPSPKAIEAMIEMLKQGQLYPEPEANELRRKLAEKLDLKPENFIVANGADNVITLIGE
                     AFINRGDEVIYCNPTFPSYRTATIKNEGIPVEVPLTEDYKYDLQGILDKITDKTKLIC
                     VCNPNNPTGTIVDDKELEEFLKKVPENIITILDEAYIEFLTVPNYVDGLKYVRENYNV
                     IVTRTFSKIYGLAGLRVGYGIAKDEIIRTLFTVKEPFSANRVAISGATAALDDEEFIK
                     ETYELNRVGMAYFKEEFTKMGFDVVDSQSNFLYVDMKTDIPKLFEDLKKRGFVIRPSA
                     THARVSIGTMEENRKFVAVLKEILNIKGEPSIG"
     gene            3000..3218
                     /locus_tag="ACGI59_003288"
     CDS             3000..3218
                     /locus_tag="ACGI59_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFG9741058.1"
                     /translation="MNLKINAERCKSCEYCVISCKKGALKISSKINKEGYAHVEVDES
                     KCILCGICYQVCPDNVFEIIKEEACQEA"
     gene            3235..4284
                     /gene="vorB"
                     /locus_tag="ACGI59_003289"
     CDS             3235..4284
                     /gene="vorB"
                     /locus_tag="ACGI59_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HFG9741059.1"
                     /translation="MKKMWKGNHAIAEAALRGGCEFYAGYPITPQTEVMEFLSHRMSE
                     LGRTFIQSENEMAAIYMVYGAYASGMRSMTSSSGPGISLKQEGISYLCANHYPCVIVN
                     VQRWGPGLGSLDSAQTDYLRDTRGGGNGDYHLIVYAPNSIQETVDLMYNSFDVAEKYR
                     VPVEILTEAALGQMMEPVEFPEFKKREGDLGWTYDGSNRDHAKVADNQKPTFCMEKRM
                     RITENEQQWEDYQVEDAEYVFVAFGIPSRTTMNAVRRLREQGEKVGIIRPITVWPFPY
                     KAFEKVSKDVKGFISVESTDTGQLVEDVALASKKVCKENVPVYGLFSGNHIPKTLQVM
                     DTYSKIKSGEIKEVF"
     gene            4286..5026
                     /locus_tag="ACGI59_003290"
     CDS             4286..5026
                     /locus_tag="ACGI59_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HFG9741060.1"
                     /translation="MATEKLTPSVVSIPNKFCPGCGHGIVNRIIAEVIEEHGYEKNHV
                     LTLGVGCVCNMNFSWNGDKMQTAHGRASSTAIGVKVALPDTLVMTYQGDGDAYVIGLS
                     ETLNTAYRNHNVTVFVINNNNFAMTGGQMSWTTMPGQVTTTSVNGRDIKTTGSPLKVP
                     EMVANFFDVAYVARGSVHSPKEIINLKKYIKNAIEAQLNGEGYSLVEILAPCPTNWGV
                     SLEKSIKWMEEEIVPYYALGEFKQRDGE"
     gene            5029..5568
                     /locus_tag="ACGI59_003291"
     CDS             5029..5568
                     /locus_tag="ACGI59_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HFG9741061.1"
                     /translation="MEKELVFAGFGGQGVLTLGLIVAESALELGKQVTWMPAYGPTMR
                     GGKAYSVVKFSDESIGGPDMEEIDVLVAMNKPSLDYINLVKEGGTVVINTSAIDENVT
                     LREDISVVKINCQELAQKVNNPKAANIVVLGALIAKTGLFEKELALKTMCDFFEEKGK
                     GKFNVQNEAAFMEGYNNAI"
     gene            5692..7791
                     /locus_tag="ACGI59_003292"
     CDS             5692..7791
                     /locus_tag="ACGI59_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HFG9741062.1"
                     /translation="MSLEKLIRPKSIAIVGVTDKLGFGRSAALSIVKSKETDRVYYVN
                     PKREELFGRKCYKTIQEVPEVVDCVVVCTPRNVVPSVLKDSGELGTKAAVVYASGFAE
                     EGTEEGTDLENQLIEISNTYDMKILGPNCMGLLNCIDKVNLWAGGSKWDLDTKKPGIG
                     IVGQSGFITAEIVSSDYFNISYGFSTGNGNIVTLEDAVDFLVDDDYASVIAIYLEGLK
                     NPQKFIDALKRAAQKRKPVIILKSGRSEKGAISAASHTGNLAGSSKAFESIFEKYGVI
                     SVENLEQFMCLAQAFSVLDGNLPANSNFAAINFSGGENTICADLAEENGVELAEISSE
                     TKEEMEKYLPGFATPKNPLDATTALFHEKDMIVGLLHTFEKDDSVGFTMVGANIRDEE
                     NEMHETLCKAVSEAREQGLKKPVFAVPTLEGNRYLDYRTRLEDNQVPIMSSVGTTFTC
                     FNKMAKFIDYDYSKRTLEFKAVKKRESNNVVALSELDSKIEMKKYGVPVPGQGNAKSI
                     DELDEILKYIKYPVVLKINSSEILHKSDVGGVKIGIKNRDEAVDAYNEILTNVKKAKP
                     DANIDGILVQEMVESGIEIIIGITNDDQFGPMLLVGLGGVFVEVFKDTTLYPLPINHD
                     EAIMMLKKLKSFKLLNGYRGSEPCDIDALADMMVKLGKYAYENKDEVKEIDLNPVFVY
                     PKGKGVCAVDALIVKYK"
     gene            complement(8058..8573)
                     /locus_tag="ACGI59_003293"
     CDS             complement(8058..8573)
                     /locus_tag="ACGI59_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HFG9741063.1"
                     /translation="MISAKMEKLLNEQINHELYSAYLYLSMSSYLEAEGLKGFSNWFY
                     VQYKEETDHAMFFYKYLHNVGGKVQLDAIPMPDSDFTSAMDILERTLAHEKKVTALIN
                     NLAAVANSESDFRTSQFLLWFISEQAEEETNCEDNIKRVKLAGEGGLFFVDQEFANRT
                     YAAPTNPPVTI"
     gene            complement(8665..9876)
                     /locus_tag="ACGI59_003294"
     CDS             complement(8665..9876)
                     /locus_tag="ACGI59_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFG9741064.1"
                     /translation="MSNKQGSNIKDVKIKNKLIILKLLSTNTPMSRVDLAKSTGLTKM
                     TLSNLVTELINENMIREIESSTLNAHSSGRKPILLDISNISPCICGILIRRNICKVII
                     SDLKGNIVKQLSETYPKFLSNDVLKDIIKRMFTKIMKINKRDIIGIGISSIGPVDDIN
                     GTILNPPDFGNVSNLNIVDFIKEMSDLPIFLINDANSGALAEKMYGLGKNISNYIYLH
                     IMNGIGAGLVLENKLHTGNLGQSGEIGHTSINFSGPLCSCGNNGCLDYYTSVSNLIKR
                     IESLSHIYPNSPLINCKDFSLVKLIDEANNKDSLAMFLLDEFCTYVSYALVNTLTLID
                     CSSIIIGYDFNIPGTFIENTLLKKLTASASFSKYKKISVRHSNFGANAPLIGSIAIVS
                     YNFFNGSINFY"
BASE COUNT         3896 a         1250 c         1869 g         3266 t
ORIGIN      
        1 attattcttg aataaaatta taaaaaatta aaatcactag aaatatgata aaataattta
       61 aaatacgcat ttcaatgaca tttcaaaaca agaaaataat aaaaaacaaa cctaaattaa
      121 gtctaaatag ctttttagtt aattttagta gtaaaaaata aaacttaaaa agtgtttgac
      181 aattatgaat tttgagaata taattaaatt aaaattatac acatataaaa atatactcct
      241 gaatcaagaa tgagatattt aagattcaaa atatatatta ttatacgtga ataataaaaa
      301 gtaagtaaat tttataaggg aggggtagat gtgagtagtc aagaaagcaa accaaaagga
      361 aatcttatat caaattttag agatgatatg agagacccta agaaaagagc aatatacaca
      421 tattcaagtt ctataaattt agcgtacaat tttataggtt actttgtatc aactgttaca
      481 aatatttttt taactgatgt ggcaaaactt ggtgtaatta tggcaggtgg tatgcaaatt
      541 atacaatctt tcataaaaat tgctgtagct cctataacag gtacagtatt tgacaaacaa
      601 ccattcaaaa aaggaaagta ttatccatgg cttagatatg caccagcagc gttagcatta
      661 acatatggaa tatttttcat agttccatta atgaaaataa gtccagcatt acttttacct
      721 ttgatgacag ctttattatt aatagcatct tgtgctcagc aagtagtttt cactataatt
      781 tatgctattt accctgtagt tgctaagaca cctagagacc gagttatagg ctcaactact
      841 acgaatatat ttaaagaagt tggtaaattc ttagtaggtt tttcatatcc gttattatta
      901 gttttatttg cagatgtatt aggtggagaa ggaatgggtt atttaggtac ctacttattc
      961 tttgcagcag gatgcctttt agtattttgg ttttcttcaa cagagataat aaaatctgga
     1021 gcagaagaag aagttcttca aaaagaagag aagccaaaga ttaaagcaag tgaaatggca
     1081 aaagctttat tcactaatgt accacttatg atggcatttt gtttagaatt tcttatatgt
     1141 ataagatcta taggattagg acctttggct ccatattatt ttaaatatgt aatagaagat
     1201 gaacgtggat tagcagtatt tttatcaatc atgcctttgg taagtgtagc attcatgttt
     1261 ttcgcaccag tgtttataaa aatctgtaga gagcaaaaac tagcttcaat aatttctttt
     1321 tctatatgtg cactatgcca cttattggtt gcatggactc cttgggggaa aactacttta
     1381 ggagtaacta tgttgttggc aattggtgga ggtttttcaa atgtagtatc aataataaat
     1441 cttaatttct ttgccggttc ttgtgattat gggcattgga aatcaggaaa agacttacct
     1501 ggcttaagta tgtcactata tccagttgca atacaagtag gagtattact tgcaactact
     1561 ataagaacag ttttaatgaa ctcaatggga tatcaagctg atatggtagt cacagaagca
     1621 gttaaatcag gttttataaa tatgatttct tattctatgg caataccttt aataatagct
     1681 gttgttatag caatactata tccagtaagt gacaagaaat taaaccaaat aagagaagag
     1741 ttaaatcaaa gaaatgctta gttaaaagaa agttgagtag atggttaatt taagctatta
     1801 aagtaagtag acgcaatagc gtttatataa atgaaaatac atatacagag ggggctttta
     1861 aaaatgatag aaaaattatt gagaaatacc gtaaaagaat tacatcaata tgttccaggt
     1921 gaaccaatag aaaaagttaa ggaaaaatat ggtgtaaaag aaataataaa attagcttca
     1981 aatgaaaatc ctctaggacc atcaccaaaa gcaatagaag ctatgattga aatgttaaaa
     2041 caaggtcaac tttatccaga gccagaagct aacgaactta gaagaaaatt agctgaaaag
     2101 ctagatttaa aaccagaaaa ttttattgtt gctaatggtg cagataacgt tatcactcta
     2161 ataggtgagg cttttataaa tagaggagat gaagttatat attgtaaccc aacatttcca
     2221 tcatatagaa ctgcaactat aaaaaatgaa gggataccag tagaagtacc tttaacagaa
     2281 gattataagt atgatttaca aggtatatta gataagatta cagataaaac aaaattaata
     2341 tgtgtatgta accctaataa tccaacagga acaattgtgg atgataaaga gttagaagaa
     2401 tttttaaaga aagtccctga aaatattata actatattgg atgaagcata tatagaattt
     2461 ttaacagttc ctaattatgt ggatggactt aagtatgtaa gggaaaatta taacgttata
     2521 gtaacaagaa ctttctctaa aatatatggt cttgcagggc ttagagtagg atatggtatt
     2581 gctaaagatg agataataag aactttattt acagtaaaag agcctttctc tgcaaatcgt
     2641 gttgctataa gtggagcaac agcagcactt gatgatgaag agtttataaa agaaacatat
     2701 gaattaaata gagttggaat ggcttacttt aaagaagagt ttacaaaaat gggatttgat
     2761 gttgtagatt cacaatcaaa tttcctttat gtagatatga aaacagatat acctaagtta
     2821 tttgaagact tgaaaaaaag agggtttgta ataagaccaa gtgctacaca tgcacgtgta
     2881 agtattggaa ctatggaaga aaataggaaa tttgtagcag ttttaaagga aatattaaat
     2941 attaaaggag agccaagtat tggataatag atagaaatat tatgaaacgg ggtgtagata
     3001 tgaacttaaa aataaacgca gagagatgta aaagttgtga atactgcgtt atatcttgta
     3061 aaaaaggagc attaaaaata tcaagtaaga taaataaaga aggttatgct catgtagaag
     3121 ttgatgagag taaatgcata ctttgtggaa tttgctatca ggtgtgtcca gataatgttt
     3181 ttgaaataat taaagaagaa gcatgtcaag aggcttagaa ggagttggga taatatgaaa
     3241 aagatgtgga aggggaacca cgccattgca gaagcagccc taagaggagg ttgtgaattt
     3301 tatgcaggat atccaattac accacaaaca gaggtaatgg aatttttatc acatagaatg
     3361 tctgaacttg gaagaacttt tatacagtcc gaaaatgaga tggctgctat atatatggta
     3421 tatggagcat atgcaagtgg aatgaggtct atgacgagtt cttcaggacc aggaatatct
     3481 ttaaaacaag aagggatttc ttacttatgt gcaaatcatt acccttgtgt aatcgtaaat
     3541 gtacagagat gggggcctgg tcttggctca ttggactcag cacagacaga ctatctaaga
     3601 gatactagag ggggaggaaa tggagattat catttgatag tttatgcacc aaacagtatt
     3661 caagagactg tagatttgat gtataactca tttgatgtag cggagaagta cagagtgcct
     3721 gtcgaaattt tgacagaagc agctttaggt caaatgatgg aaccagtaga atttccagag
     3781 tttaagaaga gagaaggaga tttaggttgg acttatgatg gaagtaatag agaccatgca
     3841 aaggttgcag ataaccaaaa gccaactttt tgtatggaga aacgtatgag aataactgaa
     3901 aatgaacaac aatgggaaga ttatcaagta gaagatgcag aatatgtgtt tgtagcgttt
     3961 ggtataccat caagaacaac tatgaatgct gttagaagac ttagagaaca aggtgaaaaa
     4021 gttggaataa taagaccaat aacagtatgg cctttcccat ataaagcatt tgaaaaggta
     4081 agtaaagatg taaaaggatt tatctcagta gaatcaacag acacaggtca attagtagaa
     4141 gatgttgctt tagcctcaaa gaaagtatgt aaagaaaatg tacctgtgta tggtttattt
     4201 agtggaaatc atataccaaa aacattgcaa gtaatggaca cttatagcaa aataaaatct
     4261 ggcgaaatca aggaggtgtt ctaatatggc tacagaaaaa ttaacaccta gcgttgtttc
     4321 aataccaaac aagttttgcc caggttgtgg tcatggaata gtaaatcgta taatagcaga
     4381 ggtaatagaa gaacatggat atgaaaaaaa tcatgtatta acattaggag ttggttgtgt
     4441 ctgcaatatg aattttagct ggaatggtga taagatgcaa actgcacatg gtagagcttc
     4501 atctacagct ataggagtta aagtagcact tccagataca ttagtaatga cttaccaagg
     4561 tgatggagat gcttatgtta taggtctttc tgaaacttta aatacagcat atagaaatca
     4621 taatgtgact gtatttgtaa taaacaataa taattttgca atgactggtg gacaaatgtc
     4681 atggaccact atgccgggtc aagtcacaac aacaagtgta aatggaagag atataaagac
     4741 tactggtagt ccactgaaag ttcctgagat ggtggcaaac ttctttgatg tagcatatgt
     4801 agcaagaggt tcagttcata gcccaaagga aataattaat ctaaaaaaat atataaaaaa
     4861 tgcaatagaa gctcaattaa atggagaggg atattcctta gttgaaatat tagcaccttg
     4921 cccaactaac tggggtgtat cactagagaa atctatcaaa tggatggaag aagaaatagt
     4981 gccatattat gctcttggcg aatttaagca aagggatggt gaatagttat ggaaaaagag
     5041 ctagtatttg cagggtttgg tggtcaagga gttttaactc ttggtctgat tgtagctgag
     5101 tcagcacttg aattggggaa acaagtaact tggatgcctg catatggtcc aactatgaga
     5161 ggtggaaaag cctatagtgt agtaaagttt tctgatgaga gtataggtgg gcctgatatg
     5221 gaagaaatag atgttttagt agcaatgaat aaaccatctt tagattatat aaatttagtt
     5281 aaagaaggtg gaacagtagt tattaataca tctgcaatag atgagaatgt aacattaaga
     5341 gaagatataa gtgtagttaa aataaattgt caggaattag ctcaaaaagt aaacaatcca
     5401 aaagcagcaa atatagtagt acttggagca ttaattgcta aaactgggtt atttgaaaaa
     5461 gaattagctt taaaaactat gtgtgatttc tttgaggaaa aaggaaaagg aaagtttaat
     5521 gttcaaaatg aagcagcatt catggaaggc tataataacg ccatttgata aattaccctt
     5581 atcttaagtg atttatctca agatttaata atctttagtg ccaagagaaa cttcatcttg
     5641 agataaaatt cacttaaaat cacaactata aacatatagg agcgtgaatt tatgagtttg
     5701 gaaaaactta ttagaccaaa aagtattgct atagtaggag tgactgataa acttggtttt
     5761 ggaagaagtg cagcacttag tatagtaaaa agtaaggaaa cagatagagt atattatgtt
     5821 aatcccaaaa gagaagaatt gtttgggaga aaatgttata agacgataca agaagttcca
     5881 gaagtagtag actgtgtagt agtatgtacg cctagaaatg tagttccatc tgtattaaaa
     5941 gattctggag agttaggaac aaaggcagca gtggtatatg ctagtggatt tgcagaggaa
     6001 ggtacagagg aaggtacaga tttagaaaat caacttatag aaatatcaaa tacatacgat
     6061 atgaaaatat tgggtccaaa ctgcatgggg ttgctaaatt gtatagataa ggttaatcta
     6121 tgggcaggag gttctaaatg ggatttagat actaaaaaac ctggtatagg aattgtaggt
     6181 caaagtggat ttataactgc tgaaattgtt tcaagtgatt attttaatat atcttatggt
     6241 ttttcaacag gaaatggaaa tatagttaca ctagaagatg cagtagattt ccttgtagat
     6301 gatgattatg catcagtaat agcaatttac cttgaaggtt taaaaaatcc tcaaaaattt
     6361 atagatgctt taaaaagggc agcacaaaaa agaaagccag taataatatt gaaatcagga
     6421 aggtcagaaa aaggtgctat ttctgctgct tctcatacag gaaatttagc tggttcgagt
     6481 aaagcttttg aaagtatttt tgaaaaatat ggagtaattt ctgtggaaaa tttagaacag
     6541 tttatgtgtt tagcacaagc ttttagtgtt ttagatggta atttacctgc aaattctaat
     6601 tttgcagcaa taaacttctc aggaggagaa aatactattt gtgcagattt agcagaagaa
     6661 aatggtgtag aacttgcaga aatatcttct gaaactaaag aagaaatgga aaaatatctt
     6721 ccaggatttg caacacctaa aaatccacta gatgctacaa cagcattatt ccatgaaaag
     6781 gatatgatag taggtttatt acataccttt gaaaaggatg atagtgttgg atttactatg
     6841 gtaggtgcaa atataagaga tgaagaaaat gaaatgcatg aaaccctttg taaagctgta
     6901 agtgaagcta gagaacaagg attgaaaaaa cctgtgtttg cagttcctac acttgaagga
     6961 aatagatatt tagattatag gactagatta gaagataatc aagtgcctat aatgtcatca
     7021 gttggaacta catttacttg ttttaataaa atggctaagt ttatagatta tgattattca
     7081 aaaagaactt tagaatttaa agctgttaag aaaagagagt caaataatgt agtagcatta
     7141 tcagaattag atagtaagat tgagatgaaa aaatatggag taccagttcc aggacaaggt
     7201 aatgcaaaaa gtattgatga attagatgaa atcttaaaat atattaagta tcctgttgtt
     7261 ttaaaaataa attcttctga aatattgcac aaatctgatg ttggaggagt taaaatagga
     7321 attaagaata gagatgaggc agtagatgca tataatgaga ttttaactaa tgtaaaaaaa
     7381 gcaaaaccag atgctaatat tgatggtata ttagttcaag aaatggtaga aagtggtata
     7441 gaaattatta ttggtataac taatgatgac caatttggac caatgttatt agtcggttta
     7501 ggaggagtat ttgtagaggt atttaaagat actacattat atccattacc tataaatcat
     7561 gatgaagcta ttatgatgct taaaaaacta aaatcattta agcttttaaa tggctataga
     7621 ggaagtgagc catgtgatat tgatgctctt gcagatatga tggtcaagtt aggaaaatat
     7681 gcttatgaaa ataaggatga agtcaaggaa atagacttaa atccagtgtt tgtatatcct
     7741 aaaggaaaag gcgtatgtgc agtagatgcc ttaatagtta aatataaata gtttataagt
     7801 tttggtattc aaccataatt aaagcaccaa ctgaaaatca cattttgtaa gttagatagt
     7861 gtgattttca gtcaccccaa atttaattta aaaatatttt aaaatgaaag tatagtttta
     7921 agtttcattg tgaaatagca ctgtataaac aataaaatac tacgtataaa acgaataata
     7981 aaaggtgttt taatgataag aatcaatctt gattttcatt aaaacacctt ctttattata
     8041 ttaaaaattt ttaaacgtta tattgtcaca ggtgggtttg ttggagctgc atatgtacga
     8101 ttagcaaatt cttggtcaac aaagaatagc ccaccctctc cagcaagttt aacacgtttt
     8161 atattgtctt cacaatttgt ttcttcttct gcttgttcag atataaacca aagtaagaat
     8221 tgagaagttc taaagtctga ttcagagttt gctacagctg ctaagttgtt tattaaagct
     8281 gtaacctttt tttcatgagc taacgtacgc tcaagaatat ccattgcaga agtaaagtct
     8341 gaatctggca ttgggatagc atcaagctga acctttcctc ctacattatg taagtatttg
     8401 taaaaaaaca tagcgtggtc tgtctcttct ttatattgaa cataaaacca attagaaaat
     8461 cctttaagtc cttctgcttc aaggtaagaa gacatagaaa gataaagata cgcagaatat
     8521 aattcatggt taatctgctc attaagtaat ttttccattt tagctgaaat catttttata
     8581 cctccaaatt ttaaatgctc ttataaaata taataatatt aaatggaaaa atattcaata
     8641 aaagtgttta aatattttac atatttaata aaaatttata ctaccattaa aaaaattata
     8701 acttacaatg gctatagagc caattaatgg tgcatttgct ccaaaatttg aatgccttac
     8761 acttattttt ttatatttag aaaaacttgc tgaagcagtc aattttttta ataatgtatt
     8821 ttctataaat gtaccaggta tattaaagtc atagcctatt attatagaac tacaatcaat
     8881 caatgtaagt gtattaacta atgcatatga tacgtatgta caaaattcat caagcaaaaa
     8941 catggcaaga ctatctttat tatttgcctc atctattaac ttgactaaag aaaaatcttt
     9001 acagttaatt aatggggagt taggataaat atggctcaat gattcaattc ttttaattag
     9061 attactgaca cttgtataat aatctaaaca tccattgtta ccacaactgc acaatggtcc
     9121 actaaaatta attgatgtat gtcctatttc tccactttgt cctaggttgc cagtatgaag
     9181 tttattttct agaactagac cagctccaat accattcatt atgtgtaggt atatataatt
     9241 tgatatattt ttacctaaac catacatttt ttctgctaat gcacccgaat tagcatcatt
     9301 tatcaaaaat attggcaaat cagacatttc ttttataaaa tctactatat ttaggtttga
     9361 tacatttcca aaatctgggg gatttagtat ggtaccattt atatcatcta ctggacctat
     9421 tgaagaaata ccaattccaa ttatatctct cttgttgatt ttcattattt tagtaaacat
     9481 tctttttatt atatctttta aaacatcatt tgatagaaat ttagggtaag tttcagaaag
     9541 ttgttttaca atattacctt ttaaatctga aataattacc ttacatatat ttcttcttat
     9601 taggattcca caaatacaag gcgatatatt agaaatatct aataaaatag gtttacgacc
     9661 tgatgaatgt gcatttaaag ttgatgactc tatttcccta atcatatttt cattaataag
     9721 ttcagtaact agatttgata aagtcatttt agtaagtcct gtagacttag ctaaatcaac
     9781 tctggacata ggtgtatttg tagaaagtaa tttcaatatt attaacttat ttttaatttt
     9841 tacatctttg atgttagaac cttgtttgtt actcatcata aatctcctta aataaagtta
     9901 agtgttttat ttattttcaa gtatactatc accttataat agagtaacat ttttttactt
     9961 gaaaatatca ttaattagaa tatatcacat tatatatcat aaaacatata aaaaaatatt
    10021 agaattaaat taaaagtgaa aagtagattg atatataaat agtaaatact taattaaaaa
    10081 aagataatga tagcaataat tattgttacc aagaatctac tacttcaagt gttaccttag
    10141 gtatttaagt tgatagtggt ttgcacgtaa taaacttgat gtaatatata ttcttaataa
    10201 tatcgataaa agatttactt aaaaataata tagattatgt aaacatataa tctatttatt
    10261 gtatacaata aaatttcatt t
//
LOCUS       DAYAPA010000141        10058 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.145, whole genome shotgun sequence.
ACCESSION   DAYAPA010000141 DAYAPA010000000
VERSION     DAYAPA010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10058
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.145"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(170..640)
                     /locus_tag="ACGI59_003295"
     CDS             complement(170..640)
                     /locus_tag="ACGI59_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFG9741065.1"
                     /translation="MKYQVSLLAVKDIEVSKKFYKELFEQEIELDLGKNVTFKGGFAI
                     QEDFAWLTGINPDTIIQKSNNMELYFEVDDFDEFIEKLNYYKDVEFVHKPLKHEWQQR
                     VVRIYDPDKHIIEIGESMEVIARRYLSEGYSVEETSKIIQHPIKFVKQCENSKE"
     gene            complement(1374..1763)
                     /locus_tag="ACGI59_003296"
     CDS             complement(1374..1763)
                     /locus_tag="ACGI59_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HFG9741066.1"
                     /translation="MTIKKLPQSELKIMKFIWKSDSKVTSRDIVLGMEQKYQWKQTTT
                     LTLLSRLVVKRFLNSQKIDKYTHYEVLIKEKEYIGVETRDFFRNIHDSSIKSLLLSLH
                     ENINLSKDDILLIEEWIKNLKEEEKDV"
     gene            complement(3434..4840)
                     /locus_tag="ACGI59_003297"
     CDS             complement(3434..4840)
                     /locus_tag="ACGI59_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFG9741067.1"
                     /translation="MDSVKYILRRFIGSSILISLLFIVLNIAGFLLFSYMLFVSKYSD
                     NLDTSDSREIKSNLIMIEDNLILRDGKFILDDSSKEVLHKNKVWAMLIDNNGDRIWGF
                     DLPKEIPSHYTLTDVAKFSRFFLKDYPVYVWEHPNGILVTGYPKDKYTKFNLTYTVKE
                     IEQFPMLIIGMFLVNAIVTFLIALFIGLKMVKSIKPIITGIKLMSKGEPVLLQEKGIL
                     SDISKSINTVSKELQMKDEKLRKKEEARSNWIAGISHDIRTPLSMIIGYAGELEESSS
                     LSNREQEQVSIICNQGIKIRELVNDLNLVSKLEYNMQTLNCDKIRVSAFLRELISEFI
                     NNNLDNDFYVELDILNEDIIIDADKKLLKRAISNLIQNSITHNQQGCNIRVTSKSDLD
                     FCYIIVEDDGKGMSQERINSLLKSQNGLSEAYAKGQNHGLGILIVSGIINAHRGKLDI
                     TSIDSQGLKTILKLPLIK"
     gene            complement(4885..5592)
                     /locus_tag="ACGI59_003298"
     CDS             complement(4885..5592)
                     /locus_tag="ACGI59_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFG9741068.1"
                     /translation="MESIKDKKILVVDDHKELLKMIDEILRKEGFSRVFLASSYEEAV
                     RVFRNVKPDCAILDVVLPDGDGFSIMRKIRETSKIPVIFLSARGEDEDRLIGLGLGAD
                     DYIVKPFLPKELTLRLVGILNRVYVPIEEEELPVFKLGDSVVVNLNSACVEKDGQEIS
                     LTAKEHSLLLKLYENKGRIVTNDSLCQAIWGDDMYGYENTLMVHVRRVREKIEKTPST
                     PKHLITVRGLGYKLMIN"
     gene            5754..6677
                     /locus_tag="ACGI59_003299"
     CDS             5754..6677
                     /locus_tag="ACGI59_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9741069.1"
                     /translation="MKHMEMIIRTQRLSKEYNKIFRVKDIDLRVPQGAVYGFLGPNGA
                     GKSTTLKMLLGLAKPTQGNINILGKELNEKNRISILKDIGSLIESPSYYGHLTGLENM
                     TIMQRLLDLPKKNVNEALKIVRLENQKNKKVSQYSLGMKQRLGIAMAIMKFPKLLILD
                     EPTNGLDPAGIEEIRELIKSLPEKYGMTVLISSHLLSEIDQIATSIGIINHGELVFQD
                     SIKELHNRGQSQIAIKTKNVVSAQKLLNTKGYMSLIQDGYLTIENLKDSDVAKINGML
                     VHSNIDVFRIEEHKKSLEDIFLELTGKAVTL"
     gene            6677..7438
                     /locus_tag="ACGI59_003300"
     CDS             6677..7438
                     /locus_tag="ACGI59_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9741070.1"
                     /translation="MKYLGLEFYKLKRKKIILMTFLFAAVEIMWCIVNSNRAITRNPD
                     MLGGFEYSYMLMSFASLNGLFFPILISIITSRISDIEHKGDTWKLLKSSVTSLNSIYL
                     SKFLCSAILVSIPVLMQVLSIVLFGHFRGVIESISVSLLLKYTLGTILVSIAIIALHL
                     WIATVIPNQMIAITFGMIGSFIGLTSGLFYQGIRRLFIWSYYLELSPLSYAYDNVLGS
                     SVYRIDMNFSITILVFLIGMLIYYIGRNHLLKKEV"
     gene            7450..8187
                     /locus_tag="ACGI59_003301"
     CDS             7450..8187
                     /locus_tag="ACGI59_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9741071.1"
                     /translation="MLRKSVLGEILKLKNSFIWYVLLALPLISVLIGSGNFYLNQGIL
                     KKEWYSLWTQVSLFYGEFFFPILIAIFCAYICRLEHMNHNWNNVMTLPVNLKNIFLSK
                     LTVVSILTAITQVFFVIFYIVAGNMFGFSGSVPKELTGWIAKGWIISISIASIQLYMS
                     IKIRSFATPIGISLCLSLFGMGLIVAKPAVGIFFPYSMLGSAMGVIKQSALNVNDTIV
                     TICVAFITTIVFSEIANRNFRKNDVIS"
     gene            9065..9334
                     /locus_tag="ACGI59_003302"
     CDS             9065..9334
                     /locus_tag="ACGI59_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HFG9741072.1"
                     /translation="MFESRCGVCCDSCTKKEQVNCTGCPTMEKPFWGGECKVKTCCES
                     KELNHCGECDTFPCDMLLNKGKDQGFDPMVNIEQCRKWLEETVSN"
     gene            complement(9552..9797)
                     /gene="cdd1"
                     /locus_tag="ACGI59_003303"
     CDS             complement(9552..9797)
                     /gene="cdd1"
                     /locus_tag="ACGI59_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein Cdd1"
                     /protein_id="HFG9741073.1"
                     /translation="MGELQKIPGVGKATEKSLIMLGYTTIKSLKDANPAQMYEKECLM
                     RGQHIDRCQLYVYRCAVYFASTENPEPEKLKWWYWKD"
BASE COUNT         3412 a         1530 c         1092 g         4024 t
ORIGIN      
        1 taaatattca aatttattaa tatattttaa tattatattt attttttctt tattaatatt
       61 ttctttattt taatttaatt tatgacattt gtcaaatact agtcctatat attatcttga
      121 attagttcta ttacaattaa tttctactaa ataattataa tttaaacaat cattccttac
      181 tattttcaca ctgctttaca aatttaattg gatgttgaat tattttagaa gtttcttcta
      241 cagagtatcc ctcagataaa tatcttctag ctataacttc catagactct cctatctcaa
      301 taatatgctt atccgggtca taaatacgca ctactctttg ttgccattca tgttttagtg
      361 gcttatggac aaactctaca tctttataat aattgagttt ttcaataaat tcgtcaaaat
      421 catcaacctc aaaataaagt tccatattat ttgatttttg tataattgta tctggattaa
      481 tccctgtaag ccatgcgaag tcttcttgta tagcaaatcc tcctttaaat gttacatttt
      541 tgcctaagtc caattcaatt tcttgctcaa ataattcttt ataaaatttt tttgatactt
      601 caatatcctt cactgctaat aatgaaacct gatatttcat aaattttcct cctaattttt
      661 tatttctatt ttttcagtat cttctattaa catttttttc tctatctctt cagtaccttt
      721 tattaatcct ttatttttac ttttttagca tcttctatta acacttttgt ttctacctat
      781 tcagtatctt atatcaatct tttatttttt caatatcttc tattaacact tttatctcta
      841 tctattcagt actttctatc aatcttttgt ttttattttt tcaatatctt ctattaatac
      901 ttttgtttct acctcttcag tatcttatat caatctttta ttttttcaat atcttctgtt
      961 aacactttta tctctatctc tttaatatct tatattaatc ctttattttt attttttcaa
     1021 tatcatcatt aattcctatt tattttatca tcttaaatac gaaaacactg tgtcatattt
     1081 gcaaaatata tattatcatc tatattaaag cttcacaatt tatatttaaa aattctcttt
     1141 aattacacta cctctattta aaaagctacc ttaattatac ttataagtaa aggtagcttt
     1201 ttaaatttaa ttaaatagaa tatccactat taatttatat ttaatttatt tagattcaca
     1261 ttatagtatc ataatccact tataatcatt tgtcatatta cttaagtaga ctaatatatt
     1321 acataaatac acttacatac actttatatt gatgctataa gaaatacgtt tgcttatacg
     1381 tctttctcct cttcttttag attttttatc cactcttcaa taagtaaaat atcatctttg
     1441 ctcaaattaa tattttcatg taatgacaac aataaacttt ttatagaaga atcatgaata
     1501 tttctaaaaa aatctcttgt ctcaactcct atgtattcct tttctttaat taaaacttca
     1561 taatgcgtat atttatctat cttttgagaa tttagaaatc tttttactac taatctagat
     1621 aaaagagtca aagttgtcgt ttgtttccat tgatattttt gctccattcc taaaacaata
     1681 tctctagatg ttactttgga atctgatttc catataaatt tcattatctt taattctgat
     1741 tgtggcaact tttttatagt catgataaaa ccttgctact cagaatatag acaagcaata
     1801 tccataataa aatttttcga ttaaatacat cttatacgga tttatataag ctcagactta
     1861 taagatattt ttcaatctta atcctaaatt tagctatttg tacacggtaa atatatataa
     1921 ataatgtcaa aattctcttg tattatattg cttaatttta tgaaatattt tgtactcagc
     1981 tatctatttt ggatacttgt ccagtagctc tcctttcttt taaactcttt gattttaaat
     2041 atttgtattt cagtaaatcc tacttcctaa attttttatt tactacaact tattactgaa
     2101 cttcctactt cctaaacttt ttatttacta caacttatta ctgaacttct cacttactaa
     2161 atttcttact tactataaat tataactaaa cttcctactt tctaaatttc ttacttgcta
     2221 caaattatta ctaaacttcc tctccctaaa tttcttactt gctataaatt attactaaac
     2281 ttcctacttt ctaaatttct tacttactat aaattattac taaacttcct actccctaat
     2341 tttcttactt actataaatt attactaaat ttcctacttt ctaaatttct tacttactac
     2401 aaattattac taaacttcct actccctaaa tttcttactt gctataaatt attactaaac
     2461 ttcctactcc ctaagtttct tacttgctac aaattattac taaacttcct acttcctaaa
     2521 tttcttactt gctataaatt attactacta aacttcccac ttactaaatt tcttacttac
     2581 tactacttat aactaaactt cccacttcct aaatttctta cttactacca cttataacta
     2641 aacttcccac ttcctaaatt tcttacttac taccacttat cactaaactt cctactttct
     2701 aaatttcttg cttcttaaga ataatgttaa ttttaatttt aagattatag tttttacctt
     2761 gtttaattga aacattctat ataattatat ccttctattt tagagttttt ttatttatat
     2821 attacaaagt ttaaattttt tcattaatta tattataaat tttccatata tttcaacata
     2881 aatacatata tatatatgtt atttctaaaa atactacaca aaaactctac tttagtagaa
     2941 ctcaatggcg caaaatactt caatttaaaa catttatcgt actttaaata aataactata
     3001 ataaaatata cttacaatag ttacatagaa gctattataa ctacacagaa cagcttataa
     3061 ttaaaacttt tttaggatgt cattatattt tttcttaaaa ttaaaaaaca ctataccata
     3121 attctatttt agtagaattt ttttgtcaat aataaaatta aatattaact aaaaaataaa
     3181 aaaaatggaa ttatctaaag aatttaataa ccttacaata attccaaaat attatttcta
     3241 tgaaaatttt gataccatga gtaactcagc cataagcgca taaagcacaa gttactcttt
     3301 actttaataa aacttgacaa gaaaagaatt tatccttatt ctactctaat agagttaatt
     3361 tgtcaataaa gaataaaatt attttcaaaa tattttcatt cttcattctt atctgttatt
     3421 attttataaa atatcactta attagaggta attttagtat tgtttttagt ccctgactat
     3481 caatgctcgt aatatctaat ttacctctat gtgcattaat aatgccagat actatcaaaa
     3541 tacctagacc atgattttga cctttggcat atgcctcact tagtccattt tggctcttta
     3601 ataaactatt aattctttct tgactcatac cttttccatc atcttcaaca attatatagc
     3661 aaaaatctaa atcagactta gaggtaaccc ttatattaca tccttgttgg ttatgagtta
     3721 tactattttg tattaaatta ctaatagccc tttttaataa ttttttatct gcatctataa
     3781 taatatcttc atttagtata tcaagttcaa cgtaaaagtc attatccaaa ttattattta
     3841 taaactcaga aataagctct ctcaaaaacg cagaaactct aatcttatca caattcaatg
     3901 tttgcatatt atattcaagt ttagacacta aatttaggtc atttactaat tctctgattt
     3961 taatcccttg attgcatata atagacactt gctcttgttc tctatttgaa aggctactgc
     4021 tttcttcaag ttctccagca tatccaatta tcattgaaag tggagttctt atatcatgag
     4081 atattcctgc aatccagtta gacctagctt cctctttctt cctaagtttt tcatctttca
     4141 tttgcaactc tttagataca gtattaatag attttgaaat atcacttagt attccttttt
     4201 cttgtaaaag tactggttct ccttttgaca taagctttat gccagtaata attggtttta
     4261 tagatttcac catttttaaa cctatgaata gagctattaa aaaagtaact attgcattaa
     4321 ccagaaacat acctattatt aacataggaa attgttctat ttccttaacc gtataagtaa
     4381 ggttaaactt agtatacttg tcttttggat aacctgttac taatattcca tttggatgtt
     4441 cccatacata cacaggatag tcctttaaaa agaaccttga aaactttgct acatctgtca
     4501 aagtataatg acttggaatt tcttttggta aatcaaatcc ccatattcta tctccattgt
     4561 tatcaattaa cattgcccaa actttatttt tatgtagtac ttccttacta gaatcatcta
     4621 aaataaactt tccatctctt aaaatcaaat tatcttcaat cataatcaag tttgatttta
     4681 tttctctact atcacttgta tctaaattat cagagtattt agatacaaat aacatatatg
     4741 aaaacagaag aaaacctgct atatttaata caataaaaag taatgaaatc aagatacttg
     4801 aacctataaa tcgcctcaat atgtacttta cactatccaa aattaaatca ctcctcttga
     4861 tttcttttat tctaatatct aaaattaatt aatcatcaac ttatatccaa gacctcttac
     4921 tgtaataaga tgtttaggtg tagatggagt tttttctatc ttctctctaa cccttctaac
     4981 atgaaccata agggtatttt catatccata catatcatct ccccaaatgg cttgacatag
     5041 tgaatcatta gtaacaattc tccctttatt ttcatacaac tttaaaagca acgaatgctc
     5101 tttagctgtt aaagaaatct cttgcccatc tttctcaaca catgcactat ttaaattcac
     5161 aacaacagaa tctcccagtt taaatactgg caactcctct tcttctatag gcacatatac
     5221 acgatttaat atacccacaa gccttaaagt aagttccttt ggtaaaaatg gctttactat
     5281 ataatcgtca gcccctaagc ccaaaccaat caatctatct tcatcttcac ctcttgcaga
     5341 gagaaaaatc actggtattt tagatgtctc tctaattttt ctcataatag aaaaaccatc
     5401 tccatctggc aatacaacat ctaaaattgc acaatctggc tttacatttc taaataccct
     5461 aacagcttcc tcataacttg atgctaaaaa tactctacta aatccctctt ttctcaggat
     5521 ttcatctatc atttttaata attccttgtg gtcatcaacc acaagtattt ttttatcttt
     5581 tattgactcc ataatcatca ccctccttta gtataccata tacccatttt cacaactttg
     5641 cctaaataga cttttaaggt tattgtaagg taaatttcag ctaattgaaa tgtaaccaag
     5701 ttattatact gatataagtt aaaagaatca ataagcacag attaaaaaag gggatgaaac
     5761 atatggaaat gataattaga acacaaagac tttcaaagga atacaataaa atatttagag
     5821 taaaggatat agacctaaga gttccacaag gagctgtata tggttttttg ggtcctaatg
     5881 gagctggaaa atctaccact cttaaaatgc ttcttggact tgctaaaccc acacaaggaa
     5941 atattaatat tttaggtaaa gagttaaatg aaaaaaatag aatatcaatt ttaaaagata
     6001 taggttcttt gatagaatct ccatcatact atggtcatct tacagggctt gaaaacatga
     6061 ccataatgca aagactctta gatttaccta aaaaaaatgt aaatgaagca ttaaaaattg
     6121 taaggttaga aaatcaaaaa aataaaaaag ttagtcaata ttctttaggt atgaagcaac
     6181 gtcttggcat tgctatggca ataatgaagt ttcccaaatt attaatactt gatgaaccta
     6241 caaatggact cgacccagca gggattgagg aaattagaga attaataaaa tctctaccag
     6301 aaaaatacgg gatgactgtt ctaatctcta gccatctttt aagtgaaatt gaccaaattg
     6361 ccacatcaat aggaataata aatcatggcg aactggtttt tcaagatagc ataaaagaac
     6421 ttcataatag aggtcaaagt cagattgcta tcaaaactaa gaatgttgta tctgcacaaa
     6481 aactattaaa tactaaagga tatatgtcac ttatacaaga tgggtacttg actattgaaa
     6541 atttaaaaga ctcagatgtc gctaaaataa atggtatgct tgtccattca aatatagatg
     6601 tatttagaat agaagaacat aaaaaaagct tggaagatat attcttagaa cttactggta
     6661 aggcggtgac tttataatga aataccttgg tctagaattt tataaactta aaagaaagaa
     6721 gattattctg atgacctttt tatttgcagc tgttgaaata atgtggtgta tcgtaaattc
     6781 aaatagagct attacacgta atccagatat gttaggtgga tttgaatatt cctatatgct
     6841 tatgagtttt gcaagtttaa atggtctatt ctttcctatc ttaatatcaa taattacatc
     6901 acgtataagc gatatagaac ataaaggaga tacatggaag cttttaaaat cttctgtgac
     6961 atctttaaat agtatttatt tatctaaatt tttatgttct gctattttgg tatctatacc
     7021 tgtattgatg caagtgttat ctattgtgtt gtttggacat tttagagggg ttattgagtc
     7081 aatttctgta tctttacttt taaaatacac attaggaaca attcttgtaa gtattgctat
     7141 cattgcacta cacctatgga ttgcaactgt aattccaaat caaatgattg caattacatt
     7201 tggcatgatt ggtagtttta taggtttgac atcaggtttg ttttatcaag gtattcgtag
     7261 attgtttata tggtcttatt acctagagtt atcaccttta agttatgctt atgataatgt
     7321 tttaggttca tctgtctata gaattgatat gaatttttct attacgattt tagttttctt
     7381 aattggaatg ctaatttatt atattggaag aaatcattta ttaaaaaaag aagtatagga
     7441 gggatttaaa tgttaaggaa gtcagttttg ggagaaattc taaagttaaa aaattctttt
     7501 atatggtatg tattattagc tttgccactt ataagtgtgt tgattggttc tggtaatttt
     7561 tatctaaatc aaggcatact caaaaaagaa tggtacagct tatggactca agttagtctt
     7621 ttttatgggg aatttttctt tcctatctta atcgcaatct tttgtgcata tatttgtagg
     7681 cttgaacata tgaatcataa ttggaataat gtaatgacac tgccagttaa tttaaaaaat
     7741 atctttttat ctaaacttac agtagtttca atacttacag ctattacaca agttttcttt
     7801 gttatctttt atatagttgc aggaaatatg tttggttttt ctggtagtgt acctaaagaa
     7861 ttgactggtt ggattgctaa aggttggatt atttctattt caatagcttc tatacaatta
     7921 tacatgtcta taaagattag aagttttgca actcctattg gtattagtct ttgcctttct
     7981 ctatttggta tgggattaat agtagcaaaa cctgctgttg gtatattttt cccttattct
     8041 atgttaggta gtgctatggg ggttataaag caatctgctt taaatgttaa tgacactata
     8101 gttacaatat gtgttgcatt tattactact atagtatttt ctgaaattgc caatagaaat
     8161 tttagaaaga atgatgttat ttcttaatgg taaatgctag atatttttgg gtttgaaatt
     8221 tatattcttt ttagttgaaa tctatatcca ttttatttaa aaatatatat cttttatttg
     8281 aattttatat gttttatcta ctcgctatat aatagatata ctcctaaaat ttaaaaatat
     8341 ataataatgt tattatttaa aattattatg tattatatta aaacttaatg caaaaacaat
     8401 ttttatatta tataatgaaa cttaattaaa tataggtaaa aaatcattca atatattaaa
     8461 taaatattta actttaataa tctatattta attatagcta taacatagaa ttatcaaata
     8521 tattgaatga atatttgact cttggaatgt gtaattaaat taagtttggt tattcgatgg
     8581 aaggcaaatt tgatataaaa ctccatttaa agtgccagat aaaaacttta tggttatata
     8641 aaagttaaaa gtaatttata gtgttataga acattcaaag ggatttaaag taatctatgg
     8701 tgttttgaaa tattcaaagg gatttaaact aatctatagt cttgtagaat attcaaagag
     8761 atttaaaaca atctataatg ttgtaaaata tattcaaagg gatttaaact aatctatagt
     8821 cttgtagaat attcaaagag atttaaagca atctataatg ctgtaaaata tattcaaggg
     8881 gatttgaagt aatttattgt gttttgaaat attcaaaaag atttttggaa tagtttttat
     8941 aaatttacca gtactaataa ttttatgttt aacatttaga tgacctaaaa tcaactattt
     9001 gcacttttgt attcatctaa aatcatttta taatattttt aaataatgga aaggatgttt
     9061 gaatatgttt gaatcaagat gtggtgtctg ttgtgacagt tgtacaaaaa aagaacaagt
     9121 aaattgcact ggttgcccaa ctatggaaaa accattttgg ggtggagaat gtaaggttaa
     9181 aacttgctgt gaaagtaagg aacttaatca ttgtggtgaa tgtgatactt tcccttgtga
     9241 tatgctttta aataagggaa aagaccaagg ttttgaccct atggtaaata ttgagcaatg
     9301 tagaaaatgg cttgaagaaa cagtttctaa ttaattgttt tagaatgctt tgttaaggaa
     9361 tttatttaat cctttgttag gtagtatgtt tagtcatttg ttgattagta tatttataca
     9421 tttattgatg ggtatattta gccatacaaa tagatttaca taaaatttac gactaaaaaa
     9481 gagttgagta ttagttttta ttatttaaaa cggatattca actctttttt atgtctaact
     9541 tttttgattt tttaatcttt ccaataccac cactttaatt tttctggttc tggattttca
     9601 gttgatgcaa aatatactgc acatctgtat acatataact gacatctgtc tatgtgctgt
     9661 ccacgcatta aacattcctt ttcatacatt tgtgctgggt ttgcatcttt aagtgattta
     9721 atagttgtat atcctaacat gattaaagat ttttctgttg ctttccctac ccctggaatt
     9781 ttttgtagtt ctcccatact tcaccttctt tctggtatat tctttttata ttatacctat
     9841 taccagatat ttttttatta tagttaatat ttaatattta attttttatt atatcaattt
     9901 atttatgctc tttcatatat atatatttta ccacctctaa atcactgagt cacttaatta
     9961 catcataata tagttttata caaataaaat actttatgtt taatttaata tataaaattc
    10021 accttcaaga aaattatatt ataatctgac atttttat
//
LOCUS       DAYAPA010000142         9749 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.146, whole genome shotgun sequence.
ACCESSION   DAYAPA010000142 DAYAPA010000000
VERSION     DAYAPA010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9749
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.146"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            541..1449
                     /gene="trxB"
                     /locus_tag="ACGI59_003304"
     CDS             541..1449
                     /gene="trxB"
                     /locus_tag="ACGI59_003304"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFG9741074.1"
                     /translation="MVDIIVIGAGPAGLTSAIYAMRAGLSVTVFEKNIYGGQVASTSE
                     VENYPAVQKISGVEFSNNIYNQAVAQGVDIQFDEVEEINLEGKVKVVKTSSGEHKAKA
                     VILANGVERRKLGCAGEQEFTGRGVSYCATCDGAFFKDKEVAIVGGGNTALEDALFLA
                     NNCTKVYLIHRRDSFRGEEVLEKSVKARENIEILYSHGVEKIEGEKTVSKIEVKNLKT
                     EEKRTIDVSGIFIAIGLKPNNKMFENVLDLDEGGYIISDESCITSVEGVYVAGDSRTK
                     FLRQIITAASDGAIAAVQAANYINVE"
     gene            complement(1539..3362)
                     /gene="typA"
                     /locus_tag="ACGI59_003305"
     CDS             complement(1539..3362)
                     /gene="typA"
                     /locus_tag="ACGI59_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HFG9741075.1"
                     /translation="MSQKHKIINIAVIAHVDAGKSTLVDAFLSQSGVFRKNEVVKDCV
                     MDSNDLEKERGITIYSKNCAINYEDYKINIVDTPGHSDFSSEVERVMKTVDTVILLVD
                     ASEGPMPQTRFVLQKSLEFGLKPILFINKIDKKDQRAEEVVNEVFDLFVDLNATDEQC
                     EFPIIYGIAKQGIAKLEMDDDSEDLSPLFKTIVNHVEAYPNYDNEPLQFQISALAYDD
                     YVGRLGIGRIYKGTLKNNTQVAICREDSVVSKGKVSKLSVYEGLKQVEVDEATSGEIV
                     VIAGIPDISIGETICDLDSPLPMEMIKIEEPTLSMNFLVNDSPFVGKSGKFVTTRHLK
                     DRLEKELEVNVGLKVEPLDTTDGYKVSGRGELHLSILLENMRREGYEVGVSKPEVLMH
                     KEDGKLMEPIERVVVNCPEVYSGTIINELNMRKGMMESMSIEGDYVKIEFLAPTRGLL
                     GYRSEFINATRGEGTLVRSFEKFEEFKGEIPSRGNGVLIAQGPGVTMGYSLNALSDRA
                     VMFVDPGVEVYEGMIIGMNSRKDDMVVNPCKNKKMSNVRASGSDDAIKLSPPRIFTLE
                     EALEFIEDDELVEITPDSIRLRKRFLNEHDRLRYNKSRQGK"
     gene            3574..3816
                     /locus_tag="ACGI59_003306"
     CDS             3574..3816
                     /locus_tag="ACGI59_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9741076.1"
                     /translation="MLEVTKIIIKYFASESDTFVKEEVCNFEELLKWSHKFIEGKKIE
                     DVLYIKSKENYKVNVIKSLEIKELEIVRKQQIPPYM"
     gene            3964..4155
                     /locus_tag="ACGI59_003307"
     CDS             3964..4155
                     /locus_tag="ACGI59_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1450 domain-containing protein"
                     /protein_id="HFG9741077.1"
                     /translation="MIRVCPNCSNVDVNKLKTLVGEENVKTGCIGQCRAFKKEAVGII
                     DNELEIKETEAEFFEACKE"
     gene            4439..6940
                     /locus_tag="ACGI59_003308"
     CDS             4439..6940
                     /locus_tag="ACGI59_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFG9741078.1"
                     /translation="MSSNKVVESYKITGMTCAACAKAVERVTKKMDGVYDQSVNIATE
                     KLKIEYDNSKVNFDDIKQVVEKAGYGIIKEESNKKIDMKIDGMTCAACAKAVERVVKK
                     LDGVESISVNIATDKANIDYDPSKVKLSQIKAAIEKAGYKPIEEVKNKVDVDEDKLRK
                     EREMKSLFVKFIVAIVFAVPLFYIAMGPMIIKPIGPWPLPEIINPMTNTFNYALIQLI
                     LVIPVMIAGYKFYINGFKSLFSLSPNMDSLVAIGTLAAFLYSLYTTLQIANGQIQGMH
                     HHQLYYESAGIIIALILLGKYLESKSKGKTSEAIKKLMGLQPKTAIVLVDGKEVETPI
                     EEVEIGDILLVKPGTKIPVDGVVIEGYTSVDESMLTGESIPVEKNVGSKVTGASINKN
                     GVIKFKAEKIGGDTALAQIIKLVEDAQGTKAPIAKLADTVSGYFVPIVIAIAVVASLL
                     WFLIGGKDIVFVLTIFISVLVIACPCALGLATPTAIMVGTGKGAENGILIKGGEALES
                     AHKVNTVIFDKTGTITEGKPKVTDIVLNNNVKEEYLIKIASSAEKGSEHPLGEAIVKY
                     GEEKNIKFEKVDNFKAIPGAGIQVTINDESILLGNRKLMNDNNIKLGDLEEKSNILAS
                     QGKTPMYIAVDGNLSGIIAVADVVKESSKKAIEILHDMGIKVAMVTGDNAKTANAIAN
                     QVGIDMVLAEVLPEDKSKEVEKLQNQGKFVAMVGDGINDAPALAKADIGIAIGSGTDV
                     AIESADIVLMKSDLIDVPTAIKLSHETIKNIKQNLFWAFGYNTIGIPVAAGILYVFGG
                     PLLNPMIAAAAMSLSSVSVVSNALRLKNFKAYKRD"
     gene            7100..7768
                     /locus_tag="ACGI59_003309"
     CDS             7100..7768
                     /locus_tag="ACGI59_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFG9741079.1"
                     /translation="MNSSILVIEDDSNIQELISEFLSAEGYQVDTANDGLEGIQKFKQ
                     GSYDLVILDIMMPNLDGYGVCKMIRKSSSVPIIFLTALNDEGDQLKGFDLECDDYITK
                     PFSFNLLIKRVEAILRRSNKTINDKFIVFEKLKLDLNTYIAEIDGEPIELTLKEFNIL
                     KALIEKYPQVITREGLLDSIWGYDYYGDTRIVDAHIKNIRKKISLPYIKTVKGIGYTL
                     EKDI"
     gene            7752..9029
                     /locus_tag="ACGI59_003310"
     CDS             7752..9029
                     /locus_tag="ACGI59_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFG9741080.1"
                     /translation="MRRIFDKWEKLSIKYKLFSITTSLLIALALIIYLILYFLLPSYY
                     HEYKIESLQESLKSLVDSSIHFDTYTLEERLYYMAKDQNLAILLKDNQGKIVYGKNEV
                     VILRYSKYMINSLEDEYRTSIPIYTKDAKDGPYTLELVMPLQPIDEANEVIRKLMPYI
                     ISIAILIAIIGAYIYSIVITKPLINIIESEREQEYRRKDFVATISHELKTPITIISGQ
                     IEGMIYSVGKYKDRDTYLKKSYECTQELKDLVNEMIEVSKSEILEKDLKLVSINISEL
                     LNRLVKRQVFLIEEKHMKTILKIEENLEIKADQERITKAINNIINNAIKYSPEESELI
                     IRLYDKNKRISKKTSNQRVVLEIENTGVTIEKRYLEEIFNPFYRIEKSRSRKTGGSGL
                     GLYIVSQIFKSHGFDYSIKNKENSVVFTVEFKN"
     gene            9430..9651
                     /locus_tag="ACGI59_003311"
     CDS             9430..9651
                     /locus_tag="ACGI59_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9741081.1"
                     /translation="MDRLKRDMKNMKEDLKEGVKKTAYVMKNTAEDLKDDAQGAMVNL
                     EMKKDEMMERYEQKKFSKELEKQMKNEMK"
BASE COUNT         3880 a         1104 c         1647 g         3118 t
ORIGIN      
        1 agtttacaaa actttaattt gaaaaatgta attatatggt atatcatata aatataagta
       61 aatagtgcta atatttaaac tattaatcat aattggttca acaaaagtat atagttaata
      121 atgtttaatt tgaagaaatt gttagtgtga aattacatat atattcaaat taaattcata
      181 aatatttcac ataataatag tatactgtaa acaaaaggag gtattaaata tgggaatagt
      241 acttataaca gcagcattag ttttggttat gtatggactt tcaattctat catatatatc
      301 agaaagcaat atattagaag acactgtaaa tgataaccaa taaggttttg aattatataa
      361 ataagtgaga actgtaaata aaagttaatt tttatttgca gtttttttat atatatgtaa
      421 aataaagtta attaataaaa aataatgttt tgtttatagt atatatggat aataatagta
      481 aagtataaga atgatgagaa taatatataa gaaataataa tataaagaga ggtaaacgtt
      541 atggtagata tcattgtaat tggtgctgga ccagcaggtt taacttcagc aatttatgct
      601 atgagagcag ggttaagcgt tacagtattt gaaaaaaata tttatggagg tcaagttgca
      661 agtacctctg aggtagaaaa ttatccagca gttcaaaaga tttctggggt agaattttct
      721 aataatatat acaatcaagc agttgctcaa ggtgttgata ttcagtttga tgaggttgaa
      781 gagataaatt tagagggaaa agtaaaggtt gtaaaaactt cttcaggtga acataaagca
      841 aaggctgtta ttcttgcaaa tggagttgaa agacgtaaat taggttgtgc aggggaacaa
      901 gaatttacag gtagaggagt atcttactgt gcaacatgtg atggagcctt ttttaaagat
      961 aaagaagttg ctatagtagg tggaggaaat actgcactag aagatgcttt atttttagct
     1021 aataattgta caaaagtgta tttaattcac agaagagata gttttagagg tgaagaagtg
     1081 ttggagaaat ctgtgaaagc aagagagaat attgagatac tttacagtca tggtgtagaa
     1141 aaaatagaag gagaaaagac tgtatcgaaa atagaagtaa aaaacttaaa gactgaagaa
     1201 aaaagaacta tagatgttag tggtattttt attgctatag gtcttaaacc taataataaa
     1261 atgtttgaaa atgttcttga tttggatgaa ggtggatata taatttcaga tgagtcttgt
     1321 ataacatcag tagaaggtgt atatgtagca ggagatagta gaactaagtt tttacgtcaa
     1381 ataataacag cagcatcaga tggagctatt gcagccgttc aagcagctaa ttatataaat
     1441 gttgaataat ataaatttca aataaaaata gccatataaa ctgaaataat ttttcagtac
     1501 tatatggcta ttttgttaat tagaggtaaa tgtacgtttt atttaccttg tctagatttg
     1561 ttatatctta atctatcatg ttcatttaag aatctttttc taagtcttat agaatcaggt
     1621 gtaatttcaa ctaattcatc atcttctata aactctagtg cttcttcaag agtaaatatt
     1681 ctaggaggag ataattttat agcatcatct gaaccagaag cacgtacgtt actcattttc
     1741 ttgtttttac aagggttgac taccatatca tcttttctgg aattcatacc aattatcata
     1801 ccttcgtaaa cttctacacc agggtctaca aacattacag ctctatcact taaggcatta
     1861 agagaatacc ccatagtaac accaggtcct tgtgctatta aaacaccatt tcctcttgaa
     1921 ggtatttctc ctttaaattc ttcaaatttt tcaaatgaac gaactaaagt accttctcca
     1981 cgagtagcgt ttataaattc acttcgatat cctaaaagac cacgagtagg tgctaagaac
     2041 tctattttta catagtctcc ttctattgac atagactcca tcataccttt tctcatgttt
     2101 aattcattta taatagtacc agagtaaact tcaggacagt ttacaacaac tctttctatt
     2161 ggttccatta attttccatc ttctttatgc attaaaactt caggtttaga aacaccaact
     2221 tcataacctt cacgtctcat attttcaagt agtatagata agtgaagctc tccacgtcca
     2281 gaaactttgt atccatcagt agtatcaaga ggttcaactt taagaccaac atttacttca
     2341 agttcctttt caagtctatc tttcaaatgt ctagttgtaa cgaattttcc actttttcca
     2401 acgaatggag agtcgtttac taagaagttc atagaaagag taggctcttc tatttttatc
     2461 atttccatag gtagtggact atctaaatcg catatagttt cacctataga tatatcagga
     2521 ataccagcta taacaactat ttctccacta gtagcttcat caacttcaac ttgttttaaa
     2581 ccttcataca cagaaagttt tgatactttt cctttagata caacagaatc ttctctacat
     2641 atagcaactt gtgtattgtt tttaagagta cctttgtaaa ttctacctat accaagtcta
     2701 cctacataat catcatatgc aagagctgat atttggaact gtagaggttc attatcgtaa
     2761 ttaggataag cttcaacgtg atttacaata gttttaaata gaggagaaag gtcttcacta
     2821 tcatcatcca tttcaagttt agcaatacct tgtttagcaa taccatatat tataggaaat
     2881 tcgcattgct catctgttgc atttaagtct acaaataaat cgaaaacttc attaactact
     2941 tcttccgctc tctggtcttt tttatcaatc ttgtttataa ataatattgg ttttaaaccg
     3001 aactctaaag atttttgtaa aacgaatcta gtttgaggca ttgggccttc actagcatca
     3061 actaatagaa taactgtgtc aacagttttc ataacacgtt caacttcaga agaaaagtca
     3121 ctgtgtcctg gagtatcaac tatgtttatt ttgtaatctt catagtttat tgcacagttt
     3181 tttgaatata tagttatgcc tctttctttt tcaaggtcgt tactgtccat aacgcagtct
     3241 ttaactactt cgttctttct aaaaacaccg ctttgagaaa ggaaagcatc aaccaatgtt
     3301 gattttccag cgtcaacgtg ggctataact gcgatattta taattttatg tttttgtgac
     3361 atttttgttg ctcccttcga ttaaaataac ttattaatta tagcacaata tattattagg
     3421 tacaaggagg aatatattaa attgtattaa tatagtttaa attgaataaa actgttagtt
     3481 ttgcatctga aaattatttt gtttaaaaaa attttttaga caaataataa ccttaataga
     3541 taaaaagatt aataaaagag taaaggatga tatatgttgg aagtaaccaa aataataatt
     3601 aaatattttg caagtgaaag tgatactttt gtaaaagaag aagtttgtaa ttttgaagaa
     3661 cttcttaagt ggtctcataa atttattgaa ggtaaaaaga tagaagatgt tttgtatata
     3721 aagtctaaag aaaactataa agtaaatgtg attaagtctt tagaaattaa ggaattggaa
     3781 atagtgagaa aacaacagat accaccgtac atgtagtatt aataatatat aaaattaata
     3841 tgtaatactc gcaataggat ataaatggta tatatggtaa taatttatat taaaaaaact
     3901 tttataaagt ttatatttgg gtataaaagt attaattaga cattaattag gaggaactta
     3961 aatatgataa gagtatgtcc aaactgttca aatgtggatg taaataagct aaaaactttg
     4021 gttggagaag aaaatgtaaa gactggttgt ataggtcagt gtagagcatt taaaaaagaa
     4081 gctgttggaa taatagacaa tgagcttgaa ataaaggaaa cagaagcaga gttttttgaa
     4141 gcatgtaaag aataaatttt aaataaaata taataaatag agcatctaaa ttgtataagc
     4201 attaaacaat ttagatgctc tatttttata tatacattta attatgagta tacttttcta
     4261 ttaaatacaa ataaatataa ctaatacatt tatttattaa aaaaatattg atttaatata
     4321 cctatagggg gtataataaa aatatagatt tctaaataag taatttatca atattacata
     4381 tcaaaataaa acgttacaaa ttagctgaaa atatattgaa agaaaggaag attttattat
     4441 gagttcaaat aaagtggtag aaagttataa aattacaggt atgacatgtg cagcttgtgc
     4501 aaaggcagta gagagagtaa ctaagaagat ggatggagta tatgaccaaa gtgtaaatat
     4561 agctactgaa aaacttaaaa tagaatatga taattctaag gttaattttg atgatataaa
     4621 gcaggttgta gaaaaagcag gatatggaat tataaaggaa gaaagtaata aaaaaattga
     4681 tatgaaaatt gatggaatga catgtgcagc ttgtgcaaaa gctgtagaga gagtggtaaa
     4741 aaaattggat ggagtagaaa gtattagtgt gaatattgca acagataaag ctaatataga
     4801 ctatgaccca tcaaaagtaa aattatctca aataaaagca gctattgaaa aagctggata
     4861 taaaccaata gaagaggtaa aaaataaggt tgatgtggat gaagataaat tgagaaaaga
     4921 aagagaaatg aagtctttat ttgttaaatt tatagttgca atagtatttg cagtacctct
     4981 gttttatata gcaatgggac ctatgataat aaagcctata gggccatggc ctttgccaga
     5041 aattataaat cctatgacaa atacttttaa ttatgcatta attcagttaa ttttagtaat
     5101 accagtaatg atagctggtt ataaatttta tataaatggg tttaaatcac ttttttcttt
     5161 aagccctaat atggattctt tagttgcaat aggaacatta gccgcatttt tatatagtct
     5221 atatacgaca ctacaaatag ccaatggaca aattcaaggt atgcaccatc atcagttgta
     5281 ttatgaaagt gcaggaataa taatagcact tatcttactt ggtaaatatt tagaatcaaa
     5341 atcaaaagga aaaacatcag aagcaattaa aaagcttatg gggctacaac ctaaaactgc
     5401 aatagtgtta gttgatggta aagaagttga aactcctata gaagaagtag agataggaga
     5461 tatactatta gtaaaaccag gaactaaaat acctgtggat ggagttgtaa tagaaggata
     5521 tacatcagtt gatgaatcaa tgcttacagg tgaaagtata ccagtagaaa agaatgttgg
     5581 aagtaaggtg acaggagcta gtattaataa aaatggtgtt attaagttta aggctgaaaa
     5641 aattggtgga gatactgctc ttgcccaaat aataaagtta gttgaagatg cacaaggtac
     5701 aaaagcacct attgctaaac ttgccgatac tgtatctgga tattttgtac caatagtaat
     5761 tgcaatagct gtagttgcat cattattgtg gtttttaata ggaggaaaag atatagtatt
     5821 tgtacttact atatttatat cagtacttgt aattgcttgt ccttgtgcat taggattagc
     5881 aacaccaact gctataatgg ttgggactgg aaagggcgca gaaaatggaa tcttgataaa
     5941 aggtggagag gcattagaat ctgcacataa agtaaataca gtgatatttg ataaaacagg
     6001 tactataaca gaaggaaagc caaaagtaac agatatagta ttaaataata atgtaaaaga
     6061 agaatatctt atcaagattg cttcaagtgc agaaaaaggt tcagaacatc ctcttggaga
     6121 agcaatagtt aagtatggag aagagaagaa tataaaattt gaaaaagttg ataattttaa
     6181 agccatacca ggtgctggta tacaagttac tattaatgat gaaagtatat tacttggaaa
     6241 tagaaagctt atgaatgaca ataatataaa gcttggggat ttagaagaaa aatcaaatat
     6301 attggcaagc caaggtaaaa cacctatgta tatagctgta gatggaaatt tatcaggtat
     6361 aatagcagtt gcagatgtag tcaaagaaag tagtaaaaaa gcaatagaaa tacttcatga
     6421 tatgggaata aaagttgcaa tggtaactgg agataatgcg aaaactgcaa atgctatagc
     6481 aaatcaagtc ggtatagata tggttttggc agaggtttta ccagaagata agtcaaaaga
     6541 agttgaaaaa ctacaaaatc aaggaaagtt tgttgcaatg gtaggggatg gaataaatga
     6601 tgccccagct cttgcaaaag cagatatagg gatagcaata ggaagtggaa cggatgtagc
     6661 cattgaatct gcggatatag tacttatgaa gagtgattta attgatgttc caacagcaat
     6721 taaacttagt catgaaacta taaaaaatat aaaacaaaac ctattttggg catttggata
     6781 taatacaatt ggtatacctg tagcagcagg aatattatat gtatttggag gacctttatt
     6841 aaacccaatg attgcagcag cagcaatgag cttaagttca gtttcagtcg tatcaaatgc
     6901 acttagactt aaaaacttca aggcatataa gagagattag attaataaat taaataaaat
     6961 gtatccaaaa gagctatctt taaattaaaa ggattaattt acaaattaag atagctcttt
     7021 tttcatgtaa aattcacaac aataagataa aatataagta atatacgaaa ttttaaataa
     7081 aatagaaagg attgatagaa tgaattcgtc aatacttgta attgaagatg attcaaatat
     7141 acaagagtta atatcagaat ttttaagtgc agaaggttat caagttgata cagcaaatga
     7201 tgggttggaa ggtatacaaa agtttaagca aggaagttat gatttagtaa tattagatat
     7261 aatgatgcca aacttagatg gttatggagt ttgtaaaatg ataagaaagt catcaagcgt
     7321 tccaattata tttttaactg ctttaaatga tgaaggagac caacttaaag ggtttgactt
     7381 agaatgtgat gattatataa caaaaccatt ttcatttaat cttcttataa aaagagtaga
     7441 agcaatttta aggcgaagta acaagactat aaatgacaaa tttatagttt ttgaaaaatt
     7501 aaaattagat ttaaacacat atattgcaga gatagatgga gaacctatag aacttacact
     7561 aaaagagttt aatatattaa aagctttgat agaaaagtat ccacaagtta taactaggga
     7621 aggtcttttg gatagtatat ggggttatga ctattatgga gatactagga ttgttgatgc
     7681 acatataaaa aatataagaa aaaaaatatc attaccatat attaaaactg taaaaggaat
     7741 aggatataca cttgagaagg atatttgata agtgggaaaa gttaagtata aagtataagt
     7801 tattttcaat aacgacatca ttattaatag cattagcttt aataatatat ctaattttat
     7861 attttttatt gccatcatat tatcatgagt ataaaattga gtctcttcaa gaaagtttga
     7921 aatctttagt agatagttca attcatttcg atacatatac tttagaagaa agactttatt
     7981 atatggctaa agaccaaaat ttggctattt tattaaaaga taaccaaggt aaaattgtat
     8041 atggtaagaa tgaagtagtt attttaagat atagtaagta catgattaat agtcttgagg
     8101 atgagtatag aacttctata cctatatata ctaaagatgc aaaagatggt ccatatactt
     8161 tagaactcgt tatgccatta caaccaattg acgaagcaaa cgaagttata agaaaactaa
     8221 tgccatacat catatcaata gcaatattga tagcgattat tggagcatat atatattcaa
     8281 ttgtaataac aaagcctctt ataaacataa tagagagtga gagagaacag gaatatagaa
     8341 ggaaggattt tgttgctaca atatcacatg aactaaaaac tccaataacc ataataagtg
     8401 gtcaaattga aggtatgatt tatagtgtag gtaagtataa ggatagggat acttatctga
     8461 aaaaatcgta tgagtgtaca caagaattaa aagatttagt aaatgaaatg atagaggttt
     8521 caaaatcaga aatacttgaa aaagatttga aattggtatc aataaatata agtgaattat
     8581 taaatagact tgtaaagaga caagtatttt taatagaaga aaaacatatg aaaactattt
     8641 taaaaataga agaaaatttg gaaattaaag cagaccaaga gagaataact aaggcaatca
     8701 ataatataat aaataatgcc ataaaatatt ctcctgaaga atcagagctt ataattagac
     8761 tctatgataa aaataaacga ataagtaaaa agacttctaa tcaaagagtg gttttagaaa
     8821 ttgaaaatac tggtgtaact atcgagaaac gttatttaga agaaatattt aatccatttt
     8881 atagaataga aaaatctcgt tcgagaaaaa ctggaggaag tggacttgga ctttatatag
     8941 tcagccaaat ttttaaaagt catgggtttg attatagcat aaaaaacaaa gaaaactctg
     9001 tagtattcac agtagaattt aaaaattaat tacttattat aaagaatata taactttcaa
     9061 taatagttca ttttaaaatc acaaaaaatt cataatgaaa ctgtattata taaatataaa
     9121 taagcttaaa accataaaat acaccaacta taatgatgag atttccttac atcctccata
     9181 taagtcatca ttatagtaac ccaacaatta agttactatg aatttaaaaa ataacaccga
     9241 gcatataatc tatgcttagt gttattttta ttttgaaata aaaaatattt aatttattga
     9301 aatgtataga tttagatagt ttaaactata tttaagactt aaaaattata attgatatta
     9361 caaataaata tatgtacttt gggaaaaata aatataggaa gtattatatt tacagaggag
     9421 gtagttttga tggatagatt aaaaagagat atgaaaaata tgaaagaaga tttaaaagaa
     9481 ggtgttaaaa agacagctta tgttatgaaa aatacagctg aagatttgaa agatgatgca
     9541 caaggtgcta tggtaaactt agaaatgaaa aaagatgaga tgatggaaag atatgaacaa
     9601 aagaaatttt ctaaagaatt agagaagcaa atgaagaatg aaatgaaata agcttttatt
     9661 tatgaataac aaattattgt attcatataa aaagtagcca gtaatattaa atagtttatt
     9721 taaggaaata gactttctaa aaataactg
//
LOCUS       DAYAPA010000143         9719 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.147, whole genome shotgun sequence.
ACCESSION   DAYAPA010000143 DAYAPA010000000
VERSION     DAYAPA010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9719
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.147"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            160..390
                     /locus_tag="ACGI59_003312"
     CDS             160..390
                     /locus_tag="ACGI59_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2922 domain-containing protein"
                     /protein_id="HFG9741082.1"
                     /translation="MEIKKKLVMSFKNVKDKQVSFSIENPKDDLTEENIKSVMDLIVS
                     KNIFSVGGFDLASVVEAKVVETNTTPYDLVIE"
     gene            403..552
                     /locus_tag="ACGI59_003313"
     CDS             403..552
                     /locus_tag="ACGI59_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvrJ family protein"
                     /protein_id="HFG9741083.1"
                     /translation="MDDSLQLLITNVGFPIALSMYLLVRIEGKLNTLSNSIDTLSKNI
                     LIFKK"
     gene            809..1246
                     /locus_tag="ACGI59_003314"
     CDS             809..1246
                     /locus_tag="ACGI59_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HFG9741084.1"
                     /translation="MKIHVEQGINCDDIEITIKCSALDNNIEKIISLLSIPLLDIKGK
                     KSGEIYKLNVCDILYFETVGNKTFAYYEDDVFEIDLKIYKLSKLLANTSFSRISKSMI
                     LNMDYIKSIKVISHNRMQATLKNNEKVIINRRYIKNIKEKLNI"
     gene            1503..2396
                     /locus_tag="ACGI59_003315"
     CDS             1503..2396
                     /locus_tag="ACGI59_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HFG9741085.1"
                     /translation="MQLYNTPFNTNKHLIYFFTFTLVLNWIFSLTPALLGITNTILGE
                     IIFYLAYTSPAIVGLFFISTIYPQSAKQDFIYRYLSFKEIGIKWILLTILFFALTFIT
                     SLIIGNYFNVKTSDIDWIHIAVFRPSKIAYMLLASFILSILSQEAGWRGYAIDKLLVR
                     FGFIGSSIILGLVCGIWYLGSYFTPNQIPYNLAQYSLFDAFLFIPNIVLLNFIINFVY
                     INTSRSILSAGLVHMMYYFFNIQLLLHYPIKLCVIAQYVQIIFGLIFLVYMISSNKFK
                     QKVNSEIEQIKSDDFEFELDW"
     gene            2574..3398
                     /locus_tag="ACGI59_003316"
     CDS             2574..3398
                     /locus_tag="ACGI59_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFG9741086.1"
                     /translation="MFKIGEVSKLTQISIRMLRYYDELGILKPAKTDKYTGHRLYSVE
                     QISILQRIVLLRDSKFSVAEIANIVHNWNDEFVIKELNRKKNEIQKEIKQEQQRINKI
                     DKFIEAINCDKDEIHYNVVFKKIPSYKIISLREIIPDYQSEGILWEKLSKFIKEEHIE
                     VSRQSNNNIAFYHDEEVKDNGVDIEVGMVVKKIGKNKSGFIYRETEEIDMMACTMVYG
                     PYENIAGAYESFCYWLDKNSDYQISGINRQIGHKGEHNEINPENYLTEIQIPLIKV"
     gene            3524..4105
                     /locus_tag="ACGI59_003317"
     CDS             3524..4105
                     /locus_tag="ACGI59_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFG9741087.1"
                     /translation="MKADSTKEQLINATIQLLSSHRDVSSITAREIVAKANVNLAMIN
                     YYFKSKEELINISIKKFLKRHMDEYKATHDTKNLAPKQQLREILIAFFDFIGEHSQFI
                     KISVPYILIQEEIVYPFEILPLIKAHYENQNNKKTEAEYRTIAYQITSFIQLLFLRSN
                     AFFKYSGIDILNIEQRNGLIDFQLDLLLVDITK"
     gene            4209..4385
                     /locus_tag="ACGI59_003318"
     CDS             4209..4385
                     /locus_tag="ACGI59_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9741088.1"
                     /translation="MFFTFIGVISAILLLCTIICGLWIKLNNRNNDSNSHFTFSILSI
                     AFSLLIVVAFLVRN"
     gene            5651..6403
                     /locus_tag="ACGI59_003319"
     CDS             5651..6403
                     /locus_tag="ACGI59_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9741089.1"
                     /translation="MLKVNQLCKSYKTGSKTYPVLKNISFEINKGEFVAIMGSSGSGK
                     TTLLNCISCFIPIDNGEIILGNNNIRNLKEKELSEVRNQQLGFVFQDFMLFDGLSVFE
                     NICVPQIIKGSQVLQMENRAINLCELFGIEHIRKKYPAEISGGEKQRTAVARALMNNP
                     DLILADEPTGNLDSKSCRTVIESFLKAKQELDATILMVTHDSFAASFCDRVIVLKDGI
                     IYKEVVKSNSRHKFLDELLEVLKEMGGAQDDN"
     gene            6393..8504
                     /locus_tag="ACGI59_003320"
     CDS             6393..8504
                     /locus_tag="ACGI59_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9741090.1"
                     /translation="MTINKLYSKLRAYNWKNYTTLFFCIILSVVLVTSYALIYFSPTI
                     QNILPTGGDSRKQATMIFSVAILGCAVFTTYASSLFLKFKSKEIGIFMALGTDKKKLK
                     KLLFSEILLISIVCCFLGLILSIPVSFGIWKIFQLVIIDTREMTYHFGTQGFIYGFIF
                     SVFVTLCIFILSVKFINRTNIIDILNDQRISEPIQDVKDWYGILGIIFIILGIFLGYL
                     VPSLTINKLNYKLPSIWSFTYLLSLIGIYMIMTYIVVHAKRGKHPEKYYKNIISTSMM
                     RFMGRQTVKNMCVISFLIAGALFAVFYVPNMVTGIYDTLKNNPIDYTFTYNQRDKQVS
                     KKDIEQLADKHNIKIQNYMEIPSIELIVDGVESIYHKDGTIEKVYMKKSSYDMFFNSK
                     DFSKLIGKPITIKNGEYLAVVNKGNENQDYKYTKFISSPVTEKEIPFRYAGKIIFNGS
                     FFQNRSMNAYVLNEEDYKSFTKDLSIKNYNNSILFNVDKDTYAFANDLKKEIIKRTSK
                     EMGMFSGYDEYVKKRYESTGRKYFMDEEYPSGEGHLKLDPNNNQLYLNWKYYPNFKIL
                     QSQDMIKNMAVFLMLFIYVAIICFTAVGIIAYTRGVTIAINHKQVFMDLNRLGANNDY
                     IKFCIRNQLKKVFSYPLIAGSFLIYLFNFMIMLNNDGRIVNSEVNALLINLGFIGICG
                     VYMYLIYLLTYKKFKKIIGIL"
     gene            8837..9547
                     /locus_tag="ACGI59_003321"
     CDS             8837..9547
                     /locus_tag="ACGI59_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFG9741091.1"
                     /translation="MTSICICDDEKQQRNMLNKIITREMDLWGYPYQIKEYSNGESLV
                     YNLEHENYYIDIIFLDIELGKSNGVDIAKTIRKHSKDTIIIFVTGFSDYVFHGYDVGA
                     LNYILKPYKTEKICKVLKEALKKLEQWKENFITVQIGTNLCKINTKDILYFKSELRKL
                     TVVTFDKTLEYYGKLNDMETVLPSNFIRTHQRYIVNLNHVDSITQTYAKVQNEKIPIS
                     RKKYQEVVGKFTRYMLES"
BASE COUNT         3720 a         1215 c         1280 g         3504 t
ORIGIN      
        1 taatattgac aattgaaata ttaatactaa caagcaaaac attaatattg acaattgaaa
       61 tattaatact aacaagcaaa acattaatat tgataattaa aataccaata ttaacaagca
      121 aacaattaaa atataaatta ttatatcgga ggtaattata tggaaattaa gaaaaaatta
      181 gtcatgagct ttaaaaatgt aaaggataaa caagtttctt tttctataga aaatccaaag
      241 gatgatttaa ctgaagaaaa tataaaatct gtcatggatt tgatagtatc taaaaatatt
      301 ttttcggttg gaggatttga cctagcttct gttgttgaag ccaaggttgt ggaaacaaat
      361 accactcctt atgacttagt tatagaatag ggggctaatt ttatggatga tagtttacaa
      421 ctgttaataa ccaatgttgg ttttcctata gctctatcca tgtacttgtt ggttagaatt
      481 gagggtaaat taaatacttt atcaaacagt attgatacat tatccaaaaa tattttgata
      541 tttaagaagt aatttgacaa tatatgtaag tcataaaata ggtctatttg taaaataatt
      601 agacatattc tatgtgaaat actaggatat ttggataatt tatccaaata tccatagtat
      661 ttttttgaat tttaccacct caaatttgaa tttcactgtt ttatttttga atttcactat
      721 gcttaatttg aaatttagaa cgcatctagt tggactcaat aagatataat gctattctaa
      781 tgacagtaat aaaatgaggt gatgattaat gaaaatacat gtcgaacaag gtattaattg
      841 tgatgatatt gaaataacca taaaatgttc tgctctagat aataacattg aaaaaataat
      901 ttcattattg agtataccgt tacttgatat aaaaggtaaa aaatcaggag agatttataa
      961 actcaatgtc tgtgatattt tatactttga gacagttgga aataaaacat ttgcttatta
     1021 tgaagatgat gtatttgaaa ttgatttaaa aatatataaa ttatcaaaat tattagctaa
     1081 tacatctttc tcacgaatat ctaaatccat gattttaaat atggactaca taaaaagtat
     1141 aaaagtaata tcacataatc gcatgcaagc tactttaaaa aacaacgaaa aagttattat
     1201 aaatcgtcgt tatataaaaa atattaaaga aaagctaaac atttaataaa aatctaaagt
     1261 aatttattaa tatcactaat tgcaaattta ttgacctatt taataattaa taataatata
     1321 atatattcaa tattaaatac taattaatta tatttagtgt aaaaataatt aatcaaaata
     1381 taaaaaatag gatattatta taggcaataa ctaacatcaa gcgataactt aatacatata
     1441 aaaataaata aacaattaat aaaaattaaa acttcattat aaaacggaag gatagtgaaa
     1501 aaatgcaact atataacacc ccctttaata caaacaaaca cttaatctat ttctttacat
     1561 ttactctagt attgaactgg atattcagtc ttacacctgc gttgctcgga attacaaata
     1621 caatacttgg agaaatcatt ttttatcttg catatacatc accagctatt gtaggtctat
     1681 tttttatttc tacaatctat ccacaaagtg caaaacaaga ttttatttat agatacctaa
     1741 gctttaaaga aatcggaata aaatggatac tattaacaat attatttttt gctttaactt
     1801 ttattacttc gttaataatc ggaaattact ttaatgtaaa aacttctgat atagattgga
     1861 tacacatagc agtgtttaga ccttctaaaa tagcttatat gctattagca agttttatat
     1921 tgagtatact tagtcaagaa gctggatgga gaggatatgc cattgacaaa ttattagtta
     1981 gatttggatt tataggctct tcaattattt tagggcttgt ttgtggaatc tggtatctag
     2041 ggtcgtattt tacacctaat caaataccat ataatttggc acaatactcc ctatttgatg
     2101 cttttttatt tataccaaac attgtacttt taaactttat aattaatttt gtatatatca
     2161 atacaagtag aagcatttta tcagctggat tagttcatat gatgtactat tttttcaaca
     2221 ttcaattact tctacactac cctattaaat tatgtgtaat agctcaatat gtacaaataa
     2281 tatttggttt aatatttctt gtatatatga tttcttctaa taaatttaaa cagaaggtaa
     2341 attctgaaat tgaacagata aagtcggatg attttgaatt tgagttagac tggtaatttt
     2401 aatatgaatt ataaattaag ataatctatg atttgatacg attactactt aaatataatt
     2461 tactctctat aatttattgc aacatctgga aaataattgt atttccacaa acgcttgaag
     2521 tatataaaaa caaaatatat actgattata agtgaaaagg aggtcatatt ttcatgttta
     2581 aaataggaga ggtttcaaaa ctaacacaaa tttctattcg tatgttgcgt tattatgatg
     2641 aacttggtat tttaaaacct gcaaaaacag acaaatatac aggtcataga ttatactcag
     2701 ttgaacagat ttcaatatta caaagaattg tattattgag agattcaaaa tttagcgttg
     2761 cagaaatagc aaatatcgta cataattgga atgatgaatt tgtaattaag gaattgaata
     2821 gaaaaaagaa tgaaatacaa aaggaaataa agcaggaaca acaaagaata aataaaattg
     2881 ataaatttat agaagctata aactgtgata aagatgaaat tcattacaat gttgtcttta
     2941 aaaagattcc aagttataaa atcatatcac ttagagagat aattccagac taccaatctg
     3001 aaggtatact ctgggaaaag ctatctaaat ttatcaaaga agaacatata gaagtatcac
     3061 gtcaatccaa caataacata gctttttacc atgatgaaga agtcaaagat aatggtgtag
     3121 atattgaagt tggaatggtt gtaaagaaga ttggaaagaa caaatctgga tttatatatc
     3181 gtgagacaga agaaattgat atgatggcat gtactatggt ttatggtcca tatgaaaata
     3241 tagctggtgc atatgaatcg ttctgttatt ggttagataa aaatagtgat tatcaaatat
     3301 caggtataaa tagacaaatc ggacataaag gtgaacataa tgaaataaat cctgaaaatt
     3361 atcttacaga aattcaaatc ccccttataa aagtctaata gattagtact taaatactta
     3421 agtactaatc acccattgac atcccaaaat tttcatgata gtatctaaac gtgtattaaa
     3481 catgtttaat ttttttatgg agaaactttt aaggggaaaa attatgaaag cagattcaac
     3541 aaaggaacaa ttaattaatg ccacaatcca gttactatct tcacatagag atgtttcaag
     3601 cattacggct agagaaattg tagctaaagc aaatgtcaat ttagctatga ttaattatta
     3661 ttttaaatct aaagaggagt taattaatat ttctattaaa aaatttttaa agcgtcatat
     3721 ggatgaatat aaggctacac atgatactaa aaatttagca cctaaacagc aattaaggga
     3781 aatactaatt gcattttttg attttatagg tgaacatagt caatttataa agatttctgt
     3841 accctatata ttgatacagg aagaaattgt ttatccattt gagatactac ctttaattaa
     3901 ggctcactat gaaaatcaga ataacaaaaa aactgaagct gaatacagaa ctattgcgta
     3961 tcaaataact tcatttattc agttgctttt tcttcgttct aatgcttttt tcaagtatag
     4021 tggtattgat attcttaata ttgaacagag aaatggttta atagattttc aattagattt
     4081 attattggta gatattacaa aataatacta tattaatttt ttatgtacat ttttttattt
     4141 tatggtaacg tttgaaaata ttctcattga ctaaaaatac ttgcattaaa taagggggag
     4201 gaattttaat gttttttaca tttataggag taatatcagc aattttattg ttgtgtacaa
     4261 ttatatgtgg tttatggata aaacttaata atcgtaataa tgactctaat tctcacttta
     4321 ctttttcaat actttcaatt gctttttcac tgttaattgt ggtggccttt ttagtaagaa
     4381 attaatgtct ttgttatgct gaggtttatt attatagatt tttattattg catgattttt
     4441 actatattat agtattttta ttatattaca gggtcttatt tattatagtg ttttgttgtt
     4501 acagcatttt attatattac agagtcctat ttattatagg atttttatta tattgtaaaa
     4561 tcttatttat tataggattt tgttatatta cagactttta ttatattgaa atctcttatt
     4621 tattgcagtg tttctattat attgcaaact cttatttatt atggtgtttt gttattgcaa
     4681 catttctatt atattgtaaa gtcttattta ttacagttct ttattatact acagaccttt
     4741 attattacag catttctatt acattgtaaa gtcttattta ttacagttct ttattacact
     4801 acagactttt attattacaa tctcttattt attacagttc tttgctatac tacagacctt
     4861 tattattaca gcatttctat tacattgcaa agtcttattt attacagttc tttattatac
     4921 tacagacttt tattattaca gcatttctat tacattgcaa agtcttattt attacagttc
     4981 tttattatac tacaaacctt tattattaca acattttatt attttaactt ctttactata
     5041 atatagtaat attttaatat ctcaaaatca tatcaatcac ttttcagcat agtctaaaat
     5101 acttccactt tctaaaatta tctatcatac ctatactaat aatacttttt aaccttttta
     5161 ttatacattt caattgtttc taacaaccta tttatttcca aaactccaat atattttctc
     5221 aattacattg tgttaatagc ccctaaatcc tttcaaatta tttacaatac attccattaa
     5281 ataatcttac tgctctataa ttcttttttt ctttttaacc ctactataca ttatttacat
     5341 catttttttc aatacaacat tcgttattct ataacctttt tacttttact atatacttct
     5401 tttctaatta caccttcaat gttccacatc atattacttt agcacaacaa ttaataccta
     5461 aaaataaccc tctaaatcct ccaataatat aatcaattta actataataa taacctctca
     5521 cactatccaa acaatatatt ttcaaacatt acaaaaaaac atccaactat tacaatatag
     5581 ctcatatccc tcctcccctt tcaaatgcta tcattgactt acaagataaa tgaaagtgag
     5641 gatgatatat atgcttaaag taaaccaatt atgtaaatca tataaaacag gcagtaaaac
     5701 atatcctgta ttaaaaaata tttcttttga aatcaacaaa ggagaatttg tcgcaatcat
     5761 gggttcatca ggaagtggaa aaactactct tttaaattgt atttcatgtt ttatcccaat
     5821 tgataatggg gaaataattc ttggaaataa taatataaga aatttaaaag aaaaggaact
     5881 ttcagaagtt agaaatcaac aactaggatt tgtattccaa gacttcatgc tatttgatgg
     5941 tcttagtgtc tttgaaaata tatgtgttcc acaaattatc aaaggtagtc aagtcttaca
     6001 aatggaaaac cgtgcaatta atctatgtga gctatttggt attgaacata tcagaaaaaa
     6061 ataccctgcc gaaatatctg gtggagaaaa acagcgtaca gcagtagcaa gagcactcat
     6121 gaacaaccca gatttaatat tagcagatga accaactggt aaccttgata gtaagtcttg
     6181 tcgtacagtc attgaatcat ttttaaaagc aaagcaagaa ttagatgcta ctatactcat
     6241 ggttacacac gatagctttg cagcaagctt ctgtgaccga gttattgtgc ttaaagatgg
     6301 aataatttat aaagaagttg taaaatcaaa ttcaagacat aaattcctag atgaattact
     6361 agaagtttta aaagaaatgg gaggcgctca agatgacaat taataaatta tacagtaaac
     6421 ttcgagccta taattggaag aactatacaa ctttattctt ttgtataata ctttctgtgg
     6481 tattagtaac ttcatatgct ctaatttatt ttagcccaac aatacaaaac atacttccaa
     6541 ctggtggaga tagcagaaaa caggctacaa tgatattctc agttgctata ttaggatgcg
     6601 ctgtctttac tacatacgct tcttccctct ttttaaaatt taaaagcaag gaaattggta
     6661 ttttcatggc tctaggaaca gataagaaga aattaaaaaa acttctattt tcagagatat
     6721 tgttaatttc aatagtatgc tgttttttag gtcttatatt aagtattcct gtatcttttg
     6781 gtatatggaa aatatttcaa ttagtgatta ttgataccag agaaatgaca tatcattttg
     6841 gaactcaagg attcatatat ggatttatat ttagtgtatt tgtgacatta tgcatattta
     6901 tactttctgt aaaatttatt aatcgtacaa atataattga tatattaaat gaccagagaa
     6961 tatctgaacc aatacaggat gtaaaggact ggtatggtat attaggtata atttttatta
     7021 tactcggtat ttttctagga tatctcgtac cttcactaac tataaacaaa ttaaattata
     7081 aactaccatc tatatggagc tttacttatc tactctctct tattggaata tatatgatta
     7141 tgacatacat agttgtacat gctaaaagag gaaaacatcc agagaaatac tataaaaaca
     7201 tcatatcaac tagcatgatg agatttatgg gaagacaaac agtaaaaaac atgtgtgtta
     7261 tttcattttt gattgcagga gcattatttg ctgtattcta tgttcctaat atggttacag
     7321 gtatctatga tactcttaaa aataacccta tagattatac ttttacctac aatcaaagag
     7381 ataaacaggt atcaaaaaaa gatattgaac aattagcaga taaacacaat ataaaaattc
     7441 aaaactatat ggaaattcct tctatagaat taattgttga tggtgtagaa tcaatatatc
     7501 ataaagatgg aactatcgaa aaggtatata tgaaaaaaag ctcctatgat atgttcttta
     7561 attccaaaga tttttccaag ctaattggca aaccaataac tataaaaaat ggtgaatatc
     7621 ttgctgttgt aaataaagga aatgaaaatc aagattataa atataccaaa tttatatcaa
     7681 gcccagtcac agaaaaagaa atccccttta gatatgctgg aaaaataata tttaatggtt
     7741 ctttttttca aaatagaagt atgaacgcct atgtccttaa tgaggaggat tataaatcat
     7801 ttactaaaga tttatccata aaaaattaca ataattctat actatttaat gtagataaag
     7861 atacctatgc ttttgccaat gacttaaaga aagaaattat aaaaagaaca tctaaggaaa
     7921 tgggcatgtt cagtggttat gatgaatacg taaaaaaacg ttatgaatca actggaagaa
     7981 aatattttat ggatgaagaa tatccaagtg gtgaaggtca tttaaaacta gacccaaaca
     8041 acaatcagtt gtatcttaat tggaaatact atcctaactt taaaatttta caaagccaag
     8101 atatgataaa aaacatggca gtcttcttaa tgttattcat atatgtggca atcatatgtt
     8161 ttacagcagt aggaatcatt gcatatacaa ggggtgttac tatagctata aatcacaagc
     8221 aggtctttat ggatttaaat cgattaggag caaacaatga ttatataaag ttctgtatta
     8281 gaaatcaatt aaagaaagta ttttcatacc ctcttattgc aggaagtttt ttaatttatc
     8341 tgtttaattt tatgattatg ttaaataatg atggtagaat cgtcaatagt gaggtcaatg
     8401 ctcttttgat aaacttaggt tttataggga tttgtggggt ttatatgtac cttatatatc
     8461 tattaactta taagaaattc aaaaaaatta ttggtatttt ataataaaaa aggttattag
     8521 atttttaaag taataaaatc attggtgttt tataataaga aaggttatta gatttttaaa
     8581 gtaataaaat cattactttt tataataata aaagcatttg tattttataa taagaaagat
     8641 tgttagattt tttaataaga aaatcattag cacttcagaa taagtttaat agaatagttt
     8701 taagtgtaag tatactaata taaaaaattt tattttgcta tctggatata attgttattt
     8761 cattttatat atctaggtag caaaatatat tatatactta actatataaa tataaaactt
     8821 gaaaggagat aaaaaattga cttcgatttg tatttgtgat gatgaaaaac aacaaagaaa
     8881 catgctgaat aaaatcatta caagagaaat ggatttgtgg ggctacccct accaaataaa
     8941 agaatattca aatggagaaa gtctagtata caatctagaa cacgaaaatt attacataga
     9001 tattattttt ttagatattg aacttggaaa aagtaatggc gttgacatag caaaaactat
     9061 acgaaaacat tcaaaagata ctataatcat ctttgtcaca ggtttttctg actatgtatt
     9121 tcatggatat gatgtaggag ctttgaacta catcttaaaa ccttataaaa ctgaaaaaat
     9181 atgtaaagta ttaaaagaag cactaaaaaa attagaacaa tggaaagaaa actttattac
     9241 agttcagatt ggaactaatc tttgtaaaat taatacaaaa gatatcctct actttaaaag
     9301 tgagctaaga aaattaactg tagttacctt tgataaaaca ttagaatact acggaaaatt
     9361 aaatgatatg gaaactgttc ttccctctaa ttttatacgt acgcatcaaa ggtatattgt
     9421 caatctaaac catgtagata gtataactca aacttatgcc aaagttcaga atgaaaaaat
     9481 tcctataagc cgtaaaaaat atcaagaggt tgtaggaaaa tttacaaggt atatgttaga
     9541 atcttaattt ttgaagttca atctctagaa ttttattttt gaaacttaac tttttaaagc
     9601 ttagttttta gagttttgtt ttttcaaatt taattttttg aagcttaatt tctagaattt
     9661 caatctttca aaatttaatt ttttgaagct tagtttttag agtttttttc aaacttaat
//
LOCUS       DAYAPA010000144         9667 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.148, whole genome shotgun sequence.
ACCESSION   DAYAPA010000144 DAYAPA010000000
VERSION     DAYAPA010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9667
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.148"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            962..2320
                     /locus_tag="ACGI59_003322"
     CDS             962..2320
                     /locus_tag="ACGI59_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HFG9741092.1"
                     /translation="MKAKSTSKFELDGIPPLKEAIPLGLQHLMAMVIGSCLPAILIAN
                     AAGLNHKMSTLLIQAALVFAGLSTLLQLYPIPLFKGFKLGGGIPVIMGATAMFIGAGV
                     SVASKYGLPVLFGSQIIAGIVIIIIGFGLKKIRFIFTPAVSGTIVACMGLGLFPIAIK
                     NLAGGMGNETFGNPINFIVGISVSLMILGLNKYGKGLFKDASILVSIIFGYILSLILG
                     IVNFSSIQEFTLVALPKPLAFGLDIRLEVVVMFSIIYLVEIADIMGACTLSAVGGLNR
                     QVTDEELSSAVLGNGVLSTIGALFSATPMGMFGQNSAIVSNTKVVSKFVLAIGGIGLF
                     LAGISPLLANLIRTIPPCVVGGATLVIFSTLTTSGLRLVSMDGFNQENSMILGLSMAS
                     GIGFMVAPQVLEKFPKFIETLLADSSVVSGAMVAIIIQALYMVKFPGNKSDKVEDKNK
                     SL"
     gene            2427..3221
                     /locus_tag="ACGI59_003323"
     CDS             2427..3221
                     /locus_tag="ACGI59_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HFG9741093.1"
                     /translation="MVTIKEYSVPQSLEEAYKILISKKNNVILGGCGFIKLSNKNIGT
                     AIDLKDINLNYIKEDKKNILIGADTSLRSLEIDKTIKNYCSGILSSAVSNIVGVQFRQ
                     GARIGASVFSKYGFSDLIPALLVVGAKVRLYNKGILELSEFLDSELSRDILVEVILPK
                     KDAIAVFDSIRKCTGDFSVLNSAMLKENDTYKIAIGARPQKARLALEASNILNKEKDI
                     DKASIVASKELTYGSNMRASKEYRKDMASALVKRMYNAIEEGMYND"
     gene            3214..5520
                     /locus_tag="ACGI59_003324"
     CDS             3214..5520
                     /locus_tag="ACGI59_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HFG9741094.1"
                     /translation="MINKGIRKIDSTAIVTGKPLYTEDLIMHKDVLTLKLLRSPHAHA
                     KIKNIDTSKAEKIPGVVGIYTYKDVPQVRYCVPGQTEPEASPYDRVLLEDTVRFIGDE
                     VALVAAEDEKTALKALKMIKVDYEVLKPVFDAREAEGNEVVIHPEKDIVEPYPFGLDA
                     KNNIVSKEFFEFGDVEAKLKESDFVVESSYKTQSQSHCMMETHRSYSYKDINDRIVVV
                     SSLQSPHNVKRILRKVLGIPSSKIRVIKPRIGGGFGGKNIAATDIFTAFVTMKTGRPA
                     KFVFTRSETFVGSNSRHEMFFDVKVGADKEGNLKAIELKSLNNTGAYGGNGVSVSSES
                     GHNTLPIYNDIDAVRFNARTVYTNRLPAGALRGYGATQGIFAIDSAIDELAHKIGMDP
                     LEFRIKNIIDRYTNGKAAEDEILSCELRQCIEKGKKLINWDDKYPCKDIGNNKVRAVG
                     MAVANHGSGIPRVDMATVTIRMDEDGTYKILSGLADLGQGADTIQAQIAAEALNTTVD
                     RISIYTGDSDLCPYDTGAFASCTTTVTGNATIKTANKLKDILLSVAESKLGFKKEDLE
                     LGKDRVFVKNDNSKFVLLEELGRESVGGIGFASDDVPVVLEATESFGMLKKIKPFIAG
                     FAEIELDKNTGEYKVINFACVPDCGTVINPTLARIQVEGGVMMGIGFAMYEDPIFDKD
                     GRLLTDSFLQYKVPTKKEVGNILVDFAENFDPGGPYGAKSLGEIVIHTPAPAIANAIF
                     NATGVRLRELPMTFEKVYMGMKNNNKQL"
     gene            5659..6936
                     /locus_tag="ACGI59_003325"
     CDS             5659..6936
                     /locus_tag="ACGI59_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFG9741095.1"
                     /translation="MSNRIKNIHYGTQRQQLKNLTKKEYQALREMCFLAKNLYNVGTY
                     NVRQHYFNQGEHLNYEANYHVCKENENYKLLNSNIAQQILKEVDSSFRSFFGLIKLAK
                     EGKYDFRGIKSPKYLDKEGFFSIIIGQIRIKEDGILNVPMSPAFKKLYGKVSIKVPQN
                     ILEKKIKEIRIIPKHKARFFEIQYCYEIPKSEEVSNQKNALAIDLGLENLCTCVTNLG
                     DSFIIDGKRLKSVNQWANKQNDKIEALKLKKNITAITRKQFKVWNKRNNQVKDYVNKT
                     CFYIIDYCVKNNIGNLIVGYNAKLEKSDNMERKIYRNFIRIPFGEIKGKLEYLCKIKN
                     INFIRQEESYTSKSDFFADDFLPDINVDNVKKYDFKGERITRGQYKSSVGIIINADVN
                     ASFNILKKSQVVNLSNLQNNPQILKQPQRVRIS"
     gene            7119..7841
                     /gene="yqeC"
                     /locus_tag="ACGI59_003326"
     CDS             7119..7841
                     /gene="yqeC"
                     /locus_tag="ACGI59_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HFG9741096.1"
                     /translation="MELSNIIEKNEIITVVGAGGKTSFINYFANFYRNKLKVLLTTTT
                     KIYVPNDYDNIIITIDGTVIPSICHGITVCGSYINNENKLVSIDSSILDEIVDQFDLV
                     LIEGDGSKRKKLKGWNAKEPVVYHRTTKTIGILDITSFGMNINEENIHRVEIFKKIAN
                     LDTSSINSSSTVSIENLKNIVLNPNGLFKNYSGKRVLFINKVENEKYKNLAIKLIENI
                     KEYESEIEIFYGSVKQKFCVRY"
     gene            7948..8517
                     /gene="mocA"
                     /locus_tag="ACGI59_003327"
     CDS             7948..8517
                     /gene="mocA"
                     /locus_tag="ACGI59_003327"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HFG9741097.1"
                     /translation="MINAVIMASGFARRMGANKLLLNYKGCSIIEHVFREVSKIDFHQ
                     VVVVSQYEEVLLLCNKYGFEYIDNKNANIGQSESIKLGVLNSSKCDGYMFFVGDQPFI
                     DNLYIDKIINTFKLDRDFIVIPRYKDMCGNPVIFPYSKKEELLALKEDEKGKTILKNS
                     SKIKYVEVPKEMLFDIDTKMDYERIGGKL"
     gene            8514..9533
                     /locus_tag="ACGI59_003328"
     CDS             8514..9533
                     /locus_tag="ACGI59_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HFG9741098.1"
                     /translation="MKLIKTIDAVGQVLCHDITQIIPGEFKGRKFKKGHIVKEEDIPV
                     LLSLGKDNLYVWEKSEGMVHENEGALFLKELTAGENLDFSEIKEGKIDFIAACDGLLK
                     IDVDALFDLNCVGEIMMATLHNNFIVNKGLKVAGTRVIPLVIDEKKLEDAKKVVGNRK
                     IVNVVPFKPKKVGIVTTGNEVFYSRIVDKFGPVIEEKVKGFGCEVIGQTICPDDKEII
                     KSAIKEFISQGAELICCTGGMSVDPDDVTPTAIKETGADLVTYGSPILPGAMFLLAYY
                     GEVPIMGIPGCAMYHKTTVFDIVLSRVLIDEKLDKYDIARYGHGGLCMNCDVCTYPAC
                     NFAKI"
BASE COUNT         3825 a         1006 c         1808 g         3028 t
ORIGIN      
        1 agatagaaac ttgtatattt tatgttgcag atacaaatat agtaaaggag agtacattgg
       61 tacaactgag atacatagct tattaagttt ttctgtctta tgagaattat attataaaat
      121 attcataaat attttgagtt taaattaaat aattatttta tgcttagatt aattgatatt
      181 taattaattt aataacaaaa acagtgaaag agtaaataat attatttaca gggggtaaag
      241 caaagtatgt taaataaatt gtacttttgg ccccgtacat agagaaatct atgtaggaca
      301 caactttaaa accagtaaat cctaaagcct tgcaactaca ataaaagata gaaagaaact
      361 tttatgaatg tggtgaaaac cagaaacaat agtgaggatg gtatatggtg agagcctaag
      421 tacttaaaaa tggacatttg gtcgccaagc ctagaaatag gaaggtcaaa cgactatctc
      481 gagagagagt acactataag cgattgatag tggaaacggg ttgctcttag caggtaatgc
      541 tgaagatgaa gaaatagtct acgctcatat gaaagtatga ggtcactgga tttgaagtga
      601 catctataaa gtagcgttta taggtaaatg tttaaaactt gaaacatgta aatacttatg
      661 aaaacttata acttataatg attaagattg aaagatttaa attgaattaa atttactata
      721 aaaaattaaa aattcatagc tataatactc ttatatttgt aaagcatggt atataatgta
      781 tacaaaatta aagaatacgt gtatttgtta ataattaaat ttattgcatc tgttttttaa
      841 gactaaaaac ttaaatatat gttggagtat agttgatatt tatgaacatc attaatttaa
      901 tatattagat ttttaagtat ttatataatt caataagttt tatgtaacgg gagtttttat
      961 tatgaaagca aaaagtacat caaaatttga attagacgga ataccaccac taaaggaagc
     1021 aataccttta gggttacaac acttaatggc aatggtaata ggtagttgtt taccagcgat
     1081 attgattgca aatgcagctg gacttaatca taagatgtca acattattga ttcaagccgc
     1141 attagttttt gcaggattat caactttatt acaattatat ccaatacctt tgtttaaagg
     1201 ttttaaatta ggaggaggaa taccagttat aatgggagct acagctatgt ttataggagc
     1261 tggggtatct gttgcaagta agtatggact accagtatta tttggctctc aaataatagc
     1321 tggtatagta ataattataa ttggttttgg tcttaaaaaa atacgattta tatttactcc
     1381 agctgtatca ggaacaatag ttgcatgtat gggtcttgga ctttttccaa tagctataaa
     1441 aaacttagct gggggtatgg gcaatgagac ttttgggaat ccaattaatt ttatagttgg
     1501 aatatctgta tctcttatga tattaggatt aaataagtat ggaaaaggat tatttaaaga
     1561 tgcatcaata ttagttagca taatttttgg atacatttta tcattaattt tgggtatagt
     1621 aaatttttct tcaattcagg agtttacttt ggttgcactt ccaaaaccac ttgcttttgg
     1681 gctagatata agacttgaag ttgttgtaat gttttcaata atatatttag ttgagatagc
     1741 tgatataatg ggtgcttgta cactttctgc ggtaggaggg cttaatagac aagtaacaga
     1801 tgaagaactt tcttctgctg ttttaggaaa tggagtactc tctacaatag gtgcattatt
     1861 ttctgcaact ccaatgggga tgtttggaca aaattctgca atcgtttcca atacaaaggt
     1921 tgtaagtaaa tttgtactag ctattggagg aattggatta tttttggcag gaatatcgcc
     1981 tttattagct aatttaataa gaacaattcc tccctgtgtg gttggaggag caacactcgt
     2041 tatattttca actcttacaa catctggttt aagacttgtt agtatggatg gatttaatca
     2101 agaaaatagc atgatattag gtctttctat ggcttctggt attggattca tggtagctcc
     2161 tcaagttctt gaaaaatttc caaaatttat agagacatta cttgcagatt caagtgtagt
     2221 ttcaggagca atggtagcca taataattca agctttatat atggtaaaat ttcctggaaa
     2281 taaaagtgat aaggttgagg ataaaaataa aagtttataa tgtaaaggtt taatatatgt
     2341 gaattaaata tactacaatt ataaatataa ataaaagatt tattaaaaac ttttgtgttt
     2401 taataagtta atgatggggg gataagatgg ttactataaa ggaatattca gttccacaga
     2461 gtttagaaga agcatataaa attttaattt ctaaaaaaaa taatgttatt ttaggtggat
     2521 gtggatttat aaaattaagt aataaaaata taggaacagc gatagattta aaagatataa
     2581 atctaaatta tataaaagag gataaaaaaa atattttgat aggtgcagat acttcactta
     2641 gaagtttaga aattgataaa actataaaaa attactgtag tggaatactt tcaagcgctg
     2701 tttctaatat agttggtgtt caatttagac aaggggctag aattggtgca agtgtatttt
     2761 caaagtatgg cttttctgac ttaataccag cacttttggt tgttggagcg aaggtgagat
     2821 tatacaataa aggaatacta gagttaagtg agtttttgga tagtgaatta agtagggata
     2881 tattggtcga agtaatacta ccaaaaaaag atgcaatagc tgtttttgac tcaataagaa
     2941 aatgcacagg agatttttct gtattaaatt cagcaatgct aaaagaaaat gatacttaca
     3001 aaatagcaat tggggcaaga cctcaaaaag ctagattagc acttgaagcg agtaatatat
     3061 taaataaaga aaaagatata gataaggcat ctattgttgc tagtaaggaa cttacttatg
     3121 gaagtaatat gagagctagt aaagagtaca gaaaggatat ggcaagtgca ttagttaaaa
     3181 ggatgtataa tgctattgag gagggaatgt ataatgatta ataaggggat aagaaagata
     3241 gatagtacag caattgtcac aggaaaacct ctatatactg aagacctcat tatgcataaa
     3301 gatgttttga cattaaaact acttagaagt ccacatgctc atgctaagat aaagaatata
     3361 gatacatcaa aagccgaaaa aattccaggg gttgtgggta tttatacgta taaagatgta
     3421 ccacaggtca gatattgtgt tccaggtcaa acagaaccag aagcatcacc ttatgataga
     3481 gttttacttg aagatacagt tagatttatt ggagatgaag tagctttagt ggctgctgaa
     3541 gatgagaaaa cagcacttaa agccttaaaa atgataaagg tagattatga ggttttaaag
     3601 cctgtgtttg atgcaagaga agcagaagga aatgaagttg ttatacatcc agaaaaagac
     3661 atagtagagc catacccttt tggattggat gcaaaaaata atatagtatc aaaagaattt
     3721 tttgaatttg gagatgttga agctaagctt aaagaaagtg attttgtggt tgaatcttca
     3781 tacaaaaccc aaagtcaatc gcattgcatg atggaaactc ataggtcata ttcatataaa
     3841 gatataaatg atagaattgt tgtagtttca tcattacaat caccacacaa tgtaaagaga
     3901 atacttagaa aagtattggg tataccgtct tcaaaaataa gagtaattaa acctagaata
     3961 ggtggtggat ttggaggaaa gaatatagca gcaacagata tatttactgc atttgttact
     4021 atgaaaactg gtagaccagc aaaatttgta tttacaagaa gtgaaacttt tgtaggttct
     4081 aatagtagac atgaaatgtt ttttgatgtt aaagttggtg cagataaaga aggaaattta
     4141 aaagcaatag agctaaagtc attaaacaat acaggtgctt atggtgggaa tggtgtctct
     4201 gtttcttccg aatctggaca caatacctta ccaatatata atgatattga tgctgttaga
     4261 tttaatgcta gaacagtata tactaatagg ttaccagcag gagcacttag agggtatggt
     4321 gctacacagg gaatatttgc aatagatagt gctattgatg aattggcaca taagattgga
     4381 atggacccac ttgaatttag aattaaaaat ataatagata ggtataccaa tggaaaagca
     4441 gctgaagacg aaatattaag ttgtgagctg agacaatgta tagaaaaggg aaagaaatta
     4501 attaattggg atgataaata tccatgtaaa gatataggaa acaacaaagt aagagcagta
     4561 ggtatggcag tagcaaatca tggttctggt ataccgcgtg tagatatggc aacagtcact
     4621 ataagaatgg atgaagatgg cacatataaa atattatctg gtcttgctga cttaggtcaa
     4681 ggggctgata caattcaagc tcaaattgct gcggaagctt taaatactac tgtagataga
     4741 atttctattt atacaggtga ttcagactta tgtccctatg atacaggagc atttgcttct
     4801 tgtacgacaa cggttacagg aaatgcaaca ataaagacag caaataaatt aaaagatata
     4861 ttactttcag ttgcagagtc aaagctagga tttaaaaaag aagatttaga gttgggaaaa
     4921 gacagggttt ttgttaaaaa cgataatagc aagtttgttc tattagagga attgggtaga
     4981 gaatctgttg gaggaatagg atttgctagt gatgatgtac cagtagtttt ggaagcaaca
     5041 gaatcttttg gcatgttgaa aaaaataaaa ccatttatag caggatttgc agagatagaa
     5101 ttagataaaa atacaggtga atataaagtc ataaattttg catgtgtacc tgattgtgga
     5161 acagttataa atccaacttt agctagaata caagtagaag gtggtgtgat gatgggtata
     5221 gggtttgcaa tgtatgaaga ccctatattt gataaagatg gtagattatt aacagatagt
     5281 ttcctacagt ataaagttcc tacaaaaaaa gaagttggaa atatattggt agattttgct
     5341 gagaatttcg acccaggagg tccttatggt gctaaatcat taggagaaat agttatacat
     5401 acaccagcac cagctatagc aaatgcaata tttaatgcta caggagttcg tttaagagag
     5461 cttcctatga cttttgaaaa agtttatatg ggtatgaaaa ataataataa acaattataa
     5521 taaataataa gatgcataaa agcaagtaaa tttaaattat tttgtaaaat aaagtaatta
     5581 aatggacatt atatatggtg ttttgtaaaa ataccgtata taatgtctag tagagtatat
     5641 tctaaaggag gtgagttcat gtcaaacaga ataaaaaata tacactatgg aactcagaga
     5701 caacaattaa aaaacttaac taaaaaagaa tatcaagctc tacgagagat gtgcttctta
     5761 gctaagaact tatataatgt tggtacatat aatgtaagac agcattattt caatcaagga
     5821 gaacatctta actatgaagc taattatcat gtatgcaaag aaaatgagaa ctataagctt
     5881 ctcaatagta atatagcgca acaaatatta aaagaagtag acagttcgtt taggtcattt
     5941 tttggattaa ttaaattagc caaagaagga aaatatgact ttagagggat aaaatcacct
     6001 aaatatttag ataaagaagg attttttagc ataataatag gtcaaataag aatcaaagaa
     6061 gatgggattt taaatgtacc tatgtctcca gcatttaaaa aactatatgg gaaagtaagt
     6121 ataaaagttc cacaaaatat actagagaaa aaaattaaag aaataagaat aatacctaaa
     6181 cataaagcta ggttctttga aattcagtat tgttatgaaa taccaaaatc agaggaagtt
     6241 tcaaatcaaa aaaatgcact agctatagac ttgggtttag aaaatttatg tacatgtgtt
     6301 accaatttag gtgatagttt tatcatagat ggaaaaaggc taaaatctgt gaatcagtgg
     6361 gcaaacaaac agaatgataa aatagaagcc ttaaaattaa aaaagaatat aactgcaata
     6421 acaagaaaac aatttaaagt ttggaataag agaaataatc aagttaaaga ctatgtaaat
     6481 aaaacctgtt tttatataat tgattattgt gtaaaaaata atattggaaa tttaattgta
     6541 ggatataatg ctaagttaga aaaaagtgat aatatggaga gaaaaatata tagaaatttt
     6601 ataagaatcc catttggaga gataaaagga aaattagaat atctgtgtaa aataaaaaat
     6661 atcaatttta ttaggcaaga agaaagctat actagtaaat cagatttttt tgctgatgat
     6721 tttctacctg atatcaatgt ggacaatgtt aaaaaatatg actttaaagg ggaaagaata
     6781 acacgtggac agtacaaatc cagtgttgga atcattataa atgcagatgt aaatgcgagt
     6841 tttaatatac taaaaaaatc acaagtagtt aatttatcta atttacagaa taatcctcaa
     6901 atattaaagc aaccacagag agtaagaata tcttaattat atttaaaaga taaactgata
     6961 gcgtaagctt acagcttagc ggcgaattgg atccgccttg ttgtttacaa cagaatctat
     7021 ttactttcga gtttatggaa tttcaaaaat cacttttact aaaaaggcct aactataata
     7081 attaagagat tagaaaactg gttataagag gtgaaagtgt ggaactatca aatataatag
     7141 aaaagaatga aataataact gtagttggag caggaggaaa aacttcgttt ataaactatt
     7201 ttgcaaattt ttatagaaat aaattgaaag ttcttcttac aacaacaact aaaatatatg
     7261 taccaaacga ttatgacaat ataattatta caatagatgg aactgttatt ccatctattt
     7321 gtcatggaat aacagtctgt ggcagttata taaataatga aaataaatta gtaagtattg
     7381 attcctctat tttggatgaa attgtagacc aatttgattt agttttaata gagggagatg
     7441 gctcaaagag aaaaaaactt aaaggttgga atgctaaaga accagtagta tatcatagaa
     7501 ctacaaaaac catagggata ttagatataa catcatttgg aatgaatatt aatgaagaga
     7561 atatacatag agtagaaata tttaaaaaaa tagcaaattt agatacaagt agtattaata
     7621 gtagttctac agtaagtatt gaaaacttaa aaaatatagt attaaatcca aatggattat
     7681 ttaaaaatta ttctggaaaa agagtattat ttattaataa agttgaaaat gaaaaatata
     7741 aaaatttagc tataaaatta atagaaaata ttaaagaata tgaaagtgaa atagagatat
     7801 tttatggaag tgtaaaacaa aaattttgtg taagatattg aagtattaaa atagtttata
     7861 tgatattata tatatagata atatcaatag agatattata aaaatcatag aactatcaat
     7921 atattataaa taccaaggat gatatatatg ataaatgcag tgataatggc atcaggcttt
     7981 gccagaagaa tgggagcaaa taaactttta ttaaattata aaggctgttc tataatagaa
     8041 catgtattta gagaagttag caagattgat tttcatcaag ttgtagtagt aagtcaatat
     8101 gaagaggttt tgttattgtg taataaatat ggttttgaat atatagataa taaaaatgca
     8161 aatataggtc aaagtgaatc tataaaatta ggagttttaa atagctcaaa atgtgatggt
     8221 tatatgttct ttgtaggaga ccagccattt attgataact tatatataga taaaattata
     8281 aatactttta aattagatag agattttata gtaattccaa gatacaaaga tatgtgtgga
     8341 aatccagtca tattcccata tagcaagaaa gaagaattgt tggcacttaa agaagatgaa
     8401 aaaggtaaaa ccattttaaa aaattcatca aaaataaagt atgtagaagt tcctaaagaa
     8461 atgttatttg atatcgatac aaaaatggac tatgagagaa ttgggggtaa attatgaagc
     8521 taataaaaac tatagatgct gttggacaag ttttatgtca cgacataact caaataatac
     8581 ctggagaatt taaaggaaga aaatttaaaa agggtcacat agtaaaagaa gaagatattc
     8641 cagtgttatt atctcttggt aaagataatt tgtatgtatg ggaaaagtca gaaggaatgg
     8701 ttcatgaaaa tgaaggcgca ttgtttttaa aagaactaac tgctggagaa aatttagatt
     8761 ttagtgaaat aaaagaaggg aaaatagatt ttatagcagc atgtgatggt ctattaaaga
     8821 tagatgttga tgcacttttt gatttgaatt gtgttggaga aataatgatg gctactcttc
     8881 ataataattt tatagtaaat aaaggtttaa aagtagctgg aacaagagtg atacctttag
     8941 ttatagatga gaaaaaatta gaagatgcaa aaaaagtagt tggtaataga aaaatagtaa
     9001 atgtagtacc ttttaaacct aagaaagtag gaattgttac tacaggaaat gaagtgtttt
     9061 atagcagaat tgttgataaa tttggaccag taatagaaga aaaagtaaaa ggctttggtt
     9121 gtgaagttat aggacagact atatgcccag atgataaaga aattataaag agtgcaataa
     9181 aagaatttat aagtcaagga gcagaactta tctgttgtac tggtgggatg agtgtagacc
     9241 cagatgatgt tacgccaaca gctataaaag aaactggagc tgatttagtg acatatggtt
     9301 caccaatatt accaggagct atgtttttac ttgcttatta cggagaagtt ccaattatgg
     9361 gtataccagg atgcgcaatg tatcacaaaa ctacagtatt tgatatagtt ttatctaggg
     9421 ttttaattga tgaaaagctt gataagtatg atatagctag atatggtcat ggtggccttt
     9481 gtatgaactg tgatgtatgt acttatcctg catgtaattt tgctaaaata taaagttatt
     9541 aaaaaagagt atttacaaat taagtactct ttttaaatat gaaacaaaac agctagaatg
     9601 atgttttaaa aaaagcatat ttatttagaa attataataa cttattttca atgtgtaaaa
     9661 gaatgaa
//
LOCUS       DAYAPA010000145         9240 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.149, whole genome shotgun sequence.
ACCESSION   DAYAPA010000145 DAYAPA010000000
VERSION     DAYAPA010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9240
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.149"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            442..1647
                     /locus_tag="ACGI59_003329"
     CDS             442..1647
                     /locus_tag="ACGI59_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFG9741099.1"
                     /translation="MSKKVLIMSASTGGGHNRAALAIKEELTSKTLDGEPIECEIIDS
                     LKLVNNTMDKIISRGYEKSAIYTPKAYGSVYRLSETNLLSKNEFKDNLLITFMAKKFK
                     KLIRSEKPDLIIGTHPFPMIALSTLKKNFNLHNNESNAYTEHFYKHYTNTINVPPLIS
                     VLTDYTTHSTWIQNEIDYYIVGHEYVKELLVFDGVEPSKIRTFGIPVEKSFLSHRDKG
                     IVLSELNLSPDKLTVLLMGGSFGAGNIKETLDELLDTDRDFQILVITGKNESLKEKIE
                     KKLMSRYHDKNVCVLGYTDKMNDILASIDVLVSKPGGLTTTEALLKDVPMIVPYYIPG
                     QEEENLDFLSNCGAALRTTKKYNLSILLKVLIDDPSRLEMLKRNIKSIRKSNSAQNIA
                     NLILNIISG"
     gene            complement(1718..1939)
                     /locus_tag="ACGI59_003330"
     CDS             complement(1718..1939)
                     /locus_tag="ACGI59_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9741100.1"
                     /translation="MKPIMNLEEHNAMKEKRVKKMKSKDKPKYDDFEDISFSNKKKTK
                     KNVRNKNAEFRNNYKNYQYDYEEDFYEPI"
     gene            complement(2080..2679)
                     /gene="recR"
                     /locus_tag="ACGI59_003331"
     CDS             complement(2080..2679)
                     /gene="recR"
                     /locus_tag="ACGI59_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFG9741101.1"
                     /translation="MQVYTGPITRLIEEFSKLPGVGRKTAQRLAFHIINMNTNDVEAL
                     SKAIIDAKREIRYCSICCNITDTDPCSMCSNKSRDSSVICVVEDPRDVAAMERTREFK
                     GQYHVLNGVISPMDGIGPDMLKIKELIQRLGNQEVKEIIMATNPTIEGEATAMYIARL
                     VKPMGIKVTRIAHGLPVGGDLEYADEVTISKALEGRREI"
     gene            complement(2692..3042)
                     /locus_tag="ACGI59_003332"
     CDS             complement(2692..3042)
                     /locus_tag="ACGI59_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFG9741102.1"
                     /translation="MAKKGFGGGMMPGGGNMNNLLKQAQKMQENMQKAQQELESKEVE
                     ASVGGGAVTVKVNGKKEVIDITIKPEVVDPDDIEMLQDLVLSAVNQALRNIDDIQASQ
                     MSKVTGGMNIPGLF"
     gene            complement(3101..4738)
                     /gene="dnaX"
                     /locus_tag="ACGI59_003333"
     CDS             complement(3101..4738)
                     /gene="dnaX"
                     /locus_tag="ACGI59_003333"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFG9741103.1"
                     /translation="MHKALYRAYRPQKFEDVIGQDHIIKTLKNQIYSDNIGHAYLFCG
                     TRGTGKTSTAKIFSRAVNCLNKINEEPCNECEICESVLKDNTMDVVEIDAASNNSVDD
                     IRELRESVKYSPANAKYKVYIIDEVHMLSQGAFNALLKTLEEPPSYVIFILATTEPHK
                     IPATILSRCQRYDFKRVTVKDMTLRMKKICEDEGIDIDDKALNLIARNSQGALRDALS
                     ILDQCMSFGESKIDYKDVVELMGSVNIEQLFELSQCIVEQDTKKSLEILNEFVLWGKD
                     IRNLINDLIDHFRNLMVCKVSSELDEIISLPEETIEQLKIQSKNIDINDLIRILNILS
                     ITQDDIKSSSNPRVLVEITIMKIAQPMFDESKEALIKRVENLEKMIELGNFKSEKIGN
                     NKEKEYEVDREIDVKQENVVYEDVKNEDVILIESSWKNILKQIKKDKKMPIYALLSEV
                     KSFNVYSNMLYVIFDDKFDFAKTRLSSQDTINYLEKTIRDVLNRSFNVKIVLTSEVKD
                     INLEVKEKKDIGEEILKNIVSEEILEIKDSIDENESK"
     gene            complement(4815..5079)
                     /gene="ffs"
                     /locus_tag="ACGI59_003334"
     ncRNA           complement(4815..5079)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACGI59_003334"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            complement(5117..5208)
                     /locus_tag="ACGI59_003335"
     tRNA            complement(5117..5208)
                     /locus_tag="ACGI59_003335"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5172..5174),aa:Ser,seq:gga)
     gene            complement(5209..5664)
                     /locus_tag="ACGI59_003336"
     CDS             complement(5209..5664)
                     /locus_tag="ACGI59_003336"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HFG9741104.1"
                     /translation="MESSFYMKEALKEAYKAYNKKETPIGAIIVKDNQIIARAHNLTE
                     TLKDSTAHAEILAIKQASEKLGGWRLTDCDLYVTMEPCIMCSGAIVNSRIKKLIIGTR
                     HVKNSYIEKQHEFKLDYFNNNNVKVAFDVLQEECSIILQEFFKALRKRD"
     gene            complement(5985..7256)
                     /gene="serS"
                     /locus_tag="ACGI59_003337"
     CDS             complement(5985..7256)
                     /gene="serS"
                     /locus_tag="ACGI59_003337"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006441208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFG9741105.1"
                     /translation="MLDIKRIRENLDDIKKAMERRGEREFDLDAVVELDNKRREILQE
                     VEVMKNELNVNSKKIPQLIKEGKDVTEEKARLKELSDKIKGIDEKVKEVEAKMEYTLM
                     RIPNVPHPEVPQGETDEDNVQIRTWGEPTKFDFEHKAHWDIGTGLGILDFERAGKITG
                     SRFTLYRGLGARLERSLMNFFLNTHTAKHGYTEVLPPFMANRNSFIGTGQLPKFEEDM
                     FKIEGLEYFLIPTAEVPVTNIHANEILDVAELPIKYCAYTPCFRSEAGSAGRDTRGLV
                     RQHQFNKVELVKFVKPEDSYNELESLTHDAETMLQMLGLPYRVVKICTGDLGFTAAFK
                     YDLEVWMPSYNRYVEISSCSNFEDFQARRAGIRFKRDKKSKAEYVHTLNGSGLAIGRC
                     LAAILENYQQADGSVVVPEELRPYMGVDVIK"
     misc_binding    complement(7382..7587)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(7715..8596)
                     /locus_tag="ACGI59_003338"
     CDS             complement(7715..8596)
                     /locus_tag="ACGI59_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HFG9741106.1"
                     /translation="MNFLSLAKTASTNSNITKRTLENMLDNFSDNIPNIIYAIIVFVI
                     GIYISKIVRRMVSKFLIKYGMSKGVNNFIVYGIYISMLSIISLISLGIIGIQTTSVVA
                     VLGAAGFSIGLAFKEILSNLGSGMIILFFKPFNIGDYIQGSGVEGTVSDIQIFSTVLK
                     TPDNKTIIIPNFQLTSNNIINYTHQNKRRIDFSYNISYDSDIDVVKSILNEIFTNEKR
                     ILNDPKPIIGLNSIGNNTMQIVARPWVKTDDYWDVYFDVMEKVKNKFDENDIKVPFVP
                     SSLLFNNTGNLNTMDKR"
     gene            complement(8608..9117)
                     /locus_tag="ACGI59_003339"
     CDS             complement(8608..9117)
                     /locus_tag="ACGI59_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HFG9741107.1"
                     /translation="MNSNERRSKLIDILKESKHPVKGGTLAELLNVSRQVIVQDIALI
                     RARGFEIIATPQGYIIYNQPYFTKQIKCKNHKDSKEIYDELKIIVDLGGIIKDVIVNH
                     PTYGQITAELNIYSKMDIDNFMKKVETNEFKQLSVLTECSHIHTIEAIKEETIEAIVK
                     ELKDNGILS"
BASE COUNT         2956 a         1604 c          977 g         3703 t
ORIGIN      
        1 atataaatta acctactgtt taattttcaa tgttcgtgtt tatttctttt tccttttttc
       61 aaggacaagt aatactatat catctttatc tattctagtc aatataattt tttacttttt
      121 ttattttttt tatttatcat gtattcttta tatttaattt ttaaaactta atgctaagta
      181 aacctgttta tttcataaaa ctaatatata ttctatgctc tattttcctt tttattctat
      241 atttaaattt tttatttaca agaatatttt taatataaca tatttgataa aattaattta
      301 catttctttc acttgtaatt tttgcaatct tgtattagat taatattaaa aataatttat
      361 caataacctt attcacaaag acagttttta tatatcaaaa tataatataa atactatgct
      421 acattttagg aggattttat tatgagcaaa aaagtattaa taatgtctgc ttccactggt
      481 ggtgggcata atagggctgc tctagctatt aaagaagaac ttacttccaa aacacttgat
      541 ggagagccaa ttgaatgtga aataatagat agtttgaaat tagttaataa tacaatggat
      601 aaaataattt caagaggata cgaaaaatct gctatatata ctcccaaagc ctatggaagt
      661 gtatatagat tatctgaaac aaatctttta tcaaaaaatg aatttaaaga taatctacta
      721 ataacattca tggctaaaaa atttaaaaaa cttattagaa gcgaaaaacc agacttgatt
      781 ataggaacgc atccttttcc aatgattgct ctaagtacat tgaaaaaaaa ctttaattta
      841 cataataatg aatcaaatgc ttacacagag catttttata aacattatac aaataccatt
      901 aatgttcctc cgcttatttc tgtattaacc gattatacta ctcattctac atggatacaa
      961 aatgaaattg attactatat tgttggtcat gaatatgtaa aagaattatt ggtgtttgat
     1021 ggagttgaac cgagtaaaat tagaacattt ggaatccctg ttgaaaaatc ttttctctct
     1081 cacagagata aaggtattgt tctttctgag ttgaatttat ctcctgataa attaactgtt
     1141 ttacttatgg ggggtagctt tggggctgga aatataaaag agacacttga tgaattactg
     1201 gatactgata gagactttca aatacttgta attactggta agaatgaaag cctaaaagaa
     1261 aaaatcgaaa aaaaacttat gtctcgttat catgataaaa atgtatgtgt tcttggatac
     1321 actgataaga tgaatgatat tttagcttct atagatgtat tagtaagcaa acctggtgga
     1381 cttactacaa ctgaggctct tttaaaagat gtacctatga tagttcctta ttatatacca
     1441 ggtcaggaag aagagaatct tgatttctta tctaattgtg gtgcagcact tagaactaca
     1501 aaaaaatata acttgtcaat attactaaaa gttttaatag atgaccctag cagattagaa
     1561 atgctaaaaa gaaatattaa atcaataaga aagtcaaatt cagcccaaaa tatagctaat
     1621 ttaattttaa atataatttc tggttgataa aataaataaa atccctatag tatatatatt
     1681 atctattagg gattttttat tttctaatta tttaatatta aattggttca taaaagtctt
     1741 cttcataatc atactgataa tttttataat tatttctaaa ttcagcattt ttatttcgta
     1801 catttttttt agtttttttc ttattagaaa aacttatatc ctcaaaatca tcatatttag
     1861 gtttatcttt tgatttcatt tttttaactc ttttttcttt cattgcatta tgttcttcta
     1921 agttcataat tggtttcatg ttgcacccct atcaaaattt ttaaatgatt tatacaacaa
     1981 attatacaat acagaagaaa tttattcaat aaatctaaat caaaattaat ttgaatttaa
     2041 ctcaaaaaat tacaaaaatc gaggtattaa cctcgatttt tatatttctc ttctgccttc
     2101 tagtgctttt gatattgtga cctcatctgc atactctaaa tcgcctccaa caggaagacc
     2161 atgagctatc cgagtgactt tgattcccat tggttttact aacctagcaa tatacatggc
     2221 tgtagcttca ccttcaatag taggatttgt tgccattatt atttctttga cttcctgatt
     2281 gccaagtctt tggataagtt cctttatttt taacatatct ggtccaattc catccattgg
     2341 agaaattact ccatttaata catggtactg tcctttaaac tctctcgttc tctccatagc
     2401 tgctacatct cttggatctt ccacaacaca tataacactt gaatctctac ttttatttga
     2461 acacatgcta catgggtctg tatctgttat attacaacaa attgagcaat atcttatttc
     2521 tctttttgca tctataatcg ctttagataa agcttccaca tcattagtat tcatattaat
     2581 aatatgaaaa gccaatcttt gagcagtctt tcttcctact ccaggaagct ttgaaaattc
     2641 ctctataagt ctcgttatgg gacctgtata aacttgcata aataccacct actagaataa
     2701 cccaggtata ttcatacctc cagttacctt actcatttga cttgcttgta tatcatcaat
     2761 atttctcaat gcttgattta ctgcacttaa aactaaatct tgtagcattt ctatatcatc
     2821 tggatcaact acttctggct tgatagttat atctataact tcttttttac cattaacttt
     2881 tacagtaact gctcctccac caactgaagc ttctacttct ttactttcta actcttgttg
     2941 agctttttgc atattttctt gcattttttg agcctgcttt aacaagttgt tcatatttcc
     3001 accaccaggc atcattccgc ctccaaaacc ttttttagcc ataaacacta ttcctcctaa
     3061 acttattttc attttattat tatatcataa tagtatttta ttatttactc tcattttcat
     3121 caatactatc ttttatttct aatatctctt cactcactat gttctttaat atttcctcac
     3181 caatgtcctt tttctcttta acttcaagat ttatatcttt aacttctgat gtcagtacta
     3241 tttttacatt aaaacttcta tttaaaacat ctcttattgt tttttctaaa taatttattg
     3301 tatcttgaga gctcagtcta gtttttgcaa aatcaaattt atcatcaaat ataacataca
     3361 acatattaga atatacatta aaacttttta cttcacttaa gagtgcgtat attggcattt
     3421 ttttatcttt tttaatttgt ttaagtatat tcttccaaga tgactctata agtataacgt
     3481 cctcattttt tacatcttca taaactacat tttcttgttt tacatctatt tctctatcga
     3541 cctcatactc tttttcttta ttattcccaa ttttttcaga cttaaagttc cccagttcaa
     3601 tcattttttc taagttctca actcttttta taagagcttc tttactttca tcaaacattg
     3661 gttgagcaat tttcattatg gttatttcca ctaaaaccct tggatttgaa gatgacttta
     3721 tatcatcctg agttattgat aatatattta aaatccttat taaatcattt atatctatat
     3781 ttttagactg aattttaagt tgttctatag tttcttctgg taatgaaatt atttcatcca
     3841 attctgatga aaccttacat accattaaat ttctaaaatg atcaattaag tcatttataa
     3901 gatttcttat atctttgccc cataagacaa attcatttaa tatctcaaga gactttttag
     3961 tgtcctgctc tactatacat tgagacaatt caaataattg ttctatattt acgcttccca
     4021 ttaattcaac aacatcttta taatctatct tactttctcc aaaagacata cattggtcta
     4081 atatactaag tgcatctcta agagctcctt gagaatttct agctattaaa ttaagagctt
     4141 tatcatctat atcaattcct tcatcttcac aaatcttttt cattctaagg gtcatatctt
     4201 taactgtaac tcttttaaaa tcgtatcttt ggcatcttga taaaatggtt gcaggtattt
     4261 tgtgaggctc tgtagttgct aatataaaaa ttacatatga tggaggttcc tctaaggtct
     4321 ttaataatgc attaaaagct ccttgagaca acatatgaac ttcatctatt atatatactt
     4381 tatatttagc attggctgga gaatatttca cactttctct aagctctctt atatcatcta
     4441 cactattatt tgaagctgca tctatttcta caacatccat tgtattatct tttaatacag
     4501 attcgcatat ctcacactca ttacacggtt cttcatttat tttattcaaa cagtttactg
     4561 ctcttgaaaa aatctttgca gtagatgttt taccagtacc tcttgttcca caaaacaaat
     4621 aagcatgtcc tatattatca ctatatattt gattctttaa agtcttaata atatgatctt
     4681 gaccaataac atcttcaaat ttttgtggtc tatatgctct atataatgct ttatgcatct
     4741 ttttacctct ctttagcaaa attaataaaa aaatccctca ttatctaagg gattagtctt
     4801 ttatatatta aatgctgtac accatctatc gacttaattc cataggcgtt accaaaacag
     4861 ttagctcaac ttaggcaccc ccacggcaca caaaagtgat cacttaccgc tgcttccttc
     4921 cggatctgac ggggttcatg agctttcatt gcgcaggacc taagcatcaa caccacttat
     4981 ttagggcggc cctacagaaa ataagccttt agatggaatt caatcctgct atagcggatt
     5041 gcaggttaca gggcaccgct acctccccat ctagtacagc aaaattacaa ccatatttaa
     5101 ttactttcat tgtttctggc ggagagagtg ggatttgaac ccacgataca gttttaccca
     5161 tatactcgct ttccaggcga gcgccttcga ccactcgacc atctctcctt aatctctttt
     5221 tctcaatgcc ttaaaaaatt cttgcaatat gatcgagcat tcttcttgca aaacatcaaa
     5281 agctactttt acattattat tattaaaata atctaattta aactcatgct gtttttctat
     5341 atatgaattt tttacatgtc ttgtccctat tataagcttt tttattctag aattaactat
     5401 tgcgccacta cacattatgc aaggttccat tgtaacatat aaatcacaat cagtcaacct
     5461 ccaaccacct aatttttctg atgcttgttt tatcgctaaa atttctgcat gtgccgtaga
     5521 gtcttttaac gtttctgtaa ggttatgagc tctagctatt atttgattat ctttaactat
     5581 aatagcacct attggtgttt cttttttatt atatgcttta tatgcttctt ttaatgcttc
     5641 tttcatataa aatgaacttt ccataattcc tcccaaaact tataacaaac ttatcttatc
     5701 ataattcatt ttataattca acttaatttt attttgttta tttaattatt actatatata
     5761 gctatatttt tttaaaatta gttctaaaat aatttttaga tattcataca agagtgtttt
     5821 ataaaaatat ttagtgtttt atgatgaaaa ttctgctgtg taaattaaca taaaaaaact
     5881 atgaaattaa tatttataat aatttcatag tttttttatt tattgtttac tcataatata
     5941 ttctcttcat caaagcatac tctatatata caattaatat aaatttattt tataacatca
     6001 acacccatat aaggtcttaa ttcttctgga actactacag aaccatctgc ctgctgataa
     6061 ttttctaata tagcagccaa acatcttcct attgctaatc cagaaccatt taatgtatga
     6121 acatattctg cttttgattt tttatctctt ttaaatctta ttcctgctct tcttgcttgg
     6181 aaatcttcaa aatttgaaca agaagatatt tctacatatc tattgtaact tggcatccaa
     6241 acttctaaat catatttaaa tgcagctgta aatcccaaat ctcctgtaca tatttttaca
     6301 actctatatg gaagacctaa catttgtaac atagtttcag catcatgagt taatgattct
     6361 aattcattat aagaatcttc tggtttaaca aatttaacta attcaacttt attaaattga
     6421 tgttgtctaa ctaaacctct tgtatctctt cctgctgaac ctgcttcaga tctaaaacaa
     6481 ggagtatatg cacagtattt aattggaagc tctgcaacat ctaatatttc attagcatgt
     6541 atattagtaa caggaacttc agctgttggt attaagaaat actctaatcc ttctatttta
     6601 aacatatctt cttcaaattt aggaagttga ccagtcccta taaaactatt tctattggcc
     6661 ataaatggag gtaagacttc cgtatagcca tgcttagctg tatgagtatt taaaaagaaa
     6721 ttcattaaag atctttctaa tctagctcct aatcccctat ataaagtaaa ccttgaccca
     6781 gttattttcc ctgctctttc aaaatctaaa atacctagac ctgtacctat atcccaatgc
     6841 gctttatgct caaaatcaaa tttagtaggt tctccccaag ttcttatttg aacattgtct
     6901 tcatctgttt caccttgtgg tacctctgga tgaggaacat ttggtattct cattaatgta
     6961 tattccattt ttgcttctac ttcttttact ttctcatcaa taccttttat cttatctgat
     7021 aactctttca atcttgcctt ttcttctgtt acatcttttc cttcttttat taattgaggt
     7081 atttttttag aatttacatt taactcattt ttcataactt caacttcttg aagtatttct
     7141 cttcttttat tatctaattc cactacagca tcaaggtcaa attctctttc acctcttctt
     7201 tccatagctt ttttgatatc atctagattt tctcttattc ttttaatatc taacatatta
     7261 attcctccta ataattttga taatataagt tataattcca acttaattta caaacattat
     7321 acttgtaaac tcttagttta aacaaatttt aaaaataaaa aaagacctcc gtttacccta
     7381 taaaagggac gaaaatcttt tatccgcgtt gccaccctaa ttgactatac taaaatgtat
     7441 aatcctctca gacctataac gtaggtaaac gtctagcttt aactagaagc tctagagtag
     7501 attcaaaaac cattgtaatg agttttcacc atccactcat tctctgaata caatctgttt
     7561 tttactattt tccttcacag ctttttaact aattgtttta tttgataatc attatattat
     7621 atatattaac atctttcaat actattattc aaattttttt agaattttct ttaatttttt
     7681 atattaaatt cattttttct atttaattaa aaccttatct tttatccatt gtatttagat
     7741 tacctgtatt attaaataat aatgaagaag gaacaaatgg aacttttata tcattctcat
     7801 caaatttatt ttttaccttt tccattacat caaaatatac atcccagtaa tcatcagttt
     7861 ttacccaagg tctagcaact atttgcatgg tgttatttcc tatagagttt aaacctatta
     7921 taggctttgg atcatttaat attctttttt cgtttgtaaa aatttcattt aatatagact
     7981 taactacatc tatatcacta tcatatgaaa tattatatga aaaatctatt cttcttttgt
     8041 tttgatgtgt gtaattaatt atattattac ttgtcagttg aaaattaggt ataattatag
     8101 ttttattatc tggcgtctta agaacagtgc taaatatttg tatgtcagaa actgtacctt
     8161 ctacacctga accttgaata taatcaccta tattaaatgg cttaaaaaat aaaataatca
     8221 taccagagcc taaatttgat aatatttctt taaaagctaa acctatacta aatccagcag
     8281 ctcctaacac agctacaact gatgtagttt gtatccctat aatacctaag cttatcaatg
     8341 atataataga taacatagat atatatattc catatacaat aaaattattt acacctttac
     8401 tcattccata tttgattaga aactttgaaa ccatccttct tactatttta gatatataaa
     8461 tacctatcac aaatacaatt attgcatata taatatttgg tatattatca ctaaaattat
     8521 cgagcatatt ttctaaagtt ctcttagtta tattgctatt tgttgaggca gtcttagcca
     8581 atgataaaaa attcattcaa ctcctcctta tgataatatt ccgttgtcct ttaactcttt
     8641 tactatagct tctatagttt cttcttttat tgcctcaatt gtatgaatat ggctacattc
     8701 tgtcagaacc gataattgtt taaattcatt tgtctcaact ttcttcataa agttgtctat
     8761 atccatttta gaataaatat taagttctgc agttatctga ccatatgttg gatgatttac
     8821 gatgacatct tttataatac cacctaaatc cacaattatc tttaattcat cataaatttc
     8881 tttagaatcc ttatgatttt tacactttat ttgttttgta aaatacggtt gattatatat
     8941 tatatatcct tgaggtgtag ctattatttc aaatcctcta gctcttataa gggctatatc
     9001 ttgaactatt acttgcctac ttacatttaa tagttcagct agtgttccac cttttacagg
     9061 atgcttagat tcttttaaaa tatctattaa tttacttctt ctttcattag aattcatatt
     9121 acaccttctt tccaaataaa aactttacct atactatata attttatatt ttatcttatt
     9181 ataaatcaat attgacttta tattctattt aatcttaact tttataataa ataaaaaaga
//
LOCUS       DAYAPA010000146         9237 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.150, whole genome shotgun sequence.
ACCESSION   DAYAPA010000146 DAYAPA010000000
VERSION     DAYAPA010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9237
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.150"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(136..2730)
                     /gene="clpB"
                     /locus_tag="ACGI59_003340"
     CDS             complement(136..2730)
                     /gene="clpB"
                     /locus_tag="ACGI59_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFG9741108.1"
                     /translation="MDVEKMTLRVQKSLNEAYNEAVKNHNQQVDVIHLFSALINQEDG
                     LIPNIIEKMNISIDSLRNTVNFEIDKLPKVYGEGADSQGVSATRKINEVLIKAESISK
                     EFKDSYISVEHVMLAMMETESKSAVGKILKQYNINKNNFLNILSQVRGSQRVETQDPE
                     GTYDALARYGTNLVDLAKKNKLDPVIGRDEEIRRIIRILSRRTKNNPVLIGEPGVGKT
                     AIVEGLAERIVRGDVPEGLKDKIVFSLDMGALIAGAKYRGEFEERLKAVLKEVQSSDG
                     KIILFIDEIHTIVGAGKTEGSMDAGNLIKPMLARGELNCIGATTFDEYRKYIEKDKAL
                     ERRFQPVIAEEPTVEDTISILRGLKERFEIHHGVRIHDNAIVAAAKLSHRYIQDRFLP
                     DKAIDLIDEAGAMIRSEIDSLPTELDVVRRKLFTLETEREALLKENDDKSKSRLDDIQ
                     KEIAELKSKNDEMTAKYEKEKSQILDIKNLKAQLDEAKGKAEKYEREYDFNKAAEVKY
                     GEIPKLEEQIKQYEENMNDGSENSLLKEEVTEEEISSIVSKWTGIPVTKLVEGEREKL
                     LKLEDELHKRVIGQDEAVTAVSNAVIRARAGLKDERKPIGSFIFLGPTGVGKTELAKT
                     LARNLFDSEDNIIRIDMSEYMEKHAVSRLVGPPPGYVGYEEGGQLTEAVRRAPYSVIL
                     FDEIEKAHEDVFNMFLQILDDGRLTDNKGKTVDFKNTLIIMTSNIGSNYLLEAGGNIT
                     ETTNNLVMNEMKHRFKPEFLNRVDDIIMFKPLDQENIKKIIDIFMKDLKNRLKEKDIT
                     IEVTNSAKDVMVREGYDPVYGARPLKRYIGNTLETIIAKKLIAGDVYNGCTIVIDGKD
                     ENIEVLVK"
     gene            complement(2767..3363)
                     /locus_tag="ACGI59_003341"
     CDS             complement(2767..3363)
                     /locus_tag="ACGI59_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9741109.1"
                     /translation="MSLKLDRNVLQWFDYVFENEKTSLRHYNFNCTLKEISSTSLNKV
                     AFILEKNNSKYWKLYFEIPAEVTLKLKQNIHPLFREYIYEQISLYNNNQIYNFVNSNI
                     LKVFNNIAIYQYNILENLYTIDFKKSFIDKCQYLLIGEKRLIDEDLYLIAKSKEVFDF
                     FNLDGTFNLTLSFDIQKNENLLDSLLELRKSIIINERI"
     gene            complement(3380..3784)
                     /locus_tag="ACGI59_003342"
     CDS             complement(3380..3784)
                     /locus_tag="ACGI59_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="HFG9741110.1"
                     /translation="MDKTLKKDLNQEYTESKSLIMNKSKYMNVCFKNYIKVVEGMYTK
                     RDLIPLKLINSMDKNNKIRKTLYELSCEFEYPKTSLSSLFTELKKMDFLQRVKNGEYM
                     INPHIAYKGSRADKESLLAEYNQIKRPNKVSK"
     gene            4151..4525
                     /locus_tag="ACGI59_003343"
     CDS             4151..4525
                     /locus_tag="ACGI59_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFG9741111.1"
                     /translation="MNIVISNSSGKPIYEQITTQIKSMIISGELPEGSALPSMRLLAK
                     ELRISVITTKRAYSDLERDGFIETVTGKGSFVSSKNMDFVKEEQFRLIEEYLQKAVDT
                     AKSSDITYDELKDILFLLYKGE"
     gene            4522..5385
                     /locus_tag="ACGI59_003344"
     CDS             4522..5385
                     /locus_tag="ACGI59_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9741112.1"
                     /translation="MISMDAITIKNLNKTYKDFSLQDISFSVPKGSVMGFVGENGAGK
                     TTTLKAILNLISYDSGNIEIFGLDSKKNEKEIKEQIGVVFEGSNFHENLNTDHVSKIM
                     SKIYKNWNDTLFKDYLKKLRVPDNKLIKEFSKGNKMKLSIAVALSHKPKLLILDEATS
                     SLDPIVREEILDIFLDFIQDEEHSIILSSHITSDLDKIADYITFIHKGKIVFSENKDE
                     LIDTMGVLKCKPSDFDNLSREDYSYYRKSQFGYEVLLKDKHRFISRHPNCIVDNTSIE
                     EIMLFYVRGDK"
     gene            5385..6023
                     /locus_tag="ACGI59_003345"
     CDS             5385..6023
                     /locus_tag="ACGI59_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HFG9741113.1"
                     /translation="MKGLILKDLLNLKGNIKFILLFIIMFGFMSSLGDGNVNNFIGVI
                     IVLCTTMIVSTFSYDDLNKWDSYVLTMPINRNDIVLSKYLTMLIFSFIGVLVSLIVSV
                     TIGYFKNTLILNETLLINALILSISVCFGSLILPLIYKFGTERARLLMILCFLVPTLA
                     LLVFKSILENISSPISIEIILNTLVYSLPFVAILLFVISYFISSKIYSKKEV"
     gene            6515..6928
                     /locus_tag="ACGI59_003346"
     CDS             6515..6928
                     /locus_tag="ACGI59_003346"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFG9741114.1"
                     /translation="MELEITEKIEKQDEDTIFQGLLKYNLARLEDKNPVDLGIYIRDK
                     AGKIVAGFIGVTHGNWLSIKYLWVSEKLRYKGTGSQLLYKAEKIAKERGCKYVFLDTF
                     SFQAPKFYEKFGYHKVFSLENYPLTGKRYYYRKIL"
     gene            7119..8336
                     /locus_tag="ACGI59_003347"
     CDS             7119..8336
                     /locus_tag="ACGI59_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HFG9741115.1"
                     /translation="MLGKKCMDYLQTLGKISSTTNGLTRLILTQEHKKSIDLISSWME
                     GLNLDIEIDDIGNVIGTYKSSFPNAPTLVVASHQDSVKCGGIFDGMLGIIVPLVGLEE
                     AKHNNRSYPFNIKLIAFAEEEGTRFETSLMGSKVFAGTFKEELLKSVDENGITLEEAV
                     TKFGFNTKNLTNLHPRKDVDAYLEFHIEQGPVLENESLPAGIVSSITGFKSFKISVNG
                     KSGHAGTLPMNMRLDAGCCACECVLAIEKVAKTTTDLVATVGKMNFYPSSSNVVPERA
                     EFTLDVRSCSQEILDNSVEKIFNEISHICENRKLNYTSELAFENVPVPCSNKITKIIE
                     KSFIDLNLNPFYIYSGAGHDAQEMDNITDIGMVFIRCAGGVSHNPNESVSVDDLDTAV
                     KIFLKILDNLDLK"
     gene            8498..9196
                     /locus_tag="ACGI59_003348"
     CDS             8498..9196
                     /locus_tag="ACGI59_003348"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047661 - amino-acid racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HFG9741116.1"
                     /translation="MNEKLIGILAGMGPRSTAPFVDLIIDECQSQYGAKYDDEFPKMM
                     IYSLPTPFYIDRPINHDLMKETIIDGLQKLESTGVNFIAMPCNSAHIYFKELKESINI
                     PILNIVEETVKKLPTISQKVTLFSTSSTFESTIYQNGIIHNGHEFIFKDEWQIKLNNL
                     IQNIKMDKENPHNIDIWNKLIEDVKNESIENIVIACTDLNVVLEKSYPSINIVDSSKC
                     LAESVVNKYLKLVK"
BASE COUNT         3238 a         1272 c         1147 g         3580 t
ORIGIN      
        1 tgagttaaat ttaatttgga catattgact taaaaatgtt ttattaattt gataaaatat
       61 ttcttttcct atagtttata aataaaaatc agctgagcaa aatatttagt atattatgct
      121 caactgattc ttttattatt taactaaaac ttcaatattt tcatcttttc catctataac
      181 tatagtacat ccattataca catctccagc aataagtttc tttgcaatta tagtttctaa
      241 tgtatttcct atatatctct taagaggtct tgctccatat acagggtcat atccttctct
      301 caccataaca tcttttgctg aatttgtaac ttcaatagta atatcttttt cttttaatct
      361 attttttaaa tctttcataa atatatctat aattttcttg atattttctt ggtctaaagg
      421 cttaaacatt ataatatcat ctactctgtt taaaaactca ggtttaaatc tatgcttcat
      481 ttcattcatt actaaattgt tagtagtctc tgttatattt cctcctgctt ctaatagata
      541 attacttcct atattagaag tcattattat aagagtattt ttaaagtcta ctgtcttacc
      601 tttattatca gtaagtctac catcatctaa tatctgtaag aacatgttaa atacatcctc
      661 atgtgctttt tctatctcat caaatagtat tacagaataa ggagcacgtc taactgcttc
      721 tgtaagttga ccaccctctt catatcccac gtaccctgga ggaggtccaa ctagtcttga
      781 tacagcatgt ttttccatgt attcagacat atctattctt attatattat cttcactatc
      841 gaataaattt ctagcaagag tttttgcaag ttcagtttta ccaacacctg tagggcctaa
      901 gaaaataaat gaaccaattg gttttctttc atcttttaat cctgctcttg cacgtattac
      961 tgcatttgaa actgctgtaa ctgcttcatc ttgtccaata actcttttat gaagttcatc
     1021 ttctagttta agtaattttt ctctttctcc ttcaactagt ttagttactg gtatacctgt
     1081 ccactttgat acaatgctag atatttcttc ttcagtaact tcttctttta aaagagaatt
     1141 ttcagaacca tcattcatat tttcttcgta ctgctttatt tgttcttcta acttaggtat
     1201 ttcaccatat ttaacctctg ctgctttgtt gaaatcatat tctctttcat acttttcagc
     1261 tttaccttta gcttcgtcta attgagcctt taagttttta atatctaata tttgactctt
     1321 ttctttttca tattttgctg tcatttcatc atttttagat tttaactctg ctatttcttt
     1381 ttgaatatca tccaatcttg atttactttt atcatcattt tctttaagta atgcttctct
     1441 ttcagtttct aatgtaaata actttcttct aacaacatct aattctgttg gcaatgaatc
     1501 tatttcacta cgaatcatag ctccagcttc atcaattaaa tcaattgctt tatctggtaa
     1561 aaatctatct tgtatatatc tatgagaaag ttttgctgct gctacaatag cattatcatg
     1621 tattcttact ccatgatgta tctcaaatct ttcttttaaa cctctaagta tagatattgt
     1681 atcttcaaca gtaggttctt ctgcaattac aggctgaaaa cgtctttcaa gagccttatc
     1741 tttttctata tactttctat attcatcaaa agtagttgca ccaatacagt tcaattcacc
     1801 tcttgcaagc ataggtttaa taagattacc tgcatccatt gaaccttctg ttttaccagc
     1861 tccaacaata gtatgaatct catctataaa cagtataatt tttccatcag aactttgaac
     1921 ttcttttaaa actgccttta atctttcttc aaattcacct ctatattttg caccagcaat
     1981 taaagctccc atatcaagtg agaatacaat cttatctttt aaaccttctg ggacatctcc
     2041 tcttactatt ctctctgcta acccttctac aattgcagtt ttacctacac ctggctcacc
     2101 aatcaataca ggattatttt ttgttcttct tgataaaatt cttataatcc ttcttatttc
     2161 ttcatctctc ccaattacag ggtctaattt atttttcttg gctaaatcta ctaaatttgt
     2221 tccatatcta gcaagtgcat catatgtgcc ttctgggtct tgtgtttcta ctctttgact
     2281 acctctcact tgtgataaaa tatttaaaaa gttattctta tttatattat attgttttaa
     2341 tattttacca acagctgatt ttgattctgt ttccatcatt gcaagcataa catgctctac
     2401 acttatatat gaatctttaa attctttaga aatactctct gcctttataa gcacctcatt
     2461 tatttttcta gtagctgata ctccttgtga gtcagcacct tctccatata cctttggaag
     2521 cttatcaatt tcaaagttta cagtatttct caaagaatca attgatatat tcattttttc
     2581 gattatattt ggtatcaatc catcctcttg attaataagt gctgagaaaa gatgaattac
     2641 atctacttgt tggttatgat tttttactgc ttcgttataa gcctcattta aacttttttg
     2701 aactcttaaa gtcatttttt ctacgtccat ataaatccct ccattttaat attatatcca
     2761 aataaattaa attctctcat ttataattat tgattttcta agctctaaaa gactatcaag
     2821 taaattctcg tttttttgta tatcgaaact caatgttaaa ttaaatgttc catctaaatt
     2881 aaaaaaatcg aatacttctt ttgattttgc tattaaatac aagtcttcat ctataagtct
     2941 tttttcacct attaataaat actgacattt atctataaaa ctctttttaa aatcaatagt
     3001 gtatagattt tctaaaatat tatattgata aatagcaata ttattaaaca cttttaatat
     3061 gtttgaattt acaaaattgt aaatttgatt attgttatac agtgatattt gttcgtatat
     3121 atattcccta aataaaggat gaatgttttg cttaagtttt aaagtaactt cagctggtat
     3181 ttcaaaataa agcttccagt acttagagtt atttttttct aaaataaaag ctactttgtt
     3241 caaactggta gaactaatct cctttagtgt acaattaaaa ttatagtgtc tcaaactagt
     3301 tttttcattc tcaaatacat agtcaaacca ctgtagaaca tttctatcaa gttttaaact
     3361 catataaaaa ccttcttcct tatttactaa ccttattagg tctttttatc tgattatatt
     3421 ctgctaataa actttcttta tcagctctac tacctttata tgctatatgt ggatttatca
     3481 tatactcacc atttttcact ctttgtaaaa aatccatttt tttcagctcc gtaaacaatg
     3541 aagataaaga agtttttgga tattcaaatt cacaagataa ttcatataat gtttttctaa
     3601 tcttattatt tttatccata gagtttatta attttaatgg aattaaatcc ctcttagtat
     3661 acattccttc tactacttta atataattct taaaacaaac attcatatac ttacttttat
     3721 tcataattaa acttttgctc tctgtatatt cctgatttaa atcctttttt aatgtcttat
     3781 ccaaattaaa tatctcttct cactctcctt atctctaatt gataatataa atatattaca
     3841 aaaaaatatg tatgtcaaaa ctgaaaatgc aaaaagttca ccttataagt aaacttttat
     3901 ttagctataa gctaaacttt attatttttt taatttagta tttccaattt attggcagga
     3961 tttttgaaca atatttataa attttttaaa aaaatttaaa tctatttaat atggcagact
     4021 ttcataaaat acattacatt tattaaatgt ataatattgg taattagttt tttctaaaat
     4081 cagttgacat attatatata tatttatata atgtgtatat actatatata caatttaagg
     4141 aggtgtctta ttgaatatag ttattagtaa ttcgagtggg aagccaatat atgaacagat
     4201 tactacacaa attaaaagta tgattataag tggagagtta ccagaaggct cagctcttcc
     4261 aagtatgaga ctattggcaa aggaacttag aataagtgta attactacaa aacgagcata
     4321 tagcgattta gaacgagacg gatttattga gacagttact ggtaaaggca gttttgtatc
     4381 tagtaaaaac atggattttg tcaaagaaga acaatttcgt ttaattgaag aatacctaca
     4441 aaaagcagtt gatacagcaa aaagtagtga tattacatat gacgaattaa aagatatttt
     4501 atttttatta tataaaggag aatgataagt atggatgcaa taactataaa aaatttaaat
     4561 aaaacttata aagattttag cctacaagat atatcttttt cagtaccaaa agggagtgta
     4621 atgggatttg taggtgaaaa tggtgcagga aaaacaacta cattaaaagc aattttaaat
     4681 ttaatatctt atgatagtgg aaatatagaa atctttggtc ttgatagtaa aaaaaatgaa
     4741 aaagaaatta aagagcaaat tggggttgta tttgagggaa gcaactttca tgaaaattta
     4801 aacactgacc atgtttcaaa aatcatgagt aaaatctata aaaattggaa tgacacatta
     4861 tttaaagact acttaaaaaa attgagagta ccagacaata aattaattaa ggagttttca
     4921 aaggggaata agatgaaact tagtattgct gtagccttat ctcataaacc aaaattatta
     4981 atactagatg aggcaactag ttcacttgac cctatcgttc gtgaagaaat actagatata
     5041 tttttagatt ttatacaaga tgaagaacac tctattatct tatcttcaca tattacaagt
     5101 gatttagata agatagcaga ctatattaca tttatacata aaggaaaaat tgtatttagt
     5161 gaaaataagg atgaattaat tgatactatg ggagttttaa aatgtaaacc tagtgatttt
     5221 gataaccttt caagagagga ttattcatat tatcgtaaaa gccaatttgg atatgaagta
     5281 cttcttaaag ataagcatag atttatctca agacatccaa actgtattgt tgataatacg
     5341 tctatagaag aaattatgtt attttatgtg agaggtgata aataatgaaa ggtctaattt
     5401 taaaagattt acttaatttg aaaggaaata taaagtttat acttttattc atcattatgt
     5461 ttggctttat gtcttcttta ggagatggaa atgttaataa ctttataggt gttattatag
     5521 ttttatgtac tacaatgatt gtttcaacat tttcatatga tgatttgaat aaatgggata
     5581 gttatgtact tacaatgcct attaatcgca atgatattgt attaagtaaa tatttaacta
     5641 tgttaatatt tagctttata ggtgttcttg tttcacttat tgttagtgta actattggtt
     5701 attttaaaaa tacacttata ttaaatgaaa cactacttat taatgcacta atcttatcaa
     5761 tttcagtatg ttttggtagc cttattctac cacttattta taaatttggt acagaaagag
     5821 ctcgtttatt aatgatttta tgctttttag ttccaacact agctttatta gtttttaaaa
     5881 gtattttaga aaatattagt tcacctattt ctatagaaat tatacttaat acactagtat
     5941 attctcttcc atttgttgct attttactat ttgtgatttc atattttata tcatcaaaaa
     6001 tctatagtaa aaaagaagta taaaatatac tatatatgtt tctgtattta agtatttaaa
     6061 taaaatacta tcgagtttat tttggtagta ttttattttt taattattaa tctctactag
     6121 gtatttatta ttttattact tatatttaat aagactattt actactttct aatcttattt
     6181 atataattta ttcattttat aaattgaact actaatgata ttattgaaca tagcaataga
     6241 ataacatcta aaatagtttt tatgtattaa attaacttat tttaatattc aacaatttag
     6301 gtttactaaa tttattgatg atttcttttt atataactta aactacattt ttatttaaaa
     6361 aaatttaata tcaacaatat aaaattaaga ataagtatta ttagtttgac attagcataa
     6421 atgcctatta gaataatgtt aaactatatt ctttctactg attttattaa aaaataccaa
     6481 tatacaaaag attatacaag gagaaagttc aaatatggaa ttagaaatta cagaaaaaat
     6541 agaaaaacaa gatgaagata ctatttttca aggattatta aaatataatc ttgctagact
     6601 tgaagataaa aacccagtgg atttaggaat atatattcga gataaagcag gtaaaattgt
     6661 tgcaggtttt ataggtgtta ctcatggtaa ttggctttct ataaaatact tatgggttag
     6721 tgagaagctt agatataaag gtactggaag tcaactttta tataaggctg aaaaaattgc
     6781 taaagaacga ggttgtaaat atgttttttt agatacattc agttttcaag cacctaaatt
     6841 ttatgagaaa tttggatacc ataaagtatt ttcacttgaa aattatcctc taactggaaa
     6901 gagatattat tatagaaaaa tactataata ataaaaatca tatttcatat ttttaaatta
     6961 aaacttctgc tcttcgtcta cagactttat gtttttatat cattattaat actaaaatag
     7021 tattttacta tacatacaaa attatttgaa agttcaaaaa ttataagtat aaaagctaat
     7081 cttagcttaa ataaaaatta tatttttagg aggtaaatat gttaggaaaa aaatgtatgg
     7141 attatctaca aacattagga aaaataagtt caacaacaaa tggtcttaca agacttatct
     7201 tgactcaaga acataaaaaa tctattgatt taattagttc atggatggaa ggtttaaatc
     7261 ttgatattga aattgatgac attggaaacg ttataggtac atataaatca agctttccaa
     7321 atgctcccac attagtagtt gcatctcatc aagatagtgt aaagtgtgga ggtatatttg
     7381 atggtatgtt aggtataata gtgcctctag ttggtcttga agaagcaaaa cacaataata
     7441 ggtcttatcc tttcaatatt aagctaattg ctttcgctga agaggaagga actagatttg
     7501 aaacttcttt aatgggaagt aaagtttttg ctggtacttt taaagaggaa cttcttaaat
     7561 ctgttgatga aaatggaatt acacttgaag aagctgtaac aaagtttgga tttaatacaa
     7621 aaaacttaac aaatttgcat cctagaaaag atgtagatgc atatttagaa tttcatattg
     7681 agcagggtcc tgttttagaa aatgaatctc ttcctgctgg aattgtatca agtattacag
     7741 gatttaaaag ttttaagata tcagtaaatg gtaagtctgg acatgctggt acacttccaa
     7801 tgaatatgag acttgatgct ggatgttgtg cctgtgaatg tgtattagct atcgaaaaag
     7861 tagctaaaac gactactgat ttggttgcta ctgtaggaaa gatgaatttc tatccatctt
     7921 catcaaatgt ggtacctgaa agagctgaat ttacactcga tgtacgctct tgttcacaag
     7981 aaattcttga taattctgta gaaaagatat ttaatgaaat atcacatata tgtgaaaata
     8041 gaaaacttaa ttatacaagt gaacttgctt ttgaaaatgt tcctgtacca tgttctaata
     8101 aaataacaaa aatcatagaa aaaagtttta tagatttaaa tcttaatcct ttttatattt
     8161 atagtggtgc tggccatgat gctcaggaaa tggataatat aactgatatt ggtatggtat
     8221 ttataagatg cgctgggggc gtaagtcata atcctaatga aagtgtctct gtagatgact
     8281 tggatactgc tgtaaaaata tttttaaaaa tactagataa tcttgattta aaataattct
     8341 tatataagaa agtatattaa aatatataat ctaaaaaatc actttatatt tatattatat
     8401 aagtgtacaa tataatctat tataaaatat aattttatgc ttttatagat ttttatttta
     8461 tacataaata tatttatata taaaggagat aatttttatg aatgaaaaat taattggtat
     8521 tttggctgga atgggtccaa ggtcaacagc cccttttgtt gatttaatta ttgatgagtg
     8581 tcaatctcaa tatggagcaa aatatgatga tgaatttcca aagatgatga tatattcatt
     8641 acctacccca ttttatattg accgtccaat aaatcatgac cttatgaaag aaactataat
     8701 tgatggatta caaaagcttg aatcaactgg tgtaaatttt attgcaatgc catgcaattc
     8761 agcacatata tactttaaag aattaaaaga gtctattaat ataccaatat taaatattgt
     8821 agaagaaaca gttaaaaaat tacctactat ctcacaaaaa gtaactcttt tttcgactag
     8881 ttcaactttt gagtctacaa tttaccagaa tggtataata cataatggac atgaatttat
     8941 atttaaagat gaatggcaaa taaaattaaa caatcttatt caaaatataa aaatggataa
     9001 agaaaatcca cacaatattg atatttggaa taaacttatt gaagatgtta aaaatgaatc
     9061 tatagaaaat atagtaattg cttgtactga tttaaatgta gttttagaaa aatcatatcc
     9121 ttcaataaat attgttgatt catcaaaatg cttggctgaa tcagttgtca ataaatatct
     9181 aaaattagtt aaataaattt ttcatacact atattataaa aaatatatgt tataagt
//
LOCUS       DAYAPA010000147         9074 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.151, whole genome shotgun sequence.
ACCESSION   DAYAPA010000147 DAYAPA010000000
VERSION     DAYAPA010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9074
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.151"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(84..926)
                     /gene="licT"
                     /locus_tag="ACGI59_003349"
     CDS             complement(84..926)
                     /gene="licT"
                     /locus_tag="ACGI59_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HFG9741117.1"
                     /translation="MIIQQIYNNNVVLVLDENIKKELILTGCGIGFQKKKGQEIDKSK
                     IERTFVIQDESFLDKISKLASQVDEKFFEVSTEIIAYAEENLNTKLYEYIYVALTDHI
                     AFAIKRYHENITIKNDLLHEIKRIHKKEYEIGKWAVDYINKEFDVKFPVDEAGFIAMH
                     IVNSNYKGSSKESLLITKIVKDILNIIRYYYRVEFKEDDINYDRLLTHLKFFAKRLVK
                     KEKINDTNNEIIDIIKVKYEKDYDCAYKIKTHVEKNYDYYVSQDELLYLTLHIKRVIS
                     VLNL"
     gene            complement(1187..3229)
                     /locus_tag="ACGI59_003350"
     CDS             complement(1187..3229)
                     /locus_tag="ACGI59_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFG9741118.1"
                     /translation="MSILVTLLGLLVCTTISTVFSKKWSNIPLAIYQIVLGIILSILP
                     FKFSFSFNPEIFVICIIAPLLFSEGQNVSRKELLELRKPILLLAFGLVLITVFAGGIF
                     IHFLIPGMPLSVSFALAAVISPTDLVAVKSITQGLNFPKNMMSILEGESLLNDAAGVV
                     AFKVAVLATVTGVFSIEEAGIQFMITAFGGIIVGSILGYIIIKIRLSLHKWNLEEIPM
                     VIVIQIMTPLFVYFVAEEIGVSGILAVVMAGIAHGIEKEHLQNTTTKLRIISDNTWYV
                     LEYVLNGFVFTLLGFLLPSIYTGLSSKNENMALELTFISTLIVLILFVIRFIWVYLWH
                     NSFIKPKKNPLNNFFVGFLGFKDEEVVKESISKCKYSLIVATCGVHGTFTLATALSIP
                     FYLADKDVFPMRDTVLFISSEVILISLVLATVLLPRLLKNNVQESEPLLSDKEAYKLI
                     LKEAILKLSNEKAQEVQPVIHDLNEQLVDSEKGILNTPDNKLISMMIAEADQQGLLAV
                     LKLLEDGKISDRAFKLYRFYITKSRKSVQKSIFKIVKLKISMWIINRKVKKEVKEKIT
                     KIVSENKEIIKEFHHAYALSTKTAISFINKNTDEDNRHEALIAIRFYNRYLNWITRKI
                     QNDDKFEARVEYYRVMAIQSQRDSVQQLVETKQISREVAKELLENILYDEMMMIES"
     gene            complement(3381..4352)
                     /locus_tag="ACGI59_003351"
     CDS             complement(3381..4352)
                     /locus_tag="ACGI59_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9741119.1"
                     /translation="MKKEESLLKVEGLKKYFEVKSGLFDKEIKCVKAVDGISFDLKKG
                     ETLAIVGESGCGKSTAGRTILRLIEKTEGKVEFNGVDIYKLEKEELRKLRPKMQMIFQ
                     DPYSSLNPRLNVEQIISEAVIEHNLVPKNEIKDNILNVVESCGLSKYHLNRKPGEFSG
                     GQRQRIGIARALALNPEFIVCDEPVSALDVSIQSQIINLLMDLQDKYKLSYLFISHDL
                     SVVNHIAHRVCVMYLGSIIEIATKGELYLHSQHPYTKALMSAVPIQDPTIKKNRIILK
                     GDIPSPTNPPSGCKFHTRCPYCKEICKKEIPVLKEISRNHFVACHLV"
     gene            complement(4353..5330)
                     /locus_tag="ACGI59_003352"
     CDS             complement(4353..5330)
                     /locus_tag="ACGI59_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9741120.1"
                     /translation="MSKALIEVKDLKVYFHTDKGIVKSVNEVSFNINEGETIGIVGES
                     GCGKSVTAMSLMKLLPTSKIEGGEIIFRGKDILKMNEDELMGIRGNEISMIFQEPMTS
                     LNPAFTIGSQIIEGIMIHQDLSKEEAKKKVIDMIKLVEIPRAEEIYNSYPHELSGGMR
                     QRIMIAMALSCNPKLLIADEPTTALDVTIQAQILDIMKNIKEKLNTSIMMITHDLGVV
                     AEMCDKVLVMYAGKIIEVAEVVELFKNPKHPYTIGLLKSKPALGKNKDKRLYSIPGQV
                     PNPIGMPDSCYFSDRCEKVCDKCRTQIPPLIELNSGHSIACWLYKKEEI"
     gene            5549..7108
                     /locus_tag="ACGI59_003353"
     CDS             5549..7108
                     /locus_tag="ACGI59_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFG9741121.1"
                     /translation="MKFKKLASLILVSSLMLTFTACASNDKDKSSGSKSGGTVVIPMA
                     SDPDIINGAFANVKEDSLASNIVYAPLYTYEKGNLVNYLAEKVDFKDSKELTIKLKSN
                     LKWHDGKPITAEDVLFTFNTVLDEKQNSPSRQYLLVGEKPVKVEKIDDLTVKITLPTA
                     SESFLYGISKISPIPKHVFEGESNIAKSEKNNNPVGSGAFKFKEWKKGESIVFEKNAD
                     YFGGESKADSIALKIIPNEASQEAALNNGEISLMKTSAEGYEKAKSNSNLQTYTYSEE
                     RLNYIVFNQNISNMANKEVRQALSYALNRNEMIESAYGKEGSVPAKSILVPEADFYTE
                     EGVEGYDQDTNKAKDLLDKSGVKIDKLKIGYNTGRFGHKNYALVAQQELKKIGIEAEI
                     VPYESKAFFNILFSNSTECDMYVNGYAWGLEPNPYRGMFETGQYCNQTKYSNAEIDAL
                     WEKGFTELNKEKREEIYKQIQQDISKDAPIYTIDYEQNLMAAQKNLKGIKDAKPSPAI
                     LFEDWSKLYVE"
     gene            7214..8173
                     /locus_tag="ACGI59_003354"
     CDS             7214..8173
                     /locus_tag="ACGI59_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9741122.1"
                     /translation="MLKLILNRVKVLIPMLILISILSFGLLELAPGDPADAYINPLMS
                     SQDIENIRVNMGLDKPAYIRYLNWLKNTLSGNLGISYINHMPVTEQIMEKMGNTFILM
                     GTSLIFSILVAIPLGIFLAVNKNSITSKVSSVFNYIGVSIPSFWIGMILISIFSVKLN
                     IFPSGGMHTIGNDSIGDLAKHLVLPVITLGLYNTAIFTNYVEASVNEQLKKQYVITAR
                     AKGLSEKVILFKHVLKNSLTSLVTILGMSIQKLVTGAFVTEVVFSWPGMGRLMIDSIF
                     SRDYTVIMAITMLSALFLILGNLVADILYLLIDPKIKSSKGGF"
     gene            8176..8988
                     /locus_tag="ACGI59_003355"
     CDS             8176..8988
                     /locus_tag="ACGI59_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9741123.1"
                     /translation="MFTSILKKLKKDKSFAFACITLSIIIILIIASFILNIDSNAIDT
                     TSKLQEPSLKHIFGTDELGRDYFSRALYGGRISLSVGILSMLISVVFGTAVGVISGYI
                     GGKLDQFLMRLVDMLMSIPSFLVLIVLNTYLTPKVSTIILIIGFLSWMEVSRIVRGET
                     LKIKENEYCLAAKATGIKTRNILLRHIVLNLKETIVVAGSLNIANAILTESALSFLGL
                     GIQLPLASWGNMLQDAQRHIFDKLYLAVFPGLLIFLTVMSLTIISKKFSSNT"
BASE COUNT         3144 a         1379 c         1132 g         3419 t
ORIGIN      
        1 tattttatat taaattttaa ataatatatt aaaattaaac ataatatatt aaatctatac
       61 attctgtatt aaactactct ttattataaa ttcagtacag atataactct tttgatatgt
      121 aatgttaaat aaagtagttc atcttgactt acatagtaat cataattctt ttctacatga
      181 gtttttattt tatatgcaca atcataatcc ttttcatact ttactttaat tatatctata
      241 atttcattat ttgtatcatt gattttttct ttttttacta atcgttttgc aaagaatttt
      301 aaatgagtca gaagcctatc ataatttata tcatcttctt taaattcaac tctataataa
      361 tatcttataa tatttagtat gtcttttaca attttagtaa ttaacagtga ttcctttgaa
      421 cttcccttat agtttgaatt tactatatgc attgctataa aaccagcttc atctactgga
      481 aatttcacat caaattcttt atttatataa tctacagccc atttcccaat ctcatattct
      541 tttttatgta ttctttttat ctcatgtagt aaatcattct ttatagttat gttttcatga
      601 tatcttttaa ttgcaaatgc aatatggtca gtaagtgcta cataaatata ttcataaagt
      661 ttagtattca aattctcctc tgcataagca attatttcag tactaacctc aaaaaatttc
      721 tcatcaacct ggcttgctag tttactaatt ttatctaaaa agctttcatc ttgtattaca
      781 aaagttcttt caattttact tttatctatt tcttgacctt tctttttttg aaacccaatt
      841 ccacatcctg ttaatattaa ttcttttttt atattttcat ctaaaactaa tacaacatta
      901 ttattataaa tttgttgaat aatcataatc aatatctctc ccaaaataca atttccctta
      961 ttcataaaaa aatacctaaa ttaagataca attcacctgt ataccttaac ctaggtattg
     1021 cctgcttacc agtaacaatc cagcttgtta acgcttatat attgtattca atttatggtt
     1081 ttattctatt ataaataaat atatatgtca agtaaattat tgaaaactat ttcctatttt
     1141 ttagtattaa ttttaacatc aaaaatcaaa ttttattcca ttatatttat gattctatca
     1201 tcatcatttc atcatagaga atattttcca ataactcttt agccacttct cttgatattt
     1261 gcttagtttc aactaattgt tgaacactgt ctctttgact ttgtatcgcc attactctgt
     1321 agtattctac tcttgcctca aacttatcat cattttgaat ctttctagta atccaattca
     1381 aataacgatt atagaatctg attgctatta aggcctcatg tctgttatct tcatcagtat
     1441 ttttatttat aaatgatata gcagttttag tagaaagtgc atatgcatgg tgaaactctt
     1501 tgattatttc tttattctca gatactattt ttgtaatttt ttcttttact tctttcttta
     1561 cttttctatt tataatccac atgcttattt ttaattttac aattttaaat atagattttt
     1621 gaacagattt tcttgatttt gtaatataaa atctatacaa tttaaaggct ctatcagaaa
     1681 ttttaccatc ttctaatagt ttcaaaacag ctaataatcc ctgttggtct gcttctgcaa
     1741 tcatcatact tataagttta ttatctggag tatttagtat tcctttttct gagtctacca
     1801 actgttcatt taaatcatgt ataactggtt gtacttcttg tgctttttca ttactaagtt
     1861 ttaaaatggc ttctttcaaa attagtttat atgcctcttt atcagataat aaaggttctg
     1921 actcttgtac gttatttttt aataatcttg gcagtaaaac tgttgccaat actaaactta
     1981 taagaataac ttctgatgat ataaataaaa cagtatctct cattggaaat acatctttat
     2041 cagccaaata aaaaggtata gataaggcag ttgctagtgt aaatgtacca tgaacaccac
     2101 aagtagcaac tattagtgag tatttacatt tagaaatact ctcttttaca acttcttcat
     2161 ccttaaaacc taaaaaacct acaaaaaaat tatttaaagg attcttttta ggttttataa
     2221 aagaattatg ccataaatat acccatataa atcttataac aaataaaata agtacaatta
     2281 aagtagaaat aaaagtcaat tccaatgcca tattttcatt tttactacta agtcctgtat
     2341 atatacttgg taacaaaaaa ccaagcagtg taaagacaaa tccattcaat acatactcca
     2401 aaacatacca agtattgtct gatattatcc ttaatttagt agttgtattt tgtaaatgct
     2461 ctttttcaat tccatgagct atccctgcca ttacaacagc taggattcca gatactccta
     2521 tttcttctgc tacaaaatat acaaaaagtg gagtcattat ttgtataact attaccattg
     2581 gaatttcttc caaattccac ttgtgtaaac taagtcttat ttttataatt atgtatccta
     2641 aaatacttcc tactattatt cctccaaaag ctgtaatcat aaattgaatc cctgcctctt
     2701 ctattgaaaa aactcctgta actgtggcaa gtactgcaac cttaaatgct actactccag
     2761 cagcatcatt taataaagat tcaccttcaa gtatactcat catattttta ggaaaattta
     2821 atccttgtgt aattgatttt actgctacta aatcagttgg agatattact gctgccaaag
     2881 caaatgaaac agataatggc atcccaggaa taagaaaatg tataaagatt ccacctgcaa
     2941 acacggttat taaaactaaa ccaaatgcaa gaagcaaaat tggttttctg agctctaata
     3001 gctcttttct cgatacattt tgtccttcac tgaatagtaa tggagctatg atacatatta
     3061 caaatatttc tggattaaat gaaaatgaga atttaaatgg caaaatagac aatataatcc
     3121 ctagtacaat ttgatatatt gcaagaggaa tattactcca ctttttgcta aatacagttg
     3181 aaatcgttgt acaaaccaat aatcccaaca atgttactaa aattgacata actacctctc
     3241 ttttcaataa taatattata gattaaactt atatatcaac taacttaata ttaacagcat
     3301 gaaatcccat cttaatcaaa aaaattttca gtttattcta ttattcttat tatatacatc
     3361 aaatttaatt tttgaaatat ctatactaga tgacatgcaa caaaatgatt tcttgatatt
     3421 tcttttaaaa ctggaatctc ttttttgcat atttccttac aataagggca tcttgtatga
     3481 aacttacatc cacttggtgg atttgttgga gatggtatgt ctccttttaa tataattcta
     3541 tttttcttaa ttgtagggtc ttgtattggt actgcgctca taagcgcttt tgtatatgga
     3601 tgttgagaat gtaagtaaag ttctccttta gttgcaattt ctattataga acctagatac
     3661 ataacacaaa ctctatgtgc aatatgattt actacactca aatcatgaga tataaataaa
     3721 tatgaaagtt tatatttatc ctgtaaatcc ataagtaaat ttataatttg agattgaata
     3781 gaaacatcta gtgcagaaac aggttcatca caaactataa attctggatt taatgctaat
     3841 gctcttgcta tacctattct ctgtctttga cctccagaaa actcacctgg ttttctattt
     3901 aaatggtact tagaaagacc acaactttct acaacattta atatattatc ttttatttca
     3961 ttctttggca ctaaattatg ttctataact gcctcggata ttatttgttc cacatttaat
     4021 cttggattta atgaactata tgggtcttga aatatcattt gcattttggg tcttaacttt
     4081 ctaagttctt ccttctctaa cttatatata tctacaccat taaattcaac tttaccttca
     4141 gttttttcaa ttaatctaag aatggttctt cctgcagttg attttccaca tcccgattct
     4201 ccaactatag ccaaagtttc tccttttttt aaatcaaaag aaattccatc tactgccttt
     4261 acacatttaa tttccttatc aaataatcca gatttaactt caaagtactt tttaagacct
     4321 tctactttta ataaactctc ttcttttttc aattatatct cctccttttt atataaccaa
     4381 catgcaatac tatgaccact attcaattct attaaaggag gtatctgagt tctacattta
     4441 tcacatactt tttcacatct atcactaaaa taacatgaat ctggcatccc aattggattt
     4501 ggtacttgtc ctggaatcga atacaatctt ttatctttgt tttttccaag agcaggcttt
     4561 gattttagaa gtccaatagt ataaggatgt tttggatttt taaataattc aacaacctct
     4621 gccacttcta taattttgcc agcatacatc actaatactt tgtcacacat ttctgcaact
     4681 actcccaaat catgtgtaat catcattata gatgtgttta atttttcttt tatatttttc
     4741 attatatcta aaatctgagc ttgaatagta acatctaaag ctgttgttgg ttcatcagct
     4801 ataagtaatt ttggattaca tgataaagcc atagctatca ttattcgttg tctcattcca
     4861 cctgataatt catgtggata tgaattatat atttcttcag ctcttggtat ttctaccagt
     4921 tttatcatat cgattacctt tttcttagct tcttcctttg ataaatcttg atgtatcata
     4981 ataccttcaa tgatttgact tccaatagta aacgctgggt ttaaagatgt cattggttct
     5041 tgaaaaatca ttgatatctc attccctctt attcccataa gttcatcttc attcatcttt
     5101 aaaatatctt tgcctcgaaa gattatttct ccaccttcta tttttgatgt tggaagtaac
     5161 ttcatcaaag acatggctgt tacagactta ccacatcctg attctccaac tattccaata
     5221 gtttcacctt catttatatt aaaacttact tcatttacac tctttacaat ccctttatct
     5281 gtatgaaaat aaacttttaa atctttaacc tcaattagtg ctttactcat tattttctcc
     5341 tctcagcaaa attacttaag ataacataca ccttttgatt gtattttata aaaatttaaa
     5401 ttacaacatt ttcttacatt ttattatttt tttctattaa ttataataat ataatagtta
     5461 atttctatat atattgattt aataatgatt actattatac aataaaaaat atgtgttaaa
     5521 tattatttta tggaggtttt ataagaatat gaaatttaaa aaattggcat cattaatctt
     5581 agtttcatca ttaatgctta catttacagc ctgtgcttcc aatgataaag acaagtctag
     5641 tggaagcaaa tctggtggaa ctgttgtaat acctatggca agtgacccag atataatcaa
     5701 tggagctttt gcaaacgtta aagaagactc tcttgcatca aatatagttt atgctcctct
     5761 ttatacttat gaaaaaggaa atttagtaaa ttatttagca gaaaaagtag attttaaaga
     5821 tagtaaggaa ttaactataa aattaaaatc caatttaaaa tggcatgatg gaaagccaat
     5881 aactgctgaa gatgtcttat ttacatttaa tacagtttta gacgaaaaac aaaattcacc
     5941 atcaagacaa tacttattgg tgggtgagaa accagttaaa gtagaaaaaa ttgatgattt
     6001 aacagttaag attactttac caactgcaag tgaatctttc ttatatggta tatctaaaat
     6061 ttctccaata ccaaaacatg tgtttgaagg agaatcaaat atagctaagt cagaaaaaaa
     6121 taataatcca gttggttctg gtgcattcaa gtttaaggaa tggaaaaaag gtgaaagcat
     6181 agtatttgaa aagaatgcag attattttgg tggagaatct aaggcagatt ctatagcgct
     6241 aaaaataatt cctaatgaag catctcaaga agcagcttta aataatggcg aaatttcact
     6301 tatgaagaca tctgctgaag gatatgaaaa ggcaaaatca aactcaaatt tacaaactta
     6361 tacttatagt gaagaaagat taaactacat tgtatttaac caaaatatat caaacatggc
     6421 taacaaagaa gtaagacaag ccttatcata tgccttaaat agaaatgaaa tgatagaatc
     6481 agcatatgga aaagaaggtt ctgtacctgc taaatctata ttagttccag aagcagactt
     6541 ctatacagaa gaaggtgttg aaggatatga ccaagataca aataaggcta aagacttatt
     6601 ggataaaagt ggtgttaaaa ttgataaatt aaaaataggt tataacacag gtagatttgg
     6661 gcataaaaac tatgccttag tagctcaaca agagttgaaa aaaattggta tagaagctga
     6721 aatagttcct tatgaaagta aggcattctt caatatacta tttagtaata gcactgaatg
     6781 tgatatgtat gtaaatggtt atgcttgggg attagaacca aacccatata gaggtatgtt
     6841 tgaaactggt caatattgca accaaactaa atattctaat gctgaaatag atgctttatg
     6901 ggaaaaagga tttactgagt taaacaagga aaaacgtgaa gaaatatata agcaaataca
     6961 acaggatata tctaaggatg cgcctattta cactatagat tatgaacaaa atttaatggc
     7021 agctcaaaaa aatctaaaag gaataaaaga tgctaaacca tctcctgcaa tattatttga
     7081 agattggtct aaactgtatg tagaataatt gatgtaaaat tttatattat taattagaaa
     7141 aggagatgtc taaaaataat attgttttat tattttaaaa cgagacatct cttttaatta
     7201 ggagaattta cttatgttaa aattaatatt gaatagagta aaagttttaa tacctatgtt
     7261 aatcttaata tctatattat catttggact tttagaatta gctcctggtg acccagcaga
     7321 tgcatatata aatcctttaa tgtcttcaca ggatattgaa aatataaggg ttaatatggg
     7381 gcttgataaa ccagcatata taagatattt aaactggttg aaaaacactt taagtggaaa
     7441 cttaggtatt tcttatataa accatatgcc cgttacagaa caaattatgg aaaaaatggg
     7501 aaatacattt attcttatgg gtacttcttt gatattttca atattggtag ccatacctct
     7561 tggaatattt ttagctgtaa ataaaaattc aataactagt aaagtatcat ctgtttttaa
     7621 ttatataggt gtttctatac cttctttttg gataggtatg atactcattt caattttttc
     7681 agttaaactt aacattttcc catcaggagg tatgcatact attggaaatg atagtattgg
     7741 agatttagca aaacatttag ttttgcctgt aataacttta ggtctatata atactgctat
     7801 ttttacaaat tatgtagaag ctagtgtaaa tgaacaatta aaaaaacaat atgttattac
     7861 agcaagagca aaaggattat cagaaaaagt catactattt aaacatgtct tgaaaaattc
     7921 actgacatca ttggttacaa tcctaggcat gagtatacaa aaattagtca ctggtgcatt
     7981 tgttacagaa gtagttttct cttggccagg tatgggaaga ttaatgattg attcaatttt
     8041 ttcaagagat tatactgtaa ttatggcaat aactatgtta tcagcactat ttttaatttt
     8101 aggtaattta gttgctgata tactatactt attaattgac cctaaaataa aatctagcaa
     8161 aggaggattc tagttgtgtt tacaagtatc ttaaaaaaat taaaaaaaga taaatccttt
     8221 gcttttgcat gtattactct tagcataata atcatattaa ttatagcttc ttttatatta
     8281 aatatagact ctaatgcaat tgatactact tctaaacttc aagaacctag cttaaagcat
     8341 atctttggta cagacgaact tggtagagat tacttctcta gagctcttta tggtggaagg
     8401 atttcactta gtgttggtat tttatcaatg cttatttctg tagtatttgg tactgctgta
     8461 ggtgttatta gtggatatat tggaggtaag ctagaccaat tcttgatgag attagtggat
     8521 atgcttatga gtataccaag tttcttagtt ttgattgtat taaatactta tctaacacct
     8581 aaagtttcca ctattatact aattattgga ttcttaagtt ggatggaagt ttcaagaatt
     8641 gtacgtggcg aaacattaaa aataaaggaa aatgaatact gtctagctgc taaagctact
     8701 ggtatcaaaa ctagaaatat actattaaga catatagttt taaacttaaa agagactatt
     8761 gtagttgctg gttcgttaaa tattgctaat gctatcttaa ctgaatctgc tcttagtttt
     8821 ttaggtcttg gaatccaatt gcctcttgca tcatggggta atatgcttca agatgctcaa
     8881 agacatattt ttgacaagtt atatttagct gtatttccag gattacttat atttttaaca
     8941 gtcatgagtt tgactataat aagcaaaaaa ttttctagta atacttaaca aattttatat
     9001 agaaaataaa aacagtgatt atctaaagat atacactgtt tttatttttc gtttaaaaaa
     9061 atacatattt tgta
//
LOCUS       DAYAPA010000148         8953 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.152, whole genome shotgun sequence.
ACCESSION   DAYAPA010000148 DAYAPA010000000
VERSION     DAYAPA010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8953
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.152"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(25..633)
                     /locus_tag="ACGI59_003356"
     CDS             complement(25..633)
                     /locus_tag="ACGI59_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HFG9741124.1"
                     /translation="MEKDFFRKNGIDLAKSILGKYLIRKYENKVIVTKIIETEAYMGV
                     NDKGAHVYGNKKTDRTKPLYLDGGHIYVYLIYGMYNCLNLSANIENVPECVLIRGVEP
                     ITSLDEISMNRYNKAYTELSKYQVKNITNGPGKLCKALKIDRSLNSKSIMGEELYISD
                     FYYDDKGKKVFSKDELDIKTSKRINIDYAEEAKDFLWRFYIE"
     gene            complement(742..3480)
                     /locus_tag="ACGI59_003357"
     CDS             complement(742..3480)
                     /locus_tag="ACGI59_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFG9741125.1"
                     /translation="MESKGTLSKNKVYILANGLFLVIALLNINLYTVSINMYRIILLW
                     IFGYSIVYISSFKTDEFINILKVGCLVLVFVSILGVVNLKIQKFQIVLLNLSIINMST
                     FIYLTISKVKKLRISPILIFYSAFVILDYFIRNIFSDINWNMIIILINICISMFNLLY
                     LLNKLDSNIEHYKLIKDLTYFVFFTTIICFVAISKINIVDSDRLIGVIYLLLCNHTYY
                     VYVYSLNNRVVYPYYELDSINKKLSKKSEQLGKINLAIEKDMIIQRTLKNYIDQRKEL
                     LRQALDTIPNVWIVTDYDFNISYTNNKFKDEFSKDMNNYYKILTFIKEDEKVAEKLKK
                     FDNDISIIDKLVELNDKIYLLSLSNNKDESNYLISLNDITNEIKIDEEIRNINKDYEN
                     IILNIPSPILVRSAEGGIKKNKVISINKKYEKSFKCVSSDLVDMTLEQYFETFNIDFF
                     DNREFKRLNLTQEKKAEIVSYNDTANCIVNFVMSDSEGEEHVEEVRVGDYWSDNKLFK
                     LLTFRDITKEINILRTVNEQKIIYEKLLDVIPEAIFLEDLSTSRVLYTNRAFRELFGI
                     SSDVLGVTTQKYRNILVKKYINNLNIGEREKSIHIVNENNHIKEVKMISRTLYFGQKR
                     SRVRIIKDLSVQRESERLKKALIKQRQYDQMKMEFYANISHELKTPLNNIYSSVQLIE
                     NLYKKGKIIDFQDILKEHIKITKQNMFRLLRLIDNIINISQVKSDIYKIKAVNFDIID
                     ITERIVTSISSYAKSKGIDLIFDTDEETVMVGLDPESIERIILNILSNAIKFTLEGGE
                     ILVGIYKKDETVEIIIKDTGVGIDKEKLKDIFNRFKQIENSGISNEFGSGIGLCLTKS
                     LIEIQNGKIYIDSKVGEGTNVKVIFPIKEVVEEVYDNSNYNDNIEKFEIEFFDIYK"
     gene            complement(3628..4419)
                     /locus_tag="ACGI59_003358"
     CDS             complement(3628..4419)
                     /locus_tag="ACGI59_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFG9741126.1"
                     /translation="MKLKKLLSVALVSAIAISAVGCSNKEDKKILVGASSNPHAKILE
                     VAKPLLKEKGYDLEVKIFDDYVLPNTALDEGSLDANFFQHIPFLEETVKEKGYKLTYT
                     SKVHIEPMGFYSEKVKSLDELKDGAVIAVPNDATNGARALKLLAKNKLIEVKDGELIT
                     KKDITKNPKNIVIKEMNAEQLPTVLKDVDGAVINSNYALTANLNPTKDAIVIESSDSP
                     YVNIIACRENNKDSDKIKALSEAMNSKEVKKFIQDEYKGSIVPAF"
     gene            complement(4455..5111)
                     /locus_tag="ACGI59_003359"
     CDS             complement(4455..5111)
                     /locus_tag="ACGI59_003359"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFG9741127.1"
                     /translation="MNSLIDFLTTLFPNALLQTLYMVIVPTIVATILGFILAIILVVT
                     KPDGLKPNSTINSALGFIVNIFRSFPFMILIVAMIPITRLIVGTSIGETAAIVPITIG
                     AAPFIARIIESSLNEVDKGLIEAAKSFGATKRQIVFKVMIKEAMPSIVSGITLSIISI
                     LGYTAMAGAVGAGGLGNIALIYGYQRFDTAVMVYTVIALIILVQIIQGVGNLAYKKLK
                     "
     gene            complement(5104..6069)
                     /locus_tag="ACGI59_003360"
     CDS             complement(5104..6069)
                     /locus_tag="ACGI59_003360"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFG9741128.1"
                     /translation="MISIKNVNKYYGKIQVLKDVSIEIESGEIFGIIGHSGAGKSTLL
                     RCINGLEEYQEGSVLVSDKEVKSLNEKQMRDLRKELGMIFQHFSLLERKTVFDNVALP
                     LECFGYSKAEIKKRVLELLEVVGISEKKNDKPRNLSGGQKQRVAIARALALNPQVLLC
                     DEATSALDPNTTKSILSLLEDINKKLGITIIVVTHQMEVIKQICGRVAIMENGEVLEV
                     GDTEEIFLRNTKGLRKLIGEESIILPKGTNIKILFPKDISNEAIITTMARELNIDVSI
                     IFGKLEQFKDDILGSLIINISDKSGEQVKQYLTSKGIRWEEMINE"
     regulatory      complement(6175..6281)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(6571..7515)
                     /locus_tag="ACGI59_003361"
     CDS             complement(6571..7515)
                     /locus_tag="ACGI59_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFG9741129.1"
                     /translation="MSRAERLIELMITINAKRSFTAGELAEEFSVSKRTILRDLQVLE
                     SIGFPLYSKVGAAGGYHVLKERILPPIAFSESEVKSIFFAYQSLEYYNDLPFEQETIS
                     VLKKFLNCIPNDIQYNIENIRRKFVFWTPDRHCSTPLLKELFNIVMNEFTIKIEYSSK
                     QKNSVRTIVPIGLYAMNGLWYCPAYCIKSESIKEFRVDRIVKILSIENLSSKKYKVLS
                     SIHDYLKNMEVGTDYHIKINLTDEGVKRCETEFLLARGLKILSKGGYIDMYIPKSTLN
                     WVAEYFLTFGKNATIIEPIELKHLIKSKVLELYNHHCI"
     gene            7626..8093
                     /locus_tag="ACGI59_003362"
     CDS             7626..8093
                     /locus_tag="ACGI59_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6194 family protein"
                     /protein_id="HFG9741130.1"
                     /translation="MENDTIKADDILNYCLSNLDDVVLMDSWGERAIYYNPNGVLKRG
                     VYVLTIKEKDSNNDKGSLVSRPNVYRVNIGLKKETFIEMFGYIPKRPGVGQIVDMDFD
                     FTKLDTIMPHPIYSWMGWICVLSPTEKTFENFKTLIGESYNFAKQKFKKRKNK"
     gene            complement(8263..8862)
                     /locus_tag="ACGI59_003363"
     CDS             complement(8263..8862)
                     /locus_tag="ACGI59_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9741131.1"
                     /translation="MKLKRTLFVGLALVLAIGITACTKNNEKPSENTGKNPNESVNNN
                     EGYMNYYSTSYNDYIAGLNRYSIYDTPESINNQFKDKEYPGNEKYLSDVKAAYKDSRD
                     KIQSFVTSLKKDGKTEDTELKKMNDDLIAEGERLVKDIDKRIEKLDKVSDEDMKKGQN
                     DFIKLVHGTEDVGNDIGSGFRKMIKDMNDRLGITGNNKK"
BASE COUNT         2889 a         1482 c          874 g         3708 t
ORIGIN      
        1 tacatatctt acctatataa aaacttattc tatataaaaa cgccataaaa aatcttttgc
       61 ttcttctgca taatctatat ttatacgttt actagttttt atatctaact catctttact
      121 aaatactttt tttcctttat catcataata aaaatcactt atatataatt cttcacccat
      181 tatactctta ctatttagac ttctatctat ttttaaagct ttgcaaagtt ttcctggtcc
      241 gttagtaata ttttttactt gatatttact caactcagtg taagctttgt tgtatctatt
      301 catagatatc tcatctaaag aagttatagg ctctacccct cttattaata cacactctgg
      361 cacattttct atatttgcag ataaattcaa gcaattatac ataccataaa ttaaataaac
      421 atatatatgt ccaccatcta aatataatgg cttagttcta tctgtctttt tattgccata
      481 tacatgtgct cctttatcat ttacacccat gtatgcttct gtctctatga ttttggtaac
      541 aattacttta ttttcatatt tccttattag atatttacct aatattgatt tggctaaatc
      601 aattccattt tttctaaaaa aatccttttc caaaatttca cctcataaaa tttatttaaa
      661 acgcatcaaa tacaactaaa gtaataaata aaattaactc ttattaatgt gatatatata
      721 aacaataaat ttagacattt attatttata tatatcaaaa aattctatct caaacttttc
      781 aatattatca ttgtaatttg aattatcata tacttcttca acaacctctt taattgggaa
      841 tataactttt acattagttc cttctcctac cttactatct atgtaaatct tcccattttg
      901 aatttcaatc aatgattttg taaggcataa acctatacca cttccaaatt catttgatat
      961 accactgttt tcaatttgtt taaatcgatt aaatatatcc tttaattttt ctttgtctat
     1021 gcccactcca gtatctttta taattatctc tactgtttcg tcttttttat atatacctac
     1081 aaggatttct cctccttcta aagtaaattt tatagcattt gataatatat ttagtattat
     1141 tctttctatc gattctgggt caagccctac cataacagtt tcttcatctg tatcaaaaat
     1201 taaatctatt cctttagact tagcataact actaatagat gtaactattc tttctgttat
     1261 gtcaataata tcaaaattaa ctgctttaat cttatatatg tctgacttaa cttgagaaat
     1321 atttattata ttatctatta atctcaataa tctaaacata ttttgtttag ttatttttat
     1381 atgttctttt agtatatcct gaaaatcaat tatttttcct tttttataaa ggttttcaat
     1441 aagttgaact gaactatata tattatttaa aggtgtctta agttcatgag atatattagc
     1501 ataaaactcc atcttcattt ggtcatactg tctttgtttt attaatgctt ttttaagtct
     1561 ttcagactct ctttgcacac ttaaatcttt aataattctc actctacttc ttttctgtcc
     1621 aaagtataat gttcttgata tcatttttac ttctttaatg tgattatttt catttactat
     1681 atggatactc ttctctcttt ctcctatatt taaattgttt atgtattttt ttactaatat
     1741 atttctatat ttttgtgttg taactcctaa tacatcacta gaaattccga acaactctct
     1801 aaaggctcta tttgtatata gtactcttga tgtacttaaa tcctcaagaa aaatagcctc
     1861 tggtataaca tccagtaatt tttcatatat tattttttgt tcatttactg ttctaagaat
     1921 atttatctct ttagttatat ctctaaatgt aagcaattta aagagtttat tatctgacca
     1981 atagtctcca actctaactt cttcaacatg ttcttcacct tctgagtcag acatcacaaa
     2041 attaactata cagtttgctg tgtcattgta acttactatt tcagctttct tttcttgtgt
     2101 tagatttaat cttttaaatt ccctattgtc aaagaaatct atgttaaaag tttcaaaata
     2161 ttgttctaat gtcatatcga ctaaatcact tgaaacacat ttaaaactct tttcatattt
     2221 tttatttata cttattacct tattcttctt tatgccacct tcagcacttc ttacaagtat
     2281 aggagatgga atatttagta taatgttttc atagtcttta tttatatttc ttatttcttc
     2341 atctattttg atttcatttg taatatcatt taaacttatt aagtaattgc tttcgtcttt
     2401 attattactt aaacttaaca aataaatttt atcatttaac tctaccagtt tatcaattat
     2461 acttatatcg ttatcaaatt tcttcaattt ttcagcaact ttttcatctt cctttataaa
     2521 agttaatatt ttataataat tattcatatc ctttgaaaat tcatctttaa atttattatt
     2581 tgtgtaactt atattaaaat catagtctgt tactatccat acatttggta ttgtatccag
     2641 agcttgtcta agtaattctt ttctctggtc tatgtagttt tttaatgttc tttgtataat
     2701 catatccttt tctattgcta aatttatctt tcctaattgc tctgactttt tacttaattt
     2761 tttattgatt gaatcaagtt cataatatgg atacactact ctattattaa gactgtatac
     2821 atatacataa taagtatggt tacataatag taaatatatt actcctatta atctatctga
     2881 atctactata ttaatcttag aaattgctac aaaacatata atagttgtaa aaaaaacaaa
     2941 atatgttaaa tcttttatta acttatagtg ctcaatgtta ctatcaagtt tattcaacaa
     3001 atatagtaaa ttaaacatag atatacaaat attaattaat ataataatca tattccagtt
     3061 aatatcacta aaaatatttc ttataaagta atctaatatc acaaaagcac tataaaaaat
     3121 tagtattggg cttattctta gtttttttac tttagaaatg gttaagtata taaaagtaga
     3181 catattaatt atgcttaagt taagcaatac aatttggaat ttttgaattt ttaaatttac
     3241 tactcctaaa atacttacaa aaactaatac aagacaccct actttcaata tatttataaa
     3301 ttcatctgtt ttaaagctac ttatataaac tatagaatat ccaaatatcc aaagtaaaat
     3361 tattctatac atatttatag aaactgtata caaatttata tttaaaagtg caataaccaa
     3421 aaataaccca ttagctagta tatagacctt attcttactt aaagttcctt tagattccat
     3481 attccccacc acttcttcat ttttatatag ttttacctat aaatatgtta ccacaattct
     3541 tccaaaaaat ttgtcacttt ctagttataa aaaaaaatga gtaaatattt ttaattttac
     3601 tcattttgta gattttgtat attcagttta aaaagctgga actatacttc ctttatattc
     3661 atcttgtata aattttttaa cttctttact attcatagcc tctgataaag ccttaatctt
     3721 atcactatct ttattatttt ctctacaagc tattatattt acatatggag aatcacttga
     3781 ttctattact attgcatcct tagtaggatt taagttagct gttaaagcat aattagaatt
     3841 tattactgct ccgtcaacat cttttaatac tgttggtaat tgctctgcat tcatttcttt
     3901 tattactata tttttaggat ttttagttat atctttttta gttataagct ctccatcttt
     3961 tacttctatc aatttatttt ttgctaataa tttaagtgct ctagctccat tagttgcatc
     4021 atttggcaca gctataacag ctccatcttt taattcatct aatgacttaa ctttttctga
     4081 atagaatccc attggctcta tatgaacttt agatgtataa gtaagtttat atcctttctc
     4141 cttcacagtt tcctctaaaa atggaatatg ttgaaagaag ttagcatcca aagacccttc
     4201 atctaaagct gtatttggaa gtacataatc atcaaatatt tttacttcta aatcatatcc
     4261 tttttctttt aacaaaggtt ttgcaacttc caatatcttt gcatgtggat ttgatgaagc
     4321 tcctactagt atttttttat cctctttgtt tgaacaacct actgctgata ttgctattgc
     4381 tgacaccaat gctactgata ataatttctt taatttcatt tttactcccc ctctttaaaa
     4441 tgtttataat taatttattt tagtttctta taagccagat ttcctactcc ctggattatc
     4501 tgaactaata ttataagtgc tattacagta tataccatta ctgctgtatc aaatctttga
     4561 tatccataaa taagtgctat attacctaat cctccagctc ctactgcacc tgccatagct
     4621 gtataaccaa gtatagatat tattgataat gtaattccag atactattga aggcatagct
     4681 tcctttatca taactttaaa tactatttgt ctttttgtag ctccaaaaga ttttgctgct
     4741 tctatcaatc ctttatcaac ttcatttaat gagctttcta taattctagc gataaacggt
     4801 gctgccccaa tagttattgg cactattgca gcagtttccc ctatacttgt tccaacgatt
     4861 aatctagtta ttggaatcat tgcaactata agtatcataa atgggaaact tctaaatata
     4921 tttactataa atcctaatgc actatttatt gtgctatttg gttttaatcc atctggtttt
     4981 gtaactacta atatgattgc taatataaat cctaatattg ttgccactat agttggtact
     5041 ataaccatat acaaagtttg taacaatgca tttggaaata gtgttgttaa aaagtcaatt
     5101 aaactattca tttatcattt cctcccatct tatgccctta ctagttagat attgttttac
     5161 ttgctctcca cttttatctg aaatgtttat aataagtgaa cctaaaatat catctttaaa
     5221 ttgttctaat ttcccaaata ttatagacac atctatattt agttctcttg ccatagtcgt
     5281 aataatagct tcattagaaa tatctttagg gaatagtatc tttatatttg ttccttttgg
     5341 caatattata ctttcttcac ctattaattt tctaagtcct ttagtatttc ttaagaatat
     5401 ttcttctgta tctcctacct ctaatacttc tccattttcc attattgcta ctcttccaca
     5461 aatctgcttt ataacttcca tttgatgagt tacaactatt attgttattc ccaatttttt
     5521 atttatatct tcaagcaatg aaagaattga ctttgtagta tttgggtcta atgctgaagt
     5581 cgcttcatca caaagaagta cttgtggatt tagtgctaaa gctctagcaa ttgcaaccct
     5641 ttgtttttgt ccgccactta gatttctggg tttatcattt ttcttttcag atattccaac
     5701 tacttctaat aactctaaaa ctcttttttt aatttctgcc tttgagtatc caaaacattc
     5761 taatggcaat gctacattat caaatacagt ttttctttca agcaatgaaa agtgttgaaa
     5821 aatcattcct aactcttttc ttaaatctct catttgcttt tcattcaatg actttacttc
     5881 tttatcagaa acaagtacac tgccttcttg gtattcttct aatccgttta tacatctcag
     5941 tagtgttgat ttccctgcac cactatgacc tattattcca aaaatttctc cagattctat
     6001 ttcaatactt acatctttaa gcacttgaat ctttccataa tacttattaa cattttttat
     6061 gctaatcata aaatttcctc ccatatattc tgctcagttt ttatgtttat cctctcttat
     6121 gtattacaac aaaataaaag cccgagtata aactcaggct tagaaagcta tttatactca
     6181 tctatcaact atattagttg caggatttag caccaatgta tataaaatat actggttgcc
     6241 gggtttcaaa gggccagtcc ctccaccact cttgataaga cgttatttaa tttaattcta
     6301 ttatcatttg ttgctatgtt atatatgata tattcattaa tagtatttgt caataaattt
     6361 atttaaattt tttatttttt attatcgtta attaactatt tttaattagt tttctagctc
     6421 ctaacacttt atattttaat gtctatataa ctatagaaca tcattataag aaataaatta
     6481 aataacatca atctatctaa atcatctgta aagtaaatta agaaaattaa cattttatcc
     6541 aaactctatc ctactattaa ataaaataat ttaaatacaa tgatgattat atagctctag
     6601 tactttagac tttatcaaat gtttaagttc tataggttct attattgtag catttttccc
     6661 aaaagttaaa aaatattcag ccacccagtt aagtgtagat tttggtatat acatatctat
     6721 gtatccacct ttagacaaga ttttcaaccc tcttgccaac aaaaattctg tttcacaacg
     6781 ttttactccc tcatctgtca aattaatttt aatatgataa tctgtaccta cttccatatt
     6841 cttaagataa tcatgtatag aagataaaac cttatatttc ttacttgata agttttctat
     6901 actcaaaatc ttcacaattc tgtctacacg aaattcctta atagattctg attttataca
     6961 gtaagctgga caataccaaa gtccattcat ggcatacaaa ccaataggaa caatggtacg
     7021 tacactattt ttttgctttg atgaatattc aattttaatt gtaaattcat tcattactat
     7081 attaaacaat tctttcaata atggtgttga acagtgccta tctggtgtcc aaaaaacaaa
     7141 tttacgcctt atattctcaa tattatattg aatatcattt ggtatacaat ttaaaaattt
     7201 ttttaaaaca gaaatagttt cttgctcaaa tggcaaatca ttataatatt ctagtgactg
     7261 atatgcaaag aatatagatt ttacctcact ttctgaaaat gcaatcggtg gaagtatacg
     7321 ctctttaagt acatgatatc caccagccgc tccaactttt gaatataatg gaaatccaat
     7381 actctccagc acctgtaaat ctcttagtat agtacgtttt gatacagaaa attcttctgc
     7441 taattctcct gctgtaaaac ttctttttgc atttattgtt atcatcagtt caattaaacg
     7501 ctcagctctt gacatattta tactcctaat ttaaatatag tgacaataat tgtcactatt
     7561 atatgataag atattcttgc atctaaagca ataataatag taaagaaaga agggattgat
     7621 actttatgga aaatgatact attaaggctg atgatattct caattattgt ctatcaaact
     7681 tagatgatgt tgtactaatg gatagttggg gggaacgagc aatttactac aatcctaatg
     7741 gtgttttaaa gcgaggggta tatgttctta ccattaagga aaaagacagt aataatgata
     7801 aaggttcgtt agttagtcgt ccaaatgtat accgtgtgaa tataggatta aaaaaagaaa
     7861 cttttattga aatgtttgga tatattccaa agcgtccagg tgtaggtcaa atagttgata
     7921 tggattttga ttttacaaaa ttggacacaa tcatgccaca tcctatctac tcatggatgg
     7981 gatggatatg tgtcctaagc cctactgaaa agacatttga gaactttaaa actttaatag
     8041 gagaatctta caattttgca aaacaaaaat ttaaaaagag aaaaaacaag taaaaacgtg
     8101 tgaaaataac ctctaaataa attataataa aaatattaag gttacaagat aaaaattaat
     8161 atcttaataa catgcaaaaa agcgaagcca aaaaatgtat tatttataag gcttcgctat
     8221 atctcaaata atttatttca aatttcaaaa ctatttattc tattatttct tattatttcc
     8281 tgttattcct aatctatcat tcatatcttt tatcattttt ctaaaaccac tacctatatc
     8341 atttccaaca tcttcagttc catgaactaa ttttataaag tcattttgac cttttttcat
     8401 atcttcatca gataccttat ctaatttttc tattctctta tctatatctt ttactaatct
     8461 ttccccttca gcgattaggt catcattcat tttctttaac tctgtatctt ctgttttacc
     8521 atctttttta agagatgtta caaatgattg tattttatct ctactatctt tatatgcagc
     8581 ctttacatca cttagatatt tttcatttcc tggatattct ttatctttaa actgattatt
     8641 tatactttct ggagtatcat aaatagaata tctatttaga cctgctatat aatcattata
     8701 gcttgttgaa taataattca tatatccttc attattattt acagattcat taggattttt
     8761 tcctgtattt tcacttggtt tttcattatt tttagtacat gcagttatac ctatagctaa
     8821 tactagtgct aatcctacaa ataaagttct ttttaatttc atttttatta ccctcctaga
     8881 tgttttatac tataaaaaat tttaataatt ttctttctta atttagtatt tctatatttt
     8941 tatattttgt tta
//
LOCUS       DAYAPA010000149         8833 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.153, whole genome shotgun sequence.
ACCESSION   DAYAPA010000149 DAYAPA010000000
VERSION     DAYAPA010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8833
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.153"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            92..433
                     /locus_tag="ACGI59_003364"
     CDS             92..433
                     /locus_tag="ACGI59_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HFG9741132.1"
                     /translation="MKEENFINVEYEDKRFSINSKEVLYIEKQEYEVKIYTESGNYEV
                     GENAPCIRKLLKEENFFKCSNSYIVNLDKCEFYLSNNCIFVNNNFIRILGSERKKKLK
                     KKLEKLYDYED"
     gene            584..997
                     /locus_tag="ACGI59_003365"
     CDS             584..997
                     /locus_tag="ACGI59_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1653 domain-containing protein"
                     /protein_id="HFG9741133.1"
                     /translation="MLELKNNLGISERKLKYPCIYKHFKGKYYATMGLSKAIDDIENI
                     CEIYGKENLIQNRNKYKLVIRHTEREEDIYVYRDLDGNFYHKKEEDTNDLVLYKTLYD
                     DTGIFARPLDMFLEKVDTDKYVNSIQEYRFEEVYK"
     gene            1199..1840
                     /locus_tag="ACGI59_003366"
     CDS             1199..1840
                     /locus_tag="ACGI59_003366"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFG9741134.1"
                     /translation="MGNTFYIVRHGQTDWNILGKTQGHGNSDLTPQGIEQAKELSEDI
                     GKYSIDYIFSSDLGRAMQTAQILGDKLNIEVQKTEALREMGFGVWEGLLIKEIQKDYS
                     DIYATWRNEPHLVNIPEGETLKIIKERVDAFIKELNEKYDNKNIILVTHSITLRVMLL
                     SFLESGMENIYRIKQDNTALNIVEFKDYGPVIVKMNDTSHIKNHVKINNSALE"
     gene            1864..3117
                     /locus_tag="ACGI59_003367"
     CDS             1864..3117
                     /locus_tag="ACGI59_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFG9741135.1"
                     /translation="MSKVIVVGGGASGMMAALSASKNNNEVILVERNGELGRKLRATG
                     GGRCNFTNNREIEDFFDKVVSNKKFLYSSFYTFTNKDLISYFESRNLEYKIEEENDHK
                     VYTKNDKSIEVIEVLNKDLLNHNVKIMYNKKVIDIITEEIALKDDSNKDKSKYLIKGI
                     ILDNGDKILGDKVIISTGGVSYSKTGSDGSMYKILKKHGHTLNKLYPALVPLTIEEKW
                     IKDLQGISMKNVEISCKIKKRKISKSGDMLFAHFGITGPCVLIMSSYINKIIEKEKVE
                     LNIDFLPNLSTDEISSIIRAFPNKNVLNNLKQILPQNFLKEIFSLLSLVDKKASDLSK
                     ADEIRIIEYIKNMKLTCNGTTGINTGMVTSGGISVKEINSSTMESKLVKNLFFTGEVI
                     DIDAETGGYNLQIAFSTGYLAGISV"
     gene            3163..3813
                     /gene="cmk"
                     /locus_tag="ACGI59_003368"
     CDS             3163..3813
                     /gene="cmk"
                     /locus_tag="ACGI59_003368"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFG9741136.1"
                     /translation="MGNLVIAVDGPAGAGKSTIAKIVAKKLNINYIDTGAMYRAVTYK
                     CLKSGIDVNNEKEVIQIAENSDIDFKDNNIYLDKEVINEEIRTIEVSNNVSNVAKIKE
                     VRQLMVEVQRKIGMKNSVILDGRDIGSYVFPDADYKFFLVATPEERGNRRYKELCNKG
                     YNTTLEEVIEDIIRRDEIDSNREFAPLVKANDALEIDTTGKTIEEVVEEVVSKINL"
     gene            3864..4460
                     /locus_tag="ACGI59_003369"
     CDS             3864..4460
                     /locus_tag="ACGI59_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFG9741137.1"
                     /translation="MNFYRFVINIFKGFSKIFFKYEVIGAENIPDRGNIVIASNHKSN
                     LDPIFLAAAIENREIAAIAKKELFKVKPLGFILKKLHVMPINREKPDVSTIKTILRSV
                     RDGYVLGIFPEGTRIKGDSFGKAKAGLSVFTIKSKSKVVPVSIISKYKLFSKVIVYIG
                     EPISFEEHFKEKLSNDDHERISQEILEVIKQNYFKYSK"
     gene            4473..5312
                     /locus_tag="ACGI59_003370"
     CDS             4473..5312
                     /locus_tag="ACGI59_003370"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HFG9741138.1"
                     /translation="MNIKIAKNAGFCFGVKRAMKMAWDEVEKNDSGIYALGPLIHNKQ
                     AVAKYEEKGLKTVNEIDTIPNHENMIIRSHGVPENIYKEAKDKKLKIVDTTCPFVKKI
                     HTVVSEYHNKGYEIIVIGDMKHPEVIGINGWCENSAIIIKTLEQMENMEFDNSKRYCL
                     VAQTTINPELYISIVNKLSDKLEEIVFNDTICSATKTRQESAKELAKEVDCMIVIGGK
                     HSSNTQKLVKVCEDLVPTFAIETKDELDVNTLKKYKNLGITAGASTPNWIIEEVVTFL
                     ENL"
     gene            5557..6711
                     /locus_tag="ACGI59_003371"
     CDS             5557..6711
                     /locus_tag="ACGI59_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese transporter"
                     /protein_id="HFG9741139.1"
                     /translation="MEMLISASEEAFLHVGSMIGFFILLFGYINYKTSGNFTNIISKN
                     RKFQPLIGALIGAIPGCGGSLAIMPLYINGKLSFGAIIASLIASMGDAAFVLISSNIK
                     MYFFVTIVSTITGIITGQLVDYFKLEEKLGLKNRRKSDKYDISKNNPKENKEQHNHDE
                     IILDTLAKSHGNTNRLAFIITHGKGYKIYIGIIVIGFIFMSLAHSGLNLPIIEKLHSL
                     EEVIAVIGILFSIIYMWCFKKVFKNSNQKEEENKKISLREMLIHSVGEISFVITWIFI
                     AYLIYDLIILILGGDEYLVKLVLSTGVISVFIGAGLGLIPGCGIQIVLMSFYLKGTIP
                     LGAVIANSISQDGDALFPLLAMDKKSSLWAMIITTIPAILVGMIVYAFLG"
     gene            7017..8714
                     /gene="ade"
                     /locus_tag="ACGI59_003372"
     CDS             7017..8714
                     /gene="ade"
                     /locus_tag="ACGI59_003372"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFG9741140.1"
                     /translation="MGNLKRQCDVSSGREKADIVLKNGTIINVFTEELITGDVAIVGD
                     TIVGIGDYKGNVEIDCSNKYISPGFIDAHMHIESTMVMPIELSKKLLKSGTTTIIADP
                     HELVNVKGVSAIDFLLESTKDIPLNVYIMVPSSVPATSFETNGVGKFSAKDMESYVNN
                     PRILGLGEVMCFNDVINSENEILDKLELFKNKVVDGHAPNINGKSLQTYVCAGIENDH
                     ECITFDEVYEKLRAGLKILIREGSAAKNLKSIVSGMLKHNLPIEEFMFCTDDKHLDDI
                     EKQGHIRWNIKCAIDLGMEPVRAIKVATYNSAKAYGLRKIGAIGAGYKADIVVLNDLD
                     KMEVDSVYKDGNLVNEEMFSNYNYEIKDKELLNTVKFKYINKEKIQLKVSEKNYVMEI
                     VPYQILTNKVYESLPCADGYFVPNKEYSKLCVVERHRMTGNVAIAPLKGFGIKNGAIA
                     TTVAHDSHNIIVAGDNDDDILVAINYLKEIQGGYVIVSNGKVLAHLSLQVAGLISTFT
                     AEEVQEITDNMLEIARKMEVPEYVDPFITLSFMALPVVPQIRLTDLGLFDVEEFKFI"
BASE COUNT         3621 a          847 c         1574 g         2791 t
ORIGIN      
        1 ttaagtaaat cagtcttaca tatatttccg atagattata ttgataaaaa acactaaaat
       61 taaattaata aaagtacttg gagggatgga aatgaaagag gaaaatttta ttaatgttga
      121 atacgaggat aaacgttttt caattaatag taaagaagtt ttgtacatag aaaagcaaga
      181 gtatgaagta aagatttata ctgaaagtgg taattatgaa gttggagaaa atgctccttg
      241 tataagaaaa ctattgaaag aagaaaattt tttcaaatgc agtaatagtt atattgtaaa
      301 tctagacaag tgtgaatttt atctcagcaa taattgtatt tttgtgaata ataattttat
      361 aagaatttta ggttcagaaa gaaaaaagaa attaaaaaag aaattagaaa aattatatga
      421 ttatgaagat taatattggg aggctgtatt ttatacagcc tctttattta ttgattgtat
      481 tattaataaa ttttaaatta tgataaaata taaaacaagt aacttatttc agaataaaat
      541 actttttaaa agaaagggac gagatatggg agattataat aaaatgcttg aattaaagaa
      601 taacttaggt atcagtgaaa gaaagttaaa atatccttgt atctataaac atttcaaagg
      661 gaaatattat gctacaatgg gtcttagcaa ggctattgat gatatagaaa acatatgtga
      721 aatatatggt aaagaaaatt taatccaaaa cagaaataaa tataaattag ttattagaca
      781 tactgaaaga gaagaagata tctatgttta tagggattta gatggaaact tttatcataa
      841 aaaagaagaa gatactaatg atttagtttt atacaagacc ttatatgatg atacaggaat
      901 atttgcaaga cctttggata tgtttttaga aaaagtagat acagataagt atgtcaattc
      961 aattcaagaa tacagatttg aagaagtata taaataatat aagagaaata tgtttataaa
     1021 tttagaaaaa ttctttaaca gtacaataat ttgcagaaat acaaatgaga tttattgtaa
     1081 ataatatgtt agattagatt ataaaataga aacaaagtaa agtattatat gatataatta
     1141 agttttgaga agtgataatt tatatttaat aaaaaatagg catatattag gaggacatat
     1201 gggaaatact ttttatatag taagacatgg acaaacggat tggaatatat tgggaaaaac
     1261 acaaggtcat ggtaattctg accttacgcc tcaaggtata gaacaggcaa aagaactttc
     1321 tgaagatatt ggcaagtatt caattgatta tatttttagt agcgaccttg ggagagcaat
     1381 gcaaacagct caaattttag gagataagct caatattgaa gttcaaaaaa ctgaggcact
     1441 tagagaaatg ggattcggag tttgggaagg ccttttaata aaagaaattc aaaaagatta
     1501 ttcagatata tatgcaacat ggagaaatga accacactta gtaaatatac cagaaggcga
     1561 aactcttaag attataaagg aaagagtaga tgcttttata aaggaactaa atgaaaaata
     1621 tgataataaa aatataattc ttgttacaca ttcaattaca ttgagggtta tgttgttgtc
     1681 atttttagaa tcaggtatgg aaaatatata tagaattaag caagataata ctgctttaaa
     1741 tatagtagaa tttaaagatt atgggcctgt tatagtaaaa atgaatgata caagtcatat
     1801 aaaaaatcat gtaaagataa ataattctgc actggagtag gaatcaatta taaggagaaa
     1861 tttatgagta aagttatagt agttggtgga ggagcttctg gaatgatggc agctttgtca
     1921 gcttcaaaaa acaataatga agtaatatta gttgagagaa atggagaatt aggaagaaaa
     1981 cttagggcaa ctggtggcgg tagatgcaat tttaccaata atagagagat agaagatttt
     2041 tttgataaag ttgtcagtaa taaaaaattt ctctatagta gtttttatac atttacaaat
     2101 aaagacctta tttcgtattt tgaaagtaga aatttggaat ataagattga agaagaaaat
     2161 gaccacaaag tatacactaa aaatgataaa tctatagaag taatagaagt attaaataaa
     2221 gatttactca atcacaatgt aaagataatg tataataaga aagttataga tattataact
     2281 gaagaaattg ctctaaaaga tgattctaat aaggacaagt caaagtatct aataaaaggg
     2341 attattttag ataatggaga taaaatacta ggagacaagg ttattatatc aactggagga
     2401 gtgagttatt caaagacggg ttcagatggt tcaatgtata agattcttaa aaagcatgga
     2461 catacattaa acaaactata tcctgcttta gttccactta caatagagga aaaatggatt
     2521 aaagatttac aaggaatatc catgaagaat gtggaaatat cttgtaagat taagaaaagg
     2581 aaaatatcaa aatctggaga catgctattt gcccattttg ggataacagg accttgtgtt
     2641 cttataatgt cttcttatat taacaagatt attgaaaagg aaaaggtaga attaaatata
     2701 gatttcttac caaatttaag tactgatgaa atctctagta taatcagagc atttccaaat
     2761 aaaaatgttc taaataatct taagcaaata ctacctcaaa atttcttaaa agagattttt
     2821 tctctattat ctttagttga taaaaaggca agtgatttgt ctaaggcaga tgaaattaga
     2881 attattgagt atataaaaaa tatgaagcta acttgtaatg ggactacagg tataaacact
     2941 ggtatggtta cttctggtgg tatttcagtt aaagaaatta attcttctac tatggaatct
     3001 aaattagtta agaacttatt ttttacaggt gaagtaattg atatagatgc agaaactggt
     3061 ggatataatt tacaaatagc attttcaact ggatatcttg cgggtataag tgtataaata
     3121 taatttttta ataattaaat atgaataaat agaggtgtat atatgggtaa tttagttata
     3181 gctgtagatg gtccagcagg agctggaaaa agtacaatag ccaagatagt agcaaaaaag
     3241 ctaaatataa attatataga tacaggtgct atgtacagag cagttactta taagtgttta
     3301 aaaagtggta ttgatgtcaa taatgaaaaa gaagttattc aaatagctga aaattcggat
     3361 atagatttta aagataacaa tatatatttg gacaaagaag ttataaatga agaaataaga
     3421 acaatagaag taagcaataa tgtttctaat gtagcaaaaa taaaagaagt aagacagttg
     3481 atggttgaag ttcaacgaaa aataggaatg aaaaattcag taatactaga tggtagagat
     3541 ataggttcat atgttttccc agatgcagat tacaaattct ttttagtagc tactccagaa
     3601 gaaagaggaa atagacgcta taaagaactt tgtaataaag gatataatac tactttagaa
     3661 gaagtaattg aggatataat aagacgagat gaaattgatt caaatagaga atttgcacca
     3721 ttggtaaaag ctaatgatgc actagaaata gatactacag gaaaaactat agaagaagtt
     3781 gtagaagaag ttgtatctaa aataaatctt tagtttttta tgcatcataa tgaaaataaa
     3841 tgtagtgttg aagggagata aacgtgaatt tttatagatt tgtaataaat atttttaagg
     3901 gtttttctaa aatatttttt aaatatgaag ttataggtgc agagaatatt ccagatagag
     3961 gaaatatagt aatagcatca aatcataaat cgaatcttga ccctatattc ttggctgctg
     4021 ccatagaaaa tagagaaata gctgcaattg caaagaaaga attgtttaaa gtaaaaccat
     4081 tgggatttat attaaaaaaa ttgcatgtta tgcctataaa tagagaaaaa ccagatgtat
     4141 ctacaataaa aactatttta aggtcagtta gagacggtta tgtattagga atatttccag
     4201 agggtactag aataaaagga gattcttttg gtaaggctaa ggcaggtctt tctgtattta
     4261 caataaaaag taaatctaaa gttgttccag tatcaataat atctaaatat aaacttttta
     4321 gtaaagtaat tgtatatatt ggagaaccaa tatcatttga agaacatttt aaggaaaaat
     4381 tgagtaatga tgaccatgaa aggatatcac aagagatttt agaagtgata aaacaaaatt
     4441 actttaaata ttcaaaataa gggggtaaag ttatgaacat aaaaatagca aaaaatgctg
     4501 gcttttgttt tggagtaaaa agagcaatga aaatggcatg ggatgaagtt gaaaaaaatg
     4561 attctggcat atatgcttta ggtcctctta ttcataacaa gcaagcagta gcaaaatatg
     4621 aagaaaaagg tcttaagact gtgaatgaaa ttgatacaat accaaatcat gaaaatatga
     4681 taataaggtc tcatggagtt cctgaaaata tatataagga agccaaagat aaaaaattaa
     4741 aaattgtgga tactacatgt ccatttgtaa agaaaataca tacagtagta agtgaatatc
     4801 ataataaagg ctatgaaata atagttattg gagatatgaa gcatccagaa gttataggta
     4861 taaatggatg gtgtgaaaat tcagctataa taataaagac cttagaacaa atggaaaata
     4921 tggaatttga taactccaaa agatattgtc ttgttgcaca gactacaata aatccagagt
     4981 tgtatataag tatagttaat aaactatcag ataaattaga agaaatagta tttaatgata
     5041 caatttgttc agctacaaaa acaagacaag aatcagctaa agagttagct aaagaagttg
     5101 attgtatgat agttatagga ggtaaacaca gttcaaatac tcaaaagtta gtaaaggttt
     5161 gtgaagactt agtaccaact tttgcaatag agactaaaga tgaattggat gtaaatacgc
     5221 ttaaaaaata taaaaatttg ggtattacag caggggcatc tacacctaat tggataatag
     5281 aagaagttgt aacattcctt gaaaatttat aatttgatag gtaataaaaa taggtggaca
     5341 ttgttataat ttatcataat atccaccttt gatttcatat tacatgtggt aaatagataa
     5401 cctaatatca aaaagtgtgt atcttaagat gaaaaactaa gagtataaga tgcgcacttt
     5461 ttaatattag aaattcaaaa gaaatattga tattgattat caatagaaaa tagaatataa
     5521 tataattgtt gcaaaattag tctaggaggt aaattaatgg aaatgttgat atccgcttct
     5581 gaagaggcgt ttttacatgt aggttctatg attggatttt ttattttgtt atttggatat
     5641 attaattata aaactagtgg aaattttact aatatcatat caaaaaacag aaaatttcaa
     5701 cccttaatag gtgctttaat tggagctata ccaggttgtg gcggaagttt ggcaataatg
     5761 ccattgtata taaatggaaa attaagcttt ggagcaataa ttgcaagttt aatagctagt
     5821 atgggagatg cagcatttgt acttatttca tcaaatatta aaatgtattt ttttgtcact
     5881 atagttagta caattacagg aataataact ggacaattag tggattactt taaactagaa
     5941 gaaaaattag ggttaaaaaa ccgtagaaaa tcagataaat atgatataag taaaaataac
     6001 cctaaagaaa ataaagaaca acataatcat gatgaaataa ttttagacac tctagctaaa
     6061 tctcatggaa atacaaatag acttgcattt ataataacac atggaaaggg atataaaatt
     6121 tatataggaa ttatagtaat cggttttata tttatgtcac ttgctcactc tggacttaat
     6181 cttcctataa ttgagaagtt acattcattg gaagaagtaa tagcagtgat tggaatatta
     6241 ttttcaataa tttatatgtg gtgttttaaa aaggtattta aaaattctaa tcaaaaagag
     6301 gaagaaaata aaaagatttc tttgcgagaa atgcttatcc attctgttgg agaaatatca
     6361 tttgtaatta catggatttt tattgcatat cttatttacg atttgataat attaatttta
     6421 ggtggagatg aatacttagt taaattagta ttatcaacag gtgtaataag tgtatttata
     6481 ggtgcaggtc ttggacttat tccaggttgt gggatacaaa ttgtgcttat gtcattttat
     6541 ttgaagggaa ctataccact tggtgcagtg atagccaact ctatttctca agatggagat
     6601 gcacttttcc cacttttagc aatggataaa aaatcttctt tatgggctat gattattact
     6661 actataccag caatacttgt aggtatgata gtttatgcat ttttaggtta aatataatta
     6721 tgaataaaga agtattaaaa aaataataaa gcacataaat ttatttataa gaatagtgaa
     6781 gattaaatat aaaaataaaa aattcaatat gaattaaatt attgcttaaa tattaatgag
     6841 aacatgggag ataatattat tcctcatgtt ctctctattt aattaaagga attatggaac
     6901 aaatttcaag tgatactttc tatcaaaact catatataat atatattaat aaattaaact
     6961 ataatacgta atacagtcag tatattgata taaaaaactt aggaggagtt gtaaaaatgg
     7021 gtaatttaaa aagacagtgt gatgtttcaa gtggaagaga aaaagctgac attgtactaa
     7081 aaaatgggac aatcataaat gtatttacag aagagttaat aacaggagat gtagcaatag
     7141 taggagacac tattgttgga attggcgatt acaagggcaa tgtagaaatt gattgtagta
     7201 acaaatatat atcacctggc tttattgatg cacatatgca tattgaatct acaatggtaa
     7261 tgccaattga attatcgaaa aaattattaa aatcaggtac gacaactatc atagccgacc
     7321 cacatgagtt ggtcaatgta aaaggagtta gtgcaataga ttttttatta gaatcgacaa
     7381 aagatatacc acttaatgta tatattatgg tgccttcatc cgtacctgca acaagttttg
     7441 aaactaatgg agttggtaaa tttagtgcaa aagacatgga gagttatgta aataatccta
     7501 gaattttagg tttaggagag gtaatgtgtt ttaatgatgt tataaattct gaaaatgaaa
     7561 tcttggataa actagagctt tttaaaaata aagttgtaga tggacatgca cctaatataa
     7621 atggtaaatc acttcagaca tatgtatgtg caggaataga aaatgaccat gaatgtataa
     7681 cttttgatga agtatatgaa aaattgagag caggattaaa aattttaata agagaaggaa
     7741 gcgcagcaaa aaatttaaag tctatagtaa gtggtatgct aaagcataac ttgccaattg
     7801 aagaatttat gttttgcaca gatgataagc acttagatga tatagaaaaa caaggtcaca
     7861 taagatggaa tataaaatgt gcgattgatt taggtatgga acctgttagg gctataaaag
     7921 ttgcaactta taattctgca aaagcatatg ggttaaggaa aattggagca ataggtgcgg
     7981 gttataaggc agatattgtt gtattgaatg atttagacaa aatggaagtg gatagtgtat
     8041 ataaagatgg taatttggta aacgaagaga tgttttctaa ttacaattat gagattaaag
     8101 ataaagaatt gcttaataca gtcaagttta aatatattaa taaagaaaaa atacagttaa
     8161 aagtaagtga aaaaaattat gtaatggaga ttgtacctta tcaaattttg acaaataagg
     8221 tatatgaatc tttaccatgt gcagatggat attttgttcc aaataaagag tattcaaaac
     8281 tttgtgttgt tgagagacat agaatgactg gaaatgtagc aattgctcca ctaaaaggat
     8341 ttggcataaa aaatggagca attgctacta ctgtggccca tgattctcat aatattatag
     8401 ttgcagggga taatgatgat gatattctag tagctataaa ttacttaaag gaaatacaag
     8461 gtggttatgt aattgtatca aatggaaaag ttcttgcaca cctaagtctt caagtagctg
     8521 gattaattag tacttttaca gctgaagaag tacaagaaat tactgataat atgttggaga
     8581 tagcaagaaa aatggaagtt ccagagtatg tagacccttt tattacactt tcttttatgg
     8641 cacttccagt tgttccacaa ataagactta cagatttagg tctatttgat gttgaggaat
     8701 ttaaatttat atagatagta taaaagtaat aataaatgag gctttctcaa aatagagata
     8761 agtctctttt tattgttgaa atgatgaaaa tatatttgat ttcatacaaa aaagagcatc
     8821 tcatgaacta aaa
//
LOCUS       DAYAPA010000150         8593 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.155, whole genome shotgun sequence.
ACCESSION   DAYAPA010000150 DAYAPA010000000
VERSION     DAYAPA010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8593
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.155"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            227..1213
                     /locus_tag="ACGI59_003373"
     CDS             227..1213
                     /locus_tag="ACGI59_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFG9741141.1"
                     /translation="MKKITINDIANLAGVSKSTVSRYLNNKDISDSTKEKIKTIIDEY
                     GYEPNAFAQSLRAKKTYFIGIITPCLDSFVKSKIMMAIDEELKELKYTSLIINTNRKI
                     RSEIDSISKLASLKVDGIILIGTEITKEHKNEIEKLDIPIVVVGQKVDGINSIVNDDY
                     GAGYKMGQYIANKGYKNIVYLGVDESDISVGLNRKNGVLNGLKDKGYDAKVFYTDFDQ
                     ETSIQRSGEMLESENPDIIICATDNIAIATMKEINKRGKNIPQDISVAGFGGYDVLSI
                     ISPKLTTIKFENKNAGKVAANTIVNLIQERKEPLLKEIKFELIEGESTINKN"
     gene            1549..3030
                     /locus_tag="ACGI59_003374"
     CDS             1549..3030
                     /locus_tag="ACGI59_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HFG9741142.1"
                     /translation="MYKEPKYRTILESTKEELNKLRNISLNDKYKPLFHIHPQHGLLN
                     DPNGLAYYNGKYHVFYQWYPYDATHGMKHWAYVSSDDFVNWNREDVALIPIESYESHG
                     AYSGNAIEVDGKLHMYYTGNIKYSAEDRYAYQNLAIMNKDGKITKYENNPIVSEIPKG
                     YTGHVRDPKVFKRKDKYFMLLGAQTSDKKGAIIVYESKNSIDWNFKGELNVKNIDEDF
                     GYMWECPDYINIDEKDILIFSPQGVEPKGFDYQNIYNVVYAIGNMDLDNLTFEIDTMK
                     ELEKGFDFYAPQTFIKDSQIILFAWAGMGEVLYPTDKNKWAHCLTVPRKLNIKNNKLL
                     QMPVDELIKLRYDETSGQNTIKNNINIIENDENVYELNINIKNIDSNKFGLELFSSQD
                     EGVKLEFNKLGNIVTLDRSNFKKVFGVEYGTNRKEYINIDENTNIKVLADRSILEIFI
                     NEGEVVFTSRIFAKENSNQIRVYSDKIVEYEYTKFKLKQGIEL"
     gene            3056..4027
                     /locus_tag="ACGI59_003375"
     CDS             3056..4027
                     /locus_tag="ACGI59_003375"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HFG9741143.1"
                     /translation="MKKVISIGEALIDFIPNQNGGKLKNVSEFRRVAGGAPANVSAVV
                     AKLGGKSSFISKLGKDAFGDYIIDVLNEVNVNTDYVLRTSKANTGLAFVSLKEDGNRD
                     FSFYRNPSADMLLEADEVKKEWFNNCHILHFCSVDLIDSPMKLAHKKAIEYALESNSI
                     ISFDPNIRLPLWDSEQSCKKAISEFLPFAHIVKISDEELEFITGENDIEKSLHKLFVG
                     NVELVLYTKGKDGVDAYTKKVKGMCKGVKVNAIDTTGAGDSYIGSFLYTLLYKQITLN
                     DIKEMKQETLNEYLEFSNYYAARSTTIKGAISSYATKEEITEFIKNL"
     gene            complement(4283..4885)
                     /locus_tag="ACGI59_003376"
     CDS             complement(4283..4885)
                     /locus_tag="ACGI59_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HFG9741144.1"
                     /translation="MEVCILMGSPKKKGNTAAILKPFIEELENYNSNIEVIWLYDCKI
                     EPCIACKKCQDNLFDFGCYCKDDVQKIFKKILMCDLIVFATPIYSWYCTSPMKALLDR
                     LVYGMNKYYGNEKGPTLWAGKPVALITTCGYRPEKGADIWENGMKRYCKHSQLKYIGM
                     LVERDLGKPNFMNKEKEEHSRQFAKQIYYILDNSIEVDNC"
     gene            5166..6377
                     /locus_tag="ACGI59_003377"
     CDS             5166..6377
                     /locus_tag="ACGI59_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFG9741145.1"
                     /translation="MYSNEDYKFIEENAPCYIYDETNIIEKCKELKNNILGFDFLYSI
                     KTNPYNKIVKSIAEQGFGADASSTNEVMISSECGVSKDDIYYSSPGKTEIDIETVLDK
                     CIIIADSITEIELINKISKKYNQKVKIGIRINPDFTMDDDSAKSSKFGIDIEDMSSVF
                     KMLSMCDYVSVVGIHIHIKSQVLDFEKLGHYYEKCFFIALNLNKSKYINIEFINFGSG
                     LGVAYDNLRENPLNLKELSKLTQKIAFTNSNTLKSRLLIESGRFIVCKSGNYYVKIVD
                     IKVSRGKKYLIVKNGLNGFMRPIISNLIENITCGVELEGQEPLYTSNHAFSLSVLNNL
                     KEEEYVNVVGNLCTSLDVIANNVKLKKANIGDLISISNAGSYSYSLSPLLFSSYEFPK
                     QYYKRKDGYIE"
     gene            6445..6642
                     /locus_tag="ACGI59_003378"
     CDS             6445..6642
                     /locus_tag="ACGI59_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HFG9741146.1"
                     /translation="MELWKRNLFVCWIGMFFSSIGMSQIAPILSLYIKQLGVTDVSLI
                     QQYSGIVFGCTFVVAAFFSPI"
     gene            6676..7647
                     /locus_tag="ACGI59_003379"
     CDS             6676..7647
                     /locus_tag="ACGI59_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFG9741147.1"
                     /translation="MLLRASIGMGIVIFTMAFVQNVYQLLGLRILQGVFTGYATACTT
                     LIATQTDKNHSGWALGTLATASTTGSLIGPTVGGYIESILGLKSTFIITGGLLFVSFI
                     ISILFVVDNFKPKEIKESISIPKEQLNILPNKFLIASIFVTTFITQLALYSIEPIVTI
                     YISQLTNFASNVALIAGLTFSASGLANLLAAQKLGKMSDKIGPQKVLLISLLWAGIIF
                     IPQAFVKTAWQLMLLRFLLGLSVAGLNPSVNSLLKKIAPEEYVGKIFGYNASAQYIGC
                     SSGAFLGGQISAHLGIRTVFFSTSLLLFMNALWMYKFTGLFIKDKTK"
     gene            7834..8211
                     /locus_tag="ACGI59_003380"
     CDS             7834..8211
                     /locus_tag="ACGI59_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HFG9741148.1"
                     /translation="MTISKIPQAELKVMKFIWEKNDTVTSKETIEAMERKYGWKQTTT
                     LTLLSRLVKKHFLSAEKIDRYTHYTIVVGHKEYLSVETKDFLSNIHDNSLQSLISALH
                     DDEVVDKDMLDLFESHFKNLEEE"
BASE COUNT         3493 a          893 c         1367 g         2840 t
ORIGIN      
        1 aataaattag agtatttatc aattcatagt agataaaatt gaaattaaat ataacaacaa
       61 aaaatgaaaa ttacaattaa tttaatgtta aagactcaat tatttggatg tggaatggat
      121 agtacaagta gtatataaaa agtattgact ggacaaataa ctatatttta ttttaaatat
      181 ataatataat gtaataaaat atatcaaatg tagaaggtga taacctatga aaaaaataac
      241 aataaatgat attgctaact tggcaggagt atctaaaagt actgtatcaa gatatctaaa
      301 taataaagat atcagtgatt ctactaaaga gaaaataaag acaataatag atgaatatgg
      361 atatgagccg aatgcatttg ctcaaagttt gagagctaaa aaaacatact ttattggtat
      421 tataacaccg tgtttagatt cttttgtaaa atctaaaatt atgatggcaa tagatgaaga
      481 actcaaagaa ttaaaatata catcactaat tattaatact aatagaaaaa taaggtcaga
      541 aatagatagt atatcaaaat tagctagtct aaaagttgat ggaataatac taattggtac
      601 cgaaataact aaagaacata agaatgaaat tgaaaagtta gatataccga tagttgtagt
      661 tggacaaaaa gttgatggta tcaatagcat agtgaatgat gattatggag caggatataa
      721 aatgggtcaa tatattgcta ataaaggtta caaaaacatt gtatatttag gtgttgatga
      781 aagtgatata tcagttggtt taaatagaaa aaatggagta cttaatgggt taaaagataa
      841 agggtatgat gcaaaagtat tttatactga ctttgaccaa gaaacgtcta tacaaagaag
      901 tggagaaatg ctagaaagtg aaaatccaga tataataata tgtgcaactg ataatatagc
      961 aatagcaaca atgaaagaaa tcaataagag aggtaaaaat ataccacaag atatatctgt
     1021 agctggattt gggggatatg atgtattgtc tattataagc cctaaactta caacaataaa
     1081 gtttgaaaat aaaaatgcag gtaaagtagc agcaaatacg atagtaaatt taatacaaga
     1141 aagaaaagaa ccactattaa aggagattaa atttgaatta atagaaggtg aaagtacaat
     1201 aaataaaaat taagatacac attacatata ttagtaatgt gtattttttt actaaaaaaa
     1261 ctataattct aaaaattagg aaaacttttt tgaaagaaat attgcatctg aaataaattt
     1321 atgctaatct ttaattaaac cggtttcgaa accggtttaa ttaaaccgat agaatagcaa
     1381 aatgagtgca aaaataactg attgtaacaa agtatcaaag taattattag agtacatagg
     1441 aataaacaaa atataaaaga tgtagcacat tgtgtaataa gactaagaat tgttttagat
     1501 agcaataata aagcaaatat aaaattataa aagaaaagga ataaaaatat gtacaaagag
     1561 cctaaatata gaaccatatt agaatcgaca aaagaagaat taaataaatt aagaaatata
     1621 tctttaaatg ataaatataa accactattt catatacatc cacaacatgg actacttaat
     1681 gacccaaatg gattagcata ttataatggt aagtatcatg tattttatca atggtatcca
     1741 tatgatgcaa ctcatggaat gaagcattgg gcttatgtaa gttctgatga ttttgtaaac
     1801 tggaatagag aagatgtagc attaatacca atcgaaagtt atgaatctca tggagcatat
     1861 tcaggtaatg cgatagaggt agatggaaaa ttgcatatgt attatacagg gaatataaaa
     1921 tatagtgcag aagatagata tgcatatcaa aacttagcta taatgaacaa ggatggtaaa
     1981 ataactaaat atgaaaataa tccaatagta agtgaaatac caaaaggata tacaggacat
     2041 gtaagagacc caaaagtatt taaaagaaag gataaatact ttatgttact tggagcacaa
     2101 acaagtgata aaaaaggagc tataatagta tatgaatcta aaaactctat agattggaat
     2161 tttaaaggag aattaaatgt aaaaaatata gatgaagatt ttggttacat gtgggaatgt
     2221 ccagattata ttaatataga tgaaaaagat atattaatat tttcaccaca aggagtagaa
     2281 cctaaaggat ttgattatca aaatatatat aatgtagtat atgctattgg taatatggat
     2341 ttagataatt taacatttga aatagataca atgaaagaac tggaaaaagg atttgatttc
     2401 tatgctccac aaacatttat aaaggactca caaataatac tatttgcatg ggctggaatg
     2461 ggagaagttt tataccctac agataaaaac aaatgggcac attgtctaac tgttccaaga
     2521 aaattaaata taaaaaataa taagctattg caaatgccag tagatgaatt aattaaatta
     2581 agatatgatg aaacaagtgg gcaaaatact ataaagaata atattaatat tatagaaaat
     2641 gatgaaaatg tatatgagct taatattaat ataaaaaata ttgacagcaa taaatttggg
     2701 ctggaattat tttcatcaca agatgaaggt gtaaaattag aatttaacaa attaggaaac
     2761 attgtaactt tagacagaag caactttaaa aaagttttcg gtgttgaata tggaacaaat
     2821 agaaaagaat atataaatat tgatgaaaat actaatatca aagtactagc ggatagaagt
     2881 attttagaaa tttttataaa tgaaggagaa gtagtattta ctagtagaat atttgctaaa
     2941 gaaaattcta atcaaataag ggtttatagt gataaaatag tagagtatga gtatactaaa
     3001 tttaaattaa agcaaggtat tgaattataa aaacacatta taaggggtag aaaagatgaa
     3061 aaaagtaatt agcataggag aagctttaat agactttata ccgaatcaaa atggtggtaa
     3121 attaaaaaat gtatcagaat ttagaagagt agcaggagga gctccagcaa atgttagtgc
     3181 tgttgtagca aagcttggag gaaaatcgag ttttatatct aagttaggaa aagatgcatt
     3241 tggtgattat ataatagatg tattaaatga agtaaatgta aatactgatt atgtattgag
     3301 aacaagtaaa gcaaatacag gacttgcttt tgtatcatta aaagaagatg gtaatcgtga
     3361 tttctcattt tatagaaatc caagtgcaga tatgctttta gaagctgatg aagttaaaaa
     3421 agagtggttt aataattgtc atatcctaca tttttgttca gtagatttaa tagatagtcc
     3481 aatgaaatta gcacataaaa aagcaataga atacgcactt gaaagtaata gtataataag
     3541 ctttgaccct aatataagat tacctttatg ggatagtgag caatcttgta aaaaggcaat
     3601 atcagaattt ttaccttttg ctcatattgt taagatatct gatgaagaat tagaatttat
     3661 aacaggagaa aatgatatag aaaaatcatt acataaactc tttgtgggga atgttgaact
     3721 agtattatat acaaaaggaa aagatggagt agatgcatac actaaaaaag taaaaggtat
     3781 gtgtaaaggg gttaaagtaa atgcaataga tacaaccgga gcaggagatt cttatatagg
     3841 ttcattttta tacaccttat tgtataaaca aataacattg aatgatataa aagaaatgaa
     3901 acaagaaacg ttaaatgagt atttagaatt ttctaattat tatgcagctc gtagtacaac
     3961 tattaaaggt gcgataagtt catatgcaac aaaggaagaa ataactgaat ttataaaaaa
     4021 tttatagtag gaatgagtaa taggttttga aggaagtaac atgtaaagag tttttaagga
     4081 tgtttactat gaaaataatt aacatataaa aagcaataaa gaggctgtct caaaatagag
     4141 ataagcctct ttatcattgt tgaaatgata aaaaatattt taattattgt tactttttag
     4201 aagaatatat atcttatttt aaataagcaa attatgtaag atttatttat attatcaata
     4261 ctttacttgg agtattatgt ctttagcagt tatctacttc aattgaatta tctaaaatat
     4321 aataaatttg ttttgcaaat tgacgagaat gttcttcttt ttctttattc ataaaattag
     4381 gtttacctaa atcacgttca acaagcattc ctatatattt taactgtgaa tgcttacagt
     4441 agcgcttcat accattttcc caaatatcag cacccttttc aggtctatat ccacatgttg
     4501 taataagtgc tactggtttt cctgcccata gagtggggcc tttctcatta ccatagtatt
     4561 tattcattcc atacaccaat ctatcaagta gtgctttcat tggagaagtg cagtaccaag
     4621 aataaattgg agtagcaaaa acaattaaat cacacataag aattttcttg aaaattttct
     4681 gaacatcatc tttacaataa catccaaaat caaataagtt atcctgacat tttttacaag
     4741 caatacatgg ctctatttta caatcatata accatattac ttcaatattt gaattataat
     4801 tttctaattc ctcaataaaa ggttttaaaa tagcagcagt attacctttt ttcttaggac
     4861 ttcccataag aatgcatact tccatattga tacctccaca gtattttaaa atagttttta
     4921 gtgcttaata tagttttagg atactatgag aatacgttaa caacaataca ctttgtatat
     4981 ttgtaataaa attgaaacaa attgttttat caataaaagt tggaatgtat ctataattaa
     5041 aaatatttta ggtatgtttt tacatatatg tgtgtagtta actatggtaa tttattgtaa
     5101 aattatctta taggattata attgcttaat ttatgattat atttaaaatc ttggaggtaa
     5161 aaaatatgta ctcaaatgaa gactataaat ttatagaaga aaatgcacca tgttatatat
     5221 atgatgagac taatataatt gaaaaatgta aggaattgaa aaataatatt ttaggttttg
     5281 attttttata ttctataaaa acaaatccat ataacaaaat tgtaaaaagc attgcagagc
     5341 aaggttttgg agcagatgct tcttcaacta atgaagttat gatttcatca gaatgtggag
     5401 tttctaaaga tgatatttat tattcatcac caggcaaaac agagattgat atagaaactg
     5461 ttttagataa atgcataatt attgcagata gtattacaga aatagaactt ataaataaga
     5521 tatctaaaaa gtataatcag aaagttaaaa taggaataag aataaatcct gattttacta
     5581 tggatgatga tagtgctaaa tcaagcaaat ttggtataga tatagaagat atgagcagtg
     5641 tttttaagat gttaagtatg tgtgattatg tatctgtagt tgggattcat atacatatta
     5701 aatctcaggt attggatttt gaaaagttag gacattatta tgaaaaatgt ttttttatag
     5761 ctttaaattt aaataaaagt aagtatatta atattgaatt tataaatttt ggtagtggac
     5821 ttggagtagc ttatgataat cttagagaaa atccacttaa tcttaaagag ctttcaaaat
     5881 taactcaaaa gattgctttt actaattcaa acacactaaa atcaagactt cttattgaat
     5941 caggacgttt catagtttgt aaatcaggta attattatgt taaaattgta gatattaaag
     6001 tatcaagagg gaaaaaatat cttattgtaa aaaatggact taatggattt atgagaccta
     6061 ttatttctaa tttaattgaa aatattactt gtggagtaga attagaaggt caagagccat
     6121 tatatacatc aaatcatgcc ttttctcttt cagtattaaa taatttaaaa gaagaagagt
     6181 atgtaaatgt tgttgggaat ctctgtacat cattagatgt aattgcaaat aatgttaaat
     6241 tgaaaaaagc aaacatagga gatttgattt caattagtaa tgctggaagt tactcctatt
     6301 ctttatcacc gctattattt tcaagttatg aatttcctaa acaatattat aaaagaaaag
     6361 atggatatat tgagtaaaca atagcataaa ttaataaact tagtattatt gaaaaaattt
     6421 aaaatgcagg gggtgtaatt ctatatggaa ttgtggaaaa gaaatttatt tgtatgttgg
     6481 ataggtatgt tttttagttc tatagggatg agccaaatag cacctatact ttcattatat
     6541 attaaacagt tgggggtaac tgatgttagt ttaatacaac agtattcagg tatagttttt
     6601 ggatgtacct ttgtagtagc agcattcttt tcgccaattt gagggaaagc tgctgataaa
     6661 tatggaagaa aatcaatgct tttacgtgca agtattggga tgggaatagt tatatttacc
     6721 atggcgtttg ttcaaaatgt ctatcagtta ttaggattaa gaatacttca aggagttttt
     6781 acgggttatg ctactgcatg tactactctt attgcaacac agacagataa aaatcattca
     6841 ggatgggctt tgggtactct tgcaactgca tcaactactg gctcattaat tggtccaaca
     6901 gtaggaggat atatagaatc aatcttaggt cttaaaagta catttattat aacaggagga
     6961 ttgttgtttg tatcatttat catctctatt ttatttgtag tagataattt taaacctaaa
     7021 gaaataaaag aatcaatttc tatacctaaa gaacaattaa atatattgcc aaataaattt
     7081 ttaattgcat ctatttttgt aaccacattc attactcaac tagctttata ctctatagaa
     7141 ccaattgtaa ccatatatat atcacaactt acaaattttg catcaaatgt agctttaata
     7201 gctggattga cattttctgc atcaggatta gcaaatttat tagcagctca aaaacttgga
     7261 aaaatgtcgg ataaaatagg tcctcaaaaa gtattgttaa tttcactttt atgggctggg
     7321 attatattta taccacaagc ttttgtaaaa actgcatggc aactgatgtt gttgagattt
     7381 ttattagggt tgtcagttgc agggcttaat ccttctgtta acagcttgct taaaaaaatt
     7441 gcaccagaag aatatgtggg aaaaatattt ggatataatg catctgccca gtatataggc
     7501 tgttcatcag gagcattttt agggggacaa atatcagcac atttaggtat tagaacagta
     7561 tttttttcaa caagtttatt gttgtttatg aatgcacttt ggatgtataa atttactgga
     7621 ttgtttatta aagataagac taaataatca tactatttac atttctaatt ttatgatgta
     7681 aaataaatat tttttatgta atttatcata gaattatata aaaaataacc ctatggtaaa
     7741 aattggaaaa tattattatc gacaaaatct ttgacttaaa aactatattg cgatagtatt
     7801 aatttaataa aagtttgaga ggggcgtaag gttatgacaa tttcaaaaat accacaagca
     7861 gaattaaaag ttatgaagtt tatttgggaa aaaaatgata cagtaacatc aaaagaaact
     7921 attgaagcta tggaacgaaa atatggttgg aaacaaacaa ctacattgac acttttatca
     7981 agacttgtta aaaaacattt tttaagtgca gaaaaaatag atagatacac tcattataca
     8041 atagttgttg gacataagga gtatttaagt gttgagacaa aagatttttt aagcaatata
     8101 cacgacaatt ctttacagag tttaatttca gcactacatg atgatgaagt tgttgataaa
     8161 gatatgttgg atttatttga aagtcatttt aaaaatttag aagaagagta attatatcag
     8221 gatagaactc taagaattta attaatctat ggctgaaaac cataaatggt attatttaat
     8281 cagctactca ctattcataa agtaatatta aataggttct ctaattagta aaatgcctaa
     8341 aataattgga atagggagta tcaagttaaa tgataccact tatttaaata agaaatgatt
     8401 tttaaaattt aaaataattt tataacttgt caatattcat taatctaaag tattatttct
     8461 atttaaccat atcaaaatat attttactat ttttaattat tacaatataa acctgtcttt
     8521 tgttattata ggtaaactaa aagtaagaaa tgatttttat atttttaaac tttattattg
     8581 gtaacttatc tgt
//
LOCUS       DAYAPA010000151         8450 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.156, whole genome shotgun sequence.
ACCESSION   DAYAPA010000151 DAYAPA010000000
VERSION     DAYAPA010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8450
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.156"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(200..1069)
                     /locus_tag="ACGI59_003381"
     CDS             complement(200..1069)
                     /locus_tag="ACGI59_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFG9741149.1"
                     /translation="MEYDMPLYRPPSEAYSLIIQVTLGCSHNKCTFCSMYKSKKFKIK
                     PLEVIKNEIDIFRRYYKHVERIFLADGDALIIPMEKLRDIILYIKEVFPECERITMYG
                     SPKSIEKKSDDELKELRKLGVKMIYLGLESGSNEVLEDIKKGFSSEQLIKVGRKVKKS
                     GIKLSATVIAGLGGTKKTHQHAVDTGKMLGAISPDYVGVLSLMVEPNTELYDLLKAGE
                     FTILEDKAVLQEIKEMIKNIDANEKIVFRSNHASNYANLKGVLPDDKQRLIDEIDYYL
                     SNQKLKREEYRRL"
     gene            complement(1166..2023)
                     /locus_tag="ACGI59_003382"
     CDS             complement(1166..2023)
                     /locus_tag="ACGI59_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFG9741150.1"
                     /translation="MSKDVKVKRNKKYDTFSITMELERPLLYDEVENKDDKNNNRKFN
                     IKNKIFKGIGILFVLILLGFFIWINKTYEPQKLAKEALVSNSKVEVTVNENISFTPKG
                     RKVSKGLILYPGAKVEIESYAPLARKIAESGYEVVIVKMPLNLAILGTNKAQKVMDSY
                     NNIEHWVIGGHSLGGVAASNFARDNKLIEGVVFLASYPMGDELKELGKKVISIWGSKD
                     GVVNFKSLVESKQKLPDDTTYVEIEGGNHAQFGDYGKQKGDNDAIISQEKQLNITANS
                     IIKFLKNIS"
     gene            complement(2238..4256)
                     /locus_tag="ACGI59_003383"
     CDS             complement(2238..4256)
                     /locus_tag="ACGI59_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFG9741151.1"
                     /translation="MTKKLVISKTKLFIFIIAFISIFIYLFGSKNTLIGVGIVTAMLT
                     LLERDLTISPIKNLLKYLAINIILGILSFFAVQNMYLGVLLNFIALFIIGYVFSYDLK
                     RAVYVPFGLMYIFMVSIPVGKSEFPMRLSALAVGAVIIMIAQFVMNRNRMKNVGDKEL
                     ISICDELLEKISLLKNTINDDSSIIKSSMRKIDSCNYRINSISKNLKMVIFDNRKDDF
                     YISIRGIDIMNILFSLERISLILERYKKDSKEFEDEDIKNILEESSINSKSDVLVVAT
                     KEINYIKMCLENKDTITDKEILGFRDYVIAQDTKNINLKEIYSVLENLYEFLVDYKKV
                     KSRDEKKAERKSKIPHEFKRLSIYKKNFNLNSIRFSYAVKIALATAVAGFIMDYFHLR
                     DGRWIMLTVFSLTQPYAENCIQRSRKRIEGTFIGAVIFIVLFSIIKDSTLRSLIVLLA
                     GYINSYVVDYRKLMVCVTVSALGSAVVMGDPNVLTISRISYVALGAIIALIVNKFILP
                     YDAKTGYQHVIEMYKGIVKNIIDEVNKSIENVADVYYIKNLLLIPSLIEDRLMLINAI
                     YKDKHQEDFLENQKLLISNMYNLYINVNKNKIKDEDVEKILRDTNYISNYNVDKYDEG
                     RSVILESIINTKSLGDKIICLNLLQTLNGVKEMYRISNITKVSIKEAA"
     gene            complement(4527..5303)
                     /locus_tag="ACGI59_003384"
     CDS             complement(4527..5303)
                     /locus_tag="ACGI59_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFG9741152.1"
                     /translation="MHKETLDKLTNAAINKINLLNTSKVKYLVSSAFAGLYVGIGILL
                     IFTIGGLLTDAGSPMTKIVMGLSFAIALSLVIMTGTELFTGNNMVMSAGMLNKGVSIK
                     DTSKIWAYSWVGNLIGALVLGIIFVGTGLIDKGPVAEFFANTAASKASMPFTALFFRG
                     ILCNILVCVSVLCSFRTNSDTAKIIMIFLCLFAFITSGFEHSVANMTIYSVSLFSPTI
                     NTVTIGGAIYNLVAVTLGNIVGGALFMGLGTYILGKEKLN"
     gene            complement(5360..6331)
                     /gene="asrC"
                     /locus_tag="ACGI59_003385"
     CDS             complement(5360..6331)
                     /gene="asrC"
                     /locus_tag="ACGI59_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016002 - sulfite reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite reductase subunit C"
                     /protein_id="HFG9741153.1"
                     /translation="MIRDLNTKKVMKNAYRITKTKYETSLRVRVPGGLIDPESLAVVS
                     KIASEYGNGQVHITTRQGFEILGINMEDMEEINKIIQPVIEKMNINQSEKNAGYPAAG
                     TRNIAACIGNKVCPKAQYNTTEFAKRIEKAIFPNDLHFKVALTGCPNDCIKARMNDFG
                     IIGMALPIYEKDRCVNCGACVKKCSKISVGALKTENNKVVRDKDKCIGCGECVLNCPT
                     NAWTRDEKKYYRLAIMGRTGKKNPRLAEDFLLWVDEDSIIKIILNTYRYVEKYIDKDA
                     PGGKEHIGYIIDRTGFMEFKKWALEGVELPEITKMYENIYWSGIKYL"
     gene            complement(6344..7135)
                     /gene="asrB"
                     /locus_tag="ACGI59_003386"
     CDS             complement(6344..7135)
                     /gene="asrB"
                     /locus_tag="ACGI59_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016002 - sulfite reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfite reductase subunit AsrB"
                     /protein_id="HFG9741154.1"
                     /translation="MNPYIPVSAKILDIVKHTDKEWTFRVNCNTKGVLPGKFYEISIP
                     KYGESPISVSGYGEDYIDFTIRNVGKVTSELFNYKEGDSFFIRGPYGNGFDVSLYEGR
                     EIVVVAGGSALAPVRGIVEYFYNNKEKCEKFKLIVGFKSPKDILFADDLKRWSKKLDI
                     LVTVDGAEEGYSGNVGLVTKYIPELDMKDINNTSIVVVGPPMMMKFTVEEFLKRDLDE
                     KNIWVSYERKMCCGIGKCGHCKMDDTYICLDGPVFDYSYAKNLID"
     gene            complement(7136..8143)
                     /gene="asrA"
                     /locus_tag="ACGI59_003387"
     CDS             complement(7136..8143)
                     /gene="asrA"
                     /locus_tag="ACGI59_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016002 - sulfite reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfite reductase subunit AsrA"
                     /protein_id="HFG9741155.1"
                     /translation="MKLKLDSSKFNEGLNYLKKDYKIFAPVLIPFKGTFSDTDVIRYK
                     EVNAFEEMEFAKKANFSPKEVVLPINQVLFYFTEKEFKESDLDNKKILIFLRACDING
                     IKRLDEIYLNNGEEKDYFYKNIRDKVKFALVGCKESFRNCFCVSMDSNKTDNYSIGLN
                     IEGDTLYLDIKDSEFEVFNGEATEFNVDYVTDNLISVDVPENIDSNEMIGNSIWDEYD
                     TRCIGCGRCNFVCPTCSCFTMQDIFYKENENVGERRRVWASCQVDGYTDIAGGNSFRK
                     KQGERMRFKTMHKIHDFKKRFGYHMCVGCGRCDDACPQYISFSKCIEKINDLVISKEE
                     V"
BASE COUNT         2727 a         1516 c          765 g         3442 t
ORIGIN      
        1 tttttattat aataggtaaa attctattaa actagttcac attttcatta ttattgatat
       61 attatatatg aatataccat tttgtttttt aataaaaaat catgctacat caatagtaag
      121 caactattaa tgtagcatga ttttttgtat ctaaataatc ttagtattta cctatcagta
      181 tagattgata tatcaaaaac tatagtcttc tatattcttc tctttttagc ttttggttac
      241 ttagatagta atcaatttca tcaattagac gttgtttatc atcaggtaat acacctttta
      301 aattagcata atttgatgca tgattacttc taaatacaat tttttcatta gcatctatat
      361 tctttatcat ctctttaatt tcttgtaaca cagccttatc ctctagtatt gtaaactctc
      421 ctgccttaag taaatcgtaa agttcagtat taggttctac cattaaacta agtacaccca
      481 cataatctgg tgaaattgct cctagcattt ttcctgtatc aactgcatgc tgatgtgttt
      541 ttttagttcc tccaagacct gctattacag tggctgataa tttaatccca ctttttttaa
      601 cttttctacc aacttttatt aattgttcac tactaaaacc tttcttaata tcttctaata
      661 cttcattgct tccactttca agccctagat aaatcatttt aacaccaagt tttcttaatt
      721 ctttaagttc atcatcagat tttttctcta tagatttagg agagccgtac atagtaattc
      781 tttcacactc tggaaaaact tcttttatat ataaaataat atctcttaat ttctccattg
      841 gtattatcaa tgcatctcca tctgctaaga atattctttc tacatgctta taatatcttc
      901 taaatatatc aatttcattt tttattactt ctagtggctt tattttaaat tttttagatt
      961 tgtacatact acaaaacgta catttgttat gagaacatcc tagtgtgact tgtattataa
     1021 gactataagc ctcacttggt ggtctgtaaa gtggcatatc gtattccata ttaaaatccc
     1081 ccttgtaata aaatattcat tctattatat tattaatatt tactttaaat aataacttat
     1141 aaaattatat tttttaaatt ttagtttaag atatattctt taaaaattta atgatactat
     1201 tggcagtaat attaagttgt ttttcttgac ttataatagc atcattatct cccttttgtt
     1261 ttccataatc tccaaattgt gcatgatttc ctccttcaat ctcaacataa gttgtatcat
     1321 caggaagttt ttgttttgat tctacaagac ttttaaaatt gactactcca tctttgctcc
     1381 cccatataga tataactttt tttcctaatt ctttcaactc atcacccatt ggataagaag
     1441 ctaaaaatac aacgccttca attaatttat tatcacgtgc aaaatttgat gctgcaactc
     1501 ctcctaatga atgacctcct attacccaat gttctatgtt gttgtaagaa tccataactt
     1561 tctgtgcctt attagttcca agtatagcta gattaagtgg catctttacg attacaactt
     1621 catatccact ttctgctatt tttcttgcaa gtggagcata agattctatt tctactttag
     1681 ctcctggata caaaattaaa cctttagaaa cttttcttcc ttttggtgta aatgaaatgt
     1741 tttcatttac agttacttca actttactat tgctgactag agcttctttt gccagctttt
     1801 gaggttcata tgttttattt atccatatga aaaagccaag caaaatcaat acaaatagta
     1861 tacctattcc cttaaatatc ttattcttta tgttaaattt tctattatta tttttatcat
     1921 ctttgttctc aacctcatca taaagtaaag gtctctcaag ttccatagtt atactaaatg
     1981 tatcatattt cttattacgc tttaccttta catctttact cataaatcta acccccataa
     2041 tatttgaata ctattatagt ttatcctaag taaattgttt tttcaaccaa tgaaaaaaat
     2101 caatatttta actattttca tataaaattc ataaatatat gttttttgtt ttttatatat
     2161 tattttcttt tagtatttta taaaacaaat agccatatac tttattttaa atatatggct
     2221 attctataat ttaatatcta agctgcttct tttattgaaa ctttagttat attacttatt
     2281 ctatacattt ctttgacacc atttagagtt tgcagtaagt ttagacatat tatcttgtca
     2341 cctagacttt tagtatttat aatactctct aaaattacac ttcttccttc atcatactta
     2401 tctacattgt aatttgaaat ataattagta tctctcaata ttttttcaac atcttcatct
     2461 tttattttat ttttattaac atttatatat agattataca tattacttat tagtaatttc
     2521 tgattttcta aaaaatcctc ttgatgttta tctttatata tagcatttat caacataagt
     2581 ctatcttcaa taagtgatgg tatcaataat aaatttttta tatagtaaac atcagctaca
     2641 ttttctatag acttattgac ttcatcaatt atatttttaa ctattccttt atacatttct
     2701 ataacatgtt gatagcctgt tttagcatca tatggtaata taaacttatt tactattaat
     2761 gcaataattg ctcctaatgc tacatatgaa atcctgctta tggttaaaac atttgggtct
     2821 cccataacta ctgctgaacc aagagctgaa acagttacac ataccattaa ttttctataa
     2881 tcaactacat aagagtttat ataccctgct aaaagaacta ttaacgacct taatgtacta
     2941 tcttttatta ttgaaaataa tacaataaat attacagctc ctataaatgt accttctatt
     3001 ctttttctag acctttgtat acaattttct gcgtaaggtt gtgttaatga aaagacagtt
     3061 aacattatcc atcttccatc tcttaaatgg aaataatcca taataaaacc cgctacagct
     3121 gtagctaaag caatctttac tgcataagaa aatcttatag aatttaaatt aaagtttttc
     3181 ttatatattg atagtcgttt aaattcatga ggtatctttg attttctttc agctttcttt
     3241 tcgtctcttg attttacttt tttataatct actaaaaact catataaatt ttctaaaaca
     3301 ctataaattt cttttaaatt tatattttta gtatcctgag caataacata atctctaaat
     3361 cctaatatct ctttatcagt tatggtatct ttattttcta aacacatctt aatatagttt
     3421 atttctttcg ttgctacaac tagaacatct gatttactat ttatgctact ttcttctaaa
     3481 atatttttta tatcttcatc ttcaaattct ttactatcct ttttgtatct ttctaggatt
     3541 aaacttattc tttctaatga aaataaaata ttcattatgt ctatacctct aatactgata
     3601 taaaagtcat cttttctatt atcaaaaata accattttca aatttttaga tatagaattt
     3661 attctataat tacagctatc tatttttctc atactagatt taattataga actatcatca
     3721 ttaatagtat tttttaaaag actaattttt tctaataatt catcacatat agatatcagt
     3781 tctttatctc cgacattttt catcctattt ctattcataa caaattgagc aatcattatt
     3841 ataactgctc caacagctaa agccgacaat ctcataggaa attcactttt tcctacaggt
     3901 atacttacca taaatatata cattagacca aagggtacat atactgctct ttttaagtca
     3961 taactaaata cataccctat tataaataat gctataaaat ttaataaaac tcctaaatac
     4021 atattttgta ctgcaaaaaa tgatagtatt cctaagatta tatttattgc taaatacttc
     4081 agtaaatttt taattggaga aatagttaaa tctctttcca agagcgttag catagctgtt
     4141 actataccta cacctataag tgtattttta cttccaaata aatatataaa tatagaaata
     4201 aatgctatta taaaaataaa caactttgtt tttgatatta ctagtttctt tgtcatcata
     4261 tcccccctaa aaatttcaac aagaccaatt ttatcatatt tgttatctta ttcaaaatgc
     4321 gcttttttac ggtaggactt taactttttt tatatttaga actatataat tcatatataa
     4381 acgtattttt tatatatata accgatatat tttcgtatgc tcccatattt tttttgtttc
     4441 aaatattttt tcaaaaatat aaaaataact ctctcaacaa tactaaaaat gcatttatca
     4501 agagagttat aatttataca ataaatttaa ttaagctttt ctttacctaa tatataagtt
     4561 ccaagaccca taaataaagc tcctccaaca atatttccaa gtgttacagc tactaaatta
     4621 taaatagctc ctccaatagt aactgtattt attgttggtg aaaataatga aacactataa
     4681 attgtcatat ttgctacact atgctcaaag cctgatgtta taaaagcaaa taaacataag
     4741 aatatcataa ttatttttgc agtatcacta ttagttctaa acgaacacaa cactgataca
     4801 catactagaa tattacataa aatccctcta aagaaaagag ctgtaaatgg catggatgct
     4861 ttactagctg ctgtatttgc aaaaaattca gctacaggtc ctttgtctat tagaccagtt
     4921 cctacaaata taatgcctaa aaccaaagct ccgattaaat tacctaccca actgtatgcc
     4981 catattttag aagtatcttt tatacttaca cctttattta acataccagc agacataacc
     5041 atattgtttc cagtaaataa ctctgttccc gtcattataa ccaaacttag tgctattgca
     5101 aatgataacc ccattactat cttagtcata ggtgaacctg catcagtcaa aagtcctcca
     5161 atagtaaata tcaaaagtat acctatacct acataaagtc ctgcaaacgc agaagatacc
     5221 aaatatttta ctttacttgt gtttaataaa tttatcttat ttatagctgc attagttaat
     5281 ttatccaaag tttctttatg cataattccc tcttccttct attaaatttt aatttattca
     5341 aatatatgtt ttactcaatt tatagatatt taattccact ccagtatatg ttttcgtaca
     5401 ttttagttat ttctggaagc tctacacctt caagtgccca ttttttaaat tccataaatc
     5461 ctgttctatc aattatatat cctatatgtt cttttcctcc tggtgcatct ttatctatat
     5521 acttttctac atatctataa gtattaagta ttatctttat tatgctgtcc tcatctaccc
     5581 ataaaagaaa atcttctgct agtctagggt ttttctttcc tgttctaccc attatagcta
     5641 atctatagta ttttttctca tctctagtcc atgcatttgt tgggcaattt agcacacact
     5701 ctccacagcc tatacactta tctttatctc ttaccacctt attattttct gtttttaatg
     5761 ctccaacaga tatcttagaa cattttttta cacatgctcc acaatttaca catctatcct
     5821 tttcatatat tggtaaagcc ataccaataa ttccaaagtc attcattcta gcttttatac
     5881 aatcatttgg acaaccagtt aaagccactt taaaatgtaa atcatttgga aatatagctt
     5941 tttctattct tttagcaaat tcagttgtat tatattgagc ctttggacag actttatttc
     6001 ctatacaagc tgctatattt cttgtacctg ctgctggata acctgcgttt ttctcacttt
     6061 gatttatatt cattttttca ataacaggtt gtataatttt atttatttct tccatatctt
     6121 ccatatttat tcctaatatt tcaaacccct gtcttgtagt tatgtgcacc tgtccattgc
     6181 cgtactcact tgcaatttta gaaactacag ctaaactctc agggtcaata agacctccag
     6241 gtactctaac tcttaaagaa gtctcatatt ttgtttttgt aattctataa gcatttttca
     6301 ttactttttt ggtatttaaa tctcttatca caaacttcac cccctaatca ataagatttt
     6361 tagcatatga ataatcaaat actggtccat ctaaacatat gtaagtatca tccattttac
     6421 aatgtccaca tttacctatt ccacaacaca tttttctttc ataagaaacc caaatgttct
     6481 tttcatctaa atctcttttt aaaaattctt ctactgtaaa tttcatcatc attggaggtc
     6541 caactacaac tatagaagta ttgtttatat ctttcatatc caattctggt atgtattttg
     6601 ttactaatcc tacattacca ctatatcctt cctctgctcc atctacagta actaaaatat
     6661 caagtttttt actccatctc tttaaatcat ctgcaaataa aatatctttt ggtgatttaa
     6721 atcctactat taatttaaac ttttcgcatt tttctttatt attataaaaa tattcaacta
     6781 ttcctcttac aggggctaat gcacttcctc ctgccactac tacaatttct cttccttcat
     6841 aaagggatac atcaaatcca tttccataag gacctcttat aaaaaaagaa tctccttctt
     6901 tataattaaa caactcgcta gttacttttc ctacatttct tattgtaaag tcaatataat
     6961 cctctccata tcctgatact gatattggac tttcaccgta tttaggtata gatatctcat
     7021 aaaactttcc aggcaataca ccttttgtat tgcagtttac tctaaaagtc cattctttat
     7081 cagtatgttt aactatatct agtatttttg cacttactgg tatatatggg ttcatttata
     7141 cttcctcctt actaattact aaatcattta ttttttctat acactttgaa aatgatatgt
     7201 attgcggaca ggcatcatca catcttccac agccaacaca catatgatat ccgaatcttt
     7261 ttttaaaatc atgtatttta tgcatagttt taaatctcat tctttcgcct tgtttttttc
     7321 taaaagaatt tccacctgct atatctgtat atccatctac ttgacaagaa gcccaaaccc
     7381 ttcttctctc acctacattt tcgttttcct tatagaaaat atcctgcatt gtaaaacatg
     7441 aacatgttgg acatacaaaa ttacatctac cacatcctat acatcttgta tcatattcat
     7501 cccatattga atttcctatc atttcatttg aatcaatatt ctctggcaca tctacagata
     7561 ttaaattatc agttacataa tcaacattaa attcagttgc ttctccatta aatacttcaa
     7621 attcactatc ctttatatct aagtacagtg tatctccttc tatatttaat ccaattgaat
     7681 aattatcagt tttgttagaa tccatgctca cacaaaaaca gtttctaaaa ctttccttac
     7741 accctacaag tgcaaacttt actttatctc tgatattttt ataaaaataa tctttttctt
     7801 caccattatt tagatatatt tcatcaagtc tttttatacc atttatgtca caagctctta
     7861 agaaaattaa aattttcttg ttgtctaaat cgctttcctt aaactctttt tcagtaaaat
     7921 aaaacaatac ttgatttatg ggaagtacaa cttcttttgg tgaaaagttt gctttcttag
     7981 caaattccat ttcttcaaaa gcgtttacct ccttgtatct tataacatca gtatctgaaa
     8041 aagtaccttt aaatgggatt aaaactggtg caaatatctt gtagtctttt tttagataat
     8101 tcagaccttc attgaactta gatgaatcta attttagttt catttttatc acctttctta
     8161 tattttagtt ttacttactt gctatcttgt ttaaatctta acactttatt ttctatgctt
     8221 ccgtgataat tatcacagtt tcaaaaataa taaaaaaaat attaatggag tttattaagt
     8281 tctgttaata tttttttatt atctacatta ttcgatttca aatctaatca cttaaataca
     8341 atcattaagt acaataaaac tactatctac tcaatttttt gaataaaaaa gaatcttatt
     8401 tgttttttta tacaattcaa tagggtaaaa taaaaattaa taaattagac
//
LOCUS       DAYAPA010000152         8331 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.157, whole genome shotgun sequence.
ACCESSION   DAYAPA010000152 DAYAPA010000000
VERSION     DAYAPA010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8331
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.157"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            44..616
                     /locus_tag="ACGI59_003388"
     CDS             44..616
                     /locus_tag="ACGI59_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class B sortase"
                     /protein_id="HFG9741156.1"
                     /translation="MKKLVKILINIICIITLIFSSLSIYIKLSEYKKADEVYTELREN
                     TINNSKHQELYDKNNDYRFWLKINNVNIDYPVVQGYNNDFYLTHDFYKNYLPLGSIFM
                     DYRNNFENDKSLIVYGHYMKNKTMFGQLENYTDEAFFKENNLVEINYKVQTYTYEIFS
                     VYTADLINRDYLSIHFNSNYEFKDYLNYIT"
     gene            complement(1629..2291)
                     /locus_tag="ACGI59_003389"
     CDS             complement(1629..2291)
                     /locus_tag="ACGI59_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9741157.1"
                     /translation="MKMLKVIDIKKIYGKGENEIKAVDGITLEIEPHKFSAIIGQSGS
                     GKSTLLHCMAGLDKPTLGNVFMDNLDLYTLSDEKLSNLRSKEFGFIFQNYNLIPVINV
                     YDNIVLPILIAKGKIDNNYINDLITKLGLESQINKFPNELSGGQQQRVAIARSLANKP
                     SIIFADEPTGNLDSKTTNEVMNLLKYCVNEYKQTLVMITHNDEIAKMADCIITISDGK
                     VI"
     gene            complement(2308..4863)
                     /locus_tag="ACGI59_003390"
     CDS             complement(2308..4863)
                     /locus_tag="ACGI59_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFG9741158.1"
                     /translation="MGIYLKMSLAYLKKNKLRTLLLIIGVMLGVMLIFGPSVIKDSQN
                     ENNVVAIHELYSGYHVEFSDLTLDDAKKLETDEKVSKGNSVQNLGRISDKKGNSFILT
                     SSNEEYLKSRYNKIIEGRLPKNNNEIVLEKKALEAMNLSEGVGSTLNLTIKKNYKDTN
                     GSNQLFSINKEFKIVGVMEKPKGYYDVVWQYEAVTYGNDETNNIIPHDAISYNSILSF
                     KSGWRNIEGECDYLMKKYKLGQKSFLPNVPLVRELRYVEDDKNDPNVFKRNVLITTVS
                     IIFIFNIFNITLNQSVKEMGLLRLMGAKKRNIRCMIIYQALIITIIGILLGLVAGVTY
                     SYIGISNYNEVLYSEAGLNPKLYINNEIVIKAILVGMISVFISCIIPTFKIGKISPIE
                     SIINTDKIKNHRSRLGIDRLVSKIFGFYGFMGLKNIGRNKVRAAISMLSIALGGYIFI
                     TTFSSMQEEVNSKIEDMQNRYNITMQFGVNADPQISKYTDSDVENIEKIKGVKSIHKI
                     QITNGMFTFNNKDINKEFTTYNGVEKKEIMEDKLVLKLYDDDYIDEKLNDFVEEGSLD
                     DIKKDTDGYPNVAVYNYFYDIVDDHTIKPIFKELNIGDIITVKIPVEENNTIVYKEMK
                     VRVAVILKDEWIAMGDGDFMPDLEIITSNIHSKNITGEQKYTQLGINLENPYDEIVNN
                     EIQKISRSIPGSLFNSRLSYHEMSESIYKSYIKSQIAIIALVLIIASINIFCTIRTNL
                     LIRKKEISTLRALGVSLKNMKKMLIYEALAYAVFSFIIAMIPSFINLVKFTNWNNDAY
                     INYGIENFMSFTFPIKESLLFFAISVFVCLISVVVSNRDFESMNIIEGIKENE"
     gene            complement(5103..5291)
                     /locus_tag="ACGI59_003391"
     CDS             complement(5103..5291)
                     /locus_tag="ACGI59_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3139 domain-containing protein"
                     /protein_id="HFG9741159.1"
                     /translation="MRKYKAILLTLIISIIAIIGFIIYNNYFRKWNEAEKYIDKYMTY
                     QGVSGDDIERITKEKYTK"
     gene            complement(5303..5482)
                     /locus_tag="ACGI59_003392"
     CDS             complement(5303..5482)
                     /locus_tag="ACGI59_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9741160.1"
                     /translation="MIHIEISEKEFFRKIEEVFDKGEVNVEVDNLGDIKIQVKKFDDK
                     TKSLELKLIKEFKLK"
     gene            complement(5487..5744)
                     /locus_tag="ACGI59_003393"
     CDS             complement(5487..5744)
                     /locus_tag="ACGI59_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9741161.1"
                     /translation="MSKKISESIENSGLRVAENKSDGSFVLSLGNLEYTKEQPSQVLH
                     YSITKEKKDTKEILSIQCVKDFSYNEKLSEDDKFIMAIYNI"
     gene            complement(6124..6330)
                     /locus_tag="ACGI59_003394"
     CDS             complement(6124..6330)
                     /locus_tag="ACGI59_003394"
                     /inference="COORDINATES: protein motif:HMM:NF025854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFG9741162.1"
                     /translation="MCKYLDNAIEACDKIHDKSIDKYIQIKGTISKSYFVIKCENSKI
                     NSIRKNKSLLLTDKKDKLVHGIGI"
     gene            complement(6506..6751)
                     /locus_tag="ACGI59_003395"
     CDS             complement(6506..6751)
                     /locus_tag="ACGI59_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFG9741163.1"
                     /translation="MIPRLILASSLVIIIVFIKIIKENKLKLQNEVIKNKLDMQYSHY
                     LSIQESHAKVKKLYHDINNHIYCIENLKYKKGKLMGM"
     gene            complement(7391..7714)
                     /locus_tag="ACGI59_003396"
     CDS             complement(7391..7714)
                     /locus_tag="ACGI59_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFG9741164.1"
                     /translation="MKKIKINLTHTKSNLAKKYIEVQKKNMLIHTKNKTLDAICILEK
                     IEKDLNIEELVRCHKSYITNLNYVENIKSNTAILDSQEEVPISRYRYKDIKEGFLKFI
                     GDRIC"
     gene            complement(7953..8105)
                     /locus_tag="ACGI59_003397"
     CDS             complement(7953..8105)
                     /locus_tag="ACGI59_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9741165.1"
                     /translation="MKNLNKNTILGEVIITITACDDEKEIQIVIKNQVDNILKNKNKE
                     YRIKKI"
BASE COUNT         2796 a         1202 c          857 g         3476 t
ORIGIN      
        1 aatttattta attttttata taataatact aaggggtaat taattgaaaa aactagtaaa
       61 aatattaata aatattattt gcattattac gctaattttc tctagccttt cgatttacat
      121 aaaattatct gaatataaaa aagctgatga agtatatacg gaactcagag aaaacactat
      181 caataatagc aaacatcaag aactttatga taaaaataat gattatagat tctggcttaa
      241 aataaacaac gtaaatatag actatcctgt agtacaagga tataacaatg atttctacct
      301 aactcatgat ttttataaaa attatcttcc tttaggtagt attttcatgg attatagaaa
      361 taattttgaa aatgataaga gtttaatagt ctatggtcat tatatgaaaa ataaaactat
      421 gtttggtcag ctagaaaact atactgatga agcatttttc aaagaaaata acttagtaga
      481 aataaattat aaagtacaaa cttatactta tgaaattttt tcagtatata ctgctgactt
      541 aattaacaga gattatttaa gtatacactt taatagtaat tatgagttta aagattattt
      601 gaattatata acctaaaaat cagtacacaa atctgatata aaggtagata tttcagataa
      661 aattataact ttgtatactt gtaactatga atttaaagat gctcgtacta tagttcatgc
      721 taaattaatt tcaaaaaaag agactgataa gtaggtttgt gtaaatgcag acttacctac
      781 agtctttttt gaacgtaaaa ttagtaagta tggaataata ttcatattaa acttttgtag
      841 gcaaagaaat gaaaaatcaa agagaaataa ttattacaga tttagattac caagctgaat
      901 taagaaagtg taaaactatg gaggatgtag ttggtgaaga tagtatataa tagtttaact
      961 tataaattat cttggtgata ttaatgaagt ttcaaagtat atattattta ttgatataaa
     1021 taatattaaa tctgttgcta acagatgtac ctttgtttgg agacaaacta tttaagaaat
     1081 ataactaaaa tgttgaagta gctacctgac tttattttga aatgcgaaca agattttggc
     1141 attaagataa tttagaaaga atcactggta cacaaggatg tgaacttaga atgaatagaa
     1201 gtattcaggc ataaggagct ttcacccaaa taaaacatag tatgaacttt aaaagaattc
     1261 taatcagagg caatcaaaat gttctttcat agtttatacc tgtagtgtta gacatagtat
     1321 ttataagttt cacaaataag ataatcatta ataaatgtag tatgtatatt catcctctca
     1381 aaaatatttt ttaatcttac actttgtaaa ttattctatt ttaaaatggt atttttatac
     1441 ctttatttct tatgaccgtt ttcaagctca aattttgcat aaatataaat taatgaaaag
     1501 caagtaaaaa aagaactgtc gcacagcaat ttaaaattac tcattcgaca gtccttattt
     1561 cgtggattta aatagtggag aagaatcaca agaataatac ccacgattaa acctttatac
     1621 aatacatttt atatgacttt accatcacta atagttatta tacaatcagc catcttagct
     1681 atttcatcat tatgagttat cataaccaac gtctgcttgt attcatttac acaatacttt
     1741 agtaaattca tcacctcatt agttgtttta ctgtctaaat ttccagtagg ttcatctgca
     1801 aaaattattg atggcttatt ggcaagtgat cttgctattg caactctttg ttgttgacca
     1861 cctgataatt cattaggaaa tttattaatc tgactttcta atcctaactt agttattaaa
     1921 tcattaatat aattattatc tatctttcct ttagcaatta aaataggaag aactatatta
     1981 tcataaacat tgataacagg tattagatta tagttttgga atataaaccc aaattcttta
     2041 cttctaagat tagatagctt ttcatcactt aaagtatata aatctaaatt atccataaat
     2101 acattaccta aagttggttt atcaagccct gccatacagt gaagtaatgt gctttttcca
     2161 gaaccacttt gtccaattat tgcactaaat ttatggggtt ctatctctaa agtaattcca
     2221 tcaacagcct ttatttcatt ttctcctttt ccatatattt ttttaatatc tataactttt
     2281 aacatcttca tacatttacc tcccaaatta ttcattctct tttatacctt caataatatt
     2341 catactttca aaatctctat tacttacaac tactgatatt aaacatacaa atacagatat
     2401 agcaaagaat agtaaagatt ccttaattgg gaatgtaaaa gacataaaat tctctattcc
     2461 atagtttata tacgcatcat tattccaatt tgtaaattta actagattta taaatgatgg
     2521 aatcatagct attataaagc taaaaactgc atatgctaat gcttcatata taagcatttt
     2581 tttcatattt ttaagactaa cacctaatgc tctaagcgtt gaaatttctt tttttctaat
     2641 tagtagattt gttcttatag tacagaaaat attaattgat gctattatta aaactagtgc
     2701 aattattgct atttgtgatt taatgtaact tttatatata ctttctgaca tttcatgata
     2761 acttaatcta ctattaaata gagaccctgg tatacttctt gaaattttct gtatctcatt
     2821 attaacaatc tcatcatatg gattttctaa atttatgcct aattgagtgt atttttgttc
     2881 tcctgttatg tttttagagt gaatattact tgttataatt tctagatctg gcataaaatc
     2941 gccatctccc atagcaatcc attcatcctt taaaattaca gccactctta ccttcatctc
     3001 tttataaaca attgtattat tttcttcaac aggaattttt acagttatta tgtctcctat
     3061 atttaattct ttgaatatag gctttatagt atgatcatca actatatcat aaaaataatt
     3121 atatactgct acatttggat atccatcagt gtccttttta atatcatcta agctaccttc
     3181 ttcaacaaag tcatttaact tttcatctat ataatcatca tcataaagct ttaaaactaa
     3241 tttatcttcc attatctctt ttttttctac tccattatag gttgtaaatt ctttgtttat
     3301 atccttatta ttaaaagtaa acattccatt tgttatttga attttatgta tacttttaac
     3361 acccttaatt ttttctatat tctctacatc actatctgtg tattttgata tttgcgggtc
     3421 tgcatttact ccaaattgca ttgttatatt atatctattt tgcatatctt caattttact
     3481 gttgacctct tcttgcatac ttgagaatgt agtaatgaat atatatcctc ctaaagcaat
     3541 agacaacata gatatagcag ctctaacctt atttcttccg atatttttaa gtcccataaa
     3601 tccataaaat ccaaatattt tgcttactag tctgtctatt ccaagtctac ttctgtgatt
     3661 tttaattttg tctgtattta ttattgattc aataggtgat atcttgccga ttttaaaagt
     3721 tggtataata caagatataa aaacagaaat cattccaacc aatatagctt ttataactat
     3781 ttcattattt atatataatt tagggttaag accagcttca ctgtataaaa cttcgttata
     3841 attactaatt cctatatatg aatatgtaac cccagctact aacccaagta gtatacctat
     3901 tattgttata attaatgctt gatagattat catacacctt atgtttcttt tctttgcgcc
     3961 catcagtcta agcagtccca tttcttttac tgattgattt agtgtaatat tgaaaatatt
     4021 aaatatgaaa attatagata ctgtagttat taaaacattt cttttaaaaa cattcggatc
     4081 attcttatca tcttctacat aacgaagttc ccttacaaga ggtacatttg gtagaaaact
     4141 tttttgtccc aacttatatt ttttcataag ataatcacat tcaccttcaa tatttctcca
     4201 tccactttta aaacttaata tactattata actaatcgca tcatgaggta taatgttatt
     4261 tgtttcatca ttgccataag ttactgcttc gtattgccaa accacatcat agtacccttt
     4321 tggtttttcc ataactccta ctattttaaa ttctttattt atagaaaata attgattact
     4381 tccgttagta tctttatagt tcttttttat tgttaaattt aatgtagatc ctactccttc
     4441 acttaaattc attgcttcta gtgctttttt ctctaatact atttcattgt tgtttttagg
     4501 tagtctacct tctattattt tgttgtatct actttttaga tactcttcgt ttgatgaagt
     4561 taatataaat gagtttcctt ttttatctga aatacgtcct agattttgta ccgaattacc
     4621 ctttgatact ttttcatcag tttctaattt tttagcatca tcaagtgtca aatcactaaa
     4681 ctctacatgg tatccactgt aaagttcatg tattgctact acattgtttt cattttgtga
     4741 atcttttata acactaggtc caaatatcaa cataacacct aacataactc ctattattaa
     4801 aagtaatgtt ctaagtttat ttttctttaa atatgctagt gacattttta agtaaatccc
     4861 catataatcc tcctatttta ctataaatta tttatccaac tcatctgtta agtagattgg
     4921 tggatatttt aaatctttta attcttcatt attttttgaa gcttactacc atcttcctca
     4981 tgataaactt gcagtatcac cttatttaca tgtagtgcat aataatcacc attataaaaa
     5041 tattagtatt tataacctgg ataatttttg taaaatgatt tataaagtat cccatcctaa
     5101 ttctattttg tatacttttc ttttgtaata cgctctatat catcacctga aactccttga
     5161 tatgtcatat atttatctat atatttttca gcttcattcc attttctaaa atagttgttg
     5221 tatataataa atcctattat ggctataatg cttataatta aagttaataa aatagctttg
     5281 tattttctca tagtgaatct tcctacttca atttaaattc tttaataagt tttaattcta
     5341 aagacttagt tttatcatca aatttcttaa cttgaatttt tatatctccc aaattatcaa
     5401 cctctacatt tacttcacct ttatcaaata cttcttcaat ttttctaaaa aattctttct
     5461 cacttatttc tatatgaatc aatgatctat atgttataaa tggccattat gaatttatca
     5521 tcttctgaca gcttttcatt ataagaaaaa tctttaacac attgaattga taatatttcc
     5581 ttcgtatcct tcttttcctt agttattgaa taatgtaaaa cttggctagg ttgttctttt
     5641 gtatactcca agtttcccaa agacaaaaca aaacttccat ctgatttatt ttcagctacc
     5701 ctaagaccac tattttctat tgattctgat attttctttg acaagtcttc ttgatactag
     5761 aaatctgttc aatagtatat attttttcat ttgtacctga aaccttattt gtgcatcctg
     5821 ttattaaacc tagtgttaag cttatagcta taaccatagt tattgtaatt tttttatctt
     5881 catataatcc tccttatata ttgaaaactc gttatttctc ataaaaaaaa gacgctatct
     5941 ttttctatta aaagataaca tccctatatg tttagtatca atatatgttc cccaactggt
     6001 aggttaatcg acccaactat caaaattttg atttaatggt atataaatat tcaatataaa
     6061 tttgtctttt ttatcttcaa atgataaatc tcctctatat ttttttatag aagatttgat
     6121 gctttaaatc cctattccat gtacaagttt atctttttta tctgttaata ataaactttt
     6181 attctttcta attgaattta ttttactatt ctcacattta attacgaaat aggactttga
     6241 gatagttcct tttatttgta tatatttatc aatactttta tcatgtatct tatcgcaagc
     6301 ttctattgca ttatctaagt atttgcaaat atattagata catctattgg ttttataaaa
     6361 ttcactttgg aaaaattgat atcacaaatg aatttaattt ttcttttttc acttacatca
     6421 cttttttcat ttattattat atccaatata acattacctg tattgtaaat tgatttaaat
     6481 tctttaattt aatcttttaa actatttaca tacccatcaa ttttcctttt ttatatttga
     6541 gattttctat acaatatata tgattattta tatcatgata aagcttctta actttagcgt
     6601 gtgattcttg aatacttaga taatgagaat actgcatatc taacttattt tttattactt
     6661 cattttgcaa ttttaactta ttttccttaa ttatttttat aaacactata ataatgacta
     6721 agctactagc tagtattaac ctaggtatca caatataccc aactatatat atttcctaat
     6781 acccaccaaa tcgatacatt tttctattta tataaattat taatatagta aataaatttg
     6841 ttattaaagc aacagataag tatatatagt ttattttatt atctttatct agtcccaatc
     6901 ttttattaat aaatttaaat atagcacaag ctactatata actattatta taagataata
     6961 gtatatatct actttagcat tatcataact aaacactaat agagcttcct ctgatgatat
     7021 tacattactt gtaactacaa aaaataaatt tatagatata acatctttta ttaaagggtg
     7081 atatacgtca aataagaaaa atgaaactat atatttaaca attcctatct tataaaaata
     7141 attatagtaa gttcccaagt aataaatatc aaataataga ttatatcata tagatttttc
     7201 ttcatattat aatcatgggt cttatcaaca ttaaataata taatgatctt agttataaga
     7261 taagctaata atacagtaaa aaaagatatt ttcttagtct tatctgtatc tttatcgtaa
     7321 gttaatatac tttttagtat cattattggt gtaaacaatg ataatattaa taatatttta
     7381 tttatatggt ttaacatatt ctatcaccaa taaattttag aaacccctct tttatatctt
     7441 tatatctata tctactaata ggtacttctt cttgactgtc taatattgca gtatttgact
     7501 ttatattttc tacatagttt aagttagtta tataactttt atgacatctt acaagttcct
     7561 ctatatttaa atctttttct attttttcta ggatacatat agcatcaaga gttttatttt
     7621 ttgtatgtat tagcatgttc tttttttgaa cttctatata tttttttgct agattagatt
     7681 ttgtatgtgt tagatttatt tttatttttt tcaatttttt tggtacatgt taatatgtgt
     7741 ttttctagtt cttgaaaatt tattggcttt aataagtatc aataagctct tacttcatat
     7801 ctttcctgta cataatttat tagagatgtt ataaatatta ttatctactt ttcttatgtt
     7861 tcttgcgata tctatactat ttagttctgc catttttata tctaagagta gtgtatcctc
     7921 tcaattaact ttgtaatgat tcttcttttg agctatattt ttttaattcg atattctttg
     7981 tttttatttt ttaatatatt atcaacttga ttttttatta cgatttgtat ttctttttca
     8041 tcatcacaag ctgttattgt aatgataacc tctcctaata ttgtattttt atttagattc
     8101 ttcactttag gttaaaactt tttaaatgta tttatcaata atatttaaat taaaaatgta
     8161 taaaaatacc tatatccaat tggatatagg tattcgtgga tttcaatggt ggagacgagg
     8221 ggagtctata agtggttatt tgattgtttt gttgtttttt gcaagtgtag gtatatagct
     8281 atttagttat gatgttaaac tgattttttc ggatattttt tattgtttat t
//
LOCUS       DAYAPA010000153         8165 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.158, whole genome shotgun sequence.
ACCESSION   DAYAPA010000153 DAYAPA010000000
VERSION     DAYAPA010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8165
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.158"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(105..956)
                     /locus_tag="ACGI59_003398"
     CDS             complement(105..956)
                     /locus_tag="ACGI59_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HFG9741166.1"
                     /translation="MALVTTTSMLKKAQDEGYAVGAFNVENMEMVIAVIEAAEELNSP
                     VILQTTPSTVKYAGLDYYLANAKVAAERVNIPVAMHLDHGSSFDLAMKALRSGYTSIM
                     IDGSHESFENNISISKSVVKACSPSLIPVEAELGKVGGKEDDLDGGNSNDYTNPKEAK
                     EFVDRTGISSLAIAIGTAHGLYKGEPKIDLDRLSEIKNVVSIPLVLHGGSGIHDDIIK
                     EAIKRGIAKVNYATELRIAYSKGVKKVLDEDSEVIDPKKYGLAGLQSVKDFVKEKIKV
                     CGSVNRV"
     gene            complement(1005..2168)
                     /locus_tag="ACGI59_003399"
     CDS             complement(1005..2168)
                     /locus_tag="ACGI59_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFG9741167.1"
                     /translation="MFKLEEQKLKDLGAIITTNEIKQQPELWLETYEIYKSNKEKLSR
                     FIDTISNNHGQFRVIFTGAGTSAYIGNSILPYLKNKNDIRKYIFEAIPTTDIVSNPYD
                     YLKKDIPTLLISFARSGNSPESLAALNLGNKIVDNFYHLAITCNPEGELAKMTKNDEN
                     NYLLLMPSKSNDEGFAMTGSFSCMMLSAMLIFDSLEDDVEKSYINAIIEMGRNVIDRK
                     DEIHELINKDFDRVVYLGSGGLGGLTQEAQLKLLELTAGKISTVYDSPMGFRHGPKSF
                     IDENTLVFEFVSNCLYTRKYDLDVLEEIKRDKIAKFTCAVSVENENNFSGTKFEFKEK
                     YNKLPDVYLAMPYILFAQTIALFVSVKVGNKPDTPSATGTVNRVVKGVTIYEY"
     gene            2645..3562
                     /locus_tag="ACGI59_003400"
     CDS             2645..3562
                     /locus_tag="ACGI59_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFG9741168.1"
                     /translation="MKNIKMGMIGLGSIAQKAYLPILTKSERFEFVGAFTPNKVKREK
                     VCSDYRIMPFDSIESLAKKCDCIFLHSSTETHYEIIKILLNLGVHVYVDKPLASTVSQ
                     GEELIELSTKKNLNLMVGFNRRFCPMYKEIKNNATEIVSINICKHGLNSLRNVRFDST
                     LIDDYIHVIDTALWLANEDVEISGEDLFLTDNKNLIFVSHKLKGKNFSINTSMHRDSG
                     TKLEQVEILSKGKIQRVKNLNVLEIEEGGNLTLKQSGAWVNILKQKGFEDISNHFIDC
                     IENNIKPAINGEECIKAQRLLEKIINSVK"
     gene            complement(3691..4620)
                     /gene="pfkB"
                     /locus_tag="ACGI59_003401"
     CDS             complement(3691..4620)
                     /gene="pfkB"
                     /locus_tag="ACGI59_003401"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFG9741169.1"
                     /translation="MITVITFNPSIDRMYRVNNINIGEVQRVVSKNATAGGKGLNVAK
                     VCKILKENPLAMGFLGGFNGEFIKNELRKLDIQNKFTKIEQETRNCLNIIEDNKVSTE
                     FLEKGPIVEKSDLERFENDIEEVLKNTKILVASGSYCENMPIDYYEKIGNICRANNIK
                     FILDTSGEALKIALKSKPYLIKPNIDEIRHLLGMNIESREEIILSGKKLIEMGAENVC
                     ISLGKDGMVYLNEDNVYDVKVPVVKCINTVGSGDSTVAGFSVGILRGYKIENLLKFAN
                     ACGISNALNMETGFVDLGEIDKYQDLVEVKKLS"
     gene            4889..5614
                     /locus_tag="ACGI59_003402"
     CDS             4889..5614
                     /locus_tag="ACGI59_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFG9741170.1"
                     /translation="MIKRNDKRPIYDQLVEILRTKIENEMEPNDRMLSERKICDEYGV
                     SRTTVRLAMAELEHMGYIYKRHGKGTFVAALSQNSQNLMESYSFTDYMRTQGKKPGTK
                     VLSFEVVESTKYFSEKLGITPGEKMIKIMRLRLADDLPMMLERTYLPMKEFIGLTKEK
                     IEQKPLYEIFREDYGEIIKVADEEFSAGIISDKEAKLLEVPGDSPCLKLLRTTYNDDN
                     IVIEFTLSVARSDKFVYRVRHVR"
     gene            5697..6851
                     /gene="nagA"
                     /locus_tag="ACGI59_003403"
     CDS             5697..6851
                     /gene="nagA"
                     /locus_tag="ACGI59_003403"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFG9741171.1"
                     /translation="MKSFIYADKFFMKHGIKEKGYLSIINGKFGEFQQERPNVTDNVI
                     DFSGKYIAPGLVDTHIHGLLGADVMDNTFEAINTISKGLLKYGVTSFLPTTLTDSVDT
                     LSDSVENISNSFNRVEGAKIQGIYLEGPFFTEKYKGAQNEKYFKDPDINILKLWQKKS
                     KNLIRKIAIAPERKGSIELTRYATKNNISVSLGHSSATFEQVKQVVNSGAKVFVHTYN
                     GMSPLNHREPGMVGAAMVLKDTYAELICDGHHVSPIAVKIMMDAKSSNNIALITDCMR
                     AGAMEDGKYTLGKFNVNVKNSVARLNSGSLAGSVLTMDKAVRNIVDWNISTLEDAIKM
                     ATYVPALSCNIDNVCGSIYQGMDADFIVTDNDFNIHETYINGICKYKVNK"
     gene            complement(7560..8030)
                     /locus_tag="ACGI59_003404"
     CDS             complement(7560..8030)
                     /locus_tag="ACGI59_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HFG9741172.1"
                     /translation="MYNNIKNILKENHITFEEYDHEPILSFEKAKEIREKLQYTGVES
                     KSLFIKDRSGNYYIFVTTEGKKLDPKTIKNVLGGKVSICSKEELTQKTQCLPGCVSPF
                     GYEKNISLVIDQDIFNHDKIIFSPGIPEKTFIITASGFKKVINNINNTTYKYIY"
BASE COUNT         2877 a         1172 c         1031 g         3085 t
ORIGIN      
        1 atcaattaaa tgaaaaaaat acaccattta catataatta atataaattt ttcatataat
       61 aaatatccta aacttatcta aaataaattt aggatatttt atttttatac tctatttaca
      121 gacccacaca ctttaatctt ttctttcaca aaatccttta ctgattgaag ccctgctaat
      181 ccatactttt tagggtctat aacctcagaa tcttcatcta aaactttttt aaccccttta
      241 ctatatgcaa ttcttaattc agttgcatag ttaacttttg caatacctct ttttatagct
      301 tcttttataa tatcatcatg tattcctgaa cctccatgca atactaaagg tattgaaact
      361 acatttttaa tttctgataa cctatctaaa tctattttag gttcaccctt atacaatcca
      421 tgtgcagtac ctatagcaat agccaatgaa gatattcctg ttctatcgac aaactctttt
      481 gcctcttttg gatttgtata atcattacta ttcccgccat ctaaatcatc ttcttttcca
      541 ccaactttcc caagttctgc ttccactggt ataagagaag gactacatgc ttttactact
      601 gatttagata tagatatatt attttcaaag ctttcatgag aaccatctat cattattgat
      661 gtatatccac ttcttaaagc tttcattgca aggtcaaaac ttgaaccatg gtccagatgc
      721 attgctacag gtatatttac tctttctgca gcaacctttg catttgctag ataataatca
      781 agacctgcat attttacagt agatggagta gtttgtaata ttactggaga atttaactcc
      841 tcagcagctt ctataacagc aattaccatt tccatatttt ctacattaaa tgctcctact
      901 gcataccctt catcttgtgc ctttttaagc attgatgttg ttgttactaa tgccacattg
      961 ataccctccc aaattattta gttataatat tttaatctta tcttttaata ttcatatatt
     1021 gttacacctt ttactactcg atttactgtt cctgtagctg atggagtatc tggcttattt
     1081 ccaaccttta ctgatacaaa caatgctata gtttgtgcaa acaatatgta tggcattgca
     1141 agatacacat ctggtaattt attatacttt tctttaaact caaactttgt tcctgagaag
     1201 ttattttcat tttctactga cactgcacaa gtgaatttag caattttatc cctttttatc
     1261 tcctctaaaa catctaaatc atattttctt gtatataaac agttagatac aaattcaaat
     1321 acaagtgtat tttcatcaat aaatgatttt ggtccgtgtc taaaccccat aggagaatca
     1381 tacactgtag atatttttcc tgctgtaagt tctaaaagtt ttagttgtgc ttcttgtgtc
     1441 aaaccaccta atcctcctga acctaaatat acaactctat caaaatcttt atttattaac
     1501 tcatgtattt catcttttct atctataaca tttcttccca tttcaattat tgcatttata
     1561 tatgactttt ctacatcatc ttctaaacta tcgaatatta acatagcaga taacatcata
     1621 cacgaaaaac ttcctgtcat ggcaaagcct tcatcatttg attttgatgg cattaataac
     1681 aagtaattat tttcgtcgtt tttagtcatc ttagccagtt ctccttctgg attacaagtt
     1741 atagctaaat gataaaaatt atctacaatc ttattcccta aatttaaagc tgctaaactt
     1801 tcaggactat ttccacttct tgcaaatgaa attaataatg ttggtatatc tttttttaag
     1861 taatcatatg ggtttgaaac tatatccgta gtaggtattg cttcaaatat gtattttctt
     1921 atgtcatttt tatttttaag gtaaggaagt attgaatttc ctatgtaagc tgaagttcca
     1981 gcacctgtaa atataactct aaactgccca tgattattac ttattgtatc tatgaatcta
     2041 cttaattttt ctttgttaga tttatatatt tcataagttt ctaaccataa ttcaggctgt
     2101 tgttttattt cattagttgt tattattgca cctaaatctt ttagtttttg ttcttctaat
     2161 ttaaacattt tcattctcct ttcatacttg taacgtttac atttggtaat gaggttataa
     2221 ccactttaaa ctataaaaaa atatactctt ttataatttt ttgcttagat tttttagtac
     2281 tacttaaact cactataaaa tatacactat agattttaat taattttata gtttatcttt
     2341 aaaaatttaa taaaatcata ctctatatac tttagtattt ttattttacg ataaaagctt
     2401 gttcagacca aagtggtaat taccactaat ccaaatataa attaatttga attttttgtc
     2461 aatacataaa atatttatat caatcaattt tatggatatc ctacaaaatt actttatcaa
     2521 tatttcttat ttcgctaatt tacttatata agctttaaat ttattttttg atacaaagtt
     2581 tacaaattta tgtgtctgaa ttataatcaa ttctgtaaat ataaaaacct taggagaaag
     2641 tattatgaaa aatataaaaa tgggaatgat aggtcttggc tccatagctc aaaaggcata
     2701 tttacctata ttaactaagt ctgagagatt tgagtttgta ggagctttta ctccaaataa
     2761 agttaaaagg gaaaaagtct gtagtgatta tagaatcatg ccattcgatt ctattgaatc
     2821 cctagctaaa aaatgtgatt gtatttttct acatagctct acagaaacac actacgaaat
     2881 tataaaaata ttattaaatt taggtgttca tgtatatgta gacaaacctc ttgcttcaac
     2941 agtaagtcaa ggagaagaac ttatcgagtt aagtacgaaa aaaaacttaa atttaatggt
     3001 tggattcaac cgtagattct gtcctatgta caaagaaatt aaaaataatg ctacagaaat
     3061 tgtatctatc aatatatgta aacatggatt aaattcctta agaaatgtta gattcgacag
     3121 tactcttata gatgactata tacatgttat tgacactgct ttatggttag caaatgaaga
     3181 tgttgaaatt agtggagagg atttattttt aacagacaat aagaatctta tatttgtaag
     3241 tcataaactt aaaggtaaaa atttctctat aaacacctct atgcatagag attctggaac
     3301 aaagttagaa caagttgaaa ttctttcaaa gggtaagatt caaagagtta agaatcttaa
     3361 tgtactggaa atagaagaag gtggaaacct aactttaaag caaagtggtg catgggtaaa
     3421 tatattaaaa caaaagggtt ttgaggatat atcaaatcat tttattgatt gtatagaaaa
     3481 taatataaaa ccagctatta atggagaaga gtgtatcaaa gctcaaagat tactagagaa
     3541 aattataaat tcagtaaaat agatatttat tataaaaata ggttaaaaat gaatttttct
     3601 aacctatttt tatataagac tatatctttt atattaaaat taaaatctat tacaacaaca
     3661 ttaaatttat tatattccca ttaaatgtat ttaacttaac ttttttactt caactaaatc
     3721 ttgatattta tcaatttctc ccaaatctac aaatccagtt tccatattta aagcattact
     3781 tataccacaa gcatttgcaa atttaagtaa attttctatt ttataacccc ttaaaatacc
     3841 tacactaaat ccagccactg tgctatctcc actacctaca gtatttatac actttacaac
     3901 tggtactttt acatcatata cattatcttc gtttaaatac accataccat cttttcctaa
     3961 agatatacat acattctctg cacccatttc aataagtttt tttcctgaca agataatttc
     4021 ctctcttgat tctatattca ttcctaataa atgtcttatt tcatctatat ttggttttat
     4081 taaatatggt tttgatttta aagctatttt taatgcttct cctgatgtat ctaatataaa
     4141 ctttatatta tttgctctac atatatttcc tatcttttca taatagtcta tcggcatatt
     4201 ctcacaataa ctaccagaag ctaccagtat tttagtattt tttagtactt cctctatatc
     4261 attttcaaat ctctctaaat cgcttttttc tactattggt cctttttcta aaaattctgt
     4321 actaacttta ttgtcctcaa ttatgtttaa gcaatttcta gtttcctgct ctatttttgt
     4381 aaatttattt tgtatatcta gttttctaag ttcatttttt ataaattctc cattaaatcc
     4441 gcctagaaat cccatagcta aaggattttc ttttaaaatt ttacatacct tagctacgtt
     4501 taatccttta cctccagcag ttgcattctt acttactacc ctttgcacct cacctatgtt
     4561 tatattattc actctataca ttctatctat agatgggttg aatgttatta ctgtaatcat
     4621 actagattct cctcattatt aatttttact tttctaaaca ataaattaat ctgtaaaata
     4681 aaaaatacct catctataat atacccaaag tggttattac caatttgaat ataaattatt
     4741 ttacttgttt tgtcaatgta ccataatttt ataataaatt aataacctta cttattgaat
     4801 tttgattaaa ctttattcat tgacataaat aattctatat tctataatca aattgtggta
     4861 ataaccactt agaaaggact atatttttat gataaaaaga aatgataaaa gaccaatata
     4921 tgaccagctt gttgagatat taagaactaa gattgaaaat gagatggaac caaacgatag
     4981 aatgctttcc gaaagaaaaa tttgtgatga atatggagta agtagaacca cagttagact
     5041 agctatggct gagttggaac atatggggta tatatacaaa agacatggaa aaggaacttt
     5101 tgttgctgca ctaagtcaaa attctcaaaa cttaatggaa agttatagtt ttacagatta
     5161 tatgagaaca caaggaaaaa agccaggtac aaaggtatta tcatttgagg tagtagaaag
     5221 tacaaagtat ttctctgaaa aattaggtat aactcctggt gaaaaaatga taaaaataat
     5281 gcgtttaaga cttgctgatg accttcctat gatgcttgaa agaacatatc ttcctatgaa
     5341 agaatttata ggattgacaa aagaaaaaat cgaacaaaaa cctttatatg aaatatttag
     5401 agaagattat ggcgagatta ttaaagttgc tgatgaagaa ttttcagctg gaataatctc
     5461 tgataaggaa gctaagttat tggaagtacc aggagattca ccttgtctga aattacttcg
     5521 taccacatat aatgatgaca atatagttat tgaatttact ttgagtgttg ctagaagcga
     5581 taaatttgta tatagagtaa gacatgtaag atagtaagtt aaataacttt caagaaaata
     5641 gaatgatatt ataaacgtca atgccacaat gatagtataa ttataaggag gttattttga
     5701 aaagttttat ttatgcagat aaatttttta tgaaacatgg aatcaaagaa aaaggttacc
     5761 ttagtataat taatggcaaa ttcggtgaat ttcaacaaga aagacctaat gttactgata
     5821 atgttataga tttttcaggt aaatatattg ctcctggctt agttgatact cacattcacg
     5881 gtcttttagg tgcagatgtc atggacaata catttgaagc aataaacaca atttcaaaag
     5941 gcttattaaa atatggagta acatctttcc tacctacaac tttaactgat tctgtagata
     6001 cactaagtga ttctgtagaa aatataagca atagcttcaa tcgagttgag ggtgcaaaaa
     6061 tacaaggtat ttatttagaa ggtccatttt ttacagaaaa atataagggc gctcaaaatg
     6121 aaaaatattt taaagaccct gacatcaata tattaaaatt atggcaaaaa aaatcgaaaa
     6181 atttaataag aaaaatagca attgctccag aacgtaaagg ttcaatagaa ttaacaagat
     6241 atgcaactaa aaataatata tcagtttctc ttggtcatag ttcagctaca tttgagcaag
     6301 ttaaacaagt cgttaattca ggtgctaaag tgtttgtaca tacatataat gggatgagtc
     6361 ctttaaatca tagagaacct ggaatggttg gggctgctat ggtactaaaa gatacttatg
     6421 ctgaattaat ttgtgatggt catcatgtaa gcccaatagc tgttaaaata atgatggatg
     6481 cgaagtctag taataacata gctttaatta cagattgtat gagagctggt gctatggagg
     6541 atggaaaata tactcttggt aagtttaatg taaatgtaaa aaatagtgtt gcaagattaa
     6601 atagtgggag tttagctgga agtgtattaa ctatggacaa ggctgtgaga aatatagttg
     6661 attggaacat atcaacttta gaagatgcaa taaaaatggc tacatatgtc cctgctttaa
     6721 gttgtaatat tgataatgtc tgtggctcta tatatcaagg tatggatgca gactttatag
     6781 taacagataa tgactttaat atacacgaaa cttatataaa tggtatctgt aagtacaaag
     6841 ttaataaata ggttcaatat aaggttatgg aggctgtcta aaaagagtct ggaaatattt
     6901 aaagttattt ctccaaaatc tgatttaaca cttttatttc catatcgtta ataaactatt
     6961 gcgatagagg ttattttaaa caaatatata ttataagtat ttttgtgata atattttcga
     7021 taatatgtac atttaaagta acttttctat tattatttgt aagtttttat cttgagcaaa
     7081 catataaatt tttaataaag catgagataa accctatatt tcaattacta atgatgagat
     7141 tttgattgat aacagatata ttttcaaatg taaaaatact aaaaatcata tcaattttaa
     7201 agatattgta ggtgtaaata aacaagataa tactatagaa atttttacaa atgaaagcac
     7261 ttattgtata tatctaaaag ttttaagcat tgagagcgaa gaatatctaa tagatagact
     7321 aaaatcattt tgttgatttt atatatttta tgttaaatat aaagcagagt agttaaaact
     7381 accctgcttt tgtaattaaa atttaaaaat aataactcat aatacattga atttgagctt
     7441 aacagtaact attgtaataa tagtatcaat cttttgtgtt gaatataatt tatttacact
     7501 atagttaagc atgatttcta atgttatttt ctctaattta caattatgat taaaattcat
     7561 taatatatat atttataagt tgtattgttt atattgttta ttactttttt aaatccacta
     7621 gctgtaataa taaatgtctt ttctggtatc cctggtgaaa agattatttt atcatggtta
     7681 aatatatctt gatctattac aagagagata tttttctcat atccaaaagg agatacacat
     7741 ccaggcaaac attgtgtttt ttgtgttaat tcttcttttg aacatataga tacttttcca
     7801 cctaaaacat tttttatagt ttttggatct aatttttttc cttctgtggt tacaaatata
     7861 tagtaatttc cagatctatc ttttataaat aaacttttac tttctactcc agtatattgt
     7921 aatttttctc ttatttcttt agctttttca aatgataaaa taggttcatg gtcatattct
     7981 tcaaacgtta tatgattttc ttttaaaata tttttgatat tgttgtacat atattttcct
     8041 ttctatattt aaattaattt atatataata ttcaattata aagtgatttt ataatttctt
     8101 caaactatca taaaaaacat tcacatgttt taggaataaa ttatataaaa taggttataa
     8161 gataa
//
LOCUS       DAYAPA010000154         8135 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.159, whole genome shotgun sequence.
ACCESSION   DAYAPA010000154 DAYAPA010000000
VERSION     DAYAPA010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8135
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.159"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            158..868
                     /locus_tag="ACGI59_003405"
     CDS             158..868
                     /locus_tag="ACGI59_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFG9741173.1"
                     /translation="MINIGICDDELHYRIKIKDILSEILSSYPINYNIYEFSAGEELL
                     NNYPKDLDILIMDIQMKTINGMDTARKIREFDHKLEIIFVTSFVEFMQEGYEVKAYRY
                     ILKPINKEKISKSVLPCINEMMKKRNNYLTINVKNYVDRIKIDSITYIETDRPNILIY
                     THDDMYITKMSISKIEKILNEYGFFRCHNSYIVNLKLVESMSGSTVIVDGKSIPISKY
                     RVKGLKLAITNILGDIVC"
     gene            862..2178
                     /locus_tag="ACGI59_003406"
     CDS             862..2178
                     /locus_tag="ACGI59_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFG9741174.1"
                     /translation="MLTNLSVGFWNIINLVSGTIDWIIFFLVIHLVGKRKISNIRYIF
                     TTIIIIIFMAYINVVKVFPNIKILLCMVIGISFYITSYEDKIYKSIIVSLLFWLGLMV
                     AEGSAVGLVVFINKLNNINITQHENLFRIQAIIMSKVFLFIGFILFKYFKLSLDFKPK
                     DMILIGIPILSNIVSLLLIFEYNLRVNTITTENLFIFIFAILLILSSSIILLIVIGKI
                     VQDDKLNLEYELINERIKTNYRNYENINEVHDRLKYVYHDLKNHMICIKNYDTKEEII
                     SYINNLEFQISEFEHFKNTGNKTLDLILAEKISICKKYDIQIEDNINISKLNFIKNND
                     ICSIFANSLDNAIEACMDINNEIEKRIEVKATYINKFAIIKFINTKVNDIKLIDKRIQ
                     TSKDDDKIHGIGLASIKYIVNKYGGETIVNYSDNEFILKIMIPIKS"
     gene            2190..2330
                     /locus_tag="ACGI59_003407"
     CDS             2190..2330
                     /locus_tag="ACGI59_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="HFG9741175.1"
                     /translation="MKKIALNLLKNISALSFGIAVLSANSASSWVAHQAKEPQALQKL
                     KK"
     gene            2389..2970
                     /locus_tag="ACGI59_003408"
     CDS             2389..2970
                     /locus_tag="ACGI59_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator ArgB-like protein"
                     /protein_id="HFG9741176.1"
                     /translation="MFKRLSYKFANILVNNEIVESEDFEIYRYGFETLIYFIVNILVA
                     LFIGIIFDRFIHTVIFLSCYCTLRQFTGGYHARNYKECTLTFAVIYLITIFSANNIDI
                     NKYKYLLVLLMIISILTIYKLAPLEHRNKPLSESEKKHYRKTVQKILFVIICLIILCK
                     ILNIFQQYVIYAIISIYWIAILIYIGMKVNSDQ"
     gene            3117..4826
                     /locus_tag="ACGI59_003409"
     CDS             3117..4826
                     /locus_tag="ACGI59_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9741177.1"
                     /translation="MSSIKAISDFITKIIRKNILFAITIIIINYHLIQRWTHISNRGI
                     SFNDYLSLSVYTCMTISILTMLNVIFYMRKSYDTVKILCRNNFDYFLGISVAVTRNNF
                     IFCCIPIIYAIAYSSLIRTLSIVTIKSTIFILIFEWFLPLTILSIVISFISVIINNLI
                     LKITLSSVFLYLTSTNMLDRVIDIKEPFFKALFKSLNIFEDQSYAYYSDFVGKVNNSA
                     YVIDKIIPTLFAITLIIVAYLILNDIRKKQIKSLIVIVVYSILFIGLNYISIDMSTYR
                     DEERSYGEYLKKSNISDIKIKSHDMKIDFKSKSNIKDRIVLLNTSNEELTNINMILDE
                     IFDIKYIKINSENIKFNKKKDLIEVNLKESLEPNDEVTLDIEYEGYINVLTSWNQNKY
                     IATKDDIMLPPGSLAWYPKVNQSSCMAYKINLSSKNNVYSNLPLLNKTSNFLKKDYIF
                     EGKSNDIAIYAGEFKEEVKDDIKIIYPSDSNVNMDIEEKIKFLIETRNEILGDSKASV
                     DAKKYVDEVDNVMESIKRKKINMILVADLPSNEFLYEDSKGEKISITIYHWLSGNILI
                     IDK"
     gene            4841..5713
                     /locus_tag="ACGI59_003410"
     CDS             4841..5713
                     /locus_tag="ACGI59_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFG9741178.1"
                     /translation="MINKLEIRNLNFNYKNKKALDNINLTLIDGVVALLGPNGAGKST
                     LMRLLVTLYETSIGEIELNNIKYSKNNEKIKANVGYVPQDFDMYNNINGQEYLEFVAK
                     MRGVSKNDLKKHIQKVVSKVNLDKFINKKIGTYSGGVKRRLGIAQALIGDSKLIVMDE
                     PTVGLDPEQRNEFRRLLPEISKNSIVLISTHIVEDIQFNCDKLIILNQGKILYDGTIN
                     KFIDMVEVYSVVVSNDEFEYLERNIDIIDYRRVKDGVQVKYIGKNTEKLKDSLRIDTT
                     LQDAYINFLHKELK"
     gene            5714..6502
                     /locus_tag="ACGI59_003411"
     CDS             5714..6502
                     /locus_tag="ACGI59_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9741179.1"
                     /translation="MCILELIKFELKKIELKHVLIFVIISILCIFITGILDYNELIFY
                     NDVPIMDTYSIAGWLGIIKISDVFIPIMSAITIIYVFSKDYSGNINEILTLYNKKKYN
                     QFIVFRWCVILAIYTVLLLISITIVYTFTSVKGFNIDKIDFVYSVKPIDVFLKTFPTL
                     LWYTTFPLMILTLTKNRFTTIAILITYTFADIFFILHMYPFVSMINVNGFYIQKMFYM
                     PNNIDVRFNELKNYFFINRGSLVVISIIIVSYIARKSTVLKKSK"
     gene            complement(6598..7431)
                     /locus_tag="ACGI59_003412"
     CDS             complement(6598..7431)
                     /locus_tag="ACGI59_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HFG9741180.1"
                     /translation="MNVENQSLSDIEIILSHRYDNGADYWTTPDKRLIKGSPFSTLDS
                     VLYLLELGVNPDDTLLKECADLIFSTWREDGRFKLYPNGGIYPCHTANAANVLCHMGY
                     ASDIRMQKTFQHFLDTQYTDGGWRCNKFSFGRGPETEYSNPLPTLNILNAFRFSNYLN
                     KESKLDKAVDFLLDHWTIKKPIGPCHYGIGTLFMQVEYPFRNYNLFLYVYVLSFYNCA
                     KKDNRFLEALKILESKMIGNKIVVERVVPKLSKLSFCKKGESSEIATTHYYEILKNLE
                     K"
     gene            complement(7499..>8135)
                     /locus_tag="ACGI59_003413"
     CDS             complement(7499..>8135)
                     /locus_tag="ACGI59_003413"
                     /inference="COORDINATES: protein motif:HMM:NF024067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3786 domain-containing protein"
                     /protein_id="HFG9741181.1"
                     /translation="KIMDEKITKNNYNVPFDYVRKKLKELDPHTISNLSNIPFNNDLN
                     RFTLNFFDRQYIIDYPSGEVWNSNGSLCNNFELKILIVRYLINAKGIPRTNKYVTFKE
                     IPGGNVYYPNFLNRTLYRLSEVFTSQIDKYKLVMEKIGAEKVNMGDLAYRFTYMGNTS
                     MVFILWFGDDEFPPNSNILFDSNIVYYFNAEDLAVVPDTAINAILNKINQL"
BASE COUNT         3284 a          794 c         1153 g         2904 t
ORIGIN      
        1 aaatagattg caaatccaaa aagtaatact taatgtccaa aaagctaaaa ttaaaatcta
       61 aatagctatt tactacagaa gtatgttaaa atatgtaaaa tgaatagaaa tttatatatt
      121 tttattaaaa atatataaga aaggatgttt aagaattgtg attaatatag gaatatgtga
      181 tgatgaacta cattatcgaa taaaaataaa agatatatta agtgaaatat taagttcata
      241 tcctataaat tataacatat atgaattttc agcaggagaa gagttactaa ataactatcc
      301 taaagattta gacatattaa ttatggacat acaaatgaaa acaataaatg gtatggatac
      361 tgctaggaag ataagagagt ttgatcataa gttagaaata atatttgtta cttcatttgt
      421 agagtttatg caagaaggtt atgaggtaaa agcatacaga tatatactaa agccaataaa
      481 taaagaaaaa atttctaaaa gtgtattacc ttgtataaat gagatgatga aaaagagaaa
      541 taattactta actataaatg taaaaaatta tgtagaccga attaaaatag attctattac
      601 ttatatagaa acagatagac cgaatatatt gatttataca catgatgata tgtatattac
      661 taaaatgagt atatctaaaa tagaaaagat attaaatgaa tatggatttt ttagatgtca
      721 taatagctat atagttaact taaaattagt agaatcaatg agtggaagta ctgttatagt
      781 agatggaaaa agtattccaa taagtaaata tagggttaaa ggcttgaagc ttgcgataac
      841 taatatatta ggggacatag tatgctaaca aatttaagtg taggattttg gaatattatt
      901 aatttagttt caggtactat agattggatt atattttttt tagtaatcca cttagttgga
      961 aagaggaaaa tatctaatat aagatatata tttacaacaa taattataat tatatttatg
     1021 gcatacataa atgtagttaa agtgtttcca aatattaaga ttcttctatg tatggtaata
     1081 ggaatatcat tttatataac atcatatgaa gataaaatct ataaaagtat aatagttagt
     1141 ttattgtttt ggctaggatt aatggttgct gaaggttctg cagtaggtct agtagtattt
     1201 ataaataaat taaataatat taacataaca caacatgaaa acttatttag aatacaagct
     1261 attattatgt caaaggtatt tttatttata ggttttatat tgtttaagta ttttaaatta
     1321 tctttagatt ttaaacctaa agatatgata ctaataggaa tacctatatt atctaatata
     1381 gttagcttat tacttatatt tgaatataat ttaagagtca atacaattac tacagaaaac
     1441 ttatttatat ttatatttgc aattttattg attttatcat caagtataat acttttaata
     1501 gtaataggga aaattgtaca agacgataaa ttaaatctag agtatgaact tataaatgaa
     1561 agaataaaaa caaactacag gaattatgaa aatataaacg aagttcatga tagattaaaa
     1621 tatgtttatc atgacttgaa aaaccatatg atttgtataa aaaattatga cactaaagaa
     1681 gaaattatat catatataaa taacttagag tttcagataa gtgaatttga acattttaaa
     1741 aatactggaa ataaaacatt agatttaatt ttagcagaaa aaataagtat ttgtaaaaag
     1801 tatgatattc aaattgaaga taacataaat atatctaaat taaactttat taaaaataat
     1861 gatatatgtt caatatttgc aaattcacta gataatgcaa ttgaagcatg tatggatata
     1921 aataatgaga tagaaaaaag aatagaagtt aaagctacat atataaataa atttgcaata
     1981 ataaaattta taaatactaa ggtaaatgat attaaactta ttgataaacg tattcaaact
     2041 agtaaggatg atgataagat acatggaata ggacttgcta gtattaaata tatagtaaat
     2101 aaatatggtg gtgaaacaat agttaattac tcagataatg agtttatatt aaaaataatg
     2161 ataccaataa aaagttagga gagtacaaaa tgaaaaaaat agcgttaaac ttacttaaaa
     2221 acatctcagc attaagtttt gggatagctg tattatcagc aaacagtgca agttcttggg
     2281 tagcacatca ggcaaaagag ccacaagctt tacaaaaact aaaaaaataa cattaataaa
     2341 aggacctctt cttattaaag gagaggtttt acatgttgag gaggaaatat gtttaaaaga
     2401 ttatcatata aatttgccaa tatattagtt aataatgaga ttgtagaaag tgaagatttt
     2461 gaaatatata gatatggatt tgaaactttg atttatttca tagttaatat tttggtagca
     2521 ttatttatag gaattatttt tgatagattt atacatacag ttatattttt aagttgttat
     2581 tgcactttaa gacagttcac aggaggttat catgctagga attataaaga gtgtacatta
     2641 acatttgcag ttatttattt gataactatt ttttcagcta ataatataga tataaataaa
     2701 tataagtatt tattagtatt attaatgatt attagtatac taacaattta caagttagct
     2761 cctttagaac atagaaacaa acctcttagt gaaagtgaga aaaagcatta tagaaagact
     2821 gtgcaaaaaa tactattcgt aataatatgt ttaattattt tatgtaaaat acttaatatt
     2881 tttcagcaat atgtgattta tgcaataatt tctatatatt ggattgctat attaatatat
     2941 attggaatga aggttaatag tgatcagtaa gattataaaa cattttctaa attaattaac
     3001 ttcactaatt aatattaact ttaaaaaaat aatgtcaagg taagtatttg aacttattaa
     3061 atatagttga atctattgta ggttgtatca aaagactggc taggaggggc ataaatatga
     3121 gtagtattaa ggctatatca gactttataa ccaaaataat tagaaaaaat attttatttg
     3181 caataacaat tattattata aattaccatc tcatacaacg atggacacat atatccaata
     3241 gaggaatttc atttaatgat tacttatcct tgagtgtata tacttgtatg acaattagta
     3301 ttttaaccat gttaaatgta attttttata tgagaaaaag ttatgatact gtaaaaatat
     3361 tatgtagaaa taattttgat tattttttag gtatttctgt tgctgtaact agaaacaact
     3421 ttatattttg ttgcatacca attatatatg ctatagcata ctcaagttta ataagaacat
     3481 tgtcaattgt aacgattaaa tcaactatat ttatattgat atttgaatgg tttttaccac
     3541 ttactatttt atctatagtg atatcattta tttcagttat tataaataat ttaatactaa
     3601 agattacctt atcatcagta tttttatatt taacatcaac taatatgttg gatagagtta
     3661 ttgatattaa agaaccattt tttaaagcat tatttaaatc acttaacata tttgaagatc
     3721 aatcatatgc ttactattct gattttgtag ggaaagttaa taattcagca tatgttatag
     3781 ataagattat acctacacta tttgcaatta ctttaataat agtagcatat ctgatattaa
     3841 atgatattag aaagaaacag attaaatcat taatagttat agtagtgtac agtattttat
     3901 ttattggatt aaattatata tcaatagata tgtcaacata tagagatgag gagcgtagtt
     3961 atggagagta tttaaagaaa tctaatatta gtgatattaa aataaaatcg catgatatga
     4021 agattgactt taaaagcaag tcaaatataa aagatagaat tgttttatta aacacatcaa
     4081 atgaagagtt aacaaatatt aatatgattt tagatgaaat atttgatata aaatatataa
     4141 aaataaattc ggaaaatata aaatttaaca aaaaaaagga tttaattgaa gttaatttaa
     4201 aggaaagttt agagcctaat gacgaggtga cattagatat agagtatgaa ggctatatta
     4261 atgtgttgac atcttggaat caaaataaat atatagctac taaagatgat attatgcttc
     4321 caccaggttc tttagcgtgg tacccaaaag taaatcaatc aagttgtatg gcttataaaa
     4381 taaatttaag ttctaaaaat aatgtctatt caaatttacc tttactgaat aagactagta
     4441 attttttaaa aaaagattat atatttgaag gaaagtcaaa tgatattgca atatatgcag
     4501 gtgagtttaa agaagaggta aaagatgata taaaaataat ctatccatca gatagtaatg
     4561 taaatatgga tatagaggaa aaaattaaat ttttaattga aacaagaaat gaaatattag
     4621 gtgatagcaa agcatctgta gatgctaaaa aatatgtaga tgaagtagat aatgttatgg
     4681 aatcaataaa aagaaaaaaa attaatatga tattagttgc tgacttacct agcaatgaat
     4741 ttttatatga agactcaaag ggagagaaaa ttagcataac tatttatcat tggctttcag
     4801 gaaatatcct tataatagat aaataataaa ggaggttaat atgataaaca agttagaaat
     4861 aagaaatcta aattttaatt acaaaaataa aaaagctttg gataatataa atttaaccct
     4921 aatagatgga gtagttgcat tattaggtcc taatggtgct ggaaaatcaa cattaatgag
     4981 gttattagtg actctttatg aaacttcaat tggagagata gaattaaata atataaagta
     5041 tagcaaaaac aatgagaaaa tcaaggcaaa tgtaggatat gtacctcaag attttgatat
     5101 gtataataat ataaatgggc aagaatactt agaatttgta gctaaaatga gaggtgtatc
     5161 taagaatgat ttaaagaaac atatacaaaa agtagtatca aaagttaatc tggacaagtt
     5221 tataaataaa aaaataggta catactcagg tggtgtaaag agacgactag gtatagcaca
     5281 ggctttaata ggtgattcta agttaatagt tatggacgaa cctacagttg gtcttgatcc
     5341 agagcaaagg aatgaattta gaagattgtt accagaaata agtaaaaatt ctatagtatt
     5401 aatttcaact cacatagtag aagacataca atttaactgt gacaaattaa ttatattaaa
     5461 tcaaggcaaa atattatatg atggtacaat taataagttt attgatatgg ttgaggtgta
     5521 ttcggtagta gtttctaatg atgaatttga atatttagaa cgtaatattg atattattga
     5581 ttataggaga gttaaagatg gtgtgcaagt aaagtatata ggtaaaaata cagagaaact
     5641 taaagatagc ttaaggatag atacaacttt acaagatgct tatataaact ttttacataa
     5701 ggagttaaaa taaatgtgta tattggagtt aataaaattt gagcttaaaa aaatagagct
     5761 taaacatgtg ttaatatttg taattatttc tatcttatgc atatttatta caggcatatt
     5821 agattataat gagttaatat tttataatga tgtacctata atggatacat attctatagc
     5881 aggatggtta ggaattataa agatatcaga tgtttttata ccaataatgt cagcaatcac
     5941 aataatttat gtgttttcaa aagattattc gggtaatata aatgagatac ttactttata
     6001 taataaaaaa aaatataatc aatttatagt atttagatgg tgtgttatat tagctatata
     6061 tactgtatta ttattaattt caattacaat agtatataca tttacgtcag tgaaaggttt
     6121 taacatagac aaaatagatt ttgtttatag tgtaaagcca atagatgtgt ttttaaagac
     6181 atttcctaca ttattatggt atacaacatt tccactaatg atattaacat taacaaaaaa
     6241 tagatttaca acaatagcaa tattaattac atatacattt gctgatatat tttttatttt
     6301 acatatgtac ccatttgtta gtatgattaa tgtaaatgga ttttatattc aaaagatgtt
     6361 ttatatgcct aacaatatag atgttagatt taatgaatta aagaattatt tctttataaa
     6421 tagaggttct ctagtagtta tttctataat aatagtttct tatatagcaa gaaaatcaac
     6481 ggttttaaaa aagagtaaat agtttaatat attttttata atgttatatt tatatagaat
     6541 agtgacttta aaaaatttaa agccactatt ttttaatatg ttttatctat ataactatta
     6601 tttttctagg ttttttaata tttcgtaata gtgtgttgtg gctatttcac ttgactcgcc
     6661 ttttttacaa aaagataatt tagatagttt tggaactaca cgttcaacga caattttatt
     6721 acctatcatt ttagattcaa gtatcttcaa tgcttctaaa aatctattat ccttttttgc
     6781 acaattataa aatgataaaa cgtagacata aaggaatagg ttataattac ggaaaggata
     6841 ttcaacttgc ataaataagg ttccaattcc atagtgacaa gggccgattg gttttttaat
     6901 agtccaatgg tcaagtagaa aatctacagc tttgtcaagt tttgattctt tatttagata
     6961 attactaaaa cggaatgcat ttaatatatt aagagttggt agtggatttg agtattcagt
     7021 ttctggacct cgaccaaaac taaatttatt gcaacgccac ccaccatcag tatactgtgt
     7081 atctaaaaaa tgttgaaacg ttttttgcat tctaatgtca gatgcatatc ccatatgaca
     7141 gagtacgtta gcagcatttg cagtatgaca agggtaaata cctccatttg gatatagttt
     7201 gaaacgtcca tcttctcgcc atgtgctaaa tatcaagtca gcacattctt taagtaaagt
     7261 atcatcaggg ttcacaccta attctaatag ataaagtaca ctatcaagag tagagaaagg
     7321 agaacctttt attaaacgct tatcaggtgt agtccaatag tctgcaccat tatcatacct
     7381 gtgtgataat attatttcaa tatctgataa agattgattt tctacattca ttttttactc
     7441 catttcatat tatattctat taaatctatc aaaaacttta ttataatatt atttatattt
     7501 ataattgatt tattttattt aaaattgcat ttatagctgt atcaggaaca actgctaaat
     7561 cttctgcatt gaagtaataa actatattag aatcaaataa tatatttgaa tttggaggaa
     7621 attcatcatc tccaaaccaa agtataaata ccatagatgt attacccata tatgtaaacc
     7681 tataagctaa atctcccatg tttacttttt cagctccaat tttctccata actaacttat
     7741 atttgtctat ttgagatgta aaaacttctg aaagcctata tagagtcctg tttaaaaaat
     7801 ttggataata aacattgcct cctggtattt ctttaaaagt tacatattta ttagttctag
     7861 gaataccttt agcattgatt aaatatctaa ctatcaagat tttaagctca aagttattgc
     7921 aaaggctacc attactattc caaacttctc cacttggata gtctataata tattgtctat
     7981 caaaaaaatt gagtgtgaat ctatttaaat cattattgaa aggtatatta cttaaattac
     8041 taattgtatg agggtctaat tctttgagtt tttttcgaac atagtcaaat ggaacattat
     8101 aattattttt tgttatcttt tcatccataa ttttt
//
LOCUS       DAYAPA010000155         8025 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.160, whole genome shotgun sequence.
ACCESSION   DAYAPA010000155 DAYAPA010000000
VERSION     DAYAPA010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8025
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.160"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            169..936
                     /locus_tag="ACGI59_003414"
     CDS             169..936
                     /locus_tag="ACGI59_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaP domain-containing protein"
                     /protein_id="HFG9741182.1"
                     /translation="MQIIDININNIDNEHICCSISEKKGENCVFSKKAWMKNNFDKGL
                     VFKRFDVRGKAFIEYIPAENAWCPITADNYIFINCFWISGKFKGQGYSNLLLQECVDD
                     AKSKGKYGLVVLSSSKKMPFLSDAKYLKYKDFCLADTSEPNYELLYFPFTDNAPIPYF
                     KNCVKKRKLNENGIVLYYSNQCPHTEKYAPILKKIANSRGIDVNLIKFETAKEAQNAP
                     APFTTYSLFFNGEFVTNEILSEKKIEKFLDTNIKKFN"
     misc_binding    1154..1367
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1621..2655
                     /gene="argC"
                     /locus_tag="ACGI59_003415"
     CDS             1621..2655
                     /gene="argC"
                     /locus_tag="ACGI59_003415"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HFG9741183.1"
                     /translation="MNKIKVGIIGATGYVGAELIRLLMNHDKVEVTAIGSNSYVGKDI
                     VDIYPSIGYKNNMICIENEKVIDMCDVVFTALPHGVSEKFVIKAIKSKKKVIDLGADF
                     RIKDEEVYSKWYGVSFIDKILHKKAVYGLSEIYKEDIKDADIIANPGCYPTSISLPLM
                     PLLSSKLIKNNNIIIDSKSGLTGAGRELSISSHFTEVNENITAYKIGKHRHTPEIEQN
                     LSESCKEKVSVVFTPNLIPVNRGILSTIYCTKEDNISINDIHRKLTDYYEFKEFIEIL
                     PLDKVASLKNVRFSNKCVISLHENGDTLIICSAIDNMIKGAAGQAIQNMNIIFGIEEN
                     TGLKNIAPSF"
     gene            2696..3913
                     /gene="argJ"
                     /locus_tag="ACGI59_003416"
     CDS             2696..3913
                     /gene="argJ"
                     /locus_tag="ACGI59_003416"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HFG9741184.1"
                     /translation="MEIIKGGVTVSEGFFASGIHCGLRKNKEKRDLALVYSDVLCDAA
                     AVVTQNKVKGNPVYVTQEHIKNGKAQAIIVNSANANTFNGKEGLINAYKMAKFTSDKL
                     NIKESDVLVASTGVIGKPLNIDLIEENMDELVNNLSKQGHIGAREAIMTTDIIKKEIA
                     VAIMYGDKKITIGGMAKGSGMIHPNMATTLGFITTDANIDGVLLKEALKIAVDKSFNR
                     VSVDGDTSTNDMVMILANGKAKNDRINKKDEHYQVFLSALTYVCIELAKLVAKDGEGC
                     TKLIECYINGALSEEHAVKLAKTVISSSLVKTAVFGADANWGRILCALGYAGEEIDME
                     KVDIIFESMKGYIEVCKNGNGLDFNEEKAKKILEDDEISILVDLNMGNARGNAWGCDL
                     SYDYVRINGSYRN"
     gene            3928..4788
                     /gene="argB"
                     /locus_tag="ACGI59_003417"
     CDS             3928..4788
                     /gene="argB"
                     /locus_tag="ACGI59_003417"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HFG9741185.1"
                     /translation="MINIEKANTLIEALPYIEKHQGKTIVVKYGGSAMKKDGLKESVM
                     EDLVLMSYVGINIVLVHGGGAEINKMLAKVDIESKFVNGLRYTDEETMEIVKMVLAGK
                     VNKDLVNKIHTKGGKAVGLCGIDNNMILCDPYKNYELGFVGEIKKVNVELIESCLKSG
                     YISVIATIGVGDDGETYNINGDTAASAIAKELNADKLILLTDVPGLLREPDEEKSLIT
                     EVILEDVDKLFEEGIITGGMIPKIEGCVDALNNGVNRVHILDGRVPHSIITELFTDSG
                     IGTLIRKETE"
     gene            4818..6005
                     /locus_tag="ACGI59_003418"
     CDS             4818..6005
                     /locus_tag="ACGI59_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HFG9741186.1"
                     /translation="MNNDNTISKWNEYFIDTYNQPNFVIDYGEGSCFFDTNGNKYIDF
                     TSGYGVSSLGYSNSNLKNALKEQVDKLLHTSNLYFNEPVLYSGEKIINSSGMAKVYFC
                     NSGTEANETAFKIARKYSSDKYGNGRGTIISLKDSFHGRTMMSLMATGMDKYHKYFYP
                     LPEGFKYIERNNIEDLKNNLDSTVCAIILEAIQGEGGVNVLEKDYVLEIVKICQEKDI
                     VVIFDEVQCGIGRTGKLFGYEYFDVKPDIVTVAKGLGAGIPVGGVLVNKKLSKVLGKG
                     DQGTTFGGNLLAMVAASVVLDEISKDGFYNEVLEKGNYIRKSIESFNNKVILKTKGIG
                     LMIGIETNIESSIIEEKARKKGLLILTAGKNVLRFLPPLTISYKEIDEALEILKDILL
                     EIN"
     gene            6114..7136
                     /gene="argF"
                     /locus_tag="ACGI59_003419"
     CDS             6114..7136
                     /gene="argF"
                     /locus_tag="ACGI59_003419"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFG9741187.1"
                     /translation="MNNISLKGKSFLTLKDFTKEEIRYLLTLSSYLKTKKKVGIKGDL
                     LQGKNIALLFEKTSTRTRCSFEVAAHDEGAHVTYLGPNDCHMGKKESVSDTAKVLGRF
                     YDGIEFRGFKQETVESLAKYSGVPVWNGLTDEYHPTQILADFLTVIENVDKDLNKVKF
                     VYVGDARNNMGNSLMIGCAKMGIHFVALAPKELWPNEELVKEMKEIAKKSKGDIMLTE
                     NIDDVKGADVIYTDVWVSMGEEEQYEARINQLKDYQVNMDMIKKTENENVIFLHCLPA
                     FHDLETKVAQEIYEKFGLSELEVTNEVFNSKYSKVFEEAENRMHTIKAVMVSTMTDVN
                     MNNNLI"
     gene            7263..7880
                     /locus_tag="ACGI59_003420"
     CDS             7263..7880
                     /locus_tag="ACGI59_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4878 domain-containing protein"
                     /protein_id="HFG9741188.1"
                     /translation="MKKIILALTTFLLAISLTACSSAKPEDTIDSFFNSAKKFDFEGM
                     NKVMENNDEKYKDILKELDTKDPNAQYVLDYLKQNASKITYTIKDSEVKGDKATIKVE
                     CKFIDSTPLLQEIVAEAFTKMIGMSFSGQDLTDEKTTEMLVSIMKEKQKSVKETYVTK
                     NVEFECSKKDNKWIISSVNDAVADVLLSNLVTAGQEFSNSMNTLE"
BASE COUNT         3272 a          756 c         1491 g         2506 t
ORIGIN      
        1 atagaatgta tttaattata tatacaattt aataattcag ataatcttaa aataatatta
       61 tgtaaaataa aaatatatat gacatgatgt tgttgtattc aaaatattaa gataaaaata
      121 tatagaatat atttaaaaat tattatgcaa ttagaaaagg agataagtat gcagattatt
      181 gatataaata taaataatat tgacaatgaa catatatgtt gttcaatttc tgaaaaaaaa
      241 ggagaaaatt gtgttttttc taaaaaagct tggatgaaaa ataattttga taaaggtcta
      301 gtctttaaac gatttgatgt aagaggtaaa gcttttatag aatatattcc agcagaaaat
      361 gcatggtgtc ctattactgc ggacaattat atatttatta actgtttttg gatatctgga
      421 aaatttaagg gtcaaggata ttctaatcta ttgctacaag agtgtgttga tgatgcaaaa
      481 tcaaaaggta aatatggatt agttgtgctt tcgtctagta aaaaaatgcc ttttttatct
      541 gatgctaaat atctaaaata taaggacttt tgtttagcag atacatcaga accaaattat
      601 gagctattat attttccttt tactgataat gctcctattc catattttaa gaattgtgta
      661 aaaaaaagaa aattaaatga gaacggtata gttttatatt acagtaatca gtgtccacat
      721 acggaaaaat atgctcctat tttgaaaaaa attgcaaatt ctagaggtat tgatgtaaat
      781 cttataaaat tcgaaacagc aaaagaagca caaaatgctc ctgcaccatt tactacatac
      841 agtctttttt ttaatggtga atttgtaacc aacgagatac tttctgagaa aaagattgag
      901 aaatttttag ataccaacat taaaaaattt aactaaaaag agatgtaaat attgtatttt
      961 gaaaatatat tattttataa tttaatatat tttggatgtt ttttaaattg acgaatgaaa
     1021 tataaaaatt atatcgcaat ttagatacaa aatgctggaa attaaccata tagaaaatat
     1081 ttccaaaaat ttaaaaatta tattgacaaa gttaaaaaaa taaactatca tataaaacaa
     1141 taagaaaata aattgaaatc ctgtgaaaaa gagagtagca taaagctagt tgatagagag
     1201 ctgggaatgg tggaagccta gtaacgaatt ttattgtgaa tagagctttg gaggagatta
     1261 ccagaagtaa tagtatggta attggtgaat ctcgccttaa gagattagag tgggtaagat
     1321 tatcttatca attagagtgg taacgcgggt gtatattcgt ctcttggtct ttgaccaaga
     1381 ggctttttta attttaaatg tacattttgt agatactttt taatttgagt tttaatgatt
     1441 attgtaattt taccaataag agtggtaccg cggaaaatcg cctcttagta tttataatgc
     1501 taaggggttt tttgttttaa ttagagtatt ttaatctacg tattatcaaa tgattatcat
     1561 atagtaaagt ggaaagatag gattttaaga ctatttgaat ataaaaatgg agggaacaaa
     1621 atgaataaaa taaaagttgg tattattggt gcaactggat atgtcggggc agagttgatt
     1681 agattattaa tgaatcatga taaagtcgaa gtgacagcga taggttcaaa ttcgtatgtt
     1741 ggaaaagata tagttgatat atatccaagt ataggatata aaaataacat gatatgtata
     1801 gaaaatgaaa aagttataga tatgtgtgat gttgtattta cagcccttcc acatggagtt
     1861 agtgaaaagt ttgtaataaa ggcaattaaa tcgaaaaaga aagttataga tttaggtgct
     1921 gactttagaa taaaagatga agaagtatat agtaaatggt atggagtatc ttttatagat
     1981 aaaatcttac ataaaaaagc tgtatatgga ttaagtgaaa tatacaaaga agatataaaa
     2041 gatgcagata taattgcaaa tcctggatgt tatccaacta gtatttcttt accattgatg
     2101 ccattactaa gttctaaatt gataaaaaat aataatataa taattgattc taaatcaggt
     2161 cttacaggtg ctggtagaga attatcgatt agtagtcact ttacggaggt taatgaaaat
     2221 atcactgcat ataaaatagg gaagcacaga catactccag aaatagaaca aaatttaagt
     2281 gaatcctgca aagaaaaagt aagtgtagtt tttacaccaa acttaatccc agtaaatagg
     2341 ggaatcttat caactatata ttgtacaaaa gaagataata tttctataaa tgatatacat
     2401 aggaaattaa ctgattatta tgaatttaaa gaatttatag aaattcttcc attagataag
     2461 gtagcttctt taaagaatgt tagattctca aataaatgtg taatatcatt acatgaaaat
     2521 ggagatacat tgataatctg ttcagctata gataatatga ttaagggagc agcaggtcaa
     2581 gctatacaaa acatgaatat aatatttggt attgaagaaa atacaggttt aaaaaatatt
     2641 gcaccatcat tttagaaata aaaaatacta aactcatata attaggagga taaatatgga
     2701 gattataaag ggtggagtta cagtttcaga aggatttttt gcttcaggga ttcactgtgg
     2761 cttgagaaaa aataaagaaa aaagagattt agctttggtt tattcagatg ttttatgtga
     2821 tgcagcagca gtagtaactc aaaataaagt aaagggcaat cctgtttatg ttacacagga
     2881 acacattaaa aatggaaaag cacaagctat catagtaaat agtgctaatg ctaatacctt
     2941 taatggaaaa gaaggactta taaatgctta taaaatggct aaattcacca gtgataaatt
     3001 aaatattaaa gaaagtgatg ttttggttgc atctacaggt gttataggaa agccattaaa
     3061 tatagattta atagaagaaa atatggatga attagtgaat aatctaagta aacaaggtca
     3121 tattggagca agagaagcta ttatgacaac tgatattata aaaaaagaaa tagcagtagc
     3181 aataatgtat ggagataaaa aaataactat tggaggtatg gcaaaaggtt ctggcatgat
     3241 acatcctaat atggcaacaa cgttaggatt tataactaca gatgcaaata ttgatggagt
     3301 tttgctaaaa gaagctttaa aaattgctgt tgacaaaagt tttaatagag ttagtgtaga
     3361 tggagataca agtactaatg atatggtaat gattctagca aatggaaaag caaaaaatga
     3421 tagaataaac aaaaaagatg aacattatca agtatttttg agtgcactta catatgtctg
     3481 tatagaactt gctaaacttg ttgcaaaaga tggagaagga tgtacaaaac taattgaatg
     3541 ctatataaat ggagcattaa gtgaagaaca tgcagtaaaa ttagcaaaaa cagttatatc
     3601 ttcttcatta gtaaagacag ctgtatttgg agctgacgca aattggggta gaatactgtg
     3661 tgctttaggt tatgcaggtg aagaaataga tatggaaaaa gttgatatta tttttgagag
     3721 tatgaaagga tatattgaag tatgtaaaaa tggaaatgga ttagatttta atgaggaaaa
     3781 agcaaaaaaa atccttgaag atgacgaaat aagtatatta gtagatttaa atatgggcaa
     3841 tgccagagga aatgcatggg gatgtgattt aagctatgat tatgtaagaa taaatggaag
     3901 ttatagaaat taggaggata tactttaatg attaatatag aaaaagcaaa tacacttata
     3961 gaagctttac catatataga aaaacatcag ggaaagacta ttgtagttaa gtatggtgga
     4021 agtgcaatga aaaaagatgg tctaaaagaa tctgttatgg aagaccttgt gttaatgagt
     4081 tatgttggaa tcaatatagt tttagtacat ggtggagggg cagaaatcaa taagatgcta
     4141 gcaaaagtgg atatagaaag taaatttgta aatggtctta gatatacaga tgaagagacc
     4201 atggaaattg taaaaatggt acttgctgga aaggttaata aagacttagt aaataaaata
     4261 catacaaaag gtggaaaagc agtagggctt tgtggaatag ataataatat gattttatgt
     4321 gacccatata aaaattatga acttggattt gtaggagaaa tcaaaaaagt aaatgttgag
     4381 cttatagaat cttgtctaaa gtcaggttat atttcagtaa ttgcaactat aggtgttgga
     4441 gatgatggag agacttataa tataaatggt gacacagcag cttctgctat agctaaagag
     4501 ttaaatgcag ataaattgat acttcttaca gatgtccctg gtttgttaag agagccagat
     4561 gaagaaaaat cattaataac agaagttata ttagaggatg ttgataaatt atttgaagaa
     4621 ggtataatta ctggaggtat gatacctaaa atagagggat gcgtagatgc cttaaataac
     4681 ggagtaaata gggtacatat tttggatgga agagttccac attctataat aacagagcta
     4741 tttactgata gtggaatagg tacattaata agaaaagaaa ctgaataaaa ttataagttt
     4801 aagaggggag aataagaatg aataacgaca atacaatttc aaaatggaat gaatatttta
     4861 tagatacata taaccaaccc aattttgtca tagattatgg tgaaggaagt tgtttttttg
     4921 atactaatgg caataaatat atagatttta caagtggata tggagtatct tctcttgggt
     4981 atagtaatag taatttaaag aatgctctaa aagagcaagt agataaatta ttacatactt
     5041 caaatttata ttttaatgag ccagtgttgt attcaggaga gaaaataatt aattcaagtg
     5101 gaatggctaa agtatatttt tgtaattctg gaacagaagc aaatgaaaca gcattcaaaa
     5161 tagcaagaaa gtatagtagt gacaaatatg gtaatggtag aggaactatc ataagtttaa
     5221 aagactcttt tcatggtaga acaatgatgt ctttaatggc aactggtatg gacaaatacc
     5281 ataagtattt ttatcctctt cccgaaggtt ttaaatatat agaaagaaat aatattgaag
     5341 atttaaaaaa taatcttgat tccacagttt gtgcaataat tcttgaagcg attcaaggcg
     5401 aaggtggagt taatgttctt gaaaaagatt atgttttgga aatagtaaaa atatgtcaag
     5461 aaaaggatat tgtagtgatt tttgatgaag tccaatgcgg tataggaaga actggtaaac
     5521 tttttggcta tgaatatttt gatgtaaaac cagatatagt taccgtagca aaagggttgg
     5581 gagcaggaat accagtagga ggagtattgg taaataaaaa actttcaaaa gtactaggaa
     5641 aaggagacca aggtacaact tttggaggta atttattagc tatggtggca gcttcagttg
     5701 tattagatga gatatctaaa gatggctttt ataatgaagt attagaaaaa ggaaattata
     5761 taagaaaatc tattgaaagt tttaataaca aagttatatt aaagacaaaa ggtattggtc
     5821 taatgatagg aattgaaact aatattgaat ctagtattat agaagagaaa gctagaaaaa
     5881 aaggattgct tattttgact gctggaaaaa atgtattgag atttttacca ccattaacaa
     5941 tcagttataa ggaaatagat gaggctcttg aaatcctaaa agatatatta ctagaaatta
     6001 actaaatatt caaaaaatag ttgattaaag ataaaaatta gaagtaaata aaaaagtaaa
     6061 gtgctaacaa gtttaattga atttaaaaag tttataagaa ggggcgaatt aatatgaata
     6121 atataagttt aaaaggtaag agttttttga cattaaagga ttttacaaaa gaagagatta
     6181 gatatttatt aactttatca agttatttaa aaactaagaa aaaagttggt ataaaaggcg
     6241 atttgctaca aggaaaaaat atagctttat tatttgagaa aacatcaaca agaactagat
     6301 gttcatttga agtagcagct catgatgaag gagcacatgt aacttatcta ggtccaaatg
     6361 attgtcatat gggtaaaaaa gagtctgtat cagatacagc aaaagttcta ggaagattct
     6421 atgatggaat agaatttaga ggctttaaac aagaaacagt tgaaagtcta gcaaaatatt
     6481 ctggtgtacc tgtttggaat ggacttacag atgaatatca tccaacacaa atactggcag
     6541 attttttaac agtaattgaa aatgtagata aagatttgaa taaagttaaa tttgtatatg
     6601 ttggtgatgc tagaaataat atggggaact cactgatgat aggatgtgct aaaatgggaa
     6661 tacatttcgt agcactagca cctaaggaac tttggccaaa tgaagaattg gttaaggaaa
     6721 tgaaggaaat agccaagaaa tccaaaggag atataatgtt gactgaaaat attgatgatg
     6781 ttaaaggtgc agatgttata tatacagatg tatgggtttc tatgggagaa gaggagcaat
     6841 atgaagcaag aataaatcaa cttaaagatt atcaagtaaa tatggatatg ataaagaaaa
     6901 ctgaaaatga aaatgttata tttttacact gtcttccagc atttcatgat ttagaaacta
     6961 aggttgctca ggagatttat gaaaagttcg gattatcaga attagaggtt acaaacgagg
     7021 tatttaattc aaaatattca aaggtatttg aagaagctga aaatagaatg catacaataa
     7081 aagctgttat ggtttctact atgactgatg taaatatgaa caataattta atatagatat
     7141 gttttaatat agactttact ttaatgaaaa tttataaaat attttaaata tattattcaa
     7201 aaactctcat tatatgttaa aataacttat gtatcaacaa attaagaggg aggatttgag
     7261 atatgaaaaa aatcatatta gctttaacaa cttttttatt agcaatatct ttaacagcat
     7321 gttcaagtgc aaaaccagaa gatacaattg atagtttttt taacagtgcg aaaaagtttg
     7381 actttgaagg catgaataag gtaatggaaa ataatgacga aaaatataag gatatattaa
     7441 aggaattaga tacaaaagac ccaaatgccc aatatgtttt agattatcta aaacagaatg
     7501 catctaaaat cacatataca ataaaagaca gcgaggttaa aggtgataag gctacaatca
     7561 aagtagaatg taaatttata gattcaacac cattacttca agaaatagta gctgaggcat
     7621 ttacaaagat gattggtatg agtttttcag gacaagactt aactgatgag aaaacaactg
     7681 aaatgttagt atcaattatg aaagaaaaac aaaaaagcgt taaagagact tatgttacaa
     7741 aaaatgtaga gtttgaatgt tctaaaaagg ataataaatg gataataagt tcagtaaatg
     7801 atgcagtagc tgatgtattg ttatctaatc ttgttacagc aggacaagaa ttttctaact
     7861 ctatgaatac acttgaataa tattgtttag gtgtagtaaa gtaataaata tatgattatt
     7921 tttataaata aacatataaa tatttatgaa ggataccatt tttggtatcc tttttattaa
     7981 tataatgaaa tacatggtta atttctatat tgtcaagaaa ctatg
//
LOCUS       DAYAPA010000156         8022 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.161, whole genome shotgun sequence.
ACCESSION   DAYAPA010000156 DAYAPA010000000
VERSION     DAYAPA010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8022
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.161"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(226..1296)
                     /gene="leuB"
                     /locus_tag="ACGI59_003421"
     CDS             complement(226..1296)
                     /gene="leuB"
                     /locus_tag="ACGI59_003421"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HFG9741189.1"
                     /translation="MNCNIAVIKGDGVGPEIIDEGIKVLNKICCKFNHRFDCEYVLAG
                     GCAIDETGEPLPNKTVETCRKNEAVLLGAVGGPKWDKCKGDKRPESGLLKLRESLGLF
                     ANLRPATMYESIKEASPLRTDIVEKGIDFVVVRELTGGIYFGERGRKIIDGIENAYDV
                     EIYNENEIRRIGKRAFEIARNRNKKLISVDKANVLESSRLWRSIMEDLAKEFEDVELS
                     HMYVDNAAMQVVKDPSQFDVIVTNNIFGDIISDEASMITGSIGMLPSASLREDSFGMY
                     EPIHGSAPDIAGKDIVNPIATILSVSMMLRHSFNLEEEAKCIEDAVQSVLNKGYRTID
                     IYNGVGNVVGTREMGELIVNEI"
     gene            complement(1303..1794)
                     /gene="leuD"
                     /locus_tag="ACGI59_003422"
     CDS             complement(1303..1794)
                     /gene="leuD"
                     /locus_tag="ACGI59_003422"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HFG9741190.1"
                     /translation="MIANGSVFKFGDNIDTDVIIPARYLNIADYKELATHCMEDIDDK
                     FISKVKKGDIIVATKNFGCGSSREHAPIVIKESGVSCVIASTFARIFFRNSINIGLPI
                     LECEEAANNIDEGDNIEVDFSTGVIKNITKGKEYKAEPFPEFMQNIILNEGLINSIKA
                     NRG"
     gene            complement(1809..3086)
                     /gene="leuC"
                     /locus_tag="ACGI59_003423"
     CDS             complement(1809..3086)
                     /gene="leuC"
                     /locus_tag="ACGI59_003423"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HFG9741191.1"
                     /translation="MGMTMTQKILAKHAKLNEVKKGQLIEADLDVVLGNDITSPVAIR
                     EFEKLGIEDVYDKTKVVMVLDHFTPNKDIKSAEQCKFTRSFAKSKGVVNFFDVGDMGI
                     EHVLLPEKGIVTAGDVIIGADSHTCTYGALGAFSTGVGSTDMGAGMATGKCWFKVPGA
                     IKFVLKNKPNKWISGKDIILHIIGEIGVDGALYKSMEFCGDGVEYLSMDDRFTICNMA
                     IEAGAKNGIFPVDDKTMEYINSHKCSTMTKDVNIYEADEDAVYDEVYEIDLAELKETV
                     AFPHLPENTRTVDEIDKDIKIDQVVIGSCTNGRISDLEVVAEIMKGKKVADGVRVMIF
                     PGTQKVYLEAIEKGYITTFIEAGAAVSTPTCGPCLGGHMGILAAGEKSISTTNRNFVG
                     RMGHVDSEVYLASPAVAAASAITGKISKPSEII"
     gene            complement(3043..4785)
                     /gene="leuA"
                     /locus_tag="ACGI59_003424"
     CDS             complement(3043..4785)
                     /gene="leuA"
                     /locus_tag="ACGI59_003424"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HFG9741192.1"
                     /translation="MKCGKYKKYDKMQIVNRKWPDNEIFKAPIWCSVDLRDGNQSLPT
                     PMSVNEKVRMFKMLIDTGFKEIEVGFPSASNTEYTFLRKLIDENMIPDDVTIQVLTQS
                     RAHLIEKTFESIRGCKKAIIHLYNSTSVLQRDVVFNMSKQEIIDIAVEGAKLFNEEVK
                     KYPETEFTFEYSPESFTGTEMDYALEICEAVIDVWKPTTQKKVIINLPSTVEMATPNV
                     YADQIEWFCKNISCRDSIILSLHTHNDRGTCTAASELGLLAGADRLEGTLFGNGERTG
                     NMDIVNVGLNLYTQGIDPELDFSNIDKIIGIYEDCTKLMVHDRHPYAGNLVHCAFSGS
                     HQDAIRKGMIAMKNRDNDYWEVPYLPIDPHDIGREYKEIIRINSQSGKGGAVYIMETD
                     YGFMIPKNMHSDFGNVVKMESDRIGEELSSEAIFNLFKKEYIEVESPYKVKKYKIKSM
                     DELNYENDDSNDTNMIEMTARISYMGNEQRIVGIGNGPVDSFNNALKQCGMKDYKFRY
                     YWEHALEEGSHSRGVAYVGIEHNNEVYFGVSISENINTAAINALMNAINKSYIEEEIK
                     NGDDYDAENISQTC"
     misc_binding    complement(4909..5155)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(5303..5749)
                     /locus_tag="ACGI59_003425"
     CDS             complement(5303..5749)
                     /locus_tag="ACGI59_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9741193.1"
                     /translation="MSKNKFIYMNYYDNGYFKKGMTIYMVLALIILATIFIYSTTILY
                     ILCIITAVFLFKQGRDFSNKYEGKVYITLDNHSILINNQCIFSIKSKQSQKFNYKIIE
                     KIEVVKNILNIYTDESNYKIRLRALSLEDEKKLLNIIDEKMKKFKA"
     gene            complement(5770..6489)
                     /locus_tag="ACGI59_003426"
     CDS             complement(5770..6489)
                     /locus_tag="ACGI59_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HFG9741194.1"
                     /translation="MKKSIMDVHCHTLISGHAHSTFKENVEEASKKNIKYLGISDHGP
                     NMPGGPHPFYFYNLHLLPREVQGVKILRGIEGNIMDYNGNLDVQEDMLQHLDYIIASL
                     HRPCIASGTKEENTNAILKVMDKPKVKIIGHPDDSRYPLDYEPIVKKAKDKNILLEIN
                     NSSLSSNSHRTGTWENVSHMLTLCKTYGARVILGTDSHICYSIGEFENAEKVLEAVDF
                     PDELVINYHEDEIIEFFDINF"
     gene            complement(6607..7881)
                     /locus_tag="ACGI59_003427"
     CDS             complement(6607..7881)
                     /locus_tag="ACGI59_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HFG9741195.1"
                     /translation="MFIMENDLTMQELLDQQEQVFSKVKVGELTTGKITAVRNDEVQL
                     GLDYGFDGIIPISELNIEKNQYIEDIYHIGDEITAVITKVSQKDGTITLSKLQLDKRN
                     DFAELQKAYDEHRIITVNVEKNIDKGVFANYNTYTFFIPISQLDTKFITDTSKFVGLN
                     LEVYIKELDVRKNRLVASHRDVLQERINKEREERRAQIKAEKEAERARIKQEREEEKA
                     RIKAAKEDLFNSLEVGQKRDGKVTKIMPYGAFVDIGGIEGLAHINNLAWTRVESVEDV
                     VSEGQEVEVYVLDVNKETKKIALALKDINNDPWDLIAKEVQIDDVVNVKVLRIIEKGA
                     FVQIKEGVDAYLPISELSDTRVAKVTNVVNIGDEVKVKILDFKPKTKRMLVSIKEATR
                     EPEEDITEYLEVEESLGSIGELFKDKFKDLEV"
BASE COUNT         2431 a         1587 c          817 g         3187 t
ORIGIN      
        1 tccaaaaaat aactttatca aaatagaaac tcaaagtaga tataaacctc tttttcgtta
       61 ttaaaatagt aaaaatatat ttgacttaat ataaaaaaga gtgtctcatt aactaataaa
      121 gtttattttg agacacccct cataaaacaa attatactac aatagatttt tactaattct
      181 acaagttata tatttaaatt atattattca aattaaacta taattctaaa tttcatttac
      241 tattaattcg cccatttccc ttgttcctac aacatttccc acaccattat agatatcaat
      301 agttctataa cctttattga gtacactttg tacagcatct tcaatacact tagcttcttc
      361 ttctaagtta aaactatgtc ttaacatcat gcttacagat agaatagtcg caattggatt
      421 tacaatatct tttccagcta tatcaggtgc acttccatgt attggttcat acataccaaa
      481 actatcttct ctaagtgatg ctgatggtaa catacctata gaacccgtaa tcatagatgc
      541 ttcatcagat attatatctc caaatatatt attagtcact attacatcaa attggctagg
      601 gtcttttaca acttgcatag cagcattatc aacatacata tgtgacagtt ctacatcttc
      661 aaactcttta gctaagtctt ccattatact tctccaaagt cttgaactct caagcacatt
      721 tgccttatct acacttataa gttttttatt tctatttctg gctatttcaa aagctctctt
      781 accaattctt cttatttcat tttcgttata tatttctaca tcataagcat tttcgatacc
      841 atcaatgatt tttcttccac gttctccaaa ataaatgccc cctgttaatt cccttacaac
      901 aacaaaatca atgcctttct caactatatc cgttctaagt ggtgaagctt cttttatact
      961 ttcatacatt gtagctggtc ttaaatttgc aaataaaccc aatgactctc ttaattttaa
     1021 aagaccagac tctggtcttt tatctccctt acatttatcc cactttggtc ctccaacagc
     1081 accaagcaat acagcttcgt tttttcgaca agtttcaaca gttttattag gtagaggttc
     1141 tccagtttca tcaatcgcgc atcctccagc taatacatat tcacaatcga atctatgatt
     1201 aaatttacaa caaattttat ttagaacctt tataccttca tctataattt ccggtccaac
     1261 accatcacct tttatgactg ctatattaca attcatactt cactaccccc tatttgcttt
     1321 tattgagtta attaagcctt catttaatat aatattttgc ataaactcag gaaatggttc
     1381 tgctttatat tcttttcctt ttgttatatt ttttatcaca cctgtagaaa aatctacttc
     1441 tatattatcc ccttcatcta tgttattggc agcttcttca cattcaagta taggaagccc
     1501 tatatttata gagtttctaa aaaatattct agcaaaagta gaagcaatca cacaggatac
     1561 tccactttct tttattacaa ttggtgcatg ttctcttgat gaaccacaac caaagttttt
     1621 agttgcaact ataatatctc ctttttttac cttagaaata aatttatcat caatatcctc
     1681 catacaatgc gttgctagtt ctttataatc agctatattt aaatatctag ctggtattat
     1741 tacatctgta tctatattgt caccgaattt aaaaacactt ccattggcaa tcataaattt
     1801 tacctccctt aaattatttc tgatggtttt gatatttttc cagttatagc tgatgctgct
     1861 gctacagctg ggcttgcaag atacacctct gaatcaacat gccccattct tcctacgaaa
     1921 ttcctatttg ttgttgaaat ggatttttct cctgctgcaa gtatacccat atgtccccct
     1981 agacatggac cacaagttgg tgtagaaact gctgctcctg cctctataaa agtggtaata
     2041 tacccttttt ctatagcttc aagatatact ttttgagttc caggaaatat cataactcta
     2101 actccatctg ctactttttt gcctttcatt atctcagcta ctacttcaag gtctgatatt
     2161 cttccatttg tacaagagcc tattacaact tggtctatct ttatatcctt atctatctca
     2221 tctactgttc tagtattttc aggaagatga ggaaaggcta ctgtttcttt tagttcagct
     2281 aaatctattt cgtatacttc atcatagact gcatcttcat ctgcttcata tatatttaca
     2341 tcttttgtca tagttgaaca cttatgtgaa tttatatatt ccatagtctt atcgtctact
     2401 ggaaaaattc cattttttgc tcctgcttct attgccatat tacaaatagt aaatctatca
     2461 tccatagata agtattctac tccatctcca caaaattcca ttgacttgta aagtgctcca
     2521 tcaacaccta tctcccctat tatatgaagt attatatctt taccacttat ccatttattt
     2581 ggtttattct tcaaaacaaa ttttattgct ccaggaacct taaaccaaca ttttcctgta
     2641 gccatacctg ctcccatatc agttgaacct acaccagtag aaaatgctcc taatgctcca
     2701 tatgtgcaag tatgagagtc agcacctata attacatctc cagcagttac tatacctttt
     2761 tcaggaagaa gaacatgctc tattcccata tctcctacat caaaaaaatt tacaactcct
     2821 tttgattttg cgaaacttct tgtaaattta cattgttctg cactctttat atctttatta
     2881 ggtgtaaagt ggtctaaaac catcactact ttagtcttat cataaacatc ttcaattcct
     2941 aatttttcaa attctctaat ggctactggt gatgttatat cattacctaa aactacatct
     3001 aaatctgctt cgattaattg ccctttctta acttcattta atttagcatg tttggctaat
     3061 attttctgcg tcatagtcat ccccattttt tatttcctcc tcaatataac ttttatttat
     3121 tgcattcata agtgcattaa tagctgctgt atttatgttt tccgatatac ttacaccaaa
     3181 atagacttca ttattgtgct ctattcctac ataagctacc cctcttgaat gtgacccttc
     3241 ctctaatgca tgttcccagt aatatctaaa tttataatct ttcatgccac attgctttag
     3301 tgcattatta aatgaatcta caggtccatt tcctatacca acaatccttt gttcatttcc
     3361 catataactt attcttgcag tcatctcaat catgttagta tcattagaat catcattttc
     3421 atagtttaat tcatccattg attttatttt atacttctta actttataag ggctctcaac
     3481 ttcaatatat tctttcttaa acaaattaaa tattgcttca ctactaagtt cttccccaat
     3541 tctatcagat tccattttga ctacatttcc aaaatcagaa tgcatattct taggtatcat
     3601 aaatccataa tctgtttcca ttatataaac agctcctcct tttccagatt ggctatttat
     3661 ccttataatt tctttgtatt ctcttccaat atcatgaggg tctattggta aatatggaac
     3721 ttcccagtaa tcattatctc tgtttttcat tgctatcatg ccttttctaa tagcatcttg
     3781 atgtgaacct gaaaaagcac aatgtacaag attacctgca tatggatgtc tatcatgtac
     3841 cataagtttt gtacaatctt catatatacc tattatctta tctatattgc taaaatctag
     3901 ctctgggtct attccttgtg tataaagatt aagtcccaca tttactatat ccatattccc
     3961 agttctttca ccatttccaa atagtgttcc ttcaagtcta tcggcaccag ccaataatcc
     4021 aagttcactt gcagccgtac aagttcctct atcattgtga gtatgcaaac ttagtataat
     4081 agaatcccta caacttatat ttttacaaaa ccattctatt tggtctgcat atacgtttgg
     4141 tgttgccatt tcaactgttg atggaagatt tattattact tttttttgag tagttggttt
     4201 ccaaacatct atcactgcct cacatatttc aagtgcataa tccatctctg ttcctgtaaa
     4261 actctctggt gaatattcaa aagtaaattc tgtttctgga tattttttta cttcttcatt
     4321 aaataatttt gctccttcaa ctgctatatc tattatttct tgtttactca tattaaaaac
     4381 cacatctctt tgaagaacag atgtagagtt atataaatgt ataattgctt ttttacatcc
     4441 ccttattgat tcaaatgttt tttctataag atgagcccta gattgtgtaa gtacttgtat
     4501 agttacatca tctggaatca tattctcatc tattaatttt cttaaaaatg tatactctgt
     4561 atttgatgct gatggaaatc ctacctctat ttctttaaat cctgtatcaa taagcatttt
     4621 gaacattctg actttttcat ttacactcat aggtgttggc agtgactgat ttccgtctct
     4681 taaatcgaca ctacaccaaa ttggagcctt aaatatttca ttatctggcc atttacgatt
     4741 tactatttgc attttatcat attttttata ctttccacac ttcatgttat ttcccccttt
     4801 ttctgttttt tgtattcagt atacctcata tcgacgcaat cctgcacaat tcataaggat
     4861 aaatgtcaat tttatcatta gaataaaaaa aacctcatct ctaataaaat tagagacgag
     4921 gttttgctcg cggtaccact ctatttagct ttctaaaaag ctcccttagt aatagccaat
     4981 acaataatta taatgactat atttcctata acgtggaatt actccgatta ttctaagatt
     5041 taaatcttag aaaacagctc ctagacaagt tcagaactat caactactaa atcacaccat
     5101 cctttagctc tctgaaagtt tcattgttct tactacttct attcatcact tttttaatca
     5161 tttaaatttt ctaaatattt tgatttaata gttattatat acacattaaa atgtattgtc
     5221 aacacaaaaa ataattatct caatttttta tttttgataa gcaattctat aaaacatgct
     5281 aactattgaa aacagtatga tattatgcct taaacttttt catcttttca tctatgatat
     5341 tcaataattt tttttcatct tccaaactca atgcccttaa tctaatttta taattagatt
     5401 cgtcagtgta tatatttaat atatttttta ctacttctat tttttctatt atcttataat
     5461 taaacttttg actttgttta gattttatac tgaatataca ttgattattt attaatattg
     5521 aatgattatc gagagtaata tatacttttc cctcatattt atttgaaaaa tcacgacctt
     5581 gtttaaatag aaaaactgct gttattatac ataaaatata taatattgtc gttgaatata
     5641 taaaaattgt agctaaaatt ataagtgcta aaaccatata tatagtcatt ccttttttaa
     5701 aataaccatt gtcataataa ttcatataga taaacttatt tttactcaaa ttttttcact
     5761 tccaagattc taaaaattta tatcaaaaaa ttctataatt tcatcttcat gataatttat
     5821 gactaattca tctggaaaat ctactgcttc taaaactttt tctgcatttt caaattctcc
     5881 tatagaataa catatatgag agtcagtacc aagaattact cttgcaccat atgttttaca
     5941 caatgtcaac atatgactta cattttccca agtacccgtt ctatgagagt ttgaacttaa
     6001 tgatgaatta tttatttcta aaaggatatt tttatcttta gcttttttta ctataggttc
     6061 ataatctaaa ggatacctac tgtcatctgg atgtccaatt atttttacct taggtttatc
     6121 cataactttt aaaatagcat ttgtattttc ttcttttgta ccagaagcta tacaaggtct
     6181 atgcaaactt gcgattatat aatccaaatg ttgcaacata tcttcttgaa catctaaatt
     6241 accattataa tccattatat ttccttctat tcctctaaga atctttacgc cttgcacttc
     6301 acgtggaagc aaatgcaaat tataaaaata gaatggatgt ggtcctcctg gcatattagg
     6361 tccatggtct gatattccta agtattttat attcttctta ctcgcctctt cgacattttc
     6421 cttgaaagta ctatgtgcat gtccacttat tagagtgtga caatgtacat ccataataga
     6481 ctttttcatt aaatttcctc ctaatatttt acataaattc gtgaaaatag ctaaagggct
     6541 atttcaaaac taaagctgtc ataatagaca gtgtaacgtt ttaaaacagc cccattttca
     6601 attattttac acttctaaat ctttaaattt atctttaaat aattctccaa tacttcctaa
     6661 agattcctct acttctaggt attctgttat atcctcttct ggctctctag ttgcttcttt
     6721 tattgaaacc aacattctct tagtctttgg tttaaaatca agaatcttaa ctttaacctc
     6781 atctccaata ttaacaacat tagtaacttt agccactcta gtatcactca attcagatat
     6841 tggtaaatac gcatctacac cttctttaat ttgaacaaaa gccccttttt ctattattct
     6901 aagaacttta acattgacaa catcatcaat ttgtacttct tttgctatta aatcccaagg
     6961 gtcattattt atgtctttta atgccaaggc aattttttta gtctctttat ttacatccaa
     7021 cacataaact tctacttctt gtccctctga aacaacatct tcaacacttt caactcttgt
     7081 ccaagctaaa ttatttatat gtgctaatcc ttcaatccca ccaatatcca caaatgcacc
     7141 ataaggcatt atctttgtca ccttaccatc tcttttttgt ccaacttcaa gagaattaaa
     7201 taagtcttct ttagctgctt tgatacgagc tttttcttct tctctttctt gcttaattct
     7261 tgctctttca gcttcttttt ctgccttaat ttgagctctt ctttcttctc tctctttatt
     7321 tatacgctct tgtaatacat ctctatgaga tgctacaagt ctattttttc taacatccaa
     7381 ttcttttata taaacttcta agttaagacc aacaaattta gatgtatctg taataaattt
     7441 tgtatcaagt tgagaaattg gtataaaaaa tgtatatgta ttgtaattag caaatacacc
     7501 tttatctata ttcttttcaa catttacagt tattattcta tgttcatcat aagccttttg
     7561 taactcggca aaatcatttc ttttgtcaag ttgtaattta gataaagtta ttgtaccatc
     7621 tttttgagat acttttgtta ttacagcagt tatttcatca cctatgtggt atatatcttc
     7681 tatatattga tttttttcta tgttcaattc acttataggt attattccat caaacccata
     7741 gtctaatcct aattgtactt catcatttct cactgcagtt atttttccag ttgtcaattc
     7801 tccaactttc actttgctga atacttgttc ttgctggtca agtaattctt gcattgttaa
     7861 atcattttcc attataaaca tcctttcctc ttattgatta tcatatctgt aaatattata
     7921 ccattaatat tgcaaatact tacatttttc ttactataat ttttaaaata aattcaactt
     7981 catatattta tacattatag aaaatatctt attaaattaa aa
//
LOCUS       DAYAPA010000157         7982 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.162, whole genome shotgun sequence.
ACCESSION   DAYAPA010000157 DAYAPA010000000
VERSION     DAYAPA010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7982
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.162"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            27..101
                     /locus_tag="ACGI59_003428"
     tRNA            27..101
                     /locus_tag="ACGI59_003428"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:59..61,aa:Thr,seq:tgt)
     gene            116..200
                     /locus_tag="ACGI59_003429"
     tRNA            116..200
                     /locus_tag="ACGI59_003429"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:150..152,aa:Tyr,seq:gta)
     gene            210..293
                     /locus_tag="ACGI59_003430"
     tRNA            210..293
                     /locus_tag="ACGI59_003430"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:244..246,aa:Leu,seq:tag)
     gene            317..391
                     /locus_tag="ACGI59_003431"
     tRNA            317..391
                     /locus_tag="ACGI59_003431"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:349..351,aa:Gly,seq:gcc)
     gene            416..492
                     /locus_tag="ACGI59_003432"
     tRNA            416..492
                     /locus_tag="ACGI59_003432"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:450..452,aa:Arg,seq:tct)
     gene            502..577
                     /locus_tag="ACGI59_003433"
     tRNA            502..577
                     /locus_tag="ACGI59_003433"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:535..537,aa:Gln,seq:ttg)
     gene            586..661
                     /locus_tag="ACGI59_003434"
     tRNA            586..661
                     /locus_tag="ACGI59_003434"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:619..621,aa:Lys,seq:ttt)
     gene            664..752
                     /locus_tag="ACGI59_003435"
     tRNA            664..752
                     /locus_tag="ACGI59_003435"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:698..700,aa:Ser,seq:tga)
     gene            756..831
                     /locus_tag="ACGI59_003436"
     tRNA            756..831
                     /locus_tag="ACGI59_003436"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:789..791,aa:Phe,seq:gaa)
     gene            838..911
                     /locus_tag="ACGI59_003437"
     tRNA            838..911
                     /locus_tag="ACGI59_003437"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:872..874,aa:Met,seq:cat)
     gene            926..1002
                     /locus_tag="ACGI59_003438"
     tRNA            926..1002
                     /locus_tag="ACGI59_003438"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:960..962,aa:Ile,seq:cat)
     gene            1020..1096
                     /locus_tag="ACGI59_003439"
     tRNA            1020..1096
                     /locus_tag="ACGI59_003439"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1054..1056,aa:His,seq:gtg)
     gene            1105..1180
                     /locus_tag="ACGI59_003440"
     tRNA            1105..1180
                     /locus_tag="ACGI59_003440"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1138..1140,aa:Lys,seq:ttt)
     gene            1188..1261
                     /locus_tag="ACGI59_003441"
     tRNA            1188..1261
                     /locus_tag="ACGI59_003441"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1220..1222,aa:Cys,seq:gca)
     gene            1269..1345
                     /locus_tag="ACGI59_003442"
     tRNA            1269..1345
                     /locus_tag="ACGI59_003442"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1303..1305,aa:Arg,seq:acg)
     gene            1358..1433
                     /locus_tag="ACGI59_003443"
     tRNA            1358..1433
                     /locus_tag="ACGI59_003443"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1391..1393,aa:Val,seq:tac)
     gene            1509..1922
                     /locus_tag="ACGI59_003444"
     CDS             1509..1922
                     /locus_tag="ACGI59_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9741196.1"
                     /translation="MYIVIGNEIVDSEELKIIIDKNSEFKVEKDLSKSTKREDVIAYQ
                     LSIDLNYLDSLINEQCNLASLSDEEKFDEYMTLSDELALDLEELMPKYTIINARAYKL
                     DEVDGIVKIILAVAYADLGHLKLSDVVKRLSRQVD"
     gene            2231..5662
                     /locus_tag="ACGI59_003445"
     CDS             2231..5662
                     /locus_tag="ACGI59_003445"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HFG9741197.1"
                     /translation="MLKKFNKILVANRGEIAIRIFRACSELGIKSVGIYSKEDKYGLF
                     RTKADESYLIGEGKGPIDAYLDMDGIIDLAKRKKVDAIHPGYGFLAENAEFARKCEEN
                     GITFIGPSSKVMNMMGDKINSKKIAKEVNVQTIPGVEKAIRSTEEAKEVANKIGYPVM
                     IKASNGGGGRGMRIVHREEDLELEYETACSESRKAFGEDIIFIEKYIADPKHIEVQIL
                     GDNYGNIVHLYERDCSVQRRHQKIIEYAPAFSLDDKVRKEICEDAVKLSKHVGYSNAG
                     TLEFLVDANGGHYFIEMNTRVQVEHTVTEMVTGIDIVQSQILIAQGYSLDSEEINIKS
                     QDDVEIRGYSIQCRITTEDPKNKFMPDTGKIQVYRTGSGFGIRLDGGNGFTGANISPH
                     YDSLLVKTISWDRTFQGAINKTIRSIKELRVRGVKTNVGFLVNVLNNPIFSNGKCSTK
                     FIDENPDLFEITESKDRGTKLLQFIGDVIVNDNACKEKPLFDALHDPRMDKDGSKSEG
                     SKILFDKLGKSAYIEKIKNDKKLLLTDTTMRDAHQSLLATRIRTYDLLKAAKPTEKYQ
                     KDLFSLEMWGGATYDVAYRFLKESPWRRLQKLREEIPSIMFQMLLRASNGVGYKNYPD
                     NVIEEFTRESARQGIDVFRIFDSLNWVENMKPSINTALETGKIVEATMCYTGDILDKT
                     KTKYNLEYYIKMAQELESLGADIIAIKDMSGLLKPYSAYTLVKELKKNVKAPIHLHTH
                     DTSGNGVATCLMASEAGVDIIDAALESMAGLTSQPSLNAIVEALKNTERDTGIDLFGY
                     DELGKYYKDLRKVYNKFESDLTNSCAEIYNFEIPGGQYTNLKPQADSLGLVNRFDEVK
                     EKYKEANEVVGDIIKVTPSSKVVGDLAIFMTKNKLDKDNIIEEGKNLSFPDSVVDYCK
                     GMIGQPEGGIPKDLQEVVLKGEEAITVRPGSLLPAEDFDEIAKYLNEKYDINANIRNV
                     ISYALYPKVYEDYIKHLQHYNDISKLESDVFFYGLNKNEECEVEIEEGKVLTIRLVEI
                     GEVKENGFRTIGFELNGMVREVEIKDKNFSGKINNVEKADMNDPLQIGASIPGKVIKI
                     MVKEEDEVKANQPLIVIEAMKMETIIVAKTDGVIKSIKVKEDDMVEDKQLLMIMK"
     gene            5904..7832
                     /locus_tag="ACGI59_003446"
     CDS             5904..7832
                     /locus_tag="ACGI59_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFG9741198.1"
                     /translation="MKVYDSKMLRNVAVLGHSGCGKTNLIETIAYTANTNKIPKLTDK
                     VNMTYSMGLIPIEYNDYKFNLLDTPGYFDFSGDVVSSLRASDAAIIVIDATAPIQVGT
                     EKSLELTESIPKIMFINKIDNEKARYKDAIAMLREKYNNKIVPMISPIYKDKNFVKLH
                     NVFENIDDLEGEFKEQAMSVKEALMELIAETDDQILDKYFNGEELTTEEIQKGIIIGI
                     QRGDIIPVICGSTINNIGTKEILDTISSYLEPIFTEESKPFRGLVFKTMVDPFVGKMS
                     YIKITEGVLSKDKDVFNINKNVKEKIANIYTLRNSELVEIEKAKAGDIVVITKVNSLK
                     TGDTISADKDAEALEKIDFPKPQIYYAVTPKNKGDEEKVASVLNKLVEEDPTLHWYRN
                     TETKQALLGGQGELHIKTIKNKMKDKFGVDVELNDLKVPYRETIKGTADVQGKHKKQS
                     GGHGQYGDVKIRFERCESDFEFTEEIFGGSVPKQYIPAVEKGLKDSMQKGILAGYPVT
                     NIKATLYDGSYHDVDSSEMAFKMAASAAFKKGMEEAHPILLEPIMKLKITVPEEYMGD
                     VMGDINKRRGKIFGMEPDDKGKQIIFAEAPQAETFKYAIDLRAMTQGRGYFEMELERY
                     GEVPSQFAEKIIGLATAK"
BASE COUNT         3126 a         1017 c         1596 g         2243 t
ORIGIN      
        1 ccccttccag gtcgccaaaa taaaatgctg gtgtggctca acggtagagc agctgacttg
       61 taatcagcag gttgtaggtt cgattcctat caccagctcc atataaaatt aatacggagg
      121 atttcccgag cggccaaagg gggcagactg taaatctgtt gtcatcgact tcggtggttc
      181 gaatccacca tcctccacca aaaaaatgcg cggatgtggc ggaattggca gacgcactag
      241 acttaggatc tagcgccatt ggcgtggggg ttcgactccc ttcatccgca ccatttttat
      301 ttaatttaat aaacgagcgg gaatagttca gtggtagagc gcaaccttgc caaggttgaa
      361 gtcgcgagtt cgaatctcgt ttcccgctcc aaattttatg tttatgttgt aatgtgggtg
      421 catagctcag ctggatagag caacgccctt ctaaggcgtg tgtccggggt tcgaatccct
      481 gtgcgctcac cacatatata ttggggatta gccaagtcgg taaggcacat gactttgact
      541 catgtatgcg taggttcgag tcctgcatcc ccagccaaac agtatggtct attagctcag
      601 tcggtagagc acctgacttt taatcagggt gtcccgcgtt cgagtcgcgg atagatcacc
      661 aatggagagg tgtccgagtg gtttaaggag ctggtcttga aaaccagtga cttcgaaagg
      721 ggccgtgggt tcgaatccca ccctctccgc caaatgccca gatagctcag tcggtagagc
      781 aggggactga aaatccccgt gtcggtggtt cgattccgcc tctgggcacc aaaatgtggc
      841 ggtatagctt agttggctag agcgttcggt tcatacccga aaggtcatag gttcgactcc
      901 tattaccgct actgaaattt aatgtgggcc attagctcag ttggttagag cgcccggctc
      961 ataaccggta ggtctggggt tcgagtccct gatggcccac cacattaaat taacttatgg
     1021 tgggtatagc tcagttggtt agagcgccag attgtggctc tggaggtcgt gagttcgact
     1081 ctcattatcc accccaactt atatggtcta ttagctcagt cggtagagca cctgactttt
     1141 aatcagggtg tcccgcgttc gagtcgcgga tagatcacca aaaaggcggc gacatagcca
     1201 agtggtaagg cagtggactg caactccttg atccccagtt cgaatctggg tgtcgcctcc
     1261 aataaaatgc gctcatagct caactggata gagtgtctga ctacgaatca gaaggttagg
     1321 ggttcgagtc cctttgggcg caccatatga atagcgaggg attatagctc agctgggaga
     1381 gcacctgcct tacaagcagg gggtcacagg ttcgagccct gttaatccca ccagtgcaaa
     1441 cctctagtaa aactactaga ggttttattt ttgattgaaa aagtttttat atttaggagg
     1501 taaaaaattt gtatatagta ataggaaatg aaatagttga tagtgaagaa cttaaaataa
     1561 ttatagataa aaatagtgaa tttaaagttg aaaaagattt aagtaaaagc acaaaaagag
     1621 aagatgtaat agcatatcag ttaagtatag atttaaatta cctagattct cttataaatg
     1681 agcaatgtaa tttagccagt ctatctgatg aagagaaatt tgatgaatat atgactttgt
     1741 ctgatgaact tgcactggat ttagaagagt taatgccaaa atatacaata ataaatgcta
     1801 gagcatataa attagacgag gtagatggaa ttgtaaaaat aatactagct gtagcgtatg
     1861 cagatttagg gcacttgaaa ctttctgatg tagtaaagag gctatctaga caagtagatt
     1921 aggatatgaa tatgaactat attaaaaaaa tgtataaaat cttacgtaaa gatgaaaata
     1981 attatgtaaa catagaaaaa aacagtataa aatcaagtca attgtagaaa ttttcacata
     2041 aattcatctg aaattctata aaaaatgcct taattttaat gaggtaaatc gagtttacac
     2101 agtctaaaaa ttacttatat ttataaattt atcttattaa ttaaggaact ttcttaaaca
     2161 tcttcaaaaa taaagtattt tagtctataa tctttaatat tacacaaaca ataaaggaga
     2221 agaatagagt atgcttaaaa agtttaataa aatactagtg gctaatagag gcgaaatagc
     2281 tataagaata tttagagcct gttcagaatt ggggataaaa agtgttggta tatacagtaa
     2341 ggaagataag tatggattgt ttagaacaaa agcggatgaa tcatatttga taggagaagg
     2401 taaagggcca atagatgcat atttagatat ggatggaata atagacctag caaagagaaa
     2461 aaaagttgat gctatacatc caggatatgg ttttctagcg gagaatgcag aatttgctcg
     2521 taaatgtgaa gagaatggaa ttacttttat aggaccatca tctaaggtaa tgaatatgat
     2581 gggggataag attaactcta aaaaaatcgc aaaagaagtt aatgttcaaa ctataccagg
     2641 agtagaaaaa gcaataagaa gtactgaaga agcaaaggaa gttgcaaaca aaataggata
     2701 tcctgttatg ataaaagcat ctaatggcgg tggcggtaga ggtatgagaa ttgtacatcg
     2761 cgaagaagac ttagagcttg aatatgaaac tgcttgtagt gaatcaagaa aagcttttgg
     2821 agaagatatt atatttatag aaaaatatat agctgatcca aaacatatag aagttcaaat
     2881 attaggtgat aattatggaa atatagttca cttatatgaa agagattgtt cagttcaaag
     2941 aagacatcaa aaaataatag aatatgcacc agctttttca ttagatgata aagtaagaaa
     3001 agagatatgt gaagatgcag ttaagttatc aaaacatgtg ggttattcta atgcgggaac
     3061 attagaattt ttagtagatg caaatggtgg gcattatttt atagagatga acacaagagt
     3121 acaggttgaa catactgtaa cagagatggt tacaggaata gatatagttc aaagtcaaat
     3181 actaatagcc caaggttaca gcttagatag tgaagaaatt aatataaaat cacaagatga
     3241 tgtggaaata agaggttact ctatccaatg tagaataact acagaagacc ctaagaataa
     3301 attcatgcct gatacaggta aaattcaagt ttatagaact ggttcaggat ttggtataag
     3361 actagatgga ggaaatggat ttacaggggc aaacataagt cctcattatg atagtttatt
     3421 ggttaagaca atatcttggg atagaacatt ccaaggagca ataaataaaa ctataagatc
     3481 tataaaagaa cttagagtta gaggtgttaa aactaatgta ggattcctag ttaatgtttt
     3541 aaataatcct atattctcaa atggtaaatg tagtactaag tttatagatg aaaatccaga
     3601 tttatttgaa ataacagaaa gtaaagacag aggaacaaaa ttacttcaat ttataggaga
     3661 tgtaattgta aatgataatg cttgtaaaga aaaaccatta tttgatgcat tacatgaccc
     3721 aagaatggat aaggatggtt caaaatcaga aggaagtaaa atacttttcg ataagttagg
     3781 taaatctgca tatatagaaa agataaaaaa tgataagaaa ttactattaa cagatacaac
     3841 aatgagagat gcacatcaat cattattagc aacaagaata agaacatatg acttattaaa
     3901 agctgctaag ccaacagaaa aatatcaaaa ggatttattc tcacttgaaa tgtggggagg
     3961 agctacttat gatgttgcat atagattttt aaaagaatct ccttggcgta gattacaaaa
     4021 gctaagagaa gaaataccaa gcataatgtt ccaaatgttg cttagagcat caaatggagt
     4081 tggatataaa aactatccag ataatgtaat agaagaattt acaagagaat ctgctagaca
     4141 aggtatagat gtatttagaa tatttgattc attaaactgg gttgaaaata tgaaaccatc
     4201 tattaataca gctttagaaa ctggaaaaat tgttgaagca actatgtgtt atacaggtga
     4261 tatactagat aagactaaaa ctaaatacaa tctagaatat tatattaaaa tggcacaaga
     4321 attagaaagc ttgggagcag atataatagc aataaaagat atgtcaggtc ttcttaaacc
     4381 atattcagct tacacattag ttaaagagtt aaagaaaaat gtaaaagcac caatacattt
     4441 acacactcat gatacaagtg gaaatggtgt agcaacttgt ttaatggcgt cagaagctgg
     4501 agtagacata attgatgctg cattagaatc tatggcagga cttacaagtc agccatcact
     4561 taatgctata gtagaggcac ttaagaatac agagagagat acaggaatag atttatttgg
     4621 atatgatgag ttaggaaaat attataagga tttaagaaaa gtttataata aatttgaaag
     4681 tgatttaact aattcttgtg cagaaatata taattttgaa ataccaggag gccaatatac
     4741 aaacttaaaa cctcaagctg atagtttagg attagttaat agatttgatg aagttaaaga
     4801 aaaatataaa gaagctaatg aagttgtagg agatataata aaagtaactc catcttcaaa
     4861 agttgtagga gacttagcta tctttatgac taaaaataaa ttagataaag acaatataat
     4921 agaagaaggt aaaaatttat cattcccaga ttctgttgtg gattactgta aaggtatgat
     4981 tggtcaacca gaaggtggaa taccaaaaga tttacaagag gtagttctaa aaggtgaaga
     5041 agctataaca gtaagaccag gttcattatt accagcagaa gattttgatg aaatagctaa
     5101 atatctaaat gaaaagtatg atataaatgc taatataaga aatgttataa gttatgcttt
     5161 atatccaaaa gtatatgaag attatatcaa acatctacaa cattataatg atatatctaa
     5221 acttgaaagt gatgtattct tctatggatt aaataaaaat gaagaatgtg aagtagaaat
     5281 agaagaaggt aaggtattaa caataagatt agttgaaatt ggtgaagtta aggaaaatgg
     5341 atttagaact ataggatttg agttaaatgg aatggttaga gaagtagaaa taaaagataa
     5401 gaatttctct ggaaaaatta ataatgttga aaaagctgat atgaatgacc ctcttcaaat
     5461 tggagcaagc ataccaggaa aagttataaa aataatggtt aaagaagaag atgaagtaaa
     5521 agcaaatcaa ccattaatcg ttatagaggc tatgaagatg gaaactataa tagttgctaa
     5581 gactgatgga gtaattaagt caatcaaagt aaaagaagat gatatggttg aagataaaca
     5641 attattaatg ataatgaagt aatttaatta aaataaatta aaaagcactc tgaaatgttt
     5701 aataaaaaat gttttagagt gtttttattt attttaatta aatatttgtt aaaaaaaaga
     5761 ggacaattac ctccagatgt agaaataaag ctagtaagga aaatgttatc taaaaattct
     5821 aaatatagta aatattttcc ataatatttc ttaggaaata acagcaaaga taaaatacat
     5881 aaatttaaaa ggtgggtgct tatatgaagg tttatgatag taaaatgttg agaaatgtag
     5941 cggtattagg acatagcgga tgtggaaaaa caaatctaat cgaaactata gcttacacag
     6001 caaacactaa taagatacct aaattaacag acaaggtaaa tatgacttat agcatgggat
     6061 taatacctat agagtataat gattataagt ttaacttatt ggatacccca ggatattttg
     6121 actttagcgg agacgttgtg tcatctctta gagcaagtga tgcagcaata atagtaatag
     6181 atgcaacagc gcctattcaa gttggtacag agaaatcatt agaactaact gagagtattc
     6241 ctaagataat gtttataaat aaaatagata atgaaaaggc aagatacaaa gatgcaatag
     6301 caatgttaag agaaaaatac aataataaga tagttcctat gataagtcct atatacaaag
     6361 ataaaaactt tgtaaagtta cataatgttt ttgaaaacat agatgattta gaaggtgaat
     6421 ttaaggaaca agctatgagt gttaaagaag cattaatgga acttatagca gaaactgatg
     6481 accaaatatt agataaatat tttaatggag aagaactaac tacagaagag atacaaaaag
     6541 gtattattat aggtatacaa agaggtgata ttatacctgt aatatgtggt tcaactataa
     6601 ataatatagg cacaaaggaa atattagata caatatcaag ttatttagaa cctatcttta
     6661 cagaggaaag caaaccattt aggggattag tatttaaaac tatggttgac ccatttgttg
     6721 gaaagatgtc atatataaaa attactgagg gtgtactttc taaggataaa gatgttttta
     6781 atataaataa aaatgtaaaa gaaaaaatag ctaatatata tacattgaga aatagtgaat
     6841 tagtagaaat agaaaaagct aaagctggtg atatagtagt tataactaaa gttaattctc
     6901 taaaaacagg agacactata tctgcagata aagatgcaga agctttagaa aagatagatt
     6961 ttcctaaacc acaaatatat tatgcagtaa ctcctaagaa taaaggtgat gaagaaaaag
     7021 tagcatctgt attaaataaa ctagtagaag aggaccctac attacattgg tatagaaata
     7081 cagagacaaa acaagcactt ttaggaggtc aaggagaact tcatataaaa acaattaaaa
     7141 ataaaatgaa agataagttt ggtgttgatg ttgaattgaa tgaccttaaa gttccttata
     7201 gagaaactat aaaaggaaca gctgatgtac aaggaaaaca taaaaagcaa tcaggtggtc
     7261 atggtcaata tggagacgta aaaataagat ttgaaagatg tgaatctgat tttgaattta
     7321 cagaagaaat atttggtggt tctgtaccaa aacaatatat accagcagtt gaaaaaggtt
     7381 taaaagactc tatgcaaaaa ggtatattag caggatatcc tgttacaaac ataaaagcta
     7441 cgctttatga tggttcatac catgatgtag actcttctga aatggccttt aaaatggcag
     7501 caagtgcagc ctttaaaaaa ggtatggaag aagcacaccc aatactacta gaacctataa
     7561 tgaaacttaa gataactgtt ccagaagaat atatgggaga tgtcatgggt gatataaata
     7621 agagaagagg aaaaatattt ggaatggaac cagatgacaa agggaaacaa ataatatttg
     7681 cagaagctcc acaagcagag acatttaaat atgcaataga tttaagagca atgacacaag
     7741 gtagaggata ctttgaaatg gaattagaga gatatggaga ggttccatca cagtttgcag
     7801 aaaaaataat tgggttagct actgcaaagt aatgaacaaa ataaatagaa aaataattaa
     7861 tatatattat taaaaatatt tttagggttt taactaggta taggtttatg ttaggtaaac
     7921 ttatacctag tttttataca tttttgatga ataaaaaaat atataatact ttcttaaata
     7981 tt
//
LOCUS       DAYAPA010000158         7981 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.163, whole genome shotgun sequence.
ACCESSION   DAYAPA010000158 DAYAPA010000000
VERSION     DAYAPA010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7981
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.163"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(295..1050)
                     /gene="dapB"
                     /locus_tag="ACGI59_003447"
     CDS             complement(295..1050)
                     /gene="dapB"
                     /locus_tag="ACGI59_003447"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFG9741199.1"
                     /translation="MLKVIISGYNGTMGRVLAECVKKDNELELVCGVARSADEKGHHS
                     DTKLYKTMTDVTEKTDVIIDFSHHETLEPILSYALKNNTPVVLATTGYDENDLNKIEE
                     ASKNIPILLSSNTSLGVNILIKLVKDASKMLEGFDIEIIEKHHNRKEDAPSGTATMIA
                     NAIREVLPESENTHGRYGRDCKRKPNEIGIHSIRGGNIISDHDVVFAGDNEVLTLSHH
                     SQSDEVFANGSIVAAKFLIGKQSGLYKMSDILG"
     gene            1490..2479
                     /locus_tag="ACGI59_003448"
     CDS             1490..2479
                     /locus_tag="ACGI59_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFG9741200.1"
                     /translation="MLKEEKTYQEKDAKVFNGVAALIMILLFLVVSIGIMVFGIVKLD
                     SGFTGIGSLMLILGLVFIVVDFILFFGLKMINPKEAIVLVLFGNYYGTIKKEGYYWVN
                     PFCSAINPAVSRINVSKSSSDEKVDISTNSRGKKVSLKTMTLNNEKQKVNDLLGNPII
                     IGVVVIWKVIDATKAVFNVDNYNTFLSIQCDSTIRNVSRLYPYDVSEDGDEKSLRGSS
                     QEIADRLKDELQSRVDIAGIEVCEVRITHLSYAPEIAAAMLQRQQAEAIIAARKKIVE
                     GAVSMVEMALTNLSENNVVTLDEERKAAMVSNLLVVLCGNKDAQPVVNSSSIY"
     gene            complement(2835..3551)
                     /gene="dapD"
                     /locus_tag="ACGI59_003449"
     CDS             complement(2835..3551)
                     /gene="dapD"
                     /locus_tag="ACGI59_003449"
                     /EC_number="2.3.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047200 - tetrahydrodipicolinate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HFG9741201.1"
                     /translation="MVNLNDAYEIARYIKEVKKKTPVKVYVNTNSLHFQSTDKFKVFG
                     SNGSYIFVGDYEDIMEVLDKHGNDITDTHVEYDRRNSAIPLKNTLSEHARIEPGAIIR
                     DMVTIEKNAVVMMGAVINIGAVIGEGSMVDMNAVIGARGTLGKNVHLGAGAVVAGVLE
                     PPSATPVIVEDDVLIGANAVILEGVRIGKGAVVAAGAVVTTDVEAGAVVAGSPAKVIK
                     MKDEKTADKTKLMEDLRNLD"
     gene            complement(3775..4524)
                     /gene="dapB"
                     /locus_tag="ACGI59_003450"
     CDS             complement(3775..4524)
                     /gene="dapB"
                     /locus_tag="ACGI59_003450"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFG9741202.1"
                     /translation="MLKVIATGYDGKMGKILADTIREDNELELVCVAARGLDSYEGDL
                     KIYEDMSTIVEEADVVIDFSHHSNLDNILSYVLKTKTPLVIATTGYNDDEMNKIREAA
                     KVIPVFQSYNMSLGVNVLVKLVKDAAKLLEGFDIEIIEKHHNRKVDAPSGTAVMIANA
                     IKEVLPNVENNYGRYGRSAKRQPNEVGIHAIRGGNIVGEHDALFAGDNEVLTISHQAQ
                     SRGIFASGSIAAAKYLVKQKPGFYNMDSMLG"
     gene            complement(4591..5478)
                     /gene="dapA"
                     /locus_tag="ACGI59_003451"
     CDS             complement(4591..5478)
                     /gene="dapA"
                     /locus_tag="ACGI59_003451"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFG9741203.1"
                     /translation="MIFKGSAVALVTPFTKDNKVDFDKLGELVEYQIANGTDAIVSCG
                     TTGEANTMTDEEQLATIKYVVEKVNKRVPVIAGSGSNDTMHSVNLSQEAEKLGVDALL
                     IITPYYNKANKAGLKRHFETIANSVKLPIILYNVPGRTCVNISPSLIVELAKIDNIVA
                     VKEASGDLGQVAEIASLVPDDFAIYSGNDDTILPLLSLGGQGVISVLANVCPQETHDL
                     VAKFFEGDIEGSRKLQLGMDALIAALFIEVNPIPVKTAMNLLGFNVGDLRLPLAEMDP
                     ANLEVLKKELVNFGLKVNA"
     gene            complement(5595..6596)
                     /locus_tag="ACGI59_003452"
     CDS             complement(5595..6596)
                     /locus_tag="ACGI59_003452"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFG9741204.1"
                     /translation="MKKVNVAIVGATGMVGRTFLKVLEERNFPIENLFLFSSSKSAGS
                     KVMFCGKEYIVEELKETSFTDRGIQIALFSAGGSISEKYAPIAASNGILVVDNSSQWR
                     MNPDVPLVVPEVNPEAIKNHKGIIANPNCSTIQAMVPLKALDDKYKIKRVVYSTYQAV
                     SGSGVKGVEDLEKGMNGNPTGFYPHQISYNCLPHIDSFTENGYTKEEMKMVNETMKIL
                     DNYDLKITATTVRVPVKNGHSESINVEFNNPFELDDLVKTLEEAPGVVVVDNPAKNEY
                     PTAVEFDDRDEVFVGRIRRDFSVDNGVNLWVVADNIRKGAATNAIQIAEMALSYDLV"
     regulatory      complement(6769..6943)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            complement(7021..7902)
                     /gene="dapA"
                     /locus_tag="ACGI59_003453"
     CDS             complement(7021..7902)
                     /gene="dapA"
                     /locus_tag="ACGI59_003453"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFG9741205.1"
                     /translation="MLFKGSAVALVTPFTEDNNVNFEKLGELIEYHIENGTDALVVCG
                     TTGEATTMSESEIFAVIKYTVEKVNKRIPVIAGTGSNNTMLSVHMSQEAEKLGVDGLL
                     IITPYYNKTNEKGLKLHFETIANSVKLPIILYNVPGRTKVNIKPSVVAELAKIDNIVA
                     VKEASGDLAQVAEIAKLVPKDFAIYSGNDDTILPLLSLGGSGVISVLANICPKETHDL
                     VTKFFEGDIEGSKKLQLDMDALIAALFIEVNPVPVKTAMNILGFNVGDLRLPLAEMEE
                     TNLNVLKQELTNFGFKF"
BASE COUNT         2738 a         1290 c         1035 g         2918 t
ORIGIN      
        1 ggataatatt taataggttt atatgatgaa aagattataa aataataaac aatatgattc
       61 catataccaa taaataggat aatatttaat aggtttatat gatgaagaga ttataaaata
      121 ataaacaata taattccata taccaataaa taagataatc tttaagaaaa atgattatat
      181 ttaaaaggta aacataaaat ttataaactt ttagaataga aatctcaatc aattagatat
      241 tcaaattgat gaatatgaat acctaaatag attgagattt attatgtaca aaacttatcc
      301 taatatatct gacattttat aaagcccaga ttgcttacca attaaaaatt tagctgcaac
      361 tatagaaccg tttgcaaata cttcatcaga ttgagaatga tggcttaatg taagaacttc
      421 attatcacct gcaaatacaa catcatggtc gctgattata ttgccacctc ttatagagtg
      481 tattccaatt tcatttggtt ttcttttaca atctctacca tatctaccat gtgtattttc
      541 actttcaggc aagacttctc taattgcatt agctatcata gtagcagttc cactaggggc
      601 gtcttctttt ctattgtgat gtttttctat gatttcaata tcaaaacctt ctaacatctt
      661 tgaagcatcc ttaactaatt ttattaatat attcactcca agagatgtat ttgatgataa
      721 taatatagga atgttttttg atgcttcttc tattttattt aagtcatttt catcatatcc
      781 agtagtagct aatactacag gtgtattatt ttttagagca tatgacaata ttggctctaa
      841 agtctcatga tgtgaaaagt ctattattac atcagttttt tcagttacat cagtcattgt
      901 tttgtaaagc tttgtatcac tatgatgacc tttttcatca gcagacctag ctacaccaca
      961 aaccaactct aattcattat ctttttttac acattctgct aaaactcttc ccattgttcc
     1021 attatagcca ctaattataa ctttcaacat aaattcctcc tatgaaatta ataaaaattt
     1081 gttatatttc tataagttta ttttatcact aatataattt atataaaata atattagtga
     1141 taataagctt acaatttatc ttacattaaa aaatttaagt ggatttttca aaatatagat
     1201 aaatttcttt ttcattgtat aaatgataaa aatatatttg acttcatacc aaaaagagtg
     1261 ccccatgaac taaaaagtta attttgaaat accatattca tatatcatat gatttatttg
     1321 gaaatcaata ttaatacatt aaaataacta aatacatgta tgcataaaga tgtataaaaa
     1381 atatataaac atatagtatt aattttaaat tcaacaattt aattgttaaa agtgttatta
     1441 ttaaattaga aatatcttaa cttaatatac tgagatggga gtgatagata tgttaaaaga
     1501 agaaaaaact tatcaagaga aagacgcaaa agtattcaat ggagtggcag ctttgataat
     1561 gattttatta tttttagtag tttcaatagg tatcatggtt tttgggatag taaaacttga
     1621 tagtggattt actggaatag gttcacttat gttaattttg ggacttgtat ttatagtagt
     1681 agattttatc ctattttttg ggctaaaaat gattaatcct aaagaagcta tagttctagt
     1741 tttgtttggt aactattatg gaactataaa aaaagaaggg tactattggg tgaatccatt
     1801 ttgttcagca ataaacccag cagtatcaag aattaatgta tcgaaatctt catctgatga
     1861 aaaggtagat atatcaacta attcaagggg caaaaaagta tctttaaaaa caatgactct
     1921 taataatgaa aagcagaaag tcaatgattt attgggaaat ccaattataa taggagtggt
     1981 tgtaatttgg aaggttatag atgcaacaaa ggcagtattt aatgtagata attataatac
     2041 attcttatct attcagtgtg attctactat tcgcaatgtt tcaagacttt atccatatga
     2101 tgtatctgaa gatggagatg aaaaatctct tcgtggtagt agtcaggaaa tagcagatag
     2161 attaaaagat gaattacaat caagggttga tattgcagga attgaagtgt gtgaagtgag
     2221 aattactcat ctttcttatg caccagagat tgcagcagca atgttacaac gtcaacaagc
     2281 agaggctata atagcagcac gtaaaaagat tgttgagggg gctgtaagta tggtagagat
     2341 ggcattgact aatttaagtg aaaataatgt tgtgactttg gacgaagaac gtaaagcagc
     2401 tatggtaagt aatctgttag ttgtgctttg tggcaataaa gatgcacaac cagtggtaaa
     2461 cagcagttct atttattaaa ttataaaaag tttaatagtt tattttattg atatatagaa
     2521 aatttagtag ttcattttat taaattatat aaagattaat ggttttcact ttattaaatt
     2581 ataaaaagtt taatagttta ttacattgat atattgaaaa attgaaggaa ataaaaatac
     2641 ttgtcagcaa gaaataaaaa tgatataaga atgaataaga gaattgtaaa aaaataaaat
     2701 gattataaat aaaagataaa aatattgtaa aaaagagggt gcctctggtg aattaaattc
     2761 acacagagac acctttttaa ttaatttttt aaattagtat attattgttt aattaatagt
     2821 atagtactat ttaattaatc taaatttcta aggtcttcca taagcttagt tttgtcagcc
     2881 gttttttcat ctttcatttt tataacttta gcaggagaac cagcaacaac ggcaccagct
     2941 tctacatcag tagttacaac agcaccagct gcaacaacag cacctttacc gattctaaca
     3001 ccttcaagaa taactgcatt agctcctatt aaaacatcat cttcaactat aacaggagta
     3061 gcagaaggtg gctctaaaac accagcaaca acagcaccag cacctaagtg aacattttta
     3121 ccaagagttc ctctagctcc aataacagca ttcatatcta ccatagaacc ttcaccaata
     3181 acagctccta tatttataac agctcccatc attacaacag catttttttc tatagtaacc
     3241 atgtctctta ttattgcacc tggctcaatt cttgcatgtt cacttaaagt attttttaga
     3301 ggaatagcag aatttcttct atcatattct acatgagtat ctgttatatc atttccatgt
     3361 ttatctaata cttccattat atcctcatag tcacctacaa atatataaga accattactt
     3421 ccgaatacct taaatttatc agttgattgg aagtgtaaag agttagtatt tacatatacc
     3481 ttaacaggtg ttttcttttt aacttctttt atataccttg ctatttcata agcatcgttt
     3541 aaatttacca tttgttaatt ctccttgtat ttcattatat ttcttttatt atatacttat
     3601 ttgtcaattt tagtatatac ttacttctta ttatttatat tttattttag ttagttttta
     3661 ttgtatagac atagtattaa ataaatttat tatatatgtt tgatttgtat tgcgtattat
     3721 tatatctata ttgcatatgt ttaatttata ttgtgtatta ttatatttat gctattatcc
     3781 caacatgctg tccatattat aaaatcctgg tttttgctta actaagtatt tggcagcagc
     3841 tatagaacca cttgcaaaga taccacgaga ttgagcttga tggcttattg taagaacctc
     3901 attgtctcca gcaaatagag catcatgttc gccaacaata tttccacctc ttatagcatg
     3961 aataccaact tcatttggtt gtctttttgc actacgacca tatctaccat aattattttc
     4021 aacgtttggc aaaacctctt ttatagcgtt agcaatcata acagctgtgc cacttggggc
     4081 atctactttt ctgttgtgat gtttttctat tatttctata tcaaatcctt ctagtaattt
     4141 agctgcatct ttaaccaatt ttactaatac atttacacca agagacatat tatatgattg
     4201 gaatacagga ataactttag ccgcttcacg tattttgttc atttcgtcat cattatatcc
     4261 agtagtagca attactaaag gtgttttagt ctttaaaacg taagataaga tattatctaa
     4321 attagagtga tgagaaaaat ctataactac atcagcttcc tcaactatag tactcatatc
     4381 ttcataaatt ttaaggtcgc cttcatagct atccagacct ctagctgcaa cacaaaccaa
     4441 ttctaattca ttatcttctc ttatagtatc ggctaaaatc ttacccattt ttccatcata
     4501 accagttgct ataactttta acataatttc ctcctatgat taagggctaa cttttgacca
     4561 aacttacttt ttaccatgca ttaatcttta ctatgcatta acttttagcc caaagttaac
     4621 taattctttc tttaatactt ctaaatttgc tgggtccatt tctgctagag gtaatcttaa
     4681 atctccaaca ttgaatccca ataaattcat tgcagtctta actggtatag gatttacttc
     4741 tataaataaa gctgcgatta gagcatccat accaagttgt aattttctag aaccttcaat
     4801 atcgccttca aagaatttag ctaccaaatc gtgagtttct tgagggcaaa cattagcaag
     4861 tactgatata actccttgac ctcctaaaga taataaagga agtatagtat catcattacc
     4921 agagtagata gcaaagtcat ctggaactaa actagctatt tctgcaacct gtcctaggtc
     4981 tccactagct tcttttacag caactatgtt atcaatttta gcaagttcaa ctataaggct
     5041 aggacttata tttacacaag ttcttccagg gacattgtaa agtataattg gaagtttaac
     5101 actgttagct attgtttcaa aatgacgctt aagtccagct ttattagctt tattgtaata
     5161 aggtgttatt attaatagag catctactcc taatttttca gcttcttgac ttaagttaac
     5221 tgagtgcata gtatcattag aaccacttcc agctataaca ggaactcttt tattaacttt
     5281 ttcaacaaca tacttaatag tagcaagttg ttcttcatca gtcatagtat tagcttcacc
     5341 agtagtacca caagatacta tagcatcagt accattagct atttggtatt caactaattc
     5401 acctaatttg tcaaaatcaa ctttgttatc ctttgtaaat ggtgtaacta gagcaacagc
     5461 agaaccttta aatatcatat aaaatcaatc ccctcttttt tatttttgtg ttttatatta
     5521 cagttacttc taatgcttgt ttttgagtta ctattaatat tttacaaaat tatatattta
     5581 tacaagtttg tatattatac taaatcgtat gataaagcca tttcagctat ttgaattgcg
     5641 ttagttgcag caccttttct tatattatca gcaacaaccc aaagatttac accattatca
     5701 acactaaaat ctcttcttat tcttccaaca aacacttcat ctctatcatc aaattctaca
     5761 gcagttggat attcgttttt agcaggatta tcaactacta caacacctgg agcttcttca
     5821 agtgttttta ctaaatcatc taactcaaat ggattgttaa actcaacatt tatagattca
     5881 ctatgaccat tttttactgg aactcttaca gtagttgcag ttatttttaa atcgtagtta
     5941 tcaagtattt tcatagtttc atttaccatt ttcatttctt ctttagtgta tccattttca
     6001 gtgaatgaat ctatatgagg aagacagttg taagagattt gatgagggta gaatccagta
     6061 ggattaccgt tcattccttt ctctaaatct tcaacacctt taacaccaga accagataca
     6121 gcttggtaag tagagtaaac aactctctta attttgtatt tatcatcaag agctttaagg
     6181 ggaaccatag cttgtatagt agagcagtta ggattagcta ttataccctt atgatttttt
     6241 atggcttctg gattaacctc aggaactact aaaggcacat ctggattcat tctccattga
     6301 ctagagttat ctactactaa gattccattt gaagcagcta ttggagcata cttttcacta
     6361 atacttccac cagcagagaa taatgctatt tgtatgcctc tatcagtaaa tgaagtttcc
     6421 tttaattctt ctactatata ttctttacca caaaacatta ctttagaacc agctgattta
     6481 gaagaagaga ataaaaatag attttctatt ggaaaatttc tttcttctaa tacttttaaa
     6541 aatgttctac ctaccattcc agtagcacca actattgcta catttacttt tttcatataa
     6601 attcacccca attttaaatt attaaaacat tattttattt tagttataca agtaagatat
     6661 gttaatattt ttatatatcc aaaaacttta atattatatt aacaacttta attatataca
     6721 aaaaacgtac aaaggtatac tttgtacgtc taatttatca ctcaaagtaa accctgtagc
     6781 tcatcaccta caaaaataag tgacagtatt acacatgtta tgtgcaatcc cagaaaatat
     6841 tattgcaaga aatattttct tcggctgtaa atcctttttc caatcattaa cacctgccag
     6901 ctactattgg atactcttag ttacagctac ctctacccta ggttaatagg atattaaaat
     6961 ttgattattc taataataat acatatttaa gaaaaaatca acttatttat ataaatattg
     7021 ctaaaattta aatccaaaat ttgtcaattc ttgttttaac acattaagat tagtttcttc
     7081 catttcagct agaggaagtc ttaaatcacc aacattaaaa cctaaaatgt tcatggcagt
     7141 tttaacagga actggattta cttctataaa taaagcagca attaaagcat ccatatcaag
     7201 ttgtaatttt tttgagcctt caatatcacc ttcaaagaat ttagttacta aatcatgtgt
     7261 ttctttagga catatgttag caagtacaga gataacacca cttccaccta atgataatag
     7321 tggtagtata gtatcatcat taccggagta tatagcaaag tctttaggta ctaatttagc
     7381 tatttctgca acttgagcta agtctccact tgcttccttc acagcaacta tattatctat
     7441 tttagcaagt tcagccacaa cagatggctt tatattcact ttagttcttc ctggtacgtt
     7501 gtataaaata attggcaact taacgctatt tgctattgtt tcaaagtgaa gtttaagacc
     7561 tttctcatta gtcttattat agtaaggagt tattattaaa agaccatcca cccctaattt
     7621 ttcagcttct tgactcatat gaacagacaa catagtattg tttgaaccag tacctgctat
     7681 aacaggtatt ctcttattaa ctttttcaac tgtatatttt attacagcaa aaatttcaga
     7741 ttcactcata gtagtcgcct cacctgttgt tccacaaaca actagtgcat ctgtgccatt
     7801 ctcaatgtga tattcgatta attcacctaa tttttcaaag ttgacattat tatcctctgt
     7861 aaatggggta actaaagcaa cagcagaacc tttaaataac atataatcaa atctcctttt
     7921 ttatctaaat ttaatttttt aagtacaata ataattatag tacaaattaa ttaattttaa
     7981 t
//
LOCUS       DAYAPA010000159         7830 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.164, whole genome shotgun sequence.
ACCESSION   DAYAPA010000159 DAYAPA010000000
VERSION     DAYAPA010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7830
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.164"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(202..345)
                     /locus_tag="ACGI59_003454"
     CDS             complement(202..345)
                     /locus_tag="ACGI59_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9741206.1"
                     /translation="MSEFLLGVLASLTASFITYIISRKVKSHSGRSDFELDVKIKFNK
                     KRH"
     gene            604..792
                     /locus_tag="ACGI59_003455"
     CDS             604..792
                     /locus_tag="ACGI59_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9741207.1"
                     /translation="MTKTILCDYCNKGINKDDNKYITFHKKSHMKTNICINCALNLID
                     KDKLNENIINNQHDYSKK"
     gene            1494..2015
                     /locus_tag="ACGI59_003456"
     CDS             1494..2015
                     /locus_tag="ACGI59_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFG9741208.1"
                     /translation="MDFKIRELINDITQDIIQTYKIQIPIVNINQVVDALGGKVIEDS
                     SLSGYSDGFIRKVDDSFEIVVSPYQPDTRKNFTIAHELGHLFLHMGYGIDDELWNSQD
                     GNQYFRSGNTNKEYQSNEFAAALLMPKHEYKRIMDENTVGNKVDTSKIAEYFNVSSYA
                     ASNRGKWLGYLQW"
     gene            2009..3577
                     /locus_tag="ACGI59_003457"
     CDS             2009..3577
                     /locus_tag="ACGI59_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9741209.1"
                     /translation="MVDDKEYKTQNVNNVHNSAKNENAFNLKKYKEKLQENINTDIYE
                     KEKEPNHPEVILFFSFDIANSSLYKNINYSGWAKVLSHIIRKLQYRVYENLKAQLWRV
                     LGDEVIFIIVLKNYDEIYKYIDIIFDILTSTAKDIKSGNIFSTLEGFSESEKYLMKLQ
                     NIISLKGAAWIAIVSRNPNFNALENNEQYENISAMYDLSNNYKIFEFLGNDIDAGFRI
                     SKQTCPERLVLSFELAYILSRKTDILSKLHIITYKKLKGIWKDKLYPIIWYHNKGKNN
                     DIEFDDSFSFDEIEENELVREYFFNKKGESKLLIDSFMFNSVDKALDKILIDRNLSDK
                     IEKIGDVISKTNPSYDKNTIDKDYIKVDLMELHCVAVCYNKSTSKILIAKRSDNRNNN
                     ASKWEFGCAKASLETSIINTIKDEYEKDFNINIEPITDCTRKDDCQPIPLAIYQVKKS
                     DGLHKGIITLAEIINDYDISKFEPTSKHNELAWIGEDELEDFNENTVPDFKETLKLAF
                     KKLNENQLQESTNM"
     gene            complement(3641..4159)
                     /locus_tag="ACGI59_003458"
     CDS             complement(3641..4159)
                     /locus_tag="ACGI59_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Pycsar system effector family protein"
                     /protein_id="HFG9741210.1"
                     /translation="MDKKIDIAKFIVNRIDYYIEKSDNKASFLLLLNSAIIGFLFSGK
                     EKIMNHLSISNIRCLEMLFYIVILCIFVISIYFSIMVLKPRNSKIENEYKSIFYYKEI
                     ASLNNEQYKEAFENAFKDEENLINDALVQIKELSLICDKKMYNVKKSVEAFILGGIIL
                     FIYILVVVFNAI"
     gene            complement(4325..4672)
                     /locus_tag="ACGI59_003459"
     CDS             complement(4325..4672)
                     /locus_tag="ACGI59_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9741211.1"
                     /translation="MDALVYERFVRAAFSIKLNNLINRSEDLGGLAEADIFRAANNLH
                     ELNEIKIGTGYAIAIFNNEILESCNVSDNDSNRMIELFDRSLIATSREEILDIIREYE
                     TYRGRYLTFNWKR"
     gene            complement(4768..5580)
                     /locus_tag="ACGI59_003460"
     CDS             complement(4768..5580)
                     /locus_tag="ACGI59_003460"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFG9741212.1"
                     /translation="MKICITVGHSILKSGACTSADGVINEYQYNKSLAPVLADTFRKE
                     GHKVDVIICPEKQFKTKNEEKSYKIPRVNSGGYDLLIELHLNASNGQGKGSEVLYYSN
                     KGLEYATRICDKLGTVFKNRGAKLDKRLYILNSSKPTAVLIESFFCDNKEDYDKAKKL
                     GHEGIAKLIVEGVLNKNINNEGVKQMYKHTIVYDGEVDKILANVLSWGYSPSKVLVCD
                     IKDYVPGQTENLYVVGGGACEKISSITKEKFIMIKGNDRFDTLYKALDFINR"
     gene            complement(5580..6011)
                     /locus_tag="ACGI59_003461"
     CDS             complement(5580..6011)
                     /locus_tag="ACGI59_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFG9741213.1"
                     /translation="MNLTIVFLATNIFIKLVILAIAFDTLLGCLRAIKTHQFNSSFGI
                     NGGIRKVAMIACIFFLAVVDILTKFNFLFMLPQDWIDFLRLNHLGISEFFSILFILYE
                     SVSILKNMYLCGLPVPKKLKEKIGNLLDTMTDELNIKGGNK"
     gene            complement(6079..6297)
                     /locus_tag="ACGI59_003462"
     CDS             complement(6079..6297)
                     /locus_tag="ACGI59_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9741214.1"
                     /translation="MKKIIVICILVLMLIIPTMGEIFIMVENIKLEGLEQNPTSCYIS
                     NPGIMGEAKCDAAYFIIAADFVNNLKVK"
     gene            complement(6395..6577)
                     /locus_tag="ACGI59_003463"
     CDS             complement(6395..6577)
                     /locus_tag="ACGI59_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015981517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9741215.1"
                     /translation="MNINNVVVRILAERILNGGLNPLKNREFELDDVTNTEYRKAVED
                     YIIRESGVVEGAEPTK"
     gene            complement(6577..6870)
                     /locus_tag="ACGI59_003464"
     CDS             complement(6577..6870)
                     /locus_tag="ACGI59_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9741216.1"
                     /translation="MNVPNRVIYDQTGKIIFETGESCGDVLPHNKITELDYIDIPYGS
                     IDYIKNRIIGINIETKQPILEEIPVYVSEEKKRIQELENQVLLNENEKVGGLL"
     gene            complement(6886..>7830)
                     /locus_tag="ACGI59_003465"
     CDS             complement(6886..>7830)
                     /locus_tag="ACGI59_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9741217.1"
                     /translation="LITLLQALPFLTVSSYNSTQATFKADHTDPTLLTKIEVFIDDVL
                     KETITTDLTTEKTINYTLEDNALHTLKIVVTDSNNATAEKVLSISKNIMPLPENVNLN
                     DISTKLVEVNAGFKVGKTSIINTLALKNIEASLNNTLVELSEKIKQSFDSGDASLQDL
                     MNQLTQANNTISQLNSKYKVASGTVTSFADSAKIAYPYLTDRTFKPGTWVKISNLDFK
                     PNIFFADFDYYDTEYKNNYKLFLFACRGVATQRGVDFSSVTAFIRKNSDENFHANGWL
                     YNNSEGDVYFNNTGVQIPAYNFDSTQRHIYKWYAIKFI"
BASE COUNT         2849 a         1110 c          909 g         2962 t
ORIGIN      
        1 agatagtgtt ctttttgttt ttatgaaagt tttttagggg gataataata tatatactta
       61 gagataaaat aaaatatatt tctagcattc acagttttat tttttcttaa aatgcgatat
      121 aataaagatg taaattcgta tcaaaaacaa aaaaagaaac tacaatctat tagcctagag
      181 tgaagtttca ttaactaaat attaatgtcg ttttttatta aacttgattt ttacatcaag
      241 ctcaaagtca ctcctgccag agtggctttt tacttttctg gaaataatat atgtaataaa
      301 gctagctgtt aaactagcta acactcctag taaaaattcg ctcatttctc cacctccttc
      361 cttttaaggg atggaatgta gaaaatgaag ctccactctt agattgtagt tccacaagat
      421 tattcttgca ttttaattat aacataattt tacaattatc aaatatccat tctccatatt
      481 tttttattta tacaatatat tctatattgc aataaaaaac attgttttaa atatatatac
      541 ataggtatat actaaatatg ttaaacttaa ttatggaaaa tataaaatga aaggaataaa
      601 aatatgacta agactatatt atgtgattac tgtaataaag gaataaataa agatgataat
      661 aagtatatta cttttcataa gaaaagtcat atgaaaacta acatttgtat taattgtgca
      721 ttaaatttga tagataaaga taaattaaat gaaaatatta taaataatca acatgattat
      781 tcaaagaaat gaaaaagcac tctccataat gaagaatgct ctatataata atgtttgact
      841 tagtaaagat gtgttggggt tacatattta cttttttatt atatcatata actttgtgta
      901 tgaaaaagaa tttaaatcaa ttttaaggtg tgttgagtaa tgttcttgat agtttatatg
      961 ttgatgaatt tcaaaaaaat aagcactctt ataaaaagag tacttttggt atatattcaa
     1021 gcatttatct aatacaatta tagcatgtat tatgttttag tatgataatt ttcgttcgtt
     1081 ttattattac aacttctact atagttttca tactttaaca tcaactaaat gaatttaatt
     1141 aagattacta gttaatcgtt ttttggttct ttcatattct gaaatctaaa attttattat
     1201 ttgtttattg ttattattta acacatgttg gtatttcaac gatttatctt attgttaata
     1261 ttcttattgc ttccaaagta tatctaatta tttttaagat aatttactaa tttttatttt
     1321 ttcaaacata cattcgacaa aaaacagttt ttatatggta taattatatt gtataataca
     1381 aaaggttaat gaaaataatt aatattaaat gtaccaaaaa aaatagtttt tgatataatg
     1441 aatattataa taaataatta tttaaataaa atattatata aggatgtgat tttatggatt
     1501 tcaaaatcag agagctaatt aatgatataa cccaagatat tatccaaaca tacaaaatcc
     1561 aaattccaat agtaaatata aatcaagttg ttgatgcttt aggaggcaag gtaatagaag
     1621 atagttcttt aagtggatac tctgatggat ttattagaaa agttgatgat tcatttgaaa
     1681 tagtggtatc tccttatcaa ccagatacca gaaagaattt taccattgct catgaacttg
     1741 gacatttatt tttacacatg ggttatggca ttgatgatga actatggaat agtcaagatg
     1801 gaaatcagta ttttagaagt ggcaatacca ataaggagta tcaatccaat gaatttgcag
     1861 cagccttgtt gatgcctaaa catgaatata aaagaattat ggatgaaaac acagtaggta
     1921 ataaggtcga cacttcaaaa attgcagaat actttaatgt ttcctcttat gcagcatcta
     1981 atagagggaa atggttagga tatttacaat ggtagatgat aaagaatata aaactcaaaa
     2041 tgtaaataat gttcataatt cagctaaaaa cgaaaacgct tttaatctta aaaagtataa
     2101 ggaaaaactt caagaaaata ttaatactga tatttatgaa aaagaaaaag aacctaatca
     2161 tccagaagtg attttgttct tttcttttga tatagctaat tcatcattat ataaaaatat
     2221 aaattatagc ggatgggcta aggtattatc acatataatt cgtaagttac aatatagagt
     2281 ttatgaaaac cttaaagcac aactttggag agttcttggt gatgaggtaa ttttcattat
     2341 cgtactaaaa aattatgatg aaatttataa atacatagat ataatctttg atattttaac
     2401 aagcactgct aaagatataa aaagtggtaa tatattctct acactagaag gattttctga
     2461 atctgaaaaa tacttaatga aacttcaaaa tattatttca ttaaaaggag cggcctggat
     2521 tgctatagta tcaagaaatc ctaattttaa tgctttagaa aataatgaac aatatgaaaa
     2581 tatttctgct atgtacgatt tatccaataa ttataaaata tttgagttct taggaaatga
     2641 tattgatgct ggtttcagaa tatcaaagca aacatgccca gaaagacttg ttcttagttt
     2701 tgaactagct tatatattat caagaaaaac tgatatttta tctaaattac atataattac
     2761 atataaaaaa ttaaagggta tctggaaaga taaactatac cctatcattt ggtatcataa
     2821 taaaggaaaa aataatgata tagaatttga tgatagtttt tcttttgatg aaatagaaga
     2881 aaatgaattg gttcgagaat atttttttaa taaaaaagga gaaagtaaat tactaattga
     2941 ttcttttatg tttaattctg tagacaaggc tttggataaa atacttatag acagaaacct
     3001 tagtgataag attgaaaaaa taggtgacgt aatttctaaa acaaacccca gttatgataa
     3061 aaatacaata gataaagact atataaaagt agatttaatg gaattacact gtgttgcggt
     3121 ttgttataat aaatcaactt caaaaatatt aattgcgaaa agaagtgata atagaaataa
     3181 taatgcgagt aaatgggaat ttggttgtgc taaggcaagt ctagaaactt caattataaa
     3241 tactattaaa gatgaatatg aaaaagattt taatataaac attgaaccta ttactgattg
     3301 cacaagaaaa gatgattgcc aacctatacc tctagcaatt tatcaagtta aaaaaagtga
     3361 tggtttacac aaaggcatta taactctcgc agaaataata aatgattatg atatttctaa
     3421 atttgaaccc acatcaaaac ataatgaact tgcatggata ggagaggatg aacttgaaga
     3481 ttttaatgaa aacacagtac ctgattttaa agaaacttta aaactagcat ttaaaaaatt
     3541 aaatgaaaat caattacaag aatctacaaa tatgtaaata gtattcttct atgatttgat
     3601 tctatcttca tagaggaata ctaaccaaat aaaacatttt ctaaatagca ttaaaaacta
     3661 ctacaagtat atatataaac aatataatac cacctaaaat aaaagcttct actgactttt
     3721 ttacattata cattttctta tcacagatta aagataattc ttttatttga acaagtgcat
     3781 cattaatcaa gttttcttcg tctttaaatg cattttcaaa tgcttctttg tactgttcat
     3841 tatttaaaga tgctatctct ttataataaa aaatagattt atattcattt tctattttag
     3901 aattccttgg ctttaaaacc ataatcgaaa aataaattga aataacaaat atacataata
     3961 taacaatgta aaatagcatc tctaaacatc tgatattact tatacttaag tgattcatta
     4021 ttttttcctt ccctgaaaat aaaaaaccta taattgcgct atttaataat agtaaaaaac
     4081 tagctttatt atctgatttt tcaatatagt aatcaattct atttactata aacttcgcta
     4141 tgtctatttt tttatccatg attttactac ctcatttcat taaattttat attgattcaa
     4201 aattttattt tctacttatt atcattgaat aaattattat ttggaaataa tcttattgaa
     4261 ataataaaaa tatgtgaaag aaagtaataa ctaactataa ttactacttc ctaacttaaa
     4321 cattctatct cttccaatta aaagtaagat atcgtcctct ataagtttca tattctctta
     4381 taatatccaa tatttcctcc ctagaagttg caattaaact tctatcaaat agttcaatca
     4441 ttctattgct atcattatca gaaacattac aagactctaa tatctcatta ttaaatattg
     4501 caatagcata cccagtacct atttttatct catttaattc atgtaaatta tttgctgctc
     4561 taaagatatc agcttcagcc aatccaccta aatcttcgct tctattaatc aagttattaa
     4621 gtttaatact aaaagctgct cttacaaatc tctcataaac caatgcatcc atacataaat
     4681 cctccattat cacaataata tatttacatt atattacaat aatagagtta tcacaacaaa
     4741 caaccacttc tagttgataa cttttatcta tctattaata aaatccaatg ctttataaag
     4801 tgtatcaaat ctatcattac cttttatcat aataaatttt tctttagtaa tagaacttat
     4861 cttttcacat gcgccacctc ctacaacata taaattttcc gtctgacctg gtacgtaatc
     4921 ttttatatca caaactaaaa ctttgcttgg actatagccc caactaagca cattcgcaag
     4981 tatcttatca acttctccat cataaacaat tgtatgtttg tacatctgtt taactccctc
     5041 attatttata tttttattta atacaccttc tacaattaac ttagcaatac cttcatgacc
     5101 tagtttctta gctttatcat aatcttcttt attatcacag aagaaacttt caattaatac
     5161 tgctgtaggc tttgaactat ttaagatata taatctttta tctaatttag cacctctatt
     5221 tttaaatact gtacctagtt tatcacatat tctagttgca tactctaagc ctttattact
     5281 ataatatagg acttctgaac ctttaccttg accgttactt gcatttaaat gtaactctat
     5341 aagtaaatca tatcctccac tattaactct aggtatttta taagactttt cttcattctt
     5401 agttttaaac tgcttttctg ggcatattat tacatctacc ttatgccctt cttttctaaa
     5461 tgtatctgct aatactggtg caagagattt gttgtattga tactcgttaa ttactccatc
     5521 agcagaagtg catgctccac tttttaaaat actgtgtcct actgtaatac atattttcat
     5581 tatttattac ctcccttaat atttaattcg tctgtcattg tatctagtaa attgcctatt
     5641 ttctccttta attttttggg aactggtaat ccacataagt acatattttt taatatactt
     5701 acactttcat acaaaatgaa tagaatagag aaaaattcag atattccaag atgatttaat
     5761 ctcaagaaat caatccaatc ttgtggcaac ataaataaaa agttaaactt tgtaagtata
     5821 tcaactactg ctagaaaaaa tatacatgct atcattgcaa cttttcttat tcctccattt
     5881 attccaaaac ttgaattaaa ctgatgtgtt tttattgctc ttaagcaacc taacagtgta
     5941 tcaaatgcta ttgctaatat tactaatttt ataaatatat ttgttgctaa aaaaactatt
     6001 gttaaattca tatttcctcc taatttttgt attaaaaagg acctagaatt atctaagtcc
     6061 tttaaatgta ttaatttatt attttacctt taaattgtta acgaaatctg cagctataat
     6121 aaagtatgct gcatcacatt ttgcttctcc cataatccct ggattagaaa tgtaacaact
     6181 tgttgggttt tgttctagcc cctctagctt tatattttct accattataa atatctcccc
     6241 catagtaggt ataattaaca ttagaactag aatgcaaatt acaattattt tcttcatttt
     6301 ttaatctcct tttaatagaa tttttttatt atagataaaa ataaaaaata tataactaaa
     6361 aaaggaaatt atttttaatt tatataaaaa aaacctattt tgttggttct gctccttcta
     6421 ctactccact ttctctaatt atataatcct ctactgcttt tctatactct gtgttagtta
     6481 cgtcatctaa ttcaaattct cgatttttta gagggtttaa tcctccgttt aatatcctct
     6541 ctgctaatat tcttactaca acattattta tattcattat aataatcctc ctactttttc
     6601 attttcattt aataaaactt gattttctaa ttcttgtatt ctcttttttt cttcacttac
     6661 atatactggt atttcttcca aaattggttg ttttgtttct atatttatgc ctataattct
     6721 atttttaata taatctatac ttccatatgg aatatcaata taatctaatt cagttatttt
     6781 attgtgtggt aacacatccc cacaggactc acctgtttca aaaattattt tgcctgtttg
     6841 gtcatatatt actctgtttg gaacattcat atttttttct ccttcttaaa taaatttaat
     6901 agcataccat ttataaatat gtctttgagt tgagtcaaag ttataagctg gaatttgaac
     6961 accagtatta ttaaaataaa catccccttc ggaattatta tagagccaac cattagcatg
     7021 aaaattttca tcactgtttt ttctaataaa agctgtaacg cttgaaaaat caacacctct
     7081 ttgtgtagcg acaccacgac aagcaaaaag gaaaagttta taattatttt tatattcagt
     7141 atcatagtaa tcaaaatcag caaagaaaat gttaggctta aaatctaaat tactaatttt
     7201 aacccaagta ccaggtttaa aagttctgtc ggttagatat ggataagcaa ttttagcact
     7261 atccgcaaaa gaagtgactg ttccactagc aactttatat ttagagttta attgcgatat
     7321 agtattatta gcttgtgtta actgattcat caaatcctgt aaactagcgt ctccactatc
     7381 aaaagactgc tttattttct ctgataactc aacaagtgtg ttattcaaac ttgcttctat
     7441 attctttaat gctaaagtgt ttataatact tgttttccca actttaaatc ctgcattaac
     7501 ctcaactaat tttgttgata tatcatttaa atttacattt tcgggcagtg gcattatatt
     7561 cttacttata cttaaaactt tttcagcagt tgcattattg ctgtctgtaa caactatttt
     7621 aagcgtgtgc agtgcattat cttctaatgt atagttaatt gttttctctg ttgttaaatc
     7681 tgttgttata gtttctttta gtacatcatc tataaatact tctatcttag ttaatagtgt
     7741 agggtctgtg tggtcagctt taaatgttgc ttgtgtggag ttataagagg atacggttaa
     7801 aaaaggtaat gcttgtagta atgttattag
//
LOCUS       DAYAPA010000160         7660 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.165, whole genome shotgun sequence.
ACCESSION   DAYAPA010000160 DAYAPA010000000
VERSION     DAYAPA010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7660
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.165"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            169..1443
                     /locus_tag="ACGI59_003466"
     CDS             169..1443
                     /locus_tag="ACGI59_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster-binding protein"
                     /protein_id="HFG9741218.1"
                     /translation="MITISHITAKNMYKSLEERINKFPQGAPPSDTLYKILNVLYTEQ
                     EAKLVAQLPIKPFRVKTAAKIWSVSESEAYRVLDKLASKALILDIEDNKGKKYIMPPP
                     MAGFFEFAMMRTRHDIDQKLLAELYYQYMNVEEDFIKDLFYSTETKLGRVYVQEEVLT
                     NDNEVSILDYERATHIIDESTHIGISMCYCRHRMQHVGKACDAPMDICMTFDNVANSL
                     INNKFARRVDKIECKELLHQAYEHNLVQCGENVRKGVTFICNCCGCCCEAMVAAKRFG
                     NLHPVQTTSFIPNINHENCVKCGKCITACPIDAISKVKEDGKEYIKIDEDRCLGCGVC
                     VRNCHKNSIMLLKRDEKIITPANSVHRAVLMAIEKGQLQNLIFDNNALASHRAMGAIL
                     SAILKLEPAKKILASKQLKSVYLDKLLSMNDK"
     gene            complement(1604..4645)
                     /locus_tag="ACGI59_003467"
     CDS             complement(1604..4645)
                     /locus_tag="ACGI59_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp20"
                     /protein_id="HFG9741219.1"
                     /translation="MFKQRLSKLLSSTLVLSMLFTAAPNITFADNTKDNSEKYQSSDI
                     ELHDYSKNAESYTKTKALAKEKIQTLLSKYGAVSAQYALIDNGKIEISGNGGVYSKQD
                     NKNLNKDNMYSIASISKMFTTTAVMKLVDDGKLNLDTPVVKYIPEFKMADDRYKEITP
                     RMLLNHSSGLMGSSFKNTILLADNDSYGHDNFLKELQKQRLKAKPGAFSVYCNDGFTL
                     AEILVERVSGMSFTNFLDKYINNPLNLQNTKTTENSFDSSKLAKAYVPYWEDAVPQDN
                     LNAIGAGGLYSSAENLCTFAQTFMKNSNGILSPASVKAMENKEYLNGLWPEGEDSILG
                     YGLGWDCVNTYPFNQYNLKALTKGGDSLLFHSNLIVLPDENMAVAVLSSGGSSQLNEI
                     IGQEILLSALKEKGKIKEIKPDKTFSKPQQVKMPSSLKENSGLYASSNMIKVDVNDNG
                     TLTVSSPYIENGPEDKYVYIGQDRFVSEKGNSCLKFVKEKNNITYLNMSSYDDVPGLG
                     QTASLYYVAQKIDDNNISNSVKEAWKKRNGKDYYLVDEKYTSQSYMFGSVKATLALSD
                     ETPGYIVNTKIMDENNSNAFIEIPGVIGRDLSDIKLHKENGTEYLSFGTLTYVSEDSI
                     TNLPAEKSFTCELESNGYAKWYKIGDDIANKKIEVNLPQNSAFAVYDDKGVPVNYSLV
                     TKNNRVRLPKGGVIVFLGSPNARFEVTYQDEVNASALTGTDRYETSIKISQAGWENAE
                     NAVLINDSAIADALAATPFAYKKNAPILLTGSSQINEKTLAELKRLKVKNVYVVGGEA
                     SINEKSLDTIKSNNISVSRISGSDRYQTSMNIAKELNNISNISKISVVNGEKGLADAV
                     SIGAVSAQNDMPIILTNENSNITEINNLFKNKKIDKSYVIGGEYTVSKNIESKLQNPQ
                     RISGSTRNETNAKVIKEFYKDSKIDNLYVAKNGMNKQDDLIDGLSVGVLAGKTKSPVM
                     LVGNSLDYNQKELFKTMRFKSVTQIGGNGNENSFKQIKEIA"
     gene            4851..5150
                     /locus_tag="ACGI59_003468"
     CDS             4851..5150
                     /locus_tag="ACGI59_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFG9741220.1"
                     /translation="MYKNINKKQLKEMMKNEKDVLLLDVRTKDEFKEYKIENAINISL
                     QELINNIDEIYDYKDKKVVVYCRSGHRSVTACNLLAEEGFEHLYNLNSGIIDYMS"
     gene            complement(5389..7617)
                     /locus_tag="ACGI59_003469"
     CDS             complement(5389..7617)
                     /locus_tag="ACGI59_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HFG9741221.1"
                     /translation="MRHLKKTNSLKEILNWNNIKNLRPSKGLKNILEIFKSDLKDIRG
                     NYAVLVIVLGLAFLPSLYAWFNIKASWDPYGSTANMLVAVTNEDKGNEIAGQKINVGD
                     QLIEQLKDNKSLGWQFVDEKTALEGVKKGTYYASLKIPKNFTKDLTSLITDDVKKGEI
                     IYTVNEKINAVAPKITDKGASTIQLQVNQTIVKTVSEIVLGISNGVGAGIEHSLPKLT
                     KVENSLIDLQKRFDHINKTVDLASDATSKLSDTVKDIKSDLPTIKKTLNDTKLLSSDL
                     KKFLEDTNDNLDELSPLIKSDLNLMVDLSSSASSLTLNLIDAVNSGSEDVPKLIDNLS
                     EKLSNLQSLNDTLVDFLTKLNQLTSNNRLDDVIDNLEDSSNKIDSSISTLNDIKNKVI
                     SGQQPSISALNNVLSLSNGIGRINLNILNNFDSKISKPINSIFANSIKVANDIITVLD
                     KAEAKLPKVEEILTTSLKLSGNAQESISLIRERLPLAKGMLDDLIDTLSKISNGEDMK
                     KLVSLLESDILTKSNFLKEPVEVKTEKLYPIENYGSAMTPFYSVLSTWVGILLISALL
                     STSVHGNYKPYEIYFGRGLTFLSIALVQGFIIGTGDILLLGVTAKHPILLIVLLMFTS
                     MVFNFIVYSLVSVFGNVGKAIAIVLLVVQIGSSGGTFPIQVTPTFFQRINPIMPFTYA
                     IGATREAVGGIYAPNLTKDILVLLLFMILSICLNVLLKGPINRVIKPFNDKFGDSNLT
                     GH"
BASE COUNT         2655 a         1097 c         1079 g         2829 t
ORIGIN      
        1 tataaaatat tattatatca cttaaataat tttttattta tataagaaat ctaataataa
       61 attactagag gtatttaact atttagaaag aattggtatt atataaagta agagaatata
      121 taataagaaa gaatatttga ttttattgga aaacaaaaga aaaagaaggt gattactatt
      181 tctcatataa ctgcgaagaa tatgtataaa agtttagagg aaagaataaa caagtttcca
      241 caaggtgctc caccatctga tacattatac aaaatactaa atgtactata tacagaacaa
      301 gaggcgaagt tagttgctca gcttccaata aagcctttca gagtaaaaac agcagctaaa
      361 atttggagtg taagtgaaag tgaagcatat agagtattgg ataaacttgc tagtaaggct
      421 ctgatactag atatagaaga taataaaggg aagaaatata taatgcctcc tccaatggct
      481 ggtttttttg agtttgccat gatgagaacg agacacgata tagaccaaaa acttttggca
      541 gagctttact atcaatatat gaatgtagaa gaagatttta tcaaagattt attttattca
      601 acagaaacga agcttggaag agtatacgtt caagaggaag ttttaactaa tgataatgag
      661 gttagtattt tagattatga aagggctact catataattg atgaatcaac tcatataggt
      721 ataagtatgt gttactgtag acatagaatg caacatgttg gaaaagcttg tgatgctcct
      781 atggatatat gtatgacgtt tgacaatgta gcaaattctt taataaataa taaatttgca
      841 agacgtgtgg ataaaataga atgtaaagag ctacttcatc aagcatatga acataactta
      901 gttcaatgtg gagaaaatgt taggaaaggg gttacgttta tatgtaattg ctgtggatgt
      961 tgctgtgaag caatggtagc agctaaaaga tttggaaatc ttcatccagt acagacaaca
     1021 agttttatac caaatataaa ccatgaaaat tgtgtaaagt gtggtaaatg tataactgca
     1081 tgtccaatag atgcgataag caaggttaag gaagatggta aagaatatat aaaaattgat
     1141 gaggatagat gtttaggttg tggagtttgt gttagaaatt gccataaaaa tagcattatg
     1201 ttattaaaga gagatgaaaa aataataact ccagcaaatt cagtacatag agcagttctt
     1261 atggcaattg aaaaagggca gttacaaaat ttaatttttg ataataatgc tctggctagt
     1321 catagagcta tgggagctat actgtcagct atattaaagc ttgaaccagc taaaaagatt
     1381 ttagccagta aacaattaaa gtcagtatat ttagataaat tgttaagtat gaatgataaa
     1441 tagaaactaa cttaaaaaag gcaaagtttt agacttatct atatcttaat aaacttatca
     1501 tttaatattt aaatttaatt tttacacata taaaacttat aacttaaata aaaaaaggct
     1561 atattcaatt aatattactt gaatacagcc ttttttagtt atgttaagct atttctttta
     1621 tttgcttaaa tgagttttca tttccatttc caccaatttg tgttacagat ttaaatctca
     1681 tagttttaaa tagttctttt tgattataat ctagtgagtt tcctactagc attacaggag
     1741 atttagtttt tccagccaaa actccaacag ataatccgtc tattaaatca tcttgtttat
     1801 tcattccatt tttagcaaca tataaattgt ctatcttaga atccttgtaa aattctttta
     1861 ttacttttgc attagtttca tttctagtgc tacctgatat tctctgtgga ttttgaagtt
     1921 tactttctat atttttagaa actgtatatt ctccaccgat tacataagat ttatcaatct
     1981 ttttgttttt aaacaagtta tttatttcag ttatattact attttcatta gtaagtatta
     2041 taggcatatc attttgagct gatacagctc caatgcttac tgcatcggct aatccttttt
     2101 cgccattaac tactgaaatc tttgatatat tgctaatatt gttcaattct ttagcaatgt
     2161 tcatagaagt ttgatatcta tcacttcctg aaattctaga tacagagata ttgtttgatt
     2221 ttatagtatc taaagatttt tcatttatag aagcttctcc accaactaca tatacatttt
     2281 taacttttaa tctctttaat tcagccaaag ttttttcatt tatttgagag cttccagtca
     2341 ataatatagg agcgtttttc ttgtatgcaa atggtgttgc agccaaagca tctgcaattg
     2401 ctgaatcgtt tattaaaact gcattttcag cattttccca acctgcttga cttatcttaa
     2461 tagaagtttc atatctatca gttcctgtaa gtgcacttgc atttacttca tcttgatatg
     2521 taacctcaaa tctagcattt ggacttccta agaaaacaat tacgcctcct tttggcaatc
     2581 taactctatt gttctttgta actaaagaat agtttacagg aacaccttta tcatcataaa
     2641 cagcaaaagc tgaattttga ggaagattaa cttctatttt tttatttgcg atatcgtcac
     2701 caatcttata ccattttgca taaccattgg attctagttc acaagtaaaa gatttttctg
     2761 ctggcaaatt agttatagaa tcttcactaa catacgttag tgttccaaaa gataaatact
     2821 ctgtaccatt ttctttatga agtttaatgt cggataaatc acgacctatt acaccaggta
     2881 tttctataaa agcatttgag ttattctcat ccattatttt agtgttaact atataacctg
     2941 gagtttcatc agaaagagca agagttgcct tcacagaacc aaacatatag ctttgagaag
     3001 tatatttttc atctactaga tagtaatctt taccatttct ttttttccat gcttctttta
     3061 cactatttga tatattgttg tcatctatct tttgagctac atagtataaa cttgctgtct
     3121 gacctaatcc agggacatca tcataagagc tcatatttaa atatgtgata ttgttttttt
     3181 cttttacaaa ttttagacag ctgttacctt tttcagatac aaatctatct tgacctatgt
     3241 atacgtattt atcttcaggt ccattttcta tataaggaga agatactgtt aaagtaccat
     3301 tatcattaac atctactttt atcatatttg aagaagcata aagaccacta ttttctttta
     3361 gagagctagg cattttaact tgttgtggtt tactaaatgt tttatcaggt tttatttctt
     3421 taattttacc tttttctttt aatgcagata gtaagatttc ttgtccaatt atttcattaa
     3481 gttgactact tccaccagaa gaaagaactg caactgccat attttcatct ggaagaacta
     3541 taagattact gtggaataat aaagagtctc caccttttgt aagagctttt aaattatact
     3601 gattaaatgg ataagtgttg acacaatccc accctaaacc atatccaagt atagagtctt
     3661 ctccttcagg ccatagacca tttaaatatt ctttgttttc catagctttt actgaagcag
     3721 gagaaagaat accatttgaa tttttcataa atgtttgtgc aaatgtacat agattttctg
     3781 cacttgaata taaaccacct gcacctatag cattaaggtt atcttgcgga actgcatctt
     3841 cccaataagg cacgtatgct tttgctagtt ttgaactatc aaaactattt tctgtagttt
     3901 ttgtattttg taagttaagt ggattattta tgtatttatc aagaaaatta gtaaatgaca
     3961 taccagatac tctttctact aaaatttcag ctaaagtaaa tccatcatta caataaacgg
     4021 aaaaagctcc tggtttagct tttaatcttt gtttttgtaa ttcttttaag aaattatcat
     4081 gaccataaga atcattgtct gctaataaaa tagtgttttt aaaactactt cccatcaacc
     4141 ctgatgaatg atttaataac attcttggag tgatttcctt gtatctatca tcagccattt
     4201 taaattcagg tatatacttt acaacaggag tatctaaatt taattttcca tcatcaacca
     4261 acttcataac agctgttgta gtaaacattt tactgataga tgcgatacta tacatattat
     4321 ccttatttaa gtttttgtta tcctgtttgc tgtaaactcc accattacca gaaatctcaa
     4381 ttttgccatt atcaatcaaa gcatattgag cacttacagc accatacttt gatagcaaag
     4441 tttggatttt ctctttagca agagctttag tttttgtgta agattccgcg tttttactat
     4501 aatcatgtaa ttctatatca gaactttgat atttttcgga attatctttt gtattatcag
     4561 caaaagtaat atttggagca gcagtaaata acatactcaa tactaatgta ctagataata
     4621 atttagagag cctttgctta aacatattac attacctctt tcccttttta ataatttata
     4681 attagcataa cattcttaat tattttaatc aacagattat tatcaataaa aaaagctttt
     4741 ataagtatag ctttttatat aacagagaaa aatataaaaa taatatttgt tgttgtataa
     4801 agaataaagg gtataggtaa tatataaata aaatggagga attttagaca atgtataaga
     4861 atataaataa aaagcaactt aaagaaatga tgaaaaatga aaaggatgtt ttattattag
     4921 atgtaagaac taaagatgaa tttaaggaat ataaaataga aaatgctata aatatatctt
     4981 tacaagagtt aatcaataat atagatgaaa tttacgatta taaagataag aaagtagtag
     5041 tttattgcag aagtggtcat agaagtgtta ctgcatgtaa tttgttagct gaagaaggtt
     5101 ttgagcattt atataattta aattcaggaa taattgatta tatgagttaa ataatgtaag
     5161 ttatacatta aatactatgt atagctctgc ttattaagta ggaatatatc taaatattta
     5221 taagataaat ttaggaaaga acgaattttt tcgttttttt ctatgacagt ttttgtagta
     5281 aatattagtt tttaagttgg aaaaaggtag tagcttttta gctactacct tttaatatga
     5341 acttagaata ttattaaaaa aaatatattt aaagacagaa ttaatttttt aatgaccagt
     5401 taaattacta tcgccgaatt tatcattaaa tggttttata actctattta taggtccttt
     5461 tagaagtaca tttaaacaaa tagacagaat cataaatagt aatagaacta gtatatcttt
     5521 agttagattt ggagcatata taccaccaac tgcttccctt gtagcaccta ttgcataggt
     5581 aaatggcatt atagggttta ttctttggaa gaatgtaggt gtaacctgta tagggaatgt
     5641 accaccagaa gaaccaatct gaacgactaa tagtactatt gcaattgctt ttcctacatt
     5701 tccaaataca gaaactaaag aatacactat aaagttaaat accatactag taaacataag
     5761 cagaactatc aataaaattg gatgttttgc tgttactcct aacaataata tatctccagt
     5821 tcctattata aaaccttgga caagagctat acttaaaaaa gtaaggcctc ttccaaagta
     5881 aatttcataa ggtttatagt ttccatgtac agatgtagac aatagtgctg aaataagtaa
     5941 tataccaacc catgtagaaa gaacactata aaatggtgtc attgcagaac cataattttc
     6001 aattggatat aatttctctg ttttcacctc aacaggttct tttaagaaat tagactttgt
     6061 caatatatca ctttcaagca agctgactaa ttttttcata tcttcaccat tactaatttt
     6121 gcttaaagta tcaatcaaat catccaacat tccttttgct aaaggaagtc tttctcttat
     6181 caatgatata ctttcctgtg catttcctga aagttttaga gaagtagtaa gtatttcttc
     6241 aacctttgga agttttgctt ctgccttatc taaaacagta attatatcat ttgctacttt
     6301 tatactgttt gcaaatatac tatttatagg ttttgatatt tttgagtcaa aattatttaa
     6361 aatatttaaa ttaattcttc caataccatt tgatagactt aaaacattat tcaaagcact
     6421 tatagatggt tgttgtccag atataacttt gtttttaata tcatttaatg ttgatataga
     6481 tgaatctatt ttattgctag agtcttcaag attatctatt acatcatcca atctattatt
     6541 tgatgtaagt tggtttaatt tagttaaaaa gtccaccaaa gtatcgttta aagattgcaa
     6601 attagaaagt ttttctgata aattatctat caacttaggt acatcttcac tgccactatt
     6661 tactgcatca attaaattta atgtaagaga agaagcagaa gatgaaaggt caaccattaa
     6721 atttaagtca gacttaataa gaggagaaag ttcgtctaaa ttatcatttg tatcctctaa
     6781 gaattttttt aagtcacttg ataatagttt tgtatcattc agagtctttt taattgtagg
     6841 taaatcagat ttaatatctt taactgtatc agataatttt gaagtagcat cagaagctaa
     6901 atcaacagtc ttatttatat ggtcaaatct cttttgaagg tcaataaggg aattttcaac
     6961 ttttgtcaat tttggaagac tgtgttcaat tcctgcacca actccgtttg atataccaag
     7021 aacgatttca ctaactgttt ttactatagt ctgattaacc tgtagttgta ttgtagaagc
     7081 acctttatct gtaatctttg gtgcaactgc atttattttt tcattaacag tatagataat
     7141 ttcgcctttt ttaacatcat ctgtaattaa agatgtcaaa tcttttgtaa aattttttgg
     7201 aatcttaagt gatgcataat aagtaccttt tttaacacct tcaagagcag ttttttcatc
     7261 tacaaactgc caacctaaac tcttgttatc tttgagttgt tcaattaatt ggtctcctac
     7321 attaatcttt tgtcctgcta tttcatttcc tttatcttca tttgttactg ctacaagcat
     7381 attagcagtg cttccataag ggtcccaaga tgcctttata ttgaaccaag catacagaga
     7441 tggtaaaaaa gcaagaccta aaactattac aagtacggca taattgccac gtatatcttt
     7501 taggtcactt ttaaaaattt ctaatatatt ttttaaaccc tttgaaggtc ttaaattctt
     7561 gatattattc cagtttaaaa tttcttttaa actattagtt tttttcaaat gtctcacctc
     7621 ctaaaatatt ttgataaaga aaaaagttag atttataatc
//
LOCUS       DAYAPA010000161         7599 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.166, whole genome shotgun sequence.
ACCESSION   DAYAPA010000161 DAYAPA010000000
VERSION     DAYAPA010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7599
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.166"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(81..293)
                     /locus_tag="ACGI59_003470"
     CDS             complement(81..293)
                     /locus_tag="ACGI59_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFG9741222.1"
                     /translation="MAILMKTKIKEYREKLLMTQNELAKLVGVRRETIVHLENGKYNP
                     SLKLAMDIAKVFDTTVENLFEFIEEE"
     gene            complement(294..710)
                     /locus_tag="ACGI59_003471"
     CDS             complement(294..710)
                     /locus_tag="ACGI59_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9741223.1"
                     /translation="MSKSETGVRSKLILELLVITYFFIAVLATKYIFKGPSTASLPGL
                     SWLIVFGCYSIYNKKYNDLVDESSEFILSKVNKIAIKIMIYSIVIISIFFATPYTSNI
                     NISNLDTGIILIAVLFIQSLSKLVLYIYFDKRGICN"
     gene            complement(778..1191)
                     /locus_tag="ACGI59_003472"
     CDS             complement(778..1191)
                     /locus_tag="ACGI59_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9741224.1"
                     /translation="MSKSELGVKTNLVSELLMISYFFIDSLLKKFIFKEGNLCITFII
                     LFAFWACDFMYNKKYNDLVDEASKLMLLKVDHIASKIMLYSILLIAVFFSANYTRGLD
                     ISNLDIGVILLAILFIQSLSKLILYIHFDRKGICN"
     gene            complement(2024..2653)
                     /locus_tag="ACGI59_003473"
     CDS             complement(2024..2653)
                     /locus_tag="ACGI59_003473"
                     /EC_number="3.2.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase family
                     protein"
                     /protein_id="HFG9741225.1"
                     /translation="MKNRKVYMYVFDTMSDWEVAYLTAELNTGRYFKKGLKPLKVITV
                     GIDKNPIITMGGLKVLPEITVDEFNMESKDLLILPGGNTWLSMNHEIILEKVKEALRQ
                     DSIVAAICGATLGLAKKGLLDFRNHTSNDLEFLKMVIPNYSGEKYYKTESVVSDENLV
                     TASGIAPLEFTFQVLKLLDVFESEALNSWLNLYKTHDSKYFFELMNCNK"
     gene            complement(2732..3694)
                     /locus_tag="ACGI59_003474"
     CDS             complement(2732..3694)
                     /locus_tag="ACGI59_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFG9741226.1"
                     /translation="MAKIDNMMSILWMLNSDKKITAKQIAEKLEINIRTVYRYIDVLS
                     ASGVPIISDTGHNGGYSLLNNFTKAPLFFDIEEQKSLLHAALFAKKEGYFLDEALDKS
                     ILKLKMYSNQKQEDILTNHLRALEVIKPIGKVSIESVLKKLENSILKELSVEINYHAG
                     RKVKSKYRIINPYGIVHWNNNWYVVAFCNMRNEVRSFRVDRISEIIETSNTFNRPTEF
                     SASEFFIKGIIPEIKDEQNIIQLVIEGSIDSLDSLSQHWFLGHYLKERTPNKIIFMLE
                     EEVICKYIPNILLPYRKTIQVIEPLSLRKKIIEDLLELIDYYQI"
     gene            complement(4456..>7599)
                     /gene="cbpA"
                     /locus_tag="ACGI59_003475"
     CDS             complement(4456..>7599)
                     /gene="cbpA"
                     /locus_tag="ACGI59_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding adhesin CbpA"
                     /protein_id="HFG9741227.1"
                     /translation="GDAEGDTNAGDAEGDANAVVVEEEATAEEPTSVGDFKISGGEIN
                     VDFKFASGVLTILSGKQMTIQNNNPNTPTTHRIVIPKDVKAELIFDGVNIKTASNSPF
                     TLTPDKDGGAYAHVILQDGSENKLESGDVYYPGMRAGKGTTLIIDDSVVNRDIEGEFI
                     VPEGGKIPRDVTLENGDKLKKGDRLTLLDSNNRGSLKVVGNYYSAGIGGGNTESGGNI
                     IINGGAITAIGQESSRNTGYGAGIGGGGNGSGGDITINGGIITAKGAYHGAGIGGGYY
                     TTTKNALSPSVVDPSTGSGYSGNITINGGLSYSYGGAHGSAFGDGCVNPGTNRGYRIL
                     VTGGTILPYMTGVGDWIFDLSATTGDVIVTGGSLKATTFKSLGGAVAYGDLEKKTKVF
                     MNKISLTYLGQDKVSTTLVDDFKMLIDGKIYPYGAPSYTDAQGILYLWFSDQFKGSEV
                     SVNLVVIDKITGEVLKPDDFYIKDIGSGSQFLKQYEEFTIDDTPPYLIIKRYDGLPFE
                     EDVEKSFLDWIFKDGGIPIITPKGEKLTDKKFMTISSQRLNEETMEPDINLGSDDLTK
                     QVDAGKYQLSITSTQFSSIYPFKDAYWGHRAYYKAEIIPADTETSLVVEESKAKTDVF
                     KPTDTFTLNVTISPDKKEGPDCASPRGYVQFYINGKEYKDPVELKTHNKGSESHNYST
                     ASIEWRPLDSNHHIFTTEQTITVKYIGDDINYIEGSEDEKKLKLDMTNVDVDGDGIPD
                     INIDTDGDGRPDINIDTSGDWKPDINIDTDNTGEWKPSTEGGNGDGIWKPDKNIDTNG
                     DGNPDTDYNRPAIDTDNDGVDDYWKPDKNVDTGSGGYDTGNPNLNKPDPDNGGNSKPD
                     SGNGGNSKPDSGNGGNSKPDPDNGGNSKPDPDNGGNGKPDPDNGGNSKPDPDNGGNSK
                     PDPDNGGNSKPDIDTDGDGKPNINIDTDGDGKPDINIDTDGDGKPDINIDTDGDGKPD
                     INIDIDGDGKPDINIDTDGNGKPDINIDTDESLDSNVGQNVQTGDQSNIMLDLALMFI
                     SLFFLIKNLTNKYLRRK"
BASE COUNT         2470 a         1365 c          803 g         2961 t
ORIGIN      
        1 tatataaata tttttaacaa acttaataat taaataacta tattttatta tccatcttat
       61 aaatgtttat aaactttcca ctattcttct tcaataaatt caaacaaatt ttctactgta
      121 gtatcgaata ctttagcgat atccatagct aacttcaaag aaggattata tttcccattt
      181 tctaagtgta caatagtctc acgtcttacc ccaaccaatt tagcaagttc attttgcgtc
      241 ataaggagtt tttctctgta ttctttaatt tttgttttca tcaatattgc cacttagtta
      301 catatccccc ttttatcaaa gtatatatac aacactaatt tagataaaga ctgtataaat
      361 aaaactgcaa tgaggattat acctgtatct aggtttgata tatttatatt acttgtataa
      421 ggtgttgcaa agaaaataga tattattact atagaatata tcattatttt tattgcaatc
      481 ttattaacct ttgatagtat aaattcagat gattcatcaa ctaaatcatt atatttttta
      541 ttataaattg aataacatcc aaaaactatt aaccaactca accctggaag agaagcagtg
      601 cttggacctt taaaaatata cttagtggct aatacagcta taaaaaaata agttattaca
      661 agcaactcca ggataagctt tgatcttaca ccagtttctg atttagacat ataaaatcac
      721 tccttaaaaa ttatattctc tatactcttt aattttcgtt tttatcaata ttatcactta
      781 gttgcatatt ccctttctat caaaatgtat atacaaaatt aatttagata aagattgtat
      841 aaataaaata gctaagagaa ttacacctat atccaaattt gatatatcca aacctcttgt
      901 ataatttgct gaaaagaaaa cagctattag caaaatagaa tataacatta tttttgatgc
      961 aatatgatca acctttaata acataagttt tgacgcctcg tcaactaaat cattatattt
     1021 tttattatac atgaagtcac atgcccaaaa agcgaataaa attataaaag taatacaaag
     1081 atttccctct ttaaaaataa acttttttaa taatgagtct ataaaaaaat aggatatcat
     1141 aagtaactct gaaacaagat ttgttttaac accaagctct gatttagaca tataaaatca
     1201 ccccttaaaa gttatatatt tataacacct tatgttataa atatataact tttccaaata
     1261 aattgcaata gaatattgaa aatattcaat aatatcttca taaatatatt ttataaaaga
     1321 ttttataata aacattacaa tacagtacta aattagttta tttcatctat catctcaatc
     1381 atatcattta ctaactatta ttctaatgag catataacat tgaaacttat aattatacaa
     1441 cctaaatgcc tcccaatgta ccctcaaaac atcatttcct gtaactgaag tataattggt
     1501 atattttatc actgaaataa aaaaacacta gtcaataaag actagtattt tcaagtattt
     1561 tatcattctt tttttaatta ttggagacga atcatcagaa taatgcccat aatttatcta
     1621 tatgcaaatt ctaaatttaa aataaagttt cagataaatt tactattcta tgctatgata
     1681 caacagaaaa aactgaaatc tatgagtggt agcttcatat aatcctagtt cctacagtaa
     1741 tcaaaactga ggtttattct atatgtaact aatgataaat tttattatag gagttattag
     1801 atctatttgt gttactataa gtagtaatta tatatatgat aattttttaa cacttttctg
     1861 ttggctatat agaaagtggt ttagaattta aatttaaata aaatttaaat tcaccaaaaa
     1921 gaaatctata ttagaaacca ctactctatt ccaccagatt gtaatttttt gttctttata
     1981 tcacaatgct ataccatatt aaattgaaaa ataagtttta aatttatttg ttacaattca
     2041 ttaattcaaa aaaatatttt gagtcatgag ttttgtaaag atttagccat gaatttaatg
     2101 cttctgattc aaaaacatct aaaagtttca atacttgaaa cgtaaattcc aacggagcaa
     2161 ttcctgaagc cgtaactaaa ttttcatcac taactacaga ttccgtttta taatattttt
     2221 ctccagagta atttggtatt accattttaa gaaactctaa gtcattactt gtatgatttc
     2281 taaaatctaa tagccctttt ttagctaggc ctaatgtagc tccacatatg gctgcaacta
     2341 tagaatcttg tcttaaagct tctttaactt tttctaatat tatctcatga ttcatagata
     2401 accaagtatt tccccctggc aaaattaata aatccttact ttccatatta aactcatcaa
     2461 ctgtaatttc aggtaaaact tttaacccac ccattgttat aataggattt ttatcaattc
     2521 ctacagtaat tacttttagt ggctttaatc cttttttaaa atatcttcct gtatttaatt
     2581 cagcagttaa atatgccact tcccaatctg acatagtatc aaatacatac atatatactt
     2641 ttctattttt catctttttt actccttatg taattaattt atatatctaa ttataaaata
     2701 acttaactga catattcagt cagttaagct attaaatttg ataataatca attaattcta
     2761 gtaaatcttc aattattttc ttcctaaggc ttagtggttc aattacttgt attgtctttc
     2821 tataaggtaa aagtatattt ggtatgtact tgcatattac ttcctcttca agcataaata
     2881 ttattttatt aggtgttctt tctttcaagt agtgccctaa aaaccaatgt tggcttaaac
     2941 tatctaaaga atctatactc ccttctataa ctaattgaat tatattttgt tcatctttta
     3001 tttcaggtat aattcctttt ataaaaaatt cacttgctga aaattctgtc ggtctgttaa
     3061 atgtatttga tgtttctatg atttcactaa tcctatccac tctgaaacta cgaacctcat
     3121 ttctcatatt acaaaatgca actacgtacc aattattatt ccagtgaaca attccatatg
     3181 gatttataat tctatatttt gattttactt tacgacctgc atgatagttg atttctacag
     3241 atagctcctt taatatacta ttctccaatt ttttaagtac tgattctata gacactttcc
     3301 caattggttt tattacttca agggctctta agtggtttgt taaaatatct tcttgttttt
     3361 gatttgaata catttttaac tttaatatcg atttatctaa tgcttcgtct aagaagtatc
     3421 cttctttttt agcaaatagt gctgcatgaa gtagagattt ttgctcttct atatcaaaaa
     3481 aaagaggtgc tttagtaaaa ttatttaaaa ggctataccc accattatga cctgtatctg
     3541 aaattatagg tactccactc gcagaaagta catcaatata tcggtacact gttcttatat
     3601 ttatttctaa tttttcagct atctgttttg ctgtaatttt tttgtcagag tttaacatcc
     3661 acagtataga catcatattg tcaattttag ccatgtagtt tcacctcttt ctatttgtta
     3721 tataaagtta ttatataact ttttgattga ttttttacaa aaaaacatta gtaaactgct
     3781 aataatttat actaagattt atttcaaata tttatagata gtctttttct atagttaagt
     3841 tatccattaa attacatagt agtatatcta ttttagttga tatattttca gctacctccc
     3901 ttgaatatta ttacaataag tctaaatctt ctttcttcca taaatatatt atgaggaaag
     3961 actatcatct ttaatggttt aatagtataa aagatattaa tttaaaaaaa tctattttta
     4021 tatcagatgg gtattgtttt ttataaaact gaaaaaatca cctaatagaa tctatgattt
     4081 tttaacataa cctccataaa ttagttccaa ttatcacttc aaattcaaat ttttatctga
     4141 agaatataca atcatatttc catactcaac ataatattga gtgtttggct tgtatttaca
     4201 aattattatt ctaacaaaaa caactataca tagattaaaa tcatcttcta aacataacta
     4261 ctaaaattga attaatgata tcaattccat taatctcttt taataaaaaa ttctcttttc
     4321 tatatgaaaa ttatttcaaa acttttaagt taaatacaaa aatgactata aatagatttt
     4381 tatttacaca aatctattta tagtcactat agtttatcca ataaaataat aaaataaata
     4441 tgtacacaat aaatgttatt tacgtcttaa gtatttattt gttagatttt taattaaaaa
     4501 aaacaagcta atgaacatta atgctaaatc tagcattata ttgctttgat ctcctgtttg
     4561 tacattttga cctacattac tatctaaact ttcatctgta tctatattta tatctggttt
     4621 tccattccca tctgtatcta tatttatatc cggttttcca tctccatcta tatctatatt
     4681 tatatctggt tttccatctc catctgtatc tatatttata tctggttttc catctccatc
     4741 tgtgtctata tttatatctg gttttccatc tccatctgtg tctatattta tattcggttt
     4801 tccatcccca tctgtatcta tatctggttt gctatttcct ccattatctg ggtctggttt
     4861 actatttcca ccgttatctg gatccggttt gctatttcct ccattatctg gatctggttt
     4921 accattgcca ccgttatctg gatctggttt gctatttcct ccgttatctg gatccggttt
     4981 gctatttcct ccattacctg aatccggttt gctatttcct ccattacctg aatctggttt
     5041 gctatttcct ccattatctg gatctggctt atttagattt ggattacctg tatcataacc
     5101 tcctgagcca gtatctacat ttttatcagg cttccaataa tcatctactc catcattatc
     5161 tgtatctatt gctggacgat tataatctgt atcagggttt ccatctccat tggtatctat
     5221 atttttatca ggtttccaaa taccatctcc atttccacct tctgtactag gcttccattc
     5281 acctgtatta tcagtatcta tgttaatatc tggcttccaa tcaccactcg tatctatatt
     5341 gatatctggc cttccatcac catcagtgtc tatatttata tccggtattc catccccatc
     5401 tacatctaca tttgtcatat ctaactttaa cttcttttca tcctcactac cttcaatgta
     5461 atttatatca tctccaatat attttacagt aatagtttgt tcagtagtaa aaatatgatg
     5521 attgctatct aaaggtctcc attcaataga tgcagtacta taattatgac tctcactacc
     5581 cttattatga gtttttaatt ctactggatc tttatattcc tttccattaa tatagaattg
     5641 tacgtaacct cttggagagg cacaatctgg tccttctttc ttatctggtg aaatagtaac
     5701 atttaatgta aacgtatctg ttggtttaaa aacatcagtt ttagctttag actcttctac
     5761 aactaatgaa gtttctgtat cagcaggaat aatttctgcc ttataatatg ctctatgccc
     5821 ccaataagca tccttaaaag gatatatact cgaaaactgt gttgatgtaa tgcttaattg
     5881 atatttccca gcatcaactt gttttgtaag atcatcactt cctaaattta tatctggttc
     5941 catcgtttcc tcatttaaac gttgagaact aatagtcata aattttttat ctgtgagttt
     6001 ttcaccttta ggagtaatta ttggaatacc accatcttta aatatccaat ctaaaaatga
     6061 tttttctacg tcttcttcaa atggtaatcc atcatatctt ttaataatca aatatggtgg
     6121 agtatcatca atagtaaatt cttcatattg ctttaaaaat tgagaacctg aacctatatc
     6181 tttaatataa aaatcatctg gttttagaac ttctccagta attttatcta taacaactaa
     6241 attaacagat acttcagatc ccttaaattg atcagagaac cataagtaaa gaatcccttg
     6301 agcatctgta taactaggtg ctccataagg ataaattttt ccatcaatta gcattttaaa
     6361 atcatcaact agtgtagtac taactttatc ttgccctaaa tacgttagag atattttatt
     6421 cataaaaact tttgttttct tttctaaatc accgtatgca actgctccac ctaaactttt
     6481 gaatgttgtt gcttttaatg aaccacctgt tactataaca tctcccgttg tagcacttag
     6541 gtcaaaaatc caatctccca ctcctgtcat ataaggtaaa atcgttcctc cagttacaag
     6601 tatacgatat cctctatttg ttcctggatt aacacatcca tccccaaatg cactaccatg
     6661 tgcacctcca tatgaatagg aaagtccacc attaattgta atattcccac tataaccaga
     6721 acctgtgcta ggatctacta ctgagggact taatgcattc tttgttgttg tatagtatcc
     6781 tccacctatt ccagctccat ggtatgcacc tttagcagtt ataatacctc cattgattgt
     6841 gatatctcca ccactaccat taccgcctcc acctattcca gctccatatc ctgtattacg
     6901 actcgattct tgacctatag cagttatagc accaccatta ataataatat ttcctccact
     6961 ttcagtattt ccaccaccaa tacctgcaga ataataattt ccaacaactt ttaaacttcc
     7021 tctattatta ctatctagta gtgtaaggcg atctcctttt tttaacttgt ctccattttc
     7081 tagagttaca tctctaggaa tttttccacc ctcaggaact ataaactctc cttcaatatc
     7141 tctatttaca acagaatcat caataattag agtagtacct tttcctgctc tcatacctgg
     7201 ataatataca tcacctgact ctagcttgtt ttcactacca tcttgtaata taacatgtgc
     7261 atatgcacca ccatctttat ctggtgttaa agtaaatggg ctattagatg cagttttaat
     7321 atttactcca tcaaatatta attcagcttt tacatcttta ggtataacaa tcctatgtgt
     7381 tgttggagta tttggattgt tattttgaat tgtcatttgt tttccactaa gaatagttaa
     7441 aacgccactt gcaaatttaa aatcgacatt tatttctcca ccactaattt taaagtctcc
     7501 tacagacgta ggctcttctg cagtggcttc ttcttctaca actactgcat tagcatctcc
     7561 ttctgcatct cctgcattag tatctccttc tgcatctcc
//
LOCUS       DAYAPA010000162         7456 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.167, whole genome shotgun sequence.
ACCESSION   DAYAPA010000162 DAYAPA010000000
VERSION     DAYAPA010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7456
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.167"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            226..396
                     /locus_tag="ACGI59_003476"
     CDS             226..396
                     /locus_tag="ACGI59_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9741228.1"
                     /translation="MSALLYGVCNKSISVSIVINMQEFKIGVSMAYGVLVIGICYIAL
                     GIIFKLDRKRFT"
     gene            939..1817
                     /locus_tag="ACGI59_003477"
     CDS             939..1817
                     /locus_tag="ACGI59_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFG9741229.1"
                     /translation="MLINMKEMLKVAQENQFAVPAFNIGSGQILKAVVQSANEKNAPV
                     ILAIHPNELSFLGDSFVASCIEEANKSKVPMVIHLDHGENKEQILRAIRCGFTSVMID
                     GSHLPYEENVAISREIVEIAKGLNVSVEGELGTIGTTGTSSEGGTDEIIYTDSKLAKD
                     FVEKTGVDTLAIAIGTAHGIYPKGFKPELKLDLLKEIREVVDIPLVLHGGSSNPDEEI
                     AQAVKLGVCKINISSDVKSAYYKKCRELLEQNPSLYEPDTIYPPCIKAAREVIEFKMN
                     LFNAIDKLKCFYKNKN"
     gene            1853..3748
                     /locus_tag="ACGI59_003478"
     CDS             1853..3748
                     /locus_tag="ACGI59_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFG9741230.1"
                     /translation="MIGKILRSDCIVLDKQLQTKKDVINYMANHLYHGNYINNKEKFI
                     ADINKREKESSTNMGNLIAIPHAKSNVVNQPVMMYLRTDREITDNDGQKLRSFFMIAV
                     PESGSDEHLKIISKLATYLMEENFLKHLLSAETPYEILSIFNNKAAESGDFSNNQNKG
                     LIVGITGCPTGIAHTFMAKKALEEAGQELGYEVKIETHGAIGVENKLTKEDIKRAEYV
                     IIACDKEVQLSRFIGKKVLQKKVVDAKNVSLAKNLIINAENGMAKEIKPGGKTLEDSK
                     EMLQGSEKKGVYKHLMSGVSEMIPFVVIGGIGIAIAFMFGIFASDPTHETYNPIAGFF
                     GQLGGDSAFKLFIPILAGFIAKSIAGRQGFAPAMIAGFMAVIGGSGFIGGMVAGFATG
                     YIALGVMKITENLPDVLKGVNAVLICPLLIAFTSGAIMFFVVNTPASWLNEALQSWLQ
                     SMSGTNKVLLGAILAGMMASDMGGPINKTASAFGLAMFSSNIFEPSAALMVGGMVPPL
                     AISLAMLIFKNKFTKEERKSSLPTAIMGASFITEGAIPFAAADPLRMIPANIIGSCIG
                     GAICMSLDITLMAPHGGIFVIPFACSAPVAYIACILIGTVITALIIGVTKPTIRSENS
                     EVELLGA"
     gene            4016..5923
                     /locus_tag="ACGI59_003479"
     CDS             4016..5923
                     /locus_tag="ACGI59_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFG9741231.1"
                     /translation="MLVSRQIEFLKLILNENKYRPIKYFKDKLNVSDKTLQKDLKMIE
                     KYLETFNIKIDIKRGYGILIEDLAKKNIDLINSLNIQSKENKSLSVEQRRMEILKYLL
                     LNSNNITSINQLADKYYVSKTSIVNDFKYIEAWIKEYNLKLNKTLEGTKIIGKEVDIR
                     KSIAKMMDDLLDENYEQHDISELTRLDSATFSALINLFDIDSIIFVETIITELEYNLG
                     YTINQPYYINLITHILICLKRIEEGNQIESQEEREISVDNLDELVYKNVTTLIDKIEN
                     RYRVKITKDEIMYIYIFLVSSGFSSENNSSYNDESDRLSESEKVSSIMIENMSRFLNI
                     NLKNDDLLQKSLASHVRPMLNRLRYDIQIKNPLLGEIQERFSDVLGLCMMTINIMTEY
                     DNLKNISIDEVSYLATYFQAAIERNMSSKRVIVVCHTGYGTSQLLAARLKREFPEWTI
                     VDIVPMHQLSKRNLDDVDFVISTVNLDLKDKLHIVVSVLLMESDINNIKNALLSKPKK
                     SMNLDTNLLGMYLEDNIYFNFDVAQMESLIGVNLGSKYESISLGKDLNIYIHKQSKLN
                     KGIMNINNVTRTIDIYLEISNQSFLKNILMEISNLYRQKNYINYIIDCKSKEDIKILF
                     KNNLLEKTNGN"
     gene            5913..6362
                     /locus_tag="ACGI59_003480"
     CDS             5913..6362
                     /locus_tag="ACGI59_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFG9741232.1"
                     /translation="MEIREILTEESIILDLKSTTKEGIIQELVDKLYDTGSITLKSKF
                     IKALKHREKIGSTSIGYQMAMPHGKTKYVKKASIVIGKSKKGVDWPSIDGLPVKVIIM
                     FAMPQSGGNEDMILISKIAYILGGKNIGERLLNAHTKKEILDIFYEV"
     gene            6462..7349
                     /locus_tag="ACGI59_003481"
     CDS             6462..7349
                     /locus_tag="ACGI59_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFG9741233.1"
                     /translation="MAIYLEMPKLIHEFPFRTLSNEGEVLTTPHWHKEIEILLITEGV
                     VNLFINDKPMQLKKGEIVIIKGGDIHYILPSPGSERLVFQFDISFFNDLILLNEEKES
                     LINLFSSIKKCSRDWSAKIRKSVFDILIDIYNEDLYKIEGYSYAIKGKMYNLIPILYR
                     QIPREIEENTVEYEINSKEILERLNNIFKYVEKNYKQPIKLEDVSYEVGFSVYYFTKF
                     FKKNTGKTFITFLNEYRLEKAKWILLNNNISIVDLVEEVGFGSTKTFYRVFKEKIGVS
                     PLEFKKRYNSLEKKGSVSK"
BASE COUNT         3061 a          758 c         1296 g         2341 t
ORIGIN      
        1 atatttataa gattatgtta aggaaacact taaaaattag attaaaattg tgggtattta
       61 aatcaactaa aagatattga ctatttagtc aatatctttt tttattaaaa aggaatatac
      121 atataaatag gttagattaa aaattgggat aaatatgatt tatatagcat catttaatta
      181 aaaggagcta tattagtatt cattcataca attggataat atacaatgtc agccttgctt
      241 tatggtgttt gtaataaatc tatttcagtt tctattgtaa ttaatatgca agaatttaag
      301 ataggagtat ctatggctta tggtgtatta gtaattggta tatgctatat agcattagga
      361 ataattttta aattagatag aaaaagattt acataagata acaaaggcta atttataaat
      421 tggtgcttag aaaaccaaat cactttatta gctagagatg aatttggagg gtgtcacttt
      481 ggtgttttat gtaaagttta tggaagtaaa acattagata atcgtgagta agatttctat
      541 aaatcgtata tactattgga atccaagaaa aggtattggc caaatagcta atatcttttt
      601 aatttttttg actctaatat agcattaact attgcatgag tatctttaaa tttatagtta
      661 atgtatctta attgtttatt ataaaatcta ggaaggctaa agcataaaac acttaaaaag
      721 agaagatgta catgagaaat ataattaact atgatgcaga cttctaaaaa ttatttagtg
      781 aaaaggaact tagagtgtat aataaaagtt ccttttcact aaggaaaacg attgttattt
      841 tctgacattc attaccctga tattatttaa atataaacaa caacaaaatt aacaagaagc
      901 aaattcaacc taaaatttaa aatatcggga ggtacaatat gttaataaac atgaaggaaa
      961 tgctaaaagt ggcacaagaa aatcaatttg ctgtaccagc atttaatata ggaagtgggc
     1021 aaatacttaa agcagtagta cagagtgcta acgaaaaaaa tgcaccagta atacttgcaa
     1081 tacacccaaa tgagctaagt tttctaggag atagctttgt agctagttgc atagaagaag
     1141 caaataagag taaagtacct atggtaatac acttagatca tggggaaaat aaagaacaaa
     1201 tattaagggc aataagatgt ggatttacat cagtaatgat tgatggctca catttaccat
     1261 atgaagagaa tgtagctata tctagggaaa tagtagaaat agcaaaagga ttaaatgtgt
     1321 ccgtagaagg agaacttgga acaataggaa caacaggaac atcaagtgaa ggtggtactg
     1381 atgaaataat atatacagat tcaaaattag ctaaggattt tgttgaaaaa acaggagtag
     1441 acacactagc tattgcaatt ggtacagcac atggtatata tccaaaaggt tttaaaccag
     1501 agcttaaact agatttatta aaagaaataa gagaagtagt agatatacct ttagttcttc
     1561 atggtggttc atcaaatcca gatgaagaaa tagcacaagc agttaaacta ggagtttgta
     1621 aaataaatat ttcgagtgat gttaagtcgg catattataa gaagtgcaga gaactattag
     1681 aacaaaaccc aagcttatat gagccagata ctatatatcc accatgtata aaagctgcaa
     1741 gagaagttat agagtttaaa atgaatctat tcaatgctat agataagtta aagtgctttt
     1801 ataaaaataa aaattaatta tgaggtaaca aattaaaagg aggggagaat ttatgatagg
     1861 taaaatacta cgaagcgatt gtattgtact agataaacaa ttacaaacaa aaaaagatgt
     1921 gattaattat atggcaaatc atttgtacca tggaaattat ataaataata aagaaaagtt
     1981 tatagctgat attaataaac gtgaaaaaga atcatcaact aatatgggta atttaatagc
     2041 tatacctcat gctaagtcaa atgtagtaaa tcaacctgta atgatgtatt taagaacaga
     2101 tagagagatt acagataatg atggtcaaaa acttaggtca ttttttatga tagcagttcc
     2161 agaatcaggt tcagatgaac atcttaagat tatatctaaa ctagctacat atctaatgga
     2221 agaaaatttt cttaagcatc tactaagtgc agaaactcca tacgaaatat taagtatatt
     2281 taataataaa gctgcagaat caggagattt tagtaataat caaaataaag gtcttatagt
     2341 tgggataaca ggatgtccaa caggtatagc tcatactttt atggcaaaaa aagcattaga
     2401 ggaagcaggg caagaacttg gatatgaagt taaaatagaa actcatggag ctataggtgt
     2461 agaaaataag ttgactaaag aagatataaa aagagctgaa tacgtaataa tagcttgtga
     2521 taaagaagtt caactgagta gatttatagg aaagaaagtg ctacaaaaga aagtagtaga
     2581 tgctaagaat gtttcattag ctaaaaattt aattataaac gctgaaaatg gaatggctaa
     2641 agaaattaaa ccaggtggaa agactttaga agattcaaaa gaaatgcttc aagggtcaga
     2701 gaaaaagggt gtttataagc acttaatgag tggtgtatct gaaatgattc catttgtagt
     2761 gataggtgga attggtatag ccatagcttt tatgtttggt atatttgcat cagacccaac
     2821 tcatgaaaca tacaacccta tagctggatt ctttggacaa cttggaggcg attcagcatt
     2881 taagttattt ataccgatac ttgctggttt tatagctaaa tcaatagcag gaagacaagg
     2941 atttgctcct gctatgatag ctggttttat ggcagttatt ggaggttcag gttttatagg
     3001 tggtatggta gctggttttg caacaggata tatagcttta ggagtaatga agataacaga
     3061 aaatttacca gatgtattaa aaggagtaaa tgctgtattg atatgtccac ttttgattgc
     3121 atttacgagt ggagctataa tgttctttgt agttaatacg ccagcatcat ggttaaatga
     3181 agctttacaa tcttggttac aaagtatgtc tggaactaat aaagtattat taggtgcaat
     3241 attagcaggg atgatggcat cagatatggg aggacctatt aataaaacag cttcagcttt
     3301 tggattggct atgttttcaa gtaatatatt tgaaccatca gcagcattaa tggtaggagg
     3361 aatggtacct ccattagcaa tatcattagc tatgttaata ttcaaaaata agtttactaa
     3421 agaagaaaga aagtcatctc taccaactgc tataatgggg gcatctttca taacagaagg
     3481 cgcaataccg tttgcagctg cagacccatt aagaatgatt ccagcaaata taattggttc
     3541 ttgtataggt ggggcaatat gtatgagttt agatataact ttaatggcac cacatggggg
     3601 aatatttgta ataccatttg catgtagtgc accagttgca tatatagctt gtatattgat
     3661 tggtacagta ataacagcat tgattatagg ggtaacaaag cctactataa ggagtgaaaa
     3721 tagtgaagtt gaattacttg gtgcttaaat tataaataat agtaaaaata attaatggaa
     3781 taacaacaag tatatagaat aataataagg atatttagaa tcagttaact atttctaaat
     3841 atccttaatt tttattttga aattatatta tctattatat caatttagta aggtgtatca
     3901 taaattcttt tatgatacag aagtatctta gtaattttac atagttagta aatatgttaa
     3961 aatagtactt aatataaaca tagaaatata taaacatagg tagggagggc taagtatgct
     4021 tgtgtcgagg caaattgaat ttcttaaatt gatactaaac gaaaataaat atagacctat
     4081 aaaatacttt aaagataaat taaatgtatc tgataaaact ttacaaaaag atttgaagat
     4141 gattgagaaa tatttagaaa cttttaatat aaaaattgat ataaaaagag gctatggaat
     4201 attaatagaa gatttagcaa agaagaacat agacttaatt aatagtttaa atatacaatc
     4261 gaaggaaaat aaatctttat cagtagagca gagaagaatg gagattttaa agtatttatt
     4321 gctaaattca aataatataa cttcaataaa tcagttagca gataaatatt atgtaagtaa
     4381 aacttctata gtaaatgatt ttaaatatat agaggcatgg ataaaagaat acaatttaaa
     4441 acttaataaa actttagaag gaactaagat tataggtaaa gaagtggata taaggaaatc
     4501 gattgccaaa atgatggatg atttattaga tgaaaattat gaacaacatg atatatctga
     4561 gttaacaaga ttagattctg ctacatttag tgcattgata aatttatttg atattgattc
     4621 tattatattt gtagaaacta taattacaga attagaatac aatttaggat atactataaa
     4681 tcagccctac tatataaatt taattaccca tatacttata tgtttaaaga gaatcgaaga
     4741 aggtaatcaa atagaatctc aggaagaaag agaaatttct gtagataatt tggacgagtt
     4801 agtatataaa aatgtcacta cacttataga taagatagaa aatagataca gagtaaaaat
     4861 aacgaaagat gaaattatgt acatatatat ttttttggta tcatctggat ttagtagcga
     4921 aaataatagt agttacaatg atgaatcaga tagattaagt gaaagtgaaa aagtttcatc
     4981 tataatgata gagaatatgt cacgattttt aaatataaat ctcaaaaatg atgacttact
     5041 acagaaaagt ctagcatctc atgtaaggcc tatgttaaac agattaagat atgatatcca
     5101 aataaaaaac ccgttgttag gagagataca agagcgattt tcagatgtgc tgggattatg
     5161 tatgatgact ataaatatta tgacagagta tgataatctg aagaatatta gtatagatga
     5221 agtttcgtat cttgccacat attttcaagc ggcaatagaa agaaatatga gtagtaaaag
     5281 agtaatagta gtttgtcata caggatatgg aacatctcag ctccttgcag ccagattaaa
     5341 aagagaattt cctgaatgga ctatagttga tatagttcct atgcatcagt tgagtaagcg
     5401 aaatttagat gatgtagact ttgtaatatc tactgtaaac ttagatttga aagataaatt
     5461 acatatagtt gtatcagtac tattaatgga aagtgatatt aataatataa aaaatgcttt
     5521 actttctaaa cctaaaaaaa gtatgaactt agatacaaac ttattaggaa tgtatttaga
     5581 agataatata tattttaatt ttgatgtagc tcagatggaa agtttaatag gtgtgaattt
     5641 agggagtaaa tatgaatcta tttcattagg aaaagattta aatatatata tacataagca
     5701 aagtaaatta aataaaggga taatgaatat caataatgtt actagaacga ttgatattta
     5761 tttagaaatt agtaatcaaa gttttttaaa gaatatttta atggaaatat caaatttata
     5821 taggcaaaag aactatataa actatataat agattgcaag agcaaagagg atattaaaat
     5881 attatttaag aataatctat tggagaaaac aaatggaaat tagagaaata ttaacagaag
     5941 aaagcattat tttagatttg aaatcaacta caaaagaagg cataatacag gaattagtag
     6001 ataaattata cgatacagga agtataactt taaaaagtaa gtttataaag gcattaaaac
     6061 atagagaaaa aataggctct actagtatag gttatcaaat ggccatgcca catggaaaaa
     6121 caaaatatgt aaagaaggca tccatagtaa taggtaaatc taaaaaaggt gtagattggc
     6181 caagtataga tggcttacct gtaaaggtaa taattatgtt tgctatgcca caatctggag
     6241 gtaatgaaga tatgatatta atatctaaaa tagcttacat tttaggtggt aaaaatatag
     6301 gtgaaagact tttaaatgca catacaaaaa aagaaatatt agatatattt tatgaagtgt
     6361 aggtattgta taaattataa atttgaggtt tttaaaataa aatgtgtatt cttttaaata
     6421 gtatagatta atttttagac actagagagg agaggatata tatggcaata tatttagaaa
     6481 tgcctaaatt aatacatgaa tttccttttc gtactctatc aaatgaagga gaagtattaa
     6541 caacaccaca ctggcataag gaaatagaaa tattacttat aacagaaggt gtagtaaatc
     6601 tatttataaa tgacaagcct atgcaattaa aaaaaggcga gatagtgata ataaaaggag
     6661 gagatattca ttatatactg ccatcaccgg gaagtgaaag attagtattt caatttgata
     6721 tatctttttt taatgattta attttattaa atgaggagaa agaaagctta attaatttat
     6781 tcagtagtat aaaaaaatgt agtagagatt ggtcagcaaa aataagaaag tcagttttcg
     6841 atatattgat agatatctac aatgaagacc tatacaaaat agaaggatat agttatgcaa
     6901 taaaaggtaa aatgtataat ttaattccta tattatatag acagattcct agagaaattg
     6961 aagaaaatac agtagaatat gaaataaact ctaaagagat attagagaga ttgaacaata
     7021 tattcaaata tgtagaaaaa aattataaac aacctataaa attagaagat gtgtcttatg
     7081 aagtaggatt tagtgtatat tattttacta aatttttcaa aaaaaataca ggaaaaacat
     7141 ttataacttt tttaaatgaa tatagattag aaaaagcaaa gtggatatta cttaataaca
     7201 atatatccat tgtagatttg gttgaagaag ttggttttgg aagtacaaaa actttttaca
     7261 gagtatttaa agaaaaaatt ggagtctctc cattagaatt taaaaaaaga tataatagct
     7321 tggagaaaaa agggagtgtc tcaaaatgaa cttagttcat gaggcacttt cttttatatg
     7381 aaatcaaata tattttcagt atttcaacaa tgaaaattcc attcatttat aaagtctaaa
     7441 ttttagttct caatac
//
LOCUS       DAYAPA010000163         7408 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.168, whole genome shotgun sequence.
ACCESSION   DAYAPA010000163 DAYAPA010000000
VERSION     DAYAPA010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7408
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.168"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            39..1016
                     /locus_tag="ACGI59_003482"
     CDS             39..1016
                     /locus_tag="ACGI59_003482"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HFG9741234.1"
                     /translation="MRDKIIEIILNNKSEFISGEELSKQLGVSRAAIWKHMKALKEEG
                     YNIESVNKKGYRLAENPTDLLSPQNIYYKLDTEFIGKNIEHLDTIDSTNEHAKRIAIN
                     SVDGTVILSEHQDMGKGRLGRRWDSKPHEGIWMSIILKPDMEPFKAPFITLIAGASVT
                     KALNNLGANTLIKWPNDVILNGKKICGILTELSAEIERTNYVVLGIGINVKIMDFEQE
                     IADKATSLYKEGYMLSRVDIVRNVLTEFEKLYLDYTVNNSKEKTLKICRDYSAIIDKN
                     IYVLKGDNRELVKCLDINEEGNLLVQRSDGSIKEIISGEVSIRGEKGYV"
     gene            1009..1977
                     /locus_tag="ACGI59_003483"
     CDS             1009..1977
                     /locus_tag="ACGI59_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HFG9741235.1"
                     /translation="MYNNILDIEGLKVGQVEDRNGLTGCTVVICEEGATCGVDVRGSA
                     PGTRETDLLDPVNMVQKVHAVVLAGGSAYGLESTCGVSRYLEEKGIGFDVGVGKVPIV
                     VGAVLFDLGVGSFKCRPDLKMGYRACEVANTDILNQGNYGAGCGATVGKIRGQEYAMK
                     GGIGSYSIKLDNGLIVSALIAVNALGDVYENGKVIAGALNDKKTETIDTYKLMKQGES
                     KGGFSVDNTTIGIIATNAKLTKAGCKKISQMGHDGYAKSIFPIHTPHDGDTIFTISTG
                     EIEADITLVGSLAVEVVEKSIINAVKKADSVENIMAYKDIVKKREI"
     gene            2160..2750
                     /locus_tag="ACGI59_003484"
     CDS             2160..2750
                     /locus_tag="ACGI59_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFG9741236.1"
                     /translation="MMNTSASVRKVNVRKMTIIGVLSAISIMLSMTPLGFIPVGPTKA
                     TIMHIPVIIGAIMEGPIVGAAIGFIFGISSLLNAITNPTATSFVFINPLVSILPRVMI
                     GVLAYYVYQLIIKATNKVYISGLITGAIGSLLNTAGVLGMIYVLYADKYLHAMGKSGS
                     AGKVIMALAAANGVPEAIVGALVVAAVATVLKKSKK"
     gene            2769..3539
                     /locus_tag="ACGI59_003485"
     CDS             2769..3539
                     /locus_tag="ACGI59_003485"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HFG9741237.1"
                     /translation="MLLVFDVGNTNMVLGIYKGDKLVNYWRIKTDREKTSDEYGILIS
                     NLFDYDNVNISDIDDVIISSVVPNVMHSLENFCIKYCKKQPLIVGPGIKTGLNIKYDN
                     PKQVGADRIVNAVAGIEKYGAPSILVDFGTATTFCAISEKGEYLGGTIAPGIKISSEA
                     LFQSASKLPRVELAKPGMTICKSTVSAMQSGIIYGYVGLVDKIISMMKKELNCDDVKV
                     IATGGLAKLIASETKSIDYVDGFLTLEGLRIIYEKNQE"
     gene            3676..4644
                     /gene="dusB"
                     /locus_tag="ACGI59_003486"
     CDS             3676..4644
                     /gene="dusB"
                     /locus_tag="ACGI59_003486"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFG9741238.1"
                     /translation="MKIGNLELKNKVFLSPMAGVTDLPFRLICKEQGCGLLYTEMING
                     KALCYDDENTKKMLKIEEEEHPVAVQIFGSEPEFMGRATEIMNDYSNEILDINMGCPA
                     PKVVKNGDGSALMKNPKLAEEVLRAVVKNSKKPVTLKIRKGWDDNSVNAVEIAKIAED
                     CGISALAIHGRTREQFYTGKADWDIIAEIKKNLSIPVIGNGDVFTIEDSINMLDKTGC
                     DAIMIGRGAQGNPWIFKRINHYMNTGEILPEPTLNEKISTAIKHLKLAVEEHGEYVAV
                     REMRKHIAWYLKGLRNSAKLRDEINKIEDYQEVVSKLEYYMEDSLT"
     gene            4753..5232
                     /gene="greA"
                     /locus_tag="ACGI59_003487"
     CDS             4753..5232
                     /gene="greA"
                     /locus_tag="ACGI59_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFG9741239.1"
                     /translation="MEENKEFLLTQEGYDKLEEELENLKVVKRKEVAERIKVAISFGD
                     LSENAEYDEAKKEQAQVEERILKLENMVRKAVIIDESKIDLNVVTIGSIVKVKDLEFD
                     EEVEYTIVGSTEADPYDGKISNESPVGKALLGRAAKEVVEVQVPDGVAKFEILEIRR"
     gene            5247..6776
                     /gene="lysS"
                     /locus_tag="ACGI59_003488"
     CDS             5247..6776
                     /gene="lysS"
                     /locus_tag="ACGI59_003488"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFG9741240.1"
                     /translation="MKNNQQSNEEAQIQEDLSEVLQVRRDKLKKLQESGRDPFKESRY
                     DRTHYSMDIKDNFDSLEGKTTKIAGRIMSKRIQGKAGFIDIQDQEGRIQSYVRLDAIG
                     EEEYSVFSTYDIGDIVGIEGEIFKTKKGEISVKAKSVVLLCKSLQVLPEKYHGLKDQE
                     LRYRQRYVDLIVNPEVKNAFLIRTKALKALRAYLDDRGFLEVETPILNTIAGGANARP
                     FITNHNTLHIPMYLRIANELYLKRLIVGGFDKVYEMGRMFRNEGMDLKHNPEYTAIEL
                     YQAYADYTDMMEITENVIAHMAEVATGSMIVNYQGTEINFTPPWKRMSMEDCVKEYSG
                     VDFSTINTDEEALEVAREKGIEIKPGMRRGEVINAFFEEFGEDKLIQPTFITHHPVEV
                     SPLSKRNVEDPRRTDRFEAFANKWELANAFSELNDPIDQKGRFIDQLRKRELGDDEAF
                     EMDEDFLKALEVGLPPTGGLGIGIDRVIMLLTNSPSIRDVLLFPTMKPIDNNPNKEEE
                     N"
     gene            7085..7279
                     /locus_tag="ACGI59_003489"
     CDS             7085..7279
                     /locus_tag="ACGI59_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9741241.1"
                     /translation="MFQVINFLCLFCVGFLVLDVMIYFCKDAMDIFEGTTNVNKVKSI
                     NVWADRVRANSYSYENKIAK"
BASE COUNT         2950 a          755 c         1503 g         2200 t
ORIGIN      
        1 aaataagtca tgaaattatg tcatattaga ggtgaaatat gagagataaa ataatagaga
       61 ttatattaaa taataaatct gaatttattt caggagaaga attatcaaaa caattagggg
      121 tgtcacgtgc agctatatgg aaacatatga aagccttaaa agaagaggga tataatatag
      181 aatctgtaaa taaaaaaggg tatcgattag cggaaaatcc aactgattta ttaagcccac
      241 agaatattta ctataaatta gatacagagt ttattggaaa aaatattgaa catcttgaca
      301 ctatagattc aacaaatgaa catgctaaaa ggatagctat aaatagtgta gatgggactg
      361 tgattttaag tgaacatcaa gatatgggga aaggacgatt gggaagaaga tgggattcaa
      421 aacctcatga aggaatctgg atgagtatta tattaaaacc agatatggaa ccattcaaag
      481 ccccatttat aactttaata gcaggagcaa gtgtaacgaa agctttaaac aatctaggag
      541 caaatacatt aataaaatgg ccaaatgatg ttattttaaa tggaaaaaaa atatgtggta
      601 tattaacaga gttatctgct gaaattgaaa gaacaaatta tgttgttctt ggaattggaa
      661 taaatgttaa aataatggat tttgaacaag aaatagctga caaagccaca tcattgtata
      721 aagaaggata tatgttatcc agagtagata ttgttaggaa tgtattaact gaatttgaaa
      781 aattatattt agattatact gtaaataatt ccaaagaaaa aaccttaaaa atatgtagag
      841 attattctgc aattatagat aaaaatatat atgtattaaa aggagataat agagaactag
      901 tgaaatgttt agacataaat gaagagggaa atttattagt tcaaagaagt gatggaagta
      961 taaaagaaat tatttcaggt gaagtttcta taagaggaga aaaaggatat gtataataat
     1021 attttggata tagaaggctt aaaggttggg caggttgagg atagaaatgg tcttactggg
     1081 tgcacagtag taatatgtga agaaggagct acttgcggtg ttgatgtaag gggctctgct
     1141 ccaggtacta gagaaaccga tttattagac cctgtaaata tggttcaaaa agttcatgca
     1201 gtagttttgg ctggaggttc tgcatatggt cttgaatcca cttgtggtgt aagtagatac
     1261 ttagaagaaa aaggaattgg ctttgatgta ggtgtaggta aagtgcctat agttgtagga
     1321 gcagttttat ttgatttagg agtagggagc tttaaatgta gaccagattt aaagatgggt
     1381 tatagagctt gtgaagttgc aaatactgat attttaaatc aaggaaatta tggagcaggt
     1441 tgtggagcca ctgttggaaa gataagaggt caggaatatg ctatgaaagg cggaataggt
     1501 agctattcta taaaattaga taatggtcta attgtatctg cattaattgc tgtaaatgct
     1561 cttggagatg tttatgaaaa tggtaaggtt atagctggtg ctttaaatga taaaaagaca
     1621 gaaacaattg atacatataa attaatgaaa caaggtgaaa gtaaaggtgg atttagtgta
     1681 gataatacta caattgggat aattgcaaca aatgctaagt taaccaaagc tggatgtaaa
     1741 aagatatctc aaatgggaca tgatggatat gctaagtcta tttttcctat ccatacacct
     1801 catgatggag atacaatatt cacaatatca acaggagaaa tagaagcaga tataacatta
     1861 gtaggttcat tggctgtaga ggttgtggaa aaaagtataa taaatgcagt aaaaaaagcg
     1921 gatagtgtag aaaatataat ggcatataaa gatatagtaa aaaaacgaga aatatgatga
     1981 aattagcata tttttcttgt caaattgttg acaatggcaa ctcatcttga taaaattatt
     2041 cttgttagag cgggcatcct acaatggtag cttgactcat agttataaga aaaaacgaaa
     2101 aaaattaaaa aattaagtag tccggcatct taatgagctg gaacggaggt gtatttttta
     2161 tgatgaacac aagtgcatca gtaagaaaag taaatgtaag aaagatgact ataataggag
     2221 tactatcagc tatttctatt atgctatcta tgactccatt gggatttatt ccagttggac
     2281 ctacaaaagc aactataatg cacataccag ttattattgg agctattatg gaaggaccaa
     2341 tagttggggc agcaatagga tttatatttg ggatatcaag tctcttaaat gctataacaa
     2401 acccaacagc aacatctttt gtttttataa atccattagt ttcaatatta ccaagagtta
     2461 tgataggtgt attagcttat tatgtatatc agttaataat taaggctaca aataaggtat
     2521 acatatcagg tttaattaca ggggcaatag gttctctgtt aaatacagct ggagttttag
     2581 gaatgatata tgtcttatat gctgacaagt atcttcatgc aatgggtaaa agtggttcag
     2641 caggaaaagt aataatggct cttgccgcag caaatggggt gccagaagca atagtagggg
     2701 ctttagtagt agctgctgtt gccactgtac ttaagaaaag caaaaaatag aaagaaggtt
     2761 aatgacatat gcttctagta tttgatgttg gaaatactaa tatggtttta ggtatatata
     2821 aaggtgacaa attagttaat tactggagaa ttaaaacaga tagggaaaaa acgtctgatg
     2881 aatatggaat cctgataagt aacctatttg attatgataa tgtgaatata agtgatattg
     2941 atgatgttat aatatcatct gtagttccga atgttatgca ttctcttgaa aacttttgta
     3001 taaagtactg taaaaaacag ccattaatag taggtccagg cataaaaaca ggtctaaata
     3061 taaaatatga taatccaaaa caagttgggg cagatagaat agttaatgct gtagcaggga
     3121 tagaaaagta tggagcacca agtatacttg ttgattttgg aacagcaact acattttgtg
     3181 ctatctctga aaaaggtgaa tatttgggtg gaacaatagc accaggaata aaaatatcta
     3241 gtgaggcgtt atttcaaagt gcgtctaaat tacctagagt agaattagct aagccaggta
     3301 tgactatttg taagagtact gtatcagcca tgcaatctgg aataatttat ggatatgttg
     3361 gtttagttga caaaataata agtatgatga agaaagaatt gaattgtgat gatgttaagg
     3421 ttatagctac aggtggatta gctaaactga ttgcttcaga gacgaaaagt atagattatg
     3481 tagatggttt tttaacacta gaaggattga gaataatata tgaaaaaaac caagaataat
     3541 taatatacag ggaattgggt tatatatcca attccctttt tatgatagat ttctagctat
     3601 atgtggtata ttataatgag gaaataagaa tttaaagtta aatcacaaaa taaaggcaaa
     3661 aataggagaa aaacaatgaa aataggaaat ttagaattga aaaataaagt ttttctatca
     3721 cctatggcag gtgtaacaga ccttccattt agattaattt gtaaagaaca aggatgtggg
     3781 cttttatata cagaaatgat aaatggtaag gcactttgct atgatgatga aaatactaaa
     3841 aaaatgctta agatagaaga ggaagaacat cctgtagcag ttcagatatt cggttcagaa
     3901 ccagagttta tgggaagagc aacagagata atgaatgatt attcaaatga gattctcgat
     3961 ataaatatgg ggtgtccagc tccaaaagta gttaaaaatg gagatggttc agcacttatg
     4021 aaaaatccta agcttgcaga agaggtattg agggcagtag tgaagaactc aaaaaagcca
     4081 gtgactttaa agattcgaaa aggctgggat gataatagtg taaatgctgt tgaaatagca
     4141 aagatagcag aggattgtgg aataagtgct ttagcaatac atggaagaac tagagaacag
     4201 ttttatacag gcaaagcaga ctgggatata attgcagaga ttaagaaaaa tttgagtata
     4261 cctgttatag gaaatggtga tgtatttaca atagaagatt caataaatat gttagacaaa
     4321 actggttgtg atgctataat gataggtagg ggtgctcaag gtaacccttg gatatttaaa
     4381 agaataaacc attatatgaa tacaggcgag attttgccag agccaactct taacgaaaaa
     4441 ataagtactg ccattaagca tttaaaactt gcagtagaag aacatggaga gtatgtagct
     4501 gtaagggaaa tgagaaaaca catcgcttgg tatttaaaag gacttagaaa ctctgcaaaa
     4561 ttaagggatg aaattaacaa aatagaagac tatcaagaag ttgtgtcgaa attggagtac
     4621 tatatggaag actccttgac ataaaaataa tagtgactta taatatagcg cataaattaa
     4681 aaaaagatta taaaattttt aaaatataat taggaaaatt cctaagaaat ttgtgaagga
     4741 gttgtattta taatggaaga aaataaagag tttttattga cacaagaggg atatgataaa
     4801 ttagaagaag aattagaaaa cttaaaggtt gtaaagagaa aagaagttgc agagagaata
     4861 aaagttgcta tatcttttgg tgatttatct gaaaatgctg agtatgatga agctaaaaag
     4921 gaacaagcac aagttgaaga aagaatttta aaattagaaa atatggttag aaaagctgtt
     4981 atcatagatg aaagcaaaat agaccttaat gttgttacaa taggatcaat agttaaagtt
     5041 aaggatttag aatttgatga agaggttgaa tatactatag ttggttcgac agaagcagac
     5101 ccatatgatg gtaaaatatc aaacgaatct cctgtaggga aagcactttt aggtagagct
     5161 gcgaaagaag ttgtggaagt gcaagttcct gatggagttg ctaagtttga aatattagaa
     5221 ataagaagat aatttggagg aaaaaaatga aaaataatca acaaagcaat gaagaagcgc
     5281 aaattcaaga agaccttagt gaagtacttc aagtaagaag agataaacta aaaaaattac
     5341 aagagtcagg tagagatcct tttaaagaaa gtagatatga tagaactcat tattctatgg
     5401 atataaaaga taactttgat tctcttgagg gtaaaactac aaaaatagca ggacgtataa
     5461 tgagcaagag aatacaaggg aaagctggct ttatagatat tcaagaccaa gagggaagaa
     5521 tccaatctta tgtaagacta gatgctatag gagaggaaga atatagtgta ttttcaacat
     5581 atgatattgg agatatagtt ggaattgaag gagaaatctt taaaactaaa aaaggtgaaa
     5641 tatctgtaaa ggctaaatct gtagtattac tatgtaaatc tttacaagtt ttaccagaaa
     5701 aatatcatgg attaaaagac caagaactaa gatatagaca aagatatgtt gacttaatag
     5761 taaatccaga agtaaaaaat gcttttttaa ttagaacaaa agcattaaaa gcattaagag
     5821 catacttaga tgatagagga tttttagaag ttgagactcc aatattaaat actatagcag
     5881 gtggggcaaa tgctagacca tttataacta atcataatac tttacatata ccaatgtatt
     5941 taagaatagc gaatgaattg tatttaaaaa gacttattgt tggtggattt gataaagttt
     6001 atgaaatggg aagaatgttt agaaatgaag gtatggattt aaaacataat cctgaatata
     6061 ctgcaataga attatatcaa gcatatgcag actatactga tatgatggaa ataacagaaa
     6121 atgttattgc acatatggct gaagttgcta caggaagtat gatagttaac tatcaaggaa
     6181 cagaaataaa ctttacacca ccttggaaaa gaatgagtat ggaagattgt gttaaagaat
     6241 attctggtgt agatttttct actataaata cagatgaaga ggcactagaa gttgctagag
     6301 aaaaaggtat agagataaaa cctggaatga gaagaggaga agtaataaat gctttctttg
     6361 aagaatttgg agaagataaa ttaatccaac caacatttat aactcaccat ccagttgaag
     6421 tatctccatt atctaagaga aatgttgaag acccaagaag aactgataga tttgaggcat
     6481 ttgctaataa gtgggagtta gctaatgcat tttctgaact taatgaccct attgaccaaa
     6541 aaggaagatt tatagaccaa ttgagaaaaa gagaattagg tgatgatgaa gcttttgaaa
     6601 tggatgaaga tttcttaaaa gcattagagg taggtctacc tcctacagga ggactaggaa
     6661 taggtataga tagagtaatt atgttattaa ctaactctcc atctataaga gatgtattat
     6721 tattcccgac tatgaaacct attgataaca atccaaataa agaagaagaa aactaagaaa
     6781 tattagcatt ctactaagtt ttaaataaag tttagctatt aaattatcaa taaatagcta
     6841 aattttattt aaaatgtcag tgtggaatgc tttttatatg cttcaaagat tcttttagat
     6901 atttaattta taaattacca acaaacgtta gaaattgata ttagtttttg ttgattgaga
     6961 ctttatattt agaaaatatg attgaaagta atttattatg aaaatattta gaataattta
     7021 tgagaaggta aaaagaataa tttatgagaa tactatatat aaaaaattat tgggggaatg
     7081 tctgatgttt caagtaatta attttttatg tttattttgt gttggatttt tggttcttga
     7141 tgttatgata tatttttgta aggatgcaat ggatattttt gagggtacaa ctaatgttaa
     7201 taaagttaag agtataaatg tttgggcaga tagagtgaga gctaattcat attcttatga
     7261 aaataaaatt gcaaaatagg taaaattata tatgcatgta ttgtttgtaa tatcaagggt
     7321 tatatctatt agttttaatt atatttatta aaaaaaccaa aagttataac cttttttaaa
     7381 ataaaaaaca aaaaaagtat tgacgaaa
//
LOCUS       DAYAPA010000164         7380 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.169, whole genome shotgun sequence.
ACCESSION   DAYAPA010000164 DAYAPA010000000
VERSION     DAYAPA010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7380
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.169"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            552..1043
                     /gene="infC"
                     /locus_tag="ACGI59_003490"
     CDS             552..1043
                     /gene="infC"
                     /locus_tag="ACGI59_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015526092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFG9741242.1"
                     /translation="MNEQIKDKEIRVLSPTGEQLGVMPTKEAQAMANSKNLDLVQISP
                     NANPPVCKIMDYGKFRYEQARKEKEAKKKQKTIVVKEVRLRPGIEQNDLNTKANNAIK
                     FLKKGDKVKVELRFRGRELGHKDIGKEVMLKFLDIIKEFGEPTKAPAFEGNNMVVIID
                     PKK"
     gene            1071..1265
                     /gene="rpmI"
                     /locus_tag="ACGI59_003491"
     CDS             1071..1265
                     /gene="rpmI"
                     /locus_tag="ACGI59_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFG9741243.1"
                     /translation="MPKMKTHRGAAKRLKKTGTGKLKRAKAFKKHILTKKSAKTKMNL
                     RKSTLVSDGDAKRIAQLLPY"
     gene            1321..1677
                     /gene="rplT"
                     /locus_tag="ACGI59_003492"
     CDS             1321..1677
                     /gene="rplT"
                     /locus_tag="ACGI59_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFG9741244.1"
                     /translation="MARVKKAMNARKKHKKILKLAKGFRGSRSKLYRPANTFVMKALK
                     NAYIGRKLKKRDFRKLWIQRINAAARMNGISYSRLMNGLKLSGVEVNRKMLSEMAIQD
                     PEGFAKLAEVAKAKLA"
     gene            1987..2532
                     /locus_tag="ACGI59_003493"
     CDS             1987..2532
                     /locus_tag="ACGI59_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9741245.1"
                     /translation="MNEKLMDCFVNPIKCKLLIEIFSNNRTTAKQLGEKFQDISKATL
                     YRYLNKMLKDDIIKVVEENKIRGTVEKVYAMKLDIADDVNKIIDENSGEEYAQLFTQY
                     MMGFLREFYEYAERKEKDILKDGSGFSVCPVYASIDEIVEVSKKIASILEPLRNNSHE
                     EGRRMHSIGVIITPPKKDEGK"
     gene            2967..4892
                     /locus_tag="ACGI59_003494"
     CDS             2967..4892
                     /locus_tag="ACGI59_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFG9741246.1"
                     /translation="MKIKFVIMPLVIFLMVLFVGCQSTKDTEVKAKDTEIKILGTGDL
                     HSILTDSMVSYVNEEREKNKNLLMVDAGDFYGTQSREMWEWSSGKKLINIKRNGRAEY
                     DDIIKSSKDEVPIVKDMAKLKYDAVVLGDNEFVSNDKQSLDKLVSDFKNNNMPLVSAN
                     IYEQSGENYVQPYVMKNIKTDEGNVKVGILGLTIKEVGETLGLEDFEKDKKARELGEQ
                     ADYKGKLYANDLVEDAKKWIKVMEKESPDIIVAVVHSGEEPKTDRNPGNRIKELATTV
                     DGIDAIVAGHTHEKIDEHKYKNKSGDEVIVTQPGWHGDRVSEINFKLNKENGKWDIKE
                     KSSKLKIIDKEVSIVATADLHSHFSDKLTVDLVNERSNPIEPVVVDAGDFLDPQTDEM
                     TQWYKEWKSIRDNNSDKTISRCPMVMDMNQGMYDAVVLGNHEFVSEKDEFWSDERLLD
                     AVIEDFEQVAISVLSANIYRQSGKNYVKPYIIKDVETSEGNVKVGILGLTIKEVGKGL
                     KNVNLQEQFQYKDKLYANDLVEDAKKWVKVMKKKKPDIIVAVVHSGEEPKNPKHPGNR
                     IKELATTVDGIDAIVASHTHEKIDEHEYKNKSGEKVIVTQPGEHGKYYSKITFKVNKE
                     KGSWTINDKFSKTIEVE"
     gene            5293..7197
                     /locus_tag="ACGI59_003495"
     CDS             5293..7197
                     /locus_tag="ACGI59_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFG9741247.1"
                     /translation="MKIKFIIVASIIFLIVLLVGCQSTKDTEINVLATSDLHSIIPDN
                     LISYVEEERKYDENLLMVDAGDFFDMQSGEMNKWFTGQKLVRFKDGIPEYKKIAEPRE
                     GEVPLAKKMAKLKYDAVTFGNHEFVSNDKQSLDKLVSDFKNNNIPLVSANVYEQSGEN
                     YVKPYVMKNINTDKGTVKVGILGLTIKEVGERSRFKSFEQDKKARELGEQPQYKGKLY
                     ANDLVEDANKWVNVMEKKEKPDIIVAIVHSGEEPKNPKNPGNRIKELATTVEGIDAIV
                     AGHTHEKIEQHTYKNNSGEEVIVTQPGAHGNSISKINFKLDKKNDKWFIKDKYSELKV
                     FGKEVNIITTSGLNPKFSDEMAVNLFNERDRDVQPIFIDTGDFLNTNTKEMTKWAKEW
                     QYNENKGIYKKINEWPIVYLGYDAVVLGSHEFTEDKETFEEKKSTLDYIIENFEEMKI
                     PVLSANIYDESGENYVKPYIIHNVETREGNIKVGVLGLTTTDKDEEDLSNSSLSKDSK
                     TSKIPEETGKLYTNNLVKDASEWVKVMQKESPDVIVAVVHSDNNSTLKNPSSEVKKLA
                     MSVDGIDAIVVGHTKNKVEEHIYKNKSGDEVIVTQSGEDCYSKISLELRKENGKWNII
                     NKYSKAIKMK"
BASE COUNT         3103 a          735 c         1374 g         2168 t
ORIGIN      
        1 atgtaggact tgttatgtag tatagtaaca tattagaata taatgtagga tttgttatgt
       61 agtataatag tatattagag tacaatatag aatttgttat gtagtatagt agtatattag
      121 aatacaatat agaatttgtt gggtagtata gtgttatatt agaatacaat ataggatttg
      181 ttatgtagaa tatatagagt catgtagaat atacatatat aaaaggattt tgggtataaa
      241 tttaaaaatt aatagcaaaa tgagtagtat agacatataa ttagtagtag gaagtaatta
      301 aatttttcct aaacaaaaaa aatagttgca aataatttga ttaagtgata tagtaaataa
      361 ggtaagaata aaacaaagaa gaagtccgct tctcacctta cgccggaggt tagtaaggta
      421 aatataagtt aaagcttaat tttaatttaa gtttgtttaa atttggtaac ttatattgcg
      481 gatgattacg tcatccgctt tttattttat aaaaattttg gaggtgtcct acaattagca
      541 aagaattagc aatcaatgaa caaataaaag ataaggaaat aagagtcctt tcaccaacag
      601 gtgaacaact tggagtaatg cctactaaag aagctcaagc aatggctaat agcaaaaact
      661 tagatttggt tcaaatctcg ccgaatgcaa atccaccagt atgtaagata atggattatg
      721 gaaagtttag atatgagcaa gcgagaaaag agaaagaagc taagaaaaaa caaaaaacta
      781 tagttgtaaa agaagtaaga cttagaccag gtattgaaca aaatgattta aatacgaaag
      841 ctaataatgc tataaagttc cttaaaaaag gggataaagt caaagttgaa cttagattta
      901 gaggaagaga attaggacat aaagacatag gtaaagaagt tatgcttaag tttttagata
      961 taataaaaga gtttggagaa ccaacaaaag cccctgcatt tgagggtaat aatatggttg
     1021 taattataga tccaaaaaaa tagatgatta actgagagga ggaatttgtc atgcctaaaa
     1081 tgaaaactca tagaggtgca gcaaagagat taaagaaaac aggaactgga aagttaaaga
     1141 gagctaaagc ttttaaaaaa catatattaa ctaaaaaatc tgcaaaaact aaaatgaact
     1201 taagaaaatc aactttagta agtgatggag atgctaagag aatagcacaa ttattaccat
     1261 actaagatac agattgtaaa taataactat agaagaggga tataaaagga ggtcataacg
     1321 atggcaagag ttaaaaaagc tatgaacgct cgtaaaaagc ataagaaaat attaaaactt
     1381 gcaaaaggat tcagaggatc tagaagcaaa ttatatagac cagcaaatac attcgtaatg
     1441 aaagcattaa aaaatgctta tataggaaga aagttaaaga agagagactt cagaaaactt
     1501 tggatacaaa gaataaatgc agctgctaga atgaatggaa tatcttattc aagattaatg
     1561 aatggattaa aattatctgg tgttgaagtt aacagaaaaa tgttatctga aatggctata
     1621 caagacccag aaggatttgc aaaattagca gaagttgcta aagcaaaatt agcttagtat
     1681 tgaatcatga gatgtaaagt ctttgagtta tataaataat tcaaggactt ttttattttt
     1741 tgaaaaatac ttattagtgt catgtacaac caattttata atgataaata aaataagcaa
     1801 tacaatagaa ttttatgaga tttattataa gaattgttac aattatttat gatagctata
     1861 aaaagtcata gtaaaaatga tattgatgta tgttgatata tgtataaagt ttccaatcaa
     1921 tcttgaatta atattatcaa catgataata ttatcttatt aataatataa ataaggggga
     1981 cataatatga atgaaaaact aatggattgt tttgtaaacc caattaaatg caagttattg
     2041 attgaaattt tttcaaataa tcgaactaca gctaaacaat taggagaaaa atttcaagat
     2101 atatcaaagg caacacttta tcgttacctt aataaaatgt tgaaagatga tattataaaa
     2161 gtggttgaag aaaataaaat aaggggaact gttgaaaaag tatatgctat gaagttagat
     2221 atagctgatg atgtgaacaa aattatagat gagaactctg gagaagaata tgctcaactg
     2281 tttactcaat atatgatggg atttttaaga gaattttatg aatacgctga aagaaaggaa
     2341 aaggatatcc taaaagatgg ttcaggattt tctgtctgtc cagtatatgc tagtattgat
     2401 gagatagttg aagtgagtaa aaaaattgct agtatactag aacctctaag aaataactca
     2461 catgaagaag gtagaagaat gcattctata ggagtgataa ttactccacc taaaaaggat
     2521 gaagggaaat aaatctgatt tacatggggt agagccaatt tatacaggga atcgttttat
     2581 gatttacata tgtatccaga gcaaagtatc tctgcttgga ttgtacttca ttgtaccatt
     2641 tcatccaatg acttggagtg gtacaatgaa gttttttaat ttttcatatt aatgctaaag
     2701 aaatgaactt tagaccacat atgcaattag gttgcattat acaattagac cacatataca
     2761 aaataaaaat cagtattttt atagcagata aattgactgt cattcttctt aaaaatcatc
     2821 tattagttat tgttagcata ttgaaagaaa ttatagtaat ctattgaata aatgaatatt
     2881 gcctataatc atataaactg ctatagttta aaataaagca ggacgttaaa ttttatagta
     2941 atctaaaaat ttaagagggg gattttatga aaataaaatt tgttattatg ccattggtga
     3001 tatttttaat ggtattattt gtggggtgtc aaagtacaaa agatacagaa gttaaagcaa
     3061 aagatacaga aattaaaata ttagggacag gtgatttaca ttcaattttg acagactcta
     3121 tggtttcgta tgtaaatgag gaaagggaaa agaataaaaa tctgcttatg gtagatgcgg
     3181 gagatttcta tggtacacaa agtagagaaa tgtgggaatg gtcttctgga aaaaagttaa
     3241 ttaatattaa aagaaatggt agagcagagt atgatgacat aattaagagt agtaaagatg
     3301 aagtacctat agttaaagat atggcaaagt tgaaatatga tgctgtagtt ttgggagata
     3361 atgaatttgt ttcaaatgat aagcaaagtc ttgataaact ggtttctgat tttaaaaata
     3421 ataatatgcc attggtatca gcaaatatat atgaacaatc tggagaaaat tatgtacaac
     3481 catatgtaat gaaaaatatt aagacagatg agggcaatgt aaaagttgga atactaggtc
     3541 ttactataaa agaagtaggg gaaacccttg gtcttgagga ttttgaaaag gataaaaaag
     3601 ctagagagct tggagaacaa gctgattaca aaggaaaatt atatgcaaat gatttagtag
     3661 aagatgcaaa aaaatggata aaagttatgg aaaaagaaag tccagacata atagttgcag
     3721 ttgtacactc aggggaagaa cctaaaacag atagaaatcc aggaaataga ataaaagagt
     3781 tagcaactac agtggatgga atagatgcaa ttgtagctgg tcatactcat gaaaaaatag
     3841 atgaacacaa atataaaaat aaatctggag atgaagtaat tgttacacaa ccaggttggc
     3901 atggagatag agtatcagag attaatttca aactaaataa agagaatgga aagtgggaca
     3961 taaaggaaaa gtctagtaaa ttaaaaataa ttgataaaga agtaagtata gttgctactg
     4021 cagatttaca ttcacatttc tctgataaat taacagttga ccttgttaat gaacgaagta
     4081 acccaattga acctgttgta gtagatgcag gcgacttctt agaccctcaa acagatgaaa
     4141 tgactcaatg gtataaagag tggaaaagta taagagataa taatagtgat aaaacgatta
     4201 gtagatgccc aatggtcatg gatatgaatc agggaatgta tgatgcagta gtacttggaa
     4261 atcatgaatt tgtatctgaa aaagatgaat tttggtcaga tgaacgcctt ttagatgctg
     4321 ttatagagga ttttgaacag gtggcaattt ctgtgttatc ggcaaatata tataggcaat
     4381 ctggtaaaaa ttatgttaaa ccatatatta taaaagatgt ggagacgagt gaaggtaatg
     4441 taaaagtagg aatattggga cttactataa aagaagtagg taaaggtctt aaaaatgtaa
     4501 acttacaaga gcaatttcaa tataaggata aattgtatgc aaatgattta gtggaagatg
     4561 caaagaaatg ggtaaaagtt atgaaaaaga aaaaaccaga tataatagtt gcagttgtac
     4621 attcaggaga agaaccaaaa aatccaaaac atccaggaaa tagaataaaa gaactagcaa
     4681 ctacagtaga tggtattgat gcaattgttg ctagtcatac tcatgaaaaa atagatgagc
     4741 atgaatataa aaataagtct ggagaaaaag ttatagtgac acaaccaggt gagcatggta
     4801 aatattattc taaaattacc tttaaagtaa ataaagaaaa gggttcgtgg actattaatg
     4861 ataaattcag taaaacaatt gaggttgaat aactttataa aatttataga taaagtaaat
     4921 tatctgatat tggcatgata tataagataa agtttctaat attagtataa tgcataatgt
     4981 gaaatatttt aataataaga gaataaacaa gacaaataaa aatattttaa catgtaagat
     5041 atagtatgaa ctattatact aatgcttact ttataatttg aacatgttag aataagtctt
     5101 atttgaaaat tttagtgtac tagagtaagt attattttac tagtaaaaaa tattgagaac
     5161 tatatgtaga tttgcttaaa aaggaattta catatagttc ttttcgtata ataaatagta
     5221 caatcttata tggtaaagat aaagtgtgat attaaatttt attatcatcc aaaatctaag
     5281 gaggagtctc ttatgaaaat aaaatttatt atagtagcat caataatatt tttaatagta
     5341 ttacttgtgg ggtgtcaaag tactaaagac acagaaatca atgtactcgc cacaagtgat
     5401 ttacattcaa ttattccaga taatttaatt tcctatgtag aagaggagag aaaatatgat
     5461 gagaatctac ttatggtaga tgcaggagat ttttttgaca tgcaaagtgg tgagatgaat
     5521 aagtggttta ctggtcaaaa attagtaaga tttaaagatg ggataccaga gtacaaaaaa
     5581 atagctgaac cacgtgaagg agaagttcca ttggctaaaa aaatggcaaa gctgaagtat
     5641 gatgcagtta catttgggaa tcatgaattt gtatctaatg acaagcagag ccttgataaa
     5701 ttggtttctg attttaaaaa taacaatata ccattagtat cagcaaacgt atatgaacaa
     5761 tctggagaaa actacgtaaa gccatatgta atgaaaaata taaatactga caagggaact
     5821 gtaaaggttg gtatacttgg tctgactata aaagaagtag gggaacgttc aaggtttaaa
     5881 agttttgaac aagataaaaa ggctagagaa cttggggaac aacctcaata caaagggaaa
     5941 ttatatgcaa atgatttagt agaagatgca aataagtggg taaatgttat ggaaaaaaaa
     6001 gaaaagccag atataatagt tgccattgta cattcaggtg aagagccaaa aaatcctaaa
     6061 aatccaggaa atagaataaa agaactagca acaactgtag agggaataga tgctattgta
     6121 gcaggtcata cacatgaaaa aatagaacag catacatata aaaataattc tggtgaagaa
     6181 gtaatagtaa cacagcctgg agctcatggt aatagtatat caaagattaa ttttaagttg
     6241 gataagaaga atgataagtg gtttataaaa gacaaatata gtgaattaaa ggtttttgga
     6301 aaggaagtaa atataattac aacttctggt ctgaatccaa aattttcaga tgaaatggca
     6361 gttaatttgt ttaatgaaag agatagagat gttcaaccta tctttataga tactggagat
     6421 tttttaaata ctaatacaaa agaaatgact aagtgggcta aagagtggca atacaatgaa
     6481 aataaaggca tctataaaaa aataaatgaa tggccaattg tatatcttgg ttatgatgct
     6541 gtagtacttg gtagccatga atttacagag gataaagaga cttttgagga aaaaaaatct
     6601 acattggatt atattataga aaactttgaa gaaatgaaga taccagtatt atcagctaat
     6661 atatatgatg aatctggaga aaattatgta aaaccttata taattcataa tgtagaaact
     6721 agggaaggaa atataaaggt tggagtatta ggtcttacta ctactgataa ggatgaagaa
     6781 gacttaagta actcaagctt aagcaaagac tcaaagactt cgaaaattcc tgaagaaaca
     6841 ggaaagttat atactaataa tctagtaaaa gatgcaagcg aatgggtaaa agttatgcaa
     6901 aaggaaagtc ctgatgtgat tgttgcagtt gtacattcag ataataatag tactctaaaa
     6961 aatccaagta gtgaagtcaa aaaacttgca atgtcagtag atggaataga tgctattgtt
     7021 gtaggtcata cgaaaaataa ggtagaagaa catatttaca aaaataagtc tggggatgag
     7081 gttatagtaa cacaatctgg agaagattgt tattcaaaaa taagtcttga actaagaaaa
     7141 gaaaatggca agtggaatat cataaacaaa tatagtaaag caataaaaat gaagtgaatt
     7201 tattgatata taaaccatta ttaacactga tttttataat ataataaata ctgattctac
     7261 aatataatta acactgattt ttataatata ataaatactg attctataat ataattaaca
     7321 ctacttttta taatgtcatc aacactgatt ctataatata attaatacta cttttttaaa
//
LOCUS       DAYAPA010000165         7350 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.170, whole genome shotgun sequence.
ACCESSION   DAYAPA010000165 DAYAPA010000000
VERSION     DAYAPA010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7350
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.170"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            173..1384
                     /locus_tag="ACGI59_003496"
     CDS             173..1384
                     /locus_tag="ACGI59_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFG9741248.1"
                     /translation="MKGLVLEGGGTKGAYQIGAYKALRELGIEFQGVTGTSIGALNGA
                     YIVQNDIEVMEDIWLNYDYTHFMNIDRETYERFKNVDFTTKNLNAVFELINKARKNEG
                     IDISPLRQLMETTLNEDIIRKSKKDFGLVTVWWDKKINPYPLYIEDIPKGRLIDYLIA
                     SSSLPIFQTNMMDDKLYLDGMFCDNMPIGLLEDKGYEDVVVIRLIDDFIGKMNLNRHQ
                     NINIKTIIPSEYLGGSLNKDRDNVEKNIRLGYLDTMKAYERCTGIKYYFDVEYKYNEE
                     YCFDKFRSMSNETIQYICNLLNIKRDASLRTVMESVIPKLGEVLGLPKDFSYKDLFYC
                     IYEKKLEENNINRIKLYDFNKVIDVVNTNIDSNRNLASNFEVRPKISIKKNKNTKLLT
                     NCIIKDIKNQS"
     gene            1577..1837
                     /locus_tag="ACGI59_003497"
     CDS             1577..1837
                     /locus_tag="ACGI59_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HFG9741249.1"
                     /translation="MEKVVSIKNASGLHARPAGMFVKKAAEFKSTVEVVAKDKTVNAK
                     SIMGIMSLGLAQGEELKLVVNGEDEEVALAAMVELVESGFGE"
     gene            2191..3903
                     /gene="ptsP"
                     /locus_tag="ACGI59_003498"
     CDS             2191..3903
                     /gene="ptsP"
                     /locus_tag="ACGI59_003498"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFG9741250.1"
                     /translation="MAYKGIGASPGVALGKALVVEHSELVIEKKSIDNVEAEIAKLED
                     AVAVSKEELVKVKEKASEELGAEEAEIFEAHLLVLEDPELIGSAIDKIKTESVNAEYA
                     LNEIKEMFVSMFESMDNEYMKERAADIKDVTNRILRHILGIKVVDLSALSEEVVLIAH
                     DLTPSDTATMNKKMVLGFLTDIGGRTSHTAIMSRTLEIAAIVGLNDITSKVKDGDFVV
                     FNGDTGEVIVNPDEETINKYTELKAKYEDERKALQLLKGKPSVTLDGKHVELAGNIGT
                     PNDIEGLIKNDAEGVGLYRTEFLYMDRDSFPTEEIQYEAYKAVLEGMDGKPIVIRTLD
                     IGGDKELSYLSMEPEMNPFLGYRAIRLCLDRKDIFKTQLRALYRASVHGRLRIMFPMI
                     SSLEELLQAKEVVKEVLAELDSEGVAYAKDVEIGMMIEVPSAAVISDVLAKHVDFFSI
                     GTNDLIQYTCAVDRMNQKISYLYNQFNPAVLRLIKTVIDNAHKEGKWAGMCGESAGDQ
                     KMIPILLGMGLDEFSMSPISILPARKLITSVKESDMKKLADDVLNMGTAEEIKSYIEK
                     TFNI"
     gene            complement(4695..5360)
                     /locus_tag="ACGI59_003499"
     CDS             complement(4695..5360)
                     /locus_tag="ACGI59_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9741251.1"
                     /translation="MKGNIVQYNFADIEEEVYSLDYAIAWNTDEENVNIIPFTNKFCK
                     ESIESFCLGKINNFVEILNEGFVENHHYVHLDKMISVPKKKVNLVYQQDTHGYLLRDD
                     NDNLIPAKITSEQSKSISSKMELFCAGEEKCLINILLKADPSYILDVDSIKDKNILNL
                     GYESIDRYKEYNFDDDKILIFFINKKRYSVIMKKTNNSDNDLVSRNNAIKELFTNKAG
                     NLN"
     gene            5704..7134
                     /locus_tag="ACGI59_003500"
     CDS             5704..7134
                     /locus_tag="ACGI59_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HFG9741252.1"
                     /translation="MDIKNKIKSIFLPEKNDYLDDEEFEYDIENNEEDLKEDNKVHLN
                     DLSRVKYDYRELKDIENQTEEMCLLAVKQDGTIIEFVKDKTYKVCMEAVKQTYKSLKY
                     ITNQNEDICIEAVKQNYRALYYINNKTENVLIEAIKNASTYDVMEVFKFVEEQTEDVC
                     LAFIERASKNDVAEILKGIKEQTPAICLEAVKKDGKSLAYVKEQSNSICLEAVKENYS
                     ALSCVKEQTEEICIEAVKQNDFALYYVNEQTEKICMEAVKRSYMALQYVNKQTEEICL
                     EAVRIDGRALQYVKEQTEEICLESVRQNGKVLQYVKKQTENICIEAVRGSFEELEIKE
                     ILSYVKIPTERIFVEAVKQNGKILKYVENQTELICLEAVRENYNALAYVKEQTEKICL
                     EAVNQSYEALKYVKEQTEEVCLKAVKQDYRMLKYVNNQTEKICLEAVKQNYRALEFVD
                     NQTEKVCLEAVKQNRKALQYVKQKQS"
BASE COUNT         3043 a          714 c         1238 g         2355 t
ORIGIN      
        1 aaagttagcc atataaaaaa tattaaaaat atatactttt caatattttt ttgataaaat
       61 aagaatgaat ttgtgcaatt ttatttttct ccaataacta aaaatagttt tttgaattaa
      121 tatttgctta agaataaaaa tcagaaacgt ttttgatata gtaggtgata atatgaaagg
      181 attagtttta gaaggtggag gaacaaaagg agcctaccaa ataggtgcat ataaagcttt
      241 aagagagctt ggtatagagt ttcaaggagt aactggcaca tcaattggtg ctttaaatgg
      301 agcatatata gttcaaaatg atattgaagt aatggaagat atttggttaa attatgacta
      361 tactcatttt atgaatattg atagagaaac ctatgagagg tttaaaaatg tagatttcac
      421 tactaaaaat ctcaatgcag tgtttgagct tataaataaa gctagaaaaa atgaaggtat
      481 agatataagc ccattaagac aactaatgga gacaacatta aatgaagaca taataaggaa
      541 atcaaaaaaa gactttggac ttgttacggt ctggtgggat aaaaaaatta atccatatcc
      601 attgtatatc gaagatatac ctaaaggaag attgattgat tatttaattg ctagttccag
      661 tttgcctatt tttcaaacga acatgatgga tgataagttg tatcttgatg gaatgttttg
      721 tgataacatg ccaataggac tcttggaaga caaaggttat gaagatgttg ttgtaattag
      781 gttgattgat gattttattg ggaaaatgaa tctaaatcga catcaaaata taaatataaa
      841 aactataata ccatcagaat atctaggagg ttctcttaat aaagatagag ataatgtaga
      901 aaaaaacatc cgactaggtt acttagatac tatgaaagca tatgaaagat gcactggaat
      961 taagtattat tttgatgtag agtataaata taatgaagaa tattgttttg ataaatttag
     1021 aagtatgagc aatgaaacaa tacaatatat ctgtaattta ttgaatataa aaagagatgc
     1081 ttcacttaga accgttatgg aaagtgtaat accaaagctt ggtgaggtgc ttggattgcc
     1141 aaaggatttt tcatataaag atttgtttta ctgtatatat gaaaagaaac ttgaagaaaa
     1201 taatataaac aggataaaat tatatgattt taacaaggtt atagatgttg taaatacaaa
     1261 tattgattct aatagaaatt tagcttctaa ttttgaagtt agacctaaaa ttagtattaa
     1321 aaagaacaaa aacacaaaat tattgacaaa ttgtattata aaagacataa aaaatcaaag
     1381 ttaaataaaa aacaaaaaaa aatttgtaat tatactcgaa aaaagtgtat attagtagta
     1441 taatattttt gtagcactag tgggaacaaa aaatgaccat aggacaaaaa agtaccactt
     1501 agtaccatta aagtaaaaaa caggaaaaca attttaaaaa atataaaatg taaagtttat
     1561 aggaggattt aaaaaaatgg aaaaagtagt tagtatcaaa aatgcaagtg gattacatgc
     1621 tagaccagca ggaatgtttg ttaagaaggc agctgaattt aaatcaacag tagaggttgt
     1681 agcaaaagac aaaactgtaa atgcaaaatc aataatggga ataatgagtt taggtttagc
     1741 tcaaggagaa gaattaaagt tagttgtaaa tggagaagat gaagaagtag ctttagctgc
     1801 aatggttgaa ttagttgaaa gtggatttgg agaatagata taataatttt ttaaaaattt
     1861 gatatttaga atctaatatt acttaaagta aagtaacaga gaagtattaa acatatacag
     1921 accaacattt gtagatattt aatttctttg ttactttcat gtttatttaa tcagaagatt
     1981 aagactattg ttaattgggt ataaaaatat aaatatttta gaaaatgaaa taagatattt
     2041 ttaatcataa attcagagta aaaatacagt aacaaatagt ttataaaatt ttataaaaaa
     2101 ttataagtaa ataaaactat aaaaatgtaa attatttata taaatttata aagtttatgt
     2161 gctagatgat attttttagg aggtagggtt atggcttaca aaggaatagg tgcttctcct
     2221 ggtgttgctt tagggaaagc gttagtagtt gaacatagtg aattagtaat tgaaaaaaaa
     2281 tctatagata atgttgaagc tgaaattgct aaattagaag atgcagttgc agtttctaaa
     2341 gaagaattag taaaagttaa ggaaaaggct tctgaagaat taggagcaga ggaagcagaa
     2401 atatttgaag cacatttatt agttttagaa gacccagagt taattggttc tgcaatagat
     2461 aaaattaaaa ctgaaagtgt aaatgcagag tatgctttaa atgaaataaa agagatgttt
     2521 gttagcatgt ttgagtctat ggacaatgag tacatgaaag aaagagcggc agatataaaa
     2581 gatgttacaa atagaatact tagacatata ttaggaatta aagttgttga tttatctgct
     2641 ttaagcgaag aagttgtatt aatagcacat gatttaactc catcagacac agcaactatg
     2701 aataaaaaaa tggttttagg atttttaact gatataggtg gaagaacttc tcatactgct
     2761 atcatgtcta gaactttaga aatagctgca atcgttggat taaatgatat aacttcaaaa
     2821 gttaaggatg gagattttgt ggttttcaat ggagacacag gtgaagtaat cgtgaatcct
     2881 gatgaagaga ctataaataa atatactgaa ttaaaagcta agtatgaaga tgagagaaaa
     2941 gcattacagc tattaaaagg aaaaccatct gtaactttag acggaaaaca tgtggaatta
     3001 gctggtaaca taggaacacc taacgatata gaaggtctaa taaagaatga tgctgaaggt
     3061 gttggtcttt atagaacaga atttttatac atggatagag attctttccc aacagaagaa
     3121 atacaatatg aagcatataa agctgtatta gaaggaatgg atggaaagcc tatagttata
     3181 agaacattgg atataggtgg agataaagaa ttaagttatt taagtatgga acctgaaatg
     3241 aatccattct taggttacag agcaataaga ctttgcctag atagaaaaga tatatttaag
     3301 actcaattga gagctttata tagagctagt gttcatggaa gattaagaat catgttccca
     3361 atgatttcat ctttagaaga gttgttacaa gctaaagaag ttgtaaaaga agttttagca
     3421 gaacttgatt ctgaaggtgt ggcttatgct aaagatgtag aaattggtat gatgatagaa
     3481 gttccatctg ctgctgtgat atctgatgta ttggctaaac acgtagattt cttctctata
     3541 ggaactaatg atttaattca atacacttgt gcagttgata gaatgaatca aaagataagc
     3601 tatctttaca atcaatttaa tccagctgtt ttaagactta taaagactgt tatagacaat
     3661 gcacataaag aaggcaaatg ggcaggtatg tgtggagaat ctgctggaga ccaaaaaatg
     3721 ataccaatac ttttaggtat gggattagac gagttctcta tgtctccaat atcaatactt
     3781 cctgctagaa agttaataac ttcagttaaa gagtcagata tgaaaaaatt agctgatgat
     3841 gtacttaata tgggtactgc tgaagaaata aaaagctata tagaaaagac ttttaatata
     3901 taattaatgt tatatagtta ctaaaattca tttttctaaa taacaaaatt atattttacg
     3961 aattaaaaag gagatgtttc aaaataaaaa tttgaagcat ctctttttta taaaagatta
     4021 ctaagaatta cttaatcttt gtaatagtca ttttgtgaaa aagcaaaact gcattgtggc
     4081 tatagtatat atggatgtta ttatcaataa aagtactatt aaaatgaaga ttcattctca
     4141 aatgatatca aaagtagttg tgatggagta agataatatt ttgtgattta atagtaaaaa
     4201 ttaaaaaaat atggtattga aaaataatta aaagtattta ataatagaat tagggaagaa
     4261 taagtttata agtaaaaata attgattact agttaataaa ataaatgtga tatccaaata
     4321 aatcctaaat ctaatttgag ttttaatata gatagtctaa agtttgatta ttaaagattt
     4381 aattgtaagt tgttacagtt gatgatattg attagagatt aaagtttaga gtagaaagtt
     4441 accttaaatt taaaaatgca agataaccca atctaaaaat tataatcact caataggttt
     4501 aaggatttat ttgtttatta taaataggag ggtgtctcaa aatgaacttt ttagtttatg
     4561 aggcactctc ttttatatag aattaaatat attttattat ttcaacaatg aaaaagaagc
     4621 ttatctctat tttgagacag cctctttgta tactattatt aatttaatca ataacataat
     4681 ttaaaactat attattagtt aagatttcct gctttattag taaataattc tttaatagca
     4741 ttgtttctgg aaactaaatc gttatcagag ttgtttgttt ttttcataat cactgaataa
     4801 cgttttttat ttataaaaaa tattaaaatc ttatcatcat caaaattgta ttctttatat
     4861 ctgtcaatag actcataacc tagatttaga atgtttttgt cttttatact atctacatct
     4921 aaaatatatg atgggtcagc ctttagtaat atattaatta aacatttttc ttcaccagca
     4981 cagaataact ccatcttact tgatatagat ttagattgct ctgaagttat ttttgcagga
     5041 ataaggttgt cattgtcatc tcttagaaga taaccatgtg tgtcttgttg atatactaaa
     5101 ttaacctttt tcttaggaac actaatcatt ttatctaaat gtacataatg atgattttca
     5161 acaaaacctt catttaatat ttcaacaaag ttattaattt tacctaggca aaaagattct
     5221 atagattctt tacagaattt atttgtaaat ggtattatgt ttacgttttc ctcgtctgta
     5281 ttccatgcta tagcataatc caacgaatat acttcttcct ctatatctgc aaaattatat
     5341 tgtactatgt ttcctttcaa aattactcga cctccatctt ataatatatt taatatactt
     5401 tataaaactt atattatatt taaataatat cataaattga ataaatatga tatagtaaat
     5461 aagaaaaaat ttgaaaaaaa tcactataaa tgagtaataa atataaatag tgtatactaa
     5521 tgggatggaa aatgtccact attaacttta ttaccccaca tatataagat aaaattatta
     5581 atataaaata taatctatcg acaatgaata gacttttaaa taaacttaat ataaaattaa
     5641 ttaaataagt gcttaaaaac gatttacttt aatgatagaa tcatattaaa ggaggataat
     5701 tttatggata taaagaataa aattaaatct atatttttac cagaaaaaaa tgactattta
     5761 gatgatgaag aatttgagta tgatatagaa aataatgaag aagatttaaa ggaagataac
     5821 aaagtacatt taaatgattt aagcagagta aaatatgatt acagagagtt aaaagacata
     5881 gaaaaccaaa cagaagagat gtgtctgcta gcagtaaagc aggatggtac tataattgag
     5941 tttgtaaagg acaagactta taaagtatgt atggaagcag taaaacagac atataaatct
     6001 cttaagtata taacaaatca aaatgaggat atttgtatag aagcagtaaa acagaactac
     6061 agagctttgt actacataaa taataagaca gaaaatgtac taattgaggc tataaaaaat
     6121 gcatcaactt atgatgtcat ggaagtattt aaatttgtgg aagagcaaac agaagatgta
     6181 tgtctagcat ttatagaaag ggcatctaaa aatgatgttg cagagatatt aaaaggaata
     6241 aaagagcaaa ctccagccat atgtttagaa gctgtaaaaa aagatggaaa atctttagct
     6301 tatgtaaagg aacaaagtaa tagtatatgt ttagaagctg taaaagaaaa ctacagtgca
     6361 ctatcttgtg taaaagagca aactgaagaa atatgtatag aagcagtaaa acagaatgac
     6421 tttgcattgt attatgtaaa tgaacagaca gaaaaaatat gcatggaagc agtaaaaaga
     6481 agttatatgg ctctacagta tgtaaataag caaacagaag agatttgttt ggaagcagta
     6541 agaattgatg gaagagcatt acagtacgta aaagagcaaa cagaagaaat ttgcttggag
     6601 tcagttagac aaaatggaaa agtactgcaa tatgtaaaaa aacagactga aaatatatgt
     6661 atagaagcag taagaggttc atttgaagaa cttgaaataa aagaaatatt aagttatgta
     6721 aagataccaa cagaaagaat atttgtagaa gcagtaaagc aaaatggaaa aattcttaaa
     6781 tatgtagaaa atcagacaga attaatatgt ttagaggcag taagagaaaa ctataatgct
     6841 ttagcttatg taaaggaaca aacagaaaag atttgtttag aagcagttaa tcaaagttat
     6901 gaagcgctta aatatgtgaa agaacaaaca gaagaagttt gtctaaaagc agtaaaacaa
     6961 gattatagaa tgttgaaata tgtaaataat caaacagaaa aaatatgttt agaagcagta
     7021 aagcaaaatt atagagcttt agaatttgtt gataatcaaa cagaaaaagt ttgtcttgaa
     7081 gcagtaaaac agaatagaaa agcactacag tatgtaaaac aaaaacaaag ttaaaaggct
     7141 aaaatatatg gggaatatat gcaaataaat aaaatttgat attttgtaat aaataaaatg
     7201 ggtatctcaa aaggaacttt ttagtttgtg aggtactctt tttatatgaa atgaaatata
     7261 tttttagcct tttaacaaag gggaaaaggt ttgtttctat tttgacatag gctttttatg
     7321 catataaagc ttgataaaat tgttttatta
//
LOCUS       DAYAPA010000166         7326 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.171, whole genome shotgun sequence.
ACCESSION   DAYAPA010000166 DAYAPA010000000
VERSION     DAYAPA010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7326
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.171"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(313..1152)
                     /locus_tag="ACGI59_003501"
     CDS             complement(313..1152)
                     /locus_tag="ACGI59_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFG9741253.1"
                     /translation="MSDIKNEVSKKTLSKKDVIKSWLRWFFFAQSNYNYERLQSTAFS
                     HSMLPVLKKLYPDKEELKQEVETHLAFFNTEPICGCVIHGITIAMEEEKANGADEISG
                     DGMNAIKTGLMGPLAGIGDTLTQGVITPIVLAVCIGLTEGGASIAGPILFVIAQYIIM
                     TSISFGMWTNGYKYGKKAVESILQGGIVNKVIEGASILGTLVMGGLVGRFINLSTPIS
                     YTSGDFKFSLQTDLLDKIFPGLIPLVLTLLVLFALKKGLSPIKIMFILIVVGAITGIL
                     GLF"
     gene            complement(1176..1934)
                     /locus_tag="ACGI59_003502"
     CDS             complement(1176..1934)
                     /locus_tag="ACGI59_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFG9741254.1"
                     /translation="MHISLIQAVLIAIFYYLSWSPWLTYVGFFTWNRPLLAGFVTGII
                     LGDPVQGAIIGAGINMIYLGFISAGGAQMGDPAFAGYVGTALAIASKLDVSTAMAIAV
                     PLGTVATVLWIGKMTVNSFFAHWADREVQKGNVDRVAFINIVPPQILLFAMSFIPALL
                     VVYFGPNAIDGMLEVMNDNVLHVFNVIGAMLPALGIAMNLKLIGNKFTMPFFILGILM
                     AVYFKVDIIVISVIGVILALTITSIKYGKDSATS"
     gene            complement(1981..2454)
                     /locus_tag="ACGI59_003503"
     CDS             complement(1981..2454)
                     /locus_tag="ACGI59_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFG9741255.1"
                     /translation="MSNIALTRIDDRLIHGQVITAWCKITSAKRIVIVDDLVVKDPFI
                     VQVLQMAAPSTVKVEVHDVESGAEVLKSHNGDENLIVLVKYPKTVLGLVNSGVDLKEL
                     NVGGMGAGLGRKSFYKNISVSDEEKEIFRELISKDVKCFIQIVPDAKKIDVGTLL"
     gene            complement(2483..2899)
                     /locus_tag="ACGI59_003504"
     CDS             complement(2483..2899)
                     /locus_tag="ACGI59_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFG9741256.1"
                     /translation="MIHMAGIIIATHGNMAKAMLESAELIVGKQENVETLGLNHGDSI
                     DEFSRKLEDGIEKYKDEGVLVLLDFYGGTPFNTSAIVINKFAKVCELECLTGVNLPML
                     LELFLNRDTMKLKDLKNLCEEVGISGIKDVKTVLKL"
     gene            complement(2946..3998)
                     /locus_tag="ACGI59_003505"
     CDS             complement(2946..3998)
                     /locus_tag="ACGI59_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFG9741257.1"
                     /translation="MKSVRFYGIRDTRVEDVDVPKILEKDDVIIKVKVAGICGSDISK
                     YSKTGPHMVGEILGHEFSGEVAQVGKEVRSFKIGDRVAVCPAMPCFECDECKKGLYSR
                     CNNVAIIGNKELGGCFAEYTKVKERNLIKIPDEISYETAAALEPVCIAGHGLFRSEAK
                     VGDTVVVLGTGPIGLFSIQWAKIFGSTKIIAVDVFDEKLDLAKELGADICINAKEKNI
                     VEEIKRLTDGDGADIVIESAGTPLTCGQVLLLAKKGGTVLYAGVPYGDVALTREQFEK
                     IVRSELTVKGTWFGNSFPFPGKEWSAGLYHMQKGDMNVEKLVTHRINLEEVPAYFEKV
                     YKRDIFFGKIMINIDN"
     gene            4394..5074
                     /locus_tag="ACGI59_003506"
     CDS             4394..5074
                     /locus_tag="ACGI59_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFG9741258.1"
                     /translation="MKKVYQGKTKDVFELDNGNYLLKFKDDVTGKDGVFDPGENSVGL
                     SIDGIGRANLETSVKFFEILNNAGIKTHYVSANLEEATMEVLPAKVFGNGLEVICRYR
                     AVGSFLRRYGSCVEEGAKLDCYVEATLKDDDRCDPLITSEGLEALNIMTQAQFDSMKS
                     MTQKISNIVRDTIAEKGLELYDIKFEFGFYNDEVILIDEIASGNMRVYKDGVIVDPMD
                     LTALLLDK"
     gene            complement(5207..5527)
                     /gene="sugE"
                     /locus_tag="ACGI59_003507"
     CDS             complement(5207..5527)
                     /gene="sugE"
                     /locus_tag="ACGI59_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFG9741259.1"
                     /translation="MKWLFLFVAGLFEVFWAFQLKNSHGFSKLTPSILTVVGMIVSFY
                     FLSLALKHLPLGTSYAIWTGIGTVGTAIVGMAILNEPISISRIACIGFIVVGIVGLKL
                     LSTN"
     regulatory      complement(5597..5703)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00442"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="guanidine-I (ykkC/yxkD leader) riboswitch;
                     Guanidine-I riboswitches control genes whose products are
                     involved in modification or pumping out guanidine as a
                     toxic compound from bacteria; This is class of guanidine
                     riboswitches is based on the ykkC/yxkD leader; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="guanidine"
                     /db_xref="RFAM:RF00442"
     gene            5909..6736
                     /locus_tag="ACGI59_003508"
     CDS             5909..6736
                     /locus_tag="ACGI59_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HFG9741260.1"
                     /translation="MNFYKIAVCQMITTENKIENINHAVDMVTEAAINGAKIVVLPEM
                     FNCPYENKYFPKFAEEYPGETTTILSKLAEKHGIYLVSGSIPELEDGKIYNTCYVFDK
                     NGALIGKHRKMHLFDIEVTGKVSFKESDTLTAGNDVTVIDTEYGKVGIAICYDIRFPE
                     LSRLMALKGAEIVILPAAFNMTTGPAHWELSIRMRALDNQIFYVGAAPARNMNASYIA
                     FGNSRISDPWGRIIAQADEKECIIYADIDRDLIPDIRQQLPLLKHRRTDLYELNTLK"
BASE COUNT         2490 a         1316 c          846 g         2674 t
ORIGIN      
        1 ttaacaatat ctcgattata tattttaata tgtaactaat atgttataat attttaatta
       61 aattaaataa atttatattt ttttgaaatt acttaatatt ttatataaaa atgaaaaagc
      121 acctcattct gattaaaaat ctttctgaga tactttttaa aatattaaat actacatata
      181 gcttacataa gtttaaaatt taatctatta taaaattacc ctaattgcca aactttttaa
      241 tataaatttt ccaacatcct acaaatataa tcaatagata tagaatccta acttacacca
      301 atatatattt atttaaaata gtcctaaaat acctgtaata gctcccacta ctatcagtat
      361 aaacattatt tttattggag aaagaccttt ttttaatgca aagagtacta ataaagtaag
      421 taccaaagga attaatcctg gaaatatctt atcaagtaaa tccgtttgaa gactaaattt
      481 aaaatcacca ctagtataac ttattggtgt agataaattg ataaacctac ctacaagccc
      541 ccccatgact aaggtaccta gtatagaagc cccttctata accttattta ctattccacc
      601 ttgaagtata ctttcaacag cttttttccc atacttatat ccatttgtcc acattccaaa
      661 agaaattgaa gtcattatta tatattgagc tattacaaat aatataggtc ctgcaattga
      721 tgctcctccc tcagttaaac caatacaaac agctaacact ataggagtta taactccttg
      781 agttaaagta tctccaattc ccgctagtgg acccatcaag cctgttttga tagcattcat
      841 accatctcca cttatttcat ctgctccatt agctttttct tcttccatag ctattgttat
      901 tccatgtata acacatccac atataggttc tgtgttaaaa aatgctagat gagtctccac
      961 ttcttgtttt aactcttcct tatctggata taatttttta agtactggca acattgaatg
     1021 tgaaaaagct gttgattgca atctttcata attgtaatta gattgtgcaa agaaaaacca
     1081 tcttaaccat gattttatta catctttctt gcttaacgtt ttcttagata cttcattttt
     1141 tatatctgac ataatatttt ccccctcaca tgtatttaag atgtagcact atcttttccg
     1201 tacttaattg aagtaattgt aagagcaagt attactccaa taacagatat tactattata
     1261 tctactttaa aataaacagc cattagtatt cctaatatga aaaagggcat agtaaactta
     1321 tttccaatca attttaaatt catagcaatc ccaagtgctg gcaacatagc cccaattaca
     1381 ttaaatacat gtaacacatt atcattcatt acttccaaca taccatctat cgcatttgga
     1441 ccaaaataaa caactaataa tgctggtata aaactcattg caaataacag tatctgtggt
     1501 ggtactatat ttataaatgc tactctatca acattccctt tttgaacctc tctatcagcc
     1561 caatgtgcaa aaaaagaatt tactgtcatc tttccaatcc aaagtacagt tgcaacagtt
     1621 cccaatggta cagctattgc cattgcagta cttacatcta gcttagatgc tatagctaaa
     1681 gcagtaccta catatcctgc aaaagctggg tcccccatct gagctccccc tgctgatata
     1741 aaacctagat atatcatatt tatacctgct cctataattg ctccttgaac agggtctcct
     1801 aatataattc ccgttacaaa tccagccaat agaggtctat tccatgtaaa aaatccaaca
     1861 taagtcaacc acggactcca agaaagataa taaaaaattg ctattaatac tgcttgaatt
     1921 agtgaaatgt gcatattctt cctccctatt ttcttttaaa gatttacaag atagtcttct
     1981 ttacaataat gttcctacat caattttctt tgcatctggt actatttgta taaagcattt
     2041 aacatctttt gatattagct ctctaaatat ttctttttct tcatctgaca cagatatatt
     2101 tttataaaaa ctttttctac caagccctgc tcccatacct ccaacattca attcctttaa
     2161 atctacccca ctatttacta aacctaagac agttttaggg tatttgacca atactattaa
     2221 attttcatct ccattatgtg attttaagac ttcagcacct gattctacat catgtacttc
     2281 aaccttaaca gttgatggag ctgccatttg taaaacttga actataaatg ggtcctttac
     2341 tactaaatca tccacgatta ctatcctttt tgctgatgta atcttacacc aagcagtaat
     2401 aacttgccca tgtattaatc tatcatcaat tcttgttaat gctatattac tcataattat
     2461 tcctccagat ttttaattta ttttacaact ttaaaacagt tttaacatct ttgattcctg
     2521 atatcccaac ttcctcacat aaattcttca agtcttttaa cttcatagta tctctgttta
     2581 aaaataactc taaaagcata ggtaaattta cacctgtcaa acattctaac tcacatacct
     2641 tagcaaattt atttattaca atagcagatg tattaaatgg agttccgcca taaaaatcaa
     2701 gcaatactaa aaccccttca tccttatatt tctcaattcc atcttctaac tttctactaa
     2761 actcatctat gctgtcacca tggtttaatc ctaaagtttc tacattttcc tgtttgccta
     2821 ctattaattc agcactttct aacattgctt tagccatatt tccatgtgtg gctataatta
     2881 ttcctgccat atgaatcata cctttcttaa ccttttttat atggattatt atttagtact
     2941 ctaatctaat tatctatatt aatcattatc ttaccaaaga aaatatccct tttgtatact
     3001 ttttcaaaat aagctggtac ttcttctaaa tttattctat gtgttactaa tttttcaaca
     3061 ttcatatcac ccttttgcat atgatacaat ccagcactcc attcttttcc tgggaatgga
     3121 aaggagtttc caaaccaagt tccctttaca gttaattcac ttcttactat cttttcaaat
     3181 tgctctctag tcaaagctac atccccatat ggtacacctg catacaatac tgtacctccc
     3241 tttttagcta gcaacagtac ttgaccacat gtcagtggag taccagccga ttcaataaca
     3301 atatctgcac catctccatc agttaacctt tttatttctt ctacaatatt cttttctttt
     3361 gcatttatac aaatatctgc ccctaactct tttgccaagt ctaacttctc atcaaataca
     3421 tccacagcta ttatttttgt agaaccaaat atttttgccc attgtattga aaataatcct
     3481 attggacctg taccaagtac tacaacagta tctccaactt tagcttctga cctaaaaaga
     3541 ccatgtcctg ctatacatac tggttctagc gcagcggcag tctcataact tatctcatct
     3601 ggtattttta ttaaatttct ctctttcacc tttgtatact ctgcaaaaca acctccaagt
     3661 tctttatttc ctattattgc tacattatta catcttgaat acaagccttt tttacattca
     3721 tcacattcaa aacatggcat agctggacaa acagcaactc tatctcctat tttaaaactc
     3781 cttacttctt taccaacctg tgccacttct ccagaaaatt catgccctaa aatttctcca
     3841 accatatgtg gacctgtctt tgaatatttt gatatatcag aaccacatat tccagctact
     3901 tttactttaa ttattacatc atctttttct aatatttttg gtacatccac atcctccact
     3961 cttgtgtctc ttataccata gaatctaact gacttcacta ttaaccaccc ctacttttta
     4021 tttgagaata tatccctatt gtttatatgt taaatatcta aagcaacttt catgccaata
     4081 tatgtaagca ttgtattcat gcattttata aaattatcat taaatgtgtg tcaaagtgtg
     4141 tcaaaagttt cttatattaa aacttatgtg tgtcaatata aacgacacta acattttata
     4201 ttagttctct tattataagt tttataaaat gtactttttt tgttattttt ataaaattgt
     4261 tcggaaattt cccttctatt tgtgatataa tattaaatat aaggtatcaa tataaatttt
     4321 catgcgaata tcagaacttt tttgacacta atgccttatt caatataata atttaacttt
     4381 ttggaggttg tttatgaaaa aagtgtatca aggaaagact aaagatgttt ttgaattaga
     4441 taatggaaat tacttactta aatttaaaga cgatgtaact ggtaaagatg gagtatttga
     4501 cccaggcgaa aatagtgtag gtctaagtat agatggaatt ggtagagcta atttagagac
     4561 atctgttaag ttctttgaaa ttctaaacaa tgctggaatt aaaactcatt atgtaagtgc
     4621 taatttagaa gaagcaacta tggaagttct accagcaaaa gtttttggta atggattaga
     4681 agttatttgt cgttatagag ctgtaggtag tttcttacgt cgttatggtt catgtgttga
     4741 agaaggtgct aaactagatt gttacgtaga agcaacttta aaagatgatg accgttgtga
     4801 ccctcttata acatctgaag gtttagaagc tttaaacatt atgactcaag ctcaatttga
     4861 ttcaatgaaa agcatgactc aaaaaatttc taacatagtt cgtgatacta tagctgaaaa
     4921 aggtcttgag ctttatgata ttaaatttga atttggattc tacaatgatg aagtaatctt
     4981 aatagatgaa atagcatctg gaaatatgcg tgtttataaa gatggtgtaa tagttgaccc
     5041 tatggattta actgctcttt tattagataa atagtatata catataattt acaaatataa
     5101 taaacttata aataagataa gctatgaagt aaagactttt taaatgttga ttatttttaa
     5161 acattttctc gcctttaatt tcatagctta tttaaattaa tatactctaa ttagtagata
     5221 atagctttaa ccctacaatt cccaccacaa taaaacctat acatgcaatt cttgatatac
     5281 taataggctc atttaaaata gccatgccta caatagctgt accgacggta ccaattccag
     5341 tccatattgc atatgaagtt ccaagtggaa gatgttttag tgctaatgac aaaaaataaa
     5401 aacttacaat cattccaaca acagtaagta tacttggagt taatttagaa aatccatgtg
     5461 aattttttag ttgaaaagcc caaaacactt caaacaaacc tgcaacaaac aaaaataacc
     5521 atttcataaa ttcatccctc ttttcaaata tgtctcttat ttttccaata aaataacctg
     5581 agaaatatta taaaatatct cccaggtttt tgtccttccg tgttcacaac aaaaactatg
     5641 ttgtgggttt tatctcggac cagaccaatc attaataatt gcggaaccct ataaaactct
     5701 attaattatt ctattttatc tataataata ctatcaacat atataaaaaa agtcaatata
     5761 tgggttccat atataataaa aataactttt atataccttt taatattctt attcacttct
     5821 ctccataccc atttgggtca atctaagaat atctaatttt tatttaatat gttataataa
     5881 cataaaaata ttgaaaggag taatttctat gaatttctat aaaattgcag tatgtcagat
     5941 gattactaca gaaaataaaa ttgaaaatat caatcatgct gtagatatgg taacagaagc
     6001 tgccatcaat ggagctaaaa ttgtagtttt gccagaaatg tttaactgcc catatgaaaa
     6061 taaatatttt cctaaatttg ctgaagaata tccaggtgaa acaacaacga tattaagtaa
     6121 attagctgag aaacatggaa tttatctagt ttctggtagt attcctgagt tagaagatgg
     6181 aaaaatctat aatacttgct atgtatttga taaaaatgga gctctaattg gaaaacatcg
     6241 taaaatgcac ttatttgaca ttgaagttac aggaaaagtt agttttaaag aatcagatac
     6301 tcttacagct ggaaatgatg tcactgtaat agataccgaa tatggaaaag ttggtatagc
     6361 tatttgctat gatattcgtt tccctgagtt atcacgttta atggctctta aaggtgctga
     6421 aatcgttatc ttacctgctg catttaatat gactactggt cccgcccatt gggagttatc
     6481 tataagaatg cgtgctttag ataatcagat attctatgtt ggtgctgcac ctgctagaaa
     6541 tatgaatgct tcttatatag cttttggaaa ttcaagaatt tcagaccctt ggggaagaat
     6601 aattgctcaa gctgatgaaa aagaatgtat tatttatgct gacattgata gagatttaat
     6661 tcctgacata agacaacagt taccacttct taagcacaga agaactgatt tatatgaact
     6721 taatactttg aaataaaaca gaacataacc ccataaatca taaattaagt gttaaaaaaa
     6781 tttatggggt tctcattaat ttgatttaaa attaatttca taaaaataat gctaatatta
     6841 atttaattaa ataatactaa taattactaa tgattatgct aattatcagc atttactaat
     6901 ttaatcacta ttagttattt cttttattaa atatctttac ttttttactt attatgtctt
     6961 ctagctttac atttggatta tggtatttct ttctattttt agtcacatct ttctattgaa
     7021 ttacttgctt aaaacaccaa tgcacacatg ccagataatg ttcacaatta tttagaaatc
     7081 ttgctctacc ttcttcaatg ttgatattat ctctcctagt tgatattatc tttcctaaaa
     7141 tttcttttaa ttaaaaaaca acattagttg atatatcaag tatattttaa gcaactgaaa
     7201 aaaacaataa taattgatat atcaactaat taatgtagat taattcattt ttataattta
     7261 tatacttttt aattaatcta ccttatttaa ttaaaatcct tcctttctat ttatccattt
     7321 tcaact
//
LOCUS       DAYAPA010000167         6962 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.172, whole genome shotgun sequence.
ACCESSION   DAYAPA010000167 DAYAPA010000000
VERSION     DAYAPA010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6962
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.172"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(604..1818)
                     /locus_tag="ACGI59_003509"
     CDS             complement(604..1818)
                     /locus_tag="ACGI59_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFG9741261.1"
                     /translation="MRNCIIAQSGGPTAVINASAVGIYEKNLETKYFDKVYFGINGIE
                     GILNKNIIDSDSLNKDNVLNLKQTPSSGLGSCRYKLADYKINSYDYDRVINILDEYKI
                     DTLFYIGGNDSMDTVMKFSQYCKNTTCKIKFIGIPKTIDNDLLGTDNCPGFGSAAKLI
                     STLIMETYLDFSIYKNNGIFIVETMGRDTGWLAASSSLARLNDTPLVDLIYLPEIDFY
                     EDKFIEDVRKIFKEKNKAYIVASEGIRDKYGNFICEQKTSAHDKFGHSQLGGVSNYLK
                     NLIVSSSITTRVKTLELGVIQRCAMHLASDTDLKQAAEVGAYGIELSKKGYTGVMAII
                     NRTCNNPYKYEVSHTDISKIANKTKYFPKEWINEECNYITDEALNYLYPLIQGKPDIK
                     YKNGLPYFSIIY"
     gene            2244..2960
                     /locus_tag="ACGI59_003510"
     CDS             2244..2960
                     /locus_tag="ACGI59_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFG9741262.1"
                     /translation="MKHIKIAAGLAHVNYGHISAVVKEAMDAGVDYVHSDAADMHDLK
                     NMQLMGGHQIIEAIRPVTDKPIECHIYTRDCDRLFIEKIAAAGCNLLIIPAEHFLGAP
                     LAYIINYCREFGMKVGLTIGCYTPLCFVDEAIYDIDRLQIVVHGVDETDGKDNWGWRK
                     SAVDLVKRARKMIDEKNPKCELAIDGGLRADNMEPLIECNPDVVILSSAIFKDKDGIT
                     AGVKNCRKAIDEAATKFGLE"
     gene            3095..3721
                     /locus_tag="ACGI59_003511"
     CDS             3095..3721
                     /locus_tag="ACGI59_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFG9741263.1"
                     /translation="MKKEMIKDYLLITISAFLMALAVNFFFAEHTLAPGGITGLSVVL
                     STFLSMPVENISLGISMPLLVMGLLFLGKGFGIKTLYITLMGPLFLKVIPQTHITDNL
                     LVAGVIGGLLVGTAIGIAIIRGCSTGGTDLVAMLINKVIKFLKLPVILFMLDGFIIVA
                     SGIISKNFMISIYSLISLLLIIKTIGFVTTKFGKNTEEINNINNACAQ"
     gene            complement(3921..5465)
                     /locus_tag="ACGI59_003512"
     CDS             complement(3921..5465)
                     /locus_tag="ACGI59_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFG9741264.1"
                     /translation="MNKRLLDIAELLLNSSDYITVDTIAEELKVSNKTIRNDLVILDE
                     WLLEFNLSLDKKTGSGVIILGNEDIKLKVIRDINDKSNCIYAYSPEDRKRYILSKLFI
                     KNSKFRIRDICNELHVSRATVHKDLVVIQNFLQNFKVTLVRKTNNGVYLEGKEKDIRK
                     AIFELATVNKSYTELKEILFSKSNECKDTPSTKIFKELFNYDFEKLSQITLHTLQVEK
                     SNLSDEYYINFLIHIAICIKRISIKRYINLSEGFFRELLEHEYFEKSKILCANLSEAF
                     DVEFIDEEVCYILLHIEGLLKSIKKTTTLEPLKNEEITEYNLSEAIAVHWGSILNLNL
                     KDDLILIKSLANHLKSVLHRINYGFTITNPILNDIKRTFPYTYKAAKESNTVIKQLMN
                     YEVNDDEIGYLALYLISAIDRSKSPLNTILICHCSDGVSNLLVQKLSFEFNQINIVKS
                     IPLSSISFTNFDDVDLILTTAPVDFEHNAELININALLSKNDISRLSTIIKKLYSEKN
                     KILSYK"
     gene            complement(5491..6132)
                     /locus_tag="ACGI59_003513"
     CDS             complement(5491..6132)
                     /locus_tag="ACGI59_003513"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HFG9741265.1"
                     /translation="MKYLIDSANLDEIQKCLKYGFKGITANPSMYLKEKVNFHEFIET
                     CANLNPEILTAEVIGNNLYELINSCDKILNINKNTIIKINFSEVGLELINILNSKGVK
                     TACTLIFNVNQASLAINAGADYLFPFIGRNDENGNDGIKNLTEICNLISINGYNTKVV
                     AASIKNVFHLTQASLCGCDYAAVTYDLFKKASFNQLTIDGANTFERDWSKVNK"
     gene            6402..6596
                     /locus_tag="ACGI59_003514"
     CDS             6402..6596
                     /locus_tag="ACGI59_003514"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HFG9741266.1"
                     /translation="MINFYQPSRINFGQGTLSELPRIIGKYGKKCLLVTTPNVEPLDK
                     LYKRVTLNLIEKGIEVVHFD"
BASE COUNT         2559 a          887 c          997 g         2519 t
ORIGIN      
        1 tcaattatta taattatttt atattaaata ttacattaat ctattgttct agttaaaatc
       61 gtatttcaat taaattcaat taaaataaat aactctttat aaaaagcaat tatttaggta
      121 gaggaatatt tgctaacttt aatagtcttt gttaacttta attttattta agttattata
      181 gtcttactaa ttttataatt gtagatttaa ctactttatg ttcttttatt gtttatgtag
      241 ttgatttaga agttatttta accatttatt ttaattgaag ttgtttgact tttaaatcct
      301 ttgtgtaatc tttgtttttc tagccatgat aaaagtattt ctatttgtat cactatatat
      361 aataatatca tgacaaaata tttattgtct aactaattat aatctatcca aaatgaaaat
      421 gggtgtgaga tattattaca acaaattgta ttagtattaa aaagattaaa aaattaaaaa
      481 actaaacagt taaataatta aacgattaag tgactgtcat ataacaattt ataattttat
      541 ttaacagcca cttaggtatt taagagcgat agaattgagt gtatatcaaa ttataataat
      601 ttcctagtaa ataattgaaa aataaggtag tccattttta tatttgatat ctggtttacc
      661 ttgaataagt ggatatagat aatttagggc ttcatcagtt atataattac attcttcatt
      721 tatccactct ttaggaaaat attttgtctt gttagctatt ttagaaatat cagtatgact
      781 aacttcatac ttataaggat tattgcatgt tctattaatg atagccataa ctcctgtata
      841 tcctttttta gatagttcta tcccatatgc accaacttca gctgcttgtt ttaaatcagt
      901 atctgaagct aaatgcatag cacatctttg tattacacca agttctaagg tttttactct
      961 tgttgtaatt gatgatgata ctataagatt ctttagatag ttgcttactc cacctaattg
     1021 ggaatgacca aatttatcat gagcagaagt tttttgttcg catataaagt tgccgtattt
     1081 atcccttata ccttctgatg caactatata agccttattt ttttctttaa atatttttct
     1141 aacatcttct ataaacttat cttcatagaa atcaatttct ggaaggtata ttaaatcaac
     1201 aagtggagta tcattaagtc tagctagtga agaagaagct gctaaccatc cagtatctct
     1261 acccatagtt tctactataa atataccatt atttttgtat atagaaaaat ctaagtatgt
     1321 ttccattatt agtgtagaaa taagctttgc agcagaacca aatccaggac agttgtcagt
     1381 tcctaataaa tcattatcta ttgttttagg aatacctata aattttattt tacaagtagt
     1441 gtttttgcag tattgagaaa atttcatcac tgtgtccata gaatcatttc cgcctatata
     1501 aaataatgta tctattttat attcatctag tatatttata actctatcat aatcataaga
     1561 atttatttta taatcagcca gtttgtatct acaagaacca agaccagacg aaggtgtttg
     1621 ctttaaattt aatacattat ctttgtttaa ggagtctgaa tctattatat ttttgtttaa
     1681 tataccttca atcccattga taccaaaata tactttatca aagtatttag tttctaaatt
     1741 tttttcataa ataccaactg cagaagcatt gataacagct gtagggcctc cagattgggc
     1801 aataatacag tttctcattt tacatctccc tttcattaat attgttatga atggtacttt
     1861 ttatcgagta aatttatatt tatagtctga ttatatatta aatgaactag ctatatcaat
     1921 tgagttagta tagacaataa atgccataag aatgtggtaa aaaactaact ttttatatac
     1981 aattaatgtc tataatttac cacaatataa aggtactttt aaggagtagt caaagtgttg
     2041 tgaaaaaaaa tatatcagtg taatattaat ttacaaagca aaataagttc acagttaaca
     2101 aaagcagaat aagttcataa aagaaatata tacagcaata aaagtaatgc taaataaaag
     2161 taatactgga ttaaaaaata ttaaaaaaat attaaaaact aatatttaat actaattaaa
     2221 gtaaaatatg gaggtataca aatatgaaac acataaaaat agcagcagga ttagcacatg
     2281 taaattacgg tcatatatca gccgtagtta aagaagcaat ggatgcaggt gtagactacg
     2341 tacattcaga tgcagcagat atgcatgact taaaaaatat gcaacttatg ggtggtcatc
     2401 aaataataga agcaataaga ccggtgacag ataaaccaat agaatgccat atatacacaa
     2461 gagattgtga tagattattt atagaaaaaa tagcagcagc aggatgtaac ttgttgataa
     2521 taccagcaga acatttttta ggtgctcctc ttgcatacat aataaattac tgtagagaat
     2581 ttggtatgaa agtaggatta acaattggat gttacacacc actatgtttt gttgatgaag
     2641 ctatatatga tatagataga ttgcaaatag tcgttcatgg tgttgatgaa actgatggaa
     2701 aagataactg gggatggaga aagagtgctg ttgatttagt aaaaagagct agaaaaatga
     2761 tagacgaaaa gaatcctaag tgtgaattag caatagatgg tggactaaga gcagacaata
     2821 tggagccatt aatagaatgt aatccagacg ttgtaatatt atcttcagca atattcaagg
     2881 ataaagatgg tataacagca ggtgttaaga attgtagaaa agctatagat gaagcagcta
     2941 caaagtttgg tttagaataa gataataaac tatgataaaa gagatgcttt cttaatgttt
     3001 taaggaagca ttaattttat gaaaaactac aggaagctag actaatttaa ttgatttttt
     3061 atactgaaac aatataatca taggaggtta ttaaatgaaa aaagaaatga taaaagatta
     3121 tttactgata acaatatcag catttttaat ggcattagca gttaattttt tctttgcaga
     3181 acatacatta gctccaggag ggataacagg attatcagtc gtacttagca cattcttatc
     3241 aatgcctgtt gagaatatat cattaggaat atctatgcca cttttagtaa tgggattatt
     3301 atttttaggg aaaggttttg gaataaaaac attatatata actcttatgg gcccattatt
     3361 cttaaaagta ataccacaaa ctcacataac tgacaatcta ttagtagcag gagtgatagg
     3421 tgggttattg gtaggaactg ctatagggat agcaataata aggggctgta gtactggtgg
     3481 aactgattta gttgcaatgc ttattaataa ggtaattaag tttctgaagt tgccagttat
     3541 attatttatg ctagatggat ttataatagt agcgtctgga ataataagta agaattttat
     3601 gatatctata tattcattga tatctttatt actcataata aaaacaatag gatttgtaac
     3661 tacaaagttt ggtaagaata ctgaagaaat taataatata aataatgctt gtgctcaata
     3721 aaaataataa aatttacttt aaatataaaa gtttaaaaaa tataaaagct taaataaaaa
     3781 gattaaatat aaaaaatgaa ggtgtctcaa aatgaatttt ttagttcatg aggcactttt
     3841 tctatgaaat caaatatatt tttatcattt ggacaatgaa aaataggttt atctctatgt
     3901 tgagacaagc ctatttttgt ttatttataa gataatattt tatttttttc agaatatagt
     3961 ttttttataa ttgtactaag tctagaaata tcattcttac ttaaaagagc atttatattt
     4021 atcagttcag cattatgttc aaagtctaca ggagcagtgg ttagtattaa gtccacatca
     4081 tcaaagttcg taaatgaaat agaagataga ggtatagatt taactatatt aatctgatta
     4141 aattcaaaac ttaacttttg gactaataaa ttagatacac catcactaca gtgacatatt
     4201 aaaatagtat ttaggggaga tttgcttcta tctattgctg atattaaata taaagcaaga
     4261 tatcctattt cgtcatcatt gacttcataa ttcatcaact gtttgataac agtattagac
     4321 tctttagcag ccttataagt gtaaggaaat gttctcttta tatcatttaa tatgggattt
     4381 gtaatagtaa acccatagtt tattctgtgt agaactgatt taaggtgatt agcaagagac
     4441 tttattaata ttaaatcatc ctttaagttt aaatttagta tacttcccca atgaacagct
     4501 attgcttcag atagattata ctctgtaatt tcttcatttt taagaggttc aagagtagta
     4561 gttttcttta ttgatttaag taatccttct atatgaagaa ggatataaca gacctcttca
     4621 tcaataaatt ctacgtcaaa agcttcactt aggttagcac ataaaatttt tgatttttca
     4681 aagtattcat gttctaataa ttctctaaaa aaaccttcag ataagttaat atatctttta
     4741 attgaaattc ttttaataca tatagctata tgtattaaga agtttatata atactcatca
     4801 ctaaggttac ttttttctac ttgtaaggtg tgtagagtga tttgagataa tttctcaaaa
     4861 tcatagttaa atagctcctt aaatattttt gttgatgggg tatctttgca ttcattagat
     4921 ttcgagaata atatttcttt aagttcagta taacttttat taaccgtagc aagttcaaaa
     4981 attgcttttc gaatatcttt ctcttttccc tctaaatata ctccattatt ggtttttcta
     5041 actagagtca ctttaaagtt ttgcaaaaaa ttttgaataa ctaccaagtc tttatgcaca
     5101 gtagctctac taacatgtag ttcattgcaa atatcacgaa tcctaaactt agaattttta
     5161 ataaataatt tacttagaat ataccttttt ctatcttcag gagagtatgc atatatacaa
     5221 tttgatttat cattgatatc tctaataact ttaagtttta tatcttcatt acctagaata
     5281 ataacccctg aaccagtttt tttatctagg gataaattaa actctaaaag ccattcatct
     5341 aaaataacaa ggtcattacg aatagtttta ttactaactt ttaattcttc ggcaatagta
     5401 tctacagtaa tatagtctga gctgttgagt aataattcag caatatctaa taatcttttg
     5461 ttcaatgcaa accctcctaa atcttagtta ctatttatta accttggacc aatctctttc
     5521 aaaagtattg gcaccatcta ttgtaagctg gttaaaagaa gcttttttaa ataaatcata
     5581 tgttacagct gcataatcac atccacaaag agatgcttga gttaagtgga atacattttt
     5641 aatagaagct gcaacaactt tagtattata tccatttatt gatataaggt tacatatttc
     5701 tgtaaggttt tttataccat cattcccatt ttcatcattt ctacctataa aagggaatag
     5761 gtaatcagca cctgcattta tagctaatga tgcttgattt acattgaaaa tcaaggtaca
     5821 tgcagttttt acacctttac tatttaatat gtttataagt tctaagccaa cttcagagaa
     5881 atttattttt attatagtgt ttttatttat atttaatatc ttatcacatg aattaattaa
     5941 ttcgtataaa ttatttccaa tgacttcagc agttaatatt tctggattta aattagcaca
     6001 tgtctcaata aattcatgga agtttacttt ctctttaaga tacatagatg gattagcggt
     6061 tattccctta aatccatact ttagacactt ttgaatttca tcaagattag cactgtctat
     6121 caaatatttc ataatttaat tctcctttaa gctatttgtc ataaaaaagt gtatatactc
     6181 tatttttatt attaagtcaa ttatacaata agattgatta taaaattata acatgggtac
     6241 ttaataaaat ttacataatg atatggtaat aataaaacat tgattgtcta ataattacca
     6301 taagctttag taaataagag ttaacagtga aggtattgcc ttaaaaatcc tatgatatgt
     6361 ataatttgaa tatgaaggca ataatttaag ggaggaaaaa tatgattaac ttttatcaac
     6421 catcaagaat aaattttgga caaggaactt taagtgaatt acctagaata ataggaaaat
     6481 atggaaagaa atgtttgtta gttactactc caaatgtaga gccattagac aagttatata
     6541 aaagagttac attgaattta atagaaaaag gtatagaagt tgtacacttt gattaaaaaa
     6601 tagctacgca cactcgtgac tttagtcatg agttaatagc taaaatagtc agcatatagg
     6661 gaaacctata tgtagagata ggatagaacc tatccaatac tacttgaatt gctggaaacc
     6721 cctaaagcta accaaactaa aacataagga tgaaataaac ctaagtgtga aagttacgaa
     6781 agtagaaaaa attggttaga tggtataagg ttaaatccta agtactttaa aatgggcaat
     6841 cagcaggtaa gctccgaata ggagaaactt caacgactat tcctcttgag ggaagtacac
     6901 tacaagttat cggtagtgga agtgggtaga ccttaacgga taatgccgaa ggataagata
     6961 ta
//
LOCUS       DAYAPA010000168         6712 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.173, whole genome shotgun sequence.
ACCESSION   DAYAPA010000168 DAYAPA010000000
VERSION     DAYAPA010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6712
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.173"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     misc_binding    158..369
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            532..1821
                     /locus_tag="ACGI59_003515"
     CDS             532..1821
                     /locus_tag="ACGI59_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HFG9741267.1"
                     /translation="MEEEKINYALEQIPENQKRGWVAMFSVLVAIGVDLSSVILGAEL
                     AQSMPMKQAILSVIVGSFFSAILYTTCSLVGSSTSLSTSMITKYVFGEAGAKIFSLVI
                     GVSLLGWFGVQVGFFAQNAQIIIKDIFNLDVSMQILSLIGGLLMMSTAIYGYKAMEKL
                     SVYSVPFLLVLMMLTIFLAFRANGISVDDNMKSTMTFAGGVSLSMSIIIVGAIVSPDI
                     SRWAKSRRDCALSSFLGIQFGNAFMIIVSIVLVKCMGTSDIMRIFITLGIAIPGIIVL
                     TLAQWTTNTSNVYSASLSIALVLKKAPEKVLTIVLGIIATLLAVFGIYEGFIGFLNLL
                     GIVIAPVGGVYTAEYYIVKQELKGFDKGVLYKPIVKRSIVSWIIGILITYLSTYGFIT
                     LTTIAPLDGFIAGFVVQSIIGKVLCSTKNKQEIDKAV"
     gene            1875..2954
                     /locus_tag="ACGI59_003516"
     CDS             1875..2954
                     /locus_tag="ACGI59_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF917 domain-containing protein"
                     /protein_id="HFG9741268.1"
                     /translation="MRFLTEESVDKIAVGAAVLGTGGGGDPYVGKLVAKQAIKKYGPV
                     KVISLDELDDDALVVPVSGMGSPVITIEKLLSEVELTTPLEIMEKLLNRKVDVIIPIE
                     IGGINSLMPIAVAAKKGLPILDADSMGRAFPEAQMVTFYLEGYEASPAVMSDEKGNSA
                     ILYPVDGIWSERLARTLTVEMGGSSSISDYNLSGAQVKKAAIGNTLTIAETIGGFLLE
                     NKSDSKGAVENILKELCGYKLFEGKVIDIKRELKGGFTRGHAFFAGINEYDGEYSILF
                     QNENLIAKKGDTPLCITPDLIAVLDLETGFPITTERIKYGSRVMVVAFPCNEKWRTEK
                     GIETVGPGYFGYDVEYKTVEELQGK"
     gene            2975..4519
                     /locus_tag="ACGI59_003517"
     CDS             2975..4519
                     /locus_tag="ACGI59_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase N-terminal
                     domain-containing protein"
                     /protein_id="HFG9741269.1"
                     /translation="MYRVGIDVGGTNTDACILDGELKVIHSVKVATTKDVETGVYNAL
                     KKVIDESKIDHSLIKYAMLGTTHCTNAIVERKRLNNVAMIRIGKPATTAILPFIDWPE
                     DLREKIELDSILVSGGYEFDGRKINELCKDEVVEFCNRIKGKVESVAISGVFAPVNKQ
                     QEEEVAKWVREILGDIPISLSNEIGNIGILERENGAILNSALSQVGKAVSLGFEKALK
                     DLGIDAQIYFSQNDGTLMNLEYTMKYPIFTIGCGITNSIRGASFLSKVKNAIVLDVGG
                     TTSDLGVLYNGFPRESSIPVTIGGVHTNFRMPDVLTIGLAGGTCVKGEKDNIKIGPES
                     VGYRITEDAIIFGGDTLTLSDVVTKLDMAFEDRKSNVENLDKEYCNEVYKKVILKLED
                     AVDQMKTSQDDVELVLTGGGSIIVPEKLKGISNVIRPPHYTAANAIGAALGQISGDVE
                     KVYSLDKMSREDAIEDAKKEAVNKALKAGAKEDTIEVLNIEDVPLAYLPGNALLIKVK
                     VVGDLI"
     gene            complement(4682..5224)
                     /locus_tag="ACGI59_003518"
     CDS             complement(4682..5224)
                     /locus_tag="ACGI59_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HFG9741270.1"
                     /translation="MKSYIALLRGINISGKNKIIMSELKASFVELGYSEVSTYLNSGN
                     VVFLSDVDDSKVISNTIRLMIKKQFELEIPVFIISQEELKDILNNAPNWWGDNNKEIY
                     DNLIFMFPDLSYDEFYDEVGNPKEEYEKVYHYKNAIFWSFSRKDYKKTNWWSKTASSN
                     VSDKITIRTANTVRKIVGMR"
     gene            complement(5372..5656)
                     /locus_tag="ACGI59_003519"
     CDS             complement(5372..5656)
                     /locus_tag="ACGI59_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFG9741271.1"
                     /translation="MAKNTKQTTVHIDETLLKEVKRIGINENKSISQIINESIIDYIL
                     VYRENEDKEKNEHDKNLNETINEFKKEEEKRMQAKSAARAIAEAKKRVLY"
     gene            complement(5719..6483)
                     /locus_tag="ACGI59_003520"
     CDS             complement(5719..6483)
                     /locus_tag="ACGI59_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFG9741272.1"
                     /translation="MNNYLKFATYQLIGFTSSIPRLIKIKKNPDKFSLKEKFEFMQKQ
                     AKKSLDIVNIELNIIGKETLPKEPLLFVVNHSSMLDSFILTASVERPIGCVIADEPVW
                     RNIPIFKEWAKLLRCVYVNRKNNREGIKSIAQASQNILTGQSMAVFPEGDLTWIKEPN
                     SLVSEFRSGALKIAYKAKCPIVPLVIKNSKDTYEGYQPIGKINSVPVEVEFLEPIYDH
                     IENPRLKSSVLGENIKNKMINTIENFRKSNKTFKEF"
BASE COUNT         2354 a          823 c         1142 g         2393 t
ORIGIN      
        1 attttgatga aatttaaaaa aatacattat ataaatatag taatagtatg aaaatttaca
       61 tataaaacta aaaaatgcaa gtaattgact ttttaagttg acaaaatatt gcatatatat
      121 tatgatataa atcataaatt aactacaaat caatattata tattcgtaga agaagaaagt
      181 agcataaagg aattttacag agagttagat ttggctgaga actaatattg gaatttatgt
      241 gaaaaggact ttggagaata ctattgtaat tatgttagaa tagtggttta attcgcctta
      301 agaaaatgag tgagtaaaat taatttttac taataagagt ggtaacacgg atttatattc
      361 gtctcttgga tttttccaag aggcgttttt tattataaac ttatttcaga aaatttaaaa
      421 ctatatatta aatgaaaatt ataaaaatat gatagttaat aaatttataa attagaaaat
      481 tgtaaaatat gctaaggtca gtagttttac gatttaaatg gagggatgaa aatggaagaa
      541 gaaaaaatta actatgctct ggaacagatt ccagagaatc aaaaacgtgg ttgggtagct
      601 atgttttcag ttttagtagc aataggtgtg gatttatcat ctgttatttt aggcgctgag
      661 ttggcacaga gtatgccaat gaaacaagca atattgtcag taattgttgg ttcatttttt
      721 tctgcaattt tgtatactac atgctctttg gttggttcat ctacaagttt atctacatct
      781 atgataacaa agtatgtatt tggagaagct ggagcaaaaa tattttctct agttataggg
      841 gtttctctat taggttggtt tggagttcaa gtaggtttct ttgctcaaaa tgcacagata
      901 ataataaaag atatatttaa tttagatgtc agtatgcaga ttttaagcct aataggtggt
      961 ctcttgatga tgagtactgc aatttatgga tataaggcaa tggagaagtt gagtgtgtat
     1021 tcagtaccat ttttattagt tcttatgatg ctaactatat tcttagcttt tagagcaaat
     1081 ggaatttcag ttgatgataa tatgaaatct actatgacat ttgcaggtgg agtttctctg
     1141 tcaatgagta taataatagt tggtgcaatt gtatctcctg atataagtcg ttgggctaaa
     1201 agtagaagag actgtgctct ttcaagcttt ttgggaatac aatttggaaa tgcttttatg
     1261 ataatagttt cgatagtatt agtaaaatgt atgggaactt cagatattat gagaattttt
     1321 attacattag gaattgctat accaggtata atagtactta cattagcaca atggactaca
     1381 aatacaagta atgtatattc agcatcatta agtatagctc ttgtacttaa gaaggctcct
     1441 gagaaagtat taactatagt ccttggtatt atagcaacat tattggctgt atttggaata
     1501 tatgaaggtt ttataggatt tttaaattta cttggaattg taattgcacc tgttggtggt
     1561 gtatatacag cagaatatta catagtaaag caagaactta aaggctttga taaaggagta
     1621 ttatataaac caatagttaa acgttcaata gtgtcttgga taattggtat attaataact
     1681 tacctttcaa catatggatt tataactctt actactatag cacctttaga tggttttata
     1741 gcagggtttg tagtacaatc tattattgga aaagtgcttt gttcaacaaa aaataagcaa
     1801 gaaatagaca aagcagtata aagaaatttg tttataaagt ataaaataaa ttagaaatat
     1861 gaggagaaag taacatgaga tttttaacag aagaaagtgt agataaaata gcagttggtg
     1921 cagcagtgct tggaactgga ggtggaggtg acccatatgt aggtaaattg gttgcaaaac
     1981 aagccattaa aaagtatggt cctgtaaaag taataagttt agatgaactt gatgatgatg
     2041 ctttggtagt tccagtatct ggtatgggtt ctcctgtaat tacaatagaa aaattacttt
     2101 cagaagtaga gttaactacc cctttagaaa taatggagaa attattaaat agaaaagtgg
     2161 atgttataat tcctatagaa ataggaggga taaactcact tatgcctata gcagttgctg
     2221 ctaaaaaagg acttcctatc ttagatgcag attctatggg tagagctttc cctgaagctc
     2281 aaatggtaac tttttatctt gaaggatatg aggcatctcc tgccgtaatg tctgatgaaa
     2341 aaggtaattc tgccatcctt tatcctgtgg atggaatttg gtcagaaaga ttagctagaa
     2401 ctttaactgt agaaatggga ggaagttctt ctattagtga ctataattta agtggagctc
     2461 aagttaaaaa agctgctata ggtaatactc ttacaatagc tgaaactata ggagggtttt
     2521 tacttgaaaa taaatctgat tcaaaaggtg ctgttgagaa tatactaaaa gaattgtgtg
     2581 gatacaagtt atttgaagga aaagtaattg atattaagag agagttgaaa ggtggattta
     2641 ctagaggaca tgcctttttt gctgggatta atgagtatga tggagagtat tctatattat
     2701 tccaaaatga gaatttaatt gccaaaaaag gtgatacacc actttgtatt acaccagatt
     2761 taatagcagt attagattta gaaactggat ttccaataac tacagagaga ataaaatatg
     2821 gctctcgtgt tatggtagta gcttttcctt gtaatgaaaa atggagaaca gaaaaaggaa
     2881 ttgagacagt tggacctgga tactttggat atgatgtgga atataaaaca gtagaagaat
     2941 tacaaggaaa ataaaagttt aggaggaaat tttgatgtat cgtgtaggaa ttgacgttgg
     3001 aggaacaaat acagatgctt gtatactgga tggggaatta aaagtaattc attcagttaa
     3061 ggtggcaact acaaaagatg ttgaaactgg tgtttacaat gccttaaaaa aagttataga
     3121 tgaaagtaag attgaccatt cattaataaa atatgctatg cttggtacaa ctcattgtac
     3181 aaatgctatt gtagaaagaa aaagacttaa taatgtggct atgataagaa taggaaagcc
     3241 ggccactact gccattcttc catttataga ttggccagaa gacttaagag aaaaaataga
     3301 attagattca atattggtat ctggtggata tgagtttgat ggcagaaaaa taaatgaact
     3361 ttgtaaagat gaagttgtag agttttgcaa tagaataaaa ggcaaagtgg aaagtgttgc
     3421 aataagtgga gtatttgctc cagtaaataa acaacaagaa gaagaagttg caaaatgggt
     3481 tagagaaatt ttaggggata ttcctatatc tttatcaaat gaaataggta atataggtat
     3541 attagaaaga gaaaatggag ctattttaaa ttctgcattg tctcaagtag gtaaagctgt
     3601 atctttagga tttgaaaaag cacttaaaga tttaggtata gatgctcaaa tatatttcag
     3661 tcaaaatgat ggaacattaa tgaatttaga gtatacaatg aaatacccaa tttttacaat
     3721 tggatgtgga ataacaaatt ctataagagg agcttccttc ttatcaaaag taaaaaatgc
     3781 aatagtatta gatgttggtg gaactacaag cgacttgggt gtactatata atggattccc
     3841 tagagaatca tctattccag ttacaattgg aggagtacat acaaacttta gaatgccaga
     3901 tgttttaact attggtcttg ctggtggtac atgtgtaaaa ggcgaaaaag ataatataaa
     3961 gataggtcct gaaagtgtgg gatatagaat aactgaagat gcaataatat ttggtggaga
     4021 cactctaact ttatcagatg ttgttacaaa attagatatg gcgtttgaag atagaaaaag
     4081 caatgttgaa aacttagata aagagtattg taatgaggta tataagaagg ttattttaaa
     4141 attagaagat gctgtagacc aaatgaagac tagtcaagat gatgttgaat tggttttaac
     4201 tggtggagga agtataattg ttcctgaaaa actaaaaggc ataagcaatg ttataagacc
     4261 tcctcattat actgctgcaa atgctattgg tgcagcttta ggacaaataa gtggagatgt
     4321 agaaaaagta tacagtttag ataaaatgtc gagagaagat gccatagaag atgcaaaaaa
     4381 agaggctgta aataaagctc taaaagctgg tgctaaagaa gatactatag aagtattaaa
     4441 tatagaagat gttccacttg cataccttcc tggtaatgca cttcttataa aagtaaaagt
     4501 tgttggtgat ttaatttaaa gaataaatat aagtatttcc agaattatat aaatatttct
     4561 ggactcattt ttaaacttat tttatcaata aaaacagcat aacttttgac tatattctta
     4621 taagtttccg aacagccgat atatacctat tttttgaggt ttatcggctg tttgttatct
     4681 tttatcgcat tccaactatt tttcttaccg tatttgctgt acgaattgta atcttatcac
     4741 ttacatttga actggcagtc tttgaccacc aatttgtctt tttataatct ttacgactaa
     4801 aagaccaaaa gatagcattt ttataatgat atactttttc atactcttct ttaggatttc
     4861 caacctcatc ataaaactcg tcataagata agtcaggaaa cataaaaata agattatcat
     4921 atatttcttt gttgttatct ccccaccaat taggagcatt atttagtata tcttttaact
     4981 cttcttgtga aataataaaa actggtattt ccaattcaaa ctgttttttt atcattaatc
     5041 ttattgtatt tgagataacc ttgctgtcat ctacatcact taaaaagaca acattaccac
     5101 tattaaggta tgtagaaact tctgaataac caagttctac aaagcttgct tttaattcag
     5161 acataattat cttgttctta ccacttatat tgatacctct taataatgcg atataacttt
     5221 tcatataata atttcacctc taaacattca catttttcta tgtagtacaa acttgaattt
     5281 gcatctttta taattctttc aaaacttctt catatgtaat ttttttaata taaaaaaatc
     5341 ggtgcttaat ttgtaccgat ttttgttttt cctagtaaag aactcgtttt tttgcttctg
     5401 ctatagctct agcagcactt tttgcttgca ttcttttttc ttcttctttt ttaaattcat
     5461 ttattgtttc atttagattc ttatcatgtt catttttttc tttatcttcg ttttctctgt
     5521 atactaatat ataatctatt attgattcgt ttattatctg agatatactt ttattttcat
     5581 taattcctat tctcttaact tccttaagta gagtctcatc aatatgaacc gttgtttgct
     5641 ttgtattttt tgccacctta atcacctctt aaaaaaaatt ctaacattaa aatttttttt
     5701 agtcaataaa ttttaatatt aaaattcttt aaatgtttta tttgattttc taaaattttc
     5761 tattgtattt atcatcttat tttttatatt ttcacctagt acagaagact ttagtctagg
     5821 attttcgata tggtcataaa ttggttctaa aaattcaact tcgacaggaa cagagttaat
     5881 tttaccaata ggttggtatc cttcataagt atcttttgaa ttttttatta cgagtggaac
     5941 aatagggcat tttgctttat atgctatctt taaagctcca cttctaaact ccgaaaccaa
     6001 tgaatttggt tctttaatcc atgttaaatc tccttctgga aacactgcca tactttgacc
     6061 tgtaagtata ttttgagagg cttgggctat actctttata ccctcacgat tgttttttct
     6121 gtttacatat acacatctta gtaattttgc ccattcttta aatataggta tatttctcca
     6181 aactggttca tctgcaatta cacatccaat tggtctttct acacttgctg ttagtatgaa
     6241 gctgtcaagc atactagaat gatttacaac aaatagaagt ggttcttttg gcagtgtttc
     6301 cttaccaatt atattaagct ctatattcac tatatctaaa gattttttag cttgtttttg
     6361 cataaattca aatttttcct ttaagctaaa tttatcagga ttctttttaa ttttaataag
     6421 cctaggtata gaagatgtaa aacctataag ttgatatgta gcaaatttta agtaattatt
     6481 cataaaatta tctccttaaa ataaaattat ttattactgt aaagatttta acataaaata
     6541 aaatttaagt ctattttaat tcaattttgt aacatatatt ttaaatagac agagattgag
     6601 attttatatt tccaatttta gtaatcagat tttatcaata aattactatt ctattaattt
     6661 aattaaataa cctttaaatt taattataaa aactgttgca gaaatttcta ca
//
LOCUS       DAYAPA010000169         6582 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.174, whole genome shotgun sequence.
ACCESSION   DAYAPA010000169 DAYAPA010000000
VERSION     DAYAPA010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6582
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.174"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(87..917)
                     /locus_tag="ACGI59_003521"
     CDS             complement(87..917)
                     /locus_tag="ACGI59_003521"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFG9741273.1"
                     /translation="MLNKLCMYLERPELYKQSEINFWDDEYISEQLLKAHLDTNFEGA
                     SRNFNFIEDSVNWIVTVANPVNYPKLLDLGCGPGLYAEKFAQKGYKVTGIDFSKRSIN
                     YAQNRNKETNLNINYLFQSYLNMNYNEEFDLATLIYCDYGALSTENRRLLMEKIYDSL
                     KPGGKLILDVFTINKYNNFEEIKYWEINEDGGFWSNEKYMCLQDNCKYNDYNTLEQTL
                     VITEKDENVFYVWNHYFSKESFLLEVENIGFKSVEFFSNVKGEAYSDDSMTMGLVLQK
                     "
     gene            complement(1269..2171)
                     /locus_tag="ACGI59_003522"
     CDS             complement(1269..2171)
                     /locus_tag="ACGI59_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFG9741274.1"
                     /translation="MYNKIKYIPINCEIACNKLKRSMPYKWDLNIYRGCEHGCKYCYA
                     IYSHKYINSNNYFEEIYVKTNIVEKLERQLKSSKWKKEVINIGGVTDSYQPIEADYKI
                     MPEILNLLIKYKTPAIISTKSDLILRDYDLIDKLSRITYINVASTITTVNEDTQRLIE
                     PNGVDSMRRFKMLKEFRKTNASVGLHIMPIIPYITDDFDNINSLFRHAKECNVHYVLP
                     GTLYLRGITRGVFFDFVKKEFPELFDKLSILYSEGSLNREYKNQLYKMVNELRYKYSL
                     SSSYSKIMKEKLKNSGDVQISFFD"
     gene            complement(2221..2682)
                     /locus_tag="ACGI59_003523"
     CDS             complement(2221..2682)
                     /locus_tag="ACGI59_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine dimer DNA glycosylase/endonuclease V"
                     /protein_id="HFG9741275.1"
                     /translation="MRLWHKDLIDVLPKNQLVSQWRELLAIKGSIDKKGTPNHLLVNK
                     VLNYSIDEFKFYTKIVHDEMLKRNYKPNELKYTSILKWKNRNFANDISNEHSLNLENL
                     YDDWHNKMYLKQCLYNLEEKATCGGIPINEWNILLCKYSKDYELWSGNIMF"
     gene            complement(2734..3189)
                     /gene="def"
                     /locus_tag="ACGI59_003524"
     CDS             complement(2734..3189)
                     /gene="def"
                     /locus_tag="ACGI59_003524"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFG9741276.1"
                     /translation="MAIRKIRTFDDEILRKKSKYVENVDNKIREILNDMAETMYNTPN
                     GGGLAACQVGVLKRLVVIDLGEGLIKLVNPEIIKQEGEQIVVEGCLSFPEVWGKLKRP
                     KKVTVQALNEYGEKIEIKGSGFMAKCLCHEIDHLNGIVFTDKIIEHVKL"
     gene            complement(3161..3424)
                     /locus_tag="ACGI59_003525"
     CDS             complement(3161..3424)
                     /locus_tag="ACGI59_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFG9741277.1"
                     /translation="MKNKIKILREKLGLTQEQLGRLVGTSRQAINAIETGKNEPSIWL
                     AYDISRIFNEPIESVFLFEESERKSRAQISRGEYYGYKKDKNI"
     gene            complement(3521..4237)
                     /locus_tag="ACGI59_003526"
     CDS             complement(3521..4237)
                     /locus_tag="ACGI59_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9741278.1"
                     /translation="MIKIINAFKQEFEQIKRDAMLFIVCVSPILCGVFIKFGIPLIQN
                     ISLNKFYYQLNLEPYFLMFDLLLAFITPFMFFFASTMVILGEIDDSISRYLIVTPLGK
                     TGYLISRFGIPGILAFIITMILLIFFSLTKISFLLNLSISLLVLLQGIIISILVISLS
                     SNKLEGMVITKFSGVFMMGILAPFFILNKAQYILFFLPTFWISKAFKEDNYVYMFISI
                     VISLIWILLLFKKFSKKIVK"
     gene            complement(4234..4917)
                     /locus_tag="ACGI59_003527"
     CDS             complement(4234..4917)
                     /locus_tag="ACGI59_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9741279.1"
                     /translation="MRLKNLILGDIKFQFKYGFYFLYLFLSIIYICIINVFPTFMREK
                     IAIIMIYSDPAAMGLFFMGAIVLLEKSQRVLNSLAVSPVKVSEYILSKVISLGVISSI
                     VAMFIAITLNLDNIIISTIGTFFSSIIFSLLGLILASKASSLNQFIVLSIPIEIICFI
                     PPILNVLLDTKSYANLYPFNICISLISGDKNFIMINILILISIIIIIYFITYYFICSS
                     WKKVGGVKL"
     gene            complement(4914..5759)
                     /locus_tag="ACGI59_003528"
     CDS             complement(4914..5759)
                     /locus_tag="ACGI59_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9741280.1"
                     /translation="MIKVDDLSFSYTERDFLQNINFGVGKGEILGFLGPSGAGKSTLQ
                     KILIGMITNYGGSVIVNGVESKRHSNKFYENIGVDFEFPSLYEKLTAIENLKYFGSLY
                     SKKLLSIDELLKSVGLENEANKRVSEYSKGMKSRLNFIKALLHNPDILFLDEPTSGLD
                     PSNSKVMKDIILSEKSKGKTIILTTHNMLDATELCDRVAFIVNGKISALDTPHNLIMS
                     KGAIKVRYTYFDNGEKTSECFLNNTANDKNLNMLIEKNKLLSIHSSEPTLNDIFIEIT
                     GRNLQ"
     gene            complement(5873..6463)
                     /locus_tag="ACGI59_003529"
     CDS             complement(5873..6463)
                     /locus_tag="ACGI59_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFG9741281.1"
                     /translation="MPKSYSDKEREYIIKRLKEEARLCMEQYGIRKTTVDELVKRVKI
                     PKGTFYLFFQSKELLFFEVLRDIHDSIQKEILYEINRVDESITCEQLTDIFMKFYRMV
                     DSTSILNLMINGEFEILVRKLPDSIIEEHFRHDDFEIGEIISCIPNAKNKDIESFSGA
                     FRAVFLTMLYKREVGSNCFEDALRLMINGLVIQLME"
BASE COUNT         2321 a         1051 c          611 g         2599 t
ORIGIN      
        1 tttccatttt tcaatatgta agcttttctt atgttatttt attgttaaag gtttcaagtt
       61 tttatttatt cattttaaaa aaattattat ttttgtaaaa ctaatcccat agtcatacta
      121 tcgtcagaat atgcttcacc cttaacatta ctaaaaaact caacagactt aaatccaata
      181 ttttcaactt ctaataagaa actttctttt gaaaaatagt gattccaaac ataaaataca
      241 ttttcatctt tttcggttat tacaagagtt tgttctaatg tattataatc attgtattta
      301 caattatctt gtagacacat atatttttca ttactccaaa atcctccatc ttcatttatt
      361 tcccaatatt taatttcctc aaaattattg tatttattta ttgtaaatac atctaatatt
      421 aattttcctc caggttttaa actatcataa attttttcca ttaataacct tctattttca
      481 gttgacaaag ctccataatc acaataaatt aaggtagcca aatcaaattc ctcattatag
      541 ttcatattta aataactttg aaacaagtaa tttatattta aattagtctc cttatttcta
      601 ttttgtgcat agttaataga acgttttgag aaatcaattc cagttacctt ataacctttt
      661 tgtgcaaatt tctctgcata taaacctgga ccacaaccta aatcaagtaa tttaggataa
      721 ttaactggat ttgcaactgt aactatccaa tttacagaat cctcgataaa gttaaaattt
      781 ctacttgctc cttcaaaatt agtgtctaag tgtgctttta ataattgctc tgaaatatac
      841 tcatcatccc aaaaattaat ttcactttgt ttataaagct ctggtctttc taaatacata
      901 cataatttgt ttaacaaaac ataccactcc ttaaaattta tttttaaaat aaaaaaacca
      961 cagagtttac tctgtggttt aacttcaaaa agatgagttt aaataagagt aaaatagaac
     1021 caaatatttt taagcacaac aataaaacta tagctttact ttgtttgctt attaaatttg
     1081 attttattca ctcttttcca gcaatactta tatcaacgat tttatgatat caaatatcac
     1141 cgaacaacat catcctttca tatttttatt ttatgaatta attatatatt taaattttat
     1201 atttgtcaat aaattttaat tttaataaat tttaatcatc atagtaaaaa aaattttttt
     1261 atataaaact aatcaaaaaa agatatctgt acatctcctg agttttttaa cttttccttc
     1321 attattttag agtaactgct tgatagagaa tatttatatc taagttcatt aaccatttta
     1381 tacaattgat ttttatactc cctattaagt gaaccttcac tatatagtat tgataattta
     1441 tcaaataatt caggaaactc ttttttaaca aagtcaaaaa aaacacctct agttataccc
     1501 cttaagtata aagtcccagg taaaacataa tgtacattac attcttttgc atgtctaaat
     1561 agcgaattta tattatcaaa atcatctgta atatatggta taattggcat aatatgtaga
     1621 ccaactgatg cgttagtttt tctaaattct tttaacattt taaatcgtct cattgaatcc
     1681 acaccatttg gctcaataag cctttgtgta tcttcattta ctgtggtaat tgtagatgct
     1741 acattgatat atgtaatcct agatagctta tcaattaaat cgtaatctct taaaattaaa
     1801 tctgactttg tagaaataat agctggtgtc ttgtatttta ttagcaggtt taatatttct
     1861 ggcataattt tatagtcagc ttctattggt tgataactat ctgtaactcc tcctatattg
     1921 attacttctt tcttccattt actgctttta agttgtctct caagtttttc tactatattt
     1981 gtttttacat aaatctcttc aaaataatta ttggagttga tgtacttatg agagtaaata
     2041 gcataacaat atttacatcc atgctcacat ccacgataaa tatttaaatc ccatttatat
     2101 ggcatactcc tttttaattt attacaagct atttcacaat ttattggaat atattttatt
     2161 ttattataca aataatcacc tctttatata caataatact ataatgttaa tctatagtaa
     2221 ttaaaacatg atatttccag accacaattc ataatcttta ctatatttac aaagtaatat
     2281 attccattca tttattggta ttcctccaca tgttgctttt tcttctaaat tatataagca
     2341 ttgctttaaa tacattttat tatgccaatc atcatataaa ttttctaaat ttaaactatg
     2401 ttcattagat atatcatttg caaaatttct atttttccat tttaatatac ttgtatattt
     2461 aagttcatta ggtttataat ttctttttaa catttcatca tgtacaattt ttgtataaaa
     2521 tttaaattca tctatactat aattcaatac tttattcact aatagatggt ttggagtacc
     2581 ttttttatct atagaacctt ttatagcaag tagctctctc cattgactta ctaattgatt
     2641 tttaggtaat acatcaatta aatccttatg ccataatctc ataatttaac tccttactat
     2701 aaaatatttt ataaattcaa taaccaatct atcttacaat tttacatgtt caataatttt
     2761 atctgtaaaa actataccat ttaaatggtc tatctcatga caaagacatt ttgccataaa
     2821 acctgaacct ttaatttcta ttttctcacc gtattcgttt agagcttgca ctgtaacttt
     2881 ttttggtctt tttaatttac cccatacttc aggaaaactt aaacatcctt caacaacaat
     2941 ttgttcccct tcttgtttaa taatttcagg attaacaagc ttaataagtc cttctcctaa
     3001 atctataaca actaatcgct ttaatactcc tacttggcat gcagctaatc ctcctccatt
     3061 tggagtattg tacatggtct ctgccatgtc atttaatatt tctcttatct tattatctac
     3121 gttttctaca tacttactct tttttcttaa aatttcatca tcaaatgttc ttatctttct
     3181 tatagccata atattctccc ctacttatct gtgctcttga ttttctttca ctttcttcaa
     3241 ataaaaatac actttcaatt ggttcattaa aaattcttga aatatcatat gccaaccata
     3301 ttgaaggttc atttttacca gtttctatag catttatagc ctgtctagat gttccaacta
     3361 atctccctag ttgttcttgt gtcaatccta atttttctct taatatcttt attttatttt
     3421 tcatatttgc tccattcatg taaggttaac tttacatctt aaatgtaaca tttttcaaca
     3481 ttactgtcaa gtcaacctta catttatata ttaaattttc ttatttaact atttttttac
     3541 taaacttttt aaataataat aaaatccata ttaatgatat aacaattgaa ataaacatat
     3601 agacataatt atcttcctta aatgccttcg atatccaaaa agttggtaag aagaacagaa
     3661 tatattgtgc tttatttaaa ataaaaaatg gtgctaaaat acccatcata aaaacacctg
     3721 agaatttcgt aattaccatt ccttctagtt tatttgatga taaagaaatc actaatattg
     3781 atataataat tccttgtaat aaaactaata aagaaattga taagtttaac aagaaagaaa
     3841 ttttagttag agaaaaaaag ataagtagaa tcatagtaat aataaatgct aagatacctg
     3901 gtattccaaa tcgtgaaata agataaccag ttttaccaag cggagttact attaaatatc
     3961 ttgaaatgct gtcatcaatt tctcccaata tgaccattgt agaagcaaaa aagaacataa
     4021 aaggtgtaat aaaagctaaa agtaaatcaa acatcaagaa atacggctct aaatttaatt
     4081 gataataaaa cttgttaagt gatatattct gtattaatgg tattccaaat tttataaata
     4141 ctccacataa tattggagaa acacatacta taaaaagcat agcatctctc ttaatttgtt
     4201 caaactcttg tttaaacgca tttataatct ttatcatagt tttactccac ctaccttttt
     4261 ccaactgcta caaataaaat aataagttat aaaataaatt attattataa ttgatattaa
     4321 aattaaaata ttaatcatta taaaattttt atctccacta ataagagaaa tacatatatt
     4381 aaaagggtac aaatttgcat atgattttgt gtcaagaagt acattgagta ttggtggaat
     4441 aaagcatata atctcaatag gaatacttaa aacaataaac tgattcaaac tagatgcttt
     4501 acttgcaagt atcaatccaa gcaaagaaaa tataatagaa gaaaaaaagg tacctatagt
     4561 tgatattata atattatcta aatttaatgt aatagcaata aacatagcta caatactaga
     4621 tattactcct aatgaaatta cctttgataa aatatactca gatactttta ctggagatac
     4681 agctaaggaa tttaaaaccc tttgactttt ctcaagaagt acaatagctc ccataaaaaa
     4741 caatcccatt gctgctgggt cagaataaat cataattata gctatttttt ctctcataaa
     4801 agttggaaat acattgataa tacaaatata tataatactt aaaaaaagat ataaaaaata
     4861 aaaaccatac ttaaattgaa actttatatc tcctaaaata agatttttta gcctcattgt
     4921 aaattcctcc ctgttatctc aataaaaata tcatttaatg ttggttcact actatgtatt
     4981 gataaaagtt tatttttctc aataagcata ttcaaatttt tatcattagc agtattgttc
     5041 agaaaacatt cagaagtttt ttctccatta tcaaagtaag tatatctaac tttaatagct
     5101 ccttttgaca taataagatt atgtggtgta tctaaagcag atattttacc atttacaatg
     5161 aacgctactc tgtcacaaag ctctgttgca tctaacatgt tatgagttgt taaaataata
     5221 gttttacctt tagatttttc tgataaaatt atatctttca taaccttgct atttgatggg
     5281 tcaagaccac ttgttggttc atctaaaaat aaaatatctg gattatgtaa taaagctttt
     5341 ataaaattta atcgagattt catacctttt gaatactcag ataccctttt atttgcttca
     5401 ttttctagtc ctactgattt taataactca tcaatagata gtagtttttt agaatataga
     5461 gacccaaaat attttaaatt ttctattgca gtcaactttt catataaact aggaaattca
     5521 aaatcaactc ctatattttc ataaaattta tttgagtgtc ttttactctc tactccattt
     5581 acaatgacac ttcctccata attagttatc attccaatta gaatcttttg taatgtagat
     5641 tttcctgcac ctgatggtcc taaaaatcct agtatttccc cttttcctac tccaaagtta
     5701 atattttgta aaaaatctct ctctgtataa ctaaatgata aatcatcaac tttaatcatt
     5761 caatatcacc ctttcaaatt tttgactatt ttgactatat ggtcaataaa atataatttt
     5821 tttgtttgtt aataattttt aataaaagtt attagcaaac aaaatttaaa ttctattcca
     5881 taagttgtat aactaatccg tttatcatta atcttaaagc atcttcaaaa caattacttc
     5941 caacctcgcg cttatatagc atggtaagaa aaactgctct aaatgctcca ctaaagcttt
     6001 ctatgtcttt attttttgca tttggtatgc aagatataat ctctccaatt tcaaagtcat
     6061 catgtctaaa gtgttcttca attatagaat ctggtaactt tctcaccaat atctcaaatt
     6121 ctccatttat cataagattt aaaattgaag tactatcaac catcctatag aatttcataa
     6181 atatatctgt caattgttca catgtaatac tttcatctac tctatttatc tcatataaga
     6241 tttctttttg tatagagtca tgtatatctc ttaatacctc aaaaaagagc aattcttttg
     6301 attgaaaaaa tagataaaaa gttccttttg gtatttttac ccgttttaca agctcatcaa
     6361 cagtagtttt tctaatacca tattgctcca tacacaatct tgcttcttct ttaagtcttt
     6421 ttattatata ctctctttct ttatcagaat acgatttagg catcttttcc ctccagtttg
     6481 actaaaataa tttatatagt caatataata ctttttctat ctaatgtcaa tagtattatt
     6541 agtatattta aaaataagct taaaaacttt cgaaaattct ga
//
LOCUS       DAYAPA010000170         6479 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.175, whole genome shotgun sequence.
ACCESSION   DAYAPA010000170 DAYAPA010000000
VERSION     DAYAPA010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6479
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.175"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(370..777)
                     /locus_tag="ACGI59_003530"
     CDS             complement(370..777)
                     /locus_tag="ACGI59_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HFG9741282.1"
                     /translation="MSKVAKATFYLMIVTIISKILGMGRELVLSSIYGTGLYTESYLT
                     AMNIPNIIFAAIGTAIVTTFIPMYQDISSKQGEKQALKFLNNVLNIIVGICIVVAILG
                     VIFSKQLVSIFAIGFEGERFLLTVKFTKILIKK"
     gene            complement(1251..2135)
                     /locus_tag="ACGI59_003531"
     CDS             complement(1251..2135)
                     /locus_tag="ACGI59_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFG9741283.1"
                     /translation="MDTKKENNYENYICVGSSNVSNAMKMYLKEIEEYKMLSAGEEVE
                     LAKEIINSSSVAKEKFINSNYRLVVSIAKRYKRDSIDMLDLIQAGNIGLIKAVEKYDY
                     KKGYKFSTYATWWIKQSITRYIDDCENTIRIPIHLHQRINFVKKKKQELLNVLLREPT
                     IDEIADACGLEVDKVLELLRRDKNVVSLDTPLKEDEDSSLVEFIPSDADFKDVVIHEV
                     EQHNLKEKIEELLTGLGEQEQQVLRMRFGIDDDDPKTLEQIGKVFGVTRERIRQIEAK
                     AIRKLRHPSKLKQLKHFY"
     gene            complement(2426..2902)
                     /locus_tag="ACGI59_003532"
     CDS             complement(2426..2902)
                     /locus_tag="ACGI59_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HFG9741284.1"
                     /translation="MSKELVKDRVIKYLIEDLLVPQDMIDTNVELAEFEEGAEGILDI
                     VVNVKDEEDYYAPVMIVQCLDEDVELEGEVLQKQIEFLEEVDNITMSGRLVLTNGDAM
                     MYADWRGEEYDTEAALPTYDIMVKEFHEMEQQVKDLEEHHHHDENCGCGCNHHHEN"
     gene            3168..4343
                     /locus_tag="ACGI59_003533"
     CDS             3168..4343
                     /locus_tag="ACGI59_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HFG9741285.1"
                     /translation="MKFKKQISSIVVSTMLVLGSMNLSYADGTNVVTLGANLSESQKQ
                     QMLNYFGVKKDQVLVLDVTNAEERQYLQGVATEGQLGKVTISCSYVEPTKKGNGINVK
                     TANLTWVTSSMIASTLTTAGLEDANVIAAAPYPVSGTGALTGIMKAFEDASGKKLDET
                     KKEIASEELVVTGDLGDDIGQEKATGVMNDIKTEIVKNGTKDTNQIANTINNVVNNYN
                     ITITPEQKEQIKGVMKKIADQNYDYNSMKNTLDNVSDNVNEQLKKLGESVKGSGILDT
                     IGGWFSGIGDWFSGLFSGGDKKDLGILNNTNDESLGSDAVINSTEGVQINPQNNTNSN
                     NDSDTNSSKNDTSSNNENNDSSQTNENNTTNNSENQSNNNDSVGFFEKIKNWLSGLF"
     gene            complement(4424..5227)
                     /gene="proC"
                     /locus_tag="ACGI59_003534"
     CDS             complement(4424..5227)
                     /gene="proC"
                     /locus_tag="ACGI59_003534"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFG9741286.1"
                     /translation="MKKIGFIGAGNMASAMIGGIVNSKLVEPNMVIASAYSQGTLDRI
                     DANFGINTTKDSKEVTRTSDIVIVAVKPDIYDDILEEIKDFIDDNKIIVTIAAGKSIK
                     DIESIIGEDKKIVRTMPNTPALVNEAMSSLSINKNINKEDLEAVTEVFNSFGNTEVVP
                     EYLIEAVIGASGSAPAYVFLFIEAIADAAVIAGMPRPQAYKFASQAVMGSAKMVLETG
                     KHPGELKDMVCSPGGTTIEAVKVLEEEGFRASVIKAICACIEKSKKMSE"
     gene            complement(5267..5857)
                     /locus_tag="ACGI59_003535"
     CDS             complement(5267..5857)
                     /locus_tag="ACGI59_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFG9741287.1"
                     /translation="MVLNKDIGAKIKQLRTQKQMTLKDMSEKTNLSIGFLSQLERGLT
                     SVATDSLGKIASVLDVELTYFFMKPKEHKRAVLRSYEKEVFDVENSTFIHYHLSSSLK
                     EKTMLPRLIEILPSKSSEEICCYVHEGEEFVYVLEGTLTVFLGDEQIEMYPGDTIHYN
                     SEKNNHNWVNYTNKVVKILVVSIPNPFEKSDAVKEA"
BASE COUNT         2137 a         1101 c          723 g         2518 t
ORIGIN      
        1 aagcacagac tatatcttat ccttcagcat tatccgttaa ggtctaccca cttccactac
       61 caatagcttg tagtgtactt ccctcaagag gaatagtcgt tgaagtttct cctattcgga
      121 gtttacctgc tgattgccca ttttaaagta cttaggattt aaccttatac catctaacca
      181 attttttcta ctttcgtaac tttcacactt aggtttattt catccttatg ttttagtttg
      241 attagcttta ggggtttcca gcaattcaag tagtattgga taggttctat cctatctcta
      301 catataggtt tccctatatg ctgactattt tagctactaa ctcatgacta aagtcacgag
      361 tgtgcgtagc tattttttaa tcaatatttt agtaaatttt acggtaagta aaaacctctc
      421 tccttcaaat cctatagcaa atatacttac aagttgcttt gagaaaataa ctcctaatat
      481 agctacaact atgcatatac caactattat atttaaaaca ttgtttaaaa actttagtgc
      541 ttgcttttca ccttgtttac tacttatatc ttgatacatt ggaataaaag tagtaactat
      601 agcagtacct attgctgcaa atataatatt tggtatattc atagctgtta aataactctc
      661 tgtatataag cctgtaccat aaattgatga taatactaat tctctaccca ttccaagaat
      721 cttagagatt atagtaacta tcatcaaata aaaagttgct ttagctacct tgctcatttt
      781 atcactccac ttctaacata cctcttttta atcaagtaaa atttagctca ttagatataa
      841 ttctatcaac taatttgcct tgcttagccc tatcaaaata taattaacac ctatttctta
      901 atatatcaca aactacatta ttcttcatat ttttttattt ctccaataag ttttacattt
      961 ttcctatata aattgtatag cttcaattat attcaaagaa atggagttgc tacccgataa
     1021 ctttaaaagt aaaaaacaga ttttattatc tttttaaact atattctaac atattttgtc
     1081 catattgaga aattttacta aactattatg tactttatta aataaataaa ataacagcac
     1141 acctataaaa tacaagtgta ctgttcataa acagatttta taaaaaaata attcctaagt
     1201 agagattaac atgctttctc atactctcat atataatgtg tctaaaaata ttaataaaaa
     1261 tgctttaatt gttttaactt acttggatgt cttaattttc ttattgcttt agcttctatc
     1321 tgtcttattc gttctctggt aactccaaat acctttccaa tctgttctag tgttttaggg
     1381 tcatcatcat caattccaaa tctcattctt aaaacttgct gttcttgctc tccaagacct
     1441 gtaagtagtt cttctatctt ttcttttaaa ttatgctgtt caacttcgtg tattacaaca
     1501 tctttaaaat cagcatctga tggtataaat tcaactaaac tactatcctc atcttctttt
     1561 aatggagtat ccaaagatac aacattttta tctcttctta ataattccaa cactttgtca
     1621 acttctaagc cacatgcatc tgcaatctca tctatagttg gctctcttaa taatacattt
     1681 aaaagttctt gtttcttttt ctttacaaaa tttattctct gatgaagatg tataggtatc
     1741 ctaatagtat tttcgcaatc gtcgatatat cttgttatac tttgttttat ccaccaagtt
     1801 gcataagtgc taaatttgta tcctttttta taatcgtact tttctacagc ttttataagt
     1861 cctatatttc ctgcttgaat taaatccaac atatcaatac tatctctttt atatctcttt
     1921 gctatgctca ctaccaacct ataatttgaa tttataaatt tttcttttgc tactgaagat
     1981 gagttgatta tttccttagc cagctctacc tcttctcctg ctgacaacat tttgtattcc
     2041 tctatttcct ttaaatacat tttcatcgca ttcgatacat ttgatgaacc cacacaaata
     2101 taattttcat aattattttc ttttttagta tccataatca aaccccttct attcatagaa
     2161 tttgctaaaa caaaaataaa taatttaaac ctacataatc tacaattaca taatatccca
     2221 ttctttaaat gaattgtttt gtatattatg tatacaaatt tataaaaact atacctataa
     2281 tattattcta cttctaacag caatatcctc cttatatata taatttattt tattatatgt
     2341 tgttatattc ttagtttttt gaatgatata tgtctaaaaa aagactgttt actaaacagt
     2401 ctttttttat atatatgctc aatatttaat tttcatgatg atgattacat ccacatccac
     2461 aattttcatc atggtgatga tgttcttcta aatcttttac ctgttgttcc atttcatgaa
     2521 actcttttac cattatatca taagttggaa gagctgcctc agtatcgtat tcttcccctc
     2581 tccaatcagc gtacatcata gcatctccat tagttaaaac caatctacct gacatagtta
     2641 tattatccac ttcttctaaa aattctattt gtttttgtaa aacttctcct tctaactcca
     2701 catcttcatc taaacattgc actatcatta caggtgcata ataatcttct tcatctttaa
     2761 catttacaac aatatctaaa attccttctg caccttcttc aaattctgcc aactcaacat
     2821 ttgtatcaat catatcttga ggtactaata aatcttctat taaatatttt attactctat
     2881 cttttactaa ttcttttgac atttattttt ctccttccga ttctataaca ttatcataat
     2941 catttctata ttatagtata ctacattaat tgttttttca atctcttaca aaaattattt
     3001 tttattatct tccttatatt ccctttttta ttacaaagtt gtaatcgcat tgatttaaaa
     3061 aatagtttat atttagtata atagaaatgt agcacatatt tttattcaat atattaataa
     3121 aaaaatatat tgaaaatgta atgtatgtta aatctaggag gaatattatg aagtttaaaa
     3181 aacaaattag ttctattgtt gtgtctacta tgcttgtact tgggagtatg aacctttctt
     3241 atgcagacgg tacaaatgta gttacacttg gagctaactt atcagagtct caaaagcagc
     3301 aaatgcttaa ttactttgga gtaaaaaaag accaagtctt agttttagat gtaacaaatg
     3361 cagaagaaag acagtattta caaggtgttg caacagaggg tcaacttgga aaagttacta
     3421 tctcttgctc atacgttgaa cctactaaaa aaggaaatgg aattaatgtt aaaacagcaa
     3481 acttaacttg ggtaactagt tcaatgattg cttcaaccct tacaacagct ggtcttgaag
     3541 atgcaaatgt tattgctgct gctccatatc ctgtaagtgg tactggagca ttgacaggaa
     3601 tcatgaaagc atttgaggat gcttcaggaa agaaacttga tgaaactaag aaagaaattg
     3661 cttctgaaga attggtagtt acaggtgact taggagatga tatagggcaa gaaaaagcaa
     3721 caggtgtaat gaatgatata aaaacagaaa ttgtaaagaa tggtacaaaa gataccaatc
     3781 aaatagcaaa tacaataaat aatgttgtaa ataattacaa tattactatt acaccagaac
     3841 aaaaagaaca aattaaaggt gtaatgaaaa aaatagctga ccaaaattac gattacaata
     3901 gcatgaaaaa tacactagat aatgtatcag ataatgtaaa tgaacaatta aagaaattag
     3961 gtgaaagtgt taagggttct ggtatactag atactattgg tggatggttt agtggaatag
     4021 gagactggtt ctctggttta ttctcaggcg gagataaaaa agatttggga atcttaaata
     4081 atactaatga tgagtctctt ggctctgatg ctgtaataaa ttctacagaa ggtgttcaaa
     4141 taaatccaca aaataatact aattctaaca atgatagtga tacaaatagc tcaaaaaatg
     4201 atacttcaag taataatgaa aataacgata gttctcaaac aaatgaaaac aacacaacaa
     4261 ataattcaga aaatcaatca aataataatg actcagttgg tttctttgaa aaaataaaaa
     4321 attggctttc aggcttattt tagaaattaa acaaataaaa agaattgcct aaaatggaaa
     4381 ttttctataa aaggcaattc tctttattta caataaattt ttattactca ctcatttttt
     4441 tagatttttc tatacatgca catattgctt ttatcacaga agctctaaaa ccttcttctt
     4501 caagtacttt aactgcttct atagtagttc caccaggtga acataccata tcttttaatt
     4561 caccaggatg ttttcctgtc tcaagtacca tttttgcaga acccataaca gcctgtgatg
     4621 caaacttata tgcttgtggt cttggcattc ctgcaattac tgctgcatct gctatagctt
     4681 ctataaataa aaatacatat gctggtgcag aaccacttgc accaattaca gcttctataa
     4741 gatactctgg tactacctca gtatttccaa aagaattaaa tacttctgta actgcttcta
     4801 agtcttcttt gtttatattt ttatttatcg aaagagaaga catggcttca tttacaagag
     4861 ctggagtatt tggcatagtt ctaactattt ttttatcttc tcctataata gactctatat
     4921 cttttataga ttttcctgct gctattgtaa ctataatttt attgtcatct ataaagtctt
     4981 ttatctcttc caaaatatca tcatatatat ctggtttaac agctacaatt actatatcag
     5041 aggttctagt tacttccttc gagtcttttg tagtatttat tccaaaattt gcatctattc
     5101 tatcgagagt tccttgacta tatgctgatg ctataaccat attaggttca actaattttg
     5161 aatttactat tccccctatc atagcgcttg ccatatttcc tgcacctatg aatccaattt
     5221 ttttcacttt agtacctcct tattgactaa tagtaatcgt taaaatttaa gcttctttaa
     5281 ctgcatcaga tttttcaaat ggatttggta tacttactac caaaattttc actaccttat
     5341 tagtatagtt gacccaatta tgattgttct tttcactatt atagtgtatt gtgtctcctg
     5401 gatacatttc tatttgttca tctcccaaaa acactgtaag agtaccctct aatacataca
     5461 caaattcctc accttcatgt acgtaacaac aaatttcctc actactctta cttggaagaa
     5521 tttcaattaa tcttggtagc attgtttttt cctttaaact agatgataaa tgataatgta
     5581 taaatgttga gttttctaca tcaaatactt ctttttcata acttcttaat acagctcttt
     5641 tatgctcttt tggcttcata aaaaagtaag ttaattctac atctagtaca cttgctattt
     5701 ttcctaagga atctgttgcc acacttgtta atcctctttc aagttgtgat aaaaaaccaa
     5761 ttgacaagtt tgttttttca ctcatatcct tcagcgtcat ttgcttttga gttctgagtt
     5821 gctttatttt tgctcctatg tccttattca aaaccatcac ccctaaataa ttttaatttt
     5881 ttcataatat aaatacaaaa atataaatat aggtactgaa cccatcttta tttcatttaa
     5941 aaaaattttt ataagtatta ctataattta tcaatctttt tttgtctaca tttttacgat
     6001 tatttacgat ttatttttca catatgtata tttaatcttg ccaactaaag atacattttt
     6061 tataagcatt tactctttat ataataaaac ttaagttgtt catgtagtct tcttctggtt
     6121 taattattaa aatttcaaat tatattatta tttctacaca ttattacatc ctttagtaca
     6181 attataacat aattttacaa tttaatgata tctattcaat gtcttttttt actctacaac
     6241 atattttatt ttagagttta atactatata tattttttat atacagcctt gtcatttttt
     6301 agattgtcaa gagttattga agtattttat gtagatttag attttatttc ctaatttctc
     6361 atttaacaag aattgcttat gataaaaaat aatatcatct caataagatt cttgtaaata
     6421 tatctatatt ctaaatataa tttctttttt tgtcaaaatt tcttcattct ttaataatt
//
LOCUS       DAYAPA010000171         6280 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.176, whole genome shotgun sequence.
ACCESSION   DAYAPA010000171 DAYAPA010000000
VERSION     DAYAPA010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6280
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.176"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            202..747
                     /locus_tag="ACGI59_003536"
     CDS             202..747
                     /locus_tag="ACGI59_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFG9741288.1"
                     /translation="MENVITGLSKGAEWFIGLFQKGGEQFIGLVSGTLPTLIVLMVAI
                     NSLIKIIGEERVNNWASKLGKNSFTRYILLPLVSVFFLGNPMCYTFGRFLKEEHKAGF
                     YDAAVSFVHPITGLFPHANAGELFVWLGISAGLTTLGKETTTLALWYFIVGLIVIFIR
                     GIVTEKMYAFLTRKNRATNKA"
     gene            760..1773
                     /locus_tag="ACGI59_003537"
     CDS             760..1773
                     /locus_tag="ACGI59_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFG9741289.1"
                     /translation="MEENKILKIEKGTSGWGGPLYIKKEGNRNKILSMTAGGIHEVTL
                     KIKELLGCEIVDGFKTGVSDEEVAVVIIDCGGTARCGVYPKKKIPTINVNPVGKTGPL
                     AKFITEEYYVSDVNPNCISVVDGEDMPQKSQENKSENKSSIRKPDNYDEVKSKAQGEY
                     AKKNIILSIGQGAGQVVSKFYDAGRDTIQMVMNNVIPFMAFVSMLMGIILASGLGDWI
                     ARVISPLAGNIGGLLIISVICTLPFLSPILGPGAVIAQVVGTLVGTQIGLGAIPAYLA
                     LPALFAINGQAGCDFVPVGLSLGEAEPETVEYGVPALFYSRLITGPIAVIIAYGVAVF
                     ALR"
     gene            1995..3968
                     /locus_tag="ACGI59_003538"
     CDS             1995..3968
                     /locus_tag="ACGI59_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFG9741290.1"
                     /translation="MSVTEKIPSIDRKIFKILIMCSKKEFVSINSIANELNVTTRSVR
                     TYIKQLNKDLGNDIAVIKYIKGQGYKLEIKDEQILNKIIDMNRKNVFSLNSKEDRVEF
                     ILNYLIELDGFITLDSLADEMCVGRTTLVNDFQYVEKVLASYNLNLIKKQNTGMKLNG
                     NELDIRLFILNQLYKNSRKDFSNSKYFKGIKKEEIINLEEKLLTLFKKNNFYVTDEML
                     REVINYIVVLVYRVKEEKKVKDYDVKFDLLKSYDEYFIAREIKHIISGMFECTLNDEE
                     IIYLTIPLVSGNAPASECALNSSRISKNIDELMENIFNQIYVDMGIFINEDELRVGLG
                     YHLSFTLNRLLFNIKLKNVLLEEIKQNYILPFKLAQIAGKVIEKKYNLEVSEDEIGYI
                     AIHFSGYLERNSSRFYSIKKIAIICSTGLGTAKLLKIRVEKLIGNNPKIDTLSSFNLR
                     NINLDEYDIVFTTIDLDTSNINTIVLKINTIFDENKLREQLKTVLCLREGNIDTTNST
                     NLLINNLLDEDKFMILNEKTILASLEKMMDHLMDLGCIDEKFRKNIVNREEKSPTVFD
                     KGLLFPHSINEKSDKFLMAVGILEEPIEYANRSIKIILMTMFPCENKIDSDLLVKIYE
                     EVLKIGQDVKLTNKISKCRTFLEFKKVLLKNLI"
     gene            4037..4396
                     /locus_tag="ACGI59_003539"
     CDS             4037..4396
                     /locus_tag="ACGI59_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFG9741291.1"
                     /translation="MKLLVILFVAYILNTIFIMKQNKLYFKTLNRAKKKGDIVSTGKK
                     KSYFSKGSIAVISSDSDGFIKFGEILKGRTVMAKFKEIEEIEGLDIYSAEQKFKDEES
                     IVQAIKFIKEKINVSFN"
     gene            4431..4787
                     /locus_tag="ACGI59_003540"
     CDS             4431..4787
                     /locus_tag="ACGI59_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFG9741292.1"
                     /translation="MKYEVKISGIGSLVKELMDESNCLIIYDETINDDDLKDISVIHS
                     IATLKSDVEIGDTLTIGNRDYCIVSVGDIAQKTLREIGHCTIKFDGKCEVNLPGEIHV
                     EIGNPDITIGDLITIS"
     gene            4824..6116
                     /locus_tag="ACGI59_003541"
     CDS             4824..6116
                     /locus_tag="ACGI59_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFG9741293.1"
                     /translation="MLDLNRKLAKLEEEGKKIKVALIGAGHMGNGMVSQMANMKGIEA
                     SIVVDIDLELAHKAFTDAGIDEEIIDNVTNEKDAEIKLQEGKVLTCNDFLVACKTKSI
                     DVVIDATGGIAIGAEIALNSILNKKHIVMLNAETDCVVGPILKKLADDAGVIFTGSAG
                     DEPGAVMELFDFADAMGFEVRVIGKGKNNKLDLDCNPDTVREEAERKGASPHMIASFK
                     EGTKTMVEMALMCNATGFVPDVRGGHGIEATVNEVPKKYALKSEGGVLDNYGVVDFVN
                     GIAPGVFVVVAHKLKAVNDELKYLSMGDGPNYILYRPYHLCSLETPLSAAMAVLDHKA
                     TIVPKAGLVAEVMTIAKKDLKKGEFMDGYGAYTCYGTIEKYDVAKAMNAVPIGLISKK
                     TKVVKDIKKGEVITYDMVEIEKDTTLYHLRQLQEKIFG"
BASE COUNT         2484 a          586 c         1187 g         2023 t
ORIGIN      
        1 aaattctaat tagtacaatt ttttccttta taaaattgaa aaaagatgta tttatttatc
       61 aaaaatatta tattaaaatg atattaataa aaaaacattt acaatattag catatagtac
      121 agaaaaaaga tgtaatttgt aaaatttatg gtaagcagta aaattaaaaa tttaaagata
      181 ttatagggag gttataattt tatggaaaac gttattacag gtctaagtaa aggtgcagag
      241 tggtttatcg gtttgtttca gaaaggggga gaacaattta taggtcttgt ttcaggtact
      301 cttccaacgc taattgtatt aatggtagct attaactctt tgattaagat aattggtgag
      361 gaaagagtta ataattgggc ttctaagcta ggaaagaact cttttacaag atatattcta
      421 cttccattag tatcagtatt tttcttggga aatcctatgt gctatacatt tggtagattt
      481 ttaaaagaag agcacaaagc aggattttat gatgcagcag tttcatttgt acatccaatt
      541 acaggtcttt ttccacatgc aaatgcagga gaactttttg tatggttggg aatatctgca
      601 ggtttgacta ctttaggtaa agaaactact acattggcat tgtggtattt tatagttggt
      661 ctaattgtaa tttttataag aggtatagta acagaaaaaa tgtatgcttt tctaacaaga
      721 aaaaatagag caacaaataa ggcttaggag gtagataaaa tggaggagaa taaaatatta
      781 aaaatagaaa agggaacttc aggctgggga ggtcctttgt atataaaaaa agaagggaat
      841 agaaataaga tactatcaat gactgctggc ggtatccatg aagtaacatt aaaaataaaa
      901 gaactactgg gttgtgaaat tgtagatgga tttaaaactg gagtttctga tgaagaagta
      961 gcagtagtta ttattgattg tggtggaact gctagatgtg gagtttatcc aaagaaaaaa
     1021 atacctacaa taaatgtaaa tccagttgga aaaacaggtc ctttagcaaa atttataaca
     1081 gaagaatatt atgtatcaga tgttaatcct aactgtataa gtgttgtaga tggggaagat
     1141 atgccacaaa aatctcaaga aaataaaagc gaaaataaga gttctattag aaagcctgat
     1201 aattatgatg aggttaagtc aaaagcacaa ggtgaatatg ctaaaaagaa cataatactt
     1261 tctattggtc aaggggcagg gcaagtagta tcaaaatttt atgatgcagg tcgagatact
     1321 atccaaatgg taatgaataa cgtaatacca tttatggcat ttgtaagtat gcttatggga
     1381 attattttag catctggact gggagactgg attgcaagag ttatatctcc acttgcaggt
     1441 aatattggtg gattattgat tatatcagta atttgcacac ttccattctt atcaccaata
     1501 ctaggacctg gtgcagttat agctcaagta gtagggactc ttgtaggtac acaaattggt
     1561 cttggtgcaa taccagcata tttagcactt ccagctttat ttgcaataaa tgggcaagct
     1621 ggatgtgatt ttgtaccagt aggattaagt ttgggagaag cagaaccaga aactgtagag
     1681 tatggtgttc cagctttatt ttactctcgt ttaataactg gtccaatagc tgttattatt
     1741 gcatatggag ttgcagtatt tgcattgaga taatatttaa tgtcaatata aggcttggaa
     1801 ttaattaata aaattgtaaa tataaattta agctagtcaa gattttttgt taatgaaaga
     1861 ctagctttta tactattaat tagctgtaat tatcgagtta gattaatact aaattttcct
     1921 ttattaaagt gaaattttat gtattaattt ggtttcaaat ttgtactaga atgaaatcaa
     1981 ggaggagata caaaatgagt gtaactgaga aaattccttc tattgaccgc aagatattta
     2041 agatattgat tatgtgtagt aaaaaagagt ttgtgagtat taactcaata gcaaatgaat
     2101 tgaatgtcac aactagaagt gtaaggacgt atataaaaca attgaataaa gatttaggta
     2161 atgatatagc agtaatcaaa tatataaagg gtcaaggcta taaactagaa ataaaagatg
     2221 agcagatact caataagatt attgatatga atagaaaaaa tgtattttca ttaaattcaa
     2281 aagaagatag agtagagttt atactaaatt acttgataga actagatgga tttattacac
     2341 ttgatagttt agctgatgag atgtgtgtag gaagaactac tttagtaaat gatttccagt
     2401 atgtagaaaa agttttggct tcatataatt taaacttgat taaaaaacag aatacaggta
     2461 tgaaattaaa tggaaatgaa cttgatatta gactgtttat attaaatcag ttgtataaaa
     2521 actcaaggaa agatttcagt aattcaaagt attttaaagg aatcaaaaaa gaagaaatca
     2581 taaatttaga agaaaaacta ttgactttat ttaaaaaaaa taatttttat gttacagatg
     2641 aaatgttaag agaagttatt aactacatag tagttttagt ctatagagta aaagaagaaa
     2701 agaaagtaaa agattatgat gtaaagtttg atttacttaa atcatatgat gaatacttta
     2761 ttgctaggga aataaaacat ataatatcag gaatgtttga atgtacttta aatgatgaag
     2821 aaattattta tttaactata ccgttagtta gtggaaatgc accagcatca gaatgtgctt
     2881 taaatagtag tagaattagt aaaaacattg atgagttgat ggaaaatata tttaatcaaa
     2941 tatatgtcga tatgggtatt tttataaatg aggatgaatt aagagtaggt ctaggatatc
     3001 atcttagttt tactttaaac aggctattat ttaatataaa gttaaaaaat gttttgttag
     3061 aagagattaa gcaaaattat atacttccat ttaaattagc acaaatagca ggaaaagtaa
     3121 tagagaaaaa gtataactta gaagtttcag aagatgagat tggatatata gcaatccatt
     3181 tcagtggata tttagaaaga aatagcagta gattctattc aataaaaaaa atagcaatta
     3241 tatgtagtac aggtctagga acagcaaaac tattgaaaat aagagtagaa aaactgatag
     3301 gaaataatcc taaaattgat actctttcaa gttttaattt gagaaatatt aacttagatg
     3361 aatacgatat agtttttaca actattgatt tagacacatc aaatataaat acaatagtat
     3421 taaaaataaa tacaatattt gatgaaaaca aactaagaga acaattaaaa acagtattgt
     3481 gcctaagaga agggaatatt gacactacta attctacaaa tttactaata aataatttgt
     3541 tagatgaaga taaatttatg atattaaatg aaaagactat acttgcctca ttagaaaaaa
     3601 tgatggacca tcttatggat ttgggatgta ttgatgagaa gtttagaaaa aatatagtca
     3661 atagagaaga aaaatctccc acagtatttg ataaaggttt attatttcct cattctataa
     3721 atgaaaaatc agataagttt ttaatggcag ttggaatttt agaagaacca atagaatatg
     3781 caaatagaag tataaaaata attcttatga caatgtttcc atgtgagaat aagatagact
     3841 cagacttatt agtaaaaatt tatgaagaag ttttaaagat aggccaagat gtaaaattaa
     3901 cgaataagat aagtaaatgt agaacttttt tagaatttaa aaaggtgtta cttaaaaacc
     3961 ttatataact attgtacagc atttatagtt gttatacaga tttaaaaata atgtgatttt
     4021 tggaaggtgt ataaatatga aattattagt aattttattt gttgcttata ttttaaatac
     4081 tatatttata atgaagcaga ataaattgta ttttaaaact ttaaatagag ctaaaaaaaa
     4141 gggtgatata gtgtctacag gcaaaaagaa aagctatttt tctaaaggaa gtatagccgt
     4201 tatatcttca gattcagatg ggtttataaa gtttggagaa attttaaaag ggcgtacagt
     4261 aatggctaaa tttaaagaga ttgaagaaat agaaggatta gatatatata gtgcagagca
     4321 aaaatttaaa gatgaagaga gtattgtaca agctataaaa tttataaaag aaaaaataaa
     4381 tgtttctttt aattaactca atataaatat aaattatcag gaggaatgtc atgaaatatg
     4441 aagttaagat aagtgggata ggcagtttag ttaaagagtt aatggatgaa agcaattgtc
     4501 ttattatata tgatgaaacc attaatgatg acgacttaaa ggatatatct gtaattcatt
     4561 caatagcaac attaaaaagt gatgttgaaa taggagatac acttactata gggaatagag
     4621 attattgtat agtttcagtt ggtgatattg ctcagaagac tcttagagaa attgggcatt
     4681 gtacaatcaa atttgatgga aaatgtgaag taaatttacc aggagaaata catgtggaaa
     4741 taggaaatcc agatataact attggagatt taataactat ttcttaattt gaaaataaaa
     4801 ttattataac aggaggaatt ggtatgttag atttaaacag aaaacttgca aaattggaag
     4861 aggaaggtaa aaaaataaaa gtagctctta taggagctgg gcatatggga aatggaatgg
     4921 taagccaaat ggcaaatatg aaaggtattg aagcttctat tgttgtagat atagatttgg
     4981 agttggctca taaagctttt actgatgcag gaatagatga agaaattata gataatgtaa
     5041 caaatgaaaa ggatgcagaa ataaaattac aagaaggaaa agtattgaca tgtaatgact
     5101 ttcttgtggc ttgtaaaact aagtcaatag atgtagttat agatgcaact gggggtattg
     5161 caattggtgc agagatagca ttaaactcta ttttaaataa aaaacatata gttatgttaa
     5221 atgctgaaac tgattgtgtt gttggaccaa tacttaaaaa attagcagat gatgctggag
     5281 taatatttac aggttctgca ggagatgagc caggtgcagt aatggaatta tttgattttg
     5341 cagatgcaat ggggtttgaa gttagagtga taggtaaagg taaaaataat aaattagatt
     5401 tagattgtaa tcctgataca gttagagaag aagcagaaag aaaaggagct tcacctcata
     5461 tgatagcttc ttttaaagaa ggaactaaaa caatggtaga aatggcttta atgtgtaatg
     5521 caactggatt tgtgcctgat gtaagaggtg gtcatggtat tgaagctaca gttaatgaag
     5581 tacctaagaa atatgcatta aaaagcgaag gtggagtatt agataattat ggtgtagttg
     5641 attttgtaaa tggtatagca cctggagttt tcgtagtagt tgcccataaa ttaaaagcag
     5701 ttaatgatga actaaaatat ttaagtatgg gtgatggacc taattacata ttatatagac
     5761 catatcattt atgtagttta gagactcctt tatcagcagc tatggcagtt ttagaccata
     5821 aggctacaat agtacctaaa gctggactag tagcagaagt tatgactata gctaagaaag
     5881 atttgaaaaa aggtgagttt atggatggct atggagcata tacatgctat ggtactatag
     5941 aaaaatacga tgtagcaaag gctatgaatg ctgtacctat tggtttgata agtaaaaaga
     6001 ctaaagttgt aaaagatatt aaaaaaggtg aagttataac ttacgatatg gtagagatag
     6061 aaaaagatac tactttatat catttaagac aattgcaaga aaaaatattt gggtaagatt
     6121 tgatggtaat ataaatagat attagtaaaa agggttgatg gttattttta aatcaaccct
     6181 tttaaatttt tatatattct tgttaggata ctttctttaa aagtatatta taggttattt
     6241 gtagtcgtaa aaattatttt gaattagttc tgcaatttta
//
LOCUS       DAYAPA010000172         6192 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.177, whole genome shotgun sequence.
ACCESSION   DAYAPA010000172 DAYAPA010000000
VERSION     DAYAPA010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6192
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.177"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            114..764
                     /locus_tag="ACGI59_003542"
     CDS             114..764
                     /locus_tag="ACGI59_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HFG9741294.1"
                     /translation="MKAEKTSIYPIIKNNLNKISIVEYKKGQRFVASDKDLQEVFFII
                     EGVALVECTTRGGNKFLVDIVPENEFIGKISYIYEHNLKCDIFAKTNIKLFRFEKNVF
                     EEFYLKPDFIALFHRKCTRRIYELYKTRMVRELFSCTEVIAYCISNKQENNVCNIKAI
                     RNFSEIYSISRKSRYHSLLKLTEKEIVKKMGNTYEILNYDELHKLAFEVKEFLEDE"
     gene            complement(904..2280)
                     /locus_tag="ACGI59_003543"
     CDS             complement(904..2280)
                     /locus_tag="ACGI59_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFG9741295.1"
                     /translation="MQELFSLKDENKRFYKILLSLCIPIIIQNLISTSVNVIDTIMIS
                     SLGETSVASVGVANQFFFLFNMSLSGITGGAGVFISQFYGKKDVSNIRKVTGLTCVLA
                     IVLSFVFVIPALLTPKPIIHIFSYDSEVVKLCIDYFSIAVFSYPLIAVSTVFSTGSRG
                     VRNPKLGMICSAFALVTNVILNYGFIFGNFGLPALGVKGAALATVIARICELILMITY
                     VYLYKKDYILKFGLKNLKAIDKIFIKSFSSKSFPIFVNDSVWAIGTVLYSVAYARAGT
                     SAIAASQIATSTGNFFIMTAVCIASGASIMLGNELGADHIKRAIEYAKKFSILVFSAG
                     LILGIILILNIPLLLKMFSVSDSLASDITKIFFIMGILMALKSFNTLVIIGILRSGGD
                     TKYALFLELGCMWLASIPLTFIAAFKGAPIFVLVLLTYSEEVVKFIFGVPRALSKKWA
                     INIVKEID"
     gene            complement(2296..4209)
                     /locus_tag="ACGI59_003544"
     CDS             complement(2296..4209)
                     /locus_tag="ACGI59_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFG9741296.1"
                     /translation="MIVLSCNNLNKSFGIDSILENISFTVNEGDKIGIIGVNGTGKTT
                     LFKIISGIYGYDSGDIYTSKDCEIGYLEQNTNFYSDNTILEEVLEVFKNLIEMESYLR
                     ELEVKISEESTKTNSPIIEKIMDEYSHKLELFSDLNGYGYKSEAKGVLKGLGFSDNDM
                     DKPISILSGGEKTRVLLGKLLLKKPTLLLLDEPTNHLDSEAIEWLEVFLKQYKGTVML
                     ISHDRYFLDQSVNRIFEVHNKKLKVYNGNYSKFVELSKIEKELELKKFEDQQKEIKKQ
                     EESIERLKAYGREKHLKRARSKEKALDKVDVLDKPEAYRKKARIQFTPSVQSGNDVLQ
                     IRDVSMGYGERILFKDLDLDIYRGEKVALIGANGVGKSTLFKIITNELQPLSGNIKFG
                     TNVHVSYFHQEQKTLNLDNTIIDEIWENNTHLTQTTLRNMLGAFLFVDEEVFKKISTL
                     SGGERARVAILKLILSNANLLLLDEPTNHLDIDSKEVLEEALTNYDGTIFTISHDRYF
                     LNTVVDKILVLDENGITEYLGNYDYYIDKKRQIQEMSIIEEKEEKTRTQIKDEKRKER
                     EQREIEKKNRIKRQNIEKEIEKLEIEIEKLDILLCQEEVYSNPDKAKEVSQEKINLEN
                     NLASLYDEWEEFM"
     gene            4345..4977
                     /locus_tag="ACGI59_003545"
     CDS             4345..4977
                     /locus_tag="ACGI59_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFG9741297.1"
                     /translation="MLGNKNISMAVIRRLPKYHRYLGDLLDRDIQRISSKELSDIIGF
                     TASQIRQDLNNFGGFGQQGYGYNVEALHTEIGKILGLDRPYNAVLVGAGNLGQAIANY
                     AGFRKAGFEIKALFDANPRMIGLKIREFEVLDSDTLEDFIKNNNIDIAVLCIPKNGAQ
                     EVINRVVKAGIKGVWNFAPLDLEVPKGVIVENVNLTESLFTLSYLMKEGK"
     gene            5116..5655
                     /locus_tag="ACGI59_003546"
     CDS             5116..5655
                     /locus_tag="ACGI59_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdE family protein"
                     /protein_id="HFG9741298.1"
                     /translation="MVFVEELWKKAIEFHGHECPGLAIGFKAALAAREYLGCKQSGDE
                     EIVCISENDACGIDGIQVVLSCTMGKGNLLLRMTGKSVYHFFNRENGKSIRIYVKSKP
                     FQMDREGYKKYLLESDFEEIFEITDTKLRLPEKARIFKSLKCEVCGEFASEQYIRIEE
                     GKKVCLDCFNKYERFYEEY"
     gene            complement(5831..6001)
                     /locus_tag="ACGI59_003547"
     CDS             complement(5831..6001)
                     /locus_tag="ACGI59_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HFG9741299.1"
                     /translation="MAYKITDACISCGACEAECPVSCISAGDDAYVIDAGSCIDCGSC
                     AGACPVDAPQPE"
BASE COUNT         2291 a          859 c          862 g         2180 t
ORIGIN      
        1 atgaatttgt caagtttaat ttagttgtaa ttttatcttt tattaaaaat aatataaatt
       61 aaaacatata aataaaatat tttatacaat atattgtaaa ggaggtaact ttgatgaaag
      121 cagaaaaaac aagtatatat ccaattatta aaaataattt aaacaaaata tcaattgttg
      181 aatataaaaa gggtcaaaga tttgtagcat cagataaaga cttacaagaa gtgttcttta
      241 taatagaggg agttgcatta gtggagtgta ctactagagg aggaaataaa tttcttgttg
      301 acattgtacc tgaaaatgaa tttataggca aaattagtta catatatgaa cataatttga
      361 aatgtgatat atttgcaaag accaatataa aattatttcg atttgaaaaa aatgtatttg
      421 aagagtttta tctcaaacca gattttattg cactatttca cagaaaatgt acaagaagaa
      481 tatatgaatt atataaaacc agaatggtaa gagaactatt ttcttgcaca gaagttattg
      541 cctattgtat atcaaataaa caagaaaata atgtctgcaa tataaaagct attcgtaatt
      601 tcagtgaaat ctattcaatt agtagaaaaa gtagatacca ttctctgcta aagttaactg
      661 aaaaggagat agtaaaaaaa atgggtaata catatgaaat tttaaactat gatgaattac
      721 ataaattggc atttgaagta aaagaatttt tggaagatga gtaaaaattg ttacttaatc
      781 aaaataaagt ataataattt ctgtaaaaaa tagaaatagt ctcactgaaa atcaaaaata
      841 ctttagttat tttcaatgag actgtttaga aatattgtat acttataatg tatagtataa
      901 ttattaatca atttccttta ctatattaat agcccatttt ttagataatg ctctaggaac
      961 accaaatata aacttaacca cttcttcact ataagtgagc aaaacgagta caaatatagg
     1021 tgcaccttta aatgcagcta taaaagttag aggaatagaa gctaaccaca tacatcctaa
     1081 ttctaaaaat agagcatatt tagtatctcc tccacttctt aaaataccaa ttataaccaa
     1141 tgtattaaaa gatttaagag ccattagtat tcccattata aagaatatct tagttatatc
     1201 agaagctaag ctatcagaaa cactaaacat ttttaataat aatggtatat ttaaaattaa
     1261 aataattcca agtattaaac cagctgaaaa tacaagtata gaaaactttt tagcatattc
     1321 aatggctctt ttaatatggt cagctccaag ttcatttcca agcataatag atgcaccaga
     1381 tgcaatacat acagctgtca ttatgaagaa attacctgta ctggttgcta tctggctagc
     1441 tgctatagca gaagtaccag ctcttgcata tgcaacagaa tacaggacag ttccaattgc
     1501 ccatacagag tcatttacaa atataggaaa gctttttgat gaaaaagatt ttataaaaat
     1561 tttatcaata gcttttagat tttttagacc aaactttaat atatagtctt ttttataaag
     1621 gtaaacataa gtaatcatta aaattaattc acatattcta gcaataacag tagctagagc
     1681 agcacctttt acacccaatg caggaagacc gaagttacca aatataaatc cataatttaa
     1741 aataacattt gttacaagtg caaatgcact acaaatcata cctaacttag gatttctaac
     1801 acctcttgaa ccagtgctaa atacagtact tacagcaatt aatggataac taaatactgc
     1861 aatactaaag taatctatac aaagttttac cacctcagaa tcatacgaaa atatgtgtat
     1921 aattggcttt ggtgttagta aagcaggaat aacaaataca aaacttaaaa caattgccaa
     1981 tacacaagta agacctgtga cttttcttat attactcaca tccttctttc cataaaactg
     2041 tgaaataaaa actccagcac ctcctgtgat accagataag gacatattaa aaagaaagaa
     2101 aaattggttt gcaactccaa ctgaagctac agatgtttcc cccaaactac ttatcataat
     2161 tgtatctatt acatttacag aagtagaaat taagttctgt atgataatag gtatacataa
     2221 agatagaagt attttataaa atcttttatt ttcgtctttt aaagaaaata gttcttgcaa
     2281 ataatcctct cctttttaca taaattcttc ccactcatca tatagagagg caaggttatt
     2341 ttctaaatta attttttctt ggctaacttc tttagcttta tctggattag agtatacttc
     2401 ttcttgacat aaaagtatat ctaatttttc aatttcaatc tctaactttt caatttcttt
     2461 ttctatattt tgtcttttta ttctattttt cttttctatt tctctttgtt ctctctcttt
     2521 tcttttttca tcttttattt gagttctagt tttttcttct ttttcttcta taatactcat
     2581 ctcttggatt tgcctttttt tatctatata ataatcataa tttcccagat attcagtaat
     2641 tccattttca tctaaaacta aaattttatc aacaacagta tttagaaagt atctatcatg
     2701 tgatattgta aatatagtcc catcataatt agttaatgcc tcttcaagga cttccttaga
     2761 atcaatatct aggtgattag taggctcatc taataacaat aagtttgcat ttgatagtat
     2821 aagtttaagt atggcaactc tagctctttc tccaccactc aatgtagaaa tcttcttaaa
     2881 aacttcttca tctacaaata aaaatgcccc taacatattt ctcaaagtag tttgagtaag
     2941 atgagtatta ttttcccaaa tctcatcaat tattgtgtta tctaaattaa gagttttttg
     3001 ttcctgatgg aagtaggaaa catgtacatt agttccaaat ttaatattgc cactaagagg
     3061 ttgtagttca ttagttatta ttttaaataa tgtagatttg ccaacaccat ttgcacctat
     3121 aagtgcaact ttttcccctc tatatatatc taaatccaag tctttaaaaa gtattctttc
     3181 tccataaccc atagatacat ctctaatttg aagaacatca ttaccactct ggacagaagg
     3241 cgtaaattgt atacgagctt ttttcctata agcttctggt ttatctaaaa cgtctacctt
     3301 gtctaatgct ttttctttac ttcttgcacg ctttaggtgt ttttctctac cataagcttt
     3361 tagtctttca attgattctt cttgcttttt tatttctttt tgttggtctt caaatttctt
     3421 aagctctagt tctttttcta ttttagataa ctcaacaaat ttagagtagt taccattata
     3481 tactttaagt tttttgttgt gaacttcaaa aattctattt acggattggt ctaagaaata
     3541 tctatcatga gatattaaca tgacagtccc tttatattgt tttaaaaata cttctaacca
     3601 ttctattgct tcagagtcta agtggttagt aggttcatca agtaacaata gagttggttt
     3661 cttaagaagt aattttccta aaagaactct agttttttca ccaccagaaa gtatgcttat
     3721 tggcttatcc atatcattgt cactaaatcc taatcccttt agaactcctt tggcttcaga
     3781 tttatatcca tatccattta aatctgaaaa taattctagt ttatgtgaat attcatccat
     3841 gattttttct ataattggag agtttgtttt tgtactttct tcagatattt taacctccaa
     3901 ttctcttaaa taggattcca tttctatcaa atttttaaaa acttctagta cttcttccaa
     3961 aatagtatta tccgaataaa aattggtatt ttgttctaaa tatccgattt cacaatcttt
     4021 agaagtgtaa atatctccac tatcatatcc ataaattcct gaaattattt taaataaagt
     4081 agtttttcca gtaccattaa caccgattat accaatttta tcgccttcat ttactgtaaa
     4141 actaatattt tctaatatag aatctatacc aaaactttta tttaagttat tacatgacaa
     4201 aacaatcata aatttctacc tccttgaact atctaaaatt ataatgtttt gatataatat
     4261 aatcaaggtt atatgtatta gttttgaaaa agaaatattt tattgtgaat caaacaaaaa
     4321 agaggacact tttaggaggt gaagatgttg ggaaataaaa atatatcaat ggcagttata
     4381 agaaggctcc caaaatatca tagatatctt ggagacttat tagataggga tatacaaaga
     4441 atatcttcta aagaattgag tgatataata gggtttaccg cttctcaaat aagacaagat
     4501 ttaaacaact ttggtggatt tggacaacaa ggatatggtt ataatgtaga agctcttcat
     4561 actgagatag gtaaaattct tgggttggat cgaccataca acgcagttct tgtaggagca
     4621 ggtaacttag gacaagctat agccaattat gcaggattta gaaaagctgg attcgagata
     4681 aaagctttat ttgatgcaaa tcctagaatg ataggtttaa agataagaga gtttgaagta
     4741 ttagattcag atactttaga agactttata aaaaacaata atatagatat tgctgtatta
     4801 tgtataccta aaaatggagc acaagaagtt attaatagag ttgtaaaagc tggaatcaaa
     4861 ggtgtatgga attttgcacc tttagattta gaagttccga aaggtgttat agttgaaaat
     4921 gtaaacttaa cagaaagttt atttacctta tcgtatttaa tgaaagaagg aaagtagaca
     4981 acttaaacat attaataatg agcagttgtg aagagtattg agaaagtcag aagatttaat
     5041 attaaatcct ctggcttttt gaaatattag gataaaaatt taaataatgt atggaaataa
     5101 acaatattaa ggagagtggt ttttgtggag gaattatgga aaaaggcaat tgaatttcat
     5161 ggtcatgaat gtccagggtt agctatagga tttaaagcag cacttgcagc aagagaatat
     5221 ttaggatgta aacagtcagg agatgaggaa attgtatgta tttctgaaaa tgatgcttgt
     5281 ggaatagatg gtatacaagt cgtgctaagt tgcacaatgg gtaaaggaaa tttactactt
     5341 aggatgactg ggaaatcagt atatcatttc tttaataggg aaaatggaaa atctataaga
     5401 atatatgtaa agtcaaagcc atttcaaatg gatagagaag gatacaaaaa gtacttatta
     5461 gaaagtgatt ttgaagaaat atttgagatt acagatacta agttaagact tcctgaaaaa
     5521 gcaagaatat ttaaatctct taaatgtgaa gtgtgtggtg aatttgcatc tgagcaatat
     5581 ataaggattg aagaaggaaa gaaagtatgt cttgattgct ttaataaata tgaaagattt
     5641 tatgaggagt attaaaatta taaattagat taaagttaaa agtattagat atgtgctaac
     5701 agtattttaa gatgaattag gtagcaacat aataaagtat ataaataata ttaattgaat
     5761 catacataat taaaaagagg ctgattagta tttataaaat aaaatcagtc tctttatgaa
     5821 taacgaatta ttattctggt tgaggtgcat ctacaggaca agctcctgca caagaaccac
     5881 agtctataca agaaccagca tctataacat atgcgtcgtc tccagctgat atacagctaa
     5941 cagggcattc agcctcacaa gcaccacagc ttatacaagc gtcagtaatt ttatatgcca
     6001 ttttgaaatc ctccctttta tatgtattgt aattcaataa cattataaca aatattattg
     6061 aatataacaa ttaaattaat tctacaacaa aaactttatt atgatattct tgttttaaag
     6121 ttttacaata aagtttaatc tgcttatatt tttatttatt ttaaagaagt ttattttaaa
     6181 aataaaatta aa
//
LOCUS       DAYAPA010000173         6008 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.178, whole genome shotgun sequence.
ACCESSION   DAYAPA010000173 DAYAPA010000000
VERSION     DAYAPA010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6008
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.178"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            190..930
                     /locus_tag="ACGI59_003548"
     CDS             190..930
                     /locus_tag="ACGI59_003548"
                     /inference="COORDINATES: protein motif:HMM:NF024744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HFG9741300.1"
                     /translation="MKNFRDLGGNKTEDGRTVKKGLFYRSAKLSNLSENDIKILKELN
                     IKYIFDYRSDEEARKHPSTIISNIKNIRIPAMRELEESGGSFGSIEDMIDGLFEKDGA
                     FNMLNNSYYNLPINNPSYKKLVELIRDYSNLPILNHCTAGKDRTGVGSAIILMILGVS
                     RENIMKDYLKSNDFADKEIERFIEYKPKFKDIPKENLKYIFGVNEEYMKTAFRRIDEE
                     YISVEAYLYGEFNLNKEEIRKLRNQYLE"
     gene            942..2720
                     /locus_tag="ACGI59_003549"
     CDS             942..2720
                     /locus_tag="ACGI59_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFG9741301.1"
                     /translation="MSLNITARQINIIKILLNSKDTISGIALSQEIGCSSKTIQNEIK
                     DINKQLRNGKILSIRGVGYKLEGSFDEVNLNSNLYDDVDRVEYIIRKILTLSNEEKNT
                     IKLEDLADSMYVSVSTVKNDLKEVKRMLEKYSISVKSKHKQGICVLESEDKILNCIVD
                     LSNKRDNQLSLNDFLNDDIKERKLLIKKLLLDILNENGLVLTDIEFKSVLNNILVMLS
                     RHKYNEKEFIENYIENYRSKRSSILENEQNKEIIINSIKTFCKNLKLATSIDISNDKV
                     FEEFLYKHISSLCKKIKLGINQSPIMAQDIKIKYPFAFELANIAKKTIEKDLKIEINE
                     DEVANIALHIGGAVERASYNNKDKVFKTIIVCTSGIGTSMLIKAKLENIFKEKLEILK
                     IIPSYLVEYIKVIDVDFVISTVPIEVGDVPVINISPILSEKEIRLIEKYIETGNIYLD
                     LDIKSLFDSELFFTDLVFKRKEEVIDFMASKLVQKGYIDEDMKKSYFEREKIATTEIG
                     NMVTIPHGAIGKIFDNKIAIGILKKPISWEISDVRLIIMLALDKDKILDYEVIFSKIY
                     KRVDSIAKVISICENSSFEKFVNLFN"
     gene            2887..3804
                     /gene="pfkB"
                     /locus_tag="ACGI59_003550"
     CDS             2887..3804
                     /gene="pfkB"
                     /locus_tag="ACGI59_003550"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFG9741302.1"
                     /translation="MIYTVTLNPSIDYIVKLDELKTGSTNRVKEEYVYPGGKGINVSR
                     IFKELGNDNTSLGFISGFTGEYIIRTLEEKELKTDFIKIKSGFSRINVKIKALKETEI
                     NGQGPNIDDEDIDTLYKKLDKLNQDDILILAGSIPSTLDEKLYENIMARLEKKNIKVV
                     VDATKNLLLNVLKYKPFLIKPNNDELEELFGVKLNSIEDMVKYARRLKEMGAINVLVS
                     MGKDGALLITEEDEVLISDVPKGKVKNSVGAGDSMVAGFISGYLNTGKYDYALKLGAA
                     SGSATAFSYDLAKREYIDKLVNEISVKQF"
     gene            3841..5760
                     /locus_tag="ACGI59_003551"
     CDS             3841..5760
                     /locus_tag="ACGI59_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFG9741303.1"
                     /translation="MKIIDLLDEKSIKLNLNSKTKSEAIEELVDLVANSGNLNDKENY
                     KKAILAREEMSTTGIGEGVAIPHAKNSSVTKACIAAAVSKEGIDYESFDGSLSNLFFM
                     IAAPDGANNTHLEVLSRLSTILMDEDFRNKLINSSSEKEFKEIIDKKEREKFSEEYQD
                     EKVTEKNIIDTKENDANKYPKVLAVTACPTGIAHTFMAAESLNKMAENKGVSIKVETN
                     GSAGVKNKLTKDEIENATCIIVAADKNVEMARFNGKKVIKTKVADGIHKAEELIDKAV
                     NGDAPIYHGGDGSHNESNEESESGFRKVYKHLMNGVSNMLPFVIGGGILIAIAFLLDD
                     YTINPSNFGSNTPIAAFFKGIGDKAFGFMLPVLAGYIAYSISDRPAFVVGFVGGALAG
                     DGGSGFLGALLAGFIAGYLVEGLKKIFSVLPASLEGIKPVLLYPLLGTLLMGIIMTFL
                     VIPPVTAINNAMVGFLNGLGGTSKIVLGLVLGGMMAVDMGGPVNKAAYVFGVASLESG
                     QFEIMAAVMAGGMVPPLAIALATTFFKNRFTKEERDSGKVNYVMGLSFVTEGAIPFAA
                     GDPLHVIPACVGGSAVAGALSMLFNAALRAPHGGVFVIPVVTHPFAYILAIAVGALVG
                     MMLLALLKKPLNQEV"
BASE COUNT         2480 a          546 c         1096 g         1886 t
ORIGIN      
        1 tataaagctt atgtaaagta aataaattat gtaaaattta ggttaatttt gtataaagag
       61 atacttttat attttacaat taataaccaa atactaaaaa atattttaaa taaagaactt
      121 aaaattgtga taaaattaat ttatagatat taaaaaaagt ataatatatg gtttttaaga
      181 ggtgctaaga tgaaaaattt tagagattta ggtgggaaca aaactgagga tggcagaact
      241 gtaaagaagg gattatttta taggtctgca aaattatcaa atctaagtga aaatgatata
      301 aagattttaa aagagttaaa tattaaatat atatttgatt atagaagtga tgaagaagct
      361 cgtaaacatc catcaacaat aatatcaaat ataaaaaata taagaatacc agcaatgaga
      421 gagctagaag aatcaggagg aagttttggt tctattgaag atatgattga tggattgttt
      481 gaaaaagatg gtgcatttaa tatgttaaat aatagttact ataatttgcc tataaataat
      541 ccgtcatata aaaaattagt agaacttata agagactatt ctaatttacc aatattaaat
      601 cattgtacag ctggtaagga tagaactggt gtggggagtg ctataattct tatgatttta
      661 ggggtatcga gagagaatat aatgaaagat tatctaaaaa gtaatgattt tgcagataaa
      721 gagatagaaa gatttataga atataagcct aaatttaagg acattccaaa agaaaatttg
      781 aaatatatat ttggtgtaaa tgaagagtat atgaaaactg catttagaag aattgatgaa
      841 gaatacatta gtgtagaagc atatttatat ggagaattta atttaaataa agaagaaata
      901 agaaaattaa gaaatcaata tctagagtaa aaggtgagtt tatgagttta aatataacag
      961 caagacagat aaatataata aaaatattgt taaattcaaa agatacaatt agtggtattg
     1021 ccttaagtca agagatagga tgttcttcaa aaactataca aaatgaaata aaggatatta
     1081 ataaacaact tagaaatggt aaaattttgt caataagagg agttggttat aaactagaag
     1141 gaagttttga tgaggtaaat ctaaatagca atctctatga tgatgtagat agagtagaat
     1201 atataatcag aaaaatatta accttaagta atgaagaaaa aaatactata aaacttgaag
     1261 atttagccga ttctatgtat gtaagtgttt caacagtaaa aaatgattta aaagaagtaa
     1321 aaagaatgtt ggaaaagtat tcaatatcag ttaaatctaa gcataaacaa ggtatatgtg
     1381 ttttggaaag tgaagataag atattaaatt gtatagttga cttaagcaat aagagagata
     1441 atcaattgag tttaaatgat tttttaaatg atgatataaa ggaaagaaaa ctattaataa
     1501 aaaaattatt attagacata ttaaatgaaa atggattagt attaactgat atagaattta
     1561 agagtgtgtt aaataatata ttagttatgt taagcagaca taagtataat gaaaaagaat
     1621 ttatagagaa ttatatagaa aattatagga gcaaaagaag ctcaatttta gaaaatgaac
     1681 aaaacaaaga aattataata aattcaataa aaacattttg taaaaattta aaattagcta
     1741 catcaataga tataagtaat gataaggtat ttgaagagtt tttgtataag catattagta
     1801 gtttatgtaa aaaaataaag ttaggtataa atcaatcacc tataatggct caagatataa
     1861 aaataaaata tccatttgct tttgaactag ctaatatagc taaaaagaca atagaaaaag
     1921 atttgaagat agaaattaat gaagatgaag ttgcaaacat agctctacat ataggaggag
     1981 cagttgaaag agcatcttat aataataaag ataaggtttt caaaactata atagtttgta
     2041 cttcaggcat aggcacatca atgttaataa aagctaaatt agagaatata tttaaggaaa
     2101 aattagaaat actaaagatt attccttcat acttagttga atatataaaa gttatagatg
     2161 ttgattttgt aatatctact gttcctattg aagtaggaga tgtgccagta ataaatatat
     2221 ctccaatttt aagtgagaaa gaaattagat taattgaaaa atatatagaa acaggaaata
     2281 tatacctaga tttagatata aaaagcctgt ttgatagtga attgtttttt actgatttgg
     2341 tgtttaaaag gaaagaagaa gttatagatt ttatggcaag taaacttgta caaaagggct
     2401 atatagatga ggatatgaaa aaatcttact ttgagagaga aaaaatagca acaacagaaa
     2461 tagggaatat ggtaactata ccacatggag ctattggaaa aatttttgat aataaaatag
     2521 caattggtat attaaagaag cctatttctt gggaaatatc agatgttcgt ttaataataa
     2581 tgttagccct tgataaagat aaaatattag attatgaagt aatattttca aaaatatata
     2641 aaagagtaga ttctatagca aaagttataa gtatatgtga aaatagtagt tttgaaaaat
     2701 ttgtaaattt attcaattag atgggtagta ttgtatatac acctgtaaat ttggataata
     2761 taaaactaaa taagataaaa acgataaatt tctcttttaa ttaagagaaa ttttttgact
     2821 ttcaaggata ttaatataaa gttaagatat aaacataaag caaataaata aaaaagaggt
     2881 ggcaatatga tttatacagt gacattaaat ccatcaatag attatatagt taagctagat
     2941 gagctaaaga caggaagtac aaatcgtgta aaagaagaat acgtttatcc aggaggaaaa
     3001 ggtataaatg tatctcgcat atttaaggaa cttggaaatg ataatacatc tttaggtttt
     3061 ataagtggtt ttactggaga atatataata agaacattgg aagaaaaaga attgaagaca
     3121 gattttataa agataaaaag tggtttttca agaataaatg taaaaataaa ggcgttaaag
     3181 gaaactgaga ttaatggaca aggtccaaat atagatgatg aagacattga tactttatat
     3241 aaaaaacttg ataagctaaa tcaagatgat attttaatat tggcaggaag tataccatct
     3301 actttagatg aaaaactata cgaaaatata atggctagat tagaaaaaaa gaatataaaa
     3361 gttgtagtag atgcaactaa aaatcttctt cttaatgttt taaaatataa accttttcta
     3421 attaagccta ataacgacga attagaagaa ctatttggtg ttaaattaaa ttcaattgaa
     3481 gacatggtaa aatatgctag aaggttaaag gaaatgggag ctataaatgt cttggtatca
     3541 atgggaaaag atggagcatt gttgattaca gaggaagatg aagtgcttat aagtgatgtt
     3601 ccaaaaggaa aagtaaaaaa ttctgtagga gcaggtgatt caatggtagc aggatttata
     3661 tctgggtatt tgaatactgg gaaatatgat tatgctctta aattgggagc agcttcaggt
     3721 agtgcaactg ctttttcata tgacttagcc aaaagagaat acattgataa attggtaaat
     3781 gagatatctg taaagcagtt ttaaaatatt aaaaaatata tagaaaggta attaatattt
     3841 atgaaaatta tagatttatt agatgaaaag tcaattaagt taaatttaaa ttcaaaaacc
     3901 aaatcagaag caatagaaga attagtagat ttggtcgcaa atagtggaaa tttaaatgat
     3961 aaagaaaact acaaaaaagc aattttggct cgtgaagaaa tgagtacaac aggaattgga
     4021 gaaggtgtag ctatacctca tgctaaaaat tcttcagtta ctaaagcttg tatagcagca
     4081 gcagtgtcaa aagaaggtat agattatgaa tcgtttgacg gaagtttatc aaatctattt
     4141 tttatgatag ctgcaccaga cggagctaac aatactcatc ttgaagtatt atcaagatta
     4201 tctacaatac ttatggatga agactttaga aataaactta taaattcaag ctctgaaaaa
     4261 gaatttaagg aaataataga taaaaaggaa agagaaaaat tcagtgaaga atatcaagat
     4321 gagaaagtta ctgaaaaaaa tataatagat actaaagaga atgatgctaa taagtatcct
     4381 aaggttttag cagtaacagc atgtccaact ggaatagcac atacatttat ggcagctgaa
     4441 agtttaaata aaatggcaga aaacaaaggt gtatcaataa aagtagaaac taatggttca
     4501 gcaggtgtta aaaataagct tacaaaagat gaaatagaaa atgcaacatg tataatagtg
     4561 gcggcagata aaaatgttga gatggctaga tttaatggta aaaaagtcat aaaaactaag
     4621 gtggctgatg gtatacacaa agcggaagaa cttatagaca aggctgtaaa tggagatgct
     4681 ccaatttacc atggaggaga tggaagtcac aacgaatcaa atgaagaaag tgaaagtgga
     4741 tttagaaaag tatacaaaca cttaatgaat ggtgtatcta atatgttacc atttgttatt
     4801 ggtggaggga tacttatagc gatagccttt ttacttgatg attatactat taacccttca
     4861 aactttggtt caaatacacc tatagcagca ttttttaaag gaataggaga taaagctttt
     4921 ggatttatgt tacctgtact tgctggatat atagcatact ctatatctga tagaccagcg
     4981 tttgttgtag gatttgtagg aggtgctcta gcaggtgatg gtggttcagg ttttttaggt
     5041 gcactacttg caggttttat agcaggttat ttagtagaag ggcttaagaa aatatttagt
     5101 gtactaccag cttcattgga aggaataaaa ccagtattgt tatacccttt acttggaaca
     5161 cttttgatgg gaattataat gacattttta gtaatacctc cagttacagc aattaataat
     5221 gcaatggttg gatttttaaa tggtttaggt ggaacttcaa aaatagtttt aggattagta
     5281 ttaggtggaa tgatggctgt ggatatgggt ggacctgtaa ataaagcagc atatgtattt
     5341 ggagttgcat cacttgaatc agggcagttt gaaataatgg cagcagtaat ggctggtggt
     5401 atggttccgc cacttgctat agctcttgca actacatttt ttaagaatag atttactaaa
     5461 gaagaaagag attctggtaa agttaattat gtaatgggat tatcatttgt tactgaagga
     5521 gctatacctt ttgcagctgg agacccactt catgttatac cagcttgtgt tggaggttca
     5581 gcagtggcag gagcacttag tatgttgttc aacgcagctc ttcgtgcacc tcatggagga
     5641 gtatttgtta taccagtggt tacccatcca tttgcatata tattagctat agctgtaggt
     5701 gctttggttg gaatgatgct tttagcatta cttaaaaaac cattaaatca agaagtatag
     5761 caattaaagg atattcaaga acttaaaata tgatgtttta taaaaattaa tatattgctg
     5821 aattatttta aaataaggat tttatgaggc tgttatataa cgatttgtaa ttgttatctt
     5881 gacagtcttt tatttttata taagtaaaat aagtgtgttt tgctttattt gtatttaaat
     5941 tgtttttaag ttagaatata aagaaaatta tatattattt taatattcac tcgaactaga
     6001 aaataata
//
LOCUS       DAYAPA010000174         5773 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.179, whole genome shotgun sequence.
ACCESSION   DAYAPA010000174 DAYAPA010000000
VERSION     DAYAPA010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5773
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.179"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            77..2434
                     /locus_tag="ACGI59_003552"
     CDS             77..2434
                     /locus_tag="ACGI59_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HFG9741304.1"
                     /translation="MDKVNLERYSCDINVGLNKNQLQSRIDDNLINAFDNGKTKTYKQ
                     IFKDNIFTLFNLINIVLVCLLVSVGSFKNTLFIFIAVINTIIGVIQEIRAKRLLDNLS
                     VIVSNKVSVIREKEKKEIDVQELVLDDIMILKTGNQICADSKVLNGKLEVNESLVTGE
                     SDIIIKNKGDFLYSGSFVVSGEAFVRVENIGKDNYANKIANDAKISKKHNSQLRNSLN
                     AILKIVGIIIIPLGIILFAKQHFGNGDSIATSVVGTVAAVNGMIPEGLTLLTSIALAV
                     GTIKLASHKTLVQELYCVETLARVDTLCLDKTGTITEGKMQVDDIVMLEEVDINEIMG
                     NICNSLKDDNATLNAIRDKYNVLDTYKAKNLIPFSSERKYSGVTFENKGTYLLGAFEF
                     IFKEKNKKLRIEVERYSKKGNRVIVLAHSDSYMEDNNIPEDIKPLAFILILDKIRDEA
                     KETLEFFYNEGVDIKIISGDNPITVSEVARKAGLKTASNFIDATTLKNDSDIYKAVDK
                     YSVFGRVSPQQKKQMILALKKQNHTVAMTGDGVNDVLALKEADCSIAMASGSDVTKNV
                     SNLVLLDSNFASMPKVVMEGRKVINNIQRASSLFLVKTIFSFFLSLLTLFFFSRQYPF
                     VPIQLSLIGAVTVGIPSFFLALEANKSRISGNFLLNILKNALPGALCVTVNVIIVYNI
                     VEYLGYSSSVFSTMCAILTGVCGLLILRQQCLPFNKERLFLIVSMTIAFVIGILFLGG
                     LFSFVALSRELILITMALAILMPIMIKVMLFVLDEILETIVPDLN"
     gene            complement(2520..2837)
                     /locus_tag="ACGI59_003553"
     CDS             complement(2520..2837)
                     /locus_tag="ACGI59_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HFG9741305.1"
                     /translation="MSEFKNVTAVKKANVYFDGKVSSRVIILPNGERKTLGLMLPGEY
                     TFSTREEEIMEMLAGSMDVKLPGSNEFVTYKEGQKFNVPSDSSFDLKVNEVVDYCCSY
                     IAD"
     gene            complement(3421..4692)
                     /locus_tag="ACGI59_003554"
     CDS             complement(3421..4692)
                     /locus_tag="ACGI59_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFG9741306.1"
                     /translation="MLKVLKGNIIFTKTSETFTVFENSYIMLEKGKVKGIFKEIPKEY
                     KNLKVVDYTDKLIIPGMNDLHAHASQFKNLGMAMDKELLPWLETYTFPEESNYKDIEY
                     ATKMYKKFVKELWKSGTTRIAVFATVHKEASMKLMDIFKEVGLGAYVGKVNMNMNCPD
                     TLLENTQDSITDTEEIINKYSDTDSIVKPIITPRFIPSCTSELLKGLGDLCLKYNIPI
                     QSHLSENYDEIEWVRSLEPESKFYGDAYNRYNLFGQTPTLMAHCVHCSQDEIDLMREN
                     NVMAVHCPASNFNVGSGAMPIRKLIDNNIRLALGSDISGGHTLSIFKAMVSAIQLSKL
                     YWVNSGKKYNFLSLSEAFYIATKSGGSFFGKVGSFEEGYDFDALIIDDSNLNHDNYSI
                     LERLERFIYVGDDRNIIHRYVCGKLIEEPNI"
BASE COUNT         2148 a          674 c          832 g         2119 t
ORIGIN      
        1 aataaaaaaa ttgagaattt tataatatat ttgtagattt gagtataaaa aagtgctaag
       61 ttgaaaggag taaaatatgg ataaggttaa tttagaaagg tatagttgtg atataaatgt
      121 tggtttaaat aaaaatcaac ttcaaagtag aatagatgat aatttaatta atgcatttga
      181 taatggaaaa accaaaacat ataaacaaat ttttaaagac aatatattta cactttttaa
      241 tctcataaat attgttttag tctgtttatt agtttcagtt ggttctttta aaaatacctt
      301 atttatattt attgctgtaa ttaatactat tataggagta atacaagaga taagagctaa
      361 aagattattg gacaatctat ctgttatagt ttcaaataag gtatctgtca taagagaaaa
      421 agagaaaaaa gagattgatg tacaagaatt agtattagat gatattatga ttttaaaaac
      481 aggtaatcaa atttgtgcag attcaaaagt tttaaatgga aaattagagg taaatgagtc
      541 tttggttaca ggtgaatctg atattattat aaaaaataaa ggcgattttt tatattctgg
      601 aagttttgta gtttctggag aagcttttgt aagagttgaa aatataggaa aagataacta
      661 tgcaaataag atagctaatg atgcaaagat ttctaaaaaa cataattctc aacttagaaa
      721 ttcattaaat gcaatattaa aaattgtagg tataataata attcctttag gaattatatt
      781 gtttgcaaaa caacattttg gaaatggaga ttctatagct acatctgtag taggtacagt
      841 ggcagctgta aatggtatga taccagaggg attaacatta cttacgagca ttgctctagc
      901 tgtaggaact ataaaacttg ctagtcataa aactttagtg caagaactgt actgtgtaga
      961 gactcttgct agagttgata cactatgtct tgataagaca ggtacaatta cagaaggtaa
     1021 aatgcaagtt gatgatatag taatgttgga agaagtggat attaatgaaa taatggggaa
     1081 tatatgtaat tctctcaaag atgataatgc aacactaaat gcaataagag ataagtataa
     1141 tgttttagat acgtataaag ctaagaattt aataccattt tcttcagaaa gaaaatacag
     1201 tggggttacc tttgaaaata aaggtacata tttattaggt gcatttgaat ttatatttaa
     1261 ggaaaaaaat aaaaaactta ggatagaagt tgaaagatat tctaaaaaag gaaacagagt
     1321 aatagtttta gctcatagtg attcttatat ggaagataat aatattcctg aagatataaa
     1381 gccactagct tttatattga tattggataa aattagagat gaagctaagg aaactttaga
     1441 atttttttac aatgaaggtg tagatataaa aattatatct ggtgacaatc ctataacagt
     1501 aagtgaggta gctagaaaag caggtcttaa aacagctagt aattttattg atgcaacaac
     1561 acttaaaaat gatagtgata tatacaaagc agttgataaa tacagtgttt ttggaagggt
     1621 aagcccacaa cagaaaaaac aaatgatttt agcattaaaa aaacaaaatc atacagtagc
     1681 tatgactgga gatggtgtaa atgatgtgct agcacttaaa gaagcggatt gtagcattgc
     1741 catggcatct ggaagtgatg ttactaagaa tgtgtctaat ttagtcttat tagattcaaa
     1801 ctttgcatca atgccaaagg ttgttatgga gggaagaaaa gtaattaata acattcaaag
     1861 agcatcttct ttgtttttag taaagacaat tttttcattc ttcttgtcgt tattaacatt
     1921 attcttcttc agtagacagt atccatttgt tcctatacaa ttatcactca ttggggctgt
     1981 aacagtgggt ataccatcat tcttcttagc tcttgaagca aacaaaagta gaatttcagg
     2041 aaatttttta ctcaatatat taaaaaatgc tttaccagga gcactatgtg tgacagtaaa
     2101 tgtaattatt gtttacaata tagttgaata tttaggttat agttcaagtg tattttccac
     2161 aatgtgtgct atattaactg gtgtatgtgg tcttttaata ttaagacaac aatgtttgcc
     2221 atttaataaa gaaagattat ttttaatagt aagtatgact attgcatttg ttattgggat
     2281 tctattttta ggtggattat tctcatttgt agcattgagt agagaattaa ttctcataac
     2341 tatggcatta gcaatattaa tgccaattat gataaaagtt atgttatttg tcttagatga
     2401 gatattagag acaattgttc cagatttaaa ttaattttaa aatgatataa ataagagcag
     2461 ttttagttga actgctcttt ttagatgtgt ttaattttat gtaagaatat gtatttttat
     2521 taatcagcta tatatgagca acagtagtca acaacttcat taacctttaa gtcaaaactt
     2581 gaatcactag gaacattaaa tttttgccct tctttataag ttacaaactc attgcttcca
     2641 ggcagtttta catccatact tccagcaagc atctccatta tttcttcttc tctagtagaa
     2701 aaagtgtatt ctcctggcaa cattaatcct aaagtttttc tttctccatt aggcagtatt
     2761 attactctgc tacttacctt accatcaaaa taaacgtttg ccttctttac tgcagtaaca
     2821 ttcttaaatt cactcattag ataaaacctc caaaattatt tgtacatata tattatataa
     2881 aaataatgta aaaaatgcta tatatttaca aaaaaattta gtatttatta aaaaaattaa
     2941 taagaataaa aaagagatga aattgcttaa attgataaaa aatataaaat aaattgcaaa
     3001 gtaagcaaga aaagaataag ttgaagagtt atatattata aatattaaaa attttagtat
     3061 taataaaaaa gatattacat taatatgaaa atataggaga ttagaagtgt ttgataatta
     3121 cagtacatgt tagatatatc attgtaaatt aacatagctt tgaatataat aatgttcaga
     3181 aggcaatatt tttgaggtct gaatccaata ataatatagt cttatgattg tagatatcaa
     3241 aaaatgtaag ttactaggaa tacgttttgt agatttatat atgattaata actaaagcta
     3301 taaataaaca aaataaaaag agtatgtttc aacctatagc ttttagattt aaaatatact
     3361 cttttattaa ttatagttgt ataaattaac cataatatat aattaaaatt tttattataa
     3421 ttatatatta ggttcttcaa ttagttttcc acatacatat ctatgaatga tatttctatc
     3481 atcaccaaca tatataaatc tttcaagtct ttcaagtata gaataattgt catgatttaa
     3541 atttgaatca tcaataatca atgcatcaaa atcataacct tcttcaaagc taccaacttt
     3601 tccaaagaaa cttccaccac ttttggttgc aatataaaaa gcctcagata aagataagaa
     3661 attatacttc ttaccagagt taacccaata taatttagat aattgtattg cagataccat
     3721 tgctttaaat attgacaatg tatgtccacc acttatatct gatccaagtg ctaatctaat
     3781 attattatcg attaattttc tgataggcat agcaccacta ccaacattaa agttagaagc
     3841 tggacagtga actgccataa cattattttc tctcattaaa tctatttcat cctgagaaca
     3901 atgaacacaa tgagccatta aagtaggagt ttgaccaaat aaattatatc tattatatgc
     3961 atctccatag aatttagatt caggttctag acttctaacc cattcaatct catcataatt
     4021 ttcagataag tgtgattgaa taggaatatt atattttaaa cataaatctc ctaaaccttt
     4081 taaaagttca cttgtacagc ttggtataaa tctaggtgtt attattggtt ttacaataga
     4141 atctgtatca ctatacttat ttataatttc ttctgtatca gtaatagaat cctgagtgtt
     4201 ttctaataaa gtatcagggc aattcatatt catatttact ttaccaacgt atgctccaag
     4261 accaacttct ttgaatatat ccataagttt catagaggct tctttatgta ctgttgcaaa
     4321 cactgctatt cttgtagttc cagatttcca aagttctttt acaaattttt tatacatttt
     4381 tgttgcatat tctatatctt tataatttga ttcttctgga aatgtataag tttcaagcca
     4441 tggcaaaagc tctttatcca tagccattcc aagattttta aattgagagg catgggcatg
     4501 gaggtcattc ataccaggta ttataagctt atcagtgtaa tctactactt ttaggttttt
     4561 gtattcctta ggtatctctt taaatattcc ctttacttta cctttttcca gcataatata
     4621 actattttca aatactgtga aagtttctga agtttttgta aaaattatat ttccttttag
     4681 tacttttaac atataggtct ccttagtttt aaatttgttt taatatatgt aggtaaaatt
     4741 ttgttttaca aatcaaaatt ttaccacatg tattatatca aatctaaact ataaatacta
     4801 ttagataata gcaaatatta gtgaaataca gttgtttact attattatat ataactgaca
     4861 tttaagtgac atttaagaaa aatataatgc ctacttacat aaaatggaat gttatttaaa
     4921 gagaactttg attatatttt cagaagtttt tttatccata tcgtttaaaa caagaaaata
     4981 tctattcata gttattttta tattagtatg tcctaatctt tcagagatga tttttatatt
     5041 agttctagct agaagaagaa ctattagaat agataatata gtaagtattt acaaatatgt
     5101 aggtgttctt aaattgataa attattccat tttaatttta tagtttgaat tttatgatat
     5161 aataaaaata tataaatttt gcagtgagcg atatttttga taaagtaggg tttaacagtt
     5221 gcaatgtaag ggattgaggg tgtatgataa atgttatcaa ttgcactact catggttcac
     5281 tgcaaatttg agagagttgt atgtgtgtaa gtactgaaaa tacttagttt attttggggt
     5341 tttatattaa ctaagtggta tgtaaataaa taatgtgttt tatcatcata actcctgcta
     5401 aacatgtttt atattaacta tgtggtatgt aaatcttgtt aatgcaaata aacaacatag
     5461 cactattaat agttttatat taactatgcg gtatgtaaat agggtttctg ataaaatctt
     5521 caaacatgta aaatatgtgt tttatattaa ctatgaggta tgtaaatctt atacttagtt
     5581 agaactatat atcgacacaa atatgcttta tattaactat atggtatata aattgcaatt
     5641 tttattcgtt gtccaatctc tttgaaattt gttttatatt aactaagtgg tatgtaaatt
     5701 tgtaacaagt ataggtaaaa taccccaata atttatacag cattttatcc tttaaaatat
     5761 aattattttt tat
//
LOCUS       DAYAPA010000175         5756 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.180, whole genome shotgun sequence.
ACCESSION   DAYAPA010000175 DAYAPA010000000
VERSION     DAYAPA010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5756
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.180"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            93..788
                     /gene="cas6"
                     /locus_tag="ACGI59_003555"
     CDS             93..788
                     /gene="cas6"
                     /locus_tag="ACGI59_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HFG9741307.1"
                     /translation="MIKCLEMSVKVYLLENISLDNTSKEIRKLIDDTLIIEEKYKEFH
                     NKAGFKNYSFNLLYPLEKDKVYKKDCIYTFIIRTVDSELGMYFMNNLKNQHSDKIKVI
                     TMSKKVIPKKHIESIYSVTPIIAKFEDGYWRSNKTVEDLEKRIRENLIKKYNEFTNSK
                     IDEDFELFQVIKFINRKPISTKYKNISLLGDKISFKVSENDMAQEIANFALGVGVGEI
                     NSRGFGFVNYRWL"
     gene            800..2539
                     /locus_tag="ACGI59_003556"
     CDS             800..2539
                     /locus_tag="ACGI59_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cse4"
                     /protein_id="HFG9741308.1"
                     /translation="MLKDCLEIFEKEYKSLGNRLITDSYVLSYGTYILVKNFSNIDIL
                     EVSKNNVDSTEDIYHKFCELDYLSKLVSIDKSVDSKKIIHSNNYLSFFVKKENLRPDK
                     NNNVKLTEEIIDNYYDALLNPRIKYKSDKRKKEIYDSIENKYGKVDENKLNKIRLWIK
                     TNIFDLVESVKKDKNYLKIFFDEKIELYNDENQKYLYPNIYNKNDFNVIIDGKNYGLP
                     NDNMQLNEKKPFLKLKTRIRNSTPYLISDDEVLLQKAFFDYLTNKAANGEEVIYISSE
                     KGIHRKTDIPNEFSGYILRIKKGKELEIHDFDTVVNLNTKIKRINVECIIPIEYERHN
                     LKPNLEIGYINNLIKLEKTIDTVFFNKFMCNSYFKEIKNINLSDLVLRDILIRHREGL
                     YTWFYKGRDSIVKQTFSNYSMGIIENSIKNNNYIRAKEQYNLRIAIINYFHGGSNMAD
                     KIKVISESLFEKINSIKTKCIQSDEEYYFAVGQLASYFISKNKSKNKNHSLINPILNA
                     QNRKKIMLEIEKLFKKYNYDISYDNNRRFKNLYSMIVGYEVEEKKVNRDLLIGGYLYY
                     NLIYTKSEENENE"
     gene            2532..3521
                     /locus_tag="ACGI59_003557"
     CDS             2532..3521
                     /locus_tag="ACGI59_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006441123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I CRISPR-associated protein Cas7"
                     /protein_id="HFG9741309.1"
                     /translation="MNRRVYGVLGIKSIMSNWNADFSGYPKTTSTGEVFGSDKALKFP
                     MKKMWENEGEKVLYVKSLKIDESKKGEIKIRPKSLKERYEELFDIEDLKLEKNQQKVL
                     ENLFKAIDVKNFGATFAEEGNNIGIMGAVQIGQGFNKYEDINVEEQQILSPFRDPKAK
                     DKKKSTNEEEKEDANSTTLGKKIVSDEAHYFYPFSINPKAYEVFVNMGVTDGYTKNDY
                     KKFKEAALVSATAFNTNSKLGCENEFALFVETDEELYLPDLAQYITFEKREKSKDLIC
                     INIIHLIAELKDDIKSVEIYYNPYKIEIKPFKDEILPKIDKVKFMNIFTRKEV"
     gene            3523..4263
                     /gene="cas5b"
                     /locus_tag="ACGI59_003558"
     CDS             3523..4263
                     /gene="cas5b"
                     /locus_tag="ACGI59_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein Cas5b"
                     /protein_id="HFG9741310.1"
                     /translation="MEVLKFNLSGKTAFFKKPDVNSIIYFTYGNIHKIALMGILGACL
                     GLGGYNQQSKEDEYPEFYSLLKDIKMSIVPKHEFGYINKKVHIFNNSVGYASKEEGGN
                     LIIKEQWLENVSWDIYIQLEDSTTSRELKRRFLSNKFVYIPYLGKNDHIANISEIEII
                     TDFYKTEDINRVDSFFIREDFEFEKSYFDDNDDYKEPFKYQEKLPYRLHRMTNQYILE
                     TIIFTNMSVKLINKKEVYQVKGLNLIFI"
     gene            4287..>5756
                     /locus_tag="ACGI59_003559"
     CDS             4287..>5756
                     /locus_tag="ACGI59_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
                     /protein_id="HFG9741311.1"
                     /translation="MYFKKVELFDISNNINNCKNIYAHINKDRKPETLKEHSDLSYSY
                     LMKLCVKKDLSSCLERIENKFFKKDSFLSKNFYREIILNMVYMHDIGKINSSFQFVKM
                     QNKNFKKISLRYTKHSFISSMLYFDYYFEKLKKYDFEEKKILSLFLMINTYTISKHHG
                     VLKSFKEFLFLFKDEFINIKENFKDLFKNNYNLELKSVPKTIEKMVRGVEAYLDDSHI
                     KDNLAIDLYIYSKLMFSIVTSCDFYATSEYNSGNSIDDFGTIDNYNKYYDVLKKSEIY
                     KGMKKYREYLNNNSECPYKENDINRLRTEMSIEAEDAITHNTRKRIFYLEAPTGSGKT
                     VTSINLALKSIELNQSLKKIFYIFPFNTLVEQTKDVFINEIFNSVKDIQKDVVVINSI
                     TPIQTEDKEEDNKNVEYCVTKQEIDYDKSLLNRQFLHYPIVLTTNIGFFNYLFGVSRE
                     ECFPLIHMINSVIILDEIQNYKNEIWKEIILFLEKYADIL"
BASE COUNT         2526 a          480 c          880 g         1870 t
ORIGIN      
        1 tttataacta taaattatat tttagagttt attgttatta aatagatgaa aaaatcctaa
       61 tatggattat cagaaaatga ggaggttgtt aattgataaa atgtcttgaa atgtctgtaa
      121 aagtgtatct tcttgaaaat atttctttag ataatacatc aaaagaaata cgtaaattaa
      181 tagatgatac attaattata gaagaaaaat ataaggaatt tcataataaa gctggattta
      241 agaattattc ttttaattta ttatacccat tagaaaaaga taaggtttat aagaaggatt
      301 gtatatatac ttttattatt agaactgtag atagtgaatt aggaatgtat tttatgaata
      361 atttaaaaaa tcagcattct gataaaataa aggttattac aatgagtaaa aaagtgatcc
      421 ctaaaaaaca tatagaaagt atttactctg tcactccaat aatagctaaa tttgaggatg
      481 ggtattggag aagtaataaa acagtagaag atttagaaaa aagaatcaga gaaaatttaa
      541 taaaaaaata taatgaattt actaacagta aaatagatga agattttgaa ttatttcaag
      601 ttattaaatt tataaatagg aaacctattt ctactaaata taagaatata tctttacttg
      661 gtgataaaat aagttttaaa gtatcagaaa atgatatggc tcaggaaatt gcaaattttg
      721 cacttggagt tggagtcgga gaaattaatt ctcgtggatt tggttttgta aactatagat
      781 ggttatagga ggtgaaaact tgttaaagga ttgtttagag atttttgaaa aagaatacaa
      841 atctttagga aatagattga taactgatag ctatgtccta tcctatggaa catatatatt
      901 ggtgaaaaat ttttcgaata tagatatatt agaagtttct aaaaataatg tggatagtac
      961 agaagatata taccataaat tttgtgaact ggattattta tcaaaactgg tgtctataga
     1021 taaatcagtt gattccaaaa aaataataca tagtaataat tatctttcat tttttgtaaa
     1081 aaaggaaaac ttaagacctg ataaaaataa taatgtaaaa cttacagaag aaattattga
     1141 taattattat gatgctttgt taaacccaag aattaaatat aaatctgata aaagaaaaaa
     1201 agaaatctat gattcaatag aaaataaata tggtaaagtg gatgaaaata aattaaataa
     1261 aataagacta tggataaaaa caaatatatt tgatttagtt gaatctgtta aaaaagataa
     1321 aaattattta aaaatatttt ttgatgaaaa aatagagtta tacaatgatg aaaaccaaaa
     1381 atatttatat ccaaatatat ataataaaaa tgatttcaat gtaattattg atggtaaaaa
     1441 ctatgggtta ccaaacgata atatgcaatt aaatgaaaaa aagccatttt tgaaattaaa
     1501 aacaagaatt agaaattcaa ctccttattt gattagtgat gatgaagttt tgttacagaa
     1561 agcttttttt gattatctaa cgaataaagc agcgaatgga gaagaagtaa tatatataag
     1621 tagcgaaaag ggaattcata gaaaaactga tataccaaat gaatttagtg gttatatact
     1681 gagaataaaa aagggtaaag aattagaaat acatgatttt gatactgtgg taaatttaaa
     1741 cactaaaata aaaagaataa atgtagaatg tataatacca atagaatatg aaagacataa
     1801 tttaaaacca aatttagaga taggatatat taataatctt ataaaattgg aaaaaacgat
     1861 tgatactgta ttctttaata aatttatgtg caatagttat tttaaagaaa ttaaaaatat
     1921 aaacttaagt gatttagttc taagggatat acttattaga catagagaag gtttatatac
     1981 ctggttttat aaagggagag atagtatagt aaagcaaact ttttcaaact attctatggg
     2041 tataattgaa aattctataa aaaacaataa ctacataaga gcaaaggagc aatataattt
     2101 aagaatagct ataataaatt attttcatgg aggaagtaat atggcagata aaataaaagt
     2161 tattagtgaa tctttatttg aaaaaataaa ttctataaaa acgaaatgta tacaaagtga
     2221 tgaagaatat tattttgcag ttgggcaatt agcatcgtat tttatatcta aaaataagtc
     2281 aaaaaataaa aatcattctc tgattaatcc tatactaaat gcacaaaata gaaaaaaaat
     2341 catgttagag atagaaaaat tatttaaaaa atataattat gatataagtt atgacaacaa
     2401 taggcgattt aagaatctat attctatgat tgtaggatat gaggtagaag aaaaaaaagt
     2461 taatagagat ttattaattg gtggatatct atattacaat ttaatataca caaaaagtga
     2521 ggagaatgaa aatgaataga agagtatatg gagtattagg tataaaatct ataatgtcaa
     2581 actggaatgc tgatttttct ggatatccaa agacgacatc aacaggagaa gtttttggga
     2641 gtgataaagc acttaagttt cccatgaaaa aaatgtggga aaatgaaggt gaaaaagttt
     2701 tatatgttaa atcattaaaa atagatgaat ctaagaaagg agaaataaaa attagaccaa
     2761 aatcattaaa agagagatat gaagaattgt ttgatataga ggacctaaaa ctagaaaaga
     2821 atcaacaaaa agtactggaa aatctattta aagctattga tgttaaaaat tttggtgcaa
     2881 catttgcaga agaaggaaat aatataggaa ttatgggcgc agtacaaatt ggacaaggat
     2941 ttaataagta tgaagatatt aatgttgaag aacaacaaat attatcccca tttagggatc
     3001 caaaagcaaa agataagaaa aagtcaacaa atgaagaaga aaaagaagat gcaaattcaa
     3061 ctacattagg aaaaaaaata gtaagtgatg aggctcatta cttttatcca ttttcaataa
     3121 atccaaaagc ttatgaagtt tttgttaata tgggtgtgac tgatggatat actaaaaatg
     3181 attataaaaa atttaaagaa gctgctttag taagtgcaac tgcattcaat acaaattcaa
     3241 agcttggttg cgaaaatgaa tttgcattat ttgttgaaac tgatgaagag ttatacctac
     3301 cagatttggc acaatatata acttttgaaa agagagaaaa atcaaaagat ttaatttgta
     3361 taaatataat tcatcttata gcagaattaa aagatgatat aaagagcgtt gagatatatt
     3421 ataacccata taaaatagaa ataaagcctt ttaaagatga aatattacct aagatagata
     3481 aagttaaatt tatgaacata tttacaagaa aagaggtata atatggaggt tttgaaattt
     3541 aacttaagtg gaaaaactgc tttttttaaa aaaccagatg taaatagcat aatttatttt
     3601 acgtatggaa acatacataa aattgcactt atgggtattt taggagcttg tttaggactt
     3661 ggtggatata atcaacagag taaggaagat gagtatccag aattctactc tttacttaag
     3721 gatataaaaa tgtcaatagt tccaaagcat gaatttggat atataaataa aaaagtccat
     3781 atatttaata attctgtagg atatgcatca aaagaagaag gtggaaattt aataataaaa
     3841 gaacagtggc ttgaaaatgt tagttgggat atatatatac aattagaaga ttcaactaca
     3901 agtagagagc ttaaaagaag atttttatct aataaatttg tatatatacc ttacttaggc
     3961 aaaaacgatc atatagctaa tataagtgaa atagagataa ttactgattt ttacaaaaca
     4021 gaagatatta acagagtgga cagtttcttt ataagagagg attttgagtt tgaaaaatct
     4081 tattttgatg ataatgatga ttataaagag ccatttaaat atcaggaaaa acttccttat
     4141 agactgcacc gaatgactaa tcaatatatt ttggaaacta tcatatttac taatatgtca
     4201 gttaaattga ttaataaaaa agaggtatat caagtaaagg gtttaaattt aatctttatt
     4261 taaatttata ggtttaggag aattatatgt actttaaaaa agttgagttg tttgatataa
     4321 gtaataatat aaataattgc aaaaatattt atgctcatat aaataaagac aggaagccag
     4381 aaactttaaa agaacattca gacttatcat actcatatct tatgaaatta tgtgtaaaaa
     4441 aagatttaag ctcttgccta gagagaatag agaataaatt ttttaaaaaa gattcttttt
     4501 taagtaagaa tttttataga gagattatct tgaatatggt ttatatgcat gatataggta
     4561 agattaattc ttcatttcag tttgtaaaaa tgcaaaataa aaattttaaa aaaatttcac
     4621 ttagatatac taaacattct tttatctctt caatgctata ttttgattac tattttgaaa
     4681 agcttaaaaa atatgatttt gaagaaaaaa aaatattgag tttatttttg atgataaata
     4741 catatacaat atcaaaacat catggggttc tgaagtcatt taaagaattt ttatttttat
     4801 ttaaagatga gtttataaac attaaagaaa attttaaaga tttatttaaa aacaattata
     4861 atttagagct taaatcagtt cccaaaacta tagaaaaaat ggtaagaggt gtagaagcgt
     4921 atttagatga tagccatata aaggataact tagctataga cttatatata tattcaaaat
     4981 tgatgttttc aattgttaca tcttgtgatt tttatgctac atcagaatat aatagtggta
     5041 attctataga tgattttggg acaattgata attataataa gtattatgat gtattaaaaa
     5101 aatctgaaat atataagggg atgaaaaaat atagggaata cttaaataac aatagtgaat
     5161 gcccatataa agagaatgat ataaacagat tgagaacaga aatgagtata gaagctgaag
     5221 atgcaattac acataatacg agaaaaagaa tattttattt agaagcacca actggaagtg
     5281 gaaaaactgt tacatctata aatctagcac tgaaatctat agagttaaat caatctttaa
     5341 aaaagatatt ttatatattt ccatttaaca cattagtaga gcaaacaaaa gatgtattta
     5401 taaatgaaat atttaattct gttaaagaca tacaaaaaga tgttgtagtt ataaattcaa
     5461 taacaccaat acaaacagaa gataaagaag aagataataa aaatgttgaa tattgtgtta
     5521 ctaaacaaga aattgattat gataaatcat tgttaaatag acaattttta cactatccta
     5581 tagtacttac aactaatata ggttttttta actatttgtt tggtgtatca agagaagaat
     5641 gttttccatt gatacatatg ataaatagtg taattatatt agatgaaata caaaattata
     5701 aaaatgagat atggaaagaa attattttgt ttttagaaaa atatgcagat atatta
//
LOCUS       DAYAPA010000176         5714 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.181, whole genome shotgun sequence.
ACCESSION   DAYAPA010000176 DAYAPA010000000
VERSION     DAYAPA010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5714
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.181"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            <1..1046
                     /locus_tag="ACGI59_003560"
     CDS             <1..1046
                     /locus_tag="ACGI59_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HFG9741312.1"
                     /translation="KLGCNDDNFAYLVKNREKFFKNHLFKDRVNISFEMLNSEINDIE
                     EISEKVIEEAEIYNKILIEFIKKSSALKFYKNIKEKLKYKLEDVFLLTGDDNKLERKK
                     IINKIKDMNSVILVATQVVEAGVDIDMDLGFKDISTIDSDEQFLGRINRSCKKLNSKV
                     YFFNLDDASKIYKKDVRKERHLTLNEESNRKIILDKDFEKYYDKIIARIEENKHKHND
                     KNIEVFIKDIVGNLNFTEVKKWMALIPDLKEYTIFLNTIVKDESGNMIVGSDVWYEYI
                     SLLKNDNVEYSEKRVKMSEIMEKLDYFTYKVQKFDNSFNDLVGDIFYIDDGSKYFTEG
                     KFDRSKFNQNEFL"
     repeat_region   1236..4104
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=1236..1265
                     /rpt_unit_seq="gttaaacagtaacatgagatgtatttaaat"
     gene            4189..4683
                     /locus_tag="ACGI59_003561"
     CDS             4189..4683
                     /locus_tag="ACGI59_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HFG9741313.1"
                     /translation="MKITGTLINYYFHCKRQCWLLGNRINLEENSEDVKIGRLLHELK
                     EEKTKYKEIAIENIKIDKLTREYLVEVKKSDADIEAVKWQVLLYLKKLKEKGIVRKGK
                     IEFIEKNKTNKKIVFVDLSEVSEEQLISIEREIVNLINSDKAPCPDLKKGCKKCAYYE
                     YCYI"
     gene            4718..>5714
                     /gene="cas1b"
                     /locus_tag="ACGI59_003562"
     CDS             4718..>5714
                     /gene="cas1b"
                     /locus_tag="ACGI59_003562"
                     /inference="COORDINATES: protein motif:HMM:TIGR03641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated endonuclease Cas1b"
                     /protein_id="HFG9741314.1"
                     /translation="MGKTKYITSMGELTRKDNSICFRKNGRNVYIPVENTSEIFCLNE
                     VSLNSKLLDFLSRANIVVHFFNYYGGYSGTFYPKEYLISGKVTVAQVKTFEDRREEIA
                     KSIVNGIRINAINTLSHYYKHGNSEIQPLLSHLKKDSINKLEKSNNIKEILSVEGDIW
                     QTFYTSFKYILREEFIFNKRVKRPPDNPLNAMISFGNSILYAKTVTAIYNTHLNQSVS
                     FLHEPSESRFSLSLDLSENFKHVITFKTIFDLVNNRKINIEKHFDKNLNYCVLNDEGK
                     QIFINSLEERLESVKEHPKLKRKVSLKTCIKLDAYKMLKCIMEEKSFIPYNIKDGL"
BASE COUNT         2391 a          553 c          882 g         1888 t
ORIGIN      
        1 ataaattagg atgtaatgat gataattttg catatctagt aaaaaacaga gaaaaatttt
       61 ttaaaaatca cctttttaaa gatagagtaa acataagttt tgagatgtta aattcagaaa
      121 ttaatgatat tgaagaaata tcagaaaagg ttatagaaga agcagaaata tataataaga
      181 tactgataga gtttataaag aaatcatcag cactaaaatt ttataaaaat attaaagaaa
      241 aattgaagta taagttagaa gatgtttttt tattaacagg tgatgataat aaattagaaa
      301 gaaaaaaaat aataaataaa attaaagata tgaactctgt aattttggtt gctacacaag
      361 ttgtagaggc tggagtagat atagatatgg atttgggatt taaagatata tctacaattg
      421 attctgatga acaatttttg ggaagaataa atagatcttg taaaaaatta aattcaaaag
      481 tatacttttt taatttagat gatgcaagta agatatataa gaaagatgtt agaaaagaga
      541 gacatttaac tcttaatgag gaaagtaata gaaaaattat tttagataaa gactttgaaa
      601 agtattatga taaaataata gccagaatag aagaaaataa gcataaacat aatgacaaga
      661 atatagaggt ttttataaaa gatattgtgg gaaatttgaa ttttacagag gtaaaaaaat
      721 ggatggcttt aataccagat ttaaaagagt atacaatctt tttaaatact atagttaaag
      781 atgaatctgg aaatatgatt gtaggaagtg atgtatggta tgaatatata tcactactaa
      841 aaaatgataa tgttgagtat tctgaaaaga gggtgaaaat gtctgaaatt atggaaaaac
      901 tagattattt tacgtataaa gttcagaaat ttgataattc atttaatgat ttagttggag
      961 atatttttta tattgatgat ggaagtaagt attttacaga gggaaagttt gatagaagta
     1021 aatttaatca aaatgaattt ctataatgta tttagattat atagagaata tttatcccaa
     1081 gttaattaat tagaattagt ttaaaactat tgaaatatag atattttata gcttatatat
     1141 tttttctagg attagctggg ataaaaaatc tgtatattag tgttttcagt gtatttaagg
     1201 ggattatatt tttaagaagt actgatttta aagtggttaa acagtaacat gagatgtatt
     1261 taaattttta ctacataatt aaatcgtagt caataccgta caggttaaac agtaacatga
     1321 gatgtattta aatctctgaa tagccaatca actttgctgc attactcttg ttaaacagta
     1381 acatgagatg tatttaaatg ataatagtta tatatatttt ttacaatata ttattgttaa
     1441 acagtaacat gagatgtatt taaatttact tctcctgttt tgaaatctat atattcatca
     1501 tgttaaacag taacatgaga tgtatttaaa tataatattg atacaaatat tttgggtaaa
     1561 tggacttgtt aaacagtaac atgagatgta tttaaataaa gaaaaacgaa attggtgatt
     1621 ttgaagcaat gtgttaaaca gtaacatgag atgtatttaa attagtaact acaatgcttt
     1681 tccttttgaa gatgatatgt taaacagtaa catgagatgt atttaaatta ataatgcacc
     1741 tattttaaca gtacctggtc taaagttaaa cagtaacatg agatgtattt aaattcaagt
     1801 ctaggagaca acataaatag tacagatggg ttaaacagta acatgagatg tatttaaatt
     1861 agtaaaacca gatggaactg ttgtaatacc accaaagtta aacagtaaca tgagatgtat
     1921 ttaaatttaa aaacatcttg aggaatatct ttaacagtaa ttgttaaaca gtaacatgag
     1981 atgtatttaa atgcattttc aggtgcatga taaataggta aattagatgt taaacagtaa
     2041 catgagatgt atttaaattt tttgtaatta gccagattat caactcccaa aatagttaaa
     2101 cagtaacatg agatgtattt aaattaaata tcgtccttcg tagtcaaagc aacaccagaa
     2161 ggttaaacag taacatgaga tgtatttaaa tcgtaaacaa aatttatcaa gagaagaatt
     2221 tctaaggtta aacagtaaca tgagatgtat ttaaatttat atttgataag agtatcaaat
     2281 cgacttaaaa tagttaaaca gtaacatgag atgtatttaa attcaatctt aatacacaga
     2341 catttttaaa aacaaaacgt taaacagtaa catgagatgt atttaaataa atagacaaat
     2401 attgaatagc tctgtcttca tcaagttaaa cagtaacatg agatgtattt aaatatattt
     2461 acaaaatctc taacatattt tttaacttct gttaaacagt aacatgagat gtatttaaat
     2521 tcttttaaag agtcctcggg agcctcgaaa acttcagtta aacagtaaca tgagatgtat
     2581 ttaaattact caaataaatt tctaatttgt ttatctacta acgttaaaca gtaacatgag
     2641 atgtatttaa attagaattt acaacaatat aagcctcttt aaccataagt taaacagtaa
     2701 catgagatgt atttaaatat gtttttagta gtacatttaa taaaactttt taaagttaaa
     2761 cagtaacatg agatgtattt aaatgaagga accgtacctt gataaatcac gaccgtaccc
     2821 gttaaacagt aacatgagat gtatttaaat tgtatatttg atgttgttat ttctcctgtg
     2881 tataatgtta aacagtaaca tgagatgtat ttaaatgaag ttccaccccc accaccccca
     2941 ctactttgag aagttaaaca gtaacatgag atgtatttaa atcccctttt gcatggtact
     3001 agacacgcat atatggtggt taaacagtaa catgagatgt atttaaatac gcctatatgc
     3061 ggagtaacag atttttgcct ggatggttaa acagtaacat gagatgtatt taaatactgt
     3121 tttgggatag actggaatta aatggtctgt gttaaacagt aacatgagat gtatttaaat
     3181 ttttggaatg aagcatcaac agataataaa gaaaaagtta aacagtaaca tgagatgtat
     3241 ttaaatcaag gaaacaaaag agaagtgaaa agaaatataa tgttaaacag taacatgaga
     3301 tgtatttaaa taatctttta aatggaaatt aagtaataaa agagagggtt aaacagtaac
     3361 atgagatgta tttaaattca attaaataag cattaatctt agtaaaatca aacgttaaac
     3421 agtaacatga gatgtattta aattttcttg tgttaattga acccccattt taagctccag
     3481 ttaaacagta acatgagatg tatttaaatc agaatacagc ttctatatct ggatacattt
     3541 gtcttggtta aacagtaaca tgagatgtat ttaaatgtga aaagtaatac aggtatagag
     3601 tctaaacgct cggttaaaca gtaacatgag atgtatttaa atcaatgtat cctctttttt
     3661 agttttggag cctcttttgt taaacagtaa catgagatgt atttaaatta gttagcacat
     3721 tttatttcaa gccttaaaac attcgttaaa cagtaacatg agatgtattt aaattaattt
     3781 gcacacttaa cctctaatct taaagtatta gttaaacagt aacatgagat gtatttaaat
     3841 aacacaatat tatctttaat agaattaaac aaatgagtta aacagtaaca tgagatgtat
     3901 ttaaattttt gttggtcttg tgcttctttt aaaagctgat tagttaaaca gtaacatgag
     3961 atgtatttaa atttaaaaag ttctgttatt aaaacatcca aaccaatcgt taaacagtaa
     4021 catgagatgt atttaaattc tgtaccatct agccaaaccg tttttgaagt taaagttaaa
     4081 cagtaacata agatgtatct aaattactct atcataaaat tttaatatat taaacaaact
     4141 aatatgtgat atacttatgt ataaataaca aaataaatag gtgatgttat gaaaattaca
     4201 ggaactttga taaattacta tttccattgt aaaagacaat gctggttgct tggtaataga
     4261 ataaatttgg aagaaaatag tgaagatgta aaaataggta gacttcttca tgaactaaaa
     4321 gaagaaaaaa caaaatataa agaaattgca attgaaaata taaaaataga caaattaaca
     4381 agagaatatt tagtagaggt taaaaagtct gatgctgata tagaagctgt taagtggcaa
     4441 gttcttttat atttaaaaaa gttaaaagaa aagggaattg taagaaaagg gaaaattgaa
     4501 tttatagaaa aaaataaaac taataaaaaa atagtctttg tagatttgag tgaagtatct
     4561 gaagaacagc ttatatctat agaaagagaa attgttaatt taataaatag tgataaagca
     4621 ccatgtcctg atttaaaaaa aggatgtaaa aaatgtgcct attatgaata ttgttatata
     4681 taaagaggta taagaaacat ataaggaggt gttaatattg ggaaagacaa aatatattac
     4741 ctcaatgggt gaactcacaa gaaaagataa ttctatatgt tttagaaaaa atggaaggaa
     4801 tgtatatata ccagtagaaa atactagtga aatattctgt ctaaatgagg tatctttaaa
     4861 ttcaaaatta ctggattttt tatcaagagc aaatattgtt gttcattttt ttaattacta
     4921 tggtggatat agtggaactt tttatcctaa agaatattta ataagtggaa aagtaacagt
     4981 ggcacaggta aaaacctttg aagatagaag agaagaaata gcaaaatcca tagtcaatgg
     5041 tataaggatt aatgcaataa atacattatc gcattactat aagcatggaa attcagaaat
     5101 acaaccatta ttatcacatc taaaaaaaga ttctataaac aagttagaaa aatcaaataa
     5161 tataaaagaa atattatcag tcgaaggaga tatttggcaa acattttata cttcctttaa
     5221 gtatatttta agagaggaat ttatttttaa taaaagagtt aagagaccac cagataatcc
     5281 cttaaacgca atgatatcat ttggtaacag tattttatat gctaaaactg taactgctat
     5341 atataataca catctaaatc aaagtgtaag ttttttacat gaaccaagtg aaagtagatt
     5401 ttctttaagt ctagatttaa gtgaaaattt taaacatgtt ataactttta aaactatatt
     5461 tgatttagtt aataatagaa aaataaatat tgaaaaacat tttgataaaa atttgaacta
     5521 ctgtgttcta aatgatgaag gaaagcaaat ttttataaat tctttagaag aaagattaga
     5581 atcagtcaaa gaacatccaa aattaaaacg aaaagtaagt ttaaaaacct gtataaaatt
     5641 agatgcttac aaaatgttaa agtgtataat ggaagaaaaa agttttattc catataacat
     5701 aaaagacgga ttat
//
LOCUS       DAYAPA010000177         5697 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.182, whole genome shotgun sequence.
ACCESSION   DAYAPA010000177 DAYAPA010000000
VERSION     DAYAPA010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5697
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.182"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(555..1106)
                     /locus_tag="ACGI59_003563"
     CDS             complement(555..1106)
                     /locus_tag="ACGI59_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFG9741315.1"
                     /translation="MSINTVIAKNLNRLRNERNLSLGQLAELSGVSKVMLSQIEKGDS
                     NPTVNTIWKIASGLNVPYTAILEQPQNETFIVSKTDIDVQVSENKDYRLYCYYPNTPT
                     RNFELFQMELEEGHSYTSVGHSEKSQEYIMIIEGQLKLEVNDSIYQLRENDSICFSAE
                     SIHTYHNQGEKTLKAVIINYYPV"
     gene            1297..2259
                     /locus_tag="ACGI59_003564"
     CDS             1297..2259
                     /locus_tag="ACGI59_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HFG9741316.1"
                     /translation="MRVLNNVTEAIGNTHMLHLSKIADEFGVQGNIYAKMEYLNPGFS
                     KKDRVALQMITEAENAGLLVPGQAVVELTSGNTGTGLAIACACKGYKFIACMSKGNSM
                     ERARMMKALGAEVVLVDQMPNSVHGQVSGEDLALVEIEAQKIAKERNAFRADQFNLEA
                     CNHAHEKYTAEEMWEQLDGNIDVFVDFLGSGSTFAGCSKTLKKHNSSIKCYVVEPETA
                     AIYSGKEITDQGHKIQGGGYSMDLPLVDKKLIDGYIQVTDEEATDVARKLAKLEGVFA
                     GFSSGANVCAAIKLLKSSEKGKNIVLTLNDSGLKYLSTDLYEEL"
     gene            2554..4398
                     /locus_tag="ACGI59_003565"
     CDS             2554..4398
                     /locus_tag="ACGI59_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HFG9741317.1"
                     /translation="MQKRILRNFIFTGKNILSKLVFIIKYNFSTLILFELFHKGLALF
                     FIWPSIRYIFDILIKSLHIVYLNMDNFIKVLTNPMSIVLIILSVIILAFYAFFEFTSV
                     IICFNKSIKYEKIGLFELFKISFKNSIKLLYPKNILLFIFVILIIPLVNTVLISGFIG
                     KIQIPEYILDYIKSDIILNIIYMIFLLIIYISVIRWIFSIHEITLNTENFKRARKESV
                     NLIKGKIIRTIIYSLILSLVVAIIGYAIYHSGIIFIGIWTKYCTDMEFLKNVFINRCI
                     AFEEYFRLILSIFIFILDIGFISATYYQYKGVNISKNNIKKEKKSISKIVFNLVLILL
                     ISYIESVAFSSHINGMFNTSFSFYTTAIAHRAGAEMAPENTLAAIEEASHAKADYAEI
                     DVQETKDGELIVLHDSNFKRTTGVDKNVWDVNYNEVKTYDAGSFYYYGKGYYDEKFVG
                     EKIPTLKEMIKYSKGKVKLLIEIKLNGHEKGDVVKKVVQLIKENNFENQCIVASMDKT
                     ILQKVKKLDENLLTCYFMAIAYGDFSKLNYVDIYGIESTFVNKTVVEKIHKMNKKIFV
                     WTVNSDSLIEKMLDLNVDSIITDNPYLIESAIYWKKNGFISQVSDYLF"
     gene            4720..5304
                     /locus_tag="ACGI59_003566"
     CDS             4720..5304
                     /locus_tag="ACGI59_003566"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HFG9741318.1"
                     /translation="MTEREKMLAGELYDCGDIELLTQWHKAKGLVRDYNQTDSSDLEK
                     KEKILNELLGGKGKNLWITAPYYVDYGNNIYFGNNCEVNMNCTFLDDNKIIIGDNALI
                     APNVQIYTAFHPTNAGDRFGNAKEDGSFEFCKTQTAPVTIGDNVWIGGGAIIMPGVTI
                     GDNVVIGAGSIVTKDIPSNMIAYGNPCRIIRENK"
BASE COUNT         2215 a          564 c          890 g         2028 t
ORIGIN      
        1 ttatttaacc tcttaaatta tatttagata taggagtctt atgataaaag taagcttatg
       61 tgttatttgt agtaaatata aagtgcatat agtgataaaa caaaagtttc atataaatga
      121 agaaaaaaat ataaaataat ttatttaact acttaaaaat aaactaaaag ggataataaa
      181 atagctttta ggttatggta ataaaggtat ttaaaaagag tgcataagca ctccaattgt
      241 gagaaataca tacacaaatc attattacaa caatgaaaaa tgtacatata agagacgttt
      301 tgtatttgtt ttaagtgata atattttaat actaactaaa taattatcac aatagtaaaa
      361 aagaaaaagg agtattttgt actccatttg tcaaaaatat aaaactttta tataagctta
      421 ttataacata attgataaaa gtgtaaagat atataatgaa tttataaaga aaaataaaat
      481 aaaaaagata aatcctaatc aaaggattta tctttttgta tagaaaataa ttattctatt
      541 attaaatggc atggctaaac tggataataa tttattatta ctgctttcaa tgttttttct
      601 ccttgattat ggtatgtatg aattgattca gctgaaaaac aaatagagtc attttctcta
      661 agttgataaa tagagtcatt tacttctaat tttaattgac cttctataat cataatatat
      721 tcctgtgatt tttcagaatg accaacagat gtataactat gaccttcttc taattccatt
      781 tgaaataatt cgaagtttct agttggtgta ttgggataat aacaataaag acgataatct
      841 ttattttctg aaacttgtac atctatatca gtttttgata caataaaagt ttcattttga
      901 ggttgttcca gtattgcagt atatggaaca tttagtccac ttgcaatttt ccaaattgtg
      961 tttactgttg ggtttgagtc tcccttttca atttgcgaaa gcattacttt acttacgcca
     1021 gataattcag caagttgacc tagacttaag ttacgttcat tacgtaatct atttaaattt
     1081 ttagcaataa cagtattaat actcatagta gctccttaaa atgtaaatta ttgacaaata
     1141 taatttacga atgtataatt tatttaacat tatttataat ttacaaatgt ataatataat
     1201 gaacgttaaa tacactgtac atccgtaata taaattatac tgcataaaga gttgtttttc
     1261 aacggatgat agttgagaga ttaaggggga attgtaatgc gtgtacttaa taatgttaca
     1321 gaggctatag gcaatactca tatgttacat ttatccaaaa tagcagatga atttggagtt
     1381 caaggtaata tttatgcaaa gatggaatac ttaaatccgg gatttagtaa aaaggaccgt
     1441 gtagcattac aaatgattac cgaagcagaa aatgcaggtt tattagttcc aggtcaagca
     1501 gttgtggaat taacaagtgg taatactgga acaggactag caatagcttg tgcatgtaaa
     1561 ggatataaat ttatagcgtg tatgtcaaaa ggaaattcta tggaaagagc aagaatgatg
     1621 aaagctttag gtgctgaagt agttcttgta gaccaaatgc ctaattcagt tcatggacag
     1681 gtatctggtg aagatttggc acttgtagaa attgaggctc aaaaaatagc aaaagaaaga
     1741 aatgcattta gagcagacca atttaattta gaagcttgta atcatgcaca tgaaaagtat
     1801 acagcagaag aaatgtggga acaattggat ggaaatattg atgtatttgt agattttctt
     1861 ggaagtggta gtacttttgc aggatgttca aaaacactta aaaaacataa ttcttctatc
     1921 aaatgttatg ttgttgaacc tgaaacagca gcaatttatt caggtaaaga aataactgac
     1981 caagggcata aaatacaagg tggaggatat tctatggatt tgccattagt agacaagaaa
     2041 cttattgatg gttatattca agttacagac gaggaggcaa ctgatgtagc aagaaaatta
     2101 gctaaattag aaggtgtatt tgcaggattt tcttcagggg caaatgtatg tgcagcaatt
     2161 aagttgttaa agagcagtga aaaaggaaaa aatatagttt taactcttaa tgatagtggt
     2221 ttaaaatatt taagtactga tttatatgaa gaattgtaat aatagcacaa tagttatgca
     2281 atgtttttta aattaagtat atagatttaa attataaaaa gtatctcaat gtaatttttg
     2341 aaagttataa aggagatact tttttatttt gtaaatatgg agtatcctac ttataagtat
     2401 taagctatat agatattgat gattagctat gcaataaaaa agagtatgtg tttttgcatt
     2461 tatacatcat aacttggaat aatacatatt aatgttctaa atgtatttaa aacatatgat
     2521 aaaattagaa caaatagata caaggagata aaaatgcaaa agagaatttt aagaaatttt
     2581 atatttacgg gtaaaaatat tttaagtaaa cttgttttta taataaaata taattttagt
     2641 acattaattt tatttgaatt atttcataaa ggattagcct tattttttat atggccatct
     2701 attagatata tatttgatat tttaataaaa agtttacata tagtctacct aaatatggat
     2761 aattttataa aagttttgac caatcccatg tcaatagttc tgataatact atcagttata
     2821 atactagctt tttatgcatt ttttgaattt acctctgtaa ttatttgttt taataaatcg
     2881 ataaaatatg aaaaaatagg gttatttgaa ctctttaaaa tatcatttaa gaactctata
     2941 aaattgttat atcctaaaaa tatactatta tttatatttg tgatattaat aattccatta
     3001 gtaaatacag ttttaatttc agggtttata ggaaaaatac aaataccaga gtacatacta
     3061 gattatataa agtctgatat aattttaaat ataatctata tgatttttct tttaataata
     3121 tatatttcgg ttattagatg gatttttagc attcatgaga taactttaaa tacagaaaat
     3181 tttaaaagag caagaaaaga aagtgttaat cttataaagg gaaagattat aagaacaatt
     3241 atttattcat taatattatc tttagtagta gctattattg gatatgcgat ttatcatagt
     3301 ggaattatat ttataggcat atggactaaa tattgtactg atatggaatt tttgaagaat
     3361 gtatttataa atagatgtat tgcatttgaa gaatatttta gattgatttt atcaatattc
     3421 atttttatat tggacattgg atttatctca gcaacttatt atcaatataa aggagtaaat
     3481 atttctaaaa ataatattaa aaaagagaaa aaatctatat caaaaattgt ttttaattta
     3541 gtattaatat tattgatttc ttatatagag tcagttgcat tttcttcaca tataaatggt
     3601 atgtttaata cgtcattttc attttataca acagcaattg ctcatagagc aggagctgaa
     3661 atggctccag agaatacttt ggcagcaata gaagaagctt cacatgctaa agctgattat
     3721 gcagaaatag atgttcaaga aactaaagat ggagaactta ttgtacttca tgattcaaac
     3781 tttaagagaa ctacaggagt agataaaaat gtttgggatg taaattataa tgaagtaaag
     3841 acatatgatg cgggaagctt ttattattat ggaaaaggtt attatgatga aaagtttgtt
     3901 ggggaaaaga taccaacact caaagaaatg ataaaatatt ctaaaggtaa ggtgaaatta
     3961 ttaatagaaa taaaacttaa tggacatgaa aagggagacg tagtaaaaaa agttgttcag
     4021 ttaattaaag aaaataattt tgaaaatcaa tgtatagttg catctatgga taaaactata
     4081 ttacaaaaag taaaaaaact ggatgagaat ttattgacat gctattttat ggcaattgca
     4141 tatggtgatt tttcaaagtt aaattatgtt gatatatatg gaattgaatc aacttttgtt
     4201 aataaaacag tagtagaaaa gattcataaa atgaacaaaa aaatcttcgt ttggactgtt
     4261 aatagtgaca gtttaattga gaaaatgctt gacctaaatg tagatagtat tattacagat
     4321 aatccatacc ttatagagag tgcaatttat tggaagaaaa atggatttat tagtcaagtg
     4381 tcagattatt tattttaaat attctataat tgtattatcc agtaaaagag atattattaa
     4441 aatcaataat atctcttttt aacataaata aggttgttaa attaaggtta ttcttataaa
     4501 aattgtgctt atcagctata aacaaggaat aaaagtgaaa aaattgctct gagattatta
     4561 cagttaaaag agttttaaga tgacagagag atagattgtc aactgtatat ttaataaatc
     4621 ttgttattta ttaaatttta tataagtaaa atatataaat taataaatct tatgctagaa
     4681 tatatatgaa aaacaatata aatttggagg attaaaaaca tgacagagcg agaaaaaatg
     4741 ttggcaggag aactttacga ttgtggtgat attgagttgt taactcaatg gcataaagca
     4801 aaaggcctag taagagatta taaccaaaca gattcctcgg atttggaaaa aaaagaaaaa
     4861 atattaaatg agttacttgg tggtaagggt aaaaacttat ggattactgc accgtattat
     4921 gtagattatg gaaataatat ttattttgga aataattgtg aagtcaatat gaattgtacg
     4981 tttttagatg ataataaaat tatcattggg gataatgcac taatcgcacc aaacgtacaa
     5041 atatatactg catttcatcc aactaatgct ggtgatagat ttggtaatgc caaagaagat
     5101 ggttcatttg aattttgtaa aactcaaaca gctccagtta ctattggaga taatgtttgg
     5161 attggcgggg gtgctattat catgccaggt gttactattg gagataatgt agttataggt
     5221 gcaggaagta ttgttacaaa agatattcca agtaatatga ttgcatacgg aaatccatgc
     5281 cgtattattc gagaaaataa ataaagttaa attttcttgc aaaatctcaa aaaacacctt
     5341 tcttacttta tacatagggt attttctaat actttcacat ccatattcat ttgttaaatt
     5401 tttgtggaat gtagttttta tcacttctat cttacatttc caccattttt ttataaaagt
     5461 cattccttta tagatttatt tataatagta tatattctat ttgatgtttg aataagcact
     5521 tatttttaag taaaatacta acttttaggt tagtattttg ctatagtatg atgattaagt
     5581 aagaataaaa tactataata cataaagctt aataaatata atttttattt tacttagata
     5641 ttttcttttg attagcaatc aaaagtagtt ataatttttt taattaataa gagtaac
//
LOCUS       DAYAPA010000178         5471 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.183, whole genome shotgun sequence.
ACCESSION   DAYAPA010000178 DAYAPA010000000
VERSION     DAYAPA010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5471
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.183"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            95..1276
                     /locus_tag="ACGI59_003567"
     CDS             95..1276
                     /locus_tag="ACGI59_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFG9741319.1"
                     /translation="MNKKYIKAGNLIDGTGNSIMQNKGIIIDGDTIVEIEDIRDDLNG
                     YDVYDYLDKTVMPGIMNCHVHLTMEPVGNPKIYYDNVSDVELVVNTIKQLDAYLDSGV
                     TYIRSLGCPKYVDVQLKNLIDKGSIDGPGIVTSGPVICMTGGHGYYFGIESDGVDECR
                     KSARTVLKNGVDCVKIMATGGVTTDGVEPGSPQLTLEEMKAAVDEAIKAGKITATHAQ
                     GRTGIKNAVLAGITSIEHGVYLDDEIIDLMLERGTYLVPTVAAPYFILNAGKESGITE
                     ATLRKCEIVAIPHKESFLKAYKKGIKIAVGSDAGTSYNEHGKTYYEMKLMSDYGMDNM
                     DIIVAATKTASELLRIDKNYGTLEKGKKADVIVVNGNPVENIDVLADVLAVFKLGKKV
                     R"
     gene            complement(1418..2335)
                     /locus_tag="ACGI59_003568"
     CDS             complement(1418..2335)
                     /locus_tag="ACGI59_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotA family spore coat protein"
                     /protein_id="HFG9741320.1"
                     /translation="MENNKCREDFRFTQEYEEDYPNTNERYYENYQVADRYYNYPNKY
                     KEPKIKQCCCKKSMREALELLRYDALRPFVNFNQFAFISDFFIVGANLVGIDLSAPPK
                     DNLSGLDGTFERFSACNCDLIDIAGRVSYPIPVPLTLEGLINTIGTIPGVAELIALID
                     AVIPPTIDLGAILDAILAAIIDFILAASTPLANVDLASLCNLKAVAFDITPADYEDFI
                     ASLGYYLDKKHYKECNCNCDCDDCCCNKGILDNLYMSNINNQVTVVAGSLVLTGVEVL
                     GKKNDVIVLGNSNDSRIYFVCVDSIDYIA"
     gene            2588..3382
                     /locus_tag="ACGI59_003569"
     CDS             2588..3382
                     /locus_tag="ACGI59_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9741321.1"
                     /translation="MIEVDKLCKSFTRVVKDDKNKSSIRKLKKVKTKKEEFFAVNNVS
                     FKVMEGEIVGILGPNGAGKTTLLRMLGGILTPTSGSINISGYDYSIDRNSAKKEIGYL
                     SGNTKLYGRLSPRELLTTFASLYEMSKEDIEESIENIVKIMDMSEFIDNRIENLSTGQ
                     TQRTSIARCLIHSPKVYIFDEPTLGLDVLSSASIIDFMKNEKLRGKTVLYSTHYMEEA
                     ETLCDKIFMIHNGKIIASGTPQSLKEESGVNNLRDVFIKLARKEDI"
     gene            3383..5443
                     /locus_tag="ACGI59_003570"
     CDS             3383..5443
                     /locus_tag="ACGI59_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit/CPBP
                     intramembrane protease"
                     /protein_id="HFG9741322.1"
                     /translation="MRSKIVKYIFKKEMLDILRDKKTLFMMIVVPLLLYPLLMLLMIQ
                     VMNMSASSMNSKDINIAFNNKPNKVLVSMIEDDNLEKDTKNTSNDKGKIRVLDVDNYK
                     KSLEENKIDAYIKMEEKKSSINYQIYINSSQENSSNALKRIETVLDKYKRFTIEKTLS
                     EKGLDVQATLEPITYSTVDVAKTEEVAGYFLGQILPFILIIGVLLGSIYPAIDVMAGE
                     KERGTLETLFTLPISNLELVMGKYMAVSLSAIVTAILNILSMGLTMAFILVSGGISSQ
                     FGFGNFNYGDLVLPIITTLICICLFSMVVSAISMCVCSLAKSFKDAQNYITPLMLIIM
                     IPSYVSMIPNIVLDRVTAVIPVVNISLLIKSVLSFKSDLNLIALVLASNVAFVILAII
                     LLSKMFNSEEILFGNSKSFSFLEKRSNIKKGSIPTVSDGVILYAVGLVLLIYVGSLVQ
                     IKFGMAGIIMTQFMIISLPLLFAYYIKADFKEVFSLTVPKLKHVFGSIVLWIGGYILI
                     MIITQLILFLFPQNMEVAESLNKALFIKDSVLLNLLAIALLPAICEEMFFRGFIFSSF
                     SKSKDKNKSIKLAIICSGVLFGIMHMDFIRIIPTSILGIIFAYSVYKSGSIFVSMLLH
                     FLNNSVAVVLNHYTTGNITNLYKFVEVDFSNLNVLKFALILLISLILIMLGLRVLDRK
                     SLRN"
BASE COUNT         2092 a          623 c          908 g         1848 t
ORIGIN      
        1 gaaatagaat gcttatagtg gtaaaattaa ttattagaat attagaaata ataaataaat
       61 aatacattgt acttaaaagg gggagaaatt taagatgaac aaaaaatata ttaaagcagg
      121 gaatttaatt gatggaaccg gaaatagtat tatgcaaaat aagggtataa ttattgatgg
      181 agatactata gttgaaattg aagatataag agatgattta aatggatatg atgtgtacga
      241 ttacttagat aaaactgtta tgccaggcat tatgaattgt catgttcact tgacaatgga
      301 gccagttgga aatccaaaga tatattatga taatgtatca gatgtagagc ttgtagtaaa
      361 tacgataaaa caacttgatg cttatttgga ttcaggagta acatatatta gaagcttagg
      421 ttgtcccaaa tatgtggatg tacaacttaa aaatcttata gacaaagggt ctatagatgg
      481 tcctggtata gttacttcag gtccagttat atgtatgaca ggagggcatg gatattattt
      541 tggtattgaa agtgatggag tagatgaatg tagaaaatca gccagaacag ttttaaaaaa
      601 tggagtagat tgtgtaaaga taatggcaac tggaggtgta acaactgatg gagttgagcc
      661 agggtcacca caattaactt tggaggaaat gaaggctgct gtagatgaag ctataaaagc
      721 aggtaaaata actgccacac atgcacaagg aagaactggt ataaaaaatg cagtgcttgc
      781 aggaattact tctatagaac atggtgttta tttagatgat gaaataattg atttgatgct
      841 tgaaagagga acttacttag ttccaactgt ggctgcacca tattttattc taaatgcagg
      901 aaaagaaagt ggaataactg aagctactct tagaaagtgt gagattgttg ccattccaca
      961 caaagagtca ttcctaaaag catataaaaa aggaattaaa attgcagttg gttctgatgc
     1021 aggaacttct tataatgaac atggaaaaac atattatgaa atgaaactaa tgtcagatta
     1081 tggtatggat aatatggata taatagttgc tgctacaaaa acagcatctg aacttttaag
     1141 aattgataaa aattatggaa ctctagaaaa aggaaagaaa gcagatgtta tagttgtaaa
     1201 tggaaatcca gtagagaata tagacgtact tgcagatgtg ttagcagtat ttaaacttgg
     1261 taaaaaagtt agataaatag tataaaaata aaaaagagtg tctaaagaaa tttttaaata
     1321 attatagaca ctctttttta ttagtaataa ttaaatcttt tatactttag aaaatagatt
     1381 ctattttata tttagatata actctttaaa atcattctta tgcaatataa tctatagaat
     1441 ctacacatac aaagtatatt cttgaatcat tagaatttcc aagtactata acatcatttt
     1501 tcttacctag aacttcaaca ccagttagaa ccaaactacc agctactaca gtaacttgat
     1561 tatttatatt tgacatataa agattatcta ggataccttt attacaacag caatcatcac
     1621 aatcgcagtt gcaattacat tctttgtaat gttttttatc aagatagtaa cctaaagatg
     1681 ctatgaaatc ttcataatct gcaggtgtaa tatcaaatgc aacagcttta agattacaca
     1741 atgatgctaa atctacgttt gctaatggag tagatgcagc aagtataaaa tcaattatag
     1801 cagcaagtat tgcatctaat atagccccaa ggtctatcgt aggaggaata actgcatcaa
     1861 taagtgcaat taattcagct actcctggta tagttcctat agtattaatt aatccctcaa
     1921 gagttaaagg gactggaata ggataagata ctctaccagc tatatctatt aaatcacagt
     1981 tacaagcaga aaatctttca aaagtaccat caagtccaga taaattatct tttggaggag
     2041 ctgaaagatc tatacctacc aaattagcac ctactataaa gaaatctgag ataaaagcaa
     2101 attgattaaa gtttacaaaa ggtcttagag catcatatct tagaagttct aatgcttctc
     2161 tcatactttt tttacaacaa cattgtttta ttttaggttc tttatattta tttggataat
     2221 tatagtatct atcagctact tgataatttt catagtatct ttcatttgta tttggataat
     2281 cttcctcata ttcttgtgta aatctaaagt cctctctaca tttattattt tccactaaat
     2341 acactccttt tatattttaa tttaaatgta tagataatta agcttatcta tatagtaata
     2401 ttatatttat aatgtaaaaa aatgttacaa aagatattat aaacgaataa aaaataataa
     2461 tttgtcagat aaacttgtaa atttttatga ataatccatt ctaaaacaaa aaactgtgta
     2521 taaaaaaggt ttatatgtta tatttatata aatataacat atgaacgaga aaggagtggt
     2581 ttgataaatg attgaggtag ataaattgtg taagtctttt acaagggttg taaaagatga
     2641 taagaataaa agttcaataa gaaagctaaa gaaagtaaaa actaaaaaag aagaattttt
     2701 tgctgtaaat aatgtttcat ttaaagttat ggaaggtgaa attgttggaa tattaggtcc
     2761 aaatggtgca ggaaagacta cattacttag aatgttaggt ggcatattaa caccaacatc
     2821 aggaagtata aatatttctg gatatgatta ttcaatagat agaaattcag caaaaaaaga
     2881 aataggttat ctctctggaa atacaaaact atatggaagg ctttctccga gagaacttct
     2941 aacaactttt gcaagtctat atgagatgtc aaaagaagat atagaagaat caattgaaaa
     3001 tattgtaaaa ataatggata tgagtgaatt tatagataat agaattgaaa atctatcaac
     3061 tggtcagact caaagaacat ctatagctcg ttgtttaata cattctccaa aagtatatat
     3121 ttttgatgag ccaacactag gtctggatgt tttaagtagt gcatctatta tagattttat
     3181 gaagaatgaa aaactaaggg gaaaaactgt tctttactca actcattata tggaagaagc
     3241 agaaacttta tgtgacaaga tttttatgat tcacaatggg aaaataattg catcaggaac
     3301 accacagagt ttaaaagagg aatctggagt aaataatttg agagatgtat tcataaaact
     3361 tgccagaaag gaggacattt aaatgagaag taagattgta aaatacatat ttaaaaaaga
     3421 aatgttagac atattgagag ataaaaagac tttatttatg atgattgttg tgccacttct
     3481 tttatatcct ttacttatgt tattaatgat tcaagtaatg aatatgtctg caagtagtat
     3541 gaatagtaag gacattaata tagcttttaa taataaacct aataaggtgt tagtatccat
     3601 gattgaagat gataatttag aaaaagatac taaaaatact tcaaatgata aaggcaaaat
     3661 aagagtgtta gatgtagata actataaaaa gtcattagaa gaaaataaaa ttgatgctta
     3721 tataaagatg gaagaaaaaa aatcatctat aaattatcaa atatatataa attcttctca
     3781 agaaaattct tctaatgcac taaaaagaat tgaaactgtt ttggataaat ataaaagatt
     3841 taccatagaa aaaactttat ctgaaaaagg attagatgta caagctactt tagaaccaat
     3901 aacatatagc actgtagatg ttgcaaagac ggaagaagtg gcaggatact ttttaggtca
     3961 gatacttcca tttatattaa taataggagt tttactaggt tcaatatatc cagcaataga
     4021 tgttatggct ggagaaaagg aaagaggtac attagaaaca ttatttacac tgccaatatc
     4081 aaacttagaa ctggttatgg gaaaatatat ggcagtttct cttagtgcaa tagtaacagc
     4141 aattttaaac attttatcta tgggactgac tatggcattt attttagtta gtggaggtat
     4201 tagtagccaa tttggctttg gaaactttaa ttatggagac ttagtcttac caattataac
     4261 tactttaatt tgcatatgtc ttttttcaat ggtagtttca gcaataagta tgtgtgtatg
     4321 ttcattagca aaaagtttta aagatgctca aaactacata acacctctta tgttaattat
     4381 tatgatacca tcatatgttt ctatgatacc aaatatagta ttagatagag tgacagctgt
     4441 aatacctgta gtaaatatat ctttacttat aaaaagtgta ttatctttta aaagcgattt
     4501 aaatttaata gccttagttt tagcatcaaa tgtagccttt gttatattgg caatcatact
     4561 actgtctaag atgttcaatt ctgaggaaat actttttggt aatagtaaga gtttttcttt
     4621 cttagagaaa agaagtaata taaaaaaagg gtctatacca actgtaagtg atggtgttat
     4681 tttatatgca gtaggattag ttctattgat ttatgttggt tctttagttc aaattaaatt
     4741 tggtatggca ggtattataa tgacacaatt catgataatt tctttaccat tattatttgc
     4801 atactatatt aaagctgatt ttaaagaggt atttagcttg acagttccta aattaaagca
     4861 tgtgtttggg agcattgttc tttggattgg tggatacata cttattatga ttataacaca
     4921 gctaatttta ttcttatttc ctcaaaatat ggaagttgct gaaagtttaa ataaggctct
     4981 ttttatcaaa gatagtgttt tactgaattt actagcaata gctttattac cagctatatg
     5041 tgaagaaatg ttttttaggg gatttatatt ttcttcattt agtaaatcaa aagataaaaa
     5101 taagtctatc aaactcgcta taatatgtag tggggtacta tttggtatta tgcatatgga
     5161 ttttattaga ataattccaa catcaatatt aggtattata tttgcataca gtgtgtataa
     5221 atctggttca atatttgtat caatgttatt gcatttttta aataatagtg tagcagttgt
     5281 gttaaatcat tatacaactg gaaatataac caacttatat aaatttgttg aagttgattt
     5341 ttcgaactta aatgtgctta aatttgcctt aattcttcta ataagtctta tattaattat
     5401 gttaggatta agagttttag atagaaagtc tttaagaaat taagtataaa gttattatat
     5461 agattaaaat a
//
LOCUS       DAYAPA010000179         5435 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.184, whole genome shotgun sequence.
ACCESSION   DAYAPA010000179 DAYAPA010000000
VERSION     DAYAPA010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5435
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.184"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(448..648)
                     /locus_tag="ACGI59_003571"
     CDS             complement(448..648)
                     /locus_tag="ACGI59_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011723062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFG9741323.1"
                     /translation="MNNGIVKWFNNEKGFGFISMESGDDVFAHFSAIQTSGFKSLEEG
                     QKVSFDIVKGARGPQAENITIL"
     gene            complement(873..1049)
                     /locus_tag="ACGI59_003572"
     CDS             complement(873..1049)
                     /locus_tag="ACGI59_003572"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9741324.1"
                     /translation="MFRDKMDKCTHMLTAYISNSYDYCDFIDTQLDDFILEYGEKVVE
                     SCLHQVMVLVSKYN"
     regulatory      complement(1103..1192)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(1372..1572)
                     /locus_tag="ACGI59_003573"
     CDS             complement(1372..1572)
                     /locus_tag="ACGI59_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFG9741325.1"
                     /translation="MPKFTCNLKKYRQLKELTQEQLAEKVGVRRETIMRLEAGKYNPS
                     LKLAIDISKIVDTPIDELFIFD"
     gene            complement(1574..1924)
                     /locus_tag="ACGI59_003574"
     CDS             complement(1574..1924)
                     /locus_tag="ACGI59_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3796 domain-containing protein"
                     /protein_id="HFG9741326.1"
                     /translation="MIQKKFFMGFCGFLGFLSLRYFSSGNVADLTYIGFFAFFSNFII
                     AKINGDKADERYVQDEKAAMAFTGQLAIIELFILWCITIVSRNIELMCVLLSITYAIT
                     LNVYAIKLYILEEK"
     gene            2086..2274
                     /locus_tag="ACGI59_003575"
     CDS             2086..2274
                     /locus_tag="ACGI59_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9741327.1"
                     /translation="MTKYDFNFKLKVAKFYLNREGRDILIVKQYGVYDHSQVERWVNI
                     LGESELKIKKFSILYGSS"
     gene            complement(2751..2951)
                     /locus_tag="ACGI59_003576"
                     /pseudo
     CDS             complement(2751..2951)
                     /locus_tag="ACGI59_003576"
                     /inference="COORDINATES: protein motif:HMM:TIGR04223.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3310..3798)
                     /locus_tag="ACGI59_003577"
     CDS             complement(3310..3798)
                     /locus_tag="ACGI59_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HFG9741328.1"
                     /translation="MKNKKGFTLVELLVVIAIIGILAIVALPALFKNIEKAKIAKLEA
                     DISAIKSASLSYYADESKYTEGNIIWWTKKDGKITVNSGIGDEDPLAHKIENLGMPYN
                     GSYTLVSSNGSEEYLELNIIIDGEISKSGLDKLEEDYGSSIKIPNDKNMIITFLSNKS
                     DN"
     gene            4602..4976
                     /locus_tag="ACGI59_003578"
     CDS             4602..4976
                     /locus_tag="ACGI59_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HFG9741329.1"
                     /translation="MKEEILIEKLLRAELIVMEYLWKKDTLMPKKEIVKEIKQIYGWH
                     KSTIKILLKRLVAKRYLARYIINSQSHYKIIDSKEYYTFKKKVLKSSKSIKIMRSLTT
                     THKNVSKEKLDLLDEYYRNLKE"
     gene            5081..5435
                     /locus_tag="ACGI59_003579"
                     /pseudo
     CDS             5081..5435
                     /locus_tag="ACGI59_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430377.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
BASE COUNT         2192 a          646 c          616 g         1981 t
ORIGIN      
        1 ttaaagtatt ttaaatttaa aaacttaaat caaattaact tattgtagat tttttacttt
       61 ttaactaatt ttatatttat tatatttcag tgaaaaaata gaacattgaa acatatagat
      121 atgttttaat tgctttttat taacaaatta ctaaaaatgt tatatcagaa ttgaatacaa
      181 aagaaagagt atgtatttat gagatagttg aaaattttga tgtgccacaa ctaaaattat
      241 tatattacta tatatttaat taaaagagta cttgtaggtt taagtacata ctttataaat
      301 atatttttgt tagatttatt ttataaaaag tatttagact aagtattatt tttaagtatt
      361 ttcttgttga agttattttt tcctttttta caaaattcgt aaataaaaaa aagactctta
      421 aaagagtctt tccgaaaaat ctatatatta taatatagtt atattttctg cttgaggacc
      481 tctagcgcct ttaactatat caaagcttac tttttgacct tcttctaatg atttaaatcc
      541 tgaagtttgt atagctgaga aatgagcaaa tacgtcatct ccactttcca ttgatataaa
      601 tccaaatcct ttttcattat taaaccattt tactattcca ttattcatta aaaatactcc
      661 tattaaccta tatcaagata tacttgattt acttgtgtat taccacaaat attattgtat
      721 catagattaa cataaatgtc tatttaatta taatttttag ttaacatttt tatttattct
      781 taatatgaaa ataaatttaa acattttata ttaccaatat attaatgtaa atgtagataa
      841 aactaaaaag ggttttctag aaaaaaatct gtctaattat acttacttac caataccatc
      901 acttgatgca agcaagattc cacaactttt tctccgtact ctaatataaa atcatctaac
      961 tgtgtatcta taaaatcaca ataatcgtat gaattactaa tataagcagt taacatatgt
     1021 gtacatttat ccattttatc tctaaacata atgtatttat ccccctaaaa aactttcatg
     1081 aaaaacaaaa agaaaatatc tttttggcaa ctggctgaca taacttattt tataagcgtt
     1141 agtccctata gctttgcgtc actaaatttc tctagttttg ccgatttagt tttattctac
     1201 agattaataa tacaatagaa ttagttatca ttcaacatat ctagttgaat aattaataaa
     1261 tttatatatg aactttaaat tatttgaaaa aataaaaagc ccctctgtca ggagtgactt
     1321 aaaaattgaa tttaagtttg tatttaaaac acatttttaa taatgcaaaa actaatcaaa
     1381 aataaacaat tcatcaatag gtgtatctac tatttttgat atatctattg ctaatttcaa
     1441 agaaggatta tactttcctg cttctaaacg cataatggtt tctcttctaa ctcctacttt
     1501 ttctgctaat tgttcttgag ttaattcttt taattgtcta tattttttta ggttacaagt
     1561 gaatttaggc atattatttt tcctctaaaa tataaagttt tatagcataa acatttaaag
     1621 tgattgcata tgtaattgat agtaaaacac acattaattc tatattccta gacactatag
     1681 taatacacca taaaatgaat aattctataa tagcaagctg tcctgtaaat gccatagctg
     1741 ctttttcatc ttgtacatac ctttcatctg ctttatcacc atttattttt gctataataa
     1801 agtttgagaa aaaagcaaaa aatccaatat atgtaaggtc agcaacattg ccagatgaaa
     1861 aatatctaag acttaaaaat ccaaggaaac cacaaaaacc cataaaaaat tttttctgaa
     1921 tcatttttta tcctccttag taattttaaa agagatatat ttatatcttt atgatacaaa
     1981 tatatctctt ttatattctt ttgtcaatag aaaatcagta ttatacaata tttatactgc
     2041 accataattg ttagagcaaa aatctaatgc ttgagatgtt tatgtatgac taaatatgat
     2101 tttaatttca aattaaaagt agctaaattt tatttgaata gagaaggaag agatatttta
     2161 atagtaaaac aatatggagt ttatgaccat tctcaagttg aaagatgggt taatatatta
     2221 ggagagagtg aactaaaaat aaaaaaattt tctatactat atggttcaag ttagatgtta
     2281 taaactatat gatgataact aaatatagtg tacaataaat cgctaagtat tttggaataa
     2341 acaatagcac agttatagca acatgaaaat aaaaattttt acatagtggt gttgatgcct
     2401 tttctaaaat gaaaggaaga ccataatgaa taaaaacaaa aacactaaag aaaaaataaa
     2461 ttaacaagag aacaagaatt agaatatgaa aataaatttt taaaagatga gcttgagttt
     2521 ttaaaaaaga tgtgtgtctc agggaagcca cattaaaaaa cagtggtatc attattaatc
     2581 aaaagaaagt tagatgtatt atcaaaaaat tagaacttaa atgcatagca ttttttcata
     2641 aatcacataa atataactcc tataaaggta taattggaaa agtaaaaaat aataaaacaa
     2701 atagaagatt taaaacatca atagcatatc agaaaataac aaatgatatg ctagaattta
     2761 taaaatatat ctaatgataa aaatggaaaa tcaattataa aaaaggcata tctcgattca
     2821 ttttttgtta tatagtcaaa taagaatact atagatgaaa gatacgattt tgcatatgta
     2881 ttaagtaagt attctgatgt ttttggttga tacacatgca ataaagaagt tgtgtttgca
     2941 gataaattag caaaactata gtataagttg caacaataaa gaaaatgaaa attatcatac
     3001 taaagcataa ggcatgttac aattttatta gataggacat taacatacac taaagtactt
     3061 tttattataa agagtactta tttttttgaa attcattaat gtataaacta tcaagaatat
     3121 tactcaacat aatttaaaaa caacttgaat aggtattttt aaattatgtg atataataaa
     3181 aaatagaagt gtatgccaat acacttctgt agttataact aagcattcta ttcttgagga
     3241 gtgctttttt catttttata accaactctt tttactttaa caataattat cagacctata
     3301 tatttatttt taattgtctg atttattaga taaaaaagtt attatcatat ttttatcatt
     3361 tggtattttt attgaactac cataatcttc ttctaattta tctagaccac ttttacttat
     3421 ttctccatct ataattatgt ttaattctaa gtattcttca ctaccattag atgacactaa
     3481 agtgtacgaa ccattataag gcatgcctaa attttctatt ttatgtgcca aagggtcttc
     3541 atcaccaata ccagagttta ctgttatttt tccatctttt ttagtccacc atattatgtt
     3601 tccttcagta tattttgatt catctgcata gtagctaaga gacgcacttt ttattgcact
     3661 tatatcagct tcaagtttag ctatctttgc tttttctata tttttaaata atgctggaag
     3721 tgctactatt gccaatattc ctattatagc aattactact aataattcca ctagagtaaa
     3781 tcctttttta tttttcattt atttcccctc catataaaat taataatata taaattttta
     3841 ttttgttaat ttctactttc tatattttac catatataga caaaaaaatc actttctatg
     3901 acaaatatac tattatttta caatttatgg aaatgattta aaatgacatt tcttgttaga
     3961 aaagatataa gaaatggatt aaaataaaga aaactacagt catatcaatg tcaataatat
     4021 tatttaaaag cagatgaaaa taaaaagagg actcagaaaa taatgtctat ttaattatac
     4081 ttacttacca ataccataac ttgatataaa caagattata ctacattttt ttatgctcta
     4141 gtataaaatc atctaactgt gtatctataa aatcaaaata attatatgaa ctatcagtat
     4201 aataagtcaa aatatgtata catgaataca tattttaaaa gatatatatt attacatacc
     4261 taaaaaactt tcataaaaac aaaaataaaa tatgctttta gtaacttgtt aacataactt
     4321 ataatttatt ttataagcat tagcctatat agctttgtgc tactaaatct ctttgattct
     4381 aacgatttag tgctattcta caactaaaat aatacaatag agctatttat tgtttggtag
     4441 cattgtttgt aaattaaaca aatttataca tatgaatatt attttacatt caaaaaaaca
     4501 gactaaatat gtagaatatt tggtaaaact tacctctagt ttttgtctta aaaagctcct
     4561 attattaact taagttagaa gaattaagga agtgacataa attgaaagaa gaaatattaa
     4621 tagaaaaatt attaagagca gaattaatag taatggagta tttatggaaa aaagacactc
     4681 taatgcctaa aaaagaaatt gtaaaagaaa taaaacagat ttatggatgg cataaaagta
     4741 caataaaaat actactaaaa aggctagttg ctaaaaggta tttggctaga tatattataa
     4801 attctcaatc tcattataaa ataatagata gtaaagaata ttatactttt aagaaaaagg
     4861 tactaaaatc tagtaaaagc ataaaaataa tgcgttcact taccactaca cataaaaatg
     4921 taagtaaaga gaagttagac ctattagatg agtattatag aaacttaaaa gaatgatata
     4981 gtaaaaatcg aatgtaatta tttaccttaa ttcttttgtt tgtaaaactt tattgataca
     5041 gaatttaaca tatacataaa ttggtaaagg aatgtatact atgaaaatta gtaaattacc
     5101 agaagcggaa ttaaaagtta tgagatatat ttgggaatca gaaaaggtat taatatcaag
     5161 agaaatcacc aagtctatgg aacaaaaata tgaatggaaa gatacaacta tatttacagt
     5221 attaaaaaga ttactaaaga agagagtttt tagaagcgga gaaaatagat aagtgtacac
     5281 actataatgt attagtaaaa aaaaataata tcagagattt taaacaaaag aatttttaaa
     5341 gaatacacat aaaaattcaa tattaagttt aatgtcagca ctatgtagta aggatgatga
     5401 agtagatggt gatattttaa atttattaga agaat
//
LOCUS       DAYAPA010000180         5405 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.185, whole genome shotgun sequence.
ACCESSION   DAYAPA010000180 DAYAPA010000000
VERSION     DAYAPA010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5405
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.185"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            <1..315
                     /locus_tag="ACGI59_003580"
     CDS             <1..315
                     /locus_tag="ACGI59_003580"
                     /inference="COORDINATES: protein motif:HMM:NF016432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HFG9741330.1"
                     /translation="SLRGIELNGMPKGNNVSSPIENELIYVERLENEKIYLQIKKESI
                     NNMLNLLDDFEKNLIELRYFKKLQYKQISYELNISELYVGKKRKKALDKILPFAKKYN
                     LI"
     gene            504..926
                     /locus_tag="ACGI59_003581"
     CDS             504..926
                     /locus_tag="ACGI59_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009530967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFG9741331.1"
                     /translation="MAKLTEKQKRFCDYYIETGNATEAAIRAGYSEKTAKVIGAENLT
                     KPYLKSYIDERIGQLESNRIADAKEVMEYLTKIVRNEAKEEVVVVSEYGPEIIKKDVS
                     IRDRNKAAELLGKRYRLFTDKVEVEGTGIVQIVDDIDE"
     gene            919..2184
                     /locus_tag="ACGI59_003582"
     CDS             919..2184
                     /locus_tag="ACGI59_003582"
                     /inference="COORDINATES: protein motif:HMM:TIGR01547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HFG9741332.1"
                     /translation="MNEIRLKNIIAPSFYNVHKLIKQDVYTHYWFKGGRGSTKSSFIS
                     LEIVLGMMRDAQQGKLTNAVVIRRVKDTLRGSVYEQIQWAIYTLGVQNEWEIPESKLQ
                     MTYKPTGQVILFKGADKPKKLKSTKVSKGYIKYVWYEEVDEFEGMDKIRNVNQSLLRG
                     GNTYNVFYSFNPPESQRNWTNMEVIEKREDKFIHHSNYLSVPKEWLGEQFIVEAEHLK
                     KVNPTKYEHDYMGVVTGTGGEVFTNVTVREISNEEINRFDRVRRGLDFGYAKDPLSYI
                     VMNYDKTRKRLYIFFELYKVALGNSKAVELILKENTSNKKVIADSAEPRSINEFKKLG
                     LKIIGARKGPDSVEHGIKFLSEEVEEIIIDPIRCPNAKREFLGYELEKDKEGNFKDEY
                     PDKNNHTIDAVRYGMEDVMLKRKGLSILK"
     gene            2200..3585
                     /locus_tag="ACGI59_003583"
     CDS             2200..3585
                     /locus_tag="ACGI59_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0046798 - viral portal complex [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0051027 - DNA transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFG9741333.1"
                     /translation="MNIEVVKKLIKKHVPYHSDVISKSIISERYYKNKNDILFKTSNR
                     EEEETENPLRNADNRISSSFYSLLVNQKASYLFTYPPTFDVSNNRTNKAITEVLGDIY
                     PKICKDLCINASNSGVSWLHLWKDKNSKLNYGVVDSKQVIPLWSSDLNKKLIGVLRVY
                     DEIDENGDNYIIYEYWTDTECQAYRKLDDLDIDELEVYNMFSITYGNGDILDNSNTLK
                     HDFGELPFIPFFNNNIKTSDLDTVKSLIDTYDKVFSGFVNDLEDIQEIIFILTNYGGT
                     DLKSFLEDMKKYKTVEVESNGMDDKSGLSTLTIDIPVEAREKLLMMTRKAIFEQGQGV
                     DPQPIDFGNASGVALKYLYSLLELKAGLMETEFKLSFGKLIRMICKVLNLECNIVNQT
                     WTRNAIANDLELAEICAKSVGIVSDKTVCKNSPIVEDVEYEIAQIKKEKEEAQREYDD
                     LIPNDGVIDET"
     gene            3575..5062
                     /locus_tag="ACGI59_003584"
     CDS             3575..5062
                     /locus_tag="ACGI59_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HFG9741334.1"
                     /translation="MKHKDYWRKRFEQLEEAQNNKSIKYYLELEKQYKLAMSSIEKDI
                     LAWYNRFAKNEGISLLEAKKLLNTRELEEFKWSVEEYIKYGKENAINQKWMKELENAS
                     ARVHITRLEALKLQIQQQVEVLYGNELDGIDKLMRDIYTSGYYHTAFNVQQGVNVGWS
                     LMSLDTNRINKVISKPWATDGLNFSERIWGKHRPALINELYTKLTQSIIRGENPKNLV
                     NDFAKRFNVSKSQAKNLIMTESAFFASASRKDCFNDLDVEKYEIIATLDLRTSNICRE
                     LDGKVFDMKDYQVGVTAPPFHCRCRTTTAPFFSDEEGYRAARGEDGKTYYVPSSMKYK
                     EWYEKYINKNIKLSDNEQLAINKYVSSDSYKINEKLRRGLSLTSEDKNFISNLDSALD
                     KMPNYKGNVNRSLYFFNEKEKIDFLNKHQIGKEIVYNEYISTSSRGEYNPSGQVELNI
                     ISTNGKDIRKYNPQEAEILFKRNSKFITTDNFEYDGKHYITMKEV"
     gene            5064..5222
                     /locus_tag="ACGI59_003585"
     CDS             5064..5222
                     /locus_tag="ACGI59_003585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9741335.1"
                     /translation="MKKDNEKLFSAPRWTEIPQAKVVGERTLTEEEVKEAQEIRKKII
                     KRKINNKG"
BASE COUNT         2262 a          525 c         1030 g         1588 t
ORIGIN      
        1 agtttaagag gaatagaatt aaatggaatg cctaaaggaa ataatgtttc ttcaccaata
       61 gaaaatgagt taatatatgt tgaaagattg gagaatgaga aaatatatct acaaattaaa
      121 aaagaatcga ttaataatat gttaaattta ttagatgatt ttgagaaaaa tctaatagaa
      181 ctaaggtatt ttaaaaaatt acaatataaa caaataagtt atgagttaaa tataagcgaa
      241 ttatatgtag gaaaaaagag aaaaaaagca ctagataaaa tacttccctt tgctaaaaaa
      301 tataatctaa tatgaaagtc ttgtttgtgt ccgatttatg accgattaat gattgattta
      361 gtatagaaac atgtcacatc aatatactat gagataagat ataatggtag tatgaaacaa
      421 gtgtataata tatccccttg aaaaaggcta agtttacccc aaacttagcc ttttttatat
      481 ttaaaacaaa gtgaggtggt gatgtggcta aattaactga aaaacaaaag aggttttgtg
      541 attactacat tgaaactggc aatgcaacag aagcagcaat aagagcagga tatagtgaaa
      601 agactgctaa agtaataggt gctgagaact taacaaaacc atacctaaaa agttacattg
      661 atgaaagaat aggtcaactt gagtcaaata ggatagcaga tgcaaaagaa gttatggagt
      721 atttaactaa gatagtaagg aatgaagcaa aagaggaagt ggttgtagtt tctgaatatg
      781 gtccagaaat aataaaaaaa gacgtaagta taagagatag aaacaaagct gctgaattat
      841 taggtaagag gtataggttg ttcactgata aggtagaagt tgaagggact ggtatagttc
      901 aaatagtaga tgatatagat gaatgagatt agattaaaaa atattatagc tccaagcttc
      961 tataatgtac ataaacttat taagcaagat gtttatactc attattggtt taaaggtggg
     1021 agaggtagta caaaatcatc ttttatatca ttagaaattg ttttaggtat gatgagagat
     1081 gctcaacaag gcaaattaac aaatgcagtt gtaattagaa gagttaaaga cacacttaga
     1141 ggttctgttt atgaacagat acagtgggct atttacacat taggggttca aaatgaatgg
     1201 gaaataccag aatctaagct tcaaatgact tataaaccaa caggtcaagt aattttattt
     1261 aaaggagctg ataaacctaa gaaattaaaa tctactaaag tgtctaaagg ttatataaaa
     1321 tatgtttggt atgaagaagt agatgaattt gaaggtatgg ataaaattag gaatgttaat
     1381 caatctttgc ttagaggtgg gaatacatat aatgtttttt actcatttaa tccaccagaa
     1441 agtcaaagaa attggactaa tatggaggtg atagaaaaga gagaagataa atttattcat
     1501 catagtaatt atttatctgt accaaaagaa tggttaggag aacaatttat agtggaggct
     1561 gaacatctta aaaaagtaaa tcctactaag tatgagcatg attatatggg tgttgtaact
     1621 ggtacaggtg gagaggtatt tactaatgta acggtaagag aaatatcaaa tgaagagata
     1681 aatagatttg atagagtaag aagaggtctt gactttggat atgctaaaga ccctttatct
     1741 tatattgtta tgaactatga taaaacaaga aaaaggttat atatattctt tgagctttat
     1801 aaagtagcat taggaaattc aaaagctgtt gagcttattt taaaagaaaa cacaagcaat
     1861 aaaaaagtaa ttgcagatag tgcagaacct agaagtataa atgagtttaa aaagctaggt
     1921 ttaaaaataa ttggtgctag aaaaggtcca gatagtgtag aacatggaat caaattctta
     1981 agtgaggaag tagaagaaat aataatagac cctataagat gtccaaatgc taagagagaa
     2041 tttttaggat atgaacttga aaaagataaa gaaggtaatt ttaaagatga gtatccagat
     2101 aaaaataatc atactataga tgctgttaga tatggtatgg aagatgtaat gttaaaacga
     2161 aagggattat caatacttaa ataaaagtga ggtgaataga tgaacataga agtagttaag
     2221 aagctgatta aaaaacatgt tccctatcat tctgatgtaa tatctaaatc aattatatct
     2281 gaaagatact ataagaataa aaatgatata ttatttaaaa cttctaatag ggaagaggaa
     2341 gaaactgaaa atccactcag aaatgctgac aatagaatat caagtagttt ctatagcttg
     2401 ttagttaatc aaaaggcatc atacttattt acttatccac caacttttga tgtatctaat
     2461 aatagaacaa ataaagctat tacagaagtt ttaggtgata tctatcccaa aatatgcaaa
     2521 gacttatgta taaatgcaag taatagcgga gtatcatggt tacatctatg gaaagataaa
     2581 aacagtaagt taaattatgg tgtggttgac agtaaacaag taataccttt atggtcctct
     2641 gatttaaata aaaaacttat aggggtactt agagtatatg atgaaataga tgaaaatggt
     2701 gacaactata taatctatga atattggaca gatacagagt gccaagcata tagaaaacta
     2761 gatgacttag atatagatga actagaagtt tataatatgt tctctataac atatggaaat
     2821 ggagatatat tagacaatag caatacactt aaacatgatt ttggagaatt accattcatt
     2881 ccattcttta ataataatat aaagacaagt gatttggata ctgtaaaatc gttaattgat
     2941 acttacgaca aagtttttag tggttttgta aatgatttag aagatataca agagattata
     3001 tttattttaa ccaattatgg aggaacagat ttaaaaagct tcttggaaga tatgaagaag
     3061 tataaaactg ttgaggtaga aagtaatggg atggatgata aaagcggttt aagcacactt
     3121 acaattgata ttccagttga agctagagaa aagttactta tgatgacaag aaaggctata
     3181 tttgagcaag gacaaggtgt tgacccacaa ccaattgact ttggtaatgc aagtggtgta
     3241 gcactcaaat atttatactc acttttggaa cttaaagcag gtcttatgga gactgagttt
     3301 aaattgagtt ttggaaaact aataagaatg atatgcaagg ttttgaattt ggaatgtaat
     3361 atagtaaatc aaacatggac aagaaatgct attgccaatg atttagaatt agcagagata
     3421 tgtgcaaaaa gcgttggtat tgtttctgat aaaacagtat gtaaaaatag tcctattgta
     3481 gaagatgtag agtatgaaat agcacagatt aagaaggaaa aagaagaagc tcaaagagag
     3541 tatgatgatt tgataccgaa tgatggtgtt atagatgaaa cataaagatt attggaggaa
     3601 gagatttgaa caattagaag aagctcaaaa taacaaaagt ataaaatatt atcttgaatt
     3661 agaaaaacaa tataaattag ctatgtctag tatagaaaaa gatatattag catggtataa
     3721 cagatttgcc aaaaatgaag gaatatcttt attagaagct aagaaactgc tgaatacaag
     3781 agaactagaa gagtttaagt ggagtgtcga agaatatatt aaatatggta aagaaaatgc
     3841 tataaatcaa aagtggatga aagagttaga aaatgctagt gcaagagttc atataacaag
     3901 gcttgaagct ttaaagttac aaatacagca acaagtagaa gttctctatg gaaatgaact
     3961 tgatggtatt gataaactaa tgagagatat ttatacaagt ggatattatc atacagcttt
     4021 taatgttcaa caaggagtaa acgttggttg gagtttaatg agtcttgata ctaacagaat
     4081 aaataaagtt atctctaaac catgggcaac agatggatta aactttagtg aaagaatttg
     4141 gggtaaacat agacctgctt taatcaatga gctatatact aaactgacac aatcaattat
     4201 taggggtgag aatccaaaga acctagtaaa tgactttgct aagagattta atgtgtctaa
     4261 atctcaagct aagaatttaa taatgactga atcagctttc tttgcatcag caagtagaaa
     4321 agattgtttc aatgatttag atgtagagaa atatgagatt attgctacat tagatttaag
     4381 aacttcaaat atatgtagag agctagatgg aaaagtattt gatatgaaag attatcaagt
     4441 tggagttaca gctccaccat ttcattgtcg ttgtaggaca acaacagctc ctttttttag
     4501 tgatgaagaa ggctatagag cagcaagagg agaagatgga aaaacatatt atgtaccatc
     4561 tagtatgaag tataaagaat ggtatgagaa atatattaat aaaaacatta agttatcaga
     4621 taatgaacaa cttgcaatta ataaatatgt aagtagtgat tcttataaaa ttaatgagaa
     4681 acttagaaga gggttatcat taacaagtga agataaaaat tttataagta acttagatag
     4741 tgcattagat aagatgccta attataaggg aaatgtgaat aggtccttgt atttctttaa
     4801 tgaaaaagaa aaaatagact ttttaaataa acatcaaata ggtaaggaaa tagtatataa
     4861 tgaatatata tctacatcca gtagagggga atacaatcca agcggtcaag ttgaattaaa
     4921 tattataagc acaaatggca aggatataag aaaatataat cctcaagaag cagagatttt
     4981 atttaaaagg aatagtaaat ttataactac tgataatttt gaatatgatg gcaaacatta
     5041 tataacaatg aaagaggtgt aatatgaaaa aggataatga aaaattattt tcagcaccaa
     5101 gatggacaga aataccacaa gctaaagtgg taggggaaag aacattaaca gaagaagaag
     5161 tgaaagaagc gcaagaaatt agaaaaaaaa taattaaaag aaagattaat aataaaggat
     5221 agaagcactt gctaaatgat aaattagtag gtgcttttat tatgtaaaaa tttattgaga
     5281 gggtgatttg aaatgattaa attatatatt ttatcaataa ttgtattttg tacagggctt
     5341 tatttattta aaatgaagat tgatagtaat gaggaattga ttaagattct taaaaataaa
     5401 aaagt
//
LOCUS       DAYAPA010000181         5327 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.186, whole genome shotgun sequence.
ACCESSION   DAYAPA010000181 DAYAPA010000000
VERSION     DAYAPA010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5327
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.186"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            103..1008
                     /locus_tag="ACGI59_003586"
     CDS             103..1008
                     /locus_tag="ACGI59_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HFG9741336.1"
                     /translation="MFFKDIELNSKKELDPYFDLVDYEACEYCFSTLYMWQHVYKTGY
                     YIGEDFAVLVGEYEGDSFSILPLAKKDKLPEVVDFVLEYFSKNNKKIYLRGITTEVVE
                     FLKEKYPGRFEYIEERDLFDYIYDAESLRTLAGKKNQKKRNHINYFLKEYAGRYEAKL
                     LDKENFDECLVLMKEWESNKEENNEFDESMDDELIGIKKIFNHYDILKDKVKVFGVYV
                     DGKLEAFSIGELLNPNMALIHIEKANPDIRGLYPFINQQFLVSEFKDVEFVNREEDLG
                     IEGLRKAKLSYHPCRFVEKYSVREA"
     gene            1017..2201
                     /locus_tag="ACGI59_003587"
     CDS             1017..2201
                     /locus_tag="ACGI59_003587"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFG9741337.1"
                     /translation="MEIRYAKEEEIESIKEIWSYCFNDTESFMKYYFNDKYKSENTVV
                     ALDEGKIISSLQLNQYKLLLNSKVYNTSYVVGVSTLPEGRGAGYMNKVMKFTLNELYK
                     KGQLVSILMPIDYRLYRRFGYEHCYDQIEYTINTDDLKNFKSSGKMIKSNLSQIDDLI
                     QIDRTFLNEVNGNVLKDEHYYENLFKEIQSEDGFLYIHEGNEKDGYIVYFLQEDKMFV
                     RELFYKNIDALKSMLKFIYNHNTQCKIVTISTPTIDKIRFILDNPKDSDIKIKPFMMG
                     RVINVKKFIEDIDIENDINSSFNLLIEDKFIDENNGLFKISIQNKKVSVEQLDKKGAE
                     KPQEDFDIKLDINTLTQLSFSYIDVNEAIFLNDIKDVSEETLETLNCIFSKKNNYINE
                     YI"
     gene            2316..3617
                     /locus_tag="ACGI59_003588"
     CDS             2316..3617
                     /locus_tag="ACGI59_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HFG9741338.1"
                     /translation="MGVIIIQIRRPLLIILMFVVAISFIITNNTKNDEELNDKIITIQ
                     GVVKGKIEKKRYNQYKVGVFLINDYTKNKNLKIGQKVNITGKFKSLDKMKYDDFDYGR
                     YIKSTGYKGIVYINNYKIIDKNKLYSLIGEIKFYISKTYRYLYKKNSDFINSILLAEV
                     ENLTEEQKEIFTRTGTSHVISISGLHTGILCVIISFLLRGINKLYKLLILSIFITLYC
                     IMVGASPSIIRSIAFVMIFYLSIFIDRKKDGISALSLIGIILIMNNPYVIYNVSFQLS
                     FLATLSILIFYNKINSIIKLSMVSLTISSNILTLPIIYYTFKGIPLLSIIGNLIIVPF
                     VGVIMYLSIASLIVFKVSVVIAKIISFFNSTFIESIFFLLEKISNLSFAYINIENPKF
                     YIVVIYYIGVFFYIFYIEGKEIKEQENELQGYYKECKREKL"
     gene            3571..4614
                     /gene="holA"
                     /locus_tag="ACGI59_003589"
     CDS             3571..4614
                     /gene="holA"
                     /locus_tag="ACGI59_003589"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFG9741339.1"
                     /translation="MNYKDIIRNVKEKNFEKMYLFYGREYYLIENAIKAFKDSLNEGM
                     LDFNLDIIDGKEIVLNQLISSIETLPFMDDRKIVIIKDFELLKGKKKNFTDSDEKYLI
                     EHLDNIPDTTTIVFVVYGDVDKRKSLVKKIGNNGIVFDCDKLSDMDLFKWIKKSFALN
                     DVIIDNSQIMYFIEQEGYRDKSSEKTLSDLENEINKISSFVGKGNNVTNDVIDKLSQK
                     KVENDIFKLIDYIGEQNASNAMKILNDMIQEGESVLGIFSMIARQFKIIMQVRQLQLD
                     GYSTKLIADKLKMHQFVVGKALKQTKNFSDDIIVEILNYILESDYKIKTGLIRDTLAV
                     EMLVSRYCKREAI"
     gene            complement(4801..5067)
                     /gene="rpsT"
                     /locus_tag="ACGI59_003590"
     CDS             complement(4801..5067)
                     /gene="rpsT"
                     /locus_tag="ACGI59_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFG9741340.1"
                     /translation="MANIKSAKKRISVIEKKTALNRVRKSQIKTAIRRFEDAVAAGNR
                     EDAVAKFQYAQKRIYQVASKGTIHKNAAARKVAKLAQKLNGMNA"
BASE COUNT         2146 a          469 c          829 g         1883 t
ORIGIN      
        1 taaacaatat aaatgaaatt aataccataa taaaattgga gttttttata tatacaaaat
       61 aaaattttta aaattaagca ataaaaaagg taggaggatt ttatgttttt caaagatatt
      121 gaactaaatt caaaaaaaga gttagaccca tattttgatt tagtagatta tgaagcttgt
      181 gagtactgtt ttagtacttt atatatgtgg caacatgttt ataagacagg atattatata
      241 ggtgaagatt ttgccgtttt agttggggaa tacgaaggag atagcttctc aatattacca
      301 cttgctaaaa aagataaact tccagaagtt gtagattttg tccttgaata tttttctaaa
      361 aataataaaa aaatatactt aagaggtatt actactgaag tagtagagtt tttaaaagaa
      421 aaatatccag gtagatttga gtatatagaa gaaagagatt tatttgacta tatttatgat
      481 gcagaaagtt taagaactct agctggtaaa aagaatcaaa agaagagaaa ccatataaac
      541 tatttcttaa aagagtatgc aggtagatat gaagctaaat tattagataa agaaaacttt
      601 gatgagtgtt tagtactaat gaaggaatgg gaaagtaata aggaagaaaa taatgaattt
      661 gatgaaagta tggatgatga attaataggt atcaaaaaga tatttaacca ttatgacata
      721 ttaaaagata aggtaaaagt gtttggtgtt tatgtagatg gaaaattaga agcttttagt
      781 ataggagagc ttttaaatcc aaatatggca ttgattcata tagaaaaagc taatcctgat
      841 ataagaggtc tgtatccatt tataaatcaa caatttttgg taagtgaatt taaggacgtt
      901 gagtttgtaa atagagaaga agatttaggt attgaaggat taagaaaagc taaactttct
      961 tatcatcctt gcagatttgt tgaaaagtac agtgttaggg aggcttaata gactatatgg
     1021 agattaggta tgctaaagaa gaagagatag aaagtataaa agagatttgg agttattgtt
     1081 ttaacgatac ggagagtttt atgaagtact attttaatga taaatataaa tctgaaaata
     1141 ctgtagtagc tttagatgaa ggaaaaatta tttcttctct tcagctaaat cagtataagt
     1201 tattactaaa tagcaaggta tataatacat catatgtagt aggagtttct acacttccag
     1261 agggaagagg agctggatat atgaacaaag taatgaaatt tactttaaac gaattgtata
     1321 aaaaagggca attagtttct atattgatgc ctattgacta tagactttat agaagatttg
     1381 gatatgagca ttgttacgac cagattgaat atactattaa cacagatgat ttaaaaaatt
     1441 ttaagtcaag tggaaaaatg ataaagtcta atttatcaca aattgatgat ttgatacaaa
     1501 tagatagaac ttttttaaat gaggttaatg gtaatgtgtt aaaagatgaa cattattatg
     1561 aaaacttatt taaagagatt caaagtgaag atggattttt atacattcat gaaggaaatg
     1621 aaaaagatgg atatatagtt tattttcttc aggaagataa aatgtttgtc agagagttat
     1681 tttataaaaa tatagatgcc cttaaatcaa tgcttaaatt tatatataat cacaatactc
     1741 aatgtaagat agttactata tctactccta caatagataa gataagattt atattagaca
     1801 accctaaaga tagtgatata aaaataaaac cttttatgat gggaagagtt ataaatgtaa
     1861 agaagtttat tgaagatata gatattgaga atgatattaa tagctcattt aatttattaa
     1921 tagaagacaa gtttatagat gaaaataatg gtctatttaa aatttctata cagaataaga
     1981 aggtatctgt agagcagtta gataaaaaag gtgcagaaaa gcctcaagaa gattttgata
     2041 ttaaattaga tataaatacc ttaacacagt tatcattttc ttatatagat gtaaatgaag
     2101 ctattttctt aaatgatata aaagatgttt cagaagaaac actagaaaca cttaattgta
     2161 ttttttctaa aaaaaataac tatataaatg aatatatata ggtagagtac gttaaaagat
     2221 tttaaaaatc ttttgacgta tttttttgtt tagataacaa tttatcttgt ttaaaaattt
     2281 tctatttgaa aataatctaa ataatatact tttatatggg agtgattata attcaaataa
     2341 gaagaccatt attaattata ttaatgtttg tggtagctat atcatttatc attacaaata
     2401 acacaaaaaa cgacgaagaa cttaatgata agataataac tatacaagga gttgtcaaag
     2461 gaaaaattga aaagaaaaga tacaatcagt acaaggtagg ggtttttcta ataaatgatt
     2521 atacaaaaaa taaaaatttg aagattggtc agaaagttaa tattacaggt aaattcaaaa
     2581 gtttagataa aatgaagtat gatgattttg attatggaag gtatataaaa agtactggat
     2641 ataaaggtat agtgtatata aataactata aaattattga taaaaataaa ctttactctt
     2701 taatagggga aataaagttt tatattagta aaacttatag gtacttatat aagaaaaact
     2761 cagattttat aaattctata ctattagcag aagttgaaaa tttgactgag gaacaaaaag
     2821 aaatttttac tagaactgga actagtcatg taatatctat atcaggtctt catacaggaa
     2881 tattatgtgt aattatatcc tttctattaa gaggtataaa taaattgtat aaattattaa
     2941 tactgtcgat ttttataact ttatattgta taatggtagg tgcgtctcca tccataatta
     3001 ggtctatagc atttgttatg attttttatt tatcaatatt tattgataga aagaaagatg
     3061 gaatatcggc attgtctcta attgggataa ttttaattat gaataacccc tatgttatat
     3121 ataatgtaag ttttcaattg tcttttttag ctacactttc tatattaata ttctataata
     3181 aaataaatag tattataaaa ttaagtatgg tatcattgac aatatcatca aatatattaa
     3241 ctttacctat aatttattat acatttaaag gtataccatt gctttcaatt ataggaaatt
     3301 taattatagt accatttgtg ggagtaataa tgtatttaag tattgcaagt ttaatcgtat
     3361 ttaaagtaag tgtagtaata gctaaaatta tatctttttt taatagtaca tttatagaaa
     3421 gcatattttt tttactagaa aaaatcagca atttaagttt tgcatacatt aatatagaaa
     3481 atcctaaatt ttatattgtt gttatttatt acataggagt gtttttttac atattttaca
     3541 tagaaggaaa agagataaag gagcaagaga atgaattaca aggatattat aaggaatgta
     3601 aaagagaaaa actttgaaaa aatgtatctt ttttatggaa gagagtatta tttaattgag
     3661 aatgcaataa aagcatttaa agatagtcta aacgagggaa tgcttgattt taacttagat
     3721 ataatagatg ggaaggaaat agtcttaaat cagcttataa gctctataga aacacttcca
     3781 tttatggatg atagaaagat agtaataata aaagactttg aactattaaa aggaaagaaa
     3841 aagaatttta cagatagtga tgagaaatac ttaatagagc atttagacaa tattccagac
     3901 accacaacta tagtttttgt tgtatatgga gatgtagaca aaagaaaatc attagttaag
     3961 aaaataggaa acaatggaat tgtttttgat tgtgataagc tttctgatat ggatttgttt
     4021 aaatggataa aaaaatcttt tgctttaaat gatgttataa tagataattc tcaaatcatg
     4081 tattttattg aacaagaagg atacagagat aagagtagtg aaaagacatt atctgattta
     4141 gaaaatgaaa taaataaaat aagttcattt gttggtaaag ggaataatgt aaccaatgat
     4201 gtaattgata aattatctca aaaaaaagtt gagaatgata tattcaaatt aatcgattat
     4261 attggagaac aaaatgcttc taatgcaatg aaaatattaa atgatatgat acaagaaggc
     4321 gaatctgtat taggtatatt ttctatgata gcaagacaat ttaaaataat tatgcaagta
     4381 agacagttac aacttgatgg atattcaaca aaacttatag ctgataaatt aaaaatgcat
     4441 caatttgttg taggaaaagc attaaagcaa actaaaaatt tttctgatga tataattgtg
     4501 gaaatattaa actatatatt agaaagtgat tataaaataa agacaggtct tataagagat
     4561 actttagcag ttgaaatgtt ggttagtaga tactgtaaaa gagaagcaat ttaaaaaatc
     4621 tatatattaa taaaaaataa atagcaagat atgttatagt tcagagtaga tgtaagctaa
     4681 attaagtttg atatacaatc aactataata tgtctaacta tttatttttt attgtataaa
     4741 tttacaaaat aaaaaacccc tacatagggg ttattatata taagaaatat aaactggtaa
     4801 ttaagcgttc ataccgttta atttttgagc taatttagct acttttctag ctgcagcatt
     4861 cttatgtata gttcctttag aagctacttg gtatattctt ttttgagcgt attggaactt
     4921 agcaacagca tcctctctgt ttccagctgc aacagcatct tcgaatcttc ttattgcagt
     4981 ttttatttga gattttctaa ctctatttaa agcagttttc ttttcgataa cacttattct
     5041 tttcttagct gattttatat ttgccaccgt gttcacctcc ctgtattttt ttatcatatt
     5101 catcgtcaac atattagatt ttaacagaaa taaatctaaa aatcaaggat tattatttat
     5161 atattttaat aatgtttttt attttgattt tttagactgt aaattgtgta taaataattt
     5221 gcaatatctt gtttttaaat tggtatattt tgtgttttaa atattttgac atatataaat
     5281 taatatttta tttttgtaac aaatattttg agataataca aactatt
//
LOCUS       DAYAPA010000182         5320 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.187, whole genome shotgun sequence.
ACCESSION   DAYAPA010000182 DAYAPA010000000
VERSION     DAYAPA010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5320
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.187"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            139..924
                     /locus_tag="ACGI59_003591"
     CDS             139..924
                     /locus_tag="ACGI59_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFG9741341.1"
                     /translation="MSLNSIDRKKEILKLLDSKGKVNTKELVKRLEVSSETIRRYLEE
                     LENENKLKKVYGGAIKINYGVIEPEHIKRNNTNLDKKQSIAKYAAKFVNDNESIIIDE
                     GTTNLQIVDYIISVNNLKIITNSYPVLSKLISYENQGLFDGEVIFIGGKVNLKHQRTA
                     CPMSIDTMKNLYVDKAFISSEGILDSFGISSIDQNKALLSKEYIKNSKESFVLCDSSK
                     IGIASMYKIADLEELDFVICDVEPPNEWKDKLNEINITWIEAK"
     gene            1203..3113
                     /gene="phnW"
                     /locus_tag="ACGI59_003592"
     CDS             1203..3113
                     /gene="phnW"
                     /locus_tag="ACGI59_003592"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HFG9741342.1"
                     /translation="MKKIYGEKIKAVVFDWAGTTVDYGCFAPLNVFIEIFKRRGIDVT
                     MEEARKPMGKLKIDHIREMCEMDRIKNIWSDKFGKVPTEDDVNELYAEFEPMLFETLE
                     EYTTPIPHVVETIEKLRKNGLKIGSTTGYTREMMNIVEPNAAKKGYSPDFLVTPSEVS
                     QGRPYPWMCYKNAEALGVSPMSSMVKVGDTISDVKEGVNAGMWSVAVIKGSSELGLTQ
                     EEVENMDKEELKAKMSIVSKKFKEAGAHFVIETMAELEDILIKIENETIKSDFVPEND
                     YILLTPGPLSTTKSVRASMLKDWCTWDVEYNNLVQDVRRRLVSLATQNTDKYTSVLMQ
                     GSGTFSVEAIIGSTISKDGKLLVIANGAYGKRMKDICNYLNIQFVDCTFKDIEAVDLN
                     VVENLLKENKDITHISMVHCETTTGRLNPIQEVGKLAKEYNKIYIVDAMSSFGGIEID
                     VEDFNIDFLVSSSNKCIQGVPGFGFIIANKEKLSKCKGIAKSLSLDVYAQWETMEKNN
                     GKWRFTSPTHVVRAFYQALLELEEEGSVEKRYARYKENQFTIASRLKSLGFDTLVNDN
                     AQSPVITTFLYPKNAKFEFMEFYTYLKDNGFVIYPGKLTDIDTFRIGSIGEVYPTDME
                     RLADVIEKFINR"
     gene            3472..4029
                     /locus_tag="ACGI59_003593"
     CDS             3472..4029
                     /locus_tag="ACGI59_003593"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9741343.1"
                     /translation="MNEELSSLRFDGVGKCKGGIFDNVNISGTGKIEGSVKCSKFDSS
                     GSAKVFGTTECNEFFTSGLSKVEGNILASKVEISGLLRCTGNINSVEIDTSGIINVEG
                     SIKSDIIHGEGYLKANENIDCENINLSGILECNGFLNCEEVNISLNGTSNFNEVGASS
                     INIKKGHGDYCKKFRMLEKKHISSR"
     gene            4122..4334
                     /locus_tag="ACGI59_003594"
     CDS             4122..4334
                     /locus_tag="ACGI59_003594"
                     /inference="COORDINATES: protein motif:HMM:NF024570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4004 family protein"
                     /protein_id="HFG9741344.1"
                     /translation="MEELISKKDLLSKTNISYGQLYRWKRKNIIPEEWFIKKSAFTGQ
                     ETFFPRDKILERIELILSMKEETSLD"
     gene            4900..5307
                     /gene="tnpA"
                     /locus_tag="ACGI59_003595"
     CDS             4900..5307
                     /gene="tnpA"
                     /locus_tag="ACGI59_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_045009167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HFG9741345.1"
                     /translation="MEVTHGRGYVYLVQYHIVWCVKYRHPVLTNEIEEDLINILNKIA
                     EDNKFQILECNSDKDHIHLLINCSPQHYIPDIIKALKGVSARLLMKKHGEQLKKKLWG
                     GHLWNPSYFVATVSENTEEQIRKYINNQKKQVK"
BASE COUNT         2198 a          524 c          992 g         1606 t
ORIGIN      
        1 tttttaatga taatacaaag tcttttgaaa ataactttaa ttaagtaaaa aatttaatac
       61 ataaatatga tttcataaat cttaggtatc attgtaatat tataggagaa aaatatatta
      121 tatttaggag gaaaatgaat gtctttaaat tcgatagata gaaaaaaaga aatacttaaa
      181 ttattagatt caaaagggaa ggtaaacact aaagaattag taaaaagatt agaagtatca
      241 tcagaaacaa taagaagata tttggaagaa ttagaaaatg aaaataaatt aaaaaaggta
      301 tatggtggtg ctataaaaat aaattatgga gtgattgaac cagaacatat aaagagaaac
      361 aatactaacc tagataaaaa gcaaagtatt gctaaatatg cagctaagtt tgtaaatgac
      421 aatgagtcaa ttatcataga tgaaggtact accaatttgc agattgtaga ttatataata
      481 tctgtaaaca atttaaaaat tattacaaac tcttatccag ttctatcaaa attgatttct
      541 tatgagaatc aaggtttgtt tgatggagaa gttatattta ttggtggaaa agtaaattta
      601 aaacaccaaa ggacagcatg tccaatgtca atagatacta tgaaaaactt atacgtagat
      661 aaagctttta tatctagtga aggtatactt gatagttttg gaattagtag tatagaccaa
      721 aataaggcat tgttgtcaaa agaatatata aaaaattcaa aggaaagttt tgtattatgt
      781 gatagttcta aaataggaat tgctagtatg tataagatag cagatttaga ggaattagat
      841 tttgttattt gtgatgtaga acctccgaat gaatggaagg ataagttaaa tgagataaat
      901 ataacatgga tagaagcaaa ataaaccctt agttttatat aatacaacta tgctcaatac
      961 aactaaattc ataaaatata gtcagttcat agattaagat tagaaagtaa attttagatt
     1021 gtaacttagg attttatggg aatatgatac aaaataaaaa caactaaata caactaaaaa
     1081 cagaaattta atatatattt aacctaaata tgatgtataa ttaacatatg aaagttatac
     1141 tttaaaagta aaaacaatta aaaacacaaa aattctaaaa agaatctatg gaggctataa
     1201 atatgaaaaa gatatatgga gaaaaaataa aagcagtagt attcgattgg gctggaacaa
     1261 cagtagatta tggatgtttt gcacctctta atgtatttat agagatattt aaaagaagag
     1321 gtatagatgt aacaatggaa gaagcaagaa aacctatggg aaaattaaaa atagaccata
     1381 taagagaaat gtgtgaaatg gatagaataa agaatatttg gtcagataag tttggtaaag
     1441 tgccaactga agatgatgta aatgaacttt atgctgaatt tgagccaatg ttatttgaga
     1501 cattagaaga gtatacgact ccaattcctc atgttgttga aactattgaa aagttgagaa
     1561 aaaatggatt aaaaataggt tctacaacgg gttatacaag agagatgatg aacatagttg
     1621 aaccaaatgc agcaaagaaa ggatactcac ccgattttct tgtaacacca tcagaagtat
     1681 ctcaaggaag accgtatcct tggatgtgct ataaaaatgc tgaagcttta ggggtgtctc
     1741 ctatgagttc tatggtaaaa gtaggagata caataagtga tgtaaaagaa ggcgtaaatg
     1801 caggaatgtg gagtgttgct gtaataaaag gttcaagtga gttagggctt acacaagaag
     1861 aagtagagaa catggataag gaagaattaa aagctaagat gagtatagtt tcaaagaaat
     1921 ttaaagaggc aggtgcacat tttgtaatag aaactatggc tgagttagaa gatatactaa
     1981 taaaaataga gaatgaaact attaaatcag attttgtacc agaaaatgat tacatattac
     2041 ttacaccagg accactatct actactaaat cagtaagagc atctatgtta aaagattggt
     2101 gtacttggga tgttgaatat aataacctag tacaagatgt tagaagaaga ttagtatctc
     2161 tagcaaccca aaatacagat aaatacacaa gtgtattaat gcaaggaagt gggacttttt
     2221 cagtagaagc tataatagga agtactattt caaaagatgg taaacttctt gtaattgcaa
     2281 atggagcata tggaaagaga atgaaagata tatgtaatta tcttaacata caatttgttg
     2341 actgcacatt taaagatata gaagcagtgg atttaaatgt agtagaaaat ttattaaaag
     2401 aaaacaagga tataacacat atatcaatgg ttcactgtga aactactaca ggtagattaa
     2461 atcctataca agaggtaggg aaacttgcaa aagaatataa caaaatttat atagttgatg
     2521 ctatgagtag ttttggggga attgagattg atgttgaaga ttttaatata gattttttag
     2581 ttagtagttc taacaaatgt atacaaggag tacctggatt tggttttata atagctaata
     2641 aagagaaatt atctaaatgt aaaggtatag ctaaatcatt atctcttgat gtgtatgcgc
     2701 aatgggagac tatggagaaa aataatggta aatggagatt tacttcacct acacatgttg
     2761 taagagcttt ttatcaagct ttattagaat tggaagaaga aggttctgtt gaaaaaagat
     2821 atgctagata taaagaaaat cagtttacaa tagcttcaag attaaaatct ttaggctttg
     2881 atactttggt gaatgataat gctcaatcac ctgtaataac aactttctta tatcctaaaa
     2941 atgcaaagtt tgaatttatg gaattttata cttatttaaa agataatgga tttgttatct
     3001 atccaggtaa attgactgat atagatacat ttagaatagg tagcataggt gaagtctatc
     3061 caactgatat ggagagacta gctgatgtaa tagaaaaatt tatcaataga tagaagaaga
     3121 tagtgtcttg taggttacag aatagtgcgc aaagcctact cctttaggtg tgtgagatga
     3181 tagcacaaac agtgtataaa aagtattgac agtagaattt aaagacttat attttagaag
     3241 tttatacaat gaatatataa acattgagta atagggatat attttattta aaccacaatc
     3301 taagaaatta gatttatagt ttaaataaag catttcccta ttatttttat gcctatttat
     3361 agaaaataaa tgatagttgc tttattaagt attattatca tacttgacaa cgtaacaatg
     3421 ttttgttata ttaggtttaa agataaaata cggtaaagaa aggatatcat tatgaatgaa
     3481 gaattaagtt cactaagatt tgatggtgtt ggaaaatgta aaggtggaat ttttgataat
     3541 gttaatatat caggaacagg caaaatagaa ggaagtgtaa aatgtagtaa atttgattct
     3601 tctggttcag caaaagtttt tgggacaaca gaatgtaatg aattttttac atctggttta
     3661 agtaaagtag aaggaaacat actggcatca aaagttgaga tatcgggact tcttagatgt
     3721 acaggaaata ttaattctgt tgaaattgat actagtggaa ttattaatgt agaaggaagt
     3781 ataaaatcgg atattattca tggagaagga tatttaaaag caaatgagaa tatagattgt
     3841 gaaaatatca atttaagtgg aatcttggag tgtaatggat ttttgaattg tgaagaagtt
     3901 aatattagtt taaatggaac ttctaatttt aatgaagttg gagcaagtag tataaatata
     3961 aaaaaggggc atggagatta ttgcaaaaaa tttcgtatgc tagaaaaaaa gcatatctct
     4021 agtagataat tatgatgttg gaaatattga atacagtgat aatttatcag tttcagataa
     4081 ttctactgta aaaaacagta taaaggtggg ctagaagagc tatggaagaa ttaatatcta
     4141 agaaagattt attgagcaaa actaatatat cttatggaca attatataga tggaaaagaa
     4201 aaaacataat accagaggaa tggtttataa aaaaatcagc ttttacaggt caagagactt
     4261 tctttccaag agataaaata ctagaaagaa tagagttaat attgtctatg aaagaagaga
     4321 cttctcttga ttaaaaaata gctatgcaca cttgtgactt tagtcatgag ttagtagcta
     4381 aaatagtcag catataggga aacttatatg tagagatagg atagaaccta tccaatacta
     4441 cttgaattgc tggaaacccc taaagctaac caaactaaaa cataaggatg aaataaacct
     4501 aagtgtgaaa gttacgaaag tagaaaaaat tggttagatg gtataaggtt aaatcctaag
     4561 tactttaaaa tgggcaatca gcaggtaagc tccgaatagg aaaaacttca acgactattc
     4621 ctcttgaggg aagtacacta caagctatcg gtagtggaag tgggtagacc ttaacggata
     4681 atgccgaagg ataagatata gtctgtgctt gcatgaaagt gtaagaagtt cataagagaa
     4741 ctgcataggt agtagcgaac ttatgtgaac gacaacctca aaaatgataa aagttaagat
     4801 ttgcgtttct tacgtatgtt atttaaaaat aaatataaaa accttaagaa tacttgtttt
     4861 aagtaattac tatgttaata ttatgttggg tgatgaaaaa tggaagtgac tcatggtaga
     4921 gggtatgtgt atttagtaca ataccatata gtatggtgtg taaaatatag acatccagta
     4981 ttaacaaatg aaattgaaga agatttaatt aatatattaa acaaaatagc tgaagataat
     5041 aaatttcaaa tattagaatg taatagtgat aaagatcaca tacatttact aataaattgt
     5101 tcaccacaac actatatacc agatataata aaagcattaa aaggtgtatc agcaaggctg
     5161 ttaatgaaaa aacatggaga acagttgaaa aagaaattat ggggaggaca tctttggaat
     5221 cctagttatt tcgttgctac tgtatcagaa aatacagaag aacagataag aaaatatatt
     5281 aataatcaga aaaaacaagt aaagtgaggt ggcagtagtg
//
LOCUS       DAYAPA010000183         5303 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.188, whole genome shotgun sequence.
ACCESSION   DAYAPA010000183 DAYAPA010000000
VERSION     DAYAPA010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5303
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.188"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            125..1930
                     /locus_tag="ACGI59_003596"
     CDS             125..1930
                     /locus_tag="ACGI59_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFG9741346.1"
                     /translation="MLSKLKEFQQEMIKYTETVASVLDVDIEIVDDRLIRISGTGLYK
                     SKINESVVTEGFIYDNVIQTGQELVVLDICDNQLCIECSHYMKCLNKVIIAVPIKYNN
                     RTIGVIGAISTDKTKKVEISAKIDNYLKFVNHICDLISMKIEEHEASKNSSRKMDMMI
                     EIIENVEKGVIILDINSKISYINNIALKKLDIDKNIIENIVNIVSVESSSNGHELLEI
                     DIDNKIYNINAKIIPVYPYINQYDKIIIFDKTYINHKGHGKVNSGWGNSDIESIIGNS
                     EAMLKVKERTKKLAKSNSTVLITGESGTGKELIARAIHAEGSRWNKPFIAINCAAIPE
                     NLLESELFGYIKGAFSGASSGGKVGKFELANEGVIFLDEIGDLSMPLQAKLLRVLQER
                     KFARIGSNKLIDLDIRVIAATNKNLLKLVNEGKFRDDLYYRLNVIPINLPPLRERKDD
                     IEAIMMKFASKYSLELGIQLNKIEENVMNMLINYNWPGNIRELENAVEYMMNLVGDDG
                     IIYKDMLPLDILNYYNINGNIYKNKDVNIIFEDDIVGGIVENQERILSIKELELTYIN
                     KLLNKYGRDTKTKKKIAKDLGIGLATLYRKLEEEQ"
     gene            2007..2294
                     /locus_tag="ACGI59_003597"
     CDS             2007..2294
                     /locus_tag="ACGI59_003597"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9741347.1"
                     /translation="MQYAIELYYDKKTEKQLFDLSKKIADEKISTKYLEWKTRPHLTL
                     ACFNDVDEACCIDKLKGFAQNHKVIPAYIGSVGMFNDTKTVFVSPIMNSSM"
     gene            3169..4383
                     /gene="dpaL"
                     /locus_tag="ACGI59_003598"
     CDS             3169..4383
                     /gene="dpaL"
                     /locus_tag="ACGI59_003598"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HFG9741348.1"
                     /translation="MREIKWKLNNLPKSEEKEKGIEFLKDEEIAKAKAFHESFPQYEK
                     TPLVKLDNLAKSLGVSGIYVKDESYRFGLNAFKVLGGSYSMGRYLAQRLDTDISELGY
                     DKLTSKEIKEKLGEITFFTATDGNHGRGVAWTANKLGQKSVVLMPKGSSEFRLNKIKG
                     EGADASITDLNYDDAVRLANDYAEADDHGVMVQDTAWDGYEEIPAWIMQGYGTMAKEA
                     IEQLKEYGVDRPTHVFVQAGVGSLAGAVQGYVASIYDECPITVVVEADEADCYYKSAE
                     AGDGKPRFVGGDMPTIMAGLACGEPNTIGFEVLKNYSSAFVSAPDWVSAKGMRILGNP
                     LRGDEKVISGESGAVTTGALVSILESEDLKDLREALKLDENSKVLLISTEGDTDPDKY
                     RDIVWNGECQSK"
     gene            4713..>5303
                     /locus_tag="ACGI59_003599"
     CDS             4713..>5303
                     /locus_tag="ACGI59_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HFG9741349.1"
                     /translation="MLNETRKKQLTEVCQDLIRNASYSGQEENVVKAIEENFALLGFD
                     SWSRDRYGNIVGCIKGNKPGKKILFDGHIDTVPVPDASKWSVPPFEARIVDGKIYGRG
                     TSDMKGQVSAMMAAVSYFAEDTNKDFEGELYVAGVVHEEIFEGVSAREISKAVQPDYV
                     VIGESSELNLKIGQRGRGEIVVETFGKPAHSANPEKG"
BASE COUNT         2178 a          540 c         1036 g         1549 t
ORIGIN      
        1 atattgtctg ttttaacgca aatatataaa taagtaatgt tttatatata ttttaaagag
       61 tgactatatg ttaaaatata gctaaactga taatttggta aattaaattc taatggggga
      121 aactatgtta agtaaattaa aagaatttca acaagaaatg ataaaataca ctgaaacagt
      181 agctagtgtg ttagatgtag atattgaaat agttgacgat agattaataa gaattagtgg
      241 tacaggtctg tataaatcaa agataaatga aagcgttgtt actgagggct ttatttatga
      301 caatgttata cagacaggtc aggaattagt cgtattggat atatgtgata atcagctttg
      361 tatagaatgt tcccattaca tgaaatgcct aaataaagta ataatagcag ttccaataaa
      421 gtataataat cgaactatag gagtaattgg tgctattagt acagataaaa ctaaaaaagt
      481 agaaataagt gcaaaaatag ataattattt aaaatttgtc aatcatatat gcgatttaat
      541 ttctatgaaa atagaagaac atgaagcgtc aaaaaatagc agtagaaaaa tggatatgat
      601 gatagaaatc attgaaaatg ttgaaaaagg cgttataata ctagacataa atagcaaaat
      661 atcttatata aacaatatag ctctaaaaaa attagatata gataaaaata taatagaaaa
      721 tatagtaaat atagtatctg ttgaatcttc ctcaaatgga catgaattac tagaaattga
      781 tattgataac aaaatttata atataaatgc caaaataatt ccggtatatc catatataaa
      841 tcaatacgac aaaataataa tatttgacaa gacatatatc aatcataaag gacatggaaa
      901 agtgaatagt ggctggggaa atagtgatat agagtctata attggaaatt cagaagcaat
      961 gttaaaagta aaagagagaa ctaagaaatt agcaaagtca aattcaactg tacttataac
     1021 tggggaaagt ggtacgggta aagagctaat tgctagggct atacatgctg aaggtagcag
     1081 atggaacaag ccatttattg cgataaactg tgctgctatt ccagaaaatc tccttgaaag
     1141 tgagctattt ggctatataa aaggtgcatt tagtggagct agtagtggag gtaaagtagg
     1201 taaatttgaa cttgcaaatg aaggtgttat atttcttgat gaaataggag atttgtcaat
     1261 gcctttacaa gcaaagttac ttagagtttt acaagagaga aaatttgcga gaattgggtc
     1321 aaataaactt atagatttag atataagagt gattgctgct acaaataaaa acttattgaa
     1381 attagtaaat gaaggtaagt ttagggatga tttatactat agattgaatg taattcctat
     1441 aaatttgcca cctcttaggg aaagaaagga tgatattgag gcaataatga tgaagtttgc
     1501 ttctaaatat tctttagaat taggtattca gttaaataaa atagaagaaa atgtaatgaa
     1561 tatgttgatt aactataatt ggccgggaaa tataagggaa ctggaaaatg cagttgaata
     1621 tatgatgaat ttggttggag atgatggaat aatatataaa gatatgcttc cattagatat
     1681 tttaaactat tataatatta atggaaatat ttataaaaat aaggatgtaa atataatatt
     1741 tgaagatgat atagttggag ggatagtaga gaatcaagaa aggatacttt caattaaaga
     1801 attagagttg acttacataa acaagttgtt gaataagtat ggaagagata caaagacgaa
     1861 gaagaagata gcaaaagact taggcatagg tttggctaca ctatatagaa aattggaaga
     1921 ggaacagtag taatataata ttaaaatgat aggtatttgt gtgtttttat agataggtat
     1981 ttttacttta tggtggaggt gttattatgc aatatgcgat agagctgtat tatgataaaa
     2041 aaacggaaaa gcaacttttt gatttatcta aaaaaatcgc tgatgaaaaa attagtacga
     2101 agtatttaga atggaaaaca agacctcatc ttactttggc ttgtttcaat gatgtggatg
     2161 aggcttgttg tatagacaag ctaaaggggt ttgcacaaaa ccataaggta atacctgctt
     2221 acattggttc agtaggcatg tttaatgata ctaaaacggt gtttgtatca cctataatga
     2281 atagcagtat gtagcagttt cagagagaat tatatgaggt actacaagat tttgattcaa
     2341 atggttggga atggtaccac cccaatcgtt gggtacccca ttgcacaata gcacttactg
     2401 gagatgatga aagtgaggct ttttttcggg caagtgatgt attactgcgt gagttcaaaa
     2461 aaataagtgg tgagtttgtc tcaataggat tggttaaaat tacatttcct gtggaagaaa
     2521 tttttacagt gaatatgaat aggtaacttt aactctgttg tggatgttat gaatattttg
     2581 tatagtgcat ttcatcagat aaatgcgaag ttgcgaattt cgacagctca atagtcgcaa
     2641 gtagggcaca atggtacaca tattgcactt ttgcttattg gggggacgcc catgtaaact
     2701 tctgtaagaa gctatgaaaa gatttaagcc attttggagg ctttgggtaa taaagaatat
     2761 ttgggtaata aagaatataa agtatataaa tttatagagt ttatgcgtaa gaaggttata
     2821 gccatatatt tatagaaaat aaaagtagta atatatctga caaaacaata gacatataaa
     2881 aaataagagc ataataaagg aagaattaag ataaataaac gatttctcga attgataatt
     2941 ctcgtattga taaaaatttc ccaatatgag aattttttct tttataaatc ttatttttca
     3001 cactcaaaat ttataaactt taaagaaatt ttataaaagt atgtaattcc aacataaaaa
     3061 aattgttttt agaataaata atattggcac ggtaattgct ttgctataag gcacaaagat
     3121 aaaaaattaa aatgacaccg aaaaatttga aaggggaata taaaataaat gagagaaata
     3181 aaatggaaat taaataactt acctaaatca gaggaaaaag aaaaaggaat tgaattttta
     3241 aaagatgaag agattgcaaa ggctaaagct tttcatgaaa gctttccaca atatgaaaaa
     3301 actcctttag ttaaattaga taacttagca aaatcactag gcgtaagcgg aatatatgtt
     3361 aaagacgaat cttatagatt tggtttaaac gcatttaaag ttttaggtgg ttcttactct
     3421 atgggtagat acttagctca aagattagat acagatatat ctgagttagg gtatgataaa
     3481 ttaacttcaa aagaaataaa agaaaaatta ggagaaataa ctttctttac agctacagat
     3541 ggaaatcatg gtagaggagt tgcttggaca gctaataaat taggtcaaaa atctgttgta
     3601 ttaatgccaa aaggttcttc tgaatttaga ttaaataaaa tcaaaggaga aggtgcagat
     3661 gctagtataa cagacttaaa ctatgatgat gcagtaagat tagctaatga ttatgcagaa
     3721 gctgatgacc atggagtaat ggttcaagat acagcatggg acggatatga agaaatacca
     3781 gcatggataa tgcaaggata tggaactatg gctaaagaag ctatagaaca attaaaagaa
     3841 tatggagtag atagaccaac acacgtattt gttcaagcag gagttggttc acttgctggt
     3901 gcagttcaag gatatgttgc ttcaatatat gatgaatgcc caataacagt agtagtagag
     3961 gctgatgagg ctgactgtta ctataaatca gcagaagctg gagatggaaa accaagattt
     4021 gttggaggag atatgccaac tataatggca gggcttgctt gtggagaacc aaacactata
     4081 ggttttgaag tgttaaaaaa ttattcatca gcattcgttt cagcaccaga ctgggtatct
     4141 gctaaaggta tgagaatatt aggaaaccct ctaagaggag atgagaaagt aatctctgga
     4201 gaatctggag cagttacaac tggagcatta gtttcaatcc ttgaaagtga agatttaaaa
     4261 gacttaagag aagctttgaa actagatgag aactctaaag tattattaat aagtacagaa
     4321 ggagatactg accctgataa atatagagat atagtttgga atggtgaatg ccaaagtaaa
     4381 taaaagactt aaggaaaaga aaatcttaac aaataaatga aaaatttaag gaaaataaaa
     4441 acttaaataa gtgaaaactt taaataaaat aaaaactaaa acaaatgaaa aatctaaata
     4501 aatgaaaaag taaagcaaat aaaaaagtaa agcaaatgaa aaattcaaaa taaaaaccaa
     4561 ataaaattaa aataaagtct aaataaaaat ttaataaata aaatataaag aaataaaaaa
     4621 tcaatagatt aagtgaaatg ctaataaatt aaaaaaatga aatatcaaaa taaaacgtca
     4681 acaaattaaa aattatatgg gggaatatca aaatgttaaa tgaaacaaga aaaaaacaat
     4741 taactgaagt atgccaagac ttaataagaa atgctagtta ttcaggtcaa gaagaaaacg
     4801 tagtaaaagc tatagaagaa aattttgctt tactaggttt tgatagttgg tcaagagata
     4861 gatatggaaa tatagtagga tgtataaaag gaaataaacc aggtaaaaaa atattatttg
     4921 acgggcatat agatacagtt ccagttccag atgcaagtaa atggagtgtt cctccttttg
     4981 aagcgagaat agtagatggg aaaatatatg gtagaggaac ttctgatatg aaggggcaag
     5041 taagtgctat gatggctgct gtttcatact ttgcagaaga tactaacaaa gactttgaag
     5101 gtgaattgta tgtagcaggt gttgttcatg aagaaatatt tgaaggtgtt tcagctagag
     5161 agataagtaa ggctgttcaa cctgattatg ttgtaatagg tgaatcttca gaattaaacc
     5221 taaagatagg tcaaagaggt agaggagaaa tagtagtaga aacttttggt aaaccagctc
     5281 actctgctaa tccagaaaaa ggt
//
LOCUS       DAYAPA010000184         5283 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.189, whole genome shotgun sequence.
ACCESSION   DAYAPA010000184 DAYAPA010000000
VERSION     DAYAPA010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5283
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.189"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(50..1897)
                     /locus_tag="ACGI59_003600"
     CDS             complement(50..1897)
                     /locus_tag="ACGI59_003600"
                     /EC_number="3.1.21.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DpnII family type II restriction endonuclease"
                     /protein_id="HFG9741350.1"
                     /translation="MKPILKWAGGKTQLLDILRKQMPTGCDFSSMTYIEPFVGAGALF
                     LYLLEEDIFNKYVINDINSKLINLYIKVRDDLEELITELTNLKEQYISYEEGSSDREK
                     MYYDIRDRFNILTNEKNIENEDCIKQASYFIFLNKTCFNGLYRENSRGEFNVPFGRYK
                     NPSFFDKNELEKLSMLLNKRDSRGELKVKILNKNYLNLENIIDKNCFVYLDPPYRPVT
                     IGGFNSYNKGNFNDDSQIELSKFYKVLDKIDTKLMLSNSDPKVLDEEDTFFDDLYSDF
                     NIKRVHAKRFINSKGNGRGFITELLITNYEEVGNQMELVKNIHKNKSKDEIFKELLEN
                     LKGTIASWDYYVNWNKALENTRKIEVQLNILNYLIGKEDIENETKNLLKEYPEVLSVI
                     PIVIASRDNEFKILDPSDEDMFKYKLFNFKKRKSISDEESELATEFLKKIKFLDLLKQ
                     KNIKNLVDYVFGVEVGLDTNARKNRTGSSMETLVERYVKNMCEKNNWSYLTQATVKKI
                     SEKWSIEVPTDKSKRKYDFAINNNGKIVLVEVNFYNGGGSKLKSVANEFTKLDKYIKD
                     GGFTFCWITDGLGWLESSIPLRDAFDKMDYIFNLDMVDKGSLEYIIKNL"
     gene            complement(2325..2674)
                     /gene="ssrA"
                     /locus_tag="ACGI59_003601"
     tmRNA           complement(2325..2674)
                     /gene="ssrA"
                     /locus_tag="ACGI59_003601"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(2832..3287)
                     /gene="smpB"
                     /locus_tag="ACGI59_003602"
     CDS             complement(2832..3287)
                     /gene="smpB"
                     /locus_tag="ACGI59_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFG9741351.1"
                     /translation="MAGKKILATNKRARHEYFIEETYECGIELKGTEVKSIRQGKVNL
                     SDGFASVDNSEVFLKQVHISPYEQGNIFNVDSLRVRKLLLHKHEIRKLIGATTIKGYS
                     LIPLSLYLKNGKVKVEIALAKGKKLYDKRQDMAKKDAQRRIEREMTGKY"
     gene            3900..4727
                     /locus_tag="ACGI59_003603"
     CDS             3900..4727
                     /locus_tag="ACGI59_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HFG9741352.1"
                     /translation="MDLSKKVKILENASDGKSEFEILEFEKLEGASSPLMAMGLYFAR
                     EAGLKARQIKIKLNNSSIKTEAGALYYYKGNISSETKIGGVGGLFKKGISGALTNESM
                     MKPVYKGTGEVYLEPSFRHYLFMSLDNDSIIVDKGLFYCCSDTIEVKAFTQKNFSSAL
                     LGGEGFFQIELSGTGVIVLECVVPQSEIVEYELKNGEELKVDGNFAIARTSGVNFSVT
                     KSDKSLIGSALNGEGFLNTFTGNGTVWLAPTQVMYEKLSFGAMPTNDNMNNKVSKQK"
BASE COUNT         1809 a          839 c          569 g         2066 t
ORIGIN      
        1 taaattaact tgcaatataa aagcgcctct taatataggt gctataatgt tataaatttt
       61 taattatata ttccaatgaa cctttatcaa ccatatctaa attaaatata taatccatct
      121 tatcaaatgc atctcttaat ggaatacttg attcaagcca tcctaaccca tctgtaatcc
      181 aacaaaatgt aaaacctcca tcttttatat acttatctaa cttagtaaac tcatttgcaa
      241 cagattttaa cttagaacct ccaccattat aaaaattaac ttctactaaa acaatttttc
      301 cattattatt tatagcaaag tcatactttc ttttactctt atctgttgga acttctatag
      361 accatttctc acttattttt ttcactgtag cttgtgttaa ataactccaa ttattctttt
      421 cacacatatt ctttacatat ctttctacaa gagtttccat actacttcca gttctatttt
      481 ttctagcatt cgtatcaagc ccaacctcta ctccaaacac ataatctact aaatttttta
      541 tatttttttg ttttaataaa tctaaaaact ttattttttt aagaaattct gtagctaatt
      601 cgctttcttc atcacttata ctctttctct tcttaaaatt aaacagttta tatttaaaca
      661 tatcctcatc tgatggatct agtattttga attcattatc tctactcgct attactattg
      721 gtatcacact aagaacttct ggatactctt ttaataaatt tttagtttcg ttttctatat
      781 cttcttttcc tataagatag tttaagatat ttaactgaac ctctatcttt ctagtatttt
      841 ccaaagcttt attccagtta acataataat cccagctagc tatagtacct tttaaatttt
      901 caagtaattc cttaaatatt tcatccttac tcttattttt atgaatattc ttaaccaatt
      961 ccatttgatt ccccacctct tcataattag taattagcaa ttcagttata aaacctcttc
     1021 cattcccctt agaatttatg aatcttttag catgtactct ttttatatta aaatctgaat
     1081 acaaatcatc aaagaaagta tcttcttcat ctaaaacttt tggatcagaa ttacttaaca
     1141 tcaattttgt atctatttta tctagaactt tataaaattt acttaactca atttgactat
     1201 catcattaaa attacctttg ttataggaat taaatcctcc tatagttact ggtctatatg
     1261 gtggatctag atacacaaaa caatttttat ctattatatt ttctagattt aaataattct
     1321 tatttaatat ttttactttt agctctcctc gagaatctct tttatttaaa agcatggata
     1381 atttttcaag ttcattttta tcaaaaaaac taggattttt atatcttcca aaaggtacat
     1441 taaattcacc tcgagaattt tctctatata atccattaaa acatgtttta tttaaaaata
     1501 taaaatatga agcttgcttt atacaatctt cattttctat atttttttca tttgtaagta
     1561 tattaaatct atctctaata tcatagtaca tcttttctct atctgaagaa ccttcttcgt
     1621 aagatatgta ttgttctttt aaattagtta gttctgttat taactcttcc aaatcatctc
     1681 tgactttaat atataaatta attaacttac tatttatatc attaattaca tacttgttga
     1741 aaatgtcctc ttctaacaaa tatagaaata gtgcacctgc acctacaaaa ggttctatat
     1801 aagtcatgga actaaaatca caacctgtag gcatttgttt tcttagaata tcaagtagct
     1861 gtgttttacc tccagcccat tttaaaattg gtttcatgca acacctctct cagttatatt
     1921 gttatataat tatataaaat aagattctaa aatacaagta ctcatcagaa cactatattt
     1981 atatcaaatc tttccaaaat ctaagtatta ttttatccta acacttttca aaacctctaa
     2041 ataatataca atattctcta atatttactt actaaaaatt taaatctatc tttaagtata
     2101 acctaaaaca gattataagt tacgtagttt ttaatataaa atatacttct acaagtaaag
     2161 ctttaaatat caattactta tttcataatt aatgactatg taccctcaaa aaaacctcat
     2221 tttctatggt tggggtacgg ttggtggatt tcatcaccaa aataaaaaat actagtcaat
     2281 aaagactagt atttccaagt atttcatcat tcttttttca aaattggtgg agacgagggg
     2341 agtcgaaccc ctgtccgcaa atcttttcct tgctgcatct acatgcttag ccaacttcac
     2401 taatatctca cctcacatag cgccaagtgg caggctctat gcttagccag tcccaattaa
     2461 tctacttcag gtcgagacac ccctaaagta gttccctgca taatgacgtc cagtcttagt
     2521 cggcaggagg actaagaggg acgagcagca actaggctgc tatcgcgaaa ttatcgtctg
     2581 cgtttatttt aaagttcccc agtttttacg tgacccagag taacacgaca tgcagcctca
     2641 agttccaaac ccacgtcgaa acctttacgc ccccttgagt agtttatagt ccagtagact
     2701 tttcgcaaac tttataacca tttttattaa atttttaatg tatatataat ataatacttt
     2761 taagaataaa aatcaatgtt atgttctaga taataaagaa ttttattcta aaaaatacag
     2821 ttttatctag tttaatactt acctgtcatt tctctttcta tacgtctttg tgcatctttc
     2881 ttagccatat cttgacgttt atcatatagt tttttacctt ttgctaaggc aatctctact
     2941 ttaactttac cgttttttag atacaaacta agtggtataa gtgaataccc ctttatagta
     3001 gttgctccaa taagctttct tatttcatgc ttatgaagta atagttttct aactcttaaa
     3061 gaatcaacat tgaatatatt tccttgctca tatggactta tatgaacttg ttttaaaaat
     3121 acttcgctat tatctacaga tgcaaatcca tcacttaggt taactttgcc ttgtcttatt
     3181 gattttactt ctgttccttt taattcaatt ccacattcat aagtttcttc tataaaatat
     3241 tcatgtcttg ctcttttgtt tgttgctaat atttttttac cagccatata aatctcacct
     3301 tctttgctag aaatttattc cataaatatt agaaatttac taaatattta atgttgccaa
     3361 attatttgac taacttgtat gataacatat ttaggcattt tagtcaatat ttcactgata
     3421 tattaagtta gttaggctat atataattaa gtaatataaa tgtatgaaaa tagctaaaaa
     3481 actacttcga atatacttaa tactaaacta actctaacca gtaatactaa tttataaatt
     3541 tcaaaacaaa aatattacat attataaatc ttctatcttc tatcttctat cttctatctt
     3601 ctatcttcta tcttctatct tctatcttct atcttctatc ttctatcttc tatcttctaa
     3661 ataattttaa catgcccata aaatataata cccacaaatg ataaataata ctcaaaatgt
     3721 ctattgaaat aactatacat ccttaaataa cttaacttaa atctaaacat ttcaaaaact
     3781 tcttaaaaat atgtaaaaat ttgatgataa agtagcgatt ttttcaaaaa aaatgtaata
     3841 tagagttata tcttataata ttttagagat aaattttatc atagtgggga gaaatttgta
     3901 tggatttaag caaaaaagta aaaattttag aaaatgcatc agatggcaaa agcgaatttg
     3961 agatattaga atttgaaaaa cttgagggag cttctagccc attaatggca atgggattat
     4021 attttgctag agaagctggt ttgaaagcta gacaaattaa gataaaatta aataattcat
     4081 caataaaaac agaagctggt gcattatact attataaagg aaatatatct tctgaaacta
     4141 agattggtgg tgttggaggt ctttttaaaa aaggaatatc aggagcactt actaatgaat
     4201 caatgatgaa acctgtatac aaaggtactg gagaagttta tttggaacct tcttttagac
     4261 attatttatt tatgagtctt gataatgatt ctataattgt tgataaagga ttattctatt
     4321 gttgctctga tacaatagaa gtaaaagcat ttacacaaaa gaacttctca tctgctttac
     4381 ttggtggtga aggcttcttc caaatagaat taagtggtac tggtgtaatc gttctggaat
     4441 gtgttgtacc tcaaagtgaa attgtagagt atgaattgaa aaatggtgaa gaattaaagg
     4501 tagatggtaa ctttgcaata gcaagaacat ctggtgttaa tttttctgtt acaaaatcag
     4561 ataaatcttt aattggttct gctttaaatg gtgaaggttt cttaaataca tttactggta
     4621 atggtacagt ttggctagct ccaactcaag taatgtatga aaaactaagt tttggagcaa
     4681 tgcctactaa tgataatatg aataataaag tttctaagca aaaatagttg ttttataaat
     4741 atttattaaa tcattattga ttttataaag tcactataaa ttttattaga ttattattat
     4801 aaaatcagag tgtggaaatg tttatgtgta ttctttaaat aaaaatttcc acattctgta
     4861 aggttattat acttgatata taaaattcaa tttaggaaca tagtctaaca tttataaaca
     4921 tattaaggta atgatttctc ttggtaatta ttctcatatc ttattctttg attatattta
     4981 tcttattcct agcccttatg tcatattata tttcttatct tattacttat aattagcatt
     5041 aacttatcta aaatcttgta tttttttaat agtaaagttt acatctatat taaaaagttt
     5101 acatctatat taatttcata aaacaatcaa ggtagtcaag agattcaact ttacttgatt
     5161 ttaaaaattt aagtgaattt gttcttatac acatcttttt aaaattatat ctgtagaaaa
     5221 tatcatttag agctataatt aaattaactt attaaatttt agaaaattta ttaaaaaata
     5281 taa
//
LOCUS       DAYAPA010000185         5185 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.190, whole genome shotgun sequence.
ACCESSION   DAYAPA010000185 DAYAPA010000000
VERSION     DAYAPA010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5185
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.190"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(37..1221)
                     /locus_tag="ACGI59_003604"
     CDS             complement(37..1221)
                     /locus_tag="ACGI59_003604"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFG9741353.1"
                     /translation="MLSKRLNFITPSYTIGISSKVKEMESNGVKVINLSIGEPDFNVP
                     NNAKSYGIDSLNKDYTKYDLVPGLKILREEICKKLIEENNCNYSIDEIVVSSGAKNSI
                     TNTLLALTDEGDEVLLPKPYWVSYPEMVKLVNAVPVFIDTKKENGFKLTKDELEKSIT
                     DKTKILVINNPSNPTGSVYTKDELIEIVDVCIQNKIYILADEIYEKICYTGEFTSIAS
                     LSEEAKDITITINGFSKSAAMTGLRLGYTASNKTIAKAMSSIQGHLISHPSLTAQYIA
                     YGALKDCSIDIDNMVKTYKSRRDLIKSKLDSIDNVGYVNPNGAFYIFIDLSKVSEKFE
                     YKDSFSIEFCNQFLEEYNVAVVPGIAFGMDKYIRISYACSENTFLSGLDKLKEFVYKI
                     MA"
     gene            1594..3945
                     /locus_tag="ACGI59_003605"
     CDS             1594..3945
                     /locus_tag="ACGI59_003605"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside triphosphate reductase"
                     /protein_id="HFG9741354.1"
                     /translation="MVEFVKKRDGRVIPFNEDRITRAIFLAATNVAEREGIVPDYKLS
                     EQLTQEVIKFLNHKYSESVPSVEDIQDSVVKVLIETGHAKTSEEYIIYRTERSRIRNS
                     KTRLMKAIEEITFEDAEDADIKRENANINGNTAMGTMLQYGSTVSKEFCKTHILKPEH
                     SFAHDNGDIHIHDMDFLNMGTLTCCQIDVKKLFNGGFSTGHGFLREPQDIISYGALAA
                     IAIQSNQNDQHGGQSIPFFDYGLAEGVYKTFKKFYIGNLAKALKLFKGIENNDVIKNI
                     VYNTEKETNQKVGLKRDELYLNLEKEKLIQTFDIDDELVNKMQNFAFEESYRETDKKT
                     YQSMEAFIHNLNTMHSRAGAQVPFSSVNFGTDTSEEGRMVTKNLLLSQERGLGNGETP
                     IFPILIFKVKEGINLNPEDPNYDLFKLSCRVSAKRLFPNFSFLDAPFNAKYYKKGEPD
                     TEATYMGCRTRVLSNVCGSETVSGRGNISFTTVNLPRLGIKHGIINNEKANLDGFFEE
                     LDEKINLIIEQLLERLEVQGNKKMKNFPFLMGQGVWKGSDDLGPEDTLKEVIKQGTLT
                     IGFIGLAECLIALIGKHHGESKEAQELGLKIVSHMRHKMDEATDKYKLNFSLMGTPAE
                     GLSGRFTKIDKKVYGEIKGITDKEYYTNSFHVPVYYNISAYDKIEIEAPYHELTNAGH
                     ITYVELDGDPSDNLEAFETVIKAMKDLGIGYGSINHPVDRDPICGFSGVITSNICPVC
                     GRNEDESDIKFERIRRITGYLVGTVDRFNNAKKAEVRDRVKHR"
     gene            3967..4506
                     /gene="nrdG"
                     /locus_tag="ACGI59_003606"
     CDS             3967..4506
                     /gene="nrdG"
                     /locus_tag="ACGI59_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HFG9741355.1"
                     /translation="MKIRMSSTISYDSIVDGPGLRMVIWTQGCIHNCKECHNPQTHDL
                     CGGFYMDTEEIINKVKSLKLQKGITLSGGEPFLQPEPLEEIAREAKINGLDVWSYTGF
                     TFEQLLDKKNRAYFKNLNLLKQIDILVDGKFIAEKKDISLKFRGSSNQRIIDVQKSLK
                     YKKVFLVEQYMKDDLSIAE"
BASE COUNT         2033 a          585 c          839 g         1728 t
ORIGIN      
        1 taatacatta tatctaatta ctattcatct aaattattat gccattattt tatatacaaa
       61 ttcttttaat ttatctaatc cagataagaa agtattttca ctgcaagcat aggatatacg
      121 aatatattta tccataccaa atgctatgcc aggaacaact gcaacattat attcttctaa
      181 aaattgatta caaaattcta tagaaaaact atctttatat tcaaatttct cagatacttt
      241 agaaagatct ataaatatat agaatgcgcc atttggattc acatatccta cattatcaat
      301 agagtctagt tttgatttaa ttaaatctct tcttgattta tatgttttaa ccatattgtc
      361 tatgtcaatt gagcaatctt tcaaggctcc ataggctatg tattgtgctg ttagacttgg
      421 gtgagatata agatgtcctt gtatagaact cattgcttta gcaatcgttt tattagatgc
      481 agtatatcct aatcttaatc cagtcattgc agctgactta gaaaaaccat ttatagttat
      541 tgttatatct ttagcttctt cgcttaatga tgcaattgat gtaaattcac cagtgtagca
      601 tattttttca tatatttcat ctgctaaaat atatatttta ttttgtatgc atacatctac
      661 tatttctatt aattcatctt ttgtataaac actaccagta ggatttgaag gattgtttat
      721 tacaagaatt tttgttttat ctgttataga tttttcaagt tcatcttttg ttaacttaaa
      781 tccattttct tttttagtat caataaaaac tggaacagca ttaactaact taaccatttc
      841 aggataacta acccaataag gctttggtaa taagacttca tctccttcgt ctgttaaagc
      901 tagtaaagta tttgttattg agttttttgc tccacttgat acaactattt catctattga
      961 atagttacag ttattttctt caataagttt tttacagatt tcctctctaa gtatttttaa
     1021 tcctggaact aaatcatatt tagtgtaatc tttatttaaa gaatcaatac cataagattt
     1081 tgcgttatta ggtacattaa aatcgggttc tcctatacta agatttatta ctttaactcc
     1141 attactttcc atttctttaa ctttagaact aatacctatt gtgtaggatg gtgttataaa
     1201 gtttagtctt tttgataaca taaaatgcct ccttttaaat tattatttat attatacgac
     1261 aaatttaaag aatttacata aggaaatttg tagatatata cattaatata tataatagcc
     1321 tacacttttt gcctaaaatt agtgtaaaaa aaagagaaaa aaattttaac tttcaaagtt
     1381 gttagaaacg ctactgttca aaatatccac atagtatttg caatgatttg cgcaagtttg
     1441 actattatgt acaaatttgg taatcttaat atagcaatta ttaagataat ccctttaact
     1501 tagttgaagg ggattttttt agacttaaca tactacatat agtggtatgg ggattaataa
     1561 aatacaatat atagtaacag gaggtttttt taattggttg aattcgtaaa aaaaagagat
     1621 ggaagagtaa ttccatttaa tgaagataga ataacaagag ctatattttt agcagcaacg
     1681 aatgttgcag aaagagaagg aatagtacct gattataaat tatctgagca attaactcaa
     1741 gaagtaataa agtttttaaa tcataaatac tcagaatcag ttccaagtgt tgaagatata
     1801 caagattctg tagttaaggt tcttatagaa actggccatg caaaaactag tgaagaatat
     1861 ataatataca gaactgaaag aagtagaata agaaattcaa aaacaagatt gatgaaggca
     1921 atagaggaaa ttacctttga agatgcagaa gatgctgata taaaaagaga aaatgcaaat
     1981 attaatggta atacagcaat gggaactatg ttacaatatg gaagcactgt atctaaagaa
     2041 ttttgtaaaa cacatatatt aaaaccagag cattcttttg ctcatgataa tggagatata
     2101 cacatacatg atatggattt tttaaatatg ggaacattaa cttgttgtca aatagatgtt
     2161 aaaaaactat tcaatggtgg attttcaaca ggacatggct ttttaagaga gccacaagat
     2221 ataataagtt atggtgcatt agctgcaatt gctatacaaa gcaatcaaaa cgatcaacat
     2281 ggaggtcaaa gtataccatt tttcgattat ggattggctg aaggtgtata taagacattt
     2341 aagaaattct acataggaaa tttagctaaa gcattgaagt tatttaaagg aattgaaaat
     2401 aatgatgtga taaagaacat agtatataat acagagaaag aaacaaatca aaaagttggt
     2461 cttaagagag atgaattgta tctaaactta gaaaaagaga aattaataca gacttttgat
     2521 atagatgatg aacttgtaaa taagatgcaa aactttgcat ttgaagaatc gtatagagaa
     2581 actgataaaa aaacatatca atctatggaa gcatttatac acaatctaaa tacaatgcac
     2641 tctagagcag gtgcacaggt accattttca agtgtaaact tcggaacaga tacttctgaa
     2701 gaaggaagaa tggtaactaa gaatcttcta ttatctcaag aaagaggttt aggtaatgga
     2761 gagacaccaa tttttccaat tttaatattt aaggtcaaag aaggcataaa tttaaatcct
     2821 gaagacccta attatgattt atttaaatta tcatgcagag tatcagctaa aagactattc
     2881 cctaatttta gctttttaga tgcgcctttt aatgcaaaat attataaaaa aggtgaacca
     2941 gatacagagg ctacatatat gggttgtaga acaagagtac ttagtaatgt gtgtggttca
     3001 gaaacagtta gtggtagagg aaacatatct tttactacag taaatttacc aagattgggt
     3061 ataaaacatg gtattataaa taatgaaaag gctaatttag atggtttctt tgaagaatta
     3121 gatgagaaga taaacttaat tatagaacaa ttattggagc gccttgaggt acaaggaaat
     3181 aaaaagatga agaattttcc attcttaatg gggcaaggtg tatggaaagg ttctgatgat
     3241 ttaggaccag aagatacact aaaagaagta ataaaacaag gaacattgac aattggattt
     3301 ataggtttag cagagtgcct tattgctctt ataggtaaac atcatggaga aagtaaagaa
     3361 gctcaagaat taggattaaa aatagtgtca catatgagac ataaaatgga tgaagcaacg
     3421 gacaaatata aattaaattt ctccttaatg ggtactccag cagaaggtct ttcaggtagg
     3481 tttactaaaa tagataaaaa agtttatgga gaaattaaag gtatcacaga taaagaatat
     3541 tatactaatt cattccatgt accagtttat tacaatataa gtgcatatga taaaattgag
     3601 atagaagctc cataccatga attgactaat gcaggtcata taacatatgt agagttagat
     3661 ggagatccat ctgataattt agaagctttt gaaactgtta taaaagctat gaaagatttg
     3721 ggtataggat atggaagtat aaatcatcct gtagatagag acccaatatg cggtttttca
     3781 ggtgtaataa caagtaatat atgccctgta tgtggaagaa atgaagatga aagtgacatc
     3841 aaattcgaaa gaataagaag aataacagga tacttagttg gtacagttga tagatttaat
     3901 aatgctaaaa aagcagaagt aagagataga gtaaaacata gatagtttta tttgggggaa
     3961 taagttatga aaataagaat gtcatctact ataagttatg acagcatagt tgatggacca
     4021 ggattaagaa tggtaatatg gactcaagga tgtattcata attgtaaaga gtgccataat
     4081 cctcagacac atgatttatg tggaggattt tatatggata cagaagaaat tataaataaa
     4141 gtcaaatcat tgaaattaca aaaaggcatt acgctatcag gaggagaacc atttttacaa
     4201 ccagaaccat tagaagaaat agcaagagaa gctaaaataa atgggttaga tgtatggtca
     4261 tatactggat ttacgtttga acaactgtta gataaaaaaa atagagcgta ttttaaaaat
     4321 ttaaatttac taaagcaaat tgatatttta gtggatggaa aatttatagc tgaaaaaaaa
     4381 gatataagct taaagtttag agggtcatca aatcaaagga taattgatgt tcaaaaaagt
     4441 ttgaaatata aaaaagtgtt tttggttgag caatatatga aggatgattt atctatagca
     4501 gaataaaata taaataaaca ggattaaata tatgcatata tttaatcctg tttattttca
     4561 tataatattt caagcttgta taatgtaaca attaaaccaa aagactcatt gtatatttga
     4621 aattaagttt tttatagttt ataggtgttt tatgtggatt acgaatgata tagtattgat
     4681 acattattta atagattctt atgccaagaa acaattaatt tatagaaatt cttaattgtt
     4741 acaatataaa tatctagata agttataaga tactgataaa taatttataa agtttattaa
     4801 tctaatattg tgtgtgaata ttaagtggtc aagtagtcta aaaaatgaaa aagatttaaa
     4861 aaagtgtttt aactaaacac ataagtgtta aattcatgta taagtatagg ttagatacat
     4921 aagaaataat tatgtaatat ctatctttgt aaaaaaatat taaattttgt aatttgtgga
     4981 taaatatgtt tataatgtgg ataatattta atgtaataag ttaaagcaat atgaatcaac
     5041 tgtttataat gtggataata ttataaatgt atgaataatt gcagtattta aaaacaaaaa
     5101 aagttaaaaa agttgttgac tctaaaagaa aaagttggta tagtaatact tgtcctcaag
     5161 aaaagggcaa acaaataaag cataa
//
LOCUS       DAYAPA010000186         5167 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.191, whole genome shotgun sequence.
ACCESSION   DAYAPA010000186 DAYAPA010000000
VERSION     DAYAPA010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5167
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.191"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(229..2508)
                     /locus_tag="ACGI59_003607"
     CDS             complement(229..2508)
                     /locus_tag="ACGI59_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFG9741356.1"
                     /translation="MLSDAKNNRYGLSIIAVISILVITISCLIYGSRLNKTLGEETNQ
                     YLSEIANQSVNVLKKQINGDIKLLESISIFIEGEESFEVDNILSILKRQAINSSFKRM
                     GVILPNGTAYTTDGYIEDFSKRDYFLKSMQGEVVITGKLKDVIEDDKNTNSNINVYSV
                     PIYKDNEVKGVIFATHSTKLYEKILSVPTFNGKGYSYITRSNGDIVLNPSSKNANSTK
                     ENLFSYIGRSDSISKSSINNMKSNIKNFKSGSLSYSMFGKGYYLSYAPIGINDWYLFS
                     EVPKTAISEKSYAIIKLTLSACVVLIIIFTALTIYILFIHKKSKEKLEEFAFKDNVTG
                     IGNSNKFNLEGGKFLSSHEKKNLVLIYFDIDKFKLVNDRFGYEEGDRVLKEIAEIIKN
                     IFKEQSVFSRISNDNFAIIFEKKNNRESIIEICELIRKKLSMIKTSLGVELNLIPSIG
                     VYFVEEGETNISTCLDKAMIAKTTVKRKYLKVYEIYAENLKETLIEERDIEQEMHDAL
                     KNEQFKVYLQPKVDLSTTKIVGAEALVRWQHPKKGLISPGVFIPIFEKNGFITELDMF
                     VFTQVCKNFKRWENENFPTFPISINLSRVHLENPGFISELERITKEYEIEPNLIEIEL
                     TESAIFDNTKILFKIMQTLKSVGFKISMDDFGSGYSSLNMLKDMPIDVLKLDRQFFIT
                     VGNAKKSQIVVSSIVQMAKQLDIKVVSEGVETVEQAEFLRFIGCDMAQGFLFARPMPI
                     EEYEKLILDRYSLNRKETN"
     gene            complement(2779..4233)
                     /locus_tag="ACGI59_003608"
     CDS             complement(2779..4233)
                     /locus_tag="ACGI59_003608"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HFG9741357.1"
                     /translation="MKLNDIIQGLDIINVKGELNIDINNVQYDSRKVTKGTLFICIKG
                     FVSDGHKYIKDAIEKGASAFLVEEDVAIKGCTFIKVKDTRKDMAKVADNFYNHPSQKF
                     NVIGVTGTNGKTSITTILNEILTLNKNKVGLIGTIKIFDGEKDIVSNSTTPESIDLQY
                     HFNNMLDNGCDYCAMEVSSHSLALNRVDETDFKLGIFTNLTPDHLDFHKDLEDYRKAK
                     EKLFFKTTMANIINIDDEGGKKIYENIKGINVPCYTYGVDTKADFMARDIKSDSDGVS
                     YRLITPSYEEVIFIPVPGMFTVYNTLAVIAACYVLGIPKPIYKEGLRLSNGVSGRFET
                     VPNDKGISVIVDYAHTPDALENVLKTTQQFAEGKIISVFGCGGDRDTEKRPLMGAIGQ
                     KYSDLCIITSDNPRTEEPEAIIKDILEGIDKKKENYHVVVDREQAIAEAISMAKKDDV
                     VIITGKGHETYQIIGKVKHHFDDKEVANECLSKM"
     gene            4397..>5167
                     /locus_tag="ACGI59_003609"
     CDS             4397..>5167
                     /locus_tag="ACGI59_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFG9741358.1"
                     /translation="MDNKIGYLNDNFKIFNIRDKKDIQFEYHNHDFNKIIIFINGNVT
                     YFVDGIPYKLKPWDILFISNNEIHKPVIDSNVFYERIAIWISPEFMKKNSDASSDLEK
                     CFKIATENKCNRLRLNMHDIESIKSFIEQINISESNTEFGNEILRNTLFIQLMIFLNR
                     IFLKNENMESTEDVFYDETIKKVLNYINSNIDDKLSIDSISSEFFISKYYLMRKFKSH
                     TGTSIHNYIVQKRLILAKSLITNGHLMSEVCNKCGFNDY"
BASE COUNT         1753 a          816 c          550 g         2048 t
ORIGIN      
        1 tattattact atctaaagta ttttgctata atcccatata ctcttacctc aaaaacattc
       61 aaatgtaaaa aatactacat attaataaac tttgcccaat aattcctaaa ataatatcct
      121 aacaatgatt tatgttttta taatagtata tttttacata aaaaagtgaa cattgattaa
      181 caaagttcac ttttttataa atttttatgt tataattcac aattttattt aatttgtttc
      241 ttttctattt aaactatatc tatctaatat taatttttca tattcttcta ttggcattgg
      301 tctagcaaat aaaaatcctt gggccatatc acaacctata aaccttaaaa actctgcttg
      361 ctctacagtt tcaacacctt ctgaaacaac ttttatatca agttgctttg ccatttgaac
      421 aatacttgaa actacaattt gactcttttt tgcatttccc actgttataa agaattgcct
      481 atctaatttc aatacatcaa ttggcatatc tttaagcata tttaaagaag aataaccaga
      541 accaaaatca tccatagaaa ttttaaatcc aacagatttc aaagtttgca ttattttaaa
      601 taatattttt gtattgtcaa atattgcact ttctgtcaat tcaatctcaa ttaaatttgg
      661 ttcaatttca tattcttttg ttattctttc aagctcagaa ataaagcctg gattctcaag
      721 atgtactctt gataagttta ttgatatagg aaatgtgggg aaattttcat tttcccaacg
      781 tttaaaattt ttacaaactt gggtaaatac aaacatatcc aattctgtta taaaaccatt
      841 tttctcaaaa ataggaataa atacacctgg acttattaga ccttttttgg gatgttgcca
      901 acgtacaagt gcttcagcac ctacaatttt agtagttgat aaatcaacct taggctgtaa
      961 ataaacttta aattgttcat tttttaaagc atcatgcatt tcttgttcaa tatcacgttc
     1021 ttctatgaga gtttctttta aattttcagc ataaatctca taaactttta aatattttct
     1081 ttttactgta gtttttgcaa tcatagcttt gtcaagacag gtagaaatat ttgtttctcc
     1141 ctcttcaaca aaatatacac ctatggatgg aattaaattg agttctactc ccaaacttgt
     1201 ttttatcatt gacaattttt tacgtataag ttcacatatt tctataattg actctctatt
     1261 atttttcttt tcaaatatta tagcaaaatt gtcatttgaa attcttgaaa atacagattg
     1321 ctctttaaat atattcttaa ttatctctgc tatttccttt aatactctat ctccttcttc
     1381 atacccaaac ctatcattta ctagcttaaa cttatctata tcaaaataaa ttaaaactag
     1441 attctttttc tcatgagagg ataaaaattt tcccccttca agattaaatt tatttgaatt
     1501 gccaatacct gtaacgttat ctttaaaagc aaattcttcc aatttttcct tactcttttt
     1561 atgtataaat agtatataaa ttgttaatgc cgtaaagatt ataatcaaga ctacacatgc
     1621 tgataatgta agttttataa tagcatatga tttctcagat atagcagttt taggaacttc
     1681 agaaaaaaga taccaatcat tgattcctat tggggcataa cttaaataat atccttttcc
     1741 aaacatacta taacttaatg aacctgattt aaagtttttt atatttgact tcatattgtt
     1801 gatactagat tttgatattg aatcacttct gccaatgtaa gaaaataaat tttctttagt
     1861 agagttggcg tttttactgc ttggatttaa aacaatatca ccattacttc tagttatata
     1921 agaatatcct tttccattaa atgtaggtac agaaagtatt ttttcataca acttcgtact
     1981 atgtgtggca aatataactc ctttaacctc attatcttta taaattggta cagaatatac
     2041 attaatatta ctattggtat ttttatcatc ttctattaca tcttttaatt ttcctgttat
     2101 aaccacttct ccttgcattg attttaagaa ataatctctt ttagagaagt cttctatata
     2161 accatctgta gtgtatgctg taccatttgg taaaataact cccattcttt taaatgaact
     2221 attaatagct tgtcttttta gtatagaaag gatattatct acttcaaaag actcttctcc
     2281 ttcaataaat attgaaattg actctagtaa ctttatatct ccattaattt gtttttttaa
     2341 cacattgacg ctttgatttg ctatttctga aagatattga ttcgtttctt cacctaatgt
     2401 cttattcaac cttgaaccat aaatcaaaca ggaaattgta attactaaga tacttatgac
     2461 agcaattatg gacaaaccat acctattatt ttttgcatca cttaacatta tgcttcctgc
     2521 ctttctcgaa tctatgtatg tataatcatt atcttatgac aattattgta aacattttat
     2581 aataatactt atttttccat atttactgta attatacaaa attataaact ttattacaac
     2641 aataaattaa ttaatataga tattagtgtt tacattattg cattatctat tttacaatta
     2701 tttacatata aaaaaatgta atatcaaagt gaagcttgat attacatttt tgttcttgta
     2761 ttttgtttaa tttttatgct acatcttact taaacactca tttgcaactt ctttatcatc
     2821 aaaatgatgc ttaactttac ctataatttg ataagtttca tgtcccttac ctgttataat
     2881 tacaacatca tccttttttg ccatacttat agcttctgct atagcttgtt ctctatcaac
     2941 aacaacatgg taattttctt ttttcttgtc aattccttca agtatatctt ttattattgc
     3001 ctctggttct tcagttcttg gattatcaga agttataata cataaatcag aatatttttg
     3061 tccaattgct cccatcaaag gtctcttttc agtatctcta tctccaccac acccaaacac
     3121 agaaattatt tttccttctg caaattgttg agttgtcttt agtacatttt caagtgcgtc
     3181 aggagtatga gcatagtcta caattacgct tataccttta tcatttggaa ctgtttcaaa
     3241 ccttcctgat actccattag aaagtctaag gccttcttta tatattggtt taggtatgcc
     3301 aagtacatag caagcagcta taactgctaa agtattatat actgtaaaca tccctggaac
     3361 tggtataaat attacttctt catatgaagg tgttattaat ctataagata ctccatccga
     3421 atctgatttt atatctctag ccataaaatc agccttagta tcaacaccat aagtgtaaca
     3481 aggtacattt atacctttta tattttcata tatctttttt ccaccttcat catctatgtt
     3541 tattatattt gccatagtag tcttaaaaaa taatttttct tttgcttttc tataatcctc
     3601 taaatcttta tgaaaatcta aatggtctgg tgttaaattt gtaaatatgc ctagtttaaa
     3661 gtctgtttca tcaactctat ttaatgccaa tgaatgtgat gaaacttcca ttgcacagta
     3721 gtcacatcca ttatccaaca tattattaaa atgatattgt aaatctatac tttctggagt
     3781 tgttgaattg gaaacaatgt ctttttctcc atcaaaaatt tttatagttc ctataagtcc
     3841 aactttattc ttatttaaag ttaatatctc atttagtata gtagttatac tagtttttcc
     3901 attagtacct gtaactccta taacgttaaa tttttgagat ggatgattgt aaaaattatc
     3961 agctactttt gccatatctt ttcttgtatc ttttaccttt ataaatgtac atccttttat
     4021 tgctacatct tcttcaacaa gaaatgcact tgctcccttt tctatggcat cttttatata
     4081 cttatgacca tctgacacaa atccttttat acatataaat aatgtacctt ttgtaacctt
     4141 tcttgaatca tattgtacat tattaatatc tatatttaat tcacccttga cattgattat
     4201 atccaacccc tgtataatat catttaattt cataaatgct ttcctccctt taaactcttt
     4261 ctgctaaaat tacactacat ataatattat attaagaatt ttttactatg tgaatggtta
     4321 aaaatgcttg aaacattgca aaaaatgctc tataattttc tataatataa ctgaggaaag
     4381 gtcttctata aaaactatgg ataataaaat cgggtactta aacgataact ttaaaatatt
     4441 taatattaga gataaaaaag atatacaatt tgaataccat aatcatgatt ttaataagat
     4501 aattatattt ataaatggaa atgtaactta ttttgttgat ggtattccct ataaactaaa
     4561 gccttgggat atactcttta tcagcaataa tgaaatccat aaaccagtta tagattcaaa
     4621 tgtcttttat gagagaattg caatatggat tagcccagaa tttatgaaaa aaaatagtga
     4681 tgctagtagt gacttagaaa aatgttttaa aatagctaca gagaataaat gcaatagatt
     4741 gcgtttaaat atgcatgata ttgaatctat taagagtttt attgagcaaa taaatatttc
     4801 cgaatccaat actgaatttg gaaatgaaat attgagaaat acattattta tacaacttat
     4861 gatattctta aacagaattt tcctaaaaaa tgaaaatatg gaatcaacag aagatgtttt
     4921 ttatgatgaa actattaaga aagtattaaa ctatataaat tcaaatatag atgataagct
     4981 ttcaatagat agcatatcgt ctgaattttt tataagtaag tattatctta tgagaaaatt
     5041 taaatctcat actggaacgt ctatacacaa ttatatagtt caaaaaagat taattttggc
     5101 aaagtcacta ataaccaatg gtcaccttat gagtgaagtt tgtaataaat gtggattcaa
     5161 cgattat
//
LOCUS       DAYAPA010000187         5156 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.192, whole genome shotgun sequence.
ACCESSION   DAYAPA010000187 DAYAPA010000000
VERSION     DAYAPA010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5156
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.192"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            750..980
                     /locus_tag="ACGI59_003610"
     CDS             750..980
                     /locus_tag="ACGI59_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFG9741359.1"
                     /translation="MSNKKKKYANILANECVACGSCIKACPRSAISVPCGISAKINRD
                     LCVGCGICEKICPASVIEIITILKEEEGKCHE"
     gene            973..1554
                     /locus_tag="ACGI59_003611"
     CDS             973..1554
                     /locus_tag="ACGI59_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFG9741360.1"
                     /translation="MNNKKHWYDYLWIFSSIYLILGFVNIIFAWIGLICFFVPLAISI
                     VKGNKAYCNKYCGRGQLFNLLGNKLKLSRNRDIPKFIRSKWFRYGFLTFFMVMFANML
                     FSTYLVFEGSRNFKEVVTLLWTIKMPWQWAYHGTLVSPWVVQFAFGFYSVMLTSTVLG
                     LITMILFKPRSWCVYCPMGTMTQMICKAKSKSV"
     gene            complement(2133..2810)
                     /locus_tag="ACGI59_003612"
     CDS             complement(2133..2810)
                     /locus_tag="ACGI59_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFG9741361.1"
                     /translation="MISQNEIDILTHSLPFWDKLTDLQKELLISSANTSHYKKGNPVH
                     CGDSDCIGILIIKSGTVRTYILSDEGREVTLFRLDDGDVCILSASCILKTITFDVYVD
                     AETDCDIIQISSSVFAKLSTENIHAELFSYKLATERFSDVMWAMQQILFMSFDKRLAS
                     FLIDEIAKNGSSTINMTHEQIAKYMGSAREVVSRMLKYFAREGIVSLSRGGIKVLDKD
                     KLRSLTL"
     gene            complement(2999..4363)
                     /locus_tag="ACGI59_003613"
     CDS             complement(2999..4363)
                     /locus_tag="ACGI59_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFG9741362.1"
                     /translation="MKESNKRIELLGSAPISKALLSMGLPTMIGMMITALYNLVDAYF
                     VGGLGTSQMGAISVAFPLGQLVVGLGLLFGNGAASYVARLLGHGDNEKAGKVVSTSIY
                     SSIIVGTLVIIVIMIFLNPILKLLGATKSIMPYAISYASIYVVSSIFNIFNVTMTNIV
                     TSEGAAKTTMCAMLLGALLNMILDPIFIYTFGLGVRGASIATAISQIASTLVYVGYIL
                     SKKSIFSFRINECCFSKEIFSEILKIGIPTLIFQLLTSLAITLTNMEATKYGDSVIAG
                     MGAVTRITSLGSLVVFGFIKGFQPIAGYNFGSKNYDRLHIAIKTSILWTTVFCVVLGL
                     ILILFPKSIISQFTSGDILLIENGSKALRANGFSFMLFGFYTVYSTLFLALGKAKEGG
                     FLGMCRQGVCFIPIILIIPVFYGLNGILYAQPIADLMAAIIAGFMSIRLHKELNLLKI
                     NGMH"
     gene            4491..5012
                     /locus_tag="ACGI59_003614"
     CDS             4491..5012
                     /locus_tag="ACGI59_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFG9741363.1"
                     /translation="MATIDLIVLGIVKQQPKSAYDIQKAVEYRNISRWVKISTPSIYK
                     KVIQLEQKGYIESKTVKNGKMPEKAVYSLTELGETHFLDLMFDTSSKPINILLDFNAV
                     IVNLNSLTPEKQKLCLNNIEENVQELKKTIEENISIKTEVPDTAKAVLEQQLVLAQAL
                     ETWINSLKDNMGN"
BASE COUNT         1958 a          673 c          825 g         1700 t
ORIGIN      
        1 tgtaactaaa gataaacttc aatatgagct taacctatga ttaatagagt aagaatgtaa
       61 atagagataa acttcaatat gagtttaact tgtggctaat agagtaagag tgtaactaaa
      121 gataaacttc aatatgggtt taacttgtgg ttaatagagt aagaatgtaa atagagataa
      181 acttcaatat gagcttaact tgtgattaat agagtaagag tgtaaataga gataaacttc
      241 aatatgggat taacttgtga ttaatagagt aagagtgtaa ctaaagataa acttcaatat
      301 gagcttaacc tatgattaat agagtaagaa tgtaaataga gataaacttc aatatgggtt
      361 taacctgtgg ttaatagagt agggtgtaac taaagataaa cttcaatatg agtttaactt
      421 gtggctaata gaataagatt gtgaataaag ttatagtttt agatgggtat taacctgtta
      481 ctaatagaat atgattaggt gtaaaattat agagctgcat aagaaaatat tatagatata
      541 ttaaatatta gataataaat atttatatag atacacaaaa gaaaaggcga tatgaagcta
      601 aacttaatga cttcatatcg ttttttattt gaaactcctt tgtatttaga gtgtgatttt
      661 taatattgag gttttatgtg attaagtcac agataaaaag taagtatctt gatataattc
      721 ttatagatta ataatatagg gaggttatta tgtctaataa aaaaaagaag tatgcaaata
      781 ttttagcaaa tgagtgcgtt gcttgtggta gctgtataaa agcatgtcca agaagtgcta
      841 tctcagttcc atgtggcatt agtgcaaaaa ttaatagaga tttatgtgtt ggatgtggga
      901 tttgtgaaaa gatatgtcca gcatctgtaa ttgagattat aacaatatta aaagaggaag
      961 aaggtaagtg tcatgaataa taaaaaacat tggtatgact atttatggat tttttcttca
     1021 atatatttga tattgggatt tgtaaatatt attttcgctt ggattgggct aatttgtttc
     1081 tttgttccac tagctatttc tattgttaag ggaaataaag cttactgtaa taagtattgt
     1141 ggaagagggc aactgtttaa tttgcttgga aataaattga agctgtcaag aaatagagat
     1201 attcctaaat ttattagaag taagtggttt agatatgggt ttttgacatt ttttatggta
     1261 atgtttgcca atatgctttt ttcaacttat ttagtgtttg aaggaagtag aaattttaaa
     1321 gaagtagtga ctttgttgtg gactataaaa atgccttggc aatgggcgta tcatggaact
     1381 ttagtttcac cttgggtggt acagtttgca tttggttttt atagtgttat gcttacatca
     1441 actgttcttg gtttgattac tatgatattg tttaaacctc gttcatggtg tgtttattgc
     1501 cctatgggta ctatgactca gatgatatgt aaggcaaaga gtaagtctgt atagaaaggc
     1561 cttatgtgat atttacagtt ttgttcaagt agagatgagt agtaggtttg aatatatatg
     1621 acaaatatat gagtataggg gcatagattt agtttgtgta aggtaaactg atttatgctt
     1681 cttttttatg tcttagatta ttggctaagt ggaaacaatg aatttagata ttaagaatta
     1741 ttcagttatt tgaagaaaat agaataataa ttaacattat attaatggat attcattaca
     1801 tataagccag ttaatagcaa gatttattta atatgatata ggtcaagtat ttaaactaaa
     1861 taaggtcatg acaattaata taagtataga tataaaaaga ttagaagtta taacttataa
     1921 aaggtattct atataaatag tatggggaaa atagtcaaat taacttatta gaaacaaaaa
     1981 atatacataa aaaataaatg taaatatcaa ataagatact atatcaaaat agatataagt
     2041 atctttttta ttattgaaat tatgaaaata tatttgattt tacacaaaaa agagtgcctc
     2101 atgaactaaa aagttcattt tgagacacac ttttacagtg tcaaagaacg aagtttatcc
     2161 ttatccaata cctttatacc accacgagat aaagacacaa taccttctct agcaaaatat
     2221 ttcaacattc tcgatacaac ttctctggca ctacccatat acttagcaat ttgctcatga
     2281 gtcatattta tagtgctact gccattttta gctatctcat caattaaaaa agatgccaaa
     2341 cgcttatcaa aactcataaa aagaatttgt tgcatagccc acataacatc agaaaaacgt
     2401 tcagtagcta atttatatga aaataactct gcatgaatat tttctgtaga taattttgca
     2461 aaaacagagg aactaatctg tataatatcg cagtccgttt cagcatctac atagacatca
     2521 aaagttattg tttttagaat acaggaagct gataaaatac atacatctcc atcatcaagt
     2581 cggaacagtg taacctctcg accttcatct gaaagtatgt atgtacgcac tgttccagat
     2641 tttataatca aaataccaat acagtcacta tcaccacaat gtactggatt cccttttttg
     2701 taatgcgagg tatttgctga agaaattaat aattcttttt gtaagtcagt aagtttatcc
     2761 caaaaaggca agctatgtgt aagtatatca atttcatttt gtgaaatcat acaatatctc
     2821 ctttcttaaa ttgttataat cccatgatac aagtgaatag ataaattgac aagtagtttg
     2881 ttaagaggat tatttagtca gatagtaata gtatgattta tgtagaaaaa atatgataat
     2941 atatattctt gctattaatg agaaagtaat atgttttgat gatagtaggt ttgtataact
     3001 aatgcatacc gtttattttt aaaagattaa gttccttatg taaccttata gacataaaac
     3061 cagcaattat agcagccatc aaatcagcta ttggttgtgc atataagata ccatttaacc
     3121 catagaatac aggaataatc aaaattatag ggataaaaca gacaccttgt ctacacatac
     3181 ctaaaaatcc accttccttt gcttttccta gtgcgaggaa taatgtagaa tacactgtat
     3241 agaatccaaa tagcataaat gaaaacccat ttgccctaag agctttacta ccattttcta
     3301 ttaataaaat gtcaccactt gtaaattgtg aaattataga ttttgggaag agtatcaata
     3361 taagaccaag cactacacaa aatactgtag tccataatat ggaagttttt attgctatat
     3421 gtaatctatc gtagttcttt gaaccaaaat tataaccagc aattggttgg aaacctttta
     3481 taaaaccaaa tacaacaaga ctacctaaag acgttattcg tgttacagca cccattccag
     3541 ctatcacaga atcaccatac tttgtagcct ccatattagt taatgtaata gcaagactag
     3601 tcaataattg aaatattaag gtaggtatgc caatttttag gatttctgaa aaaatttctt
     3661 ttgaaaaaca gcattcgtta attctaaaac taaatatact tttcttgctt aaaatatatc
     3721 ctacataaac aagtgttgaa gcaatctgtg agatagctgt tgcaatagaa gcacccctta
     3781 cccccagacc aaaagtataa atgaatatcg ggtctaaaat catattaagc aaagcaccta
     3841 ataacatagc acacatagtt gttttagcag ctccttcact tgtgacaata ttagtcatag
     3901 tgacattgaa tatattaaaa atagatgaca cgacataaat gctagcataa gatattgcat
     3961 aaggcataat actttttgta gctcctaaca attttaaaat tggattgaga aaaatcataa
     4021 tcacaataat aactaatgta cccactataa tactactgta aatagatgta cttaccactt
     4081 ttcctgcttt ttcattatca ccatggccta aaagtctggc aacatatgaa gcagctccat
     4141 ttccaaacaa taatcctaat cccacaacaa gctgacctaa tggaaatgca acggatatag
     4201 ctcccatttg acttgttcca agtccaccca caaagtatgc atcaaccaaa ttgtataatg
     4261 cagttatcat catacctatc atggtaggta atcccataga taacaatgct tttgagattg
     4321 gggcagaccc taataattca attcttttgt tactttcttt catacttaac actcctcttc
     4381 tattgacatt aagtctaaat tataatacta taaattatag taagtatgga aaatagtact
     4441 ataatttata gtatttgtca atagtaagaa aactaaagga gatgattttt atggctacaa
     4501 tagatttaat tgtattggga atagtaaagc aacaacccaa gagtgcttat gatattcaaa
     4561 aggcagtgga atacaggaat atatcacgtt gggttaaaat aagtacacca tcaatttata
     4621 aaaaagtaat tcaattagaa caaaagggat atattgaaag taaaactgtg aagaatggaa
     4681 aaatgccaga aaaagcagtt tattcattaa ctgaattagg tgagacacat tttttagatt
     4741 taatgtttga tacatctagc aaaccaataa atattctttt agattttaat gctgttatag
     4801 taaatttaaa tagccttaca ccagaaaaac agaaattatg tctcaataat attgaagaga
     4861 atgttcaaga gttaaagaaa actattgagg aaaatataag tataaagaca gaagtaccag
     4921 acacagcaaa agcagtgttg gagcaacagt tggtgctagc acaagcattg gaaacctgga
     4981 taaattcttt aaaagataat atgggaaatt agaattagag aagctaactt aataatcatt
     5041 aaacaaaaac cagcatattt aataatgaga tattagaaaa aataaaacaa atgatataaa
     5101 ctgaaaagta tatttaaaat aaagctcact taattgaggg agaatgttaa actttt
//
LOCUS       DAYAPA010000188         5025 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.193, whole genome shotgun sequence.
ACCESSION   DAYAPA010000188 DAYAPA010000000
VERSION     DAYAPA010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5025
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.193"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(196..717)
                     /locus_tag="ACGI59_003615"
     CDS             complement(196..717)
                     /locus_tag="ACGI59_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFG9741364.1"
                     /translation="MIPLLILGLLKQNPNSYGYELLALMEKKHYKYIVNFTKGSFYYN
                     LQQLEEKKFIEKVENKQSNKNRETHNYIITELGCQEFEKLMYKYGSKTEYINLSFYAT
                     LLFANDYKNEKLIELLETQIKQTQVRISLLNESIENDKTNSHYFNRMLENSRSHHLVN
                     IEWFEKLLNDIRD"
     gene            complement(769..1617)
                     /locus_tag="ACGI59_003616"
     CDS             complement(769..1617)
                     /locus_tag="ACGI59_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003442793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFG9741365.1"
                     /translation="MIKSFLMLGQSNMAGRGFISEVTPIYNERIQMLRNGRWQMMTEP
                     INYDRPVSGVSLAASFADAWCCENQEDRIGLIPCAEGGSSLDEWNIDGILFKHAISEA
                     KFAIQSSELTGILWHQGENDSNNGNYKFYYKKLLSIIETLRKELNIPDIPIIIGGLGD
                     FLGKVGFGKSCTEYVFINQELQKFAFEQDNCYFVTATGLTSNPDGIHIDAISQRKFGL
                     RYFEAFHKKKHIMEALANESELIIPSNSKTYTKTEKIYINSMDLALGKISYDEFESKL
                     IKLNDN"
     gene            complement(1767..2048)
                     /locus_tag="ACGI59_003617"
     CDS             complement(1767..2048)
                     /locus_tag="ACGI59_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9741366.1"
                     /translation="MPNIEKDLDGTTTITYLNDDIGVPTDENGYKISMTILHGKTICH
                     VVSPETILGRKFTLEEKQEKDTIIYGAIEKIMVDRQLQIKNSNKEVASH"
     gene            2433..3287
                     /locus_tag="ACGI59_003618"
     CDS             2433..3287
                     /locus_tag="ACGI59_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFG9741367.1"
                     /translation="MEWIERLNKAINYIEENITKEIEYEQVAKIACCSTYHFQRMFAY
                     MADVPLSEYIRRRRMSLAAVELQNDDKKIIDVALKYGYSSPTAFNRAFKSIHGVAPSV
                     IKKREITTLKAFPPISFKISIKGAEEMNYRIEKKEAFRIVGVSQPLHNELEKNFEIVP
                     QMWQKVALDGTLQKLIPMMDSQLQGVLGISICNNSEEWKYFISVSSTKSIDNTLEEYT
                     VPAFTWAIFSGEGQCPQAIQELEKRIVTEWLPTSGYEYDNGPDIELYLNPDPQNAKFE
                     VWIPVVKK"
     gene            3486..4991
                     /locus_tag="ACGI59_003619"
     CDS             3486..4991
                     /locus_tag="ACGI59_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFG9741368.1"
                     /translation="MNVAIYLRKSRADEEAEHRGEFETLSRHKTTLLKLAREQNLNVI
                     EIKEELVSGESIEYRPKMLELLGEIKNNNYDAVLVMDIDRLGRGNMQDQGLILDTFKK
                     SKTKIITPRKTYDLNDEFDEEYSEFEAFMARKELKLISRRMQRGKIKSIEEGKFIAQA
                     PPYGYDIKYLNNKERVLVINEDKATVVKIIFDMYLKGDGACKISNYLNELGYKTTTGR
                     TWYEKGIRDIIKNKVYCGYIVWNKVERKRNSSRTRGIEEQIEVKGKHEPIISEDIWNK
                     AQNIRKKRINSPTPNNKITNPLAGIVKCGYCNHTLIAKSNTTSNGYEKYMVCTNCKNK
                     ATKLYILENKILEVLNKWLVNYRLSLKDEKDNSKPENTLEHTLINLNKELDTLEKQKE
                     RLHDFLEQGIYDSNTFGERLKILLSRIENAREAINKTNIDIEMQKERNTVKTQIVPQL
                     EKILSEYTEEKEPYLKNILLKSVINYAVYKKEPNQKSDDFSLILYPNIPRD"
BASE COUNT         1887 a          655 c          689 g         1794 t
ORIGIN      
        1 cttttcatct taagtgtctg taattcaaca atgaacaact ttatatttat aaattttact
       61 tacatagtat aagtttcttt taacatctta tataacaaaa agacagatat tcaattgaaa
      121 attatctgcc ttatttttaa tctatatatc atacatttat ataaaatata tatgtatgtt
      181 tttttattat taaaatcaat ctctaatatc atttagcaac ttctcaaacc attcaatatt
      241 aactaaatgg tgagaacgag aattttctag cattctatta aaataatgtg aatttgtttt
      301 gtcattttct atggattcat ttaatagaga aattcttact tgagtttgct taatttgcgt
      361 ttccaataat tctattaact tttcattttt ataatcattt gcaaataata gtgttgcgta
      421 aaatgataga tttatatact ctgttttaga accatactta tacattaatt tttcaaattc
      481 ctggcatcca agttcagtaa ttatgtagtt atgagtttct ctatttttat tagattgttt
      541 attttcaact ttttcaataa actttttttc ttcaagttgc tgtaaattat aataaaaaga
      601 acctttagtg aaatttacta tgtacttgta atgcttcttt tccattaatg ctaataactc
      661 atatccataa gaatttggat tttgttttaa taatcctaaa attaataatg gtatcaaaaa
      721 atcacctctt tcaatctata tttattagta tattaatgaa tattttatct aattatcatt
      781 aagtttaata agtttagatt caaactcatc atatgatatc tttcctagtg ccaagtccat
      841 actatttata tatatttttt ctgtttttgt atatgtcttg gaattactcg gtattatcaa
      901 ctcactttca ttagctaatg cttccataat atgctttttc ttatgaaaag cttcaaaata
      961 acgtaatcca aattttcttt gagatattgc atcaatatga ataccatcag gattagatgt
     1021 aagacctgtt gctgtaacaa agtaacaatt atcttgttca aaagcaaatt tctgtaactc
     1081 ttgattgata aatacatact cagtacaact ttttccaaaa cctacttttc ctaagaagtc
     1141 tcccaatcca ccaataataa ttggtatatc tggaatattt aattcttttc taagagtttc
     1201 aataatagaa agtaattttt tataataaaa tttatagttg ccattattac tgtcattttc
     1261 tccctgatgc cataaaattc ctgtcaattc actgctttgt atggcaaatt tagcttcact
     1321 tatagcatgt ttaaaaagta taccatctat attccattca tctaaggaac tgcctccctc
     1381 agcacaagga attagcccaa ttctatcctc ttgattttca cagcaccatg catctgcaaa
     1441 tgatgcagca agacttaccc cagaaactgg tctgtcataa ttaataggtt ctgtcatcat
     1501 ttgccatctt ccattacgta acatctgtat tctttcatta taaattggag tcacttcact
     1561 tatgaatcct cttccagcca tattggactg tcctaacatt aaaaatgatt taatcattat
     1621 tattattatc ctttcttaaa ttcttattgt atttattttt aatagtagtc taattagact
     1681 atctatgttt atattatagt ctaattagac tataagtcaa taatattata aaaaatactt
     1741 gtcctacttt acttattaaa gaagtgctaa tgactagcaa cttctttgtt tgaatttttt
     1801 atttgtaatt gtctgtctac cattattttt tctattgctc catatattat tgtatctttt
     1861 tcttgttttt cttctaatgt gaactttctt cctaagattg tttcaggaga tactacatgg
     1921 cagattgtct ttccatgtag tattgtcata gatattttat atccattctc gtctgttgga
     1981 acacctatat cgtcgttgag gtatgttata gtagttgtac catctaagtc tttttctatg
     2041 tttggcattt aaatcccctc ctctttatat tttatgtatc ttaaaaattg tcctattctc
     2101 aatcattatt tataattgaa taactaagtg ttgcttgatt aagggtaaac ttcaaaaata
     2161 gttaactata aataaaatgt tatatataat taaatgtcta agctaaaata ttataaaaat
     2221 ttcataataa attaaaactg ttatcttata taaaaattag atactaattt gtgatatact
     2281 ttaatgtgaa aaacaatact atttgttcat ttttgtagtt aattacagac tttctcatgt
     2341 tcattcttta ttaagctagt taattagtgc atagaaagag aggattaact attttaaaat
     2401 agatattata taaattacaa ggagctgatt ttatggaatg gattgaaaga ttaaacaaag
     2461 caattaacta tattgaagaa aacataacaa aagaaattga gtatgaacaa gtggcaaaaa
     2521 tagcttgttg ttctacatat cattttcaaa gaatgtttgc ttatatggca gatgtaccac
     2581 tttctgaata tattcgtcgt agacggatgt ctttagcagc tgtagaatta caaaatgatg
     2641 ataaaaagat aattgatgtg gctttaaaat atggatactc ttctccaaca gcattcaatc
     2701 gtgcatttaa aagtattcat ggtgttgccc catcagttat aaaaaaaaga gaaataacaa
     2761 ctttaaaagc ctttcctcca atcagtttta aaattagtat taaaggagcc gaagaaatga
     2821 attatagaat tgaaaagaaa gaagcatttc gtatagttgg tgtatctcag ccacttcata
     2881 atgaactaga aaagaatttt gaaattgttc cccaaatgtg gcaaaaggtt gctttagatg
     2941 gaacgcttca aaaattaatc cctatgatgg atagtcaact tcaaggtgta ttaggtataa
     3001 gtatttgtaa taattcagaa gaatggaagt attttatatc tgtttcaagt acaaaatcta
     3061 tagataatac attagaagaa tacactgttc cagcttttac ttgggcaata ttttctggag
     3121 aaggacaatg tccacaagca atacaagaac tagaaaagcg tattgtaaca gaatggttac
     3181 ctacttctgg ttatgaatat gataatggtc ctgatattga actgtactta aatccagacc
     3241 ctcaaaatgc taaatttgaa gtatggatac ctgtagtaaa aaaatagttt aaaattaaaa
     3301 aatgaagaaa gcataaaatt aatagtataa atatttactt agattacaag cttttaaata
     3361 tactataaaa tttactgtat aaaaataaac ctaaaaaata tatcttgtat tttgaaggtt
     3421 tatttttgtg ttttaaatat aatcaatctg ttactgaata taatatattc aaggggtgtg
     3481 atttaatgaa tgtggcaatt tatttacgca aaagcagagc tgatgaagaa gcagaacata
     3541 gaggtgaatt tgaaactcta agtagacata agacaactct tttaaaacta gcaagagaac
     3601 aaaatttaaa tgtcattgaa attaaagaag aacttgtttc tggggaaagt atagaatata
     3661 gacctaagat gctagaatta ctaggtgaaa ttaaaaataa taactatgat gctgttttag
     3721 tgatggatat tgaccgtttg ggtcgtggta atatgcaaga ccaagggtta atactagata
     3781 catttaaaaa atctaaaacg aaaattatta ctcctagaaa aacgtatgat ttaaatgatg
     3841 agtttgatga ggagtattct gaatttgaag cttttatggc tcgtaaggaa ttaaaattaa
     3901 tatctagacg tatgcaaaga ggaaaaatca aaagtattga agagggtaaa tttatagctc
     3961 aagctcctcc ttatgggtat gatattaaat atttaaataa taaagaaaga gtacttgtaa
     4021 taaatgaaga taaagcaact gttgttaaaa taatctttga tatgtattta aaaggagatg
     4081 gtgcatgtaa aatatccaat tatttaaacg aacttggtta taagaccaca acaggtagaa
     4141 cttggtatga aaagggtata agagatatta taaaaaacaa agtgtattgt ggatatatag
     4201 tatggaataa agtcgagagg aaaagaaatt ctagtagaac aagaggaatt gaggaacaaa
     4261 ttgaggtaaa aggaaagcat gaaccaataa taagtgaaga catatggaat aaggcacaaa
     4321 atataagaaa aaaacgtatt aattccccta ctccaaacaa taaaataaca aatccattgg
     4381 ctggaattgt aaaatgtgga tactgtaatc atactttaat agccaaatct aatactacat
     4441 caaatggata tgagaaatat atggtttgta ctaattgtaa aaataaagct actaaactat
     4501 acatattaga aaataaaata ttagaggtct taaataaatg gttagtaaat tatagactat
     4561 ctttaaaaga tgaaaaagac aactctaaac ctgaaaatac tttagaacat accttgataa
     4621 atttaaataa agaattggat actttagaaa aacaaaaaga acgactacat gattttttag
     4681 aacaaggaat ttatgacagt aatacatttg gggaacgttt aaaaatactc ttatctcgta
     4741 tagaaaatgc aagagaagct attaataaaa caaatataga tattgaaatg caaaaggaaa
     4801 gaaatacagt taaaacacaa attgttcctc aacttgaaaa aattttaagt gaatatacag
     4861 aggaaaaaga accttatcta aaaaatatat tgcttaaaag tgtaataaat tacgcagtct
     4921 ataaaaaaga acctaatcaa aaatctgatg atttttcttt aatactttac cctaatatcc
     4981 caagagatta attatatgtt ttaaatgtta taaatagact gatta
//
LOCUS       DAYAPA010000189         4943 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.194, whole genome shotgun sequence.
ACCESSION   DAYAPA010000189 DAYAPA010000000
VERSION     DAYAPA010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4943
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.194"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            669..4811
                     /locus_tag="ACGI59_003620"
     CDS             669..4811
                     /locus_tag="ACGI59_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HFG9741369.1"
                     /translation="MATVSGQIVFDRNRSATIDSGDSGIANIPVVLQDIATGVRLVVL
                     TDASGNYVFTNVPNGNYRIVEAFGTTGGVPTPGNFATAIVGPVPIAEMPPISSVTSPP
                     IGSTNLDGTTPNTLLITVAGSDITNQNILNGPVIYTPIENILDSCVSVSNINLITDAD
                     NGTFGFFPPGTPSNTGPEIEPYPNVTPDFTYVVPDPTKFTPLDGEYTVQNIMTNAMSN
                     VIGAWWRIADHTTGNETGRMMVVNGFNPGAVFFKSTVQVTPNTNYLFSTWILNLFKVT
                     GYPPPQLGVRIFDQNNNILYQATLGAQIPVNTNAPEWKEVGTVINSQNNNQLTVEFFS
                     EGESAIGNDYAIDDVSLREIAVPTFTPIKTSNTETANVGDIITYTVTLDNTCDSPLTN
                     VFFRDLIPNGLEFIPGTVTVDGVSVPGVDPNTGFPLPDIGGGTGVEVTFDVVVESIPN
                     PNPTNNIATIDYSYTPVEGGIPNDFSVDSNPVPVEVISADIEVTKLSEPTIVNPGEEL
                     IYTIKVVNNGPFPSENVVLTDDVPASIVNPEYSLDGGVTFQPWTGSLNIGTLEVGETR
                     VIIIRGIVNPSTVGIITNTAVVSSTTADPNLNNNTSTIETEVNLLADILVMKTAEPNS
                     AVPGTLLRYTIQVENLGPANAENVILNDDIPASIINPEYSLDGGASFQPWNGSLNIGT
                     LNSGISLTVLIQGTVSLNSSEYIVNTATVSSTTPDPDLSNNISTIITPVNPQAGISII
                     KVADEDVAVPGEEFVYTIEIFNEGPSNATNVVLTDDIPDVILNPEYSLDGGATFQPWT
                     GSLNIGTVAPGQLIRIIIKGLVSSTATGDITNTAEVSADVPEPVTDSSTVTTPIVPSA
                     DIEVIKTSNMDTAVPGETFSYTITVTNLGPSAAQSIVLTDDIPDVILNPEYSLDGGVT
                     FQPWNGNLSIGTLDAGEIRSIIIRGTVSQTAVGTIINTATISSTTPDPNPDNNTSTDE
                     TDISPLADISVIKGNEPVAIPGGRFIYGIEIANAGPSFAENVTLTDNIPASILNPEYS
                     IDNGVTFQPWNGSLNIGTLDAGEIRNIIIRGTVSQTAIGTIINTATVSSTTPDPNLNN
                     NISTSEAEVSSSADISVVKRSNQTVVVPGDVLDYTIEVRNAGPSTAQNVTLTDNIPAS
                     ILSPEYSIDNGVTFQPWNGSLSIGTLDAGEIRNIIIRGIVSQTAIGTIINTATVSSTT
                     PDPNPDNNTSTSEIDISSSADISVIKLANKTEACVGEQIDFVIVVSNAGPENAQNVTL
                     IDNVSDKLKKAVFSLDRGVSFQPWTGSLNIGTLPAGTLRVIILRGIIKSTCLDRLTNI
                     AEITSTTPDSNLNNNISRVQVEIKQCCCNDCCCNNCSNCCCNDCGCKDCCCNCCKNDF
                     WC"
BASE COUNT         1912 a          725 c          832 g         1474 t
ORIGIN      
        1 ttcagaaaaa aattttagtt tacataataa aaataaagtc aactaaaatt attgatttta
       61 gattaaagta ccggattaag catttaagta gattttaagc ttgatttgat tagaaaggta
      121 caaactatca ccgaaaaaaa ttaaacccat ttaaaaagca atataaaagc ttgttatttt
      181 tgagaaaaat agaccctcta aactcaatta gattaaaaat aaaatacaga aaattatatc
      241 tctaaatatc ttaaataatt aagtttaaat tttacgatat tttgtttgat gatttataat
      301 ataaaagcat actcttaatc ttcaaaattt aaaaagagta aaaaataaca aaaaaatcac
      361 tgacatttca ctgacaaatt gtaattttgt tattataaat acattgaaaa tacttgatta
      421 ttgaaaaatg gcataaaata atacatttta tgccatttgc tattatttga tagtttttaa
      481 cttatgacat aagatatttt ttaacatata caataaaata taaaaaaatt gatattgcaa
      541 ataaaaaaaa atacatatta gatgttatag aaaaaaagcg cacttatagg cttaaaatgt
      601 cacataaaag agttttaaag aataatatat tatgaacaca aattttgtaa tcacaaagga
      661 gtgataatat ggcaacagta tcaggacaaa tagtttttga tagaaataga agtgctacca
      721 ttgattctgg agactcagga atagccaata taccagttgt attacaagat atagcaactg
      781 gagttagact tgtggttctc acagatgcat caggaaatta tgtttttact aatgttccaa
      841 atggtaacta tagaattgta gaagcttttg gaactacagg aggagtacca acaccaggaa
      901 attttgcaac agcgatagta ggtccagttc caattgcaga aatgccacct attagttctg
      961 tgacaagtcc tcctattggt tcaacaaact tagatggtac aacacctaac acactattaa
     1021 taacagtagc aggttcagat ataacaaatc aaaatatatt aaatgggcct gtgatttata
     1081 cacctataga gaatatattg gattcttgcg tatctgtatc taatataaac cttattacag
     1141 atgcagataa cggaacattc ggtttttttc caccaggtac accaagtaat acaggacctg
     1201 aaatagaacc ttacccaaat gtgacgccag attttacgta tgttgtacca gacccaacta
     1261 agtttacacc tttagatgga gaatatacag tacaaaatat tatgacaaat gcaatgagta
     1321 atgttatagg agcttggtgg cgtattgctg accatactac aggaaatgaa acaggaagaa
     1381 tgatggtggt aaatggtttt aatcctggag ctgtattttt taaatcaaca gtacaagtta
     1441 cacctaacac taattattta tttagcactt ggattttaaa cctttttaag gtaacaggat
     1501 atccaccacc acagctaggt gtaagaatat ttgaccaaaa taataatata ctatatcaag
     1561 caacattagg agctcaaatt ccagttaata caaacgcacc agaatggaaa gaagtaggta
     1621 ctgtgattaa ttctcaaaat aataatcaac ttacagtaga attttttagt gaaggagaaa
     1681 gcgccattgg aaatgactat gctatagatg atgtttcttt aagagaaata gctgttccaa
     1741 cttttactcc aataaaaaca agtaacacag aaactgctaa tgtaggtgat ataatcacat
     1801 atacagttac attagataat acttgtgata gccctttaac taatgtattt tttagggatt
     1861 taattccaaa tggtttagag tttatacctg gaactgtaac ggttgatgga gtatctgtac
     1921 caggagtaga ccccaataca ggtttcccac ttccagatat tggaggagga acaggggttg
     1981 aagtaacttt tgatgttgtg gttgaaagta ttcctaaccc aaatcctact aataatatag
     2041 ctactataga ttactcctat acacctgtag aaggcggtat accaaatgat ttttcagtag
     2101 attctaatcc agttcctgtt gaagtaatct cagcagatat tgaagttaca aaattatctg
     2161 aacctactat agtgaatcca ggagaggaat taatatacac tataaaagta gtaaataatg
     2221 gaccattccc atctgaaaat gtggttttaa cagatgatgt accagcttct attgtaaatc
     2281 cagagtattc attagatgga ggagtaactt ttcaaccatg gactggaagt ttaaatatag
     2341 ggacattaga agtaggagag acaagagtta ttataattag gggtattgta aatccatcaa
     2401 cagtaggaat tataacaaat acagctgtag tatcttcaac tacagcagac ccaaacctta
     2461 ataataatac atctacaata gagacagaag tgaatttatt agcagatatt ttagtaatga
     2521 aaacagcaga acctaattca gctgttccag gtactttatt gagatatact attcaagtag
     2581 aaaacttagg accagcaaat gctgaaaatg taatattaaa tgatgatatt ccagcttcta
     2641 ttataaatcc agaatattca ttggatggag gagcaagttt tcaaccatgg aatggaagtc
     2701 ttaatatagg aactttaaac tctggaattt cattaacagt tttaattcaa ggaacagtta
     2761 gtttaaattc ttcagaatat atagtaaata cagcaacagt ttcttcaact acacctgacc
     2821 cagatttaag caacaatata tctaccatta ttacaccagt taacccacaa gcaggtattt
     2881 ctattataaa agtagctgac gaggatgtag ctgttccagg agaagaattt gtatatacaa
     2941 ttgaaatttt taatgaagga ccatctaatg ctacgaatgt agttttaaca gatgatatac
     3001 cagatgttat tttaaatcca gaatattcat tggatggagg agcaactttc caaccatgga
     3061 ctggaagtct taatataggt acagttgcac cagggcaatt aataagaatt atcataaagg
     3121 gattagtaag ttcaactgct acaggagata ttactaatac agcagaagta agtgcagatg
     3181 taccagaacc agttacggat tcatcaacag tgacaactcc tatagttcca tcagcagata
     3241 tagaagtaat aaaaactagc aatatggata cagcagttcc aggagaaact ttcagttata
     3301 ctataacagt tacaaattta ggaccatctg ctgcacaaag tatcgttttg acagatgata
     3361 taccagatgt tattttaaat ccagagtatt cattggatgg aggagtaact ttccaaccat
     3421 ggaatggaaa tcttagtata ggcactttgg atgctggaga aataagaagt attataataa
     3481 gaggaacagt gagccagaca gcagtaggta caattataaa tacagcaaca ataagttcaa
     3541 ctacacctga cccaaatcca gataataata cttcaacaga tgaaacagat ataagtccat
     3601 tagcagatat ttctgtgata aaaggcaacg aaccagttgc tataccaggg ggaagattta
     3661 tttatggaat tgaaattgca aatgcaggac catcttttgc agagaacgta actttaacag
     3721 ataatatacc agcttctatt ttaaatccag agtactcaat agataatgga gtgactttcc
     3781 aaccatggaa tggaagcctt aatataggta ctttagatgc tggagagata agaaatatca
     3841 taataagggg aacagtaagt caaacagcaa taggcacaat tataaataca gcaacagtaa
     3901 gttcaactac acctgaccca aatctaaata acaatatttc aacatcagaa gcagaggtaa
     3961 gttcatcagc agatatttct gtagtaaaac gaagcaatca gactgttgtt gtaccaggag
     4021 atgtactaga ctatacaatt gaggtgagaa atgcaggacc gtctactgct cagaacgtaa
     4081 ctttaacaga taatatacca gcttctattt taagtccaga atattcaata gataatggag
     4141 tgacttttca accatggaat ggaagtctta gtataggcac tttagatgct ggagagataa
     4201 gaaatattat aataagggga atagtaagtc aaacagcaat aggtacaatt ataaatacag
     4261 caacagtaag ttcaactaca cctgacccaa atccagataa taatacttca acatcagaaa
     4321 tagatataag ttcatcagca gatatttctg taataaaatt agcaaataaa acagaagcat
     4381 gtgtaggaga gcaaatagat tttgtaatag tagtatcaaa tgcaggacca gaaaatgctc
     4441 aaaatgttac tttaatagac aatgtttcag ataagcttaa gaaagcagta ttttcacttg
     4501 atagaggtgt aagtttccaa ccatggactg gaagtcttaa tataggtaca ctaccagctg
     4561 gaactttaag agtgattata ttaagaggaa ttataaaatc aacatgtctt gatagattaa
     4621 caaatattgc agaaattact tcaacaacac cagattcaaa ccttaacaat aatatatcaa
     4681 gagtgcaagt agaaataaaa caatgttgtt gtaatgattg ctgttgtaac aactgtagta
     4741 attgttgttg caatgattgt ggttgtaaag attgttgctg taattgttgt aaaaatgact
     4801 tttggtgttg attttataaa ataattttgt ataccttaaa ttaaactgaa ataaaaaata
     4861 aattcacata tgagtaaaat attttataaa tattaataaa ttttctatct ttgatatgga
     4921 ccacattcgt aatactagtt att
//
LOCUS       DAYAPA010000190         4868 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.195, whole genome shotgun sequence.
ACCESSION   DAYAPA010000190 DAYAPA010000000
VERSION     DAYAPA010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4868
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.195"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            190..1341
                     /locus_tag="ACGI59_003621"
     CDS             190..1341
                     /locus_tag="ACGI59_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFG9741370.1"
                     /translation="MEIYLDNSATTKPYQEVIDKMVYALNTEYGNPSSVHRKGVEVEK
                     AIKEVRQDIAKSLGAKEKEIYFTSGGTECNNTIIRGITSLNKKRKNHIISTNIEHPSV
                     LNTLKDLEEDGFEVTYLEVGKDGKINIEDLKNAIKSTTCLVSMMHVNNEIGTIQPIGE
                     VGKYLKGLKEKIYFHVDAIQSYGKINFRPSKYNIDFMSVSAHKFHGPKGIGFMYIKEN
                     NRLKPMLTGGGQEIGIRSGTENVPGIYGLGEAVRILNKDLDAVISKVDNLKNILKNEI
                     IDNIEDIKINSPEDGVCHILNVSFRGTKGEVLLHYLEQKGIYVSTGSACSSKKKGSYV
                     LNAIGLTNEEINGTIRFSLSDMNTEEEMLEAVKVLKESICDLRSIMKRK"
     gene            1387..2562
                     /gene="thiI"
                     /locus_tag="ACGI59_003622"
     CDS             1387..2562
                     /gene="thiI"
                     /locus_tag="ACGI59_003622"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFG9741371.1"
                     /translation="MVYNILIVKYGEIGVKGKNRYIFENRLIRNIRNMLKPIGRFNVY
                     KEYGRIYVDLEDYDYEEVIEEVRKVFGIVGVCPGVRAKKDYDTLKEIALKMLEEKIEA
                     GYKTFKVESRRGDKSFGLTSQEMSMDIGGYLLSKVGDRINVDVRNPEVKIKCEYREFH
                     TMVYSDTIPGYGGLPLGTNGRAMSLLSGGIDSPVATWMVAKRGMEVEAVHFHSYPFTS
                     ERSQEKVKDLAKILAKYCGRVRLHKVNILEIQKAIGENCNEEESTILSRRFMMRIAQR
                     LSEKRHCDALITGESIGQVASQTIQGLTCTNAVVDLPVFRPLIAMDKSDIVDIAKKIG
                     TFETSIVPEEDCCSVFSPRKPVTKPRLEKIEKSETALDIEKLVQDAIDKIEVEDIEF"
     gene            2718..3521
                     /locus_tag="ACGI59_003623"
     CDS             2718..3521
                     /locus_tag="ACGI59_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HFG9741372.1"
                     /translation="MNKKGFTLIELLVVISIIGILVIVAVPALFRNIEKSKAVTCLSN
                     RENIKTQIVIAMAEESSKGKNEVMKEVLENKDGKYFETEPKCKSGGIYSATFDDGYDG
                     ITGIESIAKVYVTCTKHPDGVEMARDIHQSMKDLIASFAQDPSIIPGASKGNDDFRKY
                     LLDNKYKNGWPTIPDEFKAKYGLSKDTLYIQPYAYNPTKSDATVVVFANNKTGGNWYT
                     SLVYDYDEGRWYKGKNGISVAGRSWDVDTDSVKSVKTEIHSKEGWGPLN"
     gene            3764..4405
                     /locus_tag="ACGI59_003624"
     CDS             3764..4405
                     /locus_tag="ACGI59_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HFG9741373.1"
                     /translation="MNNNFQDNQTINLIQSRRSIRKFTTEQISDEQVNTLLHCAFAAP
                     SGCNKQPWHITVVQDQKLLKEISDDTLSRIHEVSNVEINKNFKLFYGAPTVLFISYDE
                     SSSWAPYDIGILTGNITTAAQALGLGSCIIGMVRGLFTPVEQGDIEGLVSVLDKEDVK
                     ESESIKMKFDTNKKYRELLDIPEGYSVPFGIAVGIPDGNLPNAREVVYKVSRV"
BASE COUNT         1955 a          461 c          932 g         1520 t
ORIGIN      
        1 tagggtatag tataagtata ttatatttta ctataaaaat ttgtaataaa gtgaaaaaac
       61 tagcttttca tgaaataata ttttataatt taagattgtc tttttaataa agattaacaa
      121 aaaaaatact ataattagtg gtataatagt tagagttgaa gattttaaat aaaggtaggt
      181 gtaattaagt tggaaatata cttagacaat agcgcaacta caaaacctta tcaagaagtt
      241 atagataaaa tggtttatgc acttaatacg gaatatggaa atccttcttc tgtccataga
      301 aaaggtgtag aagttgaaaa agctataaaa gaagtaagac aagatatagc taaatctcta
      361 ggagcgaaag aaaaggagat ttattttaca tcaggtggta cagagtgtaa taatactata
      421 ataagaggta ttactagttt aaataaaaaa agaaaaaatc acataatatc tactaacata
      481 gaacatcctt ctgttttaaa tacattaaaa gatttagaag aagatggttt tgaagtaact
      541 tatttagagg tcggaaaaga tgggaaaata aatatagagg atttaaaaaa tgcgataaag
      601 tcaactacat gcttagtaag catgatgcat gttaacaatg aaataggaac tatacagcca
      661 ataggtgaag ttggaaagta tttgaagggt ttaaaagaaa aaatttattt ccatgttgat
      721 gctattcagt catatggaaa aattaacttt agaccatcta aatacaatat agattttatg
      781 agtgtaagtg cacataaatt tcatggtcca aagggaattg gatttatgta tataaaagaa
      841 aataatcgcc ttaagcctat gttaacaggt ggtggtcagg aaattggaat taggtcggga
      901 acagaaaatg tacctggaat atatggtctt ggagaagctg ttagaatact aaacaaagat
      961 ttagatgctg ttatttcgaa agtagataac ttaaaaaata tattaaaaaa tgaaataatt
     1021 gataatatag aagatataaa aatcaattct ccagaagacg gagtatgtca tatattaaat
     1081 gtatcattta gagggacaaa aggagaagtt ttacttcatt acttagagca aaaaggtatt
     1141 tatgtatcaa ctggctctgc atgttcttct aaaaagaaag gtagttacgt attaaatgct
     1201 ataggtctta caaatgaaga aataaatgga acaataagat ttagtttatc tgatatgaat
     1261 acagaagaag aaatgttaga agctgtgaaa gttttgaaag aatcaatttg tgacttaagg
     1321 tcaataatga aaagaaaata aagaatactg tataaatagt gataataaat tagtttaaga
     1381 ggtgaaatgg tgtataatat attaatagta aagtatggag aaattggagt aaaaggaaag
     1441 aatagatata tatttgagaa taggcttata agaaatataa gaaatatgtt aaaaccaatt
     1501 ggaagattta atgtatataa agagtatgga agaatatatg ttgatttaga agactatgat
     1561 tatgaagaag taattgaaga agttagaaaa gtatttggga tagtgggtgt gtgccctgga
     1621 gtaagagcta aaaaagatta tgatacatta aaagaaatag ctttaaaaat gttagaagaa
     1681 aaaatagagg ctggatataa aacttttaag gtagagtcaa gaagaggaga taagtctttc
     1741 ggattgactt ctcaagaaat gagtatggat ataggtggat acttattatc aaaggtagga
     1801 gataggatta atgttgatgt aagaaatcca gaagttaaaa taaagtgcga atacagagaa
     1861 tttcatacta tggtctatag tgatactata cctggatatg gtggattgcc acttggaacg
     1921 aatggtagag ctatgtctct tttatcagga ggtatagata gcccagtagc tacatggatg
     1981 gttgcaaaaa gaggtatgga agtagaggca gttcactttc atagttatcc atttacaagt
     2041 gaaaggtcac aggaaaaagt taaggattta gctaaaatac tggcaaaata ctgtggtaga
     2101 gtaaggcttc ataaagttaa tatcttagaa atacaaaaag caattggcga gaattgtaat
     2161 gaagaagaat ctactattct ttcaagaaga tttatgatga ggatagcgca gagactttct
     2221 gaaaaaagac attgtgatgc tttaattaca ggtgaaagta ttgggcaggt tgcatctcaa
     2281 actatacaag gacttacttg tactaatgcc gttgtagact taccagtgtt tagaccactt
     2341 atagcaatgg ataaatcaga tatagttgat atagccaaaa aaatagggac ttttgaaaca
     2401 tctatagtgc ctgaagaaga ttgttgtagt gtattttctc ctagaaagcc cgtcactaaa
     2461 cctagattag agaaaataga aaaatctgaa acagcacttg acattgaaaa attagtacag
     2521 gatgctattg acaaaataga ggtagaggat attgagtttt agcatacaag ttatttaatt
     2581 actaaattga atattgagta tattagtagt tctgtaagta tattagtagt tctgttgaaa
     2641 ggatttatgt agacaattta ttaatatgta gtatttaaaa ataagtacta taattttatt
     2701 taatgggaat gattattatg aataaaaagg gttttacact aattgaattg ttagtagtta
     2761 tatctataat aggcatttta gttatagtag ctgttccagc attgtttaga aatatagaaa
     2821 aaagtaaagc agttacatgt ctttctaata gagaaaatat aaagactcaa attgttattg
     2881 caatggctga ggaatcaagt aaaggcaaga atgaagtcat gaaagaggta ttagaaaaca
     2941 aagatggtaa gtactttgaa acagaaccaa agtgtaagtc aggtggaata tattcagcaa
     3001 cgtttgatga tggttatgat ggaataactg gaatagaaag cattgcaaaa gtgtatgtta
     3061 cttgtacaaa acatccagat ggtgttgaaa tggctaggga tatacatcaa agtatgaaag
     3121 atttgattgc atcatttgca caagaccctt ctataatacc aggagcctca aagggtaatg
     3181 atgattttag aaaatattta ttagacaata aatataaaaa tgggtggcct acaattccag
     3241 atgaatttaa ggcaaaatat ggattaagta aggatacact atatatacaa ccatatgcat
     3301 ataatcctac taaatctgat gctactgtag ttgtatttgc aaataataag actgggggta
     3361 attggtatac ttccctagtt tacgattatg atgaaggtag atggtataaa ggtaaaaatg
     3421 gtatttctgt tgcaggtagg tcatgggatg ttgacacaga tagtgttaag tctgtaaaaa
     3481 cagagattca ttctaaagag ggatggggtc ctttaaatta atatatcatg ttttattaat
     3541 ctggtattga ttaaactata gaaaagagca gtttctatag atgacgaata ccagattttc
     3601 atttatatat ataattgaga tatattaaaa gtgtttatat ttttaagtct tcacatacaa
     3661 gctaaaaata attataataa tattgtaaag aaatttaatt ataagattaa ttttctgaag
     3721 atttagtatt tgtactaaga aatacataaa aaggggaata gatatgaata ataattttca
     3781 agacaaccaa acaataaatt taatacaaag tcgtagaagt atacgaaaat ttacgacaga
     3841 acaaattagt gatgagcaag taaacacatt attacactgt gcatttgcag caccttcagg
     3901 atgtaataaa cagccttggc acataactgt tgtgcaagac cagaaacttt taaaagagat
     3961 tagtgatgat actttatcta ggattcatga ggtgagtaat gttgagataa ataaaaactt
     4021 taagttattt tatggtgctc caaccgtttt atttatttct tatgatgaaa gtagttcatg
     4081 ggctccatat gatataggaa tacttacagg gaatataaca acagctgcac aagcactagg
     4141 gcttggaagt tgtataatag gaatggttag aggtttattt acacctgttg aacaagggga
     4201 tattgaaggt cttgtgagtg tattggataa agaagatgta aaagaatcag agtcaataaa
     4261 gatgaagttt gatacaaata aaaaatacag agaactactt gatataccag aagggtattc
     4321 tgtaccattt ggtattgctg taggtattcc agatggaaat ttaccaaatg ctagagaggt
     4381 agtttacaaa gtttccagag tttaggataa agtattgcaa gaaccaatca gttacttgaa
     4441 gaattaaatc agtaatatac aaataaattg atggaagagg aagctattta aaaagtagtt
     4501 tagatttttc gttgtaaaat aattaaataa agaataaggt gataagtatt ttatttatta
     4561 tcttattctt ttttattttt gttttgtaga atgataaaaa taaggattaa gtgtaataaa
     4621 atagaaaaca agtatgttaa ttttaagtat atgctttaaa atttatccaa gttaaaaatt
     4681 taagatatct tttcagtata atggaaaaaa tgtcaaaata ttgaaatagg gaaaaaatag
     4741 tgatataata agaaaaaata gagaaaaaaa gtaaccctta gttataaaaa agttactatt
     4801 tattgaaaat ttagatattt ttctaaaaat tatctatgtt aatagttaaa tttatgaaca
     4861 ttttttgt
//
LOCUS       DAYAPA010000191         4738 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.196, whole genome shotgun sequence.
ACCESSION   DAYAPA010000191 DAYAPA010000000
VERSION     DAYAPA010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4738
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.196"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(217..1467)
                     /locus_tag="ACGI59_003625"
     CDS             complement(217..1467)
                     /locus_tag="ACGI59_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HFG9741374.1"
                     /translation="MATIKPFKAIRPNKYIVDKVAALPYDVMNSKEARRIAEGNPYSF
                     LHIDKSEIDLDENIDLYDEKVYLKARENLDEFRKQEILVKDDKECIYIYKQIMDGRAQ
                     VGIVACISVDESLNGTIKKHEYTRPEKEIDRTKHIKYCDANTGTILVTYKHQRVIDDI
                     INDFMDNNEPLYDFITDDKIEHTVWKIDNDDIIDNLVDKFEKLDYLYIADGHHRTASA
                     ENVAKEMRAKNPNYTGKEEFNYFIAMIAPDENLMVLDYNRVIKDLNGLSEEEFINKIK
                     ENFELEEIEGKEKYKPDKKGTFGMYLGDKWYKMKANKNLLEIEDPVDSLDISILQDYV
                     IDAILGIDNPRVDKRIDFIGGIRGLEELEKRVNEDMKVAFAMYPTSIEDLIRVADANK
                     IMPAKSTWFEPKVRCGLFLHEINE"
     gene            complement(1499..2440)
                     /locus_tag="ACGI59_003626"
     CDS             complement(1499..2440)
                     /locus_tag="ACGI59_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HFG9741375.1"
                     /translation="MYNILVTDGIEKEAARKLRELDFNVIEQFYEKDVLGDKLKDVDV
                     LVVRSATKVTKDVIDKALEGKKLKLIVRGGVGLDNIDVKYAQANGIKVMNTPNASSIS
                     VAELTIGQLFVLARFINTANVTMRDGKWEKKKYKGTEINGKTLGLIGFGRIAKEVAKR
                     AELLGMNVIYTDIMGEAQGFNNYKFCDMEEVLENADFLSLHIPFDKNKGAVITEKEIN
                     KMKKGAYLINCARGGLVDEKDLLKALDEGKLSAAAIDVYEQEPTLNLDLVNHPRVSPT
                     PHIGASTVEAQERIGDEIVNVIQDFFLDFNNLIGVAL"
     gene            complement(2680..3756)
                     /locus_tag="ACGI59_003627"
     CDS             complement(2680..3756)
                     /locus_tag="ACGI59_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HFG9741376.1"
                     /translation="MSKKLFIPGPIDVKEEVLQKMATPMIGHRGKDASMLQKSISEKM
                     QKLFYTNNTILLSTSSGTGLMEGSIRSCTSKKAAVFSCGSFGDRWYKMAVANNVPADI
                     FKVELGEATTPEMVDKVLSTGEYDLITVTHNETSTGIRNPIEEIGEVVKKYEDVIYCV
                     DTVSSAGGIKVEVDKIGIDICITSVQKALGLPPGMSICTFSQKAIDRAKQVPFRGVYL
                     DLLAMYEYLIKKNYQYPSTPSLSHMFALDFQLDNILDEGLDNRFNRHEDMANLVRNWA
                     KKHFQIFTNENHLSNTLTVIENTQGISVSNLNSKLQERGFQIANGYGDLKEKTFRISH
                     MGDYTVEDVQELLDNIDDILGFNK"
     gene            complement(4129..4644)
                     /locus_tag="ACGI59_003628"
     CDS             complement(4129..4644)
                     /locus_tag="ACGI59_003628"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HFG9741377.1"
                     /translation="MDFLAKYKVLKKQNSSKSCLVCGTQNELGLKADFYELENGELVS
                     ICNTKDWHQSYPGRVHGGMSAAILDETIGRAVSINDDQIWGVTVSLELKYKKPVPTDA
                     TIKVVGRITKENRKLFEGTGEIILPNGDIAVTATGKYMKMPIGQIAEGDFSNEEWFFE
                     ESKEKVEYIEL"
BASE COUNT         1435 a          866 c          485 g         1952 t
ORIGIN      
        1 ttaaaacctt ataaatatac aaatacaatt atatgaattt tcatcctcaa ttatagcata
       61 gaatatgact atctatatcc taaaattatc catttctaaa aaaaaggtaa ctattattaa
      121 gaatatcaac ttatagaatt tatattccac atacgatttt caatcaaaaa aacaagggct
      181 aaaaagtgaa ttgtgcactt tttagccctt tattaattat tcatttattt catgtaagaa
      241 tagaccacat cttactttag gttcaaacca tgttgactta gctggcatta tcttatttgc
      301 atctgctact cttattaaat cttcaatact agtaggatac attgcaaatg ctactttcat
      361 atcttcatta actctttttt ctaattcttc aagacctcta attccaccga taaaatcaat
      421 tcttttatct actctaggat tatcaattcc taaaatagca tcaataacat agtcttgtaa
      481 tatagaaatg tctaatgagt caacagggtc ttctatttct aataaatttt tatttgcttt
      541 cattttatac cacttatcac ctaggtacat tccgaaagtt ccttttttgt caggtttata
      601 tttttctttt ccttctattt cttctagttc aaaattttct tttattttat ttataaattc
      661 ttcctcgctt aaaccattta agtctttgat aaccctatta taatccaaaa ccattaaatt
      721 ctcatctggt gcaatcattg ctatgaagta attaaattct tcttttccag tataatttgg
      781 atttttagct ctcatttctt tggctacatt ttctgctgaa gctgtcctat gatgaccatc
      841 tgcaatgtat agataatcta atttttcaaa tttatctact aggttatcta ttatatcatc
      901 attatcaatc ttccatactg tatgctctat cttgtcatct gttatgaaat catataaagg
      961 ttcattatta tccataaaat cattgattat atcatctatc accctttgat gtttgtaagt
     1021 tacaagtata gttcccgtat tagcatcaca gtattttatg tgtttagttc tatcaatttc
     1081 cttttctggt cgagtgtact catgtttttt tattgttcca ttcaagcttt catcaactga
     1141 tatacatgct actataccaa cttgagctct accatccatg atttgtttat atatatatat
     1201 acactctttg tcatctttta ctagaatttc ttgttttcga aactcatcta aattttctcg
     1261 cgcttttaaa taaacttttt catcatataa atctatattt tcatctaaat caatttctga
     1321 tttatctata tgcaaaaatg aatatggatt cccttctgca attcttctag cttctttact
     1381 attcataaca tcgtaaggaa gtgctgctac tttatctact atatatttat ttggtctaat
     1441 cgccttaaat ggtttaatcg ttgccattac taatttcctc ctaattatac aaaatatttt
     1501 ataatgctac ccctattaga ttgttgaagt caagaaagaa atcttgaatc acatttacta
     1561 tttcgtctcc tattctttct tgagcttcta ctgtagatgc cccaatatga ggtgtaggag
     1621 aaactcttgg atgatttact aagtctaaat ttaatgtagg ttcttgttca taaacatcta
     1681 tagcagctgc tgataattta ccttcatcta atgcttttag taaatctttt tcatcaacta
     1741 aaccgcctct tgcacagttt attaaataag cccctttctt catcttattt atttcttttt
     1801 cagttattac tgccccctta ttcttatcaa atggtatgtg taaagataag aaatctgcat
     1861 tttctaaaac ttcttccata tcacagaatt tataattgtt aaagccttgc gcctcaccca
     1921 ttatgtcagt atatataaca ttcataccta ataattctgc tctctttgca acttcttttg
     1981 caattcttcc aaaacctata agtccaagtg tctttccatt aatttctgta cctttatact
     2041 ttttcttctc ccattttcca tctctcatag ttacatttgc agtatttatg aatcttgcaa
     2101 gaacaaataa ttgacctatt gtaagctctg caacagatat actgcttgca tttggagtat
     2161 tcataacttt tatgccatta gcttgagcat atttaacatc tatattatcc agacctactc
     2221 cacctcttac tatcaacttc aactttttac cctctaatgc tttatctatt acatctttag
     2281 taactttagt agctgaacgt actacaagta catcaacatc tttaagttta tctcctaaaa
     2341 catctttttc ataaaactgc tctatcacat taaaatctaa ctctctcaat tttctcgctg
     2401 cttctttctc tattccatca gtaactaata tattatacat aacaaactcc tccaattttt
     2461 attcacttaa tttttccaat ttttattttt taaagtattt tcatcaataa aaaactttac
     2521 ttaatttaac tttctgttta tataattttc aaaatatttt tttattaaag tcatatcaaa
     2581 tttttctatt gtttgttaaa aatttaatat gtaattttta taaaatatta tttattatat
     2641 attctaaatt tatcttaaaa catacatatt tttacaatat tatttattaa aacctaatat
     2701 atcatcaata ttatctaata attcttgtac atcttcgaca gtatagtcac ccatatgtga
     2761 aattctaaaa gttttttctt ttaaatctcc atagccattt gctatttgga atcctcgttc
     2821 ttgtaatttt gaatttaaat ttgatacact aataccttga gtattttcta taacagtaag
     2881 tgtattggat aagtgatttt cattggtaaa aatttggaag tgttttttag cccagtttct
     2941 aactaagttt gccatgtctt catgtctgtt gaatctgtta tctaggcctt catctaatat
     3001 attatctaat tggaaatcta aagcgaacat gtgagaaaga gatggtgttg aagggtattg
     3061 gtagtttttc ttgattaagt attcatacat tgctaacaaa tcaagatata ctcctctaaa
     3121 aggtacttgc ttagctctat cgattgcttt ttgagaaaat gtgcaaatag acattcctgg
     3181 tggtaatccc agtgcttttt gtactgatgt tatacaaata tctattccaa tcttatcaac
     3241 ctcgactttt attccaccgg ctgaacttac agtatctaca cagtagatta catcctcata
     3301 ctttttaaca acttctccaa tttcttctat tggatttctt attccagttg aagtttcatt
     3361 gtgagttaca gttattaaat cgtattctcc ggtagaaagc accttatcaa ccatttctgg
     3421 cgttgttgcc tctcctaatt caactttaaa tatatccgct gggacattat tagccactgc
     3481 cattttgtac catctatcac caaaggatcc acaggaaaat actgctgcct ttttggatgt
     3541 acaggaacga atggagcctt ccattaaacc agttccggat gacgtggata aaagtatcgt
     3601 gttgtttgta tagaatagtt tttgcatctt ttcacttata ctcttttgga gcatagatgc
     3661 atcttttcct ctatgtccta tcattggtgt agccatcttt tgcaagactt cttctttaac
     3721 atcaatagga ccaggaataa ataatttctt actcattata attcccccct ttttagtttg
     3781 taatatggat agcgttttta ctgtccttat ttttaataac tataatatca agctgaatta
     3841 actatgtcaa cataaaattt attttttata tataatttat tttatatatt ttatgaaaac
     3901 attcattttc taccttttca taagtctgaa ttttctgaat tattccctca aaaaaaatat
     3961 actttttatc tatataaatt aattttttta gtatgtaaat tttacgctat tacgataaat
     4021 ctttttagta taactaattt atttttatct tgtattttta ttgtattttt gaattttatt
     4081 ttttctaaat ataaaattta tgttttaaaa tataaaattt ttatttaatc aaagttcaat
     4141 atattccact ttttctttcg attcttcaaa aaaccattct tcattagaaa aatcaccttc
     4201 tgcaatttgt ccaataggca ttttcatata ctttccagtt gctgtcactg ctatatctcc
     4261 atttggtaga attatttctc ctgtaccttc aaataatttt ctgttttctt tagttattct
     4321 tccaacaact tttatagtag catctgttgg tactggtttc ttatatttca actctaaaga
     4381 tactgttact ccccatattt ggtcatcatt tatacttaca gctcttccaa tagtctcatc
     4441 caatattgca gcagacatac ctccatgaac tctcccagga taactttgat gccaatcctt
     4501 tgtattacaa attgatacaa gctctccatt ttcaagttca taaaaatctg ctttaagtcc
     4561 tagctcattt tgtgttccac aaactaaaca agatttacta ctattttgct tcttcaaaac
     4621 tttatacttt gccaaaaaat ccatctccta accgacatta gtagtcgctt aatattaaat
     4681 aattctaaaa tatattatat aatattaata aaatccctgc aaacttctaa taataaaa
//
LOCUS       DAYAPA010000192         4644 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.197, whole genome shotgun sequence.
ACCESSION   DAYAPA010000192 DAYAPA010000000
VERSION     DAYAPA010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4644
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.197"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     misc_binding    433..671
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            735..2654
                     /gene="thrS"
                     /locus_tag="ACGI59_003629"
     CDS             735..2654
                     /gene="thrS"
                     /locus_tag="ACGI59_003629"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFG9741378.1"
                     /translation="MIKVALKDGSIKEFENAISVMDVAKSISEGLARNVVAASVNGEV
                     VGLDHIIDTDCDLNLFKFEDKEGKEVFRHTSAHILAQAIKRLYPEAKLAIGPSIENGF
                     YYDIDLDHRLVPEDLEKIEAEMKKIAKEDLKIERFELPRNEALELMKEQGEDYKVELI
                     SDLPESEIISFYKQGDFTDLCRGPHLPSTKKVKAVKLQSVAGAYWRGDENNKMLQRIY
                     GTSFEKNKDLEEYLHLLEEAKKRDHRKLGKELGLFMIPEEGPGFPMFLPKGMELKNEL
                     LKFWREIHRKAGYIEIESPIILNRKLWETSGHWYHYKENMYTVKIDDEDYAIKPMNCP
                     GGLIYYNSQLHSYRDFPMRVAELGRVHRHELSGALQGLMRVRAFTQDDSHIFMLPEQI
                     KDEIKGVANLIDGIYKTFGFEYNLELSTRPENSMGSDEEWEAAENGLREALEELGLPY
                     TINEGDGAFYGPKIDFHLKDCLGRTWQCGTIQLDMQLPRQFDNTYIGQDGEKHRPVMI
                     HRVAFGSIERFIGILIEHYAGKFPVWLSPTQVKILPISDKFMDYANEVKKELFDKGIR
                     VELDDRAEKIGFKIREAQLEKVPYMLIVGEKEVADNNVSVRSRDKGEIGSIKLDEFIA
                     SISKEIESRESIIQD"
     gene            3422..4513
                     /locus_tag="ACGI59_003630"
     CDS             3422..4513
                     /locus_tag="ACGI59_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFG9741379.1"
                     /translation="MIEKVEEKSKIIKTSKEYKRTIIEEKDKEWTKKLNDILSRSSFS
                     NDELDKIKALIPKEILTNENVGEVVTEDGYAKPEYDEVFKSLFTLNNEYDLLVNFIND
                     ILKDAPYANRNIKPFTQIKRIIRVETDPTINYIGEKRPKLDILAEDEENNHINIEMQR
                     AFENDYLERAEYYLSRVHGRKLEEGKEYKEIGKTIGIHILNHVKYNHIEDYVNCLRLT
                     MDGHPDIYSSKTALYFIELPKIHKSDYIVNRIMLWGKLISNPSNLDVRVIAKNDSIIK
                     KALDRLKELGSNEEYLLNLKRGAYIMNKSRNFKEEIERETAIRVAKEKDYKIIIMLKK
                     NGFKLDDILNKFNDFDLSEDEIKEVYENN"
BASE COUNT         1839 a          455 c          834 g         1516 t
ORIGIN      
        1 atattaattg tatttaccta tttgagtgaa ttacttatta atagcattta gatattaatt
       61 gtatttattt atttgattgt gttacttatt aatagtattt aagtatttac tgtactactt
      121 attaaccata ttaagtattg attgtattta agtatacaaa acctaaaaag agtacaaaaa
      181 ttagaagtta gttttaataa aaattagttt tagtatttat ctagtcatat ctatcatgca
      241 tgtggatacg ttttaaaaat tttaaattta tgaaaatatt ataatataac tataaatcaa
      301 gagattaatt taatataaaa atataagaat attggtttac agctgattga gatgaaaaat
      361 aatgggtaat taatatatta cattaatgtt gattgatgta taactatctg ttataatgaa
      421 aatatattaa aaatcatagt gttgaagagg aagagtagag tagaaaccat tctcagagaa
      481 tttgggtcta tggctgagag cccatgtaat aggtctattt gaaaactacc ttggagctga
      541 gcttattagc agtatttgta tttatactat acgtgaaagt gctcgggtga cgaccttata
      601 gtcatcaaga gggttgtata tacttgtata cgccaacttg ggtggaaccg cgaattaata
      661 tcgtcccaaa catatttggg gcgatttttt attacaaaaa atctaaaaat tatttcaata
      721 aggaggaaaa aacaatgatt aaagttgctt taaaagatgg ctcaataaaa gaatttgaaa
      781 atgctatttc tgttatggat gtagctaaat ccattagtga aggattagct agaaatgtgg
      841 tagcagcgtc tgtgaacgga gaggttgtag gtctggacca cattatagat actgattgtg
      901 acttgaattt atttaagttt gaagataaag aaggtaagga agtttttaga catacatcag
      961 cgcatatatt agctcaagct ataaaaagac tttacccaga agcaaagctt gctataggac
     1021 caagtataga aaatggattc tactatgata tagatttaga ccatagatta gttccagagg
     1081 acctagagaa aatagaagct gaaatgaaaa aaatagctaa agaagattta aaaatagaaa
     1141 gatttgaact tcctagaaat gaagctttag agcttatgaa agaacaagga gaagactata
     1201 aggtagaact tatttctgat ttacctgaaa gtgaaataat atctttctac aagcaaggtg
     1261 attttactga tttatgtaga ggaccacatt taccatctac taaaaaggta aaagctgtta
     1321 aattacaaag tgtagcaggt gcatattggc gtggtgacga aaataataag atgcttcaaa
     1381 gaatatatgg aacttctttt gagaaaaaca aagacttaga agagtactta catttactag
     1441 aagaagctaa aaagagagac cacagaaaat taggtaaaga attaggatta tttatgatac
     1501 ctgaagaagg tccaggattc cctatgtttt taccaaaagg tatggaactt aaaaatgaat
     1561 tattaaaatt ctggagagaa atacacagaa aagctggata tattgagata gaatctccaa
     1621 tcatattaaa tagaaaatta tgggagactt caggacactg gtatcattac aaagaaaata
     1681 tgtatacagt taaaatagat gatgaggatt atgctataaa acctatgaac tgtcctggtg
     1741 gattaattta ctataactct caactacatt catatagaga tttcccaatg agagttgcag
     1801 agcttggtag agttcataga catgagttat ctggtgcatt acaaggatta atgagagtta
     1861 gagcatttac tcaagacgat tcacatatat tcatgttacc agagcaaatt aaggacgaaa
     1921 taaaaggagt agctaactta atagatggta tatacaaaac ttttggattt gaatataatt
     1981 tagaattatc tacaagacca gagaactcta tgggaagcga cgaagagtgg gaagcagcag
     2041 aaaatggatt aagagaagct ttagaagagt taggtctacc atatactatt aatgagggtg
     2101 atggagcatt ctatggtcct aagatagact tccatctaaa agattgttta ggaagaactt
     2161 ggcaatgtgg tactatccaa ttagatatgc agcttccaag acaatttgat aatacttata
     2221 ttggtcaaga tggtgaaaag catagaccag ttatgattca tagggttgct tttggtagta
     2281 tagaaagatt tataggtata ttaatagagc attatgctgg taaattccca gtttggttat
     2341 ctccaactca agttaagata cttcctatat ctgataaatt tatggattat gcaaatgaag
     2401 ttaagaaaga gttatttgat aagggtataa gagttgaatt agatgataga gctgaaaaaa
     2461 ttggatttaa gataagagaa gctcagcttg aaaaagtgcc atacatgtta atagtaggtg
     2521 aaaaagaagt agctgataat aatgtttctg ttagatcaag agataaaggt gaaataggta
     2581 gtattaaatt agatgaattt atagctagta tttcaaaaga aattgaatca agagaaagta
     2641 ttattcaaga ctaatatata ttatattagt agctaagata ttgttatata agataagttt
     2701 aatcaatatg ttgatatgtt gatgcatagt gattaaattt atgaaaagat gagtataatt
     2761 agaggtacag ttaatcatat ttttatgatt aatgtacctc tagttttttg tatggtagat
     2821 ttattaataa atatatcata gaagtgtttt gaacatggaa atatttatgt gcattcttta
     2881 aataaaagtt tattttttga gtgataatta agtgcttgtt ggtaaggtta tggctgtaaa
     2941 aatcataact ttattttcat atttgttcca ttttcataaa aattattatc cctttaagaa
     3001 aatttcttaa tcctcttgaa atatttcctt tttagtctaa agttaaattt ctttcttcaa
     3061 aatgaatatt taaatgaact aaaatttaag tacattttct aatcttatga gtctattttt
     3121 catagataat tgccctataa tagaagaatc tatgttggtg ctatgtggtg acaataacag
     3181 cattgtcagt gattaattaa tgctttataa atgattaatt agtgttttat gttatttttg
     3241 gacttatcct ctatacatag attaatttat ttaactttag atagagagag aatgtcaagt
     3301 acatacacag acattagagt ttaagaaagt gtttatggag caagaacttt taaaagaggt
     3361 agtgacttag tttaatttca agaaatgata aatacaaaag aggaaaaaga gaggtgttga
     3421 aatgatagaa aaagtagaag aaaaatctaa gattattaaa acatcaaaag aatataagcg
     3481 aactataatt gaagaaaaag ataaagaatg gaccaaaaaa ttaaatgata tacttagtag
     3541 aagtagtttt tctaatgatg aattagataa aatcaaggca ttaattccaa aagagatttt
     3601 gactaatgaa aatgttggag aagttgtgac tgaagatgga tatgctaagc ctgagtatga
     3661 tgaggtattt aaatcattgt tcactttaaa taatgaatat gatttacttg taaattttat
     3721 aaatgatatt ctaaaagatg ctccatatgc taacaggaat atcaaaccgt tcactcaaat
     3781 aaagagaata attagagttg aaactgatcc aacaattaat tacattggag agaaaagacc
     3841 taaacttgat attttggctg aagatgaaga aaataatcat ataaatattg agatgcaaag
     3901 agcttttgag aacgattatt tggaaagagc tgaatattat cttagtcgtg tacatggtag
     3961 aaagttagaa gaaggtaaag agtataaaga aattggaaaa actattggaa tacatatact
     4021 aaatcatgta aaatataatc acatagaaga ttatgtaaat tgcctgagat taacaatgga
     4081 cgggcatcca gatatttatt ctagtaaaac tgcattatat tttatagagc ttccaaaaat
     4141 tcataaatct gactatatag taaatagaat tatgctatgg ggaaaactta ttagtaatcc
     4201 ttctaactta gatgtaagag ttatagctaa gaatgattct attattaaaa aagcacttga
     4261 tagattaaaa gaattaggta gcaatgaaga atatttatta aatctgaaaa gaggtgctta
     4321 tatcatgaat aaaagtagaa attttaaaga agagattgaa agagagacag caattagagt
     4381 tgcaaaagaa aaagattata aaataattat aatgttgaaa aaaaatggtt ttaaattaga
     4441 tgacatatta aataaattta atgattttga cttaagtgaa gatgagataa aagaagtata
     4501 tgaaaataat tgatgataaa taaaaagtga acttatataa gtttgctgaa tcggagtctg
     4561 gaaatattta tatgtatttt ttaatgaatg aaaatttatt ttaaaaacaa taatttagaa
     4621 gtacagttaa ttatattttt atga
//
LOCUS       DAYAPA010000193         4620 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.198, whole genome shotgun sequence.
ACCESSION   DAYAPA010000193 DAYAPA010000000
VERSION     DAYAPA010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4620
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.198"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            561..1868
                     /locus_tag="ACGI59_003631"
     CDS             561..1868
                     /locus_tag="ACGI59_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9741380.1"
                     /translation="MKEIADAITSEYLSYGINEDISIYEGRFCIYLDKKYRCNGKIYY
                     KMTPPISISFKADIGCVEEIDNEDDNLALDYDNAILEVHGYKIISITINTLSEFSVEG
                     YINDDCIKSKNSYVEYVDFNIINLDKIPGKLIKYNDKVYAGRIEFDINDYVVTIDKRY
                     DYRKELKSELKSKSGAIITHIGRIRRKDGRIFRTNNTINLLDRISTALSFMCGRYVGF
                     CLAKGYRSGNEVYRIWNENQISPFRYVPTWSDTLSNYHNMEKYISLMCKKLEDFYYGS
                     AIKSVVDWYIESLGSATMENNIISVQIALETLSYVILVEQNKILTDEAFDCNLASKNI
                     RLLLDTCKIPYGKHELNIFDNIIKNKFDDGVDLVIYLRNSIVHPSRKTHRAVLEVEDI
                     WNIISIGTRYIELVLLFILGYRGEYSNRLVERCYGEVEVVPWN"
     misc_binding    1954..2216
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2342..3145
                     /locus_tag="ACGI59_003632"
     CDS             2342..3145
                     /locus_tag="ACGI59_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFG9741381.1"
                     /translation="MKNILKKVGIFTIMLGLLGGVVGCSKPDNEKDKDASKESKKEVV
                     VGFDNTFVPMGFLDEKGDTVGFDVDLAKETFKRLGMEVKFQPIDWSMKETELNDSKTV
                     DVLWNGYSITDERKKIVSYTEPYLQNKQIIVTLSDSKINSKADLKDKEVGTQQGSTAL
                     DAVEKDKDFMNSLKGGAPVLYDTYDKALRDLEIGRTSAVVGDEVLIRYYMGQKGEDKY
                     KVLKDDFGLEDYVVATSKENPELCEKINETLKEMKKDGTFDKIYDKWFK"
     gene            3183..3794
                     /locus_tag="ACGI59_003633"
     CDS             3183..3794
                     /locus_tag="ACGI59_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFG9741382.1"
                     /translation="MLQGLEIVIAMFCITLIVSIPLGIGVAFLRLSKNKLVSGITQCY
                     ILIMRGTPLLLQMIVIFYGLPLLGIVFDRFTAGVVAFFLNYAAYFAEIFRGGIQSIDR
                     GQYEASKVLGFDKFTMYKRVIFPQVFKRILAPISNEVITLVKDTSLVYILGLNDILRI
                     SQIAMNREASLLPLFEAGAIYLIFVAILTKGFELLEKKYSYYR"
     gene            3826..4497
                     /locus_tag="ACGI59_003634"
     CDS             3826..4497
                     /locus_tag="ACGI59_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFG9741383.1"
                     /translation="MLKIKNLNKSFKKNRVLKDISFELEEGQIGVLLGKSGAGKTTIL
                     RCINGLEEFDSGEIIIDNEVIKNKRDMAKIRGKIGMVFQNFNLFPHMTVLENIIESPV
                     NVFKVPRKEAEERARELLRLVDLEDKLNSYPFELSGGQQQRVAIARSCALMPKVLCFD
                     EPTSALDIDTIQRVVNIMNRLKDKGMTILIITHDVVFSNNVADKIISIKDGIVENVQI
                     KEKIV"
BASE COUNT         1853 a          424 c          827 g         1516 t
ORIGIN      
        1 ataaattaat tatgaaaaat ttatataagt aaaatatatt aacataaagg taaaattttg
       61 tatactaaaa gggaatattt acagtttaat ggcagtttta aaataataaa ttaaagctgt
      121 catttttatt taaaaatttt tagaaaaagt acaatagtgt taatgtgtga attagataag
      181 gtgtatataa tagtttagat gatatgatat aaaatatgtt gtaaattaaa tgttttcttt
      241 gacaatatat acacaaggat gataatatct atatgaataa aagatagtgt gtaatttgat
      301 aatatgtaat tataacatta tgatataatt acaataaagt atgtaataat ctgtagaaat
      361 ttcaagctag ttatctaagt tttcggataa ctaaatctaa acggaataca taatgtatta
      421 aataaatgat gatactaaaa tgatactatt tttatagaaa tttattgagg tatataatta
      481 atgaaaataa tatattaaat tatataaatt aaattatata atatattgag ttttacataa
      541 aagcaaaggt gggaataaaa atgaaagaaa ttgctgatgc tataacatca gagtatttaa
      601 gctatggtat caatgaagat attagtatct atgaaggtag attttgcata tatttagata
      661 aaaaatatag atgtaatggg aaaatttatt ataaaatgac acctccaata tctataagtt
      721 ttaaagcaga cataggttgt gttgaagaga tagacaatga agatgacaat ttagctttag
      781 attatgataa tgctattctt gaagttcatg gatataaaat tatttctata acaataaata
      841 ctttaagtga atttagtgta gaaggttata tcaatgatga ctgtataaaa tctaagaact
      901 cctatgttga atatgtagat tttaatataa taaacttaga taaaatacct ggaaaactca
      961 ttaaatacaa tgataaggta tacgcaggta gaattgaatt tgatataaat gattatgttg
     1021 taactataga caagcgatat gattatagaa aagaattaaa atcagaatta aagtctaaat
     1081 ctggagcaat aataactcat ataggcagga ttagaagaaa agatggtaga atttttagaa
     1141 caaataatac aataaatctt ttagatagga tatcaacagc tttgagtttt atgtgtggaa
     1201 gatatgtagg gttttgtttg gccaaagggt atagaagtgg taatgaagtt tatagaatat
     1261 ggaatgaaaa tcaaatatct ccttttagat atgtaccaac atggtcagat acactttcaa
     1321 attatcataa tatggaaaaa tatataagcc ttatgtgtaa aaagctagaa gatttttatt
     1381 atggttctgc tataaagagt gttgttgatt ggtacataga atctcttgga agtgctacca
     1441 tggaaaacaa tataatatct gtacaaattg cacttgaaac tttgtcatat gtaattctag
     1501 tggaacaaaa taagatactt acagatgaag catttgactg taatttggca tctaaaaata
     1561 taagactctt acttgataca tgtaagatac catatggtaa acatgaatta aatatatttg
     1621 ataatataat aaaaaataaa tttgatgatg gagtggattt agttatttat ttaagaaata
     1681 gcatagttca tccaagtaga aagactcata gagctgtgtt agaggttgaa gatatttgga
     1741 atataatttc aataggaacg agatatattg aacttgtttt actctttatt ttaggttata
     1801 gaggagaata ttcaaacaga ttagtagaaa gatgttatgg tgaagtggaa gttgtacctt
     1861 ggaactagat ttttatattt attttaaaat atatattgac atatattgcc ttatgctata
     1921 aaatcaatat cataataata attcagaata acattaaatg ctaagaaaag aaatagtaaa
     1981 agtagttaat cttaaacaga gagtttccga ttggtgagag gagcaataga ttttactttg
     2041 aacacatctt tgagcaggac tctgaaagag tagaaatact ttattaggag atttccggaa
     2101 tccttgcacc cgttatagtg ctagagtata ataattttat tgtacttaaa gaggttagtg
     2161 ccgtgaggca ttaatgaaca gaggtggaac cacgagagtt aactcccgtc ctctagcaga
     2221 agctagaggc gggagttttt taatataatt ttttaagcgc gcagagaatt atattgcaat
     2281 agatacactt tttaaaaaat aatcaaaata ttttaaaaat taaaagacga gggagaatat
     2341 tatgaaaaat atattaaaaa aagtaggaat atttacaatt atgttaggtt tattaggtgg
     2401 agttgtaggt tgttcaaaac cagataatga aaaagataag gatgcttcaa aggaatctaa
     2461 aaaagaagta gttgtagggt ttgataatac gtttgtacca atgggatttt tagatgaaaa
     2521 gggagataca gtaggctttg acgtagactt agctaaagaa acatttaaaa gacttggaat
     2581 ggaagttaag tttcagccta tagattggtc aatgaaggaa actgaattaa atgattcaaa
     2641 aactgtagat gtattatgga atggatattc tataacagac gaaagaaaga aaatagtatc
     2701 atatacagaa ccatatttac aaaataaaca gataatagtt actctaagtg actcaaaaat
     2761 aaattcaaaa gctgatttaa aagataaaga agttggaaca caacaaggtt caacagcact
     2821 tgatgcagtg gaaaaggata aagattttat gaatagctta aaaggtggag caccagttct
     2881 ttatgatact tatgataaag cacttagaga tttagagata ggtagaacta gtgcagttgt
     2941 tggtgatgaa gttttaatta gatactatat gggacaaaag ggtgaagaca aatacaaggt
     3001 attaaaagac gattttggat tagaagatta tgttgtagca acttctaaag aaaatccaga
     3061 actttgtgag aaaataaatg aaacattaaa agaaatgaaa aaagatggaa cttttgacaa
     3121 gatttatgat aagtggttta aatagaagat aagttagctg ttaagaaggg ggcactaaag
     3181 gcatgttaca aggtttagag atagttatag caatgttttg tataacattg atagtatcaa
     3241 ttccattggg gattggagtt gcatttttaa gattatcaaa gaataaatta gtaagtggta
     3301 ttacacaatg ttatatattg ataatgagag gtacaccttt acttttgcaa atgatagtca
     3361 tattttacgg attacctctt ttaggaattg tatttgacag atttacagct ggagtagtag
     3421 ctttcttctt aaattatgca gcatattttg cagaaatatt tagaggtgga atacagtcaa
     3481 tcgatagagg acaatatgaa gcttcaaaag tactaggatt tgataagttt actatgtata
     3541 agagagtaat ctttccacaa gtttttaaaa gaatactagc acctatttca aatgaagtaa
     3601 tcacattagt aaaagataca tctttagtat atatattagg attaaatgat attttaagaa
     3661 tatctcaaat agcaatgaac agggaagcaa gtctgttacc attatttgag gcaggagcga
     3721 tatatcttat atttgtagct atacttacta agggatttga attacttgaa aaaaaatatt
     3781 cttattatag ataaagttac aatattatag gaggaggttt tttatatgtt gaaaatcaaa
     3841 aacttaaata aatcatttaa aaaaaataga gtacttaaag atatttcttt tgagttagaa
     3901 gaaggtcaga ttggagtttt acttggtaag tctggtgcag gaaagaccac aatattaagg
     3961 tgtataaatg gtcttgaaga atttgatagt ggagagatta taatagataa tgaggtaata
     4021 aaaaataaga gagatatggc aaaaatacga ggaaaaatag gtatggtttt tcaaaatttc
     4081 aacttatttc ctcatatgac agtccttgaa aatataattg aatccccagt taatgttttt
     4141 aaagttccta gaaaagaggc tgaagagagg gctagagaac ttttaagatt agttgattta
     4201 gaagataaat taaattctta tccatttgaa ttgtcaggag gacagcaaca gagagttgct
     4261 atagctaggt catgtgcatt gatgccaaaa gtcctttgtt ttgatgaacc tacttctgca
     4321 cttgatattg atactattca aagagtagtc aatataatga acagacttaa agacaaaggt
     4381 atgacaatat taataattac acatgatgtt gtcttctcaa ataatgttgc agataaaatt
     4441 ataagtataa aagatggtat agtagaaaat gttcaaataa aagaaaaaat tgtttagagt
     4501 tttataaata taaactaggg tagaatttta taatataaat aaagggcaag taaaatcaaa
     4561 tatactatgc ccttttgtta tctcaaaatt taatcttata ttttatgttt aagttacatt
//
LOCUS       DAYAPA010000194         4614 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.199, whole genome shotgun sequence.
ACCESSION   DAYAPA010000194 DAYAPA010000000
VERSION     DAYAPA010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4614
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.199"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     misc_binding    238..472
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            522..1541
                     /gene="pheS"
                     /locus_tag="ACGI59_003635"
     CDS             522..1541
                     /gene="pheS"
                     /locus_tag="ACGI59_003635"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFG9741384.1"
                     /translation="MQEKLLALREAALAEIKEAQSIESVESLRVKYLGKKGEITAILK
                     EMGKLSAEERPVVGKVANEVRENIELSINSKKEEINAIEKERKLKEEVIDVTQPGKVL
                     KVGKKHPITQIIDEVTDIFIGMGFSIAEGPEVETVENNFDALNAPKDHPSRDMSDTFY
                     INDGVLLRTQTSPVQVRTMRSQELPIKVIAPGRCFRSDSPDATHSPMFHQIEGLVVGK
                     DVTMAEFKGTMDIFVEKLFGSDIKTKFRPHNFPFTEPSAEVDVTCFKCGGKGCPMCKY
                     EGWIEILGSGMVHPNVLRNCGIDPEVYSGFAFGVGVERLAMLKYEIDDIRLLFENDMR
                     FLNQF"
     gene            1558..3951
                     /gene="pheT"
                     /locus_tag="ACGI59_003636"
     CDS             1558..3951
                     /gene="pheT"
                     /locus_tag="ACGI59_003636"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFG9741385.1"
                     /translation="MLVSLKWLRDYVDIDMDVKEFADKMTMTGTKVETIDYYGEEIEN
                     ILVGKILEIKQHPNADKLVVTKVDIGDKVVQIVTGATNISEGDYIPVAVNGSKLPGGV
                     EIKQTDFRGELSDGMMCSAAELGIDEHYIEEYKRGGIYILDHEDSYELGKDIKDVLGL
                     KDALIDFELTSNRPDCKCMMGIAREAAATIGTKVKYPEIEVKESDEEIDFKVEIDNPD
                     LCRRYVARMVTDVKIEPSPYWMQRRLTEAGVRPISNIVDITNFVMLELGQPLHAFDIN
                     QVETGRIVVRNAKDGEKLVTLDDVERTLDKDMLVITNGEKSLGLAGVMGGANSEITSN
                     TKTVLFESANFKPENIRMTAKKVGIRSEASSRNEKDLDPNLAEIAANRAAQLVEMLGA
                     GKVLKGVVDVYPNKPEPKKLVVNPQRINHLLGVDVPMEQFVGILESLEFKCNLVANDK
                     LEIDVPSFRTDMEQEADVWEEIARIYGFENIPSVQLEGNTTAGIKTSKQKFMDALKDN
                     STAVGLNEILTYSFVSPKGVDKIRVPEGNAKRNFVKLLNPLGEETSVMRTTLIPNMLD
                     VLSTNVSHKIEEVSAFECGHIFIPQDSELPKEENRMCVGMYGKDVDFFALKGTIETIL
                     VNVGFKGYEIEPQDNNTTFHPGRCAKIVYNNKYVGTLGELHPDVIENYNLGQRVYVAE
                     IDIDFVFDNSDRTKNYVPLPKYPSTSRDIALIVKDDVFVKQIEDIIKENGQGLVESYK
                     LFDVYKGSQIEAGYKSIAYSITYRSKDKTLTDEDVAKVHDKILSELSEKLNANLRSN"
     gene            3976..4566
                     /gene="zapA"
                     /locus_tag="ACGI59_003637"
     CDS             3976..4566
                     /gene="zapA"
                     /locus_tag="ACGI59_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFG9741386.1"
                     /translation="MNKVMVKIHGAEYPMVGDKSEKFMISIADFVDKEMDKITRQNPK
                     LSLSVAAILTALNISDLLFECSDENEKLIKANEELSKKVGASNEELQLEIKSLKLTIA
                     EKEAENREAETKMKELIEIIENKKQEIFELSNTTEGSRAELDAYKNKIEELSTQLEEA
                     NERATIAENLASEFQNKAYDLQLKCTGLNNDAKNVE"
BASE COUNT         1829 a          523 c          934 g         1328 t
ORIGIN      
        1 atgtatatta tataaaaaat aattgagtta attatgcatt agatgtatat tatataaaaa
       61 ataattgagt taattatgca ttaggtgtat attatataaa aaataattga gttaattatg
      121 cattagatat atattatata aaaaataatt aaattaatta tgtattggat atatattaaa
      181 aatagtcaca taatttgaat ggaaatgata taatactaaa ataaacaata taatattgta
      241 aatgcaatga aagaggaaag tattttgatt aaacttagta aagagataaa cacctaggct
      301 gggagtgttt tctaagaggt catgagaagt tccctctgga gtaacagagc tgaaatttta
      361 cagtaggctt tgacgtcaaa aacgcgttaa gtttgttaga ggtggtttga tgatttttaa
      421 attgttaaac tactagggtg gtaccgcgaa actatagcct ttcgtcccta gacagggatt
      481 gaggggcttt ttttatacaa aaaaacgaaa gggtgatatg tgtgcaagaa aaattacttg
      541 ctttacgtga agcagctttg gctgaaataa aagaagcaca aagcatagaa agtgtagaaa
      601 gtttaagagt taagtactta ggaaaaaaag gtgagataac tgccatactt aaagaaatgg
      661 gtaaattatc tgctgaagaa agaccagtag ttggtaaggt tgccaatgag gtaagagaaa
      721 acattgaact tagcataaat tctaaaaaag aagaaataaa tgctattgaa aaagaaagaa
      781 aattaaaaga ggaagtgata gatgttactc aaccaggaaa agttttaaag gttgggaaga
      841 agcatccaat aactcaaatt atagatgaag taacagatat atttatcgga atgggattct
      901 ctatagcaga agggccagaa gttgagactg ttgaaaacaa ctttgacgca ttaaacgctc
      961 ctaaagacca tccatcaaga gatatgagcg atacattcta tatcaatgat ggggtattac
     1021 ttagaactca aacatctcca gttcaagtaa gaactatgag aagtcaagag ttaccaataa
     1081 aagtaattgc accaggtaga tgttttaggt cagactcgcc agatgctaca cactcaccaa
     1141 tgttccatca gatagaaggg cttgttgttg gaaaagatgt tactatggca gaatttaaag
     1201 gaactatgga tatcttcgtt gaaaaattgt ttggttctga tatcaaaact aagtttagac
     1261 ctcacaactt cccatttaca gaaccaagtg cagaggttga tgttacttgt ttcaaatgtg
     1321 gtggtaaagg ttgcccaatg tgtaaatatg aaggttggat agaaatatta ggttcaggta
     1381 tggttcatcc aaatgtgctt agaaattgtg gaatagaccc agaagtttac agtggatttg
     1441 catttggagt tggggttgaa agacttgcaa tgcttaaata cgaaatagat gatattagat
     1501 tattattcga aaatgatatg agattcttaa atcaatttta attaggaggg tatgtagatg
     1561 ttagtatctt taaaatggct tagagactat gttgatatag acatggatgt aaaagagttc
     1621 gctgataaaa tgacaatgac aggaactaaa gttgaaacaa tagattatta tggtgaagaa
     1681 atagaaaata tattggttgg aaagatttta gaaataaaac aacatccaaa tgctgataag
     1741 ttggttgtaa ctaaagtaga tattggagat aaagttgttc aaatagttac aggagctaca
     1801 aatatatcag aaggagatta tattccagta gctgtaaatg gttctaagtt acctggagga
     1861 gttgaaatca aacagactga tttcagaggt gaattatcag atggtatgat gtgttcagca
     1921 gctgaactag gtatagatga acattacatt gaggagtata aaagaggtgg tatatatatt
     1981 ttagaccacg aagattctta tgaattagga aaagatataa aagatgtttt aggattaaaa
     2041 gatgctttaa tagattttga attaacttca aacagacctg attgtaaatg catgatgggt
     2101 atagctagag aagcagctgc aactatagga acaaaagtaa aatatcctga aatcgaagta
     2161 aaagaaagtg acgaagagat agatttcaaa gttgagatag ataatccaga tttatgtaga
     2221 agatatgttg ctagaatggt tacagatgta aaaatagaac cttctccata ttggatgcaa
     2281 agaagactta cagaagcagg agtaagacct ataagtaaca tagtcgatat aacaaacttc
     2341 gtaatgttag agcttggtca accacttcat gcttttgata taaatcaagt agagactgga
     2401 agaatagtag taagaaatgc taaagatgga gagaaacttg taacattaga tgatgttgag
     2461 agaacattag acaaagatat gctagttata acaaatggag aaaaatcact tggtttagct
     2521 ggtgtaatgg gtggtgctaa ctcagaaata acttctaata cgaagactgt actttttgaa
     2581 agtgccaatt tcaaaccaga aaacataaga atgacagcta aaaaagttgg tattaggtca
     2641 gaagcatctt caagaaatga aaaagactta gaccctaatc ttgcagagat agcagcaaat
     2701 agagctgcac aacttgttga aatgttagga gcaggaaaag ttttaaaagg tgttgtagat
     2761 gtatatccaa ataaaccaga acctaaaaaa ttggtagtaa atcctcaaag aattaaccac
     2821 ctattaggtg tagatgtacc aatggagcag tttgtaggaa ttttagaatc attagagttt
     2881 aaatgtaatt tggtagctaa tgataaatta gaaatagatg taccaagctt tagaacagat
     2941 atggaacaag aagctgatgt atgggaagaa atagctagaa tttatggatt tgagaatata
     3001 ccttctgtac aattagaagg aaatacgaca gcaggaatta aaacttcaaa acaaaaattc
     3061 atggatgctt taaaagataa ttcaactgct gtaggattaa atgaaatatt aacatattca
     3121 tttgtaagcc ctaagggagt agataagata agagtaccag aaggtaatgc aaagagaaat
     3181 tttgttaaat tacttaaccc attaggagaa gaaacttctg taatgagaac tactttaata
     3241 ccaaatatgt tagatgtttt atcaactaat gtatctcata aaatagaaga agtatctgca
     3301 tttgagtgtg gacatatatt catcccacaa gattcagaat tacctaagga agaaaataga
     3361 atgtgtgtag gtatgtatgg taaagatgtt gacttctttg cactaaaagg aactatagaa
     3421 actatacttg taaatgtcgg atttaaaggt tatgaaattg aacctcaaga taataatact
     3481 accttccacc ctggtagatg tgctaaaata gtgtataata ataaatatgt aggtacatta
     3541 ggtgaattgc atccagatgt tatagaaaac tacaatctag gtcaaagagt ttatgtcgct
     3601 gaaatagata ttgactttgt atttgataat tctgatagaa caaagaatta tgtgccatta
     3661 ccaaaatatc catctacatc aagagatata gctcttattg ttaaagatga tgtatttgta
     3721 aaacaaatag aagatataat aaaggaaaat ggtcaaggat tagtagaaag ctacaagtta
     3781 tttgatgttt acaaaggttc tcaaatagaa gctggataca agtcaattgc atattcaata
     3841 acttatagaa gtaaagataa aactctaaca gatgaagatg tagctaaggt gcatgataag
     3901 atattaagtg aattaagcga aaaattaaat gctaacttaa gatcaaatta acaactaatg
     3961 tcgggggagg ttaggatgaa caaagtaatg gttaaaatcc atggcgctga atatccaatg
     4021 gttggagaca aatcagagaa gtttatgatt agtattgctg attttgtaga caaagaaatg
     4081 gataaaataa cacgtcaaaa cccgaaattg agcttatcag ttgctgcgat attaactgca
     4141 ttaaatatat cagacctttt atttgaatgt tcagatgaga atgagaagtt gattaaagcg
     4201 aatgaagaat tgagtaagaa ggttggagca tctaatgaag agcttcaatt agaaatcaaa
     4261 tctttaaagc ttacgatagc agaaaaagaa gcagaaaatc gtgaagctga aactaagatg
     4321 aaagagttaa ttgagattat agaaaataaa aagcaagaaa tattcgaact tagtaatacg
     4381 acagaaggtt ctagagcaga gttagatgct tataaaaaca agattgagga gttaagtact
     4441 caacttgaag aagcaaatga aagagctact atagctgaaa acttagcttc tgagtttcaa
     4501 aacaaagctt atgatttaca gttaaaatgt acagggttaa ataatgatgc aaaaaatgtg
     4561 gaataaacta aaagatttgc tgtctcttgt tttttgagac agcttctttt aatg
//
LOCUS       DAYAPA010000195         4579 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.200, whole genome shotgun sequence.
ACCESSION   DAYAPA010000195 DAYAPA010000000
VERSION     DAYAPA010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4579
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.200"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            193..1191
                     /locus_tag="ACGI59_003638"
     CDS             193..1191
                     /locus_tag="ACGI59_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HFG9741387.1"
                     /translation="MKILVFGARDYEEPVIKKWSEEHKDVQVDIYPENMTEENVVKAK
                     GYDGISIQQTNYIDNPYIYETLKDAGVKVIASRTAGVDMIHFDLVNENGLIVTNVPSY
                     SPNAIAELAVTQAMNLLRKTPLVKKKVCEGDYRWIAELLGTEVRSITVGVIGTGKIGA
                     TSAKLFKGLGANVIAFDQYPNSDLNDILTYKDSLEDLLKEADLITLHTPLLEGTKHMI
                     NKDTLAIMKDGAYIVNTGRGGLINTGDLIEALESGKIRAAALDTFETEGLFLNKKMNP
                     GELTDPEINKLLSMEQVIFTHHLGFFTSTAIENIVYSSLSSAVEVIKTGTATNRVN"
     gene            1553..2080
                     /locus_tag="ACGI59_003639"
     CDS             1553..2080
                     /locus_tag="ACGI59_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04002 family protein"
                     /protein_id="HFG9741388.1"
                     /translation="MEKMNKVTSKKTTYIVTSALFASIICLTIAYILHIPVGGNNGYV
                     HIGDAFIYLAATILPTNYAIAASAIGAGLADLSTGAAIWVIPTIIIKPILVLFFTSKS
                     DKIINKRNIVASVVAGIVGLVLYMFAEGIIIGSFTSAFVMSLLGLLQPIGSFIVFIIL
                     GMALDKLDFKKRYFN"
     gene            2107..2703
                     /locus_tag="ACGI59_003640"
     CDS             2107..2703
                     /locus_tag="ACGI59_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04100 family radical SAM protein"
                     /protein_id="HFG9741389.1"
                     /translation="MNILYTIENSIYVNITNTCPCSCVFCIRNEKDEVANSGSLWLEH
                     EPSVDEVKEAFNKYNLDDYDEIVFCGYGEPLMRINELIEIAKFIKEKSSIKIRINTNG
                     LSDLIHNKKTAILLKNVIDAVSISLNAPNKEAYNRVTQPKFGEKSFDYMLDFARDCKK
                     YIKEVAFSVVDEISPEEIEESKQLAKKLDIPLRVRHKN"
     gene            complement(2768..4351)
                     /gene="asnB"
                     /locus_tag="ACGI59_003641"
     CDS             complement(2768..4351)
                     /gene="asnB"
                     /locus_tag="ACGI59_003641"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HFG9741390.1"
                     /translation="MINLEEKYLTRKDTYMIKILEYNGHIRNWKVLCEELGIDSSLCR
                     DERERAILVEAYKTWGYDMANHMHGMFAFALWDTVEKKLFCLRDQFGVKPFYYYETEN
                     GKLLYGSSIRKIMEQPGFVKELNEDMLQIYMSFTYVAGENTFFRGVKKLMPGHYLVYQ
                     NKTVCIGRYWKPEFHPDHSKSIEEWTDEIHTTLQKIMPEVKSENETAELFLSGGVDSS
                     YILAMSDIEKTGSCGYEEERFDESKLAQQTANILGCKNSRYIITPEEYFASVPYVMYH
                     MEQPTGDASAIVFAIGCKAAAENTNICYSGEGADEFFGGYNIYHKAECYGENLKTFYI
                     GNTNIMNEGEKRKILKKYNPDVLPIEVVKGIYEETEELDTLTNMSNVDIQIWLEGDIY
                     FNIDKMSTAVGLEVRMPLTDTRIFDIASRIPSEYKVKGKENKIAFRTAASKTLPEEIA
                     FRNKLGFIVPIRIWMADDRYNKDIRDKFNSEIADKFFNIDEINEIFNDYISGNSDNWR
                     KIWMIYTFLVWYEIYFVKC"
BASE COUNT         1618 a          707 c          654 g         1600 t
ORIGIN      
        1 ttgataaatt ttagagtaat tattacatta aataaaaaat tactaaatat caaaataaca
       61 cagggaaaac aatttcaaaa ctaaattttt aaccttaaaa attttgtata aaaaatataa
      121 ccttatatgc tacatattat ttctaaatga aaaattacca taataattta ataattacag
      181 gagggtattg atatgaaaat actagtattt ggagcacgcg attatgaaga accagtaata
      241 aaaaaatggt ctgaagaaca taaggatgtt caagtggata tttatcctga aaacatgact
      301 gaagaaaatg tagttaaagc taaagggtat gatggtatat ctatacaaca aactaactat
      361 atagataatc cttatattta tgaaacttta aaagatgctg gggttaaagt tatagcttca
      421 agaactgcag gggttgacat gatacatttt gatttagtta atgaaaatgg acttatcgtt
      481 acaaacgttc cttcttattc acctaatgca atagctgaat tagctgttac tcaagctatg
      541 aaccttttaa gaaagactcc tctagtaaag aaaaaagtct gtgaaggtga ttaccgttgg
      601 atagctgaac ttcttggaac agaagttaga tctattacag ttggtgttat aggtacagga
      661 aaaataggtg ctacttctgc aaaattattc aaaggcctag gagctaatgt aattgcattt
      721 gaccaatatc caaatagtga tttaaatgat atattaactt acaaagattc tttagaagac
      781 cttctaaaag aagctgacct tataacatta catactcctt tacttgaagg aacaaaacat
      841 atgataaata aagatactct agctataatg aaggatggag cttacatagt aaatactggc
      901 cgtggtggtt taattaatac aggggattta atagaagcac tagagtcagg aaaaattaga
      961 gctgctgccc ttgatacatt tgaaactgaa ggattgttct taaacaaaaa aatgaatcct
     1021 ggagaattaa ctgacccaga aataaataaa cttctttcta tggaacaagt tatattcact
     1081 catcaccttg gtttcttcac tagtacagcg attgaaaata tagtttattc tagtttaagt
     1141 agtgctgtag aagttataaa aacaggaact gctactaata gagtaaatta gtattttata
     1201 aatatatttt ttcattaatt agctattatc taacaaaata gcctttaatt tcccaaatat
     1261 aagagagtca gctcagtata tattgagtag actctcttat attatttttt atattaaact
     1321 tgtactcttc ttattcatac caaattaaat ttatattaac ttattctaac tactcctact
     1381 ctatacattt tacctatttt tacttttcta attatataca tttgaatggg tacaatctgt
     1441 attttacaaa aaaaacacct aacaaactat gaaaattgta tgattaatat taatatattt
     1501 ataagaaaac ataacaatta tgttagctct ggatttttag ggagggagta tattggaaaa
     1561 aatgaataaa gttacttcta aaaaaactac ctatattgtt acttcagcat tatttgcatc
     1621 cataatatgc ctgacaatag catatatatt acatattcca gttggtggaa acaatggata
     1681 tgttcatata ggagatgctt ttatttatct agctgcaact attttgccaa caaactatgc
     1741 tatagctgct tctgcaattg gtgctggtct agctgattta tctacaggtg ctgctatctg
     1801 ggtaatacca actataatca taaaaccaat acttgtatta tttttcactt ctaaaagtga
     1861 caagataata aataagagaa atatagttgc ttcggttgta gcaggtatag taggattagt
     1921 tttatacatg tttgctgaag gtataattat tggaagtttt acaagtgcat ttgtaatgag
     1981 cttacttggc ttattacagc caataggaag ttttattgtt tttataatct taggtatggc
     2041 acttgataaa cttgatttta aaaaacgtta ttttaattaa gaatttaaat ttatggaggg
     2101 aaaattatga atatcttata tacaattgaa aatagtatct atgtaaatat aactaataca
     2161 tgtccatgta gttgtgtttt ttgtataaga aacgaaaaag atgaagttgc aaatagtggt
     2221 agtttatggt tggagcatga gccaagtgta gatgaagtaa aagaagcctt caataaatac
     2281 aatttagacg actatgatga aatcgtattt tgtggatatg gcgaaccatt aatgagaata
     2341 aatgaattga tagagatagc aaagtttata aaagaaaaaa gtagcataaa gataagaata
     2401 aatacaaatg gtctaagtga tttaatacat aacaaaaaaa cagctattct gttaaaaaat
     2461 gttatagatg cagtatcaat aagtcttaat gccccaaata aagaagctta taatagagtt
     2521 actcaaccaa agtttggaga aaaatcattt gattatatgt tagattttgc aagagattgc
     2581 aaaaaatata taaaagaagt agctttttca gtagtagatg aaatatctcc agaagaaata
     2641 gaagaatcta aacaactggc aaaaaaactt gatataccat taagagttag acataagaat
     2701 tagcaagtaa aattataaaa cattaagaat cacctgaatg gtatttcagg tgattcttcc
     2761 tcttgtatta acattttaca aaatagattt cataccacac taggaatgtg taaatcatcc
     2821 aaatctttct ccaattatca gagtttccac taatgtagtc attaaaaatc tcattaatct
     2881 catctatgtt aaagaattta tctgctatct cactattgaa tttatctcgg atatccttat
     2941 tatatcggtc atcagccatc catatacgaa ttggcacaat aaatcctaat ttatttctaa
     3001 aagcaatctc ttctggtaat gtttttgatg cagctgtacg aaaggcaatt ttattctctt
     3061 ttcctttcac tttatattct gatggtattc tggatgcaat atcaaatatt cttgtatctg
     3121 taagaggcat tctgacttcc aatcctactg cagtactcat tttatctata ttaaaataaa
     3181 tatctccttc caaccatatt tgaatatcta catttgacat gttagtaagt gtatctagtt
     3241 cttctgtttc ttcataaatt ccttttacca cttcaatagg aagcacatca ggattgtatt
     3301 tcttaagaat ttttcttttc tcaccctcat tcataatgtt tgtatttcca atataaaatg
     3361 tcttcaaatt ctctccataa cactctgcct tatggtatat attatatcca ccaaaaaact
     3421 catctgctcc ttctccagag taacagatat ttgtgttttc agctgctgcc ttgcatccaa
     3481 tagcaaaaac gatagcagat gcatctccag ttggttgctc catatggtac attacataag
     3541 gtactgacgc aaaatactct tcaggtgtaa ttatatatct cgaattttta cacccaagaa
     3601 tattagctgt ctgttgtgct aattttgatt catcaaaacg ctcttcctca taaccacatg
     3661 aacctgtttt ttctatatct gacattgcca atatataaga tgaatcaaca cctcctgata
     3721 ggaataactc tgcagtttca ttctctgatt ttacctctgg cataattttc tgaagtgttg
     3781 tatgaatttc atccgtccat tcctcaatag acttgctgtg gtcaggatga aattcaggtt
     3841 tccaataacg accaatacat actgttttat tctgatagac aaggtaatgt cctggcataa
     3901 gcttctttac tccacggaag aatgtatttt cacctgctac atatgtgaaa gacatataaa
     3961 tctgtaacat gtcttcattt aactctttca caaatcctgg ttgctccata atcttacgaa
     4021 tagatgaacc atagagaagc ttcccatttt cagtctcata ataatagaaa ggttttaccc
     4081 caaactggtc tcttaggcag aagagtttct tttccacagt atcccataat gcaaaagcaa
     4141 acattccatg catgtgattt gccatatcat atccccatgt cttatatgct tccactaaaa
     4201 ttgctcgttc acgctcatct ctacataatg aagaatcgat tccaagttct tcacaaagaa
     4261 ctttccagtt tcgaatatgc ccattgtatt ctagaatttt tatcatatat gtgtcttttc
     4321 gtgttaaata cttttcttct aaatttatca tatgtgtatc ctttcgtatt aaaacattta
     4381 acttctaaat ttattaaatg tttatatata tttattcact tataatcact gaaaaatcga
     4441 cttttttcaa agtaaattct ttttgaatta gtactaatct gctctttatt aatggcataa
     4501 atatgattat caagtattat cagtgttata acattgaatt ttttattctt cataaatatt
     4561 gcccccttta tattaccaa
//
LOCUS       DAYAPA010000196         4562 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.201, whole genome shotgun sequence.
ACCESSION   DAYAPA010000196 DAYAPA010000000
VERSION     DAYAPA010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4562
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.201"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            58..1440
                     /locus_tag="ACGI59_003642"
     CDS             58..1440
                     /locus_tag="ACGI59_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HFG9741391.1"
                     /translation="MLSKNIASKVLEKCLITGGDFAEIFEEDTINNSISLIDNKVENA
                     IGGRNYGIGIRIFKGLKSIYAYTSDNSLNSLLDVAYKAAVALGKLEDGKSVILNDSIK
                     INNIHNIKIYPNSIGNKDKVSVMKVAYKSAKEFSNDISQVSVSYLDKDQKILIANTEG
                     IYVEDRRIRTRLGISSIASKGNENQTGFEGPGGCKGFEIFEEIDPEYYAKESARVAHT
                     MLHAKNCPAGNMMVAIDNGFGGVIFHEACGHSLEATSVAKGNSVFADKLGQQIASTKI
                     TAIDDGTIPNYWGSMNIDDEGNPTQKNILIESGILKSYMIDKLNGRRMGMNPTGSSRR
                     QNYKFAPTSRMTNTYIAAGEDRPEDIIKSIPDGLYAKKMGGGSVNPVTGEFNFAVSEG
                     YLIKNGEIQEPVRGASLIGKGSDILMNIDMVGNNVKQAQGMCGSSSGSIPTNVGQPMI
                     RVKEITVGGR"
     gene            1452..2801
                     /locus_tag="ACGI59_003643"
     CDS             1452..2801
                     /locus_tag="ACGI59_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HFG9741392.1"
                     /translation="MELKNFKDILFKKALSEGFEECEIYYYTGENLSINIYEGEVEKY
                     NLDKSFGLSFRGKVNGKIGYSYTEILDDKAVDMLIKNVKDGVNTIENEDVQFIYEGDK
                     HYNDVKTYSKELENLEADKLIDLALEMERETKAYSDKVVNLSKCTISYSVSSNGISNT
                     KGLNLSNKTNMLIGFVVPIIEDNGQKYDGIGYNIANSIEEIKPCEIAKLGVDNALSKV
                     GGKSITSGKYKTILLNEAMVSLLSTFSGIFNADSAQKGLSLLKNREGDMIASPIVTIV
                     DDPLLENGMASTPFDDEGVATFKKEVVLNGKLITLLHNLKTANKAGVKTTGNGIKSSY
                     SSPISIYPTNFYIEKGDKSLDEIIKDIDEGLMVTSFAGLHSGANSVTGDFSLAAKGFY
                     IKEGKKVFPVEQITVAGNYFDLLKDIEAIGEDLEFPMSSIGSPSVVIKELSVAGKDE"
     gene            2951..3484
                     /locus_tag="ACGI59_003644"
     CDS             2951..3484
                     /locus_tag="ACGI59_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HFG9741393.1"
                     /translation="MSGAHYDMHKRNGYSSDEPYPEIKVLGPNKYYAELLMDDYAGVS
                     SEFTSVNQYLYHNFDLDETHRELSEMWINISITEMLHMEILAKTIRLLGGNPVYRGST
                     SSCGAYWNGGFVCYGNSICNRLKLDLHLEHVAINNYYKDISLIEDPYIKAILNRIILD
                     EKLHVSLFEKAIEKYCK"
     gene            3568..4377
                     /locus_tag="ACGI59_003645"
     CDS             3568..4377
                     /locus_tag="ACGI59_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFG9741394.1"
                     /translation="MYYVYVNDINIAVYDLNPSAKKTVLFIHGWPLGHKIFEYQTNIL
                     PKLGYRTVSIDLRGFGKSDATSGGYTYSQLADDIYKVVHAIGLKDFTLVGFSMGGAIV
                     LRYMSLFNGYGVSKLVLAAAAAPSFVQRPPEFPYGMTREDVNKLIAQACTDRPEMVTD
                     FGEKVFASNPPESFRRWFNDIGFSASGIGTIGTAVSLRDEELFNDLKCVRVPTGIFHG
                     KLDEICPYEFAVFMNEKIEDSILYTFEYSGHAIFYDELKLFNQEFLQFLEE"
BASE COUNT         1738 a          465 c          891 g         1468 t
ORIGIN      
        1 aacaagtaaa aagattgaat aaaagttaat taaaaattta aagtaggagg taaatttatg
       61 ctttcaaaaa atatagcttc taaggtatta gaaaaatgtc taattactgg aggagatttt
      121 gctgagattt ttgaagaaga cactataaat aattctatta gtttaataga taataaagta
      181 gaaaatgcaa taggtggaag aaattatggt ataggaataa gaatatttaa aggattgaaa
      241 agtatatatg cttatacaag tgacaattca ttaaatagtc tgttggatgt agcatacaaa
      301 gctgctgttg ctttaggtaa attggaagat ggaaaaagtg taatattaaa tgacagtatt
      361 aaaataaata acatacataa tataaaaatt tatccaaatt caataggaaa taaagataaa
      421 gtaagtgtta tgaaggttgc atataagagt gcaaaagaat ttagtaatga tatatctcaa
      481 gtaagtgtca gttatttgga taaagaccaa aaaatattaa ttgcaaacac tgaaggtata
      541 tatgttgagg atagaagaat aagaactaga ctagggataa gttcaattgc atctaagggt
      601 aatgaaaatc aaactggatt tgaaggtcct ggaggttgta aagggtttga aatatttgaa
      661 gaaatagacc cagaatatta tgcaaaagaa tcagctagag tagcgcatac tatgttgcat
      721 gctaaaaatt gtcctgcagg taatatgatg gtagcaatag ataatggatt tggtggagta
      781 atatttcatg aagcttgtgg acattctttg gaagctacat cagttgcaaa ggggaactct
      841 gtatttgcag ataaattagg tcaacagatt gcttccacaa agattacagc tatagatgat
      901 ggtacaatac caaactactg gggttctatg aatatagatg atgagggaaa tccaactcaa
      961 aagaatatac tgattgaaag tggaattttg aagtcttata tgatagataa acttaatggt
     1021 agacgaatgg gaatgaatcc tacaggaagt tctcgtagac aaaactacaa atttgcacca
     1081 acttcaagga tgactaacac ttatattgct gctggagaag atagaccaga agatattata
     1141 aagtcaatac cagatggact ttatgctaaa aaaatgggtg gtggttctgt aaatcctgta
     1201 acaggagagt ttaattttgc agtttcagaa ggttacttaa taaaaaatgg agaaatacaa
     1261 gagccagtta gaggtgctag tttaattgga aaaggtagcg atatacttat gaatatagat
     1321 atggttggga ataacgtaaa acaagctcag gggatgtgtg gctcatcatc aggaagtata
     1381 ccaactaatg taggccaacc tatgataagg gtaaaagaaa ttactgtagg tggaagatag
     1441 gagggataat tgtggaactt aaaaatttta aagatatatt atttaaaaag gctctaagtg
     1501 aaggttttga agagtgtgaa atatattatt atacaggaga aaatttaagt ataaatatat
     1561 atgaaggaga agttgagaaa tacaatcttg ataaatcttt tggtttatct tttagaggta
     1621 aggtaaatgg aaagataggt tattcttata cagaaatttt agatgataaa gctgttgata
     1681 tgttgataaa aaatgtaaaa gatggagtaa acactattga aaatgaagat gtacaattta
     1741 tatatgaagg agataagcac tataatgatg taaagactta ttctaaagag ctagaaaatt
     1801 tagaggcaga taagttgatt gatttagctt tagaaatgga aagagagact aaagcttatt
     1861 cagataaggt ggtaaattta tctaaatgta ctatatctta ttctgtatca agtaatggta
     1921 tatctaatac taaaggtctt aatttaagca ataaaactaa tatgcttata gggtttgtag
     1981 ttcctatcat agaagacaat ggacaaaaat atgatggaat aggatataat atagcaaatt
     2041 ctattgaaga aataaaacct tgtgagatag caaaacttgg tgttgataat gctttatcta
     2101 aagtaggtgg taagagtatt acatcaggta aatataaaac tatattgctt aatgaagcta
     2161 tggtatcttt gcttagcaca ttttctggga tatttaatgc agattcagct caaaaaggat
     2221 tatctttatt gaaaaataga gaaggagaca tgatagcatc tcctatagtt acaatagttg
     2281 atgacccact tttagaaaat ggtatggcat ctactccttt tgatgatgaa ggagtagcta
     2341 catttaaaaa agaagttgtt ttaaatggaa agcttataac tttacttcat aacttaaaaa
     2401 cggctaataa ggcaggtgta aagactacag gaaatggaat aaaatcttca tattcatcac
     2461 ctataagcat atatccaact aatttttata tagagaaagg agataaatct ttagatgaaa
     2521 ttataaagga tatagatgaa ggattgatgg ttactagctt tgcaggactt cattcaggtg
     2581 caaattcagt aacaggagac ttttctttag ctgccaaggg cttttatatc aaagaaggaa
     2641 aaaaagtatt tccagtggag caaattacag tagcaggaaa ctattttgac ctgttgaaag
     2701 atattgaagc tataggagaa gatttagaat ttccaatgag tagtataggt tcaccttcag
     2761 ttgttattaa agagctatca gtagctggaa aagatgaata attaaaaagt aattttgtgg
     2821 ataaaaggtc atgttaattt taattaatat ggctttttat tatttaagta gattttatta
     2881 tctaagttat atgaaaaagt tgtgatgcat acaattaata tcatacatta aatttaaagg
     2941 aggagatatt atgagtggtg cacattatga catgcacaag cgaaatggat attcaagtga
     3001 tgagccatat ccagagataa aagttttagg tccaaataag tattatgcag aattattgat
     3061 ggatgactat gctggagtat caagtgaatt tacgagtgta aatcaatatc tataccataa
     3121 ttttgattta gatgaaacgc atagagagtt aagtgaaatg tggataaata tatctataac
     3181 agaaatgtta catatggaga tattagcaaa gactataaga ttattgggtg gaaatcctgt
     3241 atatagaggt tctacaagtt cttgtggtgc atattggaat ggtggttttg tatgttatgg
     3301 aaattctatt tgtaacagat taaaactaga tttgcatttg gagcatgtgg ctattaataa
     3361 ttattataaa gatataagtc ttattgaaga cccttatata aaagctatat taaatagaat
     3421 aatcctagat gaaaaacttc atgtaagttt atttgaaaag gcaatagaga agtattgtaa
     3481 ataaatttga aaatttagtt attcatcatt ttattcatat atataaaata accatcatac
     3541 attttcttga agggagttgc tgttgatatg tattatgtat atgtgaatga tataaacata
     3601 gcagtatatg atttgaatcc gagtgctaaa aaaactgtac tttttataca tggttggccc
     3661 ttaggacata agatatttga atatcaaaca aatatattgc caaaactcgg atatagaaca
     3721 gtttcaattg atttaagagg ttttggtaaa tctgatgcaa catcaggagg atatacttat
     3781 agccaattag cagacgatat ttataaggtg gtacatgcta ttggattaaa agactttacc
     3841 cttgttggtt tttcaatggg aggagcaatc gtacttaggt atatgagttt atttaatgga
     3901 tatggagtgt caaaattggt attagctgca gctgctgcac catcatttgt acaaagacct
     3961 ccagaatttc catatggtat gacacgtgaa gatgtaaata aattgatagc acaagcatgt
     4021 actgatagac cagaaatggt aaccgatttt ggcgaaaaag tatttgcatc aaatccacca
     4081 gaaagcttta gaagatggtt taatgatatt ggttttagtg catcaggtat tggcactatt
     4141 ggaactgctg tatctttaag agatgaagaa ttatttaatg atttaaaatg tgtaagagtg
     4201 cctactggta tatttcatgg gaaattagat gaaatatgtc cgtatgaatt tgcagttttt
     4261 atgaatgaaa aaatagaaga ttcaatttta tatacttttg agtatagtgg tcatgctata
     4321 ttctatgatg agcttaaact atttaatcag gaatttttac agtttttaga ggaatagata
     4381 tattttatta tcacttagaa aaagagtaat attatgtgaa aagatttaag ctaaatttta
     4441 tgatgttttt tacactaaac aaatagggtt ttagaacttg taactaagtt ctgagaccct
     4501 attattatat attcctaaaa tttgttataa tttattcctt gtcattctta aaaatgtatt
     4561 aa
//
LOCUS       DAYAPA010000197         4529 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.202, whole genome shotgun sequence.
ACCESSION   DAYAPA010000197 DAYAPA010000000
VERSION     DAYAPA010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4529
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.202"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            105..404
                     /locus_tag="ACGI59_003646"
     CDS             105..404
                     /locus_tag="ACGI59_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9741395.1"
                     /translation="MKWIKWYSITCICIFIVVAFYMFIFPNKIETIDTSSAYSFVEKK
                     VPNSAVYQGYKKNPVDGTTTIYYSYDNSTHIVRLSHPEDYSRKINWDKVSNIRFD"
     gene            728..2383
                     /gene="ilvD"
                     /locus_tag="ACGI59_003647"
     CDS             728..2383
                     /gene="ilvD"
                     /locus_tag="ACGI59_003647"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HFG9741396.1"
                     /translation="MRSDIKKGIEGAPKRALMYGMGLTKEEIERPLIGIVNAQNEVIP
                     GHLHLDEIAEAAKNGVRMSGGLPLEFPAIGVCDGIAMGHVGMNYSLASRELIADSIEA
                     MAMAHGFDALVLIPNCDKIVPGMLMAAARLNIPSIVVSGGPMLPGKKNGKVYDFNSAM
                     EGVGACKDGTVSEEELEELAMNSCPGCGSCSGLFTANSMNCLTEALGMGIPYNGTAAS
                     HSGERKRIAKYAGMYVMELLKNDIKPRDILTIDAFKNAIAVDMAMAGSTNTVLHLPAI
                     AYESGIELNLDFFDEISEKTPCLTKLSPSGKHHIEDLHMAGGIPAIMNELSKINGINL
                     DCKTVTGKTIGENIRNCEIENEEVIHTLKNPYSNQGGLAILKGNLALNGAVVKKSAVA
                     EEMLVHEGPARVFNSEEEAVNAIFGKKINKGDVIVIRYEGPKGGPGMKEMLSPTSAVA
                     GMGLDKHVALLTDGRFSGATRGASIGHISPEAMEGGLIGLVEEGDIISINIPDKKLEL
                     KVDEVEIENRKLKFKPLEPKIKHGYLSRYAKLVTSANTGAVLK"
     gene            2727..3356
                     /locus_tag="ACGI59_003648"
     CDS             2727..3356
                     /locus_tag="ACGI59_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFG9741397.1"
                     /translation="MNREEKSKNSKEKIIQSAFSLFSSKGYDSTSTQDIINLSGLSRG
                     AMYHHFKTKEDILRSVTKELYSQMNNFLEHLVADDTLTANEKIIELVVHSANDYTRRK
                     MVHCSWLEKIPFALIEEVRNLNNVVAPNIAKIIKQGVENKEFSCEYPEELAEMLVFSI
                     DILLDPVLFKREYSEVCNRLDFLLFMLKKMDIPLIDEYGIQKFKDLFKQ"
     gene            3382..4194
                     /locus_tag="ACGI59_003649"
     CDS             3382..4194
                     /locus_tag="ACGI59_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFG9741398.1"
                     /translation="MFYNAKSCQMMIGSNTVNYIEFGSGKKTLIILPGLGDGLFPLHG
                     KIQAIAFAFRYKQFTKDYKVYVFSRKNQITEKYSTRDMAKDQADIMKKLGIMKAEVMG
                     VSQGGMIAQYLAIDYPELVEKLVLAVTSSKQNDTIQNVICSWIDMAKKQNYNDLMIDT
                     AKKSYSERYLKKYQLFIPFLGKVGKPKDFKRFIIQATSCIEHNAFSELNKITCPTLII
                     GGANDKIVGNNASFHLVEKIKKSEIFIYEGLGHATYEEAQDFNERVLEFLNK"
BASE COUNT         1760 a          503 c          817 g         1449 t
ORIGIN      
        1 tctacaatta tatagttagg gatataaaaa ttttatcgta tgcataaata ttataaatat
       61 atggaaataa taatagtata ttttggtaaa gtaggaggaa ttttatgaaa tggattaaat
      121 ggtatagtat tacatgcata tgtattttta tagtagtagc tttttatatg tttatatttc
      181 ctaataaaat agaaactatt gatacttcat ctgcatatag ctttgttgaa aagaaagtac
      241 caaatagtgc tgtgtatcaa ggatacaaaa agaatccagt tgatggtact actacaattt
      301 attacagcta tgataattca actcatatag ttagattatc acatccagaa gactattcaa
      361 ggaagattaa ttgggataaa gtttcaaaca tcagatttga ttaatcttta atatttataa
      421 aagtctatat atctaaggca aatatattat taatcttatt catagttgag cactttctat
      481 tatagaagtg cctcttctga tttatatcat aaaatattac tcaacaattt gtaaaatata
      541 ttagtttata aaatatattg aaaagataat attatataat acgattgtgt aagatattga
      601 taaaaaatcg taataaattt tcattagtat atgctaatat ttttttacaa ttttcgtaat
      661 taattaatag aaaaatatat tataataata ttatgaaaat ttaaaaaagt aagaaagagg
      721 taatattatg agaagtgata taaaaaaagg aatagaagga gctccaaaga gagcgttaat
      781 gtatggaatg ggtcttacta aagaagaaat tgaaagacca ttaataggta tagtaaatgc
      841 acaaaatgag gtgattcctg gtcatctgca tcttgatgaa atagcagaag ctgcaaaaaa
      901 tggagtaaga atgagtggtg gtttgccatt agaatttcct gctatagggg tatgtgatgg
      961 tatagctatg gggcatgtcg gaatgaatta ttccctggct tcaagagaat taatagcaga
     1021 ttcaatagaa gctatggcta tggcacatgg ttttgatgct ttggtactta tacctaattg
     1081 tgacaagata gtgccaggta tgttaatggc tgctgcaaga cttaatatac caagtattgt
     1141 tgtgagtgga gggcctatgc ttccaggtaa aaaaaatgga aaagtatatg attttaactc
     1201 tgcaatggag ggtgttggag catgtaaaga tggaacagta agtgaagaag aattagaaga
     1261 gttagccatg aactcttgtc ctggatgtgg ttcatgttct ggtcttttca cagcaaatag
     1321 catgaattgt ttaacagaag ctttaggcat gggtatacca tataatggta ctgctgcgtc
     1381 acattctgga gaaagaaaaa ggatagcaaa atatgcaggt atgtatgtta tggagttact
     1441 taagaacgac ataaaaccta gagatatttt aacaatagat gcttttaaaa atgctatagc
     1501 tgtggatatg gcaatggctg gttctacaaa tacagtactt cacttacctg caatagctta
     1561 tgaatcagga atagagctta acttagattt ttttgatgaa ataagtgaaa aaactccttg
     1621 tttaacaaaa ttaagtccaa gtggaaaaca tcatattgaa gatttacata tggcaggagg
     1681 aataccagct ataatgaacg aactttcaaa gataaatgga ataaatttag attgcaaaac
     1741 cgtaacaggc aagactatag gggaaaatat aagaaattgt gaaatagaaa atgaagaagt
     1801 aatacataca ttaaagaatc catatagtaa ccaaggtggg cttgcaatat tgaaaggaaa
     1861 tcttgctcta aatggagctg ttgtaaaaaa gtcagcagtt gcagaagaaa tgttagttca
     1921 tgaaggacca gcaagagttt ttaattcaga agaagaagct gtaaatgcta tttttggtaa
     1981 aaaaataaac aaaggtgatg ttatagttat aagatatgaa ggtccaaagg gtggtccagg
     2041 aatgaaagaa atgctatctc ctacatcagc agttgcagga atgggacttg ataagcatgt
     2101 agcacttctt actgatggtc gtttttcagg ggcaactaga ggggcatcca taggccatat
     2161 ttctccagaa gctatggaag gtggtttaat cggactagtt gaagaaggag atataatttc
     2221 tataaacata ccagacaaaa aattagagtt aaaagtagat gaagttgaaa tagaaaatag
     2281 aaaattaaaa tttaaacctt tagaaccgaa aataaagcat ggatacttaa gtagatatgc
     2341 taaactggta acatcagcaa atacaggagc agttttaaaa tagtagacta tatttatgta
     2401 aattagaaaa agttaagaaa atattaattg gatatcttag gtgttatagc actaatgagg
     2461 tatccttttt attttgtatg aaaaacatag caaaatcttg attatctttg aaaacaatac
     2521 atctctatta taaggaatac gagtacagca tagagatgta tcttttagac cttgttgagg
     2581 taatacaact ttttaaaagt ttgatgtgga atgcttttcg ttccttctga actatgaaga
     2641 aattgaaagt aagttaattc ttgacataat aaatattaaa atgtataata aaaaacaaac
     2701 agacagtctg tttttaggag ggaaatatga atagagaaga aaaaagtaaa aatagtaaag
     2761 aaaaaattat ccaatcagca ttttcactat tttcgtctaa aggatatgat tcaacatcta
     2821 cacaagatat tatcaattta tctggtctat ctagaggtgc aatgtatcat cactttaaaa
     2881 ctaaagaaga tatactgaga agtgtcacaa aagaacttta ctcacaaatg aataattttt
     2941 tagagcatct tgttgctgat gacaccctta cagcaaatga aaaaataata gaattggttg
     3001 ttcatagtgc gaatgattac acacgtagaa aaatggtaca ttgtagctgg ttagaaaaaa
     3061 tcccattcgc tttaatagag gaagttcgta atctcaacaa tgtagttgca cccaatattg
     3121 ctaagataat taaacaaggt gttgaaaata aagaattttc ttgtgaatat ccagaggaat
     3181 tagctgaaat gctcgttttt agtattgata ttttacttga ccctgtatta tttaaacgtg
     3241 aatacagtga agtatgtaat aggttggatt ttttattatt catgttaaaa aaaatggata
     3301 ttcctctaat tgatgagtat ggtattcaaa aatttaaaga tttatttaaa caatgataat
     3361 aggaaaagtg aggaatagac tatgttttat aatgcaaaaa gttgtcaaat gatgatagga
     3421 agtaatacag tgaattatat tgaatttggt agtggtaaga agacactaat tattttacca
     3481 ggattaggag atggtttatt tcctttacat ggaaaaatac aggcgattgc ttttgctttt
     3541 agatataagc aatttacaaa agattataag gtttatgtat ttagtagaaa aaatcaaatt
     3601 acagaaaaat attccacaag ggatatggct aaagaccaag cagatataat gaaaaaactt
     3661 ggaattatga aagcagaagt aatgggtgtt tctcaaggtg gaatgatagc tcaatatctg
     3721 gcaattgatt atcctgagtt agttgagaaa cttgtgttgg cagttacttc ttcaaagcaa
     3781 aatgatacta tccaaaatgt tatttgtagt tggattgata tggcgaagaa gcagaattac
     3841 aatgatttaa tgattgacac agcaaagaaa tcctattctg aaagatattt aaaaaaatac
     3901 caattattta ttcctttttt aggaaaagta ggtaaaccaa aggattttaa acgatttatt
     3961 attcaagcga cctcttgcat agaacataat gccttttccg aattaaataa aataacatgt
     4021 ccaaccttaa ttataggagg tgctaatgat aaaattgttg gaaataatgc ttcttttcat
     4081 ttagtagaaa aaattaaaaa aagcgaaata tttatctatg agggattggg acacgctaca
     4141 tatgaagaag cacaggattt taatgaaaga gttttagagt ttttaaataa gtaaacacct
     4201 ttgttaaaaa tatacatatg aagattggaa atttaatgtt aaaaatagaa acatgaaaat
     4261 atgcttaact ggtattttta ctattcataa ccaatttttg atacattatc tactataaat
     4321 acaaatatag cttcaatgtg attatatatt gttgtattgg taaagcactt atacaaacag
     4381 aggaattttg taaattcata ttatacccac atttgttaac ttatgaaaat ataatcaaaa
     4441 tttttatgag ctttttatat aaaaaatata atgtttgtat acaaaaatat taaagacatt
     4501 ttagtaagtt ttgtatataa gcaaacatg
//
LOCUS       DAYAPA010000198         4492 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.203, whole genome shotgun sequence.
ACCESSION   DAYAPA010000198 DAYAPA010000000
VERSION     DAYAPA010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4492
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.203"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(<1..236)
                     /locus_tag="ACGI59_003650"
     CDS             complement(<1..236)
                     /locus_tag="ACGI59_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFG9741399.1"
                     /translation="MNYYELLRIKKERHKNKNFLIIDGEKYTYENILSYSEELGKQIS
                     LGENIPILIYSKNIMFQLISFFAINYSKNIPIIC"
     gene            complement(240..1376)
                     /locus_tag="ACGI59_003651"
     CDS             complement(240..1376)
                     /locus_tag="ACGI59_003651"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HFG9741400.1"
                     /translation="MKEVFILGGLRSHIGLKNGIFQFVQPELLGASVLKDLIKKYEID
                     KIDEIICGNAVGTGGNIARLMTLTAGVSNEVPAFTVDMQCASAMMSIDIAFSKVKSGQ
                     CDLIIAGGFESSSLQPMRTYHKNDKRYNTNNPNYTVAQFSPDDNSQNSMLEGAERVAE
                     LYQIEKADLDFWVKESHKRAKEAREEKILEDIISPINNSTYDEGIRDKMSQRLLDRMP
                     SILGKETITNAANSCLINDGASFIIICSKKYLEHVKKKPKAKIINTCTIGTDATLSPT
                     SAIQAMDKLLEIESLNYLDVSAVEFNEAFAVIDVLFQRKYPELIDRYNIFGGALAYGH
                     PYGASGAIIALHLLKALEKTKGRYGICSIAAAGGLGSALLLERV"
     gene            complement(1408..1953)
                     /locus_tag="ACGI59_003652"
     CDS             complement(1408..1953)
                     /locus_tag="ACGI59_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HFG9741401.1"
                     /translation="MKNTKVNIQDLTKMSICVALLCISSYIYIPLPFTPAGVTAQTIM
                     INLIALILTPRQAFSTVGVYIMIGLIGIPVFGGGTSGIGKLLSPTGGFYFGFLFAALI
                     ISLLKGRNNDIKRYILITIFVGMPIIYFMGTVFMCYFNQMTVEAALFAAVVPFLIGDT
                     IKSVIASFLGTKLNNVLRRNL"
     gene            2277..3047
                     /locus_tag="ACGI59_003653"
     CDS             2277..3047
                     /locus_tag="ACGI59_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9741402.1"
                     /translation="MKVNENNIDEELDELSRKVYLESPDFWISISDKIIDKVFDEMLL
                     FFAVKYNYFNIVCFAIENNYLDLNMPSKNKKYTNIMEHLLDTSKQNEDKKIYNYLLSL
                     NKGNNLDERPILDELNCNISSDTFEKKDIYLPKYLCPSCNSNIFDTGYKVSNDITYKF
                     SSKEAEPIEMCSEVSSIRCCNCDELLEDITLDKLYNLCKIQNCNNCGSNLTKVGIIAK
                     KKMNFDENTEKFKDVSTFYCCGNCDSEISLSQREHFKI"
     gene            complement(3501..3929)
                     /locus_tag="ACGI59_003654"
     CDS             complement(3501..3929)
                     /locus_tag="ACGI59_003654"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFG9741403.1"
                     /translation="MIKKLNSKDINKIMEIWEKSTIKAHDFISKEYWQNNYNTVKNEY
                     IPISDTFVYDDGDEIKGFISIIDKNFIGALFIKPKYQNLGIGGKLLDYATKKYKSLSL
                     AVYKDNKKAVVFYNKKGFNIVKEQVNEDSGFKEYIMEYSK"
     gene            complement(4125..4358)
                     /locus_tag="ACGI59_003655"
     CDS             complement(4125..4358)
                     /locus_tag="ACGI59_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFG9741404.1"
                     /translation="MMILIQSRGKMKCKELSEELEVSERQIKSYKMYLEQAGIFINST
                     PGIYGGYEIDKCNSISLIKLLDSEVSIFISTKW"
BASE COUNT         1556 a          673 c          478 g         1785 t
ORIGIN      
        1 tgacaaataa taggaatatt ttttgaataa ttaatagcaa aaaaactaat caactgaaac
       61 attatatttt tagaatatat taaaattggg atattttcac ctaaagaaat ttgctttcct
      121 agttcctcac tataactcaa tatattttca taagtatatt tttctccatc tataattaaa
      181 aaatttttat ttttatgcct ctcttttttt attcttaaaa gttcataata gttcataatc
      241 tacacccttt caagcagtaa agctgaacct agaccaccag ctgcagctat agagcaaatt
      301 ccatatctac ccttagtctt ttctaatgct tttaaaagat gtaatgcaat aattgcacct
      361 gaagcaccat atggatgtcc atacgctaaa gctccaccaa atatattata tctatctatt
      421 aactctggat actttctttg aaacaacaca tctataactg caaatgcttc attaaattca
      481 acagcagata catctaaata attaaggctt tctatctcta aaagtttatc cattgcttgt
      541 atcgctgatg ttggacttaa ggttgcatct gttcctatcg tgcatgtgtt aataattttt
      601 gctttaggct tcttttttac atgttctaaa tacttcttag aacatattat aataaatgaa
      661 gctccatcat taataagaca tgaatttgct gcatttgtaa tagtttcttt tcctaaaatt
      721 gatggcatcc tatctaataa cctttgactc atcttatcac gtataccttc atcataggta
      781 ctattattta taggagatat aatatcttca agtatttttt cctctcttgc ttcttttgct
      841 cttttatgac tttcctttac ccaaaaatcc aagtctgctt tctcaatttg atataactct
      901 gctactcttt ccgcaccttc gagcatacta ttttggctat tatcatcagg agaaaattga
      961 gcaactgtat aatttggatt atttgtatta tatcgcttat cattcttatg atatgtgcgc
     1021 attggttgca gagaactact ttcaaaacca cctgcaataa ttaaatcaca ctgaccagat
     1081 ttaacctttg aaaaagctat gtctatactc atcatagcag aagcacactg catatccaca
     1141 gtaaaagcag gtacttcatt tgacactcct gctgttaaag tcattaatct agctatattc
     1201 cctcctgtcc caactgcatt tccacatata atctcatcaa ttttgtcaat ttcatacttt
     1261 ttaattaaat cttttaaaac acttgctcct agtaattcag gttgaacaaa ttgaaaaatt
     1321 ccatttttta gacctatatg acttctcaat cctcctaata taaaaacttc cttcatacat
     1381 tacacccttc cttatattat agatttatca tagatttctt ctgagtacat tgttcagttt
     1441 agtgcctaaa aaagatgcaa taactgactt tattgtatct ccaattaaaa atggtactac
     1501 tgctgcaaaa agtgctgctt ctactgtcat ttgattaaaa tagcacataa atactgttcc
     1561 cataaaatat ataataggca ttccaacaaa aattgttatt aatatatatc tcttaatatc
     1621 attatttcgt ccttttaata aactaataat tagtgctgca aacaaaaacc caaaataaaa
     1681 accacctgtt gggcttaata actttccaat accagatgta cctccaccaa atactggaat
     1741 cccaattaaa cctatcatta tatatacacc aactgtacta aatgcttgtc ttggtgttaa
     1801 aatcaaagct attaaattta tcattattgt ttgtgctgtt actcctgctg gagtaaatgg
     1861 caatggtata tatatgtaag atgatataca caataatgct acacatattg acattttagt
     1921 taaatcttga atatttactt tagtattttt catttttata attcccttca tatgtcttat
     1981 ttcatttagt ttatatgtac atatacaaag cttctattaa agctttgttt tagtataaaa
     2041 taaagacgtt tttttgtcaa ctattataat tatttaggtt tacatttaat ttataagtaa
     2101 actttatact gttactttat attttaatag tcatttataa aactttgctt aaaaacagct
     2161 ctatttagac tatacttgta aattataata tgatatattt ttatataaga ttttatttta
     2221 tataaaaaat agtaacaaat agtttataca caagatattt aaaaggaagt gatgatatga
     2281 aagttaatga aaataatata gatgaagagt tagatgaact tagtaggaaa gtttacttag
     2341 agtcaccaga tttttggata agtatttctg acaaaataat agataaagta tttgatgaaa
     2401 tgttattatt ttttgcagtt aaatataact atttcaatat agtatgcttt gctattgaaa
     2461 acaattatct ggatttaaac atgccttcaa aaaacaaaaa atacactaat ataatggagc
     2521 atttgttaga tacatcaaaa cagaatgaag ataaaaaaat ttataattat cttctaagct
     2581 taaataaagg taataaccta gatgaaagac caattttaga tgaattaaac tgtaatatta
     2641 gtagtgatac ttttgaaaaa aaagatatat atcttccaaa gtacttatgt ccaagttgta
     2701 attctaatat atttgataca ggttataaag tatcaaatga cataacatat aaattttctt
     2761 ctaaagaagc tgaacctatt gaaatgtgta gtgaagtttc ttctatcaga tgttgtaatt
     2821 gtgatgaatt attagaagat attacacttg ataaactata taacctatgc aaaattcaaa
     2881 actgtaataa ttgtggttct aatttaacta aggtaggaat tattgcaaag aaaaaaatga
     2941 actttgatga gaatactgaa aaatttaaag atgtctcaac attttactgt tgtggaaatt
     3001 gtgactctga aataagctta tctcaaagag aacattttaa gatttaaata tttataggct
     3061 aaagtaataa gattacatta ttagatactg tataagtttt ttaaatctgt aaaaataaat
     3121 acacttataa gaattaaaat tgctatgaaa ctagttcttg atgaactaat ttcatggcaa
     3181 ttttctataa tctaataatt attaaatttt aacaaacctt tattagcatt ccataattat
     3241 ttaaaattct ttcaatactc tatagtttat aggtgaaagt atctttaata ttatatcaaa
     3301 cttattatct ttaaatagta aatttgttaa atctgaaata caccatacaa ttttattttc
     3361 ctcttttgta gctatatcaa ttctgattaa cttacatcta taataaatat ctttatcttc
     3421 atatagtcta ttttaaaaat tactcttaaa aaatcaacta gaaaataaat taattatcct
     3481 tttaatagaa tgtaatcata ttatttacta tattccatta tgtactcttt aaatcctgaa
     3541 tcttcattta cttgttcttt tactatatta aaaccttttt tattataaaa cacaactgct
     3601 tttttattat ctttatatac tgctaagctt agacttttat attttttagt tgcataatct
     3661 aaaagtttac ctccgatacc tagattttgg tatttgggct ttataaataa agctcctata
     3721 aagtttttat ctattatgct tataaatcct tttatttcat ctccatcatc atatacaaat
     3781 gtatctgata taggtatata ttcgttttta acagtattat agttattttg ccagtattct
     3841 ttacttataa agtcatgtgc tttgattgta cttttttccc atatttccat gattttattt
     3901 atgtctttac tgtttaattt ctttatcata aattttgttt cacctcattt ttgtattgat
     3961 tttataatgt ttgtaggtaa gtgtatttta tttagatatg atttagttaa tataaggtgg
     4021 atgttttagt atatgctatt gttgattcta caattttata tactgcttta ttaatataaa
     4081 ataagtttct aaatactcgt tgatattgct aatattctac ttatctacca cttagttgat
     4141 ataaaaattg aaacttcact atctaataat ttaattaatg atatgctatt acatttatct
     4201 atttcataac caccataaat cccaggtgtt gaatttataa atataccagc ttgctctaaa
     4261 tacatcttgt aactttttat ttgcctttca cttacttcca actcttctga taattctttg
     4321 catttcattt tgcctctgct ttgaattaat atcatcattc taagtgtatt agctaattta
     4381 ctaatgagta acccccccct aataacatta attgatattt ttattattta tttctacaca
     4441 tatgtacata taacctcttt atattttata taagtagaat ttttttataa tt
//
LOCUS       DAYAPA010000199         4421 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.204, whole genome shotgun sequence.
ACCESSION   DAYAPA010000199 DAYAPA010000000
VERSION     DAYAPA010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4421
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.204"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            467..946
                     /locus_tag="ACGI59_003656"
     CDS             467..946
                     /locus_tag="ACGI59_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFG9741405.1"
                     /translation="MDKTTFTNNILESEQTLYRVSKSILGNDQDCEDAVNNAILKAYE
                     KLDSLKEEQYFKTWLIRIVINECNSLRRKRLKSLSFEDVFKNKKIDEKDDYSDLYTAI
                     QSLPKKIKIPIVLYYIEGYSVDEVKEILDIPQGTVKSRLSRGRRLLKTKLENTEVII"
     gene            943..1722
                     /locus_tag="ACGI59_003657"
     CDS             943..1722
                     /locus_tag="ACGI59_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9741406.1"
                     /translation="MNKNILNNSFPEVPESFHNSLSKTLNSLPEREENYKMINNKTHK
                     LPFRKGLVATLAITLILSTTALAVGHIYSLVGKSSTKATYTSIPTTEQVKKDFNFTPN
                     IVSEFSNGYKFKGAYTVNKKAFDKENNVLGKSKALSFEYKNGNDEISLDTANNVLGKS
                     SDNLKVVDTYKDINISYEAFAQKFIPEGYKMTEQDKIDEKSGKYTFSVGPNNESDKVE
                     VNEFKFLTWKQDGVYYSFTVQDSNLSMNDLVKMAHEVIDAK"
     gene            complement(1972..2472)
                     /locus_tag="ACGI59_003658"
     CDS             complement(1972..2472)
                     /locus_tag="ACGI59_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HFG9741407.1"
                     /translation="MIEGIKFIKAEEKYIYSYWQTFDKIAKERKYLAMDEAFPFEETV
                     EFIKNIINKNLPQLFIIDLESDNCIGWCDVLPKTEKVGYLGMGILKEYREKGIGSSLL
                     KQIIDLSKEYGYEKIELDVFKSNSRAIHVYKSLGFVEVNTISSGFTWNDRPVKEEVIQ
                     MELTLI"
     gene            complement(3084..3791)
                     /locus_tag="ACGI59_003659"
     CDS             complement(3084..3791)
                     /locus_tag="ACGI59_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFG9741408.1"
                     /translation="MIFTKSNKYDKDFLMKNMMGPNCIKILEELTSKIKLEKGMRILD
                     LGCGKGISSIFLAKEFDATVFATDLWIEPTENYKRFKEFKLDDKIFPIQAEAHELPYA
                     EGFFDAVISIDSYHYFGNKEGFLDNHISPLVKEGGILAMAMPGLKEDFVDCIPDELIP
                     FWQDNMNFHSITWWNKLWSESESVIVEKCEALNCHDEAWKDWINCDNSYAINDKKMME
                     VENGKYFNTISLIARTK"
BASE COUNT         1641 a          653 c          462 g         1665 t
ORIGIN      
        1 gttcatctct atctaaaata atgacatttc taactcttaa ttctcattta agtttcattg
       61 ttttttctta aaagcctact tttagtcaag atactgttat ctttactcat tatttaaaga
      121 acttatttta aatctgtaca tgaagtaaat aatccaaaag cattgttatt ctaactactc
      181 aacattaaat acttactatt ttctaattac tcaatataaa gacttactat aaaactaaat
      241 acataaatgg cttctaaagt ttaatatatc tacatggctt tatatatgac aggaaaaata
      301 ctagaaaaaa tatattatag aatacttgta tataataggc aaaactttca aacttaaatt
      361 tatcaaataa tcataaaaaa agtgaaccat ctatagtaaa attttatcta ataagtgaaa
      421 ggcaaaaaag tagcgctacg atttgcatag aagggaagga acttagttgg ataaaactac
      481 atttacaaac aatatacttg aatcagaaca gactttgtat cgagtatcta agtctattct
      541 aggaaatgac caagactgtg aagatgccgt caacaatgct attttaaaag catatgaaaa
      601 gttggattct cttaaggaag aacaatactt taaaacatgg ctgattagaa tagtcataaa
      661 tgaatgtaat tcattaagac gcaaacgatt aaaatctcta tcatttgaag atgtcttcaa
      721 aaacaaaaaa attgatgaaa aagatgacta tagcgatttg tatactgcta ttcaaagtct
      781 acctaaaaaa attaaaattc caatagtcct ttactatatt gaaggttatt cagtagatga
      841 agttaaggaa atattagata tacctcaagg aactgtaaaa agtagattat caagaggaag
      901 aagattatta aaaactaaat tagaaaacac agaggtaatc atatgaataa aaatatatta
      961 aataattcgt ttccagaagt tccagaaagt tttcataata gtttaagcaa aacactaaat
     1021 agtttaccag aaagagagga aaattataaa atgattaata ataaaacaca caaattacca
     1081 tttagaaaag gcttagttgc aactttagcc attactttga tattgagcac aacagccctt
     1141 gcagtaggac atatatatag tttagttgga aaatcaagta ctaaggctac ctatacatct
     1201 atcccaacta cagaacaagt aaaaaaagac tttaacttta cccctaatat agtttctgaa
     1261 tttagtaatg gatataaatt taaaggagca tatactgtaa ataaaaaagc atttgacaaa
     1321 gaaaataatg ttttaggaaa atcaaaagca cttagttttg aatataaaaa tggcaatgat
     1381 gaaatatctc ttgacacagc aaataatgta cttgggaaga gttctgacaa tctaaaagtt
     1441 gtagatactt acaaagatat taatatttct tatgaagcat tcgcacaaaa gtttatacca
     1501 gaaggttata aaatgacaga acaggataaa attgatgaaa aatcaggcaa atacacattt
     1561 agtgtggggc caaataatga atcagacaaa gttgaagtta atgaatttaa gtttttgaca
     1621 tggaaacaag atggagttta ctattctttt acagttcaag actcaaattt atctatgaat
     1681 gacttagtta aaatggctca tgaagtaata gatgcaaaat aaacataagt aataataagt
     1741 tgtttatgag tttattattt atgactttaa tttttttatt agtaaacatg gttgtatctt
     1801 ccatctttat attttcttta aatttaatat ataaaatttt agatatttag ctaccttcaa
     1861 aagaagcatt attaaaaagc ccttaaaaat atttctttta tatggaattt taagggcttt
     1921 aactttattt tatacaagtg tatatggaat tttaaagact ttaactttat tttatataag
     1981 tgtaagctcc atttgaatta cttcttcttt tacaggtcta tcattccatg taaaaccact
     2041 agatattgta tttacctcta caaaccctaa agatttataa acatgaatag ctctactatt
     2101 acttttaaat acatcaagct caattttttc atacccatat tcttttgata aatcaataat
     2161 ttgttttaaa agactacttc cgataccttt ttccctatac tctttaagaa tacccatgcc
     2221 taaataacca actttttcag ttttaggtaa aacgtcacac caaccaatac agttatctga
     2281 ttctaaatca attataaaca gttgtggtaa gtttttattt ataatatttt ttataaattc
     2341 aactgtttct tcaaaaggaa aggcttcatc catagctaga tatttcctct cttttgctat
     2401 tttatcaaaa gtttgccaat acgaataaat atacttttct tctgccttta taaattttat
     2461 gccttctatc aacttaacat ttcccctttt taatttttga ttgtagttaa cttattttat
     2521 atgattctag tttataatgc tatataaatt actatatatt agcttaaaat atctttgtat
     2581 atttctataa aattaaaacc aatatatcta tctcttgcat ctctattaaa ataattttta
     2641 atagtataat aaatatagtt ctttaatctt ttgggtatat atacttaatc attttgaaat
     2701 ttgatttaaa gtataataaa atatataaaa aaatatataa aatggtacta taaaattttt
     2761 aatcttcatc aatacctttt ttgctttcat tgttaaaact attttttcta ttagtttatt
     2821 tcttataaaa caaacattta gtattaaaat atatatcaca caatagtatt tgcaatttaa
     2881 ctttaaaaaa ataaaaatac ccaactgacc taagcaactc gattgggtat aaataatcta
     2941 aaaaatcatt tttgtataaa ctctttattt aacttacatt tttatttacc ttctacttta
     3001 attcaactta tatttctatg cttcttatac tctcattcaa cttatatttc tacttatctt
     3061 atacttttat aaattcatca caattattta gttctcgcta tcaaagaaat tgtattaaaa
     3121 tactttccat tctctacttc catcattttt ttgtcattta tagcatatga attatcacaa
     3181 tttatccaat ccttccaagc ttcatcatga caatttaaag cttcacattt ctctacaatc
     3241 acagactccg attcagacca aagtttattc caccatgtaa ttgaatggaa gttcatatta
     3301 tcttgccaaa atggaatgag ttcatctggt atacaatcca cgaaatcttc ttttaatcca
     3361 ggcattgcca tagcaagtat tcccccctct tttacaagag gtgatatatg attgtctaaa
     3421 aatccttctt tgtttccaaa atagtgataa gaatcgatac ttatcaccgc atcaaagaaa
     3481 ccttctgcat aaggtagttc atgtgcctct gcctgtattg gaaatatttt atcatccaat
     3541 ttaaactctt taaatctttt atagttttca gttggttcta tccataaatc tgttgcaaat
     3601 acagtagcat caaattcctt tgctaaaaat atcgaagata tacccttacc acaacctaag
     3661 tctaaaatac gcattccctt ttctagtttt atcttacttg ttaattcttc caatattttt
     3721 atacaattag gtcccatcat atttttcatg agaaagtcct tatcgtattt attacttttt
     3781 gtaaatatca ttctatcttt cctttcccta tcagttatta tttattaaat tttatacgtt
     3841 tgatttttta ttttttatct gcttcttata ttgagcaatc tcttttttat tcattcttta
     3901 acagacaatt ttaatgtgta attttatcca ctctgtgata gtaactttaa tgaataatct
     3961 tatctactgt gatagttatt ttagtgagta attttatcta ctccacagca atcgctttaa
     4021 tgagtaatct tatccactct atgatagtta ttttagtgag taattttatc tactccacag
     4081 taatcgcttt aacgagtaat cttatctact ctataatagt cactttaatg agtaatcata
     4141 atttttatat cattttagat acttactcta ataagaattc attttctaat acttaataag
     4201 ttttaattac ttccacttta acctatatac taattaactt atttaattta gtgcatattt
     4261 gacactttac cttttattat accatttatt atcataaata taataattct atttttttaa
     4321 aataaatatt tataataatc taaaataaat atttataaat tctaatcaac cgtaaataat
     4381 aatctaaaat acatcaaaaa ataaactaaa aaatatcgaa a
//
LOCUS       DAYAPA010000200         4417 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.205, whole genome shotgun sequence.
ACCESSION   DAYAPA010000200 DAYAPA010000000
VERSION     DAYAPA010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4417
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.205"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            231..1310
                     /locus_tag="ACGI59_003660"
     CDS             231..1310
                     /locus_tag="ACGI59_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HFG9741409.1"
                     /translation="MPELLKNMYNRESLYEVAVAIQSVYNSFKVDEFIKSTMDETWNN
                     LELKARCRKISMSLGMYLPEDYKEALSILEKSVTGFYFAFFFPDFVEVYGQDDINWDL
                     SISALERNTEYWSSEFAVRAFIIKDEERMMAQMRKWSKHKSEHVRRLASEGCRPQLPW
                     GQAISKFKKDPTPVLPILEQLKTDTSTYVQKSVANNLNDISKTHPDLVISIVKDWYGK
                     NKSTNWIVKHGCRTLLKKGNRDVLALFGYDDTTSINLQDFTLETTSISIGEDLTFSFN
                     ILAKKATKTRLEYGIDYIKSNGKRNRKIFKISEVSLKENEKKSYMKKHSFADVSVRKH
                     YPGIHSIAIIINGIEKDKLDFELGI"
     gene            1634..2002
                     /locus_tag="ACGI59_003661"
     CDS             1634..2002
                     /locus_tag="ACGI59_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HFG9741410.1"
                     /translation="MNVYSFWNDTLAQDAVKIRKYFHEDAYINWHDTNEHFTLEEFII
                     ANCEYPDKWNGKVERVLEFGNLVITVTHVYAINKSLSFHVVSFISLKDDKIISIDEYW
                     GEDDDVPQWRLDKHIGKPIK"
     gene            2119..2922
                     /locus_tag="ACGI59_003662"
     CDS             2119..2922
                     /locus_tag="ACGI59_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFG9741411.1"
                     /translation="MEEKYILSDRGKVYYWIKESNSVFAKNLIFLPGLTANHHLFDFQ
                     IQYFSKNHNVLVWDAPAHGKSRPYLDFSYSNLVEELKVILDRQNMKKVVLIGQSAGGF
                     VAQSFIVKYPNMVEGLMMIGTCPYGTDYYSKSDIFWLKQTEWMLKLFPDKLLRNIISK
                     MCGATSEGRRSMLKMLEGYEKKELCHLMYMGFAGFIPEIQDLNIHCPVCLVVGDKDKT
                     GKVRKYNEQWHNKTGYPLHIIKNASHNVNVDKPNELNQIMDIFIKQLNI"
     gene            3314..4204
                     /locus_tag="ACGI59_003663"
     CDS             3314..4204
                     /locus_tag="ACGI59_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFG9741412.1"
                     /translation="MIESIPAKQILQKVKFDNTRWFGIDYNMNLYRGCSHGCIYCDSR
                     STIYNIENFDKVRYKENVIEILSKELRSKRKKGVVGIGAMSDTYNPFEKQLCITKQAL
                     DLISENHFGVSIDTKSSLVVRDIPILQKIKKNNSAIVKLTITTANDELSKKIEPYVNP
                     SSVRFDAVKELNDSGIFCGILLTPMLPFLTDNKDEIRAIVEKAHKANAKFIYCMYGVT
                     MRSGQREFFYEHLRDISPKLVLKYQKTYGLNYVCTVQNKDSCEKLLREECARYGILTD
                     MKDIIKAYKRSESYTQIKFF"
BASE COUNT         1682 a          485 c          832 g         1418 t
ORIGIN      
        1 cagtaaagta taaaattcta ttactataat aaaagataga actagaagtc tttatgaaag
       61 tgggaaaagt atttatataa ataaattcat atagtttata gtttaatagt aggaaatatc
      121 gtatgagtaa gaatattgta ttttacataa agatttagca atatgacaca ctgttgtcgt
      181 gttctgtagg atatgattaa taaatcagat atgaaaggag agtgagtaat atgccagaat
      241 tactaaaaaa tatgtataat cgtgagtcac tttatgaagt tgcagtagct atccaatcag
      301 tttacaactc atttaaggta gacgagttca taaaatctac aatggatgag acatggaata
      361 acttagaatt aaaagccaga tgtcgaaaga taagcatgag tttaggaatg tatttaccag
      421 aagactataa ggaagctctt tccattcttg aaaaatcagt tacaggtttt tattttgctt
      481 ttttctttcc agattttgtt gaagtttatg ggcaggacga cataaattgg gatttatcta
      541 taagtgcatt agaaagaaat actgagtatt ggtcttcaga atttgctgta agagccttta
      601 tcatcaagga cgaagagcgt atgatggcac agatgcgtaa atggtctaag cataagagcg
      661 aacatgtacg aagacttgca agtgaaggct gtcgtcccca gttgccttgg ggacaagcaa
      721 tatccaaatt taaaaaagac cctacacccg tactgcctat attggagcaa cttaaaacag
      781 atacatcaac gtatgtacaa aagagtgttg ctaataatct aaatgatatc tcaaaaacac
      841 atccagattt agtcataagc attgtaaagg attggtacgg taaaaataaa tccactaact
      901 ggattgtaaa gcatggttgc agaactctac taaaaaaagg caatcgtgat gtattagccc
      961 tttttggata tgatgatact acttctataa atcttcagga ctttacttta gaaacaacgt
     1021 ctatttctat tggagaggat ttaacttttt catttaatat tttagcaaaa aaagcgacaa
     1081 aaacaaggct cgagtacgga attgattata ttaaatctaa tggaaaaagg aataggaaga
     1141 tatttaaaat atcggaggtt tcattaaaag aaaatgagaa aaagtcatat atgaaaaaac
     1201 attcatttgc agatgtaagt gtaaggaaac attatccagg tatacactcg attgctatta
     1261 ttattaatgg tatagaaaaa gataaacttg attttgaatt aggtatataa ttttactaac
     1321 agaataatct atatcagata ctttgctaaa cttatataat atattttact aaatttatat
     1381 taggtagttt actacttgtt tactgaattt aaatacaaag aaatacaggt gctaatccct
     1441 gcccattaaa ggaatatgag cataacacaa aagtagaact tatcaattct gttacgggta
     1501 tagtagtaaa tccatagaat cacaattaga agatgaagtg taaaaaataa gttagtgttg
     1561 attttagttg ctagtgatat tgaatagtta tacaatagcc atataaatta gaatttgtag
     1621 aggagaaata taaatgaatg tatatagttt ttggaatgac actttggcac aagatgctgt
     1681 taagataaga aaatattttc atgaagatgc ttatattaat tggcatgata caaatgagca
     1741 ttttacttta gaagaattta ttattgcaaa ttgtgagtat cctgataaat ggaatggtaa
     1801 agtagaaagg gtgctagaat ttggaaattt agtaattaca gtgacacatg tttatgcaat
     1861 aaataaatca ctatcatttc atgtcgtttc atttatcagc cttaaagatg ataagattat
     1921 ttctattgat gaatattggg gagaggatga tgatgtgcca cagtggagat tagataaaca
     1981 tattggaaag ccaataaaat agtgtttgtt ctgttttata actgaaattt tgataacatt
     2041 tgatattttg atttgtcatt ggtcaacata gtcgttagtg taataattta aaaaagtaga
     2101 atttggagag tgaacactat ggaagaaaaa tacatcttat ctgaccgagg gaaagtatat
     2161 tattggataa aagaaagtaa tagtgtgttt gcaaagaact taattttttt gccaggacta
     2221 acagcaaatc atcatctttt tgatttccag atacagtact tttcaaaaaa tcataatgta
     2281 ttggtatggg acgctcctgc tcatggaaaa tcaagacctt acttagattt ctcatattct
     2341 aacttggtag aagaattgaa agtaatttta gatagacaaa atatgaaaaa ggttgtactt
     2401 atagggcaat cggcaggggg atttgtggca cagtccttta ttgtaaaata tccaaatatg
     2461 gtagaaggac taatgatgat tggaacttgt ccatatggaa ctgactatta tagtaagtca
     2521 gatattttct ggttaaaaca gactgaatgg atgttaaagt tattcccaga taaattgttg
     2581 cgaaacataa tatctaagat gtgtggagct acgtctgaag gcagaaggag tatgctgaaa
     2641 atgttagaag gttacgaaaa aaaagaactg tgtcatctca tgtatatggg atttgctggg
     2701 ttcataccag aaattcaaga cttaaatata cattgtccag tttgtttggt ggttggagat
     2761 aaagacaaga cgggtaaagt gagaaaatat aatgagcagt ggcacaataa gactgggtat
     2821 ccattgcaca ttataaaaaa tgcttcacat aatgtaaatg tcgataaacc taatgaacta
     2881 aatcagatta tggatatatt tattaagcaa ttaaatatat aaaaactaca tactcattag
     2941 gtaaattatg tagtatatgt gtttacagat ggttatagaa ataaatatgt aataaactac
     3001 ttaacactta aaaagtggat ttgtccagag cataatacaa agcatgatat atataaattt
     3061 ttaagcgaaa tacatcaaaa gaaggattag aattagtgga gacagaacag ctattataga
     3121 ttataaataa atagttttaa aggagaggta ctacgaatat agaggaaggt aaatcatacg
     3181 ttattcaagt attataaatg attagtttaa aagtactatc ggataggagt ctccatattt
     3241 aaaccttagt aaaggtagtt aaagtagtag tttaaaaact aaaaaattat ataaaggaat
     3301 taggtataag aaaatgattg agagtatacc agcaaaacaa attttacaaa aagtgaaatt
     3361 tgacaataca agatggtttg gaattgacta taatatgaat ctctatcgtg gttgttctca
     3421 tggatgtata tattgtgata gccgtagcac aatatacaac atagagaatt ttgacaaagt
     3481 tagatataaa gaaaatgtga tagaaatttt aagtaaagaa ttgcgttcaa aaagaaaaaa
     3541 aggtgtagta ggcattggag ctatgtctga cacatataat ccttttgaaa agcaactttg
     3601 tattacaaag caagcactag atttgatttc ggaaaatcat tttggagtgt ctattgatac
     3661 taaaagtagt ttggtggtta gagatatacc gattttgcag aaaattaaaa aaaataattc
     3721 tgctattgta aagttgacta taaccactgc aaatgatgaa ttatcaaaga aaatagaacc
     3781 ttatgtaaat ccaagttctg tacgatttga cgcagtgaaa gaacttaatg attctggaat
     3841 tttttgtggc atattattga ctcctatgct tccatttttg actgataata aagatgaaat
     3901 acgagcaatt gtagaaaaag cacacaaggc taatgcaaaa tttatttatt gcatgtatgg
     3961 tgtaacaatg cgtagtggtc agagagagtt tttttatgag catttaagag atatatcacc
     4021 aaaattggta cttaaatatc agaaaacata tggtttaaac tatgtatgta ctgtacaaaa
     4081 taaagattct tgtgagaagt tgttaagaga agaatgtgca cgttatggta ttttgacgga
     4141 tatgaaagat attataaaag cgtacaagag gtcggagtca tatactcaaa taaaattttt
     4201 ttaagatagg ctttggatga gatataatag tgttgattga tgtgttgata ttatagttga
     4261 ttgttagaga ggatattata gatgaatatt agtattgata ttttaggtaa ttgttagagc
     4321 tgatattata gatgaatatt agtattgata ttctaggtaa ttgctagagt tagtattata
     4381 gatgaatatt agtattgata ctctaggtaa ttgctag
//
LOCUS       DAYAPA010000201         4124 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.206, whole genome shotgun sequence.
ACCESSION   DAYAPA010000201 DAYAPA010000000
VERSION     DAYAPA010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4124
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.206"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            69..1811
                     /locus_tag="ACGI59_003664"
     CDS             69..1811
                     /locus_tag="ACGI59_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloendopeptidase"
                     /protein_id="HFG9741413.1"
                     /translation="MVKSKKKIFVFLILIAVFIIFASYIRNYTNDTKKDSVRGTIPFE
                     KMEYKRPNIQSICENINKCNDKLLTAKTAKEQLNLFNEVDKIYQDFYSTLTIAKIRNN
                     IDSSDEFYSKEYKYLMSKSVDVDMSYKDFQDRFVTSKFSKELEKELGKDTFNYLKNIG
                     KLNSKEVEGLLKKEQDLVVKYEDLLAKSTVSIDGIEVDFEEALSRPNLSPEEYVKIYS
                     DYLKKYNPIFGNIFLELIQTRTEIASKQGFKNYIDYAYMNLNKDYSQEEAKKFRQDVK
                     DYIVPLYREISSKPSDSSIYIKVYKNRSFRKFDKVLEDISPKLKESFDYMKKYDLYDY
                     SSGKNKSPGGYTTYINKYKAPFLFNTWDNSFLGVTSFAHEFGHFYNYYNSINLNNKMQ
                     PSIDVCEVHSTSLEILFYKYFDDFFGKQSEAIKKEHLSIVLNTIIDACLYDEFQEIIY
                     KNPYMSLNDINKLFFDLEEEYGVSNNILREKNAPFWILVSHNFQVPFYYLSYGLASDV
                     SLQIWQLSQEDYRKAVDVYMDFLNQNTDAGFKDVVEKVNLQLPFQDGNLEEISSTLYD
                     YFGIDNPLELKNAS"
     gene            complement(1981..2169)
                     /locus_tag="ACGI59_003665"
     CDS             complement(1981..2169)
                     /locus_tag="ACGI59_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9741414.1"
                     /translation="MKIITIGSSLITVLLFLSTMICGFWIKNNKVTDASSIKFHMNSA
                     IFTGIFLLISTILLIIYI"
     gene            complement(2166..2747)
                     /locus_tag="ACGI59_003666"
     CDS             complement(2166..2747)
                     /locus_tag="ACGI59_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFG9741415.1"
                     /translation="MKSNDKDARERIIEVTLNLLNEVDDIEEITVRKIAERANVGVGL
                     INYHFKTKDNLLSTAIGDVMSNIIAELYDDSVYTLRPIEDLKNLLKKLCDTGLHYEKV
                     LPFVLNQCITNGDMQAELDIVPMLRKIFGNKKDEMSLRIIALQIILPIQISALSTESF
                     QLYSGINIKNKYERDKFIDILIENIIGEDVDVR"
     gene            2915..3733
                     /locus_tag="ACGI59_003667"
     CDS             2915..3733
                     /locus_tag="ACGI59_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFG9741416.1"
                     /translation="MFKIGDFSKLSKISIRMLRHYDEIGLLTPSHTNKTNGYRYYSAD
                     QLSTTNRIHALKDMGFGLYSIKEILTEYNDKESLIKYLNIHHSQVKEQLEDTQKKLLK
                     IETTIKRIGGNDIMKNYDVTIKNFAPKYMMTLRRVIPTYQDEGMLWHQAFLETKDQNV
                     QIEPPKYSKAVFYDTGYKEDYVDVEVQVAVSGKYKDTEHVKFKTVPSVTAATAIVNGN
                     FNQVADACEAIGNWISDNNYDVDGPMFNIYHVSPGNDSNPDNWVTEVCFPVKKK"
BASE COUNT         1568 a          524 c          561 g         1471 t
ORIGIN      
        1 taacatgcat tttttatatt taatatagta aaataattaa taactatatc catgattgga
       61 ggatttttat ggttaaatcc aagaagaaaa tatttgtttt tctaatttta attgcagttt
      121 ttattatatt tgcatcatac ataagaaatt atactaatga tacaaaaaaa gattctgtta
      181 gaggaactat tccttttgaa aaaatggaat ataagcgtcc taatatacag tcaatttgcg
      241 aaaacattaa caagtgcaac gacaaacttt taacagctaa aacagctaag gaacaattaa
      301 atttatttaa tgaagttgat aaaatttatc aagattttta cagtacactt actatagcaa
      361 aaattagaaa taacatagat tcatcagacg aattttactc taaggaatat aagtatctta
      421 tgagtaaatc agtagatgtt gacatgtcat acaaagactt tcaagataga tttgtaactt
      481 ctaaattctc taaagaatta gaaaaagaac ttggaaagga tacatttaat tatctaaaga
      541 acattggaaa acttaattct aaagaagttg aaggtttgct taaaaaagag caagacctag
      601 ttgtaaaata tgaagactta ttagctaaat ctacagtatc aatagatggt atagaggtag
      661 actttgaaga agccttgtca agaccaaatc ttagccctga agaatatgtg aaaatttatt
      721 cagattactt aaaaaaatac aatcctatat ttggtaacat attcttagaa cttatacaga
      781 caagaactga aatagcaagt aagcaaggat ttaagaatta tatagattat gcctatatga
      841 acctaaacaa agactattct caagaagaag ctaaaaaatt tagacaagat gtaaaagatt
      901 acatagttcc attatataga gagatttcat ctaaaccatc tgattcttct atttatatta
      961 aagtatataa aaatcgctca tttaggaagt ttgataaagt acttgaagat atatctccaa
     1021 aactaaaaga gagcttcgac tatatgaaaa aatatgactt gtacgattat tcttctggaa
     1081 aaaacaaatc acctggagga tatactactt atataaataa atataaagca cctttcttgt
     1141 ttaatacatg ggataattct tttttaggtg ttactagttt tgctcatgag tttggtcatt
     1201 tttataatta ctacaacagt attaacttaa acaataagat gcaaccaagt atagatgtat
     1261 gtgaagttca ttcaacatct ttagaaattc tattttataa atattttgat gatttttttg
     1321 gtaaacaatc tgaagctata aaaaaagaac acttatcgat tgttttaaat actattattg
     1381 atgcttgtct ttatgatgaa tttcaagaaa ttatatacaa gaatccatat atgagtctaa
     1441 atgatatcaa taaattgttt tttgatttag aggaagaata tggagtttcc aataatatac
     1501 ttagagaaaa aaatgctcct ttttggatac ttgtatctca taacttccaa gttccttttt
     1561 attacttgag ttatggtctt gcatctgatg tatcacttca gatatggcaa ttatcacaag
     1621 aagattacag aaaagctgtt gatgtatata tggatttttt aaatcaaaat actgatgctg
     1681 gatttaaaga tgtggtagaa aaggtaaatt tacaattacc tttccaagat ggaaatttag
     1741 aggaaataag ctctacttta tatgattatt ttggtataga taatccgctt gaactaaaaa
     1801 atgcttctta atttctaata ctttatactc actcttaaga tttagtatta gattgaaata
     1861 agtgatattt tttcacacac aattaatgtt taataaaatt atatggtaaa gtatcagaaa
     1921 tttttaagac tcttgtcttt ataatttctg atactttatt tattaataat attattattt
     1981 ttatatatat ataattaaaa ggattgtgga aatcaataaa aatatccctg taaaaatagc
     2041 actattcata tggaacttaa tagaacttgc atctgttacc ttattattct taatccagaa
     2101 cccacaaatc atagttgaaa gaaaaagtaa aacagtgata agacttgaac caatagttat
     2161 aattttcatc ttacatcaac atcctctcca ataatatttt ctattaaaat atcaatgaat
     2221 ttatctcgct catatttatt ctttatattt ataccactgt ataactgaaa tgattctgta
     2281 gataaagcag aaatctgtat tggtaaaata atttgcaggg caataattct tagactcatt
     2341 tcatcttttt tatttccaaa aattttacga agcataggga caatatcaag ttctgcttgc
     2401 atatctccat tggttataca ttgattcaat acaaaaggaa gcactttttc ataatgtaat
     2461 ccagtatcac acaatttttt taataaattt tttaaatctt caataggtct tagagtatat
     2521 acactatcgt catatagctc tgctattata tttgacatca catctcctat ggcagtactc
     2581 aacaaattat cctttgtttt aaaatgataa tttattaaac caactccaac atttgcacgc
     2641 tcagcaattt ttctaactgt aatctcctca atatcatcta cttcatttaa aagattcaat
     2701 gtaacttcaa ttatacgttc tctagcatct ttatcattac ttttcataat agacctccaa
     2761 aacaattaac attttcagtt ctgaacatgt tcagtataat atttttttca tcatatgtca
     2821 atataattta attataattt tagttgactc taacattatg atatggttta tattaatatt
     2881 aagctaaaag tacctttata aaggagacaa aaacatgttt aagataggcg atttttcaaa
     2941 gctaagtaaa attagtattc gtatgttacg tcattatgat gaaataggac ttttaactcc
     3001 ttctcatacg aataaaacta atggatatag atactatagt gcagaccaac tttctactac
     3061 aaacagaatc catgcactaa aagatatggg ctttggcttg tattctataa aagaaatttt
     3121 gacagaatat aacgataaag aaagtctcat aaaatatcta aatatccacc attctcaagt
     3181 aaaagaacaa ttagaagata cacagaaaaa actattaaaa atagaaacca ctattaaaag
     3241 aattggagga aatgatatta tgaaaaatta tgatgtaact ataaagaatt ttgcaccaaa
     3301 atatatgatg acacttagaa gggttatacc aacttatcaa gacgaaggaa tgttatggca
     3361 tcaagcattt ttagagacaa aagaccagaa tgttcaaatt gaacccccaa agtattctaa
     3421 agcagttttt tatgatactg gatataaaga agattatgta gatgttgaag tacaagttgc
     3481 tgtatctgga aagtataaag atactgaaca tgttaaattt aagactgttc caagtgtgac
     3541 tgctgcaact gcaattgtta atggaaattt caatcaagtt gcagacgctt gtgaggctat
     3601 tggtaattgg atatcagata ataactatga tgtggatggt cctatgttta atatttatca
     3661 tgtaagtcct ggcaacgata gtaatcctga taattgggta acagaggtat gcttcccagt
     3721 taaaaagaag taatttttat attaattgga taatatttta atttgttaga caaattactt
     3781 ttagtgttat gtataaatac ctacaattta attaggatat tatcttaatt tgttaaacaa
     3841 attactttta gtgttatgta taaatatcta caatttaatt aggatattat cttaatttgt
     3901 cagataaatc acttgtgatg ttatgtttga atgactagaa tttaatcagg atattatctt
     3961 aatttgtcag ataaatcact tgtgatgtta tgtttaaatg actagaattt gattgggata
     4021 ttatcttaat ttgtcagata aatcaattgt gatgttgtgt ttaaatgact agaatttgat
     4081 taggatatta tcttaatttg tcagataaat tatttataat gtta
//
LOCUS       DAYAPA010000202         4110 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.207, whole genome shotgun sequence.
ACCESSION   DAYAPA010000202 DAYAPA010000000
VERSION     DAYAPA010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4110
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.207"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            <1..2389
                     /gene="priA"
                     /locus_tag="ACGI59_003668"
     CDS             <1..2389
                     /gene="priA"
                     /locus_tag="ACGI59_003668"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFG9741417.1"
                     /translation="GHRILVPFGKGNKPTEAFVFQFTDSLDEKIKIKEIIDILDENPI
                     FRKEDLELVYWMKNRYLCTYIECINLIYPKGYKLNNYKVVLLGESLSGLNDIELKEKI
                     STLSDKNREIVEKVIDSKGKIKVDKLKYIPNLNSSLYTMNKNGIIKLCWEYKNHKNEK
                     KVCYISLSLESDKIDDYIEQNKINVGSKQKEILSFLKNNENVEINDLLDLLNASKQSI
                     NSLSKKKLITLEFKDYYREPKSIYKSVLKSIKLNNEQQEAVDEIKSNMFVDDKKPYLI
                     HGVTGSGKTEVYMEIIEFALNQGLDSIFLVPEIALTPQTIDRLKSRFGDLVGVFHSKL
                     SEGEKHDVYKAVKAGKVRVLIGARSALFAPFNSLGLIIIDECHESSYKSEKNPKFNAI
                     EVARFMALKNNITLILGSATPSIEEYYRAKSGEYKLINIKSRANDKPLPNIEVVDMKD
                     ELDKGNRSIFSLKLQKEIRYAIEENNQVILFLNRRGYANFVSCRKCGYVFQCENCDIS
                     LTYHKKSNTGRCHYCGYEKEIPKECPECKSTYVKPFGVGTQKIEEELKYIFPDIKTLR
                     MDKDTTSKKGALDEILNKFKDKEADVLIGTQMLSKGLDFENVTLVGILSADMILNFPD
                     FKSFETTFQLITQVSGRAGRADKEGKVVLQTYDTEHYAIKHAIEYDYEGFYEDEIKIR
                     KAFGYSPFNNMLSVVVSGEDERLVIKNIKNMHASLIYLLEKRGINDLGFILGPNPCSI
                     SKINQNYRWQILFKDENIEINLLKGIIKYICITKRDLIFDKNINVSIDINPNSVL"
     gene            2407..2847
                     /gene="def"
                     /locus_tag="ACGI59_003669"
     CDS             2407..2847
                     /gene="def"
                     /locus_tag="ACGI59_003669"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFG9741418.1"
                     /translation="MALRQIVQIGEPVLRKKSKKVEKIDEKIIQLLDDMAETMYDADG
                     VGLAAPQVGILKRVVVIDIGEELIELINPEIIETSGEQIDEEGCLSVVGEAGNVRRPN
                     YVKVRALNRNGETIELEGEELLARAFCHEIDHLDGILFVDKIEK"
     gene            2862..3791
                     /gene="fmt"
                     /locus_tag="ACGI59_003670"
     CDS             2862..3791
                     /gene="fmt"
                     /locus_tag="ACGI59_003670"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFG9741419.1"
                     /translation="MKIVFMGTPDIAVPCLQKIIDEKYEILGVVTQPDKPKGRGKKLG
                     MSPVKELAIENNIPVYQPVKARDKEFIDKIKSLNPDVIVVVAFGQILPKEILEIPKLG
                     CINVHVSLLPKYRGAAPINWVIINGEEKTGVTTMYMDEGLDTGDMILKTEVNLDENIT
                     AGELHDKMMNIGAETLKETLRLIEEGNAPREVQNHEEFSYAPIMNKSLGNIDFSKSAR
                     EIHNLVRGVNPWPSAYTTYNDVIMKVWKTKVLDEKSTKDVGTIIDVSKDGIKVSTIDN
                     VLLIEEIQMPNKKRMLVGEYIKGNTIETGLVLG"
     gene            3801..>4110
                     /locus_tag="ACGI59_003671"
     CDS             3801..>4110
                     /locus_tag="ACGI59_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF116 domain-containing protein"
                     /protein_id="HFG9741420.1"
                     /translation="MTDIKKYLTSVGILILILGILIGVVNFFIISLTQIFSLMINIII
                     ISLILIILCSTAVTYRVIKGKYVNSNIMKINFNIVSGLFPIISFIASSFGMSKSDIRR
                     I"
BASE COUNT         1718 a          380 c          776 g         1236 t
ORIGIN      
        1 tgggcacaga atattagtac catttggaaa aggtaataaa ccaacagaag cctttgtatt
       61 tcaatttaca gatagcttag atgaaaaaat aaaaataaaa gaaattattg atatattaga
      121 tgaaaatccg atatttagaa aagaagattt agagttggtt tattggatga aaaatagata
      181 tttgtgtact tatatagaat gtataaattt gatatatcca aagggataca aattaaataa
      241 ttataaagta gtcctattag gtgaaagttt aagtggatta aatgatattg aattgaagga
      301 aaaaatatca actttaagtg ataaaaatag agaaatagta gagaaagtga ttgatagtaa
      361 aggtaaaatt aaggttgata agttaaaata tataccaaac ttaaatagtt ctttgtacac
      421 aatgaataag aatggaatta taaaattatg ttgggaatat aaaaatcaca aaaatgaaaa
      481 gaaggtatgt tatatatctt tgagtttaga aagtgataag attgacgatt atatagagca
      541 aaataaaata aatgtgggaa gtaaacaaaa agagatatta agttttctta aaaataatga
      601 aaatgtagag ataaatgatt tattggattt attaaatgct tcaaaacaaa gtataaattc
      661 tctaagtaag aaaaagctaa taaccctaga atttaaggat tattatagag agccaaaaag
      721 tatttacaaa tccgtactaa aaagtataaa attaaacaat gagcaacaag aagcagttga
      781 tgagataaag tcgaatatgt ttgtagatga taaaaaacca tacttaattc atggagtaac
      841 tggaagtgga aaaactgaag tttacatgga aataatagaa tttgctctaa atcaaggctt
      901 agacagtata ttcttagtac ctgagatagc attaactcct caaacaatag ataggctaaa
      961 gagtaggttt ggagatttgg ttggagtgtt ccacagtaaa ctatctgaag gagaaaaaca
     1021 tgatgtttac aaagctgtca aagcaggtaa agtaagagtg ttaattggag ctaggtcagc
     1081 tttatttgca ccttttaatt ccttaggctt aataataatt gatgaatgtc atgagtcatc
     1141 atataagtct gagaagaatc caaaatttaa tgctatagaa gttgctagat ttatggcatt
     1201 aaaaaataat ataactctta ttttaggttc agcaacgccc tcaattgaag agtattatag
     1261 ggcaaaatct ggagaataca aacttataaa tataaaaagt agagcaaatg ataagcctct
     1321 tccaaacatt gaagtggtag acatgaaaga tgagttagat aaaggaaata gaagtatatt
     1381 tagtttgaaa ttacaaaaag aaatacgtta tgcgattgaa gaaaataatc aagtaatact
     1441 atttttaaac agaagaggtt atgcaaattt tgtatcttgt agaaagtgtg gatatgtgtt
     1501 tcaatgtgaa aattgtgata tatctttaac ttatcataag aaaagcaata ctggaagatg
     1561 ccactattgt ggatatgaga aagaaattcc aaaagagtgt cctgaatgta aaagtactta
     1621 tgttaagcca tttggagttg gaactcaaaa aattgaagag gaattaaaat atatttttcc
     1681 agatataaaa actttgagaa tggataaaga tacaacttca aaaaaaggtg ctttagatga
     1741 gatactaaat aaatttaaag ataaagaagc agatgtactt attggaacac agatgttaag
     1801 taaagggctg gattttgaga atgtaacttt agtaggtatt ttgtctgcag acatgatatt
     1861 aaattttcca gactttaaaa gttttgaaac tacatttcaa ttgataactc aagtctctgg
     1921 aagagcaggg agagctgata aagagggaaa agtagtactt cagacttatg acactgaaca
     1981 ctatgctatt aagcatgcta ttgaatatga ttatgagggt ttttatgaag atgagataaa
     2041 aataagaaaa gcatttggat attctccttt taataatatg ttaagtgtag ttgttagtgg
     2101 tgaagatgaa agactagtga taaaaaatat aaaaaatatg catgcatcat tgatttattt
     2161 attagaaaaa aggggtataa atgatttagg atttatatta ggtccaaatc catgttcaat
     2221 atcaaaaata aatcaaaatt atagatggca aatactcttt aaagatgaaa atattgaaat
     2281 taatctctta aagggtataa taaaatatat atgtataaca aaacgagatt tgatatttga
     2341 taagaatatc aatgtatcta tagatattaa tccaaacagt gtattataaa tttaggagga
     2401 attataatgg ctttaagaca aatagttcaa ataggagaac cagtattaag aaaaaaatca
     2461 aaaaaggttg aaaaaattga tgaaaagata atacaattat tagatgatat ggcagaaact
     2521 atgtatgatg cagatggtgt tggactggct gcacctcaag ttggaatatt aaaaagagta
     2581 gtagtaatag atattggaga agagcttata gaacttataa atcctgaaat aatagaaaca
     2641 tctggggagc aaatagatga ggaaggatgt ttaagtgttg ttggagaagc gggaaatgtt
     2701 agaagaccaa attatgtaaa ggtcagagct ttaaatagaa atggtgaaac aatagagtta
     2761 gagggagaag aacttttagc tagagcattt tgtcatgaga tagaccactt agatggaatt
     2821 ttgtttgttg acaaaataga aaagtagtag gagttgattt gatgaaaata gtatttatgg
     2881 gaactcctga tatagcagtt ccatgtttac aaaaaataat agatgagaag tatgaaatat
     2941 tgggtgttgt aactcaacca gataaaccaa aaggaagagg taaaaaacta ggtatgagtc
     3001 cagtaaaaga actggcaatt gaaaataata tacctgtata tcaaccagta aaggctagag
     3061 ataaagaatt tatagataaa attaaatctt taaatccaga tgtaatagta gttgtagctt
     3121 ttggacagat acttccaaaa gaaatattag agattcctaa acttggatgt ataaatgttc
     3181 atgtttcttt acttccaaaa tatagaggtg cggcacctat aaattgggta ataataaatg
     3241 gtgaagaaaa gactggtgtt acaactatgt atatggatga agggctagat actggagata
     3301 tgatactaaa gacagaagta aatcttgatg aaaatataac agcaggagaa cttcatgata
     3361 aaatgatgaa tataggcgca gaaactttaa aagaaacatt aagattaatt gaagaaggaa
     3421 atgctccaag agaagtacaa aatcatgaag aattttccta tgcgccaata atgaataaat
     3481 cattaggtaa catagatttt tcaaaaagtg ccagagaaat acacaattta gttagaggag
     3541 taaatccatg gccaagtgcc tatactacct ataatgatgt cataatgaaa gtttggaaaa
     3601 ctaaagtgtt agatgaaaaa agcacaaaag atgtaggtac aataattgat gtaagcaaag
     3661 atgggataaa agtaagtact atagataatg tacttttaat tgaagaaatt cagatgccga
     3721 ataaaaagag aatgttggtt ggtgaatata taaaagggaa tactatagaa actggtttag
     3781 tattgggtta ggtgaaaagt atgacagata taaagaagta tttaacaagt gttgggattt
     3841 tgattttaat attaggaatt ttgataggag ttgttaattt ttttataata agtttaactc
     3901 aaatattctc cttgatgata aatattataa taatatcact aatacttata atactatgtt
     3961 caacagcagt tacttataga gtaattaaag gaaagtatgt taactcaaat ataatgaaaa
     4021 taaattttaa tattgtcagt ggattatttc ctattatatc tttcattgca tcatcatttg
     4081 gtatgtcaaa aagtgatatt agaagaatat
//
LOCUS       DAYAPA010000203         4097 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.208, whole genome shotgun sequence.
ACCESSION   DAYAPA010000203 DAYAPA010000000
VERSION     DAYAPA010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4097
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.208"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            314..1789
                     /locus_tag="ACGI59_003672"
     CDS             314..1789
                     /locus_tag="ACGI59_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HFG9741421.1"
                     /translation="MDSEFANAVELSYECAPLLESLKEYSEKDIACFDVPGHVKNRGV
                     AILNKYLGESLMKMDINSSPTMDNVSAPNGIIKNAQDLLAQAYMADEAFFITNGTTQA
                     IHAMILSVIKPGEKVLLPRNIHKSVINALILCGGIPIFIQPEFDEKLGISLNITLEKV
                     RTEIEKDCNIKALFFLNPTYYGVCADLESIIELCHKNNVLVLVDEAHGAHFPFHLDLP
                     PSAISLGADMVAVSIHKTGGALTQSSALLLNRDNVSFEKVLQSINMLQSTSASYLLMA
                     SIDGARVNLVENGEKQLSKALNLSRYAKSKLNKIDGIKVLSTEILKQKGVKFIDETKL
                     CINVKELNLTGFEVYDLLYKNFSIQVELGDSYNILALVSIGTNKSDIDRLVKALSIIA
                     KIYRKESTLNEFNMVQINPIIKLNPREAFYAPKESVEINSCIDRICGESIMAYPPGIP
                     IIAPGELITEEIMEYIIFLKNSNAYLTDVQDKNLDRILVIK"
     gene            1876..2289
                     /gene="speD"
                     /locus_tag="ACGI59_003673"
     CDS             1876..2289
                     /gene="speD"
                     /locus_tag="ACGI59_003673"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HFG9741422.1"
                     /translation="MKFETLGRHILVEYYNCDENVLKDPRLIENFMNDSALNAKATIV
                     DSVFHHFNPWGVSGAVIIAESHLTIHTWPEYGYAAADFFTCGDIDPWKSFELLEQLLK
                     SERSESTEIPRGLTTKIKKYSKKDLGKITHKPEAV"
     gene            2319..3170
                     /gene="speE"
                     /locus_tag="ACGI59_003674"
     CDS             2319..3170
                     /gene="speE"
                     /locus_tag="ACGI59_003674"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HFG9741423.1"
                     /translation="MELWYTEEWTENVRFSIKVNKHLFEGKSQFQRIDVFDSDEFGKF
                     LTIDGLMMVTYKDEFIYHEMITHVPMATNLNIKKVLVIGGGDGGTVRELSRYPQIEKI
                     DMVEIDKMVVDVSKEYMDICSCKLDDKRVSLYFEDGVNFVKCAHDKSYDLIIVDSTDP
                     IGPGEGLFSTDFYKDCYRILTDDGILVNQSESPYFDFNAKEMKRANKKLKQIFPISEV
                     YQAHIPTYPSGHWLFGFASKKLNPVKNQDRNGWEKLSLKTKYYNSDIHLGSFMLPQYV
                     KEMLDEE"
     gene            3160..4038
                     /gene="speB"
                     /locus_tag="ACGI59_003675"
     CDS             3160..4038
                     /gene="speB"
                     /locus_tag="ACGI59_003675"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HFG9741424.1"
                     /translation="MKNNFYHMNTFMSMDKNYEESNLIVFGVGFDGTTSNRPGARFAS
                     SSMRKEFYGLETYSPFLDLDLEDYNICDYGDLEISVGSTEQVLKEIYQETYKIVRDSK
                     VPFMIGGEHLVTLPAFKAVHEKYNDIYVIHFDAHTDLREEYNNSKNSHATVIKRIWDI
                     VGDNKIFQFGIRSGTKEEFKFATEEKHTYMEIGGIDTFENIVNMLNGKNIYLTIDLDV
                     LDASVFPGTGTPEPGGVNYREFQEIFKIIKNSNINIVGCDIVELSPDYDTTGVSTVIA
                     CKILRELCLIISDKIK"
BASE COUNT         1655 a          435 c          726 g         1281 t
ORIGIN      
        1 attaaaaata gtaaactcaa aagcttacta aaactcaaaa aagtaaaaaa aacaaaataa
       61 taaaaaataa gaaagtttat aaatactaag ttttaaaaaa attaatataa acaacaaaaa
      121 aataaaaagt ttataaatat tgatatagct aatgtatatg tttttgagta acttcaaaaa
      181 aacacttgta aaaatgctga gatgttaata taataaatat catgagttta ataaaagtaa
      241 aaataaaaaa tagaaataat aaataataaa tattaaaaat atttgaatat agagaggaga
      301 tgaatttgaa atcatggata gcgagtttgc aaatgcagtt gaattaagct atgagtgtgc
      361 tccattatta gagtcgttaa aggaatactc agagaaagat atagcttgtt ttgatgtgcc
      421 tggacatgta aaaaatagag gtgtagctat attaaataaa tatctaggtg aaagtctcat
      481 gaaaatggat ataaattcat caccaactat ggataatgta tctgcgccaa atgggattat
      541 aaaaaatgca caagatttgc tagcacaagc atatatggct gatgaggcat tctttataac
      601 gaatggcaca actcaagcaa tacatgcaat gattttgagt gttataaaac caggtgaaaa
      661 agtattactt ccaagaaata tacataaatc agtaataaat gcgttaatac tttgtggagg
      721 aataccaata tttatacaac cagaatttga tgaaaaacta ggtataagtt taaatattac
      781 tcttgaaaaa gttagaacag agatagaaaa agattgtaat ataaaagcat tatttttcct
      841 taatccaact tactatggag tttgtgcaga ccttgagagt attatagaac tatgccataa
      901 aaataatgtt ttagtgttag ttgatgaagc tcatggtgca cattttccat ttcatttaga
      961 tttacctcct tctgcaataa gtttgggagc tgatatggta gctgtaagta tacataaaac
     1021 tggaggagca ttgacacaat cttcagcact cttattgaat agagataatg taagttttga
     1081 aaaggttctt caatcaatca atatgctaca atcaacatca gcatcttatt tgcttatggc
     1141 aagtatagat ggtgctagag ttaatctagt tgagaatggt gaaaagcaac tgtcaaaagc
     1201 attaaatcta tctaggtatg caaaatctaa attaaacaaa attgatggaa taaaagtttt
     1261 atctacagaa atattgaaac aaaaaggtgt taaatttata gatgaaacaa aattgtgtat
     1321 aaatgtaaaa gaattaaatt tgactggatt tgaagtatac gatttattat ataaaaattt
     1381 ttcaattcaa gtagaattgg gagattcata taatatactg gcacttgtgt ccattggaac
     1441 aaataaatca gatatagata gattagtaaa agcactttct ataattgcaa agatttatag
     1501 aaaagaatca acattaaatg aatttaatat ggttcaaata aatccaatta ttaaacttaa
     1561 tccaagagaa gctttttatg caccaaagga aagtgtagaa atcaattcat gtatagatag
     1621 aatttgtgga gaatcaataa tggcatatcc accaggtata ccgattattg ctccaggaga
     1681 attgataaca gaagaaatta tggagtatat tattttctta aaaaatagca atgcatatct
     1741 tacagatgta caagataaaa acttagatag aatattagta ataaaataaa ttactttatt
     1801 ttgcaagggc aagtatttgt taagtagagt ttatcaaata gaacttatta aaagtataaa
     1861 ggagttgggg ttgaaatgaa atttgaaaca ctaggaagac atatactagt agaatactat
     1921 aattgtgatg aaaacgtact taaagaccca cgtcttatag aaaattttat gaatgattca
     1981 gcattaaatg caaaagcgac aattgtggat tctgtattcc accattttaa tccttggggg
     2041 gtttcaggag ctgttataat tgctgaatct catttgacaa tacatacatg gcctgaatat
     2101 ggctatgcag cagcagattt ttttacttgt ggtgatatag acccatggaa aagttttgaa
     2161 ttattagagc aacttttaaa gtctgaaaga agcgagtcta cagaaattcc aagaggctta
     2221 actacaaaaa ttaaaaaata ttcaaaaaaa gatttaggta agattactca taaaccagaa
     2281 gcagtataaa acatacaaga gaaagtgagg attattatat ggaactttgg tatacagaag
     2341 aatggacaga aaatgtaaga ttttctataa aagtaaataa acatctattt gaaggaaagt
     2401 ctcaatttca aaggatagat gtgtttgata gtgatgaatt tggaaaattt ctaacaatag
     2461 atggattaat gatggtaaca tataaagatg aatttatata ccatgagatg ataactcatg
     2521 ttcctatggc tacaaattta aacataaaga aagtattagt cataggtgga ggtgatggag
     2581 gtacagtaag agaattaagc cgttatcctc aaatagaaaa gattgacatg gttgaaatag
     2641 ataaaatggt tgtagatgta tctaaggagt atatggatat atgctcatgt aaattagatg
     2701 ataaaagagt gagtttatat tttgaagatg gtgttaattt tgttaagtgt gcacatgata
     2761 aatcatatga cctgataata gtagattcaa ctgacccaat aggaccaggt gaagggcttt
     2821 tctcaactga tttttataag gattgttata gaatactgac tgatgatgga attttagtaa
     2881 atcaaagtga aagtccgtat tttgacttta atgctaaaga gatgaaaaga gctaataaaa
     2941 aattaaaaca gatatttcca atatcagaag tttatcaagc acatatacca acatatccat
     3001 ctggtcactg gttgtttgga tttgcttcaa aaaaacttaa tccagtaaaa aatcaagata
     3061 gaaatggatg ggagaaatta agtttaaaaa ctaaatatta taatagtgat atacatttag
     3121 gttcgtttat gttacctcaa tacgtgaagg agatgctaga tgaagaataa tttctatcat
     3181 atgaacactt ttatgagtat ggacaaaaat tatgaagaat ctaatcttat agtatttggt
     3241 gttggatttg atggaactac ttctaacaga cctggggcta gatttgcaag tagctctatg
     3301 agaaaagaat tttacggtct tgagacatat agtccttttt tagatttgga tttagaggat
     3361 tataatatat gtgattatgg agatttagaa attagtgttg gaagtacaga acaagtctta
     3421 aaagaaatct atcaagagac atataagatt gttagagatt caaaggtacc ctttatgatt
     3481 ggaggagagc atttagttac attaccagcc tttaaagcag tacatgaaaa gtacaatgat
     3541 atatatgtaa ttcattttga tgcccatact gatttgaggg aagaatataa taatagtaaa
     3601 aattctcatg caacagtaat taaaagaata tgggatattg taggtgataa taaaatattt
     3661 caatttggta taaggtctgg gacaaaagaa gaatttaaat ttgctacaga agaaaaacac
     3721 acatacatgg aaataggagg aatagatacg tttgaaaata tagttaatat gctaaatgga
     3781 aagaatattt atctaactat agatttagat gtattggatg catctgtgtt tccaggaaca
     3841 ggtacaccag aacctggtgg tgtaaattat agagagtttc aagagatttt taagattata
     3901 aaaaactcta atataaatat agttggttgt gacattgtag agttaagccc tgattacgat
     3961 acaacaggtg tatcgacagt tatagcttgt aaaatcttaa gagagttatg cttaataata
     4021 tctgataaaa ttaaatagag tattttatat aaatagtata tattataata taagaagtta
     4081 ttttaaaaaa gttagtt
//
LOCUS       DAYAPA010000204         3988 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.209, whole genome shotgun sequence.
ACCESSION   DAYAPA010000204 DAYAPA010000000
VERSION     DAYAPA010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3988
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.209"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(235..615)
                     /locus_tag="ACGI59_003676"
     CDS             complement(235..615)
                     /locus_tag="ACGI59_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HFG9741425.1"
                     /translation="MCLKKLSKLELVIMKFIWNLDIKTNSYEIIDYMKEEHNLPEKVA
                     LKTLSKLSKKRFLYVQETGKCMYYTVAIKEKAYLEFISRNVQNLLKNNFIRNLLVSFH
                     EEELTEEKIKSLENWVVNWEEAYV"
     gene            complement(816..2009)
                     /locus_tag="ACGI59_003677"
     CDS             complement(816..2009)
                     /locus_tag="ACGI59_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFG9741426.1"
                     /translation="MLTSLALIFLLGMASGGIFKRIKLPNLLGMLLTGIILGPYVLNL
                     IDNSILDISSDLRKIALIIILTRAGLSLDINDLKKVGRPAVLMCFIPATFEIIGMIVL
                     APKLLGVSILEAAVMGAVVGAVSPAIIVPKMLKLMEEGYGTEKSIPQMLLAGTSIDDI
                     FVIVMFTVFTGLAQGNSISAISFLQIPVSIILGVIVGAVIGICLAVFFKKVHMRDSAK
                     GVLLLSISFLMISLETALEGIVPFSGLLAVMSIGIFLQIKYRVVARRLSIKYSKLWVG
                     AEILLFVLVGATVDISYAFKAGIGAVILIFGVLLFRMVGVFFCLIKTNLTIKERLFCM
                     IGYIPKATVQAAIGGVPLAMGMASGQLILTLAVLAILITAPLGAFGIDVTYKKLLTSV
                     KGESK"
     gene            complement(2072..3328)
                     /locus_tag="ACGI59_003678"
     CDS             complement(2072..3328)
                     /locus_tag="ACGI59_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HFG9741427.1"
                     /translation="MIEKFKTLIWIKMIIRIKSVYKTYGGLFFLGLIGIPVGAIIGLI
                     DTIFGTVLLKVTDIRETYPMYLIPFLAVVGVVIAYCYFKFGGKSSKGMNLIFEVGHGE
                     EEIIPLRLVPFIISGTWLTHLFGGSAGREGVAVQIGATFSHWVGKRLPIKNACSIFLV
                     TGMAAGFAGLFETPIAAILFAMEVLVAGSLEYQSLFPAFTASFTASSVSKALGLEKFS
                     FALSSKVVFDLSIFWKLIVLGIIFGMVGGAFAWCLKLSKRKIGNRLKNPMIRIAIIGV
                     CLSVLFLLFYKGRYSGLGTNLIQNSFYGGEIYSFDWLLKFILTILTLSAGFQGGEVTP
                     LFSIGASLGVLLAGFFNLPIELVAALGYASVFGSATNTFFAPVFIGAEVFGYSYLPYF
                     FVVCAISYIFNMDKSIYSLQKISTKQ"
     regulatory      complement(3593..3669)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
BASE COUNT         1446 a          734 c          415 g         1393 t
ORIGIN      
        1 actttatata aagaatagaa acaagttcta gattactata gagatgacat atcaaaatat
       61 ttttaaaaag taatttaagt attagaagta attgaagtta gtaaatataa atgcgtttac
      121 ttttaagtat tgcttaaggg aataaaaaat aaaaataaac tctgcaacca tctaaaaata
      181 gttgcagagc ttacaaaacc aaaattatct aaattgtaat aaaaataata acatttatac
      241 ataagcttct tcccaattta ctacccagtt ttctaacgac tttatctttt cctctgttag
      301 ctcctcttcg tgaaatgata ctaataaatt ccttataaaa ttattcttca aaagattttg
      361 cacatttctt gatataaact ctaaatatgc cttttctttt attgcaactg tataatacat
      421 acacttgcct gtttcttgaa catataagaa tcttttcttt gatagttttg ataaagtttt
      481 taaagctact ttttctggta aattatgttc ttctttcata taatcaataa tctcatatga
      541 attggttttt atatctaaat tccaaataaa tttcataata actaactcta attttgataa
      601 tttttttaaa cacatagtta tcactccgct tctttatcta tataattaaa tattacaata
      661 ataatttcac aaaatcaatt ctaataatca taacatacct taaaatctgt tcttaacttc
      721 taccttctta atctcccaaa tgattttttt atattgtatt caatagtata atccttttcc
      781 aaaatataac ttaccagtta aaataatctt tatttttact tactctcgcc ttttacagaa
      841 gttaataatt ttttataagt tacatcaatt ccaaaagctc caagaggtgc agttataagt
      901 atagctagaa ctgctaacgt caaaataagt tgtccagatg ccatccccat tgcaagtggt
      961 acacctccta ttgctgcctg tactgtagcc tttggaatat agccaatcat acagaacaac
     1021 ctttccttta ttgtcaaatt tgtcttaata aggcagaaga atactcctac catacggaat
     1081 aaaagaactc caaatattaa aattacagct ccaattcctg ctttaaatgc ataacttata
     1141 tctactgttg cccctaccaa aacaaacaag agaatttcag cgccaaccca tagttttgaa
     1201 tactttatgg acaatcttct tgcaactaca cgatatttta tctgaaggaa aatcccaata
     1261 ctcataactg ctaataatcc agaaaatggc acaataccct ctaaagcagt ctctaaactt
     1321 atcattaaaa atgagatact taaaagcaac acccctttag cagaatctct catatgaacc
     1381 ttcttaaaaa atactgctaa acagattcca atcactgctc caactattac acctaatata
     1441 attgatactg gaatttgtaa aaaactaata gctgatatac tatttccttg tgccaatcct
     1501 gtgaatactg taaacatcac aattacgaaa atatcgtcta tagaagttcc tgctaaaagc
     1561 atctgtggaa tacttttttc tgtaccataa ccttcttcca ttaatttaag cattttaggt
     1621 acaataattg cgggagaaac tgctcctact actgcaccca tgactgctgc ttccaatata
     1681 gaaacgccta gtaattttgg tgctaaaact atcattccga taatttcaaa agtagctgga
     1741 ataaagcaca taagtacagc aggtcttccc actttcttca aatcgtttat atctaaagat
     1801 aatccagccc ttgttaatat aataatcaat gcaatctttc taaggtcaga agaaatatct
     1861 agtattgaat tatcaatcaa atttaaaaca tatggaccta atataattcc tgtaagtaac
     1921 attcccaata agttaggtaa ctttatcctc ttaaaaatac ctcccgaggc cattcctaat
     1981 aaaaaaatca aagctaaact tgtcaacata ttcctaccct atcctttctc taatattctt
     2041 tctcttctaa tcatacatta tgttgtttta tttattgttt tgttgaaatt ttttgtagtg
     2101 aataaataga tttatccata ttaaatatat atgaaatagc gcatactaca aagaaataag
     2161 gtaaatagct gtatccaaaa acttctgccc ctataaatac aggtgcaaaa aaagtatttg
     2221 tagcactacc aaagacacta gcatagccaa gcgctgcaac taattcaatt ggcaaattaa
     2281 aaaatcctgc taacaataca ccaagactag ctccaatgga aaacaaaggt gtaacctctc
     2341 ctccttgaaa acctgctgac aaagtcagta ttgtaagtat aaactttaac agccaatcaa
     2401 atgaataaat ttctccacca tagaaactat tttgaattaa atttgtccct aaacctgaat
     2461 atcttccttt ataaaataaa aggaataata cactaagaca tactccaata atagcaattc
     2521 ttatcatagg attttttaat ctattaccta tttttctttt tgacaatttc agacaccatg
     2581 caaatgctcc accaaccatt ccaaaaataa ttcctaaaac aataagcttc caaaatattg
     2641 acaagtcaaa tacaacttta gaagaaagtg caaaagaaaa tttctctaac cctaaagctt
     2701 ttgatactga acttgctgta aaagaagcag taaacgcagg aaaaagcgat tgatactcca
     2761 gtgaaccagc taccaaaacc tccatagcaa atagtattgc tgctattggt gtctcaaata
     2821 atcctgcaaa accagctgcc ataccagtta ctagaaaaat actacaagca ttttttatag
     2881 gcaacctttt acctacccaa tgtgaaaaag ttgcaccaat ctgaacagca actccttctc
     2941 gtcctgcact accaccaaat aaatgggtta accatgtacc tgatattata aatggaacca
     3001 atcttaaagg aataatttct tcttctccat gccctacctc aaatattaga ttcattcctt
     3061 tactactttt tcctccaaat ttaaaatagc aataagcaat aacaactcct actactgcta
     3121 aaaatggtat cagatacatt ggatatgttt ctcgaatatc tgtaactttt agaagaaccg
     3181 ttccaaatat ggtgtctata agacctataa ttgcaccaac tggtatacct atgagtccta
     3241 aaaaaaatag tccaccatat gttttataaa cgcttttaat tcttattatc attttaatcc
     3301 atattaaagt tttaaatttt tctatcattt taattccttt ctatttgcat cataagattt
     3361 tttgaaagtt ttattacatc aactgtagtc aatgtaatga ctatcataat tgttttttaa
     3421 attgatagga tataggtaca ctcttaaccc attcttaata aaataattaa catataatct
     3481 actatttttt ctattaatta aatatttctt caaatttaaa aaataaaaat ttagtaagta
     3541 cttcacctcc attttattga tttaaacaac aaaaaaagct ggtggcatct atgatatcat
     3601 agaagtcatc agctcattgg cagtttagga tattatcatc caagggagaa cctcattccc
     3661 ataattatta tataattttt tatttttcat atgtctattt tatgtaaatt gtatttttaa
     3721 tgttataaat aatataacct atcaattaaa atagtaaagc ctctaatatt atgagtgttc
     3781 aatatttcat attattttta tgatatataa tttaaatgat tttctttact ttgcattatg
     3841 atacataact taaatatttt tcctcactcc acattatgat atattattta aatatttttc
     3901 ttcactctgc ttatattatg atacatgatt taaatatttt cttcactctg cttatattat
     3961 gatacatgat ttaaatattt tcttcact
//
LOCUS       DAYAPA010000205         3950 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.210, whole genome shotgun sequence.
ACCESSION   DAYAPA010000205 DAYAPA010000000
VERSION     DAYAPA010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3950
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.210"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(271..666)
                     /locus_tag="ACGI59_003679"
     CDS             complement(271..666)
                     /locus_tag="ACGI59_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HFG9741428.1"
                     /translation="MKALEFLNKAGVYYLATVDENNQAHVRPLGFVMEYNGKLTFCTS
                     NQKDMFKQLIFNPKVELCCIDQNLNTLRILGEAVFVTSKETQAKALEIMPSLGQMYSV
                     GDGKFEIFSIDKAKVSCCSMSGKKIDVEL"
     gene            807..1199
                     /locus_tag="ACGI59_003680"
     CDS             807..1199
                     /locus_tag="ACGI59_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFG9741429.1"
                     /translation="MSINTEKNITKNVEELTCPIRYALDIVGGKWKLPIICMLAVENP
                     IRYSSIKRKLDGITNTMLAQSLKDLESTGIVHRKQYNEIPPKVEYTLTSKGKSIVPIL
                     QQFANWGATNMQEKNTCGLSCKECRKIK"
     gene            complement(1299..1715)
                     /locus_tag="ACGI59_003681"
     CDS             complement(1299..1715)
                     /locus_tag="ACGI59_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HFG9741430.1"
                     /translation="MNWQDAFFQDTKPTFEQINEFINNPLWDKLNKTLVSTYNVEPKL
                     EYSKCSMQRGWNVKYKKRGKSLCTLYPQEGYFKALVIVSESNRVEVDLFINTCCDYIK
                     KIYNEVNFFNGSKWLMIQVDSLLVLNDMLELIKFRA"
     gene            complement(1730..2641)
                     /locus_tag="ACGI59_003682"
     CDS             complement(1730..2641)
                     /locus_tag="ACGI59_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFG9741431.1"
                     /translation="MHAWESIQITLDLIEANLSEEISINELANKANLSPFYYQRLFKR
                     LVNKTVMEYIKLRRLARASEYLAEHKNRILDVALNFGFASHETFTRSFKKAYGLTPEK
                     YRANPVKLNHFIKPELILNYAMVDEGVPLIADDIVIEVNRKTLSNPRTFVGIEIEVPI
                     CQLVGGETTGIAIVEELWMKLGSQRHNIPNQIPGGNEFAALYMGNAKEGNCMYMAGVE
                     VESGTSVEGYSTFELPAKEYLVCGFEAENFNELVNSAVFKADKFMERWMKKHNLTTTD
                     FAIEMYYPTTPEAAYLEHWIVPVPIEQ"
     gene            complement(2823..3845)
                     /gene="splB"
                     /locus_tag="ACGI59_003683"
     CDS             complement(2823..3845)
                     /gene="splB"
                     /locus_tag="ACGI59_003683"
                     /EC_number="4.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006290 - pyrimidine dimer repair [Evidence
                     IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spore photoproduct lyase"
                     /protein_id="HFG9741432.1"
                     /translation="MFNPKRIIFEKGTLDTDVGKKIYNKFRDNKDVEIINISSNRIKE
                     HIPGENLYEQYRSGKQTMVVGIRKSLKFQTCKPSANYQLPLVSGCMGRCEYCYLNTQL
                     GDKPFIRVFVNVDEILEQTKKYIEDRKPQVTIFEGAATSDPVPVEPYTNSLKKTIEFF
                     GREEKGRFRFVTKYSDVDSLLDAEHNNHTEIRFSINTPRIINEHEHYTASANKRIESA
                     VKLARAGYQIGFIIAPVFLYEGWKEEYKNLIISIKEALPKDFDKKIIFEVISHRYTTK
                     AKNRILEIYPDTLLPMENEDRKFKYGQFGYGKYVYDKEQLDDMKEFFLKEINGVFKNS
                     LIKYII"
BASE COUNT         1330 a          666 c          437 g         1517 t
ORIGIN      
        1 taatattttt ttcagattaa ataaaaatca attatagtta aaatattttt taaaagaaat
       61 ctatcccatt ataaagtaaa ttttaattat cttatttcta gtatgtaatt attcaaaata
      121 gtatatcatt attgaaatac acatctaata actaaaaatt tttacatatt cattagttaa
      181 aaatcttttt attgcattta aattaaatct atcaatattt cagtaataac gttttcttaa
      241 ttttcgctat ggatgtcgat tcaattataa ttaaagctct acatcaatct ttttaccact
      301 cattgaacaa caactaactt ttgccttatc aatagagaat atttcaaact taccatctcc
      361 tacagagtac atctgaccta aacttggcat tatttcaagt gccttagctt gtgtttcttt
      421 tgatgtaaca aatactgctt cacctaaaat acgtagagta ttaaggtttt ggtctataca
      481 acaaagttca acctttggat taaaaataag ttgcttaaac atatcctttt gattagatgt
      541 gcaaaatgta agtttaccat tgtattccat aacaaatcct aaaggacgca catgtgcttg
      601 attgttctca tcaactgttg ctaaataata aactcctgcc ttgttcaaaa attctaatgc
      661 tttcataatt cttacacccc ttttaaatac ttgtattaag gattatcctt tgatataatt
      721 ttaatataaa atacagaaaa aacaagtatg cagttttttc tgataaagta taaaaaagat
      781 actactataa ctaaggagta attaaaatga gtataaatac tgaaaaaaat ataactaaaa
      841 acgttgaaga attaacctgt ccaatccgtt atgcattaga cattgtcggt ggtaagtgga
      901 aattacctat tatatgtatg ttagcagttg aaaatccaat tagatatagt agcataaaaa
      961 gaaaattgga tggcattaca aatactatgc tagctcaatc attaaaagat ttagagtcta
     1021 ctggtattgt tcatcgtaaa caatataatg aaattcctcc aaaagttgaa tatacattaa
     1081 caagtaaagg taaaagtatt gtgcctattt tacaacaatt tgctaattgg ggtgctacta
     1141 atatgcaaga aaaaaacaca tgtggactta gttgcaaaga atgtagaaag ataaagtaaa
     1201 tttaaattta ctaaattatt catattatag actatatatt aatttatctg attgaatata
     1261 aaaacaggat gacttatata ttcatcctgt tctatatgtt aagctctaaa tttaattagt
     1321 tcaagcatat cgtttaatac caataaacta tctacttgaa tcattagcca tttacttcca
     1381 ttaaaaaagt ttacctcatt atatattttt ttgatataat cacaacaagt attaataaat
     1441 aaatcaacct ctactctatt gctttcactt acaataacca aagctttaaa ataaccttct
     1501 tgtggatata aagtacatag tgatttacct ctttttttat attttacatt ccaacctctt
     1561 tgcattgaac atttactgta ttcaagctta ggttctacat tataagtaga aaccaaagtt
     1621 ttatttaact tgtcccataa tggattatta ataaattcat taatttgctc aaatgtaggt
     1681 ttagtatctt gaaaaaacgc atcttgccaa ttcatattca tcatcctttc tattgttcaa
     1741 taggaaccgg aacaatccaa tgttccagat atgctgcttc aggtgtagtg gggtaataca
     1801 tctcaattgc aaaatcagta gttgttaaat tatgtttctt catccatctt tccataaatt
     1861 tatctgcttt aaaaacagct gaattaacta actcattaaa gttttctgcc tcaaatccac
     1921 atactaaata ttctttagct ggcaactcaa aagtactata tccttctact gatgtacctg
     1981 actctacctc tacacctgcc atatacatac aatttccttc ttttgcattt cccatataaa
     2041 gagcagcaaa ttcatttcca cctggaattt gatttggtat attatgtctt tgacttccaa
     2101 gtttcatcca taattcctct actattgcaa ttccagttgt ttcaccgccc accaactggc
     2161 aaataggtac ttctatttct attccaacaa atgtccttgg attactcaat gtcttacgat
     2221 tgacttcaat cactatatca tctgcaatca gtggaacacc ttcatcaacc atagcataat
     2281 ttaaaattaa ttctggctta ataaaatgat tgagttttac tggatttgct ctatattttt
     2341 ctggagttaa tccatatgct tttttaaagg aacgtgtaaa tgtctcatga ctagcaaatc
     2401 caaaatttaa agctacatca agtatacgat tcttatgttc tgctaagtat tcagaagctc
     2461 ttgcaagacg acgaagcttt atatactcca ttacagtctt atttaccaat cttttaaata
     2521 gtctttgata gtaaaatggt gataaatttg ctttatttgc taattcatta attgatattt
     2581 cttctgataa atttgcttct attaaatcca atgtaatttg aatagactcc catgcatgca
     2641 tactataact cctcctgata tttttgttac tcttatcctt tatagtatca aaactagaaa
     2701 aaatgttctt gatagatagt gcccttttta attttcttat atgataatgc taaatctcat
     2761 tgctttatta taaacataaa atatataaaa ataaaatata gcaaatcttt aggtaagatt
     2821 tgctatatta tatattttat taaactattt ttaaatactc catttatttc cttaagaaag
     2881 aactctttca tatcatctaa ttgttcttta tcatacacgt acttaccata tccaaattga
     2941 ccgtatttaa actttctatc ttcattttcc attggcaata gtgtatctgg ataaatctct
     3001 agtattctat tttttgcttt agttgtatat ctatgagata taacttcaaa tataattttt
     3061 ttatcaaaat cctttggtaa agcttctttt atacttataa tcagattttt atactcttct
     3121 ttccaacctt catataaaaa cacaggtgct attatgaaac caatctgata accagcccta
     3181 gcaagtttta cagctgactc tattctttta tttgcagagg ctgtataatg ctcatgttca
     3241 tttataattc taggagtatt gatactaaat cgtatttctg tatgattatt atgttcagca
     3301 tccaataagc tatccacatc actgtattta gtaacaaatc taaatcttcc tttttcctct
     3361 cttccaaaaa actctattgt tttcttcaaa gaattagtat atggctctac tggaactggg
     3421 tctgatgtag ctgccccttc aaatatggtt acctgtggct ttctatcttc aatatatttt
     3481 tttgtctgtt caagtatttc atctacattt acaaacaccc ttataaatgg tttatcacct
     3541 aattgtgtgt tcaaataaca atactcacat cttcccatac atccactgac aagtggtagt
     3601 tgataattag cagaaggttt acatgtttga aacttcagac tttttctaat cccaactacc
     3661 atagtctgct tcccacttct atattgttca tatagatttt cacctggaat atgttcttta
     3721 atcctatttg atgatatatt aattatctct acatctttat tatctctaaa tttattgtat
     3781 atttttttac ccacatcagt gtctaaagtt cctttttcga atattattct tttgggatta
     3841 aacatttatt tcacctctac ttaaagtttt taaatcgaag aattttataa aatattactt
     3901 aattaatctt ccctttttta aactccttta tttattagaa agtaaaataa
//
LOCUS       DAYAPA010000206         3895 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.211, whole genome shotgun sequence.
ACCESSION   DAYAPA010000206 DAYAPA010000000
VERSION     DAYAPA010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3895
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.211"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            30..902
                     /locus_tag="ACGI59_003684"
     CDS             30..902
                     /locus_tag="ACGI59_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9741433.1"
                     /translation="MEMKIAVSNLSKRFGKKQALDNINLEIDSGMFGLLGRNGAGKTT
                     FMRILATLLSKTEGSIHMCGVPIENTKEIRNMVGYLPQDFSMYPNMTVYKAMDYLAVL
                     SDIPLRKRRKIIAKLLSKVNLTNYQNVKVKSLSGGMNRRLGIAQALIHNPRVLIVDEP
                     TAGLDPEERIRFRNLLCEIANDRIVILSTHIVGDIEATCENVAILNSGKVIYNGSTEA
                     LAKLAEGKVYSIEVDKKDIENIKSRFIVIGMLTHGGKAILRIISDDKPFETAVNCNPT
                     IEDGYMLIMGGDNI"
     gene            899..2188
                     /locus_tag="ACGI59_003685"
     CDS             899..2188
                     /locus_tag="ACGI59_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9741434.1"
                     /translation="MNLFIKECKNILKSMVYYIFIVVLVFFYISQLGSSVSNDVSKNI
                     PNDGNPLVAPQKVENIEEYYSVHNQYPYGQKESMKTEKVIPNATINLIREYQKNTYTT
                     YPTGVVKYIKLDQNKTKTIEKYIEEITGKSISEINSLWKTDESIKIDYSKVSLERFEE
                     IMQRVDDILGGGSAYAFDKLQRFGVVPVTYDEAMKNYNYTIQKDKVSNGYARIFCDYI
                     GIVLSIFPVFVAVFMSLKDKRSRMREIIYSKQVSSCKIVLSRYFALVFMMILPVLILG
                     MKDLLPLIFFAKDKGISIDNLAFIKYTLWWILPTLMIVTAVGMSITILTDTPVAIIVQ
                     LIWWLYSINSTGLKGDYSTMGLMIRHNSVLNGDIIIQNFNQIMINRIVISLGALVLVL
                     LAIFLYEQKRRGNLDVWAKFSKLFSSNKRKLQIKHFN"
     gene            2118..2762
                     /locus_tag="ACGI59_003686"
     CDS             2118..2762
                     /locus_tag="ACGI59_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9741435.1"
                     /translation="MFGQSFQNCLVLIKENFKLSTLTNLICSIILLCMVPLIQGVANL
                     DRISTAICLENYVAIIGIVMLVPVFSLEQSKEIDEIVSSKALSQVIVQLIRILMSIIG
                     IVVLIIGFVNVLIYLGCNFPVVEYIIGSFASAMFIGSLGMFFSNLFNNTIIGYMISIG
                     YLILNMMTKGKYVGKFFIMSMSQGSFDEKYWLILGSVVLIIASLLIKPVKRKIR"
     gene            complement(3029..3493)
                     /locus_tag="ACGI59_003687"
     CDS             complement(3029..3493)
                     /locus_tag="ACGI59_003687"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFG9741436.1"
                     /translation="MNLNKVKFTEEHAIEVTNWKYEGKYSIYNLPPWEEIKKKNFSLA
                     KEDKRKNFISFINDNKELIGFINLLDEGSSVFFGIGMKPNYCGMGIGKEIIGLGLQEC
                     RNKYSTKPIVLNVRTWNMRAVKCYESQGFKIVETKVQKTHLGDGEFFVMRYQ"
BASE COUNT         1453 a          460 c          601 g         1381 t
ORIGIN      
        1 taaaattgtt tctgggggtg tatttatata tggaaatgaa aatagcagta tccaatctta
       61 gtaaaagatt tgggaaaaaa caagctcttg ataatataaa tcttgaaatc gacagtggta
      121 tgtttggtct tttaggaaga aatggagcag gaaaaaccac atttatgaga attttagcca
      181 ctttattatc aaaaacagaa ggttcaatac atatgtgtgg agttcctatt gagaatacta
      241 aggaaattag aaatatggtt ggttatttgc cacaagattt ttcaatgtat cctaacatga
      301 cagtatataa agcaatggat tatttagcag ttttatcgga tataccacta agaaaaagaa
      361 gaaaaattat agcaaagtta ttaagcaaag taaaccttac taattatcaa aatgtgaaag
      421 taaaatcttt atctggaggt atgaatagaa ggcttgggat agcacaagca ttaattcata
      481 atcccagagt tttaatagta gatgagccga cagcaggtct tgacccagaa gaacgtataa
      541 gatttagaaa tttactatgc gaaattgcaa atgatagaat cgttatttta tctacacata
      601 ttgttggaga tatagaagct acatgtgaaa acgtagctat attgaatagt ggaaaagtca
      661 tatacaatgg ctcaacagaa gcattagcaa aactagctga agggaaagta tatagtattg
      721 aagtagataa aaaagacatt gaaaatataa aaagtagatt tatagttata ggaatgttga
      781 ctcatggtgg aaaggctata cttaggatta tatcagatga taaacctttt gagacagcag
      841 taaattgtaa tccaactatt gaagatggct atatgcttat aatgggagga gataatatat
      901 gaatctattt ataaaagaat gtaaaaatat attaaagagt atggtatatt atatttttat
      961 tgtagtttta gtttttttct atatttctca acttggaagt tcagtttcaa atgatgtaag
     1021 taaaaatatt cctaatgatg gaaatccatt agttgctcca caaaaagtag agaatattga
     1081 ggaatattat tctgtacaca atcaatatcc atatggtcaa aaagaatcta tgaaaacaga
     1141 aaaggtcatt ccaaatgcaa ctataaacct aattagagaa tatcaaaaaa acacatatac
     1201 aacgtatcca acaggggtag ttaagtatat aaaattagac caaaataaaa cgaagaccat
     1261 agaaaaatat atagaagaaa taactggtaa aagtattagc gaaataaatt cactttggaa
     1321 aactgatgaa agtataaaaa tagattattc taaagtatct cttgaaagat ttgaagaaat
     1381 aatgcaaaga gtagacgaca ttttaggagg agggtctgca tatgcttttg acaagttaca
     1441 aagatttgga gtagttccag ttacctatga tgaagctatg aagaattaca actatacaat
     1501 acaaaaagat aaagttagta atgggtatgc aagaattttt tgcgattata taggtatagt
     1561 tttaagtata tttccagtat ttgtggcagt ttttatgtca cttaaagata agcgttcaag
     1621 aatgagagag attatttatt caaaacaagt atcttcatgc aaaatagttt tatcaagata
     1681 ttttgcactt gtctttatga tgatattgcc tgtattaatt ttaggtatga aagatttact
     1741 tcctttgatt ttctttgcta aagataaagg tatatctatt gataacttag catttataaa
     1801 atatacatta tggtggatac taccaactct tatgatagtt acagctgtag gaatgagtat
     1861 tactatatta acagatacac cagtagcaat tattgtgcaa ttaatttggt ggctttacag
     1921 tataaattct acaggactca aaggtgatta ttcaacaatg gggctaatga taagacataa
     1981 tagtgtatta aatggggata ttattataca aaacttcaat caaattatga ttaatagaat
     2041 tgtgataagc ttgggagcat tggtgcttgt tttgttagct atattcttat atgagcaaaa
     2101 aagaaggggg aatcttgatg tttgggcaaa gttttcaaaa ctgtttagtt ctaataaaag
     2161 aaaacttcaa attaagcact ttaactaatt taatatgttc aattatattg ctatgtatgg
     2221 ttcctctaat acaaggagta gctaatttag acagaatttc aacagctata tgtttagaaa
     2281 attatgttgc aataataggg atagtaatgt tggtacctgt attttcgcta gagcaatcaa
     2341 aagaaattga tgagatagta tcttcaaaag cattatctca agttattgta caattaatta
     2401 ggatactgat gtcaattata ggtatagtgg tattaatcat aggttttgta aatgttttaa
     2461 tatatcttgg atgtaatttt ccagtagttg agtatattat tggaagtttc gcatcagcta
     2521 tgtttatagg aagtttagga atgtttttct caaatttatt taataataca atcataggtt
     2581 atatgatatc tataggatat ttaattttaa atatgatgac taaaggcaag tatgttggaa
     2641 aattttttat aatgtctatg agtcaaggta gttttgatga gaaatattgg ctgatacttg
     2701 gaagtgttgt gttgataatt gcatctctct taataaaacc agtaaaaagg aagattagat
     2761 aataaaatga gagcttagat aaaataaagg taatacaaat aaaaagtaca tttatgaaat
     2821 ataaatctat ttttatatac acaaacctac ttacaatctc tttatatatg tagatagatt
     2881 tatatatccg tagtataaaa tatatatgta tacaaaattt tatcaaaagc tctcaaattt
     2941 atatttcaaa gacacttatt gctatgacct acacatatgt attttttcat tttaattgtt
     3001 gtattaacaa taagactatt gttataaact attgatacct cataacaaaa aattctccat
     3061 ctcctaaatg cgttttttgt accttagtct caactatttt aaatccttga gactcataac
     3121 attttacagc tctcatgttc caggttctta catttaatac aattggttta gtactatatt
     3181 tatttctaca ttcttgtaat cctaggccaa ttatttcttt accaattccc attccacaat
     3241 aattcggttt cataccaata ccaaagaata cagagcttcc ttcatctaat aagtttataa
     3301 aacctatcaa ttctttattg tcatttataa aacttataaa attttttctt ttatcttctt
     3361 ttgctagtga aaaattcttc tttttaattt cctcccatgg aggtagattg tatattgaat
     3421 atttaccttc atatttccaa ttagtaactt caatagcatg ttcttcagta aatttaactt
     3481 tatttaaatt catatgtcct cctattaaaa tatttaaggt aattaatggt attttaaagt
     3541 tttagaaaat aagtaaaact atttatattt atccataaaa tctttaaaaa ttttactaac
     3601 aattcttgta ccctgtactt tttattggat tgacagaaga ctataaacta attttgaatt
     3661 accttctaat tcaaaaacat cacgatatat atataatttc ttagcttgat taaattctgt
     3721 taaatccgtt tcttatagtt gtataatcaa catcaatatc tttatgtgta ttagttaatt
     3781 tattggtttt aggaacttat tattaatata ttgtatttgc tccaataatc caagtagtag
     3841 gtaaattata ggcaaattat caaatgtata tctatccatg cttaaacttt agaga
//
LOCUS       DAYAPA010000207         3884 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.212, whole genome shotgun sequence.
ACCESSION   DAYAPA010000207 DAYAPA010000000
VERSION     DAYAPA010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3884
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.212"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(105..323)
                     /locus_tag="ACGI59_003688"
     CDS             complement(105..323)
                     /locus_tag="ACGI59_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFG9741437.1"
                     /translation="MSVLLKTKIKSYREKNLMTQGELAKSVGVRRETIVHLENGRYNP
                     SLKLAMDIAKVFDTTVEELFEFIEENNI"
     gene            complement(324..731)
                     /locus_tag="ACGI59_003689"
     CDS             complement(324..731)
                     /locus_tag="ACGI59_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9741438.1"
                     /translation="MSKQQTGIKSTLISEILGISYCIGATLVNKYFFKGSSIEYLPII
                     IWVLAMGAQWVYNKKYDDLIDEASKCILSRVNDIAIKVLFFSVAIVSIFLVTLDINVS
                     NLNIGMSLLVILFIQSLLKLILFIYFDRKGIYN"
     gene            complement(817..1305)
                     /locus_tag="ACGI59_003690"
     CDS             complement(817..1305)
                     /locus_tag="ACGI59_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3841 domain-containing protein"
                     /protein_id="HFG9741439.1"
                     /translation="MKFYTIQTLEFWNNNKNNKYLSNDYSFILEDFDIPYKWMYSQMT
                     NRIYEFDDSMIWVWTKRPDLRCSGYLEKGKKGVLLEIEISEDNVLLSEFDLWHLPLMD
                     ITVELYEDEQIEKKVSWERIFDLELCSKLNEVNIEDLTLQGVTSKVPLSNVKLIKEFV
                     AK"
     gene            complement(1510..2607)
                     /locus_tag="ACGI59_003691"
     CDS             complement(1510..2607)
                     /locus_tag="ACGI59_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFG9741440.1"
                     /translation="MKEKLEEKSKIIKTSKEYKRTIIEEKDREWTKKLNDILSRGDFS
                     NEKLDKIKDLIPKEILTSENVGEVVTEDGYAKPEYDEVFKSLFTLNNDYDLLANFIND
                     ILKDAPYANRNIKQFTQIKRIIKVETDPTINYIGEKRPRLDILAEDEERNHINIEMQR
                     ALEEDYLERAEYYLSRVHGRKLEEGKEYKEIGKTVGIHILNHVKYNHIEDYVNCLRLT
                     MDGHPDIYSSKTALYFIELPKIRKSSCIANRVLIWGKLIDNPSHIDIRILSKTDYVIK
                     RALDRLKELGSNEDYLLNLKRGAYIMNKSRNFKEEIFQEGIEKGIAKGKREEKFNIII
                     KLKNKGYNLSEICDIIDDLNKSEVEKVYNQN"
     gene            complement(2980..3150)
                     /locus_tag="ACGI59_003692"
     CDS             complement(2980..3150)
                     /locus_tag="ACGI59_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9741441.1"
                     /translation="MDSNNNTIKSTVKKGISFGSCLAMIISYTAWKSIPWAIFHGLMS
                     WIYVLYYWVKYA"
     gene            complement(3322..3765)
                     /locus_tag="ACGI59_003693"
     CDS             complement(3322..3765)
                     /locus_tag="ACGI59_003693"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFG9741442.1"
                     /translation="MIEIKTIEINKKSRIDLLMLADPCEEMIDKYLNKGTMYALYDNK
                     ELTCIAVVNEISKEICELKNIATYEHFQNMGYASKMIYHLLDVYSKKYSSMVVGTSES
                     GVPFYEKFGFVYSHKIKNFFVDNYPEPIFEGELQCVDMLYLSYNF"
BASE COUNT         1328 a          637 c          352 g         1567 t
ORIGIN      
        1 atgagcttgg tttttcatct ataagtcatt tattctctaa tctataagct tggtttttta
       61 cctataatta attcattctc taatgcataa gtaatacatg aaatttaaat attattttct
      121 tcaataaatt caaacaactc ttctacagtt gtatcaaata ccttagcaat atccattgcc
      181 agttttaaag aaggattgta gcgaccattt tctaaatgta caatagtttc acgtcttact
      241 cctactgact ttgcaagttc accttgtgtc ataaggttct tttctctata ggatttaatt
      301 tttgtcttta gtaatactga cacctaattg tatatcccct ttctgtcaaa atatataaat
      361 agaattaact tcaacaagga ttgaataaac aaaatgacta ataaagacat tcctatattt
      421 aaatttgaca catttatgtc caaagttaca agaaatatag atactatagc tacagaaaaa
      481 aacaatactt ttattgcaat atcattaact cttgatagta tacattttga tgcctcatca
      541 attaaatcat catacttttt attatagacc cactgagctc ccatagctaa tacccaaata
      601 attataggaa gatattcaat actacttcct ttaaaaaaat acttatttac taatgtagct
      661 ccaatacaat aagaaattcc caatatttct gaaataagtg ttgatttaat accagtttgt
      721 tgtttagaca tataaaatcc cccttaaaaa gttatgtatt tataacacct cttgttataa
      781 atatataact tttttgaata aatttcaata aaaatattat tttgcaacaa attcttttat
      841 taattttaca ttagatagag gcactttaga tgtcactcct tgcaatgtca aatcttctat
      901 attaacttca tttaattttg aacacagttc taagtcaaat attctttccc agcttacctt
      961 cttttcaatt tgttcatctt cataaagctc tacagtaata tccattaaag gtaaatgcca
     1021 taaatcaaat tctgataata acacattgtc ttcacttatt tctatttcaa gtaaaacacc
     1081 tttttttcct ttttctaaat aaccactaca tcttaaatca ggtctcttcg tccaaaccca
     1141 tatcatacta tcatcaaatt catatattct atttgtcatt tgactataca tccatttgta
     1201 tggaatatca aagtcttcta atataaaaga ataatcattt gatagatatt tattgttttt
     1261 attgttattc caaaattcta aagtctgtat agtataaaat ttcatatata aatcaacccc
     1321 tttgtttttc tataagaagg agatggttca aacttttatt ttgaattatc tcctttattt
     1381 atacacctct ttgtacctat tatttatata tctctttata tctaccatat ttaaataaca
     1441 aagttttata ttaatttaaa tttaaaagag gaagctttgt atttatctag tgccatctca
     1501 atcatagtat tagttttgat tatagacttt ttcaacctct gatttattca aatcatctat
     1561 aatatcgcat atttcactca aattgtatcc tttatttttt aattttatga tgatattaaa
     1621 tttctcttct cttttccctt ttgcaatacc tttttcaata ccttcttgaa aaatttcttc
     1681 tttaaaattt ctacttttat tcataatata cgcacctctc tttaaattta ataaatagtc
     1741 ctcattacta cctaattctt ttaatctatc aagtgctctt ttaataacat aatccgtttt
     1801 agatagtatt ctaatatcta tatgagaagg attatcaata agtttacccc atattagaac
     1861 tctgtttgct atacaactgg atttacgaat ttttggaagt tctataaaat ataatgcagt
     1921 tttactagaa taaatatctg gatgcccgtc cattgttaat ctcaggcaat ttacataatc
     1981 ttctatgtga ttatatttta catgatttag tatatgtatt ccaacagttt ttccaatttc
     2041 tttatattct ttaccttctt ctaactttct accatgtaca cgactaagat aatattcagc
     2101 tctttccaaa taatcctctt caagagctct ttgcatctca atatttatat ggttacgttc
     2161 ttcatcttca gccaaaatat caagtctagg tcttttctca ccaatgtaat taattgttgg
     2221 gtcggtttca accttaatta ttctttttat ttgagtgaac tgtttgatat tcctattagc
     2281 atatggagca tctttgagga tatcatttat aaaatttgca agtaaatcgt aatcattatt
     2341 taaagtaaac aatgatttaa atacctcatc atactcaggc ttagcatacc catcttcagt
     2401 tacaacttct ccaacatttt cactagtcaa tatttctttt ggaattaaat ccttgatttt
     2461 atctaatttt tcattagaaa aatcacctct actaagtatg tcatttaatt ttttggtcca
     2521 ttctctgtcc ttttcttcaa ttatagttcg tttatactct tttgatgttt taataatctt
     2581 agatttttct tctaattttt ctttcatatc aacacctctt ttttattatt acccatttct
     2641 tcataccaaa gtctgaactt ctaagtaaaa tttgcattat tgaaataaga accttataaa
     2701 cttatatatc attactaaag ttaagacttt aaagagaagc acttagatta caatagattt
     2761 tagatagctt tctttttaaa actattgttg ataaatgaat gaatataagg gtatgtatta
     2821 aaatagagat actcatcttt ttcattgttt aaataatgaa aatatatttt atttaataca
     2881 aaaaagagtg ccttataaac taaaagttca cttcgagaca ctcccttaca ttttgtttct
     2941 taaactttac aatattacat acttatttat taatttaatc tatgcatact taacccaata
     3001 ataaagtaca tatatccaac tcattaagcc atgaaaaata gcccatggaa tagatttcca
     3061 tgcagtataa gaaataatca ttgctaaaca agaaccaaaa gaaataccct ttttaacagt
     3121 tgattttata gtattattat ttgaatccat aattaatcct ccaaaattca aaataaacaa
     3181 gttattatat atcttatttg aatataatac aatataacag cttctaaata aattatactc
     3241 ttattataca taatttataa attataattc cgataattat atgaattaat taattatatt
     3301 aacctaaaaa tattatccta tctaaaagtt atatgataga tacaacatgt caacacattg
     3361 taattctcct tcaaagattg gttctggata attatctaca aaaaagtttt taattttatg
     3421 tgaataaaca aatccaaact tctcataaaa aggaactccg ctttcagatg taccaacaac
     3481 cattgatgaa tattttttac tataaacatc taaaagatga taaatcatct ttgaggcata
     3541 acccatattt tgaaaatgct catatgtagc aatgtttttt aattcacata tttctttgga
     3601 aatttcattt acaactgcta tacaagtaag ttctttatta tcatacaatg cgtacatagt
     3661 tcctttattg agatatttat caatcatttc ttcacatggg tcggctaaca ttaaaaggtc
     3721 aattcgactt tttttattta tttctatagt ttttatttct atcataagtt ctccaccctc
     3781 actttattca aaccttaatt taacaaactt cttacagtac ttatttaatt cattttgata
     3841 tatattttta ctttttaaaa tatagtgata attccctaga atat
//
LOCUS       DAYAPA010000208         3837 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.213, whole genome shotgun sequence.
ACCESSION   DAYAPA010000208 DAYAPA010000000
VERSION     DAYAPA010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3837
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.213"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            99..1646
                     /locus_tag="ACGI59_003694"
     CDS             99..1646
                     /locus_tag="ACGI59_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HFG9741443.1"
                     /translation="MNKNSMTRIYSLNKIIFIIIGILGIVLLTRHIEVDIKVLILILL
                     TLFSINVFISYLKIKLYEDKINLGLINNSDKFINITKDENFLKHVQNYIISNEKENCV
                     MACFDLCRLKLINDIYGYELGDEVLNNILSNLKNYFGKEAIYGKLKSDVFVLIIKLEN
                     REQKIPYLVNLIKNKIVILSQIDSFKMDINIEASIGIYKFNNNEIDIKKAIDNADMAR
                     LKSKGLKHIEYVEFDNAMEEEIQSIMKIERDLFLAIKNKEFVIHYQPKVDSSTGNIIG
                     SEALIRWLHPSLGMVGPNKFIPIAEKNGLINNIGRWLIQEVFITINKWISEGINVLPV
                     SINLSRVELYKNDLVDFFKLMFNTYNIPKELIELEITETTALRDVKFISERLYEIKSL
                     GMNISLDDFGVGNSNFINLKGIPLDIIKIDRSLILDIVTNTKTKFMVKAIVNLSHDLN
                     VTVICEGVEDMHQVKVLSELGCNFIQGYVFFKPLDEMNYKKLLTDGSYINLKDTLLDK
                     CMTVEEE"
     gene            1649..2047
                     /locus_tag="ACGI59_003695"
     CDS             1649..2047
                     /locus_tag="ACGI59_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HFG9741444.1"
                     /translation="MISEDVDYSIRIIRAIFNNGEDKPIDAKMILKSEDIPILTLYRL
                     LRKLCANNILTFNKEDGKTYMLNVSLDTISLRTIVEVIDGKIYINKCTRYVSNCSNCL
                     KGCKVNRELLEIEKEIYKLLSRKSIRQILE"
     gene            2087..3820
                     /locus_tag="ACGI59_003696"
     CDS             2087..3820
                     /locus_tag="ACGI59_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFG9741445.1"
                     /translation="MDDISQDNFLLSKEYENSLDVDTKKASGIYYTPKIIVDYIVKKT
                     LKNHDIIKNPYPRILDISCGCGNFLLEVYDILYDLFEENIYELKKKYDENYWTVDNIH
                     RHILNYCIYGADIDEKAISILKDSLTNKKVVNDLDESDIKINLFCCDSLKKKWRYKFD
                     YIVGNPPYIGHKKLEKKYKKFLLEKYSEVYKDKADLYFCFYKKIIDILKQGGIGSVIT
                     PRYFLESLSGKDLREYIKSNVNVQEIVDFLGANIFKNIGVSSCILTFDKKKTKETYID
                     VFKIKNEDICINKFETLEELLKSSKFEHFNINQRLLSDEWILVNKDDETFYNKIQEKC
                     KYSLEDIAISFQGIITGCDKAFILSKDDVKLNLVDDKFLKCWIKSKNINKYIVDKSEY
                     RLIYSNDIDNENTNKRILDEIIGLYKTKLENRRECKSGIRKWYELQWGREKLFFERKK
                     IMYPYKSNENRFAIDYDNNFSSADVYSFFIKEEYLDKFSYEYLVGILNSSVYDKYFKI
                     TAKKMSKNIYDYYPNKVMKIRIFRDNNYEEIENLSKQIISILLNKSIDKGKVEKLQIK
                     MDNLIMDSLGI"
BASE COUNT         1613 a          311 c          636 g         1277 t
ORIGIN      
        1 tgtgatttta acttgcattg atttgctatc aattgtgctt tataattagt atatataaag
       61 tttaactgta agataattta aaaaataggg gaatttatat gaataaaaat agcatgacaa
      121 gaatttacag tttaaataaa ataattttta ttataattgg aattttagga atagttttac
      181 tcacaagaca tattgaggta gatattaaag ttttaatttt aattttatta accttgttta
      241 gtataaatgt atttatttca tatcttaaaa ttaagttgta tgaggataaa attaatttag
      301 gattgataaa taattcagat aagtttataa atattactaa agatgagaac ttcctaaaac
      361 atgttcaaaa ttatattatc agtaatgaaa aagaaaactg tgtaatggct tgttttgatt
      421 tatgtagact caaacttata aatgatatat atggttatga gctaggggat gaagtgttaa
      481 ataatatttt aagtaactta aaaaattatt ttggaaaaga agctatttat ggaaagttaa
      541 aaagtgatgt ttttgtatta ataataaagc ttgaaaatag ggaacagaag attccttatt
      601 tagtaaatct tattaagaat aaaattgtga tactaagtca gattgacagt tttaaaatgg
      661 atataaatat tgaggcttct attggaattt ataaatttaa caataatgaa attgatataa
      721 aaaaagctat agataatgct gatatggcaa gacttaaatc aaaaggacta aagcacatag
      781 agtatgtaga atttgataat gctatggaag aagaaataca atcaattatg aaaatagaga
      841 gagatttatt cttggctatt aaaaataaag agtttgtaat tcactatcaa cctaaagttg
      901 attcaagtac aggtaacatt ataggttcag aggcattgat aagatggttg cacccatcat
      961 taggcatggt tggaccaaat aagtttatac ctatagctga aaaaaatggt ctgataaaca
     1021 atattggtag atggctaatt caagaagttt ttattaccat taacaagtgg atttcagaag
     1081 gtataaatgt cttaccagtc tcaataaatc tttctagagt agaactatat aaaaatgatt
     1141 tagttgattt ttttaaactg atgtttaata catataatat tccaaaagaa cttatagaat
     1201 tagaaattac agaaacgaca gcactaagag atgtaaaatt tataagtgaa aggctttatg
     1261 agattaaatc tttaggtatg aatatatcat tagatgattt tggagtaggt aattcaaatt
     1321 ttatcaacct taaggggata ccactagata ttataaaaat tgataggtca cttatattgg
     1381 atatagttac aaatacaaaa acaaagttta tggtgaaagc aattgttaac ctttcacatg
     1441 atttaaacgt aactgttata tgtgaagggg tagaggatat gcatcaggtc aaagtcttaa
     1501 gtgaactggg atgtaatttc atacaaggtt atgtattttt taaacctttg gatgagatga
     1561 attataaaaa acttcttact gacggaagtt atataaatct gaaagatact cttttagata
     1621 aatgcatgac agttgaggag gagtaaggat gatttcagaa gatgtagatt attcaataag
     1681 aatcataaga gctatattta ataatggaga agataaacca atagatgcta aaatgatatt
     1741 aaaaagtgaa gatataccaa tactaacact atacagactg cttagaaagc tttgtgcaaa
     1801 caatattctt acttttaaca aagaagatgg caagacatac atgttaaatg tgtcattgga
     1861 tacaataagt ctaagaacta ttgtagaagt aattgatgga aaaatatata taaataaatg
     1921 tacgagatat gtatctaatt gcagtaattg cttaaaaggt tgtaaagtaa atcgtgaatt
     1981 gctagaaata gaaaaagaaa tttataaact tttatcgaga aaatctataa gacaaatact
     2041 agaataaaaa attttcagct atgataataa ttggaggttt tcggttatgg atgatatatc
     2101 tcaagataat tttctactgt ctaaagagta tgagaatagc ttagatgtag atactaaaaa
     2161 agcaagtgga atatattata ctcctaaaat aattgtagat tatattgtaa aaaaaacttt
     2221 aaaaaatcac gatataatta aaaatccata tccacgaata cttgatattt cctgtgggtg
     2281 tggaaatttt cttttagaag tatatgatat tttatatgac ttatttgagg aaaatatata
     2341 tgaattgaaa aagaagtatg atgaaaatta ttggactgtt gataacatac accgtcatat
     2401 attaaactat tgtatatatg gtgctgatat tgatgaaaaa gctataagta tattaaaaga
     2461 tagcttaaca aataaaaagg tagtaaatga tttagatgaa tctgatataa agattaatct
     2521 attttgttgt gatagtctta aaaagaaatg gcgatataag tttgattaca tagtgggtaa
     2581 tccaccctat attggtcata aaaaacttga aaagaaatat aaaaaatttc tcctagaaaa
     2641 atatagtgaa gtttataagg ataaagctga cttatatttt tgtttttata aaaagattat
     2701 agatatttta aagcaaggag ggattggcag tgttataact ccaagatatt ttttagaaag
     2761 tctatcaggt aaagatttaa gagaatatat taaatcaaat gtcaatgttc aagaaatagt
     2821 ggatttttta ggtgcaaata tatttaaaaa tataggggta tctagttgta ttctaacttt
     2881 tgataagaaa aaaacaaaag agacatatat agatgtattt aaaatcaaaa atgaggatat
     2941 atgtataaat aaatttgaaa cattggaaga acttctaaag agtagcaaat ttgaacattt
     3001 taatataaat cagagattgc tatctgacga atggatactt gtaaataaag atgatgaaac
     3061 tttttataat aagatacaag aaaaatgtaa atatagcttg gaagatatag caataagttt
     3121 tcaaggaata attacagggt gtgataaagc gtttatttta agtaaagatg atgtaaagtt
     3181 gaaccttgta gatgataaat ttttaaaatg ttggataaaa agtaagaata taaataaata
     3241 tatagtagat aaaagtgaat ataggctaat atactcaaat gatatagata atgagaatac
     3301 aaataaaagg atactagatg aaattatagg gctatataaa acaaaattag aaaacagaag
     3361 agaatgtaaa agtggaataa ggaaatggta tgaacttcaa tggggtagag aaaagctttt
     3421 ctttgaaaga aaaaagataa tgtatcctta taaatcaaat gaaaatagat ttgctataga
     3481 ttatgataat aatttttcaa gtgcagatgt atattctttt tttataaaag aagagtattt
     3541 agataaattt tcatatgaat atctagttgg aattttaaat tccagtgttt atgataaata
     3601 ctttaaaata actgctaaaa aaatgagtaa aaatatatat gattactatc ctaataaggt
     3661 tatgaagata aggattttta gagataataa ttatgaagag atagagaatt tatcaaagca
     3721 aataatatca attttattaa ataagtctat agataaagga aaagtagaaa aattgcagat
     3781 aaaaatggat aacttgataa tggattcttt aggtatataa gcagttgtaa gtattaa
//
LOCUS       DAYAPA010000209         3815 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.214, whole genome shotgun sequence.
ACCESSION   DAYAPA010000209 DAYAPA010000000
VERSION     DAYAPA010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3815
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.214"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            142..657
                     /locus_tag="ACGI59_003697"
     CDS             142..657
                     /locus_tag="ACGI59_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFG9741446.1"
                     /translation="MNDARIISQIKAGKKELFDTLIKKYYDSIYCYCYRHVDNKMTAQ
                     DLTQDVFVTVINNIENYKHYGKFENYLYVIAKNKCKDFWKKKQPIYLESLDKEISNQE
                     LSDIENKMFIKEALTKLPELEFEVIILRYYHDLRFKDIAKIMESSVSVTKYRMSRALK
                     KLELYLEGGKL"
     gene            654..1415
                     /locus_tag="ACGI59_003698"
     CDS             654..1415
                     /locus_tag="ACGI59_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9741447.1"
                     /translation="MNNKEIKRSLQKYKVPKYDSSALEKTIKTIHELTLSNTYKRMNN
                     KDFFISQIGFIRKRTWIMQFLILCTICIFLFSTSADSNSFYNSLSLISIASPLFVLTN
                     IEELTKVYNSSILEIEFSTKNTLHKIIATRMLIFGISDLLFMIIILVSAKQIIDVNIL
                     YIIIYILVPFNLMCIGCMEIINRNKGKHTNYYCMIYGISLVLLIVTLSNFRDNLYQFE
                     AINQWLIVFLVTAVTLIYEIRKTWRHLCSFENIII"
     gene            1453..2319
                     /locus_tag="ACGI59_003699"
     CDS             1453..2319
                     /locus_tag="ACGI59_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9741448.1"
                     /translation="MELSVERLTKQYNNKIIVDKIDFKLNEGIYGFLGENGAGKTTLM
                     RMICGILSPTSGEVKVDGKNNLDMGEDFRDMIGYLPQNFGYYPDFTAKEFMLYIASLK
                     GLMPSYAKRKTQELLEMVGLSEVINKKIRTFSGGMKQRLGFAQTLLNDPKILILDEPT
                     AGLDPKERVRFRNLISEFSKEKIVILSTHIVSDVEYIADKILIMKKGRFVLTGSIEEV
                     TSKIKDKVWKCVVNENEIDYMSDRYCLANIHHSGGVANLRIVSDIKPHDNACNLKPTL
                     EDLYLYCFNEVL"
     gene            2340..3581
                     /locus_tag="ACGI59_003700"
     CDS             2340..3581
                     /locus_tag="ACGI59_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFG9741449.1"
                     /translation="MSTLIKFELKKIIKRKSTIAVILGSLFLSIILFALMPIQEKAND
                     ESGKEYRGLQAIELKKQQSKLNEGYITDKKVKNSIRDYQKMFNNPNNITTDEGGFSNF
                     KPDIYWKFFYPNKAFYNFIIESYSNPNDYNPEIIKSMPLEDGVNFYKFRNEKVNTILN
                     MEYPDKNYTQKEKDFWLSKNKDIEEPYLNGYYQGWNYFLLARDLLIFTIVAVCISTAS
                     VFSGEYQSGADSIILSSKYGKTKVITAKIVSSFLFGTIIFTINTIVSIGILLLSFGID
                     GWNLPLQLTTSSIPYPYTFLQAILLSVLIIYMILILLISFTLLLSSYMKSPFPVLITN
                     IILLFVPIFLKYSETNKLYNQILNVLPSNAISFSFTNYVDYKFGNLIIALPTMIIFTC
                     LVLTVVFLPFIKNNFKKHQVN"
BASE COUNT         1531 a          395 c          557 g         1332 t
ORIGIN      
        1 attaattatg agtattaatt aatggtatag ataattttaa tataactgta tttataaata
       61 attataaaat aataaattaa tttttttata ctgacttttt tatagtataa atcgttatat
      121 ataatgacgg aggtgataat agtgaatgat gctcggatta tatcacaaat aaaagcagga
      181 aaaaaagaat tgtttgatac tctaataaaa aaatactatg atagcatcta ctgttattgt
      241 tatcgccatg tagacaataa aatgactgca caagatttaa ctcaagatgt atttgtaaca
      301 gttataaata atatagaaaa ttataaacat tatggtaagt ttgaaaacta tttgtatgtt
      361 attgcaaaaa acaagtgtaa ggatttttgg aagaaaaaac aaccaatata tttagagtca
      421 ttagataagg agatatcaaa tcaagaatta tctgatatag aaaataaaat gtttataaaa
      481 gaagctttga ctaaattacc agaattagaa tttgaagtga taattttacg gtattaccat
      541 gatttaagat ttaaggatat tgctaaaatt atggagtcaa gcgtctctgt cacaaagtac
      601 aggatgagta gggcattaaa aaaactagaa ttatatctgg aaggaggtaa attatgaata
      661 ataaagaaat aaaaagaagt ctacagaagt ataaggttcc caaatatgat tcttcagctc
      721 ttgaaaagac tattaaaact atacatgagt taacgcttag taatacttat aaaagaatga
      781 ataataaaga cttttttata tctcaaattg gttttataag aaaaagaact tggattatgc
      841 aatttctaat actatgtaca atatgtatat ttttatttag tacatcagca gatagtaatt
      901 cattttataa tagtttatcc ttgatttcta tagcatcacc actttttgta ctgacaaata
      961 tagaagaatt aacaaaagtg tataatagta gtattttaga gatagaattt tcaacaaaaa
     1021 atacattaca taaaattatt gctactagaa tgcttatatt tggaataagt gatttattgt
     1081 ttatgattat tattttggta tctgcaaaac aaatcattga tgtaaacatt ctgtatataa
     1141 taatttatat actagttcct tttaacctaa tgtgtatagg atgtatggaa attataaata
     1201 gaaataaagg taagcataca aattattatt gtatgatata cggtatatct cttgtattat
     1261 taatagttac actgagtaac tttagagata atctatatca atttgaagca ataaatcaat
     1321 ggctaattgt ttttctagtc acagcagtaa cattaatata tgaaataaga aaaacgtgga
     1381 gacatctttg ttcatttgaa aatattatta tctaagtaaa aaataagaat acacagtatc
     1441 aggaggaata gtatggagtt aagtgtagaa cgtttaacaa aacagtataa caataaaatt
     1501 atagtagata aaattgactt taaattaaat gagggtatat atggattttt aggagaaaat
     1561 ggagctggaa aaacaactct tatgagaatg atttgcggaa tattaagtcc tacatctgga
     1621 gaagtaaagg tggatggtaa aaataatttg gatatgggag aagattttag agatatgatt
     1681 ggataccttc ctcaaaactt tggatactac ccagatttta ctgccaaaga atttatgcta
     1741 tatatagcat cattaaaagg attaatgcct agctatgcaa agagaaaaac acaagaatta
     1801 ttagaaatgg taggattatc agaggtaatt aataaaaaaa taagaacatt ttctggaggt
     1861 atgaagcaaa ggctaggatt tgctcaaaca ctacttaatg accccaaaat tttaatatta
     1921 gatgagccaa cagcagggct agaccctaaa gaacgagtta gatttcgcaa cctaatttca
     1981 gaattttcta aggaaaagat tgttatattg tctactcata ttgtgtctga tgtagaatat
     2041 attgcagata aaattttaat aatgaaaaaa ggaagatttg ttttaactgg ctcaatagaa
     2101 gaagtaactt caaagataaa agataaggta tggaaatgtg tggtcaatga gaatgaaata
     2161 gattatatgt ctgatagata ttgtttagca aatatccatc attctggtgg agttgcaaat
     2221 cttagaattg taagtgatat aaagccacat gacaatgcat gcaatttaaa accaacatta
     2281 gaagatttat atttatattg tttcaacgaa gtactataaa tatacataag gagataggta
     2341 tgtctacatt aattaaattt gagttaaaaa aaataataaa aagaaagtct acaatagcag
     2401 ttattttagg aagtttattc ttaagcatca ttctttttgc attaatgcca attcaggaaa
     2461 aagctaatga tgaaagtgga aaagagtata ggggtttaca ggctattgag ctaaaaaagc
     2521 aacaatctaa attaaatgag ggatacataa cagataaaaa agtaaaaaac tcaatcagag
     2581 attaccaaaa aatgtttaat aatccaaata atattacaac agatgaaggt gggttttcaa
     2641 attttaaacc agatatttat tggaaattct tctatccaaa taaagctttt tataatttta
     2701 taatagagtc atatagtaat ccaaatgatt ataatccaga aataattaaa tcaatgcctt
     2761 tagaagatgg agttaatttt tataaattta gaaatgaaaa agtaaataca atattaaata
     2821 tggaatatcc tgataaaaat tacacacaga aagagaaaga tttttggttg tctaaaaata
     2881 aggatataga agagccttat ttaaatggat attatcaagg atggaattat tttctattgg
     2941 ctagagattt actaattttt actatagtag ctgtctgcat aagtacagct tctgtatttt
     3001 caggagaata ccaatccggt gctgattcaa taatattatc ttctaaatat ggaaaaacaa
     3061 aagttataac agctaaaata gtaagttcct ttttatttgg tacgattatt tttacaataa
     3121 atacaattgt gtctattggt atacttcttt tatcttttgg aattgatgga tggaatttac
     3181 cattacaact tacaaccagt tcaattccat acccatatac atttttacag gcaatattac
     3241 taagtgtgtt aattatttat atgatattaa tactattaat tagttttaca cttttattat
     3301 catcttatat gaaatcacct tttccagttt taattacaaa tataatatta ctatttgtac
     3361 caatattttt aaaatatagt gaaacaaata agttgtataa tcaaattctc aatgttttac
     3421 catcaaatgc tatttcattt tcatttacta attatgtaga ttataaattt ggtaatttaa
     3481 taatagcttt acctacaatg attattttta catgcttagt tcttacagtt gtttttttac
     3541 cttttattaa aaataatttt aaaaaacatc aagttaacta aaatatatat tataaaaaga
     3601 aggtgtctta aaatgaactt tttagcttat taggcactct tctttgtatt aatttaaata
     3661 tattttagta ttagattaaa tatgttttca tcattttaac aatgaaaaag agctttatct
     3721 ctattttgag atagctctta taaagttaaa tattaaattg ataactataa aatatgtagt
     3781 ttgaatttat aatttgtgtt ataatcatac tgatt
//
LOCUS       DAYAPA010000210         3714 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.215, whole genome shotgun sequence.
ACCESSION   DAYAPA010000210 DAYAPA010000000
VERSION     DAYAPA010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3714
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.215"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            253..1833
                     /locus_tag="ACGI59_003701"
     CDS             253..1833
                     /locus_tag="ACGI59_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HFG9741450.1"
                     /translation="MLIGTSKLSRNVDTACVEEVGIPMIVLMENAVISAMKNMDIDMY
                     NSYTVVCGVGNNGGDGLGIARHLNTYGKEVNVFLVGNLEKLSECSKINYNVLLNMGIN
                     IININKNDLSEKNYGEENEVDKIIKVEKQNIIKNKNLTIFKDRVSKSDVVVDAIFGTG
                     LKRDITGIFKEVIDIVNDNSKNTYSIDIPSGIDSDNGNVLGTCIRANKTISFEFYKRG
                     FLNYDTSALIGDVVVEKIGVPDFITRKYHDEEFITDKTFVKNNIRRRDKHGFKSDFGK
                     VSIVAGSKGFYGASFIATESAVKSGSGLVTLISSEDVQEKVSIRLTEAMTLNFEEERL
                     NKLLNSSDAIGFGPGMGDNSQTFDKLLKIVENSNCPIVLDADGLNVMKDRCYKFLEWK
                     NRFVITPHLGEMARLTGDSIGYIREHRVDVAKEFAQKYNLVVLLKGYQTVITDGKKTY
                     INPTGNSCMATGGMGDCLLGMITSFIGQGMDILEATVSGAYIHGYIGDKLSKAMYTVN
                     ATDLISNISLTMKELLDI"
     gene            2007..2453
                     /locus_tag="ACGI59_003702"
     CDS             2007..2453
                     /locus_tag="ACGI59_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFG9741451.1"
                     /translation="MKLIWLIVAVLFGIAEMLTPSLTLIWFSVGAVILIFLSSFIESI
                     FLQILIFAVISIAMLVVATKKIVKKDKGYKSNTNLQAMMSKKGIVTEEISPNNTGLVV
                     VEHETWTAISIDGEKIEKGSTVEVLKIEGVKLVVKKVDATVNVTNQ"
     gene            2474..3517
                     /locus_tag="ACGI59_003703"
     CDS             2474..3517
                     /locus_tag="ACGI59_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFG9741452.1"
                     /translation="MGTKIVLSIVLIVVVVAISLTCIRVIKQSKVGIIMRLGKFQKVA
                     ETGVHFLIPFLDKMAYVIDLREIVIDFPPQPVITKDNVTMQIDTVVYYKVTDPVRYVF
                     EIANPIAAIENLTATTLRNIIGELDLDETLTSRDIINVKMRTILDEATDKWGIKVNRV
                     ELKNIMPPQDIQVAMEKQMRAERERREAILQAEGNKSAAILQAEGEKQSAILTAEAKK
                     EAMVRVAEGEKESAILVAEGEAEAIRQTAIAKAQGEAEMIKRTQMATAEGLKLVFSAM
                     KEADIDNNILALKSMEALEKMAEGKSTKLVLPSEAVNFLGTFKGIKEVMSDDNKEVLD
                     IKEVLNDNESLKK"
BASE COUNT         1499 a          338 c          701 g         1176 t
ORIGIN      
        1 taaaagatta taaaaataaa gagatattta gataattagt gtaaataggt tttaaatcaa
       61 cataataact taaattatat ggtataataa attaaataat tgttttaatt caaaattttt
      121 tgttatttta aattatttca aaactcttag ttttagtgat tttatttatg atatttatgg
      181 gattaaaaag atttcaatag atatttttgg taagtattta agaagtcttt aactttatag
      241 gagtgataat ttatgctaat aggtacatct aaattaagca gaaatgttga tactgcatgt
      301 gtagaagaag tagggattcc aatgattgta ttgatggaaa atgcggttat atctgcaatg
      361 aaaaatatgg atatagacat gtataatagt tatacagttg tatgtggtgt tggaaataat
      421 ggaggagatg gtcttggaat agcaagacat ctaaatacat atggaaaaga agtaaacgta
      481 tttttagtag gtaatcttga aaaactaagt gaatgctcta aaataaatta caatgtactt
      541 ttaaatatgg gaattaatat tataaatatt aataaaaatg atttaagtga aaaaaattat
      601 ggagaggaaa atgaagtaga taaaattatt aaagttgaga aacaaaatat tataaaaaat
      661 aaaaatttaa caatttttaa agatagagta tctaaaagtg atgttgtagt agatgctata
      721 tttgggactg gattaaaacg tgatataact ggtattttta aagaagttat tgacatagta
      781 aacgataata gcaaaaatac gtattctata gatattcctt caggtataga tagcgataat
      841 ggaaatgtat taggaacttg tattagagca aacaaaacga ttagctttga gttttataaa
      901 agaggttttc taaactatga cactagcgca cttataggtg atgtagttgt agaaaagata
      961 ggtgttccag attttattac aagaaaatac catgatgaag agttcataac tgataagact
     1021 tttgtaaaaa ataatattag aagaagagat aagcatggtt ttaaaagtga ttttggaaaa
     1081 gtatctatag ttgcaggttc aaaaggattt tatggtgcaa gtttcatagc aactgaatct
     1141 gctgttaaga gtgggagtgg acttgtaaca ttaattagta gtgaagatgt gcaagaaaag
     1201 gtatctatta gacttacaga agcaatgact ttaaattttg aagaagaaag attaaataaa
     1261 ttacttaatt ctagtgatgc aattggtttt ggtccaggta tgggagataa tagtcaaaca
     1321 tttgataaat tacttaagat agtagaaaat tctaattgcc caatagtatt agatgcagat
     1381 ggattgaacg ttatgaagga cagatgttat aagtttttag aatggaaaaa tagatttgta
     1441 attacacctc atttgggaga aatggccagg ttgacaggag attcaattgg gtatataaga
     1501 gaacatagag tagatgttgc aaaagaattt gctcaaaaat ataatctggt ggtgctttta
     1561 aaaggttatc aaactgtcat aacagatggt aaaaaaacat atataaatcc tacaggaaat
     1621 agctgtatgg caactggtgg aatgggtgat tgtttgcttg gaatgattac ttcatttata
     1681 ggtcaaggta tggatatatt ggaagctaca gttagtggag catatataca tggctatata
     1741 ggagataaat tgagcaaagc aatgtatacg gtaaatgcta cggatttaat aagtaacatt
     1801 tcattaacaa tgaaagagtt gttggatata taattggtga tatttgatac tactttgtaa
     1861 caattttaac aaagtatgtt gatataatac tattaattaa ttaatttatt ttattgtttt
     1921 gtaatttaaa tttatattca aaaacattgt ataaacatat aatttatata gggttttata
     1981 attcatatga aaagagggta gatagtatga agctaatatg gttgatagta gcagtcttat
     2041 ttggaatagc agaaatgtta acaccaagct taacattgat atggtttagt gtaggagctg
     2101 taatacttat atttttaagt agctttatag agagtatttt cttacaaatt ttaatatttg
     2161 cagtaatttc tatagcaatg ttagtcgtag ctactaaaaa aatagtaaaa aaagataaag
     2221 gctataaatc taacactaat ttacaagcaa tgatgtctaa aaaaggtatt gttacagaag
     2281 agatttctcc aaataacaca ggtctagttg tagttgaaca tgaaacatgg acggctattt
     2341 ctatagatgg tgaaaagata gaaaaaggtt ctacagtaga agtattaaaa attgaagggg
     2401 taaaattagt tgttaaaaaa gtagacgcca cagttaatgt gacaaaccaa tagaataaag
     2461 gaggatttat agtatgggta ctaaaattgt tttaagtatt gtattaatag tagtagtcgt
     2521 agcaataagt ttaacttgta taagagttat aaaacaatct aaagtaggta taataatgag
     2581 acttggtaag tttcaaaaag tggctgaaac aggtgtacat ttcttaatac catttttaga
     2641 taaaatggca tatgtaattg accttagaga aattgtaata gattttccac ctcaaccagt
     2701 tataactaaa gataatgtaa ctatgcagat tgatacagtt gtatattata aagttacaga
     2761 cccagttaga tatgtgtttg agatagctaa tccaatagct gctattgaaa atttaacagc
     2821 tacaacacta agaaatataa ttggtgaact tgatttagat gaaacattaa catcaagaga
     2881 tataataaat gtaaaaatga gaactattct tgatgaagca acagataaat ggggtataaa
     2941 agtaaataga gtagagttaa aaaatataat gcctcctcag gatattcaag ttgcaatgga
     3001 aaaacaaatg agagcagaaa gagaaagaag agaggcaata cttcaagcag aaggtaataa
     3061 gtcagctgca atacttcaag ctgaaggtga aaaacaatct gctatattaa cagcagaagc
     3121 caaaaaagaa gctatggtac gtgtagcaga aggtgaaaaa gaatctgcta tattagtggc
     3181 agaaggtgaa gctgaagcta taagacagac agctattgct aaggcacaag gtgaagctga
     3241 aatgataaaa agaactcaaa tggcaacagc agaaggttta aaattagttt tttcagcaat
     3301 gaaagaggct gatatagaca ataatattct agcattaaaa tctatggaag ctcttgaaaa
     3361 aatggctgaa ggtaagtcaa caaaactggt tttaccttca gaagcagtta atttcctagg
     3421 aacattcaaa ggaataaagg aagttatgag tgatgataac aaagaagtac ttgatataaa
     3481 agaagtttta aatgataatg aatcattaaa aaaataaaag agttttgtta tcataggaag
     3541 gatatggaaa ttaaaggtcg aattaattaa atatagcaca aaaatgcacg ttgaaaagaa
     3601 gaggacttat aaatttttaa gtcttcttct ttgtttttat tagaaagatg aaattataaa
     3661 aataaattaa cagaattttt atacaattta tcctttacta atgtaaaaag tgtt
//
LOCUS       DAYAPA010000211         3676 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.216, whole genome shotgun sequence.
ACCESSION   DAYAPA010000211 DAYAPA010000000
VERSION     DAYAPA010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3676
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.216"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            266..1642
                     /locus_tag="ACGI59_003704"
     CDS             266..1642
                     /locus_tag="ACGI59_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HFG9741453.1"
                     /translation="MKTIVRRLILYADGMRPTQIMVSGFAAIIVIGALLLTLPIASQS
                     GESIGLLNALFTATSAVCVTGLVMVDTATYWSLFGQIVIITLIQIGGLGFMTVATMFS
                     LMARKKIQLRERLLIQESLNQADLSGLVRLTRFVLIITITIEGIGALVLSTVFIPQFG
                     LSKGIWYSVFHAISAFCNAGFDLMGSVSGPFTSLNSYVNNFTVSMTVCALIVLGGLGF
                     PVVLDIVRKRRFSKLNVHSKVVLFSTATLIFVGALFIFLIEFNQKATMADLPLKGKVL
                     SAIFQSVTARTAGFNTLDLATLRESSVFVMIILMFIGASPASTGGGIKTTTLAVLIIT
                     VRSFLSGKSDIEAFERRLAPSTIKKSLGIFVISISAVIFGTLIISITQPNFTLVQSAF
                     EVTSALATVGSSLAGTPNLNALGKIIIIIFMFMGRVGSLTLFMAILSGGRRKSQPIRY
                     AEGKIMVG"
     gene            1953..2624
                     /locus_tag="ACGI59_003705"
     CDS             1953..2624
                     /locus_tag="ACGI59_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HFG9741454.1"
                     /translation="MKQYIVIGCGRFGSSVASTMHLLGHQVMAIDKNEDSVQSISDKV
                     THSLIVDVTDEQALRSLGLGNFDVAVVAIGSDIRASIMATLIAKEMGVELIICKAKDE
                     LQAKVLYKIGADRVVFPERDMGVRVAHNLVSDNILDHIELDPEYSIVEIVTPNSWVGK
                     TLIELELRARYEITVLAIKTGKNINVTPSPDEELTAGSILVIIGQNTSITAITSGNKG
                     IIRRR"
     gene            2813..3610
                     /locus_tag="ACGI59_003706"
     CDS             2813..3610
                     /locus_tag="ACGI59_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HFG9741455.1"
                     /translation="MITNINSKDNEKLKYTRALLKSKNRNKESKFIIEGYRIVMLALE
                     CMANLDYVFINEEFENKKEHVKLLEDLDKKNTKIYKTTNKNFKELVDTENTQGIIGVV
                     SFKKKKLSESINKKDKFVLILDRIQDPGNMGTIIRTADSAGVDAIIALKGCVDIYNPK
                     VIRSTMGSIFDMNIIDASQDETVDMLKSLDFNIVSSYLNTENFYDKIDYGSKVALVIG
                     NEANGINEELVSKSDILVKIPIYGKAESLNAAISSAILMYEIKKYLI"
BASE COUNT         1390 a          409 c          683 g         1194 t
ORIGIN      
        1 ataaatttta agtacaaaat agtaaaagct atgatagttt tagacctatt atatgtaaaa
       61 aatatagatg tttttggtgc aatttaaatt aaaagatata tataaaaatt taaaatattc
      121 caaacagaaa ttataaaaat tactatcatt gtctttatac aatatttata ctataataga
      181 ataataattt agaggaaagc tctaaaaata aacaaattaa gggtcttaac agtagtgatt
      241 ttatgttaag gaggtataaa gaaagttgaa aacaatagtt agaagactta ttctctacgc
      301 agatggaatg cgtcctactc aaataatggt atctgggttt gctgcgatta ttgtaatcgg
      361 agcattgttg ttaacactcc cgatagcgtc gcagagtgga gagagtatag gcttattaaa
      421 tgctctattt acggctactt cagcggtttg tgttactggt ctagttatgg tagatacagc
      481 aacttattgg agtttatttg gacagatagt tataataaca ttgatacaaa ttggtggtct
      541 tggatttatg accgtagcca caatgttttc acttatggca cgaaaaaaaa tacaactaag
      601 agaaagatta ttaatacaag agtcattaaa tcaagctgat ttatcgggac ttgtaagact
      661 tacaagattt gtattaataa taaccataac aatagaggga ataggtgcac tggttttatc
      721 aactgtcttt attccacaat ttggtttgtc taaagggata tggtatagtg tatttcacgc
      781 aatatcagcg ttttgtaatg ccggatttga cttgatgggc agtgtaagtg gcccatttac
      841 atcgttaaat tcttatgtaa acaactttac agtatcaatg actgtttgcg cacttatagt
      901 gcttggaggg ctaggattcc ctgttgtact tgatattgta agaaaaagaa gattttcaaa
      961 attaaatgtg cattctaagg ttgtattgtt ttcaactgct acacttattt ttgtgggtgc
     1021 attatttatt tttcttatag aatttaatca aaaagctact atggcggact tgcctttaaa
     1081 gggaaaagta ctatcagcaa tatttcaatc agtaacagca agaactgctg gttttaatac
     1141 gcttgattta gctactttac gagaaagtag tgtatttgta atgataattt tgatgtttat
     1201 tggagcatca cctgcatcaa ctggtggggg tataaaaaca acaacactag ctgttttaat
     1261 cattacagtt agaagttttc tatcaggaaa atcagatata gaagcttttg aaagaagatt
     1321 agctccatca actataaaaa aatctcttgg tatatttgta atcagtatat cagcagttat
     1381 ttttggaaca ttgattattt caataactca gcctaatttt acattagttc aatcagcttt
     1441 tgaggttaca tcagcacttg ctacagttgg ttcaagttta gctggaactc caaacttaaa
     1501 tgctttaggt aagattataa taataatctt tatgtttatg ggtagagttg gttctcttac
     1561 tttatttatg gctatacttt ctggtggtag aagaaagagt caaccaatca gatatgcaga
     1621 aggtaaaatt atggttggtt aagttaccaa ggagtagtat aaatttataa tgggtttaga
     1681 gtagcactgt aaatctgttg ataaaataaa tgagcctaga tgtaattata tgaagaaata
     1741 aaatcaacta atgaacaaaa ttaattaatg aaataaaaaa acagggaaat aaaatacaaa
     1801 taaaaaggta aatcaaaatt aaattaatct gtgtgtataa aattatatat tgtgtataag
     1861 ttttgtatgt gtaaagtata taagttttgt atgtgtaaac aattaattta agagatatta
     1921 tataaattgt gtattaggga ggaagtttcg agatgaagca atatatagtc attgggtgtg
     1981 ggagatttgg aagttcagtt gcgtctacta tgcatctttt aggacatcaa gtaatggcaa
     2041 tagacaaaaa tgaagattca gttcaaagta tatctgacaa ggtaacccat tcacttatag
     2101 tggatgttac tgatgagcaa gcgttaaggt cattaggttt aggtaacttt gatgtagcag
     2161 tagttgcaat aggttctgat ataagggcat ctataatggc gactcttata gccaaagaaa
     2221 tgggtgtaga gttgataata tgtaaggcaa aggatgaatt acaagctaaa gttctttata
     2281 aaattggtgc agatagagtt gtatttccag aaagagatat gggagtaaga gttgcacaca
     2341 atttagtttc ggataatata ttagaccata ttgaacttga cccagagtat tcaattgttg
     2401 aaatcgtaac tccaaatagt tgggttggca agacacttat agagcttgaa ttaagagcta
     2461 gatatgagat aactgtactt gctataaaaa caggtaaaaa tataaatgtt acaccttctc
     2521 cagatgagga acttacagcc ggaagtatcc tagttataat cggtcaaaat actagtataa
     2581 cagcgataac atctggaaat aaggggataa ttagaagaag ataatttact atttaatata
     2641 tatttaattg caatgaaagc agatagtatc atataattat gaatagttat atgatactat
     2701 ttttttaatc gaaggtagta tttatttgta agattagata aagagaagtt aaattaaaag
     2761 taaggaggct gtgctaatat aaaaatttat aagttattag catttagata aaatgattac
     2821 aaatataaac agtaaggata atgaaaagtt aaagtataca agagcactat taaaatcaaa
     2881 gaataggaat aaagagtcaa agttcataat agaaggatac agaatagtaa tgcttgcact
     2941 tgaatgtatg gcaaaccttg attatgtatt tatcaatgaa gaatttgaaa ataagaaaga
     3001 acatgtaaaa ctattagaag atttggataa aaaaaacaca aagatataca agactactaa
     3061 taaaaacttt aaagaattag tggatacaga aaatactcaa ggaataatag gtgtagtttc
     3121 atttaagaaa aaaaaattaa gtgaaagtat aaataaaaaa gataaatttg tattgatttt
     3181 agacagaata caagacccag gaaatatggg gactataata aggactgctg attctgctgg
     3241 agtagatgcc ataatagcac taaagggatg tgtcgatata tacaacccaa aagtaattag
     3301 gtctactatg ggttctattt ttgatatgaa tataattgat gcttcacaag atgaaactgt
     3361 ggacatgctt aaatcattgg attttaatat agtttcaagt tacttaaata cagaaaattt
     3421 ttatgacaaa atagattatg gctcaaaagt agcattggtg ataggaaacg aagcaaatgg
     3481 aataaatgaa gaacttgtat caaagtctga tattttggtt aagataccta tatatggtaa
     3541 agccgagtcg ttaaatgctg cgataagctc tgctatactg atgtatgaaa taaaaaaata
     3601 cttaatttaa tgtattgtaa taaatataat gtcatgttat aatcttgaat taaatagtag
     3661 agtatcaaaa atagtc
//
LOCUS       DAYAPA010000212         3562 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.217, whole genome shotgun sequence.
ACCESSION   DAYAPA010000212 DAYAPA010000000
VERSION     DAYAPA010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3562
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.217"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(144..1711)
                     /locus_tag="ACGI59_003707"
                     /pseudo
     CDS             complement(144..1711)
                     /locus_tag="ACGI59_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021426623.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iota toxin protein Ib"
     gene            complement(1704..2229)
                     /locus_tag="ACGI59_003708"
                     /pseudo
     CDS             complement(1704..2229)
                     /locus_tag="ACGI59_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890822.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iota toxin protein Ia"
     gene            complement(2618..3364)
                     /gene="cdtR"
                     /locus_tag="ACGI59_003709"
     CDS             complement(2618..3364)
                     /gene="cdtR"
                     /locus_tag="ACGI59_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family response regulator CdtR"
                     /protein_id="HFG9741456.1"
                     /translation="MDILIFDNDVCFGMKLKEKINNILIKEGFDNDVVRLYHNANLLL
                     KELTEKNKVRIYFIVVDAKYKISNELCDGLWIAQKIRESDYISPIIFLTNHIEMILGI
                     FDYRLEVMDFILKHDMEIAESKIKACIKIAHKRYIKEKNYRSNFFTIYSDFSLWKISF
                     DEVIYFETSTIPHKIKLVTTSRIFEFYKSLKSLPDLDACFIRVHKSFVVNKYHIVSLD
                     LKKNNIKMSNGHICRISNTYRNILKNIIKT"
BASE COUNT         1201 a          457 c          383 g         1521 t
ORIGIN      
        1 tttttaatac aatatagttc taaaaaagaa tatattagaa gaagataata tttaaataaa
       61 aaatacctta taaagttaat tttttaatac tttataaggt aaatacatat tgtattgtgg
      121 ggataaattt aaatcatttg atactaatca acactaagaa ccaataactc tctatcatct
      181 tgagtaattg tatatattct taatttcttg tatgtcataa tattttctcc acctgtaaaa
      241 taactcctaa gattaacaca atcagtttta ggttgatttg tatctcctaa ataattacgc
      301 aattctttat ctttagtttc aatatcttta aatccaaaat attgtaaagt agcttcaact
      361 ctatattttt ggacataatc gagagcttct ttattagttt atgttggtgt aaaatacata
      421 ccatttatat aagcctttat tgtacttgga ttaaatttaa aatctgcaga atatatttta
      481 tgtgtaacta ttatttcttg aacagttgga attttaactt ctggttcatc aagtatttca
      541 ggagtactat ttagctctgt aatagataag ttatctaagt atattgtatc actatcaatt
      601 aatgctatat ttatattaga agaatctttt tcagtagttt caaattcata actaaatttt
      661 gtatatcctt gttctagtac caaataatcc gttttttctt cttttacttt tatatttaca
      721 attattgaat tatatgttaa aggatttttt gaattttact aaaaacataa cgtttataag
      781 gttttagttc agacataggt attttaatct tcgtctcacc atttaatcta tttgctgagc
      841 cttttaaacc atcttgattt gtagtattaa cattactcca tatactaggg taattattgt
      901 aatcatcaaa attagtaaag taagttggtg tttttataag tatattcatt cctctttcaa
      961 gtttaacatt acatatttgt ttatcagaca aagtttttaa actgtcttta atcttattgc
     1021 ctatattatc atcaaatatg agttcaacac aactttcatc tattggtata ttattaaaat
     1081 ataacaaacc atctttttta gtagtgccaa aagttttttc aattgcttct ccaattgtaa
     1141 gttcaggtgt tttatcttct gggacctgta aacttttagc agtaattctt cttttgtaag
     1201 attcattttc tgtatctaat ataatagata cagaaatact gtcaatttga cctatatcgt
     1261 cttaccaact atttcattct gttactattt gtccagaacc attttttgta ccaaaatttc
     1321 cacttacttg tgttgtttct aatttaattt gctttccatc atctaatttt tttaattaat
     1381 tataatttat aggaatcagt ctagaactaa attgatacat tgtgttaaga gccataggtg
     1441 aaaatccttt tttgagataa gtatttccag gagatagatt attgccaatt tgattttctt
     1501 gtgctttgat agttgataat gcatctccat ctaacactaa atttgttgtt ggtgtcactt
     1561 tgtacatagg tgcagtacct gtgttgtagt atctaatatt tgcatttata tatgcagatt
     1621 ctcctttgtt tatacttaat ccagtatttc atgattatcc attgctattt tgaacagcag
     1681 ttgaattatc tgttgtatgg taataatttg tcttgtatag tttctttaaa ctcattaaat
     1741 ttttctaatg aaatttcgtt ttgatttttt gttcttatta ctttattaaa tgcaaatttt
     1801 tctggagatt caaaataata tacatacata ggtttatcta ttttattttt tgatatagca
     1861 tttcgaagtt ctctatattc tttttctcaa gaatttgctt ctatttgata atcataaaaa
     1921 taatttcttg tctgagaata tttgcttatc tctatagaat cttttttata tgatcctaat
     1981 acttcttttt cagatttttc aagtttttga tcttttcttt ctcattcttt agccttttct
     2041 ttatctttaa aaaaatcctt ctgatctttc tataggagcc ttgtaagtag tattacaaac
     2101 ttttccacta tagctttgta aatcttgtgc ataaatgtta ttaggtaaca aactacctaa
     2161 agttagatat aatatcaaca atatagatat acaattacta atacttttat gtttcctaaa
     2221 ttttttcatt tattctccct acaaatatta gtttagttaa cttttgaatt ttaatttatt
     2281 tagattatat aatctaaata agaatttttt atataaattt cataaaatat gtaggtatat
     2341 atcataaaaa tatgtattta tatgatatgt cagtacaaaa taacaactga atattttaaa
     2401 gagactattt cgtactttta tgatttaatg agctatctta tcgaatcata atctaggaac
     2461 aattgttata atatctaata gtagttatat atgatatagt cagtatgtgt tttttcacaa
     2521 tatgattatt atatagagga ttcttaggga ttactctttt tgtataaaaa agagatggtt
     2581 caaattttta tcttgagaca tctccttttt ctatttatta tgttttaata atattcttta
     2641 aaatatttct atatgtatta gatatacgac atatatggcc attactcatt ttaatattat
     2701 ttttctttag gtcgagagaa acaatatgat atttattaac tacaaaagat ttatgtacac
     2761 ggataaagca tgcatctaaa tctggtaaag actttaaact tttatagaat tcaaatattc
     2821 tagaagtagt cactaatttt attttatgtg gaatagtgct tgtttcaaaa tatataactt
     2881 catcaaatga aattttccat agtgagaaat ctgaatagat agtaaaaaaa ttagagcgat
     2941 aatttttttc tttaatgtat cttttgtgag caattttaat acaagctttt attttacttt
     3001 cagcaatttc catatcatgt tttagtataa agtccataac ttctaaccta taatcgaaga
     3061 tccctaaaat catttctata tgatttgtaa gaaatatgat tggactaata taatctgatt
     3121 ctctaatttt ttgtgctatc cataatccat cacataattc attacttatc ttatattttg
     3181 cgtctacaac tataaaatat attcttactt tatttttttc agtaagctct tttaataaca
     3241 aatttgcatt atgatataat cttacaacat cattatcaaa accttctttt attaaaatat
     3301 tatttatttt ttcttttagt ttcattccaa aacaaacatc attatcaaag attaatatat
     3361 ccactaaata ccctcctata aaaaattcaa aagtaaaaaa acaaaagtat tttatagaaa
     3421 atacataaaa taacactagg agataaataa gattaaggat agttagaatt aaggtatatt
     3481 tatgcttgtt tatatagtat atcttatact tatagtttac aataataaag taaattaaat
     3541 caatatatta ttataaaaaa tt
//
LOCUS       DAYAPA010000213         3508 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.218, whole genome shotgun sequence.
ACCESSION   DAYAPA010000213 DAYAPA010000000
VERSION     DAYAPA010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3508
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.218"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(387..608)
                     /locus_tag="ACGI59_003710"
     CDS             complement(387..608)
                     /locus_tag="ACGI59_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1659 domain-containing protein"
                     /protein_id="HFG9741457.1"
                     /translation="MEVVSTKNLSSIKLKLDAGFDDKGKAVVKSKSFANVKAEALNED
                     VYAVAEAIASLQENPIVDILKLDSTSLSK"
     gene            1188..1445
                     /locus_tag="ACGI59_003711"
     CDS             1188..1445
                     /locus_tag="ACGI59_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mor transcription activator family protein"
                     /protein_id="HFG9741458.1"
                     /translation="MKIKVEDIPVQFHAMVEIIGIDKFIEVAKLYGGTNTYIPTYKGL
                     FRYARNRDIVKQFNGVNHNELAIRYNMCVSNIKRILNENSV"
     gene            1914..3152
                     /locus_tag="ACGI59_003712"
     CDS             1914..3152
                     /locus_tag="ACGI59_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conserved phage C-terminal domain-containing
                     protein"
                     /protein_id="HFG9741459.1"
                     /translation="MNKGFYKLNSVVLNFDIKNDVNKIRFNHYIMRKENIQRLQGTLP
                     NGQFYMTVRRAATDLQLSISTISRLVKEFLDLGIIRLVSKGVKGNRSVYSYVSIEFNG
                     SLFDDSKYMELDCVCNDEFIDNSGIEFDCDSKDNSFNGDINVETVHCINEYFSDDDVE
                     HNYNNFHNKDTIKDKAISNISTIKKYIDESFDTNKSENSNLQQESINFNFNDTCNEID
                     KRTNNSIENNQNKRPMRLSDISLLSDKGMTNKKNYFNHNHGIQKGTKSVTKKKESLNK
                     FNKKNIQQSIINMLNKKSGKNFKIDSPISIKLINERLKEGYVLEDFYKVIEVKVAKWK
                     NTIMEMYIRPETLFSYKFESYVNENISMKSQSNVHPCKEGNSNDSSNYIHSDYITYDG
                     SVRERKKYENVRYWNPICEI"
BASE COUNT         1437 a          312 c          528 g         1231 t
ORIGIN      
        1 tagtatttca attgtcaata ttaatgtttt gcttgttagt attagtattt caattgtcaa
       61 tattagtgtt ttgcttgtta gtatcagtgt ttcaattgtc aatattagta ttttaattgt
      121 tgactctagt tattgctgat atagaaaata tttaatttat ttcaattaat tagattaaag
      181 tagggtagca tctaaaatag ctaacatgtt taagttgaaa tacgtttaat atattaattt
      241 ttcaaagcgt taatatatta aagttataac caaaattgaa gttataatct gccaaatcta
      301 tagcgtatta agtgcattat ataacttaat cttttatata aagagttaat tttttaattt
      361 gtaaagcaga gtacgtttaa aagacactat ttagatagtg atgtactatc taattttaaa
      421 atatctacaa tagggttctc ttgaagactt gctattgctt ctgcaacagc ataaacatct
      481 tcatttaatg cctctgcttt tacatttgca aatgatttac ttttaacaac tgccttaccc
      541 ttatcatcaa aaccagcatc caattttagt tttatactag ataaattttt tgttgaaaca
      601 acttccataa aattacctcc taaaaattta atttcaaaga tttgtcgact ttctttgtta
      661 gctataatac tatcatctaa gataatatcg gagttaaaca gtaatttcta aagatttttt
      721 gtgtaaattt ttataggaaa aattttactt ttttgctgat gaaaaagttt acaagttatt
      781 tgtgaagata tgttcttagt gttttgaaat agtatttgta tatattcatg cttagttgag
      841 atagttttgt atggattttc atatgtgtag agttaaatgt taggtactaa agtttatgtt
      901 aaattaaatg ttaaagctta tgttaaaaat tttattaaat ttaatctgta aataaaaaag
      961 ttaaaagtcc aatattaaca ttttactttt aaaccattaa aatctttatt aaagctaaca
     1021 aagttataag ttatgagaaa ttttattgaa gttaatacaa aaattaattt taaagctaag
     1081 aaatttttta ttagattcaa taaaactaat aaatatagtt tagtcaataa tgtattaaat
     1141 aaaaactttg ttaagtctat aaaaatatct taaaggaggt gtaacttatg aagataaaag
     1201 tggaggatat accagttcaa tttcatgcaa tggtagaaat aatcggaatt gataagttca
     1261 tagaggttgc caagttgtat ggaggaacaa atacttatat acctacgtat aaggggcttt
     1321 ttagatatgc tagaaatagg gatatagtaa aacaatttaa tggagtcaac cacaatgaat
     1381 tggctattag atataatatg tgtgtaagca atattaagcg aatactcaat gaaaatagtg
     1441 tttaaatata ataaaataat gtttaaacat aatgaaaata gccttgttaa acgtaagagt
     1501 aaagacaaag aagatgtttt ataaagtttt tttagacaaa ttaactaaat ttactactta
     1561 ttaatttaaa tacattgtat attctataaa aaaatttgtt aaatactgga gatatattga
     1621 gaatacaaag aaaatggtcg aaaatatttg ttggctaaaa aatagataaa atgatacaat
     1681 tgcttgtata aaggggaaaa ataaataaat aaactatata aataaagaaa tagcaattaa
     1741 tatgacataa tatatttata aaattaagaa tataaataga tattttaagg gattagttgt
     1801 taaattagga aatattaaaa aaaatatata tttatattta aactattttg ttatttatat
     1861 aaaaatactt atctagttac tagcaattat tagtagaaat gggggaaata gagatgaata
     1921 agggatttta caaattaaat tcagtggtat tgaactttga tattaagaat gatgttaaca
     1981 agataagatt taatcattat ataatgagaa aagaaaacat acaaagactt caaggaactc
     2041 tgccaaacgg acaattttat atgacagtga gaagagctgc tacagattta caactttcta
     2101 tttctactat aagtaggctt gtaaaagagt ttttggattt agggataata agattagtgt
     2161 ctaagggtgt aaaaggtaat cgttctgtat atagttacgt aagtatagag tttaatggaa
     2221 gtttatttga tgatagtaaa tatatggaac ttgactgtgt atgtaatgat gaatttattg
     2281 ataatagtgg catagagttt gattgtgata gcaaagataa ttcttttaat ggagatataa
     2341 atgtagaaac tgtgcattgc attaatgagt atttctctga tgatgatgta gagcataatt
     2401 ataacaattt ccataacaaa gatactataa aggataaagc catttctaat attagcacta
     2461 tcaaaaaata tattgatgaa tcttttgata caaataaatc agaaaattct aacttacaac
     2521 aggagtcaat aaatttcaac tttaatgata catgcaatga aatagataaa agaacgaata
     2581 atagtataga aaataatcaa aataaaaggc ctatgagatt gagtgacatc agtcttttaa
     2641 gtgataaagg tatgacaaat aaaaagaatt actttaatca taatcatggt atacaaaaag
     2701 ggactaaatc tgtaacaaag aaaaaagaat ccttaaataa atttaataaa aaaaatatac
     2761 agcaaagcat tataaatatg ctaaataaaa aatcaggtaa gaattttaaa atcgattctc
     2821 caataagcat aaaacttatc aatgaaaggc taaaggaagg ctatgtctta gaagatttct
     2881 acaaagttat tgaggtaaaa gtggctaagt ggaaaaatac cataatggaa atgtatataa
     2941 gaccagaaac tttgtttagc tataagtttg aatcctatgt aaatgaaaac atatctatga
     3001 aaagtcaaag taatgttcac ccatgtaaag aaggcaatag taatgatagc agtaattata
     3061 tacattctga ttatataaca tatgatggca gtgtgcgtga gagaaaaaaa tatgagaatg
     3121 taagatactg gaatcctata tgtgaaattt aattagttta aaaatgtatt tatgataaat
     3181 aagatattaa tagagaatag tgagctatag caaatgatat atcaatagtt ggtagtaagt
     3241 tatattaagt aagatattac tatcaggtag tgaattatat tgaacgatat attaataatt
     3301 ggcagtaagt tatatcaaat aagatattac tatagagtaa tgaattatat tgaacgatat
     3361 attaataatt ggcagtaaat tatattaaat aagatattac tatagagtaa tgaattatat
     3421 tggacgatat atcaatagtt ggtagtaagt tatattaagc aagatattac tatcaggtag
     3481 tgaattatat tgaacgatat atcaataa
//
LOCUS       DAYAPA010000214         3434 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.219, whole genome shotgun sequence.
ACCESSION   DAYAPA010000214 DAYAPA010000000
VERSION     DAYAPA010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3434
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.219"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            516..3158
                     /gene="adhE"
                     /locus_tag="ACGI59_003713"
                     /gene_synonym="adhC"
     CDS             516..3158
                     /gene="adhE"
                     /locus_tag="ACGI59_003713"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFG9741460.1"
                     /translation="MEKKEKVVEKSNVEVCSPEIVNSVETLRMRLEEIRLAQKEFATF
                     TQEQVDKIFLAASTAANQQRIPLAKMAVEETGMGIVEDKVIKNHFASEYIYNAYKDTK
                     TCGVIEKDEAFGFTRIAEPVGVLSAVIPTTNPTSTAIFKSLIALKTRNGIIFSPHPRA
                     KNCTIEAARVVHDAAVKAGAPKGLIGWVDVPSIELTNVVMAEADLILATGGPGMVKSA
                     YSSGKPAVGVGPGNVPAIIDESADIKMAVSSILVSKSFDNGMICASEQAVIVPEKIYE
                     EVKKEFKYRGAHFLNKEETEKVGKVVIIDGSLNARIVGQPAHVIAKMADVEVPKTARI
                     IIGEVESVELNEPFAHEKLSPVLAMYKSKSFEDAVAKAEKLVADGGYGHTSSLYADSI
                     NHPERVEKFVNAMKTCRVLVNTPSSQGGIGDLYNFKLAPSLTLGCGSWGGNSVSENVG
                     VKHLLNIKTVAERRENMLWFRAPEKVYFKKGCLGVAAREFKDVMDKKKAFIVTDSFLY
                     NNGYTKKLTDLLDEMGIKHTTFFDVAPDPTLACAREGAKAMADFQPDLIIAVGGGSAM
                     DAGKIMWVMYEHPEVDFQDLAMRFMDIRKRVYVFPKMGEKAYFAAIPTSAGTGSEVTP
                     FAVITDQDSGVKYPLADYELMPNMAIIDADMMMEMPPRLTAASGVDALTHALEAYVSM
                     LRTEPADGLALQAGKIIFEYLPRAYKNGKNDKEAREKMAMASTMAGMSFANAFLGICH
                     SLAHKLGAFHHVQHGVANALLINEVIKFNCAEAPNKMGAFSQYRYPDCIQRYAEFASF
                     AGIKGSTDQEKVDNLIKAIDELKAKVGLPKTIKEAGVEESKFLERLDAMVEQAFDDQC
                     TGANPRYPLMSELKEIYLKVYYGK"
BASE COUNT         1346 a          422 c          635 g         1031 t
ORIGIN      
        1 aaagcataat tattacatat tgacttaaaa ataacttata gagattagaa taattttagt
       61 ttatatattt tttaaaacta tacagttgtt ataaaaagaa aaattgcata caagtcaaaa
      121 ataagtgtta ttttataaaa atacgacaaa aattttattt actacttata caaataacat
      181 gcaaataaca tataaaattt aataagtaaa aaaaatgaaa gatataaatt atcattttag
      241 tattttattt agtcgaagta aattattcaa atgaaatata tctcatttag ataaaaaatt
      301 ttagtaaaag taaaaaaaat aatatatttg ttaaaaaata aattgacaaa atattcagaa
      361 aaaaacttct tgacctaaga gagtaaaata aataaaatct aaggtataga gatgaaatct
      421 ataaaattat tttaagtata agttaaaaat atagatgatg catttaagat ttataaaaat
      481 tagagatgca taattatttt agggaggttt ttagtatgga gaaaaaagaa aaggtagtag
      541 aaaaatcaaa tgttgaagta tgtagtcctg aaattgtcaa cagtgttgaa acattaagaa
      601 tgagattaga agaaattaga ttggcacaaa aagaatttgc aacatttacg caagaacagg
      661 tagataagat tttcttagct gcttcaactg cagctaatca acaaagaata cctctagcaa
      721 aaatggcagt ggaagagact ggtatgggta ttgttgaaga taaggttata aaaaaccatt
      781 ttgcctcaga atatatctat aatgcttata aagatactaa aacttgtggt gtcatagaaa
      841 aagatgaagc atttggtttt acaagaattg ctgagccagt aggtgtttta tcagcagtta
      901 taccaacaac taatcctaca tcaactgcaa tattcaaatc cctaatagca ctaaaaacta
      961 gaaatggtat aattttctct cctcatccaa gagctaaaaa ctgtactata gaagcagcta
     1021 gagttgtaca tgatgcagca gttaaggcag gagcaccaaa aggacttata ggttgggtag
     1081 atgttccttc gattgaatta actaatgttg tcatggcaga ggcagacctt atattagcta
     1141 ctggtggacc tggaatggtt aaatctgcat actcttcagg taaaccagct gtcggagttg
     1201 ggcctggaaa tgtacctgca ataatagatg agagtgcaga tataaaaatg gctgtaagtt
     1261 caatcttagt atcaaaatca ttcgataatg gtatgatatg tgcttccgag caagctgtaa
     1321 tagtaccaga aaaaatatat gaagaagtta aaaaagaatt taaatatcgt ggagcacatt
     1381 tcctaaataa agaagaaact gaaaaagtag gaaaagtagt aataattgat ggttcattaa
     1441 atgccagaat agttggacaa ccagcacatg taatagccaa aatggcggat gtagaagttc
     1501 caaaaacagc tagaataata attggtgaag tagaatctgt tgaattaaat gaaccttttg
     1561 cacatgaaaa actttctcct gtacttgcaa tgtacaagtc taaaagtttt gaagatgcag
     1621 ttgctaaagc agaaaaatta gttgcagatg gaggctatgg tcatacatct tcattatatg
     1681 cagactctat aaatcatcca gagagagtag aaaagtttgt aaatgctatg aagacttgta
     1741 gagtattagt taatactcca tcatcacaag gtggtatagg tgatttatat aactttaaat
     1801 tggcaccgtc tcttactctt ggttgtggtt cttggggtgg aaactcagta tctgaaaacg
     1861 ttggagtaaa acatttgctt aatatcaaga cagtagctga aaggagagag aatatgcttt
     1921 ggttcagagc accagaaaag gtttatttca aaaaaggatg tttaggtgta gcagcaagag
     1981 aatttaagga tgttatggat aaaaagaaag catttatagt aacagattct ttcctatata
     2041 ataatggata tactaagaaa ttaactgatt tattagatga gatgggaatt aagcatacta
     2101 cattctttga tgtagctcca gacccaacat tagcttgtgc aagagaagga gctaaagcta
     2161 tggctgattt ccaaccagat ttaataatag ctgtaggtgg aggaagtgca atggatgctg
     2221 gtaagataat gtgggtaatg tatgagcatc cagaagtaga tttccaagac ttagctatga
     2281 gatttatgga tataagaaaa agagtatatg tatttcctaa gatgggtgaa aaagcgtatt
     2341 ttgctgctat cccaacttca gcaggaactg gttcagaagt aactccattt gcagttataa
     2401 ctgaccaaga ttcaggtgta aagtacccat tagcagatta tgaattaatg ccaaacatgg
     2461 ctataattga tgctgatatg atgatggaga tgccaccaag attaacagca gcatcaggtg
     2521 ttgatgcttt aacacatgca ttagaagcat atgtatctat gttaagaact gaaccagctg
     2581 acggtctagc actacaagca ggtaaaataa tatttgaata tttacctcgt gcatataaaa
     2641 atggaaagaa tgataaagaa gcaagagaaa aaatggcaat ggcatctact atggcaggaa
     2701 tgtcttttgc aaatgccttc ttaggaatat gtcactcttt agcacataaa ttaggtgcat
     2761 tccaccatgt tcaacacggt gttgcaaatg cattattaat aaatgaagta atcaagttca
     2821 attgtgcaga agctccaaat aagatgggtg cattctcaca atacagatat cctgattgca
     2881 tacaaagata tgctgaattt gcttcttttg caggtattaa aggaagtaca gaccaagaaa
     2941 aagtagataa tcttataaaa gcaatagatg aactaaaggc aaaagtaggt cttcctaaga
     3001 ctataaaaga ggctggagta gaagaatcta aattcttaga aagattggat gctatggttg
     3061 aacaagcttt tgatgaccaa tgtacaggtg ctaatcctag atacccattg atgagtgaat
     3121 taaaagagat atatctaaaa gtttattatg gtaaataata tctattgtgc aacataggtt
     3181 ctattgggtt tgtatataaa ttattagatt tgaatataaa tagagtttta ttagagacac
     3241 gtagaagtaa ctataaataa tagattataa tttaaattct aaatggaaat gataaaaagt
     3301 cagtaattta atggtaattt cgaatatgtg tatttaatca attaacttta aaattagtcg
     3361 gaaaattttt ttattacata taattttaaa gcataatata taaaaaactt tatgaaaaaa
     3421 ttataaaaat attc
//
LOCUS       DAYAPA010000215         3399 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.220, whole genome shotgun sequence.
ACCESSION   DAYAPA010000215 DAYAPA010000000
VERSION     DAYAPA010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3399
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.220"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(377..559)
                     /locus_tag="ACGI59_003714"
     CDS             complement(377..559)
                     /locus_tag="ACGI59_003714"
                     /inference="COORDINATES: protein motif:HMM:NF015898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HFG9741461.1"
                     /translation="MLNKKLPDTELKIMKCIWHTGYKTVISKDVADEIEKTYGWKHTT
                     TIILLARLTKKGFLIS"
     regulatory      785..882
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            937..1113
                     /locus_tag="ACGI59_003715"
     CDS             937..1113
                     /locus_tag="ACGI59_003715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9741462.1"
                     /translation="MFGNKMDKCTHVLTAYISSSYDYCNFLDTQLDDFILEYGENVVE
                     SCLHQVMVLVSKYN"
     gene            1339..1539
                     /locus_tag="ACGI59_003716"
     CDS             1339..1539
                     /locus_tag="ACGI59_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011723062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFG9741463.1"
                     /translation="MNNGIVKWFNNEKGFGFISMEGRDDVFAHFSAIQTSGFKSLEEG
                     QQVSFDIVKGARGPQAENITIL"
     gene            2544..3389
                     /locus_tag="ACGI59_003717"
     CDS             2544..3389
                     /locus_tag="ACGI59_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFG9741464.1"
                     /translation="MNIIKSITLLLTLLIVILMTGCSPRESASEVAKKYIDKMNSGDY
                     ETAYSLLSKDSKKKISIENLEEFQNILVSTKRIISYKIGKEKIIKKYKHDGKEYKNVV
                     KLEETFNIKNLLYKKDDSLKVNRYFVVENNKYKLLLYESFKKEFGINSMDLAQLKLND
                     FGLKYFKEVDLILKKAIAINPTDSKLYYAQACNYMNFDGFPGYSDTTYNALDSINKSL
                     KYLDKNDSDYKKNASNIYNLKGVILMFHKRFDEAQQYLNKALYFNKDNEDPKYNLNTI
                     KKHLN"
BASE COUNT         1297 a          406 c          418 g         1278 t
ORIGIN      
        1 tatatggcta tttttcaaca ttttacaata tataacattt atttttttaa cttttgtcag
       61 taaaatgtca gcaaaacact ttatccacaa tttttatata agttttccac aacttatcac
      121 caatattatc cacataaaaa aggtagcact tatattatta gctacctttt atttattaat
      181 ctttatattt ccagcttttt atccaacttc ctatttctat tattttttct tcagttattt
      241 cttctccatc atgtaatttt gaaattaatt ctgataaagg attgttatgt aaacctctaa
      301 aaactctttt tccctctatt ttgagatatt ctctctcttt tattaatact gtatagtgta
      361 catgcctccc aattctctat gatattaaaa aacctttttt ggttaatcta gctaaaagta
      421 taattgtagt tgtatgcttc caaccatatg ttttttctat ttcatctgca acatcttttg
      481 atattacagt tttataacca gtatgccata tgcatttcat aatttttaat tctgtatctg
      541 gtaatttttt atttagcata atatattcct tctttcaatt tatgtcactt cttcaaattt
      601 atctagctta aattataata ggagcttttt agaacaaaaa ctatatttaa tttttaccag
      661 gtactctacg tgtttagtct gtttttaagt gtaaaataat gttcatatct ataaatttat
      721 ttaactttca aacaatcctg ttgaataata actaattcta ttgtattatt ttagttgtag
      781 aataaaacta aatcggcaaa actagagaaa tttagtgacg caaagctata gggactaaga
      841 cttatataaa tatcttatga gttatgtcag ccagttgcca aagagatagt gttctttttg
      901 tttttatgaa agttttttag ggggataata atttatatgt ttggaaataa aatggataaa
      961 tgtacacatg tattgactgc ttatattagc agttcgtatg attattgtaa ttttttagat
     1021 acacagttag atgattttat attagagtac ggagaaaatg tagtagagtc ttgtttacac
     1081 caagtaatgg tattggtaag taagtataat tagatagaca ttgatttcta gaaaaccctt
     1141 tttagtttta tctacattta tattaatata tagcaatgta aaacgtttaa atatattttt
     1201 aatattaaga ataattaaaa tgttaactaa aaaatataat taagtagaca tttctattaa
     1261 tctatgatac aatagtattt gtggtaatac acaattaaat caagtatatc ttgatatagg
     1321 ttaataggag tatttttaat gaataatgga atagtgaaat ggtttaataa tgaaaaagga
     1381 ttcggtttta tatcaatgga aggtagagat gatgtgtttg ctcatttctc agctatacaa
     1441 acttcaggat ttaaatcatt agaagaaggt caacaagtaa gctttgatat agttaaaggt
     1501 gctagaggtc ctcaagcaga aaatataact atattataat atatagattt ttcgggaaag
     1561 actcttttta gagtcttttt ttattgttaa tatatgtaag ataatcattg gtgaagtaaa
     1621 taaggtatat tgagtaatgt tcttgatagt ttatatgcta atgaatttca aaaaaataag
     1681 cactcctata aaaagagtgc tcttaatgta aatataaacc ttcattggtc cagcctaata
     1741 ccccgtattc tccaagttct aactattttt tatatacatt atcactttca tccatatcta
     1801 acacaattat ttcttcttta gaatcaattt gtaaaatatg aggactgtgt gttgtgacaa
     1861 ttatttgtgt ttttaggaga attgctttta atgtaataaa aattttacat accacatagt
     1921 tgatataaaa ctataataat tacataagat tgtaataata agacttatct ttacatacca
     1981 catagttgat ataaaacaat gaaattaaca tacaagagac taagacaatt aaaatcttta
     2041 cataccacat agttgatata aaaccccaaa ataaacttag catctccaat gtttacacat
     2101 atacaatgct cttaaaattg cagtgaacgc gcaatagtgc aattgataat acttattata
     2161 tattctcaat gccttatatt tcaactgcta aacctcaatt tgtcacaaat atcgctcact
     2221 gcaaaatttc tatattttta ttatatcata acatataatt aatattttca attaattttt
     2281 acatttaatt atgtttaaat tctataataa tgcaacttta atgtttcttt ctggaactaa
     2341 tataaaaact atttcagaaa gattaggtca tgcagatata aaaataacta tgaatagcta
     2401 ctctcagttt ttagaagaga tggacagaga agcatttgaa aatttgagca aaatactatt
     2461 tattatattt taaagaagat gacttattca atatttgatg ctagaatata attaataaaa
     2521 atttaattat aggtgattgt attatgaata ttattaaaag cataacttta ctcttaacat
     2581 tattaattgt gatattaatg acaggctgtt ccccaagaga aagtgctagt gaagttgcaa
     2641 aaaaatatat tgataagatg aactctggtg actatgaaac agcatatagt ttattaagta
     2701 aagatagtaa aaaaaaaatt agtatagaaa atcttgaaga atttcaaaat attttagtta
     2761 gtacaaaaag aataattagt tataaaatag gtaaagaaaa aatcataaaa aaatataaac
     2821 atgatggaaa agaatataaa aatgttgtta aattagaaga aacttttaat ataaaaaacc
     2881 ttctttataa aaaagatgat tctctaaaag taaatagata ttttgtagtt gaaaataaca
     2941 aatacaagct attattgtat gaaagtttca aaaaagaatt tggtattaat agtatggacc
     3001 ttgctcaact taagcttaat gattttggac taaaatactt taaagaagtt gaccttatac
     3061 taaaaaaagc tattgctatt aatcctactg attcaaagct ttattatgcc caagcatgta
     3121 attatatgaa ttttgatgga tttcctggtt attcagatac aacatataat gctttagatt
     3181 caattaataa atctttaaag tatttagata aaaatgattc tgactacaaa aaaaatgcaa
     3241 gtaatatata taacttaaaa ggagttattc ttatgtttca caaaagattt gatgaagcac
     3301 aacaatattt aaataaagcc ttatacttta acaaagataa tgaagaccca aaatataatc
     3361 taaatacaat aaaaaaacat ctaaactaga tatttaatt
//
LOCUS       DAYAPA010000216         3377 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.221, whole genome shotgun sequence.
ACCESSION   DAYAPA010000216 DAYAPA010000000
VERSION     DAYAPA010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3377
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.221"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            296..451
                     /locus_tag="ACGI59_003718"
     CDS             296..451
                     /locus_tag="ACGI59_003718"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9741465.1"
                     /translation="MDEERKKLIDDAEKLANIGINQILKFRDSLDIENIKYINIVATN
                     LKPNIKK"
     gene            complement(470..826)
                     /locus_tag="ACGI59_003719"
     CDS             complement(470..826)
                     /locus_tag="ACGI59_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFG9741466.1"
                     /translation="MHERIYKIRTSLKMSQREFGEKLGVSRDVISNIEYNRVTPKPVF
                     IQHLCELYNVNKEWLLTGEGEMFIEFSENEKVLKIATDIINEDDKFMKNILFTFSNLN
                     DEQKEFLINLMKNMTQ"
     gene            989..1174
                     /locus_tag="ACGI59_003720"
     CDS             989..1174
                     /locus_tag="ACGI59_003720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9741467.1"
                     /translation="MKTQKTTSHLNNLNLDDDDRKLIQMTLLFKKLNLEQQNYISAMA
                     DGMKVSNELLEKSTMNN"
     gene            1294..2034
                     /locus_tag="ACGI59_003721"
     CDS             1294..2034
                     /locus_tag="ACGI59_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HFG9741468.1"
                     /translation="MSNNLQIFKNNDFGEIRVIELNGEPWFVGKDVAETLGYKDTSDA
                     LKRHVDDEDKGVGEIPTPGGNQNMKIINESGLYSLILSSKLSTAKKFKHWVTRDVLPS
                     IRKTGTYSTKSKDESEIKYMNAQARLKNARAREAKIYLELADKVDIKEYKQIMYSKTT
                     ELLSGETLIPLPKIDKKTYSATEVGKMLGISANKVGGLANAYNLKTDEYGINVWDKAK
                     YSDKQVPNFRYYENVIPVLEKVLKGFEN"
     gene            2091..2684
                     /locus_tag="ACGI59_003722"
     CDS             2091..2684
                     /locus_tag="ACGI59_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9741469.1"
                     /translation="MKENNRIVSVGTESEMDLTAKELNMIDDFKEVMNGVNNEIANDY
                     GFLEIDRDILDKIALSTALNYKKMNFEYSEIDEKLFDDFTFEFMLKFFGDIEEDELIK
                     YLVYIGEESYELNDREKIFYKIVDTLDTIADIKITDQNKIEKEVGMCIGEDNYITYCI
                     DEDLIKFYIKDEEELVINKNSPLLYMLDTLFYEVHEE"
     gene            2753..2971
                     /locus_tag="ACGI59_003723"
     CDS             2753..2971
                     /locus_tag="ACGI59_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9741470.1"
                     /translation="MKILQQFKPYKYDEEKNIEYDEKIIANQNTEVYIVYPRISDEEN
                     RRRWKEFDEVAKEVAINIAMKKAETVES"
     gene            3086..3247
                     /locus_tag="ACGI59_003724"
     CDS             3086..3247
                     /locus_tag="ACGI59_003724"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9741471.1"
                     /translation="MLSLDISKKNVMTLKKDGKFLADIVFKDIKTGKKISVGTLNKKS
                     AGQSNQHNK"
BASE COUNT         1454 a          323 c          572 g         1028 t
ORIGIN      
        1 tggaattttt atacctattc ttgcgaacgt aagttcttga aatattaata tttcttaatt
       61 ttagatgaaa ataggaaata taaaacgttt aaagaagaca agagaataaa gaataaatat
      121 atagggggat gctttaagtg aaaatataaa taatgtacta aatttataca aataaggaaa
      181 taatatatag tgtatttaaa aataaaaagg ctggtggtgt taatttgaag tatttttaag
      241 tgtatattta taatataagg ggaattttta tatttatgaa tggaggaata tagtaatgga
      301 tgaagaaaga aaaaaattaa ttgatgatgc agaaaagcta gcaaacatag gaataaatca
      361 aattttaaag tttagagatt cattggatat agaaaatata aaatacataa atatagttgc
      421 aacaaattta aagccaaaca taaaaaaata gccacccttt tgggtggttt tattgtgtca
      481 tatttttcat taagtttata agaaattctt tttgctcatc atttagatta ctaaaagtaa
      541 ataatatatt tttcataaat ttatcatctt catttataat atctgttgct atttttagta
      601 ccttttcatt ttcagaaaac tctataaaca tttcaccttc gccagttaag agccattctt
      661 tattaacatt atatagctca cataagtgtt gtataaatac aggcttaggt gtaaccctgt
      721 tatattcaat attacttatg acatctctgc ttacaccaag tttttcaccg aattcacgtt
      781 gagacatttt taaggatgtt cttattttat atatacgttc atgcatgatt ttcctcctaa
      841 taattacgtt attaattaga tagtataaca caaaaaagaa ttagtcaaca taaatgttgt
      901 ggtaaaaaca caaaaatatt taaaaacaag tgttgactac acaaaaataa gatgatatac
      961 tgtgtttata acacaaggag gtgctaaaat gaaaacacaa aaaactactt ctcatttaaa
     1021 taatttaaat ttagatgatg atgatagaaa gcttattcaa atgactcttt tgtttaaaaa
     1081 attaaattta gaacaacaaa attatatctc tgcaatggct gatggaatga aagtgtcaaa
     1141 tgagctttta gaaaaatcaa ctatgaataa ctgatacaaa ggtacaaagc tggaataaga
     1201 tttcgaccac aagtgggcga aagatgactg gagtagattt attaggtgtc attcaaaccg
     1261 acaccaaaat aaaaagctag ggaggaagaa attatgagta ataatctaca aatatttaaa
     1321 aataatgatt ttggagaaat aagggttata gaattaaatg gagagccttg gtttgttggt
     1381 aaagatgtag cagagacatt aggatataaa gatacatctg atgctttaaa aagacatgtt
     1441 gatgatgaag ataaaggggt aggtgaaata ccgactcctg ggggtaatca aaatatgaaa
     1501 ataattaatg aaagtggtct ttattcttta atattaagtt ctaagttatc aactgctaaa
     1561 aaatttaaac attgggtaac gagagatgta ctaccaagta taagaaaaac aggtacatac
     1621 agcactaaat ctaaagatga atcagaaata aaatacatga atgctcaagc aagattaaaa
     1681 aatgcaagag caagagaagc aaagatatat ctggaattag ctgacaaagt agatataaaa
     1741 gaatataaac agattatgta ttcaaaaact actgaattac tttctggaga aacattaata
     1801 ccactaccta agatagataa aaaaacttat tcagctacag aggtaggtaa gatgttagga
     1861 atttcagcta ataaagttgg aggattagca aacgcttata atctaaaaac agatgagtat
     1921 ggaattaatg tatgggataa agctaaatat tcagataaac aagtacctaa ttttagatat
     1981 tatgaaaatg taataccagt tttagaaaaa gtactaaagg gttttgaaaa ctaaattcag
     2041 aagttgctaa atataacttt aaaaacttaa atttaaggag gaataagaaa atgaaagaaa
     2101 ataacagaat agtaagtgtt ggaactgaaa gtgagatgga tttaacagct aaagaattga
     2161 atatgataga tgattttaag gaagttatga atggtgttaa caatgaaata gctaatgatt
     2221 atggtttttt agaaatagat agagatattt tagacaaaat tgcattaagt acagctttga
     2281 actataaaaa aatgaatttt gagtattcag aaatagatga aaaactattt gatgatttta
     2341 cttttgagtt tatgttaaag ttctttggtg atatagaaga ggatgaatta atcaaatatc
     2401 ttgtttatat aggtgaggaa agttatgaat tgaatgatag agaaaagata ttttataaaa
     2461 tagtagatac tcttgataca atagcagaca taaaaataac agaccaaaat aagatagaaa
     2521 aagaagtagg tatgtgcatt ggtgaagata attatataac ttattgtata gatgaagact
     2581 taataaaatt ttacattaaa gatgaagaag aactagttat aaataaaaat agccctctgc
     2641 tatatatgtt agacacacta ttttatgaag tacatgaaga gtaaaaattg caataattaa
     2701 ataagtataa gacaagttta tgagctcata ttttataaca aggggtgatg ttatgaaaat
     2761 actacaacaa tttaaaccat acaaatatga tgaagagaaa aacatagaat atgatgaaaa
     2821 aattatagct aatcaaaaca cagaagttta tatagtatat ccacgtatat ctgatgaaga
     2881 gaataggaga agatggaaag agtttgatga agtagccaaa gaagtagcta ttaatatagc
     2941 aatgaagaaa gctgagacag tagaaagtta agaaagtaga gaagttgcta atacttagca
     3001 ctttctcaaa gaagaaaatt ggacaactat ttttaaacaa ctaggaataa ataagcgata
     3061 gtatgtttga aagaaggtga tgaaattgct tagtcttgat attagtaaga aaaatgtgat
     3121 gactcttaaa aaagatggta agtttttagc agatatagta tttaaggaca ttaaaactgg
     3181 taaaaaaata tcagttggaa cattaaataa aaaaagtgct ggtcaaagta accaacacaa
     3241 taaataaaaa aaaacatatg aaaattataa cataaaaagg agagatttaa aatggttgaa
     3301 gttaatataa aaattaaagg cgaagcaagt gaaataattg actcaattag agctatattt
     3361 aatagctttg gtgaaga
//
LOCUS       DAYAPA010000217         3356 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.222, whole genome shotgun sequence.
ACCESSION   DAYAPA010000217 DAYAPA010000000
VERSION     DAYAPA010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3356
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.222"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            198..770
                     /gene="folE"
                     /locus_tag="ACGI59_003725"
     CDS             198..770
                     /gene="folE"
                     /locus_tag="ACGI59_003725"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007287029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HFG9741472.1"
                     /translation="MNKVDKEKIQHAVREILEAIGEDPDREGLIETPNRVARMYEEIF
                     SGLSEEPRDHLKVLFADEKHEELVLVKDIPFYSCCEHHLVPFFGKAHIAYLPKGGRLT
                     GLSKLARVIDTLAKRPQLQERITKNAADIIMEELQPYGVLVVVEAEHMCMTMRGVKKP
                     GSKTVTSAVRGIFEKDIASRAEAMSLITMK"
     gene            834..1625
                     /gene="folP"
                     /locus_tag="ACGI59_003726"
     CDS             834..1625
                     /gene="folP"
                     /locus_tag="ACGI59_003726"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HFG9741473.1"
                     /translation="MFDYGKRTYIMGILNVTPDSFSDGGDFNNLDIAIQHAKDMVDQG
                     ADIIDLGGESTRPGHSYVDSDEELRRVIPVIKKLKQELDIPISIDTYKADVAEEALKL
                     GVTMVNDVWGLRKDKNMASVIGKYDAEVCIMHNQDGTNYDKDIMESIKDFFKVSIEMA
                     MSCGVKKEKIVLDPGVGFGKDFEQNIEVLRRLNELKDLGYPILLGTSRKSVIGKVLPV
                     EPKKRLEGTIATTVLGIRDGVDIVRVHDVYENLMAARMTDAIYRK"
     gene            1636..2001
                     /gene="folB"
                     /locus_tag="ACGI59_003727"
     CDS             1636..2001
                     /gene="folB"
                     /locus_tag="ACGI59_003727"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HFG9741474.1"
                     /translation="MDKILLSNLGFYGYHGVLKEENFLGQKFFVDMELYIDSREAGLS
                     DDINKSVSYAEVYNVVKDITENKQFNLLEALAENIAEEVLNKFILINGVMVRVRKPEA
                     PVNGIYDYFGVEIRRTRDE"
     gene            1994..2500
                     /gene="folK"
                     /locus_tag="ACGI59_003728"
     CDS             1994..2500
                     /gene="folK"
                     /locus_tag="ACGI59_003728"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HFG9741475.1"
                     /translation="MNKAYLGIGTNMGDRFDNLSRACELLKNSDSIYKVKESSLYETK
                     PWGYTEQADFLNMCVEIETEFEPYELLEYCQEIERELHRERIVHWGPRTIDVDVLFFN
                     DVVSTDERLTIPHPRIQDRAFVLIPLMDLNEELIINEKTIKEHLNLLSAEEREEVKEL
                     VGYERKPI"
     gene            2481..3266
                     /gene="aroF"
                     /locus_tag="ACGI59_003729"
     CDS             2481..3266
                     /gene="aroF"
                     /locus_tag="ACGI59_003729"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HFG9741476.1"
                     /translation="MKENLFNNNSKIEINTNNKKITVKKDKLIIAGPCAIESYEQLLE
                     TAKFVKSQGANILRGGAYKPRTSPNSFQGLKKEGLEILKAVKDEVGMAVITELMDVRD
                     MDELYSISDIIQIGSRNMQNFTLLSEVGKQNKPVMLKRGIASTITEWIGASEYIAIEG
                     NSNIIMCERGIRTYNDYTRNTLDLAAVPIIQKETGLPVVVDPSHATGVRYLVKPMSLA
                     SFACGADGIMVEVHPDPENALSDGAQSLCFNEFEDLMKSINNY"
BASE COUNT         1323 a          377 c          683 g          973 t
ORIGIN      
        1 atgggtacca aaagacaaaa aattgctaaa agttttaaaa aaagaaaata atgagctttt
       61 tgaactagtg tcaaaagtaa gtgaaaatta tgactatcaa agacttttag atgtttataa
      121 ttatattttt aaagaaattg agacaaaaga ggttataaaa ctaatcttct agtcacaaat
      181 caatgggggg agtttaaatg aataaagttg ataaagaaaa gatacaacat gcagtaagag
      241 aaatactaga ggcaattggg gaagacccag atagagaagg tctaatcgaa actccaaata
      301 gagttgctag gatgtatgaa gagatattct ctggtcttag tgaagaacca agagaccatt
      361 taaaagttct ttttgcagat gaaaaacatg aagagttagt attggtaaaa gatattccat
      421 tttattcatg ttgtgagcat catctagttc ctttctttgg aaaagctcat attgcttatt
      481 taccaaaagg aggaagactt acagggcttt ctaaacttgc tagagtaata gatacattgg
      541 caaaaagacc acaattacaa gaaagaatca ctaaaaatgc tgcagatata ataatggaag
      601 aattacaacc ttatggtgtc ttagtagtag tagaagctga acatatgtgc atgacaatga
      661 gaggcgtaaa aaaaccaggt tcaaaaactg ttacatcagc agtgagagga atatttgaaa
      721 aggacattgc gtctagagcc gaggctatga gtttgattac tatgaagtag caatttatta
      781 tagatataat catttattga gataaaattt aatttaaggg agtgtcatta attgtgtttg
      841 attatggtaa aagaacttat ataatgggaa tattaaatgt aactccagat agtttttctg
      901 atggaggaga ttttaataac ttggacatag caatacaaca tgcaaaagat atggttgacc
      961 aaggtgcaga tataatagac ctaggaggag agtctacacg cccaggtcat agctatgtag
     1021 actcagacga agagctaaga agagtaatcc ctgtaataaa aaaacttaaa caggaattgg
     1081 atataccaat atcaatagac acttataagg cagatgttgc agaagaagca ttaaaattag
     1141 gtgtcactat ggtaaatgat gtttggggtc ttagaaaaga taaaaatatg gcttctgtaa
     1201 taggaaaata tgatgcagaa gtgtgtataa tgcataatca agatgggaca aactatgaca
     1261 aagatataat ggagtcaata aaagactttt ttaaggtaag tatagaaatg gcaatgagtt
     1321 gtggtgtaaa aaaagaaaaa atagtacttg acccaggtgt aggatttggc aaggattttg
     1381 aacaaaatat tgaagtattg agaagattaa atgaattaaa agatttaggg tatcctattt
     1441 tgcttgggac atccagaaaa tcagtaatag gaaaagtact tccagttgag cctaaaaaaa
     1501 gattagaagg tacaatagct actactgttc ttggtataag agatggcgta gatatagtta
     1561 gagtacatga tgtttatgaa aatttaatgg cagctagaat gactgatgca atatatagaa
     1621 agtaggtata atataatgga taaaatatta cttagtaatc taggattcta tggatatcat
     1681 ggtgttctta aagaagaaaa ttttctaggt cagaaattct ttgtcgacat ggaactttat
     1741 attgattcaa gagaagcagg tcttagtgac gatataaata aatctgtaag ctatgccgag
     1801 gtttacaatg ttgttaaaga tattactgaa aataaacaat ttaatctttt ggaggcttta
     1861 gcggaaaata ttgctgaaga agtcttgaat aaatttatac ttataaatgg agttatggtc
     1921 agagttagaa agcctgaggc tcctgtaaat ggtatttatg actattttgg tgttgagata
     1981 aggagaacaa gagatgaata aggcttattt gggaataggt actaacatgg gtgatagatt
     2041 tgataattta tcaagagcat gtgaactttt gaaaaatagt gattctatat ataaagtaaa
     2101 agaatcaagc ttatacgaaa caaaaccatg gggatataca gaacaagcag attttctaaa
     2161 tatgtgtgta gaaatagaaa cagagtttga accttatgag ttgttagaat attgtcaaga
     2221 aatagagaga gaactacaca gagagagaat agttcattgg ggaccaagaa ctatagatgt
     2281 agacgtacta ttttttaatg atgtagtttc cactgatgaa agactaacaa tacctcatcc
     2341 aaggattcaa gatagagctt tcgttttgat accacttatg gatttaaatg aagaacttat
     2401 aataaatgaa aaaacaataa aagaacattt aaacctccta tctgctgaag aaagagaaga
     2461 agtaaaggag cttgtaggtt atgaaagaaa acctatttaa taataattca aagatagaga
     2521 taaatacaaa taacaaaaag ataactgtaa aaaaagataa acttataata gctggaccat
     2581 gcgcaataga gagttatgag caacttttgg agactgctaa atttgtaaaa agtcaaggag
     2641 caaacatttt aagaggtgga gcatacaagc caagaacatc gccaaattca tttcaaggat
     2701 taaaaaaaga aggtcttgaa atattaaaag cagttaaaga cgaagttggt atggctgtta
     2761 ttacagaact tatggatgta agagatatgg acgaactgta tagtataagt gatataattc
     2821 aaattgggtc aagaaatatg caaaacttta ctcttttaag tgaagtggga aaacaaaata
     2881 aacctgttat gttaaaaaga ggaatagcat ccacaattac tgaatggata ggtgcttctg
     2941 aatatatagc aatagaagga aatagtaata ttataatgtg tgagagaggt ataagaacgt
     3001 ataatgatta tactagaaat acattagatt tagctgcggt acccattatt caaaaggaaa
     3061 caggtcttcc tgttgtggta gacccaagcc atgccactgg agttagatat ttggtaaaac
     3121 ctatgtcact ggcatctttt gcatgtggag cagatggaat aatggtagaa gtacatcctg
     3181 acccagaaaa tgctttgtca gatggtgctc aatctctttg ctttaatgaa tttgaagatt
     3241 taatgaaatc aattaataat tattaaaaga ggcaagagcc tcttttttat tcgtaatcgt
     3301 tgctaaggta aatttataag ttataatttg atagttagat tatttaatat aaataa
//
LOCUS       DAYAPA010000218         3283 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.223, whole genome shotgun sequence.
ACCESSION   DAYAPA010000218 DAYAPA010000000
VERSION     DAYAPA010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3283
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.223"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(290..466)
                     /locus_tag="ACGI59_003730"
     CDS             complement(290..466)
                     /locus_tag="ACGI59_003730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9741477.1"
                     /translation="MFRDKMDRCTHMLTAYIGSSYDYCDFIDTQLDDFILEYGENVVE
                     SCLHQVMVLVSKYN"
     regulatory      complement(520..616)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            1413..1814
                     /locus_tag="ACGI59_003731"
     CDS             1413..1814
                     /locus_tag="ACGI59_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNHCP domain-containing protein"
                     /protein_id="HFG9741478.1"
                     /translation="MNYKNRKRAYYQENSCMDNFICKNCGCLVVPEGAGTQHRNHCPH
                     CLHSLHVDIIPGDRAADCDANMEPIGVWVRKNGEWAIIHRCTRCGHLSSNRIAADDNS
                     VKLMSIALKPLAQPPFPLERIIEKLEKEETE"
     gene            1877..2878
                     /gene="rsgA"
                     /locus_tag="ACGI59_003732"
     CDS             1877..2878
                     /gene="rsgA"
                     /locus_tag="ACGI59_003732"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HFG9741479.1"
                     /translation="MEYHDLYLARIISQEKNFYQAVCVNGVLFAEVSGKLRFEATIQS
                     DFPTVGDFVMLDRCNNKNGNAIIHHVLHRKSLFVRKAAGKVNEEQVVAANIDIVFLCM
                     SLNKDFNIHRLERYLSLTWESGAIPVVVLTKSDLCDNVHRKLQVVNSIALGVDVVVTS
                     SIEPDGYKQISKYIKPGCTVALLGSSGVGKSSLINCLMGGEFLNTKEIRIDDDKGRHA
                     TTRRELFLISGGGIVIDTPGMRELGMWDVSEGLDKSFADIEAYFGKCHFRNCTHTSEP
                     GCAIWEAIAHQELSPKRWESYRRLKTEAVFADNKSEYLAKKEKKFKNISKINKKIRR"
BASE COUNT         1177 a          405 c          539 g         1162 t
ORIGIN      
        1 tttttaatat agcttatttg caaatttata attttgttaa tctaatcctt caaactaaaa
       61 aatcatttat atttacagaa tttcatttta agagatgttt attttcacat agataagaga
      121 atttaagttc tcatatttat tatttgatac aaatttacat ctttattata tgtattttaa
      181 ataaaaaata aaattatgaa tactagaaat ctatcttaat tagctttcta acataaggct
      241 atttaaagta gataaaacta aaaagggttc tctagaaaac caaatctatt taattatact
      301 tacttactaa taccatcact tgatgcaaac aagattctac cacattttct ccgtactcta
      361 gtataaaatc atctagttgt gtatctataa aatcacaata atcatatgaa ctaccaatat
      421 aagcagtcaa catatgtgta catctatcca ttttatctct aaacatatat attattatcc
      481 ccctaaaaaa ctttcataaa aacaaaaaga atatctcttt ttggcaactg gctgacataa
      541 ctcatagttt attttataag cgttagtccc tatagctttg cgtcactaaa tttctctagt
      601 tttgccgatt tagttttatt ctacaattac aataatacaa tagaattgct tattattcaa
      661 tatacttatt tgaaaattaa ataaatttac aaatatgaac gttattttat atcaaaaaaa
      721 atagactatg catgtagaat atatggaaaa acttgattgt aggttttgta tgaaaagaca
      781 gttgttatta atttaagtga gataaattga gcaacaggca taaaatgaga aaacaaataa
      841 tagcagttaa tttttattat taatactagg tgaacattaa atttcaaaat accactagat
      901 tttatagaat aagaaaatga gacaaacata aatatatgaa tatattaaat tatacttgaa
      961 catacttata ttttatgata aaatttcaat aataattatt agtgaaggag tgtcttgcct
     1021 gatgtcagag ttatatataa tggtaaccaa caaataaata gttggagacg catatgggtt
     1081 ctgtttaatc agaattttta agtgtatttt tgatacattt gattgttgtg tcttactctg
     1141 cctttataga taactaacat tagacaacaa ataataatat tacatattac tttgttaaat
     1201 tgtggtgtag tgggctttaa tctgctacac ttttattaat tttacacagt attattttga
     1261 atataattat gttgaaatag aagatactaa attttctagt ccacaggggt tttctgtttt
     1321 tgtgtaggta aatatgtata ccacataaaa aggcagatat aaatacctgt ggactttttt
     1381 tgtagaaaat acacgatacg gaggaaataa aaatgaatta taaaaataga aaacgagctt
     1441 attatcagga aaattcctgt atggataact ttatttgcaa aaattgtggt tgccttgtag
     1501 ttcctgaagg agctggtaca caacatagaa atcattgtcc acattgttta catagtcttc
     1561 atgtagatat cattcctggt gaccgtgctg cagattgtga tgctaatatg gaacctattg
     1621 gagtgtgggt gagaaaaaat ggtgagtggg caattattca tcgttgtaca cgctgtggac
     1681 atcttagttc taatcgtatt gcagctgatg ataattcagt gaaattaatg tcaattgcat
     1741 taaaaccact ggcacagccc cctttcccac tagaaagaat cattgaaaag ttggaaaagg
     1801 aggaaacaga atgaatggta tgaaacaaat caatttacaa gactatggtc taaatagaaa
     1861 ttttattact caaagtatgg aatatcatga tttatacctt gctcggatta tctcgcagga
     1921 aaagaacttt tatcaagctg tatgtgtaaa tggtgtactg tttgcagagg tttcaggaaa
     1981 gctgcgtttt gaggctacta tacaatctga ttttcccaca gttggtgatt tcgttatgtt
     2041 ggatcgttgc aataataaaa atggtaatgc tattattcat catgtgcttc atagaaaaag
     2101 cttgtttgtg cgtaaggctg ctggaaaggt aaatgaggag caggtggttg cagctaatat
     2161 tgacattgtt tttttgtgca tgtcactgaa taaggatttt aacattcata gattggagag
     2221 gtatctgtct ttaacatggg aaagtggagc tatcccagta gtagtactta ctaaatcaga
     2281 cttatgtgac aatgtgcatc gtaaattaca agtagttaat tctatagcct tgggtgtaga
     2341 tgtagttgtt acctcatcta tagagccaga tggttataaa cagatttcta agtatattaa
     2401 accaggctgt actgttgcct tacttggttc ttcaggagtt ggaaaatctt cattaattaa
     2461 ttgtctgatg ggtggagaat ttttaaatac aaaggaaatt cgcatagatg atgataaagg
     2521 aagacatgcc acgactagac gagagctatt tttaatttca ggtggtggta ttgttattga
     2581 cacaccaggt atgagagagt tgggaatgtg ggatgtttca gaaggtttag ataaaagttt
     2641 tgcagatata gaagcttatt tcggaaaatg tcattttcgt aattgtactc acacctcaga
     2701 accaggctgt gctatctggg aagctattgc tcatcaagaa ctttcgccta aaagatggga
     2761 atcctatcgt aggcttaaaa ctgaagcagt atttgcagac aataaaagcg aatacttagc
     2821 aaaaaaagaa aagaaattta aaaatatctc aaaaataaat aagaagatta gaagataagt
     2881 gtagtttttt tgttcattct atgagtctat atactattaa tatattaaat ttgccatcaa
     2941 tttgttactt taatgtgatt ttgatggcaa atttggtttg tggtattatt taaagcaatt
     3001 tattaatttt tttaataaag tttgattttt tatatgatga tatatacatg tgataatgta
     3061 tacatatttg ttacgatagg ctcctttata aagaggtctt atgtaagata agaaaaggca
     3121 tatttatact aatacataat agagtatatg cttatgtatg atagagatgt ttcttaagag
     3181 caaaactgca atattgctaa tttataaagt aaaaatatat gaattataaa atacaaataa
     3241 tatatttata aaaattattg ttttaattta taataaaaat ttt
//
LOCUS       DAYAPA010000219         3236 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.224, whole genome shotgun sequence.
ACCESSION   DAYAPA010000219 DAYAPA010000000
VERSION     DAYAPA010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3236
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.224"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            222..926
                     /locus_tag="ACGI59_003733"
     CDS             222..926
                     /locus_tag="ACGI59_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFG9741480.1"
                     /translation="MSKLIYIADDEDNIRNLVKTFLKNEGHDVMDFKTGDELLEQFNI
                     KECDLVILDIMMPGSSGFEVCTKLREKSTVPIIMLTARDTDIDYITGITLGSDDYFTK
                     PFSPMSLVMRVKSIFRRIEFEKKQNYDKYSNSIDMELKFGDVIINKKNKIVTSKNVNI
                     DLTPNEYNLLTYLFENIDRAVSRDELLNKIWGYDIEVETRAADDTVKRLRKKILNTNI
                     LIETVWGFGFRLKEKS"
     gene            928..2415
                     /locus_tag="ACGI59_003734"
     CDS             928..2415
                     /locus_tag="ACGI59_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFG9741481.1"
                     /translation="MKNGNDKNRIGKIKSKFKLKFSKKNISIKTRILLSILCLIIIVF
                     MIILVSFNVFLDTYIKTTANEELTKSTEIVEHMDSNFRPIKPPQDDGKLPPKVLSNFM
                     RNVQDKVRMAETQSDADAMVVDSKYNLIFPTREDDFLKNVDEMELIRECIQNEKLDLN
                     SDENTRISTRNKDYYISTVKITSIYGEQDKYLILFIDISKTLNLAQKINIVLISVMCF
                     AGILAIFTAVILSEKIAKPIKELCEFAKKMGQGDFKRTYFDFSDKELVELSTVMNKSA
                     EYLDKYDNEQKIFFQNASHELRTPLMSIKGYAEAIKYNVIDSKHASDIILEESDRLSD
                     MVEDLLYISKIDNITKDFELVECDLREVLSNCGARQNVRAINKGIKFIYEFDEEMVLF
                     ECDEKNISKAFMNLIENALRYAKSEIKIVCKYNQKNIVVIIEDDGIGIKKEDLPHVFE
                     RFYKGVGGNHGIGLSIVKSIVNKHGGRIYVENGKKGAKFTILFKL"
     gene            complement(2712..>3236)
                     /locus_tag="ACGI59_003735"
     CDS             complement(2712..>3236)
                     /locus_tag="ACGI59_003735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9741482.1"
                     /translation="IKLIYKIPTLDIKLRNFKLSRLKNLLTSKIETDRNKLNIGIKNK
                     GSQYFMGISKCTSFLNRQIILQVNKKTILDKIVANTLILLFSSIISKPSFTNKTKILV
                     TTISDSIKNILNKTVVNNRVFKFPLYKNDVEKIRMKIKGIVILGFKVIKLQNPKHIIK
                     PSLAYLTTFIYPSF"
BASE COUNT         1280 a          292 c          545 g         1119 t
ORIGIN      
        1 aagtatttct aaaataagtt taataaattc tataataatt tattttttgc cttaaaattg
       61 ccttaaaatc caaacaattt taaaactaat gtactataaa aagctggtat aatttaaata
      121 tagtaaaaaa taagatttat cttatctatt gggatttaat tatttaatca atatatttat
      181 accatttaat ttaaataaaa agtctattaa ggagtgagct tatgagtaag ttaatttata
      241 tagcagatga tgaagataat ataagaaatt tggtaaagac attcttaaaa aatgaaggtc
      301 atgatgttat ggattttaaa acgggtgatg aacttttaga acaatttaat ataaaggagt
      361 gtgacttagt aatactagat ataatgatgc ctggctcaag tggttttgaa gtatgtacaa
      421 aacttcgtga gaaaagtaca gtcccaataa tcatgcttac tgcaagagat acagatatag
      481 attatattac agggataacc ttaggtagtg atgattattt tacaaaacca tttagcccaa
      541 tgtctttggt tatgagagta aaatctatat ttagaaggat agagtttgaa aagaaacaaa
      601 attatgataa atatagtaac tcgatagata tggaattaaa atttggtgat gtaataataa
      661 ataaaaagaa taaaatcgtt acaagtaaaa atgtaaatat agaccttaca ccaaatgaat
      721 ataacctatt gacttatttg tttgaaaata tagatagagc agtttccaga gatgaattat
      781 taaacaaaat atggggatac gatatagaag ttgagacaag ggctgctgat gatacagtga
      841 agagattacg taaaaaaatt ttaaatacaa atattttaat agaaactgta tgggggtttg
      901 gatttaggtt gaaggagaaa agttaagatg aagaatggaa atgacaagaa tagaattgga
      961 aagattaaat ccaaatttaa attaaagttc tcaaagaaaa atataagcat aaaaactagg
     1021 attttgttga gtatactttg tttgataatt atagtattta tgataatatt agtatctttt
     1081 aatgtatttc ttgatacata tataaaaaca actgcgaatg aagaattgac aaagtctaca
     1141 gaaatagtag aacatatgga tagtaatttt agacctatta agccaccaca agatgatgga
     1201 aagcttcctc ccaaagtatt atctaatttt atgagaaatg tacaagataa agttaggatg
     1261 gcagaaacac aatcagatgc tgatgctatg gtagttgatt ctaagtataa cctgatattt
     1321 ccaacaagag aggatgattt cttaaaaaat gttgatgaga tggagctgat tagggagtgt
     1381 attcaaaatg aaaaattaga tttaaattca gatgaaaata cacggatatc taccagaaat
     1441 aaggattatt atatttcaac tgtaaaaatt actagtatat atggtgaaca agacaagtat
     1501 cttatacttt ttatagacat atcaaaaact ctaaatctag cacaaaagat aaatattgta
     1561 ttaatatctg taatgtgttt tgctggaata ttagcaatat ttacagctgt gattttatct
     1621 gagaaaattg ctaaaccgat aaaagagttg tgtgagtttg caaaaaaaat gggtcaagga
     1681 gatttcaaaa gaacctactt tgacttttca gataaagaat tagtagagct ctcaactgtt
     1741 atgaataaga gtgctgagta tctggataag tatgataatg aacaaaaaat attttttcaa
     1801 aatgcatctc atgaattaag gacaccactt atgtctatta aaggttatgc agaagctata
     1861 aaatataatg ttatagatag taaacatgca agtgatataa ttcttgaaga aagtgatagg
     1921 ttaagtgata tggttgaaga tttattatac atttctaaaa tagataatat aactaaagat
     1981 tttgaacttg tagaatgtga tttaagagaa gtactttcaa attgtggagc taggcaaaat
     2041 gtaagagcta taaataaggg gataaaattt atttatgaat ttgatgaaga aatggtttta
     2101 tttgaatgtg atgaaaaaaa catttccaaa gcatttatga atcttataga aaatgctttg
     2161 agatacgcta agagtgaaat aaaaatagtg tgtaagtata atcaaaaaaa tatagttgtg
     2221 ataatagaag atgatggaat tggaatcaag aaagaagatt tacctcatgt ttttgaaaga
     2281 ttctataagg gagtaggggg aaatcatgga attggattat caatagttaa atctattgta
     2341 aataagcatg gtgggcgtat atacgtagaa aatggcaaaa aaggagctaa atttactata
     2401 ttatttaaat tgtaaaaaaa ttagagaaaa tatagagatg tttgaagttg ttttttatat
     2461 atatctgttg tatactgttg aaaaaagaac aatattaagc tgacatatat tacgatagtt
     2521 gacatttaaa gtaaatgctt aaatatagat acgaaaggaa aagtaaaatc tgaagtgtat
     2581 ttaggtttaa gtaagcttct tattttataa aaaatatgta tgaaagaaca gctgattttt
     2641 aatattacaa atttagtaac ggttgtattt gaagcttttg tcatccatat gtttttatca
     2701 gactttttag gtcagaaaga agggtatata aaagttgtaa gatatgctaa actgggtttt
     2761 ataatatgtt taggattctg taatttaata actttaaacc ctaaaatcac aatgccttta
     2821 attttcatcc ttattttttc aacatcattt ttatataaag gaaatttaaa gacacgattg
     2881 tttactactg ttttattaag tatattcttt atactgtcag aaattgtagt tacaagtatt
     2941 tttgttttat ttgtaaaaga aggctttgaa ataatgcttg aaaataatag tataagagta
     3001 ttggcgacaa ttttatctaa aattgtcttt ttgttgactt gtaaaattat ttgtctattt
     3061 aaaaaggatg tacatttaga tatgcccata aagtattggc tccctttatt tttaatacct
     3121 atatttagtt tatttctgtc tgtttctatc tttgatgtaa gtaaattttt tagtcttgaa
     3181 agtttaaaat ttctaagctt aatatctagt gttggtattt tatatattaa ttttat
//
LOCUS       DAYAPA010000220         3223 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.225, whole genome shotgun sequence.
ACCESSION   DAYAPA010000220 DAYAPA010000000
VERSION     DAYAPA010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3223
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.225"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            <1..619
                     /locus_tag="ACGI59_003736"
     CDS             <1..619
                     /locus_tag="ACGI59_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="exosporium glycoprotein BclB-related protein"
                     /protein_id="HFG9741483.1"
                     /translation="LVGPTGATGATGATGATGPTGATGATGATGPTGASAIIPFASGI
                     PLSLTTIAGGLVGTPGFVGFGSSAPGLSIVGGVIDLTNAAGTLTNFAFSMPRAGTITS
                     ISAYFSTTAALSLVGSTVTITATLYQSTAPNNSFTAVPGATVTLAPPLTGILSVGSIS
                     SGIVTGLNIAATAETRFLLVFTATASGLSLVNTVAGYASAGIAIN"
     gene            788..1549
                     /locus_tag="ACGI59_003737"
     CDS             788..1549
                     /locus_tag="ACGI59_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase"
                     /protein_id="HFG9741484.1"
                     /translation="MKNKIEISMNNFVKNYHKSNNITTKWQELLFAYASAEDDMFQKL
                     KSVVGTSHAMPKDFLENGKTIITYFIPFDESVNNSNIGGFECSEIWARAYIETNMLIS
                     NLNDFLCEELEKMGANSYAIPGSYNFDSEKLVSDWSQRHVAFIAGLGTFGLNNMLITE
                     KGCCGRIGSIITSLELEPTKRPENEYCLYKHNNSCKKCVNKCVKEALKEDGFNRNKCY
                     EMCMINEKKYTYIGGCDICGKCIVGVPCSVINPSR"
     gene            1858..2136
                     /locus_tag="ACGI59_003738"
     CDS             1858..2136
                     /locus_tag="ACGI59_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3324 family protein"
                     /protein_id="HFG9741485.1"
                     /translation="MSYKKAIHILPEELLELIQEYVDGECIYIPRKSNNKKEWGSNTP
                     TREELSIRNMQIYEDYQVGYDLQYLSEKYYLSLKSIQRIVLQEKRKDI"
     gene            2429..3004
                     /locus_tag="ACGI59_003739"
     CDS             2429..3004
                     /locus_tag="ACGI59_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1062 domain-containing protein"
                     /protein_id="HFG9741486.1"
                     /translation="MKKITWEVEYISQLPVIRYCKKCGKKTEYICSGLFRVNAQHKYL
                     DIWLIYKCSNCDSTWNSTIYSRVNPKSLNPEILEQFHTNDYNLVKQYAMNMELLHRNG
                     AEVGIPEYKIVGQEININDLTQVHIISKYPCQLKVSTLLREKLGLSRKVFDEMLDCGI
                     IRSNSGVDLKKGKLNYEIMLDIGKEKQKSSN"
BASE COUNT         1239 a          378 c          569 g         1037 t
ORIGIN      
        1 attagtaggt ccaacaggag caaccggagc aaccggagcg accggagcaa caggtccaac
       61 aggagcaaca ggagcaacag gagcaacagg tccaacaggt gctagtgcaa taataccttt
      121 tgcatcaggt ataccactat cacttacaac tatagctgga ggattagtag gtacaccagg
      181 ttttgttggc tttggtagtt cagctccagg attaagtata gttggtggag taatagacct
      241 tacaaacgca gcaggaacat tgactaactt tgcattttca atgccaagag ctggaacaat
      301 aacatctatt tcagcatact tcagtacaac agcagcactt tcacttgttg gctcaacagt
      361 tacaattaca gcaacacttt accaatctac tgcaccaaat aactcattta cagctgtacc
      421 aggagcgaca gttacactag ctccaccact tacaggaata ttatcagttg gttcaatttc
      481 tagtggaatt gtaacaggat taaatatagc agcaacagca gaaactcgat tcttactagt
      541 atttactgca acagcttcag gtctttcatt agttaatact gtagcaggat atgcaagtgc
      601 aggaattgca ataaattaga ttagataaga aaaggcttct aatatcgtat tgatattagg
      661 agctttttta agaatgattg atgaacatag ttttaatatt aattagtgcg catattataa
      721 aatactatta tataatataa aataataaca ttaaattgga aatatagaat tatctagggg
      781 tgatactatg aaaaataaga ttgaaatatc aatgaacaac tttgtaaaaa attatcataa
      841 atcaaataat ataactacaa agtggcaaga acttttgttt gcttatgcat ctgctgaaga
      901 tgatatgttt cagaagctaa aaagtgttgt aggaacatct catgctatgc caaaagattt
      961 tctggaaaat ggaaaaacta taattacata ttttatacct tttgatgaaa gtgtaaataa
     1021 tagtaatatt ggagggtttg aatgttctga aatatgggca agagcgtaca tagaaactaa
     1081 tatgttgatt tcaaatttaa atgatttctt atgtgaagaa ctagagaaga tgggggctaa
     1141 ttcatatgct attcctgggt cgtataattt tgacagtgaa aaattggtaa gtgactggtc
     1201 acaaagacat gtagcattca tagcaggtct tggaactttt ggtttaaata atatgcttat
     1261 tacagaaaaa gggtgttgtg gaagaatagg aagcataatc acaagtttag aattagagcc
     1321 aactaaaaga ccagaaaatg aatactgctt gtataaacat aataatagtt gcaaaaaatg
     1381 tgtcaacaaa tgcgtgaaag aagctttaaa agaagatgga tttaatagga ataagtgtta
     1441 tgaaatgtgt atgataaatg aaaaaaaata cacttatata ggtggatgtg atatttgtgg
     1501 aaagtgtata gttggagttc cttgttcagt aataaaccca tcaagataat ggatttaagt
     1561 tgactaataa ttttaaagat agtttataat gaaaaaattt tagttatcaa aataaatata
     1621 atataaaaac taaataaatg tgtttaaaaa atggataaat aatttgtgtt gattgacaac
     1681 cagttatact gttgatagac ttatatgtat cgctgtgata tataaggagg gttgagattt
     1741 tatgtttgat ttaatcttta tttttgcata gcaagggcta ttgcaactat gaaatttaat
     1801 atataaatag ccctttgcta ttcctaatga aaaattacta tttaatagga gagcaaaatg
     1861 agctataaaa aagcaataca tatattacca gaagaattac ttgaattaat acaggaatat
     1921 gtagatggtg aatgtatcta tattcctaga aaatctaata ataaaaaaga atggggaagt
     1981 aatacaccta ctcgtgagga gttgtcaatt agaaatatgc agatttacga agactatcaa
     2041 gttggatatg atttgcaata tttatcggaa aagtattatt tatctttgaa aagtatccaa
     2101 agaattgtat tacaagaaaa aagaaaagat atctaaggat gagccagtat agttttataa
     2161 ttatactggc tcattttctt ttgataaaag tgtttgaggt atagtttctt ttaagtggat
     2221 gatataattt aagtaatttc aataacttat acagtagcta acaaagctgg ttgtaaagta
     2281 tctaataaga agtagtagct ttttatatgt ttgaaattaa aatttattaa aattaggagg
     2341 tcttgttttg aaaaaaatac ttgattatat tccacctttt cataactttc tattaattta
     2401 gtaatcttat agaaaggaaa ttgcatttat gaaaaaaatt acttgggagg tggaatatat
     2461 ctcccagtta cctgtaatta ggtattgtaa aaaatgtgga aaaaagactg aatatatttg
     2521 ttcaggttta tttagggtta atgctcaaca taaatatttg gatatatggt taatatacaa
     2581 gtgttcaaat tgtgattcaa catggaactc aactatatat tctcgtgtta atccaaaaag
     2641 cttaaatcca gaaatactag aacaattcca tacaaacgat tataatcttg ttaagcaata
     2701 tgctatgaat atggaacttt tacatagaaa tggagcagaa gttggtattc cagagtataa
     2761 aatagtagga caagaaatta atattaatga ccttacacaa gttcatatta ttagtaaata
     2821 tccatgtcag ttaaaggtat caacattatt acgtgagaaa ctaggtttat ctaggaaggt
     2881 atttgacgaa atgttggatt gtgggattat tagaagtaac tcaggagttg atttaaagaa
     2941 aggtaaatta aattatgaaa ttatgttaga tataggcaag gagaagcaaa agtcttcaaa
     3001 ttgattagaa taactaactt aatatatttt caggaacatc atttaaaaaa atagtggctt
     3061 taaaaaattt aaagccacta ttttttaata tgttttatat taatatttaa aatatagtgt
     3121 gtattcttca aaattatagc gataattaaa atattactat aaaaaggaag gtgcatactt
     3181 tttatttaac aaaaactaga tattaaaaac ttttcgagga ata
//
LOCUS       DAYAPA010000221         3097 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.226, whole genome shotgun sequence.
ACCESSION   DAYAPA010000221 DAYAPA010000000
VERSION     DAYAPA010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3097
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.226"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            <1..1107
                     /locus_tag="ACGI59_003740"
     CDS             <1..1107
                     /locus_tag="ACGI59_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFG9741487.1"
                     /translation="ISIIISDESMDVIDLYDDNNNEVAKKVNFSIFNPKFNIYMYQYK
                     KLINYLDKSICMGALSSGSTSVPKVLYRTYESWAGFFPIQNGIFNISGESILFVNGTL
                     SFTGNLNSIMSVLYEGGSIVISSGLNCKSWIRTIKQYNVTNIYLIPTKLQLLVKHLKE
                     PVLKVVSIFTGSQLLFEDTARNLKKYMPKSEIILYYGASELNYITYLCYDELIEKPLS
                     VGRPFPGIDIYIREGKIFVNTEYAVYNATKPYSVNDIGYYDNDGYLIFEGRSDDIINI
                     GGFKVSSTKIENEVKKIPQIEDAIVLPYSDTIRGNQIVLFVITIDKITKKDLLIKMKD
                     NLMKNEIPKKIIFLNSFPYTSSEKIDRLALLKML"
     gene            1183..2058
                     /gene="dpsA"
                     /locus_tag="ACGI59_003741"
     CDS             1183..2058
                     /gene="dpsA"
                     /locus_tag="ACGI59_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit DpsA"
                     /protein_id="HFG9741488.1"
                     /translation="MSNQYDITVIGGDLRQVYMVKKLINKGYSVVTYGISNEIINGIV
                     GKSTSLAEALSKSSIILCPIPFTKNQVNIENTDDCLDATIDNLLINLSPNQILFGGNI
                     PTKVCKFCEQHSIQIYDFMKMNDVAILNAVATAEGAIAEAIKRSIINIHQSNCLVLGY
                     GRCAQILAKKLYALDANVCVGARNNDARSTANAFGYSSISLSQLDEKLLQFDYIFNTI
                     PECILTKERLEKVSQEVTIIDIASSPGGVDYSVVKEAGINATLCLGLPGKYSPKTSGE
                     ILVNAIENIINERRD"
     gene            2061..2651
                     /locus_tag="ACGI59_003742"
     CDS             2061..2651
                     /locus_tag="ACGI59_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit B"
                     /protein_id="HFG9741489.1"
                     /translation="MRLEGLTIGVGFTGSFCTYDKIFIELENLVKEGANVHTIFSDVS
                     QNIDCRFGNSEEFMKKAYELTGNKPIVTIEEAEPFGPKGIADIIIIAPCTGNTAAKLA
                     NGITDSPVLMAAKGHLRNDKPLVISISTNDALSFNFKNIGILLNSKNIYFVPFGQDNC
                     KAKPNSMIAHTELIIPTIELALENKQLQPVIKSPHQ"
     gene            complement(2858..3058)
                     /locus_tag="ACGI59_003743"
     CDS             complement(2858..3058)
                     /locus_tag="ACGI59_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9741490.1"
                     /translation="MEKLKTISVFSLIISVILTIGGIGIVTYYVDNLFIRGLSVFVLI
                     MSSSFVSTTVRLIFEESKRYKF"
BASE COUNT         1182 a          392 c          481 g         1042 t
ORIGIN      
        1 atttcaatca ttatatctga tgaatctatg gacgttattg atttatatga tgataacaat
       61 aatgaagtag caaaaaaagt taatttttct atttttaatc ctaaatttaa tatatatatg
      121 tatcaatata agaagcttat aaattattta gataaaagta tttgtatggg agctttatct
      181 tcaggttcta caagtgttcc aaaggtatta tatagaacat atgagagttg ggctggattt
      241 tttcctatac aaaatggtat ttttaatatc tctggggaat caatcttgtt tgttaatgga
      301 acacttagtt ttacagggaa tctaaattct ataatgtcag tattatatga aggaggtagt
      361 attgtaataa gttcaggtct taattgtaaa tcatggataa ggacaataaa acaatataat
      421 gtaactaaca tataccttat acctacaaaa cttcaacttt tggttaaaca cttaaaagaa
      481 ccagtactca aagttgtaag tatttttaca gggtctcaat tattatttga agatactgca
      541 agaaatctaa aaaaatatat gccaaagtct gaaattattt tatactatgg ggcaagtgaa
      601 ttaaattata ttacatattt atgctatgat gaactaattg aaaaacctct aagtgtaggc
      661 agaccatttc caggaataga tatatatatt agagaaggaa aaatttttgt gaatacagaa
      721 tatgcagtat ataatgcaac caaaccttat tctgtcaatg atattggata ttatgataat
      781 gatggatact taatatttga aggaagaagt gatgacatta taaatattgg aggatttaaa
      841 gttagttcta ctaagattga aaatgaagtt aaaaaaattc ctcaaattga agatgcaatt
      901 gtattgccgt actcagacac tataagagga aatcaaattg tattatttgt aataactata
      961 gacaaaatta cgaagaaaga tttgttgatt aaaatgaaag ataatctaat gaaaaatgaa
     1021 atcccaaaga aaattatctt tttaaattca tttccatata cctcttctga aaagatagat
     1081 agattagcat tattaaaaat gttataagta taatatttag taattttagt gaaccaatgt
     1141 attgataaaa acatattgta tatagtaaag gaggcttaca atatgtcaaa tcaatacgat
     1201 ataactgtta ttgggggaga cctgcgacaa gtgtacatgg taaaaaaact aattaataaa
     1261 gggtattctg tagtcactta tgggatatca aatgaaatta taaatggaat cgttggaaaa
     1321 tcaacttcac tagctgaagc actatccaaa agttccatta ttttatgtcc aattccattt
     1381 acaaagaatc aagttaatat tgaaaatacg gatgattgtc ttgatgctac tattgataac
     1441 ttgctaatta atttatctcc taatcaaata ttatttggag gtaacatacc aactaaagtt
     1501 tgtaaatttt gcgagcaaca ctctattcag atttatgatt ttatgaaaat gaatgatgtt
     1561 gctattttaa atgcagttgc tacagctgaa ggggctatag cagaagctat taaaagaagt
     1621 ataatcaata tacatcaaag taactgttta gtattaggtt atggaagatg tgcacaaata
     1681 cttgcaaaaa aattatacgc tttggatgca aatgtatgtg ttggcgcaag aaataatgat
     1741 gcaagaagta cggctaatgc atttggatat tccagtattt ctttaagtca acttgatgaa
     1801 aaacttttac aatttgatta tatttttaac accataccag agtgtatttt aacgaaagaa
     1861 aggttagaaa aagtatcaca agaagtaact attatagata tagcttcttc tcctggagga
     1921 gtagattatt ctgttgttaa agaagcaggg attaatgcga ctctttgcct tgggcttcct
     1981 ggtaaatatt caccaaagac atctggtgaa attcttgtta acgctataga aaatattata
     2041 aatgaaagaa gggattagaa atgagacttg aaggacttac aattggagta ggatttactg
     2101 gttcgttttg tacttatgat aaaatattta tagagttaga gaatttggtt aaagaaggag
     2161 ctaatgtaca cactatattt tcagatgtat cacaaaatat cgattgtaga tttggaaatt
     2221 ctgaagagtt tatgaaaaaa gcatatgaac ttacaggaaa taaacctata gttacgattg
     2281 aagaggcaga gccatttgga cctaaaggaa ttgctgatat tataataata gctccttgta
     2341 ctggaaatac tgctgcaaaa ttagcaaatg gtattacaga ttcaccagta ttaatggcag
     2401 caaaaggtca tcttcgtaat gataaaccac tagtaatatc aatatcaacg aatgatgctc
     2461 taagctttaa tttcaaaaat attggcattc tccttaattc taaaaatata tattttgtac
     2521 cttttggtca agataattgc aaagcaaagc ctaattctat gattgcacat actgagctaa
     2581 taattccaac tatagaactt gctctagaga acaaacagct acagcctgtt ataaaaagtc
     2641 cacatcaata gaattggttc ttataatatt atgtattaga tacaatacta gcaaagaggc
     2701 tgtctcaaaa tagagagaag cctctttttc ttgttgaaat gatgaaaata tatttaattt
     2761 catacaaaaa aatgcctcat ggactaaaaa gtctattttg agctaatctc cgtaattttt
     2821 gctatattaa aattatttac ttaatccaat ttttaaatta gaatttatat cgttttgact
     2881 cttcaaatat caatctaact gttgttgata caaaagaaga ggacataatt aaaacaaata
     2941 ctgatagtcc tctaataaac aaattatcta cataataagt tactattcca atacctccaa
     3001 tcgttaaaat tacacttata atcaaagaaa aaacgctaat tgtttttaat ttttccatgc
     3061 ttatcctcct tcttataaac ttcaaattta tctatat
//
LOCUS       DAYAPA010000222         3013 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.227, whole genome shotgun sequence.
ACCESSION   DAYAPA010000222 DAYAPA010000000
VERSION     DAYAPA010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3013
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.227"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            157..525
                     /locus_tag="ACGI59_003744"
     CDS             157..525
                     /locus_tag="ACGI59_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFG9741491.1"
                     /translation="MENLVKIFKALSDETRLNILILVSKRNICQKGISKYLGISDSAV
                     SQHIKILKDVGIITGYKEGYYVLYHINKESFNICVDFINSMLSNSSESFIDVFDVNTI
                     HLGCSKECKSIKKCCKRREK"
     gene            528..899
                     /locus_tag="ACGI59_003745"
     CDS             528..899
                     /locus_tag="ACGI59_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HFG9741492.1"
                     /translation="MKVCIPVEENKGLDSKPYGHFGSAPIFVVCDLESGEVKSLDNGD
                     LDHEHGKCQPLKALSGTAVDAVVVGGIGQGAIIKLNGMGIKVYRAEGDTISANLDLLK
                     NGKLVEFPSDHTCSHDGCGHH"
     gene            complement(1044..1499)
                     /locus_tag="ACGI59_003746"
     CDS             complement(1044..1499)
                     /locus_tag="ACGI59_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9741493.1"
                     /translation="MNINTVQGDSIEVLLRQLGATRISKVSSTLYFIKFDLGDGWEIS
                     YTYNINAKDQYFLQRIEPYPIGRGLFNDEYEIVSFISKDLKKFLNAKNSSNFKTFVEV
                     TRKVNSIIDNVEELFLNYNVDGDDLKTLNKELNDILNDIDYIEKHTKKI"
     gene            complement(1538..2803)
                     /locus_tag="ACGI59_003747"
     CDS             complement(1538..2803)
                     /locus_tag="ACGI59_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HFG9741494.1"
                     /translation="MEFQQIIAICIFLIVMAAIITEKVNRSVAAVGGALLMIIFNILT
                     LDEGLSHIDFNTIGVLVGMMLFVAVVKNSGLFEYIAIWTAKKAKGDPWKIMICFAIIT
                     AILSAVLDNVTTVLLIGPMTIVITQILGLNPVPFLITQILASNIGGTATLIGDPPNIM
                     IGSAANLSFMDFVINLGPAVIVILAITIICFRFIYGKELVVNERAKNAILKLDEKKSV
                     KDKPLLIKSLILIAFILFGFMFHSTIHIDSSVVALTGASIMLLIGKQDVDEIMAGIEW
                     STILFFMGLFVVVGGLVEVGIINKLAQALIGLTEGHLVFTMLLILWLSAIVSSFLDNI
                     PFVATLIPLILTMQAEGIDVMPLWWATSLGACLGGNGTLIGASANVVLAGIGNKHGHP
                     ISFKEYFKIGFPLMIISIIISTVYLIIKF"
BASE COUNT         1124 a          413 c          411 g         1065 t
ORIGIN      
        1 tatatgtaat tacttttatt aataaaactc aaagttatat tagtaaaaat tggaattatt
       61 tataatatac tccaaaattt aaaaataatg gttgaaaaat ctaattaaga gtactataat
      121 ttaattaagt aattacttaa tcaaaatgag gtacttatgg aaaacttggt taaaatattt
      181 aaagcattga gtgatgaaac tagacttaac atacttatat tagtatccaa gagaaatata
      241 tgtcaaaagg ggatttctaa atatctaggg atttcagatt ctgctgtttc acaacatata
      301 aaaatattaa aagatgttgg gataataaca ggatataaag aaggatacta tgttttatat
      361 catattaata aagaatcatt taatatatgt gtagatttta taaattctat gttaagtaat
      421 tctagtgaaa gctttataga tgtatttgat gtaaatacaa tacatctagg atgcagtaaa
      481 gaatgtaaat ctataaaaaa atgttgtaag agaagggaga aataataatg aaagtatgta
      541 taccagtaga ggagaataaa ggattggata gtaaaccata tggacatttt ggttctgctc
      601 caatttttgt tgtgtgtgat ttagaaagtg gagaagttaa atctttagat aatggagatt
      661 tagatcatga acatggaaaa tgtcaaccat taaaagcatt gtcagggaca gctgtagatg
      721 cagtagttgt tggaggaata ggacaaggag ctataattaa attaaatgga atgggaataa
      781 aagtttacag agcagaaggt gatactataa gtgctaattt agacttactt aaaaatggaa
      841 aattagtaga atttccatca gaccatactt gctcacatga cggatgtgga catcattaaa
      901 aaaaaacagc tgatgaaata tatacttcat cagctatttt ttcttttaat aaatacgatt
      961 ttattctttt aaaaccaaaa tttagatttt tatgtttttt gtagtatcct taataatatt
     1021 tcaagtttac aagtttacta cttttagatt ttttttgtat gtttttctat atagtcaata
     1081 tcatttaata tgtcattgag ttctttatta agtgttttta aatcatcacc atctacattg
     1141 tagtttaaaa atagttcctc tacattatca atgatagaat tgacttttct agtaacttca
     1201 acaaatgtct taaagttact actattttta gcatttagaa atttttttaa atcctttgat
     1261 ataaatgata ctatttcata ttcatcatta aatagacctc taccaatagg atatggttca
     1321 attctctgta aaaaatattg gtctttagca ttaatattat aagtataaga tatttcccaa
     1381 ccgtctccta aatcaaactt gataaaatat aatgtactac ttacttttga tattcttgtg
     1441 gcaccaagct gtcttaaaag aacttcaatc gaatcacctt gaacagtatt aatattcata
     1501 tatacacatc ccctttagta ttattatact atatttttta aaattttata attaaataaa
     1561 cagtagatat aataatagat ataatcataa gtggaaaacc tattttgaaa tactccttaa
     1621 aagagatagg atgaccgtgt ttattaccta tacctgctag tacaacatta gcagatgctc
     1681 ctattagtgt tccatttcct ccaagacaag ctccaagaga tgttgcccac catagtggca
     1741 ttacatcaat accttcagct tgcatagtca aaataagagg aattagtgtt gcaacaaatg
     1801 gaatattatc taagaaagaa gagactatag ctgacaacca taatatcaac aacattgtaa
     1861 atacaagatg tccttctgta agtccaatca atgcttgagc caatttgttt attatcccaa
     1921 cttctactaa tccaccaact actacaaata atcccataaa gaataaaatt gtagaccatt
     1981 caatacctgc cataatttca tctacatctt gttttcctat taaaagcatt atagatgctc
     2041 ctgtcagtgc tacaacagaa gaatctatgt ggattgtact gtgaaacata aaaccaaaaa
     2101 gtatgaatgc tattaatata aggcttttta taagtagagg tttatcttta acagattttt
     2161 tttcatctag ttttaatatt gcatttttag ctctttcatt cacaacaagt tcttttccat
     2221 aaataaatct aaagcatatt atagttatag caagtattac tataacagca ggacctaaat
     2281 ttattacaaa atccataaaa ctaagattgg cagcactacc aatcataata tttggagggt
     2341 ctcctataag agtagctgtt cctcctatat ttgaagctaa aatctgtgtt attaaaaatg
     2401 gaactggatt aagtcctaat atttgtgtaa taactatagt cattggtcca ataagtaaga
     2461 cggttgtaac attatctaat acagctgata aaatagctgt tataatagca aaacaaatca
     2521 ttatcttcca tgggtcacct ttggctttct ttgcagtcca gatagctata tattcaaata
     2581 accccgagtt tttgacaaca gctacaaata gcatcatacc aactaataca ccaatagtat
     2641 tgaaatctat atggcttaat ccttcatcaa gtgtaagtat attgaatatt atcattaata
     2701 gtgcaccacc aacagctgct acggatctgt ttaccttttc tgtaataata gctgccataa
     2761 ctattaaaaa tatacatata gctattattt gttgaaattc cataatataa gtacctccat
     2821 ttgtattatt ttgtgcctaa tgctagtact atatatagta ttactttttt attgtttttt
     2881 caatggatgt atgaaagtat attatttaac aaagtttttg gaaaattgac agaatttaaa
     2941 gaaaattttt gttatttaaa taaattttaa aattattcca ttgataataa tattaaacga
     3001 caaacgaaaa aat
//
LOCUS       DAYAPA010000223         2994 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.228, whole genome shotgun sequence.
ACCESSION   DAYAPA010000223 DAYAPA010000000
VERSION     DAYAPA010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2994
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.228"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(451..1770)
                     /locus_tag="ACGI59_003748"
     CDS             complement(451..1770)
                     /locus_tag="ACGI59_003748"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFG9741495.1"
                     /translation="MLNINVFWIFLPIFSTAIEWIVLKFLLNEFSELRKNRLTLNSSI
                     VIAIMIITMLNTIEFNINIKLFICIFITYVLYKINYVVDKWKSVFISLLYWMLLIGFD
                     SIGLSIVMTLNSIKNMNDLLEDNLLKLELIMISKSLLIVLIPLLKFIRLKTKINKRDC
                     IYLSIPLIANIIGVIVIFEFIFKDKIIDSKESLIILTISTVFLLSNLSLVSIIGRIIK
                     DNSLKIEYEITKKRMNMQYKYYLNLQEYQLKIRKLYHDMNNHIICIQNVYGKNEFADK
                     YIKDINKQIKECNTIFNTQNMILDVILSDKKSICDMNNIYFLVDINFLECNFIEMPDI
                     CSIFSNIIDNAIEACIKIKDTSIQKKIKLRGTVINRLFVIKCENTKINEVVLKNNKVI
                     TSKKDSFLHGIGISSVKSSVEKYNGNVEIYSDKNKFTITIYIPLTRN"
     gene            complement(1764..1907)
                     /locus_tag="ACGI59_003749"
                     /pseudo
     CDS             complement(1764..1907)
                     /locus_tag="ACGI59_003749"
                     /inference="COORDINATES: protein motif:HMM:NF016296.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
     gene            complement(2031..2741)
                     /locus_tag="ACGI59_003750"
     CDS             complement(2031..2741)
                     /locus_tag="ACGI59_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFG9741496.1"
                     /translation="MYRIVICEDDITQIAFLRECILKSLEGISSQIELFEFNSGEELL
                     ETNLEGIDIFFLDIKMLQLTGMDVAKIIRETNDTSEIIFITSIVDYIQEGYKVRAYRY
                     LLKPIDFGDLNESILSCISDIIKKRENFMLIENKGIINKILINSIMYIEVRKKVLTIH
                     TKNDTYYTKNSMDKIELELEKYNFFRCHKSYLINLEYIQFICKKTVVINDEDVPVSKY
                     RMADLKKKLTYVLGDVLC"
BASE COUNT         1098 a          449 c          197 g         1250 t
ORIGIN      
        1 aaattagtaa aatatttttc tttaatattt attactaata tcaactttac tatctaatta
       61 gttatttaat tgttgttttt atgttcctag ttataaagtt tatcaaattt acattttatt
      121 tttttatact atatagaact tttggtcaat aatgttacca tactaaggtc gcatttgcag
      181 ataaaaataa ctctattttt ctaaatttat ttaagatata tcatgcaaat aatataacta
      241 taaaacctat caacatttta aattcaaaca aatataaatc acattttttg ttctattatt
      301 ttattgagta ctaaaaagga tgttatctta ttagcatagc attcaaccat ttttatcacc
      361 tccaaatatc ctatgcctta aagatagcat cttctatttt atctttcaat actactgctc
      421 caactgggca atttagagcc ccaactagaa ttagttccta gttaatggta tataaatagt
      481 aattgtaaac ttgtttttat ctgagtatat ttccacattt ccattatatt tttctactga
      541 actttttaca ctacttattc ctattccatg taaaaaggaa tctttcttac ttgtaataac
      601 cttattattt tttaatacaa cttcatttat ctttgtattt tcacatttta ttacaaataa
      661 cctatttata acagttcctc ttaatttaat ctttttttgt atacttgtgt ccttaatctt
      721 aatacaagct tctattgcat tatctattat atttgaaaat atactacata tatctggcat
      781 ctctataaaa ttacattcta aaaaattaat atctactaga aaatatatat tattcatatc
      841 acaaatagat tttttatcac ttaaaataac atctagaatc atattctgag tattaaatat
      901 agtattacat tcttttattt gcttatttat atctttaata tacttatctg caaactcatt
      961 tttcccataa acattctgaa tacaaatgat atgattattc atatcatgat atagttttct
     1021 tattttcaac tgatattcct gtaaatttaa ataatattta tattgcatat tcattctttt
     1081 ttttgtaatt tcatattcta tctttaaact attatctttt attattcttc ctattatgct
     1141 tactaatgaa agatttgata gtaaaaatac agttgatatt gtaagtatta ttaaactctc
     1201 tttagaatcg ataatcttat ctttaaaaat aaattcaaat attactatta caccaataat
     1261 gtttgctatt aatggaatag ataaatatat acaatccctc ttgtttattt ttgtttttaa
     1321 tctgataaac ttaagcaatg gtattaatac tattagtaat gatttagata tcattattaa
     1381 ctctaacttc aacaaattat cctctaataa atcattcata ttttttatag aattaagtgt
     1441 cattactata cttaatccaa tactatcaaa tccaattaat aacatccaat atagcaaact
     1501 tataaataca cttttccatt tatccactac ataatttatt ttatacaata catatgtaat
     1561 aaatatacat ataaataatt taatatttat attaaactca attgtattca acatagtaat
     1621 tatcataata gcaattacta tgctactatt tagggttaat ctattctttc ttaactcact
     1681 aaattcattt aataaaaatt ttaatacaat ccattctatc gccgtagaaa atataggtaa
     1741 aaaaatccaa aatacattta tatttaacat aatacatctc ccaatacata tgttaatttt
     1801 ttctttaaat tagccattct atatttacta actggtatat ctttaccatt aataactaca
     1861 gtatttttac atataaattg aatatactcc aaattaatta aatagctctt gtaacatttt
     1921 taaaaattta ttctttctta aatcactgaa ttcatttgat aaaaatttta atacaattca
     1981 ttctatcgcc gtagaaaata taggtaaaaa aatccaaaat acatttatat ctaacataat
     2041 acatctccca atacatatgt taattttttc tttaaatcag ccattctata tttactaact
     2101 ggtacatctt catcattaat aaccacagtc tttttacata taaattgaat atactccaaa
     2161 ttaattaaat agctcttatg acatctaaaa aaattatatt tttctagctc taattctatt
     2221 ttatccatac tatttttagt ataataagta tcatttttag tatgtatagt caaaaccttt
     2281 ttcctaacct ctatatacat aatactatta attaaaattt tattgattat acctttattt
     2341 tctatcagca taaaattttc tctttttttt ataatatccg atatacaact caaaatactt
     2401 tcatttaaat ctccaaaatc tataggcttt aataaatatc tataagccct taccttatag
     2461 ccttcctgta tgtaatcaac tattgaagtt ataaatatta tttcagaagt atcattagtt
     2521 tctctaatta ttttagccac atccatgcct gttagttgaa gcattttaat atctaaaaaa
     2581 aatatatcaa taccttctaa atttgtttcc aataattctt ctcctgaatt aaactcaaat
     2641 agttcaattt gactagaaat accctctaag gactttaata tacactctct taaaaaagct
     2701 atttgtgtaa tatcatcctc acagataact attctataca taaacacccc tccttagaca
     2761 ttagatctga aaacaaataa aatatcaatt tataaataat tatttagtta tacaactaac
     2821 atggtcatca aatactatta ttcacttaaa aaatcacccc ctatttttag tatgttaaca
     2881 acaaaataag aagatatctt agatttttaa aaacaattaa tacttaatgt agttttgtat
     2941 actatttata ggtaattttt tatatcattt taaacattaa aattattgat gttt
//
LOCUS       DAYAPA010000224         2987 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.229, whole genome shotgun sequence.
ACCESSION   DAYAPA010000224 DAYAPA010000000
VERSION     DAYAPA010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2987
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.229"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            52..1179
                     /locus_tag="ACGI59_003751"
     CDS             52..1179
                     /locus_tag="ACGI59_003751"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFG9741497.1"
                     /translation="MKGKITMDFKSLGISENTINILKKSGITTPTPIQKESIKLIKEG
                     KDVIAEAQTGTGKTLAFLLPIFENISLDINDIQVLILSPTRELAIQITEEAMKLKESK
                     DVSILAAYGGKDIGSQIKKLKGNIHMIIATPGRLLDHLNRKTIDLSKLKTFVLDEADQ
                     MLLMGFKNEVEAILKETSNKKQTLCFSATINSQVKKLAYRYTKNPVVVSIQKEEITLN
                     NIKQEVVETTDRKKLDALCKVLDEDNPFMAIIFCRTKRRVDNLEEALAIRGYNCQKLH
                     SDIAQSKRERIMKSFRNLDIQYLIATDVASRGLDISGVSHIYNYDLPETPEDYIHRIG
                     RTGRAGEEGYTCAFIDPKNERMLSEIETAIESKISRRIIEL"
     gene            complement(1556..2740)
                     /locus_tag="ACGI59_003752"
     CDS             complement(1556..2740)
                     /locus_tag="ACGI59_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9741498.1"
                     /translation="MQKSVKKAKVTGSMLAIFGVASVLFSSHAGGGFATGNQETQYYV
                     QYGWTAPLMAILAMIILTATMREVIIMYNNNNCRNYKDLFCELWRPYPKLEIIWEIYY
                     YLMVLIAVSAVIAGAAAVFQSIGVNYFVAVFIIGVVLLVFTIFGAMLVSKAATAMTIA
                     ILVCTLTIFIVGIKAKVPEITEILSNRTSFTPGYIKPILNTFIYAGFQSVVIPTLAGC
                     SRPLLKNSKEATKAMIISFVMNAIALGLAVTMLMGWYREIIAAGQTTLPTLYVAGQSG
                     NHTIYIIYNVALFLCLMSTGVTTIFGLVNRFEDHKALSFLSSRMKRRVFTACAIMVVS
                     MLISLTGLSNIVKYGYGYCGYLGLFTIVIPFLTLGHYKNKKFAKENPEAKWPAELNEN
                     VN"
BASE COUNT         1131 a          447 c          405 g         1004 t
ORIGIN      
        1 atacttacaa atatgatatt attaattggt taataaaaaa gaaaattttt catgaaaggt
       61 aagataacaa tggatttcaa aagcttaggg attagtgaaa atacaataaa tattctaaaa
      121 aaatcaggaa taacaacacc tacacctata caaaaagaaa gtattaaact aataaaagaa
      181 ggaaaagatg taatagcaga agctcaaact ggaacaggaa aaacactagc ttttttactt
      241 ccgatatttg aaaatatatc attagatata aatgatatac aagtacttat actttctcct
      301 acaagagaac tagctattca aataacagaa gaagctatga aacttaaaga atctaaagat
      361 gtaagtattc ttgcagcata tggaggaaaa gatattggtt ctcaaataaa gaaactaaaa
      421 ggaaatattc atatgattat agctacacca ggtaggcttt tagaccatct aaatagaaaa
      481 acaatagacc ttagtaagtt aaagactttt gttttagatg aagcagacca aatgttgctt
      541 atgggattta aaaatgaagt ggaagctatt ttaaaagaga cttcaaacaa aaagcaaaca
      601 ctatgctttt ctgcaactat aaattctcaa gtaaaaaaat tagcttatag atacacaaaa
      661 aatcctgttg tagtttctat tcaaaaagaa gaaatcactc ttaataatat aaaacaagaa
      721 gtagtagaaa ctacagatag aaaaaaatta gatgccttat gtaaggtact agatgaagat
      781 aatcctttta tggcaataat attttgtaga actaagagaa gggttgacaa tcttgaagaa
      841 gctcttgcta taagaggata taattgtcaa aaacttcata gtgatattgc tcaatcaaaa
      901 cgagaaagaa taatgaaatc atttagaaat ttagatatac aatacttaat agctacagat
      961 gtagcttctc gtggtcttga tatatctgga gtaagtcata tatataatta cgatttacca
     1021 gaaacaccag aagactacat ccaccgtatt ggtagaactg gtagagctgg tgaagaaggc
     1081 tatacatgtg cttttattga ccctaaaaat gaacgaatgt taagtgaaat agagacggct
     1141 atagaatcta aaattagtag aagaattata gaattataat ttatagattg cttaaaatac
     1201 caatgccaca caaacactgt aatatataga aagctacaga aaacatgtat caatctatac
     1261 tttgctgtta tactatttta tattaaagaa aaaagaggct atccaaagta ggattagtct
     1321 cttttattgt caaaataatg aaaatatatt tgacttcata taaaaaagag tgccttataa
     1381 actaaaagtc cattttgaga taccctcttt ttatctttta ttcttagttt tatattgctt
     1441 gtttatagtt gtttaatatt agattttgca aataatctta ataaacatgc agatatatta
     1501 tctatagtat tcttatttat agtattctct ttagcaatat tttttgctac tacatctaat
     1561 ttacattttc atttaattcc gctggccatt tagcttctgg attttccttt gcaaacttct
     1621 tattcttata atgtcctagt gtaagaaatg gaattactat agtgaataga ccaagataac
     1681 cacaatatcc atatccatat ttaacaatat tacttaatcc tgtaagtgat ataagcattg
     1741 acactaccat tattgcacaa gctgtaaata cccttctttt cattcttgaa gaaaggaaag
     1801 aaagtgcttt atggtcttca aatctattta caagaccaaa tatagtagtt actcctgtag
     1861 acattaaaca aaggaataat gcaacattat aaattatgta tatagtatga tttccagatt
     1921 gacctgcaac ataaagagta gggagtgttg tttgcccagc agcaataatt tctctatacc
     1981 atcccattag catagttaca gcaagtccta atgcaattgc attcataaca aatgaaatta
     2041 tcattgcctt agtagcttct tttgagttct taagaagtgg tcttgaacat cctgcaagtg
     2101 taggtataac aaccgattga aaacctgcat aaataaaagt attaagtatt ggcttgatat
     2161 agccaggtgt gaaactcgtt ctatttgata atatttcagt tatttctggt actttagctt
     2221 ttataccaac aataaaaata gtaagagtac atactagaat agctattgtc attgctgtag
     2281 ctgctttaga tacaagcata gcaccaaata tagtaaagac aagaagtaca actccaataa
     2341 taaatacagc tacaaaataa tttactccta tactttgaaa tactgctgct gcacctgcta
     2401 taacagcact tacagctatt aaaaccatta gatagtaata tatctcccat attatttcaa
     2461 gttttggata tggtctccaa agctcacaaa ataaatcctt ataattccta caattgttat
     2521 tattatacat tatgataact tctctcatgg tagcagtaag tataatcatt gctaaaattg
     2581 ccataagtgg tgctgtccat ccatactgca catagtactg agtttcttga ttccctgtag
     2641 caaatcctcc tcctgcatgt gaactaaaaa gaacgcttgc aacaccaaaa atagctaaca
     2701 tagagcccgt tacttttgct ttctttacac ttttttgcat gaaatcatcc tccctaaaat
     2761 gatattgaaa gtttttattt taacacttta ttgataaaac aataacattt ttgctcatta
     2821 aattcaacta tatttttcca atgactttac attttcaact tatttccacc tttatattaa
     2881 atatttttta tatatttatt ttatttttta aatagtattc aaattcactt tgttttttaa
     2941 ttttatcaaa tatctttttt aatgttaaat attatacatt ttttctt
//
LOCUS       DAYAPA010000225         2985 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.230, whole genome shotgun sequence.
ACCESSION   DAYAPA010000225 DAYAPA010000000
VERSION     DAYAPA010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2985
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.230"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(510..1406)
                     /locus_tag="ACGI59_003753"
     CDS             complement(510..1406)
                     /locus_tag="ACGI59_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFG9741499.1"
                     /translation="MKDKNTYFNRKGQENFIDIFLKDDIKKGANFKRHWHEHLQIYYF
                     IDGIAYLECGLDNFKVVPGNIVIANSNELHYLESLSDNLKFYSIRIEPTFLFSNQVDL
                     LQTKYLAPLALNHIVFNNLIQNDSLILKCITTILDEYHNKEVGYELSIKANIYQLIVL
                     LLRSHIDKIYSEDEFIKRKYSLEKFECIFDLIEEKYMDKITLKELASHVNLSAHHFCR
                     TFKQITGKTTIDFINGIRLDKAIYYLKETDLNMTEIAMRCGFDSINYFSRLFKRNYNI
                     SPTKYRNEHKEVSNVILKEDMK"
     gene            1547..2974
                     /locus_tag="ACGI59_003754"
     CDS             1547..2974
                     /locus_tag="ACGI59_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFG9741500.1"
                     /translation="MTNSFKKDDNRKKITVLFVVIAMTFMATLDSSIINVALPVLASK
                     LNVSLASIEWVIASYSIIICSTLLFFGRLGDIIGKSRVFQVGTILFTSASLLCGLSNS
                     LTLLIVCRFIQGIGASAYMANNHGIITELFPKESRGKALGILVTAVAIGNMVGPSVGG
                     FILSIFDWNVIFFINIPIGLIVIFLNTKFLPNSKKSSENMDKTGAILQFLGTTLFFSA
                     LISAQQTGLLNPYILIALLLSIIFIILFLILEKKHPQPLLDLEIFRNFKFSLNLICAL
                     TSFICIASSSILIPFYLQSTMKLPPIQAGLFMILSPLILAIFSPIFGNISDKIKSEKI
                     ILIGLLVMSFGFFLMSRLKESSALILFVIYISIISIGQAIFQPANNALIMSSCSRSKL
                     GVVGSINSLVRNLGQVIGITISTTLLYNFMSIKAGYRVNDYVINNDKIFVFGMRNVYI
                     IVTLVCLIGAILIGFYLFKYNKNEQ"
BASE COUNT         1024 a          396 c          339 g         1226 t
ORIGIN      
        1 ttttattcta cagttgcaat aaaaaactcg taataataac aaaaaaatct attattgtag
       61 aatcttgtat gttttttcta tttttatata tttttcagga tgatttttat tataaaattt
      121 tttataaata tataattttt ttatatttat ttgactttct tttaattctt taggatggat
      181 ttaaattttc tataaaaatc atactatttt agctgtttac ttgcttattt aatagtttta
      241 tttctttgat taaatatctt tcagaatcta agccagatgt ctcaaaaaaa ctgatttata
      301 atataatcat tttttctgct attcttattt ttccaatttt agtttcatat taaaatatac
      361 ttttcataga cactacattc tttcatactc attttcacat ttaactatac aagtagtctt
      421 actcatttat gtactatact ttattaatat ttattaagaa aaactcaaag atttaaaaat
      481 atatattttc tataaagtat atttatgaat tatttcatat cttcttttaa aataacatta
      541 ctaacttctt tatgttcatt tctatactta gttggagata tattataatt ccttttaaat
      601 aagcgactaa aataatttat gctatcaaag ccacatctca tagcaatttc tgtcatattt
      661 aaatctgttt cctttaaata atatatagct ttatcaagcc tgattccatt tataaaatcg
      721 attgtagtct tacctgttat ttgcttaaat gttctacaaa agtgatgtgc actcaagttc
      781 acatgactag caagttcttt taatgtaatt ttatccatat atttttcttc tatcaaatca
      841 aagatacatt caaatttttc taaagaatac tttcttttta taaattcatc ttcagaatat
      901 attttatcaa tatgacttct taataagagt actattaatt gatagatatt agcttttata
      961 gataactcat atccaacttc tttattgtga tattcatcaa gtatagttgt aatacatttt
     1021 aaaatcagag aatcattttg aattaaatta ttaaatacaa tatgatttaa agccaatggt
     1081 gctaagtact tggtttgtaa caaatcaact tgattactaa aaaggaatgt gggttcaata
     1141 cgaatactat agaattttaa attatctgat aagctttcta aataatgtaa ttcattactg
     1201 tttgctatta caatatttcc aggcactacc ttaaaattat ccaatccaca ttcaagatat
     1261 gctataccat caataaaata atatatttgt agatgctcat gccaatgtct tttaaagttt
     1321 gctccttttt taatgtcatc ctttaaaaaa atatctataa agttttcttg acctttcctg
     1381 ttaaaatatg tatttttatc cttcattatt cccccctcat caaaatagtg ctaattataa
     1441 gcaatatagt tattgtattc attatttcat atcgctatta tataaaatgt aactatcatt
     1501 gtaaacatta acataatgca gtcattataa taaggaggta ataaaaatga caaactcttt
     1561 taaaaaagat gataatagaa aaaaaatcac agttttattt gtagtcattg ccatgacttt
     1621 tatggcaaca ttagattcta gtattataaa tgttgctctc ccagttcttg caagcaaact
     1681 aaatgtatct ctagcatcta ttgaatgggt aatagcaagt tactcaatta ttatttgttc
     1741 aacactatta ttttttggaa gacttggaga tattattggt aaatcaagag tatttcaagt
     1801 aggtactatt ctctttacat cagcttcatt actatgtgga ttaagtaatt ctttaacttt
     1861 acttatagtt tgccgcttta ttcaaggaat aggcgcttct gcctacatgg caaacaatca
     1921 tggaattatc acagaactat ttccaaaaga aagcagagga aaagccctag ggattttagt
     1981 tactgcagtt gctattggaa atatggttgg accatctgtg ggaggattta ttttgtcaat
     2041 atttgattgg aatgttatat tttttataaa tattccaatc ggtcttattg taatcttttt
     2101 aaatacaaaa tttcttccta atagtaaaaa atcttcagaa aatatggata aaactggtgc
     2161 tatacttcaa tttttaggaa caacattatt ttttagtgct ctaattagtg ctcaacaaac
     2221 aggattacta aatccatata tattaattgc actattatta tcaattattt ttattatatt
     2281 atttttgata ttagaaaaaa aacatcctca accgcttctt gaccttgaaa tttttagaaa
     2341 ttttaaattt tcattgaatt taatttgtgc tcttacatca ttcatttgta ttgcatcatc
     2401 aagtatttta attccatttt atttacaaag tactatgaaa cttcccccta ttcaagcagg
     2461 tttatttatg atactatcac ctttgatttt agccattttt tcacctatat ttgggaatat
     2521 atcagataaa attaagtcag aaaaaattat tttaattggt cttctcgtta tgagttttgg
     2581 attctttcta atgtcaagat tgaaagaaag ttctgccctt attttatttg taatttatat
     2641 atcgataata tcaataggac aagctatatt tcaacctgct aacaatgctt tgataatgtc
     2701 aagctgttct agaagtaagc taggagttgt aggaagcata aattcccttg taagaaattt
     2761 aggacaagtt ataggtatta ctatttctac aacactttta tataatttta tgagtattaa
     2821 agctggatat agggtcaatg attatgtgat taataatgat aaaatatttg tatttggaat
     2881 gagaaatgtt tatattattg ttacacttgt ttgcttaata ggtgcaatat taattggctt
     2941 ttatttattt aaatataata aaaatgaaca atagttttta taatt
//
LOCUS       DAYAPA010000226         2824 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.231, whole genome shotgun sequence.
ACCESSION   DAYAPA010000226 DAYAPA010000000
VERSION     DAYAPA010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2824
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.231"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            480..1355
                     /locus_tag="ACGI59_003755"
     CDS             480..1355
                     /locus_tag="ACGI59_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFG9741501.1"
                     /translation="MLVYYLYNLIFLEGGVITLKLKIVGSGGMSIIPSSFCKCKICEE
                     ARQKGGRYERLGPSLYIDDIKMLIDTPEDIAVACNRQKISEVKHISISHHDPDHVKGI
                     RIVEKIGCDFITGENNPIGFYALPEVIEDINRTNLDCLKYYGDVINCISINETSHVKI
                     NNFDIDLINNNPDRNITFYVIKENEKKVIYACCNPKPFTHSDMYFDADILIISLVSDD
                     GILNDGTKLKDTPFKDEIFTLDEIVEIKNYYRIKKIIVTHIDEMWGKSYGYYSELEKK
                     LDNIYFAYDGMEIIV"
     gene            complement(1665..2444)
                     /locus_tag="ACGI59_003756"
     CDS             complement(1665..2444)
                     /locus_tag="ACGI59_003756"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFG9741502.1"
                     /translation="MSYKKIAIITGANSGFGKEFLKLLINEEEITEIWAVARNKARLN
                     QLVDEFGSKVKIFSKDLSKIEEVKEIGTFLSKENVCIKYLINNAGFAKFCSYNDLSID
                     ESINMIDLNISAVVALGLICIPYMQKGSHIMNISSQASFQPLPYQNIYSSTKAFIRNY
                     TRALNVELKSKGINAIAVCPGWMSTNLFKRGIVVAEKGTKNFPGIVSPDVVAKKALKD
                     AKKNKDISVYGINTKSSHLLAKLLPQKMMMKVWLMQQHIKE"
BASE COUNT         1045 a          381 c          334 g         1064 t
ORIGIN      
        1 ttgattagga tattatctta atttgtcaga taaatcaatt ataagattat atactatcct
       61 tatactctag ttaaatatat tttaatcact aatataaatt agttttttta acaaaacaat
      121 aaaagagtaa agtacaaata attcctttgt actctactct ttttaacttt ctttgtacaa
      181 taatacaatg ataaatataa aaaattatat attccattct atagaaatat gctcaccaat
      241 aacatgtatg ttgttaatca atatattata tcaaattata tcaaaattac ataaacctta
      301 taggacaatc atatcttatt acaagaaaaa tgtcttatcc atattgaata agtcattcag
      361 ttcatataca aagtcaattt cattgagaaa catttaatat atgtttataa aaatttcata
      421 ctattctttc atatttacat gtgatataat ttattaactc ataattttgt taatcagttg
      481 tgcttgtata ttatttatat aatctaatat ttttagaagg tggtgtaata actttgaaat
      541 taaaaattgt tggtagtggc ggtatgtcaa ttattccaag ttcattttgt aaatgcaaaa
      601 tatgtgaaga agccaggcaa aaaggcggaa gatatgaacg attaggacca agtttatata
      661 ttgatgatat aaaaatgctg atagacacac ctgaagacat tgcagtagct tgtaacagac
      721 aaaaaatatc ggaagtaaaa catatttcaa tttctcacca tgacccagac catgttaagg
      781 gtattagaat tgtcgaaaaa ataggatgtg actttatcac aggagaaaat aatccaattg
      841 gcttttatgc gcttcctgaa gtaattgaag atataaaccg aacgaatctc gattgtctta
      901 aatattatgg tgatgttata aattgcattt ctattaatga aacttcgcat gtaaagataa
      961 ataattttga tattgatttg attaacaata acccagacag gaatataacc ttctatgtta
     1021 tcaaagagaa tgaaaaaaaa gtcatatatg cctgctgtaa tcccaagcca tttactcata
     1081 gtgatatgta ctttgatgct gatattttaa taataagtct agtttctgat gatggtatat
     1141 taaatgatgg tacaaaactt aaagacacac catttaaaga tgaaatattt actttagatg
     1201 agattgttga aattaaaaac tattatagaa ttaaaaaaat tattgttaca catattgacg
     1261 aaatgtgggg aaaatcatat ggttattaca gtgaattaga aaagaaactt gataacattt
     1321 attttgcata tgatggtatg gaaattatag tctaaatcat taagtaataa gcataatgca
     1381 aaataagaaa acttcaattt aacagtatag tcaaactatg tgatatcaac atatcgcagt
     1441 ttttacaaaa tatcatagta tttatcttta taaaatttca agtcctacat attttaatac
     1501 ttcaatatct aaataaagta aaatcaaaaa tatattttat atacattata gacggcttat
     1561 cgtcactaga taagccgttt tgtcacaatt attatttgct caacaaaact tctcataata
     1621 tataacagat tgaatctcca actgatactt aattaccaaa actcttattc cttaatatgt
     1681 tgttgcatta accaaacctt catcatcatt ttttgtggca atagttttgc taataaatga
     1741 ctggattttg tatttatacc atacacagaa atatccttat ttttctttgc atccttcaat
     1801 gcttttttgg caacaacgtc tggagacact atgcctggga aattttttgt ccctttttca
     1861 gctacaacta tacctctctt aaatagattt gtactcatcc aacctggaca tacagcaatt
     1921 gcatttatcc ctttactctt aagttcaaca tttaatgctc ttgtataatt tcttataaac
     1981 gctttagttg aactataaat attttgatat ggcaatggct ggaatgatgc ttgtgaagaa
     2041 atattcatga tatggcttcc cttttgcata tatggaatac atataagtcc caatgctaca
     2101 acagcactaa tatttaaatc aatcatatta atagattcat ctatacttaa atcattgtat
     2161 gaacagaatt ttgcaaaacc agcattgttt ataaggtatt ttatacatac attttcttta
     2221 cttaaaaatg taccaatttc ttttacttct tctatttttg ataaatcttt tgaaaaaatt
     2281 tttaccttac ttccaaattc atctactaat tgatttaatc tcgccttgtt tcttgcaact
     2341 gcccatattt ctgttatttc ttcttcattt attaataact ttagaaactc tttaccaaaa
     2401 ccactattag cacccgttat aattgcaatc ttcttataac tcataattat tctcctttac
     2461 cataagttta tacgctcaaa ctgtaaactt attttttaca aataaaacac cctctattca
     2521 gatgttatat tcatattatt tgagatatta tatttatatc attttcaata attgttatat
     2581 agcataaaaa atagtgttaa tattatagaa tcggtattga ttatatttaa aaagtagtgt
     2641 taattatatt atagaatcag tatttattat attataaaaa gtagtgttaa tattatagaa
     2701 tcgatattga ttatatttaa aaagtagtgt taattatatt gtagaatcag tattgattat
     2761 attgtaaaaa gtagtattaa taatattgta gaatcaatat tgattatatt taaaaaagta
     2821 gtgt
//
LOCUS       DAYAPA010000227         2779 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.232, whole genome shotgun sequence.
ACCESSION   DAYAPA010000227 DAYAPA010000000
VERSION     DAYAPA010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2779
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.232"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            <1..1088
                     /locus_tag="ACGI59_003757"
     CDS             <1..1088
                     /locus_tag="ACGI59_003757"
                     /inference="COORDINATES: protein motif:HMM:NF012403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HFG9741503.1"
                     /translation="DWVYHFKTYYPDMGIINGTKKLSEGIEYFKIVEVPFIVVINYEL
                     AFRRKELLDLKDFTLMLDESSMIQNEKAKRSKFVLSLNPKNVILLSGTPCSGKYEQLY
                     SQIKLLGWNISKDLYWKQYIDVEYKDIGGFPMKVVTGYKNVDRLKKKLRDYGAVFMMS
                     DEVFDLPKQIDNVMKISTTREYRKFKKDSIISISDDVELVGDTTLTKMLYERQLCSQY
                     NKFKLEAFGDLINSTGDRLIVFYNFNEELDRLIELSRDRPISIVNGSTKDLNNYEEHN
                     NSITFVQYQAGAMGLNLQKCNKIVYFSLTLSCELFMQSKKRIHRIGQENTCFYYYMIC
                     RNSVEENIYKSLQSGVDYTNNLFEKGN"
     gene            1090..1524
                     /locus_tag="ACGI59_003758"
     CDS             1090..1524
                     /locus_tag="ACGI59_003758"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9741504.1"
                     /translation="MIKCNEICDFKGENICCSECGDNETCSNICGLECSTCGKTIIAD
                     DAGLEEFRDNNYVLFKNIFNICEQKKSLEIEEKKMKEELKKSMELYNIKSFDNDILKM
                     IYIEASTTTTIDSKKLKEKYPEIYSDCSKISNKKAYIKISLK"
     gene            1533..1835
                     /locus_tag="ACGI59_003759"
     CDS             1533..1835
                     /locus_tag="ACGI59_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003500708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HFG9741505.1"
                     /translation="MSAEKQFENKVKKFLKEQGCWFIKYWGGSAYTKSGIPDLLVCCS
                     GKFIGIEVKGEKGKASELQKYNIKEIEKAGGIGIILYPKDFDRFKKMILEIKKEGE"
     gene            1836..>2779
                     /locus_tag="ACGI59_003760"
     CDS             1836..>2779
                     /locus_tag="ACGI59_003760"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HFG9741506.1"
                     /translation="MQFSHSRIGVFKNCPYKYKLQYIDKVKTILNADSNNALILGTSL
                     HTGIEKDIDAAVKEYYFSYPSITDLHINEVMKLDYLIPKVKEILPKGFNEVQITTSDF
                     IGFIDLLAPHVYMTDTNLNTNETTIYYEDEERVFDIYDFKYSNNIDRYLESEQLHLYK
                     YFYEKQHPNHEIKDLYYVFVPKVQIRQKKTENLHQFRQRLLNELDKSEIKIVKVEYEP
                     NKVINFLVDIKRCLENKEFEKNQNKLCNWCDYQEYCEKGIDYMLLPNSERRSVEGINK
                     KTLWIYGAPFSGKTTLANDFEKPLMLNTDGNIKFVDAP"
BASE COUNT         1171 a          250 c          492 g          866 t
ORIGIN      
        1 acgattgggt atatcacttt aaaacatatt atccagatat ggggataatc aatggtacaa
       61 agaaacttag tgaaggcata gagtatttta agattgttga agttccattt atagtagtaa
      121 ttaattatga attagctttt agaagaaaag agttattaga tttaaaagat tttactttaa
      181 tgttagatga atcatcaatg attcaaaatg aaaaagctaa aagaagtaag tttgtactaa
      241 gtttaaaccc taaaaatgtg atactacttt ctggtactcc atgtagtggt aaatatgagc
      301 aactatattc tcaaattaaa ctactagggt ggaatatatc aaaagattta tattggaagc
      361 aatatattga tgttgagtac aaagatattg gtggttttcc aatgaaggtt gtaacaggtt
      421 ataagaatgt tgacagatta aaaaagaagt taagagatta tggagcagta tttatgatgt
      481 cagatgaagt atttgactta ccaaaacaaa tagataatgt aatgaaaatt agtactacaa
      541 gagagtatag gaagtttaaa aaagattcaa ttataagtat ttcagatgat gttgaacttg
      601 ttggagatac tacactaact aaaatgttat atgaaagaca attatgtagt caatacaata
      661 aatttaaatt agaagctttt ggagacttaa taaactcaac aggtgacaga ttaatagtat
      721 tttacaattt taacgaagag ttagacagat taatagaatt atcaagagat agaccaatat
      781 caatagtaaa tggctctaca aaagatttaa ataattatga ggagcataat aactcaatta
      841 catttgtaca atatcaagca ggagctatgg gtttaaatct tcaaaagtgt aataagattg
      901 tatattttag tcttacttta tcttgtgagt tatttatgca aagcaaaaag agaattcata
      961 gaataggtca agaaaacaca tgtttttatt attatatgat ttgtaggaat agtgttgaag
     1021 aaaatatata taaaagctta caaagtggtg ttgactatac aaataatttg tttgagaaag
     1081 gaaattaaaa tgattaagtg taatgaaatt tgtgatttta aaggtgaaaa tatttgttgt
     1141 agtgaatgtg gcgataacga gacttgtagt aatatatgtg gcttagaatg tagtacatgt
     1201 gggaagacaa ttattgcaga tgatgcagga ttagaggaat tcagagataa taactatgtt
     1261 ttatttaaaa atatatttaa tatatgtgaa caaaagaaaa gccttgagat agaagaaaag
     1321 aaaatgaaag aagagttaaa aaaatcaatg gaactctata atataaaatc atttgataat
     1381 gatattttaa aaatgatata tattgaagca agtaccacaa ctactattga tagtaagaaa
     1441 cttaaagaaa aatatccaga aatttattca gattgttcta agatatcaaa taaaaaggca
     1501 tatataaaaa tatctttaaa gtaggtggtt atatgagtgc agaaaaacaa tttgaaaata
     1561 aagttaaaaa gtttttaaaa gaacaaggtt gttggtttat taaatattgg ggaggttcag
     1621 catatacaaa aagtggtata ccagatttat tagtttgttg tagtggtaaa tttataggta
     1681 ttgaagtcaa gggagaaaaa ggaaaagcaa gcgaactaca aaagtataat attaaagaaa
     1741 ttgaaaaggc aggtggaata ggaattattc tataccctaa agactttgat agatttaaaa
     1801 agatgatttt agaaataaag aaagaaggtg agtaaatgca gttctcacat agtaggatag
     1861 gagtatttaa gaattgccca tataagtata agctacagta tattgacaag gtgaaaacaa
     1921 tattaaatgc agattctaac aatgctttaa tattaggaac atcacttcat actggtattg
     1981 aaaaagatat tgatgcagca gtaaaagaat attattttag ttatccaagt ataaccgatt
     2041 tacacattaa tgaagtgatg aagttagatt atttaatacc aaaagtaaaa gaaattttac
     2101 caaaagggtt taatgaggtt caaataacaa ctagtgattt tatagggttt atagatttgt
     2161 tagcaccaca tgtatacatg actgatacaa acttaaatac aaatgaaaca accatatatt
     2221 atgaagatga agaaagagta tttgatattt atgactttaa atattcaaac aacatagata
     2281 gatatttaga aagtgagcaa cttcatctat ataagtattt ttatgaaaag caacatccaa
     2341 atcatgagat aaaagatttg tactatgtat ttgtacctaa agttcagatt agacagaaga
     2401 aaacagaaaa cttacatcaa tttagacaaa gattacttaa tgagcttgat aaatctgaaa
     2461 taaaaatagt gaaggttgag tacgaaccaa ataaagttat taattttctt gtagatataa
     2521 aaagatgtct tgaaaacaag gaatttgaaa aaaatcaaaa taaattatgt aactggtgtg
     2581 actaccaaga atactgtgaa aaggggattg attatatgtt attaccaaat agtgaaagaa
     2641 gaagtgtgga aggaattaat aaaaaaactt tatggattta tggagcacca tttagtggca
     2701 aaactacatt agcaaatgat tttgaaaagc ctttaatgct taatacagat ggaaatataa
     2761 agtttgttga tgctccata
//
LOCUS       DAYAPA010000228         2754 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.233, whole genome shotgun sequence.
ACCESSION   DAYAPA010000228 DAYAPA010000000
VERSION     DAYAPA010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2754
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.233"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            248..1666
                     /locus_tag="ACGI59_003761"
     CDS             248..1666
                     /locus_tag="ACGI59_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HFG9741507.1"
                     /translation="MTELMIISGLVLLICITSSKVLYKFGVPILLIFILLGMLFGSDG
                     MVGIYFDNYELTKQLCSIGLIFIMFFGGFGTNWNMAKPVAIPSILMSSLGVIITAGVT
                     GMFCHMVIGTSLLEGLLIGAIAASTDAASVFAVLRSQKLNLKGSLASLLEVESGSNDP
                     IAYMLTLILLTLMNNSGVELIAPMVIKQIAFGLAIGFILAKLSVYILRHSNFEIEGFY
                     TIFITAIAILSYAFSEYLGGNGYLSVYISGIIIGNSRIPLKKSLVHFFDGISWIMQIM
                     LFFMLGLLSFPSKLPNVFGIAMAISIFMIVIARPLATFIVLAKFKFSNKEKLFISWVG
                     LRGAASVVFAIYAVTQGVAIENDIFHIIFFIALLSVGIQGSLIPTVARKLDLVDDNTP
                     VLKTFNDYDGEISSKLIEVNVDEGNKWANKSIIDSNIPDDILIVMIKRKEQVLIPKGS
                     TIIKTGDILVLSGDRIEELIQL"
     gene            1742..2554
                     /locus_tag="ACGI59_003762"
     CDS             1742..2554
                     /locus_tag="ACGI59_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFG9741508.1"
                     /translation="MFSIGMFSKINKITTKTLRYYEDIGLLKPEYVDEFTKYRYYTTE
                     QLPKLHQIITLKQMGLSLAEIKKAVENPEEVENILMNKEKEMLDVIKSEEYKIIKLRN
                     YLMNLKGEVNMNDVIIKSLPKVKVASMRKVLKGYDELFNLFPNVMAEEMMKTGCTCAV
                     PDYCFNIYHDGEYRETDMDVEMCQAINDLKEDTDILKFKELDAVENAVCILHNGPYST
                     LGKTYADAFKWIEDNKYKVVGNPRESYIDGIWNKESEEDWLTEVQIPVKRML"
BASE COUNT         1029 a          279 c          487 g          959 t
ORIGIN      
        1 atattaaaca atcaactgaa aaatgaaatg ttaataaaaa aaaattcaaa ttataaatat
       61 taaatgaata taattaaaac aaatatataa atttaagtat aacactcaca ttttaatgta
      121 ttgtgaaatt tttgttgaac tttatgtacc ataacttata aaatttatgt catgttatat
      181 ttgaataagt aataaaattg tagatgtaaa ttaatctaca aaatttatat gaaatggagt
      241 tgatgctatg acagaattaa tgattataag tgggttagta ctattgattt gtataacttc
      301 tagcaaggta ttgtataaat ttggagtacc aatattattg atttttatct tactagggat
      361 gttatttggg tctgatggga tggtaggaat ctattttgat aactatgaac tgacaaaaca
      421 attatgttct attggactaa tatttataat gttttttgga gggtttggaa caaactggaa
      481 tatggctaaa cctgttgcta taccttctat attaatgtct tcactgggtg taataattac
      541 ggctggggtt acaggtatgt tttgccacat ggtaatagga actagtttac ttgaaggttt
      601 attaattggg gcaattgcag cttcaacaga tgctgcatct gtgtttgctg tgttgcgttc
      661 tcaaaaacta aacttaaagg gttcacttgc atcattatta gaagttgaaa gtggaagtaa
      721 tgacccaata gcgtatatgc tgacactaat tcttttgaca ttgatgaata attctggggt
      781 agaacttatt gcaccaatgg taataaaaca aatagctttt gggttagcta taggatttat
      841 actagcaaaa ttatctgttt atattttaag acattcaaac ttcgagatag aaggatttta
      901 tactatattt ataacagcta ttgcaatact ttcttatgca tttagcgagt atcttggagg
      961 aaatggatat ttaagtgttt acatttcagg tattattata ggaaatagta ggatacctct
     1021 taaaaaaagt ttggttcatt tctttgatgg aatttcatgg attatgcaaa taatgttatt
     1081 ctttatgtta ggattgttat ctttcccttc taagttacca aatgtatttg gtatagcaat
     1141 ggccatatct atattcatga tagttattgc aagaccatta gcaacattta tagttctagc
     1201 aaaatttaaa ttctcaaata aagaaaaatt attcatatcg tgggtaggtc ttagaggtgc
     1261 ggcttcagta gtttttgcaa tttatgctgt tactcaagga gtagccatag agaatgacat
     1321 attccatata atatttttta tagcgttgct ttcagtagga atacaaggtt cattgatacc
     1381 aactgtagcc agaaagttgg atttagttga tgataataca cctgtattaa aaacatttaa
     1441 tgattatgat ggagaaatta gctctaaact tatcgaagta aatgttgatg agggtaataa
     1501 atgggctaat aagagtataa tagattctaa tataccagac gatatattaa tagttatgat
     1561 aaaaagaaag gaacaggttt taatacctaa aggttcaacc attataaaaa caggggatat
     1621 tttggtattg agtggagata gaattgaaga attaatacaa ttataattta ttttgtattg
     1681 actttaacct aaggggaaag tttaaactta aattaatcaa atctaatgcg caggtgatga
     1741 gatgttttct ataggaatgt tttctaaaat aaataaaata actacaaaga cattaaggta
     1801 ttatgaagat atagggttgt taaaaccaga atatgtagat gaatttacaa aatataggta
     1861 ttatacaaca gagcaattac ctaaattaca tcagataata actttaaaac aaatgggttt
     1921 atcattagct gagataaaaa aagctgtaga aaatcctgaa gaagttgaaa atattttaat
     1981 gaataaagaa aaagaaatgt tggatgtaat taaaagtgaa gaatataaaa taattaaact
     2041 acgtaattac cttatgaatt tgaaaggaga agtcaatatg aatgatgtaa ttattaagtc
     2101 tttaccaaaa gttaaagttg cttctatgag aaaagtgtta aaggggtatg atgaactttt
     2161 taatctattt ccaaatgtaa tggctgaaga aatgatgaaa acagggtgta catgtgcagt
     2221 tcctgattat tgttttaata tatatcatga tggtgaatat agagagactg atatggatgt
     2281 tgaaatgtgt caagctataa atgatttaaa ggaagatacg gatatattaa aatttaaaga
     2341 attagatgca gttgaaaatg cagtttgtat attacacaat ggtccatatt caacattagg
     2401 aaaaacttac gcagatgcat ttaaatggat agaggataat aaatataaag tggttggaaa
     2461 tcctagagaa tcttatatag atggtatatg gaataaagag agtgaagaag actggttgac
     2521 agaagttcag ataccagtga aacgcatgtt ataatatgtc tacaaagtat aaataagaat
     2581 aaaaaaggtg gtgtctcaaa gtaaacattt tagtttatga gacactcttt ttcatatgaa
     2641 atcaagtata tttttataat tacgattatg aaaaaaggct tatctatatt tttagacagc
     2701 ctcttatttt tttattataa tagttttcta aagatgctct atttataaat tttt
//
LOCUS       DAYAPA010000229         2743 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.234, whole genome shotgun sequence.
ACCESSION   DAYAPA010000229 DAYAPA010000000
VERSION     DAYAPA010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2743
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.234"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            388..2523
                     /gene="rnr"
                     /locus_tag="ACGI59_003763"
     CDS             388..2523
                     /gene="rnr"
                     /locus_tag="ACGI59_003763"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFG9741509.1"
                     /translation="MIPGLKESLLGLINESAYNPLKKEELAEIFDIHSSEMPMFYNFL
                     NELEEDGYICFTKKGKIVSPNQMGYFVGKFVSHKKGFGFVESDVEYTQDLFISSDNIN
                     GAMHNDRVMAEIVVPATEEKRAEGRIVKVIKREITRIVGTFQSSKTFGFVTPDNKKFT
                     KDIYIPKKHFSGAVDNDKVVCEITVWPQEDRKPEGKIIEILGQKGERGVEIDSIIREH
                     GLPEEFPKKVLQEAEAVAVEIPQEEIKRRRDLRDLNIFTIDGDDAKDLDDAISIEVLP
                     NGNFKLGVHIADVTHYVREKNKLDKEALKRATSVYLVDKVIPMLPKTLSNGVCSLNPF
                     EDKLTLSIFMEIDHKGNVVKHEICESVINSKARMTYTEVSDILEKDDEKLKKTFEHVV
                     EDFKNAEILARILMSRREKRGAIDFNFPEAKIILNGKGEVVDIKPYERRISNKIIEEF
                     MLISNETIAEHFYWMGIPFVYRIHETPSLEKMEELSKFISTFGYIIKGDKEEVHPKAL
                     QGIIEKIKGKKEEGAISTIMLRSLKQAKYSPECSGHFGLAAKFYCHFTSPIRRYPDLQ
                     IHRIIKESLNNKISGKRQEQLTTIVDYASVQSSEKERKAELAERDVHDFYKALYMKDK
                     VGQEFEGVVSSVTSFGIFVELDNTIEGLIRLANMNDDYYIYDESSYSVLGERTKKSYK
                     IGDLVKIKVESVNVDFKEIDFEIVEKIEE"
BASE COUNT         1187 a          262 c          497 g          797 t
ORIGIN      
        1 aaaaattaat tgagttatat aaattttaca tgatttgagt atactaatag aataaattat
       61 tatatagtaa tacttatatg agatgaatat tttaccaatc ctatttgatg gtgtataatt
      121 aggtgataaa gagtaattta agcaattatg ataaagaata acttaagtaa ttataatgat
      181 atttacaaat aatttataaa acatcataat ttgtttgaag ttagtatgac aaagcttaca
      241 ttaaatcaaa aatataaata agtaatttta acttataata ataagaaaat atagaaaatt
      301 atgtgataat taaaaaatgt tttgtctgaa gaaatataaa agacaatata aataataata
      361 taatgaaaag taaaacagga gggaaaaatg ataccaggac ttaaagaaag tttgctagga
      421 ttaataaatg aaagtgcata taatcctcta aaaaaagaag aattagcaga aatatttgat
      481 atacattcat cagaaatgcc aatgttttat aattttttaa atgaattaga agaagatggt
      541 tatatctgct ttactaaaaa aggaaaaata gtatctccca atcaaatggg atactttgtt
      601 ggaaaatttg tatcacacaa aaagggtttt ggatttgtag aatctgatgt tgaatataca
      661 caggatttat ttatctcaag tgacaatata aatggtgcta tgcataatga tagagttatg
      721 gcagaaatag tagtacctgc aactgaagaa aagagagcag aaggaagaat agtaaaagta
      781 ataaagagag aaataactag aatagtaggt actttccaat caagtaaaac ttttggattt
      841 gtaacaccag acaacaagaa atttactaaa gatatatata tacctaaaaa acattttagt
      901 ggagcagtag ataatgataa ggtagtatgt gaaattacag tatggccaca agaggataga
      961 aaaccagaag gcaaaattat agagatacta ggtcaaaaag gtgaaagagg cgtagaaata
     1021 gattctataa taagagaaca tggtcttcca gaagaatttc caaagaaagt attgcaagaa
     1081 gcagaagctg tagctgttga gattcctcaa gaagagataa aaagaagaag agatttaaga
     1141 gatttaaaca tatttactat agatggagat gacgcaaaag acttagatga tgccatatca
     1201 atagaagtat tacctaatgg aaacttcaaa ctaggagttc acatagctga tgttacacat
     1261 tatgtaagag agaaaaataa acttgacaag gaagccttaa aaagagctac atcagtttat
     1321 ttagtagata aagtaattcc aatgttgcca aaaactcttt caaatggagt atgtagctta
     1381 aatccatttg aagataaatt aactctttct atatttatgg aaatagacca taaaggaaat
     1441 gtggttaaac atgaaatatg tgaatctgta ataaattcaa aagctagaat gacttataca
     1501 gaagtatctg atatattaga aaaggatgat gaaaaactta aaaagacttt tgaacatgtt
     1561 gttgaagatt ttaaaaatgc tgaaatttta gctaggattc ttatgtcaag aagggaaaaa
     1621 agaggagcta tagacttcaa tttcccagag gcaaagataa ttctaaatgg aaaaggcgaa
     1681 gttgtagata taaagccata tgaaagaaga atatctaata agataataga agaatttatg
     1741 cttataagta atgagacaat tgccgaacat ttttactgga tgggaatacc gtttgtgtat
     1801 agaatacatg agacaccatc tttggaaaaa atggaagaat tgagtaagtt tatatctaca
     1861 tttggatata taataaaagg tgataaagag gaagtacatc caaaagcatt acaaggtata
     1921 atagagaaaa taaagggcaa aaaagaagaa ggagctatca gtactataat gcttcgttct
     1981 ttaaaacaag ctaagtactc accagaatgt agtggacatt ttggattagc agctaaattc
     2041 tactgtcact ttacttcacc aataagaaga tatcctgacc ttcaaattca tagaataata
     2101 aaagaatctt taaataataa aataagtgga aaaagacaag aacagcttac tactatagtg
     2161 gattatgcat ctgttcaatc atccgagaaa gaaagaaagg cagagttagc tgaaagagat
     2221 gtacatgatt tctataaggc tctttatatg aaggataaag ttggtcaaga atttgagggt
     2281 gttgtatcta gcgtaacttc atttggaata tttgttgagt tggataatac catagaagga
     2341 ttgataagac ttgcaaatat gaatgatgat tactatattt atgatgagag ttcttatagt
     2401 gttttaggag aaagaactaa gaagtcatat aagataggag accttgttaa gataaaggtt
     2461 gaaagtgtta atgtagattt taaagaaatt gactttgaga tagttgaaaa aatagaggag
     2521 tagataaaac agctaaaata agagataaca agttgtagta tttatgatat gtataaatat
     2581 atagtgcaca atactctata aaataaatga taaaaatagg taattgagaa atatctaatg
     2641 gaaatagtta actgaaagat aaataattaa gaaatattta atgaaagtag ctaattgaaa
     2701 aataagtaat tgagaaatgt ttaatagaaa gagctaactg aaa
//
LOCUS       DAYAPA010000230         2651 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.235, whole genome shotgun sequence.
ACCESSION   DAYAPA010000230 DAYAPA010000000
VERSION     DAYAPA010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2651
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.235"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            240..1859
                     /locus_tag="ACGI59_003764"
     CDS             240..1859
                     /locus_tag="ACGI59_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HFG9741510.1"
                     /translation="MNIVISLIGGLGLFLYGMSLMGEGLQKSAGDKLKKIIELLTSNV
                     VMGVLVGTVVTGIIQSSSATTVMVVGFVNAGIMNLSQAIGVIMGANIGTTVTAQLVSF
                     NLEGIAPIALGIGILFYLFTSNQKTKHLSEILIGFGILFTGMEFMKDAVAPLAEYKAF
                     TDALLYFSKNPVLGILAGFAITGIVQSSSASMGMLIALASQGILPLSSALPILYGDNI
                     GTCVTSLLSSVGASRNARRAAVMHLSFNVIGTIIFMLVLNKPISAIVTHFDPTDTARQ
                     IANAHTLFNLTNVIILLPFSKYIVKLANRLIPIKETESEIVNNTKYLDERMFSTPSIA
                     LGNTVQEVVRMGHKATNSLEHSIAGFLNKSNEDINKAFESEKVVNKLQKDILNYLLKL
                     SKEPLRDDERFRTDLLFNTVNDIERVSDHAENIAELAMSVKEMNISFSDSAIREIYEI
                     YNKTITNFKDALIVLDVKDFELANKVLEVENEVNYLEKTFRNSHMIRLNNGSCTIDAG
                     VLYLDLLTNLERISDHSTNIVKQVLKLKQKI"
     gene            2233..2427
                     /locus_tag="ACGI59_003765"
     CDS             2233..2427
                     /locus_tag="ACGI59_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFG9741511.1"
                     /translation="MINYMVEIIYSEKISEVENMLKIENIQYEFTSRDGFIFFGFYDM
                     TDAYRFETLCSDEKIRFKRI"
BASE COUNT          990 a          309 c          378 g          974 t
ORIGIN      
        1 ttgattgtac ataaaatact aaaaatctaa gttcaagtat ttaaattgct atatatcata
       61 aattttctaa aattaacaac aacttaaaaa tttaacattc tcttaacgta tacttaacaa
      121 aacattaata tttttattga caaaatttgt tattctatat tatggaaaca ataaatttta
      181 gttaataaag tttataattc agataaaaaa attattaatt tcttaaggga ggaaatatcg
      241 tgaatatagt aattagcctt atcggtggac ttggactttt tctttacgga atgagtttaa
      301 tgggagaagg tttacaaaaa tcagcaggag acaaattaaa aaaaattatt gaactcttaa
      361 caagtaatgt tgttatgggt gtactggtag gtacagtcgt tacaggtatt atacaaagtt
      421 caagtgctac tactgttatg gttgtaggat ttgtaaatgc aggtataatg aatctaagtc
      481 aagctattgg tgtaataatg ggggcaaata taggtactac agttactgct caattagttt
      541 catttaatct tgaaggaatt gcaccaattg ctttaggtat aggtatttta ttctatttat
      601 ttacatcaaa tcaaaaaaca aagcatttat ctgagatact tataggtttc ggtatattat
      661 ttacaggaat ggagtttatg aaagatgcag tagctccact tgctgaatat aaagcattca
      721 ctgatgcact tttatatttt agtaaaaatc ctgtattagg tatattggca ggatttgcca
      781 taactggtat tgttcaaagt tcaagtgcat ctatgggtat gttaatagca ctagcttctc
      841 aaggaatact tcctctatca tctgctttac ctattcttta tggagataat ataggtactt
      901 gtgttacttc acttttatca agtgttggtg caagtagaaa tgctcgacgt gcagctgtaa
      961 tgcatttaag ttttaatgta attggtacaa taatatttat gcttgtttta aataaaccta
     1021 tatcagccat agttactcat tttgacccaa ctgatacagc tagacaaata gcaaatgcac
     1081 atactttatt taatcttaca aatgttataa tattattacc attttctaaa tacatagtta
     1141 aattagcaaa tagacttata cctattaaag aaactgaatc agaaatagta aataatacta
     1201 agtacttaga tgaaaggatg tttagcactc catctatagc acttggtaat actgtacaag
     1261 aagttgtaag aatgggtcat aaagctacaa actctttgga acattcaatt gcaggatttt
     1321 taaataagag caatgaagat attaataaag cttttgagtc agaaaaagtt gtcaataagt
     1381 tacagaaaga tatattgaac tatttgttaa aactttctaa agaacctctt agagatgatg
     1441 aacgctttag aacagatttg ctttttaata ctgtaaatga cattgaaaga gtctctgacc
     1501 atgcagaaaa tatagctgaa ttggccatga gtgttaagga aatgaacatt agtttttctg
     1561 atagtgcgat aagagaaata tacgaaatat acaataagac aattactaat tttaaggatg
     1621 ccttaatagt tttagatgtt aaagattttg agttagcaaa taaagtttta gaagttgaaa
     1681 atgaagtaaa ttatttagaa aaaactttta gaaattcaca tatgattaga ctaaataatg
     1741 gaagttgtac aattgatgct ggtgtacttt atcttgactt acttactaat ctagaaagaa
     1801 tttctgacca ctcaacaaat atagtaaaac aagtactaaa acttaaacaa aaaatatagt
     1861 gattattttt atatataatc ttaatcaatg agaagtaaga agctatagat aatgcttata
     1921 tcatttatgg cttcttattt tttatgtaat actcaaattt ttatagtatt ctcttattat
     1981 taaacagttt ctgcctagaa tattccatat atatatgact tctttcataa aacttgaact
     2041 gcccataaat aaagtcacaa gtatacataa ctatttttta atcaattata accaatattt
     2101 gttttattta tcttcatttt aattcttcat tcaataatgt aataatatta ttttttattt
     2161 ggatactatt atatcatcta aatatgttat tatatatttg gatattttca tattaatagg
     2221 agggattcta caatgataaa ttatatggta gaaattatat atagcgaaaa aataagcgaa
     2281 gtagaaaaca tgttgaaaat tgaaaatata caatatgaat ttacttctcg tgatggcttt
     2341 atattttttg gtttttatga tatgacagac gcatatagat ttgagacttt atgttcagat
     2401 gaaaaaattc gatttaaaag aatataatag ataaaaaagt acatatttct aaactatttg
     2461 tttttcaaat ttaggttgtg ttttaatgta gtgttgatat tataaacaat tttaataata
     2521 aaaaagtagg gacattatat taatatgctc ctacttttta ctcatactta ttaatattat
     2581 ttatcaaaat taccctacaa actggaattt atcattcttt cataatttta aactatcata
     2641 tttaaaatat a
//
LOCUS       DAYAPA010000231         2528 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.236, whole genome shotgun sequence.
ACCESSION   DAYAPA010000231 DAYAPA010000000
VERSION     DAYAPA010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2528
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.236"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            177..515
                     /locus_tag="ACGI59_003766"
     CDS             177..515
                     /locus_tag="ACGI59_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3784 domain-containing protein"
                     /protein_id="HFG9741512.1"
                     /translation="MFAVGFVVIIALAFFILSVLLFNGKASMLLAGYNSMSKKEKARY
                     DKKKLCRSSSIVTFIVSIMLFIMAFLGYRIESGQMDEKKMVPFSIIFIIVILSSVFFN
                     ILYSNKKCKK"
     gene            complement(922..2358)
                     /gene="miaB"
                     /locus_tag="ACGI59_003767"
     CDS             complement(922..2358)
                     /gene="miaB"
                     /locus_tag="ACGI59_003767"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HFG9741513.1"
                     /translation="MSKRKQVTIPIEKIQEQDKYIEQIHRQNEEYFNRTGKRKLVFTQ
                     TFGCQMNEHDSEKLCSMLEEMGYQMSMMVEESDLIIYNTCAVRENAELKVYGNLGQLK
                     HLKGKNPDMKIAVCGCMMQQPHVVEELRKKYKHVDLIFGTHNLYKFPQLLTESINSDK
                     MLVDVWDVDGEVIEGLRSNRKFELKAFVNIMYGCNNFCTYCIVPYTRGRERSRTPEDI
                     INEIKELVANGTKEITLLGQNVDSYGKTLENPVSFSELLRKVNDIEGIERVRFMTSHP
                     KDISDEVIYAIRDCDKVCEFLHLPIQCGSSSLLKKMNRHYTKEYYLEIIEKAKKEVPG
                     IAFSTDLMIGFPGETEEDLLDTLDVVEKVRYDSAFTFIYSKRQGTPAAKMENQIPEDI
                     KHDRFNRVLEAVNRISAEINDGYKDRIVEVLVEGRSKNNENKFAGRTRQNKLVNFEGG
                     NDDLIGKLVMVKITEPRTFSLNGILVNN"
BASE COUNT          826 a          384 c          278 g         1040 t
ORIGIN      
        1 atttgatggt tttcaatgca atttcaacat aaatactata tattagtgta tactgttgat
       61 gaattaaagt atgataatct atttaataaa gtatgaagta aatgatttta atgtgccttt
      121 atggtaaaat tactaataag taataaaatt aattaaaata tatggaggtc cttataatgt
      181 ttgctgtagg atttgttgtt ataattgcac ttgccttttt cattttaagt gtactattat
      241 ttaatggaaa agccagtatg ctactagcag gttataactc tatgagtaag aaagaaaaag
      301 cacgatacga taaaaaaaaa ttatgtagaa gttctagcat tgttactttt attgtctcta
      361 taatgctatt tattatggcg tttttgggat atagaataga gagtggtcaa atggacgaaa
      421 aaaaaatggt tcccttttct ataatattta taatagtcat actttccagt gtatttttta
      481 atatcctcta ctctaacaaa aaatgtaaaa aataaatagt tgtaagttga gcagttaatt
      541 ataaaagatt gactgctttt tcatgtatat tgactactct tattaaatat tttaacattt
      601 aaaaatctat gtaaaaataa tgaatatagt acaaacttct aataatgatt ttacctttgt
      661 ggttacagtc cactatatat aatttaatat gataacaggg aattattaat gacaactaag
      721 taaactttta taaaatataa taaaacaaaa atagatgtac aatgaaatta tatcttatat
      781 caaatttatt tttattaata gcaaaaactt acattagcat aacatatgac aaattcgctt
      841 aaaataaaaa tttttaatat aatttgttct atagtacatc tattttgatt agtttacata
      901 attttattat tttgtacaat attaattatt tactaatatc ccatttaatg aaaaagttct
      961 aggttcagta attttgacca taactaattt accaattaaa tcatcatttc caccttcgaa
     1021 atttacgagt ttattttgtc tagttctacc agcaaactta ttttcattat ttttacttct
     1081 tccttcaact agaacttcaa ctattctatc tttatatcca tcatttattt ctgcagaaat
     1141 cctatttaca gcttctaaca ccctattaaa tctatcatgc tttatatctt ctggtatttg
     1201 attttccatt ttagcagcag gagttccctg tcttttagaa taaataaatg taaaagctga
     1261 atcatatcta actttttcaa ccacatctaa agtgtccaat aaatcttctt ctgtttctcc
     1321 tgggaaacca atcattaagt ctgtagaaaa agcaatacct ggaacttcct ttttagcttt
     1381 ttctattatt tctaaataat attcctttgt ataatgtcta ttcatttttt tcaaaaggct
     1441 actgcttccg cattgtattg gtagatgtaa aaactcacac accttatcac aatctctaat
     1501 tgcgtatatt acttcatcag atatatcttt tggatgagat gtcataaatc gaaccctttc
     1561 aattccttct atatcattca cttttcttaa aagttctgag aatgaaactg gattttcaag
     1621 tgtcttacca taagaatcta cattttgtcc aagtaaagtg atttctttcg ttccatttgc
     1681 aacaagctct tttatctcgt taattatatc ttcaggagtt ctacttcttt ctcttcctct
     1741 tgtgtatggg actatacaat atgtgcagaa attattgcat ccatacatta tatttacaaa
     1801 tgcttttaat tcaaattttc tattacttct aagaccttct attacttctc catccacatc
     1861 ccatacatcc actaacattt tgtcagaatt tattgattct gtaagtaatt gcggaaattt
     1921 atatagatta tgtgttccaa atataaggtc tacatgttta tatttttttc ttaattcctc
     1981 tacaacatgt ggttgttgca tcatacatcc acatacagct attttcatat ctggattttt
     2041 tccttttaga tgttttaatt gtcctaagtt accatatact tttagctctg cattttctct
     2101 aactgcacag gtgttatata ttattaaatc actttcttca accatcattg acatttggta
     2161 tcccatctct tctaacattg aacataattt ttctgaatca tgttcattca tttgacaccc
     2221 aaaggtttga gtaaacacga gttttctctt tccagttctg ttgaagtatt cttcattttg
     2281 tctgtgtatt tgttctatat atttatcttg ttcttgtatt ttctctatag gtattgttac
     2341 ttgttttctt ttgctcacgt ctattcctcc tagttaattt cttatattac acataacctt
     2401 aattatatca aataagaaaa atcttttaaa gatattatta tttttctagt tgtgttttaa
     2461 tagtaaatct aatgatttat atatactata tttctaaaat ataaaatcat ataattatta
     2521 taataata
//
LOCUS       DAYAPA010000232         2508 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.237, whole genome shotgun sequence.
ACCESSION   DAYAPA010000232 DAYAPA010000000
VERSION     DAYAPA010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2508
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.237"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            326..1627
                     /locus_tag="ACGI59_003768"
     CDS             326..1627
                     /locus_tag="ACGI59_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFG9741514.1"
                     /translation="MGKSKDVEIEKSILGTILLDNKLSYKLDELNENMFMNDICLEIF
                     KIMKELKKENIVIDVATVKSKIDRKSLAIKTSDVTNLITWGQNFGLDGHIKILKENLA
                     RRSINQNCQNLLHSLNLGENIDTCIYKFESNIKEILDKDTYENDDVNSIAGKVLDFLE
                     NKKDIGFKFGIKLLDTTIGGLFKGELTTIAAKSGVGKTALALQIMLNSFKQGKKTLFI
                     SREMTSEQVFMRNICRVTGVSTRNMKSKEIDENDWKLIVNAIGDLSENNLIYINDKID
                     TISAIRKRIRQVKPDLLIVDYVQLLTSQKSMDKREREVATFSRELKNMTLDFNIPVIQ
                     LSQLNDEMKDSRPYGDRPMRDSKAIYHDSNNVVYIHQLKGSDYEEAVRDIGESEEAVR
                     ASEYRGIKMVDLIVAKCRDGQTRHKHFCYFGDKLHFQELNY"
     gene            1703..1978
                     /locus_tag="ACGI59_003769"
     CDS             1703..1978
                     /locus_tag="ACGI59_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mor transcription activator family protein"
                     /protein_id="HFG9741515.1"
                     /translation="MLELRKIIELLEEHKIEVSIPKELHKYSLLEYLELCQQSGGTYF
                     YIKTLEGALKEARNELMIKLFNGKNYMELARMFEMSVINVRHIVRKK"
BASE COUNT         1038 a          205 c          489 g          776 t
ORIGIN      
        1 tatcgagtaa tgaattatat tgaacgatat atcaataatt ggcagtaagt tatatcaaat
       61 aagatattac tatcgagtaa tgaattatat tgaacaatat attaataatt ggcagtaagt
      121 tatatcaaat aagatattgc tattatgtag taaattatat aaaacaatgt atcagaataa
      181 atagtatatt aataatttgt agtatatcat gacgaataat acagtcataa caaatagtga
      241 gtcataacaa ataatgtatt tataattgat aatgtattta aaaatcaaaa tataaaaact
      301 tataaggaaa gagagattaa tttatatggg taaatcaaaa gatgttgaaa tagagaagtc
      361 tattttaggc acaattcttt tagacaataa attaagctat aagctagatg aattaaatga
      421 aaacatgttt atgaatgata tatgtcttga gatatttaaa attatgaaag aacttaaaaa
      481 agaaaacata gtgattgatg tggccactgt gaaatcaaag atagatagaa aatctctagc
      541 aataaaaaca agtgatgtga ctaatctaat tacttggggt cagaattttg gtcttgatgg
      601 tcatattaaa attttaaaag aaaatttagc tagaagaagc ataaatcaaa actgccaaaa
      661 ccttttacac agcctaaacc ttggagaaaa tatcgatact tgtatttaca aatttgaaag
      721 taatataaag gagattttgg acaaggatac ttatgaaaat gatgatgtaa atagtatagc
      781 agggaaggtg cttgattttt tagaaaacaa aaaagatatt ggatttaaat ttggtataaa
      841 attacttgat accactatag gaggtctttt taaaggagag ctaaccacaa tagcagctaa
      901 atcgggggtt gggaagactg ctttggcact tcaaataatg ttaaattcat ttaagcaagg
      961 taaaaaaact ttatttataa gtagagaaat gaccagtgag caagtattta tgagaaatat
     1021 atgcagagta actggtgtaa gtacaaggaa tatgaaatct aaagaaatag atgaaaatga
     1081 ttggaaactt attgtaaatg cgataggtga tttaagcgaa aataacttaa tttatatcaa
     1141 tgataagata gatacaatta gtgcaattag aaagaggata aggcaggtaa agccagattt
     1201 attaatagtg gattatgtgc aactccttac atctcagaaa agcatggata agagagaaag
     1261 ggaggtagct actttttcga gagaacttaa aaatatgact ttagatttta atatacctgt
     1321 aatccaatta agtcagttaa atgatgaaat gaaagattca agaccttatg gagatagacc
     1381 tatgagggat agcaaagcca tctaccacga tagcaacaat gtggtttata tacatcaatt
     1441 aaaggggagt gattatgaag aagcagtgag agatattgga gaaagtgagg aggcagttag
     1501 agcatcagaa tacaggggaa ttaagatggt tgatttaata gttgcgaagt gtagagatgg
     1561 acagactagg cataagcatt tttgttactt tggggataag ttacattttc aagaactaaa
     1621 ttattaggtc aataaagtag taataacaag cttattaaaa gtcgggaaat agcaaataga
     1681 ttataaaata tgaggtgaaa atatgttgga attaagaaaa attatagaat tacttgaaga
     1741 acacaagata gaagtaagta ttccaaagga gttacataag tatagtttat tggaatacct
     1801 tgaattgtgt caacaaagtg ggggaacata tttttatatt aaaacactgg aaggggcttt
     1861 aaaagaggct agaaatgagc ttatgattaa attgtttaat gggaaaaatt atatggaatt
     1921 agcgaggatg tttgaaatga gtgttattaa tgtcaggcat attgttagaa aaaaatagat
     1981 atttttggag aatacaagtg tgatatactt ctgaaataga agttattata agaggagtat
     2041 aagtgtgatt aacaacatga ttttgtgaat aaaattgagg atatatatga gttaaatgtg
     2101 tgattaataa ggtaagattg tgtataagat tatgagtatt taaaaggagg ggctataatt
     2161 aatagtgtag agttgtgtgt ttgcttaaga ttatataata atttaataga ttaaactata
     2221 ctaataaggt aaaatcgtgt gtttagttaa agcttattat aaaatcttga tatgtgatta
     2281 atgatgcaat atgtgaataa agttaagatt ataatagatt taacctgtgg ctaatagaat
     2341 aaaattgtga ataaagttaa gattataata ggtttaaatt gtgactaata gagtaagagt
     2401 gtaaatagag ataaacttca atatgagctt aacctatgat taatagagta agaatgtaaa
     2461 tagagataaa cttcaatatg agtttaactt gtggctaata gagtaaga
//
LOCUS       DAYAPA010000233         2457 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.238, whole genome shotgun sequence.
ACCESSION   DAYAPA010000233 DAYAPA010000000
VERSION     DAYAPA010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.238"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            950..2305
                     /locus_tag="ACGI59_003770"
     CDS             950..2305
                     /locus_tag="ACGI59_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HFG9741516.1"
                     /translation="MSSTVKKKGKFPMGFYICSTTFSFERAAYYASKYLIYIFLTTAI
                     VHGGLGIDKGQAAIMQANLVAFTYLAPIIGGYISDRWIGARYTIPIGMLIMGVGYYLG
                     SIATTVSMVNAMIILVSIGTAFFKGNVSAVNGQLFDSQEELDTAFSVQYSFVNIGSFI
                     GTIAVGILYLKTFAKNGVLGFSQCFFIAAVLCVIGAIWFIYGWRFLGNAGKRPFKEGV
                     VAEKIEEKDKSPLTSMDKKRIWAIILISFFSVIFWVFWYLTYLAVYDYGAAFVNMNVG
                     GFDVPLAWFDSLNSLVCIVLGPVLGALWFKLASRPQGDMSLFKKTGLGLIFLGLAFLM
                     LVGAEFSRGVGAPETAKASILWIIMFGILLSLGEMLFSPLGNSFVSKYAPKKLLGVLM
                     GVWTFATFIAGKGYGYIYAFTLKFDMIKVYTIIPVILFVAAILLFLSDKTLSRLVEDD
                     K"
BASE COUNT          875 a          261 c          410 g          911 t
ORIGIN      
        1 gattattata tactgcatac aaaaatatat ttgagcaaat aagtaaagta ataaaaaaat
       61 caagatttca tatctaaaaa ctattctgaa cattaattta atttatattt tcataggaaa
      121 tattttatat aatataattg ataaatataa tttgaatttt tctaattatt tactgtcgat
      181 taatagattt ttttcttgca tataagtgca tatttatggt acgatttaca tggtgaaata
      241 aaaaagggga ggtgaaaaat aaatattcgt gaaaaagttt tcagtttgag gtattttatg
      301 aataatatat tttgaggtat tttataaata atatattata cataaagagt atagcctcaa
      361 taaatagtat caaagcgaat atatgggtaa ttactttaaa ataacagttt ttatgtttag
      421 attcacagtg tttattataa actaatgtgt aagaaataag cctaatctgt gaatagatag
      481 tagtttagtt atttaagttg agaattggtt gggtgaagta atatataaat ttaaaaaata
      541 aataaaaaca ttatttgaac aaaatatatg ttgctttatt cgagactcct ttaaaaaagc
      601 actaaaatgt aattacataa gttgctacaa aagggggatt ttagtttgta aaatactatc
      661 acgtaaaaaa taagaattgc atatggcgaa ggttttttca tggtattaga tgagaatata
      721 ttaatttata actatatctg aaaatagtga atttatataa tttaaattta tgattaagaa
      781 gtgtattaat tttattccat gagaatatgt actctacaaa taaatgcgca attatcttaa
      841 taataattac aaattgtttt acaaaataat aatattatat ctttcataaa agaaagaatt
      901 tggacaaaac gtaaaaaata aaaatacaat taagaaagga tttaaaatta tgagctcaac
      961 agtaaaaaag aagggtaaat ttcctatggg attttatatc tgctcaacaa ctttctcatt
     1021 tgaaagagca gcatattatg cttcaaagta cttaatatat atattcctta caacagctat
     1081 agttcatggt ggtcttggaa tagataaagg acaagctgct ataatgcaag ccaaccttgt
     1141 tgcattcaca taccttgcac ctataattgg ggggtatata tcagatagat ggataggagc
     1201 tagatatact atacctatag gtatgcttat catgggggta ggatactatc ttggttctat
     1261 tgcaactaca gtttctatgg taaatgcaat gataatctta gtttcaattg gtactgcatt
     1321 ctttaaaggt aacgtatctg cggtaaatgg acagcttttt gatagtcaag aagaacttga
     1381 tacagcattt tcagtacaat attcttttgt taatataggt tcttttattg gtactatagc
     1441 agttggtatt ctttacctta aaacatttgc aaaaaatggt gtacttggat tttctcaatg
     1501 tttctttata gcagctgtac tttgtgtaat aggtgcaata tggttcatat atggatggag
     1561 attccttggg aatgcaggaa agagaccttt taaagaaggt gtagttgctg aaaaaattga
     1621 agaaaaagat aagtctcctc ttacaagcat ggataagaag agaatttggg ctattatact
     1681 tatatcattc ttctctgtaa ttttctgggt attctggtat ctaacttatc ttgcagttta
     1741 tgattatggt gcagcatttg taaacatgaa tgttggagga tttgatgttc ctcttgcttg
     1801 gtttgactca ttaaattcac ttgtatgtat agtattagga ccagttcttg gagcattatg
     1861 gtttaaactt gctagtagac cacaaggaga tatgagttta tttaaaaaga caggtcttgg
     1921 attaatattc ttaggtcttg cattcttaat gcttgttggt gcagaatttt caagaggagt
     1981 tggagctcct gaaactgcta aagctagtat actatggata atcatgtttg gtattctttt
     2041 aagtcttggt gaaatgttat tctcaccact tggtaactct tttgtaagta agtatgcacc
     2101 taagaagctt ctaggtgttc ttatgggagt atggacattt gctactttta tagctggtaa
     2161 aggatatgga tatatatatg catttactct taaatttgat atgattaaag tatatactat
     2221 aattccagta atactatttg ttgcagcgat attattattc ttatctgata agacattaag
     2281 tagacttgtt gaggacgata aataatcagt aagcaattat tatgttttat aggttaatct
     2341 actgtaatta tgttgtatta gattgattta taactaatta ataataaaaa taaaatttta
     2401 cttctaaata caatattatg atatatataa ttataatatt tgttagagta tgattat
//
LOCUS       DAYAPA010000234         2446 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.239, whole genome shotgun sequence.
ACCESSION   DAYAPA010000234 DAYAPA010000000
VERSION     DAYAPA010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.239"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            <1..479
                     /locus_tag="ACGI59_003771"
     CDS             <1..479
                     /locus_tag="ACGI59_003771"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFG9741517.1"
                     /translation="IMFKEYLIRELKEDDIKNALDLIWNVFEEFESPDYCDEGITTFH
                     QFIEYNSIIEKIKEKTLFFWGCFDSKKLIGVLAIRNLNHICMLFVDKNYHKHGVAKKL
                     MTTGIQYCICNKFNLTEITVNSSPYAVEVYHKLGFKDTAVEQIVDGIRFTPMKLLI"
     gene            complement(848..1405)
                     /locus_tag="ACGI59_003772"
     CDS             complement(848..1405)
                     /locus_tag="ACGI59_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFG9741518.1"
                     /translation="MRVSREIVIQATSDIVNKDGLNKVSLKVIAEKLGIRTPSLYNHI
                     ESLDDLLLEVAHKGMREMNSQMIKATIGNFGDTAIKCISIAYFNFIISNPGVYETIQW
                     ATWHGNNETLEIFDEYKSLLEKLILSCNLNTKNTGEVLNLIMSVLHGYSTLQLGKSLL
                     SKEEAIKGLADSIDTVLLGIHKKYD"
     gene            1663..2361
                     /locus_tag="ACGI59_003773"
     CDS             1663..2361
                     /locus_tag="ACGI59_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFG9741519.1"
                     /translation="MEKKYFEIHGVPSILWGNPSSKLYIYIHGQCGNKEEAEAFANIA
                     VPHECQVLSVDLPEHGERKREKNSFNPWHVVPELVSIMEYAKCHWNQISLYANSIGAW
                     FSMLSFEKENLRECIFVSPILDMQQLISNMMLWANVSEEQLKHELIIPTSFGHTLSWE
                     YLQYVKQHPITQWNVPTKILYGKHDELTEIGVVEKFVEKFNCNLTIIENGKHWFNTPQ
                     ELNSLYEWLNTLVK"
BASE COUNT          902 a          322 c          298 g          924 t
ORIGIN      
        1 ttataatgtt caaagaatac ttaataagag aattaaaaga agatgatatt aaaaatgcat
       61 tagacttaat ctggaacgta tttgaagaat ttgaatctcc agattattgt gatgaaggaa
      121 ttacgacatt tcatcaattt atagaatata actctattat tgaaaaaatc aaagaaaaaa
      181 ctctcttctt ttggggttgt tttgactcta aaaaacttat tggagtctta gctattagaa
      241 atttaaatca tatatgtatg ttatttgtag ataaaaacta tcataaacat ggtgttgcaa
      301 aaaaattaat gactacaggc atacaatact gtatttgtaa taaatttaat ttaactgaaa
      361 taactgtaaa ttcatcacct tatgctgttg aagtttatca taaattagga tttaaagata
      421 ctgctgttga gcaaattgtt gacggtatac gttttacacc tatgaaattg ttaatataat
      481 aaattaaata aaaagcaaag tctcttaaat attttatagt tactttgctt ttaaaccaag
      541 atttaaaata atactatctt ttttgttttt taataatata ctttaaaatc cttactaata
      601 tgaactataa tttatttttt gatatccttt ttgattaaca tataaattaa ttcaattatt
      661 aaacataaac tatgttgttt ctctttatat ctcttctttg taaacaatat tgggcatttg
      721 aaaataaata ttcttaaaat atactgttct cctgttgatt ttcctttggt acaaatgata
      781 aaaatttaat ttacatgccc cataatgaag tgttcaataa ataagttaaa ataatttatt
      841 tacctcctta atcatatttt ttatgaatac ctaataacac agtatcaata gaatcggcta
      901 aaccttttat agcttcttct ttactcaaaa gtgatttccc aagttgcaaa gtcgaataac
      961 catgaagtac actcattatt aagtttaata cttcacctgt attctttgta tttagattac
     1021 aagaaagaat aagtttttct aataaggatt tatattcatc aaagatttct aatgtttcat
     1081 tatttccatg ccaagtagcc cactgaattg tttcataaac accaggatta gaaattataa
     1141 aattaaagta tgctatactt atacacttta tagctgtatc tccaaaattt ccaatagtcg
     1201 cctttatcat ttgactattc atctctctca ttcctttatg agctacttca agtaataaat
     1261 catctagact ttctatatga ttgtacaaag atggtgtacg tattcctagc ttttctgcaa
     1321 taacttttaa agaaactttg ttaagcccat ctttattaac tatatctgat gtagcttgta
     1381 taactatttc tctactaaca cgcatgattt tccctctttc atttaaaatt aattacttta
     1441 ttagtttagc taatctaatt agtttcgtca attattgtaa tttataaaat tctttatact
     1501 attataaaat cttgacagtt taattataat ttaatataat actaattgaa ttagttttaa
     1561 tactaatata attagtatta tattaaattc tatattacaa cattatttta tagtttgatt
     1621 aatttaacaa tcactaatat atcaaactga aaggagattt ttatggaaaa aaaatatttc
     1681 gaaattcatg gagttccttc aatcctttgg ggaaatcctt caagtaaatt atatatttac
     1741 atacatggac aatgtggcaa taaagaggag gctgaagctt ttgctaatat tgcagttcca
     1801 catgagtgtc aagttttaag tgttgatttg cctgaacatg gagaacgtaa aagagaaaaa
     1861 aattcattta acccatggca tgttgttcca gaattagtat ctattatgga atatgcaaag
     1921 tgccactgga atcaaatttc gttatatgca aatagcattg gagcatggtt tagtatgtta
     1981 agctttgaaa aagagaactt aagagagtgt atttttgtat ctccaattct agatatgcaa
     2041 cagctcattt caaatatgat gctttgggca aatgtatcag aagaacagct taaacatgaa
     2101 ctaattatcc caacttcatt tgggcatact ctttcatggg aatatctaca atatgtaaaa
     2161 caacatccta tcactcaatg gaatgtacca acaaaaatat tatatggtaa acatgatgaa
     2221 cttactgaaa tcggtgttgt agagaaattt gtagaaaagt ttaattgcaa ccttactatt
     2281 atagaaaatg gcaaacattg gtttaacaca ccacaagaat taaattcatt atatgaatgg
     2341 ttaaatactt tagttaaata aaaaataatt atattattaa tatgattaaa ttatttataa
     2401 atgttattta attaatttta ttttagcatt attaaaataa ctacat
//
LOCUS       DAYAPA010000235         2394 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.240, whole genome shotgun sequence.
ACCESSION   DAYAPA010000235 DAYAPA010000000
VERSION     DAYAPA010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2394
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.240"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            239..>2394
                     /locus_tag="ACGI59_003774"
     CDS             239..>2394
                     /locus_tag="ACGI59_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HFG9741520.1"
                     /translation="MKIPKKIAAMLTVTMIAGSSTAGIASAQTVATNLTGQERYETAV
                     KISQDGWKNADEVVIVNDSSIADALSATPFAKAKNAPILLTSKDKLNDKTKAEIQRLK
                     AKKVYLIGGTSVLSTNIEKEIKDLKISFERISGAERYQTSLELAKKLDAISDVKKIAV
                     VNGEKGLADAVSVGAPAAQNNMPVILADSKNGTAVADKFIKDAGITQSYVVGGESSIS
                     EAVKNKLPNSTRLGGTDRNDTNAKVIKEFYKKTDLKNAYVTKDGMNKQDQLIDALAVG
                     VLGAKNQSPVVLVGKNLSASQKSLVNSKSFDKITKVGGNGNETAFNEMKSLQEVKTVE
                     AKTISELKSAIDKATANDVINFKPTSEVKEAFTIQTDKAVTVNLNGTYTKTVTINMPN
                     GDVNNYAKVDDVVIDDVKDGTFVNYGKITNLKVNDKNGAKIENNSKGEIGSLTVASGA
                     SQVKVTNGGKITTVTNNSKGTTIDNKGTISSVKGDNSPTISGNSPSSNSSGGSSSSGG
                     SSHGGGSSSGGSSSNQTSVNNEAAKITSVPTPAKDATRLTMPSVSSGYTIAIKTSSNE
                     SVIKKDGTIIPPNTATTVKLVFTVTHTSSGKTADTKELSVTVPAKSTDEELQAALDNE
                     VAKITSVPAPAKDATKLTMPSVSSGYTIAIKTSSNKSVIKEDGTIIPPNTEETVTLVF
                     TVTQESSGKTADTGEIDVVVPAKSTDGEIQAEVQAEA"
BASE COUNT         1041 a          321 c          435 g          597 t
ORIGIN      
        1 ttaacataaa aatcaaactt accttaaaat ataaatttac aaatttttca atcaataaga
       61 aaatttcttt attccttaca taatgactgc aatgtaacaa aataattaca aattaattat
      121 gaaatcatta tagttagaaa cctctttaaa attagatatt aagtatcctt tagaattaga
      181 acgggaacaa agttaaaaaa ttaagtaata tcaaatggac aagggggaag aaaataaaat
      241 gaaaatacca aagaagattg ctgcaatgct tacagttaca atgatagctg gaagttcaac
      301 agcaggcata gctagtgcac aaactgtggc aacaaattta acagggcaag aaagatatga
      361 aactgctgtt aaaataagtc aagatggatg gaaaaatgct gatgaggtag taatcgtaaa
      421 cgactcatct atagcagatg ctctatctgc aacaccattt gcaaaagcaa aaaatgctcc
      481 aatactttta acaagtaaag ataaattaaa tgataagacg aaagcagaaa tacaaagatt
      541 aaaagctaaa aaagtatact taataggtgg aacttctgta ttaagtacaa atatagaaaa
      601 agaaataaaa gatttaaaaa tatcttttga gagaatctct ggagctgaga gatatcaaac
      661 atccttagaa ttagcaaaga aattagatgc tataagtgat gttaagaaaa tagctgtagt
      721 taatggtgaa aaaggacttg cagatgctgt aagtgtagga gcaccagctg cacaaaacaa
      781 tatgccagta atacttgctg attctaaaaa tggaacagca gttgcagaca aatttataaa
      841 ggatgctgga ataactcaat cttatgtagt aggtggagag agttcaattt ctgaagctgt
      901 taaaaataaa ctaccaaata gtacaagact aggtggaaca gatagaaatg atacaaatgc
      961 aaaagtaata aaagaatttt ataaaaagac agatttaaaa aatgcttatg taactaaaga
     1021 tggtatgaat aaacaagacc aacttataga tgcactagca gtaggtgtat taggtgctaa
     1081 aaatcaatca ccagtagttt tagttggaaa gaacttatca gcttcacaaa aatctctagt
     1141 taactctaag agttttgata agataactaa agttggtggg aatggtaatg agacagcatt
     1201 taatgaaatg aagtcattac aagaagtaaa aacagttgaa gctaaaacaa tttctgaatt
     1261 aaaatcagct atagataaag ctactgctaa tgatgttata aactttaagc caactagtga
     1321 ggttaaagaa gcatttacaa tacaaactga taaagcagta acagtaaact taaatggtac
     1381 atatacaaag actgtgacta tcaacatgcc taatggtgat gtaaataact atgcaaaagt
     1441 ggatgatgta gttatagatg atgtaaaaga tggaactttt gttaattatg gaaaaataac
     1501 taacttaaag gttaatgata aaaatggagc caagatagaa aataattcta aaggtgagat
     1561 tggtagttta acagtagcat ctggagcaag tcaagtaaaa gtgactaatg gtggtaagat
     1621 aactacagtt acaaataatt ctaaagggac tactatagat aataaaggta caataagtag
     1681 tgttaaggga gataattctc caacaataag tggaaatagt ccaagttcta actcttcagg
     1741 aggaagttca tcaagtggag gttcttcaca tggaggaggc tcatcttcag gaggaagttc
     1801 ttctaatcaa acatcagtaa ataatgaagc agcaaaaata acaagcgtgc ctacaccagc
     1861 aaaagatgca actagattaa ctatgccatc agtatcaagt ggatatacta tagcaataaa
     1921 aacatcaagt aatgagagtg ttataaaaaa agatggaaca ataatacctc caaatacagc
     1981 aacaacagta aaattagtat ttacggtaac acatacatca agtggtaaaa cagcagatac
     2041 aaaagaatta tctgtaacag taccagctaa atcaacagat gaagaattac aagcagcatt
     2101 agacaatgaa gtggcaaaaa taacaagcgt tcctgcacca gcaaaagatg caactaaatt
     2161 aactatgcca tcagtatcaa gtggatatac tatagcaata aaaacatcaa gcaataagag
     2221 tgttataaag gaagatggaa caataatacc tccaaataca gaggaaacag taacattggt
     2281 atttacagta acacaagaat caagtggtaa aacagcagat acaggagaaa tagatgtagt
     2341 agtaccagcc aaatcaacag atggggaaat acaagcagaa gtacaagctg aggc
//
LOCUS       DAYAPA010000236         2386 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.241, whole genome shotgun sequence.
ACCESSION   DAYAPA010000236 DAYAPA010000000
VERSION     DAYAPA010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2386
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.241"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            177..2249
                     /gene="ptsG"
                     /locus_tag="ACGI59_003775"
     CDS             177..2249
                     /gene="ptsG"
                     /locus_tag="ACGI59_003775"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005355 - glucose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1904659 - glucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-specific PTS transporter subunit IIBC"
                     /protein_id="HFG9741521.1"
                     /translation="MKKVFGVLQKVGKSLMLPVALLPAAGILLGVSNALAGDALLAHF
                     PALANPTFQLIISIMESSGQIIFNNLPLIFAVGVAVGLTEGEGVAALAAIVGFLILNT
                     SMGVMAGVTEDMVDNSMYANIVGIPTIQTGVFGGIIIGIVAAYLYKKFYKIELPQYLG
                     FFAGKRFVPIVTSLVAIAIGIILSFIWPPIQEGLLSFSKNVIEANQTLAAFMEGSLER
                     LLIPFGLHHVFHNPFWYQFGEYVNKAGELVMGDQKIFFAQLKDGVNFTAGTFMTGAFP
                     FMMFGLPAAALAMVHEAKPEKKKFVAGIMASAALTSFLTGITEPIEFAFLFVAPVLFA
                     IHCLFSGLSYAIMQLLGVKIGFTFSGGLIDFLLFGVLPNRTPWYLAILVGIGFAVLYY
                     SVFRFMIRKFNLKTPGREDESETNAEVIEMSKNELASEILIALGNKDNLKSLDACITR
                     LRLVVNDINKVDKEKLKSLGAAGVMVIGNNVQAIFGPKSDSIKSDIKDIIEGKTKVES
                     TVTKNVDKNKVKKNMEVANMENTLVEKIDSLTNGELLDIEEVPDSVFSSKLMGDGFAI
                     KSSDGLIYSPVDGTIGVIFPTKHAIIIKSQKSGVEILIHLGIETVNLEGNGFEVFVNV
                     GDEVKAGEKLVKMDLEYIEKNGLSTISPVVFTNLEQNQKLSIKKGVTTAKEENRVSIL
                     RDDIEKIG"
BASE COUNT          880 a          255 c          450 g          801 t
ORIGIN      
        1 acttatgttt ataaaaaaat tataatagtt tatttttaat tctgaaatta ggttaatata
       61 ggtttgttta tattaacata agtttaattt tagaattaag tatactaatg taacttttta
      121 taaacttaag tcttttttat ttaaaaaaat ataaaataat aaggagagaa aagtaaatga
      181 aaaaagtatt tggtgtttta caaaaagtag gaaaatctct aatgctacca gttgcattac
      241 ttcctgcagc aggtatatta cttggcgtga gtaatgcttt agcaggagac gcattgttgg
      301 ctcattttcc agctttggcg aatcctacat ttcaacttat aataagtata atggaaagtt
      361 caggtcaaat tatatttaat aacttaccac ttatatttgc agttggggta gctgttggtt
      421 taactgaagg agaaggtgtt gcagcattgg cagccatagt aggtttttta attctcaata
      481 catctatggg tgttatggca ggtgttacag aagatatggt agacaattct atgtatgcaa
      541 atattgttgg aataccaaca attcaaacag gggtatttgg tggtattatt atagggatag
      601 tggctgctta tctttataaa aagttttata aaattgaatt acctcagtat ctaggttttt
      661 ttgcaggtaa aagatttgta ccaatcgtta cttcattagt tgctatagca ataggaatta
      721 tattgtcttt tatttggcct ccaatacaag agggattact tagtttctca aaaaatgtta
      781 tagaagcaaa tcaaacacta gcagcattta tggaaggttc tttggaaaga ctattaatac
      841 catttggttt gcatcatgtt ttccataatc cattttggta tcaatttgga gaatatgtaa
      901 ataaagctgg tgagcttgta atgggtgacc aaaagatatt ctttgctcaa cttaaagatg
      961 gagtaaattt cacagcaggt acttttatga caggtgcatt tccatttatg atgtttggtc
     1021 ttccagcagc agctcttgct atggtgcatg aagcaaagcc agaaaagaaa aaatttgtag
     1081 caggtataat ggcatcagca gcattaacct cttttttaac aggaataacc gagccaatag
     1141 agtttgcgtt tttatttgtt gcaccagtat tatttgcaat acattgttta ttttcaggtt
     1201 tatcatacgc aattatgcaa ttattaggag ttaaaattgg attcacattt tcaggaggat
     1261 taatagattt cttattattt ggtgttcttc ctaatagaac tccatggtac ttggctatct
     1321 tggtaggtat tggttttgca gtattatact atagtgtatt tagatttatg ataagaaaat
     1381 ttaatttaaa gacaccagga agagaagatg agtcagaaac taacgctgaa gtcattgaaa
     1441 tgtctaagaa tgaattagca tcagagattt tgattgcttt aggaaataaa gacaacttaa
     1501 agagtctaga tgcgtgtata acaagactta gattggttgt aaatgatata aataaagtag
     1561 ataaagaaaa attgaaatca cttggagcag caggtgttat ggttattggc aacaatgttc
     1621 aagctatatt tggaccaaaa tcagattcaa taaagtctga tataaaagat ataatagagg
     1681 gaaaaacaaa ggttgaaagt actgttacta aaaatgttga taaaaataaa gtgaaaaaaa
     1741 atatggaagt tgcaaatatg gaaaatactt tagtagaaaa gattgattca ttaacaaatg
     1801 gagagttatt agatatagaa gaagtgccag attctgtatt ttcaagtaaa ctgatggggg
     1861 atggatttgc aattaaatct agtgatggat tgatttattc accagttgat ggaactattg
     1921 gagttatatt tccaactaag catgcaataa taataaaatc ccaaaaaagt ggagtagaaa
     1981 tacttataca tttagggata gaaactgtaa acttagaagg aaatggattt gaagtatttg
     2041 tgaatgtagg agatgaagta aaagcgggag aaaaattagt taaaatggac ttagaatata
     2101 ttgaaaaaaa tggtctatca acaataagtc ctgtagtatt tacgaattta gaacaaaatc
     2161 agaaattaag cattaaaaaa ggagtaacca cagcaaaaga ggagaataga gtaagtatat
     2221 taagagatga tattgaaaaa ataggatagc tatatgatag tgtaactaaa agttatgtga
     2281 gatttaataa atatataaat aggttcagtt aacatgcaat ttatttaata tatattttaa
     2341 gtaatagaaa tatatatcat tatattttga tatattttaa aacatt
//
LOCUS       DAYAPA010000237         2338 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.242, whole genome shotgun sequence.
ACCESSION   DAYAPA010000237 DAYAPA010000000
VERSION     DAYAPA010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2338
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.242"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            <1..1088
                     /locus_tag="ACGI59_003776"
     CDS             <1..1088
                     /locus_tag="ACGI59_003776"
                     /inference="COORDINATES: protein motif:HMM:NF012403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HFG9741522.1"
                     /translation="DWVHHFKTYYPDMGIINGTKKLSEGIEYFKIVEVPFIVVINYEL
                     AFRRKELLDLKDFTLMLDESSMIQNEKAKRSKFVLSLNPKNVILLSGTPCSGKYEQLY
                     SQIKLLGWNISKDLYWKQYIDVEYKDIGGFPMKVVTGYKNVDRLKKKLRDYGAVFMMS
                     DEVFDLPKQIDNVMKISTTREYRKFKKDSIISISDDIELVGDTTLTKMLYERQLCSQY
                     NKFKLEAFGDLINSTSDRLIVFYNFNEELDRLIELSRDRPISIVNGSIKDLSNYEEYN
                     NSITFVQYQAGAMGLNLQKCNKIVYFSLTLSCELFMQSKKRVHRIGQENTCFYYYMIC
                     RNSVEENIYKSLQSGVDYTNNLFERGE"
     gene            1092..1394
                     /locus_tag="ACGI59_003777"
     CDS             1092..1394
                     /locus_tag="ACGI59_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003500708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HFG9741523.1"
                     /translation="MGAEKQFENKVKKFLKEQGCWFIKYWGGSAYTKSGIPDLLVCCS
                     GKFIGIEVKGEKGKASELQKYNIKEIEKAGGIGIILYPKDFDRFKKMILEIKKEGE"
     gene            1395..>2338
                     /locus_tag="ACGI59_003778"
     CDS             1395..>2338
                     /locus_tag="ACGI59_003778"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HFG9741524.1"
                     /translation="MQFSHSRIGVFKSCPYKYKLQYIDKVKTILNADANNALILGTSL
                     HTGIEKDIDAAVKEYYFSYPSITDLHINEVMKLDYLIPKVKEILPKGFNEVQITTSDF
                     IGFIDLLAPHVYMTDTNLNTNETTIYYEDEERVFDIYDFKYSNNIDRYLESEQLHLYK
                     YFYEKQHTNHEIKDLYYVFVPKIQIRQKKTENLHQFRQRLLNELDKSEIKIVKVEYDP
                     NKVINFLVDIKRCLENKEFEKNQNKLCNWCDYQEYCEKGIDYMLLPNSERRSVEGINK
                     KTLWIYGAPFSGKTTLANDFEKPLMLNTDGNIKFVDAP"
BASE COUNT          972 a          221 c          421 g          724 t
ORIGIN      
        1 acgattgggt acatcacttt aaaacatatt atccagatat ggggataatc aatggtacaa
       61 agaaacttag tgaaggcata gagtatttta agattgttga agttccattt atagtagtaa
      121 ttaattatga attagctttt agaagaaaag agttattaga tttaaaagat tttactttaa
      181 tgttagatga atcatcaatg attcaaaatg aaaaagctaa aagaagtaag tttgtactaa
      241 gtttaaaccc taagaatgtg atactacttt ctggtactcc atgtagtggt aaatatgagc
      301 aactatattc tcaaattaaa ctactagggt ggaatatatc aaaagattta tattggaagc
      361 aatatattga tgttgagtac aaagatattg gtggttttcc aatgaaggtt gtaacaggtt
      421 ataagaatgt tgacagatta aaaaagaagt taagagatta tggagcagta tttatgatgt
      481 cagatgaagt atttgactta ccaaaacaga tagataatgt aatgaaaatt agtactacaa
      541 gagagtatag gaagtttaaa aaagattcaa ttataagtat ttcagatgat attgaacttg
      601 tgggagatac tacactaact aaaatgttat atgaaagaca attatgtagt caatacaata
      661 aatttaaatt agaagctttt ggagacttaa taaactcaac aagtgacaga ttaatagtat
      721 tttacaattt taacgaagag ttagacagat taatagaatt atcaagagat agaccaatat
      781 caatagtaaa tggttctata aaagatttaa gcaattatga ggaatataat aactcaatta
      841 catttgtaca atatcaagca ggagctatgg gtttaaatct tcaaaagtgt aataagattg
      901 tatactttag tcttacttta tcttgtgaat tgtttatgca aagtaaaaag agagttcata
      961 gaataggtca agaaaacaca tgtttttatt attatatgat ttgtaggaat agtgttgaag
     1021 aaaatatata taaaagctta caaagtggtg ttgactatac aaataatttg tttgagagag
     1081 gagagtaata cttgggtgca gaaaaacaat ttgaaaataa agttaaaaag tttttaaaag
     1141 aacaaggttg ttggtttatt aaatattggg gaggttcagc atatacaaaa agtggtatac
     1201 cagatttatt agtttgttgt agtggtaaat ttataggtat tgaagtcaag ggggaaaaag
     1261 gaaaagcaag cgaactacaa aagtataata ttaaagaaat tgaaaaggca ggtggaatag
     1321 gaattattct ataccctaaa gactttgata gatttaaaaa gatgatttta gaaataaaga
     1381 aagaaggtga gtaaatgcag ttctcacata gtaggatagg agtatttaag agttgcccat
     1441 ataagtataa gctacagtat attgacaagg tgaaaacaat attaaatgca gatgctaaca
     1501 atgctctaat attgggaaca tcacttcata caggtattga aaaagatatt gatgcagcag
     1561 taaaagaata ttattttagt tatccaagta taacagattt acacattaat gaagtgatga
     1621 agttagatta tttaatacca aaagtaaaag aaattttacc aaaagggttt aatgaggttc
     1681 aaataacaac tagtgatttt atagggttta tagatttgtt agcaccacat gtatacatga
     1741 ctgatacaaa cttaaataca aatgaaacaa ccatatatta tgaagatgaa gaaagagtat
     1801 ttgatattta tgactttaaa tattcaaaca acatagatag atatctagaa agtgagcaac
     1861 ttcatctata taagtatttt tatgaaaagc aacatacaaa tcatgagata aaagatttgt
     1921 actatgtatt tgtacctaaa attcagatta gacagaagaa aacagaaaac ttacatcaat
     1981 ttagacaaag attacttaat gagcttgata aatctgaaat aaaaatagtg aaggttgagt
     2041 atgacccaaa taaagttatt aatttccttg tagatataaa aagatgtctt gaaaacaagg
     2101 aatttgaaaa aaatcaaaat aaattatgta actggtgtga ctaccaagaa tactgtgaaa
     2161 aggggattga ttatatgtta ttaccaaata gtgaaagaag aagtgtggaa ggaattaata
     2221 aaaaaacttt atggatttat ggagcaccat ttagtggcaa aaccacatta gcaaatgatt
     2281 ttgaaaagcc tttaatgctt aatacagatg gaaatataaa gtttgttgat gctccata
//
LOCUS       DAYAPA010000238         2322 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.243, whole genome shotgun sequence.
ACCESSION   DAYAPA010000238 DAYAPA010000000
VERSION     DAYAPA010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2322
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.243"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            198..2210
                     /locus_tag="ACGI59_003779"
     CDS             198..2210
                     /locus_tag="ACGI59_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFG9741525.1"
                     /translation="MKKSVALVNDSRKDLIDFLENNLKLVFGDSININRYFINEINDN
                     DIINDDVILVMSVERLDKIINNILDKKKVIVVRRTFREDKIYNLLSLPQGTNVLIVND
                     SDETTLETISLFYKIGVTNIRPIPYMNDNNYKNIKIAITPGVPEKVPSFISDIFDLGH
                     RYIDISTFIEIINLLQIDSKEIQSNLVKYSEEIISLDTGIKDKYKELFLKIEELDTIL
                     NLSKDGILFTSKDGEINTYNSKVKDILDINEDIYGKYIEDIFVDSLKVLLSEKEILDK
                     VVVFNKKYINVNKKNIYNRDEKMGTYYSLQEITYIKKLEQNLTKKLREKGQIAKYTFK
                     DIKTNSPKMFECIDLAKKVSKSDLSILIRGESGTGKELIAQSIHNNSNRKNQPFIAVN
                     CAAVPENLLESQLFGYDKGTFTGGLKDGKQGLFELANNGTIFLDEIGDMPLELQTKLL
                     RVLQEKQIMPVGSHNVINIDVRIISATNKNLEQMIDNSQFREDLYYRLNTIPINIPPL
                     RERKEDILIIMEDLINKKLVITPEAKKLIQNYMWKGNIRELQNVTSYLNIMCEDIVLE
                     KDLPPNLRSSDNKNTSLKLKYSKNDILNILEILILNKESDVGIGRGLILKALLDKNLQ
                     ITEGKIKKIFEYLKKEELIICSSGRYGSKITQKGEDFYNKLKYKGL"
BASE COUNT         1032 a          225 c          334 g          731 t
ORIGIN      
        1 acagtttaag ttttaagcaa caattagggg gatttggtga caagattttt aattataaga
       61 aagaaagtgt cttaatgcaa cttttataaa gtcattatag acactttctt ttttatttga
      121 taaatttaaa ttatcaaaat tatattgctt tatacaacta ttttgttgga aactatttat
      181 tatataagga gaaataaatg aaaaaatcag tagctctggt aaatgatagt agaaaagatt
      241 taatagactt tttagaaaat aatttgaaat tagtttttgg agattctata aatattaata
      301 gatacttcat aaatgaaata aatgataatg atattataaa tgatgatgtg atattagtaa
      361 tgtcagtaga aagattagac aaaattataa ataatatact agataagaaa aaagtgattg
      421 tggtaagaag aacttttagg gaagataaaa tttataatct actatcttta ccacagggta
      481 caaatgttct aatcgtaaat gattctgatg aaactactct agaaactata agtttatttt
      541 ataaaattgg agtgactaat attagaccaa ttccatatat gaatgataat aactataaaa
      601 atataaaaat agctataact ccaggagttc cagaaaaagt acctagcttt atctcagata
      661 tatttgattt aggtcacaga tatatagata tttctacatt tatagaaata ataaatttat
      721 tacaaattga ttcaaaggaa atacaatcta atctagttaa atactcagaa gaaataataa
      781 gtttagatac tggtataaaa gataaatata aggaattatt tttaaaaata gaagaattag
      841 atacaatatt gaatttatca aaagatggta tattatttac ttcaaaagat ggtgaaataa
      901 atacatataa tagtaaagta aaagatattt tagacataaa tgaggatata tatggaaaat
      961 acattgagga tatatttgta gatagtttaa aagttttatt gagtgagaaa gagatactag
     1021 ataaagttgt cgtattcaat aagaaatata taaatgtaaa taaaaaaaac atatacaata
     1081 gagatgaaaa aatgggcaca tattatagtt tacaagaaat aacatacata aaaaaattag
     1141 aacaaaatct cactaaaaaa ttaagagaaa aaggacaaat agcaaaatat acatttaagg
     1201 atataaaaac aaatagtcct aaaatgtttg aatgtataga tttagctaag aaggtttcta
     1261 aatctgactt aagcatactt atcagaggtg aaagtggaac tggtaaagaa ctcatagctc
     1321 agtctataca taacaactca aatagaaaaa atcaaccctt tatagcagta aattgtgcag
     1381 ccgttcctga aaatttatta gaaagccagc tattcggata tgacaaggga acttttactg
     1441 gaggtctaaa agatggaaaa caaggattat ttgaacttgc taataatgga acaatattct
     1501 tagatgaaat aggcgatatg cctttagaat tacaaacaaa actacttaga gttcttcaag
     1561 aaaagcagat aatgcctgtt gggtctcaca atgtaattaa tatagatgtt agaatcatat
     1621 ctgctacaaa taagaattta gaacaaatga ttgataatag ccaatttaga gaggatttat
     1681 attatcgatt aaatacaatt ccaataaata tacctcctct aagagaaaga aaagaagaca
     1741 tattaattat tatggaagat ttaattaata agaaattagt aatcacacca gaagcaaaaa
     1801 aacttataca aaactatatg tggaaaggta atataagaga actccaaaat gttacatcct
     1861 atttaaacat tatgtgtgaa gatattgtat tagagaaaga tttacctcca aacttaaggt
     1921 cttcagataa caaaaataca tcattaaaac taaaatatag taagaatgat atattgaata
     1981 tattagagat actaatttta aataaggaat cagatgttgg tattggtaga ggattaattt
     2041 taaaagcact actcgataag aaccttcaaa ttacagaagg aaaaattaaa aaaatatttg
     2101 aatatctaaa aaaagaagaa cttataattt gtagttcagg aagatatgga agtaaaataa
     2161 ctcaaaaagg agaagatttt tacaataaat taaaatataa aggtctataa aattataatt
     2221 tatcaaacaa agaaatctat tcaggattaa agtactcctt caataggttt ttttattttt
     2281 caagtaccct gaaattataa tttattttgt acataaataa ta
//
LOCUS       DAYAPA010000239         2295 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.244, whole genome shotgun sequence.
ACCESSION   DAYAPA010000239 DAYAPA010000000
VERSION     DAYAPA010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.244"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(63..263)
                     /locus_tag="ACGI59_003780"
     CDS             complement(63..263)
                     /locus_tag="ACGI59_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFG9741526.1"
                     /translation="MKNGIVKWFNNEKGFGFISVEGEDDVFVHFSAIQNDGYKTLEEG
                     EKVSFDITQGNRGPQAENVNRI"
     gene            730..2220
                     /locus_tag="ACGI59_003781"
     CDS             730..2220
                     /locus_tag="ACGI59_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFG9741527.1"
                     /translation="MFQFETQIHKLRHDVLTAIARLGKEDNLTREAMDNIQYEIIKGE
                     KPTYRCCVYKERAIISERVNVSMGLQPGQDKIDLDTMDIEEDEQIIYILEAACDSCPI
                     NEFTVTDICRGCLAHRCKEACKFGAITHVGGMAYINHELCKACGMCKKACQYDAISEV
                     VRPCKSVCPTNALGFDRENMKAMIHEDKCLNCGACMSACPFGAISDKSLIAPVARKLV
                     QKEKMYAVVAPAITGQVEANVTYGQIKNAIKSLGFVDVLEAACGADAVTVHESLEFAE
                     RMEKGEAFMTNSCCPGFVGYIEKTYPNHVGKISSTVSPMIASGRFIKSHEPDAKVVFI
                     GPCTAKKSEAAKKELKGAVDYVLTFEEIMALFEAFDIDLAKCSNEDIDDASIFGRGFG
                     AAGGLTKAIENYLAEKGIGVNFEPVKISGSREIKKTMTMANLGKLEGNFIEGMMCEGG
                     CINGSGKFLSGAKVKATFDRKNSQSTKKSVLANTKIKDFSNINLEK"
BASE COUNT          918 a          255 c          404 g          718 t
ORIGIN      
        1 aaacaacaaa aaagagccct cgatagctcc ttttctaaaa tatatatatt taaattaaat
       61 aattatattc tatttacgtt ttcagcttga ggtcctctat taccttgagt tatatcaaag
      121 ctaacctttt cgccttcttc taaagttttg tatccatcat tttgtatagc tgagaaatgt
      181 acgaaaacat catcttctcc ctctacagat atgaacccaa atcctttttc attgttaaac
      241 cattttacta ttccgttttt cattataaga aaacctccgc attattaaaa aatacaaact
      301 aaaaaaataa gtctgttcta ttaatataac ataaatttta aataaaagca aataaaattt
      361 ttaaaaaaat taattacttt tagaatttaa taaaaattgt taaattatac agtgagaagt
      421 attgtaaaat ttgacgaaaa tgtaaatgta gataataaat ttaatatcaa aaaatgtaac
      481 aaaaaggtat gtaagtaaaa agtaaaatta aaatatataa taaaaaatat tatatattag
      541 attgaaaagt aaaaataaaa atagtgaaat ttcagaaata tcaataatat tatataataa
      601 aacaaaaaaa cactatatat attcgataaa ataaaattta aacatataat attttatgta
      661 tatattaata aaaggacata ttgtgttata atggtatcgt ataaaatcat ttacgggggt
      721 ggtattaaaa tgtttcaatt tgaaacacaa atacacaaat taagacatga tgttttaact
      781 gcaatagcta gacttggaaa agaagataac cttaccagag aagcaatgga taatattcag
      841 tatgaaataa taaaaggtga gaaacctaca tacagatgtt gtgtttataa agaaagagct
      901 ataatatctg agagagtaaa tgtaagtatg gggttacaac caggtcaaga taagatagat
      961 ttagatacaa tggatataga agaagatgaa caaattatat atattttgga agctgcatgt
     1021 gacagttgtc caataaatga atttacagta acagatattt gtagaggatg tctagcacat
     1081 agatgtaaag aagcttgtaa atttggagct ataacacatg tcggaggaat ggcctatata
     1141 aatcatgaat tatgtaaggc ttgtggaatg tgtaaaaaag cttgtcaata tgatgccatc
     1201 tcagaggttg ttagaccatg taagagtgtt tgtccaacta atgccttagg atttgataga
     1261 gaaaatatga aagctatgat acatgaggat aaatgtttaa actgtggagc atgtatgtca
     1321 gcatgtccat ttggagcaat atctgataag agtttgatag ctcctgtagc tagaaagtta
     1381 gttcaaaaag agaaaatgta tgcggtggta gctccagcta taacaggtca agttgaagca
     1441 aatgttacat atggtcaaat aaaaaatgct ataaaatcac taggatttgt tgatgtatta
     1501 gaagcagctt gtggagcaga tgcagttaca gttcatgaaa gcttagaatt tgctgaaaga
     1561 atggaaaagg gagaagcttt tatgacaaat tcttgttgtc caggatttgt tggttatata
     1621 gaaaaaacat acccaaatca tgtagggaaa atatcttcta cagtatctcc tatgatagct
     1681 agtggtagat ttataaaaag tcatgaacca gatgctaaag ttgtattcat aggaccatgt
     1741 acagccaaaa agagtgaggc agctaagaaa gaattaaaag gagctgttga ttatgtactt
     1801 acttttgaag aaataatggc attatttgag gcatttgata tagacttggc taagtgttca
     1861 aatgaagata tagatgatgc atcaattttt ggaagaggtt ttggagctgc tggtggtctt
     1921 acaaaagcta tagaaaatta tcttgctgaa aaaggtattg gtgttaattt tgaacctgta
     1981 aagatttctg gttcaagaga aatcaagaaa actatgacta tggctaattt aggaaagcta
     2041 gaaggtaatt ttatagaagg aatgatgtgt gaaggtggtt gtataaatgg ttctggtaaa
     2101 ttcttatcag gtgcaaaggt taaagccact tttgatagaa aaaattctca atcaactaag
     2161 aaaagtgtct tagcaaatac aaagataaaa gattttagta atataaattt agaaaaataa
     2221 agataataca aagcaacaaa aatacatatt gttaatagag ttgttctaag ttgaggttta
     2281 acttttggac aactc
//
LOCUS       DAYAPA010000240         2290 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.245, whole genome shotgun sequence.
ACCESSION   DAYAPA010000240 DAYAPA010000000
VERSION     DAYAPA010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2290
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.245"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(130..1422)
                     /gene="brnQ"
                     /locus_tag="ACGI59_003782"
     CDS             complement(130..1422)
                     /gene="brnQ"
                     /locus_tag="ACGI59_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFG9741528.1"
                     /translation="MGEKQINFKDVIVIGFALFAMFFGAGNLIFPPYLGVLSGSSWLV
                     AFIGFLFADGGLALLAVIAATKFNGDTSKMFSRAGKGLSIALGCAMVICIGPLLAIPR
                     TAATTYEMGILPTIGSGISPVIFSIVFFALVLVLTIRPSKVVDIVGSILTPALLIALA
                     VLIVKGIVSPLGEIRDSSLIQNVFAEGITQGYQTMDALAASVFASIIIMSVIAKGYTG
                     EKEKMKATISAGVIAVIGMALVYGGLCYLGATVSQQYGQDVQQTALIVAITAALLGTT
                     GKILLAIIVALACLTTAIGLSSAAGQYFSTLTDGKLKYEHIVIVVCVFSAIISNFGVS
                     TIIQFSSPILSMVYPATITLVILALFSNKIKNDNVFKCAAYMALLVSVLTVATSFGVK
                     IPLVNSLPFASLGFNWIVPVVIAGIIGNFIPSKSSKTA"
BASE COUNT          762 a          377 c          249 g          902 t
ORIGIN      
        1 ttttaaattt atcaaacagg attaataatg ttcttttatc tacaataatt taataattct
       61 tttaaatata aaaaggaatt gcctatataa tttaaaggca attccataat tatcaattag
      121 tttttattat tatgcagttt tactgctttt agatggaata aagtttccta ttattcctgc
      181 tattacaact ggaactatcc agttaaatcc taatgaagca aatggtaagc tattaactag
      241 aggaattttt acaccaaaac ttgttgcaac tgttaataca cttactaata atgccatgta
      301 tgcagcacat ttgaacacgt tgtcattttt aattttatta ctaaataatg ctagtataac
      361 tagagttata gttgctggat ataccatact tagtatagga gaagagaatt gtattattgt
      421 acttactcca aagtttgata tgattgcact aaatacacaa actactataa ctatatgctc
      481 atactttaat tttccatctg taagtgttga gaaatattga ccagctgctg aagaaagacc
      541 tatagctgtt gttaaacatg ctaatgcaac tataatagcc aataaaattt tacctgtagt
      601 tccaagtaaa gctgctgtta ttgcaactat taaagctgtt tgttgaacat cttgtccata
      661 ttgttgagac acagttgctc caagataaca taatccacca taaactaatg ccattcctat
      721 tactgcaatt acacctgcac ttattgtggc tttcatcttt tctttctcac ctgtataacc
      781 tttagctatt actgacatga ttataattga tgcaaataca ctagctgcca atgcatccat
      841 tgtttgataa ccttgagtta taccttctgc aaatacattt tgtattaaag aactgtctct
      901 tatttctcct aatggagata cgataccttt aactattaat actgctaaag ctataagtaa
      961 agctggagtt aatattgacc caactatatc tacaactttt gatggtctta ttgttaaaac
     1021 caatactaga gcaaagaata ctatagagaa tattactggg cttattccag aacctattgt
     1081 aggtaatata cccatttcat aagttgttgc tgctgttcta ggtattgcta aaagtggccc
     1141 tatacagatt accattgcac atcctaatgc aatacttaat cctttgcctg cccttgagaa
     1201 catcttcgat gtgtctccat tgaacttagt tgctgctata actgctaata atgccaaacc
     1261 accatctgca aaaaggaatc ctatgaaagc tactagccaa ctacttccag ataaaactcc
     1321 tagatatgga gggaagatta agttccctgc accaaaaaac attgcaaata gtgcaaatcc
     1381 aattacaatt acatctttaa aattgatttg tttctcaccc atttaaagca cccccatttg
     1441 aattttacta ccttttacta aaaaataagt ccaatctctt aattaaatga aaaccttttg
     1501 acgagatttt atttaattat acatttttga cttaaatttg tcaattatgt ttcataattt
     1561 ataaaatctt cacaatattt aatacaactt tcttaattaa acaaaatcaa cctgtatact
     1621 ttaaacatag ttggtttttt gttttttctc taatttttct tgataaaatt aactattccg
     1681 aataattaaa atattaagac tttttagatt attcgattta ttattaagtt gtattgcttt
     1741 atatactgta tatatcactc tcctttttta cattcatata atctttttaa ttcaatttaa
     1801 attttaaata ttttgtgttt attatataat ctgaaaaaaa agcagtcaat agcattttta
     1861 atttttttaa ttttcaaaat tatgattctt aaaaaatcgt tttcctttta tcataacacc
     1921 tatttacgtt aattcttgta gtattttgta tttattttgc taaattgaca gttaacttta
     1981 ttcaattttt attctacttg cgtagatttg cctcttgttt tttactctta aaaccatttt
     2041 ttctgaaaat tccttattat gatttatcaa cttatttttc ttcataacaa ttactctcta
     2101 acttccaact tacttaaact ttgcagttgc tagtattcac gtttacatac ttatatgtca
     2161 taattaacta aaaattctta atatatctct caatgaaaac aatttactca tttatttaat
     2221 acatttatat aatcatatgt cttaaatttt gttttattat tttattattg tcacaatttt
     2281 atttatatta
//
LOCUS       DAYAPA010000241         2269 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.246, whole genome shotgun sequence.
ACCESSION   DAYAPA010000241 DAYAPA010000000
VERSION     DAYAPA010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2269
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.246"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            124..264
                     /locus_tag="ACGI59_003783"
     CDS             124..264
                     /locus_tag="ACGI59_003783"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9741529.1"
                     /translation="MEKSNTTVTKKEKIEDIGMKLEHLLSEDITFYELLLEILYKEKE
                     EN"
     gene            complement(261..632)
                     /locus_tag="ACGI59_003784"
     CDS             complement(261..632)
                     /locus_tag="ACGI59_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFG9741530.1"
                     /translation="MESLGDRIANLRKELDINQKELATKVGITEASLSRYENNLREPK
                     SEIIVRLAKALETSTDYLLGVNDNTKISKEDKLIIENLSVSEKTKKLLEKIYSLEKED
                     REAIEKMIDNAYLKRFLKEEN"
     gene            1181..1438
                     /locus_tag="ACGI59_003785"
     CDS             1181..1438
                     /locus_tag="ACGI59_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9741531.1"
                     /translation="MIIIRSQDKTNLMQINQIKIDGSQVYAVFESKIDTVKIGDYENN
                     TRAIQVLDNIQNFIENGTKYDYITSNKVRYNVNKIFQMPAK"
     gene            complement(1548..2228)
                     /locus_tag="ACGI59_003786"
     CDS             complement(1548..2228)
                     /locus_tag="ACGI59_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFG9741532.1"
                     /translation="MIWTFYSFICVILPCLIYQIVVIKRKNLKVEKNVMIHLVWVYIF
                     LLYIYLALSKAGIGSIWDIGRYSGLFRIDEINLIPFDSVGILTYILNIIMFMPLGFLL
                     PLIWKNFRNIINVSLTGLGFSLAIELCQLFNLRATDIDDLMMNTLGAVLGYFIWVGVN
                     NLFNLIKKKDIFPSIYKNIIQDEIAITIDDDISKKITSLYKNEAIIYLILAVLGEFLL
                     FNWSIIPY"
BASE COUNT          916 a          255 c          260 g          838 t
ORIGIN      
        1 gaaattttgg ataatcaaaa tatattttat aaaatcaaaa tacaattaca taaactttat
       61 taaaaatttg ttgggaattt ctgactaaca ttaagaacgt aagtttgttg ggggatggta
      121 tatgtggaaa agtcaaacac tactgttaca aaaaaagaaa aaattgaaga tataggaatg
      181 aaattagaac atttattgag tgaagatatt actttttatg aattattact tgagattttg
      241 tataaagaaa aagaagagaa ttaattctct tctttaagga atctttttaa ataggcatta
      301 tcaatcattt tttctatagc ttctctatct tctttttcaa gagaatatat tttttctaag
      361 agcttttttg ttttttcact tactgataag ttctctatga ttaatttatc ttctttgctt
      421 atttttgtat tatcatttac gcctaataaa taatctgttg atgtttctaa tgcttttgct
      481 aatctaacta ttatttcact tttaggttct cttaaattat tttcataacg tgacaagcta
      541 gcctcagtta taccgacttt agtagcaagt tctttttgat ttatatctaa ttcttttcgt
      601 agatttgcta ttctatctcc taaactttcc atatgtacat cactccttac cgaaattata
      661 agttattttg ataaaaagta aaataaaatt accaaaaata taaaaaaaaa aggttaaaat
      721 aataaaatat attgaaaatt accatttgta tatgtataat atgaattata caaatggtaa
      781 tttgatgaga ggtggtatgt tgaaataaga aactgaatta attaataaat acatagaaag
      841 ttttgaaaac ctttaattga gatataaaca atatgtacaa aattttttgc aagtaaaatt
      901 accaaatgta taaaataaat cagtagcata gataatttta tgttttaata cattagatta
      961 tttaatttag ttataatggt caaaaatgca atctacaaat aaaacaaagt attatcaaaa
     1021 aagtattctt taagaaaatt ttttattaga gatgtaattg atataaataa tcgtattaaa
     1081 aaataaaata ctaagtttaa aaatttaaat aatctataaa tcaattataa aatattattt
     1141 attagaaatt aaaatgaatt aagtaaatgg aggaattgat atgataataa ttagaagtca
     1201 agataaaact aatttaatgc agataaacca aattaaaata gatggtagtc aagtttatgc
     1261 agtatttgaa agtaaaattg atactgtaaa aataggagat tatgaaaata atacaagagc
     1321 tatacaagtg ttggacaata tacaaaactt cattgaaaat ggaacaaaat atgattatat
     1381 aacatcaaat aaagtcagat acaatgttaa taaaatattt cagatgccag ctaaatagtg
     1441 agtgtgcatt ttaatttaat aactaaagcc cactttcttt tgtagtatga aagagggctt
     1501 tacatatttg taaatatcga agtacactta ttttatcaat aaattattta ataaggtata
     1561 atggaccaat taaacaataa aaattctcct aaaacagcaa gtattaaata tataatagct
     1621 tcatttttgt aaagagaggt aatttttttg cttatgtcat catcaattgt aatagcaatc
     1681 tcatcttgaa taatattttt atatatagat gggaaaatat cttttttctt tataagatta
     1741 aataaattat ttacacctac ccatataaaa tatcctaaaa cagcacctaa tgtgttcatc
     1801 attaaatcat caatatctgt agctcttaag ttaaacaatt gacaaagttc aattgctaat
     1861 gaaaaaccta atccagttaa tgaaacattg ataatatttc taaaattttt ccaaattaaa
     1921 ggtagtaaaa atcctaaagg cataaacata attatattta aaatatatgt taagatgcca
     1981 actgagtcaa aaggtattag atttatttca tcaattctaa aaagcccact ataccttcct
     2041 atatcccata tgctaccaat tccagcttta ctaagtgcta gatagatata taataaaaaa
     2101 atatataccc atactaaatg tatcattaca ttcttttcaa ctttcaaatt ttttcgtttt
     2161 ataacgacta tttggtatat taagcatggt aatataacgc aaataaatga ataaaatgtc
     2221 catatcattt tattttgtcc cctttcaaat ttatgttttt agttttata
//
LOCUS       DAYAPA010000242         2154 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.247, whole genome shotgun sequence.
ACCESSION   DAYAPA010000242 DAYAPA010000000
VERSION     DAYAPA010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2154
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.247"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            133..831
                     /gene="tcdC"
                     /locus_tag="ACGI59_003787"
     CDS             133..831
                     /gene="tcdC"
                     /locus_tag="ACGI59_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin anti-sigma factor TcdC"
                     /protein_id="HFG9741533.1"
                     /translation="MFSKKNEGNEFSNEGKGSSKKIIKFFKSTKGIALLAFILGVFFG
                     NISSPACSEDHEEVISNQTSVIDSQKTEIETLNSKLSDAEPWFKMKDDEKKAIEAENQ
                     RKAEEAKKAEEAKKAEEQRKKEEEEKKGYDTGITYDQLARTPDDYKYKKVKFEGKVIQ
                     VIEDGDEVQIRLAVSGNYDKVVLCSYKKSITPSRVLEDDYITIRGISAGTITYESTMG
                     GNITIPGIAVEKIN"
     gene            complement(1166..>2154)
                     /locus_tag="ACGI59_003788"
     CDS             complement(1166..>2154)
                     /locus_tag="ACGI59_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_105161403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase C80"
                     /protein_id="HFG9741534.1"
                     /translation="NTFIASTGYTSINGKHFYFNTDGIMQIGVFKGPNGFEYFAPANT
                     HNNNIEGQAILYQNKFLTLNGKKYYFGSDSKAVTGLRTIDGKKYYFNTNTAVAVTGWQ
                     TINGKKYYFNTNTSIASTGYTIISGKHFYFNTDGIMQIGVFKGPDGFEYFAPANTDAN
                     NIEGQAIRYQNRFLYLHDNIYYFGNNSKAATGWVTIDGNRYYFEPNTAMGANGYKTID
                     NKNFYFRNGLPQIGVFKGSNGFEYFAPANTDANNIEGQAIRYQNRFLHLLGKIYYFGN
                     NSKAVTGWQTINGKVYYFMPDTAMAAAGGLFEIDGVIYFFGVDGVKAPGIYG"
BASE COUNT          812 a          292 c          322 g          728 t
ORIGIN      
        1 ctttattttg taaaattatg cttaggggaa atatatttta ggaaaatatg aatatataat
       61 ttttagtcaa ctagttattt taagttttta acttttaaaa taaaatatat ctaataaaag
      121 ggagattgta ttatgttttc taaaaaaaat gagggtaacg aatttagtaa tgaaggaaaa
      181 ggaagctcta agaaaataat taaattcttt aagagcacaa agggtattgc tctactggca
      241 tttattttag gcgtgttttt tggcaatata tcctcaccag cttgttctga agaccatgag
      301 gaggtcattt ctaaccaaac atcagttata gattctcaaa aaacagaaat agaaacttta
      361 aatagcaaat tgtctgatgc tgaaccatgg ttcaaaatga aagacgacga aaagaaagct
      421 attgaagctg aaaatcaacg taaagctgaa gaagctaaaa aagctgaaga agctaaaaag
      481 gctgaagaac aacgcaaaaa agaagaagag gagaagaaag gatatgatac tggtattact
      541 tatgaccaat tagctagaac acctgatgat tataagtaca aaaaggtaaa atttgaaggt
      601 aaggttattc aagttattga agatggtgat gaggtgcaaa taagattagc tgtgtctgga
      661 aattatgata aggttgtact atgtagttat aaaaaatcaa taactccttc aagagtatta
      721 gaggatgatt acataactat aagaggtata agtgctggaa ctataactta tgaatcaact
      781 atgggtggaa atataactat accagggata gctgtagaga aaattaatta atattgtttt
      841 gtattatagt taatatttta tattatagtc aatatgttta aagatgtttt tataattgca
      901 aataaacagt tacaaggctc taaattagtt tttgctttta gcatattatc tattttttat
      961 caactattaa ttatttagta ttaatatttc catatatgaa ttttattata aaatagtcaa
     1021 gaataataga ttattaaatg atagaaaaat tttaactaaa agtcatgtat tacaataaca
     1081 catgactttt aattaaatct caatatttat tatataaaaa taatttctta gtagcacagg
     1141 aataattttt tatcaaacat atattttagc catatatccc aggggctttt actccatcaa
     1201 caccaaagaa atatataaca ccatcaatct cgaaaagtcc accagctgca gccatagcag
     1261 tatcaggcat aaagtaatat actttaccat taatagtttg ccatccagta actgcttttg
     1321 aattattacc aaagtaatat atttttccaa gtaaatgtag gaatctattt tgataacgta
     1381 tagcttgacc ttctatattg ttagcatccg tattagcagg tgcaaagtat tcaaatccat
     1441 tagacccttt aaacactcct atctgaggta aaccatttct aaagtaaaaa tttttattat
     1501 caatagtttt ataaccattc gcacccatag ctgtattagg ctcgaagtaa tatctattac
     1561 catcaatagt tacccaacca gtagccgctt ttgaattatt accaaaataa tatatattgt
     1621 catgtaaata taggaatcta ttttgataac gtatagcttg accttctata ttgttagcat
     1681 ctgtattagc aggtgcaaag tattcaaatc catcaggtcc tttaaacact cctatctgca
     1741 taataccatc agtattaaaa taaaaatgtt taccactaat aattgtataa ccagttgaag
     1801 ctatagaagt gttagtatta aagtagtatt ttttaccatt aatagtttgc catccagtaa
     1861 ctgcaacagc agtgttagta ttaaagtaat attttttacc atcaatagtt cgcaatccgg
     1921 taactgcttt tgagtcacta ccaaagtaat attttttacc attcaaagtt aagaatttat
     1981 tttggtaaag tatagcttga ccttctatgt tattattatg agtattagca ggtgcaaagt
     2041 attcaaatcc attaggtcct ttaaacactc ctatctgcat aataccatca gtattaaaat
     2101 aaaaatgttt accattaata cttgtataac cagttgaggc tatgaaagtg ttag
//
LOCUS       DAYAPA010000243         2059 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.248, whole genome shotgun sequence.
ACCESSION   DAYAPA010000243 DAYAPA010000000
VERSION     DAYAPA010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.248"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            160..774
                     /locus_tag="ACGI59_003789"
     CDS             160..774
                     /locus_tag="ACGI59_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HFG9741535.1"
                     /translation="MEEVIAKIITIENGFKEIENIAKKIVKNNDSKKCYYLSIYLYRS
                     EYYQVRELAVFILGFISVTISEALLFLKDNVSKDENWRVQEILAKSFDYYCSEVGYEK
                     ALPVIKEWLNSDSPNIKRAVTEGLRIWTSRDYFKSNPDVAISLLSSLKEDDSEYLRKS
                     VGNALRDISKKYPDLVKKELDSWDISNKKVEFVYKLASKCILAK"
     gene            792..1154
                     /locus_tag="ACGI59_003790"
     CDS             792..1154
                     /locus_tag="ACGI59_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFG9741536.1"
                     /translation="MKLRDEYTCPLELTHDMIKGKWKSIILWRLRIGPTSLSKLEKEI
                     NGINQKMLLEQLKELIEFGLVDKKTYEGYPLKVEYSLTPIRGKQILDALAIMQDIGIS
                     FMLDNGMEDILKEKGVLK"
     gene            1260..1757
                     /locus_tag="ACGI59_003791"
     CDS             1260..1757
                     /locus_tag="ACGI59_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HFG9741537.1"
                     /translation="MKVTSTGIVNGVIEDKYGKRGTQFNEGGMPTYSLPLKFEDAPKN
                     TVSFAVFLEDKDSVPVCGFAWIHWLAVNITKDELNENESVTATDFVQGTTSWHGKLGG
                     LDRLEASVYGGMAPPDRPHVYEIHVFALDTILDLEKGFYMNEFFKAIEGHVLDTFTLK
                     GSYSN"
BASE COUNT          783 a          228 c          338 g          710 t
ORIGIN      
        1 atgttaaatg aggttgattt tttattattt tttatttatg ctagtactaa agacttgcaa
       61 acgttttttg acaagtcttt attttgtcta aattaaacaa acttttttac aaataaatac
      121 ataaaattta aactaatcta tttattggag gagataaaat tggaagaagt aattgcaaaa
      181 attattacta tagaaaatgg attcaaagaa attgaaaaca ttgctaaaaa aattgtaaaa
      241 aataatgact caaaaaagtg ttattatcta tcaatctact tatataggtc agaatattat
      301 caagtaagag aattggctgt gtttatactt ggttttatat cagtaactat ttctgaagct
      361 ttgttatttt taaaagataa tgtgagtaaa gatgaaaact ggagagtaca agaaatacta
      421 gctaaatcat ttgattatta ttgcagtgaa gtaggatatg aaaaggctct tcctgttata
      481 aaagaatggc ttaattctga tagtccaaac ataaaaaggg ctgtgacaga aggtcttcga
      541 atttggacta gtagagacta ttttaaatct aatcctgacg ttgctatatc actactttct
      601 agtttaaaag aagatgatag tgaatacttg agaaagtctg taggtaatgc tcttagagat
      661 ataagtaaaa aatatccaga tttagtaaaa aaagaattag attcatggga tatatcaaat
      721 aaaaaagttg agtttgtata taagcttgct tcaaaatgta tcttggcaaa gtaaaatgga
      781 ggtgaactta tttgaaactt cgtgatgagt atacttgccc attagaacta acacatgata
      841 tgataaaagg caaatggaaa tctattatat tatggagatt acgcatagga ccaacttcct
      901 tatcaaaact tgaaaaggaa atcaatggta ttaatcaaaa gatgttactt gaacaactta
      961 aagaattaat tgaatttggt cttgtagata aaaagacata tgaaggttac ccacttaaag
     1021 ttgaatactc acttacacct attcgaggga aacaaatatt ggatgctttg gctataatgc
     1081 aagatatagg aattagcttt atgttggata atggtatgga agatattctt aaggaaaaag
     1141 gtgttttaaa ataaatagat acccacaaaa aagtaagtaa tttaccatta ttttcaactg
     1201 gtatataatt aaattataga tatattctat taatttatta taataggagg taaattatta
     1261 tgaaagtaac tagtacaggt atagtaaatg gtgttataga agataaatac ggtaaaagag
     1321 gaactcagtt caatgaaggt ggtatgccta catattcact acctctaaag tttgaagatg
     1381 ctccaaagaa tacagtatcc tttgctgtat ttttagaaga taaagactct gttcctgtat
     1441 gtgggtttgc atggatacat tggttagctg taaatataac aaaagatgag ttaaatgaaa
     1501 atgaaagtgt aacagctact gattttgtac aagggactac tagttggcat ggcaaactag
     1561 gaggtcttga cagattagag gccagtgtat atggtggaat ggctcctcca gacagacctc
     1621 atgtctatga aatacatgta tttgcacttg atactatcct tgaccttgaa aaaggattct
     1681 atatgaatga atttttcaaa gctattgaag ggcatgtttt agatacattt acactaaaag
     1741 gaagttattc taattaatta ggttgactat agctaatcat taattaaatg ctacatttaa
     1801 ttgatgatta gcttaattgt tattagaata ttttctgcac tttatcttaa tatatatttt
     1861 agttgactgg tattttaatt tctattagat tattatcaat ttatataaat tgattttagc
     1921 ttaaaactta cttatctatt tcagtttcta ttagaattat tattagactc attaaaataa
     1981 aagcactaat ataactaata atatatgtta aattcattaa ctctacataa agttttataa
     2041 tctaaatgaa atacaataa
//
LOCUS       DAYAPA010000244         2038 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.249, whole genome shotgun sequence.
ACCESSION   DAYAPA010000244 DAYAPA010000000
VERSION     DAYAPA010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.249"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            215..499
                     /locus_tag="ACGI59_003792"
     CDS             215..499
                     /locus_tag="ACGI59_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9741538.1"
                     /translation="MLVYNKSFYPNDIFPRLDFSKIKKQLKLIDNDLSDFGSICIIEK
                     EHYTISVNSIGEINVYYDLEYENKVYRIVYEIEKLFKSQVGRFSISTYRN"
     gene            complement(1270..1485)
                     /locus_tag="ACGI59_003793"
     CDS             complement(1270..1485)
                     /locus_tag="ACGI59_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFG9741539.1"
                     /translation="MAIVVNLDVMMAKRKISLKDLAEKIDITNANLSILKTGKAKAIR
                     FTTLNEICKALDCQPGDILEYVEDEND"
     gene            complement(1496..1963)
                     /locus_tag="ACGI59_003794"
     CDS             complement(1496..1963)
                     /locus_tag="ACGI59_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HFG9741540.1"
                     /translation="MITKFFSNILSVFLYFLLSLLGVAGIIFLCMIPMFLYDFSSDKV
                     IALIARILYFSDYFIVILFLIFIINSTKYSPFVLENVKRFKVMGFCLLINTIIECING
                     YNLNTNNIRIFGTDSGGISPLMIISFIFALMCFVIAETFDKAIKIKEDNDLTI"
BASE COUNT          826 a          210 c          245 g          757 t
ORIGIN      
        1 ctatttttat atagctatta tttttttatt gttgtggatg aaaatatact tatattttac
       61 taatatacat ttaaaaatta taatttaaag cattaaaaaa taaatttgta ttttgtttta
      121 tataaagtaa tttgaagaat atggaaaaac ttgattagta cattattaat tagggtataa
      181 aatggtgtaa atagaatgat aaggggtgga tgaaatgctt gtatataata aaagttttta
      241 tcctaatgac atatttccaa gattagattt ttcaaaaata aaaaaacagt taaaattgat
      301 agataatgac ctgtcagatt ttggaagcat atgtataata gaaaaagaac attatacaat
      361 aagtgtaaac agtataggtg aaataaatgt gtattatgat ttagagtacg aaaataaggt
      421 gtatagaata gtttatgaga ttgaaaagtt atttaaatct caagttggaa ggtttagcat
      481 atctacatac agaaattgat aattaaaaaa gtagaaatta aaaaacttaa tactaaagat
      541 atagataaat atatgaggta acagctagag agagttgtta ccctttttaa atactaagta
      601 tatattattc ttattaattt tttgtaaatt cactaaaaga cctttttata gaatattatt
      661 ttcttttttt attaaatagg taaatcagta aaaatgcaat tccagttcca attattattc
      721 caatgatatt ttctataatt aattttttta atataaaagt aaataataaa aatgctatac
      781 ataaacctaa aatatatcct ttaaaatagg ttgaaagttt tcttttcata taatcaccaa
      841 cttgtatcag atttagttta cttaattaga gtataacagt gttgtaactt caaagagtat
      901 ctaaaagatt acaaatgtta gataaaatca tcaataatgt aatttataac acgtagctat
      961 aaactatgca ataatataaa tggaggtata agtaaatgaa aaagttccat gttttgctaa
     1021 cagtttaaat aaatattagg tagaaaagaa tgtatatagg aatctgtggt aaaattatca
     1081 atttaaaagt attatttagt gtgatacagt tcttataggt aatggataac aaatagataa
     1141 aggaacagtt tctttcttag aacatatttt gttatgactt taaacaaagt taatattaat
     1201 gtcaataata tagtaaaaga ataaaaatca tatagtataa taaaaaagag tgtttctaaa
     1261 cactcttttt taatcatttt catcttctac atattcaagt atatcacctg gctgacagtc
     1321 taaagctttg caaatttcgt ttagagtggt aaatctaata gcttttgctt ttccagtttt
     1381 taaaattgat aaatttgcat ttgtaatatc tattttttct gccaaatctt taagtgatat
     1441 tttccttttt gccatcatta catctaggtt aactactatt gccataacat cacctctata
     1501 tcgttaaatc attatcttct tttattttta tagctttatc aaatgtttct gcaattacaa
     1561 agcacattag tgcaaatata aaagagataa tcataagtgg tgagataccg ccactatcag
     1621 ttccaaaaat tcttatatta ttagtattta aattataacc attgatacat tcaattatag
     1681 tgtttatcaa taaacaaaat cccattacct taaacctttt tacattttca agtacgaatg
     1741 gggaatactt agtactatta attataaata tcaagaataa gatgactata aagtagtcag
     1801 aaaaatatag tattcttgca attaaagcga ttactttgtc agaactaaaa tcatataaaa
     1861 acataggtat catacataag aatattatac cagcaactcc taaaagtgat aatagaaaat
     1921 atagaaatac tgataatata ttactaaaaa attttgtaat cataaaatat catctccttt
     1981 atataaagta tattattgat tataatatac aatttattga atttcaataa taaattat
//
LOCUS       DAYAPA010000245         1977 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.250, whole genome shotgun sequence.
ACCESSION   DAYAPA010000245 DAYAPA010000000
VERSION     DAYAPA010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1977
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.250"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            20..418
                     /locus_tag="ACGI59_003795"
     CDS             20..418
                     /locus_tag="ACGI59_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9741541.1"
                     /translation="MNKKKKLIGIAISFLLIISIINPLQVEAEAIIESSVYISSIKEN
                     YTYDNTKFKTIKSKSRHKSRKYNKSKVKLKKRSIRTKKYGYQTINKYKRQNARRHNIL
                     TKLILSIIVIFLVIVLVVLIFIKRKKKKFY"
     misc_binding    854..1117
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1268..1819
                     /locus_tag="ACGI59_003796"
     CDS             1268..1819
                     /locus_tag="ACGI59_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan transporter"
                     /protein_id="HFG9741542.1"
                     /translation="MKTNTKKLTLNAILLAMGLLIHQITPAIGLPMKPDVPLAMLFVI
                     LVLNRDDYKTCLIAGIVTGIFTALTSSFPGGQIPNVIDKTLTANIVFLLMNISYNMPF
                     IKKLAKKTQDTVVVAIIMPIGTLVSGTIFLLAAQAIVGLPGASFTALFLAVVLPAVLI
                     NLVAGIFLFKIVSLSIRRVSYQA"
BASE COUNT          829 a          189 c          294 g          665 t
ORIGIN      
        1 tttttatgag ggggaaaata tgaataagaa gaaaaaattg attgggatag ctatatcttt
       61 tctacttata atcagcatta taaatccatt acaagtagaa gcagaagcta ttattgagtc
      121 tagtgtatac atttcttcaa taaaagagaa ttatacatat gataatacta aatttaaaac
      181 tattaaatct aaaagtagac ataaaagtag gaaatataat aaaagtaaag taaagttgaa
      241 aaaacgaagt ataagaacta aaaagtatgg atatcaaact ataaataaat ataaaagaca
      301 aaacgcaaga agacataata tcctaacaaa attaatattg tccataatag tcatattttt
      361 ggttatagtt ttagtagtgc taatatttat taaaagaaag aagaaaaaat tttactaaaa
      421 ataattaaaa gaatatcatt tttttaatca ttggtatcta tttaataata tgatgtaatt
      481 aaaatggagt aatattctgt ttcaaaggag gcatataaat acaggaatga gtattaaatg
      541 atggttaatt aagtaaattt attgataaat gtacttaatt aactttgaag aatttaattc
      601 ctcatgaatc aaaatataaa attacatcaa cagttattca caaaattaat ataagaagaa
      661 ttagagccaa agagcagact attagaccat taaagtaatt attcatagaa ttttgtatat
      721 gaaaagaaat gtatagtata atttactttt tttataaaaa atagattaca aaatatattt
      781 tttataaaaa ggtattgaca ttttttagaa aaaagaatat cattatttta acaacaaaat
      841 tcaatacaat aaattaaatc ctatgacaag gaagagtagt taaatttagg ttcagtatag
      901 agagctgagg atggtggaat cttagcaaga aaaaatttga tgaatggccc ttagaggaat
      961 aaattgaaat catagttttg agagtagact ttatcgtcag tcgcacgtta tagcgaataa
     1021 ggtatgtaag tacctgataa tgagatgtat gaaaattgaa ttttatatta atttttatat
     1081 aatttaggtg gcaacgcgga taatctccgt cctattagtt taggacggag ttttttattt
     1141 tactgaaaat ttaaaagata gaaggtggtg agaaagatgt tatgaatttc aaaagctaaa
     1201 gatgatgata tagtaggttg gcaaaaaaat aaattgaaaa ttaaaaaaat aggaggaagt
     1261 acaaaaaatg aaaacaaata caaaaaaatt aactttaaat gcaatacttt tagcgatggg
     1321 attattaata catcaaatca caccagctat aggattacca atgaagccag atgtaccact
     1381 agcaatgtta tttgtaatat tggttttaaa tagagatgat tataaaactt gtttgatagc
     1441 gggtatagta acaggaatat ttacagcact tacatcaagt tttccgggag gtcaaattcc
     1501 taatgtaata gataaaactc ttacagcaaa tatagtattt ttattaatga atattagtta
     1561 taatatgcct tttataaaaa aattagctaa gaaaacacaa gatactgtag tagttgcaat
     1621 aataatgcca ataggaactc ttgtaagtgg aactatcttt ttattagcag ctcaagctat
     1681 agttggtctt ccaggggctt catttacagc tttattctta gcagtagtat taccagctgt
     1741 tttaataaat ctagttgcag gaatattttt attcaagatt gttagtctat caataagaag
     1801 agttagttat caagcttaaa cacgaaataa tattttactt tataaagcat tataataact
     1861 aaataacata agaataaagt agattataac tattttattc ttatgttgtt taaagatttg
     1921 gtattatata tttattttta ataaaatatt tatatagtac caattacact taattta
//
LOCUS       DAYAPA010000246         1925 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.251, whole genome shotgun sequence.
ACCESSION   DAYAPA010000246 DAYAPA010000000
VERSION     DAYAPA010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1925
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.251"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            119..235
                     /gene="rrf"
                     /locus_tag="ACGI59_003797"
     rRNA            119..235
                     /gene="rrf"
                     /locus_tag="ACGI59_003797"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            242..316
                     /locus_tag="ACGI59_003798"
     tRNA            242..316
                     /locus_tag="ACGI59_003798"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:274..276,aa:Asn,seq:gtt)
     gene            321..395
                     /locus_tag="ACGI59_003799"
     tRNA            321..395
                     /locus_tag="ACGI59_003799"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:354..356,aa:Glu,seq:ttc)
     gene            401..476
                     /locus_tag="ACGI59_003800"
     tRNA            401..476
                     /locus_tag="ACGI59_003800"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:434..436,aa:Val,seq:tac)
     gene            482..558
                     /locus_tag="ACGI59_003801"
     tRNA            482..558
                     /locus_tag="ACGI59_003801"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:516..518,aa:Asp,seq:gtc)
     gene            569..643
                     /locus_tag="ACGI59_003802"
     tRNA            569..643
                     /locus_tag="ACGI59_003802"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:601..603,aa:Thr,seq:tgt)
     gene            658..742
                     /locus_tag="ACGI59_003803"
     tRNA            658..742
                     /locus_tag="ACGI59_003803"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:692..694,aa:Tyr,seq:gta)
     gene            752..825
                     /locus_tag="ACGI59_003804"
     tRNA            752..825
                     /locus_tag="ACGI59_003804"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:784..786,aa:Gly,seq:tcc)
     gene            836..912
                     /locus_tag="ACGI59_003805"
     tRNA            836..912
                     /locus_tag="ACGI59_003805"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:870..872,aa:Arg,seq:tct)
     gene            922..997
                     /locus_tag="ACGI59_003806"
     tRNA            922..997
                     /locus_tag="ACGI59_003806"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:955..957,aa:Gln,seq:ttg)
     gene            1009..1084
                     /locus_tag="ACGI59_003807"
     tRNA            1009..1084
                     /locus_tag="ACGI59_003807"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1042..1044,aa:Lys,seq:ttt)
     gene            1087..1175
                     /locus_tag="ACGI59_003808"
     tRNA            1087..1175
                     /locus_tag="ACGI59_003808"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1121..1123,aa:Ser,seq:tga)
     gene            1193..1283
                     /locus_tag="ACGI59_003809"
     tRNA            1193..1283
                     /locus_tag="ACGI59_003809"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1227..1229,aa:Ser,seq:gct)
     gene            1292..1368
                     /locus_tag="ACGI59_003810"
     tRNA            1292..1368
                     /locus_tag="ACGI59_003810"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1326..1328,aa:Pro,seq:tgg)
     gene            1454..1529
                     /locus_tag="ACGI59_003811"
     tRNA            1454..1529
                     /locus_tag="ACGI59_003811"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1487..1489,aa:Trp,seq:cca)
     gene            1590..1666
                     /locus_tag="ACGI59_003812"
     tRNA            1590..1666
                     /locus_tag="ACGI59_003812"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1624..1626,aa:Pro,seq:tgg)
     gene            1673..1749
                     /locus_tag="ACGI59_003813"
     tRNA            1673..1749
                     /locus_tag="ACGI59_003813"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1707..1709,aa:Ile,seq:gat)
     gene            1753..1828
                     /locus_tag="ACGI59_003814"
     tRNA            1753..1828
                     /locus_tag="ACGI59_003814"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1786..1788,aa:Phe,seq:gaa)
     gene            1836..1912
                     /locus_tag="ACGI59_003815"
     tRNA            1836..1912
                     /locus_tag="ACGI59_003815"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1870..1872,aa:Met,seq:cat)
BASE COUNT          484 a          442 c          524 g          475 t
ORIGIN      
        1 attcattaag atatatgtgc agtttttaga gtgttaactc taaaaaaaaa taaaaaaagt
       61 attgacaaaa atagtaaaaa atgttaatat tatacttgtc ctagcaacaa gaaaaaaatg
      121 tggttataat agcggagaag atacacctgt ttccattccg aacacagaag ttaagttctc
      181 tagcgctgat ggtacttggt gggaaactgc ctgggagagt aggacatagc cacgtgaatg
      241 ttccaaggta gctcaacggt ggagcaaccg gctgttaacc ggtaggctgt gggttcgagc
      301 cccacccttg gagccattat ggctcgttgg tcaagaggtt aagacaccgc cctttcacgg
      361 cggtaacagg ggttcaattc ccctacgagt caccatttgt gggatcatag ctcagctggg
      421 agagcacctg ccttacaagc agggggtcac aggttcgagc cctgttggtc ccaccatttg
      481 cggcctggta gttcagttgg ttagaatgcc agcctgtcac gctggaggtc gagggttcga
      541 gccccttcca ggtcgccaaa ataaaaatgc tggtgtggct caacggtaga gcagctgact
      601 tgtaatcagc aggttgtagg ttcgattcct atcaccagct ccatgtaaaa ctaatacgga
      661 ggatttcccg agcggccaaa gggggcagac tgtaaatctg ttgtcatcga cttcggtggt
      721 tcgaatccac catcctccac caattaaata tgcgggtgta gctcaatggt agagttctgg
      781 ccttccaagc cagctgtgag ggttcgatcc ccttcacccg ctccaaagaa agcgtgggtg
      841 catagctcag ctggatagag caacgccctt ctaaggcgtg tgtccggggt tcgaatccct
      901 gtgcgctcac cacatatata ttggggatta gccaagtcgg taaggcacat gactttgact
      961 catgtatgcg taggttcgag tcctgcatcc ccagccaaat aaaaatatgg tctattagct
     1021 cagtcggtag agcacctgac ttttaatcag ggtgtcccgc gttcgagtcg cggatagatc
     1081 accaatggag aggtgtccga gtggtttaag gagctggtct tgaaaaccag tgacttcgaa
     1141 aggggccgtg ggttcgaatc ccaccctctc cgccaaaaaa atgactgaat ttggagaagt
     1201 actcaagtgg ctcaagagga tcccctgcta agggattagg ctgggtaacc ggtgcgaggg
     1261 ttcgaatccc tccttctccg ccattcaaaa acgaggtgta gcgcagtttg gtagcgcaca
     1321 tggtttggga ccatggggcc gggggttcga gtcccttcac ctcgaccaac ttaagctttt
     1381 agttcagttg gttagagtgc ccaactcatg gctagtaatt ctagggtttg agtccatgaa
     1441 ggttattctt cataggggtg tagctcagtt ggtagagcgt cggtctccaa aaccgtgcgc
     1501 cgggggttcg agtccctcca cccctgccaa ataaaaagga cttgaaaaaa gttggatttt
     1561 agagtagtat aagatagtaa ataagatttc gaggtgtagc gcagtttggt agcgcacatg
     1621 gtttgggacc atggggccgg gggttcgagt cccttcacct cgaccaagaa aagggcctat
     1681 agctcaggtg gttagagcgc acgcctgata agcgtgaggt cgctggttcg agtccagcta
     1741 ggcccaccat ttgcccagat agctcagtcg gtagagcagg ggactgaaaa tccccgtgtc
     1801 ggtggttcga ttccgcctct gggcaccagg aatacggcgg tatagcttag ttggctagag
     1861 cgttcggttc atacccgaaa ggtcataggt tcgactccta ttaccgctac catctaacta
     1921 tatca
//
LOCUS       DAYAPA010000247         1841 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.252, whole genome shotgun sequence.
ACCESSION   DAYAPA010000247 DAYAPA010000000
VERSION     DAYAPA010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1841
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.252"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            2..1135
                     /locus_tag="ACGI59_003816"
     CDS             2..1135
                     /locus_tag="ACGI59_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HFG9741543.1"
                     /translation="MKLTIEEYKNKYFTGNKIVLSINSFDNKELYYHETENILNLINE
                     IDKLTSSKIFKTIEDKNDLALESYYSSVIEGAFSTRKIAKSIIRGKMKPSNKSEYMIY
                     NNHRALEYGLDNLDELYSHKFIYDLHHILGENCLDSEEYEYRTEKVYVCDSKGEIIHT
                     GLEPLKIYDFISKLIDFMENSKVSNLIKSAIIHFYFVYVHPFSDGNGRTSRALSYLYL
                     IDKGYDTFKDFSISYMISKNRTKYYKAILDVENKGNNLTVFIEFMLKSIIQSINEMRN
                     MHDRKSLESILKEELFENDITLSATEEHILKYICNKDNYSMTLENYIKKNKSRYLKAG
                     IKEIELVDQLMEVFNNLEEIEILSKEKNIYKVNDKYLKMLDID"
     gene            1161..1349
                     /locus_tag="ACGI59_003817"
     CDS             1161..1349
                     /locus_tag="ACGI59_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9741544.1"
                     /translation="MNNKKKAFLISGAINVISLIIVFSLFHYNIIENSTQNMIITVIL
                     IFIFEMIKIKIIEKYYKL"
BASE COUNT          786 a          149 c          267 g          639 t
ORIGIN      
        1 gattaaactg acaatagaag aatacaaaaa caaatacttt actggaaata aaatcgtact
       61 ttctataaat agttttgata ataaagaact atactatcat gaaactgaaa atatattaaa
      121 tttaataaat gaaatagata aattaacatc aagtaagata tttaaaacaa tagaagataa
      181 aaatgatttg gcactagagt cttattattc tagtgtaata gaaggtgctt tttcaactag
      241 aaagattgca aaatctataa ttagaggaaa gatgaaacca agtaataaaa gtgaatatat
      301 gatttacaat aatcatagag ccttagaata tggattagat aacttagatg aattgtatag
      361 tcacaaattt atatatgatt tgcatcatat attgggcgaa aactgcttgg attcagaaga
      421 atatgagtat agaactgaaa aagtatatgt atgtgattca aaaggtgaga ttatacatac
      481 agggcttgaa cctttgaaga tatatgactt tattagtaaa ttaatagatt ttatggaaaa
      541 tagcaaagta agtaatttaa ttaaaagtgc tataattcat ttttactttg tatatgttca
      601 tccttttagt gatggtaatg gaagaacatc aagagcctta tcatatctat atttgattga
      661 taaaggatat gatactttca aggatttttc aatatcatat atgatatcta aaaacagaac
      721 taagtattat aaggctatac ttgatgtaga aaacaaagga aataatttaa ctgtatttat
      781 agaatttatg ctcaagtcta taatacaatc tattaatgag atgcgtaata tgcatgatag
      841 aaaatcttta gaaagtatat taaaagaaga attgtttgaa aatgacataa ctctatcagc
      901 aacagaagaa catatattaa aatatatttg caataaagat aactattcta tgacattaga
      961 aaattatata aaaaagaata aaagtagata cttaaaggct ggaataaagg aaattgaatt
     1021 agttgaccag ttgatggaag tgtttaataa tcttgaagag atagagattt tgtctaaaga
     1081 aaaaaatata tataaagtta atgataagta cttaaaaatg cttgatatag actaatttag
     1141 ataatctgga gagggaatat atgaataata agaaaaaggc atttttgatt tctggggcta
     1201 taaacgtaat ttcattaatt attgtttttt cattgtttca ttataatatt atagaaaata
     1261 gtacacaaaa tatgataata actgtaatct taatttttat ttttgaaatg ataaaaataa
     1321 aaatcataga gaaatactat aaactataaa caagaaatat cttttctaag agcatattgt
     1381 acaatatgct cttaatttag ttatatagat ttaatataaa taatgtattt tttgtagatg
     1441 atttatgtaa gatttatgac caatttgtgt atgatttgtg ttctttaatc atgacaatat
     1501 tatattgtaa aaaataaatt taataaaaaa tgacttggaa tttatattct aggtcttttt
     1561 tatataaaaa tttaataagt ttcaaatgtt ggtaactttg acagtagaaa acgtctattg
     1621 agataacgag gattctaatt gaggtggaga gatattagca cttaaagtag tatatgcaac
     1681 agatataatc tttacaaaag taatttgaat taaaatgtaa atataaagaa taattaaaaa
     1741 aatatcgcaa ttatttgttt ttcatatgga taaaattggt tatttaatat ataattacaa
     1801 gtataaataa taaaatttat ttgtaaaaag agtagcactt t
//
LOCUS       DAYAPA010000248         1814 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.253, whole genome shotgun sequence.
ACCESSION   DAYAPA010000248 DAYAPA010000000
VERSION     DAYAPA010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1814
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.253"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            82..210
                     /locus_tag="ACGI59_003818"
     CDS             82..210
                     /locus_tag="ACGI59_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9741545.1"
                     /translation="MELISPGKIVRCVWCGKRFYKLDNSKVIYCSRKCAAKARGVF"
     gene            211..>1814
                     /locus_tag="ACGI59_003819"
     CDS             211..>1814
                     /locus_tag="ACGI59_003819"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9741546.1"
                     /translation="MIFYDFEVFSYDWLVVFIDVLNKKEEVIVNDIDKLNSFYIEHRE
                     DIFIGYNSRHYDQYIFKGLLCGFNAKEINDYIIVKGQPGWKFSNLLRNIQINNYDVMT
                     SFHGLKQLEGFQGHSIKESNVSFNIDRPLTNDEIEETIKYCRYDVEQTIDVFIERKSE
                     FEAHMGLIKAFKLPVSYIGKTQTQLTAIILEATKKEHDDEFDLQIPDTLKIEKYKEVL
                     SWYKNPLNHDYSKNLKINISNVPHVFAWGGVHGAITKYYGEGYFLHVDVNSFYPSLMI
                     RYNYHSRNIKNPQKYVEIYDKNLQLKKEKSPLRPAYKLAVNKTYGGMKDKNNNLYDPR
                     QANNVCVSGQLLLLDLIEKLEGYCKLIQSNTDGLIVKLNTIDDYELIDDICYEWEQRT
                     GMGLGFDVYTKIFQKDVNNYLVITEDGEVEAKGLYIKDLGNLDYDLPIINKALKNYMV
                     NNTPIEETINKCNDLIEFQKIVKISSKYSHGLYSPTIEGKTKKVFIGGKILNDKCFRV
                     FASKNKNDGGIYKVKNKEKNPEKFANT"
BASE COUNT          759 a          176 c          302 g          577 t
ORIGIN      
        1 ttttattaca gctaggtgta tacatgcatc tagctgtaat attaaaaagg taggtaatta
       61 atatgagagt aaaaagaaaa aatggaactt ataagcccag gtaaaattgt aagatgtgtt
      121 tggtgtggaa aaagatttta caagttagat aactctaaag tcatatattg tagtagaaaa
      181 tgtgcagcaa aagcgagagg ggtgttctaa atgatttttt atgattttga agtattctct
      241 tatgactggt tagttgtatt tatagatgtt ttaaataaaa aagaagaagt tattgtaaat
      301 gatatagata agttaaattc attttatata gagcatagag aagatatttt tattggttac
      361 aacagtagac attatgacca atatattttt aaaggattat tatgtggatt taatgcaaaa
      421 gaaataaatg attatattat tgttaaaggt caacctggtt ggaagttttc aaacttatta
      481 agaaacatac aaattaataa ctatgatgtt atgactagtt ttcatggatt aaaacaatta
      541 gaaggttttc aaggtcatag tataaaagaa tctaatgtat catttaacat tgatagacca
      601 ttaactaatg atgaaataga agaaactata aaatactgta gatatgatgt cgaacaaact
      661 atagatgttt ttattgaaag aaaatccgaa tttgaagcac atatgggatt aattaaagct
      721 tttaaacttc cagtatcata tattggtaaa actcaaacac aactaacagc aattatatta
      781 gaagctacaa agaaagaaca tgatgatgaa tttgacttac aaataccaga tactctaaaa
      841 attgagaaat ataaggaagt tttaagctgg tataagaacc cactaaatca tgactattct
      901 aaaaatctaa aaattaatat ttctaatgta cctcacgtat ttgcttgggg tggtgttcat
      961 ggagcaataa caaaatacta tggtgaaggt tattttttac atgtggatgt taattccttc
     1021 tatccaagtc ttatgattag atataattac cactcaagaa atataaaaaa tcctcaaaaa
     1081 tacgttgaaa tttatgacaa aaacttacag ttaaaaaaag aaaaatcacc tctaagacca
     1141 gcctacaaat tggcagttaa taagacttat ggaggaatga aagataaaaa caataattta
     1201 tatgacccaa gacaggctaa taatgtatgt gttagtggac agctgttatt acttgattta
     1261 atagagaagt tagaaggtta ttgtaaactt attcagagca atacagatgg attaattgta
     1321 aagttaaata caatagatga ttacgaatta atagatgata tatgttatga gtgggaacaa
     1381 aggacaggta tgggtttagg ttttgatgta tacactaaaa tatttcaaaa ggatgttaac
     1441 aattatttag ttataacaga agatggagaa gttgaagcaa aaggattata tatcaaagat
     1501 ttaggaaatc tagactatga tttaccaatt ataaataaag cacttaaaaa ttatatggta
     1561 aacaatacac ctattgaaga aactataaat aaatgtaatg atttaataga gtttcaaaag
     1621 atagtaaaaa taagtagtaa atattcacat ggtttatata gtccaactat agagggaaaa
     1681 acaaaaaaag tatttattgg cggtaagatt ttaaatgata aatgttttag agtatttgct
     1741 tcaaaaaata aaaatgatgg tggtatttat aaagtcaaaa ataaagaaaa aaatccagaa
     1801 aaatttgcta atac
//
LOCUS       DAYAPA010000249         1765 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.254, whole genome shotgun sequence.
ACCESSION   DAYAPA010000249 DAYAPA010000000
VERSION     DAYAPA010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1765
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.254"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            130..1728
                     /locus_tag="ACGI59_003820"
     CDS             130..1728
                     /locus_tag="ACGI59_003820"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese-dependent inorganic
                     diphosphatase"
                     /protein_id="HFG9741547.1"
                     /translation="MSLLVFGHKNPDTDSICSAISLAHLKNKLGIKATAYALGDIRKE
                     AKYALDYFKVDAPEILDNVRIQVRDLNYDKVVPLTPTSSILEAYNLMDEKNVKTLPVT
                     YDDGTFAGIITMKEIAKNLLHQHFTTIHTSLSNICKNLNGTILVDCGEDIKGRVVTLS
                     FGMETLIETLKEGDIAIVGDRYDSIEYAIDTKVKLLILTNHTEISKDLLALAKINGVS
                     VVSVESDNYKTSNVINQCSFLDSIEATENLITFKEDDYMDEIKEIMLETNFRSYPILD
                     DDNKFLGLVSKGHLLNPSKKNVVLVDHNEYAQSADGIEQANIVEIIDHHKLGGISTDV
                     PMSFRVMPVGCNSTIIYQMYKENNVEIPYEIAGLLLSAILSDTLLFKSPTTTDIDKKA
                     CEELSKIAKVDMEKYAMDMFKCGTSLDEYTIEEIVNMDFKEFNMSGHRVGIGQVFTLD
                     IDSIFAKKDEFLSYINSTDYDKLILAVTDIIKEGSYLIYKAEDSLIANAFGVEGVQGT
                     FAPGVVSRKKQLVPNLTTAIKNFK"
BASE COUNT          677 a          220 c          273 g          595 t
ORIGIN      
        1 tgtaagaaat aaattttata taatattttt tcactaaatt tttttctttt tacaacaaat
       61 actcactacc ttttgatata atatttttat caattattat tatattcaag ttttgaaagg
      121 agtattaaaa tgagtttatt agttttcgga cataaaaatc cagatacaga ttcaatatgt
      181 tctgcaattt cattagcaca tttaaagaac aaactaggaa taaaagcaac agcttatgct
      241 ttaggagata tcagaaaaga agctaaatat gccttggatt actttaaggt agatgcacct
      301 gaaattttag ataatgttag aatacaagtt agagatttaa attatgataa agtagtacct
      361 ttaacaccaa cttcatctat attagaggct tataacctta tggatgaaaa aaatgttaaa
      421 acactacctg taacttatga tgatggaact tttgctggta tcattactat gaaagaaata
      481 gctaaaaatc tacttcatca acactttaca actatacaca catcactatc aaacatttgt
      541 aaaaacctta atggtactat tcttgtggac tgtggtgaag atataaaagg tagagtagtc
      601 actttatcat ttggaatgga gactctaata gagactttaa aagaaggaga catagctatt
      661 gttggagata gatatgatag tattgaatat gctattgata caaaagttaa acttttaata
      721 cttactaatc atacagagat ttcaaaagat ttgctagctc ttgctaagat aaatggagtg
      781 tctgttgttt ctgttgaaag tgacaattat aaaacttcta atgtaataaa tcaatgtagt
      841 ttcttagact ctatagaagc tacagaaaat ttaattacat tcaaagaaga tgattatatg
      901 gatgaaataa aagagattat gctagaaaca aacttcagat cttatccaat attagatgat
      961 gataataagt ttttaggttt agtttcaaaa ggtcatctat taaacccatc taaaaagaat
     1021 gttgttttag ttgaccataa cgaatatgct caaagtgctg atggaattga acaagcaaac
     1081 atagttgaaa taattgacca tcataaactt ggtggtattt ctactgatgt tcctatgtca
     1141 tttagagtta tgcctgttgg atgcaatagt actataattt atcaaatgta caaagaaaat
     1201 aatgttgaga taccttatga aatagccggg cttcttttat ctgctatatt atcagataca
     1261 ttattattta agtctccaac aacaactgat atagataaga aagcttgtga agaattaagt
     1321 aaaatagcaa aagtagacat ggaaaaatac gctatggata tgtttaaatg cggtacttct
     1381 ttagatgaat atactataga agaaatagta aatatggact ttaaagaatt taatatgagt
     1441 ggacatagag ttggtattgg acaagtattt accttagaca tagactctat atttgctaaa
     1501 aaagatgagt ttttatctta tataaattcg actgattatg acaaactaat tcttgcggtt
     1561 acagatataa tcaaagaagg ttcttattta atctataaag cagaagacag cttaattgct
     1621 aacgcatttg gtgttgaagg tgttcaaggt acatttgcac caggtgttgt atcaagaaaa
     1681 aaacaattag tccctaattt aactactgca atcaaaaatt ttaaataata taaaattttt
     1741 aaataacgaa aactgtctca aatct
//
LOCUS       DAYAPA010000250         1695 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.255, whole genome shotgun sequence.
ACCESSION   DAYAPA010000250 DAYAPA010000000
VERSION     DAYAPA010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1695
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.255"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(<1..165)
                     /locus_tag="ACGI59_003821"
     CDS             complement(<1..165)
                     /locus_tag="ACGI59_003821"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFG9741548.1"
                     /translation="MFFSLFKKKSGYTFTNFLSKVRVEKSKKYILKKDLSILDVAVLI
                     GFNSQNYYSII"
     gene            458..649
                     /locus_tag="ACGI59_003822"
     CDS             458..649
                     /locus_tag="ACGI59_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9741549.1"
                     /translation="MERLMELIERKFLKDVELLELEDIENVIECKMTQPYNAHYNEYL
                     VKVKNGDEVEEYFVYLKHR"
     gene            complement(738..1550)
                     /locus_tag="ACGI59_003823"
     CDS             complement(738..1550)
                     /locus_tag="ACGI59_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HFG9741550.1"
                     /translation="MADCNSCPSKGNCNSQSNCSIENNPNNKFGKIIGVMSGKGGVGK
                     STVTALLANKLNKMGYKVGILDSDITGPSIPRLMGVKNVKAYSDGSYIYPVENSNNIK
                     VMSINLMIDDENEPVVWRGPLLGGVVKQFYTDVLWEELDYLLIDMPPGTGDVALTVMQ
                     SIPISGIVMVSVPQDLVSMIVSKAVNMAKKMNINVLGVIENMSYIQCPDCSKKIKLFE
                     GESTEKFLDDLDLELLGELPMTKEIIDITHNGVTEISDDLDSILTNVVEKIK"
BASE COUNT          629 a          238 c          195 g          633 t
ORIGIN      
        1 aattatacta tagtaatttt gactattgaa tccaattagt acagcaacgt ctaaaataga
       61 caagtctttt tttaatatat actttttgct tttttcaact ctaactttac ttaaaaaatt
      121 agtaaaagta tatccacttt ttttcttaaa taaagaaaaa aacaactttt attaagttgt
      181 aattcattac atatattact tactgttata tcttctgagt aatttttgtg aatataatct
      241 atagttttct ttaagttttc tttacataaa actaagagtt ggtctatgtt tcattttatc
      301 aaggcatatt tattaaaaaa tcaatctcta tttgttaaaa taatttatga atgatgttag
      361 aaactgtaat atattggata taatataata aacagataaa ctctgtatta agaggatata
      421 tagtttatct taattatagg ggaagtgagg atatattatg gaaagattaa tggaactcat
      481 agaaagaaaa tttttaaagg atgtagaact tttagaatta gaagacatag aaaatgtaat
      541 agaatgcaaa atgacacagc catataatgc tcattataat gaatatttag taaaagtaaa
      601 aaatggagat gaagtagaag aatattttgt gtatttaaaa catagataaa cataaaagag
      661 gtttatattt atttgtatga ttataaaata aatacaagcc tctttttttg ataaatataa
      721 aattataaaa tttttattta tttaattttt tcaacaacat tagtcaatat tgaatctaaa
      781 tcatcactaa tttctgtaac tccattatga gtaatatcta taatttcttt agtcatagga
      841 agttctccta aaagctctaa gtctaaatca tctaaaaact tttcagtaga ttctccttca
      901 aataacttga tttttttaga acaatcagga cattggatat aactcatatt ttcaataact
      961 ccaaggacat ttatgttcat ttttttagcc atattaacag cttttgatac aatcatagat
     1021 accaaatctt gaggtactga aaccataact attccactta taggaataga ttgcataact
     1081 gttaaggcaa catctccagt accaggaggc atatctatta ataaatagtc taattcttcc
     1141 caaagaacat cagtataaaa ttgctttaca acccctccaa gtaaaggtcc tctccaaaca
     1201 acaggttcat tttcatcatc tatcataagg tttatagaca ttacttttat gttatttgag
     1261 ttttctacag ggtaaatata agagccatca gaataagctt ttacattttt tacacccata
     1321 agtctaggaa tacttggtcc agttatatct gagtctaata taccaacctt atatcccatt
     1381 ttgtttagct tattagcaag taaagcagtt acagttgatt ttccaactcc acccttccca
     1441 ctcataacac caattatttt tccaaattta ttgttagggt tattttcaat agaacaattt
     1501 gattgactat tacaatttcc ctttgatgga catgaattac aatctgccat atctattacc
     1561 tccaaacttt aatatttatt agattatttg taacctacat atactatttt agtacaattg
     1621 aaccataaag tcattattta aaagcaactt agaagatttt ctattgattt attcatagac
     1681 tatagcaaaa agaat
//
LOCUS       DAYAPA010000251         1665 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.256, whole genome shotgun sequence.
ACCESSION   DAYAPA010000251 DAYAPA010000000
VERSION     DAYAPA010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1665
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.256"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            263..544
                     /locus_tag="ACGI59_003824"
     CDS             263..544
                     /locus_tag="ACGI59_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9741551.1"
                     /translation="MASKQIPRNNLEKFLRIRVLSHTGDKIHIKLPVNFAKRMIENNA
                     LDLFNGKDDVVDGKKVTEILVKAFDYDLTGEVVHLERKNGDIIKVSIVQ"
     gene            808..1062
                     /locus_tag="ACGI59_003825"
     CDS             808..1062
                     /locus_tag="ACGI59_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9741552.1"
                     /translation="MENKDFKLTICIFAFVIGVVVLFCCIGLGEFSAEHIIQQNGGIM
                     DTDQYNIYLDQSISQYRNFGSILALLGGIGILFDKYIRKY"
     gene            1107..1430
                     /locus_tag="ACGI59_003826"
     CDS             1107..1430
                     /locus_tag="ACGI59_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6054 family protein"
                     /protein_id="HFG9741553.1"
                     /translation="MATEIFTVKITPLEAQKMIKENLDADLVFSDSYNLGDDKFILIT
                     TFEKYYARNNSDAGLIVVYENTTGKTVIKATSTGSAIGILKIDWGAGGNLLKRVKNIL
                     ADYII"
BASE COUNT          715 a          120 c          248 g          582 t
ORIGIN      
        1 tataataaac cagttttgtt ttaattgaaa tattctaata agatattttg ttaaatttag
       61 atacaataat tgtaaagtaa aatctcaaaa tatacaatta ttataaatca tagatattta
      121 ttaaataaat atattaaaga agaaggaata taaaacaaga ttagaaaata atagtgttac
      181 tttcataata aatacatata aagatatttt taagaataaa atttattatt aaataaaaaa
      241 taaaggagag tgatttaata gcatggcatc aaaacaaata ccaagaaaca atttagaaaa
      301 gtttttaaga ataagagtct tatcacatac aggcgataaa atacatataa agttacctgt
      361 aaattttgca aagagaatga ttgagaataa tgctttggat ttatttaatg gaaaagatga
      421 tgtagtagat ggtaaaaaag ttactgaaat attagttaag gcatttgact atgatttaac
      481 tggagaagta gttcacttgg agagaaaaaa tggagacatt attaaagttt caattgtaca
      541 atagaattat tttatatgaa ttttattttt gaatagttat aaataattag aaatttgtaa
      601 aaaagtataa tatattatta gtaatggtac agaatacaca tttataattt atagatgtgt
      661 attttttgtt taaaaaattt aaatatataa aactataaag gttacaattt tggaaataga
      721 tgtaaatata tgttgtattg tataataaga gtaatataaa atctagtaaa aataatcaaa
      781 taaatttagc ttatgagagg gataactatg gaaaataagg attttaaatt aacaatatgt
      841 atatttgctt ttgtaattgg agtagtagta ttattctgtt gtataggttt aggtgaattt
      901 agtgcagagc atattattca acaaaatggt ggaatcatgg atactgacca atataatatt
      961 tatctagacc aatctatttc tcaatataga aattttggtt ctatattagc tctgttagga
     1021 ggaataggga tattgtttga taaatatatc agaaaatact agagattatg ttttgtctaa
     1081 aataaattct tatgaggaga taaaaaatgg caacagaaat atttactgta aaaattacac
     1141 ccttagaggc acaaaaaatg ataaaagaaa atcttgatgc agatttagtt tttagtgatt
     1201 cttataattt aggagatgat aaatttatac taattacaac ttttgagaag tattatgcaa
     1261 gaaataatag tgatgcagga ttaatagtag tttatgaaaa taccacagga aaaacagtta
     1321 ttaaagctac ttcaacaggc agtgcaattg gaattttaaa aattgattgg ggtgcaggtg
     1381 gaaatttact taaaagagta aaaaacatac ttgctgatta tattatttaa ttattttatc
     1441 taactgataa tatgtcaaga aaatctcgta aaagaggtta agaacatttt tataatatat
     1501 agataaataa gaatttgaag gtaaagtttt taaggtataa agaaaaataa tataataaaa
     1561 cgaattggct ttataattct ttataaggca aagagcacat ttctttatat aattgttgga
     1621 tattaaataa tatgtaaata caccatttac atataattaa tataa
//
LOCUS       DAYAPA010000252         1591 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.257, whole genome shotgun sequence.
ACCESSION   DAYAPA010000252 DAYAPA010000000
VERSION     DAYAPA010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1591
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.257"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            139..1467
                     /locus_tag="ACGI59_003827"
     CDS             139..1467
                     /locus_tag="ACGI59_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFG9741554.1"
                     /translation="MGSNELKKTLGVSAALSTVVGSVIGAGVFFKPQAVYTLTGGAPG
                     LGILAWLIAGIITITAGLTAAEVSVAIPKTGGMMVYIKEIYGEKLGFLTGWMQIVLFY
                     PGMMAALGVIFGEQASALIGSPSLLLPIAIGIIIIVAGLNMLGSKTGGVIQTVSTICK
                     LIPLILIMVVGFIKGGGNNPILTPMVGEGLSLGSVLGQVLIAILFAFDGWMNVGTLAG
                     EMKNPGKDLPKAIIGGLSVVMAVYFIINLAYLWVLPANELANYASPASAVAEVIFGSM
                     GGKIISVGILISVFGALNGFLLTGSRVAYTLATDKTLPKYGIFSKLNSAQVPANAIAL
                     VSVIASIYALSGQFNLLTDLAVFATWIFYVLTFIGVMKLRKTHPNIPREYKVPLYPIV
                     PIIAIASGIFVVVNQLCFAGMKTTMISIGGLVITAIGLPVYAYMTRGVKR"
BASE COUNT          534 a          192 c          310 g          555 t
ORIGIN      
        1 aaaataaaag attactaaag ctaagtagag tttattttat tttaataaat aataaagcta
       61 tttttctata tattggcata atatatgctt atttaactat gaaattaaaa ttaaatagga
      121 ttaatgagga ggaaaaaaat gggaagtaat gaactgaaaa agactcttgg cgtatcagca
      181 gcattatcaa ctgttgttgg ttccgttata ggagcgggcg tattttttaa accacaagca
      241 gtctatacat taactggagg agctccaggt cttggtatat tggcatggtt aatagctgga
      301 atcataacta taacagctgg tcttacagct gcagaggtat cagttgcgat accaaaaact
      361 ggtggaatga tggtatatat aaaagaaatt tatggagaaa aattaggttt tttaactgga
      421 tggatgcaaa ttgtattatt ttatccaggg atgatggcag cattaggtgt tatatttgga
      481 gaacaagcat cggcactaat tggtagtcca tcacttttac ttccaatagc aattggaatt
      541 ataataatag ttgcagggct taatatgtta ggttcaaaaa ctggaggagt catacaaact
      601 gtttctacaa tttgtaagtt aataccactt attttaataa tggttgttgg atttattaaa
      661 ggtggtggaa ataatccaat acttacacct atggttggtg aaggattaag cctgggtagt
      721 gtactaggtc aagttttaat tgctatatta tttgcttttg atggatggat gaatgttggt
      781 actttagctg gagaaatgaa gaatcctgga aaagaccttc caaaagctat aataggtgga
      841 ttgtcagttg ttatggctgt ttattttata ataaatttag cgtatctttg ggtattacca
      901 gctaatgaat tagcaaatta tgcttctcct gcatcagcag ttgctgaagt tatatttggt
      961 tcaatgggtg gaaaaataat atctgtaggt atattaattt cagtgtttgg agcattaaat
     1021 ggatttttac taactggttc aagggtggca tatactttag ctacagataa gactttacca
     1081 aagtatggta tattttctaa attaaattct gctcaagttc cagcaaatgc aatagcatta
     1141 gtatctgtta ttgcatctat atatgctttg tcaggtcaat ttaatttatt aactgactta
     1201 gctgtatttg ctacttggat attctatgta ttaactttta taggggttat gaaacttaga
     1261 aagactcatc caaatatacc aagagagtat aaagttccat tatatcctat tgttcctata
     1321 atagctatag caagtggtat atttgttgta gttaatcaat tgtgttttgc aggtatgaaa
     1381 actactatga tatctatagg aggtcttgta ataacagcta taggcttacc agtgtatgct
     1441 tatatgacta gaggagtaaa aagataaacc aagtagttta caaatgcttc ataattaaaa
     1501 taaaagagac tgtctcaaat agagataagt ctctttttta ttgttaaaat ggtaaagata
     1561 tgtttaattt tacaaaaaaa gagcgttcca t
//
LOCUS       DAYAPA010000253         1544 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.258, whole genome shotgun sequence.
ACCESSION   DAYAPA010000253 DAYAPA010000000
VERSION     DAYAPA010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1544
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.258"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            74..1510
                     /locus_tag="ACGI59_003828"
     CDS             74..1510
                     /locus_tag="ACGI59_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp28"
                     /protein_id="HFG9741555.1"
                     /translation="MKFYKRILTLTLSISFVFANIQLVDAISSVEKIQGKNKYEIAGK
                     IADKNAYKTAILINTSNSIADGLSASGLAGALNAPILLTEKNTIPTETSARLKNVSKV
                     YIIGGTYSISTSVENSLKSKKMKVVRIKGNDRIKTSYNVAKEINSIKKVNTVMLTNAY
                     KGEADAISIASVAARDKAPIILTNGQSIPFSTSGLKSYVIGGTASMSTTLVNNTKSTR
                     LGGSTRFETNKAIINKFYKDAREFYIAGAYELTNALVGSSLSKHGPMVLVNDGSNKSI
                     LKNAKKITSIGYIDSNIVQQCLNITNGIGDINTGVVKNVKPTTKTIKDGMYKVGKDIS
                     AGEYLITSNSGSYASYYEVTSDSTGNADSILSNDIFSGTRYITLKNGQYIKIEDSTMT
                     LAKYAKAQKAKNGKFGNGMYKIGLEIPAGEYIIMSNSSDAYYEVRNDSLGNAEGIVTN
                     DTFSGRRYITVEEGQYLILNDCYLIENE"
BASE COUNT          632 a          162 c          266 g          484 t
ORIGIN      
        1 tgttaagtac ataactaatt taagaatatg tatttatata ttaaaaattt attatgaggg
       61 ggacaatttt attatgaaat tctataaaag aatattaaca ttgactttaa gtatttcttt
      121 tgtatttgca aatattcaat tagtagatgc aatatcatct gtagaaaaaa tacaaggaaa
      181 aaataagtat gaaatcgcag gtaaaatagc agataaaaat gcatataaaa ctgctatatt
      241 aattaatact agtaactcta ttgcagatgg attaagtgca agtggactag caggagcttt
      301 aaatgcccct atactattaa ctgaaaaaaa tactatacca actgagacat ctgcaagatt
      361 aaaaaatgta tcaaaagttt atataattgg aggaacatat tctattagta catctgtcga
      421 aaattcatta aaatctaaga aaatgaaagt tgtaagaatt aagggaaatg acagaataaa
      481 aacaagttat aatgttgcaa aagaaataaa ttcaataaaa aaagtcaata ctgttatgct
      541 aaccaatgca tataaaggag aagcagatgc tataagtatt gcatctgtag cagcaagaga
      601 taaagcacca ataattctta ctaatggaca aagtatacct ttttcaacta gtggtttaaa
      661 gtcttatgta ataggaggaa cagcttcaat gagtaccact ttagtaaaca atactaaatc
      721 tactagacta ggggggagta ctcgttttga aactaataaa gcaataataa ataagtttta
      781 taaggatgct agagagtttt atatagctgg agcttatgaa ttaacaaatg cacttgtagg
      841 atcttcttta tcaaaacatg gtccaatggt tttagttaat gatggtagca ataaaagcat
      901 tttaaaaaat gctaagaaga taacttctat aggatatata gattcaaata tagtacaaca
      961 gtgtttaaat ataacaaatg gaattggaga tattaatact ggggttgtaa aaaatgttaa
     1021 acctactaca aagactataa aagatggaat gtataaggtt ggaaaagata tctcagcagg
     1081 tgaatactta ataacttcaa actcaggaag ttatgcttct tattatgaag ttacaagtga
     1141 tagtacaggt aatgctgata gtatactttc aaatgatatt tttagtggga ctagatatat
     1201 caccttaaag aatggtcaat atattaaaat tgaggattca acaatgacat tagcaaagta
     1261 tgctaaagcc cagaaggcca agaatggtaa gtttggaaat ggtatgtata aaattggatt
     1321 agaaattcct gctggagaat atattataat gtcaaattct tctgatgcat attatgaagt
     1381 tagaaatgat agcttaggta atgcagaggg aatagttact aatgatactt tttcaggaag
     1441 aagatatata acggttgagg aaggtcaata tttaatatta aatgattgtt atttaattga
     1501 aaatgaatag atgtaattgt atttaatagt aaatcatata tttt
//
LOCUS       DAYAPA010000254         1542 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.259, whole genome shotgun sequence.
ACCESSION   DAYAPA010000254 DAYAPA010000000
VERSION     DAYAPA010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.259"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(334..825)
                     /locus_tag="ACGI59_003829"
     CDS             complement(334..825)
                     /locus_tag="ACGI59_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HFG9741556.1"
                     /translation="MGMIGCYTKISEENVFKLQQAEENLQDFVFEDANENSTINIDKA
                     WHAIHFTLTGCPFGGDDDNIFSKLVLSGNILMEIDGEFPVMLITANDVKKLSKAMNSL
                     EEQAFRKRFNINEMLENNIYPVMNDESEDDFFEYVWANLIELKKFIQEASDDGLAVIF
                     FIM"
BASE COUNT          585 a          200 c          132 g          625 t
ORIGIN      
        1 ttaatatata tttattttat ttgtgagtct atttttatat ataatgaaaa atgtgataag
       61 tatccaaaag aattactgga tataatagga tagggtattt agccttttaa gataaataaa
      121 aatatctaca actatatatt gcaaaaattt aaaactaatt tatatatgct aatataaata
      181 tttataaaga cataagacaa atttacctac agatttaaat ttaacttata tattttaaaa
      241 ggctaatata atatgtattt tcttctatac aatcaaaagg cttcatatct aagatatgtg
      301 atgacactaa tattcacaat tttttagtaa ttatcacatt ataaaaaata ttacagctag
      361 tccatcatca gatgcttctt gtataaactt tttaagctca attagatttg cccatacata
      421 ttcaaaaaaa tcatcttcac tctcatcatt cataacagga taaatattat tttctaacat
      481 ctcattaata ttaaatcttt ttctaaatgc ttgttcttcc aaactgttca ttgcttttga
      541 tagttttttt acatcatttg ctgtaatcaa catgacagga aattctccat caatttccat
      601 aagtatgttt ccacttagta ctaatttgct aaatatatta tcatcatctc caccaaaagg
      661 acaacctgtc aaagtaaagt gaatggcatg ccatgcttta tcaatattga tagtactatt
      721 ttcgttggca tcttcaaaaa caaaatcttg taagttttct tctgcttgct gtaatttaaa
      781 aacattctct tcacttattt tagtataaca tcctatcatc cccataatta cattcctcct
      841 aagtagcgtt tttttaattg taacttataa ttaaacttag ttacaataaa atatattgag
      901 aaaagtatta tttcatctta aatttaaata cctatattat caactaaaat tctgtgttta
      961 tagcgtaagt attattattt tttcctaaaa attaaatgaa tcagaagttc tataggcaat
     1021 attgactata aataagaact attactttta aaaaaaaatt taagaaatta tactgtagaa
     1081 tcatgctcta cttattagct ttaaataaaa aataaatcat gttctcaaat taaataataa
     1141 ataacaataa atttaaattt taaactataa gagtttctga agtgttaata agcataccaa
     1201 taattatatg tgtaagaatt tgatttaata gatatgaata atttttacat taattataaa
     1261 tatattaacc tattgttata aattctaaaa aagaaaagtt atcttaaata aaaaataagt
     1321 gtatcttaaa ttttaaatga tacacttatt tttatattga aattatattt tttaatatct
     1381 attcttaatt aggtattttt aaattatctg tttacattaa atatttctta attacttatc
     1441 tttcagctag ctttttctat taaatgtttc tcaattactt atttttcaat tagccactta
     1501 tattaagcat ttcttaatta cttatttttc aattagccac tt
//
LOCUS       DAYAPA010000255         1491 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.260, whole genome shotgun sequence.
ACCESSION   DAYAPA010000255 DAYAPA010000000
VERSION     DAYAPA010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1491
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.260"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            244..>1491
                     /locus_tag="ACGI59_003830"
     CDS             244..>1491
                     /locus_tag="ACGI59_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HFG9741557.1"
                     /translation="MNILGLIVEYNPFHNGHIYHLSKSIEKTNATHTVAIMSGNFLQR
                     GEPALFDKYTRAKIAVENGVDLVIELPTLFACQSAEIFSHGAITILNSLNCINSICFG
                     SEEGNTEILYNLAEILANEPNEFKLLLKKYLDDGLLFPTARANALFDYIHQDKKNNFI
                     QNYSKEQLSKTLNSSNNILGIEYIKNLIHLNSSIKPFTISRVQSEYNSEEIESDICSA
                     TAIRTSLKENTDLFHLKNVIPEKTYNILNEKIDNGFLPMFDDFFFDILKTIILRDMDT
                     LKEYFEVNEGIENRIYKDIFTSEFLHELQLAIKSKRYTLTKIKRTLNNILLGIKKSDV
                     QLIKNIDTIPYIRILAFNDKGREILKEIKNKSEIKIINKFSNISFSLDDTIFKTLISY
                     DIKATNIYNSIYYKNNKDLVKGPM"
BASE COUNT          633 a          182 c          169 g          507 t
ORIGIN      
        1 taataatata ttaacaagtt aacttaatgt agattattct ttcccatttg ttataataat
       61 ataatataaa gagctaataa agttatttta ataacattta accagttttt tatgtaggta
      121 acatttctac atttttataa atttttttac gttttttaat tttataatta aaaatttaaa
      181 ttttatacat attataataa attgtgatat atataaaata attaaagata aaaggagtat
      241 tttatgaaca tacttggatt aatagttgaa tataacccat ttcataatgg acacatatat
      301 catttatcta aatctataga aaaaacaaat gcaacacata ctgtagcaat aatgagtgga
      361 aattttctac aaagaggcga accagcttta tttgataaat acactagagc aaaaattgct
      421 gtcgaaaatg gtgtagactt agttatagaa ttaccaactt tatttgcttg tcaaagtgct
      481 gaaatttttt ctcatggtgc tataacaatt ttaaattcac taaactgcat aaattctata
      541 tgttttggta gtgaagaagg aaatacagaa attctttata atctagctga aattttagca
      601 aatgaaccaa atgaatttaa gttactttta aaaaaatatt tagatgatgg tctgttattt
      661 ccaacagcta gagctaatgc actttttgat tatatacatc aagacaaaaa aaataatttt
      721 atacaaaatt attcaaagga acaattatca aaaacattaa attcatcaaa caacatactg
      781 ggtatagaat acataaaaaa cctcatccat ttaaacagta gtataaaacc atttactatc
      841 tccagggtcc aatcagaata taattccgaa gaaattgaga gtgatatttg ttctgctact
      901 gctataagaa cttcacttaa agaaaataca gacctttttc atttaaaaaa tgttattcca
      961 gaaaaaactt ataacatcct aaatgaaaaa atagataatg gttttttacc tatgtttgat
     1021 gacttcttct ttgatatttt aaaaacaatt atacttagag atatggacac tttaaaagag
     1081 tattttgaag taaatgaagg catagaaaac agaatctata aagatatctt tacatctgaa
     1141 tttctacatg aactacaatt agcaattaaa tccaagcgat acactttaac caaaatcaaa
     1201 cgaactctaa acaatatatt gttaggtatt aaaaaaagcg acgtgcaact tataaaaaac
     1261 atagatacta taccttatat aagaatactt gcttttaatg ataaaggtag agaaattcta
     1321 aaagagataa aaaataaatc tgaaataaaa ataataaaca agttttcaaa tatatcattt
     1381 tcattagatg atacgatttt taaaacactc ataagttatg atataaaagc tacaaatata
     1441 tataattcaa tatattataa aaacaataaa gatttagtaa aaggccctat g
//
LOCUS       DAYAPA010000256         1459 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.261, whole genome shotgun sequence.
ACCESSION   DAYAPA010000256 DAYAPA010000000
VERSION     DAYAPA010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1459
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.261"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            684..1364
                     /locus_tag="ACGI59_003831"
     CDS             684..1364
                     /locus_tag="ACGI59_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFG9741558.1"
                     /translation="MQIDVLIIDGDKNSSQELKNFLEGKGINVYLAYNCKDAIIKIFS
                     KKFDLIFLEIILVDGDGWDFCKKIRNITTCPIIHITYINENQSILNALNSGGDDYLIK
                     PLNLEILYAKVKAILRRRDLFLNNNKNNIKLEDYNRASGVIKLENKMIKLTPTENRLL
                     NYFIENAEKTLTIKEIYEHVWMNEYLDDNYSVVVAVSGLRKKIEKDYKNPQKIITIRE
                     VGYYFNKI"
BASE COUNT          638 a           97 c          203 g          521 t
ORIGIN      
        1 ttatataaat caatacttaa acataccaat agaggcattt taaaaaaaac ataaatttat
       61 aatataaaat tgttttaagc tccctttccc caaaatagga aatttttatt agcatttaat
      121 aatgattttt aatttttaat ataatattaa agaattattt aagaatattt aatctctaaa
      181 gcttaaatta caataatatt ataaaaataa aagtgatata ttgtgtaata atcgaaattt
      241 tatactttgg ttaaaataat tttatatata gtgcctaatg tataacctaa tgctcgtaga
      301 caataatgtg gatattatca aatttatttt tataatagaa attttaattt atatctatat
      361 taaattgtta aagatataat tatgagaata aaaaatatag ctttgaatta tataattgta
      421 tatgcttatc tttatacagt aaataaaaaa tcaaatgaaa taccaaataa agtgtatatc
      481 ttaaaataac ttactagaga tacaaggatg ctatgtgaat tttgaagtgt gcagtttaga
      541 taataaatat atataaataa taaagaaaat ggatttatag tatttaaaat ttcttataag
      601 ggtaaaaaat gctattagaa aatgtactaa aaatattttt ccaattcttt atagaaaaat
      661 aatgatattt aggagagatt gtaatgcaaa ttgatgtatt gataattgat ggagataaga
      721 atagttctca agagctgaaa aattttttag aaggaaaggg gataaatgtc tatttagcat
      781 ataattgtaa agatgctatt ataaaaatat ttagcaagaa atttgactta atatttttag
      841 agataatttt agttgatggt gatggttggg atttttgtaa aaaaattaga aatataacta
      901 cttgcccaat tattcatata acttatataa atgaaaatca aagtatttta aatgctttaa
      961 attctggagg agatgattat ttaataaagc ctttaaattt agaaatatta tatgcgaaag
     1021 ttaaagctat tttaagaaga agagatttat ttttaaataa taataaaaat aatataaaac
     1081 tagaagatta taatagagct agtggagtaa taaagttaga aaataaaatg ataaagttaa
     1141 ctccaacaga aaatagatta ttaaattact ttatagagaa tgctgaaaag actctaacta
     1201 ttaaggagat atatgaacat gtatggatga atgagtattt agatgataat tatagtgtag
     1261 tggttgctgt gagtggatta agaaagaaaa tagagaaaga ttataaaaat cctcaaaaaa
     1321 ttataacgat aagagaagta gggtattact ttaacaagat ataaagcttt ataaacagaa
     1381 tgaatgataa ggcatagata atatgttagt atattataag agtttttgtt ctaacgatat
     1441 atttgtaaaa ttagattta
//
LOCUS       DAYAPA010000257         1420 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.262, whole genome shotgun sequence.
ACCESSION   DAYAPA010000257 DAYAPA010000000
VERSION     DAYAPA010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1420
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.262"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            206..427
                     /locus_tag="ACGI59_003832"
     CDS             206..427
                     /locus_tag="ACGI59_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat associated protein CotJA"
                     /protein_id="HFG9741559.1"
                     /translation="MGYLKEDKQDYCCEFARPYINRQIYDDTYNPKTALKRGTLFPEL
                     DLIESNNYNDWLYANPKNRVRAKKGGNMY"
     gene            427..690
                     /locus_tag="ACGI59_003833"
     CDS             427..690
                     /locus_tag="ACGI59_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein CotJB"
                     /protein_id="HFG9741560.1"
                     /translation="MSLNRRELLERISEYQFACIELNLYLDNNPRDKKALDSYNRYCD
                     KFTQAVCDYESKYGALTNFGYESSEYPWSWISEPWPWDKSFYK"
     gene            752..1324
                     /locus_tag="ACGI59_003834"
     CDS             752..1324
                     /locus_tag="ACGI59_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HFG9741561.1"
                     /translation="MWIYQKTLEHPVNIRQADPRMAKYIMTQLGGPNGELAAATRYLQ
                     QRYTMPTGKSRALLTDIGTEEMAHVEIISSVLYQLIGNCTPEELKAAGLGSNYANFGH
                     GLQPVDSNGVNFTTSYINVFGDSVTDLHEDMAAEQKALATYYQLINLTDDPDLKDILR
                     FLGEREVVHYQRFGEALMDVYEFTECKHQF"
BASE COUNT          559 a          166 c          249 g          446 t
ORIGIN      
        1 taaactctat attatttatt taaaatagat atttatgaaa gattaattag aaaaatttta
       61 gaggtaaaat aagaaaaaaa gatacttatt taatcattta atcttattaa agacctatta
      121 aaactaccag ttataatata gaaccagtat acaattttta aaaataatgc tattttaaaa
      181 ttctaaaaat taaggaggat aattaatggg atatttaaaa gaggataaac aagactattg
      241 ttgtgaattt gctagaccat acatcaatcg tcaaatttat gatgacacat acaatccaaa
      301 aacagcatta aaaagaggta ctttatttcc tgaattagat ttaatagagt caaataacta
      361 taatgattgg ctatatgcaa atcctaaaaa tagagttaga gctaaaaaag ggggtaacat
      421 gtactaatgt cattaaatag aagagaactt ttagagagaa tttcagaata tcaatttgcc
      481 tgtatagaat taaatttata tttagataac aatccaaggg ataagaaagc tcttgattca
      541 tacaacagat actgcgataa atttacacaa gctgtgtgtg attatgaatc taaatatgga
      601 gcattaacta attttggata tgaatcaagt gaatatcctt ggtcatggat atctgaacct
      661 tggccatggg acaaatcatt ttataaataa aattattaaa ctttaaaatt agatttatta
      721 ttaaatatat attaaggagg agtaagataa tatgtggatt tatcaaaaaa cactggaaca
      781 tccagttaac ataagacaag cagaccctag aatggcaaaa tatatcatga ctcagttggg
      841 aggacctaat ggtgagttgg cagctgcaac tagatatctt caacaaagat atactatgcc
      901 aactggaaaa tctcgtgcac ttttaactga tataggtaca gaggaaatgg ctcatgttga
      961 gataatttct tcagtgttat atcaattaat aggcaattgt actccagaag agcttaaggc
     1021 tgctggactt ggtagtaatt atgctaattt tggacatggt cttcagccag tagattctaa
     1081 tggagtaaac tttactacaa gttatattaa tgtctttggc gattcggtaa ctgatttaca
     1141 tgaggatatg gctgctgaac aaaaagcatt ggctacgtac tatcaattaa taaatttaac
     1201 tgatgaccct gatttgaaag atatattgag atttttgggt gagagggaag tagttcacta
     1261 tcaaagattt ggtgaagcat taatggatgt ttatgagttt acagagtgca agcatcagtt
     1321 ttaatgaaga attgaaagtg aatggagaat aagcatcagt tttaataaag aattaaaagt
     1381 gaacagagaa taagcatcat ttttaatgaa gaatcaaaag
//
LOCUS       DAYAPA010000258         1408 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.263, whole genome shotgun sequence.
ACCESSION   DAYAPA010000258 DAYAPA010000000
VERSION     DAYAPA010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1408
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.263"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            120..>1408
                     /locus_tag="ACGI59_003835"
     CDS             120..>1408
                     /locus_tag="ACGI59_003835"
                     /inference="COORDINATES: protein motif:HMM:NF020289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5906 domain-containing protein"
                     /protein_id="HFG9741562.1"
                     /translation="MSFDIFKGYIITNNKKAAEKFKNVKKLKTYEQIKNLPEFAGVLA
                     EETVLVDIDDFESSEILYKIVQDLKLKCRVYKTTRGKHFLFKNTSLEKNRTKCRLAIG
                     LNSDIKLGCKNSYSILKFNNIEREILYDSKEIQEIPMYLTPIKNGIDFLSLGEGDGRN
                     QALYNYILTLQSNDFTVEEIRETIRVINKYVLKTPLQDNELEVILRDESFQKKIFFNS
                     KGSFLFDEFAKYIKNNNHVIKINDQLHLYKDGIYVDGQARIEAEMINNISNLNKAKRS
                     EVLSYLNLLISENTSMSEANLIAFKNGIYNIVDDSFIEFSPEFIITNKVNWNYNPGAY
                     SKLVDKTMNKLSCGDFEIRMLLEEVVGYCFYRRNELRKAFILTGDKANGKSTYLDMIK
                     TLLGDENTSALDLKELSDRFKTAELFGKLANIGDDIG"
BASE COUNT          588 a          122 c          254 g          444 t
ORIGIN      
        1 aatggaagtg ttataggtaa aaaagtacca agaaagctag ataaaacttg gtatatagat
       61 ttagcaaatg aaagactcaa gcagtttggg gtattattaa gtaattgagg tggtgattgg
      121 tgagctttga catatttaaa ggatatataa ttacaaataa taaaaaggca gctgagaaat
      181 ttaaaaatgt aaaaaagtta aaaacttatg aacagattaa gaatttacca gagtttgcag
      241 gcgttttagc agaagaaact gtgttagttg atattgatga ttttgaaagt agtgaaattc
      301 tatacaaaat agtgcaagat ttaaagctaa aatgtagagt ttacaagaca acacgaggta
      361 agcattttct atttaaaaat actagtttag aaaagaatag aacaaaatgt agactagcta
      421 taggtttaaa ttcagatata aaattaggat gtaaaaattc ctattcaatt ttgaagttca
      481 acaatattga aagagaaatt ttatacgata gtaaagaaat acaagaaata ccaatgtatt
      541 taacaccgat taaaaacgga attgattttt taagtcttgg agaaggagat gggagaaatc
      601 aagcattata taattatatt ttaactctac agagtaatga ttttactgtt gaggaaataa
      661 gagaaacaat aagagttatt aataaatatg ttttaaaaac tccattacag gataatgaac
      721 ttgaagtaat tttgagagac gaatcctttc aaaagaagat attttttaat tcaaaaggtt
      781 catttttatt tgatgagttt gctaaataca taaaaaataa taatcatgta atcaagataa
      841 atgaccagtt acatttatat aaggatggta tctatgtaga tggtcaagca agaatagaag
      901 cagaaatgat taataatata agcaatttaa ataaggctaa gaggagtgag gtgcttagtt
      961 atttaaattt attaataagt gagaacacaa gtatgtcaga agctaattta atcgctttta
     1021 agaatggtat atataacata gttgatgatt cttttataga attttcacca gaatttatta
     1081 taacaaataa agttaactgg aactataatc caggagcata ttcaaagtta gttgataaaa
     1141 ctatgaataa attatcatgt ggagattttg aaattagaat gttacttgaa gaagtagtgg
     1201 gatactgttt ttatagacgt aatgagctta gaaaagcttt tatattaact ggagataaag
     1261 caaatggtaa atctacctat cttgatatga taaagacatt attaggggat gaaaatacat
     1321 cagcacttga tttaaaagaa ttaagtgata gatttaaaac tgctgaatta tttggaaaac
     1381 ttgcaaatat aggagatgat ataggaga
//
LOCUS       DAYAPA010000259         1337 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.264, whole genome shotgun sequence.
ACCESSION   DAYAPA010000259 DAYAPA010000000
VERSION     DAYAPA010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1337
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.264"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            27..101
                     /locus_tag="ACGI59_003836"
     tRNA            27..101
                     /locus_tag="ACGI59_003836"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:59..61,aa:Thr,seq:tgt)
     gene            116..200
                     /locus_tag="ACGI59_003837"
     tRNA            116..200
                     /locus_tag="ACGI59_003837"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:150..152,aa:Tyr,seq:gta)
     gene            210..293
                     /locus_tag="ACGI59_003838"
     tRNA            210..293
                     /locus_tag="ACGI59_003838"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:244..246,aa:Leu,seq:tag)
     gene            323..399
                     /locus_tag="ACGI59_003839"
     tRNA            323..399
                     /locus_tag="ACGI59_003839"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:357..359,aa:Arg,seq:tct)
     gene            407..482
                     /locus_tag="ACGI59_003840"
     tRNA            407..482
                     /locus_tag="ACGI59_003840"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:440..442,aa:Gln,seq:ttg)
     gene            571..659
                     /locus_tag="ACGI59_003841"
     tRNA            571..659
                     /locus_tag="ACGI59_003841"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:605..607,aa:Ser,seq:tga)
     gene            663..738
                     /locus_tag="ACGI59_003842"
     tRNA            663..738
                     /locus_tag="ACGI59_003842"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:696..698,aa:Phe,seq:gaa)
     gene            745..818
                     /locus_tag="ACGI59_003843"
     tRNA            745..818
                     /locus_tag="ACGI59_003843"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:779..781,aa:Met,seq:cat)
     gene            833..909
                     /locus_tag="ACGI59_003844"
     tRNA            833..909
                     /locus_tag="ACGI59_003844"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:867..869,aa:Ile,seq:cat)
     gene            933..1009
                     /locus_tag="ACGI59_003845"
     tRNA            933..1009
                     /locus_tag="ACGI59_003845"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:967..969,aa:Pro,seq:tgg)
     gene            1017..1093
                     /locus_tag="ACGI59_003846"
     tRNA            1017..1093
                     /locus_tag="ACGI59_003846"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1051..1053,aa:His,seq:gtg)
     gene            1102..1177
                     /locus_tag="ACGI59_003847"
     tRNA            1102..1177
                     /locus_tag="ACGI59_003847"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1135..1137,aa:Lys,seq:ttt)
     gene            1185..1258
                     /locus_tag="ACGI59_003848"
     tRNA            1185..1258
                     /locus_tag="ACGI59_003848"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1217..1219,aa:Cys,seq:gca)
     gene            1265..1336
                     /locus_tag="ACGI59_003849"
     tRNA            1265..1336
                     /locus_tag="ACGI59_003849"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1297..1299,aa:Asn,seq:gtt)
BASE COUNT          321 a          309 c          355 g          352 t
ORIGIN      
        1 ccccttccag gtcgccaaaa taaaatgctg gtgtggctca acggtagagc agctgacttg
       61 taatcagcag gttgtaggtt cgattcctat caccagctcc atgtaaaatt aatacggagg
      121 atttcccgag cggccaaagg gggcagactg taaatctgtt gtcatcgact tcggtggttc
      181 gaatccacca tcctccacca aaaaaatgcg cggatgtggc ggaattggca gacgcactag
      241 acttaggatc tagcgccatt ggcgtggggg ttcgactccc ttcatccgca ccatttttgt
      301 ttgatataca tatataaatt gtgggtgcat agctcagctg gatagagcaa cgcccttcta
      361 aggcgtgtgt ccggggttcg aatccctgtg cgctcaccac atatattggg gattagccaa
      421 gtcggtaagg cacatgactt tgactcatgt atgcgtaggt tcgagtcctg catccccagc
      481 caaatgatgt attactaagc tatatttgaa ttatttttat tttaaatata gttaggttca
      541 attttagaat aagtttaagt atccgaatgt ggagaggtgt ccgagtggtt taaggagctg
      601 gtcttgaaaa ccagtgactt cgaaaggggc cgtgggttcg aatcccaccc tctccgccaa
      661 atgcccagat agctcagtcg gtagagcagg ggactgaaaa tccccgtgtc ggtggttcga
      721 ttccgcctct gggcaccaaa atatggcggt atagcttagt tggctagagc gttcggttca
      781 tacccgaaag gtcataggtt cgactcctat taccgctact aaaatttaat gtgggccatt
      841 agctcagttg gttagagcgc ccggctcata accggtaggt ctggggttcg agtccctgat
      901 ggcccaccac attaaattaa ttaatataaa ttcgaggtgt agcgcagttt ggtagcgcac
      961 atggtttggg accatggggc cgggggttcg agtcccttca cctcgaccaa tataaggtgg
     1021 gtatagctca gttggttaga gcgccagatt gtggctctgg aggtcgtgag ttcgactctc
     1081 attatccacc ccaattcata tggtctatta gctcagtcgg tagagcacct gacttttaat
     1141 cagggtgtcc cgcgttcgag tcgcggatag atcaccaaaa aggtggcgac atagccaagt
     1201 ggtaaggcag tggactgcaa ctccttgatc cccagttcga atctgggtgt cgcctccaaa
     1261 atattccaag gtagctcaac ggtggagcaa ccggctgtta accggtaggc tgtgggttcg
     1321 agccccaccc ttggagc
//
LOCUS       DAYAPA010000260         1286 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.265, whole genome shotgun sequence.
ACCESSION   DAYAPA010000260 DAYAPA010000000
VERSION     DAYAPA010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1286
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.265"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            185..1225
                     /locus_tag="ACGI59_003850"
     CDS             185..1225
                     /locus_tag="ACGI59_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFG9741563.1"
                     /translation="MDKHSDSVMYGGMEVYNIWGTTTVDSIIGESDVMRALKNRICKI
                     GNSDSTVAITGESGTGKELIARAIHLAGNRGNKTFVAINCAAIPEPLLESELFGYTKG
                     AFSGADSKGKIGKFELANGGVVFLDEIGDMPIQLQSKLLRVLQEKKFTRIGSNELIDI
                     DVRIISATNRDLFELVKENKFREDLYYRLNVIPLEVPPLRERGDDIQILINNFIDKYS
                     RKMKKNVYHIEPDVEQMLLKYPWPGNIRELENTIELALNLLEEDGIIHKGIINRKILE
                     YFKLNNINIKLLEESDYEISMEQDILTLEEFERAYIKKVLKFYGNDTKSKKLAAKKLG
                     ISLATLYRKIDV"
BASE COUNT          521 a          129 c          243 g          393 t
ORIGIN      
        1 tagaggattt ttgtaaaatg aagaaagtat atctatcaaa ttaaaactaa gtagatatac
       61 tttttattaa gttttgtttt aaaataatat tattaaatta taactaagct tttctgttat
      121 gatataatta aatagataat gtgaatgatt attatataga atacttattt ggagttgata
      181 ttttatggac aaacacagtg attcagtaat gtatggtgga atggaagtat ataatatttg
      241 gggaacaaca acagtagact ccataattgg agagtctgat gtaatgcgtg ctcttaagaa
      301 tagaatttgt aaaattggaa atagtgattc tactgtagct ataactggag aaagtggcac
      361 tggaaaagaa ttaatagcaa gagccattca tctcgcagga aacagaggga ataaaacatt
      421 cgttgctata aactgtgctg ctataccaga acctttacta gaaagtgaac tttttggata
      481 tacaaaagga gcttttagtg gagcagattc aaaaggtaaa attggtaagt ttgaattggc
      541 caatggaggg gttgtatttt tagatgagat aggagatatg ccaattcaac tacaatcaaa
      601 gttattgagg gtattacagg aaaagaagtt tacgagaata ggttcaaatg aactaattga
      661 tatagatgtt agaatcatat cagctactaa tagagattta tttgagttgg ttaaggaaaa
      721 taaatttaga gaagacttat actatagatt gaatgtaata cctttagaag tgcctccact
      781 tagagaaaga ggagacgata tacaaatatt aataaacaat tttatagaca aatattcgag
      841 aaagatgaag aaaaacgtgt accatataga gccagatgtg gaacaaatgt tgcttaagta
      901 tccttggcca ggaaatatta gagagttgga aaatactata gaactggcat taaaccttct
      961 tgaggaagat ggaataatac ataaaggaat aataaataga aagatacttg aatattttaa
     1021 gttaaataat ataaatataa agttacttga ggaaagtgac tatgagataa gtatggaaca
     1081 agacatactt acacttgaag agtttgaaag agcctatata aagaaagtgt taaagtttta
     1141 cggaaatgat actaagtcta aaaaactagc tgccaaaaaa cttggaatat cactagctac
     1201 attgtacaga aaaatagatg tataataatt aggcttttta ttctcaattc taaaatttga
     1261 aattataaaa ttgataaaaa acactt
//
LOCUS       DAYAPA010000261         1269 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.266, whole genome shotgun sequence.
ACCESSION   DAYAPA010000261 DAYAPA010000000
VERSION     DAYAPA010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1269
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.266"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            180..890
                     /gene="rgaR"
                     /locus_tag="ACGI59_003851"
     CDS             180..890
                     /gene="rgaR"
                     /locus_tag="ACGI59_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RgaR"
                     /protein_id="HFG9741564.1"
                     /translation="MFRVVICDDEKIQRSILKEFTEKLLSERCLNYQILEFSCGEDLI
                     SKYPEKIDIIFLDIQMKDINGIEVAKKIRKFDEKVEIIFTTAFSEYAPQGYEVRAYRY
                     LVKPIEYSNFAMGVNLCIDNLQRKKERYIVLNSKKGFNRILIDSILYVETVKRDLVVH
                     TIGRDYKANMSMKQAEKLLCENGFFRCHTSFIVNLRRIEDLRDNMITINEQFIPISKY
                     RLKDLKVALTSLLCDIMY"
BASE COUNT          506 a          103 c          204 g          456 t
ORIGIN      
        1 tatctagaaa agtataggaa tttatcgtaa tttaagttta aatattaaag agaatttcta
       61 taagtagttt aatcgacaat ttaaatttat aaagaaattt aaatttgaaa gctagtgagt
      121 acagtttgga aattgaaata aagtaaacta aatgatattt tagatttgga ggtgatatta
      181 tgttcagggt agttatatgt gatgacgaaa aaatacaaag aagcatacta aaagagttta
      241 cagaaaaact tttgtctgaa agatgtctaa attatcaaat actggaattt tcctgtggag
      301 aagatttaat atccaaatac ccagaaaaaa ttgatattat atttttagat atccaaatga
      361 aagatatcaa tggaattgag gtggctaaaa aaataagaaa gtttgatgaa aaagtagaaa
      421 ttatatttac aacggctttt tcagagtatg ccccccaagg ttatgaggtt cgtgcttata
      481 gatatttagt aaagcctata gagtatagta actttgcaat gggtgtaaat ctttgtattg
      541 ataaccttca aagaaagaag gagcgttata tagttttaaa tagtaaaaaa ggatttaata
      601 gaatactgat agattctatt ttatatgttg aaactgtaaa gagagattta gtcgtacata
      661 caataggtag agattataaa gctaatatga gcatgaaaca agcagaaaaa ttactgtgtg
      721 aaaatggctt ttttagatgt catacaagtt ttattgttaa tttaagaaga atagaagatc
      781 ttagggataa tatgattact attaatgaac aatttatacc tataagtaag tatagactta
      841 aagatttaaa agtagcatta acaagcttgt tatgtgatat aatgtattaa ttttaaaaag
      901 gtaattgaaa atatcgtaaa aaagatttaa tgtcatcaca gggctattga ttgaaactgt
      961 cttaatatat aaaatgttat ttatattaag acagttttat tatgcaaaat agcagtttat
     1021 agatttttaa tatggtttaa catgtatatg tagaggttcc attatagtag catgattatt
     1081 aattagtagc ttgattatca atatactatt attgattttt atatgcatta tatgtatttt
     1141 tagaatatga ttaccttttt aaaataatga ttatttataa aactctatag atatttgttt
     1201 catttagcta cgcatacttt aattaattat attaatattt aatggagtac aatataaata
     1261 ttaaagcga
//
LOCUS       DAYAPA010000262         1260 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.267, whole genome shotgun sequence.
ACCESSION   DAYAPA010000262 DAYAPA010000000
VERSION     DAYAPA010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1260
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.267"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(<1..896)
                     /locus_tag="ACGI59_003852"
     CDS             complement(<1..896)
                     /locus_tag="ACGI59_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HFG9741565.1"
                     /translation="MRNIILYLNDDTFISKKYPDKNFSNLDYCLIGSKCSNSFVKEKL
                     ITFFKVRIPDILKDKSILKAELFIHIDSNKNHIFKEKVDIEIKRISEYYNLRTITWND
                     RVSMENIRGYLPVGISDTSNYICLNITGTIKAWAMNKYPNYGLALSLNYPYQILEFTS
                     SRGCNKPYILVTFEDRIIDNCYPKCECLPIRITGPMGPRGATGSTGPTGVTGPTGSTG
                     ATGSIGPTGPTGNTGATGSIGPTGVTGPTGSTGATGSIGPTGVTGPTGNTGEIGPTGA
                     TGPTGVTGSIGPTGATGPTGEIG"
BASE COUNT          362 a          230 c          153 g          515 t
ORIGIN      
        1 cctatttctc ctgttggtcc tgttgctcct gttggtccta tacttcctgt cactcctgtt
       61 ggacctgttg ctcccgttgg tcctatttct cctgtatttc ctgttgggcc tgttactcct
      121 gttggtccta tacttcctgt cgctcctgta cttccggttg ggcctgttac tcccgttggc
      181 cctatacttc ctgttgctcc tgtatttccg gttgggcctg ttggtcctat gcttcctgtc
      241 gctcctgtac ttccggttgg gcctgttact cccgttggtc ctgtacttcc tgtcgctcct
      301 cttggtccca ttggacctgt aattctaatt ggaagacact cacatttagg ataacaatta
      361 tctataattc tatcttcaaa tgttacaagt atatacggtt tgttacaacc tctactagat
      421 gtaaattcaa gaatctgata agggtaattt aaagataaag ctaacccata attaggatat
      481 ttattcattg cccatgcttt tatagttccc gtaatattta aacaaatata gttggatgta
      541 tcacttatcc caactggtaa atatccccta atattttcca tagacactct atcattccat
      601 gttatagttc gtaaattata atattcactt attcttttaa tttcaatatc tactttttct
      661 ttaaaaatat gattcttatt tgaatcaata tgaataaata actctgcttt taatatactt
      721 ttgtctttta atatatctgg tattctcact ttaaaaaaag taatcaactt ttcttttaca
      781 aaactatttg aacatttact tcctattaag caataatcta aattactaaa gtttttatct
      841 ggatattttt tagatataaa agtatcatca tttaaataaa gtataatatt tctcatttat
      901 aatccctcca ttttgggtct atatcatact atgtttaaaa tacatgctta gttacaatat
      961 aattaaaatt tatagtatta aatttttata taataatgct gatttctaca aaaaaagaac
     1021 atgtcattag aaatttctaa tagcatgttc tttttcatta ttatatatta cactttaaat
     1081 tattccttga ctggcttagc tggtgtaaac taaaccagtc tttctaaaat attaagcact
     1141 tttttcttat tatgtttttt aggatttatt gtctatatta ttgtaaacca aagttataaa
     1201 attgtaaata gaatatatta ccataacaat atctttttat aagtaaaaat tataaaaaac
//
LOCUS       DAYAPA010000263         1203 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.268, whole genome shotgun sequence.
ACCESSION   DAYAPA010000263 DAYAPA010000000
VERSION     DAYAPA010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1203
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.268"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            91..1131
                     /locus_tag="ACGI59_003853"
     CDS             91..1131
                     /locus_tag="ACGI59_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HFG9741566.1"
                     /translation="MNLNLTNTINLMKKYLDIPSPAGYTENAVLEIKKDFENLSLDTI
                     LTKKGALIATLPGEDDSNQITISAHMDTLGAIVKEISSDGTLKYHKVGGGCWAAIEGE
                     NCTVITRKGKKIRGSVVFKYASTHIYGQTKAATERNEETMIIRLDEKVFTKQDVLDLG
                     ISVGDFVCLDHRFEATESGFIKSRYIDNKSAVAMVLEICRYFKENNLTPKHTTNFYIS
                     NYEEIGHGVSKCLPEKTKEFVAIDIAPTGPSQTSSEHGVTIVAKDNLSLYDFNLRNKL
                     VDVAEDNNLDYAVDVFTFYGSDASEAIRWGEDVQIACVGPGTNNSHHYERTHIEAIEN
                     TLKLLINYMLKE"
BASE COUNT          467 a          142 c          195 g          399 t
ORIGIN      
        1 gtttagattc tattctttta attttagttt tcagaacatt tgtatttatg ttataatttg
       61 tataaatata tttatttagg aggaaacaat atgaatttaa atttaactaa tactataaat
      121 ttaatgaaaa aatatttgga cattccaagc ccagctggat atactgaaaa tgcagtttta
      181 gaaatcaaaa aagattttga aaatttaagt cttgacacta ttcttacaaa aaaaggtgca
      241 cttatagcta ctcttcctgg tgaagatgat tcaaatcaaa taacaatatc tgcacatatg
      301 gatacacttg gagctatagt aaaagaaata tcatctgatg gtactcttaa ataccataaa
      361 gttggtggtg gatgttgggc ggctatagag ggagaaaact gtactgttat aactagaaaa
      421 ggtaaaaaaa tcagaggaag tgttgtattt aagtatgctt ctactcatat atatggtcaa
      481 acaaaagctg ccactgaaag aaatgaagaa acaatgatta ttagacttga tgaaaaagta
      541 tttacaaagc aagatgtatt agatttagga ataagtgttg gggattttgt atgcctagac
      601 catagatttg aggctacaga atctggtttt ataaaatcta gatatataga taataaatca
      661 gctgtagcaa tggttcttga aatatgtaga tattttaaag aaaacaattt aacacctaag
      721 catactacta acttttatat aagtaattat gaagaaattg gacatggtgt ttctaaatgt
      781 ttacctgaaa aaacaaaaga atttgtagca atagatatag cacctactgg accaagtcaa
      841 acatctagtg aacatggtgt aactatagtt gcaaaagata atttatcatt atatgatttt
      901 aatttaagaa acaagcttgt cgatgttgca gaagacaata atttagacta cgctgtcgat
      961 gtatttactt tctatggttc agatgcttca gaagctattc gttggggaga agatgttcaa
     1021 atagcatgtg ttggtcctgg aacaaacaat agtcaccatt atgaaagaac acatatagaa
     1081 gctattgaaa atacactaaa attacttata aattatatgc ttaaagaata attaattata
     1141 tattttacca aagtcaattc ttaacaaata attaacttta atttttattt ttatacaaaa
     1201 att
//
LOCUS       DAYAPA010000264         1193 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.269, whole genome shotgun sequence.
ACCESSION   DAYAPA010000264 DAYAPA010000000
VERSION     DAYAPA010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.269"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            <1..1170
                     /locus_tag="ACGI59_003854"
     CDS             <1..1170
                     /locus_tag="ACGI59_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFG9741567.1"
                     /translation="IINTTFSESMENVKNKVIVVDQYGSKISEKIDNEIFKEVNVIMQ
                     KVIQQQENQDVDIDSEFNGIEKSCSLKGYGDVSCITGRGVKVKDSYTKLVGLFYIDTD
                     KHTWQNGEYQIELELNFQNLMDEKSAGQDEPKEESNLGGEDYAGGKEFTAEFTAYCPR
                     KEEGGDTDCRKKKLDPSKKTCAAPMVGKYEQTYYTKEFLNKHPLLNYGDEIQVITGVS
                     GRDGVYKVNDVGPAITIEKNGTYHIDILFGNVEEASKFGRRKGKIIIGGYSGNVSDKA
                     KIVISEAKKHLGKPYKWGGNGPSSFDCSGLMVYCFKKVNVSLPRTSNQQSKKGKKVEQ
                     KNLQAGDLVFFHNPVSHVGLYIGNGEFLHAPQKGDVVKISKLSSRRDFNTARRVL"
BASE COUNT          491 a          121 c          249 g          332 t
ORIGIN      
        1 attataaata ccaccttttc ggagagcatg gaaaatgtaa aaaataaagt aatagtggta
       61 gaccagtatg gaagcaagat tagcgaaaaa atagataatg aaatttttaa ggaagtaaat
      121 gtaataatgc aaaaagtaat tcagcaacaa gaaaatcaag atgtagatat tgatagcgag
      181 tttaatggga tagaaaaaag ctgttctctt aaaggttatg gagatgtaag ttgtataact
      241 ggtagaggag taaaagttaa agattcttat acaaagcttg taggactatt ttatatagat
      301 acagacaaac atacttggca aaatggagaa tatcaaattg agcttgaact taattttcaa
      361 aatcttatgg atgaaaagtc agcaggacag gatgaaccta aggaagaaag taacttaggg
      421 ggagaagatt atgcaggagg aaaagagttt acagcagaat ttacagctta ctgtcctaga
      481 aaagaagaag gtggagatac agattgtaga aagaaaaaac ttgacccatc taaaaaaact
      541 tgcgctgctc ctatggttgg taaatatgaa caaacttatt atacaaaaga gtttttaaat
      601 aaacatcctt tattaaacta tggagatgaa atacaggtaa ttacaggagt ttctggtcgt
      661 gatggagtct ataaagtaaa tgacgtagga cctgcaataa ctatagaaaa gaatggaaca
      721 taccatatag atattttatt tggaaatgtt gaagaagcta gtaaatttgg aagaagaaaa
      781 ggaaaaatta ttattggtgg ttattctggt aatgtatctg ataaagctaa aatagtaata
      841 tcagaagcaa aaaaacatct aggtaaacct tataaatggg gtggaaatgg accaagtagt
      901 tttgactgtt ctggtttaat ggtctattgt tttaaaaaag ttaatgttag tttgccaaga
      961 acgtcaaatc aacaatctaa aaaaggcaag aaagtagaac aaaaaaatct tcaagcagga
     1021 gatttagtat tttttcataa tccagtcagc catgttggat tatatatagg taatggagaa
     1081 tttttacatg ctccacaaaa aggtgatgta gttaaaataa gtaagttaag tagtagaaga
     1141 gattttaata cagctaggag agtattataa aaggatggtg atataatggc taa
//
LOCUS       DAYAPA010000265         1153 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.270, whole genome shotgun sequence.
ACCESSION   DAYAPA010000265 DAYAPA010000000
VERSION     DAYAPA010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1153
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.270"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            <1..>1153
                     /locus_tag="ACGI59_003855"
     CDS             <1..>1153
                     /locus_tag="ACGI59_003855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9741568.1"
                     /translation="DVFIERKSEFEAHMGLIKAFKLPVAYIGKTQTQLTAIILEATKK
                     EHDDEFDLQIPDTLKIEKYKEVLSWYKNPLNHDYSKNLKINISNVPHVFAWGGVHGAI
                     TKYYGEGYFLHVDVNSFYPSLMIRYNYHSRNIKNPQKYVEIYDKNLQLKKEKSPLRPA
                     YKLAVNKTYGGMKDKNNNLYDPRQANNVCVSGQLLLLDLIEKLEGHCKLIQSNTDGLI
                     VKLNTIDDYELIDDICYEWEQRTGMGLGFDVYTKIFQKDVNNYLVITEDGEVEAKGLY
                     IKDLGNLDYDLPIINKALKNYMVNNTPIEETINKCNDLIEFQKIVKISSKYSHGLYSP
                     TIEGKTKKVFIGGKILNDKCFRVFASKNKNDGGIYKVKNKEKNPEKFANT"
BASE COUNT          492 a          124 c          189 g          348 t
ORIGIN      
        1 tagatgtttt tattgaaaga aaatcagaat ttgaagcaca tatgggatta attaaagctt
       61 ttaaacttcc agtagcatat attggtaaaa ctcaaacaca actaacagca attatattag
      121 aagctacaaa gaaagaacat gatgatgaat ttgacttaca aataccagat actctaaaaa
      181 ttgagaaata taaggaagtt ttaagctggt ataagaaccc actaaatcat gactattcta
      241 aaaatctaaa aattaatatt tctaatgtac ctcatgtatt cgcttggggt ggtgttcatg
      301 gagcaataac aaaatactat ggtgaaggtt attttttaca tgtggatgtt aattccttct
      361 atccaagtct tatgattaga tacaattacc actcaagaaa tataaaaaat cctcaaaagt
      421 atgttgaaat ttatgacaaa aacttacagt taaaaaaaga aaaatcacct ctaagaccag
      481 cctacaaatt ggcagttaat aaaacttatg gaggaatgaa agataaaaac aataatttat
      541 atgacccaag acaggctaat aatgtatgtg ttagtgggca gctgttatta cttgatttaa
      601 tagagaagtt agaaggtcat tgtaaactta ttcagagcaa tacagatgga ttaattgtaa
      661 agttaaatac aatagatgat tacgaattaa tagatgatat atgttatgag tgggaacaaa
      721 ggacaggtat gggtttaggt tttgatgtat acactaaaat atttcaaaag gatgttaaca
      781 attatttagt tataacagag gatggagaag ttgaagcaaa aggattatat atcaaagatt
      841 taggaaatct agactatgat ttaccaatta taaataaagc acttaaaaat tatatggtaa
      901 acaatacacc tattgaagaa actataaata aatgtaatga tttgatagag tttcaaaaga
      961 tagtaaaaat aagtagtaaa tattcacatg gtttatatag tccaactata gagggaaaaa
     1021 caaaaaaagt atttattggc ggtaagattt taaatgataa atgttttaga gtatttgctt
     1081 caaaaaataa aaatgatggt ggtatttata aagtcaaaaa caaagaaaaa aatccagaaa
     1141 aatttgctaa tac
//
LOCUS       DAYAPA010000266         1143 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.271, whole genome shotgun sequence.
ACCESSION   DAYAPA010000266 DAYAPA010000000
VERSION     DAYAPA010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1143
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.271"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            <1..815
                     /locus_tag="ACGI59_003856"
     CDS             <1..815
                     /locus_tag="ACGI59_003856"
                     /inference="COORDINATES: protein motif:HMM:NF042696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFG9741569.1"
                     /translation="LMNKIINNILNTCKQYTYILPHKLLRQYIAHYTISIPDTSIKEN
                     LTLIPDASGCMIFKFDKKGIESAFWGATTKTTIVKNDIENVLFRVFVEFRPGGVYYLT
                     GLSQRESTDLKISLEDFNTLFSLEVNSIFERTSTIKELVEQLDMLFLSYLLKSNIVDM
                     TIPILENARKQNSIMSVKNISQISCYSERHLNRIFNNSLGMSVKSYLRLLRINLVLQE
                     IQNNKIPFATLAQDIGYYDQSHFINDFKSICGVNPTTYIKNLSDFYNEKYKF"
     gene            857..1123
                     /locus_tag="ACGI59_003857"
     CDS             857..1123
                     /locus_tag="ACGI59_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HFG9741570.1"
                     /translation="MIESRCGILCYECGYKEKVNCKGCTEIEKPFWGEQCPVKSCCED
                     KNLTHCGLCDTFPCEMLNQFAYDKEQGDDGKRISQCKKWAVLAL"
BASE COUNT          447 a          143 c          168 g          385 t
ORIGIN      
        1 tacttatgaa taaaataatc aataacattt taaatacctg caaacagtat acatatattt
       61 tgccacataa attgttaaga cagtatatag cacactatac aatatctatt ccagatacaa
      121 gtattaaaga aaatcttaca ttaatacctg atgctagtgg atgtatgata tttaaatttg
      181 acaaaaaagg tatagaaagc gcattctggg gagccacaac aaaaactact attgtaaaaa
      241 atgatataga aaatgtactt tttagagtat tcgttgagtt tcgcccaggt ggagtatatt
      301 atttgacagg tctttctcaa agagaatcta cagatttaaa aatttcactt gaagatttta
      361 acacattatt tagcttagag gttaattcca tttttgaaag aacaagtact ataaaggaat
      421 tagtagaaca attagatatg ttattcttat catacttatt gaaatcaaat atagtagata
      481 tgactatacc aattcttgaa aatgctagaa aacaaaactc tattatgtct gttaaaaaca
      541 tttcacaaat tagttgttat agtgaacgac atttaaatag aatttttaat aattctcttg
      601 gtatgagtgt taaatcatac cttagattac ttcgtataaa ccttgtatta caagaaatac
      661 aaaataataa aataccattt gcaactttag cacaagatat tggttattac gaccaatctc
      721 attttataaa tgattttaaa tctatttgtg gtgttaatcc aacaacctat ataaaaaatt
      781 tgtccgattt ttacaatgaa aagtataaat tttgatatac aatcatataa aatttatatt
      841 taaggagtga cttaaaatga tagaatcaag atgtggaatt ttatgttatg aatgtggata
      901 caaggaaaaa gtcaattgta aaggttgtac agagattgag aaaccatttt ggggtgaaca
      961 atgtcctgta aaatcttgtt gtgaagataa aaaccttaca cattgtggtc tatgtgatac
     1021 atttccatgt gaaatgctta atcaatttgc atatgacaaa gaacaaggtg atgatggcaa
     1081 aagaatttct cagtgcaaaa aatgggctgt actagcctta taatatactc tcttgttata
     1141 aaa
//
LOCUS       DAYAPA010000267         1083 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.272, whole genome shotgun sequence.
ACCESSION   DAYAPA010000267 DAYAPA010000000
VERSION     DAYAPA010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1083
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.272"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            428..895
                     /locus_tag="ACGI59_003858"
     CDS             428..895
                     /locus_tag="ACGI59_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFG9741571.1"
                     /translation="MKFLNVLVVVEDIEKSKKFYYDVLGLKVICDFGENVVLEGNISL
                     QEKKLWLEFINKSDSEVKFNGNDAELYFEEDNFDTFVERLSTMKDIDYVHLAIEHRWG
                     QRAIRFYDLDGHIIEVGETMSSVCRRFLDSGLSIDEVAKRMDVTVEYIESVLE"
BASE COUNT          425 a           71 c          171 g          416 t
ORIGIN      
        1 attagaaaga ttatgaaaac taaattagaa gaactaaaaa ctatattttt attaattata
       61 atatttgaat aatcttttta atcatataaa atgcatatac ttgtaatatt taaaggtgat
      121 attaatatta ttagtgtata ttaatattcc cttgaaatta aaaaaacata gatatttcag
      181 taatctaaaa tagtctacaa aaataacagt tttagcaaaa ttaataagaa tacatataga
      241 tttaaaccta aaagtatgaa catgtctgtt ttttacaaga ttaaaattta tattatatga
      301 taaaattaac ttaactattt caataagact tataattttt ttctttatac tagaccttat
      361 tgaaaccttt atagtttaat tctaattata tattataaat aaagtatgtg tatattaagg
      421 aggtaatatg aagtttttaa atgttttagt agtagttgaa gatattgaga aatcaaaaaa
      481 gttttattat gatgttttgg ggttaaaggt aatctgtgat tttggagaaa atgttgtatt
      541 agaaggtaat atatcattac aagaaaaaaa gctttggtta gaatttatta ataagagtga
      601 cagtgaagtt aagttcaatg gaaatgatgc tgagttatat tttgaggaag ataattttga
      661 tacttttgtt gaaagattga gtacaatgaa agatatagat tatgttcatc tagctataga
      721 gcataggtgg ggtcaaagag caattagatt ttatgattta gatgggcata ttattgaggt
      781 tggagaaact atgtcatctg tgtgtagacg ttttttagat agtggtcttt caatagatga
      841 ggtagctaaa agaatggatg taactgtcga atatattgaa tcagttttag aatagatgga
      901 ttatattaaa gtaaaattga ggtgaaggct gtaagtaaac ttgtgtgaaa acaaatttgc
      961 ttacagtttt tatttttcta aaataaagaa tataatactt gtttagcttt atttgagttt
     1021 ttaaaaatat tatgatattt cttatatatt tatactaaag tttaaattta gaaaaaatat
     1081 ata
//
LOCUS       DAYAPA010000268         1082 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.273, whole genome shotgun sequence.
ACCESSION   DAYAPA010000268 DAYAPA010000000
VERSION     DAYAPA010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1082
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.273"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(141..926)
                     /locus_tag="ACGI59_003859"
     CDS             complement(141..926)
                     /locus_tag="ACGI59_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFG9741572.1"
                     /translation="MNFENDFITPEFRKKYIGLVLDNAQNINKVYTTTFPDKEIIKQV
                     IDKDEKDILIFSHHAMGYIASDEGFPFHDIPLSYMEEMKNRRISFYVLHSPLDNYSDY
                     STSVSFAKAMGLEIVKPFCKYDDKIEVGVICKTSLKSIEEIKELIKKSVGHDVKLYDY
                     GDSVLKDGLVAIAAGGGSYPFVAREVAELGINLYITGFTKPLKHFEPVLEFHQIAKDN
                     LINVIGATHYSTEKFACMGMVDYFKNLGLESEFLQGKYYLEDL"
BASE COUNT          392 a          167 c          107 g          416 t
ORIGIN      
        1 taatcagcaa ttaaaagtct attttcttag tttaaaaaat aaagatagat tttttcaaaa
       61 cactggataa ataggtaaaa aaataaacag ttagtaaaat aaatttttac taactgttta
      121 tttttattgt ttaatatgtc ctataaatcc tctaagtaat attttccttg caaaaattct
      181 gattctaaac ctagattttt aaaataatca accatgccca tacacgcaaa tttttctgta
      241 gaataatgag tagcacctat aacattaatt aaattatctt ttgctatttg atggaactct
      301 aatactggtt caaaatgttt taaaggctta gtaaacccag taatatatag atttatacca
      361 agttcagcaa cttctcttgc tacaaatgga taacttcctc cccctgctgc aatagcaact
      421 aaaccatctt ttaatacaga atctccataa tcatacaatt tcacatcatg accaacagat
      481 tttttaatta attccttaat ctcttctatt gattttagtg atgttttaca tataactcct
      541 acctcaattt tatcgtcata tttacaaaat ggtttgacta tttctaaacc catagctttt
      601 gcaaaactaa cagaagttga atagtcacta tagttgtcta gtggagaatg taatacataa
      661 aaagaaattc tacgattttt catctcttcc atataagata gtggtatgtc gtgaaatgga
      721 aaaccttcat ctgatgctat gtatcccatt gcatgatgtg aaaaaattaa aatatctttt
      781 tcatctttat ctataacttg cttaattatt tctttatcag gaaaagtagt tgtgtatact
      841 ttattaatat tttgagcatt atctaatact aatcctatgt atttttttct aaattctgga
      901 gtaataaaat cattttcaaa attcataaaa ctccaatcat cattaatatt tttaatatta
      961 aattctaaat ctaatttttt ataaagttca attgccttaa taataaccac tcctttataa
     1021 taatattgaa ttactacata ttaatataga aatttccgat aataaacttt atacgtttaa
     1081 gt
//
LOCUS       DAYAPA010000269         1067 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.274, whole genome shotgun sequence.
ACCESSION   DAYAPA010000269 DAYAPA010000000
VERSION     DAYAPA010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1067
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.274"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            100..447
                     /locus_tag="ACGI59_003860"
     CDS             100..447
                     /locus_tag="ACGI59_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFG9741573.1"
                     /translation="MHENKKNLLDGSLAQYNDTAVPKIPVFAGNDITSEVYYFKPNQV
                     LNAHRHPNGEQIFVFLKGEGKMKLGEHECDVKNGDTVFVPTGEWHEITNGSNEEMVAV
                     QITKINAGAEYRG"
     gene            complement(597..>1067)
                     /locus_tag="ACGI59_003861"
     CDS             complement(597..>1067)
                     /locus_tag="ACGI59_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFG9741574.1"
                     /translation="AFAGGEGDFTTAFEPTATAMEKEGSGYIVTSIGKDSGEIPFTAY
                     STTRSYMKNNKDLIQRFTNAVYKGQLWIQQASSKEVAKAMQPFFEDISLEDLTTVVDR
                     YKSIDAWSQNPVLKEEGLKKLMTVMKEAKELDKEAPYKDIVNTEFANNAIKNIK"
BASE COUNT          371 a          154 c          154 g          388 t
ORIGIN      
        1 aataaaagaa ttttattcct attgtgtaaa gtgagaatac tattttctta tattggtata
       61 ataatagtaa atctaatata gaaaagaggt atttctagta tgcatgaaaa taaaaaaaat
      121 ctattagacg gaagcttagc tcaatataat gatacagcag ttccaaaaat accagtattt
      181 gctggtaacg atataacttc agaagtttat tattttaaac ctaatcaagt tcttaatgct
      241 catagacacc ctaatggtga acaaatattt gtgttcctaa aaggagaagg taaaatgaaa
      301 cttggtgagc atgaatgtga tgtaaaaaac ggcgatactg tatttgtacc aacaggagag
      361 tggcatgaaa ttactaatgg aagcaatgaa gaaatggtag ccgttcaaat tacaaaaatt
      421 aatgctggag cagaatacag aggatagata tataagtaca gttagtactt taatatctat
      481 tttattatat aaactttaat ctcaaatata aaaagagtct tttaaaaggc tctttttata
      541 tttttgttaa ttttcattat aaaaacaaga aaatatctag ctaaaactaa tatattttat
      601 ttaatatttt ttatagcatt gtttgcaaac tcagtattta cgatatcttt ataaggtgct
      661 tccttatcta gttctttagc ttctttcata acagtcatca atttcttcaa accttcctct
      721 tttaatacag gattttgaga ccatgcatct attgatttat atctatctac aactgttgtt
      781 aaatcttcta aagatatatc ttcaaagaaa ggttgcatag ccttagctac ttctttgcta
      841 gatgcttgtt gaatccataa ttgaccttta taaacagcat ttgtaaatct ttgaattaaa
      901 tctttattgt ttttcatgta tgatctcgta gtagagtatg ctgtaaaagg tatttctcca
      961 gaatcttttc ctatagatgt aactatatat ccactacctt ctttttccat tgctgttgct
     1021 gtaggttcaa atgctgttgt aaagtcaccc tctccacctg caaaagc
//
LOCUS       DAYAPA010000270         1021 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.275, whole genome shotgun sequence.
ACCESSION   DAYAPA010000270 DAYAPA010000000
VERSION     DAYAPA010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1021
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.275"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            <1..573
                     /locus_tag="ACGI59_003862"
     CDS             <1..573
                     /locus_tag="ACGI59_003862"
                     /inference="COORDINATES: protein motif:HMM:NF040238.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5906 domain-containing protein"
                     /protein_id="HFG9741575.1"
                     /translation="IFKKLVSGDRVNVERKGQNPFDFNNYSKFLFSANNIPRIKDKTG
                     AVLDRLIIIPFNASFSVNDKDFDPYIKYKLREQEAIEYLINLGLEGLKRVLKNRKFTV
                     PDKVKKEILEYEEVNNPILGFFKEVDKIENESTKEIYRKYQEYCILNGLQPISNIEFS
                     RQVVKKFGYEVKDKRIQGKKYKVFTRAVPG"
BASE COUNT          423 a          100 c          174 g          324 t
ORIGIN      
        1 atatttaaaa agctagtaag tggcgataga gtaaatgttg aaagaaaagg tcagaatcca
       61 tttgatttta acaactatag taagttttta ttttcagcta acaacatacc tagaataaaa
      121 gataagactg gagcagtact tgatagatta attattattc cttttaatgc tagtttttca
      181 gtaaatgata aggattttga cccttatata aagtacaaat taagagagca agaagctata
      241 gaatatttaa taaatctagg attagaaggc ttaaaaagag tattgaagaa tagaaaattt
      301 acagtaccag ataaggttaa aaaagaaata cttgaatatg aagaagtcaa taatcctatt
      361 ttaggatttt ttaaagaagt tgataagata gaaaatgaat caacaaaaga aatatatagg
      421 aaatatcaag agtattgtat attaaatgga ttacaaccta tatcaaacat tgagttttca
      481 agacaagtag tcaaaaagtt tggatatgaa gtaaaagata aaagaataca aggtaagaaa
      541 tataaagtat ttactagagc agtcccggga tagtcccgga tgtagtccct agatgtaata
      601 tagtaatttc aacgttgtcc cggatgtccc tagatgtttt tgacctttta tatatttaac
      661 taaaaaatca tcaatttttt gtgatgattt tgctcccttt atataaagaa tatatatata
      721 atagtatggg actgaacaaa tgttattgtc tggatatatt gaaatagcta gacttggagc
      781 agtcccggat gtattttaca tctgggacta tctgggactg ggtttaatta agaagttata
      841 caatataaaa atatttaaaa aataagctat atttcattaa taacttaaca aaataattaa
      901 aaagaaaatt ctgttaattt gcaaggatgt gaaaatgaag tgacagacta tgaaaaatta
      961 gaagaattgt taaactcata tagtaatttg gatattgaaa ttagagaaat agagttaaaa
     1021 g
//
LOCUS       DAYAPA010000271          994 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.276, whole genome shotgun sequence.
ACCESSION   DAYAPA010000271 DAYAPA010000000
VERSION     DAYAPA010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..994
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.276"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            610..915
                     /locus_tag="ACGI59_003863"
     CDS             610..915
                     /locus_tag="ACGI59_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9741576.1"
                     /translation="MPSMIMEVVGVLFIAIGLILTFIEIKGNFPSFYGFGQNSIGKNA
                     VGIIAAGIALIFFRNGIDSIDSIATYGVFLAIELVVIVAYFLIRKKFTKEDKHLRTN"
BASE COUNT          455 a           89 c          122 g          328 t
ORIGIN      
        1 cttataaata aaaaccaagc taatagatta gagaatgaat taattataga taaaaatcaa
       61 gctcatagat taaaaaataa atgatttata gatgaagaac taaacttata gattaaaaaa
      121 taaatgactt atttatagat gaaaaaccaa acttatagat taaaaaataa atgatttata
      181 gatgaagaac taaacttata gattaaaaaa tgacttattt atagatgaaa aaccaaactc
      241 atagattaaa aaataaatga cttatagatg aaaaatcaag ctcatagatt aaagaataaa
      301 taacttatta gatgaaaaac caagcttata gataagaagt ccatgcttat aagccagaaa
      361 atgaatactt taatagatta aaaaatacat atttgtgaat tatagaatga atgactccat
      421 aaactagaaa atttataata atatctatat aaaaattaga agtattttat cacaaaaaaa
      481 ttaagttaaa taaatatgaa attacttcca aatatacttc caaaaacatg taatcgcata
      541 tataatataa ttataaaaat aataaatatt tttatagtag tagttaatta ttaataagga
      601 ggggtcaaaa tgcctagcat gattatggaa gttgtaggag tactatttat agctattgga
      661 ttaatcttga cattcattga aataaaagga aatttccctt ctttttatgg ttttggtcaa
      721 aattctatag ggaaaaatgc tgtaggtatt atagcagcag gaattgcttt aattttcttt
      781 agaaatggca tagatagcat agattctata gctacttatg gtgtattttt agcaatagaa
      841 cttgttgtaa tagttgcata tttcttaata agaaaaaaat ttactaaaga agataaacat
      901 ttaagaacta attaactaaa ttatctacta taaaattgga aaattaaaaa caaataaaag
      961 tttgttttta attatttaaa atccaaattt gatt
//
LOCUS       DAYAPA010000272          983 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.277, whole genome shotgun sequence.
ACCESSION   DAYAPA010000272 DAYAPA010000000
VERSION     DAYAPA010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..983
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.277"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     regulatory      38..131
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            214..699
                     /locus_tag="ACGI59_003864"
     CDS             214..699
                     /locus_tag="ACGI59_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HFG9741577.1"
                     /translation="MISELMLKNFRKSSINFKTKIKLNLREKPDINSLKLKSIPEGKI
                     VKLKCVDGIWAEVESNYDKGWLLYKYLERLSNAKNNSNDTIKNRKKNYIGNIRTNGLS
                     LELRNDRTLESKVITTIPDGFKVEVCYSVGKWARVNINKNEKRYSGYVYNQYIEEHLS
                     L"
BASE COUNT          440 a           79 c          143 g          321 t
ORIGIN      
        1 gtaatattat tttttaattg ttcaaattat gttattattc agatataggc aatattagat
       61 aaatctaatg acgcaaaact atagggacta aaatctttat gtaataaagg tcatgtcagc
      121 cagttgccaa aaagataata attctttttg ttaaaagtat ttttagagtt atatatttta
      181 ataacttaat atttggcaag aggagggctt aagatgatat ctgaattaat gttaaaaaat
      241 tttcgcaaat ctagcatcaa ttttaagact aaaattaaat tgaatttaag agaaaaacca
      301 gacataaata gtttgaaatt aaaaagtata ccagaaggta aaattgttaa gttaaagtgt
      361 gtagatggaa tatgggcaga agttgagagt aattatgaca aaggttggtt actttataaa
      421 tatttagaaa gattaagtaa tgctaaaaac aatagtaatg atacaataaa aaatagaaaa
      481 aagaattaca taggaaatat aagaacaaat ggattaagtt tagaacttag aaatgataga
      541 acattagaat ctaaagttat aactacaatc cctgatggtt ttaaagttga agtatgttat
      601 agcgttggaa aatgggcaag agttaatatc aataaaaatg aaaaacgata ttctggatat
      661 gtatataatc aatacataga agagcattta agtttataat acgatactag caagctttaa
      721 atatattaat aaatcgcaaa aaagaatccc attaatggga ttcttttttg tagaaacatt
      781 ataacagaac taaagagaat aaattaacta atttattttt attataaagt aattataaat
      841 ggtaaattta attgaaagta gatttaactt atactttata caccaagtat cataaattaa
      901 tactactttg aaaaatctta aaaaaagtaa aagttatgat aataaaaata atattaaaga
      961 taataagata tataagaaac ttg
//
LOCUS       DAYAPA010000273          846 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.278, whole genome shotgun sequence.
ACCESSION   DAYAPA010000273 DAYAPA010000000
VERSION     DAYAPA010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..846
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.278"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
BASE COUNT          358 a           91 c           79 g          318 t
ORIGIN      
        1 taattagact tctctcttat tcaagatttt tctatataaa atctaagaat aaatagaaaa
       61 ctaacatcta accttttatt ttttattttc gatctattta cttgaatttt aacataaatc
      121 tatcttttag tatatattaa tcatgctgaa tttgtgatga catgtataca aaatatcgtt
      181 tagtattttc taataaaaaa tattactgaa attaaaaatc tacaatagca tttaacaagt
      241 aaaaacatgt ttacacaaat atataattta aggtctttaa aatagagtat gtattattct
      301 aaaaattagt tatagttatc acataaaaat agaaaagaat tttcaaaaga tttaaagaac
      361 atatatactt caattgcaga agatgtaatg ttaagtgaac tagcattctt cgaggaaaaa
      421 tagactcaaa aaattagaaa atagaatcag gttttagatg atttaagtat ttattttcaa
      481 aaaagaaatt tgattctaag ttaataaaga aatatttaaa aaaatcaaaa aattttctta
      541 aataagtaat aggttttgta aaaatggaaa aatatcaaaa taagactatg atttttaaaa
      601 ctatagcctt atcaataaat ttttatttaa agaacacaca taaatatttt taaacctaat
      661 ttatgtgaag ttttatgtat tttttatata agtagtattc taatcttact catctaatat
      721 accaaaaaga ctgtaagtaa gttcactttt atgcaaactt acccacaatc tcttttacac
      781 attaattcta tttattaaaa ttaaaaatat ttactgctat tctacccaat aaagatgata
      841 ctgttg
//
LOCUS       DAYAPA010000274          807 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.279, whole genome shotgun sequence.
ACCESSION   DAYAPA010000274 DAYAPA010000000
VERSION     DAYAPA010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..807
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.279"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(303..782)
                     /locus_tag="ACGI59_003865"
     CDS             complement(303..782)
                     /locus_tag="ACGI59_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HFG9741578.1"
                     /translation="MSNKLIKNLLSGILQILFFLLGLVIVAGGFKSFMYLCFSGEATL
                     QGTIYGILMFILGVSYFIIIKSLIEVLGSSEYSLFVKENVKRFRIIGYLLLLNSLIEF
                     ISTFGTTGKGMRFLDLGFGFYFTVPVFVYFITSLMSFVIADGFVKAIKIKEDNDLTI"
BASE COUNT          322 a          117 c           58 g          310 t
ORIGIN      
        1 aaacatctat tttatactga ttatttacaa atatatctaa attaattata aattttattg
       61 aataagactt ttataattat acctttcata aattataaaa aagtactttt ataaaaaaat
      121 gtgcttttaa tatctactaa taaagttctt tattttaatt aaaacataat tatattttag
      181 tattataatc ttttaatttt agcattatag tttatgtatt ataccctaat atattttatt
      241 ttaaattact agttagtcac tcttttcggg ttatttatct cctatatatt aaagtgtcac
      301 atctaaatag ttaaatcatt atcctctttt atctttattg cctttacaaa tccatctgca
      361 attacaaaac tcataagaga agttataaaa tatacaaata ctggaacagt aaaataaaat
      421 ccaaatccta aatctagaaa tctcattcct tttccagtag taccaaaagt tgaaataaac
      481 tcaattagag agtttaaaag caaaagatat ccaataatcc taaatctttt tacattctct
      541 tttacaaata ggctatattc agaactacct aatacttcta tcaatgattt tataatgata
      601 aaatatgata cacctaaaat aaacattaat attccataaa tagtaccttg tagagttgct
      661 tctccactaa aacagaggta cataaaactt ttaaatcctc ctgcaacaat tactaatcct
      721 aaaagaaaaa ataaaatttg taatatacca cttagtaaat tttttataag tttattagac
      781 atcatgccaa ctcctttaaa atatttt
//
LOCUS       DAYAPA010000275          790 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.280, whole genome shotgun sequence.
ACCESSION   DAYAPA010000275 DAYAPA010000000
VERSION     DAYAPA010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..790
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.280"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            <1..>790
                     /locus_tag="ACGI59_003866"
     CDS             <1..>790
                     /locus_tag="ACGI59_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFG9741579.1"
                     /translation="PDKFSLQNALKDLDNAYKKFFKEKAGFPKFKSKKINRFSYKTNF
                     TNGNIMYCGQYIKLPKLGMVKVRDKQVPQGRILNATISKEPSGRYYVSLCCTNVDIEV
                     FENTNNQIGLDLGIKEFCISSCGDFIENPKYLKKSLNKLAKLQRELSRKTIGSLNRNK
                     ARLKVARLQEHIANQRNDFLQKLSTKLIKENDIICIEDLQVKNMIRNRKLSRLISDVS
                     WSEFVRQLKYKANWHGRQIVKVGKFFASSQICNKCGYKNEEMKDL"
BASE COUNT          336 a           89 c          125 g          240 t
ORIGIN      
        1 cctgacaaat tttcactaca aaacgcctta aaagatttag ataatgcata taaaaaattc
       61 tttaaagaaa aagcaggatt tcctaaattt aaatcaaaga aaattaatag attttcatat
      121 aaaactaact ttacaaatgg aaacattatg tattgtggtc aatatataaa actacctaaa
      181 cttggtatgg ttaaggtaag ggataaacaa gtacctcaag gtagaatact taatgctact
      241 atatctaaag aaccaagtgg tagatattat gtatcattat gctgtactaa tgtagatatt
      301 gaagtatttg aaaatactaa taatcagata ggtttagatt taggaattaa agaattttgt
      361 atctcaagtt gtggagactt tatagaaaat cctaagtatc ttaagaagtc attaaacaaa
      421 cttgctaaat tacaaagaga attatcgaga aagacaatcg gtagtttaaa tagaaataaa
      481 gcaagattaa aagttgcaag acttcaagaa catatagcta atcaaagaaa tgattttcta
      541 caaaaactat ctactaaatt aataaaagag aatgatatta tttgcataga agatttacaa
      601 gtaaagaata tgattagaaa tcgtaaacta tctcgcttaa tttctgatgt gtcatggtca
      661 gaatttgttc gtcaattaaa atacaaggct aattggcatg gtagacaaat tgtaaaggta
      721 ggtaaattct ttgcaagttc acaaatatgc aataaatgtg ggtataaaaa cgaggaaatg
      781 aaggacttaa
//
LOCUS       DAYAPA010000276          787 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.281, whole genome shotgun sequence.
ACCESSION   DAYAPA010000276 DAYAPA010000000
VERSION     DAYAPA010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..787
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.281"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(<1..>787)
                     /locus_tag="ACGI59_003867"
     rRNA            complement(<1..>787)
                     /locus_tag="ACGI59_003867"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          172 a          238 c          182 g          195 t
ORIGIN      
        1 cgtccatccc ggtcctctcg tactaaggac aggtcttctc aaatttccta cgcctgcgac
       61 ggatagggac cgaactgtct cacgacgttc tgaacccagc tcgcgtacca ctttaatggg
      121 cgaacagccc aacccttggg acctactaca gccccaggat gtgatgagcc gacatcgagg
      181 tgccaaacct ccccgtcgat gtggactctt gggggagata agcctgttat ccccagggta
      241 gcttttatcc gttgagcgat ggcccttcca cgcagaacca ccggatcact aagtccgatt
      301 ttcatccttg ctcgacctgt atgtcttgca atcaagctct cttttgcctt tacactctac
      361 gtacgatttc cgaccgtact gagagaacct ttgagcgcct ccgttacctt ttgggaggcg
      421 accgccccag tcaaactgcc cacctgacag tgtcccaaga ccagattcat ggcctatggt
      481 tagagtccca gtactacaag ggtggtatcc caaggatggc tccgccaaaa ctggcgtcct
      541 ggtttcaaag cctcccacct atcctgtaca tgtagtacca agacccaatg tcaagctaca
      601 gtaaagctcc atggggtctt tccgtcctgt cgcaggtatc cggcatcttc accggaatta
      661 caatttcacc gagtctgttg ttgagacagt gcccaaatcg ttacgccttt cgtgcgggtc
      721 ggaacttacc cgacaaggaa tttcgctacc ttaggaccgt tatagttacg gccgccgttt
      781 actgggg
//
LOCUS       DAYAPA010000277          764 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.282, whole genome shotgun sequence.
ACCESSION   DAYAPA010000277 DAYAPA010000000
VERSION     DAYAPA010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..764
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.282"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(109..>764)
                     /locus_tag="ACGI59_003868"
     CDS             complement(109..>764)
                     /locus_tag="ACGI59_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="collagen-like protein"
                     /protein_id="HFG9741580.1"
                     /translation="VTGEIGPTGATGPTGVTGEIGPTGNTGATGSIGPTGVTGPTGAT
                     GSIGPTGATGATGVTGPTGPTGATGNSSQPVANFLVNAPSPQTLNNGDAITGWQTIIG
                     NSSSITVDANGTFTVQENGVYYISVSVALQPGSSSINQYSFAILFPILGGKDLAGLTT
                     EPGGGGVLSGYFAGFLFGGTTFTINNFSSTTVGIRNGQSAGTAATLTIFRIADTVMT"
BASE COUNT          192 a          169 c          129 g          274 t
ORIGIN      
        1 tattattgtt attttattca ttctaaaata ggtaaaactc aatatataaa aatacttttg
       61 tcatttaaat aaagcctaaa tagtttttaa gattatttta gacacgtttt aagtcataac
      121 agtatcagct attctaaata tcgtcaaagt agccgcagtt cctgctgatt gcccatttcg
      181 tatccctact gttgtagatg aaaaattatt tattgtaaaa gtagtcccac caaataaaaa
      241 accagcaaaa tatccagaaa gtactcctcc gcctcctggc tcagtagtaa gccctgccaa
      301 atcttttcct cctaaaattg ggaataggat agcgaaagaa tattgattta tacttgatga
      361 acctggttgt aatgctactg aaactgatat ataatacaca ccattttctt gtactgtaaa
      421 cgtaccattt gcatctactg ttatacttga actatttcct attattgttt gccaacctgt
      481 tatagcatct ccattattta gtgtttgtgg agatggtgca tttacgagga agttagcaac
      541 tggctgagag gaattgcctg ttgctcctgt tggacctgtt gggcctgtta ctcctgtcgc
      601 tcctgttgct cccgttggtc ctatacttcc tgtcgctcct gttggacctg ttactcccgt
      661 tggccctata cttcctgtcg ctcctgtatt tcctgttggc cctatttctc ctgtcactcc
      721 tgttgggcct gttgctcccg ttggtcctat ttctcctgtc actc
//
LOCUS       DAYAPA010000278          753 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.283, whole genome shotgun sequence.
ACCESSION   DAYAPA010000278 DAYAPA010000000
VERSION     DAYAPA010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..753
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.283"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            <1..>753
                     /locus_tag="ACGI59_003869"
     rRNA            <1..>753
                     /locus_tag="ACGI59_003869"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          207 a          156 c          227 g          163 t
ORIGIN      
        1 tttaactgag cgttcagtta cttgacgtag acccgaaacc gggcgaccta cccatgagca
       61 ggatgaagcg aaagtaaaat ttcgtggagg tccgaaccca cgagcgttga aaagctcggg
      121 gatgacttgt gggtagcggt gaaattccaa tcgagcccgg agatagctgg ttctccccga
      181 aatagcttta gggctagcct caaggtgaga gatacggagg tagagcactg aatgtcctag
      241 ggggtattgc acctaccgaa gactatcaaa ctccgaatgc cgtcatctta tacttgggag
      301 tcagactgtg ggtgataaga ttcatagtcg aaagggcaac agcccagatc gtcagctaag
      361 gtccctaaat gtaagttaag tggtaaagga tgtgggattg cacagacaac caggatgttg
      421 gcttagaagc agccactcat tcaaagagtg cgtaatagct cactggtcga gtgatcctgc
      481 gccgaagatt tccggggcta aaacttacta ccgaagctac ggcatcagta atgatgggta
      541 ggggagcttc ccatacgggt tgaagcatga ccgtaaggac atgtggacag tatgggagtg
      601 agaatgttgg catgagtagc gagatgtggg tgagaatccc acaggccgta aacccaaggt
      661 ttccagggga aggttcgtcc gccctgggtt agtcgggacc taagctgagg ccgaaaggcg
      721 taggtgatgg acaacaggtt gatattcctg tac
//
LOCUS       DAYAPA010000279          730 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.284, whole genome shotgun sequence.
ACCESSION   DAYAPA010000279 DAYAPA010000000
VERSION     DAYAPA010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..730
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.284"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(<1..254)
                     /locus_tag="ACGI59_003870"
     CDS             complement(<1..254)
                     /locus_tag="ACGI59_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9741581.1"
                     /translation="MSGIISVMTQSLILSIMALGVYITYKILDFPDMSADGSYTMGAS
                     IVAFSLTNGISPVVATLMAILCGCTAGLVTGILHIKFKIS"
BASE COUNT          299 a          109 c           86 g          236 t
ORIGIN      
        1 ttagatatct taaacttaat gtgaagaatc cctgtaacaa gaccagcagt acaaccacat
       61 aagatagcca ttaaagttgc tactacagga gaaataccat tagtaagaga aaatgctaca
      121 atagaagcac ccattgtata gctaccatca gcagacatat ctggaaaatc taagattttg
      181 taagttatat aaacccccag tgccataatc gataaaatta aactttgcgt cattacagat
      241 ataatacctg acataaataa aacacccctt tgaaaatatt ttaaatggtt tttaaaattt
      301 ggttttaaaa aatgattttg atataaaaaa accacacagt atatataact gtatggcaaa
      361 aattcgaata agccacacag tataatccat gtggctctat attatgatga taaaataaaa
      421 actttaacaa caattactat agccaacata gatacaatcc ttaaaaatca taatcctaag
      481 gggcttgtat ctatgttatg acaatttgtc ttaacttagg tacaaaccct aactaaattg
      541 gctactaaaa aatcgtactt gtttgtttaa taaagtcttc atatttacct ctccttttaa
      601 tttaataatt ttgaaaattt tgttttctgt tttgtatagt aattagttta atacagtaaa
      661 aaataaaagt caactataaa ttttgaaaaa ttatcaaatt atcagaaaat tgagtttaaa
      721 taaaaaaatt
//
LOCUS       DAYAPA010000280          669 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.285, whole genome shotgun sequence.
ACCESSION   DAYAPA010000280 DAYAPA010000000
VERSION     DAYAPA010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..669
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.285"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            69..>669
                     /locus_tag="ACGI59_003871"
     CDS             69..>669
                     /locus_tag="ACGI59_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFG9741582.1"
                     /translation="MLDAFITIVSSIIEIWSCKKVFDYTSQSKTDLKKLNMYFLFSVF
                     LIISSLYINIGANSRILICIFVMFVFYKSNYKVNIYKCLVISFFYWLILIGINGLSML
                     LVATVSNLDNMNVLLSGSLYRYEAIFLGKIMLGILLYVYKASFKNFKISKKEIYICIP
                     MIANLLSFFVLYDYIINLVKISPERRTAFLVIVLLLILSN"
BASE COUNT          236 a           55 c           87 g          291 t
ORIGIN      
        1 aattagtatg tattttttat gatatatttt ataaaaaata taatttaatc atttttgagg
       61 atattattat gttagatgca tttataacaa ttgtttcttc tattatagaa atatggtcat
      121 gtaaaaaagt ttttgattat actagtcagt caaagaccga cttaaaaaaa ctaaatatgt
      181 attttctctt ttctgttttt ctcattattt cttctttata tataaatata ggagctaata
      241 gtagaatctt gatatgtata tttgtaatgt tcgttttcta taagtctaac tataaggtta
      301 atatatataa atgtctggta atctcatttt tttattggtt aatattaata ggtataaatg
      361 gattaagtat gttattagta gcaacagtaa gcaatttgga taatatgaat gttttactta
      421 gtggcagttt atatagatat gaagcaatat tcttgggaaa gataatgtta ggtatattac
      481 tttatgttta taaggcttct tttaaaaatt ttaaaataag taaaaaagag atatacatat
      541 gtattcctat gatagcaaat ttattgtcat tttttgtact ttatgattat attattaatc
      601 ttgtaaaaat ctctcctgaa agaagaactg cttttttagt aatagtttta ctattaatat
      661 tatctaaca
//
LOCUS       DAYAPA010000281          664 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.286, whole genome shotgun sequence.
ACCESSION   DAYAPA010000281 DAYAPA010000000
VERSION     DAYAPA010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..664
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.286"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(<1..>664)
                     /locus_tag="ACGI59_003872"
     rRNA            complement(<1..>664)
                     /locus_tag="ACGI59_003872"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          152 a          191 c          151 g          170 t
ORIGIN      
        1 gatccagccg caccttccga tacggctacc ttgttacgac ttcaccccag ttattgattc
       61 caccttcgac gacttcttcc aaaaggttag ataatcggct tcgggcgtct ccaactcccg
      121 tggtgtgacg ggcggtgtgt acaagacccg ggaacgcatt caccgcagca ttctgatctg
      181 cgattactag taactccagc ttcatgtagg cgagtttcag cctacaatcc gaactgagag
      241 tagctttaag ggattagctc caccttacgg cttggcaacc ctctgtacta cccattgtag
      301 cacgtgtgta gccctaagca taaggggcat gatgatttga cgtcatcccc accttcctcc
      361 aggttatccc tggcagtctc tctagagtgc ccaacttaat gatggcaact aaagacaagg
      421 gttgcgctcg ttgcgggact taacccaaca tctcacgaca cgagctgacg acaaccatgc
      481 accacctgtc accaatgtcc ccgaagggaa ctctccgatt aaggagatgt cattgggatg
      541 tcaagcttag gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc cgctacttgt
      601 gcgggtcccc gtcaattcct ttgagtttca ctcttgcgag cgtacttccc aggcggagta
      661 ctta
//
LOCUS       DAYAPA010000282          607 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.287, whole genome shotgun sequence.
ACCESSION   DAYAPA010000282 DAYAPA010000000
VERSION     DAYAPA010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..607
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.287"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            101..391
                     /gene="cas2"
                     /locus_tag="ACGI59_003873"
     CDS             101..391
                     /gene="cas2"
                     /locus_tag="ACGI59_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HFG9741583.1"
                     /translation="MKKKNNLNYNYVFVVYDVGEKRVSKVFKVCKKYLSHFQKSVFRG
                     EITPSKIMSLKRDLENIIDKDNDFVCIIKLMNSNTFEEEILGNNSNCENMIL"
BASE COUNT          255 a           38 c           91 g          223 t
ORIGIN      
        1 agtattttaa gatgagaaag gagaaaatac tgaaaaataa ttattctatt ataatttgcc
       61 aaggttttgt atatttttca gtttaaatat tatattacat atgaaaaaga aaaacaattt
      121 aaattataat tatgtatttg tagtctatga tgtaggagaa aaaagagtaa gtaaagtttt
      181 taaagtgtgt aaaaaatatc tctcgcattt tcaaaaatca gtttttagag gtgaaataac
      241 tccatctaag attatgagct taaaacgtga tttagaaaat attatagata aagataatga
      301 ttttgtatgt ataataaagt tgatgaattc taatacattt gaagaagaaa tattagggaa
      361 taatagtaat tgtgaaaata tgatattata aatctaatta ctatatttgt aaaaaataat
      421 agcttattaa agagtagtaa aattgggata taatttatga tagagactgc aagtagattt
      481 ttgtgaaaag gatttacttg cagtattttt gttatgtata actgttaaca ttagtgtaca
      541 aaacattatc aatagcattt ttctataatg aagaaatact attataatgg gaaaatttta
      601 ttaatac
//
LOCUS       DAYAPA010000283          581 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.288, whole genome shotgun sequence.
ACCESSION   DAYAPA010000283 DAYAPA010000000
VERSION     DAYAPA010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..581
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.288"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            <1..429
                     /locus_tag="ACGI59_003874"
     CDS             <1..429
                     /locus_tag="ACGI59_003874"
                     /inference="COORDINATES: protein motif:HMM:NF024870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFG9741584.1"
                     /translation="KDITKKGGDKITAIKPNLQEKAANKIAGMVDIVARVVADGDVRT
                     LSFKTNEVIFGGGRLKVKSNEIPLDYNELMKVYEDISIEKNEIKKSSKPKKSKTNEEV
                     EMTPIVEETSEKTEKEQTEEEVIEQEEAIKPVKKTRKKRN"
BASE COUNT          284 a           43 c          115 g          139 t
ORIGIN      
        1 aaagatatta ccaaaaaagg tggtgacaag ataacagcaa tcaaacctaa tttacaagaa
       61 aaagcagcaa ataaaatagc aggtatggtt gatatagttg caagagtagt agctgatgga
      121 gacgttagaa cattatcatt taagactaat gaagtgatat ttggaggggg tagattaaaa
      181 gtaaaatcta atgaaattcc acttgattat aatgaattaa tgaaagtata tgaggatatt
      241 agtatagaga aaaatgaaat aaagaaatca agtaagccta aaaaatctaa aactaatgaa
      301 gaagtagaga tgactcctat agtagaagaa acaagtgaga aaactgaaaa agaacaaaca
      361 gaggaagaag taattgaaca agaagaagca attaaaccag tcaagaaaac tagaaaaaag
      421 agaaattagt ttgagtgatt atataagaaa gattattaat taaaaattta aaagaaaagg
      481 atggtatgtg atatggattt tagtaaattt gataaagcaa tagatgtaaa aggattaaaa
      541 gaagatataa aagaagcatc tgaaaatagt ggtaaattta a
//
LOCUS       DAYAPA010000284          581 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.289, whole genome shotgun sequence.
ACCESSION   DAYAPA010000284 DAYAPA010000000
VERSION     DAYAPA010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..581
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.289"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            <1..429
                     /locus_tag="ACGI59_003875"
     CDS             <1..429
                     /locus_tag="ACGI59_003875"
                     /inference="COORDINATES: protein motif:HMM:NF024870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFG9741585.1"
                     /translation="KDITKKGGDKITAIKPNLQEKAANKIAGMVDIVARVVADGDVRT
                     LSFKTNEVIFGGGRLKVKSNEIPLDYNELMKVYDDISIEKKDVKKSSKTKKSNAEEEV
                     EMTPTVEEISEKTEKEQTEEEVIEQEEAIKPVKKTRKKRS"
BASE COUNT          279 a           43 c          119 g          140 t
ORIGIN      
        1 aaagatatta ccaaaaaagg tggtgacaag ataacagcaa tcaaacctaa tttacaagaa
       61 aaagcagcaa ataaaatagc aggtatggtt gatatagttg caagagtagt agctgatgga
      121 gacgttagaa cattatcatt taagactaat gaagtgatat ttggaggggg tagattaaaa
      181 gtaaaatcta atgaaattcc acttgattat aatgaattaa tgaaagtata tgatgatatt
      241 agtatagaga aaaaagacgt taagaagtca agtaagacta aaaaatctaa tgctgaggaa
      301 gaagtagaaa tgactcctac agtagaagaa ataagtgaga aaactgaaaa agaacaaaca
      361 gaggaagaag taattgaaca agaagaagca attaaaccag tcaagaaaac tagaaaaaag
      421 agaagttagt ttaagtgatt atataagaaa gattattaat taaaaattta aaagaaaagg
      481 atggtatgtg atatggattt tagtaaattt gataaagcaa tagatgtaaa aggattgaaa
      541 gaagatataa aagaagcatc tgaaaatagt ggtaaattta a
//
LOCUS       DAYAPA010000285          577 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.290, whole genome shotgun sequence.
ACCESSION   DAYAPA010000285 DAYAPA010000000
VERSION     DAYAPA010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..577
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.290"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            <1..>577
                     /locus_tag="ACGI59_003876"
     rRNA            <1..>577
                     /locus_tag="ACGI59_003876"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          154 a          121 c          190 g          112 t
ORIGIN      
        1 caggatggac ccgcgtctga ttagctagtt ggtaaggtaa cggcttacca aggcgacgat
       61 cagtagccga cctgagaggg tgatcggcca cattggaact gagacacggt ccaaactcct
      121 acgggaggca gcagtgggga atattgcaca atgggcgaaa gcctgatgca gcaacgccgc
      181 gtgagtgatg aaggccttcg ggtcgtaaaa ctctgtcctc aaggaagata atgacggtac
      241 ttgaggagga agccccggct aactacgtgc cagcagccgc ggtaatacgt agggggctag
      301 cgttatccgg atttactggg cgtaaagggt gcgtaggcgg tctttcaagt caggagtgaa
      361 aggctacggc tcaaccgtag taagctcttg aaactgggag acttgagtgc aggagaggag
      421 agtggaattc ctagtgtagc ggtgaaatgc gtagatatta ggaggaacac cagttgcgaa
      481 ggcggctctc tggactgtaa ctgacgctga ggcacgaaag cgtggggagc aaacaggatt
      541 agataccctg gtagtccacg ctgtaaacga tgagtac
//
LOCUS       DAYAPA010000286          574 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.291, whole genome shotgun sequence.
ACCESSION   DAYAPA010000286 DAYAPA010000000
VERSION     DAYAPA010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..574
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.291"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            192..290
                     /locus_tag="ACGI59_003877"
     CDS             192..290
                     /locus_tag="ACGI59_003877"
                     /inference="COORDINATES: protein motif:HMM:NF022636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9741586.1"
                     /translation="MFILKRLCKLVSINKSFSEEIPKNLKNTYMCI"
BASE COUNT          221 a           44 c           67 g          242 t
ORIGIN      
        1 tatgagttgt aaaatattaa ataatattgt actaattgga ataattttaa ctttattata
       61 gattatttta ataccattag tactgatggt gtattttaaa attggtataa gcattacaaa
      121 tacgaatatg ccaactgtaa tatgtaatat aagtagctat atacatatgt gctgtcccat
      181 atattgtatc attgtttata ttaaaaagat tatgtaaatt ggtatcaatc aataaatcat
      241 tttcagaaga aatacctaaa aacttaaaaa atacctatat gtgtatttag ttagatttga
      301 gatttttaca tttaatttta tagtgacaat attatattat ttttatgatg tctatttata
      361 tgcaattact ataatatcag aacttatagt atcatttgtt tcattatcaa taaggttttt
      421 tactttagtt ttgtcaattt tattaaaaaa agttattgat ataaaagata agaaggataa
      481 aactatatga cggatttcaa acataagagt ggatatttat ataatagata ttttatagtt
      541 aattttatta gattttattt aatactgtaa tatt
//
LOCUS       DAYAPA010000287          530 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.292, whole genome shotgun sequence.
ACCESSION   DAYAPA010000287 DAYAPA010000000
VERSION     DAYAPA010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..530
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.292"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            <1..>530
                     /locus_tag="ACGI59_003878"
     CDS             <1..>530
                     /locus_tag="ACGI59_003878"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9741587.1"
                     /translation="DSKEIQEIPTYLTPIKNGLDFLSLGEGDGRNQALYNYILTLQSN
                     DFTIEEIRETIRVINKYVLKTPLQDNELEVILRDESFQKKIFFNSKGSFLFDEFAKYI
                     KNNNHVIKINEQLHLYKDGIYVDGQARIEAEMINNISNLNKAKRSEVLSYLNLLISEN
                     TSMSEANLIAFKNGIY"
BASE COUNT          227 a           44 c           87 g          172 t
ORIGIN      
        1 gatagtaaag aaatacaaga gataccaacg tatttaactc ctataaaaaa tggacttgat
       61 tttttaagtc ttggagaagg tgatggaaga aatcaagcat tatataatta tatattaact
      121 ctacagagta atgattttac tattgaggaa ataagagaaa caataagagt tattaataaa
      181 tatgttttaa aaactccatt acaagataat gaacttgaag taattttgag agacgaatcc
      241 tttcaaaaga agatattttt taattcaaaa ggttcatttt tatttgatga gtttgctaaa
      301 tacataaaaa ataataatca tgtaatcaag ataaatgagc agttacattt atataaggat
      361 ggtatttatg tagatggtca agcaagaata gaagcagaaa tgattaataa tataagcaat
      421 ttaaataagg ctaagaggag tgaagtactt agttatttaa atttattaat aagtgagaat
      481 acaagtatgt cagaagctaa tttaatcgct tttaagaatg gtatatataa
//
LOCUS       DAYAPA010000288          496 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.293, whole genome shotgun sequence.
ACCESSION   DAYAPA010000288 DAYAPA010000000
VERSION     DAYAPA010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..496
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.293"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            <1..>496
                     /locus_tag="ACGI59_003879"
     CDS             <1..>496
                     /locus_tag="ACGI59_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HFG9741588.1"
                     /translation="GTVATGRVERGVLKVQDEVELVGLTEAPRKVVVTGVEMFRKLLD
                     QAQAGDNIGALLRGVQRNEIERGQVLAKTGSVKAHTKFTAEVYVLKKEEGGRHTPFFD
                     GYRPQFYFRTTDVTGACKLPEGIEMVMPGDNVTMEVDLINSIVVEEGLRFSIREGGRT
                     VASGV"
BASE COUNT          213 a           61 c          125 g           97 t
ORIGIN      
        1 ggaacagttg caacaggaag agtggaaaga ggagtactaa aagtacaaga cgaagtagaa
       61 ttagtaggat taacagaagc accaagaaaa gtagtagtaa caggagtaga gatgttcaga
      121 aaattattag accaagcaca agcaggggat aatataggag cattattaag aggagtacaa
      181 agaaacgaga tagaaagagg acaagtacta gcaaagactg gatcagtaaa ggcacacaca
      241 aagtttacag cagaagtata tgtacttaaa aaagaagaag gtggaagaca tacaccattc
      301 tttgatggat atagaccaca attctatttc agaacaacag acgtaacagg agcttgtaag
      361 ttaccagaag gaatagagat ggtaatgcct ggagataacg taacaatgga agtagactta
      421 ataaactcaa tagttgtaga agagggatta agattctcaa taagagaagg tggaagaaca
      481 gtagcttcag gagttg
//
LOCUS       DAYAPA010000289          493 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.294, whole genome shotgun sequence.
ACCESSION   DAYAPA010000289 DAYAPA010000000
VERSION     DAYAPA010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.294"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            188..304
                     /gene="rrf"
                     /locus_tag="ACGI59_003880"
     rRNA            188..304
                     /gene="rrf"
                     /locus_tag="ACGI59_003880"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            310..398
                     /locus_tag="ACGI59_003881"
     tRNA            310..398
                     /locus_tag="ACGI59_003881"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:344..346,aa:Leu,seq:taa)
     gene            410..486
                     /locus_tag="ACGI59_003882"
     tRNA            410..486
                     /locus_tag="ACGI59_003882"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:444..446,aa:Ile,seq:cat)
BASE COUNT          156 a           91 c          109 g          137 t
ORIGIN      
        1 attcattaag atatatgtgc agtttttaga gtgctaactc tatattttta aacttgtcaa
       61 aacttatgaa tttttaatag ttttataagg caaaaaatcg aataaaaaaa gtattgacac
      121 aataagacaa agatgttatt ataatacttg tcttagaaaa aaagttaaat gaaaatttag
      181 acaataatct ggttataata gcagagagga tacacctgtt cccattccga acacagaagt
      241 taagctctct agcgctgatg gtacttggtg ggaaactgcc tgggagagta ggacatagcc
      301 aggtgatgtg ccgaagtggc ggaactggca gacgcacagg acttaaaatc ctgcgggact
      361 tacctctcgt accggttcga ttccggtctt cggcaccaca aattattgtg ggccattagc
      421 tcagttggtt agagcgcccg gctcataacc ggtaggtctg gggttcgagt ccctgatggc
      481 ccaccacaat aat
//
LOCUS       DAYAPA010000290          474 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.295, whole genome shotgun sequence.
ACCESSION   DAYAPA010000290 DAYAPA010000000
VERSION     DAYAPA010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..474
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.295"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(<1..>474)
                     /locus_tag="ACGI59_003883"
     CDS             complement(<1..>474)
                     /locus_tag="ACGI59_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9741589.1"
                     /translation="LLLNLSMDNSQNNQEAFSILFEINTLYEEYIGILIKSIWDNSFR
                     ETYIQDKSKFLLKNEQTGKKNFNLRPDIVLYDLKNEYEIIIDTKWKAIEVDSNVFYRS
                     SDIYQMYAYITAYENAKRCILLYPCIQKDKNYSSWKLSESFKGKFIEAKTVRLDD"
BASE COUNT          160 a           73 c           40 g          201 t
ORIGIN      
        1 tatcatctaa cctcacagtt tttgcttcta taaacttacc cttaaatgat tctgataatt
       61 tccaggaact ataattctta tctttttgaa tacaaggata taataatata catctttttg
      121 cattttcata tgctgttata tatgcataca tctggtatat atcacttgac ctataaaaaa
      181 catttgaatc tacttcaata gctttccatt tagtgtcaat tataatttca tattcatttt
      241 ttaagtcata taaaactata tccggtctta aattaaagtt tttcttacca gtttgttcat
      301 ttttcaaaag aaatttactc ttatcttgta tatatgtttc tctaaatgaa ttgtcccata
      361 tacttttgat taaaattcct atatattctt catacaaagt atttatttca aataatatag
      421 aaaatgcctc ttgattattt tgactattat ccatagacaa atttaaaagt agaa
//
LOCUS       DAYAPA010000291          458 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.296, whole genome shotgun sequence.
ACCESSION   DAYAPA010000291 DAYAPA010000000
VERSION     DAYAPA010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..458
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.296"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
BASE COUNT          194 a           48 c           35 g          181 t
ORIGIN      
        1 aatttttaga tttatatcac aatttagaat tatttgtaaa aaaggagatt atctttttat
       61 tagacaatcc ccttgtatat tttattaata tttatctatg attaagcatc aattttattt
      121 tatacttttt gattttaata ctatacatct aaatcttatt tagggggaac aattatttat
      181 atgatataaa aatgctaatt tgaatgaact tgtcttataa tcagttttag aaaatacacc
      241 attctacaaa gtatgaatca aaaggtgaaa aaatactaaa aataataaat caacatcaat
      301 tttaatcaat atatgaaaaa cttataccaa gtatgtataa aaactaatat aaatattatt
      361 ttaacaacta atacttacac ctttatatat tatatcatat ttcaataaat aaattgcata
      421 aaaggaaatc ttttcataga attaaactat ttattaat
//
LOCUS       DAYAPA010000292          454 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.297, whole genome shotgun sequence.
ACCESSION   DAYAPA010000292 DAYAPA010000000
VERSION     DAYAPA010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..454
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.297"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(<1..428)
                     /locus_tag="ACGI59_003884"
     CDS             complement(<1..428)
                     /locus_tag="ACGI59_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufBD protein"
                     /protein_id="HFG9741590.1"
                     /translation="MKNLNLLINKLYSKNHNEAYKTFLFLENESLKSNITYCFFDSFL
                     EMIDNENSYIRTRGLLLISANAQWDIDNKIEINIDSILSHIVDKKPSVSRMFIKSIPN
                     ITKYKENLINRIKMELSNADISIYNNNMKPLVEKDINDTLS"
BASE COUNT          152 a           59 c           45 g          198 t
ORIGIN      
        1 gataatgtat cgtttatatc cttttctact aatggtttca tattattatt ataaatactt
       61 atatctgcat tacttaattc catttttatg cgattaataa gattttcttt atactttgta
      121 atattaggta tggatttaat aaacattctt gatacagaag gttttttatc tacgatatga
      181 cttagtatac tatctatatt tatttcaatt ttattatcaa tatcccactg agcatttgca
      241 gagattaata acaatcccct tgttcgaata tatgagtttt cgttatcaat catttctagg
      301 aatgagtcaa aaaaacaata tgtaatattt gattttaaac tttcattttc taagaataaa
      361 aaagttttat atgcttcatt atgatttttt gaatataatt tattaataag taaattgaga
      421 ttcttcaatc ttctcatctc cctcattaca aata
//
LOCUS       DAYAPA010000293          412 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.298, whole genome shotgun sequence.
ACCESSION   DAYAPA010000293 DAYAPA010000000
VERSION     DAYAPA010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..412
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.298"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            32..>412
                     /gene="tcdR"
                     /locus_tag="ACGI59_003885"
     CDS             32..>412
                     /gene="tcdR"
                     /locus_tag="ACGI59_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin sigma factor TcdR"
                     /protein_id="HFG9741591.1"
                     /translation="MQKSFYELIVLARNNSVDDLQEILFMFKPLVKKLSRVLHYEEGE
                     TDLIIFFIELIKNIKLSSFSEKSDAIIVKYIHKSLLNKTFELSRRYSKMKFNFVEFDE
                     NILNMKNNYQSKSIFEEDICFFGYI"
BASE COUNT          167 a           28 c           60 g          157 t
ORIGIN      
        1 catcattata aatataagag aggatgattt tatgcaaaag tctttttatg aattaattgt
       61 tttagcaaga aataactcag tagatgattt gcaagaaatt ttatttatgt ttaagccatt
      121 agtaaaaaaa cttagtagag ttttacatta tgaagaggga gaaacagatt taataatatt
      181 ttttattgaa ttaataaaaa atattaaatt aagtagcttt tcagaaaaaa gcgatgctat
      241 tatagtcaaa tatattcata aatcattact gaataagact tttgagttgt ctagaagata
      301 ttctaaaatg aagtttaatt ttgtagaatt tgatgaaaat atcttaaata tgaaaaataa
      361 ttatcaaagt aagtctattt ttgaggaaga tatttgtttt ttcggatata tt
//
LOCUS       DAYAPA010000294          364 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.299, whole genome shotgun sequence.
ACCESSION   DAYAPA010000294 DAYAPA010000000
VERSION     DAYAPA010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..364
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.299"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            168..284
                     /gene="rrf"
                     /locus_tag="ACGI59_003886"
     rRNA            168..284
                     /gene="rrf"
                     /locus_tag="ACGI59_003886"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            291..362
                     /locus_tag="ACGI59_003887"
     tRNA            291..362
                     /locus_tag="ACGI59_003887"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:323..325,aa:Asn,seq:gtt)
BASE COUNT          117 a           61 c           78 g          108 t
ORIGIN      
        1 attcattaag atatatgtgc agtttttaga gtgttaactc tatgtaaagt actaacttca
       61 attgatgaag tatatatcgt aaaaacaatt actatttagt acttcaaaac tattgacatt
      121 taaaaataaa gatgttaata taatacttgt cttataaata ggcaatatgt ggttataata
      181 gcaaagagga tacacctgtt cccattccga acacagaagt taagctcttt agcgctgatg
      241 gtacttggtg ggaaactgcc tgggagagta ggacgtagcc acgtgaatat tccaaggtag
      301 ctcaatggtg gagcaaccgg ctgttaaccg gtaggctgtg ggttcgagcc ccacccttgg
      361 agcc
//
LOCUS       DAYAPA010000295          354 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.300, whole genome shotgun sequence.
ACCESSION   DAYAPA010000295 DAYAPA010000000
VERSION     DAYAPA010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..354
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.300"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     regulatory      complement(286..>354)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
BASE COUNT          120 a           42 c           48 g          144 t
ORIGIN      
        1 tattttcatg ggacttcatt aaaaaaatat tagttttctt actaaattga tacattattg
       61 tattaatgta atggaaatgc gattaattta aataaataaa gaagtcattt tttgtaaaat
      121 agtaactttt tgagataatt tttctttatt ttatgttata attggtaaaa tatgttatta
      181 tgtgtatttt ctactgaaat aaagtgattt ttccataaca aaatgactgc caaaaaggca
      241 gtcattatag acttttctta ggtatacaat acacctagga ctctttttgg caactggctg
      301 acatagttta tgtatataaa ccttagtccc tatagctttg cgtcactaga tttc
//
LOCUS       DAYAPA010000296          331 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.301, whole genome shotgun sequence.
ACCESSION   DAYAPA010000296 DAYAPA010000000
VERSION     DAYAPA010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..331
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.301"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(<1..>331)
                     /locus_tag="ACGI59_003888"
     rRNA            complement(<1..>331)
                     /locus_tag="ACGI59_003888"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           76 a           87 c           55 g          113 t
ORIGIN      
        1 aggactatta ccttctgtgg tgggtctttc caaacctctt caattacgat acctcttcgt
       61 tatgatatgt ccgcaacccc aataaagaaa actttattgg tttgggctaa tccgcgttcg
      121 ctcgccgcta cttacggaat cgaatttctt tctcttcctt caggtactta gatgtttcag
      181 ttccctgagt tcccctcatt aagctatgta ttcacttaat gatacttaga cattactcta
      241 agtgagtttc ctcattcgga aatcttcgga tcaaagttta cgtgcaactc cccgaagctt
      301 atcgcagctt atcacgtcct tcatcggctc c
//
LOCUS       DAYAPA010000297          328 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.302, whole genome shotgun sequence.
ACCESSION   DAYAPA010000297 DAYAPA010000000
VERSION     DAYAPA010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..328
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.302"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
BASE COUNT          133 a           31 c           68 g           96 t
ORIGIN      
        1 gtatattcat tgacaaatat aagttttaag tatatattaa aaattattag agtgtgcatt
       61 agaatgtata cagcgtatat taagaattat tagagtgtat ataaggatgt atacagtgta
      121 tattaaaaat tattaagggg agggggagtt tatgcaacaa aaatgtaaaa agaaagcact
      181 atcaatgatt ttaagtatct caatgggact tggaccagta gcatcattta atgcgtcaaa
      241 tactttcgca gatactatag aagaaagtac taatgcagga aatgcagaag gaggtactaa
      301 tgcaggaaat gcagaaggag atactaat
//
LOCUS       DAYAPA010000298          293 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.305, whole genome shotgun sequence.
ACCESSION   DAYAPA010000298 DAYAPA010000000
VERSION     DAYAPA010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..293
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.305"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            <1..>293
                     /locus_tag="ACGI59_003889"
     CDS             <1..>293
                     /locus_tag="ACGI59_003889"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9741592.1"
                     /translation="EVEINKMELSESKKGDPMFVCWFKILEGEYKDSLIFMNQVVKQG
                     FQIHIVNEFLRSLETDIEVEFESYSQYAQLIMDIAEEIDGELEFAIEYGEKKG"
BASE COUNT          119 a           24 c           61 g           89 t
ORIGIN      
        1 gaagtagaaa taaataaaat ggagttaagc gagtctaaaa aaggtgaccc aatgtttgta
       61 tgttggttta aaatactaga aggagagtat aaagattcat taatattcat gaatcaagta
      121 gttaagcaag gttttcaaat tcatatagta aatgaatttt taagaagttt agaaactgat
      181 attgaagttg aatttgaatc atacagccaa tatgcacaac tcataatgga tatagctgag
      241 gaaatagatg gagaacttga atttgctatt gaatatggtg agaagaaagg ttt
//
LOCUS       DAYAPA010000299          276 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.306, whole genome shotgun sequence.
ACCESSION   DAYAPA010000299 DAYAPA010000000
VERSION     DAYAPA010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..276
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.306"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(<1..>276)
                     /locus_tag="ACGI59_003890"
     CDS             complement(<1..>276)
                     /locus_tag="ACGI59_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycine-rich protein"
                     /protein_id="HFG9741593.1"
                     /translation="NDRHRPGGTQYQGGIGRVSTENGSFGKGCSAKDSTGEGGGGGWF
                     GGAGMNGAGAGGGGSGYVLTKDSYKPTGYTPTSEYYFDNVVMESGGNT"
BASE COUNT           72 a           85 c           40 g           79 t
ORIGIN      
        1 gtatttccac cagattccat aacaacatta tcaaaataat attcagatgt tggtgtgtag
       61 ccagtaggct tataactatc tttagttaat acgtagccac ttccacctcc acctgctccc
      121 gcaccattca ttcctgcacc accaaaccag ccacctccac cgccttcgcc agttgaatct
      181 ttagcagaac acccttttcc aaaacttccg ttttctgtgc ttacacgacc aataccacct
      241 tgatattgag taccgccggg acgatgtctg tcgtta
//
LOCUS       DAYAPA010000300          269 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.denovo.307, whole genome shotgun sequence.
ACCESSION   DAYAPA010000300 DAYAPA010000000
VERSION     DAYAPA010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..269
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.denovo.307"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            16..>269
                     /locus_tag="ACGI59_003891"
     CDS             16..>269
                     /locus_tag="ACGI59_003891"
                     /inference="COORDINATES: protein motif:HMM:NF024870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFG9741594.1"
                     /translation="MTKRQLAWDVFKEAIRELEKKENDFETIIVDLLEDTYEACRLYM
                     YDKMGITHESDDSFRAWDKVRTEFLSTIKRLMNLDYKNII"
BASE COUNT          109 a           29 c           58 g           73 t
ORIGIN      
        1 acagttgaag ggagaatgac aaagagacaa cttgcatggg atgtatttaa agaagctata
       61 agagaattag agaaaaaaga aaatgatttt gaaacaataa ttgttgactt gttagaagat
      121 acatacgaag cttgtcgcct ttacatgtat gacaagatgg gaataactca tgagtcagat
      181 gatagtttta gagcatggga taaggttaga acggagtttt taagcactat aaaacgactt
      241 atgaaccttg actataaaaa cataatatt
//
LOCUS       DAYAPA010000301        14964 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.guided.107, whole genome shotgun sequence.
ACCESSION   DAYAPA010000301 DAYAPA010000000
VERSION     DAYAPA010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14964
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.guided.107"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            complement(153..677)
                     /locus_tag="ACGI59_003892"
     CDS             complement(153..677)
                     /locus_tag="ACGI59_003892"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFG9741595.1"
                     /translation="MENKNPIVTIEMENGKEIKIELYPNIAPNTVKNFVSLVNEGYYN
                     GIIFHRVIPGFMIQGGCPNGTGMGGPGHSIKGEFSGNGFTNNLKHERGVISMARTMAP
                     NSAGSQFFIMHKNSPHLDGQYAGFGRVIEGMDTVDEIASVRTDSADKPQTPQVMKSVT
                     VETFGIDYSDVEKN"
     gene            complement(742..1494)
                     /locus_tag="ACGI59_003893"
     CDS             complement(742..1494)
                     /locus_tag="ACGI59_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFG9741596.1"
                     /translation="MKLGLCLEGGGAKGAYQAGVVKALYDGGINKFYSISGTSIGAIN
                     GYYLYTGNVDNLEKMWTNIKDIQNGNVKIVNNTVDNSPAIDNLRELDDSNIEEMNFYV
                     NYVEVDNKVVSEKIVDVSKMPRNEAIISISYSGLLPSNPNATLGFKEQFVKDVQEGIY
                     DGFKLDGGLIRSALIEPLIGDNVDKIILISTKYNYELPEDIKKVYDEDKIIVVRPNTQ
                     FAPKDTLNFDDEFCKTIYQEGYEIGKNILDRL"
     gene            complement(1525..2823)
                     /locus_tag="ACGI59_003894"
     CDS             complement(1525..2823)
                     /locus_tag="ACGI59_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HFG9741597.1"
                     /translation="MDSINFAKNLIDFIYDSPSSFHAVESTKEILDKNGFEELVLNQR
                     WNLRVGGKYYVTKNLSAIVAFVVNSEGIERDGFRIIGSHSDSPTFSIKPNAEIEAEKS
                     YLKLNTECYGGAILSTWLDRPLGIAGRVVLKGDSILKPNERLIDFKKPICIIPNLAIH
                     LNRSINDGHSYNKQKDMLPLVGMLNDTLEKDNFLIKQIASNLDVNMDDIIDFDLFLYE
                     FEKGSLIGANDEFISIGRQDNLAMVHASLNALVNTKGQHGVNVMAVFDNEEVGSSTKQ
                     GADSNMLLNILERICISMGKDREGFFESIYSSFMISSDLAHAIHPNIPEKHDPTNKPI
                     MGKGPVIKINASQAYTSDGYSIAIYKNICREANVEYQEFVNRSDERGGSTIGPISSTH
                     IDIPSVDVGSPILAMHSIRELGNVEDHYSIYKTFSKFFEI"
     gene            complement(2840..5104)
                     /locus_tag="ACGI59_003895"
     CDS             complement(2840..5104)
                     /locus_tag="ACGI59_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3553 domain-containing protein"
                     /protein_id="HFG9741598.1"
                     /translation="MNLDTLNPAQREAVEKTEGPVLILAGAGSGKTKVLTTRIAYLIE
                     DKGVQAPNILAITFTNKAANEMRERVEQNIGPETKDMWISTFHSCCVRILRKDINKIG
                     YNRSFVIYDSADQVTLVKDCLKELNLSDKVFEPKAVISAISGAKDKLYTPKQFKDINM
                     ADNRMVKIADIYALYQDRLKRNSALDFDDLILKTVELFKANDEVLAYYRSRFRYIMVD
                     EYQDTSKAQYELIKLLAREHQNICVVGDDDQSIYGWRGADIRNILEFEKDYDNVHVVK
                     LEQNYRSTQVILDAANKVISNNIERKRKKLWSEKKEGELIKIQLTGSEIEEADFIADS
                     IAQIARKENRPYKDFAVLYRANAQARPVEDALNRSQIPYNIYGGTKFYERKEIKDLLA
                     YLRVIQNPQDDISIKRIINVPRRGIGLRTIEKIEDRANLKQESIYSVLIDIETNSDIS
                     TKARASISGFVDIIGTLRTIKEVYPVSKLIEKVLDTTGYMDELVEIRNKNEKDLTGKG
                     EEAQDRIDNLREFISIALEFESSNDDTYENKDLETFLTSIALTSESNDEEDNDRVSLM
                     TIHTSKGLEFPVVFLTGMEEGLFPISRAIKSMSDSQIEEERRLCYVGITRAKEELYMS
                     LTEKRTLYGKTNVAIASRFMEELPEECIERLYKVKKELSYSKASYNMLDKYTKKYMST
                     ISKSKVADKVNATIKDSNKETNPDDIKLGSKVHHPKFGVGTVVSIIGTDVTIAFDQQG
                     IKKINKEYTTLNIL"
     gene            complement(5359..6177)
                     /locus_tag="ACGI59_003896"
     CDS             complement(5359..6177)
                     /locus_tag="ACGI59_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFG9741599.1"
                     /translation="MFKINNLTYQYEKNTNALLNINMDFSKGNVIGIIGSNGSGKSTL
                     FMNLMGILKPTSGEILFKEEKLKYDKRSLYNLRKNVGIVFQDPEKQIFYSKVYDDIAF
                     AMRNIGMDEKTIKERINKALVAVNGIDFIDRPVHFLSYGQKKRVAIASVIAMENEIVL
                     LDEPTAGLDPVSTRSIVDIIKGLNKNNIKIVISSHDMNLMYEICDYIYVLDKGILIDE
                     GKAENVFINENNIIQAGLESPWLVKVHRNMNLPLFKKEEDLYKYWKERELNTNK"
     gene            complement(6170..6868)
                     /gene="cbiQ"
                     /locus_tag="ACGI59_003897"
     CDS             complement(6170..6868)
                     /gene="cbiQ"
                     /locus_tag="ACGI59_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0032778 - P-type cobalt transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HFG9741600.1"
                     /translation="MLIIDKYAYTNRLSKVNPNKKVAIGVIFLIASMVIQNIFILSGI
                     MILMSILVVCVAGIDLKNYLKLLRIPMYFLFLSIGITLVNISFNKADLLYSFEFFSFN
                     VGISKASINMSIHVLFRAMSCLTCVYFCILTTPFNQLIFFFKKLHLPDTFVELSMLIY
                     RFIFIFLEEFSEIYKSQELRFGYINLKTSYKSLGILGSMLYKRLMTRYDDMCISLDIK
                     LYDGKFHIVGDNDV"
     gene            complement(6871..7155)
                     /locus_tag="ACGI59_003898"
     CDS             complement(6871..7155)
                     /locus_tag="ACGI59_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     substrate-binding protein"
                     /protein_id="HFG9741601.1"
                     /translation="MSAKTKTKTNILLLLLVVALIIFPLLVNSGAEYGGADGQAESEI
                     TKINPNYKPWFSSPYEPPSGEIESLLFSAQAALGAGVIGYILGLQKGKRS"
     gene            complement(7145..7897)
                     /locus_tag="ACGI59_003899"
     CDS             complement(7145..7897)
                     /locus_tag="ACGI59_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0000041 - transition metal ion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HFG9741602.1"
                     /translation="MKQNIKLGVIAALMLIILTPVTSNAMHIMEGYLPVKWSIAWGVI
                     FIPFFLFGLKSIGKIVKQDPKKKVLLALCGAFVFVLSALKIPSVTGSCSHPTGVGLGA
                     IMFGPSVMFVLGTIVLIFQALLLAHGGITTLGANAFSMAIIGPIVSFLIFKALKKKDG
                     NNAIPVFLAAAIGDLATYTITSIQLALAFPDPSGGVMASAIKFLGIFFMTQIPIAIAE
                     GILTVIVYNLITENGEKSILENNDKGVKANEC"
     regulatory      complement(8079..8271)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(8403..10175)
                     /locus_tag="ACGI59_003900"
     CDS             complement(8403..10175)
                     /locus_tag="ACGI59_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase activation-like protein"
                     /protein_id="HFG9741603.1"
                     /translation="MKWIYDDYEFENRANNLSWTIAGDYNESIDISEKDYSSKEIGLY
                     FAIIAGARRKYVDWDIVKKYLTNRIRKGYNKDVILGLIQVILNTIVEEKVIKDRPGIE
                     DIRRNAYKDIVSRFTKIHNDNIMDKIKYTFVLYSMDKHPALDSMTRRIVNDIRNIDTN
                     QDIMEILKALDVIYLTYFEYILNGEENSLIDENNVESVEVNFDTFADFMYEELYSDEE
                     ENIIESDIDSISSSMLVEGVGDFDDSKCSNSADRVIYVDEDTANKIYSKIEHYYGKTY
                     LSKGEIKKIETQNCRNVHEGCRIHFTDGVLRSECNNVFQLKYVTRQKENNLGKFREHA
                     RMYRQQIKRLKESLSRILIEENAKSRVYSDCGTILANRAWRVGRSNNNKIFYKDIENE
                     KGKYVIDILLDASGSQSRNQGNVAIQAYIIANALTEVGIPNRVMGFSSFLDYTILKRF
                     KDYDDSIKNSENIFEYFCAGNNRDGLAIKSICSGLINRDEENKILIVLSDGRPNDVKI
                     GKSSERTLRGEKAYRGIVGLRDTANEVRKARKQNILVLGVFTGKESELEAERLIYGND
                     FIYSRDITRFSDVVSMYLKKIIRN"
     gene            complement(10189..11118)
                     /locus_tag="ACGI59_003901"
     CDS             complement(10189..11118)
                     /locus_tag="ACGI59_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFG9741604.1"
                     /translation="MDIIDNLRVQGVDEKLIEDVLYFRNYYGLEKDLEYRVTKSKTYF
                     YGKDILSMCIAAILEEENILLSGPKATGKNLLADNLGEIFNRPQWNTSFHINTDSSTL
                     IGTDTFIDNEVKLRRGSVYECAINGGFGVFDEINMAKNDAIVVVHSALDYRRIIDVPG
                     YERVNLHPATRFIGTMNYEYAGTKELNEALVSRFMVIDIPPIEEDKLMMILKNEFSDA
                     DEEKLIHFAGIFLDLQLKSQNGEISSKAIDLRGLMASLKTIRRGLKPTLAINMGLTGK
                     TFDVYEKEMVGDVIKTRIPNKWESIDVFPISHI"
     gene            11339..11524
                     /locus_tag="ACGI59_003902"
     CDS             11339..11524
                     /locus_tag="ACGI59_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFG9741605.1"
                     /translation="MKYGCPEVAKKWGISERRVQKLCEESRIPGVSKLGYMWLIPKNA
                     EKPVDGRTKRRKEVQYE"
     gene            11517..12218
                     /locus_tag="ACGI59_003903"
     CDS             11517..12218
                     /locus_tag="ACGI59_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFG9741606.1"
                     /translation="MNSVLIIDDDKELCALMKKCVEQENLSAITAHCGAEGLHLVDKN
                     RHTLSLVILDIMMPDIDGFQVLKKIRETSNIPVIMLTAKNDEDDKVSGLRLGADDYLT
                     KPFSINELMARVNSLIRRYTMLNPAFITDTDCIIINGMIIDKTNRIVSVNNIPVQLTS
                     KEFDLLAFLASNKGRVFTKKQIYTQVWEEEYAFDDSNIMSFISKLRKKIEPDPNHPFY
                     ILTVRGVGYRFNKEA"
     gene            12220..13143
                     /locus_tag="ACGI59_003904"
     CDS             12220..13143
                     /locus_tag="ACGI59_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFG9741607.1"
                     /translation="MNTSVYLLLILVLSLIIIAYLVSVLLRVRIQLTLIKDALEDIKN
                     GNLNRRILTDENDITKQICYDINEIAINSQFQLIQQKQSEQAYKQLMTSLSHDVKTPL
                     ASLVGYLEAVESKIVVGEEKDEYIHVASNKAHYLKNFVENLFEWVKLDSKEQVFHFDI
                     FDLNELSRNIISDWIPVLESSHFEYEFDIPEIEYFLRIDANAYTRIINNLLQNIITHS
                     SGNKMTLRIFENKEQAQIIITDNGKGISSDNLPHIFERLYQCDHSRASKGNGLGLAIA
                     KELINAHKGNITANSTPGMGTEFTILLPKAL"
     gene            13300..14223
                     /locus_tag="ACGI59_003905"
     CDS             13300..14223
                     /locus_tag="ACGI59_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019639192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9741608.1"
                     /translation="MNDFVIETKQLTKIYGNQTVVDKVNLHAKKGRIYGLLGRNGAGK
                     TTIMKMILGLTSITYGEVDVFGENIKGREKQVYPRIGAIIETPGFYPNLTGTENLEIF
                     AKLRGTAAPNSVKRALEIVGLPYKNKKLFSEYSLGMKQRLGIANAILHDPELLVLDEP
                     TNGLDPIGIAEMRKFIRDLSVEHGKTILISSHILSEISLLADDIGIIDHGVLLEESSM
                     SELEKKNSKYILLQVSDIPKTSLILERQFNTKDYSVQDDYTLHLYNTNLDMGEINKAL
                     VMQNVTVISSQLCNDTLEDYFKKITGGEGIA"
     gene            14216..14953
                     /locus_tag="ACGI59_003906"
     CDS             14216..14953
                     /locus_tag="ACGI59_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9741609.1"
                     /translation="MLKLIQTEFLKLRRRKLIWLMLLSALIMPFFALLYFNYFGKTGV
                     EPIKFYKWSAFGYTLWIILPVVLGIFCTMLIHEENQYDMLKQLWIVPISKMGYFFSKF
                     FVVLIYSICFMLITAVASVLFSVLSGYVVFSWASILYLLKKCLEIGVITAFVMLPILA
                     IATSQKGYILPICITLVYAFSGFVIMTINMYLHPLSSMGVIIMRNKDIPGLIFSQEIN
                     VPLAFLCICIWSIVSVLFANTALGRRK"
BASE COUNT         4759 a         2640 c         1854 g         5711 t
ORIGIN      
        1 atcatacaat atatagattt ccacaataca aataaattaa aaagtgaaat gtatttgtct
       61 aaaaacataa taacatttca ctttatcctc atttttaagt aatatatatt tttaattttt
      121 atttataact aaatctagtt tttatataaa ttttagtttt tttctacatc tgagtaatct
      181 attccaaaag tttctacagt aacagatttc ataacttgtg gagtttgtgg cttatcagct
      241 gaatctgttc taactgatgc tatttcatca actgtatcca taccttctat cactctacca
      301 aaacctgcat actgaccatc taagtgtggt gagtttttat gcattatgaa aaattgagaa
      361 ccagctgaat taggtgccat agttctagcc attgatatga ctcctctttc atgttttaaa
      421 ttgttagtaa atccattacc actaaattca ccttttattg agtgacctgg accacccatt
      481 cctgtaccat ttgggcatcc tccttgtatc ataaatcctg gtattactct atgaaatatt
      541 attccattgt aatatccttc atttactaat gatacaaagt ttttaactgt atttggagct
      601 atatttggat ataactcaat tttgatttct tttccatttt ccatttctat agttactata
      661 ggatttttat tttccataat ttattacctt ccttttcatt agttaaatta cataattaca
      721 tttattatat tatactctaa attaaagtct atcaagtata ttttttccta tttcatatcc
      781 ttcttgataa atagttttac aaaactcatc atcaaagtta agcgtgtctt taggagcaaa
      841 ttgagtatta ggtctaacta ctattatttt atcttcatca tatacttttt taatatcttc
      901 tggcaactca taattatatt ttgtggatat taaaataatt ttatccacat tatccccaat
      961 taatggttca attaatgcac tcctaattaa acctccatct aatttgaaac catcataaat
     1021 tccttcttgc acatctttca caaattgttc tttaaatcca agagttgcgt ttggatttga
     1081 tggtaacaac ccactgtatg aaatgctgat aatggcttca ttacgaggca tttttgatac
     1141 gtcaacaatc ttttctgata ctactttatt atcaacttca acataattaa cataaaagtt
     1201 catttcttct atattactat catctagttc ccttaggttg tctatagcag gagaattatc
     1261 cacagtatta tttactattt ttacatttcc attctggata tcttttatat ttgtccacat
     1321 tttctccaag ttatcaacat tccctgtgta caaataatat ccatttattg ctcctattga
     1381 tgtccctgaa atcgaataaa atttatttat tcctccatca tacaaggctt taactactcc
     1441 tgcttgataa gccccttttg ctcctccacc ttctaaacat aatcctaatt tcatagtctc
     1501 atctccaaat tgtttatctt atatttatat ttcaaaaaac ttactaaatg ttttatatat
     1561 actatagtgg tcctcaacat ttcctaattc tcttatagaa tgcattgcta aaattggact
     1621 tcccacatcc acagatggta tatctatatg agtagaagat attggaccta tagtacttcc
     1681 tcctctttca tctgacctat taacaaattc ttgatactca acattagctt ctctacatat
     1741 atttttataa atagctatag aatatccatc actagtatat gcctgactag catttatctt
     1801 tataaccgga ccttttccca ttattggttt attagttggg tcatgctttt ctggaatgtt
     1861 tggatgaata gcatgagcta aatctgatga tatcataaaa gatgaataaa tagactcaaa
     1921 gaatccctct ctatccttac ccattgatat gcaaattctc tccaatatat ttaacaacat
     1981 gtttgaatct gcaccttgtt tagtagagct accaacttct tcattgtcaa atacagccat
     2041 tacatttaca ccatgttgac ctttagtatt aacaagagca ttcaaactag catgtaccat
     2101 tgcaagatta tcttgtctcc caatagatat aaattcatca ttagccccta ttaaacttcc
     2161 tttttcaaat tcatataaaa ataaatcaaa atctataata tcgtccatat ttacatctaa
     2221 gttagatgct atttgcttta taagaaaatt atctttttct aatgtatcat ttaacatccc
     2281 aactaaaggt aacatatctt tttgcttatt atatgagtga ccatcattta tacttctatt
     2341 taaatgtatt gctagatttg gtattataca aattggcttt ttaaagtcta taagcctttc
     2401 atttggtttt aatatactat caccttttaa aactactcta ccagctatcc caagaggtct
     2461 atccaaccat gtacttaata ttgctcctcc atagcattct gtatttaact tcaaataaga
     2521 tttttcagct tctatttctg catttggttt aatactaaat gtaggagaat ctgaatgaga
     2581 gcctattatt ctaaatccat ctctttctat accttctgaa tttactacaa aagctactat
     2641 agctgataaa ttcttagtaa cataatactt tccaccaact cttagattcc atctttgatt
     2701 caaaactaat tcttcaaatc catttttatc taagatttcc ttagtactct cgactgcatg
     2761 aaaagacgac ggactatcat atatgaagtc tattaagttt ttagcaaagt ttatactatc
     2821 cattcctcat ttacctccat tataaaatat ttagtgttgt atattcttta tttatttttt
     2881 taattccctg ttggtcaaaa gctatagtaa catctgtacc aattatagaa accactgtcc
     2941 caaccccaaa tttaggatga tgtaccttag aaccaagttt tatatcatct ggattagttt
     3001 ctttattaga atcttttatt gtagcattaa ctttatctgc aactttagat ttgcttatag
     3061 tactcatata ctttttagta tatttatcta acatattata acttgcttta gaatagctta
     3121 gttctttttt tactttgtac aatctttcta tacattcttc tggcagttct tccataaatc
     3181 ttgaagctat tgcaacattt gttttaccat ataatgttct cttctctgtc aatgacatat
     3241 atagctcttc tttagctcta gttataccaa cataacaaag tcttctctct tcttctattt
     3301 gtgaatcact catagatttt atagctcttg atataggaaa taacccttct tccatacctg
     3361 ttaaaaacac aacagggaac tctaaacctt tggatgtatg aattgtcata agcgaaaccc
     3421 tatcattgtc ttcttcatca tttgattcag atgtaagtgc tatacttgtt aggaatgtct
     3481 ctaagtcttt attttcataa gtatcatcat tactactttc aaactccaaa gctatagaaa
     3541 taaattctct caaattatca attctgtctt gagcttcttc acctttgcct gttaaatctt
     3601 tttcattttt atttcttatc tctacaagct cgtccatata tcctgtagta tccaagactt
     3661 tttctataag tttgctaaca ggataaactt cttttatagt tctaagagtc cctataatat
     3721 caacaaatcc acttatactt gcccttgcct tagtagatat atctgaattt gtttctatat
     3781 caattaatac tgagtatata ctttcctgtt ttaagttagc tctatcttca attttttcta
     3841 tagttctaag ccctatacct cttctaggaa catttataat cctttttata gatatatcat
     3901 cttgtggatt ttgaataact cttaaatacg ccaacaaatc ctttatttct tttctctcat
     3961 agaattttgt accaccatat atattgtatg gaatttgact tctatttaaa gcgtcctcta
     4021 caggtcttgc ctgtgcattt gctctataaa gtactgcaaa atccttataa ggtctatttt
     4081 cttttctagc tatttgtgca attgaatcag caatgaaatc agcctcttct atttcactac
     4141 cagttaattg tatctttata agttctcctt cttttttttc actccaaagc ttttttcttt
     4201 ttctttctat attattagat ataactttat tagctgcatc taatataact tgtgtagacc
     4261 tataattttg ttccaacttt acaacatgta cattgtcata atctttctca aattcaagta
     4321 tgtttcttat atctgcacct ctccatccat aaatactttg gtcatcatct ccaacaacac
     4381 aaatattttg atgttctctt gctaataatt ttatcaattc atattgagct ttacttgtat
     4441 cttggtactc atctaccatt atgtatctaa atctacttct gtaatatgct aacacttcat
     4501 cattagcctt gaaaagttct acagttttaa gtattaaatc atcaaagtca agtgcactat
     4561 ttctttttaa acggtcttga tatagcgcat aaatatctgc aatcttaacc attctattat
     4621 ctgccatatt gatatcttta aactgctttg gagtataaag tttgtctttt gctcctgaaa
     4681 ttgcacttat aactgctttt ggttcaaaca ctttatcact taaatttaat tcttttagac
     4741 agtctttaac caatgtaact tggtctgctg aatcataaat tacaaaactt ctattgtaac
     4801 caatcttatt tatatctttt cttagtatac gaacacagca agaatggaat gtacttatcc
     4861 acatatcctt agtttcagga cctatatttt gttcaactct ctctctcatt tcatttgcag
     4921 ctttgtttgt aaatgttata gctaatatat ttggtgcttg tacaccttta tcttctataa
     4981 gatatgctat tcttgttgtt aataccttag ttttacctga accagcacca gccagtataa
     5041 gtactggacc ttcagtcttt tctactgctt ctctttgagc aggatttaat gtatctaaat
     5101 tcatatttct cctcctgtta acagccataa atatatttta atctaaaata tgaattactt
     5161 caaattataa ttatgtatta tattaataga ttatttccaa tatattacaa ttaaataaca
     5221 tttttccata taatttattt cattgtttaa taaaaaaaat aaccttatga tttggggaat
     5281 cataaggcac ctttaagggg tctaggttaa gggttattat atataaatct atttttttaa
     5341 aataggttta gggcttaatt atttatttgt attaagttct ctttccttcc aatacttata
     5401 caaatcttct tctttcttaa ataagggtaa attcatattt ctatgtactt ttactaacca
     5461 tggggattct aggccagctt gtataatatt attttcattt ataaacacat tttctgcttt
     5521 tccttcatca atcaaaatcc ccttatccaa tacatatatg taatcacata tttcatacat
     5581 taaattcata tcatgactgg atattactat ttttatatta tttttattta atccctttat
     5641 tatatctaca atagacctag tacttactgg gtctaaacca gctgtaggtt catcaagtaa
     5701 tacaatttca ttttccattg ctataactga tgctattgcc actctttttt tctgtccata
     5761 gctaagaaaa tgtactggcc tgtctataaa atctattcca ttaacagcta ctagagcttt
     5821 gtttattctc tctttaattg ttttttcatc cataccaatg tttctcattg caaaggctat
     5881 atcatcatat acttttgaat aaaaaatttg tttttctggg tcttggaaga caatacctac
     5941 attttttcta aggttgtata aacttctctt atcatatttt aacttttctt ctttaaaaag
     6001 aatttctcca gatgtaggct ttaatatacc cattaaattc ataaataatg ttgattttcc
     6061 tgaaccattt gaacctatta tacctataac atttcccttt gaaaaatcca tattaatgtt
     6121 taatagtgca ttggtatttt tttcatattg atacgttaaa ttatttattt taaacatcat
     6181 tatcccctac tatgtgaaat ttaccatcat acaattttat gtctaatgat atacacatat
     6241 catcatacct tgtcataagt cttttgtaca gcatacttcc aagaatacct aaggatttat
     6301 atgaagtttt taaatttata tatccaaatc ttagctcttg tgacttgtat atttctgaaa
     6361 attcttctaa aaatatgaat ataaacctat atattaacat tgataactcg acaaaagtgt
     6421 caggtaaatg aagtttttta aaaaagaata ttaattgatt aaaaggtgtt gtaagtatac
     6481 aaaaatacac acaagtcaaa caagacatcg ctctaaataa cacatgaata gacatgttaa
     6541 tagacgcctt tgatattcct acattaaaac taaaaaattc aaaactatat aataaatctg
     6601 ctttattaaa agatatattt acaagagtta ttcctatact aagaaataaa aagtacattg
     6661 gtattctaag taattttaaa taatttttta aatctattcc agccacacaa acaaccaaaa
     6721 tactcattaa aatcataatt ccacttaaaa taaatatatt ttgtattacc atagaagcaa
     6781 tcagaaaaat tactcctatg gctacttttt tgtttggatt tactttactc aatctatttg
     6841 tatatgcata tttatcaatt ataagcatga ttaacttctc tttccttttt gaagacctaa
     6901 tatgtatcct attacaccag cccctaaagc tgcttgtgca gagaataata aactttctat
     6961 ttctccactt ggtggctcat atggactgct aaaccatggt ttgtaatttg gatttatctt
     7021 agtaatttca ctttcagctt gaccatctgc tcctccatat tcagcacctg agttgacaag
     7081 taatgggaat ataataagag ctactactaa taaaagtaat aatatgttag tttttgtttt
     7141 tgttttagca ctcattagct ttaactcctt tatcattatt ttctagtatg cttttttctc
     7201 cgttttctgt aattaaatta tatacaatta cagtaagtat accttctgct attgctattg
     7261 gtatttgagt cataaagaaa attcctaaaa atttaattgc tgaagccatt accccacctg
     7321 atgggtctgg gaaagccagt gctaattgta ttgaagttat tgtatatgtg gctaagtctc
     7381 ctattgctgc tgcaagaaat actggaatag cattatttcc atcttttttc tttaaagcct
     7441 taaatattag gaatgatacg attggcccta ttatcgccat tgaaaaagca ttagctccta
     7501 aagtagttat tcctccatgt gctaataaca atgcttggaa tattaaaact atagttccaa
     7561 gaacaaacat tacgcttggt ccaaacatta ttgctccaag acctacacca gtaggatgtg
     7621 aacaacttcc tgttactgaa ggtattttta gtgctgataa tacaaacaca aatgctccac
     7681 ataaagctaa taatactttc tttttagggt cttgctttac tattttacct attgatttta
     7741 atccgaataa gaaaaatggt ataaatataa ctccccatgc tatagaccat tttactggta
     7801 agtaaccttc cattatatgc attgcatttg atgtaactgg agttaaaatg attaacataa
     7861 gtgcagctat aacccctaat ttaatattct gtttcatctt agtatccctc tctccctttc
     7921 taataattaa aacataataa tacatgtctg atttatttaa taaaataaaa aagcctaact
     7981 cgatggttag gcttctaatt ttccacaatc taaatacaaa cacataaaac aaacatatct
     8041 gtataattat tataagaaac tttctccatc cccgaagaaa ttactttaat attttaggca
     8101 ggtctcctga cttagcttca tcctactcct tttccttccc atgagatata aaaaatatca
     8161 tcatagtggc taacaaaagt ttcgtctgca tcacagtagc gggggctgta aaggaattta
     8221 acctttttcc ctattaatct tgcttaaaga acctataata ttatatattt aatttttaaa
     8281 ttattcttaa atcattttct atataaatca ttgtatacct tttctgtgaa aatttctaca
     8341 ttttttataa attttattca tttttatatt tttatgtata taaattcttt tttgtaagtt
     8401 tattaatttc taattatctt ctttaaatac attgaaacta catcagaaaa tctagttata
     8461 tctcttgaat atataaaatc atttccatat attaatcttt cagcttcaag ttctgattct
     8521 tttcctgtaa atactcctaa tactaatata ttttgctttc tagcttttct cacttcattg
     8581 gctgtatctc tcaaaccaac tataccccta taagcctttt ctcctcttaa tgttctctca
     8641 ctactttttc ctatctttac atcatttggt cttccatcgc tgagaactat caggatttta
     8701 ttttcttcat ctctatttat tagaccacta catatagatt ttattgccaa accatctcta
     8761 ttattaccag cacaaaaata ttcaaatata ttttcactat tttttatact atcatcataa
     8821 tctttaaatc tttttaatat tgtatagtct aaaaaactag aaaatcccat cactctattt
     8881 ggtatgccaa cttctgttag agcatttgca attatgtaag cttgaattgc cacatttccc
     8941 tgatttctac tttgagagcc acttgcatcc aataatatat caataacgta tttccctttt
     9001 tcattttcta tatctttata aaatatttta ttattattac ttctaccaac tctccaagct
     9061 ctattggcta atatagtacc acaatcagaa tatactctac ttttagcatt ttcttctatt
     9121 aaaattcttg acagactttc ttttaatctc tttatttgct gtctatacat tctagcatgt
     9181 tctctaaatt ttcctaaatt attttctttt tgtcttgtta catattttag ttgaaataca
     9241 ttattacact cacttctaag aactccatct gtaaagtgta ttctacaacc ttcatgaaca
     9301 tttctacaat tttgtgtctc aattttttta atttcacctt tactcaaata agtttttcca
     9361 taataatgtt ctatttttga atatatctta tttgcagtgt cttcatcaac atatataact
     9421 cggtctgctg aattgctaca tttagaatca tcaaaatccc ctacaccttc tacaagcatg
     9481 gatgaagata ttgaatctat atcactttca ataatatttt cttcttcatc actataaagt
     9541 tcttcataca tgaaatcagc aaatgtatca aaatttactt ctacactttc tacattattt
     9601 tcatcaataa gactattttc ttctccattt aatatgtatt caaaataagt taaatatata
     9661 acatctaatg cttttaaaat ctccataata tcttgattgg tatctatatt tcttatatca
     9721 ttaactattc ttctagtcat actatctaat gctgggtgct tgtccatact ataaagtaca
     9781 aatgtgtatt taattttatc cataatatta tcattgtgta ttttggtaaa acgtgaaaca
     9841 atatctttat aagcattcct tcttatgtct tctatccctg gcctgtcttt aatgaccttt
     9901 tcttcaacta ttgtatttaa tataacttga attagaccta gtataacatc tttgttgtat
     9961 ccttttctta ttctatttgt aagatatttt ttaactatat cccaatctac atactttctt
    10021 ctagcacctg caattattgc gaaatataga cctatttctt ttgaagagta atctttttct
    10081 gatatatcta tactttcatt gtaatcacca gctattgtcc atgataggtt attagctcta
    10141 ttttcaaatt cataatcatc atatatccat ttcataatct cacctatatt aaatatgact
    10201 tattggaaac acatctatac tctcccattt attaggtatt ctagttttaa ttacatctcc
    10261 aaccatttct ttctcatata catcaaaagt ctttcctgta agtcccatat taattgcaag
    10321 agttggtttt aatcctcttc ttatagtctt aagagatgcc ataagaccac gtaaatcaat
    10381 agctttacta gatatctctc cattttgaga cttcaattgt aaatctaaaa atattcctgc
    10441 aaagtgtatt aatttttctt catcagcatc tgaaaattca ttttttaaaa tcatcattaa
    10501 cttatcttct tctataggag gaatatctat taccataaat ctagatacca gtgcttcatt
    10561 aagttctttt gttcctgcat attcataatt catagtccca ataaatcttg ttgctggatg
    10621 tagattgact ctctcatacc caggtacatc aattattctt ctatagtcta aagctgaatg
    10681 taccacaact atagcatcat tttttgccat attaatttca tcaaacaccc caaaacctcc
    10741 attaatagca cactcataaa ctgagcctct tctaagttta acttcattgt ctataaatgt
    10801 atctgtacct ataagtgttg aactgtctgt atttatatga aatgatgtat tccactgagg
    10861 tctattaaat atttccccta gattatcagc aagtaagttt tttcctgttg cctttggtcc
    10921 cgataataat atattttctt cttctaaaat agctgctata cacatagaca agatatcttt
    10981 accataaaaa taagttttag actttgttac tctgtattct aaatcttttt ctaaaccata
    11041 ataatttcta aaatacagta catcttcaat aagtttttca tctacccctt gcacccttag
    11101 attatctatt atatccatat ttttacccct tttatataaa tagtatttgt ttcatttctt
    11161 atcaccattt tagcataaaa ggatataagc taaaattgat tttttaatct ccccatttta
    11221 agtaaaacca tgcaccattt tatcactaca aaattaagag tactacttac tgatatttct
    11281 tgattttgtt cgtctatacg tatataatat atgctgataa attatgagga aaggatgatt
    11341 gaaatatgga tgcccagaag tagcaaaaaa atggggtatt tctgaaagaa gagtacaaaa
    11401 actatgtgaa gaaagtcgca taccaggagt ttcaaaactc gggtatatgt ggctgatacc
    11461 aaagaacgca gaaaagcctg ttgatggtag aacaaaaaga agaaaggaag tacaatatga
    11521 atagcgtttt gattatagat gatgacaaag aactttgtgc tcttatgaaa aaatgtgttg
    11581 agcaagaaaa tttatctgct attactgcac attgtggtgc agaaggtcta catttggtcg
    11641 ataaaaatag acacacactc tctcttgtaa ttttggacat aatgatgcca gatatagacg
    11701 gttttcaagt tctgaaaaaa attagagaaa caagtaatat tccagtaatt atgcttactg
    11761 ctaaaaatga cgaggatgat aaagtttccg gactacgttt aggtgctgac gattacctaa
    11821 caaaaccgtt cagcataaac gaacttatgg ctcgtgtcaa ttctcttatt cgtcggtata
    11881 ctatgttaaa ccctgctttt ataacagaca cagattgcat aataataaat ggcatgatta
    11941 ttgacaagac aaaccgaatt gtttctgtca ataatattcc tgttcaactt acaagcaaag
    12001 aatttgattt acttgctttt ctggcttcta ataagggacg ggtgtttacc aaaaaacaga
    12061 tttatacaca agtatgggaa gaagaatatg cttttgacga tagtaacatc atgtctttca
    12121 tcagtaaatt gagaaaaaag attgaaccag accccaacca tccattttat attttgacag
    12181 ttcgtggtgt cggttatcgt tttaataagg aggcttaaca tgaatacaag tgtctatctg
    12241 cttttgatat tggttcttag cctgattatt atagcgtacc ttgtttctgt cctgctacgt
    12301 gtccgaatac aacttacact tataaaagat gctttagagg atataaaaaa tggaaacctt
    12361 aatcgacgta ttttaacaga tgaaaacgac attacaaaac aaatttgcta tgatattaac
    12421 gaaatagcaa tcaatagcca atttcaactt attcagcaaa aacaatcaga acaagcctac
    12481 aaacagctaa tgacaagtct ctcccatgat gtaaaaactc cacttgcttc tttggtcggt
    12541 tatttggaag cagtagaaag taaaattgtt gtaggagagg aaaaagacga gtatatccat
    12601 gttgcgtcta ataaggctca ttacttaaag aattttgtag aaaatctatt tgaatgggta
    12661 aaattggatt ctaaggaaca agtttttcat tttgatattt ttgacttgaa tgaattatcc
    12721 cgtaatatta tatctgattg gatacctgtt ttagaaagca gtcattttga atatgaattt
    12781 gatataccag aaatagagta cttcttacgt atagacgcaa atgcatatac ccgtatcatt
    12841 aataacctat tacaaaatat tattacccat agtagtggca acaaaatgac attgagaatt
    12901 tttgaaaaca aagagcaagc tcaaattata ataacagata atgggaaagg tatatcttca
    12961 gacaatctcc cacatatttt tgaaaggttg taccaatgcg accattcacg agcttcaaag
    13021 ggaaatggtt taggcttggc gattgcaaag gaacttatta acgctcataa gggaaatatt
    13081 accgccaaca gcactcccgg tatgggaaca gaatttacaa tattgcttcc taaagccctg
    13141 taaaagtacg aggctttctt actctaaaac aagataaaag caagattatg gcaaggtttt
    13201 ggcaaggttc atgttttata attctaattg tagttgattt tgacgttgta tcaactatct
    13261 tttgtagaaa caaatttatt aaaagtgagg aaattatata tgaatgattt tgtgattgaa
    13321 acaaagcaac ttacaaaaat ttatgggaat caaacagtag ttgacaaagt caatctccat
    13381 gcaaaaaaag gccgaatata tggtctgtta ggacgcaacg gagctgggaa aaccacaatt
    13441 atgaaaatga ttttaggatt gacttcaatc acgtatggcg aggtagatgt atttggagag
    13501 aatattaagg gacgtgaaaa acaagtatat ccccgtatcg gtgcaattat cgaaacgcca
    13561 ggattttatc ctaatctgac aggaacggag aacttggaga tttttgcaaa actgcgtggt
    13621 acagccgccc ctaattctgt aaaaagagca ttggaaattg taggcttacc ttataaaaat
    13681 aaaaagctgt ttagtgaata ttcacttggc atgaaacagc gacttggtat tgccaacgct
    13741 atcctgcatg acccagaact gttagtttta gatgagccaa ccaatggtct tgaccctatt
    13801 ggtattgcgg agatgagaaa gtttattagg gacttgagcg ttgagcatgg aaagacaatt
    13861 cttatttcca gtcatattct ttcagaaatc tcattgttgg ctgatgatat tggaattatc
    13921 gaccacggtg ttcttttgga agaaagtagt atgagtgaac tagaaaagaa aaacagtaaa
    13981 tatatcctgc tacaagtttc agatattccg aagacatcac ttattttaga gcgtcaattt
    14041 aacacgaagg attattccgt tcaagatgac tacacgttac acctttataa cactaatctg
    14101 gatatgggtg aaatcaataa agcacttgta atgcagaatg taactgttat tagttctcaa
    14161 ctttgtaatg atactttaga ggactacttt aaaaaaatca caggaggaga gggcattgct
    14221 taaacttatt caaacagaat ttcttaaact gcgtcgtagg aaacttattt ggctgatgtt
    14281 gttgtctgct ctcattatgc cgtttttcgc actactatac ttcaattatt ttggtaaaac
    14341 aggtgttgag ccaataaagt tttataaatg gtctgcattt ggttatacac tttggattat
    14401 tttgcctgtt gttttgggta tattttgcac catgcttata cacgaagaaa accagtatga
    14461 tatgctcaaa cagctttgga ttgtacctat tagtaaaatg ggatactttt ttagtaaatt
    14521 tttcgttgtt ttgatttact ctatttgttt tatgctgatt acagccgttg cttctgtgtt
    14581 atttagtgta ctttctggat atgtcgtatt tagctgggca agcattttat atttgttgaa
    14641 aaaatgttta gaaattggtg taatcactgc ttttgtaatg ttgccgattt tggcaatcgc
    14701 tacatctcaa aaaggataca ttctcccaat ttgtatcaca ttggtatatg ccttttccgg
    14761 ttttgttata atgacaataa atatgtattt acacccatta tccagtatgg gagttattat
    14821 catgagaaat aaagacattc caggtcttat attttcacag gaaattaatg tcccattagc
    14881 atttctttgt atttgtattt ggagtattgt atcagtattg tttgcaaaca ctgcactagg
    14941 aagaagaaag tgaggtgtag ataa
//
LOCUS       DAYAPA010000302        14345 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.guided.114, whole genome shotgun sequence.
ACCESSION   DAYAPA010000302 DAYAPA010000000
VERSION     DAYAPA010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14345
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.guided.114"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            246..863
                     /locus_tag="ACGI59_003907"
     CDS             246..863
                     /locus_tag="ACGI59_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFG9741610.1"
                     /translation="MCKKTYLLVVSMLIAGLLTACSGSPTKDSGQAQDNTKKEANASN
                     NALKDEKNNENLMEQDFKVPYTDAINIFKDKYKDADIVDLSLERDLNKFVYTVEGVDD
                     NNEYKMKIDANTKDVLEDKTEKLDSEDLNGVARKEKLDLNDIMTPQQAMEIALKEQNG
                     IVKEWSLDKDLDVTFYKIRIDKDKNEYDIKVDSKKGTVLKVEKED"
     gene            971..3502
                     /locus_tag="ACGI59_003908"
     CDS             971..3502
                     /locus_tag="ACGI59_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFG9741611.1"
                     /translation="MSLKSLKIKENLYWVGSLDPDLRVFDIIMYTPYGTTYNSYVLKG
                     TEKTVLFETVKDKHFDNYIERLNDLNIDFEKIDYIVVSHTEPDHAGSVEKLLDLAKNA
                     KVVASETAIKYLKEIVNKDFEYVAVTDGDTLSIGDKTLEFFSVPMLHWPDTIYTYIKE
                     DKTLVTCDSFGSHYSNDKIVNTLDENEEKDYLDALRYYYDCIMGPFKPSMVTAIEKIK
                     DLDIDTVCPGHGPVLTENPRKIIDLYYNWSVNEQIKLEKEVTICYVSAHGYTKIMAEA
                     IKAYIEKNSNYKVNLFDVIEHKQEDILAKIAVSQGVLFGTPTILGDALKPIWDILISL
                     NPVLHGGKVASVFGSYGWSGEGIENAMERISQLRMTAVKPFAVNFKPSNEEIDKLYSY
                     TGKFLDKLNSTFGSKKKTKKFKCVICNEVFEGDSAPSVCPVCGAKEDQFIEVEEDEVT
                     FRKDTDEYFVIVGNGAAGFYAADAIRKRNKTCKITMISNEDELTYYRPALSDGINEEL
                     GSDFYMEDKDWYDKNNIVVILGTNVDKLDEVNKTIIVNDGAIKFDKLVIATGSRNFIP
                     PIKGHDLENVFTLRNIKDLYSVKEALEKSKKVVVIGGGLLGLEAAWEFRLKGLEVVVV
                     EAMDSILSKQLDKEGSKILEQCVRDTGIDVRLGVAVDGIEGDGKAQKVVFKDGDSVDC
                     DMVVFSIGVRANTQMVQDTSVKIDRGIVVDKTLQTNVKDIYACGDVAQVGNISLAIWP
                     SSVEMGKIAGANASGDNLTFESEVYPVSLDAMNVKVFSIGNIQNFDKEISSKDEGQRI
                     YKKLFMKDGSLVGAILINDLSCTVKLIRLISEKGDFEDIMKADIL"
     gene            3936..4637
                     /locus_tag="ACGI59_003909"
     CDS             3936..4637
                     /locus_tag="ACGI59_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-G-Cd"
                     /protein_id="HFG9741612.1"
                     /translation="MNENILIVDDEKEIADLIEVYLKNEGFTVYKFYTGKEALECIES
                     KNLDMAILDIMLPDIDGFHICQKIREKYYYPIIMLTAKIEDIDKIMGLTIGADDYITK
                     PFNPLEVVARVKTQLRRYMRYNNSYEQQSIIVNEYDIKGLIINKETHKCSLFGKEVAL
                     TPIEFSILWYLCEHQGKVVPSEELFEAVWGEKYLDNNNTVMAHIGRLREKIHEPSKNP
                     KFIKTVWGVGYTIEK"
     gene            4687..5769
                     /gene="vanS"
                     /locus_tag="ACGI59_003910"
     CDS             4687..5769
                     /gene="vanS"
                     /locus_tag="ACGI59_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS"
                     /protein_id="HFG9741613.1"
                     /translation="MRTALMAFIAIIFIWILYSFILYGKFSIWIVSFFENAFRMEYKA
                     ALNLYQQIFRNSMELFMILAMVIVFFIIFRVYLNWFTKYFSEINQGIDSLIKEDVGEV
                     ALSPELLAIEKKINSIKHILEQRKFETQMAEQRKNELIVYLAHDLKTPLTSVIGYLTL
                     LRDESQISEELRKKYLSISLDKAERLEDLINEFFEITRFNLSNISLEYSTVSLTRMLE
                     QLTYEFKPTLMSKNLKYTLNVTPNMMIRCDVNKMQRVFDNLLRNAVYYSFEDTTIEIT
                     AVQEDDYIKLKFINHSNTIPEEKLERIFEQFYRLDTSRNSNSGRAGLGLAIAKEIVEL
                     HNGTITAHSENNIIIFKVIIPIVGNL"
     gene            6030..7130
                     /gene="vanG"
                     /locus_tag="ACGI59_003911"
     CDS             6030..7130
                     /gene="vanG"
                     /locus_tag="ACGI59_003911"
                     /EC_number="6.3.2.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-serine ligase VanG"
                     /protein_id="HFG9741614.1"
                     /translation="MNNKKIAVIFGGNSTEYDVSLQSAFAVLENLNTEKYDIIPIGIT
                     REGNWYHYIGDYNNIQNNTWFERDTDIIPVIVSQNRVKNGIIEFLIPDAVSQNHIPNS
                     AIESCENDIKYTKLDFVFPVLHGKNGEDGTVQGLFELAGIQVIGCGTLSSALCMDKDK
                     SHKLVHSEGIKVPKSILLKYSDKFNVAELSKDLTYPLFIKPVRAGSSFGISKIYKKEE
                     LQVAVDLAFEHDNEVIIEENIDGFEVGCAVLGNDDLLVGRVDEIELSKGFFDYTEKYT
                     LKSSKIHMPARIDLDCEKRIQETAKIIYRTLGCSGFARVDMFLTPSGEIIFNEVNTIP
                     GFTSHSRYPNMMKGIGLSFEEILDKIIEVYIK"
     gene            7127..7933
                     /locus_tag="ACGI59_003912"
     CDS             7127..7933
                     /locus_tag="ACGI59_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HFG9741615.1"
                     /translation="MMNNILLDKNKEDKMTNTILLTKDKVYDGNLILVNAFLPVKTSE
                     DIDLIPVDTRFPSILMKREATNILQNILKSICGINEIVPVSGYRTAEEQQDIYSSSLR
                     DNGKDFTKKFVALPNHSEHQTGLAIDLGLKKEVIDFICPEFPYNGICNEFRKAALHYG
                     FIERYQCKKEEITGISQEPWHFRYVGYPHSEIINKMNLALEEYIEYIKRFSYTNCLKI
                     EDKEKIIEIFYVPIHTKEEVRISIPEQCVYQISGNNKDGFIVTFWRNRKD"
     gene            7951..10089
                     /gene="vanT"
                     /locus_tag="ACGI59_003913"
     CDS             7951..10089
                     /gene="vanT"
                     /locus_tag="ACGI59_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine racemase VanT catalytic subunit"
                     /protein_id="HFG9741616.1"
                     /translation="MIRNKSYAGLDYFRIIAALLIIAIHTSPLLSYSKMADFVLTSII
                     ARIAVPFFFMLSGFFLISTYNYNCNKLKSFLKNVLFIYGIAIVIYIPLNIYTGYFSMN
                     NLVPNIIKDIVFDGTLYHLWYLPASIIGASIAYFLVKRLGLKYSLVITIVIYVIGMFG
                     DSYYGFLEKIPLLKNLYGYIFEVSDYTRNGIFFAPVFFILGGIISNKSNYMSTKNSLI
                     GFLISFLLMLCEGMFLHKLGVQRHDSMYIMLLPCMYFLFTALTFLKGSRARLLRTSSL
                     IIYIIHPMMIVVIRMFSKIFGIQTVLVDNSLIHYIVVSIASFIASIIIILILKPLKKD
                     LKGEHKVNTNRAWIEIDLNNLEHNVKMIQKAMPEDCELMAVVKANAYGHSAFEVATCA
                     NQIGVKAFAVATIDEGIDLRRYGILGEILILGYTNPIRAKELHKYNLTQSVIDLNHAI
                     SLNKQKYNIKSHIKIDTGMHRLGFDVNDVRSILKAFKLKYLDICGIFTHLCVSDSLKD
                     EDVDFTNKQIECFYKLIDFLLKKGIKIPKVHIQSSYGFFNYPNLKCNYVRIGIALYGI
                     LSSPNDTTKLQLDLRPVLSLKSQVILIRKIQKDDSFGYGRVFIASRDSVIAILSIGYA
                     DGIPRNLSCGKSYVIINGCRAAIVGRICMDQLAVDITDIPNVEVGNTAIIIGRDNLSE
                     LSASEVANNSCSISNELLSRVGRRLNVIKK"
     gene            10403..11023
                     /locus_tag="ACGI59_003914"
     CDS             10403..11023
                     /locus_tag="ACGI59_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFG9741617.1"
                     /translation="MQYIQELLDLTQNYWVLATIIGLLSAFIESFIPALPLVAIVTAN
                     AAIQGLFIGCLLSWIGSGLGTTSLFLLISRFNDSKLFNKLRNSKTEKAISWMDKQGFK
                     LLFIAYACPFMPGCLVTIASAFGKKDLKDFVPAMLAGKFVMFIVISYVASDIEGFITS
                     PLKIASFILLVFLSWKIGNRVNKNLENHNYDFHHKKHHNDKDDKMI"
     gene            complement(11189..11758)
                     /locus_tag="ACGI59_003915"
     CDS             complement(11189..11758)
                     /locus_tag="ACGI59_003915"
                     /inference="COORDINATES: protein motif:HMM:NF016214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFG9741618.1"
                     /translation="MFIVTIQEDFYMIKETHERGGYIFALLILPFINNLYLVKYDIAY
                     KIVLIIIYIYFAYLGSLFPDIDMRGSYISKKFMLIYKLFGSRFRHRGFTHSLIALLLI
                     YSFFKSLTIFTNNNIVFSCLSSGFIIGYLSHLCLDLITKEGIELFYPITINISLLPIK
                     TNSKTEKFISKLLNFIVIFLIGYQFYILL"
     gene            complement(11838..12542)
                     /locus_tag="ACGI59_003916"
     CDS             complement(11838..12542)
                     /locus_tag="ACGI59_003916"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HFG9741619.1"
                     /translation="MKKKILIPVIMSLFIISQCITSFAFTPENNKFKVKPLPYAYDAL
                     EPYIDKETMKLHHDKHYQAYVDKLNAALEKYPELYNYSLCELLQNLDSLPKDIATTVR
                     NNAGGAYNHKFFFDIMTPEKTIPSESLKEAIDRDFGSFEKFKQEFQKSALDVFGSGWA
                     WLVATKDGKLSIMTTPNQDSPVSKNLTPIIGLDVWEHAYYLKYQNRRNEYIDNWFNVV
                     NWNGALENYKNLKSQD"
     gene            12715..>14345
                     /gene="asnB"
                     /locus_tag="ACGI59_003917"
     CDS             12715..>14345
                     /gene="asnB"
                     /locus_tag="ACGI59_003917"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HFG9741620.1"
                     /translation="MDGNISIYYKGDISKKEDIKFKIKESVNNTRLSSRIEYKDEEKT
                     FDFERFNVIDAIYGNKPFEKDGKVIVFNGNIYNYHEIKEELIKKGHNFTTNDDIEVLL
                     ASYIEFGKNCVHKIKGMFNFIIYDRENESIFGARDLFGIKPLYYINKENAIIFSSEYK
                     FLLEYMKNLNINERSLQSYFSFQYVLPEDTMIQGIRLIPAGHYFRVENGILSLKRYNK
                     LEFRSSTKFFYTKNHLGNRDVNEDDIRNIVVDSIVTHMEEEKEIGTFLSGGIDSSIIT
                     TVASQINPNIKSFSAGFSVKGYSELEVAKKTADKLGIENIQINITQDEYIKSLPNVIY
                     SLDDPIADPSEVGLYFLIKEAGKHVKVALSGEGADELFGGYNIYKEYSTMKSVVNSPT
                     YIKSILGRVSELMPNIKGRNYLYRATTPLEKRYIGNAKVFENSEVKRFFFKYKEKNIY
                     EYLLSNLYRDAQKNNYDYISKMQHIDVNTWLQGDILQKISKLSTAEQVELRVPFLYKD
                     VFDVAKNLRMEQKINKNNTKVLLREAFREIVPEHVV"
BASE COUNT         5510 a         1521 c         2466 g         4848 t
ORIGIN      
        1 caaaatgaac tttttagttc atgaggcact ctttttgtac gaaataaaat atatttttat
       61 tatttcaaca atgaaaaaga ggcttgtttc cattttgaga cagtataatt tttatgtaac
      121 tttaatttta gtaaatataa agttttaaaa ttgataaaat aggaggaatt tttaatataa
      181 aagggtaaat aaagggttta gagggcataa tatagaataa attaatatat ttaaggaggg
      241 tttttatgtg taaaaaaacg tatttattag tggtaagtat gcttatagcg ggtttattaa
      301 ctgcatgtag tgggagtcca actaaggatt caggacaagc acaagataat acaaaaaagg
      361 aggcaaatgc atcaaataac gctttaaaag atgaaaaaaa taatgaaaat ttaatggaac
      421 aagattttaa agtgccatac acagatgcaa taaacatatt taaagataaa tataaggatg
      481 ctgatatagt agatttaagt cttgagagag atttaaataa atttgtttat actgtggaag
      541 gtgtagatga taataatgaa tataagatga aaattgatgc taatacaaaa gatgtattag
      601 aagataaaac agaaaagttg gattcagaag atttaaatgg agttgctaga aaagaaaaat
      661 tagatttaaa tgacataatg acacctcaac aagctatgga aattgcatta aaagaacaaa
      721 atggaatagt taaagaatgg agtttagaca aagatttaga tgttactttt tataaaataa
      781 gaatagataa agataaaaat gaatatgaca taaaagtaga ttctaaaaag ggaactgtat
      841 tgaaggtaga aaaagaagat tagaagattt aaactatctt taaaataatt tcttttttga
      901 ataaattttg tttaaaatat aacaagcagg gtataaagag actaaataac tattttagga
      961 ggagtacggt atgtcattga aatcactaaa aataaaagaa aatttatatt gggtaggttc
     1021 gcttgaccct gatttaagag tatttgacat aattatgtat acaccttatg gaactactta
     1081 taattcatat gtattaaaag gaacagaaaa aacagtttta tttgagacag ttaaagataa
     1141 gcattttgac aattatattg aaagattaaa tgatttaaat atagattttg aaaaaataga
     1201 ctatattgtt gtaagtcata ctgaaccaga ccatgcagga agcgttgaaa agcttttgga
     1261 tttagctaaa aatgcaaaag tagtagcttc agaaacagca attaaatatc ttaaggaaat
     1321 cgtaaacaaa gactttgaat atgttgcagt tacagatgga gatacattat caattgggga
     1381 caaaacttta gaatttttct ctgtacctat gcttcattgg ccagatacca tatacacata
     1441 tataaaagag gataaaactc ttgtaacatg tgattcattt ggtagtcact atagtaatga
     1501 taagatagta aatacacttg atgaaaatga agaaaaggat tacttggatg ctttaagata
     1561 ctattatgat tgtataatgg gtccatttaa accatctatg gtaactgcta tagaaaagat
     1621 aaaagattta gatatagata ctgtatgtcc aggtcatggt ccagtattaa ctgaaaatcc
     1681 tagaaaaatt atagaccttt attacaactg gagtgtaaat gaacaaataa agttagaaaa
     1741 agaagttaca atttgttatg tttctgcaca tggatataca aagattatgg cagaagctat
     1801 aaaagcatat attgaaaaaa atagcaacta taaagtaaat ttatttgatg taatagagca
     1861 taagcaagaa gatattttgg caaaaatagc agtatcacaa ggtgtattat ttggaacacc
     1921 aacaatatta ggagatgcgt taaagcctat atgggatata ttaatatcgt taaacccagt
     1981 tcttcatggt ggaaaagttg cttcagtatt tggttcatat ggatggagtg gagaaggtat
     2041 agaaaatgct atggagagaa taagtcagct tagaatgaca gcagtaaaac cttttgctgt
     2101 taactttaaa ccatcaaatg aagagattga taaattatat tcttatacag gtaaattcct
     2161 agataagtta aattctactt ttggaagtaa aaagaaaact aagaaattta aatgtgtaat
     2221 ttgtaatgaa gtttttgaag gtgatagtgc tcctagtgta tgccctgtat gtggagctaa
     2281 ggaagaccaa tttatagaag ttgaagaaga tgaagttact tttagaaaag atactgatga
     2341 atatttcgta atagtaggaa atggagcagc tggtttttat gcagcagatg ctataagaaa
     2401 aagaaataaa acttgtaaga taactatgat ttctaatgaa gatgagttga cttactatag
     2461 acctgcactt tctgatggta taaatgaaga acttggttca gatttctaca tggaagataa
     2521 agattggtat gataaaaata atatagtagt aatattagga acgaatgtag ataaattaga
     2581 tgaagtgaat aaaactatta tagtgaatga tggagctatt aaatttgata agcttgtaat
     2641 agctacagga agtagaaact ttataccacc aattaagggc catgatttag aaaatgtgtt
     2701 tactcttaga aatataaaag atttatatag tgtaaaagaa gcactagaaa aatctaagaa
     2761 agttgtagtg attggtggcg gtttattggg acttgaagct gcatgggaat ttagactaaa
     2821 aggattagaa gtagtagtag ttgaagccat ggatagcata ctatcaaaac aacttgataa
     2881 agaaggtagt aaaatattag aacaatgtgt aagagataca ggaatagatg taagattggg
     2941 tgtagcagtt gatggtattg aaggtgatgg aaaagctcaa aaagttgtat ttaaagatgg
     3001 agatagtgtc gactgtgata tggtagtatt ttcaattggt gtaagagcaa acactcaaat
     3061 ggttcaggat acatctgtaa aaattgatag aggtattgtt gtagataaga cattacagac
     3121 aaatgttaaa gatatatatg cttgtggaga tgttgcacaa gtgggaaata ttagtttagc
     3181 aatatggcca tcttcagtag aaatgggaaa aatagcaggg gcaaatgcaa gtggagataa
     3241 tttgacattt gaaagtgaag tatatccagt ttcattagat gcaatgaatg taaaagtatt
     3301 tagtattggt aatatacaaa attttgataa agaaatttca tcaaaagatg aaggtcaaag
     3361 aatatataaa aaattattta tgaaagatgg ttcattagtt ggagctatac ttataaatga
     3421 tttaagctgt actgttaaat tgataagatt aatatctgaa aaaggtgatt ttgaggatat
     3481 tatgaaggct gatatactat aatttaaata cattaaaata aaaaggagtt gtctcaaaat
     3541 tgattttttt ctaatcaaat tttaaattca actccttttt tgaattttta tataaaataa
     3601 catggaaatt ttagtaatgt ctgtggttac atacataggt ttaattgttt tttatatgga
     3661 ttgtctctgg tataataaat tgaattgtgt ttgactgata gatattttta ttgaaagtta
     3721 atacctgtta tgcgataata aggacgtgtt aggggagttt ttataataac aattaatctc
     3781 agtttttgtg ttccaatagt agcatcaaca cattctgatg tagtagttct tttcttataa
     3841 aaattagtta caaatatgct tgtaattatt tgatatgtaa atttaccaat attataaatg
     3901 atgacctcga taataaggag agtgaaaaat aatctatgaa cgaaaatatt ttgattgtgg
     3961 atgatgaaaa agaaatagca gatttaattg aagtatattt aaaaaatgaa ggttttacag
     4021 tatacaagtt ttatacaggt aaggaagctc tagaatgtat tgaatctaaa aatttggata
     4081 tggctatttt agatattatg cttcctgata ttgatggatt tcatatttgc cagaaaattc
     4141 gagaaaaata ctattatcct atcattatgt taacagctaa aattgaagat atagacaaga
     4201 taatgggttt aaccataggt gctgatgatt atataacaaa gccatttaac cctttggaag
     4261 ttgttgcaag agtaaagaca caattaagaa gatatatgcg atataataat tcttatgaac
     4321 aacagtcgat aattgttaat gagtatgata ttaaaggatt gattatcaat aaagaaacac
     4381 ataaatgtag tttatttggg aaagaagtag cattaacacc gattgaattt tctattcttt
     4441 ggtatttatg tgaacatcaa ggaaaagtgg ttccttctga agaattgttt gaagctgtat
     4501 ggggagaaaa atatcttgat aataataaca cagttatggc tcatataggg cgattacgtg
     4561 aaaaaattca tgaaccttct aaaaacccga aatttataaa aactgtatgg ggagtaggtt
     4621 atacaattga aaagtaaaaa tattaaagtc aatcctgact ttactcaagt gagaaataaa
     4681 atttttatga gaacagcttt aatggcattt atagccatta ttttcatatg gattttatat
     4741 tcttttatat tgtatggtaa gttttctatc tggatagtat cattctttga aaatgctttt
     4801 cgtatggaat ataaagctgc attgaattta tatcagcaga ttttccgtaa tagcatggag
     4861 ctcttcatga tactagctat ggtgattgtg ttttttataa tattccgggt ttatttaaat
     4921 tggtttacaa aatattttag tgaaataaat caaggtattg actctcttat caaagaagat
     4981 gttggagaag tggctttatc tccagagtta ttggcaatag aaaagaaaat aaactcaatt
     5041 aaacatatac ttgaacaacg taaatttgag acacaaatgg cagaacaacg taagaatgaa
     5101 cttattgttt atcttgcaca tgatttaaaa acaccactta catcagttat agggtatctg
     5161 acactacttc gagatgaaag tcagatttct gaagaactgc gaaaaaaata tttatctatt
     5221 tcgttggata aggcagagcg tcttgaagat ttaattaatg aattctttga aattacaagg
     5281 tttaatttat caaatatatc acttgaatac agtacagtaa gcttaactcg tatgcttgaa
     5341 caactaacat atgagtttaa acctactttg atgagtaaga atttaaaata tacactgaat
     5401 gttacaccaa atatgatgat aaggtgtgat gttaataaaa tgcaacgtgt atttgataac
     5461 ttattacgaa atgctgttta ttatagtttt gaagatacta ctattgaaat aacagctgta
     5521 caggaggatg attatataaa gcttaaattt ataaatcata gcaatacgat tccagaggaa
     5581 aaacttgagc gtatttttga acaattttac aggttggata catctcgtaa ttcaaacagt
     5641 ggtagagctg ggttgggact tgctatagct aaagaaatag tggagcttca taacgggaca
     5701 ataacagctc atagcgagaa caatataatt atctttaagg ttattattcc gattgtagga
     5761 aatttgtaag aatttaagta taaaaaaata ggaaattctt taggataaat aaataaagag
     5821 cactcttgtt attgatataa ttactgtatc aaaacaagag tgcttttatt ttgatacagt
     5881 aattatatca atagaaaaat gtgaaaatat attatgtgga acagatgcaa ttgtaagtga
     5941 aggttgattg gatttgaaat agaaaaagta ttttaacatg tttctgtaga ttgtataaaa
     6001 ataaataaat caacaaagga gaatcaaaga tgaataataa aaaaattgca gttatatttg
     6061 gtggaaactc aacagaatat gatgtatcat tacaatcagc ttttgcagta cttgagaatt
     6121 tgaatacgga aaaatatgat attataccaa ttggaattac aagggagggt aattggtatc
     6181 attatattgg agattataat aatatccaga ataacacttg gtttgaaagg gatactgata
     6241 taattccagt tattgtttcg cagaaccgtg tcaaaaatgg tattattgaa tttttaattc
     6301 ctgatgctgt ttctcagaat catattccaa atagtgctat tgaatcatgt gaaaatgata
     6361 ttaaatacac taaacttgat tttgtgtttc ctgtactgca tggaaaaaat ggagaagatg
     6421 gaacagtgca ggggttattt gaattggcag gtatacaggt tataggatgt ggtactcttt
     6481 cttcagcatt atgtatggac aaagacaaat cacataaact tgttcattca gaaggtatta
     6541 aagtaccaaa atcaatatta ctaaaatact cagataaatt taatgtggct gaactgtcaa
     6601 aagacttaac ttatccatta tttataaaac ctgtgcgtgc aggttcatca tttggtatta
     6661 gtaaaattta taaaaaagaa gagttacaag tagcagtaga cttggctttt gaacatgata
     6721 atgaagtaat aattgaggaa aatattgatg gttttgaggt agggtgtgca gttttaggaa
     6781 acgatgactt acttgttgga agagtagatg aaatagaatt gtcaaaagga ttttttgatt
     6841 atacagaaaa atatacattg aaaagttcaa aaattcatat gccagctaga attgatttgg
     6901 attgtgagaa aagaatacaa gagacagcaa aaattattta tagaactctt ggatgttctg
     6961 gatttgctag agtagatatg tttttaactc catctggtga aattattttt aatgaagtca
     7021 atacaattcc tggctttact tctcatagtc gttatccaaa tatgatgaaa ggtattggat
     7081 tgtcttttga agaaatattg gacaagataa ttgaggtgta cataaaatga tgaataatat
     7141 tttattagat aaaaataaag aagataaaat gacaaatact atcttattga ctaaagataa
     7201 agtttatgat gggaatctaa ttctagtaaa tgctttttta ccagttaaaa catctgaaga
     7261 tatagattta attcctgtag atacaaggtt tccaagtatt cttatgaaac gtgaagctac
     7321 aaacatttta caaaatattt taaaaagtat atgtggaata aatgaaatag tacctgtaag
     7381 tggatatcgt actgcagagg aacaacaaga tatatattca agttctctgc gtgacaatgg
     7441 aaaagacttt actaaaaagt ttgtagcact tccaaatcac agtgaacatc agacagggct
     7501 tgctattgat ttagggttaa agaaagaagt tatagatttt atctgccctg aatttcctta
     7561 taatggtatt tgtaatgagt ttcgtaaggc agcactacat tatggtttta tagagcgtta
     7621 tcagtgtaaa aaagaagaaa taacaggaat atctcaagaa ccatggcatt ttcgttatgt
     7681 gggatatccg cattctgaga ttatcaataa aatgaatctc gctcttgaag aatatataga
     7741 atatattaag agattttcgt atacaaattg tttaaaaata gaagataaag aaaaaattat
     7801 agaaatcttt tatgtgccta ttcatactaa agaagaagtg agaatctcta tacctgaaca
     7861 atgtgtatat caaatatcgg gtaacaataa ggatggtttt attgtgacat tttggagaaa
     7921 tagaaaggac taagaaatgg aggaacaaga atgattagga ataaatctta tgcagggctt
     7981 gattatttta ggattatagc tgcattactt ataattgcaa ttcatacatc tccattatta
     8041 tcatatagta aaatggctga ctttgtgtta actagtataa ttgctcgtat tgctgtgcca
     8101 ttctttttta tgctatcagg cttttttcta atttcaacat ataattataa ttgtaataaa
     8161 ctgaaatctt ttctaaaaaa tgtattattt atttacggga tagctattgt aatttatatt
     8221 cccttaaata tttacactgg atatttttcc atgaataatt tagtgcccaa tattattaag
     8281 gacattgtat ttgatgggac tttatatcat ttatggtatc tacctgcatc aattattggt
     8341 gcaagtattg catactttct tgtaaaaaga ctaggtttaa agtactcact agtaatcact
     8401 attgttattt atgtaattgg tatgtttggt gatagttatt atggtttttt agaaaaaata
     8461 ccactattaa agaatttata tgggtatatt tttgaagtat cagattatac acgaaatggt
     8521 atattttttg cacctgtatt tttcatactt ggtggaataa tatctaataa atctaattat
     8581 atgtcaacta aaaatagctt gattggattt cttatatcat tcttacttat gttatgtgag
     8641 ggaatgtttt tacataaact aggggtacag cgtcatgata gcatgtatat tatgttattg
     8701 ccatgtatgt atttcttatt tacagcctta acatttttga aaggttctcg agctaggtta
     8761 ttgcgaacat catcattaat tatttatatt atacatccta tgatgattgt tgttattcgt
     8821 atgttttcta aaatattcgg aatacaaact gtcttggttg acaatagcct tattcattat
     8881 atagtggtaa gtatagcatc ttttatagct tctataatta ttatattgat tcttaaacct
     8941 ctaaaaaaag acttgaaagg agaacataag gtaaatacca atagagcttg gattgaaatt
     9001 gatttaaata atttggaaca taatgtaaag atgatacaaa aagctatgcc agaagattgt
     9061 gaattgatgg cagttgtaaa agctaatgcc tatggtcata gtgcttttga agtagcgact
     9121 tgtgcaaacc aaataggtgt aaaagcattt gcagtagcaa caatagatga ggggattgat
     9181 ttacgtcgat atggaatact aggagaaata ttgattcttg gatatactaa cccaataagg
     9241 gcaaaggaac ttcataaata caatcttaca cagagtgtta ttgaccttaa tcatgctatc
     9301 tctttaaaca aacagaaata caacataaaa tcacacatta aaattgatac aggtatgcac
     9361 agactgggtt ttgatgttaa tgatgtgagg tcaattttaa aggcatttaa attaaaatac
     9421 ttagatatat gtggcatatt tacacacttg tgtgtttcag atagtctgaa agatgaagat
     9481 gtagacttta caaataaaca aattgaatgt ttttataagt taattgattt tctgttaaaa
     9541 aaaggtataa aaattcctaa agtacatatt caaagtagtt atggtttttt taattatccc
     9601 aatcttaagt gtaattatgt aagaattgga attgcattat acgggatatt aagttctcca
     9661 aatgatacga ctaaattgca acttgattta cgacctgttt tatccttaaa atcacaagta
     9721 attcttatta gaaaaataca aaaagatgat agttttggat atggaagagt ctttattgca
     9781 agcagagata gcgtaattgc tatattatct attggctatg ccgatggtat acctagaaat
     9841 ttatcctgtg gaaaaagtta cgtaataata aatggttgta gagcagcaat agtgggtcga
     9901 atttgcatgg accaacttgc tgttgatatt acagatatac ctaatgtaga agttggtaat
     9961 acggcaataa taataggaag agataattta tctgaattat ctgcttctga agttgcaaat
    10021 aactcatgta gtatatcgaa tgaattattg agcagagtag gaagaaggtt aaatgttata
    10081 aagaagtaac tcaattaaat agtaatattt gttgtactgt gtaatttatt ttactctgac
    10141 tagacatttt tagtaatatt atgtctaata tatatttttt ataacttaca tgacaattta
    10201 agttataaaa aggtgatgtt tcagattttt attttgaaac atcacctttt ttgttataat
    10261 atataataaa ataattatac cttaactgta aacttatata gattatttac tttttaatat
    10321 tagattcatt ttttgagatt ggtatataat aaacatatag agtatataat aatagttaaa
    10381 gttaaaaaag gggaaaagca atatgcagta tatacaagaa ctacttgatt tgactcaaaa
    10441 ttattgggtg ttagccacaa taatcggctt attaagtgca tttatagaaa gctttatacc
    10501 agccttacca cttgttgcta tagttacagc aaatgctgca atacaaggat tatttattgg
    10561 atgtttgtta tcatggattg gctcaggact tgggacaact tcattattct tattaataag
    10621 cagatttaat gatagtaagc tttttaataa gttgagaaat agtaaaactg aaaaagcaat
    10681 aagttggatg gataaacagg gatttaagtt attatttata gcgtatgcct gtccatttat
    10741 gcccggttgt ttagttacta tcgcatcagc ttttggtaaa aaagatttaa aagattttgt
    10801 gccagcaatg ttggcaggaa aatttgtaat gtttatagtt ataagttacg tggcaagtga
    10861 tatagaaggt tttataacaa gtcctttaaa gatagcatca tttatattat tggtgttttt
    10921 gtcatggaag attggaaata gagtaaataa aaatcttgaa aatcataatt atgatttcca
    10981 tcataaaaaa catcacaatg ataaagatga taaaatgatt tagcttgtac gaaataaaac
    11041 gtatcttaaa atgatttagt actaaattat tttaagatac gttttatttt ttgtattaag
    11101 gtacaaaatt tttagttctt ttgtaaacta aaatatataa aacttattta tacttagttc
    11161 aaaatattgt tatttatttt ttctacaact ataataaaat ataaaattga tatccaatca
    11221 aaaaaataac tatgaaattt aatagcttag atatgaattt ttctgttttt gagttagttt
    11281 ttataggtag taaagaaatg tttatggtaa tgggataaaa caattcaata ccttctttag
    11341 taataaggtc aagacacaag tgagatagat atcctattat aaacccactg gacaaacatg
    11401 agaaaactat attattgtta gtgaagattg tcagagattt aaaaaaacta taaataagta
    11461 ataaggcaat tagactgtga gtaaaccctc tatgtctaaa cctacttcca aataatttgt
    11521 atattaacat aaatttttta cttatgtaag aacctctcat atctatatct ggaaataaag
    11581 aacctaaata tgcaaaataa atataaatta taattaatac tattttatat gcaatatcgt
    11641 atttaactag gtatagattg tttataaagg ggagtatcaa aagtgcgaat atataacctc
    11701 ccctttcatg tgtttctttt atcatataaa aatcctcctg aatcgtcact ataaacatta
    11761 tattggtaaa tttagactat cttttattat attatatagc aaacataagt ttttttcaaa
    11821 aggattttaa tatttaatta atcttgagat tttaaatttt tgtaattttc taaagcacca
    11881 ttccaattta ctacattaaa ccagttgtca atgtattcat ttcttctatt ttgatatttt
    11941 aaatagtaag catgctccca aacatcaagt cctattatag gagttaggtt tttacttaca
    12001 gggctatcct gatttggagt agtcataata gataatttgc catctttagt agctacaagc
    12061 caagcccaac cagaaccaaa gacatctaaa gcagattttt ggaactcttg cttaaatttt
    12121 tcaaaagaac caaagtctct atcaatagct tcttttaaag attcagaagg tatggttttt
    12181 tctggcgtca ttatatcaaa aaagaattta tgattataag ctccacctgc attatttctt
    12241 acagttgtag caatatcttt aggtaaagaa tctaaatttt gcaataattc acataaagaa
    12301 taattataaa gctcaggata tttttcaaga gcagcattta atttatcaac ataagcttga
    12361 taatgcttat catgatgcag tttcattgtt tctttatcta tataaggttc aagtgcatca
    12421 tatgcataag gtaatggttt aaccttaaat ttgttatttt caggtgtaaa agcaaatgaa
    12481 gttatgcact gtgagattat aaataaagac ataataactg gtattaatat ttttttcttc
    12541 ataaaaatca cctcctaata tagtatttct agaaataaaa aaaatatgaa atattaacag
    12601 ataatttata agaatttaat agcaatttca ttttaaattg aataagtttt aaagccaaag
    12661 acaaaactat gaaatataga ataagactaa aggacagaaa ggaattttga ttatatggac
    12721 ggcaatattt caatttatta taaaggtgat atatctaaaa aagaagatat taaatttaaa
    12781 ataaaagaat ctgtaaataa tacaagactt tctagtagaa tagagtataa ggatgaggaa
    12841 aaaacttttg attttgaaag atttaatgta attgatgcta tttatggaaa taaaccattt
    12901 gaaaaagatg gaaaagttat tgtttttaat ggaaatatat ataactatca tgaaataaaa
    12961 gaagaattaa ttaaaaaagg acacaatttt actacaaatg atgatattga agtcttatta
    13021 gcatcttata tagaatttgg taagaattgt gtacataaga ttaaaggaat gttcaacttt
    13081 ataatctatg atagagaaaa tgaaagtata tttggagcaa gagatttatt tgggataaaa
    13141 ccactttatt atataaataa ggaaaatgca ataatttttt cctcagagta taagttttta
    13201 cttgaatata tgaaaaattt aaatataaat gaaagaagct tacaaagtta tttttcattt
    13261 caatatgtac ttcctgaaga tactatgata caaggaataa ggctgatacc agcaggtcac
    13321 tattttaggg tagaaaatgg tatactatct ttaaagagat ataataaact tgagtttaga
    13381 agtagtacaa aattttttta tactaaaaat cacttaggca atagagatgt gaatgaggat
    13441 gatataagaa atatagtagt agattcaata gtaactcata tggaagagga gaaagaaatt
    13501 ggaacatttt tgtctggtgg aatagattca tctattataa ctactgtggc ttcacaaatt
    13561 aatccaaata taaaatcttt ttctgcaggc tttagtgtca aaggatatag tgaattagaa
    13621 gtagcaaaga aaacagctga taagttaggt atagaaaata tccagattaa tataacacaa
    13681 gatgaatata taaaatcatt accgaatgta atttattctt tggatgaccc tatagcagac
    13741 ccttcagaag taggtcttta tttcttaatt aaagaggcag gtaaacatgt taaagtagca
    13801 ttgtctggag aaggagcaga tgaattattt ggaggatata atatatataa agagtatagt
    13861 actatgaaat cagttgtcaa ttcacctact tatattaaaa gtatattagg aagagtttca
    13921 gaacttatgc caaacataaa aggaagaaat tatctttata gagctacaac accattagaa
    13981 aaaagatata taggaaatgc aaaagttttt gaaaacagcg aagtaaaaag attctttttt
    14041 aagtataagg aaaagaatat atatgaatac ctgttgtcta atttgtatag agatgcacaa
    14101 aaaaataatt acgattatat aagtaaaatg caacatatag atgtgaatac atggcttcaa
    14161 ggcgatatac ttcaaaaaat tagcaaactt tctacagctg agcaggttga acttagagta
    14221 ccatttttat ataaagatgt atttgatgtc gcaaaaaatt taagaatgga gcaaaaaata
    14281 aataagaata atacaaaagt tttacttaga gaggcgttta gagaaatagt cccagaacat
    14341 gttgt
//
LOCUS       DAYAPA010000303        11835 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.guided.129, whole genome shotgun sequence.
ACCESSION   DAYAPA010000303 DAYAPA010000000
VERSION     DAYAPA010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11835
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.guided.129"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            158..1564
                     /locus_tag="ACGI59_003918"
     CDS             158..1564
                     /locus_tag="ACGI59_003918"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFG9741621.1"
                     /translation="MNTGFPKDFLWGASSSAFQVEGAWDKDNKGKTVADYNSFKKSHL
                     QADTKVASDFYHNYEEDIELMKELGMKTYRFSISWARIIPDGEGEINQKGLEFYNKII
                     DKLIECDIEPFVTLYHFDLPFKLVEKYNGWESRETVYAFERFAKICFKHFGDRVKYWQ
                     PHNEQNLIVRVEERINIYDETDSWKIDKMRAQMDYNLCLAHALAVNACHEMIKESKIG
                     AAVSSSVTYPLTSKPEDVYAARMNDNFKVYYMLDMHHYGEYPGYYMKYLEKRNIVPHM
                     EDGDKEILKKAKMDFIAVNYYRTNCAEALPEDSQHPFGLREGTVDFSMYGLFKMSMNP
                     NLEASEYGAAIDPSGLRVALNEYWQRYHLPVIITENGLGAKDILEDGKIHDDYRIDYL
                     RSHINACKLAIEDGVEMIGYCPWSFTDLLSSSQGFNKRYGLVYINRTDHEVLDLKRIK
                     KDSFYWYKEVIENNGIVK"
     gene            1592..2440
                     /locus_tag="ACGI59_003919"
     CDS             1592..2440
                     /locus_tag="ACGI59_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFG9741622.1"
                     /translation="MYITKVLNNSLLLAKDDNDEEIILMGKGIGHNYKAGSELNKKDI
                     EKIFVLHDENIKKSIIQLATEIDAEYFGIAQMIIAYGIEKYNLKLMNHIYLALTDHIA
                     FAVRRFKEGIMIENHYLFEIKEFNPKEYDIGKYAITVFKEVLGLDLPEEEIGNIAGHF
                     INAQQDNPYSDRNKRSAKIVNAILQIVHYHFSIVYNKESFYYRRFVMHLKAFSQRFLA
                     NEPSREKIDFIYEQVQKNCKEEYECVKKVGAYMYKEYSRELPRQEELYLMIHIHKILG
                     ELCENS"
     gene            3101..3793
                     /locus_tag="ACGI59_003920"
     CDS             3101..3793
                     /locus_tag="ACGI59_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFG9741623.1"
                     /translation="MNKVLIIDDDKDLCILLKKSISIEQIEANYCHSGRDGLVELSKQ
                     DYQLVVLDVMMPGMDGFETMEQIRKYSSIPILMLTSKSDNYSKVHGLRAGADDYLTKP
                     FEITEFTARVLSLIRRYTRFNPKNEEPQQLSFKGLLIDLDQHTVTTNGEPMLLPPKEF
                     GVLLYCARHQGKILTKQQIYEAVWGEPYVYDDSNIMAIISRLRKKIELDPSSPIYLQT
                     IKGIGYQFNTEV"
     gene            3790..4725
                     /locus_tag="ACGI59_003921"
     CDS             3790..4725
                     /locus_tag="ACGI59_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFG9741624.1"
                     /translation="MKMDEQTIILFLLLLSIGLLFLSIFAFSKLGHIYKRLKDIEEIL
                     ADVQIGDENRKILIKPCDVMAPLVYQLNEIVYDYENKLLSLKKSDKASKQLMTSLSHD
                     VRTPLTTLIGYLDAVHSGIVIEQEREEYLEIARRRAYDLKDYIDVLFDWFRLNSDEFT
                     LSIESVEIAELSRNILKDWIPIFHEKKLNFEIDIPENRLMVNLDPDGYSRVVNNLVQN
                     VLAHSKARQIKITMSEDSRMVLLRVEDDGVGIARENLPHIFERLYKCDKGRSEKGSGL
                     GLSIVSQMVERMGGQVSVESEIGKYTVFTVSLPLI"
     gene            4873..5796
                     /locus_tag="ACGI59_003922"
     CDS             4873..5796
                     /locus_tag="ACGI59_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019639192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9741625.1"
                     /translation="MNNLIIETKNLTKQYGAQKSVSNLNIHVQKGRIYGLLGRNGAGK
                     TTTMKMLLGLTKPTTGEIKIFGQDIHNNEKKLLPRIGCLIESPGFYPNLTGTENLKIF
                     ARLRGIPKKNAVESALDVVGLPNNDKKLFSQYSLGMKQRLAIALAIMHDPQLLILDEP
                     INGLDPIGIAEVRTFIRDLCTERGKTILISSHILSEIALLADDIGIIDHGILLEEESL
                     AQLEEKNSKYIHFAVSDAAQAARLLETQLNLKNFKVADNYNIMLYDTDVSVAEINRAF
                     ILNNIEVSESHLCEDTLEDYFKKVTGGEGIA"
     gene            5780..6550
                     /locus_tag="ACGI59_003923"
     CDS             5780..6550
                     /locus_tag="ACGI59_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9741626.1"
                     /translation="MKELLDIISCEFSKLKRLKFILISILGACLFPIPATILIAKDNL
                     PFEQLFKLVVNFGYFLLLPIVLSIVASQLFFIERDNDVLKNLATVPVQKGKLALAKLA
                     VLLFISLFYSVAGLGATIIGGFIVGIVEGVAVKLGMSIAFGIMFFVTVLPVVVLIVYF
                     NRSYIFSIILSFVYAIFNFSISLNIINFEPNNPILSVLPAPVIMRWWMAFWGDPTGEY
                     TALRQPYLLSTPACVGILCLITIIAVLLICMIYKKQEN"
     gene            6560..7342
                     /locus_tag="ACGI59_003924"
     CDS             6560..7342
                     /locus_tag="ACGI59_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFG9741627.1"
                     /translation="MLDIITTEFQKIKRYNILWIGVVAVLFSALLAVFQVVSSHGSDP
                     LTYESFANGVIWNNFSLSFPFGITLIGGYLINREYTDQTLKNILTVPISLRKLLVGKL
                     IAVGGITVLLSLFSFLCTFILGLLFCNINISVTLVVKSLVQILSVNLYCYVAVLPIIA
                     YFGRKQNAFLTGVGIAFVYGFCGVFVAGRNLTDFYPITAGLGLVGYNNGAGTVYQPLI
                     GAMTLIVILILTTILLVFTPNYDKVMTISKKKGIKHKKYSHK"
     gene            7719..8255
                     /gene="rbr"
                     /locus_tag="ACGI59_003925"
     CDS             7719..8255
                     /gene="rbr"
                     /locus_tag="ACGI59_003925"
                     /EC_number="1.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016692 - NADH peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin"
                     /protein_id="HFG9741628.1"
                     /translation="MNLKGTKTEKNLMAGFAGESEARNKYTYYASKARKEGYNQIAAI
                     FEETANNEKEHAKLWFKLLHDGMPSTEENLKDAAAGENYEWTDMYAKFAKEAREEGFD
                     KIAYLFEAVGKIEKEHEERYLKLLENLNEGKIFKRDEEVVWQCQNCGHVYVGTEAPEK
                     CPVCDHPKAYFNIKAENY"
     gene            8287..8676
                     /locus_tag="ACGI59_003926"
     CDS             8287..8676
                     /locus_tag="ACGI59_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HFG9741629.1"
                     /translation="MKFSKQRELILNEILNNPVHPTADYLYENLKKDNPNLSLGTVYR
                     NLAQLTEHGFIRKVSIPGYPDRFDGRIDNHYHIICEVCGEVYDLESEVLNNLQELISE
                     ETDIKITSYNISFKGICNNCKRCSQVG"
     gene            8713..9099
                     /locus_tag="ACGI59_003927"
     CDS             8713..9099
                     /locus_tag="ACGI59_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin family protein"
                     /protein_id="HFG9741630.1"
                     /translation="MCSEQKFFICETCGNLVGMIQSGGVPIFCCGKPMKELVPNTTDA
                     AVEKHVPVIEVDGNNVTVKVSSTTHPMTKEHHIAWVYLMTEQGGQRKCLAVDGEPVVK
                     FALNDDDKVISAYAYCNLHGLWKAEL"
     gene            9226..10668
                     /locus_tag="ACGI59_003928"
     CDS             9226..10668
                     /locus_tag="ACGI59_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HFG9741631.1"
                     /translation="MSIYKCSVCGYIYDESKNDKTWDELSEDWECPVCTKGRSYFGKI
                     STVYYEEDEKIAEDIVEDESKLNTEKEGDLNYLSTYLRRDDEVEKHMDIIHEMAVTGK
                     SIIEPMRTKLPVISWDDILIMGAQLNPLPLNEHDEVNTTTIIGKKAKKPMIIENPVYI
                     SHMSFGALSKELKIALAKGAAQNKTAMCSGEGGILPEEKEASYKYIFEYVPNKYSVTE
                     ENLKNSDAIEIKIGQGTKPGMGGHLPGEKVTEEIAKVRNKPVGQDVISPSCFEEIQSK
                     EDLKKLVDELREVSEGRPIGVKISAGHIEKDMEFIAYAKPDFVTIDGRGGATGASPKL
                     LKDATSIPTIFALYRARKYIDTHGLDIDLVITGGLRISTDFAKAIAMGADAVAIASSA
                     LMAAACQQYRICGSGKCPVGVATQDEELRKRLHIENSANRVANFLNVSLEELKTFARI
                     SGHKDIHDLSVDDLYTVNSEISNYTNIQHV"
     gene            10965..11780
                     /locus_tag="ACGI59_003929"
     CDS             10965..11780
                     /locus_tag="ACGI59_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFG9741632.1"
                     /translation="MERLKIQVLVDNNTYIDRYFVGEPAVSYYIEIDGNRILFDSGYS
                     DVFISNAEKLNIDLGNLTHVVFSHGHNDHTRGIQFLENRYDLSTVELISHPNCFIPRK
                     HGEKSIGAPFSAEEIKNIFMYNPKDKPFNLSKNCVFLGEIPSINDFEKRAKIGKCKIG
                     DLWEDDYVLDDSAIVCKTDKGIFIVTGCSHSGICNIVEYAKKVCGDDRVIGILGGFHL
                     FELNNRLDSTIQYLDKEEMRMLYPCHCVSFKVKAKMNEILNINEVGVGLTISI"
BASE COUNT         4250 a         1478 c         2258 g         3849 t
ORIGIN      
        1 aatatagagt aagtttattg taatgaattg ttaaagaaaa taattaatat agggtaaatc
       61 tatctcaatg aaataacaaa gaaaagagtt aatatagagt gaacttattg caatgaatca
      121 acaaagaaaa taatcaatat aaaaaggaga aatatttatg aatacaggat ttccaaaaga
      181 ctttttatgg ggagcatcat cttctgcatt tcaagtggaa ggagcttggg ataaagacaa
      241 taaaggaaaa accgtagccg actataattc ttttaaaaag tctcatttac aggcagatac
      301 taaagtcgca tcagactttt accataatta tgaagaagat attgaattaa tgaaagaatt
      361 agggatgaaa acatatcgtt tttcaatttc atgggcaaga ataataccag atggtgaagg
      421 agagataaac cagaaaggtt tagaatttta taataagata atagacaaac tgatagagtg
      481 tgatattgag ccattcgtaa ctttatacca ttttgacctt ccatttaaat tagtagagaa
      541 atataatggt tgggaaagta gagaaactgt ctatgctttt gaaagatttg ctaaaatatg
      601 ctttaagcat tttggagata gggtaaagta ttggcaacct cataatgaac agaatttaat
      661 tgttagagtg gaggagagaa ttaatatata tgatgaaaca gattcatgga agatagataa
      721 aatgcgtgct cagatggatt ataatctttg cttagcacat gcattagcag tcaatgcatg
      781 tcatgagatg ataaaagaaa gtaaaattgg agcagcagta tcatcatctg taacatatcc
      841 tcttacttct aagccagaag atgtatatgc agctagaatg aatgataatt ttaaagtgta
      901 ctatatgctt gatatgcatc attatggaga atatccaggg tattatatga agtatcttga
      961 aaaaagaaat atagttcctc acatggaaga tggggataaa gagatactta aaaaagctaa
     1021 aatggatttt atagctgtaa attattatag gacaaattgt gcagaagctt tacctgaaga
     1081 ttcacaacat ccatttggat taagagaagg aactgttgat tttagtatgt atggactatt
     1141 taaaatgtca atgaatccta acttagaggc ttctgagtat ggagcagcaa tagacccatc
     1201 aggtcttaga gttgccttaa atgaatattg gcaaagatat cacttacctg ttataataac
     1261 agaaaatggt ctaggagcta aagatatatt agaagatgga aaaattcatg atgactatcg
     1321 tatagactat ttaagaagtc atataaatgc atgtaaacta gctattgaag atggtgtaga
     1381 gatgattgga tattgtccat ggtcatttac agacctttta agttcttctc aaggttttaa
     1441 taagcgttat ggacttgttt atataaatag aactgaccat gaagttttag atttaaaaag
     1501 aatcaagaaa gatagttttt actggtataa agaagttata gagaacaatg gaatagtaaa
     1561 ataaacataa atatatattg agggttttag tatgtacatt acaaaggtat taaataattc
     1621 gttgctactt gcaaaggatg ataatgatga agaaataata ttgatgggga aaggcattgg
     1681 ccataattat aaggccgggt ctgagttaaa taaaaaagat atagaaaaga tttttgttct
     1741 tcatgatgaa aatataaaaa agagtattat acaattggca acagaaatag atgcagaata
     1801 ttttggtata gctcaaatga taattgcata tggaatagaa aaatataact taaaacttat
     1861 gaaccatata tatttggcct taactgacca cattgccttt gcagtgagac gttttaaaga
     1921 aggtataatg attgaaaatc actatttgtt cgaaatcaag gaatttaatc caaaagagta
     1981 tgatatagga aaatatgcta ttactgtttt taaggaggtt cttggtttag acctaccaga
     2041 agaagagatt ggtaatatag caggccattt tataaatgca caacaagata atccttatag
     2101 tgatagaaat aagagaagtg ctaaaattgt aaatgctatt ttgcaaatag ttcattatca
     2161 cttttctatt gtatataata aagagtcatt ttattatagg agatttgtaa tgcatctaaa
     2221 ggccttttca caaagatttc tggctaatga accatcaaga gaaaaaatag attttatata
     2281 tgagcaagtt caaaaaaatt gtaaggaaga gtatgaatgt gtgaaaaagg taggagctta
     2341 tatgtataag gaatactcca gagaattacc aagacaagag gagttatatt tgatgattca
     2401 tattcataag attttaggag aattatgcga aaatagttag aatatcttaa tatgctagta
     2461 atattataaa aaattaaaaa ttaactgcac tttaaatatg aataattgca ttacttaagg
     2521 gggttgtctt aaaattgatt tttttaatta aatttgaagc tcaactcctt ttttaaacct
     2581 ttatatcatt acaactttaa tgtttatacg actgtacttt agatgtatat ctacttttta
     2641 catttctcaa catgcaaacc taccattttt tgcagtatgt taattgaaat aaaccattga
     2701 gttaacccta aatttattct ggacttatag gaatagttcc aaagatactt taaaatattt
     2761 tctggaataa ttgcttattt cacatatgtt ttattgtgat gttttgattt aataaattct
     2821 tcaactctgt atatagtaaa tgtattatca tgtttgtttt attcaagcac acttataata
     2881 cgcttgattt tttcacaatg atgaaatata ctaatgggtg ttataggtat aagaatcgca
     2941 ttacacttga agaagtaagt gataaatgag gtataaatcc tcatatgata aactactatt
     3001 tcattgagga acatattcta agtgatgtca agatactaca atatgactaa attcctagtg
     3061 ataaaaaagt ctacgaatga gtgtagaaag gggaactaag atgaataaag ttttaattat
     3121 agatgatgat aaagatcttt gtattttgtt aaaaaagagt atttcaattg aacagattga
     3181 agcaaactat tgccattctg ggagagatgg actggttgaa ctttcaaaac aagattatca
     3241 gttagtagtt ctagatgtga tgatgccagg tatggatggt tttgaaacta tggaacaaat
     3301 acgaaaatat agcagtattc ccatcttaat gctgacatca aaaagcgata attacagcaa
     3361 agtgcatgga ttgcgagctg gtgcagatga ctatctaacc aaaccatttg aaattacaga
     3421 atttactgct cgtgtactgt ctttaatccg acgttacacc cgttttaatc caaaaaatga
     3481 ggagccacag caactttctt tcaaaggttt gttgattgac cttgaccagc atacagttac
     3541 taccaatggc gaacctatgt tgcttccacc caaggaattt ggtgtacttc tttactgtgc
     3601 aaggcatcag ggtaagattt tgacaaaaca gcagatttat gaagccgtgt ggggtgagcc
     3661 atatgtatat gatgacagca atatcatggc tattatcagt cgcctaagaa aaaaaattga
     3721 actagaccca tcatctccca tctatttgca aacaataaaa ggaattgggt atcaattcaa
     3781 cacggaggta tgaaaatgga tgaacaaacg ataattcttt tcttactctt gctgtcgatt
     3841 ggtctacttt ttctttccat atttgctttt tccaagcttg ggcatattta caagcgccta
     3901 aaagatatag aggagatact ggctgatgta caaattggag acgaaaatcg taaaatacta
     3961 attaagccat gtgatgttat ggctccattg gtttatcagc ttaatgagat tgtctacgat
     4021 tatgaaaata agttactttc tctgaaaaag tcggataaag ctagcaaaca acttatgaca
     4081 agtttatctc atgatgttcg tacaccatta acaactctga ttggttatct ggatgctgta
     4141 cacagtggta tagttatcga acaagagcgg gaggaatacc ttgaaatcgc taggcgtaga
     4201 gcctacgacc tcaaggatta cattgatgtt ctttttgact ggtttcggtt gaactctgat
     4261 gaatttacgc tgtcaataga atctgtggaa attgcagagt tgtcacgcaa tattttgaaa
     4321 gactggattc ctatattcca tgaaaagaaa ctgaattttg aaattgatat tccagaaaat
     4381 cgactaatgg taaacctcga cccagatgga tattcccgtg tggtcaataa tctggttcaa
     4441 aatgttcttg ctcacagtaa ggctcggcaa atcaagatta ccatgagtga ggattccaga
     4501 atggtattac tccgtgttga ggatgatggt gttgggattg ccagagaaaa tttaccacat
     4561 atttttgagc gactgtataa gtgtgataaa ggacgttcag aaaaaggaag tggtcttggt
     4621 ctatctattg tcagtcaaat ggtggagcga atgggtggtc aggtttccgt agaaagtgag
     4681 attggaaagt ataccgtatt tacagtgagc ttaccattaa tataaaattg tttttccccc
     4741 atgtgacatg tatacacatg gggatttttt gtaagttata aagttgcaag gttaatacaa
     4801 ggttgttgca aggttaatgt aagattgagg tggtatgata actttaataa acaaaggagg
     4861 cttttattat ttatgaataa tttaattatt gaaacaaaaa atctcactaa gcaatatggt
     4921 gctcaaaaga gcgtttctaa tttgaatatt cacgttcaga aaggtcgcat ttacggattg
     4981 ctaggaagaa acggtgcggg taagacgaca actatgaaaa tgttattggg tctaacaaaa
     5041 ccaactactg gagaaattaa aatatttggt caggacattc acaataacga gaagaagctt
     5101 cttcctcgaa ttggatgttt gattgaatca ccagggtttt atcctaatct gacaggaaca
     5161 gaaaatctca aaatctttgc aaggcttaga ggaataccaa aaaaaaatgc agtagaatca
     5221 gcactggatg tagtcggttt gccaaacaat gacaaaaagt tattttctca atactctctt
     5281 ggtatgaaac agaggttggc gattgctctt gccattatgc atgacccaca actgttgatt
     5341 ttggacgagc ctatcaatgg acttgatcct attggtattg cagaagtaag aacgtttatt
     5401 cgtgatctct gcacagaacg aggtaaaact attttgattt ccagtcacat tctttctgaa
     5461 attgccttgt tggcagatga cattggcatt attgaccatg gtattttgtt ggaggaagaa
     5521 agtcttgccc aattagaaga aaaaaacagt aaatacatac attttgctgt atctgatgca
     5581 gcacaggcag caaggcttct ggaaacccaa cttaacctga aaaatttcaa agtagcagat
     5641 aattacaata ttatgctata tgatacagat gtttctgttg cagaaataaa tcgtgctttc
     5701 attctaaaca acattgaggt atcagaatct catttatgcg aagatactct tgaagattac
     5761 ttcaagaaag taacaggggg tgaaggaatt gcttgatata atttcatgtg aattttcaaa
     5821 actcaagcgg ttaaaattta tactgatttc tattttaggt gcttgcttat ttccaattcc
     5881 cgcaacaatt ttgattgcaa aggacaactt gccctttgag caattgttca aattagttgt
     5941 caattttgga tattttttgc tattaccaat tgtattaagt attgttgcta gccaactatt
     6001 ttttatagaa agagataatg atgtattaaa aaatcttgca acagtgccag tgcaaaaagg
     6061 aaaactggct ctggcaaaat tggctgtgtt gctgtttata tcattgttct actctgttgc
     6121 agggttagga gctactatca ttggaggttt cattgttgga atagtagagg gggttgctgt
     6181 aaaattagga atgagcattg cttttggaat tatgtttttt gttactgtac ttcctgttgt
     6241 ggtactcatt gtatatttca atcgcagcta tattttttct attatacttt cttttgtata
     6301 tgctattttc aattttagta tatccttaaa tattatcaat tttgaaccaa ataacccaat
     6361 attaagtgta ttacctgctc ctgttataat gcgttggtgg atggcatttt ggggagatcc
     6421 cactggcgaa tacacggcac ttagacagcc atatttgctc tcaactcctg cttgtgtagg
     6481 tattttatgt ttgataacca ttatagcagt cctgctgatt tgtatgatat acaaaaagca
     6541 ggagaattag gaggtatcta tgcttgatat aattactaca gaatttcaaa aaataaaaag
     6601 atataatatt ctatggattg gtgttgttgc tgttctgttt tcagcactat tggcagtttt
     6661 tcaggttgta agttcacacg gcagtgaccc tctaacgtat gagagctttg caaacggagt
     6721 tatctggaat aatttcagtc ttagttttcc atttgggatt acacttattg gaggatatct
     6781 tattaaccgt gaatatacag accaaacctt aaaaaacatc ctaactgttc ctatttcatt
     6841 acgcaaattg ctagtgggaa aattaattgc ggttggagga attactgtat tgttaagtct
     6901 tttcagcttc ttatgcacat ttattttggg gttgttgttt tgcaatatca atatatctgt
     6961 tacactcgtt gtaaaatcct tagttcagat tttgtctgtt aatctgtatt gttatgttgc
     7021 tgttttgccg attattgctt attttggtcg taaacaaaat gcatttctga cgggtgtagg
     7081 gattgcattt gtatacggtt tttgtggtgt gtttgttgct ggaagaaatc tgacagactt
     7141 ttatccaatc acagcaggtc ttggtctagt tggatacaac aatggtgctg gtactgttta
     7201 tcagccactt atcggtgcta tgactttgat tgtaattcta atcttgacaa ctattctact
     7261 tgtatttaca cctaattatg ataaagttat gaccatatct aagaaaaaag ggataaagca
     7321 taaaaagtat tctcataaat aacataaata ttgtttctat tatttaagtt gctagacagt
     7381 gaaaagaaaa aatctgtcgt acagtgtcta tatgctcatg tattcaagaa gctacagttt
     7441 tgatttttca taactgtagc ttctttttgt atttattaaa tcacgactat cataatactt
     7501 gcaataggta tagcgacaag agagagtact gtcatatgtg ttttatatat tacattaagt
     7561 atattctaaa atatatgttt taaataatat atttgaaaat aggaatattt aaataaaaaa
     7621 aataatatta tatatagttg acaaaaattg gcaaatgaga taatatataa atatagaaat
     7681 agtaatcatt actattatta aaaagggagg aattaattat gaacttaaaa ggaactaaaa
     7741 cagaaaagaa tttaatggca ggatttgcag gagagtcaga agcaagaaat aaatatacat
     7801 actacgcttc aaaagcaaga aaagaaggtt ataatcaaat agctgctata tttgaagaaa
     7861 ctgctaacaa tgaaaaggaa catgctaaat tatggtttaa acttttacat gatggtatgc
     7921 cttcaacaga ggaaaattta aaggatgcag cagcaggtga aaactatgaa tggactgata
     7981 tgtatgcaaa atttgctaaa gaagctagag aagaaggatt tgacaagata gcttatttat
     8041 ttgaagctgt tggaaaaata gagaaagagc atgaagaaag atacttaaag cttttagaaa
     8101 acttaaatga aggaaaaata tttaaaagag atgaagaagt tgtttggcaa tgtcaaaact
     8161 gtggtcatgt gtatgtaggt actgaagcac cagaaaaatg cccagtatgt gaccatccaa
     8221 aagcgtattt caatataaaa gctgaaaatt attaaaaatt atcattatta aaaaggaatg
     8281 ttaactatga aattttctaa acaacgagaa ctgattttaa atgaaatatt aaataatccg
     8341 gttcatccta ctgcggatta cctatatgaa aacttaaaaa aagataatcc aaatttaagc
     8401 ttaggaactg tgtatagaaa tttagctcag ctaacggaac atggctttat aagaaaggtt
     8461 agtattccag ggtatccaga tagatttgat ggtcgaatag ataatcacta tcatatcata
     8521 tgtgaagtat gtggagaggt atacgattta gaatccgaag ttcttaataa tttacaagaa
     8581 ttaatatctg aggagacaga cataaaaata acatcttata acataagttt taaagggatt
     8641 tgtaataatt gtaaaaggtg tagccaagta ggttagtttt caaattctaa aaataattaa
     8701 tgaggtgagt ttatgtgtag tgaacaaaaa ttttttatat gtgagacttg tggaaacctt
     8761 gtaggaatga tacaaagtgg tggtgtacct attttctgtt gtggaaaacc tatgaaggaa
     8821 cttgttccta atacaacaga tgcagcagtt gaaaaacatg tacctgtaat tgaagtggat
     8881 ggaaataatg ttactgttaa agtaagtagc acaactcatc caatgactaa agaacatcat
     8941 atagcatggg tgtatcttat gactgaacaa ggtggtcaac gcaaatgtct agcagtagat
     9001 ggagaacctg tagttaaatt tgctttgaat gatgatgata aagtgatttc tgcctatgct
     9061 tactgtaatt tacatggatt atggaaggct gaactgtaat atttaaataa attgagtcaa
     9121 aaaggaggct ataaatgtat tttcatacaa atagcctcct ataaatttat ataaattcaa
     9181 gttttaattt tgtttaatgt caatataata taaggagaaa ataatatgtc aatttataaa
     9241 tgtagtgttt gtggatatat ttatgatgaa tctaagaatg ataaaacatg ggatgaatta
     9301 agtgaagatt gggaatgccc tgtatgtaca aaaggtcgta gttattttgg aaagataagt
     9361 actgtttact atgaagaaga tgaaaagata gcagaagaca tagttgaaga tgaatctaaa
     9421 ttaaatactg aaaaagaggg agatttaaat tacttaagta catatttaag aagagatgat
     9481 gaagttgaga aacatatgga tataattcac gaaatggctg ttacaggaaa atctataata
     9541 gaacctatga gaactaaact accagtaatt tcttgggatg atattttaat tatgggtgct
     9601 caattaaatc cattaccttt aaatgagcat gatgaagtaa atacaacaac aataatagga
     9661 aaaaaagcaa agaaacctat gattatagaa aatccagttt atatttccca catgtcattt
     9721 ggagctttgt ctaaagaact aaaaatagct ttagcaaagg gtgcagccca aaataagaca
     9781 gctatgtgta gtggtgaagg aggtatatta ccagaagaga aggaagcttc atacaagtat
     9841 atttttgagt atgtaccaaa taaatatagt gtaactgaag aaaatcttaa aaattctgat
     9901 gctatagaga taaaaattgg tcaaggaaca aaacctggta tgggaggtca tcttcctggc
     9961 gaaaaagtaa ctgaagaaat agcaaaagta agaaataagc ctgtaggtca agatgtaata
    10021 agtccatctt gttttgagga aatacaatcc aaagaagatt taaagaaact tgtagatgaa
    10081 cttagagaag tttcagaagg gcgtccaata ggtgttaaaa tttcagcagg tcatattgaa
    10141 aaagatatgg aatttatagc ttatgctaaa cctgattttg taactattga tggtcgtggt
    10201 ggagctactg gagcaagtcc aaagttgctt aaagatgcaa cttcaattcc aacaatattt
    10261 gctctttatc gtgctagaaa atacatagat acacatggat tagatataga tttggttata
    10321 acaggtgggc ttcgtatatc tactgatttt gctaaggcaa ttgctatggg tgctgatgca
    10381 gttgctattg caagttctgc tcttatggca gcggcgtgtc aacaatatcg tatatgtggt
    10441 tctggtaagt gtcctgtagg agtagctact caggatgagg aattgagaaa aagattacat
    10501 atagaaaaca gtgctaatag agtggcaaac tttttaaatg taagtttaga agaacttaag
    10561 acttttgcta gaatttctgg tcataaagat atacatgatt taagtgttga tgatttatat
    10621 actgttaact ctgaaatatc aaattataca aacatacagc atgtatagat tgaagtttga
    10681 aactattaga ttttagatag ctataaaagt aaatgataaa tatattttat aaaagagagt
    10741 gtcttaaaat gaacttttta gttcataagt cactcttttt tttatgaagc caaatatatt
    10801 tatcatttta gcaatgaaaa agaatcttat ctctttattt tgagatagac tccttctatt
    10861 tatgaaaatt aaagatatta aattgtaaaa tattctgtga tgtcgtacta ttaaactaaa
    10921 gattctgtac tttaaagtaa ataaaaattt aaggagtgat tattatggaa agattgaaga
    10981 tacaagtttt agttgacaat aatacttata ttgatagata ctttgttgga gaaccagcag
    11041 taagctatta tattgagatt gatggaaata ggattttgtt tgactcaggg tattcagatg
    11101 tattcatatc aaacgctgaa aaattaaata tagatttagg aaatttaact catgttgttt
    11161 tttctcatgg gcataatgac catacaagag gtatccagtt tctagaaaat aggtatgatt
    11221 tatctacagt tgaacttatt tcacatccta actgttttat acctagaaaa catggagaga
    11281 aaagcatagg agctccattt tctgctgagg aaattaaaaa tatttttatg tataatccta
    11341 aggataagcc atttaatcta agtaagaatt gtgtgttttt aggtgaaata ccttctataa
    11401 atgattttga aaaaagagct aaaattggta aatgtaaaat aggagatttg tgggaagatg
    11461 attatgtttt agatgattct gctatagttt gtaaaactga taaaggtatt tttattgtta
    11521 caggatgttc tcatagtgga atttgtaata tagtagagta tgctaaaaag gtatgtggag
    11581 atgatagagt tattggtata ctaggtggtt ttcatctttt tgaattgaat aataggttag
    11641 atagtacaat acaatatttg gataaagaag aaatgagaat gttgtaccct tgtcactgtg
    11701 tttcttttaa ggtaaaagcg aagatgaatg aaatattgaa tataaatgaa gttggagtag
    11761 ggctgaccat aagtatatag tatttgaaag tatataagat ttaagttgaa ggttaaaaag
    11821 atattttcta tagaa
//
LOCUS       DAYAPA010000304        11041 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.guided.137, whole genome shotgun sequence.
ACCESSION   DAYAPA010000304 DAYAPA010000000
VERSION     DAYAPA010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11041
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.guided.137"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            243..581
                     /locus_tag="ACGI59_003930"
     CDS             243..581
                     /locus_tag="ACGI59_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFG9741633.1"
                     /translation="MANIGKIIGDRIKVYRTKLGYSQEFLAEKAGLHPTYIGQIERAE
                     TNTSINIIMKIATALDIPLELLFANIITTEGVDNSVPLECYEMINKLTEKEQIAMLEL
                     IKCIIKYKEI"
     gene            603..920
                     /locus_tag="ACGI59_003931"
     CDS             603..920
                     /locus_tag="ACGI59_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFG9741634.1"
                     /translation="MNYIGKRLKEERKKANLTSKEFANMVGVSPWYITQIESGKKNPS
                     LKTFIKFVNILNISADVLIKDITSTGKTYLENDINEELKDLNSRELNLISQIIDIIKK
                     NKI"
     gene            1070..1747
                     /locus_tag="ACGI59_003932"
     CDS             1070..1747
                     /locus_tag="ACGI59_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9741635.1"
                     /translation="MIVIEGSDKFKIAKEYIDVEYTLFSKVTFRYEKLKFKDNAELEK
                     IKMFKYKNGYIPNKLNLSFGYGFSSYKKQIIRETVDTLRLTEIFSSENIEDIKFIKDG
                     TKKLEISIEKVVKFKRRKKRNYVCCYCPDMYRDIKLDKESINKIYNRKIKIEREVNIF
                     EDEDVIINKKVLKFPKSWTKNMQKYWLSENKYPIHSTVIDDDRYKCCNVQYTKNRVII
                     LYYIYNH"
     gene            complement(2075..2842)
                     /gene="aroD"
                     /locus_tag="ACGI59_003933"
     CDS             complement(2075..2842)
                     /gene="aroD"
                     /locus_tag="ACGI59_003933"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFG9741636.1"
                     /translation="MKRKVQVKNITIGEGRPKICVPIIGKNKKDIIKEAKELKDACLD
                     IIEWRVDFFENVENIKEVKEVLYELRSYIHDIPLLFTFRSVVEGGEKLISRDYYTTLN
                     KEISNTGLVDLIDVELFMGDEVIDEVVNFAHKKEVKVIISNHDFNKTPKKEEIVSRLC
                     RMQELGADLPKIAVMPQNEKDVLVLLEATNEMFKIYADRPIITMSMSGMGVISRLCGE
                     IFGSALTFGAAKSVSAPGQISFKELNSVLNLLHKSIN"
     gene            complement(3514..4350)
                     /locus_tag="ACGI59_003934"
                     /pseudo
     CDS             complement(3514..4350)
                     /locus_tag="ACGI59_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020220015.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            4421..5089
                     /locus_tag="ACGI59_003935"
                     /pseudo
     CDS             4421..5089
                     /locus_tag="ACGI59_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            5216..5836
                     /locus_tag="ACGI59_003936"
     CDS             5216..5836
                     /locus_tag="ACGI59_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFG9741637.1"
                     /translation="MHLIDFMNEDIKKKLVKKTYKKKETILFAEQENEYVILMIDGIA
                     EAFILNVKGNISTIHLYRSGSFFGEMEQFNDGKKPVEIVAFTDCNVYKLHRDDFLNWL
                     RGDFEATKFLIREISSKLVVNAELIEELSSLSVKERLLRCISLHYYRSELNLLTKKQL
                     ASEVNAPIRSINRAINECVQEHLIAYENKRFSVINQREVLKNLPTT"
     gene            5992..6774
                     /locus_tag="ACGI59_003937"
     CDS             5992..6774
                     /locus_tag="ACGI59_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
                     /protein_id="HFG9741638.1"
                     /translation="MTKTQQKMIQNVLEFSKRDSRIIGLAISSSYITKSLDEYSDLDF
                     LIVADDKSYEEVIEERLLIVEQFGTLVSAFTGEHVGEPRLIISLYDSPVLHVDFKFTT
                     LDGLLDRVEDSAILYEENNCITEIYSKKEAYFPTPDLQWIEDRFWIWVHYACTKIGRR
                     ELFETIDFLSFLRQSVLAPLIQLLLGKLPRGVRKIELDAPQYIEALEKTVANHEYKSC
                     VNALQATINLYLELREELSQSPIVRKVEAERVALQYFQDLIK"
     gene            6899..7825
                     /locus_tag="ACGI59_003938"
     CDS             6899..7825
                     /locus_tag="ACGI59_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFG9741639.1"
                     /translation="MSDILSRITNKIGDKNIVDKLSALSKSDLNSLLLEVFDRQANTL
                     TATDILKSYQLNRFTIPSGIDPEEIHALESKLLRKAFNMDIKTIMLSPSAPLGSCSVF
                     GSVDQYNVVSALRGTETLADPSNMLAIIIADKLKRKEENNTIPIHMATTARVIRAQNF
                     VGKGLYSHFGLYCMVSSGIDTGSYTCEKMLLEKHLNYYKDILSEIENVRFSIILRKRN
                     GYKDNDGFFDRMVETIKLIFPNIELSIHNDDVDNNYYKGINFKLSVKQGNETVEIVDG
                     GFVDWTYQMLGNKRERCLISGIGLERFLVAVS"
     gene            7839..8288
                     /locus_tag="ACGI59_003939"
                     /pseudo
     CDS             7839..8288
                     /locus_tag="ACGI59_003939"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            8648..9055
                     /locus_tag="ACGI59_003940"
     CDS             8648..9055
                     /locus_tag="ACGI59_003940"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFG9741640.1"
                     /translation="MVDEKTIEHQFDSFCKTVLRNYARDIYDENKRRNDYLVSLESLS
                     LAELSKLSILDDYDSNYICMVSYDYNIRIEDVLMAQAIGKLTKRKQDIILLSFFFNMT
                     NADIATLMDLAENTIHYHKTNALKELKELMQEH"
     gene            9057..9287
                     /locus_tag="ACGI59_003941"
     CDS             9057..9287
                     /locus_tag="ACGI59_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFG9741641.1"
                     /translation="MKPTKEVLPFTIILLAVHGDVNAINVILKHFEHYIIRLSQKTLF
                     DEYGNPYIHVEEETKRMLGTKLITAILRFNLS"
     gene            9677..9880
                     /locus_tag="ACGI59_003942"
     CDS             9677..9880
                     /locus_tag="ACGI59_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFG9741642.1"
                     /translation="MNNTDVPIWEKYTLTIEEASKYFRIGEKKLRKRADENLDSGWVI
                     MNSNRIQIKRKQFEKIVDTLDVI"
     gene            9958..10290
                     /locus_tag="ACGI59_003943"
                     /pseudo
     CDS             9958..10290
                     /locus_tag="ACGI59_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009271880.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase DNA-binding domain-containing protein"
     gene            10383..11015
                     /locus_tag="ACGI59_003944"
     CDS             10383..11015
                     /locus_tag="ACGI59_003944"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HFG9741643.1"
                     /translation="MTIPSSNKVYPRSNDYQTVYLKNVITRENIEVGDYTIYNDFYND
                     PRDFEKNNVLYHYPINNDKLIIGKFCSIACNAKFLMTSGNHSMKSKSTYTFPIFYEEW
                     KLDINHITDAWDNKGNIIIGNDVWIGYDSIIMSGVNIGDGAIIGTRAVVTNDVPPYSI
                     VGGVPAKIIKKRFNNDTISKLLEIKWWDWPYKKIQSNIKHIQSGNIDKLV"
BASE COUNT         4065 a         1567 c         1742 g         3667 t
ORIGIN      
        1 taattaacat attattaatt cgttttatta ttgtagtatg gctatgaaat tatgaaaata
       61 tttctctaat taaaaatatg atttatccat atttgtttat tgtagataca gactatacaa
      121 aaataaatag cacttatttc agtataaaaa catatatagt ctatatttag actaaaaaag
      181 tataaatagt ctatataaaa caacttaaac tatgaatata atctaaagtg gagggataat
      241 aattggcaaa tataggaaaa ataataggag atagaataaa agtctataga acaaaattgg
      301 gatatagtca agagtttcta gcagaaaagg caggtttaca tccaacatat attggtcaga
      361 ttgaaagagc tgagacaaat acatctatca atattataat gaaaattgct actgctttag
      421 acataccact tgaattgtta tttgcaaata ttattacaac agaaggcgtt gataactctg
      481 tacctttaga atgttatgaa atgattaata aattaactga aaaagaacaa attgcaatgc
      541 tagagttaat aaagtgcatc attaaataca aagaaatata agtgagggaa atagtaacaa
      601 taatgaatta tataggtaaa agacttaaag aagaacggaa aaaagcaaat ttaacttcaa
      661 aagaatttgc aaatatggtt ggagtatcgc cttggtatat aactcaaatc gaaagtggta
      721 aaaagaatcc aagtcttaaa acattcataa aatttgtaaa tatattaaat atttctgcag
      781 atgttcttat aaaagatatt acttccactg gaaaaacata tttagaaaat gatataaatg
      841 aagagttaaa agacttaaat tcaagagaac taaatctgat atcacagatt atagacatca
      901 taaaaaagaa taaaatataa ttctttatat tttatataaa aaaaaaggtt atttgaaata
      961 aataaccttt ttttgtcttt ttgtgttaac attgtcttat agtgctaatg agtactatta
     1021 gaccatgttc tatggcctta ttttaatata aaacgattgg agataggcta tgattgtgat
     1081 tgaaggtagc gataaattta agattgcaaa agaatatatt gatgtagaat atactctttt
     1141 tagcaaagta acttttaggt atgaaaagtt gaaatttaaa gataatgctg aattggaaaa
     1201 aattaagatg tttaaatata aaaatggcta catccctaat aaattaaacc tttcttttgg
     1261 atatggattc tcttcttata aaaagcaaat aattagagaa actgtagata ctttaagatt
     1321 gacagaaatt ttttcaagcg agaacataga agatattaaa tttataaaag atggtacaaa
     1381 aaaattagaa attagcatag agaaagttgt gaaatttaaa cgtcgaaaaa aaagaaatta
     1441 tgtttgttgc tattgccctg atatgtatag agacataaaa ctcgacaaag aatctatcaa
     1501 taagatatac aacagaaaaa taaaaataga aagagaagtt aatatttttg aagatgagga
     1561 tgttataata aacaagaaag tattgaagtt tccaaagtct tggacaaaga atatgcaaaa
     1621 atattggtta agtgaaaata agtatcccat acattctact gtaattgatg atgatagata
     1681 taaatgttgt aatgtacaat atacaaaaaa tagagtgata atattatatt acatatataa
     1741 ccattaatat acataattac aaaaaaataa tgacaaatat acataataca aatatatctt
     1801 ctactttaga taaaacagtt attatgaaaa cttactatct ataaataata gaaatgctag
     1861 tataccatct ataatctaat gtctagtagg ttttaatggc atattttaaa tatgccatag
     1921 ttgatttata agattttcta tatcactttc aaaatacaca ctaaatagtt gcttttcaga
     1981 tataattgcc caatttaaat tttactatct gaaaagtttt ttatatttaa tttatatctt
     2041 caaaaatacc gtctaatatt tctatataat taatttaatt aatactctta tgtaatagat
     2101 taagtactga atttaactct ttaaatgata tttgccctgg tgctgaaaca ctttttgcag
     2161 ctccaaaagt caatgcagaa ccaaatattt caccacacaa tctacttata acacccattc
     2221 ctgacattga cattgttatt attggtctat ctgcataaat tttaaacatt tcatttgtag
     2281 cttcaagtag cactaacaca tctttttcat tttgcggcat aacagctatt ttgggtaaat
     2341 ctgcgcccaa ttcctgcatt ctacataaac gagatacaat ctcttctttt tttggagttt
     2401 tattaaaatc atgattagat ataattactt tcacttcttt tttatgagca aaattaacta
     2461 cttcatctat gacctcatct cccataaata actctacatc tattaaatct actaaacctg
     2521 tattagaaat ttctttattc aaagtagtat aataatctct tgatattaat ttttctcccc
     2581 cttctactac acttctaaat gtgaataata aagggatatc atgtatataa cttcttaatt
     2641 catagagaac ttccttaact tctttaatat tttctacatt ctcaaaaaaa tcaactcgcc
     2701 actctataat atccaaacat gcatctttta actctttagc ctcttttata atatcttttt
     2761 tatttttccc aataattggt acacaaattt taggtctacc ttctccaata gtaatatttt
     2821 ttacttgaac ttttcttttc atattctcct ccataatcag aaattctata ggactttaaa
     2881 ttctattttc tgcacaattt aattatacat taaaaatact atacattata aaaatactaa
     2941 tttaaaaata atttttattc taattcttaa aacaatccca catattttag aaaaggtact
     3001 ctataatata caaacacttc ttttttacct aaatttattt taacatccta taattttcac
     3061 aatattcttt aaattttact catataaaca atattattct ggatgctaat agaattaatc
     3121 tataaatcat agtataatta tgcagaaata actcgaattt cactacttat gaagagattg
     3181 aatattaatg aatctaaatc atttgcatta cttaagagtt ttgactaaag ttgaacacca
     3241 tacaaaagta gctactcaat tgtttatacc ccaaccaagc ttaagtaatg ttatatagat
     3301 tctcgaaaaa gaatttggta tctttttatt tgaaaaactt atattaaatt tgattatctt
     3361 ttcataagtt ggtaaatgtc gatatatcct gtatctacaa ggagtatcgg cattttcttt
     3421 ttggaagata ctttgcataa gttggcattt aaccgtataa gaatgccatc aaaagtagta
     3481 aataagtagt aaactctagt catactacta ggattataac cgttccattt cagctctcga
     3541 agaactaaat gaaccatgtg cataatagcc aagcgtcata gtaatgtttt tatgccccat
     3601 aatatattgc aatatattag gactcattcc tttgttagcc atatttgtac agagtgtatg
     3661 cctaaaggta tgtggcgtaa tctttggtag cttgtcctca tgggtcttat tatacttctt
     3721 gactaacgct cttaacatgc tttgataatg agttgcttgt tttggtaatc catcttgttt
     3781 aagaaatagg aagttctggt aaccatcaac aatgattggt tctgctttag cgttcttacg
     3841 ccgttccacg atacgttcaa atgattgata agctctttca gtcattggaa tttctcgctt
     3901 accctgtgtg gtctttggaa cttctacata atagccaatc ttagtatcac tcaacaattg
     3961 atggtctacc ataatggctt tatttttcat atccaattct aaagtcaaac cgcataattc
     4021 agaaatgcgt agtcctgttt ctaagaggat aacaacatca tcataatatt tacaataagt
     4081 cttatcgctc cccataaagg ataataagcc catttcctgt tctggtgtta gagcgatttt
     4141 tggttctgtt gtatcttcca aaacatcact taactaaaaa ttaaatggat tctttctaat
     4201 acagtcgtct tgaattgcca tgaaaaacga cgctttcagt gagcgcttat aattgtcaat
     4261 ggatttataa gcacacccat cttcactcat tcggatagcc cagcttttag cgtctgataa
     4321 tttaaccata tcaatgcttt ttgcacctag tgggtcattg atagtatcta tattttgatt
     4381 tataatttat acattttttt aaaaacagac ttttaactgt aaagaatcta tgtataaact
     4441 tagctatgtt gtccgtgtat ctacaaagaa tcttgacgag ctaaaaaggc gttgtgatga
     4501 agtcaaagac ttttacgacg acttatcaat caagctcgtg cgtccctttg gggatatgtt
     4561 gggattgcat ggcgaattta ttcctgtcag caaacgttac atcaatgatt atattcaata
     4621 tgtcacttcg gattttttgg ctggacttgg ttttggagcg acacaaaccc ttggcgaaca
     4681 tgaggatatc tatcttggct acaatctgga cactggaaag aatgtctatc taaaacctgc
     4741 ccttgctagt caaggggtaa agggttctat taccaatgcg ttagcgtctg cgtttatcgg
     4801 ttcattgggt ggtggtaaat tattcagtaa caatatgctt gtttactatg cggtactatt
     4861 cggcggacag gcggtcatag ttgaccctaa agcagaacgc ggcaaatgga aagaaacgct
     4921 accagaaata gcccatgaga taaacattgt caatttaacc agtaatgatg aaaacaaagg
     4981 attgcttgac ccttacgtca ttttgagccg taaaaaggat tctgaaagtt tagcgattga
     5041 tatcctaaca ttccttactg gtatctccag tcgtgacagt gataagtttt tgcagaaaaa
     5101 tgacaactgg aaaataataa tggaatagtt tctatgtaaa tatattttta ctatcttacc
     5161 taactaattg aaagatgata taatttaatc aaagatttgg taaggaggga tgaatatgca
     5221 tttaattgat tttatgaatg aagatattaa aaagaagctt gttaaaaaaa cgtataaaaa
     5281 gaaagagact attctttttg ctgagcaaga aaatgaatat gtaattctta tgattgacgg
     5341 tattgcagaa gcattcatct taaatgtgaa aggaaacatc tcaacgattc atctttatag
     5401 aagtggtagt ttttttggag aaatggaaca atttaatgat gggaaaaaac ccgttgaaat
     5461 tgtcgctttc acagattgta atgtatataa acttcataga gatgattttt taaactggtt
     5521 aaggggagat tttgaagcta caaaatttct gattcgagaa atatctagta aattagttgt
     5581 aaatgctgaa ttaattgaag aattatcatc tttaagtgtt aaagaacgac ttctgaggtg
     5641 tatatcctta cactattata gaagtgaatt aaatctgctt accaaaaaac agcttgctag
     5701 cgaagtaaac gctccaatta gaagcattaa tcgtgctata aatgaatgtg tacaagaaca
     5761 tctaattgct tatgaaaaca aaagatttag tgtaattaat cagcgagagg tattaaagaa
     5821 tcttcctaca acataagtat aatttcaaaa aagtattgaa taataattat tcaatgcttt
     5881 tttatttcgg gtcaagtgac ctattttttt ttatttcttc attatataat actcctatgc
     5941 ataattatgt tggattgata gctaataaaa gacagtgaga ggtgttatat tatgactaaa
     6001 acacaacaga aaatgataca aaacgtatta gaattttcaa aaagagatag taggataata
     6061 ggacttgcaa ttagcagttc ttatatcaca aaatcgttag atgaatattc tgatttggat
     6121 tttctgattg tagcagatga taaatcctat gaagaagtta tcgaagagag attacttatt
     6181 gtggaacagt ttggaacatt agtttctgct tttactggcg aacacgttgg agaacctcga
     6241 ttgataattt ccctctatga ttcccctgtt cttcatgttg attttaagtt tacaacctta
     6301 gatggtttac tagatagggt tgaagattca gctattctct atgaagaaaa caactgcata
     6361 acagaaattt attctaaaaa agaagcatac ttcccaactc cagacttaca gtggattgaa
     6421 gatagatttt ggatttgggt tcattacgct tgcactaaga ttggacgtag agaactattt
     6481 gaaacaattg attttttatc ttttttaaga caatcagtac ttgccccatt aatacagctt
     6541 ctcttaggta aattacctcg tggagttaga aaaattgaat tggacgcacc ccaatatatt
     6601 gaagcattag aaaagacggt tgctaaccat gaatataaaa gctgtgttaa tgcattgcag
     6661 gctactatca acctgtatct tgagttaaga gaggaattat cacaaagtcc tattgtccga
     6721 aaggttgaag ctgaaagggt tgcattacag tattttcaag atttaatcaa ataaacccaa
     6781 agtaaaaaaa tattgaatag taaatattca gtgttttttc atttttggtc aaatgaccta
     6841 gattagttgt tgtaacaatt ctataattat tttgaaagta acagtatgga ggataactat
     6901 gagtgatatt ttaagtagaa ttacaaataa aattggggat aagaatatag ttgataagtt
     6961 atcagctcta tcaaaatcag atttgaactc acttctatta gaagtgtttg atagacaagc
     7021 aaatacttta actgcaactg atattctaaa gtcataccaa ctaaatagat ttactatccc
     7081 tagcggtata gatccagaag aaattcatgc tttggaatca aaattattga gaaaagcatt
     7141 caatatggat atcaagacga ttatgctttc tccctctgcc ccattaggta gctgtagcgt
     7201 ttttggctcc gtagatcaat ataacgtagt aagtgctcta cgagggacag aaacacttgc
     7261 agacccctca aatatgttgg caataattat tgctgataaa ttaaaaagaa aagaagaaaa
     7321 taataccata cctatccaca tggctactac tgctagggta ataagagcac aaaattttgt
     7381 aggtaaaggt ttatattcac attttggctt atattgtatg gtttcatctg gtatagatac
     7441 agggtcttac acttgtgaaa aaatgttgtt agaaaagcac ttaaactatt ataaagatat
     7501 attaagtgaa atagaaaatg ttcgtttctc tattattcta cgcaaaagaa acggatacaa
     7561 agataatgac ggatttttcg acagaatggt tgaaactatc aaacttattt ttcctaatat
     7621 agagttatca attcataatg atgatgttga taataattat tataaaggta tcaatttcaa
     7681 attatcggtc aaacaaggaa atgaaaccgt tgaaatagtt gacggtggat ttgttgactg
     7741 gacttatcaa atgcttggaa ataaaagaga acgctgtttg ataagtggta ttggcttaga
     7801 aagatttctt gtagctgttt cataaaagga gaagaaaaat ggaacttata gagtggaatt
     7861 atgcttatat taccgattta gttgagtttg cgaatgataa agaaattgca aagaatcttc
     7921 gtgacaactt cccccaccct tatagggagc aaaacgctag agattttgtt agtttttgtt
     7981 tggcaacttc agatgttaaa caacttagct atgctatttt ttatcaagaa agggctattg
     8041 gaagtatcag cctaacattt gaagaagata cctctattaa taaaaatgct gaattaggtt
     8101 actggcttgc taggaactat tgaggcaaag ggatagcaac aaatgcagta aaagaaatat
     8161 gcaaaattgc ctttgaaaaa tacaacctta acaaaattca tgctaatgta tattcctaca
     8221 atagagagtc aagtaaagta ttggaaaaat gtggctttaa gattgaagaa tactgtcaca
     8281 attcataata aagaatagaa acatgattga ttgccatgta tatagtttaa ttcgaccgca
     8341 atagcattta ttagaattaa cctacttttt aataacagaa taagtttttc ccctctatta
     8401 atttcttatg catctttgta tttttatgaa aagctagcca atgcatattt ccatacatca
     8461 atatggtggt ggcttacaac caatataaat taaatacgtt ttaaagaata cacatttttg
     8521 ccctataggc agagtgtgta tttttttctc ttttttttga aatttatcga agatttaacg
     8581 ccctcccacc gtgataggag taagcaaggt tatttaactc cttgcgatgg aaagagggtg
     8641 aaatcttatg gttgatgaaa agacaattga acatcaattc gacagtttct gtaaaacagt
     8701 tttgcgtaac tatgctagag atatttatga cgaaaataaa cgcagaaatg attatttggt
     8761 atctctcgaa tctttgtcac ttgcagaatt aagtaaattg agtattttag atgattatga
     8821 ttctaactac atctgtatgg tttcttacga ctacaacata cgaattgaag atgtattaat
     8881 ggcgcaagca atcggaaaac ttacaaaaag aaaacaagat ataattctac tttcattctt
     8941 tttcaacatg acaaatgctg acatagcaac attgatggat ttagcagaaa ataccataca
     9001 ttatcacaaa acaaatgcac taaaagagtt gaaagaactc atgcaggaac attgaaatga
     9061 aacctactaa agaagtatta ccttttacaa ttattttact tgctgtacat ggcgatgtaa
     9121 atgcaataaa cgtaatactt aaacattttg aacactatat cattcgtctt tcacagaaaa
     9181 ctttatttga tgaatacgga aatccctata ttcatgtaga agaagaaaca aagcgaatgc
     9241 taggaacaaa actaatcact gctattttga gatttaattt gtcttaatct cgccctctct
     9301 ccatgtatca agctatttgg tacatggaga atcactaatt atttgtctat aaaagcccac
     9361 aagctattgt gattttttat aggcaaatca atgccgactg ttctttgaca actgaataaa
     9421 gtagatagat acgtttgtga tacagatagc caacaaatac tatacgccat gaccttaatc
     9481 aagagagcta ctatatagtg atttgtgaat aacctttgga acggttgttt tgccgtaacc
     9541 tgtaccacag tataatgata cacctgtatg acacggttct gtccataaga atgggattgg
     9601 ttgaaatacc aatggagctt atcaaagcca tctatctact tctgaactac gaacatagaa
     9661 aggagtgata cattatatga acaatactga tgtacctatt tgggaaaaat ataccttgac
     9721 gattgaagaa gcgtcaaaat actttcgtat tggagagaaa aaattacgta aacgtgctga
     9781 tgaaaacctt gattctggct gggtcattat gaatagcaat cgcattcaaa taaagagaaa
     9841 acaatttgag aaaatcgttg atacacttga cgttatctaa tgaaattgag ccttatatgt
     9901 tataatattc atatgacgta tcaagctcaa ttcggttatt gagaggagct tcttatcatg
     9961 tcaactgaaa aaagacgaga taataaagat agaattttaa gaactggaga gagccagaga
    10021 aaagatggaa gatacgcata taagtatgta tattcatttg gtaaaccaca atttgtttac
    10081 tcttggaaac ttgtacctac tgataaaccc ccaaagggaa aacgtgatga tatatcatta
    10141 agggataagg aaaaagcaat acaaaatgac cttgatgata gcattgacac tattggtaag
    10201 aaaatgacag tttgtcagtt atacgctaaa aagaatggac taagaaagaa tatccgactt
    10261 aacactaaaa aaggacgcca ttatcttatg aagattctta acaaggagtg attctgtgaa
    10321 gagatgtatt gtagtagtgg aatagtcaaa aacataatat atattaaagg agagatttaa
    10381 atatgactat tccaagttca aataaggtat atccaagaag taatgattat caaactgtat
    10441 atcttaaaaa tgtaataaca agggaaaata tagaagttgg agattacaca atatataatg
    10501 acttttataa tgacccaaga gattttgaga aaaacaatgt attgtatcat taccctataa
    10561 acaatgataa attaataatt ggtaagtttt gctcaatagc atgtaatgca aaatttctaa
    10621 tgactagtgg aaatcactca atgaaatcaa aatccacata tacttttccc atcttttatg
    10681 aagaatggaa actcgatatc aaccatataa cggacgcttg ggacaataag ggtaacatta
    10741 taattggaaa tgatgtgtgg ataggttatg attctataat tatgtcaggt gtaaatattg
    10801 gtgatggtgc aattattgga acaagagcag tagttactaa tgacgttcca ccgtattcta
    10861 ttgttggagg agtacctgca aaaattataa aaaagagatt taataatgat acaatttcaa
    10921 aattattaga aattaaatgg tgggattggc catataaaaa aattcaatca aacattaagc
    10981 atattcaatc aggaaatatt gataaattag tttaatattt tataatttat ttatattaaa
    11041 c
//
LOCUS       DAYAPA010000305         8658 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h168-18
            SAMN40601873-rid21575613.guided.154, whole genome shotgun sequence.
ACCESSION   DAYAPA010000305 DAYAPA010000000
VERSION     DAYAPA010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601873
            Sequence Read Archive: SRR28438763
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157519.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157519.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:14:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,951
            CDSs (total)                      :: 3,867
            Genes (coding)                    :: 3,834
            CDSs (with protein)               :: 3,834
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 25 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 8 of 33
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8658
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601873-rid21575613.guided.154"
                     /isolate="ST8-SSI-h168-18"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2018"
                     /collected_by="Statens Serum Institute"
     gene            278..1231
                     /gene="blaCDD"
                     /locus_tag="ACGI59_003945"
     CDS             278..1231
                     /gene="blaCDD"
                     /locus_tag="ACGI59_003945"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDD family class D beta-lactamase"
                     /protein_id="HFG9741644.1"
                     /translation="MKRKKNFIWIAILLVGVVMVMYYVGKKHNDINQKTDKNYIKSEL
                     NKSELNKSKIENNNKNKKEKINMVDYSDCFEGISGGAIFYNTKNKEYNIYNKELIETR
                     RSPCSTFKIVSTLIGLEKGVINSKESVMGYDGTEYPNKNWNKNLSLEEAFKESCVWYY
                     KKLIDKVDAKSVQNILDDLKYGNCDISEWEGDLKNGKGHLNGFWLESSLQISPKEQVQ
                     TMAKIFEGDTNFKKEHINILRDIMKIDVNDKNINVYGKTGTGFDEKNKRVDAWFVGML
                     EREGDTYYFAIKSDDSNKEITGPKVKEIAINIIKKYYSVRE"
     gene            1575..2258
                     /locus_tag="ACGI59_003946"
     CDS             1575..2258
                     /locus_tag="ACGI59_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFG9741645.1"
                     /translation="MEILKCENLTKIYGSNQTRVTALNNVNLSVQKGDFVSIVGASGS
                     GKSTLLHLLGGVDRPTSGKIYVEDTEISSLKEEALAVFRRRKVGLIYQFYNLIPTLDV
                     RKNILLPMLLDKRKVDEDRFSEIVSILGLSDRLNHLPSQLSGGQQQRVSIARSLIYRP
                     AILLADEPTGNLDRKNSEEIVDLLNLSNKRFNQTILLITHDEKIALEANRIVTMEDGV
                     IVSEKVVKK"
     gene            2255..4915
                     /locus_tag="ACGI59_003947"
     CDS             2255..4915
                     /locus_tag="ACGI59_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFG9741646.1"
                     /translation="MNILQEYTLDFVRRNKRSSIAIMLAILLTTTMMSSLCGLLYTMW
                     TDFIKLSIEKEGNWHGELFDYTYGSDLPLIENFSSVDDVMVKGQWYVGKINDEKRDYI
                     IYRNATSEYWNSMPEKDTIIEGRRPNNANEIALSKQYFENNPSVKIGDKITLPIGDRI
                     LNGKPLEPIAPKSKGESFKKISEATLTIVGKVDVSTSSVIPAYTALGYLDKTSIKPND
                     SITVYLRFKNIRDTYKELPKLAKSLGWKTNEYGKYNLKYNSNYLLKMLVFSPEQKASM
                     SSISKFSTPIMYLTIAIFTVAVFVMVIYNAFSLSANARLTQLGILSSVGASPKQIKRS
                     VVFEGFLLTIIPLPIGLFLGWLLCNRLIVYVNSVNYHTDVPEVVFTYGIPAFLPAVLL
                     TIVTVWISALIPARKVSKISPIEAIRQGDSVKIKKSHKYSLGKIFGIEGELASNALRA
                     RKKSYRTATISLTLSFLLLTCFLHIIANQEGAKAVYGSDNYKDQRDIAVYLENSEPVE
                     SAFIEKLDNTEGVKSSLYYQKMSASTWLTEKDASKELAKNGGFKNIASSKKYSPIERD
                     GKFRIISNIIGLDDKSFTEYCRQISVDPKLFYDTKNPTSIVYNKEEDINRSTRRNKVY
                     IDYLNLSIGDNIKLNEKVYDEDKGDYTYNMKVGAITSTLPKIVDTSSKYTLMQIVPMS
                     MVQEISKNYDEVSRLRSNRLIGVFKVNNREDIPRVREKLESIYNKDYGSGDYSIEDVL
                     ESEKQKASGIKTMNLIVGFITGLLAMIGLSNVLATVSGNIRSRRQEFAMLRSVGLSPE
                     GIKKMLILEGLFLGITPLLLSIPVQIGIVYAFLRINEIYFIEYLPFAPISTIIGFTIL
                     ILFIVIASYMTGYKQLKNENIVESIKNETI"
     gene            complement(5096..6277)
                     /locus_tag="ACGI59_003948"
     CDS             complement(5096..6277)
                     /locus_tag="ACGI59_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFG9741647.1"
                     /translation="MKTPRNINSTVVFIIFCLIVTFLLSVILTTITSFNEYNVLSKAL
                     GASYEYNPKSTESILKSLKNSSDKDFKTGNEILSKHGYSKSTFWSKNFFYFLVISFGI
                     ISTMATVVYIIKHRTNKQKLSRINNLTQYLEEVNLGKESVLLRCEDEFSHLEDEIYKT
                     VGELRCSKEFALKERQTLSDNLADIAHQLKTPITSMSLMAQLLSENCPDEEIDYINRL
                     TNQISRLERLVSSLLTLSKLDASTLIFESKLIDVHSLLTYAIEPIEGSLREKHQTFTI
                     EASPSVEFEIDINWTLEALLNILKNCSEHIEDGGYIVAYYSQNPLYVEIIIEDNGKGF
                     AQEELPYIFNRFYRGKNASKDSIGIGLALSKSIIERQNGTLHAENCHNGGARFIIKFY
                     T"
     gene            complement(6277..6939)
                     /locus_tag="ACGI59_003949"
     CDS             complement(6277..6939)
                     /locus_tag="ACGI59_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFG9741648.1"
                     /translation="MAKILLLEDNDTITFGIKTSLEKKNHTVCCYNTIAEAKKNFDKT
                     IDLILLDLNLPDGIGYEFCQYVKSIEDTPVIFLTVRDDEKDIIRGLDMGADDYVVKPF
                     LLSVLQSRINAVLRRTHSNTNNTITCENISLNKVEVKAYLDNVEVPLTAGEYKLLLSL
                     MENKNRAMTRTKLLEMLWDVDGNFVNDNTLTVTIKRLREKLKNPPCIKTLRGIGYRME
                     DN"
     gene            complement(7071..7709)
                     /locus_tag="ACGI59_003950"
     CDS             complement(7071..7709)
                     /locus_tag="ACGI59_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFG9741649.1"
                     /translation="MSELSKRDKEKIQRENEIIDKAEKLFCLNGFDNTTMNELAKEVE
                     YTKRTIYKYFSCKEDLFFAVVLRGYKRLWDNVKIESAKGKTGFEKIKLSYFAFHKFYS
                     VEPSLLCLMGMIGIVNSEKSDTDMLFKEKFFSFNKFMFDEIQGMFEIGKNDRSIRHDI
                     EIPTLMYSSIFTLTGFFNLLSVTGKSYLNNFNIDEEKFIETTLALLIDSIKA"
     gene            7888..8493
                     /locus_tag="ACGI59_003951"
     CDS             7888..8493
                     /locus_tag="ACGI59_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFG9741650.1"
                     /translation="MKIKENVLMLDSTRGSNCFIVFDKEIILIDTGLPFMGKNIIKEL
                     KTLGIKLTDIKHILLTHHDVDHISNIKLLKEKTGAKVWAHVEDIPFIIGEKDRPGFKK
                     FIGKAISRRLIKDVEPYGENMQIGNIKIIHTPGHTPGHVCMIFEDVLFAGDLVKNKNG
                     NIVPYPNPWNWDYKKMMKSIRELDNLKYEWICMSHGTPCIK"
BASE COUNT         3217 a         1076 c         1353 g         3012 t
ORIGIN      
        1 attgtaatat ttaaatatta caatagtaag ttttaaatgt ggaatcaatt attatatcac
       61 aaattaaaag gaggacagga tgttattaat agttacttaa ttatcttcgc aataggttta
      121 atcaacataa ttgtactagg tgaagaaaat ataagagata cagctattca tttagtcttg
      181 tttcttataa gtagaaccta gattggagta tattacaaat aatgtctact attttaatag
      241 taggttataa atttaaatat gggagatgat ataaattatg aaaagaaaaa agaattttat
      301 ttggatagct atcttattgg ttggtgtagt tatggtaatg tattatgtag ggaaaaaaca
      361 taacgatata aatcaaaaaa ctgataagaa ttatataaaa tctgaattaa ataagtctga
      421 attaaataaa tccaaaattg aaaacaataa taaaaacaaa aaagaaaaaa ttaatatggt
      481 tgactatagt gattgttttg aaggtattag tggaggagct attttttata atactaaaaa
      541 taaagaatat aatatatata acaaagaatt aattgaaaca agaaggtcac catgttctac
      601 ttttaaaata gtttcaactt taattggttt agaaaaaggg gtaataaact caaaagaatc
      661 agttatggga tatgatggta cagagtatcc aaataaaaat tggaataaaa atcttagttt
      721 agaagaggca tttaaggagt cttgtgtttg gtactacaaa aaattgatag ataaagttga
      781 tgctaaatct gtccaaaaca ttttagatga tttaaagtat gggaattgcg atattagcga
      841 atgggaagga gatttaaaaa atggaaaggg acatttaaat ggattttggt tagaatcttc
      901 attacaaata tctccaaaag aacaagtgca aacaatggca aaaatatttg agggagatac
      961 aaactttaaa aaagagcata ttaatatttt aagagatatt atgaaaatag atgtaaatga
     1021 taaaaacata aatgtatatg gaaaaactgg aacaggattt gatgaaaaaa ataaacgtgt
     1081 ggatgcatgg tttgttggta tgctagagcg tgaaggtgac acctattatt ttgctattaa
     1141 atcagatgat tccaataaag aaattacagg gccaaaggta aaggaaattg caattaatat
     1201 aattaagaaa tattatagtg taagggaata aacttaattt tatttgtaaa tattatttac
     1261 ctatagtctg taatatttat taaataattt tactaaattg agtctatata gagcaaaact
     1321 cctttagtac ataaaatact tgctattgat tattaaattg aatacctata gatttaaagt
     1381 taataccttt taaagactat gtaatatatt aaaagtataa ataaaatata ttgcatagtt
     1441 tttgttttat attataattt taaataatgt gcaaaaacta aacaatattt ttaatagtaa
     1501 tttatatttg tattcatgtc actataatgt cattttaggt tgctaaaatt taaccataaa
     1561 ggagatgatt gtatatggaa atattaaaat gtgaaaatct cacaaaaata tatggttcaa
     1621 atcaaaccag agtgacagcc cttaataatg taaatttatc agttcaaaaa ggtgattttg
     1681 tatccattgt aggagcatct ggttctggca aatctacatt gttacatctg cttgggggag
     1741 tagatagacc tacatctgga aagatttatg ttgaagatac agaaatatca agtttaaagg
     1801 aagaagctct tgcagttttt agacgtagaa aagtaggtct tatttatcaa ttttataatc
     1861 ttatacctac ccttgatgtt agaaaaaata tattattacc catgttactt gataaaagaa
     1921 aggtggatga ggatagattc tctgagattg tatctatatt gggattatct gatagattaa
     1981 accatcttcc aagtcaactt tcaggtggac aacaacaaag ggtatctata gctagaagtt
     2041 taatctatag accagctatt ttattagcag atgagccaac tggtaatctt gatagaaaaa
     2101 attcagaaga aattgtagac ttgttaaact tatcaaataa acgatttaat cagacaatac
     2161 ttcttattac tcatgatgag aaaatagcac tagaggcaaa tcgtattgta actatggaag
     2221 atggagtgat tgtctctgaa aaggtggtga agaaatgaat attcttcaag aatacacctt
     2281 agactttgta cgacgcaata aacgtagtag tatagctata atgttagcta tattacttac
     2341 tacaaccatg atgtcttctt tgtgtggact gttgtatacc atgtggacag attttataaa
     2401 actttcaatt gagaaagaag gtaactggca tggtgaattg tttgactata cttatggaag
     2461 tgacttacca ttaattgaaa atttttcttc agttgatgat gtaatggtaa aaggacaatg
     2521 gtatgtaggc aaaattaatg atgagaaaag agattatata atttatagaa atgctacatc
     2581 agagtattgg aattctatgc cagaaaagga tactataata gagggtcgtc gaccaaataa
     2641 tgctaatgaa attgcattat caaaacaata ttttgagaat aatccaagtg taaaaattgg
     2701 tgataaaatt actctcccaa taggagatag aatcctaaat ggaaagcctc ttgaacccat
     2761 agccccaaaa tcgaaagggg aaagttttaa aaaaatttca gaggctacat tgacaatagt
     2821 aggaaaggta gatgtttcta cttcatcagt aatacctgca tatacagctc ttggatatct
     2881 tgataaaaca agtataaaac ctaatgatag tataactgtg tacttgcgat ttaaaaatat
     2941 aagagacacg tataaggaac ttccaaaact tgctaaatca ttgggatgga agactaatga
     3001 atatggaaaa tacaatctta aatacaatag taattatctt ttaaagatgt tagtcttttc
     3061 accagaacag aaagcatcta tgagtagtat atcaaaattt tcaactccta ttatgtatct
     3121 tacaatagca atttttacag tagccgtgtt tgtaatggtg atttacaacg cgttttcact
     3181 gtctgcaaat gctagactta ctcaattagg gatattatct agtgttggtg catcaccaaa
     3241 acaaattaaa aggtctgtag tgtttgaagg ctttttactg acaattatac cattaccaat
     3301 aggtttattt ttaggatggt tattgtgtaa taggcttata gtttatgtta attcagttaa
     3361 ttatcatact gatgttccag aagtggtatt tacttatggt attcctgctt ttctgccagc
     3421 agttttactt acaatagtaa cagtctggat ttctgcactt ataccagcac gaaaagtatc
     3481 aaagatatct cctatagaag caatacgaca aggagatagt gttaaaatca aaaagtcgca
     3541 taaatattca ttaggaaaga tatttggaat agaaggagaa cttgcatcaa acgcactacg
     3601 tgctcgtaaa aaatcttatc gtactgcaac aatatcttta acattatctt ttttattact
     3661 tacatgtttt ttgcatataa ttgcaaatca agaaggagca aaagctgttt atggaagtga
     3721 taattataag gaccaaagag atatagcagt ttatttggaa aatagtgaac ctgtagagtc
     3781 tgctttcatt gaaaaattag ataatacaga aggtgtaaaa agttcactat attatcaaaa
     3841 aatgtctgct tccacttggt tgactgaaaa agatgcttca aaagaacttg cgaaaaatgg
     3901 aggatttaaa aatatagctt catctaaaaa gtactctcct atagaacgtg atggcaagtt
     3961 tcgtataatt tcaaatataa ttgggcttga tgataagagt tttacagaat attgtaggca
     4021 aatttctgta gacccaaagt tattttatga tacaaaaaat ccaacctcaa ttgtatataa
     4081 taaggaggaa gatattaatc gtagtacacg tagaaataaa gtatatatag attatttaaa
     4141 tctttcaata ggagataaca ttaaattaaa tgaaaaagta tatgacgaag ataaagggga
     4201 ttatacttac aacatgaaag tgggagctat tacaagcaca cttcctaaaa tagtagatac
     4261 tagttcaaag tatacattaa tgcaaatagt tccaatgtct atggtgcagg aaatatctaa
     4321 aaattatgat gaagtaagta ggttacgttc aaataggctg ataggagtat ttaaagtaaa
     4381 taatcgtgag gacattccta gagtaagaga aaaattagag tccatttata ataaagacta
     4441 tggttcagga gattatagta ttgaggatgt tttagaaagt gaaaaacaaa aggctagtgg
     4501 aataaagacc atgaatttaa tagtaggctt tattacaggt ttattggcta tgattggatt
     4561 atcaaatgta cttgctacag tgtcaggaaa tattagaagt agacgacaag agtttgctat
     4621 gttgcgttct gttggattat ctccagaagg cataaaaaag atgcttattt tggaaggttt
     4681 attcttagga attacaccat tactacttag tattcctgtg caaataggaa tagtgtatgc
     4741 ttttctacgt ataaatgaaa tatattttat agagtattta ccatttgcac ctatatctac
     4801 aataataggt tttactatat tgatactttt tattgtaata gcatcatata tgacaggata
     4861 caagcagttg aagaatgaaa atatagtaga atctataaaa aatgaaacaa tataaacaaa
     4921 atctatttga acttatttaa aggtttcgat ttaagactac ttacaagtat agggatagtt
     4981 aagttgtttg tttaataaaa ccttaaatgt aaatagaagt atattactaa aacaatagat
     5041 aacatagaat atagatatag ctggattata ggtattttac ctataatcca gcattttatg
     5101 tgtaaaattt aattatgaat cttgcaccac cattatggca attttctgca tgtagagtac
     5161 cattttgacg ttctataata gactttgata gagcaagccc tataccaata ctgtccttac
     5221 tagcattttt accacgataa aatctattaa aaatatatgg aagttcttcc tgagcaaacc
     5281 ccttaccatt atcttctatg ataatctcaa catatagagg attttgacta taataagcaa
     5341 ctatatatcc accatcttct atatgctcac tacaattttt aagaatattt aaaagagctt
     5401 ctaaagtcca attaatatcg atttcaaatt ctaccgaagg agaagcctcg atagtaaatg
     5461 tctgatgttt ttctcttaaa gaaccttcta taggctcaat tgcataagta agaagagaat
     5521 gtacatctat aagtttgctt tcaaatatta aagtacttgc atccaatttt gataatgtaa
     5581 gtaaagatga aactagtctc tcaagtcttg agatttgatt tgtaagtctg ttaatataat
     5641 ctatttcttc atctggacaa ttttctgaaa gtaattgtgc cataagtgac atagaggtaa
     5701 ttggagtctt aagttgatga gcaatatctg caaggttatc tgaaagagtt tgtctttctt
     5761 ttaaagcaaa ttcttttgaa caacgaagtt ccccaactgt tttatatatc tcatcttcaa
     5821 ggtgagaaaa ttcatcttca catcgtaaaa gtacagactc ttttcctaaa ttcacttctt
     5881 ctaagtattg agtaagattg tttattctac tcagtttttg cttatttgtt ctatgtttga
     5941 ttatatatac aactgtagcc attgttgaaa ttataccaaa cgatatcact agaaaataaa
     6001 aaaagttttt actccaaaat gtacttttag aatatccatg ttttgataaa atttcatttc
     6061 ctgttttaaa atccttatca ctagaatttt tcagtgattt tagtatactt tctgtagact
     6121 taggattgta ctcatatgat gcaccaagag ccttagacag tacattgtat tcattaaaag
     6181 aagttatagt tgttagaatt acactcaata aaaaagttac aattaagcag aatattataa
     6241 aaacaactgt actgttgata tttcttggag ttttcactag ttgtcctcca ttctatatcc
     6301 tatgcctctt aatgttttta tacaaggtgg attttttaat ttttctctaa gacgttttat
     6361 tgtaacagta agagtgttat catttacgaa attaccatct acatcccaga gcatttctag
     6421 taactttgta cgagtcatag ctctattttt attttccata agacttagaa gaagtttata
     6481 ttctcctgct gtcaaaggta cttcaacatt atctaaatat gctttgactt caaccttatt
     6541 caagcttata ttttcacatg taatagtatt gttagtattt gaatgagttc ttctaagaac
     6601 tgcattaatt ctggattgaa gtactgagag caaaaacggt ttaaccacat agtcatctgc
     6661 acccatatct aatccacgta taatgtcttt ttcatcatca cggactgtca aaaatatcac
     6721 tggagtatct tctatacttt taacatattg gcaaaactcg tatccaatac catcaggtag
     6781 atttaaatca agcagaatta aatcaatagt tttatcaaaa ttttttttag cttcagcaat
     6841 cgtattataa caacatacag tgtgattttt cttttcaagg gaagttttta ttccaaatgt
     6901 aattgtatca ttatcttcta aaagaagtat tttagccatt tacttttaat cctttcagta
     6961 atttagttat tccattaact aagttattag ttttatgttt aatatttttc atattagaga
     7021 tagaaaaaat tatcatttaa gtatataaac tttaatttat aaacttaggt ttatgcttta
     7081 attgaatcta ttaaaagagc aagagttgtt tcaataaatt tttcctcatc tatattaaag
     7141 ttgttcaggt atgattttcc tgttacagat agtaaattaa aaaatcctgt aagtgtaaat
     7201 attgatgaat acataagagt aggtatttca atatcatgcc taatacttct atcatttttt
     7261 ccaatttcaa acattccttg tatttcatca aacataaatt tattaaaaga aaaaaacttt
     7321 tctttaaaca gcatatcagt atccgatttt tcagagttta caataccaat cattcccata
     7381 aggcataata gagaaggttc tacagagtaa aatttatgaa aagcaaagta tgaaagtttt
     7441 attttttcaa accctgtttt gccttttgca ctttctattt taacattatc ccaaagtctt
     7501 ttatatcctc ttaagacaac agcaaagaat aaatcttctt tacatgaaaa gtacttgtat
     7561 atggtgcgtt tagtatattc aacttctttt gctaattcat tcatagttgt attatcaaat
     7621 ccattaagac aaaataattt ttctgcctta tcaataatct cattttctct ttgtattttt
     7681 tctttatctc tttttgataa ttctgacata taaattattc ctttctattg ataaaaaagt
     7741 atactgatgg tatatttatg taaatataat atttgtatac ctatagtata ctttaataaa
     7801 aaataaaatt aatatcaagt attataacta tttataactt tattaattaa ttatataggt
     7861 aaaataccaa ataaggggaa tttatttatg aagataaaag aaaatgtatt aatgttagat
     7921 agtactcgtg gctcaaattg ttttattgtg tttgataaag aaattattct tatagacaca
     7981 ggtcttcctt ttatggggaa aaatataatt aaagaattaa aaacacttgg cattaaactt
     8041 acagatatta agcatatttt attaactcat catgatgttg accatatatc taatattaaa
     8101 ttattgaaag aaaaaacagg agcaaaagta tgggctcatg tagaggatat accttttatt
     8161 ataggagaga aggatagacc tgggtttaag aagtttattg gaaaggcaat ttccagaagg
     8221 ctcattaagg atgttgaacc ttatggagag aatatgcaga ttgggaatat aaaaattatc
     8281 catactcctg gtcatactcc agggcatgtc tgcatgattt ttgaagatgt attatttgca
     8341 ggtgatttag tcaaaaataa gaatggaaat atagttcctt atcctaatcc atggaattgg
     8401 gattataaaa agatgatgaa atctataaga gaattggata atttaaaata tgaatggata
     8461 tgtatgtctc atggaactcc ttgtattaaa taataaaaat taacatggat taatagaggc
     8521 gtagttgctt gtatgtatga taataattta cagcttcttt ttaatatatc atgtatattt
     8581 gtagctgtaa gcatatttat aacaatactt acaattttgt attttaacat ctataataat
     8641 aattttagct ttgttaac
//
